LOCUS       DATMGX010000001         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_379750, whole genome shotgun sequence.
ACCESSION   DATMGX010000001 DATMGX010000000
VERSION     DATMGX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379750"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1034..1216)
                     /locus_tag="VNR65_00005"
     CDS             complement(1034..1216)
                     /locus_tag="VNR65_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17084.1"
                     /translation="MADEKEPTNLTVEECRELAVGMFEDSAALPPGPKKDELLKLARG
                     YRNLAQLKEWLSGKLN"
     gene            1690..>2370
                     /locus_tag="VNR65_00010"
     CDS             1690..>2370
                     /locus_tag="VNR65_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ17085.1"
                     /translation="MQHCGRKVSPGDIIFNDFDVAHQQFGSDVRFGSMSLMRHDLDSA
                     CKALTGREFSEARRKHLVRPGSDLMTRLLRLHKTAGQIAKSSPEILASPESMRAIEQA
                     LVHALINCLTEGASSEPTALGRRRDSIVAKFEEFLEAKPDQPIYLAEICAAIGVAERT
                     LRSCCEEHLGMGPIRYLTLRRMHLVRHALLRANHGSATVTRVATDHGFWELGRFAVVY
                     RAMFGESPL"
BASE COUNT          535 a          619 c          638 g          578 t
ORIGIN      
        1 ctactcgttc acctcgtcaa caagggcgag aagcgcaggc ggctttcgcg atcaattacg
       61 acggaaaccc tgacccaaga cggtcgacca gctgcaccag cattacaagt gttacgtctg
      121 caatcttggt atacgccccg acactcgaag gatggtattt gcttcaagga ccggcagtcg
      181 cgatatcgtg cacgacagct atttatgcat tgctgtttag cggtgtgagc cgccgcgaag
      241 gtgggagagc tgagatcggc gtcgcgcgca ttgagtgttc cagtactcaa tgacagaaga
      301 agtgcgaaga gtacggagac gacagcaaac gactgcttgt cacatttcat ggccagccac
      361 cttttaggtc gaaaaacacc gcgtcgttga agctattgca tggtcgcgtt gatggtaatt
      421 atcgaggtgc cggaactata cggaccctta cctgtcgccc gaacggagaa agtatcgcta
      481 cctctatatc cggctctcga tgtgtatacg taagaagacc tgtcaacgcg ttgtaaactc
      541 ccgtgcgccg gcctttgcga gacgcttgaa ctcaatattt cgcctctaac gctgagagaa
      601 aataaacaac tccccccgga attgatctgg atataaccga tcgagttaac gccccaatcc
      661 gacggtgatc ctgacatcgg gcactgagga gcgttttgcg ccaacgcggg cgccgctgcc
      721 aacatcataa cggcgaaagg cgccaaaaga tacagccgaa ttcttcgtga gatattcatg
      781 cttccatcct cgcgttggtg ctgctccagc gagtcaccag atcatacaga tggatcagga
      841 tcaacttgaa cgcgtatgac agcgcatgac aattaagtca gcattcgtga ctttttgcac
      901 gcttgataca tttcagccgc cagaacttgg aaagtttatg gacgctgcag ttcgccgcgt
      961 ggggagtgcc gcgggcggca cctgcaagag gcgccggtgg gttgtggaga ccgagcgcca
     1021 cttggagtcc atcttaattc agtttgcctg ataaccattc cttcagctgg gctaggttcc
     1081 tatagccacg cgccaacttc aataattcat ctttcttagg tccgggcggt agtgcggctg
     1141 aatcttcaaa catgccgact gctagttcac ggcattcttc cacggtcaaa ttagtcggtt
     1201 ctttttcgtc tgccattgat cgtgcccagc gtcacacgct tgtcgcaata tgcactcaat
     1261 gccgctatga cttataaaat tgtttttagt tgttctgccg acttcgcatg gtttggcggt
     1321 ttttgttggc ttaggatcgt ggtattttga acctttcata tggtgccgcg acctacaaat
     1381 aggcttgcgg agctttaaga ccgcaaagga tcgctaacgt cgcctgtcgg ggccaggggt
     1441 agagcaaaag tgccttggag tagagttctc gcctttagcg atccatttcc ttgtcaggta
     1501 gccattcagg ccgccgatgt agaggttttt cccaccgcta agggggggtt ttgcgccgag
     1561 gtaacccaaa tcggcatgaa ccgactttgg atgcagcgct ttcacgtaaa tttgccccac
     1621 gtaaatgcgg taagaatgat gtccagtcgg gccgtagtct gctttctgac ggacgaacat
     1681 cagcgatcga tgcagcactg cggccgcaaa gtttcgccgg gtgatatcat cttcaatgat
     1741 ttcgacgtgg cgcaccaaca atttggctct gacgttcgtt tcggttctat gtctctgatg
     1801 cgtcacgatc tcgattcagc gtgcaaagcc ttaaccggcc gagaattttc ggaggcgcgc
     1861 cgaaagcacc tcgttcggcc cggctccgac ctcatgacgc ggctgctacg tcttcacaag
     1921 acggcaggac aaatcgccaa gagcagccct gaaattcttg cttcgcctga atcaatgcga
     1981 gcaatagagc aggcattggt tcatgcatta attaattgcc tgaccgaggg cgcgtcctcg
     2041 gaaccgaccg cgcttggtcg tcgtcgggac tcgattgttg cgaagtttga ggagttcctg
     2101 gaggcgaagc cagatcagcc gatatatctc gcagaaattt gcgcggcgat aggtgtggcg
     2161 gaacgaacgc tacgcagttg ctgcgaggaa catcttggaa tgggaccgat ccgatacctc
     2221 acactgcgaa gaatgcacct tgttcgacat gctttgctgc gagccaacca tggttctgcc
     2281 accgttacgc gagtggccac tgaccacggg ttttgggaat taggtcgctt cgcagtcgtc
     2341 tatcgagcga tgtttggtga atcgccgttg
//
LOCUS       DATMGX010000002         3160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_378945, whole genome shotgun sequence.
ACCESSION   DATMGX010000002 DATMGX010000000
VERSION     DATMGX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378945"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1086
                     /locus_tag="VNR65_00015"
     CDS             169..1086
                     /locus_tag="VNR65_00015"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HWJ17086.1"
                     /translation="MAAQVVNGPSNVISSTNSAATEQVPAVEARAAAEPPKSCVSCCA
                     PLVGRYCAQCGEKVLSHSDLDWRHYVLHELPDALVHANGKLPRTFASLFARPGELAQA
                     FVSGRRTVFIGPLKLYFAVFILYAMTANFAGMGDLSLPERARLTDPTHLLDRLLEARG
                     TIAWTDPAVQERIASRAHWLSEAGTFLIYVFVALLQQVILFRAHRRYLEHLALALNVL
                     TFYLLVTAAAQLLFPWIARGHSALAEGVLQTALGFTVLPVYWYLSIRRFYGIRVPWAA
                     TAAVTITAGNALIAVTLNTAIYALLIVTS"
     gene            complement(1239..1427)
                     /locus_tag="VNR65_00020"
     CDS             complement(1239..1427)
                     /locus_tag="VNR65_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ17087.1"
                     /translation="MKNTIRQLREARNWSQGQLAEALDVSRQTVNALENGRYDPSLPL
                     AFSIAGLFKLRIEDIFKP"
     gene            complement(1439..1822)
                     /locus_tag="VNR65_00025"
     CDS             complement(1439..1822)
                     /locus_tag="VNR65_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17088.1"
                     /translation="MNPKMRQYTKELGGALAIYAALLVASIYLTRTLHPGGLAQYAIV
                     LTPMLGIVGCVWAIIRQLRRLDEMQRRIQLEALAISFAGSAFGSLAWGFAESAGAPHL
                     PTFLIWPIMAALWVVGGFISHRRYR"
     gene            complement(2128..3156)
                     /locus_tag="VNR65_00030"
     CDS             complement(2128..3156)
                     /locus_tag="VNR65_00030"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HWJ17089.1"
                     /translation="MCGRYLPGQIALGCRVSGSSGYKVNLPTFDDVETAARRIERLAL
                     RTPVLTSRTVDRQTGANVFFKCENFQRAGAFKFRGAYNALSQLPSTASQRGVATFSSG
                     NHAQAVALAANMLGIPATIVMPSDAPRSKCDATREYGGRIVFYDRHREDREKLVAHLV
                     AEGGLTFVHPYDNPQIIAGQGTAAAELFDEVGRLDALFVSLGGGGLLSGSLLAASALA
                     PDCQVYGVEPEAGNDGQQSLRLGSIVRIPVPNTIADGAQTTHIGLSNFEIIRRDVADI
                     LTATDAALVETLKFFATRMKLVVEPTGCLGLAAVWALGRQLVGQRVGIIVTGGNIDPG
                     RLAEFLRE"
BASE COUNT          671 a          963 c          897 g          629 t
ORIGIN      
        1 acggccccaa gccggcattc agcgatcaac ggctccctcc tcaaagcggc cattcggcgc
       61 ggaccgggaa ttgctatagg attggaacct attccgatct ggccgctgaa ttgcccgagg
      121 cacaacacac accggcctct atgacgcagc gtcggggcgg atggacatat ggcggcccaa
      181 gttgtgaacg gacccagcaa cgtgatctcc tcgaccaaca gcgccgcgac cgaacaggta
      241 cctgccgtgg aggcgagggc cgcggccgaa ccgcctaagt cgtgtgtaag ctgttgcgcc
      301 cccttggttg gccgttactg tgcgcagtgc ggcgagaaag tgctgtcgca ttctgacctg
      361 gactggcgcc actatgttct gcatgaactg cccgacgcgc tggtacacgc caacggtaag
      421 ctgccacgga cattcgccag cttgttcgcg cgaccgggcg aactcgcgca ggcgttcgtc
      481 tcgggacgac ggaccgtgtt catcggaccg ctgaagctgt atttcgcggt ctttatcctg
      541 tatgcaatga cggccaattt cgcaggaatg ggcgacttga gcctgccgga acgagcccgt
      601 ctcacggatc cgacacacct tctcgaccga ctgctcgagg cccgcggaac gatcgcctgg
      661 accgacccgg cggttcagga acggatcgcc tcaagagcgc actggttgag tgaagcggga
      721 acgtttctca tctatgtttt cgtcgcactt ctacagcaag tcatactgtt ccgtgcgcac
      781 cggcgctatc ttgaacatct ggcacttgca ctaaacgtct tgaccttcta cctgttggtg
      841 acggcagccg cccagctgct gtttccgtgg attgcccgcg ggcacagcgc attggcggag
      901 ggtgtgctac aaaccgcact tggcttcacc gtgcttcctg tctactggta cctgagcatc
      961 cgtcgttttt acggcattcg tgtcccgtgg gcggctacag cggccgtcac catcacggct
     1021 ggtaacgcac tgatcgcggt cacgctcaat accgcgatct acgcgcttct aatcgtaacg
     1081 agttgagccg ccgcggtgcg cgacgaatgc gacggaattc ctgtacacaa cggagttgcc
     1141 ggaatgcggt cgctcatgac cgcctggatt gggtcaatca ggccaattgc ccccctttca
     1201 aggggatgtg gtactcatgt tgattgttag tgttgtcttc aaggtttgaa gatatcttca
     1261 atgcgaagct tgaacagacc cgcgatggag aaagccaacg gcaagctcgg gtcgtatcga
     1321 ccgttttcca gcgcattgac cgtctggcgt gagacgtcca aggcctcggc caactgaccc
     1381 tgcgaccagt tgcgggcttc gcgcagttgt cgaatagtgt tcttcatcgc cgtgagccct
     1441 agcgataccg tcggtgtgag atgaatccac cgaccaccca cagcgccgcc atgattggcc
     1501 agatcagaaa cgtcggcagg tgaggcgcac ccgcggattc tgcgaatccc catgccagtg
     1561 agccaaacgc ggagcccgca aacgagatcg caagcgcttc gagctgtatg cgccgctgca
     1621 tctcatccag acggcgcaat tggcgaatga tcgcccatac gcagcccacg atgcccagca
     1681 taggcgtgag cacaattgcg tactgagcaa gcccgcctgg gtgcagcgtc cgggttaggt
     1741 agatgctggc aaccagtaga gccgcgtaga ttgcgagcgc gccgcccaat tccttcgtgt
     1801 attggcgcat tttggggttc atttccgttc tccagttgcc agataacatg gaaaggaagc
     1861 tttccacgtt gaaacaagac tgtcaagtag gctttacaag cctcgagatc caacgccgga
     1921 gaatcagaaa cgcgccgcct ccgtcacaag tgagaggcac ggaagcggtc actggttagc
     1981 ggcagtttcg ggtcacgatc gccccttgcc tgtcaccggc gaaagtgaga ttgtaaaaca
     2041 cgccggaacg gcggctttcg aggggatcga ccggcagcag gcggccaaat cgaaaccact
     2101 gtcgatatgc aattccgaaa gcccacctta ctcgcgaaga aactctgcta agcgtcccgg
     2161 atctatgttg ccaccagtca cgatgatgcc cactcgttga ccaacgagtt gccgccctag
     2221 cgcccatacc gccgccagac cgaggcaccc tgtgggttca acaacgagct tcatccgcgt
     2281 cgcaaagaac ttcagggtct ccaccagcgc cgcatccgtc gctgtcagga tatcggcaac
     2341 atctcggcga atgatctcaa agttgctcag gccaatatga gttgtttgtg ccccatcggc
     2401 aatggtgttg ggcacaggaa tgcgaacaat cgaaccaagg cggagagatt gctggccatc
     2461 attccccgcc tccggctcca ccccgtatac ctgacagtct ggcgcgagcg cgctcgccgc
     2521 aaggagagag ccgctgagca ggccgccacc accgaggctc acgaacaaag catcaaggcg
     2581 acctacttcg tcaaataact cggccgcggc ggtcccttgg ccggcaatga tttgcgggtt
     2641 gtcgtaggga tgaacgaaag tcaggccccc ctccgctact aagtgtgcga ccaacttctc
     2701 gcgatcttcg cgatgccgat cgtaaaacac aatccgacct ccgtattcgc gcgtcgcatc
     2761 acacttcgat cgaggcgcat cggagggcat cacaatggta gcgggtatcc ccagcatgtt
     2821 ggccgccagc gcgacagctt gcgcgtgatt gcctgaggaa aacgtcgcca caccgcgctg
     2881 ggatgccgtt gatggtagct gcgaaagggc gttgtacgca ccgcggaatt tgaatgcgcc
     2941 cgcccgctga aagttctcac acttgaagaa cacatttgcg cccgtttgcc ggtcaaccgt
     3001 gcgggaggtc aacactggtg tgcgaagtgc aaggcgctct atgcgacgcg cagccgtttc
     3061 gacatcatca aacgtaggca gattcacttt gtatcccgaa ctcccgctaa cgcggcaacc
     3121 cagagcgatt tgtcctggca aatagcgtcc acacataagc
//
LOCUS       DATMGX010000003         3543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_378335, whole genome shotgun sequence.
ACCESSION   DATMGX010000003 DATMGX010000000
VERSION     DATMGX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378335"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..592)
                     /gene="msrA"
                     /locus_tag="VNR65_00035"
     CDS             complement(80..592)
                     /gene="msrA"
                     /locus_tag="VNR65_00035"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWJ17090.1"
                     /translation="MFGLGCFWGAERKFWERGDGIFTTAVGYAGGHTPNPTYEEVCSG
                     RTGHTEAVLVVFDSGKISYDQLLKTFWESHNPTQGMRQGNDVGTQYRSAIYTFGDTQR
                     KAADASKAAYQKALAAKGLGAITTEIAPSGEFYFAEDYHQQYLAKNPAGYCGLGGTGV
                     SCPIGAGVSA"
     gene            complement(878..1195)
                     /locus_tag="VNR65_00040"
     CDS             complement(878..1195)
                     /locus_tag="VNR65_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17091.1"
                     /translation="MIRGFFRLVGLLLLAGGFVFMVYDGARWVADQTLRFTRFGQFWN
                     DIHQSSQQAFRAWVEGHAPWLWDSVIKVILDQPVFAVMGVLGILLMLVFRPRKPLIGY
                     SRN"
     gene            1555..1821
                     /gene="rpsT"
                     /locus_tag="VNR65_00045"
     CDS             1555..1821
                     /gene="rpsT"
                     /locus_tag="VNR65_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWJ17092.1"
                     /translation="MANTSSAKKATRKIARRTIINKSRRTQMRGAVRTVEEAIKSGDR
                     DAALKAMARAEPELMQAAQRNIIHRNNASRKVSRLTHQIAKLAK"
     gene            3039..>3543
                     /locus_tag="VNR65_00050"
     CDS             3039..>3543
                     /locus_tag="VNR65_00050"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HWJ17093.1"
                     /translation="MTNTEQDRWSRVKGRLRSSVGEDVYTSWFARMDLESVEQESVRL
                     SVPTRFLKSWIQAHYAERVLTCWQAEMPEVHRVDLTVRTAMRCAAPAKEASPAPLDAR
                     RTERSDGRAATELRAIATAPVSASHDALGGSPLDPRLTFGSFVIGRSNTLAHAAARQV
                     AEGRRGDP"
BASE COUNT          737 a         1059 c         1072 g          675 t
ORIGIN      
        1 gttaataaac cgttttttcg cgcggggagc gcgcgatgcg gaaggctgaa agaccagctt
       61 tccgcatcac aagaattcgt caggcgctga cgccagcacc gatcggacac gacaccccgg
      121 tgccgccaag cccgcaatag ccggccgggt tcttggccag atattgctga tggtaatcct
      181 cggcgaaata gaactcgccc gacggcgcga tctccgtggt gatggcgccg agaccctttg
      241 ccgccaaggc tttctgatag gcggctttcg acgcgtccgc ggctttgcgc tgcgtatcgc
      301 cgaaggtgta gatcgcggaa cgatactggg tgccgacgtc attgccctga cgcatgccct
      361 gggtcgggtt gtgactttcc cagaacgtct tgagcaactg gtcgtaggag atcttcccgg
      421 agtcgaacac caccagcacc gcttcggtat ggccggtgcg gcccgagcac acctcctcat
      481 aggtggggtt cggggtgtga ccgcccgcat agcctaccgc tgtggtgaag atgccgtcgc
      541 cgcgctccca gaatttgcgc tccgcgcccc agaaacagcc gagcccaaac accgcctgct
      601 ccaggccttg cggataaggc ggcttgagtt gatggccgct gacgaaatga ctcttggccg
      661 tggggattgg ttgcgcacgg cccggcagcg gcagcgttcg gcagtgcagt ggttttgcgc
      721 atgaacaacg tggggggcct ccggacggga tccgttcgaa ttcacgacag cgaagtggtc
      781 tctaataaag agtgtaactc taatatatgg ctctacgcag accagggcag ccccgttacg
      841 ccggacgcct cgccacagag cagcgccgcc aaccccgtca gtttcgcgaa tacccgatca
      901 acggcttgcg gggccggaac accagcatca acagaatgcc caatacgccc atgactgcaa
      961 aaaccggctg gtccaagatc accttgatga cgctgtccca gagccagggc gcgtgaccct
     1021 ccacccaggc ccggaacgcc tgctggctgg attggtgaat atcgttccag aactgaccaa
     1081 accgggtgaa gcgcaaggtc tggtccgcga cccaacgggc cccgtcatag accatgaaga
     1141 caaatccgcc cgccaacagt agtagtccca ccagtcggaa aaaaccgcgg atcatgcgtc
     1201 accttacatc tctggcgcct gaaggcgccg tcaggcctta ccggggcagc tcgggaaatt
     1261 caacctcatc agcaccttat ccgccttgcc ggccgggccc ccgagttcct ggggagcccg
     1321 gaaggcgttg acgctgcaag gcgtggcctc tataagggcg ccaatggcgg cgggcgcaat
     1381 cccgccgccg cagttctttg agcggcgctg tcatgtaagc gtatttgctt gcagagatat
     1441 ggtgttgtca gggacatctg aaaacacgaa atcgtcgatc acgcagttgg acggccggcg
     1501 cggttgaagc ccgaccactt agcgatcggt caccgccgaa ggatatttga gaacatggcc
     1561 aatacgagtt ccgccaagaa ggcgacgcgc aagattgccc gccgcaccat catcaacaag
     1621 tcacgccgta cccagatgcg cggcgcggtt cgcaccgtcg aagaagcgat caagagcggc
     1681 gatcgcgatg ccgcgctgaa ggcgatggca cgcgccgagc cggaactgat gcaggcggcg
     1741 cagcgcaaca tcattcaccg caacaatgcg agccggaagg tttcgcggct cacccatcag
     1801 atcgccaagc tggcgaaatg aactgaacga aaatgtaatt cgtcaaaagc ccggccttgc
     1861 cgggctttct atttttgggg gcaccgcact cggtaagttg cgactcgcaa aaagtccctg
     1921 cgcgtcccgt gtttttactg gcgctatgta ggaaggcggg tttagcctcc ttgccaccgg
     1981 gcttcacgaa atcgacgccg gtcatgctcg gccttgcttt tgaaattgca actgctacgg
     2041 cgcgcccgct tccgaaagca gcgcggggtt accctcttcg gacgaacaaa aaaaaacgcg
     2101 cttgcgtagt cacttatcgg catagtggcg aagagttgtt cgaaaaatcc tccccgccac
     2161 atttaaacgt aaacgattta tgaatttatt ttgggcattg tcagaaatgt cacagtcgtt
     2221 cgacgctttc gaatcttcgc gcagattcaa aaccttgcca cttccggcga aatgcgctgc
     2281 ctgatttcgc aaacgcgaag tcgttttgcg ggcgcgggtg cggaatcatt tccgttgcgg
     2341 gaaatggggc atgatgtatc tctaagggcg ttcgcggcgc ccacggctct tcagaccagc
     2401 gcggtttaag aaacggggcg ggcgtgcaat cggcggcggt gtgcgcgttg gcgacgcttt
     2461 ccaagcgatt gtcgggttca acctcatagc gatatgagga atgtggttct gtgtcgaaat
     2521 ttctcaggcg aggcactcgt gtctcgttcg cttcgaacaa gagagatacg caccaaacca
     2581 tccgacggca gcaccggaag cggagcacct ggacgggtag acgagtgggc aaacgaggcg
     2641 gcgttgcgac tgaacgattt tgatgttcgg gcgttgctgg ctgaatttga aacagacgtg
     2701 ggatgacaac ttgccgtgcg caatcacggt gagatgggga ggttgcatgc tttacggaat
     2761 cagcgcgata ttcaatcaaa tgtccttttc caatgatgcc gtattggacg ccttagacgc
     2821 cggcacacgg ccacccgcgc gaactccgga tacaccgtcg agtacgcgct gacctcgaga
     2881 aagcttcact tccagtcctt gatctgacca gcggcggttt cgccgtgcgg ccggtctatg
     2941 gctgaagatg aagccccgct tgaggtcgcg ccgttggcag gaaacctgca cggcgttttc
     3001 gctaggcaac cttactcttt cggaaaagtt ttcaggcaat gacaaatacg gaacaagatc
     3061 gctggtcacg cgtgaagggt cggctgcgct cgagcgtggg ggaggacgtc tacaccagct
     3121 ggtttgcacg aatggacctg gaaagcgtcg agcaggagag cgtccgcctt tcggttccaa
     3181 cgcggttcct caagagctgg atccaggcgc attatgccga gcgcgttctc acctgctggc
     3241 aggccgaaat gccggaagta caccgggtcg accttaccgt gcgcacggcg atgcgatgcg
     3301 cggcgcccgc caaagaggcg tcgcctgccc cgctcgatgc gcgccgcacc gagcgatccg
     3361 atggccgtgc cgcgaccgag ttgcgcgcga tcgcgaccgc gccggtatcc gccagccatg
     3421 acgcgctcgg cggctcgccg ctcgacccgc gcctgacctt cgggagtttc gttatcggcc
     3481 gctccaacac cttggcgcac gccgcggcgc gccaggtggc ggaaggccgg cgcggcgacc
     3541 ccg
//
LOCUS       DATMGX010000004         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_413732, whole genome shotgun sequence.
ACCESSION   DATMGX010000004 DATMGX010000000
VERSION     DATMGX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413732"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..570)
                     /locus_tag="VNR65_00055"
     CDS             complement(40..570)
                     /locus_tag="VNR65_00055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ17094.1"
                     /translation="MGSDLISFRTAITNDAIAVAAIHEAAWRSTYQGIIPHLHLERMI
                     ARRGPRWWQQQIKRGANVTLLLFDGVPQGYATWGEARGTWPWPAGEIFELYVSPTFQG
                     VGLGSRLFATVKKALKCEGLNHLVVWALKDNETACAFYGGLGGSVVAAAPEHYGEASL
                     TRIAFVWGVNVKSSNA"
     gene            complement(637..1149)
                     /locus_tag="VNR65_00060"
     CDS             complement(637..1149)
                     /locus_tag="VNR65_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWJ17095.1"
                     /translation="MLDAKAGLNQSGRKRKKTGSARADTIDKSVIHLLHRASQRASEI
                     FAQETRDFDLTARQYAVITTVAQHEGLSQTDLVRLTGIDRSTLADVVQRLLKRGVIQR
                     ERTMQDGRTYAVSLSAEGRELLESIKPYARRADRLVLSCFGDEDGKIATQVLARLLRK
                     VDDNPADGGE"
     gene            complement(1253..1861)
                     /locus_tag="VNR65_00065"
     CDS             complement(1253..1861)
                     /locus_tag="VNR65_00065"
                     /inference="COORDINATES: protein motif:HMM:NF001323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HWJ17096.1"
                     /translation="MEQLETTKRKFDVTPFLKLALEVGPLAVFFIANGKFGIFYATAS
                     FMAATLISLVTSRIFLKRIPVLALVTGVFVMIFGCLTIYLHDDTFIKIKPTVVNTLFA
                     LILSAGLYFRRPVLKLALGEILQMQDEGWRLLTLRWIGFFIFLAVLNEVVWRNFSTDA
                     WVSFKSFGLMPLTFFFMMFQITLIMRHQISDVSSDSSEVSTS"
     gene            complement(1877..>2106)
                     /locus_tag="VNR65_00070"
     CDS             complement(1877..>2106)
                     /locus_tag="VNR65_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWJ17097.1"
                     /translation="TTGQNALSQVEIFDKTAGVTGLVMTKLDGTARGGILVAIASKYG
                     IPVHAIGVGEGVEDLQSFDARDFARAIAGIS"
BASE COUNT          508 a          590 c          569 g          439 t
ORIGIN      
        1 gtggcttgct ggtcgagcga gccataattt tcgagaggct caggcgtttg aggatttcac
       61 gttcacgccc caaacgaaag caatgcgtgt gagggatgcc tcgccatagt gctcaggcgc
      121 tgccgcaacc acggagcctc ccaggccgcc ataaaaagcg caggcggttt cgttgtcctt
      181 caaagcccag acgacgagat ggttaaggcc ctcgcacttc aacgccttct ttaccgtggc
      241 gaaaaggcgg ctgccaaggc cgacgccttg aaaagtggga ctgacgtaaa gctcgaaaat
      301 ctcgcccgcg ggccaaggcc aagttcccct tgcctcgccc caggtcgcat agccttgcgg
      361 cacaccatca aagaggagaa gggtaacgtt tgcgccgcgc ttgatttgct gctgccacca
      421 ccgcggaccg cgccgggcta tcatccgctc aagatgcaga tggggaatga taccttgata
      481 ggtcgaacgc caagccgcct cgtggatggc cgccaccgcg atcgcatcat ttgttattgc
      541 tgttcgaaaa ctgatgaggt ccgagcccat aatgcaactc tacatccaac aagtctggtt
      601 ggcgaggact ctcaagtcgt aaaactaacg ctgactctat tcaccgccat ctgctgggtt
      661 atcatcaact ttacggagaa gtctggcaag gacctgagta gctattttgc catcctcgtc
      721 gccgaagcag gacagcacga gccggtcggc acgccgagca taaggcttta tcgattcaag
      781 aagttcgcgc ccttctgccg acaggctgac cgcataggtc cgcccgtctt gcatcgtgcg
      841 ctcacgttga atgacgccgc gctttaagag tctctgcacg acatcggcaa gtgtggaccg
      901 gtcgattcca gtgagccgca caaggtcggt ctggctgagc ccctcatgct gggcaaccgt
      961 tgtgatcacc gcatattgcc gggcagtgag atcgaagtcc ctggtctcct gggcgaaaat
     1021 ctctgatgcg cgctgacttg cgcggtgcag gagatgaata acagatttgt ctatggtgtc
     1081 agcgcgcgcg cttccagttt tcttacgctt ccgccccgat tggtttagcc ctgcttttgc
     1141 gtccagcatt ctcgcccggc tgctcggtta aactctgaac attactacga aaatgaatac
     1201 gggaaccgtt aggttccatg cagagcacgg aaagattcgg aagaccctcc ctctaggagg
     1261 tagagacctc tgacgagtcc gaggatacat cggaaatttg atggcgcata atcaacgtga
     1321 tttgaaacat catgaaaaag aaggtcaagg gcatcaggcc aaacgacttg aagctgaccc
     1381 aagcgtccgt cgagaaattt cgccagacga cctcgttcag gacggccaga aaaataaaga
     1441 agccgatcca ccgcaaagtc agaaggcgcc agccttcgtc ctgcatttgc aggatctcgc
     1501 cgagggcaag tttcaaaaca ggcctgcgga aataaaggcc ggcagacagg atcaaggcga
     1561 aaagcgtgtt cacgaccgtt ggcttgatct tgatgaaggt gtcatcgtgc aggtaaatcg
     1621 tcaggcagcc gaagatcatg acgaaaacgc ccgtcaccag ggccaagacc ggaatacgct
     1681 tgaggaagat acggctggtg acaagcgaga tcaaagtggc cgccatgaac gatgcggtcg
     1741 cgtagaagat cccgaatttg ccattcgcga taaagaaaac cgcaagaggc cccacctcga
     1801 gcgcaagctt tagaaacgga gtaacatcaa acttcctttt ggttgtctca agctgctcca
     1861 cataaacctc ctgaactcaa cttatgccgg caatggcacg tgcgaaatct ctcgcatcga
     1921 atgattgcag atcttccacg ccttcaccaa caccgattgc gtgaacgggt atgccgtatt
     1981 tgcttgcgat ggcgacgagg ataccgcccc tcgccgttcc atcgagcttg gtcatgacaa
     2041 gcccggttac cccggctgtc ttgtcgaaga tttcaacctg cgaaagcgcg ttctgcccgg
     2101 ttgtgg
//
LOCUS       DATMGX010000005         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_31228, whole genome shotgun sequence.
ACCESSION   DATMGX010000005 DATMGX010000000
VERSION     DATMGX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31228"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VNR65_00075"
     CDS             <1..567
                     /locus_tag="VNR65_00075"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17098.1"
                     /translation="FEPLAVMTHASLIGTSEALHPQGSLEALATLFDFSKISRAPARF
                     DPEELKGLNAKLLHTTPYEAVIERLRARGIDDGEAFWLAVRANLEVFADVDRWWTVVR
                     GPVTPMIEDAAFLDQALVHLPPEPWDAGTWRNWTETLKQATGAKGRALYHPLRLALTG
                     LERGPELKDLLPLIGHEKAAARLSGKTC"
     gene            714..1286
                     /locus_tag="VNR65_00080"
     CDS             714..1286
                     /locus_tag="VNR65_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HWJ17099.1"
                     /translation="MSPDQLAALRAFFATSEDRLPDIVALMYDRFLRAMPDTAPLFKG
                     NMQEQHRQFTLMLWSIVRLTRSSELWPVNAWTGQAPIPAVEKLGMRHAHIGVRPEHFE
                     TMKMVLSRCFRDLFPKDFTPSVEKALAFIFDVLSHATEGSKANSRRSRMTQKAVANRS
                     FVGELRPRHSFSEGLQDLEASPLRRHLKLT"
     gene            1683..>2202
                     /locus_tag="VNR65_00085"
     CDS             1683..>2202
                     /locus_tag="VNR65_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17100.1"
                     /translation="MDNLPPDLVRQWKTPDGRARLSATPKGNANNTEVLQRFVDAVLK
                     VEPTATGEAVGIQKAGETVVSAFIEAGLWALLSISILLWLFLRRVSDVGLTLFPLILA
                     AMVTLEICSAIGFKLNFANIIALPVLLGLGVAFKIYYIVAWRQGQTDLLASPLTRAVF
                     FSGLTTAVAFGSL"
BASE COUNT          443 a          640 c          636 g          483 t
ORIGIN      
        1 ttcgagccgc tggccgtgat gacacacgcc tccctgattg gcacgtccga agctcttcac
       61 ccgcagggga gcctcgaagc attggccacc ctcttcgact tttcaaagat ctcccgcgct
      121 ccagcgcggt tcgatcccga agaactgaag gggctgaatg cgaaactgct ccacaccacg
      181 ccgtatgaag cagttatcga gcgccttcgc gcacgcggca tcgacgacgg cgaagccttc
      241 tggcttgcag tccgcgccaa cttggaagtt ttcgccgacg tggaccggtg gtggaccgtc
      301 gtccgagggc cggtcacgcc catgatcgaa gacgccgcct tcctggatca ggcgcttgtg
      361 catttgccgc cagagccgtg ggatgcaggg acgtggcgga actggactga aaccctgaaa
      421 caggccacgg gcgcaaaagg acgcgcgctt taccaccccc tgcgtctggc cctgacgggg
      481 ctcgagcgcg gcccggaact gaaggacctg ttgccgctca tcggccatga gaaggccgcc
      541 gccaggctga gcgggaaaac ttgttgagga tgcacctact gcgttcgcca gacaatttga
      601 caattcgtaa agcttaggct ctgatgatca attgggtcta aagattttgc ttatcgggct
      661 gtttccaccg cgttgctggc cgcagccatt ttgtgcagcg tggagttatt gcgatgagcc
      721 cggatcagct tgcggcattg agggccttct tcgccacttc agaggaccgg ctacccgaca
      781 tcgttgccct tatgtatgac aggttcttaa gagcgatgcc ggacacagca ccgctcttca
      841 agggcaacat gcaagagcaa cacaggcagt tcacgcttat gctctggagc atcgtcaggc
      901 ttacgcggtc gagcgagctt tggcccgtca acgcatggac cggccaggcg ccaattcctg
      961 cggtcgagaa gcttggaatg cggcacgctc acatcggggt gcggcctgaa cattttgaaa
     1021 ccatgaaaat ggttctgtcc cgatgcttcc gcgacttgtt tccgaaggat ttcacgccct
     1081 ccgtggaaaa ggccctcgct ttcatcttcg atgttttgtc gcacgcgaca gagggctcga
     1141 aagccaacag ccgtagaagc cgtatgacgc aaaaagccgt tgcaaacaga agtttcgtgg
     1201 gcgagctgcg gccaagacac agcttttctg aaggcctgca agacctggag gctagccctt
     1261 tgcgacgcca tttgaaactt acgtaatacg aaccgccata agtcacgacg cctttgccca
     1321 tggccgcctt tggaggcaag aaaaagtatt tatatgttta cttatggccg ttttacaaaa
     1381 cgaaaccaat tttagccgat tgttaactct cacaggctga cgcgcctgga gaagctgcat
     1441 ggatgatatg agtcgagcga tctgacggtt gcttaagttg cggtatcgcg ccgccggtca
     1501 cgaaccagtt cgttcatccc cacgatcttc ggcaatttcc tgataccgcc catgatcggc
     1561 gcaagggatt tggccttccc gcgccttaat ctcatcagct ggtctggcca atcatcgtgt
     1621 aacccgcctg ggttaagatc ggtagccgcc cttgcagaag gcctggacgg aaacgcattg
     1681 cgatggacaa tctcccgccg gacctcgttc gacaatggaa gacaccggac gggagggcgc
     1741 ggctttcggc gacgccgaaa ggcaatgcga acaacaccga agtgctgcaa cggttcgtcg
     1801 atgcggtttt gaaggtcgag cccacagcga ctggcgaagc tgtcggcatt cagaaggcgg
     1861 gcgagacggt ggtcagcgcc ttcatcgagg cggggctttg ggcgctgctt tccatttcga
     1921 tcctgctctg gctcttcctg cgcagggtat cggatgtggg gctcacgctc tttccgctca
     1981 tcctcgccgc catggtgacg ctcgagattt gctctgcaat cggcttcaag ctgaatttcg
     2041 cgaatatcat cgcgttgccg gtcctgctgg gtctgggcgt cgctttcaag atttattaca
     2101 tcgtcgcctg gcgtcagggc caaacggatc tcctggcttc gcccctgact cgcgcggtgt
     2161 tcttcagcgg tttgaccacg gcggtggctt tcggcagcct gt
//
LOCUS       DATMGX010000006         2706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_377081, whole genome shotgun sequence.
ACCESSION   DATMGX010000006 DATMGX010000000
VERSION     DATMGX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377081"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VNR65_00090"
     CDS             <1..802
                     /locus_tag="VNR65_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ17101.1"
                     /translation="ATDDRLREEDCRANVLHVAPTRSMLADGLAQYLVLKTWRKWFFV
                     VGSHPKDKLWGDALKRSATRFGAKVVEERTFADTGGARQTDSGIAQIQRQIPTFTQGA
                     PDYDVLVTADESEVFAAYFPYRAYAPRPVAGSSGLVPTSWEPSMEQWGATQLQNRFMN
                     KVSRRMTALDMQAWSAARMIGEAVTRTKSADMQAVRTYLLSPDFALAAFKGQKLTLRP
                     WNLQLRQPILLSDGRNITSISPQEGFLHQVSELDTLGYDQPETKCALK"
     gene            820..1956
                     /locus_tag="VNR65_00095"
     CDS             820..1956
                     /locus_tag="VNR65_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HWJ17102.1"
                     /translation="MTPALAEPLLKSWPGTTRPSSVTMEVPTVSLDGRLKGGHDGREP
                     GKPSGGFGMMRHAARQMILAMAVLGLSAHAAFGVEAYISNEKSNTVSVLDTDKMEVTN
                     TIKVGQRPRGIVLSKDGKFVAVAAGDDSTIQIIDTASKEVTGTLPSGPDPELLAFSPD
                     GKTLYVANENDNTVTMVDVPSRAAIGEVPVGVEPEGMGISPDGKTVVCTSETSNMAHF
                     IDIQSKDIVANVLVDARPRFAEFKKDGSELWVSAEVGGTVSIIDPIKHAVTKKITFDV
                     PGLRREAIQPVGINLTKNGKLGFVMLGPANRVAVIDGNTHEVLKYLLVGQRVWHGAFT
                     PDEKYLLVTNGISNDVSVIDVASLKVIKSIQVGELPWGVTISFQ"
     gene            2043..>2706
                     /locus_tag="VNR65_00100"
     CDS             2043..>2706
                     /locus_tag="VNR65_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ17103.1"
                     /translation="MSHSYGQRKALDDVSITVAPSTFAVLLGLNGAGKSTLFSLITRL
                     FNAQSGEIRIFGHDLHRESGAALSLLGVVFQARTLDLDLSLAQNLRYHAALHGIGGRE
                     ARLLSQRLLEHIGLEARAGDKARDLSGGQMRRVEIVRALLHKPKLLLLDEPTVGLDIE
                     ARADILRHVRSLVSTEGLSTLWATHLIDEVAETDTVIVLHRGKVLAQGIAADIVRSVG
                     ARD"
BASE COUNT          541 a          794 c          835 g          536 t
ORIGIN      
        1 cgcgacagac gacaggctga gagaagagga ttgccgcgcc aacgtccttc acgtcgcgcc
       61 cacgcgctcg atgctggcag atgggctcgc gcaatatctt gtcttgaaga cctggcgcaa
      121 gtggttcttc gtcgttggtt cgcatccaaa ggacaagctt tggggcgacg cgctgaagag
      181 atccgcgaca cgtttcgggg ctaaggtcgt ggaagagcga accttcgccg acacaggcgg
      241 cgcgaggcag acggacagcg gcatcgcgca aatccagcgc cagattccga ccttcacgca
      301 aggtgcgccg gattacgatg tgctcgtgac tgctgacgaa agcgaggttt tcgcggccta
      361 ctttccttac cgggcttatg cgccccgccc agtggcgggc tcttccggcc ttgttccgac
      421 gagttgggag ccgtccatgg agcagtgggg cgcgacccag ctgcagaacc gcttcatgaa
      481 caaggtctcg cggcgcatga cggcactcga catgcaggcc tggtcggcgg cgcgaatgat
      541 cggtgaagcg gtgacgcgca caaagtcggc ggacatgcag gctgttcgca cctatcttct
      601 cagccccgat tttgcgttgg cggccttcaa aggccagaag ctgacgcttc gcccctggaa
      661 cttgcaactg cgccagccca tacttttgag cgacgggcgg aacattacgt cgatttctcc
      721 gcaggaaggt ttcctgcatc aggtgtcaga gttggatacg ctcggctacg atcagccgga
      781 aacgaagtgc gcgcttaaat agggcggaga gggaagggca tgacaccggc tttggctgaa
      841 ccgctgctta agtcatggcc gggcacgacc cggccatcca gtgttacaat ggaggtgccg
      901 actgtttccc tggatggccg cctcaagggc ggccatgacg gtcgggagcc cggcaagccg
      961 tcaggcggat ttggcatgat gcggcacgcc gcccgccaaa tgatcctcgc gatggcggtg
     1021 ctggggcttt cggctcatgc tgctttcggc gttgaagcct atatttcgaa cgagaagagc
     1081 aatacggttt ctgtgctcga taccgacaag atggaagtca cgaacacgat caaggtgggc
     1141 cagcgtccgc gcggcatcgt actgagcaag gacggtaagt ttgtcgccgt cgccgctgga
     1201 gacgacagca ccatccagat catcgacacc gcgagcaagg aggtgacagg cacgctgcca
     1261 tccggccccg accccgagct tttggctttt tctccggatg gcaagacgct ctatgtcgcc
     1321 aatgagaacg acaataccgt aacgatggtc gatgtgccca gccgcgcagc gataggcgag
     1381 gtgccggttg gggtcgagcc tgagggcatg ggcatcagcc ccgacggcaa gaccgtggtc
     1441 tgcacctccg agacgagcaa catggcgcac ttcatcgaca tccaaagcaa ggacattgtc
     1501 gccaatgtgc tcgtcgatgc taggccgcgc tttgccgagt tcaagaagga cggatcggaa
     1561 ctctgggtct ccgctgaggt tggtgggacg gtcagcatca tcgatcccat caagcatgcc
     1621 gtgaccaaga aaataacttt cgatgtcccc gggcttaggc gtgaggccat tcagcccgtc
     1681 ggcatcaacc ttaccaagaa cgggaagctc ggttttgtca tgcttggccc ggcgaaccgc
     1741 gtcgcggtca tagacggcaa tacgcatgag gtgcttaagt acctgctcgt tggccagcgg
     1801 gtctggcatg gcgctttcac gcccgatgag aaatatctgc tggttacgaa cgggatttcg
     1861 aacgatgttt ccgtgatcga tgtcgcaagc ctcaaggtga tcaagtcaat tcaggttggc
     1921 gaactgccct ggggcgtcac catatcattt caatgatgga cactttagcc gacgcaacac
     1981 tgctagaccc ttcgccaagg atcgcacaag gcgacgtagg gcccgctctc gaaatagcgc
     2041 gcttgagcca ctcctacggg cagcgcaagg cactggatga tgtctcgatc accgtggccc
     2101 cctccacatt cgcggtgctc cttgggttga acggcgccgg gaaaagcaca ctattttcgc
     2161 tcattacgcg ccttttcaat gcgcaatcag gcgagatccg catctttggc cacgatcttc
     2221 accgcgagtc gggcgcggcg ctgagccttc tcggcgttgt ttttcaggcg cggacactcg
     2281 atctcgatct ttcgctggcg cagaaccttc gctatcacgc cgcgttgcat gggattggcg
     2341 gccgcgaagc ccggcttctc agccagcggc ttctggagca cattgggctg gaggcgcgtg
     2401 ccggcgacaa ggcgcgcgat ctttcaggcg gccagatgcg gcgtgtggaa atcgtccggg
     2461 ccttgctgca taagcccaag cttcttctgc tcgacgaacc gacagtgggc ctcgatatcg
     2521 aggcgcgcgc cgatattctg cggcacgtcc gcagcctagt ctcgaccgag gggctcagca
     2581 cgctctgggc cacgcatctg atcgacgagg tagcggaaac cgatactgtt atcgtcctgc
     2641 atcgcggcaa agttttggct caaggcatcg ccgccgacat cgtcagatct gttggggccc
     2701 gcgata
//
LOCUS       DATMGX010000007         6347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_30794, whole genome shotgun sequence.
ACCESSION   DATMGX010000007 DATMGX010000000
VERSION     DATMGX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30794"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VNR65_00105"
     CDS             complement(<1..287)
                     /locus_tag="VNR65_00105"
                     /inference="COORDINATES: protein motif:HMM:NF023682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HWJ17104.1"
                     /translation="MIIVEIPPDHPLRPAVSRLIRATYYLIYDARIKALPKTLVALVD
                     DKGKVLAAAGLRDASEPFFSEHYLDLPIQRLLSQVSRKPLERKSIVEVSCLA"
     gene            complement(384..1337)
                     /locus_tag="VNR65_00110"
     CDS             complement(384..1337)
                     /locus_tag="VNR65_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWJ17105.1"
                     /translation="MSKSSYRIAVNVEWTSKCNARCAMCPRTMISDPKLMTLSTWEQV
                     LSRLTYEDVFRVIIAGYGEPTTHPKFDDFVDAMHGRHLQFDMATNGSMLDKERLERMD
                     GLFTKLMISFSSTDPEVYQAVHTNLDQQQVMENILLAHRTLKKTQLIINLSPTPECLM
                     TLDGTIQWFHQNGIRDLHMSPTYYDRAGAMNTRGMPSEADLRAKIKFYRLTSQEMGFI
                     SGVGDFARQWRSNRFKCIPRNTNMLINAQGHYTFCFNDIRHSHEIGHVASMSLRQALA
                     VRESKGADSAICDNCNLRGRYRPRELTRIAFAYAKTRMAAA"
     gene            complement(1650..3032)
                     /locus_tag="VNR65_00115"
     CDS             complement(1650..3032)
                     /locus_tag="VNR65_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ17106.1"
                     /translation="MRRYSRSLWFRLIFGLSMGSLAAVLVASTFLYVRFKNVSAESRE
                     RTLQGQAKLIAKLFQSAGGRELILPDEIAPYYGNGAGKFAVLFEDETFLTGSAGVSHA
                     FHPIDREVVREFFSYAQPDGKPAYHGISQLIKGSSPPIWVQVAFADNEVIFDSVLEEF
                     VIDIAWIWLPFVVILLMINMIVIRVSLKPLVQASEQAAQIGPMAVSKRISEKGLPREV
                     LTLVRAINHALDRLEEGYREQGAFIADAAHELRTPIAIMTTHMDILPEFEGKAALKEE
                     LGGLKRLVNQLLDNARIEALRIAPNEAADLNALALDVAAFLAPCAIARGKTIEVSSAR
                     DPALVNGSYDYLFRALRNLVENAIEHTPAGTAVHIAIRSPSTLVVSDCGPGIPPAERE
                     AIFERFWQGRRDRGGGAGLGMAIISRTVAAHKGTIEVGDREGGGAKFTVYFPPVAHAG
                     AAFPAEAPSA"
     gene            complement(3029..3715)
                     /locus_tag="VNR65_00120"
     CDS             complement(3029..3715)
                     /locus_tag="VNR65_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ17107.1"
                     /translation="MRLLIAEDNARMGQYLRTALSDRGFTVDLVETAADAEAALFGVD
                     YNALILDLGLPDADGMSLLKSMREKSDMRPVLILTARDSTKTLVDSLNSGADDYLCKP
                     FEMDELVARLRALLRRPGQRKSVVIAEGNIRLDTVEREVRIDGTVIEVPRRELYALEL
                     LLRRTGSVISKSTMESMLYSFGEEIASNAVEVVIHRLRKRLLDARASVAIHTIRGVGY
                     MLLVEATESA"
     gene            3767..4189
                     /locus_tag="VNR65_00125"
     CDS             3767..4189
                     /locus_tag="VNR65_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17108.1"
                     /translation="MKATTSLSVAIMSKARRIRGRWPGVMHLSWLRRIVSGHAFGKAA
                     GDNKEVNKLGAPQTGTSRATFLETIKGTKFADSPAIYFIVYDYDGVAVYHPDPARQGR
                     NRSDAAADIVLISSSKTPTPFLKRLAYKRSAFNQLIIL"
     gene            4470..5732
                     /locus_tag="VNR65_00130"
     CDS             4470..5732
                     /locus_tag="VNR65_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17109.1"
                     /translation="MAQQMTFAEIGTLYNSISNELEGGVTQKNQTQLVKQVSTVQTQL
                     QSLIDSGALSNLDGAGNPAVSVVHAQNVADQMGFLKAQIGTFGTNAFTPKFINDVVRD
                     VQDIVAGDTALTALAHQNNHSGFQQVSFLLTPPTPFPDSGVQTTTLLQFVSDSNSLAA
                     RAQALAGADPNSAAAQQLITDIHTFSANADAYSSAQGGLFSARFNNEFTSNGVQGTAS
                     RELITGLQTGNADLVNGAASVLAANGGDVRMNMLATGDTFTSEPKGGIPANIQTVHDA
                     GVVFNDAMTKLIGGVYSGNQTSIVNDLNATKMGLQNAVTAQGLTDHALQNAEKVMNLL
                     DKEAALVGGIDTANPTQISAVNGQIGKVQSQILNTVNHDATLSAQAVGTDGTTGFVAL
                     PSGTTPVDHGLGHNDLSAAAHHFGHMWG"
BASE COUNT         1409 a         1800 c         1794 g         1344 t
ORIGIN      
        1 gcaagacaag aaacttcgac gattgacttg cgctccagag gcttgcggga aacctgactc
       61 aggagcctct gtatcggcag atcgaggtaa tgctcggaga aaaagggctc cgaagcgtcg
      121 cgcagaccgg cggcggcgag aacctttccc ttatcatcga ccaaagcgac aagggtcttc
      181 ggcagagctt tgatcctggc gtcgtatatc aaatagtagg ttgcgcgaat aagccgggaa
      241 acggctggcc gcaacggatg gtctggcggt atttcaacta tgatcattct tcatcgaacc
      301 tggcatgagt gtgtatctgt ccacttttca aggattgcac ctctcgatgc acaatctcct
      361 gggtggcgct cgctgggcat cagttatgcg gccgccatgc gggtcttcgc ataggcgaag
      421 gcaatccggg tcaactccct cggccggtag cgcccgcgca aattgcagtt gtcgcagatc
      481 gctgaatccg cgcccttcga ttcccgcacc gccagggcct gcctaagaga catgctcgcc
      541 acatggccga tctcgtggct gtgccggata tcgttgaagc agaaggtgta atggccttgc
      601 gcgttgatga gcatattggt gttgcgcgga atgcacttga agcggttgga ccgccattgg
      661 cgtgcgaaat cgcccacgcc tgaaatgaag cccatctcct ggctggtcag gcgatagaac
      721 ttaatcttcg ccctcagatc ggcttcggaa ggcatgcccc gcgtattcat ggcgcccgcc
      781 cggtcgtaat aggtcgggga catgtgaaga tcgcggatgc cgttctgatg gaaccattga
      841 atggtaccgt cgagtgtcat gaggcattca ggcgtcggcg acaagttgat gatgagctgc
      901 gttttcttca gcgtccgatg agcgagcagg atgttctcca tcacctgctg ctgatcgaga
      961 ttggtatgca cggcctgata cacctcgggg tcggtgctgg aaaacgagat catcagcttg
     1021 gtgaagagcc cgtccatgcg ttcgaggcgc tctttgtcga gcatactgcc gttggtcgcc
     1081 atgtcgaact ggagatggcg gccgtgcatc gcgtcgacaa aatcgtcgaa cttgggatgg
     1141 gtcgtgggct cgccatagcc ggcgatgatg acgcgaaaga catcctcgta ggtgagtctg
     1201 gacaggacct gctcccaggt cgaaagcgtc atcaacttcg ggtctgaaat catggtgcgc
     1261 gggcacatgg cgcagcgcgc gttacactta ctcgtccact cgacattgac tgcaatgcgg
     1321 tacgaggatt tggacatacg ggcttccccc aatcaatttg gcttttccgt tcgcggttcg
     1381 gaccgccgcc acaaactaaa gttgtgttta gaatggctgg aaattagtca caagtatggc
     1441 tatacttata cacatcaata atttttatta caaaggctcc gctaaaagct gtaaaatgat
     1501 gtatcgtcaa aatatagaca caaaaaaacg cgccggaaaa gctagtgggc tttttcgacg
     1561 ctaaacttta tgtgcgcgtg cactctatga atgcccgctt acggaatgct tacggctcta
     1621 attattttca tattggccga gcctgcgctt caagcggacg gagcttccgc cgggaaggcg
     1681 gctccggcat gagcgacggg cgggaaatac acggtgaatt tcgcaccgcc gccctcgcgg
     1741 tcgccaacct cgatcgtccc tttgtgcgcg gcaaccgtgc gcgagatgat cgccatgcca
     1801 agacccgcgc cgccgccacg gtcccggcgg ccctgccaga agcgctcgaa aatcgcctcg
     1861 cgctccgcag gcggtatgcc aggcccgcaa tccgaaacca cgagcgtgga cgggctcctg
     1921 atggcgatat gaacggcggt tccggcgggc gtgtgctcga tcgcgttttc cacgagattt
     1981 cgcagcgcgc ggaagaggta atcgtaggag ccgttcacga gagcgggatc tcgcgcgctc
     2041 gacacctcga tggttttccc ccgcgcgatg gcgcaaggcg cgagaaacgc cgccacgtcg
     2101 agcgccagcg cgttcagatc cgcagcctcg ttcggtgcga ttctcaacgc ttcgatccgc
     2161 gcattgtcga gaagctgatt gacgagcctt ttcagcccgc caagttcttc cttgagcgcg
     2221 gccttgcctt cgaactctgg aaggatatcc atatgcgtcg tcatgattgc tatgggcgtg
     2281 cgcagttcgt gggccgcgtc ggcgatgaag gcgccctgtt cccgatagcc ctcctccagc
     2341 ctgtcgagag cgtggttgat ggcgcgaacc agcgtcagaa cctcgcgcgg caagcccttc
     2401 tcggagatac gcttcgacac cgccatgggg ccgatctggg cggcctgttc cgaggcttgc
     2461 accagcggct tcaggcttac cctgattacg atcatgttga tcatcaggag aattacgaca
     2521 aagggcagcc aaatccaggc gatgtcgatc acgaactctt cgagtacgga gtcgaaaatc
     2581 acctcgttgt cggcgaacgc gacctgcacc cagataggcg gcgaggagcc ctttatcagc
     2641 tgggatatgc cgtgataggc aggtttgcca tctggctggg cataagagaa aaactcgcga
     2701 accacttcgc ggtcgatggg atgaaacgcg tgggacacgc ccgccgaccc ggtaagaaag
     2761 gtctcgtcct cgaagagaac tgcgaacttg cctgcgccgt ttccgtaata gggcgcgatc
     2821 tcgtccggca agataagctc gcgcccgccg gccgactgga agagcttggc gatcagcttg
     2881 gcctgcccct gaagcgtgcg ctcacgcgac tccgcgctga cattcttgaa gcggacataa
     2941 agaaaggtgc tggccacaag cactgcggca agcgagccca tggagaggcc aaaaatgagg
     3001 cggaaccaga gagaccggga ataccgcctc atgcgctctc ggtggcctcg acgagaagca
     3061 tatagccgac gccccggatg gtatggatcg ccacgctggc gcgggcgtcc agaaggcgct
     3121 tgcgcaagcg gtggatgaca acctcgacgg cgttcgaggc gatctcctcg ccgaaggagt
     3181 aaagcatgct ttccatggtt gacttggaga taacgctccc ggtgcgccgc aaaagcagct
     3241 caagggcgta aagctcgcgg cgcggaacct cgatcaccgt cccgtcgatg cggacttccc
     3301 gctcgacggt gtcgagccga atgttgcctt cggcaatcac gacgcttttc ctttggccgg
     3361 gcctgcgcaa gagagcccga agacgcgcga caagctcatc catttcgaag ggtttgcaga
     3421 gatagtcgtc agcgccgctg ttaaggctat ccacgagcgt cttcgtgcta tcacgagcgg
     3481 tgaggatcag gaccggacgc atgtcgcttt tctcgcgcat ggatttcagc agcgacatgc
     3541 cgtctgcgtc gggcaggccc aggtccaaaa tcaacgcatt gtagtcaacg ccgaaaaggg
     3601 ccgcttcggc gtcggcggca gtctcgacca gatcaaccgt aaaccccctg tcactgagcg
     3661 cggtgcggag gtactgaccc atacgggcat tatcttcggc gatgagaagt ctcatggtcc
     3721 ttatctcgcc caggagccgg agcgcaccct caggctgttt ccatcgatga aagcaaccac
     3781 ttcgctatca gtagcaatca tgtcaaaagc gaggagaatt cggggacgct ggccaggggt
     3841 tatgcatctt tcatggctga ggcgcatcgt tagcggacac gccttcggta aggcggcggg
     3901 agacaataag gaagtgaaca agttaggggc gccacaaacc gggaccagcc gggccacctt
     3961 tcttgagacg atcaagggca cgaaatttgc cgacagccct gccatttatt tcattgttta
     4021 cgattatgac ggcgtcgccg tctaccatcc cgatcctgca aggcaggggc gaaaccgatc
     4081 cgatgccgcc gctgatatag tcttaatcag ctccagcaaa acgcccacac cctttctaaa
     4141 gagactggca tataaaagat cggctttcaa tcaactgatt attttataag ctttcaataa
     4201 gttccactaa catgggtgat caatcctttg tgaatacagt catcgcgcgc gagtgcctta
     4261 gatatacctt aatattctcg cgttcttgtc ccactgcctt tgtatagtga tgaagatcgt
     4321 tgcagagcgg acgttggtga gaggttcctg atcgccccac agccccatca ataaacagcg
     4381 tggccttcga cgactccacc agggcgatca tggggccgcc ctatcgaagc gtttcacgtg
     4441 caaggacacg gcgaacgagg aggaacgaca tggctcaaca aatgacgttt gcggaaatcg
     4501 gaacccttta caattcgata tccaatgaat tggaaggtgg tgttacgcaa aaaaatcaga
     4561 cccaactcgt caagcaagtc tcaactgtcc aaacccagct ccaaagtctc attgatagcg
     4621 gtgccttgag caatctggat ggtgcgggca atccggctgt aagcgtcgtc catgcccaga
     4681 acgttgccga tcaaatgggc ttcttgaaag cacagatcgg cacctttgga acgaacgcct
     4741 ttaccccgaa gttcatcaac gacgtcgtca gggatgtcca ggacatcgtg gctggcgaca
     4801 cggccctgac cgcacttgct catcagaaca atcattccgg gttccaacaa gtttcctttc
     4861 tgctgacgcc tccaacgcct ttcccagatt cgggtgtgca gacaactacc ctccttcagt
     4921 ttgtctcaga ctcgaattcg ctcgcggcac gtgcgcaagc gcttgcgggt gccgatccga
     4981 actcggcagc ggcccagcaa ctgataaccg acatccatac gttctccgcg aatgcggatg
     5041 cttattcgag cgctcagggt gggttgttct ccgctcgctt caacaatgaa ttcacgtcca
     5101 atggtgtcca gggaacggca tcgcgggaac tgatcaccgg cctgcaaacc ggcaacgcgg
     5161 atctcgtcaa tggcgccgca tccgtcctcg cggcaaacgg cggcgacgtg cgcatgaaca
     5221 tgcttgcaac aggagatact tttacgtctg aaccgaaggg cggcattccc gccaacatcc
     5281 aaacggtgca cgatgccgga gtcgtgttca atgatgccat gaccaagctc attggcggcg
     5341 tctattccgg gaaccagacg tccatcgtca acgatttgaa tgcgaccaag atgggacttc
     5401 agaatgcggt cacggcgcag gggcttaccg atcacgccct gcagaatgcc gagaaggtca
     5461 tgaacctgct ggacaaggaa gccgcgctcg ttggcgggat cgacaccgca aaccccaccc
     5521 agatctcggc ggtcaatggc cagatcggca aggtgcagtc gcaaattttg aatactgtga
     5581 atcatgacgc aaccctgtcg gctcaggccg ttggaactga cggtaccacc ggtttcgtgg
     5641 ccttgccctc agggacaact ccagtggatc acggtctcgg ccataatgac ctctctgcgg
     5701 ccgctcacca ttttgggcat atgtggggtt gaaccgaaat ccttaagggc gcccaggctt
     5761 gctggcaatt tgtcagcaag ccgcttcctg gggtaaaaag ccgttcgatg cgcgctgggt
     5821 aacggccagc tcacgggagc tcaccaacaa gttgcaccgc ggccattatt gaggctccca
     5881 taccattacg gtggcatcgt tcccatcctt tagccgcgtc actgacatgg cccaccttcg
     5941 cctttttctt gaagtccgag ctctgaaagc gactgttcac ggcatccgtt tcggcaaggc
     6001 gatgccttcg agacgctgaa aatcctattg ctaacagtca ctttacggct tcacatagcc
     6061 tctctaaaaa gattctaagt tattgtcgga acggggtttt tcttgaccgc tgtcacggta
     6121 tggctcagat tgcaacctga agtcgatttc cagagagcat taatgtttca cgatcatcgc
     6181 acccgtacac aggagtgtgc aattttgctt tgaacgtcta aacgcggcta tcgggccgcg
     6241 tgcaggccat caaggtattg ccgcatcata tagaatggca gaggtgaggc tcaaggtttg
     6301 ctctagcgcg gcgtgcgcaa aatcggaccc ggttgcgact cttaacg
//
LOCUS       DATMGX010000008         3086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_29684, whole genome shotgun sequence.
ACCESSION   DATMGX010000008 DATMGX010000000
VERSION     DATMGX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29684"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /gene="kdpB"
                     /locus_tag="VNR65_00135"
     CDS             <1..1172
                     /gene="kdpB"
                     /locus_tag="VNR65_00135"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HWJ17110.1"
                     /translation="GDVDTLLLDKTGTITLGNRQASEFIPLRDVTEKELADAAQLASL
                     SDETPEGRSIVVLAKDRYGIRGRDMAQFSGHHFIPFSAQTRLSGIDLEGEQIRKGAVD
                     AVINYVRQVTGNPNMPAPPELERAAEAISTAGGTPLAVARNGRLLGVVYLKDIVKGGI
                     RERFAELRRMGIRTIMITGDNPLTAAAIAAEAGVDDFLAQATPENKLNLIREEQAKGK
                     LVAMCGDGTNDAPALAQADVGVAMNTGTMAAREAGNMIDLDSDPTKLIEIVEIGKQLL
                     ITRGALTTFSIANDVAKYFAIIPAMFVAFYPQLNALNVMGLSTPQSAILSAIIFNALI
                     IIFLIPLALRGVRYRPLGASKMLTRNLLIYGVGGIIVPFIGIKLIDMVVSAAGLA"
     gene            1284..1898
                     /locus_tag="VNR65_00140"
     CDS             1284..1898
                     /locus_tag="VNR65_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HWJ17111.1"
                     /translation="MLRQIQPAIVMIILLTVITGLAYPLGMTGLAQLIFPHQANGSLI
                     EKDGKVIGSELIGQNFADARYFHGRPSATTDTDPNDPTKTVPAPYNASNSGGANAGPT
                     SKSLIDRVTQDADKLKAENPNVPVPVDLVTTSASGLDPEISPAAVFFQVPRVAQARGI
                     PEARLRELVNQSIEGRSLGILGERRVNVLKLNMRLDAEAVSANP"
     gene            1993..2475
                     /locus_tag="VNR65_00145"
     CDS             1993..2475
                     /locus_tag="VNR65_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWJ17112.1"
                     /translation="MRISDLITPASIAVRVHIDDKPDALRFAAMALGSRSGLDPERIR
                     EALAAREALGSTGIGHGVAVPHVCLPDLDRPYAFLCSLAKPIGFDSIDGERVDLVCAV
                     INPTNSQSGASESLTNLAAISRLLRDKDRASAMRKAIRPADIYDVVVRQAEMAKQPAS
                     "
     gene            2480..>3086
                     /locus_tag="VNR65_00150"
     CDS             2480..>3086
                     /locus_tag="VNR65_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HWJ17113.1"
                     /translation="MAQSDTIRRPSPDALLEETRREDRSRGRLKVFIGAAPGVGKTYE
                     MLMSARAKLAEGADIVIGVVETHGRAETQALLEGFEIIPRVKIDYHGHVLEEMDLDAI
                     LKRHAEIVLVDELAHTNAPASRHPKRYQDVEDLLAAGIDVYTTLNIQHVESLNDVVAQ
                     ITRIRVRETVPDSLLNKADDIEVIDIAPDELIQRLKEGKVYV"
BASE COUNT          654 a          956 c          893 g          583 t
ORIGIN      
        1 cgggcgacgt ggatacgctg ctcctcgaca agaccggcac catcacgctc ggaaacaggc
       61 aggcgtcgga gttcattccc ttgagggatg tcaccgaaaa agagcttgcg gatgcggcgc
      121 agcttgcctc cctttccgat gagacgccgg aagggcgctc tatcgtggtg ctggccaaag
      181 accgttacgg tatcaggggc cgcgacatgg cccagttcag cggccatcat ttcattccct
      241 tctcggcgca gacccgcctc agcggcatcg accttgaggg cgagcagatc cgcaagggcg
      301 ccgttgacgc ggtcatcaac tatgtgcgcc aggtgaccgg caatcctaac atgccggctc
      361 cgccagagct tgagcgtgca gccgaagcga tatccaccgc aggcgggaca ccgctggccg
      421 tcgcacgaaa cggccgcttg ctaggggtcg tctatctaaa agatatcgtg aagggcggca
      481 tcagggagcg cttcgccgag ctgcgccgca tgggtatccg caccatcatg atcacgggtg
      541 acaatccgct aaccgccgcc gccatcgccg ccgaagccgg ggtcgacgac ttcctggcgc
      601 aggcaacgcc cgagaacaag ctgaacctga tccgcgagga gcaggcgaag ggcaagctcg
      661 tcgccatgtg cggcgacggc acgaacgacg cccctgccct ggcacaggct gacgtgggcg
      721 tggccatgaa caccggcacc atggcggcgc gcgaagccgg caacatgatc gacctcgaca
      781 gcgatcccac gaagctgatc gagatcgtgg agatcggcaa acagctcctc attacgcgcg
      841 gggcgctcac aaccttctcc atcgccaacg acgtcgcgaa atatttcgcg atcatcccgg
      901 cgatgttcgt tgccttctat ccgcagctca acgcgctgaa cgtgatgggc ctttcgacgc
      961 cgcaaagcgc gatcctttcc gcgatcatct tcaatgcgct gatcatcatc ttcctgatcc
     1021 cgcttgcgct gcggggtgtg cgctaccgtc ctttgggagc ctcgaagatg ctgactcgca
     1081 accttctgat ctatggcgtc ggtggcatca tcgtcccatt catcggcatc aagctgatcg
     1141 acatggtcgt ttccgccgcg ggccttgctt agagcagcgc acgtccattt ggacgcagat
     1201 aagctgctct aacactttag aatcgatcaa cttttccgca tttaatctcc gattaaaggc
     1261 gggttgatct aaggagagca atcatgttga gacaaatcca acccgctatc gtcatgatta
     1321 tcctgctcac ggtcattaca ggcttggcct acccgctggg catgaccggg ctcgcgcaac
     1381 tcatctttcc tcatcaagcc aatggaagct tgatcgagaa agacggcaag gttatcggct
     1441 ccgaactgat cggccagaac ttcgccgatg cgcgctactt ccacggcagg ccttcggcca
     1501 ccacggacac cgacccgaat gacccgacaa aaacggttcc tgcgccctac aacgccagca
     1561 attcgggtgg cgccaatgcg ggaccgactt ccaagagcct gatcgaccgc gtgacccagg
     1621 atgccgataa gttgaaggcc gaaaacccga atgtgcccgt gcctgtcgat ctcgtcacca
     1681 catccgccag cggtctcgat ccggagatca gtcccgcagc ggtgtttttt caggttccac
     1741 gcgtcgcgca ggcaagaggg atccctgagg cccggctgcg cgaactcgtg aatcagagca
     1801 ttgaaggccg ctccctcgga attctcggag agcgtcgggt gaacgtgctc aagctgaaca
     1861 tgaggctgga cgccgaggcc gttagcgcaa acccttaatt gaagccagcc tacgattacc
     1921 tgaggggacg gcttgaaagt taggacatcg caccgattct caagcctagc gttccttgaa
     1981 tagggcaaga gcatgcgtat ctccgacctg ataacaccgg cgtcaatcgc cgtgcgcgtg
     2041 cacatcgacg acaagcccga tgcgctcagg tttgctgcga tggcgctcgg gtcaaggagc
     2101 ggtctcgatc ccgagcgcat ccgggaggcc ctggctgctc gtgaggcgct tggatcgacc
     2161 ggcattggcc atggcgtggc cgtgccccat gtctgcctgc ccgatctgga caggccctac
     2221 gctttcctct gttcgcttgc aaagccaatc ggtttcgatt caatcgatgg cgagcgggtt
     2281 gatttggttt gcgccgtcat caatcccaca aattcacagt cgggcgccag cgaatccctg
     2341 acgaatctcg ccgcgatctc aagactctta cgagataagg accgcgcttc ggcgatgcgt
     2401 aaagcaatca ggccggctga tatttatgat gtcgtcgtga gacaggccga gatggccaag
     2461 caaccagcaa gctaagggaa tggcccagag cgacactata cgccgacctt cgccagatgc
     2521 gctgctggaa gagacacggc gtgaggatcg aagccgcggt cgtctcaagg tgttcatagg
     2581 cgccgctccc ggcgtcggca aaacctacga gatgctgatg tccgctcgcg cgaagctggc
     2641 cgagggcgcg gacattgtca tcggtgtcgt cgaaacccat ggacgcgcgg aaacgcaggc
     2701 cctgctcgag ggcttcgaaa tcattccgcg cgtcaaaatc gactatcacg ggcatgtgct
     2761 cgaggagatg gacctcgacg ccatcctcaa gcggcatgcg gagattgtgc tggtcgatga
     2821 gcttgcccac acgaacgcac ccgccagccg tcaccccaag cgctaccagg atgttgaaga
     2881 tcttctcgct gccggcatcg atgtctacac gacgctcaac atccagcatg tcgaaagcct
     2941 gaacgatgtc gtggcccaga tcacgcgcat ccgcgtgcgc gagacggtgc cggactctct
     3001 cctcaacaag gcggatgata tcgaggtcat cgacatcgcg cccgatgagc ttattcagcg
     3061 gctgaaagag ggcaaggttt acgtcc
//
LOCUS       DATMGX010000009         2877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_374857, whole genome shotgun sequence.
ACCESSION   DATMGX010000009 DATMGX010000000
VERSION     DATMGX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374857"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VNR65_00155"
     CDS             <1..294
                     /locus_tag="VNR65_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17114.1"
                     /translation="GKIALEIVAPGREPIVFETLQDGDIVGASWLVPPYRWMFDARAV
                     GLTRAIGMDAACLRGKCEADHHLGYEMMKRFLPILVGRLHATRLQILDVYGKH"
     gene            251..1132
                     /locus_tag="VNR65_00160"
     CDS             251..1132
                     /locus_tag="VNR65_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HWJ17115.1"
                     /translation="MQQDFRSSTSTASIDESVADPFLPELYRVVSATTEIPDVVTMDI
                     VPVSGERPPFAAGQFNMLYVFGVGEIAISMSGDPADMSRHVHTIRDVGMVSGALTRLK
                     EGDVLGVRGPFGTGWPVHQAEGLDVVMVSSGCGLAPLRPAIYHILNNRERYGRVSILL
                     GFHSPNDLLYRHEFEQWRQRLDIDLLVTVNHADRSWRGNVGGVQVSNFIKRANFDPKE
                     TLAMVCGPEGKMRYMASSLRDAGVPEERIYFSMERNMKCAMGICGRCQFGPEFTCKDG
                     PVLRFDRISRILAVREM"
     gene            1134..1910
                     /locus_tag="VNR65_00165"
     CDS             1134..1910
                     /locus_tag="VNR65_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17116.1"
                     /translation="MATRNRPRLAVWKFASCDGCQLSLLDLEDDILTLAAEIDIANFP
                     EASSAISDGPYDLSLVEGSITTSHDAERIHKVREQSKFLVTIGACATSGGIQALRNFA
                     DVNSFISAVYASPQYINTLSTSTPIKSHVRVDYELQGCPINKLQLAEVISAYLAGRRP
                     SIPAYSVCIDCKLHGTPCVMVQGIPCLGPVTHSGCGAICPTFRRGCYGCFGPMETPNT
                     AALAKEWQALGATERDLQRVFRTFNAAAEPFRKESEAHGD"
     gene            1900..>2877
                     /locus_tag="VNR65_00170"
     CDS             1900..>2877
                     /locus_tag="VNR65_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HWJ17117.1"
                     /translation="MGTRSILVDYLARVEGEGALDLQVSDGRVTSAQLKIFEPPRFFE
                     AFLRGRGYEEVADIVARICGICPIAYQMGAVHAIESVFGAKVDGQLRALRRLIYCGEW
                     IESHGLHVVMLHAPDFLGFPDGIAMARDHGAMVKNGLAIKKAGNEIMQLLGGREIHPV
                     SVKAGGFHRVPTRAELNALAEKLKRARDLAVGLARWVALFPFPDFEQDYEFVALRHPD
                     EYPFNEGRLVSSRGLDIDIADYDNEFEERHVAHSTALHSVLKPRGAYLVGPLARYALN
                     FDLLPASVKALARDTGLGPVCKNPFRSIVVRAIEIVYAFEEALRIISDYE"
BASE COUNT          528 a          885 c          875 g          589 t
ORIGIN      
        1 gggaagatcg ctcttgagat cgtagcgccc ggccgcgagc cgatcgtctt cgagacgttg
       61 caagacgggg atattgttgg cgcgtcgtgg ctcgtcccgc cttatcgctg gatgttcgac
      121 gcacgcgccg ttggattgac ccgcgccatc ggcatggacg ctgcgtgtct gcgcgggaaa
      181 tgcgaggcgg atcatcacct tggctatgag atgatgaaac gctttttacc tatcctagta
      241 ggacggcttc atgcaacaag acttcagatc ctcgacgtct acggcaagca ttgacgagtc
      301 ggtggcggac ccgttcctgc cggaactgta tcgcgttgtc agcgcgacaa ccgagatccc
      361 ggatgtcgta acgatggaca tcgtgccggt ttccggggag cggccgcctt tcgctgccgg
      421 gcagttcaat atgctttacg tcttcggcgt gggcgaaatc gccatcagca tgagcggcga
      481 tcccgctgac atgagccgcc acgtgcatac catccgcgac gtcggcatgg taagcggcgc
      541 actgacaagg ctcaaagagg gcgacgtcct cggtgtgcgc gggccgttcg gaaccggctg
      601 gccggttcac caagctgagg ggctcgatgt cgtcatggtg tcgagcggct gcgggctcgc
      661 acctttgcgg cccgccatct accatatcct gaacaatcgc gagcgctatg ggcgcgtttc
      721 gatcctcctc ggcttccaca gcccgaacga tttgctctat cgccatgagt ttgagcagtg
      781 gcggcagagg ctcgacattg atctcctcgt gaccgtcaac cacgcagatc gcagctggcg
      841 cggcaatgtc ggcggcgtgc aggtgtcgaa cttcataaag cgcgcgaatt tcgatcccaa
      901 ggagacgctt gccatggttt gcggccccga gggcaagatg cggtacatgg ccagctccct
      961 tcgcgatgcg ggcgtccccg aagagcggat ctacttctcc atggaacgca acatgaaatg
     1021 cgccatgggg atttgcggcc gctgccagtt cggtcccgaa tttacctgca aagatggccc
     1081 ggtgctgcgg ttcgaccgca tctcgcgcat cctcgccgtc agggagatgt gacatggcta
     1141 cgcgaaaccg tcccaggctc gcggtctgga agttcgcatc ctgcgacgga tgtcagcttt
     1201 ccctgctcga cctggaggac gatattttga cgctcgcggc ggaaattgat atcgcgaatt
     1261 tccccgaagc gtcgagcgcc atttccgacg ggccttacga tctatctctg gtcgaaggat
     1321 cgattacgac ctcgcatgac gccgagcgca ttcacaaggt gcgcgagcaa tcgaaatttc
     1381 tcgtgaccat cggcgcttgt gcgacttcgg gcggcatcca ggcgctgcgg aatttcgccg
     1441 acgtgaacag cttcatctcc gccgtctatg cctccccgca atatatcaat acgctttcga
     1501 catcgacgcc gatcaagtcg catgtgcggg tggattacga actccagggc tgccccatca
     1561 acaagttgca actggctgag gttatctccg cctaccttgc gggccggaga ccgtcgatac
     1621 cggcctacag cgtttgcatc gactgcaagc tgcacgggac cccttgcgtg atggttcagg
     1681 gcattccctg ccttggcccc gtgacgcatt ccggctgcgg cgcgatttgt cccacgttcc
     1741 ggcgcggctg ctatggctgc ttcggcccga tggaaacgcc gaacactgcc gcgctggcga
     1801 aggagtggca ggcgctgggc gcgactgagc gcgatcttca acgcgtcttc cgcaccttca
     1861 acgccgccgc ggaaccgttc cggaaagaga gcgaagctca tggggactag gtcaattttg
     1921 gtcgattatc tcgcccgcgt cgaaggcgag ggcgcactcg acctgcaagt cagcgacggg
     1981 cgcgtgactt ccgcacaact caagatattc gagccgccgc gtttcttcga agcgttcctg
     2041 cgcggacgcg gctacgaaga agtcgccgat atcgtcgccc gcatttgcgg catctgcccg
     2101 atcgcctatc aaatgggcgc ggtccatgcg atcgagagcg ttttcggcgc gaaggtcgac
     2161 ggacagcttc gggcgctgcg ccgcctcatc tactgcgggg aatggatcga aagccacggg
     2221 cttcacgtgg tgatgctcca tgctccagac tttctcggct tcccggatgg catagccatg
     2281 gcgcgcgatc acggcgcgat ggtcaagaac ggccttgcta tcaagaaggc cggcaacgaa
     2341 atcatgcagc tccttggcgg ccgggaaatt caccccgtga gcgtcaaggc gggcggtttc
     2401 catcgcgtgc caactcgggc ggagcttaac gccctggcgg aaaagctgaa acgcgcccgc
     2461 gacttggccg tggggctcgc gcgatgggtg gcactgtttc ccttcccgga tttcgagcag
     2521 gactacgagt ttgtggctct acgtcatccg gacgaatacc cgttcaacga aggccgcctc
     2581 gtttccagcc gcggcctcga catcgacatt gccgattacg acaacgagtt cgaggagcgg
     2641 catgtcgccc actccacggc tttgcattcg gtcttaaagc cccgcggcgc ttatctcgtt
     2701 gggccgctcg cccgttacgc cctcaacttc gacctcctgc ctgcctcggt taaggcgctg
     2761 gcgcgcgaca ccggactggg gccggtttgc aagaacccgt tccgcagcat cgtcgtgcgg
     2821 gccattgaga tcgtgtacgc cttcgaagaa gcactgagga tcatttcgga ctacgaa
//
LOCUS       DATMGX010000010         3502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_28818, whole genome shotgun sequence.
ACCESSION   DATMGX010000010 DATMGX010000000
VERSION     DATMGX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28818"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VNR65_00175"
     CDS             <1..201
                     /locus_tag="VNR65_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17118.1"
                     /translation="FERQMGDVNRQMAEHKTWVLTPILGIAMQHLQSFQDFPVRQVGL
                     SADVGKTVEAVQSQILKIQKQN"
     gene            201..1706
                     /locus_tag="VNR65_00180"
     CDS             201..1706
                     /locus_tag="VNR65_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWJ17119.1"
                     /translation="MKFGKGTPSMNTIKTLLAAAIAFCMFVPAHAEEKRPNIIFIMAD
                     DLGNADLGYRGGQIQSPNIDKLAQEGVRLESFYGQQVCTPSRAALMTGRYPMRYGLQT
                     LVIFPGHSYGLPTDERTLPQALKEAGYKTLMTGKWHLGHADRKFWPQNRGFDHFYGNV
                     MGEVNYFTRERGGVIDWQRNGAFLKEDGYYTTLIGDEAIKLIDQQDGKTPFFLYFASL
                     APHAPYQAPDDYKDRYKSVEDMPRRTYAAMITALDDQIGRIVAELDKKGLRDNTIILF
                     ASDNGGATSGLFASGSKSKEERDTEEGGIEQGAKAPASNAPLRGGKGGIYEGGVRVPA
                     FVNWPAKLKPRVVDAPLHMVDVMPTLLALAGAKGSPDHPFDGKDMWKTLAEGASSPNE
                     DVLIDVEAFRGAVRKGDWKLVKVALLPGKTELFNLASDPGETTNVAEQNPEVVRDLEA
                     RLLAYAKQQKPSEWIKAQPAFVGEQGKTVFDPDFDIDDSGLPHEKAALPGG"
     gene            1769..>3502
                     /locus_tag="VNR65_00185"
     CDS             1769..>3502
                     /locus_tag="VNR65_00185"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWJ17120.1"
                     /translation="MGWMSRHVVLRALLGLVGQSFLWFVLAAAVLPGKAAAGPDDGSV
                     LPFAPAPSASVAEPRLQDSIHHRREEKSHLPKDAPNILIVLLDDVGFGLPDTYGGPVH
                     TPTLSRIANEGISYNTFHTTAICSPTRAALLTGRNHQRVGSGTIAERAVDWDGYTGVI
                     PRTSATLAKVLGEYGYKTAAFGKWHNTPATETTAMGPFTRWPTGEGVGFDYFYGFLAG
                     ETSQWEPRLYENLNPIEPPHDPKYHLSEDLADKAITWMKRHRAFSPDKPFFMYWAPGA
                     AHGPHHIFKEWADKYKGRFDDGWDDLRERVFKRQKELGWIPSDAKLTPRAESMVGWDS
                     IPEAERPFQRRLMEVFAGFTEHVDAQVGKLIDGLDELGAKENTLVFYVWGDNGSSAEG
                     QKGTISELLAQNGIPSTLDQHLKALDNLGGLDALGGPKVDNMYHAGWAWAGDTPFQYT
                     KLIASHFGGTRNPLAISWPARIKPDKTPRSQFHHVNDIVPTIYEILDIKPPKVVDGFE
                     QDPIDGVSMAYTFANAKAETHKHTQYFDNNGSRGIYHDGWYACTFGPLVPWDAAYNMK
                     LGEWDSSKDIWE"
BASE COUNT          748 a         1046 c         1082 g          626 t
ORIGIN      
        1 ttcgagcggc agatgggcga cgtgaaccgg caaatggccg aacacaaaac ctgggtgctc
       61 acaccgatcc tcggcatcgc catgcagcac ttgcagtcgt tccaggactt cccggttcgc
      121 caggttggat tgagcgccga tgtcggcaag acggttgaag ccgtccagtc gcagatcttg
      181 aaaattcaga agcaaaactg atgaaattcg gcaagggaac gccttcgatg aacacgataa
      241 aaacccttct cgccgcagca atcgcattct gcatgttcgt ccccgcccat gcggaggaga
      301 agcggccgaa catcatcttc atcatggccg acgatttggg taacgccgat ctcggctatc
      361 gaggcggcca aattcaatcg ccgaacatcg acaagcttgc acaagagggt gtccggctcg
      421 agtctttcta tggtcagcag gtgtgcacgc cgtcgcgggc ggcgctgatg actggccgct
      481 atcccatgcg ctatggattg cagacgctag ttatctttcc gggtcacagc tacggtctgc
      541 ctacggatga gcgcacgctg ccgcaagcgc tgaaggaggc cggttacaaa acgctgatga
      601 ccggcaaatg gcatctcggc catgcggaca ggaagttctg gccgcaaaat cgaggcttcg
      661 atcatttcta cggcaacgtc atgggcgagg tgaattattt cacccgcgag cgtggcggcg
      721 tgatcgactg gcagcgcaac ggcgccttcc tgaaggagga cggctactac acgacgctga
      781 ttggagacga ggcgatcaag ctgatcgacc agcaggacgg caagactccc ttcttcctct
      841 acttcgcctc gcttgcgccg catgccccct atcaggcgcc ggatgactac aaggaccggt
      901 acaaatcggt tgaggatatg ccgcggcgca cctatgcggc catgatcacg gccctcgacg
      961 accagatcgg acgcatcgtc gccgaactcg acaagaaagg tctgcgcgac aacacgatta
     1021 ttctcttcgc cagtgacaat ggcggagcca cgagcggcct tttcgccagc ggctcgaaga
     1081 gcaaggagga gcgcgataca gaagaaggcg gcatcgagca gggagcaaag gctccggcct
     1141 ccaacgcgcc tctcaggggc ggcaagggcg gcatttacga gggcggcgtg cgcgtgccag
     1201 cctttgtaaa ctggccggcc aagcttaaac cgcgcgtggt cgacgcgccg ctgcacatgg
     1261 tcgacgttat gccgacgctc ctcgcgctcg ccggcgcgaa gggcagtccc gatcatccct
     1321 tcgacggcaa ggacatgtgg aagaccctcg ctgaaggtgc gtccagcccc aacgaggacg
     1381 tgctgatcga tgtggaggcc ttccgcggcg ccgtccgcaa gggcgattgg aagctcgtga
     1441 aggtggcgct cctgcccggc aagacagagc tcttcaatct cgcgagcgat cccggcgaaa
     1501 caacaaacgt cgccgaacag aaccccgagg ttgtgcgcga tctcgaagca cgccttctcg
     1561 cttacgcgaa gcagcagaag ccgagcgagt ggatcaaggc ccaaccggcc tttgtgggcg
     1621 agcagggaaa gacggtgttc gatcccgact tcgatatcga cgacagcggc ctcccgcatg
     1681 aaaaggcggc tttgccggga ggataagcgc ggctttgaaa ttggttggtc cgctcgcgag
     1741 caattagggt ttggagcagg gggttggtat gggttggatg tcacggcacg tggtacttcg
     1801 cgctctgctt ggacttgttg ggcagagctt cctctggttc gttttggcgg cggcggtatt
     1861 gccaggcaag gcggccgccg gtccagacga tggatccgtc ctgcccttcg caccggcgcc
     1921 ttcggcgagc gtggccgaac cccgattgca ggattccatc caccatcggc gcgaggagaa
     1981 aagccatctg ccaaaggacg cgcccaatat cctcatcgtg cttctggatg acgttggctt
     2041 tgggttgccc gacacctatg gcggcccggt tcatacgccg acgctgtcgc gtatcgcgaa
     2101 tgaggggatc agttacaaca cattccacac gaccgcgatt tgctcaccga cgcgcgctgc
     2161 cctgctgacg ggacgcaatc atcagcgcgt cggctcggga accatcgccg agcgcgccgt
     2221 ggattgggac ggttatacgg gcgtgatccc gagaacatcg gcgacgctcg ccaaggtgct
     2281 cggcgaatat gggtacaaga ccgccgcctt cggcaaatgg cacaacacgc cagcgaccga
     2341 gaccacggcg atggggccat ttacgcggtg gccgaccggt gagggcgtcg gctttgacta
     2401 tttctatggc ttcctggcgg gcgagacatc gcaatgggag cctaggctct atgagaatct
     2461 caatcccatc gagccgccgc acgacccgaa ataccatctg agcgaagacc tcgccgacaa
     2521 ggcgatcacc tggatgaaac gccatcgcgc attctcgccc gataagccgt tcttcatgta
     2581 ttgggcgccg ggcgccgcgc atgggccgca ccacattttc aaggagtggg ccgacaaata
     2641 caaagggcgc ttcgatgacg gctgggatga cctgcgcgag cgcgtcttca agcgccagaa
     2701 ggagctgggc tggattccgt cggatgccaa actgacgccg cgcgcggaaa gcatggtcgg
     2761 atgggacagc atcccggagg cggagcgacc gttccagcgg cggctcatgg aagttttcgc
     2821 tggattcacc gagcatgtcg acgctcaggt cggcaagctg atcgacggtc tcgacgaact
     2881 cggcgcgaaa gagaacacct tggtcttcta cgtctggggc gacaacgggt cgagcgccga
     2941 aggccagaag gggacgatta gcgaactttt ggcccagaac ggcattccca gcactctcga
     3001 tcagcatctc aaggcgctcg ataacctcgg gggacttgac gcgctcggcg gcccgaaagt
     3061 cgacaacatg tatcatgccg gctgggcctg ggccggcgac acgccgttcc aatacacgaa
     3121 gctgatcgcc tcgcatttcg gcggtacgcg caacccgctc gccatttcct ggcccgcgcg
     3181 catcaagccg gacaagacgc cgaggtcgca gttccaccat gtcaacgaca tcgtgccgac
     3241 gatctacgag atccttgata tcaagccgcc gaaagtggtc gatggcttcg agcaggatcc
     3301 catcgacggg gtcagcatgg cctacacctt cgcgaacgcc aaagcggaga cgcacaagca
     3361 cacgcaatat ttcgacaaca acggcagccg tggcatttat cacgacggct ggtatgcctg
     3421 cacatttggg ccgctcgtcc cttgggacgc agcctacaat atgaagctgg gagagtggga
     3481 ttcgtccaag gacatttggg ag
//
LOCUS       DATMGX010000011         2914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_28780, whole genome shotgun sequence.
ACCESSION   DATMGX010000011 DATMGX010000000
VERSION     DATMGX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28780"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VNR65_00190"
     CDS             <1..488
                     /locus_tag="VNR65_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ17121.1"
                     /translation="FMSIESAPGQGCTVSIYLPRTTRGSAARGVLPGSSPLGRGQLIL
                     VVEDDDDVREVTLKRLEMLGYTVTEARTGPEALERLTLQEGVRLVLSDIVMPGGMTGY
                     DVARWVASNKPDVKVIMCSGYNEGDQAIGAQGAIDGVAMLGKPYTRDQLARALSEAFA
                     H"
     gene            750..1664
                     /locus_tag="VNR65_00195"
     CDS             750..1664
                     /locus_tag="VNR65_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ17122.1"
                     /translation="MSFGNGPSGGPKILAVDDEAFNLDLMEHHLGRAGHHVVRAEDGV
                     IALQKLEEEPRIDLIVLDRMMPNLGGMEVLRRIKADARFQDIPVVMQTAAAAADQVSE
                     GIKAGAYYYLTKPYKGATLVGIVRAALDDARTKARLKEKVGACARGLGLMEEARFRFQ
                     TMEDAAGLASYVSNCFPDPQSVVIGLHELLLNAVEHGNLGITYSEKTNLVRNATWNEE
                     VERRLALPEYCKNFATLTFQATEAALTVHIKDAGTGFDWKHYLDFSADRATDPHGRGI
                     ATSRALSFHSLEYLGVGNEVRCTVKLNP"
     gene            2131..>2914
                     /locus_tag="VNR65_00200"
     CDS             2131..>2914
                     /locus_tag="VNR65_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWJ17123.1"
                     /translation="MATRREFVQALPAVGAAFAIGGHLVLEDSPAQAQAATAPLEGHF
                     HPKGKAPSKHTLELLKQARGGLPFDDTRDFEEQKKGLIAPMPDLKIMADAGHVAWDME
                     RFQFLDKQDDFDSIHPSLLRQSKLNNNYGLYEVIPGIYQVRGFDLSDITFIRGKTGWI
                     VFDPLVSREPVRAAWKLFQEHVGQGLPVSAVIYSHTHGDHWGGVRGIVDEADVRSGKV
                     PVIAPVDFMEFTISENVYAGNAMNRRLFYQYGLLLPASPHGYV"
BASE COUNT          635 a          796 c          869 g          614 t
ORIGIN      
        1 ggtttatgtc gattgaaagc gcgccggggc aagggtgcac tgtcagcatc tacctgcctc
       61 gcacaaccag gggatcggcg gcgcgtggcg ttctgccggg tagtagcccc ctcggccgcg
      121 gtcaactcat tctggtggtc gaagatgatg atgatgtccg cgaagtgacg cttaagcggc
      181 ttgaaatgct tggctatact gtcacagaag caagaacggg tccagaagcc cttgagcggc
      241 tcacattgca agagggggtg cggcttgtac tgagcgatat cgtcatgcca ggcggcatga
      301 cgggctacga cgtggcgcgc tgggtggcgt cgaacaagcc agacgtcaaa gtcatcatgt
      361 gctctggtta taacgaggga gatcaggcca ttggcgctca aggggcgatc gatggcgtcg
      421 ctatgcttgg caagccctac acccgggacc aactcgcacg cgcgttaagc gaggcgttcg
      481 cacactagag cccgcatcaa attggacgct ctaacccagc gtgcgcaaaa tcggagccgg
      541 ttgcgacgca ctttttcttg gctgtgatta tccgccaatc tgaaacctgt aacttcgtga
      601 aattggcgct ttcacagtaa ggcaaatcaa accagaaccg tggattatgt gcaggccaag
      661 ctccttcccg gaagcaactc aagcgaggtt tacactggcc ggggctggag cggcattaga
      721 gcagaactcg aaacaagaac agccagacca tgagctttgg gaacggcccg agcggcggac
      781 cgaaaattct tgccgttgat gacgaggcgt tcaacctcga cctcatggag catcatttgg
      841 gccgcgctgg ccatcacgtg gtgcgcgctg aagacggcgt tatcgccttg cagaagctgg
      901 aggaagagcc ccgcattgat ttgatcgtcc tcgataggat gatgccgaac cttggcggca
      961 tggaagtctt gcgccgcatt aaggccgacg cccgctttca ggacatcccg gtggtcatgc
     1021 aaacggcggc ggccgcggcg gaccaagtct cggagggaat caaggcaggc gcttattact
     1081 atctgaccaa gccctacaag ggcgcgaccc ttgttggcat cgtaagagcc gctctggacg
     1141 atgccagaac caaggcgagg ttgaaagaaa aggtcggcgc ttgcgcccgg gggcttggcc
     1201 tcatggagga ggcccgcttc cgttttcaaa ccatggagga cgctgccggc cttgcctcct
     1261 atgtctccaa ttgctttcca gacccccaaa gtgtggtcat tggattgcat gagttgctgc
     1321 tgaacgcggt tgagcatggc aatctcggca tcacttatag cgaaaagacc aacctcgttc
     1381 gaaacgcaac ctggaacgag gaggtcgaac gcaggctcgc cctaccggaa tattgcaaga
     1441 atttcgcgac cctcaccttc caggcaacag aagcggcgct cactgttcac atcaaggatg
     1501 cgggcacagg attcgactgg aaacactatt tggacttctc ggccgatcga gcgacggatc
     1561 cgcatggacg gggaattgcc acctcgcgcg cgctatcctt tcacagcctc gaatatcttg
     1621 gggtgggaaa tgaagtcaga tgcaccgtga agttgaaccc atagcctcac aaagttgtga
     1681 cccaaccttg tttttaattt atgtcaattt cgactacctt gcagaaactt gctcttaaac
     1741 tgctcagaca atcagagatg aaagcatgct tcggtcagct gccgtggcaa ttcccttgac
     1801 tgtcgcgatt ttaggcgtgg gctctgctga tggttctaag taatgcacga gttatgcagc
     1861 aaggtttatt actgaaaatc gcaatagttt cggctgctgc atttttcatt tcgatctctg
     1921 gggctgaagc catcaccctt tccgcaagcg acgagatgcc ggctgtccgc tccgaagccg
     1981 tcaaagtcca atgtgtcagc ggaaggcggt gcgttggcgg ctggggctgg cgtggtcccc
     2041 gccgtttttg ccgcagatgg gttgtgtgcc gatagggatg aataaataag ccgtgcacgt
     2101 gttgacgggg cactttgatt ggagagggcg atggcaacca gacgagagtt cgtgcaggca
     2161 cttccagcgg taggcgcagc attcgcaatc ggcgggcact tggtgcttga ggatagtccc
     2221 gcgcaggctc aagcagcgac cgcgccattg gaggggcact tccatcccaa gggcaaagcg
     2281 ccctcaaaac acacgttgga gttgctcaaa caggcgcgcg gcggcttgcc cttcgacgac
     2341 acgcgggatt ttgaggagca gaagaagggg ctgatcgctc ccatgccgga tctcaagatc
     2401 atggcggacg ccggccacgt cgcctgggac atggagcggt ttcagtttct ggacaagcaa
     2461 gacgacttcg acagcattca tccctcgttg ttgcgtcagt cgaagttgaa caacaattac
     2521 ggactctacg aagtgatccc gggcatctac caggttcgcg gcttcgatct gtccgacatc
     2581 accttcatcc gcggcaagac gggctggatc gtgttcgatc ccctggtgag cagggaaccc
     2641 gtccgcgcgg cgtggaagct gtttcaagag catgttggcc agggccttcc cgtctcggcc
     2701 gtgatctatt cgcacaccca tggcgatcat tggggcggcg tgcgcggtat cgtcgacgaa
     2761 gccgatgtga ggtccggaaa ggttccggtc atcgcgcccg tggacttcat ggagttcacg
     2821 atttccgaaa acgtctatgc cggcaacgcc atgaaccggc gcctgtttta tcaatacggc
     2881 ctcttgttgc cggcaagccc ccacggctac gtcg
//
LOCUS       DATMGX010000012         2096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_28413, whole genome shotgun sequence.
ACCESSION   DATMGX010000012 DATMGX010000000
VERSION     DATMGX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28413"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VNR65_00205"
     CDS             <1..229
                     /locus_tag="VNR65_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17124.1"
                     /translation="GIFSSLPKYLMTLRKCRCLSTAPVLHKRAYGLSIVITSATCSGD
                     TARQVSARDIATVISRAEGIAAWHFDQPAEI"
     gene            248..>2096
                     /locus_tag="VNR65_00210"
     CDS             248..>2096
                     /locus_tag="VNR65_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HWJ17125.1"
                     /translation="MNEMTIDKTFTETHEMTGKCPYGGDRVGGAFGTPPTLENWYPDR
                     LRVELLHQNGLAADPLGADFDYAAEFAKLDFEAVKRDIKQFLTTSVAWWPSDYNNYGP
                     QMIRMAWHAAGTYRIADGRGGAGTAMQRFAPISSWWDNGNTDKSRRLLQPIKHKYGNG
                     LSWGDLMILTGNCALEIMGFPTYGFGGGRLDAWEADNATYWGPEIWNPKDAGSFDSMV
                     MRDKRWRGKSGDADYDLENPLAASHQALIYVNPEGPYASGDPMGSARDIRITFTRMAM
                     NDEETVALIAGGHAFGKSHGMVSATEVGPPPEIAPMEAMGLGWHNPKGPGFAQYAMTN
                     GIEGSWTPNPTQWDNSYLENLFKFEWEQIKSPAGALQWTPTDPNAPKTPDAHVEGQMN
                     NLMMMTSDIALKLDPEYRKVCEKFLTDFEAFTQAFSKAWYKLTHRDMGPKHRYLGPEV
                     TIEDGLLWQDPLPGRDYELVGEAEVAGLKQAIMATGLSVSDLAFTAFSAAATYRDSDK
                     RGGANGGRLALAPQKDWPVNRRAAPVIEKLRGVMSDFNGKQGAGTKISLADLIVLGGC
                     VAVEKAARDAGVDDASVPFTPGRVDTTQDLTDVEMFEWLKPIVDGFRNYV"
BASE COUNT          454 a          604 c          650 g          388 t
ORIGIN      
        1 cgggatcttc agctcattgc ccaaatactt gatgacattg agaaaatgcc gctgcctgag
       61 caccgccccg gttctccata aacgggccta cggattgtcg attgtcatca cctcggcaac
      121 ttgcagcggc gataccgcgc gccaagtgtc tgcaagagac atcgcaacgg tgatttcgcg
      181 tgctgagggt atagcagctt ggcactttga tcagccggcg gagatttaac ccgctaagga
      241 tgtttcgatg aacgagatga cgatagacaa gacctttacc gaaacacacg agatgaccgg
      301 caagtgtcca tacgggggtg acagagttgg cggtgcattc ggtacccctc caaccctgga
      361 gaactggtat cccgatcgcc tgcgggtcga acttctccat cagaacggcc tggcggcaga
      421 tccgcttggc gcggatttcg actatgctgc tgagttcgcg aagctcgatt tcgaagcggt
      481 caagcgtgat atcaagcagt ttctgaccac atcggttgcc tggtggccgt cggactataa
      541 taactacggt ccgcagatga tccgtatggc gtggcacgca gcgggcacct atcgaatcgc
      601 tgacgggcga ggcggtgccg ggacggcgat gcaacgcttc gccccgatca gtagctggtg
      661 ggacaatggc aataccgata agtcgcggcg cctcctgcaa ccgatcaagc acaagtacgg
      721 caatggtctt tcctggggcg accttatgat cctgacgggc aattgcgccc tcgagatcat
      781 gggcttccca acctatggtt tcggcggcgg acggctcgac gcgtgggagg ccgacaacgc
      841 cacctattgg ggcccggaaa tctggaaccc gaaggacgcc ggcagcttcg acagcatggt
      901 catgcgcgac aagcggtggc gcggcaagag cggcgatgcc gattacgacc tcgaaaatcc
      961 gcttgccgct tcgcatcagg cgctcatcta cgtcaatccc gaaggtccct atgcaagcgg
     1021 cgatccaatg gggtctgcac gcgatattcg gatcaccttc acgcgcatgg cgatgaatga
     1081 tgaagaaacc gtggccctga tcgcgggcgg ccatgccttt ggcaagagcc atggcatggt
     1141 gtctgcgaca gaggtagggc cgccgcccga aattgcgccg atggaagcca tgggccttgg
     1201 ctggcacaat cccaagggcc ccggtttcgc gcaatatgcg atgaccaacg gcattgaggg
     1261 cagctggacg ccgaacccga cgcagtggga caacagctat ctcgaaaacc tgttcaagtt
     1321 cgaatgggag cagatcaaga gcccggccgg tgcgctgcag tggacgccga ccgacccgaa
     1381 tgcgcccaag acgcccgacg cgcacgtcga gggtcagatg aataatctta tgatgatgac
     1441 cagcgatatc gcgctcaagc tcgacccgga gtatcggaaa gtctgcgaaa aattcctgac
     1501 ggatttcgag gcattcacac aggcgttttc caaggcgtgg tacaaactca cccaccgcga
     1561 catgggaccc aaacatcgct acttgggccc agaagtgacg atcgaggacg gcctcctttg
     1621 gcaggacccg cttcccgggc gagactatga gttggtcggc gaggcggaag ttgccgggct
     1681 aaaacaggca atcatggcga ctggcctttc ggtttccgat ctggccttca ccgccttctc
     1741 cgcggcggcg acgtatcgcg acagcgacaa gcgtggcggc gcgaatggtg gtcgccttgc
     1801 gctcgcaccc cagaaggact ggccggtcaa tcgccgggcc gcgccggtga tcgaaaagtt
     1861 gcgcggagtc atgtccgact tcaacgggaa acaaggcgcc ggcacgaaga tctcgctagc
     1921 cgacctgatc gtgttgggtg gctgcgtggc ggttgagaag gcggcaagag atgccggggt
     1981 cgacgacgcc tcggttccgt tcacgccggg ccgtgtggat acgacgcagg acctcaccga
     2041 cgtcgaaatg ttcgagtggt tgaagccgat cgtagacggc ttccgcaact acgtcg
//
LOCUS       DATMGX010000013         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_373726, whole genome shotgun sequence.
ACCESSION   DATMGX010000013 DATMGX010000000
VERSION     DATMGX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373726"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VNR65_00215"
     CDS             <1..973
                     /locus_tag="VNR65_00215"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HWJ17126.1"
                     /translation="NDIAFTPEARTPDPQRLLKAYRQSAATLNLLRAFAQGGYADLER
                     VHQWTLGFVKDSPAGNRYEELAGRISETLNFMRACGVTSENSERLRTTDFYTSHEALL
                     LGYEEAFVRIDSTSGKHYATSGHFLWVGDRTRQPDHAHIEFLRGIRNPIGVKCGPSIK
                     PQELIAIIDVLNPANDPGRLTLICRFGADKVGAHLPDLIRAVKREGKTVIWACDPMHG
                     NTIKAASGFKTRPFNAILREVEAFFDVCQAEGVYPGGLHVEMTGKNVTECTGGAQSIS
                     DADLSDRYHTYCDPRLNADQALELAFLVAERLRRDRVYVEPKLSALS"
     gene            1102..2331
                     /locus_tag="VNR65_00220"
     CDS             1102..2331
                     /locus_tag="VNR65_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWJ17127.1"
                     /translation="MDIAVPHSIPFDEIPLFARLPKHRRDALEQNIRICRFDAGERIF
                     EEGAPNPGRFYIVLEGEVSLCNRGQAFSTHTMADYEISVRRKNEVFGAISFLDGEPFS
                     SSAFAKTPVTLAVLCFSEAGSSPELRKLRAAVVAELRRQLTSDLRLSITDRLGSLQQE
                     AEFARYKNAVGRIVVTTLAILSFYTLLLSLLPQFMVALAVNFAISPFIIIIFASVMGP
                     VILYSGFPPSFFGLQTANWKSAVAFSVAASLAFIAVGALLKWILINTTQTFAGLSLFG
                     VADVKVDMHQVMTSPLYWVAVGLYLLLTPIQEFVARCCLQAPLYAFLNGTEFSRRILS
                     ILVSNLVFAAAHSHISFAFAVAAFIPGLFWGWIFARTNSLIAASASHFMIGGAGIFLF
                     GIEEVVDRLMHGMMSAM"
     gene            complement(2338..>2633)
                     /locus_tag="VNR65_00225"
     CDS             complement(2338..>2633)
                     /locus_tag="VNR65_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HWJ17128.1"
                     /translation="THVPTADARVLPHGTAFMTDAGMCGDYNSIIGMDKGEPLHRFTA
                     RIPSSRFTPALGPATVCGALIEIDDATGLAVSIEPVREGAPLGGGVRDLKREL"
BASE COUNT          458 a          814 c          807 g          554 t
ORIGIN      
        1 caacgacatc gcgttcacgc ccgaggcgcg cacgcccgat ccgcagaggc tgctcaaggc
       61 ttaccggcag tcggcggcga cgctcaatct ccttcgagcc ttcgcgcaag ggggctatgc
      121 ggacctcgag cgcgtccacc aatggacgct gggcttcgtg aaggacagcc ccgccgggaa
      181 ccgctatgag gaattggcgg gccgtatctc ggagacgctg aacttcatgc gcgcctgcgg
      241 cgtcacctcc gagaattcgg agcggctccg caccaccgat ttctacacga gccacgaggc
      301 gctgctgctc ggctacgagg aagccttcgt gcggattgat tcgaccagcg gcaaacacta
      361 cgcgacatcg ggccatttcc tctgggtcgg cgaccgcacg cgccagccgg atcacgcgca
      421 tatcgaattc ctgcgcggca tcaggaaccc gatcggcgtg aagtgcgggc cgagcatcaa
      481 gccgcaagag ttgatcgcta tcatcgacgt gctgaacccg gccaatgatc caggccgcct
      541 gacgctgatt tgccggttcg gcgcggataa ggtcggcgcc catcttcccg acctcatccg
      601 cgcggtgaag cgggaaggca agacggtgat ctgggcctgc gatcccatgc acggcaacac
      661 gattaaggcg gcgagcgggt tcaagacgcg gcccttcaac gcgatccttc gcgaggtcga
      721 ggccttcttc gacgtctgtc aggccgaggg tgtttatccc ggcggcctcc atgtcgagat
      781 gacgggcaag aacgtgaccg aatgcacggg cggcgcgcag tccatctcgg atgcggatct
      841 gtccgaccgg tatcacacct attgcgatcc gcgtctcaat gcggatcagg cgctggaact
      901 cgcgttcctc gtcgcggagc ggttgcgtcg cgatcgcgtc tatgtcgagc cgaagctttc
      961 ggcgctctct tgaacgagcc tcgcggggca ggcaatcata ttgccgcctg cccggaaagg
     1021 gccagaaagc aataacgaac ttttgtagtt cctgattgcc gagaggcgtt tctattgggg
     1081 cctcacaaaa agggttttcc gatggatata gccgtccccc acagcatccc gttcgatgag
     1141 atccccctgt tcgcccgcct gccgaagcac cggcgcgatg ctctggagca gaatatccgc
     1201 atctgccgct tcgatgcagg cgaacgtatc ttcgaggaag gcgcacctaa tcccggccgc
     1261 ttttacatcg tgctggaagg cgaagtctcg ttgtgcaaca gggggcaagc cttctcgacg
     1321 cacacaatgg ccgattatga gatctccgtg cgccgcaaga acgaggtctt cggcgccatc
     1381 tcgtttctgg acggcgagcc attttccagc agcgcgtttg ccaaaacgcc ggtgacgctt
     1441 gcggtgctct gtttttccga agccggttca tctcccgaat tgagaaagct ccgcgcggct
     1501 gtcgtcgccg aactcaggcg gcagctgaca tccgatctgc ggctctcgat caccgaccgc
     1561 ctgggcagcc tgcagcagga ggccgaattt gcccgctaca agaacgcggt cgggcgcatc
     1621 gttgtgacga ccctggcaat tctgtccttt tatacgctgc tcctgagcct cctgccgcag
     1681 ttcatggtcg cgctcgcggt caatttcgcg attagcccgt tcatcatcat catctttgca
     1741 tcggtcatgg gaccggtgat tctctatagc ggctttccgc catcgttctt tggcttgcag
     1801 acggccaatt ggaaaagcgc ggttgcattt tcggtggccg cgtcgctcgc cttcatagcg
     1861 gtgggcgcgc tcttgaagtg gatcctgatc aatacgacgc agacctttgc cgggctttcc
     1921 cttttcggcg tcgccgatgt aaaggtggac atgcatcagg tgatgacgtc gccgctctat
     1981 tgggtcgccg tcggccttta tcttttgctg accccgatcc aggaattcgt ggcgcgctgc
     2041 tgcttgcagg cgccgctcta cgcgtttctc aacggcaccg agttttcgcg caggatcttg
     2101 tccatcctgg tctcgaacct cgtcttcgca gcggcgcatt cgcacatcag ttttgcgttt
     2161 gcggtcgcgg ccttcattcc ggggctgttc tggggctgga tctttgcgcg aacgaactcg
     2221 ctcatcgcgg cttccgcctc tcacttcatg atcggtggcg cgggtatctt cctgttcggc
     2281 attgaggaag tcgtcgacag gttgatgcac gggatgatga gcgcgatgtg aggggggtca
     2341 cagctcgcgc tttaagtccc ggacgccgcc gccaagagga gccccctcgc ggacaggctc
     2401 gatggaaacc gcgagccccg tggcatcgtc aatttcgatc agcgcgccgc agacggtggc
     2461 cgggccgagc gctggcgtga aacggctgga cggaatgcgc gccgtgaacc tgtgcaaggg
     2521 ctcgcctttg tccatgccga tgatggagtt gtagtcgccg cacatcccgg cgtcggtcat
     2581 gaatgccgtg ccgtgtggca ggacccgagc gtcggctgtc gggacatgcg tgt
//
LOCUS       DATMGX010000014         3552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_27140, whole genome shotgun sequence.
ACCESSION   DATMGX010000014 DATMGX010000000
VERSION     DATMGX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27140"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VNR65_00230"
     CDS             <1..283
                     /locus_tag="VNR65_00230"
                     /inference="COORDINATES: protein motif:HMM:NF025044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWJ17129.1"
                     /translation="VLGGTLSPLDGRGPDDLSIAKLIERAHASEVAEIILALNATVEG
                     QSTAHYIAAELEGLPVKITRLARGVPIGGELDYLDEGTLAAAIKLRQPV"
     gene            371..2872
                     /locus_tag="VNR65_00235"
     CDS             371..2872
                     /locus_tag="VNR65_00235"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HWJ17130.1"
                     /translation="MTEQSPSTEERLVEAKVRAAKRAMLFETLWPRLWLPVAVCGVFV
                     LLSAFEVWQWLPPRFHLSLLGLFGGALVLSLVPLVLWKRPTREKSLARIEQASALEHR
                     PLTAYNDTISHEAASPETAALWQAHRDRAAQSLAKLKSGPARPRVDRYDPFALRALLV
                     LALVAVTVWASTDLPGRVEAAFKVPEIRGAAPDFRIDAWISPPAYTRKEPFVLANASG
                     ANEAISVPQGSLLTVKINGSGAKDYKVSLNSPEHSQTLEPVAQAGDTYAEFTRTLDQS
                     ATLVIGGLLETVKSWPLTVLPDHAPTIAFAGPIEISNRAVMLFKYKVDDDYGVSSAEA
                     HIDRIVEAPEGRASSSEPAQQIGKPPVFPLSLPRAPVRAADGKTYKDLTAHPWAGLPV
                     VVTLSAKDEAGHTGYSAARGLILPERKFTKPLAKAVIGQRRSLVENPSDTAPVASNLN
                     ALGITAEDEGTASTIYLGLRSAYWRLRHSPTMDEVESVVDQLWDVAVRIEDGNLSSAE
                     RDLRASQERLKDAIERGASQEEIQKLMSELRQALNTYMQALRQQQGKNDKAARSPNSQ
                     TISPQDLQQLLDKIERLAKSGSADAAAQMLNELRDILESIQTAKGGSEEEDKESMQRL
                     DRMTDILRQQQQLLDDTFRAQQGNEGDQSDREQQAQRGQRGQGQRQQGERGQQGQGKS
                     SSDLRKRQDDLQRQLQELLGDMGANEKDPTMKKLQEAEGAMGDAGQALQQNDLSDAGD
                     QEGRAVENLRQGARSMAQQMMRSGGQRGGSQANRDPLGRKQGDRLDDGESVKVPDEIT
                     VQRARTILEELRKRLGQPSRPPIELDYLERLVKPY"
     gene            2948..3388
                     /locus_tag="VNR65_00240"
     CDS             2948..3388
                     /locus_tag="VNR65_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HWJ17131.1"
                     /translation="MQIDELVDNFSFLDDWEDRYRYLIELGRALDPLSDTERTPETKV
                     RGCASQVWLISHAKGQGPDAVLDFRGDSDAHIVRGLIAVLFALYSGRTAREILTTDEK
                     AVLAKLGLNDHLTPQRSNGLASMVARIKRDALAASGAETTAVVQ"
BASE COUNT          734 a         1071 c         1091 g          656 t
ORIGIN      
        1 cgtgctgggc ggcacgcttt cgccgctcga tgggcgcggg cctgatgatc tgtccatcgc
       61 gaagctgatc gagcgcgctc atgcctcgga agtcgccgaa attatcctcg cgttaaacgc
      121 gacggtggaa ggccagtcga ccgcacatta tatcgcggca gagcttgagg gcttaccggt
      181 caaaataaca cggctcgcgc gcggcgttcc catcggcggc gagctcgatt atctcgatga
      241 gggcacgctc gcggcggcga tcaagctccg ccagccggtg taaccacgcc cgctgaacac
      301 gcataggaag ccacattttg tatggtatcg tccttccatg atgtcgaata aagataagtc
      361 cgaaagcacc ttgacggagc aatccccttc cacagaagag cgcctggtcg aggcaaaggt
      421 acgggcggca aagcgcgcga tgcttttcga gacgctgtgg ccccggctgt ggcttccggt
      481 ggcggtttgc ggtgtcttcg ttcttctctc ggctttcgaa gtatggcaat ggctgccgcc
      541 gcgcttccat ttgagcttgc tcggtctctt cggtggagcg cttgtgcttt cccttgtgcc
      601 gctggtttta tggaaacggc cgacgcgcga gaaatccctc gcgcgcatcg agcaggcctc
      661 cgcgctggag catcggccgc tcacggcgta caacgacacg atctcccacg aagcggcttc
      721 tccggaaacc gccgccttat ggcaggcgca ccgcgaccgc gccgcacagt cgctcgccaa
      781 actgaagtct ggtcccgccc gtccgcgcgt cgaccgctac gatcccttcg cgttgcgcgc
      841 gcttcttgtc ctggcgcttg tggcggtcac ggtctgggcc tccaccgatt tgccgggccg
      901 ggtcgaggcc gcattcaagg tgccggaaat ccggggcgcc gcgcccgact tccgcatcga
      961 cgcctggatc tctccgcctg cctacaccag gaaagagccc tttgtgcttg ccaatgcgtc
     1021 aggcgcaaac gaggccattt ccgttccgca agggtctttg ctgactgtta aaatcaacgg
     1081 atcgggcgcg aaagactaca aggtttctct gaactcgcct gagcattccc aaacgctgga
     1141 accggtggcc caggccggcg acacctatgc cgagttcacg cggacgctcg atcaaagcgc
     1201 tactctcgtc atcggggggc ttctggagac tgtaaaatca tggccgctca cggtgttgcc
     1261 cgatcatgcg cccacgatcg cattcgccgg gccgatcgag atatcgaatc gcgccgtcat
     1321 gcttttcaaa tacaaggtgg atgacgacta cggcgtttcg agcgccgagg cgcatatcga
     1381 tcgaatcgtc gaggcgccag agggccgcgc ttcctcttcc gagccagcgc agcagatcgg
     1441 taagcctccc gtctttccgc tttctcttcc gcgcgcgccc gtgagggccg ctgacggcaa
     1501 gacatacaag gatttgaccg ctcatccttg ggcaggtctt cccgtggtgg taaccctatc
     1561 cgccaaggat gaagccggac acacgggcta cagcgccgct cgcggcctta tcctgccgga
     1621 acggaagttc acgaagccgc ttgcgaaagc cgtcatcggc caaaggcgct ctctcgtcga
     1681 gaacccgagc gataccgcac ccgttgcaag caacctcaat gcgcttggca tcaccgccga
     1741 agacgaagga acagcttcga cgatttacct cggcctgcgg tctgcgtatt ggcggctgag
     1801 gcattcgccc acaatggacg aggttgaaag tgtcgtcgat cagctctggg acgttgccgt
     1861 tcgcatcgag gatgggaacc tgtccagcgc cgagagagat ttgcgggcct ctcaggaacg
     1921 cttgaaggat gccatcgaac ggggcgcgtc ccaggaagag attcagaagc tgatgtccga
     1981 gcttcgccag gcgctgaaca cctatatgca ggcgctcagg cagcagcagg gcaagaacga
     2041 caaggcggcg cggtcaccaa attcgcaaac gatctcgccc caggatttgc agcagcttct
     2101 cgataagatc gaacgtctgg caaaatcagg gtcggccgat gccgccgccc agatgttgaa
     2161 cgagctgcgc gacatcctgg aatccatcca gaccgcgaaa ggcggctcgg aagaagaaga
     2221 caaagagagc atgcagcgtc tcgaccgcat gaccgatatc ctgcggcagc agcagcagct
     2281 tttggacgac actttccggg cgcagcaggg aaacgaaggc gatcaaagcg acagggagca
     2341 gcaagcacag cgcggccaac gcggccaggg gcaaaggcag cagggggagc gtggacaaca
     2401 gggccaaggc aagagctctt cggacctgag aaagcgccag gacgatctcc agcgccagct
     2461 tcaggagctt cttggcgaca tgggtgcgaa cgaaaaagat ccgaccatga agaagctgca
     2521 agaggccgaa ggcgcgatgg gcgatgccgg ccaagcgctg cagcagaatg atctcagcga
     2581 cgctggcgat caggaaggcc gtgcggtgga gaatctgagg cagggagcgc gcagcatggc
     2641 ccagcagatg atgcgctccg gcggccagcg tggaggcagt caggcaaacc gcgatccgct
     2701 tggccgcaag cagggcgacc ggctcgacga tggcgagagc gtcaaggtcc cggacgaaat
     2761 cacggttcag cgggcgcgca caattctgga agagcttcgc aagagacttg gccagccgtc
     2821 acggccgccc attgagctcg attacctcga acgtctcgtc aagccgtatt agccttgtgc
     2881 tatccatcgg ctggctcgat atttgcagta tatcaggaac gaagagcgga aaggctaggg
     2941 gagcggcatg cagattgacg agctcgtcga caatttcagt tttctcgacg actgggagga
     3001 ccggtatcgc tatctgatcg agctcggccg cgcacttgac cccttgagtg acaccgaacg
     3061 cacaccggag acgaaagtgc ggggctgcgc aagccaggtt tggctgattt cgcacgcaaa
     3121 aggacaaggg ccggatgcgg ttctcgattt ccgaggcgac agcgatgcgc atatcgtgcg
     3181 cgggctcatc gccgttcttt tcgccttgta ttccggaagg acagcccggg agatccttac
     3241 aaccgatgag aaggccgtcc tcgccaagct gggcttgaac gaccatctca cgccccagcg
     3301 ctcgaacggt cttgcctcca tggtggcgcg gatcaagcgc gacgcgctcg cagcctccgg
     3361 cgcggagact acggcggttg tgcagtaaac ccttctcttt tctgttagcc caagcgaggc
     3421 tttgacgcga acgctgcaat gatattaaaa aagtaaggga tgccgcgtaa cttaggcgga
     3481 ccagcgtcgt ttgtttcggg tccgtttcgt gatgcagttt gtcttagacg ccctgccttc
     3541 cgctccttcc gt
//
LOCUS       DATMGX010000015         3218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_373383, whole genome shotgun sequence.
ACCESSION   DATMGX010000015 DATMGX010000000
VERSION     DATMGX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373383"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..315)
                     /locus_tag="VNR65_00245"
     CDS             complement(157..315)
                     /locus_tag="VNR65_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17132.1"
                     /translation="MISLYEIPNTALVAELAPDYDKRTTLSALHGIALALPNPLVERL
                     HSRALRSW"
     gene            complement(342..1079)
                     /locus_tag="VNR65_00250"
     CDS             complement(342..1079)
                     /locus_tag="VNR65_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ17133.1"
                     /translation="MTTERDRVAVVTGGSYGIGRDFVAELSRRGYRTIVCARDSQKPA
                     QLEHEIPGVETIVCDMSDLDSVARFADTILQKTPAIDLLVSNAGRASEFDLKADDLSG
                     IDVAGEINLNLAGPLKLILALLPALKKRPGSGIIVVTSGYALVPYPRMPIYSAAKAGL
                     RSFTKSLRREMKRFGVHVLELAPPLVDTPAVQHKKGRKISSAEVVRQALRGLDDKREE
                     VLPGQARLLPMLLRLAPRLTENMIAGR"
     gene            complement(1402..1866)
                     /locus_tag="VNR65_00255"
     CDS             complement(1402..1866)
                     /locus_tag="VNR65_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ17134.1"
                     /translation="MALAQAYSEIGNAGDCIVAVSCKKLLVQVVSSLVSLFGRGDRMV
                     ALHARADELLVAPDRRRLLVLIASELVINALRHAFPGRRTGLIDVCLHVDEGQAHLMI
                     SDDGVGYNGAEHGQGGKLLHSLTAMLNGTLCRSNSPAGGLQASVSFPVRPAL"
     gene            complement(2492..3052)
                     /locus_tag="VNR65_00260"
     CDS             complement(2492..3052)
                     /locus_tag="VNR65_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17135.1"
                     /translation="MRRVEGYAIVSEDGMLANAAGVMPDALKFEADRHFFERALEEVD
                     VVVHGRHSHEQQPHSHLRRRLILTRRIPAIAADPSNEKAFFWNPAGASFEQALAALGT
                     PNDRVGIVGGSDVFGMFLDRYDFFHLSRAPNVRLPGGRPVFPEVPRWTPEEVLAAHGL
                     EPGQSELLDLAKGLAVVSWQRTTNSG"
BASE COUNT          682 a          937 c          939 g          660 t
ORIGIN      
        1 atagagactt caaagggccg gatcaataag ttttggaata gcaagcgtat tcgggagaat
       61 ttgctcgcta ttgttcttac tcaaaacgcg cgcaaggtct ctctccggaa cgctttcgct
      121 acctcagttc ttgcaaggaa cgcgtgcgcg gcggacttac catgaccgca gcgccctcga
      181 atgcagacgt tcaacaagcg ggtttgggag tgctagcgca atcccgtgca acgccgagag
      241 ggtcgtgcgc ttgtcgtagt cgggcgcaag ctcggccacg agcgcggtgt tgggaatctc
      301 gtagaggctg atcatcagcc tcctgaattt tctgccagtc ttcaccgccc ggcaatcatg
      361 ttctctgtca agcgcggcgc gagccgcagc agcatcggca gcaaacgtgc ctgtccgggc
      421 agtacttcct cccgcttgtc atcgagtcca cgtagcgcct gccgcaccac ctcggcagac
      481 gatatcttac gccccttttt gtgctgcacg gcgggtgtat cgactaaggg cggcgccaac
      541 tcgagcacat gcacgccgaa acgcttcatt tcgcgccgga gcgatttcgt gaaactgcgt
      601 aagcccgctt tggcggccga atagatgggc atgcgcggat aaggcacgag cgcatagccg
      661 gacgtgacca caatgatgcc ggagccgggt cgcttcttca gcgcgggcag caaggcgagg
      721 atgagtttga gcggtccggc gagattgagg ttgatttcgc ctgccacatc gattcccgaa
      781 aggtcgtccg ctttgagatc gaattcgctg gcccgccctg cgttgctgac cagcaggtcg
      841 attgctggcg tcttttgcag gatcgtgtcc gcaaagcgcg cgactgagtc cagatcggac
      901 atatcgcaaa cgatggtctc gacgcccgga atctcgtgct cgagttgggc gggcttttgg
      961 ctgtcccgag cacacacaat ggtccggtag ccgcgtcgcg atagttcggc cacaaaatcg
     1021 cgcccgatac cgtagcttcc gcccgtgaca acggccaccc gatcgcgctc agtcgtcatg
     1081 atcaatgcca cctttccaat gcttgtctga gattgttcgt ggcgggccgc tgcgtacccc
     1141 gcacagaatt gtttgcctgg actctgagcc cagcgctcta aaatgcgcgg cgtaccgctt
     1201 caggaacctc ccgcacttcc gtggcaacgt caatggcttc cgagttgcca tagcgagccg
     1261 ccattatgat gcaatacagg atgttctagc cgtgaggcaa tgtgtgcgcc aactgatggc
     1321 gaacatcgta tatccctatt ttcatgtgaa gcgcaacgcg tagaattggg ccgctttgtt
     1381 ttcctctgcg cactacgttg cctacagcgc gggacgaacc gggaagctaa cggaagcctg
     1441 aaggccacct gctggagagt tggacctgca aagcgtgcca ttgagcatgg ccgttaagct
     1501 gtggaggagt tttccgcctt ggccatgttc cgcgccatta tatcccacac catcgtctga
     1561 aatcatcagg tgcgcttgcc cttcgtcaac gtggagacaa acgtcgatga gcccggttct
     1621 tcttcccgga aaggcgtgtc tgagcgcatt aataacaagt tcgcttgcga tgagcacgag
     1681 gaggcgacgc ctgtccggcg ccactaaaag ttcatcggcc ctggcgtgaa gagctaccat
     1741 tctatcgcct cgcccgaaca gtgagaccag actggagacc acctgcacca aaagtttctt
     1801 gcaggaaacc gccacaatac agtcaccggc attcccaatc tcggaatagg cttgggcaag
     1861 agccacagca gcctcaatcg ctgtggatcc gtggacaaga cgaagatgaa taacgttatg
     1921 cgcgagctga gctagattct tagcgcgatg cgccgcttca gccgagcaaa ccatttcgcc
     1981 gtgcttgcct gacgcaaatt ggctgagaac gaattctggc gtgaattgcg ggtggtgcgc
     2041 ccttctcggc tgactgggaa gagatagctt ttgcagcgaa tgagcgagcg gggatagggt
     2101 ttcgaactcc ggcaacggta tcgtcgcgca atcagttcgc gatactggtg tagactgcgc
     2161 cacgagcaca ttcttccaaa acgccagctc ccgagcaggt tgggacccga gccgatagca
     2221 ggaaggttaa gctgcggacg gcaatgctca tagactcgga atctactcag gcgcgccagc
     2281 gggcaggcgg gagggacgag gaaacccggt taggaggttt gtaaaaccgt tagggacaaa
     2341 aaaggtggta tacagggccg gaatcgaacc agcgagacgt agattttcag tatgccggcc
     2401 gtatccgaat cctgattccc gagtagagga cgagcgattg cggtatgttc agttgctcat
     2461 cgaccgcgat aggctcggga actacgcaag ctcatcccga atttgtcgtg cgctgccaac
     2521 tgacgactgc caaccccttt gccaaatcaa gcaactcgct ctggcctggc tcaagcccat
     2581 gggcggcaag gacttcctct ggggtccacc tgggcacctc cggaaagacc ggccgtccgc
     2641 cgggcagccg cacattgggc gcgcgcgaca agtggaaaaa atcataacga tcgagaaaca
     2701 tcccgaacac gtcagagccg ccgacgatgc caactctatc gttcggagtt ccaagcgcgg
     2761 ccagggcttg ctcgaacgaa gcgccggccg ggttccagaa gaacgccttt tcattcgatg
     2821 gatcggccgc gatggccgga atccgccgag tcagaatcaa tcggcggcgc aagtgcgaat
     2881 gcggctgctg ctcgtgagag tgacggccat gcaccaccac atcgacttct tcgagcgcgc
     2941 gctcgaagaa gtgccgatca gcctcgaact tgagcgcgtc cggcatgacc cccgccgcat
     3001 tcgcaagcat gccatcttcg gagacaattg cgtaaccctc gacgcgacgc ataagctgac
     3061 atactcccgt tcttgcagat caattgggtc acccgtgttt gtcgggcgca tgttttggct
     3121 gcactgacca ggccgctggc tcgtcgtcct tgtctgcttt ggccgccgcc gcagtcgcct
     3181 gcaccttgtc gggcgtatga tgggcgggcg cgtagatg
//
LOCUS       DATMGX010000016         6961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_474974, whole genome shotgun sequence.
ACCESSION   DATMGX010000016 DATMGX010000000
VERSION     DATMGX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474974"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VNR65_00265"
     CDS             complement(<1..334)
                     /locus_tag="VNR65_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17136.1"
                     /translation="MMMAPGGTREMCRVVLDEGADAVYVGVRGWSRRGSEHELTDDEI
                     RQTIDYAAARGKEVRVVVNTLPSSTEIPMFIERIQMYANWGAKGFMISDVGCMRLVHE
                     LSPHVAIHT"
     gene            complement(630..1613)
                     /locus_tag="VNR65_00270"
     CDS             complement(630..1613)
                     /locus_tag="VNR65_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ17137.1"
                     /translation="MNLHQLRIFCAIAQSSTLTQAAKQLGLAQPTLSQQLSKLEQKIG
                     AKLFDRGLNQMELTNAGRYLLRRAQVILHEVDEAQSALREFASGTRGIIRLAGLNSII
                     REVVPLAIERLSAQFPEVEFDIHEMAPAEVLDLLYARQVNIGLVAANSVGLSSGSFKE
                     FPVVRDPYVFAVPASIDLAAISDPDADLPPDQRKVLNSCIRFNFASQHSRQVEYWYQE
                     MLPSHRLFAQCRSYEVALSMVRAGLGVCLVPALAVYDNCCGSGGMKLYATDRPDRRTV
                     AVVPSQYQRIEPYSAFLEALRQAGSAVRMPPILSIPPFISRAVARRESADA"
     gene            complement(1781..2803)
                     /gene="hisC"
                     /locus_tag="VNR65_00275"
     CDS             complement(1781..2803)
                     /gene="hisC"
                     /locus_tag="VNR65_00275"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWJ17138.1"
                     /translation="MAEEVQVEKAVVDLSANENPLGPSPEVINAIARDVGTVHRYPDG
                     KGTALKAALAKKLGVESANIVAGNGSSEIFDMIAGTLLRGGGEAIIGWPSFPSYRAAV
                     QRAGGYVTLVPLRDYVYDLDEMASRASHRTRLVMLGNPNNPTGLALTHADLDRFLDRL
                     PHGCIVCLDEAYSEYVQSRDFPDSQRFIAEGRPVVVVHTLSKAYALAGLRVGYATASK
                     ALALSIDKHRQRFNTGRLAQIAAIAAIGDTDHLAKTIALNASGRKWLSARLSELGFFF
                     LPSEANFFLIKVGDAARVYQALKEGGVLVKQLDAFDLPEYIRVSVGTPDENARFIECL
                     QRAIRC"
     gene            3201..3593
                     /locus_tag="VNR65_00280"
     CDS             3201..3593
                     /locus_tag="VNR65_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ17139.1"
                     /translation="MKNSMLAAALIAALSMCGQAQADEVAKGKQIFDRTCGNCHPTQI
                     GLNKIGPSLWAIVGRPVASVPDYNYSGALISKRQDWRTWDEKRIDAYLVNPREVLHGV
                     KMYFKGLPEEKDRAAVLAYLKTLTWPDL"
     gene            complement(3937..5382)
                     /locus_tag="VNR65_00285"
     CDS             complement(3937..5382)
                     /locus_tag="VNR65_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWJ17140.1"
                     /translation="MTSTLMPWISPGVRNGYAGRKRGTILRASVAAFCFCIFGTGASA
                     AENAPLRLCADPDNLPFTSDKADNPGIYREIGKAIAQELNRPLTEVWYRTNFGKRATR
                     VTLLAKQCDFSVGLPGESDFMGPALIFSRPFMKAGYALVLKKPATVSSWADLNGKAIA
                     VQQNSTPQQVLATKDNITMVTELNPEDTVKAVASGAADAAFVWAPSAGYLNKTFYSDS
                     FNIIPVDGTGMQWPVAIAFGKRDGALRDAVNGALDRISAKIDDLKVKYGFPAAEPVTL
                     AEAATPANKPAKSAEQAPTPDKSAEKAAVVISEGQSSSEAPAAKSEDKLTAEVPAPAK
                     ADSAPAAAAEAPAPVKTEQQIATAVGAPAQAESAPSAAADAPQAAVENAALTPPPEPL
                     ASSNEDRVREGKEVFNGTCAHCHGPNAEQAERKIDLRLLHHRYKDSMEEMFFKTVTNG
                     RPSKGMPAWQDVFTQDQFVSILAFLKTVQAD"
     gene            complement(5495..>6961)
                     /locus_tag="VNR65_00290"
     CDS             complement(5495..>6961)
                     /locus_tag="VNR65_00290"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HWJ17141.1"
                     /translation="TPLVIGDTMYVTSSWGPKFVYALNAATGDKKWVYEPEIPDDVMQ
                     YGCCDVQNRGAAYADGKLFIGRLDGKLTALDAATGKELWTADVVDYKQGSVITSPPLV
                     VKNKVITGFGGGEYGARGSLQAYDVNTGKELWKTYTVPGPGETGNDSWKGDTWKHGGA
                     AAWLVGSYDPETNLVMFGTSNPSPWNASIRSTGTGDYGKLTNLYSASTVALDADTGQI
                     KWHLQGTPADSWDYDGVNEAVLADLTIGGKKVPVMMKADRNGFFFVANRKDGKVVSAE
                     KFVPTTWASKWDVATSRPVEDPARRASPGKPGKEACPNLIGGKNWQPMSFNPGTGLVY
                     IPSNNVCMDWAVSDVSYKKGVFFLGAEFPTLPGPGGYLGELIAWDPVAQKKAWGVKTD
                     LPFNGGTLTTGGNLVFWGDMHGFFRAFDAKEGKELWKMQLGSGIGPGAISYEVNGKQY
                     IAIVAGRSVSLPAFLGDIGKKLVAATPEAGQLFVFTVE"
BASE COUNT         1426 a         2037 c         2034 g         1464 t
ORIGIN      
        1 tggtgtggat ggcgacgtga ggcgagagtt catgaacaag gcgcatgcag ccgacatcgc
       61 tgatcatgaa gcccttcgcg ccccagtttg cgtacatctg gatgcgctca atgaacatcg
      121 gaatttcggt cgaacttggc agcgtattga cgacgacgcg gacttccttg ccgcgggcgg
      181 ctgcgtaatc gatggtttgg cggatttcgt cgtcggtcag ttcatgttcg gagccgcggc
      241 ggctccagcc ccggaccccg acatagacag catccgcccc ctcgtcgaga accacgcgac
      301 acatctccct ggtcccccca ggggccatca tcatgaccat cttgttccct tcccccggct
      361 cctggatcaa ccagcgccga acgcatcttg ctttttgggt acaaagatac cggcggcgca
      421 tgcgggccgt atatctatga gtgccattga tgtttcgaat aggagccgtc agaggaatcg
      481 actcctaccg tccactggaa tttccaatgc caagcatcgg attaaccaaa ttggctttgt
      541 ttcgtagaaa ccatataagt aaacatttaa gtagttttta ctcgcatcca gcgacggcca
      601 tgggcagagg cctcgtcacc cgattcgtat caggcgtccg cggattcgcg ccgggcaacc
      661 gcgcgactga tgaaaggagg aatcgacagg atcggcggca tacgcaccgc agacccagcc
      721 tgccgcagcg cttcgaggaa agcgctgtaa ggttcgatgc gctgatactg cgatggcacg
      781 accgccaccg ttcgccggtc gggccggtcg gtggcgtaga gtttcattcc gccggagccg
      841 cagcaattgt cgtacactgc gagcgctggg acgaggcata caccaagccc tgcgcgcacc
      901 atgctgagag ccacttcgta gctccggcat tgggcgaaga gccggtgaga gggcagcatc
      961 tcctgatacc agtactcaac ctgccgggaa tgctgggagg cgaaattgaa gcgtatgcag
     1021 ctattgagaa ccttgcgctg atcgggaggc agatcggcat cgggatcgct gatcgcagcc
     1081 agatcaatgc tggcgggcac ggcaaaaacg taagggtcgc gcaccaccgg gaattccttg
     1141 aaggagccgc tgctcaaacc aactgaattg gcggccacca agccgatatt cacctgccgc
     1201 gcgtaaagca gatccagcac ctcggctggc gccatttcat gaatgtcgaa ctcgacttcg
     1261 ggaaactgag cgctgaggcg ctcaatagcc agtggcacga cttcacggat gatcgaatta
     1321 agacctgcaa gccggatgat gccgcgcgtt cccgacgcga attcccgcag cgcgctttgc
     1381 gcctcatcca cctcgtgcag gattacctgc gcgcgccgta gcaaataccg gcccgcgttc
     1441 gtcagctcca tctgattgag accgcggtcg aaaagcttgg cgccgatttt ctgctccagc
     1501 ttcgataatt gctgcgacag tgtcggctgg gcgagcccga gttgcttggc cgcctgggtc
     1561 agcgttgacg attgagcaat cgcgcagaag atgcgcaact gatgcaagtt catgctggtc
     1621 gcttccctct gtgaagcctg tgcagcgaaa cagtctctaa aagaccgctg agagcgattt
     1681 tttccatggt tcgacaagct caccatgagg gttggcccgt tgataacact cggtctcatc
     1741 ctgagcctgt cgaaggatga agccgcggac tcaggctttt tcagcagcgg attgcccgtt
     1801 gcaggcactc gatgaaccta gcgttctcgt caggggtgcc gacgctgacc cgaatgtatt
     1861 cgggcagatc aaacgcatcg agctgtttaa ccagaacgcc gccctctttc aaagcttgat
     1921 agactcgcgc ggcatcgccc accttgatca gaaagaaatt ggcctcggac ggtagaaaga
     1981 agaaaccgag ttctgagaga cgcgccgaca gccatttgcg gccgcttgcg ttcagagcga
     2041 tggttttggc gagatggtct gtatcgccga tggcggcaat ggcggcgatc tgggccaggc
     2101 ggccggtgtt gaaccgctgg cgatgcttat cgatgctgag tgcgagagcc ttgcttgcgg
     2161 ttgcgtagcc aacgcgcaac cccgccaggg cataagcctt cgagagtgta tgcaccacca
     2221 cgaccgggcg cccctccgca ataaagcgct gggaatccgg aaagtctcgg ctctgcacat
     2281 attcgctata ggcctcgtca aggcaaacga tgcacccatg aggcaggcgg tcaagaaaac
     2341 ggtcgaggtc ggcgtgagta agagcgaggc cggttgggtt gttggggttg ccaagcatga
     2401 caaggcgcgt ccggtgactg gcgcgactgg ccatctcgtc gagatcgtag acatagtcac
     2461 gcaagggcac gagcgtcaca tatccgcctg ctcgctgaac cgctgcgcgg tagctcggaa
     2521 atgaaggcca gccaatgatc gcctcgcccc cgccgcgcaa gagcgtgccg gcgatcatgt
     2581 caaagatttc cgatgagccg ttgccagcaa cgatattcgc ggattcaacc ccgagcttct
     2641 tcgcgagcgc agccttcagt gctgtcccct tgccatcagg atagcgatga accgtcccca
     2701 catcgcgagc gattgcgttg attacctcag gcgatggccc gagggggttt tcattggctg
     2761 agagatcgac cacagccttt tcgacttgca cttcctccgc cacctcaatc cccaaatcca
     2821 aaagcctggc gaagcgggcg cgcgcatcaa aacgacgaag agaccatgct tgaatgccgt
     2881 ctgatgcccc aatcgcagcc tcggcactat gcaaaattgt gaatggattg tcagcagatg
     2941 aaaaccatgc ggctggtccg tccacgtcat tcggcccctc ttttggcggt catgaacgcg
     3001 gtcaaaagga cgcggatcca tcctgcttgt atggctgcaa gagagggatg atggttgtac
     3061 cttggtgcca ttgacggacc aaggtttgtg gcggatgtag atacgatgct tttctgttcg
     3121 tatcgggcac aaatccaaaa gaaccgtcga tacaaggttg gcaactcagc gcttattagg
     3181 gcgggaagca agagggtaac atgaagaatt ccatgctggc tgccgctttg attgcagcgc
     3241 tttcaatgtg cggacaagcg caggcggacg aagtcgcgaa gggtaagcag atcttcgacc
     3301 gtacttgcgg gaactgtcat ccaacacaaa tcgggttgaa caagatcggc ccctccctgt
     3361 gggctatcgt cgggcggcct gttgcgagcg tcccggatta caactattcg ggcgcgttga
     3421 tcagcaagcg ccaagactgg aggacatggg acgagaagcg catcgacgcc tatcttgtca
     3481 acccgcgcga agtcctgcat ggcgtgaaga tgtatttcaa gggccttccg gaagagaagg
     3541 accgcgcagc cgtcttagcc tatctgaaga cgctcacttg gccggacctt taaatcttgc
     3601 tttttagagt ttaacccttg cacgcggaag atgttgtaag gctgttatac acgcattagt
     3661 ctaggcttaa gcagagcaga cggggtacct gttctgctgg tttccaaaag ctgtaatgcg
     3721 tgtgtgaaac caagccccta gcgcacagta acgctagggg ctgttatcac cgtcatcgcc
     3781 tcatccggga agggcggcgt aacccgcgct cgcaccgagt gcgaccgata cggcttaatc
     3841 gaacatagtt tttgactgac ccgggagtgc ttacattgtc ctcgcgcgcg ctcggcgtcc
     3901 gatgacgatc cacttggcca gattagcaaa gttcgcttag tccgcttgca ccgttttcag
     3961 aaacgcgagt atcgaaacga actgatcttg cgtgaaaacg tcctgccaag ctggcatgcc
     4021 tttcgagggc cgcccgttcg tcacggtctt gaagaacatc tcctccatgc tatccttata
     4081 gcgatggtga agaaggcgaa ggtcgatctt gcgctccgcc tgttccgcgt tcggaccatg
     4141 gcaatgcgcg catgtgccgt tgaacacttc cttgccctcg cgaacccgat cctcattcga
     4201 ggaggctaag ggttccggag gcggcgttag tgcagcattc tcgactgcgg cctgaggcgc
     4261 atcggcagca gcggagggtg cgctttcggc ctgcgccgga gcccctaccg cggtcgcgat
     4321 ctgttgctcg gttttcaccg gcgcaggagc ctcagcagca gcggcaggcg cgctgtcggc
     4381 ctttgccggg gcgggaacct cggcggtcaa tttgtcttcg gatttcgctg cgggagcctc
     4441 gctcgaggat tgtccctcgg atatcacgac cgcggccttt tcagcggatt tgtcgggcgt
     4501 gggcgcttgt tctgcggatt tcgctggctt gttcgctggc gtggctgctt cggcaagagt
     4561 tacaggctca gcggcaggga aaccgtactt gaccttcaga tcgtctattt ttgcggaaat
     4621 ccggtcgagc gcaccgttga cggcatctct cagagcccca tcgcgctttc cgaaagctat
     4681 ggcgacaggc cattgcatgc cggtgccatc gaccgggatg atattgaagc tgtcggaata
     4741 gaaggtcttg ttcaagtaac cggcgcttgg cgcccaaacg aaggcggcat cggctgcgcc
     4801 gctcgcaacc gctttgaccg tgtcctccgg attgagctcc gtgaccatgg tgatattatc
     4861 cttggtcgcc agaacctgct gcggcgtcga gttttgttgg accgcgatcg ccttgccgtt
     4921 caagtcggcc cagctcgaca ccgtggccgg ctttttcagg acgagcgcat atcccgcctt
     4981 cataaaaggc cgtgaaaaga tcagggcggg ccccatgaaa tcggattcgc ctggaagccc
     5041 gaccgaaaag tcgcattgct tggcaagaag cgtcactcgc gtcgcgcgct tgccgaaatt
     5101 ggtccggtac cagacctccg tcagcgggcg gttgagttct tgcgcgatgg ccttgccgat
     5161 ctcgcgataa attcccggat tgtctgcttt gtcgctggtg aaagggagat tatcggggtc
     5221 tgcgcaaagg cgaagcggcg cgttctctgc ggcggaggca ccagttccaa agatgcaaaa
     5281 gcagaaggcc gcaacggatg ccctgaggat cgttcctctc ttccggcccg cataaccatt
     5341 cctcacgccc ggcgagatcc aaggcatcaa tgtcgaggtc atttccttcc ccagctcttg
     5401 tagatgttct gtggagttct ctttgaggaa ctcagctaca gactatacgg cggccgggaa
     5461 ggccgccgca actatcatgg attgagagca atctctactc aaccgtgaag acgaacagct
     5521 gaccagcctc gggggtcgcg gcaaccagtt tcttgccgat atcgccaaga aaggcgggaa
     5581 gcgagacgct tctgccggca acgatcgcga tatactgctt gccgttgacc tcgtagctga
     5641 tcgctcctgg cccaataccg gacccgagct gcatcttcca aagctctttg ccctctttcg
     5701 cgtcgaaggc gcggaaaaag ccgtgcatgt cgccccagaa tacgaggttt ccgcccgtcg
     5761 tcagcgttcc gccattgaac ggcaggtcgg tcttaacgcc ccaagccttc ttctgcgcaa
     5821 ccggatccca ggcgatcaac tctcccagat aaccaccggg tccaggcagc gtcggaaact
     5881 ccgcgccaag aaagaagaca cccttcttat aggagacgtc gcttacggcc caatccatgc
     5941 aaacattgtt cgatggaatg tagaccaggc ccgttcccgg attaaaggac atgggctgcc
     6001 agttcttgcc gccgatgagg ttcgggcagg cctccttgcc gggcttgccg gggctcgccc
     6061 gccgggcggg atcctcgacc ggcctcgacg tagcaacgtc ccactttgaa gcccaggtcg
     6121 tgggcacgaa tttttcggcg gaaaccacct ttccgtcctt gcggttggcg acaaagaaga
     6181 agccgttgcg gtcggccttc atcatcaccg gaactttttt gccgccgatc gtgaggtcgg
     6241 cgagcaccgc ttcgttgacg ccgtcatagt cccaggaatc ggcgggagtg ccttgcagat
     6301 gccacttgat ctgcccggta tccgcatcga gcgccaccgt ggaggcggaa tagaggttgg
     6361 tcagcttgcc gtaatccccc gtgccggtgc tcctgatcga cgcattccag gggctcggat
     6421 tgctggttcc gaacatgacc agattggttt cgggatcgta cgagccgacg agccaggccg
     6481 cagcgccgcc atgcttccag gtgtcaccct tccagctgtc attgccggtc tcgcccggac
     6541 caggaaccgt gtaggtcttc caaagttcct tgccggtgtt cacgtcgtag gcctgcaggc
     6601 tgccacgtgc gccatactcg ccgccgccga aacctgtaat aaccttgttc tttaccacga
     6661 gcggcggaga ggtgatgacc gacccttgct tgtagtccac cacatccgcg gtccaaagct
     6721 ccttgcccgt ggctgcatcg agcgcggtga gcttgccatc caggcggccg ataaagagct
     6781 tgccatcggc gtaggccgcg ccgcgatttt gcacgtcgca gcagccatat tgcatgacgt
     6841 catcggggat ttcaggctcg tagacccact tcttgtcgcc cgtcgccgca ttcaaggcgt
     6901 agacgaattt cggcccccag gacgacgtga catacatcgt gtcgccgata acgagcgggg
     6961 t
//
LOCUS       DATMGX010000017         2789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_26223, whole genome shotgun sequence.
ACCESSION   DATMGX010000017 DATMGX010000000
VERSION     DATMGX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26223"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..951
                     /locus_tag="VNR65_00295"
     CDS             196..951
                     /locus_tag="VNR65_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ17142.1"
                     /translation="MHGLRARCKNCQASCDGICAVLGNEARADLLKISRKKTVPAHQA
                     IFRDGDLSDNYYNITSGIVKLVKTLADGHQHIVGLLYPADFMGQSLNKHHTYAAESAT
                     DVELCAYPRAPFEHFLISHPELERQIFHATIRELDLCRDWTLLLGRKCSYERVAGFLL
                     MMARRSASKGLDKPKNYVHFELPFTRAEMADYLGLTLETVSRQFSQLKKKEVIALPSS
                     RDIIIPDIELLSLVAQIESCSQDFDERQSRAVA"
     gene            995..1834
                     /locus_tag="VNR65_00300"
     CDS             995..1834
                     /locus_tag="VNR65_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWJ17143.1"
                     /translation="MSIKTILVYLPSEKNASIILEPALKIAVERSAHVIGLHLTPDLP
                     VYGEFPAEVSEEVIVRLQKAGKDAAAGARRVFDEWVKKSPVTHEWRCFTASYAAGTDL
                     IAQQGRTADLIVCGKPSDDIPDAWSDFSETALIRSGRPVLIVPGTKPLKTIGEHVVIA
                     WNDTREAARAVFDSVELLKAASTVRAVTLIEKESQRTAAEALGADLVANLSRHGVTAS
                     LDVSYAGSGGAGEAILSRLLEEGCDLLIMGGYSRSRFREMIFGGASRDILRDTWVPTL
                     LSH"
     gene            1922..2308
                     /locus_tag="VNR65_00305"
     CDS             1922..2308
                     /locus_tag="VNR65_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17144.1"
                     /translation="MTLRKIRLELARDREHPEGSKRHGYEFVGPLDEEGRLDPVLWKK
                     YRERCRVRRFWQNEDDELGHLVRKPGGHWAFHYDIHGDADDDESGYRLGNHKFAVGEY
                     VSIREHEDDVMRTFRVVRVEPLPPPA"
     gene            complement(2424..>2789)
                     /locus_tag="VNR65_00310"
     CDS             complement(2424..>2789)
                     /locus_tag="VNR65_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17145.1"
                     /translation="IFVVGTETDHVAPWRSVYQINVLADTDVTFLLTSGGHNAGIVSE
                     PGRKNRAYRVATSKHRAPYIDPDTWLQRNKPVQGSWWPEWMQWLKSGSNEFVAPPPMG
                     APEAGYAPIGDAPGTYVLE"
BASE COUNT          604 a          803 c          799 g          583 t
ORIGIN      
        1 gccgcgatag agaaaactta ggcgctgcaa tctgttacat cgatggcggt gcccaaccat
       61 tgtctgttta gacgggtaaa aaaaatttcc ggaaatatct ctacaccgga ggaaagctta
      121 cagcgtgcct aattcgtttg attgaataca accccggtgg tatgctcagc gcttgtgcag
      181 ttatgggaga acccgatgca cggccttcgc gcgcgctgca aaaactgcca ggccagctgc
      241 gacggtatct gcgccgtcct gggcaatgag gcgcgagccg atttactaaa gatttcccgg
      301 aaaaaaactg taccagccca tcaggccatt tttcgagatg gcgacttatc agacaattac
      361 tataacatta cctcgggaat tgtgaagctc gtcaagacgc tcgcggatgg ccaccagcat
      421 attgtcggcc tactttaccc ggccgacttc atggggcaat cgctcaacaa gcaccacacc
      481 tacgcagcgg agtcggcgac cgatgtcgaa ctctgcgcct acccgagagc gcccttcgaa
      541 cacttcttga taagccatcc agagcttgag cgccagattt tccatgcaac cattcgcgaa
      601 ctggacctat gccgcgactg gacgcttctt cttggccgca agtgctccta tgagcgcgta
      661 gcagggttcc tgctcatgat ggcgaggcgc tccgccagca aaggcttgga caagccgaaa
      721 aactatgtgc acttcgaact gccattcacg cgcgcggaga tggcggatta cctcggtctc
      781 acgctcgaaa ccgtgagcag gcaattcagc cagctcaaga agaaggaagt tatcgccctg
      841 ccttcgagcc gggatattat tattcctgat atcgagcttc ttagcctagt cgcccaaata
      901 gaaagttgtt cccaagactt tgatgaacgt caaagccggg ctgttgcctg atacgtagca
      961 tgaaccatcg atgggcaatg ctggagctct taccatgtct atcaaaacaa ttttggttta
     1021 tttaccctcc gagaagaacg catcaatcat tctcgagccg gcgctaaaga tagcggtcga
     1081 gagaagtgct catgttatcg gattgcacct cactcccgat ctgcccgttt atggcgagtt
     1141 tcccgccgag gtttccgagg aagtgatcgt acgcctccag aaggcaggca aggatgccgc
     1201 tgcgggcgcg agacgggttt tcgatgaatg ggtgaagaaa agccccgtca cgcatgaatg
     1261 gcgctgcttc accgcatcct atgccgcggg caccgatctg atcgctcagc aagggcgcac
     1321 cgcagatttg atcgtgtgcg gaaagcccag cgacgacatt ccggacgcct ggagcgactt
     1381 ttccgagacg gcgctcattc gcagcgggcg tccggtgctg atcgtccctg gaacaaagcc
     1441 cctgaagacc atcggcgaac acgtcgttat cgcctggaac gatacgcggg aggccgcgcg
     1501 ggccgtgttc gattccgtgg agcttctcaa ggccgcttcc accgttcgcg ccgtcaccct
     1561 catcgagaag gaaagccagc gcacggcggc ggaagctctg ggggccgacc tggttgccaa
     1621 tctcagccgc cacggcgtta ccgcatccct tgacgtctca tacgcaggca gcggtggcgc
     1681 cggagaagca atcctgtcgc ggctgctcga agaaggctgc gaccttctga tcatgggcgg
     1741 atatagccgg tcgagattcc gcgaaatgat ttttggcggc gcaagccggg acattctgcg
     1801 cgacacttgg gtgccaacgt tgttgtcaca ttagagcctc gtgcgtcttt atcggaaaac
     1861 cgcttcacac ttttccggac gcactctagg cggtccgcgc cttttgcttt ggagatcggt
     1921 catgacgcta agaaaaataa gattggaact ggcgagagat cgtgagcatc ccgagggcag
     1981 caaacgccac ggctatgagt tcgtcggtcc attggacgaa gaggggcggc tcgacccggt
     2041 cctctggaaa aaatatcgcg aacggtgccg tgtgcgtcgg ttctggcaga atgaggatga
     2101 cgaactcggg catctcgtga gaaagcccgg cggccactgg gcttttcatt acgacatcca
     2161 cggcgatgcc gatgacgatg agagtggcta ccgcctgggc aatcacaaat tcgcggtggg
     2221 ggaatacgtt tcgatccgcg agcacgagga tgacgtgatg cgaaccttcc gcgtggtccg
     2281 tgtcgaaccg ctcccgccgc ctgcttgagc cgcgccattg attaccccgg ctccgttcac
     2341 ttggtcccgg gccggatttt cgaatagcgg ccgatggctt caggacagcg ccctggagcc
     2401 atcacggctc ccaggcaaaa ccgtcattct aaaacgtaag tgccaggggc gtcgccaatc
     2461 ggcgcatatc ctgcctcggg cgcacccatt ggcggcggcg cgacgaactc gttcgatccg
     2521 gacttgagcc attgcatcca ctcaggccac caggatcctt gcactggctt gttcctttgc
     2581 agccatgtgt ccgggtcgat gtacggggcg cgatgttttg acgtggccac gcggtaggct
     2641 cggttcttgc gccccggctc tgacacgatg ccggcattgt gcccgccgct cgtgagaaga
     2701 aatgtaacgt cagtatcggc gagcacgttg atctggtaca ccgagcgcca cggtgcgacg
     2761 tgatccgtct cggtgccgac gacgaaaat
//
LOCUS       DATMGX010000018         3403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_26175, whole genome shotgun sequence.
ACCESSION   DATMGX010000018 DATMGX010000000
VERSION     DATMGX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26175"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VNR65_00315"
     CDS             <1..252
                     /locus_tag="VNR65_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17146.1"
                     /translation="SPVGQRLVCRVPQGHYQTTTMIAAVRLQGPQAPWLFEGPMDGEL
                     FLAWVKQGLVPVLRRDDVVIGWRQLKFSSDDYWFVGGLG"
     gene            305..1798
                     /gene="istA"
                     /locus_tag="VNR65_00320"
     CDS             305..1798
                     /gene="istA"
                     /locus_tag="VNR65_00320"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HWJ17147.1"
                     /translation="MITKQQYRKLMNEYQATGNVTNSALKAAMSRPTARKYVTAVQPP
                     AELQAKHGWRTRKDPLVAIWPQAEKLLVEAPDLEAKALFEHLWERSPWAVQEKHLRTF
                     QRRVKLWRLAHGADQEVFFPQDWETGRALQLDWTNADELGITIAGEPYPHLLCHCVLP
                     HSNWEWATRCRSESLLSLRQGLQAGLHRLGKVPRELRVDNSSAATHRIGGGETARTFN
                     EPFASLCSHYGMVPRTIGIGCPNENGDVESSNGHLKRRLRQHLLLRGSREFAAETDYE
                     HFLERVLERSNQGRREKLAAELAVMTALPPTRLSEYDEVICRVGSASTIRVKKVGYSV
                     PARLIGQELKVEVYERELKLYSGRELLLSLPRHTGNQGMVLDYRHVIDHLLRKPGSFE
                     RYRYREQLFPSRLLRTAYDHLVAQQGARRGTVEYLRLLKLASEVGASGVEVMLADYLS
                     PPYPAWSVAELRGVLQPSPRSPIELDELQPEWRSYDALLSQEVTCVN"
     gene            1788..2636
                     /gene="istB"
                     /locus_tag="VNR65_00325"
     CDS             1788..2636
                     /gene="istB"
                     /locus_tag="VNR65_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HWJ17148.1"
                     /translation="MSTDGLAGLLRSFSLPTMAAMWAESVARAERENWGYKRLLQHLC
                     ESEEQDRRERKMQRLLKASCLPDGKTLGNLDEKLLPTKIRRLLPTLLEGHFVERAENI
                     LAFGLPGRGKSHFLAAVGRELILRHRYPVLFTPTFKLVQQLLVAKQQLRLDSELKRLD
                     RYPIIILDDLGYVQQDRQEMEVLFTFLAERYERRSVLISSNLVFSKWDQIFKDPMTTM
                     AAIDRLVHHAMILEFNGESVRAQKAQGRDKPQVNPPGDPFGSTPSSAPDGAKDSVPPK
                     GSPQQK"
     gene            2715..3329
                     /locus_tag="VNR65_00330"
     CDS             2715..3329
                     /locus_tag="VNR65_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ17149.1"
                     /translation="MTKWDLLPAQITVRILRFTTTIRGFRSRRVTLVTTLLDPKLYPA
                     EELVALYARRWRLELCLRDLKTTMGMEPLRCKTPEMAEKELLAYLVAHNLVRCLMAEA
                     VATHRVDLEQVSFKGSLDALRQFSDAISRAPNRKLRRQLWEELLLNLARDLVPLRPNR
                     TEPRALKRRPKPYPLLNKPRRKFVEISHRSRYWKGRPRNYRGLN"
BASE COUNT          702 a          928 c         1105 g          668 t
ORIGIN      
        1 agcccggttg gccagcggct ggtctgtcgc gtgccgcagg gacactatca gacgaccacc
       61 atgattgcgg ccgtgcggct ccaggggccg caagcccctt ggctgtttga ggggccgatg
      121 gatggcgaac tgtttctggc ctgggtaaaa caggggctgg tgccggtgct gcgacgcgac
      181 gatgtggtca ttggctggcg acaattaaaa ttttccagtg atgattattg gtttgtcggt
      241 ggtttgggat gatggcgcag tggtttttgc cgcgaccgga gggtcgcggc ggggactgct
      301 gcgaatgatc accaaacaac aataccgaaa actgatgaat gaatatcaag ccactggcaa
      361 cgtaacgaac agcgcgctga aagcggccat gagccgaccg acggcgcgga agtatgtgac
      421 ggcggtgcag ccgccggcgg aactgcaagc caagcacggt tggcgaacgc ggaaagatcc
      481 gctggtggcg atttggccgc aagctgagaa gctgctggtg gaagccccgg atttggaggc
      541 caaggcgctg tttgaacatt tatgggagcg ttcgccctgg gcggtgcagg aaaagcatct
      601 acggacattt caacggcggg tcaagctgtg gcggctggcg catggggcgg atcaggaagt
      661 gttctttccg caggactggg agaccgggcg tgcgttgcag ttggattgga ccaacgccga
      721 cgagttgggc atcacgattg cgggcgagcc ttacccgcat ctgctttgtc actgtgtcct
      781 gccgcattcc aactgggaat gggcgacccg ctgccggtcc gaatcgttat tgtcgttacg
      841 gcagggcttg caggctggct tgcatcggct gggcaaagtg ccgcgggagt tgcgggtgga
      901 caacagcagt gcggcgaccc atcggatcgg cggcggtgag acggcgcgaa cattcaatga
      961 gccgtttgcc tcgttgtgtt cgcattatgg catggtgccc cggaccatcg gcatcggctg
     1021 tcccaatgag aacggcgatg tggagtcgag caacggccat ttgaagcgcc ggctgcgcca
     1081 gcatctgttg ctgcggggca gccgggagtt cgcggcggaa accgactacg aacatttttt
     1141 ggaacgggtg ctggagcgca gcaatcaagg ccggcgggaa aagttggcgg cggagttggc
     1201 ggtgatgacg gcgttgccgc ccacgcggct gagcgaatac gacgaggtga tttgccgcgt
     1261 cggctcggcc agcacgatcc gggtcaagaa agtgggctac tcggtgccgg cgcggctgat
     1321 tggccaggaa ctcaaagtgg aagtttatga gcgggaactc aaactctaca gcggtcggga
     1381 actgttgctg agtttgcccc ggcacacggg caaccagggg atggtgctgg attaccggca
     1441 tgtgattgat catctgttgc gcaagcccgg gtcgtttgaa aggtatcggt atcgggagca
     1501 gctgtttccc agccggctgt tgcggacggc ttacgatcac ctggtcgccc aacagggagc
     1561 gcgtcgcggc acggtggaat atctgcggct gctcaagctg gccagcgagg tgggcgcgag
     1621 cggcgtcgaa gtaatgttgg ccgactacct gagcccgccg tatccggcct ggagtgtcgc
     1681 ggagttgcgg ggcgtgttgc aacccagccc gcgctcaccg attgagctgg acgagttgca
     1741 gccggagtgg cgttcgtatg atgcgttgct gagtcaggag gtgacctgtg tcaactgatg
     1801 gcctggcggg attgttgcgc tcgttctcgt tgcccaccat ggcggcgatg tgggcggaga
     1861 gtgtcgcgcg ggccgagcgg gagaactggg gttacaaacg tctgctccaa cacttgtgcg
     1921 aaagcgagga gcaggatcgg cgcgaacgca aaatgcagcg gctgctcaaa gcgtcctgcc
     1981 tgcccgacgg caagacgctg gggaacctcg atgaaaaact cttgccgacc aaaatccgac
     2041 ggttgctgcc gacgttgctg gaaggccact ttgtcgaacg ggcggaaaat attttggcgt
     2101 tcggactgcc cggccgcggc aagtcgcatt ttttggccgc cgtggggcgc gaattgatcc
     2161 tgcgtcaccg ttacccggtg ctgtttacgc cgacgttcaa actggtgcag cagttgttgg
     2221 tcgccaagca gcaactacgt ttggacagtg aactcaagcg cttggaccgc tatccaataa
     2281 ttattttgga cgatttagga tatgtgcaac aggatcgcca ggagatggaa gtgttgttca
     2341 cgttcttggc ggagcgctac gagcggcgca gcgtgctgat cagcagcaac ctggtgttct
     2401 cgaaatggga ccagattttt aaagacccga tgaccacgat ggccgcgatt gaccggctgg
     2461 tgcatcacgc catgatttta gagtttaacg gcgagagcgt gcgcgcgcag aaggcgcagg
     2521 ggcgagacaa gcctcaggtc aacccgcccg gagacccctt cggttccact ccgtcttcgg
     2581 cacctgacgg tgccaaagac tccgttccac cgaaggggtc tccccaacaa aaataatcct
     2641 ggagcacaga tgaagaaatt aaccccaagc taccggcaaa taataatcgt cgccggataa
     2701 aggaatgatt gtcgttgacc aagtgggatt tgctgccggc ccaaatcacc gtgcgcatcc
     2761 tccgtttcac caccacgatc cggggatttc gcagccgccg cgtcacactg gtgaccacac
     2821 tgctggatcc caaactctac ccagccgagg aactggtggc gctctatgcc cggcgctgga
     2881 ggttggaact gtgcctgcgg gatctcaaga cgacaatggg catggaacca ctgcgttgta
     2941 aaactccaga gatggcggag aaagaactgt tggcttacct ggtcgctcac aacctcgttc
     3001 gctgtttgat ggccgaagcc gtggcaactc accgggtgga cttggaacaa gtcagtttca
     3061 agggaagtct cgacgcgttg cgccagttca gcgacgccat cagccgggca cccaatcgca
     3121 aactgcgccg ccaactatgg gaggaattgt tgctgaactt ggcgcgcgat ttggtgcccc
     3181 tgcgacccaa ccgcaccgaa ccgcgcgccc tcaaacgccg ccccaagccc tatcccttgc
     3241 tcaataaacc ccgccgcaaa ttcgtggaaa tctcccaccg cagccgctac tggaaaggca
     3301 gaccccgcaa ttatcgaggc cttaactaac cgccattcgg gactgaccct tttcctcatt
     3361 gcgattgaac cgcgcgataa aaccgcgcgg gcggaacgat acc
//
LOCUS       DATMGX010000019         2299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_26038, whole genome shotgun sequence.
ACCESSION   DATMGX010000019 DATMGX010000000
VERSION     DATMGX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26038"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1440
                     /locus_tag="VNR65_00335"
     CDS             370..1440
                     /locus_tag="VNR65_00335"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HWJ17150.1"
                     /translation="MHSGWYDRRIRQVRDLPCGDTRIFLEIEVRRVRCRSCGKVKRER
                     LDFLADNPLYTKRFAHYVGQRCRQATIKDIAEELKLDWDTVKTLEKQYMQAQLARAGR
                     PGPKAIGIDEISIRKGHSYRIVVSDLIRRRPIWFGGEDRSEASMAQFYDWLGQKKSHG
                     IRLAVMDMWKPFRNTTRERAPQAAVLFDKFHIMRHLGEALDKVRKTEYARVSGKGRRF
                     IKGQKYTLLARHENLSLEGKRSLTLLLAANKRLNTAYVLKESFGQLWDYEREGWARRF
                     FENWRASLKWQRLAPYEKFAEMIDRHWDGIAAYCKPENKVSLGFVEGLNNKIRVIQRR
                     AYGLRDQEYLRLKVLTCMLPAL"
     gene            complement(2057..>2299)
                     /locus_tag="VNR65_00340"
     CDS             complement(2057..>2299)
                     /locus_tag="VNR65_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ17151.1"
                     /translation="FFVLNPFFILSGFSFPISSMPVVLQWFTYANPLRYYLVVIRGTF
                     LKGVGVDVLWPDLVAMATIASVLLAISILRFRKSLE"
BASE COUNT          524 a          618 c          692 g          465 t
ORIGIN      
        1 actctcgttg ggcttttgga aaagcctttg ctgtgcttcg tgcaacgccg ccgccccaaa
       61 cccgtgcttg atcgtttacc agctggcaga taattttggc tcatcaggaa atcgagtgga
      121 tgaatctaat gggaaatgtg gcttgaagcg acatggatat tgggttttcg aggtttccta
      181 aggtggttgg atcgggcatc attttccgat ggccaactca tcgaagcgac agcggcgcct
      241 gtgggacacg tacctgttcc ccggctttcg cccccaaccc acggtgcgcg gtgtcttcgg
      301 tgatccgaag gcgcgcatca tcacgctcgc ccggcgcgca aaaaaacggt ttgcgggtgc
      361 tgcgggcgaa ttcattcggg ttggtacgac cggcgtatac ggcaggttcg cgatctgccg
      421 tgcggcgaca cgcgcatctt cctggagatt gaggtccggc gggtgcgatg ccgcagttgc
      481 ggcaaggtga agcgcgagcg gctcgatttc ctggcggaca acccgctcta tacgaagcgc
      541 tttgcccatt acgtgggcca gcgctgtcgg caagcgacga tcaaggacat tgccgaggag
      601 ctgaagctcg actgggatac ggtcaagacg ttggagaagc agtacatgca agcccaactc
      661 gcccgcgcgg gcaggccggg gcccaaggcg atcggcatcg atgagatctc gatccgcaag
      721 ggccatagct accgtatcgt ggtgagcgac ctgatccgcc ggcggccgat ctggttcggc
      781 ggggaggatc gctcggaggc gagcatggcg cagttctacg actggctggg ccagaagaag
      841 agccacggca ttcgcctggc ggtgatggac atgtggaagc cgtttcgcaa cacgacgcgc
      901 gaacgggcgc cgcaggcggc ggtcctgttc gacaaattcc acatcatgcg ccatctgggc
      961 gaggcgctcg acaaggtccg caagaccgaa tacgcgcggg tcagcggcaa aggtcggcgc
     1021 ttcatcaagg gacagaaata cacgctgctc gcacgccacg agaaccttag cctggagggc
     1081 aagagatcgc tcacgctgct gctggcggcg aataagcggc tgaacacggc ctacgttctg
     1141 aaggaatcct tcggccagct ttgggactac gagcgcgagg gctgggcgcg gcgcttcttc
     1201 gagaactggc gcgcgagctt gaagtggcag cggctcgcac cgtatgaaaa gttcgccgag
     1261 atgatcgatc ggcattggga tggcatcgcc gcctactgca agccggaaaa caaggtctcg
     1321 ctcggcttcg tcgagggcct caacaacaag atccgcgtca tccagcgccg cgcctacggt
     1381 ctacgcgacc aggaatatct gcggctcaag gtgctcacat gcatgcttcc agccctctga
     1441 agttgccgaa aacacccact cgatttcctg aagacccaga aattagtccg cagattggcg
     1501 cggctgtgtt ggctggctga tcgaagacgc ttgacgtcgt aggctgcgtt ggcgcgatgg
     1561 aagatgagga taaacataaa ttgcgcaatc attgcccatc gcgctgatac gttcattttg
     1621 gtcgcagttt cgtcgttgac gtgcgaaagg ccaataatga cgccaattag catgaagagg
     1681 atggcgagct tgggactcat tttgcttgcc tcttaagcca gttctcctcg cctcgcgtac
     1741 agtgcagcgg caaattgctt ttttaaacaa ataggtgcgg tatcggcttt agtaaggcgc
     1801 acacgcaatg gcgcagctag ttaaaaatgc atgatctcga ttgacccaga tcaaggcggc
     1861 tgcctttagc atctggttca aaaacaaatt gctgagtcaa gaacaccttg cgactttgct
     1921 aagtcacaaa gtaaaaagtg cttgagctag ctcggccccc cccccgcgtc attgcgaacc
     1981 ttcgccacgc cgaggcattt taggctattt ctcaaacgat acctgaatga atgtaggcgc
     2041 acaattgatg cgccattcac tcaagagatt ttcggaaacg aaggatgctg atcgccagga
     2101 gaacgctggc gatagtcgcc attgcgacca gatccggcca tagcacatcc actccgacac
     2161 ctttgagaaa ggtgccgcgg atgactacga ggtagtaccg caatgggttc gcgtaggtga
     2221 accattgcaa tacgactggc atgctggaga tggggaagct gaatccagaa aggataaaaa
     2281 agggattgag cacgaaaaa
//
LOCUS       DATMGX010000020         2353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_372721, whole genome shotgun sequence.
ACCESSION   DATMGX010000020 DATMGX010000000
VERSION     DATMGX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372721"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..1781
                     /gene="tldD"
                     /locus_tag="VNR65_00345"
     CDS             354..1781
                     /gene="tldD"
                     /locus_tag="VNR65_00345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HWJ17152.1"
                     /translation="MDEKFFFERYGMTQADVERYLSAALSAGGDFADLYFEYQTSTSV
                     SLDESMVKSATQGISVGCGVRVLSGERTGYAYTDDLSPQRLLHAARTAALIASGPAKQ
                     PVVGLTNKPAHNLYPVPLPSVETEVLAKVDLVQRADKAARNYDPRIHEVRVSYADELR
                     LILVIGSDGSFAEDAQPLARMSVFCIAKDEVAGSARGTAGGGGRVALDYFQGEASPEH
                     FAKEAGRQAIIQLDAREAPAGEMEVVLGPGWPGILLHEAIGHGLEADFNRKKTSAFSG
                     MLGRRVASDKCTVVDNGTMPGRRGSLNVDDEGNQTENTVLIENGILKAYLSDKLSSRL
                     MGIPNTGNGRRESYQHIPMPRMTNTYMLAGQDSPEDIIKSVKYGVYAANFGGGQVDIT
                     NGKFVFSASEAYLIENGIITSPLKNATLIGNGPDVLTRVSMVGSDLQLDEGVGTCGKD
                     GQSVPVGVGIPTLKVDRLTVGGTGG"
     gene            1983..>2353
                     /locus_tag="VNR65_00350"
     CDS             1983..>2353
                     /locus_tag="VNR65_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HWJ17153.1"
                     /translation="MTVSAQESPGAAPTASTDLKEIAADVVRRAMQGGATAAEAVAMD
                     GSEFSTVVRLGEVETLKESGSKAIGVRVFFGQRAASTYSSDLTPEGVKQLVSSALALA
                     KVTSEDPFAGIPEPAQLGKLEG"
BASE COUNT          467 a          711 c          739 g          436 t
ORIGIN      
        1 aaggggcgca ccttcagggc ctgccctgag cccgtcgaag ggtgcgccgg caagtccttt
       61 tattcttgtc gtcccgagcc ggcggaccgg ggtccccaac gagcgccccg ccgcagcggg
      121 cttgttgggg taaagttcag ccggcgaggg aatctacttt tcgcaccttc tcccaagctg
      181 tgaagccgtg ccctttcaaa acacgactta tctcattccc ttcacgggaa agggtaattc
      241 aaccatgagc ataccgcatc tccaacggca cagcgctgtg tgataatcaa aggttcggac
      301 ctcggccgtc aggcgcgtct tctccggcgg cggggcatcc aatgagtgtg actatggacg
      361 agaagttctt tttcgagcgc tacggaatga cgcaagctga tgtcgaacgc tacctctccg
      421 cggcactctc cgctggcggc gacttcgccg acctttattt tgaataccaa acctccacct
      481 cggtttcgct tgatgagagc atggtgaagt cggccacgca aggcatttcg gtgggctgcg
      541 gagtgcgggt gttgtcgggg gaacgcaccg gttacgccta caccgacgac ctctcgccgc
      601 agcgactcct gcacgccgcc aggactgcgg cgcttatcgc cagcggtccg gcgaagcagc
      661 cggtagtggg cctgactaac aaacccgcgc acaatcttta tcctgtgccg ctaccctccg
      721 tggaaaccga ggtactggcc aaggttgacc tggtgcagcg tgccgataag gcggcgcgca
      781 actatgatcc gcgcattcac gaagtgcgcg tgagctacgc tgacgagctg cgtctcatcc
      841 tggtgattgg ttccgacggc agctttgccg aggatgccca gcccctggcg cgcatgagcg
      901 tcttctgcat cgccaaggac gaggttgccg gctcggcgcg cggcacggct ggtggcggcg
      961 gccgcgtagc gttggattat ttccagggcg aggcgtcgcc tgagcacttc gccaaggaag
     1021 cgggacggca ggccatcatt cagctcgacg cgcgcgaggc gccggccggt gaaatggaag
     1081 tggtgctcgg tccggggtgg cccgggatcc tgctgcacga agccatcggc cacgggctcg
     1141 aagccgattt caatcgcaag aagacttctg cattctcggg aatgctgggc cgccgcgtcg
     1201 ccagcgacaa atgcaccgtg gtggacaacg gcaccatgcc cggccgccgt ggctcgctca
     1261 acgtggacga cgaaggcaac cagacggaga acacggtgct catcgagaac ggcatcctca
     1321 aggcctatct ctccgacaag ttgtcgtcgc gcctgatggg cattcccaac accggcaacg
     1381 gccgtcgcga gagctatcag cacatcccca tgccgcgtat gaccaacact tacatgctcg
     1441 ccgggcagga ctcgccggaa gacattatta agtccgtgaa gtacggcgtt tacgcggcga
     1501 acttcggcgg cgggcaggtg gacatcacca acggcaagtt cgtcttctcc gcatcggaag
     1561 cctacttgat cgagaacggt atcattactt cgccgctgaa gaacgcgacg ctcatcggga
     1621 acgggccgga tgtgctgacc cgagtctcga tggtcggcag cgatctgcaa ctcgacgagg
     1681 gtgtcgggac ctgtggcaag gatggacagt cggtgccagt tggtgtgggc attcccacgc
     1741 tgaaggtaga ccggctcacc gtcggtggga ctggcggata aagcgtctct ggagattccg
     1801 atctcatttc gatttgcagg aaacacacag aaaacagccc cgaaggggcg aaagagctta
     1861 gcccagcgct tcagcgctgg gtgaagtgag aagtagactg agtcccgtag ggacggcaga
     1921 gggatctgct tttcaccctt actgtacgac attgtgacac tctgacaacg taggactaat
     1981 tcatgactgt ttctgctcaa gaaagccccg gcgccgcgcc gaccgcatcc accgatctga
     2041 aggaaattgc cgccgatgtg gtccgccgcg ctatgcaggg cggggcgacc gcggccgaag
     2101 ctgtcgccat ggatggcagt gagttctcca ccgtcgtgcg tctcggcgaa gtggagacgc
     2161 tgaaggagtc cggctccaag gccatcggcg tgcgcgtctt tttcggacag cgcgccgcca
     2221 gcacctactc cagcgacctg acgcccgaag gagtgaagca gttggtgagc tcggcattgg
     2281 cgctggcgaa ggtcaccagc gaagacccct tcgccggaat tcccgagccg gcgcaactgg
     2341 gcaaactgga agg
//
LOCUS       DATMGX010000021         2314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_474328, whole genome shotgun sequence.
ACCESSION   DATMGX010000021 DATMGX010000000
VERSION     DATMGX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474328"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="VNR65_00355"
     CDS             complement(<1..1358)
                     /locus_tag="VNR65_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWJ17154.1"
                     /translation="MGTAAIAVGAFLASRPSFLEDLYSSRLILIFVIFVYLLLLGMVA
                     TIEFPYSYVVDYPGLILYLLFVLGLLKMKAKAFLWFIAIVLPVSAFALYLGDIASVDQ
                     AEAFSQKATHEALPVFASYYYVSSMIYLTSSMIVGYAICCHLERDARAAFHRERELDI
                     FNQTLVETRRDIENKTLALIAAKEELRTSAERANLEKSKFLADAVHDLSQPTQAVSLL
                     AESARLALARNDLRTASNLIELTGRAAKTARSSFQAVLEISRLESGLIKPNLTAFGVQ
                     DLVEEALAPLRIIAQAKGVKVRVRMPRDVRSMTLSDRDLLLRAVANLASNAIKYSDQE
                     KGERQCVLIGVVPLPNRVRIDVIDNGIGIPESKRSEVFKPFVQLRNPEQNREKGLGLG
                     LSIVQAIIAVLPEHRLDMRSAEGRGTRFSLELPRYVGTPISPAEVSGSIAGTADLASL
                     FV"
     gene            1503..2222
                     /locus_tag="VNR65_00360"
     CDS             1503..2222
                     /locus_tag="VNR65_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17155.1"
                     /translation="MLHQKGALDLGCPERRGREQRLSYCREAADHRAISAPDFLASWR
                     AVQIMVWHPVHSDVTPSATPATFRGVSLLFSAHLAAGCDKATFVSTRRREAPALRWSD
                     SLATTTRIPLPPATIARASGNQHFLLGQPSKKIGMAPDEVRDRYPKRQSPSKQFSKTS
                     GLEMTRNRFEELPDFRDDGRAWTLLNSDQPNPEEGHRLVKALLAIKDAQVRRALIEFA
                     EALAREDRQRLWCTDRAVAVF"
BASE COUNT          542 a          681 c          629 g          462 t
ORIGIN      
        1 caaacgaaca gagaagcaag gtccgccgtg cccgcgattg agccgctgac ctcagccggc
       61 gagatgggcg ttcccacata acgtgggagt tcgagcgaga agcgggtccc acgcccctct
      121 gccgagcgca tgtcgagacg atgttctgga agaacggcaa tgatcgcctg cacaatcgag
      181 aggcctagac cgaggccttt ttcgcggttt tgctcgggat tgcgaagttg cacgaacggc
      241 ttgaacactt cgctccgttt gctctctggt atgccgatgc cgttgtcgat cacatcgatg
      301 cggaccctat tcggcagcgg aacgactccg atcagcacgc attggcgctc tcccttctcc
      361 tgatccgaat acttgatcgc gttgctcgcg agattggcca ccgcgcggag cagcaggtcg
      421 cggtcactta aggtcatcga gcgaacatcg cggggcatgc gcacgcgcac cttgacgccc
      481 ttggcctgag cgatgatgcg caatggcgct aaagcctcct ccacgagatc ctgcacccca
      541 aatgccgtca ggttcggttt aatcaggccc gattccaatc tggatatttc caaaaccgct
      601 tgaaacgagg aacgcgcggt cttggcggcc cgccctgtca actcgatcag attagacgcc
      661 gttctcagat cgttgcgggc cagcgcgagc cgggcggatt ctgccagaag gctaacggcc
      721 tgcgtcggtt ggctgagatc atggacagca tcggccagaa actttgactt ttccagattg
      781 gcgcgttccg ccgaggttct cagctcctct ttagccgcaa tgagggcgag ggtcttattc
      841 tcaatgtcgc gacgcgtttc cacaagcgtt tgattgaaaa tgtccagttc ccgctcgcga
      901 tggaatgcgg cgcgtgcgtc acgttcgagg tggcaacata ttgcatagcc aacgatcatc
      961 gatgacgtaa gatatatcat agatgatacg tagtaataac tcgcgaaaac cggcaacgct
     1021 tcgtgcgtcg ccttttgcga aaacgcttct gcctgatcaa cagacgcaat atcccctaag
     1081 tacaaagcga acgcgctaac tggcaaaaca atcgcaataa accaaaggaa agcttttgcc
     1141 ttcattttta gcagtcccaa cacaaacagc aagtataata tcaaccccgg ataatcaacc
     1201 acgtagctgt atggaaattc gattgttgca accatcccga gcaacaacag gtatacgaag
     1261 atcacaaaaa tcaagattaa tcggcttgaa tacaggtctt ccaaaaaact cggtcgggac
     1321 gccaaaaaag cacctaccgc gatggctgcc gtgcccaaga cccggagaac gagaactttt
     1381 gggaaaagag cgtcggcccc ggaacctggg ccaacattca tataatcgac cgacgaatag
     1441 gttacccaga agagtagcgc gagccagatt ccgatgcgcc gtggggggcc actcaggcga
     1501 gcatgctcca tcagaaaggc gcgctcgacc tcggctgtcc cgaacgacgc gggcgcgagc
     1561 agcgcctgag ctactgccgc gaagccgctg atcaccgagc gataagcgct cctgatttcc
     1621 tcgcgtcctg gcgcgccgtg cagatcatgg tctggcaccc cgtccactcc gacgttacgc
     1681 cgagcgctac acctgccact tttcggggcg tttcgctact tttctccgcg cacctcgccg
     1741 ccggctgtga caaagcgacg ttcgtctcga ccagaagacg cgaggcgcca gctttacgct
     1801 ggtctgatag tctcgctacg acgacgagaa ttcccctgcc tccagcgacc attgcgaggg
     1861 cttcaggtaa tcaacacttc cttctgggtc agcccagtaa aaagataggg atggcgcctg
     1921 acgaagttcg ggatcgatat cccaagcgcc agagcccatc taagcaattc agtaagacct
     1981 caggcttgga aatgacccgc aacagattcg aagaactccc cgacttccgc gacgacggca
     2041 gagcttggac gctcttgaat tcagaccaac cgaacccgga ggaagggcat cgccttgtaa
     2101 aggctctttt ggcgataaag gacgcccaag tgcgccgggc gctcatcgaa ttcgccgagg
     2161 ccctcgcgcg ggaggatcgg cagcgacttt ggtgcacgga tagggcggtg gcggtctttt
     2221 aaggagttta gggacagatg atcgacgctg ccatctcgag gattgatcgt gccgttcaaa
     2281 ttccattcca agggccgacg ccacattccc cgtc
//
LOCUS       DATMGX010000022         2821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_407812, whole genome shotgun sequence.
ACCESSION   DATMGX010000022 DATMGX010000000
VERSION     DATMGX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407812"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1948)
                     /locus_tag="VNR65_00365"
     CDS             complement(<1..1948)
                     /locus_tag="VNR65_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HWJ17156.1"
                     /translation="MPQTLSAISHLSPRPAGSAAVKGKSQPLKDDKTFASLLGFEKPA
                     KAASGSKKLRSGIDSSQAKTNDDVADFSQSGSNERASSQADASLACTAVMANQTQFIP
                     AVSLASYIPISGTKEDVSLTQNSQSADASAALQTSRPLPQSSPKGLLSGTGATATAAQ
                     DKNDAVQSYETSGDKQASRAPTVSEQTAAAAAVSSPATDTAATSLKTSLASAASALDG
                     VPNKAKPAFALAAADSSSALDTATTNLNASFAPYAPAMDRVPKDTAPTLAPRFAAAPD
                     TTAKTSEAQSSPNLPRTATAAPQSDRITSDANSDGLRISVSDARSGITSKIAIPPSSA
                     DPVKAPDAAVVVRSQTHFVPEPAKAGVGIAGQTASSAQDEGAAAGQTTLPPSGKQRDS
                     ELSTSISSPRNDVAPKGATPALAPDQADPSQTAAFAASQTKAGASSAEQWSNMTADGS
                     SFADTQAENPANAVSASRLNVTPNPGGGGNSDGGSAKDKNSHNHPGDGPSASADVPRS
                     SEVSLAIPLGASGASPSTPAQQIINEIQRAIPAADKSQALTSALQPTLDGQQPLKTIT
                     VALSPASLGTVAVELSLKSGQLGVKLRVQEADTVQLLRQDGSLEKLLESAGYAVQSLS
                     IHLSPQPSQPPQAQGQATANGQSFS"
     gene            complement(1948..>2821)
                     /locus_tag="VNR65_00370"
     CDS             complement(1948..>2821)
                     /locus_tag="VNR65_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17157.1"
                     /translation="EANTNEKKLFDGIMASGRGRTAEAESILLSLDATSFGAMRGGHL
                     SLTQALLTSRVNPERAYELFEKARLLLPGTLVEEAALRQIVVLAAKTSNRERFTKAAI
                     SYLSRFRRSAYVAGFETQLAFHIVRFEGRDGLLILQELLNAQPQGWDRCVACFLASIA
                     EQAILAGKIDLARTAAQAAMPFATDNSAEKQRVLLYKGAALIVTNDFARGLDALNSVR
                     TSDLNPKDKELHLASLSLAAKLRATPMLLTQAKLDASASGVPKHGHNFLPGERQAEAK
                     EALASVDAILAQAR"
BASE COUNT          529 a          773 c          892 g          627 t
ORIGIN      
        1 tggagaaact ttgcccattt gctgtcgcct gtccctgcgc ctggggcggt tgactgggct
       61 gcggcgagag atggatggat aggctttgga ccgcgtaacc tgccgattcc aagagctttt
      121 cgagagaacc atcctggcga agcaattgga ccgtgtccgc ctcctgaact cgcagcttta
      181 caccgagttg tccgctcttg agcgaaagct ccacggcaac agtcccgagg cttgcgggag
      241 agagcgcgac tgtaatcgtc ttcaggggct gctgaccatc gagcgtaggc tgcaacgcac
      301 tcgtaagggc ttggctttta tcggccgctg ggatagcacg ctgaatttca ttgataattt
      361 gttgtgccgg tgtcgagggt gacgcgccgc tcgccccgag ggggattgca aggctaacct
      421 cggacgaccg cggcacatcg gcagatgctg acggtccgtc gccaggatga ttatgagagt
      481 ttttgtcctt agccgatccg ccatcagaat tgccgccgcc gccaggattt ggcgttacgt
      541 tcagtcgaga ggcagaaacg gcatttgccg gattctcagc ctgcgtatcc gcgaagcttg
      601 atccgtctgc ggtcatgtta gaccattgtt ccgccgaaga ggctcctgcc tttgtctggg
      661 acgccgcgaa ggctgcggtc tgcgaaggat cggcctggtc aggcgctaac gctggtgtcg
      721 cacctttggg cgcgacgtca tttcggggag aggagatcga agttgaaagc tcggagtcgc
      781 gttgtttccc cgaaggcggg agggtagttt gccccgccgc ggctccttca tcttgcgctg
      841 aactcgccgt ctgcccggca atgccaaccc cggccttcgc aggttcgggg acaaaatgcg
      901 tttgcgagcg cacgacaaca gctgcatctg gtgctttgac gggatcagcc gatgaaggag
      961 ggatcgcgat cttggaggtg atgccgctcc gtgcatctga gacggagatt ctcaaaccat
     1021 ccgaattggc atcggaagtg attctgtcgc tttgaggtgc cgcggtcgcc gtgcgcggga
     1081 gattgggaga agactgtgct tctgaagttt ttgccgtcgt atccggggcg gcagcgaacc
     1141 tgggggcgag agtaggcgcg gtgtctttcg gcaccctatc catagccggg gcataaggag
     1201 cgaaggacgc attcaagttt gtcgtagccg tatccaaagc cgatgaagag tctgcggcag
     1261 caagggcgaa ggcaggcttg gctttgttcg gcaccccatc cagagccgag gcagcagaag
     1321 cgagggacgt tttcaagctt gtcgctgccg tatccgtggc cggtgaggag actgccgcag
     1381 ctgcggctgt ttgctcgctg acggtcggag cgcggctcgc ctgtttgtcg ccagaggttt
     1441 cataggactg cacggcgtca tttttatctt gtgccgccgt tgcagttgct ccggtgccgc
     1501 tgagcaaccc tttcggcgag gattgcggca aaggccttga tgtctgcaac gccgcgctcg
     1561 cgtcagccga ttgagagttt tgggttagcg aaacatcctc tttcgtgccg gatattggga
     1621 tataggaggc taaagagact gccgggatga actgggtctg attggccatg actgcagtgc
     1681 aggccaagct tgcgtcggct tggctactgg ctcgctcatt gctgccactt tgcgagaagt
     1741 ctgcaacgtc gtcgttagtt ttggcttgcg atgagtcgat gcctgagcgg agtttcttcg
     1801 aaccggacgc ggctttggcg ggtttctcga agccgagcag gcttgcgaat gtcttgtcgt
     1861 ccttcaacgg ctgagacttt cccttcacag ccgcgctgcc ggccggtctt ggcgaaagat
     1921 gggatatggc cgagagtgtc tgaggcatca ccttgcctgc gctaatattg catccacgct
     1981 tgccaacgcc tccttcgctt ccgcctgccg ctcgccagga aggaaattat gaccatgctt
     2041 cggcacacct gaagcggaag catccaactt ggcctgagtt aacagcatgg gggttgcccg
     2101 aagcttggca gcaagcgaga gcgaggcgag atgcaactcc ttgtctttcg gattgagatc
     2161 ggatgtccgg acggagttca gggcatccag gccgcgggcg aagtcgttgg tgacgatgag
     2221 agcggctccc ttgtatagaa gcacgcgctg cttttcagct gagttgtccg tagcaaaggg
     2281 catcgctgcc tgtgccgcgg tccgggccag atcaattttg cctgccagga tggcttgttc
     2341 ggcgatgctc gccaggaaac aggccacgca ccggtcccag ccttgcggct gagcattcag
     2401 aagctcctgc aaaatgagca gcccgtcccg tccttcaaat cgaacgatat gaaacgcaag
     2461 ctgggtctca aagcccgcca cataggctga ccggcgaaag cggctgagat aggagatagc
     2521 ggctttggtg aacctttccc tgttgcttgt cttcgcggcc agaacgacga tctggcgcaa
     2581 ggcggcctcc tcgaccaacg tacctggtaa aaggaggcga gccttctcga aaagttcata
     2641 ggcacgctcg gggtttaccc gggatgtcag caaagcttgc gtaaggctta gatgccctcc
     2701 gcgcatggcg ccgaagctgg tggcgtccaa cgaaagcagg atcgactcgg cttccgccgt
     2761 gcggcccctg ccagaggcca tgattccatc gaacagcttc ttctcattcg tattggcctc
     2821 a
//
LOCUS       DATMGX010000023         2320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_24943, whole genome shotgun sequence.
ACCESSION   DATMGX010000023 DATMGX010000000
VERSION     DATMGX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24943"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="VNR65_00375"
     CDS             <1..97
                     /locus_tag="VNR65_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ17158.1"
                     /translation="RQFLLRGIEKVRAEWAMICMAHNLTKLAKAA"
     gene            complement(149..868)
                     /locus_tag="VNR65_00380"
     CDS             complement(149..868)
                     /locus_tag="VNR65_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWJ17159.1"
                     /translation="MSHRFKIAAPGLIWTRLLVFATAAIVTFYAANAPAHAEAMQEKQ
                     EIVILSFGDSLMAGYQIPIDKAFPAQLEAALREKGYSVRVVNSGVSGDTAAEGLARLD
                     WSLNEKVDGAIVEFGANDALRGIEPKITEPSLDEILKTLKERRIELLVAGMEAPRNWG
                     KDYNDAFRGMYPRLAAKYGALLYPFFLKDVATVRSLNLADGLHPTPEGVALVVRNILP
                     EVEKLIARIREKREMKRVELR"
     gene            935..1615
                     /locus_tag="VNR65_00385"
     CDS             935..1615
                     /locus_tag="VNR65_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ17160.1"
                     /translation="MDLKAVTLTLKSKAGPVEILRGIDLTVPEGQSVALIGPSGSGKS
                     SLLMVMTGLESATSGQVLIAGQDFTRMSEDQLAEVRGRSIGIVFQSFHLIPTMTAIEN
                     VALPLEFLNRRDAFERAEVALADVGLSARHHHVPGELSGGEQQRVALARALAAQPKIL
                     FADEPTGNLDGETGAQIMELIFTLHRRIGTTLILITHDAALARRCERIVAMKDGRIAH
                     QVHETAYA"
     gene            1612..>2320
                     /locus_tag="VNR65_00390"
     CDS             1612..>2320
                     /locus_tag="VNR65_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17161.1"
                     /translation="MTAAIESRTEKRGARFQLPLALRLALRDLRGGFAGFTIFLICIA
                     LGTGSIGAINSLSDAIQDSLAREGTVLLGGDVEATLLHRQASPEELAYLKSFGEVSEV
                     ATMRAMARRPDGSAQALVDLKAVDDAYPLYGSVTFEEGGGLAAVRQGGLAVDRSILDQ
                     LQVSPGDSISIGNSLLPVKAVIEQEPDRLAAGPAFGARILLSPDTLKKTGLVEPGSLV
                     RWAYRIKTATGVPPEFRK"
BASE COUNT          437 a          724 c          699 g          460 t
ORIGIN      
        1 ccgccagttt ctgctgcgcg gtatagagaa agtgcgtgcc gaatgggcca tgatctgcat
       61 ggcccataac ctcacaaagc tcgcaaaggc tgcatgagag gtctttgctg ccgccgttac
      121 caggccggac gcctatctgg acgggctcct agcgcaattc aacccgtttc atttcacgct
      181 tctcgcgaat gcgtgcgatg agtttctcga cctcggggag gatgttgcgc acaaccaggg
      241 cgacgccctc gggcgtggga tggagcccgt ctgccagatt gagggaacgg acggtcgcaa
      301 catccttcaa aaagaaggga tagagcagcg cgccatattt tgccgccagc cgcggataca
      361 tcccgcggaa agcgtcgtta taatcctttc cccaatttcg aggcgcttcc atgccggcga
      421 caagcaactc gatgcgcctt tccttcagcg ttttcaggat ctcatcaagc gatggttccg
      481 tgatcttggg ctcgatgccg cgcaaagcgt cgttcgcgcc gaactccaca atcgcgccgt
      541 cgaccttctc gttcaggctc cagtcgagcc gcgccagccc ctccgcagcc gtgtcgccgg
      601 aaacgcctga attcacgacg cgcacggaat agcctttctc cctgagcgcc gcctccaact
      661 gtgccggaaa tgctttgtcg atcgggattt ggtagccggc catcaagctg tcgccgaacg
      721 agagaatcac gatctcttgt ttctcctgca tcgcttccgc gtgggccggc gcgttcgcag
      781 catagaatgt tacaatcgcg gccgtcgcaa aaacgagaag ccgagtccat atcaggccgg
      841 gcgcagcaat cttgaaacgg tgactcattt gcaggttccc tcagtttccg taagccacgc
      901 accggggatc gcagccggac gggcttcccc ggtcatcgat ctaaaggccg tgacgctgac
      961 actcaagagc aaggctgggc ctgtcgagat tttgcgcggc atcgacctta cggtgccgga
     1021 agggcaatcg gttgcgctga tcgggccgag cggctccggc aaatcatcgc tcctgatggt
     1081 gatgacgggg ctcgagtccg cgacttcggg acaggtgctc atcgcgggcc aggacttcac
     1141 gcgaatgagc gaagaccaac tggcggaggt gagaggccgc tccatcggca tcgtatttca
     1201 gtccttccat ctgatcccga ccatgaccgc gatcgagaat gtggcgctgc ctctcgagtt
     1261 cctcaaccgc cgggatgcct tcgaacgcgc cgaggtcgct cttgccgatg tcggtctttc
     1321 cgcccgtcat catcacgttc cgggcgaact ttcaggaggc gagcagcagc gcgtcgcgct
     1381 ggcacgcgcg ctcgcggccc aacccaaaat cctgtttgcc gacgaaccga ccggaaatct
     1441 cgatggcgaa accggcgcgc agatcatgga gcttattttc acgctgcatc gcaggatcgg
     1501 aacgactctc attcttatta cgcacgacgc cgcactcgcg cggcgctgcg agcgcatcgt
     1561 ggccatgaag gacgggcgca tcgcgcacca ggtccatgag acagcctacg catgacggcc
     1621 gcaattgagt cgaggaccga aaagcggggc gcccggtttc aactgccgct cgcgcttcgc
     1681 cttgcgctgc gagatttgcg cggcggcttt gcgggcttca cgatttttct gatctgcatc
     1741 gctcttggga cgggatccat cggcgctatc aacagccttt cggatgcaat ccaggattcg
     1801 cttgctcgcg agggaacggt gctccttggc ggcgatgtcg aggcgaccct tttgcatcgc
     1861 caggcgtccc cggaagagtt ggcgtatctt aaatcgttcg gtgaggtgag cgaggtcgcc
     1921 accatgcgcg caatggcgcg caggccggac ggcagcgcgc aggcgctcgt cgatttgaag
     1981 gctgtcgatg acgcctatcc gctttatggg tctgtcacgt tcgaggaggg tggcggcctt
     2041 gctgcggtga ggcaaggcgg cctcgccgtg gaccgcagca ttctcgatca actccaggtg
     2101 agccctggcg acagcatttc gatcggcaat agcctgcttc cggtcaaagc tgtcatcgag
     2161 caagagccgg accgcctcgc cgcggggcca gctttcgggg cgcgcatcct gctctcgccc
     2221 gacaccctga aaaagaccgg tcttgtggag ccgggaagcc tcgtccgctg ggcctatcgc
     2281 atcaaaacgg cgaccggcgt cccgcccgaa ttcagaaaag
//
LOCUS       DATMGX010000024         4564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_24885, whole genome shotgun sequence.
ACCESSION   DATMGX010000024 DATMGX010000000
VERSION     DATMGX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24885"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VNR65_00395"
     CDS             <1..365
                     /locus_tag="VNR65_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17162.1"
                     /translation="FHTIASAHAFALLPLGLGRWMLAAPFTSQLPLLLWLSVRCGPMF
                     AATGSLVLGLSILGSFTLEHGRFADPNFSLDERLAATDFAILATAFVALAVAALIAER
                     RDAELAARPRRSTAQTQP"
     gene            322..2484
                     /locus_tag="VNR65_00400"
     CDS             322..2484
                     /locus_tag="VNR65_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWJ17163.1"
                     /translation="MPQGQGGARLKLSLEAGKLGLWELDPKTGAFDATKRARTCFGLA
                     ENASLSLGAITDALHPGDRELWQSQLQRVCEEGAALDVECRTRATDGSMRWLHLTGQM
                     VGRRMPDARLLIAGAVLDITEQKQIASLKENAERLRWFVEQAPVAIAMFDRNMRYMAV
                     SRLWRTDYALGRPASLAAPITSSSPEITPFWKDVHQRGLAGETLRDERDPFKHADGRV
                     QWLSWEVRPWHDAGGAVGGIFIVSEDITPKVKAERALSESREDLNRAQAVAHTGSWRL
                     NVRTNELSWSDETFRMFGVPPATNLTYESFLDVVHPIDRERVDRSWKAALEGAPYDIE
                     CRVIADGITKWIHARSELGLDFAGNVLGGFGTVQDITYKKHAEEALRQSEERLRAIVD
                     TAVDAIIIIDENGLIVSINPAAERMFGYAASDIVGENVSILMTGPHRTAHDAYISAYR
                     RTGKAKIIGIGREVEGRRKDGSAFAVDLAIAEWQVAGKRYFTGIIRDISERKRQEQKV
                     ALLLREVNHRAKNMLALVQAIASQTAVPQGSEFVERFSERLAALAASQDLLVMSGWQG
                     VNARKLVRSQLSHFEELIDDRIKLAGPPLNLSAAGAQAIGMALHELATNAGKYGALSN
                     STGRIEIAWRLDRKAHSDQFTLSWVEAGGPPVFPPKQSGFGTTVIEAVPRMELDAEVT
                     LVYAPEGLCWRLECPAERVLEIPSGFEALREASAERKL"
     gene            2481..2834
                     /locus_tag="VNR65_00405"
     CDS             2481..2834
                     /locus_tag="VNR65_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17164.1"
                     /translation="MKARILVVEDNALVAFELCRALEAAGFEVAGPAPSVAQALTLIK
                     ERSCNAAVLDIHLRGETSEAVAHVLAALAIPFMTMTGYARTQQPPIFEGVPHFTKPVP
                     SSMVVAELRKLAPEA"
     gene            complement(2902..4074)
                     /locus_tag="VNR65_00410"
     CDS             complement(2902..4074)
                     /locus_tag="VNR65_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17165.1"
                     /translation="MNSPLIWRRSAILFFSVILFSQPTFAGDTALNDNARFLAGMDVV
                     AGSALEPLAKDKSFVRHSRFLNRAWAVLDSTQLSRVKAWSTENLKKTQPSLFYMFSGP
                     DFLYANAFFPKAATYVMAGLEAPGDIPDLTKLSRSSVPKELSALRASLRSVFSYSFFI
                     TKQMKKDLYGRRLTGTVPVLFVFLAHSGKTIESVDYVYLDEEGKLNPADATAQAREKY
                     DTVGVKITFSGDDKLIQTLYYFKTDLSNQGTEHSGFLKFCAQVGGGDSLIKSASYLLH
                     GEEFSHVRDFLLEHSSTIVQDDSGIPLKNFGMKDWNLEPFGSYVRPISLFHHNYQPDL
                     HGFFVKRHPHPLRFSFGYQWRSGKSMVLLAMKEVTRSYAQQKALVQPAKLDEVTRK"
     gene            complement(4252..>4564)
                     /locus_tag="VNR65_00415"
     CDS             complement(4252..>4564)
                     /locus_tag="VNR65_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17166.1"
                     /translation="ETALPLETCIGPQLAATLQNKIPGLKPLQRCQITKVFYTGDEGG
                     IVCSLDLPGWAGKEVYLASITHLVFDPRLPLARRIAIYQKHRAKCIRREAAAGQPTAF
                     R"
BASE COUNT          986 a         1258 c         1334 g          986 t
ORIGIN      
        1 cgttccatac gatcgcctcg gcccatgcct tcgcccttct tccgttgggg cttggccgct
       61 ggatgctggc ggccccattc acgagccagc tgccgctgct tctttggctt tccgttcgct
      121 gcggcccgat gttcgcggcc acaggatcct tggtgcttgg gctttccatc ctgggttcgt
      181 tcacgctcga gcatggccgc ttcgccgacc ccaatttttc tttagacgag cggcttgccg
      241 ccacggattt cgccatcctc gccacggcct ttgtggctct cgcggttgcc gctcttatcg
      301 ccgagcgcag ggacgcggaa cttgccgcaa ggccaaggcg gagcacggct caaactcagc
      361 cttgaggctg ggaagcttgg cttatgggaa ctcgatccga aaactggtgc gttcgatgcc
      421 acgaaacggg cgaggacctg tttcggcctg gcggagaacg cttctctctc acttggagcc
      481 ataaccgacg cgttgcatcc gggcgaccgg gagctttggc aaagccagct ccagcgtgtt
      541 tgtgaggagg gagcggcact cgacgtggag tgcagaacgc gagctacgga tggaagcatg
      601 cgctggctgc atctcacggg tcaaatggtc ggtcgccgca tgcccgatgc acggctcttg
      661 atcgccggag ccgtgctgga catcacagag cagaaacaga tagcgtcgtt gaaagagaac
      721 gccgagcgtt tgcgctggtt tgtggagcag gctccggtcg cgatcgcaat gttcgatcgt
      781 aatatgcgct atatggctgt cagccgcttg tggcggaccg actatgcatt ggggaggcca
      841 gcatcattgg ccgctcccat tacgagctct tccccggaaa tcacgccgtt ctggaaagac
      901 gtgcatcaac gcggcctcgc gggcgagact cttcgggatg agcgggaccc atttaagcac
      961 gccgatggca gagttcagtg gctgagctgg gaagtgcggc cctggcatga tgcgggcggc
     1021 gccgtgggcg gcatcttcat tgtcagcgag gatatcacgc ccaaggtcaa ggccgagcgg
     1081 gcgctcagcg aaagccgcga agacctgaac cgggcccagg ccgtggctca caccggcagc
     1141 tggcggctta atgtcaggac gaatgagctc tcctggtcgg acgaaacatt tcgcatgttt
     1201 ggtgtcccgc cggccacgaa cttgacctat gagagcttcc tcgatgttgt ccatcccatc
     1261 gaccgcgagc gtgttgaccg gagttggaag gccgctctcg aaggagcgcc gtacgatatt
     1321 gagtgtcgcg tcattgccga cgggatcacg aagtggattc acgcccgctc ggagctcgga
     1381 ttagatttcg ccggtaatgt tctcggcggc tttggcaccg tgcaagacat cacctacaaa
     1441 aagcacgctg aagaagcgct ccgtcaaagc gaagagcggt tgcgtgccat cgtggatacc
     1501 gccgtcgacg ccattatcat tatcgacgaa aatggcttga tcgtatccat caacccggcg
     1561 gccgaacgaa tgttcgggta cgcggctagc gatatcgtcg gtgaaaatgt ttccattttg
     1621 atgaccgggc cgcaccgcac cgcgcatgac gcttacatct ctgcctatcg acgcacgggc
     1681 aaagctaaaa tcatcggtat tggtcgggag gtagagggcc ggcgcaagga tggatcggca
     1741 ttcgcggtcg atctcgcgat tgcggaatgg caggttgcgg gaaagcgcta tttcaccggt
     1801 attatccgcg acatctccga gcgcaagcgg caggagcaaa aggttgcgct tcttctgcgt
     1861 gaggtcaatc acagggccaa gaacatgctt gcccttgtgc aagcgattgc atcgcagaca
     1921 gcggtgcccc agggcagcga attcgtggag cgtttttcgg agcgccttgc ggcgctggca
     1981 gccagtcagg atctccttgt catgagcggt tggcaggggg tcaacgctcg caagctggtg
     2041 cgctcgcaac tctcacattt tgaagagctg atcgatgaca ggatcaaact cgcaggacca
     2101 ccgttgaatt tgtcggcggc cggggcacaa gcgatcggga tggcgcttca cgagcttgca
     2161 accaatgctg gtaaatatgg agcgttgagc aattccacgg gcagaattga aatcgcctgg
     2221 cggctcgaca ggaaggcgca cagcgaccaa ttcactttaa gttgggtcga agcgggggga
     2281 ccgcctgtgt tcccgccgaa gcagtccggg ttcggaacga ccgttattga ggccgtgcct
     2341 cgcatggagt tggacgccga ggtcactctc gtttacgcac cggaaggatt atgctggcgt
     2401 cttgagtgtc ctgctgagag agttctggaa attccatctg gtttcgaggc cttgcgcgag
     2461 gcctcagcag agagaaagct atgaaagcac gaattttggt ggtcgaggac aacgcgcttg
     2521 tggcctttga gctttgccgg gctctcgaag cggcgggttt tgaggtcgcg ggacccgccc
     2581 cttcggtggc gcaggccctc acgctcatca aggagcgcag ctgcaatgcc gccgtgcttg
     2641 acatccattt gcgcggggaa acttcggagg ctgtcgccca tgtgctcgcg gcgctcgcca
     2701 ttccgttcat gaccatgacg ggctacgcgc ggacccagca acctcccatc ttcgagggtg
     2761 ttccccattt cacgaagccg gtcccatcat ccatggtggt ggccgaactt cgcaaactgg
     2821 ctcctgaagc ttgatccctg tgcgaccgcc gtctgatgct gacgactata cgcgcatcaa
     2881 ttgaggctcg tttcgcggct cctattttct cgtaacttca tccagcttgg ccggttgaac
     2941 cagcgctttt tgctgggcat acgagcgcgt tacttctttc atcgccaaaa gaaccatcga
     3001 tttgccgctt cgccactggt agccaaagct aaaacgaagt ggatgaggat gccgcttcac
     3061 gaaaaatcca tgcaaatcag gctgataatt gtgatgaaat agggatatgg gcctcacata
     3121 gctgccaaat ggctctagat tccagtcctt catgccgaaa ttcttcaggg gaatgccaga
     3181 gtcgtcctgt acgatcgtgg agctgtgttc gagaagaaaa tcgcgaacgt gggagaactc
     3241 ctcgccgtgg aggagatagg aggcgctctt gatgaggcta tcaccaccac cgacctgcgc
     3301 acagaatttc agaaaaccgc tatgctccgt cccctgattt gagaggtcag ttttgaagta
     3361 gtagagcgtc tgtatcagct tatcatcgcc tgagaaggta attttgacac caaccgtgtc
     3421 gtacttttcc cgcgcttgag cagtcgcgtc ggccgggttc aatttcccct cctcatcgag
     3481 gtatacgtag tcgacgctct cgatggtctt gccggaatgc gctagaaaaa caaaaagaac
     3541 tgggaccgtg ccagtcaagc gccgcccgta tagatccttt ttcatctgtt ttgtaatgaa
     3601 gaagctgtat gagaaaacgc tcctaagaga agcgcgcaac gcagacaact ccttcggaac
     3661 ggatgatctt gatagctttg tcagatccgg aatatcaccc ggagcctcaa gaccagccat
     3721 tacataggtt gcggccttcg gaaaaaatgc gttggcatac agaaagtccg gaccgctaaa
     3781 catataaaag agcgacggct gcgtcttttt tagattttcc gtggaccacg ccttcaccct
     3841 tgaaagctgg gtcgaatcaa gaactgccca ggctcgattc aagaaacgag aatgccggac
     3901 gaagctctta tccttcgcta gcggctccaa agcgctacct gcgacaacat ccatgcctgc
     3961 gaggaagcga gcattgtcgt taagggccgt gtcgcccgcg aaggttggtt gagagaacaa
     4021 gataacagag aaaaagagta ttgcagatct tcgccagata agagggctgt tcatcgaggg
     4081 tcgattccaa ttacgatttg ggcttatgcg cacagcagta aagttgcccg caatgcatga
     4141 gaattctgtt aatcataaaa aggattatcg taagcgcccg cgacccacgc tgagttcaga
     4201 gaagacgagt ggatacagat ccaatgcccg ggatcgagcc taccgcagac gtcagcggaa
     4261 cgccgtgggc tgaccggctg ccgcctcgcg ccgaatacat tttgcgcgat gcttttggta
     4321 gatagctatc cgccgcgcca aaggcagtct gggatcaaaa acaagatgtg tgatcgaagc
     4381 cagatacacc tccttgccgg cccatcccgg tagatccaag ctgcaaacga taccgccctc
     4441 gtcaccagta tagaacacct tagtgatttg gcaccgctga agcggcttaa gacccggtat
     4501 tttgttctgc aacgtggccg ccaattgcgg accgatacaa gtttcgagcg gcagtgcagt
     4561 ctcc
//
LOCUS       DATMGX010000025         2588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_407365, whole genome shotgun sequence.
ACCESSION   DATMGX010000025 DATMGX010000000
VERSION     DATMGX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407365"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VNR65_00420"
     CDS             complement(<1..767)
                     /locus_tag="VNR65_00420"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HWJ17167.1"
                     /translation="MRVDSERIPDIGPSSFWDKLSPGRLFSRSDTIPVLRLSGTIGAA
                     TPLRQGLTIAGCATALERAFSIKNAPAVVLQINSPGGSPVQSRLIYERIRLLAWQKKK
                     KVYAFAEDVAASGGYMLACAADEIYADGSSLVGSIGVLSSGFGFVDLIHKLGVERRVY
                     TSGENKFQLDPFKPEKPEEIKRLKHIQEIVHQDFISLVKESRGDRIAAAGDSLFTGEF
                     WSGRQALELGLIDGIMDMRAKMRSVYGDDVRLKLISM"
     gene            complement(874..1602)
                     /locus_tag="VNR65_00425"
     CDS             complement(874..1602)
                     /locus_tag="VNR65_00425"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWJ17168.1"
                     /translation="MHLRQPRAGFRSGLDAVFLAAACPAQARDHVLEAGCGAGAASLC
                     LLARVAGAKVTGVEIDPGLTALAVENAAANDMTERFEGVAADVTAPRAALDAAGLRSD
                     AYDHVMANPPFFSHGRSRPAKDQGNARARAMPEGELEAWLRFLAASAKPSGTCTVIHT
                     AEALPELLSAFDRRFGDLRITPLYPKANAPAIRVIICGIKGSRAPVAITPGIVLHEQD
                     GTPTAAAEAILRDGCALFNTPHGK"
     gene            complement(1666..1917)
                     /locus_tag="VNR65_00430"
     CDS             complement(1666..1917)
                     /locus_tag="VNR65_00430"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HWJ17169.1"
                     /translation="MKELLRTTDIVLLTYVNSLLNDACVPVLIADIHVSSVEGSIGAF
                     PRRVLVAAEDWDESISVLTAAGLGDHLIDEAPPRGRASL"
     gene            2058..>2588
                     /locus_tag="VNR65_00435"
     CDS             2058..>2588
                     /locus_tag="VNR65_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWJ17170.1"
                     /translation="MNLAAQVQERGDPAAALEPLMRLVRDEMGEVNQIILSQARSDVA
                     LIPELARHLINSGGKRLRPMLTIGAAKLCGYTGEGHIKLAAAVEFMHTATLLHDDVVD
                     ESDMRRGKKSARMLWGNQASVLVGDFLLGQAFRMMVDVGSLGALKILSEASAIIAEGE
                     VMQLAASKNMSTTEEDY"
BASE COUNT          570 a          733 c          773 g          512 t
ORIGIN      
        1 tccatggaga tgagcttaag cctgacatcg tctccataaa ccgaacgcat cttcgctctc
       61 atatccataa tgccatcaat caggccaagc tcaagagcct gtcgaccgga ccagaactcg
      121 cccgtgaaca aactgtcccc cgctgcggca attctgtcac cacggctttc ctttaccagc
      181 gatatgaaat cttgatggac gatttcctga atgtgcttaa gccgtttgat ttcctctggt
      241 ttttccggtt tgaagggatc gagctgaaat ttattctcgc ctgacgtgta gacgcggcgc
      301 tcgacaccta acttgtggat aagatctacg aagccgaaac ccgacgacaa aacgccgatg
      361 gatccgacca gcgaagaccc atccgcgtag atttcatcgg ccgcgcaggc aagcatgtag
      421 ccacccgaag ccgcaacatc ttccgcgaaa gcgtacacct tctttttctt ctgccacgcc
      481 aggaggcgaa tgcgctcata aatgaggcgg gactgcactg gtgatccgcc cggcgaattg
      541 atttgaagga ccacggcggg cgcatttttg atactgaatg cgcgctcaag cgcggttgca
      601 catcccgcaa ttgtgagccc ctgcctgagc ggcgttgcgg cgccgatcgt gccggacaag
      661 cgcaaaacgg gaatggtatc gcttcgagaa aacagcctgc cagggctgag tttgtcccaa
      721 aacgagcttg ggcctatgtc tggaatacgc tcgctatcga ctcgcatgaa aaacccgcaa
      781 gactgagata atggagctga caaaggacag ccccgtcgct ttcctgggtc atgtgtgtac
      841 gggctggccg ttaacaagac atacccttaa tgttcatttt ccgtgaggtg tgttgaagag
      901 tgcacaccca tcccggagga tcgcttcggc ggcggcggta ggcgtcccgt cttgttcgtg
      961 caggacaatg ccgggcgtga tcgcaacggg cgcgcggctt ccctttatgc cgcatataat
     1021 aacgcggatc gcgggcgcat tcgcctttgg ataaagcggg gtgatcctca aatcgccgaa
     1081 acgcctgtcg aacgcggaaa gcagctcggg cagggcttcc gccgtgtgaa tgaccgtgca
     1141 ggttccggat ggcttggccg aggcggcgag gaagcgaagc caggcttcga gttcgccttc
     1201 gggcatggcc cgcgcccttg cgttgccttg atccttggct ggccttgaac ggccatgaga
     1261 gaaaaatggc ggattggcca tgacgtgatc gtaggcatcg gagcgaagcc cggcagcgtc
     1321 gagcgcagcg cgcggtgcgg taacatcggc ggcgacgcct tcgaagcgct cggtcatgtc
     1381 attggccgcc gcgttctcaa ctgcgagagc agtcaggccg ggatcgatct ccacgccggt
     1441 aactttcgcg cccgcaacac gggccagcag acacagagaa gcggctcctg ctccacagcc
     1501 agcttcaagc acatgatccc gcgcctgcgc cgggcatgcg gcggcaagga acacagcgtc
     1561 caagccggat cggaacccgg cccggggctg gcgcaggtgc acgcggccat ccaaaaaagc
     1621 gtcacacgta aagttgaaat gactggccat gctgaggccg ggatattaaa gtgacgctcg
     1681 gccgcggggt ggggcttcgt cgatcagatg atcgccgaga ccagctgccg tgaggacgga
     1741 tatgctctcg tcccaatcct cagcagcgac aaggacgcgc cggggaaaag cgccaatcga
     1801 tccttcaacc gagctgacgt gaatgtcggc gatgagcaca gggacgcatg catcgttcag
     1861 caaagagttg acataggtca gcagcacaat atcggtagtg cgaagcaact ctttcataga
     1921 acaagccttt tttgaacagg gccggcatca gggacattaa gccacgtggc taaattatca
     1981 aagcagtgtt tcactgggag gctgtggacg cgctcccagc tcgtcatttt gcccaatcac
     2041 caaattcgga ggatatcatg aacttggccg ctcaagttca ggagcgcggc gatccggctg
     2101 cggcgcttga gcctttgatg cggctcgtgc gcgatgagat gggtgaagtg aaccagatca
     2161 ttctctcgca ggcgcgttca gacgtcgctc tcattcccga gcttgcccga catcttatca
     2221 actccggcgg aaagcggttg cgcccgatgc tgacgatcgg agcggccaag ctgtgcggct
     2281 ataccggcga agggcatatc aagcttgcgg ctgccgtcga attcatgcac accgcaaccc
     2341 ttctccatga cgacgtcgtg gatgaaagcg acatgcggcg cggcaagaag agcgcccgca
     2401 tgttgtgggg caaccaggcc agcgtgcttg tgggcgattt ccttctgggc caggcgttcc
     2461 gcatgatggt cgatgtggga tcgctgggcg cgttgaagat cctgtcggaa gcttcagcga
     2521 tcatcgcgga aggcgaggtc atgcaactcg cggcgtcgaa aaatatgtcg acgacggagg
     2581 aagattat
//
LOCUS       DATMGX010000026         4218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_372180, whole genome shotgun sequence.
ACCESSION   DATMGX010000026 DATMGX010000000
VERSION     DATMGX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372180"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1578
                     /locus_tag="VNR65_00440"
     CDS             433..1578
                     /locus_tag="VNR65_00440"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ17171.1"
                     /translation="MQISKRTRVLRALSIPLLFCGVAVGLVTVQTAIQRQAERAGAAA
                     QQPQSMSAAHHTMMAGSMVSVADLSEAAGQGNLAARLEMGRRLAQGRGVEKNEGSAAA
                     YFRGIISEYENISAHDKRGPHVAAAFLEMARLYKAGVPEANVVANPAQAFSFLHHAAS
                     YFGDPIAQFELAKLLMSGDGVTKNTRAAAQWLLSASRKGYAPAQAQLGDLLWRGGDGV
                     RRVAGDGLGLLAIARRNAQGEDKVWVSTMFETARAEAQPIEILEANAFIVQELSSSHS
                     ALTSEVLINGESREGSGVAAAASALRASPADAAAPQAAAISGTSKALSELGASPMGFP
                     QNPFDPELTIKKQDGGSAAGILQMYKPQSPQGRVEAFTPTRYAGVSN"
     gene            complement(1591..>4218)
                     /gene="ligD"
                     /locus_tag="VNR65_00445"
     CDS             complement(1591..>4218)
                     /gene="ligD"
                     /locus_tag="VNR65_00445"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HWJ17172.1"
                     /translation="FVIQKHAARRLHYDLRLELDSVYKSWAVTKGPSLDPHIKRLAVH
                     VEDHPIDYGDFEGIIPKGEYGGGTVMIWDRGFWRPEGDAAKGYAKGHLAFELDGEKLH
                     GRWHLVRTKARPGDKKEQWLFFKSDDEFAHPEAEEDILEAAQDSVATHRTMDEIAGEQ
                     SAVWSSAGGLVRGDLSPSGDTSPNGTRGGRRSQKPALSRQKNGELGEHSSIKPSSIKG
                     AKKAAFPGFVEPCLALLVEKPPAGDGWLHEIKFDGYRLMAAIDGKSIRLLTRSGLDWT
                     DRFPGIAEAFADFPAKSALFDGEAVIEDENGVSSFSALQEALSERKTASAAVFFAFDL
                     LYLDGFSLHESALADRKDALAKLLSPNRHPSLRYSDHVVGSGQSMFDHACRLGLEGII
                     SKRRDAPYRSGRNGGWAKSKCINREEFVVAGYTPSTATRNAIGSLALGTYDAGKFIYA
                     GRTGTGFTQRSAQSLHKELQSSRIQRSPFANALSSEERRGLVFVEPRLVAEVEFRGWT
                     NDKRLRQAAFKGLREDKPASEVQLEMPRQDAGSANAQGQTIRPPNKKAGASIRPAKGG
                     DIEFSGIKLTHPDRLLWEGQGLTKLGLAEYYAEVAEYILPFIAGRPLALVRCPNGHAG
                     DCFYQKHSFAGLTDAVEIAHVEEKDGKAEEAIVIHDLRGLINLVQANVLEIHPWGARI
                     DDIDHPDTLIFDLDPGEGLEWTDVIEAAGDVRRRLRDLGIESFVKTTGGKGLHVVSHL
                     KPVADWDELKSFAHSIAIAMERDEPSKYISTMAKKARGGKIFVDYLRNGRGATAVAAY
                     STRARPGAPISTPVRWDELGPALTPARFTVENIGRRLAALKSDPWGGFFSRPQNIKEA
                     ISALEARPPVIRRTRSKSS"
BASE COUNT          831 a         1240 c         1260 g          887 t
ORIGIN      
        1 ctgtcttgga cctgtggaag ctgcataagt cgcgattgcc aagtcgccac tctaaagggg
       61 ctcgttgcaa caaaaatttc gagggttgtt ggctggggtc acgtcaccaa tgcggcgtcg
      121 cgcatagggg accagtttct attggcgcgc cgcgtggcct ggatgtcaca gtcactgatg
      181 tattcaagtc tgcagcgccg cgaacaggca gcgctaccgt aaccgttgtt tctgatggtg
      241 gtatggtccc atcccaagcg gagatgctcg ccttcaagtc gtcacaaagc gagcccgtcc
      301 gccttttgaa tgccaggacg gggtcaaaaa ttaactgcat gcctaaggca tttggtgact
      361 atgcgcaaca ggcgaggcca gcgtggcttc aagctgttac gcgtgttggg gctacggtgg
      421 aagtattgtg ccatgcagat ctctaagcga actcgtgtct tgagggcatt atccattccc
      481 ctcctgttct gcggagtggc tgtcggtctt gtcaccgtac agaccgcaat tcagcggcag
      541 gcagaaaggg caggtgcggc ggctcaacaa ccgcagtcaa tgtccgcggc gcaccacact
      601 atgatggccg gttcgatggt cagcgtggca gacctttctg aagccgccgg acagggaaac
      661 cttgcggcgc gtttagagat gggccgccgc ctcgctcaag gacgaggcgt ggaaaagaat
      721 gaaggcagtg cggcagccta cttccgcggt atcatcagcg aatacgaaaa catcagcgcg
      781 cacgacaagc gtggtccgca tgtcgctgcc gcgttcctag agatggcgcg gctctataaa
      841 gcgggcgtgc ccgaggccaa cgtcgtcgcc aaccccgccc aagccttcag cttccttcat
      901 cacgctgcca gctatttcgg agaccccata gctcaattcg aactcgcaaa gcttttgatg
      961 agcggcgatg gggtgacgaa gaatacgcgg gctgctgcgc aatggctcct gagcgcgagc
     1021 cgtaagggat acgcaccggc tcaggctcag ctcggcgatc tcctttggcg cggcggcgat
     1081 ggcgtgagac gcgttgcggg tgatggtttg gggttgcttg caatagcgcg ccggaatgcg
     1141 cagggtgagg acaaagtctg ggtaagcacg atgttcgaga cggcgcgcgc agaagcgcag
     1201 cccatcgaaa ttctcgaagc gaatgcgttc atcgttcagg aattgagctc ctctcattcg
     1261 gccctgacaa gcgaagtcct cattaatggg gaaagccggg aggggtctgg ggttgccgcg
     1321 gcggcaagcg ccttgagggc atccccggct gacgctgccg ccccgcaagc cgcagccata
     1381 tccggtactt cgaaagccct gtctgagttg ggcgcgagcc cgatgggctt tccgcagaat
     1441 cctttcgatc ccgaattgac gataaaaaag caggacggag gttcagcggc gggtattctc
     1501 cagatgtata agccgcagtc accgcagggg cgcgtcgagg ccttcacgcc gactcgatac
     1561 gcaggagttt ccaactgaca tttcaggatg tcaggaggac ttcgatcttg ttcgacgtat
     1621 cacgggcggc cttgcttcta aagcggaaat cgcttccttg atattttgcg ggcgcgagaa
     1681 aaagcccccc cacggatcgg atttcagggc tgccaatcgg cggccaatat tctcgacggt
     1741 gaaacgggct ggcgtgagtg cgggtccaag ctcgtcccat ctcaccggcg ttgatatcgg
     1801 cgcccctgga cgagcccttg tcgaataagc cgcgacggct gttgcgccac gaccgttgcg
     1861 caggtaatcg acaaagatct tcccgccgcg tgccttcttt gccatcgtgg atatatattt
     1921 cgatggttca tctctttcca tagcgatcgc aatgctgtgg gcgaaagact tcaactcgtc
     1981 ccaatccgcg acaggcttca gatgcgatac cacgtggagc cccttccctc ccgtcgtctt
     2041 cacgaagctc tcgatgccga ggtcccggag gcgtcggcgc acgtcacctg ccgcttcaat
     2101 aacatcggtc cattccaagc cctctcccgg gtccaggtcg aaaatcagag tatcgggatg
     2161 atcgatatcg tcaatgcgag cgccccaggg gtgaatttcc agcacattgg cctgcacgag
     2221 attgatgagg ccacgaaggt cgtggatgac gatggcttct tcagccttgc cgtccttctc
     2281 ctccacatga gcaatctcga ctgcgtctgt gagccctgcg aacgaatgct tctgataaaa
     2341 gcagtcgccg gcgtggccat ttgggcatcg caccagggca agcggcctcc cagcaataaa
     2401 gggaaggata tattcggcca cctcagcgta atattcggcg aggcccagtt tggtcagccc
     2461 ctgcccctcc caaagaaggc gatccggatg ggtcagcttg atgccggaga attcgatgtc
     2521 tccgcctttc gccggtctga tggacgcgcc cgctttcttg ttcggcggcc ggatcgtttg
     2581 accctgcgca tttgcactcc ctgcgtcctg gcgtggcatt tcgagttgca cctccgaggc
     2641 gggtttgtct tcgcgaagtc ccttgaatgc ggcctgacgc aatcgcttgt cgttcgtcca
     2701 gccacgaaat tcgacctccg cgacaagtct cggttcaacg aaaacgaggc cgcgccgttc
     2761 ttccgaagag agcgcatttg cgaatggcga tcgctggatt cgcgaggact gcagctcttt
     2821 atgaagcgat tgcgccgacc tctgcgtgaa ccccgttccc gtgcggcctg cgtaaatgaa
     2881 cttgccggcg tcgtaggtgc cgagcgccag cgatccaatc gcgtttcggg ttgcggtgga
     2941 gggggtgtaa cccgcgacca cgaactcctc gcgatttatg catttgcttt tcgcccatcc
     3001 gccgttgcgc cccgagcgat aaggcgcgtc gcggcgcttg ctgatgatgc cttcgaggcc
     3061 aaggcggcag gcgtgatcga acatggactg cccgcttccg acgacgtgat cggaatagcg
     3121 taaggaagga tgccggttcg ggctcaggag cttggcgaga gcatctttgc ggtcagccag
     3181 cgccgactcg tggaggctaa acccgtccag ataaagcagg tcgaaggcaa agaagacggc
     3241 ggcggaggct gttttgcgtt cggaaagcgc ttcctgcaaa gcggaaaagc tcgatacgcc
     3301 gttctcgtcc tcgataaccg cctcgccgtc aaatagtgca gatttcgccg ggaaatccgc
     3361 gaaggcctcc gcgatacctg ggaaacgatc ggtccaatcc aggccgctcc gggtgagaag
     3421 ccggatactt ttgccgtcga tcgccgccat gagccggtaa ccatcgaact tgatctcgtg
     3481 caaccagcca tcgccagcgg gtggcttctc caccagcaga gcgaggcacg gttccacaaa
     3541 tcctgggaag gcggccttct tagccccctt gatcgaggat ggttttatgg aggaatgctc
     3601 ccccaactca ccgtttttct ggcgggaaag agctggcttt tgcgagcgcc gacccccgcg
     3661 cgtaccattg ggggaagtgt cacctgaggg cgatagatcg ccccgaacca gcccccctgc
     3721 ggaagaccat acggcgcttt gttctcccgc gatctcatcc atcgtgcggt gtgtcgccac
     3781 cgaatcctga gccgcctcca ggatgtcttc ctccgcttcc gggtgcgcga actcgtcatc
     3841 cgatttgaag aaaagccact gctccttctt atcgcccggc cgcgccttgg tgcgaacgag
     3901 gtgccaacgg ccgtgaagct tttcgccgtc gagttcgaag gcaaggtggc ccttggcgta
     3961 gccctttgcc gcatcgcctt ccggtcgcca gaatccgcgg tcccagatca tgacggtgcc
     4021 cccgccatat tcccctttcg gaatgatccc ttcgaaatcg ccataatcga tggggtgatc
     4081 ctcgacatgg actgcaaggc gcttgatatg cggatcgagg ctcggacctt tggtgacagc
     4141 ccagctcttg tagacgctgt cgagttcgag acggaggtcg taatgaagcc ggcgcgctgc
     4201 gtgtttctga atgacgaa
//
LOCUS       DATMGX010000027         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_473353, whole genome shotgun sequence.
ACCESSION   DATMGX010000027 DATMGX010000000
VERSION     DATMGX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473353"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..336
                     /locus_tag="VNR65_00450"
     CDS             166..336
                     /locus_tag="VNR65_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17173.1"
                     /translation="MGESYWREAVNLNFLVDEGVVDREDRELFWDAETAEETWNGISR
                     WRDISGEPLQVA"
     gene            370..1758
                     /locus_tag="VNR65_00455"
     CDS             370..1758
                     /locus_tag="VNR65_00455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWJ17174.1"
                     /translation="MKLSFYGADRGVTGSCHMVECAGKRILIDCGMYQGSHELDEENS
                     QPFGFDPASIDYVLLTHAHLDHCGRLPLLAKRGFHGEIITTAASRELARLVMLDAAHL
                     QEEDARYPARIAARGSSAGHAVAPLYSVLDALNSFDHFGRMAAYDEPIEVADGVRATF
                     IDAGHILGSASIYLQLTESSQQSTVLFSGDLGKSGGPLLCDPAKPPAAASVVMETTYG
                     DRLHKPLGPSVEEFYDAVTDTFRRGGNVVVPTFALERAQELLYFLSQGIDHGRLTKST
                     QVFIDSPMAISATEIMRRHLDGLRPEIAKLIRDGNDPFHFPGLHFTRESAESVAINNI
                     HGGAIIMAGSGMCTGGRVRHHLQHNLGREDSSVVFVGYAAAGTLARHIIDGAKEVEIF
                     GERIPVRARIYTINGFSAHADQADLLAWQKQTGAKRTFLVHGEEESMRSFATHLGNTR
                     IDLPEPNQVFEL"
     gene            1917..>2129
                     /locus_tag="VNR65_00460"
     CDS             1917..>2129
                     /locus_tag="VNR65_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17175.1"
                     /translation="MTLAAAAVDSIPADLVVPRFLYYFVAVYFVASVALWLTHRFLLW
                     KKSNAKSKAPLDDPAWWLKPLSGDGAG"
BASE COUNT          427 a          599 c          630 g          473 t
ORIGIN      
        1 acgaaaggtt cgtcacggcg tgatggtcaa ccggccccgc acacaaaatt tcggatagtc
       61 ccaaggcgct ggtcgcattt cctgggggta tcgcacgtta gataacgtat tcgaagtgct
      121 cgcgctcatc caaacgcgca agatcaggcc tatcccggtc gtctggtggg cgagagctac
      181 tggcgcgagg ccgtcaatct gaatttcctg gtcgacgaag gcgtcgtaga tcgggaagat
      241 cgcgaattgt tctgggatgc cgaaacggcc gaggagacct ggaatggcat atcgcgttgg
      301 cgcgatatca gcggcgagcc cttgcaggtt gcatgagagg cggatcatcg catagctaag
      361 gagaatccct tgaaattatc attttacggc gccgatcgcg gcgtcaccgg ctcgtgtcac
      421 atggtcgagt gtgccggcaa gcggatcctg atcgactgtg gaatgtacca aggtagccat
      481 gaactggacg aggagaattc gcaaccgttc gggttcgatc ctgccagcat cgactatgtc
      541 ttgctgaccc atgcccatct tgatcattgt ggccgcctgc cgctgttggc caagagaggg
      601 tttcacggtg agatcatcac gacggcggcg tcgcgggagt tggcgcgctt ggtaatgctc
      661 gatgcagccc atttgcaaga agaagacgct cgttacccag cccgtatcgc cgcgcgaggc
      721 agttcagcgg gccacgccgt agcgccgctc tattcggtcc tcgatgcttt gaactccttc
      781 gaccacttcg gacggatggc ggcttacgat gagccgatcg aagtcgctga tggcgtgcgc
      841 gccaccttca tcgatgcggg tcacatcctt ggttcggcca gcatctatct gcagctgaca
      901 gaaagctcgc agcaatccac cgtgctattc tccggagacc ttggcaagtc tggcgggcct
      961 ttgctgtgcg atcccgcgaa gccgccagcg gcagccagcg tggtgatgga aacgacgtac
     1021 ggtgatcggt tgcacaagcc cctgggaccg tccgtcgagg agttctatga tgccgtgacg
     1081 gatacgttca ggcgcggtgg aaatgtcgtc gtccccactt tcgctttgga gcgagctcag
     1141 gaactgcttt atttcctcag ccagggaatc gaccatggtc gactgacaaa atcgacgcag
     1201 gtttttatcg actctcccat ggcgatctcc gccacggaaa tcatgcggcg tcatttggat
     1261 ggcttacggc cagaaatcgc gaagctcatt cgagacggaa acgatccgtt tcattttccg
     1321 ggattgcact tcacacgcga aagcgccgag tccgttgcga taaataatat ccatggcggg
     1381 gccatcatca tggccggctc gggtatgtgc actggcggtc gcgtgcgcca ccacctccag
     1441 cacaatctgg gtcgcgagga ttcgagtgtc gtgtttgtcg gctacgcggc cgctgggact
     1501 cttgcacgcc atattatcga cggcgccaag gaggtggaaa tttttggtga aagaatcccg
     1561 gtgcgagctc gcatttacac gatcaacggt ttctccgccc atgcggatca ggccgacctg
     1621 ttggcttggc aaaagcaaac cggtgcgaag cggacgttcc tcgttcacgg cgaagaagaa
     1681 tcgatgcgaa gcttcgcgac acatctcggc aacacccgaa tcgatctacc ggagccgaat
     1741 caggtgttcg agttgtaggc agcgtctgcc cgcacgtggg ctgtccctcc cgcgaactcg
     1801 gtccgacccg tgttagtatg ccaaagccga cggttcgcct attgtaggtt tgggcaaaca
     1861 aaggttttga cttgtcgctt ggcaagcgag cggcgtcttc agaaagcaga cctccaatga
     1921 ctcttgctgc cgccgccgtc gattcgatcc ccgcggactt ggtcgtgcct cgattcctat
     1981 attactttgt ggccgtatat ttcgttgcct ccgtggccct gtggttgacg catcgatttc
     2041 ttctctggaa aaagtccaac gctaagtcca aagcccctct agacgatccc gcttggtggc
     2101 tgaagcctct ttcgggcgac ggagcgggg
//
LOCUS       DATMGX010000028         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_406904, whole genome shotgun sequence.
ACCESSION   DATMGX010000028 DATMGX010000000
VERSION     DATMGX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406904"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VNR65_00465"
     CDS             complement(<1..652)
                     /locus_tag="VNR65_00465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWJ17176.1"
                     /translation="MKTHARVVVVGGGMMGASLLYHLALEGCTDTILVEKSELTSGST
                     WHAAGQCPSITGSYNLAKIHAYSNELYPRLEALTGQYVSWHPCGGLRLANNEHELAWL
                     KYVHGFSKIIGFSMDIVGLDEIRRLNPFLTTDGVLAAAWTRDDGHGDPSGLCNALAKA
                     ARDLGATVVRRNRVTDIRRRDSGEWEVFTEQGSIVAEMVVNAAGCYAREVAAMVGSD"
     gene            complement(914..1504)
                     /locus_tag="VNR65_00470"
     CDS             complement(914..1504)
                     /locus_tag="VNR65_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HWJ17177.1"
                     /translation="MAINPENLTKIQAAVVTSLHEHWGLFLSEGIFLLILGIAALLLP
                     AVASIAAAIVIGWVVLLSGVVGLISTLRMRSAPGFWWSLFSAVLAVIAGFILLGWPLS
                     GVVSLTLILAVFLLLEGIASIMLALDHRRRTSGRWGMLLASGIIDLILAAFIFLGLPG
                     TAVWILGLVVGVNMLFGGWALISMAWHARNAAPRTG"
     gene            1755..>2040
                     /locus_tag="VNR65_00475"
     CDS             1755..>2040
                     /locus_tag="VNR65_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWJ17178.1"
                     /translation="MARGGGSGFGLQDLPRISHGKGSYLYDEAGKRYIDGSGGPAVFR
                     VGHSHPEVNAAIARQMDAVAYGYRYLFTSSALEALSEMMLRISGNCFTDIV"
BASE COUNT          446 a          613 c          591 g          390 t
ORIGIN      
        1 catcgctgcc gaccatggcg gcgacctcgc gggcatagca accggccgca ttgacgacca
       61 tctcggcgac gatggagccc tgctcggtga acacctccca ctcgccgctg tcgcgccgac
      121 ggatgtcggt gacgcggtta cgccgcacca cggtggcacc caaatcgcgc gccgccttgg
      181 cgagcgcatt gcacaggccg gacggatcgc cgtgcccatc gtcccgggtc caggcagccg
      241 cgagcactcc gtccgtggtc agaaacggat tcagacggcg gatttcatcc aggcccacga
      301 tatccatgct gaagccgatg atcttcgaga agccgtggac gtacttcagc cacgccagtt
      361 cgtgctcatt gttcgccaac cgcaaaccgc cgcacgggtg ccagctcacg tactgtccgg
      421 tgagggcctc cagacgcggg tacaactcgt tgctgtaggc atggattttg gcgagattgt
      481 agctgccggt gatgctggga cattgcccgg cggcgtgcca ggtggaaccc gaggtgagct
      541 cgctcttttc gaccagaatc gtgtccgtac agccttccag ggccaggtgg tacagcaggc
      601 ttgcgcccat catcccgccg ccgacgacga cgacgcgtgc gtgtgttttc attgaagacc
      661 gatgagttgg ttatgggagg gcatttgcca caataaattg ctcctcatcg ccaggcaacc
      721 gggttgatac ctttgaacat agaattttcg cagcatagcc cgtttcgacc aatatcaggc
      781 cggtcgtcgg tttgccggca ttgacttggg ctgcgtgcct tgcatctttg ttatccgccc
      841 actgggcgag cgccgcccgc ttgccgatcg gtcggctggt tctcgccctc gaccacgagt
      901 tcgccgatga cagttaaccg gttcgcggcg cagcgttgcg cgcgtgccag gccatcgaaa
      961 tgagcgccca tccgccgaac aacatgttga cgcccaccac cagaccgaga atccacaccg
     1021 cggtgcccgg aagccccaga aatatgaagg ccgccaggat gagatcgatg attccgctcg
     1081 cgagcaacat gccccagcgt ccggaggtgc ggcgtcggtg atccaaagcc aacatgatcg
     1141 aggcgatacc ctcgagcaac aagaacacgg ccaatatgag cgtaagcgat acgacgcccg
     1201 acaagggcca gccgagaagt atgaaccccg cgatcacggc aagcaccgcc gaaaacaatg
     1261 accaccaaaa cccgggggcg ctgcgcatgc gcaaggtgct gatcaaaccg acgacaccgc
     1321 tcaacaacac cacccagccg atgacgatcg cagccgcgat gctggcaact gccgggagca
     1381 gcagcgcggc aatccccaga atgaggagaa agatgccctc gctgaggaac agtccccagt
     1441 gctcatgcag cgaggtgacc actgccgcct gaatcttcgt cagattctct ggattgatcg
     1501 ccatggtgga aattcctaat tgccgcgagg tttgggcact tgacccccga acatcacaaa
     1561 tacctattag accatttctt gaaatatata ataattataa gcttagagat attactgcat
     1621 aatttacttt atcatgaaag ccctctggat agatgtgttg cgcgcttaac gagcgttgcg
     1681 tctcgcggat taagtacact gcggaatcgg gtggcgcgga ttcatttcac agcgaacgga
     1741 aggttggcac gcatatggcg agaggcggcg gcagcggatt tggacttcag gatttgccaa
     1801 ggatatccca cggcaaagga tcttatcttt acgatgaggc ggggaagcgt tacatcgacg
     1861 gctccggggg cccggccgtg ttcagagtcg gacacagtca cccggaggtc aatgcggcca
     1921 tcgcacggca gatggatgcg gtcgcctacg gctatcgata tctgttcacg agctcggccc
     1981 tcgaggcctt gagcgaaatg atgctgcgaa tttccggaaa ctgcttcacc gacattgtgt
//
LOCUS       DATMGX010000029         3316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_472740, whole genome shotgun sequence.
ACCESSION   DATMGX010000029 DATMGX010000000
VERSION     DATMGX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472740"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VNR65_00480"
     CDS             complement(<1..1537)
                     /locus_tag="VNR65_00480"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding and patatin-like
                     phospholipase domain-containing protein"
                     /protein_id="HWJ17179.1"
                     /translation="MDSSSRIIPFLARIPQFSAMDAQQLTRLFGLTALKVLAKGEPAT
                     VAGGALDELRIVVSGRLAEHRKGPEAQEFGQGAALAAEAFFARRPASATLIALRETVL
                     LTLSWADLAAAFRAHPDLLATCFATFSRDRATSSPPVQPSRLVLCTAGAKGRLEVAMK
                     DAFVAALESLADVRILRRESFGSLALDAPDTAHWLQEQELEFEVTVVVADGADATFAK
                     DAIEEGDEIVFIASSGSPALSALEQYALDRRGRDRCRLLIAKDKGISLKHAADWVAPR
                     PYRSTQLVDFASPKEAALMASALLGKGIAVAATSCGVYAAAILGALQAFEASGTAPSS
                     LAAAGSAILPAGLLASGASLAETEAAFRELAEPLPWKRAAKPDTGLFEAAGVDAMLAL
                     ALPECNIGLTERPFIAVSLSLSENAPKFHREGRLHSAVRAGLTPPGVLPPFISDDNDI
                     LVSGENEVEALVAAARRLSASPLVFLYPIAPALGSSHMSYRQLAGPSFRLTSFQGLFA
                     PEQR"
     gene            1700..2062
                     /gene="rpmB"
                     /locus_tag="VNR65_00485"
     CDS             1700..2062
                     /gene="rpmB"
                     /locus_tag="VNR65_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWJ17180.1"
                     /translation="MNVNSANAPLFHSLLGPIMSRQCELTGKGGLVGHLVSHSNIKTK
                     RRYLPNLCNVTLLSDALGRGVRFRISANALRSVEHRGGLDAFLLKSKDEELSLKARRL
                     KREVVKARARTEGAAGAA"
     gene            complement(2069..2809)
                     /gene="pdeM"
                     /locus_tag="VNR65_00490"
     CDS             complement(2069..2809)
                     /gene="pdeM"
                     /locus_tag="VNR65_00490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HWJ17181.1"
                     /translation="MSIERVPSKPKTTIRGHLAEETLGLGRSSIPIIFAGLALTLDHS
                     GAAWLSTQRTLVVSDLHLEKGSSAAARGRLVPAFDSHDTLVRLRSVVEAYQPARVVCL
                     GDSFHDGRAGERMAEADRQALASLCAAVKEWVWIGGNHDPEAPEFCDGDRRDDLRIEG
                     VVLRHEPNAVRQTPEIAGHFHPKASVPAVGRRFSGRCFCISDDMLIMPAFGAYTGGLN
                     CAAQELRSLHRAEPKIFMLHASKIWRVA"
     gene            complement(2907..>3316)
                     /locus_tag="VNR65_00495"
     CDS             complement(2907..>3316)
                     /locus_tag="VNR65_00495"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17182.1"
                     /translation="RVTPVSGLTPLPALLVNPRIKLPTASVFKTLNAKPLQPEVAGPP
                     PSFPGWKNPVEAAAYLNEGRNDLEAPAIALEPAIESVLDTLRGLDGCLLSRLSGSGPT
                     CFGIFSSQEAAAEAASEIGRSYPKWWVQATTLS"
BASE COUNT          674 a         1009 c         1022 g          611 t
ORIGIN      
        1 gacgctgttc tggtgcgaaa aggccttgaa atgacgtcaa gcggaaggat ggaccagcca
       61 gctgccggta tgacatgtgc gacgatccga gcgcaggggc aattggatag agaaacacaa
      121 ggggggacgc ggagagcctt ctggcagcgg cgacgagtgc ctccacctca ttctcgccgc
      181 tgacgagaat gtcgttatcg tcggaaatga aaggcggcag cacgcccggc ggcgtcaatc
      241 cggcgcggac tgcgctgtgg aggcggcctt cgcggtggaa cttgggtgca ttctccgaaa
      301 gcgagagcga tacggctatg aacggacgct ctgtgaggcc gatgttgcat tccggcaagg
      361 ccagcgcgag catcgcgtcg acgcccgcag cctcgaacaa tcccgtgtcc ggctttgcgg
      421 ctcgcttcca gggcaacggc tcggccaatt cgcgaaacgc cgcctcggtc tcggcaaggc
      481 tcgcgccact ggcaagcaaa ccggcaggaa ggatcgcact ccctgctgct gcgaggcttg
      541 atggggcggt gccgctcgcc tcgaaggctt ggagagcgcc taaaattgcg gcggcgtaaa
      601 caccgcagct tgtggctgct acggcgattc ccttgccgag aagcgccgat gccattaacg
      661 cggcttcttt tggcgaggcg aaatcgacga gctgcgtgga gcgataaggc cgtggggcga
      721 cccaatcggc agcatgcttc agcgagatgc ccttatcctt cgcgataagg aggcggcacc
      781 tgtctcttcc ccgccggtcg agcgcgtatt gttcgagcgc cgacaaagcc ggactgccgc
      841 tcgacgcgat aaaaacgatt tcgtcgcctt cctcgatggc atccttcgca aacgtcgcgt
      901 cggctccatc tgcgaccaca accgtgacct cgaactccaa ctcctgctcc tgaagccaat
      961 gagcagtgtc cggcgcgtcg agggcaaggg agccaaagct ttcccggcga agaattctga
     1021 cgtccgccaa gctttccaag gcggcgacaa atgcgtcctt catcgcgacc tcgagccgtc
     1081 ctttggcgcc ggctgtacac aggacgaggc gggaaggctg taccggtggc gatgatgttg
     1141 ctctatccct ggaaaacgtt gcaaaacatg tcgcgagaag atcgggatgg gcacgaaacg
     1201 ctgccgcgag atcggcccac gaaagtgtca gaagcactgt ctcccgcaaa gcgatcaagg
     1261 ttgccgaggc tgggcgccgg gcaaaaaaag cctccgccgc aagcgccgcc ccttggccga
     1321 actcttgcgc ctccggtccc tttctgtgct cagcaagccg gcccgatacg acaatgcgta
     1381 gttcatcaag tgccccgcct gccaccgtgg caggctcgcc cttggccaac accttgagcg
     1441 ccgtaaggcc gaagaggcgc gtcagctgtt gagcgtccat ggctgagaat tgcgggatcc
     1501 tagccagaaa cgggatgatg cgggaagacg aatccatggc gtttccatag cgcgtggatt
     1561 ggcccgaaac aatggcaggg ccgaaaccat tgttccccta tggcgcaagg ccttaacgat
     1621 gcaacatcca caccgcgaga ggccatccac acatgggaaa aggcactcga ggcttgaccc
     1681 gcgcggcgct cggcgttaca tgaacgtgaa ctcggcgaat gcgcctcttt tccattcact
     1741 gttaggtccg atcatgtcgc gccagtgcga actcacaggc aagggtggcc tcgtcggtca
     1801 tctcgtcagc cactcaaata tcaagacgaa gcggcgctat ctgccgaacc tttgcaacgt
     1861 cacgcttttg agcgatgcgc tcggccgtgg cgtccgtttc agaatcagcg ccaacgcgct
     1921 ccgttcggtg gagcatcgcg gcgggctgga tgcgttcctt ctcaagtcta aggatgagga
     1981 gctttctctc aaggctcgcc gtctgaagcg cgaggtggtc aaggcacgcg cccggactga
     2041 aggcgctgca ggcgccgctt aagtcctctc aagcgacacg ccagattttt gaagcgtgca
     2101 gcatgaagat tttgggctcc gccctgtgga gggagcggag ctcttgtgct gcgcaattca
     2161 agccgcccgt ataggcgcca aaggccggca taataagcat atcgtcggag atacagaagc
     2221 agcggcctga aaagcggcgt ccaaccgctg gcacgctcgc cttcggatga aaatggcctg
     2281 caatctcagg cgtctgtcga acggcgtttg gttcatggcg caggacgacg ccctcgatgc
     2341 gaaggtcgtc ccgcctatcg ccatcgcaga attcgggcgc ctccggatcg tgattcccgc
     2401 caatccagac ccattctttg acagctgcgc aaagcgaggc gagcgcctgc ctgtcggcct
     2461 ccgccatgcg ttcgcccgcg cgtccgtcat gaaagctatc accgaggcaa acaacccggg
     2521 ccggctgata ggcctcgaca acggatctga ggcgtaccag cgtgtcgtgg ctgtcgaacg
     2581 ctggaacgag cctgcctctc gccgccgcgc tggagccctt ctccagatgc aagtcggaga
     2641 cgacgagcgt tcgctgggtg gaaagccaag ccgctccact gtgatcaagc gtcaaggcga
     2701 ggcctgcaaa aatgatcggg atcgaggagc ggcccaggcc taaagtctct tctgccagat
     2761 ggcccctgat cgtggtctta ggcttcgatg gtactcgctc gatgctcacg ccgctttcca
     2821 actccgtccg gtccaagcgc tcttcggacg agcgtgtaaa ttggaggcca cagacctctt
     2881 cggtggttgt ccggccatgt tccggcttat gaaagagtcg tggcctgcac ccaccacttc
     2941 ggatagctcc gcccgatctc gctcgcagcc tcggccgctg cctcttggga cgaaaagatg
     3001 ccgaaacagg tgggcccgct tcccgacaag cggctgagca ggcagccgtc caaaccgcgc
     3061 agtgtgtcca gaacgctctc gatagcaggt tcaagcgcga tggcgggcgc ttccagatcg
     3121 ttgcgcccct cgttgagata ggcggcggct tccaccggat ttttccaacc cggaaaagaa
     3181 ggcggaggtc cggccacttc tggctgcaga ggctttgcat tcaaggtctt gaagacagaa
     3241 gctgtcggca gctttatacg aggattgacc agaagggcgg gcaggggcgt taagccggat
     3301 acgggtgtca cacgtt
//
LOCUS       DATMGX010000030         4606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_24184, whole genome shotgun sequence.
ACCESSION   DATMGX010000030 DATMGX010000000
VERSION     DATMGX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24184"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(617..1663)
                     /locus_tag="VNR65_00500"
     CDS             complement(617..1663)
                     /locus_tag="VNR65_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWJ17183.1"
                     /translation="MTELIDVLPSHRFDEKKLWRFLKSHLEDFGVPPTIQQFQGGQSN
                     PTYLISSPIRKYVLRKQPSGKLLPSAHAMDREFRVQSALKGTPVPVVPMQVFCDDASV
                     IGTPFYLMDYIPGRIFDRPDLPELSTMQRKAAYRAFAETLAALHEVDFEAIGLSDYGR
                     TEGYAARQLKRWSEQYESSKTGHIEAMDKLIAWLRASVPTRDEAAITHGDYRVNNVMF
                     ELNDPHIIAVLDWELSTLGHPLADLGYVCMAYRTPASVPFLGGLRGLDLAAFGIPTED
                     ELIGMYCHRAGRDSVPDLTFFIALSFFRFASIAQGVYARSLQGNAADKRASLAEHAAI
                     ALAQEGWRIAEAAG"
     gene            complement(1670..2443)
                     /locus_tag="VNR65_00505"
     CDS             complement(1670..2443)
                     /locus_tag="VNR65_00505"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ17184.1"
                     /translation="MSMFDLTGRVAVITGSSRGIGRAIAIECARMGASVVVSSRKIEA
                     CKAVVAEIRDAGGHATAVACNVGHKPDLECLVAHALREYGHIDILIPNAAINPVYGPS
                     SEVSDEVWNKVLTTNLTSTHWLCQLVLPGMAEHGGGSVIMLSSIVAIAGAANLGVYAV
                     SKAAEAQLARNLAVEWGTRGIRVNSIAPGIVKTDFAKALYEDPRTAALVANLTCLKRL
                     GEPADIAGVAVFLASNAARYITGQMILVDGGASIFTPIG"
     gene            complement(2454..4541)
                     /locus_tag="VNR65_00510"
     CDS             complement(2454..4541)
                     /locus_tag="VNR65_00510"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWJ17185.1"
                     /translation="MAAIPVNYANKDGIGIIIVNNPPLNALSREVRDGLLAAIRQAEA
                     DESAHAIVLTGAGQTFSAGADITEFDSPLKGTEINQICDAIEDLHKPVVAALHGTSLG
                     EGLEIALACHYRIASKDARLGLPEVKLGLIPGGGGTQRLPRLTGAKAALDLIISGDPI
                     SADAARKAGIVDEIAEGDHLAAAMALAKAKAGLASHPKTREQNAKIEAERGSPIFDEK
                     RAELKKRQQHQHAPLRCVAAVESAFKLPFEEGLRREGELFYEALHDWQSKALIHAFFA
                     ERHVVKIPGLSSKFKPKEIKSAGVIGTGTMGGGIAMAFANAGIPVVVLDRDQAALYKG
                     LGVVAKNYESMVQRGRLDQAGMDARMAAIKPTLEYGALADVDIVVEAAVEDLGVKAQV
                     FRELDRVCKEGAILATNTSTLDIDKIASFTSRPADVVGMHFFSPANVMRLVEVVHGKE
                     TSAEVLGSAIAAARRIGKIGVSVGNCDGFVGNRMLEKYITEAITIVEEGASPAEVDAA
                     LTGWGMAMGPFAVFDLTGIDVNWHIRQRRLFEGRDYASPLLDRFYEAGRYGQKTSKGF
                     YRYEPYGRIPLPDPEADTIIEAYREELGNRIGSVSEQEIVKRTIGALINEGANILDEG
                     IAYRADDIDIIYIHGYGFPAWRGGPMKYAELLTLPEVLSHIDMFHRRFGDRWRPAPLL
                     KALVTERKPRWPR"
BASE COUNT          875 a         1395 c         1388 g          948 t
ORIGIN      
        1 atggaattta gtgccggaat tttttccttt actgggacgc gctaaagtcc gttaaatatg
       61 ttcccgccgc gcctgttaac catgtccgcg cctctcgcag cgcggcggcg tcgtcggcgt
      121 cgtcatgcgt ccttcaattt atcttcgata aatcaaagac ttgtctcaat taacctcccg
      181 gcaaggagct ttattctagg atccctgcaa gccagaatga cagctggttt tcgttgaatg
      241 cgcgctcgat tttggtccaa gccacaattg ttttggacca ttgtctggtc catgaggaaa
      301 cggccatgca gatttcagga cccgatggtt ggcataaggc ctgattgctc gctggatggc
      361 tggtccgtgg atcgagcccg gccacgacag cttgagcgca caaccgcgag gcggcgatgg
      421 cgttcgttcg gtgtttttcc cgcaatcatc aagggttgga gcgggctggg ctctcgttaa
      481 cgttaacctg ccggcaaggc gatttcgctg acgatcttgc catccggacg cccgcgttcg
      541 attgaacgcg cgctctacag gatggcctgg aaatgctccg attgatgata tgagccgaac
      601 gcattcactg ttggtactac ccggcggctt ccgcgatgcg ccagccctct tgcgccagag
      661 cgatggcggc atgctccgcg agcgacgcgc gcttgtccgc ggcattgcct tgcagcgagc
      721 gggcataaac gccctgcgcg atcgaggcga agcggaagaa cgaaagtgcg ataaagaacg
      781 tgagatcggg gacgctgtcg cggcctgcgc ggtggcagta catgccgatc agttcgtcct
      841 ctgtcgggat gccgaacgcg gccaggtcca gtccgcgcag gccgcccagg aatggcacgc
      901 tggccggtgt gcgataggcc atgcagacat agccgaggtc ggcgagcgga tggcccagcg
      961 tcgacaattc ccaatcgagc accgcgatga tgtgtggatc gttcagttcg aacatcacat
     1021 tgttcacgcg gtaatcgcca tgcgtgatcg ccgcttcgtc gcgggtcgga acgctcgccc
     1081 tgagccaagc gatcagcttg tccatggcct cgatgtgacc ggtcttcgag gactcgtatt
     1141 gttcgctcca acgcttgagc tggcgagccg catagccttc cgtccgtccg taatcgctga
     1201 ggccaatcgc ttcgaagtcc acctcgtgaa gcgccgcgag cgtctcggcg aaagcgcgat
     1261 aagcagcctt gcgctgcata gtgctgagtt ccgggaggtc cggtctatcg aagatgcggc
     1321 cggggatgta gtccatcagg tagaacggcg tcccgatgac cgaggcgtcg tcgcagaaca
     1381 cctgcatcgg gaccacggga acgggcgtgc ccttcaacgc tgactggacg cgaaactccc
     1441 ggtccatggc atgcgcgctc ggcaagagct ttcctgaagg ctgcttgcgc aggacgtact
     1501 tccggatggg cgagctgatg aggtaggtcg ggttggattg gccgccctgg aactgctgaa
     1561 tggttggcgg aacgccgaaa tcttccagat ggcttttcaa gaagcgccag agcttctttt
     1621 cgtcgaagcg atgacttgga agaacgtcga tgagttcagt cacgatgact tatccgattg
     1681 gagtgaagat tgaagcgccg ccgtccacga ggatcatttg gcctgtgata tatcgcgccg
     1741 cgttggaagc caagaacact gcgacgcccg cgatgtcggc cggctcgcca agccgcttca
     1801 ggcacgtcaa gttcgcaacc agggccgccg ttcttggatc ctcgtaaagc gccttggcga
     1861 agtcggtctt cacgatgccg ggcgcgatcg aattgacgcg aatgccgcgc gttccccact
     1921 ccacggcaag gttgcgggcg agttgggcct ccgccgcctt cgacacggca tagacgccga
     1981 gattggcggc tcccgcgatc gcgacgatcg aggacagcat gataaccgag ccgcccccgt
     2041 gctcggccat gcccggcaga accagctggc acagccaatg cgtcgaagtg aggtttgtgg
     2101 tgagaacctt gttccagacc tcgtcggaga cttcggacga cgggccgtaa accgggttga
     2161 tggccgcgtt cgggatcagg atgtcgatgt gaccgtattc acggagagca tgggcgacga
     2221 gacattcgag atcgggctta tggccgacat tgcacgccac cgccgtcgca tggccaccag
     2281 cgtcgcggat ttcggcgacc acagccttgc aagcttcgat cttacgggag gaaaccacaa
     2341 cgctcgcacc catcctcgcg cattcaatcg cgatggcccg gccgatcccg cgagaagagc
     2401 ccgtaatgac ggcaacgcgc cccgtaaggt cgaacatact catccagcac ccatcatcgc
     2461 ggccaccgcg gcttgcgttc tgtcacgagc gccttgagca gcggcgcagg ccgccagcga
     2521 tcgccaaagc gccggtggaa catatcgata tgcgataaga cttccggcaa ggtcagcagt
     2581 tccgcatatt tcatggggcc gccccgccat gcgggaaatc catatccgtg aatgtagatg
     2641 atatcgatgt catcggcgcg gtacgcgatc ccttcgtcga ggatgttcgc gccctcgttg
     2701 atgagcgcac cgatcgttcg cttcacgatc tcctgctcgg agacggagcc tatcctgttg
     2761 ccgagctcct cgcggtaagc ttcgatgatg gtgtccgcct cagggtcggg caagggtatg
     2821 cggccgtaag gttcgtagcg atagaagccc ttgcttgttt tctgcccgta gcgaccggcc
     2881 tcataaaagc gatccagcag gggcgaggcg taatcccgcc cctcaaaaag gcgtctctga
     2941 cgaatatgcc agttcacgtc gatccccgtc agatcgaaga cggcgaacgg ccccatggcc
     3001 atgccccagc cagtcagcgc cgcgtcgacc tcggctggag acgccccctc ctccacgatg
     3061 gtgatggcct cggtgatata tttctcgagc atgcgatttc cgacgaagcc gtcgcagttc
     3121 ccgaccgaaa cgccaatctt gccgatcctg cgcgccgctg ctatggcgct gcccagcacc
     3181 tccgcgcttg tctccttgcc atggacgacc tcgacaagcc gcatgacatt cgcgggcgag
     3241 aaaaaatgca tgccgaccac gtcggcgggg cgcgacgtga aactcgcgat cttgtcgata
     3301 tcgagcgtgg aggtgttggt ggccaggatc gcgccttctt tgcagacgcg gtcgagttcg
     3361 cgaaagactt gggctttcac gcccagatcc tcgacagccg cttccaccac gatgtcgaca
     3421 tccgcgagcg ccccatattc cagggtgggt ttgatcgcgg ccatgcgggc atccattccg
     3481 gcctgatcga gccgcccgcg ctgcaccatg ctctcgtagt tcttcgcaac aacgccgagc
     3541 cctttgtaaa gcgcggcctg atcgcggtcg agaacgacaa caggaatccc ggcattcgca
     3601 aaggccatcg cgatgccacc gcccatcgtc cccgtcccga tgacgccagc gctcttgatc
     3661 tctttcggct taaacttgct ggaaaggcct gggattttga cgacgtggcg ctccgcgaaa
     3721 aacgcatgga tgagcgcttt gctttgccag tcgtggagtg cctcataaaa cagctcgccc
     3781 tcgcgcctca gcccttcttc aaacggaagc ttgaatgcgc tttcgactgc agcgacgcag
     3841 cgtagcggcg catgctgatg ctgctggcgc tttttgagct cggcgcgctt ttcgtcgaag
     3901 atgggcgaac cgcgctcggc ttcgattttt gcgttttgtt cgcgtgtctt gggatggctc
     3961 gccaagccag ctttggcttt agccaaagcc atcgcggcgg ccagatgatc gccttccgcg
     4021 atctcgtcca caatccccgc tttccgggct gcgtcggccg aaatcggatc gccggatatg
     4081 atgagatcaa gcgcggcctt cgcccccgtc aggcgcggca ggcgctgagt gcctccgcct
     4141 ccgggaataa ggccgagctt cacctccggc aagccgaggc gggcatcttt ggatgcgatc
     4201 ctgtaatggc aggcgagcgc aatctccagt ccctcgccga gcgaagtgcc atgcagcgct
     4261 gcgaccacag gcttgtgcaa gtcctcgata gcgtcgcaga tctggttgat ttcggtccct
     4321 ttgagggggg agtcgaattc cgtgatatcg gccccggcgg agaaggtctg acctgctccg
     4381 gtgagaacga tagcatgggc gctctcgtcg gcttcagcct gcctgatggc ggccaggagc
     4441 ccgtcgcgaa cttcccgcga cagcgcattg aggggaggat tattgacaat tatgatgcca
     4501 atgccgtcct tgttcgcgta gttaacggga atcgcggcca tgaatactcc cgagcggccg
     4561 ctatttgctt cttaagttcg gcctcgatga acaatacagt tcgctg
//
LOCUS       DATMGX010000031         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_472635, whole genome shotgun sequence.
ACCESSION   DATMGX010000031 DATMGX010000000
VERSION     DATMGX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472635"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VNR65_00515"
     CDS             complement(<1..459)
                     /locus_tag="VNR65_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWJ17186.1"
                     /translation="MQRITAINPAEATGKTKKLLDGVQAKLGMTPNLMKTLASAPAAL
                     EAYLNFGAALGTGALDAKFREQISIAVAQANSCEYCLSAHSTIGKMVGLTPEEIAGSR
                     ESHAGDAKRNAGLQLAQAIVVQRGEVSDAALANARAAGYTDGEITEIVANV"
     gene            complement(981..2096)
                     /locus_tag="VNR65_00520"
     CDS             complement(981..2096)
                     /locus_tag="VNR65_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17187.1"
                     /translation="MQEITPIRLAEATGKTRPAMLLLMCAVTISAAFGGEEPQAQAKQ
                     GRNQDPLFLTATNGTNNFLAVINTRTRQTDFVPTGGFGGAGGNAGGVAVNEQIAAVVN
                     FASSNVTIFARRGNAMEPVKIIKTSSQPVSVAFGHNHLVVLGLTTAESFPVYEDTVQA
                     ASDGVVQLSRGDKSAAQIVTYDGGALYSEKSGSLAGLDLSTNGIAGLSGPNRPVLLPT
                     APNNDTPFGMVARGANVYVTIAHSDLEALVVNGGIIATAAGPTPYKNQSGGILHAPCW
                     NALSGQFLFSADSPGKQLLRYLVSDNSVFFDKPGVATFTGAPTDLAVEGSLLGVIDGG
                     DGSTSNASLFDIDSEGELRLRFAVKVAGRINGAAIVN"
BASE COUNT          472 a          723 c          772 g          532 t
ORIGIN      
        1 gacgttagct acgatttcag tgatctcgcc gtcggtgtaa ccggccgcgc gagcgttggc
       61 cagcgccgca tcggatacct ctccgcgttg caccacgatg gcctgcgcga gttggaggcc
      121 tgcattgcgc ttggcgtctc cggcatgcga ttcacggctg ccggcgattt cctccggtgt
      181 caggccgacc atcttgccga ttgtggaatg cgccgacagg cagtactcgc aggagttcgc
      241 ctgggccacc gcgatggaaa tctgctcacg gaacttggcg tccagggcgc ctgttcccaa
      301 cgcggcaccg aaattgaggt aagcttcgag cgccgccggc gcggaggcca gcgtcttcat
      361 gagattgggg gtcatcccca acttggcttg gacgccgtcc aggagtttct tggttttgcc
      421 ggtggcttcg gcgggattga ttgcggtgat tcgttgcatg ctgtgtaggc tcctagtttt
      481 gaatcgcgaa agccgcgaca ccgggattaa tgcaggcgcc atgccaaaac cgatccggtc
      541 aaagtcattg caaacaatag gaaagagcgt taggcggatt tgagaaactt cacgatttac
      601 gatttctcac aattggattc atgaagcctc gcggaagtgt tcacgcactc aaggcggcga
      661 ccccttggca gaccccctcg actcggtatg cgggggccgg ccggaacagt cggactaggg
      721 ggggcgcatg ctgggtgcag gggagttcgc tgaagcgcgg caacctgccg tccgcagagt
      781 ggggacttcg ggggtatcgc ttggaacgcg aaaagaggat tcggacgcgc atcccaggtg
      841 cgccatctgg agactctgac gacagaggga gcccggctcc ccgcggcaga cctggaaacg
      901 gtatcgcgca gattggggac cgcgatccaa ctgcggtccc ccggcatttc gcgattcggg
      961 tcagttcaaa ctgccggcag ttagttgacg atcgcggcgc cattaattcg acccgcgacc
     1021 tttacggcaa aacgcaggcg gagttcgccc tcggaatcga tgtcgaatag cgaggcgttg
     1081 gaggtgcttc cgtcgccacc atcgatgacg cctaacaggc tgccctcgac agccaggtcc
     1141 gtcggcgccc cggtgaatgt agcgacgccc ggcttgtcga agaagacgct gttatcactt
     1201 accagatatc gaagtagttg cttgcccggg ctgtccgccg agaacaagaa ctgcccggat
     1261 agagcgttcc agcagggagc gtggaggata ccgccagact ggttcttgta cggcgtcggg
     1321 ccggccgcgg tagcaatgat tccgccattc accaccaagg cctccaggtc cgagtgtgcg
     1381 atggtgacat acacgttggc gcctcgtgcc accatgccga agggggtatc gttattaggc
     1441 gctgtcggca gcagaacggg gcggttcggc ccggataggc ccgcgatccc gttggtggat
     1501 aaatccaatc cggcaaggga tccactcttt tccgagtaca gtgcgccacc gtcgtaagtc
     1561 acgatctgcg ccgcgctctt gtcgcctctt gagagctgaa ccacaccatc gctcgcagcc
     1621 tgcaccgtgt cttcgtaaac ggggaaggac tccgcggtgg tgaggcccag caccaccaga
     1681 tggttatgcc cgaaagcaac agacaccggc tgcgaagatg ttttgatgat cttgactggt
     1741 tccatcgcgt tgccccgacg ggcgaagatc gtgacattgg aagaggcgaa gttgacgacc
     1801 gccgcgattt gttcgtttac tgctacgcct cccgcgttgc cgcctgcccc cccaaacccg
     1861 cccgtcggga cgaaatccgt ttgtctggtt cgggtattga tgacggccag gaagttgttg
     1921 gtgccgttgg tcgcagtcag gaacagtgga tcctggttac gtccctgttt ggcttgtgcc
     1981 tgtggctctt cgccgccgaa ggcggccgat attgtgacgg cgcacatcaa cagcaacatg
     2041 gccggtctgg ttttgccggt ggcttcggcg agacggattg gggtgatttc ttgcatgttc
     2101 tataggctcc tttttcaatc gcgaaagccg cgacgcttcg gactagtgca ggtgccgcgc
     2161 caatgcttga gggcttcaaa taactgaaac aattcgacaa cagagttcag cgaatttgcg
     2221 atacctcacg atttgcgatt tctcaccatc aaatttctgc aatgtgggcg atgctagact
     2281 gagctatgaa tctggcgccg ctaccgtcga tcattctggc catcgccgag cagcggtcct
     2341 tgtgtgctgt actggcgacg atcgtcgact ctgttgcgcg gcaaccaaac gtggccctcg
     2401 cccgtctgtg gttacggcaa tctgatcgcg actgccctca ctgttcccgc tccgcccagg
     2461 attccgggga gtcactccac ctccgggcga gttctggga
//
LOCUS       DATMGX010000032         2713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_406489, whole genome shotgun sequence.
ACCESSION   DATMGX010000032 DATMGX010000000
VERSION     DATMGX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406489"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..2549
                     /locus_tag="VNR65_00525"
     CDS             354..2549
                     /locus_tag="VNR65_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17188.1"
                     /translation="MKKISLAATDRHIERYIVRHLTKVLTAVGKCEPAQVALASRVKL
                     DTESALASEYGGTLVALVELVTLLSLANQNDVAQPPHGDADRTLHVANAIKHAQHLIA
                     AGVGRSALEIDPDTPTWQLGLIGRGREVFLRATVYDRMLAETLRILFNDEQANIRART
                     AVGFGGKDALDAIIVFKQLHTDRLQALARRYEYVQTRITSLLRASKDVLDPNLDEDFV
                     KEAEGAVWDITEGLAQACSISYPEVAEASGVPYDTVHHILDAFTISLDGSPREMFERF
                     IAGDDPFRTQPIIRRPDGTMVLIHESLAMSAIRERFEASIRSHKTLWEQYDKTRGQVA
                     ETIALDALESCLGDVECHRSLFYFVPDPKAEANQTRPDNYTKRVEVDGLILMDDVALI
                     VEVKAVALTPESRSGPDRAVAGKLNSIVSRAAEQGFRLYDRIFEDGELKIGKGKKINL
                     QRIREAYVITVSLEDLSGTLVSIDGLIDAGVVDQQRLPWTISLHDLKIVCALIEHPSQ
                     LLLYLRRRTDRAIMSQFLAMDEIDIFVLFLDGLFFKHGDVLHREMNDLWPKKSLHRFR
                     RPRRPILYAIGSRTEPVDRWMEGLNVPGSFPAEPPRMEIDAFVRRTLDVLSELREFGW
                     VSTAAALLEGSPSLQRQFALIGPALLEKLSKDHKDHYHGLVLNGSAGMKLVLLWYVSQ
                     HRFDRGKLMTIRRRLLRLSQEHGARSCSCLYIGATNGEVEALVYWPIED"
BASE COUNT          626 a          695 c          802 g          590 t
ORIGIN      
        1 gcccgtgcgg aggcatcgct ggtcgggtgg acaaaggggc ggtgatcggc gctctgtgag
       61 gttggcacac ctctcggata cagacgccag gagccgggcg tggacgctgg ggtcgatgca
      121 gcatggtgac tggcctccgc atgggtcccg gacggcgcgc gagcagatac aaggctccgg
      181 aggttcggta gtttaacgcg gtccgattgc cgattgtggc ggtagacgaa gttgaggagg
      241 ttcgtgtggt tgcaggggca acggatggag gagcgagcgt cggggggccg cgtcgcggtc
      301 cggctgcatc actcgggcgg gagagcgtgt caaggacgat gagatatcta tggttgaaga
      361 agatttcact cgccgcaacg gatcgacata tcgagcgcta tatcgtcaga catctgacga
      421 aggtgctgac agcagtaggt aaatgcgagc cggcgcaggt ggctctcgca tcacgagtga
      481 agctcgatac cgagagcgcg ctagcatccg aatacggcgg gaccctcgtt gcactagtcg
      541 aactggtgac ccttctttcg ctcgcgaatc agaacgacgt tgcccagcca ccacacggtg
      601 atgccgaccg cacgcttcac gtggccaacg cgattaagca tgcgcagcac cttatcgcgg
      661 cgggcgttgg gcggtccgcc ctcgaaatcg atccagatac acccacctgg caattgggac
      721 tgattgggcg agggcgagaa gtatttctcc gtgccaccgt gtacgacagg atgcttgcag
      781 aaacgttgag gatactcttc aacgatgagc aggccaatat ccgtgcacga accgcagtgg
      841 gctttggcgg caaggacgcc ctcgatgcaa tcattgtatt caagcagctg cacaccgatc
      901 gccttcaagc tttagcccgt cgctacgaat atgtgcagac gcggataaca agtctgttga
      961 gggcgtcaaa ggatgtcctc gaccccaatt tggatgaaga cttcgtgaag gaagcggagg
     1021 gcgctgtatg ggacatcacc gagggtctcg ctcaagcgtg ttcaatttcg taccccgagg
     1081 tggcggaggc atcgggcgtc ccatatgaca cagttcacca catacttgat gcctttacca
     1141 tttcactaga tggctcacca agagaaatgt tcgagcgatt catcgcgggc gacgacccgt
     1201 ttcgaacaca accgataata cgtcgcccag acggtacgat ggtgctaatc catgaatcac
     1261 ttgcgatgtc agccattcga gagcgatttg aggcgtccat aagaagccat aagacgctgt
     1321 gggagcagta cgacaagact cggggccaag tggccgagac gattgccttg gatgcccttg
     1381 aatcgtgcct cggcgatgtc gagtgccacc gctccctctt ctacttcgta cctgatccca
     1441 aagccgaagc caatcagacg cggccagaca actacaccaa gcgcgtcgag gtcgacggtc
     1501 taattttgat ggatgatgta gcattgatcg tcgaagtcaa agcggtagca ttgacgccgg
     1561 agtcacgaag tggcccagac cgtgcagtgg ccggcaagtt aaattccata gtatctcggg
     1621 cagcagagca aggcttccga ctttacgaca gaatatttga ggatggcgaa ctcaaaatcg
     1681 gcaaaggtaa gaagatcaat ttacaaagaa ttcgcgaggc ttatgtaatc acggtaagct
     1741 tggaggactt gtcgggaacg ctggtttcaa tcgacggttt gatcgatgct ggtgtggtcg
     1801 atcaacagag gctaccttgg accatctccc ttcacgacct gaaaatcgta tgcgcgctaa
     1861 tcgaacaccc ttctcaactg ctactatatc ttcggcgtcg cactgataga gccattatga
     1921 gccagtttct agcgatggat gaaatcgaca tattcgtcct gttcctggac gggcttttct
     1981 tcaagcacgg cgacgtcctg catcgcgaga tgaacgacct gtggccgaag aaatcgttac
     2041 accgcttccg taggccgaga cggccaatcc tctatgcgat agggtctcgt acagaaccag
     2101 tagaccgctg gatggaagga ttgaacgtac ccggttcttt cccggcagag cccccacgaa
     2161 tggagattga cgcgtttgtg cggcggactt tggatgtcct atccgagctc cgcgagtttg
     2221 gttgggtttc aacggccgcc gcgctcctcg aaggatctcc cagtctgcaa cgtcagttcg
     2281 ccctgatcgg accagcgcta ttagagaaac tttcgaagga tcacaaggat cactatcacg
     2341 gactagtctt gaatggttcg gcagggatga agttggtcct attgtggtat gtctcccagc
     2401 ataggtttga cagaggcaag ctgatgacaa ttcgaagacg tctacttcgt ctttctcagg
     2461 agcacggagc aaggtcgtgc tcgtgcctct acatcggtgc tactaacggc gaggttgaag
     2521 cgcttgttta ttggccgata gaggactagt ttaaatcagc gagcattagg atctcaagtt
     2581 tcgcagtttt gaggccgtgg aacaccgaat tgcggagtca ccgaacgaat gaaactctgt
     2641 tgtcctaaat tgaatgtcat tccgttcggc gcgcctgccg agcatttcat gggatggggc
     2701 ggaacattgg tgg
//
LOCUS       DATMGX010000033         7519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_23873, whole genome shotgun sequence.
ACCESSION   DATMGX010000033 DATMGX010000000
VERSION     DATMGX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23873"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VNR65_00530"
     CDS             complement(<1..489)
                     /locus_tag="VNR65_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HWJ17189.1"
                     /translation="MRRVISVFLPRWPTDRLRRKNRTAPPRDKPLVLAAVDGQRRIVT
                     SVDDAALSLGLTRGMTVTHAQSLVPDLTVLDADPAKDADALVRLGHWCTIYSPLVTPD
                     PPDGIFIDVAGSAHLFQGEGALIEDLLHRLRRADISAKAAIADTPGCAWGVSRYGSGG
                     IVS"
     gene            complement(404..1165)
                     /locus_tag="VNR65_00535"
     CDS             complement(404..1165)
                     /locus_tag="VNR65_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17190.1"
                     /translation="MAPASPPTSIAELRRWLERVEGNGYATSEALPFAVTEIDQHLPG
                     GGLARGHLHEVIEGGPAGEHAAVATLFAAGIAARIPGPVLWCLRGRDLFAPALARIGL
                     HPDRVIYCETWKDRDVLPAMEEGLRCCGIAAVVGEVTRLSLTSSRRLQLCAGETGVTA
                     LLLRRWRTSDEKRFAGEPNAAATRWRVSPSLPAQGPSDHIDRQRWLVELLRVKGGEPH
                     SWIVEACDAEGNLRLPATLADRSAAAEESYRAAAG"
     gene            complement(2314..3855)
                     /locus_tag="VNR65_00540"
     CDS             complement(2314..3855)
                     /locus_tag="VNR65_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ17191.1"
                     /translation="MPALRTRILYIDDDPGLCRLVQKDLERQGYVVEIATDGASGVAR
                     IAQGGIDVVALDHYMPNQDGLETLASIRNLAEPPPVIYVTAMQEGRVAVAALKAGAAD
                     YVVKDVQGEFLALLQRAIDAALDAAMLRRAKEAAEAEVRAARDRFEALANERALLLRE
                     VNHRVSEELAARAESKRNWQLTQSIVDTIRDPLVVLENDMTIVTASTAFLTIFGITEA
                     EAHGRRVSELGQHQWDVPALRHLMEKVLPENKPIESFEIEDNFPGLGRRVFNLNARKI
                     SQPGNHAHRMLLVFEDITDRKQRERDALSLTNEISHRIKNNLQVIVGLIAYESKWTAA
                     PCVQGYKAMQARIGAIAQLYDLISQTSRGRTIAVDSYLREIAKAMSASVLGNTSGIEI
                     EVKAEPLEIDPERAVPFGLLVNELTTNAIKHAFPEGKGHIMLCVEQVGDQIELVVADD
                     GVGMKDKDSAKGPEKRGSDYVAIFVRQLGGMIVPSQSEGAGTIVRIRLPLLLVPPGGA
                     ERVAA"
     gene            complement(3855..4292)
                     /locus_tag="VNR65_00545"
     CDS             complement(3855..4292)
                     /locus_tag="VNR65_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ17192.1"
                     /translation="MTQPVTIAMIEDDEGHARLIEKNLRRAGVHNEIVRLADGASAIT
                     FLFGSDGSGLVNKGRALLILLDLNLPDMSGVDILKRLKENEHLRVSPVVVLTTTDDKS
                     EIQRCYDLGCNVYITKPVNYENFANAIQQLGLFVYVMQVPETI"
     gene            complement(4289..5779)
                     /locus_tag="VNR65_00550"
     CDS             complement(4289..5779)
                     /locus_tag="VNR65_00550"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWJ17193.1"
                     /translation="MTPPTVSRLVRREPIFLALGFVILITISAISIWFVKQVEFGADQ
                     VAHTLEVRNKLANLRLLLRRSESSQRGYLLTSDLGYLATYQDDMKAIAPALDELSSTT
                     SDNPRQQDFLAGLRPAVNAKLDELRETVRLYQSGDAAGASALVGATGLTLMQEILTVI
                     GRMENEEQRLQNVRTAALDNSSRWLLIVTLTGSILILVLAATSVLLLRHSLKEQQDAN
                     RMLEATNQNLETIVAKRTALLSEANEEVQRFAYIVSHDLRSPLVNIMGFASELEAWRK
                     QIFERIGALRAQVGGEQDSESDEALRSDVEEALSFIKSSIAKMDRLIGALLKLSREGQ
                     RQFQPQFIDMSQLLRSALEAMAHQAQDVNAKIIIGELPTITSDRLALEQVFSNLMDNA
                     VKYLRAGVPGSIEVRATESDGAITYEVHDNGRGIDAKDRERIFDLFRRSGIQDRPGDG
                     IGLAHARALVRRLGGTINVESEPDRGTVFKVTLPKRWPLPVSEMMT"
     gene            complement(5776..6051)
                     /locus_tag="VNR65_00555"
     CDS             complement(5776..6051)
                     /locus_tag="VNR65_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWJ17194.1"
                     /translation="MPLSDIAASDIVATAPSALIVLDQNLNITSADRAFYQTFRTSPD
                     ETEGLTYYGNAAMMPIDFRTEPRLVKPFDHAKLAGLVAEHFRRHGTR"
     gene            complement(6082..6447)
                     /locus_tag="VNR65_00560"
     CDS             complement(6082..6447)
                     /locus_tag="VNR65_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ17195.1"
                     /translation="MAVILIVEDDVFIREIAEMMIQDWGHKTLSASDVDEALLLLRSP
                     QHIDALFTDIYLKTAVLGGCDLAPQAIKLRRNLRVLYTTGNSITDKMKALFVKGSHFL
                     CKPYTQYQLQNSVENLLAA"
     gene            complement(6658..6959)
                     /locus_tag="VNR65_00565"
                     /pseudo
     CDS             complement(6658..6959)
                     /locus_tag="VNR65_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510130.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6968..7120
                     /locus_tag="VNR65_00570"
     CDS             6968..7120
                     /locus_tag="VNR65_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17196.1"
                     /translation="MSNSDVTAVIAMDGVDAECHAIALPSRPGQAAPISSQEPFAPPA
                     FLTMAA"
BASE COUNT         1551 a         2252 c         2139 g         1577 t
ORIGIN      
        1 cgacacgatc ccgccactcc catagcggga caccccccac gcgcagcccg gcgtgtctgc
       61 gatcgcggct ttcgccgaga tgtcggcgcg tcggaggcga tgcagcagat cctcgatcaa
      121 cgcgccttcg ccctggaaca aatgcgccga tcccgctaca tcgatgaata ttccatcggg
      181 cggatcgggt gtgacgagcg gcgaatatat cgtgcaccag tggccgagcc gcaccaatgc
      241 atcggcatcc tttgccggat ctgcgtcgag aaccgtcaag tcgggcacga gcgattgcgc
      301 gtgcgtgacc gtcatgccgc gcgtcaggcc aagtgacagc gccgcatcgt ccacgctcgt
      361 gacgatgcgg cgctgaccgt cgacggccgc gagaaccagc ggcttatccc gcggcggcgc
      421 ggtacgattc ttccgccgca gccgatcggt cggccagcgt ggcaggaaga cggagattac
      481 cctccgcatc gcaagcctcc acgatccatg aatgcggttc gccgcccttg acgcgcagca
      541 gttcgacaag ccagcgctgc cgatcgatat gatcggaggg accttgagcc ggcaggctgg
      601 gcgatacgcg ccagcgcgtg gccgcggcat tgggctcgcc ggcaaaccgc ttctcgtcgg
      661 aagtgcgcca cctccgtaga agcaacgcgg tgacgcccgt ttcacccgcg cacaactgca
      721 ggcgccgcga cgacgtcaaa gatagccgag tcacctcgcc gacgacggcg gcaatcccgc
      781 agcagcgcag cccttcctcc atcgcaggaa gcacatcgcg gtctttccag gtctcgcagt
      841 agatgacgcg atccggatga agcccgatgc gggcaagcgc gggcgcgaac agatcgcgcc
      901 cgcgcaggca ccacagcacg ggtccgggaa tccgcgcggc gatgccggct gcgaacagtg
      961 tcgctaccgc ggcatgttcg cccgccggtc cgccttcgat cacttcatgg aggtggccgc
     1021 gcgcgaggcc gccacccggc aaatgctgat cgatctcggt cacggcgaac ggcaacgcct
     1081 ccgacgtcgc atagccgttt ccttccaccc gctccaacca gcggcgcaat tcggcaatag
     1141 aagtaggcgg gctggccggg gccatccaca gagtctcctt gggaaattgt ctgttctaca
     1201 tttgttctct tcgagggaag agtcaactgc cttgcgaaat agctcggtct tactgcctga
     1261 tcgagccgaa ctttcccact ttcgccgaca tcgcgttggc tgtagtcgaa tccatggaag
     1321 ccgcgtgacc gttccgaagg acgcaggttg ctcatgcatg tgaatgccga ctagcggcgc
     1381 gggcttcggc tcgatcacgc gaataaccag ctcggcttcg ggcgaacgcg ggatgaaatt
     1441 gagcgtcacg ccatagccct tgccgtcgcg gtggggccac gcggcaccga tttcgagcca
     1501 ggtgcctttc tcgcggcctt ccttcttgat gaccgcgcaa acgcgatgag tcgggcggtt
     1561 gctgttggtt tcagtcgtca tggtgttgtc ctttcggtac tgagcccgcc acgatggcgg
     1621 actcgatgcc gctcacgggc cgaagcgttc aagcggtgct gtcacaacct ggccgccagg
     1681 ccacccgaag ggcgtggagc tgcacaagct gcaggcgcgg aagcgaacgg ctttgatcag
     1741 cagcgcgcgc ttcggcagaa gacgtgcaat tcaagaggac gcttggcggg acttcctgca
     1801 acgaaaagac gatgacgaca gaaccgtgct cacgccctgt tgtcggcagc cggtcaattc
     1861 gagaaggcga ggaagattgg agggcagtga atccacctcg ttcgggacga gggggtcgca
     1921 ggttcaaatc cttccactcc gaccagctaa caaattgatt ttgcaggctt tcccgaaaag
     1981 cgctagtggc cgaattttct accgccacta aaaccgccac tgaaacgact tggccgacgc
     2041 cgttccgcca gcttaagggg ttcggtcacc tcatcatcaa cgcggatgag gttttcggca
     2101 cacacaggcg cccgatatcg atcttctgac cgccgtggac gtcgccattc gcgatcttca
     2161 agacatttcg caaggctgcg gcgcttcggt ttgcgagcag gttatgcagt gtcggcaaat
     2221 gcttgagcga gcattcaacg ccgcggtcca gtcacccaga cagactcgag aaatcgcact
     2281 gatgacttcg tcgccgatca gcctgtaagc gcgctatgca gctacgcgct cggcaccccc
     2341 tggtgggacc agaagcaagg gaagtcgtat tctaacgatc gttccagctc cttccgattg
     2401 cgacgggacg atcatgccgc cgagctgacg cacgaagata gccacgtagt cggaaccacg
     2461 tttctccgga cctttcgccg aatccttatc cttcataccc acaccgtcat cggcaacaac
     2521 cagctcaatt tgatcgccga cctgttccac gcacagcatg atatgtccct tcccttcagg
     2581 aaaagcatgc ttgatggcgt tcgtcgtaag ctcattgacc agaaggccga agggtacggc
     2641 acgctcgggg tcgatttcca acggctcggc cttaacctct atttcgatgc ccgacgtgtt
     2701 tccaagcacg ctcgccgaca tcgccttggc tatttcccgc agatatgagt ccacggcgat
     2761 ggtccggccg cggctggttt gagatatcag gtcgtaaagc tgggcaatgg ctccaatgcg
     2821 tgcctgcatg gctttatagc cttgaacgca cggagctgcc gtccatttgg attcgtaggc
     2881 gatcaagccg acgatgacct gcaagttatt cttgatccgg tgagagattt cgttggtcaa
     2941 gctgagcgcg tcgcgctcgc gctgcttgcg gtcggtgatg tcctcaaaga cgaggagcat
     3001 ccgatgggca tggttccccg gttgagaaat tttccgagca ttcaagttga agacacggcg
     3061 gccgagaccc ggaaaattat cctcgatttc gaaactttcg atcggcttgt tttccgggag
     3121 caccttctcc atcaaatggc gaagcgccgg tacgtcccat tggtgctgac ctagttcgga
     3181 aacgcgccgc ccatgcgcct ccgcctcggt aataccgaat atcgtcaaaa aagccgtgct
     3241 cgcagtcaca atcgtcatgt cgttttcgag cacgaccagg ggatcgcgga tcgtgtcgac
     3301 gatactttga gtcagttgcc agttgcgctt gctctcagcc cgggccgcga gttcctcgct
     3361 gacacgatgg ttgacctcgc gcaacagcag agcccgttcg ttcgcgagcg cttcgaaccg
     3421 atcccgcgca gcacgcacct ctgcttcggc agcttccttg gcacgacgta acattgctgc
     3481 atcgagggct gcatcaatgg ctctctgcaa aagcgccagg aactcgcctt gtacgtcctt
     3541 gacgacgtaa tccgccgcac cggccttcag ggccgcgacc gccactcggc cttcctgcat
     3601 cgcggtcaca tagatcaccg gtggcggttc cgccaagttg cgaatgctcg ccaaggtctc
     3661 caggccgtcc tggttcggca tgtaatggtc gagggcgacg acgtcgatgc cgccctgtgc
     3721 tatccgggca acgccgcttg caccgtcggt cgcgatctcg acgacataac cctgacgttc
     3781 caaatccttc tggacgagcc ggcacaggcc aggatcgtca tcgatgtaga gaatgcgagt
     3841 gcggagtgcg ggcattagat cgtctcggga acctgcatga cgtacacaaa taaccctagc
     3901 tgctggatcg cattggcgaa attctcatag ttgaccggct tggtgatata gacgttgcag
     3961 ccgaggtcgt agcagcgttg gatttcggac ttatcatcgg tcgttgtcag taccaccacg
     4021 ggtgacactc tgagatgttc gttctccttg aggcgtttca agatatcaac gccggacata
     4081 tccggtaaat tgaggtccag gaggatcagc aaagcgcggc ctttgttgac aagaccggag
     4141 ccatcagaac cgaacagaaa cgtaatcgca ctcgcaccgt ccgcaagccg cacgatctca
     4201 ttgtggactc ccgctcgacg gaggttcttc tcgatgaggc gagcgtggcc ctcatcgtct
     4261 tcgatcatcg cgattgtaac tggttgggtc atgtcatcat ctcacttact ggcaaaggcc
     4321 acctcttggg caacgtcact ttgaatacgg tgccgcggtc aggctcactc tcgacgttga
     4381 tcgtgccccc gaggcgccgc acaagcgcac gcgcatgagc gaggccgatg ccatcgcccg
     4441 gccggtcctg aatgcccgat cgtcggaaca gatcaaagat acgctcacga tcctttgcat
     4501 cgatgccgcg accgttgtcg tgaacctcgt aggtgatcgc tccatcagat tcggttgctc
     4561 ggacctcgat gctcccaggc acgccggccc gcaaatactt gaccgcgttg tccatgaggt
     4621 tcgagaacac ctgttcgagg gcgagacggt cgctggtgat cgtcggaagt tctccaatga
     4681 tgattttggc gttaacgtcc tgcgcttgat gagccattgc ctccaaagca gatcgcaaca
     4741 actgcgacat atcgatgaat tgcggctgaa actgacgctg cccctcacgc gacagcttga
     4801 gaagggcgcc gatgagtcga tccattttcg caatcgatga tttgatgaag ctgagtgctt
     4861 cttcgacgtc actgcgcagc gcctcatcgc tttcactatc ctgctcgccc ccgacttgag
     4921 cacgtagcgc ccctatccgc tcaaagatct gcttccgcca agcttcgagt tcgctggcga
     4981 aacccatgat attgacgaga ggcgagcgca agtcgtggct gacgatgtat gcaaaccgtt
     5041 gaacctcctc attcgcctcg ctcagaagag cagtacgttt tgccacgatc gtctcgagat
     5101 tctggttggt cgcctcaagc atacggttag catcttgctg ctccttgagc gagtgacgga
     5161 gcagcagcac cgaggttgcc gcaagcacaa gaatgagtat ggagcctgtg agcgtgacga
     5221 tcaaaagcca gcggctcgaa ttgtccaatg ccgccgtcct tacgttttgc aatcgttgtt
     5281 cctcattctc catgcggccg atcaccgtta aaatctcctg catgagagtc agcccagtcg
     5341 cgccgaccag cgccgacgca cccgctgcgt caccagactg gtaaagacgg accgtctcgc
     5401 gcagttcatc gagcttcgcg ttgaccgctg gccggagccc agcgaggaaa tcttgctgcc
     5461 gcgggttgtc gcttgttgta ctggataatt cgtcaagggc cggcgcgatc gccttcatgt
     5521 catcttggta ggttgccaaa tagcccagat cgcttgtcag caaatatcca cgctggctgc
     5581 tctcggaacg ccgcaataga agccgaagat tcgccagttt gtttcggact tccagggtgt
     5641 gtgcgacttg gtcggcgccg aattcgactt gcttcacgaa ccaaatagaa atggcgctaa
     5701 tcgtgataag gataacgaac cccagagcca gaaaaatagg ctcacgccga accaagcgac
     5761 tcactgtcgg gggggtcatc gtgtcccgtg ccggcgaaag tgctcagcca ccagccctgc
     5821 caatttagca tgatcgaagg gcttcaccag ccgcggctcc gtccggaaat caatcggcat
     5881 catagcggca ttgccgtagt aggtcagacc ctcagtctca tcggggctcg tgcggaacgt
     5941 ctggtagaac gcgcgatcgg cagatgtgat gtttaaattt tgatcaagaa cgatgagtgc
     6001 gctgggcgcc gtcgcaacaa tgtcgctcgc agctatgtcg gataacggca ttctcccccc
     6061 atcaattccg cgacttaaaa attacgctgc gagcaggttt tctacagagt tttggagctg
     6121 atattgtgtg tacggcttac aaagaaagtg tgagccttta acaaacagag ctttcatttt
     6181 gtcggtgatg gaattgccgg tcgtgtagag cacacgcaaa ttccgccgaa gcttgatggc
     6241 ctggggtgca agatcacacc cgccaaggac cgccgttttg agatagatat cggtaaaaag
     6301 tgcgtcgatg tgttgaggag agcgcagcag taaaagcgct tcgtccacgt cgctggccga
     6361 aagcgtttta tgaccccagt cttggatcat catctcagcg atttcgcgga taaacacatc
     6421 gtcttcgacg ataaggatca cggccattac aactctttcc ggccgtgaca accagcattg
     6481 cggcgagcag cactcttcct gccggtatca tagcaatcag agcccgttcg accgctatca
     6541 gggcataggg atactcccaa ccggagctta cggttccgca aagttttaaa ttcttttaca
     6601 taaaacggct ttatggaagt tatcgcgtta tatcaaagac ttatctggac ctctggtgcg
     6661 tacctccacg ccggcgggcg tcttgccctc gcgcaggatc accgccacgg gcttgccgct
     6721 ggtgttttaa tagatgtgga tcggcaggaa gcagcgctcg tcgtaatgcg cgttgaacaa
     6781 cgacaattgc tgatggccgt gcatggcatc gaaggtgtca tcgatgtcga gggtgatcga
     6841 ggccggcgcg ctgcggtagc tctggccgcg aactggtcca ccatggccgc catcgcccgc
     6901 gcgatctcga tcttcgaggg ggcattctcc agccgcgaca tcgtcggctg cgagcacaga
     6961 ggatctggtg agcaacagtg acgtaacggc cgttatcgcc atggacggcg tagacgccga
     7021 gtgtcacgcc atagccttgc cgtcgcggcc gggccaggcg gcgccgattt cgagccagga
     7081 gccattcgct ccgcctgcct tcttgacgat ggctgcgtaa acgctatgcg tcgtgcggtt
     7141 ggagctggtt tcagtggtgt gtgtaccgaa aacctcatcc gccgatgaat tcggtgcccc
     7201 cgctggtcgc cgcctggctt ggcgctattt ctgcagattg ggggactcgg ccattccaag
     7261 acctaggaag agacggcgag aaacggactg tcagaacctc cgcttgtttt gaccggggtt
     7321 tgtccactcg tctggcatgg ttgtcagaac gaccctgagc aatatcaagg gtttaaatca
     7381 ggttcaaatc ctgccactcc gaccagctaa caaattgatt ttgcacgctt tcccgaaaag
     7441 cgccagtggc cgaattttcc accgccacta aaaccgccac tgaaacgact tggccgacgc
     7501 cgttccgcca gcttaaggg
//
LOCUS       DATMGX010000034         3173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_472087, whole genome shotgun sequence.
ACCESSION   DATMGX010000034 DATMGX010000000
VERSION     DATMGX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472087"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..924
                     /locus_tag="VNR65_00575"
     CDS             391..924
                     /locus_tag="VNR65_00575"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWJ17197.1"
                     /translation="MVAPALRVTLAIALCAAFATPAAPRGLLDFFKSFLTASVLPAHT
                     VKQARTKTKQGYGTKVHGKVKINDKSFDFVSGGRGRGSTPFGTYKIGPLSGFKTPKGA
                     WIPGYQLSDAYDPFVKDTRTGLFIHPGHNDSAGCVAIRKDEWPSFVKAMEGAAQVGRP
                     LAIHLGPETEPMQKPVG"
     gene            complement(1056..2648)
                     /locus_tag="VNR65_00580"
     CDS             complement(1056..2648)
                     /locus_tag="VNR65_00580"
                     /inference="COORDINATES: protein motif:HMM:NF009120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWJ17198.1"
                     /translation="MGLTSRAALLCTAGFTAVALGGTSSANATGTRQGERSTQSRAAG
                     APLLAIIALAQQRVTVYGASGKMMEGPVSTGATGHETPAGIYSILEKEEEHHSNLYDD
                     ASMPFMQRLTWTGISMHAGVLPGYPASHGCTRLSYGFAQKFYQVTKPGMRVVIVREDI
                     IPAEIEQPELFNRPAPEVSPSGINATDSPSEADIHTRLAAIKEAKLAKAEAAKKRELE
                     AQSAAANKAAQAASAAHLAEAAAANLARMETALDAGERMRETVFNRTGNAEPTRTAAA
                     AKIQAARLQLERAKTEAQAKKDAAVEAEEEAKLAAAMSDEAAEAAETARENAWPISIF
                     ISRQAQRLYIRRGREPVFEGPVTIRNPDEPIGTFVFTALSYTGTPGRLGWNVVSMYKN
                     ATAIEPYSDAKREPKQRREPKPADVAGARNAMQRLVIPQAALDHISGTLLTGSSLIIS
                     DEGTSSETGKDTDFIVFMSGEPKGGAAFRSPVIADAAGEGRRAHGQTRRTSSSSSRSR
                     HQAAGAARRGREFSPFSSLFGF"
BASE COUNT          672 a          891 c          881 g          729 t
ORIGIN      
        1 taaacttgcg tgaaactaag ttgcgcgaga cgtgaagcac gaaccgggaa taccgacgac
       61 agtggatttc gggggcccgt tcgagaaaat gccaagcttg tgcatcatgc catggtgtag
      121 ttaatcctat ttacaactga gcatagagaa caatcgcttt gattttacga aaatttattt
      181 taaataaaag cctataagat gaacattatt taactagtga agagtgaacg gcacgtatag
      241 ctaagcgcga aatagcttca cctctaggct atcgtcctac acctcgcacg tcgtgtttat
      301 gacgatatcg gtgattagcg cggcttcggc tcctcacgtt gggccctctt catgccaaga
      361 ctacgacttc gagagaaagg agccaatcct ttggttgcac ccgccttgcg tgtcacttta
      421 gctatcgctt tgtgcgcagc cttcgcgaca ccagccgctc cgcgagggct tctggacttt
      481 tttaaatcct ttttaaccgc ttccgtttta cccgctcaca cagtcaagca agctcgaaca
      541 aagacaaagc agggctacgg taccaaggtg cacggcaaag ttaagatcaa cgacaaaagt
      601 tttgattttg tttcaggtgg acgaggtcgc ggctcaacgc cgttcggcac ctataaaatt
      661 ggaccgctta gcggcttcaa gactccaaaa ggagcctgga ttccaggtta tcaattgagc
      721 gatgcctatg atccttttgt caaggacacg cggacaggct tattcattca tcccggacat
      781 aacgactccg ccggctgtgt agcgatacgg aaggatgaat ggccttcgtt cgttaaggcc
      841 atggaaggag ctgcccaggt tggccgccct ctagcgatcc accttggtcc ggaaaccgaa
      901 cctatgcaga aaccggtagg ctgagggact tcactacgca tcattcttca ccgcatggcg
      961 ggcggttcac gtgttgaccg ctggtttata tgttccaagt atctacttta ttttcggccg
     1021 ccggatcgca aggagagtgc gcccggacac cggattcaga agccaaagag tgaggagaac
     1081 ggagaaaatt cccggcctcg cctagccgca ccggcagctt ggtgcctgga ccgggaggac
     1141 gacgagctgg ttctccttgt ctgcccgtgt gcccgccgtc cttcgccggc cgcatccgca
     1201 atgaccgggc ttcggaaagc ggcgccgccc ttcggttcgc cgctcatgaa tacgatgaaa
     1261 tcggtatcct ttccggtctc gctgcttgtt ccttcatccg agatgatcag cgaggacccc
     1321 gtcagcagcg tcccggagat atggtcgagc gctgcctgcg ggatgacaag ccgttgcatc
     1381 gcgttccggg ccccagcgac gtccgccggc ttcggctcac gacgctgctt cggttcgcgc
     1441 ttcgcgtcgg agtaaggctc gatggcggtc gcgttcttat acatggacac gacattccaa
     1501 cccaggcgcc cgggagttcc agtgtaactg agggcggtga aaacaaaggt gccgatcggt
     1561 tcatctgggt tgcgaatagt tactgggcct tcgaacacag gttctctgcc tctccggata
     1621 tagagacgct gtgcctggcg gctaatgaag atcgaaatgg gccaagcgtt ctctctggct
     1681 gtttctgcag cctcggcagc ctcgtccgac atagcggcag cgagcttcgc ctcttcttcg
     1741 gcttctaccg ctgcgtcttt tttagcctgt gcttcggtct tggccctctc aagctgtaac
     1801 cgcgccgcct gaatcttagc cgccgccgct gtcctggtgg gttcggcatt tcctgttcga
     1861 ttgaaaacag tctcgcgcat gcgttcgcca gcatccagtg ccgtttccat gcgagcgaga
     1921 ttggcagccg ctgcctcggc caaatgggcc gctgatgcag cctgcgcagc tttatttgcg
     1981 gccgcagact gcgcttccaa ctcgcgcttc tttgcggcct ccgctttcgc cagctttgct
     2041 tccttaatgg ccgcgaggcg tgtgtgtatg tcggcttcgc ttggcgagtc ggttgcgttg
     2101 atgccggacg gcgaaacctc cggtgctggg cgattaaaca gttcgggttg ctcgatctcc
     2161 gccggaatta tgtcctcgcg aacgatgacg acgcgcatgc cgggtttggt cacctgataa
     2221 aacttctgcg cgaagccgta agacaggcgc gtgcagccat gagacgccgg ataacctggc
     2281 aaaacacccg catgcataga gatgccggtc caggttaagc gctgcatgaa cggcatggat
     2341 gcatcgtcat aaagattgga gtggtgttcc tcttccttct ccagaatgct gtaaatccca
     2401 gccggtgtct cgtggcccgt agcgcctgtc gaaactggcc cctccatcat ttttcccgag
     2461 gcgccgtaga cagtgacccg ctgctgcgcg agcgctataa tggccaagag gggcgcaccg
     2521 gcggcacgag attgagtgga gcgctcgcct tgccgggtcc cggtcgcatt ggcggaggaa
     2581 gtcccaccca aagcaaccgc ggtgaaacca gctgtgcaaa gcagggccgc tcggctcgtc
     2641 aatcccattg ctggcctcag cttccataaa ccctttaaga atttgcttca aataagcgtc
     2701 tacttcgaaa atggttccaa ccacagccgg tcacacttga ctatgccgaa ggtgcacgct
     2761 tgacgtcagg cgcgggaagg cgaagttgca gacgcataag ccaacaaagt tggcgtccgc
     2821 cataccttca gttgcgtcgt atggttcgct aatttcgaac ggcggctccc ggcctcttgc
     2881 gttggtgcgt cggaagatcg attagcgggc tcatgcgcgc gaccggaggg gcagaggcaa
     2941 acggcagtcc tcgtccgcgc ggagcaagcc tgattagtcg aaaaactcga atgatgatgc
     3001 cgcgaacatc cctcagagca attatggtgc ctatcggcgg ggcggaacaa agttgctcgg
     3061 acaagtatcc cccgctgttt gcggcctaga ggtcgatgcg aaatcaaata ggcacaagga
     3121 ttttacttag aatcctcaaa atacctgtgc tattctatcg gaagatgaat tta
//
LOCUS       DATMGX010000035         2049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_285099, whole genome shotgun sequence.
ACCESSION   DATMGX010000035 DATMGX010000000
VERSION     DATMGX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285099"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VNR65_00585"
     CDS             complement(<1..760)
                     /locus_tag="VNR65_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ17199.1"
                     /translation="MEPTELLIGPGLTGVVGPNGCGKSNLLEALRWVMGETSYKSMRA
                     SAMDDVIFAGTDRRPARNMAEVMVAIDNSQRTAPAAFNDADVLEISRRIQRDAGSIYK
                     VNGKEVRAKDVQILFADAATGARSQALVQQGQIGQLINAKPQERRRILEDAAGIAGLY
                     SRRHEAELRLKAAEANLERLKDVMGQIGSQLANLRRQARQAQKYKELTAELRKLEAIQ
                     HHQHYATANATVQAEEAQLLEALRAVGQFTQAESS"
     gene            complement(1077..1769)
                     /locus_tag="VNR65_00590"
     CDS             complement(1077..1769)
                     /locus_tag="VNR65_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWJ17200.1"
                     /translation="MYRNRKIAVFSPGAIALAAVLAFSPFAITGGRIGLEAAIAADDQ
                     AAPAKEAAPAPAESALPDMVLGKEDAPVTIVEYSSLSCPHCAVFHKDVLPGLKSEYID
                     TGKVRYIEREFPLNNPALAGSVIARCIDSSRFFAFNDLLFSKQEDWAFKDDAFKPLRL
                     YAKQAGLTDADFEKCIDDEALQKKILAVRERGEKDGVHGTPTFFINGKIFSGTPTLQA
                     LAEAMKPYLSTQ"
     gene            complement(1854..>2049)
                     /locus_tag="VNR65_00595"
     CDS             complement(1854..>2049)
                     /locus_tag="VNR65_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17201.1"
                     /translation="FRAVSGLRVVQAPILRKEATPRPVKPVPAEVKPALAEDELKPEN
                     RLSAALARMARGVKARGCSG"
BASE COUNT          397 a          625 c          603 g          424 t
ORIGIN      
        1 cagacgattc ggcctgggtg aactgcccga ccgcgcgcag cgcctcaaga agttgcgcct
       61 cctcggcctg cacggtggca ttggccgttg cgtagtgctg gtgatgctgg atcgcttcga
      121 gcttgcgaag ctcggccgtc agctccttgt atttctgcgc ctgacgggcc tgccgccgca
      181 aatttgcgag ctgcgagccg atctggccca tcacgtcctt cagtcgctcg agattggctt
      241 cggcagcctt gaggcgcaac tcggcctcat ggcggcggct gtagagcccc gcgatgcccg
      301 cagcgtcttc taggatacga cggcgctcct gcggctttgc gttgatgagc tgcccgatct
      361 ggccttgctg gacgagcgcc tggcttcgcg cgccggtagc tgcgtcggcg aagaggatct
      421 gcacgtcctt ggcgcgcact tccttgccgt tcaccttgta gatggagccg gcgtcgcgct
      481 ggatgcgccg ggagatttcc aagacatcgg catcgttgaa agcggcaggt gcggtgcgct
      541 gcgagttgtc gatggcgacc atcacctcgg ccatgttgcg ggccggacgt ctgtcggtgc
      601 ctgcgaagat gacgtcgtcc atcgctgacg cgcgcatgct tttataagac gtctcgccca
      661 tcacccagcg cagcgcttcg agaagattgg attttccgca gccgttcgga ccaacgacgc
      721 cggtgaggcc ggggccaatc aaaagctcag tcggctccac aaaggatttg aagccaagaa
      781 gcctcagccg tgtgatttgc aaaagttccg cccttccgtt cgaccgcccg cgttaagccg
      841 gccattgacc gtccgcggat gcgcgtcagg ctgatctcct caccagcgga tcaacaatga
      901 tttgattcgc aaggcgcgcg gcacgtttgc cgcactcaaa gccctcgaat tcaacaacat
      961 tatacgcaag aaaaaactgc cgatgcgcct ggtggttctc acccctatcg aaaaacgcaa
     1021 gcggcaacta aaaagcgttg cggcggcaaa gccgccgagc cgggctattc tgatcgctac
     1081 tgcgtgctca aatacggctt catggcttcg gccagcgcct gtagtgtcgg ggtgccgcta
     1141 aagatcttgc cgttgataaa aaacgtgggc gttccatgca ctccatcttt ttcaccgcgt
     1201 tcacgcacgg caagaatctt cttttgaagc gcttcgtcgt cgatgcactt ctcaaaatcc
     1261 gcgtccgtta gcccagcttg ctttgcgtac aaccgcaggg gcttgaaagc gtcatccttg
     1321 aaggcccaat cctcttgctt cgaaaacaac agatcgttga aagcgaagaa tcggcttgag
     1381 tcgatgcagc gcgcgatcac agatcccgcc agcgccggat tgttgagagg gaattcacgc
     1441 tcgatgtagc gcactttgcc ggtatcgatg tactcggact tcaaacctgg aagcacatcc
     1501 ttatggaaga cggcgcagtg agggcagctc agggaagagt actcgacaat ggtgacagga
     1561 gcgtcttcct tgccaagcac catatcgggc agcgcgctct ccgcgggagc aggtgccgcc
     1621 tctttcgccg gcgcagcctg atcgtccgca gcgatcgccg cctcaagacc gatgcgaccc
     1681 ccggtgatcg cgaatgggct gaaggccaaa acggcagcta acgctatcgc ccctggcgaa
     1741 aaaacggcaa tctttcggtt cctgtacaca ggcgtctcca tttagatcca tttcgtttgt
     1801 tttggagcgg cgaaaagcaa atttcaagtc ggcgcagcgc ggcggcgggg atcttagccc
     1861 gaacatccac gggcttttac gccacgcgcc atgcgcgcca gggcagcact caggcgattt
     1921 tcgggtttga gctcatcttc cgcgagcgcg ggcttcactt ccgcggggac gggctttacc
     1981 ggccggggtg ttgcctcctt ccggagaatg ggagcttgca caacccgcaa accggacacc
     2041 gcgcggaac
//
LOCUS       DATMGX010000036         2714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_405311, whole genome shotgun sequence.
ACCESSION   DATMGX010000036 DATMGX010000000
VERSION     DATMGX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405311"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VNR65_00600"
     CDS             complement(<1..272)
                     /locus_tag="VNR65_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17202.1"
                     /translation="MREKVNVIISSTVAGAIQDRLHSLQGLTSRLLDLSLMQINGVAA
                     QFRAIGRNVYRFTLFAPRPMMLDARKNWSAVDGCRYILIAMSNKLP"
     gene            complement(322..996)
                     /locus_tag="VNR65_00605"
     CDS             complement(322..996)
                     /locus_tag="VNR65_00605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWJ17203.1"
                     /translation="MRNALSQSEMRLRQKDELIQRLELLKKESDHRLLNDLQMTISLL
                     SLQSRGSTNAEAAEQLAVAANRVAMIARIHHRLNSNEGVQTIEFKKFLEDFARDFSTM
                     VSLEDSPAQIIVDGNEINLPAATAIPLGFIASELLTNAAKYGSGRIAVGLEPNPELGY
                     ALLVANDGPALPNDFDPAASKGLGMRIVQSFARQIGGELRSGRGDDGQGARFAVLFSA
                     PMGASD"
     gene            1296..1970
                     /locus_tag="VNR65_00610"
     CDS             1296..1970
                     /locus_tag="VNR65_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWJ17204.1"
                     /translation="MEKATLDEFDPRAFLEKVGEGKTVSTFHKNETVFSQGDAADTVF
                     YIQKGRVKVVVLSEQGKEAVVGILEAGQFFGEDCLNGNPLRISTTTALEDCVITAITK
                     TAMLAVLHSEPKFSELFMSYLLTRNSRIEADLVDQLFNSSEKRLARLLLLLANFGKEG
                     SPQPIKVQVSQETLAEMIGTTRSRVSFFMNKFRKLGFISYNGKIEVHNSLLNAVLHDT
                     PQLRRD"
     gene            2092..>2714
                     /locus_tag="VNR65_00615"
     CDS             2092..>2714
                     /locus_tag="VNR65_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWJ17205.1"
                     /translation="MLGALEPSSRERIDHHLEPVKLKLGAVVCDAGGLLKHAYFPEGS
                     VLSLLTVLENGSAIETANIGREGAFGLFAAMYSRVSFNRCLVQLEGGMVRCPIELLQS
                     EFRNSEHVRNLFVSYSETLLSQVQQTVACNALHSTEERMCRWLLMMHDRAEGEALPYT
                     HEFLSEMLGANRKSVTMAAQALQTAGLISYRRGKIQVVDRAGLEKAS"
BASE COUNT          612 a          746 c          763 g          593 t
ORIGIN      
        1 ctcggcaatt tgttactcat cgcaattaaa atatagcggc atccatcgac agcggaccaa
       61 tttttgcgcg catccaacat cattggacga ggggcaaaca aggtgaacct gtagacgttc
      121 cttcctatcg cgcgaaattg agccgccaca ccattgatct gcatcaaaga caaatccaac
      181 aatcgagagg tgaggccttg cagcgagtgt aggcgatcct gaatagctcc agctaccgtc
      241 gagctgataa tgacattgac tttctcgcgc aagatggtgt gcgcggcgtg cgcctacaga
      301 tcgaccaggt ggcgtaggcg tctaatcgct tgctcccatt ggtgcagaaa atagcacggc
      361 aaatcgcgcg ccttgtccgt catcgcctcg gccgctccgc agttcaccgc caatttgcct
      421 tgcgaaagat tgaacaatcc gcatcccgag tcccttgctc gcggcggggt cgaaatcgtt
      481 cggcaaggcc ggcccatcat tggcgaccag aagcgcgtat cctagttccg gatttggctc
      541 cagcccgacg gcgatccggc cgctgccgta tttggccgca tttgtgagta gttcgctggc
      601 gataaagcca agcggaatcg cggtggcggc cggcaaatta atttcattgc catcgacgat
      661 aatctgcgct ggcgaatctt ctaaggacac catcgtcgaa aaatcgcgcg caaagtcctc
      721 aagaaatttc ttgaattcta tagtttgcac accctcgttg gaattaagac gatggtggat
      781 tcgcgcaatc atggctacgc gattagccgc aacggccaat tgttcggccg cttctgcatt
      841 tgtcgatccc cggctctgca acgaaagcag gctgatggtc atctgcaggt cattcaacag
      901 tcgatgatcg gactcctttt tcaatagttc cagtctttga attaattcgt ctttttgtcg
      961 aagccgcatc tcgctttgcg aaagagcatt tcgcaattga atctccgtga gcctgtcatc
     1021 cgttggctcc aaattgtgca ggcatcgatc aaggttctga aaaattacgc ccggaggcgg
     1081 gtccgaggcg caggaagagc gttctgtgat catatgtcgt ccccatgagt gtctcgcact
     1141 cgctgccttc aaacgcacca ttgaaagaaa gcctagggtc gaaaaagcag atcagattga
     1201 tgaaatcaac tatgtccgat atccgacatt atatttgcat atgagaattg ctggatatgg
     1261 cccctcagac aattttagtt gagatggagc gaactttgga aaaggccacg cttgacgagt
     1321 ttgatcctcg cgccttcctc gagaaggtcg gtgaaggaaa gacagtatcg acattccata
     1381 agaacgagac cgtgttctcg cagggagacg cggcggatac tgtcttctac atccagaaag
     1441 gcagggttaa agttgtcgtg ctatccgagc agggcaagga ggcggtcgtc gggattctcg
     1501 aggccgggca gttcttcggc gaagactgct tgaacggcaa ccccctgcgg atatccacga
     1561 ccacggcgct ggaagattgc gtgattactg ccatcacgaa gaccgcgatg cttgcggtcc
     1621 ttcacagcga gccgaagttc tcggagctct tcatgtcgta tctgctgacg cgcaatagcc
     1681 gaattgaagc ggacctggtc gatcaactct tcaattcgag cgagaagcgg ctggcgcggc
     1741 ttctgctgct gttggcgaac tttggcaagg agggcagtcc gcagccaatc aaggttcagg
     1801 tcagccagga aacattggcg gagatgatcg gcaccacccg ttcccgtgtc agcttcttca
     1861 tgaacaagtt ccgcaagctc ggcttcatca gctacaacgg caagatcgag gttcataact
     1921 cgcttttgaa tgccgtcctt cacgacacgc cgcagcttcg aagggactag ccgggcggcg
     1981 gccatgtggt cgattttaag ggggtgtttg ctgcccgcgc aggggtaacg caaaacagac
     2041 agatcggacc gaatgccgaa acttcgaata gttgagccac aaggcaaccg attgctgggc
     2101 gcgctggagc catccagccg cgaacgcatc gaccaccatc tcgagccggt caagctcaaa
     2161 ctgggcgccg tggtctgcga tgcgggtggc ctgctcaagc acgcctactt tccggaaggc
     2221 tccgttctgt cgcttttgac ggtgctggaa aacggttcgg cgatcgagac cgcgaacatc
     2281 gggcgcgagg gcgcattcgg cctgttcgcg gccatgtaca gccgggtctc cttcaaccga
     2341 tgtctggtgc aacttgaagg cggtatggtc cgctgcccga tcgaacttct gcaatcggag
     2401 ttcaggaaca gcgagcacgt ccgcaacctg ttcgtcagtt actcggaaac gctgctgtcg
     2461 caggtccagc agaccgtcgc gtgcaacgcc ctgcacagca cggaagagcg gatgtgccgc
     2521 tggctcttga tgatgcatga ccgcgccgag ggcgaggcct tgccctatac ccacgaattc
     2581 ctgtcagaga tgctcggcgc caatcgcaag tcagtgacca tggcggcgca ggcgctccag
     2641 acggcggggc ttatcagcta ccggcgaggc aagatccagg tcgtcgatcg tgccgggctc
     2701 gaaaaggcgt cttg
//
LOCUS       DATMGX010000037         4496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_22484, whole genome shotgun sequence.
ACCESSION   DATMGX010000037 DATMGX010000000
VERSION     DATMGX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22484"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..843
                     /locus_tag="VNR65_00620"
     CDS             118..843
                     /locus_tag="VNR65_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWJ17206.1"
                     /translation="MQKEVEKTEHLGKREQLGAHMVNVYINGKRHIVPEHATIMRAIE
                     YAGHQIIRGAGCREGFCGACGTIYRLPGDYRLHTGLACTTLVKEGMALTQIPSVPMEK
                     AVYDLEKLTPDVNAILQVYPVTFRCVACGTCNKACPQNLPVMDYIQSAMRGDIAAVAD
                     MSFDCVACGLCALRCPAEIIQHEVGILSKRLTGRYLRKESKELDDRLEEINSGKYNSE
                     YVKLMGLDKVSLSELYYARDVEP"
     gene            925..2652
                     /locus_tag="VNR65_00625"
     CDS             925..2652
                     /locus_tag="VNR65_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWJ17207.1"
                     /translation="MYPEYLSGQVKLVEKTRAKRVDLALSGKPVYPPMNAEEREDVLS
                     KYHPDYAPGAKRAVRVGPNRGQELTTEVATILEAHSMIRPKLTSTLLTEPDYETDLLI
                     IGGGGAGCWAAITAARKGMKSLIATKLRMGDANTMMSEGGMQAAVSRTDTPTRHYLDA
                     MGGGHFDNDPALVRVLTEDAPEVVRELENLGVIWDKMPDGSMQVLHGGGTSRKRMVSC
                     RDYIGAIIMRTLMDEVRNHPDMISVREFEPAVELLLDEHGKCAGAILYSLDTEQFLTV
                     KAKSTIIATGGFGRLHIRGFATTNHYGATGDGLVIAYRAGAKLKYMDSVQYHPTGAVF
                     PEQIAGFLITEKIRGAGGQPLNKYGELFVFPREPRDVESAAIIRECSPDRDNGIDTWV
                     GRTGVWLDSPIIEEIHGEGYIRKQFPGMCRQFDRFGIDIVKEPMLIYPSLHYQNGGVA
                     INERCETSIPGLYVAGEASGGVHGRNRFMGNSVLDYNVFGRRAGQHATEYAQTVTLGR
                     LSLEHVAAHEREMEYAGIETDLTSPLILPSYTPEDVKNHQLARLGNVPALHSMLRSRF
                     VPGERRTRP"
     gene            2672..3085
                     /locus_tag="VNR65_00630"
     CDS             2672..3085
                     /locus_tag="VNR65_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWJ17208.1"
                     /translation="MSVKINGNGAAANAFIEKVESLSKQDLLACYQCGKCSAGCPMAK
                     YMDILPHQIIRFAQLGLKDELLASEAIWMCVSCLTCNTRCPKGVRIAEVIEAVRQVRL
                     RKRRNDHLIVRELPASALATLPPIAIIGSMRKGTS"
     gene            complement(3178..>4496)
                     /locus_tag="VNR65_00635"
     CDS             complement(3178..>4496)
                     /locus_tag="VNR65_00635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HWJ17209.1"
                     /translation="ARGEDAAADNRPLIQEILALRGELATLLGFETYADYQIDDAMAK
                     TPASARELLERVWDGAKLRADEEREALQSMAQAAGHNEPLAAWDWHHYTEKRRKAEFD
                     IDGAALKPYFELNNMIAAAFDTAHRLFGLGFNERGDMPVYHPDVRVWEVLNREGKVIA
                     LFMGDYFHRPSKRSGAWMSAFRRQERLDGEVVPIIVNVLNFTKGGQGTPTLLSLDDAR
                     TLFHEFGHALHGLLSDVTYGSLAGTSVDRDFVELPSQLFENWLLKPEVLNRFARHGET
                     GDPIPQSLVEKLHAARKFNQGFATAEYTASALFDLDIHEVASPGALDAGEFEQDMRAR
                     IGMPDAISLRHRPPHFQHIFASSGYAAGYYTYLWAEVMEADAFEAFEEAGDIFDPAVA
                     ERLRRFVYSSGGTMKPEEAYRAFRGRDPQVEPLMEKRGLLQAEEID"
BASE COUNT          935 a         1425 c         1331 g          805 t
ORIGIN      
        1 cgacgtctac cgctacatga acttcgacca gatcgacgaa tatcgaagcg ccgcaacgaa
       61 aacagcggcc tgacacaaga gctggcgctt cgcgcgaagc cctgatgagg aacggaaatg
      121 caaaaggaag ttgagaaaac cgaacacctc ggcaagcgcg aacagctcgg cgcccacatg
      181 gtgaacgtgt acatcaacgg caagcgccat attgtgcctg agcacgcgac catcatgcgg
      241 gccatcgaat atgcgggcca tcagatcatt cgcggtgccg gctgccgcga aggcttctgc
      301 ggcgcttgcg gcaccatcta tcgccttccc ggggattaca ggctccatac agggctcgcc
      361 tgcacgacac tcgtgaagga gggcatggcg ctcacgcaga tcccgtccgt gccgatggag
      421 aaggcggtct acgatctcga aaagctgacg ccggacgtca acgcgatcct gcaggtctat
      481 ccggttacct tccgctgcgt cgcctgcggc acctgcaaca aggcctgtcc gcaaaatttg
      541 cctgtgatgg actacatcca gtcggccatg cgcggcgaca tcgcggcggt cgctgacatg
      601 tcgttcgact gcgtcgcctg cggtttgtgc gcgctgcgct gtccggcaga gatcatccag
      661 cacgaggtcg gcatcctctc caagcgcctc accggccgct atcttcgcaa ggagagcaag
      721 gagctggacg accggctcga ggagatcaac tcgggtaagt ataactccga atatgtgaag
      781 ctgatgggct tggataaggt gtctttgagc gagctttatt acgcccgcga cgtcgagccc
      841 tgagccaggg catgaacggc atgagacacc aattcaagcg gtaattacgc gggcggcccg
      901 caccgcccct tctgggagtg agacatgtat cctgaatatc tatccggaca ggtgaagctg
      961 gtcgagaaaa cccgcgcgaa gcgggttgat ctggcgctca gcgggaagcc cgtctatccg
     1021 ccgatgaacg cggaagagcg ggaggatgtc ctctctaaat accaccccga ctatgcgccg
     1081 ggagccaagc gcgccgtccg cgtcggcccg aacaggggcc aggagctgac caccgaggtg
     1141 gccaccatcc tggaagcgca ttccatgatc cgcccgaagc tgacaagcac gcttctgacc
     1201 gagcccgatt atgagactga cctcctcatt atcggcggag gcggcgccgg atgctgggct
     1261 gccatcacag ccgcgcgcaa aggcatgaag tcgcttatcg ccaccaagct gcgcatgggc
     1321 gacgccaaca ccatgatgtc ggaaggcgga atgcaggccg cggtttcgcg cacggatacg
     1381 ccgacgcgcc attatctcga cgccatgggc ggcggccatt tcgacaacga tccggcgctc
     1441 gtgcgcgttc tgaccgagga cgcccccgaa gtggttcgcg aactcgaaaa tctcggcgtc
     1501 atttgggaca agatgcccga tggcagcatg caggtgctgc atggcggcgg cacctcgcgc
     1561 aagcgcatgg tttcctgccg cgactatata ggcgcaatca tcatgcggac cctcatggac
     1621 gaggtgcgca accatccgga catgatttcc gtccgcgagt tcgaaccggc ggtggaactg
     1681 cttctcgatg agcatggcaa atgtgcgggc gcgatcctgt acagcctcga cacggagcag
     1741 ttcctgacgg tgaaggcgaa gtccaccatc atcgcgacgg gcggcttcgg ccggctgcac
     1801 attcgcggct tcgccacgac aaaccattac ggcgccactg gcgacggcct cgtcatcgcc
     1861 tatcgcgcgg gcgccaaatt gaagtacatg gactcggtgc agtatcaccc cacaggcgca
     1921 gtgttccccg agcagatcgc gggatttctc attacggaga agatacgcgg cgcgggcggg
     1981 cagccgctca acaaatatgg cgaactgttc gtcttccccc gcgagccgcg cgacgtcgaa
     2041 agcgccgcca tcatccgcga atgcagcccc gaccgcgata acgggatcga cacctgggtc
     2101 ggccggacgg gcgtttggct ggattctccc atcatcgagg aaatccatgg cgaaggctac
     2161 atccgcaagc aattccccgg catgtgccgc cagttcgacc gcttcggcat cgacatcgtg
     2221 aaggagccga tgctgatcta tccatcgctg cactatcaga atggcggcgt cgccatcaac
     2281 gagcgctgcg aaaccagcat cccgggcctt tatgtggcgg gcgaggcatc cggcggcgta
     2341 cacgggcgca accgcttcat gggcaactcg gtgctcgact acaacgtgtt tggccggcgc
     2401 gccgggcaac acgccaccga atatgcgcag actgtgacgc tcgggcggct cagcctggag
     2461 cacgtcgccg cccacgagcg tgaaatggag tacgccggca tcgagaccga cctcacatcg
     2521 ccgctgatcc tgccttccta caccccggaa gacgtgaaga accatcagct cgcccggctt
     2581 ggcaacgtcc cggcgctgca ttccatgctg cgaagcaggt tcgtgcccgg cgagcgaagg
     2641 acgcggccat gatgacgtca ggagcaacgc catgagcgtg aagatcaacg gtaacggcgc
     2701 cgcagccaac gctttcatcg agaaggttga aagcctgagc aagcaggatc ttctggcctg
     2761 ctatcagtgc ggcaagtgct cggctggatg ccccatggcg aaatacatgg acatccttcc
     2821 ccaccagatc atccgcttcg cacagctcgg cctgaaggac gagctgctcg cctccgaggc
     2881 gatctggatg tgtgtgtcgt gcctcacctg caatacgcgc tgccccaagg gcgtgcgcat
     2941 cgccgaggtt atcgaggccg ttcggcaggt tcgtctgcgc aaaaggcgca acgatcatct
     3001 gattgtgcgg gagctgcccg cgagcgcgct ggcgacgttg ccgccaatcg ccatcatcgg
     3061 cagcatgcgt aagggaactt cgtaagcaag ctcgacagca tcttccccaa aggcaaaacg
     3121 gctcgctttt gcgagcagct tcgccgattt tgagcagcgc agtgacggta gtgggctcta
     3181 atcgatctcc tctgcctgaa gtaatccgcg cttctccatg agcggctcga cttgagggtc
     3241 tcgtccccgg aaagcgcggt aggcctcctc cggtttcata gtgccgccag aagagtagac
     3301 gaaccttcgc aggcgctccg caaccgccgg atcgaaaata tcgcccgctt cctcgaaggc
     3361 ctcgaaagca tcggcttcca tcacctccgc ccacagatag gtgtaatagc ccgctgcata
     3421 gccgctgctt gcgaagatat gctggaaatg aggcggccga tgcctcagcg aaatggcgtc
     3481 cggcatgccg atccgtgcgc gcatatcctg ttcaaactcg cctgcgtcca gagcgcctgg
     3541 cgaggccacc tcatggatat cgaggtcaaa cagcgccgac gccgtatact ccgcggtcgc
     3601 gaacccctga ttgaacttcc gcgcggcgtg aagcttctcg accaaactct gagggatagg
     3661 gtcgcctgtt tcgccatggc gcgcgaagcg gttcaagact tcgggcttca ggagccaatt
     3721 ctcgaaaagc tgagacggca gctcgacaaa atcgcggtcc acgctggtgc ctgcaagact
     3781 gccgtaagtc acatcagaaa gcaggccgtg cagagcatgt ccgaactcgt ggaagagagt
     3841 gcgggcatca tcgaggctca gaagcgtcgg agtgccctgc cctcccttcg tgaagttcaa
     3901 gacattcacg ataatgggaa ccacctctcc gtccagccgc tcctgtctgc gaaaagcgct
     3961 catccaagcg ccgcttcgct tcgaaggcct gtggaagtag tcgcccatga aaagggcaat
     4021 caccttgcct tcgcggttca acacctccca gacacggacg tccgggtgat agacaggcat
     4081 atcgccgcgt tcgttgaaac caagcccgaa aagccgatgc gccgtgtcga acgccgccgc
     4141 gatcatgtta ttcagctcga aatacggctt cagcgccgca ccatcgatat cgaattcggc
     4201 cttccggcgc ttctcggtgt agtgatgcca gtcccaggcc gctagcggct cattatggcc
     4261 tgccgcctgc gccatgcttt gcagcgcttc ccgctcttcg tcggccctaa gcttggctcc
     4321 gtcccaaacc ctttccaaaa gctcgcgcgc ggaggctggc gtcttcgcca tcgcgtcgtc
     4381 gatctggtaa tccgcgtagg tctcgaagcc gaggagcgtc gccagttcac cgcgcaacgc
     4441 cagaatttcc tggatgagcg ggcggttatc agccgccgca tcctcaccgc gcgcga
//
LOCUS       DATMGX010000038         3543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_471036, whole genome shotgun sequence.
ACCESSION   DATMGX010000038 DATMGX010000000
VERSION     DATMGX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471036"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1303
                     /locus_tag="VNR65_00640"
     CDS             194..1303
                     /locus_tag="VNR65_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HWJ17210.1"
                     /translation="MKSRAAVAFEAKKPLEIVEVDLEGPKAGEVLVEIKATGICHTDA
                     YTLDGFDSEGIFPSILGHEGAGIVREVGAGVTSVKPGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRATQGKGLMPDDTSRFSYKGQTIYHYMGCSTFSNFTVLPEIAVAKIREDAP
                     FESSCYIGCGVTTGVGSVVFSAKVEPGANCVVFGLGGIGLNVIQGLKMVGADKIIGVD
                     INSDKEEWGRRFGMTHFVNPKSVQGDIVAHLVELTGGGADYTFDCTGNVNVMRQALEC
                     CHRGWGQSIIIGVAEAGKEISTRPFQLVTGRVWKGSAFGGARGRTDTPKMVDWYMEKK
                     IEIDPMITHKMPLERINEAFDLMHQGKSIRSVVVF"
     gene            complement(1527..2153)
                     /gene="gfa"
                     /locus_tag="VNR65_00645"
     CDS             complement(1527..2153)
                     /gene="gfa"
                     /locus_tag="VNR65_00645"
                     /EC_number="4.4.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051907 - S-(hydroxymethyl)glutathione
                     synthase activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione synthase"
                     /protein_id="HWJ17211.1"
                     /translation="MFWRKKESEPERKTIRPSNIRIHPTVDNGVKPGRSDFAGGTLVC
                     KCKDKPVTVTVGSQTAHNHACGCTKCWKPAGSLFSVVAVAPRDKVKVTANGDKLQIVD
                     PAAAIQRHACTGCGVHMYGRIENTKHPFYGLDFIHTELSPQHGWSEPGFAAFVSSIIE
                     AGADPAQMGAVRGRLKELGLEPYDCLSPPLMDAIATHVAKASGVLKAA"
     gene            2389..2748
                     /locus_tag="VNR65_00650"
     CDS             2389..2748
                     /locus_tag="VNR65_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17212.1"
                     /translation="MKMLRFFTILVAIFILPNVSNAAEGKGIRFWNLTAATITSLQLS
                     PAGKSEWGQNQTDNDKDHSVDHDERLKITGIEPGVYDVKLRDNLHRECVIKNIDIKTD
                     GIFSIEEKKLVGFCHMF"
     gene            complement(2745..>3543)
                     /gene="fghA"
                     /locus_tag="VNR65_00655"
     CDS             complement(2745..>3543)
                     /gene="fghA"
                     /locus_tag="VNR65_00655"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR02821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HWJ17213.1"
                     /translation="VYRHASSETHTPMRFSAYVPPQAQQGSRLPVVWWLSGLTCTEEN
                     FTVKAGAQRVASELGLLVIAPDTSPRGEGIPGDPNNAYDFGLGAGFYVDATVEPWSRN
                     YRMRSYIEQELPALVEAELPADMSRQSILGHSMGGHGALTIALRNPGRFKAVSAFAPI
                     SSPMRCPWGEKALGGYLGKDREAWKTYDACALIEGGARLNDLLVDQGLGDGFLHTQLK
                     PELLQEACDKAGIPLTLRLQGGYDHSYFFISTFIEDHLRWHAVRLGR"
BASE COUNT          774 a         1041 c         1034 g          694 t
ORIGIN      
        1 cacgccgagg cgcgcgacac gaaaggcagg gtgttcaagg cgaagtggcc ggtcgagtct
       61 ggcgcgatgt aatcgctcgg ctgaatacga ggtgggtagc cccttcacgt tgaaagacgg
      121 aagtaccagg agtaaagtcc tttttagatc aataacaaaa ccgcttttgg aagcgttata
      181 agaggaggtt gaattgaaat cacgcgccgc cgttgctttt gaggcgaaaa agccgcttga
      241 aatcgtcgaa gtcgatctgg aagggccaaa ggccggcgag gtgctcgtcg agatcaaagc
      301 caccggcatc tgtcataccg acgcctatac cctcgacgga ttcgacagcg agggcatctt
      361 cccctccatc cttggccatg aaggcgcggg catcgtccgc gaggtcggag caggcgtcac
      421 cagcgtgaaa cccggcgatc acgtcatccc gctctacacg ccggaatgcc gccagtgcaa
      481 atcctgcctc agccgcaaga ccaacctttg caccgcgatc cgcgccacgc agggcaaggg
      541 gctgatgcct gacgacacaa gccgcttctc ctacaagggg cagacgatct accactatat
      601 gggctgctcc accttctcca acttcacggt gctgcccgag attgcggtcg cgaaaattcg
      661 cgaggacgca cccttcgaat cctcctgcta tatcggctgc ggcgtcacca cgggcgtcgg
      721 ctcggttgtc ttctctgcca aggtcgagcc gggcgcgaac tgcgttgtgt tcgggcttgg
      781 cggtatcggc ctcaacgtga ttcaggggct gaagatggtg ggcgccgaca aaatcatcgg
      841 cgttgacatc aactccgaca aagaagagtg gggccgccgc ttcggcatga cccatttcgt
      901 caacccgaaa agcgtccagg gcgatatcgt cgcgcatctc gtggaactca cgggcggcgg
      961 cgcggactac acattcgact gcacaggcaa cgtcaacgtc atgcggcagg ctctcgaatg
     1021 ctgccatcgc ggctggggcc agagcatcat catcggcgtc gcggaagcag gcaaggaaat
     1081 ctcgacgagg ccgttccagc tcgtgacggg ccgcgtctgg aagggttcag ctttcggcgg
     1141 cgctcgcggc cgtaccgaca ccccaaaaat ggtcgactgg tatatggaga agaagatcga
     1201 gatcgatccg atgatcacgc acaagatgcc gctcgagcgg atcaacgagg cgttcgatct
     1261 gatgcatcag ggcaagagca ttcgctcggt tgtggttttc tgaacgtaac tccgctaatc
     1321 cggccagcgg attgccaagc gcagcgcgag ccgggatcgc aaaaaggttg gcgcttcaat
     1381 ttgttacgat cccggagaag acttcgcctt tccgggatca cgacgcgtcc gctaaactct
     1441 gcgtagcact tatagattgg ccgggctcga cccggccaat ctgcatctgc gaatagcccc
     1501 gcatatgcgg ggctatgaac agcgcgtcag gcggccttca ggacgcccga agccttagcg
     1561 acgtgcgtcg cgatggcatc catcagcggc ggagacagac agtcataagg ctcgaggccg
     1621 agttccttca acctgccgcg cacggcgccc atctgcgccg gatcggctcc cgcctcgatg
     1681 atcgaggaga cgaaggccgc gaacccaggc tccgaccaac cgtgctgcgg cgacagctcg
     1741 gtgtggatga agtccagccc atagaacgga tgcttcgtat tctcgatgcg gccgtacata
     1801 tgaacgccgc agccggtaca ggcatggcgc tggatcgcgg ccgcaggatc gactatctgc
     1861 agtttgtcgc cattggccgt cactttgacc ttatcgcgcg gggccacggc aacgactgag
     1921 aaaagagatc ccgcaggctt ccagcacttc gtgcaaccac aggcgtgatt gtgagctgtc
     1981 tgcgagccca ccgtcacggt gacgggcttg tccttgcatt tgcagacgag cgtaccgcct
     2041 gcaaagtccg accggcctgg cttcactccg ttatccaccg tggggtggat gcggatgttg
     2101 ctgggcctga tggttttcct ctccggttcc gattcttttt tacgccaaaa catgtttctt
     2161 ccttggctat tggagttgaa cgttttgctt tttgagcata ctgcaactca cggtgccgaa
     2221 gaatcggcgc cgctcgggct cccgcgaaaa aatcgttccg cccgcgcccc attgtagtct
     2281 tatctcgggc gaattgatat atggtcccgc gatagaaggt tttctacttt ggcttcaggt
     2341 aaccagcgtc ggtacgcgag ttggactatt cgcatcatcg aaggtcatat gaaaatgctc
     2401 agatttttta cgatcctggt agccatattc atcttgccga atgtctctaa tgcggcggaa
     2461 ggaaaaggta tccgcttctg gaatctgacg gcggcgacga tcacaagctt gcagctatcg
     2521 cctgccggaa aaagcgaatg gggccaaaac caaacggata atgacaagga ccacagcgtc
     2581 gatcatgacg agcgcctcaa gatcacgggt atcgagcccg gcgtctatga cgtgaagttg
     2641 cgggacaacc tgcatcgcga atgcgtgatt aagaacatcg acatcaaaac cgacggtatc
     2701 ttctcgatcg aggaaaaaaa gctcgtcggc ttctgccaca tgttctagcg accgagcctt
     2761 actgcgtgcc agcgcaggtg gtcctcgatg aaggtcgaaa taaagaaata ggaatggtcg
     2821 tagcccccct gcagccgcag cgtgagcgga atccctgcct tgtcgcaggc ctcttgcagc
     2881 aactcaggct tcaactgcgt atgcagaaaa ccatcaccca gcccctgatc gacgagaaga
     2941 tcgttaagcc tcgccccgcc ctcgatcaaa gcgcaggcat cgtacgtttt ccaggcctcg
     3001 cgatccttcc ctagatagcc gccaagggcc ttttcgcccc aagggcatcg catcggcgat
     3061 gagatgggag cgaaagcgga tacggctttg aaccggcccg gattccgcaa cgcaatggtg
     3121 agcgcgccat ggccgcccat cgagtgaccc aatatgcttt gcctcgacat atcggccgga
     3181 agctcggcct cgacgagcgc gggcaattcc tgctcgatgt aggatctcat gcgataattg
     3241 cgggaccacg gctcgactgt cgcatccaca taaaaaccgg caccgagacc gaaatcatag
     3301 gcgttgttcg gatcgccggg aataccctcg ccgcgcgggc tcgtatccgg cgcgatgaca
     3361 agaagcccaa gttccgaggc gacgcgctgc gcaccggctt ttacggtgaa gttctcctcg
     3421 gtgcaggtca agccggagag ccaccagacc accggcaggc gactgccttg ctgagcctga
     3481 ggcggcacat aggccgaaaa gcgcatcggc gtgtgcgttt cactggaggc gtggcgatat
     3541 acg
//
LOCUS       DATMGX010000039         2020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_24844, whole genome shotgun sequence.
ACCESSION   DATMGX010000039 DATMGX010000000
VERSION     DATMGX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24844"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VNR65_00660"
     CDS             <1..1511
                     /locus_tag="VNR65_00660"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HWJ17214.1"
                     /translation="GLNPILQDPALAIHPPLLYTGYVGFSMAFSFAIAALLLGRIDSA
                     WARWVRPWTLAAWIFLTVGIAVGSWWAYYTLGWGGWWFWDPVENASLMPWLTGTALLH
                     CAIVMEKRDSLKAWTILLAILTFSFSLLGTFLVRSGVLTSVHSFASDPARGVFILVIV
                     STFTAGGLALFAWRSSALSASTAFSIISRESSLVLNNLLLTVSCGIVLVGTLYPMALE
                     ALTGDKISVGGPFFNSTVVWIFMPLLLALPFGLFLAWKRGNLGEAAKRLSVAAIIAVI
                     AGATAIAFGADAKQGLAITLGAWVLAGSVWEVLWRARYLSVPLSETARRILNMRRGQF
                     GATLGHIGLAVTVIGIAGATAWNVERLAVMKIGDTIDLGTYQLTFKKVYEQAGPNYAE
                     FAGDFDLSSGGSQIGTITTSKRKYDAPPQVTTAAGISSRPAGDVYVVLGNEEKGAFSI
                     RAYYHPFVRWIWGGALIMFLGGLVSLLDRRLRIGLPQRAAKAPFPSAPAPAE"
     gene            1515..2003
                     /locus_tag="VNR65_00665"
     CDS             1515..2003
                     /locus_tag="VNR65_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HWJ17215.1"
                     /translation="MRRTFPSSIVSLLLALFAGSALAVDPQEQLKDPNLEARARDISA
                     GLRCLVCQNQSIDDSDAPLAKDLRLLVRQQLKKGESDQQVVDYVVARYGEFVLLKPRL
                     HLNTILLWFAPIVLVAGGLLLASRVVRRPPPESPARPLTPEEQAELDSLLSPTADKAL
                     SK"
BASE COUNT          334 a          633 c          599 g          454 t
ORIGIN      
        1 aggggctgaa cccgatcctc caagatcccg cgctcgcgat ccatccgccg ctcctctaca
       61 ccgggtatgt cggcttctcg atggcctttt ccttcgccat cgcggcgctc ctgctcggcc
      121 gcatcgactc tgcctgggcg cgctgggtcc ggccctggac gcttgcggcc tggatctttc
      181 tcaccgtcgg catcgcggtc ggctcgtggt gggcctatta cacactgggc tggggcggct
      241 ggtggttctg ggacccggtt gaaaacgcct cgctaatgcc ttggcttaca ggaacggcgc
      301 ttctgcactg cgccatcgtg atggagaagc gggacagcct caaggcttgg accatccttc
      361 tcgcgatcct caccttcagc ttcagcctcc tgggaacgtt cctcgtccgc tccggcgtcc
      421 tgacgtccgt gcactccttc gcgtcggacc ccgcgcgcgg cgtctttatt cttgtcattg
      481 tcagcacctt cacagcgggt gggttggcat tgttcgcctg gcggtccagc gcactttccg
      541 ccagcaccgc attttccatc atctcccgcg agagcagcct cgtcctcaac aacctgcttt
      601 tgactgtgtc atgcgggatc gtgctcgtcg gaacgctcta cccgatggcg ctcgaggcct
      661 tgaccggaga caagatctcc gtcggcgggc cattcttcaa ttctacggta gtctggatat
      721 tcatgccgct gctgctggcg cttcctttcg gtctcttcct cgcctggaag cgaggaaact
      781 tgggcgaagc ggcaaagcgg cttagcgttg ccgccatcat tgccgtgatc gcgggtgcca
      841 cggcaatcgc atttggcgca gatgcaaagc aaggcttggc gattacgctc ggagcctggg
      901 ttctcgcggg ctcagtttgg gaagttttgt ggcgggcgcg ttacctgtcg gtgcccttat
      961 cagagacagc gcgccgaatt ttgaacatgc gccgcggaca gttcggggcg acgctggggc
     1021 atatcggcct tgccgtcacc gtcattggca tcgccggggc caccgcctgg aatgtcgagc
     1081 gtcttgctgt catgaaaatc ggcgacacca tcgatctcgg cacctaccag ctgacgttca
     1141 agaaagtcta tgagcaggcc ggcccgaact atgccgagtt tgcaggcgat ttcgatttgt
     1201 cgagcggggg cagtcagatt ggcacgatca caacgtcgaa gcggaagtac gatgcgccgc
     1261 cgcaggttac aaccgcagcc ggcatttcgt cccggcccgc gggcgatgtc tacgttgttc
     1321 tcggcaacga agaaaagggc gcgttctcga tccgagcgta ttatcatcct tttgtgcggt
     1381 ggatctgggg tggagcgctc attatgttct taggcggcct tgtctcgctg ctcgaccgaa
     1441 ggctgaggat cggcttgcct caaagggccg cgaaagcgcc attcccctca gctcccgcac
     1501 cagcggagta agaaatgcgc cggaccttcc cctcctcgat tgttagcttg cttctcgcgc
     1561 ttttcgcagg atcagccctc gcggtcgatc ctcaggagca gctcaaggac ccaaatcttg
     1621 aagcgcgagc gagggacata tcggcggggc ttcgctgcct tgtctgccag aaccaatcga
     1681 tcgacgattc cgacgctccg ctcgcgaagg acttgcggct tctcgtgcgg cagcagctga
     1741 aaaagggcga gagcgatcag caggtggtcg actacgtcgt cgcgcgctac ggagaatttg
     1801 tgctgctgaa gccgaggctc catctcaata cgattctact ttggtttgcg ccgattgtgc
     1861 tcgtcgccgg cggtcttctc ctcgcctcgc gcgttgtgag acgccctccg ccggaaagcc
     1921 ctgcgcggcc cctgacgcct gaagagcaag cggaattgga ttcgctgctc tctcccacgg
     1981 cggacaaggc cctttcaaaa tagcccgcta aatatttctg
//
LOCUS       DATMGX010000040         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_284647, whole genome shotgun sequence.
ACCESSION   DATMGX010000040 DATMGX010000000
VERSION     DATMGX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284647"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VNR65_00670"
     CDS             <1..716
                     /locus_tag="VNR65_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ17216.1"
                     /translation="GYFRMVVANTGTRQVGVVVEEVLGKDEIVIKSLGEYLRRIKLFP
                     GTTIAPDGSLILLLDLNRMVASEPNERRAVQASASAARVFAPGSAAIAQGSIPTDAID
                     RVDQERVVVIVDDSISVRKFVGRMLEKNGYRVKLAADGLEAAELITQQGCHLVITDLE
                     MPRMTGYELMAQLRQNPITRRIPVMVVTSRAGAKHRERALKEGATSFLTKPVQEDQLL
                     FAVEQLIGTEAARPVVPVG"
     gene            724..1857
                     /locus_tag="VNR65_00675"
     CDS             724..1857
                     /locus_tag="VNR65_00675"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HWJ17217.1"
                     /translation="MVSNAPKKFRVLVVDDSAFMRKVLESIFNSDEQLQVIGHAKDGR
                     EAIALSESLKPDVITMDINMPHVDGLQATAHIMTSNPRPIVVVSSETREGAFSTLKAL
                     ELGAIEFVTKPSSGIDLDMQSIREDLVRKVRMAAKVRVVRTASRQPSANHAPVVKAPL
                     VSSDSPPLAQRAAAANSDFRFPVVVLAASTGGPATIMRMAPGFTRDFPAAVFLVQHMP
                     ATFTTQYAVQLAEFTNIRVKEAEHGEAVQPGTLYICPGGQHLRVTQTGRIQLDATTGR
                     IEGYLPNIDVTLESIAAFAGPMGIAAILTGMGSDGARGARLIKSAGGTVLAQDEATSV
                     IFGMPAEAIKTGAVEQVLGIDDIYPAIEKRVLSLGRAMPAEVR"
     gene            1857..>2138
                     /locus_tag="VNR65_00680"
     CDS             1857..>2138
                     /locus_tag="VNR65_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17218.1"
                     /translation="MRFESTHKSPTVRSEQIILFRCSSQLLAISSASVQEVRSVDSLA
                     GVASEIIVPGVRTVRHALQRGDRSIYVVNVALHFGLKPSPASLIFLLRKS"
BASE COUNT          465 a          615 c          609 g          449 t
ORIGIN      
        1 acggctactt tcgcatggta gtggccaata ccggcacgcg gcaggttggc gtagtcgtcg
       61 aagaagttct tggcaaggac gagatcgtta tcaagagtct tggagaatat ctacgccgca
      121 tcaaactgtt tcccggcacg acgattgcgc cagacggtag cttgattctc ttgctcgacc
      181 ttaaccgcat ggtcgcatcc gagccgaatg aacgccgcgc cgtgcaggcc agcgcaagcg
      241 cggcgcgggt atttgccccg ggttctgccg ctattgccca aggcagtatc cctactgacg
      301 ccatcgatcg cgtcgaccag gagcgggtgg tcgtcatcgt tgatgattcc atcagcgtgc
      361 gcaaattcgt gggacgcatg ctggagaaga atggttatcg tgtcaagctt gccgcggatg
      421 gattggaagc cgccgaactc atcactcagc agggttgcca tttggtgatt accgacctgg
      481 aaatgccccg catgacggga tatgagttga tggcacagct gcggcaaaac cccataacgc
      541 ggcgaatccc cgtcatggtt gtcacgtcgc gcgcaggcgc caaacatcgt gagcgagcgt
      601 tgaaagaagg cgccacttcc ttcctgacga agccggtgca ggaagatcag ctcctgttcg
      661 ccgtggagca actcatcgga acggaggcgg cacgccctgt cgtgccggtg ggatagacga
      721 gccatggtca gcaacgcacc caaaaagttt cgcgtgctcg tcgtggacga ttccgctttc
      781 atgcggaaag tcctcgaatc cattttcaat tctgacgaac agttacaagt aatcggccac
      841 gcgaaagatg gccgcgaagc catagcgctg tcggagtctc tgaaaccgga tgtcatcacc
      901 atggacatca atatgccgca tgtggacggc cttcaagcca ccgctcatat catgacgagc
      961 aacccacgtc cgatcgtggt cgtaagctca gaaacacgcg agggcgcatt cagcacgttg
     1021 aaggctctgg aacttggcgc gatcgaattt gtaaccaagc catccagcgg aatcgacctc
     1081 gatatgcaga gcattcgcga agacctggtc cgcaaggtgc gcatggccgc caaagttcgc
     1141 gttgttcgca cagcttcacg ccaaccaagt gccaaccacg ccccggttgt gaaagcacct
     1201 ctggtcagtt ctgattcgcc ccccctggca caacgtgctg ctgcggcgaa ctcggacttc
     1261 cgtttccctg ttgtagtgct ggcggcttcc acgggcggcc cggcgacgat catgagaatg
     1321 gcgccgggat tcacgcgtga ttttccggct gcagtttttc tagtgcagca catgccggca
     1381 acgttcacta cgcaatacgc agtgcaactc gcggagttca caaacattcg tgtaaaagaa
     1441 gcggaacatg gcgaagcggt gcaacctggc actctataca tctgtccagg aggccagcac
     1501 ttgcgcgtga cgcaaactgg tcgaattcaa ctcgacgcca ccactggacg catcgaaggg
     1561 tatctcccta acatcgatgt gacactagaa tcgattgcgg ctttcgcagg cccgatggga
     1621 atcgccgcga tcctgaccgg catgggcagc gatggtgcgc gaggagcacg gcttatcaaa
     1681 tccgccgggg gtactgtcct ggcccaagat gaagcgactt ccgttatttt tggcatgccc
     1741 gcggaagcga tcaagaccgg cgccgtggaa caagttttgg ggattgatga tatttatccc
     1801 gcgattgaaa agcgcgttct aagcctcggc cgggccatgc ccgcggaagt gcgttgatgc
     1861 gattcgaatc cacacacaaa tcacccaccg tgcgcagcga gcagatcatt ttgtttcgct
     1921 gtagcagcca actcctggcg atttcgtcag cttccgtgca ggaagtccgt agtgtggaca
     1981 gcctcgccgg tgtcgccagt gaaatcatcg tgcccggtgt ccgcaccgtg cgccatgcac
     2041 tgcagcgggg tgatcgttcg atctatgttg tcaatgtcgc gctgcacttt ggattgaagc
     2101 cttcacccgc atcgctgatc tttctacttc gcaaatcg
//
LOCUS       DATMGX010000041         4824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_404490, whole genome shotgun sequence.
ACCESSION   DATMGX010000041 DATMGX010000000
VERSION     DATMGX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404490"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VNR65_00685"
     CDS             <1..571
                     /locus_tag="VNR65_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HWJ17219.1"
                     /translation="IGPLIQFDPIMGANGPLFVHRLEFQILAPTGDYDRYAAVQPGAN
                     FWSFDPYWAGTVFITPEWTASARIHYLWNGKNDNPNASLQRELPGVTIASSQAGQAFH
                     MNFATEYAVTKELRIGLNGYYLQQTTDTEVNDGITLPNRKERVAAIGPGFVYSWGPEH
                     TFFFNAYQEFGAENRPEGERFTVRYVQHF"
     gene            767..1177
                     /locus_tag="VNR65_00690"
     CDS             767..1177
                     /locus_tag="VNR65_00690"
                     /inference="COORDINATES: protein motif:HMM:NF022467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HWJ17220.1"
                     /translation="MHLAERLDDIGKPAWIALMIVSFIIWWPIGLATLAFLFWSGRMG
                     CGHHGHMSWQERRERWREARDEWRAMKRGAYGAMRSSGNAAFDDYRSETLKRLEEEQK
                     EFTGFLDRLRRAKDQAEFDQFMTERRNKPAGEPS"
     gene            complement(1436..2173)
                     /locus_tag="VNR65_00695"
     CDS             complement(1436..2173)
                     /locus_tag="VNR65_00695"
                     /inference="COORDINATES: protein motif:HMM:NF017672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HWJ17221.1"
                     /translation="MIMSSWFGDMTRQIDMYCERRGTAWDAEPLNAVSNAAFFIAAWA
                     AWRLCERHPNAAFGGAIRALCIIIAVVGAGSLTFHTLATRWAEWADVIPILIFMIVYC
                     WLILTAFFRWPWWAKSLATLLLFAATFYLESDEFEKVLWGGAMYLPTLVFLMAVGAGI
                     WRGNARAGKAFYAAAGLFVLSFTARTLDRPMCDALPIGTHYFWHLFNAAVLFLLVRTV
                     IVHAPPPEEKAFGSWGTEPTVLGAPGT"
     gene            complement(2340..3092)
                     /locus_tag="VNR65_00700"
     CDS             complement(2340..3092)
                     /locus_tag="VNR65_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HWJ17222.1"
                     /translation="MKVGFRLLCALAASVTLGSYSAPSRAAPLCDGPPPAIWPQLPEL
                     PKGCQRERIQASGELSFNVLRKASKIAEAAWQREVITKYGERFQDLEFAACKNVLCVK
                     GAVSGTERCTISAFPCASDMGVPARAQVERLAGLDDRNLSPEDIRRAEHAEERREAIQ
                     EVKREERREERREDRVEERRDDRIVGRDLNPQEVREMQHLLNRAGYRVQSDGVFGDES
                     RDALSAWERRAGMRDDGYPTYEALERLRRDIR"
     gene            complement(4047..>4824)
                     /locus_tag="VNR65_00705"
     CDS             complement(4047..>4824)
                     /locus_tag="VNR65_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWJ17223.1"
                     /translation="VTGPEGSDPICHVLPAWDCITGQMAALSLLAAERHRRLTGEGQL
                     VELALKDVALAMLGNLGIIGEVTINQYDRPKFGNALYGAYAQDFLCADGKRVMVAAIT
                     PRQWDGLVAVTGMAAEFQQLSQKLGLNLGLEGDRFKARAGITELLKPWFAARRVDDFA
                     RSLNESGITWSVFRSFAQTIAEDPDCSTANPMFSVLEQPGIGSYLVPGSPLSFSAYDR
                     EPPTRAPLLGEHTDAILHEIGYSDSEIGALHDAKVVAGPS"
BASE COUNT         1051 a         1395 c         1375 g         1003 t
ORIGIN      
        1 gatcggcccg ctcattcaat tcgaccctat catgggcgcg aatgggccgc ttttcgtcca
       61 ccggctcgaa tttcaaattc tggcgcctac gggtgattat gacaggtacg cagccgtaca
      121 gccgggcgcg aacttctggt ccttcgatcc ttactgggcc ggcactgtgt tcataacgcc
      181 cgaatggacg gcgtcggccc gcattcacta tctgtggaac ggcaagaacg acaacccgaa
      241 tgcgtcgctc caaagggagc tccccggcgt caccatcgcc tcgagccagg cgggccaggc
      301 cttccacatg aacttcgcaa ctgagtacgc ggtgaccaag gaactgcgga tcggcctgaa
      361 cgggtattac ctgcaacaga cgaccgatac cgaggtcaac gatggcatta ctttgccgaa
      421 tcgcaaagaa agagtggcgg ccatcggtcc agggttcgtg tacagctggg gcccggagca
      481 tactttcttc ttcaacgcct accaggagtt tggcgccgag aaccgtcccg aaggcgagcg
      541 cttcaccgtg cggtacgtcc agcattttta atggttgggc gccgcggata ctaaaatagc
      601 aggaattctt gaggggtggg ttggctgtcg cgcgctgtgc ggcagccaat tttcttagct
      661 gccctgtttt aggcaaatga gaaagtcgcg ccggttctct tgacaaagcc accacggtcg
      721 cgcacctatg ttaatgtaac ttacatttac atagggagtt acgcaaatgc acctagccga
      781 aaggctcgac gacatcggca aacccgcatg gatcgcgctg atgatcgttt ccttcattat
      841 ctggtggccg attggcttgg ctaccttggc tttcttattc tggagtggac gcatggggtg
      901 tggacatcac gggcacatga gttggcagga gcgccgcgag cgctggcgtg aggcgcgcga
      961 tgagtggcga gccatgaagc gtggcgctta tggcgcgatg cggtcgagcg gcaatgccgc
     1021 cttcgacgac tatcgctctg agacgctgaa acggctcgaa gaggagcaaa aagagttcac
     1081 cggctttctc gatcgattgc gccgcgcgaa ggatcaggcc gagttcgatc agttcatgac
     1141 cgaacgccgc aacaagccag ccggtgagcc ttcataggaa aagagcgtcg ctttgaaaat
     1201 gagcatcgct ttccggtcat agaactcaga agaagtccaa ggacagagat ggcgaagcag
     1261 tattgccgtc tctgttaagc gaccatcaga tccgcgccat cgatcccgtc ctggatcgcc
     1321 gcaggcctca tgccggtgga accttatccg ggatcgtaac catggaagtt tgcgatctcc
     1381 cggctcgtgc tttgcttggc cgggatgaca acccgttgac cagattggcg tttgctcagg
     1441 tgcccggcgc accaaggacc gtcggctccg tgccccacga cccgaaggcc ttttcctcgg
     1501 ggggaggggc atgaacgatg acagtccgga ccagcaggaa aagcaccgcg gcgttgaaga
     1561 gatgccagaa atagtgggtg ccgatgggaa gggcgtcgca catcggccta tcgagagttc
     1621 ttgcggtgaa ggaaagcacg aaaagtccgg ccgcagcgta gaaggctttg cctgcgcggg
     1681 cgttgccgcg ccagattccg gcgccgacgg ccatcaggaa gaccagcgtc ggcaagtaca
     1741 ttgcgccgcc ccagagaact ttctcgaact catccgactc caggtagaac gtggccgcaa
     1801 aaagaagaag cgtcgcgagc gacttggccc accatggcca ccggaagaac gccgtcaaaa
     1861 tcagccagca atagacgatc atgaaaatca ggatcggaat aacatccgcc cattcggccc
     1921 agcgggttgc gagggtgtgg aaggtcaggc tgccggctcc gaccacagcg atgatgatgc
     1981 agagcgcccg gattgcaccg ccgaacgcgg cattcgggtg gcgctcgcac aaacgccaag
     2041 ccgcccaggc cgcgatgaag aatgcggcat tggaaacggc gttgagcggt tccgcgtccc
     2101 aagcggttcc gcgtcgctcg caatacatat cgatttggcg ggtcatgtcg ccgaaccagg
     2161 aactcatgat catgacgcct atcccccgca gccccgtttt tgccttatat ccaattggag
     2221 ctttagccca ggcgctgacc ccgagcaata cgaacgggat ctgaggcctt cacggttttc
     2281 ctaaaacaca aaagcggagc gcattgcgct ccgcaaaatc aactcccaaa cggcgatcgt
     2341 tacctgatgt cgcgacggag cctttccaga gcttcgtagg tgggatagcc atcgtcacgc
     2401 atcccggccc tgcgctccca ggcgctgaga gcatctctcg attcgtcgcc aaaaacgcca
     2461 tcagactgga cacgataacc tgcccggttc agcaggtgtt gcatctcacg aacctcttgc
     2521 gggttgagat ccctccccac aatacgatcg tcccttcttt cttccactct atcttctctt
     2581 ctctcttccc gtctctcttc cctttttacc tcctggatgg cctctcttcg ttcctcagcg
     2641 tgctcagcac gccgaatgtc ctcgggagac aaattccggt cgtcgaggcc tgccagacgc
     2701 tcaacctgcg ccctcgccgg tactcccatg tccgatgcac aggggaaggc agagatcgtg
     2761 cagcgctccg tgccgctaac cgcgcccttc acgcagagga cgttcttgca ggccgcgaat
     2821 tccaaatcct ggaagcgctc gccgtacttc gtgatcactt cacgctgcca ggcagcctcg
     2881 gcaatcttgc ttgccttgcg aagcacattg aagctgagtt caccggaagc ctgaatccgc
     2941 tccctctggc agcctttcgg cagttcaggt aactgcggcc atatagccgg cggtggacca
     3001 tcgcagagag gcgccgctct tgatggcgcg gaatagctcc ccaaagtcac gctcgcggcg
     3061 agagcacata acaatcgaaa ccctactttc ataatcaccc ctccgtccga ttcgaaacaa
     3121 aaatgcggcc tacaaagcat caaccgaaac gcatcggcgc atgatgctgc aatttgtgat
     3181 cctctttggg cacaggctgg gcggttgaac cgcacctaga tcttttattg ggaggcattc
     3241 aagcggacga aaattgcaca gtcaagaatc cattaacgat tgttaacgac ttggggattc
     3301 gatgccacta ctgtgtccga acccgctgaa ttttatcgat ttgaggccat atccgtttgg
     3361 atgaggcgtg cgcgcttcgc tgtattgatc tgcaataaat cgtgaattgc gctactgggg
     3421 gagaatcacg agtataaaca cagtatcccc taagatgctt tccccataag ccagaagctg
     3481 gacctcgctc tatcacatgg agcgaggttt tttctttttg ccaacaataa agtgtttaaa
     3541 aggcttaagt cagcgcattg cgacgggagg caagagtgat tcttccgcga ggttcttttt
     3601 ttgaagtttc atgcaatggc atcgcggctt cggcgagcac ctcgttctgt tgataccagg
     3661 cgtcggcgca agccacgcag caaaacctac gccctgcaag tgtaatatac tcaaactcga
     3721 tgacttcgcc ggagcaaaaa acgttgtcgc ataggaccat gttgtctctc ccgttgtggg
     3781 gcgcgtacgc taacaagaga ctggcactgg gctcgcgctc attaactcga atcaggcgag
     3841 taatattata gcggatatca cggcgctttt cgaaatagag cgcccagctt gggcgctgct
     3901 gtggatgcgc acgagcgtta ttggctgtat tcgaagcgtt ttctgttaga tcaaaccgcc
     3961 tttaatcgaa accgattaaa ggcggaaaag ttgatcgatt ctcaagtgct agagcagctt
     4021 atccgcgtcc ccatgggcgc gggctgctag ctaggcccgg cgacgacctt ggcgtcgtgc
     4081 agtgccccga tttcggagtc ggagtatccg atctcgtgca gaatcgcgtc tgtgtgctct
     4141 cctagcaacg gcgcgcgcgt tggtggctcg cggtcgtagg cgctgaacga aagcggactt
     4201 ccgggaacga gatagctgcc gatcccgggc tgctccagca cgctgaacat cgggttggct
     4261 gtggagcaat caggatcttc cgcgatcgtc tgtgcaaacg atcggaatac gctccaggtg
     4321 atgccactct cattgagtga gcgtgcaaag tcatccacgc ggcgagcagc gaaccacggc
     4381 ttcagaagct cggtaatgcc ggcgcgagct ttgaagcgat cgccctcaag gccgagattt
     4441 aacccaagtt tctggctgag ctgttggaac tcggccgcca tgccggtcac ggcgacgagc
     4501 ccatcccatt ggcgaggcgt gatggccgcg accatgacgc gcttaccatc ggcgcagagg
     4561 aaatcctgag cgtaagcgcc gtaaagggcg tttccaaatt tgggccggtc gtactgattg
     4621 atggtgacct caccaatgat gccgagattg cccagcatcg cgagtgccac atccttcagg
     4681 gcaagctcca cgagttggcc ttcgccagtc aggcggcggt gccgctcggc cgcgaggagc
     4741 gaaagcgccg ccatctgccc ggtgatgcag tcccaggccg ggaggacgtg gcagatggga
     4801 tcggaccctt caggcccggt gacc
//
LOCUS       DATMGX010000042         2837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_470672, whole genome shotgun sequence.
ACCESSION   DATMGX010000042 DATMGX010000000
VERSION     DATMGX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470672"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..2164)
                     /locus_tag="VNR65_00710"
     CDS             complement(140..2164)
                     /locus_tag="VNR65_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ17224.1"
                     /translation="MLSYGTWTRRFGSDPGVLGKKIMLNGVPYEIVGVMPRSFSLARE
                     VMPTLDGAEQAEILLPLPLPGKAAQDRDHEDYNILGKLKPGVSLQQAQAEMDTITASL
                     RHDFPEVYPPNGGLTFGIVPLLDQVVGNVRRTLYLLLGAVGFVLLIACVNVANLQLSR
                     AVARQKEIAVRTALGATRMRIIRQLLTESVLLAFGGGALGIFLAFLALHGVRLLGPQS
                     VPRVNDIGIGLATLAFTVMVSILSAVLFGLAPALRVSRLDVQTALRDNSRTSAGVSAI
                     WGRGNKLRRSLVIAEIALCAMLLIGAGLLIRSFVRVRDVNPGFNPRNVLSLELTMTGE
                     RYKDKQAVLAAYHELWQRLEHLPGVTSAGAVTSLPLSQMFAWGPITVEGRVPPPGEKF
                     INADERMVSGRYFQAMEIPLREGRLFNDDDVADKPRVAIVDDYMAQQLWPSQDPIGKR
                     LHIGGISETSSPWITVVGVVGRVKQYTLDSDSRIAFYLPQTQYVTRAMNVVMRGSSDP
                     VALVGAVKQQIHELNTDLPLYNVGTMQERVDKSLAPRRFIMLVLGTFGAISLGLAMVG
                     IYGLIAYLVGQGSREVGIRLALGATPANIMKLIVSGGMTQAFWGVGIGIGGALIVSRL
                     MRSLLFGVGVADVATFVTVPILLACIALVAGYVPARRASRIDPTSSLRCE"
     gene            complement(2076..2612)
                     /locus_tag="VNR65_00715"
     CDS             complement(2076..2612)
                     /locus_tag="VNR65_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ17225.1"
                     /translation="MNILIQDVRHALRVLAAAPAFTLAAVLSLAIGIGANTAIFSIVD
                     GLLLRPLPYKDAERLVILWNRSPGLDITEDWFSTAQYFDIKSGHHGFEQVAIAIGGNY
                     NLTGWGEPERVGAIRVSSNLLPMLGGRAALGRLFVADEDQPDRTATAPAQLWNMDTAL
                     WLRSRRSWQEDHAERRAV"
BASE COUNT          653 a          858 c          809 g          517 t
ORIGIN      
        1 tcggctccaa cccgatcatg gttcaggtgc ggtagacggt aaatcgatct gcctcagttt
       61 gtagtttggg gcgcctaaga ggatagcgct ggatggacga tcgatgccct gtaaaggcga
      121 ccccttcagc aacgcgtccc tattcgcagc gcaatgacga ggttggatca atgcgggaag
      181 cgcgtcgcgc tgggacatag ccggcgacca aagcgataca agctagaaga atgggaaccg
      241 tgacaaacgt cgcgacatcg gcgactccga ccccgaaaag cagcgagcgc atcaagcggc
      301 tgactatcag cgcgcctcct atgccgatac caactcccca gaaggcctgg gtcatccctc
      361 cgctaacgat aagtttcatg atgttcgccg gtgtggcccc aagcgccagc cggatgccca
      421 cttcgcggct gccttggcca accagatagg cgatgagccc atagattcca accatcgcga
      481 gcccaagtga aatggcccca aatgttccca gaaccagcat gatgaaacga cggggggcca
      541 gagacttgtc cacgcgctcc tgcatggtgc ccacgttgta gagcggaaga tcagtattaa
      601 gttcgtgaat ctgctgcttt acagcgccga ccagcgcaac cggatcgctg ctaccgcgca
      661 taacgacatt catggcgcgc gtcacgtatt gggtttgcgg aaggtagaac gctatgcgtg
      721 agtccgagtc gagggtatat tgcttcaccc ggccaaccac gccgacaacg gtgatccagg
      781 gagagcttgt ctcgctgatt ccaccgatgt gcaagcgctt gccgatcggg tcctggcttg
      841 gccagagttg ttgcgccatg tagtcgtcga caatcgccac acgcggcttg tcggcgacgt
      901 catcatcgtt gaataaccga ccctcgcgaa ggggtatttc cattgcctgg aaataacgcc
      961 cgctgaccat gcgttcgtcg gcattgatga acttctctcc cggcggcggc acgcgtccct
     1021 caacggtgat aggtccccag gcgaacattt gactcagcgg cagcgaggtg acggcccctg
     1081 ccgaggtgac acccggcaag tgttccagcc gctgccatag ctcatgatag gcggcgagaa
     1141 ccgcctgctt gtctttgtat cgctcgcccg tcatggtcag ctcaaggctg agaacattac
     1201 gaggattgaa gccgggattt acgtcgcgta cacgaacaaa gctgcgaatc agcagcccgg
     1261 cgccaatcaa cagcatcgcg caaagcgcaa tctcggcgat caccagcgag cggcgcagct
     1321 tgtttcctct tccccaaatc gcactgacgc ctgccgaagt gcgactgttg tcccgcaggg
     1381 ccgtttgcac gtcgagccgc gaaaccctta gcgccggcgc cagtccaaac aggaccgccg
     1441 acagaatgga aaccatgacc gtaaaagcga gagtggcaag gccgattccg atatcattca
     1501 ctctcggcac gctctgcgga ccgagcagcc gtaccccgtg cagcgcgaga aatgccagaa
     1561 agatgcccaa tgcgcctcct ccaaatgcaa gcaagacgct ctcggtcagc aactgccgaa
     1621 tgatacgcat ccgcgtggcg cctagcgcag tgcggacggc gatctccttc tggcgtgcga
     1681 cagcgcgcga cagttgcagg ttcgctacgt tcacgcacgc gatcagcagg acaaagccaa
     1741 ccgctccgag cagcagatac agcgtgcgcc gaacgttgcc taccacctga tcgagcagcg
     1801 gaacgatacc gaacgtcagg ccgccgttgg gcggatacac ttcgggaaaa tcatgccgca
     1861 ggctcgccgt aatggtatcc atttcggctt gtgcctgttg cagggaaacc cccggcttca
     1921 acttgcccaa tatgttgtag tcttcgtggt cacgatcctg ggcggccttg ccagggagag
     1981 gaagcggcag caagatctca gcctgctccg cgccgtccag cgtcggcatc acctcgcgag
     2041 ccaacgaaaa cgagcgtggc attacaccga caatttcata cggcacgccg ttcagcatga
     2101 tcttcttgcc aagaacgccg ggatcggagc caaagcgccg tgtccatgtt ccataactga
     2161 gcaggggcgg tggcggttcg gtctggttgg tcctcatcgg caacaaacaa tcgcccgagc
     2221 gccgcccgcc ctcccagcat gggcagtaaa ttcgacgaga ctcggatcgc cccgacgcgt
     2281 tccggttcgc cccagccagt gaggttgtag ttgccaccga ttgctatcgc gacctgctca
     2341 aagccgtgat gaccgctctt gatgtcaaag tactgcgccg tggaaaacca gtcttccgta
     2401 atatccaggc caggagagcg gttccagagt atgactagcc tctcagcgtc tttgtaagga
     2461 agtggacgaa gtaacagccc atcgacgatg ctgaaaattg cggtgttcgc gccgattccg
     2521 atggccaacg agagtaccgc cgccaaggta aaggcgggtg cagcggccag aacacgaaga
     2581 gcgtgccgca catcttgtat caatatgttc atgcagaaag tagcggcctc gacggctaat
     2641 gtactccaac agacggacaa agcgcacgat ggaaagcagg ctgctgaaca cgtcgcaacc
     2701 ggacgtaaac aggtggtgag taccgtttgg cgcaacaacc aggctcaagt ccaaaaacgt
     2761 ggtctgccaa cacaaagtta accagagacc ggcaggtggg agatcctccc gataagtcgg
     2821 ccagcatccg gcaaccg
//
LOCUS       DATMGX010000043         2052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_22015, whole genome shotgun sequence.
ACCESSION   DATMGX010000043 DATMGX010000000
VERSION     DATMGX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22015"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..1132)
                     /locus_tag="VNR65_00720"
     CDS             complement(362..1132)
                     /locus_tag="VNR65_00720"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HWJ17226.1"
                     /translation="MKRIKAALAVATLAAITLFWAGHPVRAEERPCKPYSFEGNAYTV
                     CEAPLNRFSIRLFWQKPDGAPYSYLSALPKTDATGGRLAFALNGGMFHPDYKPVGLYI
                     EDGREMVRANTRSGPGNFHLKPNGIFYAGEGEAGVLETSAFLKKKPNALFATQSGPML
                     VVDGKLHPRIAKANVSAKARDGVCVRGAGVVLFAISEGEVPFDTFERLFRDGLKCRNA
                     LFLDGGTAPALYVPSASRSGNMLLGLGPMIAVYDRTRP"
     gene            1413..>2052
                     /locus_tag="VNR65_00725"
     CDS             1413..>2052
                     /locus_tag="VNR65_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWJ17227.1"
                     /translation="MPLRVFICVWKSISADNISLLAAGVAFYMLLAIFPALVFLVAMF
                     GLLTDPDQVRQWLASLQGVLPTEAWNAIDAQVLVLMKQNSASLSLASIFSLLLAFINA
                     RLGAYSMMGALNVVYNTPETRSFTLTNLIAFMFTIAALCVLVFNIYAVIAVPRVLDGI
                     GLTELSNSIVHTMRWPVLAIIMALSLALVYRYGPDREDAHWRWVSIGSLAGTI"
BASE COUNT          412 a          615 c          540 g          485 t
ORIGIN      
        1 gttttgatct gcgaagcatg tccgcgatcg gggcggcgca gcgtgccgct tcatcgcgcg
       61 cgatgcgtgg aggctcggtt aacagacctt gcatattcac ttaaaaaagc gttacggccc
      121 tacgcgcatg gaggttttaa ggcctttgct gagcaacgcc tattttcctg gaattttaaa
      181 taaataacgc cacgcgttat aaagttacgc cttcgtccat ggaattcatc gaaaaattac
      241 ttatataatt acttatcgtt gtctttacga aacgaaccca attcagttaa tcgaccatgt
      301 aatttcacag cacacgatct agaatgcacc ccctccgaat gcggtcgggc gagcaacacg
      361 gtcagggccg cgtcctatcg tagaccgcga tcatcggacc cagcccgagc agcatgtttc
      421 cgctgcgaga ggcagacggc acatagagag ccggggctgt ccctccatca agaaacagcg
      481 cgttccggca tttgaggcca tcccggaaca gacgctcgaa cgtgtcgaac ggcacttcgc
      541 cctccgaaat cgcaaacagc acaacgcccg ccccccggac gcaaacccca tcgcgcgcct
      601 ttgccgatac attcgccttc gcgatcctcg ggtgcagctt gccgtcgacc acgagcatgg
      661 ggcccgattg cgtcgcaaac agcgcattcg gtttcttctt caggaaggcg cttgtttcaa
      721 gaaccccagc ctcaccctcg cctgcgtaga aaatgccgtt cggcttcagg tggaagttgc
      781 ccgggccgga cctggtattc gcgcgcacca tttcgcgccc gtcctcgata taaagcccga
      841 ccggcttgta gtccgggtga aacatgccgc cgttcagggc aaaggcgagc cggccgccgg
      901 ttgcgtccgt tttcggcagc gcactgagat aggaataagg ggcaccgtcc ggcttctgcc
      961 agaacagcct tatcgagaag cggttcaagg gcgcctcaca cactgtgtag gcgttgcctt
     1021 cgaagctata cggcttgcag gggcgttcct cggcgcgcac gggatggccg gcccaaaaga
     1081 gggttattgc tgcaagggtg gccacggcca aagccgcttt tatgcgtttc actttcgctt
     1141 gtccttcgca ggagaacgcc gacttttttg agctggtgtt cagtggctta ccctcgacta
     1201 ttgaagaccc cggctgccgt caaggccaag caacatttgg taggacggga gcgctttacc
     1261 ccgtataagt gtcataccct caattgctgg acctcgcatt cgccaaaggc cagttttcca
     1321 acgatgtccg ggctatggct ccgttactca atctgaaaac cacttcgaaa cggagcctgg
     1381 cggcatcttt tgcatggaag cggcgttata tcttgcctct tcgggttttc atttgcgtat
     1441 ggaaatcaat ttcagccgat aatatcagcc tgcttgccgc cggcgttgcc ttttacatgc
     1501 tgctggcgat cttccccgcc cttgtgttcc ttgttgcaat gttcggtctt ctcaccgatc
     1561 ccgatcaggt gcggcagtgg ctcgcgtctc tgcagggcgt gcttcccacc gaagcctgga
     1621 acgcgatcga cgcccaagtc ctcgttttga tgaaacaaaa tagcgcctcg ctgtctctcg
     1681 cctcgatttt cagcctcctg ctcgccttca tcaacgcccg ccttggggcc tactcgatga
     1741 tgggagcctt gaacgtcgtc tacaacacgc cagagacgcg cagcttcacg ctcacgaacc
     1801 ttatcgcgtt catgtttaca atcgcggcgc tttgtgtctt ggtcttcaat atctacgctg
     1861 ttatagccgt gccacgcgtt ctcgacggca ttggtcttac ggaactttcc aacagcatcg
     1921 tgcacacaat gcggtggccg gtcctcgcca taatcatggc tttgagcctt gcactggtgt
     1981 accggtatgg gccagatcgc gaggatgcgc attggcgatg ggtttcgatc ggctcgctcg
     2041 ccggaaccat tt
//
LOCUS       DATMGX010000044         3189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_470372, whole genome shotgun sequence.
ACCESSION   DATMGX010000044 DATMGX010000000
VERSION     DATMGX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470372"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1254
                     /locus_tag="VNR65_00730"
     CDS             154..1254
                     /locus_tag="VNR65_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ17228.1"
                     /translation="MTQTSLVNQDWPDTVERLGGVAALKESARKTKAFLRGREFDDAI
                     SMLRVILAYCLGDGGLRSTAAWAASIGLADVANTALLYRLRQSGDWLAWLVGQALASA
                     APKASQGRPIRIVDATNVLKAGVAAKTKNKLWRIHSAFDLPSERFGFFELTDEHGGER
                     LDRAPVIKGEIRLGDRVYMQPDRIAALLEAGADIVVRSGWRNARWLDANGAQVDLLAE
                     FEKAAASGRIDSPIWVGRKSAPALALRLVAIKKPPQAAEASCRAAQRSAKKAGNAISE
                     GTLTAAHWIILVTSLPADDFSTDDILALYRLRWRIELAFKRLKSLIGLKAPPGVDERS
                     AKPYVLAHLLVILLLEPLIDEFEDSPRLAVAA"
     gene            1691..2971
                     /gene="nifV"
                     /locus_tag="VNR65_00735"
     CDS             1691..2971
                     /gene="nifV"
                     /locus_tag="VNR65_00735"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR02660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HWJ17229.1"
                     /translation="MRDAFPELRQDEGGAAGGFRPSAEGGLYPHRRLKRRVAINDTTL
                     RDGEQTPGVAFTANEKLQIAASLSDAGVDEIEAGTPAMGAEEQDAIRRIARAGLPIRV
                     MAWCRMRRSDVDDAIASGVGIVNLSAPVSHPQIVAKFGGSVLRVVAATRDVIGYARAK
                     GLEVAFGGEDSSRASRETLEPILEAAAKAGATRYRFADTLGVIDPVATRAAIETVRTM
                     TDLPIEFHAHNDLGFATANTIAAIEAGASAASVTVNGLGERAGNAALEQVAVALDSIF
                     GNGHRVHLPALRHLSALVARSSGIEVGRAQPVVGKDVFTHESGIHVDGILKSRACYEA
                     LSPQKLGRNHRFVLGKHSGLSGLRHELAYLGLQLTEDEERQLLSAVRSYSERQKTTIP
                     AFTLLRLATSLIDERSDDGFRHCGHQAETERNAA"
     gene            2968..>3189
                     /locus_tag="VNR65_00740"
     CDS             2968..>3189
                     /locus_tag="VNR65_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HWJ17230.1"
                     /translation="MSKVISDLEKLHAAEDFFSYLKLDYDPEVLRVCRLHILKRMGKY
                     LGGMDFTGASEDLIFTSAQETLAHAYQDFV"
BASE COUNT          639 a          988 c          944 g          618 t
ORIGIN      
        1 gaaccccata ttgtcatggc cgccctccga ggcggccatc caggataaca aatttcatct
       61 ctttggatct taagactgga tggccgggtc aagccccata ggcgctaaaa taaaaaactt
      121 gccagctaga atcgagtcag attctagcaa ggcatgactc aaacatcgct tgttaatcaa
      181 gactggccag ataccgtcga gcgccttggc ggagtggcgg cgctcaaaga aagcgcacgg
      241 aaaaccaaag ccttcttgcg gggccgggag tttgatgacg ccatctctat gctgcgtgtg
      301 attttggcgt actgcctggg ggatggcggt ttgcggtcta ctgcggcatg ggccgcctcg
      361 attgggctcg ccgatgtcgc caataccgcg ctgctttacc gtctccgcca gagcggagat
      421 tggctcgcct ggctggtagg acaggcgctc gcctccgccg cgcccaaggc gagccaaggc
      481 cgtccgatcc gcatcgtcga cgccactaac gttctaaaag ctggggtcgc cgccaagaca
      541 aaaaataagc tgtggcgcat tcatagcgcg ttcgatctgc ccagtgaacg ctttggtttc
      601 ttcgagctta ccgacgagca tggcggcgag cgactggacc gcgcgcctgt gatcaagggc
      661 gaaatccgct taggcgatcg cgtttacatg cagccggacc ggatagcagc gctgcttgag
      721 gccggcgccg atatcgttgt gaggtccggc tggcggaatg cgcgctggct cgatgccaat
      781 ggcgcccagg tcgatctgct tgccgagttt gagaaagcag cggcgagcgg gcggatcgat
      841 agccccatat gggtcggccg caagtccgct ccagcgctgg ctttgcgtct tgtcgcgatc
      901 aaaaagccgc cccaggccgc cgaagcttct tgccgcgcgg cgcagcgatc cgccaagaag
      961 gcgggaaacg ccatctcgga aggaacgctc accgccgccc actggataat tctcgtaacg
     1021 tctttgcctg ccgatgattt ttcaacagac gatatccttg ccttataccg cctgagatgg
     1081 cggatagaac tcgccttcaa acggctaaaa agccttattg gccttaaggc gcctccggga
     1141 gtggacgagc gctccgccaa gccgtatgtg ctggcccacc ttttggtcat tctcttgctc
     1201 gaaccgctga tcgacgagtt cgaggactct ccccgcctgg ctgtcgccgc ctgacaaggc
     1261 ccggcgcgtg gcgcttgctg ctgcaactca cagccacact tctgcaagcc atcatgccac
     1321 agccctcgct cgccgatttg agccgggcga aagacaagct ccgccgacat ctccacgaac
     1381 cgccgcgacg gcggcgtgta taccagagaa tggcccgctt attttagcgc ctatggggat
     1441 tgatccgggg atcaagggcg accgtgacaa gccaatccgg taccattttc agaagatagc
     1501 cgcctgcgcc gtgattcgat ctatcttccg ggaaagtgca gccgctcagc gagcccacat
     1561 gtcgcaaagg cgacacgtgt tgggattgcg acagcgctcc taatttgaac tttatcgctt
     1621 tatctcaatg ccttaaccgt ttggcacgac tcctgctctg aggggagcaa gatcttcaaa
     1681 cggagcgctc atgcgcgatg catttccaga gttgagacag gatgagggcg gagccgccgg
     1741 agggtttcgg ccatcggccg aaggggggct ctatcctcac cgtcggctga agcgccgcgt
     1801 cgccatcaac gacacgactt tgagggatgg cgaacaaacg cctggcgttg cattcacagc
     1861 caatgaaaaa cttcagatcg ccgcttcgct ctccgatgcg ggcgtcgatg agatcgaagc
     1921 cggcacgccg gcgatgggcg ccgaagagca agacgcaatc cgccggattg cccgtgccgg
     1981 gctcccgatc cgcgtcatgg cctggtgccg catgcggcgc agcgatgtcg atgatgcgat
     2041 cgcatccggc gtcggtattg ttaacctgtc cgcgcccgtt tcacatccgc agatcgtcgc
     2101 gaaattcggc ggctcggtct tgcgcgtcgt tgcggcaacc agagacgtca tcggctatgc
     2161 acgggcgaag ggcctcgaag tcgcgttcgg cggcgaggat tcctctcgag caagccgcga
     2221 gaccctggaa ccgatcctgg aagccgcggc caaggctggt gcgacccgct accgtttcgc
     2281 cgacacgctg ggcgttatcg acccggttgc cacccgcgcg gccatcgaga cggtccggac
     2341 aatgaccgat ctccccatcg agtttcatgc ccacaacgac ctcggatttg caacggccaa
     2401 tacgattgcg gcgattgagg caggggcatc tgccgcgagc gtgacggtaa acgggcttgg
     2461 cgaacgcgcg ggtaacgcgg cgcttgagca ggttgccgtc gcgctcgaca gcatttttgg
     2521 gaatggtcat cgcgtgcatc tccccgcgct caggcacctc agcgcgctcg ttgcgcgcag
     2581 cagcggcatc gaagtgggcc gcgcccagcc cgtcgtcggg aaggacgttt tcacacacga
     2641 atccggcatc cacgtcgacg gcatcctgaa gagccgcgcg tgttacgagg cgctctcacc
     2701 ccagaagctg ggccgcaacc accgcttcgt tctcggaaaa cactcaggtc tgtccgggct
     2761 gcgccacgag ctggcctatc tcggccttca attgacggag gatgaggagc gccagctgct
     2821 gagcgccgtt cgcagctact ccgaacgaca gaagacaacc atccctgcct tcactcttct
     2881 ccgccttgca acctcgctca tcgacgagag aagcgacgac ggcttccggc actgcggtca
     2941 ccaggctgaa accgaaagga acgcggcatg agcaaggtta tctccgacct cgagaagctt
     3001 cacgcggccg aagacttctt ttcctacctt aagctggact acgaccccga agtgcttcgc
     3061 gtttgccgcc ttcatatcct gaagcgcatg ggaaaatatt tgggtggcat ggatttcacc
     3121 ggcgcatccg aggacctgat ttttacaagc gcacaggaaa cgttggccca cgcctatcag
     3181 gacttcgtc
//
LOCUS       DATMGX010000045         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_403907, whole genome shotgun sequence.
ACCESSION   DATMGX010000045 DATMGX010000000
VERSION     DATMGX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403907"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VNR65_00745"
     CDS             <1..590
                     /locus_tag="VNR65_00745"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HWJ17231.1"
                     /translation="WLVKQDPKALPAPFSRFDVVAIASSAIALAAWISAPASLVTAAA
                     CGIAGVLHLIRLARWRGYRTAAEPLVTILHVGYLFVPVGFLLMALAIAFPAVVAPVAA
                     VHAWTAGAIGIMTLAVMTRASLGHTGHELKAAPSTLAIYACALIGALARIAAAFGIEP
                     QIMLHLAATAWILAFGGFAVLFGPLLLSNAKTKLG"
     gene            934..1401
                     /locus_tag="VNR65_00750"
     CDS             934..1401
                     /locus_tag="VNR65_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWJ17232.1"
                     /translation="MLTQQPFSSTFRGVAAAFLFAFLPTHAALANETPPVLSGAKLIT
                     AEEAVKAIGGGAVPVDTRVASEYAEGHIKGALSVPYREKSAKSVDFDASKDQFDLSKL
                     PVNKEASIVVYCNGPECWKSYKAATVSIKGGYSNVHWYRLGFPDWKSKGLPVE"
     gene            1489..>2046
                     /locus_tag="VNR65_00755"
     CDS             1489..>2046
                     /locus_tag="VNR65_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HWJ17233.1"
                     /translation="MMNKFSIATRMMIVFAVMAATQAGITAIAFHGLRLSDHDIAEFY
                     HERLVPMNELSRMNDLMHGSMEQLIIAVIARPSPSNVLPYIDRVKKNLSEMGGLAAQY
                     IKNASGDADKALFADWTSKRDALVLKAIKPAIAALQKQDFNEAEDTVLGVAVKQFAAA
                     QGAFDAVIADALSRAEQTHEQAEDRT"
BASE COUNT          416 a          606 c          574 g          450 t
ORIGIN      
        1 actggctcgt caagcaggac cccaaagcgt tgccagcacc gttcagccgt ttcgatgtcg
       61 tggcgattgc gtcgtcggct attgccctgg cggcctggat atctgccccc gccagcctgg
      121 tgacggctgc cgcttgcggt atcgcaggag tcttgcatct catccgtctc gcacgatgga
      181 gaggctaccg caccgctgcc gagcccttgg ttacaattct gcacgttggc tacctgtttg
      241 tgcccgtggg cttcctgctg atggctctgg cgatcgcgtt tcccgccgtt gttgcgccgg
      301 tcgcggctgt tcatgcatgg acggcgggcg caatcggcat catgacgctc gccgtgatga
      361 cgcgcgccag cctcggccat accgggcacg agctgaaagc cgcgccatcg accctggcaa
      421 tctatgcctg cgcccttatt ggcgctctcg cgcggatcgc ggctgcattc gggatcgaac
      481 cccagatcat gctgcacctc gccgccaccg cctggatact tgctttcggc ggcttcgccg
      541 tgctgttcgg tccgcttctg ctaagcaacg cgaagacgaa gctaggctga gtttcccggc
      601 ttcgcccttc caggttctca gcccctgatg aatttccacg cttaaggcgg tggctgccgt
      661 tttgcccatc aagcggcacg tctgctgtcg gaggtcatgc cgaagtggat ttggtcgtcg
      721 ccatccgcgc ccttggctcg cgattgtgtt ccgccacgat ctccgcaccg gccacaagcg
      781 gctgtcgcag cacagcaatg ccttggttta aggctctggc gagcaagtca tactgcctcg
      841 ttaacgacgc atctaagata tccttgtatg tatttattca tctgtagcgc agatataata
      901 actatggctc agcttaactt tgaaggctcg gccatgctaa cccaacaacc cttttcaagc
      961 acattcaggg gcgtcgctgc ggcgttcttg ttcgctttcc ttcccaccca cgccgcgctg
     1021 gcgaacgaaa cgccgccggt gttatcgggg gcgaagctca tcactgcgga agaagcggtg
     1081 aaagcgatcg gcgggggagc cgtcccggtc gatacacgcg tcgccagtga atatgcggag
     1141 gggcacataa agggtgcact cagcgttccg taccgcgaaa agagcgcgaa atccgtcgat
     1201 tttgacgcaa gtaaagacca gttcgatttg agcaagttac ccgtgaataa agaggcctcg
     1261 atcgttgttt attgcaatgg gcccgagtgc tggaagagtt acaaagcagc taccgtttcg
     1321 attaagggtg gatattcaaa cgtccattgg taccggttag gctttcccga ttggaagtcg
     1381 aaaggcttgc cggtcgaata ggacgccaat actgcgtcgc aatgagcgat ggcgtagctt
     1441 ctcacaagat tgattatcaa gaaaaacgaa gccttggccg agagctcgat gatgaacaag
     1501 ttcagcattg cgacccgcat gatgattgtc tttgctgtga tggctgctac ccaagccggc
     1561 atcacggcaa tcgcttttca tggactccgt ctctcagatc acgacatcgc cgagttctat
     1621 cacgagcgcc ttgttccaat gaacgagctg tcccgcatga acgacctcat gcacggtagc
     1681 atggagcaat tgatcatagc cgtcatcgcc cgcccaagcc cctcgaacgt cttgccgtat
     1741 atcgatcgcg tcaaaaaaaa tctgagcgaa atgggcggcc ttgccgcgca atatatcaag
     1801 aacgctagcg gggatgccga caaggcgctc ttcgcggact ggacctccaa gagggatgca
     1861 cttgtcttga aagccatcaa gccggccatc gccgccttgc agaaacagga cttcaacgaa
     1921 gcggaggaca cggttcttgg cgttgccgtc aaacagtttg cagccgccca aggtgctttc
     1981 gacgccgtca tcgccgatgc gcttagccgc gccgaacaga cgcacgagca ggcggaggat
     2041 cgtaca
//
LOCUS       DATMGX010000046         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_284054, whole genome shotgun sequence.
ACCESSION   DATMGX010000046 DATMGX010000000
VERSION     DATMGX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284054"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..761
                     /locus_tag="VNR65_00760"
     CDS             159..761
                     /locus_tag="VNR65_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17234.1"
                     /translation="MSLMSTERRLFGELALSDENAKRIFDFCPEFYSAIYDDTGAIIA
                     YSSAFPLKPEHASDLITGVMTEPDLEPNMLISAEGSIEKSKVYIGSVVVADRFDLFTR
                     SILLSSLLSWRVTQMTQLAVSRLSVFMVTVSEEGDRMVHFVGAKKLNDGAERKDGKSV
                     YGRTITPGFIARASRSLERCANGRLVEMRFERCHKILAGA"
     gene            complement(914..2110)
                     /locus_tag="VNR65_00765"
                     /pseudo
     CDS             complement(914..2110)
                     /locus_tag="VNR65_00765"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2147..2344)
                     /locus_tag="VNR65_00770"
     CDS             complement(2147..2344)
                     /locus_tag="VNR65_00770"
                     /inference="COORDINATES: protein motif:HMM:NF040425.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
                     /protein_id="HWJ17235.1"
                     /translation="MNRDAPVEPSREELAALIAAQAAEIAALRAHIAELERRLGLNSS
                     NSGKPPSSDGLKKPPRVASLR"
BASE COUNT          474 a          693 c          728 g          510 t
ORIGIN      
        1 ggcgcggctc gagccgcttc tgaaacgacg ccgggagcag tcaattgaaa agcaagacaa
       61 cttgcgttga aatgacaaac caacgtaaaa aacaggctag ggtcgttgag ggtgatgtga
      121 tgtttgtttg tgatgtcccg agaagcttag cagatttttt gagcctaatg tctacggagc
      181 gtcgtttatt cggagagttg gcgctctccg atgagaacgc aaagagaatt ttcgatttct
      241 gtcccgaatt ctattccgcg atctacgacg atactggcgc tattattgcc tattcgagtg
      301 cgtttccgtt gaagcccgaa catgcttcag accttatcac gggcgttatg accgaacccg
      361 atctcgaacc taacatgtta atcagcgcag aaggctcgat cgaaaaatca aaagtctata
      421 tcggctcggt cgttgtcgcc gaccgctttg atctctttac gcggtcgatc ctgctttcca
      481 gccttctttc gtggcgcgtg acgcaaatga cgcagcttgc cgttagccgt ctctcagtct
      541 tcatggtgac ggtttccgag gaaggcgatc gaatggtcca ttttgtcggc gcgaagaaat
      601 tgaacgacgg ggccgagcgg aaagacggca agtcagtcta tgggcgtacg ataactcccg
      661 gattcattgc acgagcaagc cgttcgctgg agcgatgtgc aaatggccgg ttagtcgaaa
      721 tgagattcga gcgttgccat aaaatcttgg caggagcttg agcgttccga gatagcggcg
      781 agggttctgg tgcaaacggc aaaagcgcgg catatttggc ctaatcgcgc cctttagagc
      841 ggaatccgat caacctgtat cgtatccgca ggcattgaag taattttgta actgcctagg
      901 ttcttgagca ggatcacgcc cggcgcaggg ttttcgcgag attttcggga tcggcggtca
      961 aggcgtcgag gattttccac ccctgttttt tggcggtcga gaggaaggaa cggatcgtgg
     1021 cgaagtcgct cgcgccctcc agtgagcgga agccgccgga gattttctga cgcagcttca
     1081 tcatgcggcc gtcgcgttcg gcctgattgt tggtgaaggg cacggctgga tcgtgcagga
     1141 agcgcagcgt atccgccttg cgcgtttcga accgcaacag cagattgtgt ccggttcggc
     1201 gcggcgtgcg gccgcgacgt ttgccggcgc cctttttcgc cagcggtggc tgggcttcgt
     1261 ggaaggccag gccttcggcg acgatggcgt cgtaacggcg ctcgaaacat tcgacgagtc
     1321 gaggcctcaa gggcgcgtcc cgttctctgg cgagattggc ggcgtgacag gcgcggcgca
     1381 acaaccgctg catcttgcgc gcccagtcct ccttctcgat ttcgacaagc gccttcaatt
     1441 cgcgaagatg atgcgcgttg cacagcgcgt gcagcacacc ttgcatcgtg tagtagggtt
     1501 tccagtgatc atgcacaacg acgcccacga cgccgacgat atcggacaag agactgccgc
     1561 gcttggcgca gacgcggtag aaggtcagca acgccgtgca ggccacatgc agccattgtg
     1621 tcttgccgcc gatacgaaag ccggtctcgt ccatgtgctt gaccggcgcg cccgccacgc
     1681 gattgcgtac cgtttcgacg aaatccgcga gccgcttggc gcagccccgg ctcatcctgg
     1741 cgatggtggc agcgaccagc ttcacgccaa agaggtcgcg catcagctcg gccagccgat
     1801 cctcgggcag caattgatag tgcaacagat agagcacgaa cgccgtgatc cgctggccgt
     1861 actgcaccgg cgcattgacg cctgcgggaa aggcggcccg cgtccgcgcg ccgcaagtcg
     1921 cgcactggca gtcgtgggcg cgatgttcgg taacgacggg aggctgcggc tcgggcagat
     1981 cgaacacctg acgggcgctg tggcccgtgg ccatggccgg cgtcagcgtc gaaccgcact
     2041 ccgcgcaagc ctgcggaaaa tgatcgatga tgatgtcggg ttccgcgacc tgccgcaggg
     2101 tctccccgtc atgacctttc tgtccgccgg gcttgttgcc gcttcgctag cgcaggctcg
     2161 ccacgcgcgg cggtttcttc agcccgtcac tcgacggcgg cttgccgctg ttggaactgt
     2221 tcaatccaag ccggcgttcg agttcggcaa tatgagcccg caatgccgcg atttcggccg
     2281 cttgcgccgc aattagcgca gcaagctctt ccctcgatgg ctcgaccggc gcgtcgcgat
     2341 tcatcgaatc cttgaatcgg aactcgcccc cgccgtccac aaaaatctac gcgcccaaca
     2401 aatcc
//
LOCUS       DATMGX010000047         3730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_403356, whole genome shotgun sequence.
ACCESSION   DATMGX010000047 DATMGX010000000
VERSION     DATMGX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403356"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2134..2712
                     /locus_tag="VNR65_00775"
     CDS             2134..2712
                     /locus_tag="VNR65_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17236.1"
                     /translation="MLRILSAHTPYLARWFIGFVAAVRQPNVGAVSDVRLRNLATIKT
                     SMTNECKYCATHTSIYGQAIGVSEAQLEMMKGDAWRDSPLFSEREKATIAWAEAVTRN
                     TAKSDKALWDTMKRLFSDAEIVEITMASAMFNMINRLNDTFWTELETVEYNRRQGNAV
                     KGVTVTDIESFASGFAAAGEAQRRGDSAVAAE"
     gene            complement(2986..>3730)
                     /locus_tag="VNR65_00780"
     CDS             complement(2986..>3730)
                     /locus_tag="VNR65_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HWJ17237.1"
                     /translation="IGEVVRAKPDGYTILANSSAQTIAPWIVPNFPYDVAKDLSGALM
                     IGQNSNVLLVIPSKGWKTAQDLIAAAKAKPSTINYGSAGVGTATHISAVRFSLAAGIQ
                     TTHVPYKGGAEALTDLLGGRIDFLFTPISTALPLIRDGRVVPLMVSTATRASDLPNVP
                     TPGDLGFVNAETTVWYAVFMPAKTPRDIVEKFHDVGMKVLATPEMRAKLKKLAVDPMP
                     MTTAEMDKFVASELANNGKLIKAAGISPK"
BASE COUNT          748 a         1050 c         1109 g          823 t
ORIGIN      
        1 gccgccaatt tcgtcattgg cgcgtttgtc cgctattggc acttagcgtc attccgcttc
       61 gccggaaaat tcggtcgcta ttggactaac agcggacatt gatcggcgcg ggcgctgaac
      121 ggctcggtcg tgaatgaccc aaagcggaca tcggcattca cgccctaatc actgacactg
      181 aatcatgacg ccgttcgggc ccatatgcgg accacggcaa ggaggctgag tgtgacccgg
      241 cttacaaggg tgggaggatt tggtacaatc agtagatctg ggcgggctgc cgccagcgcc
      301 tgagccggag ggtgcggcgg aagtgccgga cgtcttcacc tgacttgcgc cacttgaacc
      361 gcttgtcttc gcgaaggcgt gactcccgct cagggtcaac gccactgaga tcgcgacgag
      421 cgacattgct ttcatggctg ctttccttat tccttttcca atcactgaca ctgaatcatc
      481 accccgttcg gacccatatg tcctccacgg caggtaggct gagtggtgcc gggccgaggt
      541 gctacgatgc atttggggcc agtgccgcca ttatgacacg catgtttttg ggactgcccg
      601 ccgccagcgc ttgagctgga ggtagaagtg ccggaagtct tgacctgact tgggccgccg
      661 ccactcttcg cgaaggcgga actaccactc aacgtcaatg ccactgaaat cgcgaggaac
      721 gaaacggcct tcgagattgc tgtcatagct gtttcctcta tttgtagccg atcagtcgca
      781 ctgaatcatg acaccgttag gtcccttatg cggtccctta catgtcggtt gtgtggtccc
      841 gcctggtaca gacatgcaat attggccacc acccctctct ggatgacaga ctatgacacg
      901 cggtggcgta acatgcttgc tgcgggtgct ggtggagccc gagggagcgg cagaagtgcc
      961 ggatgtcttt acctgtcccg gaccgacggt cttcgcaatg acgaggctac cgctcaacgt
     1021 caatgcagcc gaaatcgcga ggagtaaaac gactttcaac attgctgtca taaccgtttc
     1081 ctcttgctgt tctcgatcag tcacactgaa atatgacgtc gggaacgtgc ctctcgcttc
     1141 ttaggtcgtc actgcgccga gaatggttca aaccatgcag cgttattaat ttcgtttgct
     1201 ccgaatctgc gccggcggtt gggtaggcgc gttcggattc gaaaccgttg tcggtgggtt
     1261 tttcaacttg ccaggatcaa ctttacgggg cgggggatcg ggcgagtttg gccttccgat
     1321 ggactgcggg ttttttccgg acccccaata tttgttacat tgcgtagtgc actttaggtt
     1381 actcgcatcc gttttgttat gctcattgca aaagtcaata catttctgaa accctttttg
     1441 acacgtatca tcacatttcc agcgtgcttc ggcgacgaag gtaagcgaag caagaaatgc
     1501 gactgacaga aacgccagtg aaagaaccac tggcaatttg atccgcaatt tcatcatagc
     1561 gtcgcctctc ttgttgctga caccaaacta taaatcttat tgaacgcccg tatttatggc
     1621 aatgcggctt tggccaagag tgacttgcgc acagaacgct gctgcggctg gtctgaatgg
     1681 atttcaaacg taggtcgcga gtgattttga ccgcgttgca atgggtggtc gagtactgac
     1741 gctatttgct gcggtgcgtt tgtccgcttt tggcacatcg cgtcattcgc ggcgctgcaa
     1801 catacttggt cgctattggg cgtacagcgg acatcgatag gaacgggcgc tggagctgaa
     1861 agggtcgccg ctaataaccc gtaccggaca tgaacggccg attttgctgc gccgcactgc
     1921 gcgatgacgt gctagcctaa ggtcgttggc cctgggagaa gcccatgagg tgacccgaag
     1981 ggattcattc gggaaactcg gaccgacggc ataagtcagg gaaggaaacc gacgatgaca
     2041 cagcgcttgc ccggaatttc cgatgcagac gccactggcg tcgccaagga tacgctcgcc
     2101 ggttcgacca aactgctcgg ccgctcgtcc aatttgctgc gcatcctgtc ggcgcacacg
     2161 ccctatctcg cgcgctggtt catcggtttc gtcgcggcgg tgcgccagcc caatgttggg
     2221 gcggtgtcgg acgtgcggtt gcgcaatctc gccaccatca agacctcgat gaccaacgag
     2281 tgcaaatatt gcgcgacaca tacttcgatc tacgggcaag cgattggcgt cagcgaggcg
     2341 cagctcgaaa tgatgaaggg cgatgcctgg cgcgacagcc cgttgttcag cgagcgcgag
     2401 aaggcgacca ttgcctgggc cgaagcggtg acacgcaaca ccgcgaaaag cgacaaggcg
     2461 ctgtgggaca ccatgaagcg cctgttcagc gacgccgaga ttgttgagat tactatggcg
     2521 tcggcgatgt tcaacatgat caaccggctc aacgacacgt tttggaccga gcttgaaacc
     2581 gtcgaataca accgccgtca gggcaacgcc gtcaaaggcg tcaccgttac cgacattgag
     2641 agcttcgcca gcggctttgc cgccgccggc gaggcgcagc gccgcggcga tagcgcggtg
     2701 gccgcggagt gacgcctcga taaacgattt gctgcggtga atcggtcgcg attggcactt
     2761 agcgtcatgc gccgcaccgc aatatatttg gacgctattg ggggcatagc cgacattggt
     2821 cggcgctggc gcgaaatggt tatgaagcta atgaccgata acgtcacctt ttaaacagac
     2881 caagcgacgt taggctcatc gagtgctctt tgtcccgtct ctggccccat tgttgctgga
     2941 ccttggaacc gtgccgccgc aaccctctgt actgcgagag ggagatcact tcgggctgat
     3001 gcctgccgct ttgatcagct tgccattgtt ggcaagctcg ctggcgacga acttgtccat
     3061 ctcggccgtt gtcatcggca taggatcgac cgcgagcttc ttcagcttcg ctctcatctc
     3121 cggcgtcgcc agcactttca tgcccacgtc atggaatttc tcaacgatgt cacgcggcgt
     3181 cttcgccggc atgaacactg cgtaccagac cgtggtctct gcattgacga agccgagatc
     3241 gcccggcgtt ggcacgttgg gcaggtcgga ggcgcgcgtc gctgtcgaca ccatcagcgg
     3301 aacgacgcgg ccgtcgcgga tcagcggcag cgcggtcgag atcggcgtga atagaaaatc
     3361 gatgcggccg ccaagcaaat cggtcagcgc ctcggcgccg cctttgtacg gcacgtgcgt
     3421 cgtctgaatg ccggcggcga ggctgaagcg cacggcgctg atatgggtcg cggtgccgac
     3481 gccggccgag ccgtagttga tggtactagg cttggccttg gccgcggcga tgagatcttg
     3541 cgccgtcttc cagcccttcg acggaatgac gagcaacacg ttagaattct ggccgatcat
     3601 cagcgcgccc gaaaggtcct tggccacgtc ataagggaag ttcggcacga tccacggtgc
     3661 gatggtttgc gcggaggagt tggcgaggat tgtgtagccg tcgggcttgg cgcgcacgac
     3721 ctcgccgatg
//
LOCUS       DATMGX010000048         3019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_469841, whole genome shotgun sequence.
ACCESSION   DATMGX010000048 DATMGX010000000
VERSION     DATMGX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469841"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VNR65_00785"
     CDS             <1..314
                     /locus_tag="VNR65_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ17238.1"
                     /translation="VLVSVACNGAHSLKQRLARWLLMMRDRCDDDALLITQDLLAEML
                     GVQRPTITNAARELERAGLIARGRRQVTILDRQGLVGASCECYQLVRARLASHLPKTY
                     A"
     gene            491..679
                     /locus_tag="VNR65_00790"
     CDS             491..679
                     /locus_tag="VNR65_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17239.1"
                     /translation="MRELLPLREVNPLDLVETIREGILVLKPDLTIRFANRSFCHTFA
                     LLVAGFSNGTKLLLRVCN"
     gene            complement(687..1682)
                     /locus_tag="VNR65_00795"
     CDS             complement(687..1682)
                     /locus_tag="VNR65_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17240.1"
                     /translation="MSLLSSHGCAGSDASAPITKSCSTVESTLAEMPPVDHRISQSFK
                     CVVQLAQAFEPQQQTPELILPSEHALDGVETLFKNGGVEQRLAATLGYFSTAWIRVDV
                     RGHPAIENRFAINPAIVDAIQADNGSFKIKAYSIRYARHKRQGFPQERRFIAIARRRN
                     KRRDHGATPIAENDDLVTLHFFVTTEPDVVAAFLRGCRRSIAMNDGCVEKIGVMKRRH
                     RVSKNGVKAAIGLPLSKRAIDARVMNFRTTVSISFDRQFFPLAPQIEHLQNVIEDRMH
                     REFWRRASASGGKMGQDKLLELLNAQFLRNRPPFLAFRHFSSQINWNISRVGRYP"
     gene            1828..2169
                     /locus_tag="VNR65_00800"
     CDS             1828..2169
                     /locus_tag="VNR65_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWJ17241.1"
                     /translation="MFLLREELRRQRSITCDEAMQARDGRWVEVAGLVLMRQRPGTAK
                     GVMFITIEDETGIANLIVWPSVYEKQRRIILSASMLGVRGRLQREGEIVHIIAHWLTD
                     MSGNLAKIGAA"
     gene            2271..2912
                     /locus_tag="VNR65_00805"
     CDS             2271..2912
                     /locus_tag="VNR65_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWJ17242.1"
                     /translation="MSDTIIETAAPEGAATCHDVEVALRGRGSASMRFMGWNMTEADL
                     NGLDLQGCEFVRCRAGHANFSSCDFTEARFLFCDFNNSKWRGTIVSSAFFQDCKLTGA
                     QMAVANTLMSPAFERCLLINANLRGLSFRRGQLDGVDFQGADLREADFRDAVLTGCSL
                     REANVTNARFEGADLRGADLGSLQLADASRFKGAIISKKQAGELLSGLGLKVV"
BASE COUNT          646 a          842 c          862 g          669 t
ORIGIN      
        1 aggtcctggt gtcggtagcg tgcaatggcg cgcacagcct caagcagcgg ctggcgcgtt
       61 ggctgctcat gatgcgtgac cgctgtgacg atgacgcact gttgataact caggacctgc
      121 tcgccgaaat gctgggtgtc cagaggccaa ccatcacgaa tgctgcccgg gagttggaac
      181 gtgccggttt gattgcgcgc ggccgacggc aagtcacgat acttgatcgg cagggcctcg
      241 ttggggcatc ttgtgaatgt tatcagttgg tccgggcgcg cctcgcttcg caccttccca
      301 aaacatacgc ctaaaaaagc gagctcccac cacgtcggta atatacatta ccgacaacgg
      361 cccctcggta atatacatta ctgagaagcc ccctcattga gcttacaagc aagactggtt
      421 ggaggccgac gtatgctctc gagcaagatc gcattcttgc tgtcgaggct gcgtcccgtc
      481 ttcagaagct atgagggagc tgctgccatt gcgcgaagtc aacccactgg atctcgtgga
      541 gacgatccgc gaaggcatcc tcgtgctcaa gcccgatctg accatccgct ttgcgaaccg
      601 ctccttctgc cacacattcg cgctactggt agcgggtttt tccaacggca caaagctgtt
      661 attaagagtt tgtaattgat cacattttaa gggtagcggc cgacgcgtga aatattccaa
      721 tttatttgcg aggaaaaatg acgaaaagcc agaaacggcg gtcgattccg aagaaattga
      781 gcattgagca gttccagcaa tttgtcttgc cccatcttac cgcccgaagc cgaggccctg
      841 cgccaaaact ccctatgcat tcggtcttca attacatttt gcaggtgctc tatctggggt
      901 gccaatggaa agaactgccg atcaaaagag atcgagacgg tcgtccggaa attcattaca
      961 cgcgcatcta tagcgcgttt cgaaagtggc aggccgatgg ctgctttgac gccatttttg
     1021 ctgactcggt gtcgacgctt catcacgccg atcttctcga cacagccgtc attcatggcg
     1081 atggaacgac gacagccgcg aagaaaggcg gcgacaacat cgggttcagt ggtcacaaaa
     1141 aaatgaaggg tgacaagatc gtcgttttct gcgatcggcg ttgcaccgtg atctctccgt
     1201 ttgtttctgc gccgggcaat cgcaatgaat ctccgctctt gcggaaagcc ttgccgcttg
     1261 tggcgtgcat agcggatgct gtaggccttg atcttaaagg aaccattgtc agcctggatg
     1321 gcgtctacga ttgccggatt aatcgcaaat cgattttcaa tcgcgggatg accccgaaca
     1381 tcaacccgaa tccacgcggt agaaaaatag ccaagcgtgg ccgcaagccg ctgttcgacg
     1441 ccgccatttt taaagagcgt ttccacacca tcgagcgcgt gttcggatgg gaggataagt
     1501 tccggcgttt gctgctgcgg ttcgaacgcc tgagcgagtt gcactacgca tttaaagctt
     1561 tggcttatac gatgatcaac cggcggcatt tctgccaggg ttgattcaac agtcgaacag
     1621 cttttcgtga tcggcgcgct tgcgtccgaa cctgcacatc cgtgcgacga caaaagcgac
     1681 attcaatgcg tcacatttct caaaactcgg cgcgtcgacg tcccatctta cgcaatttcg
     1741 ttttctcgat cgctttttca aacgccgaac atggaaaccc gccaccagta gtatggccat
     1801 gtcgggctgt cgctccgcaa tcatccggtg ttcttgctgc gcgaagagct tcggcgccag
     1861 cgcagcatca cctgcgatga agccatgcag gcgcgggacg ggcgttgggt ggaggttgcc
     1921 ggccttgtcc tgatgcgcca gagaccgggc acggccaagg gcgtcatgtt cattacgatc
     1981 gaggacgaga ccggcatcgc caatctcatc gtctggccct cagtctatga gaagcaacgc
     2041 cgcatcatcc tttccgccag catgctgggg gtccgcggac ggcttcagcg cgaaggcgag
     2101 atcgtccata taatcgcgca ctggctgacg gatatgtccg gaaacttggc gaaaataggc
     2161 gccgcctgaa cttgcaatca gcaacccgat tcgaaaacat gcgagcgaaa atcgccgtcc
     2221 tcagccggga tcgcggacat gaagccaccg tcgttttgca ggaacgctca atgagcgaca
     2281 caatcatcga aactgccgcg ccagagggag cggccacctg tcatgacgtc gaggtggcgc
     2341 tgcgtgggcg tgggtcagcg tcaatgcggt ttatgggatg gaatatgacg gaggccgacc
     2401 tcaatggact cgacttgcaa ggatgcgaat tcgtgcgctg ccgcgcgggc cacgccaatt
     2461 tttcatcctg cgatttcacc gaggctcgat tcctgttctg cgatttcaac aactccaaat
     2521 ggcgcgggac aatcgtatcg tccgccttct tccaggattg caagctgacg ggcgcccaga
     2581 tggcggtcgc gaacacgctc atgtcgccgg cgttcgagag atgtctgctg atcaacgcga
     2641 atctgcgtgg tctgtcgttc cgacgagggc aattggacgg cgttgacttc cagggcgccg
     2701 atctccgaga agccgatttc cgggacgccg tgttgaccgg ttgcagcctt agggaggcga
     2761 acgtgacgaa cgcgcgcttt gaaggggccg acttgcgcgg cgccgatctt ggcagtctcc
     2821 agcttgccga cgcgtcgcgg ttcaaaggcg cgatcatttc caagaaacag gccggggaac
     2881 tccttagcgg ccttgggctg aaggtcgttt gacgtccact cgtccttgag attccgggtc
     2941 agtccatgtg ctggtcatgg tcgccgatcc agagtcgtga agtcgtccct tagtttggcg
     3001 gttccgcaat gcgcggttg
//
LOCUS       DATMGX010000049         3330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_403197, whole genome shotgun sequence.
ACCESSION   DATMGX010000049 DATMGX010000000
VERSION     DATMGX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403197"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..571)
                     /locus_tag="VNR65_00810"
     CDS             complement(86..571)
                     /locus_tag="VNR65_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17243.1"
                     /translation="MILLVSTSSRAKECAAALEQGTGHPTQVANSVPHAVTRLQTTEF
                     DALAIDQSLLEADFRALDTLLNHAGTAQPIYVNLALHHNDRVVREAQVALRRAEKEKQ
                     WAMGMAERNLRNQLRGEVTGILLTSELALRQPAIPAEVVAKIRLVQDMAEKMRSRLHV
                     F"
     gene            complement(712..2193)
                     /locus_tag="VNR65_00815"
     CDS             complement(712..2193)
                     /locus_tag="VNR65_00815"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWJ17244.1"
                     /translation="MSTKAQINSAPPLGDGLAEEGMSHVHAVETMAVSETSCEAKGLN
                     LLIVDDERSMRDSCQEVAHSLGFNSQVADTPEHAYQVLETSSTDVVLLDLRLPGNSGL
                     EVLREIKQRHPETLVIVMTGFATVQSAVQAMKLGAYDYVTKPFNFEELRLLLERASAH
                     LKLTAENRLLREQVKSKHGFGSLVGHAPEMEKLYRIIAKAAHSTHPVLILGESGTGKE
                     LVARSIHYSGPFRDCPFIPVDCGSLVPTLIESELFGYVRGAFTGAVRAKDGLLATAEG
                     GTVFLDEVGELPVDLQAKLLRAIQEKEVRPVGGTRAIPINVRILAATNRDLDAAVQQG
                     TFRRDLYFRLNVLTLRIPPLRERKQDIPLLIGNALERINGATEMQHSVGDDALRAMLN
                     YDWPGNVRELENCVERACTTCSLPTIHIADLPTQLRNFQAQAKPAGPVSDREIVTPIA
                     ELEKQAILHAIEALQGDKLEAARQLGIGKTTLYRKLKEYGAAL"
     gene            complement(2199..2774)
                     /locus_tag="VNR65_00820"
     CDS             complement(2199..2774)
                     /locus_tag="VNR65_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17245.1"
                     /translation="MATHNPIGSVTQMQPLANPLDRFPIAGNDETSQMSSAGQGREEC
                     DPGNRRSSRPFSGDCADLVHELANTTTAVLMNAQVLGWKLPPYSRLKRPVREIERNAQ
                     RGGELMRRLLSRLATGATGPASEDGPCVPAPGLGLTNTVTAQEPGVDSRSAANLPPRT
                     SSHPAPGFSSAAEVELTARCDGCTSMFPKKG"
BASE COUNT          592 a         1038 c         1020 g          680 t
ORIGIN      
        1 gcgtaactcg tagagagcgc ccgccgcgac gccaatcacg tcacggaaat cttcggttca
       61 aaaaccgggg ttcccggatg ggtttctaga agacgtgcag ccgcgagcgc atcttctcgg
      121 ccatgtcctg caccaggcga atcttggcca ccacctcggc ggggatggcc ggctgccgca
      181 acgccagctc cgaggtgagc aggatgccgg tcacttcgcc acgcaactgg ttgcgcaggt
      241 tccgctccgc catgcccatg gcccattgct tttctttctc cgcccggcgc agggcgacct
      301 gcgcctcgcg caccacccgg tcgttatggt gcagcgccag gttgacgtaa atcggctggg
      361 cggtcccggc gtgattcagc agagtgtcca gcgcgcggaa atcggcctcc agcagcgatt
      421 gatcgatcgc cagggcgtcg aattccgtgg tctgcagccg cgtaaccgcg tgcggtaccg
      481 agttggccac ctgggtgggg tgtcccgtcc cctgttccag cgcggcagcg cattccttgg
      541 cgcgagacga tgtgctcacc agcaggatca tacattcctc cctgccgccc tttcgccggg
      601 cacacttcgc cagtgacgcc ggcgggcggc ggtgatcggt ccaacaacca tggtccggcg
      661 cttacttcga tcgcccccta cgcgccgcat tggcgcgcag aacgacgacc atcagagcgc
      721 cgcgccgtat tccttcagtt tgcgatagag cgtggtcttg ccgatgccca gctggcgcgc
      781 ggcttccagc ttgtcgccct gcagcgcttc gatggcgtgc aggatggctt gcttctccaa
      841 ctcggcgatg ggggtgacga tctcccgatc ggagaccggg cccgccggct tggcttgtgc
      901 ctggaagttg cgcaactgcg tcggcagatc ggcaatgtga atggtgggaa gagagcaggt
      961 cgtgcaggcg cgttccacgc aattctccag ttcgcgcacg ttgcccggcc aatcgtagtt
     1021 gagcatcgcc cgcagggcat cgtcccccac gctgtgctgc atctcggtcg cgccgttgat
     1081 ccgttccagc gcgttgccga tcagcagggg aatgtcctgc ttgcgttcgc gcagcggagg
     1141 gatgcgcaag gtaagcacgt tcaaacggaa gtagaggtcg cggcggaagg tgccctgctg
     1201 caccgccgca tccaggtcgc ggttggtggc cgccaggata cgcacgttga tgggtatggc
     1261 gcgggtaccg cccaccggac gcacttcttt ttcctggatg gcgcgcaaca gcttggcttg
     1321 caggtccacc ggcagctcgc ccacttcatc gagaaagacc gtcccgccct cggcggtagc
     1381 cagtagtccg tctttggccc gcaccgcgcc ggtgaaggcg ccgcgcacat agccgaacaa
     1441 ctcgctctcg atcagggtcg gaaccaggga gccgcaatcc acgggaataa agggacagtc
     1501 gcggaagggc ccggagtagt gaatggagcg tgccaccagc tccttgccgg tcccactctc
     1561 ccctagaatc agcacgggat gggtgctgtg cgcggccttg gcgataatgc ggtacagctt
     1621 ctccatctcc ggcgcgtgcc ccaccagcga accgaagccg tgcttggact tcacctgttc
     1681 ccgcaacagg cggttctcgg cggtgagctt caggtgggct gaggcccgct ccagcagcag
     1741 gcgcagttcc tcgaagttga acggtttggt aacgtagtca taggccccca gcttcatcgc
     1801 ctgcaccgcc gattgcaccg tggcaaagcc ggtcatgaca atcaccaggg tctcgggatg
     1861 gcgctgcttg atctcgcgca aaacctccag accgctgttg cccggcagac gaagatcgag
     1921 cagcacgaca tccgtgctgc tggtttcgag cacctggtag gcgtgttccg gagtatcggc
     1981 cacctggctg ttaaacccca gcgagtgggc gacttcctgg catgaatcgc gcatcgagcg
     2041 ctcatcgtcg acgatcaaca ggttcaggcc tttagcttcg caactggttt cgctaactgc
     2101 cattgtctca acggcatgca cgtgggacat cccttcttcg gccaagccgt ccccaagtgg
     2161 cggcgccgaa ttgatttggg ctttagtact cattaccatc atcccttttt gggaaacatg
     2221 ctagtgcacc catcacatcg tgctgtgagt tcaacttccg cggctgagga aaaaccaggg
     2281 gccgggtggg aactcgtccg gggcggcagg ttcgccgcac tccgggagtc aacgcccggc
     2341 tcctgggccg tcaccgtatt cgtcaacccc aacccggggg ccggcacgca tggcccatct
     2401 tcgcttgccg ggccggtggc gcctgtcgcc aagcggctca acagccgcct catcaattcc
     2461 ccgccacgct gcgcgttgcg ctcgatttcc cgcaccggcc gcttcaagcg gctgtaggga
     2521 ggcagtttcc atcccagcac ctgggcattc atcagcactg cggtcgtggt gttcgccagc
     2581 tcatgcacca ggtcggcgca gtcgccgctg aaggggcgcg aggagcgccg gtttcccgga
     2641 tcgcactcct cgcgcccttg cccggcggag gacatctggc tggtttcgtc gttgcctgcg
     2701 atcggaaagc gatcgagtgg gttcgccaag ggttgcattt gcgtaacgct gccgatcggg
     2761 ttgtgggtgg ccatgttcat gatccagatt cgcttggttt cggattgcaa acgtatctgt
     2821 atacagcacg gctgctgcca aaactccgct cgcgcttaag cgttgattct tttgggaatt
     2881 ttccgaatcg ctacgaatcc gaaaccgcgg ctgccgtcgc cgcagtttca gaatgaaact
     2941 cagtcccgcc ggatataagt ctaagtgccc gtcaaatcag ggtttcgacg gtttcctggt
     3001 tggggaagga aaatgtgcgg aaagatcaaa tcgggaaggg cgggatggcg gttagcactt
     3061 ggccgttggc acttagccgt tagcacttgg cttttagctc ctagctctta gcaattagca
     3121 attagcaact agcaactagc aactagcgtg gataatccgt gacatcggat ggacgcaact
     3181 ctagcgtcaa cgcaggccag aatatggctt ctgcccaaga gcagattctg agttggtgta
     3241 tccgggtgga gcaccgcttc agcggtgcat ttgagagttg catctgaatt cggctttagc
     3301 cgctgaggta accagagctc ttacctcagc
//
LOCUS       DATMGX010000050         4086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_370399, whole genome shotgun sequence.
ACCESSION   DATMGX010000050 DATMGX010000000
VERSION     DATMGX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370399"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VNR65_00825"
     CDS             complement(<1..703)
                     /locus_tag="VNR65_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ17246.1"
                     /translation="MRTPGKAALPAFEYIGSRRSLKALTAIGIFAALLSASSSARAAP
                     DKSLIKSQEGSSALLRGRFDLAVAAYDDALKDTNLPAASQAGLYSDRGVAKWRLKRPD
                     EAIADFTKAVTLNPEYAPAYNNRGNVFLELNRAEDAYKDFDRAVSLAPDFGVAYSNRA
                     NANQRLNKLEAAEKDFRKAVELMPGSAIPLNGRGKIASAAGQYYTALRYLNRAISLNP
                     QYAPAYQNRAAVYASL"
     gene            764..2026
                     /locus_tag="VNR65_00830"
     CDS             764..2026
                     /locus_tag="VNR65_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWJ17247.1"
                     /translation="MGGLALALVLLAAAASAAEYSGATVPGLPWLDVFGNSPPSGEVK
                     TVENGVAPASDDAANAEARKRLKNELDRMRKGTRHEKAESKPNSQQPAKKKEPTPEAA
                     DAPHPQPPKPPVPAVDPEISRAAGKLMIMRFSGTEPSDAGPKAIRGLIHDGQIAGVMF
                     GSDNIQTKIQLKELVKSLGQSDGETKPLLAISEIGGAGGAFPRIKDFESWPSEREVAS
                     KGDPEYAYLTYRSLGTYLTGFGFNLNFGPTLGGASRDPSASFGDSPLQAGVFAKTFIL
                     GHRDDNIATVPVVDSSDVAVRALKTLLVSYPGMAIAVTTASEAQPFAAYDSLVRGPRF
                     CMISLKQGSNPAEAASSFNRGCDAIVIDGGKENPAVIRDLVVRGVSGAIKSGSLSLAT
                     LKASAQRLSALRSPSQSAPAAFTTRTAQ"
     gene            2293..2919
                     /locus_tag="VNR65_00835"
     CDS             2293..2919
                     /locus_tag="VNR65_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ17248.1"
                     /translation="MKRIGLRWTLPAMILALAVPAYAETGEHSKDVEAKIAYCKKCHG
                     QMGQGLAAAYTVPRLAGQTIPYLEAKFGIISEHKRDNVTAETFMVPVLGSVDPAIRKA
                     VATHFNGLDPAPSGGGNKDLIPEGRKLYEAGVPEAGIPSCTNCHGADGKGSEMNPRLA
                     GQVYPYTVKVLTNWAIINKEQEGVKAPVQHTLTDAQITAVASYLSNLK"
     gene            complement(2985..3752)
                     /locus_tag="VNR65_00840"
     CDS             complement(2985..3752)
                     /locus_tag="VNR65_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HWJ17249.1"
                     /translation="MANPAAGRASVAEAFAGTQLWIFDLDNTLYPAECNLFAQIDQRM
                     SAFIQTLLGLDPVAARKVQKDLYYEHGTTLSGLMAKHDVKPDDFMDFVHDIDLAPVSH
                     MPELAEAISRLDGRKFIFTNGSARHAERVAGKLGVLDAFDGIFDIAAGGYVPKPKRES
                     FARFLDFCAGGKCKAAMFEDLPHNLEAAHDLGIVTVLVRSNYYDHPSQHALKEAEALP
                     RYIHFETDNLTHFLNNVVLNTQGAIEPQDHSPVERKG"
     gene            complement(3745..>4086)
                     /gene="argB"
                     /locus_tag="VNR65_00845"
     CDS             complement(3745..>4086)
                     /gene="argB"
                     /locus_tag="VNR65_00845"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWJ17250.1"
                     /translation="RDGTTYNINADTFASALAGALKAKRLLLLTDVAGVLDQNGKLIH
                     RLTVSEAKAMMADGTITGGMIPKIEGCLSVIEQGVEGVVILDGRVHHAVLLELFTEHG
                     VGTLIERDADG"
BASE COUNT          886 a         1189 c         1195 g          816 t
ORIGIN      
        1 tgagagatgc gtaaaccgca gccctgttct gataggcggg ggcatattgc gggttgagcg
       61 aaattgcacg attaaggtag cgaagcgcgg tataatattg acccgccgcg ctcgctattt
      121 tgccccgtcc gttcaggggg atcgcgctgc ccggcataag ctcgacggct ttgcggaaat
      181 ccttttcggc agcctccagc ttgttcagtc tctgattcgc gtttgctctg ttgctgtaag
      241 cgacgccgaa atccggagcc agcgaaaccg cacgatcgaa atccttgtag gcgtcctcgg
      301 cgcggttcag ttccagaaag acgttcccac ggttgttata ggcgggcgca tactcgggat
      361 tgagcgtaac ggccttggtg aaatcggcga tggcctcgtc aggccgcttc aggcgccatt
      421 tggctacgcc gcggtcgctg taaaggccgg cctggctcgc ggcggggaga tttgtatcct
      481 tgagcgcgtc gtcataggcg gcaacggcga ggtcgaatcg gccccgaagc agagcggagc
      541 ttccttcctg ggattttatc agcgatttgt ctggcgcagc ccttgcagaa gaggaagcac
      601 tgagtaacgc agcgaatatt ccaatcgccg tcagcgcctt gaggctccgt cgtgaaccaa
      661 tatactcaaa agcgggtaag gctgctttac cgggcgtccg cattccgcta acacatcgag
      721 aaaaccaccg acgttcctgc caatgcatcg aaaatatccc tttgtgggcg gactggctct
      781 ggcccttgtt ctgcttgccg cagcggcttc cgcggcggag tatagcggcg caactgtgcc
      841 tgggctaccg tggcttgatg tttttgggaa ctctcccccc agcggtgagg tgaaaacggt
      901 cgaaaacggc gtcgctcccg cgagcgatga cgcagccaac gcggaggctc gcaagcgtct
      961 taagaacgaa ctcgatcgca tgcgcaaggg cacccggcac gaaaaggcgg aatcaaagcc
     1021 caactcgcaa cagcccgcga agaagaaaga gccaacgccg gaagctgccg atgcgcccca
     1081 cccgcagcca ccaaagccac ctgtcccggc cgtcgatcct gaaatcagcc gcgccgctgg
     1141 aaaacttatg atcatgcgct tcagcggcac cgagccctcg gacgctggac cgaaagcgat
     1201 tcgcggcctg atccatgacg gacagatcgc cggtgtcatg ttcggcagcg ataacattca
     1261 gacgaaaatc cagctgaagg aactcgtcaa atcccttggg caaagcgacg gcgaaacgaa
     1321 gcctcttctt gcgatcagcg aaatcggcgg cgctggcgga gcatttcccc gtatcaagga
     1381 tttcgaaagc tggccttccg agcgtgaggt ggcgtccaag ggcgatcccg aatatgccta
     1441 cctgacctat cgttctctgg gcacctacct gactggtttc ggcttcaatc tgaatttcgg
     1501 cccgacgctc ggaggtgcct cgcgagaccc gtcagcaagc ttcggcgaca gtccccttca
     1561 ggctggagtg ttcgccaaga cattcatcct tggccacagg gacgacaaca tcgccaccgt
     1621 tcccgttgtc gatagcagcg atgttgccgt tcgagctttg aaaacgctgc ttgtctccta
     1681 tccaggcatg gcgatcgccg tcaccacggc aagcgaagca cagcctttcg cggcgtatga
     1741 cagccttgtg agggggccgc gtttttgcat gatcagcctt aagcagggaa gcaatcccgc
     1801 cgaggcggcc agctctttca atcggggatg cgatgccatc gtgatcgacg ggggaaagga
     1861 aaacccggcc gtcatcaggg accttgttgt tcgcggcgtg tccggcgcaa tcaaaagcgg
     1921 ttcgctgagc cttgcgacgc tcaaagcctc cgcgcaaaga ctttccgcac tccgatcccc
     1981 gtcccagtct gcccccgcag cattcacaac gcggacggcg caatagcaag tcttcgcggc
     2041 agcaaaaccc atttcgtcaa tttttgatgc atcttgtgaa aagctaaggc cgcgttaaag
     2101 agtagcagtg atatattgct gcatctgatg tatctcaatt ttcataaatg atgcgctttg
     2161 agctagaaaa atccgctcac gattttccgg ctcttttatc ttccgaagcc agagcccggc
     2221 tcggaaggtg catggcgcta ccggataaac gtcttttccg gaaacctaaa cgaaggcggg
     2281 gaggcgaaaa caatgaaacg tatcggtttg cgatggacac tgccggcgat gatcctggca
     2341 cttgcagttc cggcatatgc cgaaaccggt gagcattcca aggacgtcga agccaagata
     2401 gcgtactgca agaagtgtca tggccagatg gggcagggcc ttgcagcagc ctataccgtg
     2461 ccacgcctgg cggggcagac gatcccctat ctcgaagcca agttcgggat catttccgag
     2521 cacaagcggg ataacgtcac ggcagaaacg ttcatggttc ctgtgctggg aagcgtcgac
     2581 cccgcgatca ggaaggctgt cgccacacac ttcaacgggc ttgatcctgc gccttcaggt
     2641 ggcggaaata aggacctcat acccgaaggc aggaagctct atgaagctgg ggttcccgaa
     2701 gctggtattc cctcatgcac gaactgtcac ggcgccgacg ggaaaggctc cgagatgaat
     2761 ccgcgcctgg ctggtcaggt ctatccttac accgtcaagg tgctgacaaa ctgggccatc
     2821 atcaacaagg aacaggaagg cgtaaaagca cccgttcagc atacgttgac ggacgcccaa
     2881 attacagcag ttgcgtctta tctcagcaat ctgaaatagc ggaaagcgca atgccgttgc
     2941 ctgcggcgct cgttgcaggc acaccgaaaa ggccgacggg agggttagcc cttccgctcg
     3001 acggggctgt gatcttgcgg ttcaattgca ccttgagtgt tcaaaacgac attatttaaa
     3061 aaatgggtga ggttgtccgt ctcgaagtgg atgtagcgcg gaagcgcttc cgcctccttg
     3121 agggcgtgct gcgaggggtg atcgtaatag tttgagcgga caagcacggt gacgatgccc
     3181 agatcgtgcg ccgcttcaag attgtgcggc agatcctcga acatggcggc cttgcacttt
     3241 ccgcccgcac aaaaatcgag aaagcgagcg aaggactcgc gcttcggctt cggcacgtag
     3301 cctcccgccg cgatgtcgaa gatgccgtcg aacgcgtcga gcacgccaag cttaccggcg
     3361 acccgctcgg catggcgcgc cgaaccgttc gtgaaaatga acttgcgtcc atcgagccgc
     3421 gagatcgcct ctgcgagctc cggcatgtgc gacacggggg caagatcgat gtcatgcacg
     3481 aaatccatga agtcgtccgg cttcacatca tgcttcgcca tgagcccgga gagcgtggtg
     3541 ccgtgctcgt agtaaaggtc cttttgcacc ttccgcgccg ccacgggatc gagcccaagc
     3601 aaagtctgga tgaaggccga catccgctgg tcgatctgcg caaacagatt gcattcggcg
     3661 gggtaaagcg tgttgtcgag gtcgaaaatc cagagctgtg tcccggcaaa ggcttccgcg
     3721 acgctggccc tgccggccgc tggattagcc atccgcatca cgctcgatca gcgtgccgac
     3781 gccgtgctcg gtgaacagtt cgagaaggac cgcgtggtgt acgcgtccat cgagaatgac
     3841 aacgccctcc acaccctgct cgatcacgct caggcagcct tcgatcttgg ggatcatgcc
     3901 gcccgtgatc gtaccgtccg ccatcatcgc tttggcctca gaaacggtca ggcgatggat
     3961 aagcttcccg ttctggtcga gcacgcccgc aacgtcggtc agaagcagga ggcgcttcgc
     4021 cttgagcgcg cccgcaagtg cgctggcaaa tgtatcggcg tttatattgt aggtggtgcc
     4081 gtcgcg
//
LOCUS       DATMGX010000051         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_283317, whole genome shotgun sequence.
ACCESSION   DATMGX010000051 DATMGX010000000
VERSION     DATMGX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283317"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1311)
                     /locus_tag="VNR65_00850"
     CDS             complement(109..1311)
                     /locus_tag="VNR65_00850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ17251.1"
                     /translation="MNLTSSHPFWSVANGLPANYPSLQRNVSCDAVVIGGGITGALVA
                     VHLVEAGVKTLLLDKRDIGTGSTSASTALLQYEIDVPLRELIEKVGPVAATRSYQLCR
                     GAIGKLERLAARLKIDCSFKRKPSLFLARHQREIPELREEFKLRRKMGIELEFFDASA
                     VRARFPFSRSAALFSKDGGQVDPHRLTHGLLAAGRRAGLEVYDRTKMVNLEETRRGVR
                     VTTHNGCQIIARRAVIAAGFESKALLNIEAGTLKSTYALISEPLPAISGWYQQCLIWD
                     SGTPYLYLRTTTEGRVIVGGEDENFVNAKRRDALISQKTRTLAKKFGRLFPDLPLEVA
                     YAWAGTFGETKDGLAYIGVHPRFPHAYFALGYGGNGITFSLIAAEIIRDHFLGRTNRD
                     AHLFRFGR"
BASE COUNT          474 a          583 c          581 g          445 t
ORIGIN      
        1 aattcagccc gagcgcgctg ggagtatcgt cgcagcccga acgcgaccac ccgcagattt
       61 caaacagctt tgagcgcgct ccagtttcag aacgccaggc aagcaccatc agcgaccgaa
      121 acggaacagg tgggcatcgc gatttgtccg gccgaggaaa tggtcccgga tgatctcggc
      181 agcgatgagg ctgaaggtga tgccattccc accataaccc agcgcgaaat aggcgtgcgg
      241 aaatcgggga tgaacgccaa tgtaagctag gccgtctttc gtttcaccga aagtgccggc
      301 ccaggcatag gctacttcca atggtaaatc cggaaacagc cgcccaaact tttttgccag
      361 ggttcgggtc ttttgagaaa tcaacgcgtc ccggcgcttc gcgttcacga agttttcgtc
      421 ttcgccgccg acgatgacac gtccttccgt ggtggtccgc agatagaggt aaggcgtgcc
      481 gctgtcccaa atgaggcact gttggtacca tcctgaaatc gccgggagcg gttcactgat
      541 gagggcgtaa gtgctcttca aggttccggc ctcgatgttc aagagggctt ttgactcgaa
      601 cccggctgca atcacagccc ggcgggcgat gatctggcaa ccattgtgcg tggtgacgcg
      661 aactccacgg cgagtttcct ccaaattcac catcttcgtg cggtcgtaca cttcaagacc
      721 cgcacgcctc cccgcagcca gcaggccgtg agtcaggcgg tgcggatcta cttggccacc
      781 gtctttggag aacagcgcgg cggagcgtga gaagggaaag cgtgcccgaa ccgccgacgc
      841 gtcgaagaac tccaactcga ttcccatttt ccggcgcagt ttaaattcct ctcgaagttc
      901 gggtatctcc cgctgatggc gggccagaaa caagctgggc tttcgcttga agctgcaatc
      961 gatctttaag cgtgccgcca gtcgttccag tttgccaatc gcaccgcgac acaactggta
     1021 gctccgggtc gcagcgaccg ggccgacttt ttcgatcaac tcacgcaaag gaacatcaat
     1081 ttcgtattgc aacagggcgg tgctcgcgct ggtgcttccc gttccaatgt cccgcttatc
     1141 aaggagtaga gtcttcactc cggcttcaac cagatgaacc gccaccaatg ccccggtaat
     1201 cccaccgccg ataacgaccg cgtcacaaga aacattgcgt tgcaatgaag ggtagttcgc
     1261 cgggaggccg ttggcgaccg accaaaaagg atgactcgaa gtgagattca tgaggcttga
     1321 tagccgccta tgcgggtcac attgtagcca tgccagagca ggccggaatg gccttgggcg
     1381 aatcgggccg gttcaggtta gcaaaattgg cgctctttag gtgccaatgc ggacctatcg
     1441 cctgcgtcat tttttcaaac ggcgacctgg aagcttattt accgaacatc ttcgctatga
     1501 tgaagatgac gacggctccg aaaaggcctc cgcctccgag gatatatatc aaggagtaac
     1561 ccgcggcggc aaacattggt gatattaata gtgcaatcat attttgattt tcttctgtgt
     1621 tttgtttttc tttcgtggaa agttttctct tttttatatc ccgcacaata ggcggagaac
     1681 ctgacggggc gatatggggt gtttataacc ctgccgtcaa tgccaggagc cgctgcccga
     1741 aggctcatct ggccggcgaa atcgcgccgc ccggcgtctt aaaacgccat aagccacttc
     1801 agctagcggc gaagcaatag caccacaaca atgatgacga gaacgaggcc gaccgagccg
     1861 ccgccgatgt agagcgcact ggcaagaacc ggcgagaata atagatgcat ggtttgtctt
     1921 tcgtttttga tggcaatatg ttcaccatca cttttcgggg cacgctacgc gatgggccgg
     1981 tctttagcta tggggtgtaa ccctacctgg attagctcaa gcggacgcaa attccatgag
     2041 cgccgcgaag gcggagaagc cggcaccgaa cgcgaggagc atc
//
LOCUS       DATMGX010000052         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_282638, whole genome shotgun sequence.
ACCESSION   DATMGX010000052 DATMGX010000000
VERSION     DATMGX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282638"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1291)
                     /locus_tag="VNR65_00855"
     CDS             complement(14..1291)
                     /locus_tag="VNR65_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17252.1"
                     /translation="MYSVRIFTTADASLPPPNFGPNLTWDRLTEALRRELGPEAAGLL
                     AEPIPDAERAETHWHVEAASDPVPISALPAKDREIVLERLAELRARIRGYASRLEKAG
                     GDENARLASAFRSAILVPDDTAYVWSLDGNPILAGWGRRSEALERSSHVITRQTAAPR
                     PTAARAGVFPGQAVEKDIARQSGRRAGTAWLLPFLLWLLFIGTALFTGYLLLPACAVD
                     LPLVRTFFDTCRGPAVDRLVALREQNSSLRRAIESAETKVALSRPPCPADNAIKKTER
                     PTPHDKETTATQPTGEETKSRAEEAGGKHGKLDITLSWNGHADLDLHVQCPGGRLSYK
                     DQERHSCEGGEFEIDRNAGAELVDNPVEHVSWAGDPPAGDYRVDVQLYDYHDAPEGPV
                     PFTVVITEGGASRTYSGTVGTKGASVKVTEFRR"
     gene            complement(1297..>2097)
                     /locus_tag="VNR65_00860"
     CDS             complement(1297..>2097)
                     /locus_tag="VNR65_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HWJ17253.1"
                     /translation="LPAGPDAPHESASNIGQLLDSATVLIVVVKYPSYSQGSGFFVSD
                     RHIVTNHHVIRDAQAGDIFVIGRGIGGVRRAGLLAKTSPAPTERDINPDVAVLEIEPL
                     AGQQGLKLGVTPSKLSTAYVAGFPGFLTEKDHDFTNFMTQLASAAATRNLDSLLSKAK
                     FTPPSADLRYGRINNSIRSGSRDLPILIHDMQLAQGNSGGPLVDACGRLGGINTALFA
                     NEEGAQQGNVAQDVSVVRDFLKENNIPFRSEESACNPAVASNEPPERR"
BASE COUNT          401 a          631 c          634 g          431 t
ORIGIN      
        1 ttcttgcagc gaatcaccgt ctgaactccg tcaccttaac gctcgcgccc ttggttccca
       61 ccgtgccgga gtatgtgcga gaggcgccac cctcggtgat cacaaccgtg aacggaaccg
      121 ggccctcggg cgcatcatgg tagtcgtaaa gctgaacgtc cacacggtaa tcgcccgctg
      181 gaggatctcc tgcccagctg acatgttcga ctggattatc gacgagctca gccccggcat
      241 tccggtcaat ctcgaactct ccgccctcgc atgaatgccg ctcttggtcc ttgtatgaca
      301 gccgtcctcc ggggcattgc acgtgaagat caaggtccgc atgcccattc cacgagagcg
      361 tgatgtcgag cttgccatgc ttgccgccag cctcctcggc gcggctcttg gtctcttccc
      421 cggttggctg agtagctgtt gtttccttat cgtgcggagt aggcctctca gtctttttta
      481 tggcattgtc cgcagggcat ggcggccggc taagcgcgac cttggtttcc gcactctcaa
      541 tggcccttct aagcgagcta ttttgctcgc gaagagcaac gagccggtcc actgcgggac
      601 cacggcaagt atcgaaaaac gttcggacga gcggaagatc gacagcgcag gcgggcagaa
      661 gcaagtagcc ggtgaagaga gccgttccga tgaataaaag ccacagcaaa aaaggcagga
      721 gccacgccgt tccggcacgc ctgccggatt gccgggcgat gtctttctcc acggcttgcc
      781 ctggaaaaac gccagcgcgc gcggctgtgg gacgaggcgc cgccgtctga cgcgttatga
      841 catgggagga tcgctccaat gcttcgcttc ggcggcccca gcctgcgaga ataggattgc
      901 cgtccaggga ccaaacgtag gccgtatcgt ccggcacgag gatagcgctc cgaaaagcgg
      961 aagcaagccg ggcattctcg tcgccgcctg ctttctcgag tcgggacgcg tagccgcgaa
     1021 tgcgcgcccg cagctcggca agtctctcca gcacgatttc gcggtctttc gcaggcaagg
     1081 ctgaaatcgg gaccggatcg cttgccgcct cgacatgcca atgggtctcc gcacgctcag
     1141 cgtctggaat cggctccgct aataaaccgg ccgcctctgg accaagctcc cggcgcaagg
     1201 cttcggtgag gcgatcccat gtcaggttcg ggccgaagtt gggcggcggc aatgaggcat
     1261 cggccgtggt gaagatgcga acgctgtaca tgcctcctat ctccgctctg gaggctcgtt
     1321 ggaagcgacg gcagggttgc aggcgctctc ttcgcttcgg aatgggatgt tgttttcttt
     1381 cagaaagtcg cggacgaccg acacatcctg ggccacgtta ccctgctgag cgccctcttc
     1441 gttcgcgaac aaggcggtgt ttattcctcc caggcgcccg caagcgtcga caagcggccc
     1501 gccgctgttt ccctgggcca gctgcatatc atggataagg attggaaggt cccgcgaacc
     1561 cgacctgatt gagttgttga tgcggccgta acgcaaatca gcgctgggcg gcgtaaattt
     1621 tgccttggag agcagactgt ccaggttccg cgtagcggct gcagaggcga gctgcgtcat
     1681 gaagttggtg aagtcgtggt ctttctcggt caggaagcct gggaaccccg cgacataggc
     1741 ggtcgacagt ttcgatggcg taacgccgag cttcagaccc tgctggccag ccaaaggctc
     1801 aatttccaag accgccacgt ccgggtttat gtctcgctcc gttggcgcgg gcgatgtctt
     1861 tgcaagcagt ccggcgcgcc gcacgcctcc tatgccgcgg ccgatgacaa aaatatctcc
     1921 tgcctgggca tcccttatca catggtggtt cgtgacaata tgacggtcac tgacaaaaaa
     1981 tccgcttccc tgcgagtatg atggatactt gacaacgaca atgagaacgg tggcgctatc
     2041 gagcaattgg ccaatattgg aggcgctttc gtggggtgcg tccgggccag ccggaag
//
LOCUS       DATMGX010000053         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_19876, whole genome shotgun sequence.
ACCESSION   DATMGX010000053 DATMGX010000000
VERSION     DATMGX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19876"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="VNR65_00865"
     CDS             <1..1508
                     /locus_tag="VNR65_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HWJ17254.1"
                     /translation="ANAVALEPAYYSASLDDIDAAARLAADAFPHFAKLSGRERAEFL
                     RRIAEGLTAASGAIVGRANAETGLPVARLQGELARTTGQLKLFAELVEEGSWVDARID
                     EADRERKPLPRPDVRSMLRPIGPVAIFGASNFPLAFSVAGGDTASALAAGNPVIVKAH
                     PAHPGTSEIVGRIVQEAVRVSELPGGVFALLFDAGIEVGTALVEHPEVRAVAFTGSAT
                     AGQALMRIASQRPEPIPCYSEMGSTNPLFILPGAMRERGPVLAQALQASFTLGSGQFC
                     TKPGLVFVPKKEDRKFMEALRSGVGSLEQQGMLTAGIATKYKDAVLQRLHENRAQSIA
                     GTAPGALASNASCFPMLFEVSLPEFLKEESLEEEIFGPTTLLVRYGSEDELLVAAGQL
                     HGHLTATIHGTEEDLSNSSELVRVLEGKVGRILFNGFPTGVEVCHAMVHGGPFPATAD
                     GRSTSVGTRAILRFARPVCYQDFPDASLPPELQQTNPLRIMRMVNGDLGRD"
     gene            complement(1540..>2162)
                     /locus_tag="VNR65_00870"
     CDS             complement(1540..>2162)
                     /locus_tag="VNR65_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWJ17255.1"
                     /translation="GVTYFRSRSARIEESKDAGGGTFYDRVYNAERPELFFKATARRV
                     IGHRGAVRIRSDAKWSVPEPELTLLVNPAGEIIGYTIGNDMSSRDIEGENPLYLPQAK
                     VYDGSCAIGPCVLLSGEPLSKSTTIHIEIHRSNKIAFNGSTTLAELKRDPKQLVEYLF
                     RDNSFPCGAYLMTGTGIVPGDGFTLASGDLIKISIDGIGMLENTVA"
BASE COUNT          433 a          581 c          688 g          460 t
ORIGIN      
        1 cagcgaacgc ggttgcgttg gaaccagcct actattcagc atctctggac gatattgatg
       61 ccgcggcaag actggcggcg gacgcctttc cgcactttgc taagctgagc ggccgcgaga
      121 gggcggagtt tctgcgaagg atcgccgagg gattgactgc agcgagtgga gctattgttg
      181 ggcgcgcaaa tgctgagacc ggcttgcccg tggcgcgatt gcagggtgaa ctggctcgta
      241 caaccggaca actcaaactc ttcgctgagt tggtcgagga ggggtcctgg gtggatgccc
      301 gtatcgatga ggccgaccgc gagcgcaaac cgcttcctcg tccggacgtg cgctcaatgc
      361 tgcggccgat cggcccagtg gcgatctttg gcgcgagtaa ttttccgcta gccttttcgg
      421 tcgcgggggg agatacagcc tctgcgttgg cagcgggcaa tcccgtgatc gtgaaggcac
      481 atccggcaca tcctggaacc agcgagattg tgggaaggat cgtccaggag gctgtgcgcg
      541 tgagtgaact gcccggcgga gtgtttgcgc tgctgttcga cgccggcatc gaagtgggca
      601 ctgctttggt ggagcatccg gaggtgaggg ctgttgcatt cactggctcg gccacagcgg
      661 gccaggctct catgcgaatt gcttcgcaga ggccggagcc aattccctgc tactccgaga
      721 tgggcagtac caacccgctg ttcatcctgc caggagccat gcgagagcgc ggaccagtgc
      781 tggcccaggc gttgcaggcc tcgttcacac tggggtcggg acaattctgc acgaagccgg
      841 gattggtgtt tgtaccgaag aaggaagatc ggaaattcat ggaggcgctg cggagcggag
      901 tgggctcgct ggagcaacag ggcatgctga cggctggcat tgctacaaaa tataaggacg
      961 ctgttctaca gcggttgcat gagaacaggg cacaaagcat cgctggcact gcaccgggcg
     1021 ccctggcgag caacgccagc tgttttccaa tgcttttcga agtatcactt ccggagttcc
     1081 tgaaggagga atcccttgag gaagagatct ttggcccgac gacactgctg gtgcgctacg
     1141 gtagtgagga tgaacttctg gtcgcagcag ggcagttgca tggacacctg acggcgacta
     1201 ttcacgggac agaagaggac ctttccaatt cgagcgaact agtgcgtgtg ttggagggca
     1261 aggttggccg aatacttttc aacggatttc ccacaggagt ggaggtttgc cacgctatgg
     1321 tgcacggcgg gccatttcca gctacggcgg acggccgatc aacctcagtg ggcacgcggg
     1381 ccattctccg gtttgcgcga cctgtttgct accaggattt tcctgatgca tccctgccgc
     1441 cagaattgca acagacgaac cccctgcgaa ttatgcgaat ggtaaacggc gacctgggcc
     1501 gcgactagca attgaccagc ggtagcgcga cgtcttcgac taagcgaccg tattctccag
     1561 catcccgatg ccatcgatgc tgattttgat caggtctccg ctcgcgaggg tgaagccatc
     1621 gccgggcaca atacctgttc ctgtcatcaa atatgcgccg caaggaaagc tgttgtcgcg
     1681 aaacaggtac tcgacgagtt gcttgggatc gcgcttcaat tcggccagag ttgtgctgcc
     1741 gttgaaggcg atcttatttg accgatggat ctcaatatgg atggtcgtgc tctttgagag
     1801 gggctctcct gagagcaaaa cgcacggacc tattgcgcag ctaccgtcat aaacctttgc
     1861 ctgcggaagg tagagaggat tttcgccttc aatatcgcgt gagctcatat cgttgccgat
     1921 cgtgtatccg atgatttcgc cggcgggatt gaccagcaaa gtcagctcgg gctcgggcac
     1981 agaccacttt gcatccgatc ggattcgcac ggcaccgcga tgaccgatga cgcgccgggc
     2041 ggttgctttg aagaatagct cggggcgttc tgcgttgtag acgcggtcgt agaaggtccc
     2101 gcccccggcg tctttcgact cttcgatgcg ggcactacgg ctgcgaaaat aggtcacgcc
     2161 tg
//
LOCUS       DATMGX010000054         2299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_19857, whole genome shotgun sequence.
ACCESSION   DATMGX010000054 DATMGX010000000
VERSION     DATMGX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19857"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VNR65_00875"
     CDS             complement(<1..513)
                     /locus_tag="VNR65_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWJ17256.1"
                     /translation="MDGLSPLLERFTLNARVFFAGTLCSVSSFEGQPGIGTIHVLRGG
                     CLRVEQHGGARLEISEPSVLFYPRPYAHVFQVDLEAGAELVCAWIDFGAGLGNPVLRG
                     LPEMILAPLARIPGIDTALISLFDEAFAKRPGRQAALNRLVEYILVLLLRYAMEMKSV
                     SGSVLAGLADP"
     gene            624..1175
                     /locus_tag="VNR65_00880"
     CDS             624..1175
                     /locus_tag="VNR65_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HWJ17257.1"
                     /translation="MQRINALTPSTAPAAAKTLLDAAHKKIGMLPNLYATLAHSPAAL
                     QAYLQQTEALTAGVLAPALREQLALVTAGVNGCDYCASAHTLLGKRAGLPAGEAASNL
                     KGQSADTKTQAALDFASAILSSRGHVSDAQLQHVRAAGFNDAQIVEIVAHLAMNTFTN
                     YLNNIAKTVIDFPHVSTATAHAA"
     gene            1401..1898
                     /locus_tag="VNR65_00885"
     CDS             1401..1898
                     /locus_tag="VNR65_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HWJ17258.1"
                     /translation="MQFDPQARILIAGQAPGSKVHASGVPFQDASGDRLRDWMGIDEA
                     SFYDATQVAILPMGFCYPGTGKSGDTPPRPECAETWRAGLMARLVNVRLTLVIGQYAQ
                     AWHLGEARKPTLTATVQAWRDYWPASLPLPHPSPRNNIWLKRNAWFETDVLPRLRCRV
                     AELKR"
BASE COUNT          512 a          739 c          648 g          400 t
ORIGIN      
        1 cgggtctgca agccctgcga gcacgcttcc gctgacactc ttcatctcca tcgcgtagcg
       61 cagcagcagc acaagaatgt actcgaccag ccgattgagc gccgcctgtc gtcccggccg
      121 tttggcgaag gcttcatcaa agagcgaaat tagcgcagtg tcgatgccag gaatgcgggc
      181 cagcggcgcc agaatcattt cgggaagccc acgcaacaca gggtttccca ggccggcacc
      241 aaaatcgatc cacgcgcaga cgagctccgc gcccgcctcc aggtcgactt gaaacacatg
      301 tgcatagggg cgcgggtaaa acagcaccga cggctcggaa atttccaagc gagcgccacc
      361 atgctgctcg acccgcaggc acccgccccg caatacgtga atcgtaccga tgccaggctg
      421 tccttcgaag gaagacacgc tgcacagcgt accggcaaag aagactcgcg cgttaagcgt
      481 gaatcgctca agcaggggtg aaaggccgtc cacgagacga tcagtatgtc atttgatact
      541 cctgagcacc attggtagct caatcaggcg cacagtctcc ctcgacgagc ccattcacca
      601 cctactcatt ggcggagact gccatgcaaa gaattaatgc tctaaccccg tccaccgccc
      661 ctgcggccgc caaaaccctg ctcgatgccg cacacaagaa gatcggcatg ttgccgaatc
      721 tgtacgcgac gctcgctcac tccccggcgg cgcttcaggc ctatctgcag cagactgaag
      781 cactcaccgc gggggttctt gccccggccc ttcgcgaaca gcttgcgctc gtcaccgccg
      841 gcgtcaacgg ctgcgactat tgcgcctctg cgcacacgct gctgggcaag cgcgccggac
      901 tcccggcagg cgaagcggcc agtaacctca agggtcagtc agccgacacc aagactcagg
      961 cggcgctgga tttcgccagc gcgattctca gcagccgcgg ccatgtctcc gacgcgcagc
     1021 tgcagcacgt gcgtgccgcc ggattcaatg atgcgcagat cgtcgaaatc gttgcgcacc
     1081 tggcgatgaa cacgttcacc aattatttaa acaacatcgc gaagacggtc atcgacttcc
     1141 cgcacgtgag caccgctacc gcccatgcgg cctgaccaga atccgcttcc ggaaaacaac
     1201 atcatgaaaa cgtcaactcc ggttctctgc ctggcggcag acatggaact gggcacgact
     1261 aacgcctacc tcggcgtgat tgcctacttg aagaagatat cgcccgcgcc atgagaatca
     1321 ccgcgacccc gcggctgttg aaagaaatcc gggcgtgcac cttgtgtacg gcgcacctgc
     1381 ccttgggacc gcgaccaata ttgcagtttg accctcaggc caggatactg attgccggcc
     1441 aggcccccgg aagcaaagta cacgctagcg gtgttccttt ccaggacgcc agcggcgatc
     1501 ggctccgcga ttggatgggt atcgatgagg cgagtttcta tgacgcgaca caagtggcca
     1561 tccttcccat gggtttctgc tatccgggca ccggaaagtc cggtgacacc ccaccccgac
     1621 ccgaatgcgc cgagacctgg cgcgcggggc tgatggcaag actcgtcaac gtgcggttga
     1681 cgctggtcat cgggcaatac gcacaagcgt ggcatctcgg cgaggcccgg aaacccacat
     1741 taaccgcgac tgtgcaggca tggcgtgatt actggccagc gagtctcccc ctaccgcacc
     1801 cgagcccacg caacaatatc tggctgaaga gaaatgcctg gttcgaaacc gatgtcttgc
     1861 cgcgcctgcg ttgtcgagtg gccgaactga agcgttaggt cgggtaatcg cggcgctcaa
     1921 gacacgaagg cacaaagccg aatacttcag tcaagcagct caagagacca tccaaatgcc
     1981 gggatccgcg cgcagcggaa gagaaccagc ctaacatgag gcggagtcgc ggctgggcag
     2041 aaagccggcg atatgactca aggatcaccc aaggcgggcg attcctattt gcgagcaagc
     2101 ttttacttgt agactttccg cccagagaat ggaacgtaaa ggccgcatcc gagatcgttc
     2161 cattcttttt cgtacctgat tgtcgcaccc agctgaccgg aatgtccggg ccagaggcac
     2221 aagcgttacc ctttctcggc ctagatgccg cggccgcgtt gggtctggcg gaatcggccg
     2281 aagccgcgtg gctgaaggt
//
LOCUS       DATMGX010000055         4305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_282106, whole genome shotgun sequence.
ACCESSION   DATMGX010000055 DATMGX010000000
VERSION     DATMGX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282106"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VNR65_00890"
     CDS             complement(<1..969)
                     /locus_tag="VNR65_00890"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HWJ17259.1"
                     /translation="MDRRDVLKLSAGFAAALFLAREAASASAAESLTSLPMGEAAPFS
                     EDIVIERARQLSQAPYAPPQDTMPAAYKDLNYDQYRAIRFKKDLALWRHDNVSFTAEF
                     FSAGYIYLAPVQIFVADGGKAAELKYLPDLFTYGPEVSRPPENTNPGFSGFRLHTPIN
                     RPDVMDEFAVFQGASYFRSKAPGQDYGLSARGLAINTAQPPADEFPIFRSFWLVKPAP
                     GASVVTVFALLDSVSVSGAYKFVISAGQNTVMDVDCTLFPRKTMNYAGITPLTSMFYF
                     SPGDDYRADDVRQRVHDSDGLSIWNGKGEHIWRPLINPSRNQFSSFA"
     gene            complement(1076..2617)
                     /locus_tag="VNR65_00895"
     CDS             complement(1076..2617)
                     /locus_tag="VNR65_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HWJ17260.1"
                     /translation="MDRRTWLKLAASTAALATFPLRVDAQINLSEASADSQPFSEELL
                     DAFARRRADEPYKAPEKRVDEALAGITYEQYNRAIVYREDQAIWRKDDGPFWLEPYHT
                     AGPLFAFPVELFTVDAGRAIKIPYSAEAFEFNPPAKQPATTAQSDFAGFRALGQIDRT
                     GVFRDFLSFLGASNFRSFATGQVFGVTARALAINTGQTGGEDFPNFRSFWIERPKPID
                     QSLVIHALLDSVSAVGRYKFTVTPGFDTIIETEAVIYPRRRIPYAGIAPIASRFFFGP
                     GVPAKRKDYRPRVHDSEALCIVNGAGEQIWRPLINPERLQFSVFVDKGPKGFGLIQKE
                     RAFASYQDLDQQFDKRPSLWIEPLGDWGEGSIDLIELPAPEEFNENIVCFWRPKDGLG
                     PGIGHRYRYRMHWCWNPPIESKKATVQQTRVGETKAGEVAFVVDFANTELCEGCNAAA
                     LMASVTASAGEVRNPRLAAGPSAANQRLRFEYAPSGSDPVDLRAQLVADGKPISETWI
                     FRWAR"
     gene            2975..>4305
                     /locus_tag="VNR65_00900"
     CDS             2975..>4305
                     /locus_tag="VNR65_00900"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HWJ17261.1"
                     /translation="MGHWQLASVLAFVLAAVGWGSAMAAESGPRHHVEVSLLAEKSAA
                     EPGKTLTVAVQERIEPGWHTYWINPGDSGEPTSIEWSLPQGFSAGPILWPIPHVISVG
                     PLAEYGYDDEVVLLTEIQLPEKIEGDSITLSAKVSYLVCREICIPEEKHVELTLPVKS
                     DGAASPSAFAPQIEKARSALPLPLPGSASYSANRAKELLRLTVSTDEKLLKGVKAARF
                     YPLTWGSLSNPAPQPMKISGGQLTLDLKQGDTKETPAKLEGLLVLTNGGGEGGRTGYT
                     VSASRGPDSAAAGSVLDPGGGDGVGSSAVQSSAAARGVSGPGIAVLLAFLGGAILNLM
                     PCVFPVLALKALAFAKKAEHGHWQQGIAYLGGVLVSFGAIAAFIVAFRNSAEALGWGF
                     QFQSPAFVLVLAILFFVMGLSLSGVVSFGAGMMSIGDSLSRRPGNAGYFFTG"
BASE COUNT          839 a         1218 c         1323 g          925 t
ORIGIN      
        1 agcaaaagaa gaaaattggt ttcgactcgg atttatcagt gggcgccaga tgtgctcgcc
       61 tttgccgttc cagatggaaa gaccatcgga gtcgtgcacg cgttgccgta cgtcgtccgc
      121 gcgatagtcg tctcctgggg agaagtagaa catgctcgtc aagggggtaa tcccggcata
      181 gttcatcgtt tttcgcggaa aaagcgtgca gtccacatcc atgacagtat tttgccctgc
      241 ggagatgaca aatttatagg cgcctgagac gcttacactg tccagcaatg caaaaaccgt
      301 gacaacggaa gcacccggcg ctggcttgac cagccagaag ctccgaaaga ttggaaactc
      361 atccgccggc ggttgcgccg tgttgatcgc gagcccgcgc gcagacagac catagtcctg
      421 ccccggcgct ttcgaccgga agtaacttgc tccctggaaa accgcaaact cgtccatgac
      481 gtcaggcctg ttgatgggcg tgtgcaagcg aaaccctgaa aagcctggat ttgtgttttc
      541 cggcggccgg ctcacttcgg gaccgtatgt gaacagatca gggaggtatt tcagttccgc
      601 agccttgcct ccatctgcga cgaaaatttg gaccggggct aagtagatat atcccgcgct
      661 gaagaattcg gcggtgaagc ttacattgtc atgcctccac agcgccaaat ccttcttgaa
      721 gcggattgcg cgatactggt catagttgag gtctttgtat gcggctggca tggtgtcttg
      781 cgggggagcg tagggcgctt gcgaaagctg gcgtgcccgt tcgatcacga tatcctcgga
      841 gaaaggcgca gcctcgccca tgggcaggct ggtgagagat tcggcggcac tggcggacgc
      901 cgcttcccgc gccagaaaca gagccgcggc gaagcccgcg ctgagtttga ggacgtcccg
      961 ccgatccatg gtgaatgaga gcccttagag gttttctaga atgacgcgtt gtggatgagc
     1021 tttaggttgg gctagagaac ggctcgacga gcctcgctca gcgaactgcg acgaactacc
     1081 gcgcccagcg aaagatccat gtttccgaaa tcggctttcc gtcggcaaca agttgcgcgc
     1141 gcaaatcaac cggatcgcta cctgacggcg cgtattcgaa ccggagccgc tggtttgctg
     1201 cgcttggccc ggcggccagc cttggattgc gcacttcacc cgccgaggct gtcacactcg
     1261 ccatgagcgc ggcagcgttg cagccctcgc aaagttccgt gtttgcaaaa tcgaccacga
     1321 aagcaacctc tccggctttc gtctctccga cgcgtgtttg ctgcaccgtc gccttcttgc
     1381 tttcgatggg agggttccag caccagtgca tgcgataacg gtagcggtgg ccgatgcccg
     1441 ggccgagccc atctttcgga cgccagaagc agacgatatt ctcgttgaac tcttccggcg
     1501 cgggcagttc gatgaggtcg atggagcctt cgccccagtc tccgagcggc tcgatccaga
     1561 gagacggtcg cttgtcgaat tgctggtcaa ggtcctgata gctggcgaat gcccgctctt
     1621 tctggatgag gccaaaccct tttggccctt tgtcgacgaa gacggaaaat tgcagccgtt
     1681 cgggattgat aagcggccgc caaatctgtt cgcctgcgcc attgacgatg cagagcgctt
     1741 cggaatcgtg gacgcgtggc cggtaatcct tccgcttggc cggcacgccc ggcccaaaga
     1801 agaagcggct cgcgataggc gcaatgccgg cataaggaat gcggcggcgc ggatagatca
     1861 cagcctcggt ttcgatgatg gtgtcgaaac cgggcgtcac agtgaatttg taacgcccga
     1921 ccgcgctcac gctgtcgaga agtgcgtgaa tgacgaggga ctggtcgatc ggttttggcc
     1981 tctcgatcca gaagcttcgg aagttcggga aatcctcgcc gcctgtctgg cccgtattga
     2041 tggcgagtgc tctggcggtc acgccgaaaa cctggcccgt cgcgaaagac cgaaagtttg
     2101 aggccccaag gaagctcaag aagtccctga aaacgccggt cctgtcgatt tggccaagcg
     2161 cgcgaaatcc cgcgaaatca gattgggcgg ttgtggccgg ctgcttggcg ggtggattga
     2221 actcgaaagc ctccgcggag tacggaattt tgatcgctct gcccgcgtcg accgtgaaaa
     2281 gctcgaccgg aaacgcaaag agtgggcccg ccgtgtgata gggctccagc cagaacggtc
     2341 catcgtcctt gcgccagata gcctgatctt ccctataaac gatggcgcgg ttgtattgct
     2401 cataggtgat gccagccagc gcttcatcga cgcgcttttc cggcgccttg taaggctcat
     2461 cggcacggcg ccgcgcaaag gcatcaagca gctcttccga aaaaggctgc gaatcggcgc
     2521 ttgcctcaga gaggttgatt tgcgcatcga cccgaagcgg aaaagtcgcc agggcggctg
     2581 tcgatgccgc gagtttcagc catgtgcggc ggtccatccc ccactcccga aaactgtgcc
     2641 gttgcaccgc gatcgcaaaa ggcaaaatgt ctccagctgg cggatgcaca aggccggcca
     2701 aagtctggat acaaaaaatt atttcgcccc gctttagacg ttgacgcttt ggagtttttt
     2761 ggggcggttt tgaggcgctt ttcgcgtttg caaaaaaacg ccgcagacgt cccgctttgg
     2821 gtttcataat tgcgcgttgc aaaagaagag aatgcctgaa attcctgcgg ttttgagacc
     2881 ttcgggcgcc ggcaccatga tttctggccc ttgatcttcc ttggcagaaa ggcctatttc
     2941 agcaaacgtg aagggcattg gagagcaggt tcgaatggga cattggcagc ttgcgtctgt
     3001 cttagcgttt gttctggccg ccgtggggtg gggaagcgct atggccgctg agtcggggcc
     3061 gcgtcatcac gtggaggtca gcctgctcgc ggagaagtcc gctgccgagc ccggcaagac
     3121 gctaacggtc gcggtgcagg aaaggatcga gccgggctgg cacacttact ggatcaatcc
     3181 aggcgattcg ggcgagccga cttccattga gtggagcttg ccgcaaggtt tctcggcggg
     3241 accgattctt tggccgattc ctcatgtcat ctcggtgggg ccgctcgccg agtatggcta
     3301 tgacgatgag gtcgtgctcc tgacggaaat ccagctcccc gagaagattg aaggcgatag
     3361 cataacgctg agcgcgaaag tctcttatct cgtgtgccgg gagatttgca ttccggaaga
     3421 gaagcatgtc gagcttacgc tccccgtgaa gagcgacggg gcggcatccc catccgcttt
     3481 tgccccacag atcgaaaaag cgcgctcagc tctacccttg ccgttgcccg gctcggcttc
     3541 gtactcggcg aaccgggcca aagagttgct gcggctgacg gtttcgacag acgagaagct
     3601 tctcaagggc gtcaaagccg ctcgcttcta ccctttgacg tggggctctc tttccaaccc
     3661 cgcgcctcag cccatgaaaa tttccggcgg ccaactcacg ctcgacttga agcagggtga
     3721 tacgaaagag actccggcaa agctcgaagg cttgctcgtt ctgaccaatg gcggcggcga
     3781 gggcgggcga accggctaca ctgtttccgc ttcgcgcggc ccggactcgg ctgctgctgg
     3841 cagcgtcctc gatcctggcg gcggagacgg cgtggggagc agtgctgtcc aatcatccgc
     3901 ggcggcgaga ggtgtttcgg ggccgggcat cgccgtgctg ctggcttttc tcggcggcgc
     3961 tattctcaac ctgatgccat gcgtcttccc ggttctcgcg ttgaaggcgc tcgcctttgc
     4021 aaagaaggcc gagcacggtc attggcagca gggcatcgct tatctcggcg gtgtgcttgt
     4081 gagcttcgga gccattgccg cgttcatcgt tgctttccgc aacagcgcgg aggctttggg
     4141 ctggggcttt caattccagt cgcccgcttt tgtattggtg ctggccatcc tgttcttcgt
     4201 gatgggcctt agcctgtccg gcgttgtgtc attcggggcc gggatgatgt cgattggcga
     4261 cagcctttcc cgcaggcccg gaaatgccgg ttacttcttt accgg
//
LOCUS       DATMGX010000056         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_19389, whole genome shotgun sequence.
ACCESSION   DATMGX010000056 DATMGX010000000
VERSION     DATMGX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19389"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="VNR65_00905"
     CDS             <1..1303
                     /locus_tag="VNR65_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HWJ17262.1"
                     /translation="MTSHAAVVARGMGRPCVSGASALRINTELGTLTAGDVTLKEGEV
                     ITIDGSTGQVIRGQVAMSQPELTGDFATLMEWADSVRRMKVRTNADTPNDAAQARKFG
                     AEGIGLCRTEHMFFDATRIMAMREMIVASDENGRRTALAKILPMQRQDFVELFQIMGG
                     LPVTIRLLDPPLHEFLPHGDEEIKELAASLKMDASALRARLSYLKEQNPMLGHRGCRL
                     AVSYPEIAEMQARAIFEAAVEAGKKTGSPVVPEVMVPLIATKAELDIVKAIIDRVAKE
                     VETASGTSISYHVGTMIELPRAALRAGDIAKSAEFFSFGTNDLTQTTYGLSRDDSGTF
                     LGDYLAQGIFDIDPFVSLDPEGVGELVQIAADRGRKTRPGIKLGICGEHGGDPASIEI
                     CEKVGLDYVSCSPFRVPVARLAAAQAAVRSKGKGHGTHSTA"
     gene            1409..>2305
                     /locus_tag="VNR65_00910"
     CDS             1409..>2305
                     /locus_tag="VNR65_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWJ17263.1"
                     /translation="MNAPRDLNLACLSSKAQIAAGLSAIEASPGSMSALLDEAYENPK
                     GIVIGITGPPGAGKSTLINALISEWRAQGKTVAVIAVDPSSRATGGALLGDRVRMRTD
                     PTDGGVFIRSLAARGRLGGLADIAYPSVALLRALYDKIIVESVGVGQSETDIATVSDT
                     VVLCIQPASGDSLQFMKAGIAEIPDIAVVTKADMGAAATRALADLKGALSLSHREKGA
                     WEPECISVSSTSGDGSLRQLFAAFARHEHWLLSENRLASGREKKAQAWVRAEIQADFG
                     RRGLDAAAQGLFQSWNNRPFSTS"
BASE COUNT          456 a          698 c          714 g          437 t
ORIGIN      
        1 catgacaagc cacgcagccg ttgtggctcg cggcatggga cgcccctgcg tctccggcgc
       61 gagcgctctt cgcatcaata ccgaattggg cacgctcacc gcaggcgatg tgacgctcaa
      121 ggaaggtgag gtgatcacga tcgatggctc taccgggcag gtgatccggg gtcaagttgc
      181 catgagccag ccggaactca ccggcgactt cgcgaccctt atggaatggg ccgatagtgt
      241 gcggcgcatg aaggtgcgca cgaacgccga cacgccgaac gatgcggctc aggcgaggaa
      301 gtttggagcg gaaggtatcg gcctttgccg taccgagcac atgttcttcg acgcaacccg
      361 catcatggcg atgcgcgaga tgatcgtggc ttccgacgaa aacggcaggc gcaccgcgtt
      421 ggcgaagatt ctgccgatgc agcgccagga cttcgtcgag ctattccaga tcatgggggg
      481 gctgcctgtc acgatccgcc tgctcgatcc gccgcttcat gagttcctgc cgcatggcga
      541 cgaggagatc aaggaactgg cggcgtcgtt gaaaatggat gcttccgccc tacgcgcccg
      601 gctcagctac ctcaaagagc agaacccgat gctcgggcat cgcggttgcc gtcttgccgt
      661 ctcctacccg gagattgccg agatgcaggc acgggcgatt ttcgaggcgg cggttgaagc
      721 gggcaagaag acagggtcgc cagttgtgcc cgaagtcatg gttcctctga tcgccaccaa
      781 agcggaactc gacatcgtca aggcgattat cgacagggta gcgaaggagg ttgaaactgc
      841 ctccggaacc tcgatttcct atcatgtcgg aacaatgatc gagctgccgc gtgcggcgct
      901 ccgggccgga gatatcgcga agtcggccga gttcttctcc tttggcacaa acgaccttac
      961 gcagacgacg tatggtctga gccgcgacga ctcgggcaca ttccttggcg attatctagc
     1021 ccagggcatt ttcgacatcg atccgttcgt ttcactcgat cccgaaggtg tgggcgaact
     1081 cgtccagatc gcagcggacc gcgggcggaa aacgcggccc ggcatcaagc ttggcatctg
     1141 cggcgagcat ggcggtgatc cggcgtccat cgaaatttgc gagaaggtcg gccttgacta
     1201 tgtctcctgc tcgccattcc gggtgccagt cgcgaggctt gcggcagctc aggccgccgt
     1261 ccgctcgaag ggtaaggggc acgggacaca ctccaccgcg taaaggctgt ggcgggcagg
     1321 tcgcccgcct cgctgtccgt tttgtaaaca gctcgccacc gggagcgccc tgatcgctcc
     1381 cggtggcgag gaaacaaggc ataaggcggt gaacgcgcct cgagacctga accttgcctg
     1441 cctttccagt aaagctcaga tcgccgcggg cctttccgcc atagaggcga gccccggctc
     1501 gatgtcggcc ctgctcgacg aggcctatga gaatcccaaa ggcatcgtca tcgggataac
     1561 cgggccgccc ggcgccggca aatccacact catcaacgcc ctgatctcgg aatggcgcgc
     1621 tcaagggaaa accgtcgccg ttattgccgt cgatccatcg tcgagagcaa ccggcggtgc
     1681 actcctaggc gaccgcgtcc gcatgaggac cgatccaacc gacggcggcg ttttcattcg
     1741 ctcgcttgcc gcgagaggcc gactcggcgg cctcgccgac atcgcttatc catcggtggc
     1801 actgcttcgc gcgctttatg acaagatcat cgtcgaaagc gttggcgtcg gtcagtcgga
     1861 aacggatata gctacggtgt ccgataccgt tgttctttgc atccagcctg cttctggaga
     1921 ctcgctccag ttcatgaagg ccggcatcgc cgaaattcct gacatcgccg tcgtcaccaa
     1981 agctgacatg ggcgcagctg caacgcgcgc gctggctgat ttgaagggcg ctctgagcct
     2041 gtcccatcgg gaaaaaggag catgggagcc ggagtgcata agtgtttcgt caacgagtgg
     2101 cgacggcagt cttcgccagc ttttcgccgc gttcgctcgc cacgagcact ggcttctgag
     2161 tgaaaaccgc ttggcttcag gccgcgagaa aaaagcgcag gcatgggtgc gagccgagat
     2221 acaggcagac ttcggccgaa gaggcttaga tgctgctgcg caagggcttt ttcaatcctg
     2281 gaataacaga cccttttcca cgtcc
//
LOCUS       DATMGX010000057         3587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_468648, whole genome shotgun sequence.
ACCESSION   DATMGX010000057 DATMGX010000000
VERSION     DATMGX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468648"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VNR65_00915"
     CDS             <1..564
                     /locus_tag="VNR65_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWJ17264.1"
                     /translation="HSERVAEYSVAIAQELGMDKEEIERVHLAALVHDIGMISVGDAS
                     LKKNRLLAVREFEILRAHPARGAAILRSIESLHDLIPAVELHHEALDGTGYPYGLNDK
                     DIPLMARILAVADTFDSMICADPFQPGLTPADAVDALEDLAGTRLDGRVVKAFIRFLD
                     RDHSMPNEPFVAQPVPYIGERVVVEAR"
     gene            690..1616
                     /gene="ftsY"
                     /locus_tag="VNR65_00920"
     CDS             690..1616
                     /gene="ftsY"
                     /locus_tag="VNR65_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWJ17265.1"
                     /translation="MNEPEKKSLFDRMKQAVSRTRETLEERIEGVIALTREVDENTLD
                     ELELTLIASDLGVTTTSEILDTLRDHAKRQAIRDGADLRQLLKTEIKAILDAQDQPRR
                     PVVGPEIILLVGVNGTGKTTTSGKLAALYRAQGKSVLLCAADTFRAAAIEQLEVWSQR
                     SGVELIKSKQGGDPTAVLFDALEAAKNRNFDIVIVDTAGRLHTKGGLMAELDKMRRTT
                     KRFFQDAPHEVLLVMDATTGQNGLQQARLFTESAGVTGIVLTKLDGTAKGGIAVAIAR
                     ELSLPVRYVGVGEKIEDLLPFDSEAFAESLLG"
     gene            1828..2994
                     /gene="ribD"
                     /locus_tag="VNR65_00925"
     CDS             1828..2994
                     /gene="ribD"
                     /locus_tag="VNR65_00925"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWJ17266.1"
                     /translation="MKSWSKPPVEKSPDLTEEAWMDQALDQARLGIGLASPNPTVGCV
                     IVKDGQLLGMGYHIYENRDHAEIVALRQAGLRSRGATAFVTLEPCSHYGRTGPCANAL
                     ITAGIARVVVATTDPNLDINGRGLAILQAAGIEIKLGVRRIEAQRLNDAFARFIRTGQ
                     PLVTLKAGLSLDGRIAPAFGVAPLGDPYWISGEESRAEVHRMRHAADAILLGINSVLI
                     DDPLLTDRSKLPRRRPLMRAILDSQLRIPLDSRLVCSAAEDLVVFCTTRGSADKQARV
                     HERIQVLEDRGVHVVELGADAHVPLTGALNWFASQKRTSVLLEGGSTIITQALREDLV
                     DKISLFYAPKILGGEAVPLLGDLTGHKVELQDVHVSRFGEDIRVQALLRDPWKF"
     gene            3089..3427
                     /locus_tag="VNR65_00930"
     CDS             3089..3427
                     /locus_tag="VNR65_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWJ17267.1"
                     /translation="MERTPDTGELIQGTLEMLVLKALARGPLHGYGVAEWIQQTSQQV
                     LKVEEGALYPALHRLELRGLLKAEWGASDNNRRAKFYALTAEGRKRLNAESQRWARLS
                     AAVAFVMQAP"
BASE COUNT          765 a         1247 c          936 g          639 t
ORIGIN      
        1 cactctgaac gcgtcgctga atactccgtc gccatcgccc aggaactcgg catggacaag
       61 gaagagatcg agcgtgtcca cctcgccgcc ctggtccacg acatcggaat gatcagcgtc
      121 ggagatgcct ccctgaagaa aaaccgcctg ctcgccgtgc gcgagttcga aatcctgcgg
      181 gcgcatcctg cccgtggcgc cgccatcttg cgctccatcg agtctctcca cgaccttatc
      241 ccagccgtgg aactgcacca cgaggcgctc gacggcaccg gctaccccta cggcctcaac
      301 gacaaggaca tcccgctgat ggctcgaatc ctggccgtgg ccgacacctt cgactccatg
      361 atttgcgctg acccattcca gcccggcctc acgcccgccg acgccgtcga cgcgctggaa
      421 gacctggcag gcacccgtct cgatggacgt gtcgtgaagg cattcatccg ctttctcgac
      481 cgagatcact ccatgcccaa cgaacccttc gttgctcaac cagtccccta catcggagaa
      541 agggtcgtcg tagaagcgcg ctaaacctcg taaatgcgct aaacagcgct cttccagcct
      601 ctttttgaag ccgtctgccc ctcttgatac actcaagttg aacgctcaag aggggcctcg
      661 ccggaatgat tcaaacactc tttggaagct tgaacgagcc ggaaaagaaa tccctcttcg
      721 accgcatgaa gcaggccgtc tcccgtacgc gggagaccct ggaagagcgc atcgaaggcg
      781 tcatcgccct cacccgcgag gtcgacgaga acaccctcga cgagttggaa ctcaccctca
      841 tcgcctccga cctcggcgtc accaccacca gcgaaatcct tgacaccctc cgcgatcacg
      901 ccaagcgtca ggccattcgc gacggggcgg atctccgcca gctcctcaag accgaaatca
      961 aggccatact cgacgcccag gaccagcccc gccgccccgt cgtcggaccg gagatcattc
     1021 ttctcgtcgg cgtcaatggc accggcaaaa ccaccacctc aggcaagctg gccgcgctat
     1081 atcgcgccca gggcaagagc gtccttctct gcgcagcaga taccttccgc gccgctgcca
     1141 tcgagcagct tgaagtctgg agccagcgca gcggcgtaga actcatcaaa agcaagcagg
     1201 gcggtgaccc caccgcagtc ctcttcgacg ccctcgaggc cgccaaaaat cgcaacttcg
     1261 atatcgtcat cgtagacacc gccggaagac tccacaccaa aggcggcctc atggctgaac
     1321 tcgacaagat gcgccgcacc accaagcgct tcttccagga cgctccccac gaagtactcc
     1381 tcgtcatgga cgcaaccact ggccagaacg gcctgcagca ggcccggctc ttcaccgaat
     1441 ctgccggagt caccggcatc gttctcacca agctcgacgg cacagccaag ggcggcatcg
     1501 ccgtcgccat cgcccgggaa ttaagcctcc cggtgcgcta cgtcggcgtc ggcgaaaaaa
     1561 tcgaagacct gctccccttt gacagcgaag ccttcgcgga atctctactc ggctgacccg
     1621 gtacgccgca acaacagtaa atatgtgact tcgattgcgc atgtcatctg taactctatt
     1681 tgcatctatt tacatgtaac ttatttcgca tgcgaaaaat gtccattcag tggtgatgtg
     1741 tgcagtcgct gaagaacttc ttgccgcgta ggctgagacg cactggtagc attcaccgtc
     1801 aatacatgaa agccgagtga ggcgcccttg aaatcttggt cgaaaccgcc agtggagaaa
     1861 agcccagacc tcacggaaga ggcctggatg gatcaggcgc tcgaccaggc ccgactgggg
     1921 ataggcctcg cctcgcccaa tcccacagtg ggatgtgtca tcgtcaaaga cggccagttg
     1981 ctcggcatgg gctaccacat ctacgagaac cgcgaccatg ccgagatcgt cgccctgcgc
     2041 caggccggac tacggtcccg cggtgccacc gccttcgtca cgctcgaacc ttgcagccac
     2101 tacggccgca ccgggccttg cgccaatgcg ctcatcaccg ccggaatcgc ccgcgtcgtc
     2161 gtcgctacta ccgatcccaa tctcgatatc aatggccgcg gtcttgccat cctgcaggcc
     2221 gccggtatcg agatcaagct cggcgtgcgg cgaattgagg cccagcgact taacgacgca
     2281 ttcgcgcgct tcatccgcac cggtcagccc ctggtcacac tcaaagccgg actcagcctc
     2341 gacggtcgca tcgctcccgc cttcggagta gctcccctgg gcgatcccta ttggatcagc
     2401 ggcgaagagt cccgcgctga ggtccaccgc atgcgccacg cagccgatgc catcctgcta
     2461 ggcattaaca gcgtgctcat cgatgacccg ctgctcaccg accgtagcaa actcccccgg
     2521 cggcgtccgc tcatgcgcgc cattctcgac tcacagttga gaatccccct cgactcccgc
     2581 ctggtctgct ccgctgccga agacctcgtc gtcttctgca cgaccagagg atctgcggat
     2641 aagcaggctc gcgtccacga acgcatccag gtcctcgaag accgcggcgt acatgttgtg
     2701 gagctgggag ctgatgccca cgtccccctc accggcgcgc tcaactggtt cgcttcacag
     2761 aagcgcacca gcgtcctcct cgagggcggc tcaaccatca ttacccaggc gcttcgcgaa
     2821 gacctcgtcg acaaaatcag cctcttctac gcgccaaaaa ttctcggagg tgaggcagtc
     2881 ccactgctcg gcgacctcac cggacataag gttgagttgc aggatgtgca cgtctcgcgc
     2941 ttcggcgaag acatccgcgt acaggccctc ctccgcgacc cctggaaatt ctaagccgtc
     3001 ccccgtccgc ctctataagc cccacaaact tcactctcgc ctcttgacaa gcccacggta
     3061 agaacgctac cgttccccta agatgattat ggaaagaact ccggacacag gcgaactcat
     3121 acaaggcacg ctggaaatgc ttgtcctgaa agccctggca cgtgggccac tgcacggcta
     3181 cggcgtggcc gagtggattc agcagacctc tcagcaggtc ctcaaggtgg aagaaggcgc
     3241 gctctatcca gcgctccacc ggctggaact tcgcgggctt ctgaaggccg agtggggcgc
     3301 atccgacaac aaccggcgcg cgaaattcta cgcgctcaca gcagagggaa gaaagcgcct
     3361 caatgcggag tcgcagcgct gggcaaggct ttcggccgca gtcgcatttg tcatgcaggc
     3421 accgtaatac atcgcctttg aggtaaaaag aatggctccc aacctccacg aatctctcct
     3481 tcgcctcaag gcactcttca gcagaaagcg cctcgatcgc gagatgctcg cagaactcga
     3541 gttccaccag gagatgctcc gcgaacggct cttgcgcgat ggcgttc
//
LOCUS       DATMGX010000058         2142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_405779, whole genome shotgun sequence.
ACCESSION   DATMGX010000058 DATMGX010000000
VERSION     DATMGX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405779"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VNR65_00935"
     CDS             <1..1211
                     /locus_tag="VNR65_00935"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWJ17268.1"
                     /translation="DAEGIEKLEHEVDEEVRLAAERAIHAPLPEIASIPRHIYSEDWD
                     PLSKDLNVEPATTDDIHERTMADLINLCLHDEMRRDPRIIIYGEDVADCSREDALKDG
                     AKGKGGVFKLTAGLQCEFGADRVFNSPLAEAAIVGRAVGYGVRGMKPVVEVQFFDYIW
                     PAVHQLRNEMSLMRWRSNGTFCSPAVVRVAIGGYLTGGSIYHSQSGESIFTHTPGLRV
                     VYPSNALDANGLLRTAIRCDDPVIFLEHKRLYRETYGRAVYPGPDYTIPFGKASIVRP
                     GTDVTLITYGAVVPRALQAALKAHRTEGIEVEVIDLRTLNPYDWEAIATSVRKTSRAI
                     VAYEDMLSWGYGAEIAARIGEELFEYLDAPVKRVAAMDTFVAYQPLLEDAILPQPEDL
                     YQAMLSLKHY"
     gene            complement(1284..>2142)
                     /locus_tag="VNR65_00940"
     CDS             complement(1284..>2142)
                     /locus_tag="VNR65_00940"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWJ17269.1"
                     /translation="EAFTPAIDTTNETTGLPGEINIDGKQSIPARKDISGITIADQLV
                     KAGFYWKSYQESLPPTGADLVNVSDGFFTNNTDFSQITPVLKPPLTSADLVYLYAVKH
                     NPFAYFASVQDGTDARNSFKNIVGFGGPHGLFADLSDGKAPTLAFIAPNQCNDQHGRG
                     NAGAFCNYDPNDDGTQAGLNPALIYQGDVTVRNLVVAIKASPAWKQGRSAIVVVWDEN
                     DYSISPTTNQVLVIVDTNYGAHGVRSNHRYTHYSLTKTLDAAFGLPCLNHACDDDVDA
                     MSDLFAEKQ"
BASE COUNT          437 a          719 c          572 g          414 t
ORIGIN      
        1 tcgacgccga gggaatcgag aagctcgagc atgaagtcga cgaagaagtt cgccttgctg
       61 ccgagcgcgc catccacgcg cccctgccgg agatagcgtc cattccgcgt cacatctact
      121 ccgaagactg ggatcccctc agcaaagact tgaacgtcga gcccgcgacc accgacgaca
      181 tccacgagcg caccatggcg gacctcatca acctctgcct ccacgacgag atgcgccgcg
      241 atccccgcat catcatctac ggcgaagacg tcgccgactg cagccgcgaa gacgcgctta
      301 aagatggagc caagggcaag ggcggagttt tcaagctcac cgccggattg cagtgcgaat
      361 tcggtgccga ccgggtcttc aattctcccc tcgccgaggc tgccatcgtc ggccgcgcgg
      421 taggctacgg cgtgcgcgga atgaagcccg tcgtcgaggt gcagttcttc gactacattt
      481 ggcctgcggt ccaccagcta cgcaacgaga tgagcctcat gcgctggcgc tccaacggaa
      541 ccttctgctc gccggcagtc gtccgcgtcg cgatcggcgg ctatctcacc ggaggctcca
      601 tctatcactc gcaatctggc gaaagcatct tcacccatac gccggggctg cgtgtcgtct
      661 acccatccaa cgccctcgac gccaacggac tgttgcgaac cgccatccgc tgcgacgacc
      721 cggtaatctt cctcgagcac aagcgcctct accgcgaaac ctacggacgc gccgtctatc
      781 ccggacctga ctacactatc cccttcggca aggcgagcat tgttcgcccc ggcaccgacg
      841 tcactctcat cacctacggg gcagtggttc cccgcgcgct gcaggccgcc ctcaaagccc
      901 accgcaccga aggcatcgag gtcgaggtca tcgatctgcg caccctcaat ccttatgact
      961 gggaagccat cgccacctcc gtcagaaaga ccagtcgcgc catcgtcgcc tacgaagaca
     1021 tgctcagctg gggctacggc gctgagatcg cagcacgcat cggagaagaa ctcttcgagt
     1081 acctcgacgc tcccgtaaaa cgcgtcgccg ccatggacac cttcgtcgcc taccagccgc
     1141 tcctcgaaga cgccattctt ccgcaacccg aggatctcta ccaggcgatg ctttcactga
     1201 agcactacta aatgccattg ccatccacaa acacaaaggg caccccgagc acatcgcagg
     1261 ggcgcccttt ttagcagaac tacttattgt ttttcagcaa acaagtccga catcgcatcg
     1321 acatcatcgt cgcatgcgtg attcaaacac ggcaggccaa aggccgcgtc cagcgtcttg
     1381 gtcagtgagt agtgcgtgta gcgatggttg ctgcgcacgc cgtgcgcacc gtagttcgtg
     1441 tccacaatca ccagcacctg gttcgtcgtg ggagagatgc tgtagtcatt ctcgtcccat
     1501 acaacaacaa tcgcgctgcg gccctgcttc catgcgggcg aggccttgat cgctaccacc
     1561 aggttgcgca ccgtgacgtc accctggtaa atgagcgcag gattcaagcc cgcctgcgtt
     1621 ccgtcgtcgt taggatcata gttgcagaat gctcccgcat tgccgcgccc atgctgatcg
     1681 ttgcactggt tcggcgcaat gaaggccagc gtgggagcct tgccgtcgct cagatcagca
     1741 aacagtccgt gcggtccgcc aaacccgacg atgttcttga agctgttcct cgcatccgtg
     1801 ccatcctgca cgctcgcgaa atatgcaaac ggattgtgct tcaccgcata cagatacacc
     1861 aggtctgctg aggtaagcgg cggcttcagc acaggcgtga tctggctaaa gtcggtattg
     1921 ttcgtaaaaa agccgtcgct cacgttgacc agatccgcac ccgtcggcgg caggctctcc
     1981 tggtagctct tccagtagaa gccggctttc accaactgat ccgcgatggt gatgccgctg
     2041 atgtccttgc gcgccggaat cgactgcttg ccatcaatat tgatctcccc cggcaggccg
     2101 gtggtctcgt tcgtcgtatc gattgccgga gtgaaggcct cg
//
LOCUS       DATMGX010000059         5564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_401164, whole genome shotgun sequence.
ACCESSION   DATMGX010000059 DATMGX010000000
VERSION     DATMGX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401164"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VNR65_00945"
     CDS             complement(<1..858)
                     /locus_tag="VNR65_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ17270.1"
                     /translation="MADADSVLEEPAAETHSFQAEVAELLRLMVHSVYTETDIFLREL
                     ISNASDACDRLRYEAIATPGLTSDGAPFVIRISPDKDKGTLTISDNGIGMGRQELIDN
                     LGTVARSGTRAFMSKLAEAKDGAGLIGQFGVGFYSAFMVADRIEVVSRHAGANEAWAW
                     TSSGGGGFEIATASEEQAARVSRGTEIVLHLKEDAKKYLETHEIERVVSTYSDHILFP
                     IELVDAEGKSEQINSASALWQRPKSELKPKDYAEAYRSIAHAFDEPAMTLHYRAEGRL
                     SYAVLLFAPS"
     gene            1099..1740
                     /locus_tag="VNR65_00950"
     CDS             1099..1740
                     /locus_tag="VNR65_00950"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWJ17271.1"
                     /translation="MAREAIIQEDTIIKGKIRNCRQMEIHGFVEGELAAESVLVHQGG
                     TFYGTLKTDQADVAGSVQGEVFVKNLISIRSSGSVNGHVRYGQLAMEMGADLSAELRN
                     VPPRLAGDLDITVKRGKSVIITTEDITAVDPDDSPDHLTYTVSNSRNGFASLAGQAGT
                     PIANFTQADIEAGRVLFVHDGGDSTTASFDVVVEDDSGATSGGSQTVNVHVKD"
     gene            complement(1794..2576)
                     /gene="hisN"
                     /locus_tag="VNR65_00955"
     CDS             complement(1794..2576)
                     /gene="hisN"
                     /locus_tag="VNR65_00955"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HWJ17272.1"
                     /translation="MTTSLERLLDTAHKLANAASEVTLSHFRKNAAADHKGGGLFDPV
                     TAADKGAETVMRDILARDFPDHGIVGEEFGTVNAGADYVWTLDPIDGTRAFIIGMPLW
                     GTLIGLQHHGKPLLGVMDQPFIGERFWNDDRAAWYRGPRGLQRCETRRCAGLDQALLT
                     ATTPDMFDGEDDVRFRRLAKAVRMRRFGGDCYSYCMLALGQIDIVAEAGLKPFDIVPL
                     IPIVEKAGGIVCSWDGGPAKDSGRCIACGDPTLLEPALALLK"
     gene            complement(2833..3729)
                     /locus_tag="VNR65_00960"
     CDS             complement(2833..3729)
                     /locus_tag="VNR65_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HWJ17273.1"
                     /translation="MSHEEAQCIAAELDPPFSIIGAQNQRVPIVFNSPHSGRIYPSVF
                     VESSRLSAQALRKSEDAYVDELLATAPAQGAVLMQAHFPRAYIDINREPYELDPVLFN
                     GRLPDFVNSQSLRAIGGLGTIARIVNEKEEIYHGPLTLETAFVRIEQLYKPYHAALHD
                     LMQSTRAQFGTAFLLDCHSMPSQQSDRGSFPDFVLGDRFGTSCAPEITKLVQTLLRSL
                     GYRVALNKPYAGGYITEHYGKPQQGIHAIQIEVDRSLYMNEETFEKSTAFSGLQWDME
                     RLIGMVVQELPRWTGGYKVAAE"
     gene            3895..5352
                     /locus_tag="VNR65_00965"
     CDS             3895..5352
                     /locus_tag="VNR65_00965"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HWJ17274.1"
                     /translation="MMKPASHKPAALVWFRRDLRLADHAALTAAFESGLAIIPVYILD
                     DDTPGEWRMGGASRWWLAQSLRALEKSLQRLGSHLILRRGKTEHVLRDLIAETGATAI
                     YFTRGYEPFQGTLEERLKWALGRSAQLRRFGGQVLFEPEKIVNASGDPFRVYSPFYRA
                     LTRREPPNAPLAAPPALPSPSSWPRSENLDDWGLEPTKPDWAGGIKAAWTPGEMAAYA
                     RLRLFIDRAVSQYRDQRNNPGSDATSRLSPYLAFGEISPRQVWHAVSTAAEAAGQSDM
                     GEAYLREIAWREFSYHLLFHFPELPQKAFRPEFENFPFREDKHALSAWQQGQTGYPIV
                     DAGMRQLWQTGWMHNRVRMIVASFLIKHLLQPWQSGEAWFWDTLVDADLASNSASWQW
                     VAGSGADAAPYFRIFNPVLQGEKFDPEGDYVRAFCPELAKLPASSIHKPWEGESDLLA
                     RCEVKLGVNYPHPMVAHAQARARALLAFEGIKKQI"
     gene            5427..5549
                     /locus_tag="VNR65_00970"
     CDS             5427..5549
                     /locus_tag="VNR65_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17275.1"
                     /translation="MRNFTLGALVVALVAFAGFYFYKQDHVTIEKPTLPKVETH"
BASE COUNT         1120 a         1673 c         1649 g         1122 t
ORIGIN      
        1 cgagggcgcg aacaagagaa ccgcatagga tagccggccc tccgcccggt aatgcagcgt
       61 catcgccggc tcgtcgaagg catgggcgat cgagcggtag gcctcggcat agtccttggg
      121 cttcagctcg gatttcggcc gctgccacaa tgcgctcgcg gagttgatct gctcggactt
      181 gccctcggca tcgacgagtt cgatcgggaa gaggatgtga tccgaatagg tcgagaccac
      241 gcgctcgatc tcgtgcgtct caagatattt cttcgcgtcc tctttcaggt gaagcacgat
      301 ctcggtgcca cgggatactc tcgccgcctg ctcttcgctc gctgttgcga tctcgaagcc
      361 gccgccccct gaggaggtcc aggcccaagc ctcattcgcg ccagcgtgcc ggcttacgac
      421 ctcgatgcgg tccgccacca tgaaagccga gtaaaacccg accccgaatt ggccgatcag
      481 ccccgctcca tccttcgctt ctgcgagctt gctcatgaaa gcgcgggtgc cggaccgggc
      541 gacggtgccg agattgtcga tgagttcctg ccgtcccatg ccgatgccgt tgtcggagat
      601 ggtgagcgtg cctttgtctt tgtcgggtga gatgcggatg acaaagggag cgccgtccga
      661 ggtcagtcct ggggttgcga tcgcctcgta gcgcagccgg tcgcaggcat ccgaggcgtt
      721 ggaaatcagc tcgcggagga aaatgtcggt ttcggtgtat acggaatgca ccatcagccg
      781 aagcaattcg gcaacctcag cctggaacga atgcgtctcc gcggcgggtt cttctaggac
      841 ggaatcagcg tctgccatct gtgtgctcag aatccggaat agctaactgg attggaatgg
      901 ctcccgatat aacaccctga ctgcgtcctg caaggtgctt gtccgcggac ggacagaagc
      961 ccctggtttt tggtgctcat tttctccaca ggccggattt cgattgcagc attccggcac
     1021 cgccgcctat ggtgcatgca accggggcgc ctcgcgctga cggcaccgcg atcaacagct
     1081 tgtaatgggg caaggcaagt ggcacgcgaa gcgattatcc aagaagatac aatcatcaaa
     1141 ggcaagatcc gtaattgccg ccagatggaa attcatggct ttgtcgaggg tgagcttgct
     1201 gccgagtcag tcctggtcca tcaggggggc accttctacg gaacgctgaa aaccgaccag
     1261 gctgacgtcg ccggttccgt tcaaggcgaa gtttttgtca agaacctgat cagcattcgc
     1321 agttccggtt ccgtgaacgg tcatgtgcgc tatggccagc ttgccatgga aatgggcgcc
     1381 gacctgagcg cggagcttcg caatgtgccg ccaaggcttg ccggcgacct cgacatcacg
     1441 gtcaaacgag gcaagtccgt catcatcacc accgaagaca tcaccgctgt cgatcccgac
     1501 gattcgcccg atcacctcac ctatacggtg agcaactcgc gcaacggatt tgcttctctt
     1561 gcgggccagg cagggacgcc gattgcgaat tttacccagg ctgacatcga ggccggccgg
     1621 gtgcttttcg ttcacgatgg cggagacagc accaccgcga gcttcgatgt tgttgtcgag
     1681 gatgacagcg gggcaacctc cggcgggtcc cagaccgtta acgtgcacgt caaggactga
     1741 cggacgagaa agcgccttct caagcgggga tcagagagat agcccgcttt acactacttc
     1801 aacagagcga gcgccggttc gagcagcgtt ggatcgccgc aagcgatgca acgcccggaa
     1861 tccttggccg ggccgccgtc ccaactgcag acgatgcctc cagccttctc gacgatcgga
     1921 atgagcggca cgatgtcgaa aggcttgagg cccgcttccg cgacgatgtc gatctgcccc
     1981 aaggccagca tgcagtagga atagcaatcg ccgccgaacc ggcgcatgcg gacggctttg
     2041 gcaagacggc gaaagcgaac gtcatcctcg ccatcgaaca tgtccggcgt tgttgcggtg
     2101 agcagcgcct gatcgagccc agcgcaacgc cgcgtttcgc agcgctgcaa gcctctgggt
     2161 ccgcgatacc atgccgcgcg gtcgtcattc cagaaccgct cgccgataaa gggctggtcc
     2221 atgacgccga gcagaggctt gccgtggtgc tgcaggccaa tgagcgtgcc ccaaagcggc
     2281 atccctatga tgaaagcgcg cgttccatcg atggggtcca acgtccagac gtagtcggcg
     2341 ccggcattca cagtgccgaa ttcctctcca acgatgccat gatcgggaaa gtcgcgcgcc
     2401 aggatatcgc gcatgacggt ttccgcgccc ttgtccgcgg cagtcacggg atcgaacagg
     2461 cccccaccct tgtggtcggc cgcggcgttc ttccggaaat gggacagcgt cacctcactc
     2521 gccgcattcg caagtttgtg agccgtatcg agcagccttt ccaatgatgt ggtcactgcg
     2581 ttccccattc gttccaaaga agcacaaatt cattttaaaa aagtcgaaag tttcccacag
     2641 gctaggaggc gattttgttg cgtttcggaa ccaattgtcg ctccacgagc ctattcacga
     2701 atgagtggct tgtcatttgt gcggtgcggt atgcgtcttt gatgcgtatc gctgccctcc
     2761 ttgggcgttt cctccctaga cttggggccg ttcaatcatc ttgaatggcc ctcttttttt
     2821 gcgcatttgg agctactccg cggccacttt gtagccgccg gtccagcgag gcaattcctg
     2881 gacgaccatg ccgatcaggc gctccatatc ccactgcaag ccggaaaaag cggtcgactt
     2941 ctcgaatgtc tcctcgttca tataaaggct gcggtcgact tcgatctgaa tcgcatggat
     3001 accttgctgc ggcttgccat agtgctccgt aatgtagccg ccagcataag gcttgttcag
     3061 agcgacgcga tagccgagac tccgcaaaag cgtctggaca agctttgtga tctctggagc
     3121 gcaggacgtg ccgaaccggt cgcccagcac aaaatccggg aagctgcccc ggtctgactg
     3181 ttgggacggc atcgaatggc agtcgagcag aaacgccgtg ccaaactgcg cgcgggtcga
     3241 ttgcatgaga tcatgaagcg ccgcgtgata cggcttgtaa agctgctcga tgcgcacgaa
     3301 cgcagtctca agcgtcagag gtccgtggta aatctcttcc ttttcgttga cgatgcgcgc
     3361 aatcgttcca agcccgccaa tcgcccgaag cgactggctg tttacaaaat ccggaagccg
     3421 gccgttgaac agaacggggt cgagttcgta gggctcgcga ttgatgtcga tataggcgcg
     3481 ggggaaatga gcctgcatga gcacagcgcc ttgagcgggc gcggtagcca aaagctcatc
     3541 cacataggca tcctccgatt tgcgcagcgc ttgagccgat agccgggagg actccacgaa
     3601 gacagagggg taaatccgac cgctatgcgg cgaattgaat acgatcggca cgcgctgatt
     3661 ctgcgcgccg attatcgaga acggcggatc gagctccgcc gcaatgcact gagcttcttc
     3721 gtgtgacata ggaggtgtcg cgagggcctt ccggcctgaa ccgatttgct ctcggtactg
     3781 tctcaaaaac cgcgcgctcg cgcaattgcg atctatcggc accctctcac gtttatggtt
     3841 tggaaggcgg aaggctttcc tgcaaaacgt ccaaaaaaat gcggtagcga tgtcatgatg
     3901 aaaccggctt ctcacaaacc cgccgccctt gtctggttcc ggcgcgacct aaggcttgcg
     3961 gatcacgctg cgctcacggc cgcctttgag tccggccttg cgattattcc ggtctatatc
     4021 cttgatgatg acacacccgg ggaatggcgc atgggcggcg caagccgatg gtggctcgcc
     4081 caatcgctgc gggcgctgga gaaaagcctc cagagacttg gatcgcacct gatcctgcgg
     4141 cggggtaaaa cggaacacgt gctgcgcgat ttgatcgcgg agacgggcgc gactgcgatc
     4201 tacttcacgc ggggctatga gcccttccag gggacgcttg aagaacggtt gaaatgggcg
     4261 ctcggacgtt ccgcacagtt gcggcgcttc ggcggccaag tcctgtttga gcccgagaaa
     4321 atcgtcaacg cgtcgggcga tcccttccgt gtttattcgc ctttctacag agcgttaacc
     4381 cggcgagagc cgccaaatgc gccgcttgcc gctcctccag ccctgccctc gccttcctcc
     4441 tggccgcgat cggagaacct tgacgattgg ggtctcgaac caacaaagcc tgactgggcg
     4501 ggcggcatca aagccgcctg gacgccgggt gagatggcag cctacgcgag gctgcgcctt
     4561 ttcatcgaca gggccgtgag ccaataccgc gaccagcgaa acaatcccgg cagcgatgcc
     4621 acctcccggc tgtcccctta cctcgccttc ggtgagataa gcccgcgcca ggtctggcat
     4681 gcagtatcaa ccgccgccga ggccgccggg caatccgata tgggcgaagc ctatctcagg
     4741 gagatcgctt ggcgcgagtt ctcctatcac ctgctctttc atttcccgga gttgccccag
     4801 aaagccttca ggcccgaatt cgaaaatttt cccttccgcg aggacaaaca cgctctcagc
     4861 gcctggcagc aggggcagac cggctacccg attgtcgacg caggcatgcg ccagctctgg
     4921 cagacaggct ggatgcacaa ccgcgtgcgc atgatcgtgg cctcgttcct gatcaagcac
     4981 ttactccagc cgtggcagtc aggcgaggcc tggttctggg atacacttgt ggacgcggat
     5041 ctcgcgagca actccgcgag ttggcagtgg gtcgcgggct cgggcgcgga tgcagcccct
     5101 tatttccgca tcttcaaccc cgtcctgcag ggcgagaagt tcgaccccga gggcgattat
     5161 gtgcgtgcgt tttgtccgga gctggcgaaa ttgcccgcct ccagcatcca caagccgtgg
     5221 gagggggaat cggacctctt ggcgcgttgt gaagtgaagc tcggcgtgaa ctatccccac
     5281 ccaatggtcg cccacgcgca agcgagagcg cgcgcgcttc tggcttttga aggcatcaag
     5341 aagcaaatct gaccacggaa caagagccgg aacgaaagcg gtttcgattg cgtttgctcc
     5401 ccatcgtttc gctgaaggag aacatcatgc gcaatttcac tctcggggcg ctcgtcgtag
     5461 cgctagtcgc ctttgcaggc ttctatttct ataagcagga ccacgtcacg atcgagaagc
     5521 ccacgttgcc gaaagtcgag acccattgat ccaaaaggcc ggga
//
LOCUS       DATMGX010000060         2613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_19116, whole genome shotgun sequence.
ACCESSION   DATMGX010000060 DATMGX010000000
VERSION     DATMGX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19116"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..409
                     /locus_tag="VNR65_00975"
     CDS             188..409
                     /locus_tag="VNR65_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17276.1"
                     /translation="MTLEQEILDAVRALPTDKQQEFLDHVKRLRDEATQPRKPRKSGR
                     GLWADLNIDLSAEVIDEARREMWKDFRQG"
     gene            498..>2613
                     /locus_tag="VNR65_00980"
     CDS             498..>2613
                     /locus_tag="VNR65_00980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWJ17277.1"
                     /translation="MLIEQQITERRERAAGAIAKILKRPMGEPYGDYSVKSASGKTYR
                     LAMRGPGLFENYCSCPDFRVNTLGTCKHIEALLLRLRRRHGAALDRKGYARTRSSISL
                     QYGETIEIRLRMPALPTPALRAIAEEYFDSAGLLRREHFPSFHRVREVFRHADQDAVI
                     YSDVLEYVDRENEISEGLEWERQLLGKLRRGQDPLNGVLKTKLLPYQTQGAIFAASRG
                     RVVLADDMGLGKTVQALAAAELLRRRRGIARVLVVAPASVKYQWKTEIEKFTDLSAQV
                     IEGSLPRRRALYASPTFFNLTSYELVLKDVRDIHEMAPDLIILDEAQRIRNWTTATAR
                     TVKQLKSRYAFVLSGTPLENKLEELFSVVEFIDGRRLGPAFRFLHEHRTEDEKGTLIG
                     YRGLDRIHEQLTPVLLRRTRGEVLKDLPHRTDQILHVSLTPQQAEPYWEQNDILAGLM
                     RKWERQGWLSEIDQKRVLCCLQNMRMLCNSTFLFDKQTNHSPKLAEFREIVRELVIEE
                     GRKVVVFSEYERMTHLAGEELKKLGIGFVSLHGGVPSRNRGALMERFRRDAECKVFLS
                     TDAGGVGLNLQAASAVINFEPPWNPARLEQRIGRVHRLGQAHPVHVVHLLTEDSIEER
                     VWETLKLKKSLFAGVFDSPTGEVSFAKLGRKSVLQTMKLKKSLFAGVFDSPTGEVSFE
                     ALGRKSTMQTLKEIFANQPDRPK"
BASE COUNT          556 a          743 c          820 g          494 t
ORIGIN      
        1 ccatggtggc gtcgtttgcg tctgtttgac aatccggaag tagttagggg cggctcttag
       61 gtctgaactg cttgaaacgc gtgatgggta gcgccggggc gaaaacgttt tccgacttcg
      121 cagtgctcgc cgacgaaggc tcactgaatg ccaaaacaac cgataatgtt ggcaggtagg
      181 gtttccgatg actctggaac aagagattct cgacgcggtc cgagcgcttc ccacagacaa
      241 gcagcaggag ttcttggatc acgtaaagcg ccttcgcgac gaggcaacgc aaccgcggaa
      301 gccccggaaa agcggacgcg gcctatgggc cgatctgaat atcgatttga gcgccgaggt
      361 gatcgacgag gcccggcggg aaatgtggaa ggattttcgt caaggatgac ttctgatacc
      421 atccgtcgtc gcggataccc gtctgccggc gccctgctat tctgactcgt tggtctgaag
      481 caggaggatt ctccggcatg ttgatcgagc agcagattac cgaacggcgg gaacgggcgg
      541 cgggcgcgat cgccaagatc ctgaagcggc ccatgggaga gccgtacggc gactatagcg
      601 tgaagtccgc cagcggaaaa acctaccggc tggcaatgcg cgggccaggg ctattcgaaa
      661 actactgctc gtgtccggac ttccgcgtca atactttggg aacgtgtaag cacattgaag
      721 cgctgcttct gcggcttcgg cgccggcacg gcgccgcgct cgaccgcaag ggctatgcgc
      781 gcacccgctc ttccatctcg ctgcaatatg gagagaccat tgagatccgg ctgcggatgc
      841 cggctttgcc gacgccggct ctgcgcgcca ttgccgaaga gtacttcgac tctgccggat
      901 tattgcggcg cgaacacttt cccagctttc atcgggtgcg ggaggtgttc cgccatgcgg
      961 atcaggatgc ggtaatctac agcgacgttc tcgaatatgt ggatcgcgag aacgagattt
     1021 cggaaggact ggagtgggaa cggcaactgc tcgggaaact tcgccgtgga caagacccac
     1081 tgaatggagt gctcaagacc aaactgctgc cctaccagac gcagggcgcc atttttgccg
     1141 cctcccgagg ccgagtggtt ctggccgacg acatgggatt gggcaagacg gtgcaagcct
     1201 tggccgcggc ggaactgctg cggcgtcgcc gaggcatcgc gcgcgtgctg gtggtggccc
     1261 cggcttcggt gaagtatcag tggaagacgg aaatcgaaaa gttcacggac ctttcggcgc
     1321 aggtcatcga aggatcgctg ccgcggcgcc gcgcattgta cgcgtcaccc acgttcttca
     1381 accttacgag ttacgaactg gtcctcaaag acgtgcgcga catacacgag atggcgcccg
     1441 acctcatcat cctggatgag gcgcaacgca tccggaactg gaccaccgcc accgcccgca
     1501 cagtaaagca actgaaaagc cgctatgctt tcgtgctgag cggaacgccg ctcgagaata
     1561 agttggagga gctgttttcg gtggtggaat tcatcgacgg ccgccgtctc ggtccggcgt
     1621 tccgctttct ccacgagcac cgcacggagg atgaaaaggg aaccctgatc ggatatcgag
     1681 gtctggaccg cattcacgaa caactgacac cggtcctgtt gcggcggacg cggggagagg
     1741 tgctgaagga tctaccgcac cgcacggacc agattctcca cgtgtcgctg accccgcagc
     1801 aggcggaacc atattgggaa cagaatgata ttctggccgg tctgatgcgt aaatgggaac
     1861 ggcaaggctg gctctccgag atcgaccaga aacgtgtcct gtgctgcctg cagaatatgc
     1921 ggatgctctg caattccacg tttctcttcg acaagcaaac caaccattcg ccgaagctcg
     1981 ccgagtttcg cgagatcgtc cgcgagttgg tgatcgaaga aggccgcaag gtggtggtct
     2041 tcagcgagta cgaacgcatg acccacctgg ccggcgaaga gctcaagaag ctggggatcg
     2101 gattcgtctc tctgcacggc ggcgtgccct cgcgaaatcg gggcgcgctc atggagagat
     2161 tccggcgcga tgccgagtgc aaggtgtttc tctccaccga cgccggcggc gttgggctga
     2221 atcttcaagc ggcctcggcg gtgatcaatt ttgagccgcc atggaacccg gcgcgcctgg
     2281 agcagcgcat cggccgggtg caccgtctcg gccaggccca tccggttcac gtggttcacc
     2341 tgctgactga agacagtatc gaagagcgcg tgtgggaaac tctgaaactg aagaaatccc
     2401 tcttcgcggg cgtgttcgat tcgccaacgg gcgaggtcag cttcgcgaag ctgggacgca
     2461 agagcgtcct ccagacgatg aagctgaaga aatctctctt cgcgggcgtg ttcgattcgc
     2521 ccacgggcga ggtcagcttc gaggcgctag gaagaaagag caccatgcag acgctgaaag
     2581 agatcttcgc caatcaaccc gaccgtccaa aac
//
LOCUS       DATMGX010000061         3420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_156822, whole genome shotgun sequence.
ACCESSION   DATMGX010000061 DATMGX010000000
VERSION     DATMGX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156822"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VNR65_00985"
     CDS             <1..500
                     /locus_tag="VNR65_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWJ17278.1"
                     /translation="RFCDCFNIPLLTFVDVPGFLPGTAQEYGGLIKHGAKLLFAFAEA
                     TVPKVTVITRKAYGGAYDVMSSKHIRGDVNYAWPTAEIAVMGSKGAVEILYRSELGDK
                     DKIAARIKEYQDRFENPFIAAERGYIDEVIMPWNTRYRVARALNMLRNKQQDMPWKKH
                     SNIPL"
     gene            complement(778..1476)
                     /locus_tag="VNR65_00990"
     CDS             complement(778..1476)
                     /locus_tag="VNR65_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ17279.1"
                     /translation="MPDVLFEALCATLDHFDAGFVAVFPDGKILHANRMAHEMMAEGW
                     PIQANNGSLQACSKKATTLLLKSLRQVTQTTERSPSDDISLDICLADAASPKGVAIAT
                     MKPLLNTRLESSPVAIFVTSIESRGDCALAAIAECFGLTPAETRTLHHFVEGGTVANA
                     GHALAVSENTVKTHLQNIFAKTRSSRQAQLIKLINDLRPPLRQMANASGSGDNGSTLF
                     SGRFCPNHNTTVRL"
     gene            complement(1579..2352)
                     /locus_tag="VNR65_00995"
     CDS             complement(1579..2352)
                     /locus_tag="VNR65_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWJ17280.1"
                     /translation="MQAKTDPYRNVSPGPRAGVLDPAKMPDHRTPIFKRFEVLKNLPA
                     DALDDLAGALSWTSAPAGKQIVLANEPCGDVYFIVDGRVKVLLYSAAEGKPVLFVTLR
                     AFEMFGEMAAIDGSPRSATVEAEDDCVLAVLSREQFLRLIRAYPAFSFAVMKQLVAQV
                     RRLSDRVYEFSTLGVQARVYAELLRVVALAGEQNGQALLSPAPLLVDLAARVSTHREA
                     VSRVISRLQDNGILRREGANIRVLEIDRLRRLLREEKGE"
     gene            2581..2976
                     /locus_tag="VNR65_01000"
     CDS             2581..2976
                     /locus_tag="VNR65_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17281.1"
                     /translation="MAEEERFERWEPIDGLSSKMYIDGMYDDREGFRLILKSTEPKMR
                     SLRILFDAPLCYRGAQEVYLLRQIYSENDIYPWPIFIVRNSRYAKWFYSQAKETIDKD
                     GHHYHIAAMDEIIDVLSARPPIVTWLNDI"
     gene            3215..>3420
                     /locus_tag="VNR65_01005"
     CDS             3215..>3420
                     /locus_tag="VNR65_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17282.1"
                     /translation="MKFKLLGAFLMTLALSSAATAQTLTPSKLAFHGKLENGLEIVVV
                     PDRRAPVVTHMVWYKVGGADEPSG"
BASE COUNT          765 a          939 c          957 g          759 t
ORIGIN      
        1 tccgcttctg cgattgcttc aacatcccgc ttctcacctt tgtcgatgtg ccaggcttcc
       61 tccccggaac ggcccaggaa tatggcggcc ttatcaagca tggcgcgaag cttctcttcg
      121 ccttcgcgga ggcgaccgtg ccaaaggtga cggttatcac ccgcaaggcc tatggcgggg
      181 cttatgacgt catgagttcg aagcacatca gaggcgacgt caactatgcc tggccgacag
      241 ccgagatagc ggtgatgggc tcgaaagggg cagtggaaat cctctatcgg tcggaactcg
      301 gcgataagga taagatcgcc gcccgcatca aggagtatca ggaccggttc gaaaacccct
      361 tcatcgcggc ggaacgcggg tatatcgacg aggtgatcat gccgtggaac acgcgttacc
      421 gtgtggctcg cgcgctgaat atgctgcgaa acaagcagca ggatatgccg tggaagaagc
      481 atagcaacat cccgctatag ggatgttgta aagctgcctg tttggacggc tctcgcacct
      541 tagaatcgat caaccattcg gcctttaacc gattccgatt aaaggcgcgt tgatctaaag
      601 cgcgtcgaaa ttattcggaa tccccttatt gtcatggccg ccctcttagg cggccatcca
      661 ggctaacata ttccatcttt ttggatcttg aggctggatg gccgggtcaa gcccggccat
      721 gacatcggtt ccagctaatc gcgacacgct ataggcgcga cctgtttacg gccgtagcta
      781 cagcctcacc gtggtgttat ggtttgggca aaaccggccg gagaacaggg tcgatccatt
      841 gtcgccggag ccgctcgcat ttgccatctg cctcaaaggc ggtctgagat cgttgataag
      901 tttgatgagt tgcgcttgcc gcgaggatct cgtcttggcg aagatgttct gcagatgggt
      961 cttgaccgta ttttccgaga cggccagcgc atgaccagca ttggcaaccg tgcccccctc
     1021 cacaaaatga tgcagggttc tcgtttcggc gggcgtcaat ccgaagcact cggcaatggc
     1081 agccaatgcg caatcgccgc ggctttcgat gctggttacg aaaatcgcga cgggagaaga
     1141 ctcgagccgc gtgttgagga gcggcttcat cgtggcaatc gctacgccct tgggcgaggc
     1201 agcgtctgca agacagatgt ccaggctaat atcgtccgaa ggggagcgtt cggtcgtttg
     1261 cgtgacctgt ctcaaactct tcaaaaggag agtcgtcgct ttcttgctgc aggcctgcaa
     1321 ggaaccgttg ttcgcctgga tcggccagcc ctcggccatc atttcatgcg ccatccgatt
     1381 ggcgtgcagg atcttgccgt cgggaaaaac cgcaacaaat ccggcgtcga agtgatcaag
     1441 cgtggcacac aaggcttcga agagaacatc cggcatggcc gctctttcgt tgcgtacagg
     1501 cacaatgata cgaagccaga taagataatg gtaaagaaag gcaacagtgt tggcaaaact
     1561 cacgcgccgc taaagatttt attcaccctt ctcctcgcgt agcagccgac gcagccgatc
     1621 gatttccagg actcggatgt ttgcaccttc gcgcctcaga atcccgttgt cctggaggcg
     1681 gctgatgacg cgcgatacgg cttcccggtg cgtgctgacc cttgcggcga ggtccacaag
     1741 cagcggtgcc ggcgaaagaa gtgcctggcc gttttgttct cccgctagag ccacaacgcg
     1801 cagcagctcg gcataaacgc gcgcctggac gccgagggta ctgaattcat aaacccggtc
     1861 cgacaatcgc cggacttgag ccacgagttg cttcatcacc gcaaacgaaa aggccggata
     1921 agcgcggatg aggcgaagaa actgctccct ggacaaaacc gcgagtacgc aatcgtcttc
     1981 ggcctcaacc gtggcggaac gcgggctacc gtcaatggca gccatctcgc cgaacatctc
     2041 gaatgctcga agcgtgacaa aaagcactgg cttgccttcc gcggcggagt aaagaagcac
     2101 cttcaccctg ccgtccacaa tgaaatacac gtcgccacat ggctcgtttg cgagcacgat
     2161 ttgctttccc gcgggcgcgg atgtccagga aagggcgccc gccaggtcat cgagcgcatc
     2221 ggcaggaaga tttttcaaaa cctcaaagcg tttgaatatc ggtgtgcgat gatcgggcat
     2281 ttttgccggg tccagaacac ccgcacgcgg gccaggagat acgttgcggt aaggatccgt
     2341 ctttgcttgc acaaacctct tatattggca attagatttt ctgatgaggc acaaggtgta
     2401 ttttcatcaa attgcaattg aagcgcgcgg ggaactcgcc cgagcaagca ttcctttaca
     2461 aggcgcttga gcgatcgcat tctttgcatg tattccttgc gaggcctctc ctctatatta
     2521 taaggctgag gtattggttg ggtcgtatgt gttatagagt ttttagggcg atctccagac
     2581 atggctgagg aagagcggtt cgaacggtgg gaaccgatcg acggtttatc gtcgaaaatg
     2641 tatatcgacg gcatgtatga cgatcgggag ggctttcggc ttattctcaa atcgaccgag
     2701 cccaagatga ggtcgcttcg gattcttttc gatgcgcctc tctgctacag gggcgctcag
     2761 gaggtctacc tcctccgcca aatctacagc gagaatgaca tctatccctg gcctattttt
     2821 attgtgcgga attcccggta tgcgaagtgg ttctactcgc aggcgaagga aacgatcgac
     2881 aaggatggcc atcattatca tatcgcggcc atggacgaga tcatcgatgt gctgtccgcc
     2941 cgtcctccaa tcgtgacctg gctcaacgac atttgaggcc cgccgggaca gaaaaaaacc
     3001 ggcgagcggg cataaaatca tcgctttttg cgtgcagtgc tctcggagat tgttcccgag
     3061 gttcaatgaa atagccgtaa caattgtggc ggctgagctt cgctccccct atatcaccag
     3121 ttgcacttca cccaggatgg tcaaatcgcc gatgcaacac caattgttag aaacccgtcc
     3181 ccattcaagt tggttgggag tgggccgact actcgtgaaa ttcaaactgc ttggtgcgtt
     3241 cctcatgact ttagcactct cttcagcagc cacggctcag acactcacgc cttcgaagct
     3301 cgctttccac ggcaagctcg aaaacggact tgagatcgtc gtggtgccag atcggcgcgc
     3361 cccggtagtc acgcatatgg tgtggtacaa ggttggcggc gcagacgagc cttcgggcaa
//
LOCUS       DATMGX010000062         3151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_400914, whole genome shotgun sequence.
ACCESSION   DATMGX010000062 DATMGX010000000
VERSION     DATMGX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400914"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1636
                     /locus_tag="VNR65_01010"
     CDS             335..1636
                     /locus_tag="VNR65_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HWJ17283.1"
                     /translation="MENGIRYFFPGAILERVPQRPDQMPGLLGAVPSGGQVEFEWLGA
                     GYSLTVSSNLSDHERRLLRSIGMVLSARYQLITNQALAAQSFQLFRGLPEDRFVSAFL
                     DPSPHTSGEAIAGVPDRIADAIEVLRTSSMSTYENRRIATGVLLFGSMPEPCHEPPQT
                     PDWALRYSHELTTIRSFHRLCDGLQTLALVGRDGLLAELIDVHEWTEPYRDMPLPVPT
                     SARYRDHCRATLCGGHICLILSPNGEVKIYADGVPVFRFLDGKWRLVDARYRYEMWKN
                     AIGDTVLAERLFAVALNLTESRRGALFVVLDDPESARALVSQPDLLDHMNHEPMVEGI
                     SSKDQLHYLLRDKSIFDIPSTVLENIARIDGAVVLTSQANLLAFGAILHHQSSEEAIN
                     RPVEGGRTTAAIAASRHGTVLKVSEDGLISMYRGGCCLWEM"
     gene            1638..2435
                     /locus_tag="VNR65_01015"
     CDS             1638..2435
                     /locus_tag="VNR65_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17284.1"
                     /translation="MTEEHNLSSRAGPIQNLIRCSLWVGVLFLAVVAAAGAETDAAPV
                     PCPAGVNLAGDDVVARLMSRNAERARELLGFQATRHYQLHYTGFPSTLGAEMQVKVSY
                     MAPGTKEFTIESESGSKLILNRVFHRLLESEKESGSNEANRTAVALAPGNYRFSLLGC
                     VPGDGRSQYVMQVEPLHDTKYLYRGKVWIDTTDFAVTRIEAQPAKNPSFWTKRTEIHH
                     EYQRVGEFYLPVLNQTVTDVRLGGKAVLTIRYLDYKLSVAESLVDPR"
     gene            2635..>3151
                     /locus_tag="VNR65_01020"
     CDS             2635..>3151
                     /locus_tag="VNR65_01020"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HWJ17285.1"
                     /translation="MHTRKQPSTYQFDLSGGALCLDFANTVSHRHLPQRRAEHLDSYA
                     DLVAFAEQSKLLLPKLAASLRARSARNGAEARSSFRKSIVLREGLYRAFSAIAAGKHA
                     RPEDVQQINDFALEALKHRKLARTNGDYRWEWQSSESDLLDRILWPIAESAADLLTSE
                     DRAAIRECGAPD"
BASE COUNT          610 a          979 c          921 g          641 t
ORIGIN      
        1 aaggtcctcg tacatctccg cggtcagcgc gttcttcttc tctggacgat taatggtaat
       61 gcgccgtacg gcgccgttgt tttcgctttc aatgtggctc atggtgtcgt ccctgcttgg
      121 tgagttcgag aaacttaggg agcgcagata ctgccgcatc gcggtgaaaa aacattgtac
      181 gggaagcggc aagggtcgcg cacccgacgt gcctcgaggc cagattcagc ggttgatttg
      241 atgtaagctt taagcagtga tgaatccctc gcccattccg cttcgctcgc ccgaaaatcg
      301 tactcggttt atcagtggac tctacggcga cttgatggag aatggcattc gctatttctt
      361 tcctggagca atcctggaac gcgtgccgca gaggcccgat cagatgccgg gcctgctggg
      421 cgcggtgcct agcggaggcc aggtcgaatt cgagtggctg ggggcgggct actcgctgac
      481 cgtaagttcg aatctgtcgg accatgaacg ccggctgctg cgcagcatcg gcatggtgct
      541 ttccgcgcgc tatcaactga tcaccaacca ggcgctggcg gcgcaaagct ttcagctctt
      601 ccgcggttta cccgaggacc gctttgtctc ggcgttcctc gatccttctc cgcacaccag
      661 cggcgaagcc attgccggcg tgcccgatcg cattgccgat gccatcgaag tcttgcgcac
      721 cagcagcatg agcacctacg agaaccgccg cattgccacc ggagtgttgc tgttcggctc
      781 catgccagaa ccgtgccatg aaccgccgca gacgccggac tgggccctgc gctattccca
      841 cgagctgacc accatccgca gtttccatcg tttgtgcgat gggttgcaga ccctcgccct
      901 ggttggccgg gacggcctgc tggcggaatt gatcgacgtg cacgaatgga ccgagcccta
      961 tcgcgacatg ccgctgccgg tgccgacctc agcccgctac cgcgaccact gccgcgccac
     1021 tttgtgcggt ggacacatct gcctcatcct ctcccccaac ggcgaagtca aaatttacgc
     1081 cgatggtgtg cccgtcttcc gctttctcga cggcaagtgg cggttggtag atgcgcgcta
     1141 tcgttacgaa atgtggaaga acgccatcgg cgacaccgtg ctggcggagc gcctgtttgc
     1201 cgtggccctg aacctcaccg agagccgccg gggcgcgctg ttcgtggtgc tcgacgatcc
     1261 ggagtcggcg cgagcgctgg tgtcgcagcc cgatctgctc gaccacatga atcacgaacc
     1321 gatggtagaa ggaatttcca gcaaggacca gttgcattat ctgctgcgcg acaagagcat
     1381 cttcgacatt ccttccacgg tgctggagaa cattgcccgc atcgatggcg ccgtggtgct
     1441 cacttcccag gccaacctgc tggcctttgg cgccattctt caccatcagt ccagcgaaga
     1501 ggctatcaac cgccctgtcg aaggcggacg caccaccgcc gccatcgccg cttcgcggca
     1561 tgggaccgtc ctgaaagtca gcgaggacgg cttgatctcc atgtatcgcg gaggatgctg
     1621 cctgtgggag atgtaggttg accgaggaac acaatcttag ttcgagggcg ggccccattc
     1681 aaaacttgat ccgttgttcg ctgtgggttg gagtcctgtt tctcgccgtg gttgccgcag
     1741 ccggggctga gaccgatgcc gctcccgtac cctgcccagc cggggttaat cttgccggcg
     1801 acgatgtggt cgcgcgcctg atgagccgga acgcggagcg cgcgcgggag ttgctcggtt
     1861 tccaggcgac acgccactac caattgcact acaccgggtt tccttcgacg ctcggcgccg
     1921 agatgcaggt gaaggtctcc tacatggcgc ccggcaccaa ggagttcacg attgaatcgg
     1981 agtccggctc caagctcatc cttaaccgcg tcttccaccg gctgctggaa tcggaaaaag
     2041 aatccgggtc caacgaagcc aaccgtacgg cggtggccct tgcccccggc aactatcgtt
     2101 tctcgttgtt gggttgtgtg ccgggcgacg gccggtcaca gtacgttatg caggtcgagc
     2161 ccctgcacga cactaagtat ctgtaccgag ggaaggtgtg gatcgatacg acagactttg
     2221 cggtgacgcg tatcgaggcg caaccggcga agaacccgtc attctggacc aagcgcaccg
     2281 agatccatca cgagtatcaa agagtcggcg agttctacct gccggtgctt aaccagacgg
     2341 tcaccgatgt ccggctgggc ggcaaggccg tgctcaccat ccgctatctg gattacaagt
     2401 tgtcggtggc cgagagcctg gtggacccgc gctagagaaa ctctgaataa ctcgatgttc
     2461 ggggcagccc gaagggcgag acaaacttag cccaggacgt aagtcctggg taaatcggcc
     2521 atgaaaatat gagccccttc aggggcgaca ccgaaacgat agcggttctt gaaccggcca
     2581 taatttgtgt tcgcctaacc tgctatttgg catagacggg ttacaatacg agcagtgcat
     2641 acccgcaaac aaccctctac atatcagttc gacctaagcg gtggagcgct ttgcctcgat
     2701 ttcgccaaca ccgtaagcca tcgccatctt ccgcaacgcc gcgccgaaca ccttgacagc
     2761 tatgccgatc tggtggcgtt cgcggagcaa tcgaaactcc tcttgccgaa gctggccgcc
     2821 agcttgcggg cccgctcggc ccgcaacggc gcagaagccc gaagctcctt ccgcaaatcg
     2881 atcgtcctcc gcgaaggcct ctatcgcgct ttttccgcaa tcgccgcagg caagcacgcc
     2941 cgtcccgagg acgtccagca gatcaacgac tttgcactcg aggcgctgaa gcaccgcaag
     3001 ctagcgcgaa ccaacggtga ctatcgctgg gaatggcaat cgagcgaaag cgatcttctc
     3061 gatcgcattc tgtggcccat cgcggagtcc gctgccgatc tgcttacctc ggaagatcgc
     3121 gccgccattc gcgaatgcgg ggcccccgat t
//
LOCUS       DATMGX010000063         2784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_400689, whole genome shotgun sequence.
ACCESSION   DATMGX010000063 DATMGX010000000
VERSION     DATMGX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400689"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..>2784)
                     /locus_tag="VNR65_01025"
     CDS             complement(143..>2784)
                     /locus_tag="VNR65_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17286.1"
                     /translation="AHTDEGFWIAVLPFKSIGASPEVATLADGLGEEIVTGLSRFSYL
                     RVIARSSTSRYSQGGVDVRTAGHELGARYVMEGSLRQAGAKLRLSVQLVDGITGAHLW
                     AENYERAFSPDAFFELQDDLVPRIVSTVADTQGILPRTMSDILRTRNSSDLSPYEAVL
                     RSFAHFQRVNAEEHAASRAALERAVEVQPGYGDAWAMLSLICKEEFTHELNLRPDPLG
                     RAMAAALRAVEAAPSNHLAFHALAMANYFLGDRPAFRSAAERALELNPMDGFSIAFLG
                     SFIAYDGDWEYGCRLSAKARSLNPHYPGWYWFVPCFDAYRKGDYRLSLEFARKVNMPS
                     LWRASAIMAAALGQLGEIESARILLQTLLTRRPNFKAEARKDLAIFWGPDLVEGMIEG
                     LRKAGLEIADGQKPAPSRHPSQSGANLTAATPSLAVLPFANLSAEKDQEYFSDGLAEE
                     IINLLAQISGLKVIARTSAFAFRGKEEDIRGIADALGVTTILQGSVRRAGNRIRVTVQ
                     LINASDGAHLWSERYDSEMTDVFVVQDEIAAAIAGALQVKLSAPPREYVPQLAAYEEF
                     LKARHHLQRWTPESAARGRECLERAVVFDPGFALAHSELGWCYYILAIENQIPPGEAA
                     ESMRATARKALGIESSLPDAHAVLAMAGILDYDWSEAGRQFQLAMASERIQPFICYLH
                     SAFYLVSLGRMREAEQGVERVLQEDPLNALCRTALGSYRLASGRFAEGEAILRDALQL
                     DENFWIAQSWMGFSCLKQNRMTEAIAYEEKARSLAPWNPTVIGRLAGMHERVGEKSRA
                     QALLEELGSGTAFGVPGGFLCYHSVLADVDSAAGWYEKAIEQRDPRAPWLFPMQFGDL
                     LTSSPRWPGLMRRMNLPAAAGLS"
BASE COUNT          575 a          861 c          829 g          519 t
ORIGIN      
        1 ttggccggta cgcgaaccgt tagcgtacgt aaatttccct ttcttgaggt gtcccccaag
       61 tcgaggaatg cgtcacgcga ttgttgaccg atgagtaatt cctcagtaga cggtcataca
      121 ggcgcagtcc cttcatccgc aatcaagaca agcccgcagc ggcgggaaga ttcatccgtc
      181 tcatcaaacc tggccagcgc gggctagagg taaggaggtc gccaaactgc atgggaaaga
      241 gccacggcgc acggggatca cgctgctcga tggccttctc ataccagccg gcggcagaat
      301 cgacatccgc aagaaccgag tgataacaaa gaaagccacc cggaactcca aacgccgtgc
      361 cgctgccgag ctcttcgagc aaggcttgcg cgcggctctt ttctccgacg cgctcatgca
      421 ttccggcgag ccggccgatc accgtcggat tccagggagc cagagatcgt gccttctctt
      481 cgtaagcgat ggcttcggtc atccgattct gctttagaca agagaagccc atccatgatt
      541 gggcgatcca gaagttctcg tcgagctgca aagcatcgcg aagtatagct tcgccctccg
      601 cgaatctgcc ggatgccagg cggtagctgc ccaaagctgt gcggcacaaa gcgttgagtg
      661 gatcttcctg aagcacgcgc tcaaccccct gctcggcctc cctcatgcgc cctagagaca
      721 caagataaaa cgcactatgg aggtagcaaa taaacggttg aattcgctcg ctggccatgg
      781 cgagttggaa ctgccggccc gcctcggacc agtcgtaatc caggatgccc gccatcgcca
      841 ggacagcgtg agcatccggc agcgaggatt cgattcccag tgcttttcgt gccgtcgccc
      901 gcattgactc ggctgcctcg cccggaggga tctgattctc gatagccaga atgtagtagc
      961 accatcccag ctcgctatgt gccagtgcga atccaggatc gaacacaact gccctttcca
     1021 ggcattccct accgcgtgcc gccgactcgg gagtccagcg ttgcagatga tgccgggcct
     1081 tcaggaactc ctcatatgcc gccagctggg gcacgtactc tcgtgggggc gcggaaagct
     1141 tcacctgcaa cgccccggca atggccgcgg cgatttcgtc ctgcaccacg aacacatcgg
     1201 tcatctcgct gtcatagcgt tccgaccaga gatgcgcgcc gtcggaggcg ttgatgagct
     1261 gcaccgtcac gcgaatgcgg ttgccggcgc ggcgcacgct gccttgcagg atggtcgtta
     1321 cgccgagcgc gtccgctatc ccgcggatgt cttcctcttt gccgcgaaaa gcaaaagccg
     1381 acgtacgcgc aatgaccttg agccctgaaa tctgcgccag caggttgatg atttcttcgg
     1441 ccaggccgtc ggagaagtat tcctgatctt tctccgcgct gaggtttgca aagggcagca
     1501 ccgctaggga gggagtggct gcggtaaggt tggcgccact ctgagatggg tgcctcgacg
     1561 gcgccggctt ctgcccgtcg gcaatctcca gacccgcctt tcgaaggcct tcgatcatcc
     1621 cctcgacaag gtctggtccc cagaaaatcg cgaggtcctt ccgcgcctct gctttgaagt
     1681 tcggccgccg ggtcaacaga gtctgcagca gaatgcgtgc cgattcgatc tcgccaagct
     1741 gcccgagggc tgccgccatg attgcgcttg cgcgccagag gctgggcatg ttcactttgc
     1801 gggcaaactc aagagagagg cggtaatcgc ctttgcgata ggcgtcgaag cacggaacaa
     1861 accaatacca tcccgggtag tgcggattga gactgcgagc cttggccgac aacctgcaac
     1921 catattccca gtcgccgtcg tacgcgataa aggaacccag gaaagcgatg gagaatccgt
     1981 ccatggggtt gagctccagc gcacgctccg ccgcgctccg gaaggcaggc cgatccccaa
     2041 ggaaataatt cgccatcgca agagcgtgaa acgcgaggtg attggacggc gcggcttcaa
     2101 ccgcgcgcaa cgcagctgcc atcgcccggc ccagcggatc gggccggaga ttgagttcgt
     2161 gggtgaattc ttccttgcag atgagcgaca gcatagccca ggcatcgccg tagcctggct
     2221 gcacttccac cgcgcgttcc aaggcggcac gcgacgcggc atgctcctca gcgttgacac
     2281 gctggaagtg cgcgaagctg cgcagcaccg cctcgtacgg gctgagatcg gatgaattcc
     2341 tggtccgaag tatgtccgac atggtgcggg gcagaatgcc ttgtgtgtcg gcaacagtgg
     2401 aaacgatgcg aggaacgaga tcgtcctgca actcgaagaa agcatccggg ctgaaagcgc
     2461 gctcgtaatt ctcggcccaa agatgggcgc ccgtgatgcc atccacaagc tggacagata
     2521 ggcgcagctt cgcacctgcc tggcgcaggc tgccttccat cacgtagcgc gcgccgagct
     2581 cgtggccggc ggtgcgaaca tcgactccgc cttgtgagta ccgtgacgtc gagctgcgtg
     2641 caatgacgcg caggtaggag aagcgcgaaa gcccggtgac aatctcttca cccagcccat
     2701 cggccagcgt cgcgacctct gggcttgcgc cgatcgactt gaaggggagc acggcgatcc
     2761 agaaaccttc atcagtatgg gctg
//
LOCUS       DATMGX010000064         2391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_18833, whole genome shotgun sequence.
ACCESSION   DATMGX010000064 DATMGX010000000
VERSION     DATMGX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18833"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VNR65_01030"
     CDS             complement(<1..273)
                     /locus_tag="VNR65_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17287.1"
                     /translation="MRAHDFFYGRMKMGMRAILFGVGVSLVGVSAANAADFTSRGSLK
                     DAPFVEVQCIWCGFYGGFSVGYGSGVAKNYVSPNANAPHGWANNDPS"
     gene            complement(597..1313)
                     /locus_tag="VNR65_01035"
     CDS             complement(597..1313)
                     /locus_tag="VNR65_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HWJ17288.1"
                     /translation="MTASHPQNRKRFYAAATVAPCENGSFGIELDGRPIKTPAGHALA
                     VPTQSLAEAIAGEWNAQGNDIVPASLPLTRLANSAIDAVAEREGDVVQDILNYTASDL
                     LCYRAASPAGLVANQARLWDPILAWARIEYDAPFQVGTGVTHLEQPTESLDAVRQVIS
                     AFGPFQLAALHVITTLTGSALIALAHAQRFLDTDAAWEAANVDENWQVSQWGEDFEAA
                     QRQKKRFSEFLNASRFFDLV"
     gene            complement(1323..2189)
                     /locus_tag="VNR65_01040"
     CDS             complement(1323..2189)
                     /locus_tag="VNR65_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HWJ17289.1"
                     /translation="MTSDDTKQRRRDEEAAAALVRQRDRDRYWSALFAPRVKRPAVFA
                     LYAFNAELDHILASVSEPMAGQIRLQWWRDTVDFATPGTRTGNPVADALSAAITEHNL
                     SKDRLLGMVDARVPAMFGEPPLDDKALKTSLRETEGAVFELVAAILGDRSDSAKAAAA
                     HAAVAYGLTETLLKLPFLASRQKAVLPSSYIESRGIDLAAIHRGETSESFGAAVADLR
                     GVASRALQQFRAVAGDLDRSAWPAFLPLTLVKPYLRAMAAPDFDPLQTVVAISPLRRF
                     WRIWRAARRHTL"
BASE COUNT          532 a          667 c          698 g          494 t
ORIGIN      
        1 gctcggatcg ttattcgccc atccatgagg tgcattggcg ttgggggaca catagttctt
       61 tgcaaccccg ctgccgtaac cgaccgagaa accgccgtag aagccgcacc agatgcattg
      121 cacctcgaca aaaggcgcgt ccttcaaact accgcgactt gtgaaatcgg cagcatttgc
      181 agcgctcact ccaaccaagg atacgccgac gccaaaaaga atagccctca tccccatttt
      241 cattctcccg tagaagaagt catgcgcccg caccacatgt cttctgtttt gccggatatc
      301 tcgagataaa ctcgatgggg tagatctata ctgataggaa tggttatata tcggcgagaa
      361 agcggcgttt tggcctttta taagaccgcg ccgtgcctca acctcaggtt taacctattg
      421 tttctaagat catttcctga taggaacggc cttggcatgc cacaagaagc cacatttcac
      481 tgaataaatc ttagaattgg tttacaatcc tttagactaa tacaacggat aacatgcgac
      541 aaaatatttt atactgcgac gggaataaat attttttgct gcaatgggaa tagaattcag
      601 accaggtcaa aaaaccgcga agcattcaaa aattcggaaa agcgcttttt ctgtctttga
      661 gcggcttcga agtcttcgcc ccattgagag acctgccaat tctcgtcgac attggccgcc
      721 tcccaggcag cgtccgtgtc gagaaaccgc tgagcatgcg ctagcgcgat gagcgcagaa
      781 cctgtcagcg tggtgatgac gtgcagcgca gccagttgga atggtccgaa cgccgagatc
      841 acctggcgga cggcatcaag ggactctgtg ggctgctcca gatgcgtcac gcccgttcca
      901 acctggaagg gcgcgtcata ctcgatgcgt gcccaggcaa ggatcgggtc ccagagacgc
      961 gcctggttcg ccacaagccc ggccggagac gcggcgcgat agcaaagaag gtcagacgcc
     1021 gtgtagttca ggatgtcctg caccacatcg ccttctcttt ccgcaacggc atcaatggcg
     1081 ctgttcgcaa ggcgggtgag cggaaggctg gcgggtacaa tatcgttccc ttgcgcgttc
     1141 cactcgccag caatcgcctc ggcaagggac tgcgtgggca cagcgagcgc atgaccggct
     1201 ggggttttga tcggccgtcc atcgagttcg atgccgaaac ttccattctc acagggtgca
     1261 accgttgcgg cggcgtagaa gcgtttcctg ttctgaggat gcgatgcggt catggtttgc
     1321 tttcagagcg tgtgccgccg cgctgcccgc cagatccgcc agaaccggcg taaggggctg
     1381 atcgcaacaa cggtttggag tggatcgaaa tccggcgcgg ccattgccct tagatagggt
     1441 ttgacgagag tcagtggaag gaaggcaggc caagccgagc gatcgagatc acccgccacc
     1501 gcacggaatt gctgcagcgc ccggctggcg acaccccgaa ggtcggcgac ggcagccccg
     1561 aagctttcgc ttgtctcgcc cctgtggatg gcagccaaat caattccgcg gctctcgatg
     1621 tacgacgatg gcaagaccgc cttttggcgc gaggccagga aaggaagctt gagaagcgtt
     1681 tcagtcagtc cataggcaac ggcggcgtgc gcggcggctg ctttcgcgga gtcgcttctg
     1741 tcaccgagga tggcagccac caactcgaag accgcacctt cggtctcgcg cagagaggtc
     1801 ttcagggcct tgtcgtccag gggaggttcc ccgaacatgg caggcacacg ggcgtcgacc
     1861 atgcctagga ggcgatcctt ggagaggttg tgctcggtga tcgcggcaga aagagcgtcg
     1921 gctacggggt ttccggttct tgtccccggc gttgcaaagt ccaccgtgtc gcgccaccat
     1981 tgcaggcgga tttggccagc catgggttcc gagacggacg ccagaatgtg gtccaactcg
     2041 gcattgaagg cgtagagcgc gaaaacggcc ggacgtttga ccctgggcgc aaaaagggcg
     2101 ctccaatagc ggtcgcggtc gcgctgcctg acaagggctg ctgcggcctc ctcgtcacgt
     2161 cttctttgtt ttgtgtcgtc cgaggtcaat gagggcgctc caagcaacca ttcaactaaa
     2221 agccttagat caacccgcct ttaatcggaa acgattagag gcgcaaaagt tgatcgattc
     2281 taaaatgctc tagaaggtgg ccgggatatt cgcaagggga agaagcgccg ccgtgaacat
     2341 gcgcgcttct gcaacaagga cattgtaggt ccggcagtcg gcactggtgt c
//
LOCUS       DATMGX010000065         2146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_400247, whole genome shotgun sequence.
ACCESSION   DATMGX010000065 DATMGX010000000
VERSION     DATMGX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400247"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2013)
                     /locus_tag="VNR65_01045"
     CDS             complement(<1..2013)
                     /locus_tag="VNR65_01045"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HWJ17290.1"
                     /translation="MKTNTLTPDSLQKTNAYWRAANYLSVGQIYLYDNPLLKQPLKLS
                     DVKPLVVGHWGTTPGQNFIYVHLNRVIKKYDLDMFYIAGPGHGGPAIVGNVYLEGTWS
                     EVYPNVTQDEAGLKKLFMQFSFPGGISSHVAPTTPGSIHEGGELGYSLSHAFGAAFDN
                     PGLIVACVVGDGEAETGPLATAWQSNKFLDPITDGAVLPILHLNGYKISNPTVLARIE
                     HEELEQFFRGCGWEPYFVEGDEPETIHQLMATTLDKAIEDIQQIQKNARDKGDTTRPL
                     WPMIVLRTPKGWTGPKVVDGLQIEGTFRSHQVPILVDSEHPDHVELLEDWMNSYRAEE
                     LFDDNGRLRPELAELAPKGDRRMGANPHTNGGILLRDLRMPDFHDHAVNVPAPGAVDG
                     QDTLVLGKFLRDVAKLNQDQRNFRVFGPDETLSNLLGAVFEVTNRQWEAREAKNDEFL
                     APAGRVLDSMLSEHQCEGWLEGYLLTGRHGLFNSYEAFIRIVDSMFSQHAKWLKVTLE
                     LPWRRKIASLNYLLASHVWQQDHNGFTHQDPGFLDHVINKKADIVRVYLPPDANCLLS
                     VFDHCLRSRHYVNVVVAGKHALPQWLTMDAAVVHCTEGIGIWQWASNDQDAEPDVVMA
                     CCGDTPTLEILAAVSILRQHLPDLKIRVINVVDLMKLQSASEHPHGL"
BASE COUNT          434 a          630 c          608 g          474 t
ORIGIN      
        1 tagcccatgg ggatgctcac tggcagactg cagcttcatc agatcgacga cgttaatgac
       61 ccggattttg agatcaggca gatgctggcg cagaatggaa actgccgcca gaatctccag
      121 cgtgggcgtg tcgccgcaac aggccatcac cacgtccggc tcggcgtcct ggtcattgct
      181 ggcccattgc cagatgccga ttccctccgt gcaatgcacg acagcggcat ccatggtcag
      241 ccattgcggg agggcgtgtt tgccggccac caccacgttg acgtaatgcc ggctgcgcag
      301 gcagtgatca aagaccgaca gcaggcagtt ggcgtcgggc ggcaggtaaa cgcgcacgat
      361 atcggccttc ttgttgatga cgtggtcgag gaacccggga tcctggtgcg tgaagccgtt
      421 gtgatcttgc tgccagacgt gggaggcgag cagatagttc agcgacgcga tcttcctccg
      481 ccagggcagt tccagcgtta ccttcagcca cttggcgtgc tggctgaaca tcgaatcgac
      541 gatgcgaatg aaggcctcat agctgttgaa cagcccgtgc cgtccagtca gcaaatatcc
      601 ttccaaccaa ccctcgcact ggtgttcgct gagcatcgag tccagcacgc gtccggccgg
      661 cgcgaggaat tcatcgttct ttgcttcacg agcctcccac tgacggttgg tgacttcgaa
      721 aaccgcgccc aggagattcg agagtgtctc atccggaccg aacacacgga agtttcgctg
      781 atcttgattt aacttggcca cgtcgcgcag gaacttacca agcacgagcg tatcctgccc
      841 gtccaccgct ccgggcgcag gcacattcac cgcgtggtcg tggaaatccg gcatgcgtag
      901 gtcgcggagc agaatgccgc cgttggtgtg cggattggcg cccatccgcc ggtcgccctt
      961 tggggccagt tcggccagtt ccggtcggag acgcccattg tcatcgaaca actcctcggc
     1021 cctatagctg ttcatccagt cttcgagcaa ctcgacatgg tcaggatgct cggaatccac
     1081 aaggatcggc acttggtgtg agcggaaggt accctcgatc tgtagtccgt cgaccacttt
     1141 cggtcccgtc cagcccttgg gtgtcctcag gacgatcatt ggccagaggg gccgagtcgt
     1201 atcgcctttg tcccgcgcat ttttctgaat ctgctggatg tcctcaatgg ccttatctag
     1261 ggtagtggcc ataagttggt gtatcgtctc cggctcatct ccctccacga agtagggctc
     1321 ccagccgcag cctctgaaga attgttctaa ttcctcgtgc tcgatccgcg ccaggacggt
     1381 cgggttgctg atcttgtagc cattgaggtg caggatcggc agcacggctc cgtcggtaat
     1441 cgggtcaaga aacttgttgg actgccacgc agtcgccaag ggccccgttt ctgcctcgcc
     1501 atcaccgacc acgcaggcga cgatcagacc gggattgtcg aacgccgctc cgaaagcatg
     1561 gctgagcgag taccccaact caccgccttc gtgaatcgac ccaggcgtcg tcggcgcaac
     1621 atggctcgaa atcccgcctg ggaacgagaa ctgcatgaac agtttcttga ggcccgcctc
     1681 gtcctgcgtg acgttcggat aaacttcact ccaagtgcct tcgaggtaaa cgttgcccac
     1741 aatcgcgggg ccgccgtgcc cgggtccggc gatgtagaac atgtccaggt catacttttt
     1801 gataacccgg tttaaatgca cgtagatgaa gttctggccg ggagtggtgc cccaatgccc
     1861 gacgaccagg ggcttgacgt ccgacagttt cagtggctgc ttcaacagcg gattgtcgta
     1921 caggtagatc tggccgaccg agagataatt ggcggcccgc cagtaggcat tggtcttttg
     1981 cagtgaatcc ggggtgagag tattcgtttt catattttaa aatagattaa agcttcccct
     2041 tcgcacgaaa agtgaagctc acattttact acgcagtcgg catcatgaat ttatcgaccg
     2101 cacgattaac tgtttaccag ttcgtcaacc caataacctt tgcgat
//
LOCUS       DATMGX010000066         3562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_18620, whole genome shotgun sequence.
ACCESSION   DATMGX010000066 DATMGX010000000
VERSION     DATMGX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18620"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /gene="mnmE"
                     /locus_tag="VNR65_01050"
     CDS             complement(<1..939)
                     /gene="mnmE"
                     /locus_tag="VNR65_01050"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HWJ17291.1"
                     /translation="MTIYALSTAPGRAGIAVIRISGRAARNALDALCHGRFPASRVAS
                     FRRLRHPHTGEVLDEAVVLWLPGPASFTGEDVAEFHVHGGRAVVAGVLEALASFGLRL
                     AEPGEFTMRAFGNGKIDLTEAEGLADLINAETAYQRRQAIAQHCGALRGHYDHWRTTL
                     LSAMAYVEASLDFSDEGDVGLNPFQEAVPIVRGLVGELERALADGHRGEIVREGIQVA
                     IVGAPNVGKSSLLNALAGREAAIVFHEPGTTRDVIEVALDLDGFPFVLRDTAGLRETE
                     SPVEQEGIRRALASAKDADVVLLMYDATTPHPMGEGT"
     gene            1077..3134
                     /locus_tag="VNR65_01055"
     CDS             1077..3134
                     /locus_tag="VNR65_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWJ17292.1"
                     /translation="MALNRLSEETSPYLQQHKHNPVHWWPWCQEAFEEAQRLDKPVLL
                     SVGYAACHWCHVMAHESFEKADVAELMNELFVNIKVDREERPDVDTLYMTALQELGEQ
                     GGWPLTMFLTPDGMPFFGGTYFPDTPRYGRPSFKDVLVNVARVFATEKETIAQNTAYL
                     KSRLMPRLNYGAAPEFSEEQLAAIASKFIGAIDPTNGGLRGAPKFPNTSIFTFLWRAG
                     LRYGLKTCLEEVKNTLLHICQGGIYDHIGGGFSRYSVDERWLVPHFEKMLYDNALLIE
                     LMTEVWKETQSDRLRSRVSETIAWLSRTMLAPDGGFASSYDADSEGAEGRFYLWTAKE
                     ITNVLGHDEEAALFAQVYDVTEGGNWEGGTILNRLNALALLSHHEEQILDECRHKLFQ
                     ERNKRKKPSWDDKVLADWNGLAIRALARAGDTFATPEWIKLAEGAYAFVCSRMISGGR
                     LYHSYRGNKLKGPATSTDYANMISAALALYQVTNEKRYLEDAVAWTAIMNRHYDAESG
                     GYYLTADDTSDLILRPLSASDDAVPNPNATMLQNLADLYTFTGDAAYLKRADALLDAF
                     QGAAQTMALAYTGLLSGALTLISPQHIVIAGNRADPDAAAWRRALTEVSMPGAIIQWV
                     DDGLSIPPSSPAAGKGKVDGKITAYICIGQRCSLPLIDPDLVKEKLKEDRHVSVQVAA
                     API"
     gene            3330..>3562
                     /locus_tag="VNR65_01060"
     CDS             3330..>3562
                     /locus_tag="VNR65_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWJ17293.1"
                     /translation="MSEPVFEALCVAFDQLSVAVLILSASGRILFANQAASAMLEEGW
                     PIRLLDGCLQGKDRGVSAALKQTIEFVSLNQQG"
BASE COUNT          755 a         1093 c         1035 g          679 t
ORIGIN      
        1 tgttccctct cccatagggt gaggggtggt cgcgtcatac atgagaagaa ccacatccgc
       61 gtccttggcg ctcgccagcg cccggcgaat gccctcctgc tctacaggac tttcggtctc
      121 gcgaagccca gccgtatcgc gcaacacgaa ggggaagccg tcgaggtcga gagccacttc
      181 gatgacatca cgcgtcgttc ccggctcatg gaagacaatc gccgcttcgc ggcccgccaa
      241 cgcattcagc aggctcgact tgccgacatt gggcgcaccg acgatggcca cttgaatgcc
      301 ttcgcgaacg atctcgcccc gatggccatc ggccaaagcg cgctccagct cgccgacgag
      361 gccgcggaca attggcactg cctcctggaa tgggttcaac ccaacatcgc cctcatccga
      421 aaaatcgagg ctcgcctcca cataagccat cgcactgagc agcgtggtgc gccaatggtc
      481 atagtgaccc cgcaaagcac cgcagtgttg cgcaatcgcc tgccgccgct gataagctgt
      541 ctcggcgttg atcagatcgg caaggccttc ggcttcggtt agatcgatct tgccgtttcc
      601 aaaggctcgc atcgtaaact ctccgggctc cgcaagcctc agcccgaacg atgcgagcgc
      661 ctccagaaca ccggccacga ccgcgcggcc gccatgaaca tggaactcgg cgacatcctc
      721 gcccgtgaag cttgccgggc ctggaagcca gagcacgaca gcctcatcca gaacctcgcc
      781 ggtatgagga tgacgcaatc gcctgaagct cgcgacccgc gacgctggga aacggccgtg
      841 acacagcgca tcaagcgcgt ttctcgccgc tctgcccgaa atccgtatga cggcaatacc
      901 cgcacgccct ggagcggtgg aaagggcgta aatggtcatg gcggctcgtt cctggcggca
      961 aatgcttgca tgacatattg caggatgcat acgcgaaaaa agcgtatatt catattgacg
     1021 ggttgtcgtg tttctggcca ctcccaaaag agcaaagcgg tgaacaaggt tgcctcatgg
     1081 cattaaatcg tctgagcgaa gaaactagcc cctacctcca gcagcataaa cataatccgg
     1141 ttcattggtg gccttggtgc caggaggcct tcgaggaggc gcaacggctc gacaaacctg
     1201 tgctgttatc ggtcggctat gccgcctgcc attggtgcca tgtgatggcg catgagagct
     1261 tcgagaaggc tgacgtggcc gagctcatga acgagctgtt cgtgaacatc aaggtcgaca
     1321 gggaggagcg ccccgatgtc gacacgcttt acatgacggc cttgcaggag ctcggcgagc
     1381 agggcggctg gccgttgacg atgttcctca cccctgacgg gatgcccttc ttcggcggca
     1441 cctactttcc cgacacgccc cgttacggcc ggcccagctt caaggacgtg ctcgtgaacg
     1501 tggcccgcgt cttcgcgacg gaaaaggaga ccatcgcgca gaacaccgcc tatctgaagt
     1561 cacggctcat gccccggctc aactatggcg ccgcgcccga attctcagag gaacagcttg
     1621 cggccattgc cagcaagttt atcggcgcga tcgatccaac caatggcggc cttcgcgggg
     1681 cacccaaatt tccgaacacg agcattttta cgttcctttg gagggccggc ctgcgctacg
     1741 ggctgaagac ctgcctggaa gaagtgaaaa acacgcttct ccatatctgc cagggaggca
     1801 tctacgatca catcggggga ggcttctcgc ggtattcggt cgacgagcgc tggctcgtgc
     1861 cccacttcga gaaaatgctg tacgacaacg cgctgctgat tgagctgatg acggaagtct
     1921 ggaaggagac ccaatccgac cgcctgcgca gccgcgtgag cgagacaatc gcgtggctgt
     1981 cacgcaccat gctcgcgccc gatggcggct ttgcttcgtc ctacgatgcc gattccgaag
     2041 gggcggaggg aagattttac ctctggaccg ccaaggagat cacgaatgtt ctcggccacg
     2101 acgaagaagc ggcactcttc gcacaagtct acgacgtaac cgaaggtggc aactgggaag
     2161 gcgggaccat cctcaaccgc cttaacgcgc tggcccttct gagccaccac gaggagcaga
     2221 tcctggacga atgccgccac aagcttttcc aagagcgcaa taagcggaag aagccgagct
     2281 gggacgacaa ggtactcgcc gactggaacg gtctagccat cagggcgctg gcgcgtgcgg
     2341 gcgatacctt tgcgacgccg gaatggatca agctcgcgga aggcgcttat gcctttgtct
     2401 gcagccgcat gatctccggc ggccgtcttt accattccta tcgcgggaac aagctcaaag
     2461 gccccgcgac ctcaactgac tacgcgaaca tgatttcggc ggccctggcg ctttaccagg
     2521 tgacgaacga gaagcgctat ctcgaggacg ccgttgcctg gaccgcaatc atgaaccggc
     2581 attatgacgc tgagagcggc ggctattacc tcaccgccga cgacaccagc gaccttattc
     2641 tgcggcccct ttccgcgagc gatgatgcgg tccccaatcc gaacgcgacc atgctccaga
     2701 atttggcgga cctttacacc ttcacgggcg acgccgccta tctgaagcgc gcagacgcgc
     2761 ttttggacgc cttccagggg gcggctcaga caatggcgct ggcctatacc ggtcttttgt
     2821 ctggcgcgct cacgctgatt tcgccgcagc atatcgtcat cgcgggtaac cgggcggacc
     2881 cggatgccgc cgcctggcgc agggcactga ctgaggtatc catgcccggc gcgatcattc
     2941 agtgggtgga cgatgggctc agcatcccgc cgtcatcgcc tgcggcaggg aaaggtaagg
     3001 tcgacggaaa gataaccgcc tacatttgca tcgggcagcg ctgctccctg ccgctgatcg
     3061 acccggacct ggtaaaggaa aaactcaagg aagatcgtca cgtttcggtg caggttgcag
     3121 cagctccgat ctagagtctg ctaagatgcc aatcagcgtc aattgatgca ttcgcataat
     3181 tagtttatta tatcctgaat ccacctggaa atattgccgg gatgccgtgc gctttcccaa
     3241 cgacaacctt tccacgcatc cccaggttgg ttatttgaat agatgaaatg ttggcgccag
     3301 cagggagccg gccgcccaga ggcgttctaa tgtccgagcc tgtttttgag gccttgtgcg
     3361 ttgcgttcga tcagctgagc gtcgcggttc taatcctttc agcgagcgga cggattctct
     3421 tcgcgaatca agccgccagc gcgatgctcg aagagggttg gcccattcgc ctgttggacg
     3481 ggtgcctgca aggaaaagac cgcggtgtga gcgctgcgct gaaacagacg atcgaattcg
     3541 ttagcttgaa ccagcaaggc ga
//
LOCUS       DATMGX010000067         5554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_155303, whole genome shotgun sequence.
ACCESSION   DATMGX010000067 DATMGX010000000
VERSION     DATMGX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155303"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..938
                     /locus_tag="VNR65_01065"
     CDS             78..938
                     /locus_tag="VNR65_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWJ17294.1"
                     /translation="MSLTKFVPTPKFEEYKERFKEHYKLERRADGVILAQGHTLGGPM
                     QLSVENHRSLGQLLKTIGADPENEIMILTGTGKDFMMDADPEGFALEQEDLRHWAYEY
                     AYKDGRINVSSLVNDLEIPTIGVLNGPGFHTEICLMCDITICAEDAIIYDLHYDIGSV
                     PGDGIHSCFQELLGVKRAAYALLTGQAIDAKTALEYGMVNEVVPRDKLLDRAWQIADH
                     IMTQPRITRRLTTQIVRRPWRQRITNDLDGGFGIQMFAHLAKDKAVHGRNHIGSKVAY
                     VRKGKKNNFD"
     gene            1090..2355
                     /locus_tag="VNR65_01070"
     CDS             1090..2355
                     /locus_tag="VNR65_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWJ17295.1"
                     /translation="MSSITRRKFTKVALAASATMAAPAFMASGAEKPIRIGFSIAQTG
                     GIAAGGKAGLTALELWRSDVNANGGLLGRPVEFVVYDDQSQTSTVPGIYAKLLDIDKV
                     DLLLSPYGTNLTAVVMPMIKQRNRFVIAQFEIGQNDELKHDKFFEIAPWGPKPGANWC
                     RGYFDLAKRQGYKTIGIISSDAEFSANAANIGVKVAGEYGMKVISNQHYPPNSVDFSG
                     ILRNVNAAAPDFIFVASYPVESAALVHGISEIGIADSVQMFGGAIVGIQYAQQLESLG
                     SALNGVTNYDSYVVAPTMNFAGIDKFLERYRPIAESKGVDALGHFLPPFFYAGGQLIE
                     KAVKAVGSLDESKLAQWLHNNAIDTIVGPIKFGPDGNWVENRIVMAQYRGVKDKDLDQ
                     FRHPGKQIILQPAALATGTIVAPYKKARA"
     gene            2427..3323
                     /locus_tag="VNR65_01075"
     CDS             2427..3323
                     /locus_tag="VNR65_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWJ17296.1"
                     /translation="MIKVPSLMIVELGANAVVTGLLLGGLYAVASLGLTIAFGLLQIP
                     NVAHPTFMVLGAYCAYFGNLLGVDPVIASIIFAPVFYLVGMIFYQAYFVCFERRGRGN
                     TLQGFTFFFGVAMVLEVALILAVGVDMRSVDAAYIGKSLTIGIITMPYRFIIPFIIGS
                     VVGAAMWLFLTRTHMGLAIRAVAHDDNALRIVALNPAQVKRHAFGIAMMTAALAGAAL
                     IVVNPVDPFIGREYIGRSFAIVVLAGMGSVPGTLVAAVIIGLLESLVITFVGATWAPG
                     VAFAVLLATLAIRPSGIFGVSR"
     gene            3374..4267
                     /locus_tag="VNR65_01080"
     CDS             3374..4267
                     /locus_tag="VNR65_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWJ17297.1"
                     /translation="MLTHLVTNAYVFYAGFVALQYVVIATGWNILGGYAGYVNFGSGA
                     FVGLGMYAAVFLFKAIGAPLWVQVLGAAFAGGVLGLGMGYLTLRIQGVFFSIATLALT
                     VVLNTIIVNWSFVGGARGATVLAPPPPAWFPSIISYVFFAMLVLAVVSVAVARWIEKS
                     WIGRGLAALRADEQAAECSGTPTLKLKLFAAAVSGALIATAGAPYPYYASYVEPASAF
                     GLAIALNALAMPLIGGTRSWIGPLIGALLLSGLEQAATVTIPTEVNLLIVGLVLIGFV
                     VAAPDGLLGLFDRKSTHGRRH"
     gene            4264..4977
                     /locus_tag="VNR65_01085"
     CDS             4264..4977
                     /locus_tag="VNR65_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ17298.1"
                     /translation="MNEPILSVRGVSKRFDTLIAVADVDLDVARGEKLGIIGPNGSGK
                     TTLINCITGVIHPEEGKITFEGIDISNMPTYRRARRGIARSFQIPRPFVGMTVIQNLL
                     VPLDYVESGKRDPAGAREILSTVGLKARAEDPAESLTHLELRKLELARALAAEPRLLI
                     ADEVMAGLSEPEIDEVLEILFTLNSRGVTVIMIEHIMHAIMRFSKRLVCMETGRIIAD
                     GTPQEVGRHPEVRRVYFGK"
     gene            4967..>5554
                     /locus_tag="VNR65_01090"
     CDS             4967..>5554
                     /locus_tag="VNR65_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ17299.1"
                     /translation="MVNRLVVENLSAGYGAVSVLRGVSLEIREGELVALLGTNGNGKS
                     TLLNAILGLVRPTGGSVRLEWDGEITELAGSPAEKIVERGIALVPEGRRLFPHLTVDE
                     NLTLGGCGRRARHNLRHNLEFVFAAFPVLQERRRQPAGTMSGGQQQLLAIARALMTEP
                     RIVLIDEPSVGLAPIAVDQVLSTVRELQANRGLTVL"
BASE COUNT         1151 a         1490 c         1623 g         1290 t
ORIGIN      
        1 ataaatgaaa cggcgcgtgg caggaaaaac gagaaagaag acgaaccaga tcacacgagg
       61 tgcatggaga accagctatg tctttgacaa aatttgttcc gacaccgaag tttgaggaat
      121 acaaggagcg attcaaggaa cattacaagt tagaacgccg cgcggatggc gtgatcttgg
      181 ctcaggggca cacactaggc gggccaatgc agttgagcgt cgaaaaccat cgctcgctcg
      241 gacaacttct taagaccatt ggagccgacc ccgaaaacga aatcatgatt ttaacgggga
      301 cgggcaaaga ctttatgatg gacgcagacc cggaaggctt cgcacttgaa caggaagatc
      361 tgcggcattg ggcctacgag tacgcctata aggacggcag aattaacgtg agctcactcg
      421 tcaacgatct cgaaattccg acaatcggtg ttctgaacgg tccgggattt cacaccgaaa
      481 tttgcctcat gtgcgacatc acgatctgtg ctgaggacgc gataatctac gatcttcact
      541 atgacatcgg gtcggtgccc ggagacggga ttcacagttg ttttcaggaa ctactgggtg
      601 tgaagcgagc ggcctatgca ttgctcacag gccaggccat cgacgccaaa accgcgctgg
      661 aatacggaat ggtcaatgag gtggtgcctc gcgacaagct actagaccgc gcgtggcaga
      721 ttgccgatca catcatgacg cagcctcgca ttacccgtcg ccttacgacg cagatcgtac
      781 gccgcccttg gcggcagcgg atcacaaacg acctggatgg cggctttggc attcagatgt
      841 tcgcgcatct cgccaaagat aaggccgttc acggcaggaa tcacatcggt tcaaaagttg
      901 cgtacgtcag gaaaggaaag aaaaataact tcgattagcg tggcatacat ccgtgggcgg
      961 tgaggtttgg agatagcgaa aagctcgtca attgcgagcg ctagcagcgg ggcgcgtctg
     1021 gagacatcgc cgcacgcgat ccgcggagac ggaatttgta attggattca actcaacggg
     1081 agtacaacga tgagcagcat caccaggagg aaattcacca aagtagccct cgctgcatcc
     1141 gcaacgatgg ctgcaccggc tttcatggca agcggtgcgg aaaaaccaat ccgaatcggg
     1201 ttcagcatcg cccagacagg cggcatcgcc gcgggaggaa aagccgggct gacggcgctt
     1261 gaactatggc gcagcgatgt gaatgcgaat ggcggcctac tcggccggcc ggtagaattc
     1321 gtcgtctacg atgatcagag ccaaacaagt actgtgcccg gcatctacgc caagctgctc
     1381 gacatcgata aggtcgatct tttgctttcg ccttacggca caaacctgac tgctgtggtc
     1441 atgcccatga tcaaacagcg caaccggttt gttatcgctc agtttgaaat cggccagaac
     1501 gacgagctca agcatgacaa attcttcgag atcgctccct ggggtcccaa gccgggcgcc
     1561 aattggtgcc gtggctactt cgaccttgcc aagcgtcaag gctacaaaac gattgggatc
     1621 atcagttcag atgcggaatt ctcggcaaat gccgccaaca taggcgtcaa ggtcgccgga
     1681 gagtacggca tgaaagtgat atcgaaccag cattatccgc caaactcagt cgacttttcc
     1741 ggaattctgc gcaacgtgaa tgcagcggca cctgatttta tcttcgtcgc ttcctatccg
     1801 gtcgagtctg ctgctttggt ccatggcatt tcagaaatcg gtatcgccga cagcgtccag
     1861 atgttcggcg gcgctatcgt cggaatccag tacgctcaac agctcgaaag cctcgggtct
     1921 gcgcttaacg gcgtcaccaa ctatgacagt tatgttgtcg cgccgactat gaacttcgcc
     1981 ggcatcgaca aattcctcga gcgttatcgg ccgatcgccg agagcaaggg ggtcgatgcc
     2041 ctcgggcact tcctgccgcc gttcttttac gcgggtggcc aactcatcga gaaggccgtc
     2101 aaggcggtcg gctcgcttga cgagtcaaag ctcgcgcaat ggctgcacaa caacgcgatt
     2161 gatacgatcg ttgggccgat caagtttgga cccgatggca attgggtgga aaaccggatt
     2221 gtcatggctc agtaccgagg cgttaaggac aaggacctcg atcagttccg tcatcccgga
     2281 aaacagatca tcctgcagcc cgcagcctta gcgaccggga cgatcgtcgc gccctataag
     2341 aaagctcgtg cctgataggc gcgtgttgat cgcttaatgg agttggcgcc ggcctaggct
     2401 aaaactatcc tgggccggcg ccgtatatga tcaaggtgcc gtcgctgatg attgttgaat
     2461 tgggtgcaaa tgccgttgtc accggcttgc tgctcggcgg tctttacgcg gtggcgagcc
     2521 tcggtctcac tattgccttc gggctactgc aaatccccaa tgttgcgcac ccgaccttca
     2581 tggtgctggg cgcctactgc gcatatttcg gcaacctgct gggggtagat ccggtcatcg
     2641 cgagtataat atttgcgccg gtcttttatc ttgttggcat gattttctat caagcttatt
     2701 tcgtgtgctt cgagcgtcgc ggccgcggca atacattgca aggcttcacc ttcttctttg
     2761 gcgttgccat ggtgcttgag gttgcgctca tacttgccgt cggcgtcgac atgaggtcgg
     2821 tcgacgcagc ctatattggt aaaagcttaa cgatcggcat catcaccatg ccgtatcgtt
     2881 tcatcattcc cttcataatc ggatcggtcg ttggagccgc gatgtggcta tttctcactc
     2941 gcactcatat gggcttggcg atccgcgcgg tcgctcatga tgacaacgct cttagaatcg
     3001 tcgcgctcaa tcctgcccag gtcaagcgac acgcctttgg catcgcgatg atgaccgccg
     3061 ccctggctgg ggccgcgcta atcgtcgtaa acccggtgga tccctttatc ggccgcgaat
     3121 atatcggccg atcatttgct atcgtcgtgt tggccggtat gggaagcgtg ccaggaacgc
     3181 tcgtcgctgc ggtcatcatc ggtctgttag aaagtctcgt gattacgttc gtcggcgcga
     3241 cctgggctcc tggtgtcgct tttgcagttc tgctcgcaac cctcgcgatc aggccaagtg
     3301 gcatattcgg tgtgtcacga tgagcggatg gcgattcaac gtcggtatag ttgtggttct
     3361 tgccggcggt gcgatgctga cgcatttggt gactaacgct tatgtcttct acgcgggctt
     3421 cgtcgcactg caatatgtgg tcatcgcgac tgggtggaat attctgggtg gatatgcggg
     3481 ctatgtgaat ttcggcagcg gtgcgttcgt tggcctcggc atgtatgccg ccgtcttcct
     3541 gttcaaggcg attggggccc cgctgtgggt ccaggtattg ggcgctgcct ttgctggcgg
     3601 cgttttgggc cttgggatgg gatacctgac tttgcgcatt cagggggtat tcttttcaat
     3661 cgccactctt gccctgaccg ttgtactcaa cacaatcatc gtcaattggt cctttgtcgg
     3721 cggtgcgcgc ggagcgacgg tcttggcacc gccgcctccg gcatggtttc cgagcattat
     3781 cagttacgtc tttttcgcca tgctggtcct tgcggtagtc agtgttgctg tggcgcgctg
     3841 gattgaaaaa tcatggattg ggcgcggcct cgccgcattg cgcgccgacg aacaggccgc
     3901 cgagtgcagc ggtacgccga ctctgaagct caaattattt gctgcagccg tcagcggtgc
     3961 tttgattgca accgctggcg ctccttatcc ttactatgct tcatacgttg agcctgcttc
     4021 ggccttcgga ctcgccattg ctcttaatgc cctcgcgatg ccgttgattg gcggtacgcg
     4081 cagttggatt ggtccgctga tcggtgcgct tcttctcagc ggtttggagc aagcagcaac
     4141 tgtgaccatt ccgactgagg tcaatctatt gattgtaggg ctcgtgctta tcggttttgt
     4201 cgtcgccgcg ccggatggat tgctgggctt gttcgatcgc aagtctaccc acgggaggcg
     4261 gcattgaacg agccgatctt gtccgtacgg ggcgtatcga agcgtttcga tacccttatc
     4321 gctgttgcgg atgtcgactt ggacgttgcg cgaggcgaga agcttggcat catagggccc
     4381 aatgggtcgg gcaagaccac tctgattaac tgcataacgg gcgtaattca ccccgaggaa
     4441 ggaaagatta cgttcgaggg tatcgatata tccaacatgc ccacttatcg ccgcgcgcgc
     4501 cgaggcatcg cacgcagttt ccagattcca cgtccttttg ttggcatgac ggtgattcag
     4561 aatctgctag ttcccctcga ctatgttgag agtggcaaaa gagacccggc cggcgcgcgc
     4621 gaaattctct ccacagtggg gcttaaggcg cgcgccgaag atcctgccga gtcgctgacc
     4681 catcttgagc tgcgcaagct cgaacttgcg cgcgcgctcg cagccgaacc tcggttacta
     4741 atcgccgatg aagtcatggc cgggctctcg gagccggaaa tagatgaggt gctggaaatc
     4801 cttttcaccc tgaattcacg tggcgttacg gtcattatga tcgagcacat catgcacgcg
     4861 attatgcgtt tttcgaaacg actcgtatgc atggagactg ggcgcatcat tgctgacgga
     4921 acgccgcaag aggtgggtag acaccccgaa gtcaggaggg tctattttgg taaataggct
     4981 ggtcgttgag aatttgtcgg ctggttatgg cgcggtatct gtactgcgcg gcgtttcgct
     5041 cgaaatccgc gagggcgagc tcgtcgcatt gcttggcact aatggcaatg gcaaaagcac
     5101 tctcttgaac gcgatcctcg ggctggtgcg gccgactggg ggctcggtcc gcttggaatg
     5161 ggacggcgag attaccgagc ttgccggcag tcccgctgaa aaaattgtcg aacggggcat
     5221 cgctctggtt cctgaaggcc gtcgtctgtt ccctcacttg acggtcgatg aaaatctgac
     5281 gcttggcgga tgcgggcgcc gtgcgcgtca caatctccgg cacaatctgg agttcgtctt
     5341 cgcggccttt cccgtgctgc aggaaaggcg acgtcagcca gccggaacga tgagcggtgg
     5401 acagcagcag ttgctggcga tcgcgcgggc cttgatgacg gagccgcgca tagttttgat
     5461 cgacgagcca tcggtcgggc tcgcccctat tgccgtcgat caagtgcttt cgacggtaag
     5521 ggagcttcaa gctaaccgcg ggcttacagt ccta
//
LOCUS       DATMGX010000068         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_18362, whole genome shotgun sequence.
ACCESSION   DATMGX010000068 DATMGX010000000
VERSION     DATMGX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18362"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..392)
                     /locus_tag="VNR65_01095"
                     /pseudo
     CDS             complement(111..392)
                     /locus_tag="VNR65_01095"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
     gene            497..1759
                     /locus_tag="VNR65_01100"
     CDS             497..1759
                     /locus_tag="VNR65_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HWJ17300.1"
                     /translation="MNENSVVSLRQKDEIDDPLTEILRTGARRLITRAVEVEFDTFLA
                     SNCDLALPDGRRRVVRHGHDPVRTIQTGIGPVKVQKPKARDRSAPEAGERIRFTSSIL
                     PKWARRTKSLDALLPALYLRGISAGDFQEVLTALLGKDAPNLSPAVIARLKGEWEDEY
                     QRWQKRDLSARRYVYVWADGVYLQARMEPQAECMLVLIGATPEGKKELIGFQTGMRES
                     AQSWKELLVDLKARGLSIAPEVAVGDGALGFWKALDEIFPSTRHQRCWLHKTLNVLDK
                     FPKSVQPNAHKDLREIWLAPDRATAETAIATFAEKYAPKYDKAVECLIKDRETLLTFF
                     DFPADHWDHLRTTNPIESVFATVRHRTVRMKGALSQATARLMVFKLVMAAAKTWRRLQ
                     GQSQLPKVIKGVKFRDGIEVAPETKSAA"
     gene            complement(1791..2366)
                     /locus_tag="VNR65_01105"
                     /pseudo
     CDS             complement(1791..2366)
                     /locus_tag="VNR65_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          560 a          736 c          774 g          506 t
ORIGIN      
        1 cgcaaaaagc tttgctcgat ccgtccccgg cgaatccctg atcgatgcga ggatgctctg
       61 atcgccgttt cggattccag cttggaactt cgtttgcaga tcggcgagac tcatgcggcg
      121 gcctcttggt tcgcgcgtgc ggccgctgca gcgacggttc gcgcgcgatc gagttccgcc
      181 atgagttcgt cgaagggcgg gaaattggcg tcacgctcga tcatcgtcga cacggatccg
      241 aagcggtgac aagtctcata atagagcgcc cagacttctt cgcagatcgg ctgatcatgc
      301 gtatcgatct tgtgcgttcc cacgtctgaa tggcccgcaa gatggaattg gaccactcga
      361 tcttttggaa tggtctcgat gtagcgcacc ggatcgaagc cagagctatg ctggaaactg
      421 ggtgacgggg tgaagaaggc ggcgtaacgg ggttggtgat caaaccaccc gaatccccaa
      481 gaaggagcgt tacgccatga acgagaatag cgtcgtctct ctccgacaga aagacgaaat
      541 cgatgatccg ctaaccgaaa ttctgcgaac cggcgctcgt cggttgatta cacgggcggt
      601 tgaggtcgaa ttcgatacct ttcttgcctc gaactgcgat ctggcccttc cggacggccg
      661 ccggcgcgtc gttcgccacg gacatgatcc ggttcgcacg atccagaccg ggatcggccc
      721 ggtcaaagtg caaaagccga aggcccgcga tcgtagcgcg ccggaggctg gcgagcgcat
      781 tcgcttcacg tcttcgatcc taccaaaatg ggcgcggcgc acgaagagcc tcgacgcgct
      841 cctgccggct ctctatctgc gcgggatttc ggcgggcgat tttcaggagg ttctcacggc
      901 cttgctcggc aaggacgcgc caaacctctc gccggcagtg atcgcccggc tcaagggcga
      961 gtgggaagac gagtatcagc ggtggcaaaa gcgcgatttg tcggcacgcc gctacgttta
     1021 cgtgtgggcg gacggcgtct atctgcaggc gcggatggag ccacaagccg aatgcatgct
     1081 ggtactgatt ggcgcgacgc ccgagggcaa gaaagagttg atcggtttcc agacgggcat
     1141 gcgggagagc gcgcagagtt ggaaggaatt gctcgtcgat ttgaaggcgc gtggcttgtc
     1201 gatcgcgcca gaggtcgccg tcggcgatgg cgcgctcggc ttctggaaag ctctcgacga
     1261 gatcttccct tcgacgcgcc accaacgctg ctggctgcac aagacgttga acgttctcga
     1321 caagtttccg aaatccgttc agcccaatgc gcacaaagac ctgcgcgaaa tctggctggc
     1381 gccggatcga gcaacagcgg agacggcgat cgcgacattc gccgaaaaat atgcaccaaa
     1441 atacgacaag gccgtcgaat gcctgatcaa ggatcgcgaa acactgctga cgttcttcga
     1501 ctttcccgcc gatcactggg accatctacg aacgacgaat cctattgaaa gcgtgttcgc
     1561 gacggtcagg catcgtacag tccgcatgaa aggcgcgttg tcgcaagcca ccgctcgcct
     1621 catggttttc aagctggtga tggcggcggc gaaaacttgg cgaagactgc aaggtcaaag
     1681 ccagttgccg aaagtcatca agggtgtcaa attccgtgac ggaatcgaag tcgcgcccga
     1741 aaccaaatcc gccgcttgat tcacgccgtc accgaaaatc cgccgtagct cgtgtggatc
     1801 ttatagggaa cggccgcaat caaggcttcc aggaaagcgg acgccgatgt tcggccggtc
     1861 tttttcacca actgcacgaa ggcaaatttg ctggtgcgat caatagcgac atagagatag
     1921 agcttccctt cggcggtctg cacctcggcg atgtcgatgt gaaagtagcc gatgggatag
     1981 gccttgaact tgcgcttctc gggcttgtcc ccctccacat ccggcaggcg gctgattccg
     2041 tgtcgttgca ggcagcgatg cagggacgag cgggtcaaat gcggaatcgt cgcctgcagc
     2101 gcatagaggc agtcatcgag cggcagcagc gtgtgccggc ggaaagcgac gacgatcgct
     2161 tcatcctcga ccgtcagcac cgtcgacttc ggtcgcttgg ggccggtcgg gcgatcggcg
     2221 accgaggctc ggctcttcca cttagcgacg gtcttctgat tgatcccgta gcgcttggcc
     2281 agagccctca ggctctcttg actatgttgt atcgctcgac ggaccgcctc tgtcgttgtg
     2341 gcgctcccgt gtagaacctg tcccatagcg catccctcca ttcaggagaa aataatgcac
     2401 catcaaaata cgggatcaaa cacctaagct ccgcgcagcg ccttgcacgt ggccggctag
     2461 aactgaaaat aaatgaaatc agctttttcg aaaatgccga agagcatgat gtcgtagaag
     2521 agcagcacgg ccgcagcaat tgcaacagag cgatgggcac gcaacctcgc caaaag
//
LOCUS       DATMGX010000069         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_254272, whole genome shotgun sequence.
ACCESSION   DATMGX010000069 DATMGX010000000
VERSION     DATMGX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254272"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1075..>2351
                     /locus_tag="VNR65_01110"
     CDS             1075..>2351
                     /locus_tag="VNR65_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HWJ17301.1"
                     /translation="MIRPSLFLFLLAPSAAFAQQSAIPPELENPRITGVNKEAAHATL
                     TPFPAEAAALRGGAGDAVFTKSLNGPWRFHWVKQPSERPTDFYKPDFPVSSWKEIEVP
                     SNWEMKGYGTPIYSNVPYPFKRDAPHVMEEPDDKTWTAYAERNPVGSYRRTFDIPQNW
                     RGREIFIVFDGVSSALVLYVNGERVGYSQDSRLPAEFDITRYVRPGVNTVAAEVYRWN
                     AGSYLEDQDIWRVSGIYRNVTLVARTPVHVRDFQVLTPLDAAYRDATLQVKASVRNFS
                     AAQASGTLEVKLFNARGSEAMKTAAATWSAAPNSEAAVELKQAVASPAKWSAEEPNLY
                     KLVLTLKNASGAVLESIPWEVGFRTVEIKGDQLLFNGRKIYLKGVDRHEFDPDTGQYM
                     TRERMVEDITLMKRNNINAVRTSHYPNAPEWYSL"
BASE COUNT          487 a          711 c          712 g          441 t
ORIGIN      
        1 cgcacacggc ccgaatcgag cacgcgtcga gcccgggctg gggtgcgagc cgttcgtcgc
       61 ggcggtcgcg tggcgcgtgg gcgtgccggc gggttcggag tcgtcaggct acgacggcaa
      121 ttgtacgcgg aggcgacgat acgaccagtg gcacaagttt ccgctatatg gccgtttttg
      181 ttcctttgac gggcacgaag acgcgtaaaa cgatgacagc tcaatgactt aatgcctgtt
      241 cgaggcctct gctccaggct gcgcgaatcg ccagcaaatg cgcgcagatc agggtaaccc
      301 gtcttctacc cttagcgtgc ttgactgtca acaaggagtc cctaatctat ttgaactcca
      361 cagggctgta tcgctggcag ggggctgtca acacgtacta tcagcggagc cgctttaacc
      421 ttgtcgatga gcaattcccc caggtccttc caagcctctc ggcgcgcctg accgtcatca
      481 aggggttccg gccaatggtt aatctccacc tcaacgaagt attcttgccc tggcgtgaga
      541 gtgccgtgct ggcgctcgcc aatcggtacc gtcagacgat tcaagcggtg caagattaca
      601 cccggttcca aggcgttaaa gaatggaaag tccggccaag atggcaacgg aggaagctcc
      661 gctgagttgt cctgggaaat tccacactga actccggcct taccttgggc agtcgcggat
      721 tttgccgaaa gactacatgg cgagagtagc gggtgaaagc cggaatcagg gatcggacaa
      781 gtgtctccgt cttatactca aggtcgagtg tgacatccgg gacgatttca cggcctgcgt
      841 atgcagatgt cggggatgct gaccagggct tttggagtac gtgcagacca aatccagggg
      901 tgtcctgcgg ggcaaacgct ctatgacatc gatgcaacca tgccgcccgg caccaccaaa
      961 gagcagtttc aggagatgct tcaaaacctg cttgtggaac gcttccatct agaatttcac
     1021 cgcgagagga ggaactttcc cgcataatgc taaaattgtc cgcggattaa aaacatgatt
     1081 cgtccctctc tttttctgtt cctgctcgcg ccctccgcgg ccttcgcgca gcagtcggcg
     1141 atcccgcccg aactggagaa cccgcggatt acgggcgtca acaaggaggc ggctcacgcc
     1201 acgctcacgc cgtttcccgc cgaagcggcc gcgctgcgcg ggggcgcggg ggacgccgtc
     1261 ttcaccaagt ccctgaacgg gccatggcga ttccactggg tgaagcagcc ctccgagaga
     1321 ccgacagatt tctacaagcc cgacttcccg gtgtcctcgt ggaaagaaat cgaagtgccc
     1381 tccaactggg agatgaaggg ctacggcacg ccaatctact ccaacgtccc ttatcccttc
     1441 aagcgcgacg ccccgcacgt gatggaagag ccggacgaca agacctggac ggcgtacgcc
     1501 gagcgtaacc ccgtaggctc gtaccgccgc accttcgata tcccgcagaa ctggcgcggg
     1561 cgcgagatct ttatcgtctt cgacggcgtg agttccgcgc tcgtgctgta cgtgaacggg
     1621 gaacgcgttg gctacagcca ggattcgcgc cttcctgccg agttcgatat cacgcgttat
     1681 gtgaggcccg gcgtcaacac cgtggctgcc gaggtctacc gctggaatgc cggcagctat
     1741 ctggaagacc aggacatttg gcgcgtgagc gggatctacc gcaacgtcac gctggtcgcg
     1801 cgcacgccgg tccacgtgcg cgattttcag gtccttacgc cgctggacgc ggcttaccgc
     1861 gacgccacgc tgcaggtgaa agcgagcgtg cgcaattttt cggccgcgca ggcttccggt
     1921 actctcgaag tgaagctctt caatgcgcgc ggctccgagg cgatgaaaac ggccgccgcg
     1981 acgtggtcgg ccgcgccaaa cagtgaagcg gcggtggaac tgaagcaggc tgtcgccagc
     2041 ccggccaagt ggtcggccga ggagccgaac ctgtacaaac tggtgctcac cctgaaaaac
     2101 gcttccggcg cggtgctgga gagcatcccg tgggaagtcg gattccggac cgtcgagatc
     2161 aagggcgatc aactgctgtt caacgggcgc aagatctacc tcaagggcgt ggaccggcac
     2221 gagttcgacc cggacaccgg gcagtacatg acgcgcgagc gcatggtcga ggacatcacg
     2281 ctgatgaagc gcaacaacat caacgctgtc cggaccagcc attacccgaa cgcgcccgag
     2341 tggtactcgc t
//
LOCUS       DATMGX010000070         4798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_155032, whole genome shotgun sequence.
ACCESSION   DATMGX010000070 DATMGX010000000
VERSION     DATMGX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155032"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VNR65_01115"
     CDS             <1..410
                     /locus_tag="VNR65_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWJ17302.1"
                     /translation="YEAMMRGIAVIRPGASTGDIGAAIQTFVEAQHMSVVRDFCGHGL
                     GRLFHDEPNIVHVGRPGEGIVFKPGMLFTVEPMINLGKPHVKMLSDGWTAVTRDRSLS
                     AQFEHTIGVTETGIEVFTYSPMGLHKPPYPGQV"
     gene            507..1268
                     /gene="radC"
                     /locus_tag="VNR65_01120"
     CDS             507..1268
                     /gene="radC"
                     /locus_tag="VNR65_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWJ17303.1"
                     /translation="MSNGFEESPLFELIALETGANVPPPQPPRPIPHYWGHRDRLRRR
                     FREGGAGALPDYELMELILFRAIPRRDVKPLAKEILTRFGSFAEAVTAPPERLMEIEG
                     VTDAVVTEIKVVQAAALRTSLSEVKKRPVLAAMSTVLSYLRSAMAFEDREQFRILFLD
                     RRNKLIADEVQTRGTIDHTPVYTREVMKRALELSASAIILAHNHPSGDPTPSRADIDM
                     TKQIVDVATKLGVVVHDHIILGRDGHLSMKSVGIF"
     gene            complement(1333..1569)
                     /locus_tag="VNR65_01125"
     CDS             complement(1333..1569)
                     /locus_tag="VNR65_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17304.1"
                     /translation="MNLVIIGDSDSTGKAMLHDMRVSYIRRERPRFRYDAPPSYREVA
                     FFAACLGVFAALLYLRFKWGVVIRLNEAAVLFAP"
     gene            1812..2141
                     /locus_tag="VNR65_01130"
     CDS             1812..2141
                     /locus_tag="VNR65_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17305.1"
                     /translation="MKRRLFALTIGAFALLPLGLSQPASAYDYYDGYRGASRLERDIG
                     HDRAKLDRDYAQHRYYSEREQQALRDGHHLGAWWFGMRRHHEERVIDNRRADLYRDHQ
                     RLERYTY"
     gene            3226..>4798
                     /locus_tag="VNR65_01135"
     CDS             3226..>4798
                     /locus_tag="VNR65_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWJ17306.1"
                     /translation="MRASIKLKLAVNFTILTALAAVVAWLGISSLQHLNTTLVGLVNG
                     PVVRLQHADELRLAIMNIAPAEKNMIMATTPEEVGKIGAELIKRRNLFLKKFETMQNI
                     ATDQDREKLIAVGPIWVRWTGLQDQERELMEQGRQAEARVMSARDAKAARDDLMRQLE
                     EINEVENGQLNKAKEQAAQQYDYAWQLLIGAAAAALLISAISGVLLSLSINRGLRRAV
                     TLADAVARGDLDQDVQASSNDEIKDLIDSLNRMTANLRVTAEMAEAIARGDLSVDPKP
                     LSEKDMLGLALREMTQKLRTVVSDALSAAGNVSSGSEELSSACQQLSAGANEQAASAE
                     EVSASMEEMAANIKQNADNAAQTEKIARQSSANAQASGDAVNRAVQAMQTIAEKITFV
                     QEIARQTDLLALNAAVEAARAGEHGKGFAVVASEVRKLAERSQTAATEISTLSGQTMT
                     AAREAGTMLVKLVPDIKKTAELVEEISAACREQDIGANQVNQAIQQLDKVIQQNAGAA
                     EEMSATSEALSGQAET"
BASE COUNT         1007 a         1424 c         1399 g          968 t
ORIGIN      
        1 cctatgaggc gatgatgcgc ggcatcgcgg tgatccgccc gggcgcctcc accggcgaca
       61 tcggcgccgc catccagacc ttcgtcgagg cccagcacat gagcgtggtc cgcgacttct
      121 gcggccacgg cctcggccgc ctgttccatg acgagcccaa catcgtccat gtggggcggc
      181 ccggcgaagg catcgtgttc aagccgggca tgctgttcac cgtcgagccc atgatcaacc
      241 tgggcaagcc tcatgtgaag atgctttcgg atggctggac ggcggtgacg cgcgacaggt
      301 cgctgtcggc ccagttcgag cacacaatcg gcgtcaccga gacgggcata gaggttttca
      361 cttacagccc tatgggtttg cacaaaccac cctatcccgg ccaggtctga aattcatatc
      421 aattttagct aaactgatta ttctctccgg ggacgatgcc gtgtataaag gcacctggat
      481 ttgtttgtgt tgcgttggcg atttccatgt cgaacggttt cgaagagagt cccctattcg
      541 agttgattgc gcttgaaaca ggcgccaacg taccacctcc gcaaccccct cgaccaatcc
      601 cgcattattg ggggcatcgt gaccgcctgc gaaggcgctt ccgcgagggc ggagcagggg
      661 cacttcccga ttacgagctc atggagctta tcctttttcg tgcaattcca cgccgggatg
      721 tgaaacccct tgcaaaggag atcctgacac gcttcggctc ctttgctgaa gccgtgaccg
      781 cacccccgga gcggttgatg gagatcgaag gcgtgacaga cgctgtcgtg accgagatca
      841 aggtcgtgca ggcggccgcg ctgagaacct cactaagcga ggtcaaaaag cggccggtgc
      901 tggcggcgat gtcaaccgtt ctgtcatact tacgctctgc aatggccttc gaggaccgcg
      961 agcaattccg catccttttt ctcgaccgcc gcaacaagct catcgccgac gaagtacaga
     1021 cgcgcggtac gatcgaccac actcctgttt atacgcgcga agtcatgaaa cgggcgctgg
     1081 agctttcggc gtctgcgatc atcctcgccc acaaccaccc ctcaggtgat cccacgccgt
     1141 ctcgggccga tatcgacatg acgaagcaga ttgtcgacgt ggcgacaaag cttggcgttg
     1201 tcgtgcacga tcacatcatc ctgggccgcg acggccatct cagcatgaag tccgtcggga
     1261 ttttttgagc gtggttcaat agccccactg agcaaaataa tcagggggac gccccttcca
     1321 acgggagggg atttacggtg cgaagagaac agcggcttcg ttcaggcgga tgaccactcc
     1381 ccatttgaag cgcaggtata gaagcgccgc gaagacacca aggcaggcgg cgaaaaaagc
     1441 gacctctctg taacttggcg gtgcatcgta tcggaaacgc gggcgctcgc gccgaatgta
     1501 gctgactctc atgtcgtgaa gcatggcttt gcccgtagaa tcagaatcgc cgatgatcac
     1561 tagattcatg ggcagcgact ttctccacaa ccccccgaag ggtgatgcgt gcacagaaac
     1621 gcaaaacgca tgcaccaagt atgacttttc atgacaaaat gtgactcgaa agacgcatac
     1681 ggtcagtctc attcaagaga tcacgaagtc gtgatgattc tgaacgaatt ctaaacggag
     1741 cctttcgcct gcgttcagcc agcaaaggct aagctctcat catcgtttcg agatggattg
     1801 agaggtatcc gatgaaacgc cgtcttttcg cactgaccat tggcgctttc gctcttctgc
     1861 cgcttggcct gtcgcagccc gcttccgcct acgattatta cgacggctat cgcggggcta
     1921 gccgcctcga gcgcgacatc ggacacgacc gagcaaagct cgatcgcgat tatgcccagc
     1981 atcgctatta cagcgagcgc gaacagcagg cgcttcgcga cggccatcat ctcggtgcct
     2041 ggtggttcgg gatgcgccgc caccacgagg agcgcgtgat tgataaccgc cgcgccgacc
     2101 tttaccgcga tcaccagcgg cttgagcgct acacctatta gcagttgcgt ttgttgcgtg
     2161 tcggttggtt gtctgtcttg cattcgttgc gtgaaggtct cgcgtgaatt tgcgtgtgtg
     2221 tcctgcgtca gttgcgttgg gtgttgcgtc agtggcgtga aggtcttgcg tagttgcgta
     2281 cgagtcttgc gtcaggcgtt tgcgttcgtt ggctgtcgtt gtgtcattcc gggccggcgc
     2341 ttgcgccgca gccggaaccg catccccccg ttcgtcccaa gtcccccaaa gcgttgtatc
     2401 gtttccccgc aggatcccgg aaaggcctag ccttctccgg gatctttcca aaacctgtgt
     2461 tgctccatcg atcccggatg agttctggcc ttcgcggatc gccccccgac ccggcttgca
     2521 ggctgagcag ctttttgtat gcaatctgcg aatgacggca tcttttgcca atttcttcct
     2581 gccacttgcg tccgctttcc ttgttctgaa aagatcctca gcgatttcgc cttgccgacg
     2641 agttaacgag agcgccagcc cgcttcagcc cttacaaatg tgaggaaaaa tagggaacgg
     2701 gacgccatcg ccgccgcctg tgccgcagct gttccagctg cgtgcaagcg ttaattcgcg
     2761 aagcctcaac atatttaacg gacttttgcg cgccgagtta atgagaaaat tccggcatta
     2821 aattctattt aatgggcact ctgttaagga taaaaagctg ttctttttca attggttatg
     2881 gcctgtcatc ggcggcccgc tcaaaggcac ggcggaccac ttttcgcgct ggatagacga
     2941 ttttttaagc atacatgcgg cgggaaataa atccggcgcg ctcctgttaa ccgtctttgt
     3001 attaaagctc gtcgcggcta cgaacggttt accctaaggc gtgcggcgag cgtcgtccaa
     3061 gatggcacgg ccggattggc gccctgacgc tgaaactgct tttttgcttt caattttgcc
     3121 ccttgcttca acgggatccc aacatcgacg ccgcatacct taagtttgag agcgtgcgaa
     3181 ccaagccacg cccatccgcc tgccgaaaag aggatgactg gacccatgcg cgcatccata
     3241 aagctcaagc tcgccgtgaa cttcacgatc cttactgcgc tggcagccgt cgtcgcttgg
     3301 cttggcatct cgagcttgca acacctcaat acgacgctcg ttggcctcgt caatggaccg
     3361 gtcgttcgtt tgcagcatgc agacgaactt cgactggcaa tcatgaatat cgccccggcc
     3421 gagaaaaaca tgattatggc cacgaccccg gaagaggtcg gcaagattgg tgctgagctg
     3481 ataaagcgcc gtaatctttt cctgaagaaa ttcgagacga tgcagaatat cgcgactgat
     3541 caagaccgag aaaagctcat cgcggtcggc cccatttggg tgagatggac gggtctgcag
     3601 gatcaagagc gggagctcat ggagcagggc cgccaggcgg aagcgcgggt catgtccgcg
     3661 cgcgatgcaa aggcggcgcg agacgacctt atgcggcagc ttgaggagat caacgaggtt
     3721 gagaatgggc agttgaacaa ggcaaaggaa caagccgccc aacaatacga ctacgcatgg
     3781 cagctgctga tcggagccgc cgccgccgcg ctcctgatca gcgccatctc cggcgtcctt
     3841 ctttcgctca gcatcaatcg cggcctgagg cgagccgtca cgctcgccga tgcggtcgcg
     3901 cgcggtgatc tcgatcagga cgtccaagcg agcagcaacg acgaaatcaa ggacctcatc
     3961 gattctctca accgcatgac tgcaaactta cgcgttaccg ccgagatggc cgaggcgatc
     4021 gcccgtggtg acctctccgt ggacccgaag cccctgtcgg agaaggacat gctcggcctt
     4081 gcgcttcgag agatgaccca gaagcttcgc accgtcgtca gcgatgccct gagcgccgcg
     4141 ggcaacgtat cgtccggcag tgaggaactc tcgtcggcct gccagcagct ctccgccggc
     4201 gccaacgagc aggcggcgtc tgccgaggag gtgtccgcct ccatggagga aatggcggcc
     4261 aacatcaagc agaacgccga caacgcggcc cagaccgaga agatcgcgcg gcagtcgtcg
     4321 gccaatgcgc aggcaagcgg cgacgccgta aaccgcgcgg tgcaggccat gcagacgatc
     4381 gccgagaaga tcaccttcgt gcaggagatc gcgcggcaga ccgaccttct cgcgctgaac
     4441 gcagccgtcg aggcggcgcg ggcgggcgag cacggcaagg gctttgcggt tgtcgcctca
     4501 gaggttcgga agcttgccga gcggagccag acggccgcca ccgaaatcag cacgctgtca
     4561 ggccagacca tgacggcagc gcgcgaggcg gggaccatgc ttgtgaagct cgtgcccgac
     4621 atcaagaaga cggccgagct tgtcgaggag atcagcgcgg cctgccgcga gcaggatatc
     4681 ggggccaatc aggtgaacca ggccatccag cagctcgaca aggtgatcca gcagaatgcg
     4741 ggtgcagccg aggagatgtc ggcgacttcg gaggcgctct caggccaggc cgagacgc
//
LOCUS       DATMGX010000071         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_254117, whole genome shotgun sequence.
ACCESSION   DATMGX010000071 DATMGX010000000
VERSION     DATMGX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254117"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..981
                     /locus_tag="VNR65_01140"
     CDS             241..981
                     /locus_tag="VNR65_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWJ17307.1"
                     /translation="MRIALLADVHANRQAFSACLRHAEERGAERHVLLGDFVGYGADP
                     VYAVDTAMELVSKGAVAVLGNHDAAINDPTIRINPEAQAAIDWTRGLLGVEERRFLAD
                     LPLTISEQDRLYVHADASEPGKWIYVLTVEEASRSLYSTKAQITFCGHTHKPGIFSMT
                     TAAKMTAFSPVTEFAVPLHGLRRWLVVLGSVGQPRDGIPAASYSMFDTIKKEITFYRV
                     PYDIEGAADAIRKARLPLFFAQRLSMGR"
     gene            988..>2078
                     /locus_tag="VNR65_01145"
     CDS             988..>2078
                     /locus_tag="VNR65_01145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWJ17308.1"
                     /translation="MAKPTLEPGDVLDGFLMEGLIHRGGMARLWSVKKPGIDMPLLMK
                     VPVLSEGQDPAAIVSFEMEQMIMPRISGLHVPQFIAEGDFAVHPYIVMERVPGKSLLM
                     RLPDLPLPYEEVAEIGWKIATALDDIHRQHVIHLDVKPSNVLFRPSGEAVLIDYGLSH
                     HLQLPDLMQEEFRLPYGTAPYMAPEQLLGARHDPRTDQFALGVLLYFFSTGIRPFGES
                     ERLSAMKKRLWEDPKPPRRLREDYPLWLQEIVLRCLEVEPAWRYPSMAQLAFELGNHD
                     QIKLTARSERLKVDTFWARMKRRWNPDIRETYTPRTVALPLPSAPIIAVAIDTSEGSK
                     ETNDALRESAARALTLLPSARLACVNVLK"
BASE COUNT          423 a          624 c          624 g          407 t
ORIGIN      
        1 gattcgtgag tcatgccatg ctagaatctg actcgattcc gcatggcaag cgttaagtta
       61 gcgcgtatgg gtctggcccg gccatgacag gtgagcgaaa attcggcgat gaacatccgg
      121 gtaatcaaga aagctttaca aaggaaactg ccgcgatcat cccactcgga tgtatgaaat
      181 caaaagaccg cgtgccccgt tttcgagtaa tctggcaaaa acaaacattg gggagctgaa
      241 atgcgaattg cactgctggc agacgtacac gcgaaccggc aagccttcag cgcgtgtctg
      301 cggcatgccg aggagagagg ggcggaacgc cacgtgctcc tcggtgactt cgttggatat
      361 ggcgccgatc cggtttatgc ggtcgacacc gccatggagc ttgtttcgaa gggcgccgtc
      421 gccgtgcttg gcaaccacga cgccgcgata aacgatccca ccatccgcat caacccggag
      481 gcgcaggccg ccattgactg gacgcgcggc ttgcttggcg tggaggagcg cagatttctc
      541 gcggatttgc cgctcaccat ttccgagcag gatcggctct acgtacatgc ggatgccagc
      601 gagcctggga agtggatcta tgtgctgacg gtcgaggaag cctcgcgaag cctgtattcg
      661 acgaaggcgc agatcacctt ttgcggccac acgcacaagc cgggcatctt cagcatgacg
      721 acggcggcga aaatgacggc gttcagcccg gtcacggaat ttgccgtgcc gctgcatggg
      781 ctgcgccgct ggcttgtggt gctgggttcg gtgggccagc cgcgcgacgg tatccctgcg
      841 gcgtcatact ccatgttcga cacgattaaa aaagagatca ccttctatcg tgtaccgtat
      901 gatatcgaag gagccgccga cgcgatcagg aaggcacggc tgccactgtt cttcgcgcaa
      961 aggctgtcca tgggaaggtg atgcgtcatg gcgaagccca cattagagcc aggcgatgtt
     1021 ctcgacggct ttctgatgga agggctgatc catcgtggcg gaatggcgcg gctctggagc
     1081 gtcaagaagc ccggcatcga tatgcctctt ctcatgaagg tgccggtcct cagcgaaggg
     1141 caggacccgg cggcgatcgt cagcttcgag atggagcaga tgatcatgcc gcggatcagc
     1201 ggcctccacg tcccgcaatt cattgcggag ggagatttcg cggtccaccc ctacatcgtc
     1261 atggagcggg ttccgggaaa atcgcttttg atgcgccttc cagacctgcc tttgccctat
     1321 gaagaggtcg cggaaatcgg ctggaagatc gcgaccgcgc tcgacgatat tcatcgccag
     1381 catgtgatcc acctcgacgt gaaaccaagc aacgtcctgt tccggccctc gggcgaagct
     1441 gtgctcatcg actacgggct gtcccatcac ctccagctcc ccgatctcat gcaggaggag
     1501 tttcgccttc cctatggcac cgcgccctat atggcgcccg agcagcttct aggcgcgcgg
     1561 cacgaccctc gcaccgatca attcgcactc ggtgttcttc tttatttctt ttccaccggc
     1621 atccggccgt ttggcgagag cgaacgcctg agcgcgatga aaaaacggct ttgggaggat
     1681 ccgaagcctc cgcggcgtct cagagaggat tatccgctct ggctgcagga gatcgtcctg
     1741 cgttgcctgg aagtggagcc ggcgtggcgc tatccctcca tggcgcagct cgccttcgaa
     1801 ctgggcaatc acgatcagat caagctgacc gcccgctcgg agcgcctgaa ggtcgacact
     1861 ttctgggcgc gcatgaagcg gcgctggaac ccggatatca gagaaaccta cacgccgcgc
     1921 acggtcgcgc tgcctctgcc atcggcaccc atcatcgccg tcgccatcga cacgagcgaa
     1981 ggctcgaagg agacaaacga cgcgcttcgc gaatccgccg cccgcgcctt gaccctctta
     2041 ccctcggcgc ggctcgcttg cgtgaatgtg ctgaaaca
//
LOCUS       DATMGX010000072         4150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_466365, whole genome shotgun sequence.
ACCESSION   DATMGX010000072 DATMGX010000000
VERSION     DATMGX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466365"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..475)
                     /locus_tag="VNR65_01150"
     CDS             complement(62..475)
                     /locus_tag="VNR65_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17309.1"
                     /translation="MGFDDEVDEVKREMQTAKLDPQARRETAGKLCQRLKEQISSKRL
                     DFAVGDENGDPIVQIKYKPDNEKLASVYVNEDGSITFQAAGATDAAEEEEEDAGYFPS
                     YVEYMDEEEFLEDAYDMLKVGIAEYELDREQRSSS"
     gene            complement(535..1221)
                     /gene="purQ"
                     /locus_tag="VNR65_01155"
     CDS             complement(535..1221)
                     /gene="purQ"
                     /locus_tag="VNR65_01155"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWJ17310.1"
                     /translation="MKSAVIVFPGSNCDRDVAVTLEVVTGKKPAMVWHGETSVPSVDL
                     IVLPGGFSYGDYLRSGAMAAHSPILRAVGAEAKRGVAVLGICNGFQILTEAGLLPGAL
                     MRNASLHYICRDVGLKVERIDTPFTNAYAPGEQVTMPIAHKDGSYFADEETLDRIEGE
                     GQVALRYAPRAETGDSGNPNGSRRDIAGVVDCTHRIMGLMPHPERAADAALGGTDGRR
                     LFESLTRGLN"
     gene            complement(1218..1457)
                     /gene="purS"
                     /locus_tag="VNR65_01160"
     CDS             complement(1218..1457)
                     /gene="purS"
                     /locus_tag="VNR65_01160"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWJ17311.1"
                     /translation="MKARITVTLKNGVLDPQGRAIANALHSLGFDNVEDVRQGKLIEV
                     QLKAMDEASAKGELDAMCRQLLANPVIENYSVELL"
     gene            complement(1593..2369)
                     /gene="purC"
                     /locus_tag="VNR65_01165"
     CDS             complement(1593..2369)
                     /gene="purC"
                     /locus_tag="VNR65_01165"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWJ17312.1"
                     /translation="MNNRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHERF
                     EGKGVLNNRISEYIFSRLNEIGVPTHFIRRLNMREQLIREVEIIPLEVVVRNVVAGSL
                     CARLGLEEGMPLPRSIIEFYYKSDKLNDPMVTEEHITAFGWAAPQEIDEIMALALRIN
                     DFLTGLFLGIGIRLVDFKVEFGRIYENNEQVRIVLADEISPDSCRLWDIETKDKLDKD
                     RFRQDMGGLLEAYSEVAKRLGILVENPTPKPKGPVLVQSK"
     gene            2681..3004
                     /locus_tag="VNR65_01170"
     CDS             2681..3004
                     /locus_tag="VNR65_01170"
                     /inference="COORDINATES: protein motif:HMM:NF018993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HWJ17313.1"
                     /translation="MTTFDEREKGFEAKFARDEALRFKATARRNRYLGLWAAGLLGLS
                     GPEAELYAKDVIRADFLEPGDEDVIAKVMTDFKAKGVAVTDAQLRTKLHELTGQALAD
                     LEAGK"
     gene            complement(3042..4079)
                     /locus_tag="VNR65_01175"
     CDS             complement(3042..4079)
                     /locus_tag="VNR65_01175"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ17314.1"
                     /translation="MTTAIQTDAFIIGAGPVGLFAVFELGLLDVKAHVIDILDKPGGQ
                     CAELYPEKPIYDIPGLPVVTGQELTDRLLEQIKPFEPVFHFGQRVDSLRRVEGGFEVE
                     TDIGSRFFAKVIVVAAGGGSFTPKRPPLAGIETYEGKSVFYSVRKMDAFRDKKVVVVG
                     GGDSALDWTLNLQPVAKSVTLIHRRDEFRAAPDSVNKMRALVSEGHLKFILGQVTKLH
                     GAGGELEALTVRGPVDGGSDQEFEVEAERFLPFFGLTMKLGPIANWGLNLHENLIAVD
                     TEKFETSEPGIFAIGDINWYPGKLKLILSGFHEAALMAQQAHRYVYPGKRVVFQYTTS
                     SSSLQRKLGIL"
BASE COUNT          889 a         1248 c         1137 g          876 t
ORIGIN      
        1 gaactctgcc taaacaaaag actctaatca gacattcgtt ggctgaccaa ggaacgtctg
       61 cttaagagga agagcgctgc tcgcggtcga gttcatactc ggcgatgccg actttcagca
      121 tgtcgtaggc atcttcgagg aattcctcct cgtccatgta ctcgacatag ctcgggaaat
      181 acccggcgtc ctcttcctct tcctcggccg cgtcggttgc gcccgcagcc tggaaagtga
      241 tgcttccatc ctcgttcaca tagacggacg caagcttttc gttgtccggc ttgtacttga
      301 tctggacgat gggatcgccg ttttcgtcgc cgaccgcgaa gtcgaggcgc ttactgctga
      361 tctgctcttt caaacgctgg caaagcttgc ctgccgtctc gcgcctcgcc tgcgggtcga
      421 gcttggcggt ctgcatttcg cgttttactt catcaacttc gtcgtcgaaa cccatgtggc
      481 gatcctttcg ctagaacctt ttgaccctta aacgccagat ctgcaacgcc ttgatcaatt
      541 caaccctcgt gtcagagact cgaacagacg gcggccatcc gtgccgccaa gagcggcatc
      601 agcggcgcgt tcgggatgcg gcatgaggcc catgatacga tgagtgcaat cgacaacccc
      661 agcaatgtct cggcgggagc cgttgggatt accgctatcc ccagtttccg ccctgggagc
      721 gtatcgcagc gcaacctgcc cttccccttc gatgcgatcg agggtttcct cgtcggcgaa
      781 gtaggagcca tccttatgtg cgatcggcat cgtgacctgc tcgccgggag cataggcatt
      841 ggtaaagggc gtgtcgattc tctccacctt caacccaacg tcgcggcaaa tgtaatgaag
      901 agaggcgttg cgcatgagcg cgcccggcag aagcccggct tcggtcagga tctgaaagcc
      961 gttgcagata cctagaactg ccacgccacg tttggcctcg gcaccgaccg cgcgcaagat
     1021 cggcgaatga gcagccatcg cgcccgaacg aaggtagtct ccataggaaa acccgcctgg
     1081 taggacgatc aaatcgaccg agggtacaga cgtctccccg tgccaaacca tggcaggctt
     1141 cttccccgtg accacttcga gcgtcaccgc gacatcgcga tcgcaattcg atccggggaa
     1201 gacgataacg gcgcttttca caacaactcc acgctgtaat tctcgatcac ggggttcgcg
     1261 agaagctggc ggcacatggc gtccagttca cccttcgccg aagcctcgtc catcgccttg
     1321 agttgcactt cgatgagctt gccctggcgc acatcctcca cattgtcgaa gccgagcgaa
     1381 tgaagggcgt tcgcgatggc ccgaccttgc gggtcgagca ccccgttctt caatgtaacc
     1441 gtgatgcgtg ctttcatcgc gcgcgcctcc acactcctga tcggcctgag cagagttcgc
     1501 aaaagtggcc ttcccggttt tgcgaaaaaa ctctgcgaaa acaaaagatc tacgcgggca
     1561 ttcgttggtt gaccaaggaa tgcctgcgta gcctatttcg attggacaag caccggtcct
     1621 tttggcttag gcgttggatt ctccacaaga atgccgagcc gcttggccac ttctgaatag
     1681 gcttcgagca agcctcccat gtcctggcgg aaacgatcct tgtcgagctt atccttcgtt
     1741 tcgatgtccc agaggcggca gctgtccggg ctgatctcgt cggcaagcac gatgcgaacc
     1801 tgttcgttgt tctcatagat ccgcccgaac tcaaccttga agtccacgag cctgatgccg
     1861 atcccgagaa acaggccggt caggaaatcg ttgatgcgaa gcgccagcgc cattatctcg
     1921 tcaatctctt gcggcgcggc ccagccgaag gccgtgatat gttcctccgt caccatcgga
     1981 tcgttcagct tgtcggattt gtagtagaat tcgataatgg accggggcag cggcatgcct
     2041 tcttcaaggc caagccgcgc gcaaagcgac cctgccacga cattgcgaac cacaacctcg
     2101 agcgggatga tctccacctc tcgaataagc tgctcgcgca tgttgaggcg gcggatgaag
     2161 tgcgttggca cgccaatctc gttgaggcgg ctgaaaatat actcggaaat gcgattgttc
     2221 aaaacgccct tcccctcgaa acgctcatgc ttcttggcgt tgaacgcggt cgcgtcgtct
     2281 ttgaagtgct gtatgagcgt gccgggttcc ggcccttcat aaagaacctt cgcctttcct
     2341 tcatagatgc gacggcggcg gttattcatt gggaaacccc cttctcgtga aggaaacaag
     2401 caaagcggct gagacttgga tcgtacttca agcgcatatt tacgtcattg tcacgcgttc
     2461 ccgcaaaagt gcgagcggcg ttttcgctga atctgattgc ggccaagcta tttaccttcc
     2521 tcaccggccg ctccaggatt agccgagaag ctctgtagag gtagcgcaaa tgaacgctat
     2581 ccatggtatg ataaccatgc aaagcttgca ctaaaagatg gctccgcggg tctttcgatt
     2641 aaaggccccc cgatcgcaga ccatggaggc agttcccgag atgacaactt tcgacgaacg
     2701 agagaaaggg ttcgaggcga agttcgcccg tgacgaggca ctccgcttca aggcgaccgc
     2761 gcggcgcaat cgctatctcg gcctttgggc tgcggggttg ctcggtcttt caggccccga
     2821 agcggaactc tacgcgaagg atgtcattcg cgctgacttc ctcgagcccg gcgacgagga
     2881 cgtgatcgcg aaagtcatga cggacttcaa ggcgaagggc gttgcggtga ccgatgcgca
     2941 acttcgcacc aagcttcacg aactgacggg gcaagctctc gccgacctcg aggctggcaa
     3001 ataggcttcg cctgcacaaa ccggcgccac ggcctcccag gtcacagaat gccgagtttc
     3061 cgctgaagac tcgacgacga cgtcgtgtac tgaaaaacca ccctctttcc gggatacaca
     3121 tagcgatgcg cctgctgcgc cataagcgca gcttcatgaa aaccggagag gatgagcttg
     3181 agcttgccgg gataccagtt gatgtctccg atggcaaaaa tgccaggctc cgatgtctcg
     3241 aacttctcgg tgtccacggc aataaggttt tcgtgcagat tcaagcccca gttcgcgata
     3301 ggcccgagtt tcatggtgag gccgaagaat ggcagaaacc tctcggcctc gacctcgaac
     3361 tcctggtccg aaccgccgtc aacgggtccg cgcacggtca gcgcttcaag ctcgccgccc
     3421 gcgccatgga gcttcgtcac ctgcccaaga ataaatttca ggtgcccctc ggaaacaagc
     3481 gcgcgcatct tattgaccga gtccggcgcg gcgcgaaact cgtcgcggcg atggatgaga
     3541 gtcacgcttt tcgcgacagg ctgaaggttc aaggtccagt caagcgcgga gtcgccgccg
     3601 ccgacaacca caaccttctt gtcccggaag gcatccattt ttcgaactga atagaatacc
     3661 gacttcccct cgtaagtctc gatgccggcg aggggtggcc gttttggcgt gaacgatccc
     3721 ccgcctgcgg caacaacgat aactttggcg aagaaccgcg accctatgtc ggtctcgacc
     3781 tcgaaaccgc cttcgacgcg ccgcagcgaa tccacgcgct gaccaaagtg aaagaccggc
     3841 tcgaaaggct tgatctgttc aaggagccga tccgtcagct cttgcccggt cacgacagga
     3901 agccccggaa tatcgtagat cggcttctcg ggataaagct cggcgcattg gccacccggc
     3961 ttatcgagaa tgtcgatgac atgcgctttc acatcgagca gcccaagctc gaagaccgcg
     4021 aacaggccca cgggacccgc cccgatgata aacgcgtcag tttgaatggc tgttgtcatg
     4081 agactttatg gaatggatta aaaaccaacg gcgacggcat ccgccgtccc gcttaatttt
     4141 ggtgctcggg
//
LOCUS       DATMGX010000073         3099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_253944, whole genome shotgun sequence.
ACCESSION   DATMGX010000073 DATMGX010000000
VERSION     DATMGX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253944"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VNR65_01180"
     CDS             <1..221
                     /locus_tag="VNR65_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWJ17315.1"
                     /translation="SLIDGLGVRSPAGVAFRYTFVVDPENTIQHVYATNLNVGRNPAD
                     TLRVLDALQTEELCPCNRSVGGATLKAA"
     gene            310..837
                     /locus_tag="VNR65_01185"
     CDS             310..837
                     /locus_tag="VNR65_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWJ17316.1"
                     /translation="MSIEALKDRIGSFAKDVRLNLSSMLSDETLGEQTKYGLFLAAAL
                     AARNADLVAAFEAEAANRLSPAALDAAKAAASIMAMNNIYYRFLSLASNKDYSSKPAR
                     LRMNVIANPGVDKNDFELWSLAVSAINGCGTCIDAHEKALQKNGASAEAIQTAVRFAA
                     IIASVAVALEASTPA"
     gene            1104..1451
                     /gene="csgH"
                     /locus_tag="VNR65_01190"
     CDS             1104..1451
                     /gene="csgH"
                     /locus_tag="VNR65_01190"
                     /inference="COORDINATES: protein motif:HMM:NF041112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli-like amyloid fiber formation chaperone
                     CsgH"
                     /protein_id="HWJ17317.1"
                     /translation="MTSAAIAQSASAWIELKPVSGRNMVEVVGHALATDAVGGMDFTF
                     TLRRQNKGNTSSTRQSGRFDLAPGETKNLSSTSINMEPGDELTIELKLLDHGAEVSSA
                     TVTSKSTGGGQTL"
     gene            complement(1622..2419)
                     /locus_tag="VNR65_01195"
     CDS             complement(1622..2419)
                     /locus_tag="VNR65_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWJ17318.1"
                     /translation="MVQGITELMPISSTAHMRLVPWALGWPDPGSAFSAAMQLAALFA
                     VVSYFWSDVRGIVFGSLAAVVRGKFRDRNLQFALAIVLGTIPIVVLGLLLAPVLNACN
                     SPLRSIWVIGWACVVMSLLLALSELTASHKRKMERWTFLDAIVVGLSQAGALIPGVSR
                     SGSTLTAALFAGFNRADAARFSFLLGLPAITGAGLKELFELHKAGLDAHGWSVLAVGL
                     VVASISAFAAIWSLLRILERFSAWPFVVYRAVIGLVLVGGVYLGWFS"
     gene            2686..2943
                     /locus_tag="VNR65_01200"
     CDS             2686..2943
                     /locus_tag="VNR65_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17319.1"
                     /translation="MRLGKPAEAVPNDNPDVLPGAACSLINRPGTVCAVHACWEMESS
                     HKVIMPMKSSPKLQHRTIVRAVEAQPHLELDKGQETQPWER"
BASE COUNT          693 a          942 c          879 g          585 t
ORIGIN      
        1 gttcgctcat tgacgggctc ggcgtgcgct ctccggcagg tgtcgccttc cgctacacat
       61 tcgtcgtcga tcctgagaac acgatccagc acgtctatgc gacgaacctt aacgtgggcc
      121 gcaatcccgc tgatacgctt cgcgttctcg acgcgctgca gaccgaagag ctttgcccct
      181 gcaaccgctc ggtcggggga gccacgctga aggccgcgta gcgctgcatt cgtcaaatcc
      241 ttgccgcttc gaatgtcggc cttccgacat tccaagcagt gctttgtcac gtttcaaaca
      301 cggatgatca tgtcgattga agccctgaaa gatcgcatag gaagctttgc gaaggacgtt
      361 cggctcaatt tgtcctcgat gctttccgat gagacgcttg gagagcagac gaaatacggg
      421 ctgttccttg ccgctgcgct tgctgccagg aatgcagacc tcgtcgcagc cttcgaggcc
      481 gaggcggcga accggctttc tcccgcagcg ctcgatgcgg caaaggctgc ggcctcgatc
      541 atggccatga acaatatcta ctaccgcttc ctgtcgctcg cttcgaacaa ggactattcc
      601 agcaaaccag cccgattgcg gatgaacgtc atcgccaatc caggcgtcga caagaacgat
      661 ttcgagcttt ggtcgcttgc cgtgtccgcc atcaatggct gcggcacatg tatcgacgcc
      721 catgaaaagg cgcttcaaaa gaacggtgct tcagcggaag caatacagac tgccgtacgg
      781 tttgcggcca tcattgcatc ggtggccgtt gcccttgagg caagcacccc tgcctaatcg
      841 ccttggctca gccagcgaag ccgcacccgc aacgccctaa attccaacaa gtcacttaaa
      901 ggctttgcgg atctttgggg atgatttctg acgcgggttt ctttttatgc ggcgttagtg
      961 ataaggactc tgcaatcttt ggttgagagt tacaggcgta atcgatgaat ttcttttcaa
     1021 tgcctcaaag cgggaatgcg gcttgtgcat ctcgaaggcg caggtggcgg cttgccgcct
     1081 tatccttgat atcgggagcc ctcatgacaa gcgcagcgat tgcacaatcg gcctcggcgt
     1141 ggatcgagct caaaccggtg tccggccgta acatggtgga ggttgtgggc catgcgcttg
     1201 cgacggacgc ggtcggcggc atggatttta cttttacgct tcggcgtcag aataagggca
     1261 acacctcaag cacccggcag tcgggccgtt tcgacctcgc tcccggtgag accaagaatc
     1321 tgtcgtcgac gtccatcaat atggaacctg gcgatgaact cacgatcgaa ctcaaactcc
     1381 tggatcatgg cgccgaggtt tcgagcgcga ccgtaacctc gaagagcaca ggcggcggac
     1441 aaacgcttta gcaacgccgt tccgtcacgt cctgccagag ttgtctctct ttattctgcc
     1501 agacgcaggc gcaaccctta aggcgcctag tcgaaatctt cggggtgtcg gacttcctac
     1561 aatcccggac cttgtgatct gcgaacatgg gcacagaggc gtttgagctt tggaggcctc
     1621 ctcaactgaa ccagccgagg taaacgccgc ccaccagcac taatccgatg accgcgcggt
     1681 agaccacgaa cggccaagcc gagaagcgct cgagaatgcg caagaggctc cagatcgccg
     1741 cgaaggccga aatactggcg actacgaggc ccaccgcaag cacactccag ccatgcgcat
     1801 cgaggccggc tttgtgcaac tcgaaaagct ccttcagccc cgctccggtg atggcgggca
     1861 ggcctagcag gaaggagaag cgcgcggcgt cggcacggtt gaagcctgcg aacagcgccg
     1921 ccgtcagcgt cgaaccggaa cgcgagacgc cgggaattaa cgcgccggcc tgggagagac
     1981 cgacgacgat cgcgtcgagg aaggtccagc gctccatctt gcgcttgtgg ctggcggtca
     2041 actcagacag cgccaggagt agcgacatca caacgcaggc ccagccgatg acccagatgc
     2101 tgcgcagcgg cgagttgcag gcattgagaa caggcgcaag aagcaagccc agcaccacga
     2161 tcgggatggt ccccagcacg attgccagcg cgaattgaag attgcggtcg cgaaacttac
     2221 cccgcaccac ggcggcaagc gagccgaaga caataccgcg cacgtcgctc cagaaatagc
     2281 tcaccacggc gaaaagagcc gcaagctgca tggccgcaga aaacgcggaa cccgggtccg
     2341 gccagcccag cgcccagggc accaggcgca tatgcgcggt tgaagaaatc ggcatcagct
     2401 cggtgatgcc ctgcaccacg cccaaaaacg aaaccttagc atagccaagg cttacgaagc
     2461 ccgtatcgag gccattcgtg cacgcagcgg ccattgtccc tcgccccaga aaagctgttg
     2521 gtcgaacctt aagcagaaat ccatagcaac ggacgcccga aagccccggc gagcgggttg
     2581 cacgcacagg cgcccgttaa atgtcactaa cgcttgcgcc tatcggatgc ctgttttcgc
     2641 gggcatttcg aatgcgtacg ccaggaggtc tgcttagcag cacctatgcg cctgggcaag
     2701 cctgcggagg ctgtgccaaa tgataatcca gatgtgctgc ccggcgcggc ttgctcgctc
     2761 atcaaccggc ctggaacagt ttgcgcggtt cacgcttgtt gggaaatgga atcctcccac
     2821 aaggtgatca tgcccatgaa atcctctcca aaattgcagc acagaacaat tgtccgcgcc
     2881 gtcgaagctc agccgcatct cgaactcgac aaggggcagg agacacagcc ctgggaacgt
     2941 tgacggacct cccgatctcg ctcgacgcac agaggcgtgt gcagagcatt gaagctttaa
     3001 atcagctgct ctccgacacg atgacgctga gggacatgta caagaagcat cattggcagg
     3061 tgagcggccc aaccttctac cagcttcatc ttctcttcg
//
LOCUS       DATMGX010000074         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_279372, whole genome shotgun sequence.
ACCESSION   DATMGX010000074 DATMGX010000000
VERSION     DATMGX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279372"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VNR65_01205"
     CDS             <1..498
                     /locus_tag="VNR65_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17320.1"
                     /translation="NSKMATPGIVGARHYGLAQEIRRTFAQYAQLKDIISMLGLEQLS
                     PEDRNVVARARRLERFLTQPFFTTKQFTGLDGKLVSLEDALDGCERILRDEFKDYPES
                     ALYMIGAINEAKGKVKPSPSTAEPKSKAKADLTPAAKATPEPHPIPGAKPQPTLEHAG
                     DAHES"
     gene            488..877
                     /locus_tag="VNR65_01210"
     CDS             488..877
                     /locus_tag="VNR65_01210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HWJ17321.1"
                     /translation="MNLKVLLPFQIFVEKVGVSRIVAETHEGSFGLLPHRLDCVAALA
                     PGILIFETKIEAEAYVAVDEGVLVKTGSDVLVSVRNAIAGTDLGQLREAVQREFLHLD
                     EQEKNVRSVLAKMEGDLMRRMATFHNA"
     gene            870..1208
                     /locus_tag="VNR65_01215"
     CDS             870..1208
                     /locus_tag="VNR65_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HWJ17322.1"
                     /translation="MPDEPQNKLLKPKLALSEEVGAKAVRKLRARRHSAQGVWFGLGM
                     MGLIGWSVAIPTLLGAALGLWLDRHYPGRHAWTLALLVAGLAIGCFNAWHWVTKEDKA
                     MRDEREDGDE"
     gene            1201..1515
                     /locus_tag="VNR65_01220"
     CDS             1201..1515
                     /locus_tag="VNR65_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HWJ17323.1"
                     /translation="MNEVLLLALALLAGMLLGAIFFGGLWWTVRKGVSSEQPAFWFFG
                     SLLLRMGIALAGFYFVSGGQWERLLLCLVGFVIARLVVTWLTRSSGDNQSVPVQEARY
                     AP"
     gene            1505..2200
                     /locus_tag="VNR65_01225"
     CDS             1505..2200
                     /locus_tag="VNR65_01225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWJ17324.1"
                     /translation="MRLSPDEMIFWQYGLFKLNGTIVFTWALMFVLAVGSKIITRKLS
                     TGLERSRWQSLLEIVVTGIEKQIEDVGLRHPEKYIGFLGTLFLFVALASLCTIIPGYE
                     PPTGSLSTTAALALCVLVAVPFFGIEEQGLGGYLKSYVEPTVIMLPFNIISELSRTLA
                     LAVRLFGNMMSGTMILAILLTITPFIFPIAMSVLGLLTGMVQAYIFSILAAVYIASAT
                     RIRKPAPELDVKR"
BASE COUNT          429 a          629 c          698 g          495 t
ORIGIN      
        1 aattccaaga tggccacgcc gggtattgtc ggggcacggc attacggttt ggcgcaagaa
       61 atccggcgaa cgttcgcgca atacgcgcaa ctcaaggaca tcatctccat gctgggtctt
      121 gagcagcttt cgccggagga ccgcaacgtc gtcgcccgcg cccgccgcct agagcggttc
      181 ctgacccagc cgttttttac gacgaagcag tttaccgggc tcgacgggaa actcgtcagc
      241 ctggaggacg cattggacgg ctgtgagcga atcctgcgcg acgaattcaa ggactatccg
      301 gagagtgcgc tctacatgat tggggcgatc aacgaagcca agggaaaagt gaagcccagc
      361 ccgtccacgg ctgaacctaa gtcaaaagcc aaggccgacc tgaccccagc ggccaaagct
      421 acaccagagc cgcatcctat acctggggca aaacctcaac cgacgctcga acatgccggc
      481 gacgctcatg aatcttaagg ttctcttgcc ctttcaaatc tttgtcgaaa aggtcggcgt
      541 gtcccgcatt gtcgcggaga cgcatgaggg ctcgtttgga ctcttgccgc accggctgga
      601 ttgcgtagcg gcgctcgcac cgggaattct gatctttgag acgaagattg aggccgaggc
      661 ttacgtggcc gtggacgaag gtgtgctggt gaagaccggc tcggacgtgc tcgtttccgt
      721 ccgtaacgcc attgccggaa cggatctcgg ccaattgcgt gaggcggtgc agcgcgagtt
      781 tctgcatctg gacgagcagg agaagaatgt gcgctcggtg ttggcaaaaa tggaaggcga
      841 tctcatgcgc cgcatggcga cgtttcacaa tgcctgacga accccaaaac aagctcttga
      901 agccgaagct ggcgctcagt gaggaggtcg gtgcgaaggc ggtgcgcaag ctcagggcgc
      961 ggcgtcactc cgcgcagggc gtttggttcg gcttgggcat gatggggctg attggctggt
     1021 cagtggcaat tccgacgctg ctcggcgcag cgctcggtct gtggctggac cggcactatc
     1081 cgggaaggca tgcctggacg ctggcgctgc tggtggccgg gctcgcgatc ggctgcttca
     1141 atgcgtggca ttgggtaacg aaagaagaca aagccatgcg agacgaacgg gaggatggcg
     1201 atgaatgagg ttttgctgct ggcgctggca ttgttggcgg gcatgttgct gggcgccatt
     1261 ttctttggcg gcctttggtg gacggtgcgc aagggtgttt cgtctgagca gccggcgttt
     1321 tggttcttcg gcagcctgtt gctgcggatg ggcattgccc tggccggatt ttactttgtt
     1381 tccggcggtc agtgggagcg gttgctgctt tgtctcgttg gatttgtcat cgcgcgcttg
     1441 gtggtgacgt ggctgacccg gtcgtccgga gataaccaaa gcgtcccggt acaggaggcc
     1501 agatatgcgc cttagtcccg atgagatgat cttctggcaa tacgggttat tcaaattaaa
     1561 cggtacaatc gtgttcacgt gggcactgat gttcgtgttg gccgtcggtt caaagatcat
     1621 cacgcgcaag ctttccacgg gtctggaacg ttcccgttgg cagagccttc tggaaatcgt
     1681 cgtcaccggt atcgagaagc aaatcgaaga cgtcggcctc cgccacccag agaagtatat
     1741 cggctttctc ggcactcttt ttctgttcgt tgccctggcc agcctctgca ccatcattcc
     1801 cggctacgag ccgccgacgg ggtcgctttc gaccacggct gcactcgcgc tgtgcgtttt
     1861 ggtggccgtg ccgtttttcg gcatcgagga gcaggggctt ggcggctacc tcaagtccta
     1921 cgttgagccg acggtcatca tgctgccgtt caacatcatc agtgagcttt cacgcacgct
     1981 ggccctcgcg gtccgcctgt ttggcaacat gatgagcggg acgatgattc tcgcgatctt
     2041 gctgaccatc acgcccttca tcttcccgat cgccatgagc gtgctcggtc tgctcaccgg
     2101 catggtgcaa gcctacatct tcagcatcct ggctgcggtt tacatcgcgt ccgccacgcg
     2161 tatccgcaag cctgcgcctg aactcgacgt aaaacgttaa agcacgaacc ccggctcaag
     2221 aggaacgact atggatagca taacgctcat c
//
LOCUS       DATMGX010000075         5643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_466012, whole genome shotgun sequence.
ACCESSION   DATMGX010000075 DATMGX010000000
VERSION     DATMGX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466012"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..752)
                     /locus_tag="VNR65_01230"
     CDS             complement(264..752)
                     /locus_tag="VNR65_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HWJ17325.1"
                     /translation="MTASFCHCPLEAIIFDLNCTMLDSAAQVAAAINVRFRRCGLERV
                     LAGEIARHVGWGSSELIKSAFASRGAILSHERIIAQEKIYLANYASIAERDIVFFPGA
                     INLIGALAEPERRLGICTNKQAGIAPVLEKVKLLPMFGAIIGGDSGFGLKPSLATLYA
                     VL"
     gene            complement(755..1420)
                     /gene="rpe"
                     /locus_tag="VNR65_01235"
     CDS             complement(755..1420)
                     /gene="rpe"
                     /locus_tag="VNR65_01235"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWJ17326.1"
                     /translation="MHQPIIIAPSILSADFARLGEDVRDVMAAGADWVHLDVMDGHFV
                     PNITFGPAVIQALRPHSDKVFDCHLMIEPVDFFLESFAKAGCDIITVHAEATKHLDRS
                     LQAIRSLGKKAGVSLNPHTPESVIKYVLDRLDLVLVMSVNPGFGGQLFIPAIVEKVRR
                     IKAMIGNRPIDIEVDGGVTSDTAPLVVNAGANVLVAGSAIFKGGKAAYAGNIAAIRVA
                     ERG"
     gene            complement(1425..2387)
                     /gene="cbbX"
                     /locus_tag="VNR65_01240"
     CDS             complement(1425..2387)
                     /gene="cbbX"
                     /locus_tag="VNR65_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HWJ17327.1"
                     /translation="MSDQQSNAMSIAPRDTLPAAVSLREELDAVGISDVLAQLDRELI
                     GLKPVKTRIREISSLLLIERVRRKLNLSTSSPTLHMSFTGNPGTGKTTVALRMADILH
                     RLGFVRRSHVISVTRDDLVGQYIGHTAPKTKEILKKAMGGVLFIDEAYYLHRPDNERD
                     YGQEAIEILLQVMENQRADLVVILAGYGERMDKFFASNPGFRSRIAHHIDFPDYADDE
                     LLSIAEMMLGEQNYRFSPDAREAFVHYIKLRKTQPLFSNARSIRNALDRIRLRQANRL
                     VSDLDRLLTADQIMSIEPSDVLASRVFAEAETDSVDQSRVEKGS"
     gene            complement(2517..2936)
                     /locus_tag="VNR65_01245"
     CDS             complement(2517..2936)
                     /locus_tag="VNR65_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HWJ17328.1"
                     /translation="MRVTQGCFSFLPDLTDEQISRQVQYCLDKGWAVNIEFTDDPHPR
                     NIYWEMWGLPMFDLKDAAGVMMELADCRRTYGDRYIRMSGFDSSHGWESVRISFIVNR
                     PKDEPGFRLERQEVVGRNVRYTTKSYGVDKPEGQRYG"
     gene            complement(2949..4409)
                     /locus_tag="VNR65_01250"
     CDS             complement(2949..4409)
                     /locus_tag="VNR65_01250"
                     /inference="COORDINATES: protein motif:HMM:NF003252.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HWJ17329.1"
                     /translation="MNEHSVTLHGKERYKSGVMEYKKMGYWEPDYTPKDTDIIALFRV
                     TPQDGVDPIEASAAVAGESSTATWTVVWTDRLTAAEKYRAKCYRVDPVPNSPGQYFAY
                     IAYDLDLFENGSISNLSASVIGNVFGFKPLKALRLEDMRFPVAYVKTFQGPATGIVVE
                     RERLDKFGRPLLGATVKPKLGLSGRNYGRVVYEALKGGLDFTKDDENINSQPFMHWRE
                     RFLYCMEAVNKAQAASGEIKGTYLNVTAGTMEDMYERAEFAKELGSVVIMIDLVIGYT
                     AIQSMAKWARRNDMILHLHRAGHSTYTRQRNHGVSFRVITKWMRLAGVDHIHAGTVVG
                     KLEGDPATTRGYYDICREDFNPMKLEHGVFFDQNWASLNKLMPVASGGIHAGQMHQLL
                     DHLGEDVVLQFGGGTIGHPMGIQAGATANRVALEAMILARNEGRDYLHEGPEILAKAA
                     QTCTPLKSALEVWKDVTFNYESTDMPDYAPTASVST"
     gene            complement(4465..5550)
                     /gene="fba"
                     /locus_tag="VNR65_01255"
     CDS             complement(4465..5550)
                     /gene="fba"
                     /locus_tag="VNR65_01255"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01521.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWJ17330.1"
                     /translation="MARITLRQLLDHAAENGYGVPAFNINNMEQGLAIMDAAATVDAP
                     VIIQASRGARSYANDIMMSKMIDALEEMYPQIPLCMHQDHGNEEATCATAIKYGFTSV
                     MMDGSLKADAKTAADYDYNVNITRRVVEMAHWIGASVEGELGVLGSLEHGGGEQEDGH
                     GVEGKVSHDQLLTDPDQAVDFVRATKVDALAIAMGTSHGAYKFSRKPDGDILAMKVVE
                     EIHRRLPNTHLVMHGSSSVPQSLQDIFNKFGGEMPQTWGVPVEEIIRGIKHGVRKVNI
                     DTDCRLAMTAAFRKVAAQTKSEFDPRKFLKPAMDAMRDLCRERFEQFGTAGNAAKIKV
                     IPLAEMAKRYRAGALDPRIGTMAAAAE"
BASE COUNT         1202 a         1657 c         1515 g         1269 t
ORIGIN      
        1 tccgattgcg accacgtcca atcaagatcg gcttctgcaa aggctgcatt gaaagacact
       61 cgatgcgcct cctctgtctc cgccagagtt ccgtccacat caaaaataag agcggcaatc
      121 atggtgcagc caccttgttt cttctgtcaa taatggtcgc aagctcggat agttcgccca
      181 attgatattc tggctcaaag ggagtgatcg gggttggata acccggatgc cgtacacgcc
      241 cgcaggttgg tcgctttaag gcgctacaaa actgcataca gcgttgctag cgacggctta
      301 agcccgaagc cgctatcacc accaatgatt gcaccgaaca tgggcagaag cttcaccttc
      361 tcaagtaccg gtgcaatccc tgcctgctta ttggtgcaga taccaagcct ccgttcgggt
      421 tccgccaagg ccccaatcag attgatcgca cccgggaaaa agacgatgtc gcgttccgca
      481 atcgacgcat aattggctag atagatcttt tcctgtgcga tgatccgctc atgcgacaga
      541 atagctcccc gcgaagcaaa cgccgatttg attagttcgc tcgaccccca acctacatgg
      601 cgcgcgattt ctcccgcaag aactctctca aggccacacc tacgaaaacg tacatttatt
      661 gcagcggcga cttgcgccgc tgaatcaagc atggtgcagt tgagatcaaa aatgatcgct
      721 tcaaggggac aatgacagaa tgaagcggtc atcgttatcc cctttccgca acacggattg
      781 cagcgatatt tccggcatac gcggctttgc cacccttgaa gattgcggaa ccggccacca
      841 gaacattggc gcccgcattg accacgagag gggccgtgtc ggacgtcaca ccgccatcaa
      901 cctcgatatc gattgggcgg ttacctatca tcgcttttat gcgtctcacc ttctcaacga
      961 tcgccgggat gaagagttgc ccgccaaaac cgggattgac gctcatcacc agtacgagat
     1021 caagccggtc caagacgtat ttgatcacgg attcgggcgt gtgaggattg agcgagacac
     1081 cagccttctt gccgagcgac cggatagcct ggagcgaacg gtcgagatgc ttcgtcgcct
     1141 cagcgtgaac tgtgatgatg tcgcaacccg cttttgcgaa ggattccaga aagaagtcga
     1201 ccggctcaat catcagatgg caatcaaata ctttgtcaga atgcggccgc aatgcctgga
     1261 tcaccgcggg cccgaacgtg atgtttggta cgaaatgccc atccatcacg tcgagatgaa
     1321 cccagtcagc gcctgctgcc attacatccc ggacatcttc accaagcctg gcaaaatctg
     1381 cagaaaggat ggacggtgcg atgatgatcg gctgatgcat ctccttagga tcctttctcc
     1441 actcttgatt gatcaactga atcggtctct gcttctgcga aaacgcggct tgcaaggaca
     1501 tcggacggct cgatagacat gatttggtca gccgtcagca atcggtcgag atcggagacc
     1561 agacggttcg cttggcgcag ccgtatgcgg tccagcgcgt tgcggatcga acgcgcgttt
     1621 gaaaaaagcg gctgtgtctt gcgcagcttg atatagtgaa cgaaggcctc tcgtgcatcc
     1681 gggctgaagc ggtaattctg ctcgccgagc atcatctcgg ctattgaaag caattcgtcg
     1741 tctgcatagt cggggaaatc aatatgatga gcgattcgcg agcggaaccc tggattgctc
     1801 gcgaaaaact tgtccatgcg ctcgccgtat ccggccagga tgacgaccag atccgcgcgc
     1861 tggttttcca tcacttgcaa gagaatttct atagcttcct gaccgtagtc gcgctcgttg
     1921 tcaggacgat gaagatagta agcttcatcg atgaacagca cgccgcccat cgcctttttc
     1981 aatatctctt tcgttttggg cgcggtgtgt ccgatgtatt gcccaacaag atcgtcccga
     2041 gtcaccgaga taacgtgact acgtctcacg aacccaagcc tgtgcaggat atcggccatc
     2101 cgcaacgcga ctgtcgtctt gcctgttcca ggatttccgg tgaatgacat gtgcaatgtc
     2161 ggcgaggacg tcgagagatt cagcttgcgg cgcacgcgct cgatcaacaa aagcgatgaa
     2221 atttctcgaa tgcgggtctt taccggcttg agtccaatca attcgcgatc gagctgtgcg
     2281 aggacatcgc tgattccgac cgcgtccaat tcttcgcgca acgaaaccgc tgcgggaagt
     2341 gtatcccggg gcgcgatgct cattgcgttg ctttgttgat cagacactgt cgcctcgctc
     2401 accttcagat aaaagaatcc ccgctgtccc agcggcacaa cggggacaag gcttccaagc
     2461 cactaaacgc attcagggga agatgcggat gacctggtat cagttttgct tacaggttag
     2521 ccgtagcgtt gaccttcggg cttatcgacc ccataggatt tcgtcgtgta gcgcacattt
     2581 cgcccaacca cttcctgacg ctcgagacgg aagcccggct cgtccttcgg ccggttgacg
     2641 atgaaggaaa tccggaccga ctcccagccg tgactggaat cgaacccaga cattcgaatg
     2701 tagcgatcgc cataggtgcg gcggcagtca gcgagttcca tcatgactcc tgcggcatcc
     2761 ttcagatcga acatcggaag accccacatc tcccagtaga tattccgtgg gtggggatcg
     2821 tcggtgaatt cgatgttcac agcccagccc ttgtcgaggc aatactggac ctgtcgagaa
     2881 atctgctcgt cggtgagatc gggcaggaat gaaaaacagc cttgggtcac gcgcattatc
     2941 ttgctccttt acgttgagac gctggcggtg ggggcgtagt caggcatgtc ggtagattcg
     3001 tagttaaagg tgacgtcttt ccacacctcc agcgccgatt tcaggggggt acaggtctgc
     3061 gccgctttcg ccagaatttc cggcccttca tgcagatagt cgcggccttc gttgcgggcg
     3121 aggatcatcg cctccagcgc cacgcggttc gcggtcgcgc cggcctggat acccatggga
     3181 tgaccgatgg tgccgccacc gaattgcaac acgacatctt caccgagatg atcgagtagc
     3241 tgatgcatct ggccggcatg aatgcccccc gaagccaccg gcatcagctt gttgaggctg
     3301 gcccagtttt gatcgaagaa cacgccgtgc tcgagcttca tcggattgaa gtcctcgcgg
     3361 cagatgtcgt aatagccgcg cgtcgtcgcc ggatcgccct ccagcttgcc gaccaccgtg
     3421 ccggcatgga tgtgatcaac gcccgcgagc cgcatccact tcgttatcac gcggaacgag
     3481 acaccatggt ttcgctgccg tgtataggtc gaatgacctg cccgatgcag atgcaggatc
     3541 atgtcgttgc gacgcgccca tttcgccatc gactggatcg cggtgtagcc gatcaccaga
     3601 tcgatcatga tgacgacgga acccaattcc ttggcaaact cggcgcgctc atacatatct
     3661 tccatcgtcc ccgcggtgac gttgagatag gtgcccttga tctcgccgga cgcggcctgc
     3721 gccttgttga ccgcttccat gcagtacaga aaccgctcgc gccagtgcat gaatggttgc
     3781 gagttaatgt tctcgtcatc cttggtgaaa tcgagaccac ccttgagcgc ctcgtagaca
     3841 acgcgtccat agttccggcc cgatagtccc aatttcggct tcaccgttgc accgagcaat
     3901 ggccggccga acttgtcgag cctctcgcgc tcgaccacga tgccggtcgc cggcccctga
     3961 aacgtcttca cataggcaac cggaaaccgc atgtcctcaa gccgcagcgc tttcagcggt
     4021 ttaaagccga acacattacc gatgaccgaa gccgagaggt tcgatatcga tccgttctcg
     4081 aacaaatcga gatcataagc gatataggca aaatactgcc ccggcgagtt cggcaccgga
     4141 tcgacgcggt agcatttcgc gcgatacttt tccgcagcgg tcagcctgtc ggtccacact
     4201 actgtccatg tcgctgttga agattcaccg gcgactgccg cggatgcctc gatcggatcg
     4261 accccgtcct gcggcgtcac ccggaacagc gcgatgatgt cggtgtcctt tggcgtatag
     4321 tcgggctccc agtagcccat cttcttgtat tccatgacgc cggacttgta gcgttctttc
     4381 ccatgtaacg taacggaatg ctcgttcatt tcattctcct cacagattga ccggcgttca
     4441 tatccggatg gaagtttcct ggttctactc ggcagccgct gccattgttc cgatgcgcgg
     4501 atcaagagcg cccgcgcgat agcgcttcgc catttccgct agcggaatca ccttgatctt
     4561 cgcggcattt ccagcggtgc cgaattgctc gaaccgttcg cggcagagat cgcgcatagc
     4621 atccatcgcc ggtttcagaa atttacgcgg atcgaattcg ctcttggttt gcgccgcgac
     4681 ttttcgaaac gccgctgtca tcgccaggcg acagtcggtg tcgatgttga ccttgcggac
     4741 gccgtgcttg atgccgcgaa taatctcttc gacggggaca ccccatgtct gaggcatttc
     4801 gccgccgaac ttgttgaata tatcttgcag agactgtggt acggacgacg agccgtgcat
     4861 caccagatgg gtattcggaa gcctgcgatg aatctcctcg accaccttca tggcaaggat
     4921 atcgccatca ggcttgcgcg agaatttgta ggcgccgtgc gaggtcccca tggcgatcgc
     4981 taatgcatcg accttggtag cgcggacaaa atcgaccgcc tgatccggat ctgtcagcag
     5041 ttgatcgtgg ctaaccttgc cttcgacgcc gtggccgtct tcctgttcgc cgccgccatg
     5101 ttcgagcgaa ccgagcacgc ccagttcgcc ttccactgac gcaccgatcc agtgcgccat
     5161 ctcgaccaca cgccgggtga tgttaacgtt gtagtcataa tccgcggcgg tcttagcgtc
     5221 cgccttcagc gaaccgtcca tcatgaccga agtgaagccg tatttgatgg cggtcgcgca
     5281 ggtggcttcc tcgttgccgt gatcctggtg catgcagagc gggatctgcg gatacatctc
     5341 ctccagcgcg tcgatcatct tggacatcat gatgtcgttc gcgtaggagc gcgcgccgcg
     5401 cgacgcctga atgatgacag gcgcatcaac cgtggccgca gcgtccatga tcgcgagtcc
     5461 ctgctccata ttgttgatgt tgaatgcggg aacgccgtag ccattctcgg ctgcgtgatc
     5521 gagcaattgc ctcaaggtta ttcgagccat caatacctcc ttcaggtgtt ggatgtccta
     5581 aggattgcca cacccgggag atcgcgcccc tcgagctatt ctacaaacgc gctaccggcc
     5641 gtc
//
LOCUS       DATMGX010000076         2519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_221537, whole genome shotgun sequence.
ACCESSION   DATMGX010000076 DATMGX010000000
VERSION     DATMGX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221537"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /gene="tyrS"
                     /locus_tag="VNR65_01260"
     CDS             <1..1054
                     /gene="tyrS"
                     /locus_tag="VNR65_01260"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWJ17331.1"
                     /translation="RPIALIGGGTTKVGDPSGKDESRKLLTEAEIDANKARIRDTFGK
                     FLTFGEGKTDAFLVDNDEWLRDLHYIDFLREYGRHFTINRMLTFDSVKLRLEREQPLT
                     FLEFNYMLLQAYDFLELARRHNCVLQMGGSDQWGNIVNGIELARRVEGRELFALTTPL
                     LTTSSGAKMGKTVKGAVWLNADMLSPYDYWQFWRNTEDGDVGRFLRLFTELPLDEIGR
                     LEVLSGAEINEAKKVLATEATALLHGREAADASGETARQAFEEGAAAEGLPTVEISRQ
                     ELEVGIGLLTANTAAGLVSSTSEARRQIKGGAIRVNDVVATDERAVLTLKDLNEDGVI
                     KLSMGKKRHVLLRPAT"
     gene            complement(1152..1823)
                     /locus_tag="VNR65_01265"
     CDS             complement(1152..1823)
                     /locus_tag="VNR65_01265"
                     /inference="COORDINATES: protein motif:HMM:NF006601.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWJ17332.1"
                     /translation="MVYQTLRRYFHMASIGDQSYRFLFKKPPPDEVVAFDCETTGLDV
                     AKDDIITIAAVLIKGDRIIMREKFEALVRPDAIIKPDAMKIHRLREADVENQLPIRKI
                     LRDFLYFIGSRPLVGYYLEFDMKMIDKHVLPFIQMGLPNRRIEVSNLYYDRKYGNAPP
                     GTTIDLRFVSILKDLGLPNIGQHDALSDAVMTAMIYIVLRDMKKRGARIERERVRPLA
                     DAIEF"
     gene            complement(1839..>2519)
                     /locus_tag="VNR65_01270"
     CDS             complement(1839..>2519)
                     /locus_tag="VNR65_01270"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HWJ17333.1"
                     /translation="AFGFPPCPGKVMVQNPVWSQPVDSFIKQLKGWVLTPGGDSAMAL
                     GIFSDAVAIAGRRDLLAHAKTAFMDMMRGESAHLAHFAHLIDLFASSDIGMFGNLMVS
                     VGMHSGDIDVKKVGTFPIVHGVRTLAIEKGILETPTATRIKALAASNLLEQPFAQELI
                     SALHFFMELRLRSQLKARGKGNLARESIVNMNELTAADRDIFRNALRVVRQFRELVRH
                     RYNLQYFT"
BASE COUNT          540 a          696 c          771 g          512 t
ORIGIN      
        1 ccggcccatc gcgctcatcg gcggcggtac gacgaaggtc ggagatccat ctggcaagga
       61 cgaaagccgc aagcttctga ccgaagccga aatcgacgcc aacaaggcac ggatacggga
      121 tacctttggg aagttcctaa ctttcggaga gggaaagacg gatgcatttc tcgtcgacaa
      181 cgacgagtgg ctcagggacc tccattacat cgattttctg cgcgaatacg ggcggcactt
      241 tacgatcaac cggatgctga cgtttgattc ggtcaagctc aggcttgaac gcgagcagcc
      301 gctgaccttc ctcgaattca attacatgct gctgcaggct tacgattttc ttgagcttgc
      361 ccgccgccac aactgcgtgc tccagatggg cgggtcggac caatggggga acatcgtgaa
      421 cggcatcgag cttgcccgcc gcgtcgaagg ccgggagctt ttcgcgctga cgacgccgct
      481 gctgaccacg tcctcaggcg cgaagatggg caagacggtc aagggtgcgg tttggcttaa
      541 tgcggatatg ctgagcccct acgattattg gcagttctgg cgcaacacgg aggatggcga
      601 cgtcggccgc ttcctgcgct tgttcactga gttgccactg gacgagatcg gcaggctcga
      661 ggtgctgtcc ggcgccgaga tcaacgaggc caagaaggtg ctcgcaaccg aggcgacagc
      721 gctgctgcat ggccgagagg ctgcggatgc ctctggggaa accgcgcgcc aggctttcga
      781 ggaaggcgcg gcggcggaag gcctgccgac ggtcgagatt tcaaggcagg aattggaggt
      841 ggggataggc ctactgaccg ccaacacggc agcggggctg gtttcctcaa cgagcgaggc
      901 caggcgccag atcaagggcg gagcaatccg ggttaacgac gtggtggcca ccgatgagcg
      961 tgccgtgctg acgctgaagg atttgaatga ggacggcgtg atcaagcttt cgatgggcaa
     1021 gaaacgccac gttttgctgc ggccggcgac ataggacgcg ataagccgcc ctattataac
     1081 acttaggaat cgatcaactt ccgcgcttaa tcggttccga tgggtgatcc agcgcgtcgg
     1141 tgacgctgtc gttaaaactc gatcgcgtca gccaggggcc ggacgcgctc gcgctctatt
     1201 cgagcgccac gcttcttcat gtcacgcagg acgatgtaaa tcatcgcggt catgaccgca
     1261 tcgctcaagg catcgtgctg gccgatgttg ggcaggccga gatccttgag gatcgacacg
     1321 aagcgcagat cgatggtggt tcccggcggc gcgttcccgt atttgcggtc gtaatagaga
     1381 ttcgacactt cgatgcgccg attcggtagc cccatctgga tgaacggcag cacatgcttg
     1441 tcgatcatct tcatgtcgaa ctccagataa tagcccacca aaggccggga gccgataaaa
     1501 tagagaaaat cccgtaggat tttgcgaatc ggcaactggt tttcgacatc ggcctcgcgc
     1561 aatcgatgga ttttcatcgc atccggcttg atgattgcat ccggccgcac gagcgcttcg
     1621 aacttctccc gcattatgat ccgatcgccc ttgatcaaga cggcggcgat cgtgatgatg
     1681 tcgtccttcg cgacgtcgag gcccgtcgtc tcgcaatcga aggcgaccac ttcgtcgggc
     1741 ggcggctttt tgaagagaaa gcgataagac tggtccccga tcgacgccat gtggaagtat
     1801 cgccggagcg tctgatacac catcagaaat atccaagatt acgtgaagta ctggagatta
     1861 tagcgatggc gcaccagttc gcggaactgg cgtacgacgc gcaatgcgtt ccggaaaatg
     1921 tcgcggtcag ccgccgtgag ctcgttcata ttaacgatgg actctcgggc caggtttcct
     1981 ttgccccgcg ccttgagctg agaacgcaac cgcagttcca tgaagaaatg aagtgcgctg
     2041 ataagctctt gtgcaaaggg ctgttccagc aggtttgagg cggcgagcgc cttgatccta
     2101 gtggcggtcg gtgtctccag aattcctttc tcgatcgcaa gtgtcctgac accatggacg
     2161 atcggaaatg ttcccacttt cttcacgtcg atgtcgcccg agtgcatgcc gaccgatacc
     2221 atcagattgc cgaacatgcc gatgtcggaa ctcgcgaaaa ggtcgatcag atgtgcgaaa
     2281 tgggcaaggt gagcgctttc gccccgcatc atgtccatga atgccgtttt cgcgtgggcc
     2341 aggagatcgc gacgccccgc aatagcgacg gcgtcgctga aaatgcccag ggccatcgcg
     2401 ctatcgccgc cgggtgtgag gacccagcct ttgagttgct tgatgaagct atcgacgggc
     2461 tgcgaccaga caggattttg gaccatgacc tttccgggac atggcgggaa tccaaacgc
//
LOCUS       DATMGX010000077         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_221386, whole genome shotgun sequence.
ACCESSION   DATMGX010000077 DATMGX010000000
VERSION     DATMGX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221386"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VNR65_01275"
     CDS             complement(<1..477)
                     /locus_tag="VNR65_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWJ17334.1"
                     /translation="MAPAAETTLQLNSYRTGPDERGHFGLYGGRFVAETLMPLILELE
                     AAYNAAKNDPAFHAELADLHAHYSGRPSPLYFAERLTKHFGGAKVYFKRDELNHTGSH
                     KINNCLGQILLAKRMGKTRIIAETGAGQHGVAVATVAARFGLPCAIFMGKTDIERQK"
     gene            complement(488..1201)
                     /locus_tag="VNR65_01280"
     CDS             complement(488..1201)
                     /locus_tag="VNR65_01280"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002295.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWJ17335.1"
                     /translation="MAETARHPKSMTILVKICGISTEEALEAAIEAGADYAGFVFFER
                     SPRNVTRSRAAALARRAEGRIKIVALTVNATADVIKMIAGEVRPDFFQLHGDENLERI
                     GSVRSLTSAKIIKAIKVGTPCDIQGSRRYEDEADLLLFDAQASAALPGALPGGNGVSF
                     DWRWLQGGALRRDFMLSGGLCAENVLTALAESGASAVDVSSGVETSPGVKDPSLIRQF
                     IKAAKSFERAASPVSNAAG"
     gene            complement(1218..1583)
                     /locus_tag="VNR65_01285"
     CDS             complement(1218..1583)
                     /locus_tag="VNR65_01285"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HWJ17336.1"
                     /translation="MKRFFNVLILFIIGCLLVAFGVANRHPVTFVADPFIDEKMALSF
                     QAPLSLFLFVALFAGVIIGWMTAWVGQGHWRKRARDTHKEASIWKREAENLKRGLEAA
                     TPKAITPPEVRPLRSYFSR"
     gene            complement(1615..1989)
                     /gene="ihfB"
                     /locus_tag="VNR65_01290"
     CDS             complement(1615..1989)
                     /gene="ihfB"
                     /locus_tag="VNR65_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HWJ17337.1"
                     /translation="MIKSELIEKLSVTNPHLMHRDVEKIVNVIFEEITSALARGDRVE
                     LRGFGAFSIKHRPARVGRNPRTGDQVEVPEKFVPYFKTGKELRIRLNPPEAPTEKPKG
                     RKKAATTAAKAPKAAKVAKKAS"
BASE COUNT          451 a          652 c          611 g          469 t
ORIGIN      
        1 cttctggcgt tcgatgtccg tcttgcccat gaagatcgcg caagggagcc cgaatcgcgc
       61 ggcgacagtc gcaaccgcca cgccatgctg gccggcgccg gtctccgcga tgatgcgggt
      121 cttgcccatg cgtttcgcga gcaaaatctg ccccaggcag ttgttgatct tgtgcgagcc
      181 ggtgtgattg agttcatcgc gcttgaagta gactttcgcc ccgccaaaat gcttcgtgag
      241 cctttcggcg aaatagagcg ggcttggccg gcctgaatag tgggcgtgga gatcggcaag
      301 ctcagcgtga aaagccgggt cgttctttgc cgcgttgtag gctgcctcca gttcgaggat
      361 caacggcatc agcgtttccg ccacaaaccg cccgccataa aggccgaaat gccctctttc
      421 gtcgggaccg gtccgatagc tattcaattg caatgtcgtt tcggcggcag gggccatgag
      481 gtgatcctta tcccgcggcg tttgagacgg ggctggcggc ccgctcgaat gactttgcgg
      541 cttttataaa ctggcggatg agcgaagggt cttttacgcc aggagacgtt tccacgccag
      601 aggagacatc gaccgccgag gcgccgctct ctgcgagagc tgtgagaaca ttctccgcgc
      661 aaagtccgcc cgacagcatg aaatcgcgcc gaagtgcgcc tccctgaagc cagcgccagt
      721 cgaaagacac gccgtttccg cccggaagcg cgccgggcaa agccgcgctc gcctgcgcat
      781 cgaaaagcag aagatccgcc tcgtcctcat agcggcggct gccttggata tcacaggggg
      841 tgccaacctt gatcgccttg atgatcttcg ctgaggtcag gcttcgaact gacccaattc
      901 gctccaggtt ttcgtcgcca tgaagctgaa aaaaatcggg ccgaacctcg ccagcgatca
      961 tcttaatcac gtcggccgtg gcgttcactg tcagcgccac aatcttgatg cgaccctccg
     1021 cacgcctcgc cagagcagca gcgcgactcc gcgtaacatt ccgcgggctt cgttcgaaga
     1081 aaacgaaacc ggcgtaatcc gcacccgctt cgatcgcggc ttccaaagcc tcttcggtcg
     1141 agatgccgca aatttttacg agaattgtca tgcttttcgg atggcgggca gtttccgcca
     1201 tcgacgtcct cggaaggtca gcgcgagaaa taggagcgga gcggccgcac ctcgggtggc
     1261 gtaatagcct tgggagtagc tgcctcgagg ccccgcttca ggttttccgc ttcgcgcttc
     1321 cagatcgaag cttccttatg cgtatcccgc gccctcttgc gccaatggcc ctgcccaacc
     1381 caagcagtca tccatccaat gatcacgccg gcaaacaagg cgacgaagag gaacaaggac
     1441 agcggcgcct ggaacgacag cgccatcttt tcatcaatga acggatcggc gacaaatgtc
     1501 acggggtggc gatttgcgac cccgaaggca accagcaggc agccaatgat aaacagaatg
     1561 aggacattga agaaacgttt catgggtgcc tcggcaaaaa gcctgtcgcc agaactagga
     1621 tgcttttttc gcaactttcg ctgcttttgg tgctttcgct gctgttgttg ctgctttctt
     1681 acggcctttc ggcttctccg tcggagcttc gggcggattg agccggatac ggagttcctt
     1741 gccggttttg aagtacggca cgaacttctc tggaacctct acctggtcgc ctgtgcgcgg
     1801 atttcgtcca accctagcgg gccggtgttt gatcgagaat gcgccaaagc cacgcagttc
     1861 gacgcggtcc ccacgtgcga gcgcgcttgt gatctcctcg aagatcacat tcacaatctt
     1921 ctcgacgtcc ctatgcatca aatgaggatt cgtaaccgaa agcttctcaa tgagctctga
     1981 tttaatcatt gctttatctc gcttttcttc attgagggtg ccaaatcgag aacaggccgt
     2041 caagcggccg atccttcagt gattccagcc cttccagcac gctggcatcg atgagagagc
     2101 caaggccgag ctttcctgcg agacccgtca gagaagacga aaaaaagccg gacacggaac
     2161 ttttcggctt ccagtccttg acc
//
LOCUS       DATMGX010000078         2207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_221257, whole genome shotgun sequence.
ACCESSION   DATMGX010000078 DATMGX010000000
VERSION     DATMGX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221257"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VNR65_01295"
     CDS             complement(<1..259)
                     /locus_tag="VNR65_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17338.1"
                     /translation="MTARYIGPSLWVVFASVLGLISYLLLSACDFGRPLFGFRYCQSQ
                     VSFPHTASRYEQERERSLKRHLHEAELRTAQLPVCIQKPQPE"
     gene            425..2068
                     /locus_tag="VNR65_01300"
     CDS             425..2068
                     /locus_tag="VNR65_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HWJ17339.1"
                     /translation="MLTLRALLLSAFAAILWPVSSNAEELTDICLKSGIQVRARIETR
                     EGRFILYIQGSSTPSDMTGQIHSVGVPDDPVCKQEGDKRKDNPISQTTKFGIRGSNTI
                     GERLMPMLIEAYANKNFGAPPAVRIIEPEHQEMEIKRPGAAQPDVLIDFWSKGSGTAP
                     PALKDHLADIGMMSRRMKDEEAAAIKAAQNVNPLEPESGGEHVVALDGLAVIVNPGNP
                     VKKLSLDTVARIFSCEITNWRDVKGKNANNEDVSGPDAPISVHARDNKSGTFDTFKAV
                     VLEERKQKICDSATRYESSEALSAAVSKDLNAIGFIGFPFINRNVPLRILSACGLDSE
                     PESFQVKMETYPLARRLYLYTLGTPSERLVKDLVAFSLSDEAQKTVKEAGFIDQLVDM
                     PQQESQQRWLRTLEDNPKYGLADDKEAPRPAARQFSRLASGMQRTSIVFRFARGESVL
                     DTLARQNVGRLARYLGQPGQSGRRVLLVGFADSDGGWASNYALAVARAQTVLKELRGS
                     GQSMPQIQVYSMSYAAPVACNDSDKGRSLNRRVEVWISN"
BASE COUNT          534 a          609 c          622 g          442 t
ORIGIN      
        1 tttctggctg tggcttctgg atgcaaacgg gcaactgcgc cgtgcgaagc tccgcctcgt
       61 gcaggtgccg cttcaaggat cgctccctct cctgttcata acggctcgct gtgtgaggaa
      121 agctgacctg gctctggcaa tatcggaagc cgaaaagcgg gcggccgaaa tcacaggcgc
      181 tcaggagcag ataggaaatg aggcccaaca ccgaggcaaa aaccacccaa agcgacggcc
      241 caatataacg tgcggtcaaa gtccttcccc gttctcattc agggatggca atacgaatac
      301 aaattcctac agccggcttc gcttgtcgag caaagaaaga ctccccattg tcataaaact
      361 aaaatatgca ggcagtatga acttgaatca tttagagatt tttcaaatca gcgagtcgag
      421 aataatgctt accctgcgcg cgctcctgct ttcggctttc gcagccatcc tatggcccgt
      481 ttcgagtaac gccgaggaac tgacggacat ctgtctcaag agcgggattc aggtccgcgc
      541 ccggatagag acccgcgaag gaagatttat tctttatatt caggggtcct cgacgccgtc
      601 agacatgacg ggtcaaattc acagcgtggg cgttccggat gatccggtct gcaaacaaga
      661 aggcgataaa agaaaagata atccaatctc ccagaccacg aaattcggca ttcgcgggtc
      721 caacacaatc ggagaacgtc ttatgccgat gttgatcgag gcctatgcga acaaaaactt
      781 cggcgcgccg cctgcggtca ggatcataga accggaacat caggaaatgg agattaaaag
      841 accgggcgcg gctcagcccg atgtcctgat agatttctgg tcgaaggggt cgggcaccgc
      901 cccacccgcg ctcaaagacc atctcgccga cattgggatg atgtcccggc ggatgaagga
      961 tgaggaggct gctgcgatca aggccgctca aaacgtcaat cccctggaac cggaaagcgg
     1021 cggcgagcat gttgttgcgc tggatggtct cgccgtcatc gtcaatcccg gcaatccggt
     1081 caagaagctc tcgctcgaca cggttgcgcg catcttctcc tgcgagatca ccaactggcg
     1141 ggacgttaaa ggcaagaatg ccaataacga agacgtcagc gggccagatg cccccataag
     1201 cgtgcatgct cgcgataaca agtcggggac gttcgacaca ttcaaggcgg ttgtgctgga
     1261 ggagcggaaa cagaaaatct gtgattcggc aacccggtat gaatcgagcg aagcattatc
     1321 ggccgcggtc tcgaaggatt tgaacgcaat cggctttatc ggttttccct ttatcaacag
     1381 gaatgtgccc cttcggatcc tctcggcatg cgggcttgat agcgaaccgg aaagcttcca
     1441 ggtgaagatg gagacctatc ctctcgcacg gcgcctctat ttgtacacgc tcgggacgcc
     1501 gtccgagcgg cttgtcaagg atctggttgc cttctctctt tcagatgaag ctcagaaaac
     1561 cgttaaggag gccggcttca tcgatcaatt ggttgatatg ccacagcagg aatctcaaca
     1621 acgctggctc aggacgctcg aggacaaccc aaagtacgga cttgcggacg acaaagaggc
     1681 accccgcccc gcagcgcgtc agttttcaag acttgcctcg ggaatgcagc gcacgagcat
     1741 cgtctttcgt tttgcgcgcg gcgagtcagt gcttgatact cttgcccggc aaaacgtcgg
     1801 ccgccttgca cgttacttgg gtcagccagg gcagtcgggc cgcagagtgc ttctggttgg
     1861 cttcgctgac tcggatggcg gctgggcttc caattacgct ctcgcagtag cgcgtgccca
     1921 gactgtcttg aaggagctca gaggctccgg ccagagcatg ccgcaaatcc aggtttacag
     1981 catgtcgtat gctgcccccg tcgcctgcaa cgatagcgac aaaggaaggt cgctcaaccg
     2041 ccgggtagaa gtctggattt caaactaata ctgggaagcg aagccagcgg catcgaggcc
     2101 ccagggagcc gccggcaaag tcctctgagc tttgcgggat aaggtgcaga tgtgcactgg
     2161 gccagcccta ttagcccagg ccaatgatac gaaaaaaaag tgtgccg
//
LOCUS       DATMGX010000079         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_153398, whole genome shotgun sequence.
ACCESSION   DATMGX010000079 DATMGX010000000
VERSION     DATMGX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153398"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VNR65_01305"
     CDS             complement(<1..254)
                     /locus_tag="VNR65_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17340.1"
                     /translation="MVRIGFAVRVAFCTSLLLSTETQAQEAGQQSQPQGQAQQAQGAQ
                     KPQTLPPVVVKNARATKAKRREFIKKKTPEPAPAPTHEAPS"
     regulatory      complement(362..538)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            735..1145
                     /locus_tag="VNR65_01310"
     CDS             735..1145
                     /locus_tag="VNR65_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17341.1"
                     /translation="MISNGRLRWKLLGGLLAAVLFDTLLQITWKSAVLEAPSDPSPMA
                     AVSSLLSNPALLGIITIMAFQFFNWLMVLGEADLSYAKPVASLSYACVPIVSVLMLHE
                     AVDGFEIAGVVFVIAGVWFIAQTKPMTEDTTTLP"
     gene            1142..1474
                     /locus_tag="VNR65_01315"
     CDS             1142..1474
                     /locus_tag="VNR65_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWJ17342.1"
                     /translation="MTGTTFGILLVVLCAFLEGLGQVFLKKSVLTKVRWFMWISAGVV
                     VLALQALIYTEALKLLDVSVAFTILSLNLIAITVLSHWILGERVTRTRWIGVGLIFVG
                     VALVMAGT"
     gene            1582..1779
                     /locus_tag="VNR65_01320"
     CDS             1582..1779
                     /locus_tag="VNR65_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17343.1"
                     /translation="MCAAIDLICAQTLTADNVRARTRIPDYSNRTGLPKTAAQMRGAA
                     AKDFKIPADMRAAPVSVGTFP"
     gene            2053..>3101
                     /locus_tag="VNR65_01325"
     CDS             2053..>3101
                     /locus_tag="VNR65_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ17344.1"
                     /translation="MIQGLAVPRSYDLFHQLSSQWTDKHKVAIETGAGKHYHYSDILA
                     ATSRFAHALVAGGVKPGDRVAAQVEKSPEALFLFLACLRAGAAYLPLNTAYTEPELDY
                     FLRDSEPSVFVCRPGMSEAGALCERAGVPTLVTLGTDEDGTLPAHASGQPDVFEDVPR
                     SADDLAAVLYTSGTTGRSKGAMLSHGNLTSNAAALAQAWRFTSNDVLLHALPIYHTHG
                     LFVATNVVLISGGSMLYLPKFDPNEIFRLLPRATTMMGVPTFYVRLLDDARLTREATA
                     QMRLFVSGSAPLLPDTHRGFLEKTGHSILERYGMTETGMNTSNPYEGERVPGTVGLPL
                     PGVEVRIANPETGETL"
BASE COUNT          566 a          894 c          871 g          770 t
ORIGIN      
        1 gatggcgcct catgggtggg tgctggcgca ggctcgggcg tttttttctt tataaattcg
       61 cgccgcttgg ctttggtagc acgcgcattt tttacgacca ccggaggcag cgtctgcggc
      121 ttctgcgcgc cttgtgcctg ctgagcctgc ccttgaggtt ggctctgctg accggcttct
      181 tgagcctgtg tttccgtgct caggagtaaa cttgtacaaa acgccaccct tacggcaaaa
      241 ccaatgcgaa ccatagcagg ccccgtgaca acgtgatcgt caccacgaac ccaagctaag
      301 gaagcggcct cgctgaagcc ggaccctcat ctccatcggt tcaccccgtc caatgcgttc
      361 gcgcgtgacc acggctgatg gcaggtctcc tggctcacgg gtctcagctt cgcgcgcctt
      421 cccggtttcc cagtggctta aagcgcttcg ctcaccgctt acagttgcgg gggcagccgc
      481 ggtttcacac cgcgttccct tttatcctcc tcggaggacc atcgtgccga accttaccca
      541 gcgcttgatt gattcggcaa cgtcaccgct ctcgaatttt tgacttctcg tgagggctga
      601 ggcggatgtg acacgcattt tgcatggtac gtctttttgt tggcgcagaa tcgtgcccaa
      661 gcatttagaa tactatgaac cgatggggca gacgccggat gactatggct caatgaacat
      721 ggaggccaca gccgatgatc tcaaatgggc gcttgagatg gaaactcctg ggcgggcttc
      781 tcgcggcagt cctctttgat acgcttcttc agatcacctg gaaatccgcc gttctcgaag
      841 cgccttccga tccttcgccc atggcggcgg tgagctctct cttgagcaat ccggcccttc
      901 tcggaatcat cacgatcatg gcctttcagt tcttcaattg gctgatggtt cttggcgaag
      961 ccgatctcag ctatgcgaag cctgttgcgt ccttaagcta tgcatgcgtt cccattgtat
     1021 cggttttgat gctgcacgag gctgtcgacg gttttgagat tgcgggtgtt gttttcgtta
     1081 tcgctggcgt atggttcatc gcccagacga agcccatgac agaggacacg accacacttc
     1141 catgactgga accaccttcg gcattttgct cgtcgtcctc tgcgccttcc tggaagggct
     1201 cgggcaggtt tttctgaaga aatcggtgct caccaaagtc cgctggttca tgtggatttc
     1261 ggccggggtg gttgtgcttg ctttgcaagc cttgatctac accgaggctt tgaagctgct
     1321 ggacgtcagc gtggctttta cgatcctgag ccttaacctg atcgctataa cggttctttc
     1381 ccattggatt ttgggggaac gggtgacaag aacgcgatgg atcggcgtcg ggctcatttt
     1441 tgtcggtgtc gccctggtta tggccgggac ctgaacgctg ccgttaagca caaacgctgg
     1501 cttatccact attgctgaaa tcgtttgggc tgtttggata cacgcatctg acggacggct
     1561 atcctggcgg ccaggctgag tatgtgcgct gcgatcgacc tgatctgcgc gcagaccttg
     1621 accgctgaca acgtgcgcgc caggacacgc atccccgatt acagcaaccg gactggtttg
     1681 ccgaagactg cggcgcagat gcgcggtgcg gcggcgaaag acttcaaaat accggctgat
     1741 atgcgcgctg cgccggtcag cgtggggacg tttccataat atccagcaag aattgtatac
     1801 gtttctaaca gtttagtgca caattgtcgg ctaggtcaca agcgttagcc tagccatttt
     1861 caaagcgttc cacaagatcg aatttcctga acttaacgcg cttccgatat ttcgtgctga
     1921 cgcggggcct atcttaccat attttgtgtt ttctcttctg cagccctcat gtcattccat
     1981 gcatgcgccc aggttcttca aatacgattg tcttggcaac gcttccgtgg tcttggtatt
     2041 ctgaatattc acgtgattca gggtttggcc gtgccaagat cttatgatct ttttcatcag
     2101 ttaagttcgc aatggacgga caagcacaag gttgcgatcg agacaggtgc aggaaagcac
     2161 tatcattata gcgatatctt ggccgccacg tcgcgcttcg cccatgcgct tgtggctggg
     2221 ggggtgaagc caggcgatcg cgtcgccgct caggttgaga aatcgcctga ggccctgttc
     2281 ctgtttctgg cgtgccttcg cgccggggct gcgtatctcc cgcttaatac agcctatacc
     2341 gagcctgaac tcgattactt tctgcgcgac tcggaaccct ccgtttttgt ctgccgccct
     2401 ggcatgagtg aagccggggc actgtgcgag agggcagggg tgccgaccct agtgacgctc
     2461 gggacggacg aagatggcac gcttccggcg catgcctccg ggcagccgga tgtcttcgaa
     2521 gatgtgccgc gatcggccga cgatcttgct gcggtacttt atacatctgg cacgacaggc
     2581 cgatctaagg gcgcgatgct gtcgcacggc aacctcacgt ccaatgcggc ggcgctggct
     2641 caggcatggc ggttcacatc caatgatgtg ctgctgcatg cccttccgat ctaccatacc
     2701 cacggcctgt ttgtggcgac caacgtggtg ctgatctcgg gaggctccat gttgtatctc
     2761 ccgaagttcg atccgaacga gattttccgg ctcctgccgc gcgccaccac tatgatgggg
     2821 gtgccgacct tttatgtccg cctcctcgac gacgctcgcc ttacacgcga ggcgacagcg
     2881 caaatgcgcc tcttcgtttc aggctcggcg ccccttctgc cggataccca ccgcggtttt
     2941 cttgaaaaga ccggtcactc cattcttgaa cgctacggca tgacggaaac cggcatgaac
     3001 acctcgaacc cttacgaggg tgagcgcgtg ccgggcacgg tcgggctgcc cttgccgggc
     3061 gtcgaggttc gaatcgccaa ccccgagacg ggtgagacgc t
//
LOCUS       DATMGX010000080         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_278281, whole genome shotgun sequence.
ACCESSION   DATMGX010000080 DATMGX010000000
VERSION     DATMGX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278281"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VNR65_01330"
     CDS             complement(<1..702)
                     /locus_tag="VNR65_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWJ17345.1"
                     /translation="MKSPGRTFTLRVLFSCAILALSFYSAIPIARGEDAGRAPDSGKR
                     VALVIGNGAYPVSPVATALADARAIAELLKDGGFDVVYAQDARHADLNSAIEQFSRKL
                     ERGATAVVYFAGHAIQNQDRNFLVPVDATIASDADLRSSTVDVDLILDPLIVARPRGS
                     VVILDAARKNPWQQKVSARAKGLANVTPIEGITQAYPAAPGQIVEDNKGPVALFASEF
                     IKAAKAPDRTFKEAFN"
     gene            952..>2032
                     /locus_tag="VNR65_01335"
     CDS             952..>2032
                     /locus_tag="VNR65_01335"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWJ17346.1"
                     /translation="MKVVCAVCLTLYSIFLTLVFLPEAHADGRVALVIGNAAYSNAPP
                     LRNSRNDADDMSAQLRRLGFDVVDGRDLDNRGMRAALARFAQKLTGTDAALFYYSGHG
                     LQIDGQNYLAPVDAQVDGDSVMAFELVKLDDVIEALGYTAGTRLLVLDACRSNPFAKA
                     LAQRSGSRGGEITRGLARIERTQGMLIAYATQPNAVAADGTGRNSPFTSALVREMQEP
                     GVEVGALFRHVAVKVNRDTHGQQTPELSVSLLGDFYLNLQESDLDAFRKLGPSTGVDA
                     WKAFIEKYPSSSLVDFARARIDAIENVNARDRLVNAYEEKQRQLRLDLEQAEAGFRQA
                     REELARLRQQDEPKQAEERLSANAPA"
BASE COUNT          430 a          582 c          582 g          438 t
ORIGIN      
        1 attaaatgcc tctttgaagg ttcgatccgg agctttcgcc gctttgatga actcgctcgc
       61 aaacaaggca acggggccct tgttgtcttc gacaatttgc ccgggtgcgg cagggtaggc
      121 ctgggtgatc ccctcgatcg gagtcacatt ggcaagacct tttgcccttg ccgagacctt
      181 ttgctgccat ggattctttc tggccgcgtc gagaatgacg accgaacccc ttggacgggc
      241 aacgatcaac ggatcaagga tgaggtcgac gtccactgtg ctggaacgaa gatcggcgtc
      301 tgatgcaatt gttgcatcga ccggtaccag gaaattgcgg tcttggttct ggatggcatg
      361 accggcgaaa tagacgacgg cggtggctcc tcgctccagt tttcgactga actgctcgat
      421 tgccgaattc aaatcggcat gccgcgcatc ctgggcatag actacatcaa agccaccgtc
      481 cttcagcagt tcggcgatcg ccctcgcatc ggcgagcgcc gtagcaaccg gtgataccgg
      541 ataagctcca tttccgatga cgagcgcgac tcgcttgccc gaatccggcg ctctccccgc
      601 gtcctcaccc cgcgcgatcg gtatcgctga ataaaatgat agagcgagta tcgcgcacga
      661 aaagagcact ctcagggtga aggttctgcc gggcgatttc ataccttttc gaccctatgc
      721 tggataagcg cagttctgcc gcaatgcaca ggtcctccgc gcatccgctc ccatccgcag
      781 gtaaaccgaa acaaagcaaa cgaactgcct gccagccaaa atgtcgcatc aaagctaaca
      841 cgttgaccgc gcagaaaaca agaagttgcg gtcaccgcat atttctctta atgtcaaatc
      901 ttgggcggcc gtgtgggcgc cgttgtgtat ttaattggcg gaattccgtc cgtgaaagtg
      961 gtttgcgcgg tttgccttac gctatattcc attttcctga ctctggtttt tctgccggag
     1021 gcgcacgccg acgggcgcgt cgcactggtg attggaaacg cggcgtattc caatgcgccc
     1081 ccgctccgca acagtcgcaa tgacgctgat gatatgtccg cgcagttgcg gcggctcggc
     1141 tttgatgtcg tcgatggccg cgaccttgat aatcgcggca tgcgcgccgc gctcgcgcgc
     1201 tttgcacaga aactgacggg aaccgacgcg gccctgtttt actattcggg gcatggactt
     1261 cagatcgacg gtcagaacta tctcgctccc gtcgatgccc aggtcgacgg cgattcagtc
     1321 atggcattcg agttggtcaa gcttgacgac gtcatcgagg cgctcggata caccgctggc
     1381 acccggctcc tggttctcga cgcctgccgc agcaatccgt tcgccaaggc gctggcgcag
     1441 cgtagcggct ctcgcggcgg cgaaattact cgaggtctcg cacggatcga gcgaacccag
     1501 ggcatgttga tcgcctatgc cacgcaaccc aatgccgttg cagccgatgg cacaggtcgc
     1561 aacagtccgt tcacaagtgc actggttcgg gagatgcagg agcctggcgt ggaagtcggc
     1621 gcgctgtttc gacatgtagc cgtgaaagtt aaccgcgaca cccacgggca gcaaactccc
     1681 gaactttcgg tttcgctgct tggcgacttc tatttgaacc ttcaggagag cgaccttgac
     1741 gccttcagga agttagggcc gtccacaggt gtggacgcgt ggaaagcgtt catcgagaaa
     1801 tatccttcga gcagtctggt cgattttgct cgcgctcgaa ttgatgccat cgaaaatgtg
     1861 aatgcacgag atcgactcgt caatgcgtac gaagagaaac agcgacagct tcggcttgat
     1921 ttggaacagg ccgaagcagg ttttcgacaa gcgcgggagg aactggctcg attgcggcag
     1981 caagacgaac ccaagcaagc cgaagagcgt ttgagtgcaa atgcacccgc tg
//
LOCUS       DATMGX010000081         3604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_367888, whole genome shotgun sequence.
ACCESSION   DATMGX010000081 DATMGX010000000
VERSION     DATMGX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367888"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VNR65_01340"
     CDS             complement(<1..210)
                     /locus_tag="VNR65_01340"
                     /inference="COORDINATES: protein motif:HMM:NF037356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17347.1"
                     /translation="MSAEPRVPLPPISAQKFSTVCQFCIVGCGYTVYKWPVGKDGGPN
                     PADNAARINFNRPQPPLTGEWISPLM"
     gene            complement(244..783)
                     /locus_tag="VNR65_01345"
     CDS             complement(244..783)
                     /locus_tag="VNR65_01345"
                     /EC_number="1.20.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050611 - arsenate reductase (azurin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (azurin) small subunit"
                     /protein_id="HWJ17348.1"
                     /translation="MTTKPGCACAHSPCDPARRDFIRLGAGTLSAGVAYAIAPVTAHG
                     AEGKTAAPVVPTVNVTPLDAVKENSVVPFAYPDAESPAVLLRLPTPAQGGIGPGSSIV
                     AFSVLCTHKGCPVSYRAEHKLLICPCHWSTFDPAKGGQMVIGQGSQALPQIELRVKDN
                     VIQAVGITGLIYGRHTNIL"
     gene            964..1308
                     /locus_tag="VNR65_01350"
     CDS             964..1308
                     /locus_tag="VNR65_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWJ17349.1"
                     /translation="MKEKDVVRCLAALAQPIRLQVFRALVVRGRTGLTPSAMSEGLDI
                     PANTLSFHLKELANAGLVTQERLSRNIIYRASFDDMNALLGFLTRNCCQGAGCAAVEP
                     RDAGTRKGRQPA"
     gene            1472..1966
                     /locus_tag="VNR65_01355"
     CDS             1472..1966
                     /locus_tag="VNR65_01355"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWJ17350.1"
                     /translation="MSESLYNVLFICTGNSARSILAEGLLNDLGKGRFQAFSAGSHPK
                     GEVHPLALATLRRLKIPADGYRSKDWEEFAGPSAPRLDFVFTVCDNAAGEVCPVWPGQ
                     PMTAHWGVPDPASVEGDLEQQSKAFFDTAVILRRRIELLVHLPLRSLDELSIRREIRE
                     IGRQ"
     gene            complement(1994..2476)
                     /locus_tag="VNR65_01360"
     CDS             complement(1994..2476)
                     /locus_tag="VNR65_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17351.1"
                     /translation="MLRQRRSRKPLYFPLGVLSRARVLPDRSAEQRIQDFLKWSGLLS
                     LLSLNLVGFMFGLKWLPVVAALLVAWCYFGIRAHLSGYAFSSYSHPLVQSYAAAPDPP
                     SKLGLWFTLAACIALVACGSYAISQAEVAGDVLMAGLSVLVFGACAGMVAYVLRAHYP
                     "
     gene            complement(2617..3177)
                     /locus_tag="VNR65_01365"
     CDS             complement(2617..3177)
                     /locus_tag="VNR65_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWJ17352.1"
                     /translation="MQYGFYFDHERCVKCHACEIACKTWNEVEIGPRWREVVKIETGT
                     YPDVHAMNVSMACMHCGDAPCKTACPVSAISKSAENGIVTVDPNKCIGCGFCAWACPF
                     GAPQLSAAAGKMEKCNFCQTPGKVRPLGMPRACEEICPTGAIQSGPMTALATQGRMKA
                     AGKAAGMGPGLPSIVLDAQQRFGVSS"
     gene            complement(3227..>3604)
                     /locus_tag="VNR65_01370"
     CDS             complement(3227..>3604)
                     /locus_tag="VNR65_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWJ17353.1"
                     /translation="HLTHSKLSMHCNIVTQNNPYLMEICGENWVEINRVDAQKYGIQN
                     DTYAIVESPKDSIRIKAKVVEGLVPGCVSVRHGHGFGHWAMGSIAKGRGAHSNNLMES
                     HSSPITGANCYNECKVRVRPAPN"
BASE COUNT          680 a         1088 c         1153 g          683 t
ORIGIN      
        1 catgaggggc gatatccact cgccggtaag cggcggttgc gggcgattga agttgattcg
       61 agccgcgttg tccgccggat tcgggccgcc gtctttgccc accggccact tgtagaccgt
      121 gtagccgcag cccacgatgc aaaactggca aaccgtcgaa aacttttgcg cgctgatcgg
      181 cggaagcggg accctcggct ctgcgctcat gaccacgctt cggcaacagc ccggggaagc
      241 ggatcagagg atgttcgtat ggcggccata gatcagccca gtgatgccga cggcctggat
      301 cacgttgtct ttgacgcgaa gctcgatctg gggcaaggcc tggcttccct ggccgatcac
      361 catctggccg cccttggctg gatcgaacgt cgaccaatgg cagggacaga tcagcagctt
      421 gtgctcggca cggtagctga ccgggcatcc cttgtgcgta cagagcaccg agaaggcgac
      481 gatgctggaa cccggcccga ttcctccttg cgccggggtc ggcaggcgca gcaagaccgc
      541 cggtgactcg gcgtcgggat aggcgaacgg gacgaccgaa ttctccttga ctgcgtcgag
      601 aggagtcacg ttcaccgtcg gcacaaccgg cgcagctgtc ttgccttcgg ctccgtgcgc
      661 ggtgaccggc gcgatcgcgt atgcgacacc ggcagatagc gtgcctgctc cgagcctgat
      721 gaaatcccgc cgagccgggt cgcaaggaga gtgcgcgcag gcgcagccgg gcttggttgt
      781 catgcctctg gagccttgtg tctgaagccg ggaatcattc gagtatgcat tgatcgtaga
      841 acagttgttg catcacgcgc aagcgacagg gggcttcggc gcacaacgtg cgggtggccc
      901 gaccgcgatg gagcagcgct cgcggctcac gcgtcacaaa agtgattcga ctaaacttga
      961 aaggtgaaag aaaaagatgt ggtcaggtgc cttgcggcgt tggcccagcc gatccggctg
     1021 caagtctttc gcgccctggt cgttcggggc cggacaggac tgacgccaag cgccatgtcc
     1081 gaggggctcg acatccccgc aaataccttg tcgttccatc tcaaggaatt ggcaaacgca
     1141 ggcttggtca cgcaggagcg gctcagtcga aacatcatct accgcgcttc ctttgatgac
     1201 atgaacgcct tgctcggctt tctcaccagg aactgctgtc agggggccgg ctgtgctgca
     1261 gtcgagcccc gtgatgccgg tacccgcaag gggcggcagc cggcttgaac cttgcagccg
     1321 gagtcgaccg cgatgaacgc cggtcgtacg cggaggttcg gcctatgcag ctccggctcc
     1381 ggaaatatga atcaacatgg cgatgtaacg cggctgtcat gtgaggccct gaaactgacc
     1441 ggcggtcttc tttgcagtgc ctcccctggc tatgtccgaa tcgctctaca acgtcctgtt
     1501 catctgcacc ggaaactcgg cgcgttccat ccttgccgaa ggactgttga atgacctggg
     1561 caagggacga ttccaggcgt tttcggcagg aagccatccc aagggcgaag tccatcctct
     1621 ggccctagcc accttgaggc gattgaagat tccggccgat gggtatcgca gcaaagattg
     1681 ggaagagttc gcgggcccga gcgctcctcg tctcgatttc gtcttcaccg tgtgcgacaa
     1741 cgcggcaggc gaggtttgcc cggtctggcc gggccagccc atgacagcgc actggggagt
     1801 tcccgacccc gccagtgtcg agggagacct ggagcagcaa tccaaggcgt tcttcgatac
     1861 ggcggtgatt ctgcggcgcc gcatcgagtt actggtccac ctgccattgc gcagccttga
     1921 cgagctctcg attcgacggg aaatacgaga gatcggacgt caatagacga tctgactggc
     1981 tcacactgac cgcctagggg tagtgagctc gcagcacgta ggccaccatg cctgcgcaag
     2041 caccgaacac caggacggac aggccggcca tcaacacgtc gccggccacc tctgcctggc
     2101 tgatcgcgta cgaaccgcaa gcgacgagcg cgatgcaggc cgcaagcgtg aaccacaggc
     2161 ccagtttgct tggaggatcg ggagccgcgg cgtagctctg cacgagggga tgactgtagc
     2221 tgctgaaggc atagccggag aggtgggcgc ggatgccgaa atagcaccat gcgaccagaa
     2281 gggccgcgac gaccggcaac cacttcaagc cgaacatgaa tcccacgaga ttgaggctga
     2341 gcaggctcaa cagaccggac cacttcagga aatcctgaat gcgctgttcg gcggaccgat
     2401 cgggaagaac ccgtgccctg ctcagtacgc caagcgggaa gtaaagaggt ttccgacttc
     2461 gtctttgacg aagcactcgt tgcgcaagcg gtcaacgtat cccatgagaa ggaattccgg
     2521 ctgccgaaga agagtgcgct ctcggtgata ggaggccctc taccgtggtc gtcgcgattt
     2581 gcggaaggct gcgagtcgtc gacgaaggac ggatgcctag ctcgaaacgc cgaatcgttg
     2641 ctgggcgtcg aggacgatcg agggcagtcc tggccccatg ccggctgctt tgccggccgc
     2701 tttcatgcgc ccttgggtag ccagggcggt catgggcccc gactggatgg cacccgtcgg
     2761 acagatttcc tcgcaggcgc gcggcatccc gagggggcgg accttgcccg gtgtctggca
     2821 gaaattgcat ttctccatct tgcccgcggc tgccgacagc tgcggcgcac cgaacgggca
     2881 ggcccaggcg caaaagccgc agccgatgca cttgttggga tcgaccgtga cgatgccgtt
     2941 ctccgcgctc ttgctgattg cggaaaccgg gcaggccgtc ttgcacgggg catcgccgca
     3001 atgcatgcag gccatcgaaa cgttcatcgc gtgcacgtcc ggataggttc ccgtctcgat
     3061 cttgacgacc tcgcgccagc gcggcccgat ctccacttcg ttccaggtct tgcacgcgat
     3121 ctcgcaggcg tggcacttca cgcagcgctc gtgatcgaaa tagaagccgt attgcaagtc
     3181 gttctcccgt cgggggcgtg aagcgcggcg atcttcgcgt cgactgtcag ttcggcgcgg
     3241 ggcgaacgcg caccttgcac tcgttgtagc aattcgctcc ggtgatcgga ctgctatgcg
     3301 attccatgag gttgttcgaa tgggcgcccc ggcccttggc gatggatccc atggcccaat
     3361 ggccgaatcc gtggccatgg cgcacgctca cgcagccggg caccaggccc tcgacgacct
     3421 tggccttgat gcggatgctg tcctttggcg attcgacgat cgcatacgtg tcgttctgga
     3481 tgccgtattt ctgcgcatcg acccggttga tctcgaccca gttctcgccg cagatctcca
     3541 tgaggtacgg gttgttctgg gtgacgatgt tgcagtgcat gctgagcttg gagtgcgtga
     3601 gatg
//
LOCUS       DATMGX010000082         3079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_399022, whole genome shotgun sequence.
ACCESSION   DATMGX010000082 DATMGX010000000
VERSION     DATMGX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399022"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1494)
                     /locus_tag="VNR65_01375"
     CDS             complement(217..1494)
                     /locus_tag="VNR65_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015497490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HWJ17354.1"
                     /translation="MKKPTTLTAVVPAANIEEAWQEVGASFERFCLTAGLATLSSMME
                     EDAVQLCGPRYERKDGKAGHRWGKTKGKLGFHGGKVELERPRIRARNGKEMPLPSWEE
                     AVSNDLLGKWAMNLMLINVSTRKYGRAVRLPEGGIGAPSGSGVSKSAVSRRFVALSAA
                     RMKEWMAADLSKLDLLIIQIDGIHIKDDLMLLAAVGVDGKGGKHPLGVIEGATENATV
                     AQALLDNLIERGLDPKVCRLFVIDGAKALSKAIRKTFGRHTPIQRCQVHKARNIMERL
                     PKSLQAGVRKVLRQAWELSDAAKAEKLLRNLARRLDHDAPGVSGSILEGLDEILTVSR
                     LGLPAELRRSLACTNIIENMNGTIRRVCRNVKRWNDASMALRWTGAAMMEAAKGFRRL
                     KACKHLPALRAALAAHQIKHTINSNIEDRALAA"
     gene            1814..>3079
                     /locus_tag="VNR65_01380"
     CDS             1814..>3079
                     /locus_tag="VNR65_01380"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HWJ17355.1"
                     /translation="MTQAGDGERGSATEQFGMIEHAGRKAILAELHARPFLPVVAPCR
                     IYHFAFATSDEEARNDRKAIADLCARSGVAKPPEDAKFHHVELADWDLRWEQHTEFTT
                     YTWSTTQGAVEPFVHPDPLGAKEIAFLAPGPLVVATHLALIEGGPSHESLAALFNVQS
                     LCIIGAAKGAAQVLTDFTLDRHGFTRLLIRSDKLGEIRAGRLVQRALEIETYRTLALL
                     GLPEARRAGPALGCMEREISEINQALSEARDQRTSQDLLKRLSDLLAGSEALSTQTAF
                     RFGASRAYHAIVKNRLDLIQEAKVGSYTTISAFFSARLDPAIETCNAIETRLGRLSSQ
                     VVRAIDLMRTGITFELEQQNRDLLQDMNRRARLQMRLQGIVQGLSTAALAYYLSGLTV
                     YLAKGMKDAGWLPKDVTADTFVALALPVTI"
BASE COUNT          601 a          960 c          897 g          621 t
ORIGIN      
        1 cgtcccgctc atcgcccaaa attgacaccc tggtgaataa ttcaggttag atacttacga
       61 aggccgccct tgcgagtagg cccagggaat ttcaccccga gcaccgggtc aacgctcgtg
      121 acacaagacc ctttgcggaa ctttcctaag cgtgtttccc ggagttgccc cgggctttgt
      181 tgaaatatgc tcggcaggca tcactggttg agcagcctac gctgcgaggg ctcggtcttc
      241 aatattgctg tttatcgtgt gcttgatctg gtgagcagca agggcggccc gcagggctgg
      301 cagatgcttg caggctttca gccgccggaa gcctttggcc gcctccatca tggcagcccc
      361 ggtccagcgc agtgccatgg aggcatcgtt ccagcgtttg acgttgcggc agacgcggcg
      421 gatcgtgccg ttcatgttct cgatgatgtt cgtgcaggcc agcgagcggc gcagttcggc
      481 aggcaagccc aggcggctga cggtgaggat ttcatccagc ccttccagaa tgctgccgga
      541 aacgccaggg gcgtcgtggt ccaggcggcg ggcaagattg cggagaagct tctcggcttt
      601 tgctgcatcg ctcagttccc acgcctgccg caacaccttg cgcacgcctg cttgcagaga
      661 tttgggcaag cgctccatga tattgcgggc cttgtggact tggcagcgct ggatcggcgt
      721 gtggcgcccg aaagtcttgc ggatcgcttt ggaaagcgcc ttggcgccgt cgatgacgaa
      781 cagccggcaa accttcggat caagaccgcg ctcgatcagg ttgtccagca gggcctgcgc
      841 cacggtggcg ttctctgttg ccccctcgat cacaccgagg ggatgcttgc cgcccttgcc
      901 gtcaacgcca acagcggcca agagcatcag gtcgtccttg atgtggatgc cgtcgatctg
      961 gatgatcagc aggtccagct tggacagatc cgccgccatc cattccttca tgcgcgcggc
     1021 cgacagcgcc acgaagcgac gggacaccgc cgatttggac acacccgaac cgctgggcgc
     1081 gccgatgccg ccttccggca ggcggacggc acggccgtac ttgcgcgtcg agacgttgat
     1141 cagcatcagg ttcatcgccc acttgcccag caagtcattg gacacggcct cctcccagct
     1201 cggcagaggc atctctttcc cgttgcgagc gcggatcctg ggccgctcaa gctcgacctt
     1261 gccaccatgg aagcccaatt tccctttggt cttcccccag cgatgcccgg ccttgccgtc
     1321 cttgcgctca tagcggggac cgcagagctg gacagcatct tcttccatca tgctcgataa
     1381 cgtcgccagc cccgcagtca ggcaaaagcg ctcgaagctg gcgccaacct cctgccaagc
     1441 ctcttcgatg tttgccgcgg gaacaacggc ggtcaatgtg gtaggtttct tcatggcgtt
     1501 gctttccttt cgtggattcg acaccccgag cctaccggct caaggcgagc aacgcctgcc
     1561 tctcattttc tacattcagc ggggcaacgc ccagctcact ctgccaacgg gatgccgaca
     1621 cgaagtagcc gaagtccttg ccttcaggtg tcttttggac tgtgtacgag gcatcgcgcg
     1681 gcgaggtagc agagccgagg taacaacgtt cttcagtccc gggcaagatt ccgccattcc
     1741 cttgccccgc gataagaact aacaaacgat tggtatagac tgcgcaagcc ccagaccaga
     1801 gatggaaaga agcatgacgc aagccggaga cggagagcga gggtcggcaa ctgaacagtt
     1861 cggcatgata gagcacgcgg ggcgcaaagc tattcttgcc gaacttcacg cacgcccatt
     1921 cctgccagtt gtagcgcctt gccgaattta tcacttcgcc ttcgcaacca gtgacgaaga
     1981 ggcccgcaac gaccgaaagg caatcgccga tctttgcgcg agaagcggcg tagcgaaacc
     2041 gcccgaagac gccaaattcc atcacgttga gttagcggat tgggaccttc gctgggagca
     2101 gcacaccgag ttcacgactt acacatggtc gacgacgcag ggcgcggtgg aaccctttgt
     2161 acatcccgat cctctggggg caaaggagat cgcctttctc gcgccggggc ctctcgtggt
     2221 tgcaacgcac ctggcgctga tcgagggcgg accttctcac gaaagtctcg cggcattgtt
     2281 caatgtgcaa agcctttgca ttatcggcgc agccaaaggc gccgctcagg ttctgaccga
     2341 ttttaccctc gatcgccatg gctttacgcg cctgctcatc agatcggata agcttggcga
     2401 gataagggcg ggccgactcg ttcagcgcgc gctggagatc gaaacctatc gcacgctggc
     2461 ccttctaggc cttccggaag cccgccgcgc cggcccggct ttggggtgca tggaacggga
     2521 aatctctgag atcaatcagg ctttgagcga agcccgcgac cagcgcacca gccaggatct
     2581 tctgaaacgc ttgagcgacc tccttgcagg aagcgaggca ctttcgacgc agacggcgtt
     2641 ccgtttcggg gcgagccgcg cttatcacgc gattgtcaaa aaccgtctcg atctcatcca
     2701 ggaggcgaaa gtcggcagtt acacgaccat ttcggcgttc ttcagcgcaa ggcttgatcc
     2761 tgcgatcgaa acttgcaacg ccatagaaac gcgcctgggg cgcctctcaa gccaagttgt
     2821 gcgcgccatc gatctcatgc gcaccggcat cacattcgag cttgagcaac agaaccgcga
     2881 ccttctccag gacatgaacc gccgcgcgcg cttgcagatg cgcttgcagg gcatcgttca
     2941 gggcctttcc accgcggcgc ttgcctatta tctctctggc cttacggtct atctcgccaa
     3001 gggaatgaag gatgctggat ggctgccgaa ggacgtgacg gcggatacat ttgtagccct
     3061 ggcgctcccc gttacgatt
//
LOCUS       DATMGX010000083         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_17466, whole genome shotgun sequence.
ACCESSION   DATMGX010000083 DATMGX010000000
VERSION     DATMGX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17466"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="VNR65_01385"
     CDS             complement(<1..1769)
                     /locus_tag="VNR65_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWJ17356.1"
                     /translation="MSQVTGIRHHLVAILAADAAGYSRLMVDDEHATVLALDRARDVF
                     RKAAEADHGRIVDTAGDSVLVVFETAAGAVRAALRIQQALKESSGEEASDRRLCFRIG
                     VHLGDVLEKDDGSVYGEGVNIAARLQELALPGGVVVSDSVRSSIRSRLPATFLDLGDK
                     TVKNMAEPVHAFRVTAGGGPNRPGRISSIGETEGMFAGAAHAWRRVPLGIRRWLLATM
                     CALIVGVVGWMRLQPPVATSRSIAVLPFVDMSENHDQEYFSDGLSEELIDHLTNAPGL
                     KVIARTSSFQFKGKNEDMRGIAQKLGVANLLEGSVRKSGDDVRITVQLIRATDGTHIW
                     SQIYDRNFSDIFKIQDEIARTVADSLRVALESNDSHEGRPANTQAYNLLLEGDFFARR
                     NTKPDTEHAIGLYKDAIALDQNYARAWARLADAYGALGWFGLSNVDGQREAIAQALRA
                     DPESPDAYGAKAYLLKYHDWDWSGAEAALRRANELAPNPRLDEDLAEIGWMFGRLDDS
                     IAAHRRRLVRDPLSANAYWGLGLDLFIEGRYKDAESAFRKVSELNTSYASAEAFLALV
                     LLYQGRGPEALLAVNQEPDASWKA"
BASE COUNT          414 a          719 c          621 g          477 t
ORIGIN      
        1 gccttccatg atgcgtcagg ctcttgattc acggcaagca gggcctcggg cccccgaccc
       61 tgatacaaga gcacaagcgc cagaaacgct tcggcgctcg cataggacgt gttcagctcc
      121 gacaccttcc ggaatgcgct ctcagcgtcc ttgtagcgcc cttcgatgaa caggtcgagc
      181 cccaagcccc agtatgcgtt ggctgagaga gggtctcgca ccaaacgacg tcgatgagcc
      241 gctatcgagt catcgagccg cccgaacatc caaccgatct ccgccaaatc ctcgtccagc
      301 cgagggttcg gagccagttc gttggctcgg cgcaatgcgg cctcggcgcc tgaccaatcc
      361 cagtcgtggt acttcagcag gtaggctttc gccccatacg cgtccggcga ttcgggatcg
      421 gcgcgaagcg cttgggcgat ggcctcccgc tgcccatcca cattcgacag tccaaaccat
      481 cccagggcgc cgtaagcatc tgcaagtctc gcccaagcgc gcgcatagtt ttggtccagt
      541 gcgatcgcgt ccttgtagag cccgatggcg tgctcggtgt cgggcttggt gttccgcctc
      601 gcaaaaaagt cgccttccag aagcaggtta taggcttgcg tgttcgccgg acgtccttcg
      661 tgcgagtcat tggactccag cgcgacacgt agcgagtccg ccacggttct cgcgatctcg
      721 tcttgaatct tgaaaatatc gctgaagttt ctgtcgtaga tctgcgacca aatatgtgtc
      781 ccgtcagttg ccctgatcaa ctgcacggtg atacgaacat cgtcgcctga ctttcggaca
      841 ctgccttcga gcagattggc gactcccaac ttttgtgcga tccccctcat gtcctcgttc
      901 ttccccttaa actgaaacga cgacgtgcgt gcaatcacct ttagtccggg agcgttggtg
      961 agatggtcga tcaactcctc cgacaagccg tcggagaaat actcttgatc gtggttctcg
     1021 ctcatgtcaa cgaatggcaa tacggcgatc gacctgctcg tcgcgactgg cggctgcaaa
     1081 cgcatccacc cgacgacacc gacgatgagc gcgcacatgg tggccaatag ccatcgtcgg
     1141 ataccgaggg ggacccggcg ccacgcgtgc gccgccccgg caaacattcc ttccgtctct
     1201 ccgatacttg aaatacgtcc gggcctattc ggaccgccgc cagccgtcac tcggaacgcg
     1261 tgcactggct cggccatgtt cttgacggtc ttatctccaa gatctaggaa tgtcgcaggc
     1321 aaccggctcc gtatggaact gcgcactgaa tctgagacga ctactccgcc gggtagagcg
     1381 agttcctgga gacgtgctgc gatgttcacg ccctcgccgt agacacttcc gtcatccttt
     1441 tccagaacgt cgcctagatg cacgccgatg cgaaagcaca agcggcgatc cgatgcttcc
     1501 tctcctgagc tttcctttag cgcttgctgg attcggagcg ccgccctcac ggcacccgcg
     1561 gcggtctcaa agacgacaag gacagagtcg ccggctgtat cgacgattcg accgtggtcg
     1621 gcttcagcgg ctttgcggaa aacgtctcga gctctatcta gcgctagaac agtcgcatgc
     1681 tcgtcgtcga ccatcagtcg cgaataaccc gccgcgtccg ccgcaagaat ggcgaccaag
     1741 tgatgccgga tgccggtgac ttgactcaag cgatgcccat tgtcggaatc tcgccgggtg
     1801 aaggcgggcg aatcccacct cctcggccgc ttgcgggatc ccggcagtcc ggccaatgta
     1861 cctgctcagt ccgtcaggca ggaggcgcca gcgcgcggcc gacctcttga gtcccctggc
     1921 cgtaagctca cgctgtcggc ctgccggcgt agcggctgtt ggagtccaac gaccgtacat
     1981 ggacgccccc ggtttgccaa gctttgagtt cgtgatggtt cggaggtatc gattgcaccc
     2041 gtacatccgg actttcgttg cagcagagct gctgttcctg atgggatttg ctggtcgggt
     2101 cccattcgct ttgtcgcact cgaagtgccg cggacgttct gggctttgcc aaccacggcc
     2161 taacctgtct tgccatcact gcgtgatcac cttagcaatc ggtggttcga tctcctctac
     2221 ttgcctccac g
//
LOCUS       DATMGX010000084         2964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_220845, whole genome shotgun sequence.
ACCESSION   DATMGX010000084 DATMGX010000000
VERSION     DATMGX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220845"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VNR65_01390"
     CDS             <1..499
                     /locus_tag="VNR65_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWJ17357.1"
                     /translation="PETFQYVILYVSLLIAVAAFFGAQRYLDSPLGHMLRAIKSNETR
                     LEYLGTSARRVLLVGYVISAFLGGIGGTLVAIIQQIATPEFGYWTKSGEFVFIAILGG
                     GAHAFGAFAGAAMFEVVRFYAAAQLADAWQLILGVVLIVIILYAPNGLIGLRQRDNRS
                     KEGGH"
     gene            499..1242
                     /locus_tag="VNR65_01395"
     CDS             499..1242
                     /locus_tag="VNR65_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ17358.1"
                     /translation="MANVLLSAKGIKIRFGGVIAADGIDLDVHEGENLAIIGPNGAGK
                     TTFLNISTGYLRPQAGSVSFLGRDITAQLPRTITKLGIARAFQIPQLFLDQTVMENML
                     IAAASLRGHPSGLKALLSLPERPEMERLLDLVGVKEYAERKARELPEGQRKLIDIALA
                     LALKPKLLLMDEPTSGVASSEKFGIMDILITALAKEKVTSVFVEHDMDMVERYASRVA
                     VWSMGKIQKVGTPAEVLADPKVREHVIGI"
     gene            1248..1922
                     /locus_tag="VNR65_01400"
     CDS             1248..1922
                     /locus_tag="VNR65_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ17359.1"
                     /translation="MLSFENINVAIAGAPVLRNVSFSLKAGASAALIGRNGAGKTTTV
                     RTIIGFTEASGIVRLGGQDLGPVPPHRRPSLGIGYAPEDRRLFSAFSVEENILLPGRV
                     AKLSPEETQRRLDRAYSVLPELKELAKRPAGSVSGGQGKMVALGRALMLGTRLVILDE
                     PFQGLAPVLAQRYAEALRRLRSQDKDITLLITESNPKLLDTFADVTLTIERGEIESRQ
                     ATETSD"
     gene            2122..>2964
                     /locus_tag="VNR65_01405"
     CDS             2122..>2964
                     /locus_tag="VNR65_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ17360.1"
                     /translation="MNINVKLPPAKGVFIDNAWRPSHSGRTLAMLAPATGQVIASIAA
                     GDAEDVDLAVKSARRSLEQGAWGRMTATERGRLLSRLGRLIEDHAEELAKLEAADTGK
                     PMKQAKADVTAVARYFEYYGAAADKVHGDTIPFLDGYLVTTLYEPLGVTGHIIPWNYP
                     GQMFGRTVAPALAMGNATVVKPAEEACLVPLRIAELAVEAGFPEGALNVVPGLGEEAG
                     AALAAHPGIDFLSFTGSPEVGTLVQIAAAKNHIGCTLELGGKSPQIVFADADMDAALA
                     SVAAA"
BASE COUNT          572 a          879 c          918 g          595 t
ORIGIN      
        1 accggaaacc ttccaatacg ttattcttta tgtgagcttg ctaatcgcgg tagcggcatt
       61 ctttggcgcg cagcgctacc tcgacagccc tctcggacac atgctgcggg caatcaagtc
      121 gaacgagacg cgtctcgaat atctcggaac gtcggcccgc cgcgtgcttc tcgtcggcta
      181 tgtgatctcc gcgttcctcg gtggtattgg cggcacgctg gtggcgatca tccagcagat
      241 cgccacgccg gagttcggct actggacaaa gtcgggcgag ttcgtgttca tcgcgatcct
      301 cggcggcggc gcgcatgctt tcggtgcgtt tgcaggagct gccatgttcg aggtcgtccg
      361 cttctatgct gccgctcagc tcgcagacgc gtggcagctc attctcggcg tggtgctgat
      421 cgtcatcatt ctctacgcgc cgaacggcct gataggcctc aggcagcgcg acaatcgttc
      481 gaaggaagga ggtcactgat ggccaacgtc cttctctcgg ccaagggcat caagatccgc
      541 ttcggcgggg tcatcgcagc cgacggaatc gacctcgatg tccatgaggg cgagaatctc
      601 gccatcatcg ggcccaacgg cgccggcaag acgacgttcc tcaatatctc gaccggttac
      661 ctcaggccgc aggcaggcag tgtgagcttt cttggccgcg atattaccgc gcagctgccg
      721 cgtacgatca ccaagctcgg catcgcccgc gcttttcaga ttcctcaact gttcctcgat
      781 cagacggtga tggagaacat gctgatcgcg gccgcctcgc tcagggggca tccatcgggg
      841 ctcaaggctc tcttgagcct gccggagagg ccggagatgg agcggctgct cgatctcgtg
      901 ggcgtcaagg aatacgcgga gcgcaaggcg agagaattac ccgaagggca gcgcaagctc
      961 atcgatattg ctcttgcgct ggccttgaag cccaagctcc tgctcatgga cgagccgacc
     1021 tcaggcgtcg cgtcgtcgga gaagttcggc attatggaca tcctcatcac cgcgctcgcg
     1081 aaggaaaagg tgacctcggt ttttgtcgag cacgatatgg acatggtgga gcgctatgcc
     1141 agccgcgttg cagtctggag catgggaaag attcaaaaag ttgggacgcc tgcggaggtc
     1201 ctggccgatc cgaaagtccg cgagcacgtg atcggaatat gatgccgatg ctgagctttg
     1261 agaacatcaa cgtcgcgata gccggtgcgc ccgtgctgcg caatgtcagc ttctccttga
     1321 aggccggcgc gagcgctgca ctcatcgggc gcaatggcgc tggcaagacc acgacagtac
     1381 gcaccatcat tggcttcacg gaagcaagtg gcatcgtgcg tttgggcggc caggatcttg
     1441 ggcccgttcc gccacatcgt cggccatccc tgggtattgg ctatgcgccc gaggatcgtc
     1501 gcctgttttc agcattctct gtggaggaga acatccttct cccgggccgc gttgcgaagc
     1561 tgagtcctga ggaaacccag cgtagactgg atcgcgccta ttccgtgctg ccggagctga
     1621 aagagcttgc caagcggccg gctggatcgg tttccggggg ccagggcaag atggtggcgc
     1681 taggccgcgc tctgatgctg ggaactcggc tcgtcattct cgacgagccg ttccagggtc
     1741 tcgctcctgt gctggcgcag cggtacgcag aagcgctccg gcggcttcgt tcgcaggaca
     1801 aggacatcac gctgctcata acggagtcga acccgaagct tctcgacacc ttcgccgacg
     1861 tgacgctaac catagagaga ggcgagatcg aaagccggca agcaactgag acaagcgact
     1921 aggatgccct tctgagcggc gcgacgtcta cgtgctgtcg cgcggccgtg tcgtgatgca
     1981 cgctgttccc ggtgacatta ccgaggagcg gctgcaccaa gtgcatttga attgagttgg
     2041 accgttcatc cgcaccgttc cgaagacgag gcattcgctt tttaggacgc gcaaccagaa
     2101 caataagcaa ggagagttcg tatgaatatc aacgtcaaac ttccgcccgc gaaaggcgtt
     2161 ttcatcgaca acgcatggcg gccttcgcat tcgggtagga ctcttgcgat gctggccccc
     2221 gccacaggcc aggtcatcgc ctcgattgcg gcgggcgacg ccgaagatgt agacctcgct
     2281 gttaagtcgg cgcgtcgctc tcttgagcag ggtgcgtggg gccgcatgac cgcaacggag
     2341 cgcggccggt tgctctcgcg gctcggcagg ctgatcgaag atcatgccga ggaacttgcc
     2401 aagttagaag cggcagacac cggcaagccg atgaaacagg ccaaagccga cgtcaccgcg
     2461 gtcgctcgct attttgaata ttacggcgca gccgcggaca aggtgcacgg cgacactatt
     2521 ccatttctcg acggctacct cgtcaccacg ttatacgaac cgctcggtgt gaccggacat
     2581 atcattcctt ggaactatcc gggtcaaatg ttcggccgaa ctgtggcgcc ggctcttgcg
     2641 atgggcaatg cgacagtggt caagccggcc gaggaggcct gcctggtgcc gctgcggatc
     2701 gccgagcttg cagtcgaagc aggatttccg gaaggtgcgc tcaacgtcgt tcccggactt
     2761 ggggaggaag cgggcgccgc gctcgccgcc caccccggaa ttgacttttt gtcttttacc
     2821 ggtagtccgg aagtcggcac cctggtccag atcgccgcag ctaaaaacca tatcggttgc
     2881 acgctcgaat tgggcggcaa atctccccag atcgtgtttg cagacgctga catggacgca
     2941 gcgcttgctt cagtggcagc cgcg
//
LOCUS       DATMGX010000085         4196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_277936, whole genome shotgun sequence.
ACCESSION   DATMGX010000085 DATMGX010000000
VERSION     DATMGX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277936"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..662)
                     /locus_tag="VNR65_01410"
     CDS             complement(357..662)
                     /locus_tag="VNR65_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17361.1"
                     /translation="MLKHSFAILAISTIPAMAADNIVSSHDVQFDQVSFTCGEMNQGG
                     KVVRFIHSDPGAKYIQEVQPAADDPLAKSWDITYRIICEGGYQHPISAHAGSRGSLR"
     gene            1081..2565
                     /locus_tag="VNR65_01415"
     CDS             1081..2565
                     /locus_tag="VNR65_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWJ17362.1"
                     /translation="MRRRALGAFLVIVAGLIALPSPQQGKAEAAGTDTSKHFELFSDV
                     LAKVRANYIVKPDDSKLLEDAIKGVLTELDQTRGGPMALPLPRQGKTVAFGDVLAQIR
                     ANYPVRTDNSKLVEYAIKGAVSGLDPHSTYLSPKEVEEEQDEARGGFGGLGVNVRMES
                     GGAKVIEPLDTMPAERAGVLAGDTITAIDGRSLKGLSMSAAGEKMRGPVGSAVTLTLA
                     RNGVATPITVKVVRDIIRVNPVQYSVEGDVGWIKVKSFEHRYTADRVRWAVEDIKKVL
                     GSKVSGYVLDLRNNPGGLLDQAIAVADAFLDGGSIVMTKGRNASDSQRADAKAGDITD
                     GKLLVVLINGGSASASEVVAGALQDNNRATLLGTRSYGKGSVQTLIPLVNRGAIRLTT
                     ATYYTPSGRSIQATGIEPDYDLKPEIPQSLKLELASLPFESEAQLRGHLKNHGAQERT
                     GSGTYVPKEKAMDDQLNAAIALIHALLPDADQNADGSGSGSSRS"
     gene            complement(3248..3826)
                     /locus_tag="VNR65_01420"
     CDS             complement(3248..3826)
                     /locus_tag="VNR65_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17363.1"
                     /translation="MTRVEILALIETLNAELLSHSSATLTLERWCSGHGLSTEAKLVA
                     RLERGTQKHLEERDRERLAVQTDDQLRYRHVRLFCGDKLLSEADNWYVPSRLTAEMNR
                     LLNETDMPFGRVVHDLDFRRETLSAKLLWSPLPERWDVSPEPLLPPPGSRLEAPDHIL
                     EHRAILRANGGTPFSFVVETYTRDVLAFPLPH"
BASE COUNT          942 a         1159 c         1233 g          862 t
ORIGIN      
        1 cgaactatct cggctggcgc cgcatgttcg agcgcgaagg cgataccatc accgcacagc
       61 gttgcctctt ggcggctttg gcataaaccg caacacttaa catcaacagc gcctcggatt
      121 aacacatcgg cgctgcgacg aacgctttac cataaggcgt gcggtgagcg ccgtccaaga
      181 tggcatggcg agattggtga agcatgccta tcgttcaatc ggacacggga gtcatcttag
      241 gagtatcggg ggagtgctca ggaataaggt tggccggatt tcacccagtc agctccagct
      301 taaggacaaa cggaatgaga aaagctgaaa ccgactggag cgctcagcgt atgcgcctag
      361 cggagagagc ctctgctgcc agcatgggcg ctaatcgggt gctgatagcc gccttcgcag
      421 ataatccggt aggtgatgtc ccaactcttg gccagcgggt catccgctgc aggctggacc
      481 tcttggatgt attttgcacc ggggtcagaa tgaatgaagc gcacaacctt gccgccctga
      541 ttcatctctc cacaggtaaa ggagacctgg tcgaactgca cgtcatggct ggagacgata
      601 ttgtcggctg ccatcgctgg aatggtggat atggctaaaa tcgcgaatga atgtttcaac
      661 atgggagcct ctctccgtct cgcgttcctt agccggggtt gcggctctgg agccggaatt
      721 cagggctcgg gggccgaaac tctagccaga tcgagacctg accgtatttc aatgccatca
      781 cggttctgag tttgacttaa ggcaaactga acgttgactt aaatcaatat taatgcggag
      841 tgcgctccgg caaaatcacg ttctcgtgat gagggagtca ggtcagatcg aaactgactg
      901 tgctttcccc tgtcgcgcgc cttcggcgtc tggcttaaat caaactgaac gcttcctttc
      961 gtcgaactta atagacaggg cgcgttcgaa cccggacact gcaaagagat ggcgaagtga
     1021 tcatttcttt cgcgcgaagc gcaattgagc acttggtaat taccaaaagg tgagcaaata
     1081 atgcgtagac gcgcgctcgg tgcgttcctt gtgatcgtag ccggtctcat cgctctgccc
     1141 tcgccgcagc aggggaaggc cgaagcggcg gggaccgata cctccaagca tttcgaactt
     1201 ttcagtgatg tgctcgcgaa ggtgcgcgct aactatatcg tgaagcctga cgattcgaaa
     1261 ctcctcgaag acgcaattaa gggcgtcctg acggagctgg accagacgcg cggcggtcct
     1321 atggcgctgc ccttgccgag gcaggggaag actgtagcgt tcggagatgt gctcgcgcag
     1381 atacgcgcca attatcccgt gaggaccgat aattcgaagc tggtcgaata cgcaatcaag
     1441 ggcgcagtgt cggggctgga cccgcattcg acctacctct cgccgaaaga agtcgaagaa
     1501 gagcaagacg aggcgcgcgg cggttttggc ggtcttggcg tcaacgtgag aatggagagc
     1561 ggcggtgcga aggtcatcga gcccctcgac acgatgcccg cggaacgggc aggcgtgctt
     1621 gcgggcgaca ccataaccgc tattgacggg agatctctca aaggcctcag catgagcgcc
     1681 gccggggaga aaatgcgcgg gccggtgggg tctgccgtaa cgctgacgct cgccagaaac
     1741 ggcgtcgcca cgccgatcac cgtcaaggtc gtccgcgaca tcatccgggt taatcccgtg
     1801 cagtatagtg tcgaaggaga cgtgggctgg atcaaggtca agtcgtttga acatcgatac
     1861 accgccgatc gtgtccggtg ggcggtagag gacataaaaa aggttcttgg ttccaaggtt
     1921 tccggctatg tccttgatct caggaacaat ccaggtggcc ttctcgatca ggctatcgcc
     1981 gtcgcggatg catttcttga cggcggttct atcgtcatga cgaaaggccg taacgcctcc
     2041 gatagtcaac gcgccgacgc gaaggccgga gacatcacgg atggaaagct gcttgttgtg
     2101 ctgatcaacg gcggatcggc ttccgcttcg gaagttgtcg caggcgcgct gcaggacaac
     2161 aacagggcta cgctcctcgg caccaggtcc tatggcaagg gctcggttca gacgctgatc
     2221 cctctcgtga acaggggggc tatacgtctg acaaccgcga cgtattacac cccctcgggc
     2281 cgttccatcc aggcaacagg catcgagccg gattatgatc tcaagcccga aattccgcag
     2341 agcttgaagt tagagcttgc ctcgctgccc ttcgaatccg aggcgcaact tcgcggccat
     2401 ctgaagaatc acggcgccca ggaaagaacc ggcagcggta cgtatgtacc gaaagaaaaa
     2461 gcgatggacg atcaactcaa tgcggccatc gccctaattc atgccctgct cccggatgcg
     2521 gaccagaatg cagatggatc aggttcgggc tcctctcgca gttaacccgg taatttccgt
     2581 cgcggcgttt cgggcagatt aggaactccg tagtccattc gggtcttgtg taacgagcat
     2641 tgccagtcaa aggcatagcg gtcttgcttc tgctttatgc atgcgttgtg caaactatat
     2701 gcatgcacaa gcccttatcg ccgtcgcaca aggttgacag cgttttgaca gcagacttcc
     2761 gctaactttg ttgtctcctt aggagacacg ctgctgtcag cattccataa acggccacga
     2821 tggtcgtgct gtcggcatcg caaacctaga cacaaacttc cggggcgacg ccgtgaaagc
     2881 ggctcgcacc ggcttttatg acgtttgcat caacgcggtc tccaggctgt acgcgctcca
     2941 gacgaacaat gagacgtcgc atttagtgcc cgacaacttg agacgccgta atacctgcca
     3001 ctagactgga aatggtgtgg ttatgcccct tggcggggcg ggttgcaaac tcagtcgggg
     3061 tgagccagtg gaggcttgtg tgcgggctct tatgttgtag tcgagacgcc agctttcgat
     3121 gatcaggcgc gcctgcttca gactggcgaa ggatctacaa tcggccttaa gcgtcaaaat
     3181 tcaccgaagg ccattgctgt cctgtaagcg gatgcaaatg ccggaaccgg ctactggaac
     3241 gcggttttta gtgcgggaga ggaaaagcca acacgtcccg cgtgtaggtc tccacgacaa
     3301 aagagaaagg tgtgccgcca ttcgcgcgca ggatcgcccg gtgctcgagg atgtgatcgg
     3361 gcgcttccag gcggcttcca ggaggcggga gcaagggctc cggcgaaaca tcccagcgct
     3421 cggggagcgg cgaccacaag agcttcgccg atagcgtttc gcggcgaaaa tcgagatcgt
     3481 gaaccacccg gccaaaaggc atgtcggttt cattgaggag gcggttcatt tcggcggtca
     3541 gccggctggg cacataccaa ttgtcggctt ccgacagaag cttgtccccg cagaaaagcc
     3601 gaacatgacg gtaacgaagc tgatcgtcgg tctgtaccgc cagccgctca cgatcccttt
     3661 cctcaagatg cttctgcgtg ccgcgctcaa ggcgggcaac cagcttcgcc tctgtggaaa
     3721 ggccgtgtcc gctgcaccag cgttcaagcg tcagggtagc gcttgaatgg ctcaagagct
     3781 cggcattgag tgtttcgatc agagccagga tctccacgcg cgtcaaaaag ctatcctgcc
     3841 agctcaacgg aacaagcccc aaaagccaag gacagacgga gacaataaag aaccatcgcc
     3901 agagcaccat cagttgcctt ggttgagtga cttttcatca ccggtactgg agcagcccgc
     3961 ttccatttgg acgcggataa gctgctctag cgcgttataa tccatcactt ttccgccttt
     4021 aatcgattga aggcgggttg atccagggct tagcggcagc gattcaactg ccctttggct
     4081 aagtacacct caggcaaggc gaggcggaag cgccatctcg ccgcagaccc gcaacgtttg
     4141 cgtgaacgct gaggcagccc cgaacctctc tcaattcggt gccggaagga tgtccc
//
LOCUS       DATMGX010000086         3759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_410420, whole genome shotgun sequence.
ACCESSION   DATMGX010000086 DATMGX010000000
VERSION     DATMGX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410420"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VNR65_01425"
     CDS             complement(<1..601)
                     /locus_tag="VNR65_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HWJ17364.1"
                     /translation="MNEKKNRTQVKARLPKGLRDVEAQEIRAQQQMLRKISDVYERYG
                     FEPLDTPAFEYTDALGKFLPDTDRPNEGVFSFQDEDEQWVSLRYDLTAPLARYVSQNF
                     DALPKPFRRYQSGYVYRNEKPGPGRFRQFMQFDADTVGTANPAADAEMCMLAADTLEA
                     LGIPRGGYGIKVSSRTLLDALLDSAGITDARQRLVVLRAV"
     gene            complement(772..951)
                     /locus_tag="VNR65_01430"
     CDS             complement(772..951)
                     /locus_tag="VNR65_01430"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HWJ17365.1"
                     /translation="MSLFAHIEELTEKHQSLQRQIEEEMNHPLVDTLKLTELKRKKLR
                     LKEKIEKLKAERKVA"
     gene            1241..2221
                     /locus_tag="VNR65_01435"
     CDS             1241..2221
                     /locus_tag="VNR65_01435"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWJ17366.1"
                     /translation="MTIEKRPVRADDDGLRLDRWLKKYFPSLPHTLVHKLVRTGQVRV
                     DGGRVKAEARLCAGQEIRIPTHFAAPQAEPVPSLRPPPGLSKADRDFIERMIVFENDD
                     IVILNKPFGIAVQGGTKTVRHIDGLLAGMADRFGGERPRLVHRLDRDTSGILVVAKDR
                     QTAANLGRLFQTRSVQKIYWAVTQGIPKPPQGKVEAALVKAAGPDGDRVRKAKPGEQE
                     LAQHATTYYSVIDRSGKMAWLSLKPVTGRQHQLRAHMDVLGTPILGDQKYGSVADLPM
                     DGIEQKLHLHARRLTLKDAKLGEIDVTAPLPPHMAKTFELLGFDAQQYGE"
     gene            2342..3043
                     /locus_tag="VNR65_01440"
     CDS             2342..3043
                     /locus_tag="VNR65_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWJ17367.1"
                     /translation="MTSTPILIVFDCDGTLADSQHIIAEAMRFAFRSAGLAAPERSSI
                     LRIVGLSMGEAVAALAPDQGEARRAEIVRAFRERSTALRQTATMREPMFPGADSLLRS
                     LVEREDVILGLATGKSRRGVARIIEQHGLDGIFATIQTADDAPSKPHPAMLLQAMDET
                     GTSPATAVMIGDTSYDILMAASANVASIGVTWGYHTKAELRQAGAKTIVHSFAELERA
                     LDGRAVFARNYEAVA"
     gene            3131..3205
                     /locus_tag="VNR65_01445"
     tRNA            3131..3205
                     /locus_tag="VNR65_01445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3163..3165,aa:Val,seq:cac)
     gene            complement(3272..>3759)
                     /locus_tag="VNR65_01450"
     CDS             complement(3272..>3759)
                     /locus_tag="VNR65_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWJ17368.1"
                     /translation="GQIGYNFQTGAFVFGVETDFQGAGITNSVTGTTAKGNDFTSKES
                     VDWFGTTRGRLGFAVGNALLYGTGGFAYGNVNQRATYNGDAFASNTTQTGYAAGGGIE
                     YKFNPAWSLKAEYQFIDLGSQKLTDTLGYTTNPLDTNFQTARVGLNYRFGGGGGYEPL
                     K"
BASE COUNT          744 a         1078 c         1143 g          794 t
ORIGIN      
        1 ccacagcccg gagcacaacg agacgctgcc gggcgtctgt aatgcctgcg ctgtccagga
       61 gagcgtcgag caaggtccgg cttgatacct tgatgccata accaccgcgg ggtatgccaa
      121 gcgcctccag cgtatcggcc gcgagcatgc acatttccgc atcggcggcc gggttcgccg
      181 tacccaccgt atcagcgtcg aattgcatga actggcggaa gcggcctggc ccgggctttt
      241 cgttgcgata gacgtagccg gattggtagc gccggaaagg cttcggcagc gcatcgaagt
      301 tctgcgacac gtagcgggcc agcggcgcgg tcaagtcgta gcgcagcgaa acccactgct
      361 cgtcctcgtc ctggaacgaa aacacacctt cgttcggccg gtccgtgtcg ggcaggaact
      421 tgccgagcgc gtccgtatat tcgaaagctg gcgtatcgag cggttcgaaa ccgtaccgct
      481 cgtacacgtc gctgattttg cgcagcattt gctgctgggc acgaatttct tgcgcttcaa
      541 cgtcgcgcag gcctttaggg aggcgcgcct tgacctgggt gcggtttttc ttctcgttca
      601 tgcgggaaga aatgcagagg tgcgcccctt aacgcaaccg atttgttgaa taaatccgga
      661 tttgttaaag aaacccggag actggctcag cggctgcaag gcgcaacaac gttcttacca
      721 ggttcgcccg gcggagccgc tgtcaggttt gttcggaagg ggaagggtgg cttaagccac
      781 cttgcgctcg gctttgagct tttcaatctt ctctttgaga cgaagttttt tgcgcttgag
      841 ttcggtgagt ttcagcgtat cgacgagcgg atggttcatc tcttcttcga tttggcgttg
      901 taggctttgg tgcttctccg tcagttcctc aatatgcgca aaaaggctca tttcaacggc
      961 ctcctacttc tgtttagcac tacatggcac ctctatatta tggcaaaacg atgacaaaaa
     1021 taagccaaaa atagggcgag gttgcgggaa tcgcctaaag caactattcg aattttctaa
     1081 attcgctcgg cctatcaatg cggtggtcgt ccctatacaa cttatggcta agccctccct
     1141 tacaaagcgg cttgaccggc gcgcatttag acgaggatgc aagcgctttt tcgagagtgt
     1201 tcctctttac tttgggcctg cgctggcgaa aggtgcggcc atgaccattg aaaagcgccc
     1261 tgtccgggcg gacgacgacg gcctccgtct cgaccgctgg cttaagaaat attttccgtc
     1321 gctgccccat acgctggtcc acaagctggt gcgaaccggt caggttcgcg tcgacggcgg
     1381 tcgtgtgaag gctgaggccc ggctttgtgc cggacaggag atccgtatcc cgacccactt
     1441 cgccgcgccc caggcggagc ctgtgccgtc cctgaggcct ccgccgggcc tatcgaaggc
     1501 cgaccgcgat ttcatcgagc gcatgatcgt cttcgagaac gatgacatcg tgatcctgaa
     1561 caagcctttc ggcatcgcgg tgcagggggg caccaagacg gtgcgtcaca tcgatggctt
     1621 gcttgccggg atggcggacc gcttcggcgg agagcgcccc cgtctcgtgc accgcctcga
     1681 ccgcgacacc agcggcatcc tggtggtcgc caaggatcgc cagaccgcgg ccaacctcgg
     1741 taggctcttt cagacccgct cggtccagaa gatatactgg gccgtgaccc agggcatccc
     1801 gaagccgccg caaggcaagg tcgaagccgc gctcgtgaag gccgccggcc ccgatggcga
     1861 ccgcgtgcgc aaagcgaagc caggcgagca agagctggct cagcacgcca ccacctatta
     1921 ttcggtgatc gaccggtccg gcaaaatggc gtggctgtcg ctgaagccgg tgacagggcg
     1981 gcagcatcag ctgcgcgctc acatggacgt cttggggacg ccgatcctcg gcgaccaaaa
     2041 atatggaagt gtagccgatt tgccaatgga cggcatcgag cagaagctcc atcttcatgc
     2101 aaggcgtctc accttgaaag acgccaaact gggagaaatc gacgttacag cgccgttgcc
     2161 gcctcacatg gcgaagacgt tcgaactgtt gggctttgat gcgcagcagt acggcgagta
     2221 acttctcatc cactatagcg tgaagtcagt cgaaattgtc tcgatatcgt ttatgtgggg
     2281 tagagcgagt gcatggatga gatgtctgag cgctaaacgg aacctgctta ttgggaaaag
     2341 aatgacctcg acaccaattc ttatcgtttt cgattgcgac ggtacacttg ccgacagcca
     2401 gcatattatt gcggaagcga tgcggtttgc tttcagaagc gccggtcttg ccgcgccgga
     2461 gcgcagcagt attttgcgta tcgtaggcct ctcgatgggc gaggcggttg ctgccctggc
     2521 cccggatcaa ggcgaggcga gaagggcaga gatcgtgcgc gccttccggg agcgaagcac
     2581 ggctttgcgc cagaccgcga ccatgcgtga gccgatgttc ccgggtgcgg attcgctttt
     2641 gcgctccctt gtcgagcgtg aggatgtcat cctcggccta gccaccggaa aatcgagacg
     2701 cggtgttgcg cgcatcatcg agcaacacgg cttggacggc attttcgcca cgatccaaac
     2761 cgccgacgac gcgccatcga agccacatcc ggcgatgctc ctccaggcga tggatgagac
     2821 gggaacttcg cctgcaacgg ccgttatgat cggagatacc tcttacgaca ttctcatggc
     2881 tgcatccgcg aatgtcgcca gcatcggggt tacctggggc tatcacacca aggccgaact
     2941 gcggcaggct ggggcgaaga caatcgtgca cagcttcgcc gaattggagc gcgctctcga
     3001 tggccgtgcc gtattcgctc ggaactatga ggctgtggcc tgaccttgtt tatctcctgg
     3061 gaaggctgcc ggtgacctga gccgattgtg ttgacagtcc ggcggccaac cccttagata
     3121 gcgcaactct ggacacgtag ctcagcggta gagcattcgc ttcacacgcg aagggtcaga
     3181 ggttcgatcc ctctcgtgtc caccatccaa ccactcatga cgaggaaacg gcgcccggag
     3241 gcgccgccct gcagagcgtt gtgggagcag tctacttcag aggctcatat ccgccgccac
     3301 cgccgaagcg atagttcaga ccgacgcggg cggtttggaa gtttgtgtcg agcggattgg
     3361 tggtgtatcc gagggtgtcg gtgagcttct ggctgccgag atcaataaat tgatactcgg
     3421 ccttgagaga ccaggcagga ttgaatttgt attcgatgcc gccgccggcc gcgtaacccg
     3481 tctgcgtggt attgcttgcg aaggcatcgc cgttgtaagt ggcgcgctga ttgacgttgc
     3541 cgtaagcgaa gccgccggtg ccgtagagga gtgcgttgcc gacggcaaag ccgagccgcc
     3601 cgcgagttgt gccgaaccag tccacgctct ccttgctggt gaagtcattg cctttggccg
     3661 tcgtaccggt cacgctattg gtgatcccgg cgccttggaa gtcggtttca acgccaaaga
     3721 cgaaggcgcc ggtttggaag ttatagccga tttggccgc
//
LOCUS       DATMGX010000087         4690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_17294, whole genome shotgun sequence.
ACCESSION   DATMGX010000087 DATMGX010000000
VERSION     DATMGX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17294"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VNR65_01455"
     CDS             <1..198
                     /locus_tag="VNR65_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17369.1"
                     /translation="LDASAAPRQALPIALLQAPDHNMAAASAMIQEPGTVWRKMIEAK
                     QVQAPDKAPQRNCGGDGIAPL"
     gene            complement(193..2952)
                     /locus_tag="VNR65_01460"
     CDS             complement(193..2952)
                     /locus_tag="VNR65_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HWJ17370.1"
                     /translation="MSTISATKPASTSAIKADPSSDVYRSESHPLDPLFSPRSVAVIG
                     ASERAGSVGRSVLWNLLSSPFGGTVFPVNAKRTNVLGIKAYPSVKDLPDKVDLVVITT
                     PADTVPDLIAESVELGIPTAIVISAGFKEHGEHGRELERQISEIIRGKMRLIGPNCLG
                     VMNPVRGFNATFAHTVARPGNVAFISQSGALCTAVLDWSLRENVGFSAFVSVGSMLDV
                     NWGDLVDYFGNDGRTHSIVIYMESIGDARSFLSAAREVSLTKPVIVIKAGRTAAAAKA
                     AASHTGSLTGSDEVFDAAFRRSGVLRVNSIADIFFMSDVLAKQPRPRGKRLCILTNAG
                     GPGVLATDALVAGGGELAELSPATIKAFDEILPAEWSHNNPVDILGDAEPERYAKSLE
                     IAAKDPNIDGMLVVLTPQDMTNPTQIAEKLKPYAKGFGKPVLASWMGGAEVAAGEQIL
                     NQAGIPTFQFPDSAVRAFNYMWRYSYNLKGLYETPALPQHADAAVQRGIAEHIIQQAR
                     QSGRTILTEYESKQLLKAYDIPTVDTRIAVTEQEALQAAEEIGYPIVLKLYSLTITHK
                     TDVGGVQLNLHDAGAVKTAFQVIKQSVTEKVGAEQFQGVTVQPMVKLDGYELIVGSSL
                     DAQFGPVILFGTGGQLVEVFKDRALALPPLNSTLALRMMEQTNILKALKGVRGRKPVD
                     LAALEDLLVRFSQLVVEQPWIEEIDINPLLASSERIVALDARVVVHGLDMTEDQLPTT
                     AIRPYPIHLVRPWQTKDGKDVVIRPIRPEDEPMMIEFHKKLSERSVYLRYFQPLKLTQ
                     RTAHERLTRTCFIDYNREMALVAERKNEAGEPEILAVGRLSKLHGRDEAELAAVATDA
                     AQHKGLGTELYRRLIEFARDEKLKLVVSNMLPENREMRAVCVKHGFKMFSDMEDNMIR
                     AELEL"
     gene            3282..4334
                     /gene="purM"
                     /locus_tag="VNR65_01465"
     CDS             3282..4334
                     /gene="purM"
                     /locus_tag="VNR65_01465"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWJ17371.1"
                     /translation="MAQQTTETRIITYADAGVDIERANRTKQRIKYLAHKTFNRGVLS
                     EIGGFGGLFAIDKKKYLDPVLVSSVDGVGTKLKIAFEMNLHHTVGADLVNHCVNDIAV
                     QGAAPMFFMDYFATGKLQPEIAEKVVAGIADACKQNGCALIGGETAEMPGFYGNGEYD
                     LAGFIVGVANRDKLITGKAVQAGDVLIGLSSTGLHTNGYSLARKLFFSVAHYTPDSYV
                     NEIKGKVGTELMKTHKSYWPIMRRMIEADAVSAMAHITGGGITENLPRALPKGVGAIV
                     EINSWPVPPIFEHLQKIGNVPQDEMLRTFNMGIGMILVVPANKFKKVQTILERCGEKG
                     HTIGRIVKGEKRVTYQ"
BASE COUNT          973 a         1413 c         1373 g          931 t
ORIGIN      
        1 ttggacgcgt ctgccgcacc gcggcaagcg ctgccgattg cacttctgca agctcccgat
       61 cataacatgg cagccgcgag cgccatgata caggagccgg ggacggtgtg gcgaaaaatg
      121 atagaggcaa agcaggtgca agccccggac aaagcgccgc agcggaactg cggcggcgat
      181 ggcatcgcgc cgttatagtt ccagctcggc ccgaatcatg ttgtcttcca tgtccgagaa
      241 catcttgaac ccgtgcttga cgcacacggc cctcatttcg cggttttcgg gaagcatgtt
      301 ggagaccacc agtttgagct tctcgtcgcg ggcaaattcg atcaggcgac gataaagttc
      361 cgttcccaga cccttatgct gagcggcatc cgtggccact gcggcgagtt cggcttcatc
      421 gcggccgtgc aacttgctaa gacggccgac cgccagaatc tcgggttcgc cggcctcgtt
      481 cttgcgctcc gcaaccagcg ccatctcgcg gttgtagtca ataaaacaag tgcgggtcaa
      541 gcgctcgtgg gcggtgcgct gggtcagctt caacggctgg aagtaccgca gatagacgct
      601 tctttccgag agctttttgt ggaactcaat catcattggc tcgtcttcgg gccggatagg
      661 cctgatcacc acgtccttcc cgtccttggt ctgccatggc ctgaccaggt gaatcggata
      721 gggacgaatg gctgtggtgg gcagctggtc ttccgtcata tccagaccat ggaccaccac
      781 ccgcgcgtcc aacgcgacga tacgttccga tgaagcgagc agcgggttga tgtcaatttc
      841 ctcgatccac ggctgctcaa cgaccagttg gctgaagcgc accaggagat cttccagcgc
      901 cgccaaatcg accggcttgc gtccgcgaac tcctttcagc gctttgagga tgttggtctg
      961 ctccatcatt cgcagggcga gcgtggaatt caaaggcggc agggcaaggg cgcggtcctt
     1021 gaagacctcg accaattgcc cgccggtccc gaacagtatg accgggccga actgcgcatc
     1081 gagactgctg ccgacgatca actcgtagcc atcgagcttc accatgggct ggaccgtaac
     1141 gccctggaat tgctcggcgc caaccttttc ggtgaccgac tgcttgatga cttggaacgc
     1201 ggtcttcacc gcacccgcat cgtgcaggtt caactgcacc ccgcccacat ccgtcttgtg
     1261 cgtgatggtg agcgagtaca gcttcagcac gatgggatag ccaatctcct ccgcagcttg
     1321 cagcgcttcc tgttcggtga ccgctatccg ggtatcgacc gtgggaatgt cgtaggcctt
     1381 gagcaactgc ttcgattcat actccgtcag gatggtgcgg cccgactgac gggcctgctg
     1441 gatgatgtgt tcggcaattc cgcgttgcac ggcagcgtca gcatgttgcg gcaacgccgg
     1501 agtttcatac agccccttca ggttgtagga gtaccgccac atatagttga aggcgcgcac
     1561 cgcgctatcg ggaaactgga aggtcggaat gcccgcctgg ttcaggatct gctcgcccgc
     1621 cgcgacttcc gcgccaccca tccagcttgc cagcaccggc ttgccaaatc ctttggcata
     1681 cggcttcagc ttctcggcaa tctgggtcgg gttggtcatg tcctgcgggg tcagcaccac
     1741 cagcatgccg tcgatattgg gatctttggc ggcaatctcc agcgacttcg cataacgctc
     1801 aggttcggcg tcgccgagga tgtccaccgg gttgttgtga ctccactcag cgggaagaat
     1861 ttcgtcgaat gcctttatcg ttgcgggcga caattcagcc aactcgcctc caccggcaac
     1921 cagcgcgtcg gtggccagca caccgggacc gcccgcattc gtcaggatgc acagccgctt
     1981 gccccggggc cgtggctgct tggccaacac gtcggacata aagaaaatgt cggcgatgct
     2041 gtttacccgc agtacgccgc tgcgccggaa tgcggcatcg aacacttcat cgcttccggt
     2101 aagcgatccc gtgtgtgaag ccgccgcttt ggctgcggca gccgtgcgtc cagccttgat
     2161 cacaatgacc ggcttggtca gcgaaacctc gcgcgcggcg gagaggaacg agcgcgcgtc
     2221 accaatggac tccatgtaga tcacgatgct atgggtgcgc ccatcgttgc cgaagtagtc
     2281 caccaagtcg ccccagttca cgtcgagcat ggagccgacg gacacgaacg cgctgaagcc
     2341 aacattctcg cgcaggctcc agtccagcac ggccgtgcat agggcaccgc tctggctgat
     2401 gaaagcgacg tttcccggcc gtgcaaccgt gtgggcgaag gtcgcattga acccgcggac
     2461 ggggttcatt acccccaggc agttggggcc aataaggcgc atcttgccgc gaataatctc
     2521 cgagatctgc cgctccagct ctcttccgtg ctcaccgtgc tccttgaacc cggcggagat
     2581 cacgatggca gtggggatgc ctagctccac cgattcggca atgaggtcgg ggacggtatc
     2641 ggccggggta gtgatcacga ccaggtccac cttgtcggga aggtctttga cgctgggata
     2701 ggccttgata ccgaggacat tggttcgctt ggcattgacc gggaacaccg tcccgccaaa
     2761 ggggctgctc agcaggttcc acagcacgga acggcccaca ctgccggccc gctccgacgc
     2821 gccaattacc gcaacgctgc ggggtgaaaa caagggatcc agaggatggc tctcggaccg
     2881 gtaaacgtcg gatgaaggat cggctttgat ggcgcttgta cttgcgggtt tagtggcaga
     2941 aatcgtgctc atgcttcctc acaggatcaa attccagttt aagccctggc ggattgcgcc
     3001 gggagcaact cgtcacagaa agtcgtgatt cggatcacaa aatttccggc tccggcacct
     3061 cgcaaaaaat ggatcgggaa gaccgtaatc ggccgggtcg tgaaaatccg cggttgcggt
     3121 gtgccctctc tttccgaggc cgtgacttag ttaactcctt tccctccttt gtcctccgtg
     3181 ccggaaacag ccatcgcagc ccacccgaaa cgcggaatga tacaatgcct cttcccgcgc
     3241 aaaaatctgc ggcgatgcgc cctctgactg gaccccgact cttggcacaa cagacgacag
     3301 aaacccgaat catcacctac gccgatgctg gcgtggatat tgagcgcgcc aatcgcacca
     3361 agcagcgcat caagtatctt gcccataaga cattcaaccg cggcgtgttg agcgagatcg
     3421 gcggcttcgg cgggctcttc gccatcgaca agaaaaagta cctggatccc gttctggtct
     3481 ccagcgtgga cggagtcggc accaagctga aaatcgcctt cgaaatgaac ctgcaccaca
     3541 ccgtcggggc cgacctggtg aaccactgcg tgaacgacat cgccgtgcag ggcgccgcgc
     3601 ccatgttctt catggactac ttcgcgaccg gcaagctgca gccggaaatc gccgaaaagg
     3661 tcgtggcggg catcgccgat gcctgcaagc agaatgggtg tgcgctcatt ggcggcgaga
     3721 ccgccgaaat gcccggcttc tacggcaacg gcgagtacga tttggcggga tttatcgtcg
     3781 gcgttgcgaa ccgcgataag ctcatcaccg gcaaagcggt gcaagccggg gatgtactca
     3841 tcggtctgtc ttcgaccggg ctgcacacca acggctattc cctggcccgg aagcttttct
     3901 tctcggtcgc gcattacacg cccgattctt atgtcaacga gatcaagggc aaggtcggca
     3961 ccgagttgat gaaaacccac aagagctact ggccgatcat gcggcgcatg atcgaagccg
     4021 atgcggtttc cgcgatggcc cacatcaccg gcggcggcat cacggaaaac ctgccacgcg
     4081 ctttgcccaa gggcgtcggc gcgatcgtcg agatcaactc ctggccggtg ccgcccatct
     4141 tcgaacacct gcaaaagatc ggtaacgttc cccaagacga gatgctgcgc accttcaaca
     4201 tggggatcgg catgatcctg gtcgtgcccg cgaacaagtt taagaaggtg cagaccatac
     4261 tggagcgttg cggcgagaaa ggccacacca tcggccgcat cgtgaagggc gaaaaaagag
     4321 ttacctacca gtaggcatgt ggctgcccgg actttgccgg cgaaatggcg atttcctgaa
     4381 tagttattcg aaatcgatac tgcattagct tcctcaacgg ctccttccgg attgccgacg
     4441 accgggagct gacgcgtcgt agctcgaaaa ggactggcac ccaatccagt atgttggcct
     4501 cataaggccg acgaatgcgg cgcgaccaca aaccgatcat tcggcaactg cggcagtctt
     4561 ttctaatcga agaatgagcc tgaaggaagc ctgagcagat ggctgaggaa agctcctttc
     4621 agggtttgaa tttaaaaaac agtctagcag tcgtcgaggc ggtgggaatg ggggaatcgc
     4681 gcagcgattt
//
LOCUS       DATMGX010000088         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_220514, whole genome shotgun sequence.
ACCESSION   DATMGX010000088 DATMGX010000000
VERSION     DATMGX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220514"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..692)
                     /locus_tag="VNR65_01470"
     CDS             complement(96..692)
                     /locus_tag="VNR65_01470"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ17372.1"
                     /translation="MQLPRGRPRSFDTEAAVERAMGVFWSRGYHATALPDLLRATKLS
                     RGSLYAAFGDKHSLFLRALDRYVANALTRMDIELDPRREPVDGLRAYLAGYVDRTSGA
                     NGRRGCLLVATAMELAGHDAEVDRRIGSFFKAMEARVAGALSRAKAAGKLADGVEPSS
                     AARILVCFVEGLRVVGKTAPTRITSQATVDALLDRFIR"
     gene            complement(1672..>2066)
                     /locus_tag="VNR65_01475"
     CDS             complement(1672..>2066)
                     /locus_tag="VNR65_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWJ17373.1"
                     /translation="KYARERKPFGRALAENQAIQWPLVELATQAEMQRLLIRKTAWEM
                     DKLTQAQVEHTLSDRVSMCNFWANRLCCEAADRAMQVHGGMGYSRHKPFEHIYRHHRR
                     YRITEGSEEIQKRKVAGFLFGYMGAGKH"
BASE COUNT          390 a          632 c          637 g          407 t
ORIGIN      
        1 cgcagacgat ctggatggcg gacatggcat ctttcctttc aagaccattc ggtctagata
       61 tagacgcgct aacagcggag gcgtcgatgg gttgtctacc taatgaagcg gtcgagaaga
      121 gcgtcaacgg tggcttgcga cgtgatccgt gtcggtgccg ttttaccgac cacgcgcagc
      181 ccctcgacga aacagactag aattcgggcg gcacttgaag gctcaacacc atcggccaac
      241 ttgcccgccg ccttcgcgcg ggaaagtgca ccagccaccc tagcctccat ggctttgaaa
      301 aagcttccga tgcgacgatc aacttcagca tcatggccag ccagttccat ggctgtggcc
      361 accagcaggc atccgcgccg accattagca ccgctcgtgc ggtcaacgta gccggcaagg
      421 taggcccgca ggccgtcgac cggctctctg cgggggtcga gttcgatatc catccgtgtc
      481 agggcattgg cgacgtagcg atcaagcgca cgcaagaaga gcgagtgctt gtcaccgaaa
      541 gcggcgtaaa ggctgccacg cgagagcttc gtcgcacgaa gaaggtccgg tagcgccgtc
      601 gcgtgatagc cgcgcgacca gaacacaccc atcgcgcgct cgacagcggc ttccgtgtcg
      661 aaacttcggg ggcggccacg gggcaactgc actggggttt ggtggctcat tgctgacata
      721 tagaccaatt ggtctttaat ttccagtggc cgctttgggt caaaaacggt cctgtaggga
      781 ccaaaatccg acttccgtta tcccgtagaa gcggacgttg ctcgtcagcc atattcagct
      841 tgaggcacgg ctcggccctt acccctgccg cggttcggat tgcactcgtc aaccgcgtct
      901 tgccacgcga cgatttcgtc ctcaaaccag acccggaggc tcgcggacgc cggcgactgc
      961 tggatactct gctttcgcgg aatcgcggct gtggttgaac aggcatttcg cgttggcaac
     1021 cggggtgcag ggagtcctcg gtgttcctgc cttcacgccc gggcacggtg cggactccga
     1081 cggctgcgac aaattaaccc gacgggtaga atttcgcttt catcgtcgcg caaatcacct
     1141 tcaggagtcc tcccatcctg tccccgagga aggggcgttg gccatcgtca ccgaacgctg
     1201 ggacgggatg cggtggacgc ggcggcatcg tgcgcgcgat gggatcgcag ggcgggataa
     1261 actccgtgag cgatcacagg acgtgccgac gagcggtgcc gaggcgtacg gcgaagtcgt
     1321 gtggttccga tgcctcagtg gttggcgtca agtctttcgg aggcgcaaga gcccaaccgg
     1381 gccaaagcgc cggtttccgg gaggcgacgg aggcaagaaa gcccgatact ccgggaagag
     1441 cgcgatataa gtcgtaaagc cattgcgcag ggaatgtcgg atcgcctccg ctgccctgta
     1501 tgctcgtgtg cgattttctt tttgcccttt gcacacggga ccgcgggtgc agcgtgcatc
     1561 cggcattccc tgctccctcg ttctttcgag ggacaacgaa acgcacacct cgggcgtatc
     1621 ccgccgcgag aacgcgcacg tgtatctgct atttgaaaag tgagacgccc tctagtgctt
     1681 gcccgcgccc atatagccga acaagaaccc cgcgaccttg cgcttctgga tttcctcgct
     1741 gccttcggtg atgcggtagc ggcggtgatg gcggtagatg tgttcgaacg gcttgtggcg
     1801 cgaatagccc atgccgccat ggacctgcat ggcgcggtcg gcggcctcgc agcacagccg
     1861 gttcgcccag aaattgcaca tcgagacgcg gtccgacaac gtgtgctcga cttgcgcttg
     1921 cgtgagcttg tccatctccc acgcggtctt gcggatcaac agccgctgca tttccgcctg
     1981 cgtggcaagt tccaccagcg gccactggat cgcctggttc tcggccagcg cccggccgaa
     2041 cggcttgcgc tcgcgggcat atttta
//
LOCUS       DATMGX010000089         3527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_17264, whole genome shotgun sequence.
ACCESSION   DATMGX010000089 DATMGX010000000
VERSION     DATMGX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17264"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1359
                     /locus_tag="VNR65_01480"
     CDS             208..1359
                     /locus_tag="VNR65_01480"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HWJ17374.1"
                     /translation="MIDRMLPDMAEATRLTRLGKLMEATALIQRVLRGERATADGPTT
                     IDAEYTVVEVEPQDEKPAPRPKIHHEFRPHASIKETVERVGALSKFFREGPHPAPEVP
                     EGASFTTATYTNAAGSREYRLYVPANRSDEQPALIVMLHGCTQTPDDFAAGTRMNALA
                     EEHGFLVAYPAQTSRANMRQCWNWFRPEDQRRGGGEPSLIAGITEEIICQHGVDPRRV
                     FIAGLSAGGAAAAIMGDVYPDLYAGVCVHSGLPSAAAQDMASAFDAMRLGAKVQEPSA
                     RLIPTIIFHGEKDMIVNPSNGEAVAAKAVGSASGLRRETEQGRTAGGRNYVRTNYADE
                     KGRTLCEYWRIRESGHAWSGGCPSGSHTDPLGPDASSEMVRFFFQHHEG"
     gene            1457..1792
                     /locus_tag="VNR65_01485"
     CDS             1457..1792
                     /locus_tag="VNR65_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17375.1"
                     /translation="MPTLTILPSGAAVDASDGDTVLFALLMGGAPISHDCTGKAQCDG
                     CHILIHQGGENLSKIAGPENDRLKQIAGAGPTSRLACQAVVRSGDVTIEVLSTENPGA
                     EEARELRAW"
     gene            complement(2021..>3527)
                     /locus_tag="VNR65_01490"
     CDS             complement(2021..>3527)
                     /locus_tag="VNR65_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWJ17376.1"
                     /translation="PSPQGQPGNAKGNQQPAPIQVEVQKSRSAALEYLQELLSPIIEP
                     ISMGGLLVLFLVFILFYREDLRDRMLRLGGTRDLQTTTAAMNDAGKRLSRYFLIQATI
                     NGCFGLIIGTTLWIIGVPNFVLWGMLAAILRFVPYVGTPISAVFPLVLASAIDPGWSK
                     VLETALLFLAAELVTGQAIEPVLQGQQTGLSPVAIVLAQLFWTLIWGIPGLLLAVPIT
                     VCIAVLGRHVEALSFLGVVLGDEPALAPHEGFYQRVLAGDAIEAAYQAESQLETQRLS
                     DYYDTVPMKALALAEADAARGKLPDDRQADLLEVIEDVVEDLESHTDEEPKSEQAKQA
                     VSKKNGASEKASSSNTEKESSPVLLIQAQGAVDQAASVLLADVLKKRGVTPSVVPYEQ
                     TRRAANSSALGTDARIICISSFEGPQSGSLPVRYLIRRWRRVLPDARFLACFWQPDQD
                     AGKLDELRKKVGADLIATSLKDAAEICCDEMKATLSKKGRNAILGRSAVAG"
BASE COUNT          712 a         1079 c         1066 g          670 t
ORIGIN      
        1 gctcgacatt cgcgtgcttg gcctcgccag catcgcggat gatgtttcgc ccgacctcgc
       61 tcgtgcaacg attgcatcgc ttgccgtgct gggagctctt catgcaagct cagccgtcaa
      121 ggcggcgctt gccgatcgca cgcaataggg tttgccgtga gaattcgccc cagttggttc
      181 ttcgcccgca aaagaaagcg cccgactatg atcgatagaa tgctccccga catggcggag
      241 gcgacccgtc tcacccggct cggcaagctc atggaggcga cggcgctcat tcagcgcgtg
      301 ctccggggcg aacgcgctac cgcagacggc cccaccacga tcgacgccga atacaccgtt
      361 gtcgaagtcg agccgcaaga cgaaaagccg gcgccacggc cgaaaattca ccacgagttc
      421 aggccgcacg cgagcatcaa ggagacggtt gagcgcgtcg gggcattgtc caagttcttc
      481 cgggagggtc cccatccagc cccggaagta ccggaagggg cctcgtttac gacggcgacc
      541 tataccaatg ctgccggctc gcgcgaatac aggctctacg ttcccgccaa ccgcagtgat
      601 gaacagcccg cgctgatcgt gatgctgcat ggctgcaccc aaacgccgga cgacttcgcg
      661 gccgggacgc gcatgaacgc gctcgccgag gagcacggct tcctcgttgc ctatcccgca
      721 cagacttcac gagcgaatat gcggcagtgc tggaactggt tcaggccgga agatcagcgc
      781 cgcggaggag gtgagccatc gctcatcgcg gggatcaccg aggagatcat ctgccagcat
      841 ggcgtagatc cgaggcgcgt cttcatcgca gggctttccg caggcggggc ggcggctgcg
      901 atcatgggcg acgtctatcc cgatctctac gccggtgttt gcgtgcattc gggtctgcca
      961 tcggctgccg ctcaggatat ggcgtctgcc tttgacgcca tgcgcctcgg ggcgaaggtc
     1021 caggagccgt cggcgcggct tattccgacg atcatttttc acggcgagaa ggatatgatt
     1081 gtcaatccat ccaacggtga ggctgtggca gccaaggcgg tgggaagcgc gagcgggttg
     1141 cggcgagaaa cggaacaagg ccggaccgca ggcggccgta actacgtgcg taccaactac
     1201 gccgatgaaa aggggcgcac cctgtgcgaa tactggcgca tccgcgagag cggccatgca
     1261 tggtcgggcg gctgcccgtc aggctctcat accgatcccc tcggtcccga tgcttcaagt
     1321 gaaatggtcc gtttcttctt ccagcatcac gagggctgag cccgctaggc aaaagcttct
     1381 cgcttcgcca ttgtcattac cttccgtcct gtgtcatgac cactttccat caagatagat
     1441 ttggggactg aacgcgatgc cgacactgac gattctgcct tcgggcgcag ccgtggacgc
     1501 ctctgatggc gacactgtac tctttgcact tctcatggga ggcgccccta tcagccacga
     1561 ctgcaccgga aaggcgcagt gcgatgggtg ccacatcctc attcatcaag gcggagagaa
     1621 cctctcgaag atcgcaggcc ccgagaatga ccgcctcaaa cagatcgctg gcgcaggccc
     1681 aacgtcccgg ctggcctgcc aggcggtcgt cagatccggt gatgtaacga tcgaagtttt
     1741 gagcaccgaa aacccaggag cagaggaagc gcgggaactg agggcatggt gaaccctaag
     1801 ggacggcctc tgcctgctcg ttcacgcaat gtgagggggc ccggccgtgg acttggtcct
     1861 ctcttccaga catcaatctg agcagagcag cgtgtggctc cgaacttaga tgtgcaaaat
     1921 tagaaggctc gcttcgagtg acgggcaagc tcgggcgtga acggcggctc aactccaaca
     1981 gcaggcactc ccgaaatcgg cccgatgtca aaactttgat tcaacctgcc actgcgctcc
     2041 ggccgaggat ggcgttccgg cccttcttgg agagggttgc cttcatctcg tcgcagcaga
     2101 tttcagccgc atccttgaga gaggttgcga tgaggtctgc tccaaccttc ttgcgcaact
     2161 cgtccaactt tccggcatcc tgatccggtt gccagaaaca ggcgagaaat cgcgcatcgg
     2221 gaagcactcg ccgccaccgc cggatcagat atcgcacggg cagcgacccc gattgcgggc
     2281 cctcgaagga cgagatgcaa ataatcctgg cgtcggttcc caaggcactt gagttggccg
     2341 cgcgccttgt ctgttcatag ggcacaacag atggcgtcac cccgcgcttt ttcagaacgt
     2401 ctgcaagcaa cacgctcgcc gcctgatcga cggcgccttg ggcctgaatg aggagcaccg
     2461 ggctactctc cttttcggta ttactggagg acgccttttc cgatgcgcca ttctttttgc
     2521 tcacggcctg tttggcttgt tcggatttag gctcttcgtc cgtatgggat tccaggtcct
     2581 cgacaacgtc ctcgatcacc tcaagaagat ctgcctgcct gtcgtccggc aatttccccc
     2641 gcgccgcatc ggcttccgca agcgccagcg ccttcatggg aaccgtgtcg taatagtcgg
     2701 aaaggcgctg tgtttcgagt tggctctcag cctggtaggc ggcttcgatc gcatcgcccg
     2761 ccagcacgcg ctgataaaag ccctcatgcg gggcgagcgc cggctcatcg cccaagacga
     2821 cgcctaagaa gcttaaagcc tcgacgtgcc tgccaagcac cgcgatgcac accgtgatcg
     2881 gcactgcgag aagcaagcct ggtatgcccc agatcaatgt ccagaacagc tgcgccaata
     2941 cgatcgccac tggcgaaagg ccggtctgct gaccctggag aacaggctct atggcttgcc
     3001 ccgtgacgag ttcggccgcg aggaacaaca gcgcggtttc aaggaccttc gaccagccgg
     3061 gatcgatcgc cgaggccagc accagaggga acacggcgga aatgggcgtg ccgacatagg
     3121 gcacaaatct caggattgcg gccagcatgc cccagaggac gaaattcggt acgccgataa
     3181 tccaaagggt tgttccgatg atgagaccga agcatccatt gatggtggcc tggatcagaa
     3241 aatagcggct gagacgcttt ccggcgtcgt tcatcgccgc cgttgtggtc tgcaggtcgc
     3301 gtgtgcctcc gaggcgcagc atgcggtcac gcaaatcttc ccgataaaac aggatgaaca
     3361 ccaggaacag gacgagaagc ccgcccatgc tgatcggctc gataatgggg ctgagcagtt
     3421 cctgaaggta ctcaagggcg gcggagcgcg atttttgcac ctccacttgt ataggggcgg
     3481 gctgctgatt tcccttggcg ttaccgggct ggccttgggg ggaagga
//
LOCUS       DATMGX010000090         2622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_464385, whole genome shotgun sequence.
ACCESSION   DATMGX010000090 DATMGX010000000
VERSION     DATMGX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464385"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VNR65_01495"
     CDS             complement(<1..263)
                     /locus_tag="VNR65_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ17377.1"
                     /translation="MDLSQLEVFLTVARERRFSRAAEKLFRTQSAVSQTIRKLELEIG
                     EPLFDRSSRDGVLTDAGRVLQEYAEKLLNLRQDAQTALVEMRE"
     gene            545..1888
                     /locus_tag="VNR65_01500"
     CDS             545..1888
                     /locus_tag="VNR65_01500"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWJ17378.1"
                     /translation="MDRPRITIFDTTLRDGEQSPGCSMNVQEKLRLAQQLDRLGVDVI
                     EAGFPIASDGDFEAVQRIAASVRRPIIAGLARACIGDVDRAWQALRVAAHPRIHVFLA
                     TSDIHLKHKLRITREQCLEQAREAVRHAKSLCADVEFSPEDATRTDVAFLCQVVEAAI
                     AAGATTVNIPDTVGYSMPEEYGQLIRTLREKVAGIDKVAISTHCHNDLGLAVANSLAG
                     VQHGARQVECTINGIGERAGNASLEEIVMVMRVRRDRYAYETAVAGEHLYPSSQMLAE
                     ITRVPVQPNKAITGRNAFAHEAGIHQDGMLKNPLTYEIMTPQSVGVPDSRLVLGKHSG
                     RHALALRCEQLGHQFERRELDEVYRKFVVLADRIKKVRDSHLLELIQDVISSDAGGKK
                     FPPATAPLPFPHAAAAGANSNGSHPAHVSGSAGQATVSIPFADHHSEQEDYLWGV"
     gene            2006..>2622
                     /locus_tag="VNR65_01505"
     CDS             2006..>2622
                     /locus_tag="VNR65_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWJ17379.1"
                     /translation="MQLRVTSLPGDGIGPEVTSQAICVLEEVADGFGHDLELDEKEIG
                     GVALTKFGNPLPDTTIQSCLASQAVLLGAVGNPAFDHNPRELRPEAGLLRLRQELGAF
                     ANLRPAVFYPALAGCSPLREEIVRGTDILIVRELLGGLYFGEPRSIEGAPGTRQAINT
                     MRYSEPEIERIARIAFQLARTRRGRLLSVDKSNVLECSRLWREVVS"
BASE COUNT          543 a          815 c          742 g          522 t
ORIGIN      
        1 aactcgcgca tctccaccag cgccgtctgt gcgtcttgcc gcaggttgag caacttctcg
       61 gcgtattcct gcaaaacacg tcccgcgtcg gtgaggacgc cgtcccgcga agaacggtcg
      121 aacagcggct cgccgatctc cagttcaagt ttccggatgg tctggctaac cgccgattgc
      181 gtgcggaaca gtttttcagc ggcccgcgaa aaccgtcgtt cgcgcgccac ggtgaggaag
      241 acttcgagtt gcgaaaggtc catttgtcac cccttactgt cgagacgggt cttgccccgt
      301 ctcattctgc ggacaggaga cacggcaagg cgcgtctcta cgagaaagca atcggtcctt
      361 ggagtgccgg gcatcggcac cccaattttg gcccgggcca acccactttt atcaatcgta
      421 gatacttcat aagttttact aatagtatcg cgcacactaa tggtccggta aacataataa
      481 tcttttcttt cgccccaatc cgccgataga gtagagagat acatttttcg gaggtacgga
      541 atccatggat cggccgcgca tcaccatctt cgacaccacg cttcgtgacg gcgaacagtc
      601 gccgggctgc agtatgaatg tgcaggaaaa gctgcgtctg gcccagcaac tcgaccgcct
      661 cggcgtggac gtgatcgagg ctggattccc gatcgcttcc gatggcgact ttgaagccgt
      721 gcaacgcatt gccgcctcgg tgcggcgtcc catcatcgct ggcctggctc gggcttgcat
      781 cggcgacgtc gaccgcgcct ggcaggcgct gcgggtcgcg gcgcacccgc gcatccacgt
      841 ttttctcgca acctctgata ttcatctgaa acacaagctg cgcatcacgc gggagcagtg
      901 tctggagcag gcgcgggaag cggttcgtca cgcgaagtca ctctgtgccg acgtggaatt
      961 ctcgcccgaa gatgccaccc gcaccgatgt ggcgtttctc tgccaggttg tcgaagcggc
     1021 gatcgcagcg ggcgcaacca ccgtcaacat tcccgatacc gtcggttaca gcatgcccga
     1081 ggaatacggc caactcatcc gcacgctgcg tgagaaagtc gccggcatcg acaaagtagc
     1141 gatctccacg cactgccaca acgacctcgg tctggcggtc gcgaactcac ttgcaggagt
     1201 gcagcacgga gcgcgccaag tggagtgcac catcaacggg attggagagc gcgccggcaa
     1261 cgcttctctc gaagaaatcg tcatggtcat gcgggtgcgc cgcgatcgct atgcttacga
     1321 aaccgcagtc gctggcgagc acctgtatcc gtcaagccag atgttggcgg agatcacgcg
     1381 ggttccggtg cagcccaaca aggcaatcac cggccgcaat gcgtttgcgc acgaggctgg
     1441 aattcaccaa gatggcatgc tcaagaatcc gctgacctac gaaatcatga cgccgcagtc
     1501 ggtgggcgtg ccagattcgc ggctggtact gggcaagcac tccggccggc acgcgctggc
     1561 tctgcgctgc gaacaactgg gacaccagtt tgagcgccgc gaacttgatg aggtctatcg
     1621 caagtttgta gttcttgccg accgcatcaa aaaagttcgg gacagccatt tgctcgaact
     1681 gatccaggac gtcatcagca gcgatgccgg cggcaaaaag tttccgcccg caaccgcccc
     1741 gctgcctttc ccgcacgccg cagccgcggg agcaaattcc aatggctcgc atcccgctca
     1801 cgtttccggc agcgccggcc aagcgacggt gtcgattccg ttcgcagacc atcacagcga
     1861 gcaagaagac tacctctggg gtgtgtaaca acgggatagg aaccaacaca gccgacagta
     1921 aaaaagtgtg aaatgcctcg aaacaggcta cccgtgcggc gattcctcct atattctttc
     1981 ggtaccgaaa cgaatcgagg attccatgca actccgcgtc acctccctcc ccggcgacgg
     2041 aattggtcct gaagttacat cccaagccat ttgtgttctg gaagaggtgg ccgacggatt
     2101 tggccacgat ctcgaactcg acgaaaaaga aatcggtgga gttgctctga caaagttcgg
     2161 taatcctttg ccggacacaa caattcaatc ctgcctggct tctcaggcgg tgctgctcgg
     2221 ggccgtcggc aatcccgcct tcgatcacaa tcctcgcgaa cttcgccccg aagctggact
     2281 gttacgcctc cgccaggaac ttggagcgtt cgccaatctt cgtccggcgg ttttttatcc
     2341 ggcgctcgcg ggctgctcgc cgctgcgcga agaaatcgtc agaggaacgg atattctcat
     2401 tgtgcgcgaa ctgctcggtg gattgtattt cggcgagccg cgctcgattg aaggcgctcc
     2461 cggcacgcgc caagcgatca acaccatgcg ctacagtgaa ccggagatcg agcgcatcgc
     2521 ccgcatcgcc tttcagctcg ctcgaacccg ccgcggccgc ctgctctcgg tcgacaagtc
     2581 gaatgtcctg gagtgctcgc gtctctggcg tgaagtcgtc tc
//
LOCUS       DATMGX010000091         2299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_251917, whole genome shotgun sequence.
ACCESSION   DATMGX010000091 DATMGX010000000
VERSION     DATMGX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251917"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..>2299
                     /gene="dnaE"
                     /locus_tag="VNR65_01510"
     CDS             179..>2299
                     /gene="dnaE"
                     /locus_tag="VNR65_01510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWJ17380.1"
                     /translation="MTKPKFIHLNVHSAYSLLEGALPVKALAKLAAADGQPALAITDR
                     NNLFGALEFSEALAAEGVQPIVGCTLTVAFPQASDESPRPGARAAHHGSIVLLAKNAA
                     GYLNLMRLSSQAFLDHAAEGAPHVALAQLEECAEGLIALSGGPDGPADKAYAAGNASL
                     AESRLTALAAIFSERFYIEIQRHGLRRERAAEPQLLRWAYDNGVPLVATNEAYFKKRD
                     VFEAHDALLCIAEGRYVAEDDRRRLSPEHYLKPQSEMAALFADLPEAIENTVEIAKRC
                     AFRPVPRKPILPSFVPGAEDTAAAEAAELRAQAEAGLARRLAAIPEHLRGASEEEYKK
                     RLAFELDVITRMKFPGYFLIVSDFIKWTKAQGIPVGPGRGSGAGSVVAWSLTITDLDP
                     MRFGLLFERFLNPERVSMPDFDIDFCQDRRDEVIAYVRQKYGADRVAQIITFGKLQAR
                     AVLRDVGRVLQMPYGQVDRLCKMVPNNPANPVTLAQAIESEPGLQEARDREPIVKQLL
                     NTAQQLEGLYRHASTHAAGVVIGDRPLVELVPLYRDPRSDMLVTQFNMKWVEQAGLVK
                     FDFLGLKTLTVLQKACALLKKAGRPIELEALPLDDTATYELIAKGDTGGVFQLESTGM
                     REALRKLKPDRFGDIVAINALYRPGPMDNIETFINRKHGIEEPDYLHPLAKPILEETY
                     GVIIYQEQVMQLAQVLSGYSLGEAD"
BASE COUNT          447 a          742 c          698 g          412 t
ORIGIN      
        1 tgaaggacga cttatcgagg tgagccagaa gcccggcacg gccaaacccg aagcggctta
       61 cgcggtaccc ccttcattcg gctagggaaa gcgattggga taactcgatg ctatccctgc
      121 gaatcgctgt tgcgcacgct aatcttcggc ttcggctcaa acgaccctgc gggagctgat
      181 gacgaagccc aaatttatcc atcttaacgt ccattccgcc tattcgctgc tggaaggcgc
      241 gctgcccgtc aaggcgttgg caaaactggc cgctgccgat ggccagcctg cgctcgccat
      301 caccgacagg aacaatcttt tcggcgcgct cgaattttcg gaggcgctcg ccgccgaagg
      361 ggttcagccg atcgtcggat gcacgctgac ggtcgctttt ccgcaagcct ccgacgagag
      421 cccccgcccc ggagcgcgcg cggcccatca cggttccata gttttgctgg cgaagaacgc
      481 agccgggtac ttgaacctga tgcgcctttc cagccaggct ttcctcgatc acgcggctga
      541 gggcgcgccg catgtagcgc tcgcgcagct cgaagaatgc gcggagggcc tgattgcgct
      601 gtcaggcggc cccgatggcc cggcggacaa agcatacgct gccggtaacg cctctttagc
      661 cgaatcacga ctcacagcgc tggccgccat tttcagcgag cgattttata tcgagatcca
      721 gcggcacggt ttgcgtcgcg agcgcgcggc tgagccccag ctcttgcgtt gggcttatga
      781 caatggcgtg ccgctcgtcg ccaccaacga agcctatttc aagaagcgcg atgttttcga
      841 agcccacgac gcgcttctct gcatcgcgga aggacgctac gtcgcggaag acgaccggcg
      901 ccggctctcg cccgagcatt atctcaagcc acaaagcgag atggcggcct tgtttgccga
      961 tctgccggaa gccatcgaga acacggtcga gatcgccaag cgctgcgcct ttcggccggt
     1021 tccgcgcaag ccgatcctgc cgagcttcgt ccccggcgct gaagacaccg cagccgccga
     1081 agccgccgaa ctgagggcac aggcggaagc ggggctcgcc cgccgtctag ccgccatccc
     1141 ggagcatctc cgcggggcga gcgaggagga atacaagaag cgcctcgcct tcgaactcga
     1201 tgtcatcacc cgcatgaagt ttccgggcta cttcctgatc gtgtccgact ttatcaaatg
     1261 gacgaaggcg cagggcatcc ccgtggggcc gggccgtgga tcgggcgctg gctcagtcgt
     1321 ggcgtggtcg ttgacaatta ccgaccttga cccgatgcgc ttcgggcttc tcttcgagcg
     1381 cttcctcaac cccgagcgcg tctcgatgcc ggacttcgac atcgacttct gccaggaccg
     1441 gcgcgacgag gtgatcgcct atgtccgcca gaagtacggc gcggaccggg tcgcgcagat
     1501 catcaccttc ggaaagctgc aagcacgcgc cgtgctccgc gacgtgggcc gcgttttgca
     1561 aatgccctat gggcaagtcg accggctctg caagatggtg ccgaacaacc cggcgaaccc
     1621 ggtgacgctc gcccaggcca tcgaaagcga gcccgggctg caggaggccc gcgaccgcga
     1681 gccaatcgtg aagcagcttc tcaacacggc gcaacagctg gaggggcttt accgccacgc
     1741 ctccactcac gccgcgggtg tggtcatcgg cgatcggccg ctggtggaac tggtcccgct
     1801 ctaccgtgat ccgcgctcag atatgctcgt aacccagttc aatatgaaat gggtcgagca
     1861 ggctgggctt gtgaagttcg acttcctggg tctaaagacg ctaacagtgc tgcagaaggc
     1921 gtgcgcgctt ttgaaaaagg cgggcaggcc catcgaactc gaggcactgc cgctcgacga
     1981 cacggccact tacgagctga ttgcgaaggg cgataccggc ggcgtgttcc agcttgaaag
     2041 tacgggcatg cgcgaggcgc tccgcaagct gaagcccgac cgcttcggcg acatcgtcgc
     2101 tataaacgcg ctttatcgtc ctggcccgat ggacaacatc gagaccttca tcaaccgcaa
     2161 gcacggtatc gaagaaccgg attacctgca cccgctcgcc aagccgattc tggaagagac
     2221 ctatggcgtc atcatctacc aggagcaggt gatgcagctc gcgcaggtgc tgtcaggcta
     2281 cagcctcggc gaagctgac
//
LOCUS       DATMGX010000092         3018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_152534, whole genome shotgun sequence.
ACCESSION   DATMGX010000092 DATMGX010000000
VERSION     DATMGX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152534"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VNR65_01515"
     CDS             <1..464
                     /locus_tag="VNR65_01515"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HWJ17381.1"
                     /translation="PPKLAQATVRNAVAEALRLQPDIPLIAGGKSFGGRMTSQAQALE
                     PLPAVHGLAFLGFPLHPAGRPSIDRADHLFKVHVPMLFLQGTRDALAQTGELEPLCQA
                     LGAHATLKLFPDADHSFHVPARSGRTDAQVLAEMLDALAAWAAGLEKGGAV"
     gene            861..1919
                     /locus_tag="VNR65_01520"
     CDS             861..1919
                     /locus_tag="VNR65_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17382.1"
                     /translation="MQNSLEDLGSSVPKVNGVVLASRYGKDNNLSNHGHPDDQALIHR
                     DDQKWGRSFETGLTAGKRSRKLFPSILSYLAIAILSGGISGGALLYFLLHYAVPHDTE
                     TSTALSSAPGVGEGETSDRPVIERSLSFTPVSKAVSLPQDGVERQNAPTGNAGGPSLP
                     AELNRKAVEVMDPTGILPATTGPDAKEPDAGSKTFASTGADAKQPDIRRKTAAIAPVE
                     EPTPAAPMPALVPAPKDLQEGRPPASDKADPGVTKRDTQLPKLSAEQEDKMLKRASTL
                     LGENDIAGARLIFHYLANHGSAGGAFALAESYDPKKWGSHRVTGMAPDGDLARNWYAR
                     AAELGSREAVAILRKEKP"
     gene            1962..>3018
                     /locus_tag="VNR65_01525"
     CDS             1962..>3018
                     /locus_tag="VNR65_01525"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWJ17383.1"
                     /translation="MRRSSPEGVIPQATHWLASILVASLCLFVCLDTASAQTQFKTTT
                     YEQKKREANDISVAIINSGLSCTCARFSEDMRNVINDLRPDGIRVLPVLGVGGLQNLK
                     DVLFLKGIDMGIVDSDNLALLKKKDPKLYADIEQRVQYITKLYNAELHVLARNDIKSL
                     ADLKGKRVNFNVKEHQTEVTGDHLFAMLNIPVEKTNYDVEESIRRLLSGDLAAIMIVT
                     GAPQSALAKLKKEDGLHFVPVDQDSLPGTDFTPVIAEYLPAEFTSKTYPNLVPEGTSV
                     PTLANRALLVTYGWPEGSVRYNRVTKFVNELFGKIDEFHSPSRHPKWAEINLWADIPG
                     WTRFKPASQWLASHQRMA"
BASE COUNT          690 a          851 c          828 g          649 t
ORIGIN      
        1 atccgccgaa gcttgcccag gctaccgttc ggaacgctgt ggcagaggcg ttgcgtttgc
       61 aaccggacat cccgcttatc gccggcggca aatccttcgg cggccgcatg acatcgcagg
      121 cgcaagccct ggagccgctt cctgccgtcc acgggctcgc attccttgga tttccgctgc
      181 atccagccgg gcggccctcg atagaccgcg ccgaccattt attcaaagtg cacgtcccca
      241 tgctgtttct gcaggggacg cgtgacgctc tggcccaaac gggcgaactc gagccgcttt
      301 gccaggcgct cggcgcgcac gcgaccttga agctcttccc agacgctgac cattcctttc
      361 atgtcccggc ccggagcggg cgtaccgatg cgcaggtgct ggccgagatg ctggatgcgc
      421 tggccgcgtg ggccgccggg ttagaaaaag gcggcgctgt ctaagcaact taccctcctc
      481 aaaaggccat gtatacgttt gcgtataagc ttgtcgacag ttttggcgaa acattttctc
      541 cgaaaaacgg aataactccc ttgcttccgc gtttcattgt cagctaagtt tttcaaacta
      601 tagcactaga atcagacagt tttttctata caaccagtgg ttgcttttgg ctatgtgtgc
      661 aaggcttgtc caatgcaact atataaatgc tataataacg aggctttaat gtcataaata
      721 taccacgatc cagataatat gattatccgc ttggtgagga tctcatgaca agcagtgaac
      781 tgagtaaaat aaaacgccaa cgcatcgcag agtcgctgtc ttgtggggtg accgaaaaca
      841 aatccacgct tgaagcatat ttgcaaaata gcctggaaga tctgggatcc tctgttccca
      901 aggtgaacgg ggtcgtgctt gcctcgcgtt acggcaaaga taacaatctt tctaatcatg
      961 gtcacccaga cgatcaggcg ctgatacacc gcgacgatca gaaatggggc cgtagcttcg
     1021 aaacaggttt gactgccgga aagcgttcga gaaagctctt cccatccatt ttgtcgtatt
     1081 tggcgattgc tatactatct ggagggatat cgggaggtgc gcttctctac ttcctgttgc
     1141 attacgcggt tcctcatgac actgaaacca gcactgctct ttcatctgcg cctggggtag
     1201 gggaaggcga aacttcagat cgcccggtca ttgagcgatc gctctcgttt acccctgtct
     1261 ccaaagcagt gtccttgcca caagacggcg ttgagcgcca gaacgccccg acaggaaatg
     1321 cgggcggccc atcccttcca gccgagttga acagaaaagc tgtcgaagtc atggatccca
     1381 cggggatcct tccggccact accggcccgg atgcgaagga gcctgatgcc gggagcaaga
     1441 catttgcctc tacgggcgcg gacgcgaagc agcctgacat caggagaaag acagctgcca
     1501 tcgcccctgt agaggagcca acgccggccg cgccaatgcc tgcgctggtt cctgctccga
     1561 aagatcttca ggaagggaga cctcccgcaa gtgacaaggc cgatccgggc gtcacgaaac
     1621 gggatacgca gcttccgaaa ctctcagcag agcaagagga taagatgctg aaacgggcat
     1681 ccaccctcct cggcgagaat gacatcgcag gtgcgcggtt gattttccac tatctggcga
     1741 accacggaag cgccggcggc gcgtttgctc tcgccgagag ctacgacccg aagaaatggg
     1801 gcagccaccg cgttaccgga atggccccgg atggagatct ggctcgcaac tggtatgcgc
     1861 gcgcagccga attgggaagc agggaagctg ttgccatcct gcgcaaagag aaaccctgat
     1921 cctgtagcga agcttcgaat ctcaattcat cgagggttaa catgagacgt tcgtcgcctg
     1981 aaggtgtcat tccgcaagcc acgcactggc tggccagtat tctcgtggcc agtctttgcc
     2041 tctttgtctg cttggataca gcgtcggcgc agacgcagtt caagacgacg acctacgagc
     2101 agaaaaagag agaggccaac gatatttcgg tggcgatcat aaattccggg ctttcgtgca
     2161 cctgcgctcg cttttctgaa gatatgcgca atgtcatcaa tgacctcagg ccggatggga
     2221 tacgcgtttt gcccgttctc ggtgtcgggg gcttgcagaa cctgaaagac gtcctcttcc
     2281 tgaagggcat cgacatgggc attgtcgact cggataacct ggccctcctt aaaaagaagg
     2341 atcctaagct ctacgccgac atcgaacagc gcgtgcagta tattacgaag ttgtacaacg
     2401 ccgagcttca cgtgcttgcc cgaaacgaca tcaagtctct cgcagacctc aagggcaaga
     2461 gggtcaactt caacgtcaag gaacatcaaa cggaggttac gggcgaccac ctctttgcga
     2521 tgctgaacat ccccgtcgag aagacgaact acgatgtcga ggaatcgatc aggcggctgt
     2581 tgagcggtga tctggcggcc atcatgatcg tgacgggcgc tccgcagtcg gcgctggcca
     2641 agctcaagaa agaggacggc ttgcactttg ttcctgtcga tcaggatagc ctgcctggca
     2701 cagattttac gcccgtgatc gcggaatatc tgccggccga gtttacgagc aaaacctatc
     2761 ccaatctcgt gccggaaggg acgtcggttc cgacacttgc caaccgcgct cttcttgtta
     2821 cctatggctg gccggaaggc tctgtgcgtt acaacagggt cacgaagttc gtgaacgagt
     2881 tgtttggcaa gatcgacgag ttccattcgc cttccagaca tcccaaatgg gccgaaatca
     2941 atctttgggc cgatatcccc ggctggacga gattcaagcc tgcttcccag tggcttgcct
     3001 ctcaccaaag gatggcgc
//
LOCUS       DATMGX010000093         3745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_464152, whole genome shotgun sequence.
ACCESSION   DATMGX010000093 DATMGX010000000
VERSION     DATMGX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464152"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1085)
                     /locus_tag="VNR65_01530"
     CDS             complement(78..1085)
                     /locus_tag="VNR65_01530"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWJ17384.1"
                     /translation="MSSLLSRYAESVFWMARYFERAESLSRVIETQTSFQRGRQDNSW
                     AWIVALYADEKAFAKAFSEPSAENVIRYYMAYIDNPGSIQSSIRSARENARALRPMLS
                     TDMWYQLNDFYNRLFAFAPNDFSEVRLSRTCDAIKRGCYAQIGVAESTLYHDETWPFF
                     KLGLYLERADQTSRLLDVRFAQQATGMARDEGLYDFTFWTILLRAASAYHAYRRVFPH
                     YIDPSEVARFLIFDPRLPRSLAFCVGEIQAMIEFLRRNCGLKNAAGAAEQAEKLIEGL
                     EAASKDERLLENLHTFNDWVQLSLIHIAEELGQAFFGYAQTPEVARAPSQTQSQTMAA
                     R"
     gene            complement(1082..2563)
                     /locus_tag="VNR65_01535"
     CDS             complement(1082..2563)
                     /locus_tag="VNR65_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWJ17385.1"
                     /translation="MATITALKTPSTVAPLDNYNPGSRYCELIGSPENRNRHLTALWH
                     RLQQLELQQLRERSADAERELFTLGITFTVYSDRTAIDRILPFDVIPRVITAADWRTI
                     DRGVKQRIKALNLFLWDVYHDRKILKDGLVPSDLVLGNSNYQPEMVGYNPPCGTYIHI
                     GGTDIIRDPAGDFFVLEDNARTPSGVSYVVENRHLMQRAFPDLMQGFAIESVSEYGTQ
                     LGNKLLEVAPSGISDPQIVLLSPGVFNSAYFEHVFLSREIGIPLVEGRDLFLEDDKVF
                     MKTVSGPKRVHVIYRRINDDFLDPEVFKPESVLGVKGLMRAVRKGNVTLANAVGTGVA
                     DDKAVYAYLPRIIKYYLDEEPILPNVETHICREPDALAYTLNHLNELVVKPVGESGGY
                     GICIGPKATKAELDLCRDQLKANPSNYISQPMIELSVCPTLCDEGVGPRHVDLRPFAV
                     TGEDTWLLPGGLTRVALRKGSIVVNSSQGGGTKDTWVLKEDDA"
     gene            complement(2757..>3745)
                     /locus_tag="VNR65_01540"
     CDS             complement(2757..>3745)
                     /locus_tag="VNR65_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HWJ17386.1"
                     /translation="TMIQRHKLKEADYRGERFKDWPSDLKGNNDLLVLTQPGIIQGIH
                     EAYFEAGSDIIETNTFNAQTVSMADYGMEALAFEINVAAAKLARAAADKYTALTPEKP
                     RFVAGAIGPTNRTASLSPDVNNPGFRNISFDELVAAYSEQTRGLIEGGVDTILIETVF
                     DTLNAKAAGFAVKETFDAMGIELPLMISGTVTDLSGRNLSGQTPEAFWYSLRHLKPFS
                     IGMNCAFGAEQLRPPIAQISKVADTHVSVYANAGLPNAMGEYDETPESMADAMRAWAA
                     DGLLNIVGGCCGTTPDHIMAIAKAVAPFPPRESPVVEPRLRLSGLEPFVQGP"
BASE COUNT          798 a         1063 c         1120 g          764 t
ORIGIN      
        1 aaagaccttc gtgatggtga ttggatcaaa gggggagcgc ccgccggcat aatcgccggg
       61 cgagagtatt ttggcggtca gcgcgccgcc atcgtctgcg actgcgtttg gctcggcgcg
      121 cgcgccacct ccggcgtctg cgcatagccg aagaaagctt ggcccaactc ttccgcgatg
      181 tgaatgaggc tcagctgcac ccagtcgttg aatgtatgga ggttttcaag cagacgctca
      241 tccttgctgg ctgcttcgag cccctcgatc agtttctcag cctgctccgc ggccccggcg
      301 gcattcttca gcccgcaatt acgccgcagg aactcgatca tcgcctgaat ttctccgaca
      361 cagaaagcaa gagaacgcgg cagacgcgga tcgaaaatca aaaagcgggc aacctcgcta
      421 gggtcgatgt agtgagggaa aacacgccgg taggcgtgat acgcggaagc cgcgcgcaag
      481 agaatggtcc agaaagtgaa gtcgtagagc ccttcgtcgc gcgccatgcc cgtcgcttgc
      541 tgggcaaagc gcacatcgag aagccggctt gtctggtcgg cgcgttcgag atagagcccg
      601 agcttgaaaa agggccaagt ctcgtcgtgg taaagcgtgc tctccgcaac accgatctgc
      661 gcgtagcaac cgcgcttgat cgcgtcgcag gtgcgcgaca ggcggacctc ggagaagtcg
      721 ttcggagcga acgcgaacag ccggttatag aagtcgttca gctgatacca catgtccgtc
      781 gacagcatgg ggcgcagcgc ccgcgcgttt tcgcgagcgg agcggatcga agactggatc
      841 gaacccggat tgtcgatgta agccatgtag tagcggatga cgttttctgc gctcggttcg
      901 gaaaacgctt tcgcgaacgc cttctcatcg gcatagagag cgacgatcca cgcccaggaa
      961 ttgtcctgcc gcccgcgctg gaaggacgtt tgtgtctcta tgacgcggga gaggctttcg
     1021 gcgcgctcga aatagcgcgc catccaaaag acgctttcgg cgtatcgtga gaggagactg
     1081 ctcaagcgtc atcctccttc agaacccagg tatccttcgt tccgccgccc tggctcgaat
     1141 ttacgacgat ggagcctttg cgaagcgcga cgcgggtcag gccgcccggc aggagccacg
     1201 tatcttcgcc cgtcactgcg aaaggccgga gatcgacgtg ccgcggccca acgccttcat
     1261 cgcataaggt cggacagacg gaaagctcga tcatgggctg actgatatag ttggatggat
     1321 tcgccttcag ctgatcccgg cagagatcga gttcggcctt cgtcgccttc ggtcctatgc
     1381 agattccgta accgccggac tcgccaacgg gcttcaccac aagctcattc agatggttga
     1441 gcgtgtaggc caaagcatcc ggctcccggc agatatgggt ctcgacattg ggcaggattg
     1501 gctcttcgtc gagatagtat ttgatgatgc gcggaagata ggcgtaaacc gccttgtcgt
     1561 cggcgacgcc cgtgccgacg gcgttggcga gcgtaacatt ccccttacgc acggcgcgca
     1621 tcagaccctt gaccccgagc acgctctctg gcttgaacac ctccgggtcg aggaagtcgt
     1681 cgttgatgcg gcggtagatg acgtggacgc gtttggggcc cgacaccgtc ttcatgaaca
     1741 ctttgtcgtc ttccaggaac aaatcgcggc cttcaacgag cggaataccg atctccctgg
     1801 agaggaaaac gtgctcgaaa taggccgagt tgaacacgcc gggcgaaagc agcacgatct
     1861 gaggatcgga aattcctgac ggcgcaacct ccagaagctt gttcccgagt tgcgtgccgt
     1921 attcggaaac actttcgatc gcgaagccct gcatgagatc ggggaaagcc cgctgcatca
     1981 gatgccggtt ctcgacgaca taagacacgc cggaaggggt ccgcgcattg tcttccagca
     2041 cgaagaaatc gccggcggga tcgcggatga tatcggttcc gccgatgtgg atataggtgc
     2101 cgcaaggcgg gttatagccg accatttcgg gctggtagtt cgaattcccc agcacgagat
     2161 cggacggcac gaggccgtct ttgagaatct tgcggtcgtg atagacatcc caaaggaaca
     2221 gattcagcgc cttgatgcgc tgcttgaccc cgcgatcgat cgtgcgccaa tcggcggctg
     2281 tgatcacgcg ggggatgacg tcgaagggca gaatgcggtc gattgccgtt ctgtcgctat
     2341 agacggtgaa cgtaattcca agggtgaaca gctcgcgctc ggcatccgcg gaccgctcgc
     2401 ggagttgttg aagttcgagt tgctggagac gatgccaaag agcggtcaga tggcgattcc
     2461 ggttctcggg ggaaccgatc agttcgcaat acctggagcc ggggttgtaa ttatcgagag
     2521 gagccaccgt agacggagtc ttcaaagctg tgattgtcgc catgcagccc cctgcagtgc
     2581 atcagccctt catcggcgcg gaccagatgt ggagatttta gagtcaatac aaagaaaaga
     2641 ctaacacagc aacgtactgc gtcggaacat aattttccgc tgaaatattc agcaaaatgc
     2701 ctccaatttt gcctattttt tcagcacagc cagcagaaga acttgccgct gaaaacttac
     2761 ggtccctgca cgaaaggttc aagccccgaa aggcgcaaac gcggctcaac cacaggactc
     2821 tctcgtggcg ggaacggcgc aactgctttc gcgatagcca taatatgatc cggggtggta
     2881 ccgcaacatc cgccgacgat attcagcagg ccatcggctg cccaggcacg cattgcgtca
     2941 gccatggatt ccggcgtctc atcgtactcg cccatggcgt tcggaaggcc tgcgtttgca
     3001 taaacgctga catgcgtgtc ggccaccttc gagatctgcg cgatgggcgg acgcaattgc
     3061 tctgcgccaa aagcacagtt catgccgatg gagaaaggct tcaaatggcg cagcgaatac
     3121 cagaaggctt cgggggtttg gcccgagagg ttgcgacccg agagatccgt gaccgtgcct
     3181 gaaatcatga gcggaagctc gatccccatg gcgtcgaatg tttccttgac cgcaaacccg
     3241 gctgctttcg cattcagcgt gtcgaacacg gtctcgatca ggatcgtatc cacgcccccc
     3301 tcgatcaggc cacgggtctg ctcgctatag gccgcgacca gctcatcgaa ggagatgtta
     3361 cgaaatccgg gattgtttac gtctggcgag agggacgccg tgcggttcgt tggccctatg
     3421 gcgccagcca cgaagcgcgg cttttccggc gtcagtgctg tgtatttgtc cgccgccgct
     3481 cgggcaagtt tcgccgccgc gacattgatc tcgaatgcga gcgcctccat accgtaatct
     3541 gccattgaca cggtctgtgc attgaaggtg ttcgtttcga tgatgtccga cccggcttca
     3601 aaataagctt cgtgaatgcc ttgaatgatg ccgggctggg tcagcacaag aaggtcgttg
     3661 ttgcctttga gatcgctcgg ccaatccttg aagcgctcgc cacgataatc ggcctcctta
     3721 agcttgtggc gctggatcat ggttc
//
LOCUS       DATMGX010000094         2157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_152459, whole genome shotgun sequence.
ACCESSION   DATMGX010000094 DATMGX010000000
VERSION     DATMGX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152459"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1486
                     /gene="serS"
                     /locus_tag="VNR65_01545"
     CDS             194..1486
                     /gene="serS"
                     /locus_tag="VNR65_01545"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWJ17387.1"
                     /translation="MLDLGFVRDHLPLVEEKLRQRGMVPAAILKDFHAIDLERRAAIT
                     QAETLKAQRNKATEEIAKLKKDKLDATALINETKQLREKISEAEKVAEEADARLRNIL
                     TGIPNLPDDSVPVGKSEADNVEVRRWGTPPQFDFTPKPHWELGEELGVLDLERAAKLT
                     GARFAVYWDMGAKLERALMNFMLDLHTREHGYTEVLPPFLVNADSMYGTGQLPKFEAD
                     LFKVPHGDKNLYLIPTAEVPVTNLYRDETLDGARLPISLAAYTPCFRSEAGSYGKDVR
                     GIIRQHQFQKVELVKFTKPEQSWDEHEKLTRNAEAVLQKLGLHYRTVALCTADLGFSA
                     AKTYDIEVWLPGQGLFREISSCSNFTDYQARRANIRYRPEGGRKTELVHTLNGSGLAI
                     GRTWVAIVENYQQADGSVLIPEVLWPYIGSDRITAKKF"
     gene            1541..>2157
                     /locus_tag="VNR65_01550"
     CDS             1541..>2157
                     /locus_tag="VNR65_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HWJ17388.1"
                     /translation="MRKAVRFFPFVPAIMLLIPVIFGSLMSAQTSSVATPSQTTAQPG
                     TPTVAEAEEFIRQAETRLNDLTVKASRASWVQSNFITDDTEQMAADANEVLIGATTEL
                     AKQATRYDHLKLSDELTRKMLLLKLSAAVPAPAPNDPKELAEMARIGTSLEADYGKGK
                     YCPKSGKHAGECLDIGAIERIMAGSTDPEELKDLWVGWHTISPPMR"
BASE COUNT          475 a          658 c          627 g          397 t
ORIGIN      
        1 gaaagtcatt ccaagaagca aaaaacaaga agcctaaaac ctctgtgtct ctgtgcctct
       61 gtggtgaaaa acactttctt gcttttccgt gcgctccgtg ttctctgtgg tgcaaaatca
      121 ggtctctgtg gtggagtcgt ggtggagtct cctgcttggt gatcggcagc gtcttcgagt
      181 aacctgatct cttatgctcg atctaggctt tgtacgcgat catttaccgc ttgtcgaaga
      241 aaagctgcgg caacgtggca tggttcctgc cgccatcctc aaggattttc acgccatcga
      301 tctcgagcgc cgcgccgcta tcactcaagc tgagacgcta aaggcccagc gcaacaaggc
      361 gacggaagaa atcgccaagc tcaagaagga caagctggac gccactgcgc tgatcaacga
      421 gaccaagcag ctacgcgaga agatctccga agccgagaaa gtcgccgagg aggcggacgc
      481 aagactgcgc aacatcttaa ctggaattcc caacctgccg gacgacagcg tgcccgtcgg
      541 caagagcgaa gccgacaacg tcgaggtacg gcgctgggga acaccgccgc agttcgactt
      601 cacgcccaaa ccgcactggg aactaggcga ggagctcggc gtgctcgacc tggagcgcgc
      661 ggccaagctc acgggcgcgc gctttgccgt gtactgggac atgggcgcga agctggagcg
      721 ggcgctgatg aacttcatgc tcgacctgca cacgcgcgag catggctaca ccgaagtgct
      781 gccgccgttc ctggtcaatg ccgactcgat gtacggtact gggcagttgc cgaagttcga
      841 agctgacttg ttcaaggtgc cgcatggcga caaaaacctc tacctgattc ccaccgccga
      901 agttccggtg accaatctct accgcgacga aacgctcgac ggcgcgcgcc tgccgatctc
      961 gctcgcggcc tacacgccgt gtttccgcag cgaggccggt tcgtacggca aggatgtgcg
     1021 cggcatcatc cgccagcacc agtttcagaa ggtcgagcta gtgaagttca ccaagccgga
     1081 acaatcgtgg gacgagcacg aaaaactcac gcgcaacgcg gaagctgtgc tgcagaagct
     1141 cggcctgcac taccgcaccg tggcgctctg caccgcagac ttgggtttct cggcggcgaa
     1201 gacctatgac atcgaggtgt ggctaccggg acaaggtctc ttccgcgaga tttcttcgtg
     1261 ctcgaacttt acggactacc aggcgcgacg tgcaaacatc cgttatcgtc ctgaaggcgg
     1321 caggaaaacg gagttggtgc acacgctcaa tggcagtgga ctggcgattg ggcggacgtg
     1381 ggttgcgatc gtcgagaact atcagcaagc cgacggcagc gtgctcattc ccgaagtact
     1441 gtggccgtac atcggcagcg atcgcatcac ggcgaagaag ttctagaata gcgaaccgcg
     1501 gccgttcgcc atttacaatg attccggagg tcgtttgcac atgcgcaaag ccgtccgctt
     1561 tttccctttc gttccagcga taatgcttct gatccctgtt attttcggtt cccttatgag
     1621 tgcacagaca agttccgttg ccaccccgtc gcagaccaca gcccaaccag gtactcccac
     1681 ggtcgccgag gcggaggagt tcattcgcca ggccgagacg cggctcaacg atctcacggt
     1741 gaaggccagc cgcgcttcgt gggtgcagtc gaacttcatc acggacgaca ccgagcagat
     1801 ggcagccgat gccaacgagg tgctgatcgg cgccaccacc gagctggcca agcaggccac
     1861 gcgctacgac cacctcaagc tctccgacga actgacgcgc aagatgctgc tgttgaagct
     1921 ttccgccgcc gtacccgcgc ccgcgccgaa cgatcccaaa gaactcgccg agatggcgcg
     1981 catcggaact tcgctcgagg ccgactacgg taagggcaag tactgcccca agtcaggcaa
     2041 gcacgccgga gaatgtctgg acatcggggc cattgagcgc atcatggccg gcagcaccga
     2101 tcccgaagaa ctgaaggacc tgtgggttgg ctggcacacc atctccccgc ccatgcg
//
LOCUS       DATMGX010000095         3334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_251692, whole genome shotgun sequence.
ACCESSION   DATMGX010000095 DATMGX010000000
VERSION     DATMGX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251692"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..670
                     /locus_tag="VNR65_01555"
     CDS             449..670
                     /locus_tag="VNR65_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17389.1"
                     /translation="MSKLNIATVESLEIDVQTEAGSYDVAPSAVTGPSAVSSNASPTA
                     AACYDYDCDYGYYYYHGRNSRARNQAPLS"
     gene            725..3073
                     /locus_tag="VNR65_01560"
     CDS             725..3073
                     /locus_tag="VNR65_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HWJ17390.1"
                     /translation="MEPDLCFVAEDAPAGTMTAPKTIVKSALEPSEDRAWALATEGYD
                     PLRESSIESRFAVSNGFLGVRAGRAVTRGARLVIPPRTYVAGLFDTPEAEHAIPVLVP
                     APSWLNVHISVPGKPLADHPFDVPSHQMTLDMRRGLVLTQWHHCHANEVSMHLRTLRL
                     VSLSERAAGLQVIHLEIDKGEAEITLEASFEGLDLGLVCERLSSGLGVWRTQYSGKRL
                     GMATTLSLHLDGNELPAEVNGPLTCCWRWKCRPGQTVYFERMVALVRSDSEAVDPGPL
                     AREKVRAARKLGWRGMIAEHEAAWARRWECSDAVIEGDPAAQQDLRFAIYHLNSAANP
                     NDERVSIGARALTGEDYHGHVFWDTEIYLLPFYTWTWPEAARALLMYRFHTMGGARAK
                     AARMGRRGALYAWESADTGEEATPAEVTGPDRVVIPVLCGTQEQHISADVAYAVWNYW
                     QTTGDDDFLCDAGAEIILETGRFWASRAGLEGDGQSHIEGVIGPDEYHETIDESAYTN
                     VMARWNIRCALGVAALLRERWPDCWTRLSSSLSLGEAELEQWRVVADTIVDGLDAETG
                     LYEEFTGYFGLEKINLADYAGRTVPMDVVLGRHRTAKSQVVKQADVVALLALLPDEFP
                     GDLASKNFNFYEPRCGHGSSLSPSLHGLAAARLGESEMALGYLRKSAAIDLSDSHVGI
                     GGGIHIASLGGNWMLVVLGFAGLSLRSDGISLDPKLPAEWRSLAFAVQWRGRSLKIRI
                     RQDERDIEASLQDGEPMTLTVSGTPHLLRLDHPVRARFGAQR"
BASE COUNT          644 a          989 c         1035 g          666 t
ORIGIN      
        1 gcagtcaaat cggtgttttg gcagagaata aaacaataaa aaatgcaccg tttgaaaatt
       61 ttaaatctgc ggtgtgggga aaattctcat aacaggagct ttctgccatc aaggacgacc
      121 tggtttccca ggtccagaca aaatacggtt tgaataagga gccggctcta cgcgatattg
      181 atgcagtgct aatgtccaga cgatagactc ggatactact cacgcgctcg ccgaatcggt
      241 tgatctgtga cggactcttc ccttctcttg gtccactgag tactttccga ctctattaat
      301 ccagcagaaa ggctgtcact gttgatttca tgcagtagga ttcggtgtat ccgtcgccgc
      361 gacgcctttt gtgcgcaggc tgaataacaa tgcctgtggg ggtacggctc tcttagcctc
      421 cagccgcact ctgttcagga gaagagcaat gagtaagctc aatatcgcca ctgtcgaaag
      481 cctcgagatc gatgttcaga ccgaggccgg ctcgtatgac gtcgctccgt ccgctgtgac
      541 cggcccatcc gccgtgtcct ccaacgcctc cccaaccgcc gccgcctgtt acgattacga
      601 ctgcgactat ggctattatt attatcacgg ccgtaattcc cgggctcgca atcaggctcc
      661 gctctcctga tctcataatg ctgccggctg aaaacttcgc gcgcttgcca ctgagggact
      721 gaagatggaa ccggacttgt gcttcgtcgc cgaggacgcg cctgccggca ccatgaccgc
      781 gccgaagaca atcgtgaaat ccgctctgga gccgtccgag gatcgtgcgt gggctcttgc
      841 gacggagggc tacgatccgc ttcgcgaaag cagcatcgag tcgcgttttg ccgtcagcaa
      901 cggtttcctg ggcgtaaggg ccggccgcgc agtaacgcgc ggcgcgcggc ttgtgattcc
      961 accgcgcacc tacgttgccg gcctttttga cacgccggag gccgagcacg cgatcccggt
     1021 gcttgtcccg gccccgagct ggcttaacgt ccatatctcg gtgcccggca aacccttggc
     1081 ggaccatccc ttcgatgtgc cgtcgcacca gatgacgctc gatatgagac gaggccttgt
     1141 tctcacccag tggcaccact gccacgctaa cgaagtgagc atgcacctgc gcacgctgcg
     1201 cctggtttcg ctgagcgagc gcgcggccgg attgcaagtg atccacttgg agatcgacaa
     1261 gggagaggcc gagatcacgc tcgaggcgtc gttcgaaggg cttgatctcg ggctcgtctg
     1321 cgagcgtctc agcagcggcc tcggcgtatg gcgcacgcaa tattcaggca aacgtctcgg
     1381 catggcaacc accctgtctt tacatctcga tgggaacgag cttcccgcgg aagtaaatgg
     1441 cccgctgacc tgttgctggc gctggaaatg ccgccccgga cagaccgtat atttcgagcg
     1501 catggtcgcc ttggtgcgca gcgacagcga agcggtagac cccggccccc tggcacgcga
     1561 gaaggttcgc gcggctcgca aactcggctg gcgtgggatg atcgcggaac acgaggcggc
     1621 atgggctcgc cgatgggaat gcagcgatgc cgtaatcgag ggcgatcccg ccgcgcagca
     1681 ggacttgcgg ttcgcgatct accatctgaa cagcgcggcc aatcccaacg acgagcgcgt
     1741 ctcaatcggc gcccgggcgc tcaccggcga ggactaccac ggtcatgttt tctgggacac
     1801 cgagatctac ctgcttccat tttatacgtg gacatggccc gaagccgcac gcgcattgct
     1861 catgtatcgc tttcacacga tgggtggcgc gcgggcaaaa gcggcccgca tgggcaggcg
     1921 cggtgcgctt tatgcctggg agtcggccga taccggcgag gaggcaaccc cggctgaggt
     1981 taccggcccg gatcgcgtgg tcatccctgt tctgtgcggc acgcaggagc agcatatcag
     2041 cgcggacgtt gcctacgcgg tttggaacta ttggcaaaca acgggggatg acgattttct
     2101 ttgcgacgcc ggagcggaga tcattcttga gacaggccgc ttctgggcga gccgcgcggg
     2161 gctagagggg gatgggcaaa gtcatatcga aggcgtcatc ggacccgacg agtatcatga
     2221 gacgattgac gagagcgcct acaccaacgt catggcgcgc tggaatatca ggtgcgcgct
     2281 cggcgtagcc gcccttttgc gcgagcgctg gccggattgc tggacgcgcc tgtcctcgtc
     2341 gctcagcctc ggcgaggcgg agcttgagca gtggcgcgtc gtcgcggaca ccatcgtgga
     2401 tggccttgat gcggagaccg gactctacga ggaatttaca ggctatttcg gactggaaaa
     2461 gatcaatctc gcggattacg cgggccgcac cgtgccgatg gacgtggtgc ttggccggca
     2521 tcggacggca aaatcccagg tcgtcaaaca agccgatgtg gtcgctcttc tggcgttgct
     2581 accggatgaa ttcccaggtg acctggcgtc gaagaacttc aatttctacg agccccgatg
     2641 cggccacggc agctcgctga gcccttcctt gcacgggctc gcggcggcgc ggctcggcga
     2701 gagcgagatg gcgctcggct atctgcggaa gagcgccgcg atcgatcttt ccgacagcca
     2761 tgtggggatc ggcggcggca tccacattgc ttctctcggc gggaactgga tgctcgttgt
     2821 gctggggttc gcggggctgt cgctccgaag tgacgggatt tcgcttgatc caaagctgcc
     2881 ggcggagtgg cgcagcctgg ccttcgctgt gcaatggcgt ggccgcagtc tcaagatcag
     2941 gatccggcag gacgagcggg atatcgaagc gagcctgcaa gacggcgagc cgatgactct
     3001 caccgtgagc gggacgcccc acctcctccg gcttgaccat cctgtccggg cacgcttcgg
     3061 tgcgcaacgt tagagatttg acacacaggt tggactgtgt gggcgcatct cacttcggct
     3121 tcgggtcagg tgcgggattg tgtcggcctt atcggagaag tgctgcttcg ggatctagaa
     3181 gccgcctcgc ctcgcatagg atcgcgacaa agccgacacg accgcaaacg acccgcaaca
     3241 gaacggatcc aagcgttggc gaagccatgc atgtttgcct catggacgcc ttcctcccgc
     3301 gcttcgttat tacggccaag gaagagcgac acgg
//
LOCUS       DATMGX010000096         3260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_285538, whole genome shotgun sequence.
ACCESSION   DATMGX010000096 DATMGX010000000
VERSION     DATMGX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285538"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="VNR65_01565"
     CDS             complement(<1..1477)
                     /locus_tag="VNR65_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17391.1"
                     /translation="MNWSVEFFPFFSPELLAACAVFCVAAIALSAWRSRRGVVLRALS
                     LGCLLLALANPNLKNEDRKSLSNIAVVVADASSSMQLAGRNERARQIEAELKDKLAKI
                     PNLEVRWVHAAGTDQPGGEATNLFSALDTTMADIPPGRMAGAIFISDGQVHDAPQDQG
                     RLGFDAPLHALITGSPDEKDNRIQVVSAPRFGLVGSEQIAEVKAERSGKSSDGAPATF
                     LIRRASGAVETVIASYGKPVPIRLHFDRAGPNIVEVELKPEPGELTTINNKTVIQAQG
                     VRENLRVLLVSGEPHAGERTWRNLLRSDAAVDLVHFTILRPPEKHDGTPIDQLSLIAF
                     PTRELFSEKLSQFDLIIFDRYQRRAVLPMIYLDNVARYVEQGGAVLVAAGADYASPVS
                     LARTPLGTVLPARPTGRVVEQAFRPAITKEGEKHPVTAGLAGAPRKEGQEPSWGHWYR
                     VVDSQAERGEVVMSGAENKPLLILDRRGSGRVALLLSDHAWL"
     gene            complement(1505..>3260)
                     /locus_tag="VNR65_01570"
     CDS             complement(1505..>3260)
                     /locus_tag="VNR65_01570"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HWJ17392.1"
                     /translation="QDTTDLTNASPSVMILSDVGRISGNVAERLRGFVEKGGMLIRFA
                     GPRLEQGGDGLLPAPLREGGRTLGGAMSWASPQTPAAFEQGFPFEGLAIPKDVTVSRQ
                     VLADPAAMPRETQVWARLSDGTPLVTATKRGAGTLVFFHITANPDWSNLPISGLFVEM
                     MRKVLEIAPLQVSQSGNAPAGSVEASSINASQDASLKPWRVLDGFGKLSQPGERTQAI
                     PAARIEKARANAANPAGLYGPQNALRAINVVSETDMLSALKTPGNAHVMVFSDAKTVA
                     LAPWLFLAALGLFLADGVVCSLFLGTGSIQWRTRAAAIVAFGLFLGPVYGARADTKSP
                     NDATSFALQASLETHLAYVMTGNAEADRVSHAGLSGLSKILTARTAVEPGDPMAVDPA
                     RDELVFFPLIYWPVLPNAEPLPDAVLAKIDAYMKQGGLIIFDTKNDEASPGSLLSSGV
                     TTPLSQLLGKLDLPPLQHVPEDHVLTKSFYLLQSFPGRWDNGDPWVEARADTGDATKR
                     AVKSDGVSSIIVTSNDLAAAWAMDDNDRPMFATVPGGDDQREMSYRVGVNIVMYALTG
                     NYKSDQVHVPAILERLGH"
BASE COUNT          640 a         1018 c          992 g          610 t
ORIGIN      
        1 aaagccaggc gtgatcggac aagagcagcg caaccctccc gctcccacgg cggtccagga
       61 tcaggagcgg cttgttctca gcgccgctca ttacgacttc gccacgttcg gcttggctgt
      121 cgaccacgcg gtaccaatgg ccccagctcg gctcctgacc ctccttgcgc ggtgcgccgg
      181 cgagtcctgc ggtaaccggg tgtttttcgc cttcctttgt gatcgcgggc ctgaatgcct
      241 gctcgaccac acgccctgtt ggccgggccg gaagcacggt gcccagcggc gtgcgcgcga
      301 ggctgacggg cgaggcgtaa tccgccccag ccgcgacaag cacggcgccg ccctgctcga
      361 cgtaacgcgc aacattgtcg agatagatca tcggcagcac cgcgcggcgc tgatagcggt
      421 cgaagatgat gaggtcgaac tggctgagct tctcggagaa gagttcgcgc gtcgggaaag
      481 cgatgagcga aagctgatcg atgggcgtgc catcgtgctt ctcgggtgga cgaagaatcg
      541 tgaaatgaac cagatcgacc gcggcatcgg atcgcaggag gttgcgccag gtccgctcgc
      601 ctgcgtgggg ttcgccggaa accagcagca cgcgcaaatt ctcgcgcacg ccctgcgcct
      661 ggatcacagt cttgttgttg atggtggtca actcgcccgg ctcgggcttc aactcgacct
      721 cgacaatgtt cgggccagcc cggtcgaagt gaaggcggat tggaaccggc ttgccgtaag
      781 aggctatcac cgtttccacg gctcctgagg cgcgccgtat gagaaaagtg gcgggtgcgc
      841 cgtcgctgct tttcccgctg cgctctgcct tcacctctgc aatttgctcg gaaccgacga
      901 gcccgaagcg cggcgcagag acaacctgga tgcgattgtc tttctcatct ggcgatccgg
      961 ttatcagcgc gtgcaacggc gcgtcgaatc cgaggcggcc ctgatcctgc ggggcatcgt
     1021 gaacctggcc gtcgctgata aaaatcgcgc cggccatgcg gcccggcgga atgtcagcca
     1081 tggtagtgtc gagcgcgctg aagaggttgg tcgcttcccc gcccggctgg tccgtgccgg
     1141 ccgcgtggac ccagcgcact tccagattgg ggattttcgc gagcttgtcc ttcaactcgg
     1201 cctcgatctg gcgtgcgcgt tcattgcgtc cggccagctg catgctggag ctcgcatcgg
     1261 caacgacgac ggcaatattg gacagcgatt tccgatcttc gtttttcagg ttggggttgg
     1321 ccagcgcgag aagcaggcag ccgaggctca aggcccgcaa gacaacgccc ctgcggctcc
     1381 gccacgccga aagcgcgatc gccgccacgc aaaaaacagc gcaggccgca agcagctccg
     1441 gcgaaaagaa ggggaaaaac tcaacagacc aattcatgag gactattctg ctttttgtat
     1501 gcgattaatg cccgaggcgt tccaggatcg cgggcacatg gacttgatct gatttataat
     1561 tgccggtcag cgcatacatc acgatgttga cgccgacgcg ataggacatt tccctttggt
     1621 cgtcgccgcc aggaacggta gcaaacatcg gccggtcatt gtcgtccatc gcccaggcgg
     1681 cagccagatc gttcgaggtg acaatgatag agctgacgcc gtccgacttt acggcccgct
     1741 ttgtcgcatc tcccgtatcg gcgcgcgcct ccacccaagg atcgccattg tcccagcggc
     1801 cagggaatga ctgcagcaga taaaacgact tcgtcagcac gtgatcttcc ggcacatgct
     1861 gaagcggcgg cagatcgagt ttgcccagaa gctgcgataa gggggtggtc acgcccgacg
     1921 acaaaaggct gcctgggctc gcttcgtcgt tctttgtatc gaagatgata aggccgccct
     1981 gcttcatgta cgcgtcaatc ttggcgagaa cggcgtccgg caaaggctcg gcgtttggca
     2041 aaaccggcca atatatgagc gggaaaaaca cgagttcatc gcgtgcggga tcgaccgcca
     2101 tcggatcgcc cggctcaacg gcggttcgtg ccgtcaggat cttcgataac cccgacaggc
     2161 ctgcgtggct gacgcggtca gcttcggcgt ttcccgtcat cacataggcg agatgggttt
     2221 cgagagaggc ttgcaacgcg aaagacgtcg catcgttcgg cgacttcgta tccgcgcgcg
     2281 cgccataaac ggggccgagg aacagcccga acgccactat cgcagccgct cttgtccgcc
     2341 attggatcga gcctgtgcca aggaagagag agcagaccac gccgtcggcc aggaaaaggc
     2401 ccagtgccgc aaggaaaagc cacggcgcaa gcgcgaccgt cttggcgtcc gaaaacacca
     2461 tgacatgcgc attgcccggc gtcttgagcg cgctgagcat gtcggtctcg gagacgacat
     2521 tgatcgccct taacgcattt tgaggaccgt agagccccgc cggattggcc gcattcgccc
     2581 gcgccttctc aatcctcgca gcagggattg cctgcgtccg ctcgcccggc tgcgagagct
     2641 ttccaaaacc gtcaagaacc cgccatggct tcaaggacgc atcctggctg gcattaatgg
     2701 acgaggcttc tacggaaccg gccggggcat ttccgctttg cgagacttgc aacggcgcta
     2761 tctccaaaac tttccgcatc atctccacga acagccccga aatgggaagg ttagaccaat
     2821 ccgggttggc ggtaatatgg aaaaatacaa gcgttcccgc tccgcgtttc gttgccgtca
     2881 cgagaggcgt gccgtcggaa agccgcgccc aaacctgggt ttctctcggc atcgcggcgg
     2941 ggtccgccaa cacttgccgc gaaacggtta catctttcgg gatggcaagc ccttcaaagg
     3001 gaaagccctg ctcaaaggca gccggcgtct gcggcgatgc ccagctcata gcccctccaa
     3061 gcgtgcgtcc accttcgcgc aagggcgcgg gcagcaaacc atcgccgccc tgttccagcc
     3121 ttggtcccgc gaagcggatc aacatgcctc ccttttccac gaagccgcga agcctttcgg
     3181 ccacgttgcc cgaaatgcgc ccaacgtcgc tgaggatcat gaccgacggc gaggcattgg
     3241 tgaggtctgt tgtgtcctga
//
LOCUS       DATMGX010000097         5853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_251653, whole genome shotgun sequence.
ACCESSION   DATMGX010000097 DATMGX010000000
VERSION     DATMGX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251653"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1073
                     /locus_tag="VNR65_01575"
     CDS             324..1073
                     /locus_tag="VNR65_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ17393.1"
                     /translation="MANAPHILIVDDDRQIRASLARFLTASGFRISAAEDGAAMFAAI
                     EKGRFDLVVLDIMMPGEDGLTLCRRLRGASRIPVIFLTALSGEIDCVAGLELGADDYI
                     CKPVRPRELLARIRAVLRRVGGMTLQRERAPVVSYEFEGWRLDAVRRTLRNPKGVLVE
                     LTAGEFDLLLVFAEHPRNVLTRDQLLDLTRGREAAVLDRSIDVQVGRLRRKIEADPQL
                     PMLIKTVRSGGYVFCADVLVGSAEYVADAVS"
     gene            1060..2529
                     /locus_tag="VNR65_01580"
     CDS             1060..2529
                     /locus_tag="VNR65_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ17394.1"
                     /translation="MQFRKVRLGTRIAVTILAAILAVGALYAVLFLLMPARILTIYSA
                     HWLVGKAEEATSVIFQEGAKARDARAARFGADNHLQIRWRRTWDEPEPASEKGLRPFL
                     ERARASIEHDLEGKVRKVAVRGVLELRGNMLHVDLQPQPPDFLHRLPLGPLGHGEADV
                     PILGIFELAIQGLDESWIIIQPEGTQGYAERLRPFLILLIGAVSLVAFLSTVTARNTL
                     RPLERLTEGARNFGRTRKAVPIDPAGLHEFEVVARAMNEMQESIKRFIDERTHMLAAL
                     SHDLRTNLTGLLLDAEEVTGGDSKDRLIAGIEEMERVISATLAFAGDDLKGEPAQMID
                     LAALLISLCDSFSDRNCPASYSGPDHLLAMCQPVAIKRALTNLIDNAVKYGGCARVQL
                     ALFADGATISISDDGPGIPADKADLAFKPFGRLDYARNRESGGVGLGLTIARDIVQSH
                     GGEIRLGFPSRGKGFEVLISLPLPSSAPRLEAHQQQDGI"
     gene            2660..3040
                     /locus_tag="VNR65_01585"
     CDS             2660..3040
                     /locus_tag="VNR65_01585"
                     /inference="COORDINATES: protein motif:HMM:NF040078.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HWJ17395.1"
                     /translation="MNLEKVVFGFFILLAATLNFGFVYGDIGNPDQHNAWELFAAIIA
                     SLIAVILKFGDRTHIGAVHLASSLVALLQLFAAALVWFFATQKAPQLSAGSLASVVSL
                     SAGALFANVVSVIIMIIDTAIQRR"
     gene            3042..4751
                     /locus_tag="VNR65_01590"
     CDS             3042..4751
                     /locus_tag="VNR65_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWJ17396.1"
                     /translation="MQSVFFITIRRLRAPLILIILIFALSTMGLSLIPGVDAQGQAWR
                     MTLFEAFYFVTYTATTIGFGEIPNAFTNQQRLFVTLIIYLSVIGWAYLLGSLLGLSQD
                     KAFQQAIVSARFRRSVARLREPFYLVCGLGETGMTVVRALDMLGHRFTAIDRDERKIQ
                     ELEIEDLSMGAPLLVADARSPETLAAAGLLKPECKGVLTLSNDDAVNLAVAVSACLLR
                     PGLPVVGRADSPAVASSMSSLGTYRVINPFREFGEHMELAMRAPDSHRLLSWLTSTPG
                     SYLPPSLPPRIPAAPGHWIVCGYGRFGNEVVAAVQRGGFKATIIDPAGIRADGLRSIK
                     GLGTGTAVLSEAGVETASGIFAGTDDDTTNLAISIAARRLNPGIFVIVRQNLRANQML
                     FARFGADMTMVSSQIIANECIAVLRTPLLAEFLDLVRHKDDFWAYSLAESLRALMGEE
                     TPGFWSFSIDDTEAPGLFDLMHRIRRPVSIGDLCRSAKRRDDRIPCLALLAVRGTRTV
                     ELPNDDFEMEFGDRFLFAGQVAAEIDQSLMLQNANVAAYVLGARSEAEGWVWRKFEKA
                     LAR"
     gene            4866..>5853
                     /locus_tag="VNR65_01595"
     CDS             4866..>5853
                     /locus_tag="VNR65_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17397.1"
                     /translation="MKASAPIYFDITDIVEHAMHNSVVTGVQRSSIRIIECFIRNGCA
                     DQIYGFLKHPVSGEFKLCDLSFMKGAYDIGDFVARFGLPVGKEAWLAGKLLRYKDRPV
                     KRLLRKARLRAQWAAFPSLRKKIEDGRPAAAPCCLMPLGLKAGGIIVSLGASWGSDYV
                     GISELAKAHGCEVVSFVHDILSLSMPAKLVHGGKHKITKFVDWLKFIAENSSLLLCNS
                     AFTENDLRRYLGEQGMSARTAVVRFPHEFRVSSDQSGNIRSEVKALAAAKYALCVGRI
                     EARKNVIGLLKAWTRAREMPGAKAKLLIIAGKPGAMAGEVFEFLQRTENVSGT"
BASE COUNT         1161 a         1634 c         1745 g         1313 t
ORIGIN      
        1 cgtgccttga tcaacacctc tcatatcata agctcatctt gcttctgatc ttgtgccgct
       61 gaacagcttg agattgtatt gagtgcgccg cgtgagagtc ccgcgcaatc cgctttgaaa
      121 acgccgcgat cgtgacggcc atactggtcg ccacgatcac ttgcaggttg tgggtcctgc
      181 aagtctgtag cttaaacacc aacgcctcga atatgataag cgccgcaaga cgaatcgtaa
      241 cgtaatgtga caacagacgg gtgctagcca actgtcccgg ttgggcttat gacttgtcac
      301 accatttttc ggtggcatga ttcatggcga acgcaccaca tatcttgatt gtcgacgacg
      361 acagacagat cagagccagc cttgcccgct ttctaaccgc gagcgggttc cgcatttctg
      421 cggcggaaga tggcgcggcc atgttcgcgg cgatagagaa gggccgcttc gatctcgtcg
      481 tgttggacat catgatgcct ggcgaagacg gattgacgct gtgtcggcgc cttcgcgggg
      541 caagcagaat ccccgttatt ttcttgaccg ccctttccgg agaaatcgac tgtgtcgcgg
      601 ggctggagtt gggcgccgac gattacatct gcaagcctgt acgtcccagg gagttgctag
      661 cgcgaatccg agcggtgctg cgtcgcgtag ggggcatgac tcttcagcgg gaacgcgcgc
      721 cggtcgtctc ctacgaattt gaaggatggc gtctggacgc tgtgaggcgc acactgcgca
      781 acccgaaagg agtgctcgtc gagttgaccg ccggcgagtt cgatttgctt ctggtttttg
      841 cagagcaccc ccgcaacgtg ctaacgcgag atcaactcct cgatctgact cgtgggaggg
      901 aagcagcagt tttagaccga agcatcgatg tgcaggttgg caggcttcgt cgaaaaatcg
      961 aagccgatcc acagctcccg atgttgatca aaacagtgcg aagcggcggt tacgtattct
     1021 gcgcagacgt tctggtcgga tcagcggagt acgtcgccga tgcagtttcg taaggtccgg
     1081 ctcgggacgc gcatcgcggt cacgatactg gcggcgatcc ttgccgtcgg tgcgctctac
     1141 gctgtgctct tcctcctcat gcctgcacga atcctgacga tctacagcgc gcactggctt
     1201 gtggggaagg cggaggaggc cacatcagtc atatttcagg agggggcgaa agcaagggat
     1261 gcccgcgccg cccggtttgg agcggacaat catttacaga tccgctggcg gcgaacgtgg
     1321 gacgagcctg agcctgcatc ggagaaaggc ctaaggccct tcctcgaacg ggcacgggct
     1381 tccatcgaac acgatctgga aggaaaagtc aggaaagtgg ctgtgagagg cgtgcttgag
     1441 cttcgcggaa atatgcttca cgtcgatttg cagcctcagc cgccggattt tctacaccgg
     1501 ctgccattag gcccgctggg ccacggggag gcggacgtcc caattctcgg aatcttcgag
     1561 cttgcgattc aagggctcga tgagagttgg atcataatcc aaccggaagg gacgcaaggc
     1621 tacgcagagc ggctgcgacc cttcctgatc ctgttgatcg gtgcggtctc gttggttgca
     1681 tttctttcga ccgtcacggc aagaaacacg ctgcggccct tggaacggct taccgagggc
     1741 gcccggaact ttgggcggac gcgaaaggcc gttcccatcg atccggccgg cttgcacgag
     1801 ttcgaggtcg tcgcgcgggc catgaacgaa atgcaagaga gcatcaagcg ctttatcgat
     1861 gagcgtacgc atatgcttgc cgccctgtct cacgatctca gaaccaatct caccggcctg
     1921 ctcctcgacg cggaagaagt gaccggaggt gatagcaagg accgcctcat tgcgggcata
     1981 gaggagatgg aacgggtgat ctccgcgacg ttggcctttg caggcgacga tctcaagggc
     2041 gagccggcgc aaatgatcga tctcgcggct ttgcttatca gcctttgcga cagcttttcc
     2101 gaccgcaatt gccctgccag ctattccgga cccgatcacc ttctcgccat gtgccagccg
     2161 gtagcgatca aacgtgcact aaccaatctt atcgacaatg ccgtcaaata tggtggttgc
     2221 gcccgcgttc agcttgcgct ttttgccgac ggcgcgacga tttcaatctc tgacgatgga
     2281 cctggcattc ctgcagacaa agccgatctg gcattcaagc ctttcgggcg tctggactat
     2341 gcacgcaacc gggaaagcgg cggcgttggc ttgggtctta cgatagcgcg tgacattgtt
     2401 caatcgcatg gcggcgagat tcgcctgggc tttccttcgc ggggaaaagg gttcgaagtc
     2461 ctgatctcat tgcctttgcc ttcttccgcg ccccgtttag aggcacacca gcaacaggac
     2521 ggaatctagt gatccgccag ttcgtgctcg aaagcgtcgt ttccgaagtg ggcagagtgc
     2581 tctcccttga tctttcgcaa ggtccaggcg atgcaacagt gcttgagcta cctgatcggc
     2641 ggcggcgggc ggtggatcga tgaatctcga aaaggttgtc ttcggatttt ttatcctttt
     2701 ggcggcgacc ctgaatttcg ggttcgtcta tggtgacatc ggcaacccgg accagcacaa
     2761 tgcatgggag ctgttcgcgg cgatcatcgc cagtttgatc gcggtcatcc tgaagttcgg
     2821 cgatcgcacc catatcggag ccgtgcactt ggcttcgagc ctcgtcgcgc tcctccagct
     2881 tttcgcggcg gcgctcgtgt ggttttttgc gacacaaaaa gccccccagt taagcgcggg
     2941 gtcgctcgcc agcgtcgtgt ctctttccgc cggcgcgcta ttcgcgaatg tcgtatccgt
     3001 catcatcatg attatcgata cggccatcca gaggcgttga cgtgcaaagc gtctttttca
     3061 taacgatacg gcgccttcgg gctccgctca tcctgattat cttgatcttc gcattgtcga
     3121 caatggggct ctcgctcatt ccgggcgtcg acgctcaggg ccaggcctgg cggatgaccc
     3181 tgtttgaggc attctacttc gtgacctaca cggccaccac catcggattt ggagagatcc
     3241 cgaacgcttt caccaatcag cagcgtctct ttgtgacgtt gatcatttat ctgtccgtta
     3301 tcggctgggc ctaccttctc ggctccctcc tcggtctttc acaggacaaa gctttccagc
     3361 aggcgatcgt aagcgcgcga tttcgccgct cggtcgcgcg cttgcgagag cccttctatc
     3421 tcgtttgcgg cctcggcgag acaggcatga cggtcgtgcg cgcgctggac atgctcggcc
     3481 accggttcac cgccatcgac agagacgagc gcaaaattca ggagctggag atcgaagacc
     3541 tgtcaatggg cgcccctttg ctcgtggccg acgcgcgctc tcctgagacg ctcgcggccg
     3601 caggcctgct gaagcccgag tgcaaaggtg tgctgactct ctcgaacgat gacgcggtca
     3661 atcttgccgt cgcggtgtcg gcctgtttgc ttcggcccgg attgccggtc gtaggccgcg
     3721 cggattcgcc agctgtcgca tcctcgatgt cctcgctcgg cacctatcgt gtgatcaatc
     3781 cgttccgcga gttcggggag catatggaac ttgcgatgag ggcgccagac agccatcgcc
     3841 tgctttcctg gctgacgagc acgcccggct cctatttgcc gccaagcttg ccgccaagga
     3901 ttccggctgc gcccgggcat tggatcgtct gcggctacgg caggttcggc aacgaggtcg
     3961 tcgccgcggt gcagagaggt ggcttcaagg ccaccatcat cgatcctgcc gggatacggg
     4021 cggatgggct tcgatccatc aaggggcttg gcacgggcac ggctgtgctt agcgaagctg
     4081 gcgtggaaac ggcatccggg atattcgcgg gcaccgatga tgatacgacc aatcttgcga
     4141 tctcgattgc cgcccgccgt ctgaacccag gtattttcgt gattgtccgg cagaatttgc
     4201 gggccaatca gatgctgttc gcgaggtttg gggcggatat gaccatggtc tcaagccaga
     4261 tcatcgcgaa tgaatgcatt gccgttcttc gcaccccgct tctggcagag tttcttgatc
     4321 tggtccggca taaggatgat ttctgggcct attctctcgc ggaaagcctg cgcgccttga
     4381 tgggagagga gacgccggga ttttggagtt tctccatcga tgataccgag gcgcccggct
     4441 tgttcgacct tatgcatcgc attcgccggc cggtgagcat cggcgatttg tgccgaagtg
     4501 cgaaacgcag agacgatcgc attccttgcc tggccctcct cgccgtgcgc ggaacgagga
     4561 cggtcgaact gccgaacgat gattttgaga tggaatttgg tgatcgcttc ctgttcgcag
     4621 ggcaggttgc ggcggaaatc gatcagtcgc tgatgctgca aaatgccaat gtcgcggcct
     4681 acgttctggg agccaggagc gaggcggaag gatgggtgtg gaggaaattt gagaaagcct
     4741 tggcgcggta gcggcaagtt cattgctcgc ctctctaagg cgcgtcgtcc acagccgcag
     4801 gcttgacaaa gagcgtgccg gcgagccaat caccccgatt gccaatcaag ctctgggcga
     4861 aaaacatgaa agcatctgcg ccaatatact tcgatatcac cgatatcgtt gagcacgcca
     4921 tgcataattc ggtggtgacg ggcgttcaac gaagctcgat caggattatc gaatgtttta
     4981 taaggaacgg gtgcgccgat cagatttacg gctttttgaa gcatccggtc agtggcgagt
     5041 tcaagctttg cgacttatcg ttcatgaaag gggcctatga catcggtgat ttcgtggccc
     5101 gctttgggct tcctgttgga aaagaagcat ggcttgcagg caaactcctt aggtacaagg
     5161 atcgtccggt caagcggctc ttgcggaagg ccaggctccg cgctcaatgg gctgctttcc
     5221 caagccttcg aaaaaagata gaggatggca ggccggctgc agcgccctgt tgcctcatgc
     5281 cgctgggtct caaggcaggt ggcataattg tgtccctcgg tgccagttgg ggaagtgact
     5341 acgttggcat cagtgaactg gcgaaggcgc atggatgtga agtcgtatcg ttcgttcacg
     5401 atatcctttc actatcgatg cctgcaaaac tcgttcatgg cggaaaacac aaaattacga
     5461 aattcgtaga ttggctcaaa tttattgcag aaaattcctc cttgttgctc tgcaattctg
     5521 ccttcacgga aaacgacctg cgcagatacc tcggcgaaca gggcatgagc gcgcggaccg
     5581 cggtcgttcg attcccgcac gagttcagag tttcttccga tcaatccggc aacattcgca
     5641 gcgaagtgaa agcactagcc gcagcgaaat atgcactatg cgtcggccgg atcgaggctc
     5701 gcaagaatgt catcggcctc ctcaaagcat ggacccgtgc gcgcgagatg cctggcgcca
     5761 aagcgaagct cctgatcatc gcgggaaaac cgggcgcgat ggcaggggag gtcttcgaat
     5821 tcttgcaaag aaccgagaat gtttcgggaa cgg
//
LOCUS       DATMGX010000098         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_251578, whole genome shotgun sequence.
ACCESSION   DATMGX010000098 DATMGX010000000
VERSION     DATMGX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251578"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="VNR65_01600"
     CDS             <1..1328
                     /locus_tag="VNR65_01600"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWJ17398.1"
                     /translation="AGAVFWFLRWFLALFPAIALRFSVRAIAGAAALVCVTVYLALSG
                     ASIAAVRSYLMIAVVFLAILLNRPALSLRNVALSALLILVVLPDSLIDISFQMSYAAT
                     AALIAGYERFGRYLHFEAKSIRERLTWQPVYIIGAVLVTTGTAGLAVEPFSAYYFHNL
                     TSYAAIGNLLGGPPIDFVTMPAMIIALISMPFGLDEWPLKLMGFGIDAMMVVAKFVAS
                     FPGALITVPAFSFAALLVIIAGGLWLIIWRGPWRVAGLAAIGLGVAMTSVHEKPDIYI
                     DRDAKVVAVRDKDGKLQAPKSRRAYYTLAQWLKFDGDNRKPKEASVGAGWQCDAYSCL
                     AMVKGQLLSFTSKPDAIQEDCRRVAILVTPMDLRVPCPTPKYIFDRGALWEKGSAAIS
                     ISETRIAVSVAGDKRGVRPWAPERHRRAVIPVDPDDTLEKQDTGSESKE"
     gene            1494..1703
                     /locus_tag="VNR65_01605"
     CDS             1494..1703
                     /locus_tag="VNR65_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17399.1"
                     /translation="MNIASFGASLAVAAMCAGLTACAEDPKYPSLSKIDDLGTILTPE
                     ERQKAVQDLQRQEQSHTDAVKTASK"
BASE COUNT          407 a          567 c          590 g          439 t
ORIGIN      
        1 tggcgggcgc cgtcttctgg ttcctgcgct ggttcctggc gctttttcca gcaatcgcac
       61 tccgcttttc ggtgagggcg atcgcgggcg cggcggctct cgtttgcgtc accgtctacc
      121 tggccctgtc aggcgcgtcc atcgccgctg tacggtccta tctgatgatc gccgtggtat
      181 tcctggcgat ccttttgaac cggcctgctc tttcgctcag aaacgttgcg ctgtcggcgc
      241 tactcatcct cgtcgtgcta ccagacagtc tgatcgacat cagcttccag atgtcctatg
      301 cggcaaccgc tgccctcatc gcgggatatg agcgtttcgg gcgctatctg catttcgagg
      361 caaaaagcat ccgggagcgg ctcacttggc agccggttta catcattggc gccgtacttg
      421 tgacaacggg aacggccggc ctcgcggttg agccgttctc cgcatattat ttccacaatt
      481 tgacgagtta cgccgcgatc gggaacctgc tgggcggacc tccgatcgac ttcgtgacca
      541 tgcccgcgat gattattgcg ctcatttcga tgccgttcgg cctggatgaa tggccgttga
      601 agctgatggg cttcggcatc gacgcgatga tggtcgttgc gaaattcgtc gcctccttcc
      661 ccggcgcgct gataactgta cctgccttct cctttgctgc gctccttgtc atcattgcag
      721 gcggcctttg gctcatcatc tggcgagggc cgtggcgggt ggcaggcctc gccgctatcg
      781 gcctcggcgt cgcgatgaca tcggttcacg agaagcccga catctatatc gaccgcgacg
      841 cgaaagtggt cgcggtgcgg gataaggacg ggaaactcca ggctccgaaa tcgcgcaggg
      901 cctattacac ccttgcccaa tggctgaagt tcgacggcga taatcgcaag cccaaagagg
      961 cgtcggttgg cgcgggctgg caatgcgatg cctacagctg tctcgccatg gtcaagggac
     1021 agctcttgag cttcacctca aagcccgatg ccattcagga agactgccga cgtgtggcga
     1081 tcctcgtaac tccgatggat ttgcgggtgc cgtgcccaac cccaaaatac attttcgacc
     1141 gcggcgctct ttgggaaaag ggttcggctg caatctcgat ctcggagacc cgcatagccg
     1201 tttctgtggc cggcgacaaa cgcggcgtcc ggccctgggc gcctgagcgc cacaggaggg
     1261 ctgtcatccc ggtggatccc gatgacaccc tggagaaaca ggacaccggc agcgagtcaa
     1321 aggagtagca agtcgttaac agatcctgaa acaagctgtt acataattca aaaagacgtc
     1381 gacaaaaaat ccgtctagcg tctacagagg gcataagatt cgcgaggcgc ggggctgcca
     1441 tgcatcgagc ttgtcgtaag ttcgtgttgc gtgccgggtt tagtatagaa aaattgaaca
     1501 tcgcaagctt tggggcaagc ctcgcggtgg cagcgatgtg cgccggactg accgcctgcg
     1561 ccgaggatcc gaaatatcca agtctctcca agatcgacga cttggggacc attctgaccc
     1621 cggaagagcg tcagaaggcg gtgcaagacc tgcaaagaca ggaacagtcg cacaccgacg
     1681 ccgtgaagac cgcgagcaaa taacggtcag gcggcattct tcgacgtcat gaaagctgaa
     1741 atggaagtag aacaagcggt gcggccttag caattttatt gcaccgcttt agcgtttaat
     1801 acaacgacac aacgatctaa gagtaacgat ctaagagttt ttgcttcgcg cagccgaagc
     1861 aaatccgttg gattgaacgc aggcctcaga tctgggcagg ctttggaatt ttcagttgtc
     1921 ggcttgatgg tcgcggcggc tcaatcgtgc agcagcgttg aaaacgggcg ttcggaagga
     1981 gcttttgggc ctcgtgaact cgc
//
LOCUS       DATMGX010000099         4050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_251457, whole genome shotgun sequence.
ACCESSION   DATMGX010000099 DATMGX010000000
VERSION     DATMGX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251457"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VNR65_01610"
     CDS             <1..797
                     /locus_tag="VNR65_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HWJ17400.1"
                     /translation="LFIIVHQVYELWFKELIHDLDEAVACLKASTAAPGSSPHRPSCD
                     GDGGSEKTGSEGEHPAGSQEVYEAARMLRRCNEIMRLLVDQFTILETMLPTHFLTFRG
                     KLDAASGFQSDQFREIEFLCGQKDEKLLHHHAPDLEASAKLMRRLREPSLHDVFFEAL
                     RSIGGPPGTSGSNEGDGYEARVQAIRHLYQSERQHRDWIDVSERLVEFDELFMGWRLR
                     HIQLVERMIGTRTGTGGYTGSLYLRQTLDKKFFPELWEARTILQAE"
     gene            794..2176
                     /locus_tag="VNR65_01615"
     CDS             794..2176
                     /locus_tag="VNR65_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ17401.1"
                     /translation="MKTPTITIAGAGLVGPLLAISLRQRGFPVVLYERRADMRRVQVS
                     DGRSINLAVSARGIYALQQAGVWSELRDIAIPMRGRLMHLLTGELIFQPYGQGESEVI
                     NSVSRAALNIALLDAAEAAGVAIHFQQRCLGCARADDGIVVCLHDEQSGKETAIAAEV
                     VIGADGSSSAVRGEMSRSPEFRLSQERLDYGYKELTIPAGQGGSHAMETRALHIWPRG
                     SYMLIALPNVDGTYGCILFLPFESERGFAALDSKSRVVEFFRANFPDALALMPNLAEN
                     YFANPTGAMVTVRCFPWHQEGNILLLGDAAHAIVPFFGQGINCGFEDCTCFLELLDAA
                     RTSGAESIDWQKLFTDFESSRKPNTEAIADLALENFVEMRDRVADSHFLFKKKVDLAL
                     EARFAGLYVPKYAMVAFHRIPYAVAQQRGRIQDRLLDELCAGIHRLEDIDWSKAAMLV
                     NRDLTQLGKR"
     gene            complement(2345..3112)
                     /locus_tag="VNR65_01620"
     CDS             complement(2345..3112)
                     /locus_tag="VNR65_01620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ17402.1"
                     /translation="MAVTLSFANGVVLITGGSRGIGAACVRLFAEAGARVAFSYRQAS
                     SQAEALVRECGPHNCFAIASELDQSESAQALVAAAVEHYGRLDALVVNHGIAPDELPV
                     DQMTDARWRHTLAVNLDSAFALVRGAVAQMKAQQGPGHIVLISSASGQRGEARLCDYG
                     ASKGAIISLTKSLAAELASFGIYVNCVAPGWVNTDMAAPYLTDPALREGVLRTIPLRR
                     VGTPEEIAAPVLFLCTPHAGFMTGEIMNVNGGSVMVG"
     gene            complement(3315..>4050)
                     /locus_tag="VNR65_01625"
     CDS             complement(3315..>4050)
                     /locus_tag="VNR65_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWJ17403.1"
                     /translation="AANPLSAPLMLPRGVPAAGARIIRDGIGFCPSWIESFAVHYRVM
                     LELMRPDAPGIDTPDLPYHAVLSELDIDGRIRGRDPVNTSLYMWQRTMLINYILTVLV
                     DRVEMAHSVEGRVPFLDHHVSEYAASLPVHYKIRESTEKYVLREATRDVITPELYARQ
                     KFPFIAPPLRKAAKNAKRDPLQERCEDIIRSRSFSDQPFFDRAKTCAYLDSLTDADDV
                     RAGEASPIVLRLATLSLLQSRFSVSA"
BASE COUNT          844 a         1151 c         1224 g          831 t
ORIGIN      
        1 tgttgttcat cattgtgcac caggtctatg agctctggtt caaggagctg atacacgatc
       61 tggacgaggc ggtcgcctgc ctgaaagcct ctaccgctgc acccggctcg tcgccccatc
      121 ggccttcgtg cgacggggac ggcggctctg aaaagactgg ttcagaaggg gaacatccgg
      181 caggctcgca agaagtctat gaggcggcgc ggatgcttcg gcgctgcaat gagatcatgc
      241 ggctgctggt tgatcagttc accattctgg agacgatgct gcccacgcac ttcctcacct
      301 ttcgtggcaa actggatgcg gccagcggat tccagtcgga ccagtttcgc gaaatcgagt
      361 tcctttgcgg ccagaaagac gagaagctac tgcaccacca tgctcccgat ctggaggcct
      421 ctgcgaagct gatgcggcgt ctgcgcgaac cttcattgca cgacgtattc ttcgaggcgc
      481 tgcggagcat cggtgggccg cctggcacga gcggaagcaa cgaaggagat gggtacgagg
      541 cgcgtgttca ggccattcgc catctgtacc aatccgagcg ccagcatcgc gattggatcg
      601 acgtaagcga gcggctggtc gaattcgatg agctgttcat gggctggcgg ttacgccata
      661 tacaactggt ggagcggatg atcgggacgc gtacgggcac cggcggctac accggctccc
      721 tgtatctgcg gcagactctc gataagaagt tctttcctga gctatgggaa gcgcgcacga
      781 tcctgcaggc ggaatgaaga ctcccacaat cacaatcgcc ggcgctggac tggtggggcc
      841 gttgcttgcc atctcgctta gacagcgggg gttcccggtg gtcctctacg aacggcgcgc
      901 ggacatgcgc cgcgtccaag tcagcgacgg gcgctcgatc aatctggccg tttccgcgcg
      961 cggcatctat gcgctgcagc aagccggcgt gtggagcgag ttgcgcgaca ttgcaattcc
     1021 catgcgcggc cgcctgatgc atttgctcac cggcgaattg atctttcaac cttacgggca
     1081 aggggaatcc gaggttatca attccgtttc gcgcgcggcc ttgaatatcg cgctacttga
     1141 tgccgccgaa gccgcggggg tcgcaattca cttccagcaa cgctgcctcg gatgcgctcg
     1201 cgcagacgat ggcattgttg tgtgtttgca tgacgaacaa tcgggcaagg aaacagcgat
     1261 tgccgccgaa gtcgtaatcg gcgctgatgg ttcgtcttcg gctgttcgcg gggaaatgtc
     1321 caggtcgccg gagttcaggc tctcgcagga gaggctggat tacggctaca aagagttgac
     1381 aattcccgcc ggacaaggcg gcagccacgc aatggagacg cgcgcgcttc acatctggcc
     1441 gcgcggctcg tacatgctaa tcgccttgcc gaatgtggac gggacgtatg gctgcattct
     1501 gttcctgccg ttcgagagtg aacgtggatt tgcggccctc gattccaagt cgagggtggt
     1561 ggaatttttc cgtgccaact ttcccgacgc gctcgcctta atgccgaatc tggcggagaa
     1621 ctactttgcc aatcccacgg gcgccatggt taccgtccgc tgctttccgt ggcatcagga
     1681 aggaaacata ttgctcctcg gcgacgccgc gcacgcgatt gtcccgtttt tcgggcaggg
     1741 aattaactgc gggttcgagg attgcacttg cttcctggaa ttactggatg ccgcgcggac
     1801 gagcggcgca gagtcgatcg actggcagaa gctgttcact gacttcgaga gctcgcgcaa
     1861 gcccaatacc gaagccatcg ccgacctggc gttggagaac tttgtcgaaa tgcgggaccg
     1921 ggtggctgat tcgcatttcc ttttcaagaa gaaagtggac ctggcattgg aagccagatt
     1981 tgccggcctc tacgtgccca agtatgcgat ggtcgcgttc catcgcattc cgtatgcggt
     2041 cgcacaacaa cgcgggcgga ttcaggatcg tttgctggac gagctctgcg ccggcattca
     2101 ccgcctggaa gacatcgatt ggagcaaagc ggccatgctc gtgaatcgtg acttgacgca
     2161 gctggggaag cgataatgca ggccacgttt gaagccggtt gcggagtgct ttcagactgc
     2221 ggtcgggctc gagtagattc ggttcggtgc cgtaagcgat tcgctcatcg gcagaaactg
     2281 gaggcagaga gagaaggtcg cgtcccatag ctttcaatta acccggagtt cttcgccgaa
     2341 aaacttatcc caccatcacc gatccgccgt tcacgttcat gatttcgccc gtcatgaacc
     2401 ccgcatgggg agtgcataga aaaagcaccg gcgccgctat ttcttcgggc gtacctacgc
     2461 ggcgtaaagg aatggttcgc aaaacgcctt ctcttaaggc aggatcggta aggtagggcg
     2521 cggccatgtc ggtattcacc cagccgggtg caacgcagtt cacgtaaatg ccaaatgaag
     2581 ccagctcggc agcgaggctc ttggtcaggc tgatgatggc gcccttcgaa gccccgtagt
     2641 cgcagagacg ggcttcgccg cgctgaccgg aggcggagct gatgagaacg atgtggcctg
     2701 gaccttgctg cgccttcatc tgcgcgactg cgcctcgcac cagggcaaaa gcactatcca
     2761 ggttcacagc cagtgtgtgt cgccaccgcg catcggtcat ttgatcgacg ggcaactcat
     2821 ccggcgcaat gccatgattc accaccagag cgtcgagcct tccataatgc tctacggctg
     2881 cagcgacgag ggcctgcgcc gactcggatt gatccagttc agacgcaatc gcaaagcaat
     2941 tgtgcgggcc gcactcgcgc accagagctt cagcctggct actcgcctgg cgataactga
     3001 aggccacgcg cgcgcccgcc tctgcaaata accggacaca agccgcgcca attccgcgcg
     3061 aaccgccggt gatgagaacg actccattcg caaaggacaa tgtgacggcc acagttcttg
     3121 ttttcctccg aggaaggtaa tggaacacat tgacttaagc ccgaaaaatt caggacccta
     3181 acgcgtgatc tcgcaattca aatacgtttg cccttgaatc taccattgca catcattgcc
     3241 gagccatcac attggcaaag ttgatggcta gttgcgcaga aacgagactg ctcaaaagga
     3301 aatgggcggc ggggctaggc cgaaacgctg aaacggcttt gcagcagact gagtgtcgcc
     3361 agccgcaaga caatcggcga tgcctctccg gcgcgcacat catcggcatc tgtcaacgaa
     3421 tcgaggtagg cgcaagtttt ggcccgatcg aaaaagggtt gatcagaaaa gctgcgcgag
     3481 cggatgatgt cctcgcagcg ttcctgcaat ggatctctct ttgcgttctt cgccgccttg
     3541 cgaaggggtg gcgcgatgaa agggaacttc tgacgcgcgt agagttctgg tgtgatcacg
     3601 tcccgtgtgg cctcgcgcag tacgtacttt tctgtgcttt cgcgaatctt gtagtgaacg
     3661 gggaggctgg ccgcatattc gctgacatga tgatcgagga atggcacacg cccctcgacc
     3721 gaatgtgcca tctccacgcg atcgaccaac acggtcagaa tgtaattgat cagcatcgtt
     3781 ctttgccaca tgtagagcga tgtgttcacc ggatcgcgtc cgcggatgcg gccgtcgatg
     3841 tccagttccg aaagaacggc atgatacggc agatcaggcg tatcgatgcc gggtgcgtcg
     3901 ggccgcatga gctctaacat cacgcggtaa tgcacggcga agctttctat ccaggagggg
     3961 caaaagccga tcccgtcgcg aatgatgcgt gctccagccg caggcacgcc ccgcggcaac
     4021 atcaagggcg cggaaagcgg attggccgcg
//
LOCUS       DATMGX010000100         3601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_445516, whole genome shotgun sequence.
ACCESSION   DATMGX010000100 DATMGX010000000
VERSION     DATMGX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445516"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VNR65_01630"
     CDS             complement(<1..1449)
                     /locus_tag="VNR65_01630"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HWJ17404.1"
                     /translation="MSHLRRPKSDLGTIVLHWLIVLALCGAALTGLAIAALDNPDLRI
                     LPYVSFLLPSENVWYLHLGFGIALVASFVAYAVYVKRTDLTDRIRLSKSRLRGLMISG
                     RPRWAAVNVLLYWVLFIALVLETVVGTLLFFGWGGSLLTLHLHAVWLFLAFPFLHALG
                     HWLYGGEGQLLRIFRPQLRLPQRAPQLLEALIERVQRLEVENASPPPAVGEQRVTTPA
                     RLIKSARPTTAAVPLAIATTVGAAIAFVSPMVDTQSRQALRIVRINAAEAPVIDGDVS
                     DAAWRRALAQTIVTQHGANFDGGESKVEVRALHDGTFAYFAFTWTDPTRSLKHMPLVK
                     REDGWRLMRSASPGNEAQLHEDKFAVLLAAGGQHLIGKGIHFGRQPIPGKPEGATGRG
                     LHYIAGGYGDIWQWRASHGGLNGRVDNGHFGPPLPAAAAHSAGRYTGGFELDPGTLPY
                     QNNFELRSDRDTYPLVWPRSFPKSRDTLARLQS"
     gene            complement(1819..2142)
                     /locus_tag="VNR65_01635"
     CDS             complement(1819..2142)
                     /locus_tag="VNR65_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17405.1"
                     /translation="MDMKAGAIGAAVFGALLFGMTHAESAPPSYKAPDETVTLIPGPN
                     AKVAEDYCGACHSHEYILTQPRGAGFGRDFWKAEVTKMIKVFGAPINEADANAIVEYL
                     ASTYK"
     gene            complement(2148..3320)
                     /locus_tag="VNR65_01640"
     CDS             complement(2148..3320)
                     /locus_tag="VNR65_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWJ17406.1"
                     /translation="MNATLLYGAVRALPAEAAASVTLPFDNGARPLVRFPQKRPLIGL
                     TTRPPQLETPMAVYNESVLTPNDAFFVRYHLSRAPALIDPETFRLEVKGKVNTPLTLT
                     LEDLKRFEPIEVVAVNQCSGNGRGFSNPRVAGGQLGNGAMGNARWKGVPLKAILDKAG
                     VQEGAKQVFFNGADMPAAETIPDYIKALDIDHARDGEVLVAYAMNNEDLPILNGYPLR
                     LVVPGYYGTYWVKHLNEITVIDNVYDGFWMKTAYKIPNNSCACVAPGTKPASATPINR
                     MNVRSFVTSLADGAKVSAGDILTISGIAFDGGYGVTEVVVSTDGGKTWRGADLAADLG
                     KYSFRQWSTRLVLNKGNHTLAVRAINRIGQSQPVEALWNPAGYMRNSIEKVRVIAS"
     gene            3418..>3601
                     /locus_tag="VNR65_01645"
     CDS             3418..>3601
                     /locus_tag="VNR65_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17407.1"
                     /translation="MRLQPSFDCAEQETTYVILPFKRLPGPIGAVILPRRFVAFQILF
                     YTGEQRFFLARAQVTAI"
BASE COUNT          800 a         1008 c         1041 g          752 t
ORIGIN      
        1 agactggagc cgggcgagcg tatcgcggct cttgggaaaa gagcggggcc acaccaaggg
       61 ataggtgtcg cgatcggagc gaagttcgaa gttgttttga tacggaagag taccgggatc
      121 cagctcgaag ccgcccgtat accgccctgc ggaatgtgcc gcggccgccg gaagcggcgg
      181 tccgaaatgc ccattgtcta cccggccgtt caggccgccg tgggacgcgc gccattgcca
      241 gatatcgccg tagccgcctg cgatgtaatg caggcctcgg cctgtcgcgc cttcaggttt
      301 gccaggaatg ggctgccgtc cgaagtggat gccttttccg atcaaatgct ggccgcccgc
      361 tgccagcaaa actgcgaatt tatcctcgtg aagctgggcc tcattgcccg gcgacgcgct
      421 ccgcatgagc cgccagccgt cttcccgttt cacgagcggc atgtgcttga gcgagcgcgt
      481 agggtctgtc caggtgaacg cgaaataagc gaatgtgccg tcgtgcagcg cgcgaacttc
      541 aacctttgac tcgcctccgt cgaaattcgc cccatgctgc gtgacgatcg tttgagccaa
      601 agcacgccgc caggccgcgt ccgagacatc accgtctata accggggcct ccgccgcgtt
      661 tattctcacg atcctaaggg cttggcgcga ttgggtatcg accatgggac ttacaaacgc
      721 gatggccgct ccaaccgtcg tggcaatggc aagcggaacc gcggccgtcg taggcctagc
      781 ggacttgatc agcctggccg gcgtcgtaac gcgctgttcg ccaacggcag gcggcgggga
      841 tgcattctcg acttccagtc gctgcacgcg ttcaatcagc gcctcaagaa gctgcggcgc
      901 gcgttgagga agccgcaatt gcggcctgaa gatccgaagc aactgcccct cgcccccata
      961 aagccaatgc ccaagggcat ggaggaaagg gaaggcaaga aacagccaga cggcgtgcaa
     1021 atggagcgta aggagagatc cgccccagcc gaaaaagaga agagttccaa cgaccgtctc
     1081 gaggacgaga gcgatgaaca gaacccagta gaggagaacg ttgacggccg cccagcgagg
     1141 ccggccgcta atcataaggc ccctcagccg cgatttgctc aggcgaatgc ggtcagtgag
     1201 atcggttcgt ttgacgtaga cggcataagc cacgaacgaa gcaacaaggg cgatcccgaa
     1261 cccgaggtgc agataccaga cgttctcact cggaagaagg aagctgacat aaggcaggat
     1321 tcgcaagtcg ggattatcga gagcagcgat cgcaagcccc gtaagcgcag cgccacacaa
     1381 cgccaagacg atcagccagt gaagaacgat ggtgcccaga tcacttttgg gccgtctcag
     1441 atgtgacata ccttatacgc tcgcagaact gatactctaa ctgcgggcag cgccaatact
     1501 ggagatactg acgtcaacac agcgaggatt tccgagctat aggttatcta ttttgatgac
     1561 tatacgtagg cgggaatcag ataaattgtg tcgaaatatt gaatcaatcc taaagtcaaa
     1621 atccggaatt agttcaatgt attgaaccta aaaaacttca ttaggatgcc ggatgcaaca
     1681 aagtaatagc gctacagttt aatgcagaaa cttcatcacc ttcgcatgcc tgcggataag
     1741 actggcggcg cagtctcccg cgcaaggaca cgtaagcacc ctcggccgtt gcctcaagaa
     1801 agaggagctc acgatcgttc atttgtaagt gcttgcaagg tattcgacga ttgcgtttgc
     1861 atcggcttcg ttgatcggtg cgccgaagac tttgatcatc ttggtaactt cagccttcca
     1921 gaagtcgcgg ccaaagccgg ctcctcgcgg ctgtgtgagg atgtattcgt gggagtgaca
     1981 agcgccgcaa tagtcctcgg caaccttggc attgggtcct gggatcaacg ttacggtttc
     2041 gtcaggcgcc ttataggaag gcggagcgct ctctgcgtgt gtcatcccaa aaagaagcgc
     2101 gccaaagacg gcggcaccta tagctccagc cttcatgtcc atggctccta gcttgctatg
     2161 actctgactt tttcgatgga gttccgcatg tatcctgcgg gattccacaa agcctcgaca
     2221 ggctgggact ggccgatgcg gttgatagct ctcacggcaa gcgtgtggtt gcccttattg
     2281 aggaccaggc gtgtcgacca ttgcctgaaa gaatacttcc cgaggtcggc cgcgaggtct
     2341 gcgccgcgcc aggtctttcc gccgtcggtg gagacaacaa cctccgtaac cccgtatccg
     2401 ccatcgaaag caatgccgct gatcgtgaga atgtccccgg cagaaacctt tgcgccatcg
     2461 gcaaggctcg tgacgaatga gcggacattc attcgattga tcggcgtggc ggatgcgggc
     2521 ttcgtgccgg gcgcgacaca agcacatgaa ttgttcggga tcttgtaggc tgtcttcatc
     2581 cagaagccat cgtaaacgtt atcgatgacg gttatctcat tgaggtgctt gacccaatag
     2641 gttccatagt agccaggaac gacaaggcga aggggatagc cgttgagaat cgggagatcc
     2701 tcgttgttca tggcgtaggc aaccaagact tcgccgtcgc gggcgtggtc gatatcgagc
     2761 gccttgatgt aatctggtat cgtttcggcg gccggcatat ctgcgccatt gaaaaagacc
     2821 tgcttggcgc cttcctgcac tcctgctttg tcaaggatgg ccttaagggg aacgcctttc
     2881 cagcgggcat ttcccatcgc gccattgcca agctgacctc ccgcaacacg gggattggaa
     2941 aagccgcggc cgttccctga gcattggttg accgccacca cctcgattgg ctcgaaccgt
     3001 ttgaggtcct ccagcgtaag tgtcaacggc gtgttgacct tcccttttac ctccaaacgg
     3061 aaagtctcag ggtcaatcag tgcgggagcc cggcttaggt gatagcggac gaagaacgca
     3121 tcgttcggcg tcagcaccga ttcgttgtaa acggccatgg gcgtttccag ctggggcggc
     3181 cgagtcgtaa gcccgatgag cggccgcttc tggggaaacc gcacgagagg gcgtgcgccg
     3241 ttgtcgaatg gcaaggtgac actggcggcg gcttccgccg gcaaagctcg caccgctcca
     3301 taaagcagcg tcgcgttcaa tccaactctc agcagtttcc gccgagatat aaactcagag
     3361 cttccagtca tgtaactaca taccccggtt ctatgatgtc tgccggttag taattctttg
     3421 cgtttacaac cgtcgtttga ttgcgcagag caggaaacaa cctatgtaat actgccgttc
     3481 aagcggcttc ctggtccgat aggagctgtt atcttaccgc gccgttttgt tgctttccag
     3541 atattgtttt atacgggcga gcagcgcttc tttctcgctc gggctcaggt cacggctatt
     3601 c
//
LOCUS       DATMGX010000101         2692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_445285, whole genome shotgun sequence.
ACCESSION   DATMGX010000101 DATMGX010000000
VERSION     DATMGX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445285"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..470
                     /gene="rpsL"
                     /locus_tag="VNR65_01650"
     CDS             96..470
                     /gene="rpsL"
                     /locus_tag="VNR65_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWJ17408.1"
                     /translation="MPTFNQLVRKGRTAPKYKTASPALQSCPQKRGVCTRVYTQTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGVGHNLQEHSIVLIRGGRVKDLPGVRYHVVRGTL
                     DSVGVANRKQSRSKYGAKRPKA"
     gene            478..948
                     /gene="rpsG"
                     /locus_tag="VNR65_01655"
     CDS             478..948
                     /gene="rpsG"
                     /locus_tag="VNR65_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWJ17409.1"
                     /translation="MPRKGHIAKREPLADPVYQSTLVNKFVNSMMWDGKKSTAQNIFY
                     RAMKKLEDKGGDEAIKLFKKAVENAKPVLEVKTRRVGGANYQVPVEVNADRRTSLAIR
                     WILTYSRGRGEKGMIDKLANELLDAANGRGAAIKKKDDVHRMAEANKAFAHYRW"
     gene            1175..>2692
                     /gene="fusA"
                     /locus_tag="VNR65_01660"
     CDS             1175..>2692
                     /gene="fusA"
                     /locus_tag="VNR65_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWJ17410.1"
                     /translation="MPRQVPLDRCRNIGIMAHIDAGKTTTTERILFYTGRTHRIGEVH
                     EGTATMDWMAQEQERGITITSAATTCVWKDIRINIIDTPGHVDFTAEVERSLRVLDGA
                     CAVFDAVHGVEPQSETVWRQADKYGVPRICFINKMDKMGADFEHAIDTIRKRLNARPV
                     AIQLPIGQEDKFKGVIDLMAMKAIIWNDESLGAEYEVEEIPAELKKKAAAYHQILIET
                     VVENDDQDELMHKYIEGETIGEAELKAALRRATLKMKVFPVICGTAFKNKGVQPMLDA
                     VVDYLPSPLDVLPVKGIDPNTGKEVERPADDNAPFSALAFKIMTDPFVGQLTFIRVYS
                     GHLKQGDSVLISGRSRTERIGRLLKMHANKREDITEIYAGDICACVGLRHITTGDTIC
                     DEDQPVVLESIEFPAPVIAVAVEPKTKSDQEKMGVALGKLAQEDPTFKVNTDPDSGQT
                     IIAGMGELHLEILVDRMMREFNVQANVGKPQVAYRETIRATVESEGKFVRQSGGRG"
BASE COUNT          630 a          762 c          830 g          470 t
ORIGIN      
        1 aaaggtgcgg ccatggttgc cgccaagagt tttgctgtgg cgttaagccg tgagccagtt
       61 tagagctggc gtgaagaacg aaggagctgc aaagagtgcc tacatttaat cagctggtgc
      121 gcaagggacg gacggcgccg aagtacaaga cggccagccc cgccctgcag tcctgcccgc
      181 aaaagcgcgg tgtctgcacc cgcgtgtaca cgcagacgcc gaagaaaccg aattccgcgt
      241 tgcgcaaagt cgcgcgcgtc aggctgacca atggcatcga ggtaacgacc tatattcccg
      301 gtgtcggcca caacctgcag gagcactcga ttgtgctgat tcgcgggggc cgcgtgaaag
      361 atctgccggg cgttcgctat cacgtggtgc gcggcacgct ggattccgta ggcgtcgcca
      421 accgcaagca gagccgctcc aagtatggag ccaagaggcc gaaggcttag aggatttatg
      481 ccgagaaaag gacatatcgc gaagcgtgaa cccctggccg acccggtcta tcagtccacg
      541 ctggtcaaca agttcgttaa ctccatgatg tgggacggca agaaaagcac cgcccagaac
      601 atcttctacc gggccatgaa gaagcttgaa gacaaaggcg gggacgaggc catcaagctg
      661 ttcaagaagg ccgtggagaa cgccaagccg gtgctggaag tgaagacccg tcgcgtgggc
      721 ggcgccaact atcaggtccc ggttgaggtc aatgctgacc gccgtacgtc gctggccatc
      781 cgctggatcc tgacctacag ccgcggccgt ggcgagaagg gcatgatcga caagctggcg
      841 aatgagttgc tcgacgcggc caatggccgt ggagccgcca tcaagaagaa agatgacgtg
      901 caccgcatgg ctgaggccaa caaggccttt gctcactacc gctggtagag cagccagggc
      961 ttcggtgtat tcgtgagatg tgcttggttt gcaagggtga atgaagctgt aaacggcagc
     1021 cccccgcagg ggcggaataa cttagcccag cacgcagccg aaaccggagt gctgggtaag
     1081 acggaaaatg agcccgagtc ccatagggac gacacaggtt ctaccgacga attaccgcga
     1141 ctgcaaagtc gcatagcaag aaacggaaaa gattgtgcct cgacaggttc cattagatcg
     1201 ttgcaggaat atcggcatca tggcccacat cgatgccggt aagaccacca ccacggagcg
     1261 catcctgttc tacacgggac gcacgcaccg cattggtgag gtccatgaag gcaccgcgac
     1321 gatggactgg atggcgcagg agcaggaacg cggcattacg atcacgtcgg cggcgaccac
     1381 ctgcgtttgg aaagatattc ggatcaacat tatcgatacg cccggccacg tggacttcac
     1441 cgccgaggtc gagcgctcgt tgcgtgtact tgatggtgct tgcgccgtgt tcgatgccgt
     1501 acacggagta gagccgcaat ccgagacggt ttggcgtcag gccgacaagt acggcgtgcc
     1561 gcgcatttgc ttcattaaca agatggacaa gatgggcgct gacttcgaac acgcgatcga
     1621 tacgatccgc aagcgcctca atgcgcgccc tgtcgccatt cagttgccca tcggccagga
     1681 agacaagttc aagggcgtca tcgatctcat ggccatgaag gcgatcatct ggaatgacga
     1741 gagcctgggc gccgagtatg aggtcgagga aattccggca gagctgaaga agaaagccgc
     1801 cgcctaccat cagatcctga tcgagaccgt cgtcgaaaac gacgatcagg atgagttgat
     1861 gcacaaatac atcgaaggcg aaaccattgg cgaggccgaa ctgaaggccg cactgcgccg
     1921 tgcgacgctc aagatgaaag tcttcccggt gatctgtggg accgccttca agaacaaggg
     1981 cgtgcagccc atgctcgacg ccgtggtgga ctatctgcct tcgccgctcg atgttctgcc
     2041 cgtcaagggc attgacccca acacgggcaa ggaagttgag cggcccgcgg acgacaatgc
     2101 gccgttttcc gccctggctt tcaagatcat gaccgacccg tttgtcggtc agcttacgtt
     2161 catccgggtc tactccggac atctgaagca gggcgacagc gtgctaatca gcggtcgttc
     2221 gcgcacggag cgtattggcc gcctgctgaa gatgcacgcc aacaagcgtg aggacatcac
     2281 tgaaatctac gccggcgata tctgcgcatg cgtcggtctg agacacatca ccacgggcga
     2341 taccatttgc gacgaggacc agccggtcgt gctcgaatcc atcgagtttc cggcccctgt
     2401 gattgcggtc gccgtcgagc cgaagaccaa gagtgaccag gaaaagatgg gcgtcgcgct
     2461 gggcaaactg gcgcaggaag atcctacttt caaagtcaac accgatcccg acagcggcca
     2521 gaccatcatt gctggcatgg gcgagcttca cctggaaatc ctcgtcgatc gcatgatgcg
     2581 cgagttcaac gtgcaggcca atgtcggcaa gccgcaggtc gcgtatcgcg agaccatccg
     2641 ggcaaccgtg gagtccgaag gcaagttcgt gcgccagtcg ggcggtcgcg gc
//
LOCUS       DATMGX010000102         3256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_151557, whole genome shotgun sequence.
ACCESSION   DATMGX010000102 DATMGX010000000
VERSION     DATMGX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151557"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /gene="hisB"
                     /locus_tag="VNR65_01665"
     CDS             <1..258
                     /gene="hisB"
                     /locus_tag="VNR65_01665"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012687.4"
                     /note="catalyzes the dehydration of
                     D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate to
                     3-(imidazol-4-yl)-2-oxopropyl phosphate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HWJ17411.1"
                     /translation="PYLIWRVRFPAEKIGAMDTELFREFFPALAQNAGITLHIEALYG
                     ENAHHIAESCFKGLARALRSAITIDEEMAGEIPSTKGQLGD"
     gene            280..765
                     /locus_tag="VNR65_01670"
     CDS             280..765
                     /locus_tag="VNR65_01670"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HWJ17412.1"
                     /translation="MIIYTVHEPQRPGQTIEARADSIVFVKEGFTIWGFLFGPLWLLY
                     NRLWLAFILTLVVMAGLAAALIELELRNQAPAIVDLLASLIIGFEGNNILRWGLQRKG
                     YALLGSVVGRSRLECERRFFDAWLPHAAGKGAAAGTPVMGSKSGDWQTAHTIGWPEAT
                     A"
     gene            769..1419
                     /gene="hisH"
                     /locus_tag="VNR65_01675"
     CDS             769..1419
                     /gene="hisH"
                     /locus_tag="VNR65_01675"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWJ17413.1"
                     /translation="MFVAIVDYQSGNLHSAQKAFERATRESGLSYEIIVTGDPDAVLR
                     ADRVVLPGVGAFGDCYRGLSETPGMIDALKEAVLKRGRPFFGICVGMQMLADRGLEHG
                     THQGLGWIGGEVDLIKPSDKSLKIPHMGWNTLSFARSHALTEGIPDGPDGWHAYFVHS
                     YQFLPEDGDDVLARSDYGGPITALVARGNIAGSQFHPEKSQKLGLKLISNFLQWRP"
     gene            1723..2457
                     /gene="hisA"
                     /locus_tag="VNR65_01680"
     CDS             1723..2457
                     /gene="hisA"
                     /locus_tag="VNR65_01680"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HWJ17414.1"
                     /translation="MILYPAIDLKNGQCVRLRQGLMDQATVFNESPADQAYQFAQEGF
                     SALHVVDLDGAFEGRSVNAKAVSSILASVSIPVQLGGGIRTLEAVSLWLSRGVSRVIL
                     GTVAVRNPSLVKAACARFPGKIVVGIDAKDGRVAVEGWAETSELYATDLARRLQDVGV
                     AAIIYTDIARDGMLTGLNLDATADLARSVSIPVIASGGLAGIEDVKRLISPAYAIIAG
                     AIAGRALYDGRLDAKLALQLIGKGHE"
     gene            2651..>3256
                     /locus_tag="VNR65_01685"
     CDS             2651..>3256
                     /locus_tag="VNR65_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17415.1"
                     /translation="MVKPPLLSGNRVNPAAAILLSLILSAGLYAPRAHGEGDDAAPTA
                     PTSDTMKPAEKAPEESKKTPEESKKQAEESPSGKPESTAEEPKEAKPQAPVAGSKPAL
                     AKPAEASKPAKPAEPKETRAEKPKDLTPKPDEAGDLAVRASTVDAVYQISWLGTHIGD
                     FRIKSSITTRQYSLQANAELSVFFGTFSWQGVTTSSGLMTAN"
BASE COUNT          676 a          964 c          941 g          675 t
ORIGIN      
        1 ccttacctca tctggcgcgt tcgcttcccc gccgagaaaa tcggcgcaat ggatacggag
       61 cttttccgcg agttcttccc ggcactcgcc cagaacgccg ggatcaccct tcacatcgag
      121 gcgctctacg gcgaaaatgc tcatcatatc gcggaaagct gcttcaaggg cttggcgcgg
      181 gccttgcgga gtgccatcac tatcgatgaa gagatggcag gcgaaattcc atccacgaaa
      241 ggacagctcg gggactgatg cccctgctgg aaaacgccca tgattattta taccgtgcat
      301 gagccgcagc gtccgggcca aacgatcgaa gcgcgagccg acagcattgt cttcgtcaag
      361 gaaggcttta cgatctgggg ttttctcttc ggcccgctct ggctcttata taatcggctt
      421 tggctcgcat tcatcctgac ccttgtcgtt atggctggcc ttgccgcggc gctcatcgag
      481 ctggagttaa gaaaccaagc cccagcgatc gttgatcttt tggcctcgct gattatcggc
      541 tttgagggca acaatattct gcgctggggc ctgcaacgca agggctatgc ccttctggga
      601 tccgtcgtcg ggcgtagccg cctggaatgc gagcggcgct tcttcgacgc ctggcttcct
      661 cacgccgccg gaaaaggggc tgcggcagga acgcctgtga tgggttcaaa aagcggagac
      721 tggcagaccg cacacacaat cggatggccg gaggcgacgg catagcccat gtttgttgca
      781 atcgtcgatt atcaatccgg caatcttcac tcggctcaaa aggctttcga gcgcgcaact
      841 cgtgaaagcg gcctttctta cgaaatcatt gtcaccgggg atccggacgc ggtcttgcgc
      901 gcggaccgcg tcgttcttcc cggcgtcggc gcattcggcg attgctaccg cggccttagc
      961 gaaactccgg gaatgatcga cgccttgaaa gaagcagtgc tgaagcgcgg gcggcccttt
     1021 tttggcattt gcgtaggcat gcaaatgctt gccgatcgcg ggctggagca cggcacgcat
     1081 cagggcctcg gctggattgg cggcgaggtg gaccttatca agccatccga taagtcgctc
     1141 aagattcccc atatgggctg gaatacgctg tccttcgcaa gaagccacgc cctgaccgaa
     1201 ggtattcccg atggtccgga cggctggcat gcatacttcg tgcattctta ccaattcctg
     1261 cccgaagatg gcgacgatgt gcttgcgaga tcggattacg gcggcccgat cacagcgctt
     1321 gtcgcgcgcg gcaacatcgc cggaagccag ttccatccgg aaaagagcca gaaactaggg
     1381 ctgaagctaa tctccaactt tttgcagtgg cggccctgag ctcggttttc gggcctctct
     1441 cacgccggcc tctctctcat cggtgagctt tttcctgtct tgattagccg tacggcgcac
     1501 aatctgggcg atcatgacaa gcggtgaagg ctatcgctaa cggcacatta ttgtggcaca
     1561 ggttgtggcc tgatgctcgc gaagtaaatc gtgcgcgagt tccccttcat tcgttggcga
     1621 aatcgacgcg gtctgtcagc tgagacaacg ttgtgcgttg cagcaagctg cggctgggac
     1681 tacgcttggc aagcgtccag caattcaatt ggggataaat cgatgatcct ctacccggct
     1741 atcgacctga agaatggtca gtgtgtgcgc ctgcgccaag gccttatgga tcaggctacg
     1801 gttttcaacg agagcccggc cgaccaggcg taccaattcg cgcaggaggg gttttccgct
     1861 cttcacgttg tcgatctcga cggcgccttt gaggggcgct ccgtcaacgc caaagcagtt
     1921 tcatcgatcc tcgcttcagt atccattccc gtccaactcg gcggcggcat ccgcacgctt
     1981 gaagccgttt cgctctggct cagccgcggg gtgtctcgcg tcatcttggg gactgtcgcg
     2041 gtgcgcaatc cctcgctggt caaggccgcc tgcgcgcgct ttccgggcaa gattgttgtg
     2101 ggaatcgatg ccaaggatgg ccgggtggcc gtggagggtt gggcagagac ttcggagcta
     2161 tacgccaccg atcttgcacg ccggctgcaa gatgtggggg tcgcagccat catctacacc
     2221 gacatcgccc gcgacggcat gctgacgggc ctcaaccttg acgcgacagc cgatcttgcg
     2281 cgatcggtct cgatcccggt tatcgcgtca ggaggtttgg ccggcatcga ggacgtaaag
     2341 cggctgattt ccccggccta cgcgatcatc gctggtgcca tcgcgggccg ggcgctctat
     2401 gatgggcgtc tcgacgccaa gcttgcactt caactcatcg gcaagggaca tgaatagcgt
     2461 gccacgacgc cggtcgctgg cccgatcgcg gcactttttt aataaattag cgatattcac
     2521 acaattggag tgcttgttaa tcgcgttcgc cgcttcgtcc tgcgtgacat tcgtaacaca
     2581 taattgacga aatccgcctc taccggcaag tcagtgacaa gctcgggctg cgaaaggaaa
     2641 atcctcgctg atggttaagc ctccgttgct tagcggcaac agggtcaatc ccgctgctgc
     2701 aattctcctt tcattgatac tttcagctgg cctttatgca ccccgagctc atggcgaagg
     2761 cgacgatgcc gcgccgactg ctcccacctc cgatactatg aagcctgccg aaaaagcgcc
     2821 cgaagagagc aaaaagacac ccgaggaaag caagaagcag gcggaggagt cgccgagcgg
     2881 aaagcctgaa agtacagcgg aagagcccaa agaagccaag cctcaggcac cggttgcggg
     2941 gtccaagcct gcgctcgcaa agccagcgga agcaagcaaa cccgcaaaac cagccgaacc
     3001 caaagaaacc agggccgaaa agccgaaaga ccttactccc aaaccagacg aagccggcga
     3061 tctggccgtg cgcgcctcca cggtcgacgc cgtctatcaa atcagctggc ttggcacaca
     3121 tatcggcgat ttcaggatca agtcctcgat cacgacgcgg caatattctc tgcaggccaa
     3181 cgcggaactc agcgtgttct tcggcacttt cagctggcaa ggcgtaacca caagctcggg
     3241 gctgatgacg gccaac
//
LOCUS       DATMGX010000103         3469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_250628, whole genome shotgun sequence.
ACCESSION   DATMGX010000103 DATMGX010000000
VERSION     DATMGX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250628"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..2574)
                     /locus_tag="VNR65_01690"
     CDS             complement(178..2574)
                     /locus_tag="VNR65_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ17416.1"
                     /translation="MAFAHWNPHWNSVAFGLGAAGAIALSAAGYGWVALALLAGLGAA
                     ASVLFAAGQLHLKPDHGSDERIQRLFGALPAAVAVTNGRGLVQWSSEAFLNAVDGLGD
                     DHDLSRLGDRHPEAAAAIFRLFAAGSAGKAHSEVLHIRNSHSVDPMLLKVAPLVEQGK
                     RSSLLVWQIENAKKTVHGEKPKAGLDMLPVPALSVGKDLTVQGANKGFEKLAGCEPTG
                     KQAWSLFRTRRGNPLTRARLRDMIARPGSAIPVQIISASGYARSASLHLGADINEGQT
                     WIVTPADPAAGSLEESFEALLARAPTAMAFTNARGIVQAANDEFRRLFGETSEEGSAV
                     ADFSGKSLASLVAETSSQNVRDKITALSASGLAASGPVELQAVLKGSLNRRVRIVVFP
                     AANDNNLVVTAVETGAAPVIDEQAVQGQKLQAVGELAGGIAHDFNNLLTAIIGFSDLL
                     LRRFRASDPAFKDLMNIKNNATRAAELVKQILAYSRKQTLRPAILKITDVIEEFQATM
                     GRTLGEKVKAKIQHGRDLWFVKADEGQLFQVIMNLAVNARDAMPDGGEIVITTENVSE
                     RESLALKDRGLDRGEYVTCEVRDSGTGIKPEHLEKIFDPFFSTKEVGKGTGLGLSTVY
                     GIVKQTGGTILVDSEMDQGTSFRIYLPRYIETEQDLKTLETKAEAPDKVVDLTGQGTV
                     LLVEDEDAVRSFASRALATRGYKVLEAASGVEALEVMDRENGKIDLVVSDVVMPEMDG
                     PTLLRHLRQRNPDIRIIFMSGYAEEAFRKNLSADENFVFLPKPFTLKKLAETVKAAAA
                     "
     gene            2793..3257
                     /locus_tag="VNR65_01695"
     CDS             2793..3257
                     /locus_tag="VNR65_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17417.1"
                     /translation="MGSAGFIIAGIVVLALVAAGVGFYLYQRKGNGDTARLFAPRSRR
                     LAYVERVALEGGRKLLLVRRDDVEHLILIGGPIDLVVESGIHPQAVASSFVKDVSSAP
                     VAFAPSVWPLADPLPDVEPAVAPEPQLPVFPKATVGVNTPVLVSITEAKAAE"
BASE COUNT          637 a         1025 c         1068 g          739 t
ORIGIN      
        1 cccgccggtc tactgtggtg aggggcggcc acggacacgg ctgcgctggc ggccggcgct
       61 gcgtaggcgg ttaccaccat ggtgtttgcc gcagctgggc agtctgccgc tagatcaagg
      121 ccaattattg ggcagccgtc aggcgcgtgg ccttacggcc gcctcatata acggggccta
      181 agccgccgct gctttcaccg tctcagccag ctttttcagc gtgaaaggct tcggcaggaa
      241 gacgaagttc tcgtcggcgc tcaagttctt gcggaacgcc tcctccgcgt agccggacat
      301 gaaaatgata cggatatcgg gattgcgctg ccgcaggtgg cgcaaaaggg tggggccatc
      361 catctcgggc atgacgacat cgctgacgac aagatcgatc ttgccattct cgcggtccat
      421 aacttcgagc gcctcgacgc cgctggcggc ttccaacact ttgtagccgc gcgtggcaag
      481 cgctctcgat gcgaatgagc gtacagcgtc ctcgtcctcg accagaagaa cagtcccctg
      541 acctgtcaag tccacgacct tatcaggtgc ttcggctttg gtttcgagcg tcttgaggtc
      601 ctgctctgtc tcgatatagc gcgggagata aatgcggaaa gaggtgccct ggtccatctc
      661 gctatcgacg aggatggtgc ccccggtctg tttcacgatg ccgtagacgg tggagaggcc
      721 aaggcccgtt cctttgccca cttcctttgt cgagaaaaac gggtcgaaga tcttctcaag
      781 atgctcgggc ttgataccgg tgccgctatc gcgcacctcg cacgtgacat attccccccg
      841 gtcgagccca cggtccttca gggcaagcga ttcacgctcg ctgacattct ccgttgtaat
      901 cacgatttcg ccgccgtccg gcatcgcgtc gcgcgcgttc acggcgaggt tcatgatcac
      961 ttgaaacagc tgcccctcgt cggcttttac gaaccaaaga tcgcgcccgt gttggatctt
     1021 ggccttgacc ttttccccaa gcgtccggcc catggttgcc tggaattcct cgatcacatc
     1081 cgtgattttg aggatggcgg ggcgcaaggt ctgcttgcgg gaataggcaa ggatttgctt
     1141 gacgagttcc gccgccctcg tcgcgttgtt cttgatgttc atcaagtcct tgaaggccgg
     1201 gtcggaggcg cggaagcggc gcaggagcag gtcggaaaag cctatgatgg cggtcagaag
     1261 gttattgaaa tcgtgagcga taccgcccgc cagttcgccg accgcctgga gcttctgtcc
     1321 ttgcaccgcc tgttcgtcaa tcaccggggc cgcccctgtc tcgaccgccg tgacgacgag
     1381 attattgtcg tttgcggccg ggaagacgac gatgcgaacg cgccggttta gcgatccctt
     1441 gagaaccgct tgcagttcca caggtcctga agccgcgagg ccgctcgcgg aaagcgccgt
     1501 gatcttgtcg cggacattct gtgatgaggt ctccgccaca agcgacgcaa gcgacttgcc
     1561 gctaaagtct gcgacggcag aaccttcttc cgaggtttcg ccgaaaaggc ggcggaattc
     1621 atcatttgcc gcctggacga tccccctggc attcgtgaag gccatggcgg ttggcgcacg
     1681 cgcaagaagg gcctcgaagg actcttcaag gctccccgcg gcggggtcgg cgggcgtgac
     1741 aatccaggtt tgcccttcgt tgatgtccgc tccgaggtgc agggatgccg atcgggcata
     1801 accgctcgca ctgatgatct gcacggggat tgcgctgcct ggtcttgcga tcatgtcgcg
     1861 aagcctggct cgggtcagcg gatttcctcg cctcgtgcga aaaagcgacc acgcctgctt
     1921 gccggtgggt tcacatcctg ccagcttctc gaagcccttg ttcgcaccct ggacagtcag
     1981 gtctttgcca accgaaagag ctggaacagg gagcatgtcg aggcctgcct tcggcttctc
     2041 cccatgaacc gtctttttcg cgttttcgat ctgccatacc aggagcgacg agcgttttcc
     2101 ctgctcgacc aggggcgcca ccttgagcag catagggtcg acgctgtggg aattccgaat
     2161 atgaaggact tccgaatgcg ccttgccagc agagcctgcg gcgaaaagcc ggaaaatggc
     2221 cgccgcggcc tctggatgcc ggtcaccgag ccgggaaaga tcatgatcgt cgccaaggcc
     2281 atccacagcg ttcagaaatg cttcgctgct ccattggacc aagcccctgc cgttggttac
     2341 ggcgaccgcg gccggtagag cgccgaaaag gcgctgaatg cgctcatcgg agccgtgatc
     2401 cggcttcaga tgcagttgtc ccgccgcaaa cagcacggac gccgccgcgc cgagacccgc
     2461 cagaagcgca agcgcgaccc acccatatcc cgcggcggac agcgcaattg cgccagccgc
     2521 gccgagccca aacgcgacac tattccaatg cggattccag tgtgcgaaag ccattgactg
     2581 caccgccatg ttttgaaacc tcgctcttgg ccgcgactat gagatgattc gccttacgct
     2641 gggacccggg cgcgtaaagc aatctcgccg ctgggaaagt taaaaatgtc ttgcgaagtt
     2701 gcgaccggtc gcatcttttg ccccaaggca taggcatttc gttgtcgttc ggcgtggttg
     2761 agcgttcggg actttgacaa aagggggatt gaatgggctc cgcaggcttt atcattgccg
     2821 gaatcgtcgt tcttgcattg gttgctgctg gcgtcggctt ctacctatat cagcgaaaag
     2881 gtaatgggga tacggcacgg ctgtttgcac cgcgttcccg gaggctggcg tatgttgaac
     2941 gggtcgcgct tgaagggggc cggaagctcc tcctggtccg gcgggatgat gtcgagcatc
     3001 ttatcctgat aggggggcct atcgatctcg tcgtcgagtc gggcatacac ccgcaggcgg
     3061 ttgccagttc tttcgtgaag gacgtgagtt cagcgccggt ggcgtttgcg ccttcggtgt
     3121 ggccgctcgc cgatcccttg cctgacgtgg aaccggctgt ggcgccagag ccacagttgc
     3181 ctgttttccc caaggcaacg gttggggtaa atacgcctgt tctcgtctct ataacggaag
     3241 ccaaggcggc ggagtagaac cacaaggtgc tgattagatt aggggacgta attttgtggg
     3301 cgcagatccg gggcgagcct tatgtctatc tggggtaagc tgacaggcgc ggccgcgggg
     3361 ctggctgtcg gtgggccgat cggcgcgctt atcggcggcg tcgcagggca catcgctatc
     3421 gatcatggga tcggttcaaa ctcgaatcac gacaagcaag ttgccttca
//
LOCUS       DATMGX010000104         3955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_444862, whole genome shotgun sequence.
ACCESSION   DATMGX010000104 DATMGX010000000
VERSION     DATMGX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444862"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..742)
                     /gene="fabA"
                     /locus_tag="VNR65_01700"
     CDS             complement(209..742)
                     /gene="fabA"
                     /locus_tag="VNR65_01700"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF003509.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HWJ17418.1"
                     /translation="MMTERRHSYDYKDLLACGRGELFGAGNAQLPLPPMLMFDRITSI
                     SETDGEHGRGRIVAEMDVRPDLWFFACHFKGDPVMPGCLGLDALWQMLGFFLGWTGAP
                     GKGRALSVGEVKFSGMVVPSVKRLEYIVDLKRVIRRKFTLGVGDGQLKADGTLIYSAK
                     ELRVGLFAADMSMQAAG"
     gene            922..1338
                     /locus_tag="VNR65_01705"
     CDS             922..1338
                     /locus_tag="VNR65_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWJ17419.1"
                     /translation="MPDNVPELPSERLRMTGLRPTRQRVALAELLFGRGDRHFTAETV
                     HAEAVKNGVPVSLATVYNTLHQFVEAGLLREVAIEGSKTYFDTKISNHFHFFFEKDGT
                     LVDIEPANIKVENLPKPPEGTAIARIDVLVRLAPIK"
     gene            1377..1772
                     /locus_tag="VNR65_01710"
     CDS             1377..1772
                     /locus_tag="VNR65_01710"
                     /inference="COORDINATES: protein motif:HMM:NF021510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HWJ17420.1"
                     /translation="MLDRLEEEQQPQKNVFGEPLAACSDNPLTGFFRDGCCSTGPQDY
                     GSHTVCTIMTEEFLAFSKAAGNDLSTPVPEFAFPGLKAGDRWCLCAARWQQAFEAGKA
                     PRVVLAATHERALEIVALKDLKRFAIDLS"
     gene            complement(1785..2948)
                     /locus_tag="VNR65_01715"
     CDS             complement(1785..2948)
                     /locus_tag="VNR65_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWJ17421.1"
                     /translation="MQFRRVCVILGALAFCLALFADARAATLIDKSPSSTAAVRESLL
                     AAEFATKGLKLGSPVFLRVYKQSSTMELWVAQGARYALFKTFKICRWSGGLGPKMFEG
                     DRQSPEGLYHITAEDLIVNPRWHRAMNINFPNRFDVINGRGGSGILIHGKCGSVGCFA
                     IQDNNVEEVYDAVRAALHKGQVSIPVLALPFSFAKYAPAVEDTLKLNEFWSDLRRADI
                     LFNRDRVPPMAWVCDGRYYFGDRRGDSHRHAVHLPGCKPLDKPITQQEAASLSEESQS
                     ILAAATARNKWLVSGAGETSKSVIPAINAQENPRQAVQNFANACNPKKLDCRLLREAL
                     KSPAMCPTKYARCRTAQATYTKSVECPLKYPRCRSFVGKEPRIASKSNKTKQR"
     gene            3372..>3955
                     /locus_tag="VNR65_01720"
     CDS             3372..>3955
                     /locus_tag="VNR65_01720"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469332.1"
                     /note="NADP-dependent; catalyzes the oxidative
                     decarboxylation of malate to form pyruvate; decarboxylates
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HWJ17422.1"
                     /translation="MANEFKQSAIEYHEADPPGKLAITPTKPLATQRDLSLAYSPGVA
                     FACEAIVNDNAAAARLTARANLVGVVTNGTAVLGLGNIGPLASKPVMEGKAVLFKKFA
                     GIDVFDIEVAATDPDRFVDAVAALEPTFGAINLEDIKAPECFEIEHKLRERMKIPVMH
                     DDQHGTAIVVAAAILNGLKIVGKDIGDVRLACCGG"
BASE COUNT          828 a         1122 c         1144 g          861 t
ORIGIN      
        1 ggttctccgt gaacctggca gcggaagccc ctttcggcgt aatctttcgc cgtgacgagg
       61 ccggaccgcg cttcacgcaa ggaggcaagc acctcctggc tgttgttacc gatggacgag
      121 acgatgccca ttcccgtgac agcaacacgt ctcatgtttt aaccccacgt tgtgtcgcac
      181 tagattctct aaaagctggt agttcagctc agccggcggc ttgcatgctc atatcggcgg
      241 cgaacaggcc tacccggagc tctttcgcag aatagatgag cgtgccgtcc gccttgagtt
      301 gtccatcgcc aacgccaagc gtgaatttcc ggcggataac cctcttgaga tccacgatat
      361 attccagccg cttgacgctt ggcaccacca tgccgctaaa cttcacttcg ccgacgctta
      421 gcgcgcgccc tttgccagga gcgccagtcc acccaagaaa gaagccgagc atttgccaaa
      481 gcgcgtcgag cccaaggcaa ccaggcatga cgggatcgcc tttgaaatgg caagcgaaaa
      541 accaaaggtc gggacgcacg tccatctcgg ccacgatcct gccgcgcccg tgctccccgt
      601 cggtttcgga aatgctcgta atgcggtcga acatcagcat cggcggaagc ggcaattgcg
      661 cgttccctgc tccgaatagt tcgccgcgcc cgcaagcgag caaatccttg tagtcgtagc
      721 tatggcgccg ctcagtcatc attcccctat attctctcat tacgcctgcg gtcgggcaag
      781 gcgttccctt aattatcaca ccttgcggcg ctcgcaacct gtctccaatt ttctttgcga
      841 tgggctcgag gggacagcgc ttgcgagtca agactccgat ccttatacta caaggctaga
      901 gccaaggaaa tgctgagaga aatgcccgac aacgttcccg aattgccaag cgagcggctc
      961 cgcatgacag ggttgcgccc gacgcggcaa cgcgtcgcgc tcgcggagct tctgttcggc
     1021 cgcggggaca ggcactttac ggcggagacg gtgcatgcgg aggcggtgaa gaacggcgtg
     1081 cccgtgtccc ttgcaacggt ctacaatacg cttcaccaat tcgtcgaagc cggtctcctg
     1141 cgggaggtcg ccatcgaagg ttcgaagacc tacttcgata ccaagatatc caatcacttt
     1201 cacttcttct tcgagaagga tggaacactg gtcgatatcg agccggccaa catcaaggta
     1261 gaaaatctgc cgaagccgcc tgaaggcacg gcgatcgcgc ggatcgacgt gttggtgagg
     1321 cttgcgccga tcaagtaaat ttacacgcct tttacgcaaa atctggagcg aatgtcttgt
     1381 tggataggct tgaagaggag caacagcctc agaagaacgt cttcggggag cctttggcgg
     1441 cgtgctccga caatcccctt accggatttt ttcgtgacgg ctgctgcagc accggtccgc
     1501 aggattacgg ctcccacacg gtctgcacaa ttatgaccga agagttcctg gccttctcga
     1561 aggcggcggg gaacgatctt tcgacccccg ttccggaatt tgcgtttccg ggccttaagg
     1621 caggggaccg gtggtgttta tgcgcggcgc ggtggcagca ggctttcgaa gctggtaagg
     1681 cgcctcgggt tgtgcttgct gcgacgcatg aacgcgcttt ggagatcgtc gcgttgaagg
     1741 atttgaagcg cttcgcgatc gatctctctt gagcctggtg aggcttagcg ctgctttgtc
     1801 ttgttggact tgcttgcgat gcgtggctcc ttgcccacga aagagcggca gcgcggatat
     1861 ttaagcgggc attccacgct tttcgtgtag gtggcttggg ctgtgcggca gcgggcgtat
     1921 ttggtcgggc acatggctgg gctcttcaac gcctcacgca gcagacggca atcaagcttc
     1981 ttcggattgc aagcgttggc gaaattctgg acagcctgtc tggggttttc ctgcgcattt
     2041 atcgccggta tcacggactt gctagtttcg cctgcgccgg agaccagcca cttgtttcgc
     2101 gccgtggctg cggcaaggat ggattggctc tcttccgaaa ggcttgccgc ttcttgctgc
     2161 gtgatgggct tatcgagcgg tttgcagccg ggcaggtgaa cagcatgacg atggctgtct
     2221 ccgcgccggt ccccgaaata ataacggcca tcgcagaccc atgccatcgg agggacgcgg
     2281 tcgcggttga agagaatgtc cgcgcggcga agatcggacc agaactcgtt caatttcagc
     2341 gtgtcttcaa cggctggtgc atattttgcg aagctgaaag gcagtgcaag gacgggaatg
     2401 ctgacctggc ctttatgaag cgcggcgcgc accgcgtcgt aaacttcctc gacgttgttg
     2461 tcctgaatgg cgaagcatcc gaccgaaccg catttgccat ggatgaggat gcccgaaccg
     2521 ccccggccgt tgatgacatc gaaccggttc ggaaagttga tgttcattgc gcgatgccag
     2581 cgcggattga cgatgagatc ttcggcggtg atgtgataca gcccttccgg cgactgccgg
     2641 tcgccttcga acatcttcgg gcccaaccca cctgaccagc ggcaaatctt aaaggtcttg
     2701 aacaaggcat aacgcgcacc ctgcgcaacc cacagctcca tcgtcgaact ctgcttgtaa
     2761 acgcgcagga agacagggga cccaagtttc aggccttttg tggcgaattc tgctgctagg
     2821 agactttctc ttacagcggc ggtcgagctt ggcgatttat cgataagggt tgccgctctg
     2881 gcgtccgcga agagcgcgag gcaaaaggcg agcgctccca gtattacgca gactctacga
     2941 aactgcatct tcactcctgt aactacgcgt acatgcatga aactctggca aggcaaggtg
     3001 ctctcgcgtt gttaacgaaa aagtaaccgc ccggcataca tttccagaga aaaattgtga
     3061 tgttaatatg acacaagctg gacagatggt cgcaataatc gtgatatatt tttacgaaaa
     3121 ttgtctatta atgcgaaaca atggatctaa ttcatccatt tgaatgagag gaattgcctt
     3181 tgtgccgcct tcggcgcgac acgcttgtaa tggcgctgaa gtcaccggta agtgcctaat
     3241 tctattcgcc tcgggaaagt gttcctctct gttttctgtt ccagagcctc atttttcaac
     3301 taaacgtcag attgacaggc acagatagac cggccttcct cttttgtagg gaagccgcag
     3361 gagagagcgc tatggcgaac gagttcaagc aaagcgccat cgagtatcac gaggccgatc
     3421 cccctggtaa gctcgccata acgccgacaa aaccgcttgc gacgcagcga gatctgtcgc
     3481 tcgcttattc gcccggcgtc gccttcgctt gcgaggcaat cgtaaacgac aatgcggcgg
     3541 cggcgcggct gaccgcgcgc gcaaaccttg tcggcgtcgt cacgaacggg accgcggttc
     3601 tgggccttgg caacatcggg ccgctcgcct cgaagccggt gatggaaggc aaggcggttc
     3661 ttttcaagaa attcgcaggg atcgacgttt tcgacatcga ggtggccgcc accgaccccg
     3721 accggtttgt cgacgcggtc gccgcccttg agccgacctt cggcgcgatc aacctcgagg
     3781 atatcaaggc cccggagtgc ttcgagatcg agcacaagct tcgggagcga atgaagatcc
     3841 ccgtcatgca cgacgatcag catggcaccg cgattgtcgt ggcggcggcc atcctgaacg
     3901 gcctgaagat cgtcgggaag gacatcggcg atgtaaggct tgcctgctgc ggggg
//
LOCUS       DATMGX010000105         2757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_276013, whole genome shotgun sequence.
ACCESSION   DATMGX010000105 DATMGX010000000
VERSION     DATMGX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276013"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /gene="glgP"
                     /locus_tag="VNR65_01725"
     CDS             <1..881
                     /gene="glgP"
                     /locus_tag="VNR65_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWJ17423.1"
                     /translation="IREWTHFIRVSGARQHAIFLADYDMLLSEHLVQGVDVWINTPRR
                     PWEASGTSGMKVLVNGGINLSELDGWWAEAYTPEVGWALGDGQEHGDDPAWDAAEAET
                     LYDLLEREVIPEFYARDGQGIPTAWVTRMRSSMATLTPRFSADRTVREYTEQHYLPGA
                     ATYRERAADKGAVGRQMVEWQHAIQQAWPALRFGEMKVETNGDRHKFEVQVYLGELDP
                     SAVRIELYADGVNGDGPERQEMERARQLPGGAGGYAYRAPVPAMRPATDYTARVIPQH
                     TGVAVPLEDERILWQR"
     gene            906..2321
                     /gene="gndA"
                     /locus_tag="VNR65_01730"
     CDS             906..2321
                     /gene="gndA"
                     /locus_tag="VNR65_01730"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HWJ17424.1"
                     /translation="METERHEIGMIGLGVMGCNMLLNMADHGFPVAGYDKDPGKVEAL
                     QKESAEHDIHSAANIKDFIGLLRRPRAIMMLVPAGPPVDSVIADLLPHLETGDLIIDA
                     GNSYFKDTNLRASNLAAKGIQFLGVGVSGGEEGARHGASIMPGGPEEAYERVRPLLEA
                     VAAKVNGDACVAYLGPGSAGHFVKMVHNGIEYAVMQLLAETYDLMKRGLGLSDDELRE
                     VYGTWNQGELNGYLLEITSRIFGKVDERTGKRLIDEILDVAKQKGTGMWTSQSAMELQ
                     VPIPTIDLAVAMRDLSVFEKQRETAGEIFHRPIPPFAGDRKAFLDKIGQALHAAMIVV
                     YAQGMAVLAAASDTYKYHLDLETVARIWRGGCIIRAVLLDDIRMAYRNQPGLPNLLLA
                     PELSIKVIAHEEDLRRVVCAAIELGLPAPCFMTALAYLDGYRSAWLPANLIQAQRDYF
                     GAHSYERIDVKGTFHTDWTKG"
     gene            2328..>2757
                     /locus_tag="VNR65_01735"
     CDS             2328..>2757
                     /locus_tag="VNR65_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17425.1"
                     /translation="MQTNDQLAPNIFVIFGGAGDLTWRKLMPALFDLSQSHSIPVHFS
                     IIAVDRVALSVDVLRKRLHEGVRKFARSGKAPVKEWKEFSPHIRYLQGDFKSNATYKE
                     LSDQCANLEKEWGAKAQRIFYMATPPSLFGEIPSFLNYAGL"
BASE COUNT          568 a          831 c          876 g          482 t
ORIGIN      
        1 tgattcggga gtggacacat ttcatccggg tatccggggc gcgtcagcat gccatcttcc
       61 tggctgatta cgacatgctc ctgagcgagc acctggtgca aggcgtggac gtctggatca
      121 acacgccgcg ccggccctgg gaagccagcg ggaccagcgg catgaaggtg ctggtcaatg
      181 gaggcatcaa tctgtcggag ctggacggct ggtgggcgga ggcctacacg cctgaagtgg
      241 ggtgggcgct gggggacggc caggaacacg gcgacgatcc ggcctgggac gcagccgaag
      301 ccgaaacgct ctacgacctg ctggaaaggg aagtgattcc cgagttttat gcccgcgacg
      361 ggcagggtat ccccacggct tgggtgacgc ggatgcgaag cagcatggcg actttgacgc
      421 cgcgcttctc cgcggaccgc acggttcgcg aatacacgga gcaacactac cttccaggcg
      481 ccgcaaccta ccgcgagcgt gccgccgata aaggcgcggt gggccggcaa atggtcgagt
      541 ggcagcacgc catacaacag gcgtggccag cgctgcgctt cggtgaaatg aaagtcgaga
      601 ccaacggaga ccggcacaag tttgaagtgc aggtctatct cggtgaactc gacccgagtg
      661 cggtgcgcat agagctttat gccgacggcg tcaacggcga cggtcccgaa cggcaggaga
      721 tggagcgcgc tcgccaattg cctggtgggg ccggaggcta tgcctatcgc gcgccggtgc
      781 ccgcgatgcg tccggccacc gactacacgg cgcgcgtgat accgcaacat accggcgttg
      841 cggtcccgct ggaagacgag cgaatcctgt ggcagcgatg atccgacttc gaatggagaa
      901 ctggtatgga aactgagcgc catgaaattg gcatgatcgg cttgggcgtg atggggtgca
      961 atatgttact gaacatggcc gaccacggct tcccggtggc gggctatgac aaggatcccg
     1021 gcaaggtcga agcgctccag aaggaatctg cggaacacga catacacagc gcggccaaca
     1081 tcaaggattt catcggcctg ctccgccggc cgcgcgccat catgatgctg gtgcccgccg
     1141 gcccgcccgt ggattccgtg atcgccgatc tgttaccgca cctcgaaacg ggcgatctca
     1201 tcatcgacgc cggcaactcc tacttcaaag ataccaacct gcgtgccagt aacctggcag
     1261 cgaagggcat tcagttcctc ggtgtgggcg tgtctggcgg cgaggaaggc gcgcgccacg
     1321 gcgcgagcat catgcccgga gggcccgagg aggcgtacga gcgggtccgc ccgctgctcg
     1381 aggccgtcgc cgccaaagtc aacggagacg cgtgcgtagc gtacctcggt cccggatcgg
     1441 ccgggcattt cgtcaagatg gtccacaacg gcatcgagta cgcagtgatg caacttctgg
     1501 ccgagacata cgacctgatg aaacgcggcc ttggcttgag cgacgacgaa ctgcgcgaag
     1561 tgtacggcac gtggaatcaa ggggaactca acggctacct cctggagatc accagtcgca
     1621 tctttggcaa agtcgacgag aggaccggca agcgattgat cgatgaaatc ctggatgtgg
     1681 ccaaacagaa aggcaccggg atgtggacct cgcaaagcgc gatggaactg caagtgccca
     1741 tccccacgat cgacctcgcg gtcgcgatgc gggatttgtc cgtgtttgaa aagcagcgcg
     1801 agacggccgg cgagatcttc catcggccca tccccccgtt tgccggcgac cgcaaagcct
     1861 tcctcgacaa gatcggccaa gcgctccatg cagcgatgat tgtcgtctac gcacagggga
     1921 tggcagtcct ggcggcagcg tcggacacgt acaagtatca cctcgatctc gagaccgtcg
     1981 cgcgaatctg gcggggcggc tgcattatcc gcgcggtgtt gctggatgac attcggatgg
     2041 catatcgcaa ccagcccggc ctgccgaatc tgctcctggc cccggagttg tcgatcaagg
     2101 tcatcgccca cgaggaggat ttgcgccgcg tcgtctgcgc ggcaatcgag ctgggtttgc
     2161 ccgctccctg cttcatgacg gcgctcgcct accttgacgg ctaccgcagt gcctggcttc
     2221 cggccaacct gatacaggcc cagcgcgact acttcggtgc gcacagctat gagcgcatcg
     2281 acgtcaaagg cacattccac actgactgga cgaaaggttg agaggacatg cagaccaacg
     2341 atcagcttgc gccaaacata ttcgtcatct tcggcggagc gggcgacctt acctggcgga
     2401 agttgatgcc ggccctgttt gacctttccc agagccacag cattccggtt catttttcga
     2461 ttatcgcggt ggaccgggtc gctctcagcg tggacgtcct tcgcaagcgc ctgcatgagg
     2521 gagtcaggaa attcgcccgc agcgggaaag ccccggtcaa ggagtggaag gagttctccc
     2581 cgcatatccg ctacttacag ggcgacttca agagcaacgc gacctacaag gagcttagcg
     2641 accagtgcgc aaacctggag aaggagtggg gcgccaaagc ccaacgcatt ttctacatgg
     2701 ccacgccgcc ctccctgttt ggcgaaatcc cgtcctttct aaactatgcc ggcctgt
//
LOCUS       DATMGX010000106         3077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_151032, whole genome shotgun sequence.
ACCESSION   DATMGX010000106 DATMGX010000000
VERSION     DATMGX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151032"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VNR65_01740"
     CDS             <1..303
                     /locus_tag="VNR65_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWJ17426.1"
                     /translation="GEAPYDGAAVQKALTDVNKSLATFVTLFPKGSETGGETAAKPEI
                     WEDKADFEAKAKDLEAATAKAAAAAPGGLDAFKVAFGPVGKACKACHEKYREEKKE"
     gene            311..1219
                     /locus_tag="VNR65_01745"
     CDS             311..1219
                     /locus_tag="VNR65_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWJ17427.1"
                     /translation="MIAVRAFLLLSCLFAWASDPIVSAQASNVENGRYLFYAGGCASC
                     HAAPASAKCDDRNYKDPLTLTGGRCMKSAFGTFYVPNISQDKQSGIGGWSDDDFIRAM
                     TEGVSPTGKHYYPAFPYASYRHMARDDLLDLKAFLNTLPAAPSKAPQHELAFPYNIRW
                     GLGLWKEFNLNGGTFKPDPSKSAEINRGAYLVEGPGHCGECHTPRNWLGGTIEGKKLS
                     GAPNPEGKGFVPNITPDGTGLTSWSHKDIVYALETGFTPEGDVLGGSMAKVQENMAKL
                     TPGDREAIAAYLKSVPPIANERPKKK"
     gene            complement(1257..1655)
                     /locus_tag="VNR65_01750"
     CDS             complement(1257..1655)
                     /locus_tag="VNR65_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17428.1"
                     /translation="MTVHDMIEDREAPLVLQRKDLAQLIMLQMNPVPKLTLYREIATA
                     ILRMEAVMRQSAAQAPQEQKPEPILTPATLHHWEDLRQWVHSSVEAASVGLAGFRAAM
                     PVLHAPRLSLLALSGLRYYRPSGFRAAIST"
     gene            2029..2814
                     /locus_tag="VNR65_01755"
     CDS             2029..2814
                     /locus_tag="VNR65_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWJ17429.1"
                     /translation="MVNSLQTAVLQFPVSMDAGRNLERLTACIDQLDAGTLAVAPEGA
                     LSGYAPEPGFVASLDPGAIARAIDSARSTVANKKIHLVVGACFLDDGAWKNSSFYIGP
                     EGRLERYDKINLAQSERGTFHPGNRLPVFEIDHEGEAFHLGIQMCREIRYPEQWRVLA
                     AKGAQVIAYVNNAVGSRDGDHVWRAHMISRAAETQRFILGANNAASDQKCPSMIVSPS
                     GKVLAEIAIGSEGKAEATLVLSEVSDWVLSQARDDVVAVLLRE"
BASE COUNT          691 a          901 c          889 g          596 t
ORIGIN      
        1 ggcgaggcac cgtatgacgg cgcggccgtc caaaaggcgt tgacggacgt caacaagtcg
       61 cttgcaacat tcgtcactct gttcccgaaa gggtccgaaa ccggtggcga aaccgccgcc
      121 aaacccgaaa tctgggagga caaagccgat tttgaggcaa aggcaaagga tctcgaggcg
      181 gctacggcca aggctgccgc tgctgcaccg ggcgggctcg acgctttcaa ggtggctttc
      241 ggccctgtcg gcaaggcctg caaggcgtgc cacgaaaagt accgtgagga aaaaaaggag
      301 tagatctgca atgattgccg tccgcgcctt cctgctcctg tcctgtttgt tcgcctgggc
      361 atccgatccg attgtcagcg cgcaggccag taacgtggag aacggacggt atctgtttta
      421 tgccggtggc tgcgcatcct gccacgcggc tccggcttcc gccaaatgcg atgatcgaaa
      481 ttacaaggat ccgctgacgc tcaccggcgg gcgctgcatg aaatcggcct tcggcacctt
      541 ctatgtaccg aacatctctc aagacaagca gtccggcatc ggcggatgga gcgacgacga
      601 cttcattcgg gcgatgaccg aaggcgtatc gcccacgggg aaacattact atcccgcttt
      661 cccctatgcg tcctaccggc acatggcgcg cgacgatctg ctcgatctga aggcgttcct
      721 gaacactctt ccggccgcgc cgtcaaaagc gccgcagcac gagcttgcgt ttccctataa
      781 tatccgctgg ggcctgggat tatggaaaga gttcaatctc aatggcggaa ccttcaagcc
      841 tgatccatcg aagagcgctg agatcaatcg cggagcctat ctcgtcgaag ggcccggcca
      901 ttgcggcgag tgccacacgc ctcgcaactg gctcggcggc acgatcgagg gcaagaagct
      961 ttcaggcgcg ccgaacccgg aaggcaaagg cttcgtcccc aatatcacgc ccgatggcac
     1021 agggctcaca tcctggagcc acaaggacat tgtctatgcg ctggagacgg gcttcacacc
     1081 cgagggcgat gtcctcggcg gctcgatggc gaaggttcag gaaaatatgg cgaaactgac
     1141 gccaggggat cgggaggcga tcgccgccta tctcaagtca gttccgccga ttgcgaatga
     1201 gcgtccgaag aagaagtagc ctgaaggcag tccgcatttg cgaccgcgcc gtttgcttat
     1261 gtgcttatgg ctgccctaaa gcctgaagga cggtaataac gcaacccact gagcgccagc
     1321 aacgacaacc ttggggcatg aaggacaggc atagcggctc ggaatccggc cagccccaca
     1381 gacgcagcct ccaccgaaga atggacccac tgccgcaaat cttcccaatg atgcagggtc
     1441 gcaggagtca ggatcggctc gggcttctgc tcttggggtg cttgcgcggc cgactgtcgc
     1501 atgaccgctt ccatcctgag gatggccgta gcgatctcgc gataaagcgt cagcttcgga
     1561 acggggttca tctgcagcat gatcagttga gcaaggtctt tccgctgaag caccagcggg
     1621 gcctctctat cctcgatcat gtcatggact gtcaaaagga cgctccgacg ctggcatgaa
     1681 actccccctg cgatgaccct ttcatcaagg taactctgca gggaacggaa cgcgttgcag
     1741 atcccacgcg ctgattgcgc aatcttaacg caggattgca cgatctcatc aaagactgga
     1801 agcaggctct cttggacgtg gctccgcctt tcgacaatag ccgcgtactg gttaaacttc
     1861 ataggaaaca ccccttttcg gctgaaactc gaaaggtaca ggaactcaat taaaaattgc
     1921 aaaaggcaga cattacaagc gactacacct cgatgaaatt agaaatatcg cccgtctgtt
     1981 tgagcggtgt atccaagggc actcaaatag aaacttgcgg acgccccaat ggtgaacagt
     2041 ttacaaaccg ctgtcctcca atttcccgtt tcaatggatg cgggccgcaa cctcgaacgc
     2101 ctgacggcct gtatcgatca actggatgcc gggacgcttg ctgttgcacc ggaaggtgcc
     2161 ctgtccggct atgcgcctga gccgggcttt gttgccagcc ttgacccagg agccatcgcg
     2221 cgcgcgatcg atagcgcccg ttcgactgtc gcgaacaaga aaatccatct cgtcgtgggt
     2281 gcctgcttcc tcgacgatgg tgcgtggaag aactccagtt tctatattgg gccggaaggg
     2341 cgccttgaaa ggtacgataa gatcaatctt gcccaaagcg agcgaggaac cttccaccct
     2401 ggaaaccggc ttcccgtatt tgaaatcgat catgagggcg aggcttttca tctcggcatc
     2461 cagatgtgcc gggagatacg ctatcctgag cagtggagag ttctggcggc taaaggcgct
     2521 caggtcatcg cttacgtcaa caatgccgtt ggcagtaggg atggtgatca cgtgtggcgc
     2581 gctcacatga tcagccgggc ggccgagacg cagcgcttta tccttggcgc aaacaatgcg
     2641 gcctcggatc agaagtgccc gagcatgatc gtctctccgt caggcaaggt tctcgccgaa
     2701 atcgctatag ggagcgaagg gaaggcagag gcgacactcg tcttaagtga agtctccgac
     2761 tgggtgctca gccaggcgcg tgatgacgtt gtggctgttc tcctgcgcga gtaacagcgc
     2821 tggtgtgacc tatgcaatga aaaaccccgg agccagggca tccggggtca tcttgttgca
     2881 actctcgacg agagttagcc tttaaggcgg gttgagctaa tagccccagc cgccgcagaa
     2941 ggagtatgag tttacgctgc agaagccggg tccatagcca gccgcgcggc aattccagta
     3001 gcagcttccg ccgccggacc cataaccgta gccgaccagc cctacgccaa cgggccagcc
     3061 ccagccgccg ccccacc
//
LOCUS       DATMGX010000107         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_444805, whole genome shotgun sequence.
ACCESSION   DATMGX010000107 DATMGX010000000
VERSION     DATMGX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444805"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..667)
                     /locus_tag="VNR65_01760"
     CDS             complement(158..667)
                     /locus_tag="VNR65_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17430.1"
                     /translation="MIEITSRPAGSGIAHMSSQPIALAAGALIVIVLGVGGIALWRTY
                     TGTAPEQDRIVVARQLQARTAQASEQLVEKTKGLEVTQQESIDQLQVVQDQLQAVRRL
                     LAAQQADTKRLSEQVSGLTEAVESLRHSFASAQGPDASAAPSPRNKSVRTRSRATRSA
                     HHKPGKSPG"
     gene            complement(817..1710)
                     /locus_tag="VNR65_01765"
     CDS             complement(817..1710)
                     /locus_tag="VNR65_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ17431.1"
                     /translation="MPSGAVATFADAALRYLETGHRKRYVARLVKHYRDTPLTDFNQE
                     MIDDGAAALFPNVLPATRNTVFYTPLSAILHHAGSTAKIRRPKDSKGRIVTDALSQED
                     AFAIIAAAESFDAEYALLLKFLLYTGVRLSEALRLTWDDIRLSEKAAMVRFSKNGEPR
                     SLLLRTDLNEALAAHKRTTNHEHVFRLRQGGWLKQKLLRAKLMALGIPMPPRPKDGEK
                     FRTPPHRLKWVNHHTFRHTWGTWMRRYGGADEIGLAQTGNWRDPRSVRRYVHAVPRDE
                     WERVEKLPSRGITVESTFSHG"
     gene            2022..>2205
                     /locus_tag="VNR65_01770"
     CDS             2022..>2205
                     /locus_tag="VNR65_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17432.1"
                     /translation="MCNSFAASAIVKVRVTRSFDGDAVLVEPLHAIGREAAPWPLKMA
                     SDDGEIRRIARILMRKW"
BASE COUNT          424 a          642 c          676 g          463 t
ORIGIN      
        1 gatattgaaa ttggcttacg ccatccggtg aactgcacaa tgccccgggt ttgcacggac
       61 atatgtgatc cagttcacaa tcggcgcaaa aaagttccgg ccgccctgtt tttagggcga
      121 agtgccatcg gcggacagca acaaacgggg tcgagcgtca gccgggcgat ttcccgggct
      181 tatgatgggc gcttctggtc gcccgcgatc tggttcggac cgacttgttg cggggtgacg
      241 gcgcggcgga tgcgtcgggg ccttgggcgc tggcgaagga atgccgcaaa ctctcgacgg
      301 cctcggtcag cccggaaacc tgctcggaga gccgcttggt atcggcctgc tgggcggcga
      361 gcaggcgtct gacggcctgc aactggtcct gaaccacctg caactggtcg atcgattcct
      421 gctgggtcac ttccagaccc ttggtcttct cgaccaactg ttcggaagcc tgcgcagtcc
      481 gggcctgcaa ttgacgggca acgacgatgc ggtcctgctc cggggccgta cccgtgtaag
      541 tgcgccatag cgcgatgccg ccgacgccga gcacgatgac gatgagggcg cctgcggcca
      601 aagcgatcgg ctgcgagctc atatgggcga tgccgctccc ggcgggccgg gatgtgattt
      661 cgatcatggg aggtcaaagc catgggaacc cgaaagttcc cggaattacc acaacttggg
      721 gtgcggcgcc cagccccaaa acagcgcgtg ctgcaccgca aaaagttgcg gccagctctt
      781 gggggagagc attcttcggt caataaaaac aatgagttac ccatgactaa atgtgctttc
      841 cacggttatt ccacgggatg gcagcttctc caccctttcc cactcatccc gggggacggc
      901 gtgaacatag cggcgaacac tgcgcggatc gcgccagttt ccggtctgtg ccagtccaat
      961 ctcgtcggcc ccaccatacc ggcgcatcca cgtcccccag gtgtgacgaa aggtgtggtg
     1021 gttcacccac ttcagcctat gcggcggcgt ccggaacttc tcgccgtcct tggggcgcgg
     1081 cggcatcggt atcccgaggg ccatcaactt ggcgcgaagt agcttttgct tgagccaacc
     1141 gccttggcgt agccgaaaga cgtgctcgtg attggtggtg cgcttgtgag ccgctagagc
     1201 ctcattcaag tccgtccgga ggagaaggga ccttggttcg ccattcttgc tgaagcgaac
     1261 catggcagcc ttctcggaca gccggatatc gtcccaggtc aatcgcagtg cctcactgag
     1321 gcggacaccc gtatagagca ggaatttcaa gagcagggcg tattcagcgt caaaagactc
     1381 ggctgcagcg ataatggcaa aggcgtcttc ctgactcagg gcgtcggtga cgatcctacc
     1441 cttagagtct ttcgggcgcc ggatcttcgc tgtgctgccc gcatgatgca ggatcgcaga
     1501 tagcggggta tagaaaacag tgttccgggt cgcggggagg acgttcggaa aaagagcagc
     1561 tgctccgtcg tcgatcattt cctggttgaa atcggtcagc ggcgtgtcgc gataatgttt
     1621 gacgagcctt gcaacgtacc gcttgcggtg tcccgtctcc agatagcgca atgctgcatc
     1681 ggcaaaagta gctacggcac cgctcggcac cgcctcccat tccccgcgct caattttgtc
     1741 ttgaaaatcc tttactgctt aatagcgact gatcgccggt cagttccgca agtcttgtta
     1801 acgtacttgc cgaggtacgt cccgcgaacc gagtagttcg ggcttttgtt cttgcggggt
     1861 tcgatgagtt tgaggggcat ggtgtgctca tggttgcgtc gagaatacgg gcaatgtcgg
     1921 tttcgcgaaa aactttggtc ctgccgagct tgctgaagta cggcaacccc cgagcgtcta
     1981 ctggatggtc acggagccag tcctgaagcc agcgcctgga cttgtgcaac tccttcgcgg
     2041 cctcggccat cgtaaaggtg cgcgtcaccc gctcctttga tggtgacgcc gtcttggtcg
     2101 aaccactcca tgccatcgga cgcgaagccg caccatggcc tttaaagatg gcgtccgacg
     2161 acggcgagat caggcggata gcccggatcc tcatgcgcaa atggt
//
LOCUS       DATMGX010000108        10688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_444368, whole genome shotgun sequence.
ACCESSION   DATMGX010000108 DATMGX010000000
VERSION     DATMGX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444368"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VNR65_01775"
     CDS             complement(<1..578)
                     /locus_tag="VNR65_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17433.1"
                     /translation="MTRRDNPPGGRPWRAGGGFYGCTDKQQQPDLTASQPENPTLIEP
                     VEAALEPQEQPPITRARCWEDYDRKAVSCDRDELASELNHELAGEVNRAIKWIDQSDP
                     DQLDWYLDCHGELFNVLDVVFLLMKEMDVTDTEARAIASATRKHVRRAMGRWRLHQLE
                     LMEAALSFYDAEPSPDPEPPPKRKPASWLTWR"
     gene            1023..1166
                     /locus_tag="VNR65_01780"
                     /pseudo
     CDS             1023..1166
                     /locus_tag="VNR65_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
     gene            complement(1288..1806)
                     /locus_tag="VNR65_01785"
     CDS             complement(1288..1806)
                     /locus_tag="VNR65_01785"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HWJ17434.1"
                     /translation="MAPAEKSAPATSKDAPASADKKAAQSDKLAPKAGEMKNDTSAPK
                     NAGATETKNPASPAPDPKAGAAKSSTDTKAGDTKSSTADTKGATAAPPAEKQTQISSA
                     IKQEKVEEVTNVNFNISIGTAVPSTVRYYPLPPRIIEIYPEWRGYDFILVRGKYIILR
                     PRTHEIVYIIEG"
     gene            2112..2351
                     /locus_tag="VNR65_01790"
     CDS             2112..2351
                     /locus_tag="VNR65_01790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWJ17435.1"
                     /translation="MIEKRVFPRHRALKAGTIEFDGGAINCMVRNMSNIGAALDVNSP
                     VGIPEHFTLVFPADGLGMPCRVAWRKEKRIGVVFD"
     gene            complement(2490..2681)
                     /locus_tag="VNR65_01795"
     CDS             complement(2490..2681)
                     /locus_tag="VNR65_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17436.1"
                     /translation="MLEWAKGGGGVRFALIVHHTDAEREWAYDRNSSVGKLDKALDVG
                     GLDRCRYEERLEDDIPVWE"
     gene            2952..3482
                     /locus_tag="VNR65_01800"
     CDS             2952..3482
                     /locus_tag="VNR65_01800"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ17437.1"
                     /translation="MITKYFVVGAIATALMVGTALSQTPAPATRTNSDAVAVDQAYKG
                     QWRLYKMIGLDVYNENNEKLGDIAELLTDQNGKIQTAILGVGGFLGMGEHQVAISFEK
                     LKFVNEPVVSTTASNSPATTTPATNVDNNARTTIGSATTNVRPVRTADEQWYPDHAVI
                     SATKDQLKAMPEFKYN"
     gene            3573..3773
                     /locus_tag="VNR65_01805"
     CDS             3573..3773
                     /locus_tag="VNR65_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWJ17438.1"
                     /translation="MDWNRVEGNWKEFQGKVKEKWGKLTDDDLTAINGKRDQLEGRLQ
                     QRYGFAKDQARKDIDSWFSALK"
     gene            4512..4961
                     /locus_tag="VNR65_01810"
     CDS             4512..4961
                     /locus_tag="VNR65_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17439.1"
                     /translation="MTESNGDWAPQRLCGIMIEARAPPMSVRKMHEIWIEQYDAAQDI
                     KLRYGLKAAFDYLVAEKLLNFANVAAQHPEFARDLPRFVSQVRLMFTPQEIRTHIARV
                     ECEQHENNAAAMEDDDLIRESPATAAERARQFATIKELLTATELGTS"
     gene            4967..7159
                     /locus_tag="VNR65_01815"
     CDS             4967..7159
                     /locus_tag="VNR65_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HWJ17440.1"
                     /translation="MARHQPESSTQEVLAGLVERVTFHNSDNGFCVLRTKARGHRDLV
                     TVVGHAAIISTGEWITASGEWVNDHTHGQQFKARFLRTSAPTSIDGIEKYLGSGMIRG
                     IGPVYAKKMVQAFGEKVFDIIEAEPDRLREVTGIGLVRAKRITDAWAEQKIIREIMVF
                     LHSNGVGTARAVRIFKTYGVDAVQVMTENPYRLARDIRGIGFKTADTIAMKLGIEKNA
                     MIRVRAGISYALTEAMDDGHCGLPTEELVPEAVELLEISKELVQTALDLELSEGTVIA
                     ATVGETACVFLGGLYRAEQVIAERLLRIWSGKLPWPYIDADKALPWVEQKTGLSLAES
                     QVAAIRLALISKVLVITGGPGVGKTTIVNTILRILDAKGVNLLLCAPTGRAAKRMTEA
                     TGFEAKTIHRLLEVDPKAGGFKRNSDNPLDCDLLVIDETSMVDVMLMQALMKAAPDDA
                     ALLIVGDIDQLPSVGPGQILADVIASGAVPVVRLTEVFRQAVQSRIITSAHKINQGSI
                     PDLSKPEGDSDFFFVQADDPETAVPRIIELVKTRIPQRFGLDPIRDIQVLCPMNRGGV
                     GARSLNIELQAALNPAGERKVERFGWTFAPGDKIMQIENDYDKEVYNGDIGYVDDVDP
                     DAGELTASFDGRAVTYGFGELDTLVPAYAATIHKSQGSEYPAVVIPVLTQHYAMLQRN
                     LLYTGVTRGKRLVVLVGQKKAIAIAVRNVSGRRRWSKLNEWLAVRAGG"
     gene            7164..7427
                     /locus_tag="VNR65_01820"
     CDS             7164..7427
                     /locus_tag="VNR65_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17441.1"
                     /translation="MAGDLQSFRERAARAVAKVARGSTADNADHYARMADAVIAVVAK
                     PTNAMIDAAYEAVRFDEAWAVNSRRDFVKAVNAMVRTTLNKRS"
     gene            complement(7886..8209)
                     /locus_tag="VNR65_01825"
     CDS             complement(7886..8209)
                     /locus_tag="VNR65_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17442.1"
                     /translation="MGLPVGLTLGRIVDTEGHNFTARWLLALAAIYDGATRTEAAKLG
                     GTILSGHAAKQPRGAGLPNQHARQGSVSSPWLCVPNGRRVPSDEPPPRRWEPDAGAAP
                     DRDGP"
     gene            9807..10148
                     /locus_tag="VNR65_01830"
     CDS             9807..10148
                     /locus_tag="VNR65_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWJ17443.1"
                     /translation="MTINGFAIAVISFALVGPLGAARAETGDAAYYRGGRTASGEVSG
                     PNGFTAAHRTLPFGTKVSVTNIRNGRSVIVRINDRGPYGRGRIIDVSRAAARELGMIS
                     SGTVRVRVDRQ"
BASE COUNT         2269 a         3050 c         3086 g         2283 t
ORIGIN      
        1 tgccgccacg tcaaccaact agccggcttg cgctttggtg gaggctcggg atcgggagac
       61 ggctcggcgt cgtagaacga cagcgcagct tccatcagtt cgagctgatg caggcgccag
      121 cggcccatcg cccggcggac gtgcttcctg gtcgcactgg cgatggccct agcctcggtg
      181 tcggtgacgt ccatctcctt catcaggagg aagacgacgt cgagcacgtt gaagagttcg
      241 ccatggcagt cgagatacca gtcgagctgg tcaggatctg actggtcgat ccacttgatg
      301 gcgcggttca cctcgccggc aagctcgtgg ttcagctcgc tagccagctc gtccctgtcg
      361 cagctgacgg ccttccggtc ataatcttcc cagcacctgg cacgggtgat gggcggctgc
      421 tcctgaggct ccagggcggc ttctacgggc tcgattaagg ttggattttc gggctgggag
      481 gcggtaagat caggctgttg ttgtttgtca gtacatccat aaaagcctcc tccagcccgc
      541 cagggtcgcc cccctggcgg gttgtctcgt ctggtcatgc aggcggcctc ccagcagttg
      601 ggaaaccgcg cgtaacgtat tgatccgata acatcgcttt tctctccagt tgggagaaaa
      661 gccgttttcc atcaatgtcg ggcacctgac tcttaatcat cgggtcccag gttcgagccc
      721 tggtgcgccc accaaataat gaagcgtttt caataacttg ctggacggaa gaatttcttg
      781 ttcttcaagg agattcttcg ggtcacgata gggtcacaac tcaactcagt tcgcatacgc
      841 acgagccaga ttgagacccc actgatagtg ccgtcttccg aagtagaagc ggggttcaga
      901 tcagcctccg atttgcactg ccgcttcctc gcgttcgtaa cggcgaactc cttccgccaa
      961 aatctcgcaa ccatgggatc tgtcacgcga gtacgtcagg ggttaggttt tcggtacaca
     1021 caggggcgaa aaaaaagaat caacggccca ccacctcagc cgagcttgcc ggccgtacgc
     1081 gacgccatcg tcgaactcat ccttcaacca ccgcctcaac gatgtccgta ctgcaaaagg
     1141 cgaatcgtcg aaaagcaacg gcgtgaataa atctgccaaa gtagtgctag tctgatctag
     1201 gcgcaggcgg cgccatttcg atggtctgct tagaacgtaa aaagggcggc cttgagagcc
     1261 gcccttttta tatattttcg agcgacgtta gccctcgatt atgtaaacga tctcatgagt
     1321 ccttgggcga agaatgatgt acttaccgcg tacaaggatg aaatcatatc cgcgccactc
     1381 cgggtagatt tcaataattc gcggcggcag gggatagtag cgaacggtgc ttggaacggc
     1441 cgttccaatt gagatgttga agttaacgtt cgtcacttct tcaacctttt cctgcttgat
     1501 ggcagaagaa atctgggttt gcttctcagc cgggggcgca gccgtcgcgc ctttcgtgtc
     1561 agcagttgag gacttagtgt cgccagcctt cgtatcagtt gaagatttgg cggctccggc
     1621 tttcggatca ggggcgggag aggcgggatt tttggtctcc gtcgcgcctg cattcttggg
     1681 cgcgctggtg tcgtttttca tttcaccggc ttttggagca agtttatccg attgcgcagc
     1741 tttcttgtca gcactcgcgg gggcatcctt agaggtcgcg ggtgcagact tttcagccgg
     1801 agccatcttt tccggcggcg cagactgttg cgccggagcg ggtgccgctg cacctccggg
     1861 actcgttcct tgggcggcgg caaatccgtt gcttgcgatc agcaacgcgg cagaaaacag
     1921 aaacttattc ctcatggaaa tctccttttg gcccccacct ggaacaattt cagccgggtt
     1981 aagttccgtc tggtgcagca tctatcaaag ccgccgttgg actcggacac taacggcggc
     2041 ttttcttttg ggccgcctca gttggcggcc tcttgggtcg attctttgaa ttcttggagt
     2101 gttccgactt catgatcgag aagcgggttt ttccacggca tcgcgccttg aaagccggaa
     2161 cgattgagtt cgacggcggc gcgatcaatt gcatggttcg caacatgtct aacatcggcg
     2221 cggcgctcga cgtaaacagc cccgttggca tccccgaaca tttcacgttg gttttcccgg
     2281 ctgacgggct gggcatgccc tgccgtgtcg cctggcgcaa agaaaagcgg atcggcgtgg
     2341 tgttcgatta aggtcggccg agagatttaa cgcctgtgtt gcgtcgaccg gttgagccgg
     2401 cagccaacaa tccgatccgt aaatttgcgc cgacgctcga tcttcttttc agagagttgt
     2461 tcgaaaatgg aaagcgttcg ggttctcatt tactcccaaa cgggaatatc gtcttccagt
     2521 cgttcttcat atcgacaacg gtccagccct cctacgtcga gcgccttgtc gagtttaccg
     2581 acggaggagt tgcggtcgta agcccattcc cgttcagcgt cggtgtggtg cacaatgagg
     2641 gcgaagcgga cgccgcctcc tccctttgcc cattcgagca tttgcaggtc gccgtctctc
     2701 gcatctccac gaaacctcct tcaatcaaag tcgccaaatc tacatgactg acaagcgata
     2761 gcttcgtttg tctcttatga tcttcgcaat tatttgtttt gccgtggaac cattttacct
     2821 cgtagcagta tggaaaagga gctcacaggt ttgcctcaat acaaccactg ccatgggtgg
     2881 cacctgtttg tgagcctgga gctttcccgc cttttcagag ccctgttaag gcaactaaat
     2941 ggagtaatcc gatgattact aaatattttg tcgttggagc catcgcgacc gctttgatgg
     3001 ttggcacggc tctttctcag accccggcgc ctgctacccg gacaaacagt gacgctgtcg
     3061 ccgtcgatca ggcctacaaa ggtcagtggc ggctctataa gatgatcggt ctcgacgtct
     3121 ataatgaaaa caatgaaaag ctaggtgata tcgccgaatt gttgactgat cagaacggaa
     3181 agattcaaac ggcgattctc ggggttggtg gttttctggg aatgggagaa catcaggtcg
     3241 caataagctt cgagaagctc aagttcgtca atgagccggt tgtctcaacg acggcttcga
     3301 actcacccgc tactaccacg ccagcaacga acgttgataa caacgcgcgg accaccatcg
     3361 gctctgccac tacaaacgtg cgccctgtac gcaccgctga tgagcagtgg tatccggacc
     3421 atgcagtaat cagcgccacc aaggatcaac taaaggcaat gcctgagttc aagtacaatt
     3481 gaccgaggag aaataagaag ggcccgcggc aagttgctcg cgggcctttt cataattggc
     3541 ctgtttttag atcttaaaga tggagataca tcatggactg gaatcgagtc gaaggaaatt
     3601 ggaaagagtt ccaaggcaaa gtaaaggaaa agtggggcaa gctcactgac gatgatctca
     3661 ctgcgatcaa tggaaaacgt gaccagctcg aaggccggct gcaacagcga tacggttttg
     3721 cgaaagatca agcccgtaag gacattgaca gttggttctc tgccctcaag taggttgtga
     3781 aagttgaggc cgggggcttt caaccccggc catgacattt agcacctatg tgtaccgaat
     3841 atcccaaccg cctaggagat aacgtttgtg accgagacag accttctcgg tggttggcta
     3901 gggcgcagcg cggtgagtcc ctgtggtcaa ccggcgatgt aggcagccga accggtcgga
     3961 tctgctcgtc tgtgtcgttt actagttcca tatggcaacg cggtcgccga gccggcttac
     4021 gcggcccatg gccacgaaat gctggccgcg tcctggccgc aggtagtcga atgcgccggt
     4081 cgatggtgcc gatactcgga aaagtggcgc gcgggcgtct cttcggcttt ccgccagcac
     4141 acctgtgcct gtcgcaaatc aaatccgaac atattggtgg tgcagcccgc cgagaattgg
     4201 gcgacaacaa atgtgcccgg ctagttcgac ggcgcgcgag atcggcgcat ctttctccaa
     4261 agataaatgc gtccttatct cgttgtgata tttcatgtac gacagcagta catgacggag
     4321 gtgtcgctcg ccgaacacaa caacgtggtc aaggcactcc cgtcggatcg aaccgatcag
     4381 ccgttcggca tatccgtttt gccatgggga gcgcggcgat gtcgggcgag atgcgggaag
     4441 ccgaggaagt cgacgaacca agacgtctcg gtcgatgaga cactgtgcgc gctctgagct
     4501 ctgatgtctg gatgactgag tctaacggcg actgggcgcc gcagcgatta tgcggcatca
     4561 tgattgaagc gagggccccg cctatgagcg tacgaaaaat gcacgagatc tggattgagc
     4621 aatacgacgc tgcccaggac atcaaactgc gatatgggct caaagctgca tttgattatt
     4681 tggtcgcaga aaagttgctg aacttcgcca atgttgccgc ccagcatcct gaattcgcgc
     4741 gcgatctgcc tcgattcgta tctcaggtga ggcttatgtt tacgccacag gagatacgga
     4801 cacacatcgc gcgggtcgag tgtgagcagc atgaaaacaa cgccgcagcg atggaagacg
     4861 atgatctgat ccgcgaaagt ccggctacag ccgcagagcg ggctcggcag ttcgccacca
     4921 tcaaagaatt attgaccgcc acggagctcg gcacctcgtg acgggtatgg cgaggcatca
     4981 accggaatct tcgacccagg aagttctggc tggccttgtc gaacgtgtca ccttccataa
     5041 cagtgacaat gggttttgtg ttctgcggac aaaggctcgc ggccatcgcg atctggtgac
     5101 ggtcgtcggc cacgccgcca tcatctcgac cggggagtgg attacggctt cgggggaatg
     5161 ggtcaacgat cacacccatg gccaacagtt caaggcgcgt tttctgcgca cgtcggcccc
     5221 gacgtcgatt gacggcatcg agaaatacct cgggtccggc atgatccgcg gcatcggtcc
     5281 ggtctacgcc aaaaagatgg tccaggcgtt cggcgagaag gtcttcgata tcatcgaggc
     5341 cgagcccgac cggctgcgcg aggtgactgg cattggtctg gtgcgtgcca agcgcatcac
     5401 agacgcctgg gccgaacaga agatcatccg cgagatcatg gttttcctgc acagcaacgg
     5461 cgtcggcacg gcgcgggcgg tgcggatctt caaaacctat ggcgttgatg ccgttcaggt
     5521 catgaccgaa aacccatacc gcctggcgcg cgacattcga ggtattggat tcaagaccgc
     5581 agatacgatc gccatgaagc tcggcatcga aaaaaacgcc atgatcaggg tgcgcgccgg
     5641 catctcatat gcactgacag aagccatgga cgacggtcat tgtggcttgc cgactgagga
     5701 gcttgtgccg gaggccgtcg agttgctgga gatatcgaag gagttggttc agaccgcact
     5761 ggatctcgaa ctgtccgagg gaacggtaat cgcggcaacg gttggcgaaa cggcctgcgt
     5821 tttccttggt ggcctgtacc gggccgaaca ggtgattgcg gagcgacttc tacggatttg
     5881 gagtggcaag ctcccctggc cctacattga cgccgataag gcgttgccct gggtcgagca
     5941 gaagaccggc ttgtcgttgg cggaaagcca ggtcgccgca attcgcctgg ccctgatttc
     6001 caaggtttta gtcatcaccg gcgggcctgg cgtgggaaag acgaccatcg tcaacaccat
     6061 cctgcgtatt ctggatgcca agggcgtcaa tctgctgctt tgtgcaccga ctggccgcgc
     6121 cgccaagcga atgacagaag ccacggggtt cgaggccaaa accattcatc gtctgctcga
     6181 ggtcgacccc aaggccggcg gctttaaacg caacagcgac aacccgctcg actgtgatct
     6241 gctggtcatc gacgaaacct ccatggtcga cgtcatgttg atgcaggcgt tgatgaaggc
     6301 agcccctgac gatgccgcgc tgctgatcgt cggcgacatc gaccaactcc cctccgtcgg
     6361 cccaggccag atcctcgccg acgtcatagc ctccggtgcg gtgccggtgg ttcgactgac
     6421 cgaggtgttc cgccaggccg tccagagccg gatcatcacc agcgcgcaca agatcaacca
     6481 gggctccatt cccgatctct ccaagcccga aggcgatagc gattttttct tcgttcaggc
     6541 cgacgatcca gaaacagccg tgccgcggat catcgaactg gtgaaaacgc gcatccccca
     6601 gcgctttggg ctcgacccca ttcgagacat ccaggtcctg tgcccgatga accggggcgg
     6661 cgtcggcgcc cgctcgctca atatcgaact gcaagcggcg ctcaatcccg ccggcgagcg
     6721 aaaagtggag cgcttcggct ggacctttgc gcccggcgac aagatcatgc agatcgaaaa
     6781 cgactacgat aaagaggtct acaatggcga catcggctac gtcgacgatg tcgaccccga
     6841 cgctggtgag ttgaccgcca gtttcgacgg ccgcgccgtc acctatggat tcggcgagct
     6901 cgacaccctg gtgccggctt atgcagcaac aatccacaaa agccagggct cggagtatcc
     6961 cgccgtcgtc atccccgtct tgacgcagca ctacgccatg cttcaacgga acctgctcta
     7021 cactggagtg acacgcggca agcggttggt tgtgctggtc ggccagaaga aggccatcgc
     7081 catcgccgtg cgtaacgtgt ctgggcgacg gcggtggtca aagctaaatg aatggttggc
     7141 agttcgtgca ggggggtaaa aagatggctg gcgatttgca gtcctttcgt gaaagagcgg
     7201 cacgtgcagt tgccaaggtc gctcgagggt ctactgccga taacgcagac cactatgctc
     7261 gcatggccga tgccgtcatc gcggtggtcg caaagccaac caacgccatg attgacgcgg
     7321 cctatgaagc ggtccgtttt gatgaggcct gggctgtcaa ctcacggcga gactttgtca
     7381 aagcagtgaa cgccatggtt cgaaccacgt tgaacaagcg ctcctaaatc gacagattcg
     7441 atcgaggccc ctggagcatt gtcacggttt tcggaatcat tgggatccac gctctcgtgt
     7501 ggaatctata cggaggcttc gataggaaaa gcctattcat aggacttgca tgagcgcgtg
     7561 attgcagctg tgtgaaccga aaataaggag tctggagacc gacaatcggt tcacagaagc
     7621 ccgtcttgct atcggtagcg gagacaatcc ctcacctttg gccatcaacc tctctcgccg
     7681 ccgcagtggc ggcgtctcag gcccccgttt tccggtgagt gccagtttcg accacgttcg
     7741 ttcggctcga taccccggat ggggacgacg caagcgggtc ggcagggtcg gcgccgccag
     7801 cagcctccaa ttttcaaaaa caagcgcggg tggacgaata cgcacctcgc gtcgtgcagc
     7861 tgtcaggcag gtctctggcg gcgtctcacg gcccatcccg gtcaggggcc gcccccgcgt
     7921 ccggttccca ccttcgaggt ggcggctcat cgctcgggac gcgacgacca tttggtacgc
     7981 acaaccatgg tgaggacacg gatccttgac gagcgtgttg atttggcagt ccggcgccgc
     8041 gcggttgctt ggcggcgtgt ccggatagga tggtgccgcc gagcttcgct gcctcggtgc
     8101 gcgtcgctcc gtcgtagatc gccgcaagag ccagaagcca tcgggccgtg aaattgtgcc
     8161 cctcggtatc cacgatgcgg ccaagcgtca ggcctacggg taagcccatc gctctgggct
     8221 ttcccagaag tgcgtcgacg cgtccatcaa aagccgccga tagtgttgca gcggcaaacc
     8281 gaatctaatc gccaaaaatc agaccggcga gcttcttttg ctgtcggatt tctccaaaag
     8341 gttcgagaac ttgagcattt ctcacttcgg cacgcaactt cccagccatc atggtcggct
     8401 ttgcagatcg ttctcgccaa atgccaacga gaccaacatc taccaacgac gaccaacacg
     8461 taccaagagg acctgattgc aggccaacag aaggccaaag aattgtcttg tcacacccca
     8521 cgcactaaag ctgccgcggc agtcccgtac cgcaaaacgg tcaggctata ggcccgcgaa
     8581 gcaaggggag cgccgcggta atgctggtca cgaccgcgct gcgagcgtca aggccgcgcc
     8641 tccagcgcgc cgcttcgcgg tccaggagcc ttgaccgccg cacgccctcg tgaaatcggc
     8701 tcggcatgcg cgccgcgcat attcatgcgc catcatttgt ggcggctcgc gtgtattcag
     8761 agcagttcgc gtcaggaatg cgatcgacgc cgatcggtgg cggcgttcgt attgctccgg
     8821 cggccttgcc gagagacgac gctacgcaat tggtctgcgg agatcagtcg cccgtttgcg
     8881 cggcgaacgt cccgcgatct taagaggcct gcaatgctca tccacgtcag gccgtgccga
     8941 cgcaactcaa gaaaaagcgg caagtgacgc gtcaccgcgc gctcgaaagg aatgtgtccc
     9001 agcgacccct gcaaagttgc gacgaatgcc ttgagacgtt tctcgatgtc cttgtccatg
     9061 tcgcttatat gatgagggat tgccacacac gcaacaagtc gcatcaatcc gcaacgagat
     9121 gcttcgagag gcaacaacga gaagaccacg tcgggcaata cattcgattt caaaatgctt
     9181 cgaagtgctt ctgtggtgcc cccaggtagt ggcaccattc accgggccag caggcccaca
     9241 tatcgaccac acggggacac acaggaacct ctcaggtgtc agccaacacc agtcagggcc
     9301 atcgtaaagc aaccaccgtt gccgcctgat ctcttcaggc ctcctgcaaa cccctgcaaa
     9361 acgcttcaat ctgcttcgat ttgcttcatc ttgctctgca gaggtttgac gcattttgca
     9421 acgaaatgca gcgaattgtt tcattttgtt tcgctcacac ctgtgcatcc ggccccggtg
     9481 atgcaggctg tggactgtcg gggagcgcgg agacgtcggc cgatcgcgaa tgcccattga
     9541 tcgaagtctt cggatgaaaa cgtgcccata ggcgccatcg cgatcacgga tcagatagcg
     9601 gggaatctgc tcccatccgc aggtctcggt gatctggttt gcgatccatt ccgcggtagg
     9661 ggatgtgccg tcacgccaaa ccaaatgatc tgccgtcgac catgtcccat gatcagtagt
     9721 ccgtatagga ggcgaattcg gaaaacatag ggatcggctg agacgccagc cttacagctc
     9781 ggtcggttcc gtgatattga tgcactatga ccatcaatgg tttcgccatc gctgttattt
     9841 cgtttgcctt ggttggaccg ctcggcgctg cccgggccga aacgggggat gccgcgtact
     9901 atcgtggtgg ccgcaccgcc agcggcgaag tgtccggccc aaatgggttt accgctgcgc
     9961 atcgcacgct tcccttcgga acgaaggtga gcgtcaccaa cattcgcaac ggtcggtccg
    10021 tgatcgtgcg catcaatgac cgcggtccct acggccgcgg acggatcatc gatgttagcc
    10081 gtgcagcggc gcgagaactc ggaatgatca gcagcggaac tgtcagggta cgcgttgatc
    10141 gacagtagct gacaaccaag tcgcctcttg aaacggtccg tcgctgcaga aattggggcg
    10201 tatgaccggg ttagcgcggc gccggccatt cggccggggc gcggcaaaga gtcggcggaa
    10261 aaattatggg tgaagtgagg cggcgatcgc gcctgccggt tcacgccaga ggtcggcctg
    10321 agttattcaa tcagatggaa atgtcgtcgc ttctcacgct gaacgcaatt gatcgcgcga
    10381 tcggcggttt tcttgtgggc tctcccgtac aatgtcaccc gaaccgaaaa gccagcgcta
    10441 ttatctcgaa taatttggat tccggcgacg gactcacacc ctggccactg tctaacggca
    10501 tcgatgcact cttttcgcag agccccgtag ctcattccga ctgtttcaga ttttgccatg
    10561 ctgcccataa tggccaggtt aacccaggct cgcgcgatca cgtcgtatcc gcagcatcat
    10621 cgggtcattt caccccggcg ggttttgacc tagatcaaag acggcgcccc cgattcgcgt
    10681 cggggggt
//
LOCUS       DATMGX010000109         4367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_16351, whole genome shotgun sequence.
ACCESSION   DATMGX010000109 DATMGX010000000
VERSION     DATMGX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16351"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2555)
                     /locus_tag="VNR65_01835"
     CDS             complement(<1..2555)
                     /locus_tag="VNR65_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HWJ17444.1"
                     /translation="MKRVRLDSPHGRSATEVLAALNVSFASGLSDHEAEQLLSVYGAN
                     AIGSRQKVGVISVLVHQLKSLVVALLAVAAAVAFYFREWEEGGAIVGVLILNTAIGFV
                     TEIKAVRSIEAIRALGSRSARVLRGGQTRAIPAERLVPGDIVLLDAGDVISADLRIVD
                     TADLDADESTLTGESMRVRKSVVPVKADARVADRTSMLFKGTSITRGSGTGVVVATGM
                     DTELGHISKLVAEAEAEASPLEKKLALLSGQLVWLTLALTALIGGIGLLQGKDAFLMV
                     EAAIALAVAAIPEGLPIVATLALARGMWRMARKNALVERLSAVETLGATTVILTDKTG
                     TLTENRMTVRRIWLSSGELSVGESSFELADRSAPVRPDTINELQCLLKIAVLCNNATL
                     GNVPAEDSGDPMELALLRAGRLAGLDRPALLSESAEIREHAFDTVRKMMATVHRRDDG
                     CLFAIKGAPEAVLASAKLDETSRDAWHARVAALGANGLRVLAFAKRTQPSRDDPAFEA
                     LTFLGLVGLEDPPRADVPDAIAACHAAGIRVIMITGDHSVTARSIARLVGLGGEAPRI
                     VEGHELVSLDGKVSEQFLQAQIFARVSPTEKLDLVRSYQASGEIVAVTGDGVNDAPAL
                     RQADIGVAMGLRGTDVAREAAAMILLDDAFPTIVEAIRNGRVIFGNIRRFATYLLSCN
                     LGEVSIVGIAMVSGLPLPLLPLQILFLNLVTDVFPAFALAMGEGESDILKRPPRDPKE
                     PILGRAQWLAIVLYGSTLTAATFGALALARLGLGLDEGVTVTITFLTLAFGQLWHVFN
                     MRHPSSRLILNEISRNPWIWAAIALCTGILLTAAYVPSLSFMLHLVAPDIRMWG"
     gene            complement(2973..4040)
                     /locus_tag="VNR65_01840"
     CDS             complement(2973..4040)
                     /locus_tag="VNR65_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWJ17445.1"
                     /translation="MRTIEDNAFLLLTVAVSLAFAWILLPFYGAVLWGTVTAILFSPL
                     HRRLLKSMGRRRNLAALSTVMIIIAIVILPMTLIAASLTQEALGFYGKVQSGELDLVR
                     FFQQVQDVLPVWAINLLDRFGLASLGAVQQKLSTTLMTGSQYIAAQALNIGQSTFDFI
                     ANLFVMLYLLFFLLRDEDTLSKRIRNAIPLHAEQQRAFLLKFTVVIRATVKGDMLVAL
                     LQGTLGGLIFWFLGISAPLLWAVVMAVFSLLPAIGAGLIWIPVAVYLLVTGAIWQGMV
                     LIAFGALVIGLVDNFLRPILVGKDTKMPDYVVLISTLGGIATFGLNGFVIGPVIAAMF
                     ISAWDIFSASREIAENDGTIR"
BASE COUNT         1016 a         1268 c         1231 g          852 t
ORIGIN      
        1 ccccacatcc tgatgtctgg tgccaccaaa tgaagcatga aagagaggct tgggacataa
       61 gccgcggtca gtagaatgcc ggtgcaaagc gcaatcgccg cccatatcca cggattccgg
      121 ctgatttcat tgaggatcag tcgcgacgat ggatggcgca tgttgaaaac atgccagagc
      181 tggccaaaag ccagggtcaa gaaagtaatt gtcaccgtca ctccctcatc cagcccaagc
      241 ccgagacgag ccaaggcgag cgctccgaac gttgcggcgg tcagcgtgga gccgtacagc
      301 acaatggcga gccactgggc gcggcccaga atcggctcct ttggatcgcg cggcggacgc
      361 ttgagaatgt cgctctcgcc ctcgcccatg gccaaggcaa aggccggaaa gacgtccgta
      421 acaaggttca ggaacaggat ctgcagagga agaagcggca atggcagccc ggagaccatt
      481 gcaatgccga cgatcgagac ctcaccgaga ttgcaggata gaaggtacgt cgcaaagcgg
      541 cggatattgc cgaaaataac gcgcccattc cggatcgcct caacaattgt cgggaaggcg
      601 tcatccagca ggatcatcgc agccgcttcg cgtgctacat cggtgcccct gagccccatg
      661 gcgacgccga tatctgcttg ccgcagcgcc ggtgcgtcat tgaccccgtc tccggtcacc
      721 gcaacaatct cgccagaggc ttgataggaa cgtacgaggt caagcttctc ggtggggctt
      781 acgcgagcga atatctgggc ctgcaggaat tgctcgctga ccttgccgtc gaggctgacc
      841 agttcatgac cttcgacgat gcgcggcgcc tcgccaccca atccaacgag tctggcaatg
      901 ctacgcgcgg tgactgaatg gtcccccgtg atcatgatca cccggatgcc cgctgcatgg
      961 catgcggcaa tcgcatcagg tacgtcggcc ctcggcggat cctcaagccc aacaagaccc
     1021 agaaaggtca aagcctcgaa ggccggatca tcgcggctcg gctgggtccg cttggcgaag
     1081 gccagcacgc ggaggccatt ggcgccaagg gctgccaccc gcgcatgcca cgcgtcacgc
     1141 gatgtctcgt cgagttttgc gctggcgagg acagcttccg gcgcaccttt tatcgcaaag
     1201 agacagccgt cgtcacgccg atgcactgtt gccatcattt ttcgaacggt atcgaaggca
     1261 tgctccctaa tttccgcgct ttcgcttagc agcgcggggc gatcgagtcc ggcaagccga
     1321 cctgcgcgca acagggcaag ctccatggga tcaccgctgt cttcggcggg aacattcccc
     1381 aacgtcgcgt tattgcaaag caccgcaatc ttgagcaggc attggagctc attgattgtg
     1441 tccggccgaa cgggcgcact tcgatcagcg agctcaaagc tgctttcacc tacgctcagt
     1501 tcgcccgagg aaagccagat cctccggacc gtcatgcggt tttccgtcag cgttccggtc
     1561 ttgtcggtga ggatgactgt cgttgcacca agcgtctcga ctgccgacag gcgctcgacc
     1621 aaggcattct tgcgcgccat ccgccacatc ccacgcgcga gcgcgagcgt agcaacgatc
     1681 ggtaaacctt ccggaatagc cgcgacggcg agcgcgatgg cggcttcgac catcaggaag
     1741 gcgtccttgc cttgcagtag cccgatcccg ccgataagcg cggtcagggc gagcgtaagc
     1801 cagacaagtt gtccggacag caatgcgagc ttcttctcaa ggggcgacgc ttcggcctct
     1861 gcctcagcga ccagcttcga gatgtgaccc agttcggtgt ccatgccggt cgcgacgacg
     1921 acgccggtgc cgctgccgcg cgtgatactc gtccctttga agagcatcga cgtgcgatcg
     1981 gcaacgcgag cgtcggcctt gaccggaacg accgatttcc tgacccgcat cgactccccg
     2041 gtcagcgtcg attcatccgc gtcaaggtcg gcggtatcga cgatccgcag gtccgccgaa
     2101 ataacgtcgc ccgcatcgag caagacaatg tctccaggaa ccagtcgctc tgccggaatg
     2161 gcgcgcgtct gcccgcctct gagcaccctt gccgaacggc ttcccagcgc tctgatggcc
     2221 tcgatcgatc gaacggcctt gatttctgtt acgaagccga tcgcggtatt gaggatcagg
     2281 acgcccacga ttgcgccgcc ttcctcccat tcccgaaagt agaacgcgac cgcagcagca
     2341 acggcgagca gtgcaaccac aaggctcttt aattgatgaa caaggacgga aatcaccccg
     2401 accttttgcc gcgagccaat cgcgtttgct ccgtaaaccg agagcagttg ctctgcctca
     2461 tggtcagaga gaccggaggc gaaagacaca ttcaacgcag cgagtacctc ggtggcgctg
     2521 cggccatgcg gagaatcgag ccgaacgcgc ttcatttcgc gagtatcctt agagcttgat
     2581 attaagcaac aaaaaacctg ctactctaaa ccgtgttcac tgtcatatgt tgagtcagcg
     2641 ccacgaatct cagccggagg aggaattatg atgccgtccc tcctaagatt ttatttgggt
     2701 tgaaaatgcc tcaaactaag tcggcaatga cagaacccgc atccggcatg ttccgaaaga
     2761 ttcgaatgga tgctgtcctc ctgcggtgag gagactgttc gcagaatgtt ggatatgaaa
     2821 aagcagaagc gcttgctgca tttccataaa gccgaacttt gccgaaaata ttctctatcg
     2881 gtcgaacgac ggaaaacacc tgcttctgct gagtttttcg cattttgacc catcagcgac
     2941 gtttgtcaga gtttgttaac tggtggtcga ccttaacgaa tggtgccgtc gttctcagcg
     3001 atctccctcg atgctgagaa aatatcccag gcagagatga acatggccgc gatcaccgga
     3061 ccgatgacaa atccgttgag accgaaggtt gcgataccgc ccaacgtgga gataagaacg
     3121 acgtagtcgg gcatctttgt gtctttgccc accaggatag gacgcaagaa gttatctacg
     3181 agtccgatga cgagcgcacc gaacgctatc aaaaccatgc cttgccagat agccccggtc
     3241 acaagaagat agacagcaac cggaatccag atgaggcctg cgcctatcgc gggtaacaat
     3301 gaaaagacgg ccatcacaac agcccataac aacggggcgc taatcccgag gaaccagaat
     3361 atcaggcctc cgagcgttcc ttgcagcaag gcgacgagca tgtcgccctt gaccgtggcg
     3421 cggatgacga ccgtgaattt gaggaggaag gccctctgtt gttcggcgtg taacggaata
     3481 gcatttctaa tgcgcttgga cagcgtgtcc tcgtcgcgga ggaggaagaa caagaggtac
     3541 agcataacga acaggttcgc gatgaagtcg aacgtgctct ggccaatatt aagtgcctgc
     3601 gcggcgatgt attggctgcc ggtcataagg gtggtggaaa gtttctgctg caccgcgccg
     3661 aggcttgcaa ggccgaagcg atccagcaga ttgattgccc atacaggcaa gacgtcttgg
     3721 acctgctgaa aaaatcggac gagatccagc tcgcccgact ggacttttcc ataaaagcct
     3781 aatgcttcct gcgtcaatga tgcggcgatc agcgtcattg gcagaatcac gatcgctatg
     3841 atgatcatga cggtagataa ggcagcaagg ttgcgccgcc gccccattga tttcaacagc
     3901 cgccggtgca acggggaaaa taatattgct gtgacggttc cccacagaac cgcgccataa
     3961 aatggcaaca agatccaagc gaaggctagt gataccgcaa cggtcagcaa caaaaaggca
     4021 ttgtcttcaa tggtgcgcat actgacattc catcaggcaa gctggagaag tccaagggca
     4081 atttaatgag ttcacgccat agatggaatc aattcgatgc agtaacgtcg ctcccactag
     4141 ttcaataaac tcctcagcta attatcgcac caaatcgcgc taaccggagc tgataggcgc
     4201 tatcgccttg ggcggctagc cggccaaatc gtgcaaggga caggctttgg gttggttggc
     4261 gacatttgga caatcgtcgg tgccttcata gccagttggc tgttcccccg gctcggtctt
     4321 catcttggtg ctgggacggc ggacctgcgt gttaggcatg cggaacc
//
LOCUS       DATMGX010000110         2574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_252636, whole genome shotgun sequence.
ACCESSION   DATMGX010000110 DATMGX010000000
VERSION     DATMGX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252636"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VNR65_01845"
     CDS             complement(<1..287)
                     /locus_tag="VNR65_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWJ17446.1"
                     /translation="MILSFGDYEIDIERRELRRAEIAVHVEPQVFDLLVYLVQNRDRV
                     VSKDDLIASVWGGRVVSDSTLTSRINAARTAVGDSGENQKLIRTIARKGLR"
     gene            461..688
                     /locus_tag="VNR65_01850"
     CDS             461..688
                     /locus_tag="VNR65_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HWJ17447.1"
                     /translation="MPLIEVHLIEKVFSPEQKRQIIHKLTDAMVSIEGENMRGVTWVK
                     ISEVTSGEWGIGGQPLTTEAVKDLAAGRHAA"
     gene            1642..>2574
                     /locus_tag="VNR65_01855"
     CDS             1642..>2574
                     /locus_tag="VNR65_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HWJ17448.1"
                     /translation="MKRVVTAALAVLGLVVFAGPALAQSYPNRTVTIIVPYSAGGPVD
                     QLARVIANALQAKLNQNFVVENVTGGSTITGTNKVVHSAPDGYTLLVHNLQISANATL
                     FKNLPYDTEKDLAPVMFIYRNPLVLVGRTTLSANSLEELIALMKKQRMKAAIPGFGST
                     GHLATALLAQEAKVPIDIIPYRGGAPMLTDVMGDHVDISFGTPQQVGPFVNAGKMKAF
                     GVTQDNKMPEFPQADSFVKMFGAKFGIQFWEAMFAPAGTPDAVIKTLNAALEEAVVTN
                     PAILKQWATEGVSAYPKDPMMTESGRSSCPRDRYC"
BASE COUNT          553 a          724 c          775 g          522 t
ORIGIN      
        1 aagcggaggc ccttgcgcgc gatggtgcgt atcaacttct ggttctcgcc gctgtcgccg
       61 actgccgttc gcgcggcatt gatgcggcta gtcaaggtcg aatcagacac gacccggccg
      121 ccccagactg aagcgatgag atcgtccttg ctgacgacgc ggtcgcggtt ctgcaccagg
      181 taaaccagca ggtcgaagac ctgcggctcg acgtgtaccg cgatttcggc gcgcctgagt
      241 tcgcgccgtt caatatcgat ctcgtagtca ccaaagctga ggatcattcg cgggccaagg
      301 gcatctggga cgtgccagaa agaaacaaag ggcgtctcgg gagcatctaa agagcacctg
      361 gagagtaaat catagcattc taaaagtctt gattggccga aacgtgcatc ctatcgccag
      421 tgccctagta cgggcgaggc cacaatagag gaagcagacc atgccattga tagaagtcca
      481 tctgatcgag aaagtattca gccctgagca aaagaggcag atcatccaca aattgaccga
      541 tgccatggtc tcgatcgaag gcgagaacat gcgcggcgtg acctgggtga agatttcgga
      601 agtgacgagc ggcgagtggg gcatcggcgg ccaaccgctg actaccgaag ccgtcaagga
      661 cctcgccgcc ggccgacacg cggcgtgacg tgtagtttgt tgggccgaca tcgccagcgg
      721 ggttatcctg ctggcgatgc ttgctcatgg caagattacc gctttgcttg ttgccaaggc
      781 ggcctgaacg gacggcaacc gaggttgtcc gcctcgctag ttttgttgca gtgcatgagt
      841 ccgtttatgg cacttcacgt cattcgtggc gctgcaacat ttcggtcgct aacggtgcaa
      901 agcggacatg gcgaaccggt ttagcgaacg aaccaaaagg tgaaatcctg agacggttgt
      961 tccgctcggg ctcgtgcgac catgtgcgtc acgagtataa agtgtgcgtc gcaaaaatgg
     1021 ctaaaggttc tcacgcgccg ttcgcctgtc agtgtgtcag cctgtggggc cgtgagcggg
     1081 tgccgaacgc tgaattggcg tgaccgaaac aacccgctat cgggcggaag gagttgcgaa
     1141 tttccacctt gaaaggtcgc tattggggca atagcggaca ttggccagaa atggcgctgg
     1201 ttttctcggt cgcgaatgat gatgagcgac attcgctgtg catcgcagca cttatcaaac
     1261 gccattccga cgcccacgat tgtgacaaaa tgtcttaaat tcaagggtgg ctaccgataa
     1321 ggcggcgacc tatcaaagtc gccattcagc aggcagctac tgccatcggt agggcaccca
     1381 tccacgcttt gtggtccgtg actacccaat gcgccctaat cggggccgtc gcctcgcagt
     1441 cgtaccgacg acttttgcac gatgcgcggc tcgcgccggg ttgcatctgt ttgtccacgc
     1501 tgtggacgcc gcgttgtcca cgttatggac tgcacgattg ccgccgcgac gcgtgcgggg
     1561 cggccaatct gcgcgaattc ttgtgagctg tcctccggaa ggtataggct ttcaaaaaac
     1621 agcccataga gaggatttcc aatgaagcgt gtcgtcactg ccgcgctggc ggtacttggc
     1681 cttgtcgttt tcgccggccc ggcgttggcg cagtcctatc ccaaccgcac cgtcaccata
     1741 atcgtgccgt actcggccgg cggcccggtc gatcagcttg cccgcgtgat cgcaaatgcg
     1801 ttgcaggcga agctcaatca gaatttcgtg gtcgagaacg tgaccggcgg cagcacgatc
     1861 accggcacta acaaagtcgt tcactcggcg cctgacggct acacgctgct cgtccacaat
     1921 ctgcagatct cggccaatgc cacgctgttc aagaaccttc cgtacgatac ggaaaaggac
     1981 ctggcgccgg tcatgttcat ttatcgcaat ccgctggtgc tggttggccg cacgacgctg
     2041 agtgcgaatt cgctcgagga attgatcgcg ctgatgaaga agcagcggat gaaagcggcg
     2101 attccggggt tcggctccac cggccatctc gccaccgcgc tattggccca ggaagccaag
     2161 gttccgatcg acatcattcc ctaccgcggc ggcgcgccga tgctgaccga cgtgatgggc
     2221 gaccatgtcg atatttcatt cggcacgccg cagcaggtgg gtcccttcgt caatgctggg
     2281 aagatgaagg cgtttggcgt gacgcaagac aataaaatgc cggaattccc gcaagccgac
     2341 agtttcgtca agatgttcgg agccaaattc ggcatccaat tctgggaggc gatgttcgcg
     2401 ccggccggga cgccggacgc cgtcataaag acgctgaatg cggcgctgga ggaggccgtg
     2461 gtcaccaatc ccgcgatcct caagcaatgg gcaacggaag gcgtgtcggc ctatccgaaa
     2521 gacccgatga tgacagaaag tggaagaagt tcttgcccta gggatcgcta ctgt
//
LOCUS       DATMGX010000111         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_16292, whole genome shotgun sequence.
ACCESSION   DATMGX010000111 DATMGX010000000
VERSION     DATMGX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16292"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VNR65_01860"
     CDS             <1..1170
                     /locus_tag="VNR65_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HWJ17449.1"
                     /translation="ATPIPRTLHMSLLGLRDMSVIETPPKDRMAIQTVVAAWDEKLLR
                     SAIEKELERGGQVYFVHNRVESIYEIADKLREMAPNARILIGHGQMPESELERVMLAF
                     MRHEADILVATTIIENGLDIPLCNTIIINRADRHGLSELYQLRGRVGRSDRRAYAYLL
                     IPPDRELSDLARRRLAALKEFSDLGAGFKIAALDLELRGAGNLLGGQQSGHIEAVGFE
                     LYTTMLERTVRELKGEVQEETAETQLNLGLNIRIPSDYIPEENQRLRMYKRVAGVETE
                     AQLEDVAGELGDRYGTPPPPVRSLLEYATLKLLSQRIGVAQIERRREAVSIRFTETAS
                     VDPERLARFVAGEPGSQFTPAGVLKFTLKEKQPEQVLARLKSLLEQLAGEQQAVA"
     gene            1224..>2012
                     /gene="galU"
                     /locus_tag="VNR65_01865"
     CDS             1224..>2012
                     /gene="galU"
                     /locus_tag="VNR65_01865"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HWJ17450.1"
                     /translation="MFPAAGLGTRFLPATKAQPKEMMPLVDKPIIQYGVEEALASGCD
                     QIIIVTGRGKSAIEDHFDVSYELEKMLEERGKTDLLAIVRQISEMIHIAYVRQKEAMG
                     LGHAVLMARELVGDEPFAVILADDIIDAEVPCLKQMIEVYNETGCSVLATQVIEGKAI
                     SSYGVLDAKPVPGKWNGRLFEVQNLVEKPKPEDAPSNLAIIGRYVLTPAIFEMLDSTP
                     LGAGGELQLTDGLRQLLKKEKIYGYTFEGKRHDTGDKLGFLKATV"
BASE COUNT          434 a          561 c          654 g          363 t
ORIGIN      
        1 gcaacgccca ttccgcgcac cttgcacatg tcgctgctcg ggctgcgcga catgagcgtg
       61 atcgagacgc cacccaagga ccgcatggca attcagacgg tggtggcagc gtgggatgag
      121 aagctgctcc gctcggcgat cgagaaggag ttggagcgcg gcgggcaagt gtactttgtg
      181 cacaatcgcg tggaaagcat ttacgagatc gcggacaagc tccgcgagat ggcgcccaac
      241 gcgcgcatcc tgatcggcca cggccagatg ccggagagcg aactggagcg cgtcatgctc
      301 gccttcatgc ggcacgaagc cgacatcctg gttgcgacca cgatcatcga gaacggactc
      361 gacattccgc tgtgcaacac catcatcatc aaccgcgccg atcggcatgg gttgtcggag
      421 ttgtatcagt tgcgcggacg cgttggccgc tcggaccgcc gggcctacgc ttacctgctc
      481 attccgcccg accgcgagct gagcgacttg gcgagaagga ggctggcggc gctcaaggag
      541 ttcagcgatc tgggcgcggg attcaagatt gcggcgctcg atctggaatt gcgcggcgcc
      601 ggaaatctgc tgggcggcca acagagcggc cacattgaag ccgtcggttt cgagctttac
      661 accaccatgc tggagcgtac ggtgcgtgag ctaaagggcg aggtgcagga ggagacggcc
      721 gaaacccagt tgaacctggg gctgaacatc cgtattcctt ccgactacat cccagaagag
      781 aaccaacggc tgcgtatgta caagcgcgtc gccggcgtgg agacggaggc gcaactggag
      841 gatgtcgcag gcgagttggg cgatcgttac ggcacgccgc ctccgccggt tcgaagcctg
      901 ctggagtacg caactttgaa attgctgtcg cagcgaatcg gcgtcgcgca gatcgagcgc
      961 cgccgcgagg ccgtgagcat tcgctttacc gagactgcct cggtcgatcc cgaacgcttg
     1021 gcgcgttttg tggccggaga acccggaagc cagttcaccc cggcgggcgt gctgaagttc
     1081 acgctgaaag agaagcagcc ggaacaggtg ctggcgaggc tgaagtcgct gctcgaacaa
     1141 ttggcgggag agcagcaggc agtcgcatga taagttagct ttcatttcat acaagaggac
     1201 ccatgaagaa ggtttgcaag gttgtgtttc cggcggccgg actgggcacg cgcttcctgc
     1261 ccgcgactaa ggcgcaacct aaagagatga tgccgctggt ggacaagccc atcatccagt
     1321 acggcgtgga agaggcgctg gcttctggct gcgaccagat catcatcgtg actggccgcg
     1381 gcaagagcgc gatcgaagac cacttcgacg tcagttacga gctggaaaag atgctggagg
     1441 agcgcggcaa gaccgatctg ctggccatcg ttcgccagat ttcggagatg atccacatcg
     1501 cctacgtccg gcagaaagaa gccatgggcc tgggtcacgc ggtcttgatg gcacgcgagc
     1561 tggtcggaga tgaacccttc gccgtgatcc tggccgacga catcatcgac gctgaagttc
     1621 cgtgcctgaa gcagatgatc gaggtttaca acgagacggg ctgttccgtg ctggcgacgc
     1681 aggtcatcga aggcaaagcc atctcttcgt acggcgtgct cgatgccaag ccggttccgg
     1741 gtaaatggaa tggccggctc tttgaagttc aaaacctggt ggagaagcca aagccggaag
     1801 atgcaccctc gaacctggcg atcatcggac ggtacgttct cacgcccgca atttttgaaa
     1861 tgctggattc aactccgctg ggcgcgggcg gcgagttgca gctcaccgac ggcctgaggc
     1921 aactgctcaa gaaggaaaag atttatgggt ataccttcga aggtaagcga cacgataccg
     1981 gcgataagct gggcttctta aaggcgacgg tg
//
LOCUS       DATMGX010000112         3930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_275200, whole genome shotgun sequence.
ACCESSION   DATMGX010000112 DATMGX010000000
VERSION     DATMGX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275200"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1723
                     /locus_tag="VNR65_01870"
     CDS             155..1723
                     /locus_tag="VNR65_01870"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ17451.1"
                     /translation="MTHELSNPSFHPAGEQPPVEGKGPPQGVILDSFSGPVLVEWDTE
                     AALTPLGQLPFFIDFLKAAGLFDAFVADCPLRYTSPNAPKKRDVLGTAMLSMLSGHKR
                     YAHIAALRCDSVLPELLGMNKIVSEDAVRRAFAAIDEDDGAVWLRRHLDYCSAPLLAE
                     PWILDIDTTVKPLYGHQEGAVIGYNPKKPGRPSHSYHTYSMAGVRLVLDVDVVAGNEH
                     TSKHFAPGLWALLDRIPRDLWPSLLRGDCGFGNEPIMREAEQRGLAYLFKLRLTANVK
                     RMIERLSTQREWANTGQGWQAKESLLRLEGWSRQRRVIILRRRVKGDLAISSNETSGQ
                     QTLSFVDVGAGVDVYEYSVLATSLDEELVSFGQLYRDRGDSENIFDELKNQWGWGGFV
                     TQDLARCRLAARMIALFYDWWSIFVRLVEPDRHMEAITSRPLLLHAIATRVRHARQTT
                     ITVASSHARAIPAAKAFRAVAIFLRGLAKNAEQLTGLQRWRRILARAFQVFLQGRQLR
                     SPPRLCPADATTFG"
     gene            1904..2665
                     /locus_tag="VNR65_01875"
     CDS             1904..2665
                     /locus_tag="VNR65_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ17452.1"
                     /translation="MDDNLCVTHTEQIRIEQQLCQEAQRAALDRSGALSTQAIREAMA
                     ALRKDDPTIAFTEQQEASIYALGQGSKLSLLTGVAGSGKTTLLKPLVTAWKAGGRALA
                     GMSTAWRQADALKDASIEETWALQPLLRAIDAGEFQPTEKTVLIIDEISQIARRPMLK
                     LLELQAKTGMTIKMLGDREQCQSIEAGDTIALLRRVLPKFALPELLTAVRQKDPGDRK
                     IASLFREGKAAEAFQMKREDGTATLLEGDYDQVVG"
     gene            2726..3772
                     /locus_tag="VNR65_01880"
     CDS             2726..3772
                     /locus_tag="VNR65_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17453.1"
                     /translation="MTVTTLTNAEAADISQAIRRRLKERGKIGQDETIHKAVYYKGDK
                     PEFFDLPIASGDKLRLYRRTWAKIDGKGASIGNNGDIVDVVGKTAGGLLLRDARGRVG
                     EVEWRRLSDQETGRLLLGFGRAFTVDAAQGMSTRGEHANALAHGTGGSTAFKTYTGES
                     RATGKTHTLISKAAVLSDVRRSRALGDTTPITEDALWKRIGEDTSAKPYKALAIDLLA
                     AARRHSDQAVDVSLRAHQRIETAASAKTNVGRAVRATVEAGAAREALARQRISLEETL
                     QRCATMLKDAAEMIMDHLHGRRPAAQATDVSVPIPAATAAPEAPSPPSATGPALAPEA
                     ELPSPPRPSPSPGM"
BASE COUNT          801 a         1196 c         1165 g          768 t
ORIGIN      
        1 tcaggccgtt cgagtgtcac cttgaaaaag aaccggagcg cgacgatcgc gcaattgatg
       61 ctcggagcgc caacatggtt ccttgtcata tgtaatccta taaacggcag ttggctggtt
      121 tgtatttggc gagaatcacc gcattatcag gagcatgacg cacgaacttt ctaatccatc
      181 ctttcaccct gcgggtgagc aaccgccggt tgaaggcaaa ggcccgccgc aaggcgtgat
      241 cctcgacagt ttctccggcc cggtgcttgt cgaatgggat accgaggcgg cgctgacgcc
      301 gcttgggcaa ttgccgttct tcatcgattt tctgaaagcg gctggcctgt tcgacgcttt
      361 cgtggcggat tgcccgctgc gttacacgag cccgaacgcg ccgaaaaaac gcgatgtgtt
      421 gggaacggcg atgctgtcga tgttgtctgg gcacaagcgt tatgctcaca tagcggcgtt
      481 acgttgcgat agcgttcttc cagagcttct cggcatgaac aagatcgtta gcgaggacgc
      541 cgttcggcgc gccttcgcgg cgatcgacga agatgacggc gcggtgtggc tgcgacggca
      601 tctcgattat tgcagtgcgc cgcttcttgc cgagccgtgg atactcgaca tcgatacgac
      661 tgtgaagcca ctctacggcc atcaggaagg cgcggtgatt ggctacaacc ccaagaagcc
      721 cgggcggccg agccattcct atcataccta ctcgatggct ggcgtacggc tggtcctcga
      781 tgtcgatgtt gtcgccggca acgagcacac atccaaacat ttcgcgcccg gcctgtgggc
      841 gttgctcgat cgcatcccgc gtgatctgtg gccgtctctg ttgcgtggcg actgcgggtt
      901 cggcaacgag ccgatcatgc gcgaagcgga acagcgcggc ttggcctatc tcttcaagct
      961 gcgattgacc gccaatgtca agcgcatgat cgaacgtctg tccacgcaaa gggagtgggc
     1021 gaacaccggg caaggctggc aggccaagga gagcctatta cggctcgagg ggtggagcag
     1081 gcagcgccgc gtgatcatat tgcggcggcg ggtgaagggc gacttggcga tatcttcgaa
     1141 tgagacgtca ggccagcaaa cattgtcttt cgtcgatgtg ggcgcgggcg tcgacgtcta
     1201 cgaatattcc gttctcgcca catcgctcga cgaggagctt gtgagtttcg gccagctgta
     1261 tcgcgatcgc ggcgatagcg agaatatttt tgatgaactg aaaaaccaat ggggctgggg
     1321 cggcttcgtc acgcaggacc ttgctcgttg ccgactggcc gcgcgcatga tcgcgttgtt
     1381 ttacgactgg tggagcatct tcgttcgtct ggtcgagccg gaccggcata tggaggccat
     1441 caccagccgg ccactgctct tgcatgccat cgccacgcgg gtgcggcatg cgagacaaac
     1501 aacgatcacc gtagcgagtt cacacgcgag ggcgattccg gccgcgaagg cgtttcgcgc
     1561 tgtcgccatt tttttgcgcg ggctggcaaa aaatgcggag cagttgacgg gcctgcaacg
     1621 ctggcggcga atcctcgcga gagccttcca agttttcctt cagggccgtc agttgcgctc
     1681 gccgccacgc ctctgcccgg ctgacgcaac aacttttggt tgagaaatca cagaaatcag
     1741 ttgcgcgtcc aactccaact gccggattta ggttcatgcc gtccgtggcc ttatcggcac
     1801 cggcatcggc ggcggccgcg atgatgtcga tcgcgtcgtc gccctcatcg aagagcgcgg
     1861 tctggaggtc aatggcgtcc atgtctctct cgtcaagggg acattggacg acaacctttg
     1921 tgtcactcat accgagcaaa tccggatcga gcagcagctc tgccaagaag cccagcgagc
     1981 ggcacttgac cgctccggcg ctctctccac acaagccatc cgtgaagcca tggccgctct
     2041 ccggaaagac gatcctacca tcgccttcac ggagcagcag gaggcctcaa tttacgcttt
     2101 gggccagggc agcaagctat ccctgctgac cggtgttgcc ggatccggca aaacaaccct
     2161 cctaaaaccc ctcgtcaccg cctggaaggc tggtggccgt gcgctcgccg ggatgtcgac
     2221 ggcctggcgg caggccgatg cgctgaagga cgcttctatc gaggagacct gggcgctgca
     2281 gcccttactg cgagccatcg atgcgggaga gtttcaaccc accgaaaaga ccgtgctcat
     2341 tatcgacgaa atcagccaga tcgcccgtcg ccccatgctg aaactgttgg agttgcaggc
     2401 gaagaccggc atgaccatca aaatgctcgg cgaccgcgag caatgccagt cgatcgaggc
     2461 cggcgatacg atcgcgctct tgcgccgcgt tctgccgaaa ttcgcattgc cggaactcct
     2521 gaccgccgtg cgccagaaag atcccggcga tcgcaagatc gcgtccctct tccgtgaagg
     2581 caaggcggcc gaggctttcc aaatgaagcg cgaagacgga accgcgacgc ttcttgaggg
     2641 cgattacgac caagtcgtcg ggtagattgc cgacttctat atcgagcgca acgatcttct
     2701 ccgcgccgtt gacgataaat tcagcatgac cgtaacgacc ttgaccaacg cggaagccgc
     2761 cgacatcagc caggccatcc gccggcggct taaagaacgt ggcaagattg gccaggatga
     2821 aacgatccac aaggccgttt actacaaagg cgacaagccg gaattcttcg atttgccgat
     2881 tgcaagcggc gacaaattgc gcctctaccg gcgtacctgg gccaagatcg acgggaaagg
     2941 agcatcgatc ggcaacaatg gcgacattgt cgatgtcgtc ggcaaaaccg ctggcggcct
     3001 acttctccgc gatgcacgcg gccgcgtcgg ggaggtcgaa tggcggcggc tctccgatca
     3061 ggagacagga aggctcctcc tcggtttcgg ccgcgccttc accgtcgacg ctgcccaagg
     3121 catgagtaca agaggcgagc atgccaacgc gctggcgcat ggaaccggcg gctctaccgc
     3181 cttcaaaacc tataccggtg aaagcagagc cactggcaaa acgcacacct tgatatccaa
     3241 agccgccgtt ttgtctgacg tcaggcgcag ccgggcgctt ggcgatacga cgcccataac
     3301 cgaggacgcc ctctggaagc gcatcggaga agacacgtcg gccaagccct ataaagcgct
     3361 ggcgatcgat ctcctggccg cggcacgccg tcacagcgat caggctgtcg acgtctcact
     3421 ccgcgcccat cagaggatcg agacggcagc aagcgccaag accaatgtgg gccgagcagt
     3481 ccgtgcgacc gttgaggccg gagccgcgcg cgaggctctt gcccgtcagc gcatctccct
     3541 cgaggagact ctccagcgtt gtgcaaccat gctcaaagac gccgctgaga tgatcatgga
     3601 tcacttgcat ggcagacgcc cggcggcgca agccactgat gtttcagttc ccattcctgc
     3661 cgccaccgca gcgccggaag ccccctcgcc tccctcagcg accggtcccg cgctcgctcc
     3721 cgaagcagag cttccctcgc cgccgcgccc gtcgccttct ccaggcatgt gactgcggcg
     3781 cctattcttc ctttcgataa atgggttatc cggatttcag cgtgtttgct gtatcgcaga
     3841 cggatcgtcg aacaaatttt gatcaatgtg ttttgtcgcc tgacaaaacg tagatttcaa
     3901 gttcgcgtgc ggactacgct cagccttaac
//
LOCUS       DATMGX010000113         2167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_444030, whole genome shotgun sequence.
ACCESSION   DATMGX010000113 DATMGX010000000
VERSION     DATMGX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444030"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VNR65_01885"
     CDS             <1..813
                     /locus_tag="VNR65_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17454.1"
                     /translation="HDVNRQARQWLAEVANVRLHHETRQRPIDRFRADALRPLPVIPY
                     DYRDTVEALVHKDLRLQFDGNRYCVPHRFVGRRLTVKADSSSVTIYHRFQEIVSYARS
                     WRRGQTFGAERFERELVEQRPAARRSQAQQRFLGFLDGLCAPDVAEGYLRGVADSDRV
                     LSRQLNELLELIRQYGPEAVAGAVAKAYAARAFGADYVANILRQQQAPRRPQPPLRLR
                     DPRLNELVTDPLSLLAYDAFILDSGKECDDAPRETGPTEPVHDEPPTGDDDL"
     gene            782..1486
                     /locus_tag="VNR65_01890"
     CDS             782..1486
                     /locus_tag="VNR65_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ17455.1"
                     /translation="MSRQLETTISEAATRNLSVTATLEWLADLELEARNHRAIERRFK
                     CSRLQAQPCIDAFHFHHHKTRLQNKNRILRLLDLEFLHKGTNLVFIGNPGVGKTFLSK
                     IVGWRICQANQRILFTTAMDMLNHLLASQVDHSLVRKLKTYTEPTALVVDELGYLALD
                     QQTSNLFYQVISTRHSQKRSTIITTNTAFSDWGNILFNTTIATAIADRLVENSEIFLL
                     GGDSLRKSKNPNPPAE"
     gene            1628..1915
                     /locus_tag="VNR65_01895"
     CDS             1628..1915
                     /locus_tag="VNR65_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17456.1"
                     /translation="MFNFSANHLFLLSRTEYRSCAVFLMQDRDTQRVYRLYDFTKAQL
                     IPPDMAHCIAGKVNSAGRLYLVIESVRPDTKHLQASNAPAHVDGAEGSLIE"
BASE COUNT          424 a          737 c          583 g          423 t
ORIGIN      
        1 cacgatgtga accgccaggc gcgacagtgg ctggccgagg tcgccaacgt gcgcctccat
       61 catgagaccc ggcagcgacc catcgatcgc ttccgtgccg atgccctacg cccgctgccg
      121 gtgatccctt acgattaccg cgacacggtg gaggccctgg tccacaaaga tctccgcttg
      181 cagttcgatg gcaaccgcta ctgcgtccct catcgctttg tcggccgtcg gctcaccgtg
      241 aaagccgatt ccagttccgt caccatctac catcgcttcc aggaaatcgt ctcctatgcg
      301 cgcagttggc ggcgcggcca gacttttggc gccgagcgct tcgagcggga actggtggaa
      361 caacgcccgg cggcccgccg ctcgcaggcg cagcaacgct tcctcgggtt tctggacggc
      421 ctctgcgccc ccgacgtagc cgagggctat ttgcgtggcg tggccgatag cgaccgtgtt
      481 ctgtcacgcc aactcaacga actgctggaa ctgatccggc agtatggacc ggaggcagtg
      541 gccggcgccg tcgccaaagc atatgccgct cgcgccttcg gcgccgacta cgtcgccaac
      601 atcctccgcc agcagcaagc gccgcggcgg ccgcagccac cgcttcgtct ccgcgacccg
      661 cggttgaacg aactcgtcac cgatccgctg tcgctgctgg cctacgatgc tttcattctt
      721 gattccggaa aggaatgtga tgacgctccc cgagaaactg gaccaactga gcctgtccac
      781 gatgagccgc caactggaga cgacgatctc tgaggccgcc accaggaacc tcagcgtgac
      841 cgccacgctg gaatggcttg ccgatctgga actggaggcc cgcaaccacc gcgccattga
      901 acgccgcttc aaatgctcgc gcttgcaggc gcagccctgc attgacgcct tccactttca
      961 ccatcacaag acccggctgc aaaacaagaa ccgcatcctg cgcttactcg acctggagtt
     1021 tctgcacaag ggcaccaatc ttgtgttcat cgggaatccc ggggtgggaa agaccttcct
     1081 atccaaaatc gttggctggc gcatctgcca ggccaatcag cgcatcttgt tcaccaccgc
     1141 catggacatg ctgaatcatc tgctcgcttc gcaggtggat cactcgctgg tccgcaagct
     1201 caagacctat acggagccca cagcgctcgt cgtcgatgag ctaggctacc tggccctcga
     1261 tcagcaaacc tcgaatctct tttatcaggt catctccacc cgtcacagcc aaaagcggtc
     1321 aacaattatc actacgaata ctgcgttctc cgactggggc aatatcctct tcaacaccac
     1381 catcgccacc gccatcgccg accggctcgt ggaaaactcc gagatcttcc tgctgggcgg
     1441 agacagcctt cgcaagtcca agaatccgaa tccgccggcc gagtagttgc gatcggctcc
     1501 gagcaagcgc cgcgctgtgc gataacccgt cacggaggag tccgccgggc taacccctca
     1561 acggcgctgc caatgggggc gggagactca actcccgcct cctgcttgat tcctcaaccg
     1621 ttgtccaatg ttcaacttca gcgccaatca tctgttcctg ctctcgcgga cggaataccg
     1681 ttcgtgcgcg gtgttcttga tgcaggaccg cgacacccag cgcgtttatc gtctgtacga
     1741 cttcaccaaa gcccagctca tcccgcctga catggcgcac tgcatagcgg gcaaggtcaa
     1801 cagcgccggt agactctacc tggtgatcga atcggtccgg ccggacacca agcacctgca
     1861 ggcttccaat gctcctgctc atgttgacgg cgcggaaggg tcgcttatcg agtaaacgcc
     1921 aatgctttgc gcaaccggcc aactgccgcg ctacgcctca aatcgctgcg cgggatcgcc
     1981 gtcgtgtgcc tcggcgccga cgccggcgct cgccacggcg tcaaaaccgg cgaaaacgct
     2041 gctcttttaa accggcgcta acagtgaacg ttcgagttgc catttgatga caattatatc
     2101 cggtgcttga gagtaccgac atgtaagggt tttcggttca tgccgccagg ctggttcctt
     2161 cggtcct
//
LOCUS       DATMGX010000114         2442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_249648, whole genome shotgun sequence.
ACCESSION   DATMGX010000114 DATMGX010000000
VERSION     DATMGX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249648"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VNR65_01900"
     CDS             <1..644
                     /locus_tag="VNR65_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ17457.1"
                     /translation="SAAFVEPALDYFGEHGGTVAFGKRLREIEFGSGHVRSLKFSDSE
                     ISVREGEAVVLAVPPWVAQALVPGLKAPTEFRAILNAHFSVAPPPQFPPILGLIHGTV
                     EWIFSFPGRMSITISGADRLIDRPREELAAELWRDVAAVTGLPAELPPWQIVKEKRAT
                     FAASPDQDVLRPEPSTGWRNLFLAGDWIQTGLPATIEGAIRSGERAARLAMRD"
     gene            658..>2442
                     /gene="shc"
                     /locus_tag="VNR65_01905"
     CDS             658..>2442
                     /gene="shc"
                     /locus_tag="VNR65_01905"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HWJ17458.1"
                     /translation="MSERAVREDDNYEAERFEASQSAEGEPAGNQLEDRIRAASLALR
                     RHQQEDGHWCFEFEADATIPAEYILLTHYLGEEADLKREAKIGTYLRRIQGKHGGWPL
                     FHEGAFDMSASVKAYFALKMIGDDINTPHMLRARQAILSRGGAEMSNVFTRILLALYG
                     VVTWRAAPVMPVEIMLLPKWFPFHMTKVSYWARTVMIPLYVLAALKPVARNKRGVTLD
                     ELFFTPPKTVGMRPRAEHQDRSWFWTFAAIDKVLRVVEPLFPVPTRRIAIEKAVAFVK
                     ERLNGEDGLGAIFPAMANSVMMYDALGVPESDPDRAIARHSVEKLLIETENEIYCQPC
                     VSPVWDTGLAAHSLLEAGGEAERESAAKGLEWLKPRQVLDVKGDWADQRPDVRPGGWA
                     FQYANPHYPDLDDTAVVVMALDRAQNVPGNREDHGTAISRGREWIAGLQSKNGGWASF
                     DADNTHMYLNNIPFSDHGALLDPPTVDVSARCVSMLAQLGETPETSPMLKRGIDYILS
                     DQEKDGSWFGRWGMNYIYGTWSALCALNAAGLSHSAPAMRRAVAWLLSIQNDDGGWGE
                     DGSSYKLGYKAYEKSPSTPSQTSWALLGL"
BASE COUNT          480 a          731 c          753 g          478 t
ORIGIN      
        1 tttcggccgc tttcgtcgag cctgcgctcg actatttcgg cgaacatggt gggacagttg
       61 cattcgggaa gcgccttcgc gagatcgagt ttggttccgg ccacgtccgc agtctcaagt
      121 ttagcgatag cgagatttca gtccgcgaag gcgaagccgt cgtccttgct gtgccaccct
      181 gggttgcgca agcgcttgta cccggcttaa aagccccaac cgagttccga gccatactga
      241 atgcgcattt cagtgttgcg cctccgccgc aatttccacc gatccttggc ctcattcacg
      301 gcacagtcga atggatcttt tcatttcctg gccgtatgtc tatcactatc agcggagctg
      361 atcgcttgat cgatcgcccc cgcgaggaac ttgcggctga gctgtggcgg gatgttgcgg
      421 ctgtaacggg gctaccggcg gagctacctc cttggcagat cgttaaggaa aagcgggcaa
      481 cattcgccgc gagtcccgat caggatgttt tgcgaccgga accaagtaca ggttggcgca
      541 acctttttct cgcgggcgac tggatccaga cgggcctccc cgcgaccatc gaaggggcaa
      601 ttcgctctgg cgagcgggct gcccggctgg caatgaggga ctagggtaac tatgactatg
      661 agcgaacgcg ctgtacgcga ggatgacaac tacgaggctg agcgtttcga ggctagtcag
      721 tcagccgaag gcgaacccgc gggcaaccag ctggaagatc gcatccgggc tgcatcacta
      781 gcgcttcgcc gccatcagca ggaggacgga cactggtgct tcgagttcga agccgatgcg
      841 acaatcccgg cggaatatat cctcctcaca cattacctgg gcgaggaggc ggacctcaag
      901 cgcgaggcaa aaatcggcac ctaccttcgc cgcattcagg gcaagcatgg cggctggccg
      961 ctctttcatg agggcgcgtt cgatatgagc gctagcgtga aagcctactt cgcgctgaaa
     1021 atgatcggcg acgacatcaa cactccgcac atgctgcgcg cccgccaagc gatcctttcg
     1081 cgcggcggcg cggagatgag caacgtcttt acgcgcatcc ttctcgccct ttacggcgtg
     1141 gttacatggc gcgcggcccc ggtgatgcct gtcgaaatca tgctgctgcc gaagtggttt
     1201 ccgttccaca tgacgaaggt gtcctactgg gcacgcaccg tcatgattcc gctttacgtg
     1261 ctcgcagcgt tgaaacccgt ggcgcggaac aagcgcggcg taaccctgga cgagttgttc
     1321 tttacgccgc cgaagactgt gggcatgcgg ccgagagcag agcatcagga ccgatcctgg
     1381 ttttggactt tcgcggccat cgacaaggtc ttgcgagtgg ttgagccgct gtttccggtg
     1441 cccacgcgcc gcatcgccat cgagaaggct gtcgccttcg tgaaggagcg gctgaacggg
     1501 gaagatgggc tcggcgccat cttcccggca atggccaaca gcgtgatgat gtacgacgcg
     1561 ttgggcgtcc ccgagagcga tccggaccgc gccatcgccc gccattcggt cgagaagctt
     1621 ctcatcgaaa ccgagaacga gatttactgc cagccttgcg tttcgccggt ctgggatacg
     1681 ggcctcgccg cccacagcct gctcgaagcc gggggtgagg ccgagcgtga aagcgcggcc
     1741 aaggggctcg aatggctgaa gccgcggcaa gtccttgacg tcaagggcga ttgggccgac
     1801 cagcgccctg atgtcagacc gggcggctgg gcgttccaat acgccaatcc gcattatccc
     1861 gatctcgacg acacggcagt ggtggtcatg gcgctggatc gtgcgcagaa tgttcccggc
     1921 aatcgcgagg accatggcac cgcgatcagc cgcggacgcg agtggattgc tggcctgcaa
     1981 agcaagaacg gcggatgggc ttccttcgac gccgacaaca cgcatatgta tctaaacaac
     2041 attccgttct cggaccatgg cgcgcttctc gatccgccaa cagtcgacgt aagtgcgcgc
     2101 tgcgtgtcaa tgctcgcgca gctgggagag acgcccgaaa caagccccat gctcaagcgc
     2161 ggcatcgatt atattctgag cgaccaggaa aaggatggaa gctggtttgg ccgctggggc
     2221 atgaactaca tctacggcac ttggtcggcg ctttgcgcgc tgaatgctgc cggcctcagc
     2281 cattccgcgc ccgccatgcg gcgcgccgtt gcttggcttc tttccatcca aaatgatgat
     2341 ggcggctggg gcgaagacgg ctcgagctac aagctcggct acaaagccta tgaaaagtct
     2401 ccaagtacgc cctcgcaaac atcatgggcg ctgctggggc tg
//
LOCUS       DATMGX010000115         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_126655, whole genome shotgun sequence.
ACCESSION   DATMGX010000115 DATMGX010000000
VERSION     DATMGX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126655"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VNR65_01910"
     CDS             <1..1005
                     /locus_tag="VNR65_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17459.1"
                     /translation="DGSKLSQPLSAQLIADGDEDQDDVAEALAAAPQGRRAQIVAERI
                     VERIIERVRLKTERDKLPNRRKGYTQKAVVGGHKVYLRTGEFEDGRLGEIFIDMHKEG
                     AAFRSLMNNFAIAISLGLQYGVPLDEYVEAFTFTRFEPAGYVQGNEAIKNATSILDYI
                     FRELAVSYLGRNDLAHVDPSEIGATVIGGGVADDRPGQKASAPVIYADRYVSRGLTRG
                     LHHKVVIVQDQPKTERISSAGSYETRGALAYALSTDDENVPLGSSNVNARDGSSAALS
                     ASHLTVSNGAKPGKAELLAEARAKGYEGEACGTCGNFTLLRNGTCMKCDTCGGTTGCS
                     "
     gene            1099..1743
                     /locus_tag="VNR65_01915"
     CDS             1099..1743
                     /locus_tag="VNR65_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17460.1"
                     /translation="MALWTLNPPQQEDIYTVVYFLRDTLSSALEDAWFFEKTALPKAD
                     VKGAGHAEIAARLGRFRDGCKTFQEREALMLMKLSRARIWAAELRKMAPEVQHDVDQF
                     LDATEACDEMQAEFVRDAHRMFNGGGLLSRFLARRKPEAEERQSHCAHDGSYRVGGRT
                     ELFELRAACEVFLAQINDGFFPSNHPENAALTKPLVAYLEAPQEPAPAVVPEVH"
BASE COUNT          487 a          671 c          665 g          467 t
ORIGIN      
        1 gacggttcaa agctctctca accgctctcc gcgcaactca tcgccgatgg cgacgaggat
       61 caggacgatg tggcggaggc tctggcagcg gctccgcagg gcaggcgcgc tcagatcgtc
      121 gctgaacgca ttgtggagcg catcatcgag cgcgtgaggc tcaagaccga gcgcgacaaa
      181 cttccgaacc ggcgcaaagg ctatacgcag aaggccgttg tcggcggcca caaggtctat
      241 ctgcgcaccg gcgaattcga agacggacgg ctcggtgaga tcttcatcga catgcacaag
      301 gaaggcgccg ccttccgaag cttgatgaac aacttcgcca tcgccatcag ccttggcctg
      361 caatatggcg tgccgctgga cgaatatgtc gaagcgttca ccttcacgcg cttcgagccg
      421 gccgggtatg tccaggggaa cgaggcgatc aagaacgcga cctcgatcct cgactatatc
      481 ttccgcgagt tggccgtttc ctatcttggc cgcaacgatc tcgcccatgt ggacccctca
      541 gagattggcg caaccgtgat cggcggcggg gtagcagacg acaggccggg ccagaaagct
      601 tccgcgccgg tgatctatgc cgaccgctac gtcagtaggg gcctcacgcg cgggcttcac
      661 cacaaagttg tcatagttca ggatcagcct aaaactgagc gaatcagtag cgccggttcc
      721 tatgagacgc gtggcgcgct ggcttatgcc ctgagtacgg atgacgagaa cgttccgtta
      781 gggtcttcca atgtaaacgc gcgcgatggg tcgagtgccg cgttgtctgc ttcccatctt
      841 acagtttcca atggagccaa acccggcaag gccgagttgc tcgcggaggc acgcgcaaag
      901 gggtacgagg gcgaagcttg cggcacctgc ggtaacttca ctctccttcg caacggaact
      961 tgtatgaagt gcgatacctg cggcggcact acgggctgtt cataaaccca tccgctgctt
     1021 ggttggggtt ctgcgcctcc ttctgcgcgg agccccggca tatcgccctt cgacacagga
     1081 agggcacgag agcacgacat ggcgctttgg acactcaatc cgccgcaaca ggaagacatc
     1141 tataccgttg tctatttcct gagggatacg ctgagttcgg cccttgaaga tgcttggttt
     1201 tttgagaaga ccgcgctgcc gaaggctgat gtgaagggcg cgggccacgc tgaaatcgct
     1261 gcccgcctcg gtcgcttccg cgacgggtgc aaaaccttcc aggagcggga agccctcatg
     1321 ctgatgaagc tttcccgcgc tcggatctgg gccgcggaac tgagaaagat ggcgccagaa
     1381 gttcagcacg acgtcgacca gtttctcgat gcaacagagg cctgcgacga gatgcaggcc
     1441 gagtttgtgc gagatgccca ccgcatgttc aatggaggcg gcttgctttc caggttcctc
     1501 gcccggcgga agccggaggc cgaggagcgc caaagccatt gcgcgcatga cggcagctac
     1561 cgggtcggcg gccgcacaga gctttttgaa ctgcgcgctg cttgcgaagt cttcctggcg
     1621 cagatcaatg atggcttctt cccgtcaaac catccagaga acgcggcgct gacgaagccg
     1681 ctggtcgcct atctggaagc gccgcaggag ccagcaccgg ccgtggttcc ggaggtgcat
     1741 tagcctgagc aaacacttcc gggcgccttc ccaaatagcc gggtttccag cgcatcgtgt
     1801 gttcggacac ggttacccaa atgcttggtt gtatgcaatg tgcgaatggc ggcatctgct
     1861 tgtcaacttg tcccttacgc ttgcgcccca cattccttat tctgaaagat cctatcggaa
     1921 agcgcttttc cgacaggtta acgcactcca gccattatcc agcccttggt attgcgagga
     1981 aaaattccga acggaacgcc atcgccgccg cctctgccgc ggctatttcg gccgcggcac
     2041 ctggttaatc tgcgaagccg aagcatattt aacggacttt tgctcgtccc agtaaatgaa
     2101 aaaattccgg cattaaattc tatttaatgg gcactttgtt aagcacaaaa ggctgatcat
     2161 tttcagaagc ttagggtagc ttgcttgcgt ccaaaccaaa ggcgcgacgc tcctcgtttc
     2221 gcgccggata aaagagtttt taaccaaaca catggcggga aataaatccg gccggctcct
     2281 gttaaccacc
//
LOCUS       DATMGX010000116         5092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_31306, whole genome shotgun sequence.
ACCESSION   DATMGX010000116 DATMGX010000000
VERSION     DATMGX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31306"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VNR65_01920"
     CDS             complement(<1..454)
                     /locus_tag="VNR65_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWJ17461.1"
                     /translation="MSNPKHKTPPFLRAMDFQYGVLERIAPGLRRLVCNNPSPFTFKG
                     TNLYVIGEGRVAVVDPGPASGEQLDVLCDALKGETITHILVTHCHADHSGAADALKVR
                     TGAPICGMPRGASAPSAGAKGPSGRGFIIPVAFDMALRHGSCIEGDGWE"
     gene            586..1260
                     /locus_tag="VNR65_01925"
     CDS             586..1260
                     /locus_tag="VNR65_01925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HWJ17462.1"
                     /translation="MTKTDIFSAVDGYISGLFAKEDTALSAAVQAMSEAGLPEISVSP
                     VLGKFLTMLAKLARARRILEIGTLGGYSTIWLGRALPEDGCLLTLEIDPLHARIARKN
                     LARAMLFGTVEVREGPAQDLLTKLIAAGEEAFDLIFIDADKEAYLEYFDLSLKLSRPG
                     TLIVADNVVRYGAVIDAHAGQSDSRVAGIQRFLDALAKHPGVDATIIQSVGIKGHDGL
                     ALAIVK"
     gene            1485..1766
                     /locus_tag="VNR65_01930"
     CDS             1485..1766
                     /locus_tag="VNR65_01930"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HWJ17463.1"
                     /translation="MERKLEPEGEKRENMSDAELEKRSSTLGKAFKVSTELVAGVFVG
                     GFVGWALDNLLMPKLGVNTTPLFLIVFLLLGIAAGFLNVFRAAREMGEK"
     gene            1802..2611
                     /locus_tag="VNR65_01935"
     CDS             1802..2611
                     /locus_tag="VNR65_01935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004482.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWJ17464.1"
                     /translation="MIDWFISAAHAAAAEGGQHGGGGFNPMHQFVIDRYYPISLFGYD
                     ASFTNSALWMVMATTVVSALLLYSMSGASAVPNRLQSVSEITYEFVANMVRQNAGNAG
                     MPYFPIIFTLFMFVLAMNLIGMIPLPGLGAFTVTSHIIVTFALAAFVFIGVTLIGIFK
                     HGFGFLKLFVPSGVPVWLLPLITVIEIISYFTRPISLSVRLFANMMAGHTMLAVFGSF
                     VVGLGIIGGWVPFIFMVLLMGLEVLIAFLQAYVFAVLSCIYLNDALHASEH"
     gene            2658..2885
                     /locus_tag="VNR65_01940"
     CDS             2658..2885
                     /locus_tag="VNR65_01940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HWJ17465.1"
                     /translation="MDPAAAKLIGAGLACSALIGAGVGIGSIFGNFLQGALRNPTAAP
                     GQFPNLLLGFALAEATGLFGLVIALILLFVF"
     gene            2946..3449
                     /locus_tag="VNR65_01945"
     CDS             2946..3449
                     /locus_tag="VNR65_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17466.1"
                     /translation="MPAKSGMPQLVPTDFAPQLIWLAIIFTLFYFVLSQLALPRIERV
                     LKDRKAKIGGDLQAAREAQHNADKEAERYEAEITAAKAKGHGTIRASREKLGAELNEK
                     RGVLDGQLAEKTAETEKRVQGLLERASGQMEAMTAGVVSDIVKELAGIEVSDKEVQAA
                     LRQRSKE"
     gene            3455..3952
                     /locus_tag="VNR65_01950"
     CDS             3455..3952
                     /locus_tag="VNR65_01950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HWJ17467.1"
                     /translation="MNPAIFSDPETWVAIAFILFVGVVLYYKLPGRVVKALDQRSAAI
                     ARELEEARKLRVEAEALLADYKQRSKNAQVEAEEILAQAEREAASYAKEARAAFDEMI
                     ARRLNVAEQKIKLEEEKARKQIRAQAAELAVSAAEQLIEQKMSGAVAENTITTSLDRI
                     KKRLH"
     gene            4075..4761
                     /locus_tag="VNR65_01955"
     CDS             4075..4761
                     /locus_tag="VNR65_01955"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HWJ17468.1"
                     /translation="MSALAAAASWFFLLHMVPSTPLRPRSVAIVGEGAYTAVFSILSL
                     IAIYWLSKQFNIAPYGDKLWMVPGWWPWVQAVLILFSFILMFGGFLTPNPATPVGAQA
                     FENPNLADGIFAITRHPVMWGVAIWAVTHMISQATWRGFAFFGAFAATALIGSWLQQN
                     RKRNTVSGWSAFEAKTSFFPFAAILQGRAKFSPRAIGWKPIVIALIAWAAILHFHFWL
                     FSVKPLSLSS"
BASE COUNT         1021 a         1504 c         1466 g         1101 t
ORIGIN      
        1 cctcccatcc gtcgccctcg atgcagctgc catgccggag agccatgtcg aacgcgacgg
       61 gtatgatgaa gccccgcccc gacggcccct tggctccggc agaaggggcg ctcgcgcctc
      121 gcggcattcc acaaattggc gcacctgtgc gcactttgag cgcgtccgcc gccccgctgt
      181 ggtcggcgtg gcaatgcgtg acgaggatat gcgtgatcgt ctcgcccttc agcgcatcgc
      241 acagcacatc gagttgctcg cccgacgccg ggccaggatc gactacagct accctgccct
      301 cgccgatcac ataaagattt gtgcccttga aggtgaaagg gcttggattg ttgcaaacga
      361 ggcgccgcaa gcccggtgcg atccgttcca gcacgccata ttggaaatcc attgcgcgca
      421 agaagggcgg cgtcttgtgt ttaggattgc tcaaggaaac ctcgcgacat ctctagccgc
      481 agaaaacttt ggccgtgcgc ccggtttgtc aaatacaaag aggcatggcc ttggacaaaa
      541 ccgccgtgga agccgcttcc ccaccggcag cgcaggagct ttggcatgac gaagaccgat
      601 attttcagcg ccgttgacgg atacatctca ggcttgttcg ccaaggagga tacagcgctg
      661 tccgctgcgg ttcaggcaat gagcgaggcc gggttgccgg aaatcagcgt ttcgccggtg
      721 cttggcaaat tcctcaccat gctcgcgaag ctcgcccgcg cgcgccgtat cctggaaatc
      781 gggacgctcg gcgggtatag cacgatctgg cttggccgcg cgcttcccga ggatggctgc
      841 ctcttgaccc tggaaatcga cccgcttcac gccaggattg ctcggaaaaa cctcgcccgg
      901 gccatgcttt tcggcacggt ggaagtccgc gaggggccgg ctcaagacct tctgacgaaa
      961 cttatcgcag caggggaaga agcgttcgac ctcatcttca ttgacgccga caaggaggcc
     1021 tacctcgaat atttcgatct gtcgctcaag ctgtcgcggc ccggcacgct gatcgtcgcc
     1081 gacaacgtcg tccgctatgg tgccgtcatc gacgcgcacg ccggtcaatc ggattcgcgc
     1141 gtcgcaggca tccagcgctt tctggacgcg cttgcgaaac atcccggcgt tgatgccaca
     1201 atcatccaaa gcgtcggcat caaaggccat gacggcctcg ctcttgcgat tgttaaataa
     1261 taattcgcct tccgcctaag gatgaatgcc acttaaggac gcggaagggc gacgcaggct
     1321 tgacttcgct tcatacgctg gcttaagaaa acagcgagaa attcctacgc gcttgttatt
     1381 cccaccgcga tcgggttccg atgtcttcca ccggcgacgg cgaccgagat gaacgtaagg
     1441 cttcgcccga gtcaagcgat cgcttgagag agcggcttcg cgagttggag cggaagctcg
     1501 aaccggaggg tgagaagcgc gaaaacatgt ccgatgcaga gctcgaaaag aggagttcga
     1561 cactcggcaa ggcattcaag gtttccaccg agctggtggc gggcgtcttc gttgggggct
     1621 ttgtcggctg ggcgttggat aacctgctga tgccgaagtt gggggtcaat acgactcccc
     1681 tcttcctgat cgtgtttctt ctgctcggca tcgctgccgg gtttctgaac gtcttccgcg
     1741 cggcgcgcga gatgggtgaa aagtaaaagc gaccgggcat tcggcgccgg cagggattgc
     1801 tatgatagac tggttcataa gcgcggcgca tgccgcggcg gcggaaggcg gccagcatgg
     1861 ggggggcggt ttcaacccca tgcatcagtt cgtcatcgac cgttattatc ccatatctct
     1921 gttcggctac gacgcgtcgt ttacgaactc cgcgctgtgg atggtcatgg caacgacggt
     1981 ggtctcggcc ctgttgcttt acagcatgag cggtgcatcc gcggtcccga accggctgca
     2041 atccgtaagc gaaatcacct acgagttcgt ggcgaacatg gttcgccaga atgctggcaa
     2101 cgccgggatg ccatatttcc cgataatctt cacgctcttc atgttcgtgc tggcgatgaa
     2161 tctgatcggc atgatcccgc tgccggggct cggcgcattt acggtcacga gccacatcat
     2221 cgtgactttc gcgctcgcag cctttgtctt tatcggcgtg accttgatcg gcatctttaa
     2281 gcacggcttc ggcttcctga agctgttcgt accgtccggc gtgccggtct ggctcctgcc
     2341 gctcatcacc gtgatcgaga ttatctccta cttcacgcgg cccatcagcc tctcggttcg
     2401 tcttttcgcg aatatgatgg ccggccatac tatgctagct gtgttcggca gttttgtcgt
     2461 agggctaggc atcattggcg gatgggtacc gttcatcttc atggtcctgc ttatggggct
     2521 ggaagttctg atcgcgttcc ttcaggctta cgtcttcgcg gttctctcct gcatctatct
     2581 gaacgacgcc ctccacgcat ccgagcacta accccttcgc aacctctcaa ccgatcaacc
     2641 gaataggaga aattgtcatg gatccagcag cagccaagct cattggcgcg ggcctcgcct
     2701 gctcggccct catcggcgcc ggcgtcggca tcggcagcat cttcggcaac ttccttcagg
     2761 gcgcgcttcg caacccaaca gcggctcccg gccagttccc gaacctcctg ctcggctttg
     2821 cgctcgccga agcaaccggt ctgttcggcc tcgttatcgc gctcatcctc ctgttcgtat
     2881 tctaatgttg aagattggcg ttcgtcgaac gacggcgcca tcgtcagcgc atagggggtc
     2941 cgatcatgcc tgcaaaatca gggatgcctc agctagtacc gacagatttc gccccccaat
     3001 tgatttggct ggcgatcatc ttcacgcttt tctatttcgt gctgtcacaa ctcgcccttc
     3061 cccggattga gcgcgtcctc aaggaccgca aggcgaaaat cggaggcgat cttcaggcgg
     3121 cgcgtgaggc ccaacacaac gccgacaaag aggcggagcg ctacgaggcc gagattaccg
     3181 ccgccaaggc gaagggccac ggcacgatcc gcgcttcacg agaaaagctt ggtgccgaac
     3241 tcaatgaaaa gcgcggtgtc cttgacggcc aactcgccga aaagacagcg gaaacggaga
     3301 aacgcgttca gggtcttctt gagcgggctt ccggacagat ggaagcgatg accgccggag
     3361 ttgtgagcga catcgtcaaa gagcttgctg gaatcgaagt cagcgataaa gaagtgcagg
     3421 cggctctgcg tcagaggtcg aaggagtagc gagcatgaac cccgccatat tctcggatcc
     3481 ggaaacctgg gtcgcgatcg ccttcattct tttcgttggc gtcgtccttt attacaagct
     3541 gcccggcagg gtcgttaaag cgcttgacca gcgttctgcc gcgattgcgc gtgaattgga
     3601 ggaagcccgg aagcttcgcg tcgaagccga agcgctgctt gccgattaca agcagcgctc
     3661 aaaaaatgct caggtcgagg ccgaggaaat cctagcccag gccgagcgcg aggcggcctc
     3721 ctatgcaaaa gaagcacgag cagccttcga tgagatgatc gcccggcgtc tgaatgtggc
     3781 ggagcaaaag atcaagcttg aggaagagaa ggcgcgcaag caaattcggg cgcaggctgc
     3841 ggagcttgcc gtttccgcgg cagagcagct gatcgagcaa aagatgtctg gagcggtcgc
     3901 agagaacacg atcaccacca gcctggatcg aatcaagaag cggctgcact agagagcgtc
     3961 cgaaaaagca aaacaaaaac ttagagcccg cttttgatcc catcaagaac ggataggctc
     4021 taaagagcgg cttcttcgcg ggccagcatc agcaattcat tgcaggagga agctttgagt
     4081 gcactggcgg cggcagcgtc ttggtttttc cttctccaca tggttccttc gacgccgctt
     4141 cggccgcgct ccgtcgcgat tgtaggagaa ggcgcttaca cagcggtttt ctccatccta
     4201 tcgctgatcg cgatttattg gctctcgaag caattcaaca ttgcgcctta cggcgataag
     4261 ctctggatgg tacccggctg gtggccatgg gtgcaggcgg tgctgatcct tttttccttc
     4321 atcctcatgt tcggcggctt tttgaccccg aaccctgcta cgcccgtcgg ggcgcaggcg
     4381 ttcgaaaatc ccaacctcgc tgacggcatt ttcgcaataa cccgccatcc tgtcatgtgg
     4441 ggggtcgcaa tctgggctgt cacgcatatg atttcgcagg ccacttggcg tggttttgcg
     4501 tttttcggcg cattcgctgc caccgcgctg atcgggagct ggctgcagca gaaccgcaaa
     4561 cgcaacaccg tttcgggctg gtcggccttc gaggcaaaga cctcgttctt tcccttcgcg
     4621 gcgatcttgc agggtcgcgc caagttcagc ccaagggcca tcggatggaa gcccattgtc
     4681 atcgcgctga ttgcctgggc cgccattttg cattttcact tctggctttt cagcgttaaa
     4741 ccactttcgc tttcctcgta agagaccagc gcactgccgt tagatcaacc cgctcataat
     4801 cggaatcgat aagggcggga aaggtgatcg tttctaaagt gctagagcag ctaactcctt
     4861 tgctcaacag gccctagagc gtgtcgaaat tattcggagt tcccttattg tcatggccgc
     4921 cctccgaggc ggccatccag gataacaaat tccatctttt tggattttca gactggatgg
     4981 ccgggtcaag cccggccatg acattggttc cagctaatcg ccaacacgct tttagtggcg
     5041 gaagtggcgg actcccgtaa acaccatggc gaggccccgc tcatcggcag cc
//
LOCUS       DATMGX010000117         2410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_275064, whole genome shotgun sequence.
ACCESSION   DATMGX010000117 DATMGX010000000
VERSION     DATMGX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275064"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1623)
                     /locus_tag="VNR65_01960"
     CDS             complement(97..1623)
                     /locus_tag="VNR65_01960"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HWJ17469.1"
                     /translation="MDIGFFGHFVMNGLSLIASLFVLAVGVFSLMIAVMFIADVSQTK
                     DAIRRNYPVIGRFRSLFTTLGEFFRQYFFAQDREELPFNRAERDWVYRSSTGADNTVA
                     FGSTRSLLPVGTIIFVNCPFPTLEEHAFEAPEITAGPYTPAPYSFSSIINISAMSFGA
                     LSVPAVRALSKGAAMAGCWLNTGEGGLAPYHLEGRCDVIFQIGTAKYGMRTLDGQLDE
                     ARVREIAGLSQVKMFELKLSQGAKPGKGGILPAIKVTPEIAKIRGIPVHTDSISPNRH
                     PEIDSVPDLLDQIARIRDLAGKPVGFKAVLGAYGWLETMCKEIHKRGIESAPDFITID
                     SGDGGTGAAPLPLMDNVGLPIKEALPMVIDILCQYDLRPRIKVVASGKLITPAEVAWA
                     YCAGADTVNSARGFMFALGCIQSLKCNKNTCPTGITTHNRRLQHGLNPEEKSVRVKNF
                     VERMRSEVGTIAHSCGVPHPRALRRYHCRIVQPNGRSIPMNELYPSMIPVEARFKDGR
                     "
BASE COUNT          532 a          669 c          704 g          505 t
ORIGIN      
        1 aattcccgcc tccagcgtag tcaggcttta tgaggcccgg cggtagacgc ggaggggccg
       61 agaggcgtgc cggcgccaat cctgaaggca acgaacttag cgaccatctt tgaagcgtgc
      121 ttccactgga atcatggacg gatagagctc gttcatggga atcgaacggc cattgggctg
      181 cacgatgcgg caatgatagc gtcgcaacgc ccggggatgg ggcacgccgc aggaatgggc
      241 gatggtgccc acttctgagc gcatgcgttc gacgaagttc ttgacgcgca cggatttttc
      301 ttctgggttc aacccatgct gcagccggcg gttgtgcgtc gtgatgccgg ttgggcacgt
      361 gttcttgttg cacttcaaag actggatgca gccaagggcg aacataaacc cgcgcgcaga
      421 attcaccgta tcggctccgg cacaataagc ccatgccacc tcggcaggcg taatgagttt
      481 gcccgatgca acaaccttga tgcgcgggcg cagatcgtat tgacacaaaa tatcgatcac
      541 catcgggagc gcttccttga tgggcaggcc gacattatcc atgagcggca aaggcgcagc
      601 gcccgtgcca ccgtcgccgg aatcgatggt gatgaagtcg ggcgcgcttt cgatgcctcg
      661 cttgtgaatt tccttgcaca tcgtttcgag ccagccgtag gcgccaagga cggctttgaa
      721 gccgacgggt ttgcccgcca agtcgcggat gcgcgcaatt tgatcgagaa ggtcggggac
      781 gctgtcgatt tcgggatggc ggttgggtga gattgaatcg gtatgaacgg gaatcccgcg
      841 gattttagcg atctcgggtg tgaccttgat ggcgggcaaa atgccgccct tgccaggttt
      901 tgcgccctga ctgagcttca actcgaacat cttcacttgg gaaagccctg cgatctcgcg
      961 aacgcgggct tcgtcgagtt ggccatcgag tgtgcgcatc ccgtatttgg ccgtgccgat
     1021 ctggaagatg acgtcgcatc tgccttcgag atgataaggc gccaatcctc cctcgccggt
     1081 attgagccag caaccggcca tcgccgcgcc ctttgaaagc gcccgtacgg ctggcacaga
     1141 gagcgcaccg aagctcatgg cggaaatatt gatgatcgaa gaaaagctgt agggagccgg
     1201 cgtatagggg ccggccgtaa tctcgggtgc ttcgaacgca tgttcttcca gcgtcgggaa
     1261 tgggcaattg acgaaaatga tggtgccgac ggggagcaag ctgcgcgttg agccaaacgc
     1321 aaccgtgttg tcggcgccgg ttgacgagcg ataaacccaa tcccgttcag cacggttgaa
     1381 cggcaattct tcccgatcct gtgcgaagaa atactgccgg aagaattcgc ccagcgttgt
     1441 gaagagagag cggaagcggc cgatgaccgg ataattacgc cggattgcgt ccttcgtctg
     1501 gctcacatca gcaatgaaca tgaccgcaat catgagggag aaaacgccaa cagccagaac
     1561 gaagagggag gctatgagac tgaggccatt cataacgaaa tggccaaaaa aaccgatgtc
     1621 caagggtcgc tccccgcagc cggtgaaaat ttcgccctgc gcctgtctta gctgcgcttc
     1681 aaaggccgct gaaccagtta tacccgccct cgtcccaata gccgccggga tagacgttca
     1741 tcacctccat ggcgtagatc cattttggct gtttgaagag aacccgagcc cgcatcggtt
     1801 acactctcaa tctcaaagaa acttttctca atacgtcgct caagcgcaga gtcatgtcaa
     1861 agttgatatg cccagaacaa cgaaacgtga cgtgccgtac gataagagca ttgctcatgt
     1921 aactcttgaa ctctcaaacg cttatcgcgc tgttgcacgc gtccggcgtc tcagtggaag
     1981 agcgtttagt cacaggcacg gcggcgccaa aagctgcgat aaccttgagc gccctcagtc
     2041 gccccatagt tcgcttaact gctatgctca aactctgaca gacgctgcac acgcaacagc
     2101 taagtgcgcc gccatattgc tgttctagca ggcttttctg aatgagcggc agccgttggc
     2161 tgaattctct gtttgcgtct gagcactaga tttacaacaa aatcggcccc tatgcaggct
     2221 tcgccccttc ggcggcttct tggtgagaag cgggcaaagg ttgccgaaaa tttgcgatgg
     2281 tgagacccgt ggctgggcga tcgcaggttc cttgcgagac aaggcctctt cttcagtcct
     2341 gtccttcaac gcttgtggcg cgggtgacca gagcctcgaa cttggcatcc agcgccttcg
     2401 ctgccgcgag
//
LOCUS       DATMGX010000118         2300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_249095, whole genome shotgun sequence.
ACCESSION   DATMGX010000118 DATMGX010000000
VERSION     DATMGX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249095"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1901)
                     /locus_tag="VNR65_01965"
     CDS             complement(129..1901)
                     /locus_tag="VNR65_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWJ17470.1"
                     /translation="MTYAPLALLGAAVASYGQDQKPVELPPGAPPAFGTAPPVGPEVS
                     PATFAQAEKLVEVELSSEELAQAAGNWRSAMAPLYERRMGPHKVALEATLAPGTRWDP
                     VMPSEKAGPASNQFVRSKTDPGPLPSKDEDIAYAPVTQLSLWIQQRKLSSERLTQIYL
                     SRLEKFNPKLRCVITLTRELALAQAKRADQEIAAGKYRGPLHGIPWGAKDLLDTAGIP
                     TTYGAEPFRNRIPTTNAVVVQRLNDAGAVLVAKLSLGALALNDIWFGGQTMNPWLLEE
                     GSSGSSAGPGAATAAGLVGFAIGSETGGSIVSPSMRCGVTGLRPTFGRVARTGAMTLC
                     WSLDKLGPMARSVEDTMLVLNAISGPDPGDVSSVPSKLEFDAGASVQGLRVGYFPAWM
                     KQNPATDVDRAALEAVKKVGMVPVEVAIPDWPYDSLDLILFAEAAAAFEELTLDGGLN
                     QLKMQVPDAWPNTFRQSRFLSAVDFVQTDRFRRKVAMEMERVFSQVDLLLVPSLRDEM
                     LTITNFTGQPSLTLRAGFVEVAEARSDWAPDPAHPLPKFSPPRRVPHGVTLIGQLFDE
                     GTLGQGGIALERAFNVAGERPSGF"
     gene            2156..>2300
                     /locus_tag="VNR65_01970"
     CDS             2156..>2300
                     /locus_tag="VNR65_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWJ17471.1"
                     /translation="MKISIIGAGQIGGTLARRFTALGHQVFVANSRGPETLAGLARET
                     GATP"
BASE COUNT          434 a          710 c          715 g          441 t
ORIGIN      
        1 ccgcgctagt ttagtcgatt gcggtcagaa cgacgtagct ggcgctgcgg aagtagccac
       61 ggagtggtgt cagctccacc ggctattccg aagttcgatt cctgtgatcg aaaatggatt
      121 gcagccgatt agaacccgga aggacgctcg ccagccacgt tgaacgcgcg ctccagcgca
      181 atgccccctt gccctagagt tccctcgtcg aagagctggc cgatcagcgt gacaccgtgg
      241 ggcactcgtc gcggcggcga gaacttcggc agcggatgag cgggatcggg cgcccaatcg
      301 ctgcgggcct ccgccacttc gacaaagccc gcgcgcaagg tgagcgaagg ctggccggta
      361 aagttggtga tggtcagcat ttcatcccgt agcgaaggca cgaggagcag gtccacctgc
      421 gagaagacac gctccatctc catggccacc ttgcgccgga agcgatcggt ctgcacgaag
      481 tccactgccg acagaaaccg cgactggcgg aaggtgttgg gccaggcgtc gggcacctgc
      541 atcttgagct ggttgagccc gccatcaagt gttagttcct cgaacgcagc agcggcttcg
      601 gcgaacagaa tcaggtcgag cgagtcataa ggccagtctg ggatagccac ctcaaccggc
      661 accatgccaa ctttcttgac tgcctccaat gcggcgcgat ccacgtccgt ggcgggattc
      721 tgcttcatcc aagcgggaaa gtagccgacg cgcagtccct gcacgcttgc ccctgcatcg
      781 aactcaagct tgctgggcac gctagacacg tcgcccgggt cggggccgga tatagcgttc
      841 agcaccaaca tggtgtcctc tacgctgcga gccataggcc caagtttgtc gagcgaccag
      901 cacagcgtca tggcgccagt acgcgcgacg cggccgaagg ttggccgcag tccggtgact
      961 ccgcagcgca tggaaggact cacgatgctt ccaccggttt cgctgccgat cgcgaagcct
     1021 accaatcctg ccgctgtggc tgcaccggga cctgcgctcg agcccgagga gccttcctcc
     1081 agcaaccagg ggttcatggt ctggccgccg aaccagatgt cattgagcgc cagcgcgccc
     1141 aaactcagct tggcgaccag caccgcaccc gcatcgttca gtcgctgaac gacgaccgcg
     1201 ttggtggtgg ggatgcgatt gcgaaagggc tcggcgccgt aggtggtggg gatgcctgcc
     1261 gtgtccagaa ggtccttggc tccccacggg atgccgtgca gcggcccgcg atatttcccg
     1321 gcggcgattt cctgatcagc tcgcttggcc tgggcaaggg ccagctcacg ggtgagcgtg
     1381 atgacgcaac gcagcttggg attgaacttc tccagacgag agagatagat ctgggtaagc
     1441 cgctccgagc taagcttgcg ctgctgaatc cagagcgaga gctgggtcac aggtgcgtag
     1501 gcaatgtctt cgtccttgct tggcaacggc ccgggatcgg tcttgctcct gacgaattga
     1561 ttgctcgccg gtccggcctt ctcgctgggc atgactgggt cccagcgcgt tccgggcgca
     1621 agcgtcgcct ccaaagctac tttgtgcggt cccatccttc gctcgtagag cggagccatg
     1681 gcgctgcgcc agttgccggc tgcctgggcg agttcctcgg aactcaattc cacttccacc
     1741 agcttttccg cttgggcaaa cgttgcaggc gacacctcag ggccaaccgg aggcgccgtt
     1801 ccgaaggcag gaggcgcgcc cggcggtagc tcaacgggct tctggtcttg gccgtaagac
     1861 gcgacggccg cccctaacaa agctaggggt gcataggtca agaattgtct gcgtgatttc
     1921 gacatgtctc tcctgaagcg gaccgtcgcg aggtctggtc ccgagatgca cttgattggg
     1981 tcggcgcgac ctgctcacgt ttcgggatgc tccggaactt tcggaaattc catacctgcc
     2041 gatcaaccat cttgatcatc ttatactggg tgcttggttc accaggggaa ggtcccatgc
     2101 caaatgggct tcttagaccg agcgagtaat tagcggtact catcaaggag cagatatgaa
     2161 gatcagcatc atcggcgcag gccaaatcgg tggtaccctc gcccgtcgct tcactgcgct
     2221 gggccatcaa gtctttgttg ccaactcaag aggtcccgaa acgctggccg gcttggcccg
     2281 tgagaccggc gcgacccctg
//
LOCUS       DATMGX010000119         2256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_366268, whole genome shotgun sequence.
ACCESSION   DATMGX010000119 DATMGX010000000
VERSION     DATMGX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366268"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1287
                     /locus_tag="VNR65_01975"
     CDS             154..1287
                     /locus_tag="VNR65_01975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWJ17472.1"
                     /translation="MSVAAQSTRPPRSVRLFLCGDVMTGRGIDQVLIHPCDPTLHEDY
                     VASANDYVGLAEQATGPIPRRAEPSYVWGAALDELDRMQPDARIINLETAITRSDDYA
                     RKGINYRMSPENADCLSAAGIDCCVLANNHVLDWGPSGLVQTLATLERLEIKTSGAGR
                     NVIEAAAPATLDIADKARLLVFSFGDVTSGIPRSWAATADNPGVNLLTRLSEAEALRA
                     AEYILSIRQPGDLAVVSLHWGLNWGYEIPAEQQRFARTLIDRANVSIIHGHSSHHAKA
                     IEVYHKRLILYGCGDFLNDYEGIRGYEEFRSDLALMYFADIEPATGNLAAFEITPLQI
                     RKFRLDRPSKQDVEWLQRTLQRECQPFGTGIVLDPGGRLVLSH"
     gene            1640..2104
                     /locus_tag="VNR65_01980"
     CDS             1640..2104
                     /locus_tag="VNR65_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17473.1"
                     /translation="MEPFPVDIDPKQIVRWVIAEHRMTPSSLKIVARRTAEERQIPAR
                     AEFHLGDEEREDLSEIATVATLEIAPAHPSDGWLMTITVEDEIGPRGPGRGTTVEDDQ
                     QIDLETFYSQFIRPERGTATVAAHANDAAAEARISSLLASIENDRHGPGVGK"
BASE COUNT          473 a          691 c          681 g          411 t
ORIGIN      
        1 atagcgtcca tcgcgacccc ggcatcgatg tcgaagaagc cgcccgcgta gtcttcgccg
       61 cgcttgcagc gcgcctgcca gcggcggaac tggaggacgc caaggccgcg acgccggagc
      121 cgctgcataa tctttggcca agctgactag gccatgagcg ttgccgcgca atccactcgg
      181 ccaccgcggt ctgtgaggct gttcttgtgc ggcgacgtca tgacggggcg aggcatcgat
      241 caggtactca tccacccctg cgacccgacg ctccacgaag actacgtggc ctcggcgaac
      301 gattatgttg gtctggccga gcaggcgacc ggcccaattc cccgacgcgc cgaaccttcc
      361 tacgtctggg gcgccgcgct ggatgaactg gaccggatgc agccggatgc ccgtatcatc
      421 aatctggaaa cggcaattac ccgaagcgac gactacgccc gaaagggcat caattaccgc
      481 atgagccccg agaatgccga ctgcctctcc gctgcgggca tcgactgttg cgtgctcgcc
      541 aataatcatg tcctcgattg gggaccgagc ggtctggtgc agacattggc gacgctggag
      601 cgtctagaga tcaagactag cggggccggc cgaaatgtca tcgaagccgc agcgcccgcg
      661 acactggata ttgccgacaa ggcgcggcta ctcgtgtttt ctttcggcga tgtgacaagc
      721 ggcattcccc gcagttgggc cgccacggcg gacaatcccg gtgtcaacct cctgacccgg
      781 ctttccgagg cggaggcgct acgcgccgcc gaatatatcc tcagcatcag gcagccgggc
      841 gatttggccg tggtctcgct tcattgggga ttgaattggg gctacgaaat ccccgccgaa
      901 caacagcgct tcgcgcgcac gctaatcgac agggcgaatg tgtcgatcat tcatggccat
      961 tcatcgcacc acgcaaaggc catcgaggtc taccacaagc ggctcattct ctatggctgc
     1021 ggcgacttcc tcaatgatta tgagggcatc cgcgggtacg aagaatttcg cagcgacctg
     1081 gcgctgatgt atttcgcaga tatcgaaccg gcgaccggaa atctcgctgc gtttgagatc
     1141 acgcctcttc agatcaggaa gttccggctt gaccgccctt cgaagcagga cgtcgagtgg
     1201 ctgcaacgga cgctgcagcg ggaatgccag ccgttcggga ccggcatcgt gctcgatccg
     1261 ggcggccggc tcgtcctttc ccattgattc aggtcaaggc actccgccgg ttttcgtgct
     1321 taaagaattt tgccgttgtg tcgaggcaag attggcgaaa cacggcagtg aaccgtctaa
     1381 gggcgtggat ctgcttacgc catgaaccag tctgccgagg aagcatggcg gaggggcccg
     1441 tagctgccct gtccgagcac cccggaggag acttcggttc gacgggcggg aggcgcgggc
     1501 ccttgtgctt tgtaagaccc cggacgcgcc cgatcggata tccatcgaac ttctcctgaa
     1561 cgctttcctt tgcgccagat caacggcatg aaccgccgct cgtactaaac gaacagcaat
     1621 agctcggggg atatcccgaa tggaaccctt ccccgtagat atagatccca aacagatcgt
     1681 tcgctgggtc attgccgagc atcggatgac cccctcgtct ctgaagatcg tcgcacgccg
     1741 cacagccgag gaacgacaaa tccccgctcg cgcagaattt cacctcggcg acgaggagcg
     1801 cgaagacctg agcgaaatcg ctactgttgc aaccctggag atcgcgcccg cccatccgtc
     1861 cgacggctgg ctgatgacca ttaccgttga agacgagatc ggtccgcgcg ggcccggtcg
     1921 gggcacgacg gtggaggatg accagcagat tgatctggaa accttctaca gccagttcat
     1981 tcgccccgaa cgaggaaccg cgacagtcgc cgcgcatgcc aacgatgccg cggccgaggc
     2041 ccgcatctct agtctacttg cttcgatcga gaacgatcgg cacggccccg gcgttggcaa
     2101 gtaagagcgg gaattgaatg gagaggatcg atggaaagcg caacattccg aaaatggcta
     2161 atcgagcagg gctgtcgctt cgatacgcaa caggaagaac gcggagaagg tcacggaact
     2221 gtgaaaatcc accgcgaggg gcggactgct gaactg
//
LOCUS       DATMGX010000120         4192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_217273, whole genome shotgun sequence.
ACCESSION   DATMGX010000120 DATMGX010000000
VERSION     DATMGX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217273"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VNR65_01985"
     CDS             <1..307
                     /locus_tag="VNR65_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HWJ17474.1"
                     /translation="GGAPSVVSVFAGRIADTGQDPVPLMAEAVKIVSPHAGIELLWAS
                     PRELLNIFQANDVGCHIITATDSVLSKLKTVGKDLGEYSLETVRMFYDDAAHSGYKL"
     gene            335..1567
                     /locus_tag="VNR65_01990"
     CDS             335..1567
                     /locus_tag="VNR65_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWJ17475.1"
                     /translation="MIGGAGSTSNAEGRDVELLVPANDMAEPELSIVIPALNEELTIA
                     QFVDWCKEGLERANVRGEVLIIDSSTDATAEIAVTKGARVLKVPKRGLGRAYIDAIPF
                     IRGEYVLMGDADCTYDFRDLGPFMQSFHGEHEYVMGSRFRGYIEPGSMPPLHQYFGTP
                     ITTWLLNFLYGSDFSDIHCGMRGITRDALCRMDLHSQSWEYASEMVLKSVRMELRTAE
                     VPVRFLKDQEGRFSHHKRAGWFSPWHAAWINLRAMFIYGADFFLFRPGLLLLSIGLLL
                     TLPTTFGPVSLGPVTFSLYWMLLGLSLSVLGLHGFYMGALARVFFDYSGQITRRFLQM
                     FSYTRSVVVSAFAVVVGAALAVPLVSQYLRSGLRLPVNVAPASHMAVTGLLFVMGGAM
                     NFTFTLALHAAAANVQRR"
     gene            1551..2216
                     /locus_tag="VNR65_01995"
     CDS             1551..2216
                     /locus_tag="VNR65_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ17476.1"
                     /translation="MSNGDRKLLREGARDRSYGQDYRASVVDLFGVWLSARQICRYAG
                     SLADKELGDFGCGYHAAFVRRVLPELKHAVVVDFALSPDLKCFPNLTAIEGILPIVLT
                     QVPSSSLDIVLCISVLEHLWDPLTALQECLRIVRPGGTCVFNVPSWRGKRFLEYSAFR
                     LGLSPKDEMDDHKAYYDVRDFWPLLVRAGFRPSNIRCFSHKFGLNTFAVCRSTNDSTN
                     IAQ"
     gene            2213..3190
                     /locus_tag="VNR65_02000"
     CDS             2213..3190
                     /locus_tag="VNR65_02000"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWJ17477.1"
                     /translation="MTNYRRYFIVGGAGFIGSHFTDRLLADNSVAAVTLYDNFSSGRE
                     WHYEQHVRDPRFRVVRGDVKDIEALTAAMQGLDVVIHLASNPDIARAATEPDIDFREG
                     TYLTNNVVEAMRRSGTKAILYSSGSGVYGDLGEEEADEDHGPLLPISTYGASKLAGEV
                     LITSYCHMFDFTGRVFRFGNVVGPRQTHGVGFDFLRQLLKDPRKLKILGDGTQSKSYI
                     HVSDVVSAVLLAAERSTVAFAAYNVATGDYITVTEIAHLAVECAGLGPGSVEFEYTGG
                     NRGWKGDVPVVRLNTDRIRGLGWSCGASSREALRKSMLAMLPDLRSGRL"
     gene            3207..3989
                     /locus_tag="VNR65_02005"
     CDS             3207..3989
                     /locus_tag="VNR65_02005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ17478.1"
                     /translation="MSETNRDDWDIHWEDYGEGASLNPAQEYRRRLIVKLLGLRGGGS
                     GVRLLDIGSGQGDLAAEIRASFPAAEILGLELSHSGVEISRAKVPDARFVQCNLLEQT
                     EPPVDLHHWATHAVCSEVIEHVDDPVLLLSSAREYMRPECHLVVTAPGGPMSAFDKHI
                     GHRKHFRPDEIVGLLQQAGYRAEFAGKAGFPFFNLYRCAVILRGQKLVEDISAGNNSP
                     GTRMARFVMAVFHLLFRVNLQASPWGWQMVAKAMPLSRSNNG"
BASE COUNT          815 a         1130 c         1276 g          971 t
ORIGIN      
        1 tggtggcgct ccgtcggtgg tttcggtgtt cgccggaagg attgcggaca cgggccaaga
       61 tcctgttccg ctcatggctg aagcggtgaa aatcgtttcg ccgcacgcag gtatcgagct
      121 gctgtgggca agtccgcgcg agctgctgaa catcttccag gcgaacgacg ttggctgcca
      181 catcatcacg gcgaccgaca gcgtgctgag caagcttaaa accgtcggca aagatctcgg
      241 cgagtattcg ctcgaaacgg tgcggatgtt ctacgacgat gcggcccatt cgggatacaa
      301 actctaggcc ggctcaatcg tggagagttc ttgtatgatc ggtggtgccg gttcaacgtc
      361 caatgcagaa ggccgcgatg tggagctgct ggtccctgcc aacgacatgg ctgaaccaga
      421 attgtccatc gtgattccag cactcaacga ggaactcacg attgcgcagt tcgtagactg
      481 gtgcaaggag ggtcttgagc gcgccaatgt caggggcgag gtactgatta ttgacagctc
      541 tactgacgcc accgccgaaa tcgcggtgac gaaaggagca cgcgtgttga aggtgcctaa
      601 gcgtgggcta gggcgcgctt atatcgacgc catcccgttt attcgcggcg agtatgttct
      661 gatgggcgac gctgattgta cctatgactt ccgcgacctg ggaccgttca tgcaaagctt
      721 tcacggcgag cacgaatatg tcatggggtc gcgcttccgc ggctacatcg agcccggttc
      781 catgccgccg ctgcaccagt acttcggcac acccatcacg acctggttgc tcaatttcct
      841 atacggcagc gacttctccg acattcattg cggaatgcgc ggcataacac gtgatgccct
      901 gtgtcgcatg gacttgcact cacaatcatg ggaatacgcc tcagagatgg tgttgaagtc
      961 ggtccgtatg gaattacgta ccgcagaagt tccggtccgt tttctgaagg accaggaagg
     1021 gcgcttcagc catcacaagc gggcgggctg gttctcgccg tggcatgcgg catggatcaa
     1081 cctgcgggcg atgtttattt atggcgcgga tttttttctc ttccgtcctg gcctactgct
     1141 cttatcgatc gggctgttgt tgacgttgcc gacgactttc ggcccagtga gcctcggtcc
     1201 ggttaccttc tcgctctact ggatgttgtt gggactgtcg ttgtcggtac ttggattgca
     1261 tgggttctac atgggtgcgc tggcacgagt gttcttcgat tacagcggtc agatcacgcg
     1321 gcgtttcctg caaatgttct cctatactcg gtcagtcgtc gtcagtgcct tcgccgtggt
     1381 cgtcggcgcc gcgctagctg ttcctctggt ttcacagtat ctccgttccg gtttgcgatt
     1441 gccagttaat gttgccccag ccagccacat ggcggtaaca ggactgcttt ttgtgatggg
     1501 cggggcaatg aatttcacct ttacgctggc gctccatgcc gcggccgcga atgtccaacg
     1561 gcgataggaa gctactgcgt gaaggcgctc gcgaccgctc ctacgggcaa gactaccgag
     1621 cgtccgtggt ggatctcttc ggggtatggc tctctgcccg tcagatttgc cggtatgcag
     1681 gatcgctggc ggacaaagaa ctgggagatt ttggttgtgg ttaccacgcg gcgtttgtcc
     1741 gacgtgtatt gcctgagttg aagcacgcgg tcgtggtaga cttcgcattg tccccagacc
     1801 tcaaatgttt tccgaattta acggcgatcg aaggcatttt gcccatcgtg ctcacgcagg
     1861 tgccgtcgtc gagcctggac attgtgcttt gcatctctgt tctcgaacat ctatgggatc
     1921 cgttaacagc gctgcaggag tgcttgcgta ttgtacgacc tggaggaacg tgcgttttca
     1981 atgttccgtc ctggcgaggc aaacgctttc tcgagtattc ggcattccgg ctagggttaa
     2041 gcccgaaaga tgagatggac gaccataagg catattacga tgtaagggat ttctggcctt
     2101 tgctggtgcg ggcgggattc cggccgagca acatccgatg tttctcgcac aagttcggcc
     2161 tgaatacctt cgccgtctgc cggtcgacga acgacagcac gaacatcgca caatgaccaa
     2221 ttaccgccgt tacttcatcg taggtggagc cgggttcatc ggtagccact ttacagaccg
     2281 actgttggca gacaactcgg tggcagcggt aaccctgtac gacaactttt cctcagggcg
     2341 cgaatggcac tatgagcagc acgtgcgtga tccgcgcttc cgtgtggttc gcggcgacgt
     2401 gaaggacatc gaggcgttga ccgctgccat gcagggcctt gacgtcgtaa ttcatctggc
     2461 ctcgaatcct gatatcgcgc gcgccgcaac tgagccagat atcgatttcc gcgaaggcac
     2521 gtacctgacc aacaacgtag tcgaggctat gcggagatcg gggacaaaag caatcctgta
     2581 ctcttccggc agtggcgttt atggcgacct gggggaagaa gaagccgatg aggaccatgg
     2641 tccgctgctg ccgatctcca catatggcgc gagcaagcta gctggcgagg tactgataac
     2701 ttcttactgt cacatgttcg atttcaccgg tcgggtcttt cgtttcggca atgtggtcgg
     2761 accgcggcag acgcatggag tcggcttcga ttttctgcgg cagctgctga aggatccgcg
     2821 aaaactgaag atcctgggcg acggaactca gagcaagtcg tacatccatg tttccgatgt
     2881 ggtcagcgcc gtcctgctgg cggcagagcg atcaaccgta gcatttgcag cgtacaacgt
     2941 ggcgactgga gactacatta ctgtaaccga gatcgcgcac cttgccgtgg agtgcgcggg
     3001 tctggggccc ggatctgtcg agttcgagta caccggcggg aaccgcgggt ggaagggcga
     3061 tgtaccggtt gtccggctaa acaccgatcg cattcgaggc ttgggttgga gttgcggcgc
     3121 cagttcgcgg gaagcgctac ggaaatctat gttggccatg ctgccggacc taagatcggg
     3181 tcgactttag ccgcggcgga ggacgcgtga gcgaaacaaa tcgagacgat tgggacattc
     3241 attgggagga ctacggcgaa ggcgcatcct taaacccggc gcaggagtac cgccgccggt
     3301 tgatcgtcaa gctgctgggt ttgcgagggg gcggctcagg cgtccggcta ctggacatcg
     3361 gcagcggaca aggggacttg gccgctgaaa tccgcgccag tttccccgct gctgagattt
     3421 tgggattgga actcagtcac agtggcgtcg aaatttccag ggcgaaagtg cccgatgccc
     3481 gcttcgttca atgcaaccta ttggaacaaa ccgagccacc cgtggatttg caccattggg
     3541 caacccacgc tgtgtgctct gaggtcatcg aacatgttga cgacccggtt ctgctcctca
     3601 gtagcgcgcg cgaatacatg cgtcctgaat gtcacttggt agtgacggct cctggcggac
     3661 ccatgtcggc gttcgacaag cacatcggcc accgcaagca ttttcggcca gacgagattg
     3721 tcggcctgtt gcaacaggcg gggtatcggg cggagttcgc ggggaaggcc ggttttccgt
     3781 ttttcaacct ttaccgctgc gccgtaattt tgcggggtca gaagctcgtt gaagacatca
     3841 gcgcggggaa caactctcca ggcacacgca tggcgcgatt tgtcatggct gtgtttcact
     3901 tgctcttccg tgtgaatctg caagcttcgc cgtggggatg gcagatggtc gcgaaggcca
     3961 tgccactgtc gcgatcaaac aatggataac atagcaacga ctcctaaacg gacgcgcgta
     4021 acagcagcca tgttgtttgt tgcggtgtcc tgctgttacc tgtcgctatc tcccggatcg
     4081 atcgctggcc agggttacgc cagcgaggag atggattccg gtttgcggat gctctccgaa
     4141 atgacctcac ggctgaaggg gcttccggtg tccacgatgg tgtggtcccg cc
//
LOCUS       DATMGX010000121         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_274444, whole genome shotgun sequence.
ACCESSION   DATMGX010000121 DATMGX010000000
VERSION     DATMGX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274444"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VNR65_02010"
     CDS             <1..1143
                     /locus_tag="VNR65_02010"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HWJ17479.1"
                     /translation="FFVKDYLLGQEPMPFDILYWNSDSTNMPAAMHSFYVRNMYLYNR
                     LREPNGITLAGEGIDLRRITIPQYFLSASEDHIAPWKSTYAWTQLVSGPVRFVLGGSG
                     HIAGVMNPPAAKKYCYWTNNKIAATPDEWIAEAERHEGSWWKDWDQWVGQFAGNSVPA
                     RQPGDSALKPIEDAPGSYVRVRPNSSGHVYNVELTRAAVARCSSKAEAVQAAIKEFEK
                     ERKAARWDVVADGYDVLERGPKPLQTERLRQRAHELWEQEGRPEGRQDEHWARACREL
                     QMEEQDSFVENTGVAEQEKPTPIIPAEKAEKASAPFNEEVKGTNEGVEDTTMWKSQDK
                     EPEKQIKIVLADEKPDPLGRKIGSLPLNQMKAGYDYALGKGGKRRA"
     gene            complement(1596..2297)
                     /locus_tag="VNR65_02015"
     CDS             complement(1596..2297)
                     /locus_tag="VNR65_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ17480.1"
                     /translation="MLKVDHLQAAYGKVQTLWEVGFEVPKGEIVALLGANGAGKTTTL
                     KVLSGLLRPQAGSIMFDGERIDGCSPTEIVRRGIIQVPEGRALFREMSVIDNLLMGAY
                     ATPKVGRGMRLEQIFGIFPRVKERRKQLAGTLSGGEQQMVAIGRGLMADPKLLMLDEP
                     SLGLAPLMVEEVFGVIKEINASGVTVLLVEQNTHHALALSSRGYVLEAGRVVLSGSGR
                     ELLANTAVRKAYLGL"
     gene            complement(2290..>2488)
                     /locus_tag="VNR65_02020"
     CDS             complement(2290..>2488)
                     /locus_tag="VNR65_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ17481.1"
                     /translation="ELVQQIRQSGITVILVEHHMRAIMKISDRVIVMNHGAKIADGPP
                     KDIVQNRDVIVAYLGEGASDA"
BASE COUNT          544 a          736 c          754 g          454 t
ORIGIN      
        1 ttcttcgtca aggactatct cctgggccaa gagccgatgc cgttcgacat cctgtactgg
       61 aactcggact cgaccaacat gccggcggcg atgcacagct tctatgtgcg caacatgtac
      121 ctctacaatc gtttgcgtga accaaacgga atcacgcttg caggagaagg catcgatctt
      181 cgccgcatca cgattcccca atatttcctg tcggccagcg aggaccacat cgctccgtgg
      241 aaatcgacct atgcctggac tcagctcgta tccggtccgg tgcgattcgt tctggggggc
      301 tcgggccata tcgccggggt gatgaacccc ccggcggcca agaaatactg ttattggacg
      361 aacaataaga ttgccgccac gccggacgag tggattgccg aggcagagcg ccacgaagga
      421 tcgtggtgga aggattggga tcaatgggtc ggccagttcg ccggaaattc ggtgccggcc
      481 cgccaacccg gagatagcgc gctcaagccg atcgaagatg ctcccggctc ttacgtgaga
      541 gtccgcccca atagtagcgg gcatgtttac aacgtcgagt tgactagggc cgcggttgca
      601 aggtgctcct cgaaggcaga agccgtgcaa gcggcaatta aagagttcga aaaagagcgt
      661 aaggccgccc gctgggatgt tgtcgcagac ggctacgacg tcttagagcg cggccccaag
      721 ccgctccaga ccgaacggtt acgccagcgc gcccatgagt tatgggagca ggaaggccgt
      781 ccggaggggc gtcaggacga gcactgggca agggcatgcc gcgagcttca gatggaggaa
      841 caggactcgt tcgtcgaaaa cactggggtc gctgagcagg aaaagccaac tccgatcatc
      901 cccgcggaga aagccgaaaa ggcgagcgcc cccttcaatg aagaagtgaa aggcacgaat
      961 gagggtgtgg aggacacgac gatgtggaaa agccaggaca aggagccgga gaagcagatc
     1021 aaaatcgttc tggcagacga gaagccagac ccgcttggaa gaaaaatcgg aagcttgcca
     1081 ttgaaccaaa tgaaggctgg ctacgattat gcgctcggga agggcggtaa gagacgcgcg
     1141 taatccgccc accgctcgct tgttgctgga aggcgaaggc gcgattgaga accatgaacg
     1201 gcggtattgg tcagaaatgc gaatgtttcg ccgtgggcgt gcttgctgcc ggatggaagc
     1261 cgcgttgtta gcgaattgcg gatcgtcaag gagacagtcg agaggattat gagtttatga
     1321 gtagccgcat ctccccgaca agcagcgcga ggtgacgccc gcatggggtc gaaagccgcc
     1381 gtgccggttt tctcgccacc cgatgctcgg caagtcggct tcgggatccc attgccgtct
     1441 tctccgaaaa ggccaagaca atccggccct tgacccgagc cgaccttcgt gcggacgtgc
     1501 aagcaatgaa atagaatgtc gggacagcga ctggctacag ggagccaacg ctggtcacat
     1561 tgggggcgct tctgcgctgc ataatcaccc ctatcctaca gcccgagata agccttgcgg
     1621 acagcggtat ttgccagcaa ctcgcgccca ctgcccgaaa gaacgacgcg gccggcttcg
     1681 agcacatagc cgcggctcga cagcgcgagc gcgtggtggg tgttctgctc gaccaggagc
     1741 accgttacgc ccgaggcgtt gatctctttg atcacgccga acacctcctc gaccatgagc
     1801 ggcgcaaggc ctagcgatgg ctcgtcgagc atcaaaagtt tcggatcggc catcagcccg
     1861 cgtccgatcg caaccatttg ctgctcgccg ccggaaagcg ttcccgccaa ctgcttccgc
     1921 cgctctttca cacgcggaaa gatgccgaag atctgctcca ggcgcatccc ccggccgact
     1981 ttcggcgtgg cataggctcc catcagcaga ttgtcgataa cgctcatctc ccggaacaag
     2041 gcgcgcccct ccgggacctg gatgatgccg cggcgcacga tttccgtcgg cgagcaccca
     2101 tcgatacgct ccccgtcgaa catgatgctg ccggcttgcg gccggaggag acctgacagg
     2161 accttcagcg tggtggtctt gcctgcgccg ttggcgccga gcagcgccac gatttcgccc
     2221 ttcggcacct cgaatccgac ctcccacagg gtctgcactt tcccataggc ggcttgcaag
     2281 tggtcgacct taagcatcag atgccccctc gccgaggtag gcgacgatga catcgcgatt
     2341 ttgcacaatg tccttgggcg gcccgtccgc aatcttggcg ccatggttca tgacgatgac
     2401 gcgatcggaa atcttcatga tcgctcgcat gtgatgctcg accaggatca cggtgatgcc
     2461 actttgccgg atctgctgca ccagttcg
//
LOCUS       DATMGX010000122         2303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_370677, whole genome shotgun sequence.
ACCESSION   DATMGX010000122 DATMGX010000000
VERSION     DATMGX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370677"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VNR65_02025"
     CDS             <1..272
                     /locus_tag="VNR65_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17482.1"
                     /translation="DCEKTGITACDPMQKMDAIVQAEDSPSLIARLRFPLDPKEETWR
                     PTSQRAVLDLLCRSTYCVGNESLGEALITAAALSVLALISDANFL"
     gene            787..1098
                     /locus_tag="VNR65_02030"
     CDS             787..1098
                     /locus_tag="VNR65_02030"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HWJ17483.1"
                     /translation="MADPVVISIPHRLGRAEARRRLEAGIDQVVAPLGRLVSIDRRVW
                     TGDRLDFGMSVAGQTARGLVDVEDAQVRIELELPWVLAAIAKRVRGVIDQRGRGLLEK
                     K"
     gene            complement(1365..>2303)
                     /locus_tag="VNR65_02035"
     CDS             complement(1365..>2303)
                     /locus_tag="VNR65_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWJ17484.1"
                     /translation="GPGGQTAIYAAVRQAGQSVGEAVSPERISRVILMSDGQANVGPS
                     SPAELEQLGRELGSQGIAVTTIGLGLDYSEELMTRMALASDGNHAFVENPEQLADIFN
                     KEFGDVLSVVGQDVDIEILCPDGVTPLRGLGRDVKVDGHRVSFRLNQIGGKQERYLVI
                     ELDIAKGKAQGSAAIADVNVSYLDPKTKARAKVAAQAQVSFSSSSEEATRSANASVAA
                     DVATQLANERSEKAVLARDAGKVADAKQQLEQNAASLRAEASRLQAQSPAAAAPLSKL
                     ADKNEADAKALSSENWNAQRKSMKSDQYRSKTQQSY"
BASE COUNT          426 a          684 c          717 g          476 t
ORIGIN      
        1 gggattgcga gaaaacgggc attaccgctt gcgaccctat gcagaagatg gacgcgatcg
       61 tccaagccga agactcgccc agcttaatcg cgaggctacg attcccgctt gaccccaagg
      121 aagagacatg gcggccaaca agccagaggg cagttctcga cctattgtgt cgatccactt
      181 actgcgtagg gaacgagagc ctcggggagg ctttgattac ggccgcggct ttgagcgttt
      241 tggcgttgat ctctgacgcg aacttcctct gatcccgcgt cagcgcaggc acaagaacga
      301 gagaagcaac gaaaaagaca agcgcaaccg tgacggcgta cttccattca gacagattgg
      361 ccaagatctg aaggagcccg gccccgcggg tgccggttga attgcggctt tctcgcaaga
      421 tagacaacgg gctccaaggc cttgttggtg gagttatcgt catggtactg aacgcttgga
      481 tttgtctctt tgatacgata gcgaacgagt gaggtgcctt agtcttgttg cccgaaggtc
      541 accgttttct tattccgcta tgaactcaag aaaattaact tgactatcct gaacgcgaca
      601 tgaactaacg cgatccgcat tcagcgccta ttcccggtaa aagatcgcgt tcggaaagag
      661 cgccgtgcct cgaacggcaa accggccgct tcaggcgagc gcgcgtcgcg ccggccgcct
      721 tgcaactcaa ggcgccgcgg accacatgat ttttgcggcg agacgctcga acataaaagg
      781 caggtaatgg ccgacccggt cgttatttcc attcctcatc ggcttggcag ggcagaggcg
      841 aggcgtcgtt tggaagcggg catcgatcag gtggtcgcgc cgctcggacg gctggtgtcg
      901 atcgaccgcc gagtgtggac cggcgaccga ctcgactttg gaatgagcgt ggcgggacaa
      961 acagcgcgcg gccttgtgga tgtcgaagac gctcaagtcc ggattgagct tgaactcccc
     1021 tgggttttgg cggccatcgc aaagcgcgtc aggggcgtca ttgatcaacg cgggcgcggt
     1081 cttcttgaaa agaagtgaga ccgactgaaa gtcgcgcgcg ctgacgcggc attcgtgccc
     1141 gtcggcaacg gggagcagta gtaaggcgtg aatgccgctc tatgggaatg gcggctcatc
     1201 ctcctgcaga gatacggcac ccttctcagg gcaggtttcc accgctcgcg aagggcgtcg
     1261 ttcgcaaaat cctcccttca ttctgatcgg cgaagcatcc cgcgtccgag cggacgcggg
     1321 cgtcgttgtg atcgtggtcc caatgtgctt ggcgcaacgc ggcactaata actctgctgc
     1381 gtcttactgc ggtattggtc cgacttcatg gacttgcgtt gcgcattcca attctcggac
     1441 gaaagcgcct tcgcgtcggc ttcgttcttg tccgcgagct tgctcagtgg cgcggctgcg
     1501 gccggcgact gtgcctgaag gcggctggcc tcggcgcgca gggaggccgc attctgctca
     1561 agctgctgct tggcatccgc gaccttgcct gcatcccgcg ccagaacggc cttctcgcta
     1621 cgctcattgg cgagttgcgt cgccacgtct gccgcaacgc ttgcgttggc gctacgcgtc
     1681 gcctcctcgg atgatgagct gaagctgact tgcgcctgcg ctgcgacttt ggcgcgagcc
     1741 ttcgtcttcg ggtcgagata ggagacgttc acatccgcaa ttgcggcgct cccctgagcc
     1801 ttgcctttgg cgatatcgag ttcgatgacg agatagcgct cctgcttgcc gccgatctgg
     1861 ttaagccgga agctgacgcg gtgcccgtcc accttgacat cgcgtccgag cccgcgcaga
     1921 ggcgtcacgc cgtcggggca gaggatctcg atgtcgacgt cctgcccgac aaccgagagc
     1981 acgtcgccaa actctttgtt gaagatgtcc gcgagctgtt cggggttctc gacaaaggcg
     2041 tgattgccgt cgcttgcgag cgccatccgc gtcatcagct cttcgctgta gtcaaggccg
     2101 aggccgatcg tggtgacggc gatgccctgg ctgccgagtt cgcggcctag ctgctcaagc
     2161 tcagcggggg agcttgggcc gacattcgcc tgcccatccg acatgaggat cacccggctg
     2221 atgcgctcgg gcgagaccgc ttccccaacg ctttgcccgg cttggcggac ggcggcgtag
     2281 atcgcagtct ggccgcctgg ccc
//
LOCUS       DATMGX010000123         2771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_274434, whole genome shotgun sequence.
ACCESSION   DATMGX010000123 DATMGX010000000
VERSION     DATMGX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274434"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VNR65_02040"
     CDS             <1..578
                     /locus_tag="VNR65_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ17485.1"
                     /translation="ALTLTVAANSSSAGDWAPFASRNCAWCHGETGQGFTTAPRLAGQ
                     RYEYLITQLRDYNCHKRDNPASQQYMWPAALDINSISGEGLASYFSSIPPVAAKDGNA
                     ELVSTGKNIYTFGIPESNIGSCAACHGPNAEGIRQIPRLGGLGYPYLKERLEQWGEGY
                     HVSAEPPMPQIARRLPPEVIEPLASYLSYVE"
     gene            820..2118
                     /locus_tag="VNR65_02045"
     CDS             820..2118
                     /locus_tag="VNR65_02045"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HWJ17486.1"
                     /translation="MKGTFAAAAAIFLLPASGALAADAAAIDWSKIPAATIPLFYPGQ
                     STYEWLRSPGHQKGQKAVEEGRACFSCHEDDEKDIGKKLVKGGNIEPTPVKDKAGSVD
                     LKVQVAYDDKNAYFRLKWKTQNPYAGDEHRYIRFDGKEWKAYGGPKLDKDVQDGQQPA
                     LSEDRVTMMIDDGKTPKFAQQGCWLTCHNGSPATPNAATSDEIAGNALMKAENQSTVR
                     KYLPVTRNDPGDWKTGKSPEEIAKLKAEGAFLDLIPWYAYRSNPVGMAGDGYVLESRL
                     SDAGKEMFSENEDAATHQPKFMWDEKKAGYKSITEDQLHKAQHVLIKEQNAVAFDPKA
                     GWKAGDMLPAYVLSAADASGSAADDKATGSWKDGEWTVVITRPLALTNDDDKALKAGS
                     TYNVGFAVHDDNVAGRGHFVSLVRSIGFGADGKIKAVKLP"
     gene            2422..>2771
                     /locus_tag="VNR65_02050"
     CDS             2422..>2771
                     /locus_tag="VNR65_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HWJ17487.1"
                     /translation="MAGFGLIAGILFWGGFNWTMELTNTESFCVSCHMMRDNPYKDLK
                     ETIHWSNRTGVRATCPDCHVPKNWFFKIRRKIMATNELYHFALGTVDTPEKFEARKLE
                     LAQRVWATMKSTDSR"
BASE COUNT          666 a          768 c          812 g          525 t
ORIGIN      
        1 cggcgctgac gctcaccgtg gcggcaaaca gttcctcggc gggagattgg gcgccttttg
       61 cctcgagaaa ttgcgcatgg tgccatggcg agaccggcca aggcttcacc accgcgcccc
      121 ggttggccgg gcagaggtac gagtatctta taacccagct ccgggattat aactgccata
      181 agcgcgacaa cccggcttca cagcaatata tgtggccggc ggcgctcgat attaattcca
      241 tatcgggtga agggctggct tcctattttt ccagcattcc acccgtggcc gccaaggacg
      301 gaaacgcaga actcgtatcc acgggaaaaa acatatatac cttcggcatt cccgaatcca
      361 acattgggtc ctgcgccgct tgtcatggcc cgaatgccga ggggatcagg cagatcccgc
      421 gcttaggtgg gctgggctac ccctatctga aagagcggct ggagcaatgg ggcgaagggt
      481 atcatgtctc cgccgagcct ccaatgccac agattgccag gcgcttgccg cctgaggtga
      541 ttgagcctct cgcttcgtat ctaagctacg tcgaataggg ttcacgcgcc cggcgtggcg
      601 cgtgaagcgc aggccgggcg cacttccgac ccgcctgccg cgacgccgta gccttcagac
      661 ctagcctcat ggacctcctt gcacgtcgca ggccagataa ttttgcctcg acaacgctgg
      721 agatttgttg aggataccgt ttcacggcgc tgcaataaac ccgctgatct atgcacgttc
      781 tcgtggcgtg tgttgtattg ctactaagta ggaggaaaaa tgaagggtac gtttgcggct
      841 gcagccgcga tatttttgct gcctgcgagt ggtgccctcg ccgccgatgc agcggcgatt
      901 gactggtcga agattccggc tgcgacaatt ccacttttct acccaggcca gtcgacctat
      961 gaatggctgc gcagtcctgg gcaccagaag gggcaaaaag cagtcgaaga gggcagagct
     1021 tgcttctcct gccacgagga cgacgagaaa gacattggca aaaaactcgt caaaggcggt
     1081 aatatcgaac ccacccctgt caaggacaaa gccggctcgg tcgatctcaa ggttcaggtc
     1141 gcctacgacg acaagaacgc ctacttccgc ttgaagtgga agacgcagaa tccatacgct
     1201 ggagatgagc acaggtacat tcgcttcgac ggcaaggaat ggaaggcgta tggcggccct
     1261 aagctcgaca aggacgttca ggacgggcaa cagccggccc tctctgaaga ccgcgtcacg
     1321 atgatgatcg atgacggtaa gacgccgaag ttcgcccagc agggctgttg gctcacctgt
     1381 cataacggct cgccggccac gcccaatgcc gcaaccagcg acgagattgc tgggaatgcg
     1441 ctcatgaagg cggaaaatca aagtacagtg cgcaaatacc tgccggtgac gcggaacgat
     1501 cccggcgact ggaaaacagg aaagtctccg gaagaaatcg ccaagctcaa agcggaaggg
     1561 gcgttcctgg acttaatccc gtggtacgcc taccgctcga atcctgttgg tatggctggc
     1621 gacggttacg tactggaatc ccgcctcagc gacgctggca aggagatgtt ctcggaaaat
     1681 gaggacgcgg cgactcatca acccaaattc atgtgggacg agaaaaaggc cgggtacaag
     1741 tccattaccg aggaccaact ccacaaggct cagcacgtcc tgatcaagga gcagaatgcc
     1801 gtcgctttcg atccgaaggc cggatggaaa gctggcgaca tgctaccggc ttatgtccta
     1861 agcgcggccg acgcatccgg ctcagcagca gatgataaag cgacggggtc atggaaggac
     1921 ggcgagtgga ccgtcgtcat tacgcggccg cttgccctta caaatgacga cgataaggca
     1981 ttgaaggctg gaagcacata caatgtcggc ttcgccgtgc atgacgacaa tgtcgccgga
     2041 cgtggccact tcgtgtcgct tgtacgctcc atcggctttg gcgcggatgg taagatcaaa
     2101 gcggtgaagc tcccgtgaac ggcgcctcta tctggaattt aggacacccg gcctagggtg
     2161 tggggaccgg cggagctcaa gccggtcctc gcaccaccgg cagctacagt gcgtgcggaa
     2221 aggtgcgaag cggttttccg ataaagacgt acgacaaaac aaaaacctag agcccgcttt
     2281 tgattccacc aaaagcggat aggctctaga ggggcaagaa agagcatgac aatcgatgga
     2341 gaaaaacccg ctaaagagca aactatccta cagaaaagct cgagagtttt ccggccaagt
     2401 gcgcgctact cgatagccgc gttggcgggt ttcgggctga ttgcgggcat cttgttctgg
     2461 gggggattca actggacgat ggaattgacg aacacagagt cattctgtgt ctcatgccac
     2521 atgatgcggg acaaccccta taaggatttg aaggaaacca tccactggtc gaaccgcacg
     2581 ggcgtgcgcg ccacctgccc ggattgccat gtgcccaaga attggttctt caagatccgc
     2641 cgcaagatca tggcaacgaa cgagctctat cactttgctt taggaactgt ggatacgccg
     2701 gagaagttcg aagcccgcaa actcgaattg gcacaacgcg tctgggcaac tatgaaaagc
     2761 acggactcgc g
//
LOCUS       DATMGX010000124         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_461898, whole genome shotgun sequence.
ACCESSION   DATMGX010000124 DATMGX010000000
VERSION     DATMGX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461898"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..822
                     /locus_tag="VNR65_02055"
     CDS             79..822
                     /locus_tag="VNR65_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17488.1"
                     /translation="MALRSKIVSLLAAALSLGVVQAASAADMPTKAPMAAPGVPFSWS
                     GIYFGGSVGGAWGSRDFVYSVGTFPAPNPTDFTASVVGGGFLGAQWQWNSLVLGAEAT
                     FQGMSLKNTETCPNPAFNCVTSANQFWTVGPRVGWSLGQFMIYGTGGYASMKTKYEAD
                     FVAGGATLDSGSSWNHGWFAGGGADWMIPGIPGLILGVDYKHVRANSATVTPTTPAGA
                     PAPGDQYTMSAKADVIQARLSYLWNWTGH"
     gene            complement(1175..1486)
                     /locus_tag="VNR65_02060"
     CDS             complement(1175..1486)
                     /locus_tag="VNR65_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17489.1"
                     /translation="MLVARWQIDARFGHKQTVIDLLKRWERDIGSKAGTDKFDFKLLT
                     GSVGVREATVEADHTVENLAQLERFFEGIGKNEAHKQWGKDLEPYVVSGSSLWTVYRV
                     I"
     gene            complement(1526..1900)
                     /locus_tag="VNR65_02065"
     CDS             complement(1526..1900)
                     /locus_tag="VNR65_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWJ17490.1"
                     /translation="MDAKTASKTNSVTGDALKQAIEARDGRMLSSFYADDATLRIVDR
                     NNPPSKPREVKGRSAITTFWDDICSRAMTHKVETSIENGKQLAFSQACAYPDGMKVLC
                     IALCELKGGKIARQTVVQAWDE"
     gene            complement(1971..>2275)
                     /locus_tag="VNR65_02070"
     CDS             complement(1971..>2275)
                     /locus_tag="VNR65_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWJ17491.1"
                     /translation="EFKSVQLNPKTKLDAKTKELIGLAVAAQIPCQYCIYFHTAVAKA
                     NGATDQEIGEAVAMAAIARHWSTVLNGMQIDLATFKGETDAVLRAAGEKAKTTGMK"
BASE COUNT          383 a          671 c          689 g          532 t
ORIGIN      
        1 gaaaccgggt atccgcctct ttttgccaca gccgcgacag ggtagttttc aaattacggt
       61 tctgtaacgt gtggaggggt ggcgctgcgt tcgaaaattg tgagcttgct tgctgcggcg
      121 ctcagtctcg gtgttgtgca ggccgcttcg gccgccgata tgccgaccaa agcgccgatg
      181 gcggcgccgg gagttccttt cagttggagc ggcatctact tcggtggctc agttggtggg
      241 gcatggggca gtcgtgattt tgtctattcg gtcggcacat ttcctgcccc gaacccgact
      301 gactttaccg cgtccgttgt cggcggcggc tttctcggcg cccagtggca atggaattca
      361 ctcgtgctag gcgccgaagc gaccttccag gggatgtcgc tgaaaaatac cgagacctgt
      421 ccgaatccgg cattcaactg cgtcacgagc gccaaccaat tttggacggt gggtccacgg
      481 gttgggtggt cgcttggcca gttcatgatc tatggcaccg gcggctatgc aagcatgaag
      541 acgaagtatg aagccgattt tgtcgcgggt ggcgctactt tggattccgg aagctcctgg
      601 aaccacggct ggttcgccgg cggcggcgcg gactggatga ttccgggcat ccccggcttg
      661 atcctgggag tcgattacaa gcacgttcgc gccaacagtg cgaccgttac tcccacgacg
      721 ccagccggtg cgccggctcc gggcgaccaa tatacgatga gcgccaaggc cgacgtcatc
      781 caggctcgtt tgagctatct ttggaactgg acgggtcact agtctcacaa acgctgctgc
      841 gtgagccccg ggcatctcgc ccggggcttt tcatttctgc gccgcctcgc ttgccgcaag
      901 tgcggctgaa gccgcgatca aatcgagcgc cgccgcttcg tccggaaacc tcgccgcgcg
      961 cttcgcttag ccgagcgcgg ggcccttctc tacgctgcgc acgctatcga tgaaggatcc
     1021 gctaatacaa cgttttggct catcaatgtc cgttgtgggt cattagcgga cattccggga
     1081 tgccctgcca atggctgctt tacctccaat agcggccaaa tgttgcgggg ccgcgaatgt
     1141 ccgctaaatg ccagaagcag acttaagatt gccgctagat aacgcgataa acagtccaga
     1201 gagacgagcc agagaccaca tagggctcga ggtccttgcc ccactgcttg tgcgcttcat
     1261 ttttaccgat cccctcgaaa aagcgttcga gctgggcgag attttccact gtgtgatcgg
     1321 cttcgactgt tgcctcgcgc acgccgaccg agccggtcaa cagcttgaag tcgaacttgt
     1381 ccgtgccggc cttcgacccg atgtcgcgct cccagcgttt caataggtcg atgaccgttt
     1441 gtttgtgccc gaaacgggca tcgatttgcc atcgtgcaac gagcatgtcg tcctccttta
     1501 aggtgtgatt acgggttgtg acgcgctatt cgtcccaggc ctgcaccact gtctggcgcg
     1561 cgattttgcc gcccttgagt tcgcacagcg cgatgcacag aaccttcatg ccgtccggat
     1621 aggcgcatgc ctggctgaag gcgagctgct tgccgttctc gatactggtc tcgaccttgt
     1681 gcgtcatggc gcggctgcag atatcgtccc agaatgtcgt gattgccgag cggcccttca
     1741 cttcgcgcgg tttgctcggc ggattattgc ggtcgacgat gcgcagcgtg gcgtcatcgg
     1801 catagaagct ggacaacatg cggccgtcac gggcctcgat tgcctgcttg agcgcatcgc
     1861 cggtgactga atttgtctta gaggctgttt tggcgtccat ggtgtcctcc tgatcgtcgg
     1921 gttgggatgc cccgctcccg ttatcgggag cggggcgtaa ggtcgctctg ctatttcatt
     1981 ccagtggtct tggccttctc gccggctgcg cgcagcaccg cgtcggtttc gcccttaaag
     2041 gtggcgagat caatctgcat gccattgagc accgtgctcc agtgccgtgc gatagccgcc
     2101 atcgcgaccg cttcgccgat ttcctggtcg gtcgcgccat tggccttggc cacggcggta
     2161 tggaagtaga tgcaatattg gcacgggatc tgcgccgcca ccgccagccc gatcagctcc
     2221 ttggtcttcg cgtcgagttt ggtcttcgga ttgagctgga ccgatttgaa ctcgg
//
LOCUS       DATMGX010000125         3398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_149494, whole genome shotgun sequence.
ACCESSION   DATMGX010000125 DATMGX010000000
VERSION     DATMGX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149494"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VNR65_02075"
     CDS             <1..192
                     /locus_tag="VNR65_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWJ17492.1"
                     /translation="IREKSRGLTFVLEAIHSAERDVPDYLEVDHNKMTARLARIPQLT
                     DVPYPVVMEPNLVVEFYSR"
     gene            328..666
                     /gene="fdxA"
                     /locus_tag="VNR65_02080"
     CDS             328..666
                     /gene="fdxA"
                     /locus_tag="VNR65_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HWJ17493.1"
                     /translation="MTYVVLDACIKCKFTDCVEVCPVDCFYEGENMLVINPDECIDCG
                     VCEPECPADAIKPDTEPGLENWLELNREYSEKWPNITVKKPHVEDASKYEQETGKFEK
                     YFSANPGSGD"
     gene            complement(960..2060)
                     /gene="mtnA"
                     /locus_tag="VNR65_02085"
     CDS             complement(960..2060)
                     /gene="mtnA"
                     /locus_tag="VNR65_02085"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HWJ17494.1"
                     /translation="MKVGGVSYRTIWDNGDGSVGIIDQTRLPHAFEALTLRTLADAAH
                     AIKIMQVRGAPLIGVTAAYGVCLALNADASDAGLEGACATLAATRPTAVNLAWALREM
                     QKSLSHVPASERVAAAYAKAAQMAEHDVETCRLIGRHGQAIIADIAARKNGPVNVLTH
                     CNAGWLACVDWGTALSPIYHAHDAGIPIHVWVDETRPRNQGAALTAFELGAHGVPHTI
                     IADNAGGHLMQHGAVDLCIVGCDRLTATGDAANKIGTYLKALAAKDNGIPFYVALPQS
                     TIDWTLSDGVLEIPIEERDEREVTHMTGTVASGEILTVQVTAPGSKAANPAFDVTPAR
                     LITGLMTERGVTPASRDGLAQLYPERKETAAA"
     gene            complement(2318..3208)
                     /locus_tag="VNR65_02090"
     CDS             complement(2318..3208)
                     /locus_tag="VNR65_02090"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HWJ17495.1"
                     /translation="MTFTECLDAHASRVERHLDHWLTKERLGAVPARLLEAMRYGSLG
                     GGKRFRPFLVIESAALFGVGAEQALNAACALECVHCYSLIHDDLPAMDDDDLRRGRPT
                     VHKAFDEATAILAGDTLLTLAFEILADPSTHSSPAVRAELGLQLARASGGAGMAGGQV
                     LDLEAPGKTLTEADIRTLQAMKTGALITFGCVAGGILGETSQKNIAALKSFGDALGAA
                     FQLADDLLDAEGDAAVVGKATGKDQEAGKGTLVSLLGIEAAHAKLQEIKAEALRALAP
                     FRPHEGMLAGAAEFVVARKF"
BASE COUNT          647 a         1066 c         1002 g          683 t
ORIGIN      
        1 atccgcgaaa agtctcgtgg gctcaccttc gttctcgaag caatccatag cgcggagcgc
       61 gacgtgcccg attatctcga ggtcgatcat aacaagatga cggcgcggct cgctcgcatt
      121 ccgcagctta ccgacgttcc ttatcccgtc gtgatggagc caaacctcgt cgtcgaattc
      181 tattcacgct aatgctaacg ggcaggcgct ttttccgtgg caatgtgcca tccagatcaa
      241 gcacctgccc gcttgtgaac atcaacggat tgcgttactg acgcgcctat aaattaagag
      301 ccggtgcttc ggcttcggag ataccccatg acctatgtcg tgctggatgc ttgcatcaag
      361 tgcaaattca ccgattgtgt ggaagtctgc cccgtggact gcttctacga gggcgagaac
      421 atgctcgtca tcaatcctga cgagtgcatc gactgcggcg tttgcgagcc ggaatgcccg
      481 gccgacgcca tcaagcctga tactgagccg ggccttgaga attggctgga gcttaaccgc
      541 gaatattcgg agaagtggcc gaacatcacc gtgaagaagc cccacgtcga agacgccagc
      601 aagtacgagc aggaaaccgg caagttcgag aagtacttct cggccaaccc gggctcgggc
      661 gactgagcct tcggtcaacg tcgactattc attcaagggc ttgattggat cgcaaagtga
      721 atctcatgcg gtccagtcgg cattcttgcc ataaggtcgg gctttctcac gatgcttgga
      781 gctgcctgcg cagaggcctt tgacaccaca ccatcagttt gaggatcacc gccagatcgc
      841 ctatggcctt ggccgaggtc agttacacca tcttagagtc ggcatgtaat attggatttg
      901 tgtgcgtagg gccgtaccta gccgcatcta tcaaaaatcc tagtagcacc aatccgttat
      961 caagcggcgg cggtctcttt cctctcagga taaagttgcg ccagcccatc acgagaggcg
     1021 ggagtaacac cccgttccgt catgagcccg gtgatcaggc gcgccggcgt cacgtcgaag
     1081 gccgggtttg cagccttgct gccgggggcg gtcacttgca cggtgaggat ttctccgctg
     1141 gctacggtgc cggtcatatg cgtgacttct ctttcgtcgc gctcctcgat tggaatctcg
     1201 agaacaccat ccgagagcgt ccagtcaatg gtcgactgcg gcagcgcgac gtagaacggt
     1261 atgccattgt ctttcgcggc gagcgccttg aggtaggttc cgatcttgtt tgcggcgtcc
     1321 cccgtcgccg tcaggcggtc gcatccgacg atgcagaggt ccacggcgcc gtgctgcatg
     1381 agatggccgc cggcattatc ggcgatgatt gtgtggggca cgccatgcgc gccaagctcg
     1441 aatgcggtca gcgcggcacc ctggttgcgt ggccgggttt catcgaccca gacatggatc
     1501 ggaattcctg cgtcgtgcgc atgatagatc ggcgagagtg ccgttcccca atcgacgcag
     1561 gcgagccaac ccgcgttgca atgcgtgagc acgttcacag ggccgttctt gcgcgcggcg
     1621 atatccgcga taattgcttg cccatgacgt ccgatcagcc ggcaggtctc gacatcgtgc
     1681 tcggccattt gcgcggcttt cgcataggcc gcagccacac gctcggaagc cgggacgtgg
     1741 ctgagcgact tctgcatctc ccgcagcgcc caggcgagat tcacggccgt gggtctggtc
     1801 gcggccagcg ttgcgcatgc gccttccaac ccggcatcgg atgcgtccgc attcagggcc
     1861 agacaaacac cgtaagcagc cgttacccca atgaggggcg ctccgcgcac ctgcatgatc
     1921 ttaatggcgt gggcagcgtc ggcgagcgtc ctcagcgtaa gcgcttcaaa agcgtgagga
     1981 aggcgcgtct gatcgatgat gccgacggag ccgtcaccat tgtcccagat cgtgcggtaa
     2041 gaaacgccgc caactttcat gtctcactcc agccgtcatc ggccattcaa gagtaatcgg
     2101 atcgccgctt ttcgtgccgt gtcatcgggc agcactcgta cgattatcgg acgcggacgt
     2161 tcccaatgat cgtcgccccg gaatgcgcct cggcgcatat ccggggccgc tacaaatcga
     2221 aacgcctttt gcgatcccgg atacgacgct ggcgtcgttc cgggatgaca gggctttccc
     2281 actgcgtcgc accgagtccg atttcgcacg ctccgcacta gaacttccgg gcgacgacga
     2341 attccgccgc tcccgccagc atgccttcgt gtggccggaa gggcgcgagc gctctcagcg
     2401 cttcggcctt gatctcctgc agcttggcat gcgccgcttc aatccccaga agcgagacca
     2461 gcgtcccctt tccggcttcc tgatccttgc ccgtcgcttt accgacgacg gcggcgtcac
     2521 cctccgcatc aagcagatca tccgcaagct ggaacgctgc gccaagcgcg tcgccgaagc
     2581 tcttcaaagc cgcgatattc ttctgcgacg tctcgccgag aatgccgccc gccacgcagc
     2641 cgaaagtgat gagcgcgccc gtcttcatgg cttgcagcgt gcggatgtcg gcctcggtca
     2701 gcgtcttgcc cggcgcttcg aggtcgagca cctgccctcc tgccatgccc gcgccgccac
     2761 tcgcgcgagc aagctgcagg ccgagctcgg cgcgcacggc ggggctcgag tgcgtgctgg
     2821 ggtctgcaag gatctcgaaa gccaacgtca gcaatgtgtc gcctgcaagg atggcggtcg
     2881 cctcgtcgaa cgccttgtgt acggtcggcc ggccgcgccg cagatcgtcg tcgtccatgg
     2941 cgggcaaatc gtcatgaatg agggagtaac aatgcacgca ctccagcgcg caggctgcat
     3001 tcaaagcctg ctcggcgccg acgccgaaca gggccgcgct ctcgatcacc aggaagggcc
     3061 ggaaccgctt gccgccaccc agcgacccat agcgcatcgc ttccagaagc cgcgcgggaa
     3121 ccgcgccgag gcgctctttc gtcagccaat ggtcgagatg gcgttcaacg cggcttgcat
     3181 gggcgtccag gcattctgta aaggtcatgg cgaaactaca tttcttattg gccgagcccc
     3241 ggcaacatgg accagataag ttccatgctc aagcgtgttt tgtgagctgc gtcatgacgg
     3301 atatcgcacc gcaagttctg cagccggacg gcattccctc cggcgagcaa aaagaaaaag
     3361 gctcaccccg acggcggatc tttcgcgcgg ggcttatt
//
LOCUS       DATMGX010000126         2101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_149471, whole genome shotgun sequence.
ACCESSION   DATMGX010000126 DATMGX010000000
VERSION     DATMGX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149471"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /gene="nadC"
                     /locus_tag="VNR65_02095"
     CDS             <1..694
                     /gene="nadC"
                     /locus_tag="VNR65_02095"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWJ17496.1"
                     /translation="GMPLVAAAFRALDPEIIIEPHVKEGDVALAGSVVARIRGRTRAI
                     LTAERVALNLLQHLSGIATATRHFVEAVAGTHTRIVCTRKTVPGLRALEKYAVRAGGG
                     FNHRFGLFDGVLIKDNHIAAAGSVSAAIERVLAANGHLVKVEVEVDTLVQLEEALQHP
                     IDAVLLDNMAVPELKNAVARAKMVKPAILCEASGGVRLVTVRSIAETGVDMISVGALT
                     HSVKSLDIGLDF"
     gene            complement(1261..1887)
                     /locus_tag="VNR65_02100"
     CDS             complement(1261..1887)
                     /locus_tag="VNR65_02100"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HWJ17497.1"
                     /translation="MRVFQIIANAQSAHIFSNMGEAFGFSDELMAQTVRYFVPPLTKS
                     IEKRAQTPEGLISVLEFLGSRRYDRFLDDPRIFGHAQISDEGQRILDYLFVRKERVQK
                     IIENRAKVLPLDPVTLEKLFPFIAVLAIAAIELRTRRPLGLILYRVTKGATDDRAIAN
                     PFLALVQYLRKQEQQQKDQRKRRLFSFFGSSEQPAQKTEPAAGFFQSA"
BASE COUNT          445 a          585 c          603 g          468 t
ORIGIN      
        1 cgggatgcct ctggtagccg ccgctttccg agcccttgac ccggaaatca ttatcgagcc
       61 ccatgtgaag gaaggggacg tcgctttggc cgggagcgtc gtcgcccgca tcagaggaag
      121 gacgcgcgct atcctcacgg ccgaacgcgt cgccctcaat ctcctgcaac acctctccgg
      181 catcgccaca gccacgcgcc atttcgtcga ggccgtcgcc ggcacccata cgcgcatcgt
      241 gtgcacgcgc aagacggtgc cgggccttcg cgcgctagag aaatacgccg tgcgggcggg
      301 cggcgggttc aatcaccggt tcggtctttt tgacggggtc ctcatcaagg acaatcacat
      361 cgctgcggcc gggagcgttt cagcggcaat cgaaagggtt ctggcggcaa acggccatct
      421 ggtgaaggtc gaggtcgagg tcgatacgct ggttcagttg gaagaggcgc ttcagcatcc
      481 catcgacgcg gttctgctcg acaatatggc cgtgccggaa ctgaaaaatg ccgtagcgcg
      541 agcgaagatg gtaaagcctg cgattttgtg tgaggcttcc gggggcgtgc gccttgtgac
      601 ggtgcgttcg atcgccgaaa cgggcgtcga tatgatttcc gtcggggcgc tcacccattc
      661 cgtcaaaagc ctggatatag ggctcgactt ctagatacgt cgctcttttg cccaggctgt
      721 ttgccttatc cccactggga ataggccgct cgagttgctg aggttgtagc gtattcggac
      781 gacccgttta tctccaaggc actcagaaga gcaaggaagt cattctgcgc tcggctggtc
      841 gtatccgctg tgttcgcgct atttgatgtg gattttgtct gcgtttgagc cgccgaattc
      901 gaatccgatg tttggttgtt ggcttcttgc ccgtgacgat gatggtagtg ggcgcccccc
      961 accgccgcac cggtgcaaat atccgaaatt gacatggtca tctcccaaaa agcttgtagt
     1021 tcctccgcat agagcgagag gcttcgggaa cagacgccgc ttttccagga ctacactcgc
     1081 ttcagaagat cctgcaaaac aattcaatct tagattgcat tgaatcgatc ttcaagcata
     1141 atagtaaaga tattaacagt tcggagggcg ccgttggttc gaaagcgccc accacgcagc
     1201 accctcaact ccgtttcgag ccggagaagg gcaaccgtca gtaaagccgt gaaaacgtcc
     1261 tcaggctgac tggaagaacc cggctgccgg ttcggttttt tgagcgggct gctccgagct
     1321 gccgaagaag gagaacagcc gcctcttgcg ctgatccttc tgttgctgct cctgcttcct
     1381 gagatattgc accagggcaa gaaacggatt cgcaatcgca cggtcgtccg ttgcgccctt
     1441 cgtgacgcga tacaggatca agccgagcgg acggcgcgtc ctcagttcga ttgccgcgat
     1501 agccagcacg gcgataaagg gaaacagttt ttcgagcgtg acgggatcga ggggcaagac
     1561 cttggcgcgg ttctcgatga ttttctgaac gcgttccttg cgcacaaaga gataatcgag
     1621 gatgcgctgc ccttcgtcgg agatctgggc atgaccgaag atccgtgggt cgtccaggaa
     1681 gcggtcgtag cggcgtgagc caagaaattc gagcaccgaa atcaagcctt caggcgtttg
     1741 agcgcgtttc tcgatgcttt ttgttaaggg gggaacgaaa tatcgaacgg tctgcgccat
     1801 cagttcgtcg ctgaacccga acgcttcccc catgtttgaa aatatatgcg cactctgcgc
     1861 gttagcgata atttgaaaaa ctctcatggc tccgcctcgc tcgcccacct tggcccgaag
     1921 ctttcgcccc taagcctttg cccctgggaa actcgatgta cgcaacgacg ccagcgattc
     1981 tgaacacctg aggtgcagtc gaatcataac tctaataaag tgtaaacaga actggggtat
     2041 cggcgaagac aagtaaacct cgcctgccgt cagaatcagc tttattgatc actgttgtgt
     2101 t
//
LOCUS       DATMGX010000127         3299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_217070, whole genome shotgun sequence.
ACCESSION   DATMGX010000127 DATMGX010000000
VERSION     DATMGX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217070"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            448..1428
                     /gene="recA"
                     /locus_tag="VNR65_02105"
                     /pseudo
     CDS             448..1428
                     /gene="recA"
                     /locus_tag="VNR65_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
     gene            1724..>3299
                     /gene="alaS"
                     /locus_tag="VNR65_02110"
     CDS             1724..>3299
                     /gene="alaS"
                     /locus_tag="VNR65_02110"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWJ17498.1"
                     /translation="MSTLNDIRGTFVDFFRKNGHEHVPSSPLVPRNDPTLMFTNAGMV
                     QFKNIFTGMETLPFKRAVTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KDVAIELAWNLVTKEYGLDKKRLLVTVFSEDDEAFGLWRKIAGLPEGRIIRIPTSDNF
                     WSMGETGPCGPCSEIFYDHGAEIPGGPPGSEDADGDRFVEIWNLVFMQYEQISKSERQ
                     PLPKPSIDTGMGLERIAAVLQGVHNNYDTDLFQAIIRASVDATGVAAEGSALPSHRVI
                     ADHLRASSFLIADGVLPSNEGRGYVLRRIMRRAMRHAHILGAEEPLLYRLLPVLIHEM
                     GQAYPELHRGQSLIGETLKIEEIRFHRTLARGLTLLDEASGALKPGDTLSGDVAFKLY
                     DTYGFPLDLTQDALKARDVGVDVEQFNAAMAQQKAEARKAWAGSGEAATDKLWFDLLD
                     QVGPTEFLGYATETAEGEVLALIKDGNLVKQLKKGEDGIVILNQTPFYGESGGQVGDH
                     GVIMAPKGVHFEVTETQ"
BASE COUNT          754 a          947 c          941 g          657 t
ORIGIN      
        1 cggctccgcg gcgctcatct ggccgagcga catttcagcc ggactcgcgg gcgcggtcag
       61 cggcgccgcc actgccaact gctgaaaacc gaaaagcatc gacgctccca acgccaagcc
      121 aagaacctta tttttcatat acgcctccct tgagaaaact ttaccgagat cgacaacctg
      181 agccgtaaca atgtttgccc gaagtgcttt gattacaagg tggcgttcat ttgcgatcga
      241 acttttgggc cttggccgtt cacgaggcga tgcgatcgct cgttttctgc aaagcgaagt
      301 acagtgggcc ggattttcca cttgcacaca agaacaaaac gtgtacattc ggtgtcgttt
      361 gcaagtccat catccctatg aaataacaaa gggcaacgat tcatgaatga tacgccattc
      421 cgcgtgatcg aagggaatct tactatcatg gacaagcaga aagccctcga agcggcgctg
      481 agccaaatcg agcggaattt cggtaaaggc tccatcatgc ggcttggcaa gaacgaaaag
      541 cccgtggagg tggaagccat ttctacaggt tcattgggcc tagacatcgc tctcggcatc
      601 ggcggcctgc cacgcggacg cgtcattgaa atatttggcc ctgaatcatc cggcaagacg
      661 acgcttgccc tccatgccat cgccgaggcg cagaagaaag gcggcgcttg cggttttgtg
      721 gacgcggagc acgcgctcga tcccgtttat gcgagaaagc tcggggtcaa cctcgaagag
      781 cttatcattt cacagccgga tacaggcgag caggcgcttg aaatcaccga tacgctggtc
      841 cgttccggag caatcgacgt gattgtggtc gactcagtcg ccgcgctcac gcctaaggca
      901 gagatcgagg gcgagatggg cgatgtgctg ccgggcatgc aagcccgcct catgagccaa
      961 gcgcttcgca agctcacagg ctccatctca aaatcgaaca ccctcgttat attcatcaat
     1021 cagatccgca tgaagatcgg cgtgatgttc ggcaatccgg agacgacgac gggtggaaac
     1081 gcgctcaagt tttattcttc cgtgcggctc gacatccggc gtattggaca gatcaaggaa
     1141 cgcgatgagg ttgtcggcaa ccagacacgc gtcaaggtcg ttaagaacaa ggttgcgccg
     1201 ccgttcaagc aggtcgaatt cgacatcatg tatggcgagg gcatctcaaa gacgggcgaa
     1261 ttggtcgacc ttggcgtaaa agccaacatc gtcgagaaat caggctcttg gtactcccat
     1321 gacagccagc gcatcggaca gggtcgcgag aatgccaagc tctacctgaa gaacaacccg
     1381 caagttgccg aagccatcga gcgcgaaatc agggcgaatg ccgggctgat cgcatcgcga
     1441 atccttgacg gaaccgacgc tgacgagact gtaggcgaag gggacatagc ggaatccgaa
     1501 gaggctgtgc cggcgcgcaa aggcggccgc cgataactgt caggaaataa accaagtgaa
     1561 acttttcctg gggcaaggcg cccgcttgat tgctgtgggt tgattgctcc gtaacgagaa
     1621 tttgctatat cgctcctcac agccaatatg ctttatctct ggcgcgggca cgttcctcac
     1681 ccacattgaa caccgcgcta gcccactgga aatgaaattc gttatgagca ccctcaacga
     1741 tatacgcggg actttcgtcg attttttccg gaagaacggc cacgaacatg tgccgtcgag
     1801 cccgcttgtg ccgcgcaacg atcccacgtt gatgtttacc aacgccggca tggtgcagtt
     1861 caagaacatc ttcaccggga tggagacact gccattcaag cgcgcggtga catcccagaa
     1921 gtgcgtgcgc gcggggggca agcataacga tctcgacaat gtcgggtaca cggctcgcca
     1981 tcacaccttc tttgaaatgc tcggcaattt ttcttttggc gactatttca aggatgtggc
     2041 catagagctt gcctggaacc ttgtcaccaa agagtacggc ctcgataaga aacgcctcct
     2101 cgtcaccgtc ttttccgaag atgacgaggc tttcgggctc tggcgcaaaa tcgcggggct
     2161 gcccgaaggc aggatcatac gcatcccgac ctcggacaat ttctggtcga tgggagagac
     2221 cggtccttgc ggcccatgct ccgaaatctt ctatgatcat ggggctgaaa tccctggcgg
     2281 ccctccaggc agcgaagatg cagatggcga ccgtttcgtc gaaatctgga acctcgtgtt
     2341 catgcaatac gagcagattt ccaagagtga acgtcaaccc ctgccgaaac cttcgatcga
     2401 taccggcatg gggcttgagc gtatcgctgc cgtgctccaa ggcgtccaca acaactacga
     2461 cacagacctt ttccaggcga tcataagggc atccgtcgat gccactggcg tcgccgccga
     2521 aggctctgcg ctcccgagcc accgcgtgat cgcagatcac ctcagggcgt cgagctttct
     2581 catcgcggat ggcgtgctgc cctcgaatga ggggcgcggc tacgttctgc gccgcatcat
     2641 gcgccgcgcc atgcgccatg cgcatatcct gggtgcagag gagccgctgc tttatcgcct
     2701 tctgccggtc ctcatccacg agatgggaca agcctacccg gaacttcacc gcggccagtc
     2761 gctcatcggc gagacattga agattgagga aatccgtttc cacagaacgc tagcgcgggg
     2821 cctgaccctt ctcgacgagg cttccggcgc gctgaagccg ggcgacacgt tgtcgggcga
     2881 tgtcgccttc aagctctacg atacctatgg tttcccgctc gaccttacgc aggacgcttt
     2941 gaaggcgcgc gacgtcgggg tcgatgtcga gcagttcaac gcggcgatgg ctcagcagaa
     3001 ggcggaggcg cgcaaagcgt gggcaggctc gggcgaagca gccacggaca agctttggtt
     3061 cgatcttttg gatcaggtcg gccccaccga gtttctgggc tacgcgaccg agaccgccga
     3121 aggcgaagtc ttggcgctca tcaaggacgg caatctcgta aagcagctca aaaagggcga
     3181 ggacggcatc gtcatcctca atcagacgcc cttttatggc gaatccggcg gccaggtcgg
     3241 cgatcacggc gtgatcatgg ctccgaaggg cgtgcatttc gaggtcacgg aaacgcaga
//
LOCUS       DATMGX010000128         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_375217, whole genome shotgun sequence.
ACCESSION   DATMGX010000128 DATMGX010000000
VERSION     DATMGX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375217"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1487)
                     /locus_tag="VNR65_02115"
     CDS             complement(180..1487)
                     /locus_tag="VNR65_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17499.1"
                     /translation="MIPHSQTDAKLQQSSPPLLSENEPHLAHLLEHAIRGSGLHERAQ
                     FEAGTADSSTQAADPGFLHGRLIEELTTAQEHMRAETPAQYAEQPLIYAEPAAQDEAE
                     PPVHYGKPLDYADPAVEYSVFEEPQYTTFELTDQEVASRPDLISSALPEDTVSREPDV
                     EPRRVTDALEEHLSADTLRRSSGDVAGADRTNTSGQKARSLTRRIRLRDWKRRRLALL
                     SHIHRHVFDRKTEQLLFCKTPLLEVYHAEDTEKGLEKSFFYQGPIPKKLLDWALSALP
                     HDLKRHAFVDFRAGNGRTLLLAARHNFEYAAGYAFDMEGSETLEMNLAQYPRTYLACR
                     DVRALRGDRDGVLIPSQPSVLFFPDSLSAGHLDVILTYVTTSLRLDPRPIYLIFENAG
                     RERSKEQMSLFKKVPLPLLNQAKAFLFAPVSIAVYRSKIETAN"
     gene            1620..>2068
                     /locus_tag="VNR65_02120"
     CDS             1620..>2068
                     /locus_tag="VNR65_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17500.1"
                     /translation="MIFHNFAWIGNQAGASSLRNTVTTPIPLFLVAILLRVNLSLSKS
                     WLLVVILMCKESPRTMASFGQIRSRPRDAFQSILVRLYKFALVLTVNEELARALLRST
                     FKGQNLRKDFSGDERDHLIEAFRRMYALWNANPSIQKQCQPEPRLFAA"
BASE COUNT          433 a          563 c          599 g          473 t
ORIGIN      
        1 agatatgcgg gttcttcgcg catgcatgcg tgaacgcctc gagccccctg atcatcccga
       61 gggtgagcgc attcaaggct ttcggccggt taaggcggat gagaccggcg cggcctcgtt
      121 cggcgcaaag gagttcgttc ggcgcttcgt ccatgttttc cgtcgacgac aagagttggc
      181 tagttcgctg tttcaatctt ggagcgataa acggcaatgc tcacgggcgc gaataggaac
      241 gccttggcct gattgagcaa aggcagcggt actttcttaa acaggctcat ctgttccttg
      301 ctgcgttcgc gtcccgcatt ctcgaagatg agataaattg ggcgcggatc gagccggaga
      361 gaggtggtta cataggtcag gatgacgtcg aggtggcctg cgctgaggct gtccgggaaa
      421 aagagaacgg aaggctggga aggaatgagg accccatcgc gatctcctcg cagcgcccgg
      481 acatcccggc acgccagata ggttctcgga tattgggcca gattcatctc aagcgtttcc
      541 gagccttcca tatcgaaagc gtatccggcc gcatattcga aattatgccg ggcagccaga
      601 agaagagtgc gcccattgcc cgcacgaaaa tcgacgaaag catggcgctt caaatcatgg
      661 ggtagcgccg aaagcgccca gtcgagaagc tttttaggga tcggtccttg atagaagaaa
      721 gacttctcca gacccttttc tgtgtcttcc gcatgataga cttcaagcaa aggggttttg
      781 cagaaaagaa gctgctctgt ctttctgtcg aagacgtgac gatgaatatg gcttaacagt
      841 gcgaggcggc ggcgtttcca atcccgcagc cttattcggc gcgttaggct tcgagccttc
      901 tgaccggagg tgttcgtcct gtcagcgcca gcaacgtctc cggaactcct ccgcagcgtg
      961 tccgcgctca agtgctcttc caaagcgtcg gtaacccttc tgggctccac atccggttcc
     1021 ctggaaactg tatcctcggg cagtgctgac gagatcagat ctggacggga tgccacttcc
     1081 tgatcggtga gttcaaaggt tgtgtattga ggctcttcaa acaccgaata ctcaacagca
     1141 gggtccgcat agtccagcgg cttcccatag tgaacaggcg gttcggcctc gtcctgcgcg
     1201 gccggctccg catatatcag gggctgctcc gcgtactggg ccggcgtttc ggcccgcatg
     1261 tgctcctgtg cagtcgtcag ttcctcgatc agccttccat gcagaaaacc agggtcggcc
     1321 gcctgcgtcg aggaatcggc cgtaccggcc tcaaactggg cgcgctcatg aaggccgcta
     1381 ccgcggatcg catgttccag gagatgagcg aggtggggct cgttctccga aaggagcgga
     1441 ggagacgact gttgcagctt ggcatcagtt tgggaatgtg ggatcaaacg ccgatcaacg
     1501 ttggagaatt tactgagccg gttttctgcc gaatcgtcac tcatcgtggg gcggtccgca
     1561 cctgacgtcg ccggaacaga gagccatcgc tgccggcttt aagaaaaaat tcacggactg
     1621 tgatttttca caactttgcg tggattggca atcaggcagg cgcctcatcg ttaaggaaca
     1681 cggtgacaac cccaattccg ctatttcttg ttgcaatttt actgcgcgtc aacctttccc
     1741 tgtcaaagtc ttggctgttg gtcgttattt taatgtgtaa ggagagtccg cgtaccatgg
     1801 cttcctttgg acagatccgc agccgcccgc gcgatgcctt ccagagcatc cttgtccgcc
     1861 tatataaatt cgctctcgtg ttgacggtca atgaagaact ggcgcgggcg cttctgcgca
     1921 gcacgttcaa gggtcagaat ttgcgcaagg atttttctgg ggatgagcgc gaccatctca
     1981 tcgaagcgtt ccggcgcatg tatgctttgt ggaatgcgaa ccccagcatc caaaagcaat
     2041 gtcagcccga gccacgcctg tttgcagc
//
LOCUS       DATMGX010000129         3027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_15797, whole genome shotgun sequence.
ACCESSION   DATMGX010000129 DATMGX010000000
VERSION     DATMGX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15797"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VNR65_02125"
     CDS             <1..973
                     /locus_tag="VNR65_02125"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWJ17501.1"
                     /translation="MEAILRVYNLHGRRDNIYKARIKILVHDLGAQRFAAEVEEEWSR
                     IKDGYMKLDPAFVGDIRSRFIYPQYEDLDDGPSELRRALSADPKFALWYENSVSRHKV
                     PGYSIVTLSLKPPGKPPGDATSGQMEAIADLADKYSHGEIRVGHEQNLALPHVRQEDL
                     PELWQKLDALGVATPNVNLVTDIISCPGLDYCSLANARAIPIAMEISRRFEEIDKARS
                     VGRLHVNISGCINACGHHHVGHIGLLGVEKNDKEFYQITLGGKADEDARLGQLLGPAI
                     PYEEVTDTVERVVNRYLELRLHEGELFIDTVKRLGIAPFKEALHAVH"
     gene            960..1481
                     /locus_tag="VNR65_02130"
     CDS             960..1481
                     /locus_tag="VNR65_02130"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HWJ17502.1"
                     /translation="MRYIKNSAVAADPFVYIEGDEPVPSDVPAIVSADWLLSAAKQQI
                     QDRSAPLGVNWPNDKPESELAPFLTDLSLIALEFPVFRDGRAFTQARLLRERHGFKGE
                     IRATGDVLRDQFLFMVRAGFDAFEVKKPADADAFAKAVHEFTLRYQPAADGPLHAFRS
                     RLDLPEKGKQGDA"
     gene            1586..2578
                     /locus_tag="VNR65_02135"
     CDS             1586..2578
                     /locus_tag="VNR65_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWJ17503.1"
                     /translation="MLRRIVLIALSAAVLALPVRAADKTLLNVSYDPTRELYVEVNKA
                     FSSKWKADKSDALTIKASHGGSGKQARAVIDGLEADVVTLALAYDIDAIADKGLLAKD
                     WQKKFPDNASPYTSTIVFLVRKGNPKGIKDWDDIVKPGVQIITPNPKTSGGARWNYLA
                     AWGYALKKYGSEDKAKEFIEKLYKQVPVLDTGARGATVTFVERGQGDVLLAWENEAYL
                     SVKEFGADKFEIVTPSTSILAEPPVAIVDKVVDKRGTRAVAEAYLNFLYTKEGQEIAV
                     ANFYRPRLKEVADKHADQFSKLDLFTIDDVFGGWQKAQKTHFGDGGIFDQIYGK"
     gene            2593..>3027
                     /locus_tag="VNR65_02140"
     CDS             2593..>3027
                     /locus_tag="VNR65_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17504.1"
                     /translation="MKTFTYNGTGENLTGNMRSGPLGHGNAFPRFPESTVIPGFGLTF
                     GLTLTWLAVIVLIPISALLLKASSISAQEFLNILLAPRTLNALKLSFGLSFVAAIINV
                     FFGLIVAWALTRYEFPGRRLADAIVDIPFALPTAVAGIALASL"
BASE COUNT          651 a          882 c          872 g          622 t
ORIGIN      
        1 catggaagcc attttgcggg tctacaattt gcatggccgc cgggacaaca tctacaaggc
       61 ccggatcaaa attctggtgc atgaccttgg cgctcaacgc ttcgccgcgg aagtcgagga
      121 ggagtggtcg cgtatcaaag atggctatat gaagctcgat cctgctttcg taggcgatat
      181 ccgctcgcgc ttcatctatc cccaatatga agacctcgat gacggcccta gcgagctgag
      241 acgcgcgctc tcggccgatc cgaaattcgc gctgtggtac gaaaactcgg tttcgcgcca
      301 caaagtaccc ggctattcga tcgtcacact gtcgctcaag ccgcccggaa agccccccgg
      361 agatgcgacg tcgggccaga tggaagccat cgccgatctc gctgacaagt atagccacgg
      421 cgagatccgt gtcgggcatg agcaaaacct ggcgctgccg catgtccgcc aggaggactt
      481 gcccgagctg tggcagaagc tcgatgcact cggcgtggca acaccgaacg tcaacctcgt
      541 gacggacatc atctcctgcc ccggcctcga ttattgcagc cttgcgaatg cgcgcgccat
      601 ccccattgcc atggagatct cacgccgctt cgaagagatt gacaaagctc ggtctgtggg
      661 acggctgcac gtcaacattt cgggctgcat caacgcctgc ggccatcatc acgtgggcca
      721 tatcggcctc ctcggagtcg agaaaaacga caaggagttc tatcagatca cgctgggcgg
      781 aaaggccgac gaagacgcaa ggctgggtca gctcttaggc ccggcaattc catatgagga
      841 agtgaccgat accgtagagc gagtagtgaa tcgctatctt gagctccggc ttcatgaggg
      901 tgagctattc atcgacaccg tgaaacgtct cggcatagcc ccgttcaagg aggcgctcca
      961 tgcggtacat taagaacagc gccgtcgctg ccgacccctt cgtttacatc gagggtgatg
     1021 aaccagtccc ctcggatgtc cctgcgatcg tgtcggcgga ctggcttctc tccgccgcga
     1081 agcagcagat tcaggatcgg agcgcacctt tgggtgtgaa ctggcccaac gacaagcccg
     1141 aaagcgagct tgcgcctttt cttacggacc tgtcgctgat tgcactcgaa ttccccgtct
     1201 tccgcgatgg ccgcgccttc acacaagccc gcctgcttcg cgagcggcat ggtttcaaag
     1261 gtgagatcag ggccacgggc gacgtgcttc gcgatcagtt tctgttcatg gtccgggccg
     1321 ggttcgacgc cttcgaggtg aagaagcctg cagacgccga cgctttcgcg aaagccgttc
     1381 atgagttcac gcttcgttac cagcccgctg cagatggccc actgcacgct tttcgttccc
     1441 ggctggacct gccggaaaag gggaagcaag gcgatgcata aggtccttgt cataaaccat
     1501 tcgcactgtc ggaaccctca ttgaccggcg ctggccgcga tcaagggggg catcgagtgt
     1561 ttcattcaga atctggagat tttttatgct gagacggatt gtcttgatag cgttatcggc
     1621 tgcggttcta gccctgcctg tccgcgccgc cgacaagaca ttgctgaatg tgtcctacga
     1681 tccaacccgc gagctttacg tcgaggtcaa caaagccttt tccagcaaat ggaaagctga
     1741 caaaagcgat gcgctcacga tcaaggcgag ccacgggggg tccgggaaac aggcgcgagc
     1801 cgttatcgac gggctcgaag ccgatgtcgt gactctggcg ctcgcctatg acatcgacgc
     1861 catcgcggat aaggggcttc ttgcaaaaga ttggcagaag aaatttccgg ataacgcttc
     1921 accctacacc tcgacgattg tctttctcgt ccgaaaaggc aatccgaaag gcatcaagga
     1981 ctgggatgat attgtaaagc ccggcgtcca gatcatcacg ccgaatccga aaacatccgg
     2041 cggcgcgcgg tggaattatc tggcggcatg gggatacgct ctcaagaaat acggctccga
     2101 ggataaggcg aaggagttca tcgagaagct ttacaagcag gtgcccgttc tcgataccgg
     2161 cgcgcgcggc gccaccgtca ccttcgtcga gcgtggccaa ggcgatgttc tcctggcctg
     2221 ggagaacgaa gcctacctct ctgtgaaaga gttcggcgcg gacaagttcg agatcgtaac
     2281 gccgtccacc tccatcctcg ccgagccgcc tgtggcaatc gtggacaagg ttgtcgacaa
     2341 gcgcggcacg agggccgtcg cggaggcata cttgaacttc ctctacacca aagaaggcca
     2401 ggagatcgcg gtagcgaatt tctatcgtcc gcgcctgaaa gaggtcgcgg acaagcatgc
     2461 cgaccagttt tcaaagctgg atctgttcac catcgatgat gtcttcggcg gctggcaaaa
     2521 ggcccagaaa acgcacttcg gggacggcgg cattttcgac caaatttacg gaaaatgaca
     2581 agtggcacaa aggtgaagac attcacatat aatggtacgg gggaaaacct gacgggcaat
     2641 atgaggagtg ggcctttggg tcacggcaat gccttccccc gttttccgga gtcgacagtc
     2701 ataccgggct tcgggctgac ctttggcctc acgctgacat ggcttgcggt catcgtgctc
     2761 attccgatct ccgcgctgct gctaaaggca tcctcgatca gcgctcagga gtttctgaat
     2821 atcctcctcg caccacgcac gctgaatgcg ctgaagctca gcttcgggct ttccttcgtg
     2881 gccgcgatca tcaacgtgtt cttcgggttg atcgtagcct gggcgctcac ccggtatgaa
     2941 tttccgggca ggcgcctggc agacgcgatt gtcgatatcc cgttcgcact gccgacagcc
     3001 gtggcgggca tcgcgttggc gagcctt
//
LOCUS       DATMGX010000130         3332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_443080, whole genome shotgun sequence.
ACCESSION   DATMGX010000130 DATMGX010000000
VERSION     DATMGX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443080"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..2691)
                     /gene="pepN"
                     /locus_tag="VNR65_02145"
     CDS             complement(25..2691)
                     /gene="pepN"
                     /locus_tag="VNR65_02145"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HWJ17505.1"
                     /translation="MKTDLPRTIYLKDYTPPPFLISKTELDVALSPSQTSVKARLSVH
                     PNPKVKGKTHPLTLDGELLQLMRVTIDGQDLPASRYVLTEKDLTIETVPSAPFTLEIE
                     TICDPEANKALSGLYRSQGVYCTQCEAEGFRRITYYLDRPDVLSKFRVRIEADRKDAP
                     ILLSNGNLIESGEAGPGRHFAVWEDPFPKPSYLFALVAGKLGMVQDSFTTRSGRKVDL
                     RIYVEPGKDDRCAWAMESLKRSMKWDEERFGLEYDLDTFMIVAVSDFNMGAMENKGLN
                     VFNDALILARPETATDADYESIEAVIAHEYFHNWTGNRVTCRDWFQLCLKEGLTVFRD
                     QEFSADVRSAVVKRIQEVRFLKSAQFPEDAGPLAHPARPSSFIEINNFYTRTVYNKGA
                     ELCRMLQTVLGKHGFRRGLDLYFKRHDGQAVTVEDFVSALGDANKTDLSEFMLWYNQA
                     GTPSLDARLTYSQGTKSARLTLSQSYPPVPEGAKRKPVPIPVRLGLVGGNGEDLPLKE
                     NGKPVPDGVVMLRKKKEVFTFEDVADRPVLSILRDFSAPVKLYTSASDREMLTLIRSD
                     SDLFNRWQTAQAFALKHIADMAKAIASGSEPRVNPRFVQAAGAVANDDSLEHAYRATF
                     LALPSESDVAQYIGENVDPEAIHIARNTLRSAFGRSLRTVLEGIYEASAPAEPYRPDA
                     ESVGKRALRQACLILLAAAKSRSGIAKVKEQARTATNMTEAMGALSILSLVGGEAYDQ
                     ALQRFYRRWKDEALVMNKWFALQAISPSPDTLKRVQELTAHPQFSLKNPNRVRSVYGA
                     FAHGNQVRFNDAGGEGYELIASAVIDIDGFNPQIASRLVGAFESWRIFEPKRRALALA
                     ALKRVLAKKSLSSDVFEIASKIVGEEAGAAAA"
     gene            2872..>3332
                     /gene="ruvC"
                     /locus_tag="VNR65_02150"
     CDS             2872..>3332
                     /gene="ruvC"
                     /locus_tag="VNR65_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWJ17506.1"
                     /translation="MTATGDTEDLGASEFGTGRLMEKKNTRILGLDPGLCNLGWGVVD
                     WDGARLSFVGCGTIKTDADEPMGQRLATLFAGLEEVVQRWLPMEAAVEETFVNGNARS
                     ALKLGQARGVALLVPARLGLDVAEYAPNTVKKTVTGSGHADKAQIRAMIGY"
BASE COUNT          644 a          970 c         1029 g          689 t
ORIGIN      
        1 ggttgaaatt gccgtaattt ttgttcaagc agccgccgct ccggcctcct cgccaacgat
       61 ctttgatgcg atctcgaaca cgtccgagga gaggctcttc ttcgccagca cccgtttgag
      121 agccgccaaa gcaagcgcac gccgcttcgg ctcgaagatg cgccagctct cgaaagcgcc
      181 gacgagtctc gatgcgattt gcgggttgaa gccgtcgatg tcgataacgg cgctcgcgat
      241 gagttcatag ccctcgccgc ccgcatcgtt gaagcgaacc tgattgccgt gcgcaaacgc
      301 accgtagacg gagcggaccc tgttcgggtt cttgagcgag aactgcggat gcgcggtgag
      361 ttcctgcacc cgcttcagcg tatccgggct cggcgagatt gcctgcaagg cgaaccactt
      421 gttcatcaca agggcttcat ccttccagcg ccgatagaag cgttgaagcg cctgatcgta
      481 ggcttctccg ccgacgagcg acaggatcga caacgccccc atggcctcgg tcatgttcgt
      541 tgccgtgcgg gcctgctcct tgaccttagc gatgccggag cggctcttgg cggccgcaag
      601 caggattaag caagcctggc gaagcgcacg cttgcccacg ctttccgcat cgggacgata
      661 aggttctgcg ggcgcgcttg cctcgtaaat cccttcgagg acggtccgaa ggctgcggcc
      721 gaaagccgag cgcaaagtgt tacgcgcgat atggatcgct tcggggtcga cattctcccc
      781 gatatactgc gcgacgtcgg actctgacgg aagcgccagg aacgtggctc tataggcgtg
      841 ctccaggctg tcgtcgttcg caacggcgcc cgcggcttgc acgaagcgcg gattgacacg
      901 cggctctgac ccgcttgcta ttgctttcgc catgtctgcg atatgcttga gcgcaaacgc
      961 ttgcgctgtt tgccagcgat tgaagaggtc gctgtccgag cggatgagcg tcagcatctc
     1021 gcgatcgctg gccgaggtgt agagcttaac gggcgcagaa aaatcgcgaa ggatggacag
     1081 cacgggccga tcggcgacat cttcgaaggt gaagacttct ttcttcttcc gaagcatgac
     1141 cacgccatca ggcaccggct tgccattctc tttcagcggc aggtcttcgc cgttgccgcc
     1201 gaccagccca agccgcacgg ggatgggaac gggttttctt ttcgcgccct ccggcacagg
     1261 aggataggac tggctcagag tgagacgcgc ggatttcgtg ccttgcgaat aggtcaggcg
     1321 cgcgtcgaga gagggtgtgc ccgcctgatt gtaccagagc atgaactcag acagatcggt
     1381 cttgtttgca tcgccgagcg cggagacgaa atcctcgacc gtcaccgcct gcccatcgtg
     1441 gcgcttgaag tagagatcga ggcctcgccg gaaaccatgc ttgccgagca cggtttgcag
     1501 catccggcaa agttcggcgc ctttgttgta gaccgtccgg gtgtagaagt tattgatttc
     1561 aatgaaggag gaggggcgcg cggggtgagc gagcggaccg gcatcttcgg ggaactgggc
     1621 gctcttgagg aatcggactt cttggatgcg cttcacgacc gccgagcgga catccgcgct
     1681 gaactcctgg tcgcgaaaaa ccgtcaagcc ttccttgagg cacagctgga accaatcgcg
     1741 gcaggttacg cggttgcccg tccaattatg aaaatattcg tgcgcgatca cggcttcgat
     1801 gctttcgtaa tcggcatcgg tcgcggtttc gggccgggcc aggatcaggg cgtcgttgaa
     1861 gacattcagg cccttgttct ccatcgcgcc catgttgaag tccgagacgg ccacgatcat
     1921 gaacgtatcg agatcgtatt cgaggccgaa gcgctcttca tcccatttca tggagcgctt
     1981 aagcgactcc atcgcccagg cgcagcggtc atccttgccg ggttcgacat agatccgcag
     2041 atcgaccttg cggcccgaac gggtcgtaaa gctgtcctgc accatgccga gctttccggc
     2101 gaccagcgcg aagagatagg agggcttcgg aaaggggtct tcccaaaccg cgaagtggcg
     2161 gccggggccg gcctcaccgc tctcgatgag gttgccattc gaaagcagga tcggcgcgtc
     2221 tttccggtct gcttcaatgc gcaccctgaa tttcgacaag acatcaggcc tgtcgaggta
     2281 atacgtgatg cggcggaaac cttcggcctc gcactgcgtg caatagacgc cctgcgaccg
     2341 gtagaggccg ctcagggcct tgttcgcttc gggatcgcag atcgtctcga tttcgagcgt
     2401 gaacggcgcg gatggcaccg tctcgattgt cagatctttc tctgtgagaa catagcgtga
     2461 tgcgggcaag tcctggccat cgatagtcac acgcataagc tgcagcagct cgccgtcaag
     2521 cgtgagcggg tgcgtcttgc ccttaacctt cgggttcgga tgcacggaaa gtctggcctt
     2581 cacactcgtt tgcgaagggc tcagagccac atcgagctcg gtcttggaaa tcaggaaggg
     2641 cggcggcgta taatctttga gataaatggt ccgaggcagg tcagttttca tacgttcgtc
     2701 ctcaatcacg acgcttgact aaaccgcgcc gaactctgtg caaaggcctt tttccgcctt
     2761 ttgcccgtcc acacaggcgt tgcggacaga tagtgactta acggcgagtt gacaggactg
     2821 ccctgatcaa aggcgaaagc ctcaggtttc gtcccgatct ttttaaatcc cttgaccgcg
     2881 accggcgaca cagaagacct tggggccagc gaatttggaa ccggccgcct catggagaaa
     2941 aagaacaccc gcatccttgg cctcgatccc ggcctttgca atctcggctg gggcgtcgtc
     3001 gattgggatg gggcgcgcct ttctttcgtg ggttgcggca ccatcaagac ggacgcggac
     3061 gagccgatgg ggcaaaggct tgcaactctc ttcgcaggtc ttgaggaggt ggtgcagcgc
     3121 tggctgccca tggaggccgc ggtcgaggaa acctttgtga atggcaatgc gcgttcagcg
     3181 ttgaagctgg gacaggcccg cggcgtcgcg ctgcttgtgc cggcgcgcct cggtttggac
     3241 gtggcggagt acgcgccaaa caccgtcaag aagacggtca ccggctctgg ccacgccgac
     3301 aaggcgcaga ttcgggctat gatcggctat tt
//
LOCUS       DATMGX010000131         3383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_378439, whole genome shotgun sequence.
ACCESSION   DATMGX010000131 DATMGX010000000
VERSION     DATMGX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378439"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VNR65_02155"
     CDS             <1..184
                     /locus_tag="VNR65_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17507.1"
                     /translation="GQSAATMQAVRVPQSKPPLHLVLGRVAYEQVSSKLKNFSNELEA
                     WRELSLGADFPKTKSS"
     gene            complement(819..1160)
                     /locus_tag="VNR65_02160"
     CDS             complement(819..1160)
                     /locus_tag="VNR65_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWJ17508.1"
                     /translation="MASQQSIADTIVEQLAGTGVTARKMFGEFGLFHEGKMVALICDD
                     QLYVKPTGAGRAFLGDCPEGQPYPRSKPHFQIPGERWEDSEWLTKLIQLTASDLPPPK
                     LKVARSKRGTR"
     gene            complement(1253..2284)
                     /locus_tag="VNR65_02165"
     CDS             complement(1253..2284)
                     /locus_tag="VNR65_02165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ17509.1"
                     /translation="MATNLEKPDFKKQFRGMVQGAQSLTVGYIGVVNGLFADLKQLGT
                     GDVASIATAARMDAAYVRRWCDAAFAFGYLDVEGEVFRLSEMGAAMLPDAPDTLMPLA
                     MTMVSSVHMAVRASGLMRSGERPGESVMAEVETLLPWFGAMLEGTFARFFEEKICPAV
                     PVFSEINERGGLVVDLGCGNGWYLRALARRCGEVRGLGLDGFEENVAQATRLAQREGL
                     GDRLNFVQGGALKFKLDQPADLIAMNRALHHVWEGGITPFIRRLRDNLRPGGAVAIWE
                     PDWPTDRSVLRKPALRSLALHNLNEHVQGNHLLYADEIADALAAEGLAPEIHRFADGI
                     EAIIVARRI"
     gene            2453..3019
                     /locus_tag="VNR65_02170"
     CDS             2453..3019
                     /locus_tag="VNR65_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWJ17510.1"
                     /translation="MPGQAFALDVDGKTRSPVEADSSTSGAFPRTRAPEKLQIAMTVN
                     GVRTQREVAPWTTLLDFLRDHLDLTGTKKGCDHGQCGACTVLVDGRRINSCLTLAVMK
                     DGASVTTIEGLAANGSLHPLQQAFIDHDAFQCGYCTPGQICSAAGMIREGKAKSAAEI
                     RELMSGNICRCGAYPNIVAAIQQAMAQS"
     gene            3016..>3383
                     /locus_tag="VNR65_02175"
     CDS             3016..>3383
                     /locus_tag="VNR65_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HWJ17511.1"
                     /translation="MINFDYVRATGVADAVRHIAGHPGAKFIAGGTNLLDLMKEDVER
                     PQKLIDISRLALSGVEQTDSGGLRIGALTPNADLAYHPLIEQRYPILSSAILAGASAQ
                     LRNMASTGGNLLQRTRCYYF"
BASE COUNT          737 a         1028 c          967 g          651 t
ORIGIN      
        1 gggccaaagc gccgctacga tgcaggccgt tcgggttccc caatcgaagc cgccgcttca
       61 tcttgtgctc ggccgcgtcg cctacgaaca ggtctcaagc aagttgaaga acttcagcaa
      121 cgagttggag gcttggcgag aactgtcttt gggcgctgat tttcctaaaa caaaatcatc
      181 gtgagggaaa gggctgagcg caggtcgcgc tcaggctaac cccgcgacct tcgccagccc
      241 ttcgatatcg agcgactgtt cgagagccgg cgcgagttcg tccaacgccg cagcgccacc
      301 gcgggctgat taagtcattc gagatcgccc caaagttggc caggaacgac gcgggccaag
      361 aaaaccttcg tgaatagcgt gcgcccgcca gcgcaaccaa tctcaaggtg attttgccac
      421 ccccctacct tatctcacta aactatgttt ccatgcagct tctggaagtg atttcctaat
      481 agtactgcta agcacaaccc ccctagcgca cagacaaata gcactcgctg atagcgctcc
      541 ccaatcgtcg tgtacgtcat caggatggta aaaaatatta ggaccaagcc tagatataag
      601 cccacctctc gtgacgtaat tatggccggg cggagaaggc taagcgagat atctgcggat
      661 acgattgcca ggactaacat cgctattaaa tcggggatgg cctgcatcgc gctaattcct
      721 gtccgcttta taagaagtgc ttaatcataa agcgatggga gctacgccgc attacggatg
      781 agcttagatg gttaattgtg tgccgatatt gtatgtgttc aacgggtgcc gcgtttcgat
      841 ctagccacct tcagcttcgg tggaggaagg tctgatgcgg tcagctgtat cagctttgta
      901 agccactcgg aatcctccca gcgctctccc ggaatctgaa aatgcggctt ggagcgggga
      961 tagggctgcc cttcagggca atcgccaagg aaggcgcggc ccgctccggt cggcttgaca
     1021 tacagctgat cgtcgcagat gagcgccacc attttgcctt cgtggaaaag accgaattcg
     1081 ccgaacatct tgcgcgctgt cacgcctgtg cctgccaact gttcgacgat ggtatcggca
     1141 atgctttgct gcgaagccat gacgacgcga ctccggcgga acgacaactc cttatcgcca
     1201 gcccgccaac aaagatcaag atgccggggc acacggtgcg ggagttcgta ggctagatac
     1261 gccgggcaac gataatggct tcgatgccat cggcgaaccg gtgaatctcc ggggcaagcc
     1321 cttctgcggc taaggcgtcg gcgatctcat cggcgtaaag caggtgattg ccctgcacat
     1381 gctcgttgag gttatggagc gccaggctgc gcagcgcggg ctttcgcaga accgagcggt
     1441 cggtcggcca atccggctcc cagatcgcca ccgctccgcc agggcgaaga ttgtcgcgca
     1501 gccgccgtat gaacggggtg atcccgccct cccagacatg atgcaaggct cggttcatgg
     1561 caatcagatc ggcgggttga tcgagcttga actttagcgc gccgccctga acgaagttga
     1621 gccggtcgcc aagcccctcc cgttgagcaa ggcgcgtcgc ttgtgcgacg ttctcttcga
     1681 acccgtcgag accgagtccg cggacctcgc cgcagcgacg ggccagggcg cgcaggtacc
     1741 agccattgcc gcagccgaga tcgaccacaa ggccgccgcg ttcattgatc tcggaaaaga
     1801 ctggcactgc ggggcaaatc ttctcttcga aaaaacgcgc gaaagtacct tcgagcatgg
     1861 cgccgaacca cggcagaagc gtttcaacct cggccataac gctctcgccg gggcgctcgc
     1921 cgctgcgcat gaggccagag gcgcgcacgg ccatatgaac actcgaaacc atggtcatcg
     1981 ccagaggcat gagcgtgtcc ggcgcatcgg gcagcatggc cgcgcccatc tcgctcaagc
     2041 ggaagacctc gccctcgacg tcgagatagc caaaggcgaa ggctgcatcg caccagcgtc
     2101 gcacataggc ggcgtccatc cgcgcagctg tggctataga cgcgacatcg ccggtgccga
     2161 gctgcttcaa gtctgcgaac aggccattga ccactccgat atagccgacg gtcaggcttt
     2221 gcgcgccctg caccatgccg cgaaactgct tcttgaaatc cggcttctca agatttgtcg
     2281 ccatggccta tcgatccttc aagtccgatg ctccggctca gtctaagcac tagcggcctc
     2341 acgcgtatat gacagagtca cacgccatcg gaatggcgtg gctcgatgga gtaacgcgtc
     2401 atcgacattt cgctagtgca tatgcgatgc cgagcttcgg gaaggaacat cgatgcccgg
     2461 acaagcattt gctctcgatg tcgatggcaa aacgaggtca ccggtggaag ccgacagctc
     2521 aacatcaggg gcgtttccaa gaacgagagc gcccgaaaaa cttcaaatcg cgatgaccgt
     2581 caatggcgtg cggacgcaac gcgaagtcgc gccctggaca acgctcctgg acttcttgcg
     2641 cgaccatctc gacttgaccg gcacgaagaa aggctgcgat cacggtcagt gcggcgcctg
     2701 caccgtcttg gttgatggcc gccgcatcaa ttcctgcctg acgcttgccg tcatgaagga
     2761 tggcgccagc gtaaccacca tcgagggctt ggctgcgaat ggctctctgc atcccctgca
     2821 gcaggcattt atcgatcacg acgcttttca atgcgggtat tgcacgccag gacagatttg
     2881 ctccgccgca ggcatgatcc gcgaaggcaa ggcgaaatct gccgccgaga tccgtgaact
     2941 catgagcggg aacatctgcc gctgcggcgc ctaccccaac attgtcgcag ccatccaaca
     3001 agccatggcg caatcatgat caacttcgac tatgtacgcg caaccggcgt cgccgacgcg
     3061 gttcggcaca ttgcaggtca tcctggggcg aaattcatcg cgggcgggac caatctccta
     3121 gacctgatga aggaggatgt cgaacggccg cagaagctca tcgacatatc gaggctcgcg
     3181 ctcagcggcg tcgagcagac ggacagcggc ggattgcgca ttggcgctct gacgccaaac
     3241 gccgatctcg cctaccatcc gttgatcgag cagcgctatc cgattttgtc gagcgcgatc
     3301 cttgcgggcg cttcggcaca actgcgcaac atggcctcga cgggcggcaa tctccttcag
     3361 cggacgcgtt gctactactt cta
//
LOCUS       DATMGX010000132         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_366084, whole genome shotgun sequence.
ACCESSION   DATMGX010000132 DATMGX010000000
VERSION     DATMGX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366084"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..395
                     /locus_tag="VNR65_02180"
     CDS             15..395
                     /locus_tag="VNR65_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWJ17512.1"
                     /translation="MPLLIHVLVAGGVASAIVLLSYFIGQHKYNKSKMSPYECGMEPV
                     GNARQRFTVKFYLVAMLFILFDVEAVFLYPWAVVQRDLKSAGMGMFWFWEMLVYIGIV
                     LAGYWYCWKKGVLDWNKQAKSERI"
     gene            399..896
                     /locus_tag="VNR65_02185"
     CDS             399..896
                     /locus_tag="VNR65_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWJ17513.1"
                     /translation="MALEPAITDIEQLKDRPELARLLAWNAGAITGAKSDRNELTIWV
                     DRTGLREACLTLKNDPQLQYNALSDITCVDWYPKAPRFEVVYQLFSIPNKNYLRLKVK
                     ISGEDASLDSLVPIWPGADFFEREVFDLFGVRFDEHPNLKRIMMPEDWEGHPLRKDYP
                     VEGYR"
     gene            908..2104
                     /gene="nuoD"
                     /locus_tag="VNR65_02190"
     CDS             908..2104
                     /gene="nuoD"
                     /locus_tag="VNR65_02190"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HWJ17514.1"
                     /translation="MTPTPVLDPSDDKTMVLNMGPQHPSTHGVLRLLLEIDGETIVRI
                     MPDIGYLHTGIEKTCEAKFYQQVVPLTDRIDYLCPMTNNLAYCLAVEKLLGLEIPPKA
                     QWIRVMLNELTRINSHLVWLGTHAMDIGAMTVFLYCFREREDVLRLFEAVSGQRMMTS
                     YFRIGGLALEPPLGFFERVKSFNDRFPANIDEYESLLTSNPIWTGRTKGIALLSAEDA
                     IALCATGPTLRASGVDVDLRRDKPYSGYEKFQFNVPVSNDGDVFARYVVRVAELRESQ
                     KIVKQALEGVPEGPVKADAPHVVLPDREKMKTQMEALIYHFKIVTEGFTVPEGEVYMP
                     IESPRGEMGYYVVSDGTAKPYRVHMRSPSFANLQTLRKMCEGRLIADVVAAIGSIDIV
                     LGDCDR"
BASE COUNT          445 a          628 c          650 g          447 t
ORIGIN      
        1 acttcgcccg atatctgccg ttgttgatcc acgtcctggt agctggaggc gtggcctcgg
       61 cgattgtgct gctctcctat ttcatcggcc agcacaagta caacaaatcc aagatgtctc
      121 cctacgaatg cggcatggag cccgtcggca acgcccgtca gcgcttcacc gtgaagttct
      181 acctggtggc gatgctgttc atcctgttcg acgtggaagc cgtatttctc tacccttggg
      241 cggtcgtgca gcgcgatctg aaatccgcag gcatgggcat gttctggttc tgggagatgc
      301 tggtgtacat cggcatcgtg ctcgccgggt actggtattg ctggaagaag ggcgtactcg
      361 actggaacaa acaagctaag tccgagagga tttaagggat ggcactggag ccggcgatca
      421 ccgacatcga gcaactgaaa gatcgtcctg aactggcgcg cctgctggcg tggaatgcag
      481 gcgcgattac gggcgcgaag tccgaccgca acgaactcac gatctgggtc gaccgcaccg
      541 ggctgcgcga agcctgtctg accctgaaga acgatccgca gttgcagtac aacgccttgt
      601 ccgacatcac ctgcgtggac tggtatccca aggccccccg cttcgaagtc gtgtaccagc
      661 tgttctccat tccgaacaag aactacctgc gcctgaaggt gaagatctcc ggcgaggacg
      721 ccagccttga ctcgctagtt ccgatttggc cgggcgcaga tttcttcgag cgcgaagtct
      781 tcgacctgtt cggcgttcgc tttgacgaac accccaatct aaaacgcatt atgatgcccg
      841 aggattggga aggccacccg ctccgcaagg attatcccgt cgagggctac cgttaatcat
      901 ggcccacatg actccaaccc cggtgctgga cccgtccgac gacaagacga tggtcctgaa
      961 tatgggtccg cagcatccct cgacccatgg cgtgctccgg ttgctgctcg agattgacgg
     1021 cgagaccatc gttcgcatca tgcccgacat cgggtacttg cacaccggca tcgagaagac
     1081 ctgcgaagcc aagttttacc agcaggtcgt tcccttgacc gaccgcatcg attatctctg
     1141 ccccatgacc aacaacctgg cgtattgcct ggcggtggag aagttgctgg ggctggagat
     1201 tccgcccaag gcgcagtgga ttcgcgtcat gttgaacgag ctgacgcgca tcaactcgca
     1261 cctggtgtgg ctgggcacgc acgccatgga catcggcgcc atgacggtct tcttatattg
     1321 cttccgcgaa cgcgaagatg tgctgcgctt gtttgaggcg gtctccgggc agcgcatgat
     1381 gacgtcgtac ttccgcatcg gcggactggc gctggagccg ccgctcggtt ttttcgagcg
     1441 ggtcaagagt tttaacgacc gctttcccgc gaacatcgac gagtacgaga gcctgctgac
     1501 cagcaatccc atttggacgg gacgcaccaa gggcatcgcc ctcctatccg cggaagatgc
     1561 catcgcactg tgcgcaaccg gcccgacgct gcgggccagc ggtgtcgatg tcgatctgcg
     1621 ccgcgataag ccgtatagcg gctatgagaa atttcagttc aacgttccgg tatcgaacga
     1681 tggcgatgtc tttgcgcgtt acgtggtgcg ggtggcggaa cttcgggaat cgcagaagat
     1741 cgtgaagcag gcgctggagg gcgtcccgga aggcccggtg aaggctgacg ctccgcatgt
     1801 cgtgttgccc gaccgcgaga agatgaagac gcagatggaa gcgctcatct atcacttcaa
     1861 gatcgtgacc gagggattca ccgtgccgga gggcgaagtg tacatgccga tcgagtcgcc
     1921 gcgcggcgaa atgggctact acgtggtgag cgacggcacc gccaagccgt atcgcgtcca
     1981 catgcggtcg ccgtcgttcg ccaatttgca aacactgcgc aagatgtgcg aaggccgcct
     2041 gatcgccgac gtggtcgccg ccatcggcag catcgatatc gtgctggggg attgcgatcg
     2101 gtagcaatta gcagctagca attagcaact agtttaagcg attcaggcta agagctaaat
     2161 gcgaatacta
//
LOCUS       DATMGX010000133         2536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_317098, whole genome shotgun sequence.
ACCESSION   DATMGX010000133 DATMGX010000000
VERSION     DATMGX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317098"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VNR65_02195"
     CDS             <1..195
                     /locus_tag="VNR65_02195"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HWJ17515.1"
                     /translation="AFAELLGDREHVSLLEQTLQEEKQTDDKLTQLAERINSEASQAV
                     EETQNRRTSKQSKGASRRAA"
     gene            complement(1369..2085)
                     /locus_tag="VNR65_02200"
     CDS             complement(1369..2085)
                     /locus_tag="VNR65_02200"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ17516.1"
                     /translation="MKETRFADASEDIRGSHLYGLNDEKFGKIDEVIFDHSTGVIRYV
                     VVDTGGWLTTKEFLVPADGLRTSAKHTDDFEVSLTKNQIESFPPYDESVLGSDRKWAD
                     YEGRYRSKWVADPVMHRAETDRNITPTTQQTLGNSASKQAGSEKPGYGPGSEVPPSSA
                     AQRADLDAASSGTGRIVPAGTDSVVISNSAVGIGGRWDTFQSLLRERRKEAVAQCETC
                     SGESALKRGSESVDTLRKAV"
BASE COUNT          570 a          694 c          721 g          551 t
ORIGIN      
        1 gctttcgccg agttgctggg tgacagagag cacgtatccc tgctcgagca gacgctacag
       61 gaagaaaaac aaacggatga caaactgacc caattggcag agcgaatcaa ctccgaggca
      121 agccaagcgg tcgaagaaac gcagaaccgg cggacgtcta agcaaagcaa gggagcgtcc
      181 aggcgagcgg cctaatccca agggtggagg ggcgccgggc gcccttccac aacttttcct
      241 ttcggctgtg caaaatacag agttcacccg atagttcgat gcaccctcca acattatgtt
      301 gcaacttcgg taccccaact tgcgcggaag ccagcgccca caaggctggc aacgatgcgc
      361 cttatccgag gattttgatg atgagctctt gtactgacgg aaaacttttc gattcatgcg
      421 tggcgtggca tgtgctttat cacacggccc tgtttgaaac agatcgagac atgcttccac
      481 ggcgaattgc tgaggcggag agcgccatac ttgcccggat cagagaattg tttgcggtcg
      541 cgactgacca cattgaggaa gatgtcgttc tggacgacgc tctgtatgca ttgcgcgcgc
      601 tccggacatg tggtttaccg gaatcaactt ccgcctagcg agcaaatgca cttgggatat
      661 ctagagaata ttttcgccgt cactaggaaa aggcattctt gaggttgagg gcaaagctgt
      721 attgatgcgt gcgggcatcg gttttacatt gctgaaaacc gagtaagaga aagttgagtt
      781 tcccatgcct agcaaaagcg cggaacagcc tcttccacct gccaagaatt taaaacgagc
      841 tttcgagtgc gccgtccgtg acatctacca agcaggcgtc actggtcata tctcgcggta
      901 ccgtaccccg atcgtgcagc gctcggggac gagcgcttcc gggtctcatg tcgacgcaga
      961 gaaacgcgcg ctgtcggacg ctgcgaaggc cctggcgcaa ttgtggaaaa tctcaccgag
     1021 ttccagccgg tagggtcgcg ccacgatcac ggtggaggga tgcaaaggcg gaactttttc
     1081 tgggtctgtc cttttgcgcc aacccacaaa tgcggcgctc aacaagatca gtaagaagag
     1141 agcgacggca acggttgcca tcgctcgtcc cctgtctgga ttgcccacaa tcatagagga
     1201 aactgagatg ctgaactcgc accttttgtt ttccataatc ccggcccagc cgggtctttt
     1261 tgttgggaaa aaacaaaagc accctcaatc atcgagtcga ttaagggtgc cgacggcgaa
     1321 gatggcgctt tgcaacgcgt ggtccagacg tcgatttcgc ctggatcttt agactgcctt
     1381 gcgtagagta tcgacgctct ctgaaccacg cttcagggct gactcaccgc tgcaagtctc
     1441 gcactgcgcc accgcttctt tgcggcgttc gcgcaagagg ctctgaaaag tatcccagcg
     1501 cccaccaatt ccaaccgcgc tgttgctgat taccacggaa tcggttcccg ccggaacgat
     1561 tcttccggtc cctgacgagg cggcgtcaag atcggctctc tgtgcagcgc tgctcggggg
     1621 aacttcggac cccggcccgt atccgggttt ctcggacccg gcctgtttcg acgcggaatt
     1681 tccgagtgtc tgctgggtcg taggagtaat gttgcggtcg gtttccgcgc gatgcatcac
     1741 aggatcggct acccacttcg aacggtagcg cccttcatag tcggcccatt tgcggtcgga
     1801 gccgagaaca gactcgtcat aaggcggaaa gctttcgatt tggttcttgg tcaggctcac
     1861 ctcgaaatcg tcagtatgct tcgccgaggt gcgcaggccg tccgcaggaa cgaggaactc
     1921 cttggttgta agccagccac ccgtatcgac aacgacgtag cggatgactc cggtggaatg
     1981 gtcgaaaatg acttcatcga tcttgccgaa tttttcgtca ttcagaccat aaagatgaga
     2041 gccgcgaata tcctcactcg cgtctgcgaa gcgggtttct ttcaaagtgc cgtaatgtga
     2101 tatttctacc tcctatggat tggtccgtgt gattcaacaa taagtattgg ggcagagcac
     2161 ccaaggaaca attccccatt cccggtagag gacctcgatg gatgggtcaa atctgaccgg
     2221 gatcctccgg gtcaggttcg cgaacaggca tatcggggtt ctccggagga tccgggggct
     2281 cttgcacagg aaaaggctcc ggccctgccg ggatgcgcaa aatgtattcg aggaaatgat
     2341 ctgtattcgg atgcatgaca gtcagatgct acttatcttg tgtgaggctg cgacaaaaaa
     2401 gcgggcccag aggggcccga aaaactttct gctatctcct gaccgtttac ccgctcacgc
     2461 atggggcgaa gtgccgcttc cgctgggcga aacgaccggc gggcacggag tgcccggcgt
     2521 ggggctcatc cccggg
//
LOCUS       DATMGX010000134         4933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_317066, whole genome shotgun sequence.
ACCESSION   DATMGX010000134 DATMGX010000000
VERSION     DATMGX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317066"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VNR65_02205"
     CDS             <1..820
                     /locus_tag="VNR65_02205"
                     /inference="COORDINATES: protein motif:HMM:NF044715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HWJ17517.1"
                     /translation="GKGTKYLPNYHTLTHADVWTHYGLTPAQASLAGMNPQMFNSFLD
                     GTKTSIEMAAVANACGLDVPFDGLSFPPCGVDDLAHILRPKASGGLLDREGMVAVVSS
                     LERDGRPIFRDLRWGVFVLLKAPNKYAAACFSQYGLQVDSTGLYGAMYKPFHLIGLEL
                     SISVLNAVLRGEATGTPLEWRADVVAVAKRNLKVGDVLDGEGGYTVFGKLVPASRSLA
                     EDLLPIGLAHGVKVKRDMPAGSFVKTGDVLLKEDDLAVKTRREMEAMSRTAAAA"
     gene            853..1251
                     /locus_tag="VNR65_02210"
     CDS             853..1251
                     /locus_tag="VNR65_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HWJ17518.1"
                     /translation="MSGRFWIFIAAILGGLAVAAGAIGSHGLGDAVSADAKRIFSTGQ
                     QYHTLHALALLGVGIVLLQSEGSRARFSTWFLQGAALALLIGIICFSGGIYVQVARGL
                     GSSAGIVPLGGISLMVGWAAFAIGALGLRS"
     gene            complement(1327..1656)
                     /locus_tag="VNR65_02215"
     CDS             complement(1327..1656)
                     /locus_tag="VNR65_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17519.1"
                     /translation="MANLSPSTVAINTIAHCLANALDASSGLDASMSADLRALLRVAA
                     GEIDRVRKSNRKAAAAAKRAPAAPSGKKAKSKRARVEVTEVQQKPGKSKSRGRKAQAA
                     NGGIVTH"
     gene            complement(1777..2592)
                     /locus_tag="VNR65_02220"
     CDS             complement(1777..2592)
                     /locus_tag="VNR65_02220"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWJ17520.1"
                     /translation="MSQLTPEGEKIVAAIADRYAIGVEAVKMMLDAVARGGGSMAQFN
                     VPEFGGSGQWMRGGMTMVGDMFNNSLKATVDNLCNDLSQALNNQPNIFAPFMPSQTQM
                     QSQGSGGTSFWQSSQSGSWWPSELGSASSTGSQNNLRYAYFPGSNRLAIDDRGRVEVY
                     DTSGYDIGGFGQQQSGDASLTFTSQRGVVRVDSLPRVTTGTPAPQAWPSEASSSTQSP
                     VTPSRASPSTQPPASGDAGSILGLIEQLGQLKEKGLITEQEFSAKKAELLKRL"
     gene            complement(2679..3986)
                     /gene="hslU"
                     /locus_tag="VNR65_02225"
     CDS             complement(2679..3986)
                     /gene="hslU"
                     /locus_tag="VNR65_02225"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HWJ17521.1"
                     /translation="MTNFSPREIVSELDRYIVGQNDAKRAVAIALRNRWRRQQLDEDM
                     REEVLPKNILMIGPTGVGKTEISRRLARLANAPFLKVEATKFTEVGYVGRDVDQIVRD
                     LVEAAIGLVRDGKRKEVRAKAHLQAEERVLNALVGANASPATKEAFRKRLRDGELDDK
                     EIEVQVSDSGGGIPGFEIPGMPGSSIGMINLNDMLGKAFGGRSKTRRLNVRDSYELLI
                     NDESDKLLDNDQVIQEAIKSVENDGIVFLDEIDKICARSERVGADVSREGVQRDLLPL
                     IEGTTVGTKYGPVKTDHILFIASGAFHVAKPSDLLPELQGRLPIRVELQALTGDDFRR
                     ILREPQTSLIKQYIALMATEGVELEFTEDAIDAIADVAVEVNSNVENIGARRLSTVME
                     RVLDEISFEASDKARTKFTIDANYVKVHVGDLAKNADLSKFIL"
     gene            complement(4054..4626)
                     /gene="hslV"
                     /locus_tag="VNR65_02230"
     CDS             complement(4054..4626)
                     /gene="hslV"
                     /locus_tag="VNR65_02230"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HWJ17522.1"
                     /translation="MTPFAQSLQDPTGWHGTTILTVRKGGRVVVAGDGQVTLGQTVIK
                     ANAKKVRTLGKGNVISGFAGATADAFTLFERLESKLEMYPEQLVRACVDLAKDWRTDR
                     YLRRLEAMMIVADKNSTLVLTGTGDVLEPDRNVMGIGSGGNYALAAARALLETDFSAE
                     EIARKSMAIAADICVYTNDNIVVESLPSAD"
     gene            4712..>4933
                     /gene="hisB"
                     /locus_tag="VNR65_02235"
     CDS             4712..>4933
                     /gene="hisB"
                     /locus_tag="VNR65_02235"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012687.4"
                     /note="catalyzes the dehydration of
                     D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate to
                     3-(imidazol-4-yl)-2-oxopropyl phosphate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HWJ17523.1"
                     /translation="MRKARIERKTKETAIAVEVNLDGAGAFSISTGVGFFDHMLEQLS
                     RHSRIDITLQATGDLHVDQHHTVEDCGIAL"
BASE COUNT          949 a         1448 c         1458 g         1078 t
ORIGIN      
        1 tggcaagggt acgaaatatc tcccgaacta tcacacgctg acgcacgccg acgtatggac
       61 tcactacgga ttgaccccgg cgcaggcgag cctggcgggg atgaaccccc agatgttcaa
      121 ctcattcctc gacggcacga agacgtccat cgagatggca gcggtcgcga acgcgtgcgg
      181 tctcgatgtt cccttcgatg gcctcagttt ccctccctgc ggcgtggacg atctggctca
      241 catcctgcgg cccaaggcaa gcggagggct tctcgatcgc gagggcatgg tcgcggtcgt
      301 ctcatcgctg gagcgcgatg gtcggccgat ctttcgcgat cttcgctggg gcgtgttcgt
      361 gttactgaaa gcccctaaca aatatgccgc cgcctgcttc agccagtatg gcttgcaagt
      421 cgacagcaca ggcctctacg gggcgatgta caagccgttc catttgatcg gccttgagct
      481 ttcgatctcg gtgttgaacg ccgtgctgcg cggggaagct accggcactc cgttggaatg
      541 gcgcgccgat gtcgtggcgg tggcgaagcg gaatttgaag gtcggcgacg ttctcgatgg
      601 tgaaggcgga tataccgtct tcggaaaact cgtgccggcg tctcggtcgc tcgccgagga
      661 tctgttgccc atcgggctgg cgcatggcgt aaaggtcaag cgtgacatgc cggccgggtc
      721 tttcgtcaag accggcgatg ttcttctcaa agaggatgac cttgccgtaa agacccgaag
      781 agagatggag gccatgagcc gaacggctgc ggcagcgtga gcaccgccgg tttcatttgc
      841 tgaggtaagg gcatgagcgg acgattctgg attttcatcg cggccattct aggcggcttg
      901 gcggttgcgg ccggggcaat aggttcccat ggtcttggcg atgccgtaag cgccgatgcg
      961 aagcgcatct tctcaaccgg ccagcaatat cacactctcc acgcgctagc cttgctcggg
     1021 gtcgggatcg tcttgctgca atcggagggg agccgcgcac gcttctccac ctggtttctg
     1081 cagggtgccg cccttgcctt gctgattggc attatatgct tttcgggcgg catttacgtg
     1141 caggtcgcgc ggggcttagg ctccagcgca ggaatcgtgc cgcttggcgg tatttcattg
     1201 atggttggct gggcagcctt cgcgatcggc gcgctcgggt tgcgcagttg aaacaagagc
     1261 cggtgcccgg ccttgagacg cgagaacgaa gcgcctgcga ccggctttgt tcattctcat
     1321 gcataattag tgcgtaacga tgccaccatt cgctgcttga gccttgcggc cgcggctttt
     1381 ggacttgccg ggcttctgtt ggacttcggt cacttcaacg cgagcgcgct tcgactttgc
     1441 cttctttccg gatggggcgg cgggagcacg tttcgcggca gccgcggctt tgcgattgga
     1501 cttacggacg cggtcaattt cgcctgcggc cacgcgcagc aaagcgcgaa ggtctgcgga
     1561 catcgaagca tcgagcccac tcgaggcatc caacgcattt gcaaggcagt gtgcaatcgt
     1621 attgatcgca actgttgagg gagaaaggtt tgccatcttg attcgctttc catgaaatgg
     1681 ccctattggc caagttcaca gatagcaact tatagttgaa agtgatttac actaaaattt
     1741 tcaattgaag cgacctatcc cataaaagcg gcgcatttac aatcgcttaa gaagttccgc
     1801 cttcttagca gagaactcct gttcggtgat aaggcccttt tccttcaact gacccagttg
     1861 ttcgatcaag ccgaggatcg aacctgcgtc gccgctagca ggaggctgcg tggagggaga
     1921 cgcccgggac ggcgtaaccg gagactgcgt agaggatgaa gcctcggacg gccaagcctg
     1981 cggagcggga gtcccggtcg tgacgcgagg caggctgtcg acgcgaacaa caccgcgctg
     2041 gctggtgaaa gtcagcgagg catctccgct ttgttgctgg ccgaagccgc caatgtcgta
     2101 accgctggtg tcatacacct cgacgcgtcc gcgatcatca atggcgagcc gattgctgcc
     2161 gggaaaatag gcgtagcgga gattattctg cgaacccgtc gacgaagcgc tccccagctc
     2221 ggacggccac cacgatcccg actgcgacga ctgccagaaa cttgtgccgc cgcttccctg
     2281 gctttgcatc tgcgtctgag acggcatgaa cggcgcgaat atgttgggtt ggttattcaa
     2341 agcctgagag aggtcgttgc aaagattgtc gacggtcgcc ttcagcgaat tgttgaacat
     2401 gtcgccgacc atagtcatgc cgccgcgcat ccactgacca ctcccgccga actcgggcac
     2461 attgaactgc gccatgctcc cgccgcccct cgctacggcg tccagcatca ttttcacggc
     2521 ctcgacgccg atagcgtagc gatccgcgat cgctgcgacg atcttttcgc cttcgggcgt
     2581 tagctgagac atggcgaatt ctctctcctg gacggtagac tggattataa atggctgttc
     2641 tgatgagaag ccagtaaacc acgtcttccc gattcccgct acagaatgaa cttcgaaaga
     2701 tctgcgtttt tcgcgagatc gcccacgtgt actttcacgt aattcgcgtc tatggtgaat
     2761 ttcgtacgtg ccttgtccga agcctcgaag ctgatctcat ccagcacgcg ctccatcacg
     2821 gtcgaaagcc gccgcgcacc gatgttctcc acgttcgaat tcacctccac ggcgacgtcc
     2881 gcgatggcgt cgatggcatc ctccgtgaac tccaactcga cgccttcggt cgccatcagc
     2941 gcgatatact gcttgatcag actcgtctga ggctcgcgga ggattcggcg gaagtcgtcg
     3001 cccgtcagag cctgcaactc cacccggatc ggcaggcgcc cttgcaactc gggtaacaaa
     3061 tcggacggct tcgccacatg gaacgcgccc gatgcgatga agaggatatg gtcggttttc
     3121 accggcccgt atttcgtgcc gacagtggtg ccttcgatga gcggcaggag atcgcgttgc
     3181 acgccctcgc gacttacatc cgcgccgaca cgctccgagc gggcgcagat cttgtcgatc
     3241 tcgtccagaa atacgatgcc gtcgttctcg accgacttga ttgcttcctg aatgacttga
     3301 tcattgtcga gaagcttgtc ggactcgtcg ttaatgagga gctcgtagga gtcgcgcacg
     3361 tttagccgcc gtgttttgga gcgtccgccg aaagccttgc cgagcatgtc gttgagattg
     3421 atcatgccga tggagctgcc cggcattcct gggatctcga atccgggtat gccgccgcct
     3481 gaatcggaca cctggacctc gatctccttg tcgtccagtt caccgtcgcg aagacgcttg
     3541 cggaaggcct ccttcgtcgc ggggctcgca ttcgcgccga cgagagcatt caggacccgc
     3601 tcctcggctt gcagatgcgc tttcgccctg acctccttgc gcttgccgtc gcgcacaagg
     3661 ccgatggccg cctccacgag gtcgcgcaca atctgatcca cgtcgcggcc gacatagccg
     3721 acttcggtga atttcgtcgc ctcaactttg aggaatggcg cgttggcaag ccgagcaagc
     3781 ctgcgcgaaa tctctgtctt cccgacgccg gtcgggccga tcatgaggat attcttcggg
     3841 agaacttcct cccgcatatc ctcgtcgagc tgctgccggc gccaacggtt gcgcaacgca
     3901 atggcgacgg cgcgcttggc atcattctgg ccgacgatat agcggtccag ttcagatacg
     3961 atttctcgcg gggagaagtt ggtcatgtcg ttttcctgat gtcatggccg agccttggaa
     4021 cgatcggagg cagccatgaa gataaaatgt aggttaatcc gccgaaggca agctttcgac
     4081 aacgatgttg tcattcgtgt agacgcatat atcggcggcg attgccatgg acttgcgagc
     4141 gatttcttcc gccgagaagt ctgtttccag aagagcgcga gccgcggcca aggcatagtt
     4201 gccgccggag ccgatgccca tcacatttcg atccggctcc agcacatcgc cggtgcctgt
     4261 caaaacgagc gtcgagtttt tgtcggccac gatcatcatg gcttcgagcc gcctcaaata
     4321 gcggtcggtg cgccaatcct tggcgaggtc cacgcaagcc cggaccaact gttcggggta
     4381 catctccagc ttcgattcaa ggcgctcgaa cagcgtgaaa gcgtcggccg tcgcacccgc
     4441 gaagcccgag atcacattgc ctttcccgag cgtgcggacc ttctttgcgt tggcttttat
     4501 aacggtttgc ccaagtgtga cctgaccgtc gcctgccaca accacgcgtc cgcccttgcg
     4561 gacagtgagg attgtcgtgc cgtgccagcc ggttggatct tgaagtgatt gcgcgaaagg
     4621 cgtcatgtat cgtacctgct gttatggcgt gggatatgga aagcgccgcg aaaccgtcaa
     4681 gaagcggctt gcagcgagcg agggctgaac cttgagaaaa gcgagaatcg aacggaaaac
     4741 aaaagagacc gcgatcgccg tcgaggtcaa cctcgatggg gccggcgcgt tttcgatctc
     4801 caccggtgtc ggtttcttcg atcatatgct cgagcaactt tcccggcatt cccgcatcga
     4861 catcaccctt caggcgacag gcgatctgca tgtcgatcag caccacacgg tggaagactg
     4921 tggcatcgcg ctc
//
LOCUS       DATMGX010000135         2130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_397069, whole genome shotgun sequence.
ACCESSION   DATMGX010000135 DATMGX010000000
VERSION     DATMGX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397069"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..903)
                     /locus_tag="VNR65_02240"
     CDS             complement(286..903)
                     /locus_tag="VNR65_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HWJ17524.1"
                     /translation="MQSATFALLLLFALCLRAAPYQAEKPSPAPAEHTMGTRLHAKGI
                     PNFGEVTPNLYRGGRPSHEGLDALKDMGVNLVVDLRGANKEEEATAKKLGMQYVAIPF
                     HCPFPKDEPFAKLLRVIRENPAKKVFVHCRLGDDRTGMAVAAYRMAEEGWSAEEAMNE
                     MKAFGFTAVHHAICVGLADYEESFPERLKTSRAFKELRPCGAAPK"
     gene            complement(984..1781)
                     /locus_tag="VNR65_02245"
     CDS             complement(984..1781)
                     /locus_tag="VNR65_02245"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWJ17525.1"
                     /translation="MSGHEQFAEDLALYALNTLEGEDRANLEKHLATCSSCRLELEQL
                     RGDGALLALSVMGPKPPLRARQRLLDAVAKEAAQAAPLSQKTRKDGAPFARRSWWGFL
                     GWAATAAVIVFAASLWKENAALKQSLASASAQAAQSTRELDDLRRIAAPLMAPEAQRV
                     TLVAMKTPAQPQGKAFYLRNRSSLLFVANNMPSLPPQKAYELWLIPMQGGPIPAGMFK
                     PDAHGSATVVNPPLPAGMEAKAFAITVENEAGATTPTMPIVMMGTGE"
     gene            complement(1857..>2130)
                     /locus_tag="VNR65_02250"
     CDS             complement(1857..>2130)
                     /locus_tag="VNR65_02250"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWJ17526.1"
                     /translation="LRRRRPETDIEDVIVSVAPDLAGEADRSRAAEKVRGVLGSMPAP
                     QRSALEMAYFEGMSHSEIANKTGEPLGTIKTRIRSGLILLRKAFQG"
BASE COUNT          389 a          690 c          616 g          435 t
ORIGIN      
        1 aagcccgacg acattcgctt cacggattct ctgccgaaaa cgcgcagcgg caagatcatg
       61 cgccgcctgt tgcgtgaatt ggccacgaac ggcaacgtga aaggcgacac cacgacgctg
      121 gaagatttcg gtgtgatcgc caagctgcag gagcaggacg aagtctaagg ccgcttgtca
      181 tgctgagcga agtcgaagca tccctacctc caagcggcca ccctgcccgc ggggtggccc
      241 atcctaacgt cgcggttttt gcgacgttag ggtgggaaac caaatttatt tcggcgccgc
      301 cccgcacggc cgcagttcct tgaacgcgcg gctggtcttc agacgctccg ggaaactctc
      361 ttcgtagtcc gccaatccta cacagatggc gtgatggacg gcggtgaagc cgaacgcttt
      421 catctcgttc atcgcttcct ccgccgacca gccttcctcg gccatgcgat aggccgccac
      481 ggccatcccg gtccggtcgt cgccgagtcg gcaatgcacg aaaaccttct tcgccggatt
      541 ctcccggatc acccgcagaa gcttcgcaaa cggttcatct ttgggaaacg ggcagtgaaa
      601 cggaatcgca acgtactgca ttcccagctt cttcgccgtc gcttcttcct ctttgttggc
      661 tccgcgcagg tcgaccacga ggttgacacc catgtccttg agcgcgtcca gcccttcatg
      721 gctcggtcgc cccccacggt agagattggg agtgacctcg ccaaaattcg gaatcccctt
      781 ggcgtgcagc ctggttccca tcgtgtgttc cgcaggcgct ggggaaggct tttccgcctg
      841 atagggtgca gccctgagac agagcgcaaa caaaagcagc agcgcgaacg tggcggactg
      901 catgacgtgg aatctaagat tcatctggaa ggttagatgc ggtgctgcac actctcgaga
      961 tgtggcaggc cctcgtgcga caactactct cccgttccca tcattacgat cggcatggtg
     1021 ggcgtggtcg ctccggcttc gttctctacg gtgatggcga aggccttggc ttccatgccc
     1081 gcgggtaatg gaggattgac gacagtcgcg ctgccatgag cgtcaggttt aaacatgcct
     1141 gctggaatgg gcccgccctg catcggaatc agccacaact cgtacgcttt ctgcggcggc
     1201 aatgagggca tgttgttcgc cacgaagaga agactactgc gattccgcaa atagaatgcc
     1261 ttgccctgag gctgcgccgg agtcttcatt gccacaagcg tgacgcgctg cgcctccgga
     1321 gccatgaggg gcgcggcaat cctccgtaga tcgtcgagtt cgcgcgtgct ctgcgccgct
     1381 tgcgcgctcg ccgatgccag gctttgcttc aacgcggcgt tttccttcca cagcgatgcc
     1441 gcgaacacga tgacggcagc agtcgcagcc catcccagga agccccacca cgaacgccgc
     1501 gcaaagggtg ccccatcctt gcgcgttttt tgcgaaaggg gggcagcctg tgccgcttcc
     1561 ttcgccaccg catccagcag gcgctgcctc gcacgcagtg gaggcttcgg ccccatcacc
     1621 gacagcgcga gaagcgcccc gtcgccgcgc aactgctcaa gctccagccg gcaggagctg
     1681 cacgtcgcca aatgcttttc gaggttcgcg cggtcttcac cctcaagggt gttcagcgcg
     1741 tagagcgcga gatcctcggc gaattgttcg tgtccactca tctcatcgtc cggggtgccc
     1801 cgtccttgtc cctgcgtcct ttggagggac agggcgggga ttttgaccct gcacccttaa
     1861 ccttgaaacg ctttccgcaa caaaatcaag ccgctgcgga ttctggtctt gatcgtcccc
     1921 agcggctcgc ccgtcttgtt cgcgatttca gaatggctca tgccttcgaa gtatgccatc
     1981 tcaagcgcgc tgcgttgggg agccggcatc gagcccagca cgccgcgtac tttttcggca
     2041 gcccgcgacc ggtcggcttc gccggcaagg tcgggcgcca ccgatacgat gacatcttca
     2101 atatcagttt ccggccgccg ccgtcgcagc
//
LOCUS       DATMGX010000136         3397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_25062, whole genome shotgun sequence.
ACCESSION   DATMGX010000136 DATMGX010000000
VERSION     DATMGX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25062"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VNR65_02255"
     CDS             <1..282
                     /locus_tag="VNR65_02255"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HWJ17527.1"
                     /translation="DAILAGLKPKDIELWAVHPGGRAILDAVEAAFRLPLTALNASRR
                     TLRDFGNMSSATVLFVLKSLLEENRSGAHGCAMSFGPGLTAETMLFKAV"
     gene            291..1016
                     /locus_tag="VNR65_02260"
     CDS             291..1016
                     /locus_tag="VNR65_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ17528.1"
                     /translation="MPDFKKRDTTPELMDTEIVSYEELRGVLRELTQANELSLAYRPT
                     LSFFKRLAREGRLTEDRRIVVIDAASGYGDMSRKVDAWAAKNGVNLEIIGVDMNPWAA
                     RASSEATAPGRPLRWVTDNLFDFRPEGGADIIMSSLFTHHLPDPQLVRFIRWMEENAR
                     IGWFINDIERHPLPYYFLKVAFWATRRHRFMRHDGPVSIASAFKKEDWVRLLKEAGVP
                     EGAARVESWTPFRLCVSRVKETP"
     gene            1013..2131
                     /locus_tag="VNR65_02265"
     CDS             1013..2131
                     /locus_tag="VNR65_02265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ17529.1"
                     /translation="MKPYDAAVIGGGVAGPALAMELAREGRSVILFEKEKEAHDKVCG
                     EFISHEGVRYLHDLGVSLEGLGAVPIRHVRLARRAGAVTSDLPFEAQSLSRRVLDETL
                     LQLASEAGTEIRRGIRVKDMEQDGDAWAVNLEGADAVSAKQVFLATGKHDLKDWKRTR
                     GPHPECLAFKAYWRLKPEQAADLAGHVELILFPGGYAGLQEVEGGRANLCLIVRKDAY
                     TTRYGSWESLLDAIQGNCPHLKGRLDGATCLMDKPLAISGLPYGHVAWECGGVWRLGD
                     QAAAIPSFSGDGMSIALHSARRAARMYLKGESAEIYQQSLARQLSAQLHRATIISRLL
                     VSETGQSLAMTGAGIFPYVLPVSAGLTRIPSRALGERG"
     gene            2395..2970
                     /locus_tag="VNR65_02270"
     CDS             2395..2970
                     /locus_tag="VNR65_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ17530.1"
                     /translation="MTPIFETKRLALRPITLDDAAATQRLFPHWEIVRFLGHNIPWPY
                     PEDGALQFYRDVALPAMDRGEMWVWAIRLKDGPQHLIGCISLNLVRDNNRGFWLGLPW
                     QGRGIMQEACEVVTDFWFNVLNREKLRVAKDVDNIASRRISEKQGARLVAIEDRVFRL
                     GPTKAAIWEITREEWNARRRSSAEVELRMPA"
BASE COUNT          656 a         1005 c         1037 g          699 t
ORIGIN      
        1 gacgcaattc tcgcggggct gaagccgaag gatatcgagc tttgggccgt gcatccggga
       61 gggcgagcca ttctcgatgc ggtcgaggcg gcgttccggc tgcctttgac ggcgctcaac
      121 gcctcgcggc ggaccttgcg cgacttcggc aacatgtcgt cggccaccgt gcttttcgtg
      181 ctgaaatcgc ttctcgagga gaacagatcc ggggcacacg gctgcgccat gtccttcggt
      241 ccgggcctca ccgcggagac gatgctcttc aaggccgtct aaggagcgct atgcccgatt
      301 tcaagaaacg ggacacgacg ccggagctga tggataccga gattgtcagc tacgaggaac
      361 ttcgtggagt gctccgcgaa ctcacccagg cgaatgagct atccttggct tatcgcccga
      421 cgctttcctt cttcaagagg ctggctcgcg aaggccgcct caccgaagat cggcgtatcg
      481 tcgtcatcga cgcggcgagc ggctatggcg acatgtcgcg taaggtggat gcctgggcag
      541 ccaaaaacgg cgttaacctc gagatcatcg gcgtcgacat gaatccgtgg gcggcgcgtg
      601 catcgtcaga agcgacagcg cccggccgtc ccctccgatg ggtgacggac aacctcttcg
      661 acttccgccc cgagggcggc gccgacatta ttatgagctc tttgttcacg catcatctgc
      721 ccgatccgca gctcgttcgt ttcattcgct ggatggagga gaatgcccgc atcggctggt
      781 tcatcaacga tatcgaacgc caccccctgc cctactactt cctcaaagtc gcgttctggg
      841 ccacgcggcg gcatcgcttc atgcgccatg acggcccggt gtcgatcgcg agcgccttta
      901 agaaagagga ctgggttcgg ctgctgaagg aagcgggggt gccggaaggc gccgcaaggg
      961 tcgagtcatg gacgccgttt agactttgcg tctcaagggt aaaggagacc ccatgaaacc
     1021 ctatgatgcc gctgttatcg gaggaggcgt ggcagggcct gcgctggcga tggagctggc
     1081 gcgtgaaggg cgctccgtca ttcttttcga gaaagagaaa gaggcccatg acaaggtttg
     1141 cggggaattc atcagccacg agggcgtgcg ctatctccac gaccttggcg tttcgcttga
     1201 agggcttggc gccgttccca tccgacatgt gcgccttgcc aggcgcgccg gcgccgtaac
     1261 atccgacctt ccgttcgagg cccagagctt atcgcgtcgc gttctcgatg agaccctttt
     1321 gcagctcgct tccgaggccg ggaccgaaat ccggcgcggc attcgcgtca aggacatgga
     1381 gcaagacggc gatgcctggg cggtcaacct cgaaggcgcc gatgctgtta gcgcgaagca
     1441 ggttttcctc gcaaccggca agcacgactt gaaagactgg aaaaggacgc gcggccccca
     1501 tcccgagtgc ctcgccttca aggcatactg gcgtttgaag cccgagcaag cggctgatct
     1561 ggcgggccat gtcgagctga tcctgtttcc gggcggctat gccggcctcc aggaggtcga
     1621 aggcggccgc gcaaatctct gtctcatcgt ccgcaaggat gcgtacacca cccgctatgg
     1681 atcgtgggag agtctcctag atgccattca gggcaattgc ccgcatctga aaggccgcct
     1741 cgacggagcc acctgcctta tggacaagcc actcgcaatc tctggcctgc cctacgggca
     1801 cgtagcgtgg gaatgcggcg gcgtctggcg tctcggcgat caggcggcgg cgatcccatc
     1861 gttctcgggc gatggcatgt cgatcgcgct ccacagcgcc cggcgggcgg cgcgcatgta
     1921 tctcaaaggg gaaagcgcag aaatttatca gcaatccctc gcgcggcagc tttcagccca
     1981 gcttcaccga gcgaccatca tctcgcggct ccttgtcagc gaaacgggcc agagcctggc
     2041 gatgacaggc gccgggatct ttccctatgt cctcccggtc agcgcgggcc tgacgcgaat
     2101 cccgtcccgc gcgcttgggg aacgcggctg agaggcgctc gcctatcatg catgatggca
     2161 taatgcatca cggctggatg tttggagtag gctgggcaca cctccttctg gcggctgatg
     2221 gcgctcgttc tcgccggagt gatcgaatac attttttttg actgaacccc ctccccgcgc
     2281 ggaccacatc acaatttctc ttgttgaaag agtgctctgt tctttcacga gcgttagtct
     2341 tgcaagcgca caaaggctat tagcacttct ttattgcgta acctggtctt gcccatgaca
     2401 ccgattttcg aaaccaagcg gctggcgttg cgtccaatca cactggacga cgcagcagct
     2461 acccagaggc tttttccgca ttgggagatc gtccgctttc tggggcataa tatcccctgg
     2521 ccctatcctg aagacggcgc cctccaattc tatcgtgatg tggctttgcc ggccatggat
     2581 cgaggtgaaa tgtgggtctg ggcgattcgc ttgaaagatg gaccgcagca cctgatcggc
     2641 tgcatcagcc tcaacctcgt gcgcgataat aatagaggct tctggctcgg cttgccctgg
     2701 caagggaggg gcatcatgca ggaggcttgc gaggtcgtga cagacttctg gttcaacgtc
     2761 ctcaaccgcg aaaagctgag ggtggcgaag gacgtggaca atatcgcatc gcggcgtatt
     2821 tcagaaaagc aaggcgcgcg cctcgtcgcc atcgaggacc gcgtcttccg cctaggtccc
     2881 accaaggccg ccatctggga gatcacaagg gaagagtgga acgcgcggcg gcgaagttca
     2941 gccgaggtgg agcttagaat gccggcctga gcaaaagccg ccactctggc cggaggattg
     3001 tcgtctcggc ttacaggcca gctggctttt tgtatgctcg cttgcaaaag gcggcatctc
     3061 gctcgaaccc tgccaaccgc cggagcgttc gcctcaacgc gccgtgagag cttgtccagg
     3121 cgcgccatcg tgtgaggttt ataccgggtt aaaattggtt tcgttttgta aagtcactat
     3181 aggtaaatat ataactagtt ttcctgttcg ccaaggaggc cgatggccac tcgcgtcctc
     3241 atttacggcg gttcgcatga gattcgcggc ggctcgcaca agaccggctc gggcttgcgt
     3301 ttgcgtccgc agtcctgggc ggatctcccg caaaaaagac gccttgtcga gcggcttaca
     3361 cgataagtaa ctgatttgta taggaaataa cgcgaga
//
LOCUS       DATMGX010000137         2857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_148811, whole genome shotgun sequence.
ACCESSION   DATMGX010000137 DATMGX010000000
VERSION     DATMGX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148811"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VNR65_02275"
     CDS             complement(<1..478)
                     /locus_tag="VNR65_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWJ17531.1"
                     /translation="MQKEVASTYPDGRPVGFARRWLFSTNHKDIGTLYLIFALMAGIV
                     GAVLSILIRMELAYPGIQIFSSAQSYNVAVTSHGIIMIFFMVMPAMIGGFGNWLVPLM
                     IGAPDMAFPRMNNISFWLLPASFTLLVLSLFVEGAPGSNGFGGGWTMYAPLSTSGHP"
     gene            complement(524..1366)
                     /gene="coxB"
                     /locus_tag="VNR65_02280"
     CDS             complement(524..1366)
                     /gene="coxB"
                     /locus_tag="VNR65_02280"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWJ17532.1"
                     /translation="MTARVLSLVATLCLAMFSHVEDAFAAIGAPSDWQINLPDSVTPM
                     LDQIKSFNELVLIIIVAVVLFVMGLLAWVMIRYRESVHPVPSRLSHNTTLEVLWTIVP
                     VLVLAVIAIPSFRLLFHQFDFPKPDLVVKATGKQWYWSYEYPDAKVAFDSYPVDDKDL
                     KPGQIRNLSVDNDMVVPVNKVVQVLITGGDVIHQWVVPSFGTRADGIPGRINRTWFLA
                     RELGIYYGECSALCGQGHAYMPIAVRVVSDEDFSKWISEKQKSASLEKNQMAAADPAP
                     VPAR"
     gene            1613..2125
                     /locus_tag="VNR65_02285"
     CDS             1613..2125
                     /locus_tag="VNR65_02285"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HWJ17533.1"
                     /translation="MFHPAKILAACFFLALPVHQAHAQSEVGSGQVKAVYGAWKLKCA
                     QIAGAKQDKCALVQDLKLQDRPNMFLTVLFMRAFDSDKKILRIVAPLGVVLPAGLGLR
                     VDGADVGNVKFLKCWKFGCLAEVIVDDTLVTKFTSGQTASFIVFPTPESGIGFPAPLA
                     GFAEGLKGLN"
BASE COUNT          661 a          788 c          795 g          613 t
ORIGIN      
        1 cgggatggcc cgatgtgctc agcggcgcgt acatcgtcca gcccccgccg aacccgttgg
       61 agcccggcgc gccttcgacg aaaagggaga gaaccaggag cgtgaacgag gcaggcagaa
      121 gccaaaacga gatgttgttc atgcgcggga acgccatgtc cggcgcgccg atcatgagag
      181 gcacaagcca attcccgaag ccgccgatca tggccggcat caccatgaaa aagatcatga
      241 tgatcccgtg gctcgtgacc gcgacgttat aggactgggc gctcgaaaag atctggattc
      301 ccggataagc cagctccatg cggataagaa tcgacagaac cgcgccgaca atgccagcca
      361 tgagcgcgaa aatcagataa agggtgccga tatccttgtg gttcgtagaa aagagccagc
      421 gcctggcaaa gcccacgggt cggccatcgg gatatgtcga tgcgacttct ttttgcatag
      481 tatgcccctc agctcgcgcc gctgcggtcc tcaaccctgg taactaacgc gcgggaaccg
      541 gcgccgggtc cgcggccgcc atctggttct tttccaggct tgcgcttttc tgcttctcac
      601 tgatccactt gctgaaatct tcgtcgctca cgaccctcac ggcaatcggc atataggcat
      661 gcccctggcc gcaaagcgcc gagcattcgc cgtaataaat gcccaactcc ctcgcgagaa
      721 accaggtgcg gttgatacgg cccggtatgc cgtccgcgcg ggtgccgaat gagggcacga
      781 cccactggtg gataacgtct ccaccggtga tcaggacctg cacgaccttg ttcacgggca
      841 cgaccatatc attatcgacg gacaggtttc tgatctgccc gggcttcaaa tccttgtcgt
      901 cgacagggta ggaatcgaag gccaccttcg cgtccgggta ttcgtaagac caataccatt
      961 gctttccggt tgctttcaca accaagtcgg gtttcggaaa gtcgaactgg tggaagagaa
     1021 gcctgaatga agggatggca atcactgcca gaacaagcac cggcacgatg gtccaaagca
     1081 cctcaagcgt ggtgttgtgc gagagcctgg acggaaccgg atgtacgctt tctcggtagc
     1141 ggatcatcac ccacgccagc aaacccataa caaacaagac gacagcaacg atgatgatga
     1201 gcacgagctc gttgaagctc ttaatctggt ccagcatggg cgtgacagaa tcgggaagat
     1261 tgatttgcca gtcgctaggc gcaccaattg cggcaaacgc atcctcgacg tgcgagaaca
     1321 tagctaagca caaggtggct actaacgaga gaactcgtgc cgtcatatac acaccttctc
     1381 ttgctttatt ctaccaagtc agagctttga cttgaagttg caaggagccc ctatctactt
     1441 tccttgtgcg gattaacacc acagaggtgg tttcgtttca caactgcgtg tgaggcttgg
     1501 gctgcacaac gacgcggcat tgagcaggct gcagcctcag tctcaattcg gcccaattcc
     1561 ttgccgactt gcgaggtata gaacggcttg ctcctcaaca tggtgtgcaa taatgtttca
     1621 tccggccaag atcctggctg cctgcttttt tctcgctctt cccgtccatc aggctcacgc
     1681 tcagtccgag gttggctccg ggcaggtcaa ggcggtctat ggggcctgga agctgaaatg
     1741 cgctcagatc gccggagcga agcaagacaa gtgcgcgctt gtgcaggacc tcaagctcca
     1801 ggatcgtccg aacatgtttt tgacggtcct gttcatgcgg gccttcgaca gcgacaaaaa
     1861 gatcttgcgc atcgtggcgc cgttgggcgt cgtccttccc gcaggtctgg gattgagggt
     1921 ggatggcgct gatgtcggca acgtgaaatt tctgaaatgc tggaagttcg gctgcctcgc
     1981 ggaagtgatc gttgacgaca cgcttgtgac caagttcacg tcgggtcaga ccgcaagctt
     2041 catcgtgttc ccgacgccgg aatcgggcat cggcttcccg gctccgcttg ctggtttcgc
     2101 cgaagggttg aagggcttga actgatggat gcggagatag gcaagtttag ccaaggttaa
     2161 gcacacggcc cgtacttcag cgcgccaacc tcagcggcgg tgatcccggc gcgacggcaa
     2221 ctaaagtgcg tccggaaaag tgcgaagcgg ttttccgata aagacgcacg acaaagcaag
     2281 aacttagagc ccgcttttga tcccatcaaa agcgggctct aagtatgctt cgccgatccg
     2341 gccatgccat cttggacggc gctcgccgca cgcccttatg gtaaaccgca tcgccgcgcc
     2401 gatgtgttaa tccgaaggtg gttaattttc gcgcgccgga attatttctg gtccaattca
     2461 tactttatta atggttcatt tctcgaaaag cgaggagcgc cgcgcctacg actggccgta
     2521 gacaagctga tctagatcat tgataacaaa tagctttctg tcttagagct agccccatta
     2581 aatagaattt aataccggaa ttttctcatt aactggacgc gcaaatgtcc gttaaatatg
     2641 tttcgaacac gcgaattaac cacgcgcacg gccgaaacag cagcggcaca ggcggctgcg
     2701 atggcgtccc gttcggtatt ttttctcgca ttatcaattg gttgagcggg cgtgaggtct
     2761 cgttaacctg ccggcaaggc gaattcgctg atgatctttc agaacaagaa ttgcggacga
     2821 aagcgtcagg gacaagttaa aaagcagatg tcgcgta
//
LOCUS       DATMGX010000138         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_316981, whole genome shotgun sequence.
ACCESSION   DATMGX010000138 DATMGX010000000
VERSION     DATMGX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316981"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..661)
                     /locus_tag="VNR65_02290"
     CDS             complement(104..661)
                     /locus_tag="VNR65_02290"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWJ17534.1"
                     /translation="METISTHHKGKRLRLFFQDEARIGQKGRVCHIWWKRGERPPGLC
                     DRRFTFAYIFAAVEPGTDNAFALMMPYADTAAMQAFLDRFSETLAEDEHAVMVLDQAG
                     WHGSNALAVPANVTLVPLPAYSPELNPVERVWLYLKERFLSHRLLDDYDAIVDAACNA
                     WNSLLAETGRIRSLCSYPWIPAVSV"
     gene            complement(636..1160)
                     /locus_tag="VNR65_02295"
     CDS             complement(636..1160)
                     /locus_tag="VNR65_02295"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWJ17535.1"
                     /translation="MAALTIRGDRTPTVLRKLAKAEANTRVARRLLAIANALSGMSRK
                     EAAEAAGMDRQTLRDWVIRYNAYGPDGLYDCWGDGRPPRLEPDEQAELIRLVLEGPGP
                     ESGLSAFTREDLVRICEAHFGKTFHPASLGRLLKRLGFSRQKARPSHPKKDPAQAEAF
                     KRGFGAGGNHQYTS"
     gene            complement(1259..2465)
                     /locus_tag="VNR65_02300"
                     /pseudo
     CDS             complement(1259..2465)
                     /locus_tag="VNR65_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239636.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          530 a          860 c          795 g          540 t
ORIGIN      
        1 agtgaccgcc cccggcagca aggctgcaaa cccggccttc gacgtgacgc cggcgcgcct
       61 gatcaccggg ctcatgacgg aacggggtgt tataccaccg agcttaaaca ctgaccgctg
      121 gaatccaagg ataggagcat agcgacctta ttcgcccggt ctccgccagg agactgttcc
      181 aggcgttgca ggcggcgtca acgatggcgt cgtagtcgtc gagcagccga tgcgagagga
      241 agcgctcctt gagataaagc cagacgcgct cgaccgggtt cagttcgggc gagtaggcgg
      301 gcagcggaac gagcgtgacg ttggctggaa ccgcaagcgc attcgagcca tgccagcccg
      361 cctgatcaag caccatcacg gcatgctcat cctcagcaag cgtctcggaa aagcggtcga
      421 ggaaagcttg catcgccgcc gtatcggcat agggcatcat cagcgcgaaa gcattgtccg
      481 tgcctggctc cacggctgcg aagatgtagg cgaaggtgaa gcgcctatcg cagagccccg
      541 gcggacgctc cccgcgcttc caccagatat gacaaacgcg acccttctga ccgatgcgcg
      601 cctcgtcctg gaagaacagg cgaaggcgtt tgcctttatg atgtgtactg atggtttcca
      661 ccagcgccga agcccctttt aaaggcctct gcctgggccg ggtctttttt agggtggctg
      721 ggccttgcct tctgccgcga gaagccgagg cgctttaaca gccgccccag cgaggctgga
      781 tggaaggtct tgccgaagtg ggcttcgcag atgcggacaa ggtcctcgcg cgtaaacgcc
      841 gaaagcccgc tttctggacc gggaccttca agcacgaggc ggatcagctc tgcctgctcg
      901 tcaggttcaa gccgcggcgg gcgcccatcg ccccagcaat cgtaaaggcc atctgggccg
      961 tacgcgttgt agcggatcac ccagtccctc aaggtctggc gatccatgcc cgccgcctcc
     1021 gcggcttcct tccggctcat gccagaaagc gcattggcga tcgcaagcag ccgccgcgcc
     1081 acgcgtgtgt tcgcttcggc ctttgctagc ttgcgcagca cagttggtgt ccgatcccct
     1141 cttatggtca gcgccgccat aatgcctttc cctccgaatc acggcgagag gggatcacga
     1201 tctgccgcgc ctgggaatcg cagcagcgca acaagagtca gtctttcggt acgccggtat
     1261 gatagatcgc cgtgagcagg gaagcggcca ctgcgcatgc agctttctta gggccgcgtc
     1321 gggttttgag ccgattgaac tgcgccttgt agtagctgtc tttcttgcgg ctggccgccc
     1381 aagcgcattg cacaagcatc gttttcagcc agggtgcgcc cttgcgcagc cgtgaggatt
     1441 tgcgtttgcc cgcgctttcg ttttggccgg gacacaagcc agcccaggca acgagatgtc
     1501 cagccgttgg aaagcggctc atgtcgcggc cgatttcggc gaggatcgtg gtcgccccca
     1561 aagtgctgac gccagggatc gtgctcagaa gctcaatcaa agaccggaaa gggctttggc
     1621 ctgagccggc agcctcctta tccattcggg cgatagcctc gtccacttgc tggtcaacgt
     1681 cccggatgga gcatgccaag gcatcgtatt gtcccagatg aagccgcagc ataaaccggt
     1741 gatgatcggt caggcgtccg tgtagagcgt cgtacagcgc ctttggctta gccttgatcc
     1801 gcccgctcgc caaggccgcc agcttgcgcg gatcgcgcac gcccgcaatc atttgcgctc
     1861 aagtagcgcg ccgtgcggta gcgcgccgct taaatgcctc gatcatccgg cggccagacg
     1921 cgcccatgac atcgctgatc accgagtcga gcttgatgtt ggcttcttca agggtctttt
     1981 gaatccgttg cacgtggctg acctgctcgc gaaccagctg cttgcgcgcc cgcagcaggg
     2041 agcgcaactc ctggatcgct tcttccggaa caaagctcgc cttgatcagg ccgcaggccg
     2101 ccaaatcggc gatccacatg gcgtcgttta tgtccgtctt ccgacataag tgagcgcttg
     2161 ccgctcacga taaacgttcg ctcttgaaca ttcttgatgt gcgctgcatt ggcgaggacc
     2221 aactcgaact tgccatcgct caggatgttc cacaccggct tccaataaac tccggtggct
     2281 tccatcgcaa catgcgtaca tccgttctct gtcagccagg ccaacagaga tttgaggcca
     2341 ctcgttgtcg tttcgaacgt ccggcattct cgcgatacct tgccgcccgc cactatccgc
     2401 acacaggcga cagccgtgtc cttgtgaaca tcaaggccgg ccacccgctc atacatgact
     2461 tccatcggtt ttccttccac accgccgcca gcgtggggac ctcgttgaaa aagaagtcta
     2521 acaaacgtgc tccggggcat tgctgccctt tggctccatt aggggtgatc gaggcgcccc
     2581 gggtccaact aagcaacggg ctcgtccgca ccatagacaa accgacctcg ttgccagcga
     2641 cggcgccaat tatagaatag acagaaggcc caatgccaac acgtttcatc catcggggtc
     2701 ggccgtaagg ccggtggaga actaa
//
LOCUS       DATMGX010000139         2309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_442672, whole genome shotgun sequence.
ACCESSION   DATMGX010000139 DATMGX010000000
VERSION     DATMGX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442672"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VNR65_02305"
     CDS             complement(<1..236)
                     /locus_tag="VNR65_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17536.1"
                     /translation="MTDPAVAPETPDVAETVSFLRRFADMMANGHNGSYLRRAADLLE
                     SLTARAVSASDEEGVWRDKYEALTQHADALETEC"
     gene            complement(423..980)
                     /locus_tag="VNR65_02310"
     CDS             complement(423..980)
                     /locus_tag="VNR65_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17537.1"
                     /translation="MATAKQIAANRANAARSTGPRTPTGRAKSSRNAFRHGLSLPRDL
                     TTATEIDRLAQAITGENPSVQQLMAANELATAQYELMRIRALRFAATPALSATLDPRR
                     LRWILGFDRYEQAALSRRKRAAKTLDRLDLPVGLPRATGLLQQGLQNEANPAGNRSAL
                     TKRTQFVGNVVGNFTARCRPPRIPR"
     gene            complement(999..1661)
                     /locus_tag="VNR65_02315"
     CDS             complement(999..1661)
                     /locus_tag="VNR65_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17538.1"
                     /translation="MARDGTTHVTGTASDAPQPSASAHSDKMPETGDLSPKATADGPS
                     IADRFWQAPVLAAGEKIEDYRALEAEISAAMQPQTFFERLHASDACHALWEEQRFRRQ
                     QAALPGASRMKALVCLLASIGFEKDALDIATSYFGEDDDERNKATALIRRFGITDDAI
                     NAQASQQNLPILSVLEHLMANRQSRRDVVVSEYHRRKRKADKSRKYVAEPQSDRPATA
                     RH"
     gene            1881..>2309
                     /locus_tag="VNR65_02320"
     CDS             1881..>2309
                     /locus_tag="VNR65_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17539.1"
                     /translation="MPARYVDQVQKLDIRSMMAAALKAGPSDHYNVYQDGSGWHAATN
                     LQVVSRARDLVAYIKLDRSAAKGAARGQILLTVVQRDGRSCEQALWLRAEATQRGRTR
                     WIALCPCSHQPAQTLYFDMGVQQFVSRQVAGLKYRRAVRKV"
BASE COUNT          434 a          686 c          697 g          492 t
ORIGIN      
        1 tcgcattcgg tttccagcgc atcggcgtgc tgggtcaagg cttcatactt gtctcgccag
       61 acaccttcct catccgaggc tgacacggcg cgcgccgtca ggctttccag aagatcggca
      121 gcccggcgca aataggaccc gttgtgcccg ttggccatca tgtcggcgaa ccgacgcaag
      181 aagctgacgg tttccgcgac atcgggggtt tcgggcgcca cagcagggtc ggtcatgaag
      241 ctcgctttcc tggacgccat cccatacgcg tggtgatcaa cgcgttacag ccccactcgg
      301 cgcggtccaa ccaaaataaa ccggttccag accgcaaagc tcggcgatcg gatttggctt
      361 gcatgcggtt ctccagattg gcggcggcga aaatagggcg tcggcgccaa ataacgtgac
      421 ggtcatcggg gaattcgagg cgggcgacat cgcgccgtga agttgcccac gacatttccg
      481 acaaattggg ttcgttttgt aagtgccgat cgattgccag caggattggc ttcgttttgt
      541 aaaccctgct ggagtagccc ggtcgctcgc ggcagcccca ccgggaggtc gagccggtca
      601 agcgttttcg cggcacgctt tcgcctggac aaggccgcct gctcgtagcg gtcgaatccc
      661 aaaatccagc gcaagcgcct tgggtccagc gtagccgaaa gcgccggcgt cgctgcaaag
      721 cggagcgctc gaattcgcat cagctcatac tgcgccgtcg caagctcgtt cgccgccatg
      781 agctgttgca cggacggatt ctcgcccgta atggcctgcg ccagccgatc gatctcggta
      841 gccgtggtga ggtcccgcgg cagcgacagg ccgtgacgga atgcgttccg gcttgatttt
      901 gcccggcctg tgggggttcg gggaccggtg ctgcgcgcgg cgttcgcccg gttggctgca
      961 atttgcttgg cggttgccat cgctggtttc tccctctgtt agtgcctcgc cgtcgcaggt
     1021 cgatcgcttt gcggctcggc gacatatttg cgcgatttgt cggccttgcg cttgcggcgg
     1081 tggtattcgc tgacaacgac atcgcgccgc gattgccggt tggccatcaa gtgttccaac
     1141 actgagagaa ttggcagatt ttgctggctt gcttgggcgt taatggcgtc gtcggtgata
     1201 ccaaagcggc ggattagcgc ggtcgccttg ttgcgctcgt cgtcatcctc gccaaaatag
     1261 ctcgtggcaa tgtcgagcgc gtccttttcg aaaccgatcg acgccaaaag gcacaccaag
     1321 gctttcatgc ggctcgcacc cggcaaagcc gcctgttgac ggcgaaaacg ctgctcctcc
     1381 cacagggcat ggcaagcgtc gctggcgtgc agtcgctcaa aaaaggtttg cggctgcatc
     1441 gcggccgaaa tctccgcctc cagcgcgcga tagtcctcta tcttttcgcc cgcggcgagg
     1501 acaggggcct gccagaacct gtcggcaatg gacggtccat cggctgtggc cttggggcta
     1561 aggtcaccag tttccggcat cttgtcggaa tgggccgaag cagagggctg aggcgcgtcg
     1621 gaagccgtgc ctgttacgtg agttgtgcca tcccgagcca tatcaaaatc tcctctttta
     1681 ggggtttaga acagcttagg agttatcgct gctaacccca aattacaacc ccagcgagtg
     1741 caatttattt ttccattatc tcactcattt cactcgactt atctctgcat cggagtgaca
     1801 atttgcgata aggatttttc gttcaataca acttcggagt gaatagctcc aggcatcaag
     1861 gaaagcagtg aggtctcgca atgcccgccc gctacgttga ccaggttcag aagctcgata
     1921 tccgctcgat gatggccgcg gcgctcaaag ccggcccaag cgatcattat aacgtttacc
     1981 aggatggctc aggctggcac gccgctacca acctccaagt cgtgtcgcga gcacgggact
     2041 tggtcgccta catcaaactc gacaggtcgg cagctaaggg cgccgcgcga ggtcaaatat
     2101 tgctaacggt ggttcagcgc gatggcagga gctgcgaaca ggcgctttgg ctgagggccg
     2161 aggcaactca aaggggtcgc acgcggtgga tcgcgctctg cccctgcagc caccagcccg
     2221 cgcaaaccct gtatttcgac atgggcgtgc agcaatttgt atcgcgccag gttgccgggc
     2281 tgaaatatcg ccgcgccgtg cgcaaggtc
//
LOCUS       DATMGX010000140         4663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_273675, whole genome shotgun sequence.
ACCESSION   DATMGX010000140 DATMGX010000000
VERSION     DATMGX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273675"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VNR65_02325"
     CDS             <1..778
                     /locus_tag="VNR65_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17540.1"
                     /translation="VYVVDPILEGYCWSVGVLWFLTALCFVQFLAYILLQRIPAIAVV
                     LGAMAATAVVVYFDAPNYFLFRTWMPGLSFFALGYLFSQWKVPWPVWLCIPLFAAVIV
                     LAPLNSGCSFSFSGPCQLYGTAPFGIRMFGGSYGFLPLFFLTALLGSIAVVSLSSGLA
                     RFRASELFAYTGRNSLNLFIINGFVATFLPPYIQQIRWPDFTPLVSISLAIAVIAAHL
                     VALQILNPVLSWIDRAAVSIADALSRLVARGTQAAAPNAA"
     gene            complement(990..3770)
                     /locus_tag="VNR65_02330"
     CDS             complement(990..3770)
                     /locus_tag="VNR65_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17541.1"
                     /translation="MSEIPAPISDTRTAYSPAAHNAARVSRVRLFIRALGVSFFSVLS
                     VLIAFYAFYELPQVQDLLFDARPYWIQEAIYWAGFYAIGIFIWALPLVFSARLLLLQN
                     FDVIGIDTEERFKFYIFRFPSFFVVLTFVAVLVGVIAAADNLPVPLLNGGNKYEAPIR
                     SLVETHLLTLLIATAGVLVLVVFRGMFLRGYGRSMKRLEDTDPKGFKKSLIQIEELSR
                     KSDRNLEECDLHLTALKPEFLSTETWLAAQRVKGFMWRYMVRLTWFLLLLVAIHFLSY
                     SEMVQRLISLPDAYFNSQVLDLLSDTFSMKRASFLFIVFGAWIPFVTILSLLSNRYQF
                     PFISGLAAVVVGLTLFVGDGHDIRVVTISNDQQPSLRPIGFSHAVKDWKTSSGWDAKG
                     CEWLAPDANALANCPRPIIVAGEGGGSRAAFLLASVLGSLEDDSLDKSKHPSARPFHE
                     QLFAISSVSGSSVGATFFVGALKARPNTTTEDLKNALFRQRLWFPNVASANPEKANEN
                     VPTGQTITREFLTDYVSHKDALQAALSNDFISPVAISYLARDVLMLSRFPFVEDRAGI
                     LEISWEDAFNGIYGTTRETSPLSAPLQTIAPAPGGWTPLLFLNATSNETGRRIIVTPV
                     KMTEPTSTGNALFVDAYDLHELLCSPYRDPKTNAVPELSTWDRIARILPYRFSPVAHA
                     KCDNKKPTSIDIRFSTAAGASSRSPFVSPHGNVRDRRAQIADSVVDGGYFDNSGIVTA
                     LEIAQGLKSVDARLRPFILQVSSEPDWFKDNKNCGIDGSYGDRPKIPDQADFRPLGTL
                     ADVLTVNSTRVARGYETILELPDRAKQLNGGLRSVAQINVCPQPKESFFLKAIQPYTG
                     TDNAAKEQTRALHIRRKAQEEVQYKSVSLSWWLSPPLQAFLDAQIYSEHNQTERNCVI
                     SLLEDKPNQPCR"
     gene            complement(3908..>4663)
                     /locus_tag="VNR65_02335"
     CDS             complement(3908..>4663)
                     /locus_tag="VNR65_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWJ17542.1"
                     /translation="SGAAIGAAGPVTQGGAHLTNISWSIHEAEVANEIGAPCTLVNDV
                     EAAAYCLPGLASGESLVIGDVAPKLETAHRLLAANIGTGFGAAILVRTPGGWFSYPSE
                     AGHMSLPLPEDHEASLRPRFKSVEDVLSGRGLVNLHRALGNDEAALDAAEICRRAPFD
                     PKCAATISSFTQIAGDVLGNLALAFASWDGVFLFGSVAKGFAHVADHARFREAFEAKG
                     VMVEWMKRVPVALVEKQDAALFGLAALPMPPQR"
BASE COUNT         1033 a         1292 c         1336 g         1002 t
ORIGIN      
        1 ggtctatgtt gttgacccta tccttgaagg ttattgctgg tccgtcggcg tgctgtggtt
       61 tctcacagcg ctctgtttcg tgcagtttct tgcctacatc ctgctccaac gcatccccgc
      121 gatcgccgtt gttctcggcg cgatggcggc aaccgcggtg gtggtctatt tcgatgctcc
      181 gaactacttc ctgttcagga cttggatgcc ggggctttca tttttcgcgc tcggctatct
      241 attctcgcag tggaaggtgc cgtggcccgt ctggctttgc attccgcttt ttgccgccgt
      301 gatcgtgctg gcgccgttga acagcggctg ttcgttctct ttcagcgggc cctgccagct
      361 ttacggcacc gctccatttg gcattaggat gttcggggga agctacggct ttctgccact
      421 ctttttcctc accgcgctgc ttggctcgat cgccgtcgta agccttagca gcggactggc
      481 ccgcttccgg gcctcggagc tcttcgctta cactggaaga aacagcctga accttttcat
      541 catcaacggt ttcgtggcga ccttcctgcc gccctacatc cagcagatac gctggcccga
      601 ttttacgcct ttggtgtcta tctcgctggc gatagcggtg attgcggcgc atctcgtggc
      661 gctgcaaatc ctaaacccgg tgctctcttg gatcgatagg gccgctgttt caattgccga
      721 tgcgctttca cggcttgttg ccagaggaac gcaagcggca gctccgaacg ctgcttgaat
      781 tgagccgtat cggccctgct aagcaaactt cgctaatccg ggcgacggac tatcccgaac
      841 gctcgcttgg ctacgtcatc ccggccaagc gaagcgcgag ccgggatcgt aaaaacatag
      901 gcgcttcaat gtgatacgat cccggataag gcttcgcctt tccgggatga cagggcggcg
      961 atccgttggc gcgtccggca gattctgcat tagcggcagg gctggttggg cttgtcctcc
     1021 agcaacgaaa tcacgcaatt gcgttccgtc tgattatgct ccgaatagat ttgcgcatcg
     1081 agaaacgcct gaagcggtgg agacagccac cagctcaatg aaacgctctt gtactgcacc
     1141 tcctcctgcg ctttccgccg gatatgcaaa gcgcgagttt gttcctttgc ggcattatcg
     1201 gtaccggtgt atggctggat ggctttcaag aagaagcttt ctttcggctg cgggcagaca
     1261 ttgatttgag ccacggagcg aaggccgccg ttgagttgct tggctcggtc tggaagttcg
     1321 agaattgttt cgtagccgcg agcgacacgg gtggaattga ctgtcagaac atcggcgagt
     1381 gttccgagag gacggaaatc ggcttgatcc gggatcttcg gtctgtctcc ataactgccg
     1441 tcaataccgc aatttttatt gtccttgaac cagtccggct cgcttgaaac ctgaaggatg
     1501 aatggcctca atcgggcatc tacggacttg aggccctgtg caatttccag cgccgtcacg
     1561 atgccggagt tgtcgaagta accaccatcg accacgctgt cggcaatttg tgcccgccta
     1621 tcccgcacat ttccatgcgg cgacacgaag ggcgatcttg aactcgctcc cgcagccgtg
     1681 ctgaaacgaa tgtcaatgga tgtcggtttc ttgttgtcgc acttagcgtg cgcgactgga
     1741 ctgaagcgat agggaaggat ccgtgctatc cggtcccagg tgctgagttc gggaacggcg
     1801 tttgtcttgg gatcccgata gggcgagcac agaagctcgt gaagatcgta ggcgtcaacg
     1861 aacaaagcgt tccctgtgct ggttggctct gtcattttga caggagtcac gattattcgg
     1921 cggccagtct cattcgaagt ggcattcaga aacagaagtg gtgtccaccc tcccggtgcg
     1981 ggcgcgatcg tctgcaacgg cgcggacaac ggggaagtct cccttgttgt tccgtagatt
     2041 ccattgaatg catcttccca tgagatttcc aagataccgg cgcgatcctc cacaaaagga
     2101 aagcgcgata gcatcaacac gtcccgagcg aggtaggaga tcgccacggg cgatatgaaa
     2161 tcgttcgaga gcgccgcttg tagcgcgtcc ttgtggctga cataatcggt caggaactct
     2221 cttgtgatcg tctggccggt cggtacattc tcgtttgcct tctcgggatt ggcactcgcc
     2281 acgttcggaa accaaagccg ctgcctgaat aaggcattct tcaaatcttc ggtggttgta
     2341 ttgggcctgg ccttcaaagc gccaacgaaa aacgttgcgc caaccgagct gccggaaacg
     2401 cttgaaatag caaaaagctg ttcgtggaat ggtcgcgcgg aagggtgctt tgatttgtcg
     2461 aggctgtcgt cctcaagaga accgagcaca ctcgccaaaa ggaaagcggc gcggctcccg
     2521 ccgccctcac ctgccacaat gatcgggcgc gggcaatttg cgagagcatt ggcgtccggc
     2581 gcgagccatt cgcaaccctt cgcgtcccag ccgctggagg ttttccagtc ctttacggca
     2641 tgagaaaaac caatgggtcg caacgatggc tgctgatcgt tggagatcgt aaccacgcga
     2701 atgtcgtggc catcgccgac aaataacgtc aggccaacca caaccgcggc gagccccgaa
     2761 ataaatggaa actggtagcg atttgaaagc agggagagaa tcgtgacgaa gggtatccag
     2821 gcgccgaaaa cgataaaaag aaacgaggcc cgcttcatcg aaaatgtatc cgatagaaga
     2881 tcgagtactt gggaattgaa gtaagcgtcc ggcagggaaa tcaatcgctg gaccatctcg
     2941 gaataggaca ggaagtggat cgccacgagc aggagaagaa accaagtgag gcgaaccata
     3001 tagcgccaca tgaagccctt gacgcgctga gcggcgagcc aggtttccgt gctcagaaac
     3061 tcgggcttca gagccgttag atgaagatcg cactcttcga ggtttcggtc tgatttcctc
     3121 gacagctcct cgatctggat cagggatttc ttgaagcctt tgggatcggt atcctcaaga
     3181 cgcttcatcg aacggccata gcctcgaagg aacatgcccc ggaagacaac gagcacaaga
     3241 acacccgctg tcgctatgag cagcgtaagc agatgtgttt cgaccagact cctgatggga
     3301 gcttcatact tgtttccgcc attcagtaga ggcaccggga ggttgtccgc ggcggcgata
     3361 acgccgacaa gaacagctac gaaggtgagg acaacaaaga aactcggaaa ccggaaaata
     3421 taaaatttga accgctcttc cgtgtctata ccgatcacgt cgaaattctg aagcaagagc
     3481 aaacgcgccg agaagacaag cggcaacgcc caaatgaaaa tgccgatggc gtagaagccg
     3541 gcccaataga tcgcctcctg aatccaataa gggcgggcat cgaacagcag atcctgcacc
     3601 tgcggaagct cgtagaaagc gtaaaacgca atcaaaacgc tgagaacgct gaaaaaggag
     3661 acaccgagcg cccggatgaa gagccggact cgcgaaaccc gcgccgcatt gtgggctgca
     3721 ggactatacg ctgtccgggt gtctgaaatg ggcgccggaa tttctgacat tgaggccgca
     3781 ctcccttaat tggctgagaa atgtgggagg cttgggccaa ggtcgtgtcc ccatcaaggc
     3841 aacaggggac tttttgcagc atatttggcc gagccagccc atcaatcgcc gcagcgaaga
     3901 ggcgcgttca acgttgcggt ggcatcggaa gtgcggcgag cccgaacagc gcggcgtcct
     3961 gcttttcgac gagggcaacg ggaacgcgct tcatccattc gaccataacg cccttggctt
     4021 cgaaggcctc gcggaatcgg gcgtggtcag caacgtgagc aaagcccttc gcgacactac
     4081 cgaaaagaaa cacgccatcc caggaagcaa acgccaaggc gagattgccg aggacatcgc
     4141 ctgcaatttg ggtaaaagag ctgatcgtag ccgcgcattt cgggtcgaat ggtgcgcgtc
     4201 tgcaaatttc tgccgcgtcg agtgcggcct cgtcgtttcc aagggctcta tggagattta
     4261 caagcccgcg cccagacagg acatcctcga ccgatttgaa acggggccgt aagcttgctt
     4321 cgtgatcttc gggcagaggc agcgacatat gccccgcttc gcttggatag gagaaccaac
     4381 cgccaggcgt cctgacaagg atcgcggccc caaatcctgt cccgatgttc gcggccagaa
     4441 gcctatgcgc ggtttcaagc ttaggggcaa cgtcaccaat cacgagggac tcaccactcg
     4501 ccagccctgg aaggcaatag gcagcagcct caacatcgtt cacaagcgtg caaggggcgc
     4561 cgatctcgtt ggcgacttcc gcttcgtgaa tggaccagga aatgtttgta agatgagcgc
     4621 ccccttgggt gaccggcccg gcagctccaa ttgcggcgcc tga
//
LOCUS       DATMGX010000141         2026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_148650, whole genome shotgun sequence.
ACCESSION   DATMGX010000141 DATMGX010000000
VERSION     DATMGX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148650"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..507
                     /gene="fdxA"
                     /locus_tag="VNR65_02340"
     CDS             169..507
                     /gene="fdxA"
                     /locus_tag="VNR65_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HWJ17543.1"
                     /translation="MTYVVTEACIKCKYTDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPADAIKPDTEPGLEKWLEVNTEYAKSWPNITQKKESPPDAKDFEAVDGKFEK
                     YFSPKPGTGD"
     gene            1051..1674
                     /locus_tag="VNR65_02345"
     CDS             1051..1674
                     /locus_tag="VNR65_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWJ17544.1"
                     /translation="MEEPKKVLTQRQGFKTNEFVVYPAHGVGQILAIEEQEIAGAKLE
                     LFVINFMKDKMTLRVPTAKVANVGMRKLSEPVLVKKALETLKGRARVKRTMWSRRAQE
                     YEAKINSGDIVAIAEVVRDLYRSDSQPEQSYSERQLYEAALDRLSREIAVVQHVTETE
                     AVKEIEGQLAKSPRRGAKAEAEVEADGEADTEADADGDDAAVADEAA"
     gene            complement(1806..>2026)
                     /locus_tag="VNR65_02350"
     CDS             complement(1806..>2026)
                     /locus_tag="VNR65_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17545.1"
                     /translation="TVNSFRRLTLEEIQAARPLRIKLITVQPGDTVESLSHRMAGVDR
                     PAERFRVLNGLDSHAQVKVRDLVKIVVD"
BASE COUNT          471 a          600 c          615 g          340 t
ORIGIN      
        1 gcgtgttgta cgaggatttg caggaccgcc agcagtaact atctgcagaa gagcgattcg
       61 gccggcgcgg cgggtggcga aaacgccgcc cggagctgcg cttcacttgc ggcaacggtg
      121 ttcctgcgct aggcaaccca ctcaaaattg accgtttcgg agccctggat gacttacgtc
      181 gtcactgaag cctgcatcaa gtgcaaatat accgactgcg ttgaggtatg ccccgtggac
      241 tgcttctacg agggcgagaa catgctggtc atccatccgg acgaatgcat cgattgcggc
      301 gtgtgcgagc ctgaatgccc tgccgacgcc atcaagccgg acaccgaacc gggcttggag
      361 aagtggcttg aagttaatac cgaatacgcc aagagctggc ctaacatcac ccagaagaag
      421 gaatccccgc ccgacgcgaa ggatttcgag gctgtggatg gcaagttcga gaaatatttt
      481 tctccgaagc cgggaacggg agactgacgg gagaccggtt ttcacttaac cctaatcccg
      541 gtctggtcgc caaaaatgcc ccccaaacgc ccatacaggg cataaatcat tgatttttgc
      601 ggaaaatgtg ctatattggg cacattcaag ccggacccgt cagccgcgta tatggcccct
      661 atgggttccc gtgaaaaaca tcgaacaggg gcgtggcagt tccgcgcgca ggctgtgtca
      721 cagaaaacgc gtaaaaagag tgcttccaag ttaaccaaga aagccgctgc ggccagccgt
      781 agtgcaacca aaagccgtgc ccgggcttct cttaaggata cccggaccgc caccaaggcc
      841 tcggctgcca agtcctcaaa gaacaaaaga agcgcaatgc caaacaagac tgcaaaaact
      901 gccgcgaaag cgacgggttc gaaggctgct gcctcgaagg tcgcccccaa gactgctgct
      961 ccgaaaaccg ctgccgccaa gcccccgatc agcaagccgg ttgcgcacaa gcccgtcgtc
     1021 gccgccaagc cggcggccgc agcgccgcgc gtggaggaac cgaagaaggt cctgacccag
     1081 cgtcagggct tcaagaccaa cgagttcgtg gtgtatcccg cgcacggcgt tggccagatc
     1141 ctggccatcg aagagcagga gatcgccggc gccaagcttg agctgttcgt gatcaatttc
     1201 atgaaggaca agatgacgct gcgggtgccg accgccaagg tcgccaatgt cggcatgcgc
     1261 aagctttcgg agccggtgct ggtcaagaaa gcgctggaaa ccctgaaggg ccgcgcgcgc
     1321 gtcaagcgca cgatgtggtc acgccgggcc caggaatacg aagccaagat caattccggg
     1381 gacatcgtgg cgatcgccga agtggtgcgt gatctctatc gctcggattc gcaacccgag
     1441 cagtcctaca gcgaacgtca gctctatgaa gccgcgctcg accgcctgtc gcgtgaaatc
     1501 gccgtcgtgc agcatgtgac ggaaaccgaa gcggtgaagg aaatcgaagg ccaactcgcc
     1561 aagagcccgc gccgcggcgc caaggcggaa gccgaagtcg aggccgacgg tgaagccgat
     1621 accgaagcgg atgccgacgg cgacgatgcc gcagtggccg acgaagcggc ataagccact
     1681 tctccatccg cctatcaatc aagagcccgg tcgagagacc gggctttttg tttgttgtga
     1741 atctgcaact ccatcgtatc catcgtctct gcgaaagcat gcgaaagcag ggacgacgcg
     1801 agagtttagt ccaccacgat cttcacgaga tcgcgcacct tcacctgggc gtggctgtcc
     1861 agcccgttca ggacgcgaaa acgctcggcg gggcggtcga cgccggccat gcggtgcgac
     1921 agcgattcga cggtgtcgcc gggctgcacg gtgatgagct tgatgcgcag cggacgcgcg
     1981 gcctggattt cctcgagcgt caggcgccgg aaggaattga cggtct
//
LOCUS       DATMGX010000142         2373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_412860, whole genome shotgun sequence.
ACCESSION   DATMGX010000142 DATMGX010000000
VERSION     DATMGX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412860"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..2236
                     /gene="nuoG"
                     /locus_tag="VNR65_02355"
     CDS             134..2236
                     /gene="nuoG"
                     /locus_tag="VNR65_02355"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HWJ17546.1"
                     /translation="MPKLNINGTVIEVEDGITLLQACEQAGAEIPRFCYHERLSIAGN
                     CRMCLVEIGGMPKPMASCAMSVNDLRPNRDGTPPVINTDTPLVKKAREGVMEFLLINH
                     PLDCPICDQGGECDLQDQSMYYGFDGSRYKENKRAVEEKYLGPLIKTFMTRCIQCTRC
                     VRFMTEVAGVRELGAIGRGEDMEITTYLEQGLQSEMSGNIVDLCPVGALTSKPYSFVA
                     RPWELRKTESIDVMDGLGSNIRVDSRGREVLRILPRNNDGVNEEWISDKTRAVVDGLK
                     KKRLDRPYMRRGGKLEEVSWYEAFNEIAARVKKGGGRKVGAIAGDLCAVEEMFALKTL
                     LDELGSPHRDCRQLGAKLNPKNGRGSYLFNSTIAGVDEADAVLLIGTNPRKEAAVLNT
                     RLRKAWLHSRAAFALIGPEADLTYPYQWLGNSLKAIDDVARDGSPFLKVLSGASRPLI
                     IVGQAALNHAQGEAILSKAAEIATKAMKGKENSGWHPLSILHSSAALVGGLDIGFVPG
                     EGGLDVAGMADGAASGDIEVLYLLGADEFDVSRTGDAFVIYQGSHGDSGARIADIVLP
                     GAAYTEKNGTYVNTEGRVQLGERAIFPPGDAREDWTIIRALSDHLGARLPFDSLRDLR
                     TAMYKAYPHLAAVDVLQPENYTASPPNRLPPLPDEPLPELVDDYYQTNPIARASSVLA
                     ELSAISQATEHGATGTHG"
BASE COUNT          496 a          685 c          749 g          443 t
ORIGIN      
        1 cgccgcgccg cgacttgttt agaaaatcgg tcgaaatggc cacttcatgt gcggatacgc
       61 atagttaagc gttgctccac gccacgcctt aatgacataa gagcaccggg gatgaggcgc
      121 acaaaggata aaaatgccga agctgaacat caacggcacg gtgatcgagg tcgaggacgg
      181 gattacgctg ttgcaagcgt gtgagcaggc gggcgccgaa atcccgcgct tctgctatca
      241 cgagcgcctg tccatcgccg ggaattgccg gatgtgcctc gttgagatcg gcggcatgcc
      301 aaagccgatg gcctcctgcg ctatgagtgt caacgatttg aggccgaacc gtgacggcac
      361 ccctcccgtc atcaatacgg acacgccgct cgtaaagaaa gcgcgcgaag gggtgatgga
      421 gttcttgctc atcaaccacc cgctggattg cccgatctgc gaccagggtg gcgagtgcga
      481 tctgcaagac caatccatgt attacggctt cgacgggagc cgttacaaag agaacaagcg
      541 cgccgtcgaa gagaagtatc tcgggccgct gatcaagacc ttcatgacgc gctgcatcca
      601 gtgcacacgc tgcgtccgtt tcatgaccga ggtcgcgggc gtgcgcgagc ttggtgctat
      661 cggccgcggc gaagacatgg agatcaccac ctatctggag caggggctgc aatcggaaat
      721 gtcgggcaat atcgtcgatc tctgtccggt cggcgcactg acgtcgaagc cctattcctt
      781 cgttgcgcgt ccctgggagt tgcgcaaaac agagtccatc gacgtgatgg atggcctcgg
      841 ctcgaacatt cgtgtcgaca gccgcggccg cgaggtcttg cgcatcctgc cgcgcaacaa
      901 cgacggcgtg aatgaagagt ggatctcgga caaaacgcgc gccgttgtcg atgggctcaa
      961 gaaaaagcgg ctcgaccgcc cctatatgag gcgcggcggc aagctggaag aggtctcctg
     1021 gtacgaggct ttcaacgaga ttgcggcgcg ggtcaaaaag ggcggcggac ggaaagtcgg
     1081 cgcgattgct ggcgaccttt gcgcggtcga ggagatgttc gccctgaaga cgctcctcga
     1141 tgagcttggt tcaccgcatc gcgattgccg tcagcttgga gcgaagctca atccgaagaa
     1201 cggacgcggc agctatcttt tcaattcgac catcgcaggt gtggatgagg ccgatgcggt
     1261 gctcctgatc ggcaccaatc cccgaaaaga ggcggcggtc ctcaatacgc ggctcaggaa
     1321 ggcgtggctg cacagtcgcg ccgcattcgc cttgatcggg cctgaagccg acttgaccta
     1381 tccttaccag tggcttggaa actcgctgaa ggcgatcgac gacgtcgcgc gcgacgggag
     1441 cccgttcctg aaagtcctga gcggagcatc gcgaccgctt attatagtgg gccaagcggc
     1501 actcaatcac gcgcagggcg aagcaatcct gtcgaaggcc gccgaaatcg cgaccaaggc
     1561 gatgaagggc aaagagaatt cgggctggca ccccctcagc atccttcatt ccagcgcagc
     1621 gcttgtgggg gggctcgaca tcgggttcgt gccgggcgaa ggcggactgg atgttgccgg
     1681 gatggcggat ggcgcggcaa gcggcgacat cgaagtgctg tacctccttg gcgcggatga
     1741 attcgatgtg agccggacgg gagatgcttt cgtgatttat cagggctcac atggcgacag
     1801 cggcgcacgc atcgccgata tcgttctgcc gggagccgct tacaccgaga aaaacgggac
     1861 ttacgtcaat accgaaggcc gcgttcagct tggcgagcga gccatctttc cgcccggcga
     1921 cgcgcgcgaa gattggacaa tcatcagagc gctgtctgat catctaggcg cgcggctgcc
     1981 cttcgacagc ttgcgagacc tgcgaaccgc gatgtacaag gcgtatccgc atctggcagc
     2041 agtagacgtg cttcagccgg agaactacac ggcgagccca ccgaaccggt tgccgcctct
     2101 ccctgacgag cctctgccgg agcttgtgga tgactactac cagactaacc cgatcgcgcg
     2161 ggcgtcgtcc gtgttggccg agctttcggc gataagtcag gcaacagagc acggggccac
     2221 aggcactcat ggatagcttg agcatattct ggggcaagta tggcttcccg acggcgctca
     2281 ttctggggca aagcctgctg acgctcgtcg cgttgctcct gttcattgcc tacatccttc
     2341 ttgccgaccg gaagatttgg gcggccgtcc agt
//
LOCUS       DATMGX010000143         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_273584, whole genome shotgun sequence.
ACCESSION   DATMGX010000143 DATMGX010000000
VERSION     DATMGX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273584"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            553..1944
                     /locus_tag="VNR65_02360"
     CDS             553..1944
                     /locus_tag="VNR65_02360"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ17547.1"
                     /translation="MSPIRVPALDAKQPELMASYECAARSKQALHDIVIVGGGAGGLE
                     LATRLGKTLGKRKLARITLVEKSRTHLWKPLLHAVAAGSLDPSEEELNYLAQAHWRHF
                     DYQFGEMAGLNRAAKEIRLAATYDEEGREITPVRTVRYDTLVMAVGSVTNDFGTKGVA
                     QYAISLETAEHAVRFNRRLVNACIRAQTQTHPIRPGQLHVAIIGAGATGTELAAELHR
                     TAREVVAHGLNRIDPDRDIKITLIEAAPRILPALPEAVSDAAHQLLKKIGVDVHTGEG
                     VSEVSAHGVRLATGKFIPAELVVWSAGVKAPDFLKNLDSLETNKINQLVVNQTLQTTF
                     DPNIFAIGDCAACPRPGFSSLVPPRAQAAHQQASHLARQIRRHLKYEPLRPFRYKDFG
                     ALVSFGKYSTFGSLMGFHVGRNLFVEGYFARIIYRSLHLMHEVALYGSGRAWLRSLAR
                     ILSKPSEPTVKLH"
BASE COUNT          471 a          593 c          584 g          443 t
ORIGIN      
        1 agtgcatcgt aatgaggtta gttgaacttc agacaggaga tgccagatgg caatgcttca
       61 caaatttact ctcagggaat ggactgcttt cgctttggcc acggtcataa tgcttgctcc
      121 gttggcgcca tacgcattcg cgacctaaca cgccggagac actgtcgctt aggctgaaaa
      181 aagtgaagtg catatccaca aggccgcaga ggtgtggtaa cctagacctt tgcggcctta
      241 tggaatttat agcgcgtaaa gcgggactgc ggaggcatgg ccgcgatgag gtaaaggacc
      301 acggggccat ctttccgggc ttcggcgagc gggaatctga actccttgcg ctctgcgccg
      361 ccttcacggt gacttactca gaccagcaaa gatatagtct cagagacatg ctcaacttcg
      421 aggtctgagc aagtccactt gctgaaaaag ccccagattt cgttttttcc gcgtgacgag
      481 aaaaaagtgt ttgcgccttt agctgagtgg aaagtcatgc caccacaaat gaatattgcc
      541 tttcgagatg aagtgtcccc tattcgagtt ccggccctcg atgcaaagca gccggaactc
      601 atggcgtcgt atgaatgcgc ggcgaggagc aagcaagccc tccatgatat tgtgatcgtt
      661 ggcggcggcg caggaggcct tgaacttgcg acgaggctcg ggaagacgct aggcaagcgc
      721 aaactcgctc gcattactct ggtcgaaaaa tcccgcacgc atctttggaa accgcttctt
      781 cacgccgttg ctgcgggaag cctggacccg agcgaggaag aactgaacta tcttgcccaa
      841 gcacattggc gccacttcga ttatcagttc ggcgaaatgg ccggcctcaa ccgcgccgca
      901 aaggaaatcc ggctcgccgc cacctacgac gaagaggggc gtgaaattac gccggttcga
      961 acggtgcgtt acgacaccct ggtcatggcc gttggcagcg tcaccaatga tttcgggaca
     1021 aaaggggttg ctcagtatgc aatcagcctg gaaacggccg agcatgctgt ccgcttcaac
     1081 cggcgccttg tgaatgcctg tatccgcgcc cagacgcaaa cgcatcccat acggcccggc
     1141 cagttgcacg tcgcgattat cggggcggga gccaccggga cagaacttgc cgcagagctt
     1201 catcgcaccg cacgcgaagt tgtcgctcac ggactgaacc gcattgaccc tgaccgtgat
     1261 atcaagatca cactgataga agccgcgccc cgcattcttc ccgcgcttcc agaagccgtg
     1321 tcagatgccg cgcaccagct gctcaaaaag atcggggtgg atgtgcacac gggcgagggg
     1381 gttagcgagg tctcagctca tggagttcgc ctggcgaccg gcaagtttat acccgcggaa
     1441 cttgttgtgt ggtcggcggg cgttaaggcg ccagatttcc tcaagaacct cgatagtctg
     1501 gaaaccaata agatcaacca actggtcgta aatcagacgc tccaaaccac gttcgacccc
     1561 aatatcttcg ccatcggcga ctgcgcggcc tgcccgcgcc ccggattttc cagcctagtg
     1621 ccgccgcgcg cccaggccgc acatcagcaa gcgtcccatc tcgcaaggca gatccgccgc
     1681 catctgaagt acgaaccctt gaggcccttc cggtataagg actttggtgc ccttgtatct
     1741 ttcggcaaat attctacgtt cggcagtctg atgggctttc acgtgggccg caaccttttc
     1801 gtcgaaggct atttcgcgcg gatcatctac cgctcgctgc atttgatgca cgaggtcgca
     1861 ctatatggat cggggcgtgc gtggcttcga agtctcgctc ggattctttc taagccttcc
     1921 gagccgacag ttaagcttca ctgataaggt cgcgaaaggc tcaccttcga tcagatcgga
     1981 aaacggttgg tcccgaccga ggccggtcat aagttgctcg gataagctgc cttgccgaaa
     2041 ttggcatgaa atgcctggcg tccgaaggag gctcggcagt tttttagaag a
//
LOCUS       DATMGX010000144         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_125831, whole genome shotgun sequence.
ACCESSION   DATMGX010000144 DATMGX010000000
VERSION     DATMGX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125831"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..957
                     /locus_tag="VNR65_02365"
     CDS             49..957
                     /locus_tag="VNR65_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17548.1"
                     /translation="MATLREHGAMDYTVVVVTEGNDPPGLAFIAPYAATSIAEHFMNQ
                     GRDVLVVYDDLTHHARAYRELSLLLRRPPGREAFPGDIFYIHARLLERATHLRQETGG
                     GSLTALPIIETEAQNISAYIPTNLISITDGQIYLSPSLFELGVLPAVDVGKSVSRVGG
                     KAQRVAYRAVAGDLKLAYAQFEELETFARFGARLDENTRKIIEHGRRIRACLKQAENA
                     PVSVPAQIAVLLALTAALFDKVALDQMPDAEDAVRKAAANIPAEVCGRFESADNLSDE
                     DRNTIIEIARQALAPFQPKPDAEDKS"
     gene            954..1844
                     /locus_tag="VNR65_02370"
     CDS             954..1844
                     /locus_tag="VNR65_02370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HWJ17549.1"
                     /translation="MSDTMESLRRKISSAGDLQAVVRTMKAFAASSIGQYENSVRALA
                     GYYRTVELGLCACFREDTPAATADQRQAPTHAGEVRAIVFGSDQGLVGQFNDVVADYA
                     IKSLAALHGKCRVWAVGERVHARLSQQGLPLAGLFPVPNSVQAIAPLVGQIQIESEVH
                     RDKGRQAPLWVFHNRPLSGAIYEPVSQRLLPLDIQWRRHLATIHWPTANLPEVMGDGI
                     ETLRTLIREYLFISLFRACAESLASENASRLAAMQRADKNINDLLGQLNGTFHQLRQS
                     GIDEELFDVISGFEALRGSR"
BASE COUNT          449 a          631 c          614 g          435 t
ORIGIN      
        1 tgcgtttatt gcgccatcgg tcagcgcgcg tccgccgtcg cgaaagccgt ggcaaccttg
       61 cgggaacacg gtgcgatgga ttacaccgtc gtcgtcgtga ccgagggaaa cgacccgccc
      121 ggcctcgctt tcatcgcccc ctatgccgcg accagcatcg cggagcattt catgaaccaa
      181 ggccgcgacg tgctggtcgt ttatgatgac ctcacgcatc atgcgcgcgc ctaccgcgaa
      241 ctgtccctgt tgctgcggcg ccctcccggc cgtgaagcat ttcccggcga catcttttac
      301 attcacgcac ggctgcttga acgggcgacg cacttgcgcc aggaaactgg cggcgggtcc
      361 ctcaccgcgc tgcccattat cgaaaccgaa gcgcagaaca tttccgccta cattccaacg
      421 aacttgattt ccatcacgga tggccagatt tatctctcac cgtcattatt tgaattgggc
      481 gtattgccgg cagtcgatgt cggcaaatcc gtttcgcgcg tcggcggaaa ggcgcaacgc
      541 gtggcctacc gtgccgtggc gggcgatctc aaacttgcct acgctcagtt tgaggaattg
      601 gagacttttg cgcggttcgg cgcccggctg gatgaaaaca cccgcaagat catcgagcac
      661 ggacgtcgca tccgcgcctg cctcaaacag gcggaaaacg cgccagtgtc cgtacccgca
      721 caaatcgccg tcttgctggc attaaccgcg gcactattcg acaaggtggc gctcgaccag
      781 atgccggacg cggaggacgc agtacgaaag gcggcggcaa acatcccggc ggaagtgtgc
      841 ggacgatttg aaagtgccga caatttaagc gatgaagatc gcaatacgat cattgaaatc
      901 gcccgccagg cactggcccc cttccaaccc aagccggacg ccgaggacaa gtcatgagcg
      961 ataccatgga gagcttgcgc cgcaaaatca gcagcgccgg cgatctccaa gccgtcgtcc
     1021 gcacgatgaa ggcctttgcc gcctcgagta ttggacaata cgagaattcg gtgcgcgcat
     1081 tggccggcta ttatcggacc gtggaactgg gattgtgcgc gtgctttcgg gaagatacgc
     1141 cggccgcgac tgcggaccaa cggcaggcac caacccatgc cggtgaggta cgcgcaatcg
     1201 ttttcggatc cgatcagggg ctcgtggggc agttcaacga tgtggtggcc gattatgcga
     1261 ttaagagcct cgcggctttg cacgggaaat gtcgtgtctg ggcggtcggc gagcgtgttc
     1321 acgcgcgtct ctcacaacag ggcctgccgc ttgccggcct cttcccggtg cccaactccg
     1381 ttcaggcaat tgctcctctc gttggtcaga ttcaaattga aagtgaagtg catcgggaca
     1441 aaggccggca agcgcctcta tgggtgtttc ataaccgacc gctgtctgga gcgatctacg
     1501 aacccgttag ccagcggttg ttgccactcg atatacaatg gcgacgccat ttggccacga
     1561 tccactggcc taccgccaac ctgcccgagg tcatgggcga tggcatcgag actctgcgca
     1621 cattgatccg cgaatatctt ttcatctcgc tcttccgggc ttgtgccgaa tccctggcca
     1681 gcgaaaatgc gagccgcctg gcggcaatgc aacgcgccga caagaacatc aacgacctgc
     1741 tgggacagct taatggcact ttccaccagt tgcgccaaag tggaattgac gaagaactct
     1801 tcgacgtgat ctccggattc gaggcgctgc gcggatcacg gtagcaagct caaccttggg
     1861 agccagaata aaaatattct gcgggaaaac tgctgtgccg tttccgtcta gcgctctcgc
     1921 ttcacctgta tgatcctcgg cagttacgac tccgttgaat aagtaggcaa atgaagattt
     1981 ggctcagcaa acagttcgaa ttggaggaat ggcggcggag aatggtgttc tggggcgggg
     2041 ccgttgccac tggagcggac cccttacgcg ccgcgttgga acgattttga gctgggtttc
     2101 aataataatc gcttcaaaag gctctcatc
//
LOCUS       DATMGX010000145         2362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_15410, whole genome shotgun sequence.
ACCESSION   DATMGX010000145 DATMGX010000000
VERSION     DATMGX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15410"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1193
                     /locus_tag="VNR65_02375"
     CDS             180..1193
                     /locus_tag="VNR65_02375"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HWJ17550.1"
                     /translation="MSWGDIFFGFRGRINRKNYWTGSVLVGVAGFLFAGLLAYLATGN
                     PLAPEVWERPADKASIWGPVWLAYFGFLVWPSTALAVKRLHDRARPAWTWYVYYTLAL
                     AVSLVPLKASLGADTDATSGFLAVPLAVFAGYIFFELGVFRGTPGPNAHGEDPLPPDY
                     YGGDYNFWSWMFALEGRISRSKWWMGLFVLVSLIIAVSFVTGLMISSFLAAHPELQQH
                     LNSPEWFNSKEAEPVISSLGLWMIGPVLVMALVMWSLLALGVKRLHDRGLSSWLILVV
                     VLPFFGAVVAPSIAAEFDLGENIVRLSLLLLTASVIWSVLQFGILKGETGPNDHGPDP
                     LAG"
     gene            1200..1676
                     /locus_tag="VNR65_02380"
     CDS             1200..1676
                     /locus_tag="VNR65_02380"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HWJ17551.1"
                     /translation="MDRFLFIWLHLKGRIDRRTWLMFAVLIALSEYMAELILRRAFHW
                     PLPAASPGSPLLPGYLGDEISLLTALIFLWPSLAIDIKRWHDLGMRGWMVLLVYVPAV
                     VFHGLGFKGLGGTIDHPDQRVAAFLYLFGLAGLVYFIILAARKGMAGPNRYGPPPV"
     gene            1841..>2362
                     /locus_tag="VNR65_02385"
     CDS             1841..>2362
                     /locus_tag="VNR65_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWJ17552.1"
                     /translation="MVLQFLDADHFAGGYDGQERQSCQPFTTREVTIMTSALSHAQNL
                     IRCRSVTPDEGGALGYLETQLNAVGFTCHRLPFSERNTPDVDNLYARIGDGSPHVCFA
                     GHVDVVAPGDEAAWRHKPFAAEVDDGILYGRGAVDMKGAVAASLAAAQLFLLERGGEL
                     NGSISFLITADEEG"
BASE COUNT          416 a          687 c          682 g          577 t
ORIGIN      
        1 ggtgcgatgc ccggcaagcc gctcccgaac ggcgaagcag gcccgtcgct ctactgtgcc
       61 gtgatcgtga aacgcgtcga cggacaaacg cgcgccaaga cttcgattaa tgaactgttg
      121 cgcgactgac acggttgaat aaccgaacac atgttatcca gcccgtgatg gaggagcaca
      181 tgagctgggg cgatatcttc ttcggtttcc gagggcgcat aaatcgcaag aattactgga
      241 cgggaagcgt gctcgtcggt gtcgcgggtt ttctcttcgc cggtctcctt gcgtatctcg
      301 cgaccggaaa tccgcttgct cccgaggtct gggagaggcc cgcggacaag gccagcatct
      361 ggggcccggt ctggctcgcc tattttggct ttcttgtctg gccatcgacg gcgctcgcag
      421 tgaaacgcct ccatgaccgc gcacgccctg cctggacctg gtacgtttat tatacgcttg
      481 cgcttgctgt ttcgctcgta ccgctcaagg cgtcactcgg ggccgacacc gacgccacat
      541 ccggctttct cgctgtgccg cttgcagtct tcgccgggta tatattcttc gagcttggtg
      601 tgtttcgcgg tacccctggg ccgaatgccc acggagaaga ccccctcccg cccgactatt
      661 acggcggcga ttacaatttc tggagctgga tgtttgcgct tgaggggcgc atcagccgct
      721 caaagtggtg gatgggtctt tttgtccttg taagccttat tatcgcggtt tccttcgtga
      781 cgggcctcat gatcagcagc ttcctggccg ctcatcctga gcttcagcag cacttgaaca
      841 gcccggaatg gttcaacagc aaggaagcgg agcccgttat ctcgagcctt ggcctttgga
      901 tgattggccc ggtgcttgtg atggcccttg tgatgtggag cctgcttgcg ctcggtgtga
      961 agcggctcca tgatcgagga ctgagcagct ggctcattct ggtcgtggtc cttccattct
     1021 ttggcgcagt cgtcgctccc tcgatcgcag cggaattcga tcttggcgaa aatatcgttc
     1081 gcttgtctct cctgcttctg accgcatccg tgatctggag cgtcctacaa ttcggcatct
     1141 tgaagggcga aacgggtccc aacgaccatg gccccgatcc cctcgctggc tagcaattca
     1201 tggatcgttt tcttttcatt tggctgcatc tgaagggccg catcgatcgg cggacgtggc
     1261 ttatgtttgc ggttttgatc gcgctgtccg agtacatggc cgagcttatc ctgcggcgcg
     1321 ctttccactg gccactccca gctgcttctc cggggagccc gcttttgccc ggatacctcg
     1381 gtgacgagat ttcgctcttg accgcgctga tcttcctctg gccgtcgctt gctatcgaca
     1441 tcaagcgctg gcacgacctc gggatgagag gctggatggt gcttttagtc tacgttccgg
     1501 ccgtcgtttt ccacggtctg ggatttaaag gccttggcgg tacgatcgat catccggatc
     1561 agcgggtggc cgctttcctg tatctcttcg gccttgccgg tctcgtctat ttcatcatcc
     1621 tggcggcaag gaagggcatg gcaggaccga atcggtacgg cccgcctcct gtatgaacgg
     1681 cgctgcgcac acaccaacag cggcatccat tccaacattc agaagggcag ggaacgcatt
     1741 cactgctttg cttattgtgt gcagaaggtc tttttctgga atttccccgc gaaccacgcg
     1801 ttctcaatag agaaggcaaa taactttttc ccgggatagc atggttctac aattcctcga
     1861 tgcagatcat tttgctggag gttatgacgg acaggaacgg caatcctgcc agccattcac
     1921 cacccgtgag gtcacgatca tgaccagcgc attatcccac gcccagaatt tgattcgctg
     1981 ccggagcgtt acgccggatg aaggcggcgc gcttggctat ctggaaaccc agctcaacgc
     2041 cgtgggcttt acgtgccatc gcctgccttt cagcgagcgc aacacgccgg atgtcgataa
     2101 tctctacgcc cgcatcggcg atggctcacc ccatgtttgt tttgcggggc acgtcgatgt
     2161 cgtggctcct ggcgatgaag cagcttggcg ccataagccc tttgccgccg aagttgatga
     2221 cggcatcctc tatggaagag gcgccgtgga tatgaaaggc gcggtcgcgg cgtcgctggc
     2281 agctgcacag ctctttctct tggagagggg cggtgagcta aacggatcga tcagctttct
     2341 gataacagcg gacgaggaag gc
//
LOCUS       DATMGX010000146         5178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_442425, whole genome shotgun sequence.
ACCESSION   DATMGX010000146 DATMGX010000000
VERSION     DATMGX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442425"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VNR65_02390"
     CDS             complement(<1..669)
                     /locus_tag="VNR65_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ17553.1"
                     /translation="MTQRQNYLAEGFRNVDRQSDVEKFKTCLAFLDSLPSFRTYKEES
                     YRLLNPGEGSVFLDVGCGLGFDVERLARKTSAKIIGLDTSYELLNAARARAQDLGIAS
                     ADYVLADALLPCFKDCQFDGARVDRTLQHLDRPELVIKGMARTVRAGGRVVCAEPDWG
                     SFFVDDDDADCVHEVCREWTKSIKNPLIGRALPRLLKSAGLTNIKASGYLLATYGLRD
                     VNLVF"
     gene            complement(672..1892)
                     /locus_tag="VNR65_02395"
     CDS             complement(672..1892)
                     /locus_tag="VNR65_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HWJ17554.1"
                     /translation="MEKRSKSSLDATKVNAASFVIEEESLRHNLSVLKHVKDRTGCKM
                     FQALKTWSTWPLFHITKEYLDGTEVSSLNEARLGYDEFNHDVHMFAPAYSENEFPAIL
                     NTCSTIIFNTFAQWQRFKPHVNAHFRETGKTIECGLRINPEFIGKDEHGGLWSPCAPG
                     SRLGIRVSEFKKALAENPNALDGISGLHFHIFFDKVFEDLKEAIEVVEKNFGAYFSRM
                     KWINFGGGQKITDDDYDVEGLIMLVNDFQKRHGVTVHMEPGAAIVKNAGVLVATVLDI
                     VERDDVDFKIAILDMSFNAHMPDFLLSPDLDMHVTGAAIVRDAAEFEKHEHVYRLVGG
                     TCVSGDQLMQYHAFKQPLKPGDKVIMEDAIQYNLVQCTMFNGVQHPAIQLWTNGELKT
                     IREFAYEDYRARMG"
     gene            complement(2065..3291)
                     /locus_tag="VNR65_02400"
     CDS             complement(2065..3291)
                     /locus_tag="VNR65_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HWJ17555.1"
                     /translation="MTEGTVLIIGAGGVGSVTVHKCAMNANVFKKIHLASRTIAKPNA
                     IKASVKQRTGVDIATHTVDADETGQVIGLIENVKPEVVVNLALPYQDLAIMEACLATK
                     VPYIDTANYEHKDKAHFEYKQQWAFDGQYKNAGIMAVLGAGFDPGVTNAYCAYALKKY
                     FDKIRFVDIVDANAGSHEKAFATNFNPEINIREVTQVVRHFEHGRWVETPAILDKDAV
                     HFTFDFPVAGPKESYLLFHEEMESLAKNLPGLERMRFWMTFSENYLTHLRVIRNIGLD
                     NIKPIDFQGQKIIPMEFLKALLPDPASLATNYSGKTIIGCIITGVKDGKESTKFIYNV
                     CDHAETFKEVGSQAISYTAGVPPMVAAMMVMQGKWSGVGVFNVEQLDPEPFLEGVSAN
                     GLPFEVIDYKPLPAKI"
     gene            complement(3407..5134)
                     /locus_tag="VNR65_02405"
     CDS             complement(3407..5134)
                     /locus_tag="VNR65_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWJ17556.1"
                     /translation="MRLPIKLKLAANFTVLTALAAVVAWLGISSLGSLNATLSDIVQG
                     SVTRLQAADDLKLAVLDIVPFEKNMLMATSPEEIRVYEEELTKHRIAFLKKFESLQNI
                     ATNEVRQKLAALTPTWVRWTLVQDKMRELLDQNGQLEARALSLHEARDVKNEIVRQIE
                     EIDHIESQAMKQAREDAGKQYDHAWQLLAGTAGVALLVSIISGAILSLGISRGLRRAV
                     SLADAVSRGDLDQNVKATSNDEIKDLIDSLNRMTANLRATAEMAEAIAQGDLSVDPKP
                     LSEKDTLGLALREMTQKLRTVVSDALSAAGSVSSGSEELSSACQQLSAGANEQAASAE
                     EVSSSMEEMAANIKQNADNAAQTEKIARQSSADAQTSGDAVNRAVQAMQTIAEKITFV
                     QEIARQTDLLALNAAVEAARAGEHGKGFAVVASEVRKLAERSQTAATEISTLSGQTVT
                     AAREAGTMLVKLVPDIKKTAELVDEISAACREQDIGANQVNQAIQQLDKVIQQNAGAA
                     EEMSATSEALSGQAETLQTSIAFFRIGDAGHAGAAASAPAITVPAAIVKAPAKPKAEP
                     MRALPVSVY"
BASE COUNT          970 a         1521 c         1487 g         1200 t
ORIGIN      
        1 gaagaccaga ttgacgtcgc gcaacccata agttgccagc agatatccgc ttgccttgat
       61 atttgtaagc ccggccgact taagcaagcg aggcagcgca cggccgatga ggggattttt
      121 aatgcttttc gtccactcgc ggcagacctc gtgcacgcaa tcggcgtcat cgtcatcgac
      181 aaaaaagctg ccccaatcag gctcggcgca aacgaccctt cctcccgcgc gaacagttcg
      241 cgccatcccc ttgatgacga gttctgggcg gtcgaggtgc tgcaaggttc gatccacacg
      301 ggctccatcg aactgacaat ccttgaagca gggaagaaga gcatctgcga gcacataatc
      361 tgcacttgcg atgccaagat cttgtgctct cgcccgcgcc gcgttcagca attcatagct
      421 tgtgtcgagg ccaatgatct tcgcggaagt tttcctcgca agccgctcaa cgtcgaagcc
      481 gagaccgcag cccacatcaa ggaaaacgct gccttccccg ggatttaaga gccgatagct
      541 ttcttctttg tatgttctga agcttggaag agaatccagg aaggcgagac aggttttgaa
      601 cttctcgaca tccgactgcc ggtcgacatt ccggaagccc tccgccagat aattttggcg
      661 ctgagtcatg atcagcccat gcgcgcgcga tagtcttcgt aggcaaattc tcggatcgtt
      721 ttcagttcac cgttcgtcca tagctggatg gccggatgct ggacgccatt gaacatcgtg
      781 cattgtacga ggttatactg gatggcgtcc tccataatca ccttgtcgcc gggcttcagc
      841 ggctgcttga aagcgtgata ctgcatgagt tgatcgcctg agacgcaggt gccgccaacc
      901 aggcgataga cgtgttcgtg cttttcgaac tccgccgcat cgcgaacgat tgccgcgcct
      961 gtcacgtgca tatcgagatc agggcttaag agaaaatccg gcatgtgcgc attgaacgac
     1021 atgtccagga tcgcgatctt gaagtccacg tcgtcgcgct cgacgatgtc cagcacagtc
     1081 gcgaccagca cgcccgcgtt tttcacgatg gcggcgcccg gctccatgtg aacggtcacg
     1141 ccgtgccttt tctggaaatc gttgacgagc atgatcaagc cctcaacatc gtaatcatca
     1201 tcggtgatct tctgcccgcc gccgaagttg atccatttca tacgggaaaa atatgcgccg
     1261 aaatttttct cgacgacttc gatcgcttct ttcagatctt cgaagacctt gtcgaagaag
     1321 atgtggaagt gcagcccgct gatgccatca agcgcatttg gattctcggc cagcgccttt
     1381 ttgaactcgc tgacgcgaat gccgagccgc gatccgggag cgcacggcga ccatagtccg
     1441 ccgtgctcat cttttccgat gaattctgga ttgatgcgca agccgcactc gatggtcttg
     1501 cccgtttccc taaagtgggc attcacatgc ggcttgaacc tttgccactg cgcgaatgtg
     1561 ttgaagatga tcgtgctgca ggtgttgagg atcgcgggga attcgttctc actgtatgca
     1621 ggcgcaaaca tatgtacgtc atgattgaac tcgtcatagc cgaggcgcgc ttcgttcaat
     1681 gagcttacct cggttccgtc caagtactcc ttcgtgatat ggaaaagcgg ccatgtggac
     1741 caggttttca gcgcctggaa catcttgcag ccagttctgt ctttgacgtg cttcaggacg
     1801 ctcagattgt ggcgcagact ttcttcctcg atcacgaatg aggcggcgtt cacctttgtg
     1861 gcgtcgagcg aagacttgga ccgcttttcc atgcgctaag taaacttccg cttatctggc
     1921 caacggattg tcatcccgga aaggcgaggc cttatccggg atcgtcacga actaaagcgc
     1981 cttacttttt gcgatcccgg ctcgcgcttt gcttggccgg gatgacggcg gcgccgatac
     2041 gttggcgcaa ccggcaaaat ctttctaaat cttcgccggc aggggcttgt agtcgatcac
     2101 ctcaaacggc aggccgttcg cggatacgcc ttcaaggaag ggctcaggat cgagctgttc
     2161 gacattgaac acgcccacgc cgctccattt cccctgcatg accatcatgg ccgcaaccat
     2221 gggcggcacg cccgccgtgt acgaaatcgc ctgcgagccg acttccttga acgtctctgc
     2281 gtggtcgcaa acgttgtaga tgaacttcgt gctctccttg ccgtccttca cgccggtgat
     2341 gatgcagccg atgatcgtct tgccgctgta gttggtcgcc agcgaggccg ggtcgggcaa
     2401 cagcgctttc agaaactcca tcggaatgat cttctggccc tggaaatcga tcggcttgat
     2461 gttatcgagg ccgatattcc gaatgacacg caggtgcgta aggtagtttt ccgaaaacgt
     2521 catccagaag cgcatgcgct ccagccccgg aaggttcttg gcgagcgact ccatttcctc
     2581 atggaacaag agatagcttt ccttggggcc tgcgaccgga aagtcgaagg tgaaatgcac
     2641 ggcgtcttta tcgaggatcg ctggggtctc cacccacctg ccatgctcga aatgccggac
     2701 gacctgcgtg acttcgcgga tgttgatctc gggattgaag ttggtcgcaa aggctttttc
     2761 gtgtgagccc gcattcgcat ccacaatgtc gacaaagcgg attttatcga aatatttctt
     2821 cagcgcgtag gcgcaatagg cgttggtgac gccagggtcg aagccagcgc caagaaccgc
     2881 catgatcccc gcgttcttat actggccgtc aaaagcccat tgctgcttgt actcgaaatg
     2941 cgccttgtcc ttgtgctcat aatttgcggt atcgatgtaa ggcaccttcg tcgccaggca
     3001 cgcctccatg atcgcaaggt cctggtaggg gagggcaagg ttgacaacga cttccggttt
     3061 cacgttctcg atgagaccga tcacttgtcc tgtctcgtca gcgtcgacgg tatgggtggc
     3121 gatatcgacg ccggtgcgct gcttgaccga agccttgatc gcattcggct ttgcgatggt
     3181 gcggctcgcc aggtggatct ttttaaaaac attcgcgttc atggcgcatt tgtggaccgt
     3241 cacgcttccg acgccgcccg cgccgatgat gagcacagtt ccttcggtca tgagatttta
     3301 tccctcggct tctcagcttg acgagcaaga gcagttcggg aacctcataa caaaaaaagg
     3361 cccttgcgtc acattcatcg gcgctcacga tttggttttt gggtgctcaa tagacgctca
     3421 ccggtaaggc tcgcatcggc tccgctttcg gcttcgccgg cgcctttacg atcgccgcgg
     3481 gcaccgtaat cgccggtgca gaggctgccg cacccgcgtg gccggcatcg ccgatccgga
     3541 aaaacgcgat gctcgtctgc agcgtctcgg cctggcctga gagcgcctcc gaagtcgccg
     3601 acatctcctc ggctgcaccc gcattctgct ggatcacctt gtcgagctgc tggatggctt
     3661 ggttcacctg attggcgccg atatcctgct cgcggcaggc cgcgctgatc tcgtccacaa
     3721 gctccgccgt cttcttgatg tcgggcacga gcttcacaag catcgtgccg gcctcgcgcg
     3781 ccgccgttac ggtctggcct gacagcgtgc tgatctcggt ggctgccgtc tggctccgct
     3841 cggcgagctt gcgcacctcg gatgcgacca ccgcaaagcc cttgccgtgc tcgcccgccc
     3901 gcgccgcctc gacggccgcg ttcagcgcaa gaaggtcggt ctgccgcgcg atctcctgca
     3961 cgaaggtgat cttctcggca atcgtctgca tggcctgcac tgcacggttc acggcgtctc
     4021 cgctcgtctg cgcatcggcc gatgactggc gcgctatctt ctcggtctgg gccgcgttgt
     4081 cggcgttctg cttgatgttg gcggccatct cttccatgga cgaggagact tcctcggcgg
     4141 atgccgcctg ctcattggcg ccggcggaga gctgctggca ggcggaggaa agttcttcgc
     4201 tgccggaaga gacgctgccc gcggcgctca aggcatcgct gacgacggtg cgaagcttct
     4261 gggtcatctc ccggagcgca aggccgagcg tgtccttctc cgacaggggc ttcgggtcaa
     4321 cggagaggtc gccctgggcg atggcctcgg ccatctcggc ggtggcgcgc aagttggcgg
     4381 tcatgcggtt cagcgagtcg atgaggtcct tgatttcgtc gttgctcgtg gctttgacgt
     4441 tctgatcgag atcgccgcgc gagaccgcgt cggcgagcga gacggcgcgg cgcaaaccgc
     4501 ggctgattcc gagtgatagg atggcgccgg agatgatgct gaccaggagc gcgaccccgg
     4561 ccgtcccggc gagcagctgc caggcatggt cgtattgctt gcccgcatcc tccctggcct
     4621 gcttcatcgc ttggctctca atgtgatcga tctcctcaat ctgccgcact atttcgttct
     4681 tgacgtcgcg ggcttcatga agtgacaggg ccctcgcttc cagttggcca ttctggtcaa
     4741 gaagctcgcg cattttgtcc tgcactaagg tccagcgcac ccatgtgggt gtcaaggcgg
     4801 ccaatttctg ccttacctcg ttggttgcga tgttctgcag gctttcgaac ttcttaagga
     4861 aagcgatgcg gtgtttcgtg agttcttcct catagacccg gatctcctcc ggcgaggtcg
     4921 ccatgagcat gttcttctcg aacggcacga tatccaggac cgccagcttg aggtcgtcgg
     4981 cggcctgaag acgcgtcacg ctgccctgaa caatgtcgct gagagtggcg ttgagcgatc
     5041 caaggcttga gatgccgagc caggccacga cggctgccag cgcggtaaga acggtgaagt
     5101 tcgcggcgag tttaagcttt atggggaggc gcatctttgt tgtccttcgt tattggaggt
     5161 ccatcaggcg caatgcgg
//
LOCUS       DATMGX010000147         5021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_27115, whole genome shotgun sequence.
ACCESSION   DATMGX010000147 DATMGX010000000
VERSION     DATMGX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27115"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VNR65_02410"
     CDS             complement(<1..1341)
                     /locus_tag="VNR65_02410"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HWJ17557.1"
                     /translation="MNREVPAVNKTWAELKPGDESTLRRLCVADDFIVFAHASGNRNP
                     IHLSGKHGAPAPAMWVGALVSSVLGNVLPGPGTIYHDQSLHFHGPAHDGDELVIGVRV
                     KEKRAEPEVVLETWVDRADGVRLCDGEAIVAAPTARVTFSTAEVPGLIVRRHRHFEAL
                     LRRAEQLPPLLTAVVAPEEPHSLGGALLAARENIITPILIGDRARIEAAAKQLGESLG
                     SAQIVPIASHSAAAARAVAMVHEGTAQAVMKGHLHTDELLRHVVKREGGLRTERRISH
                     VFVMDVPGLDHPLLISDSAINIAPDLEAKVDIVQNAIDLARAIGIAEPRVAVLSAVET
                     VTPKIPSTVDAAALAKMSERGQIEGGIVDGPLAMDNAMSLEAAQTKGIKSFVAGRAEV
                     LIAPNLEAANMIVKQLTFVAHAEGGGLVLGASAPVILTSRADDERVRLGSCAVAA"
     gene            complement(1338..1526)
                     /locus_tag="VNR65_02415"
     CDS             complement(1338..1526)
                     /locus_tag="VNR65_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17558.1"
                     /translation="MGTRPSLSAFRNPFITPITDSNAWIENELALADCIANADTSRLL
                     AVAGLLKNPLVQLMEATL"
     gene            1701..2003
                     /locus_tag="VNR65_02420"
     CDS             1701..2003
                     /locus_tag="VNR65_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17559.1"
                     /translation="MPFWVGHACLEVLQRLQLLEEGLGPIARKNGGEELSHQRAFFKE
                     RRTLCLVLLFRFKIVDRPPAFCDLFPSLPECLARAVLKLEKTGLPQASARRFSRYP"
     gene            2134..3798
                     /locus_tag="VNR65_02425"
     CDS             2134..3798
                     /locus_tag="VNR65_02425"
                     /inference="COORDINATES: protein motif:HMM:NF027688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase family protein"
                     /protein_id="HWJ17560.1"
                     /translation="MKGAAALALGLASDAQAEAPQSIPGSGASAAGTVSGIVYESSTG
                     EPHRAGLPGVAGVLVSNGREVVRTDAQGRYTLPIDDEAVIFVIKPSGYAVPTDPATNL
                     PRFYFIHQLKGTPADLKLRFRGIDPTGPLPESVDFPLIRQAEKTKFDVLLFTDPQPES
                     HVELDFVRDDVVNALVGSNAAFGITTGDILFDDLSMYGRYNRIIGQIGLPWWNIGGNH
                     DLNFEAKDRTYSRETYKQVFGAPYYAFHHGDALFLMLDNVTYLGPDPSRPADRGGKYI
                     GEFGSRQLAFVENVLKETPKDKLVVACMHIPLESYVNDDPANGTADRNDFLKLFAGRK
                     VLSLAGHTHTTEHHYFDIEGCWQHHHHVLTAVSGSWWSGPFDYRGIACALSRDGSPNG
                     HHVLSIDGTTYETRFVAAKEPQGRKTRIMLDSVFHRSGRELQRDFRLGQMLGSPISVD
                     TLHATEVIVNVFDGGPKTKVALTIGKAAPIAMTRDARPDPLASELFARNEATKKPWVK
                     AENSSHIWTARLPSDLEPGSHTLTVTVQDEYGRAFSERAVVEVVCA"
     gene            complement(3952..4305)
                     /locus_tag="VNR65_02430"
     CDS             complement(3952..4305)
                     /locus_tag="VNR65_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17561.1"
                     /translation="MPFAQIADIESLPKKAKLMKIWKTLVRVEGSAILAVDTIEYKGK
                     LWLVPERTTEHATGTQRPTRLIGLRFGLPLMKPGPNSAHLQLAISLNKETLAGRTAQG
                     FDVIEAPDVRWRHAA"
     gene            complement(4798..>5021)
                     /locus_tag="VNR65_02435"
     CDS             complement(4798..>5021)
                     /locus_tag="VNR65_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17562.1"
                     /translation="SVAPAGYVFPAGESAQMVQYNDKEHGFLRMTLDLKRRTLIGEYF
                     AAYNAASGSTGVLALRDSFTLDLSKHRVS"
BASE COUNT         1031 a         1481 c         1477 g         1032 t
ORIGIN      
        1 ggctgcgacg gcgcacgagc cgagacgcac ccgctcgtcg tcggcgcggc ttgtcaggat
       61 gacaggcgcg gaggcgccca gcacgaggcc gccgccttcc gcatgggcga cgaaggtcag
      121 ctgcttcacg atcatgttcg ccgcttcgag gttgggcgcg atgagcacct ccgcgcggcc
      181 cgccacgaag ctcttgatgc ctttggtttg cgcagcttcc aggctcatgg cgttgtccat
      241 ggcgagcggc ccgtcgacga taccgccttc gatctggccg cgttccgaca ttttggcgag
      301 agccgcggca tcgacggtgg acgggatctt cggggtcacc gtttccactg cggacagcac
      361 ggccacccgc ggctcggcaa tgccgattgc ccgggcgagg tcgatggcgt tctggacaat
      421 gtccaccttc gcctcgaggt ccggggcgat gttgatggcg gaatcggaaa tgagcagggg
      481 atggtcgagg ccgggcacgt ccatcacgaa gacatgggat atgcggcgct cagtacgcag
      541 cccgccctcc cgcttgacca cgtgtcgcaa gagctcgtcg gtatgaaggt gacctttcat
      601 cacggcctgc gcagtgccct catgcaccat ggccactgcg cgagcggcgg cggcagaatg
      661 ggaagcgatg gggacgattt gtgcgctgcc gaggctttcg cccagctgtt tggctgccgc
      721 ctcaatgcgg gcgcggtctc cgatgaggat gggggtgatg atgttttcgc gtgcggctag
      781 aagtgcgccg ccgagcgaat gcggctcctc cggcgccacc accgccgtca ggagcggcgg
      841 caactgctcg gcccggcgca gcagcgcctc gaaatggcgg tgccgccgca cgatcaggcc
      901 gggcacctca gcggtagaaa aagtgacgcg cgctgtgggg gcggccacga tggcttcgcc
      961 atcgcaaagg cgaacgccgt cggcgcggtc cacccaggtt tcgagaacca cctcgggctc
     1021 cgcccgcttt tccttcacgc gcacgccgat gacgagttcg tcgccatcgt gcgcaggccc
     1081 atggaaatgg agagattggt cgtgatagat ggtgccgggg ccaggcagga cgttgccgag
     1141 cacggaggaa acaagagctc ctacccacat ggcgggggcc ggcgcgccgt gcttgccgga
     1201 aagatggatg gggttgcggt tgcccgaggc gtgggcgaac acgatgaagt cgtcggccac
     1261 gcacagccgc cgcaacgtcg actcgtctcc gggcttgagt tctgcccatg ttttgttcac
     1321 agcagggact tccctgttca tagcgtggcc tccatgagct gaacgagagg attcttgagc
     1381 aaaccggcta ctgcgagcag tctgctagta tcggcgttgg cgatgcaatc ggcgagcgcg
     1441 agctcgttct cgatccaggc gttgctgtcg gtgatgggag taataaacgg gttccggaaa
     1501 gcagaaagcg aaggccgcgt gcccaaacca tcctccttcc gacccattag agatcggacg
     1561 aatacttccc cggtagcgaa atatgtccgg cggaacacgc cggaagcgtt aaggtcacgc
     1621 tatcaatctt agattacagt attgtggcac ttggatattg cggctaagcc aatatccagc
     1681 gcgtcgatct ggagtcctca atgcctttct gggttggcca cgcgtgcctt gaggtcttgc
     1741 agcgtcttca gctgcttgag gaagggcttg gccctattgc ccggaaaaat gggggcgaag
     1801 aactcagtca tcagcgcgcc ttcttcaagg agcggcgcac cttgtgtctt gtgcttctgt
     1861 tcaggttcaa gatagtcgat cgcccgcccg cattttgcga cctgtttcca tcgcttccag
     1921 agtgtcttgc tcgcgcagtc ctcaaactgg agaaaacagg cctgcctcaa gcatcggcgc
     1981 ggcggttcag ccggtaccct tagccaaagt gacacatccg tcttttaaaa tttattgcct
     2041 tcagcatcgc gacctgccgt cccacagctc tgcagaactc acacgaggac aaagccgcaa
     2101 atggaacgaa ccgcagtcac acgaagggat gcaatgaagg gcgctgcggc gctcgcactc
     2161 ggcttggcat cggacgcgca agctgaggcc cctcaaagca ttcctggctc cggcgcctca
     2221 gccgccggaa cagtcagcgg gatcgtttac gaaagcagca ccggcgagcc tcaccgtgcc
     2281 ggactacccg gtgtggcggg cgttcttgtc tcaaatggcc gggaggtcgt ccgaacggat
     2341 gcgcaaggcc gatacacgct tcccatcgat gacgaggcgg tgatctttgt catcaagccc
     2401 tcgggctatg ccgtgcccac cgatccagcc acaaacctcc cccgctttta cttcatccac
     2461 cagctaaaag gaacgccggc cgatcttaag cttcgcttcc gcggtatcga tcctaccgga
     2521 ccgctgccgg agtcggtcga tttcccgctt atccgacaag cggagaaaac caagttcgat
     2581 gtccttctct tcactgatcc gcagcccgaa agccatgtgg agcttgattt tgtccgcgac
     2641 gatgtcgtga acgcgcttgt tggctctaac gctgccttcg ggatcacgac cggcgatatt
     2701 ttgttcgacg atctttcgat gtatggccgc tacaatcgca tcatcgggca gatcggcctt
     2761 ccttggtgga atattggcgg caaccacgat ctgaacttcg aggccaagga ccgcacctat
     2821 tcccgcgaga cctacaagca ggtcttcggg gcgccttatt acgccttcca tcacggcgac
     2881 gcgctgttcc tcatgctcga caacgtcacc tatctcgggc cggatccgag ccgcccggcg
     2941 gatcgcggcg gcaaatacat cggagagttt ggcagcaggc agcttgcgtt cgtggaaaat
     3001 gtcttgaaag agactcccaa agacaagctc gtcgtggctt gcatgcatat cccgctcgaa
     3061 agttatgtca acgatgaccc ggccaacggt acggctgatc gcaatgactt tctaaagcta
     3121 ttcgcaggcc gcaaggtgtt gagccttgca ggccacactc acacaaccga gcatcactac
     3181 tttgatatcg agggctgctg gcaacatcac catcacgttc tgactgccgt ctccggctcc
     3241 tggtggagcg ggccctttga ctatcgcggc attgcgtgcg cgctgagccg cgacgggagc
     3301 ccgaacggcc accatgtcct cagcatcgac gggacaacct atgagacacg cttcgtcgcg
     3361 gcaaaggagc cgcagggacg gaagacgcgg atcatgctcg acagtgtctt tcaccgctcg
     3421 ggacgggagt tgcagcgcga tttccgcctg ggacagatgc tcggctcgcc aatttcagtc
     3481 gatactctcc acgcaaccga ggtcattgtg aacgttttcg acggcggtcc gaaaacgaaa
     3541 gtggccctta cgattggaaa ggctgcgccc atcgcgatga cgcgcgacgc gcgtccggat
     3601 ccccttgcga gcgagctttt tgcgcgaaac gaggccacga agaagccctg ggtcaaggca
     3661 gaaaactcat cccacatctg gaccgctaga cttccgagcg acctggaacc tggatcgcat
     3721 actctgacgg tcacagtgca ggatgagtat ggacgcgcat tttcggagcg cgctgttgtc
     3781 gaggtcgtct gcgcctgaac ggtgtgatcg atggttgccg gcgtttttcg aagcttagcc
     3841 ggacttatgg gatcggacag tgggaacggg agaagctata cggcggctgt gaccttggcg
     3901 gcaaacatcg tcagaaaact cggccttatg tagcaagcgc accaaactaa tttatgcggc
     3961 atggcgccag cgtacatccg gtgcctcaat cacgtcaaag ccttgggctg ttcggcctgc
     4021 aagggtttcc ttgtttaatg aaatcgctaa ttggagatga gccgaattcg gtcccggttt
     4081 catcagcggc aatccaaacc gcaaacctat tagtctcgtt ggcctttgcg tgcctgtagc
     4141 atgctctgtg gtccgttccg ggaccagcca gagcttgccc ttatattcga tggtgtcgac
     4201 ggcgagaatt gcactgcctt cgacgcgaac aagagttttc catatcttca tgagtttcgc
     4261 tttcttcggc aatgattcga tatccgcaat ttgagcgaat ggcaaagcct aagcttcccc
     4321 gcctcacctt ttcgactgat tttttgttta cactctctga tttatatagt taatttacac
     4381 aactaatgct tagttttctc acttactaca cgagtgtgca accctgtgat agagactaaa
     4441 cctctatcaa tcctgcgaga atgccatgaa gcaatgccaa tcttgcggaa taccgctttc
     4501 caaagacccc ggtggtggcg gaacaggaag agacggacgc aaaagccaga cctactgcag
     4561 ccggtgttac cagaatgggg aattcatcgc taaggactgc accgtcgaac aaatgcaaga
     4621 cattgttgag caagccatga aaaaggaagg ctacagctgg gccatcagga aaatggtccg
     4681 atttcagatc cctcaactcg cccgctggaa aaagcagtct taaccgacga tcgacgtctg
     4741 ctccctaacc ccgcattctt cggctgagcc gcaccacgca cccaaaggca tgcgcaatca
     4801 ggaaacgcgg tgcttgctta gatcgagcgt gaagctgtcg cgcaaggcga ggacaccggt
     4861 tgagccgcta gctgcgttat aggccgcgaa atactccccg atcagcgtgc ggcgtttgag
     4921 gtcgagcgtc atccgcagga atccgtgctc cttgtcgttg tactgcacca tctgggcgct
     4981 ttcgcctgcg ggaaagacgt atccggcagg cgcgacggag a
//
LOCUS       DATMGX010000148         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_247927, whole genome shotgun sequence.
ACCESSION   DATMGX010000148 DATMGX010000000
VERSION     DATMGX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247927"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VNR65_02440"
     CDS             <1..984
                     /locus_tag="VNR65_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17563.1"
                     /translation="EPTLTTDRQSVRYIGPTLVCLDLPSRVQPSGPHEMSECGDSVAM
                     LPAPSTVPQKPGLQHLAPQAYIATLPDYIQAEYPTGEVTYAVEVLNRNARGAGLSNRV
                     RVPAVFTLRPPGDLSAELTGDGVVLTWTSAGGLKRLPANSPSTPPSIQLRYRIYRRDE
                     GSGKDATAGEIPLGEPGPVHFTDTSFEWEKTYLYHITAVSIVKRRDSEVQVEGDDTPP
                     VRVIAHDVFPPAVPAGLQAAYSGEGQRSFIDLIWAPVTSADLAGYNVYRSETNGAETE
                     VMVKLNSEPAKSPSYRDLAVASGKSYTYSVSAVDVRGNESQRSEETSEPVP"
     gene            1103..>2125
                     /locus_tag="VNR65_02445"
     CDS             1103..>2125
                     /locus_tag="VNR65_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWJ17564.1"
                     /translation="MPDELLRYRSEFPILERTNYMISNSLGAMPRAVYGALHAYADAW
                     ATRGVRAWEERWWMLALEVGNELGTLMNAPRDSVSLHQNVTTCQAVVASCFDFSGRRN
                     KVVYSDMNFPSVMYFWEAQRQYGAQVHMVKTDDGVTVPTDRLLDAIDEQTLIVPISHV
                     IFRSSYINDAKAIIDKAHRVGAHVVLDTFQSLGTVPVDIQSLNADFACGGVLKWLCGG
                     PGVAYLYVRPDLGKKLEPKLTGWFAHENSFAFATGPIRYADPPFRFMNGTTHIPAIEA
                     ARPGLKIIQEVGVEKIREKSKHQTTRLIAFADQHGWRVNTPRDPEQRGGTVSIDMPDS
                     QQVSREL"
BASE COUNT          450 a          733 c          558 g          384 t
ORIGIN      
        1 gagcccacgc tcaccaccga ccgccaaagc gtgcgctaca tcggtccgac cctcgtctgc
       61 ctcgacctgc caagccgcgt tcagccctcc ggtcctcacg agatgagcga atgcggagat
      121 tccgtcgcga tgttgcccgc accatctacc gttccgcaga aaccgggcct tcagcacctc
      181 gcgccgcagg cctacatcgc gacacttccc gattacattc aagcagagta cccgaccggc
      241 gaggtcacct acgccgtcga ggtcttgaac cgcaacgccc gcggcgccgg attgtcgaat
      301 cgcgtgcgcg tgcccgccgt cttcaccttg cgtccgccgg gcgatctatc cgccgaactc
      361 acgggcgacg gcgtagtgct cacgtggaca agcgcaggcg gattaaagcg cctccccgct
      421 aactcgccca gcaccccccc cagcattcaa cttcgttatc gaatctatcg gcgcgacgaa
      481 ggctccggca aagacgcgac tgccggcgaa atcccattag gcgagcctgg ccccgttcac
      541 ttcaccgaca caagcttcga atgggaaaag acctatctct accacatcac cgccgtgagc
      601 atcgtcaaac gtcgcgacag cgaggtgcag gttgagggag acgacactcc gcccgtgcgc
      661 gtcatcgccc atgatgtctt cccgcccgct gtgcccgccg ggttgcaggc ggcatactca
      721 ggcgaggggc aaagatcatt tatcgatctc atttgggcgc cggtgacgag cgccgatctc
      781 gcgggataca acgtctatcg cagcgaaacg aatggcgccg aaaccgaggt catggtcaag
      841 ctgaattctg aaccggcgaa atcgccgtcg taccgcgacc tcgcggtcgc ctcgggcaaa
      901 tcctatacct actcagtttc tgcagtggac gtgcgtggaa acgaaagcca gcggtccgaa
      961 gaaaccagcg aacctgtccc ctagggtaac cctgactcag caggttccgg gatagctatg
     1021 cgaatggcga aagacggaaa gccaaaagct aagagctaag agcggagcag cgaaaagctg
     1081 tccccggatt acaatgacct tcatgcccga cgaacttctg cgctaccgct ccgagttccc
     1141 catcctcgag cgcaccaact atatgatctc caattccctg ggagccatgc cccgtgcagt
     1201 ttatggcgcg ctccatgcct acgccgacgc ctgggcaaca cgcggcgttc gcgcctggga
     1261 agaacgctgg tggatgctcg ccctcgaagt gggcaacgaa ctcggcacct tgatgaacgc
     1321 gcccagagat tccgtctctc tgcaccagaa cgtcactacc tgccaggccg tggtcgcctc
     1381 ctgttttgac ttttctggca ggcgcaacaa agtcgtttac agcgacatga actttccctc
     1441 cgtcatgtat ttctgggaag cgcagcgcca gtacggcgcg caggttcaca tggtcaagac
     1501 ggacgacggc gtcaccgtcc ccaccgatcg cctgcttgac gccatcgacg agcaaactct
     1561 gatcgttccc atctctcacg ttatttttcg cagctcctat atcaacgacg ccaaagccat
     1621 catcgacaaa gcccacagag tcggggccca cgtcgttctc gacacgttcc agtctctcgg
     1681 caccgtccct gtagacatcc agtctctaaa cgcggacttc gcctgcggag gcgtactcaa
     1741 gtggctatgc ggcggccctg gcgttgccta tctctacgtc cgccccgacc tcggcaagaa
     1801 attggagccg aagctcacgg gatggttcgc gcacgaaaat tcctttgcct tcgccaccgg
     1861 ccccatccgc tatgccgatc ctccgttccg attcatgaac ggaactaccc acattcctgc
     1921 catcgaggcg gcgcgtcccg gcctcaaaat cattcaggaa gtcggcgtgg agaaaatccg
     1981 cgagaaatcc aaacaccaga ccacccgcct catcgcgttc gccgaccagc acggatggcg
     2041 cgtcaacact ccccgcgatc ccgagcaacg cggcggcacc gtctccatcg acatgcccga
     2101 ttcccaacag gtgagccggg agctc
//
LOCUS       DATMGX010000149         2255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_247856, whole genome shotgun sequence.
ACCESSION   DATMGX010000149 DATMGX010000000
VERSION     DATMGX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247856"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1847..1975
                     /locus_tag="VNR65_02450"
     CDS             1847..1975
                     /locus_tag="VNR65_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17565.1"
                     /translation="MSRNFGVPRQTRDAYRVTTWSLVAVTLIMIGLINVAAHLSHW"
BASE COUNT          495 a          674 c          584 g          502 t
ORIGIN      
        1 ggaaggcgcg ccgttcgtac gtccacatac catgctctta gggcacatag gtgccctatg
       61 tattcaaggt ttctctgtaa acgagcatca gtgtcaccac gtattccaaa gtcccacgga
      121 gcatcaaccc gccgtggcag gtcagagtgg atcctatggt ccgcgatatc cgaccgaatg
      181 atctgcccgg cgacactgaa ggatccgaat ccaacctgga gtggcccaac catgccgctg
      241 agaccaccaa taaaaatccg ttcctctctt aagaacactc cacgaggaac tcctccgatc
      301 agagagggcg ttgccttatc tccgctctct ccccaagggg tgaaattaaa atggatgaaa
      361 ccgctgccta cctcagtgtg gttgcttcga gagcgccccc ccgatatcaa acaatcgcag
      421 cagtttatga gtgatcctaa ggtcacgaag catgttagga ttgtttgctt caggccgacg
      481 gcgtgcgcca cggaagcccc ttcttccaga agcgtgcccg acctgatgtg accatttgct
      541 ccgatccttg ctccgtccag aagaacagag cctgttacaa acccgcttgc tacttccgcg
      601 ctcgctccga taaccgagtc gacgattgtt gccggcccct cacttccgat tttcgctcct
      661 ggcgcaatta ccgtgttctt ccccgagaga cgcgttccgg gataaaggac cgactccgga
      721 aagatctgtt caagcacgac gtcgtcttct acgtaaactt gacgaggatc gatgagttga
      781 actcctcggt cggcaaggag ccgtagtcgc tcggatatct tcatcgcctt catagagctc
      841 actcaaatgg atggagatgt cttgttaccc caattgcccc aaaaacctga catcgcgatc
      901 atttctaact tccccggttg tattcgcgag caacggtacc ggaaagaacg ctatctcttg
      961 cgacgatcgc gccttaggca caatcgaccc cgctcgtacg ttggcgctgt ctcccacgaa
     1021 agctcccgca tcccccattg actgggattc tcccaccaca gcgcccgcgc cgacaaacgc
     1081 gccggcgccc aaaacggctt tctatatggc atagttttcg gagatgattg aaccattcat
     1141 tggaacgctt tgagatacca ttgcgcccgg acccaccctc gaaccggcac ccatgaagct
     1201 gccgtccagg accttagcgt cgcaatcacc gccccccgac cgttgagagg taacccgctc
     1261 cttcagaatt gggcacagcg attgcatact catggcagtc ggacatcgcg acgacctcgg
     1321 aaacctccat cgatgttcgg ccaggcccgg cgtatagtcc cgcggatcgt gcgccgggcc
     1381 gctttttttg gcttcctcga ataggagggc cgcatgtggg gatttctgat cggcctctat
     1441 attgcattgg tcatctaccc ttaccttcaa gaatggcacg aacgtagggc aaacaaggct
     1501 cgcctggcga aaatgcgaaa gcatcacgcg gccggccatc gatgggatgt gaggaagggt
     1561 caatggatcg acaagtagcc gagcgctcgc gcactctggg gtaggtgaaa agggcggtgc
     1621 ccggctactc ggcccataaa cgacgcgggc aagcataagt agccaccgat tcgtggcgaa
     1681 aacccgcgcc ctccagtcca ccacccttat gcaccgagct ggaactggcg atctatcgtc
     1741 acgcggcaac caagttgctc acgcaatttc cacaggttgt tttgggaatc ctctggatgt
     1801 ttggccggcg caccatcaga ctgctgccgt tgcaggggag acatcaatgt ccagaaactt
     1861 tggcgtgccg cggcaaaccc gcgacgccta tcgtgtaacc acgtggtcgc ttgtggcggt
     1921 gaccttgatc atgatcggcc tgatcaacgt ggccgcgcat ttgagtcact ggtaacccgg
     1981 aggttcggca gcaattgcga gctgtccggt gcgtaagtta gcgggctctt tccgagtttc
     2041 cttgcgcttg ctcaagctgc tgaaacagca attggttctc ccacgtgcat ggaaaggata
     2101 ctttccttgg agccccaaaa cgcgggtaca ccgtgattgt gccagagatc caagagcgca
     2161 gttttgggga ttctttgtca gctgttttca tattgctttc caagaaatat agagggcgcg
     2221 ttcgccgcaa tttttttctg cactggcgcg ctaac
//
LOCUS       DATMGX010000150         2419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_148363, whole genome shotgun sequence.
ACCESSION   DATMGX010000150 DATMGX010000000
VERSION     DATMGX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148363"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VNR65_02455"
     CDS             complement(<1..404)
                     /locus_tag="VNR65_02455"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWJ17566.1"
                     /translation="MKRSASAVWTGDLRSGTGTISTESGVLSETPYSFKTRFEHGTGT
                     NPEELIGAAHAGCFSMALSVELQKAGLIAERIATKADVTLEQKVGGFAITAVHLTLRA
                     KVPGASQSDFEEATQAAKTGCPVSKLLRAEITL"
     gene            585..1316
                     /locus_tag="VNR65_02460"
     CDS             585..1316
                     /locus_tag="VNR65_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HWJ17567.1"
                     /translation="MPSSHLQYLPIPWPIYVLLFGALAIIATLIQIGLIRYVYTRLGI
                     SPLAALLLLLASLFGSYVNIPLAQLPGETSVVGERVDFFGFEYVVPTEVDWPGTIIAI
                     NLGGAVIPIALSLYLLFKNNIWLPAIIATAVVTAVCYAVAEPVRGAGIAIPIFIPPLV
                     ACAISCIVAWSQAAPVAYVSGSLGTLIGADLMNLPAIRDLGTPVASIGGAGTFDGIFV
                     TGIGAVVLASLIGSIGARRAHPGPA"
     gene            1469..>2419
                     /locus_tag="VNR65_02465"
     CDS             1469..>2419
                     /locus_tag="VNR65_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWJ17568.1"
                     /translation="MKTSKLEGLRERRRRIMAGGGDDKIKARHEKGLMTARDRLAALY
                     QPDTFQESGMHIAHSGRHFGTDGKDIPADAIVTGTGYVNGQLVAAVAQDFTVSGGTVG
                     KMHATKIVETMKFAQKFGIPFVAFKDSGGARIHEGVDSLSGYGDVFYQNVLLSGVVPQ
                     IAVVCGPCAGGAAYSPALMDFVIMTRNAHMFITGPEVIKAVTGRTTSMEEVGGAAMHA
                     SVSGNVHFIAEDDRHAIQIVHRLLSYLPANNTEDPPHHIGRDIVMDRDEAMNDLLPSD
                     PAEAMDVRRIIAHLVDGGELLEVHAEFAKNIIVGFARIEGL"
BASE COUNT          446 a          725 c          722 g          526 t
ORIGIN      
        1 agcgtgatct ccgcccttag aagcttcgaa acggggcacc ctgtcttggc ggcttgggtc
       61 gcttcttcaa aatcggattg ggaggcgcca ggcaccttcg cgcgaagtgt caaatgaaca
      121 gcggtgatgg cgaacccgcc caccttctgt tcgagcgtca catccgcttt cgttgcgatc
      181 ctctcggcga tcagccccgc cttttgaagc tcgaccgaca atgccatcga aaagcatccc
      241 gcatgcgcag cgccgatcag ttcctcaggg tttgtgccgg tcccgtgctc aaaccgcgtc
      301 ttgaacgagt atggcgtctc ggacagaacg ccgctctcgg tgcttatcgt tcccgtgccg
      361 gatctaagat cgccggtcca aacggcgcta gcagagcgct tcatgcggta tctcctctct
      421 agggttcctc agtctgatga tttataacga agcagccact tttttctaga tgcgcagact
      481 ccaacctgcc actgccaccg cttcttaccc atatctcgta cgccgcgctc agtgtacaaa
      541 gtgctggccg cgtggaaccg gaaccttttt tcgaggaagc cgctatgccg tcgagccacc
      601 ttcaatattt gccgatacct tggccgatct atgttctttt gttcggtgcg ctcgcaatca
      661 ttgcgacgct cattcagatc gggctgatcc gctatgtgta tactcgcttg ggtatttcac
      721 cgctggccgc gctgcttctg ctgctggcga gcctctttgg ctcttacgtc aacattccgc
      781 ttgcccagct tccgggtgag accagcgtgg tcggggagcg agtcgacttc ttcggatttg
      841 aatatgtcgt gccgacggag gtcgactggc cgggcacgat tatcgcgatc aatctcggcg
      901 gcgccgtcat ccccattgcg ctttcccttt atctcctgtt taagaacaac atctggctac
      961 cggcaatcat cgcaaccgcg gttgtgaccg cggtttgcta tgcggtggcc gagccggtgc
     1021 ggggcgcggg cattgccatc ccaatcttca ttccgccgct ggtggcctgc gcaatctcct
     1081 gcatcgtggc gtggagccag gcggcgccgg ttgcctatgt gagcggcagc cttggaacgc
     1141 tgatcggcgc ggatcttatg aacttgccgg ccatccgcga ccttggcacg cctgtggcct
     1201 cgatcggggg cgcgggaacc ttcgacggca tattcgtgac gggaattggc gccgtcgtgc
     1261 tggcgagcct cataggctca attggcgccc gcagagcgca tccgggcccc gcttaacggg
     1321 tctgttttgg atttggcctt agttggttag ggcggaaacg acatttgccg tcaacgcaag
     1381 gggcttgttg cctcgttgac aaaaaaatag aagatatttc acaattggcc aaacaatctg
     1441 cgcacgacgc ggaattgagt gaggggagat gaagacgtcc aaactcgagg ggctccgtga
     1501 gcgccgccgt aggattatgg ccgggggcgg ggacgacaag ataaaggcgc gccatgagaa
     1561 aggcctcatg acggcccgcg atcggctcgc cgctctctac cagcccgaca cctttcagga
     1621 gtccggcatg catatcgccc atagcggccg gcatttcggc accgatggca aggatattcc
     1681 ggcggatgcg atcgtcacgg gcacgggcta cgtgaacggc caactcgtcg ccgcagtcgc
     1741 gcaggatttc actgtctccg gcggcacggt cggcaagatg cacgcgacaa agatcgtcga
     1801 gacgatgaag ttcgcgcaga aattcggcat ccccttcgtc gccttcaagg attccggggg
     1861 cgcccgcatt catgagggcg tcgactcgct ctcaggttat ggcgatgtct tctaccagaa
     1921 tgtgctcctg tccggcgtgg tgccgcagat tgccgttgtg tgcggaccgt gcgccggagg
     1981 ggcggcctat tccccggcgc tgatggattt cgtgatcatg acgcgcaacg cgcacatgtt
     2041 catcacgggg ccggaggtca tcaaggcggt tacggggcgg acaacctcga tggaggaagt
     2101 gggcggcgcg gccatgcacg cctccgtcag cggaaacgtt catttcatag ccgaggacga
     2161 caggcacgcg atccagatcg tgcatcggct cttgtcctat ctgccggcca acaataccga
     2221 ggatccgccc catcatatcg gccgcgacat cgtgatggac cgcgacgagg cgatgaacga
     2281 tctccttccg agcgatcccg ccgaagcgat ggacgtacgc cggatcattg cgcatctcgt
     2341 cgacggcggc gaattgctcg aggttcacgc tgagttcgcg aagaatatca tcgtcggctt
     2401 cgccaggatc gaaggtctc
//
LOCUS       DATMGX010000151         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_273308, whole genome shotgun sequence.
ACCESSION   DATMGX010000151 DATMGX010000000
VERSION     DATMGX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273308"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VNR65_02470"
     CDS             <1..335
                     /locus_tag="VNR65_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWJ17569.1"
                     /translation="GSQAMAIGHEGIIAAGLGLARLKLWSALSFLFRGRRALWYSITD
                     RRLSHPEEFRADMAALFELLRNGAIHPVVTGREPLAAAKNVHARIDAGGLGGKIVLLP
                     WPASQLVA"
     gene            534..689
                     /locus_tag="VNR65_02475"
     CDS             534..689
                     /locus_tag="VNR65_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17570.1"
                     /translation="MKPMLANPKSIIAQVDGSGTLPEMEHESPTQPIEAPEMNTDSIL
                     GPLTSAT"
     gene            complement(870..2060)
                     /locus_tag="VNR65_02480"
     CDS             complement(870..2060)
                     /locus_tag="VNR65_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17571.1"
                     /translation="MAVQSGSDEAAMMNLRRISAVPTPLLRSVILATGVVGLLAALGP
                     AVIQNTGAAPSRDISAPTRFTELELGGAPQPSSMLNDDLGQTDVAKAELSLAGAVADA
                     SIALNKPEPVVEAASTNSPDVPPAEPAPVRVATANPSDLEPGDAGKAVSSIEIVDECL
                     VVDICVDRYLWALYQRTPKEDTIKELERRNVSVKRKGKMVTVARTFTKLVDEDFGWKD
                     PKAAERAGMSMMDYVIGGMDRSFKLRLFHTLHAAEAAGLSPGITSAFRDDYRQSIASG
                     LKAASNRSYHGGSSRGGYGHGLAADIVSVQGGTRDQRWASSEKLWKWVDAHGKEFGIG
                     RPYLGRDPPHVAPVDGQEYAAHHRGSKQARSDVKKVKRVAIRDDPSLAKRPRAAATSK
                     MRAS"
BASE COUNT          458 a          772 c          728 g          530 t
ORIGIN      
        1 atggctccca ggccatggca atcggacacg aaggcattat cgcggccggg ctgggattgg
       61 cgcggttgaa gctatggagc gcattgagct tcctattccg cggccgtcgt gcgctttggt
      121 acagcatcac tgaccgccgc ctgtcgcacc ctgaagagtt tagggctgac atggctgctc
      181 tgttcgagtt gctccgcaac ggtgccattc atccggtggt taccggtcgc gaaccactgg
      241 ctgccgcgaa gaatgtccac gcgcgcatcg acgctggtgg gctgggcggc aagattgtgc
      301 tgctaccatg gccggcctcg cagcttgtcg cgtaacccat tcatcgcttc gcgatgctct
      361 tgagtgtgct accaatctga gcgtaagttt cgacttccgg ttttggcacg atttcggaag
      421 tcgcccaact gacgcggcca aaaaaagccg cccggtggcg gctcttcaat ttcaatctga
      481 tgttggtgcg tcaggtagcg ataaatgctg gctttgactt ccggcgatac gccatgaagc
      541 cgatgctcgc gaaccccaaa agcatcatcg cccaggttga cggctcgggg acgctgccgg
      601 agatggagca tgaatcgccc acgcaaccaa ttgaggcccc cgagatgaat acggactcga
      661 tattgggacc gttaacttcc gcgacctgaa aaagagggga cagaccgccc gtgccgggtg
      721 tattgaaaaa attatgcgtc gggtcgccgt tctccgctag taggccaacc gctgttcccg
      781 ccgccgtttc gacgaagcca cttgaaggcg ggttaccacc gccatttcgg atttcttatt
      841 ttgcgagcct catatcggaa ccggggcgct cagctcgctc tcatcttcga ggttgctgca
      901 gctctcgggc gcttcgcgag actcgggtca tcccgtatgg ccacgcgctt caccttcttc
      961 acgtccgatc tcgcctgttt ggagccgcgg tggtgcgccg cgtattcctg gccgtcgacc
     1021 ggtgcgacat gcggcgggtc cctgccgaga tatgggcgcc cgattccgaa ctccttccca
     1081 tgcgcatcaa cccacttcca gagcttttcg gaggaagccc atcgctggtc gcgagttccg
     1141 ccctggacac tcacgatatc ggccgcgagc ccatgcccat agccaccgcg ggagctcccg
     1201 ccatgatacg acctgttgct cgccgccttg aggccgcttg cgatcgactg gcggtagtca
     1261 tcacggaatg cgctggtgat gccgggcgaa aggcccgccg cctccgccgc gtgaagcgta
     1321 tgaaacagtc ttagcttgaa gctccgatcc atgcctccga tcacgtagtc catcatcgac
     1381 atgccggctc gttccgccgc cttcggatcc ttccacccaa aatcttcatc gacgagcttg
     1441 gtgaaggttc tggcgacagt caccatcttg cccttccgct tgacggagac gttcctccgt
     1501 tcaagctcct tgatggtgtc ctccttgggc gtccgttgat aaagcgccca cagatatcga
     1561 tcgacgcaga tgtccacgac caggcactcg tcgacgattt cgatggagct cacggccttg
     1621 ccggcgtcgc caggttcgag atccgacgga ttcgccgttg cgactcgcac aggcgctggt
     1681 tcggctggcg gcacatccgg cgaattggtc gaggccgctt cgacaaccgg ctccggcttg
     1741 ttcagagcga ttgatgcgtc ggcgactgct cccgcaagcg atagctcggc ctttgccaca
     1801 tcggtttgac cgagatcgtc attgagcatc gagcttggct gcggtgcccc tccgagttcc
     1861 agttcggtga agcgagtggg agcgctgatg tccctgcttg gcgccgcgcc ggtgttctgg
     1921 attactgcag gcccgagggc agcaagcaag ccgacaaccc cggtcgcgag aatgaccgat
     1981 cgcaacaatg gcgtcggcac agcagagatt cgccgcaggt tcatcatcgc agcctcatcg
     2041 gaaccggatt gcacagccaa gctcggacgc ccctgattgt tcggacaagg caacggccgc
     2101 gcaatggcgc aaacggaggg gtcgtgtcct tttcccgcaa gtgttgcctg tgtgcaacgg
     2161 caggcggcag gcctgcgaac gcccgcgcga tgggctacga tcaccataag ggtgggcacg
     2221 caggcccgaa aagaacgaga cttcaacctg gcgtattcgt gtgcgctatt gatcgcatac
     2281 cggactcaac aactcggaca taatgccttg tccttttttg tgcccatttc tgctgcgagc
     2341 catccttcct cctccatggg ccgtcgcata tgagagattt gtcaccgcaa cgtgccgaat
     2401 gccccagaag cgaatcggct acgtggatga tggcggcggt tttttttggt ccgaagaccg
     2461 catctgacac tcgatcgacg caagcgcg
//
LOCUS       DATMGX010000152         2674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_396829, whole genome shotgun sequence.
ACCESSION   DATMGX010000152 DATMGX010000000
VERSION     DATMGX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396829"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1564)
                     /locus_tag="VNR65_02485"
     CDS             complement(284..1564)
                     /locus_tag="VNR65_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HWJ17572.1"
                     /translation="MKFSKQALFAKLVVALLSVFLVQAALAGTPSTEGFRAWIASFRA
                     KALEKNIRASVYDSVTRGLVPDFSLPDLDIPSKKQAEQPEFVRTPEQYLGDKSLASLA
                     EQGRRLFAQYKDTLIAVKTAYHIDPYILLAVWGRETAFGTFANDSHYNALEVLATEAY
                     AGRRKELFERQFIEGLRMVQEGVIAPQDMKSSWAGAMGLVQFMPEDYFKYAVDQDGDG
                     KKDIWHSVPDALASLAKNLQGINWNSQHPWGLEVRAPAKVDCSLGYLDIRKPVSEWAA
                     MGIAPVKGGEFPPEVMSWEASLLQPAGIFGPAFLTFDNFQVIREYNKSDLYALFVGNL
                     ADRIRGGGSFSQPWQRIVQVPTSDVEDMQKRLTGLGLYGDTIDGKAGGRTRAAVGAYQ
                     KRFGLPQTCWPTAEIVAHLKAHGGAQTTNERAQN"
     gene            1806..>2674
                     /locus_tag="VNR65_02490"
     CDS             1806..>2674
                     /locus_tag="VNR65_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17573.1"
                     /translation="MSGAYINQIATAVPPHQVHNAFVQFQRHMLDDYRKQTIFDRLVE
                     KGQIEKRWSCVVPAEDCSGATINGASFYVPGDFPSTGERMRQYEIEAPVLAECAINRL
                     NLGDRKSEITHLIVTSCTGFSAPGIDLEMIHRIGLNPSIERTIIGFMGCYAAVNALKV
                     ARHIVRSEPKAKVLVLSIELCTLHFQETYELEEMMPFLLFADGCAAALISAEPRGLSI
                     ERFYATVLPEAASQMAWHIRDLGFDMVLSSRIPTSVGEAIRRASDAILAGLKPKDIEL
                     WAVHPGGRAILDAV"
BASE COUNT          574 a          811 c          751 g          538 t
ORIGIN      
        1 cgcctgccaa agcaacggcg aaaaacccga agccggatcg ctggcctaaa agtttcgcgc
       61 agaaattgcc gtcgcccgcc aatacaagca cgcctgccag gcaaagggcg ggggcatttc
      121 ggaagcaatg acgagaagaa agggctcggc gccgggacat ttgttgcgtc catgacaagt
      181 cagaataacg gttattcgat aacgcacacg gctcgtccct attccgcctc catgttgaaa
      241 ttaaggaccg gaccgaaact aacaggaaaa agggcagcgt cttttaattc tgcgcgcgct
      301 cgttcgtggt ctgggcaccg ccatgtgcct tgagatgcgc cacaatttca gcggtaggcc
      361 agcacgtttg cggcagccca aagcgcttct ggtaagcgcc gaccgcagct cgggtgcgcc
      421 cgccagcctt gccgtcgatc gtatcgccat aaaggccgag gccggtgagc ctcttttgca
      481 tatcctcgac gtccgaggtc ggcacctgga caatccgctg ccaaggctgc gagaagctgc
      541 cgccaccgcg gatgcggtct gccagattgc ccacgaaaag cgcgtagaga tcggacttgt
      601 tgtattcgcg tatcacctgg aaattatcga aggttaggaa agcaggcccg aatattcccg
      661 cgggctgcaa gagagaggct tcccagctca tgacttcggg tggaaattct ccgcccttca
      721 ccggggcaat ccccatcgcg gcccattcgc tgaccggctt gcgaatgtcg agatagccga
      781 gagagcaatc gaccttcgcc ggcgcgcgaa cttcgaggcc ccaaggatgc tgactattcc
      841 agttgatgcc ttgcagattt ttcgcaagcg acgcgagcgc atccggcacg gaatgccaga
      901 tatccttctt accatcgcca tcctggtcga cggcgtactt gaagtaatct tccggcatga
      961 actgcacgag ccccatcgcg cccgcccagg agcttttcat gtcctgcgga gcgatcacac
     1021 cctcctggac catgcgcagc ccctcaatga attggcgttc gaacaactcc ttgcgccgtc
     1081 cggcataggc ctcggtcgcc agcacctcaa gcgcattata gtgcgaatcg tttgcgaagg
     1141 ttccgaatgc ggtctcccgg ccccaaacgg caaggaggat gtaggggtcg atgtgatagg
     1201 ccgtcttgac ggcgatcagg gtatctttgt actgggcaaa gagcctgcgg ccctgttcgg
     1261 cgaggcttgc gagcgacttg tcacccaaat attgctcggg cgtgcggacg aactccggct
     1321 gctccgcctg cttcttggaa ggaatgtcga ggtccggaag agagaaatcg ggaacaagcc
     1381 cgcgcgtaac ggaatcgtag acgctcgctc ggatattttt ttcaagcgct tttgcacgga
     1441 acgaagcgat ccacgctcga aacccctccg tggagggcgt gccagccagc gccgcctgaa
     1501 caagaaacac agacaaaagc gccaccacga gtttcgcgaa tagggcctgc ttactgaact
     1561 tcatcgtctc ggaattcccc ttgggatacg aaagcagcga cactatcagc caatgtgtcg
     1621 atctctgcca catgcggcct tgccaaagct tactcggcca tccgcccgga aaccccagcc
     1681 atgactggag cggcgcttga agccccccca cattgcgcct atcctcagag ttgggcggga
     1741 ttgcgaacaa cgccttcagc aaattggatt tccctgcaat ggttaacgct tgaggaaggt
     1801 gcgccttgtc cggagcatac atcaatcaga ttgcgactgc tgtccctccc catcaagtcc
     1861 ataacgcttt tgtccaattc caaaggcaca tgctcgatga ctaccgcaag caaacgatct
     1921 tcgaccgtct ggtcgagaaa ggccaaatcg aaaagcggtg gtcctgtgtc gtacccgccg
     1981 aagactgttc aggcgccacg ataaatggcg cgagcttcta cgtgccgggt gattttccct
     2041 cgaccggcga acgtatgcgg caatatgaaa tcgaggctcc cgtccttgcc gagtgcgcta
     2101 tcaaccggct aaacctcgga gatcgaaaga gcgaaatcac gcatctgatt gtcacctcct
     2161 gcaccggctt ctcagcgccg ggcatcgatc tggagatgat ccaccgcatc ggcctcaatc
     2221 cgtccatcga gcgcacgatc atcggcttca tgggctgtta cgcggccgtg aacgctctca
     2281 aggtggcgcg tcatatcgtc cgctcggagc caaaggcgaa ggtgctcgtg ttgagcatcg
     2341 agctttgcac cctgcatttc caggagacct atgagctcga ggagatgatg ccctttctgc
     2401 tctttgccga tggttgcgcg gcggcactca tcagcgcgga gccgcggggg ctttccatcg
     2461 aacggttcta tgccacggtc cttcccgaag ccgccagcca gatggcttgg cacatccggg
     2521 acctcggttt cgacatggtt ctttcaagcc gcattccaac ctccgtcggt gaggcgatcc
     2581 ggcgcgcctc cgacgcaatt ctcgcggggc tgaagccgaa ggatatcgaa ctttgggccg
     2641 tgcatccggg agggcgagcc attctcgatg cggt
//
LOCUS       DATMGX010000153         2669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_216149, whole genome shotgun sequence.
ACCESSION   DATMGX010000153 DATMGX010000000
VERSION     DATMGX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216149"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /gene="pstC"
                     /locus_tag="VNR65_02495"
     CDS             complement(<1..1342)
                     /gene="pstC"
                     /locus_tag="VNR65_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWJ17574.1"
                     /translation="MSLTSLLLCLLGLAVAAFFVGFARARILAHGQERSLHSRPGYHG
                     GYLALWTSLPAVLVLLIWAAVEPRLIDGQVQTTLPAQVLQGSEAQIGLTMSVVRSVAS
                     GLKTFSREELQAAKADPQEAQRLLSQRGIVVASAPDAAIIQSAQLLDNLKRKSRLAKT
                     FVAALLAIAGFAFAFFGISLNLRARNRVERVIVAGLMAASSVAILTTVGIVASVLFES
                     LDFFKRVSIVDFLTGTVWDPRFADAGATGNTEGQFGLVPLMWGTLYISFVALLVAVPI
                     GLLAAIYMSEYAHPKVRATVKPLLEMLAGIPTIVYGFFAVITVGPFLNFVGSKAGLVI
                     QPTSVLTAGIVMGIMIIPFVSSLSDDIISAVPRSLREGSYGLGATQSETIKRVVLPAA
                     LPGIVGAILLAASRAIGETMIVVLAAGIAANLTLNPFESVTTITVKIVSQLTGDL"
     gene            complement(1420..2505)
                     /locus_tag="VNR65_02500"
     CDS             complement(1420..2505)
                     /locus_tag="VNR65_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWJ17575.1"
                     /translation="MNYRLTAYIAATAAAGLLSAGSASARDQVKVAGSSTVFPYAKIV
                     AENFAKANSTQKAPVVESGGSGAGIKQFCDGVGEDKIDIANSSRRIKKTEQDNCAKNG
                     VAEIVEVKFGYDGIVFASDVKGPSFAFEPKDWFLAIGAEVPVNGALAANPNKTWKEVN
                     PNFPGWDIVSYIPGEKHGTREVFETKVLVAGCKASGADKLIEEKLKASGTPAEKLSEA
                     TEKACAKVRKDDGGKHAVDIDGDYTETLARLQSNKQALGVFGLSFYENNTDKLKVAPV
                     NGVTPSVETIADAKYPVSRPLFFYVKKAHEGIIPGLKEYAEFFVNDKMIGEDGPLAQY
                     GLVPLPEKEAKETRETVKSLKTMSPLS"
BASE COUNT          591 a          756 c          798 g          524 t
ORIGIN      
        1 cgaggtcgcc ggtgagttgg ctcacgattt tgaccgtgat ggtcgttacg gattcgaagg
       61 ggttcagcgt gaggttggcg gcaatgcctg cagccagcac cacgatcatg gtttcgccga
      121 tggcgcggga cgctgcgagc aggatcgcgc cgacaatgcc ggggagcgcg gcgggcagca
      181 ccacacgctt gatggtttcg gactgggttg cgccgaggcc gtaggagccc tcgcgaaggg
      241 accgcggaac ggcggagatg atgtcgtccg aaagcgaaga cacgaaggga atgatcatga
      301 tccccatgac gatgcctgcc gtcaaaacgc ttgtcggctg tatgacaagg cccgctttcg
      361 atccaacaaa gttcaggaat ggccccacgg tgatgaccgc gaagaacccg tagacgattg
      421 tcgggatacc cgccagcatc tccagcagcg gcttgacggt tgccctgacc ttaggatgcg
      481 catattccga catgtagatc gcggccagca atccgattgg cacagcaacg agcagcgcta
      541 cgaaggagat ataaagagtg ccccacatga gcggcacgag cccgaactga ccttccgtat
      601 ttccggtggc gcctgcgtct gcaaaacgcg gatcccatac ggtgcctgtc agaaaatcga
      661 caatcgacac gcgcttgaag aaatccaggc tctcgaagag caccgaagcc acaattccga
      721 cggtcgtaag gatcgcgacc gaagacgccg ccatgaggcc agccacgatg acccgctcga
      781 cgcggttgcg ggcgcgaagg ttcagcgaaa tgccaaaaaa agcgaaggca aagccggcaa
      841 tggcgagaag ggccgcaacg aaagtcttag caagacggct tttgcgcttc aggttgtcaa
      901 ggagttgagc gctttggatg attgccgcat cgggtgcgct cgcgacaacg attccccttt
      961 gcgaaaggag gcgctgcgcc tcctggggat cggcctttgc agcctggagt tcctctctgc
     1021 tgaatgtctt gaggccggat gcaacgcttc ttacgacgct catcgtgagg ccgatctgcg
     1081 cttccgagcc ttgcagcacc tgcgcgggta aggtcgtctg gacttgccca tcgatcaagc
     1141 gcggctcaac agcggcccag atcagcaaaa cgaggacagc tggaagcgag gtccatagag
     1201 cgagatagcc gccatgatag ccggggcggg aatgaagcga cctttcctgg ccatgcgcca
     1261 ggatcctggc ccgcgcaaat ccaacaaaaa atgcagcgac cgccaaacca agtagacaaa
     1321 gtaataatga ggtgagagac acggccgact cccgaagcgc gccaagcgag gtgatgcaac
     1381 atagcgtggc ggctgccccg ccgccacgcg cattgcgtct tagctgagcg ggctcatcgt
     1441 cttcagggac ttgacggtct cgcgcgtctc cttcgcctct ttctccggaa gcggaacaag
     1501 cccgtactgg gcgagaggtc cgtcctcacc gatcattttg tcgttgacga agaactcggc
     1561 atactctttc agaccgggga tgatgccctc atgagccttc ttcacataga agaagagggg
     1621 ccgggagact ggatatttcg catccgcgat cgtctcgacg gaaggcgtaa cgccattcac
     1681 gggtgcaacc ttcagcttgt cggtattatt ctcataaaag gagagaccga atacgccaag
     1741 agcctgcttg ttggactgaa gacgagccag cgtctcggtg taatcgccgt cgatatcgac
     1801 agcgtgcttg ccgccgtcat ccttgcggac cttcgcgcaa gctttctcgg tggcctcgct
     1861 cagcttctca gcgggcgtgc cgcttgcctt cagcttctct tcaatgagct tgtccgcgcc
     1921 agaggctttg cagcctgcga ccaacacctt cgtttcgaaa acttcgcgcg ttccgtgctt
     1981 ctcgccggga atgtaggaaa cgatgtccca accggggaaa ttcgggttga cctctttcca
     2041 agtcttgttg ggattggctg caagggcacc gtttaccggc acctcggcgc cgatggcgag
     2101 aaaccaatcc ttcggttcga acgcaaacga ggggcccttc acgtcggaag cgaaaacgat
     2161 gccgtcataa ccgaacttga cttcaacgat ttcggcgacg ccattcttcg cgcagttgtc
     2221 ctgctcggtc ttcttgatgc ggcgcgaaga gttggcgatg tcgatcttat cctcgccgac
     2281 gccatcgcag aactgcttga tgcccgcgcc tgaaccgccc gactcgacaa cgggggcctt
     2341 ctgggttgag ttcgccttcg cgaaattctc ggcgacgatt tttgcgtagg ggaagacggt
     2401 cgaggaacca gcgactttga cctgatcacg agcggatgcg gagccagcgg aaagaaggcc
     2461 ggcagccgcc gtagccgcaa tatatgcggt gagacgataa ttcactgtgc tctcccttgg
     2521 gaatatgaaa ccaaccggca acctgttggc ggtcggtgcg cttggatcgt aggatagcgc
     2581 tacaactgtt ttatgaaagc tggatgaacc ttttgtgaca gagcaaggta ttattccaga
     2641 aaggaaaggt acgcctgagg cacaattcg
//
LOCUS       DATMGX010000154         2356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_216113, whole genome shotgun sequence.
ACCESSION   DATMGX010000154 DATMGX010000000
VERSION     DATMGX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216113"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VNR65_02505"
     CDS             <1..215
                     /locus_tag="VNR65_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17576.1"
                     /translation="NCGPMRERQPVAGIQSSTPYFDGLTAHSNHPVRRRTVSKQLHRE
                     NRRQLDRQSMKESGTYGTHGDLPQVI"
     gene            complement(212..541)
                     /locus_tag="VNR65_02510"
     CDS             complement(212..541)
                     /locus_tag="VNR65_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17577.1"
                     /translation="MRGLIFYLLRPEPEPVGVNVDPLGEALGPRVFPDGLWVLFGEVT
                     DAPAVPVVLPLDVEPVELPVAAEPAEVPPAPDADAPPACASANVLESAKAVPNAIVAS
                     FMVVSFS"
     gene            complement(1000..1290)
                     /locus_tag="VNR65_02515"
     CDS             complement(1000..1290)
                     /locus_tag="VNR65_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17578.1"
                     /translation="MTTQRRKIGNRLTPSENRPVVDKYPARQFPIQREIRKLEDELDA
                     FNERILELEGEGHRGHAMQVLEATAIDFARQIDELRCVLVESARVTFLGEKI"
     gene            complement(1564..1980)
                     /locus_tag="VNR65_02520"
     CDS             complement(1564..1980)
                     /locus_tag="VNR65_02520"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HWJ17579.1"
                     /translation="MDLFIVPTIGFDLLYAFVIVRLDRRDLVWINVTANPTAEWVAGQ
                     ITEAFPWDEAPPHLIRDRDRIYGSVVTRRLRAMGIRDKPTAPASPWQNGFAERLIGSI
                     RRECVDHIIILGEMHLRRVLKSYADYYNLRQNAPIL"
BASE COUNT          525 a          671 c          625 g          535 t
ORIGIN      
        1 agaattgtgg accaatgcgt gagcgacagc cggtagccgg tattcaatct tcgacgcctt
       61 attttgacgg cctcacagcg cactcaaatc atccggtacg acgccgaact gtctcaaaac
      121 agttgcatcg cgaaaatcgc cggcagctgg atcgccagtc gatgaaggag agcggaacat
      181 atggcactca tggcgactta ccccaggtca tttaactaaa ggaaacgacc atgaaacttg
      241 caacaatcgc attgggtacg gcctttgcgc tttcgagcac attcgcactt gcacaagccg
      301 gcggcgcatc ggcatcgggc gccggcggaa cttccgcagg ctccgcggcc accggcagct
      361 cgactggctc gacgtcgagc ggcagcacga ccggaacggc gggcgcgtcg gtaacttctc
      421 cgaataacac ccagagtccg tcagggaaca ccctcggacc caaagcctcg ccgagcgggt
      481 caacattgac tcccacaggc tcaggttcgg gtctcagcaa gtaaaagatt aagccccgca
      541 tcttcgcgga ctctttacgc acagccttct gaagcacaaa cccctgccgg tcttggccag
      601 caggggtttt tgcatgtgga tgacgaacgg cgagctatac gctcggtggc tcttcaaaga
      661 ccatgcttaa acatagatgc ccaaggcacg aagcgctcaa gattaattag taggcatcgc
      721 agctcgaccg gtatgcatcc agcccgcatt agcggcgcgt attcaaggag accccaagtt
      781 ttgctgccat cgcgcttatt gcaccgggtg agcgcttcaa tgcctttgaa attctcttta
      841 ctcccaccct tttcttcgcc agcgacttca atttgcgcac ttcggacgct gtccatgtgc
      901 gtctaactac tttcttctta gccattccgg tcctcatttt ttcgaaccgg gcctgtatca
      961 gcgtacactc agctcgtaat gcggcgacgt gatttgcgtt taaatctttt ccccgaggaa
     1021 agttacccga gccgactcta ccaatacgca acgcagttca tcgatttgtc gcgcaaaatc
     1081 tatcgcagtt gcctccaaca cctgcatggc atgaccacga tgtccttcac cctctaactc
     1141 taagatgcgc tcgttaaatg cgtcgagttc gtcttccaat ttgcgaattt cgcgctgaat
     1201 cggaaactgt cgagcaggat atttatcgac gactggtcga ttctctgaag gcgtcagtct
     1261 atttcctatt tttcgccgct gtgtggtcac aacccctccg gctggatgga tttacgtaat
     1321 cgtctctcgg gttctggaag ctgtgtcgaa aatcttaatc tgtaacattg ggaaccggtt
     1381 ctttaactca acagccccct tttctgcgcc ttccttggtt tggaattcgg ctggggaaaa
     1441 gccgacggcc tgtgtgtacc gaaaacttaa gctcgggcgt agtggtggtg aagtccgcca
     1501 aggatggcgt gtgatttgat gcttccgagt cgctgaattt ggcgagagac tggtgcatcc
     1561 ttgttaaagg atcggtgcgt tctgacgcaa attgtaatag tcggcgtagg atttcagaac
     1621 ccgacgcaaa tgcatctcgc ccaagataat gatgtgatcc acacactcac gccgaatcga
     1681 tccgatcagc cgttcggcaa aaccattctg ccaaggcgag gctggtgcgg taggcttgtc
     1741 ccggataccc atggcgcgca atcggcgtgt gacgacgcta ccatagatcc ggtcgcgatc
     1801 gcggatgagg tggggcggag cctcatccca aggaaatgct tccgttattt gacctgcaac
     1861 ccattctgcc gtcggatttg ctgtgacgtt gatccagacg agatctctgc ggtccagccg
     1921 gacgatgacg aaggcataga gcaggtcgaa gccaatggtc gggacgatga acaggtccat
     1981 ggcggcaatg tccgcgcgtg gttacgcaga aaggtgcgcc atccctgact taaaggtccg
     2041 cgtcgcttga ccatgtattt ggcgacgctc gactgtgcga cttcaagccc gagcttgagc
     2101 agttcgccgt gaatgcgtgg cgcaccccaa agcggattct ccatgctcat ccgccggatc
     2161 aacgcgcgca gctccgtgtc gatccgcggt cgccctccca atgggagcga cttccaacgc
     2221 caatagcagc gaaagccggc cctgtgccaa cgcacgagcg tctcgggctg gatgattgta
     2281 agaaccttca ggattgatgg aaaccagcga tacagctgga taaagaacca gcgatcattg
     2341 ttcgtgagcc ggacgc
//
LOCUS       DATMGX010000155         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_125633, whole genome shotgun sequence.
ACCESSION   DATMGX010000155 DATMGX010000000
VERSION     DATMGX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125633"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VNR65_02525"
     CDS             <1..214
                     /locus_tag="VNR65_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17580.1"
                     /translation="RNLSRGPLSAASDNQIEPKVSAFRCEWFGQQESLGIGASVIVTT
                     KYAPIEGMHTLRNCGKIKLPAGGAVL"
     gene            211..756
                     /locus_tag="VNR65_02530"
     CDS             211..756
                     /locus_tag="VNR65_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17581.1"
                     /translation="MRNLDLIDWTMQEARQLNPGHHIAIEPNGSASLTKLLEALESER
                     IKQSADDTIVQFTAAPIEPHGDSSTPRPSPKLTVDPKPNTAPDFFAPTQAVPSPAITA
                     AAGPKSALLKAIVEPDIYTAPIDRDRAIALRWLLRDIKSNRLKWSPINQHDLQDLIDM
                     RLVEIRNDAPVLTNAGVNAII"
     gene            complement(881..2236)
                     /locus_tag="VNR65_02535"
     CDS             complement(881..2236)
                     /locus_tag="VNR65_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWJ17582.1"
                     /translation="MRPKERRESGQKDLFKARLDQILDLDHALVKLAGAIDWRFLEDR
                     FGAVYDDDPGRPPLPTRLMAGLAILKHMHDLSDEVLCERWVENPYYQLLCGEEFFQHR
                     LVFDRSSLTRWRQRMGEEKLAALVQESLASAIRMGAARPADFTQVIVDTTVQEKAISF
                     PTDARLMHRARERLVRLARASGIKLRQSYARVGKFALIKHQRYAHAKQFKRANKALRR
                     IRTLLGRVIRDIRRKIVDNPSLGDAFALPLSLAHQVRDQRQRQRGKKIYSLHAPEVEC
                     IGKGKAHKPYEFGVKVSVTTPINRCKGGQFVLHAKALPGNPYDGHTLAVVIPEIEASV
                     GASLAKIVADAGYRGHNASKEKAFKVYVAGQKRGLSSAIKRAFRRRSAVEPVIGHLKN
                     EHRMGRNHLAGSRGDAANAVLAAAGYNFRLLFLWISILCAWFSATIYRNLQPSEILQP
                     G"
BASE COUNT          502 a          699 c          670 g          487 t
ORIGIN      
        1 ccgcaattta agccgtgggc cgttaagtgc agcatccgac aatcaaattg agccaaaagt
       61 gagtgctttc cgatgcgaat ggtttggaca gcaggaaagc ctagggatag gggcatcggt
      121 gatcgtcacg actaaatatg ccccgatcga agggatgcac accttgcgaa attgtggcaa
      181 gattaagtta ccagctgggg gtgcggtatt atgagaaatt tggatttgat cgattggacg
      241 atgcaggaag cccggcagct aaatcccggt catcacatcg caatcgaacc caacggctca
      301 gcttcgctaa ccaagctgtt ggaggctctt gaaagtgaac ggataaagca gagcgcggat
      361 gacacaatcg tccagtttac cgccgcgccc atcgagccgc atggcgacag ttcgacacca
      421 cgaccgtctc ctaagttaac tgtggacccc aaaccgaaca ccgctcccga ttttttcgcc
      481 ccgacgcagg ctgttccgtc gcccgccatc acggcggccg ccggcccaaa gtcggccctg
      541 ctaaaagcca tcgttgaacc agatatttac accgctccga ttgatcgcga tcgagcgatt
      601 gcccttcgtt ggttgctcag ggatatcaaa agtaatcggc tgaaatggtc gcccatcaat
      661 cagcatgacc ttcaagattt aatcgacatg agattagttg aaattcgaaa tgatgcgccc
      721 gtgctcacga acgctggcgt caacgctatc atctagtctt gagagtttat cgaatgaaac
      781 gtccaaggga cttggccgat cggtctcgcg atccatccaa gttgacggat gatggccttg
      841 tgcgcgtcac cgttacgttg tcgtccgtga agaacgccga tcatccgggc tgaaggattt
      901 ctgacggctg taggtttctg tagatggttg cgctgaacca agcgcacaaa atgctgatcc
      961 agaggaacag gagcctgaag ttgtagcccg cagcggcgag aaccgcgttt gcggcatcac
     1021 cgcggcttcc ggcgaggtgg ttgcgaccca tgcggtgttc gttcttgaga tggccgatta
     1081 ccggttcgac ggcggaccgg cgccggaagg cgcgcttgat ggcggatgac agaccgcgtt
     1141 tctggcccgc aacgtaaacc ttgaacgcct tctccttcga cgcgttgtgc ccgcgatagc
     1201 cggcatcggc gacgatcttc gccaggctcg cgccgacgct cgcctcgatc tcgggaatga
     1261 cgactgcgag ggtgtggccg tcatagggat tgcccggcag cgctttggcg tgcagcacga
     1321 actggccgcc cttacagcgg ttgatcggcg tcgtgacgct caccttgacc ccgaactcgt
     1381 agggcttgtg ggcttttccc ttgccgatgc attcgacctc tggcgcatgc aacgagtaga
     1441 tcttcttgcc acgctgccgt tgacgctggt cgcgaacctg atgggccagc gatagcggca
     1501 aggcgaaggc gtcaccgagg gaggggttgt cgacgatctt gcgcctgata tcgcggatga
     1561 cgcggcccag gagggttctg atccggcgca aagccttgtt cgcgcgcttg aactgtttcg
     1621 catgggcgta gcgctggtgc ttgatcaggg cgaacttgcc aacccgcgca taggactgcc
     1681 ggagcttgat gccgctcgcc cgcgccaggc gcaccagccg ctcgcgggcc cgatgcatca
     1741 gcctggcatc tgtcgggaag ctgatcgctt tctcctgcac ggtggtatcc acaatgacct
     1801 gggtgaagtc ggccggcctg gctgcaccca tgcggatggc ggatgcgagg ctttcctgaa
     1861 ccagcgcagc cagcttctct tcgcccatgc gctgccgcca ccgcgtcagt gatgaccgat
     1921 cgaacaccag ccggtgctgg aaaaactcct cgccgcagag cagctggtaa tacgggttct
     1981 ccacccagcg ctcgcacagc acttcgtctg aaagatcgtg catgtgtttc aggatggcca
     2041 gccccgccat cagtcgtgtc ggcaatggcg gccggccggg atcgtcgtca taaacagcgc
     2101 caaaccgatc ttcgagaaac cgccagtcaa tcgcacccgc cagcttcact agagcatgat
     2161 cgagatccag aatctgatcc agcctggcct tgaacagatc tttctgcccg ctctcccgac
     2221 gctctttcgg tcgcatcccg acctccgctg ctgcttcgca acgtcgatag aatcacgaca
     2281 cctacgccaa atcaatttgc aaggaaacct acgcccatcc tgtcaaaact ggcaaaaacc
     2341 aatacttaca agctgaga
//
LOCUS       DATMGX010000156         3156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_15254, whole genome shotgun sequence.
ACCESSION   DATMGX010000156 DATMGX010000000
VERSION     DATMGX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15254"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VNR65_02540"
     CDS             complement(<1..334)
                     /locus_tag="VNR65_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HWJ17583.1"
                     /translation="MGSLSIIYRKISELKPYLRNARTHKRKQIKQIKASIEEFGFTNP
                     ILIDDSDQIMAGHGRVEAAKLLGLTEVPTVQISHLSAAQKRAYILTDNRLAEKAGWDK
                     EILVCELQG"
     gene            complement(1198..2817)
                     /locus_tag="VNR65_02545"
     CDS             complement(1198..2817)
                     /locus_tag="VNR65_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWJ17584.1"
                     /translation="MKAASIRSVRCAIYTRVSTEYGLDQEFNSLDAQYDAASAYIKSQ
                     AHAGWTQIRSRYDDGGYSGGSTDRPDLQRLLDDIRSRKLDVIVVYKVDRLTRSLADFA
                     KLVELFDAHGVSFVSVTQQFNTTTSMGRLTLNVLLSFAQFEREVTSERIRDKIAASKR
                     KGLWVGGALPLGYHMKDDKIAVVEDEAERVRLIFHRYLELGGVNALVRDLRERDIRTK
                     SRLLATGATRGGVPFGRGSLFYLLRNRFYVVEVKYKDEILPGEQPAIMDRALFDAVQQ
                     KLTDQWTTRSTTRNASDHLLTGLLFDDVGHRMVPTHATKAGVRYRYYVSLPHLHGESK
                     TASVGSISRVPATDIESIIVKSLNEHLIAQKEKPASSQTHVSDSKAALEQVARIDVYE
                     DRLAVRLKSADDEEISDAGDDYLLSIPWQKPPSRRSRQILIPHGMPKSEIRPTRIERR
                     ARLVSAIARGRRWLDEMVSGSVTDVQQIASRQKCSVRQVNMTISLAFLAPDLVRAAVE
                     GRLPRGIGVERLRDAPAEWSQQFEALGLNPR"
     gene            complement(2814..>3156)
                     /locus_tag="VNR65_02550"
     CDS             complement(2814..>3156)
                     /locus_tag="VNR65_02550"
                     /inference="COORDINATES: protein motif:HMM:NF022598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HWJ17585.1"
                     /translation="AYRIQADRLGDLDHETGQVLDQTDAKDTGAKMSARLVSFDQKRI
                     ELTPGTVLVREWDRRSQRVMVMADGFAWNGQTYDSLSKVAFAITGTKWNGPRFFGLRD
                     KEARLDAKARS"
BASE COUNT          671 a          898 c          880 g          707 t
ORIGIN      
        1 agccctgcag ttcgcagact aggatttcct tgtcccagcc ggctttttct gccaatcgat
       61 tgtcagtcag gatgtaagca cgcttttgcg ctgcgctgag gtggctgatt tgaaccgttg
      121 ggacctcggt aagtcccagc agcttagccg cctcgacccg tccgtgcccg gccattatct
      181 ggtcgctgtc gtcgatcaga attgggttgg taaagccaaa ttcctcaatc gaagctttta
      241 tctgcttgat ttgttttcgc ttatgcgtgc gcgcattgcg cagatacggt ttcagctcag
      301 aaattttgcg atagatgatt gataggctgc ccatagcgag ctccttttcc ggtttgaatt
      361 ggaaaaagtc gcaatgggac tcagagtgcg agcgtgacct ttgcgacaat gcttcaaggg
      421 cctgccatgc aagcccttga gcccatcggg cgtcacgccc tcaatgtacg cgctaacata
      481 caacgattgc agctagggga ctccgaagcc gactcacgga caccatgtcc cgcaagcctg
      541 gctcgggcgg ctagccactc aaaaggccaa tccccacgaa ccatgttcaa tggggcctga
      601 cctctcgttg ctcatcacgc gaggctgtaa tgctgttata ccgagcactc gcatacagag
      661 taaagcgaaa aattgcactc ttcgcgagtt ttttaagggc tagccgaaat ggcagcatac
      721 gcctgttctg agggtaaccg cttctgtttc tagcgagatg gcgcctgata agggccaaga
      781 gagtcctgtt cagcttccta gggaatttgc tcgcaagctg ttgaaataac gcagcgaatg
      841 ctggggcatt cctgagtaac aggctcaaat tgcgcaaatt ccctgcaaaa tgccatttaa
      901 caggaccgga gaccggttcg tattagactg cccacaccac catccagtcc taccgaacca
      961 cgaatcacag ggccgactcc agataggccg tttctttggg gattttcgcc ggtattgttc
     1021 cgctctttcg gtctccggtg acacttgcgg tctctcaggc cgattttagc ctcccgtctc
     1081 tgcatccaaa aattccgttc cccgcggggc gcgggtcgac gaccaagacc gttcggcaac
     1141 ttgggaatat tggaagtctt gggaggccaa aagcgccgtt tcgagcccga tccgtaatta
     1201 tcgcggattc aatccgagtg cctcgaactg ctggctccat tctgcgggag catcgcgcaa
     1261 tcgttcgacg ccgataccac gtggcaaacg gccttcgacc gcggcgcgca cgagatctgg
     1321 tgcgaggaac gcgagcgaga tcgtcatgtt gacctgacgc acgctacact tttggcgtga
     1381 tgcgatctgc tgaacgtcgg tcacggagcc cgaaaccatt tcgtcaagcc agcgacgacc
     1441 tcgggcgatg gcgctgacga ggcgcgcgcg acgttcgatc cgcgttgggc ggatttcact
     1501 tttgggcatg ccgtgcggga taagaatctg cctggatctc ctggagggcg gcttctgcca
     1561 cgggatagag agtaaatagt cgtcgcccgc atcagatatc tcttcgtcgt ctgctgactt
     1621 caaccgaaca gctagacgat cctcatacac atcgatacgc gcgacctgct ccaaggccgc
     1681 tttgctatcg ctaacgtgag tttggctgga ggccggcttc tccttctgag cgatcagatg
     1741 ctcattgaga gacttaacga tgatgctttc gatgtcggtc gccggaacac gggagatcga
     1801 cccgactgat gcagttttgg attctccatg caggtgcgga agcgaaacgt aatagcgata
     1861 gcgaacgccg gccttggttg catgggtcgg aaccatgcga tgaccgacat cgtcgaacaa
     1921 aagtccggtc aacagatggt cgctcgcgtt ccgcgtcgtg gatcgggtcg tccactgatc
     1981 cgtgagcttt tgctggaccg catcaaatag cgcgcgatcc atgatcgccg gctgttcgcc
     2041 gggcaggatc tcatctttgt atttgacctc aaccacataa aagcgattgc gcagcaggta
     2101 gaacagcgag ccacggccga aggggacgcc gccccgggta gcacccgttg cgagcaatct
     2161 tgatttggtt cggatatctc gttcccgaag gtctcgcacc agtgcattga cgccgccgag
     2221 ttcgagatag cggtggaaga tcagccggac ccgctcagcc tcgtcctcga cgacggcaat
     2281 cttgtcgtcc ttcatgtgat agccgagggg gagggcaccg ccgacccaca ggccctttcg
     2341 tttagaggca gcaattttgt cgcggatgcg ctcggaagtg acctccctct cgaactgggc
     2401 gaaggacaga agtacgttga gcgtcagccg gcccatcgaa gtcgtggtgt tgaactgctg
     2461 ggtgacggag acgaacgaca cgccatgtgc atcgaacagt tctaccagct tggcaaagtc
     2521 ggctagcgat cgcgtcaggc gatcgacctt gtagacgaca ataacatcaa gcttccgaga
     2581 gcggatatcg tccagcaatc tctgcaggtc gggacgatcg gtggacccac cggaataacc
     2641 gccgtcatca tatcgcgacc ggatctgggt ccagccggca tgggcctgac tctttatata
     2701 agctgacgcg gcatcgtatt gggcgtccag cgaattgaac tcctggtcga gtccgtattc
     2761 cgtggagacg cgggtgtaga tggcacagcg gacggacctg atcgaagcag ccttcatgac
     2821 cgagcctttg cgtctaaccg agcttcttta tccctcaggc caaaaaagcg cggaccgttc
     2881 catttggtac cggtgatcgc gaaggcgacc ttggagaggc tgtcataggt ctggccattc
     2941 caggcaaaac cgtcagccat caccattacc cgttgcgatc gacgatccca ctctcgtacc
     3001 aacacagttc ccggtgttag ttcaatccgc ttctgatcga agctgacgag acgcgcagac
     3061 atcttcgcgc cggtgtcctt cgcgtccgtc tgatcaagca cctgcccggt ctcatgatct
     3121 aaatcgccca agcggtcggc ttgaatccga taggcg
//
LOCUS       DATMGX010000157         2055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_283901, whole genome shotgun sequence.
ACCESSION   DATMGX010000157 DATMGX010000000
VERSION     DATMGX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283901"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VNR65_02555"
     CDS             complement(<1..125)
                     /locus_tag="VNR65_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ17586.1"
                     /translation="MTKERTLHVVGAGLAGSEAAWQAAERGANVILHEMRPARLT"
     gene            236..655
                     /locus_tag="VNR65_02560"
     CDS             236..655
                     /locus_tag="VNR65_02560"
                     /inference="COORDINATES: protein motif:HMM:NF019017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HWJ17587.1"
                     /translation="MTVHILKLCVGCDSVEELADWQRKRLATGQDLVHVTRQTPRREG
                     VGTQSSLYWVIGGFIRARQCIVGLREVRGADGILRCGIELDPGLVATEPVPRRPFQGW
                     RYLPESDAPRDLDPNAPKLDGGMPPAMRAALTELRLL"
     gene            complement(1414..>2055)
                     /locus_tag="VNR65_02565"
     CDS             complement(1414..>2055)
                     /locus_tag="VNR65_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17588.1"
                     /translation="QRERALNRGEIGRYLVTFVDNHDSFWQPGGRISNGAPDEQVIAS
                     IGYLLCSLGTACIYYGTEQGFSGSGGDNQMREAMFDWSKPGRNLLNTDCKIYQEIAKI
                     ADVARANEPLRFGRMYFRQISGDGVNFGIPYGSTYTLAFSRILYPDEILVAYNVADAN
                     REDRVIVDAELHADGSTMTFLYGKSGTVPVETAPDGSRYVKLNLAPHQFVILS"
BASE COUNT          424 a          574 c          616 g          441 t
ORIGIN      
        1 tcggtcaggc gcgcggggcg catctcgtgg agaatgacgt tggctcctcg ttcagctgcc
       61 tgccaagccg cttccgaccc cgcaaggcct gctcctacca cgtggagtgt tcgctctttt
      121 gtcatggccg caaggtagga caaaaccctt gcacactcaa tgtgaagcca tgcgcttcag
      181 tgggaagtta cgggtgatct ctcgatttct ttgcggctgg ctgataatga gaggcatgac
      241 cgtccacatc ttgaaacttt gcgttggctg cgactccgtc gaggaactgg ccgattggca
      301 gcggaagcgt ctcgccacag ggcaggatct cgtccacgtc acgaggcaga cgccgcgccg
      361 ggagggggtc gggacgcagt cctcgcttta ttgggtgatc ggcgggttca tccgggcgag
      421 gcagtgtatc gttggccttc gggaggtgcg gggagccgat ggcatcttgc gctgtggaat
      481 cgagcttgac cccggccttg ttgccacgga gccggttccg cggcgtcctt ttcaggggtg
      541 gcgctatctg ccggaaagcg atgcgcccag ggacttggac ccgaacgctc ccaagctcga
      601 tggtggaatg cctccggcga tgcgcgccgc gcttactgaa ctccgcctgc tctagcgagg
      661 cgtgcgcaaa atcggaatcg gttgcggcgc aatttttctt aagcccgagg atgcgccgag
      721 gtgcattcgg cgcgacgatc acggtgacgc atgcgtcgcc aatctggtca tgccattttg
      781 gacggcgctt gccgcacgcc ttatggtaaa ccgttcgtcg ccgcgccgag cgttaatagg
      841 aaagtggtta acaggagcgc gccggattta tttcccgccg catgtatgct taaaaaatcg
      901 tctatccggc gcgaaaagtg gtccgccgtg cctttgagcg cgccgccgat gatagatcat
      961 aacccattga aaacgaacag cgttttatcc ttaacagatt gcccattaaa tagaatttaa
     1021 tgccggaatt ttctcattaa ctgggcgagc aaaagtccgt taattatgtt tttggactat
     1081 ccagttaacg ccgcgcgcag ccggaactgc tgcggcagag gcgacggcga tggcgtcccg
     1141 ttcggtattt ttcatcgcaa ttatctcgct attatcaagg gttggagtgg gctggagctc
     1201 tcgttaacct gccttcaagg cgaattcgct gaagatcttt cagaacaaga agggcagacg
     1261 aaatcgtcag ggacaaattg acaagcagag caacatgcat acaaaaaatc atccagacga
     1321 ccgtgtggcg cgatgcttta gggcggatga gcgcctcaca agcggtcgag cgggcgcgcc
     1381 attcggacca gcatcttgcg gaggtcaacg gacctacgac aggatgacga actgatgcgg
     1441 cgccaggttg agcttgacat agcgcgagcc gtccggcgcc gtctcgacgg gaaccgtgcc
     1501 gctcttccca tacagaaacg tcatcgtgga gccatcggca tggagttccg cgtccacaat
     1561 gacgcggtcc tcacggtttg cgtctgccac gttgtaagca accaggatct cgtcagggta
     1621 cagaatgcga gagaacgcca gcgtgtaggt cgagccgtac gggattccaa aattaacccc
     1681 gtcgccagaa atctgacgga aatacatacg tccgaaccgt aagggttcgt tggcgcgcgc
     1741 cacatcggcg attttggcga tctcctggta aatcttgcag tctgtgttca acaggttgcg
     1801 gccgggcttt gaccagtcga acatcgcttc gcgcatctgg ttatcaccgc cgctcccgga
     1861 aaagccctgc tcggtgccat agtaaatgca cgccgtgcca agcgaacaca gcaggtatcc
     1921 gatagaagcg atgacctgct cgtcgggcgc cccgttgctg atgcggcctc caggttgcca
     1981 gaacgagtcg tggttatcca cgaaggtgac caggtagcgt ccgatctcgc cgcggttcaa
     2041 tgctcgttcc cgctg
//
LOCUS       DATMGX010000158         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_247507, whole genome shotgun sequence.
ACCESSION   DATMGX010000158 DATMGX010000000
VERSION     DATMGX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247507"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VNR65_02570"
     CDS             <1..432
                     /locus_tag="VNR65_02570"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWJ17589.1"
                     /translation="GKDLDSRQAAISSLARLQKDNKEITEQIAGYLAEPHAQIRFSSL
                     FALGTRGDASAIPALEALLKRDDLSIEMAPMIKQQIAGLKKPKPKEAAPAAMGEEGDG
                     EEDMGSSPSGDDQKLSHLEQLVQEMNERLKAIEGRLPPAKP"
     gene            complement(466..1779)
                     /locus_tag="VNR65_02575"
     CDS             complement(466..1779)
                     /locus_tag="VNR65_02575"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HWJ17590.1"
                     /translation="MPDAKIIARRIFQHTLASIDIPLVTQSKLRFEKNRLVLPDTSVD
                     LAHVSNFFVVAIGKAAHAMVDGLFAILPKGISCIGVVSAPIPCSNPWPGMVYFLSGHP
                     IPNDDSWNAAGEILRLLHSCDEHTLVFFLLSGGGSALVELPLDPAMQLMDVQELNRTL
                     VTCGAPIEAINTVRKHLSAIKGGRLAVAAAASRKITLAVTDVPAGKEWALASGPTLPD
                     PTKVADVEGIVAEYDLRKKLPSPLLRWLDAGKMPETPKDSHPAFANAHFVLLLGMHDL
                     FHAAHHAAEAQGYLTRCDNSTDDWPIDKAVDSLLAQLEEWQSDNPGRCVALIADGEVA
                     SPVTGKGIGGRNAAFVLACLEKIAGRKITVLSAGTDGIDGNSPAAGAVADGNSLARAS
                     AFGMDPSDAYKQSDAFSFFNKLEDAILTGPTNNNLRDLRIFLAEP"
     gene            complement(1798..>2064)
                     /locus_tag="VNR65_02580"
     CDS             complement(1798..>2064)
                     /locus_tag="VNR65_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ17591.1"
                     /translation="RGPVVDEAALVAALEANKIAGAALDVYENEPFIHPGLKLPNVVL
                     APHLASASIETRTKMAVMAATNVISLFKGQMPPNMINPAVLKTS"
BASE COUNT          495 a          527 c          644 g          398 t
ORIGIN      
        1 ggcaaggatt tggattcgcg gcaagcggcg atcagcagtc tggcacgctt gcaaaaagac
       61 aataaggaaa tcaccgagca gatcgccgga tatctggcgg agccgcacgc gcagattcgc
      121 ttctcatctt tgtttgcact gggcacccga ggggatgctt cggcgatacc ggcgctggag
      181 gcgctgctca agcgagacga tctgagcatc gaaatggcgc cgatgatcaa gcagcagatt
      241 gccggattaa agaagccaaa accgaaagaa gctgcgcccg cagcgatggg cgaagaggga
      301 gatggcgaag aagatatggg atcgagtccg agtggagacg atcagaaatt gagccacctg
      361 gagcagctag tgcaagaaat gaacgagcgg ttgaaggcca tcgaaggaag attgccgcca
      421 gctaaaccgt gagcgacggg gtcttatcgc tgaatcaaag acgaatcagg gctcggccag
      481 gaagatgcgg agatcgcgga gattgttgtt ggtcggcccg gtcagaatgg catcctcgag
      541 tttgttgaaa aacgaaaaag catcgctctg tttatatgcg tcggacgggt ccatcccgaa
      601 ggcgcttgcc cgcgccaggg aattgccatc cgcgacagcg cctgcggcgg gactgttgcc
      661 gtcgatgcca tccgtgcccg cgcttaagac cgtgattttg cgcccggcaa ttttttcgag
      721 acaggcaagg acgaaagcgg catttctgcc accaattccc ttccctgtaa cgggagaagc
      781 aacttcacca tcagcgatga gggcaacgca ccggccggga ttgtcactct gccactcttc
      841 caattgagcc agcagagaat ccacggcttt gtcgatcggc caatcatccg tggaattatc
      901 gcagcgcgtc aggtagcctt gcgcttcggc ggcatgatga gcggcgtgaa aaagatcgtg
      961 catgccgagc agcaaaacaa aatgagcgtt agcaaaggcg ggatgtgagt ctttgggagt
     1021 ttccggcatt ttccctgcgt ctagccagcg caatagcggg gagggaagct tttttcgcaa
     1081 gtcgtactcg gcaacaattc cctcaacgtc ggcaaccttg gtgggatcgg gcagggttgg
     1141 gccagaggcc agcgcccact ccttgcctgc aggaacatcc gttaccgcca gggtgatttt
     1201 cctggacgcg gccgccgcga cagctaagcg gccgcctttt atggcggaaa gatgcttccg
     1261 gacggtgttg atggcttcga tgggcgcgcc acaagtgacc agagtgcgat tcaattcttg
     1321 cacgtccatc aactgcatcg cggggtcgag aggcagctcc acgagcgctg acccgccacc
     1381 ggatagcagg aagaaaacta aggtgtgttc atcgcaggag tgcaatagac gaagaatctc
     1441 acccgcggcg ttccaactgt cgtcattcgg aatggggtga ccggaaagaa aatagaccat
     1501 tcccggccag gggtttgagc aaggaatggg agctgagacg acaccaatgc aggaaatgcc
     1561 tttgggaagg atggcgaaca atccgtccac catggcgtgc gcggctttac cgatggcgac
     1621 gacgaagaag ttcgaaacat gcgccaggtc aaccgacgtg tctggcagca cgagtctatt
     1681 tttctcgaag cgaagcttgg actgagtgac cagaggaata tcaatcgatg ccagagtatg
     1741 ttggaatatt cggcgcgcga tgatttttgc gtctggcatt gcggcacgac ccggaggtca
     1801 ggatgtcttc aggaccgcag gatttatcat gttcgggggc atctgccctt taaacaagga
     1861 gatgacatta gtggcggcca tcaccgccat tttggtgcgc gtctcaatgg acgcggaagc
     1921 caaatgggga gcgagaacaa cgttgggaag cttcagacca ggatgaatga atggttcgtt
     1981 ctcgtaaaca tcaagagcag cgccggcgat tttgttggcc tccagggcgg cgacgagggc
     2041 ggcctcgtcg accaccgggc cgcg
//
LOCUS       DATMGX010000159         2923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_247437, whole genome shotgun sequence.
ACCESSION   DATMGX010000159 DATMGX010000000
VERSION     DATMGX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247437"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VNR65_02585"
     CDS             complement(<1..212)
                     /locus_tag="VNR65_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17592.1"
                     /translation="MADVVPLLNEVERHARQDQWAVGMMAYEAAAAFDQAFQTLPPLE
                     GIPLAAFAIFDAPSEVEEEPLNAAFR"
     gene            complement(337..1368)
                     /locus_tag="VNR65_02590"
     CDS             complement(337..1368)
                     /locus_tag="VNR65_02590"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWJ17593.1"
                     /translation="MSYNVAVVGATGNVGREMLNILDEREFPVNEIFAIASRRSTGVE
                     VSFGEKTLRCQDLATFDFSKCDFALMSAGGDVSKEWAPKIGKMGCVVIDNSSAWRMDM
                     DVPLIVPEVNADAVVGFKKKNIIANPNCSTAQLVVALKPLHDAATIKRVVVATYQSVS
                     GAGKDAMDELWNQTKGIFVTDTPTPEQFTKQIAFNVIPHIDSFMEDGSTREEWKMMVE
                     TKKILDPKIKLTATCVRVPVFVGHSEAVNIEFEKPISAQEARDILREAPGLLVVDKPE
                     DGGYITPVECVGDFATYVSRIREDTTVENGLNLWVVSDNLRKGAALNTVQIAETLINR
                     GLIRKREAA"
     gene            1557..2345
                     /locus_tag="VNR65_02595"
     CDS             1557..2345
                     /locus_tag="VNR65_02595"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HWJ17594.1"
                     /translation="MLKPLEVTAAIWRIGMGRFTIESFIEEANSAKSTEEVARLFGEV
                     LKDYGYDRFCYSLLTDHPSLGLEAGHGIVANYPEDWMSHYKANLYEKKDPVPRHGFET
                     LRPFTWESIQQTRELKADEKRVMSEAREAGLLDGVAIPICGHNGELAGVGMASSIGGI
                     RPDVTVLRKLQALTMQFHLAFTDLEKKDRAVGIVHLTNREKEILLWVAEGKSDSIIAD
                     IIGVSHAAVRFHLNNIFKKLGANERTLATVKALRLGLILPSYVR"
     gene            2541..>2923
                     /locus_tag="VNR65_02600"
     CDS             2541..>2923
                     /locus_tag="VNR65_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17595.1"
                     /translation="MKTGFATLANKAADALMLGTLLPLLLKQVIMARDFAASAAGSVL
                     HAVVRESAVVRFAGDSGDGMQLSGNQFTLATALAGNALATFPDFPAEIRAPQGSTFGV
                     SAFQINFGSTSIETAGDEPDVLIAM"
BASE COUNT          642 a          828 c          799 g          654 t
ORIGIN      
        1 catctaaaag ctgcgttcag tggctcttcc tcaacctccg atggcgcatc gaaaatcgcg
       61 aaggccgcta gaggaatgcc ttccagcggc gggagcgttt gaaaggcttg atcaaaggcg
      121 gccgcagctt cgtaggccat catgcctacg gcccattggt cttggcgagc atggcgctcg
      181 acctcgttca aaagcggaac gacgtcggcc atgtgatgcg ccgttagaat ggcgagcgga
      241 tttctgaaac tatgccgcaa ccgcgaggcg ttatcgcgcg gaaagtcgat cgtgcagcga
      301 atgggcaggg ccggtcccga gggcgaagag ggcggcctaa gccgcctccc gtttccggat
      361 cagtccgcga ttgatgagcg tctccgcaat ctggacggta ttgagcgcag cccccttgcg
      421 aagattgtcc gagaccaccc aaaggttcag gccattctcg acagtcgtat cttcgcggat
      481 gcgactcaca taagtggcaa aatcgcccac gcactcgacc ggcgtgatgt agccgccatc
      541 ctcgggctta tcgacgacga gcagtcctgg cgcctcgcgc agaatgtccc gcgcctcctg
      601 ggccgaaatc ggcttttcga actcgatatt caccgcctcg gaatgcccca cgaagaccgg
      661 cacgcgcacg catgttgccg taagcttgat cttggggtcg agaattttct tggtctcgac
      721 catcatcttc cactcttcgc gtgtggagcc gtcctccata aaactgtcga tgtgcggaat
      781 cacgttgaac gcaatctgct tcgtgaactg ctcgggcgtg ggggtgtcag tgacaaagat
      841 tcccttcgtc tggttccaca actcgtccat tgcgtccttg ccggcgcccg aaacggactg
      901 ataggtggcg accacaacgc gcttaatcgt cgccgcatcg tggagcggct ttagcgcgac
      961 gacaagctgt gcggtggagc aattcgggtt cgcgatgata tttttcttct tgaagcccac
     1021 gacggcgtcg gcgttcacct cgggcacaat cagcggcaca tccatatcca tgcgccatgc
     1081 gctcgaatta tcgatgacga cgcagcccat tttgccgatt ttcggcgccc actctttcga
     1141 cacgtcgccg cccgccgaca tcagcgcgaa atcgcatttc gaaaagtcga acgtcgcaag
     1201 gtcctgacac ctcagcgtct tctcgccaaa cgaaacttca acgccggtcg aacggcgtga
     1261 ggcgatggcg aaaatttcat tcacggggaa ttcgcgctcg tccaggatgt tcagcatttc
     1321 gcggcccacg ttacccgtgg cgccgacgac agcgacgtta tagctcatga cggtgtccta
     1381 tttctgagat cgccggaatg taagcgcggc tggaacgcat gtccactgca acactcgtcg
     1441 cctttgtcgc gtgtttaggc gacgtaaagt gtatacagtt gtgtgtgcgt acacgcccct
     1501 cacgccgttt tgccgttgcg cgaggctcgt ggcatgttaa taggggatag gaatagatgc
     1561 ttaagccgct ggaagtgacg gcggctatct ggcgaatagg aatgggaaga ttcacaattg
     1621 aatccttcat cgaagaagcc aacagcgcca aatcaactga agaagtagct cggcttttcg
     1681 gtgaagtcct caaggattac ggatatgatc ggttctgtta tagcctttta acagatcatc
     1741 cttcgcttgg cttggaagca ggtcacggaa ttgttgcaaa ttatcctgaa gattggatgt
     1801 cgcattacaa agccaacctt tacgaaaaaa aggatccggt tccgcggcac ggttttgaaa
     1861 cgctgcgccc attcacgtgg gagtcgatcc aacagacccg cgagctcaaa gctgacgaaa
     1921 agagggtcat gagcgaagcg agagaggctg gacttctaga cggcgtcgcc atcccaatct
     1981 gcggccataa tggcgagttg gcgggggtcg gcatggcaag cagcattggc ggaattcggc
     2041 ccgatgtaac agttttaaga aagctgcaag ccctcaccat gcagtttcat ctggcattta
     2101 cagatttgga gaaaaaggac cgggccgtgg gaatcgttca tctcaccaat cgggaaaaag
     2161 aaattctgtt gtgggtcgct gaagggaaaa gcgattccat catagccgac attattggcg
     2221 tttcgcacgc tgcggtccgt tttcacttga ataatatttt caagaagctc ggtgcaaacg
     2281 agcggacttt agcgaccgta aaggccctcc ggctcggtct cattctccca tcctacgtca
     2341 ggtaaaagaa cgccggcgcc aggggccaag cttttatacc gcaagctcgg ccaagtgcat
     2401 tgcatcaact ccgtttcgac acgtcggcta ttgctgcatc cgaggcttca gcctcgcgga
     2461 tgccccctct tcagtatcac ttggcgaaca acaccttgcg gtcctcatca aagctggcta
     2521 tgacctaagc cttcctcatc atgaagaccg gctttgccac cctggccaac aaggcagccg
     2581 acgcgttgat gctgggaaca cttttgccgc tcctattaaa gcaggttatc atggctcgag
     2641 atttcgccgc ttctgcagct ggttccgtac tccacgccgt ggtgcgtgaa tcagctgtcg
     2701 tccgcttcgc gggtgactca ggcgacggca tgcaattgtc tggaaaccaa tttacgctcg
     2761 ctaccgctct cgcgggcaac gcactcgcca cgtttccaga ttttccggct gaaattcgtg
     2821 cgccccaggg atcgaccttc ggcgtatcag cctttcaaat caattttggc tcgacctcca
     2881 tagaaaccgc cggtgatgaa cccgacgtgc tgattgcgat gaa
//
LOCUS       DATMGX010000160         2907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_316086, whole genome shotgun sequence.
ACCESSION   DATMGX010000160 DATMGX010000000
VERSION     DATMGX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316086"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..>2907
                     /locus_tag="VNR65_02605"
     CDS             109..>2907
                     /locus_tag="VNR65_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWJ17596.1"
                     /translation="MSISAPFIARPIATSLLAVAILLSSALAYSFLPISSLPQVDYPV
                     IQVTTQLPGANADTMARLVTAPLERQLGQIPSLEGMSSTSSQGVSQITLQFNLDRDIN
                     AAGQDVQSAISAAGGTLPQNLPYPPVYAKVNPSDPPIMTIALTSQSLSMERLSDLADT
                     LLSPRLSQISGVGRVTIQGNIRPAIRIQANPLQLSSYGLALETLRAAIANANVTGSKG
                     VLSGSDKAYLIGANDQLETAKAYEDIVVAYNNGAPVLLRDVATVIAGLENNKVLARYN
                     GLPAVVIDIQRQPGANIVGTVDRIKKVLPKLEAALPAGVKAEVVADRTGTIRASVHDV
                     QVTLILSVVLVVMVVLLFLRTLSATIVAGVTLPLSLIASFGIMYFAGFSLDNLSLMAL
                     TIATGFVVDDAIVMIENIMRYIEKGETPMAAAYKGAGEIGFTIVSLTVSLIAVFIPLL
                     FMTGIVGRLFREFALTLTIAVLTSMVISLTLTPMMSARLLRVPRHGNGAPWYARLAEA
                     PITALTAFYRTTLDIVLRFRTLTLIVAALTLVLTITMYLAIPKGFLPDQDTGFLTAET
                     ETAPDVSFDRMNVLQNSVEKIILKDPDVLGVVSVVGVGTTNATPNVAHFALTLKPKND
                     RTASSTAIMQRLTAATSGLPGISTTFQIVQDIQIGTARSRTGYQYVLVGLDRDGFSSW
                     AKKLEAELKRDLRLIHVSSDIQEDGNAVMIKTDRVIAGRLGVTMQALNDTLYDAFGQR
                     QVSTIYGQSNQYRVILEVDPKFQTDLAALGSIYVPGNAISNSTTGNSVTGLSSGSSGP
                     TGSSITAVPTIGQGRGAQVLLSSFATIERTIAPLSVNHVEQYPAATISFDVAPGVSLD
                     AAIQAVAEATARLGMPSSIVGSYSGAAAEFNTSLKSTPLLVLAAVVTIYIILGVLYES
                     FIHPFTILTTLPSAGIGAL"
BASE COUNT          545 a          925 c          847 g          590 t
ORIGIN      
        1 agaggcgtcg gcggatgggg atgctgcggg cggggagcaa gccgggactt ggaaaaagcc
       61 cgtgcagggg caggccgaag ctgccaccac cgagggcacg cgccggccat gagcatttcc
      121 gcgccgttca tcgcgcgccc gattgccact tcgctgctcg ccgtcgcaat cctgctctcg
      181 agcgcgctcg cttattcatt cctgccgata tcctcgcttc cgcaggtcga ctatcccgtc
      241 atccaggtga cgacgcagct tcctggcgca aacgcggaca cgatggcgcg gctcgtaacc
      301 gcgccgcttg agcgccagct tgggcaaatc ccctcgcttg aaggcatgag ctcaaccagc
      361 tcgcagggtg tgagccagat cacgctgcaa ttcaacctcg accgcgacat caacgccgca
      421 gggcaggacg tgcagtctgc gatcagtgcc gcgggcggca cgctgccgca aaatttgccc
      481 tatccgccag tttacgcgaa ggtcaatccg tccgatccgc cgattatgac catcgcgctt
      541 acatcgcaaa gcctttccat ggaaaggctg agcgatctcg ccgacacgct tttgagcccg
      601 aggctcagtc aaatttcggg tgtgggccgt gtcacgatcc agggcaatat ccggcccgcg
      661 atccgtatcc aggccaaccc gctgcagctt tcctcctatg ggcttgcgct tgagacgttg
      721 cgcgccgcca tcgccaacgc caacgtcacc ggatcgaaag gcgttctcag cggatcggac
      781 aaagcctacc tcatcggtgc caatgatcag ctcgaaaccg ccaaggccta tgaagacatc
      841 gtggtggcct acaataacgg cgcgcctgta ttgctgcggg acgtggctac ggtcatcgcg
      901 gggcttgaga acaacaaggt gctggcgcgg tataacggtt tgccggctgt cgttatcgac
      961 atccagcgcc agccgggcgc gaacatcgtc ggcaccgtcg accgcatcaa gaaagtcctg
     1021 cccaagcttg aagccgcgct tccggcgggc gtgaaggcgg aagtcgtcgc agaccgcaca
     1081 ggcaccatcc gcgcgtccgt gcacgatgtg caggtcacgc tgatcctgag cgtcgtcctt
     1141 gtggtgatgg tcgtgctttt gttcctgcgg acactgagcg caaccatcgt ggcgggcgtg
     1201 acgctgccgc tgtccttgat cgcctctttc ggcatcatgt atttcgcggg cttcagcctg
     1261 gataacctct cgctcatggc gctgaccatt gcgacgggct tcgtcgtcga cgacgccatc
     1321 gtgatgatcg aaaacatcat gcgctacatc gagaagggcg agacaccgat ggccgctgcc
     1381 tacaagggcg cgggcgagat cgggtttacg atcgtgtcgc tgaccgtgtc gctcattgcg
     1441 gtgttcatcc ctcttctgtt tatgaccggc attgtcgggc gtcttttccg cgagttcgct
     1501 ctgacgctga ccatcgcggt gcttacctcg atggtgatct cgctgacgct gacgccgatg
     1561 atgagcgcgc ggcttttgcg ggttccccgt catggaaacg gagcgccctg gtatgcaaga
     1621 ctcgctgaag ctccgatcac cgcgcttacc gcattctacc ggaccacgct cgatattgtg
     1681 ctgcggttcc ggacgctgac gctgattgtg gccgcgctga cgctggtctt gacgatcaca
     1741 atgtatctgg ccattccgaa aggcttcctc cctgaccagg atacgggctt cctgacggca
     1801 gaaacggaaa ccgctccgga cgtgtccttc gaccggatga atgtgcttca aaacagcgtc
     1861 gagaagatca tcctcaaaga cccggatgtg cttggtgttg tatccgtggt cggggtcgga
     1921 acgacgaatg caacgccgaa cgttgcgcat ttcgcgctga ccttgaagcc gaagaacgac
     1981 cgcaccgcaa gctcaaccgc gatcatgcag cggctcacag cggcgacgag cggcctcccc
     2041 ggcatttcga caacatttca gatcgtccag gatattcaga tcgggacggc acggagccgc
     2101 acggggtatc aatacgtgct cgttgggctc gaccgcgacg gcttctccag ttgggcgaag
     2161 aagctcgaag ccgagctgaa gcgcgacctc cggctaattc acgtttcatc cgacattcag
     2221 gaagacggca acgccgtgat gatcaagacc gaccgcgtca ttgcgggcag gctcggcgtc
     2281 acgatgcaag cgctgaacga tacgctctac gatgctttcg ggcagcggca ggtttcgacc
     2341 atctatggtc agtcgaacca gtatcgcgtc attctcgaag tcgatccgaa gttccagacg
     2401 gatcttgcgg cgctggggtc catctacgtc cctggtaatg cgatcagtaa ctccacgacc
     2461 ggcaattcgg ttaccggtct ttcgagcggg agcagtggcc cgaccggttc ttccatcacc
     2521 gccgtgccca ccatcgggca gggtcgcggt gcgcaagttc tactttcatc cttcgcaacg
     2581 atcgagcgaa ccattgcgcc gctgtccgtc aaccacgtcg agcaataccc cgccgccacc
     2641 atcagcttcg atgtcgcgcc cggcgtttct ctcgatgccg ccattcaggc cgtggccgag
     2701 gcgaccgccc gccttggcat gcccagctcg attgtcggca gctatagcgg cgccgcggcc
     2761 gagttcaaca cctcgcttaa gagtacgccc ttactcgtgc tcgccgcagt tgtgacgatc
     2821 tacatcattc tcggcgtgct gtatgaaagc ttcattcacc cctttaccat tctcacgacg
     2881 cttccgtccg cgggcatcgg cgcgctg
//
LOCUS       DATMGX010000161         3318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_315940, whole genome shotgun sequence.
ACCESSION   DATMGX010000161 DATMGX010000000
VERSION     DATMGX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315940"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VNR65_02610"
     CDS             <1..1105
                     /locus_tag="VNR65_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HWJ17597.1"
                     /translation="TIAERIRTDTGGRGIRVSSSQEQIVLTGEAANSVEAEHALEIAK
                     SVSPETAVINLMKIAPSQQVLLKVRFLEASRSAERDVGVNLFGANKAGTSGVNTGVST
                     LNGSTAPGLPLLQTLAALTGGSGSTPFVTAIGSIGNVDVLISALEQKGLVRELAEPDL
                     VALSGDTASFLAGGEVPIPVVQPSSGATPTITVDYKQFGIQLTFQPTVLANGIINLRL
                     TPSVSELDYANAIQNQGFLIPALKKREARTTIELRDGQTFAIAGLLQSEGLRNISQVP
                     WIGSVPVLGTLFRSSAYQQKETDLVVIVTPHIVAPIVPGQRVASPLDHRLPSNDMDFF
                     LLGQTDVPKKYTEYVTSGGGLAGPYGHIVPLGH"
     gene            1131..1469
                     /locus_tag="VNR65_02615"
     CDS             1131..1469
                     /locus_tag="VNR65_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17598.1"
                     /translation="MLKLSSFSALAFAAAASAALAGDQSFEDFFGSYFHRTDTIAVGA
                     GDAKNVNAASEMLDPWPPYVRNRSLPGNGSRMTGAVQRYEDVRKLKEAPPTLTPESIN
                     PIGFSASQSK"
     gene            1553..2041
                     /locus_tag="VNR65_02620"
     CDS             1553..2041
                     /locus_tag="VNR65_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ17599.1"
                     /translation="MVALMLSGCGAIDNTSAPTTLTVQEPASEKYFPSDEPYRLGAEF
                     FNRGDYGLAERYFRDAVEKAPEDGGAWAALASSYDQLRRFDLADRAYQRAIALCGETP
                     QILNNQGYSQMLRGNLKSARRKFLQAATLDPGNPTILNNIRLLDGSQPYIGPSPWGTH
                     IQ"
     gene            complement(2070..3008)
                     /locus_tag="VNR65_02625"
     CDS             complement(2070..3008)
                     /locus_tag="VNR65_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWJ17600.1"
                     /translation="MPHPFVSHLGSFGRLAPFLAMVPILFGGAVLIFSSMRLRTRTIA
                     RRVEMVKFRSASRDLAPEHSAPSDGHQFEDGTQGLSVPEQRQIARFFAKYNVEPGPAI
                     LYFTLLRLSAIAVLASLAYLAGHKTTWPLPLLIATVAATAGWFIPIMFVRVALKNYRK
                     AVALGLPDAIELLAICADAGVSLESGLQRVSRELRHTQPALAGELALTWAQISIFPNR
                     DQALLNLADRIDLPSLRSVVSTLSQSMRFGTPLAQSLRTAAIEMRNDLLLRLEERANR
                     LPGLLTFPVMLLIMPTIFLIVGGPAVIKIMDTFKSG"
     gene            complement(3076..>3318)
                     /locus_tag="VNR65_02630"
     CDS             complement(3076..>3318)
                     /locus_tag="VNR65_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17601.1"
                     /translation="RNRRATQLKARAATAEVRFSAYVLGALPFVTVAALLVVSPGYLT
                     PLFHDSRGHVILAAAAVGLLVSGLVMRQMMRSVEQP"
BASE COUNT          712 a          956 c         1019 g          631 t
ORIGIN      
        1 aacgattgcg gagaggatcc gcaccgatac aggaggccgc ggcatccgtg tgtcgagcag
       61 ccaggaacaa atcgtcctga ccggagaagc cgccaattcg gtcgaagccg agcatgccct
      121 ggaaatcgcc aaaagcgtgt ctccagaaac ggcggtcatc aacctgatga agatcgcacc
      181 cagccagcag gtgttgctca aggtgcgctt tctcgaggcc agccgcagcg cggaacgcga
      241 tgtcggcgtc aaccttttcg gcgctaacaa agctggaacg agcggcgtca atacgggggt
      301 gagcacactc aatgggtcaa ccgcgccggg gctccctttg ctgcaaacac ttgcagcgct
      361 gaccggcggg tcgggttcca cgcccttcgt gacagcaatc ggcagcatcg gcaatgttga
      421 cgttctgatt tccgctctgg aacagaaggg gctcgtgcgg gaactggctg agcccgatct
      481 cgtcgccctg tcaggcgata cggcaagctt cctggccggc ggcgaggttc cgatacccgt
      541 tgtccagccg tcctccggtg cgacgccgac aattacagtc gactacaagc agttcggcat
      601 tcagctcacc ttccagccga ctgtgctcgc aaatggcatc atcaatcttc ggctcacgcc
      661 gtcggtcagc gaactggatt atgcaaatgc gatccagaac cagggcttcc tgatcccggc
      721 cctgaagaag agagaagccc gcacaacgat agagctgcgc gacgggcaaa cctttgcgat
      781 tgccggcctg ctgcaaagcg aaggccttcg gaacatctcc caggttccgt ggatcggctc
      841 ggttcctgtg ctcgggacgc tgttccgcag ttcggcttac cagcagaaag aaacggatct
      901 ggttgtcatc gtcaccccgc acatcgttgc gcccattgtg ccggggcagc gggttgcaag
      961 ccctctcgac catcgtctgc cgagcaatga catggatttc ttcctgcttg gccagacgga
     1021 tgtgccgaag aaatacaccg agtatgtgac gtcaggcggc ggtctggcgg gtccttacgg
     1081 tcatattgtt ccgctgggtc attagctccg agcgaaaaaa ggtgttgcgc atgttaaagt
     1141 taagcagttt tagcgctttg gcctttgcag cggctgcctc ggccgctctg gcgggcgatc
     1201 agagtttcga ggacttcttc ggctcctatt tccatcgaac cgataccatc gcagtcggcg
     1261 cgggcgacgc caagaatgtg aacgcggcgt cggaaatgtt ggacccttgg ccgccctatg
     1321 tcaggaaccg aagcctgccc ggcaatggtt cgcgcatgac gggagccgtt caacgctatg
     1381 aggatgtgcg caagctgaag gaggctccgc cgaccttgac tcccgaatcc ataaacccga
     1441 tcggattttc cgccagtcag tcgaagtgat ggaggcgcgt gtgaccatgg accggtctca
     1501 agctctcagc cgggttaggg gtcgccttcc gtcttggagg cgagccttcg tcgtggtggc
     1561 tcttatgctc tcgggatgcg gagcaatcga caacaccagt gcccccacga cgctcaccgt
     1621 gcaggagcca gccagcgaga aatactttcc atccgacgag ccttaccgcc tgggggcgga
     1681 gtttttcaat cgcggcgatt acgggcttgc ggaacgctac ttccgcgatg ccgtggagaa
     1741 ggcgccagag gacggcggag cgtgggcggc gctcgcttcc agttacgacc agctgaggcg
     1801 tttcgatctt gcggatcgcg cctatcagcg cgcaatcgcg ctctgcgggg agacgcccca
     1861 gattttgaac aatcaaggat actcccagat gttgcggggg aatctcaaat ccgcgcgccg
     1921 gaagtttctc caggctgcca ccctcgatcc agggaacccg acaatcctga acaatataag
     1981 gctgttggac ggcagccagc cctatatagg gccttcccct tggggcactc acattcaata
     2041 aggggatggc tcggtgaggc gtcccggagt tacccggatt tgaaggtatc catgatcttg
     2101 atgacagctg gaccgccgac aatcaggaag atcgtgggca taatcagtag catgacggga
     2161 aatgtcagga gccccggcaa gcggttggcg cgttcctcca accggagcag caggtcgttt
     2221 cgcatctcga tggcggccgt gcgcaacgac tgtgcgagag gcgtaccaaa acgcatgctc
     2281 tgagacagcg tgctgacaac cgatcgcaaa cttgggagat caatgcgatc cgccaggtta
     2341 agcagcgcct gatctcgatt tggaaagatg ctgatctgtg cccaggtgag cgccagttcg
     2401 cccgccaaag ccggttgcgt atggcgcagt tcgcgcgaga ctcgctgaag gccgctttcg
     2461 agagaaacgc cggcgtcagc gcatatagct aagagttcta tcgcatccgg gaggcctaac
     2521 gcgaccgctt tgcggtagtt tttcagagcg accctgacaa acataatggg aatgaaccat
     2581 ccggccgtgg cggcgaccgt ggctatcagc aaaggcagcg gccacgttgt cttgtggccg
     2641 gcaagatagg cgagagaggc aaggacggcg attgcactca atcttaagag cgtgaagtat
     2701 aatatcgcgg gaccgggctc gacattatac ttggcgaaga accgggcgat ctgccgctgc
     2761 tccggcacgg aaaggccttg cgttccatct tcgaactgat ggccgtcgct cggagcggaa
     2821 tgctcgggcg ctaagtccct gctggcagac cggaatttca ccatttccac acgccgtgca
     2881 atcgtgcgcg ttcgaagccg catgctggaa aagatcagga ccgcgccgcc aaatagaatg
     2941 gggaccattg ccaggaaggg ggcaaggcgg ccgaaagagc cgagatggga tacaaaggga
     3001 tgaggcatcg tgcgtcctcc tcagatcaac ccgcccttac tcggaatcgc ttaaaggcgg
     3061 ataagctgct ctagatcatg gctgttcgac gcttcgcatc atttgcctca tcacaaggcc
     3121 agaaaccaga aggcccactg cggcggccgc gaggatgaca tggccgcggg agtcatggaa
     3181 aagaggcgtg aggtagccgg gcgaaacgac gaggagcgcc gctaccgtca caaatggcag
     3241 cgcgcctagc acataggcgg aaaaacgtac ttccgcggtc gcggcacggg ccttcagctg
     3301 tgtggcgcgt cggtttcg
//
LOCUS       DATMGX010000162         2713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_63606, whole genome shotgun sequence.
ACCESSION   DATMGX010000162 DATMGX010000000
VERSION     DATMGX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63606"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VNR65_02635"
     CDS             complement(<1..704)
                     /locus_tag="VNR65_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWJ17602.1"
                     /translation="MSYSVLIVDDEALTVRTIGRALESEGFEVFLAMSGEEALTTISE
                     EKPDMALLDVVLPGINGIEVLRQAKKLSPATIIVMMSAYHVVDRAVEAMKLGAYDYLI
                     KPFHIADMVNTIRRATEMLALRVRVHDTVQTAKGRYDFGRVVTQSPVMREILEMARRA
                     AESDHTTILIQGESGTGKEVLAKAIHYNSSRAQAPLISLNCAALPDTLLESELFGYEP
                     GAFTDARRRKEGLIEKA"
     gene            complement(707..1795)
                     /locus_tag="VNR65_02640"
     CDS             complement(707..1795)
                     /locus_tag="VNR65_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ17603.1"
                     /translation="MVLSASLSDRLRAACRSVRHAPRLFVSVQAFVLFLIFSPKSYGF
                     SERPAAPNGGFWGGIVVGFVLLLVVAVLARTRIKAAEREALRSKELADHREYERNVAQ
                     QELLRRLEEERELAKEKMQFESQLSEYEKYASLAQLALGAAHEINNPLLGILSHLELE
                     WKDASGERREEIEQCIEGAKRISLAVRGLLDYARPGPLMLSKVNLSRLAAETLKFLEH
                     QPMFRQIELQNCIPLDLPNLSADANQLSQILMNLLLNAAQATPPGGTITILADKVKFA
                     EMIELRVRDTGSGIPADILPHVFEPFFTTKRGKGTGLGLSITQSYVRSHGGDIQLESI
                     PDLGTTVRVTLPIRQVGQAMQYSEEVIA"
     gene            complement(1992..2399)
                     /locus_tag="VNR65_02645"
     CDS             complement(1992..2399)
                     /locus_tag="VNR65_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17604.1"
                     /translation="MQWLERFRRSRLFLLGLGFLPLRLVWLGTMMLAMFAPLSLARHL
                     AAGWVLLIMAAIALIYFAGADLLHLARLGAYASLAEDDAHPVPAPEASNPPDLALAPE
                     PDSRLRLCRRLIRYKEAGEGVRKADPSRAKARS"
BASE COUNT          522 a          840 c          822 g          529 t
ORIGIN      
        1 gctttctcga tcagtccttc tttgcggcgg cgagcgtcgg tgaaggcgcc cggttcgtag
       61 ccgaatagtt cactctccag caaggtgtca ggcagggcgg cgcaattcag gctgatgagc
      121 ggcgcctggg cgcgcgagct gttgtagtgg atggccttgg ccagcacttc cttgccggtt
      181 ccactctcgc cttgaatgag gatggtggtg tggtcgctct ccgccgcccg gcgcgccatc
      241 tcgagaatct cgcgcatcac cgggctttgg gtcaccacgc gcccgaaatc gtaacggccc
      301 ttggcggtct gcaccgtgtc gtgaacgcgc acgcgcagcg ccagcatctc ggtcgcgcgc
      361 cggatggtgt tgaccatgtc cgcgatgtga aatggcttga tgaggtagtc gtaggcgcca
      421 agcttcatgg cttccacggc gcggtccacc acgtgatagg cgctcatcat cacgatgatg
      481 gtggccggag aaagcttctt ggcctggcgc aacacttcga tgccgttgat gcccggcagc
      541 acaacatcga gcaaagccat gtccggcttc tcttcggaga tggtcgtcag cgcttcctcg
      601 ccgctcatgg ccaggaaaac ttcgaagccc tcgctctcca gagcgcgccc aatcgtgcgc
      661 accgtgagcg cttcgtcatc gacaatgagg acggaatacg acatcgctag gcgatgacct
      721 cctcgctata ctgcatggct tggcccacct gtcgaatcgg cagggtcacg cggacggtag
      781 tgcccaggtc cggaatgctc tcgagctgga tatctccgcc gtgactgcgt acgtagcttt
      841 gcgtgatgct cagtccgagg cccgtccctt tgccgcgctt ggtagtgaag aacggctcga
      901 agacgtgagg cagaatgtca gcaggaattc cactgccagt gtcgcgcacg cgcagctcga
      961 tcatctccgc gaacttgacc ttgtcggcca ggatcgtgat cgtccctccc ggcggtgtcg
     1021 cctgcgcggc gttcagcagc aggttcatca ggatttgcga gagctgatta gcatcggcgc
     1081 tgagattcgg caggtcgaga ggaatgcagt tctgcaattc gatctgccgg aacatgggtt
     1141 ggtgctcgag aaatttcagg gtctcggcgg cgagccggct gagattgacc ttgctgagca
     1201 tcagcggccc gggacgcgca tagtccagca gtccgcgcac cgccaacgaa atccgtttgg
     1261 cgccttcaat gcactgttca atctcttccc ggcgttcgcc gctggcgtcc ttccactcca
     1321 attccaggtg cgacaggatt cccagtagcg ggttgttgat ctcgtgggcc gcgcccaagg
     1381 cgagctgtgc cagagacgcg tacttctcgt actccgacag ttgcgactca aactgcatct
     1441 tctccttcgc cagttcgcgc tcttcctcca ggcgacgcaa caattcctgt tgcgccacat
     1501 tacgctcgta ttcgcgatga tcggcgagtt ccttcgaccg gagagcttcc cgttccgccg
     1561 ccttgatccg ggtccttgcc aggacggcca cgacgagcaa cagaacaaat ccaaccacga
     1621 tgccgcccca gaatccgcca ttcggggccg cgggtctctc gctgaagccg tacgattttg
     1681 gggagaaaat gaggaacagg acgaaagcct gcacggacac aaagaggcgc ggggcgtgac
     1741 gcacactacg gcacgccgcc cgcaggcgat cgctcagcga ggctgagagc accatgcaac
     1801 tctcctacta tgacgttcgc gcagtattgc cgcagcagca gtgacagcag tcacctgccc
     1861 aggtgatggc tgaatgttcg gcagatggag cagcgggaaa ggccggtgtt atcaggctgc
     1921 tgaagatcat ccgggttcgg ccttgaaggg gcggggcttt agccacgccc ttgtgagccc
     1981 cttttttgtc atcacgaacg ggctttagcc cgtgagggat ctgcttttcg gactccttca
     2041 ccggcctctt tataccggat cagccgccgg cataatctca ggcggctgtc gggttctggc
     2101 gcgagcgcga gatcgggagg attgcttgcc tccggggcgg gcacgggatg cgcgtcatct
     2161 tccgccagcg acgcgtaggc tcccagacgc gcaagatgca gcaggtcagc tcccgcgaaa
     2221 tagatcaacg cgatcgctgc catgatgagc agcacccagc ctgctgcgag atgccgcgcc
     2281 aagctcagcg gcgcgaacat cgccagcatc attgtcccga gccataccag gcgaagcgga
     2341 agaaagccca gccccagcag gaacagccgg ctacgtcgaa aacgttctag ccattgcacc
     2401 caaccccgct cccagcggag tggagcatat ccaagaactc cagaagtagg gcaggcggaa
     2461 gtctcctggg gtccgaaact tcgcgcggat cgatcgcgcc tcccgccaca tgccccagta
     2521 gccccagacg caagctgttt tgaaggcgcg cacgctaaag aatcggtgta ggagggaacg
     2581 tagggattgc ggacagtccc gctctatgtc atccgcattg gaaagcaaag gctcaacggg
     2641 tctggcgccg cctaggttct ggctgagatt cccagcctcg cggtgaacac gcacgcacgg
     2701 ccgcatgtca aaa
//
LOCUS       DATMGX010000163         3282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_151419, whole genome shotgun sequence.
ACCESSION   DATMGX010000163 DATMGX010000000
VERSION     DATMGX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151419"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..940
                     /gene="bchJ"
                     /locus_tag="VNR65_02650"
     CDS             284..940
                     /gene="bchJ"
                     /locus_tag="VNR65_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriochlorophyll 4-vinyl reductase"
                     /protein_id="HWJ17605.1"
                     /translation="MAHASAEAGRIGPNAIIRVAETLRDVLGEETTRLIFARAKLEPH
                     LAHPPTGMVPEEDVGALQTALFDGLEESEARKIAFEAGLRTGDYLLANRIPKPAQAVL
                     KVLPSSLASRILLGTIKKHSWTFSGTGDFSVANGHPVIVSIKGCPLCRGRHGDHPQCD
                     YYAGTFQRLFEVLVSRNTFVREVTCEATGGASCAFEIAWKNRSRGGGSQRILTGKEQS
                     "
     gene            937..2289
                     /gene="hemN"
                     /locus_tag="VNR65_02655"
     CDS             937..2289
                     /gene="hemN"
                     /locus_tag="VNR65_02655"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HWJ17606.1"
                     /translation="MIPGIAKYFSARVPRYTSYPTAPHFSADVDAKRYETWLSRLDPS
                     SEISLYLHVPFCRQMCWYCGCNMRVIARYSPVTTYVDTIIREIELAARVMPQGISVRH
                     IHWGGGTPTALAPEDMIRIAETVQRHFNILGTAEIAVEIDPRTFTRENAVALGKIGCT
                     RASLGVQEFDEKVQTAINRVQPISMIESTLTLLREQGITEVNFDLMYGLPYQTAETLK
                     DTVEQAAKLNPARIALFGYAHVPWMAKNQKMIPEESLPTPTMRFEQAQVAGDALAEQG
                     YVKIGLDHFARPEDSMAVALREGCLHRNFQGYTNDEAGTIVAFGASAISALPEGFAQN
                     IVETGAYTRAVNSGVLPIAKGIVFRGEDKMRGAIIERLMCEMEVDLEDIRKQYAPDRS
                     GFSDEMSQLRGLEEEGLCTLEGSRVRVPDEARLALRVVCSVFDAYLAQSTELRHAAAV
                     "
     gene            2439..2753
                     /locus_tag="VNR65_02660"
     CDS             2439..2753
                     /locus_tag="VNR65_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17607.1"
                     /translation="MARIFIFLLFGIAFCYGARAEEGSAEAGLTYARTICSECHAIRS
                     DDVLSPNPKAPPFAKIASTSGMTGAALFVILQTPHRDMPDLIIPAQDKADVVAYILSL
                     QK"
     gene            2876..>3282
                     /locus_tag="VNR65_02665"
     CDS             2876..>3282
                     /locus_tag="VNR65_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWJ17608.1"
                     /translation="MDRNRLSPLLLLPNLLSMFRLAAAPVLAWAVLASQTPALAIGIV
                     IAAAMTDLLDGHCARATGQVSEVGAVLDPIADKIFILTALSLLWGESIIRGANAWAVL
                     IILWREVLISGLRDYARFEGHQAPVSRVAKFKTA"
BASE COUNT          698 a          977 c          934 g          673 t
ORIGIN      
        1 cgcgtgaaat attggggtcc acagtctctc aagaaggtgg atttcggctt cgataccaag
       61 cgtgaacggg gcgagcgcga agaggacgac ttcgaccata cgacgcggaa gaccggccac
      121 cacgaagaga aaaaggtcga aaccgcgacc acgcactaga acagcccgca tccctccggc
      181 gcggacaagc tgctctagct ccttagaatc gatcaacccg ccttcgattc cgattaaagg
      241 cgggttgatc tagaccctgt cacagacgaa gaaggcgctt cgaatggcgc atgcatcggc
      301 ggaagccggc cgcattggtc caaatgctat aatccgggtg gcggaaaccc tacgcgacgt
      361 tttgggtgaa gaaacaaccc ggcttatttt cgcccgcgcc aaattagagc cgcatcttgc
      421 tcatccaccg acggggatgg ttccggagga ggatgtcggc gcgcttcaaa cggcgctttt
      481 cgacggactt gaggagagcg aggcgagaaa gatcgcgttc gaggccgggc ttcgaaccgg
      541 ggactatctc ctcgcgaacc gcataccaaa gcctgcccag gcggtgctga aggttctgcc
      601 atcctctctc gcgagcagga ttctgctcgg cacaatcaag aagcactcct ggaccttctc
      661 cgggaccggc gacttcagcg tcgcaaacgg gcatccggtg attgtttcga tcaagggctg
      721 cccgctttgc cgagggcgcc atggcgatca tccgcaatgc gactattacg caggaacctt
      781 ccagcgcctc tttgaagtcc tcgtcagccg caacactttc gtccgagaag tgacctgcga
      841 ggcaacaggc ggcgcctctt gcgcattcga aatcgcatgg aaaaatcgaa gccgcggcgg
      901 cggctcgcag cgtatcctta cagggaaaga acaatcatga tacctgggat tgcgaagtac
      961 ttctccgcac gggttccgcg ctacacgagt tatcccacgg ccccgcattt ctcggcggac
     1021 gtggatgcca agcgctatga gacctggctg tcccggctcg atcccagctc ggaaatctcg
     1081 ctttatctgc atgtcccatt ctgccgccaa atgtgctggt attgtgggtg caacatgcgg
     1141 gtaatcgccc gatattcccc ggttacgacc tatgtcgata ccatcatccg ggaaatcgaa
     1201 ctcgcggctc gcgtcatgcc tcaaggcatc tccgtgcgtc acattcactg gggaggcggg
     1261 acgccgacgg cgcttgctcc ggaggatatg atccgcatcg cggaaaccgt tcagcgtcat
     1321 ttcaacattc tgggcaccgc agaaatcgcg gtcgagatcg atccgagaac tttcacccgc
     1381 gaaaacgccg ttgcgctcgg caagatcgga tgcacacgcg cgagcctcgg cgtgcaggaa
     1441 ttcgacgaga aggtgcagac ggcgatcaat cgcgttcagc cgatcagtat gatcgaaagc
     1501 acattgacgc tgttgcgcga gcagggcatc accgaagtca acttcgattt gatgtatggc
     1561 ctcccatatc agacggccga aacactcaaa gataccgtgg agcaggccgc gaagcttaat
     1621 ccggctcgca tcgctttgtt cggctacgcg catgttccat ggatggcgaa gaaccagaaa
     1681 atgatccctg aggagtccct gccgacgcca acgatgcgct ttgagcaggc ccaagtcgca
     1741 ggcgatgctc ttgccgagca gggatatgtg aagatcggcc tcgatcactt cgcgaggcca
     1801 gaagacagta tggcggttgc actgcgcgaa ggctgcctgc atcgcaactt tcagggctat
     1861 accaatgacg aggccggcac catcgtcgca ttcggagcga gtgcgatctc ggccctgccg
     1921 gagggcttcg cgcagaacat cgtagagacg ggggcttata cccgcgccgt gaattccgga
     1981 gtgcttccga tcgccaaagg cattgttttc cgcggtgaag acaagatgcg cggcgcgatc
     2041 atcgagcgcc tgatgtgcga gatggaggta gacctcgagg acatccgcaa gcaatatgcg
     2101 ccggaccgga gcggtttctc ggatgaaatg tcacagcttc gcgggcttga ggaagaaggg
     2161 ctctgcacgc tggaaggaag ccgcgtgcgt gtgcccgatg aggcgcgtct cgctctgcgt
     2221 gtcgtgtgct cggtgttcga cgcttatctg gcccagagca cggaacttcg gcacgcagcc
     2281 gcagtctagc gctttagaac cgatcaactt ttccgccttt catcgattcc gatcaaaggc
     2341 ggttgattta agctgtcaac actccagcta agacagggag tcattcgtac tcgccttctc
     2401 acttgttttt ttcagcggct tgcattcgag gcccatccat ggcgcggatt tttatcttcc
     2461 tgctgttcgg aatcgctttt tgctacggcg ctcgcgctga agagggcagc gcagaagcag
     2521 gcttgacgta cgcacgaacc atctgctcgg aatgtcacgc tattcgcagc gacgatgttt
     2581 tatcgcccaa cccaaaagcg ccccccttcg ccaaaatagc cagcacgtcc gggatgaccg
     2641 gggccgcact ctttgtgatt ttgcaaacgc cccacaggga tatgccggac ctcattatcc
     2701 cggcccagga taaggcggat gtcgtggctt atattctgag ccttcaaaaa tagcacaacc
     2761 acttaaagcg ccctccagga cagatcgaaa ccctttaatt ctgataggtt aggctctatt
     2821 tcgggcgttc tcacggctgc gacacaaagg ttgcctttcg ccgtcgaaca gcagcatgga
     2881 cagaaaccga ttatcccccc tccttttgct gcccaacctg ctcagcatgt tccgcctcgc
     2941 ggcggcgccc gtgctggcct gggccgtgct tgcgtctcaa acgcctgctc tcgcgatcgg
     3001 aattgtcatc gccgcagcaa tgaccgatct cctcgacggc cactgcgcgc gcgccactgg
     3061 acaagtctcc gaggtcggcg cagtcctcga cccgattgcc gataagatct tcatcctgac
     3121 cgcactttcg ctgctgtggg gcgagagcat catccgtggc gccaatgcct gggccgtctt
     3181 gatcatcttg tggcgggagg ttctgatctc tggccttcgc gattacgccc gtttcgaggg
     3241 ccatcaggct cccgtaagcc gagtggcaaa gttcaagacg gc
//
LOCUS       DATMGX010000164         3140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_247047, whole genome shotgun sequence.
ACCESSION   DATMGX010000164 DATMGX010000000
VERSION     DATMGX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247047"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="VNR65_02670"
     CDS             complement(<1..1498)
                     /locus_tag="VNR65_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17609.1"
                     /translation="MHLQFSLAVLETPVAVVVLCLGFGLVAYFGFGNFSRMMFALSRG
                     RSQLFSVGAGIMLLVVVFQMHDWSRTKPADTTGEDFMAASAPFAASGAGNFDRAALAT
                     VSTYHNDNARTGQYINETILTPANVNPARFGKLYSYPLDGYVYAQPLYMPQVAIPGNG
                     VHNVVVVATQHDSVYAFDADSSSATPLWRVNFLNPDVGITTLTPADVNTSDIVPEIGI
                     TSTPVIDVTSNTIYVVAASKENGAFYHRIHALDLASGAEKFGGPRAIQATYPGAARES
                     SDGVLAFDSRFHLQRAALLLDKGRLYVAFASYGDFGAYHGWVITYDATTLKQTGSWVT
                     TPNGYQGGIWMSGCGISTDADGNLYLSVANGPFDAFGEQPGVDLSDSVVKLKPGPAGL
                     ALLDYFTPFNQAKMARDDLDLGSAGVVLLPDQSGPYPHLAVTSGKNGHIYLLNRDVLG
                     GYRAEGDRNPQVVQEISGLREQMGTPAYWNGYIYFGSGVSPFKDSIRAF"
     gene            1873..2199
                     /locus_tag="VNR65_02675"
     CDS             1873..2199
                     /locus_tag="VNR65_02675"
                     /inference="COORDINATES: protein motif:HMM:NF028518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWJ17610.1"
                     /translation="MAFRGSNNDANSRKGKSLPPDDTHQEAAYLKMLGEKQCPVRIKL
                     MDGDIVRGWIEYYDRRMVRLTREGAPNLFIFKHEIAYIAEDTGRKARPDGPAPAKNGN
                     SASGSE"
     gene            2196..3029
                     /locus_tag="VNR65_02680"
     CDS             2196..3029
                     /locus_tag="VNR65_02680"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWJ17611.1"
                     /translation="MTHYLPEVEAIAREAGALLMGYFAGHLTIEYKGDVDLVTEADRA
                     SEKLIVERLRARWPEHGIVGEEGTRSGPGADYRWYVDPLDGTTNFAHGYPVFCISIGL
                     VRKDEQIEAGVLYDPTRDEMFSAERGLGATLNGKPIHVSKTTKLAESILGTGFPSHKR
                     HKNPNIHFYHQITLRSHGVRRAGSAALDLANVASGRYDGFWEFNLNPWDTAAGVLLVQ
                     EAGGRVTRFDGSPFRLDSREVLASNGPIHQELLTNFEEIFAGRGLEELPSPVEYAKSR
                     K"
BASE COUNT          677 a          927 c          910 g          626 t
ORIGIN      
        1 tgaaggctcg tatcgaatct ttgaacggac tgactcccga tccaaaatag atgtagccat
       61 tccaatatgc gggcgtaccc atctgctctc gcagcccaga gatttcctgc accacctgcg
      121 gattgcgatc tccctccgcc cggtagccgc cgagaacgtc gcgattgaga aggtagatgt
      181 ggccgttctt gccgctggtg accgccaggt gcggataggg accggactgg tcaggcaaca
      241 gaacgacgcc tgccgatccc aggtccaaat catcgcgcgc catttttgcc tgattgaaag
      301 gagtgaagta atccagcagc gctaacccgg ccggccctgg tttcaacttc acgacgctgt
      361 cactcaagtc cacaccgggc tgttcaccaa aggcgtcgaa ggggccattc gcgaccgaga
      421 gatagagatt gccgtcagca tccgtggaga ttccacagcc gctcatccag atgccccctt
      481 ggtaaccgtt gggggtggtg acccaacttc cggtttgctt cagcgtcgtc gcgtcgtacg
      541 taatgaccca gccgtgataa gcgccaaaat cgccgtagga tgcaaacgca acatacaacc
      601 ggcctttgtc gagcagcagt gcggcccttt gcaggtggaa gcgggagtcg aaagcaagga
      661 caccgtctga gctttctcgc gcggcgccgg gatacgtcgc ctggatagcc cgcggcccac
      721 cgaatttttc cgcacccgaa gccagatcca gagcatggat gcggtgataa aaagcgccgt
      781 tctccttgct ggccgccact acatagattg tgttgctggt cacgtcgatg accggagtgc
      841 tggtgattcc tatctccggc acgatatcgc tcgtgttcac gtccgcgggt gtcaaggtgg
      901 ttatgccaac atcggggttg agaaagttga ctcgccacag cggcgtggca gaactggagt
      961 cggcatcgaa cgcatagacg ctgtcgtgct gggtcgccac aaccacgacg ttgtgaacgc
     1021 cgtttccggg aattgcgacc tgcggcatgt agagcggctg cgcgtaaaca taaccatcca
     1081 gcggatagga atagagcttg ccaaagcgcg cgggattcac gtttgcgggc gtcagtatcg
     1141 tttcgttgat gtactgaccg gtccgggcat tgtcgttgtg gtaggtgctg accgtggcaa
     1201 gagcggcacg gtcaaaattg ccagctccgc ttgccgcgaa tggagcggac gctgccatga
     1261 agtcttcgcc ggtcgtatcc gcgggcttgg tccggctcca gtcgtgcatc tggaacacaa
     1321 ccacaagcag cataatgccc gctcccacgg aaaataactg tgaccgtccc cgcgatagcg
     1381 caaacatcat tctggagaag ttgccgaaac caaagtaggc cacgagaccg aagccgagac
     1441 acagcaccac gaccgcgacc ggagtttcca acactgctaa agaaaattgc agatgcataa
     1501 gattcaagac ttgccagtgg cgcttagctc cctaacacca gaatggaatc aaagtggcga
     1561 cacgcgtaca cattctcaat tcgcggcacg ttctcgataa gatgctcgcg ccgatctcgg
     1621 tgatgcacac tgcaagatgg gcactatggc aggttaatct ggatccttca gccaggcgga
     1681 ataagattgt taagaaatgt gtctctatcg attgatttcc ggtcgcagtg caggggacaa
     1741 tcccgcaatc ggcaatcgtt gtcccagcag ccgaattacg tcgctgaact ctgctaatcc
     1801 accgccgcga accgctatcg caccgttccg attgcaggcg gacagagtcc gtctccgtgt
     1861 agaattcgag ccatggcctt ccgtggatcc aacaacgacg ccaactcgcg caagggcaag
     1921 tctctaccgc ctgacgacac tcaccaggaa gcggcttacc tcaagatgct gggcgagaag
     1981 cagtgtcccg ttcgcatcaa gttgatggac ggcgacattg tccgcggctg gatcgagtat
     2041 tatgaccggc gcatggtgcg gctgacccgc gagggagcgc ccaacctgtt catcttcaag
     2101 cacgagattg cctacatcgc ggaggacact ggacgcaagg cgcgtccgga cggccctgcc
     2161 ccggccaaga acggcaactc tgcaagtggt tcggaatgac gcactacctg ccagaggtgg
     2221 aagcgatcgc ccgagaggct ggggcgctcc tcatgggcta cttcgccggc cacctcacca
     2281 tagagtacaa aggcgacgtt gatttagtga ccgaggccga ccgcgcctcg gaaaagctga
     2341 tcgtcgagcg cttgcgcgcg cgctggccgg aacacggaat tgtcggcgaa gagggcaccc
     2401 gcagcggtcc cggcgccgac tatcgctggt atgtcgatcc gctcgacggc accaccaatt
     2461 ttgcccacgg ctatcctgtg ttctgcattt cgatcgggct agtgcgcaag gatgagcaga
     2521 tcgaggctgg cgtactctat gacccgacac gcgacgagat gttctcggcg gaacgcggac
     2581 tgggtgccac gctgaacggt aagccaatcc acgtttccaa aacgacgaag ctggcggaat
     2641 cgatcctcgg caccggcttt cccagccaca agcggcacaa gaatcccaac atccactttt
     2701 atcaccagat cacgttgcgc tcgcatggcg tgcggcgcgc tgggtcggcg gcgctcgact
     2761 tggccaacgt cgcaagcggg cgctatgacg gcttctggga attcaacctg aatccgtggg
     2821 acaccgcggc gggcgtactt ttggttcagg aagccggcgg acgcgtgacc cgctttgacg
     2881 gttcgccgtt tcgcctcgac agtcgagagg tcctggccag caacgggccc atccaccagg
     2941 agctgcttac gaacttcgaa gaaatcttcg ccggtcgcgg actcgaggaa ttgccctctc
     3001 cggtggagta cgcaaaatcg cggaaatagc cgcgtcttgc ggctcgttac ggtgtttaat
     3061 accccgatga ggaggtatct ctccatgcat cgtttcatga ctcggggatt gcttctctcg
     3121 ctttgcttgc tttctctctg
//
LOCUS       DATMGX010000165         3948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_14930, whole genome shotgun sequence.
ACCESSION   DATMGX010000165 DATMGX010000000
VERSION     DATMGX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14930"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            429..2369
                     /locus_tag="VNR65_02685"
     CDS             429..2369
                     /locus_tag="VNR65_02685"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HWJ17612.1"
                     /translation="MTRIRPAAVSGGGLGIALILCVALPATSMAQDKPVKSDDIGLIN
                     PEAAAKAFPKRGFSPYAGRNYPTRVFWGDEHVHTGWSVDAGAFGATLGPEEALRFARG
                     EQVMSSLGEPAKLSRPLDWAAITDHSDAAGTIFEIRDGNPNLMTDPLLKKWHDMMASG
                     KGVEAASEMINAQSNNRIPAAIKDPRLALSVWKKNTSIMEKYNEPGRFTAFIGYEWTS
                     NAGGGDNLHRNVIYRDGKDKADQVLPMTTFDSENPEDLWKWMASWEAKTGGSLLAIPH
                     NGNLSNGKMFALQTFAGNPLTREWAETRAKWEPMYEATQIKGDGESHPSLSPSDEFAN
                     FEKWDQANLAGVPKKPGMLEREYARQALKEGLKLEQSLGVNPFKFGLAGGTDSHTGLT
                     TAEEDNFFGKHTGVEPSPHRWEHSVIKTPEVNVVGWQMAAAGYTGVWASENTREAIWD
                     ALKRKEAYSTTGPRMIVRFFGGWDFDAKDAQTRMPAEAGYSKGVPMGGDLRQAPAGKS
                     PTFIAAALKDPISGNLDRIQIIKGWVDAKGGLQEKVYDVVWSGDRKAGSDGKLPPVGN
                     TVDVENAIWTNTIGAPELIAAWKDPDFDPKLRAFYYARVIEIPTPRWTAYDQKRFGVK
                     MDAKVPMVVTERAYTSPIWYTP"
     gene            2561..>3948
                     /locus_tag="VNR65_02690"
     CDS             2561..>3948
                     /locus_tag="VNR65_02690"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HWJ17613.1"
                     /translation="MFGDTHLHTAVSVDAGTLNRVGQEDAFRFARGEEVTATGGLRAK
                     LSRPLDFLVIADHAEMYGLMPQLLSGDPEVLATATGKKWYQELKSGNTDRIFATAMDI
                     VGSLSGDVPPIKSDKAVRNAWHAYTALADKYNEPGRFTALIGYEWTAIGGYNLHRNVI
                     FRGNASVANRTIPYSQYDSKNPEDLWNHLAAFEQQTGAEVLAIPHNGNLSNGRMFTVE
                     TFGGKPLTKEIATLRARFEKLVEVTQIKGDGEAHPMLSPNDEFAGYEIWDKSNLNGTE
                     AKKPEMIQWEYAREALKTGLMLGKKLGVNPYKFGMIGSTDSHTSLSTAEEDNFFGKHS
                     GVEPSAHRWEHVVIEAPDPKFTIKGWQQAASGYAAVWATENTREAIFDAMQRKETYAT
                     TGPRMTVRFFGGWDFNKDDTLSRLPAGVGYTKGVPMGGDLPAAPGKKAPSFLVAAMKD
                     PYSGNLDRIQVV"
BASE COUNT          885 a         1191 c         1157 g          715 t
ORIGIN      
        1 cccttcgaag aattacccgg cactcaaatt acctaccgcg gctaaattcc catagcgccc
       61 gttgcaccac cggtgcccaa cttcccgcga cttcgtgcct tggcgctttt cggccgccgg
      121 ccgcctcagc gcgtggatag acctgcatcc cggcagccga aaacctccac attctcggac
      181 attgacccaa agccccgtga gccgtttgca acctattttc actcgttggc cggtcgaaat
      241 gtcagacggc ggccataaag ttgatggttg acccatgtca ttgccgataa ggtttcgccg
      301 tcgcatcgtt gccaattaag tcctaggagg gcgttcggga tcaagtcttc tcagcatcgc
      361 ctcggcccat cagactaaac gtcagggagc agacaatgag ccagtggacc cgttcaggag
      421 caaccggaat gacaagaata cgccctgccg cagtttcagg cggcggcctt ggaattgcac
      481 tgatcctgtg tgttgctctg cctgctacct cgatggcgca ggacaagccg gtcaagtcgg
      541 acgacatagg gctcatcaac ccggaggctg ccgctaaggc ctttccaaag cgcggtttct
      601 ccccctatgc cggccgcaac tatccgacgc gcgtgttctg gggcgacgag catgtgcaca
      661 ccggctggtc agtcgatgcg ggcgctttcg gcgcaacgct tgggccggag gaggcgctgc
      721 ggttcgcgag gggcgagcaa gtcatgtcct cgctcggcga gccggcgaag ctgagtcggc
      781 cgctcgactg ggccgcaatc accgaccatt ccgacgccgc cggtaccatc tttgaaatcc
      841 gggacggcaa ccccaacctc atgaccgatc cgctgctgaa gaaatggcat gacatgatgg
      901 cgtcaggcaa aggcgtcgag gccgcctccg aaatgatcaa cgcccagtcc aacaaccgga
      961 taccggctgc catcaaggat cccaggctcg ctctgtccgt ctggaagaaa aacacttcca
     1021 tcatggagaa atacaacgag ccgggccgct tcaccgcttt catcggctac gagtggacca
     1081 gcaacgcagg tggcggcgac aatctacatc gcaacgtgat ctaccgcgac ggcaaggaca
     1141 aggccgacca ggtcctgccg atgaccacct tcgacagcga aaatccagag gacctatgga
     1201 aatggatggc ctcctgggag gcgaagacgg gcggctcgct tctagcgatc ccacacaacg
     1261 gcaacctctc gaacggcaaa atgttcgcgc tccagacctt cgccggcaat ccgctgacgc
     1321 gcgaatgggc cgagacgcgc gcgaagtggg aaccgatgta cgaggccacc cagatcaagg
     1381 gcgatggtga gtcacatccg tcgctctcgc ccagcgatga gttcgccaat ttcgaaaagt
     1441 gggatcaggc taacctggcc ggcgtgccga agaagcccgg catgctggaa cgggaatacg
     1501 caaggcaggc cctcaaagaa ggtctgaagc tcgaacagtc gctcggcgtg aacccgttca
     1561 agttcggcct cgcgggcggc accgacagcc acacaggtct cacgaccgcg gaggaggata
     1621 acttcttcgg caagcatacg ggcgtggagc cgtcgccgca tcggtgggaa cattccgtca
     1681 tcaaaacgcc tgaggtcaac gtcgtggggt ggcaaatggc cgccgcgggc tatactggcg
     1741 tctgggcgag cgagaacacg cgcgaggcga tatgggacgc cctcaagcgt aaggaggcct
     1801 attcgacaac cggtcctcga atgatagttc gcttcttcgg cggctgggat ttcgacgcca
     1861 aggatgctca gacgcggatg cccgccgagg caggctattc gaagggcgtt ccgatgggcg
     1921 gcgatctccg gcaggcccct gccgggaagt caccgacttt cattgcagcc gccctcaagg
     1981 acccgatcag cggcaacctg gatcgcatcc agatcatcaa gggctgggtg gacgcaaaag
     2041 gtggtctgca ggagaaggtc tacgacgtgg tctggtcggg cgaccgcaag gcgggctccg
     2101 atggcaaact gccgccggtt ggaaacaccg tggacgttga gaacgccatc tggacgaaca
     2161 caatcggcgc gcccgaactg atcgccgcct ggaaggatcc ggacttcgat ccgaaactgc
     2221 gagccttcta ctatgcccgc gtaatcgaga ttccgacgcc gcgctggacg gcctatgacc
     2281 agaagaggtt cggggttaag atggatgcga aagtcccgat ggtcgtgacc gaacgcgcct
     2341 acacctcccc gatctggtac acaccctgac gctgagcacg gagcatcacg ctgtgaaacg
     2401 aacaattcag acgattctca ttcttgccgg ttgcgctgtg gcctcggtcg cctggtctgc
     2461 ggagaccggc aacgatgagg ccatcacgaa ggaggacgtt gcgcaaacgt tcaagcccga
     2521 gttctcgccc tatgccgggc gaaactatcc gactcgtgtc ttgttcggtg acacccactt
     2581 gcataccgcg gtatctgttg atgccggcac gttaaaccga gtggggcagg aagatgcctt
     2641 tcgtttcgcc cgcggcgagg aagtcaccgc cactggcggc ctgcgtgcga agctttctcg
     2701 gccactcgat ttcctggtca tcgccgacca cgccgagatg tatggcctga tgccgcagtt
     2761 gctcagcggc gacccggaag tcctcgccac cgcgacaggc aagaaatggt atcaggagtt
     2821 aaagagcggg aacaccgacc gcatattcgc taccgcgatg gatattgttg ggtctctttc
     2881 tggtgacgta ccaccaatca agagcgataa ggcagtccgc aatgcctggc acgcctacac
     2941 cgcgctggcc gacaaatata acgagccggg acgcttcacg gccctcatcg gctatgagtg
     3001 gactgcgatt ggcggttaca atctccaccg caacgtaatc tttcgcggaa atgcgagtgt
     3061 ggccaaccgc accatcccgt actctcaata cgacagcaag aatcccgaag atctctggaa
     3121 tcatctcgcc gcatttgagc aacaaactgg cgccgaggtt ttggccattc cccacaatgg
     3181 caatctgagc aacggccgca tgttcactgt cgagacgttt ggcggaaaac cgcttaccaa
     3241 agaaattgcg acgctgcgcg cacgatttga gaagctcgtg gaggtcactc agatcaaggg
     3301 cgacggcgaa gctcacccaa tgctctcgcc caatgacgag ttcgccggtt acgaaatatg
     3361 ggacaagtcc aatcttaacg gcaccgaggc caaaaagccc gagatgattc agtgggagta
     3421 tgcccgcgag gcgctcaaga ccggcctgat gttgggcaaa aagctcggcg tgaatcccta
     3481 caagttcggc atgattggaa gtactgattc acacacttcc ttgagcacgg ccgaggagga
     3541 caacttcttt ggaaaacact ccggcgtcga gccgtcggca caccggtggg aacacgtcgt
     3601 aatcgaagcg cccgacccga agttcaccat caagggctgg caacaagctg ccagtggtta
     3661 cgccgccgtt tgggcaacag aaaacacgcg tgaggcaatc tttgacgcta tgcagcgcaa
     3721 ggaaacttat gccaccacag gcccccgtat gaccgtacgt ttcttcggcg gttgggattt
     3781 caacaaggat gacacgctgt cacgtcttcc ggccggcgtg ggctacacca agggcgtgcc
     3841 aatgggtggc gatctcccgg cagcacccgg caaaaaggct cccagttttc tggttgcagc
     3901 gatgaaggac ccgtacagcg gcaatcttga tcggatccag gttgtgaa
//
LOCUS       DATMGX010000166         4732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_215658, whole genome shotgun sequence.
ACCESSION   DATMGX010000166 DATMGX010000000
VERSION     DATMGX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215658"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VNR65_02695"
     CDS             <1..658
                     /locus_tag="VNR65_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ17614.1"
                     /translation="AEAYYRRGTVYRKQNNPSRALEDFTTAIEIDPKQSGAYFARAEI
                     NREKGDLARALADYDAAIALNSRATWAYLSRGAVYQAKGDSAHAVADYGKAIEINPRG
                     ADAYFARANILRGAGSFEKAIADYTHVIEIRPKDADAYNNRAWTYYSLGDPVRGLEDA
                     GKALQIRPYDANALDTLGHILEILGRHEEAIIEYKKAIRLDPDIESSKEGLKRLGANH
                     "
     gene            734..2275
                     /locus_tag="VNR65_02700"
     CDS             734..2275
                     /locus_tag="VNR65_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ17615.1"
                     /translation="MNTKPVWLLAGALLISAPLAAAAASREDVRTLASNVGRVLGAAS
                     ACPSIANARIKGISEKITSAIKSSVASEDDGRAVLGLFNASYLAGARDVNTRQTDCAT
                     ADRELTDLESASVPASQVTAAAPASQLATFATQVAAGSVHGVTDSEIKFGASVPLTGP
                     NKDYGHQIKVGIETAFRMANDAGGINGKMLRLIIADDGYEPTRTAETMKLLYDKEQVF
                     GFVGNFGTATSMVAAPFALDRQSLFYAAYSGANVLRREPPDRYVFNYRASYAEETEAA
                     VRYLVKLRRIKPEQIAVFAQQDAFGDAGFAGVAKAMRALRGGDGGLILRTGYARNSLD
                     VDTAIAQLKASRTPIKAVVMVATYRAAAKFIEKTHEGFPDLIYTNPSAVGSSSLREEL
                     MLLGPQYARGVIVTQVVPAVDGYSSLILEYKKSLAKYFGGEAPDYVSFEYYIASRIMI
                     EALKRAGPQLDSEKLVDAFENMKDFDLGLGTAINFSKSEHQGLHKVWGTQLNEAGTYE
                     PVELQ"
     gene            2373..3923
                     /locus_tag="VNR65_02705"
     CDS             2373..3923
                     /locus_tag="VNR65_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ17616.1"
                     /translation="MQSRSIVLLTSAFLLAGSLSAASAPRDDKGSALRGLVSRVGHLL
                     GAASACPNVAPLRIERISDKITDVIKSSAIGEDESKAIMDLFNTNYSEGGRSVNTKQA
                     DCAAADRELADLESASAPAPQMNAMTAAQPATFATQVSIGPVHGVSDNEIRFGISAPF
                     SGPAKDLGHQMRVGIETAFRMTNETGGVYGRVLKLVTADDGYEPGRTSATMKQLFDQD
                     QVFGFIGNVGTPTAVVAAPFALERRALFYGAFTGANVLRNDPPDRYVFNYRASYAEET
                     DAMVHYLVKVRRLKPEQIAVFAQQDSYGDAGFAGVTKAMRVLRGGDGGFVLRVGYPRN
                     TVEVDTAIGQLKANKTPIKAVVMVATYRAAAKFIEKSKDTIPGLVYSNVSFVGSTSLR
                     DELMLLGPRYAQGVIVTQVVPAVDGYSSLILEYKNALARYFGGEAPDYVSLEGYVAAK
                     VLLEALKRAGPQVDTERVVDALEAMRDYDMGLGTTITFSRSEHQGSHKVWGTQLTEAG
                     KYEPFELQ"
BASE COUNT          996 a         1326 c         1413 g          997 t
ORIGIN      
        1 cgcggaagcc tattacaggc ggggtacggt ctatcgcaaa cagaacaatc caagccgcgc
       61 cctcgaagac ttcacgacgg cgatcgagat cgatcccaag caatctggcg cgtatttcgc
      121 gcgcgccgaa atcaaccgtg aaaagggcga tcttgcccgt gcgcttgccg actacgacgc
      181 agccatcgca ctcaattcgc gcgcgacgtg ggcttacctt tcgcgaggcg cagtctatca
      241 ggccaagggc gattccgcgc acgctgtcgc tgattacggc aaggccatcg agatcaatcc
      301 gcgcggggcg gatgcctatt tcgcccgggc aaacatcttg cgtggcgcgg gttctttcga
      361 aaaggcgatc gccgattata cgcatgtgat cgagatccgc cctaaggacg cggatgccta
      421 caacaatcgt gcctggactt attacagcct gggggatccc gttcggggcc tggaggatgc
      481 agggaaagcg cttcagatcc ggccctatga cgccaatgca ctcgacacgc taggccatat
      541 tcttgagatc ctcggccggc acgaggaagc aattattgag tataaaaagg caatacgcct
      601 tgatccagac attgaaagta gtaaggaagg cttaaagcgg ctcggagcga atcactaatt
      661 cgctccaaag aacaagaccg cgaatgggcg attttccgtt gacggaaaat ctttaagcac
      721 ctgggagcgg aacgtgaata ctaaacctgt ttggttactg gcaggcgcgc ttttgatttc
      781 tgcgcctcta gccgccgccg cggcatcacg cgaggatgtt cgcacgctgg ccagtaatgt
      841 tggacgtgtg ctgggggcgg cctctgcctg tccatccatc gcgaacgccc gcataaaagg
      901 gatttcggag aagatcacga gcgcgatcaa gtcctccgtt gcaagcgaag acgacggcag
      961 ggcggtcctc ggtttgttca acgccagcta tcttgcgggc gctcgcgatg tgaacacgag
     1021 gcagaccgac tgcgcgacgg cagatcgaga gcttaccgat ctcgagagcg cttccgtacc
     1081 agcctcgcaa gtgactgccg ctgcgccggc atcgcagctc gcaaccttcg ccacgcaggt
     1141 cgcggcaggc tcggtccatg gagtcaccga cagcgagatc aaatttgggg catccgtgcc
     1201 gctgaccggt cccaacaagg attacggcca tcaaatcaag gttggtatcg aaacggcgtt
     1261 tcgcatggcg aacgatgcgg gcggcatcaa cgggaagatg ctccgcctca tcatcgccga
     1321 tgacggttat gagccgaccc ggaccgccga aaccatgaag ctgctctatg ataaagagca
     1381 ggtttttggc ttcgttggaa actttggcac agccacgtcg atggtcgccg cgccattcgc
     1441 gcttgatcgt caatccctct tttacgctgc ttatagcggc gcaaatgttc ttcgccgcga
     1501 gccgccggac cgttatgtct tcaattaccg cgcgagctat gcggaggaga ccgaagccgc
     1561 cgtgcgatat ctggtgaagc tgcgccggat caaaccagag cagatcgccg tattcgctca
     1621 gcaggatgct ttcggcgatg caggctttgc gggcgtagcg aaggctatgc gcgcgctaag
     1681 gggcggcgac ggcgggttga tcctgcggac ggggtacgcc cggaattccc tcgacgtcga
     1741 tacggccatc gcccagctga aagccagcag gacgccgatc aaggcggttg tcatggtggc
     1801 gacctatcgc gctgcggcga aattcatcga gaaaacgcac gaagggttcc cggacctgat
     1861 ttataccaat ccgtctgcag tcggcagcag ctccttgcgc gaggagttga tgctgctcgg
     1921 gcctcaatat gctcgtgggg tcatcgtcac gcaagttgtc ccggccgtgg atggttattc
     1981 atctctgatc ctggagtaca aaaagtcgct ggcgaaatac ttcggcggcg aggccccgga
     2041 ctatgtgtcg ttcgaatatt atatcgcctc tcgtatcatg atcgaggcgc ttaaacgcgc
     2101 cggaccacag cttgattctg agaagcttgt ggatgcgttc gagaatatga aggacttcga
     2161 cctgggactg ggaacagcga taaacttcag caaaagcgag catcaggggt tgcacaaggt
     2221 ctggggcacg cagctgaacg aagctgggac ctacgagccc gtggagctgc aatgacggct
     2281 ccatagctgt ggagcctttc ttcgcggcga ttgcgtgcaa tccgagaata cgcaacaagg
     2341 aggtgggctt aaagcaaaga gggtgggttt gcatgcaatc ccgatctatt gtgctcctga
     2401 caagtgcgtt tctgctggcg ggttccctgt ccgcggcatc tgctcctcgc gacgacaaag
     2461 gaagcgcgct tcgcggtttg gtgagccggg ttggacattt gctcggtgca gcctcagcgt
     2521 gtccaaacgt agcgccgctt cggatcgagc gcatctcgga caaaatcacc gatgtgatca
     2581 agtcctcagc gattggcgag gacgaaagca aggccatcat ggatttgttc aacacgaact
     2641 attctgaggg cggtcgctcg gtgaacacga agcaagccga ctgcgccgcc gcagatcggg
     2701 agctggctga tctcgagagc gcttccgcgc ctgcgcctca aatgaatgcc atgaccgcgg
     2761 cgcaacccgc gacattcgcg acacaggttt ctatcggtcc cgtgcacggt gtatccgaca
     2821 acgaaatccg ttttggcata tccgctccgt tctcagggcc cgccaaagac ctgggccatc
     2881 agatgcgcgt cggcatcgaa acggcgttcc gtatgaccaa cgaaacgggc ggcgtctacg
     2941 ggcgggtcct gaagctcgtc accgccgatg atggctatga gcccggccgc acgagcgcca
     3001 ctatgaaaca gctctttgac caggatcagg tcttcggttt catcggcaac gtcggaacgc
     3061 cgacggctgt tgtggcggct cccttcgctt tagaacgccg cgccttgttt tacggtgcgt
     3121 tcacgggagc taacgttttg cggaacgatc cgcccgaccg ttatgtcttc aactaccgtg
     3181 cgagctacgc ggaggaaacc gacgcgatgg tgcactatct ggtgaaggtg cggcggctga
     3241 aaccggagca gatcgcagtt ttcgctcagc aggattccta tggcgatgcc ggtttcgcgg
     3301 gcgtgacgaa ggccatgcgc gtgcttcgag gcggagacgg cggcttcgtt ttgcgcgtgg
     3361 ggtatccgcg caatacggtg gaggtcgata cggccatcgg ccagctgaag gcaaacaaga
     3421 cgccgatcaa ggcggtcgtg atggtcgcga cttaccgtgc ggctgcaaaa ttcattgaga
     3481 agtcgaaaga caccattcca ggcctggtct attccaatgt ctccttcgtc ggcagcacgt
     3541 cgctccgcga cgagttgatg cttctcgggc cgagatacgc tcaaggcgtt atcgtgaccc
     3601 aggtcgttcc ggccgtcgac ggctactcat ctttgatcct cgaatacaag aatgccttgg
     3661 ccagatattt cggtggcgag gcgccggatt atgtctcgct cgagggctat gtcgcggcga
     3721 aggtgctgct cgaggcgctg aagcgggccg gcccgcaggt cgataccgaa cgtgtcgtgg
     3781 acgcccttga agcaatgcgt gactacgaca tggggctcgg gacaacaatc accttcagca
     3841 ggagcgagca ccagggatcg cacaaagtct gggggacgca gctcactgaa gcgggcaagt
     3901 acgagccatt cgaactgcaa taagaccccg gcttttgcct tcagaatgcg tcttgacgcc
     3961 actgagcctg gacggggtgg ttaggaaaat cagccctctt gtccgggctt tttcttgcct
     4021 tctaaccagc acgcgtccgc ctaaattttc tcacaaattc tgacttcatt gcgcagagca
     4081 tcataaacat gagggtgttc gttatggacg atagtttcat cggatttttt caaggcatat
     4141 tcagcgagtt acggaggctt cctctcttta atgaccatta tgacaagctc ttcgttattc
     4201 tcggggttct cctgctcctt tatttcgttg ggtcgctggc caggaacgtg ctcttaggtg
     4261 ccctggttgt tttcctcctg tggggttttc gttatcagga catgggtgag tcccggcacg
     4321 tcaatcggta caccggcgct gtctgtgacg taacgaggga atgttggcaa tccgggaaat
     4381 cgtccgactt ctacgatcgc cgttcagatg accgttcaga cgatcgctta tacggccgtt
     4441 caaacgaccg ctcatacgat cgctcaagct accggtaagg ttggaacagc tcggccacgc
     4501 tcattgaaat ccttcgctcc gcaaccctat tgagggcggc tcggcttgtg ccgaaggctg
     4561 gttgcaactg ccacgaaagg actgagcgtg cccgctgaaa cgatcctgct ccaagactgg
     4621 gaaacgccgt ttgggatgcc gccctttgcc gcggtccgcg acgaggactt cctgccggcc
     4681 ttccaggaag caatgcggtt gcatctgggg gaagtggaag cgatcgccaa ta
//
LOCUS       DATMGX010000167         3176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_147460, whole genome shotgun sequence.
ACCESSION   DATMGX010000167 DATMGX010000000
VERSION     DATMGX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147460"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VNR65_02710"
     CDS             complement(<1..409)
                     /locus_tag="VNR65_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17617.1"
                     /translation="MTIVKKPSAFTVDKAALARTAAGGYPNIRLRRPRQAPWSRALVA
                     ETTLSASNLIWPIFVVDGKMVRQPIPSMPGVERLSVDLAVNAAEEAAELGVPAIALFP
                     YTQTNLRDDTASEAFNPENLVCRTVRAIKSRVPQ"
     gene            673..1668
                     /locus_tag="VNR65_02715"
     CDS             673..1668
                     /locus_tag="VNR65_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ17618.1"
                     /translation="MVKIDVLGAGIVGLWQAFMLRRQGHDVTLWDRAGIPGGVRASQL
                     AGAMLAPYCEGEPGHELALKLGLEALPLWREHYPETAATGTLVVAAARDRADFERFAS
                     STHGHERLTAAQLSELEPALQGRFRDGLFFPEEAHVEPGAAMAHLAREIRRMGVVCEP
                     RAYNEKGGDFVVDCRGLGARDDLKTLRGVRGERILIEAKDVSLTRPVRLLHPRIPFYI
                     VPWPGNHFMIGATLIESADGGPVTLRSAMELLACAYALLPELGEARIVGIDAGVRPSF
                     PDNAPKVIVRDRHILVNGLYRHGFLVSPILAQIVADYIESGAKAEGVLFEDHGEW"
     gene            1646..1843
                     /gene="thiS"
                     /locus_tag="VNR65_02720"
     CDS             1646..1843
                     /gene="thiS"
                     /locus_tag="VNR65_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWJ17619.1"
                     /translation="MKITVNGEARITEARSLEELCRELGLSEAKIATARNGDFVPART
                     RAAIALAEGDMIEVLSPRQGG"
     gene            1847..2677
                     /locus_tag="VNR65_02725"
     CDS             1847..2677
                     /locus_tag="VNR65_02725"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HWJ17620.1"
                     /translation="MSSRANGAHETSDVLTLYGETVQSRLLLGTSLYPSPDIMRRSVE
                     ASGAGILTVSLRRESSRDAGAGSRFWDMVKGFGARILPNTAGCHTPREAITTAQMARE
                     VFETNWVKLEVIANDDTLQPDVFGLVEAAEVLCRDGFQVFPYCTEDLSVAERLLRAGC
                     QVLMPWAAPIGTGRGLMNPYALRSLRAYFPDTPLIVDAGIGAPSHAAEAMEMGFDGIL
                     LNTAIAKAHDSVAMAAAFAKAIEAGRLAYRAGIMPPRDMASPSTPVAGLAFLDTARAS
                     "
     gene            2667..>3176
                     /locus_tag="VNR65_02730"
     CDS             2667..>3176
                     /locus_tag="VNR65_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWJ17621.1"
                     /translation="MLPRFYPIVPDAAWAGRLARVGVKLIQLRMKDVPHTAIAPAIRE
                     ALVACRAYGCELVVNDYWQEAIDCGAGFVHLGQGDLQTADTAAIRRAGLKLGLSTHSH
                     EELDRALLEEPDYIALGPIYETTLKVMPWAPQGLARIGEWKALAKQPLVAIGGITLAR
                     APEVFGAGAD"
BASE COUNT          571 a          980 c         1007 g          618 t
ORIGIN      
        1 tctgaggtac gcgcgacttg atggcgcgga ccgtgcggca gacgagattt tccgggttga
       61 acgcctcgct cgccgtgtcg tccctaaggt tcgtctgcgt ataggggaaa agcgcaatcg
      121 cgggaacacc aagctctgca gcctcttcag cggcgttgac cgcgagatcc accgaaagcc
      181 gctccacgcc cggcatggaa ggaatcggct gccgcaccat ttttccgtcc acgacgaaaa
      241 tcggccagat caggttcgag gcagacagag ttgtttctgc gacaagagcg cgcgaccagg
      301 gcgcctgtct tggccgcctc agtctgatat tgggatatcc acctgcggct gttcttgcga
      361 gtgctgcctt atcgacagta aatgccgatg gtttctttac aattgtcatg gcgcggccct
      421 tcctggagag cgttttctgt acgcctgaaa ggcgcttgac acaaggccgg agttaccgta
      481 gaacaaacac agaacatcga tggtcgcccc ccaacaagcc atcgttgccc aaaacaagca
      541 agcgagccag aaaataccgg taaatcagcc ccctaccgga ttatccgttc cttttgcccc
      601 cgaaagcgaa cgaaaacgcc cccaaggctc gcttgcttgc ttttttcctg aatctccgcc
      661 aaatcacgag atatggtaaa aatcgacgtt cttggcgctg gcattgtcgg cctctggcag
      721 gcgtttatgc tgcgcaggca gggccacgat gtcaccttgt gggatcgtgc cgggataccc
      781 ggcggggtgc gggcgagtca gctcgcgggc gcgatgctgg cgccttattg cgaaggcgag
      841 cccggacacg agctggccct gaagctcggc ttggaggccc tgcccctctg gcgggagcat
      901 tatccggaaa cggcagcgac gggcacgctg gttgtggcgg cggcccgcga ccgcgccgac
      961 ttcgagcggt tcgcctcatc gacgcacggt cacgaacggc tgaccgcggc gcagttaagc
     1021 gaactggagc cggcgctgca gggccgcttc cgcgacggac tcttctttcc ggaagaagcg
     1081 catgttgagc ccggcgcggc gatggctcat ctcgcgcgcg aaattcggcg gatgggcgtg
     1141 gtgtgcgaac cgcgcgccta caatgagaag ggcggcgact ttgttgtcga ttgccgcgga
     1201 cttggcgcgc gcgacgacct caagaccttg cgcggtgtgc gcggggagcg catcctcatc
     1261 gaagccaagg acgtatccct gacccggccc gtccggttgc tgcatccgcg catccctttc
     1321 tatatcgtgc cttggcctgg caaccacttc atgatcggcg ccactctgat cgaaagcgcc
     1381 gatggcgggc cggtaacttt gcgctcggcg atggaactct tggcttgcgc ctatgcgctg
     1441 cttcctgagc ttggcgaggc gaggatcgtt ggcatcgacg ccggtgtacg gccaagtttc
     1501 ccggataacg cgccaaaagt gattgtgcgg gaccgccata tcctcgtgaa tggcctttac
     1561 aggcacggat ttcttgtctc gcccatactc gctcagatcg tggccgacta tatagagtcg
     1621 ggcgccaaag cagagggagt tctctttgaa gatcacggtg aatggtgagg cccgcatcac
     1681 cgaggcaagg agccttgaag agttgtgccg ggagcttggg ctttcggaag cgaagattgc
     1741 gacagcgcgg aatggggatt tcgtgccggc cagaacgcgc gctgcgattg cgcttgcgga
     1801 aggcgacatg attgaagttc tttccccgcg ccagggagga tgaagcatga gttcccgcgc
     1861 gaatggtgcg catgagacct cggacgtctt gacgctgtat ggcgagacgg ttcaatcgcg
     1921 gctcttgctt ggcacctctt tatatccctc cccggacatc atgcgacgtt cggtcgaggc
     1981 ttccggcgca ggcatcctga ccgtttctct gcgccgtgaa agtagccgcg acgccggcgc
     2041 gggctcgcgg ttctgggaca tggtgaaagg cttcggagca cgcattctgc caaatacggc
     2101 cgggtgtcac acgccgcggg aagccatcac aacagcgcaa atggcgcgcg aggtcttcga
     2161 gacgaattgg gtgaagctcg aagtcatcgc gaacgacgac acgcttcagc ccgatgtctt
     2221 cggccttgtt gaagcggcgg aagtcttatg ccgtgacggc ttccaggtct tcccttattg
     2281 caccgaggat ttgagcgtgg ccgagcggtt gctacgcgcc gggtgccagg ttctcatgcc
     2341 atgggcggct cccatcggca cggggcgcgg gctcatgaac ccctacgcgc tgcgcagctt
     2401 gcgcgcgtat ttcccggaca cgccgctcat tgtcgatgcc gggatcggcg cgccctccca
     2461 tgcggccgag gccatggaaa tgggcttcga tggcatcctc ttgaatacgg cgatcgccaa
     2521 ggcgcacgac tcggtggcca tggcggccgc cttcgcgaaa gccatcgaag ctggccgcct
     2581 cgcctatcgc gccggtatca tgccgccccg cgacatggcc agcccctcga cacccgtcgc
     2641 tggcctcgcc ttcctcgata ccgcccgtgc ttcctagatt ttaccccatc gtgccggatg
     2701 cggcctgggc cgggcgcttg gcgcgcgtcg gcgtaaagct catccaactg cggatgaaag
     2761 acgtgccgca caccgcgatt gccccggcga tccgcgaggc gctcgtggcc tgccgcgcct
     2821 atggctgcga gcttgtcgtc aatgattact ggcaggaggc catagactgc ggcgcaggct
     2881 ttgtgcatct cggccagggc gacctgcaga cagccgatac cgcagcgatc cgccgcgcgg
     2941 ggttaaaact cgggctctct acccatagcc acgaagagct tgaccgcgcc ttgctcgaag
     3001 agcccgacta cattgcgctc ggccccatct atgaaacgac gctcaaggtc atgccctggg
     3061 cgccgcaggg ccttgcccgg atcggcgaat ggaaggcgct ggcgaagcag ccgctggtgg
     3121 cgatcggcgg cattaccctt gcgcgtgcgc ctgaggtgtt tggcgcgggc gcggac
//
LOCUS       DATMGX010000168         2060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_14903, whole genome shotgun sequence.
ACCESSION   DATMGX010000168 DATMGX010000000
VERSION     DATMGX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14903"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..548)
                     /locus_tag="VNR65_02735"
     CDS             complement(105..548)
                     /locus_tag="VNR65_02735"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWJ17622.1"
                     /translation="MPNTVRLHRVVATKPEKIYRAFLEGDALAKWLPPNGFTCTVHEL
                     DAKAGGRFKMSFRNFTTGQSQSFGGEYLELLPYERIIYTDKFDDPNLPGEIQVTVTLK
                     QVSCGTELHIVQAGLPDVIPPEACYLGWQQSLRNLARLVEPEINQ"
     gene            complement(603..965)
                     /locus_tag="VNR65_02740"
     CDS             complement(603..965)
                     /locus_tag="VNR65_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17623.1"
                     /translation="MTTNTYLAVFLGSKSSPRMAAWIALPEEERQAKTKEGMAAWKAW
                     ADRHQASIVEMGGPLGKTKKISSHGVEDVSNDMGAFMLVRAASQEEAAKLFEHHPHFS
                     IFPGQAVEVMPVLPIPQG"
     gene            complement(962..1579)
                     /locus_tag="VNR65_02745"
     CDS             complement(962..1579)
                     /locus_tag="VNR65_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ17624.1"
                     /translation="MLKIKSAKTKILEAALSVIRAKGYTATTVDDLCAAAGVTKGAFF
                     HHFKSKEELGVAAANYWSEMTGAFFAAAPYHQHADALERVLGYIDFRKSILVGSVPEF
                     TCLAGTMVQELYETSPAIRDACDAAISDHAEKVAVDIAEAMRERCMRADWTAQSLALH
                     TQAVLQGAFILAKAKGGAGIAAASVDHLRRYIELLFSTQPKRRTS"
     gene            1863..>2060
                     /locus_tag="VNR65_02750"
     CDS             1863..>2060
                     /locus_tag="VNR65_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ17625.1"
                     /translation="MPQSSVWLITGCSTGFGRELVRVLAARGHRVVATARNPATLEEF
                     ASNPQVLTAALDVTDSDQIGRA"
BASE COUNT          404 a          602 c          591 g          463 t
ORIGIN      
        1 gcaaaggcgg ttgtccgata gatagccgag gcgacgatgc gagcttgaat gaccccttga
       61 acaggatcac cgctcaaggg gccacgcttg cgtctgcgtt cgagctattg attgatctca
      121 ggctctacaa gtcttgccag atttcgtaag gactgctgcc agccgagata acaggcctcc
      181 ggcgggatga cgtccggcaa cccagcctgc acaatatgca attctgtgcc gcacgacacc
      241 tgcttgagcg tcaccgtcac ctggatttca cccggcaggt tgggatcatc gaatttgtcc
      301 gtgtagatga tacgctcata aggcaagagt tcgagatatt caccgccaaa cgactgactc
      361 tggcctgtgg tgaaattccg gaaagacatt ttaaaccggc ctccggcttt cgcatccagt
      421 tcgtgcacgg tgcaggtgaa accattgggt ggtagccatt tcgcgagcgc atcaccctca
      481 agaaaggcgc gatagatctt ttcgggtttc gtggccacga cccgatgaag acggacggtg
      541 ttgggcatct tttcctcctg agagcctgtt ttcttgccga aattccggcg cgcaaccgca
      601 gcctatccct gcgggatcgg taacaccggc atcacctcca cggcttgtcc aggaaagatc
      661 gaaaagtggg ggtggtgttc gaacagcttg gcggcttcct cctgtgacgc agcgcgaacg
      721 agcatgaaag cgcccatgtc gttgctcacg tcctcaacgc cgtgcgagct tatcttcttc
      781 gttttgccga gcggtccgcc catctcgacg attgaggctt gatgcctatc ggcccacgcc
      841 ttccaggccg ccatgccctc ctttgtcttg gcttgccgct cctcctcggg aagcgcaatc
      901 catgccgcca tgcgcgggct cgatttgctg ccgagaaaaa cagcaagata ggtgttggtc
      961 gtcatgaggt cctccttttg ggctgggtgc taaacagaag ttcgatgtag cggcgcaaat
     1021 gatcaacact cgcggcagcg ataccggcgc cgcctttggc cttggccagg atgaacgcgc
     1081 cttgcagaac ggcttgcgtg tgaagggcga ggctttgcgc cgtccaatct gcgcgcatgc
     1141 agcgctcacg catagcttcg gcgatgtcga ctgcgacttt ttccgcgtga tcgctgatcg
     1201 cggcgtcgca ggcatcgcga atggcggggc tggtctcata aagctcctga accatcgtgc
     1261 cggcaagaca ggtgaattca ggaacgctgc caacgagtat ggacttgcga aaatcgatat
     1321 agccaagcac gcgctccaaa gcatcggcgt gctggtggta gggtgcggcg gcgaaaaacg
     1381 cgcccgtcat ttcggaccag tagttggcgg cggcgacgcc taactcttcc ttgctcttga
     1441 aatggtggaa gaaggcgccc ttcgtaacgc ccgctgcggc gcagagatca tcaaccgtcg
     1501 tggctgtata acccttggcc cgtatcacag acaaagcggc ctccaggatt tttgttttgg
     1561 cgcttttgat tttgagcatt tttgcaccag atgcgtcagg cacgacaaaa ccataccaac
     1621 tggttggtat ttactgcaag taacataggc cgagatcgcg gccttgtgac tgcctgaggc
     1681 gtagcctttg ggtttcgctt tgctgcagcc ttctgtccat ggcgtcttga agagtgatgg
     1741 caggacattc aaattcggca gatgctccgt cccggtcctc accagtccgt cccaggcctt
     1801 cgcacaaagg atcttggata ggttcggcct cgctaccggc cgatcagctt atggagacgc
     1861 ttatgcccca atcttctgtt tggctcatca ccggttgctc gaccggcttt ggccgcgaac
     1921 ttgtccgggt gctcgccgcg cgcggccacc gcgtggtcgc aaccgcgcgc aatcccgcga
     1981 cgcttgaaga gtttgcgtcg aatccgcagg tgctgaccgc cgctctcgac gtcaccgact
     2041 ccgatcagat cggcagggcc
//
LOCUS       DATMGX010000169         2506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_246954, whole genome shotgun sequence.
ACCESSION   DATMGX010000169 DATMGX010000000
VERSION     DATMGX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246954"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..680)
                     /locus_tag="VNR65_02755"
     CDS             complement(309..680)
                     /locus_tag="VNR65_02755"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ17626.1"
                     /translation="MTIYFEIEADDPARAINFYSQVFGWRFSQDQGPERYWRIEAGEF
                     NGGLLPRSAIRPPSQCGANAFVCSFEVETFDAAEQTIQRLGGSVVEPKFPVPGQSWQG
                     YFMDTEGNNFGIYQVDEKAGV"
     gene            1011..2345
                     /gene="gltA"
                     /locus_tag="VNR65_02760"
     CDS             1011..2345
                     /gene="gltA"
                     /locus_tag="VNR65_02760"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWJ17627.1"
                     /translation="MTYDPHTTHKPDAVMPAGSATLTLDNKQYELPVISGTSGLHVID
                     ITKLYGQSGAFTYDPGFASTASCKSDITFTDGEHGKLFYRGYPIEQLAEYSTFLESCH
                     ILLYGDLPNKERYLDFKKRVTSHTMLHEQMHRFFSGYRRDAHPMSVMIGTVGAMSAFF
                     HDSTDITDPAQRELASLRLIGKMPTIAAMAYKYSIGQPFMVPRNDLDYASNFLYMMFG
                     VPTETWKVNPALSRAMDRILILHADHGPNASASTVRLAGSSGANPFACIAAGIACLWG
                     PAHGGANEAALKMLMEIGTVDRIPLYIKRAKDKKDKFRLVGFGHRVYKTYDPRALIMK
                     EICKEVLEITGVKDDPLMQVALELERIAREDDYFIEKKLFPNIDFYSGITLRALGLPT
                     TMFTVLFAMARTAGWIAQWKEMIADPEQKIGRPRQLYIGATPREYVPLELRS"
BASE COUNT          535 a          773 c          694 g          504 t
ORIGIN      
        1 ggtgcgcatg tgaagagctg gccattggcg aggactgata ccgcgctgac tgggcgcgtt
       61 ctctgatgct tcacatagcg ggcaggaacg ccgctgccgc gcgactccct cctgggccat
      121 aagctgacgc gatcaggaag cccaagttct ctttcgggcg cgccagctac gcgcgggact
      181 cctgcccgac cagcaaatcc ccgacgataa gcacaatatc aaagggtcca tgatccataa
      241 gcggcagccg gtaccagcac tacccccgcg atatcacccg ggggggcggg ggcggaagct
      301 gggctgagct agacacctgc cttttcatcg acttggtaaa ttccgaaatt attaccttcg
      361 gtatccatga aatagccctg ccagctctga ccggggacgg ggaatttcgg ttccacaaca
      421 gacccgccaa gcctttgtat tgtctgctcg gccgcatcga aagtctcgac ctcgaacgag
      481 cagacaaagg cattcgcgcc gcactgggag ggggggcgta tggccgaccg aggaagcaaa
      541 cctccattaa attctcccgc ttcgatgcgc caatagcgct cggggccctg gtcttgggag
      601 aacctccagc caaaaacctg agaataaaag ttaatcgctc gcgccggatc gtcagcctcg
      661 atctcgaaat agatggtcat agcactctcc gctttgtgtc gtgtgcgtgc catctcacca
      721 cacctgcgca cctccggcaa ttctttggac cccaccctca cgaagccaat aacgaaggtc
      781 tgaagggtcg catcaagccc cttagagcgt cggccgctca agcgcgaaca tatcctgcgg
      841 ctatgaagtc gttttgctat ttttagggcg agtttgggct gccggtatcg ccaatcgcgc
      901 gccaaaggtc tcggtcattg ctgcaaccgc gaaaatggcc tagaaaatgt gttagagcct
      961 catcacctta cgtgccaagg ttgccccatg cgttctggca ggaggagagc atgacctacg
     1021 atccacacac aacccacaag ccagatgctg tgatgccggc tggttccgcg acgctcacct
     1081 tggacaacaa gcagtatgag cttccggtca tttcaggaac cagcgggctg catgtgatcg
     1141 acatcacgaa gctctacggt cagtccggcg ctttcaccta cgatcccggt tttgcctcga
     1201 cggcgagttg caaatccgac ataaccttta ccgatggcga gcacggtaag ctcttttatc
     1261 ggggctatcc catcgagcag cttgccgagt acagcacctt cctggaaagc tgccacatcc
     1321 tgctttacgg cgatttgccg aacaaggagc gctacctcga tttcaagaag cgcgtgacct
     1381 cccatacgat gctccatgag cagatgcacc gcttcttctc cggttaccgg cgcgacgccc
     1441 acccgatgtc cgtgatgatc ggaaccgttg gcgcgatgtc cgcctttttc cacgactcca
     1501 ccgacatcac cgatccggcc cagcgcgaac ttgcctcgct ccgattgatc ggcaagatgc
     1561 cgacgattgc ggcgatggct tataaatatt ccattggcca acccttcatg gtcccgcgga
     1621 acgacctcga ttacgcttcg aacttccttt acatgatgtt cggggtgccg accgagactt
     1681 ggaaggtcaa tccggcgttg agccgcgcca tggaccgcat cctcatcctt catgccgatc
     1741 acgggccgaa cgcctccgct tcgacggtgc gcctcgccgg atcctccggg gccaatccct
     1801 tcgcctgcat cgcggcgggc atcgcctgcc tgtggggccc cgcgcatggg ggcgccaacg
     1861 aagcagcgct gaagatgctc atggaaatcg gcacggtcga ccgcatcccg ctttacatca
     1921 agcgcgccaa ggacaagaag gataagttcc ggctggtcgg ctttgggcat cgcgtttaca
     1981 agacttacga tccccgcgcg ctcatcatga aggagatttg caaggaagtg ctcgagatta
     2041 ccggtgtcaa ggatgacccg ctcatgcagg tggcgctgga actcgagcgc attgcgcgcg
     2101 aagacgacta tttcatcgag aagaagctgt tccccaacat tgacttctat tcgggcatca
     2161 ccttgagagc gttaggtttg ccaaccacca tgttcacggt gctgttcgcc atggcacgaa
     2221 ctgcgggctg gattgcgcag tggaaagaga tgatcgccga tccggagcaa aaaatcggtc
     2281 ggccgcgcca gctctacatc ggcgcgacgc cccgggagta cgtgcccctg gagttgcgat
     2341 cctaaagcag gatttcgcgg tcgcgccatc ggatcgccgc cgccgtcatc ccggcaagca
     2401 aagcgcgagc ccggatcgca aaaatgcaga cgcttcaatt tgttacgatc ccggataagg
     2461 cttcgccttt ccgggatgac gcagcgcaga gcgagcgtcg tcggga
//
LOCUS       DATMGX010000170         2396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_315653, whole genome shotgun sequence.
ACCESSION   DATMGX010000170 DATMGX010000000
VERSION     DATMGX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315653"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VNR65_02765"
     CDS             complement(<1..689)
                     /locus_tag="VNR65_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HWJ17628.1"
                     /translation="MDKKHQLNLTYIAIAFGLLLLFQFYWVSYNQIESVPYSRFQQLL
                     KDKEVTQVVVGPAQIRGEFKNPQDGKKYFSTTRVDPAVADELGKYGVDFSGSAGQNLL
                     RDILSWVLPVLVFFGIWAFFIRRIADKQGLGGLMSVGRSRAKIYVEKVTGVTFDDVAG
                     ADEAKDELKEVVNFLKDPEPYGRLGARVPKGILLVGPPGIGKTLLARAVAGEAGVPFF
                     SISGSEFVEMFV"
     gene            complement(918..>2396)
                     /locus_tag="VNR65_02770"
     CDS             complement(918..>2396)
                     /locus_tag="VNR65_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWJ17629.1"
                     /translation="DVFQHLTKLSPAFYEKTHSGEIMSRLNADTTQIKAAVSTSVSQV
                     LRNGVMFVGAVAMMMITSLKLSLLVIVVIPLIIVPLILYGRTVRRFSRIAQDEVAASS
                     AFASENLSAMRTMQAFTSEDSVANRYAVAVRRSFDAAVSRTQRRAMLTASAIFLIFGS
                     IVGVLWYGAHDVMAGITTGGVLAQFVLYAVLAAASISEFSEVFGEMQSAAGAAERLSE
                     LMSVRTEIESPRDPRPFPEQAKGEVTFDNVSFTYPLRPDDRALHNVSFQVRPGERVAI
                     VGPSGSGKSTIFNLILRFYDAQGGTVLVDRVPVENADLTALRRRISLVSQDTVIFAES
                     VMDNIRYGSPNATGEQVRKAADAALASTFIEQLPQGYDTLLGERGVVLSGGQRQRIAI
                     ARALLKSSPILLLDEATSALDQESEKLVQTALESVMHGRTTLVIAHRLATVINADRIL
                     VIDGGLLVEEGTHASLINAGGVYSRLAGLQLTGQEGAALVSS"
BASE COUNT          501 a          737 c          724 g          434 t
ORIGIN      
        1 acgaacatct ccacgaactc cgagccggaa atggagaaga acggcacgcc cgcctcgccc
       61 gcgaccgcgc gcgctagcag cgttttgccg atgccgggag ggccgacaag caggatgcct
      121 ttcggcacgc gcgcgcccag acgcccatag ggttcggggt ctttgaggaa attgacgacc
      181 tctttcagct cgtccttcgc ttcgtccgcg cctgccacat cgtcgaacgt gacgcccgtg
      241 accttctcaa cgtaaatttt cgcgcggctg cgccccacgc tcatcaggcc gccaaggccc
      301 tgcttgtcgg caatgcgccg gatgaaaaag gcccaaattc caaagaagac taggacagga
      361 agcacccagg agagaatatc gcgaagcagg ttttggcccg cgctgccgct gaaatcgacg
      421 ccgtatttcc caagctcatc ggcgaccgcg ggatcaacgc gggtcgtgct gaaatatttc
      481 ttgccgtcct gtgggttctt gaactcgcct cttatttgcg ccgggccgac aacaacctgt
      541 gtcacctcct tgtccttcag gagctgctgg aagcggctgt aggggacgga ctcgatctgg
      601 ttgtagctca cccaatagaa ctgaaacagg agcaaaagcc cgaaggcgat cgcaatatag
      661 gtcaggttaa gctgatgttt cttgtccatc gtcgcatccc gttgtgaccg tctccactta
      721 agggcgatcc gcgcccggcg taagggtggg tcttgtaaag ttgattgcat aagtcagcgc
      781 ttcgcgccgc gatgcaacag ccgtctgaga aatgccaacg cacgctggtg gccgtcctaa
      841 acacggccaa tctgcccaaa tgagactgcg aggaaggact gggagcgtta aggggcggac
      901 gaatgcgact gcccccgtca agacgaaacc agcgcggccc cttcctgtcc ggtgagctga
      961 agtcccgcaa gcctggaata gacaccgccc gcattaatga gcgaggcgtg agtgccttcc
     1021 tcgacgagga ggccgccgtc tatcaccaag atgcggtcgg cattgatgac tgtagcaagg
     1081 cggtgggcga taacgagcgt cgtgcggccg tgcatgacgc tctccagcgc cgtctgcacg
     1141 agcttttcgc tctcctggtc gagcgcgctt gttgcttcat cgaggagcag gattggggag
     1201 ctcttcagaa gcgcgcgcgc aatggcgatg cgctgacgct gtccgcctga aagcaccacg
     1261 ccgcgctcgc caagaagcgt gtcgtagcct tgcggaagct gctcgatgaa ggtgctcgcg
     1321 agggcagcat ccgcggcctt cctgacctgc tcacccgtgg cattcggtga cccgtatcgg
     1381 atgttgtcca tcacggactc ggcaaagatc acggtgtcct gtgaaacgag cgagatccgc
     1441 cttctcaacg ccgtgaggtc cgcgttctcc acaggaacgc gatctacgag aacggtgccg
     1501 ccttgagcgt cataaaagcg caggatcagg ttgaagatcg tgctcttccc cgaacccgac
     1561 ggcccgacga tcgccacgcg ctctcctggg cgcacctgga aagaaacgtt gtggagggcg
     1621 cgatcatcgg ggcgcaaggg ataagtgaac gacacgttgt cgaaggtcac ctcgcctttc
     1681 gcctgctccg ggaacggacg cgggtcacgg ggagattcga tttcggtcct gaccgacatc
     1741 agctcggaaa ggcgctcggc tgcgccggcc gcagactgca tctccccgaa aacttcggag
     1801 aattcgctga tcgaggcggc ggcaagcaca gcatagagaa caaactgcgc gagaactccg
     1861 cccgtcgtga ttcccgccat cacgtcatgg gcgccgtacc aaagcacgcc gacgatgctg
     1921 ccgaagatga ggaaaatcgc cgacgccgtc agcattgccc ggcgctgcgt ccgcgagacc
     1981 gcggcgtcga aggagcgccg cacggcaacc gcgtagcggt tcgcaacgct gtcctcggac
     2041 gtgaacgcct gcatcgtgcg catggccgac agattttcgc tcgcaaacgc ggaagatgca
     2101 gcgacctcgt cctgggcgat gcggctgaaa cggcgcaccg tgcgcccgta aagaatcagc
     2161 ggcacgatga tcagtgggat cacaacgatc acaagcagac taagcttcag gctcgtgatc
     2221 atcatcatcg caaccgcgcc gacgaacatg acgccgttgc ggagcacctg gctcacggag
     2281 gtcgagactg ccgccttgat ctgcgtcgta tcggcattga gccgcgacat gatctcgccg
     2341 gagtgcgtct tctcgtagaa cgccgggctg agcttcgtca aatgctggaa cacatc
//
LOCUS       DATMGX010000171         2117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_190910, whole genome shotgun sequence.
ACCESSION   DATMGX010000171 DATMGX010000000
VERSION     DATMGX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190910"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(594..815)
                     /locus_tag="VNR65_02775"
     CDS             complement(594..815)
                     /locus_tag="VNR65_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17630.1"
                     /translation="MGYPGAARWPRQCNRTGGISPLGQTRRTPAAIEEESLAHGLVYI
                     NGGQRGLHVRLKAQGLVSALNAIVVPVVA"
     gene            1263..1667
                     /locus_tag="VNR65_02780"
     CDS             1263..1667
                     /locus_tag="VNR65_02780"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWJ17631.1"
                     /translation="MTKTKSTATAKTAASRKPVAAAKTAAPAKAPAAGKAAAKPAKVE
                     LVTLRQLAGSIGEAHGLSQKQANEMLAGTVALIGAHLKKGARIRIVGLGTLEVRKRAA
                     RMGRNPDTGEAIEIKASKKVAFRAAKDLSDAI"
     gene            complement(1711..>2117)
                     /locus_tag="VNR65_02785"
     CDS             complement(1711..>2117)
                     /locus_tag="VNR65_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ17632.1"
                     /translation="QAGWHASNALAKPANVTLVPLPAYSPELKPVERVWLYLNQRFLS
                     HRLLADYDAIVDAACNAWNRLLADAGRIKSLCSYHSNAHGSAARSLRSLHKSRLSRPE
                     PRRSRDSRPKAKFHQKMAQKARIRDETMTAYS"
BASE COUNT          488 a          576 c          659 g          394 t
ORIGIN      
        1 tacaaattga gacgtcgtta ccgtcgctct gattgatatg atcagaacgg agagcgcaaa
       61 aacattctaa aagagtctct ctgcccggag ttctcacacg tggcgtaatt ccgggtcccc
      121 gccacacgga ggtacggggg aatcagctcc agaaaatgat tgaggccacc gaccaaggcc
      181 ttgcaatgat ggaggcacat gcgatgcagc actatgggat tggacggatg tggatcgacg
      241 gcagcgagtt ctgctggtca tcgaaagaac aaccaatgca gcagtccgtc taatcggaac
      301 aacgagttct cgccgaagga cgagaaaaaa catccctgct gggacgtggc aatggcgaga
      361 tcgtcccagg ctctgcgata tccgaaagga caaagcgcgc ttcacacatt gatccgccaa
      421 cgtcgccagg cgccagcgaa gggcattcta gagccagaac gcggagagaa ccgtgggccc
      481 ggcaaggaag tttacagaga gcttgactcc agacccggaa ttagagaaca gccttggtct
      541 tgacaccccg tttgcagatt gaccggcgat aaaggcgttt aaccgcacca gaattacgcc
      601 acaaccggta ccacgatggc attgagcgcc gagaccaagc cctgtgcttt caaccgaaca
      661 tgcagtccgc gctggccgcc attgatgtag acgaggccgt gcgcgagcga ttcctcttcg
      721 attgccgccg gagttctacg cgtctggccg agggggctga tgccgccggt ccgattgcat
      781 tggcgcggcc accgggcagc gcccggatat cccaacgaag ggcacttcgc gggctgtttc
      841 gagcaggaac gccgtgaacg cgcttgaggt tgccgatccg ggatcgaggg gcttcgcgcc
      901 ccgtccttta tgggccagct ttctgcaact cgaatgcaat gaggccgaac aactcagcct
      961 ccgggcagcg tgaggcgagc gagatccgga tgaatcaatc agcgcataag tgagcccgac
     1021 gatctctttc agcgtcggcg cattctccga gcgcgagacc gtgggctggg atcgttcgct
     1081 tcttgaaggc caaacttacg cgtttgaaat tagcgcaaga atcagcgtag cgcatgaaaa
     1141 agtttgcttg gggtagtgtc gccatgcgaa gccgatctac gtagcggagc taggccttcc
     1201 gtgcggcgct ggcaagttcg tcgagcggga atttcgaaga ctgtcaatga aagaagagat
     1261 caatgacaaa gacaaaatca acggcgaccg caaaaacggc agcctcgcgt aaaccggttg
     1321 cggcagccaa gacagcggct ccggccaaag cgcccgcagc gggcaaggca gccgcaaagc
     1381 cggcgaaggt ggagcttgtc acgcttcgcc agctcgcggg ctcgattggt gaagcgcatg
     1441 ggctttcgca aaagcaggcg aacgagatgc tggccggtac agttgccttg atcggcgcgc
     1501 accttaaaaa gggcgcgcgt atccgcatcg tcgggctcgg aactcttgag gtgcgcaagc
     1561 gcgccgcccg catgggacgc aaccccgaca caggcgaagc gattgagatc aaggcgagca
     1621 agaaagtggc gttccgtgcc gcgaaggacc tcagcgacgc gatttaggct gttgtttttg
     1681 atgcgtgcaa tcgttgcccc gggatgcatc tcaagagtac gcggtcatcg tttcgtcccg
     1741 aattcgagct ttctgggcca tcttttggtg aaacttggct ttggggcgtg aatctcgtga
     1801 tctccgaggc tcaggtcttg aaagccgcga tttatggagt gatcggagcg accgagcagc
     1861 gctgccatga gcgttcgagt gataggagca tagcgacttt atccgcccgg catctgccag
     1921 tagcctgttc caggcgttgc aggcggcgtc aacgatagca tcgtaatcgg cgagcaaccg
     1981 gtgcgagaga aagcgttgat tgagataaag ccagacgcgt tcgaccggct tcagttcggg
     2041 cgaataggct ggcagcggga ccaaagtgac gttggccggc ttggcgaggg cattcgaggc
     2101 atgccagcct gcctgct
//
LOCUS       DATMGX010000172         3072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_147245, whole genome shotgun sequence.
ACCESSION   DATMGX010000172 DATMGX010000000
VERSION     DATMGX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147245"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VNR65_02790"
     CDS             <1..220
                     /locus_tag="VNR65_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17633.1"
                     /translation="QKKYMLVETEELIWVNVSARRTQSLSSCPMSGMGVRREKAALSS
                     GCKPHPATAPAGSNRSSHGGNEVAEAFD"
     gene            599..1687
                     /locus_tag="VNR65_02795"
     CDS             599..1687
                     /locus_tag="VNR65_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HWJ17634.1"
                     /translation="MGNLSTPKTVQKLQKALHAKAKAEAGYRFYALYDKISREDILAH
                     AYAQCRSNKGAPGVDGQDFADIDAYGVGRWLAELALALREESYRPDPIRRAFIPKANG
                     KLRPLGISTVRDRVCMTAAMLVLEPIFEADLPPEQYAYRPGRNAQQAVIEVDRLLFRD
                     HPEVVDADLADYFGSIPHAELLKSVARRIVDRRVLHLIKIWLECPVEETDDRGRKTRT
                     TEARDNRRGIPQGSPVSPLLANICMRRFVLGWNMLGLEQSLGSRIVTYADDLVILCRR
                     GKAEEALQQLRKIMSKLKLTVNEEKTRICRVPEGEFDFLGYTFGRMFSERTGQARLGY
                     RPSKKSIKRMVSARPKPPAMRFAASRVG"
     gene            2293..>3072
                     /locus_tag="VNR65_02800"
     CDS             2293..>3072
                     /locus_tag="VNR65_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HWJ17635.1"
                     /translation="MMSDSSRVMSTRLRSKIMSADDAAALVLHGANVGMSGFTGSGYP
                     KAVPFALAHRIEEAHARGEPFRIGVWTGASTAPELDGALAKVNGIDLRLPYQSDPITR
                     ERINAGIMDYIDIHLSHVAQFVWFGFLGHLDIALVEVAAILPDGSLVPSTSIGNNKTW
                     LDQADRIILEVNSWMNPALQGMHDIYYGTKLPPHRTPIPILAPGDRIGETTFRCDPSK
                     VIAVVETNAPDRNTTFAPPDQTSKAIAGHILEFLGHEVRRGR"
BASE COUNT          678 a          863 c          968 g          563 t
ORIGIN      
        1 tcaaaaaaag tatatgctgg ttgagacaga ggaattgatt tgggtcaacg tgagcgcccg
       61 gcgaacacaa tcactaagta gctgcccgat gtccgggatg ggtgtgcgcc gtgaaaaggc
      121 ggcgctttcc agtgggtgca agccccaccc ggcaaccgct ccagccggaa gcaatcggag
      181 cagtcacgga ggtaacgaag tggctgaagc cttcgattag cgtgtcacga attggtgaca
      241 gcgcgagtgt gcaggccgta acgcgagtga acgccgagca agcctcgaaa aggactatgt
      301 gcaggccgac ccgacaaccc tttcggggaa ggctgatacg gctgggtgaa gtagagcgaa
      361 gcagtacgcc cagccgctgc gccggggtag tggcgacagc atgtacacaa ggaaagcgca
      421 cgcaacacgg gaagccccag aacgtggtca gggatgacca accggacgcc cgcgagggac
      481 aggtcgggcg ctctggggtg gcggagaggt tcgtagtacc gctgaaaccg ggtaacgccg
      541 gtggagggaa gggacctcag ttcaagacaa acgcaacaag tggtgaggga tttggagatt
      601 gggcaaccta tcaactccga aaactgttca gaaactgcag aaggcgttgc acgcgaaagc
      661 gaaggcagaa gccggctata ggttctatgc cttgtacgac aagatcagcc gcgaggacat
      721 tctggcacat gcctatgccc agtgccgctc caacaagggc gcaccgggtg tggacggtca
      781 agactttgcg gatatcgacg cgtatggcgt agggcggtgg ctagctgaac tggcgcttgc
      841 gctccgggag gaaagctacc gaccggatcc tatcagaaga gcgttcatcc cgaaggccaa
      901 cggcaagctc aggccgctgg gcatctcgac cgtgcgggat cgggtctgca tgacggcagc
      961 gatgctggtg ctggaaccga tcttcgaagc cgaccttcca cccgaacagt acgcgtaccg
     1021 tcccgggcga aatgcccagc aggcggtaat tgaggtggac cggctgctgt tccgagacca
     1081 tccggaagtc gttgacgccg acctcgcgga ctacttcggg agcattcccc atgccgaact
     1141 tctaaagtcg gtagcgcgcc ggattgttga tcggcgcgtg ctgcatctga tcaagatatg
     1201 gctggaatgc cccgtagaag aaaccgatga tcgaggaagg aagacacgca cgaccgaagc
     1261 ccgggacaac cggcgaggca tcccgcaagg gtcacccgtc tccccactac tggcgaatat
     1321 ttgcatgcgc cggtttgtgc tgggatggaa tatgctcggg ttggagcaaa gcctcggctc
     1381 tcgtatcgtg acctacgccg acgatctcgt gatcctgtgt cggagaggca aagccgaaga
     1441 agccttgcag caactgcgca agatcatgag caagctgaag ctcacggtca acgaggagaa
     1501 gacacgaatc tgcagggtcc cggaaggtga gttcgacttc ctgggctaca cgttcggacg
     1561 gatgttctca gagagaaccg gccaggcgcg cctaggatac cggccatcga agaagagcat
     1621 caagcgcatg gtctcggcac gcccgaagcc cccggcgatg aggttcgccg catcaagggt
     1681 tggctgatgg cccgcgatct ctcggagtat aatggcaagg gactggcgcc gctgcgttgg
     1741 cactattgat gacgcgcccg ccgcgccgcc cgcggctgcg ccaacgatat ttcggttaag
     1801 atcgccatat atcggaacaa atcacgcttt gattgattgg ctgtttattg gcccggagat
     1861 taaatcgtga gtgatgacag cccgctagct cctttcgtct acgaaggcct ggatcctgtg
     1921 ccagaaccga aatccacaat tgcggaagtc tccgatgctg tgaaaggaac aatcaatcga
     1981 gttagcggcg cgatagaatc gggtcgaaag cccggcatgc cgttgagcat cttaagccat
     2041 atcgcgcgcg aagcacctct tggttcgctg ttaatcgcgt tcctgttggg agttgccgtc
     2101 gcccgccgcc gataatggtt tgggggcgcg ctacctgtcc gggggtgaac agatggtctg
     2161 ctgaccgctg tctctgcgcg tcgatgtcgg cgagggatag gtaaagcggc tgtcgttacc
     2221 cgcccgcgat tttagagaag gatcgcccgc gaaacgccgg aacagaagaa tagtttctat
     2281 ttcagaggac ttatgatgag cgacagctcg cgggtgatgt cgacgagact tcgctcaaaa
     2341 attatgtcgg ccgatgatgc ggccgccttg gtgctgcacg gcgcgaatgt cggcatgagc
     2401 ggctttaccg ggtcgggata tccaaaggcc gttccgtttg cgctcgcgca ccggatcgag
     2461 gaagcgcacg cgcgcggaga accgttccgg atcggcgtgt ggaccggggc ctcgaccgcg
     2521 ccggaactgg atggcgcact ggccaaggtc aacggtatcg acttgcgcct tccctaccag
     2581 tccgacccga ttacgcgcga gcgcatcaat gccgggataa tggactatat cgatatccac
     2641 ctcagccatg tcgcgcagtt cgtctggttc ggctttctcg ggcatctgga tattgcgctg
     2701 gtcgaggtcg ccgccatcct gccggacggc agtctagtgc cctcgacttc catcgggaac
     2761 aacaagacct ggctcgatca ggccgaccgc atcattcttg aggtcaattc gtggatgaac
     2821 ccggcgctgc agggcatgca tgacatctat tacggcacca agctgccgcc acaccggacg
     2881 ccgattccta tcctcgcgcc cggtgacagg atcggcgaga ccacgtttcg ctgcgaccca
     2941 agcaaggtga tcgccgtggt cgaaaccaat gcgccggacc gcaacacgac tttcgccccg
     3001 cccgaccaga cgtcgaaggc gatcgccggc catattctcg aatttctcgg gcacgaggtt
     3061 cgccgcggca gg
//
LOCUS       DATMGX010000173         3363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_190832, whole genome shotgun sequence.
ACCESSION   DATMGX010000173 DATMGX010000000
VERSION     DATMGX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190832"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..2197)
                     /locus_tag="VNR65_02805"
     CDS             complement(128..2197)
                     /locus_tag="VNR65_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWJ17636.1"
                     /translation="MTMTTSKITQHHLIRQACVYIRQSTPGQVRFNQESTERQYNLMN
                     KAQAFGWSQEQIRVLDGDLGQSGSAAANRTDFKILVGEVAMGQVGAIFSLEASRLARS
                     NQDWHRLLELCAITNTLVIDEDGCYNPADFNDSLVLGMKGAFAQAELHIIRARLHGGK
                     LNKAAKGELHFPLPLGFVFEGDKIVLDPDQEVQGAVRMIFSLFEQTGSAFGVVRRFKE
                     LALRFPRRAYGGAWDGKLVWGPLIHSRVTSILLNPSYAGVYVFGRYQSSKEIGPAGEI
                     AIRSRRTPQEAWRVMIRDHHQGYIDWDQFTANGARLSANRTNADVLPGPAREGLCLLQ
                     GMLVCGVCARRVSVRYKGNGGVYPMYQCGSRHRDGLAESCNLNLPAGPIDSAFAERLA
                     GAITPLRIELALAALNTLEERDRAIGAQWPMRIERAQYEADLAERRYDTVDPNNRLIA
                     ATLEQRWNEALQRLRDLEAELTNFEQQAMRAVTADQKRQILQLVEDFPRLWAAPTTTS
                     KDRKRILRLLVRDITVVKGPGQRVVSLQVRWQGGETETITVALPPNRADAVRYPEEFV
                     DRIRALAVVYHDDEIASLMIAEGCKSSTGKNLTPKMIGWIRCKHRIQAPKPPSGTLTV
                     CQASERYGVTSGVVYYWIEHGVVRAQQRKRNRPYAITVDDATDYCLRDWVAKSNHLPS
                     TSQTQTA"
     gene            2190..2387
                     /locus_tag="VNR65_02810"
     CDS             2190..2387
                     /locus_tag="VNR65_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17637.1"
                     /translation="MVIALLLRNGRRGGVEISRNHVGKNGARLCQVELDGREIHGDLS
                     KILVASNKLCVDRTNLVERIA"
     gene            3041..>3363
                     /locus_tag="VNR65_02815"
     CDS             3041..>3363
                     /locus_tag="VNR65_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HWJ17638.1"
                     /translation="MVVKQVIEPDLDPIFLEDSYGYRPGKSALDAVGVTRKRCWKYDW
                     VVEFDIKGLFDNISHELLLKAVRRHIQCKWALIYIERWLKAPMVKDGARIERDCGTPQ
                     GGVISP"
BASE COUNT          678 a          953 c         1054 g          678 t
ORIGIN      
        1 ggcgggcctg cgcgaggagc tcgtttcgaa gacgtaccgg cccgatccgg tgcggcgagc
       61 gacgatcccg aagccgggcg gcggccaacg ccctctcggc ataccgacgc attgcgcata
      121 atgcacctca tgcagtttgt gtttgggatg ttgacggcag gtgatttgat tttgcgaccc
      181 agtcgcgcag gcagtagtct gtggcgtcat cgaccgtgat cgcatagggc ctatttcgtt
      241 tacgttgctg agcgcggaca acgccgtgtt cgatccaata gtaaaccacc cccgaggtca
      301 cgccatatcg ctcgctggcc tggcaaacgg tgagggtgcc ggagggcggc ttgggagcct
      361 ggatgcggtg cttgcacctt atccagccaa tcattttggg ggttagattt ttacccgtgg
      421 agcttttgca tccttcggcg atcatgaggc ttgcgatttc atcatcatga tatacgacgg
      481 cgagtgcacg aatgcggtcg acaaactctt ctggataacg aacagcatcg gcccggttag
      541 gcggcaaggc gaccgttatg gtctcagttt cgcctccttg ccagcggacc tggaggctca
      601 ccaccctctg ccccggccct ttcacgaccg tgatgtcgcg caccaacaaa cgcaggatgc
      661 gcttacgatc cttggatgtg gtggtgggcg ccgcccatag ccgcggaaag tcttccacga
      721 gctgtaagat ttgccgcttc tggtcagctg ttacggcgcg catggcctgc tgttcgaagt
      781 tggtcagctc tgcttcaaga tcgcgaaggc gttgcaaagc ctcattccag cgctgctcga
      841 gagttgcggc gatcagccgg ttgttggggt ctacggtgtc ataacggcgc tcggcgagat
      901 cggcttcata ctgggcccgc tcgatccgca tcggccactg tgcgccgatc gcacgatctc
      961 gttcctccag agtgttcagg gcggccagag cgagctcgat cctcagcggg gtaatggcgc
     1021 cggccaaccg ctcggcgaat gcgctgtcga tcggaccagc gggaaggttc agattgcagc
     1081 tttcggccaa tccgtctcgg tgcctggagc cgcactggta catcggatag acgccgccgt
     1141 tccccttgta tctgacgctc acccggcgag cgcagacccc gcagacaagc ataccctgca
     1201 ggaggcacag gccttctctg gcgggaccag gcagcacatc ggcgttagtc cggttggcgc
     1261 tgaggcgcgc gccattagcc gtgaactggt cccagtcgat ataaccttgg tgatgatcgc
     1321 ggatcatcac ccgccaggct tcctgcggtg ttcggcgcga tcgtatggcg atctcgccgg
     1381 caggcccaat ctccttggaa gattgataac gtccgaagac gtagaccccg gcgtaggatg
     1441 gattgaggag aatgctggtc acgcgggagt ggatcagtgg cccccaaacg agctttccgt
     1501 cccatgcccc accataggcg cgccgcggaa aacgcagagc cagctccttg aagcggcgaa
     1561 caacgccgaa cgctgaaccg gtctgttcga acaaagagaa gatcatgcgg acagcgcctt
     1621 gcacctcctg gtcgggatcg agcacgatct tgtcgccttc gaagacgaag ccgaggggca
     1681 aggggaagtg caactcgccc ttggccgctt tattcagttt gcctccatgg aggcgcgcgc
     1741 gaatgatatg cagctccgct tgggcgaagg cgcctttcat gcccaggacc agactgtcgt
     1801 tgaagtcggc tggattgtaa caaccatcct cgtcgatgac gagggtgttg gtgatagcgc
     1861 acagctccag aaggcggtgc cagtcctggt tcgaacgcgc caggcgcgat gcctccaacg
     1921 agaagattgc gccgacttga cccatggcca cctcgccgac caggattttg aagtcggtgc
     1981 ggttggcggc ggcggatccg gactggccca gatcgccgtc cagaacacga atctgctcct
     2041 ggctccagcc gaaggcttgg gccttgttca tcagattgta ttggcgctcg gtgctctcct
     2101 ggttgaaccg cacctgaccc ggcgtcgatt gacggatgta gacgcaggcc tgtcgtatga
     2161 gatgatgttg ggtgatcttt gatgtcgtca tggtcatcgc gcttctcctc cgcaacgggc
     2221 ggcggggcgg cgtcgagata agccgcaacc atgtcggcaa gaatggcgcc cgcctgtgtc
     2281 aagtcgaact ggacgggcgc gagatccatg gcgatctatc caagatcctc gtggcgtcga
     2341 acaagctctg tgttgatcgc acaaatctcg tcgagcgcat cgcgtaggtc gtgcaagttg
     2401 ttgatgaatt gagcgacacg gccaaagtcg gcgttgcgca cataatttct gtgaggccgc
     2461 ccgccaggct ggctgaccga gagataatgc ttgggcccgt ggcccggacc gtcgacgcag
     2521 cgacagttgg accggccgca gcgcttgtag gtctcgatca gcgtcccgcg catcacttgc
     2581 tcgatcggag gcgtgtcccg cacaagttcg cggcgacgtt tgcgtaaggc ggaggtggaa
     2641 agatccttga gattcatcgg accgattcaa aaggccttcc gagagaatca gaggtcgagt
     2701 catcggtcaa gcccctcgtc acgatatttt agacaagcca caaactcggc gaggcagacc
     2761 aaagcaaaga catcgatgag caacggcgcg cactcaggcg ggggctgggc gccgggcggc
     2821 gcccaatcgg tccaatcgcg cggcatggcg atggagccca agctagggtg acgaaggctc
     2881 agcatctcct ggccagaaag ccgccggacc ttcagaacct caaacgttcg cccacgtagg
     2941 ggatgaaagg gatgggtaac ggtcgccgat cccccagatc gattggcaaa gagtgcagtt
     3001 cgcttggtgt gtgcccactg tgggcgatag aatcgcgcag atggtcgtca aacaggtcat
     3061 tgagccggat ctcgatccca tattcctgga ggactcctac ggctacagac cgggtaaatc
     3121 ggcgctggat gcagttggcg tcacgcgtaa gcgatgctgg aagtatgact gggttgtaga
     3181 gttcgacatc aaaggtctgt tcgataatat ctcgcacgaa ctattactca aagcggtccg
     3241 gaggcatata cagtgtaaat gggcgctaat ttacatcgaa agatggttga aagcgcccat
     3301 ggttaaggat ggagcgagga ttgagcgaga ttgcggtaca ccgcaagggg gcgtgataag
     3361 ccc
//
LOCUS       DATMGX010000174         2704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_14829, whole genome shotgun sequence.
ACCESSION   DATMGX010000174 DATMGX010000000
VERSION     DATMGX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14829"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VNR65_02820"
     CDS             complement(<1..220)
                     /locus_tag="VNR65_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWJ17639.1"
                     /translation="MTDFTSISELAPRLRRREISPVEITRGCLARIEKLNPSLNAFIT
                     VMSESGMEQARRAEAEIGRGEWRGALHGV"
     gene            363..1808
                     /locus_tag="VNR65_02825"
     CDS             363..1808
                     /locus_tag="VNR65_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ17640.1"
                     /translation="MSEMTIAPVATHGFFVDGRWREDGDVVEIHAPYDNNLIARVVQG
                     RREHAEAAIAASVKAFGTTRRLPAFERQRVLRQIAAFMTQRKDEFARTLAQEAGKPIK
                     AARTEVERGIFTFNVAAEESTRIYGEYLPLDWQESTAGRWGIVRRFPLGPIAGITPFN
                     FPINLVAHKVAPAIAAGCSMVLKPAPQTPLCSLLLAECVQQAGWPDGGLNVLPLSNDD
                     ASLLVTDDRIKLISFTGSAPVGWDIKRRAGKKKVILELGGNAPVIVHCDADLEYAAER
                     CVTGGFGYAGQTCISVQRILVEHSVYGRFTDIFVDLVKKLKTGDPLDESTDVGPLIRN
                     SDAVRTTAWVEDAVSAGARLLCGGRRTGQVVEPTVLTGTKPDMKVNCQEIFGPVVTVE
                     PYKDFDQALRQANNSAYGMQAGVFTRDAKLLFQAYEELEVGGVIAGDVPSFRIDQMPY
                     GGVKDSGLGREGLRYAIEEMTEPKLMVMNLR"
     gene            2139..>2704
                     /locus_tag="VNR65_02830"
     CDS             2139..>2704
                     /locus_tag="VNR65_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWJ17641.1"
                     /translation="MSQIFHRSTNTLSRVTIFGAVFVIATLGWMAMEIQRSPYVTYAG
                     VRKPQPVPFSHQHHVTGLGIDCRYCHTSVETSSFAGIPPTKTCMNCHSQIWTNAQLLE
                     PVRASYKSGESLQWTRVNQLPDFVYFNHSIHINKGVGCNTCHGPVDQMPLMYQQESLQ
                     MEWCLSCHRAPENNLRPRDQVFNMRYQQP"
BASE COUNT          590 a          920 c          676 g          518 t
ORIGIN      
        1 gaactccatg cagcgcccca cgccactccc cgcgcccaat ctcggcttcc gccctccgag
       61 cttgctccat cccagactcg ctcatcaccg taatgaacgc attgagtgaa gggttcagct
      121 tctcgatgcg agcgaggcag ccacgagtaa tctcaaccgg agagatctcc ctccgccgca
      181 gccgcggggc cagctcagaa atagaagtga agtcagtcat aaagccgagc caattgtaaa
      241 gctatctcct ctgtgtcctc cgcgtcctct gtggttttaa ttttttcaaa cccatcccgc
      301 gtcgcgctca tccaaaagtc ctataattga ccaatcggtt cgctgggggc caaagaaaac
      361 ttatgtccga gatgacgatc gcacccgttg cgacacacgg tttctttgtg gatggccggt
      421 ggcgggagga cggcgacgtc gtcgagatcc acgcccccta cgacaacaac ctcatcgctc
      481 gcgtagtgca aggccgccgc gagcacgccg aggccgccat tgccgcctcc gtcaaggcct
      541 ttggcacgac ccgccgcctt cccgccttcg agcgccagcg cgtcctccgc cagatcgccg
      601 cgttcatgac acaacgcaag gacgaattcg cccgcacgct cgcgcaggaa gccggcaagc
      661 ccatcaaggc cgcccgcact gaagttgagc gcggcatctt caccttcaac gtcgccgccg
      721 aagagagcac ccgcatctac ggcgaatatc tcccgctcga ctggcaggaa tccactgccg
      781 gacgctgggg catcgtgcgc cgcttccctc tcggccccat cgccggcatc acgccattta
      841 atttcccgat caatttagtc gcgcacaaag tcgcgcccgc catcgccgcc ggatgctcca
      901 tggtgttgaa gcccgcgccc caaaccccgc tctgctcgct gcttctcgcc gaatgcgtgc
      961 agcaggcggg atggcccgat ggcggactca acgtgctccc gctctccaac gacgacgcca
     1021 gcctcctcgt caccgacgac cgcatcaagc tcatcagctt caccggcagc gctcccgtcg
     1081 gctgggatat caagcgccgc gccggaaaaa agaaagtcat cctcgaactg ggcggcaacg
     1141 cccccgtcat cgtgcactgc gacgccgacc tcgaatacgc cgccgaacgc tgcgtcaccg
     1201 gcggcttcgg ctacgccgga caaacctgca tctcggtgca gcgcattctt gtcgaacact
     1261 ccgtctacgg cagattcacc gacatcttcg tcgacctcgt aaagaaactg aaaaccggcg
     1321 atcccctcga cgaatccacc gacgttggcc cactcatccg caacagcgat gcagtgcgga
     1381 caaccgcatg ggtggaagac gccgtcagcg ccggcgcgcg cttgctctgc ggcggcagac
     1441 gcacgggaca agttgtcgaa cccacggtcc tcaccggcac caagcccgac atgaaagtaa
     1501 actgccagga gattttcggc cccgtcgtca ccgtcgagcc ctacaaagat tttgatcagg
     1561 cccttcgcca ggccaacaac tccgcctacg gcatgcaggc cggcgtcttc acgcgcgacg
     1621 caaaattact cttccaggcc tacgaagaac tcgaagtcgg cggcgtcatc gccggagacg
     1681 tcccctcctt ccgcatcgat caaatgccct acggcggcgt caaagattcc ggcttgggcc
     1741 gcgaaggcct gcgctacgcg atagaagaaa tgaccgaacc caaattgatg gtcatgaacc
     1801 tgcgttaagc cgtaaccgtt tccgatttcc ttagtgaacc ttagtgtcct tagtgtttga
     1861 agcttttagg tttttcgctg gcgcgcagtt gaaaaactca acatttttct agtaacgttc
     1921 ccgtcgaaac ttttttcttt tccgaaacgc aaaaatccgc gacaaactcc gacgaaatat
     1981 ttataatttc gagtttcaca gacaactctg aaaaccagtg ggtggttttc tctatcgctg
     2041 taccaaacca gtattcaacc gaatcgcgga cgatcgaaca tagagcgatc gaacctagga
     2101 tgctccgccg ggtctcataa gccagacctc ataagggaat gtcgcagatt tttcatcgca
     2161 gtacgaatac tctgtcgcgc gtcactatct ttggcgcggt gttcgtcatt gctaccctcg
     2221 gctggatggc catggaaatc cagcgctccc cctacgtgac ctacgctgga gtccgcaagc
     2281 cgcagcccgt tcccttcagc caccagcatc acgtcacggg actgggcatc gactgccgct
     2341 actgccacac ctcggtcgag acctcgagct ttgccggcat tccgccgacg aaaacgtgca
     2401 tgaactgcca ctcgcagatc tggacgaacg cccagctcct cgaaccggtc cgcgccagct
     2461 acaaatccgg cgaatcgctg cagtggacgc gcgtcaacca gctccccgac ttcgtctatt
     2521 tcaatcacag cattcacatc aataaaggag tcggctgcaa cacctgtcat ggtccagtcg
     2581 accagatgcc gctgatgtac cagcaagaga gcctgcagat ggagtggtgc ctgagctgcc
     2641 atcgcgcgcc agaaaacaac ctacgtcctc gcgaccaggt cttcaacatg cgctaccagc
     2701 agcc
//
LOCUS       DATMGX010000175         2333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_396400, whole genome shotgun sequence.
ACCESSION   DATMGX010000175 DATMGX010000000
VERSION     DATMGX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396400"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VNR65_02835"
     CDS             <1..214
                     /locus_tag="VNR65_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17642.1"
                     /translation="SPRQQPFELSRQGFGSTVLTRVVPETLSGEARYGAGKDGIIWEV
                     EAPLHEVASLPSNWSATDTVAAVRAS"
     gene            complement(309..1031)
                     /locus_tag="VNR65_02840"
     CDS             complement(309..1031)
                     /locus_tag="VNR65_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17643.1"
                     /translation="MFEVIRDDLIWIMSWVLDRRPHGEEAFRLLFLIAFSVLATWLAI
                     YWKNLAYRSSSIRRRLVPNERYAGRYLQALWRNGELRYSFVNIFYNAKRRRYEVAGRT
                     YSPEGKELADFKSAYVVFPSEKDANIEFIWHAGNTSQGYTRMTLEDSDEDYIQGDGLV
                     ISFDAWPRAYPVRFKHLHDHHVRKALGVRSPTESSEEPEFVKRFHAAFGAHVMEGFGG
                     AAKGGAKAKAQPLMPNALVTQS"
     gene            1218..1541
                     /locus_tag="VNR65_02845"
     CDS             1218..1541
                     /locus_tag="VNR65_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17644.1"
                     /translation="MGISDEAWAVAAEKRCKGLIHLLGIGDLEAGDTSIRGRLSIIAQ
                     LKAAKRSEIARGQTGSWLYDVNRHLSICSCLRREYEALEEILDGSSPAPSQRKRADNV
                     IELRP"
     gene            2037..>2333
                     /locus_tag="VNR65_02850"
     CDS             2037..>2333
                     /locus_tag="VNR65_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17645.1"
                     /translation="MSARYGVLQVFSHEFGSKFASGYFVREIDRCLVTLKIAEKGTTE
                     NQSGQTAAHVRFVLNPGQKAGLDSEEGQSMNLTCSTNATALILDSGGKDALTAAQ"
BASE COUNT          521 a          639 c          670 g          503 t
ORIGIN      
        1 aagcccgagg cagcagccgt tcgagctatc gcggcagggt ttcggatcga ctgttcttac
       61 ccgggtcgtt ccggaaacat tgtccggtga agctcgctac ggtgctggga aggacgggat
      121 tatctgggag gtcgaggcgc ctctgcacga agtggcgtcc cttccgtcca actggtccgc
      181 aacggatact gttgcagcgg tgcgcgcgtc ttagagccct ctcttgatgg aatcaaaaac
      241 gggctccaac tttttatttt gtcgtgcgtc tttatcggaa aaccgcttcg cacttttccg
      301 gacgcactct agctttgcgt cacgagcgcg tttggcatca ggggctgcgc cttcgccttg
      361 gctccacctt tggcggcgcc gccaaagcct tccatcacat gcgcgccaaa tgccgcgtga
      421 aacctcttaa cgaattccgg ttcctcgctg gactctgttg gtgaacgaac gccaagcgct
      481 ttgcggacgt gatgatcgtg cagatgcttg aagcggaccg gataggctct gggccacgca
      541 tcgaatgaga tgacgaggcc atcgccttgg atgtagtcct cgtcggaatc ctccagcgtc
      601 atccgggtgt acccttgcga tgtattaccc gcgtgccaga tgaattcgat attcgcatcc
      661 ttttcggacg ggaaaacgac ataagccgat ttaaaatcgg ccagttcctt cccttcggga
      721 ctgtaggtgc ggccggcaac ctcgtaccgg cgccgcttcg cattgtagaa gatgttcacg
      781 aaggaatagc gcaactcgcc attgcgccag agcgcctgaa ggtagcgtcc cgcatagcgc
      841 tcgttcggca ccagcctgcg ccggatcgag gaggagcggt aggcaaggtt cttccaataa
      901 atggccagcc atgttgccag gacgctgaat gcgatcagga acaggagacg gaatgcctcc
      961 tcgccgtgtg gacgccggtc caagacccag ctcatgatcc agatgaggtc gtcccgaatg
     1021 acttcgaaca tgcatacccc gcagttggcc aggaatttcg tgaaattaaa ccgcggttca
     1081 aggcataatt tgctctgcga gcctatttgc ccaaggaagc tcggcgcgca ctcccatcct
     1141 atcgaactcc atttaccaaa acttaaagcg ctttctccta tcagccaatg agttgatttt
     1201 gggtgcagga ggcacttgtg ggcattagcg acgaagcttg ggcagtagca gccgagaagc
     1261 gctgcaaagg gttgattcac cttctcggaa ttggtgattt ggaggcaggc gatacgagta
     1321 taaggggacg cttgtcgatc atcgcgcaac tcaaggcggc caagcgctcc gaaatagcac
     1381 gaggtcagac ggggtcgtgg ctctacgatg tcaatcgcca cttgagcatc tgctcctgcc
     1441 ttcgaaggga atatgaggcg ctcgaggaga tccttgatgg cagcagcccg gcgccaagcc
     1501 aacggaaacg cgccgacaac gtcatcgagt tgcgtccgtg acaaagacca tgttgaccga
     1561 cagccagctg acgcaggctg tcgatttcga cggcggcagc gctatgcagg agggctcgga
     1621 ctcgaaatgc agaatgaact aagcccgtag ccgtaccggc gctggcgctc gtggacaaaa
     1681 gaaagacact cacaatcgag gacataacca ggtccattta agagcatgcg atcgtggttt
     1741 tctccccaaa ggccgctcgt gcaccataaa gttggacctt tcgggacttc gctgaccatg
     1801 gttggcgcgt tgtccagcgt cgtgtcagga aggcgttata tccgggtcgc tccagaagca
     1861 attcattcga ggagtggttt atgaaaagat ctaacccccg tttcgctagg ggcaagcatc
     1921 ccctaggaac gcaccgggta aaaataggag cacaccacag actcgcctcc gcttttgcgg
     1981 cacttttcgt cgcctgcgtt ttcaccggta gtttggctgc ttcaaccgag gctgcgatga
     2041 gcgctcgata tggcgttctg caagtattca gccatgaatt tggttcgaag ttcgcaagtg
     2101 gatactttgt tagagaaatc gatagatgtc tcgtcaccct gaagattgct gaaaagggga
     2161 ctaccgagaa ccaatcgggc caaacagctg cgcatgttcg cttcgtcttg aaccctgggc
     2221 aaaaggcagg tctggacagc gaggaggggc agtctatgaa cttgacctgc agtacgaacg
     2281 cgacggcgct cattctcgat agcggcggaa aagacgcgct cacggcagcc cag
//
LOCUS       DATMGX010000176         2187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_406589, whole genome shotgun sequence.
ACCESSION   DATMGX010000176 DATMGX010000000
VERSION     DATMGX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406589"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VNR65_02855"
     CDS             <1..738
                     /locus_tag="VNR65_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWJ17646.1"
                     /translation="RLKTAFLSHVSYELRTPLTTIIGFSDLLAEPVTGPLNTKQLDYL
                     NDIKTSSQMLLNIINDILDLAVIDAGAMDLKLAPVNVEDMIEAAELGVRERLSRAKVN
                     LDVHIAKGAGTVMADSNRLIQVIYNLLSNAIGFSPEDGTITLNCRREKNGVAISVQDN
                     GLGIPEEEQATMFDRFESRSKGSRHRGAGLGLSLVKSIVQLHKGRIDLRSTPGAGTTV
                     TVYLPDRHPALQQKDKPALRKAIAAQS"
     gene            1391..1489
                     /locus_tag="VNR65_02860"
     CDS             1391..1489
                     /locus_tag="VNR65_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HWJ17647.1"
                     /translation="MTANKVKVTGSECEASNGVIHAIDIVLIPPMA"
     gene            1569..1781
                     /locus_tag="VNR65_02865"
     CDS             1569..1781
                     /locus_tag="VNR65_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17648.1"
                     /translation="MSHKFTIGQTVHLAPSNVRKAIAGDYEVCHLMPASDYQSEPRYR
                     VKNLAERHERVVTESDLTLPQETANL"
BASE COUNT          521 a          585 c          586 g          495 t
ORIGIN      
        1 cgcttgaaga cggccttcct cagccacgtc tcctatgagc tgcgcacacc gctcacgacc
       61 attatcggct tttcggacct tctggccgag cccgtaacag gccccttgaa tacgaaacaa
      121 ctcgactacc tgaacgatat caagacatcg agccagatgc tcctcaacat catcaacgac
      181 attctcgatc tggccgtgat cgatgccgga gcgatggacc tcaagcttgc gccggtgaac
      241 gtcgaggaca tgatcgaggc cgccgagctt ggtgtgcggg aacgcttgtc gcgggccaaa
      301 gtcaatctgg acgtccatat cgcgaaaggg gccggaacgg tcatggccga ttccaaccgg
      361 ctcattcagg tcatctacaa tcttttgtcg aacgccatcg gcttctcgcc ggaagacggg
      421 accatcacgc tcaactgccg gcgcgagaaa aatggtgtgg cgatcagcgt ccaggacaac
      481 gggctcggca tccccgaaga agagcaggcg acgatgttcg accgcttcga gtcgcgcagc
      541 aagggctcgc gacaccgggg cgccgggctt gggctatcgc ttgtgaaaag tattgtgcag
      601 cttcataagg gccgtatcga tctgcgctcc acacccggtg ccgggaccac cgttacggtt
      661 tacctgcccg accgccaccc ggcgttgcag caaaaggaca agcccgccct tcgcaaagcg
      721 atcgcagccc agtcctgagc cagcttccgc tgcgtgaagt cgctgcttct cgcttgaagc
      781 cggacttggt caagaccgtc gcaagcgttc ttgacccgaa gcggtcttcg caagcgcgat
      841 cttggcaaca ctattcgagg gcctcgtggc ttgaaactct gagccggctt ggtggctgcg
      901 ggtctttcta ccggcgcgct tcataccgac catgaagaac aggccgtcgc tgttggggcg
      961 cttccatcca aggtgaggcg catgagctcc agataggaga gcccgcctcg ttttcgcgat
     1021 gatccgccgc ttttgcggac gcgacacttt gatggtctgg gtgatttgtc ttcttgcggt
     1081 atggagatac acacgaagaa taatgaggtc cagcaagcga ctaatgagga tgcttattgt
     1141 aattcaagga gtgaatttat tttattatat ttttttccaa aggcttgtgt tatacgattg
     1201 cactaacaat gtgtttatca tacgaaaatc aaaatatgcc atgttctaga taaacaagaa
     1261 aatccattaa ttatatttat tttagtctca aaaggaattt cagggtgagt tctgctctga
     1321 aaatagctgc cgccaatacg cggacatttt cgatcgaggg cgcagagacg ttgctcgatg
     1381 ggagccgtgc gtgacagcaa acaaggtgaa ggtgacgggc tccgaatgcg aggcatcgaa
     1441 tggcgtcatt catgccatag acatcgtgct catacctcca atggcataat taccggcgag
     1501 aggcggcgtg acttgcccgc aatttttttc cggtccggac gttacactct aaactggagg
     1561 aggaagattt gtctcacaag ttcaccatcg gccaaacggt ccatcttgcc ccaagcaatg
     1621 tgcgtaaggc tattgctggc gattacgagg tatgccacct catgccggcg tcggattatc
     1681 aatctgagcc gcgttaccgc gtaaagaacc tggcagaacg gcacgagcgc gttgtcacag
     1741 aaagcgatct gacgttgccg caagagaccg caaacctcta agaaaaactc tcacgctgca
     1801 caatgtaggc ggactatcaa tgtctttacc aggagttttg tgtgttaatt cttttaaaaa
     1861 attgaggttg tcgatgtctt ctcaacccta tcttagagca gcagatgatg ggacttatga
     1921 acttctggtc ccgttcggca aaggtgccgt ccatattctt ccttactgct tccatactga
     1981 ggaagacgca gcgaactggc ttgccagccg aaaaggccga gagctagtcc tgcacatgcg
     2041 accgaaaggt gaaaaaacac gaacgtctaa gccgtcattg tacgccccga acgaagcagg
     2101 cgatgttcct aagcgcatcg agtccctggt gggccgaaag gcggttcatg ttgcagcgag
     2161 aatgatttca agctgaagcc ctggctt
//
LOCUS       DATMGX010000177         2466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_369711, whole genome shotgun sequence.
ACCESSION   DATMGX010000177 DATMGX010000000
VERSION     DATMGX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369711"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VNR65_02870"
     CDS             complement(<1..913)
                     /locus_tag="VNR65_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWJ17649.1"
                     /translation="MTAYRLLFGDLIARLGWRFPALIALMTLVGLSEGTSIALLLPLL
                     SGIGFAPASNPGPAVAMLERGLALIGAVDTAGILAVIIAVATGQAALFILLNWWTARL
                     GRRYQSQRQLELFGAFMRARWIFIVGRKSGELTNVIVTESERLGMAFTISLSIISTLV
                     VMLIYFGLSLLIAWPVTLSLIAFVLLGALAMVRVYRKSYVAGRALAPLNAELQSTLSE
                     QFAGIKILKATASENRAVARLDPLLRKLERANALATFLPAMVRGLLEFFALVGLSIVF
                     ALGNKGMEVGLGNVVVVVALFARLFPRV"
     gene            complement(1239..2063)
                     /locus_tag="VNR65_02875"
     CDS             complement(1239..2063)
                     /locus_tag="VNR65_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ17650.1"
                     /translation="MTPPKSSGLKVEEHFGFGENWLSFLSTVNEQSIASAIENLARLL
                     PPEHIAGRTFLDIGCGSGLSLLAAERMGARGTLGVDIDPQCVAASRQLLQAWKGVGRI
                     EMRSVFDLDAKHDGLFDIVYSWGVLHHTGDLWRAIKCAAQLVRPKGYLLLAIYRRTPL
                     CGFWSIEKRLYVHGPGWFRSVAQTAFKALFKVGLLAQGRNPSAYIRTYRSKRGMDWHH
                     DVHDWLGGWPYQSATPDEVIGFVKELGFDPVRTFAHNAKAFGLFGSHCDEFVFVRS"
     gene            complement(2225..>2466)
                     /locus_tag="VNR65_02880"
     CDS             complement(2225..>2466)
                     /locus_tag="VNR65_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17651.1"
                     /translation="VPGHVVIMLKDREDAAGFIQIDRQPQFRVGDKIRVLEGLFSDCL
                     GLYDGMPDRDRVAVLLDLLGRKVRVLLNAEAVAAA"
BASE COUNT          629 a          725 c          652 g          460 t
ORIGIN      
        1 tcacgcgcgg aaacagccgc gcgaacagcg caacgacgac aacgacatta cccaacccca
       61 cttccatccc tttgttgcca agcgcgaaca cgattgagag gccaaccaat gcgaagaact
      121 cgagcagtcc acggaccatt gccggcagga aggtggcgag cgcgttggcc ctttcgagtt
      181 ttcgcaatag cggatcgagc cgtgccacgg cgcgattttc actggcagtc gctttgagaa
      241 ttttgatgcc ggcaaattgc tcgctcagcg tggactgcaa ttcggcatta agcggagcca
      301 gggctcgacc agccacatag ctcttccggt agacgcgcac cattgcgagc gcgcccaaca
      361 gtacaaaagc gatcaggctc agggtgaccg gccaggcgat cagaagcgac aatccaaaat
      421 agataagcat cacgaccaag gtcgaaatga tggataggct aattgtgaat gccataccca
      481 gtcgttcgct ttcggtaacg atcacattcg tcaactcacc cgattttcta ccaacgataa
      541 agatccatcg tgcgcgcatg aatgcgccaa acaactccaa ttggcgctga ctctgatagc
      601 gtcgcccgag tcttgctgtc caccaattga gtaagataaa aagcgccgcc tgtcccgtcg
      661 caaccgcgat gatgacggca agaattcccg cggtatccac cgcgccgatg agcgccaagc
      721 ctctttcaag catcgcgacc gccgggccag ggttgctcgc gggtgcaaag ccgatgccgc
      781 tcagcaatgg cagcagcaac gcaatggagg ttccctcgct aaggccgacc agcgtcatca
      841 atgcaataag cgccgggaaa cgccagccaa ggcgagctat aagatcgcca aacaagagac
      901 ggtatgcggt catatgataa tcatcaggat cgagatcaca gatcgggctc ggatgcctac
      961 tgcgtgaaaa gcttcctggc ccgatcgaga aaatagagca acatggagaa cggatatgcg
     1021 acgactccta gcaatttagc gcgatcgtcg accaattcgc cccaaatgat agcgaattgg
     1081 aaagtcagag accttagctc acttcgcgat taactgagag gcgccatcgg tcgaaccgtc
     1141 gtatctctta gaacggtcat tagatcgccc gccctatcct ggcccaaata tcaatcgcaa
     1201 ttttgcgaac tctttgggga tctaatcggt ctgagctttt aagagcgaac aaaaacgaac
     1261 tcatcgcaat ggctgccaaa gagaccgaac gctttcgcgt tgtgggcgaa ggtccgaacc
     1321 gggtcgaatc ccagttcttt gacgaagccg ataacttcat cgggcgtagc cgattgataa
     1381 ggccatccgc ctagccagtc gtggacatcg tgatgccagt ccatgccgcg cttcgaacgg
     1441 tatgttcgaa tataggccga aggattgcgg ccctgggcga gcagcccgac cttgaacaag
     1501 gccttaaacg ccgtttgcgc aacgctgcga aaccacccag gtccgtgtac gtaaaggcgc
     1561 ttttcgattg accaaaaacc acagagcgga gtgcgccggt atatcgcaag cagcaaatat
     1621 ccttttggcc ggaccaattg cgcagcgcac ttgattgcgc gccaaagatc gccggtgtga
     1681 tgaagaacac cccacgaata cacaatgtca aaaagcccat cgtgtttagc gtcaagatca
     1741 aacaccgagc gcatctcgat gcggccgacc cctttccacg cttgcaacag ctgtcgactt
     1801 gcggccacgc actgtgggtc gatgtcaaca ccaagcgttc cgcgcgcgcc cattcgctcg
     1861 gcggccagaa gagaaagccc cgagccgcaa ccgatatcga gaaaggtccg tccagcaatg
     1921 tgttcgggcg gcagcaagcg cgccagattc tcgatcgccg atgcgatgct ctgctcgttg
     1981 acggtcgaaa gaaacgaaag ccaattctcg ccaaagccga aatgctcttc gaccttcaaa
     2041 cctgatgatt ttggaggagt catgatagac ggtcgaggga gtcttcgttg cgcatcacga
     2101 accaagagca ccatgcaaag cgaaggttat tatcgtcatt tttcgtcccg ttcggacatg
     2161 ctaccgcagt ccaggtgaac agctccccta gacagacctc ggatgaattt acgccacagc
     2221 gaaactaggc tgcggccacg gcctccgcgt tcaacaacac gcgaaccttg cgcccgagca
     2281 gatcgagaag caccgcgacg cggtcgcggt ccggcatgcc gtcatagagt ccaaggcaat
     2341 cgctgaacaa gccttcaagg acgcggatct tgtcacccac gcgaaactgc ggctggcggt
     2401 cgatctggat gaagccggcc gcgtcctcgc gatccttcag catgatgacc acgtgacccg
     2461 gaaccg
//
LOCUS       DATMGX010000178         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_190635, whole genome shotgun sequence.
ACCESSION   DATMGX010000178 DATMGX010000000
VERSION     DATMGX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190635"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..>2003
                     /locus_tag="VNR65_02885"
     CDS             235..>2003
                     /locus_tag="VNR65_02885"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWJ17652.1"
                     /translation="MVVELQGRPGHHMIWGRPVLAAISPGAWGWAAAASALYFLAAWL
                     AIPLTTPEGVAQFWPAAGIAVGFLIISGRTACWPVAAGIAVASFLANALAGRSGPACY
                     AFALGNTGEALLMAALIEQWFPRPFELDRFGNVLGLFGVAALGAVAWQAVMAMMLELS
                     GYTAAPFVFLWSRLVWANMTGIVIVAPILIGLAASARKPLSQREVFEGTAVLAFHTIA
                     SAHAFALLPLGLGRWMLLAPFASQLPLLLWLSVRCGPLFAANGSLVLGLSILGSFTLQ
                     RGRFADANFSLDERLAATDFAILATAFVALAIAALIAERKDAELAASQSRARLKLSLE
                     AGKLGLCELDPKSGSFEVTKMARACFGLRENSPLSLGTITDALHPGDRELWQSQLQRV
                     CEEGTELDVECRTQAADGGVRWLHITGKTVGGGSPHPRLRVAGAVRDITEQKSIASER
                     ENAERLRWFVEQAPVAIAMFDRNMHYMAVSRLWRTDYALGEASIIGRSHYDLFPEITP
                     FWKDVHRRGLAGETLRDERDPFTHADGWVQWLSWEVRPWHDAGGAVGGIFAIAEWQVA
                     GKRYFTGIIRDISERKRQEQKVAL"
BASE COUNT          338 a          583 c          621 g          461 t
ORIGIN      
        1 taggaaagcc cgatctgcca atgacgcgct gctttaaata gcgacgctga ctttccttgg
       61 gcggtgttgc caagcctttt agacgaacct agctgtaaaa ttccagccaa aagcgtagga
      121 ccaaatcagg tgccttgttg actgctttgc cgggggcctt caaagaaatt ctgttcgttg
      181 ccggtcaatt ctggtcattg tattttcgtt tgaattcgga ggatcaggcg agacatggtt
      241 gtcgagctgc aaggcaggcc cggtcatcac atgatttggg ggcgacccgt gctcgcagct
      301 atttcgccgg gtgcctgggg ttgggcagcc gctgccagcg cgctttattt cctcgctgcc
      361 tggctcgcga tccccctcac gactccagaa ggggtggctc agttctggcc ggccgccggg
      421 attgcggtcg gttttctcat catttccggc cgaactgcgt gctggcctgt tgcggccggg
      481 atagcggttg caagcttcct ggcaaatgca ctcgccggcc gcagcggacc cgcctgctat
      541 gcttttgctc tcggcaatac cggagaggcg ctcttgatgg cggctctcat cgagcaatgg
      601 tttccgcggc ctttcgagct tgaccgcttc gggaacgtcc ttggactgtt tggggtcgcc
      661 gctctcggtg cggttgcctg gcaggccgtt atggccatga tgcttgaact ttcgggctat
      721 acggcggctc ccttcgtctt cctgtggagc cggcttgtgt gggcgaacat gaccggtatc
      781 gtcatcgtcg cgccgattct gattggtctg gcggcatcag cgcgcaaacc tctctctcag
      841 cgagaggtgt tcgagggcac agccgtgctc gcgtttcata cgatcgcctc ggcccatgcc
      901 ttcgccctcc ttccgttggg gcttggccgc tggatgctgc tggcgccatt cgcgagtcaa
      961 ctgccgctgc tactttggct ttctgttcgc tgcggcccgc tgttcgcggc caacggatcc
     1021 ttagtgcttg ggctttccat cctgggttcg ttcacgctcc agcgtggccg cttcgccgac
     1081 gccaactttt ctttagacga gcggcttgct gccacggatt tcgccatcct cgcaacggcc
     1141 tttgtggctc tcgcgattgc cgctcttatc gccgagcgca aggatgcgga acttgccgcg
     1201 agccaaagca gagcgcggct caagctcagc cttgaggctg gcaagctggg tttatgtgag
     1261 cttgatccca agagcggctc gttcgaagtc acgaaaatgg caagagcctg cttcgggctt
     1321 cgcgaaaact cacctctatc acttggaacc ataaccgacg cactgcatcc cggcgaccgg
     1381 gagctttggc aaagtcagct ccagcgtgtg tgcgaggagg gaactgagct tgacgtcgaa
     1441 tgcagaacac aagcagccga tggcggcgtg cgctggctcc atatcacggg caaaacggtc
     1501 ggtggcggct ccccgcatcc acggcttcgg gttgcgggag ccgtccgcga catcaccgag
     1561 caaaaatcta ttgcgtccga aagggagaat gccgagcggc tacgttggtt cgtcgagcag
     1621 gccccggtcg cgatcgcaat gttcgatcgt aacatgcact atatggctgt cagccgcttg
     1681 tggcgtaccg actatgcatt gggggaagcc agcatcattg gccgctccca ttacgatctc
     1741 ttccccgaaa tcacgccgtt ttggaaagac gtgcaccggc gcggactcgc gggtgagact
     1801 ctgcgggatg agcgggaccc atttacgcat gccgatggct gggttcagtg gctgagctgg
     1861 gaagtgcggc cctggcatga tgcgggcggc gccgtgggcg gcatcttcgc gattgcggaa
     1921 tggcaggttg cgggaaagcg ctatttcacc ggtattatcc gcgacatctc cgagcgcaag
     1981 cggcaggagc aaaaggttgc gct
//
LOCUS       DATMGX010000179         2423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_218520, whole genome shotgun sequence.
ACCESSION   DATMGX010000179 DATMGX010000000
VERSION     DATMGX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218520"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VNR65_02890"
     CDS             <1..229
                     /locus_tag="VNR65_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ17653.1"
                     /translation="EFEREMIRERTRAGLQEARAQGRVPGRKPKITAEQKKEIVEAVS
                     SGRKTPAEIGRLLKIHRGTVSRIVSQSRAGG"
     gene            941..2224
                     /gene="ltrA"
                     /locus_tag="VNR65_02895"
     CDS             941..2224
                     /gene="ltrA"
                     /locus_tag="VNR65_02895"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HWJ17654.1"
                     /translation="MAKTKRGTALCSLHHVIDMDWMREAFWLTRKDGAPGIDGVTAQD
                     YEANLEANLLGLLERIKSGRYRAPPVRRAYIPKADGSQRMLGIPTFEDKVAQRAVTMM
                     LEAVYEQDFLPCSYGFRPGRSAHQALRTLQSVLWAKRLYWVIDIDIRKYFDSISHSHL
                     RTFLDQRVTDGVIRRMIDKWLNAGAVENGLLRRTTEGSPQGGVISPCLSNVFLHYVLD
                     EWFETVVKPRLGGESLLVRFADDAIMAFDNLVDAQRVLAALGKRLARFGLTLHPDKTR
                     LVDFRPHMTESARHPETDGTNFDFLGFTHVWGRSRNGKSMVRQVTAKGRFARAVAAVS
                     DWCRRHRHEPIRDQHRHLSSMMRGHFAYYGVGGNIRRLRGFAHQVGRIWRKWLSRRDR
                     QSVVTWTRFNELLKRHPLPSAKIAHGYAAASESHS"
BASE COUNT          499 a          720 c          771 g          433 t
ORIGIN      
        1 cgaattcgag cgcgagatga tccgcgagcg cacgcgcgcc ggcttgcagg aagcacgcgc
       61 ccaagggcgc gttcccggta gaaagccgaa aatcaccgct gagcaaaaga aggagatcgt
      121 cgaagccgtt tcatccggcc gcaagacgcc cgccgaaatc ggccgcctgc tcaaaattca
      181 tcgcggaacc gtgtcgcgga tcgtttctca gtcccgcgcc gggggctgag catgcctaaa
      241 atccttagcg tacagtttgg ggaaattctg cttgcgacag gctaagccct ggaaaggggg
      301 acggaaatgt gacctgaaac ctttcgtagc ggtccagagg gttcgacgcc ctcccggtag
      361 aggcgcagcc cgccagccgg aagcgagtct tgcatgggtg gcggcgacgt cgctcgtgaa
      421 gcgtagacag cggatgctga agccgtgtgt cagcctcgat attttcctac ccgttgaggc
      481 cttcggtttg tggaacccgg gggcagcatc gaggagaccg ccaaaggcga ggtttcctcg
      541 gctcgaccgg ggtcccggag cagggcagag gcatgggacg gatcgccagg gaacctgtga
      601 gatcccgctc gcgtccacgg gcgcgacgag ccggaacgcg gaacgccggc tgaccaattg
      661 ttccaggccc ggcggcgacc tctccgtctg tcgggagcgc ctcggcgagc acgaatgaga
      721 gaggagcgcg tgggtcccgc gaccgaacca ataagggagc gagagtgcgt gagcgggaag
      781 tcgtagcgcc ctcatattac ctgggaaagc ggggaaccga gctcatcggg acccgtcgga
      841 gggaaggggg gcgtcatgag gagagaaccg ttagcctgaa acacgggagg ggacactgga
      901 ccccataaac ctgtccacga acggacaacg gatagccgaa ttggcgaaga cgaaacgggg
      961 aacggcgctt tgttcgcttc accacgtcat cgacatggat tggatgaggg aggccttttg
     1021 gctgacccgc aaggacggcg ctccgggcat cgacggcgtg acagcgcagg attatgaggc
     1081 gaacttggag gccaatttgt tgggtcttct tgagcgcatc aagtccggcc gctatcgtgc
     1141 gccgccagtc cgccgagcgt acatcccgaa agcggacggc tcgcagagga tgctcggcat
     1201 tccaactttc gaggataagg tggcgcaacg cgccgtgacg atgatgcttg aggcggtcta
     1261 cgaacaggat ttcttaccct gttcgtatgg cttccgccca ggccgctcgg cccaccaagc
     1321 gctccgcacc ttgcagagcg tcctctgggc caagcggctc tactgggtga tcgatatcga
     1381 catacgcaag tatttcgact cgatttcgca ttctcatctc cgaacgttcc tcgaccaacg
     1441 agtcacggac ggcgtcatcc gacggatgat cgataaatgg ctgaatgcag gagccgttga
     1501 aaacggtctc ctacgccgca cgaccgaggg ctccccgcaa gggggcgtga tctcgccctg
     1561 cctctcgaac gttttcctgc actacgtgct ggacgaatgg ttcgagaccg tggtgaagcc
     1621 gcgccttgga ggagagagcc tccttgtccg gttcgctgac gatgcgatca tggcgtttga
     1681 caatctcgtc gacgcccaac gcgtcttggc ggcactgggc aagcgtcttg cgaggtttgg
     1741 gctcacgctc catccggaca agacgcgcct cgtcgacttc cgcccacaca tgacagagag
     1801 cgcgcgacat ccggaaacgg atgggaccaa cttcgacttt ctcggcttta cccacgtctg
     1861 ggggcggtcg cggaacggca agagcatggt caggcaggtc acggccaaag gccgctttgc
     1921 ccgcgcggtg gccgcggtga gcgattggtg tcggcgacac cgacacgaac caatccgcga
     1981 ccagcatcgc cacttgtcat cgatgatgcg gggccacttc gcctactacg gcgtcggcgg
     2041 caacattcga cgactgcgag ggttcgccca tcaagtgggg cgaatatggc ggaagtggct
     2101 gtctcggcga gatcgtcaaa gcgtcgtcac ttggacgcgc ttcaacgaac tcttgaaacg
     2161 ccatcctttg ccgtcagcca agatcgccca cggttatgcc gccgcgagcg aatctcactc
     2221 atgaagaacc ggatgcggga attccgctcg tccgggtctg cgagggatgg gggcggcaac
     2281 gtccccatct actcggcgat gaccccgtgt ctgataactc tcaagatgtt agccgaattg
     2341 ccccccgcac cgtcaaggga caggccgcaa gcggtttcgg ctcaccactt cttgacaact
     2401 gagatcatat tatcaatctc ggc
//
LOCUS       DATMGX010000180         2156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_62989, whole genome shotgun sequence.
ACCESSION   DATMGX010000180 DATMGX010000000
VERSION     DATMGX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62989"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1574)
                     /locus_tag="VNR65_02900"
     CDS             complement(156..1574)
                     /locus_tag="VNR65_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ17655.1"
                     /translation="MTRGKATETAGATGGKRKGRRGAKRSKLTLDESRILKPDSLPDG
                     ARFKGYEDFIVQDIVIMPWTILYRRERWLLPSGETVVAALPKGVGGHFGPSLKRFVLS
                     QYHQGQVTIPRLAAHLTGLGIEISKRQIVRLLTSKQDAFLKENIEVLRAGLLSASWLT
                     ADDTGARHQARNGFCTHIGNDCFAFFATTGSKSRLNFLELLRAGRDDYVINEAALAYM
                     REHKLPQATIARFGDAEQRFADREAFMAHLQKLGFTDLTVLPNPIQIATEAALWGSIV
                     DQGLLKDAVIVSDGAGQFRIAEHALCWVHAERLIHKLDTFCKAHVQAKERVRARIWRL
                     YKDLKAYRQAPTPRRARQLERRFDSIFSIETGFATLDRLLARLRANKDELLMVLKRPD
                     IPLHTNGSENDIRCQVTRRKISAGTRSDAGRDARDAFLGLMKTCAKQAISFWDYLGDR
                     LAAPQAPAVPRLDAVVHQHAAA"
     gene            complement(1483..1857)
                     /locus_tag="VNR65_02905"
     CDS             complement(1483..1857)
                     /locus_tag="VNR65_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17656.1"
                     /translation="MMGASFVTGAKLRAPAFARLTGDSFGDSVGTWPNLIREISRLGL
                     EELKALLLQVLEDNARLKAENAALREEIARLKGLKGRPNLKPSGMEHALGLDPGEGDG
                     DSRRDRGQAQGPPRRQALQTHS"
     gene            1944..>2156
                     /locus_tag="VNR65_02910"
     CDS             1944..>2156
                     /locus_tag="VNR65_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17657.1"
                     /translation="MKALGTVLRITVNFEGANPKPESGRKPVAEFGLLRRRELHFNFV
                     VQETARSLRSGRNGKSTSLLGPSFASK"
BASE COUNT          410 a          623 c          706 g          417 t
ORIGIN      
        1 taatccgtgg cggagaaagc ctattcgcgg gttatgaaaa cgaagaggtt gccactcgaa
       61 gagggctgat aatggtgaga caccgcaaaa aatgctgagc aaaatcaata cgggttcttg
      121 tcccactttg gtaacagggg caaaagcccg ggcggtcagg ctgcagcgtg ctggtggacg
      181 acggcgtcga gccttggcac ggcaggcgct tggggcgccg cgaggcgatc gccgagatag
      241 tcccagaaac tgatggcctg cttggcgcag gttttcataa gcccaaggaa agcgtcgcgg
      301 gcgtcgcggc ctgcgtcgga acgcgttccg gcggagatct tgcggcgcgt gacctggcag
      361 cggatgtcgt tttccgaacc gttggtgtga agcgggatgt cggggcgctt caacaccatc
      421 agcagttcgt ctttgtttgc gcgaagccgt gccaaaagcc tgtcgagagt ggcgaagccc
      481 gtctcgatgg aaaagatgga gtcgaagcgg cgctcaagtt ggcgggcgcg gcgcggggtt
      541 ggcgcctggc ggtaggcctt gaggtcctta tagaggcgcc agatgcgggc gcggacgcgc
      601 tccttggctt ggacgtgcgc cttgcagaac gtatcgagct tatgaatgag gcgctccgca
      661 tgaacccagc agagcgcgtg ctccgcgatc ctgaactggc cggcgccatc ggagacgatg
      721 acggcgtcct tcaaaaggcc ctggtccaca atcgagcccc acaacgcggc ttcggttgcg
      781 atctggatcg gattgggcag gaccgtaagg tcggtgaagc cgagtttttg cagatgggcc
      841 atgaaggctt cgcggtcggc aaagcgctgc tcggcgtctc caaagcgggc gatggtcgcc
      901 tggggcagct tgtgctcgcg catataagcg agcgctgctt cgttgatcac atagtcgtcg
      961 cgcccggctc tcagcaactc aagaaaattc agccggctct tcgagccagt ggttgcgaag
     1021 aacgcaaagc agtcattgcc gatatgcgtg caaaagccgt tccgggcctg atgcctggcg
     1081 cccgtatcgt cggcggtcag ccaggacgcc gacaaaagcc ctgcgcgcag cacttcgatg
     1141 ttttctttga ggaaagcgtc ttgcttgctg gtcagcagcc gcacgatctg tcgcttggaa
     1201 atctcgatgc ccaagccggt caggtgcgcg gccaggcgcg ggatcgtgac ctgcccctgg
     1261 tggtattggc tcaagacgaa gcgcttgagg ctcggcccaa agtggccgcc gacgcccttg
     1321 ggcagcgcgg cgaccacggt ctcgccggat ggaagcaacc agcgctcgcg tcggtagagg
     1381 atcgtccacg gcatgatgac gatgtcctgg acgatgaagt cttcgtagcc cttgaagcgg
     1441 gcgccgtccg gcaggctatc cggcttgagg atgcggctct cgtcaagagt gagtttggag
     1501 cgcttggcgc cgcggcggcc cttgcgcttg ccccctgtcg cgccggctgt ctccgtcgcc
     1561 ttcccccggg tcaagcccga gggcatgctc catgccgctg ggcttgaggt tcgggcgccc
     1621 cttcaagccc ttgagccgcg cgatctcctc gcgcagcgct gcgttctctg ccttcagccg
     1681 ggcgttgtcc tccaaaacct gcagcagcaa cgccttcaac tcttcgaggc caagcctgga
     1741 aatctcgcgg atcagattcg gccatgtccc aacggaatca ccaaatgagt cccctgtgag
     1801 tctggcaaac gccggtgccc ggagttttgc ccctgttacg aaagaggcgc ccatcatggg
     1861 accgaaagcc ggttcttcga tcccaggttc ggttgcattg aattttgcgg tttggtccca
     1921 agcggcaggg aaacggcaag aaaatgaaag ccctcggaac ggttctccgc atcaccgtca
     1981 atttcgaagg cgcaaatccc aaacctgaga gcggccggaa gccggtcgcc gagttcggcc
     2041 ttctgcgacg gcgtgagttg cactttaatt tcgtggtcca ggagacggcg agatcgctaa
     2101 ggagcggtcg gaacggcaag agcacaagtt tgcttggccc gtcgttcgcg tcaaaa
//
LOCUS       DATMGX010000181         7160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_349620, whole genome shotgun sequence.
ACCESSION   DATMGX010000181 DATMGX010000000
VERSION     DATMGX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349620"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VNR65_02915"
     CDS             complement(<1..927)
                     /locus_tag="VNR65_02915"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ17658.1"
                     /translation="MIRDLPHLPLHPAGEQSPIEHGTVGNQHAPTHAAPGLWALLDRI
                     PRDCWPSLLRGDSGFGTEAVMRGAEQRGLPYLFKLRLTSNVKKLIKRTFPKDDWTNAG
                     QGWQGREDTLRLEGWSRQRQVVILRRRLKEGMAIAGRGADQLALSFVEIGPGAEAYEY
                     GVLVTSLNEEVLTLAQLYRDRADSENPFDELKNQWGWAGFTTRDLARCRTMARFIALV
                     YNWWNLFVRLAEPDKHLEAITSRPLLLSAIAERSRHARQTTLRVASSHAKAGWAAGVL
                     SGIARFLRELVQSAEQLTADQRWRRILAHAVRS"
     gene            986..1459
                     /gene="bfr"
                     /locus_tag="VNR65_02920"
     CDS             986..1459
                     /gene="bfr"
                     /locus_tag="VNR65_02920"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HWJ17659.1"
                     /translation="MKGEPKVIEYLIAGLRHELTAVNQYWLHYRLLDHWGYKALAEYW
                     RKESIEEMEHADDLMVRIIFLEGCPRMQLDPLHIGKTVTNILECDLRLELAARAPDEE
                     AATYCHAVRDYVSRHLFEKLMHEEEHIDFLETQLNLVDKLGLQLYAQHHIGTMDD"
     gene            1599..1799
                     /locus_tag="VNR65_02925"
     CDS             1599..1799
                     /locus_tag="VNR65_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17660.1"
                     /translation="MPYMLDGQKQRHRHPNHKEQVAIFGHTHFPYSQRHSGANKLGRK
                     NRLLIDVLMQSSIQKLRLMYAT"
     gene            3774..4952
                     /locus_tag="VNR65_02930"
     CDS             3774..4952
                     /locus_tag="VNR65_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWJ17661.1"
                     /translation="MILFTRSFLAAACMAILSSSCYAGGFQFDELWKRTKDEFSTRFY
                     KATGSLRICAANELKPWLEKDVIPAFKETEKAVNIREGDLVFGGSGVLVENWNQGNRD
                     RCDIIILGSDVAALRSGDYDKNKATTLAYSPIVVVGQKEKLDAARSYLGKKAGDALSC
                     ADLSGVAAKGRLGRILKQGQSSKLPVTSRLTLEMSTSNSGQSAFITCVYSTFDATTAK
                     EVDDALATKESEAGEKSLKDFMNTVKFDVESSSGVKDSFVQSPLGVGGEHLAIFTYES
                     YLPEIKKVASQNGIELEVIYPPIGILSNFPATIIAKEGTPAHDTANVFLKLAKSKSMQ
                     ESLLKYGLRPSVAGIAMPDYMPLNIGVGDSPRNRGDLKKFWDIVGSVPSVKDAKMDAF
                     "
     gene            complement(5062..5934)
                     /gene="ppk2"
                     /locus_tag="VNR65_02935"
     CDS             complement(5062..5934)
                     /gene="ppk2"
                     /locus_tag="VNR65_02935"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HWJ17662.1"
                     /translation="MNRALSAPPSGTPIDAVQLAKLVWEISDPATHEVIREAIYTRTL
                     FQLQCELVKLQRWVHAKKLKIVVIFEGRDAAGKGGMIKRITHRLDPRLCRVVALGTPN
                     ERERAQWYFQRYVTHLPTGGEIVFFDRSWYNRAGVERVMGFCSDREYEEFLATVPYFE
                     NMITRSGIILVKYWLSISLAEQRRRLMERLKSPFKEWKLSPMDIESVRRWDSYTKAKE
                     IMFQRTHRPEAPWWVVDAEDKQRARLNCMAHLLSQVPYRALPTRHLAMPELQDISPQD
                     RKRPLGACYVPMAY"
     gene            complement(6373..>7160)
                     /locus_tag="VNR65_02940"
     CDS             complement(6373..>7160)
                     /locus_tag="VNR65_02940"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWJ17663.1"
                     /translation="VLETDQAEGAHQLRVGLTRLRAAHRALNPRLDSPAFHHLEDDAR
                     TIARAVGELRDADVLIEDIYAPVAGAAPVQQGFDALHHALQSHRTAKQGSARECLRGG
                     PWSRLLLSLALWPAMLETNPSLQEQSIEDYAKQALRKRWKKVAKCGRAIESLEPEEKH
                     KMRRSLKKLRYMIEFFAPIFQSKDVKPFVKQLKALQDVFGYVNDVRMAEQIRTIAVEH
                     GEGPDCAVAAGIVLGTHEERAAEAWTHAKEEWQRLKSRDRFWR"
BASE COUNT         1607 a         2038 c         1934 g         1581 t
ORIGIN      
        1 ggatcggaca gcgtgggcga gaatccggcg ccagcgctgg tcggccgtca actgctccgc
       61 agattggacc agctcgcgca gaaagcgggc aatgcccgag agcacgcctg ccgcccatcc
      121 ggccttggcg tgcgaactgg caaccctcag cgttgtctgc ctcgcgtgcc ggctgcgctc
      181 tgcgatggcg gaaagcagca gcggccggct ggtgatcgct tccagatgtt tgtccggttc
      241 ggcgaggcgc acgaacaggt tccaccaatt gtagacgagc gcgatgaagc gggccatggt
      301 ccggcagcgg gcgagatcgc gggtggtaaa gccagcccag ccccattggt tcttcagttc
      361 gtcgaaggga ttttcgctat cggcgcgatc gcggtagagc tgggcgagcg tcagaacctc
      421 ctcgttgagg gaagtaacga gcacgccgta ttcgtaggcc tctgcgcccg gcccgatctc
      481 aacgaagctg agcgccaatt gatcggcgcc gcgtccagcg attgccatgc cttccttcag
      541 ccggcggcgc aggatgacca cttggcgctg acggctccag ccctccagcc gcaatgtgtc
      601 ctcccttcct tgccagccct ggccagcatt ggtccaatcg tctttaggaa aagtcctctt
      661 gatcagcttc ttaacgttag aggtaaggcg cagcttgaac agatagggaa gccctctttg
      721 ctcggccccg cgcatcaccg cttccgtacc gaagccgcta tcgccgcgca aaagcgacgg
      781 ccagcaatcg cgcggaatgc gatccaggag cgcccacaat cccggcgccg catgggtggg
      841 cgcatgctga ttgcctacgg tgccatgttc aatcggcgat tgttcacccg ctgggtgaag
      901 cggaaggtga ggcagatcgc ggatcatctg cttgagaatt gacgattctc aagcaaagca
      961 tcctcggcta actaccggat ttaggatgaa gggcgagccc aaagtcatcg agtatctcat
     1021 cgccggcctg cgtcacgagc tgaccgccgt gaaccagtat tggctgcatt accgcctgct
     1081 cgatcattgg ggctacaagg cacttgcgga atattggcgc aaggaatcga tcgaggagat
     1141 ggagcatgcc gacgacctca tggtgcggat catttttctc gaagggtgtc cgcgcatgca
     1201 gcttgatccc ctccatatcg ggaagaccgt aacaaatatc ctcgaatgcg atctcaggct
     1261 cgaattagcg gcgcgcgcgc cggacgagga ggcggcaact tactgccatg ccgtgagaga
     1321 ttacgtcagc cgccatcttt tcgagaaact catgcatgag gaggagcata tagactttct
     1381 cgaaacccag ctcaaccttg tagacaagct tgggctccag ctctacgccc agcaccacat
     1441 cggcacaatg gacgattgac taagtctgct acgaagccga cgccgccact agacttcctt
     1501 cgctgattac aatatttcct tcggccgcag taagactgcg ctacgatttt gttcttaaaa
     1561 gtacagcttt aacagcgatt gaaataacga ataaacgcat gccctacatg ctggacggcc
     1621 aaaaacaaag acaccgccat cccaaccaca aggaacaggt tgctatattc ggccatacgc
     1681 acttccccta ctcccaacgc cactctggag caaacaaact aggtagaaaa aaccgtttgc
     1741 tcatcgacgt tttgatgcaa tcaagcattc aaaaactccg cctgatgtat gcaacttgaa
     1801 cataagatca tgcaggcgaa gactatcgat cttcatgtat ttgacttcgt cagacagaaa
     1861 cttctcaaga tagcgtacct actgatagta ttaattcaac ttaaattttg aaaacgacag
     1921 gaaaacacaa aatattgcgg ttccacagcc aaacaggctc cctatatagt agtcaggcat
     1981 aaaaagcttc tagcgagcgc gtctaaaaac gcggcatttg tgggcaacgt catcctacgt
     2041 ccgaccgaaa gtcggagtgc agatctgata aaaatcaata tggtgcgacg catcactttt
     2101 gatcgccttc agcggcgagc ttgacagcta accgagtcaa gcgatttttc aaattttcaa
     2161 tgaaaatgaa aatcagttgt aatccatttg caacgggact agggtgcctc gctcgcccgt
     2221 tacagtacgt gagtaacaaa acctagcgtt caatgacatg acgatagctt taccaattag
     2281 gccttggatc ccatcgcccg tcttccaccc attcagcaat cgtcgcagca tctccggaaa
     2341 cggactgctt caggtcttgc gtaatccgtc cgagtggaaa tgcgccgtca cggtggcgct
     2401 tgtcttcttc gtagcttctc tcgctcttgc acctgcgctc acacgcgatc agaagcgttt
     2461 agcgtcagag atcaacacca aaactctcaa agccgcggcc gcaatccaaa tgtccccgaa
     2521 gctgccgctc cgcgtccaaa attgagtgct tcgacgctca tcacatatat ctttgccgat
     2581 gtcgcctgca aatctgagct ttagggcgaa tgtacggcga cggcaggctt atgcctgcaa
     2641 tcaactattc aattcggtaa cttgtatgta ttggaaactc actgccgggc tcgcgattgc
     2701 ccttttcgct gtttcttttg cggcggctct actcacatcg atcccgcata agaacaaggt
     2761 ctttcaaacg atctcctctt cccatcccgc tggaaatgca taccctgagt gccgaagaaa
     2821 taaaccggcg acccctccag ctcgcagcca ccgctccggg ccaagcgccg catcgatgag
     2881 ctgatgccgt gggggcctac gccgccagtc agaaacctac cgcagtggcc tgagaacgcc
     2941 gctaacgctt ggtggcctgc tgaagctctg gctatccggt cagagcgtat cgtcagggga
     3001 gacgataaag atccggaagc tcctcgcggc catagaagag agccagctaa agctcaagtc
     3061 aaggttgtga ttggacgttt gtcgagctga tggcggcttc ttaagaaaag gaaatagcgt
     3121 tatcctttgc ttgaatgtaa gttgaggaaa ggatcctttg cttgcataga gtttatcagg
     3181 cgtactctcg ggcgaagtgc ctaaccggtc caaccaccca agcgctccgc atccccttag
     3241 cgccaagctc actctcgaac gaccttaagg gccaatttcg gaaagtcccg cccgtaggcg
     3301 ggtcgtagat aacgccatcg attggcgcgt cctgcccacg aagagcgtgt tccgtatcag
     3361 gcactgcatc aggccgttca ttgagcaacc tgaacaaaaa tacatcacga aacatcagag
     3421 cgggaagggt cggatcgccc atctacgcct cgctcgtcga agccgaaagc cccgatgagt
     3481 tcaccctcat ctggtccgcc gctaggccga gtaatcaagc ctcgaacgcg ctccgcccct
     3541 aaaggcgggg tgctcttaag cgcctatcga taatatgcga tttggtcgcc attttaggtt
     3601 cgcgtcctcg acacgaacag gccgcgatcg atggtttggc atcgccaatg acgggcgagc
     3661 gctgtcatat atccttcata cagacctgac attagctttt gccttgagct gtgttctcgc
     3721 gatccaataa ggtcagacgt ggattgttgg tgccgctttc cagggggtgt ctcatgattc
     3781 tttttacgcg ctcgtttctt gctgctgcct gcatggcgat actcagcagc agctgctatg
     3841 caggcgggtt tcagttcgac gagttgtgga agcgcacgaa ggatgagttt tccacccggt
     3901 tctataaggc gaccggctcc ctgaggattt gtgcggctaa tgaattaaag ccttggctgg
     3961 aaaaggatgt cattccggct ttcaaggaaa ccgaaaaagc tgtcaatatt cgcgagggcg
     4021 atctcgtctt cggtgggtct ggcgtgctgg tcgagaattg gaaccagggc aaccgtgacc
     4081 gctgcgatat catcattttg ggcagcgatg tcgccgctct ccggagcggg gactacgaca
     4141 aaaacaaagc caccaccctc gcctattccc ccatcgtcgt tgttggtcaa aaagagaagc
     4201 ttgatgcggc tcgatcttac ctgggtaaaa aggccgggga tgctttgtct tgcgctgacc
     4261 tgtcaggcgt cgcggccaag ggccgtctgg gccgcatctt gaagcagggc caatcgtcca
     4321 agctgccagt cacaagccgg ctcacgctag aaatgtcgac gtccaactcc ggacagtcag
     4381 ccttcatcac gtgcgtttac tcgactttcg acgccacaac cgccaaggaa gtggacgacg
     4441 ctttggccac caaggaaagc gaagccggag agaagtcact caaagacttt atgaatacgg
     4501 tcaagttcga tgtcgaaagc tcttccggtg tgaaggatag cttcgtccag tcgccactcg
     4561 gcgtcggcgg agagcatttg gccatcttca cttatgaatc ctatctcccg gagatcaaga
     4621 aggttgcttc tcagaacgga attgagctgg aggtcattta tccgccgatc ggtattctga
     4681 gcaatttccc agcgaccatc atcgccaaag agggaacacc cgcccacgac accgctaacg
     4741 tattcctgaa gctggcgaag agcaaaagca tgcaggaaag tttgctgaaa tacggcttgc
     4801 gcccctccgt cgccgggatc gcaatgcctg attacatgcc cctcaacatc ggcgtggggg
     4861 acagcccgcg taatcgcggc gacctcaaga agttctggga cattgttggt tccgtgccct
     4921 cggtaaaaga cgcgaaaatg gatgctttct gaaccttgca agctcagcaa ggcagagttc
     4981 ccaaaagtgg cctgctgcca gcccttgtcg tgcgtcttta tcgaaaaacc gcatacactt
     5041 atttcctgaa agcgctttag ttcaataggc catcggaaca tagcatgcgc ccaaagggcg
     5101 cttccggtct tgaggcgaga tgtcttgcaa ctccggcatc gcaagatgcc gcgttggaag
     5161 agcgcggtac ggaacctggc tcaacaaatg agccatgcaa ttcagccgcg ccctttgctt
     5221 gtcttccgcg tcgacgaccc accagggcgc ttcgggacga tgcgtgcgct ggaacatgat
     5281 ttccttggct ttggtgtagc tatcccaccg cctgacggac tcgatgtcca tcgggctcag
     5341 cttccattcc ttgaacgggc tctttagacg ctccatgagg cggcggcgct gctccgcgag
     5401 cgatatggag agccaatact tcacgagaat gatgcccgag cgcgtgatca tgttctcaaa
     5461 ataagggacg gtcgccagaa actcttcgta ttcgcggtcc gagcagaacc ccatgacccg
     5521 ctcgacgccg gcccggttat accagctgcg gtcgaagaac acgatctcgc cgcccgttgg
     5581 cagatgcgtg acatatcttt gaaaatacca ctgtgcccgc tcgcgttcgt tgggcgtgcc
     5641 cagggccacg acgcggcaga gtcgcgggtc cagccgatgg gtgatgcgct tgatcatacc
     5701 gccttttccc gcggcgtcgc gtccttcgaa aatcacgacg atcttgagct tcttggcatg
     5761 gacccaccgt tgcagtttga cgagttcgca ctgaagctgg aaaagcgtcc gggtgtagat
     5821 cgcttcccga atgacctcat gcgtggcggg atcggagatc tcccacacga gctttgccaa
     5881 ttgcaccgca tcgatcgggg tcccgcttgg cggagcggat aatgcgcggt tcactgagag
     5941 ggctctgttt tcgagggcat tttcgcacgt catttcacgt taataacgcg tcacagaagc
     6001 ttcaagaatc gtttgcacaa ctaatattac gcaaacaatg cgagcgccac aataggttgc
     6061 aatttgtttg gtgaggcagg ccggcaagcg tcctgggatg ggtcgaatga tacggaccgc
     6121 cgtaagccac gacgcttttg cccatcgcgg tccttggggt aaaacaagta cttatattat
     6181 tgcttaaagc ttttttgtga acgaccccaa tatgtttaat cgtttgtaat ctcgcaggat
     6241 aatgtgcctg gagaagctcc aattgatgct atgaatcgaa cgatctgcgg ctggtatggg
     6301 cccccaatat tgagcggggc gtctgggcgc aacctcttca tctcgcgcat gcgcgtgacg
     6361 atgcccttgc cgtcaccgcc aaaagcgatc gcgggatttg agccgctgcc actcttcttt
     6421 cgcatgcgtc caggcttcgg ctgctctttc ctcatgggtg ccgagcacga tgcctgcggc
     6481 aacggcacag tccggacctt cgccgtgctc gacagcgatg gtacgtatct gttcagccat
     6541 ccggacatcg tttacatagc caaagacgtc ctgtaacgcc ttcagttgct tcacgaaagg
     6601 cttgacgtcc ttgctttgga agatcggtgc gaagaactcg atcatgtagc gcagcttctt
     6661 caaggagcgg cgcatcttgt gcttttcttc gggttcgaga ctctcgatag cgcggccgca
     6721 tttggcgacc ttcttccagc gcttccttaa ggcctgtttc gcgtagtctt cgatggattg
     6781 ctcttgcaac gacggatttg tctccagcat ggccggccac agcgcaaggc taagtagcag
     6841 ccgcgaccac ggcccgcctc gaagacattc gcgggctgaa ccttgcttcg ctgtgcgatg
     6901 agactgaagc gcgtgatgga gcgcgtcaaa tccttgctga accggagcgg cgccagcgac
     6961 cggggcgtag atgtcttcga ttaagacatc ggcgtccctg agttccccga cagcgcgggc
     7021 tattgtgcgc gcgtcgtcct caaggtgatg gaaggccggc gaatccaggc gcggattcaa
     7081 agcccggtgc gcggcacgaa gacgggtgag gcccacgcga agctggtgcg cgccttcggc
     7141 ttgatcggtt tccagcacca
//
LOCUS       DATMGX010000182         2282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_146840, whole genome shotgun sequence.
ACCESSION   DATMGX010000182 DATMGX010000000
VERSION     DATMGX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146840"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..800)
                     /locus_tag="VNR65_02945"
     CDS             complement(180..800)
                     /locus_tag="VNR65_02945"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWJ17664.1"
                     /translation="MAIRFWMAHSAVTIRRWRPPVIVTFVVLAALTALPADAETRRAR
                     PASPTEAAAPRHAGEPIMAIVSIKSQQVTFYDQDGWILRAPVSTGTKGRETPAGVFAV
                     LEKDLNHHSSLYDDARMPHMQRITWNGIALHGGPLPGYAASHGCVRMPFGFAEELFDK
                     TRIGMRVIISPDDAAPAEFSHPALFMPNTEEVAAAPARAEKLAREA"
     gene            complement(1434..1955)
                     /locus_tag="VNR65_02950"
     CDS             complement(1434..1955)
                     /locus_tag="VNR65_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17665.1"
                     /translation="MLRGAHGQHLEPTPACGDGEEATPSETAGPFYKPRSPHRTSLIE
                     PRDTARQMALSGHVLSTRCKPVARALVDIWHADASGRYDLQGFRYRGHLFTDAQGAYD
                     IATIVPGIYEGRTRHIHVRVQLPGGAILTTQLYFPGEKANAWDDLFKRTLLMRMAADA
                     ALLTATFNFVLRV"
BASE COUNT          444 a          686 c          689 g          463 t
ORIGIN      
        1 gcccgcgcct tggcctcgtc ggttttggcg ccggcgagca ccttgttggc gtaggccagc
       61 tcggcgtcgg cacgggcctt gagccccgcg agcttgcgca gcgacgtcgt gagcgatgcc
      121 gtctggcgcg ccgatatcgc ggcggctttc ttcgtctcat cagccgttat ggcggcctcc
      181 taggcctcgc gggcgagctt ctcggcacgc gccggcgcag cggcgacctc ctctgtgttc
      241 ggcatgaaca gcgccggatg agaaaactcg gccggggctg cgtcatccgg cgagatgatc
      301 acccgcatcc cgatccgcgt cttgtcgaac agctcctccg caaagccgaa gggcatccgc
      361 acgcagccgt gcgaggccgc atacccgggc agcgggccac cgtgcagcgc gatgccgttc
      421 caggtgatgc gctgcatgtg cggcatccgg gcatcgtcat agaggctgga gtggtggttc
      481 aggtccttct ccaggacggc aaagacgccg gctggcgtct cgcgtccctt ggtgccggtc
      541 gacaccggcg cccgcaggat ccatccgtcc tggtcataga aagtcacttg ctgggacttg
      601 atcgacacga ttgccatgat cggctcgcct gcatggcgcg gcgccgccgc ctccgttggg
      661 gacgcaggac gcgcccgtct cgtttcggcg tcggcgggca gcgccgtcag cgcggcaagg
      721 accacgaacg ttacaatgac aggaggcctc caacgccgaa ttgtcacagc ggaatgcgcc
      781 atccaaaatc gaattgccat gccatacccc ggttaaaatg ccagtcgacg ctcgcgctga
      841 tcaggtatcc cagattgcga tattaagatc cactgactct ccccaacaat ccaaggttgc
      901 ccactcgctc ctagggacta acggcacgtt cccatggcgg tcttccatga ttggacagcc
      961 ttcgcagcag gaagcgggct tgcggctgaa attggcctac cttcaccgtc tgaagctcaa
     1021 tccatggtag acagcaaagg gatccgaaag ccggagtact ggtctaacaa agtttttaag
     1081 ccgccttcgc tggttccggc tctgagagtc ggtcttgcgg ttacaccgtg gttacgcttg
     1141 ccgcgttcca cgaattcctg atctggcgtt ccattgcgct ggatttcaga attcaggcaa
     1201 cttgaggccc cgtcgcgtcg cgttctgaag cgcaaaatag cgtaatttct tgcacttttg
     1261 aaaatttctg gacaaaataa ttttttgatt cgtaatgatg ggatcgggtg ttcgagtcac
     1321 ccaagcggta ccatttgttt tcaatggctt agcccaagaa cgcctggaaa caagaccggg
     1381 acatgccaga ccgttttaca aattcggcgg aaatatttta aaaacccaac ctttcataca
     1441 cgtaagacaa agttgaaggt ggctgtgagg agggcggcat ctgccgccat gcgcatcagc
     1501 agggttcgtt tgaaaaggtc gtcccaggcg ttagcctttt cgcccggaaa ataaagctgt
     1561 gttgtgagga tggcgccacc aggcagctga acgcggacat ggatatggcg tgtgcggccc
     1621 tcgtaaatgc cagggacaat ggttgcgatg tcgtaggcgc cctgcgcgtc cgtaaagaga
     1681 tggccgcgat agcggaagcc ctgcaagtcg tacctgccgc tggcgtccgc gtgccagatg
     1741 tcgacgagtg cgcgtgcgac cggcttacag cgcgtgctca gaacgtggcc agagagcgcc
     1801 atctgccggg ctgtatcgcg cggctcgatg agtgaggtgc gatgcggcga tctgggtttg
     1861 tagaacgggc cagcggtctc gctcggcgtg gcctcctccc cgtcgccaca tgcaggcgtc
     1921 ggctcgaggt gttggccatg tgcccccctg agcatggtta aaagtacggc cgaaatacct
     1981 gcccatctca atagatttcg ccgcgatgtt ccgccaacca tgacgccgcc tgctccattc
     2041 acgcattttc agtaccgccg ccgatacgag cttctacaca gggagaagga ctgcactccg
     2101 gggcctgttt tgcgtaggcg atggcgtcat ctcaactcga cacccggtac gccgatcgcc
     2161 tgcctgatct tcgatatggg gccagcactc ccaattggag cgtcagcgcg aatgcgtcgc
     2221 caaagccgaa gctgagcacg agggacgcac gccgactgcg atggaaaagc gggtgagcca
     2281 ac
//
LOCUS       DATMGX010000183         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_443048, whole genome shotgun sequence.
ACCESSION   DATMGX010000183 DATMGX010000000
VERSION     DATMGX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443048"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /gene="zorA"
                     /locus_tag="VNR65_02955"
     CDS             complement(<1..1094)
                     /gene="zorA"
                     /locus_tag="VNR65_02955"
                     /inference="COORDINATES: protein motif:HMM:NF033914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage ZorAB system protein ZorA"
                     /protein_id="HWJ17666.1"
                     /translation="MPILMRSIVWNSLSRFIAFWLIVTAVTVGLYAVWASLPASILGE
                     IVAEGLSGDLTRVSSEPFAFSLAKALGCVGFGLLIAFGILYALAVSVSIGRARALIEG
                     YKSKKAFADDLDRVRERMEAHPLLGHAWKKFQESVIPGGRPVRNTQRPQSFFNYAMLR
                     EKCIGLKLMPGVPNYFVGTGLLLTFIGLVIALYRAAEGTQAAQLAEAGAGAAAMQRAL
                     QELLHAATFKFSTSIAGLAVSIILAFAFRLYAVRIEASLSDFCEALESKLNYLSPQSL
                     SVQIRDSLTAQLTQLKEINSEAFFTRFGKEAGPSINTALTNALSPLTQEIRTAVEQLD
                     HNSHAGVQELVKQFSDTLQGGAGSELRELAG"
     gene            complement(1189..1668)
                     /locus_tag="VNR65_02960"
     CDS             complement(1189..1668)
                     /locus_tag="VNR65_02960"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HWJ17667.1"
                     /translation="MSSLKFIYFTFQGRIDRRTWWLAFVLIHLGIASFNYVLSKLIND
                     DAPFLDGTWPNLVRLLGDRSGWITAVVFLVPQLAINTKRFHDRGISGWWWFVFLIPFL
                     VATGISISSLGGEDYPSPLAGWAQLICGFTATWTLITLGFLPSKKTASASLDQADRR"
     gene            2218..>2559
                     /locus_tag="VNR65_02965"
     CDS             2218..>2559
                     /locus_tag="VNR65_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17668.1"
                     /translation="MTTVTILDFAKEQPVAVEEPDVCAADRKVLVADVGGNHVKLLVS
                     GQSEHRKVVSGPSLTPELMMAGIKDAVADWDHDVVTIGYPGPVLDGRPLRDPHNLGAG
                     WVQFDFEAAFGR"
BASE COUNT          548 a          729 c          778 g          504 t
ORIGIN      
        1 cccgcgagtt cgcgcaattc cgagcctgcg ccgccctgaa gcgtgtcgga gaattgtttg
       61 acgagttctt gcacgcccgc atggctgttg tgatcgagct gttcgaccgc ggtgcggatc
      121 tcttgtgtga gcggcgagag cgcgttcgta agcgccgtgt tgatcgacgg ccctgcctcc
      181 ttgccgaagc gtgtgaagaa cgcctcgctg tttatctcct tcagctgagt gagctgcgcg
      241 gtgaggctgt cgcggatctg aaccgatagc gactgaggcg aaaggtaatt cagcttgctt
      301 tccagggcct cgcagaaatc gctgagcgag gcttcgatcc tgaccgcgta cagccggaat
      361 gcgaaagcca ggatgatcga aaccgcaagc ccggcgatgg acgtcgagaa tttgaaagtg
      421 gccgcatgca gcaactcttg cagggccctt tgcattgcgg cggcccccgc acccgcttcg
      481 gcgagttgcg ccgcttgcgt tccctccgcc gcccgataga gcgcaatgac aaggccgatg
      541 aatgtgagaa gcagaccggt accgacgaaa tagttcggca cgcctggcat gagcttgagg
      601 ccgatgcact tctcgcgaag catcgcatag ttgaagaagc tttgcggacg ctgagtgttg
      661 cggaccggcc tcccacctgg aatgacgctt tcttgaaatt tcttccaggc gtggccaaga
      721 agaggatgag cttccatccg ctcgcgcacg cggtcgagat cgtcggcgaa cgctttcttc
      781 gatttgtacc cctctataag cgcgcgcgct ctgccgatcg aaaccgacac agccagagcg
      841 taaagaatac cgaaggcaat caacaacccg aaaccgacac agcccagagc cttggccagc
      901 gaaaatgcaa agggttccga gctgacgcgc gtaagatcgc cactgagccc ttccgcaacg
      961 atctctccga ggattgacgc tgggagtgac gcccacacgg cgtagaggcc aaccgtaacg
     1021 gcagtcacga tcagccaaaa cgcgatgaag cgcgaaaggc tattccaaac aatgcttctc
     1081 ataagaatgg gcaaggggca ccccaaagta ccgtcagcga gaccttcagg gctgacacta
     1141 tctatggaga gcctcgtgcg aacctatcgt gcagcccgat cctgcagctt accggcggtc
     1201 cgcttgatca agggaggcag aagcagtctt tttcgacggc aagaagccga gagtgatcag
     1261 tgtccacgta gccgtgaagc cgcagatcaa ctgtgcccag cctgcgagtg gcgacgggta
     1321 atcctcgccg ccgagcgatg agatcgagat gcctgtcgca acgaggaagg ggatcaggaa
     1381 gacaaaccac caccagccgg atattccccg atcgtggaag cgcttcgtgt tgatcgcgag
     1441 ttgcggaacc aggaatacca ccgcagtgat ccaccccgaa cggtcgccaa ggaggcggac
     1501 gaggttcggc cacgtgccgt ctaggaacgg cgcgtcgtcg tttatgagct tcgatagcac
     1561 gtagttgaag cttgcgatcc ccaagtgaat aagtacaaag gccagccacc atgtgcgccg
     1621 gtcgatgcgg ccctggaacg tgaagtaaat gaatttcagg gaggacatgg cagctgctcc
     1681 tcgtttggcg ctggcacaaa tcgggtttgc cttcttgccc tggcaggacg aagcgggcga
     1741 cgagaataag tccaacacgc cgtctaggga tgcgcaacgc gtttgagtgc gctaagctga
     1801 agcgcgatga tagcttttgc gggtgagcgc aatcatgctt tacgatggac gaggcgatta
     1861 tatcaggcgg tatgagcctc ggaagacgcc ctcgcccgga atgacggctt ggctcgccct
     1921 cctattgttt tcggccgcga tgctttgggc gtcgacgggc tcttacaagt cttccgaacc
     1981 cgaccagacc tttcagcagg tcttttcctg gggctgaccg gcgtgtgcca gtgttgatgt
     2041 gtgggtgcgg ccgataatcg ccctctcatg acaaaccgct gaatacagga gggccggccg
     2101 cgtcatcgcg acaggcggcc gctgctgcgc ttaagtggct tcttgcggat cagcgcggtc
     2161 cttcgcatac taagaattgc accatatccc ggtttttgaa agaacctgat cagtcccatg
     2221 acgacagtaa caattctcga tttcgcgaaa gagcagccgg tggccgtaga ggagccggac
     2281 gtttgcgcgg ctgataggaa ggtcctcgtt gccgacgtcg gtggcaacca cgtcaagctc
     2341 ctcgtcagcg ggcaaagcga gcaccggaaa gttgtctccg ggccctccct cacgcccgaa
     2401 ttgatgatgg cgggaatcaa ggacgccgtt gcggattggg accacgatgt tgtcacgatc
     2461 ggttatcccg gccctgttct cgacggccgc cccttgcggg atccgcataa tctgggagca
     2521 ggctgggttc agttcgattt tgaggctgca ttcggccgg
//
LOCUS       DATMGX010000184         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_364970, whole genome shotgun sequence.
ACCESSION   DATMGX010000184 DATMGX010000000
VERSION     DATMGX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364970"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VNR65_02970"
     CDS             complement(<1..283)
                     /locus_tag="VNR65_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17669.1"
                     /translation="MSPPDSNADSRFPSAEQHSAPVEAVGAQGHGVALIGGRKVYIPF
                     TLPGETISALIAGTKGDAIAIDSPSPDRISPICKHFGDCGGCSLQHWRQV"
     gene            complement(258..983)
                     /locus_tag="VNR65_02975"
     CDS             complement(258..983)
                     /locus_tag="VNR65_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWJ17670.1"
                     /translation="MRLDLLLVQRGMVETRSKARDLIKRGLVRMRGRTDLKASLEVSI
                     EEPVEVLETQGYFARSAWKLLAALNHFALSPAGLTCLDVGASTGGFTQVLLERSAARV
                     FAVDVGRDQLHPSLRTDARITSWESTDVRTLTPEQFPTPIEAVTCDVSFISLLKVLPS
                     ALPLAAKGAWLVALVKPQFEVGRALIGKGGIVKDEAAKLAAVQRVTDHIEAAGWAVLG
                     TLPSPIKGQDGNEETLVAARLER"
     gene            1219..1683
                     /locus_tag="VNR65_02980"
     CDS             1219..1683
                     /locus_tag="VNR65_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HWJ17671.1"
                     /translation="MKQNQPHYSIGIYQIMMLVRTYLSNSSIEGVGIFAAEPIKAGDV
                     IWRLEPKFDVFFTESDIEELPPHMQDFIARYSYPHMTKPGIWVLESDNGRFMNHSEAP
                     NTDFTGFEQGFAIRDIAAGEEITCNYHEFDSTFQGWFPSMAKAANGEAHAHP"
BASE COUNT          450 a          597 c          562 g          445 t
ORIGIN      
        1 ggacttgccg ccagtgctga agcgagcagc caccgcaatc cccaaaatgc ttgcagatgg
       61 gcgatatgcg gtcgggtgaa gggctatcga tggcaatcgc atcgcctttt gtgcccgcga
      121 tcagtgcgct gatcgtttcg cccggcaagg tgaaggggat atacactttc ctgccgccga
      181 tgagtgccac gccgtggcct tgcgcgccta cggcctcgac aggcgcgcta tgctgctcgg
      241 ccgatggaaa acgcgagtca gcgttcgagt ctggcggcga caagcgtttc ttcattgccg
      301 tcctgtcctt ttatcggcga tggcaacgtt cccaaaaccg cccatcccgc cgcctctata
      361 tggtccgtaa ctctttgaac ggcggccagc ttcgccgcct catccttgac gattccgccc
      421 ttgccgatga gcgcccggcc gacctcgaat tgcggcttta ccaaagcaac aagccaagct
      481 cccttcgcgg cgagggggag tgctgacggc agcaccttta gcagagaaat gaagcttaca
      541 tcgcatgtca ccgcctcgat cggcgtcggg aattgttcag gcgtcagtgt acggacatcc
      601 gtgctttccc aggaggttat ccgagcgtcc gtccgcagcg acggatgaag ctgatcgcgg
      661 ccgacatcca cagcaaaaac gcgggcagca ctgcgctcca gcagaacctg tgtaaaacct
      721 ccggttgagg ccccgacatc gagacaagtt agccccgcag gcgatagggc aaagtggttc
      781 agcgcggcga gaagcttcca ggcgctgcgc gcaaaatagc cttgtgtttc aaggacttcg
      841 acaggttctt cgatggatac ctccaggctg gccttcaggt ccgtccttcc tcgcattcgg
      901 acaaggccgc gcttgatgag gtctcgcgct ttggaacgcg tttccaccat tccacgttgc
      961 accaaaagga gatcgagtcg cattttcaca agtaatttcc gaggctctgg agccgcccaa
     1021 atatgtatgt tgcaaatccc ttgaattgcg gcaatcatcg ataatctgtg ttgttagcag
     1081 caaccagagc aagcgctgtt ttcccaacct cgctcaagga tgttgaaaaa gcacatgtga
     1141 gagacgacag cctcttcgcg gccgaggtcg cacagaaggc cgtgagcgca atacttggcg
     1201 caatgccgtc aagcttcaat gaagcaaaat caacctcact actctattgg gatctaccaa
     1261 atcatgatgc ttgtccgcac ctatctctcg aatagttcga tcgagggagt aggcatcttc
     1321 gctgctgagc ccattaaagc gggcgatgta atctggcgtc tcgagccaaa attcgatgtc
     1381 ttctttacgg agtctgacat agaagaactt cctccccaca tgcaagactt catcgcccgc
     1441 tactcttatc ctcatatgac taagccgggc atttgggtgc tggagtccga taatggccgc
     1501 tttatgaatc atagtgaagc gccaaacacc gatttcacgg gttttgagca aggcttcgcc
     1561 atccgcgata tcgccgccgg tgaggagatc acctgcaact atcacgagtt cgattcaacc
     1621 ttccagggat ggttcccgtc catggcgaag gcggcaaacg gagaggcgca cgctcacccc
     1681 tgatacagcg ccaacggagc tagcgccggc cgcttttggg cgggccggcg tttttgcgtt
     1741 cgcgatgctc tcttagccgc cgggtcgatg cgcggcaaaa aaggggattg gcaagggacg
     1801 aatcgcacct tatcttatag tcgtgttgag cataagggca ctcaggcgaa gcttgaggtg
     1861 acagcctatt tttgagccct aataatgctc aaagtgagca taaccccgaa agggacggag
     1921 gatcacatgg cattcttaga tagcggagtt cagccggtcc gtgggcgcaa cctttatact
     1981 gctccggcga tcgacctcga tatcggcttc gaacccgtgc gccaccattt cgacgccgcc
     2041 ttcgtccgcg aagc
//
LOCUS       DATMGX010000185         2271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_315134, whole genome shotgun sequence.
ACCESSION   DATMGX010000185 DATMGX010000000
VERSION     DATMGX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315134"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VNR65_02985"
     CDS             <1..679
                     /locus_tag="VNR65_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWJ17672.1"
                     /translation="ETFSTAADNQTSVEVHVLQGERPMARDNRTLGKFHLTGLPPAPR
                     GLPQIEVTFDIDANGILNVNAKDMATGKDQKITITSSSGLSKDEVDRMAKEADAHSAE
                     DKTKRDEVEARNQLDGMVYSIEKMLREHGDKISGQERSDVENALADAKKALEGTDAAA
                     MNKARETLTSASHKLAEAMYKASSTQTPPTGGPPPGGDGHAAGGEQKKKDEGVIDAEY
                     VDVDEKK"
     gene            796..969
                     /locus_tag="VNR65_02990"
     CDS             796..969
                     /locus_tag="VNR65_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17673.1"
                     /translation="MNGQSYMLSFNNDDGRWYLITAGIDGRMKAIPVISDEFGFVAHA
                     VIPMGDGGQASIN"
     gene            1367..>2271
                     /locus_tag="VNR65_02995"
     CDS             1367..>2271
                     /locus_tag="VNR65_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWJ17674.1"
                     /translation="MPTTANKDKDYYGILGVKKTASADEIRKAFRKLARKYHPDVNPG
                     DKSAEEKFKTLSEANDVLSDPKKRKIYDQVGFYSDNIDAATAEAYARGGGSGGFGGGG
                     AGGGGARTYTQEVPFDFSGFDFNDYAEGAGAAGRKSGGGGFRDIFSGMFGGGRQESMQ
                     HPAPGDDLEYQVNVGFWQAIKGTEMQLTIPRREVCSSCHGQGFLESPGQCPECHGKGQ
                     ITQKSGSMKFNVVCPRCGGSGKNRTVCHVCHGEGRVERSDSLKVRIKAGTRDGQRIRL
                     PGRGNAGSMGAPAGDLYIIVRVGEHP"
BASE COUNT          537 a          658 c          739 g          337 t
ORIGIN      
        1 ggaaaccttc tcgacggcgg ccgacaacca gacttcggtc gaggtacacg tgctgcaggg
       61 tgaacgtccg atggcgcgcg acaaccgcac tctcggcaag ttccacctga ccgggctgcc
      121 tccggctcca cgtggcctgc cgcagattga ggtgacgttt gacatcgatg ccaacggcat
      181 cctgaacgtc aatgcgaaag acatggcgac cggcaaggac cagaagatca cgatcacgtc
      241 gtcttcgggt ctgagcaagg acgaggtcga tcgcatggcg aaggaagccg atgcgcactc
      301 ggcggaagac aaaaccaagc gcgacgaagt ggaggcccgc aaccagctcg acggcatggt
      361 gtacagcatc gagaagatgc tgcgcgagca cggagacaag atctccgggc aggagcgcag
      421 cgacgtggag aacgcgctgg ccgatgccaa gaaggcgctt gagggcaccg acgcggcggc
      481 gatgaacaaa gcgcgggaaa ctctgacttc cgcttcgcac aagctggccg aggccatgta
      541 caaggcgagc tcgacgcaaa cgcctccaac gggcggtccg ccgccgggtg gagacggcca
      601 cgccgcaggc ggcgagcaga agaagaaaga cgaaggcgtg atcgacgccg agtacgtgga
      661 cgtggacgag aagaagtaac tagcagctag caactagcaa ctagtttttc gagtacgggg
      721 cgcgcaagcg cccctgctca caacgggtag cgaaaagcga agggcgagaa gcgaagagcg
      781 ataatgagat tcgaagtgaa cggtcagtcc tacatgttaa gtttcaacaa cgacgacgga
      841 cggtggtacc tgataaccgc cggcatcgac ggccggatga aagcgattcc ggtgatcagc
      901 gatgaatttg gcttcgtcgc gcacgccgtc attccgatgg gcgacggcgg acaggcgagc
      961 attaattgag actcgagtcc cgctctgcgg gacgacataa gatagctcac cgcttcaggg
     1021 ggcgtgaatg atcagagtgc cccgggcacg gcacaattcg accacaagaa ggttttgtgc
     1081 cgcccctgcg ggactccacg tcgttgcgac aattactaaa cccgaggagc gagagaccca
     1141 ttgacttgtc atcctgagcg gaggcggcct gaagttggcc gccggagtcg aaggatctgc
     1201 ggtatcgcct gagcagagga ccgcagatcc ctcgactgcg ctcggctctc gcctcgctcc
     1261 gctcaggatg acaatgcgaa aataggattt agcgaaaagc gagcagcgaa gagcgaaaag
     1321 cgattacgga gagctccagc tgaaagctga cggctgatag ccaaccatgc ccaccaccgc
     1381 caacaaagac aaagactact acggcattct cggggtgaag aaaactgcct cggcggacga
     1441 gatccgtaag gcgtttcgca agttggcgcg caagtatcac cccgacgtga atccgggcga
     1501 caagtctgcc gaggaaaagt tcaagacgct ctccgaggcc aacgacgtcc ttagcgatcc
     1561 caagaagcgc aaaatctacg accaggttgg gttctactcc gacaacatcg acgcggccac
     1621 ggcggaagcc tacgcgcgcg gaggtggctc tggcggcttc ggcggaggcg gcgccggcgg
     1681 gggtggagcg cgcacctaca cccaggaagt tcctttcgac ttcagcggct tcgacttcaa
     1741 tgactacgcc gagggcgcgg gcgcagccgg ccgcaaaagc ggtggtggcg gattccgaga
     1801 catcttctct ggcatgtttg gcggcggacg tcaggagtcg atgcagcatc ccgcaccggg
     1861 cgacgatctg gaataccagg tgaacgttgg cttctggcag gcgatcaagg gcacggagat
     1921 gcagctcacc attccacgcc gcgaagtctg ctccagctgc catggccagg gcttccttga
     1981 gagccccggc cagtgtcccg agtgccacgg caagggacag atcacgcaaa agagcggcag
     2041 catgaagttc aacgtcgtat gcccacgctg cggcggcagc ggcaagaacc gcacggtgtg
     2101 ccatgtgtgc cacggcgaag ggcgcgtcga gcgcagtgac tcgctaaaag tccgcatcaa
     2161 agctggaacg cgcgacgggc agcgcatccg cctgccgggc cgcggcaacg cagggagcat
     2221 gggcgcacct gcgggcgatc tctacatcat cgtgcgcgtg ggtgagcatc c
//
LOCUS       DATMGX010000186         3892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_190252, whole genome shotgun sequence.
ACCESSION   DATMGX010000186 DATMGX010000000
VERSION     DATMGX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190252"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /gene="tcmP"
                     /locus_tag="VNR65_03000"
     CDS             complement(<1..442)
                     /gene="tcmP"
                     /locus_tag="VNR65_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HWJ17675.1"
                     /translation="MPIPFKFDEVGIWSELKLEIVEKYGSAYTAAFANQRGLKKFYID
                     AFSGAGVHISKRSGGQIEGSPARALKTSPPFDGFFFIDMDAQKTAYLRTLCAGRSDVH
                     IETGDASKYLIQTLLPTIKYEDYKRALCLFDPYGLHLEWRAMEMA"
     gene            complement(820..1752)
                     /locus_tag="VNR65_03005"
     CDS             complement(820..1752)
                     /locus_tag="VNR65_03005"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWJ17676.1"
                     /translation="MDLSYAQNLEDYHLSLAFAGQTTGTYVDVGAGHPIADNVSFWFY
                     ERGWNGMVVEPQPELAALYQRLRPRDIVVRGLVGRHCGEIDFYAVERLHGLSTTSQSH
                     AEKAKAFGVDYQTVRMPVTTLADLCERHDVGSIDFLKIDVEGAEGDVLFGGNWKRFRP
                     KVIVAEAVAPMSSEPAWQDWEPFLLAQGYRFVLFDTVNRFYVAQEHPEIMARLPSERA
                     PWHAVRHMYEIGRAPENKRHPDHALAQDLARGFWASLPYLDTELITSLLGRARRIAKP
                     DEFAALASTVDTEAFRAALGRVACGYDGGQIVDE"
     gene            2188..3411
                     /locus_tag="VNR65_03010"
     CDS             2188..3411
                     /locus_tag="VNR65_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ17677.1"
                     /translation="MTLFRSTAALVLALGATFATPALAQKKYDTGASDTEIKVGQTIP
                     LSGPASAYGGIGKVQAAYVRMINEQGGVNGRKINLIQYDDAYSPPKTVEQVRKLVESD
                     EVLTTFQIIGTPPNAAVQKYLNEKKVPQLLASTGATRFTDPKNFPWTIAFNPNYQSEA
                     HIYAKYILANHPDAKIAILYQNDDLGKDYVKGLKDALGAKASSMIVAEKSYELSDPTV
                     DSQMVTLKASGADLFYNITTPKFGAQAIKKAAELGWKPVQILDINATPVSQTLVPAGV
                     ENAKGIISVNYGKDPLDPQWADDEGMKRYKAFMAKYAPAEDANSGIATYGYSTAALLV
                     QILKQCGDDLTRANIMNQATNIKGYAADLALPGMTISTEPDDWRINKQFQMMKFDGQR
                     WVLFGPILTDEFKTN"
     gene            complement(3579..>3892)
                     /locus_tag="VNR65_03015"
     CDS             complement(3579..>3892)
                     /locus_tag="VNR65_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17678.1"
                     /translation="QAHKFPEVTIGLSLLCSLPANIIERGLIDGTGEMPIIFARSLDY
                     SWETAMSLLFLGAPDFKLSAQRLDSLKARFSRLSVQTAQSVIRLYRSRKAPDGGKSLE
                     R"
BASE COUNT          842 a         1144 c         1113 g          793 t
ORIGIN      
        1 cggccatctc cattgctcgc cattccaggt gaagaccgta aggatcgaag aggcagagcg
       61 ctcgcttata gtcctcgtac ttgatcgtag gcagaagcgt ctggattaga tatttcgagg
      121 catcgcctgt ttcgatatgg acgtcactac ggccagcaca gagcgtcctc agataggcag
      181 ttttctgcgc gtccatatcg ataaaaaaga agccatcgaa aggcggtgac gttttcagcg
      241 cacgggctgg actgccctcg atctgcccac cactacgctt cgagatgtgg acgccggctc
      301 cactgaacgc gtcgatatag aattttttca gacctcgttg gttggcaaag gctgctgtat
      361 aggccgagcc gtatttttca acgatctcga gctttagctc ggaccaaatg ccaacctcat
      421 cgaatttaaa tggtatcggc acaggcgcta atcatgtgtt gctgccacgt cttcacgctg
      481 aacgactcga tcttgcgtcg gcgaaagccg ggcgttccat gacggctgag cccgattaga
      541 gccgcttcaa tcgccatagc accctcccga acgatcgcta gttcatcaag cccgcctagc
      601 gtcttacaac ttcaggtaat tttcaaccag cgattgcttg gtgtccgggc gttacttgat
      661 ggacacgaac gacaccgccg agcccgccac catggtggac ggaagcaaag aggaggcggg
      721 gtacgccgga gcctgtcatc gggccgcgct ttgcgcggac ccgttgggct gacccgcccg
      781 gccttccact accacgacca ttgccgggct cgcggtccct cattcatcaa cgatctgccc
      841 gccgtcatag ccgcacgcaa cgcgtccgag cgccgcacga aatgcttcgg tatcgaccgt
      901 ggacgcaagt gctgcaaact cgtcgggttt tgcaattcgg cgtgcgcgac ccaagagcga
      961 cgtgatgagc tcggtgtcga gataaggcag gctcgcccag aatccgcgcg cgagatcctg
     1021 cgccagcgca tgatcgggat gtcgcttgtt ttcgggggcg cggccgatct cgtacatgtg
     1081 gcgaaccgcg tgccacggcg cccgctcgga cggaaggcgc gccatgatct cgggatgttc
     1141 ctgcgcgaca tagaagcggt tgacggtgtc gaacagaacg aagcggtagc cctgggccag
     1201 aaggaacggc tcccagtcct gccatgccgg ctccgacgac atcggtgcta ccgcttcggc
     1261 gacgatcact ttgggtcgaa agcgcttcca attgccgccg aacagcacat cgccttcggc
     1321 gccctcgacg tcgatcttga ggaagtcgat cgagcccaca tcgtgccttt cgcataaatc
     1381 ggccagggtg gtcacgggca tgcgaacggt ttgatagtcg actccgaagg cttttgcttt
     1441 ttccgcatgg gattggctgg tcgtcgagag cccatgcaga cgctccactg catagaagtc
     1501 gatttcgccg cagtggcgtc cgaccaggcc acgcaccacg atatcgcgcg gccgcagccg
     1561 ctggtacagc gccgcaagtt caggctgtgg ctcgacgacc atgccgttcc agccgcgctc
     1621 gtagaaccag aatgacacat tgtccgcaat cggatgcccg gcgccgacat cgacgtaggt
     1681 cccggtggtc tgtcccgcga aggcaagcga aagatgatag tcctcaagat tctgtgcgta
     1741 ggataggtcc attcgcatcc tcggctctct ttggcgtgct gatgcatctt cagccgacca
     1801 gccagcgcgc gcagtggctt gtcggcattc tgtcggtttc ttcattctgc tccaattttc
     1861 ggaacttggc gtcaaggctc tctgcgaaaa catcgaataa tagcggtctc aggtccatcg
     1921 ccatacaggc aggccgaagg cggagcggct cctgtcttgc gcaccacgct tatcggcata
     1981 cagcggccgt gccgctgata ggtgagcccg aaccaggatg accaggatgc gaccgccaca
     2041 cggcggcgca ttctgtcgtg gcattgctta tggttcccgg attttcggaa acccgtcctc
     2101 tgcgcttttc atcgcgcgtt tttttagtat aggctgacta aatgacggag cggcgaagta
     2161 ccgcgaccgc tttttgggga ggaaggcatg actctattca ggtcgacggc tgctctcgtg
     2221 ttggcgctcg gcgcgacatt cgcaacgccc gcactcgcgc agaagaaata cgataccggc
     2281 gcgagcgaca cggagatcaa ggtcggtcag accatccctc tcagtggacc ggcttccgcc
     2341 tacggtggca tcggcaaggt gcaggccgcc tatgtccgca tgatcaacga gcagggtgga
     2401 gtcaacgggc gcaagatcaa tctcattcag tacgacgatg cctacagtcc gccgaagacc
     2461 gtcgagcagg tgcgcaagct tgttgaaagc gacgaggttc ttaccacctt ccagatcatc
     2521 ggtacgccgc cgaatgccgc ggtccagaaa tatctgaacg agaaaaaagt gccgcagctt
     2581 ctggcctcga ctggtgcgac gcgcttcacc gatccgaaga attttccgtg gacgatcgct
     2641 ttcaacccca actaccagtc ggaagcccat atctacgcga aatacattct tgccaaccat
     2701 ccggacgcga agatcgccat tctctatcag aacgacgatc tcggaaagga ttacgtcaag
     2761 gggctgaagg acgcgcttgg ggcgaaggct tcgagcatga tcgtcgccga gaagtcctac
     2821 gaattgtccg atccgaccgt tgactcgcag atggtgacgc tgaaagcttc cggcgccgac
     2881 cttttttata atatcacaac gccgaaattc ggcgctcagg cgatcaagaa agcggccgaa
     2941 ctcggctgga agcccgtcca gattctcgat atcaacgcga cgccggtcag ccaaacgctg
     3001 gttcctgccg gcgttgagaa cgccaagggc atcatttcgg tcaattacgg caaggacccg
     3061 ctcgacccgc aatgggccga cgacgagggc atgaaacgct acaaggcttt catggcgaaa
     3121 tatgctccgg ccgaggacgc caacagcggc atcgcgactt acggctactc aaccgcggcg
     3181 ctgcttgtgc agatactcaa gcaatgcggc gatgatctca cgcgcgccaa tatcatgaac
     3241 caggcgacga acatcaaggg ctacgccgct gatctcgcgc tgccgggcat gactatctcc
     3301 accgaaccgg atgattggcg catcaacaag caattccaga tgatgaaatt cgacggccag
     3361 cgctgggttc tgtttgggcc gatcctgacg gatgaattca agacgaactg aacgacttgc
     3421 ttaagagcgg acatcgaatg gcagccattc gatgtgaccc gcactccgtt cgcggcaaac
     3481 tggttcagat tcgcccgatc gccagcggtg gatttgggta gcggagattg atttatatgc
     3541 ctgggggaac cgcgtaagca cttaactggc gcactcgatc agcgttcgag cgacttgccg
     3601 ccgtcagggg ccttgcgcga ccggtacaat ctaatgacgc tctgcgccgt ctggacactc
     3661 aggcgggaaa atcttgcttt gagcgaatca agtcgttgtg cggaaagttt gaaatcgggc
     3721 gcgccaagaa acaggagcga catcgcggtc tcccacgaat aatcaagtga cctggcaaag
     3781 ataatcggca tttcgccggt gccgtcgatc aatcctcgct caatgatgtt tgccggcagg
     3841 gagcataaca acgacaggcc tatcgttact tccgggaatt tgtgggcctg cg
//
LOCUS       DATMGX010000187         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_14556, whole genome shotgun sequence.
ACCESSION   DATMGX010000187 DATMGX010000000
VERSION     DATMGX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14556"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /gene="pyrF"
                     /locus_tag="VNR65_03020"
     CDS             <1..671
                     /gene="pyrF"
                     /locus_tag="VNR65_03020"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWJ17679.1"
                     /translation="AIPKAVCKIGLELLMAGGVELVKKLASSGVSVFVDAKLFDIGNT
                     VERATASVADLGAAFLTVHAQDRRTLEAAVRGRAGWALKLLGITLLTSANPRDLSEQG
                     ITLSSRDLVLQRAAFAADAGFDGVVSSAWEARDLKATFCERLALVCPGIRPASETGIS
                     ADDQTRTATPGGALRAGADYIVVGRPILRASDPSAAAQTIIAEMATALRELQVQASSP
                     EKAS"
     gene            complement(755..1886)
                     /gene="prfB"
                     /locus_tag="VNR65_03025"
     CDS             complement(join(755..1825,1827..1886))
                     /gene="prfB"
                     /locus_tag="VNR65_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942918.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWJ17680.1"
                     /translation="MRPETLAIVDEIKQSLSLLRGIFDWDKATARLASLNAIVEDPTL
                     WDNPQRAQKLLRERQHLDTALTAYSQLDARLNDATGLIELGEEENDHEVVNDAEASLA
                     TLRTEVQKREVEALLSGEADANDTFVEVHAGAGGTESQDWTEMLLRMYMRWADEQGYK
                     VELIESNAGEEAGLKSATIQVKGQNAYGWLKTESGVHRLVRISPYDSNARRHTSFASV
                     WVYPVVDDAIDIAVNESDCRIDTFRASGAGGQHVNTTDSAVRITHTPTGIVVQCQNER
                     SQHKNRATAWTMLKARLYEMELKKREEAAQAAAASKTEIGWGHQIRSYVLQPYQMVKD
                     LRTGVVSTNPPAVLDGDLTQFIEAALAQRVHGGGPAAVADLD"
BASE COUNT          399 a          646 c          695 g          475 t
ORIGIN      
        1 tggcgatccc caaggctgtg tgcaagatcg gtcttgaact gctaatggcg ggcggcgtgg
       61 aactcgtcaa aaaactcgct tcgtccgggg tttccgtctt cgtggatgcg aaactgttcg
      121 atattgggaa taccgtcgag agggcaacgg caagcgttgc ggatctcggt gcggcgtttc
      181 tcacggtgca cgctcaggac aggcggaccc tcgaagcggc tgtgcgcggg cgggcgggat
      241 gggctctgaa gctgctcggg attacgcttc ttacaagcgc gaacccgcgc gatctgagcg
      301 agcaggggat cacgctctcg tcgcgcgatc tcgtgctgca gcgcgcggca tttgcggcgg
      361 atgcaggttt cgacggcgtg gtctcctcgg cgtgggaggc gcgcgacttg aaggcgacgt
      421 tctgcgagcg gcttgccttg gtctgtcccg gcattcggcc agcatccgaa acaggaataa
      481 gtgctgacga ccaaacgcgg actgcgacgc caggcggtgc tctgcgagcg ggggctgact
      541 acatcgtcgt tgggcggccg atcctccgcg cgagcgatcc ctctgccgct gcccagacga
      601 ttattgcgga aatggcgaca gcgctgcgag aattgcaggt gcaagcttcg tcgcctgaga
      661 aggcctcttg agcggtatca gcgatcgcaa ccgcttcttc tcagcgattt gctaagaccc
      721 cggataagcc caaagcgctc ttgacgagta cgctctaatc caaatcagcg acggcagccg
      781 ggccgccgcc gtggacgcgc tgtgcgagcg cggcttcaat gaactgcgtc aaatctccat
      841 ccagcacggc ggggggattc gtgctcacga cgccggtgcg caggtccttc accatctgat
      901 agggctgaag cacataagag cggatctgat gcccccagcc tatctccgtc tttgaagcgg
      961 cggcagcctg cgcggcctct tcccgcttct tcagctccat ttcgtaaagc cgcgctttca
     1021 acatcgtcca tgccgtcgcg cggttcttat gctgcgaacg ctcattctgg cactggacga
     1081 cgattccggt cggagtatgc gtgatacgca cagcgctgtc ggtggtattg acgtgctggc
     1141 ctccggcgcc gctcgcccgg aaagtgtcaa tgcggcagtc gctttcattc acggcgatat
     1201 cgatagcatc gtcgacaacg ggatagaccc agacagacgc gaaactcgta tggcggcgcg
     1261 cgttggaatc gtagggggaa atgcgcacca ggcgatgaac gccggactcc gtcttcagcc
     1321 agccgtaagc gttctgcccc ttgacctgga tcgttgccga cttcagccca gcctcttcgc
     1381 cggcgttgct ttcgattagc tcaaccttgt acccttgctc gtcggcccag cgcatgtaca
     1441 tgcgcagaag catctcggtc caatcctggc tttcggtgcc gcccgctccg gcatgcactt
     1501 cgacgaacgt gtcgttggcg tcagcctcgc cggataggag cgcttcaacc tcgcgtttct
     1561 gaacctcggt tcgaagggtg gcgagtgaag cctccgcgtc gttcacgacc tcatggtcat
     1621 tttcttcttc gccgagttcg atcaagccag tggcatcgtt gaggcgggca tcaagctgtg
     1681 aataggcggt cagcgctgta tccaggtgct gccgttcacg aaggagtttc tgagcgcgct
     1741 ggggattatc ccaaagggtc ggatcttcaa cgatcgcatt caggcttgcg agacgagccg
     1801 tggctttatc ccagtcaaag atacctccgc aagagcgaga gggattgctt gatttcgtcg
     1861 acgatggcga gtgtctcagg gcgcatatcg agttccgtct gtggtcacgg ccgggatcgc
     1921 agggtgacct ggttcggcca gctcattgag gacgtgcgta atcattttat taggttgcgt
     1981 aaacatgatt gtggcgcatg ttagagcaat ccgcctttag tcggaatcga ttaaaggcgg
     2041 taagttgatc gaccctaaag tgcttgagcg gcttatccgc gtcctaatgg gcgcgggcca
     2101 tcctagtaca accctccagc gcgcggctcg tgtccgtaag gcgggtagct ccctgcgcca
     2161 ggaggaggcg attgcggagg cgcaccccat tggccttgtg cggggggtgc ccccc
//
LOCUS       DATMGX010000188         2605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_364904, whole genome shotgun sequence.
ACCESSION   DATMGX010000188 DATMGX010000000
VERSION     DATMGX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364904"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VNR65_03030"
     CDS             complement(<1..637)
                     /locus_tag="VNR65_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17681.1"
                     /translation="MFGLFCFVLAVLASPFKSKSRLEAENAVLRHQLIVLRRKVQGRI
                     RLRNNDRWFLIQLYRWFPSILQVVTIVQPETLVRWHRAGFRRYWRWKSRPRGGRPQID
                     ADLRALIRRMSMESPLWGAPRIHGELLKLGFEVAQSSVAKYMVKRRGPPSQGWRTFLR
                     NHAPDIAAMDLFIVPTIGFDLLYAVIVRLGRRDLVWINVTTNPTAEWIARQL"
     gene            773..1042
                     /locus_tag="VNR65_03035"
     CDS             773..1042
                     /locus_tag="VNR65_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17682.1"
                     /translation="MEQYRDLPVEKDTKISKATMHKSVYQRFDLTKAFEYDAWIPYRP
                     STLSNHIDFARYYEPGAVFPATSLSTATEMVYDPETRDQFGPAVS"
     gene            complement(1245..1550)
                     /locus_tag="VNR65_03040"
     CDS             complement(1245..1550)
                     /locus_tag="VNR65_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HWJ17683.1"
                     /translation="MKRNLYAEVSTRIVAELERGAAPWIKPWSATAGMNTPCNAGTNR
                     PYSGSNVVLLWMAQTAGYRTPRFLTFKQTCHREIHHGQNGRTKNEELQKKLALMESE"
     gene            1953..2204
                     /locus_tag="VNR65_03045"
     CDS             1953..2204
                     /locus_tag="VNR65_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17684.1"
                     /translation="MLIQKHDESHIVELEDGSRWRIWPSDIAATLQWLPTTELRVSAI
                     SDEFCSHTLIDQKDGSRVRVIEASNDWPVAQVRRSLKEG"
BASE COUNT          573 a          741 c          736 g          555 t
ORIGIN      
        1 ttagctggcg tgcgatccat tctgccgtcg gatttgttgt gacgttgatc cagaccaggt
       61 ctcggcggcc caggcggacg atgacggcat agagcagatc gaagccaatg gtcgggacga
      121 tgaacaggtc catggcggca atgtccggcg cgtggttacg cagaaaggtg cgccatccct
      181 gacttggcgg cccacgccgc ttgaccatgt acttggcgac gctcgactgc gcgacttcaa
      241 acccaagctt gagcagttcg ccgtggatgc gcggcgctcc ccaaagcggg ctctccatgc
      301 tcatccgccg gatcagcgcc cgcaggtccg catcaatctg gggtcgccct ccccgcgggc
      361 gcgatttcca acgccagtaa cggcgaaagc cggcccgatg ccaccgcacg agcgtctcag
      421 gctgaacaat tgtgacgacc tgcaaaatcg acgggaacca tcggtacagc tggataagaa
      481 accagcggtc gttgttcctc agccggatcc ggccctgcac cttacgccgc agaacgatca
      541 actgatgtcg gagaaccgca ttctctgcct caagtcggct cttcgacttg aatggcgagg
      601 ccaagacggc caggacgaag cagaacagtc cgaacatggt ccactgaagt taggtgattc
      661 cttcggataa tcaatacgga taaggttttc ggtacacaca cctgatggca gatcgcacaa
      721 cgaggttaag tcaggttttg ggctgataac tagtctaacg ggatggacgt gggtggaaca
      781 ataccgcgac ctacctgtcg aaaaggatac gaagatttca aaagcgacaa tgcacaaatc
      841 ggtttatcaa agattcgatt taacgaaggc gtttgaatat gatgcctgga ttccttatcg
      901 accttcaaca ctcagcaatc atattgattt tgcgagatac tatgagccag gcgcagtctt
      961 tccggcaaca tcactaagta ccgcaacgga aatggtctat gatcctgaga cacgcgacca
     1021 atttggacca gcggtttcct aaaccgttgc agcccatctt ggcctgtatc atgcagttcc
     1081 gtttaatccc gaaattcgga cgtttttgac tcatcaccat ccatctaatc ccgccatttc
     1141 ccaccctgta gtgaggagtt cggtgggaaa tcgggtggga aaaatcggaa ctgtaaaacg
     1201 ctggatctga cccgactgcg caatgaccgg agggcagcgc gggcctattc gctttccatg
     1261 agcgctagct tcttctgaag ctcttcattt tttgttcggc cgttttggcc gtggtgaatt
     1321 tcgcgatgac aggtctgttt gaatgtcaga aagcgcggcg tgcgatagcc ggccgtttgc
     1381 gccatccaca aaagcacgac gttgcttcct gagtaaggcc gattggttcc ggcgttgcac
     1441 ggcgtattca tgccggccgt tgccgaccaa ggcttgatcc atggcgccgc cccacgctcc
     1501 aactccgcga caatgcgcgt cgatacctct gcatagagat tacgtttcat agcaccgccc
     1561 tcgctgttac agccacgcgc gaccgtcttt tgcgaacggc cgcacaccat tgccgcgtcg
     1621 agcagaattt ctaggttttg cagcgtgatg cgaggctgac ggtgtcaggg ggcggggcgg
     1681 gactcccggg gtacgctgga caggccaccc gtctcgcgcg tcgatagacg tggttgagtc
     1741 atacccaagg gtcgattttg gtcgttaagt ttttgttaag gtttgacggc cgagcacatg
     1801 cccgattcgc tctccctacc gcgctaacgc gctctgagcg gctgcgcgac cttcggcgtt
     1861 caagatgaga gatcgaaaga cctcatcggg aaggaactca ttcgatcggg taagcgtttc
     1921 attggtctcc ccaacaagcg ccaggaaacg tgatgctaat tcagaagcac gacgaaagtc
     1981 acattgtcga acttgaagac ggttctagat ggcggatttg gcccagcgat attgctgcga
     2041 ccttgcaatg gttgccgacc accgaactcc gggtatcagc aataagcgac gagttttgtt
     2101 cgcacaccct cattgatcaa aaagacggtt cacgggtgcg tgtgatcgaa gccagcaacg
     2161 actggcccgt tgcgcaggtc cggcgatcgc tgaaggaagg gtgaattaga gaagcaagcg
     2221 ccgtcggaca tgctctctgg cggagccact tccctacctg ggagcaacgc ggcaactggc
     2281 cgcctcgcac tcgccgttgg cgatacgcct ttacaattcc gccgcgacgt aattgtcgcg
     2341 gcggcgtcgg ccgaataaca aggaacggga tgccgtctcg gccgttgtct agtgccgccg
     2401 cgtgcgctcc gcaaagcgag gatgcaatgg cgccatcaaa tcatttgcct aaattgagga
     2461 tcgcgctgct gtttggggcc gcgctgatgc cggctctttc gtcagcgcag tccgttcaag
     2521 ctccgccatt gcccaaggcg ccgcccgaaa taatgtccaa ggaaacgcgc gacccgaaag
     2581 cctgcgcgca gcagggcctc gccgt
//
LOCUS       DATMGX010000189         4988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_271547, whole genome shotgun sequence.
ACCESSION   DATMGX010000189 DATMGX010000000
VERSION     DATMGX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271547"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VNR65_03050"
     CDS             complement(<1..306)
                     /locus_tag="VNR65_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17685.1"
                     /translation="MRLRDSAGRIPDFGEQAYAGCMIDEGAIQYRWNTVGSKLDERGR
                     RLFAAAEVRTAGWGGLSVVARITGLARSTINRGEDDLDAEPLTKGKIRRSGGGRRAVA
                     "
     gene            907..2007
                     /locus_tag="VNR65_03055"
     CDS             907..2007
                     /locus_tag="VNR65_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWJ17686.1"
                     /translation="MTTSTRSIWARAGAVLLAICLAQGAGHAQPASPPPVEKELVVAI
                     KEAPPFVMKHEDGTYYGIGIDLWRRVADRLQLRYGFLEQPTSEALLRGTAAGLYDAAF
                     GALDVTAARQRLVDFTQPFYATGLGIAVPVNESKLLTVSRILFSADFLRTVLLLIGIA
                     LGVGFLVWLFEHRKTNHFQGGKGLASGFWWSAVAMTQAGAALDAPATLPGRFVAICWM
                     IVSIIILTVFTAGITSTLTKQELRGTIHGVDDLRFVRVGDSRGTVTVSYLDRERISHR
                     NFPDPKDGLLALQAGKIDAFVYDRPLLNWMVMREFSETLRMLDFTVDTLNYAIALPKG
                     SRLADRLNIAVLEETESDWWQQTLFQYLRKKQ"
     gene            2121..2306
                     /locus_tag="VNR65_03060"
     CDS             2121..2306
                     /locus_tag="VNR65_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17687.1"
                     /translation="MRREHFVVHRSPVEAQNEIGLVEGHGAGRLGSARCTLTAASPEY
                     DRIGNHSCQDYETRGET"
     gene            complement(2432..3007)
                     /locus_tag="VNR65_03065"
     CDS             complement(2432..3007)
                     /locus_tag="VNR65_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17688.1"
                     /translation="MPELPSAADHGDIDQKRRHSRFLSFLPYLLILIFTLVGVAYTSI
                     NKKPLILYWEILAVFTGVLCVVGGWRHTQSRADRVRLIWTQALHWAAFLATMNLLLIS
                     DVQRMLNADATGLAVLLLLALGTFVAGVHSLSWQTCTLGVVMALAVPAIAWIEESALV
                     LLLSAIALIGLSSLFWWFGSRRTPAVELEGA"
     gene            complement(3351..>4988)
                     /locus_tag="VNR65_03070"
     CDS             complement(3351..>4988)
                     /locus_tag="VNR65_03070"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HWJ17689.1"
                     /translation="QAWEVAHACAGDVPRLSRGVLPGEGRIKACMKEKMAQLSPAYRD
                     VLAVAVAGENAPSEEASMKKTAPTRTAYKYATPMPPGVVAPDTLETRFGTLNFFGGFP
                     DKASVDKLYDNLDFQRAVQGYLLALPVVNQVANRDAILAFGPANTTVPIWENLVDSRT
                     VGLTFNDNTPYTWMWVDLHGGPLVIEVPPKVLGLVDDIWYKWNGDIGITGPDKGKGGK
                     YLLLPPGYEGEVPKGYFIIRPGSYWAWPVWRSFLVDGDPKPGVDLVKKSLKIYRLADA
                     ANPPKLNFVNISGKPFSMDAPADYRFWELLNKVVQDEPAYAVDPTTLGFWASVGIMKG
                     KPFAPDERMKKILTEAAAVGDATARAIMYRWRGANGYWYPNSNWRFGFVGGYKFEENG
                     ARLLNAYSGFFFYATGITPAMDTQIVGEGSQYMVGFVDSKGDVLDGGKSYKLHLPRNI
                     PVKDFWSVIVYDNQTRSMLQTDQQYPSVSSQTKGLKVNADGSVDVYFGPKSPAGEEKN
                     WVQTVPGKSWNTLLRLYGPLQPFFDKTWRPGEIELQP"
BASE COUNT         1033 a         1524 c         1422 g         1009 t
ORIGIN      
        1 ggcaacggcc cggcgcccgc caccagagcg gcgaatcttg cccttcgtca gcggctccgc
       61 gtcgagatcg tcctcaccgc gattgatcgt cgagcgggcg agccccgtga tccgcgccac
      121 aaccgagaga ccgccccatc ctgcggtccg cacctcggca gctgcgaaca atctccggcc
      181 tcgctcgtcg agcttgctgc cgaccgtgtt ccagcgatat tgaatggcgc cctcgtcgat
      241 catgcaaccg gcatacgcct gttcgccgaa atccggaatc ctgccagccg agtcacgcag
      301 acgcacgccc acagagcgcg attcacttat tgttcgggat gtccttagca cgaatgctgg
      361 gataggaggg ctactgcagc tgagtgctgc gcattgaaaa cgcgtgcttc aggccgggag
      421 cagactaggc gacatcgaaa acttagttag atcgcctcgc ccgctttctg atcgcttaac
      481 ggactaccat ggcttgagcc tcagacggtg aaggtgggcc aaaaccgcgc ctcgcggccg
      541 cccttgaaaa ggggcttaat ttttctcggt tcggccccct gttgccgaaa aaggcatcgc
      601 gtatggagcg cttcagcagg gccgcctcct tatccagcct gcggacagag gggtcaggcc
      661 ggagccacgt ggctcaatac ctgcacaata aaggcaatag tagatgtgct tcgaaaaact
      721 tgcgcgtggc ggtccaccac ggcattaaga gtgatgggga atcttggtcc ttccaggaat
      781 cccttgcaca ctgcgtgacc ccccgtcagg ggcatccatt ccaagccaac caaaaccgtg
      841 caggcacgcg ctgatgccaa tgaaccctgt ccatcgattg cgcgcactat ttcggggcgg
      901 ccgcggatga cgacgtcaac gcggtcaatc tgggcgcggg ctggggctgt gcttctggcg
      961 atctgccttg ctcaaggcgc aggccatgcc cagcccgcaa gcccgccgcc cgttgaaaaa
     1021 gagctggttg tcgccatcaa ggaagcgccg ccctttgtga tgaagcacga agacgggacc
     1081 tactacggca tcggcatcga tctttggcgc cgagtcgcgg atcgtttgca gcttcgctac
     1141 ggttttttgg agcaaccgac atcagaggcg ctgctaaggg gaacagcggc aggcttgtac
     1201 gacgctgcct ttggagcgct tgacgtcaca gcagcgcgac agcggcttgt cgacttcacc
     1261 caaccctttt acgcaaccgg cctcggcatt gccgtgccgg taaacgagag caaattgctc
     1321 accgtttcgc ggatcctttt ctcggctgac tttctccgga ctgtcttgct cctgattggc
     1381 atcgcgctcg gcgtcggctt cctggtctgg ctcttcgagc atcgcaaaac caatcatttc
     1441 cagggaggta agggcctcgc ctccggcttt tggtggtctg ccgtggcgat gacgcaagcg
     1501 ggagccgcgc tcgacgcgcc ggcgacgctc cccggccgtt tcgtggccat ttgctggatg
     1561 atcgtatcca tcattatcct tacagtcttc acggccggca taacctcgac actgaccaag
     1621 caggagctgc gggggaccat ccacggcgta gacgatcttc gcttcgttcg cgtcggcgat
     1681 tcccggggta cggtaacggt cagctatctc gatcgcgaac ggatttccca tcgcaatttt
     1741 cccgatccga aggatgggct cctggcgctg caggcgggca agatcgatgc cttcgtctac
     1801 gataggcccc ttctcaactg gatggttatg cgagagtttt cggagacgct gcgcatgctc
     1861 gactttacgg tcgacactct gaattacgcg atcgcgctcc ccaaaggcag ccgcctcgcg
     1921 gataggctga acatcgcggt tttggaggaa accgaaagcg attggtggca gcaaacgctg
     1981 ttccaatacc tccgcaaaaa acaatgagag tcgaggtgtt gcctaaagct tgatgtgttg
     2041 caaaagcaca tccttggcct cgttgaggcc agatagcatg tgccgccctg atcggggtgg
     2101 aaccgcttca tgagattgcg atgcgccgcg agcatttcgt cgtgcatcgc tccccggttg
     2161 aggcccaaaa cgagataggc ctcgtcgagg gtcatggcgc gggaagactt ggctccgcgc
     2221 gttgcactct caccgccgcc tctccggaat atgaccgcat aggcaatcat tcctgccaag
     2281 actacgaaac ccgcggtgag acctgacttg atgcttttac ccttcatggc gaactccatc
     2341 agcaaagcaa gacggcgacg ttgttcaatg cgcatgcaat cagagtattg ctgcaggctg
     2401 ttattttgcc gccgcgccct cgcaggatgg attacgcgcc ctccagctca accgccggcg
     2461 tccggcggct cccgaaccac cagaaaaggc tactaaggcc aatgagggcg atcgcagaaa
     2521 gaagaaggac cagcgccgat tcctctatcc aggcgatcgc cggaaccgca agcgccatca
     2581 cgactccgag ggtgcatgtc tgccaggaca agctgtgaac gccggcgaca aaggtgccaa
     2641 gggcaaggag aagaagcacc gcaagtccgg tcgcatcggc gttcaacatt cgctgaacgt
     2701 ccgagatgag caggagattc atggtcgcca ggaaagcagc ccagtgaaga gcctgcgtcc
     2761 agatgagcct gacgcgatcg gctctgcttt gggtgtgccg ccaaccccca acgacacaca
     2821 ggacccccgt gaaaacggcg agtatctccc aataaaggat gagcggcttc ttgttgatgc
     2881 tcgtgtaagc cacgccaaca agggtgaaaa tcagaatgag cagatagggc agaaaactga
     2941 gaaaacgcga atgccttctc ttttgatcga tgtctccatg gtcggccgct gatgggagct
     3001 cgggcatcga aatgcactcc tttctaaaga atgaagtcac ccccgtactt acttataatg
     3061 cgctcccgcg gcgtgaaccg gaggcgagtt cttccctatc cctgccgcga aggcgagcgc
     3121 gttctcgcga taccgtgatc gtcttcatgg gctccttccg agtcgtggtc gcctaatcgg
     3181 caaccatttc tttggcgctt acgcgcccgt gaaggagccg tccacagcat caacaacaga
     3241 cccctctctg ataaaatgcg gcgccgacca gggatgctgg actataacac cgcagggaac
     3301 gacgattgcg ccaggcggct gctcgctcac tgccgcccag caaaggccgg tcagggctgc
     3361 agctcgattt cgcccggccg ccaggtcttg tcaaagaaag gctgaagcgg cccatacaac
     3421 cgcaacaaag tattccagct ctttcccggg actgtctgaa cccaattttt ttcctcgcca
     3481 gcgggggatt tcgggccgaa atacacgtcg actgagccgt cagcgttaac cttcagccct
     3541 ttagtctggc tgctgacgct gggatactgc tggtccgttt ggagcatcga ccgcgtctgg
     3601 ttgtcgtaga cgatcaccga ccagaagtcc ttgactggga tgttccgtgg caaatgcagc
     3661 ttatagctct tgccgccgtc gaggacatcg cccttcgagt caacaaaccc taccatgtac
     3721 tgcgatcctt cgccgacgat ctgggtatcc atcgccggtg tgatgccggt cgcatagaag
     3781 aagaagccgg aatatgcgtt cagtaaacgt gcgccgtttt cctcgaactt gtagccgcca
     3841 acaaacccga accgccaatt gctgttcggg taccaatagc cgttagcccc acgccatcta
     3901 tacatgatgg cgcgggcggt cgcgtcgccc acggccgctg cttcggtcag aattttcttc
     3961 atgcgctcgt cgggtgcgaa aggcttgccc ttcataattc cgaccgaggc ccagaagccc
     4021 agcgttgtcg ggtcgaccgc ataggccggc tcgtcctgca cgactttgtt cagcagctcc
     4081 cagaagcgat agtcggctgg cgcatccatg ctgaatggct tgccggatat gttgacgaaa
     4141 ttgagtttcg gcggattggc cgcgtcggcg agccgataaa tcttcaggct ctttttgacg
     4201 agatcaacgc cgggcttggg gtcgccgtcg acgaggaagc tacgccaaac cggccaagcc
     4261 caatatgaac cgggtcgaat gatgaaatag cctttcggaa cttcgccctc atagccgggc
     4321 ggcagcagca ggtattttcc gcctttaccc ttatccggcc cggtgatccc gatgtcacca
     4381 ttccacttgt accagatgtc gtcgactagt ccgagcacct tcggtggcac ctcaataacc
     4441 agtgggccgc cgtgcagatc gacccacatc caagtgtacg gcgtgttgtc gttgaacgtg
     4501 agcccgaccg tgcgcgagtc gaccaagttc tcccagatcg gcacagtcgt attggccgga
     4561 ccgaaagcga ggatggcatc gcggttggcg acttggttca ccacgggcaa cgctagcagg
     4621 tacccctgca cggcccgctg aaagtcaagg ttgtcgtaga gcttgtcgac gctcgcctta
     4681 tcgggaaagc cgccaaagaa attcagcgtg ccgaaacgag tttccaaagt gtcgggcgca
     4741 acgacgccag gtggcatcgg tgtcgcatac ttatacgcag ttctcgtggg tgcagttttc
     4801 ttcatggacg cctcctcgga cggcgcgttc tcccccgcaa cagcgacagc aagcacgtcg
     4861 cgataggcgg gggacaactg agccatcttc tccttcatgc acgccttgat ccggccttcg
     4921 ccaggaagaa ccccgcggga gagccgcggg acgtcgcctg cgcaagcatg cgcaacctcc
     4981 cacgcttg
//
LOCUS       DATMGX010000190         3201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_146441, whole genome shotgun sequence.
ACCESSION   DATMGX010000190 DATMGX010000000
VERSION     DATMGX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146441"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VNR65_03075"
     CDS             <1..864
                     /locus_tag="VNR65_03075"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWJ17690.1"
                     /translation="NASGALSGNSVLYVIKPNGKLVRQVAIANSSPHTLGLAFNPING
                     FLLVLDFGAGKVLHVDPTTGLSSVFAVPTLPNPGLNALTFDKQGNVYISDSFNGVIWK
                     TGPLGGAPTIWSNDPLLGPGTGLTPPFGANGVEFNNAGTTMFVANTATHQIIQIPLNM
                     NGTAGTASIFITGINAPDGIAIDRKDNLWVCANQEDDIIVIDKTGKVIAKLGDFNGID
                     DDGVVRGLLFPASPAFSRDGKTLYVSNLTLFLPYAGANLAVDSAWTLKVKHYTVSKMR
                     AVIPSLRDDHD"
     gene            complement(1000..1290)
                     /locus_tag="VNR65_03080"
     CDS             complement(1000..1290)
                     /locus_tag="VNR65_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17691.1"
                     /translation="MFVRQEGNLIAIHVDPSDPTAWRREPFFQHIKAFATKAADTKQQ
                     VAVYIKNRVIVIFPNKEVDVGTMNPGDHLVVRELVGQRGRDWQAFIEIGAGP"
     gene            complement(1907..>3201)
                     /locus_tag="VNR65_03085"
     CDS             complement(1907..>3201)
                     /locus_tag="VNR65_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17692.1"
                     /translation="GAPTTTHTKCITAVQIPGKPIRSFDISWVNPDRAEYYLGDRSNA
                     GIDIINTQTLTFKRTIGGFVGVVLNTAGTAVNNAKSGPDGVTSHGRWLYAGDGDSTLK
                     VIDLNAPTASAIKQSISTGGTTRLDEMALTTDGKLLIAANNAEDPPFATLFNANGDAA
                     TSNVTKITKISVDATIVPAGAGLSLEQPTWDPKTKRFYTSIPIIKDNPAGCEYGQSTP
                     AKPITCDGGMLVTDPFALSTPTATQGAFNATTNTGVVPLHACGPNGATVGPNDNLMLG
                     CTPGNNPSNTTTLVINATTKYYANIGGITGSDEVWYNKGDNRYYLGASKMIGGAELGV
                     VDGTSVLIEAIPQSANSHSVAADSKRNRIFVPQVAPTSVVGAGGDSTTVGQGICGSMS
                     GCVAVYQHTVNDDDDDHDHADDNDRDRRDRDDKEHGRK"
BASE COUNT          654 a          910 c          908 g          729 t
ORIGIN      
        1 aacgcgagcg gcgcgctcag tgggaactcc gtgctgtacg tcatcaagcc gaatggcaaa
       61 ctggtccggc aggtggctat cgccaattcc agtccccata cgctcgggtt ggcctttaat
      121 ccgatcaacg gatttctgct ggtcctcgat ttcggagccg gcaaagtcct acacgttgat
      181 ccgacgaccg gcctttcgtc agtctttgct gtccccacat tgccgaatcc tggcctcaat
      241 gcgctgacct ttgacaagca aggaaacgtg tacatatcgg attccttcaa cggcgttatc
      301 tggaaaaccg gcccccttgg cggcgcgccg acaatctgga gcaatgaccc attgctcgga
      361 ccgggaaccg gcctcacgcc gccattcggc gcgaatggcg ttgagttcaa caatgccggt
      421 accacgatgt ttgtcgcaaa caccgctacg catcagatta tccaaatccc gttgaacatg
      481 aatgggacgg cgggcactgc ttcgattttt atcacgggga tcaacgcccc cgatgggatt
      541 gcgatcgatc gcaaagacaa tctctgggtc tgtgccaatc aagaggacga catcatcgtc
      601 attgacaaaa ccggaaaggt gatcgcgaag ctgggcgatt tcaatggaat agatgacgat
      661 ggcgtcgtgc gcggtctgct ctttccggcg agcccagcct tcagccggga tggcaagaca
      721 ctctacgttt cgaacctgac cttgttcctg ccttacgctg gcgcaaacct ggcggtggat
      781 tcggcatgga cgctcaaggt caagcactac acggtatcca agatgcgcgc tgtgattccg
      841 tcactcagag acgaccacga ttagtccagt ggcgcaacaa cagcgcccgg ttgctcacgg
      901 cagcgttaaa tcgctctcgt cacaacgcta cgggatgcct ttgctgcgcc gcttcaatct
      961 gaattcagaa caagcatcga aattcgggat ataatacagc taggggcccg cgccaatctc
     1021 tataaaggcc tgccaatctc tgccgcgctg gcccaccagc tccctgacca caaggtggtc
     1081 gccgggattc atagtaccca cgtcaacttc cttgttcgga aagatgacga tgactcgatt
     1141 ttttatgtag acggccacct gctgcttggt atcggcggcc tttgttgcga atgctttgat
     1201 atgttgaaaa aacggttcgc gacgccaggc cgtggggtcg ctcggatcga cgtgaatcgc
     1261 gatcaagttg ccttcctggc gcacgaacat cttgcacttg ttcgggcgcc actcgggacc
     1321 tagagccgcc gatatgagcc agtagcaata aaagtcgcga cactcggttg gccgggtttc
     1381 atagatagtg catccgccgc ggccaggggc gcagtgagcg caccatgtcc cagccggctt
     1441 gttgaacgtt tcaatccggg ggagtttgca gcacagcgag cattcgccgc aacttcgccc
     1501 aggcaggagt cgaaccggaa ccggatctct cgcatcgctt ggcacctggc tcattaccgc
     1561 gcgcctgtcg aattgaattg cccaggttcg ttccatcact ggtatgaaaa tcgcctgcct
     1621 ggctcgtgcc gccattggaa ttggcgactt cccgaaatag acctgaacca cggctcttgg
     1681 gtcaagagcg cgataagggg tcaaaaccgc gatgcggtaa tcgtccgcag tgggagccga
     1741 ttatcctccg gggttcttca cctctctgac atccaggtgc atttctgcac acaaataccc
     1801 aaaaggccgg ggcaatagcc ccggcctcct ggtccgcaac tcaattctgg ggcactgtcg
     1861 acttcacaat cgtcaatggc ctagacctgt cacaagtgct gtgacgttat ttacggccgt
     1921 gttccttgtc gtcacgatca cggcgatcgc gatcgttgtc gtctgcgtga tcatgatcgt
     1981 catcgtcgtc gttgacagta tgttgataga ccgcaacgca tccgctcata ctgccacaga
     2041 ttccttggcc aacggtcgta gagtcgccac cggctccaac caccgaagtc ggcgcaacct
     2101 gaggtacgaa aatgcggttg cgcttggagt cggccgcaac tgagtgcgag ttagcagatt
     2161 gcgggatggc ttcgatcagc acacttgttc cgtcgacgac gccgagttca gcgcccccaa
     2221 tcatcttgct ggcgcccaga tagtagcgat tgtcaccctt gttgtaccag acttcatccg
     2281 agccagtgat gccgccaatg ttggcatagt acttcgtcgt cgcattgatg accaacgtgg
     2341 tggtgttgct tgggttgttg cccggggtgc agcccaacat gagattgtca ttcggcccaa
     2401 cggtggcacc attgggtccg caggcgtgaa gcgggacgac cccggtgtta gtggtcgcat
     2461 tgaaggcgcc ctgcgttgcc gttggcgtcg agagagcgaa tgggtcggtc acgagcatgc
     2521 cgccatcaca ggtaatcggc ttggctggag tcgactgccc gtattcacat cctgccgggt
     2581 tatctttgat gatcgggata gatgtgtaga accgtttggt tttgggatcc caggtcggct
     2641 gctcgagcga taggccggcg ccagccggaa cgatcgtagc atcgaccgag atcttggtga
     2701 ttttagtgac attgctcgtg gcggcatcgc catttgcgtt aaacagcgtc gcaaacggcg
     2761 gatcctcagc attgttggcg gcgatcagca gtttgccatc ggtcgtcaac gccatttcat
     2821 cgagccgagt tgttccgccg gtgctgattg attgtttgat ggcacttgcc gtcggcgcat
     2881 tgagatcgat caccttgagc gtgctatcgc catcaccggc atagagccag cggccatgag
     2941 acgtaacgcc atccggcccg gatttggcgt tgttgacagc ggtgccggcg gtgttgagca
     3001 caacgccgac aaagcccccg atggtccgct tgaacgtcag ggtttgggtg ttgattatat
     3061 caatcccggc gtttgaacga tcgcccagat aatattccgc gcgatccgga ttgacccagc
     3121 tgatgtcgaa agaccggatc ggtttgccgg ggatttgaac ggccgtgatg cattttgtat
     3181 gcgtggtcgt cggcgctcct g
//
LOCUS       DATMGX010000191         3617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_14428, whole genome shotgun sequence.
ACCESSION   DATMGX010000191 DATMGX010000000
VERSION     DATMGX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14428"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1173
                     /locus_tag="VNR65_03090"
     CDS             433..1173
                     /locus_tag="VNR65_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWJ17693.1"
                     /translation="MPQEEVTAGLLVIGDEILSGRTKDRNIGFVADHCNQIGIRLKEI
                     RVVADDEAAIICAVNELRRAYTYVFTTGGIGPTHDDITADSISRALGVSIDVDPRAVD
                     LLRERYAEADLTPVRLRMARIPEGAELIENCVSKAPGFKIGNVIVMAGVPAIMEAMLL
                     AVTPRLKTGPKLLSDSIRVNAPEGHIAAQLAACQRTFPDVAMGSYPFFEDGIHGTNLV
                     LHSTEAGRLRLAKSDLISRLGPDLTVSE"
     gene            complement(1157..1399)
                     /locus_tag="VNR65_03095"
     CDS             complement(1157..1399)
                     /locus_tag="VNR65_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWJ17694.1"
                     /translation="MADETMQSVTIDTRGLSCPLPVLKVRKAMRSIENGTEVWVLATD
                     PLADQDLRAYCEASGCAFLSVDKLDGDVLCIVIQKR"
     gene            complement(1402..2091)
                     /locus_tag="VNR65_03100"
     CDS             complement(1402..2091)
                     /locus_tag="VNR65_03100"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWJ17695.1"
                     /translation="MNLFDSMATLDPAIIKAYVLHHKAWAGPILGVIAFGESLVLIGL
                     FFPATLIMVFAGLAIQQGKLDAASVIGWSIAGAVLGDIVTYWIGRWIGPSVVHHWPLN
                     RDKAAVARARLFFRKFGFLSIFIGRFLGPVRPTIPLVAGIMRMRHLTFQIANVTSAIV
                     WVPFMLFWGYAFSKAVGDLSHMTTGNWVALIVFLVAVMMTLTFATVRMQGKSAKDKRQ
                     EKRSAAVKLAE"
     gene            complement(2321..3049)
                     /gene="recO"
                     /locus_tag="VNR65_03105"
     CDS             complement(2321..3049)
                     /gene="recO"
                     /locus_tag="VNR65_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWJ17696.1"
                     /translation="MRWADDGIFLLGRPHGESSVIADIFTRKNGRTRGLVKGGRSRRI
                     RPILQTGNGLKVEWRARLDDQLGVYTAELTEATASRVLDDKLSLAGVTALAGLLQILP
                     ERDPHPGLFDAARACLDSAGETCFPVSLIWFELRFLNELGFGLNLTTCVATGRTDELA
                     YVSPKSGQAVSRQAGEPYRDRLLALPAFLSRAQGADQPCHADLRSAFAMTGHFLGAHV
                     FAGTGKPIPKAREEFTRLLHQRGE"
     gene            complement(3075..>3617)
                     /gene="era"
                     /locus_tag="VNR65_03110"
     CDS             complement(3075..>3617)
                     /gene="era"
                     /locus_tag="VNR65_03110"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWJ17697.1"
                     /translation="AINKVDKIHKEKLLSLAEGASKRLQLDRLFMISSLSGDGVEDLR
                     EYLAGRMPPGPWLFPEDQIADAPLRQWAAEVTREKMFLRLHEEIPYSSTIETTDWKQL
                     KNGSIRIDQTIFVERESQRKIVLGSKGQAIKQISMEARREIAEGIETQVHLFLFVKVR
                     ENWADDPERYREMGLEFPKS"
BASE COUNT          828 a         1052 c          985 g          752 t
ORIGIN      
        1 atagatagag ccagtccccc acttgaaata tggcagggat aggtcaaagt ctcaactccc
       61 gaagccgttg gggccggaaa aagcttgcaa tggggcgcct cagtctccgg atagccacct
      121 cgcaggattc attgccgaag catcacggag aaggacgatg cggctacttg caccacgtcg
      181 gactcaattt ataaatattc tgcggaaaag aaattctcct atgccgatta agaatatatc
      241 tagcagttat ccaatccatt gcgactttta aaccctgtca ccctatcaag gttgataggt
      301 gctaagcgaa gttaggatcg tttctactga ctcacaatct tgtgagcatc gttgtgagag
      361 gtgcccgctt tcgagaccac gaaagataaa gaaatgaacg ttaccgtttt atcttgtgtg
      421 caaaggcgtg caatgcccca agaagaggtt accgcagggc tcctcgttat aggagatgag
      481 attctttcgg ggaggacgaa ggacaggaac atcggcttcg tcgctgacca ctgcaaccag
      541 atcgggattc gcctgaaaga gatccgcgtt gtggccgacg atgaggctgc gattatttgt
      601 gcggtcaacg aattgaggcg agcctacact tacgtcttca caacgggcgg catcggcccc
      661 actcacgacg atattactgc cgactcgatt tcgcgcgcgc tcggggtatc aatcgatgtc
      721 gacccgagag ctgtcgattt gctccgcgag cgctacgccg aagccgactt gaccccggtg
      781 cggctgcgca tggctcgcat ccccgaaggg gcggaactca tcgaaaactg cgtttcgaaa
      841 gctcccggct tcaaaatcgg caatgtcatc gttatggcag gcgtacccgc catcatggag
      901 gcgatgcttc ttgctgtcac gccaaggctg aagaccgggc caaagctgct gtcggattcg
      961 attcgcgtta acgccccgga aggccacatc gccgctcagc tagccgcttg tcagcggacc
     1021 tttcctgatg tggcgatggg cagctatccc ttttttgaag atggaatcca cggcacaaat
     1081 ctcgttttgc actccacaga ggcagggcgc ctgaggcttg cgaagtccga cctcatcagc
     1141 cgattgggac cggatctcac cgtttctgaa tgacgatgca aagcacgtcg ccgtcaagct
     1201 tatcgacgga gagaaatgcg catcccgacg cctcgcaata agctcgcaga tcctggtccg
     1261 caagcgggtc ggtagcgaga acccaaactt ccgttccgtt ctctatggag cgcatggcct
     1321 tgcgcacctt caacacagga agcggacaag atagaccgcg cgtgtcgatc gtgacgctct
     1381 gcatggtctc atcggccaac gttattccgc aagcttcaca gcagcggacc gcttctcctg
     1441 ccgcttatcc ttcgcgctct ttccctgcat cctaacggtg gcaaaggtca gcgtcatcat
     1501 caccgctacg agaaacacaa tgagggctac ccaattgccc gtggtcatat ggctgagatc
     1561 gccgacggct ttcgaaaagg cgtagcccca gaatagcatg aagggaaccc agacgatcgc
     1621 cgaggtgacg tttgcaatct gaaaagtcag atgacgcatc cgcatgattc cggctacgag
     1681 agggatcgtc ggccggaccg gcccaagaaa acggccaata aatatcgaaa ggaagccgaa
     1741 tttgcggaaa aatagccggg cgcgggcgac tgcggcctta tcacggttga ggggccaatg
     1801 atgcaccacg ctcggcccga tccagcgccc gatccagtat gtgacaatat cgccgagaac
     1861 agctccggcg atcgaccacc cgatgaccga cgccgcatcg agcttgcctt gctgaatggc
     1921 gagcccagcg aaaaccatga tcagggtggc ggggaaaaaa agcccaatga ggacgaggga
     1981 ctcgccgaac gcaatcacac cgaggatggg gccagcccat gctttgtgat ggagtacata
     2041 cgcctttatg attgccgggt cgagcgtggc catggagtcg aatagattca cagaattccc
     2101 ttttcctacc cggatggcaa gccgcagcta ttatgcgtcg cgcaactgtt cgcgcagtgc
     2161 gttcagctcg ctatccccca caacgagttc ggcgctcccc tatcatgaga gctcatctcg
     2221 caatccccaa aacggcgcta gcaccaatag agtaccacgg caacacctaa gtttttgctc
     2281 ccaaaagcca cgcttaaact tctgactgtc aaattttgcc tcattcgccg cgctggtgca
     2341 aaagccttgt gaactcctcc ctcgccttgg ggatcggctt cccggtcccc gcgaagacat
     2401 gcgcgccgag gaaatgcccc gtcattgcga atgccgatcg caaatctgca tgacatggct
     2461 gatctgctcc ctgcgcgcgg ctcagaaagg ccggcaaagc gagcaacctg tcccggtaag
     2521 gctctcccgc ttgtcggctc acagcttgac cagattttgg cgagacatag gcaagttcgt
     2581 ccgttcggcc agtcgctacg caggtggtga ggttcaatcc aaacccaagt tcgttgagaa
     2641 atcgaagctc gaaccagatc agggacaccg gaaagcatgt ctcgccggcg gaatcgagac
     2701 atgctcgggc ggcatcgaaa agccccggat gaggatcgcg ctccggcaaa atctgcagaa
     2761 gcccggcgag tgccgttacg ccggcaagcg agagcttatc gtcgagaacg cgcgacgcgg
     2821 ttgcttccgt caactcggcg gtatagacac cgagctgatc gtcgaggcgc gcgcgccatt
     2881 ccaccttgag gccatttcca gtttgaagaa ttggccggat tcgccgggag cgcccgccct
     2941 tcacaaggcc gcgggtacgc ccgttcttgc gtgtgaagat atcagcgatc acgctcgatt
     3001 cgccatgcgg ccgacccagc aggaaaatgc cgtcgtcagc ccatctcatg aagcattgtc
     3061 cgcaacgagc gtattcatga cttcggaaac tcgagcccca tctcccggta gcgttcggga
     3121 tcgtcagccc aattctccct taccttcaca aaaaggaaca gatgaacctg cgtttctatg
     3181 ccctcggcaa tctcgcgccg cgcttccatc gagatttgct tgatggcctg cccttttgag
     3241 ccgagcacga ttttgcgctg actttcgcgt tccacaaaga tcgtttgatc gatgcggatc
     3301 gagccatttt tcagctgttt ccaatctgtc gtttcgattg tcgacgaata ggggatctcc
     3361 tcatggagcc gcaagaacat cttctcgcgg gtcacctcag cggcccactg acgaagtgga
     3421 gcgtcggcga tctgatcctc ggggaaaagc caggggccgg gcggcatgcg gccagcgaga
     3481 tactccctca ggtcctcgac gccgtcgccc gatagcgacg agatcatgaa aagcctatcg
     3541 agttgaagcc gtttcgaggc cccttcggcg agcgatagca gtttctcttt atgaattttg
     3601 tcgactttgt tgatggc
//
LOCUS       DATMGX010000192         2870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_7365,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000192 DATMGX010000000
VERSION     DATMGX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7365"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VNR65_03115"
     CDS             complement(<1..567)
                     /locus_tag="VNR65_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ17698.1"
                     /translation="MDDQVMAVGANMAGTGFKAAGCGSTFNRIQRRDFLALLGGAATW
                     ASPSHAQKRAVPVIGYLCPESPGLFASRLKAFHEGLGESGYVEGRNVAIDYQWAEGQY
                     NRLPALAANLVARNVDVIVAPGGAPVALAAKASTASKTIVFEMGGDPVQLGVVDSLSR
                     PGGNITGVSSLSVDVSPKRLEMMHELLPA"
     gene            complement(649..2388)
                     /locus_tag="VNR65_03120"
     CDS             complement(649..2388)
                     /locus_tag="VNR65_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWJ17699.1"
                     /translation="MMVRQRFRGLFQKYLLVLFMAVAIPLAINGVIEAWLGYRDQRAR
                     LDQLLGIQATSAAARIHGFIDGITSELGWLVQLPWTDDPDERRRTDALRLFRQAPAIV
                     SLALLDQRGRERLYVSRIGLNRIDSRTDRSADPAVVGARSARIWFGDVSYNRGSEPYL
                     IVAISGNRASGGTLIAEVNLKLIWDVISAIKVGKTGFAFVLDRPGRLIAHPDISLVLR
                     GAEEATSKPFQAIRDAISRAGVGFATGQDVQGQEVAAAAAPVAGPDWTVVVEQPLSEA
                     FGPIYAALWRTAGLLLVGTIFSGLLAYALADRMTEPIRLLEEGTERIGAGLFDHRITI
                     ETGDEFQRLADSFNRMAAELALAQEQQERIARLKRFLAPQVADLVDRTGDDSVLEGRR
                     TEVVVVFCDLRGFTSFSAEVAPEEVMSVLSEYYEALGKAITTFAATLTSFTGDGLMVL
                     VNAPVPVEEPALRAVDLAVEMQRTVQELIVRWRARGDHIGFGIGLASGSATVGRIGYE
                     SRFDYTAIGSVVNLAARLCASAADREILMNAEVAEAVKDRRSLKGLGNRPIRGYDQAM
                     PVFGISFDAPSKT"
BASE COUNT          615 a          876 c          809 g          570 t
ORIGIN      
        1 cgctggcagc agttcgtgca tcatctcgag ccgcttcggc gacacgtcca cgctcaagct
       61 tgaaactccc gtgatattgc cacccggccg cgacaagctg tccaccacgc ctagttggac
      121 tgggtcacca cccatctcaa atacgatcgt cttactcgcc gtagaagctt tcgccgcgag
      181 cgccacggga gcgccgccgg gcgcgacgat gacgtccaca ttacgggcga ccagatttgc
      241 agccaacgcc ggtaatcggt tgtattgtcc ttccgcccat tgataatcga ttgcgacatt
      301 gcggccttcg acatagccac tttcgccgag tccttcgtgg aaagctttga gccgacttgc
      361 gaatagccca ggcgactccg ggcaaaggta gccgatgacc ggcacggccc gtttttgcgc
      421 atgtgacgga gacgcccaag ttgcggcacc acccagcagc gcaagaaaat ctcgccgctg
      481 aatccgattg aacgtactgc cgcaccctgc tgccttgaat ccagtgccgg ccatgtttgc
      541 accaaccgcc atcacttggt cgtccataag tgcggatccc gtgatgttca catagacggt
      601 gaatgggtct tgcattagac gtcgggtgcc aggccattta ccgtcggctt aggtcttgga
      661 cggcgcatcg aacgagattc caaacaccgg catggcctga tcataacccc tgatcggacg
      721 gttcccgagt cctttcagcg agcgtctgtc cttgacggct tcggcgactt cggcattcat
      781 caggatttcc cggtcggctg cagatgcgca caggcgggca gcgagattga ccacactgcc
      841 aatggccgtg tagtcgaacc ggctttcgta cccgatccgt ccaaccgttg ccgagccact
      901 agcaagacct atcccgaacc cgatatggtc ccctcgcgct cgccagcgaa cgatgagttc
      961 ttgcacggtc ctttgcattt caacagctag gtcgacggcc cttagcgcgg gttcttcgac
     1021 gggaacgggt gcattgacga gaaccatcaa gccatcgccc gtgaaactgg tgagtgtcgc
     1081 ggcaaatgtc gtgatggcct tgccgagcgc ctcatagtac tccgagagca cgctcatgac
     1141 ttcctctggc gcgacttccg ctgaaaatga ggtgaagcct cgcaagtcac agaacacaac
     1201 gaccacctcg gtgcggcggc cctccaacac gctgtcgtcg cccgttcgat cgacaagatc
     1261 ggcgacctgc ggggcgagga accgcttgag tctcgcaata cgctcctgct gctcctgcgc
     1321 gagagccaac tcggcagcca tcctgttaaa actgtcggcc agacgctgaa actcatcccc
     1381 tgtctcgatg gtgatgcggt gatcgaaaag tccagcgccg atccgctcgg tcccctcctc
     1441 gagaagccgg atcggctcgg tcatccgatc cgccagcgca taggcgagca ggcctgagaa
     1501 gatggtgccg accaggagaa gccctgcggt ccgccacaag gctgcataga taggcccgaa
     1561 agcttcggac aggggctgtt cgaccacgac agtccagtca ggacccgcaa cgggagcagc
     1621 ggcggcggca acctcttggc cctgaacgtc ctggcctgtc gcgaaaccga cccccgcgcg
     1681 gctgatcgca tcgcgtatgg cttggaaggg cttggacgtc gcttcctccg ccccgcgtag
     1741 cacgaggctg atatccggat gggcgatgag ccgacccgga cggtccagca cgaaggcgaa
     1801 gccggttttt cccactttga tggccgatat tacgtcccaa atgagtttga ggttgacttc
     1861 ggcgatcagg gtgccacccg acgcccggtt gccggaaatt gcaacgatca ggtatggctc
     1921 ggaccctcgg ttatagctca cgtcgccaaa ccagatccgg gccgagcggg ccccaactac
     1981 cgcaggatcg gcggatcggt cagtgcggct gtctattcga ttgaggccga tacgcgagac
     2041 ataaaggcgt tctcgccccc tttggtcgag gagtgcgaga ctcacgatgg caggggcttg
     2101 gcgaaacaag cgcaatgcgt cagtccgccg ccgctcgtcg gggtcgtcag tccatggaag
     2161 ctggaccaac cagccaagct cgctcgtgat gccgtcaatg aagccatgga tcctggctgc
     2221 ggcggatgtc gcctgaatgc cgagcagttg atcgagtctc gcacgctgat cgcgatagcc
     2281 gagccaggct tcgatgacac cgttgatcgc aagtggaatc gccacggcca taaaaagcac
     2341 gagcaggtac ttctggaaaa ggccgcgaaa acgttgccgg accatcatgc cagcgcacct
     2401 ccgtccgatt ccgacaggac agactagaga acgaggggcg agcgaatcat ccgctgacaa
     2461 gacagatgaa atttaagttg ccatctgctc ccgcaagaga ccggacgagc ttccacttaa
     2521 gattcaagac taacacaact cagtcgatca ggaatgtgaa taagctcaca tagcgacgtc
     2581 tggttctggc acttttcgga catggttcgg acatggtgct cgagcttgga gatgtccgtt
     2641 attggatgaa cagcggaaaa catctgctca cattgagctt ttccgatttt gacccatatc
     2701 ggaagtcgtc tcgacctcaa ttgacctaca ttagttcatg aaggcgtgtc atgctctcca
     2761 aagcgcttcc agcaaaacgg gatcgatcct atgatgaaac cgaccgatag gtttttagat
     2821 ataaatcgtc gttggatgct cgcagctttg acggcacttc cggccctgcc
//
LOCUS       DATMGX010000193         2253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_138241, whole genome shotgun sequence.
ACCESSION   DATMGX010000193 DATMGX010000000
VERSION     DATMGX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138241"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VNR65_03125"
     CDS             <1..1156
                     /locus_tag="VNR65_03125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWJ17700.1"
                     /translation="SGVLQEIKVTEGTTVGVNTVVGTISVDGESAAAPAKAAPAAEKP
                     AAKAEAKKIEERAPTPAPPPSAPAPVAHEEEEEARSSPLVRKIAREHGVSLSQISGTG
                     LGGRITKLDIMSFIESQGSSQPAPATPSAPAQPAAAAPAPRPAVPAAYPGDLVPVTNM
                     RKIIAQRMIESRRTSAHVHCMYEVDFTRIVNLRNKHKAGFEQRHGARLTFMPFFVRAA
                     VIALQQWPIINASLEGDNIRYHRQINMGIAVALDWGLIVPVLKNAGDLNFLGLQRGIT
                     DLGERARTKKLKPEDVEGSTFTVTNPGQFGAVFGLPIINQPNSAIMGVGGITKQPVVI
                     TDKDGADSIAIRSVVHLTLGYDHRLIDGAVADQFMDLVKKTLQTWSEEVG"
     gene            complement(1280..1564)
                     /locus_tag="VNR65_03130"
     CDS             complement(1280..1564)
                     /locus_tag="VNR65_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17701.1"
                     /translation="MNFLNQLLRGIAFIPALVNGIEGLFGGKSGAEKKDAAMSFLESA
                     LATVDAVAQREIVDPEQFKNGVSKIIDGTVDCLNASTWAKEKSPAPAVGK"
     gene            complement(1673..2143)
                     /locus_tag="VNR65_03135"
     CDS             complement(1673..2143)
                     /locus_tag="VNR65_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17702.1"
                     /translation="MTEECGVSKFRNRGGAEKTAPVTQYTAKDFQLEVLGKLAEVQNL
                     AESLDGSWNRKLEAVREQQLLKFDTRTLIAIGAIALSISGYVIQGARNSARQDTEIEA
                     TKARVVRLEQIAATNTEGRIRTEVQLVGLRDGQAEIKALIQAHDAANRKGALQT"
BASE COUNT          445 a          745 c          584 g          479 t
ORIGIN      
        1 ctccggagtt ctgcaggaaa tcaaagtcac cgaaggcacc acggtcggcg tgaacacggt
       61 cgtcggaacc atctcggtcg atggcgaatc tgccgctgct cccgccaaag cggctcctgc
      121 agccgagaag cccgccgcga aagctgaggc aaagaagatt gaagaaagag ctccaactcc
      181 tgctccgccg ccgtccgcac ccgcgcccgt cgctcacgaa gaggaagaag aagcccgctc
      241 ctccccgctg gtccgcaaga tcgctcgcga gcacggcgtc agtctctccc aaatttccgg
      301 caccggactt ggcggccgca tcaccaagct agacatcatg tcgttcatcg agagccaggg
      361 atcttcgcaa ccggctcccg ctactccatc tgcccctgct cagcctgcag ccgccgctcc
      421 ggcgccgcgc ccggcagttc ccgcagccta tccgggcgac ctcgtccccg tgacgaacat
      481 gcgcaagatc atcgcgcagc gcatgatcga gtcccgccgc accagcgctc acgtccactg
      541 catgtacgag gtggacttca cccgcatcgt caatctgcgc aataagcaca aagccggatt
      601 cgagcagcgc cacggcgctc gcctcacctt catgccattc ttcgtgcggg ccgccgttat
      661 cgccctgcaa cagtggccta tcatcaacgc cagcctcgaa ggcgacaaca tccgttatca
      721 ccgccagatc aacatgggca tcgctgtcgc cctcgactgg ggactcatcg tccccgtcct
      781 caagaatgcg ggcgacctga atttcctcgg cctgcagcgc ggcatcaccg atctcggcga
      841 gcgcgcccgt accaagaaac tcaaacccga ggacgtcgaa ggctccacct tcaccgtcac
      901 caaccccggc cagttcggcg cggtcttcgg actccccatc atcaaccagc cgaattcggc
      961 gatcatgggt gtcggcggaa tcaccaagca gcccgtggtg attaccgaca aggacggtgc
     1021 cgactcgatc gcgatccgct ccgtcgtcca cctgactctc ggctacgatc accgcctcat
     1081 cgacggcgcc gtagccgacc agttcatgga tctggtgaag aagacgctgc aaacctggag
     1141 cgaagaggtc ggctagggca gattccatca gggagagtgt tgttggactc tgtgtcctca
     1201 gtgtcctctg tggttcgcga ttttctcctg cggggtggcc catcctttcg cgttttttgc
     1261 gaaagggtgg ggattgcgat tacttaccta cggcgggagc cggagatttc tctttcgccc
     1321 aggtcgaagc gttgaggcaa tcgactgtgc catcgataat ttttgaaacg ccgttcttga
     1381 attgttccgg atcgacgatc tctcgctgcg ctaccgcatc cacggtggcc agtgcacttt
     1441 ccagaaacga catcgcagca tctttttttt ctgccccaga cttgccgccg aaaaggccct
     1501 cgatcccatt gaccagggcc ggaatgaaag cgattcctct caatagctga tttagaaagt
     1561 tcatagtgtt ctccttttca aagtgaatta cgcgcgaagc cgtgcctcct cgttcacaac
     1621 gaacaagagt tgcctttgtc tctaagtttt ccgattgtcc gcggtctgca atctatgttt
     1681 gcagagcgcc cttcctgttc gcggcgtcgt gcgcctgaat cagtgccttg atctcggcct
     1741 gaccatctcg cagtcctacg agttgaactt cagttcgtat ccggccttcc gtattcgttg
     1801 cggcaatctg ctccagacgc accacccgcg ccttcgtcgc ttcgatctca gtgtcttgcc
     1861 gagcggagtt gcgcgcgcct tggatcacat aaccgctgat agaaagcgca atcgctccta
     1921 ttgcgatcag agtccgagtg tcgaacttca gaagttgttg ttcccggact gcctcgagct
     1981 tccgattcca ggacccgtcc aaagactcag cgaggttttg cacctccgcc agcttgccca
     2041 gaacttcaag ctggaaatcc ttagccgtgt actgcgtcac cggcgctgtt ttctccgcgc
     2101 cgccccgatt ccggaacttg cttaccccac actcttcagt cataacggat aatccacatg
     2161 caacgcggct gcgtatcgcg agtatcgcgg cggcgaagaa ctgtcataca gtctcagaaa
     2221 gtagctttgt gtccgcgcca gtcgcggcag gct
//
LOCUS       DATMGX010000194         2662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_79553, whole genome shotgun sequence.
ACCESSION   DATMGX010000194 DATMGX010000000
VERSION     DATMGX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79553"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            595..753
                     /locus_tag="VNR65_03140"
     CDS             595..753
                     /locus_tag="VNR65_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HWJ17703.1"
                     /translation="MGGLLWSIITVLFVLWLIGFVMHFGGGLIHLLLVIAVVLFVVNL
                     LTGRGARV"
     gene            868..1110
                     /locus_tag="VNR65_03145"
     CDS             868..1110
                     /locus_tag="VNR65_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17704.1"
                     /translation="MGIKDALEKAVGNTKNTIDNVKDAVSESGHRAAAKAEETKRSVE
                     GDQMSVTEKAGSMFNQAKETVKAKVDETKQDIRKNT"
     gene            1439..1597
                     /locus_tag="VNR65_03150"
     CDS             1439..1597
                     /locus_tag="VNR65_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17705.1"
                     /translation="MSILVLLIVLILVFGVGGGVYNKGAYRNYGYGGGGLLLIILIIL
                     LVSGYVHV"
     gene            complement(1627..1977)
                     /locus_tag="VNR65_03155"
     CDS             complement(1627..1977)
                     /locus_tag="VNR65_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17706.1"
                     /translation="MAAATSRPIDLARLAIVDLNPVLAVLVRKYPLPVSAGAIGHVQS
                     EAAGERLMASLERLLTKRLRLKINREKSGCVAVESRAPIVLLQIAVTRGVFKAHRVPR
                     TPTNASSALAKAQS"
     gene            complement(2104..2448)
                     /locus_tag="VNR65_03160"
     CDS             complement(2104..2448)
                     /locus_tag="VNR65_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17707.1"
                     /translation="MRNLARRADRLKRTVEKVRDRVADAGVRASEMLPSRAGRGGQLR
                     DLLSKYPLPIGVGAIVLGFVAGFALPSTQFENRRLGKLSDRTKARLQQRVQERLENVT
                     DVVRHAAKTAGR"
BASE COUNT          554 a          799 c          804 g          505 t
ORIGIN      
        1 aagtcgatgc tcttaggctt tggcaaagaa ctcaatccag gtctgccata tagagacttc
       61 ccccggtatc gactccctgc ggcgcgcccg aacggcgcgc gaatcgaggg cagataaccc
      121 aaatagaagc atgctagcag agcgcgcccg agcagctccg gcgatcccca cgcactactt
      181 ggcgcgaatc gcgctgtgcc gccgaagacg cagtacgcgc gcccggatcg ctacgctcca
      241 tttgtcgttc gttgcctggg taaaaatcgc gagaccgtcg actgtcacgc caagacgacg
      301 aagatgacta ccgtgcaacg tcgggcccca cactcgacgc atgcggcatc gcagctgcga
      361 cactgtgccg gcggacagcc tatctccctc cgcactcgct cgtcgggctc gcccactgag
      421 gttctggcgg cccgaagact ccattgaggg tggggtgaga aaggtagttg cgggtcgagg
      481 agcggcgtga cacaatattt atacgggagt ctgcgcgcaa gtcactgtgc gcccgggaag
      541 atgcgaagtc gccggagtca ttcgggctgt gacccgagca aaaaggagga cttcatgggc
      601 ggtttgctct ggtccatcat tacggtattg ttcgtgctct ggctgattgg gttcgtgatg
      661 catttcggag gcggactgat ccacctgttg ctcgtgattg cggtggtctt gttcgtcgtc
      721 aatctgctta ccgggcgcgg tgctcgagtc taaggcgatc cgcagcgttc gcgtttgccc
      781 agaggtctct tactcgacag cagtcgcccc gcggatggtc taatccagtt tctgcgtcct
      841 cagaggcgca gcaagaaagc gatgcaaatg ggaattaaag acgcgttaga aaaagccgtc
      901 ggcaacacca agaataccat agacaacgtc aaggatgcgg tgagtgagag cggtcatcgc
      961 gcagcagcga aggcggagga aacgaagcgt tccgtcgagg gcgatcagat gagcgttacc
     1021 gaaaaggccg gttcgatgtt taatcaagcg aaggagacgg tgaaagctaa agtcgacgaa
     1081 acgaaacaag acatccgcaa gaacacgtaa gcaaacgccg cccgaaccgg cgaactcgtc
     1141 atcgccgaac acggcgtagc gcgcggagcc gaaaccgcct cactcgcaat cgcgactcat
     1201 cgtcgacggc gttgtgaggc ggcccggtcc cggtctttgg ctatagtttc atcggaagtc
     1261 atcgtccaag agcggttatg ggcctcgaac aagcacgatg ggcagcagag cgacagcgag
     1321 gacgatcgca tcagcacgcg gttcggcaat gcggtcggct tcgggcgcga tagcggggtt
     1381 gcatcaaaac ggattacccg gattgccctc ttgggcgata caatcgaaag aggctcctat
     1441 gtcgatccta gttctgctaa tcgtgctcat tctggtgttc ggcgtcggcg gcggcgtcta
     1501 taacaagggt gcctatcgca attacggtta cggagggggc gggttgctgc tcatcattct
     1561 catcattctg ctcgtgagcg ggtacgttca cgtctaaata aaagcgcatg gcagacaacc
     1621 ccgacgctac gactgcgctt tcgccagcgc cgacgaagca ttcgtcggcg tacgcggcac
     1681 ccgatgcgct ttaaaaacgc cgcgcgtgac cgctatctgc agcagcacta tcggagcgcg
     1741 cgattctacg gccacgcaac cgctcttttc gcggttaatc ttcagccgaa ggcgcttggt
     1801 caaaaggcgc tccagcgatg ccatcagacg ctctcccgcg gcttctgact gcacgtgacc
     1861 gatcgcgccg gcgctgaccg gcagcggata tttcctcacg agcaccgcca aaacgggatt
     1921 gagatcgacg atggccagcc gcgcgagatc aatcggtcgc gatgtcgcgg ctgccacacc
     1981 ttgagcgtct cgtcagcgga ggccgagaca acgcgtcggc tgcgccttgt aggactcgga
     2041 aatcgttacc ggcccaggca ccacgctacg gcggacgcgg ggcttgggtt cgtcaaagcg
     2101 acgttagcgc cccgcggtct tcgcagcgtg ccgcaccaca tccgttacat tctccagccg
     2161 ctcctggacg cgttgctgca gacgcgcctt ggtgcggtcg gagagtttgc ccaaacgccg
     2221 gttctcgaac tgcgtcgatg gaagagcgaa acccgcgacg aacccaagga cgatcgcgcc
     2281 gacgccgatc ggcagcggat atttgctcag gagatcgcga agctgcccgc cccggccggc
     2341 tctcgacggc agcatctcgc tcgctcgcac gccggcgtcc gccactcgat cccgcacctt
     2401 ttcgactgtt cgtttgaggc ggtctgccct ccgagccagg tttctcatga atctcccttt
     2461 tcgattctga agccttcgcc tttttctgat atcctatgaa tctcgcgaag ctcgcagaaa
     2521 gcgtccggtc gaaaatccga gcaagaaccc cgtcaccgtg aaggtcagcg gaacgcgcct
     2581 cgccagcgac tcgacatcgg cgagcaaacg atcggtgtcg gatgcgctga gataacggcc
     2641 ggcggcgtcc agacgttggg cg
//
LOCUS       DATMGX010000195         4946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_54506, whole genome shotgun sequence.
ACCESSION   DATMGX010000195 DATMGX010000000
VERSION     DATMGX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54506"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2271
                     /locus_tag="VNR65_03165"
     CDS             <1..2271
                     /locus_tag="VNR65_03165"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HWJ17708.1"
                     /translation="MLIPISQQIWDAKYRFSPPGGRAEQSIEETWTRVARAAASFEPE
                     QSRKGWEHRFLEALADFAFIPAGRILSGAGTGRDVTLFNCFVMGEIGDDMGSIFANVR
                     EAALTLQQGGGIGHDFSTLRPKGAHVRGVGADASGPVSFMDVWDSMCKTIMSAGARRG
                     AMMGTMRCDHPDIEAFIEAKADPARLRNFNLSVLVTDAFMEAVKEDRSWPLVFGGEVY
                     RTVNAHGLWRKIMRSAYDYAEPGVIFIDRVNALNNLGYCEPISATNPCGEQPLPPYGA
                     CLLGSINLAALIAEPFSARAALDQKRLEDLVPVAVRLLDNIIDVSRYPLPQQEREAKA
                     KRRIGLGVTGLADALILCGAHYGSEKGRDLAASWMAQISHAAYAASAELAKEKGAFPL
                     FDAMQFLRRPFPGSLPPSLQKTIKDKGLRNGVLTSIAPTGTISLLAGNVSSGIEPVFD
                     FEYRRRLLSPDGSERHEIIRDYAVRLFRDTHGDTPLPDAFVTAHDLTPQQHLLMQAAL
                     QSHVDASISKTVNCPEAIAFEDFEMLYLEAYRLGLKGCTAYRPNEVTGAVLSPAPPAA
                     QAGQALQPSLPLASANILPKARDTVLAGFTYKLKWPGSDHAIYVTINDTFDHGQRRPF
                     EIFINSKNLEHYAWTVALTRMISAIFRRGGDVSFVVEELKAVFDPRGGAWTSGKYVPS
                     LLAAIGEIIEQHFIHIGFQAESTRESGTHDEKIKDQEATAFETFSPSSFCPRCGSPSM
                     IYQGRVCRNCGHSTCS"
     gene            2378..4333
                     /gene="acs"
                     /locus_tag="VNR65_03170"
     CDS             2378..4333
                     /gene="acs"
                     /locus_tag="VNR65_03170"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWJ17709.1"
                     /translation="MTVTVKTYPVPESWSSSAWIDNEKYRAMYDQSVGDPEGFWGEQA
                     SRVDWIKPFTKVKNTSYDPHNLSIKWYEDGTLNVCANCVDRHLEKRGDQVAIVWEGDE
                     PNMDLKITYRQLYERVSRFANVLKKHGVKKGDRVTIYLPMIPEATYAMLACARIGAVH
                     SVVFGGFSPDSLASRIVDCDSTVLITADEGVRGGRKVPLKANADKALEKCPTIERVLV
                     VKRTNSPVAWTPDRDFWLGEEMQSVSADCPPEEMNAEDPLFILYTSGSTGQPKGVLHT
                     TGGYLVYAAITHQYVFDYKDGDIYWCTADVGWVTGHSYIVYGPLANGATTLMFEGVPN
                     YPSPSRFWDVVDKHQVNTFYTAPTAIRALMGAGEQHVKKTSRKSLKLLGSVGEPINPE
                     AWEWYYHIVGDERCPIVDTWWQTETGGILITPLPGAIATKPGSATLPFFGVKPALMAP
                     DGQVIEGAGEGNLVILDSWPGQMRTVFRDHNRFVQTYFAAYPGTYFTGDGCKRDADGY
                     YWITGRVDDVINVAGHRMGTAEVESALVAHPKVAEAACVGYPHSLKGQGIYVYVTLMS
                     GIEPTEALRTELRNWVRQEIGPIATPDLIQFAPGLPKTRSGKIMRRILRKIAEDDFSN
                     LGDTSTLAEPAVVSDLIENRMNKPAVA"
BASE COUNT         1035 a         1460 c         1443 g         1008 t
ORIGIN      
        1 atgctgatcc cgatttccca gcagatttgg gacgccaagt accgcttttc gccgccgggc
       61 ggacgtgccg agcagtcgat cgaggagaca tggacaagag ttgcgcgtgc cgctgcctcc
      121 tttgaaccgg agcagtctcg caaaggctgg gaacaccgct ttctggaggc cttggcagac
      181 ttcgcattca tccccgctgg ccgaatactg tcgggagccg gaaccggccg cgatgtgaca
      241 ctcttcaact gtttcgtcat gggcgagatc ggagacgaca tgggctcgat cttcgccaat
      301 gtcagggaag ccgctctcac attgcagcag ggcggcggca ttggtcacga tttctcgacg
      361 ctcaggccga aaggcgcgca tgtgcgcggc gtgggcgcgg atgcttctgg tcccgtcagc
      421 ttcatggatg tctgggactc catgtgcaag acaatcatgt cggcgggtgc gcggcgcgga
      481 gcgatgatgg gcaccatgcg ctgcgaccac cccgatatcg aggctttcat cgaggcgaaa
      541 gccgacccgg cgaggctccg caatttcaat ctgtctgttc ttgtcacgga cgccttcatg
      601 gaagcggtaa aggaggacag gtcctggccg cttgtctttg gcggcgaggt ttatcgcacg
      661 gtgaacgcgc acggcctttg gcgaaagatc atgcgcagcg cctatgatta cgccgagccc
      721 ggcgtgatct ttatcgaccg cgtgaatgcg ttgaataatc tcggctattg cgaaccgatc
      781 agcgctacga acccctgcgg agaacagcca cttccgcctt atggcgcctg cctcctcggc
      841 tccatcaatc tggcggcgct catcgcagag ccgttcagcg ccagagcggc cctcgatcag
      901 aagaggttgg aagacctcgt gccggtggcg gtccgattgc tcgacaatat catcgatgta
      961 tctcgctatc ctctgcctca acaggagaga gaggctaagg ccaaacggcg catcgggtta
     1021 ggcgtaaccg gactcgccga tgcactgatc ctgtgcgggg ctcattacgg ctcggagaaa
     1081 gggcgcgatc ttgcagcatc atggatggcg cagatcagtc acgctgctta tgcggcaagc
     1141 gccgaactgg cgaaggaaaa gggagcattc cctctctttg acgcaatgca attcttgcgg
     1201 cgcccctttc cgggttcgct cccgccttcg cttcaaaaga cgatcaaaga caaaggcctg
     1261 cgcaacggcg ttttgacctc tatcgctccg accggcacca tctcgctgct cgctgggaac
     1321 gtgtcgagcg gaattgagcc cgttttcgat ttcgagtacc gccgccgcct tctctcgccg
     1381 gacggttccg aacgccacga aatcatccgc gactacgcgg tgcggctgtt ccgcgacacg
     1441 catggcgaca ctcctcttcc ggatgccttc gtaacggctc atgatctcac accgcaacag
     1501 cacctcttga tgcaggccgc gctccagagc catgtcgatg cgtccatttc gaaaacggtt
     1561 aactgcccgg aggcgatcgc ctttgaagac ttcgagatgc tttatctcga agcctaccgc
     1621 ctcgggctca aaggatgcac cgcctaccgt ccgaacgagg tgacgggtgc agtgttgtca
     1681 ccggcgcctc cagcagccca ggcaggacag gccctgcaac cctctctccc gctcgcgtca
     1741 gctaatattc tgccaaaggc acgcgacacg gtgctggcag gcttcaccta caagctcaaa
     1801 tggccgggct cggaccacgc gatctatgtc acgatcaatg acactttcga tcacggccaa
     1861 cgccgtccgt ttgaaatctt catcaattcc aagaacctgg agcactacgc ctggacagtc
     1921 gcgctcacac gcatgatcag cgcaatcttc cgccgcggcg gagatgtttc cttcgttgtg
     1981 gaggaactga aggccgtttt cgatccgcgt gggggcgctt ggacgagcgg caagtatgtg
     2041 cccagtctgc tcgccgcgat cggggaaatt attgagcagc acttcattca catcgggttt
     2101 caggccgaga gcactcggga aagcggaact catgatgaaa agatcaagga tcaggaagcg
     2161 accgccttcg agactttttc gccttcaagt ttctgtccac gctgcggatc tccgagtatg
     2221 atctatcagg gccgcgtgtg ccgcaattgc ggacactcca cctgcagctg agtttgcaga
     2281 aacgaagaag ggcaacctct tcgaccattg cactgttgcc cttaagccag gcaatcaaca
     2341 taatgtcggc gaagccgcat atttagggag gatgtcgatg acggtgacgg tgaagaccta
     2401 tccggttcca gagagctgga gctcatcagc ttggatcgat aatgaaaaat accgggcaat
     2461 gtacgatcag agcgttggtg atccggaggg gttttggggc gagcaggctt cccgcgtcga
     2521 ctggatcaag ccattcacaa aggtgaagaa cacctcctac gatccgcaca acctatccat
     2581 caaatggtac gaggacggga cgctgaacgt ctgcgcgaac tgcgtagatc gtcacctgga
     2641 aaaacggggc gaccaggttg ccatcgtttg ggaaggcgac gagccgaata tggatctcaa
     2701 gatcacatac cggcagctct acgagcgcgt tagccgtttt gcgaacgtgc tcaagaagca
     2761 cggcgtcaag aagggcgacc gcgtcacgat ctacctgccg atgatccccg aagccactta
     2821 tgcgatgctg gcctgcgccc gcattggagc ggtccactcg gtcgtgttcg gcgggttctc
     2881 accggacagt ctcgcgagcc gcatcgtcga ctgcgattcg accgtgctga ttaccgccga
     2941 cgaaggcgtc cgaggcggcc gcaaggttcc gctcaaggcg aacgccgaca aggcccttga
     3001 gaagtgcccg accattgagc gcgttctcgt ggtaaagcgc acgaattccc ccgttgcctg
     3061 gacgccggac cgcgattttt ggctcggcga ggagatgcag agcgtttcag ccgattgccc
     3121 acctgaagag atgaatgcgg aggatccgct tttcatcctc tacacctccg gctccaccgg
     3181 ccagcccaag ggcgtgcttc acacaaccgg cggctatctg gtctacgcgg cgatcacgca
     3241 ccaatacgtc ttcgattaca aggacggcga tatctactgg tgcacggcgg acgtcggctg
     3301 ggtcaccggc cacagctaca tcgtctacgg cccgctggcg aatggagcga ccacgctcat
     3361 gttcgagggc gttccgaatt atccgagccc ctcccggttt tgggacgtcg tcgacaagca
     3421 tcaggtcaac accttctata ccgcgccgac cgctatccgg gctctcatgg gagcaggcga
     3481 gcagcatgtc aaaaagacaa gccgcaagtc gctgaagctg cttggaagcg tgggcgagcc
     3541 gatcaatcct gaagcctggg agtggtatta tcacatcgtg ggcgatgagc gctgcccaat
     3601 cgtcgatacc tggtggcaga cggaaacggg tggaattctg atcacgccgt tgcctggcgc
     3661 cattgccacg aagcccggct cggcgacgct gccgttcttc ggcgtcaagc ctgcgcttat
     3721 ggctcccgat ggccaggtga ttgagggggc gggtgagggc aatctcgtca tcctggactc
     3781 ctggccgggc cagatgcgca cggtcttccg cgaccacaat cgcttcgtgc agacctattt
     3841 cgccgcttat ccgggaacct acttcacagg tgacggttgc aagcgggatg ccgacggcta
     3901 ttactggatc acgggccgcg tcgatgacgt gattaatgtg gccggccatc gcatgggtac
     3961 ggcggaagtg gaatcggctc tcgttgccca tccgaaggtc gccgaagcag cctgcgtggg
     4021 ctacccgcac agcctgaaag gccaaggcat ctacgtctac gtcactttga tgagtggaat
     4081 cgagcctacc gaagcgctca gaaccgagct tcgcaactgg gttcgccagg agatcggacc
     4141 gatcgccact cctgacctga ttcagttcgc gcctggtctt cccaagaccc gctcgggtaa
     4201 aatcatgcgc cgcatcttgc ggaagattgc cgaggacgat ttctcgaacc tcggcgacac
     4261 gtccacgctg gccgaaccgg ccgtcgtgtc cgacttgatc gagaaccgca tgaacaagcc
     4321 cgcggttgct taagaacacg tgcccggcgc ctccgccggg cacgtttcct gccccgcgcc
     4381 tcaagatcgc gctcggggaa cgtgcttatc cacaggttgc tcagagtgcc acaaccttag
     4441 gtcggcataa acgaatgtca gcgtaagatt ttgtggcgat tcagtcacgc tcacgatgca
     4501 gaaatgcctc tcgcttcagc gaggcatgac tcccgcaagc aagtttgcgg taatgagaat
     4561 ggatcgggtc atagtgcagc ttaggatttg gcgatggttg agcggaaagt tgtagagagc
     4621 attcgtgata aactggcttc tggcgttcgc tcgccactgt tgatgccaat catggaagga
     4681 aacactctcg aaagtgacga cgtagccgag ttcctcaagg gccgtttgtc tcatttgcgt
     4741 gagaagtcct gtccccagcc gcgggtaaga ctaagctaag aggcgagatt tttcgccagc
     4801 gccatcgtct gctctcttca tgcggcaact aggtcatgca acacgccgcg ggatagagat
     4861 taaagacgtt gatcgaggtt gccgcctagc ctagtgttcg cgacacttaa ggcttattac
     4921 gaacaccact caatcaaata taggtt
//
LOCUS       DATMGX010000196         3581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_7261,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000196 DATMGX010000000
VERSION     DATMGX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7261"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VNR65_03175"
     CDS             complement(<1..372)
                     /locus_tag="VNR65_03175"
                     /inference="COORDINATES: protein motif:HMM:NF018867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWJ17710.1"
                     /translation="MQFSTRLLHLCAALLVLLSLTLPHPTLAQDGSTSLQGVVEDISG
                     ARIAAAVITVVDPAKGFRLEAVANAQGQFIFGMLAPGRYDVSAAAPGMTTKTTHGVEL
                     YVGGVSVVQVRLALAGLTQTVR"
     gene            499..777
                     /locus_tag="VNR65_03180"
     CDS             499..777
                     /locus_tag="VNR65_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17711.1"
                     /translation="MYFRFRKGFFPEPEWGLLLGGGGAEQDPILSGRTHIKVPEGYPL
                     DLYWKGASPKEEYNYVLVDVQPGKPTKFALNRFRPWSAVPFATVELFK"
     gene            924..2573
                     /locus_tag="VNR65_03185"
     CDS             924..2573
                     /locus_tag="VNR65_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWJ17712.1"
                     /translation="MDFGAGVRKLTLGALAISSFLLASGGAPSAAQTAKQPNILVIMG
                     DDIGMWNIGAYHRGLMAGKTPNLDKLAKEGMLFTDYYAEASCTAGRANFITGELPIRT
                     GMTTVGQAGAKIGLPAEAVTTATILKSMGYATGQFGKNHLGDLNEYLPTVHGFDEFFG
                     YLYHLDAMEDPAHPNYPQNLLNVVGPRNMVHSWATATDDPTVMPRWGKVGKQKIEDAG
                     PLYPKRMETVDDEIRDLALKFIDKAKADNKPFFVWLNPTRMHIVTHLSPKYEALRNAE
                     NGWSEEEAGMAQLDDDVGIVMNKLKDLGEDDNTIVVFTTDNGTEVFTWPDGGQTPFAQ
                     SKGTVLEGGFRVPCLLRWPGHVPADSVQNGIFSGLDWLPTFVAAAGNPNVTEELLKGK
                     TIGDRTYKNHLDGYNQMAAITGKGPSVRHEIFYLGESTVGAVRIDDYKFRFIDQPAGW
                     LGEKTHPDVPYITNLRLDPFERTGWPSNGTKDGAQQYFDWFKYQFWRFVFVQQAMGKE
                     IQTFLDYPPMQRGASFNLDAIKAEMGKRMAEAEAAAKGPSN"
     gene            2655..3359
                     /locus_tag="VNR65_03190"
     CDS             2655..3359
                     /locus_tag="VNR65_03190"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HWJ17713.1"
                     /translation="MAGRAKKSLVLASLAILWCLRSSAQTAQVLPEVDTYFKLSSELR
                     VSFQAKETREAGDPIHAEIGPSLDFYLKPLIRLKKATEFDLDDSKSRPLVFSVGYRYL
                     PQANNAPATNRMEPVVTLNFPLKGRFLLSDRNRADLDWRNGNFTWRYRNRIQIEKTLT
                     LRSYHPSPYASAEFFYQSQFGKWSDTAIYAGCIFPIGKRLEVNPYYEHQNNTGNSPNQ
                     QLNQLGLMLNLYFSVR"
BASE COUNT          812 a         1050 c         1037 g          682 t
ORIGIN      
        1 cctgaccgtt tgcgtgagtc cggctagcgc caaccgtacc tgcaccaccg acacgccgcc
       61 gacgtaaagc tccacgccat gggtcgtctt ggtggtcatg ccgggcgctg ccgcgctcac
      121 gtcatagcgg ccgggcgcta acatgccgaa gatgaattgt ccttgcgcat tggccaccgc
      181 ttccaagcgg aagccttttg cgggatccac gacggtaatt acggcggcgg cgatccgggc
      241 gccgctgatg tcttcgacca cgccttgcag cgaggtggat ccatcttggg cgagtgtggg
      301 atgcggaagc gtcaggctca atagaaccaa aagcgcggcg cagaggtgga ggaggcgagt
      361 cgaaaattgc atggcgcgaa ttccccaatc aggcgacgtt cccatagcag tacaacccag
      421 ctacacatat aggagggttg aagcgaggca gtctgtgatt gggacttcac gaaaggccaa
      481 gaccaaaaag ggaaagcatt gtactttcgt tttcgaaagg gatttttccc agaaccagaa
      541 tggggcctgc tgctgggcgg cggcggtgct gaacaggatc cgatcctttc cggtagaacc
      601 catatcaagg tacccgaggg ctacccgctt gatctgtatt ggaagggcgc gagcccgaaa
      661 gaggaataca attacgtcct cgtggacgtg cagcccggaa agcccacaaa gttcgctctc
      721 aatcgcttcc gcccatggtc ggctgtgccg ttcgcgacgg tggagctttt caagtgaagg
      781 tccgcagcgc tgtgctttta tggatttggt cgacttttga tcgttgaggg tgaacctcct
      841 tccgagggtt ccccattcgg atgatctcat tcgcaaccct gcccctagcg gggctaacca
      901 caggtacttg aaaggataaa agtatggatt ttggtgcagg agttcgaaaa ctgacgctgg
      961 gagcgctggc catctccagc ttcttgctgg cgagcggcgg cgccccaagc gcagcgcaaa
     1021 ccgcgaagca gcctaatatt ctcgtcatca tgggtgacga catcggcatg tggaacatcg
     1081 gcgcttacca ccgcggcttg atggccggca aaacgccgaa cctcgacaag ctcgccaagg
     1141 agggcatgtt attcaccgac tattacgccg aggcgagttg cacggcgggt cgcgcgaact
     1201 tcatcaccgg cgaacttccg attcgtacgg gcatgaccac cgtaggccag gcaggcgcta
     1261 aaatcgggct acccgcggag gctgtgacta ccgcaacgat actcaagtcc atgggctatg
     1321 ccaccgggca gttcggtaag aatcaccttg gcgatctcaa tgagtatctg cccaccgttc
     1381 acggtttcga cgagttcttt ggctacctct accacctcga cgcgatggaa gacccggcgc
     1441 atcctaacta tccgcagaat ctcttgaatg tcgttggccc gcgcaacatg gtgcattcgt
     1501 gggcgactgc tacggatgac ccgaccgtga tgcctcgctg gggcaaggtc ggcaaacaga
     1561 agattgagga cgccggcccg ctctatccca agcgcatgga gaccgtggat gacgagattc
     1621 gcgacctcgc cctcaaattc atcgacaaag ccaaggccga caacaagccg tttttcgtct
     1681 ggctcaaccc gacccgcatg catatcgtca cgcacctctc gcctaaatac gaggcgctgc
     1741 gcaatgccga aaatggctgg tcggaagaag aagccggcat ggcgcagttg gatgacgacg
     1801 tcggcatcgt gatgaataag ctgaaggacc tgggcgaaga tgacaacacc attgtcgtgt
     1861 tcaccacgga caacggcacc gaagtcttca cctggcccga tggcggccag acaccgttcg
     1921 cgcagtccaa gggaacagtc ctcgagggcg gcttccgcgt gccctgcctc ctgcgctggc
     1981 ctggtcatgt gccggcggac tcggtgcaga acggcatctt ctccggcctg gactggttgc
     2041 ccacattcgt tgccgcggcc ggtaatccca acgtcaccga agagttgctg aaaggcaaaa
     2101 cgatcggtga ccggacctac aagaaccatc tggacggcta caaccagatg gccgccatca
     2161 ccggcaaagg cccgtccgtg cgccatgaaa tcttctacct cggcgaaagc acggtcggcg
     2221 cggtgcgcat agacgattac aagttccgct tcatcgacca accggccggc tggctgggtg
     2281 aaaagaccca tcctgacgtg ccttacatca ccaacctgcg tctcgatccg ttcgagcgca
     2341 cgggctggcc cagcaacggg acgaaagacg gggcgcagca atacttcgat tggttcaagt
     2401 accaattctg gcgcttcgtg ttcgtccagc aggcaatggg caaggaaatc caaaccttcc
     2461 tcgattaccc gccgatgcag cggggcgcga gcttcaacct tgacgccatc aaagcggaga
     2521 tggggaaaag gatggccgag gcggaggcgg cggccaaagg ccccagcaac taacaggaac
     2581 gacctttacg gcgggcggct cgcaagggcc gcccgttcgt ttgtcagttt ctgtacaccc
     2641 atcaaaacca aaccatggct ggccgtgcaa aaaaatcgct ggtgctggcg tcgctcgcga
     2701 ttctctggtg cctgcgcagc agcgcgcaaa cggcgcaagt gctgccggaa gttgacacct
     2761 acttcaagct gagttccgag ctgcgggtca gcttccaggc aaaggagacg agagaggccg
     2821 gcgaccccat tcacgccgag atagggccca gccttgactt ctatctgaag ccgctgattc
     2881 gtctcaagaa agccactgag ttcgatctgg acgactcgaa gtcccggcct ctggtgtttt
     2941 ccgttggata ccgctatctg ccgcaggcca acaatgcgcc ggcgaccaac cgcatggagc
     3001 cagttgtaac ccttaacttt cccctgaaag gaagatttct actctccgat agaaatcggg
     3061 cggacctgga ctggcgaaac ggaaacttca cctggcgcta ccgcaaccga attcagatcg
     3121 aaaagacgct tacgttgcgc tcgtaccacc cttcaccgta cgccagtgcc gagttcttct
     3181 atcagagcca gtttggaaaa tggagcgaca ctgcgatcta cgccggctgc atctttccca
     3241 tcggcaagcg cctggaggtt aacccgtact atgagcacca gaacaacacc ggcaacagcc
     3301 ccaatcagca actgaatcag ttggggctta tgctcaatct ctacttttca gtcaggtaac
     3361 ggtgatttag cgatcggcgt ggcgcggact ggagggcaag gggtcactgg acgtgtcagc
     3421 ggccatccgg aagtgatatt gcgatctccc gagcagcgaa gcgcccactg ggccggaaga
     3481 gagcgacggc tcagtagaac caatagcgca gcgcagcgat agcgatgttc gcagattata
     3541 ggcctaatgc cctgaatgca ttggacgaat gtcaagaagg t
//
LOCUS       DATMGX010000197         4550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_224299, whole genome shotgun sequence.
ACCESSION   DATMGX010000197 DATMGX010000000
VERSION     DATMGX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224299"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VNR65_03195"
     CDS             <1..324
                     /locus_tag="VNR65_03195"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HWJ17714.1"
                     /translation="VAQIRGQQVEFMTDTGATYVALSYETAQKLGLTPQNLRFNGRST
                     TANGVARVASVVFETVRIGDITVKDVQAVIAEPGRMTQNLLGMSFIRQLSGFELNGAR
                     LTMIE"
     gene            complement(374..1846)
                     /locus_tag="VNR65_03200"
     CDS             complement(374..1846)
                     /locus_tag="VNR65_03200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWJ17715.1"
                     /translation="MTFQNLKLSEKVLAAIEAAGYTAPTPIQAQAIPFALEGRDVVGI
                     AQTGTGKTASFVLPLLTMLEKGRARARMPRSLILEPTRELAAQVAEAIEALGSHHKLT
                     VALLIGGVSFDDQEKKLERGADILIATPGRLLDHFGRGKLLMNAVDYLVIDEADRMLD
                     MGFIPDIERICKLLPPSRQTMFFSATMPPEIQKLTEQFLRNPEQIRVSAPATNAKLIT
                     QKLKFSPADSKTKRHVLRELMRNEAGAVKNAIIFCNRKRDVAVLHKSLIKHGFNAAAL
                     HGDLDQRQRMATLEAFRKGEITYLAASDVAARGLDIPDVSHIFNFDVPISPEDYVHRI
                     GRTGRAGREGFTAMIVTPKEMKGLRAIESLCKAKIEWLDGEPSTENLTSADFSERRER
                     GTRRGDRSKGARKDQAAPEQRQSAEVRPIRSGPEPRQPAEVRPVPSGAGAGQRRERNN
                     DRSSVSRYHRELLPPATSAPVVGLGDHVPEFLKRPIRAAG"
     gene            complement(2149..2757)
                     /locus_tag="VNR65_03205"
     CDS             complement(2149..2757)
                     /locus_tag="VNR65_03205"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWJ17716.1"
                     /translation="MSIPPQPLDREALSMDTAALARFLIGKLVVRAFPDTLATARIVE
                     TEAYLTGDEASHGFRGPTPRNKVMFGPPGFAYVYLAYGVSFMLNVSSAAAGAGEAVLI
                     RAAEPVTGLEAMRERRGDVPTRDLLRGPGRLAKALDIDRSLDGTDLCAAGSLWLASDG
                     EAFGAIGVSVRIGITKDAVRPLRFYLKGNKFVSGPASLNAAL"
     gene            3331..>4550
                     /locus_tag="VNR65_03210"
     CDS             3331..>4550
                     /locus_tag="VNR65_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17717.1"
                     /translation="MLKEDQIALDDPRCDKILATGQTIKRRIFLFSAFIIVFICIVAV
                     ALVAEQRQSALDRASAHSANLSAAFEEQVRRVLDSVSGAMDLIVSRIEKEGSDFDLSQ
                     WAPLIPGVAISTIQVAIIGPDGMLSASSLSKNPKPVDLSDREHFRVHRDNPNLGLFIG
                     KPVLGRVSNQITIQVTKRLQARDGSFGGVLVFALNPDYLTTLHRQVDLGQAGNVTVFG
                     LDSVIRARFTSSNSSEAPGVGSSMAAGRSFKDAAFADAGTHTSASVIDGVVRIFNWRK
                     VAGYPLIVTVGLGKAEALSAADHQALLILGIAASAVLLVGLMAVRLSREISNRVTHEI
                     ALYYEGERLRAAHDCLTDQHERLIAKSALLAEERIKLQETNAQLSLAQQRSEAASKAK
                     SAFLANMSHELRTPL"
BASE COUNT          975 a         1277 c         1301 g          997 t
ORIGIN      
        1 gtcgcccaga tcagaggtca gcaggttgaa ttcatgaccg atacgggcgc tacctacgtc
       61 gcgctcagct atgagacagc tcagaagctg ggactaacac cgcagaatct ccgctttaat
      121 ggtcggtcga cgacggccaa cggtgttgcc cgcgtggcgt ccgtcgtatt cgagacagtc
      181 cgcattgggg acattacggt caaggatgtt caggccgtca tcgccgagcc gggccggatg
      241 acacagaacc ttctcggcat gagcttcatc cggcagctgt cgggatttga gctaaacggc
      301 gcaagactca caatgattga gtagcgccga gttttcggcg gcggacatgc aggaaagcgg
      361 cggaccgaac gcgttaaccc gccgcgcgga tcggccgctt cagaaactcc ggtacgtggt
      421 cgccaagccc gacaacgggt gctgatgtgg ccgggggcag caattcgcga tgataccgcg
      481 acacgctgct ccgatcattg ttcctctcgc gccgctgccc tgcgccggcg ccgctgggga
      541 caggccgaac ctcagcgggt tgccgcggct ctggcccgct ccgaatgggg cgaacttcag
      601 cggattgacg ttgctctgga gcggcctgat ccttgcgtgc gcctttggag cgatctcccc
      661 ggcgcgtacc acgctcgcgg cgctcgctaa agtccgctga cgttagattc tcagtggatg
      721 gctccccgtc gagccactct atcttggcct tgcaaagcga ttcgatagcg cggagccctt
      781 tcatttcctt tggcgtcacg atcatcgccg taaatccctc acggcctgcg cggcctgtgc
      841 ggcctatgcg gtgcacgtaa tcctcggggc tgatcggaac atcgaaattg aagatgtggc
      901 tcacatcggg aatatcgagc ccgcgcgctg cgacgtcgga agcggcaagg taggttattt
      961 cccctttgcg gaaggcttca agtgtcgcca tgcgctgccg ctgatcgaga tcgccatgca
     1021 aagcagctgc attgaaccca tgcttaatca gcgacttatg cagaacagcg acgtcccttt
     1081 tccgattaca gaagatgatc gcgtttttga ccgcacctgc ttcgttgcgc atcagttcgc
     1141 gcagaacatg gcgctttgtc ttcgaatcgg caggggaaaa ctttagtttc tgggtgatga
     1201 gcttagcgtt cgtcgcggga gcgcttacgc ggatctgctc aggattacgg aggaactgtt
     1261 ctgtcagctt ctggatttcc ggaggcatcg tcgccgagaa gaacattgtc tgccgcgatg
     1321 gaggcagaag cttgcaaatg cgttcgatgt cgggaatgaa acccatgtcc agcatgcggt
     1381 ctgcttcgtc gatcacgagg taatcgaccg cattcatcag aagctttcct cttccgaagt
     1441 ggtcgagaag gcgacccggc gtcgcgatca gaatatctgc accgcgttcg agctttttct
     1501 cctgatcatc aaaagacaca ccgccgatca aaagcgccac cgtcaacttg tggtgcgatc
     1561 cgagcgcttc aatagcttcg gccacctggg ccgcgagctc gcgagtcggc tcgagaataa
     1621 gagagcgcgg catacgagcg cgggcgcggc ctttctcaag catcgtcaga aggggaagaa
     1681 cgaaggaagc cgttttgccc gttccggtct gagcgatgcc gacaacgtct cggccctcta
     1741 gggcaaatgg aatggcttgg gcttggatgg gggtgggggc ggtatagcct gccgcctcga
     1801 tggctgccag cactttttcg ctgagcttca gattttgaaa agtcatatcc cgttgaattt
     1861 ttggaaccac gtccttgggg ttcggtgagt atcaccatac gcacattgcg aaatgatgac
     1921 aggagcgcgt caaaccttcc atctcgagtc gcattgcgga taggctgcga tactttacat
     1981 tcgagagtac tgccgaacat gcggcagaaa ccgagttctg tctggcgctt gcaagatata
     2041 tgatcggaac tgcatgttcc gtcaaccctg atcggtcaat ctgcgtgaaa ctgtctccaa
     2101 tattccagag tctttgcatg gaaagagcga ccaggaagca acggcgagct aaagggcggc
     2161 gtttagcgag gctggcccgc ttacaaactt gttccctttt aagtagaagc gaagaggcct
     2221 caccgcatcc ttggttatcc cgattcgcac gctgacgccg atggcgccga acgcttctcc
     2281 gtcagaggcg agccagagtg agcccgcggc gcacaagtcg gttccgtcaa ggctgcggtc
     2341 gatatcgaga gcttttgcca agcgtcccgg accgcgtaag agatcgcgtg ttggaacatc
     2401 gccgcggcgc tccctcatgg cttcaagccc ggtgacaggt tcagccgcgc ggatcaagac
     2461 agcctcccct gcgcctgccg ccgcgctcga cacgttcagc atgaacgaaa cgccataggc
     2521 gagataaaca taagcaaatc ccggcgggcc gaacatgacc ttgttgcgcg gcgtggggcc
     2581 ccggaagccg tggcttgctt catctccggt aagataggcc tctgtttcga cgatgcgggc
     2641 tgtggcgagc gtgtccggaa acgcccggac aactagtttt ccgatcaaaa accgtgcgag
     2701 cgctgccgta tccatcgata gggcctcgcg gtcaagcggc tgtgggggga tgctcatctt
     2761 ggctccgcgg cagggttcga ccgcctcgga gattacaggc cctgcgcagt ttgggaagca
     2821 tcaacccgac tttccttcac gaacgggcgc tgctcgcggc tgattgcgga tttggtcgac
     2881 acgatgacta ttcttttcta ccttaggttg cgataatcag gcataactga ggctgggccc
     2941 atctcagcgc agtaaccaac aatcatagcg gtccgcgcag accgaaagcg tctgctcatc
     3001 aagaggcccc tgggctaaat aactcgccaa aaaagacatg tacaaaaaaa taaaatgcat
     3061 ttttatacat taagtttgaa gcttattaca agtaggtgaa gccttagctt tgtgttatac
     3121 attacctagc gggattatgg caatccatac atcgaaccgt gaaatcgctg tcgtcatcac
     3181 cacccagttt tagcctccgt ccaacgacgt caaattatta ggatctacat aaaggataaa
     3241 gatacaagca ttaagaaggg aatatatttg tccggcctcg cttgaagcga agcgttagtt
     3301 gaatcaagcg ttttgtcccc gggatcgtca atgcttaaag aagaccaaat tgcgctcgac
     3361 gatccacgtt gcgataaaat attggcaacg ggacaaacca tcaaacgacg catcttccta
     3421 ttcagtgcgt ttataatagt ttttatttgc attgtagccg tcgctctcgt tgccgagcaa
     3481 cggcaatcag ccctcgatcg tgcctccgcg cattcggcga acttgtccgc cgcattcgag
     3541 gagcaggtcc ggcgcgtcct ggatagcgtc tcgggagcga tggatctgat cgtgagccgg
     3601 atcgaaaaag aggggtcgga tttcgacctt tcgcaatggg cgccgcttat accgggagtt
     3661 gcgatctcta caatacaggt tgctattatt ggaccggatg gaatgctgtc ggcgtcttcc
     3721 ctttcgaaga acccgaagcc ggtcgatctt tcagaccgcg aacactttcg cgttcaccgc
     3781 gacaatccaa atctcggcct gttcatcggg aaacccgtgc tggggcgcgt gtccaaccag
     3841 atcaccatcc aggttacaaa acgcttacaa gcccgcgatg gatctttcgg cggcgtcctc
     3901 gtttttgcgt tgaacccgga ttatctaaca accctccacc gccaggtcga cctcggccaa
     3961 gctggaaacg tcaccgtttt cggcttggat tctgtcatcc gtgcgcgttt tacatccagc
     4021 aatagctcgg aggcaccggg cgttggttct tctatggccg ccggaaggtc cttcaaggat
     4081 gcagctttcg ctgacgcggg gacccacacg agcgccagcg ttatcgacgg ggtggtgcgg
     4141 atcttcaatt ggcgcaaggt cgccggctat cccctgatcg tgactgtcgg ccttgggaaa
     4201 gcggaagcgc tgagcgcagc agatcaccag gcgctgctca ttctggggat cgccgcgtcc
     4261 gcagtccttc ttgtcggcct catggccgtt aggctttccc gcgagatttc caacagggtt
     4321 acgcatgaga tcgctctcta ttatgaaggc gagagattga gagcggctca tgactgcctg
     4381 acggaccagc acgaaaggct gattgcgaaa agcgcgctgc ttgccgagga gcggatcaag
     4441 ctgcaagaga ccaacgcgca actttccttg gctcagcagc gttccgaggc ggcgagcaag
     4501 gcgaaatccg cgttcctggc gaatatgagc catgaactgc gcacgccgct
//
LOCUS       DATMGX010000198         2424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_301874, whole genome shotgun sequence.
ACCESSION   DATMGX010000198 DATMGX010000000
VERSION     DATMGX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301874"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VNR65_03215"
     CDS             complement(<1..516)
                     /locus_tag="VNR65_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17718.1"
                     /translation="MSQQIDTRSGIKIEVDRTVSNDQVQLSLHARTPKEVVLYWTLLR
                     RPEDAGQIPPQSYWPEGTSASGTSAAQTRFAGQNGERRVTLRFDHPSDFSLLDFALYF
                     PQENRWDNNQNRNYRIALAASPAGASGASPAEVLQRELAGQEVPFQHVYDIEEGRLAV
                     AVLHADDRYRLT"
     gene            complement(951..>2424)
                     /gene="glgA"
                     /locus_tag="VNR65_03220"
     CDS             complement(951..>2424)
                     /gene="glgA"
                     /locus_tag="VNR65_03220"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HWJ17719.1"
                     /translation="ARLRIVMVAPEAVPFAKTGGLADMVSSLSLQLERAGHEVTLILP
                     AHASALRGSVPLEELPGRLPVSMMVGAPSPAILRGRIGDAVTVYLVHAEGVFDRPQLY
                     GTTDGDYPDNAERFVFFCRAALAVMHRLDPPHILHAHDWQAAMAVAFLKAQPWAYPKL
                     EGTRTVFTVHNAGYQGIFDASKWPLLHLNPEFFTSRYLEFYGRINFLKAGLMFADAIT
                     TVSPSYAREVQSVEQGFGLEGVFRQRAARLVGILNGADYEVWNPTNDPYVVSPYSAED
                     LAGKRACKADLQRNFGLPENPDVPLLGMVARLVDQKGCDILANALPELFQRDVQLVVL
                     GTGDRYYQELFRALPARYPGKAGVRIAFAEELAHKIEAGADMFLMPSRYEPGGLNQLY
                     SLHYGTIPIVRAIGGLQDSVREFDPKSSTGTGFLFESYDGAALLAAVDRSLAAFAEKT
                     AWSSLMQNAMHADFGWAAATDRYLELYREILLEADRVSGS"
BASE COUNT          485 a          736 c          753 g          450 t
ORIGIN      
        1 cgtcagccgg taacggtcat cagcatgcag aacggctacg gcaagccggc cctcttcgat
       61 gtcgtagacg tgctggaacg ggacttcctg gccggcaagc tcgcgctgca acacttccgc
      121 tggggacgcg ccggatgccc cggcgggcga tgcggccagc gctatgcggt agttacggtt
      181 ctggttgtta tcccagcgat tctcttgcgg gaaatacaag gcgaagtcca gcagagaaaa
      241 atcggacgga tgatcgaacc gcaaggtaac gcggcgttcg ccattctgtc cggcaaagcg
      301 ggtctgcgcg gccgacgtgc ccgaagccga agtgccttca ggccaatagg actgcggtgg
      361 aatctgcccc gcgtcctcgg gcctgcgcag cagggtccag tacagcacta cttccttggg
      421 agtccgcgcg tgcaacgaca attgcacctg atcgttgctg acggtccggt caacctcgat
      481 cttgatgccg ctccgggtgt ctatctgttg gctcatagtt cgattaaaag ttcgattaag
      541 agttcgatca aaacgctttc gctaccgcaa tcgatgcgca gccctctggc ctgtcagcgg
      601 tgcggcaaac gcgctgccac cgcttcggag cgatggcccg tcatgccgcc tctgcttcat
      661 tgaagcgctc gattggccga tgaaacaggc aactggtcct gccagttgga taaacgtgca
      721 gaaagtatcg ctttcgtatc gacggcgcat ttccctctgt cattagatgc caaattctac
      781 gataagaatt gggacctgta ggcaacaagc aattatggca actgggcaat cggttgagat
      841 aggccggatt tgggaattgc ttgggatgct cctgtcggtc ttcgctgttc actccacttt
      901 cgttgaagag aaactctgcg agcgggcggc cgctggcgcg ggtttgcgga tcaggatccg
      961 gaaacccgat ccgcctccag cagaatctcg cgatagagct ccaagtagcg atccgtggcg
     1021 gccgcccagc cgaagtcggc gtgcatcgcg ttctgcatca acgaactcca cgccgtcttc
     1081 tccgcgaagg cggccagcga gcgatcgact gccgcaagca gcgctgcgcc atcgtacgac
     1141 tcgaagagga acccggtgcc agtgctggac ttgggatcga attcgcgtac gctgtcctgc
     1201 aagccgccaa tggcgcgaac gattgggatg gtgccgtagt gcaagctgta gagttgattc
     1261 aatccaccag gctcgtagcg cgagggcata agaaacatgt cggcgcccgc ctcgatcttg
     1321 tgggccagtt cctccgcgaa ggcaatgcgg acaccggcct tgcccggata gcgtgccggc
     1381 aaggcgcgaa atagctcctg ataatagcga tccccagtgc cgagaacgac gagttggacg
     1441 tcccgctgga acagctccgg cagagcgttc gccagaatat cgcaaccctt ctgatcgacc
     1501 agacgcgcca ccatgccgag caacggtaca tccgggttct cgggcaggcc gaagttgcgc
     1561 tgcaaatcgg ccttgcaagc gcgctttccc gcgaggtcct cggcgctgta agggctcacc
     1621 acgtaggggt cgttggtggg attccagact tcgtagtcgg cgccgttcag gattcccacc
     1681 aggcgcgccg ccctctgccg gaagacgccc tcgaggccga agccctgctc cacgctctgc
     1741 acctcgcggg cgtagcttgg gctgacggtg gtgatggcat cggcgaacat cagtccggcc
     1801 ttgaggaagt tgatacgccc gtagaactca agataacggg aagtgaagaa ctcggggttg
     1861 agatgcaaca gaggccactt ggaggcgtcg aagattccct ggtatccggc gttgtgaacg
     1921 gtgaagaccg tccgcgtccc ttcgagcttc ggataagccc agggctgcgc cttcaggaac
     1981 gcaaccgcca tggcggcctg ccagtcatgc gcgtgcagaa tgtgcggcgg atcgagacgg
     2041 tgcatcacgg ccagagcggc acggcagaag aagacgaaac gctcggcgtt gtcagggtag
     2101 tcgccgtcgg ttgttccgta gagctgcggc cggtcgaaca cgccctcggc gtgcaccaga
     2161 tacaccgtga ccgcgtcgcc aattctgccc ctcagaattg ccggactggg agcgccaacc
     2221 atcatggaga ccggtagacg ccccggcagt tcctccagcg gcaccgagcc gcgcaaagcg
     2281 gaagcatgcg ccggcaggat cagcgtgacc tcatgccctg cccgctccag ttgcagcgac
     2341 aacgaactca ccatgtcggc cagtccgccg gtcttggcga agggcacggc ttccggcgcg
     2401 accatcacga tgcgcaggcg cgcg
//
LOCUS       DATMGX010000199         4296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_201841, whole genome shotgun sequence.
ACCESSION   DATMGX010000199 DATMGX010000000
VERSION     DATMGX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201841"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VNR65_03225"
     CDS             complement(<1..212)
                     /locus_tag="VNR65_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWJ17720.1"
                     /translation="MLLSTKGRYAVMAIVEVARQSDARPISLGDIASRLELSLAYLEQ
                     LFMKLRRKGLVRSVRGPGGGYVLGRDA"
     gene            523..1215
                     /locus_tag="VNR65_03230"
     CDS             523..1215
                     /locus_tag="VNR65_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ17721.1"
                     /translation="MPEVIINGPAGRIEGRYHHEPTPGAPIAIILHPHPQFGGTMNNQ
                     IVYSLYYTYVQRGFSVLRFNFRGVGRSQGLFDQGPGELSDAATALDWLQLANPDAKSC
                     WIAGVSFGSWIAMQLLMRRPEIDGFIAVAPPANLYDFSFLAPCPSSGLMINGDRDRVV
                     PTNSVKTLVDKLKTQKGIVVSHEIVPGANHFFEDKIDDLIGVVEKYLDKRSTVTPKPT
                     RKEKVPQETELK"
     gene            1477..1854
                     /locus_tag="VNR65_03235"
     CDS             1477..1854
                     /locus_tag="VNR65_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ17722.1"
                     /translation="MGSTILIVEDNELNMKLFHDLLEAHGYKTLQTRNGIDAIALARK
                     HRPDLIVMDIQLPEVSGLDVTKWLKEDDSLRHIPIVAVTAFAMKGDEERIREGGCEAY
                     VSKPISVSRFLETIRQFIPPTLS"
     gene            complement(2362..2529)
                     /gene="rpmG"
                     /locus_tag="VNR65_03240"
     CDS             complement(2362..2529)
                     /gene="rpmG"
                     /locus_tag="VNR65_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWJ17723.1"
                     /translation="MAKPVTQKIKLLSSAGTGYFYVTKKNPRNLTEKLTLKKYDPVVK
                     KHVDFKEAKIK"
     gene            complement(2661..3128)
                     /locus_tag="VNR65_03245"
     CDS             complement(2661..3128)
                     /locus_tag="VNR65_03245"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HWJ17724.1"
                     /translation="MQSSFGNEHSPHPGQVWTSAHSLADRRPVLKSILRGASLRCPKC
                     GRRTLFSSYLKTSRICASCGEELFHHRADDAPPYFTIFIVGHIIVSLLITVELLYEPP
                     LWLHFLMWLPLTSLLSLALLPVVKGAIIGLQWALRMHGFEESGGPNKASSLSG"
     gene            complement(3143..>4296)
                     /locus_tag="VNR65_03250"
     CDS             complement(3143..>4296)
                     /locus_tag="VNR65_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HWJ17725.1"
                     /translation="SHRFVRGMMRSAAKLSYQQAQAAIDGKPNAKTAPLLEPILKPLW
                     QAYSILKEARDRRGPLDLDLPERRIILDERGLVSRVIVPERLEAHRLIEEFMIQANVA
                     AAEALESKKSPLIYRVHDAPSAEKLVALSEFLASIDINAPKTSFNRPGQFNKILAKAV
                     GTEHEEILNEVVLRAQAQAEYRPGNLGHFGLNLRRYAHFTSPIRRYADLIVHRALVSA
                     FGLGEGGLSDAEKTKLDAIAEAISQSERKAMSAERETVDRLIAMHLAEKVGEQFKARI
                     AGVTRFGLFVKLLDTGADGFVPIATLGRDYYKHVENHHALVGERSGESFRLGDRVDVK
                     LVEANPAAGALRFEMLSDGRYSPALAKAGRKRGGNGARKTAKGPKRRSK"
BASE COUNT          938 a         1236 c         1190 g          932 t
ORIGIN      
        1 gcatcgcggc caagcacgta tccgccgccc ggtcccctga cagacctgac gaggccttta
       61 cgtcgcagct tcatgaaaag ctgctcaaga taggcgagcg acaattccag gcggcttgca
      121 atgtcgccaa gcgagattgg ccgcgcgtcg ctttgccttg caacttcgac aatcgccatg
      181 acggcgtacc ggcctttcgt actaagcaac attttcttcc ttcttcgcgc cgaatttgcg
      241 cgaaggccgt ggaaatttac gcctctgtta tgatatgcca cgtcagacgc tgctccaggg
      301 cgcgcgacgg actcgaacac gtcgaggatt cttcactctg tgtataggat ttccgactgc
      361 aggagtcaac tattgactgc gccctcctga ctgaatttgt tccaaaaggg aggccttaac
      421 ggctctgctc aagggccatc cgctctttca agaagcggcc ggaacatgca gttagatgag
      481 taagcaagga gccggatttt catccggaaa agaggttcaa gaatgcccga agtcatcatc
      541 aatgggccgg ccggccgcat cgaaggacgc tatcatcatg agcccacacc cggtgcgccc
      601 atagccatta ttcttcatcc ccacccccag ttcggtggga cgatgaacaa tcagatcgtt
      661 tacagccttt attataccta tgtgcagcgc ggcttttctg tcctgcgctt caatttccgg
      721 ggtgtcgggc gcagccaggg tcttttcgac caagggccgg gggagctttc ggatgccgcc
      781 acggctctcg attggctgca actcgccaac cctgacgcaa aatcatgctg gatcgcggga
      841 gtgtccttcg gctcctggat cgccatgcaa ctcttgatgc ggcgaccgga gatcgatggc
      901 tttatcgcag tcgctccgcc cgctaatctc tacgacttca gctttcttgc tccctgcccc
      961 tcttcgggcc tgatgatcaa cggtgatcgc gaccgggttg tgcctacaaa ctctgttaag
     1021 accctggtgg acaagctcaa gacgcaaaaa ggcatcgttg tgagccacga aattgtgccg
     1081 ggcgctaacc atttcttcga ggacaagatt gacgacttga tcggagtagt ggaaaaatat
     1141 ctagataagc ggtccacggt cacgccgaag ccgacgcgca aagaaaaggt gccgcaggaa
     1201 accgagttga aatgaccgcc catgacaggc attcggcggc tgcgggctta acacaagctt
     1261 aagctgccga gatgactgga ataacgccag tggttgtgtt aatcaaatta cacgcggtga
     1321 ggatttatta accggttcac gagaatatga tggccaggat cgttcttttg acagtggccc
     1381 atgcgcctga actcttattc aaaacgcggt atgagaccgc aaaggcagat ggcaacgcca
     1441 tctaagagcc tgcggccaaa atcgagtaaa agcgctatgg gtagcaccat tctgattgtc
     1501 gaggataatg agctcaacat gaagctcttc cacgatctgc ttgaggcgca tggctataaa
     1561 acactgcaaa cacggaacgg catcgacgcc atcgcgctcg ctcggaagca tcggccagat
     1621 ttgatcgtca tggatatcca gctcccggaa gtgtccggtc tggatgtcac aaaatggctg
     1681 aaggaagacg atagcttgcg tcacatcccg attgtggcgg ttaccgcctt tgcgatgaag
     1741 ggcgatgagg agcgtatacg ggagggtggc tgtgaagctt acgtctcaaa gcccatctct
     1801 gtgtcgcgat tccttgaaac gatacgccag ttcatcccac ccacgctttc ctaggcgctg
     1861 gggatatcgg ggaaaactca aaaaccgcgc taccctttat aaaagggcaa tgctttgcgt
     1921 gtatcgttta cgataaaagc agaaagcgtt gataaataag tgcttgaggg atatcctgaa
     1981 aaaagcggaa cggaattttg cgtgttgaag ttggcggcgt gttaaggagg attggcgcaa
     2041 aatccgcgaa ttaaggcagg aagatggcaa tccggccttg ccaaacggca tttttttctg
     2101 atacttggga ttaaaggata ccctgcggag tgctcaggtc cgccgcatct cctgggtccg
     2161 tagggaatag accgaagcct ctctgtcgtc gcaaactgga gggcttcgga agatgctgtt
     2221 ggaccacgcc ctcttggttc aatggcgcta agatcagctc cgcatctcca atcggatcta
     2281 ccccgctgcc gataggtgcg gagctgatca tatttccgat tgggagggag cgagcgcccg
     2341 cgggtgctgt gacccgccag cctacttaat tttagcttct ttgaagtcca cgtgcttttt
     2401 caccacggga tcgtacttct tcagcgtgag cttttccgtc aaattccgcg ggttcttttt
     2461 tgtgacgtaa aagtaacccg tcccggcgga gctcaacaat ttgattttct gtgttactgg
     2521 cttagccatg gtaggttcca aatatctcca acgcaggctg caaagtggcc aacttgccgt
     2581 actatgtcaa gccgatgacg agcataattt cacttcagtt gaaaagtcgc gcacaacggt
     2641 cccgttgcgt gtccgaattg ctagcctgaa aggctcgaag ccttgttcgg gccgccactc
     2701 tcctcgaacc cgtgcattct gagcgcccat tgaagcccga ttatcgcgcc cttcacaacc
     2761 ggcagcaggg caaggctcaa aagggacgtg agcggaagcc acatcaggaa gtgcagccac
     2821 aaaggcggct cataaagaag ctctaccgtt atgagtagcg acacaatgat gtggccaaca
     2881 ataaagatcg tgaaataggg cggcgcgtca tcggcccgat gatgaaacag ctcctcgccg
     2941 cacgacgcgc aaatccgcga cgttttcaga taggacgaaa acagcgtcct tcgcccgcat
     3001 ttggggcatc tcaaagaggc cccgcgcagg atggatttca gaacgggcct gcgatctgca
     3061 agactatgcg cgctggtcca aacctgaccg ggatggggag agtgctcgtt cccgaaactg
     3121 gactgcactc gatctactct ttctatttcg aacgcctttt gggccccttg gctgtcttcc
     3181 gcgccccgtt cccgccgcgc ttcctcccag cctttgcgag tgccggcgaa taccggccgt
     3241 cgctcagcat ctcgaaccga agcgcgcccg cagcggggtt ggcttcgaca agcttcacat
     3301 ccacgcggtc cccgaggcgg aagctttcgc cggagcgctc cccaacaagc gcatgatggt
     3361 tttcgacgtg cttataataa tcccgcccaa gcgtcgcgat cggcacaaac ccgtccgcac
     3421 ccgtgtcgag caacttcacg aacaacccga accgcgtgac ccccgcaatg cgggccttga
     3481 actgctcgcc gaccttttcg gccagatgca tcgcgatgag cctgtccacg gtctcccgct
     3541 cagcggacat cgcctttcgt tcgctctgcg agatcgcctc ggcaatggca tcgagcttcg
     3601 tcttctccgc gtccgacagg ccgccctcgc caagcccgaa agcgctcacg agcgcccggt
     3661 ggacgatgag atccgcatag cgccggatcg gcgaggtgaa atgcgcgtag cggcgcagat
     3721 tgaggccgaa atggcccaga ttgccgggcc ggtactcggc ctgtgcctgg gcgcgcaaca
     3781 caacctcgtt caggatttct tcatgctccg tgccgaccgc tttcgccagg atcttgttga
     3841 actggccggg acgattgaag ctcgtcttgg gagcgttgat gtcgatgctg gccaggaact
     3901 cgctgagcgc cacgagcttt tccgccgagg gcgcgtcgtg caccctgtag atcaggggcg
     3961 actttttcga ttcaagcgct tccgccgccg cgacgttcgc ctggatcatg aattcctcga
     4021 tgagccggtg cgcctcaagt cgctcgggca cgatcacgcg cgatacaagc ccgcgctcgt
     4081 cgaggatgat tctgcgctcg ggcagatcga gatcgagtgg cccgcgccgg tcgcgcgcct
     4141 ccttgagtat cgaataggcc tgccaaagcg gcttcaggat aggctcgagc agcggcgcag
     4201 tcttcgcgtt cggcttccca tcgatggcgg cctgtgcttg ctgataggaa agcttcgcgg
     4261 cagaccgcat catcccgcgc acaaagcgat gggatt
//
LOCUS       DATMGX010000200         3126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_426434, whole genome shotgun sequence.
ACCESSION   DATMGX010000200 DATMGX010000000
VERSION     DATMGX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426434"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..487)
                     /locus_tag="VNR65_03255"
     CDS             complement(254..487)
                     /locus_tag="VNR65_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17726.1"
                     /translation="MPFYFFRIQNGPYSGGSDHGTELADRIAAWKELTSVCGDIVGSA
                     SSKLQQNSEWQMELLDDTKRPLFRIRLIAESLE"
     gene            complement(659..853)
                     /locus_tag="VNR65_03260"
     CDS             complement(659..853)
                     /locus_tag="VNR65_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17727.1"
                     /translation="MTVHAPKLFTPEEFASLIEVAVSSPNPTIPASHLTKLISLGYVV
                     VTAKGPVVTGDGLIRITESE"
     gene            complement(1233..1436)
                     /locus_tag="VNR65_03265"
     CDS             complement(1233..1436)
                     /locus_tag="VNR65_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17728.1"
                     /translation="MVHKGVEYMVAPTATPGIWRWQFQIGEQVKTGRTETRINLLAAR
                     RVQLRIDRELKKVEPQPTPEFKS"
     gene            complement(1602..1778)
                     /locus_tag="VNR65_03270"
     CDS             complement(1602..1778)
                     /locus_tag="VNR65_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17729.1"
                     /translation="MDSFIHRENLALYRRRLVEAKDDATRKVIQQLLTDEEAKDNIPR
                     VSQGGGSAGKPNAS"
     gene            2550..>3126
                     /gene="recA"
                     /locus_tag="VNR65_03275"
     CDS             2550..>3126
                     /gene="recA"
                     /locus_tag="VNR65_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWJ17730.1"
                     /translation="MSATALRIVEGSSMDKTKALSAALSQIERQFGKGSVMKLGKNDR
                     SMDIETISSGSLGLDIALGVGGLPKGRVVEIYGPESSGKTTLALHTVAEAQKKGGICA
                     FIDAEHALDPVYARKLGVNIDELLISQPDTGEQALEICDTLVRSGAVDVLVIDSVAAL
                     VPRAELEGEMGDALPGLQARLMSQALRKLTAS"
BASE COUNT          649 a          914 c          946 g          617 t
ORIGIN      
        1 agtttgccgc tcaatcgatt gatgaagtga agccggttgc acgcctggca gactacggtt
       61 tcgtaggctg aggacggatt gtccggctca gcctcgtcca gccaatgctg cacgttcaga
      121 ccggtttgcg gacatttgaa gatgacatga cccatgaata gggaatttat gacagctccc
      181 gggcacttcc gtcgttgatc tctgtcaatg tccggggcag caagatatga aagtcgggtt
      241 cccgccccat gggctattcg agcgattccg cgatgaggcg gatgcgaaac agcggccttt
      301 tggtgtcgtc gagaagctcc atctgccatt cggagttttg ctggagcttg ctggaagcgc
      361 tgccgacgat atcgccgcaa acggaggtca gctctttcca ggcggcaatg cggtcggcaa
      421 gttcggtgcc gtgatcggac cccccggaat aggggccgtt ctggatgcga aagaaataga
      481 acggcatgac acagcaccga cgcgtgtggg ccgctctggg ctgttgaaaa acccacgagt
      541 tgctgcgtga gtggattccc taagccggcg cggatcaatt ccggcatctt acgggcttaa
      601 gtacggtatc gaccactctc ttgggcggct cctgatcttg gcctcggttt gtcagcgctc
      661 actcgctctc ggtaatccgg atcagaccat cgccggtgac gacgggccct ttggcggtca
      721 cgaccacgta gccaagggaa atcagcttgg tcaaatggga cgccgggatc gtgggattcg
      781 gcgaactaac cgcgacctcg atcagcgagg cgaactcttc tggcgtaaag agctttggcg
      841 cgtggacggt cattgctgaa ctctggttgc gtgttcgggc agccggggcc agtaacatcg
      901 aaatgaaagc ggccgcaacg gggcggcctc atgaaagagc cccgaaccaa ctaaagtccg
      961 gggctcgatg tcaacgtcgc agcttccccc gtgggcagtt cggcaatgac aaattgaatt
     1021 ctagggccgt cggcagaaac cgccttgcgt cagatcaaaa tggcgtcaga tatcagatca
     1081 agaccacaaa ttcagcgatc agcgagcgac ataagtaggc aaagggcaag accagcgtct
     1141 ccgcgacaag tcgaatacgg aactgcggcg cgctggcgct ctcgcgaagt tccatgcacc
     1201 gccatcgccg acgtggtcac cccagaccat ggtcagctct tgaattccgg cgttggctgc
     1261 ggttcgactt tcttaagttc acggtcgatg cgcagctgaa cccggcgcgc ggccaggagg
     1321 ttgatcctgg tttcggtcct gccggtcttg acctgttcgc cgatctgaaa ctgccatcgc
     1381 cagattcccg gggttgccgt aggggcaacc atgtattcca cgcctttgtg caccatgagg
     1441 acttctcccg tcgcgactcg aagccgaccg atcctaacca ctaaccgaag ttgcacctgt
     1501 tcaattgtgc tcgtctttgg aaagtggcag atcatggggg cgggcttgcg tcgggtgacg
     1561 cgggtcaaag ggatcttgcg ctgttcgcga gggcggcggg attaggaagc attgggcttc
     1621 cctgccgagc cgcctccctg gctgacccga gggatgttgt cctttgcctc ttcgtccgtc
     1681 agcagctgct gaatcacctt gcgcgtggcg tcgtctttgg cttcgacgag ccgtctcctg
     1741 tagagggcca gattttcgcg atggatgaag ctatccatag cgtcctcctt tcaagggccg
     1801 ccgatgtttg caaaccggac actactcctc gaagggaagc cgctccacgg aaaagaaatt
     1861 caccgccgtc caggcggtgc caaggcagga cgccaatatc gagcgatagg gaactccaag
     1921 aatgaaagag gccgccaatc gggcgccctc ttggagggga ggtacgaagc gcctgggagt
     1981 ccggaccacc cgcccgcaaa aacggcatcc cagcgtggat gagctgcgat cgttcggcgt
     2041 gtagcttgtc taaccgagtt ggctggaagc ggcgttaatt tggcacaagc gctacggcgg
     2101 cccgttccac tcgccggtcg gcgagatggc cgggagctca aactcctgga atgcgacgac
     2161 gacgaccgcc tcgcattcct cgcaaaggaa aaatcctgat tcggtctcga ccctcgcgcg
     2221 cgcgttttgg caggcgtcgc agaccttcca ccgcgtccac atcggtcaat taggccataa
     2281 gcgcttaaaa cggaccagtc ccaaaatcta ggtatttata cggagaacgt tttaggggtg
     2341 tgttaacttt ttacacaacc tacagaatgg aacccgttcg gcaaattagg aaagcgccag
     2401 cacgatttag gacgcgtctg gtcaaatacg gacagcggct gctatccgaa aagaacatat
     2461 tgcgaacttg gaacaaatag ggtacattgt tcccgtcacc gccgcccgtt tgtcccgcta
     2521 gcgaatcccc gccataagga gcacgaccaa tgtctgccac tgccctgcgt atcgtcgaag
     2581 gatcctccat ggacaagacc aaggccctgt ccgccgcgct ttcccagatc gagcgccagt
     2641 tcggcaaggg ctcggtgatg aagctgggca agaacgaccg ctcgatggac atcgagacca
     2701 tttcctcggg ctcgctcggg ctcgacatcg cgctcggcgt cggcggcctg ccaaaggggc
     2761 gcgtggtcga gatctacggg ccggaatcct cgggcaagac cacgctggcg ctgcacaccg
     2821 tcgccgaggc ccagaagaag ggcggcatct gcgccttcat cgacgccgaa cacgcgctcg
     2881 acccggttta cgcccgcaag ctcggcgtca atatcgacga actcctgatc tcgcagcccg
     2941 acaccggcga gcaggcgctg gaaatctgcg acaccctggt acgatcaggc gcggtcgacg
     3001 tgctggtgat cgattcggtg gcagcgttag tgccgcgcgc cgaacttgag ggcgaaatgg
     3061 gcgacgcgct gccgggcctg caggcccgcc tgatgagcca ggccttgcgc aagctgacgg
     3121 cgtcga
//
LOCUS       DATMGX010000201         2218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_25169, whole genome shotgun sequence.
ACCESSION   DATMGX010000201 DATMGX010000000
VERSION     DATMGX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25169"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..2192
                     /locus_tag="VNR65_03280"
     CDS             78..2192
                     /locus_tag="VNR65_03280"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HWJ17731.1"
                     /translation="MSKTKTTSNSGKRKQKKGSVPNGDSQPVAGGELHQIAGGGHPAL
                     TTNQGVALSDNQNSLRANPHGPTLLEDFILREKITHFDHERIPERIVHARATGAHGFF
                     ELTASLKRYTTARILTEVGEKTPLFTRISTVAGGAGSVDTPRDIRGFAVKFYTKEGNW
                     DLVGNNIPVFFIQDAIKFPDLIHAVKMEPDRGFPQSATAHDTFWDFISLTPESMHMVM
                     WIMSDRTLPRSLRMIEGFGIHSFRLINHAGESTFVKFHWRPKLGLQSTIWDETVKICG
                     ADQDFHRRDMFDAIAAGNFPEWEFAVQLFTQAEADRFPFDHLDATKLIPEELVPLKVI
                     GRMVLDRWPDNFFAETEQVAFCPSHVVPGIDFSNDPLLQGRLFSYHDTQLSRLGSPNF
                     HQIPINAPKCPFANQQRDGHMQMEQPAGRVAYEPNSLSSNSPRETPAKGFHSAGVTET
                     GEKGRIRAESFADHYSQARQFYLSQSAYEQAHIASALVFELSKVEHPHIRLAMVGHLR
                     HIEEDLAKRVATGLALSKLPDAPIAAAPVRQMKPSPALQLIGKMKATLEGRAIGILIA
                     DGSDGRIIKKIKKAATDAEASVKIIAPKVGGAKLADGSVLAADGQLAGTPSVLFDAVA
                     VILSDEGTKTLAMESAAIDFVRDAFGHLKAIAVDKGGRALLDIANVGQDAGVVDTDDK
                     DAFIAAAKTRQWDREKSIRTLA"
BASE COUNT          514 a          672 c          584 g          448 t
ORIGIN      
        1 attttctcgg aactggcggt ctcgcgagcg ccgctcgacg tacccccggc tggcactagt
       61 cactaaggag atcgatcatg agcaaaacaa agacgacgag caattctgga aaacgcaaac
      121 agaaaaaggg ttcggttccc aacggagact cacaaccggt tgcaggcggt gaactgcacc
      181 agatcgcagg tggaggccat cccgcgctca ccacgaacca aggcgttgcg ctctccgata
      241 atcaaaactc gctccgggcc aatccgcacg gccctacgct actggaggat ttcattctgc
      301 gtgaaaaaat cacgcatttc gaccacgagc gcattcccga gcgtatcgtt cacgcgcgcg
      361 cgacgggcgc gcatggattc tttgagctga ccgcctcgtt gaaacgatac accaccgcca
      421 ggatactcac cgaggtgggt gagaagacgc ccctgtttac tcgcatctca accgtcgccg
      481 ggggcgcggg ttcggtggat accccacgtg acatacgcgg atttgccgtc aagttttaca
      541 ccaaggaagg caactgggac ctggtcggca acaacatccc ggttttcttc atccaggatg
      601 ccatcaaatt ccccgacctc atccatgccg taaaaatgga acccgaccgc ggctttccgc
      661 aatcagcaac cgcgcacgac accttttggg atttcatttc gctgacgcct gaatccatgc
      721 atatggtcat gtggatcatg tccgatcgca ccttgccgcg ttcactgcgg atgatcgaag
      781 gctttggtat ccatagcttt cggctgatta accatgccgg cgaatcaacc ttcgtcaaat
      841 tccactggcg tcccaagctc ggcttgcaat ccaccatttg ggatgagacc gtcaagattt
      901 gcggcgctga tcaagacttc caccgtcgcg atatgttcga cgccatcgcg gcgggcaatt
      961 tccccgagtg ggaatttgcg gttcagctct tcacgcaggc agaagcagac cgatttccgt
     1021 tcgatcattt ggacgcgacc aagctcattc ccgaagaact ggtgccattg aaagtcatcg
     1081 gccgcatggt gctggatcgt tggccggaca acttcttcgc tgaaaccgaa caggttgctt
     1141 tctgtccttc ccacgtcgtg ccaggcatag atttttcgaa tgatccactc ctccagggcc
     1201 gtttattttc ctatcacgac acacagcttt cgcgcctggg ctcgcccaat tttcaccaga
     1261 tccccatcaa cgcacccaaa tgcccgtttg ccaaccaaca acgcgacggc cacatgcaga
     1321 tggagcaacc ggcgggccgt gtagcctacg aacccaactc cttgtcttca aactcgcctc
     1381 gcgaaacgcc cgcaaagggc tttcatagcg ctggcgtaac cgaaactggc gagaaaggtc
     1441 gtatccgcgc cgaaagcttt gccgaccact acagccaggc gcggcaattc tatctcagcc
     1501 aatccgctta tgaacaggcg cacatcgcct cggcgttagt gtttgaactt tccaaggtgg
     1561 aacatccaca cattcgtctg gcgatggtcg gtcatctgcg acatatcgaa gaagacctcg
     1621 ccaagcgcgt cgccacgggt cttgcgcttt ccaaacttcc cgacgcgccg atagccgcag
     1681 cccctgtcag gcagatgaag ccttcacccg cattgcagct catcggcaag atgaaagcca
     1741 cgctcgaagg gcgtgcgatt ggcatcctga tcgcggatgg gtccgacggc aggatcatca
     1801 agaaaatcaa gaaggccgcg accgatgcag aggccagcgt gaagatcatc gcccccaaag
     1861 tgggcggcgc gaaactcgct gacggctcgg tgctggcagc ggacggacaa ctggctggga
     1921 cgccctccgt gctgttcgac gctgtcgccg tcatcctctc tgacgaaggc acaaagacgc
     1981 tggcgatgga aagcgccgcg atcgatttcg tgcgcgatgc tttcggtcat ctcaaggcca
     2041 tcgccgtgga caaaggcggc cgggcccttt tggatatagc caatgtcggc caagacgcgg
     2101 gcgttgtgga taccgacgac aaagacgcat tcattgctgc ggcgaaaaca cggcaatggg
     2161 accgggaaaa gtcaattcgc acattggcat aattgaaagg taattatgaa aatcctta
//
LOCUS       DATMGX010000202         2566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_201808, whole genome shotgun sequence.
ACCESSION   DATMGX010000202 DATMGX010000000
VERSION     DATMGX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201808"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VNR65_03285"
     CDS             complement(<1..222)
                     /locus_tag="VNR65_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17732.1"
                     /translation="MGRQKKLKGVALACGITLSLAQQITAAAAFSTEPVAPPSASLQA
                     NPPSQFIQPGISGQVPEFDLNDPSLAGKSK"
     gene            complement(375..728)
                     /gene="cutA"
                     /locus_tag="VNR65_03290"
     CDS             complement(375..728)
                     /gene="cutA"
                     /locus_tag="VNR65_03290"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HWJ17733.1"
                     /translation="MDQDAEMIVLYTTFPTEKDAQKVGEELVTEKLAACVNIFPGMVS
                     IYRWEDAIEHGSEVVMLVKTRKELESQVFAALAAKHPYSVPALIAFEPSRVAASYLEW
                     LRSQTVSSDGNSGPK"
     gene            797..1606
                     /locus_tag="VNR65_03295"
     CDS             797..1606
                     /locus_tag="VNR65_03295"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003406.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HWJ17734.1"
                     /translation="MAEVSPAHTQTAVFADKVRLGFVASPAPEAQAALKRLTKRYGAA
                     DPASASVIIALGGDGLMLQTLHRFMGMEVPIYGMHRGSVGFLMNDFKEDDLVERVSNA
                     KLSLIHPLKMQAENVAGEKLNALAINEVHLFRQTAQSARLAVTVDGKERLTELICDGV
                     LVSTPAGSTAYNLSVGGPILPLKARLLALTPISPFRPRRWRGALLSNKAQVRISVLEA
                     DKRPVSAVADHAEFRDVLSVLIREERSIGLKLLFDPGHALEERILAEQFRF"
     gene            1679..>2566
                     /locus_tag="VNR65_03300"
     CDS             1679..>2566
                     /locus_tag="VNR65_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17735.1"
                     /translation="MRTIIAAILVSFTVCSFAFTCQAEEARAVVILDATAQMGGNLGQ
                     KRKLDLARTGISGAVSRMDPGAAFALWAFGSSPQKKCEDVNELVPLQAAGGALRTLEK
                     ALSTLQPKAARAPAMDALQAALKSVGARDGKAVSAILVAGTGDDCIGDICGSAGRLHG
                     LYPKAKLTVFGMSMGEQAVANFTCAAKAMGGAFIGVKSGADLERNLREALGSAQTQAG
                     QQKVAVVTPTSDAAGAADAEPPKAPAAAPATTPPPAEQPAEKEAEEKFAPPPQSEPNV
                     VLSASLVPGMPPLEAGVTWE"
BASE COUNT          492 a          749 c          783 g          542 t
ORIGIN      
        1 cttcgatttc ccagccaacg agggatcgtt aagatcaaac tcgggcacct gcccggatat
       61 gccgggctgg atgaactgcg agggtggatt cgcttgcagg ctcgccgacg gaggcgctac
      121 aggctccgtt gagaatgccg cggctgcggt gatctgctgg gcaagcgaca aggtgatgcc
      181 gcacgccagt gccacccctt tcaacttttt ctgcctgccc atgagtctct cccgccttat
      241 gtccctaaga tgattgcgaa cctctatgaa tgcaagtttg ttgattcacg gcttaagtgc
      301 ctcatatttt tgatgttttc cagagggggc tgttaatccg ttgcggaaag gcatcagtgc
      361 ggggtaagtc cgccttattt tggtcctgaa ttcccatcgc tagagactgt ttgactgcgc
      421 agccattcaa gataggaggc ggctactcgg ctcggctcga acgcgataag ggctggaacg
      481 ctataagggt gcttcgccgc cagggctgcg aaaacttgtg actcgagttc ctttcttgtc
      541 ttgacgagca ttaccacctc gcttccatgt tcgatggcgt cttcccatcg atagatggaa
      601 accataccgg gaaagatatt gacgcaggca gcgagctttt cggttaccaa ttcttcgccc
      661 actttctgag catccttttc ggtcgggaat gtggtataga gaacgatcat ctcggcatcc
      721 tggtccatgg tttccctcga atgtgcagca tctgctcgct gtccgatttg ccggacgtcc
      781 aagctcagga gttatcatgg ccgaggtgag cccggctcat actcagacgg ccgtttttgc
      841 tgataaggtt cgactgggct tcgttgcaag ccccgcaccg gaagcgcagg cggcattgaa
      901 gcggctgaca aagcggtatg gggcggccga ccccgcttcc gccagcgtga tcatagcgtt
      961 gggcggcgac ggcctcatgc tgcaaacgct tcatcgtttc atggggatgg aggttcccat
     1021 ttacggcatg caccggggct ccgtcggctt cctgatgaat gacttcaaag aggatgacct
     1081 cgtcgagcgc gtgtccaacg cgaagctgag cctcatccat ccgctgaaga tgcaggcgga
     1141 aaatgtggct ggcgaaaagc tgaatgcgct tgcgatcaac gaggtgcatc tattccgcca
     1201 gacggcgcaa agcgcgcggc tggcggttac cgttgacggg aaagagcggc ttaccgaact
     1261 gatttgcgac ggcgtccttg tctccacgcc tgcaggaagc actgcctata acctctcggt
     1321 cggggggccg atcctgccgc tcaaagcgcg tcttctcgcc ctgacgccga taagtccgtt
     1381 ccgccccagg cgctggcgcg gagcacttct gtcaaacaag gcgcaggtca ggatttcggt
     1441 tctggaagcc gataagcgcc cggtcagcgc cgtcgccgat catgccgagt tccgggatgt
     1501 gctctcggtt ctcattcggg aggaacgaag catcggtctc aaacttctgt tcgatccagg
     1561 tcacgcgctc gaagagcgta tcctcgcaga gcagttccgt ttttaggctc ctggttgacc
     1621 cggctggccg gtgcccctta aatccagtca ggttttacga ccgcaaaggt caacgaaaat
     1681 gcggacaatc attgcagcca ttctggtctc attcaccgtg tgcagctttg cattcacgtg
     1741 ccaagccgag gaagcccgcg ccgtcgtcat tctggacgcc accgcgcaaa tgggtgggaa
     1801 cctcggccaa aagcgcaagt tggatttggc aagaacaggc atcagcggcg ctgtgagccg
     1861 gatggatccc ggcgccgctt tcgcgctctg ggcgttcggc tcatctccgc agaagaagtg
     1921 cgaggacgtc aacgaactgg ttccgcttca ggccgcgggc ggcgctcttc gcacgttgga
     1981 gaaggcgctg agcacgcttc agccgaaagc ggcccgggcg ccggctatgg acgccttgca
     2041 ggcggcgctc aagtctgtcg gcgcgcggga cggaaaggca gtctcggcca ttcttgtcgc
     2101 cggaacagga gacgattgca tcggcgatat ttgcggctcc gcgggacggc ttcatggtct
     2161 ttaccccaag gccaaattga ctgtttttgg catgagtatg ggcgagcagg cagtcgctaa
     2221 cttcacctgt gcggccaagg cgatgggcgg cgccttcata ggcgtgaagt ccggtgctga
     2281 tctcgaacgc aacctgcggg aggcactggg gtccgcacag acccaggctg ggcagcaaaa
     2341 ggtggcggtg gtaacgccca cgagtgacgc cgccggtgcc gctgacgctg agccgcccaa
     2401 ggctcccgcc gccgccccgg caactacgcc gcctccggcg gagcaacccg cggaaaagga
     2461 ggcggaagag aaattcgctc cgcccccgca atccgaaccg aacgtcgttc tttcggcttc
     2521 tcttgtaccg ggcatgccgc cgctcgaggc gggcgtcact tgggag
//
LOCUS       DATMGX010000203         2314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_148700, whole genome shotgun sequence.
ACCESSION   DATMGX010000203 DATMGX010000000
VERSION     DATMGX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148700"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VNR65_03305"
     CDS             complement(<1..994)
                     /locus_tag="VNR65_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWJ17736.1"
                     /translation="MSSVHAEGKRGPGVVRAAVKISLGCVLLGGCSAGTERFTNLEFG
                     SGYEAAPPRSSTPASTAAINAPQYRPVREAASSQPKPQSGYQLASATPDQSGGYLQVS
                     RVDLPPVQQQSSGSGGVKTADGYGPYGPAPLSDGSYAGPRVYSPYGGPNGYAPPPPPA
                     GGFGPPPSGYSASPQGYYGGGSSYAPPPPQDPGSGPYGYEQQSQNQPKGRGYDRAPQG
                     YDRAPQDYAYYGPNQGNTAPVDGKRTGRPSGPAVADASRGKMVRLAPGETILSVAKRH
                     GVTVEAIARANGLRGRPVMPGTMLVIPAAPPPTQYGQARSAPRAKVAECAGGQCY"
     gene            complement(1093..1719)
                     /locus_tag="VNR65_03310"
     CDS             complement(1093..1719)
                     /locus_tag="VNR65_03310"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWJ17737.1"
                     /translation="MDEALRSLLYSLQHNGIGDPRVLSAIARIPRELFISEDALKAQA
                     YTDQALPIECDQTISQPYIVAYMSERLNVAADQDVLEIGTGSGYQTAILSQLARHVYT
                     MERHRELHELAVQRFERLGMHNITAIVGDGTEGWPDKGLFDRIIVTAGTRQVPDALLD
                     QLSPCGSMLLPLGPRYDQRITLITRMNEHLEYQTLIPVQFVPLVPRDH"
     gene            complement(1760..>2314)
                     /locus_tag="VNR65_03315"
     CDS             complement(1760..>2314)
                     /locus_tag="VNR65_03315"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106411 - XMP 5'-nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWJ17738.1"
                     /translation="CVIMGARYILQNELPTLILSGVNRGANLADDVTYSGTIAGAIEG
                     TMLGIPSIAMSLAIDWEDPKKIHWETPMELGADLVRKLMKAGWPRDVLMNVNFPDREP
                     GKVQGIQVTEQGRRDQDYLRIEDRMDTRGNPYYWLGFKPRSLHPGKGTDLHAVKSGAI
                     SVTPLYLNLTHSDTCRKLTGLLEA"
BASE COUNT          435 a          714 c          693 g          472 t
ORIGIN      
        1 tgtagcattg gccgcctgcg cattcagcga ccttcgccct gggcgcgctc cgtgcttgtc
       61 cgtactgcgt cggcggaggg gctgccggga ttacaagcat ggtgccgggc atgacggggc
      121 gaccacgaag cccgttcgcg cgcgcgatcg cctcgacggt cacgccgtgc cgcttggcca
      181 cagaaagaat agtttccccc ggcgcaagcc tgaccatctt tccgcgcgac gcatctgcaa
      241 cggcaggacc ggaaggcctt cccgttcttt tgccatccac gggcgctgtg ttcccctgat
      301 taggcccgta ataggcatag tcctgcggcg cccgatcgta gccttgcggc gcgcgatcat
      361 atccgcggcc cttcggctga ttttgcgact gctgctcata gccataaggc ccgcttcccg
      421 gatcctgtgg aggcgggggc gcgtaactcg agccgccacc ataatatccc tggggcgatg
      481 cgctataacc actggggggc ggaccgaagc ctccggccgg tggcggaggc ggtgcatagc
      541 catttggccc gccgtagggc gagtagacac gcggccccgc gtagctccca tccgaaagag
      601 gggcagggcc atagggcccg tagccgtcgg ccgtcttcac tccgcctgag cccgaagatt
      661 gctgctgaac agggggaagg tcgaccctag acacttgcag atatccgccc gactggtcgg
      721 gggtagcgct cgctagctga tagccgcttt gcggcttcgg ttgagagctc gccgcttctc
      781 gaacggggcg gtattgggga gcgttgatgg ctgccgttga cgccggcgtg gaagaacgag
      841 gaggcgccgc ctcatacccg cttccgaact caagattggt gaaccgttct gtcccggcgc
      901 tgcacccccc caaaagtacg caacccaaac tgatctttac agctgcgcgg acaactccgg
      961 gcccgcgctt gccctccgca tgcacactac tcatcgccgc acctcacttt gcaataaaag
     1021 tattaggggc ggaacgctta atattaactt aaacgcttcc tattttttca cgctgcgcgc
     1081 gcataggcct tgtcaatgat cgcggggaac gagcggcaca aactgaacgg ggattaaagt
     1141 ttggtattcc aaatgttcat tcatccgcgt gatgagcgtg atccgttgat cgtagcgggg
     1201 accgagggga aggagcatgg atccgcacgg cgaaagctga tcgagaagtg catcaggcac
     1261 ctgcctcgtg ccggccgtga caatgatgcg atcgaaaagc cctttgtcgg gccatccctc
     1321 ggtgccgtcg ccgacgatag ccgtaatatt gtgcatcccg agccgctcga accgctgcac
     1381 cgccagctcg tgcagttccc gatggcgctc catggtgtaa acgtgacgcg cgagctgtga
     1441 gaggatcgcg gtctggtaac ctgagcctgt tccgatttcg aggacatcct gatcggccgc
     1501 gacattcagc cgctcgctca tataggcaac gatgtagggc tgcgaaatcg tctgatcgca
     1561 ttcgatgggc agagcctggt ccgtataggc ctgcgccttg agggcatcct cggaaatgaa
     1621 aagctctcgc ggaattctgg cgatggcgct cagcacgcgg ggatctccga tcccgttgtg
     1681 ttgcaggctg tacaaaagac tgcgcagcgc ttcgtccatg cgcaccactc ccggagcggc
     1741 accatgcagc ccgctcctcc tacgcctcca gaagccctgt cagcttccgg cacgtatcac
     1801 tatgcgttaa gttcagataa agaggcgtta ccgatatagc gcccgatttg accgcgtgaa
     1861 gatcggtacc cttgccgggg tgcagcgatc gcggtttgaa cccgagccag tagtaggggt
     1921 tgccccgcgt atccatgcgg tcttcgatgc gcaagtaatc ctgatcgcgc ctgccttgct
     1981 ctgtcacctg aattccctga actttacctg gttccctgtc cgggaaattg acgttcatca
     2041 agacatcgcg cggccagcct gccttcataa gcttgcgaac gagatcggcg cccagttcca
     2101 tcggcgtttc ccaatggatc ttcttcgggt cttcccaatc gatggcaagg ctcatggcga
     2161 tggacggaat gcccagcatc gtgccctcga tcgcgcccgc tatcgtgccg gaataggtga
     2221 cgtcatcggc gaggttcgcc ccgcggttga cgcccgacag gataagcgtc gggagttcat
     2281 tctgaagaat gtagcgtgcg cccatgatca cgca
//
LOCUS       DATMGX010000204         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_416122, whole genome shotgun sequence.
ACCESSION   DATMGX010000204 DATMGX010000000
VERSION     DATMGX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416122"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="VNR65_03320"
     CDS             <1..1684
                     /locus_tag="VNR65_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17739.1"
                     /translation="LLSEIASRFPASNSQGATGPAAATAQDTKQVDQLARGLASGLAR
                     DKRLALDALDQAYLEANPQSLWPILDGLVAAYQDAARRDENLIAAYQWLLANQLELIR
                     YRTERGWTWADKMLDEYQDRISEIAKSDHLSPGDLFSLAAALTDAKIPVDPKVSEAFF
                     RVDPGTVRAEPSPELLRAEVQPLIDQLGEAPDPFVVIESLSETAGFMPAEFRAFLAHE
                     FALSPYPVLRDAVPLMLLDSEAVVRRAAAAVLEQTAAADTLSPAALRRTIILRNWIPE
                     DDRAGVDRAIRKARTKGVICAQWNAPPKLSILALPIDGSGANGLILTTKPGKTGLFGG
                     VLLKQNFGLRDIWCDRDKPRHEIGQAIEGLLEDTAAIEVERHYLDVALQHHLAIGLSN
                     GRLPTPALLAIAESVGGVEWRASRLDIDAEIARVYSELDPAKIAPADIAASLKRTGEW
                     LHQESFAESWFENDAEVSAMIDEMSGQDDDAITGAVLSELLEKRRGVWTERFLLMALW
                     ARAAKSKNEGRYWRDFLILAHELSAGRPLEDVPIMAAIAQRTVSAALSGTPF"
     gene            complement(1779..2060)
                     /locus_tag="VNR65_03325"
     CDS             complement(1779..2060)
                     /locus_tag="VNR65_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17740.1"
                     /translation="MIDAQEERLIEALTKVDAHGDVARAHAQDPMTAVLRQKAIQADL
                     MRWDDSRGHYVLTGTGRNRISLRRRAPGVVVRFKKRDDRLDGPPHRAAD"
     gene            2073..>2267
                     /locus_tag="VNR65_03330"
     CDS             2073..>2267
                     /locus_tag="VNR65_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17741.1"
                     /translation="MIARSDLGQDFGEIAARAQRAHPTSAHRLDRGLTAPLTRLHSPF
                     NMTCPVIEGMRERGFGRIVSI"
BASE COUNT          426 a          685 c          693 g          463 t
ORIGIN      
        1 tctgctgtcc gaaatcgcgt cccggtttcc agcatcgaat tcccagggag ctacgggtcc
       61 agccgctgcg acagcgcagg atacaaaaca ggtcgatcag cttgcacggg gtcttgcctc
      121 aggattggcg cgggacaagc ggttagcgtt ggacgcactg gaccaagcct atctggaagc
      181 caacccgcag agcctgtggc cgatcctcga tggcttggtg gcggcctacc aagatgccgc
      241 gcgccgggat gagaacctga tcgccgccta ccagtggctg ctcgccaacc aactcgagct
      301 gattcgctat cggaccgagc ggggatggac atgggccgac aaaatgctcg acgaatatca
      361 agaccgtatc agcgaaatcg ccaagagcga tcatctctct cccggtgatc tgttctcgct
      421 ggcggcggcc ttgactgacg ccaaaattcc cgtcgacccc aaagtcagcg aagcgttttt
      481 tcgtgtcgat ccaggcacgg tccgcgctga accgtcgcct gagcttctcc gcgccgaagt
      541 gcagcctctg atcgaccagc tcggcgaagc ccccgatcct ttcgtcgtca tcgaaagttt
      601 gagcgagaca gctggcttca tgcctgccga gttccgggcg tttctcgcac atgaattcgc
      661 cctctccccc tatcccgttc tgcgtgacgc cgtgcccttg atgctgcttg acagcgaagc
      721 cgtggtccgg cgggctgccg ccgccgtcct cgagcaaacc gcggcagccg acacgctgtc
      781 gccggccgct ttgcgccgaa ccatcattct gcgcaattgg atcccggaag acgatcgcgc
      841 cggcgttgat cgcgccattc ggaaagcccg gaccaaaggg gtgatttgcg cgcaatggaa
      901 tgcgcctccc aagctctcaa tcctggcctt gccgattgac ggctccgggg ccaatggcct
      961 catcttgacg acaaaacccg gcaaaaccgg cttgtttggc ggcgttctat tgaagcagaa
     1021 ttttggcctc cgggacattt ggtgcgatcg cgacaagccg cgtcatgaga tcggccaagc
     1081 gatcgaggga ctcctggagg acaccgcggc aatcgaggtt gaaagacatt acctcgacgt
     1141 ggccttgcag catcatctcg cgatcgggct ttcgaacggc cgtctcccga cgccagcttt
     1201 gcttgccatc gcggaatccg ttggtggcgt cgagtggcgt gccagcagac tcgatatcga
     1261 tgcggaaatc gcgagggtct attccgaact ggatccggct aaaatagcgc cagcggacat
     1321 cgcggcgtcc ttgaaacgca ccggggagtg gttgcaccag gaaagcttcg cggagtcgtg
     1381 gtttgagaat gacgccgagg tttccgccat gattgacgaa atgtccgggc aggacgacga
     1441 cgccatcaca ggcgcggtcc tgagcgagct cctggagaaa cgccggggcg tttggacgga
     1501 acgttttttg ctgatggcgt tatgggcgcg tgcggcgaaa tccaaaaacg aaggacgcta
     1561 ttggcgcgac tttctgatcc ttgcacacga actgtccgcc ggccggccgc tagaggacgt
     1621 tccaattatg gcggcgatcg cgcagcgcac ggtgtcggcg gccctatcgg gaacgccgtt
     1681 ttgatttggc gaagcggatc tcgggtcgcc ccttgtcaag gctgaatgat gagtctgctg
     1741 gcttcgggca gtctcttgcc gagcgccatt cttactcctc aatcagctgc ccggtgcggg
     1801 ggcccgtcga gtctgtcgtc acgcttcttg aagcggacga cgacgccagg ggcacggcgg
     1861 cgcaagctga ttcggtttct gcccgttccg gtcaagacgt aatggccgcg gctgtcgtcc
     1921 cagcgcatca aatcggcttg gatggccttc tgacggagta cagcggtcat cggatcttga
     1981 gcgtgcgctc gcgcgacgtc gccatgggcg tcgaccttcg ttaaggcttc gatgagtcgt
     2041 tcttcctgag cgtcaatcat tgcgcttcgt acatgatagc gaggagtgat ctaggccagg
     2101 attttggcga gatcgcggcg cgagctcaac gagcccatcc aacgtcagcc cacagacttg
     2161 accggggcct cactgcacct ctgacgcgtt tgcattcgcc gttcaacatg acctgcccgg
     2221 tgatcgaagg catgcgcgag cgaggttttg gccggatcgt cagtatt
//
LOCUS       DATMGX010000205         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_134886, whole genome shotgun sequence.
ACCESSION   DATMGX010000205 DATMGX010000000
VERSION     DATMGX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134886"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..1288
                     /gene="scpB"
                     /locus_tag="VNR65_03335"
     CDS             470..1288
                     /gene="scpB"
                     /locus_tag="VNR65_03335"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF008506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HWJ17742.1"
                     /translation="MNQGNDGGATNSASSVASVETVGDIGVITLKNPAKRNALSHGMM
                     HALTEAFARFAEANIRVAILRAEPGVKVWSAGHDIGELPLGGMDPLGYADPMEETLRA
                     IRMFPGPVIAMVQGSVWGGAFDLVLSCDMVFADETATFAITPVNIGLPYNTTGLLHFM
                     GRLRLNVVKELFFTAAPITAEVALKWGILNHLVPSAELETYTMDRARQMAAKAPLAMA
                     VVKEQLRVLCDYQPIPAQVFERIQDMRRKVYESADYREGIAAFKEKRPPVFKGE"
     gene            complement(1396..1857)
                     /locus_tag="VNR65_03340"
     CDS             complement(1396..1857)
                     /locus_tag="VNR65_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HWJ17743.1"
                     /translation="MRKILIISAIAAGAAICTVAIGGETLSNSTRQDLLTAMKSEGLA
                     HLKYTLFAEQARKNGNEVLAGIFEHTADDEFEKHFKTHAKLYGLLKSDYENLLNAMSA
                     EFVESTQMYQEMAKRAEAAGDADAAKHFADFARDELEHRDIFKAAIPKQTK"
BASE COUNT          417 a          571 c          596 g          477 t
ORIGIN      
        1 gtgccggcgc gttaagcgcg gggcgtaggc attaatcctt cgttgtaggg acggtgatgg
       61 ttaacccctg gatggttaat tttgcgcgcc cggatttatt tccggtctaa attgcaccca
      121 acattggtta atgcgacgta aagcgagagc agcctgcgcc taggctgagc gcggataagc
      181 ctctggaaac gctcaagttt tcgagccaga aaaacagggt taatcgaatg tatttattcc
      241 cgaagtggga cgctctaaag tccgttaaat atattcgcgc cgcgcctgtt aaccatgccc
      301 gcgccctccg cagccgcgcc ggggcaagcg cgtcactcag atttatcgtg caatatcaag
      361 agctttccgc aattaactcc gcggaaaggc gcatttctgt aaaatcaacc gttattgaga
      421 tcaagcggtt tgagcctgac gaacggccaa acaatctgga ccagtcccga tgaaccaagg
      481 aaacgacggc ggcgctacca attccgccag ttcggtggcc tctgtcgaga ccgtcggcga
      541 tatcggcgtc atcaccttga aaaatccggc caagcgtaat gcgctcagcc acggcatgat
      601 gcatgcgctg accgaagcgt tcgcccgttt cgcggaagcg aatattcgcg tcgcgatctt
      661 gcgggcagag ccgggcgtca aggtatggtc cgctggccat gacatcggcg aactgccact
      721 cggcggtatg gatccgctgg gctacgccga tccgatggaa gaaacgctgc gggcaatcag
      781 gatgtttccg gggcctgtga tcgccatggt gcaggggtcg gtatggggcg gcgcatttga
      841 tctggtgctc agttgcgaca tggtgttcgc ggacgaaacc gcgacattcg ccatcacccc
      901 cgtgaatatc ggcctgccct ataatacaac gggccttctc catttcatgg gccggctcag
      961 gctcaacgtt gtgaaagagc tgttttttac cgctgctccc atcacggcgg aggtcgcgct
     1021 caaatggggc atcctcaacc atcttgtgcc ctccgctgag ttggagacct acacgatgga
     1081 tcgcgcacgg cagatggcag cgaaagctcc gcttgcgatg gctgtggtca aggagcagtt
     1141 gcgggtgctg tgcgactatc agcctatccc tgcgcaggtg ttcgagcgca tccaggatat
     1201 gcggcggaaa gtgtatgaaa gcgcggatta tcgggagggc atcgcggctt tcaaggagaa
     1261 gcgcccgccc gttttcaagg gcgaatgatt tgtgcaagtc gaggctggac tgtgaaggga
     1321 gcgagccttg ccgctccttt cggtgctatc gagcaaccgc ccttcgcggt gtcgcagtgc
     1381 cgaggatgcc ggcggctact tcgtctgttt cgggatcgcg gccttgaaga tatcgcgatg
     1441 ctcgagttcg tcgcgcgcga agtcggcaaa atgcttcgcg gcgtcggcgt caccggctgc
     1501 ctcggctcgc tttgccatct cctgatacat ctgggtggat tcaacaaatt ccgcggacat
     1561 cgcgttcaac aggttctcgt aatcgctttt caagagaccg tagagctttg cgtgcgtctt
     1621 gaagtgtttt tcgaactcgt cgtcggcggt gtgctcgaag atgcccgcaa gcacttcgtt
     1681 gccgtttttc cgagcctgct cagcaaacaa cgtatacttc aagtgggcta agccctcgct
     1741 cttcattgca gtcagcagat cctgccgcgt ggagttcgac agggtttcgc cgccgatggc
     1801 gactgtgcaa atcgcagccc ccgcagcgat cgccgaaatg attagaattt tacgcattgt
     1861 ttcctccctc ttttattttg ttcgaaagcc ccgagcggcg cagcccccat caggaatcgc
     1921 aatctcctgg caatgttgtt tagcttattt gatacattct gtcacaaccc agctgcggct
     1981 gcgccatgtc gtcgcgccgg gagaggccgt tatacgaacc catcctgtga gatttcgaac
     2041 ggttaaacac attggttcgt t
//
LOCUS       DATMGX010000206         5823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_250536, whole genome shotgun sequence.
ACCESSION   DATMGX010000206 DATMGX010000000
VERSION     DATMGX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250536"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VNR65_03345"
     CDS             complement(<1..523)
                     /locus_tag="VNR65_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWJ17744.1"
                     /translation="MFNPQFSQASRLRLRTTIRLRWFAVAGQTATILMLHYGFKFPVP
                     LALCLAVILLSSGLNTVLSISYPRSQLLSSQLALLLLSYDVLQLSALLFLTGGLENPF
                     AFLLIVPVAVSASTQRLVVTAMLAAFTVVLATILARYHWPLPWTDTEPPVLPLNYMLG
                     LWSALVSCIVFIAF"
     gene            691..1305
                     /locus_tag="VNR65_03350"
     CDS             691..1305
                     /locus_tag="VNR65_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWJ17745.1"
                     /translation="MKTGYAAFLALVLFAAGFAGAYALLNAPESGQSEGTPLIGGPFS
                     LVDTNGKRVTDRDFRGKLMLVFFGYTHCPDVCPTELQTMSTVMEKLGPEADKVAPIFI
                     SVDTKRDTPQALADYMKNFDTRITGLTGDQDDIASAAKAYRVYFRKAGDSGNGDYSVD
                     HSAFVYLMDGSGKYITHFTFNTAPESMAAVIKKKIAADNASNRA"
     gene            1453..2901
                     /gene="phaZ"
                     /locus_tag="VNR65_03355"
     CDS             1453..2901
                     /gene="phaZ"
                     /locus_tag="VNR65_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HWJ17746.1"
                     /translation="MLYHLYELSHAAMSPARAAANSTRLFLRNPFNPLSYTDVGKNAA
                     AAAELMERTTRRYKKPGFNIKATTVKGKRVEISEEIVWRKPFCDVIHFKKALPEGAPS
                     EPRLLIVAPLSGHYATLLRGTVEAMLPYTDVYITDWHDARLIPLREGTFSLDDYVDYV
                     IGIMEQFQGDVHVMGVCQPGVPVMSAVALMEARNHPFLPRSVILKGSPIDTRISPTAV
                     NKLAEEKGINWFRRTVITSVPWPNRGHGRLVYPGFLQLTGFMAMNLDRHMNAHKDLFV
                     NLVRGDGDSVDKHKEFYDEYLAVMDLAAEYYLQTIDQVFVKHQLPKGEMYHRGQLIDM
                     SAIRHVALMTVEGEKDDITGAGQTEAALHLCTNLSRSKKLHYTQPGVGHYGIFNGSRY
                     RQEVVPRVVAFMADHDVRGSALRRLWHRLAGDRDVAAPPEPLPPEEKVEDIILTEPLR
                     LSALPAKPVMRMKKGRLRTRARNGKIVRNRRR"
     gene            2995..3303
                     /locus_tag="VNR65_03360"
     CDS             2995..3303
                     /locus_tag="VNR65_03360"
                     /inference="COORDINATES: protein motif:HMM:NF021447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HWJ17747.1"
                     /translation="MTFKVGDIVQLKSGGPPMTVTRVNTNLVGFSEITCQWFYVQMET
                     GTFLPDVLIPSEPRPFEELKGASFPTVGPAMTGQLASQPASDNGLQEKPSPRSLLGTS
                     "
     gene            complement(3318..3989)
                     /locus_tag="VNR65_03365"
     CDS             complement(3318..3989)
                     /locus_tag="VNR65_03365"
                     /inference="COORDINATES: protein motif:HMM:NF018053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HWJ17748.1"
                     /translation="MRERFQRFAIYWTPAPETAMAEFGALWFGGSETFGLPPELVARA
                     TKAPAVYGLHATFKAPFPLNDDVAPEALRDALDDFCANRRAPATGRLILSHHQHYLTL
                     MLSGDEADTDWLAAQCVTRFYRFRAPPNEGENKRWKLDGLSPQEEAFLKEFGYPYVLS
                     AFRFHISLAGPLDSAELDAVAKALEPHLAPFMRETFQIKDLSLLGEPRSAGPFEVISR
                     HRLGR"
     gene            complement(4191..4916)
                     /locus_tag="VNR65_03370"
     CDS             complement(4191..4916)
                     /locus_tag="VNR65_03370"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWJ17749.1"
                     /translation="MSPSQAERLRAMIEEDILSFKLKPGDKLDECRLAEMYGTSRTPV
                     REALRQLSSNGLIEIRPHKGAIVAKLGIRELVELLEVMAELEGACGRLAAKSCMNSDV
                     DAIIAAQETCRKYADLRDAHGYQKANEAFHEAIYHASRNECLVKLTLSIRNRVAAYRR
                     LRLEQVSRLKYSVAEHDRILHAIQESLPEEADRLLQLHILNLGSELRRMFSVISTEDW
                     PAPSEPREARVADMAAMESVSVK"
BASE COUNT         1301 a         1620 c         1686 g         1216 t
ORIGIN      
        1 agaaggcaat gaacacgatg cacgatacaa gggcggacca aagcccaagc atatagttca
       61 gcggcaaaac cggaggctcg gtgtccgtcc agggcagggg ccaatgatag cgtgcaagga
      121 tagtggccag gaccacggta aacgccgcca gcatcgcagt gacgacgagc cgctgcgtcg
      181 acgcggacac cgcgaccggg acaatcagca ggaaggcaaa tggattttcg aggccgcccg
      241 tcaggaaaag gagtgccgat aattggagca cgtcatagct gagaagcagg agtgccaatt
      301 ggctggagag gagttgcgag cgcggatagg agatgctcag caccgtgttc agccccgaag
      361 acaggagaat gacggccaga cagagcgcaa gcggtacggg aaacttgaat ccataatgga
      421 gcatcaaaat ggtcgcggtt tgccccgcga cggcaaacca tctgagccgg atcgtcgttc
      481 ggagccgcag ccggctcgcc tgggaaaatt gtggattgaa catgagacgg tcctgaaatg
      541 tgcgaacgtc gggaggcgtg catgctttgg taaatggaac gtgcctgatg gtccggctcg
      601 ccgtgcctca ttgacgggac tttcactaga gaccacgtct tcctgcaaag cgcacgcaaa
      661 atcaatggca tttcgacaag ggcgacaggc atgaaaaccg gctatgcagc atttctcgcg
      721 ttggtgcttt tcgcggcggg ttttgcgggt gcatacgcgc tcctgaatgc ccccgagtca
      781 ggtcaaagcg aaggcacgcc cctcattggc ggtccatttt cactcgtcga tacaaatggg
      841 aagcgcgtca ccgaccgcga tttccgcggc aagctgatgc ttgtcttttt cggatatacc
      901 cactgccccg atgtctgccc gacggagctg caaacaatgt ccaccgtcat ggagaagctc
      961 gggcccgaag ctgacaaagt cgcgccgatc ttcatttccg tcgatacgaa gcgcgatacg
     1021 ccgcaggcgc tcgccgatta tatgaaaaac ttcgacactc gtattacggg attgaccgga
     1081 gaccaagacg atatcgcaag cgctgcaaag gcttatcgcg tctacttccg gaaagcggga
     1141 gacagcggga acggcgatta ctccgttgat cactcggctt tcgtgtatct catggacggt
     1201 tcaggcaaat atatcactca cttcacgttc aatacggcgc cagaatccat ggcggctgtg
     1261 ataaaaaaga aaattgcggc tgacaacgcg tccaatcgcg cctaagctca accaaagatt
     1321 aaattgcaat actcattttt gcatgtcagc cttgaaggcc gatcacggtc aagtttcggt
     1381 tacattgctc tttacagcgt tttcttgggt tgctttgagt aggtcgggcg atcactctga
     1441 gaaggagatc acgtgctcta tcatctttat gagttgagcc atgccgcgat gtcccccgcg
     1501 agggctgcgg caaattcgac ccggcttttt ctgcgcaatc ctttcaatcc actttcttac
     1561 acggatgttg gaaagaatgc cgcggcggcc gccgagttga tggagcgcac cacccgccgc
     1621 tacaaaaagc ccgggttcaa catcaaggcc acgaccgtca agggcaagcg ggtcgagatc
     1681 tccgaagaga tcgtctggcg caagccgttt tgcgatgtga tccatttcaa gaaggcctta
     1741 ccggaaggcg cgccgagcga gcccaggctt ctgatcgtcg cccctttgtc gggccattac
     1801 gcgacgcttc tgcgcggaac cgtggaagcc atgctgccct ataccgacgt ttacatcacc
     1861 gattggcacg acgcccgcct gatcccgctt cgtgagggca ccttctctct cgacgattac
     1921 gtcgactatg tgatcggcat catggagcag ttccagggcg acgtgcacgt catgggggtg
     1981 tgtcagcctg gcgtgccggt catgtcggcg gtcgccctca tggaggcgcg caaccatcct
     2041 ttccttccaa gaagcgtgat cttgaaaggc agcccgatcg acacgcgcat cagtccaacc
     2101 gcggtcaaca aacttgcgga ggaaaaaggc atcaactggt tccgccggac ggtcatcacc
     2161 tccgttccct ggccgaaccg cggacatggg cgtcttgtct atccaggctt ccttcagctt
     2221 acaggcttca tggcgatgaa cctcgatcgc cacatgaatg cgcacaagga tctgttcgtg
     2281 aatctggtgc gcggcgacgg tgactctgtc gacaagcaca aagagttcta cgacgaatat
     2341 ctcgcggtca tggaccttgc ggcagaatat tatctgcaaa caatcgacca ggtgttcgtg
     2401 aagcatcagc ttccaaaggg cgagatgtac catcgtgggc aactcattga tatgagcgcc
     2461 atacgtcacg ttgccctgat gacggtcgag ggcgagaagg acgacattac gggcgctggc
     2521 cagacggagg ccgccctcca tctttgcaca aatctgtcac gctcaaagaa gttgcactac
     2581 acgcagcccg gcgttggcca ttacggcatt ttcaacggct cgcgctatag gcaggaggtc
     2641 gtgcctcggg tggtcgcttt catggcggac catgatgttc ggggcagcgc gctccgcagg
     2701 ctgtggcacc ggcttgccgg ggaccgggat gtggcagctc cgccagagcc attgccgccc
     2761 gaggagaagg tggaagatat catactgacc gaaccgctgc gtttgtcggc tctgccggcg
     2821 aaaccggtga tgaggatgaa aaaagggcgc ttacggactc gcgcacgtaa cggcaaaatc
     2881 gtgcggaatc gccgccggta ggtttggcga aaccctgcgc gcagcctctt ccgaaggctg
     2941 cgcggtcttg gatttctaca cgggttcagg atttgcacgg cctcaggaat tgcaatgacc
     3001 ttcaaggttg gcgatatcgt tcagctcaag tcgggcggcc cgccgatgac cgttacgcgg
     3061 gtgaacacca atttggtggg tttctccgag atcacgtgcc aatggttcta tgtgcagatg
     3121 gaaacgggca cgttcctacc agacgtgtta atcccaagcg aaccaagacc attcgaagag
     3181 ctgaaggggg cgtcgttccc aacggttggg ccagcgatga ctgggcaatt ggcgagccag
     3241 cctgcatctg ataacggtct gcaagaaaag ccatcgccgc ggagcttgct gggcacttcg
     3301 tgagactgag caatctttca tcggccaagt ctgtgcctgc ttatcacttc gaaaggccct
     3361 gcgcttcgag gctcacctag aagcgacaaa tccttgatct gaaacgtctc ccgcatgaag
     3421 ggagcaagat ggggctcaag cgccttggca accgcgtcca gttccgcgct atcaagcggc
     3481 cctgcaagcg aaatgtgaaa gcgaaaagcg ctgagcacat agggatagcc gaactccttc
     3541 aggaatgcct cttcctgcgg cgacaggcca tctaacttcc aacgtttatt ctccccctca
     3601 tttggcgggg cgcggaaacg atagaaccgc gtgacgcatt gggccgcgag ccaatctgtg
     3661 tccgcttcat ccccgctcag catcaaggtg aggtagtgct ggtgatggct caagattagc
     3721 ctgccagtgg caggcgcgcg cctgttggcg cagaaatcgt cgagtgcgtc gcggagcgcc
     3781 tccggtgcga catcgtcatt cagcggaaat ggcgctttga acgtggcatg gagtccatag
     3841 acggcggggg cttttgttgc ccgtgcgacg agttccggcg ggaggccaaa tgtctcagag
     3901 ccgccgaacc agagagctcc aaactccgcc atcgcggttt cgggagcggg tgtccaatag
     3961 atggcgaagc gttgaaagcg ttctcgcatg aaaaacccgc tgtcaggcta accgcgcgtc
     4021 cgaagcggag cgctgttcaa cctgagccgg atggatgtca ttcacgtggc atcacgtatg
     4081 cgttcatgac gtgatttgcc ggcatcctgt gctctgcgta ctacccattg cctgctggtg
     4141 cgcccgctat attttacggg cactcgcagg agcgatcaaa gctcgcactg ttatttgaca
     4201 gatacgcttt ccatagccgc catgtctgca acgcgcgcct cacgcggctc cgacggcgcg
     4261 ggccaatctt ctgtggatat gacggagaac atgcgtcgaa gctcactgcc gagattcaaa
     4321 atgtgcaatt gcagcagacg atcggcttct tcgggaaggc tttcctggat cgcgtgcaat
     4381 atccggtcat gctcggcgac ggaatattta agccggctca cctgctccag tcgcaatcgc
     4441 cgataggcgg ccacgcgatt acggatgctc agcgttagct tgacgagaca ttcattgcgg
     4501 cttgcatggt aaatagcctc gtgaaaagcc tcgttggcct tctgatagcc atgcgcatcg
     4561 cgcaggtcgg catatttgcg acaggtttcc tgagccgcga tgatcgcatc cacgtcgctg
     4621 ttcatgcacg attttgcggc taggcgacca cacgcgcctt cgagttcggc catcacttcg
     4681 agcaactcga cgagttcccg aatgccaagc ttcgcgacga tggcgccttt gtggggcctg
     4741 atctcaatta gcccgtttga agagagctgc cggagagctt cgcgaaccgg ggtgcgagac
     4801 gtgccataca tctcggcaag tctgcactca tcaagcttgt ctccaggctt caacttgaat
     4861 gatagaatgt cctcctcaat catcgctcgc aagcgttcag cttgtgacgg actcattctc
     4921 cctcgctcct aaaatccaac actgcatagg cctttcatct ccagacaaaa ggctaccaac
     4981 ttccgaaaga aaagtgaagt acaggcgctc gcccggacaa cgttttcttt aaattgctcg
     5041 cttgaagcag gaagaaggat atcatctgct tttgcctgcg tttcgacttc cctcaacggc
     5101 aagtcgagtc tgtcatggat ggtctcatat ttttcgggaa aacttgtcct caacgaggcc
     5161 tgcaccctga aaactttgta atttgcatcc aaatccagtg cgacaagggg ccgccacttg
     5221 tagcagcggc acaaagctag attgacgctt gaaagcgccg cagagtactg gaatcatatc
     5281 tacaactcgg accataccga agcggaacag acactgattt gccttgcttg gcaagcagtt
     5341 tatccagatc ggaaaagcag taccccatcc tggatgacac cgatggagcg aaacgggccg
     5401 tttgcggcct tccgcgcacc atttccgaag cctctccacg cctctctggg ttgccggtct
     5461 gagtgacaat cgagatcatg aactgcggga catcgagcat aaccgcgagt attatgtgag
     5521 cggggcatgg tgtcttagag gacaccggtt cataacccag ctcgcgctcg tagcctcgag
     5581 cgcatttccg gtgaaactca aggtgggcgt cgtgcggtag cgcgccgcta agaagtgagg
     5641 cattcccatg agatggcaag gtcgccgtga gagcgagaac gtggaagacc gccgcgacga
     5701 aggcggtgcg gatgacgaag gtggcggcgg gggcttcggt tttccttttc caggcggagg
     5761 aggcggcgga ttccagatcc cgatgggcca gggtggcggc ggcattggta tcatcggcct
     5821 gat
//
LOCUS       DATMGX010000207         2500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_201660, whole genome shotgun sequence.
ACCESSION   DATMGX010000207 DATMGX010000000
VERSION     DATMGX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201660"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VNR65_03375"
     CDS             complement(<1..584)
                     /locus_tag="VNR65_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWJ17750.1"
                     /translation="MTTQGLTTKDAIEQRRSIRKFKNDRIPEEHILALIEAARLAPSA
                     SNTQPWRFKIVSDDETKAELAEAAYHQSFVGQAPVVLVCCADLQGYFEGIAARSQDFG
                     KEGALEESMVRVLRERAEQLKTMNVQDMAPGIAFSVAIAIEHIVLRALDFGLGSCWVR
                     MIDEQKIKGIFGWSNAMHVVALLPLGYPDEAPAPR"
     gene            complement(630..1070)
                     /locus_tag="VNR65_03380"
     CDS             complement(630..1070)
                     /locus_tag="VNR65_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWJ17751.1"
                     /translation="MDFNECIRFATEHPICAAATVDGDQPRVRMLGMWFAAKEGFYFS
                     TAKVKAMCRQLRANDKVELCFYAPPGRPPGEGGTMDIGKVMRASGVVAFLDDPKLKER
                     LLQERPFLRPTAENIAIFRVEKGDAWFWTFSDSGRESAIERISF"
     gene            complement(1344..1694)
                     /locus_tag="VNR65_03385"
     CDS             complement(1344..1694)
                     /locus_tag="VNR65_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17752.1"
                     /translation="MQRMMTSGAAKEGSNVGENVAETNQKERRDQFFCQLAHRHLYAG
                     ESDKDGCVVVLEATGTSAGHNEREIQGFLERLVEDGWIEVKDPVRAGKIRARLTPAGR
                     ARAQVICKDVPFAR"
     gene            complement(1902..>2500)
                     /locus_tag="VNR65_03390"
     CDS             complement(1902..>2500)
                     /locus_tag="VNR65_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ17753.1"
                     /translation="SPAERSRDEWQKEQALFAKISDQIFYMDKIVGDLQDYARPLVPE
                     REEIVVEKLIEDVVQSLPHTDGVRIATEVPDLQLLADPHLIHRVLANLILNAVQAMPD
                     GGTVTVSASTDDGSVAISVRDTGIGIPNDAKDKLFKPLFTGKAKGTGLGLAVVKRIVE
                     AHAGQITVESEVGKGSTFTVNLPSAEELKVERDSSFRS"
BASE COUNT          526 a          766 c          620 g          588 t
ORIGIN      
        1 cgtggagcgg gagcctcatc agggtagccg agcggcagca gcgcaacgac gtgcattgcg
       61 ttactccagc caaaaatgcc cttgatcttt tgctcatcga tcattctcac ccagcaactc
      121 cccaagccaa aatccaaggc ccgcaacaca atatgttcaa tcgcgatagc gacgctgaac
      181 gcaataccag gagccatatc ttggacgttc atcgttttca gttgttcggc ccgctcacga
      241 agaacgcgca ccatgctctc ctcaagagcg ccttccttcc caaaatcttg gcttctggca
      301 gcgatgcctt cgaagtatcc ctggaggtcg gcgcagcata ctagcaccac cggagcctgc
      361 cccacaaatg actggtgata cgctgcttct gccagttcag ccttggtctc gtcatcggaa
      421 acaatcttga accgccacgg ttgcgtattg gaggctgagg gcgccaatct agcagcttct
      481 ataagcgcca aaatatgctc ttctggaatg cgatcgtttt taaatttccg aatgctgcgc
      541 ctttgctcga tcgcgtcttt ggtggtaagt ccctgagttg tcatatcgct ctccgttcga
      601 tctctggttt cgcttcttcc tgtgctcact taaaaggaga tccgttcaat tgcagactcg
      661 cgcccgctgt cggaaaaggt ccagaaccac gcatcgcctt tctcaacgcg gaatatggcg
      721 atgttctcag cagtaggacg caagaacggc cgctcctgaa gaaggcgctc tttgagcttc
      781 gggtcatcaa ggaacgcgac cacgcctgac gcccgcatca ccttccctat gtccattgtt
      841 cctccctcgc caggcgggcg tcccggtggg gcgtagaaac acagctcaac cttgtcgttt
      901 gctctcagct gccgacacat cgcttttacc ttcgcggtgg agaaatagaa gccttccttg
      961 gccgcaaacc acattcccaa catgcgaacc cgcggctggt cgccgtcgac ggtcgctgcc
     1021 gcacaaatgg ggtgctccgt tgcaaaccgg atgcattcgt tgaagtccat cgtaccatca
     1081 ccgctcgttc gtgtgcattc attacttaat tagctggtgg gaagtatatg gactctcgcc
     1141 gtcgtcatgc cgtcagatgt gacacgcgcg tgagcacgtc aataaaaccg cccggcgaaa
     1201 actccagagt gtcagcggac aagcgacgcc cgatgttaga tgtggctagc cttcgaccgc
     1261 tacccgcaac agctatttct cggcctctct ccaccactag gtcccccgcc attcttccac
     1321 gcacgaagat tgcaggccaa ctgctatcga gcaaacggca cgtccttgca gatcacttgc
     1381 gcacgcgctc tgcctgctgg cgtcaaccgt gcacggattt tgccggcccg aacaggatct
     1441 ttcacttcga tccacccgtc ctccacgagg cgctcaagaa agccttgtat ttcacgttca
     1501 ttgtgacctg ctgacgttcc cgttgcttcg agaacgacca cgcagccgtc tttgtcgctt
     1561 tcaccggcgt aaagatggcg atgtgcaagc tggcagaaga actgatctct gcgttctttt
     1621 tgattggtct ctgcgacgtt ctcgcccacg tttgacccct ctttggcggc gccggatgtc
     1681 atcatccgct gcatttgtgg ccctaactcc cacaagacgc accgtcttca gcgcccgcga
     1741 cgaactccaa acggagtgcc gctaacaact ggacgacgca tcccctttct tcaccatggg
     1801 tttccgcggt atgaataaga accttccacc aagaaagttc gctttaggaa ccaaaaacaa
     1861 agaaaaaggg gggcgtggta gcttcaacag ctccagacac gctaacttct gaacgacgag
     1921 tcacgttcga ctttcaattc ctcagcgctg ggcaaattta cggtaaacgt cgatcctttt
     1981 ccgacttcac tctcaacagt tatttgtcca gcgtgcgcct cgacgatccg cttcaccact
     2041 gccaacccaa gtcccgttcc cttcgccttg ccggtaaaga gcggtttaaa gagcttgtct
     2101 ttagcgtcgt tcggaatgcc gatgccggta tcgcgcacgc ttatcgccac cgagccatcg
     2161 tcggtagatg cactaacggt gaccgtcccg ccgtccggca ttgcctgcac ggcgttgagg
     2221 atgaggtttg ctaacacgcg atgtataagg tgggggtccg ccaacaattg caggtctgga
     2281 acctctgttg caatgcgcac accatccgtg tgtggcagcg attgcacgac gtcttcgatc
     2341 agcttctcga caacgatctc ttcgcgctca gggacgagtg gacgggcgta gtcttggagg
     2401 tcgccaacaa tcttgtccat gtagaaaatc tggtcgctga tcttggcaaa gagcgcttgt
     2461 tctttttgcc actcgtcgcg gctgcgctcg gcgggagaca
//
LOCUS       DATMGX010000208         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_78854, whole genome shotgun sequence.
ACCESSION   DATMGX010000208 DATMGX010000000
VERSION     DATMGX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78854"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VNR65_03395"
     CDS             <1..226
                     /locus_tag="VNR65_03395"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HWJ17754.1"
                     /translation="SLTVRITRGENAGSAVTYTNVVRELTEAGDWLGAPTSFALPMSS
                     LSHDGDMLVVFLQTEELGPIVAAARVAGGW"
     gene            244..663
                     /locus_tag="VNR65_03400"
     CDS             244..663
                     /locus_tag="VNR65_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWJ17755.1"
                     /translation="MAFWLFKTEPGTFSWDDQVGAGPKGEEWNGVRNALAQKNMRTMK
                     VGDLGFFYHSVHEKQIVGVVKVIHEIHPDSTDATGRWNCVDVAAVRPFPRPVTLSAIK
                     DEPRLANMALVKNSRLSVQPVTDEEWALVCQMGGYKS"
     gene            complement(720..977)
                     /locus_tag="VNR65_03405"
     CDS             complement(720..977)
                     /locus_tag="VNR65_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17756.1"
                     /translation="METEYDRTAEEHGKGIHEASIGVEEAPMPTETEAGPENGHDAPM
                     LPGPDLILDERLTSAAAPFLYMLSVVLIAVAGYFVYLFFHW"
     gene            1105..2049
                     /locus_tag="VNR65_03410"
     CDS             1105..2049
                     /locus_tag="VNR65_03410"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ17757.1"
                     /translation="MALTIALTINPLFAAEKLRFGLNWLPEAEHCGFFQAKAAGLYDK
                     AGLDVELKPGGPDVNTPLLVGVGQLDLAIGTGFTTLNMVEEGIPGVTVAAFFQKDPQT
                     LVAHPDQGIVTLADVKGRPVMISSEARQEFWQFLKLKFGFKDNQLRPYTYSPAPFLAD
                     PKAIQQGYITEDALLRGSQMPKPPVILLLADYGFENYTTTAFGLRSYIEKHRDAVQAF
                     INATRKGYDACMAGDYAAGMKLLLSMNPDHGEPLFHFKIKEMKERGLVDGGDAAARGV
                     GAMTDERWKSFFDTMAAAGIYSPKLEYKAAYTLDFVKK"
     gene            2049..2288
                     /locus_tag="VNR65_03415"
     CDS             2049..2288
                     /locus_tag="VNR65_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ17758.1"
                     /translation="MLDRPSSQTVLPPLLAMQGVRKTFANGLLVLDGVTLTLPGEPQF
                     LAVLGPSGCGKSTMLRIAANLDTPSAGRIDWPAFD"
BASE COUNT          489 a          723 c          766 g          518 t
ORIGIN      
        1 ttccttgaca gtgcggatca cgcgcggcga gaatgcgggg agcgccgtga cttatacgaa
       61 tgtggtgcgc gagcttacgg aggcggggga ttggcttggt gcgcccacgt cctttgcttt
      121 gccaatgagc tcgctctctc atgatgggga tatgcttgtg gtctttctgc agacggagga
      181 actcggtccc atcgtagccg cggcgcgcgt ggctggggga tggtagcagg gggctgctcg
      241 tgcatggcct tttggttgtt caaaacggaa ccgggaacct tttcctggga cgatcaggta
      301 ggggctggac caaagggcga ggaatggaac ggcgtgcgca atgccctggc tcagaaaaac
      361 atgcgcacga tgaaagtcgg ggacctcggc ttcttctatc actcggttca cgaaaagcag
      421 atcgtcgggg tcgtgaaggt catccatgaa atccatcccg actcgacgga tgctacgggc
      481 cggtggaatt gcgtcgatgt cgctgcggtg agaccttttc cgcggccggt tacgctctcg
      541 gcgatcaagg acgagccaag acttgccaac atggcgctcg tgaagaattc ccggctatcc
      601 gtgcagcccg tcacggacga ggagtgggcg ctcgtctgtc agatgggcgg ctacaagtca
      661 tagtttcggc ccgcttgcct tcaccgctca gaactcagcg ctatgggcga taatgcttgc
      721 taccaatgaa agaacaggta gacaaagtag cccgcgaccg cgatcaagac gaccgaaagc
      781 atatagagaa aaggagctgc ggcggaggtg aggcgctcat cgaggatcaa atccggccca
      841 ggcaacatag gagcgtcatg gccattttcc ggccctgcct ccgtctccgt tggcatcggc
      901 gcttcttcga cgccgattga agcctcgtgg attcccttgc cgtgttcttc ggccgtcctg
      961 tcgtattccg tttccatcga acagccccac gtttacggac agcacttaac ttctctgcgg
     1021 acgctgctcc ggcctgccct ggagggagcg cgctcactgt gtaagggtgc agtgcacgct
     1081 ttttggcggc cctttgcctt aagcatggcg ctgacgatcg cactcacaat aaatccttta
     1141 ttcgccgcgg aaaagctccg gtttgggctc aattggcttc ccgaggccga gcattgtgga
     1201 ttttttcagg cgaaagccgc cgggctttat gacaaggccg ggctggatgt ggagctaaag
     1261 ccgggcggac ccgacgtgaa tacgcccctt ctcgttggcg tagggcaact cgatctcgcc
     1321 atcgggacgg gttttacgac gctgaacatg gtggaggaag ggattccggg cgtgacagtc
     1381 gcggcgttct ttcagaaaga cccgcaaacg cttgtcgcgc atcccgatca gggcattgtg
     1441 accttggcag atgtgaaggg ccgcccggtc atgatctctt ccgaagcccg gcaggagttc
     1501 tggcagttcc tcaagctgaa attcggcttc aaggacaacc agcttcggcc ctacacctat
     1561 tcgcccgcac ctttcctggc agacccgaag gcgatccagc agggctacat cacagaggac
     1621 gcgctcctgc gcggctcgca aatgccgaag ccgcccgtga tccttcttct ggccgattac
     1681 ggctttgaga attacacgac gacagcgttc ggccttcgaa gctatatcga aaagcaccgg
     1741 gatgcggtgc aggctttcat caacgccacc cgcaaaggct atgatgcgtg catggccggt
     1801 gattatgcag cgggcatgaa gcttctgctc agcatgaacc ccgatcacgg ggagccgctt
     1861 ttccacttca agatcaagga aatgaaagag cgcggcctcg tcgacggcgg agacgcggca
     1921 gcgcggggcg tcggcgcaat gacagacgag cgctggaaat cctttttcga caccatggcc
     1981 gcagccggca tctactcgcc gaagctcgaa tacaaggcgg cttatacgct cgactttgtg
     2041 aagaaataat gctggaccgg ccctcctcgc aaacggtcct gccgccgctt cttgcgatgc
     2101 aaggggtaag gaagacgttt gctaatgggc ttctggtcct tgacggcgtt actcttaccc
     2161 ttccgggcga acctcagttc ctcgctgtcc ttggaccaag cggctgcgga aaaagcacca
     2221 tgctgcgcat cgcggcgaac ttggacacgc cgagcgcggg ccgcatcgac tggcccgcct
     2281 ttgattgagc gggcgagcat agcggcggtt tgaacctggg atacgtcttc caggagccga
     2341 ccctgatgcc ctggtcgacc acattcgaca atgtccgcct gccgcttcgc atccgggggc
     2401 acagcggcag cgcggtctcc gatcgcgtca tggacatgct ggtccgcgtc ggccttcggg
     2461 atttcgcgcg cgcttatccg cgcgagcttt ccggag
//
LOCUS       DATMGX010000209         2920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_457520, whole genome shotgun sequence.
ACCESSION   DATMGX010000209 DATMGX010000000
VERSION     DATMGX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457520"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VNR65_03420"
     CDS             complement(<1..254)
                     /locus_tag="VNR65_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17759.1"
                     /translation="MGTRRPATTSAWCHEDPHFAPTGPARYDMQMNAHQTKQVTRGPS
                     GITSSGWRELLRQTWRAASSQHASLNAAGIAFFGVWALFPA"
     gene            678..1631
                     /locus_tag="VNR65_03425"
     CDS             678..1631
                     /locus_tag="VNR65_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17760.1"
                     /translation="MLVLDRPIRRWSKADQIGVEQDARYYEYSKAANPIGAKLISRVP
                     YHSFLPDLYDSGPTRSVTLDLSHELNTEWPATGPGLCAHFLRILREESLELKPNATSM
                     VLYVIKGSGEVSQGGTRFGFAAGGFITLPGGTSAKLMASVETTMYYVDDEPLMQYLGA
                     SVAAPRFKPTYYSPELAQAELRKVAADPSAHKRSRVSVLLGNANFPQTRTATHTLWAM
                     YGLLPAHSEQKPHRHQSIALDFIVEAPAGCYSLVGTKLDADGNIASPQRVDWISGMAF
                     VTPPGYWHAHYNESDKEALLIPIQDAGLQTYLRALDIRFEH"
     gene            1692..2672
                     /locus_tag="VNR65_03430"
     CDS             1692..2672
                     /locus_tag="VNR65_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ17761.1"
                     /translation="MDYLAAMRAFVRSADLGSFSKAAAESGAKISTVSRHISALEADL
                     GAALLNRSTRRIALTEAGRAFYERAVLIVAEVEDARRMTSALNSRPRGLLRLNLPGAF
                     GRLHVMPAVPGFLTAHPDIRIEVGFSDARVDLIETSTDLVIRIGSLTDSGLTAKRLAS
                     HHRLLCAAPQVLSRLPNLEQPTGLSAVECLSMAPKERWHFRPSTGPAAAPAQDLVSVS
                     GKLCTSDLQALRVSVLSGLGVALLPAWLVGADIAAGRLVRVLSGWDFSVTDDFDQAIW
                     ALYPPKKIVSPKVRAFMDYLQGHLADTLSTPQPGSIADRRLRAPTKKNPR"
BASE COUNT          572 a          896 c          842 g          610 t
ORIGIN      
        1 gcggggaaga gcgcccatac accaaagaac gctatcccgg cagcattcag cgacgcatgc
       61 tgcgacgatg cagcacgcca tgtttgcctc agaagttctc gccagccaga actcgtgata
      121 ccgcttgggc ctctcgttac ctgcttcgtc tgatgagcgt tcatttgcat atcgtagcgc
      181 gcggggccgg tgggggcgaa atgcgggtcc tcgtggcacc atgccgaggt ggttgccggc
      241 cggcgcgtgc ccaatgcgtt gcgcgctccg tttccgaagt ggggcggtgc agccaccagt
      301 cgagctagat gaagcgcaag ccgtcggatg tcgcagccca gggcgcgatt gtgcagttcg
      361 cgaatcgcgg ctgtcgggcg cgcaatatga gtagctctac gtctgcggtt agccgagggt
      421 gaccacgaat gggcatattg acccgcttcg gccggtctcc attggctgct agcgtgcatc
      481 aaaacgaatc cgacaagcca gccattaatc aagcaactcg aattgcaact gcaatccaat
      541 aggcccgtta caagctgggc aaacgtgccc cattctgcaa aagtcatgtg ccagggcagg
      601 gctgtcgggg aggagcgaat gtccctacat tatctccatg ccagcgcttt cgctgacggt
      661 aaccgaagga gttcatcatg ttggtgcttg accgccccat ccgccgttgg tccaaagccg
      721 atcaaatcgg tgtggaacag gacgcgcgct actacgagta cagcaaggca gccaatccca
      781 tcggggccaa gctgatttcg cgagttccct atcacagctt tctgcccgac ctttacgatt
      841 caggccccac gcgttcggtg accctggatt taagccacga actcaatacc gaatggcctg
      901 ctacggggcc tggattgtgt gcgcacttcc ttcgaatact gcgcgaagaa tcgctcgaac
      961 tcaaaccaaa cgccacttcg atggtcctat acgtgatcaa agggtcaggc gaggtgtcgc
     1021 aaggcgggac ccgatttggc ttcgctgccg gtggttttat cactttgcct gggggcacat
     1081 cggcgaaact tatggcgagc gtcgagacga cgatgtacta cgtcgatgat gagccgctga
     1141 tgcagtatct gggtgcgtca gtcgccgccc cccgcttcaa gccgacgtat tattcgccgg
     1201 aacttgccca ggcagagctc cgtaaagtgg cagccgatcc gagcgcccac aagcgcagcc
     1261 gagtcagcgt gcttctcggc aatgcgaatt tcccccaaac gcgaaccgcg actcatacgc
     1321 tttgggcgat gtatggcctc ttgcccgcgc attccgaaca gaagccgcat cgtcaccaat
     1381 ccatcgctct cgacttcatc gtcgaggcgc cggccggctg ttactcgctc gtgggaacca
     1441 agctggacgc ggacggtaac atcgctagtc cgcagcgcgt ggactggatt tcggggatgg
     1501 cgttcgtcac gcctcccggt tattggcatg ctcactacaa cgagtcggac aaagaggctc
     1561 tcttaatccc gatccaggac gccgggctac agacttatct tcgcgccctg gacatacgat
     1621 tcgagcactg aggagaagcg aaagcgcatt cgacttgccc gtgccggtcg gatgcgctct
     1681 aaactgagcc catggactat ctcgctgcga tgcgcgcttt tgttcgctca gccgacctgg
     1741 gaagcttttc caaggcggcg gctgaaagcg gcgccaaaat ctcgaccgtt tcgcgccata
     1801 ttagcgcgct ggaggccgac ctaggcgctg cactgctgaa ccgctcgact cgccggatcg
     1861 ctcttacgga ggccggccgg gcgttctatg agcgcgcggt cttgatcgtt gccgaagttg
     1921 aagatgccag acgtatgact tccgcgctga acagccgtcc tcgcgggctg cttaggctaa
     1981 atcttccagg ggcgttcgga cgcctgcatg tcatgccggc tgtgcccggc tttcttacgg
     2041 ctcatcccga catccggatc gaagtaggat tctccgatgc acgcgtggat ctcatcgaaa
     2101 ccagcacgga tcttgtcatc cgcatcggct cgctgaccga ctcggggtta acagcgaagc
     2161 ggcttgcgtc gcaccatcgt ctgttgtgcg ctgcgccgca ggtgctatcc agactcccca
     2221 atctggaaca gcccacaggc ctgtcggctg tcgaatgtct atcaatggct ccgaaagagc
     2281 gctggcattt tcggccatct accggtccgg cggcagcgcc tgcccaagac ctcgtctcag
     2341 tctcgggcaa actttgtaca tccgaccttc aagcgcttcg agtgtcggtg ctcagcggcc
     2401 tcggcgtcgc cttacttccc gcctggttgg tcggcgctga catcgccgcc ggtagattgg
     2461 ttcgagtact gtctggctgg gatttctcgg taacggacga tttcgatcaa gcgatctggg
     2521 cgctttaccc acccaaaaaa atcgtctcgc cgaaggtgcg cgccttcatg gactacctac
     2581 agggacacct cgccgacaca ctatcgacac ctcaacccgg ctccatcgcg gaccgacgcc
     2641 tgcgtgcccc gaccaagaaa aatccgagat gaaagattca ctccttggcg aactgctggc
     2701 gattcggcat atgtgtgagc gaccgctttt gggtgcgaac caagagcgcg ggcggtagtc
     2761 cgaatctggc caatctcaga ccgatgcttc gcttcgaacc ggccgttccg cggaatctaa
     2821 cgggtaaatg tagctggtgg ccggcaacgt gccaagtcga cctcttcctg ccgttgatgc
     2881 cggcagcttt gcggctttcg acctgcaatc gcgctgactg
//
LOCUS       DATMGX010000210         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_173854, whole genome shotgun sequence.
ACCESSION   DATMGX010000210 DATMGX010000000
VERSION     DATMGX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173854"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1182)
                     /locus_tag="VNR65_03435"
     CDS             complement(202..1182)
                     /locus_tag="VNR65_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HWJ17762.1"
                     /translation="MANRRTVAAIIASCLVWSGSAAADEKPIAEQIVDAMTKVFGVHK
                     GFRANHAKGIVVEGSFKATPEAATVSKAVIFDGSEIPLTARFSDATGIPNIPDGIPDA
                     NPHGIAIKFHLPDGSDSDLVINALKFFPVSNGADFRDLFLAIAASPPDAPKPTKIEQF
                     VAAHPSIAAANAAVHTPDSFAHEEYRGVNAFVFVNKTGQKQAFRYHLVPERVVHLDPA
                     EAQKKAPYFLIDEVQERLKKGPITFHLKAQLAAPGDQTNDGSKAWPDDRKVVDMGVLT
                     ISKVSADPFEAQNKLLFLPGQLLDGIEQSDDPMIDIRNAAYPISFARRNE"
     gene            1323..1743
                     /gene="rnpB"
                     /locus_tag="VNR65_03440"
     ncRNA           1323..1743
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VNR65_03440"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          420 a          577 c          669 g          409 t
ORIGIN      
        1 atgcaagtgg ctaaaacgtc aaagaaaacc gatcccaagc tttgggaaaa agtgaaggga
       61 gatgtgaccg ccggcgataa gggcggccgt ccgggacagt ggtcggcgcg caaaagggcg
      121 gcggctatgt cggccccaag agcgaagaca actcgctcct gaactggggc acgatccgct
      181 gaagaattag cgctgccacc gttattcatt tcgtcgcgca aacgaaatcg gataggccgc
      241 gttgcggatg tcgatcatgg gatcgtcgga ctgctcgatc ccgtccagga gctgaccggg
      301 gagaaacagc aacttattct gggcctcgaa aggatcggcg gacactttgc tgatggtcaa
      361 aacgcccatg tccaccactt tgcggtcatc cggccacgcc ttcgacccat cgttggtttg
      421 gtcgcccggc gcggcgagtt gcgccttcag gtggaatgtg atcgggcctt tcttgagccg
      481 ctcctgcacc tcgtcgatga ggaaatatgg cgctttcttc tgagcttcgg cgggatcgag
      541 gtgcacgacg cgctcgggca caaggtgata gcggaaggct tgcttttggc ccgtcttgtt
      601 cacgaacacg aaggcgttca cgccgcgata ttcctcgtgg gcgaagctgt ctggcgtgtg
      661 caccgcggca ttggctgcgg cgatgctggg gtgcgcggca acgaattgtt cgattttcgt
      721 gggtttgggc gcgtccggcg ggctcgcggc gatggccagg aacaggtcgc ggaaatcagc
      781 gccgttcgag acgggaaaga acttcagcgc attgatgacg agatcgctat cgcttccatc
      841 gggaaggtgg aacttgatcg cgatgccgtg cgggttggcg tcggggattc cgtccggaat
      901 attcgggatg cccgtggcat cggaaaagcg cgccgtcagc gggatttcgc ttccgtcgaa
      961 gatcaccgcc ttgcttaccg tggcggcttc gggcgtcgcc ttgaaactgc cctcgaccac
     1021 gatgcccttc gcatgattgg cccggaagcc tttatgcaca ccgaacacct tggtcatggc
     1081 gtccacgatc tgttccgcta tcggtttttc gtccgcagcg gccgagcctg accatacgag
     1141 gcatgatgcg ataatcgctg ctacagttct tctgtttgcc ataggacgct aactgccttt
     1201 gtttgtgatt cactccggca ggtaacagcg gaggcactgc agaaggttcc gtggcaacaa
     1261 tgcccgtgca ttttttcagc cgccacttca cgcggcgcgc ccttcacccc ttactcaaat
     1321 tgccagttgg ccggatggcc gctgctcgaa aggcgcgttg tgggccgcaa ggtcgtagcg
     1381 cttttcgggt ggaggaaagt ccgggctcca cggaaacacg gcgccggata acgtccggcg
     1441 ggggcgaccc cagggaaagt gccacagaaa gcaaaccgcc cgcgttcgcg agggtaaggg
     1501 tgaaagggtg cggtaagagc gcaccgcagc gctggtaaca gggctggcaa ggcaaacccc
     1561 gccgggagca aaaccgaata gggacggcac agcttcgcgg cttcggctgc ggggcggaat
     1621 gcgttttcgc atcgccgtcc gggttggttg cttgaggcgc gcggcgacgc gcgtcccaga
     1681 tgaatggcca tcgcggtgga gcgatccgtc gcacagaacc cggcttacag gccaactggc
     1741 atctaatacc gtcgcaccga aagtagactc ctgttgcgct gctgcaatcc gtaggtgggt
     1801 cggatcgtga ttcgctatcg gtatgaatcg gaggggggac attatgacgg cacttgcgat
     1861 aagaggtgat cgcgcggcaa aaggcgaggc caagccatcc tcaaaaagac ccagcccaag
     1921 ctgaggctct taaaaggggc tccggcgctt ctggagaaaa ttcagtgtac gcacaaaaga
     1981 taagcgcctt cgcctgttct tccaggacga ggcgcgcatc tgccagaaag gccgcgtttg
     2041 ccatatctag tggaagcgcg acgagcgcga taggc
//
LOCUS       DATMGX010000211         2649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_368133, whole genome shotgun sequence.
ACCESSION   DATMGX010000211 DATMGX010000000
VERSION     DATMGX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368133"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VNR65_03445"
     CDS             <1..213
                     /locus_tag="VNR65_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWJ17763.1"
                     /translation="GLIVTGYGFGTVGNIVVGILGALLGGALLPRLGLFTGGDLVGQI
                     ISATLGAVLLLFLIGLVRRSGTPSRL"
     gene            587..946
                     /locus_tag="VNR65_03450"
     CDS             587..946
                     /locus_tag="VNR65_03450"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ17764.1"
                     /translation="MIRAVTLAAALAAVITLPAIAQDVKSPAATAQTAVTEQEAKTWV
                     DKPVYSSDGKKLGEVAAILRTADNKITELRADIGGFLGLGQHEISVPAARFMLQNDRV
                     TLDMTAAQAKELPKVAK"
     gene            1007..1204
                     /locus_tag="VNR65_03455"
     CDS             1007..1204
                     /locus_tag="VNR65_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWJ17765.1"
                     /translation="MNWDQIAGDWKQFEGRVQKQWGKLTNDDLQQAKGDQRILAGKIQ
                     ERYGITKEEAERQIKTWHDSL"
     gene            1280..1513
                     /locus_tag="VNR65_03460"
     CDS             1280..1513
                     /locus_tag="VNR65_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17766.1"
                     /translation="MAKEASHTGHPAHGHHTKAAEHHEQATKHHKEAAAHYASGHHEK
                     AAEHAHTAHAHALHAAHHAGEAAKSHVAHGPKK"
     gene            1625..1822
                     /locus_tag="VNR65_03465"
     CDS             1625..1822
                     /locus_tag="VNR65_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWJ17767.1"
                     /translation="MSWDQISGDWKQFEGKVQKQWGKLTNDDLQQAKGDQKILAGKIQ
                     ERYGITKEEADRQVKDWLNSH"
     gene            complement(2048..>2649)
                     /locus_tag="VNR65_03470"
     CDS             complement(2048..>2649)
                     /locus_tag="VNR65_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWJ17768.1"
                     /translation="AMLAGLVSYEIAYLAVVFGSAGILWFHHRATLPLFVGVGIFAII
                     TVAIPSAILGLRRLGKRRLIAWMDRLLGTTAAFRALAEAPADLLRSPVLLAQTVGLQL
                     GIFLLDALTLWLVFNAIGDVPEIWVVFVGFVMASMAATIGPMPIGLGTFEAGAVGMLH
                     FLGVSIEAALAATLLLRGLTFWIPMIPGVWLARREIGRL"
BASE COUNT          672 a          707 c          743 g          527 t
ORIGIN      
        1 ggcctaattg ttacgggcta tgggttcgga acagttggaa atatcgtcgt ggggatcctt
       61 ggcgcattat tgggaggggc gctattaccg cggctgggcc tgtttactgg cggcgaccta
      121 gtcggacaga ttattagcgc aacgcttggc gcggtccttt tgctcttcct tatcgggctc
      181 gtccggagat ccggaacgcc ctcgcgcctc tgatccgggg ttggcgtttg aaaaaaatgg
      241 gtgccgataa ttcggcaccc atttgaggat gaaatcgtag atctcgacac aaaacttttc
      301 acggatttct tcgcttcttg cgcgttgatc tattcaagac attgtgacat cggaggctcc
      361 gcacagaatt taaaagcgag ttctgatttg agccatgtta gattacagaa attgacggcg
      421 agtttggcgt cgatcgattg tgagatatac gggattttta ctagcctgat aaatttattg
      481 tgttgacgaa agttttcatc acatcttttg ataactatca ataacagtct tggtttacga
      541 catatacttt atacattaac gtttttttca gtgaggagta caaatcatga tacgcgcagt
      601 aacccttgcg gctgctcttg ccgcagtaat cacgcttcct gcaatagctc aggacgtcaa
      661 atctcccgcc gctaccgcgc aaacggcggt tacggagcaa gaggccaaga cctgggtcga
      721 caaacccgtg tatagcagcg acggtaagaa actcggtgag gtcgcggcaa tcctacggac
      781 cgctgacaac aagataaccg aattgcgcgc cgatatcggc ggcttcttgg gcctcggcca
      841 gcatgaaatc tctgttcctg cggcccggtt tatgctgcag aatgaccgcg ttacacttga
      901 catgacggcg gctcaggcaa aggaacttcc gaaggttgcg aagtaaggtt ccaaatttag
      961 gcattcgctc aagtgttcgt gcactgtttt aaaagggaga agagtcatga attgggatca
     1021 gatagccggt gactggaagc aattcgaagg tagagtacag aaacagtggg gaaaactcac
     1081 gaatgatgat ctgcagcaag cgaaaggcga ccagagaata ttggcgggca aaatacagga
     1141 acgctacggc attacaaaag aggaagctga gcgtcaaata aagacttggc acgactcgct
     1201 ctaacttcca ccaagcgcgt gggggcccca tccttggggc cccttttttt agtccaaaac
     1261 ccatcaagga agaacacaca tggctaagga agcatcgcac accggtcatc cggctcatgg
     1321 tcatcacacc aaggctgccg agcatcatga gcaggctacg aagcatcaca aggaagcagc
     1381 cgcgcactac gcttccggac atcatgagaa ggcggctgag cacgcgcaca ccgcccatgc
     1441 ccacgcgttg catgcggctc accatgctgg cgaagccgcc aagtctcacg tagcgcacgg
     1501 tccgaagaag taatctttct cgttagacag cctttgtttc aataaacgag gagttcaagt
     1561 tatgaagcgt acgctcacac ttgccgccat tctggccgcc gctatcacgc tgccagcaat
     1621 ggctctgagt tgggatcaaa tatccggtga ctggaagcag ttcgagggta aagtgcagaa
     1681 acagtgggga aaactcacca acgatgatct gcagcaagcg aaaggcgacc agaaaatatt
     1741 ggcgggcaag atacaggaac gttacggcat tacgaaagag gaagccgatc gccaggtcaa
     1801 agattggctc aactcgcatt agcacttatt cgtggtgaaa taagaggctc gaactttgcg
     1861 ggcctctttt cgctcagtct caatcccatc gcgcggaggt acggggaaga cttgagtttc
     1921 cttgacaaac cctacgccct ttttctcaag ccatgaggcg ggcagcgcag acggcggtct
     1981 acctgttccc acccggcacc atcgccgctc ggccggtgtc tccctgaaca gaacgagagt
     2041 gccgaggtca caagcgaccg atttcacgcc gggcgagcca aacacctggg atcatcggta
     2101 tccagaacgt cagcccgcgc agcagaagcg ttgcggccag agcggcctcg atggacaccc
     2161 cgaggaagtg gagcatgccc accgcgcccg cttcgaaggt ccccaggcca atcggcatcg
     2221 gtccgatcgt ggccgccatc gaggccatga cgaagccgac gaaaaccacc cagatctccg
     2281 gcacatcacc aatggcgttg aacacgagcc aaagcgtaag agcgtcgagc aggaagatcc
     2341 caagttggag gcccactgtc tgggcgagca agacggggct gcggaggaga tcggcgggcg
     2401 cctcggcaag ggcccgaaac gcggcggtcg tgccgagcaa cctatccatc caggcgatca
     2461 gccgccgttt gcccaaccgc cgcaagccga ggatagcgga gggaatcgca accgtaatga
     2521 tcgcgaaaat gccgaccccg acaaagagcg gcagagtggc gcggtggtgg aaccacaaaa
     2581 ttccggcgct gccgaacacg accgccagat aggcgatttc gtaggagacg aggcccgcca
     2641 gcatggccg
//
LOCUS       DATMGX010000212         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_395127, whole genome shotgun sequence.
ACCESSION   DATMGX010000212 DATMGX010000000
VERSION     DATMGX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395127"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..235)
                     /locus_tag="VNR65_03475"
     tRNA            complement(161..235)
                     /locus_tag="VNR65_03475"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(200..202),aa:Glu,seq:ttc)
     gene            complement(324..>2215)
                     /gene="recJ"
                     /locus_tag="VNR65_03480"
     CDS             complement(324..>2215)
                     /gene="recJ"
                     /locus_tag="VNR65_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HWJ17769.1"
                     /translation="RFTQDESDTLMLQKYWSQPLRSLSLPLPQPSRGSPAFLDVERSA
                     RGLRWVERLAPSQAYLGAAIAQRHNLPEIIGRLLAARDIALDQVPDVMNPTIRTLCPD
                     PATLTDMEKAAERIGHAVLAGESVAIFGDYDVDGASSSALLCRFLAWHGLDARIYIPD
                     RITEGYGPNKAAIASLIEDGAKLIVTVDCGSTNFDALEIAAKKSVDVVVADHHQVGEN
                     LPPAHAIVNPNRQDCLSGQGHLCAAGVVFLICVATQRVLRAKGFYAKRKAPDLLEWLD
                     IVALATVCDVVPLIGVNRAFVVQGLKVLRGRQNAGLKALCDAAGVNKPPSPYTLGFVL
                     GPRINAGGRIGDSGLGARLLASDDPTETARIAETLDKLNRERREIELKTVEEATAWAD
                     RALLEDPSCPIIIAGSPAWHKGLVGLAASRLTERFQRPCLIFSQDERAGEATGSARSV
                     SGVDIGAAVRGAVEAGVAKKGGGHAMAAGVTIEQHRLHELAAYLREALGPSYRAAAAA
                     PVLPVDGALTPRAATLEFAVQLESAGPYGQGNPAPRFCLPSVRLTMVKEITGGHLRCA
                     LQAVDGSQISAVAFRAANSDIGAALAQGRDLACHVAGRIERDEWGGKSRIEFQIEDVA
                     AAAKR"
BASE COUNT          448 a          690 c          694 g          383 t
ORIGIN      
        1 cgtttgacag cggaaatcgt gggctgtgaa caagcgaaaa aggcgcagag ccaagggctt
       61 atgcgcccgt ggaagagaat ggctcaacac agggatacga ttcctaaaaa agcccaaccc
      121 atgaaaaaac agacaaaatc aatgacttgg agcagatttt tggcgccccc taggggagtc
      181 gaacccctct ttcaagattg aaaatcttgc gtcctaaccg atagacgaag ggggcgcttg
      241 cggctgtgct agccgacagg aggatataag tcgggtttgt gcagcttggc aagcctgcaa
      301 tctcggaaat ttgccgttgg cgctcagcgt ttggcggcgg cggcaacatc ctcgatctgg
      361 aactcgatgc ggctttttcc gccccactcg tcgcgctcga tgcgtcccgc cacgtggcag
      421 gcgagatcgc gcccttgcgc gagcgcagct ccaatatcag agttcgcggc ccggaaggcg
      481 acggcgctga tctggcttcc atccacggcc tgcagcgcgc agcgaagatg cccccccgtg
      541 atctccttca ccatcgtgag cctgaccgaa ggcaggcaga atcgcggcgc gggatttccc
      601 tggccgtaag gcccggcgct ctcaagctgc accgcaaatt cgagtgtcgc ggcacgcggc
      661 gtcagcgctc cgtcgacggg tagcaccggc gccgcagctg ccgcccgata cgaaggcccg
      721 agcgcctccc gcaaatacgc ggccagctcg tgaagccgat gctgctcaat agtgacgccg
      781 gcggccatgg cgtggccgcc gcccttcttc gcgacgcccg cttcaaccgc gccccgcacg
      841 gccgccccga tatcgacgcc gctgacggaa cgcgccgaac ccgtggcttc gcccgcgcgc
      901 tcgtcctgcg agaaaatgag gcaagggcgt tgaaaacgct cggtcaggcg gcttgcagcg
      961 agcccgacaa ggcccttatg ccaggcaggc gagcctgcga tgatgatggg gcaagacgga
     1021 tcttccagca aggcgcggtc agcccaggct gtcgcttcct cgacggtttt tagctcaatc
     1081 tctcggcgtt cgcggttcag cttgtccagc gtttcagcga tgcgagccgt ttcggtgggg
     1141 tcgtccgagg cgagaagcct cgcaccaagc cccgagtcgc caatgcgccc gcctgcgttg
     1201 atccgggggc cgagcacgaa gccgagcgtg tagggcgaag gcggcttgtt cacgcctgcg
     1261 gcatcgcaaa gcgccttgag gcccgcattc tgacgaccgc ggagcacttt gagcccctgc
     1321 accacgaagg cgcgattcac accgatcagc ggcaccacgt cgcaaacggt cgccaaggcc
     1381 acgatgtcga gccactccag gaggtccggc gcctttcgct tcgcataaaa gcctttggct
     1441 cgcaaaaccc gctgggtggc gacgcatatc aggaagacaa cgcccgcggc gcagagatgg
     1501 ccttgcccgc tcaggcaatc ctggcggttg gggttgacga tggcgtgggc gggcggaagg
     1561 ttttcgccga cctgatgatg gtcggcaacc acaacatcca cgctcttctt ggcggcaatt
     1621 tcgagcgcat cgaagttcgt agagccgcag tcgaccgtca caatcagttt ggcgccgtct
     1681 tcaatcagcg aggcgatcgc cgccttgttt gggccatagc cttcggtgat ccgatcggga
     1741 atgtagatgc gagcatcgag accatgccag gcaaggaagc ggcacagaag ggcggacgac
     1801 gaagcgccat cgacatcgta atccccgaaa attgcgacac tttcaccggc tagaacggcg
     1861 tggccgatgc gctcggctgc cttttccata tcggtaaggg tcgcgggatc ggggcatagc
     1921 gtgcggatcg tcgggttcat gacatcggga acctggtcca gcgcgatgtc gcgcgcggca
     1981 aggagccgcc cgatgatctc aggcaaatta tgacgctgcg cgattgccgc gccgagataa
     2041 gcttgcgacg gcgcaagccg ctcaacccat ctgagaccgc gagcagaccg ctccacatcg
     2101 agaaaagccg ggctgccacg cgacggctgc ggaagcggca aggatagaga acgcaatggc
     2161 tggctccaat acttttggag catcagcgta tctgattcgt cttgcgtgaa cctcg
//
LOCUS       DATMGX010000213         4629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_46286, whole genome shotgun sequence.
ACCESSION   DATMGX010000213 DATMGX010000000
VERSION     DATMGX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46286"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..1943
                     /locus_tag="VNR65_03485"
     CDS             396..1943
                     /locus_tag="VNR65_03485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ17770.1"
                     /translation="MKSWNSGYLSEIDYTYGYYREISPVFLSLALLHRGVKQNATRPP
                     RYLELGFGQGLSVNIHAAACPGEFWGTDFNPAQATNAKELAIASGSDAKVFDASFAEL
                     AARADLPEFDIITLHGIWSWISEENRAAIMDLARRKLAVGGIFYVSYNCTPGWSSTMP
                     LRHLMTLHAEFAGFDDQGITGKVDGAVSFAKRVVDSGAAYFKANPGVADRLSNISKQN
                     RRYVAHEYFNGHWTPLPFSQVAGKLADAKLDFATSSNLLEHMDSVNLTKEGQTLLAGI
                     AHPIFRESVRDYVLNQQFRRDIYVRGRRQMTAFEQLEQYKLQRFVLLKAADKIALKAN
                     GPAGEITMQEEVYKPLIEILASRNYEPKSVAEIVAHPSWKSRSLTTAIEVLIVLTATG
                     QANPTQSEEVINQARPKCAALNAHICQRALFEGEIGYLASPVIGGGVTVDRLEQLFLL
                     AREAGHSNAKAWASCAWDALDSQGHKILRDGKPLAKAEENMAELRERAKAFEADRLPI
                     LSALGIS"
     gene            complement(2287..3417)
                     /locus_tag="VNR65_03490"
     CDS             complement(2287..3417)
                     /locus_tag="VNR65_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ17771.1"
                     /translation="MRIFCGAIGVSFLMSGAVLADTNGDCAQDANAELAIRACTAIIQ
                     DTRGSNAVAYFSRGRAYDSKGDRVKASDDFDEAIRLSPQYTNAYAGRGFVYLHRGLFG
                     RAITDFSQALKLDPKYKEAFFGRGLTFLAVSDNGRATEDFGKAIALDPKYEPAYIKRG
                     TAYGSDGQWDRAIADYNTAISLNPANADSYDARGVAYSEGKGDYSHAVADFDKAISLD
                     PAHASAYVNRGLAHQRKGDNAQAVADYDKAISLNQDSASAYNSRALLLYATEQAREAK
                     ADADKAVSLKPNDWRFLDTRGHILLALGDTNAALEDFNAALRINGDCISSLWGRGQVY
                     EAQELPALAILDFKKAIELKASVSANREAQTKARARLAVLAR"
     gene            3608..>4629
                     /locus_tag="VNR65_03495"
     CDS             3608..>4629
                     /locus_tag="VNR65_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17772.1"
                     /translation="MKRIITVAALASLPMMAEANELPLKRVVLSTSGLAQFTHGGEAA
                     AGSVIELPVRLDQVDDVLKSLTIFDAAGSIGAVSLPGKTPLAELFRDLPFDQAALASQ
                     PDLLNALIGAEVEIEGSVSATGRVFRVEEEKVQLPNNGGETTRHRLTLVTSKGFVQAI
                     LEELTALRFTDAATRAQIDRALSGIATNRAKDQRKLSITLAGEGKRPAGFSYVVAAPV
                     WKTSYRVVLPKEGGKARLQGWGILENLTGGDWKDVDLTLVSGNPVALKQALYSAVFVD
                     RPEIPVSSTLRFVPRKDEAEEKARDMTEAMAPRAKAGRALRAAPPSPVAASPAFGAGG
                     FPGALQ"
BASE COUNT          977 a         1273 c         1351 g         1028 t
ORIGIN      
        1 ggattgtggc cggtgacctc ataacggccc atggcaagcg atggggccgt tggggcaacg
       61 ctcgcgaaga gttcaagccg ctcgttcgct ttcccgtccg cccattcgcc attgagaaag
      121 ccgtgcttgc cgaatgtgta aatgaccgga tggatttctc cccagttcac gatgagcaaa
      181 gatagcgccg gcgggcggct ggctcagttt cagtggattt tacacaacta aaggttgaaa
      241 tgtgcatgct agggaaatag tgttgcttgt gctgccgttc gggtgccggt gttccaaggg
      301 ccttatatgc aaggcaaaaa agggctttcg aaagcaaacc ttcgcttatc ctccaccgac
      361 cgaacggctt taattgaggc tgaacgagaa aaaatatgaa gagctggaat tcaggctatc
      421 tatctgaaat tgattatacg tacggatatt atagggaaat atctcccgta ttcttgtcgc
      481 ttgctcttct tcataggggc gtaaagcaaa atgccacccg cccccctcga tatcttgagc
      541 tgggatttgg tcaaggactc tccgtaaaca ttcacgccgc cgcttgccca ggcgagtttt
      601 ggggaacgga tttcaacccg gcacaggcca caaacgctaa agaactcgca atcgcttctg
      661 gctcagatgc aaaggtcttt gacgcgtcgt ttgctgaact tgctgcccgg gcggacctgc
      721 cggagttcga tatcatcact ttgcacggga tttggtcctg gatttctgaa gagaatcgcg
      781 ccgcgatcat ggacctcgcc cgtcgcaagc tggctgtggg tggaatattc tatgtaagct
      841 acaattgtac accgggctgg tcgtcgacga tgccgttgcg ccacctcatg acgcttcacg
      901 cggagttcgc agggtttgac gaccagggta ttaccggtaa agtcgacggc gcagtatcgt
      961 ttgctaaacg cgtcgtcgat tccggtgctg cgtattttaa agccaaccca ggcgttgctg
     1021 atcgtttaag caacatctca aaacagaaca gacgctatgt tgcccacgag tattttaatg
     1081 gccattggac gccgctaccc ttctcgcagg tcgcagggaa acttgcagac gcaaaactgg
     1141 attttgcaac gtcctcaaac cttctggagc atatggatag cgtcaatctc actaaggagg
     1201 ggcagaccct gctcgctggg attgctcacc ccatctttcg agagtcagtg cgagattatg
     1261 tcctgaatca gcagttccgg cgtgatattt atgttcgtgg acgccgtcaa atgactgcct
     1321 tcgagcagtt ggagcaatat aagctccaac gcttcgtgct gttgaaggcc gccgataaga
     1381 ttgcgctgaa agccaatggc ccagcgggcg agatcacgat gcaggaggag gtttacaagc
     1441 ctctcatcga gatattggca agtcgtaact acgaaccgaa atcggttgcc gagatagtag
     1501 ctcacccgtc ttggaaatcc agatcgctga ccaccgcgat cgaggtccta atagtcttga
     1561 ccgcgaccgg ccaggccaat ccaacccaat ctgaggaggt catcaatcaa gcacgcccaa
     1621 agtgcgctgc gctgaacgca catatctgtc agcgcgcact atttgaaggg gagatcggat
     1681 atttggcgtc gcctgttatc ggaggcggag ttaccgtcga tcgcctggaa cagcttttcc
     1741 ttcttgcccg agaagctggg cattctaacg cgaaagcctg ggcttcttgt gcatgggatg
     1801 ctttggattc tcaaggccac aagatactga gagatggcaa gcctctcgcg aaagcggaag
     1861 aaaatatggc tgagctgaga gagcgagcga aggcatttga ggcagatcgc ctgcccattt
     1921 tgagtgcgct tggaattagt tagtaatgcc ttggacggcc gcggcctcgg cgtgaggttt
     1981 cccgtgttga tcatgcacgg cagagggggc cgtttattcg tcccggtgag ggtgatgatg
     2041 aacagcgata gatccgatcc ctgccgaatg cgaaagggca gggcagcgct caaaggtaga
     2101 tggacgtagc gtttctagcg cccggtcccc attaacgtca ccctgtctgg caatcgaagc
     2161 gcgagccggg gtcgcaaaga gtgacgcaat tcaatttgtt aaggtcccgg ataaggcttc
     2221 gcctttccgg gacgacgcag tgcggagcga gcggcttagc tgaagcgcgt ccgctgaacc
     2281 gacgccttac ctggccaata ctgccagccg cgcgcgtgct ttcgtctgcg cctcgcggtt
     2341 ggcgctcacc gaggctttca gttcaattgc cttcttgaag tccaagatgg cgagcgccgg
     2401 aagctcttgt gcctcgtaga cttgcccacg gccccaaagc gagctgatgc agtcgccatt
     2461 gatgcggagc gcggcattga agtcctccaa cgctgcattc gtgtctccaa gcgccagcag
     2521 gatatgtcct ctcgtatcga ggaagcgcca atcgttcggc ttgagcgaga cggccttatc
     2581 ggcatccgcc ttcgcttccc tggcctgctc cgtcgcatac agcaacaggg cgcggctgtt
     2641 ataggcgctc gcactatcct ggttcaggct gatagcctta tcgtagtcgg caacggcttg
     2701 agcattgtca cccttgcgct gatgggcgag gccgcggttg acataagcgg acgcgtgcgc
     2761 ggggtccagg cttatggctt tgtcgaaatc ggcaacggcg tggctataat cgccttttcc
     2821 ttcgctatag gcgacgccgc gagcatcata ggagtcggcg ttcgcagggt tcaaactgat
     2881 agctgtgttg taatcggcaa tcgcgcggtc ccattgcccg tcgctgccat aagcagtccc
     2941 gcgcttgata taggccggtt cgtatttcgg atcgagcgcg atcgccttgc cgaaatcctc
     3001 cgtggcgcgt ccgttatcgg acacagcgag gaacgtcagc ccccggccga aaaaggcttc
     3061 cttatatttt gggtcgagct tgagcgcctg actgaaatct gtgatggcgc gcccgaacag
     3121 tcctcgatgg agataaacga aaccgcgtcc cgcataagca ttggtgtatt gcgggctgag
     3181 cctgatggcc tcgtcgaaat catcgcttgc cttgacacga tcgcccttgc tgtcataggc
     3241 gcgcccccgg ctgaaataag cgactgcgtt gctacccctg gtgtcttgga tgatcgcggt
     3301 gcaggcgcgg atggcaagct cggcattagc gtcctgggcg cagtcgccat ttgtgtcggc
     3361 aaggaccgcg ccggacatga ggaacgagac gcctatggcg ccgcagaaaa tgcgcatgaa
     3421 aacctcccta tgtatatttt cttttgattg aatatatagc gttatgaatt taagcgccag
     3481 ccatgctgct agagttattc atataatacc gaattgagcg ccggatttag ccgcagtcgc
     3541 accgcaattg cgcttttatt caagccgctg gttgctaatg ttttcaatct ctttcggatc
     3601 gaacgtcatg aaacggatta tcacggtagc cgccctggcc agtcttccta tgatggccga
     3661 ggcaaacgaa ctgcccttga agcgcgtcgt cctttcgacg tcagggcttg ctcaattcac
     3721 gcatggagga gaagccgccg ccggatctgt catcgagctg ccggtccgcc tcgatcaggt
     3781 cgacgacgtg ctcaaaagcc tgacgatttt cgacgcggcg ggctccatcg gcgccgtcag
     3841 cctgcctgga aaaacccctc tcgccgagtt gtttcgcgat cttccattcg atcaggctgc
     3901 gctcgcctcg caacctgatt tgctgaacgc ccttatagga gccgaggtgg aaatcgaagg
     3961 cagcgttagc gcgacgggcc gtgtcttccg cgtcgaggag gagaaagtgc agcttccgaa
     4021 taatggcggg gagacgactc ggcacaggct cacgcttgtt acctcgaaag gcttcgtcca
     4081 ggccattctc gaagagctga cggccctgcg cttcaccgat gccgcgacac gcgcccagat
     4141 cgaccgcgcc ttgtcgggga tcgcgaccaa tcgcgcaaag gatcagcgca agctctcgat
     4201 cacgcttgcg ggcgaaggga aaagacccgc cggcttcagc tatgttgtgg cagcacccgt
     4261 ctggaagaca tcctacagag tcgtgctgcc caaggaaggt ggaaaggccc ggcttcaggg
     4321 ctggggcatt ctggaaaatc ttaccggcgg cgattggaaa gacgtcgatc tcacgcttgt
     4381 ttcgggcaat ccggtggcgt tgaagcaagc gctttattcg gcggtcttcg tggaccggcc
     4441 cgagatcccg gtgtcctcta cgttgcggtt tgtgccgagg aaggacgagg ccgaggagaa
     4501 ggcccgcgac atgacagagg caatggcccc ccgcgcgaaa gcgggacgtg cgctccgggc
     4561 ggcgccgccg agccctgtag cggcatctcc ggccttcgga gcgggcggct ttccgggggc
     4621 cctccagga
//
LOCUS       DATMGX010000214         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_357137, whole genome shotgun sequence.
ACCESSION   DATMGX010000214 DATMGX010000000
VERSION     DATMGX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357137"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VNR65_03500"
     CDS             <1..1676
                     /locus_tag="VNR65_03500"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17773.1"
                     /translation="RLKPQCFYDLVIEVAIVRPGPIQGDMVHPYLRRRQGIEKATFPS
                     EELKGILEKTLGVPLFQEQCMKIAIVAAGFSPSRADELRRAMATFKKVGTIGQFKDEF
                     INGMLAKGYEREFAERCFSQIQGFGTYGFPESHAASFALLVYCSAWMKCYYPDVFAAA
                     ILNSQPMGFYAPAQLVRDAREHFVEVRPVDVNASDWDHTLEALDPLSCPSPGELAPAS
                     SHLSMTASASGCGGGEGTPLHPARSAPSPLAGEGKLPQKHNHALRLGLRLIAGMRKDD
                     GKSIMEARKTGGAFKSPEDLMRRAKLKPSAMLTLARADAFGSLDFGRRETLWTVAGLE
                     TNELPLFRPGDATERTGNIHNAKPFICNESGGLRSRLRDDDGIGLPPLKQEEVVAEDY
                     AVFGLSLRNHPMAFMREEMCAKGMITAADLKKLANGKFVKIAGLVLFRQRPGTAKGTI
                     FITMEDETGAANLIVWPKIAETYRRAVFGAKVILCEGILQKESNVIHVVSRRLSDWTP
                     MLRRLQPEASAFGLRFGRGDEVAHASGGNARTSRANTSWTDTLKSRDFR"
     gene            1801..>2062
                     /locus_tag="VNR65_03505"
     CDS             1801..>2062
                     /locus_tag="VNR65_03505"
                     /inference="COORDINATES: protein motif:HMM:NF019019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HWJ17774.1"
                     /translation="MRVKSHIWVSAYLRRVNSAFVHAALVRRGDADAGAIYIKVAAMD
                     GTAQVFAPLSAELAEMTPSASLIMEEGRAWQPVYSPHAREAEA"
BASE COUNT          423 a          606 c          632 g          401 t
ORIGIN      
        1 cgaggctcaa accgcaatgc ttttacgatc tcgtcatcga ggttgccatc gtccgccccg
       61 gcccgatcca gggagacatg gtgcatccct atctgcgccg ccgccaggga atcgaaaaag
      121 ccactttccc aagcgaggag ctgaaaggca ttctcgaaaa gacgctcggc gtgccgctct
      181 tccaggagca gtgcatgaaa atcgccatcg tggcggcggg cttttcgccg agccgcgccg
      241 acgagttgcg ccgcgccatg gccaccttca agaaggtcgg caccatcggc cagttcaagg
      301 acgagttcat caacggcatg ctcgcgaagg gctatgaacg cgaattcgcc gagcgttgct
      361 tcagccagat ccaggggttc ggcacctatg gctttcccga gagccacgcg gcttcattcg
      421 cgctgctcgt ttactgctct gcctggatga aatgctatta ccctgacgtg ttcgccgccg
      481 cgatcctgaa cagccagccc atgggctttt atgcgccggc gcagctggtg cgcgatgcgc
      541 gtgagcattt tgtcgaggtg cgccccgtcg atgtgaatgc gagcgactgg gatcatacgc
      601 tggaagctct tgacccctta tcctgtcctt ctcccggcga actcgccccg gcgagttcgc
      661 atctcagcat gaccgcatcc gcaagcggat gcggtggggg agaagggacg ccgttgcacc
      721 ccgctcggtc tgctccctct ccccttgcgg gagaggggaa actccctcaa aaacacaatc
      781 atgcgctgcg tctcggcctt cggctgatcg caggcatgcg caaagacgac ggcaaaagca
      841 tcatggaagc gaggaaaaca ggcggcgcct tcaaaagccc ggaagacctg atgcgccgcg
      901 cgaaactcaa gcccagcgcc atgctcacgc tcgcccgcgc agatgctttc ggctcgctcg
      961 atttcggccg ccgcgaaacg ctctggactg tcgcaggtct tgagaccaac gaactgccgc
     1021 ttttcagacc cggagacgcc actgaaagga ccgggaacat tcacaatgcc aaaccattca
     1081 tttgtaacga gtccggcggc ctgcgaagcc ggcttcggga tgacgatggc atcggactcc
     1141 cgcctttgaa gcaggaagag gtggttgcgg aagactatgc ggtgttcggc ctttcgctca
     1201 ggaaccatcc catggcgttc atgcgcgagg agatgtgcgc caaggggatg attacggcag
     1261 cggacctgaa aaagctggcc aatggaaaat tcgtgaaaat tgcgggcctt gtgctgttcc
     1321 gccagcggcc gggaaccgcg aaagggacaa tctttataac gatggaggac gagacgggcg
     1381 cagcaaatct gatcgtatgg ccgaagatag ccgaaacata ccgtagagcc gtttttggag
     1441 cgaaggtgat tttatgcgag ggtatcttgc aaaaagagag taatgtgatc cacgtcgtca
     1501 gccgccgtct tagcgactgg acccctatgc tgcggcgtct tcagcctgaa gccagcgcat
     1561 ttggattgcg gttcgggcgc ggcgacgaag ttgctcatgc atcaggcggc aatgcgagga
     1621 cttccagagc taacacgagt tggacggata ccttaaaaag ccgcgatttt cgataagacg
     1681 atctttcatt ctttcgggta gccgtggggc gccacagctg ccggaagtgg gtcacatccg
     1741 atcctgggcg aaggctgcgc tggcgggcgt ccgcttttgc ctgcatccaa agaggggagc
     1801 atgcgtgtaa agtcacatat ctgggtgagc gcctatctgc ggcgtgtcaa ctccgccttc
     1861 gtccatgccg ctctcgtgcg gcgcggtgat gccgacgccg gcgcgatcta tatcaaggtg
     1921 gcggccatgg acggtacggc tcaggttttc gctcccctat cagcggagct tgcggagatg
     1981 accccgagcg cctcgctgat catggaggaa ggccgggcct ggcagccggt gtattctccc
     2041 cacgcccggg aagcggaggc tg
//
LOCUS       DATMGX010000215         3512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_151919, whole genome shotgun sequence.
ACCESSION   DATMGX010000215 DATMGX010000000
VERSION     DATMGX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151919"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VNR65_03510"
     CDS             <1..424
                     /locus_tag="VNR65_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HWJ17775.1"
                     /translation="TDSFFLEYYSQVMHIVSNVAGRLGAKHDSIDALMAGFPAGTVSG
                     APKVRAMEIIDELEPEKRGVYAGCIGYFAANGEMDTCIALRTAIVKDGMMYVQAGAGI
                     VADSVPASEQAECENKARALFRAAEEAVRFAGQVSRGQ"
     gene            446..985
                     /locus_tag="VNR65_03515"
     CDS             446..985
                     /locus_tag="VNR65_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWJ17776.1"
                     /translation="MKTVTLFRHAKSGEKDNPRIEDFDRPLSDRGLKAAPKMGIAMRD
                     RHLRPNLVLCSPSVRTRQTLTLASAEAWDKAPKVRYDERLYEASAQNIFEVLKETPDK
                     VDHVMIVGHNPGMQDLAVMLAVNGKERQQLKEKLPTAALVSFEFDEKQWKELLPATGH
                     VVLYISPKTLPSAGQGQKQ"
     gene            982..1626
                     /locus_tag="VNR65_03520"
     CDS             982..1626
                     /locus_tag="VNR65_03520"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWJ17777.1"
                     /translation="MITIIDNYDSFTYNLVHYFGDLGVDVRVYRNDEKTAEAVIEEKP
                     EAIVMSPGPCTPNEAGICLDLVNENKGRIPLLGVCLGHQTIGQAFGGRVIRAPILMHG
                     KLSKIYHEGTSLFRGLPNAFEATRYHSLIVEADTLPGCLAVTARTSDGLIMALEHRGE
                     PTFGVQFHPESIASANGHAILANFLTLASVRWSPRKGGLVLPALEPAAGHATVA"
     gene            1635..2714
                     /gene="trpD"
                     /locus_tag="VNR65_03525"
     CDS             1635..2714
                     /gene="trpD"
                     /locus_tag="VNR65_03525"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HWJ17778.1"
                     /translation="MQDKPKPDIANSFRRHFEKAAGGACLSEDEAREAFTCIMQGAVS
                     EIELAGFLVALKARGETVDEIAGAVAAMRTLMVPATAPEGAIDIVGTGGDAKGTFNIS
                     TATSFVLAGAGVPVAKHGNRAVSSKSGAADVLEKLGVTIRMPSERIAHCFERAGLAFL
                     WAPTHHPAMRHAALVRQVLKLRTIFNLLGPLLNPAATKRMLIGAYSEQWLVPMAEVLK
                     RGGAEHVWAVHGAGGMDELSTTGVSRVVELKDGKLSSFEVHPSDAGLPEASLSDLQGG
                     SAEDCAVAMRELLRGKNGPFRDIVLLNAAAAFVIARRAGTLKEGACLAAASIDEGRAE
                     KALDALISASRDDTFAQPELVVDAK"
     gene            2832..>3512
                     /gene="trpC"
                     /locus_tag="VNR65_03530"
     CDS             2832..>3512
                     /gene="trpC"
                     /locus_tag="VNR65_03530"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWJ17779.1"
                     /translation="MSILDRIVAYKREEVAAAQAKISLAKLEEMASAGPCLRDFFGAL
                     SRKCARVEPGLIAEVKKASPSRGLIRADFDPAAIAKAYERGGAACLSVLTDGPSFQGS
                     PEALIAARGATRLPVLRKDFMVDPYQVAEARAMGADCILIIMACTSDGLAGELFSTAR
                     AMDMSVLVEVHNQEELERALMLNAWLIGINNRDLNTFETHLETTEELAQLIPPGRIIV
                     SESGIFTPA"
BASE COUNT          699 a         1019 c         1106 g          688 t
ORIGIN      
        1 caccgacagc tttttcctgg aatactacag ccaggtgatg cacatcgtct cgaacgtggc
       61 cgggcggctc ggtgcaaagc acgattccat cgacgcgctc atggcaggct ttcccgcggg
      121 caccgtatcc ggcgcgccga aagtccgcgc catggagatc attgacgaac tcgaacccga
      181 aaagcgcggc gtttacgcag gctgcatcgg atactttgct gccaacgggg agatggatac
      241 ctgcattgct cttcggacgg cgatcgttaa agatggcatg atgtacgttc aggccggtgc
      301 cggcatcgtc gctgattccg ttcccgcgtc ggagcaggcg gaatgcgaga acaaggcgcg
      361 ggcgttgttc agggctgccg aagaggcggt gcggtttgcg gggcaggtga gccgtggcca
      421 gtagcgcgga agatcaggtt gtccaatgaa gacggtgaca ctctttcgcc acgccaagtc
      481 cggtgagaag gacaatcctc gcatcgaaga tttcgatcgc cccttgtcgg atcgcggtct
      541 caaagccgcg ccgaaaatgg gcatagccat gcgtgatagg catttgcgtc ccaatctagt
      601 tttgtgctcg ccttcggtgc gcacgcgcca gacgctgacg ctggcctcgg cggaggcatg
      661 ggataaggcg ccgaaagttc gctacgacga gaggttgtac gaggcgagcg cccaaaacat
      721 tttcgaggtt ttgaaagaga caccggacaa agtggatcac gtcatgatcg tcggccacaa
      781 tcccggtatg caagaccttg cagtcatgct tgcggtaaac ggcaaagagc gccagcagct
      841 caaggagaag ctgccgacag cggctcttgt gagctttgaa ttcgacgaga agcagtggaa
      901 ggagttgctg cccgcgactg ggcatgtcgt gctgtacata tcgccgaaga cactcccgtc
      961 cgcgggccaa ggacaaaaac agtgattacg attatcgata actacgacag cttcacctac
     1021 aatctcgtcc attatttcgg cgatcttggc gtcgacgtgc gcgtctaccg taatgacgag
     1081 aagacggcgg aggcggtgat cgaggaaaag cccgaggcga tcgtcatgtc gccggggccg
     1141 tgcacgccga acgaggccgg catctgcctc gacctcgtga acgagaacaa aggacgcatt
     1201 ccgctgctgg gggtgtgcct ggggcaccag accatcggtc aggctttcgg cggccgtgtg
     1261 atccgcgcgc cgatcttgat gcacggcaag ctctcgaaaa tctaccacga gggaacgagc
     1321 ctctttcgcg gattgccgaa cgccttcgaa gcgacacgct accattcgct cattgtcgag
     1381 gcggataccc ttccgggttg cctcgcggtg acggcgcgca catccgatgg cctcatcatg
     1441 gcgcttgagc atcgcggcga gccgactttc ggcgtgcagt tccaccccga aagcatcgcg
     1501 agcgcgaacg gccacgcgat cctggcgaac ttccttacgc ttgcaagcgt gcgctggtcg
     1561 ccgcgaaaag gggggctcgt tctgccggcg cttgaacctg ccgccggaca cgcgactgtc
     1621 gcttgagcca ggccatgcaa gacaagccca agcccgacat cgctaactcg ttccgccgtc
     1681 atttcgagaa agccgcaggc ggagcctgcc tttccgagga tgaggcgcga gaggcattca
     1741 cctgcatcat gcagggggcg gtttccgaga tcgaactggc agggtttctc gtggcgttga
     1801 aggcgcgcgg tgaaacggtt gacgagatcg ccggcgcggt cgctgcgatg aggacactca
     1861 tggtgcccgc gaccgctccc gaaggcgcca tcgatatcgt tggcacgggc ggcgacgcca
     1921 aggggacgtt caacatctcg acggcgacgt cgttcgtcct ggctggggcg ggtgtgccgg
     1981 tagcgaagca cgggaacagg gccgtatctt cgaaatccgg cgcggcggat gtgctcgaaa
     2041 agctcggcgt cacgatccgg atgccttcag agcgcatcgc acactgtttc gagcgtgcgg
     2101 gactggcctt tctctgggcg cccacgcacc acccagcgat gcgccatgcg gccttggtgc
     2161 gccaggttct taaactgcgg acgattttca accttcttgg gccgctcctc aatcctgctg
     2221 ctacgaagcg catgctcatc ggcgcctatt cggaacaatg gcttgtcccg atggctgagg
     2281 tgctgaagcg gggtggcgcg gagcatgtgt gggccgttca tggggcgggc ggcatggacg
     2341 agctttcgac gaccggcgtc agccgcgtgg ttgaattgaa agacggcaag ctttcctctt
     2401 ttgaggttca ccccagcgac gccggcttgc ctgaagcgag cctgtccgat ctgcaaggtg
     2461 gttcagcaga ggattgcgcg gtcgcgatgc gagagctttt acgcggcaag aacggtccgt
     2521 tccgggacat cgttctcctg aatgccgccg ccgcattcgt catcgcgcgt cgagccggca
     2581 ccttgaaaga aggcgcatgc ctcgccgccg cttccatcga cgagggccgc gccgagaaag
     2641 cgctcgatgc tcttatttca gcaagcaggg atgacacctt tgcgcaacca gaattagtgg
     2701 tcgatgcaaa gtaatgttaa attgcaatct tcgaatgctc ttggtggaaa acacttgagc
     2761 gattgcggcg ttctgccatc tgatagtcgc cacaacccgt acgtaagagt gttagaacaa
     2821 acggtggcgc tatgagcatt ctcgaccgca ttgtcgccta taaaagggaa gaagtcgcgg
     2881 cagcccaggc gaagatctcc ctggccaagc ttgaggagat ggcttctgcg gggccgtgct
     2941 tgcgcgactt cttcggtgcg ctgtcgagaa aatgtgcccg cgtggagcca ggcctcattg
     3001 cggaagtcaa gaaggccagc ccttcgcgcg gtctgatccg ggccgatttt gatcccgccg
     3061 ctatcgcaaa agcctatgag cgtgggggag cagcttgcct ttccgtgctg acggatggtc
     3121 cctcttttca aggttcaccc gaggcgctga tcgcggcgcg cggggcgacg cgcctaccgg
     3181 tgcttcggaa agactttatg gtcgacccct atcaggtcgc cgaagcccgt gccatggggg
     3241 cagactgcat cctcatcatc atggcttgca catctgacgg gcttgccggc gagcttttct
     3301 ccaccgcgcg cgccatggac atgtcggtgc ttgtcgaggt tcataatcag gaagagctgg
     3361 agcgcgcgct tatgctgaac gcctggctga tcggcatcaa caatcgcgat ctcaacacct
     3421 tcgagacgca tctcgaaacg acagaagagc tggcgcaact gattccgccg ggtcgcatta
     3481 tcgtctcgga gagcggaatc ttcacgccgg cc
//
LOCUS       DATMGX010000216         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_201303, whole genome shotgun sequence.
ACCESSION   DATMGX010000216 DATMGX010000000
VERSION     DATMGX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201303"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VNR65_03535"
     CDS             <1..338
                     /locus_tag="VNR65_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17780.1"
                     /translation="NRLVAGELEARWNRALIGVAEVESGGDHLRIKRFYGTSENAVKT
                     QIWIAVSVYVLVAIVKKRLNLEASLYTLLQILSVTLFEKMPINQALVGGGPKYDNTQI
                     NNQLNLFAY"
     gene            950..2137
                     /locus_tag="VNR65_03540"
     CDS             950..2137
                     /locus_tag="VNR65_03540"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ17781.1"
                     /translation="MTAASEDFDAIILGAGAAGLMCALTAGQRGRKVLLLEHTDKAGA
                     KILISGGGRCNFTNLGVAPERFLSDNPHFCLSALRRYTQWDFISLVERHRISYHEKTL
                     GQLFCDRSARAIVAMLLQECAGGGVELRLGQSVTNISKPDSFRVETNSGVFTAPALVL
                     ATGGLSIPKMGATTFSHDAARRFGLRVTETRPGLVPFRARHDTPGLDPSLAGVSLEAS
                     VSCGTHTFREDILFTHRGLSGPAILQISSYWRPGISIAIDLLPGLHATGFLKERKRMR
                     PKALPQTILAEVLPSRLAQFLTAAHLPSGEMANIPDRTLAALAEQLNGWQFKPHETEG
                     FAKAEVTVGGIDTRDLSSKTMEARNVGGLYAIGEAVDVTGWLGGYNFQWAWSSGWCAG
                     QAI"
     gene            complement(2248..>2609)
                     /locus_tag="VNR65_03545"
     CDS             complement(2248..>2609)
                     /locus_tag="VNR65_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17782.1"
                     /translation="TAITPDYALGAHTASLGLTFSAGRKLPAPFMTGAFIGQHGSWNR
                     KPYSGYKVLFVPFENGKPKGDPVEVLTGFLSADEKAYGRPVGVTFDKSGALLVADDVG
                     NVIWRVTGQISRSAMRN"
BASE COUNT          550 a          738 c          747 g          574 t
ORIGIN      
        1 ctaaccgcct ggtcgccggc gaacttgaag cgcgatggaa ccgggcgctc attggtgtcg
       61 ccgaggtgga aagcggcggc gaccatctgc gcatcaaaag gttctacggc acgtcggaaa
      121 atgccgtaaa aacccaaatc tggatcgccg tctcggtcta cgtcctcgtc gccatcgtca
      181 aaaaaaggct caatctcgaa gcctccctct acacattgct ccagattctt tcggtcaccc
      241 tcttcgagaa aatgccgata aatcaagccc ttgtcggagg cggtcccaaa tacgataata
      301 cgcaaatcaa taaccaattg aatctattcg cttattagcc ggacagcagt ggagccacct
      361 ctaatctcga agccatcgcc agagttttga agggggaagg tgtcgaattg cggggggctt
      421 agactcgtga tgactcgttg aactgagaac gggcatgttc taatgccaag taggatcgct
      481 ttgaggctca agctgcctgg cgatccaagc ttgcgcctcg ctttggctgc tgaagcgtac
      541 ccatattttc cgatggcggt atatacccac tacaaacaca tgagacccat agctcttgat
      601 cagcagagct tcatattgac gatttccaac catgcaaggc aaaacctatt tatcgaccct
      661 gctagggtcc tagctgttcc gttttgtatt ttcaattaaa aaggacatgt ttattaattt
      721 ttaggaaata gctttcttat gaggaggtcc ttggtgcgtt tgctacataa ggccggagaa
      781 ctggcggtta gggccaagag cagccgaagc acccgtcccg acaatggcgt acgtcgtgca
      841 attgcagcac gtctcgcctg cgactatgaa agttggcgcg ctgagatgaa cgatccgggt
      901 ccgatgcgag atctgctgga gcgcgagcca attcatggaa agggcgcgcg tgacagcagc
      961 ctctgaagat ttcgacgcca tcattcttgg cgctggcgcg gcggggttga tgtgcgcgct
     1021 gaccgctggg cagcgtggcc gcaaggttct gctgcttgaa cacacggata aggcgggcgc
     1081 taaaatcctg atatcgggcg gggggcgctg taacttcacc aatctcgggg tcgcgcccga
     1141 acgcttctta tcggacaatc cgcatttttg cctctccgcg ctgcgccgct atacgcaatg
     1201 ggacttcatc agcctcgtcg agcgccaccg gatctcctac catgagaaga ctttgggcca
     1261 gttgttttgc gatcgttccg cgcgcgccat cgtcgccatg ctgctgcagg aatgcgccgg
     1321 cggcggagtc gaactgagat tggggcagag cgtcaccaat atttccaagc ccgacagttt
     1381 ccgggttgaa acgaactctg gcgtttttac ggcgcctgct ctggtgctgg cgacgggtgg
     1441 gctttccatt cctaaaatgg gggccacaac cttcagccat gatgcggctc ggcgctttgg
     1501 cctgcgagtc accgaaacac gcccgggact tgtgccgttc agggcgaggc atgacacgcc
     1561 cggtttagac ccatctctcg caggcgtatc cctggaagcg tccgtgtcct gcggcacgca
     1621 caccttccgc gaagacatcc ttttcaccca ccgtggattg tctggccctg cgattttgca
     1681 aatatcgtcg tattggcgtc ctggcatttc aatcgccatc gatcttctgc cgggtcttca
     1741 tgcaaccggg tttctcaagg agcgcaaacg catgcgtccc aaggccctgc cgcaaaccat
     1801 cctcgcggaa gtcctgccgt cgcgccttgc gcagttcttg accgcggctc accttccatc
     1861 aggcgagatg gccaatatac ctgaccggac attggcagcg ctcgcggagc aattgaacgg
     1921 ctggcagttc aagccgcacg agacggaagg cttcgccaaa gccgaggtta ccgttggagg
     1981 catcgatacg cgggatctgt catcgaagac catggaggct cgcaatgtgg gcgggcttta
     2041 cgccatcggc gaagccgtcg atgtcacggg atggctgggc ggatacaatt tccagtgggc
     2101 ctggtccagc ggctggtgcg cgggacaagc gatttaaaag atgcgctgtg cgatatctct
     2161 tgatagtcgc gccttgcagg agcgcgagct tactgagcca gtccccagag aaatacagcc
     2221 caagacgatt gacctttatg aggtgtctca gttccgcatc gcgcttcgcg agatttgtcc
     2281 tgtgacgcgc caaatcacgt ttccgacatc atccgccaca agaagggccc cagatttgtc
     2341 gaaggtcacg ccgacgggcc ggccgtaggc cttctcgtcg gcgctcaaaa agccggttag
     2401 cacctcgacg ggatcgccct ttggctttcc gttttcgaac gggacgaaaa gaaccttgta
     2461 gccgctatac ggcttgcggt tccacgagcc gtgctgaccg atgaaggctc cggtcatgaa
     2521 gggcgcgggc agtttgcgtc ctgccgagaa cgtcaggcca agcgaggcgg tatgtgcgcc
     2581 aagcgcataa tcgggcgtga tggctgtcg
//
LOCUS       DATMGX010000217         2418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_463354, whole genome shotgun sequence.
ACCESSION   DATMGX010000217 DATMGX010000000
VERSION     DATMGX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463354"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /gene="tkt"
                     /locus_tag="VNR65_03550"
     CDS             <1..1878
                     /gene="tkt"
                     /locus_tag="VNR65_03550"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWJ17783.1"
                     /translation="DRFVLSAGHASMLLYSTLHLAGVREVDHKTGEATKDLAVSLDEI
                     KRFRQLGSKTPGHPESHITTGVETTTGPLGQGVANSVGMAIAGKWLAANYNRPGLDLF
                     NFRVYSVCGDGDLMEGVAGEAASLAGHLQLSNLCWIYDNNRITLDGPASWSFGEDMMT
                     RFVGYGWNVTRVADANDLMMLARGYEVALKTNDKPTLIVVDSHIGYGSPHKQDSYEAH
                     GEPLGEAEIKLVKNNYGWPEDAKFLVPDGVYDHFRSGVGVRGGNAREKWNKLFADYSK
                     QFPDLASQLNKIKRRELPDGWDKNLPLFPADPKGMATRESSGKVLNALAQNIPWLIGG
                     SADLAKSNKTNLVFEGAGDFFPEQYKGRNIHFGVREHAMGAAVNGIVLTGPRAFSATF
                     FNFSDYMRASMRLGALMEINPIYIFTHDSIGVGEDGPTHQPVEQLASWRAMPNMVVLR
                     PGDANEVTEAYKVVAKLSHRPATMVFSRQAMPTLDRTKFAPASGVAKGGYVLLDAEGG
                     KPDVILMATGTELQIIVTASDLLKAQGIKARLVSLPSFELFDEQDPAYKESVLPSSIT
                     ARVSIEMSSTFGWDRYVGPKGKIIGMHSFGASAPLKDLLVKFGFTPEHVVKAAKEVLG
                     R"
     gene            1886..2341
                     /gene="rpiB"
                     /locus_tag="VNR65_03555"
     CDS             1886..2341
                     /gene="rpiB"
                     /locus_tag="VNR65_03555"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HWJ17784.1"
                     /translation="MIVVIGSDHAGFQLKTAMDSFLKSLGHTVLDIGAYNDQPSDYPD
                     FAELVGKAILDGRAERGILICGSGVGVSVAANKIIGIRAAVCHDSYSAHQGVEHDNMN
                     VLVLGSRIIGEKLAEDLVRNYLGASFTNEERHVRRLAKVHAIELRMLKN"
BASE COUNT          529 a          811 c          596 g          482 t
ORIGIN      
        1 gaccgtttcg ttctctctgc cggtcacgcc tccatgcttc tctattccac attgcacctt
       61 gccggtgtgc gcgaggtgga ccacaagacc ggtgaagcaa cgaaagatct cgccgtctct
      121 ctcgacgaaa tcaaacgctt ccgccaactc ggcagcaaga ctccgggcca ccccgaatcg
      181 cacatcacca ccggcgtgga aaccaccacc ggccctctcg gccagggtgt cgccaacagc
      241 gtcggcatgg ccattgccgg aaaatggctg gccgcgaatt acaacaggcc cggcttggac
      301 ctgttcaatt tccgcgtcta ctccgtctgc ggcgatggtg acttgatgga aggcgtagcg
      361 ggcgaggccg cgtctctcgc cggccatctg cagctctcca atctttgctg gatctacgac
      421 aacaatcgca tcaccctcga tggccccgcc agttggtcct tcggcgaaga tatgatgacc
      481 cgcttcgtcg gctacggctg gaacgtcacc cgcgtcgccg atgccaacga cttgatgatg
      541 ctcgcgcgtg gctacgaagt agccctcaaa accaacgaca agcccacgct catcgtcgtc
      601 gacagtcaca tcggttacgg ctctccccac aagcaagatt cctacgaagc ccacggcgag
      661 cccctcggcg aagccgaaat caagctggtc aagaataact acggctggcc cgaagacgcc
      721 aagttcctcg tccccgacgg tgtctacgat cacttccgct ccggcgtcgg tgtccgcggg
      781 ggcaatgcac gcgaaaaatg gaataagctc ttcgccgatt actccaaaca gttcccagat
      841 ctcgcttctc aactcaacaa aatcaagcgc cgcgaactgc ctgatggctg ggacaagaat
      901 ctccccctct ttcccgctga tcccaagggc atggccacgc gcgaaagttc cggcaaagtc
      961 ctcaacgcct tggcgcaaaa tattccctgg ctcatcggcg gctccgccga cctcgcgaag
     1021 tccaacaaga ccaatctcgt tttcgaaggc gcaggcgatt tctttcccga gcaatacaag
     1081 ggacgcaaca tccacttcgg cgtccgcgaa cacgccatgg gcgcagccgt caacggcatc
     1141 gtgctcaccg gcccgcgcgc cttttcagcc accttcttca acttcagcga ttacatgcgc
     1201 gccagcatgc gtctcggtgc tttgatggaa atcaatccca tctacatctt cacccatgac
     1261 tctatcggcg ttggcgaaga cgggcccacg catcaacccg tcgagcaact cgcttcctgg
     1321 cgcgccatgc ccaacatggt tgtactgcgc ccaggcgatg ccaacgaagt caccgaagcc
     1381 tacaaagtcg tcgctaaact tagccatcgc cccgccacca tggttttcag ccgtcaggcc
     1441 atgcctaccc tcgatcgcac aaaatttgcg cctgcctctg gcgtcgccaa gggcggctac
     1501 gtccttctcg atgccgaagg cggcaagccc gacgtaatcc tgatggccac cggcacggaa
     1561 ctccagatca tcgtcaccgc cagtgatttg ctgaaagccc agggcatcaa agcgcgtctc
     1621 gtcagcctgc cctccttcga actcttcgat gagcaagacc ccgcatataa agagagcgtc
     1681 ctcccctcca gcatcaccgc ccgcgtctcc atcgaaatgt ccagcacctt cggctgggac
     1741 cgctacgttg gccccaaggg caaaatcatc ggcatgcaca gtttcggagc atcggcaccg
     1801 ttgaaagatc tcctcgtcaa attcggcttc actcccgagc acgttgtgaa agccgcaaaa
     1861 gaagtcctcg gcaggtgaaa ctcttatgat cgttgttatc ggctccgacc acgccggctt
     1921 tcaactgaaa accgccatgg actcgttcct caaatccttg ggccacaccg tgctcgatat
     1981 tggcgcctac aacgatcaac cttccgacta tcccgatttc gccgaacttg tcggcaaggc
     2041 cattctcgac ggacgcgccg aacgtggcat tctcatctgc ggcagcggcg tcggcgtcag
     2101 cgtggccgcc aacaaaatca tcggtatccg cgccgccgtt tgtcacgaca gctattccgc
     2161 tcaccagggc gtcgagcacg acaatatgaa cgtgcttgtc ttgggctcgc gcatcattgg
     2221 cgaaaaactg gccgaagatc tcgtcaggaa ttatctcggc gcgagcttca ccaatgaaga
     2281 gcgccacgtt cgtcgcctag ccaaggtcca tgcaatcgaa ctgcgcatgt taaaaaacta
     2341 atatctggag ttttgccgcc gctcactggc gtacaatctt tctttaggcc tagtcagtaa
     2401 aggagaagtc tgatgacc
//
LOCUS       DATMGX010000218         5471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_430643, whole genome shotgun sequence.
ACCESSION   DATMGX010000218 DATMGX010000000
VERSION     DATMGX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430643"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VNR65_03560"
     CDS             <1..207
                     /locus_tag="VNR65_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17785.1"
                     /translation="GVKADGYFLLFQESGQHPVEDETCVELRVAAFYSLLVFSTGARE
                     AAQNPAALPMTISLQEHGPRNWTA"
     gene            complement(246..449)
                     /locus_tag="VNR65_03565"
     CDS             complement(246..449)
                     /locus_tag="VNR65_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17786.1"
                     /translation="MIERKFTIAEIILLAGTRIALGAGIGLLLSPWLNNDQRKAAGWA
                     LALVGGLTTIPLAMDVIGKKNSD"
     gene            complement(605..3193)
                     /locus_tag="VNR65_03570"
     CDS             complement(605..3193)
                     /locus_tag="VNR65_03570"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HWJ17787.1"
                     /translation="MPTKEQAISGDQDSSTKSLGRSRDQAGKPTKFIFDVALSTAIAA
                     NGERQAAVPAPKGPLTPDILDKMNRYWRAANYLCIGQIYLFENPLLREPLKAEQIKPR
                     LLGHWGTSPGQNLIYIHLNRLIKEHDATIIYVAGPGHGGPSLNASSYLEGTYTEVHPE
                     ITADENGLRLLFRKFSTPGGVPSHCGPHVPNSMHEGGELGYSLVHAFGAAFDNPDLIV
                     ACVIGDGESETCPLEGSWKSVNFLNPVRDGAVLPILHLNGYKISGPTVQARTSDEDLR
                     ALYIGRGYKPYFVEGDDPEVVHQILAGTLDECYAEIRAIQQEARQSGFKKQPIWPMIV
                     LRTPKGWTCPKEVDGIPIEGTFRAHQVPLATVRENPEHLKILEAWMRSYKPQELFDKN
                     GKFIAELAELAPAGNRRMGGNPHVNGGRLLTALDLPDFTNYALEIPGPGQVVAEAPRK
                     LGDFFRDVFKLNPTNFRMFCPDETNSNRLNSVLEATKRCFMAETVSIDDELSPDGRVM
                     EVLSEHCCQGWLEGYLLTGRHGMWSSYEAFAQVVDSMLTQHAKWMEQCRDYPWRKPLA
                     SLNVLLTSHAWRNDHNGFSHQAPGFVDNALARRSETIRVYYPPDSNCLLSVFDHCLRS
                     RNYVNIVTCGKQPQLQWLNMDEALEHCSRGASIWKFATNDGGNEPDVVLACAGDVPTI
                     EICAASWLLQKYVPGIKVRVVNVVDLSVLMMPDDHPHGMDNMSFDALFTKDAPVIFAF
                     HNNRWLIHTMVHGRSNESRFHVRGYMDQGTTTTPFDMVVLNKMSRFHLAIDALKYASR
                     LRSQVSDVIDMFDRKLYEHHDYIRQHLEDMPEIRNWGWTTDFNEPSAPPPLAKGHPRG
                     SVFSDS"
     gene            complement(3370..4239)
                     /locus_tag="VNR65_03575"
     CDS             complement(3370..4239)
                     /locus_tag="VNR65_03575"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HWJ17788.1"
                     /translation="MKIDSKDFRVGSGEKVTLSEWPTTIKPICKSKKQYQKLLEEHVE
                     ALSSLQRLHYASNRYALLLIFQGMDAAGKDGAIRHVMSGVNPQGCEVFSFKQPSAEEL
                     EHDFLWRTTCRLPERGRIGIFNRSYYEEVLVVRVHPEILRNQGLSEELRDEKTIWEER
                     YRSMVDLEGHLYRNGTRTVKIFLHLSLEEQRKRFLERLDEPDKNWKFSLSDIHERKYW
                     NDYMKAYEACLSATSTHHAPWYVVPADDKENARLIVSRIVLDALKGLKMAYPKPTEKR
                     RLELQSIRKQLAK"
     gene            complement(4543..5040)
                     /locus_tag="VNR65_03580"
     CDS             complement(4543..5040)
                     /locus_tag="VNR65_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HWJ17789.1"
                     /translation="MKINSKDFRVQPMVIPAEEDAGTVGTGRRLPILSRTQIQEKHTM
                     QVQINTGHNIEGHEALSAHVSDVVKNALVRLSDHITRVEVHLSDENGDKSGPNDKRCV
                     MEARLEGRQPVAVTHQAATLDQAVEGAAHKLTRLIESTLGRLHDQASRRTDPLPPEPK
                     AANPS"
     gene            complement(5083..>5471)
                     /locus_tag="VNR65_03585"
     CDS             complement(5083..>5471)
                     /locus_tag="VNR65_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17790.1"
                     /translation="EPNPEAKAEPKPHPKVEVQADQLKPDAKAEPKPEAKAESKPETK
                     VEAQADQPKPDAKAEPKPEAKAEPKPETKAQPKPEADDKTPSDLTPQLVKRVHELYEE
                     LGREDVREVEEWEKAKREIPKDEVPK"
BASE COUNT         1048 a         1563 c         1491 g         1369 t
ORIGIN      
        1 ggggtgaagg ccgacggtta cttcttgctg ttccaggaaa gcggccagca ccctgtcgaa
       61 gacgagactt gtgttgagct aagagttgcc gctttctata gccttcttgt attttcgaca
      121 ggcgctcgcg aagccgcaca gaatccggcg gctttgccca tgaccatttc gctgcaagaa
      181 cacgggcctc ggaattggac agcttgacag gacccagacc gtaccggtgc aggcgaccag
      241 gcgccctaat ccgaattttt cttgcctatc acgtccattg ccagtggaat cgtagtaagg
      301 cctccgacca gagcgagggc ccatcctgcg gctttgcgtt gatcattgtt taaccaaggg
      361 gaaagcaaaa ggccgatccc ggcccccaag gcgatgcggg ttccggcgag aagtatgatc
      421 tcggcaatgg tgaatttccg ttctatcatt ttttcaacct actctctttc ccgcaacgga
      481 ctcggcggga atcagttacg ggccaattcc ctctttgtcc gtcgtccaaa tggtcccatc
      541 cagatgttca aaggagcccc ctgccctctc attcaactct ccgcttcatc agtctgtttc
      601 tcacttagct atcgctgaac acgctcccgc gaggatgtcc cttggccagt ggcggagggg
      661 cgctcggctc gttgaaatca gtcgtccatc cccagttcct gatctccggc atatcttcca
      721 ggtgctgtcg aatatagtcg tggtgctcgt atagctttcg gtcgaacatg tcgattacgt
      781 ccgaaacctg tgaccggagc cgtgaggcgt acttcagtgc gtcgatggca aggtgaaaac
      841 gactcatctt attcagcact accatgtcga acggcgtcgt tgttgtgccc tgatccatgt
      901 agccgcgtac atggaaacgg ctttcattgg agcgcccgtg aaccatggtg tgaatgagcc
      961 agcgattgtt gtgaaacgcg aagatgacag gtgcgtcttt ggtgaagagc gcgtcaaagg
     1021 acatgttgtc cattccatgc ggatgatcgt ccggcatcat cagaacgctc agatccacca
     1081 cattgacaac cctcaccttg atgccaggaa catacttttg cagaagccat gaggctgcgc
     1141 atatctcaat ggttggcacg tcacccgcgc aggccagcac aacgtccggc tcgttacctc
     1201 cgtcgttggt cgcgaacttc cagatcgatg cacctcgcga gcaatgttcg agagcctcgt
     1261 ccatgttcaa ccactgaagc tgaggctgct tgccgcaagt gacgatgttg acgtagttgc
     1321 ggcttcgcag gcaatggtcg aagacgctca ataggcaatt gctgtccgga ggatagtaca
     1381 cgcggatggt ttcactccgg cgcgccagtg cattgtctac aaatccgggt gcctgatggc
     1441 tgaagccgtt gtgatcgttt cgccaggcat gacttgtcag tagaacgttt aacgaggcga
     1501 gtggtttacg ccagggataa tcgcggcatt gttccatcca tttggcatgt tgcgtcagca
     1561 tcgaatccac gacttgcgcg aaagcttcgt aggaagacca catgccatgt cgcccagtca
     1621 gcaaataccc ttcgagccat ccctggcaac aatgctcgct caatacttcc ataacacggc
     1681 catcggggct gagttcatca tcgatcgaaa ccgtttccgc catgaagcag cgcttggtag
     1741 cctcgaggac ggagtttaag cgattggagt tcgtctcatc ggggcagaac atgcgaaagt
     1801 tcgttggatt cagcttgaaa acatctctga agaagtcccc gagctttcgg ggagcttcag
     1861 caaccacttg acctggtcct ggaatctcga gtgcataatt tgtaaagtct ggcagatcga
     1921 gcgcggtcag cagccgtccg ccattcacat gaggatttcc tcccatgcgg cggttacccg
     1981 ccggcgcgag ctccgccaat tccgcaatga atttgccatt cttatcgaag agctcctgcg
     2041 gcttgtagct tctcatccag gcttcaagga tcttgagatg ttcggggttt tcccggaccg
     2101 ttgcgagggg cacctggtgc gcgcggaagg tgccttcgat tggaattcca tccacttcct
     2161 tcggacaggt ccatcccttg ggtgtgcgca ggacgatcat gggccagatc ggctgtttct
     2221 tgaatccgct ctggcgcgcc tcctgctgaa tggctcgaat ctcggcatag cattcgtcca
     2281 gcgttccagc caggatttga tgcacgacct ccggatcgtc gccttcgaca aaatagggct
     2341 tgtacccacg ccctatatac agggccctca agtcttcgtc gctcgtccgt gcctgaacag
     2401 tcggtccgga gattttgtag ccgttgaggt gcagaatcgg aaggactgcg ccgtcccgga
     2461 ccggattgag gaagttgact gacttccagc ttccttccag cggacaagtc tcggattctc
     2521 cgtcaccgat aacgcaggca acaatcaaat ccggattgtc gaaagcggcg ccaaaggcgt
     2581 gaaccagcga gtagccaagt tcgccgccct cgtgcataga attaggaacg tgcggtccgc
     2641 aatggctggg caccccgccc ggcgtcgaga acttcctgaa gagcagccgc aggccgttct
     2701 catccgccgt gatctccggg tggacttcgg tataggtgcc ttccaaatag gagctggcat
     2761 tgagcgaagg accgccatgt ccgggaccgg caacgtaaat aatggttgca tcatgctcct
     2821 tgatgaggcg gttcagatga atatagatga ggttctgtcc cggtgacgtt ccccagtgcc
     2881 cgagcagcct tggtttaatc tgttccgctt tcagcggctc tcgcaacagg ggattttcaa
     2941 agagatatat ctggccgatg cacagataat tcgcagcgcg ccagtagcgg ttcatcttgt
     3001 cgaggatgtc cggcgtgagc ggacccttag gggccgggac tgctgcttga cgttcgccgt
     3061 ttgctgcgat tgcggtagac aaagccacgt cgaagataaa tttagtcggc ttgccggcct
     3121 gatcccggct ccgccccagt gattttgtgc tgctgtcctg atcaccgcta attgcttgct
     3181 ctttcgtggg catactgatt attcctctct acctctccac cgcagctgac aagcccggca
     3241 gaggcttacg aacttcgatg agttcgcatc tgcgtgcgtt gcggcttcag agttaggtcg
     3301 tccatatcgt gcaggcattt caagctcgcc ttggcagcgc tcgtcaaata agggaccata
     3361 attccatcgc tacttcgcta gctgcttgcg gatcgattgc aattccagcc ggcgcttctc
     3421 ggtgggcttc ggatatgcca tcttgagtcc cttgagcgca tcaaggacaa ttcgggagac
     3481 gatcaaccgt gcgttctctt tatcgtcggc gggaacgacg taccagggtg cgtgatgggt
     3541 gctggtcgcg ctcaggcaag cctcatacgc cttcatgtaa tcattccaat attttctctc
     3601 gtgaatgtcg gagaggctga atttccagtt cttgtcgggt tcatcaagac gctcgagaaa
     3661 ccgctttcgc tgctcttcca gcgaaaggtg gagaaatatt ttgacggtcc gggttccgtt
     3721 gcgatagaga tgtccctcca ggtccaccat ggagcgatac cgctcctccc agatggtctt
     3781 ttcgtcgcgc aattcttccg atagaccctg gttgcggagg atctcgggat gcacccgaac
     3841 gaccagcact tcctcgtaat aggaacgatt gaagatgccg atccgtccgc gttcgggaag
     3901 gcggcaggtc gtacgccaga gaaaatcatg ttccagctct tcagcgctcg gctgtttgaa
     3961 actgaaaacc tcgcagcctt gcggattgac cccggacatg acgtgccgga tggcgccgtc
     4021 cttgccggca gcgtccatcc cctgaaaaat cagcagcagc gcatagcggt tggaggcgta
     4081 gtgaagccgc tgcagcgagc tcaacgcttc gacgtgttct tccagaagtt tctgatactg
     4141 cttcttcgac ttgcaaatgg gctttatcgt cgtcggccac tcgctgagtg tgaccttctc
     4201 tccggacccc actcggaaat ccttggaatc aattttcatc ttcttccttt cgctgtcacg
     4261 cgctgcgcga gtggcgctta gcaatcctgg ggtgcacgag gatgctgatg cacctttgcc
     4321 cgtccggcct ctaagccttt cgaatctcgc gctctgcctc aacccagccc tcatgtgctt
     4381 ggccttcgca gcggcgttga gggccaccgt cggttgcact ttcacgtcaa acgaccattg
     4441 cccgtcgcga tgccaatgtc catcatttct tctatacgca cagtgcctcg aaggcagaga
     4501 tggcaaccaa cagttcgctg cgcagacagc atatgctgtc gctcatgacg ggtttgcggc
     4561 cttcggctca ggtggaagcg ggtcagtcct gcgactcgcc tgatcgtgca gccgcccgag
     4621 ggtactctcg attaatcttg ttaacttatg tgcagcgccc tcgacagcct ggtcaagggt
     4681 cgctgcctgg tgagtgaccg cgaccggctg ccgtccttca agacgggctt ccatcacaca
     4741 gcgcttgtcg ttcgggccac tcttatcgcc gttttcgtcg ctcaggtgaa cttccactcg
     4801 cgttatatgg tcgctgagtc ggaccagggc atttttcact acgtcactga cgtgagcgct
     4861 cagcgcctca tggccttcga tgttatggcc agtattaatt tgtacctgca tggtgtgctt
     4921 ctcctgtatt tgtgttcggc tcaatatcgg taagcggcgg ccagtcccga ctgtcccggc
     4981 atcctcttcg gccggaatga ccatgggctg cacacggaaa tcttttgaat tgattttcat
     5041 ctccctccct tcggccatgt cagccgggtt gttctcgtct gtttatttag ggacttcatc
     5101 tttaggaatc tcacgcttcg ccttctccca ttcttcaact tctcgaacgt cctcacgccc
     5161 tagctcctcg tagagttcgt ggactcgctt gacgagctgt ggcgtcagat cggacggtgt
     5221 cttgtcgtcg gcttccggct taggctgggc tttagtttca ggcttcggct cggctttggc
     5281 ttcaggcttc ggctccgcct tagcgtcagg tttcggctgg tcggcttgcg cctcaacttt
     5341 agtttcaggc ttcgactcgg ctttggcttc aggcttcggc tccgccttgg cgtcgggttt
     5401 cagctggtcg gcttggacct caactttggg gtgcggcttc ggttcagctt tggcttcggg
     5461 gttaggctcc g
//
LOCUS       DATMGX010000219         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_303969, whole genome shotgun sequence.
ACCESSION   DATMGX010000219 DATMGX010000000
VERSION     DATMGX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303969"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VNR65_03590"
     CDS             <1..448
                     /locus_tag="VNR65_03590"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ17791.1"
                     /translation="ARQGLVLGVDRSPEMLELASRRNRRQIVEGRVQLRLASFGELPW
                     TSDTIDSILAVNVAYFFSPDAREIREARRVLKPGGQMAVYVTDKSTMSHWKFSGPDTH
                     ALYGEDDMRDLLARGGFNTADIAIRKVQLGFGIRGLLALGQKPPAS"
     gene            complement(539..>2455)
                     /locus_tag="VNR65_03595"
     CDS             complement(539..>2455)
                     /locus_tag="VNR65_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ17792.1"
                     /translation="LVSFDEITERNARAEALAAAKEEAERANLVKSRFLATVSHDLRQ
                     PLQTMTLVQGMLSEMISDPGAMTLIERLDRTISGMSNLLDKILNINQLEAGVVKPRHC
                     DFPINDLFDQLKDEFQIHAANDGLVLRVVPCHLMARTDPRLLEQILRNLLSNALKYTS
                     HGKVLLGCRRRGDSLSVEVWDTGTGIPETELGAIFKEYHQLRNHAAKGGKVLGLGLGL
                     AIVQHLADLLHTPIHVRSRLGRGSVFAVEVPVAQLSPAGAASLEHLAPSHRDRIPRAH
                     DSRSILIVEDDDEVRDALDLLLDRHGYSTLTARDGPQALALASDGSRDVDLIVADYNL
                     PGPNGLEVIARIEEASGRKVPAILLTGDISASTLLEIAAKGHVHLHKPANPRTLIRNI
                     NAILDNDGRTTSSSMALVADDARKVYGHASNAILGKDNEKASAPTVFVVDDAQDIREA
                     LRDMLQQHGYSTEIFADASAFLKDHSLGRAGCLVADVRMPGISGLELIERLGAMQSSL
                     PVIILTGYGDVAMAVKAMKAGAFDFLEKPVQMDELLRCIERALKFAGEHHDLSADRNT
                     AASKIESLTARQREILELVLAGTSSKNIAADLRISQRTVDNHRAAIMRKLGAKSLSAM
                     IRTALAATSSKSNP"
BASE COUNT          510 a          689 c          735 g          521 t
ORIGIN      
        1 ggcgcgccag ggtcttgtgc tgggggtcga tcggtcgccc gaaatgctgg agctggcttc
       61 gcgccgcaat cgccgtcaaa tcgttgaggg aagggtgcaa ctacgcctcg ctagcttcgg
      121 tgagctgcct tggacttcag acaccatcga cagcatcctc gcggtgaatg tcgcctattt
      181 tttctcgccc gatgcgcgcg aaatccgcga ggcgcgccgc gtgctcaaac ccggcgggca
      241 gatggcagtt tacgtcaccg acaagtccac catgtcccat tggaagtttt ccggccccga
      301 tacgcacgcc ctttatggcg aggacgacat gcgggatctt ctcgcgcggg gtggattcaa
      361 caccgctgac atagcgatca gaaaagtgca actcggcttc ggtatcaggg gactgctcgc
      421 tttaggtcaa aaaccaccgg cttcgtaaga cgagaacagg caaggccttc gcacggcagg
      481 ctcctgatca gcgggcgaac ctgctgctac cctgcaacac gcagcgcctt gaataagctc
      541 atggattgct tttggacgag gtggcagcga gcgcggtgcg gatcatggct gacagggact
      601 ttgccccaag tttccgcata atggcggcgc gatgattgtc gacggtgcgc tggctgatcc
      661 gcaagtctgc ggcaatattt ttgcttgaag tgccggccag cacgagctca agaatttcgc
      721 gctggcgagc cgtcaggctc tcgatcttgg aagcggcggt gttgcggtcg gccgacagat
      781 catgatgctc gcctgcgaac ttcagggcac gctcgatgca acgaagcaac tcgtccatct
      841 ggacaggttt ttcaagaaaa tcgaatgcgc ccgccttcat cgccttcacg gccatggcga
      901 catcgccata gccggtcaat atgatgacgg ggagcgatga ttgcatggcg cccaggcgtt
      961 cgatcagctc aagcccgctt attccgggca tgcgaacatc ggcaaccaga caaccggccc
     1021 ggccaagtga atgatccttc aagaaggcag aagcgtcggc aaaaatttcg gtgctatagc
     1081 catgctgctg cagcatatcc cgtagcgcct cgcgaatgtc ttgggcatcg tccacgacga
     1141 acactgtggg agctgaagct ttctcattgt cttttccgag tattgcattc gaagcatgac
     1201 catagacttt tctggcatca tccgcgacaa gcgccattga cgaagaagtc gtgcggccgt
     1261 cgttgtcgag tatggcattg atattgcgga tcagggtccg cgggttggct ggcttatgga
     1321 ggtggacatg gcctttggct gcaatctcaa gcagcgttga tgccgatatg tctccggtca
     1381 gaaggatagc cggcactttc cgtcccgagg cttcctcgat ccgggcgatg acctcaagcc
     1441 cgtttggacc tggaagatta tagtcggcga caatcaaatc cacgtccctg gagccatcgg
     1501 aggctagagc gagggcttga ggaccatcgc gggcggtcag cgtggagtag ccgtgtctat
     1561 ctaaaagtaa atcgagagca tcgcggacct cgtcgtcgtc ttcgacaatg agaatactgc
     1621 gactgtcatg agcacgaggg atacgatccc tgtgcgaagg ggccagatgt tcgagtgacg
     1681 cagctccagc cggagacagt tgcgccaccg gcacctccac cgcaaacacc gagcctcgac
     1741 ccaagcgcga ccggacgtgg atcggcgtgt ggagaagatc ggcaaggtgc tgcactattg
     1801 cgagaccgag accgaggcca agcaccttgc cgcccttggc tgcgtgatta cggagttgat
     1861 gatactcctt gaagatcgca ccgagttcgg tttcgggtat ccccgtcccg gtgtcccaga
     1921 cttcaacgct caagctgtcc ccgcgccgcc ggcagcccag taggactttg ccgtggcttg
     1981 tatatttcaa agcgttggac agcaggttgc gcaggatttg ttctaagagg cgcggatcgg
     2041 tgcgcgccat aaggtggcag ggtacaacgc gcaaaaccaa accgtcattg gctgcgtgaa
     2101 tctggaactc gtccttcaat tgatcgaaaa gatcgttgat agggaagtcg cagtgccttg
     2161 gcttcacgac gccagcctca agctggttga tgttgagaat cttgtccaag aggttcgaca
     2221 ttccgctgat cgtgcggtcg agcctttcga tgagcgtcat ggcgccaggg tctgagatca
     2281 tctccgacag catgccctgc accagggtca tggtctgaag cggctggcgc aaatcgtggc
     2341 tcacggtggc aagaaagcgc gactttacca aattggcgcg ttccgcttcc tccttggctg
     2401 ccgccagcgc ctcggctctg gcgttccttt cagtaatttc gtcgaaggag acgag
//
LOCUS       DATMGX010000220         3172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_414376, whole genome shotgun sequence.
ACCESSION   DATMGX010000220 DATMGX010000000
VERSION     DATMGX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414376"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="VNR65_03600"
     CDS             <1..1636
                     /locus_tag="VNR65_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HWJ17793.1"
                     /translation="NVAKLAPAWTFSTGVLRGHEGAPLVIGDVMYVHAPFPNTVFALD
                     LNNDGKILWKYEPKQDPTVIPVMCCDTVNRGLSYADGKVLLHQADTTLVALDAKTGAV
                     VWSVKNGDPSKGETGTSAPMVFKDKVLVGISGGEFGVRGSVAAYSLKDGKKLWRGYSM
                     GPDADTLIDPEKTTNLGKPVGADSGTNTWEGDQWKIGGGTTWGWYSFDPELNLMYYGS
                     GNPSTWNPTQRPGDNRWSMTIWARDVDTGKAKWVYQMTPHDEWDYDGVNEMILAEMDM
                     GGKKRKTLTHFDRNGFGYTLDRTNGELLVAEKFDPAVNWATKIDMDKASPNYGKPVRV
                     KKYSTEANGEDVNTKGVCPAALGSKDEQPAAYSPKTDLFYVPTNHVCMDYEPFRVSYT
                     AGQPYVGATLAMYPPPGENNMGNFIAWDGKTGKIAWSNKEQFSVWSGALATAGDVVFY
                     GTLEGYLKAVDAKTGKELYKFKTPSGIIGNVMTYEHGGRQYVAILSGVGGWAGIGLAA
                     GLTKPNEGLGAVGGYAALSNYTALGGTLTVFALPNG"
     gene            1733..2248
                     /locus_tag="VNR65_03605"
     CDS             1733..2248
                     /locus_tag="VNR65_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome, methanol metabolism-related"
                     /protein_id="HWJ17794.1"
                     /translation="MKANVRAFGLFIAACLLTSMAAAYGADDPTVASEKDGRYTDKEG
                     FPTYKVQPDGKVDWATFSGFRRYHAECHVCHGPDGEGSTYAPALKNSLKTIPYPEFLS
                     IVAAGRQNVSSSQENVMPSFGQNKNVVCYLDDIYVYLRARSTDAIPRGRPGKHEEKPD
                     ASKKAETECMG"
     gene            2363..>3172
                     /locus_tag="VNR65_03610"
     CDS             2363..>3172
                     /locus_tag="VNR65_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWJ17795.1"
                     /translation="MSITSVSSKIAAASLFTIGLAVASWSVAYAEDSEEPSSSLELID
                     PHVFRVCADPRNLPFSNEAGEGFENKLAELFAEKLDKKVAYAWYPGATGFVRNTLNAH
                     KCDVIMGFPQGSDIAQVTNPYYRTAYTLIFKPGNGLDGVDSLDDPILKTKRIGIVAGT
                     PPATYMARKGLLAKAKSYPLTIDTRFDSTSHAMIDDLGSGEIDAGILWGPIAGYYAKN
                     ANPKLSIAPLIKEQGGPRLAFRIGMGVRSSDQEFKRLLNRMIPENQREIDAI"
BASE COUNT          697 a          921 c          933 g          621 t
ORIGIN      
        1 gaacgttgcc aagctggctc ccgcctggac attctccaca ggcgtgcttc gcggacatga
       61 gggggctccc ctcgttattg gcgacgtcat gtacgttcat gcaccgttcc cgaatacggt
      121 attcgctctc gatctgaaca acgacggcaa gatcctctgg aagtacgagc cgaagcagga
      181 tccgaccgtc attcccgtga tgtgctgcga taccgtcaac cgcggccttt cctatgccga
      241 tggcaaggtc ttgctccacc aggcggatac gacgcttgtt gccttggacg ctaaaaccgg
      301 cgctgtcgtc tggtcggtca agaacggcga tcccagcaaa ggcgagaccg gcacctcggc
      361 gccgatggtc ttcaaggaca aggtcctggt cggcatctca ggcggtgagt tcggcgttcg
      421 cggttctgtg gcggcctaca gcctcaagga tggcaaaaag ctctggcgcg gctattccat
      481 ggggccggac gccgatacgc tgattgatcc ggaaaagaca accaatctcg gcaagcccgt
      541 cggcgcagac tcaggtacga acacctggga aggcgatcaa tggaagatcg gcggcggcac
      601 aacctggggc tggtattcct tcgatcccga actgaacctc atgtattatg gctcgggcaa
      661 tccctcgacc tggaacccga cccagcgtcc tggcgacaac cgctggtcca tgacgatctg
      721 ggcgcgcgac gtcgataccg gcaaggccaa gtgggtctat cagatgacgc cccatgacga
      781 gtgggactat gacggcgtca acgagatgat cctcgccgag atggatatgg gcggcaaaaa
      841 gcgtaagacg cttacccact tcgaccggaa tggcttcgga tacacgctcg atcgcaccaa
      901 tggcgaactg cttgtcgccg agaagttcga tccggccgtg aactgggcca ccaagatcga
      961 catggataag gcgtcgccga actacggcaa gcctgtccgc gtcaagaagt actcgaccga
     1021 agccaatggc gaagacgtca acacgaaggg cgtctgtccg gccgctctcg gctcgaagga
     1081 cgagcagcct gcggcgtact caccgaagac ggacctgttc tatgtgccga cgaaccacgt
     1141 atgcatggac tatgagccgt tccgcgtgag ctataccgca ggtcagcctt acgttggcgc
     1201 cacgctcgcc atgtatccgc ctccgggtga gaacaacatg ggtaacttca tcgcctggga
     1261 cggcaagacg ggcaagatcg cctggtcgaa caaggagcag ttctccgtgt ggtcgggcgc
     1321 tctggcaacc gctggcgacg tggtcttcta cggcacgctg gagggttacc tgaaggcggt
     1381 cgacgcgaag accggcaagg aactgtacaa gttcaagacg ccgtcaggca tcatcggcaa
     1441 cgtcatgact tatgagcatg gcggcaggca atacgtcgcc attctgtcgg gcgtcggcgg
     1501 ctgggccggc atcggtcttg cggctggtct gaccaagccg aatgaaggtc tcggcgcggt
     1561 gggcggctat gcggcgctct cgaactacac cgctctcggc ggcactttga cagtgtttgc
     1621 cttgccaaac ggctgatctc gaaacatatg atagtccagc gcgctgaaaa agcgcgctgg
     1681 aactttcttc gttccgaccg ttaccggcta cgccaagaat aataggggcc acgtgaaagc
     1741 taatgtcaga gccttcgggc tctttatcgc tgcctgcctg ctcacaagca tggctgctgc
     1801 ctacggcgcg gatgatccaa cggtcgcctc cgaaaaggat ggacggtata ccgataagga
     1861 gggcttcccc acatacaagg tgcagccgga tggaaaggtg gattgggcga cgttttccgg
     1921 ctttcgtcgc tatcacgccg aatgccacgt ttgccatggc ccggacggtg aaggctcgac
     1981 ctatgctccc gctcttaaga attctctgaa aacgatccct tatcccgaat ttctgagcat
     2041 cgtggctgct ggccgccaga atgtcagctc gtcgcaagag aacgtcatgc cctccttcgg
     2101 gcagaacaag aacgtcgtct gttacctcga cgatatttac gtctatctgc gcgcacgatc
     2161 gactgacgca attccacggg gacggccggg caagcacgag gaaaagccgg acgcgtccaa
     2221 gaaggccgag accgagtgta tgggatgatc cctagagcag ccaagtggac gcggattagc
     2281 tgctctaaga cttgagaatc gatcaacgtt tccgctttta atcgattcca attaaaggcg
     2341 ggttgatcta gaaagcgtaa gcatgagcat cacgtcagta tcgagtaaaa tcgccgccgc
     2401 ttcgctgttt acgatcggtc ttgccgtggc aagctggagc gtggcgtatg ccgaagactc
     2461 tgaggagcca tcctcttcgc ttgagcttat cgacccgcat gtctttcgcg tttgcgccga
     2521 tccgaggaac ctgcctttct cgaacgaggc tggggaaggg ttcgaaaaca agctcgcgga
     2581 actttttgct gaaaaactgg ataaaaaggt tgcctatgcc tggtatccgg gcgcgacggg
     2641 ctttgtacgc aacacgctga acgcgcataa atgcgacgtc atcatgggct tccctcaggg
     2701 cagcgacatt gcccaggtca cgaatcctta ttaccgcacg gcctatacgc tcatattcaa
     2761 gccgggcaac ggtctcgatg gcgtcgactc gctggacgat ccgatcctga agaccaagcg
     2821 gatcggcatc gtggccggga cccctccggc gacttacatg gccaggaagg ggcttctggc
     2881 aaaggcgaaa tcctatccgc tgacgatcga cacacgcttc gactcgacct cccacgccat
     2941 gattgacgat ctcgggtccg gggagatcga tgccgggatt ttatggggac caatcgccgg
     3001 ttactacgcc aagaacgcaa acccgaaact gagcattgct cccttgatca aggagcaggg
     3061 cggcccgcgg ctcgcgttcc gcatcggcat gggggtgcgc tcaagcgacc aggaattcaa
     3121 acgcctcctc aaccgaatga tcccggagaa ccagcgcgag atcgatgcga tc
//
LOCUS       DATMGX010000221         2778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_325765, whole genome shotgun sequence.
ACCESSION   DATMGX010000221 DATMGX010000000
VERSION     DATMGX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325765"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            510..2273
                     /locus_tag="VNR65_03615"
     CDS             510..2273
                     /locus_tag="VNR65_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWJ17796.1"
                     /translation="MAGKAVRNRAGERSVIAPRRFRVADIAQHLRLISGIILFLFAAT
                     HFLNHASGLISLELMHEVQQWRWSVTRSWPGTIILVSALLTHVVLGLYKTATRATLRL
                     SAWELVQLGLGVTIPFLLLPHIVNTRIAHVFFGVEDTYLYELARLWPASAVLQSLLLI
                     IVWAHGCLGLHKWLTFKSWYRHAEPLLIVLAVAIPVLALCGFMISGRSISELTKSPEQ
                     FKTLKELTHWPSPAAQDALARYRLGVRVFFGAALALAVAAFVWRRLALTGARRIIITY
                     TGGLSVKTPVGPTLLEMSRLHRVPHASACGGRARCSTCRVRIETSEKKLPRPAFAEAF
                     TLASIGAEPGIRLACQIRPRSSLTVTKLVREANFSPGDIAPSDLDAAGTEKPLAIMMV
                     DLRDYTRMAQGRLPYDVVYVLNEFFAATGNAIVTHHGTIDKYLGDGFLAFFGRERGVQ
                     AGCRDALCAVRAIDLALDHVNAKLEAELGSPLRIAIGLHAGECIIGRIGFGRSREVTV
                     IGNAVNVASRLEAVAKAHGFQIVASKDVIDNVAWPGNEAQTIEVEVRGVVSKMQVIGI
                     ARGRDLPISILTQSPEEYGNA"
BASE COUNT          566 a          752 c          777 g          683 t
ORIGIN      
        1 tcttttgatt tatatcatac ggatgacgtg tcttgaaatc cagtttcggc ctcgggttga
       61 gggcgtaagg caaagattgc aggttcccgg ccgggattca gtggctgttt aatttctaac
      121 ttctctatag cctcatacag aaaagctttt taatttcaat aaactgcagc ctgggcagtc
      181 ttgcaaaaaa atctcctggc cgcgctgacg ggatttgtac ggtgtgcatt gggaggaagc
      241 ttctgcagcg atgtcacata attgagctaa acctctctcc ctcactcgat caacccgcct
      301 ttaatcggat cgattagagg cggaaaagtt gatcgattct aaagtgctgg gtagcttatc
      361 cgcttccaga tggacgcggg ctgttccatg gcaacagaag tttttggtgg gcttttctat
      421 ctagcccgag catcattcct gccccacacc cagcttactg gcctgtggct ttggcagaca
      481 gtcgtgctca tgctgcttgt agaggttcca tggcaggtaa agcagtaagg aaccgcgctg
      541 gcgagagaag tgtcatcgcg cctcggcggt tccgggtggc ggacattgcg cagcaccttc
      601 gcctcatatc agggatcatc ctttttttat tcgcggcaac ccatttcctg aaccacgcct
      661 ccggtcttat cagtcttgaa ctgatgcatg aggtgcagca gtggcgatgg tcggtaacgc
      721 ggtcctggcc cggcacaatt atcctggtgt cggcgctttt aacccatgtc gtgcttggcc
      781 tctacaaaac cgcaacgcgt gcaacgttgc gtctttccgc ctgggagctt gttcaactcg
      841 ggcttggggt tacgatccct tttctcttgc ttccacacat cgttaacacg cgtattgccc
      901 acgtcttctt tggcgtcgaa gacacctacc tctacgaact tgcgaggctt tggcccgcga
      961 gcgctgtcct gcaaagctta ctccttatta tcgtttgggc tcatggctgc cttggcctcc
     1021 acaaatggct gactttcaag agctggtacc gccatgctga gcctttgctg atcgttctgg
     1081 cggttgcaat tccggtgttg gctctttgcg gcttcatgat ttcgggacgc tcgatatccg
     1141 agctcacaaa aagccccgaa caattcaaga ccttgaaaga gctgacgcat tggccaagcc
     1201 cggctgcgca ggatgcgctg gctcggtacc ggcttggcgt ccgtgtgttc tttggcgccg
     1261 ccttagcgct ggcggtcgca gcctttgtct ggcggcgtct ggcattgacc ggggcacggc
     1321 gcatcataat aacctacacg ggcggcctct cggtaaaaac ccctgtcggg ccaacgcttc
     1381 tcgaaatgag caggctccat cgggtcccac atgcgtcggc gtgcggcggg cgagcgcgtt
     1441 gttcaacctg ccgtgtgcga attgaaacca gcgaaaagaa actgccgcgg cccgcgttcg
     1501 ccgaagcatt tacgctggcg agtatcgggg cggagccagg aattcgcctc gcgtgccaga
     1561 tccggccgcg cagctcgttg acggtgacca aactcgtgcg agaggccaat ttttcgcctg
     1621 gcgatatcgc gccaagcgat ctcgacgccg ctggaacaga gaaacccctt gccattatga
     1681 tggtcgatct tcgcgattac acgcgaatgg ctcaggggcg cctgccttac gatgttgtct
     1741 atgttctgaa tgagtttttt gctgccactg gaaacgcgat cgttacccat cacggcacaa
     1801 tcgacaaata tctgggcgat ggttttcttg cctttttcgg ccgggaacga ggtgtccagg
     1861 ctggttgccg cgatgcctta tgtgctgtcc gtgcaatcga tctcgcgctg gatcacgtga
     1921 acgcgaaact tgaagccgag cttgggagtc ctctgagaat tgccattggg cttcacgcgg
     1981 gcgaatgcat catagggcgc atcggctttg ggcggtcccg tgaagtcaca gtgatcggga
     2041 atgcggtcaa tgtcgcgagc cgcctcgaag ccgtggctaa agcgcatggg tttcagattg
     2101 tggcctcgaa ggacgtgatc gataatgtcg cctggcccgg caacgaagcg cagacaatcg
     2161 aagttgaggt gcgcggcgtc gtttcgaaga tgcaggtcat cgggatagcg agaggccggg
     2221 atcttccgat aagcattttg acgcaatcgc cggaagaata cggcaatgct tgagatgagg
     2281 cgggcgacga gggcgacagg gcgctcgcaa gagccctccc tgtctgacat agatccggtc
     2341 gcggcaagcg caagttgatc gatggctgtc tcgtcaggac caggcttccg cgtcccatca
     2401 tggcgcattg aatcagacgc aggtgtccga atgcttgttt gtatgcatcg cgcaattatc
     2461 ggcatccgca tgtcaacttt tccctgacgc tttcgtctgc atttcttctt ctgaaatata
     2521 ttcagcgaat tcgctttgcc ggcaggttaa cgaaagctcc agcccgctcg aacccttata
     2581 aatatgagga aaataccgaa cgggcgccat cgccgccgcc tgcgccgcgg ctgttccggc
     2641 tgagcgcaag cgttaattcg cgaagcgcaa acatatttaa cggacttttg ctcgtcccag
     2701 taaatgaaaa aattccggca ttaaattcta tttaatggga ggtcatgagc gaatgaaagc
     2761 tgtttgcttg caaccatc
//
LOCUS       DATMGX010000222         2492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_154681, whole genome shotgun sequence.
ACCESSION   DATMGX010000222 DATMGX010000000
VERSION     DATMGX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154681"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1035..1214
                     /locus_tag="VNR65_03620"
     CDS             1035..1214
                     /locus_tag="VNR65_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17797.1"
                     /translation="MAGVAETAGIKVWDPLKRFGHWAFVIAFAVAYVTGGEEGEASDL
                     HEWAGYIVGGTSPSA"
     gene            complement(1285..>2492)
                     /locus_tag="VNR65_03625"
     CDS             complement(1285..>2492)
                     /locus_tag="VNR65_03625"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HWJ17798.1"
                     /translation="GLRVLGVARASFVGTIWPDSQHDFRFEFVGLVGLADPLRSSVPA
                     AVAECRTAGIKVCMITGDYPATAKAIARQAGLEADDVVTGDDLKMMNDAELARRVHTA
                     TVFARIMPEQKLRIVHALKSNGEIVAMTGDGVNDAPSLKAAHIGIAMGGRGTDVAREA
                     SSIVLLDDDFGSIVKAIRLGRRIYDNLRKAMGFIFAVHVPIAGLALMPLIFGLPILFG
                     PMHIAFLEMVIDPVCSLVFEAETEEDDVMRRGPRSPDEPLFSRHLIGWSLLQGGLAFA
                     LVAAIFVIGYRNGMPETEVRALAFFSLIMTIVSLIFVNRSFSASLISALGRPNPALAW
                     VLVAVTALLGLTLLWPLARNLFRFGPLHWDDLALTLGAGALVLVVLEVAKPIWRTRLE
                     STPDEKSSR"
BASE COUNT          510 a          735 c          746 g          501 t
ORIGIN      
        1 cggccatcag cgaattgggc gtgttggcct ccacggcacg gctgcgcgtc atcggcgcca
       61 tgacgatgcg gttcttcagc tgaagcgagc acgcggctaa aggttcgaag agcatgatta
      121 tccctggttg gtgaacaaat atgggcctcg caggcgaccc ataaggcagg ttctctggta
      181 acgcctaacc tactgcgcga ccgtgcgcta tggatcatgg gcggaaaaag aattccacaa
      241 ttggccgcaa gagcttagat ctaagattga cggtcgcccc aacgtcttct ggcgtccgca
      301 gcatttccgc gggatttctg aaagttgatt tcactgcttc cgattggctg aaaggcgacc
      361 atcttcgtcg cgattcctta cgcgtccttg gccggttgga gccgtccgat ttctggcgag
      421 gcgtttgtcg cttgattcgt tcttgacttg cccttgcccg gtcggaccta accgattact
      481 agcaagccgc ctgtcgcgcg aaggattctt tacgcgtcct tggccggtcg ggccctccct
      541 attcctggcg aggcgtttgt cggttctctc gtccttgact tgtccctgtc ccgtgggacc
      601 aagtctattt ccggcgagcc ttctgtcgcg tgaacgatcc tttacacgtc cctggccgcg
      661 ttgtttcacc gcagagtggg aagcggccga gtttgtcgag gcgactggtc gaattttcat
      721 gggcaaagcg gtcctgccac catcctcttc ttattccccg cctagtttgc acaaacagac
      781 attgacggct agaatcggaa actacccaga ctgaaagaat tcttagaagt cagtgcgccc
      841 cctcataacg ggtggttcca ttctatcact cctaacgcta cgcttcgggt catccgaaac
      901 agaatgccgc cgcgggaaaa cagcggctct gcgcaaaatc aaggcgcgtt gcgcaagact
      961 ttccaaagct tcgagatagt cgaaagcggt catgcaaggg cgccgctcgt gcagacgcag
     1021 cgggagatga gaccatggct ggggttgccg agactgcagg gatcaaagtt tgggatccat
     1081 tgaagcgctt tgggcattgg gcgttcgtca tcgccttcgc cgtcgcctat gtcacgggcg
     1141 gagaagaggg cgaagcgagc gacctgcacg aatgggccgg ctatatcgtc ggaggcacgt
     1201 cgccttccgc gtagtatggg ggctcgtcgg ccctcgatac gcccggttca gcgatttcgt
     1261 gacgggaccg gtctcgtcgc ttcgctacct gctcgatttt tcatcgggcg ttgactctaa
     1321 cctggtgcgc cagatcggct ttgcgacctc gagaacaacg agcacaagcg cgccagctcc
     1381 gagcgtcagc gccagatcgt cccagtgcaa cgggccgaaa cggaagagat tacgggcaag
     1441 cggccacaaa agcgtcaggc cgagcagcgc agtgaccgca acgagcaccc aggccagcgc
     1501 cggattcggt cttccgagcg cggagattag cgaagcactg aaggagcggt tgacaaagat
     1561 caggctgaca atcgtcatga tcaacgagaa gaatgcgagt gcacgcactt ccgtttctgg
     1621 catgccgttc cggtaaccaa tgacgaagat ggcggcaacg agcgcaaatg cgagcccgcc
     1681 ctgcaggaga ctccacccga tcaggtgtct ggaaaacaac ggctcatcgg gtgatcgcgg
     1741 tcctcggcgc atgacatcgt cttcttccgt ctctgcttca aagacgagtg agcagacggg
     1801 gtcgatcacc atctccagaa acgcaatgtg cataggcccg aacaggatag gcagaccgaa
     1861 tatcagcggc atcagggcga ggcccgcaat tggaacatgg accgcgaaga tgaagcccat
     1921 cgccttgcgc aggttgtcgt agatacggcg accgagccgg atggccttga cgatcgatcc
     1981 gaaatcatcg tcgagcagaa cgatagagga tgcctcgcgc gccacgtccg ttccacgtcc
     2041 gcccatcgca atgccaatgt gcgctgcttt aagcgacggc gcgtcattga cgccgtcgcc
     2101 ggtcatggcg acgatttcgc cgtttgactt gagcgcatgg acgatccgga gcttctgctc
     2161 cggcatgatc cgcgcgaaca cggtcgctgt gtgcacccgc cgcgcaagct cggcatcgtt
     2221 catcatcttg aggtcgtcgc cggtcacgac atcgtcggcc tcgaggccgg cctgacgggc
     2281 gatggctttg gcggtcgcgg gatagtcgcc ggtgatcatg cacaccttga tacccgccgt
     2341 ccggcattcg gcaacggccg cagggacact ggagcgcagc ggatcggcaa ggccgacgag
     2401 accgacgaac tcgaacctaa agtcgtgctg cgagtccggc cagatcgttc ccacgaagga
     2461 ggcacgggct acgccgagca cgcgaagtcc tt
//
LOCUS       DATMGX010000223         2100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_201866, whole genome shotgun sequence.
ACCESSION   DATMGX010000223 DATMGX010000000
VERSION     DATMGX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201866"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..1712
                     /locus_tag="VNR65_03630"
     CDS             435..1712
                     /locus_tag="VNR65_03630"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HWJ17799.1"
                     /translation="MANQDTAGVSHRVERDLRLDLFRGMGLWMIFLDHIPDDVVAWLT
                     LKNYGFSDAAEFFVFISGYLAGFIYGPIIRGGQFIAATKRLLTRVWQMYVAHIMLFLL
                     FTAQIARTVRKFDNPMYTDEFNVHNFLEHPDILIGQALTLRYKPVDLDVLPLYISLVA
                     VSPLVLWFMVRRPNWTLLGSVLVYIGARVFDWNLASYPPGTHWYFNPFAWQLMFFFAA
                     WCGVGGVTKLQGLIQSRVAQAVAVAWIAFALLIVMTWHSPFLESLIPKWMIKVIYPID
                     KTDLDMLRFTHFLALALLVARYFPRKSEILSSKWLRPMILCGQHSLPLFCFGVFLSFG
                     AHWILVQHAQGGARALWMQLLVSVGGILIMIGLAWLLNRAQQVPSLFVEVAEFDDGEA
                     AVDPGRVLSDALLSQDDDDRNKTHRGTPSRQCG"
     gene            1639..>2100
                     /locus_tag="VNR65_03635"
     CDS             1639..>2100
                     /locus_tag="VNR65_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17800.1"
                     /translation="MPYCRKMMTIETKHTAGRLLDSADNTLPWNKATQWIRRFDVYDV
                     ATLVLITALIVIALSTFKDYAISNDEAVQHHYGELIIAYYASGFKDLSVFSFQNLYLY
                     GGLFDIVVVALSHHLSIDPYDLRHILCALIGIGGIGAAAATARLIAGPRAAL"
BASE COUNT          418 a          617 c          556 g          509 t
ORIGIN      
        1 ttcccaaggc cggccgcggc ggctataacg atgccgctac aaagctataa gggcttcgtg
       61 aagatggcgc aaagcgtggc acgcaaaaat gacccgtaac gcgaatgata ttttagatac
      121 aaaaccactg gttgcgcaaa caactctcgc cagttgagcg ttctcctttt tcaataaacc
      181 tgaattaatt catattaagg aatggccaag gtcaattccc atgtgccccg cacacccgca
      241 aagcctcagc gattggaagt ctatcgtcgg agctttgatg cacacaaatc cagtgggttt
      301 tccgtccccc agagcttctt gcaccaattt atcattgcat ccacagaggc aggcatgaat
      361 tatgaccttt ttcaaggctg gcggcttttc ggactagctg actaagtctg actttaacca
      421 aatggataag cccaatggcc aaccaagata cagcgggtgt ttcgcacaga gtcgagcgcg
      481 acctacggct cgatctattc cgcggtatgg gcctatggat gatcttcctc gatcacattc
      541 cggatgacgt cgttgcgtgg ctgaccctaa agaattacgg cttcagcgat gccgccgagt
      601 tcttcgtgtt catctccggc tacctcgccg gcttcatcta cggcccgatt atacgtggcg
      661 gtcagtttat cgctgccact aagcgactgt tgactcgcgt ctggcaaatg tacgttgccc
      721 acatcatgct gttcctgttg ttcacggcac agatcgcccg cacggtaagg aagttcgaca
      781 atccgatgta cacggatgaa ttcaatgtgc acaatttcct cgagcacccc gacatcctaa
      841 tcggacaggc gctcacgcta cgctacaagc cggtcgatct cgacgtgttg ccgctctata
      901 tttccctggt cgccgtttca ccgctggtcc tgtggtttat ggtgcgccgc ccgaactgga
      961 ccctgcttgg gtcggtcctc gtgtacatcg gggcgcgggt gttcgactgg aatctcgctt
     1021 cctatccgcc cgggacacac tggtacttca atccgttcgc ctggcaattg atgttctttt
     1081 tcgcggcatg gtgtggcgtt ggaggcgtca ccaagctgca gggactgatc caatcccgcg
     1141 tggcgcaggc ggttgcggtt gcgtggattg cgtttgcgct actgatcgtc atgacctggc
     1201 acagcccgtt cctggaatcg ctgattccga agtggatgat caaggtgatc tatccgatcg
     1261 acaagaccga cctcgatatg ctgcgtttta cacacttcct tgcgttggca ttgttggttg
     1321 cccgatattt cccgcgcaaa tcggagatac tcagctccaa atggctgcgt ccgatgatcc
     1381 tgtgcggtca gcattcgctg ccgctttttt gcttcggcgt attcctgtcc tttggcgcgc
     1441 attggatcct ggttcagcac gcccagggcg gcgcgcgcgc actctggatg caactgctcg
     1501 tcagcgtcgg cggcatactg atcatgattg ggctcgcatg gcttctgaat cgggcgcagc
     1561 aggtacccag cctgttcgtc gaggtagccg aattcgatga tggcgaggcg gcggtcgacc
     1621 ccgggagggt tctttccgat gccctactgt cgcaagatga tgacgatcga aacaaaacac
     1681 accgcgggac gccttctaga cagtgcggat aatacgctgc cctggaacaa ggcgacgcag
     1741 tggattaggc gtttcgacgt ctacgatgtc gccacgctcg tcctcatcac cgcgctcatc
     1801 gtgatcgcgc tgagcacgtt taaagactac gccatctcca acgatgaagc cgttcagcat
     1861 cactacggcg aactgatcat cgcctattat gccagcggct ttaaggacct cagcgtcttc
     1921 agcttccaga acctctacct ctatggcggc ctgttcgaca tcgtcgtcgt cgccctcagc
     1981 catcatcttt cgatcgatcc ctacgatctc aggcatatcc tgtgcgcgct gatcggcatc
     2041 ggcggcatcg gcgccgccgc tgcgacggcg cgactgatcg cgggtccacg cgcggccctg
//
LOCUS       DATMGX010000224         2409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_110156, whole genome shotgun sequence.
ACCESSION   DATMGX010000224 DATMGX010000000
VERSION     DATMGX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110156"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1655)
                     /locus_tag="VNR65_03640"
     CDS             complement(105..1655)
                     /locus_tag="VNR65_03640"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HWJ17801.1"
                     /translation="MPTSEQPILLEIPEQPEPKARPRHGELKLKYVNRQQTMLAHICV
                     EELIPTDHKARAIWELAERVDLSRFTEGLRTTIGCPGRPGLDAQMLVSLWVYAYSEGI
                     SSAREIERLLPWEPGMMWLSGLETICAHKLSDFRVDHKEALDELFAELLAMLEGLGLV
                     SLEQVAHDGTKIRAQAGMDSFRREKTLRERLAEAREVVKQMGDPQVEAPAKSRQQAAQ
                     ERAARERMNRLEAAAKEMMVLKAEEKEQEEKDKVRVSLTEPEARKMKHGDNAIAPSYN
                     AQITTESEHKIIVGAHATQCSSDANSLLPALEEVAQNLTKTPQQVIVDGGFTNRENIL
                     ECARQGIDLVGSLADPKEKSEAAMKANGIDRNFAPHYFHVLENGLRLECPAGCSLPQL
                     GKSVKHGNLYQQYRARGEDCLSCRYQRQCCPRKPEGGRTVSIRLEEHPDMAAFRQKMK
                     SEESKAIYRRRGEVAEFPNAWIKDKLGLRKFRVRGLIKAETELIWACLTYNIMQWVRL
                     VWRQRAMA"
     gene            1793..>2409
                     /locus_tag="VNR65_03645"
     CDS             1793..>2409
                     /locus_tag="VNR65_03645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWJ17802.1"
                     /translation="MIGRTISHYQVIEKLGAGGMGDIYKAQDARLHRMVAIKALTNAS
                     AGDTDRRRRFIQEAQAASALNHPNIITIYDIVSEDDSEYMVMELVNGKTLADLIPPGG
                     VGVAKTLQYSTQMADALRAAHAAGIVHRDLKPGNVMVTDSGLVKILDFGLAKMNVATA
                     LTEETQTLEAAPLTMEGSILGTVAYMSPEQAQGKKVDARSDIFSFG"
BASE COUNT          430 a          776 c          655 g          548 t
ORIGIN      
        1 tgctcaagat gatgctaagt gattgattgt gtggcgttaa tttccgtttt gcccgatgcg
       61 gaggcactac ggcgcttgcc cgttcccgag gttcctctcg gggcttaggc catcgctcgt
      121 tggcgccaca ccagccgcac ccattgcatg atgttgtacg tcagacacgc ccagatcaac
      181 tcggtctccg ccttgatcaa tccgcgtacc cggaattttc gcaaccccag tttgtccttg
      241 atccatgcgt tgggaaactc cgccacctcg cccctccgcc ggtagatcgc cttactctcc
      301 tcgctcttca tcttctgccg aaatgccgcc atgtccgggt gttcttccaa ccggatcgac
      361 accgtccgtc cgccttccgg ttttctcggg caacactgcc tctgatagcg gcaactcagg
      421 caatcttccc cgcgcgcccg atactgctgg tacagattgc catgtttcac actcttgcct
      481 aactggggca agctacatcc cgctggacac tccagcctca gtccgttttc caggacatga
      541 aagtagtgcg gcgcaaagtt tcgatcgatc ccattggcct tcatcgccgc ctcgcttttc
      601 tccttcggat cggccagcga tcccaccaga tcgatgcctt gcctggcgca ttccaggatg
      661 ttctcccgat tcgtgaagcc cccatccact atcacctgct gcggcgtctt cgtcagattc
      721 tgggccactt cctccagcgc cggcagcagg ctgttggcat cactgctgca ttgcgtggcg
      781 tgggccccga cgatgatctt gtgctccgac tcggtggtga tttgcgcgtt gtagctgggt
      841 gcgatggcgt tgtcgccgtg cttcattttg cgcgcttccg gttccgtcaa gctgacgcgc
      901 accttgtctt tttcctcctg ctctttttcc tcggccttca gcaccatcat ctctttggcg
      961 gccgcctcca aacgattcat ccgttcccgt gcggcccgct cctgggctgc ctgttggcgg
     1021 ctcttcgccg gggcttcgac ctgcggatct cccatctgct ttaccacttc ccgcgcttct
     1081 gccagccgct cacgcaacgt cttctcccgc cggaagctgt ccattcccgc ctgcgcccga
     1141 atcttagtcc cgtcatgcgc cacctgctcc agactcacca gccctaaccc ctccaacatg
     1201 gccagcaact ctgcgaacaa ctcatccaag gcctctttat ggtccacccg gaaatccgac
     1261 agcttgtgtg cgcagatcgt ctccagtccg ctgagccaca tcatgcccgg ctcccacggc
     1321 aacaaccgtt cgatctcgcg tgccgaactg atcccttcgc tgtaggcata tacccacaga
     1381 ctcaccaaca tttgcgcatc caaacccggt cgacccgggc atccgatagt ggttcgcaaa
     1441 ccctccgtga accggctcaa atccacccgt tccgccaact cccatatcgc gcgcgccttg
     1501 tgatcggtcg gaatcagttc ctcgacgcaa atatgcgcca gcatggtctg ttggcgattg
     1561 acgtacttca acttcaattc accgtgtcgg ggcctggcct ttggttcggg ttgctcgggg
     1621 atctccagca agatcggctg ctcgctagtc ggcatacttc tattatcgga aaagtacttc
     1681 tcaaatcaaa gtattttgcc aaaataattg aataatgctc accttctaac ggtcgcggct
     1741 cggattgagg cttggaacac ccaggggccg aagggtgtga caaactagtt tagtgattgg
     1801 tcggacgatt tcgcattatc aggttatcga gaagctgggc gccggcggca tgggcgatat
     1861 ttataaggcc caggacgcgc ggctgcatcg catggtggcc atcaaggcgc tgaccaatgc
     1921 gagcgcgggc gatacggacc ggcggcggcg gttcatccag gaggcgcagg cggcctccgc
     1981 actgaatcac cccaacatca tcaccatcta cgacatcgtg tcggaggacg actccgagta
     2041 catggtgatg gagctggtga atgggaagac gctggccgat ttgattccgc ccggcggcgt
     2101 gggcgtggcc aagacgctgc aatacagcac gcaaatggcg gacgccctgc gcgcggcgca
     2161 cgcggcgggg attgtgcacc gcgatctcaa gccgggcaac gtgatggtga cggattcggg
     2221 gctggtgaag atcctggatt tcggcctggc gaagatgaac gtggcgacgg cactcaccga
     2281 ggagacgcag acgctcgagg cggcgccgct gacgatggaa ggctcgattc tggggacggt
     2341 ggcgtacatg tcgccggagc aggcgcaggg caagaaggtg gacgcgcgga gcgacatttt
     2401 ctcgttcgg
//
LOCUS       DATMGX010000225         3470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_386917, whole genome shotgun sequence.
ACCESSION   DATMGX010000225 DATMGX010000000
VERSION     DATMGX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386917"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VNR65_03650"
     CDS             complement(<1..711)
                     /locus_tag="VNR65_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ17803.1"
                     /translation="MALAPKLALRNLFQDPLRLIATVVGIGFSMVLVTVQIGLYVSFQ
                     KMVTVMIDHADADLWIVPRGSKSFEDPSWLDEHERFRALSINGVLGAVPLVVGLGRWR
                     APSGATSPIFIVGADRREDGLQPWNLVDGNIESLSIPGAVAIDQSYFDRLETRRIGES
                     AEINDQTVKVLEITHGIRSFTTMPYVFVSPDLARTLTGTPPNKASYFIVHLTPGANAA
                     ETREELRARLVDDDVLTPD"
     gene            complement(951..1376)
                     /locus_tag="VNR65_03655"
     CDS             complement(951..1376)
                     /locus_tag="VNR65_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17804.1"
                     /translation="MKRIAFAALLLIATSSLGTITFAQKEKADVGKLEFEVHCAICHG
                     MNAKGNGPYVSSLKLSPPDLTLIAKNNGGVFPADRIIGVIDGRAQIASHGSRDMPIWG
                     DRYAITAAEHFAGSPYDQEAFIRGRVLILVDYLSRIQQK"
     gene            complement(1559..>3470)
                     /locus_tag="VNR65_03660"
     CDS             complement(1559..>3470)
                     /locus_tag="VNR65_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HWJ17805.1"
                     /translation="ERFSGRKHTNAYPRASLEALTEIMKDLGIGPERIVACLATYDYS
                     LLIAAGLRSVLEEFPASLHLAFQGNAPTFDGDQLKEGFKAPARLGQLFGLDCGVPIIG
                     MPHHDNHAWFSYLASPFARERQKTMIVVVDGSGDFASTTLYLGENGVVRQIQSNASMF
                     DSLGMFYGIISSTQGGWTALSSEGRYMGAAAYGDMDRSTNTFYRQLRNIFDLRADGDV
                     YLNRSLANWPRRMLGQPYTQDLTEILGPPIAPEEMWNPDAVLRVEDIHHRPNTQERLN
                     KAAATQMVFEDALVHIIDGLIRMTGSDRLVLTGGAALNAAANMRVLEHFDAGYYGRVL
                     KRSTRLHFWTPPVPNDAGVTVGAAYAFAAAAGVGLGPSLQHAFYCGRAPRMSDILVAL
                     EGAADVAWISVGDASHISGIEAIADLMAFITARDGIIAIFQGPAETGPRALGHRSILA
                     NPCNPRTRELLNERVKYREAIRPLAPMATLSAAKALFDLSEGGSDDEHNAYNYMVLTA
                     RAKPQARAQVPAVIHVDGTARLQIVREHTDAVTHAYLKALGRRIGVEVAVNTSFNVGG
                     PIAQTPVQALDTLRRAKGMDGVFMFSEEGQVIAAWTRKPGTRAAARIPEWLVEWQSET
                     NAAVELSGARSC"
BASE COUNT          726 a         1011 c         1021 g          712 t
ORIGIN      
        1 gtcgggcgtg aggacgtcgt cgtcgacgag ccgggcgcgc agctcctcgc gagtctcggc
       61 tgcattcgcc cccggggtca ggtggacgat gaaatacgac gccttgttgg gcggagtgcc
      121 tgtaagggtg cgcgccagat ctggagagac aaagacataa ggcatcgtcg tgaaggagcg
      181 gatgccgtgg gtaatttcca gcactttgac cgtttgatcg ttgatttcgg cgctttcacc
      241 gatccggcgg gtttcgagac gatcgaaata ggactggtcg atcgcaacgg ctccggggat
      301 tgagagcgat tcaatattgc cgtcgacgag attccagggc tggagtccat cctctcgccg
      361 atcagccccg acaataaaga tcggagaagt cgctccagat ggtgcgcgcc agcggccgag
      421 gccgaccacc aacggaaccg cccccagaac tccattgatc gaaagggcgc gaaaccgctc
      481 gtgttcgtcc agccatgacg ggtcttcgaa acttttcgat ccccggggca cgatccagag
      541 atcggcgtcg gcatgatcga tcatcaccgt caccattttc tggaaactca catacaggcc
      601 aatctgaacg gtcacgagaa ccatcgagaa gccgatgccc acgacggtgg caatcaatcg
      661 caacggatcc tgaaacaggt tgcgcaacgc aagttttgga gcaagcgcca taattgtgtc
      721 cgagggaacc tatctgtgcc gaaaggtata ttggtttcat gagcgcacgc ggcgccacgg
      781 cgacagcagt acagttggaa aacgcatgcc tcgagcggcg acaccttgat ttgcctcaat
      841 gtgccgcttt tttgaacgca gacgtgtgta tcgcgtatgc gcgtgctaat aagaactctg
      901 cacggttgcg cttgatttag gccctgttca aacagccatc gtttctcggt ctatttctgc
      961 tgaatacggc tgagatagtc gaccaatata agcactcgtc cacgtatgaa ggcttcctga
     1021 tcataaggag agccggcaaa atgttccgcg gcagtgatcg cgtatcgatc gccccaaatc
     1081 ggcatgtcgc gagagccgtg gcttgcgatc tgtgcccggc catctatcac gccgatgatg
     1141 cggtcggctg gaaacacacc cccattgttc ttggcaatca atgtcaggtc aggcggcgaa
     1201 agcttgagac tggaaacgta tgggccatta cccttcgcat tcatcccgtg gcagatggcg
     1261 caatgcacct cgaattccag cttacccaca tcggcctttt ctttttgagc gaatgttatc
     1321 gtaccgagcg atgatgtcgc tatcagcagg agtgcagcga aagctattct tttcataggt
     1381 gcctccttgg ctcccgatta caaaccaatt gggcttcacc gacgcctcga caaaatctat
     1441 ttctcctgga ttcgacccag atactcgacg acggccaaaa tacggctccg tatgaccgga
     1501 tcgggatcgc aagcgatgtc aagatgagat tctaaatgag gccgggatag cgctcgtttc
     1561 aacacgagcg cgctccggat aactcgaccg cggcgtttgt ttcgctttgc cactccacga
     1621 gccattcagg gatccgcgcc gcggctcggg ttccaggttt cctggtccag gccgcgataa
     1681 cctgcccttc ctctgagaac atgaatacac cgtccatgcc cttcgcgcgg cgcagggtat
     1741 caagcgcctg gactggggtt tgcgcgatcg gtcccccgac gttgaaggat gtgttcactg
     1801 caacctcgac gccgattcgc cttccaagcg cctttaaata ggcgtgcgtt acggcatcgg
     1861 tatgctcgcg cacgatctgc agccgcgcag ttccatcaac gtgaatgacg gcgggaactt
     1921 gggcgcgcgc ttgaggcttg gctctcgcgg tcaggaccat gtagttatag gcattgtgtt
     1981 catcgtccga gccgccctcg gacaagtcga acaaagcctt tgccgccgaa agcgtcgcca
     2041 taggcgcgag cggccggatc gcttcgcgat atttgacgcg ctcgttaagc agttcgcgcg
     2101 tgcgcggatt gcaagggttg gccaagatcg atctgtgacc cagcgcccgc ggccctgtct
     2161 ctgccggtcc ctggaagatc gcaataatcc cgtctctcgc agtgatgaac gccatgaggt
     2221 cggcgatggc ctctattccg gatatgtgcg acgcatcgcc gacgctaatc caggccacat
     2281 cggcggcacc ctccagagcg acgaggatat cggacatgcg gggcgcgcga ccgcaataga
     2341 aagcgtgctg gagggacggc ccgagaccca ccccggcggc ggcggcgaat gcgtaggctg
     2401 ctcctaccgt taccccggcg tcgttcggca caggcggcgt ccaaaaatgg agccgcgtcg
     2461 atcgcttgag gacgcggcca tagtagcctg cgtcgaaatg ctccagcacg cgcatgttcg
     2521 cagctgcgtt cagcgccgcg cctccggtca gcaccagccg gtcgctgccg gtcatgcgga
     2581 tcagaccgtc aatgatgtgg acgagagcat cctcgaaaac catctgggtg gccgccgcct
     2641 tgttgagcct ttcctgcgta ttcggccggt gatggatgtc ttcgacacgc aggaccgcat
     2701 cggggttcca catctcctca ggcgcgatgg gcggccccag gatctcggtg agatcctggg
     2761 tatagggctg accaagcatg cgccggggcc aattcgcaag cgaccggttg agatacacgt
     2821 cgccgtctgc ccgaagatcg aagatgtttc gtagctgccg atagaaagtg ttggttgaac
     2881 ggtccatgtc gccataggcc gcggcgccca tgtagcggcc ttcgctgctg agcgctgtcc
     2941 agccgccctg cgtcgagctg atgatgccgt aaaacattcc gagcgagtcg aacatgctgg
     3001 cgttgctctg gatttgccga acgacaccgt tctcgccgag atagagcgtg gtcgaggcga
     3061 agtcgcccga accatcgaca actacgatca tcgtcttttg ccgctctctg gcgaaaggcg
     3121 aggccaaata tgagaaccat gcgtgattgt cgtggtgcgg catgccaatg attggcacgc
     3181 cgcaatcgag gccgaaaagc tgacccaacc gggctggggc tttaaatccc tccttcaatt
     3241 ggtcaccgtc aaaagtcggg gcgttaccct ggaacgccag gtgcaggctt gcgggaaatt
     3301 cctccagaac ggaccgaagc cccgcggcaa taagcagaga gtaatcgtag gtcgcgagac
     3361 aagcgacgat ccgctcaggg ccaatgccca aatccttcat gatttcagtc agcgcttcga
     3421 gcgatgcgcg aggataggcg ttggtatgct ttcggcccga gaagcgctct
//
LOCUS       DATMGX010000226         3107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_262014, whole genome shotgun sequence.
ACCESSION   DATMGX010000226 DATMGX010000000
VERSION     DATMGX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262014"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..895
                     /locus_tag="VNR65_03665"
     CDS             191..895
                     /locus_tag="VNR65_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ17806.1"
                     /translation="MTPASDKTRLLVVDDEPPIRRLLRTSLGAEGFEVLEAENAEKAL
                     SLIGSAKPEPEVVILDLGLPDLDGLDVIRRIRAKGSKLPIIVLSSRGDERGKVEALDL
                     GADDYVTKPFGIAELVARIRAALRHRVQEQGGEPLFKSGSLQVDLVRRVVTVGGEEVK
                     LSPKEYDILRLLVLHAGKVLTHRFLMQQVWDAETEVQYLRIYVRQLRQKIEVNPERPA
                     HIITETGVGYRLRVQN"
     gene            complement(1416..1844)
                     /locus_tag="VNR65_03670"
     CDS             complement(1416..1844)
                     /locus_tag="VNR65_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17807.1"
                     /translation="MFSANEPTDGVVLRMNTAQHQLLRDFRILVAEDDVILALDMESS
                     LRGAGAQVFGPVKTTADATALAWTAPLTCAVLDVNLGCELVFPAAHVLKDRNIQTIFC
                     TGYRDLRGLQKDWPDAKILSKPVHPQLLIRTVRSAHEHPS"
     gene            2693..>3107
                     /locus_tag="VNR65_03675"
     CDS             2693..>3107
                     /locus_tag="VNR65_03675"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWJ17808.1"
                     /translation="MTYCGIAQIAAFTALIALLTKPLGYYMARVFAGERTMLRPVLGP
                     VEKALYRVAGVDPAKEQSWLDYALSFLAFNLFGILALYAVQRLQFVLPVNPQNMAGVS
                     PDLSLNTAISFTTNTSWQSYAGETTLSYFSQMMGIT"
BASE COUNT          673 a          859 c          877 g          698 t
ORIGIN      
        1 ccaccgcgtg aaacagggcg acagccagcg cgcgggacct ggtctcgggc tcgctatttg
       61 ccgtggattt gtgcagcccc tcggaggcaa gattgtcgcg gcgaacaggg ccgatcgttc
      121 cggcgcgatc ttgacgatca cctttcccta cctcgaaaag gaactggccg cgtgaagcag
      181 ccggcacacc atgaccccag cgagtgacaa gacgcggctt ttagtggtcg acgacgaacc
      241 gccgatccgg cgtctgctgc gcacgagtct cggggcagaa ggcttcgaag tgctggaggc
      301 cgagaacgcc gaaaaggcgc tcagcttgat tggatcggca aagccggagc cggaggtcgt
      361 gatcctcgat ttgggcttgc cggaccttga cggtctggat gtcatacgcc gcattcgcgc
      421 gaaagggtcc aaactcccca tcatcgtctt gtcgagccgc ggcgacgagc gcggcaaggt
      481 ggaggcgctc gaccttggcg cggacgatta cgtgacgaag ccctttggca tcgcggaact
      541 tgttgcgcgc atccgcgccg cgttgcgcca ccgcgttcag gaacagggcg gcgaacctct
      601 cttcaaaagc gggagcctgc aagtggatct tgttcgccgc gttgtaaccg tgggcggaga
      661 agaggtgaag ctttcgccca aggagtacga cattcttcgt ctcctggtcc tccacgctgg
      721 gaaggtgctg acccatcgct ttttgatgca gcaggtttgg gatgcggaaa ccgaggtgca
      781 atatctgcgc atctatgtcc gccagttgcg ccaaaaaatc gaagtcaatc cggagaggcc
      841 tgcccatatc attaccgaga ctggcgtcgg ctatcggctc cgggttcaaa attagacttg
      901 gattgatctt gcgttgccgc tgagtcgtga gataggaatc gacctctcga ttgaggtttg
      961 acgccaatct ccgacaccag gcgctcgaag gccagccaga agctgggtgc caaattccac
     1021 gtcaccttta gccgcacgcc tatcgagaac tatatcgacg agtattcgag cctattgaca
     1081 ccatcaaccc cggcttgctt ggctcgcgcg aaagctttca gcgccgttta tcttatcgag
     1141 cgcgaccgga agcctgccgc tcgacgggtt tcgcgcgcaa gctggccatt ttccaggaac
     1201 tcaccgacga gctacagcgt gcttccaaag tcgcggagct gactgggcgc atcgaaacgc
     1261 tcagggcgcg catgcaaagc ctggctgcac tattggctaa aggaccgcaa aatggatcgg
     1321 ggcgaggaaa ataagcgtca cagccacact gattgacgca gcgttttatt atagattgca
     1381 aaccatgccc gatttcccgt gggccgccgt cggcctcaag atggatgctc gtgtgcggag
     1441 cgaacggttc gtatcaagag ctgagggtga acgggcttgg aaagaatttt agcatcgggc
     1501 cagtcctttt gaaggccgcg cagatctcta tagcccgtac aaaaaatcgt ttgaatattg
     1561 cgatccttca aaacatgtgc agccgggaaa acaagctcac agccaagatt cacatcaagc
     1621 acagcgcagg taagcggagc tgtccacgcc aaagcggttg catccgccgt tgtctttacc
     1681 ggtccgaaga cttgcgctcc agcgccgcgc aacgaactct ccatgtcaag agcgaggatc
     1741 acatcatcct cagcaacgag aattcgaaag tctcgcaaaa gctggtgctg agccgtattc
     1801 attcttagta caactccatc ggtaggttca tttgcggaga acattagcca ctcttccaag
     1861 tgctgttttg cttcatgtca ataaagtacg atcatccagg caaaattttg tagcgctccg
     1921 caattggaat aaacacgcaa aaccgtgctg ttctttctat tgctaagccc taggcatctt
     1981 aagcttcgca ttgaacgaca aatgcatgcg gttctgagat atttgcggca gtaaaattgg
     2041 tcgcgcacct cgtcataggt tctggcaggc gtcgcctcga cgtccacgcc gtggtccgca
     2101 cctgcatggc ggctggccgt tgaggccagc gtcatggaag ctgatgctag ccgcaatcat
     2161 ggtgttgttt atgcgtaata tggacatcgc ccggccggga aactgtgatt catcacagta
     2221 gacgctcgga tgccgctgcg cgattaagcg ctttctatcc caacagtgtc taatgctcgc
     2281 ggagttacga gcggcccaga atgacgatcg ggggctttga cgagagtcaa cgacatacgc
     2341 ggcatcgtgc ccagaaagga cgactatcct gcggcacgtc atcgctaccg agactgccgc
     2401 cggtatgtct tttgtatata agtttcctac ggccaccagc attcgctcac ctcgtattta
     2461 tctgactttt ctgatttaat ggcacgggtg aagcgccaac gatgtcgcat cctatgttga
     2521 gagcataaca atcagcatcg gcagggcatt tcttgggctg ccggaagtgg cggggggact
     2581 ctttgaccga catcgccata gtgctgtttg tcgtcgccgg cattattgcg ctcggttttt
     2641 atgcccgcgc ctgccgctcc ttgatccgaa gcctggatgg cgaggggcag aaatgactta
     2701 ttgtggcata gcgcaaattg ctgcctttac ggctcttatc gcgcttctga cgaagccgct
     2761 tggctattat atggcccggg tttttgccgg cgaacggaca atgcttcggc cagtgcttgg
     2821 accagtcgaa aaggcgcttt accgagttgc tggcgtcgat ccagccaaag agcagagttg
     2881 gttagactac gccctttcgt ttctcgcctt caatttgttt ggaattctgg cgctctacgc
     2941 ggtgcagcgg ctgcagtttg tgctaccggt caatccgcag aacatggcgg gcgtttctcc
     3001 cgatctctct ttgaacacgg ccatcagttt caccaccaat acgagctggc agtcttacgc
     3061 gggcgaaacc acgctcagtt acttttcgca aatgatgggg ataacgg
//
LOCUS       DATMGX010000227         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_78223, whole genome shotgun sequence.
ACCESSION   DATMGX010000227 DATMGX010000000
VERSION     DATMGX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78223"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VNR65_03680"
     CDS             complement(<1..1273)
                     /locus_tag="VNR65_03680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HWJ17809.1"
                     /translation="MATIEGSARSAAAGVAGLLSRRRIIAKTGFNRWLVPPAALAIHL
                     CIGMAYGFSVFWLPLSKALGIKAPVACPDMGLGQALFTTTCDWRVADLGWMYTLFFVF
                     LGLSAAVFGGWVERVGPRKTGIVSAFCWCGGLCISALGVRYHQLWLMWLGSGIIGGIG
                     LGLGYISPVSTLIKWFPDKRGMATGMAIMGFGGGAMIGSPLAVILMQHFQTPADVGVW
                     QTFLALAAIYFVFMLGGAFGYRVPPDGWQPDGWTPPLKKKSLITVHHVHVRDAYKTTS
                     FWLIWLVLMLNVSAGIGVIGIASPMLQEIFGGALIGKPDLTFAQVQADPAFKASAAAI
                     AAGFVGLLSLFNIGGRIFWASLSDHIGRKATYAIFFLLGIALYAAAPSAAREGSIALF
                     VLCLCIILSMYGGGFATVPAYLADIFGTIYVG"
     gene            complement(1845..2042)
                     /locus_tag="VNR65_03685"
     CDS             complement(1845..2042)
                     /locus_tag="VNR65_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17810.1"
                     /translation="MLQWTTLASGRRFGLIVHHTDGEREYAYDRNSAFGRLDAALDAA
                     AANGWTVVDMKRDWKRVFKFQ"
     gene            complement(1997..>2548)
                     /locus_tag="VNR65_03690"
     CDS             complement(1997..>2548)
                     /locus_tag="VNR65_03690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWJ17811.1"
                     /translation="PEWKDKEPFMAVLEGDLKALAASGEKGLVQLLMETHAGMTTEQF
                     TQIVKDWLATARHPRFDRPYTELVYQPMIELLAYMRANGFKTFIVSGGGIEFMRPWTE
                     QIYGVPPEQVVGSSIKTKFQFKDGIPELFRLPDVHFIDDRPASRSASTSTSGGARSPH
                     SAIPMAILRCSNGPLWQAAAVSG"
BASE COUNT          618 a          762 c          690 g          478 t
ORIGIN      
        1 cgcccacata gatagtcccg aaaatatcgg cgagataggc aggaacagtc gcaaagccac
       61 cgccatacat cgacaggatg atgcacaagc ataagacgaa caatgcgatg cttccctcgc
      121 gcgcggcgga gggagctgcc gcgtaaaggg cgatgccaag caggaagaag atggcatagg
      181 ttgctttacg gccgatatgg tcggagagcg atgcccagaa gatgcggccg ccgatgttga
      241 acaacgagag gaggccgacg aagcctgcgg cgattgctgc agcggacgcc ttgaacgcgg
      301 gatcagcctg aacttgcgca aacgtcaaat ccggctttcc gattaaagcc ccgccgaaga
      361 tctcctgcag cataggcgaa gcgatgccga tcacgcctat ccctgccgat acgttcagca
      421 tgagcacaag ccatatgagc cagaatgagg tcgtcttata ggcatcgcgc acatgcacat
      481 ggtggaccgt gatcagcgac ttcttcttga gcggcggcgt ccacccgtct ggctgccagc
      541 catccggtgg cacgcgatag ccgaatgcgc cgcccagcat gaaaacgaaa tagatggccg
      601 ccagcgctag aaatgtttgc caaacgccta cgtctgctgg tgtctggaaa tgctgcatca
      661 gaataacggc aagcggagag ccgatcatcg cgcctccgcc aaaacccatg atcgccattc
      721 cggtagccat gccgcgcttg tccggaaacc atttgataag tgtagagacc ggcgagatgt
      781 agccgaggcc gagccctatg ccgccgatga tgccagagcc gagccacatc agccaaagct
      841 ggtgatagcg cacgcccagc gcggaaatac agagcccgcc gcaccaacaa aacgctgaaa
      901 cgatgcctgt ctttcttgga ccgacacgct ccacccatcc gccgaatacc gcggcggaga
      961 gaccgaggaa cacgaagaag agcgtgtaca tccagccgag gtcggccacg cgccagtcgc
     1021 aggttgtggt aaaaagcgcc tgcccgagtc ccatatcggg gcaagccacc ggggccttaa
     1081 ttccaagcgc tttcgacagc ggcagccaga acacgctgaa gccgtaagcc ataccgatgc
     1141 aaagatgaat ggcgagcgcc gccggaggaa caagccatcg gttgaatcca gttttggcga
     1201 tgatccttct gcggctcagc aggcccgcca cgcctgccgc cgcacttctc gcgctccctt
     1261 cgatggtcgc cattccttgg cttctcccat ttttaaaaac tgtctcgtat ttataacgcg
     1321 cctcaaccaa gcgctaatcc gtccggccgg gggcttcgag acttcacccg cagcaagtca
     1381 cagcaaaccg cgtcagagac actccatttt ttccagatca gaagaactga cgaaatcgat
     1441 cggagtaaat cctccccgag tggatcgcat tatgccactc gctgaagaaa acacgccaat
     1501 cccgccgcag attaccagat ttcgtagtcg aaatcctccg agcaaagcgt tccactgcgc
     1561 agaaaatctt aaaactaagc catcgatgcc cccacacaaa ggttgaaaca acagactttg
     1621 caactttaac acgaaaaaag ctccattcgg gaacagaatt aagctataat taactttttg
     1681 ctggcggctt gctgtctaac tgcagcttcc tacaaaagca gttccgcccc cagaaacgct
     1741 tcagcgccga agtagtttcg cgccgcaccc aaaggtctat gccgtgcgta ggtcatcctt
     1801 agatgtgcgg caaaggcggg gggcagccat tgccctgaat gagattactg gaacttgaag
     1861 acgcgtttcc aatcccgctt catgtcgacg accgtccatc cattcgcggc tgctgcatcc
     1921 aaggccgcat ccagccgacc aaaggccgag ttgcggtcat aggcgtattc gcgttcccca
     1981 tcggtgtgat ggacgatcag cccgaaacgg cggccgcttg ccagagtggt ccattggagc
     2041 atctcaagat cgccatcgga attgccgaat gcggcgatcg ggcgccgccc gatgtgctcg
     2101 ttgatgccga ccggcttgcg ggcctgtcgt cgataaagtg aacgtccggc aaccggaaca
     2161 gttctggaat gccgtccttg aactggaact tcgtcttgat gctcgatccg acgacctgct
     2221 cgggtggaac gccataaatc tgctccgtcc acggccgcat gaattcgatg ccgcctcctg
     2281 aaacgatgaa ggttttgaag ccgttcgcgc gcatataagc aagtagttcg atcatcggct
     2341 ggtacacgag ttcggtgtag ggacgatcga accggggatg ccgggcggtc gcgagccaat
     2401 ccttcacaat ctgagtaaat tgctcagtgg tcatacccgc atgagtttcc atgaggagct
     2461 gaaccagccc cttctcgccg gacgcggcca aggccttcag gtcgccttcg aggacagcca
     2521 tgaaaggttc cttgtccttc cactcagg
//
LOCUS       DATMGX010000228         2076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_110092, whole genome shotgun sequence.
ACCESSION   DATMGX010000228 DATMGX010000000
VERSION     DATMGX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110092"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..460)
                     /locus_tag="VNR65_03695"
     CDS             complement(152..460)
                     /locus_tag="VNR65_03695"
                     /inference="COORDINATES: protein motif:HMM:NF039461.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HWJ17812.1"
                     /translation="MERFFGGSPLLVILKLVTASLIIGVVLSFFGFNPNNLYHAVIRL
                     GDWISSLGFDAVKTGVRYFILGAFIVIPLWLLVRFFSFLGSGRRVERSEPPAPRRFKK
                     "
     gene            complement(614..904)
                     /locus_tag="VNR65_03700"
     CDS             complement(614..904)
                     /locus_tag="VNR65_03700"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HWJ17813.1"
                     /translation="MASQKKSGSTSNRGFASMDSEKQRAIASKGGQSVPNEKRSFSQN
                     RSLAAEAGRKGGQNVSDENRSFSRNRDLAAQAGRKGGQASHGGKAREDRSPV"
     gene            complement(1258..1671)
                     /locus_tag="VNR65_03705"
     CDS             complement(1258..1671)
                     /locus_tag="VNR65_03705"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWJ17814.1"
                     /translation="MRKVLLAACLLACGVFASAASAEVVVRVSKGRQAMYVYVDGQLA
                     YTWPVSTAAWGYNTPDGVYYPTALDAYHRSGRYGSPMPYSIFFTGGYAIHGSYDIRNL
                     GYPASHGCIRLHPQNAGELYDLVRSSNRTRIVVGW"
     gene            complement(1735..>2076)
                     /locus_tag="VNR65_03710"
     CDS             complement(1735..>2076)
                     /locus_tag="VNR65_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17815.1"
                     /translation="LRHITDRAPILLLAHEPDIFDRVPASITLTLAGHTHGGQIYIPF
                     VGRPAFFADNARYAKYAYGHIKKDGRHMIVSSGLGLSNIPIRFMVPPEIAIVTLRHGE
                     RDVKAPLIVRA"
BASE COUNT          484 a          569 c          585 g          438 t
ORIGIN      
        1 tatgacggca gtgtccgcca tacccacgag cggcaccgtc gcgttcgaca ggatcatcgg
       61 gatcgcgata gcgagcacat cgcggcttcg gagccgcttt ccctgcggtc tgtgttcgca
      121 caaaagcgcg gacctttagc cttgtgccct tttatttctt gaatctacgc ggcgccgggg
      181 gctcggatcg ttcgacccgg cgtccggacc caaggaagct gaagaagcgc accaggagcc
      241 aaagcggaat gacaataaat gcgccgagaa tgaaatagcg gactccggtc ttgacggcgt
      301 caaagccgag agaggagatc caatctccga gccggatgac ggcgtgatac agattattgg
      361 gattgaagcc gaagaaagag agcaccaccc cgataatcaa cgaggcggtc acaagcttca
      421 aaatcactag gagtgggctg ccgccgaaaa agcgttccat gatcaagtcc gtttctgcgc
      481 tcccacacgc atacccggca cttgagaaat cgcaaagaag aaattcgcga tccggtggag
      541 acgtgttgat atacggcgaa ccccgccggc atgacggggc tgcgcctaag acgcgtttgc
      601 cggctgaagg tctctaaact ggggaacgat cttcacgtgc tttaccgcca tgactcgctt
      661 gtcccccttt gcgtccggcc tgagcggcga gatcgcggtt gcgagaaaag ctgcggttct
      721 catcggaaac gttctggccg cccttacggc cggcttccgc cgccaatgag cggttttggc
      781 tgaagcttct cttttcattg gggacgcttt gcccaccctt ggatgcgatt gcccgttgct
      841 tctcagagtc cattgaggca aagccgcgat tcgaggtaga gccagatttc ttctgggagg
      901 ccattcaagc tcctatctta ctgtttttgg acgtagcctc tgactgagac tttacatccg
      961 ttaacagcgc gctcccgctc tggttccgct ataatgacta agtttcgcgt gttttttgcg
     1021 atcgtcggcc tgatctccct taggccgata cattcttgaa gcggtctagt tcagaaaaac
     1081 aaccttaggc tgtgtatcct gactggatct ctatataaac tgcttatata gattcttgct
     1141 gttttttgaa aaacaactcg agaatattga atgcgcgttg gatcgcaagg cggctcctga
     1201 agcgacaagc gggcacccag agcgcgcggc tggcgaactc aaaagctcag gccttcatta
     1261 ccacccaacg acgatcctcg tcctgttgga actgcggacc agatcgtaaa gctcgccggc
     1321 attctgggga tgaagccgga tgcagccgtg agatgcgggg taacccaaat tgcggatgtc
     1381 ataggagcca tggatcgcat agcctccggt aaagaagatc gagtacggca tcggagagcc
     1441 atatcgacct gaacggtggt aggcatcgag ggctgtggga taatacacgc cgtcgggtgt
     1501 gttatagccc caggccgcag tcgatacagg ccaagtatat gccagctgcc cgtcgacata
     1561 gacgtacatc gcctgccttc ctttgctgac acgcactaca acctcagcgg aggcggcgga
     1621 cgcaaaaacc ccgcaggcca gcaggcaagc ggcaagcaac accttacgca taaaaagccc
     1681 caagtctaca atgtgccccg gaaacaggcc ctgactgctt cctcattttt aagattacgc
     1741 cctaacaatt aagggagcct tgacgtctcg ttcaccatgg cgaagcgtta cgatcgcaat
     1801 ttcgggcggc accataaaac ggatgggaat gttcgagagg ccaaggccgc tggaaacgat
     1861 catatggcgc ccatctttct tgatatgacc gtaggcgtac ttggcataac gcgcattatc
     1921 cgcaaagaaa gccggccggc ccacaaatgg gatatagatt tggccgccgt gggtatggcc
     1981 cgcgagcgtc agtgtgatgc tggccggaac gcggtcaaaa atgtcgggct cgtgcgcgag
     2041 cagaaggatc ggcgcacggt cggtaatgtg gcgcag
//
LOCUS       DATMGX010000229         2238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_200964, whole genome shotgun sequence.
ACCESSION   DATMGX010000229 DATMGX010000000
VERSION     DATMGX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200964"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1798
                     /locus_tag="VNR65_03715"
     CDS             248..1798
                     /locus_tag="VNR65_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HWJ17816.1"
                     /translation="MRQVSMTTRDELVAAIAGRYAWSGRLEKGRILDEFVAVTKLHRK
                     HAQRLLRQGNARQRSAPRPGRRIYDEAAREALILVWESSDRICGKRLKPLVPILIEAM
                     ERHGHLRLANEVRTRLLSMSAATIDRSLREVREQAGGRKRRRAVASASLRRSVPVRTF
                     SDWNDPPPGFFEADLVAHSGPRANGSFIQTLVLTDIATGWTECMPLLFREQTLLTEVL
                     SQMRVRLPIDVRGLDTDNDSVFMNTTVKDYCVANRIEFTRSRPYRKNDQAWVEQKNGA
                     IVRRIVGYRRFEGLQAAAELSRLYDSVRLFVNYFQPSFKLAEKQRDGAQVRKRYHKPA
                     TPYQRLLDDPRTSAEVRLQVASVCATLDPVRLLHDIRASQQKLVEIADTPAATEAGSI
                     CPPTIDQFLANLKTAWREGEANPTAKAKAKVPRSRRRPDPLASVNQQIESWFAAEPWR
                     TAREFLDRLQAEHPQLYRDGHLRTLQRRLKVLRRKAAQSLVLGSTSHPNESGRHREDL
                     DIREPALS"
     gene            2079..>2238
                     /locus_tag="VNR65_03720"
     CDS             2079..>2238
                     /locus_tag="VNR65_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ17817.1"
                     /translation="MQITTIGLDLAKNVFQVHGVDAEGRVVVRRKLRRAKVREFFAGL
                     APCLVGIEA"
BASE COUNT          484 a          644 c          684 g          426 t
ORIGIN      
        1 ccgccctgga ccgcgaagcc ataggaattg ctggtcaagg ccgggaaagc ataggctggc
       61 gtggaacccg cgactgtagc cgccggcgtg gtaagagcag aggccccgaa aaggctggca
      121 ttgacctgat gcgccgccgc cgacaattgc accgcgcccc gcattgcctc gcctgaaatg
      181 ttgctgaata taaacctgtt gcctcatctg atttgctcct tttgggcgtc agggagcata
      241 ggcggatatg aggcaggtga gcatgacgac acgggatgaa cttgtggcag cgatagcggg
      301 ccgatacgcg tggagcggcc gtttggaaaa gggccggatc ctcgacgagt tcgttgcggt
      361 aacaaagctg caccgcaagc acgcgcagcg cctgctgagg caaggcaatg cacgccagcg
      421 atcggctcct cgcccaggac gcaggattta tgacgaagcg gcgcgtgagg cgttgatctt
      481 ggtttgggag tcttccgatc ggatctgcgg caaacggctc aagcctcttg tgccgattct
      541 catcgaggcg atggagcggc atggccatct cagacttgcg aatgaggttc gcacgcgcct
      601 cctctcaatg agcgccgcta cgattgatcg atcgctgcgg gaggtcaggg agcaagccgg
      661 aggacgtaaa cggcgccggg cggtcgcgtc ggcttccctg cggcgaagcg tgccggtgag
      721 aacattttct gactggaacg atcccccacc ggggttcttc gaagctgact tggtcgctca
      781 cagcggtccg cgggcaaacg gcagctttat ccaaaccttg gtgctcactg acatcgccac
      841 cgggtggacc gaatgcatgc cgcttctctt tcgggaacaa accttgctga ccgaagtgct
      901 gtcgcaaatg cgggtgcggc tgcccattga tgttcgcggg ttagatacgg acaacgatag
      961 cgttttcatg aacacgacgg tcaaggacta ttgcgtagcg aaccggatcg agttcacgcg
     1021 ttcgcggccc taccgtaaga acgatcaagc ttgggtcgaa caaaagaacg gcgccatcgt
     1081 ccggcgcatc gtcggctatc ggcggttcga aggcctgcag gccgccgccg aattgagccg
     1141 tctctacgat tcggtgcggc tcttcgtgaa ctacttccaa ccttccttca aacttgctga
     1201 aaagcagcgt gacggcgcgc aagtccgcaa gcgctaccat aagccggcaa cgccatatca
     1261 gcgattgctg gatgatccga gaacctcggc ggaagtccgt cttcaagtcg cttcagtttg
     1321 cgcgaccctc gatccagtcc ggctcctgca tgatattcgg gccagccagc agaagcttgt
     1381 cgaaatcgcc gacacgccag ccgcaacgga agcaggatca atctgtccgc caacgatcga
     1441 ccagtttctt gccaacttga agacggcatg gcgggagggc gaagccaatc caaccgctaa
     1501 ggccaaagca aaagttccac gttcgcgccg acgacccgat ccgctggcat cggtcaatca
     1561 gcagattgaa agctggtttg cggcggaacc ctggcgaacc gcccgcgagt tcctcgatcg
     1621 cctacaggct gaacatccac agctctatcg agacggacac cttcgtacgc tacagcgacg
     1681 gctcaaagtt ctgcggcgga aagcagccca aagtctcgtc ctcgggtcga ccagccatcc
     1741 caatgaatcc ggcaggcacc gcgaagatct ggacattagg gaaccggcac tctcgtaaaa
     1801 ggccactggg acgcaagccg ctcgctcgct acggcgcaat ggaaagcgcg cgtcgcgagc
     1861 ggcttgcttc gggggaagcg cactgagagt gtaaggctga acccgcgtcg gccgggagca
     1921 tttatgatga ggcaacaaac aggcgttcgg gaacatttct ccctgaggca atacggattc
     1981 tcatcttgat tgatggggtg gacgccccct gctccccggc atccaggtgc catatggtgg
     2041 tcatcgtgaa tcggccacag tcgagaggag gcgtccaaat gcaaattacc acgatcggat
     2101 tagacttggc gaaaaacgtg tttcaggttc acggcgtcga tgctgaaggc agggtcgtgg
     2161 ttcgtcgcaa attgcgtcgt gcgaaggtaa gggagttctt cgcggggctc gccccgtgtc
     2221 tcgttggaat agaagctt
//
LOCUS       DATMGX010000230         2400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_6607,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000230 DATMGX010000000
VERSION     DATMGX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6607"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VNR65_03725"
     CDS             <1..398
                     /locus_tag="VNR65_03725"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWJ17818.1"
                     /translation="PALKAGLNFIRLTAPTTNDHRLGSVLANSSGFVYYVSIAGVTGT
                     RAPDLNAVARNVARIKARTPLPVAVGFGVRTPEQARTISSVADGVVVGSALVDALSQS
                     LDENQRATDLTIRAVLGLVEELSAGMRAG"
     gene            439..1467
                     /gene="accD"
                     /locus_tag="VNR65_03730"
     CDS             439..1467
                     /gene="accD"
                     /locus_tag="VNR65_03730"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HWJ17819.1"
                     /translation="MNWINSVVRPKISGLWPKRDMPGDLWVKCPETGQMVFHKDLEAN
                     QFVIPGSGYHMRMGADARLKSFFDGGKFETVATPDVPADPLKFRDEKRYSDRIKEARA
                     KTGMQDALLTGIGTLDGLSVVGAVQDFAFMGGSLGMAAGEAIITGMLKAVELRVPYIL
                     FVSSGGARMQEGILSLMQMPRTTVAVQRMRDAKLPYIVVLTNPTTGGVTASYAMLGDI
                     HIAEPGALIGFAGPRVIEQTIREKLPDGFQRSEYLLDHGMIDMVVHRHDMRATLSRLC
                     SLLGTERKHRAGMNNVKQKAAANSGASPEIRVPATAEQLAAASNKSKPGSEDIAEEVV
                     PPAPANPS"
     gene            1519..>2400
                     /locus_tag="VNR65_03735"
     CDS             1519..>2400
                     /locus_tag="VNR65_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWJ17820.1"
                     /translation="MHPQNKTDSLLARFETLHPKSIDLSLGRVQRLLSVLGDPHRRIP
                     PVIHVAGTNGKGSVVAFLKAMLEAAGYRTHAFISPHLRRFHERIMLAGQGGGKAIGER
                     HLADILSRAEAANRGQPITFFEITTAAAFVAFAENPADIVLLETGLGGRLDATNVIDE
                     PLATILTPISLDHCSYLGDTIEAIASEKAGILKRGRPAIVSRQSDSAFGVIEARADAL
                     SSPLHAGGRTWDVFEQQGRLIFQDEDCLLDLPLPRLFGRHQLENAGAAIAAVRSLEGF
                     HVSEEAIAKGITAAVWPA"
BASE COUNT          471 a          723 c          746 g          460 t
ORIGIN      
        1 ttcctgcctt gaaggctggc ctgaatttca ttcggctgac ggctccgaca acgaatgatc
       61 acaggctcgg cagcgtgctg gcgaatagca gcggcttcgt ctactacgtg tccatcgccg
      121 gggtaaccgg gacgagggcg ccagacctca acgcggtcgc gcgaaatgtc gcgcggatca
      181 aggcgcgcac gccattgcct gtcgccgttg gcttcggagt ccgcacgcca gaacaggcac
      241 gcaccatctc aagcgtggcc gatggcgtgg ttgtagggtc ggcgctcgtc gatgcacttt
      301 cacaaagtct tgacgagaac cagcgggcga ccgacttaac cataagggcg gttctggggc
      361 tcgtcgaaga gctctccgca ggcatgaggg cgggttaggc acacggcctt ttccgaggca
      421 caaaaaggcg ttagcgaagt gaactggatt aatagcgttg ttcggccgaa gatcagcggc
      481 ctgtggccaa aacgggacat gcccggcgat ctttgggtaa agtgccccga gaccggacaa
      541 atggtcttcc acaaagacct cgaggccaat cagttcgtca ttcccggctc cggctaccac
      601 atgcggatgg gagccgacgc gcggctgaag tcctttttcg acggcgggaa gtttgaaacg
      661 gtggcaacgc cggatgtccc cgctgaccct ttgaagtttc gcgatgagaa gcgctattcg
      721 gatcgcatca aggaagcgcg cgccaagaca ggcatgcaag acgccctcct gacggggatc
      781 ggcacccttg acggcctttc ggtggtcggc gcggttcagg atttcgcctt catgggcggc
      841 tcgctcggca tggcggccgg cgaagcgatc atcacgggga tgctgaaggc ggttgagttg
      901 agggtccctt atatcctgtt cgtttcctcg ggtggcgccc gcatgcagga gggcatcctg
      961 tccctcatgc agatgccgcg cacgactgta gccgtccagc gcatgcgcga tgcgaagctg
     1021 ccctatatcg tagttttgac caatcccacg acgggcggcg tgacggcgtc ttacgcgatg
     1081 ctcggcgaca ttcacatcgc ggagcctggc gctctgatcg gctttgccgg ccctcgcgtg
     1141 atcgagcaga cgatccgcga aaagcttccg gacggcttcc agagatcgga atatctcctt
     1201 gaccacggca tgatcgacat ggtggtgcat cgccacgata tgcgcgctac gctcagccga
     1261 ttgtgcagtc tcctgggcac cgagcggaag caccgcgcgg gcatgaacaa cgtgaagcag
     1321 aaggcggcag ccaattcggg cgcttcgccc gaaatcagag tgcccgcaac ggccgaacaa
     1381 cttgccgccg cttctaacaa aagcaagccg ggatccgaag acatcgccga ggaggtggtg
     1441 ccgcctgcgc ccgccaaccc atcctgatga gcccgcgatt tcgccgacgc gcgtcaagct
     1501 ttagcaccgg acaaagcctt gcatcctcag aataaaactg acagcctgct cgcgcgcttc
     1561 gaaacgctgc atccaaagag catcgacctt tcgctcgggc gcgtgcaacg ccttctgtcc
     1621 gtgctaggag acccgcatcg gcgcatcccc ccggtcattc atgttgctgg cacgaatggc
     1681 aaaggttcgg tggttgcctt tctaaaggcg atgcttgaag cggcaggcta tcgcacgcac
     1741 gcgttcatct cgccccattt gcgccggttc cacgagcgga tcatgctggc ggggcagggc
     1801 ggcgggaagg ccatcggtga gcggcacctc gccgacatcc tgagccgcgc cgaggctgcc
     1861 aatcgcgggc aaccgatcac cttcttcgag attacgactg ccgcagcatt tgtggccttc
     1921 gcggaaaatc cggcggacat tgtccttctc gaaacaggac tcggcggaag gctcgatgca
     1981 acgaatgtga ttgatgagcc tctcgccacc atcctgacgc cgatttcgct cgatcattgc
     2041 tcgtatcttg gcgacacaat cgaggccatt gcgagtgaga aagccggcat cctcaagcgt
     2101 ggacggccgg ccatcgtatc gcggcaaagc gacagtgcgt tcggtgtgat cgaagcgcgc
     2161 gcagacgcgc tgtcgtcgcc tttgcacgcg ggtggccgaa cctgggatgt tttcgaacag
     2221 cagggcaggc ttattttcca ggatgaagat tgcctgctgg atttgccact gccgcggttg
     2281 ttcggacgtc atcaactcga gaatgccggt gcggcgattg ccgcggtgag atccctcgaa
     2341 ggctttcacg tctccgaaga ggcgattgcc aagggcatca cggccgctgt ctggccagcg
//
LOCUS       DATMGX010000231         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_173262, whole genome shotgun sequence.
ACCESSION   DATMGX010000231 DATMGX010000000
VERSION     DATMGX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173262"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VNR65_03740"
     CDS             complement(<1..1135)
                     /locus_tag="VNR65_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWJ17821.1"
                     /translation="MPPRRALAESRSPLWFAIQRIKTALSPNVTITPAPPSILAQWDV
                     AITMRDGVTLRANVFRPKSDARVPVIMSAHPYGKDKIAARTRSGRSPNLQYRLFPQPD
                     PIAISEWTSWEAPDPAVLVPRGYAVFNCDLRGAGTSEGVGDFFSEIEGRDYHDLIEWA
                     ADQPWSNGRVGLDGVSYLAISQYFAAVQHPPHLAAICPWEGFNDAYRDFVYPGGVREN
                     GFSKLWFHKAGQARVKTNLEIEANRHTDRDAFWAERTAPIEKIEVPMLVCASFSDHSL
                     HSRGSFEAYRRAASGQKWITTHRSGKWSTYYGQDATETRLRFFDHFLKGADNGWDREP
                     AVRLAIYDTGSDPAAVTKEEAWPPNDLTWQRLWLGATGMALADA"
     gene            1692..>2051
                     /locus_tag="VNR65_03745"
     CDS             1692..>2051
                     /locus_tag="VNR65_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWJ17822.1"
                     /translation="MRSSHAERLRALIAAEILSFKLRPGEKLDESRLADKYGTSRTPV
                     REALRQLASDGLIQIRHNRGAIVAKVGIRELVELLEVMAELEGACGRLAAKASSFQAL
                     DATRAAQESCRKYAELRN"
BASE COUNT          414 a          596 c          627 g          414 t
ORIGIN      
        1 gggcgtctgc cagggccata cccgttgctc caagccaaag gcgctgccag gtgagatcgt
       61 ttggtggcca ggcctcttcc ttggtcacag cagcgggatc gcttcctgtg tcatagattg
      121 cgagacgaac agcgggctcc cgatcccagc cattgtcggc gcctttcagg aaatgatcga
      181 aaaagcgaag gcgggtctct gtcgcatcct gaccgtaata cgtgctccat ttgccggagc
      241 gatgcgtggt gatccatttt tgcccggacg ctgcgcgccg gtaggcctcg aaactgccgc
      301 gcgagtgcag tgaatgatcc gaaaagcttg cgcaaaccag catgggcacc tcgatctttt
      361 cgataggcgc cgtgcgctct gcccaaaagg cgtcgcgatc ggtatgccgg ttcgcctcaa
      421 tttcgagatt ggtcttgacg cgcgcctgcc cggctttgtg aaaccagagc ttggagaatc
      481 cattctcgcg aacgccgccc ggatacacga aatcccggta cgcatcgttg aacccctccc
      541 acgggcagat cgccgccaga tgcggagggt gctgaacggc agcgaaatac tggctgatgg
      601 cgaggtagga cacgccatca agtccgacgc gtccgttgga ccatggctgg tcggcggccc
      661 attcgatcag gtcgtggtag tcgcgtcctt cgatctcgga aaagaagtcg ccaacgccct
      721 ccgacgtccc cgcgcccctc agatcacaat tgaagacggc gtaaccgcgc ggcacaagaa
      781 cggctggatc gggcgcttcc cagcttgtcc attccgaaat ggcaatcggg tcgggctgag
      841 ggaaaaggcg gtattgcagg ttcgggctgc gtccgcttcg agttcgcgcc gcgattttat
      901 ccttcccgta agggtgggcc gacatgataa ccggaacgcg cgcatcgctt ttcggccgga
      961 acacattcgc gcgcagcgtc acgccatccc gcattgtgat tgccacatcc cattgcgcca
     1021 ggatggatgg aggcgcgggt gtgatcgtca cattggggct caaagcggtc ttgatgcgct
     1081 gtatcgcaaa ccataacgga ctgcggcttt cggcaagcgc acgtcgtggg ggcatctcac
     1141 atctctccca tcagggccgt cggaggcgga cggtccaaaa ttatgcttgg agggcactat
     1201 gacggtgctt cccttgggcg aggcctgacg cccagcttat gctttggaaa tcaactcgat
     1261 ttcgatatcg gaggcagcgg agatcagagc tttgcgcgac ggtctcatcc cggaccgtct
     1321 tagcggtctc ccccggattg gggattttca gcgacgtaat aatatgccct tggtccactc
     1381 ttctccgggg gatgcgtaac cttcgccatt cgcgcctatc aattgatcac cgagggttta
     1441 catggacggc gaacattcgg caggttccgt ccaagactct gacagcgacg atttgaggtc
     1501 ccgggcaggt ggccgctcag gcgtgctgcc aaaggctccg gcaggattca gcagcgccga
     1561 aacagattga gccagagtgc gtccggaaaa gtgcgaagcg gttttccgat aaagacgctc
     1621 gacaaaacaa aaacctagag cccgctcttg attgcatcag gagcggatag gctctatgag
     1681 gagcgggcga tatgaggtcg tcacatgccg agcgtctgcg cgcactgatc gcggcggaga
     1741 ttctctcatt caagctgagg ccgggggaga agcttgacga gtccaggctc gcggacaagt
     1801 acggcacgtc tcgcacgccc gttcgggaag ccctccggca gcttgcctcc gacggattga
     1861 tccagatccg gcataataga ggcgcgatcg tcgccaaggt cggcattcga gagcttgtgg
     1921 aacttcttga agtcatggcc gaactcgaag gcgcttgcgg gcgtctcgcc gcgaaagcga
     1981 gctcgtttca agcgcttgat gcaactcgtg ctgcgcagga gtcttgccgc aaatacgctg
     2041 aactgcgcaa c
//
LOCUS       DATMGX010000232         2597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_332601, whole genome shotgun sequence.
ACCESSION   DATMGX010000232 DATMGX010000000
VERSION     DATMGX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332601"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VNR65_03750"
     CDS             complement(<1..221)
                     /locus_tag="VNR65_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17823.1"
                     /translation="MVDLLARAREEEYGEGRLIEPSPDRIDRWLEAAGAWLMRHQRTI
                     QAVQWVVVAAYALLVIVPAFLPPPQRAAH"
     gene            complement(362..694)
                     /locus_tag="VNR65_03755"
     CDS             complement(362..694)
                     /locus_tag="VNR65_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWJ17824.1"
                     /translation="MKRVALILSLLCLCMSSSGRAGDEPVFQIEMNDGVITPSRIEVP
                     AKTRFKIIVKNTGKMPAEFESTELRKEVVVPAGSEAPLIIRTLDVGEYKFFDDFQPDA
                     PPALLIAK"
     gene            complement(694..1236)
                     /locus_tag="VNR65_03760"
     CDS             complement(694..1236)
                     /locus_tag="VNR65_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HWJ17825.1"
                     /translation="MDIRIAGIVACAMCSMASGAWSKEYPIGQAQEKEGMHIAAVYLQ
                     PIEMDPPGMMRDAKESDVHLEADIKAAKGNPNGFAEGDWMPYLRVGFELVKLDDKQTL
                     KGEFMPMVANDGPHYGDNVKLFGPGKYKLSLTISPPSENTQAHFGRHVDKETGVGPWF
                     KTFNVDYEFVYAGTGKKGAY"
     gene            1583..1852
                     /locus_tag="VNR65_03765"
     CDS             1583..1852
                     /locus_tag="VNR65_03765"
                     /inference="COORDINATES: protein motif:HMM:NF016280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HWJ17826.1"
                     /translation="MTQTTGRFFDEIAKLMTDAAGAAQGARSEIQTAARSQAERFFRS
                     MDLPQREEFEAVKAMALKAREENEKLAKRITELEAALGRTQGGTS"
     gene            2081..2584
                     /locus_tag="VNR65_03770"
     CDS             2081..2584
                     /locus_tag="VNR65_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HWJ17827.1"
                     /translation="MDAAGQEQDYNRVSHPVDLMERIACLQDWVFERSSEDELTVSVA
                     GSWCDYHISFNWRDDLESLHMACAFDFKVPRNRTTEIYRLLVLINEQLWLGHFDIWSS
                     EGLLLYRQGLMLNGAEPTAKQCEALLQAALDTCERYYQAFQFVVWAGKRAEDALASTI
                     FQTEGQA"
BASE COUNT          570 a          734 c          725 g          568 t
ORIGIN      
        1 atatgcgcgg cgcgttgcgg tggcggcaga aaggccggaa cgatgacgag aagtgcgtag
       61 gcagcgacaa caacccattg aacggcctgg atcgtccgct ggtgccgcat gagccaagcg
      121 cccgcagcct ccagccagcg atcgatgcgg tctggcgagg gctcgatcaa acggccttcg
      181 ccatactctt cctctcgggc gcgggccaga agatcaacca tggaaccata atccagtcat
      241 agcgtgagcc aggtgtttct cggcccgata atcgaaaaaa ggcgcggtgt ctgcaagacg
      301 actccaagga aacaaacacc gcgccagtac ccttgccgcc gttctcgcgg ccgggcatgc
      361 ctcatttcgc gatcagcagc gccggtggcg cgtcaggctg aaaatcgtcg aaaaacttgt
      421 attcgccaac gtcgagcgtg cgaatgatca ggggcgcctc gctgccagct ggaacgacga
      481 cttctttgcg aagttccgtg ctctcgaatt cggcgggcat cttccccgta ttcttcacga
      541 tgatcttgaa cctcgtcttg gccggcacct cgatccgcga cggggttatg acgccatcgt
      601 tcatctcaat ctggaagacc ggctcatctc cggcccgacc agacgaggac atacacaggc
      661 agagcaggct tagaataagc gcaacccgct tcatcaatac gcgcccttct tgccggttcc
      721 ggcgtaaacg aactcgtagt cgacattgaa ggtcttgaac cacggaccca cgcccgtttc
      781 cttgtccaca tgcctgccga agtgggcctg tgtgttttcg cttggcgggg agattgtcaa
      841 actgagcttg tatttgcctg ggccgaagag cttgacatta tcgccatagt gggggccgtc
      901 attggccacc atgggcatga actcgccctt cagggtctgc ttgtcatcga gcttgaccag
      961 ctcaaaaccg acccgcaggt agggcatcca atcgccctcg gcaaatccgt tcggattgcc
     1021 cttcgccgcc ttgatatccg cttcgagatg aacgtccgac tccttcgcgt cgcgcatcat
     1081 tcctggcggg tccatctcga taggctgaag ataaaccgcc gcgatgtgca tgccctcttt
     1141 ctcctgggct tgccctatag ggtattcttt gctccaggcg ccgcttgcca tgctgcacat
     1201 cgcacaggcg acaatgccgg caattcttat atccattcct tcgtgctccc ccagacgcgg
     1261 ctttttcctg gcaatgaaac cgcttcccaa ttatcgttta ctcttaatgt ttggaacaga
     1321 accacccgat ttacttatag ctagcttgtg agtatacaaa tggcaatgcc tttcttttta
     1381 aattagatca gttctaaagt gagttcggtg cgctagtact gtcttgtgta ggaataagct
     1441 tgcaatattg agtggggctg gcggcaatcg cccggtcaca acgcttgctg cgtcaaatgc
     1501 aacaacgcta tgcgtcggct tgcaggagca gagcgcaatg attagtttgc cgcacgagca
     1561 aggcatacga ggaaaccgat ccatgacgca gaccacaggc cgttttttcg acgagatcgc
     1621 gaagctgatg accgacgccg ccggcgccgc acaaggtgcg cgcagcgaga tccaaaccgc
     1681 cgcgcgcagc caggccgagc gcttctttcg aagcatggat cttccgcagc gcgaagagtt
     1741 cgaagcggtg aaggccatgg ccttgaaggc acgcgaagaa aacgagaagt tggcaaagcg
     1801 aatcactgag ctcgaggcgg ctctgggccg gacgcaaggc ggaacatcgt gacaatctta
     1861 gattgaaaag cgatttacgc aactgcttgg caacaccacc gttaataatg ccaagtcaac
     1921 gatgttttcc ccgtggagaa agttgcggat cgattgcgca aaacctgcgg atcaccgcat
     1981 agcctcttgt gacttgatgc ggcgagaccc gaacagataa agcgttgcga ccgaaaggtc
     2041 tgtcggaaat tttgctgcgc catcagaggg gaaattacgg atggacgctg ccggacagga
     2101 acaggattac aatcgtgtct cacaccctgt cgatcttatg gagcgtattg cttgtttgca
     2161 ggactgggtc ttcgagcgtt cgagtgaaga cgagttgact gtatccgtgg cgggttcgtg
     2221 gtgcgattat catatctcgt tcaattggcg tgatgacttg gaaagcctgc acatggcgtg
     2281 cgccttcgac ttcaaggtgc ctcgcaaccg aacgacggag atttaccgtc tcctcgtcct
     2341 catcaacgaa caactctggc tcggccactt cgacatatgg tcatccgaag ggctccttct
     2401 ctatcgtcag gggttgatgc tgaatggcgc ggagccgacc gccaagcaat gcgaggcgct
     2461 actccaggcc gccttggata cttgcgagcg gtattaccag gctttccagt tcgtggtctg
     2521 ggccggtaag cgcgccgagg atgcgttggc gagcactatt ttccagaccg aggggcaagc
     2581 ttgaccggaa gcatcga
//
LOCUS       DATMGX010000233         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_331759, whole genome shotgun sequence.
ACCESSION   DATMGX010000233 DATMGX010000000
VERSION     DATMGX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331759"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VNR65_03775"
     CDS             <1..423
                     /locus_tag="VNR65_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWJ17828.1"
                     /translation="LSVVDRIKAGIEAVHEIVGNVWPWIIAGIAAGAFIHGYVPADLL
                     ASIMGRDVWWSVPAVVLIGIPMYSNAAGIIPVVTALLAKGAALGTVLAFMMSVIALSL
                     PEIIILRKVLSVKLIAVFVGVVGTGILAVGFLFNALFQ"
     gene            441..677
                     /locus_tag="VNR65_03780"
     CDS             441..677
                     /locus_tag="VNR65_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWJ17829.1"
                     /translation="MKDVKVLGPGCKRCVTTAEMVQAAADKLGVPVKVEKVTDYAAIA
                     GYGIASTPGIVIDGKVVHAGGLPKPDDLARWLAA"
     gene            679..1092
                     /locus_tag="VNR65_03785"
     CDS             679..1092
                     /locus_tag="VNR65_03785"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWJ17830.1"
                     /translation="MAPMLEYRVSARRIDSHGSIASTKNAEVILDTDISGRPDAFNPA
                     ELFLASIAACMIKGIERVTPMLKFDLRGVEVKLYGVRQDSPPKMIAVDYELIIDTDET
                     DQRLELLHTNVRKYGTISNTVAEATKLEGTIRRRP"
     gene            complement(1287..1511)
                     /locus_tag="VNR65_03790"
     CDS             complement(1287..1511)
                     /locus_tag="VNR65_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17831.1"
                     /translation="MSLPTILVGFTRNSRDQSFSVLGKNWVFLLVMAAGSIVGAFIGG
                     HLLGLVANRVLLPLLAALLMISAVKVWRHR"
     gene            complement(1508..2092)
                     /locus_tag="VNR65_03795"
     CDS             complement(1508..2092)
                     /locus_tag="VNR65_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HWJ17832.1"
                     /translation="MSETSRTSWRREPLAFVSGAAIGALGGLIGLGGAEFRLPLLITL
                     FQFRGLEAVILNKAISLVVVAAALPFRASTISFAELGTQWPIIVNLLAGSVIGAWIGA
                     SWATRLRSETLYRVIAVLLVLVAAILLFAHDTAGGTPLFVGWIQIIAGIIAGFIIGVI
                     ASLLGVAGGEFLIPTLVLLFGADILSLPVASRWP"
     gene            complement(2254..>2475)
                     /locus_tag="VNR65_03800"
     CDS             complement(2254..>2475)
                     /locus_tag="VNR65_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ17833.1"
                     /translation="MFPEQSDETASGLEMGSLEDVRQSAERRHILRALAATSGEVGAA
                     SKLLGIGRTTLWEKMRRLGLNAVDLDVDL"
BASE COUNT          554 a          739 c          714 g          468 t
ORIGIN      
        1 ctctcggtcg tcgatcgcat caaggcggga attgaagcag tccacgaaat cgtcggtaac
       61 gtttggccgt ggatcatcgc gggcatcgcc gccggcgcct ttattcacgg ttatgtgccg
      121 gcggacctgc tggcctcgat catggggcgg gacgtctggt ggtcggtccc ggccgtggtt
      181 ctgatcggca ttcccatgta ttcgaatgcc gcaggcatca ttcccgtggt cacggcgctg
      241 ctcgcaaaag gcgcagcact cgggacggtg ctcgcgttca tgatgtcggt gattgcactg
      301 tctctgcctg agataatcat tcttcgcaaa gtactcagcg taaagctgat cgcggtcttc
      361 gtcggtgtcg tcgggacggg catcctggcg gttggtttcc tgttcaacgc cctgttccaa
      421 tgagctaacg gagaacattc atgaaagacg tcaaagtcct cggccctggc tgcaagcgct
      481 gcgtcacgac ggccgagatg gtgcaggccg cagccgacaa gctcggcgtt ccggtcaagg
      541 tcgagaaggt gaccgattat gccgcgatcg ccggctacgg catcgcttcg acgccgggca
      601 tcgttatcga tggcaaggtg gtccatgcgg gcggtctgcc taagccggac gatctggcgc
      661 gctggctcgc ggcctgaaat ggcgccgatg ctggaatatc gagtcagcgc caggcggatc
      721 gattcccatg gcagcatcgc ctctaccaaa aacgccgaag tcattctcga cacggacatc
      781 agcggccggc cggatgcctt taacccggcg gagctgtttc ttgcctccat cgccgcctgc
      841 atgatcaagg gcatcgagcg ggtgactccg atgctcaaat tcgatctgcg cggcgtggaa
      901 gtgaagctct acggtgtccg gcaagacagc ccgccaaaga tgattgcggt cgactacgaa
      961 ctcatcattg atactgacga aaccgaccaa cgccttgagc tcctacatac caacgtccgc
     1021 aaatatggta cgatctcgaa cactgtggcc gaagcaacaa agcttgaagg tacgatccgg
     1081 aggagaccat gatcttttca actctggacg cagcacattg atcgatctcg atccgcaagc
     1141 acccaagccg ctggttgcgg tgttaacagc acagaaacaa ggcgcacctc gaagggccga
     1201 gccgaaacta ccgacaccgg ccgtgcgcgg ccaaacagat tcgacgcgaa tgacgcgttt
     1261 cgaacattca accccagtat cggtggctat cggtgccgcc atactttcac tgccgagatc
     1321 atgagcagcg cggccagcag cggaagcaag acgcgattgg cgacaaggcc aagaagatgg
     1381 cccccgataa aagcgccgac gatggacccg gctgccatta ccaataagaa cacccaattc
     1441 tttccgagta ccgagaagct ttgatcccgg ctgttgcgtg tgaagccgac aagaatggtt
     1501 gggaggctca cggccagcga gaggctaccg gcaagcttaa tatatcggcc ccgaataaca
     1561 gaaccagcgt cgggattaga aactcaccac cggctacgcc aagaagcgaa gcaatcaccc
     1621 caataatgaa cccggcaatg atgccagcga tgatctggat ccatccgacg aagagcggcg
     1681 tgccacccgc ggtgtcatgc gcgaacaata gaattgccgc gaccaagacc aaaagtacgg
     1741 cgatgacgcg ataaagcgtt tccgatctca ggcgcgttgc ccagctcgcg ccaatccaag
     1801 ctcctatgac gctgcccgcc agcaagttca cgatgatcgg ccattgggtg ccaagctcgg
     1861 caaacgatat tgtgcttgct cggaacggaa gcgctgccgc gacaacaacg aggcttatgg
     1921 ccttgttgag tatgacggcc tctaaccctc ggaattgaaa cagggtgata agcaacggca
     1981 agcgaaattc tgccccgcca agacctatca gcccgcccag ggcaccgatt gcggctccag
     2041 atacgaaagc cagaggctcc cgccgccacg atgttcgaga ggtttcagac atagcgccac
     2101 ggcgaagcga cgccttagcg tctagggggg tggccgcgat ccgtcgtgcc ggtgtgcttc
     2161 acgacctgct tacggcaggc cgtggggtgc atgggagttt agccacaaac atagcaatgt
     2221 ctaggcaggc ctgccccgtg caaaagggtg ccttcataaa tcgacgtcga ggtcgaccgc
     2281 gttcagtcca agacgacgca tcttttccca tagcgtcgta cgcccgatcc caagcagctt
     2341 ggaagcggcg ccgacttctc cgcttgtcgc cgccagcgcg cgcaggatat ggcgcctttc
     2401 tgcactctgt cggacgtctt cgagcgatcc catttccaaa ccagaggcgg tctcgtcgct
     2461 ctgttccgga aacat
//
LOCUS       DATMGX010000234         2265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_238852, whole genome shotgun sequence.
ACCESSION   DATMGX010000234 DATMGX010000000
VERSION     DATMGX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238852"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(368..781)
                     /locus_tag="VNR65_03805"
     CDS             complement(368..781)
                     /locus_tag="VNR65_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17834.1"
                     /translation="MSNAQDSLKVDPLSQIGEAIESATGSLGDAASNASASAKVAARK
                     VKSTARSGVYNAAYGLSYGVVFGAVFLVELLPEDTLVRRGFEDGAEDGFDAAIAKSAA
                     RRSKRRISHKEEEEELKLDEESEGGQTNHPETKHP"
     gene            1016..>2265
                     /locus_tag="VNR65_03810"
     CDS             1016..>2265
                     /locus_tag="VNR65_03810"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HWJ17835.1"
                     /translation="MNQRSRSGAVSLNSVAVLGGKLGIALILCLALPAVLVAQDKPVD
                     DIGLINPEAAAHAFPKHGFSPYAGRNYPTRVFWGDEHVHTGWSVDAGAFGATLGPEEA
                     LRFARGEQVMSSLGEPAKLSRPLDWAAITDHSDAAGTIFEIRDGNPNLMTDPQLRKWH
                     DMMVSGKGVEAASEMIRAQSNNRVPAGLKDPSLALSVWKKNTSIMEKYNEPGRFTAFI
                     GYEWTSNAGGGDNLHRNVIYRDGKDKADQVLPMTTFDSENPEDLWKWMAAWEEKTGGA
                     LLAIPHNGNLSNGKMFALQTFIGNPLTREWAETRARWEPMYEATQIKGDGETHPSLSP
                     SDEFANFEKWDQANLAGVPKQPGMIEREYARRALQEGLKLEQSLGVNPFKFGLAGGTD
                     SHTGLTTAEEDNFFGKHTGVEPSP"
BASE COUNT          458 a          670 c          687 g          450 t
ORIGIN      
        1 tcgatgtgcc ccgagcggtg atctcggcca agggctggcc gaaggtgtgt gagatcgtag
       61 catcggccag gaaccgcgcc acagagtcca ctaacccgct gttcatgcga cttcttcatt
      121 tcaaaatgta aagagttccc agctagtgcg cttccatcaa cgtaaccagt aatgactggg
      181 gtccgaggtg agccaacccc acgcatcgag gaggatgctt cttgtaggcc cgctgaccga
      241 ggcccagctg gctgaacagc gtccctttcc ggtcatccga tcccgcgcac ccctgacttc
      301 ccgaactcct tcggggagtg aatttccacc catcagcgcg gcgcgaaccg attcttcatt
      361 ctccccgcta gggatgcttg gtctcgggat ggttcgtctg ccccccttcg gactcttcgt
      421 cgagcttgag ttcctcttcc tcttctttgt gggagattcg cctcttggaa cggcgcgcag
      481 ccgacttggc gatggccgcg tcgaaaccat cctctgctcc atcctcgaag ccgcgacgca
      541 cgagggtgtc ctctggaagc aactcgacga ggaaaacggc gccgaaaacg actccgtagg
      601 ataggccata ggccgcgttg taaaccccgg accgggccgt gctcttgacc ttgcgggcgg
      661 cgactttagc cgacgcagat gcgttgctcg cggcgtcacc cagcgagccg gtcgcggatt
      721 caatggcctc cccgatttgc gagagcgggt cgaccttgag agagtcttgc gcattagaca
      781 tcgtgattgt ctcctttttt tgatatagat cgatttctta gagtgtcgaa ggcgacgtca
      841 ccggttccgt cttggctcct ccgttttcat tgacgacatt gacgatggtc ttggggtagt
      901 ggcggggctc tagaggacat cgcgacagag ttctcaagac gctctcgcgg agcggaaacc
      961 aattttttac ctacgtaatc tcgggcgtgc aaaaaaaaac gtaagggagc agacgatgaa
     1021 ccagcggagc cgttccggag cggttagcct gaattccgtt gcagttttgg ggggcaaact
     1081 cggtatcgcg ctgatcttat gcctcgctct gcctgcggtc ttggtggcgc aggacaagcc
     1141 ggtggacgac atcgggctta tcaatccgga ggccgccgcg catgccttcc ccaagcacgg
     1201 cttttccccc tatgccggtc gtaactatcc gacgcgagta ttctggggcg acgagcatgt
     1261 gcacaccggc tggtccgtcg acgcgggcgc tttcggcgca acgctcgggc cggaggaggc
     1321 gctgcgtttc gcgaggggtg agcaggtcat gtcctcgctc ggcgagccgg cgaagctgag
     1381 tcggccgcta gattgggccg cgatcaccga ccattccgac gccgcgggca ctatctttga
     1441 aatccgggac ggcaatccca acctcatgac cgatcctcag ctgaggaagt ggcacgacat
     1501 gatggtctca ggcaaaggcg tcgaggccgc ttccgaaatg atccgcgcgc agtccaacaa
     1561 ccgcgtgccg gcggggctca aggaccccag tctcgccttg tccgtctgga agaagaacac
     1621 ttccatcatg gaaaaataca acgagcccgg acggttcacc gctttcattg gctacgagtg
     1681 gaccagcaat gctggcggcg gcgataactt gcatcgcaac gtcatctacc gcgatggcaa
     1741 ggacaaggcc gatcaggtcc tgccgatgac gaccttcgac agcgaaaacc cagaggattt
     1801 gtggaaatgg atggcagcat gggaggagaa gactggcggt gcgcttcttg ccatccccca
     1861 caacggcaac ctgtcaaacg gcaagatgtt tgcgcttcag acgttcatcg gcaatccgct
     1921 gacgcgggaa tgggccgaga cgcgggcgcg ctgggaaccg atgtacgagg ccacccagat
     1981 caagggtgac ggagagacgc atccgtcgct ctcgcccagc gacgagttcg ccaatttcga
     2041 gaaatgggat caggcaaatt tggcaggcgt cccgaagcag cccgggatga ttgagcgtga
     2101 atatgcgcgt cgcgcgttgc aggaaggtct gaaacttgaa cagtcgctcg gcgtgaaccc
     2161 gttcaagttc ggcctcgcgg gcggcaccga cagccacaca ggtctcacga ccgcggagga
     2221 ggataacttc ttcggcaagc atacgggcgt ggagccgtcg ccgca
//
LOCUS       DATMGX010000235         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_109650, whole genome shotgun sequence.
ACCESSION   DATMGX010000235 DATMGX010000000
VERSION     DATMGX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109650"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VNR65_03815"
     CDS             <1..429
                     /locus_tag="VNR65_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17836.1"
                     /translation="HDIGWYSIDMKTIIEQGFRRGNFLQSDARYLHESESVRLGTEVL
                     RGNGWDEATIAAVCEIIDGHDTRTNPHHLNDRIMRDADKLWRYEVTGVSVASDWFGVT
                     PHNYTDLIEAQLERFETDHGRAMAEAELAVTRDKLMLHVL"
     gene            851..1498
                     /locus_tag="VNR65_03820"
     CDS             851..1498
                     /locus_tag="VNR65_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWJ17837.1"
                     /translation="MTDKSIFLPRDLPLWEEAKGFLDVRNNDEHTVVAYALARALQVE
                     IPQADESVVLPAILLHDVGWKRIPEELLLLAIGRKPTRLDLVRDHEIHSVEIAREILE
                     RHRLQGVDIEAVLEIIDGHDTVKEAKNINDAVVKDADKGWRATPHGMRTIAGWYDVPF
                     SEVILMLQERSNPLMLTVPGRVLAEANTAAVEAELMIENYLGHGPQFKEVKPRRR"
     gene            1500..>2183
                     /locus_tag="VNR65_03825"
     CDS             1500..>2183
                     /locus_tag="VNR65_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ17838.1"
                     /translation="MVKDLGLAGKRVIVTGAAGGLGRAFAESFAEAGAVVMAADITAT
                     GAGETVALVREVGGKAHAGVVDVTRLESCQALCEAARATMGGVDALINNAAVYAGLER
                     RPFEEIDEAVWDNVMAVNIKGVWQMTRAVAPLMRQVGSGSIINIASATVFSGSPQWMH
                     YVASKGAVIAMTRVMARELGGDNIRANVIAPGFTLTEASFGHMADARSYGADRAALNR
                     NAEVDDITGG"
BASE COUNT          459 a          622 c          693 g          409 t
ORIGIN      
        1 cacgatatcg gctggtactc catcgacatg aaaacaatca tcgagcaggg tttccgccgc
       61 ggcaattttc ttcagtccga tgcacgctac ttgcatgaaa gcgagagtgt tcgtctggga
      121 actgaagtcc ttcgagggaa cggctgggac gaagcgacga ttgccgccgt ctgcgagatc
      181 atcgacgggc atgacacgcg gacaaacccg catcacctga atgaccgtat catgcgcgac
      241 gctgacaagc tgtggcgcta cgaagtgacg ggcgtctcgg tggcttccga ctggttcggc
      301 gttacaccgc acaattatac agacctgatc gaggcgcaat tggaaagatt cgaaacagac
      361 cacgggcgcg cgatggcgga ggctgagctg gccgtaacgc gtgacaagct catgctccac
      421 gtgctctgag gtcgaccaat gaagaaatat tcatcgccag cggatggaag ccggcgggat
      481 tcggcgcaac cccgcaagtg atcaatcggc gcaccgatag gccttcgatg cggttccggc
      541 cggcggcgac gcggatgctg acgcggccct cgctgccgcc tcgcgccttc gaaaactgga
      601 agcgcacaca cgatgattga cagggtgcgc atccagaaag caatggtgca agaaatgatc
      661 tcgcccgggg ctcgatgccc tgcgagggca tcgaggcaac ctcggtttgt ctcgatgaca
      721 tccagcattc gtcccgctac ctgcccttcg acggcatgaa ggagagcgat cccgggcgcg
      781 agaacggacg ttatggtatt gaatcatacc tcgcgtacaa gaggcgttgt ctgagttacg
      841 aggtatccca gtgaccgata aatccatatt tctgcctcgt gacctgccgc tctgggagga
      901 agcgaaaggc tttctggacg tacgcaacaa cgacgaacat accgtagttg cctatgctct
      961 ggcgcgggcg ttgcaggtgg aaattcccca agccgacgaa tccgtcgtct tgccggcgat
     1021 cctgctgcat gatgttggct ggaagcgtat ccccgaggaa ttgctgctgc tcgccatcgg
     1081 ccgcaagccg actcgacttg acctcgtgcg tgaccatgag atccatagcg tcgagatagc
     1141 acgtgaaatc ctcgaacgtc accgcctgca aggcgttgac atcgaagccg tgctcgagat
     1201 catcgatggt cacgacacag taaaggaagc caagaacatc aatgacgccg ttgtcaaaga
     1261 cgccgacaag ggctggcgcg ccacgccgca cggcatgcgt accatcgctg gctggtacga
     1321 tgtcccgttt tcggaagtga tcctgatgct gcaggagcgc tcgaacccgc tcatgttgac
     1381 tgtccccggc cgggtgctcg cggaagccaa caccgctgcg gtagaggcgg agctcatgat
     1441 tgagaactat ctgggccacg gcccgcaatt caaggaagtg aagccgcgaa ggaggtgaag
     1501 tggtgaagga tcttggtctt gccggaaaac gcgtgatcgt caccggcgcg gcgggtgggc
     1561 ttggccgcgc ctttgcggag tcctttgcgg aggctggcgc cgtggtcatg gccgccgaca
     1621 tcactgcgac cggcgctggc gaaaccgtcg cgctggtccg tgaggtgggt ggcaaggcgc
     1681 acgccggtgt ggtggacgtg acccggttgg agagctgtca ggcgctctgc gaggctgcgc
     1741 gagccacgat gggtggtgtt gatgcgctaa tcaacaacgc tgcggtctat gccggactgg
     1801 agcgccgacc cttcgaggag atcgacgaag ccgtatggga caacgtaatg gcggtcaaca
     1861 tcaagggcgt ctggcaaatg acccgcgctg ttgcgcctct tatgcgtcag gtcggcagcg
     1921 gttcgatcat caacatcgcc tcggccacgg tgttcagcgg ctcgcctcaa tggatgcatt
     1981 acgtcgcctc caaaggcgcg gtgatcgcaa tgactcgggt gatggccagg gaactcggcg
     2041 gtgacaacat ccgtgccaat gtcattgcgc ctggtttcac gctgaccgag gccagcttcg
     2101 gccacatggc cgatgcgcgc agctatggcg cggaccgcgc ggcgctgaat cgcaacgcag
     2161 aagttgacga cataactggt ggt
//
LOCUS       DATMGX010000236         2919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_199200, whole genome shotgun sequence.
ACCESSION   DATMGX010000236 DATMGX010000000
VERSION     DATMGX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199200"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="VNR65_03830"
     CDS             complement(<1..1413)
                     /locus_tag="VNR65_03830"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17839.1"
                     /translation="MSDKRRISEKRRLAEKQEKPEKAVSPAEKPLPGLQSPEEFVTNL
                     MRSVEATGQVIAKIVEDKDKRHGAFTVANGMADSGKLFAPILQYWMNDPKGYSEAQAK
                     LSEDMIELWGRTYKRFIGEKVEPMIAPSAHDPRFRDKEWTENAFFDFLKQAYLLGGKW
                     AEEMVHNASTVDAHIKHRAEFYLNQIVSALSPSNFPFTNPEVIRATVSTGGKNLAEGM
                     THLMEDLDHSGELLRVRQTDMSAFEVGKNLAVTPGKVVYQNELLQLIQYSPTTETVHA
                     VPMLIVPPWINKYYILDLTPPKSFIRWLVGQGFTVFVISWVNPDERLSSKTFEDYMLE
                     GVMAGVDAVLRITGQPYTNTLGYCVGGTLLACTLAYMAANDDHRINSATLFTAQTDFT
                     KAGDLLVFIDDEQLKALEDLMAEKGFLDGARMAAVFNALRPKDLIWPYIVNNYLLGKQ
                     PFPFDLLYWNSDSTRMPPLNH"
     gene            complement(1537..2256)
                     /gene="lexA"
                     /locus_tag="VNR65_03835"
     CDS             complement(1537..2256)
                     /gene="lexA"
                     /locus_tag="VNR65_03835"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWJ17840.1"
                     /translation="MLTPKQKQLLLFINERIEATGVPPSFDEMKDALELKSKSGIHRL
                     IMALEERGFIRRLPHRARALEVMKMPERGRNVFDADRRRGFMPTVIEGTRRSSFEESP
                     PAAFIRDREDGGIQVPVMGRIAAGVPRSAIEDHSHALVMPPEFLSQGEHFALEVRGDS
                     MIDAGIQEGDTVILKRTPTAESGEIVVALIDDEEATLKRLRKKGQSIALEPANPAFET
                     RIFGPDRVKIQGKLVALLRRY"
     gene            complement(2360..>2919)
                     /locus_tag="VNR65_03840"
     CDS             complement(2360..>2919)
                     /locus_tag="VNR65_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWJ17841.1"
                     /translation="AWGGYPLLLDIARDSKESLAACVRDGQKADVILTIGGASVGEHD
                     LVRGTLEDMGARFEVLRVAMRPGKPAMFGFLRNQRILSMPGNPAAAIICARVFLKPLL
                     DRFLGRSRRDEPSIVRLAVPVEANGEREHYMRAQLSEDGVAPIGDQDSSLMNAFAKAD
                     CLLVRPVHGPALPSGALVPVIPLDF"
BASE COUNT          598 a          872 c          829 g          620 t
ORIGIN      
        1 gtgattaaga ggcggcatcc gcgtcgaatc cgagttccag taaaggaggt cgaatgggaa
       61 cggctgcttg cccagcaggt aattgttgac aatgtagggc cagatcaggt ctttcgggcg
      121 cagcgcgttg aacaccgccg ccatccgtgc cccatccagg aacccttttt ccgccatcag
      181 gtcctcaagc gccttcagct gctcgtcatc gatgaagacg agcaaatcac cggctttcgt
      241 aaagtcggtc tgggccgtaa aaagcgtggc tgagttgata cggtggtcgt cgttagccgc
      301 catgtaggca agcgtgcacg caagcaaggt tccgcccacg cagtaaccga gcgtgttcgt
      361 atacggctgg ccggtgatgc gcagcaccgc gtcgacgccc gccataaccc cctcgagcat
      421 gtagtcttcg aaggtttttg aagaaagcct ctcatctggg tttacccatg agattacaaa
      481 gaccgtgaag ccctggccga caagccacct tatgaaggac ttgggtggcg tgaggtccag
      541 gatatagtat ttgttgatcc atggcggcac gatgagcatc ggcactgcat ggacagtctc
      601 ggtcgtcggc gagtactgaa tgagctgtag aagttcgttc tgatagacca ccttcccagg
      661 cgtcaccgcg aggttcttgc ccacttcgaa cgccgacatg tccgtctgcc ggacccgaag
      721 aagctcaccg gaatggtcga ggtcttccat aaggtgagtc atgccttcgg caagattttt
      781 tccccccgtt gataccgttg cgcggatcac ttcggggttt gtaaagggaa aattcgacgg
      841 cgagagcgcg ctcacaatct ggttcagata aaactcggca cggtgtttga tgtgggcgtc
      901 gaccgtggac gcgttgtgaa ccatttcctc ggcccacttg ccccccagca aataggcctg
      961 cttcagaaaa tcgaagaagg cgttttctgt ccattcctta tctctgaacc ggggatcgtg
     1021 cgccgaaggc gcgatcatcg gttcgacctt ctcgccaata aagcgcttat aggtgcggcc
     1081 ccacagctcg atcatatcct cactgagctt tgcctgggct tcgctatagc ctttcggatc
     1141 attcatccaa tattggagaa ttggggcgaa caacttcccg gagtcggcca tgccgttcgc
     1201 gaccgtaaac gcgccgtggc gcttatcctt atcctcgaca atctttgcga tgacctggcc
     1261 ggtcgcctcc acggatcgca tcagattggt gacgaattcc tccggcgact gcaaaccggg
     1321 caaaggtttc tccgctgggc tgaccgcctt ctccggcttt tcctgttttt cagccagccg
     1381 gcgtttttcc gatattctgc gcttatcaga cattcggcgc ttcctcgttc ccaacctgca
     1441 aagtcattga atgcttctcg cccggtacgc aacccaggca tcaacgcgaa tatagcactc
     1501 ttggcgttgt ttgatgagag gctccgccgc tctcttttaa tagcggcgaa gaagagcgac
     1561 gagcttacct tggattttca cccggtccgg tccgaaaatc cgggtctcga atgccggatt
     1621 ggcgggctcg agggcaatcg attgtccctt tttacgcaag cgcttaagcg tggcctcctc
     1681 gtcgtcgatg agagcgacca cgatctcacc gctttcagcc gtcggggttc gtttcaggat
     1741 gacggtatcg ccctcctgaa tgcccgcatc gatcattgaa tcgccgcgaa cctcgagcgc
     1801 gaagtgctca ccctgggata ggaactccgg cggcatgaca agggcgtggc tgtgatcctc
     1861 gatcgcggaa cggggcacgc ccgccgcgat ccgccccatg accggaacct gaatgccgcc
     1921 gtcttcacgg tcacggatga acgccgccgg ggggctttcc tcgaacgacg agcgtcgcgt
     1981 tccctcgata accgtcggca tgaaaccgcg ccgcctatcg gcatcgaata cattccgccc
     2041 gcgctcgggc attttcatga cttccagcgc acgggcgcga tggggaagcc tccttatgaa
     2101 accgcgctcc tcaagtgcca tgattaggcg gtgaatgccc gatttcgact tgagctccag
     2161 cgcatctttc atctcgtcaa acgacggcgg aacgccggtt gcctcgatac gctcattgat
     2221 gaaaagaagc agttgctttt gctttggcgt cagcattaca gccccaaata gagtgccgaa
     2281 tccggcttgt acaaaccggg aacatcaata agcgttctct ttgcgttccg caagggtaaa
     2341 acgcctcggc aagacgatgt cagaaatcga gagggataac aggcacaagc gctcctgacg
     2401 gcagggctgg gccgtgcacc ggccgcacaa gaagacaatc cgcctttgcg aaggcgttca
     2461 tgagcgagct gtcctgatcg ccgatggggg ccacgccatc ctcggaaagc tgcgcccgca
     2521 tgtaatgctc acgctccccg ttcgcttcaa cagggacggc gagccgcaca atgctcggtt
     2581 cgtcgcggcg cgagcgccca agaaaccggt cgagcaacgg cttcaaaaaa acgcgggcgc
     2641 agatgattgc ggcggcggga ttgcccggca tgctcaggat gcgctggttg cgcagaaagc
     2701 cgaacatcgc aggcttgccc gggcgcatcg ctacgcgcag cacctcgaac cgagcgccca
     2761 tatcttcaag cgtgccgcga accagatcgt gctcgccgac agatgcgccg ccgatggtga
     2821 ggatgacgtc ggccttttgc ccgtcgcgaa cgcaagccgc gaggctttcc ttgctgtccc
     2881 gcgcgatgtc gagaagaagc gggtagccgc cccaggctg
//
LOCUS       DATMGX010000237         5212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_321892, whole genome shotgun sequence.
ACCESSION   DATMGX010000237 DATMGX010000000
VERSION     DATMGX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321892"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VNR65_03845"
     CDS             complement(<1..565)
                     /locus_tag="VNR65_03845"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HWJ17842.1"
                     /translation="MTIPSLFLSHGAPTLPLTDTPARAFLQQLGLALERPKAILVISA
                     HWETTVPTVNAVDSNETIHDFGGFPRALYDLRYSAPGSPTLAARVAAQLRAGGFDCNI
                     DKRRGLDHGAWVPLLLMYPQADIPVLELSVQPHLGPEHHLRVGRALAPLRDEGVLIIG
                     SGSFTHDLSEFRGHGPNDPAPDWVNSFA"
     gene            complement(617..1057)
                     /locus_tag="VNR65_03850"
     CDS             complement(617..1057)
                     /locus_tag="VNR65_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWJ17843.1"
                     /translation="MTDTRTDHYAALLLRVSLGALFLAHASLKLFVFTPAGTAQFFGS
                     VGLPPALAYVTITAELLGGIALVLGLWTRVVAIALTPILLGAIFTVHGTAGFFFDNAH
                     GGWEYPAFWIIALIVQAMLGDGAYALHTSRNVDSIANLTGAYSR"
     gene            1160..2083
                     /locus_tag="VNR65_03855"
     CDS             1160..2083
                     /locus_tag="VNR65_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ17844.1"
                     /translation="MLDRLTGLEVFANVATAGSLSAAGRAMGISQTMVTKHIAALEAR
                     LGVKLFHRNTRRLSITEAGRNYLEASERILADIEAAEAAVTADRVEARGLLRLNAPVA
                     FGARQIAPLLSEFAQRHPLVTVELGLNDRLVDLADEGWNLAIRIGSLSDSSLIARRVA
                     PCRTVVCAAPSYLAARGRPRTVSSLAAHNCLGYTLSSMTGVDRWAFGAKGDTTVSVSG
                     NLRANNGEALRTAAIAGQGLAYLPTFIVADDMRAGTLVALGLDQPTVEFGGIYAVFLP
                     DRHPAAKVRAFIDFIANRFAPEAPWDQKLET"
     gene            complement(2267..3988)
                     /locus_tag="VNR65_03860"
     CDS             complement(2267..3988)
                     /locus_tag="VNR65_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ17845.1"
                     /translation="MSAPIGDDKHSDYSKYAPKRLREQPPLPAAERPYVTSAPTVPSP
                     TPMHQDRRATAEFASWPEPIPEPPPQLLEDTNVALIGRIAVVVTFAAAVALLLIFAKP
                     LWQDARALFNADAQRLQGAKSDRLTAYRPLVPATGTAAVSGAEPPASAQAQQAPLSPI
                     SHPLGQEPAAPSASLQVQQTPLSPTSHPLGQEQRSVNSPIRGVTDTEIRFGISAPFSG
                     PARELGQNMKLGIEAAFNVANASGGVHGRQLRLIAADDGYEPTRTAETLKRLYERDQV
                     FGLVGNVGTPTAAVAVPYALEHKMLFFGAFTGAGLLRSDPPDRYVFNYRASYAEETEA
                     IVHYLVKVRRLKPEEIAVFAQQDSYGDAGYAGVAKAIRSLPEGDESTILRLNYTRNTV
                     DVDEAVAQLRKKRRIPIKAVIMVPTYRAAAKFIEKTRDLYPDMIYTSVSFVGSTALAG
                     ELMLLGKRYATGVIVTQVVPAVDGHSSLVLDYKSALAKYFPGEAPDYVSLEGYVTANV
                     LIEALKRSGRQLDAERLVETLENLHDFDIGLGTPLTFGRSEHQGVHKVWGSQLDATGH
                     YQPIDLQ"
     gene            complement(4632..4793)
                     /locus_tag="VNR65_03865"
     CDS             complement(4632..4793)
                     /locus_tag="VNR65_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HWJ17846.1"
                     /translation="MSKLIAFLKNDSGATAIEYGLIAAGISVAIIAVVNGLGTQLNAK
                     FGSISSQLK"
BASE COUNT         1080 a         1510 c         1559 g         1063 t
ORIGIN      
        1 cggcgaaact gttgacccaa tccggtgccg gatcgttcgg accgtggcca cgaaattccg
       61 aaagatcatg cgtgaagctg ccgctgccga tgatcagtac gccttcgtcc cgcagcggcg
      121 ccaacgcgcg ccccacgcga agatggtgct ctggacccag atgcggctgg accgagagct
      181 caagcacagg aatgtcggcc tgcgggtaca tcaacagtag cggtacccag gcgccatgat
      241 ctaagccccg gcgtttatcg atgttgcaat cgaatccacc cgcacgcaac tgcgccgcga
      301 cgcgcgctgc caatgttggc gagcctggtg cagagtagcg caggtcatag agcgctcgcg
      361 gaaatccgcc gaagtcgtgg atcgtctcgt tgctatcgac cgcgttgacc gtcggcaccg
      421 ttgtctccca atgcgcggaa atcacgagaa tggccttggg acgttcaagc gcaagtccga
      481 gctgctgcaa gaacgcgcgt gccggcgtgt cggtaagcgg taacgtcggc gcgccgtgcg
      541 acaggaacag actgggaata gtcatgacat gtcctccggg gattgcaggg ccggcatatc
      601 ggccccgccg gctcgtttag cgtgagtatg cgccggtcaa gttggctatt gagtcaacat
      661 tgcgcgacgt gtgtaacgca taagcaccgt cgccaagcat ggcctgtacg atcaacgcga
      721 tgatccagaa tgccggatat tcccagccgc catgcgcatt gtcgaaaaag aacccggcag
      781 ttccgtgcac cgtaaaaata gcgccaagta aaatcggcgt aagagcaatc gctacgaccc
      841 gcgtccagag cccaaggacc agcgcgatgc cgccgagaag ttcggctgta atagtgacat
      901 acgcgagcgc gggcggcagg ccgacactgc cgaagaactg cgccgttcca gcaggggtga
      961 aaacgaacaa tttgaggctg gcgtgcgcaa ggaaaagcgc gcctaggctg acgcgaagca
     1021 gcagcgcggc atagtggtcg gttcgggtat cggtcatggg tagctcctgt gtttgccgcc
     1081 cagatatggc ctattcacca cggtgtgata atataggctt tacgaattac agatcacacc
     1141 acggagatga ggttggggca tgcttgaccg cctgaccggg ctggaagtgt tcgcgaatgt
     1201 cgccaccgca ggcagcctct ctgccgccgg gcgcgccatg ggcatatcgc agactatggt
     1261 gaccaaacac attgccgcgc tcgaagcccg gctgggcgta aagctctttc accgcaacac
     1321 tcgacgtctt tcgatcaccg aggccggacg caactacctc gaggcgtccg aacgtattct
     1381 cgccgacatc gaggcggcag aagccgccgt caccgctgat cgcgtcgagg cgaggggatt
     1441 acttcgcctg aatgcgccgg ttgcattcgg tgcgcggcag atcgcccctt tgctctcgga
     1501 gttcgcacag cgtcatcccc tcgtgactgt tgaactcggc ctcaacgacc gattagtcga
     1561 cctcgccgat gagggctgga atcttgcgat ccggatcggc agcctcagcg actcaagcct
     1621 aattgcgcga cgggttgcgc catgccgcac cgtcgtctgc gccgcgccgt cttatctcgc
     1681 ggcgcgtggc agaccgcgca cggtttcgag cttggcggcc cataattgcc ttggatacac
     1741 gctctcaagc atgaccggcg tcgatcgatg ggcgttcggg gcaaaggggg acaccaccgt
     1801 ctccgtttca ggtaatctac gagccaacaa tggcgaagcc ctgcgaacag ccgcaatcgc
     1861 aggccagggg ctcgcctacc taccgacttt tatcgttgcc gacgacatgc gcgcggggac
     1921 actggtcgcg ctgggcttgg atcaaccgac agtggaattc ggcgggatct atgccgtctt
     1981 cctgccggat cgtcatcccg ctgcaaaagt gcgcgcgttc atcgatttca tcgctaaccg
     2041 ctttgcaccc gaagcaccct gggaccaaaa gctcgaaaca tgaatgtcgg cttgtgatgc
     2101 tgtggacggc tcctccaccg gcacgtgagt gccatgaatg tgggtgctgc taagaccccc
     2161 acaatttgga ggagccaaaa atgcagactg gaatgacacc gccatgtcct tgcaaaactt
     2221 ttctttagcg ttcagataaa agtatcgctg cgacggcaaa ccgccgctac tgcaggtcaa
     2281 tgggttgata atgccccgtc gcgtcaagct gtgaacccca taccttgtgc acgccctggt
     2341 gctccgatcg gccgaaggtc aaaggcgtcc caagcccaat gtcgaagtcg tggagattct
     2401 cgagagtttc caccagtctt tcggcatcga gctgacggcc actccgtttc aacgcttcga
     2461 tcaacacgtt ggccgttacg taaccttcga gggacacgta gtccggagcc tctccaggga
     2521 agtatttggc gagcgcagat ttgtaatcga ggaccaacga cgagtggcca tcgacggccg
     2581 gcacaacctg cgtcacgatg accccagttg catacctttt gcccaggagc atcaactcac
     2641 cggccaacgc ggtgctgccc acgaaagaaa cgctggtgta aatcatgtct ggatagagat
     2701 cgcgtgtctt ctcgatgaat ttcgccgcgg cccgataggt gggcaccatt atcaccgctt
     2761 ttattgggat gcggcgcttc tttcgcagtt gcgcaacggc ctcgtccaca tccacggtgt
     2821 tgcgcgtgta attgagccgc aaaattgtgc tctcgtcgcc ctccggcagc gaacggatag
     2881 ccttggctac accggcgtat cccgcgtctc cgtacgagtc ctgctgggca aaaacggcga
     2941 tctcctccgg ctttagccgc ctcaccttca ccaaatagtg cactatggct tcggtctctt
     3001 cagcgtagct ggctcgatag ttgaaaacat aacggtcggg tggatcgctc ctaagtaagc
     3061 cggcgccggt aaacgccccg aaaaacagca tcttatgctc aagggcgtag ggcactgcga
     3121 ctgcggctgt gggcgtcccc acgttgccaa ctaatccaaa aacttgatcg cgctcgtaga
     3181 ggcgcttcaa tgtttcggca gtccgagtgg gctcgtagcc atcgtccgcc gcgatgagcc
     3241 ggagctgccg gccgtgcacc ccgccactgg cgttggccac gttgaacgca gcctcgatgc
     3301 ccagtttcat gttctggccg agttccctcg ccggaccact gaaaggtgcg gaaatcccaa
     3361 accgaatttc agtgtcggtt accccgcgaa tgggactgtt gacgctcctt tgttcttgcc
     3421 cgagtggatg cgaggtcggt gagagcggcg tttgctgaac ctgcaagctc gcggacggcg
     3481 ccgctggttc ttgcccgagc ggatgtgaga tcggtgagag cggcgcttgc tgtgcctgcg
     3541 cgcttgcggg cggctccgct ccagagaccg ctgcagtccc tgtggctgga acgagtgggc
     3601 gatacgctgt gagtcggtca gatttggcgc cttgtaatct ctgcgcatct gcattgaaca
     3661 gcgcgcgtgc atcttgccat agcggcttcg cgaagatgag cagcaatgcg acggcagccg
     3721 caaatgtgac caccactgca atccgcccta tcagtgctac gttcgtgtcc tccagcaatt
     3781 gtggtggtgg ctctgggatg ggttccggcc atgaagcgaa ttcggccgtt gcccgccggt
     3841 cctgatgcat gggcgtgggc gaaggaaccg tgggtgccga agtaacgtaa ggtcgttcgg
     3901 cagcaggcag cggcggctgc tcgcgaagtc gtttaggcgc atacttacta tagtcagaat
     3961 gcttgtcgtc gccgatcgga gcactcatta cgcattccta ttcccagcgt ccgcgccggt
     4021 gcttcaagtg ctgaggctcc tattcaaatg tggcgcctgc gtgtcttgac cggagctatg
     4081 catttgcggc gctgcaacat catggcagca attgggaaca gaccggacgt gccgcggaca
     4141 tcgcgaaacc gacgcggttg tggtcctgga gtgcggctgc gagagcgcca gcgaccaaaa
     4201 aagccaaagt aggggcgtcg cgcttcggcg gccctgcctc aaggcggcga tcaacgaaac
     4261 gaacccaatt gggtttgggt tcatcgacga gaacggcggc ggtgtgggtg tgcgaatccg
     4321 caatcgtcag gggccaaagc agcccaaata gtcgtcctcg gtgctcttcg atcctgatgc
     4381 ggatccaatc agccctcgaa caggccggga tcgtctttct agatagcgat gcgagtggtg
     4441 gcgttggcgt gcggctgaag aaggagtcgc cgcgaggtca atttttgaat ttgcgaaacg
     4501 aagccaaatt tcgctcatgg cttcgaccgt ctctcggttc cacccgtcga acgcctgcgg
     4561 cccattccaa agagagaggc cgccaacata aggcggcccc tgtattctaa gcaaggcttg
     4621 ccaggttgac tttatttgag ctggctcgaa atgctgccga acttggcgtt caactgggtg
     4681 ccaaggccgt tgacgaccgc gatgatcgcg acggaaatcc cggcggcgat caggccgtat
     4741 tcaatagcgg tagcgccaga atcattcttc agaaatgcga tcaacttaga catggctgag
     4801 ctccacggtt ggggttggca tatgaagagc ttatgattca aaatttaaga gtaagtacca
     4861 cgcatctgta atttctgggg ggtgactgta tacgttatat taatcgtgtt ttgtgaccgc
     4921 ctgcgatgtc cgtcatgggt cagcgacgtc gtgcgttttt tggagaaaga gcccgccaag
     4981 tgaggcggct cgaaaaaacc ccggcctttc gaccggggtt tctgcaacac aacctagatc
     5041 ggtaagagaa ttcgcccccg atctgagtgg gatgttagag tgcgacatat tagcaatgaa
     5101 tgaatcgagg ctaagaagcc gcggtcgcag cgagcgccaa agctcatcgg gtgatgcgcg
     5161 cggccagcac ggctttctga aacagcgcgt tcatcgcctg cgatatcccg tc
//
LOCUS       DATMGX010000238         2022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_111149, whole genome shotgun sequence.
ACCESSION   DATMGX010000238 DATMGX010000000
VERSION     DATMGX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111149"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /gene="istA"
                     /locus_tag="VNR65_03870"
     CDS             complement(<1..1213)
                     /gene="istA"
                     /locus_tag="VNR65_03870"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HWJ17847.1"
                     /translation="MLTTDAINDLHHLYWTERWPIRKIELHLRMSWRTIKKYLEAPAQ
                     TPATRSRTSKLDPFKPTIAELLEKDARASAAVIEQRLRPLGYDGGHTILRDYIHNVRP
                     QPRPKRAFVRMEPEAGERFEADWGHFGTLSYEGDQRKLYAFALVDGHSRMSYLEFTHS
                     QCFETFVRCHIHAFTAFNGVSREIYYDNLATAVAEHDGRLVRFHPRFLGFAREYNFVP
                     RACNQASGWEKGKVERGGVGYVRQNFWPLREFTDLHDVNRQARQWLAEIANVRLHHET
                     RERPIDRFRADALRPLPVIPYDYRDTVEALVHKDLRLQFDGNRYCVPHRFVGHRLTVK
                     GDASSVAIYHRYQEIVSYARSWRRGKTFGAERFERELAEQRPAARRSQAQQRFLGFLD
                     GLCAPDVAEGYL"
     gene            complement(1696..>2022)
                     /locus_tag="VNR65_03875"
     CDS             complement(1696..>2022)
                     /locus_tag="VNR65_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17848.1"
                     /translation="FPPGTSKWNKIEHRLFSFISKNWRGQPLTSLKVIVNLIAGTTTK
                     KGLKVHAEIDGQSYPAGIKVPDSKMADVSAGIKQQHFRRFCSSKEQTHWRGRGTHSKS
                     VSKLLA"
BASE COUNT          372 a          583 c          631 g          436 t
ORIGIN      
        1 gcaaataacc ctcggctacg tcgggggcgc agaggccgtc cagaaacccg aggaagcgtt
       61 gctgcgcctg ggaacggcgg gccgccggcc gttgttctgc cagttcccgt tcgaagcgct
      121 cggcgccgaa agtcttgccg cgccgccaac tgcgcgcgta agagacgatt tcctgatagc
      181 gatggtagat ggcgacggaa ctggcatcgc ctttcaccgt gagccggtgc ccgacaaagc
      241 ggtgtgggac gcagtagcga ttgccatcaa actgcaaacg gagatctttg tggaccaggg
      301 cctcgacggt gtcacggtaa tcgtaaggga tcaccggtag cgggcgtagg gcatcggcgc
      361 ggaagcgatc gatgggtctc tcacgggttt catgatggag gcgcacgttg gcgatctcgg
      421 ccagccactg tcgcgcctgg cggttcacat cgtgcaggtc ggtgaactcg cgcaacggcc
      481 agaaattctg gcgcacgtat ccgacgccgc cgcgttccac tttccctttt tcccagccac
      541 tagcctgatt gcaggcgcgc gggacaaagt tgtattcgcg ggcaaagccc aggaaacgcg
      601 ggtggaagcg caccaggcgg ccatcatgtt cggccacggc cgtggccaga ttgtcatagt
      661 aaatttcccg gctcacaccg ttgaatgccg taaatgcatg aatgtggcat cgtacgaaag
      721 tctcgaaaca ttggctgtgg gtgaactcca gatacgacat ccggctgtgc ccatcgacca
      781 acgcgaaggc atacagttta cgctggtcgc cctcgtagct gagcgtgccg aagtgacccc
      841 agtccgcctc gaaacgttca ccggcctcag gctccatccg cacgaaggcg cgcttgggcc
      901 ttggctgcgg gcgcacgttg tggatgtagt cacgcaggat ggtgtggcca ccgtcatagc
      961 cgaggggtcg caatcgttgc tcgatgacgg ctgcgctggc ccgggcgtcc ttctccagca
     1021 gttcggcgat ggtgggtttg aatggatcca gcttgctggt gcgggatcgc gttgccggcg
     1081 tctgcgccgg cgcctccaaa tacttcttga tggtccgcca actcatgcgc agatgcagtt
     1141 cgatcttgcg aatgggccag cgctccgtcc aatacaggtg atgcaaatcg ttgatcgcgt
     1201 cggtggtgag catggctcgc ttccctttat ggggcagaag gcgacccttt gaccagaggg
     1261 ctgttgttgg caagaaagat ccagcacttt tgattcaatg acttgcggga tagctgggct
     1321 gttgttggca agacttggag ctgagttgtt gttggcaaga cggcgttgtc gccgtcgctc
     1381 gtccctttgc cgctggcgcc gccggttgcg ttccgcccgc gcaggattgt gctcaagcaa
     1441 ctgcgtccag tagtccgggt gctccgcgcg ccattgctgc gcgctctcca gacacccctg
     1501 tcgatacacc ggatcggtgg cgaccttccg ccgatggtaa tcgttacggc gccgccgctg
     1561 gcaagccgga tcgctgcaag cggcctgagc aggctggaac ttggatggct gaaacgaacg
     1621 caggcaatag cggcagaccc gttctcccat gagcccgttc tcgcatgccg cagcgctccc
     1681 gtgcagacgg cgcgttcagg caagcaattt agaaactgat ttagagtgtg taccgcgtcc
     1741 ccgccaatgg gtctgttctt ttgaactaca aaaccggcgg aaatgctgct gttttattcc
     1801 ggcgctgaca tccgccatct ttgagtctgg gacttttatg ccagcggggt aactttggcc
     1861 gtcgatctcg gcatgaacct tcaacccttt cttcgtcgtc gttccggcaa tgaggttgac
     1921 gatcaccttc agactggtga gaggttgtcc gcgccagttc ttactgatga aggagaacag
     1981 gcggtgttcg atcttgttcc acttgctcgt gccaggcgga aa
//
LOCUS       DATMGX010000239         6144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_271626, whole genome shotgun sequence.
ACCESSION   DATMGX010000239 DATMGX010000000
VERSION     DATMGX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271626"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /gene="pgeF"
                     /locus_tag="VNR65_03880"
     CDS             <1..558
                     /gene="pgeF"
                     /locus_tag="VNR65_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWJ17849.1"
                     /translation="VHSARAVIAHKPWEASDAPQADAIVTNTPNLAVSVLTADCVPIL
                     MADAAAGVVAAVHAGWKGAKAGIIESAVEAMETLGASGSRIAAAIGPAISEAAYEVGP
                     EFKAAFIAGCTGNERYFSEARLGHIHFNLPQYVMDKLKILGIAHVNDAGVCTYGNESI
                     FFSYRRSFHRREPDYGRQISAIVFR"
     gene            752..1867
                     /locus_tag="VNR65_03885"
     CDS             752..1867
                     /locus_tag="VNR65_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17850.1"
                     /translation="MTRENKPAAHSGAGRAASLLTGALLLAGLTGCSGDLFQKGDSAS
                     SSIKAKPTVALAPAQGIPQKYASKVDEQLATSIKANGIAIVDAKDALYVVKPTYIAQS
                     QAKHGTKVAYTIDVMDKAGNKVRSITGEEIVSPKRGGDSWHHVTEEAAQKVALKSATD
                     VNAWIENPTAQASAPAIAEAKPAEAAPVKAAATSASVPVRSAANTPPKAPRAKAPVET
                     ASLASAVDTSAPAAPVQTAAVAPQEIVAVVPAVSGAPGDGKTALSDAMKKALGRQGVK
                     LTSAAGAGTYKIQGQVEMGSVSNGQQPITIRWVVIDPSGKQMEKTVVQNNKISAGSLD
                     GAWGEVAEQAAGAAASEVSKLLSKGSGQAQAASGSAG"
     gene            1999..2931
                     /locus_tag="VNR65_03890"
     CDS             1999..2931
                     /locus_tag="VNR65_03890"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWJ17851.1"
                     /translation="MKLVAGNSNRPLAHAIAAYLKTPLTQCHVRRFSDMEIFVEIREN
                     VRGEDVFIIQSTSYPANDNLMELLIIIDALRRASARRITAVVPYFGYARQDRKTLPRT
                     PISAKLVANMITHAGTDRVLTVDLHAGQIQGFFDIPTDNLYAVPVMVRDIRKRFTEGN
                     ITVVSPDVGGVVRARGLASRIDAPLAIIDKRRDRPGESEVMNVIGNVSGQHCIMIDDI
                     IDSGGTLCNAAGALLEKGARSVAAYITHGVFSGGAVAKIMASKIETLVITDTILPTEA
                     VRVASNIEVVSLAGLIGEAILRTSGEESVSSLFD"
     gene            3021..4159
                     /locus_tag="VNR65_03895"
     CDS             join(3021..3453,3453..4159)
                     /locus_tag="VNR65_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611739.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWJ17852.1"
                     /translation="MANKKFFVELDAAERARLTALISKGKSSAKSLLKARILLKADQG
                     EGGEAWPDEKICEALDTNVSMVTRVRARFVDQGLDAVLTRKKRETPPIQPIFDGEAQA
                     KLTALACSAPPAGHARWTIRLLSEHVVERKIVPAAHFNTVGRGVKKNELKPHLSEYWV
                     IPPKANAAFVAAMENVLEVYTRPHDPKRPLVCLDETSKQLVAETRAPLPMKPEHPARF
                     DYEYKRNGTANIFMLFAPLEGWRTAKITERRTAIDYAHVLKDLSDIHFAAAEKIVMTQ
                     DNLNTHAQASLYEAFEPAEARRLVERFEWIYTPKHGSWLNLAESELGVLAGQCLDRRI
                     PDRATLEREVAAWGSERNTHNVTADWHFTTADARVKLKSLYPAFF"
     gene            complement(4504..5673)
                     /locus_tag="VNR65_03900"
     CDS             complement(4504..5673)
                     /locus_tag="VNR65_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HWJ17853.1"
                     /translation="MNIGKTLFAQIMDFLPWSTFTRIVARYGGDQRVRTLSCAEHYRA
                     MAFAQLTYRESLRDIETCLSAQASKLYAMGFRDPVRRSTLADANEGRDWRIYAELAQR
                     LTGQARKLYLGEDLGLDLANTVYALDSTTIDLCLAVFPWAHFRTAKAAVKMHTLLDLR
                     GNIPSFIHISDGKLHDVHALDLLLPEAGAIYVMDRGYVDFGRLHGLHMAGAFFVTRAK
                     SNMDAHRVYSAPADRAAGIICDQTIALDRHYTAKSYSEHPRRTRFRDPETAKTLIFLT
                     NNFGLPAATICALYKSRWQVELFFKWIKQHLRIKRFYGTSENAVKTQIWIAVSVYVLV
                     AIVKKRLNLEASLYTLLQILSVTLFEKMPINQALVGGGPKYDNTQINNQLNLFAY"
     gene            5848..>6144
                     /locus_tag="VNR65_03905"
     CDS             5848..>6144
                     /locus_tag="VNR65_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17854.1"
                     /translation="MTQPGRIPDLGEQAYAGCMIDEGAIQYRWNTVGSKLDERGRRLF
                     AAAEVRTAGWGGLSVVARITGLARSTINRGEDDLDAEPLTKGKIRRSGGGRRAVA"
BASE COUNT         1300 a         1764 c         1861 g         1219 t
ORIGIN      
        1 gttcactccg cgagagctgt catcgcccat aagccttggg aggcgtcaga cgctccgcaa
       61 gccgacgcca ttgtgacaaa cacccccaat ctcgccgtaa gcgttctcac cgccgattgc
      121 gtgccgatcc tcatggccga cgctgcggcg ggtgtggtcg ccgctgtgca tgccggctgg
      181 aaaggtgcga aagcgggcat tatcgagagt gcagtcgagg caatggagac ccttggagca
      241 agcggatcgc gtattgcggc tgcgattggc cccgcaatct ccgaggcggc ttacgaggtc
      301 ggcccggagt tcaaggccgc attcatcgca ggttgtaccg ggaatgagag atatttttct
      361 gaggctcggc tcggtcacat tcatttcaat ttgcctcaat atgttatgga taagctcaag
      421 atccttggca tcgcacatgt caacgacgct ggtgtgtgca cctatgggaa cgaatcgatt
      481 tttttcagct ataggcgttc ctttcatcgg cgagagcccg attatggacg tcaaatttcc
      541 gcaatagtgt ttcgatgatg tggataacta ggcagacaac ccccgatttt agcgggtttt
      601 tgctagtcag gtcgcgtgtg tgtctcccgc gcctagacgg cgtttgcggc ggccgctaga
      661 tttcaatcgt taaaaagatt cggcggaata ataagtgccg gtatagtcaa gtaccgccgt
      721 cctggagcca tgaaaatcaa ggagtggaaa agtgacgcgc gagaacaaac cggccgcgca
      781 cagcggagct ggccgtgctg cgtcgctctt gactggggcg cttttgcttg cgggccttac
      841 cggctgcagc ggtgatctct ttcagaaggg cgattcggcc agttccagca taaaggccaa
      901 gcccacagtc gccctggcac ctgcacaggg gattccgcaa aaatatgcga gcaaggtgga
      961 cgagcagctt gccacatcga tcaaggcaaa cggtattgcg atcgtcgacg ccaaggacgc
     1021 cctctacgtc gtcaagccta cctatattgc tcagtcgcag gctaaacacg gcacgaaagt
     1081 ggcatacacg atcgatgtca tggacaaagc cgggaacaag gttcgctcca tcacgggcga
     1141 ggaaatcgtt tctcccaagc gcgggggcga ttcctggcac cacgtcactg aagaggccgc
     1201 tcagaaggtt gctctgaagt cggcaacgga cgtgaacgcc tggatcgaaa atccgaccgc
     1261 tcaggcttcc gccccggcaa tcgcggaagc caagcctgca gaggcagctc cggtcaaggc
     1321 agcagccacc tcggcttcag tgcctgttag gtccgcagcc aacactccac ctaaggcccc
     1381 gcgtgcgaag gctccggtag aaacggcgtc gcttgcatcc gcggttgata cttcggcgcc
     1441 cgcggctcct gtccagacag ctgcggttgc tcctcaagaa atcgtggccg tggttcctgc
     1501 tgtgagcggc gcgcctggtg atggcaagac tgctctttcc gatgccatga agaaggcact
     1561 cggtcgccag ggcgtcaagc ttacttcggc agcaggggct ggcacctata agatccaggg
     1621 gcaagttgag atgggctctg tctcgaacgg tcagcagcca attaccatcc gttgggtcgt
     1681 gatcgatccg tcaggcaagc agatggaaaa gaccgtcgtt cagaacaaca agatctccgc
     1741 cggcagcttg gatggcgcat ggggcgaggt tgcggagcaa gcggcgggcg cagcagcctc
     1801 ggaggtctcg aagctcctga gcaagggttc cggtcaggcc caagcggcat ctgggtcggc
     1861 aggctaatac aatcgtagcc tgcttcaacg ttgcgggtgg catttgcaca ctcgcaggtt
     1921 tacagataac gagcgcgctg ctagaagcgc gctcgttgcc gttctgtgaa cctattatga
     1981 caatggactg accctctcat gaagctggtc gctggcaata gcaataggcc cctggctcac
     2041 gccatcgcgg cctatctcaa gactcccttg acgcaatgcc atgtgcgccg cttttctgac
     2101 atggagattt tcgtcgagat ccgggaaaat gtccgcggag aggacgtttt tatcatccaa
     2161 tcgacgtcct atcccgccaa tgacaatctc atggagttgc tcatcattat tgacgcgctc
     2221 cggagagcct cggcgcggcg gatcactgcc gtagtcccct atttcggcta tgcgcgccag
     2281 gatcgcaaaa cgctcccgcg cacgcccatt tccgcgaagc tcgtcgcaaa tatgatcacg
     2341 catgccggca cggaccgggt gctgacggtc gatctgcacg ccggtcagat ccagggcttc
     2401 tttgacatcc cgaccgataa tctctacgcc gtgcccgtca tggtgcgcga catccgaaag
     2461 cgcttcacag agggaaacat caccgttgtt tcgcctgacg ttggcggcgt ggtgcgcgca
     2521 cgtggcctcg caagtcgcat cgacgctcct ctcgccatta tcgacaagcg gcgtgaccgg
     2581 cccggcgaat ccgaggtgat gaatgtgatc ggcaacgttt ccggccagca ttgcatcatg
     2641 atcgacgaca ttatcgattc tgggggcacg ctttgtaacg ctgcgggagc tctcctcgaa
     2701 aagggcgcgc gctccgtcgc ggcctatatt acgcatggcg ttttctccgg cggcgccgtg
     2761 gccaagatca tggcttccaa aatcgaaacg ctcgtcatta cggacacgat cctgccgacg
     2821 gaagcggtgc gggtggcaag caatattgag gtggtttcgc tcgcggggct gatcggcgag
     2881 gccattctgc gcacctcggg cgaggagtcg gtgtcgagcc tgtttgacta gtggcctgca
     2941 tcatctaaat tgaactattg cgttctgctg cgaacgctga ttcttgacca gtcaccttct
     3001 gagggcaagg ggactggtcc atggccaata agaaattctt cgtcgaactc gatgcagccg
     3061 agcgtgcacg cctgactgcg cttatttcga aaggcaagtc atctgcgaag tcgcttttga
     3121 aggcgcgcat cctgctcaag gccgaccagg gcgagggcgg cgaggcgtgg ccggacgaga
     3181 agatctgcga agcgctcgac accaacgtct caatggtcac gcgcgtacgc gcgaggttcg
     3241 tcgatcaggg cctcgacgcc gtgctgacgc gcaagaagcg cgaaacgccg ccgatccagc
     3301 cgatcttcga tggcgaggcc caagccaagc tgaccgcgct cgcctgttcc gcgccgcccg
     3361 ccgggcacgc gcgctggacg atccgtctgc tgtcggagca tgtggtcgaa cgcaagatcg
     3421 taccagccgc acacttcaac accgttggcc gggcgttaaa aaaaacgaac tcaaaccgca
     3481 cctgagcgaa tattgggtca tcccgccaaa ggccaacgcc gccttcgtcg ccgccatgga
     3541 gaacgtgctc gaagtctata cgcggccgca cgacccgaag cggcctctgg tctgcctcga
     3601 cgagacatcc aagcagcttg tcgccgagac gcgcgcgcca ttgcctatga agccggaaca
     3661 tccggcacgc ttcgactacg agtacaagcg caatggcacc gccaacatct tcatgctgtt
     3721 cgcgccgctc gaaggctggc gcactgcaaa gataactgag cggcgcacag ccattgacta
     3781 tgcgcatgtt cttaaggact tgagcgacat ccacttcgcc gcggcggaga agatcgtgat
     3841 gacgcaggac aatttgaata cgcacgctca ggcgtcgttg tacgaagcgt tcgaaccggc
     3901 cgaagcgcgg cgcctcgtcg agcgcttcga gtggatttac accccgaagc atggcagctg
     3961 gctcaatctt gcggaatcgg agttgggcgt gctcgccggc caatgtctcg accggcgtat
     4021 tccagaccgc gcaacactcg aacgcgaagt cgcggcctgg ggctcagagc gcaacaccca
     4081 caacgttacg gccgattggc acttcaccac cgccgacgcc cgtgtaaaac tcaaaagcct
     4141 gtaccccgca tttttttagg tgattcaggg cactaggcat acgccgccgc tctggcggtt
     4201 cgctgtctgc gacgccgcag ccagctcgcc gggtgactgg gctatggtcc attttgtttg
     4261 cggaatccgg cattttgctg aaccttttgc ccggcggatc gccctggcga atatggtagg
     4321 aaaatgcgcc ttaccggaag gtaaatggcg ataagacgtt gagtttaaag gtaaaaatct
     4381 ggagaaggga gcgcgccgcg ctgccgcgaa aggcgcttag gctgttaatc cgcgaagccg
     4441 aaacatattt aaccgacttt tgcgcgccca attaaggaaa taattccggc gactgctgtc
     4501 cagctaataa gcgaatagat tcaattggtt attgatttgc gtattatcgt atttgggacc
     4561 gcctccgaca agggcttgat ttatcggcat tttctcgaag agggtgaccg aaaggatctg
     4621 gagcaatgtg tagagggaag cttcgagatt gagccttttt ttgacgatgg cgacgaggac
     4681 gtagaccgag acggcgatcc agatttgggt ttttacggca ttttccgacg tgccgtagaa
     4741 ccttttgatg cgcagatgct gtttgatcca cttgaagaaa agctcgacct gccagcggct
     4801 cttgtagagg gcgcagatgg tcgccgccgg caggccgaaa ttgttggtga ggaagatgag
     4861 ggtcttggcg gtttcgggat cgcggaagcg tgtccggcgc ggatgctctg aatagctctt
     4921 ggcggtgtag tgacggtcga gtgcgatggt ctgatcgcag atgatgccgg ctgcgcggtc
     4981 agctggggcc gaatagaccc ggtgggcatc catattcgat ttggcgcgcg tgacgaagaa
     5041 cgcgccggcc atatgcaaac cgtgaagccg gccgaaatcg acgtagccgc gatccatgac
     5101 atagatggcg ccagcctcgg ggagcagcag atcgagtgcg tggacgtcgt ggagcttgcc
     5161 gtcggatata tggataaagc tcgggatatt tccgcgcaga tcaagcagcg tgtgcatctt
     5221 caccgccgcc ttggccgtgc ggaaatgcgc ccacggaaag accgccaggc aaagatcgat
     5281 ggtcgtcgaa tcgagcgcgt agacggtatt ggcgagatcg agccccaaat cctcgccgag
     5341 atacagcttg cgcgcctggc cggtcagccg ctgggccaac tccgcgtaga tccgccagtc
     5401 gcgcccctcg ttggcatcgg ccagcgtcga gcggcgcacc ggatcgcgaa agcccatggc
     5461 atagagcttc gacgcttgcg ccgaaaggca ggtctcgata tcgcgcagac tttcccggta
     5521 ggtcagctgg gcgaacgcca tggcccgata atgctcggcg caggacagtg tccgcacccg
     5581 ctggtctccg ccataccgcg cgacaatccg cgtgaaggtg ctccacggca gaaaatccat
     5641 gatctgagcg aaaagcgtct tgccaatgtt caccggcgcc tcctcggata tcttccgaga
     5701 gatgccagat cgcgttcaaa tcgacaccac gcaaaagcgc cagaaaacca cagaaaatca
     5761 caatgctatg ggcaggcagc cgtcaatcag ccggacagat gtgatcccga acaataactg
     5821 aatcacgctc tatgggcgtg cgcctgagtg actcagcctg gcaggattcc ggatctcggc
     5881 gaacaggcgt atgccggttg catgatcgac gagggcgcca ttcaatatcg ctggaacacg
     5941 gtcggcagca agctcgacga gcgaggccgg agattgttcg cagctgccga ggtgcggacc
     6001 gcgggatggg gcggtctctc ggttgtggcg cggatcacgg ggctcgcccg ctcgacgatc
     6061 aatcgcggtg aggacgatct cgacgcggag ccgctgacga agggcaagat tcgccgctct
     6121 ggtggcgggc gccgggccgt tgcc
//
LOCUS       DATMGX010000240         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_6188,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000240 DATMGX010000000
VERSION     DATMGX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6188"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            682..1845
                     /locus_tag="VNR65_03910"
     CDS             682..1845
                     /locus_tag="VNR65_03910"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ17855.1"
                     /translation="MSQPNDLSRSLVPFEQDATLVAVIELSQSSWLIAGVVPGIERQP
                     LKKMAPDEAALLRLLHRWRDEAIQNGRSITRIAVALEAGRDGFWLARWLTKHGIEAHV
                     IHSSSVAVSREHRRAKTDRLDTAMLSRVFLGWLRGERGHCATVAVPTIEEEDAKRPSR
                     ERENLVGERTRVINRLKAALTRLGIRGFNPKLRKALQRLDSLHTPEGFPIPPNTLDEM
                     RRDLARLAILREQIKAIEQARLERLQQAPEAGPNAMIRLLASILGVGIETADMLVHEV
                     LSRNLRDRRAVGRFVGLTGAPDESGARRREKGLTKAGNARVRRGLIQLAWRFLLFQKD
                     SALAKWFRSRTEGPSGARKTKMIVALARKLIIALWRMVNTGEVPHGVVLRQPA"
BASE COUNT          479 a          666 c          690 g          435 t
ORIGIN      
        1 gggggggcca cgaaagtgca cacctgcgac cgccgcgcgt ctgcggcggt tttggcaaat
       61 gagcaaacgc cctgcaccgt cgggtgggcg acgccgttcc atcgtgcttg ccccatgtgc
      121 tggtcgccgt tcggcggcag cacatcaagt cgtcacgggg ctgatgttgc tataaggatt
      181 ccgaccagtg gtggaaggcg attactcttt tagatggcta ttctcaacat taaatctact
      241 tcgatggaag agccgctcga cgggtgatca cgccctgcaa aattgccgag acctcactag
      301 cagataccga tactaggtat ccgttattcg gtccatcgac caactggtaa cccgacatgc
      361 aagcattatg tgacggttgc caatattcat atagagggtc tataaatagt atccggtcct
      421 gttgctctgt aaaattgtag acgactgcta catgtgaaat tggaccgcac ctaacgatca
      481 cggtatcatc gcgcaaaata ttagatagga ccgattttcg aggcacattc aatacatagg
      541 gatcgctctc gttcaccccc gccggaacca ggagataaag acgacctaaa cctgcttcat
      601 ttgtcattat cgttagaagc tatggggcgg ccggcgagga acaggcatca ttgctgcaat
      661 gatcaccgcg tggagtgtcc gatgtcccag cccaacgatc tgagccggtc cctcgtcccc
      721 ttcgagcaag acgccactct tgttgccgtc atcgagctca gccagtcaag ctggctgatt
      781 gccggagtcg ttccgggcat cgagcggcag ccgctgaaga agatggcgcc ggacgaagcg
      841 gcgctgctgc ggctgctgca ccgctggcgt gacgaggcga tccagaacgg tcgctccatc
      901 acacgcatcg ccgttgccct tgaggctggg cgtgacgggt tctggctggc acggtggttg
      961 acgaagcatg gcatcgaggc tcatgtcatc cattccagca gcgtcgcggt ctcacgcgag
     1021 cacagacggg ccaagacgga tcgcctcgac accgcaatgc taagccgggt gttcctcggc
     1081 tggctgcgcg gcgaacgcgg ccactgcgca acggtcgcgg ttccgacgat cgaggaagag
     1141 gacgccaagc ggccaagccg tgagcgggag aacctggttg gcgaacggac acgcgtcatc
     1201 aatcgcctga aggcagcgct gacgcggctc ggcatccgtg gcttcaaccc gaaactgcgg
     1261 aaggcgctgc agcgtctgga ttcgctgcac acgcccgaag ggtttccgat cccgccaaac
     1321 acgctcgatg agatgcggcg cgacctcgcc cgactcgcga tcttacgtga gcagatcaag
     1381 gctattgaac aagcgcgttt ggagcgactg cagcaggcgc cggaagcagg accgaatgcg
     1441 atgatccgac tgctagccag cattcttgga gtcggcattg aaacggcgga catgctggtg
     1501 cacgaagttc tctcgcgcaa cctgcgcgac cgcagggcgg tcggacgttt cgtggggctc
     1561 accggtgcgc cagatgaaag cggggccaga cgacgcgaga agggtctcac caaagcgggc
     1621 aatgcccgcg tgcgccgcgg cctgatccaa ctagcttggc gcttcctctt gtttcagaaa
     1681 gacagcgcgc tggccaagtg gttccgaagc aggacggagg gacccagcgg cgcacgcaaa
     1741 acgaagatga ttgtcgctct tgcccgcaag ctgatcatcg cgctgtggcg catggtcaac
     1801 accggcgagg tgccgcacgg cgtggtgctg cggcagccgg cctgaccaac actgtaatgt
     1861 agcacagcaa cacacggttt tcggcgcgtc tggctaagcg aacttgctca gccgccgagt
     1921 acgacccgag gtggtggtga cccgatgttg gacatggcca ttgaaccgtg gctcagaatg
     1981 ggcccgccac cccggagcct cgctgccgaa gcgcatgtct gcttcatggt ccgattccgg
     2041 atgggatcaa ccgaatacaa gtttgtggcg cgagcttgcg cgccccaagg cgggctcccc
     2101 tcggttcgtt tgctttgtcc aacagcgaca tttggaacca aaccatcgtc gttgaagtgc
     2161 tgacaagccg catggtgcgg cgctgctaat acgttacgta acggtgaaca gtgcatccca
     2221 cgatgccgcc agccaggaca agccgcctga tgctccaatt gtattgcgtt
//
LOCUS       DATMGX010000241         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_356748, whole genome shotgun sequence.
ACCESSION   DATMGX010000241 DATMGX010000000
VERSION     DATMGX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356748"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VNR65_03915"
     CDS             <1..862
                     /locus_tag="VNR65_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWJ17856.1"
                     /translation="PRDTVLVPTPSYPLFDFLADLHDVKIEPYELVYDHGWQMDFHSA
                     QAAIERARRAGSRCRAVLAVHPNNPTGSFVKPPEAEELNRICAANEMAIVADEVFLDY
                     SLTKGPPLTFSSNSTVLTFTLSGLSKISGLPQMKIAWIAANGPQPLLSDAMNRLEVIA
                     DTYLSMNAPLQWAVPVMLEERHGIQRQLLQRMVANLGLLDSQLASQELCRRLQVEGGW
                     YVVLRVPVAGPDEDLAIALLRETGVLVQPGHFYDFTSEGYLVISLITPGDEFAEGIRR
                     LLAFIAELHS"
     gene            1039..2016
                     /locus_tag="VNR65_03920"
     CDS             1039..2016
                     /locus_tag="VNR65_03920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWJ17857.1"
                     /translation="MPKYSIVVPFHNEEENVTELYDRLKVVMEATGEPFELVFVDDGS
                     RDHTFWMLQQIAAVDRRVVVVKLRRNFGQTSALAAGFDHAEGEYVIAMDGDLQHDPHD
                     IPLFLQKLREGYDIVSGWRKQRIDNFVMRRIPSKIANWLMAKLSGVDLHDFGTTFKAY
                     RREVVQEIPLYGELHRFIPALASAYGATICEIPIRNVNRERGESHYGISRTFRVFFDL
                     ITIRFLLKYMARPLHFFGTVGALGSLAGFVIALWMLFDKFVRGLNVMDEHGPLLVFAA
                     VLIVAGVQLIAVGLLGELQVRHFHEPTKRAPYTVDRVLRSSHSQSALAD"
BASE COUNT          414 a          588 c          620 g          462 t
ORIGIN      
        1 tccgcgagac acggtgctgg ttcccacccc gagctacccg cttttcgatt tcctcgccga
       61 tctccacgac gtgaagatcg aaccgtacga gctggtgtat gaccacggat ggcagatgga
      121 ttttcactcg gcgcaggccg cgatcgaacg cgcgcgccgc gcaggttcgc gttgccgggc
      181 cgtgctggcc gtgcacccca ataacccgac gggttcgttc gtgaagccgc cagaggctga
      241 ggaattgaac cgcatttgtg cggccaacga aatggccatc gtcgccgatg aagtgttcct
      301 cgattactcg ctcacgaagg gtccgccact tacgttttct tcgaacagca cagttctgac
      361 cttcacgctc agcggactgt cgaaaatttc cggtctgccg cagatgaaaa tcgcatggat
      421 tgcggcgaat ggcccgcagc cgctgctgtc ggatgcgatg aaccggctcg aggtcatcgc
      481 cgatacctac ctatccatga atgcgccatt gcagtgggct gttcctgtca tgctggagga
      541 gcggcacggc atacagcgcc aactgctgca acgcatggtt gcgaatctcg gtctgcttga
      601 ttcgcaactc gcctcgcaag aactctgtcg ccgactgcaa gtcgagggcg gatggtatgt
      661 cgtgttacgg gtcccggtgg cgggccccga cgaggatctt gccatcgcgt tgctgcgcga
      721 gacgggggtg ctggtgcagc ccggccattt ttatgacttc acttcggaag gatatttggt
      781 gatcagccta atcactcccg gcgacgagtt cgcggaagga atccgacgct tgctggcatt
      841 tattgctgag cttcattcgt aaaaacgctc tgcctcgggg aaagcccgat tgtcctgagg
      901 acaagaaagt gccagataaa cgcatggtgc tagcacaagt gcaacgactt gcagcccggg
      961 tgtttcgcgt tggcggaaga gtaattccag taaaacatcc gtccattttg ggatgacgga
     1021 cgttataatc ataaaaccgt gcccaagtac tcgattgtgg tcccatttca taacgaagaa
     1081 gagaacgtaa ccgagctcta tgatcggctc aaggtggtga tggaagccac gggcgaaccg
     1141 ttcgagctgg tattcgtgga cgatggcagc cgcgatcata cgttctggat gttgcagcag
     1201 atcgcagcgg tggaccgccg cgtggttgtg gtgaaactgc gccgcaactt cgggcaaacc
     1261 tcggcgctgg ccgcgggctt cgatcacgcc gaaggcgagt atgtgattgc catggacggc
     1321 gatctgcagc acgatccgca tgacattccg ctgtttctgc aaaagctgcg cgagggctac
     1381 gacatcgtca gcggatggcg caagcagcgc attgacaact tcgtgatgcg gcgtattccc
     1441 tccaagatcg cgaactggct gatggccaag ttgagcgggg tcgatttgca cgatttcggt
     1501 accaccttca aggcttatcg tcgcgaggtg gtgcaggaaa ttccgctcta cggggaattg
     1561 catcgcttca ttccggcgct ggcgtcggct tatggcgcca ccatttgcga gatccccatc
     1621 cgcaatgtca accgcgaacg cggcgagtcg cattacggca tctcgcgcac cttccgcgtg
     1681 ttcttcgatc tcatcaccat ccgttttctg ctgaagtaca tggcccggcc gctgcacttt
     1741 ttcggtaccg tcggggcgct aggttcactg gctggcttcg ttatcgcctt gtggatgctc
     1801 ttcgacaagt tcgttcgcgg tctcaacgta atggatgagc acgggccgct cctggtgttc
     1861 gccgcggtgc taatcgtggc aggtgtgcaa ctgatcgccg taggactgct gggcgagttg
     1921 caggtgcggc acttccacga gccgacgaag cgggcaccgt ataccgtgga tcgcgtattg
     1981 cgctcatcgc acagccaatc cgctctggcc gactaaacaa ggggataggg actgggggct
     2041 agtggctagt aaaacctgat gttcactaac ccctagcccc taat
//
LOCUS       DATMGX010000242         3416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_290095, whole genome shotgun sequence.
ACCESSION   DATMGX010000242 DATMGX010000000
VERSION     DATMGX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290095"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VNR65_03925"
     CDS             complement(<1..206)
                     /locus_tag="VNR65_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17858.1"
                     /translation="MDTFVENSGVAEQEKQIHVVPTENETHIQVPIEKAETAGAPLDD
                     TAELNSQGEEPPQDIKIVHAEKPDP"
     gene            471..1877
                     /gene="argH"
                     /locus_tag="VNR65_03930"
     CDS             471..1877
                     /gene="argH"
                     /locus_tag="VNR65_03930"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWJ17859.1"
                     /translation="MTRMANKMWGGRFEAKPAEIMEEINASISFDKRFYAEDIRASQA
                     HAKMLGACDIISAKEAEDIIAGLDAVREEIELGRFEFRREYEDIHMAVEARLKELIGF
                     VAGKLHTARSRNDQVATDFRLYIRGAIDHLDGQLLSLQRALARKAAAHADTIMPGFTH
                     MQTAQPVSFGHHCLAYVEMLARDRGRFADARKRVNESPLGAAALAGTSFPIDRAMTAA
                     ELGFARPMANSLDAVSSRDFVLETLAAAAISASHLSRLAEEIVIWFSAGFGFISLSDR
                     FTTGSSIMPQKRNPDAAELIRAKAGRISGAFVSLLMVMKGLPLAYSKDMQEDKEAAFL
                     AIDALSLGLAAMTGMVEDMEPNPEKMRAAAAQGFSTATDLADWLVRCAGLPFREAHHV
                     TGRIVKAAENAGLDLAHVPLDTMKKIEPRIDASVFEVLKIDKSVESRRSFGGTAPQNV
                     RAAAEIWLKSLELENASG"
     gene            2118..3386
                     /gene="lysA"
                     /locus_tag="VNR65_03935"
     CDS             2118..3386
                     /gene="lysA"
                     /locus_tag="VNR65_03935"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWJ17860.1"
                     /translation="MHYFSYRNGILHAEEVALDAIAREVGTPFYCYSAATIERHYNVF
                     SEAFAGRDTLVCYSIKANSNISVIRTLARLGSGADVVSEGEIHRALAAGVPPHKIVFS
                     GVGKTRAEMAFALDTGIFGFNVESEAELRALSEVASARSVSARIAFRINPDVDAKTHA
                     KISTGKSEHKFGVSWKDARALYALARSLPGINVQGVHMHIGSQITHMEPFKNAFALLA
                     SLVEDLRSDGHTISFVNLGGGLGIPYRWDLQEPPLPNDYAEIVTALANRLGAKLLFEP
                     GRVIAGNAGILVTEVIYTKRGGAKHFIIVDAGMNDLIRPTLYDAYHDIWPVREALRNG
                     NMARSDVVGPVCETGDFIAKDRAMPETAAGDLLAILTAGAYGAVQASTYNTRRLVPEV
                     LVSGANYAVIRPRPSYEDMLKAEAIAPWLR"
BASE COUNT          643 a         1054 c         1025 g          694 t
ORIGIN      
        1 ggatccggtt tctccgcgtg aacgatttta atgtcttgcg gcggctcctc gccttggcta
       61 ttcaactccg ccgtgtcatc gagaggcgcg cctgccgttt cggccttctc aatggggacc
      121 tgaatgtgcg tttcattctc cgtggggacg acgtgaattt gtttctcctg ctcagcgacc
      181 ccgctgtttt cgacgaaggt gtccataagc ttctccttga cggatgacca gacgagcctg
      241 gcgtacaacc tgccctttcg tactgaatgc gctgcagttt cagacgttcc ccggggcgcg
      301 cagcaaagac cccgcagaag catcctaagc ctgcaaattg tttacttggg gggcgcgggc
      361 cgccaccgca agcaggctca agcgtgacgc tctcatacat caggctaatg aggccgctct
      421 tgtgcagtgc gaaacagcgc gctaatgctt cgctttaagc ccatcaggcg atgacaagaa
      481 tggccaataa aatgtggggc ggacgcttcg aagcgaagcc cgccgagatc atggaggaga
      541 tcaacgcctc catttcgttc gacaagcgct tctatgccga ggacattcgc gccagccaag
      601 cgcacgcgaa aatgctgggc gcatgcgaca ttatttccgc gaaggaagca gaagacatca
      661 tagcgggtct cgatgccgtc cgcgaggaga tagagcttgg ccgcttcgag ttccggcggg
      721 agtacgaaga catccacatg gctgttgaag cgcgcctgaa ggagctgatt ggcttcgtcg
      781 ccgggaagct tcatacggcg cggtcgcgca acgatcaggt cgccaccgat ttccgccttt
      841 atatccgggg cgcgatcgat cacctcgacg gccagcttct gagcctgcag agggcgcttg
      901 cccgcaaggc cgccgcgcat gcggacacca tcatgccggg cttcacccac atgcagacgg
      961 cgcagcccgt cagtttcggg catcattgcc tcgcttatgt cgagatgctg gcgcgcgacc
     1021 gcggccgctt cgccgatgcg agaaagcgcg tcaatgagtc gccgctggga gccgcggcgc
     1081 tcgcggggac ctcctttccc atcgatcggg ccatgacggc ggcggagctt ggtttcgccc
     1141 gtccgatggc gaattcgctc gacgccgtat cgtcacgcga cttcgtgctc gaaacgctgg
     1201 ccgcggcggc gatctccgcc tctcatctgt ctcgcctcgc cgaagagatc gtcatctggt
     1261 tcagcgcggg cttcggcttc atctcgttga gcgaccgctt cacgaccggt tcctccatca
     1321 tgccccagaa gcgcaatccg gacgccgccg agctgatccg ggccaaggcc ggccggattt
     1381 cgggagcttt cgtctcactt ctgatggtca tgaaggggct tccgctcgcc tattcgaagg
     1441 atatgcagga ggacaaggag gccgcgttcc tcgccatcga cgcgctctcc ctcgggctcg
     1501 ccgccatgac aggcatggtg gaagacatgg agccgaaccc ggagaaaatg cgtgcggcgg
     1561 cggcgcaagg cttctcgaca gcaaccgatc ttgcggattg gctcgtccgt tgtgctggcc
     1621 ttcctttccg cgaggcccat cacgtcacgg ggcgcatcgt gaaagccgcc gagaacgccg
     1681 gccttgatct cgcccacgtt ccgctggaca ccatgaagaa gatcgaaccg cgcattgacg
     1741 cctcggtttt cgaagtgctg aagatcgaca agtccgtcga gagcaggcga agcttcggag
     1801 gcacggcgcc acaaaatgtg cgggcggccg cggaaatctg gttgaagtct ctggagttgg
     1861 aaaacgcttc agggtaaaga cagtttgggc tggcgcgccg gcggttgcgc cattacggga
     1921 ataagatgac tcgcacaatg cgcaatttgg ttctggctgc gggtatcgcg ctcgcgcttg
     1981 ccgcatgcgg gaagaaaagc gacttgcaac gtccgctacc gcctccagat gttccgaagg
     2041 atgcatcagg gaagaatgcg gatcccaacc gccccttcat cctggatccg cttctgcgtt
     2101 aaacgggcct ctgctcgatg cattattttt cctatcggaa cggcatcttg catgccgaag
     2161 aagttgctct tgacgcgatt gcgcgcgagg tcggcacgcc cttctattgc tattcggcgg
     2221 ctaccattga gcggcattac aacgtgtttt cggaggcctt tgccgggcgc gacacgcttg
     2281 tctgctactc gatcaaggcg aattccaata tctcggtcat tcgcacgctg gcgaggcttg
     2341 gctccggcgc tgacgtggtc tctgagggcg agattcatcg cgcgctggcg gctggcgtgc
     2401 caccgcataa gatcgtcttc tcgggtgtcg gcaaaacgcg ggccgaaatg gctttcgcgc
     2461 tcgacaccgg aattttcgga ttcaacgtcg agtccgaggc tgagcttagg gccttgagcg
     2521 aggttgcaag cgcccgcagc gtttcggccc gcatcgcctt tcgcatcaat cccgacgtcg
     2581 acgcgaagac gcatgccaag atttccacgg gcaagtccga gcacaaattc ggcgtgagtt
     2641 ggaaggacgc gcgcgccctc tacgcccttg cgcgctcgct gccgggcatc aacgtgcaag
     2701 gcgtccacat gcacatcggc agccagatca cgcatatgga gccattcaag aacgctttcg
     2761 ccttgctcgc gagtctggtg gaggacttac ggtcggacgg ccacaccata tccttcgtca
     2821 acctcggcgg cggcctcggc attccctatc gctgggacct tcaggaaccg cctctgccga
     2881 acgactatgc cgagatcgtg accgctcttg cgaaccgtct cggcgccaaa ctccttttcg
     2941 agccgggccg cgtcatcgcg ggtaacgcgg gaatccttgt caccgaggtg atctacacca
     3001 aacggggcgg cgcgaagcat ttcatcatcg ttgatgcggg catgaacgat cttatccgcc
     3061 caactctcta tgacgcgtat catgatatct ggcccgttcg cgaggccctt cgcaacggga
     3121 acatggctcg ctccgacgtg gtcggccctg tttgcgaaac cggcgatttt atcgcgaaag
     3181 accgcgccat gccggagacc gccgcgggcg atcttctcgc catcctgacg gccggagcat
     3241 acggcgcagt gcaagcgtcg acctataaca cgcgccgcct cgtgcccgag gttctcgtct
     3301 cgggcgcgaa ctacgctgtg atcaggccaa ggccctctta cgaagacatg ctgaaggccg
     3361 aagctattgc gccgtggctg cgctgatagg gcatgaagcc tcatcaggta agctgg
//
LOCUS       DATMGX010000243         3947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_172957, whole genome shotgun sequence.
ACCESSION   DATMGX010000243 DATMGX010000000
VERSION     DATMGX010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172957"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNR65_03940"
     CDS             <1..210
                     /locus_tag="VNR65_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17861.1"
                     /translation="DMEELHIKLRQPVTSALLADWRRLPIWRLLMRLPWTMGYDNIVR
                     IDLQPFAPPPDEIIRTMQRMWRYYR"
     gene            complement(201..1100)
                     /locus_tag="VNR65_03945"
     CDS             complement(201..1100)
                     /locus_tag="VNR65_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HWJ17862.1"
                     /translation="MVQHQESSALDRSVSLDDEEIPEAERGLTDKEQSEVREAQRPRA
                     PVVYEIIRLEGESELKRPIASLWWSGVAAGISIGFSFLSQAALAAGLPQSQWNHILTS
                     LGYSVGFIIVILGRQQLFTENVLTAVLPVITRWKVSWFLRMLRLWGVVLAANVLGCFV
                     FACAFALLPMLSDQVTGEMAKLVETLMRNSAWEMFAKGIGAGWLIASLVWILASVSSG
                     QFLVIGLLTYLISLLQFTHIVAGTAEVLYGWLAGFLTFYDASVKFFLPTLAGNVFGGT
                     ILFSVLSYGQVREEIFEERTDYR"
     gene            complement(1447..1695)
                     /locus_tag="VNR65_03950"
     CDS             complement(1447..1695)
                     /locus_tag="VNR65_03950"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HWJ17863.1"
                     /translation="MPKETKGQKDTVGRVMHEYKHGELETGTGTPVKSRRQAVAIALH
                     EAGASNQESPEKNRDNLKKTKRKEREGKTAKQQKEGHL"
     gene            2438..3244
                     /locus_tag="VNR65_03955"
     CDS             2438..3244
                     /locus_tag="VNR65_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HWJ17864.1"
                     /translation="MPTQEITIRPVQELTVRALLLGEGLDVKGLERQDAFSTNPLAFK
                     TASNGTVMLFKFGAVVFFNMSPIEEDDMVHGLTRRVIQPLKEQEVETAVVAIGPEEEP
                     LLSSSGAIRIRSAEPEKLLLIGEALAVSVALAYDERRIAAAFEKIEPIAASLIKRRLP
                     SGPRSGMLEQIGEALLIQKRLASRVDLDEKPDVLWDHPELERLWAKLVDEYDLMARAR
                     GVERKLVVIRETADTITDLISTRTSHQLEIYIIILIGIELVLALYDRFLK"
     gene            complement(3237..>3947)
                     /locus_tag="VNR65_03960"
     CDS             complement(3237..>3947)
                     /locus_tag="VNR65_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HWJ17865.1"
                     /translation="GVVLLPTLSRHLADQDEAGALTSQNRSLEFALLLTLPAAVALIV
                     IPKPIVQVLFQRGSFSPIDTVQVSAALAAFACGLPAFVMTKVFLPGFFAREDTKTPMW
                     FAMASVAVNIGGSLALFFVIGHVGIAIATSLSGWTNAILLSTTLMRRGHFQIDAALRR
                     RAPLILLASVLMGAALYYVNRVVAPYFEPGHSGTVQTIALLVLVGAGAFVYAAAAQIT
                     GAMRYSMIRRALSGARAT"
BASE COUNT          891 a         1160 c         1124 g          772 t
ORIGIN      
        1 gacatggaag agcttcacat caagctgagg cagccggtca ccagcgcgct tttggccgat
       61 tggcgccgcc ttcccatctg gcggctcctc atgcgcctgc cttggacgat gggctacgac
      121 aacatcgtgc gaatcgacct ccagcccttc gccccgcctc ctgacgagat catccgcacc
      181 atgcagcgta tgtggcgcta ttatcggtaa tcggtccgct cctcgaagat ctcctcccgc
      241 acctggccat agctcaacac ggaaaagagg attgttcccc cgaacacgtt tccggccagc
      301 gtggggagga agaacttcac cgatgcgtcg tagaacgtaa gaaaccccgc aagccagccg
      361 tacaacacct cagccgttcc cgcgacgata tgggtgaact ggaggaggga gatgagataa
      421 gtcaaaaggc cgatgaccag gaactggccg ctggaaaccg atgctaagat ccaaaccaag
      481 gaggcgatga gccagccggc gccgatgccc ttcgcaaaca tctcccacgc agaattgcgc
      541 atcaaagtct cgacgagctt cgccatctcc ccggtgactt ggtcactcag catcgggagg
      601 agcgcaaagg cgcaagcgaa gacgaagcag cccagcacat tcgcggcaag cacgaccccc
      661 caaagccgca gcattctcag gaaccagctc accttccagc gggtaatgac gggaagcaca
      721 gccgtcagca cattttccgt aaagagctgc tgccgcccga gaatcacaat gatgaagccg
      781 accgaatagc cgagcgaggt caggatgtgg ttccactgtg attgcggaag accggcggcc
      841 agagcagcct ggctgagaaa agaaaagccg atggaaatgc ctgccgctac gcccgaccac
      901 cacagcgaag caatcggccg cttcagttcg ctttcgcctt ccagccgaat gatctcgtag
      961 accacaggcg cgcgcggacg ttgggcctcg cggacctcgc tttgctcttt atcggtaagc
     1021 ccgcgctcgg cttcggggat ctcttcatcg tcgagcgaga cggagcgatc aagggcggag
     1081 gactcttggt gctggaccac tcgcaaaatc ctttggctga gagagcatca actgcaaaac
     1141 tccaaggctc agcactcgat ccatctctgt aagcaagcag aatctcagcc ttttccttat
     1201 tcccgaaaag gaacaggagc gcatggccat cgttgaccta caaaaaaggt ggtcgcgcct
     1261 tctaaaagcg agctcgcggt cgcacttacc ggcttcgacg ccacgatgcg tggggacgag
     1321 agcgccgtgc atggactctc gaacaaggtc ataagcgcga cgtcagagct tatcccgcca
     1381 gggttcgccg cgcaggttca caggccggcg gcgccgttgc accgttaaac cgaagatgga
     1441 ggaaggctac aaatggcctt ccttctgttg cttagcggtc tttccctcgc gctcttttct
     1501 cttcgttttc tttaggttgt ctcggttttt ctccgggctc tcctgattgg aagccccggc
     1561 ttcgtgcaag gcaatcgcaa ccgcctgacg gcggcttttt acgggcgttc cagtgccggt
     1621 ttcaagctcg ccatgcttat actcatgcat gacgcggccc acggtgtcct tctgcccctt
     1681 ggtttccttc ggcatattac tgtcctcgta aggctgacgc ctcagattgg ctgaggcgtc
     1741 ataagcaacc tcacttttga atgattgaac cctgacgagc ccttttctct tcactggccc
     1801 gagtgagagg tgtctgctgg tgctcacttc gccctttcat cgtccggaag aagctcacaa
     1861 tcgcatagac aatcacgatt ccgaacgtca tgaacgcgag tgccggaatg acttggtcga
     1921 tgctgagcaa tgggtggtcc tccatgttgc tgttgaagca ggcttgttag ccgtagccat
     1981 tcgaggagaa cccttggaaa atcgcaatga tccggttcga taaagcggat gttattccgg
     2041 attggaaaat aaatcgggca aacaattcag ttatgatcaa aagcgtagat cgctttacta
     2101 gagacttatt tagtctttat gtgtcgccat aagtcacgac gccgttgcca atggccatcc
     2161 ttggtggcca gtagagttac ttacttatat gatctcggcg cgcccgctgc gccatcatca
     2221 ggatcgccgc cgcggcgaga atatcgcgcc tgcgaaaatc cataagtttc cacccaaatg
     2281 ttcaccgcaa caaaacagtg ccagtgttag gtttcgggcg gcgcgcgttt ggggttagat
     2341 tccacgcgca tgtataatag aaagtgtctc cgacccggct cacagggatc ccaattgaca
     2401 gcgccgcaac agccgttagc aaaccatgag tgattgcatg ccgacgcagg aaattaccat
     2461 tcggcccgtt caggaattga cagtaagggc gctcttgctt ggcgaaggtc tcgatgtgaa
     2521 aggtctggaa cgccaggacg cgttctccac caacccgctc gcgtttaaga ccgcaagcaa
     2581 cggaacggtg atgctgttca aattcggcgc tgtcgtcttc ttcaatatgt cgccgatcga
     2641 agaggatgac atggtgcatg gcctgacgcg ccgtgtcatt caacccctga aagaacagga
     2701 ggttgaaacc gccgtcgtcg ccattggtcc ggaggaagag ccacttctct caagctcggg
     2761 cgcaatccgt attcgaagcg cagagcccga gaagctgctg ctcatcggcg aggcgctggc
     2821 ggtctcggtt gcgctggcct atgacgagcg tcgcatagcg gccgcttttg agaaaatcga
     2881 acccattgcg gcaagcctca tcaaacgccg cctgccgtca ggccccagat cgggcatgct
     2941 ggagcagatc ggggaggcgc ttctgatcca gaagcggctc gcgtcccggg tcgatctcga
     3001 cgaaaagccg gatgtcttgt gggatcatcc cgagttggag cgcctctggg cgaagctcgt
     3061 tgatgagtac gacctcatgg cgcgcgcacg gggtgtggag cgcaaattgg tcgtcattcg
     3121 cgaaacagcc gatacgatta cggatctgat ttcgacgcgc acgagccacc agctggaaat
     3181 ttacatcatc atcctgatcg gcatcgagct ggtgctggct ttgtacgacc gctttctcaa
     3241 gtagcgcgcg cgcccgacaa agcgcggcgg atcatggaat agcgcatcgc gcccgtaatc
     3301 tgcgctgccg ccgcgtacac aaacgccccg gccccgacga gcaccaacag agctatagtc
     3361 tgcacggtcc cgctatggcc cggctcgaaa taaggtgcaa cgacgcggtt cacgtaatag
     3421 agcgccgcac ccatcaacac gctggcaagc aggatgagtg gcgcacgcct gcgcaacgcg
     3481 gcgtcgatct ggaaatgccc gcgccgcatc agggtcgtgg agagcaagat cgcgttcgtc
     3541 cagccggaaa gcgaggtggc tatggcgatg ccgacatggc cgattacgaa gaagagcgcg
     3601 agcgagcctc cgatgtttac ggcgacggag gccatcgcga accacatcgg cgtcttggta
     3661 tcctcgcggg caaaaaagcc gggaaggaac accttcgtca tcacgaaggc aggcagcccg
     3721 cacgcgaaag cagcaagggc ggcggagact tgcaccgtgt cgatcggaga gaacgagccg
     3781 cgctgaaaca acacctgaac gatgggcttc gggattacga tcagcgcgac ggcggcaggc
     3841 agcgtaagca gaagggcaaa ctcaaggctc cggttctggc tcgtcaaggc accggcttcg
     3901 tcctggtccg ccaaatgccg cgacagcgtc ggtaaaagga ctacgcc
//
LOCUS       DATMGX010000244         9020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_400435, whole genome shotgun sequence.
ACCESSION   DATMGX010000244 DATMGX010000000
VERSION     DATMGX010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9020
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400435"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1197
                     /locus_tag="VNR65_03965"
     CDS             274..1197
                     /locus_tag="VNR65_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17866.1"
                     /translation="MGAAPTYARRDALFALVGIAGEVDLDAPDILVEPSPKMDAPVGT
                     SLGTRDRKLPNGTVHKPRQPRPVLTPGASAALRDQLVAEIQDLKHGDDLALWAHRRLP
                     AKNTLRADDARAVEVAYQAVLDAANRDVQAQSVTIPIEPPAPKSHGSENGNPAANGAA
                     AADTEPSTPRTVSPMSKEVRRRNKAHLAFVGAQPCLVCQRSPCDAHHLKFAQPRSLGR
                     KVSDEFTVPLCRDHHRDLHRYGNEIAWWANLQIAPIEVARDLWQTTLLDPDVFDCSQD
                     IAAASRGRNPGGGLGPARNQTGEVRRDDKQE"
     gene            1181..1780
                     /locus_tag="VNR65_03970"
     CDS             1181..1780
                     /locus_tag="VNR65_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17867.1"
                     /translation="MTSKNSHATSASGGSLTAPAELMPFSEPSFLLPGEDLRDYEAIR
                     QMMVDDIQPQTNIEWLWTLDLIELSWEILRYRRLKNRILDTHRVAAIEAILRRLDGEG
                     MPAEAMAMVQMQAKRTAAEWRDDPEAAIDIEARLYRSGFDNIDINAEVFIQAQESFEM
                     FDQLILLAQNRRIGLLREISVRREFLKRARRVIGALDGL"
     gene            complement(1949..2965)
                     /locus_tag="VNR65_03975"
     CDS             complement(1949..2965)
                     /locus_tag="VNR65_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HWJ17868.1"
                     /translation="MSSFSKLLSRSFGWLIAVIWFLFRAVLIAWATLAIYYSNLPFAE
                     VRTGLAGAFALFTIWAFWISRQRRMPVIAMALFLGVVAWWISIPPSHDRNWRPEVAVM
                     PRAVIDSDRVHFTGVRNFDYRTRNDFTPHYEERDVSLSHLTGLDFYVSYFTKGPVGHT
                     FVSFIFDNAPPLSISIETRPEVGEGFAPVASLFKQFELIYVVGEERDLVGVRTNHRRE
                     AVYLYHLNTSAADARRLFLIYLARINELADRPEFYHLLTNSCTINIVRYANASGRAGS
                     FDFRHFLNGLIDAYLYESGRMDTTLPFDELRRRSLINEAAQAADGAPDFSQRIRASLP
                     TIAR"
     gene            complement(3021..3710)
                     /locus_tag="VNR65_03980"
     CDS             complement(3021..3710)
                     /locus_tag="VNR65_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17869.1"
                     /translation="MPLFELPVRLTANVAIESERFTESSDSDVDKIGGSIRLQYVDQK
                     NDQAFSPYIAYAPRFDFIPTFSSSIATRQDVNFGFNKRYNLDGNFQSVPLGGNTSAST
                     VWSFGLTVVAQRRLRDPGPSSSALFIIPSISYVISDQWNASFAIEMIDRRFDSNGVGF
                     FRRDTEVQPIVTLEYVIPSALFGTPETARTFGRPALDFQTSYVRNWSNLPLRDYSQWT
                     ASIVLKTGWRF"
     gene            complement(4197..4547)
                     /locus_tag="VNR65_03985"
     CDS             complement(4197..4547)
                     /locus_tag="VNR65_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HWJ17870.1"
                     /translation="MKEWMIGIGENAVIVINAMGLVVIVIGTIEAFLRSVYAVFIPSG
                     TGEVFRNAYLRYARWLVAGLTFQLAADIIGTSVVPTWVEVGRLGAIAVIRTFLNFFLE
                     RDLTEVEQRPSRDG"
     gene            complement(4744..6237)
                     /locus_tag="VNR65_03990"
     CDS             complement(4744..6237)
                     /locus_tag="VNR65_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWJ17871.1"
                     /translation="MAKTKRPNILILWGDDIGWWNISYNNRGQMGYRTPNIDRVGNEG
                     VAFTDYYGQQSCTAGRAAFITGQNPIRTGLTKVGMPGADIGLKAEDPTIAELLKPHGY
                     RTGQFGKNHFGDKDEFLPTLHGFDEFFGNLYHLNAEEEPEDPDYPKNPEFKKRFGPRG
                     VLHTWADGKGGQKIEDTGPLTKKRMETIDDEVTEHALRFIDDAHKADEPFFVWYNTTA
                     MHFRTHCAEKHKGKSGQGEYNDVMVAHDENIGTMLNKLDELGIAEDTIVMYSTDNGVH
                     YNTWPDAGITPFRSEKNTNWEGGWRVPAFVRWPRKIKAGSVANGIVTHQDWLATFLAA
                     AGEPDIKEKLLKGHTIGTKTYTVCIDGYNMLPYLCGEVEESPRESFFYISDDGEILAI
                     RMQDWKVVLMEQRAKTLQCWFEPFVRLRAPKMFNLRRDPFERADENSNTYWDWVISHA
                     YMVYGMQAIVAGQIENFKKYPPRQKPASFNLDAVLATLEEASGGGNH"
     gene            complement(6301..6744)
                     /locus_tag="VNR65_03995"
     CDS             complement(6301..6744)
                     /locus_tag="VNR65_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HWJ17872.1"
                     /translation="MDNQQLNEESNRFVVRTTAESHFSWIRTRLSVERTIMAYDRTAV
                     ALIGFGFTIVQFFHRMDQIPGTNSARYPDAAWYLGLALIFSGVVSLVVSLWLYFWTLR
                     YLWSGEFAAIAGMTKEGTKSPIIATTFVLIFIGMFAFFAVLLRWI"
     gene            complement(6944..7456)
                     /locus_tag="VNR65_04000"
     CDS             complement(6944..7456)
                     /locus_tag="VNR65_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17873.1"
                     /translation="MAKTETASKNQIILAQLVVALMIAFVALGLAWYGISAGVRERIW
                     QNMLDRPGGPMTFRFILQPIMATIAALFDGVKDARAGRSPYLWTIFFNTEKRGSRLRE
                     GLISTARIILLGLGMDLIYQFIEFKTFHPGEAVITALLLAFVPYLLLRGPCARIARWW
                     RGDTSAIEIR"
     gene            8248..8466
                     /locus_tag="VNR65_04005"
     CDS             8248..8466
                     /locus_tag="VNR65_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17874.1"
                     /translation="MRWFYLTVIVLLAAATFVFAVQNFEIVTVTFLRLSARAPLALLV
                     AIIYLLGMATGGSLLALLRRTIQGASLT"
     gene            8549..>9020
                     /locus_tag="VNR65_04010"
     CDS             8549..>9020
                     /locus_tag="VNR65_04010"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWJ17875.1"
                     /translation="MIDKYTMPEAMGASARRRFHVMAKPSGSACNLDCKYCFYLSKET
                     LPNGPGVGQMDEDTLELFIKQYIEGVTGPEVVFSWQGGEPTLRGLEFFRKVVLLQKKH
                     AKSGQRIENDLQTNGVLLNQEWAEFLKDNHFLVGLSIDGPRELHDRFRVNKGGGP"
BASE COUNT         2037 a         2530 c         2523 g         1930 t
ORIGIN      
        1 cccggaccgc ccggaccggc aggtgctccg gggcccgcag gacccgcggg cgcgacgggt
       61 ccggccggac ccaaggtaga ccccggcgaa gccgctaagg aatagagcag accaaaaacg
      121 cggaggccgc gcccagttgg cggcctcttt gattgcccag cggccgcgcg gcttgaacgc
      181 attcacggcc ccacctcggc gcatgcctcc ggggaatgaa tttcgtcgaa ctggccggtc
      241 tgcccgacca gcgaaaccgc aaaccctcat cgaatgggag cggccccaac ctatgcccgg
      301 cgcgatgcac tgttcgcact ggtcggcatt gccggggaag tcgatctcga cgccccggat
      361 attctggttg agccgtctcc caaaatggat gcgccggtcg gcacaagcct tggtaccagg
      421 gaccgaaaac taccgaacgg aacagtccac aaaccgcggc agccgagacc agtcctgacg
      481 cccggggcat ctgccgcgct gcgcgatcaa ctggttgcag agatccagga cctcaagcac
      541 ggtgacgatc ttgccctatg ggcgcatcgg cggctgccgg cgaagaatac actcagagcg
      601 gacgacgcac gtgccgtaga ggtcgcctat caggctgtac tcgacgcagc caaccgtgat
      661 gtccaagctc aatccgtcac gatccccatt gaaccgccgg caccgaaatc gcatggctcg
      721 gaaaacggaa accctgccgc taatggggcg gccgccgccg atacggagcc gtcgacgccc
      781 cgaacagtct cgcccatgag caaggaggtg cggcgacgga acaaggccca tctcgccttc
      841 gtcggggcgc agccttgcct cgtatgccag cgctcgccct gtgacgctca ccatctgaag
      901 tttgcccaac cacggtcgct cggacgcaag gtcagcgacg aattcacggt gccgttgtgc
      961 cgggatcatc accgagacct gcatcgttac ggcaacgaga tcgcatggtg ggccaatctt
     1021 cagattgcac cgatcgaggt ggctcgggat ctctggcaaa caacgctgct tgatcccgac
     1081 gttttcgatt gctcgcaaga tatcgctgca gcctcgcgag ggaggaatcc gggcggcgga
     1141 ttagggccgg cgagaaatca aacaggcgag gtgcgtcgtg atgacaagca agaatagcca
     1201 tgcaaccagc gcgtctggtg gttctctcac agcgcctgct gaactcatgc ccttctcgga
     1261 gccgtccttc ctgctacccg gcgaggatct ccgcgactat gaggccatcc gccagatgat
     1321 ggtcgacgac attcagccgc aaaccaacat tgagtggctg tggacgctcg atctcatcga
     1381 actctcgtgg gaaatcttgc ggtatcggcg cctgaaaaat aggatcctgg atacccatcg
     1441 cgtagccgcg atcgaagcga ttttgcgacg actggacggt gaggggatgc cggctgaggc
     1501 catggcaatg gtacaaatgc aagcaaagcg gacggccgca gaatggcgcg acgatccaga
     1561 ggccgctatc gacattgaag cgcgtttgta ccgaagcgga ttcgacaaca tcgacatcaa
     1621 tgccgaggtg tttattcagg cgcaagagtc attcgaaatg ttcgatcaac tgatactgtt
     1681 ggcacagaac cgacgtattg ggttgcttcg ggagatcagc gttcgtcgcg agtttcttaa
     1741 gcgcgcccga cgcgttatcg gagcgctgga cggtttgtga tggccccact atgcgtttcg
     1801 ttgcgaccac cacagctgcg ctgaatttcc gccctttgca taacttttga ttccatcgtc
     1861 taaaggaaag tccgagaggc ccgcgcggaa accgcccggg tctaggagca gctagtagct
     1921 cgtcagtctt gttcgccgga cggaagaatc agcgagcgat cgtcggtagg gatgcccgaa
     1981 tacgctgcga gaaatcgggc gcgccatcgg cggcctgtgc ggcctcgttg atcagggagc
     2041 gtcgtcgcag ttcgtcaaat ggcagcgtcg tatccatccg gcccgactcg taaaggtagg
     2101 catctatcag accgttgaga aaatgacgga agtcgaacga accggcccgg cccgaggcgt
     2161 tcgcgtaacg cacgatgttg attgtgcagc tgttggttag caggtgatag aactccggcc
     2221 gatcggcaag ttcgttgatc ctcgctaggt agataaggaa caaccggcgc gcatcggcgg
     2281 ccgaggtatt gagatgatat agatacactg cctcccggcg gtggttcgtg cgcacgccca
     2341 cgaggtctcg ctcttcacct accacgtaga tcaactcaaa ttgcttgaag agagaggcga
     2401 cgggcgcaaa cccttcaccg acctccggcc gtgtctcgat agatatgctc agcggaggcg
     2461 cattgtcgaa gatgaaacta acgaacgtgt gtccgaccgg ccccttggta aagtaggaca
     2521 cgtaaaagtc gaggcctgtt aggtgcgaca acgacacgtc gcgctcttca taatgtggcg
     2581 tgaagtcatt ccgagtgcgg tagtcgaaat tgcgcacacc cgtgaagtga acgcggtcac
     2641 tgtcgatgac agctcgcggc attaccgcga cttccggtcg ccagttacga tcgtgcgagg
     2701 gtggaataga gatccaccac gcgaccacgc cgagaaacag ggccatcgca ataacgggca
     2761 tccgccgttg acgcgaaatc cagaaagccc agatcgtgaa taacgcaaag gcgcccgcaa
     2821 gcccggtacg cacctcggca aaaggaagat tcgaataata gatggcgagg gtggcccacg
     2881 cgatgagcac ggcccggaac agaaaccaga ttactgcgat cagccagccg aaagaccggg
     2941 acaataactt gctaaatgaa ctcaacgcct cctccggctc atctcaactg cgcgacgcaa
     3001 gttgggccaa tatgcattca tcagaaacgc cagccagttt ttagaacgat gctggccgtc
     3061 cattgactat aatccctgag cggcaggttc gaccaatttc ttacatagga tgtctggaag
     3121 tccaatgccg gccgcccgaa agtgcgcgcg gtttcgggag tgccaaacag cgcagaagga
     3181 atcacatatt ccagtgtaac gatgggttgc acctctgtat ctcggcgaaa gaatcctaca
     3241 ccgttagaat caaaccggcg atctatcatt tcgatagcaa aactagcgtt ccattggtcg
     3301 gagataacat aggaaatcga ggggataatg aagagtgcag acgatgaggg gcctgggtct
     3361 cggagccttc gctgggcaac gaccgtcaat ccgaacgacc atacagtcga ggctgaggtg
     3421 ttgcctccca ggggaacgct ttggaagttt ccatcaagat tgtacctttt gttgaaaccg
     3481 aaattgacgt cctggcgtgt cgcgatgcta cttgagaacg tgggaatgaa atcaaatctc
     3541 ggcgcgtagg caatgtaggg cgagaaggcc tgatcgttct tttgatcgac atactgtaac
     3601 cgtatcgagc cgccgatctt gtcgacatcg gagtcgctcg attccgtgaa tctctcgctt
     3661 tcgattgcga cgtttgccgt taaccgcacc ggtaactcga acagcggcac ggcccaagag
     3721 aggtttcccg ccgatccgag ctgcgaggtg ggcgttccgc cagctcttat ctcttccgca
     3781 tttgaattat agccgaacgg gaccaagaga ttgaatctta gctgcgacga tggcgcctgc
     3841 tgctccaagc cgggcgcagt cgcgatcaaa ttcgtctgtg gtgctgacgt cggttggccc
     3901 ggtgttgcaa ggattgcgtc aatgcgtgca tcctgcgcgt cgctggtggc tgattgcgct
     3961 gctcgcgttg acggcagact caggattgag aggatcgcgg tcgctgcgaa agcggacccc
     4021 ctggtcgaaa gccgtcgttt tcgccagacg cgaggtctct gatcacgttt cctggcgccg
     4081 ccagatcgaa ccatttcatg ggccgtcatg ctaaatacca ggccaggacg ccgcataact
     4141 agacgcgtcg cttctatcta tggggctgca gtgagagtct tgaagcggct tgacgatcac
     4201 ccatcgcgac tcggccgctg ctccacttca gtcagatcac gctcaaggaa gaaattgagg
     4261 aacgttcgga tcactgcgat ggcgccaagg cgtccgactt caacccaagt tggcacgacc
     4321 gaagttccga tgatgtccgc agccagctgg aaagtgagcc cggcaaccag ccagcgcgca
     4381 tagcgcaaat aagcatttcg aaacacctcg ccggtgccgg aagggatgaa aacagcgtaa
     4441 acgcttcgga ggaatgcctc gatggtcccg atgacgatca ccaccaaccc catcgcgttg
     4501 atgacaatga cggcgttttc gccgatgcca atcatccatt ccttcatcag atccctcccg
     4561 gacgacagcg attacggcag tttccggcat ctgcgacgca ttatgtctca tagggtcccc
     4621 gaagcgtgcg gcgcttcgga cgttttttgc gattcttgga atttgatcca tgcacggtca
     4681 gccgtgcatg gatcgatctc ggtcttgagt aagaacgacg gtcgatcggc gaccagcgaa
     4741 cggttaatgg ttaccgccgc cgcttgcctc ctccagcgtt gccaacacgg cgtccaggtt
     4801 gaacgaggca ggcttttgac gaggtggata ttttttgaag ttctcgattt gcccagccac
     4861 aatggcctgc attccataaa ccatataggc gtgagagatc acccagtccc agtaggtatt
     4921 cgaattctcg tcggcccgtt cgaacgggtc ccgtcgcaag ttgaacatct tgggagctct
     4981 taagcgtacg aatggttcga accaacactg cagcgtcttc gcccgctgct ccatcagcac
     5041 tactttccag tcctgcattc gaatggcaag aatttctccg tcatcactga tgtagaagaa
     5101 tgactccctg ggactttcct ccacttcacc gcacagatac ggcaacatat tgtagccgtc
     5161 gatacagaca gtgtaggtct ttgtgccgat cgtgtggccc ttgagaagct tctccttaat
     5221 gtctggttca ccggcagccg ctaagaatgt cgccagccaa tcctggtggg ttacgatgcc
     5281 gttcgccacc gagcccgcct tgatcttgcg cggccaccgt acgaaggcag gcacgcgcca
     5341 accgccctcc cagttggtgt ttttctcaga gcggaacggc gtgataccgg catccggcca
     5401 agtattgtag tggacgccgt tatccgtcga gtacatcacg atcgtgtctt cggcgatgcc
     5461 aagctcatca agcttgttca gcatcgtgcc gatattctcg tcgtgggcga ccatcacgtc
     5521 attgtattcc ccttgaccac tcttgccctt atgcttctcg gcgcagtgag tacggaaatg
     5581 catggctgtg gtattatacc agacgaagaa cggctcatcc gccttatggg catcatcaat
     5641 aaagcgcagc gcgtgttctg tgacttcgtc gtcaatggtt tccatccgct tttttgtcag
     5701 cggtcctgtg tcctcgattt tttgcccacc cttgccgtca gcccaggtat gaagaacgcc
     5761 tctgggccca aaccgcttct tgaactcggg gttcttagga taatccggat cttcgggttc
     5821 ttcttcggca ttaagatggt aaaggttacc gaaaaattcg tcgaagccgt gcaacgtcgg
     5881 tagaaattcg tccttgtcgc caaagtggtt cttgccaaat tgaccggtcc tgtagccgtg
     5941 cggctttaac aattcggcga tggttgggtc ttccgccttg agtccgatgt cagctccggg
     6001 catccccacc ttggtcaacc ccgtgcgaat cgggttctga ccagtaatga aggctgcgcg
     6061 gcctgcggta cagctctgct ggccgtagta gtccgtgaag gccacgcctt cgttgcccac
     6121 gcggtcaatg ttgggcgtgc ggtagcccat ctggccgcga ttgttgtagc tgatgttcca
     6181 ccagccgata tcgtcgcccc agaggataag gatgttgggt cttttcgttt tagccatttg
     6241 actctcccgt gttgaactct cgcgtttcgt ttaaattagt atctttgcgg cggagcggcg
     6301 ctaaatccag cgcagtagca cggcaaaaaa tgcgaacatg ccaataaaga tcagaacgaa
     6361 cgttgttgcg ataatcggcg acttcgttcc ctcctttgtc atgcctgcga ttgccgcgaa
     6421 ttccccgctc cacaggtaac gaagcgtcca aaaatatagc cacaacgaca ccacaagcga
     6481 cacaacgccg gaaaagatca gcgcaagccc gaggtaccac gcggcgtccg gatagcgggc
     6541 ggaattagtc cccggaatct gatccatgcg gtggaagaac tggacgatcg taaagccgaa
     6601 cccgatcaac gcgaccgccg tgcgatcgta cgccataatc gtgcgttcga cggagagccg
     6661 cgtccggatc caggaaaaat gactttccgc tgtcgtgcga acgacaaacc gattggactc
     6721 ttcgtttaac tgctggttat ccatcgcttt tacatccgca attctgttgc tcgcgctccg
     6781 aacaggaagt catcgaccgg cggacaggat agaattcgat cggtagccca ctctcgaaca
     6841 aggatcgtcc gtttgacgca caccgttcat gttgaagcgg ccctcaagat agagaattca
     6901 tgataacgat tacttcgggt aagtcacctg catcctcgcg ccctcatcga atttcgattg
     6961 ctgacgtatc accacgccac cagcgcgcga tgcgcgcgca cggtccacgc agcaacaggt
     7021 aggggacaaa ggcgagcaag agggcggtga tgaccgcctc accgggatga aaagtcttaa
     7081 actcgatgaa ctgatagatc aggtccatgc cgagacctaa aagaatgata cgagcggtgg
     7141 agatcaatcc ttcgcggagg cgactgccgc gcttttctgt gttgaaaaat atcgtccaaa
     7201 gatagggaga gcgcccagct cgagcgtcct ttactccgtc aaacagcgct gcaatcgtcg
     7261 ccataatggg ctggagaata aaacggaagg tcattggccc gcctggacga tcaagcatgt
     7321 tttgccagat gcgctcgcga accccggctg aaatgccata ccacgccaag ccaagagcga
     7381 cgaacgcgat catcagggcg acgaccaact gagccaatat aatttgattt tttgatgccg
     7441 tttcagtctt cgccaaggct cccacctacc gcttgatggt atattataag accaaatgac
     7501 atcaaatcaa ttgagatgga tcaacgtcct cccgctgcac caaggttgtc aaggaccggg
     7561 cttaacgcta tgcaacgtcg gcagaaagag accacatttc tgctcatatc agcgccaact
     7621 ttgcatcggg ggcaggactt cgacgcaggt cggcgcgata gaaatgctaa acaaaaatgt
     7681 gtccggaggt tggcaccatc gagccgttca tcgtcaatag tgcagctacg gctgaaactt
     7741 gcggtagcat ttatgctcta accacgctga tcgaacagcg ctcggcccca gcttccattc
     7801 gctgcttgac ttacgtcaat aggctggaag agcaggtgtg tagaggatta tttaaaagct
     7861 ctgaaatgtc tgttggtcag tttgaggagg aagcgatttg aaataccaaa actgcggaag
     7921 gcggaagtta gctgtctcgg ctggtttagt gatgcttgga tctttgttgg cgatctcaac
     7981 ctctttcgct caatcgccgg aaaattgccg ggcctatgct gaggattact cttatcgcta
     8041 ttcggcgccg tgggcgtcgc tgaatttcgg tatcgggggg cggcgcgccg gcgttagtgc
     8101 acttgccgcc aacagggcgc acggcataga aaagtctaag ctctttgaca acgcctacgc
     8161 acgctgcatg cgtggtcgat ggccgtgaga gtccggctgt tctcaaccac gcgctaggtt
     8221 tctccctaag agataggacc tacgaccatg cgatggtttt acctaaccgt gattgtgctg
     8281 cttgctgctg ccacttttgt ttttgcggtc caaaacttcg agattgtcac cgtcacattt
     8341 ctcagactca gcgctcgcgc accgctagcg ctgctggttg ctataattta tctcctcggc
     8401 atggcgacgg gcgggagcct gctggcgctg ttacgtcgca caattcaagg agcaagtctc
     8461 acttaggttc gcctttggaa ggaccgatag ctcttgctgt cggcaatacg gcgagccagc
     8521 cataccagga ccctcggagc cggaatttat gattgacaaa tacaccatgc ccgaagcgat
     8581 gggcgcctcc gcccggcgac gatttcacgt catggcaaag ccgagcggat ccgcctgtaa
     8641 tctggattgc aagtactgtt tttatctcag caaggagacg ttgccgaacg ggcctggcgt
     8701 cggccagatg gacgaagaca cccttgagct ctttatcaag caatatatcg aaggcgttac
     8761 gggtccagag gttgtctttt cctggcaagg aggcgagccg acgctccggg gtcttgagtt
     8821 ttttcgtaag gtagttttac tgcagaaaaa gcatgcaaag tccggccaac gcattgaaaa
     8881 cgatctgcag acaaatggcg ttctcctgaa ccaagagtgg gcggagtttc tcaaagacaa
     8941 tcactttctt gttggtttga gtatcgacgg tccacgcgag ctgcatgatc gatttcgtgt
     9001 caataaaggc ggcgggccga
//
LOCUS       DATMGX010000245         2579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_255863, whole genome shotgun sequence.
ACCESSION   DATMGX010000245 DATMGX010000000
VERSION     DATMGX010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255863"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VNR65_04015"
     CDS             <1..834
                     /locus_tag="VNR65_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWJ17876.1"
                     /translation="LRGKVVGGNTAIYSPSGGSVGIAFAIPAATARDVVQKLRDQGKV
                     SSGWLGVQIQNVDENIAESQGLKEAKGALVAKILPEGPAAKSELKSRDIIQQVNGKAV
                     TNSRELARTIAALPPSTDARLHIFRNGEERDVTVKLGEFPANDKVAALGGPGGNEENE
                     GGKELEDLGLTLVPAPEFPGSKSKEGVAVSGVDPNSKAADQGLKRGDVIIEVNGKPVS
                     TPDDVSDTIRDAREKGRKNVLLQIRSRDQQRFLALPIDGKPEINKDEETSKEPQKRGK
                     R"
     gene            1011..1691
                     /locus_tag="VNR65_04020"
     CDS             1011..1691
                     /locus_tag="VNR65_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ17877.1"
                     /translation="MRVLIVEDDRTAADFLAKALAEAGYQPEIAGDGETGLELAMSST
                     FGVLIVDRMLPKLDGLSLVERLRSRGDRTPVLILSALGAVDDRVKGLRAGGDDYLPKP
                     YSLVELVARVEALARRGPSEGAVTTYAVGDLVLDRLSHKVTRAGEAILLQPREYRLLE
                     YLMRHAGQVVTRTMLLENVWDYHFDPQTNVIDVHISRLRSKIDKGQSKPLLETVRGAG
                     YMIRDDRS"
     gene            1678..>2579
                     /locus_tag="VNR65_04025"
     CDS             1678..>2579
                     /locus_tag="VNR65_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HWJ17878.1"
                     /translation="MTAADVVRLVPRLILATPFRLFAVYVSLFCAAVAAVFIYVNIAM
                     LGFLSQEAETSVRGDYDFLSARYRDNGLPALVNAISEHAASSNNALYLLTDGKGRWLA
                     GNLDTVAANLWETQGEAQFSFRNRGNEQSERPAFGIVARLPGDLHLLVARDIESQEAL
                     KRLLRRAFILGYALIFIIGTAGAVIVSRRMLRRVDIASNAARSIMQGNLAQRIPVTGR
                     SDEFDRLSQNLNAMLDRIQDLMIGLKNVSDNIAHDLKTPLHRLRTRAERAMQSSRSPE
                     KLAEALGSVIEEADTLIQTFDALL"
BASE COUNT          552 a          779 c          746 g          502 t
ORIGIN      
        1 cttcgcggca aggtggtcgg cgggaacacc gccatctata gcccgagcgg cggcagcgtc
       61 ggcattgcgt tcgcaattcc tgcagcgacc gcccgcgacg tcgttcaaaa gctcagggac
      121 cagggcaagg tcagcagcgg atggcttggc gttcagattc agaacgtgga tgagaatatc
      181 gccgaaagcc aggggctgaa ggaagccaaa ggcgcgctcg ttgcgaagat tctgccggag
      241 ggccctgccg cgaaatccga actgaagtcg cgcgacatca tccagcaggt caacggaaag
      301 gccgtcacga actctcgtga actcgcaagg acgatcgccg ccctgcctcc cagcacggat
      361 gcccggctgc atatcttccg caatggcgaa gagcgcgacg tcaccgtcaa gctcggcgaa
      421 ttcccggcca acgataaggt cgcagccctc ggcggccccg gcggtaacga agagaacgaa
      481 ggcggaaaag agcttgaaga tttgggcctg acgctggttc ctgcccctga gtttcccgga
      541 agcaaatcga aggaaggcgt cgcggtttcc ggcgtcgatc cgaattcgaa agctgccgat
      601 cagggtttga agcgcggcga cgtcatcatc gaggtcaacg gcaagcccgt atcaactccc
      661 gacgatgtgt cggacaccat tcgggatgcg cgtgagaaag gccggaagaa cgtactcctt
      721 caaattcgca gccgcgacca gcagcgcttc ctcgcccttc ccattgacgg caaacccgaa
      781 ataaacaagg acgaagagac cagcaaagag ccgcagaagc gcggcaagcg ctaacaagtc
      841 agggcgaggg cttttgccct cgcccttcta gagcagtccg cgtccatttt ggacatgtat
      901 aagctgctcc agcactttag aaacgatcaa cgtgtccgcc tttaatcgat tccgctttaa
      961 ggcgggttga tctagcagct gggttgtttt catctaggaa tggtgctcgc atgcgagttc
     1021 tgatcgttga ggacgaccgc acggcggccg acttcctcgc aaaagcgctg gctgaggccg
     1081 gctatcaacc cgaaattgca ggcgatggcg agaccggcct tgagcttgcg atgtcctcca
     1141 cattcggcgt tcttatcgtg gacaggatgc ttccgaagct cgacggcttg tcgctggtgg
     1201 agcgtctgcg ctcccgaggt gatcgcactc cggtgctgat cttgagtgcg ctcggcgctg
     1261 tcgacgaccg cgtcaaaggc ctgcgcgccg gcggcgacga ctatctgccg aaaccgtatt
     1321 ccctggtgga acttgttgcg cgcgttgaag cacttgcgcg gagaggcccc tcagagggag
     1381 ccgtgacaac ctacgccgtg ggcgaccttg tgctcgaccg cctttcgcat aaggtcacgc
     1441 gcgcgggcga ggccatcttg ctgcaacctc gggaatatcg ccttctcgaa tatctcatgc
     1501 ggcatgccgg ccaagtcgtg actcgaacca tgctcctgga aaatgtgtgg gattatcatt
     1561 tcgacccaca gaccaatgta atcgacgttc atatttccag acttcgcagt aaaatcgaca
     1621 aaggacaaag caaaccgctt ctggaaacgg tgcggggcgc gggttacatg atccgcgatg
     1681 accgcagctg atgttgtaag acttgtcccc cgcctaatac tcgcaacgcc tttccggctt
     1741 ttcgcggtgt atgtctcgtt gttttgcgct gccgttgctg cggtgtttat ttacgtcaac
     1801 atcgccatgc tcggatttct cagccaggag gcggagacct ctgtgcgggg cgattacgat
     1861 tttctttcag cgcgttaccg cgacaacggg ctgcccgcgc tcgtcaacgc gatctctgag
     1921 catgcggcct cctcgaacaa cgctctttat cttctcaccg acggcaaggg ccgatggctt
     1981 gccggcaacc tcgacaccgt cgccgccaat ctgtgggaaa cgcaaggcga ggctcaattc
     2041 tccttccgca atcgtgggaa cgagcaaagc gagcgcccgg cattcggaat cgtagcccgg
     2101 cttccaggcg atcttcatct cctcgtcgcc cgcgacatcg aaagccagga ggccctcaag
     2161 cggctgttga gaagagcctt catccttgga tacgccctca tcttcatcat cggaacggca
     2221 ggcgctgtca tcgtgagccg ccgcatgctg cgcagggttg acatcgcatc aaacgcagcg
     2281 cgctcgatca tgcaaggcaa tctcgctcag cgtattccag ttacgggacg cagcgacgag
     2341 ttcgaccgct tgagccaaaa tctgaacgcc atgctggacc gcattcagga cctcatgatc
     2401 gggctcaaga acgtgtcgga taacatcgcc cacgatctga agacgccgct tcaccgcctg
     2461 cgcacaaggg ccgagcgggc aatgcaatca agccgctctc cggaaaagct tgcggaagcc
     2521 ctaggctccg tcatcgaaga agcggacacg cttatccaaa ccttcgacgc gctcctgag
//
LOCUS       DATMGX010000246         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_350137, whole genome shotgun sequence.
ACCESSION   DATMGX010000246 DATMGX010000000
VERSION     DATMGX010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350137"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VNR65_04030"
     CDS             complement(<1..1501)
                     /locus_tag="VNR65_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWJ17879.1"
                     /translation="MLRNDHALFRSVFGHARVGMVAADPAQRLVHCNRAFCAMLGYGH
                     EEIIGLRLADIVAPEDIARIEPELVHSSGEQASHSKWRFQRKDGSSFPGAILSDRSQD
                     GWLQAIIVDSTESNRPEATCFAQESKDRYFSKLEKQLREAGTARETVSAACEAIGREF
                     GASFAAIGEVHRDGEHAIVDNAWSAIGDVTPLLGWHGRSRAERIAAYMTAGAVAVEDV
                     LTDPRLSGNAEVQATCKAAGVRSSIAIALPREGRPWAILFVADGIRRAWTEAEIALAQ
                     ETLERVWHDVERARTEEELRLTTERFQLALKGSPVSLFCQDLDLRYTWISNPQLASPP
                     SVGKLPGEVFERAEDAAASEAIKREVIRSGRSRRQEFAVRSGGVERIYDLLVDPLRDA
                     DGNMTGVKCAAIDITALKQAEAALRESKERQAFLLALNDALRRIFDPLEIMATASRLL
                     GSKLAAGQVAYADIDEAGEHAVISREWNDGTIPIKAAVYGIENFGANLKR"
     gene            complement(1749..>2725)
                     /locus_tag="VNR65_04035"
     CDS             complement(1749..>2725)
                     /locus_tag="VNR65_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWJ17880.1"
                     /translation="QRMSVKLRTIVGDALAAATHVSTGSGELSAASHEVSAGANEQAA
                     SAEEVSSSMEEMSANIKQNADNAAHTEEIARRSWTDAQESGDAVNRAVVAMQTIAEKI
                     TFVQEIARQTDLLALNAAIEAARAGEHGKGFAVVASEVRKLAERSQTAAAEIGMLSGQ
                     TVAAASEAGAMLAKLVPDIKKTAELVEEISAACREQDVGANQVNQAIQRLDKVIQQNA
                     GAAEEMSATSEALSGQAEHLLASIAFFRIDGKAGAASKNEKPLVPAAALPQTKTPAPA
                     QPRAAESANSARMRLKSRSDYKGRHGNGFALNLSNGRADDFDADFESF"
BASE COUNT          509 a          871 c          784 g          561 t
ORIGIN      
        1 ctcgtttcaa gttggcaccg aaattctcga tcccatagac agcggctttg atcgggatcg
       61 tgccatcgtt ccattcgcgc gaaatgacgg cgtgctcgcc tgcctcgtca atatccgcgt
      121 aagcgacttg cccagcggca agcttgctcc ccagcaacct gctcgccgtc gccatgatct
      181 ccaacggatc gaaaatccgg cgaagtgcat cgttgagggc gagcaggaac gcttggcgct
      241 ccttgctctc tcgtagcgcg gcctccgcct gcttaagggc ggtaatatcg attgccgcgc
      301 acttgacgcc tgtcatattg ccatcggcgt cccgcagagg atccaccaaa agatcgtaaa
      361 tccgttctac gccgccagac cgaacggcga actcctgcct tcggcttcgg cccgagcgga
      421 taacctcgcg cttgatagcc tcgcttgccg ctgcatcctc ggccctctca aagacttcgc
      481 ccggcagctt tccgacggat ggtgggctgg caagttgagg attggaaatc cacgtatagc
      541 gcagatcgag atcctggcag aaaagcgaga ccggcgagcc tttcagagcc aattgaaagc
      601 gctccgtcgt cagccgcagc tcttcctcgg tacgcgcccg ctccacgtcg tgccagacgc
      661 gctcgagcgt ctcttgcgca agggcgattt cggcctcagt ccaggcgcgc cgtatgccgt
      721 ccgcaacgaa taagatagcc cagggacggc cttcacgcgg cagcgcaata gctatcgaag
      781 accttactcc agccgccttg caggttgcct gcacttccgc gttccctgag aggcgagggt
      841 ccgtcaagac gtcttcgact gcgacggctc cggcagtcat ataagctgct atgcgttcag
      901 ctctcgaacg cccatgccac ccaagaagcg gtgtgacatc tccaattgcg ctccaggcgt
      961 tatcgacaat ggcgtgctcg ccatcgcggt ggacctcgcc tatcgcagcg aaggaagcgc
     1021 caaactcccg gccgattgcc tcgcatgcgg cgctgaccgt ctcgcgcgcc gttccggcct
     1081 cccgaagctg cttctccagc ttggaaaagt aacgatcttt gctttcttga gcaaagcagg
     1141 tggcctcggg ccggttggat tctgtgctgt caacgatgat cgcttgcaac cagccgtcct
     1201 gcgatctgtc gcttagaatt gcgccaggga aggacgagcc gtccttgcgc tgaaaccgcc
     1261 atttgctatg agaagcttgt tcgccggacg aatggacaag ctcgggctcg atacgtgcaa
     1321 tgtcctcagg cgcaacgata tcggcaaggc gcaggccaat gatctcctcg tgcccatagc
     1381 cgagcatggc gcaaaaggcc cgattacaat gcacgagacg ttgtgcgggg tctgcggcga
     1441 ccatgcccac cctggcatgt ccgaaaacgg aacgaaacaa agcatggtca tttcgaagca
     1501 tgcggtcctc caccctcgcc tacgatcaac cctgggttgc cagcagtaat cccgctggcc
     1561 cccctacggc aaggccaaaa gacgcagaca cataagctcg gaaatcctct tccgtctttg
     1621 ctgtcgcccg gaggatatcg caatattgtt tggctatcgc taacgaagac gaaagggtgt
     1681 gggcgaacct acgaagaata aagtcgcaag ccagaatcta cagagtcgga gttcggctcc
     1741 cctgacggtc aaaagctttc gaagtcggcg tcgaagtcgt cggcgcggcc attgctgaga
     1801 ttgagagcga agccattgcc atggcggccc ttatagtccg atcgactttt caggcgcatc
     1861 ctcgcactgt tggcgctttc tgcggctctt ggctgcgcgg gggccggcgt cttcgtttgc
     1921 ggaagtgcgg ccgcggggac aaggggcttc tcgttcttgc tcgcggcacc agcctttccg
     1981 tcgatgcgga agaatgcgat actggccagc aaatgctcgg cctgaccaga cagcgcctcc
     2041 gaggttgccg acatctcctc ggccgcgcct gcattctgct ggatcacctt gtcgagtcgc
     2101 tggatggcct ggttcacctg attggcgcca acatcctgct ctcggcaggc tgcgctgatc
     2161 tcttccacaa gctcggccgt cttcttgatg tcgggcacga gcttcgccag catggcgccg
     2221 gcttcgctag cggccgcaac ggtctggccc gagagcatgc cgatctcggc agccgccgtc
     2281 tggcttcgct cggcgagctt gcgcacctcc gaggcgacaa ccgcaaagcc cttgccatgc
     2341 tcgcctgcgc gagcggcctc aatggctgcg ttcagcgcaa gaagatcggt ctggcgggca
     2401 atttcctgca cgaatgtgat cttctcggcg atggtctgca tcgccactac tgcacgattg
     2461 acggcgtccc cactctcctg cgcgtcagtc caggatcgcc gcgcgatttc ttccgtatga
     2521 gccgcgttgt cggcattctg cttgatgttg gcagacatct cttccatgga cgaggagact
     2581 tcctcggccg aggccgcctg ctcgttggcg ccagccgaga cttcgtggct tgcggcagaa
     2641 agctccccgc tcccggtcga gacatgagtg gccgcggcca gagcatcgcc gacgatggtg
     2701 cggagcttca cgctcatgcg ctgta
//
LOCUS       DATMGX010000247         2584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_135767, whole genome shotgun sequence.
ACCESSION   DATMGX010000247 DATMGX010000000
VERSION     DATMGX010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135767"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..655
                     /locus_tag="VNR65_04040"
     CDS             329..655
                     /locus_tag="VNR65_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17881.1"
                     /translation="MAATLTAPAVETYDTAFPAEWSYYEYNRGVNAVVTMTEAAPGHK
                     LLWGTESTVPEIFTGSAITSRRMLADALQQRVDTGSPPGKLTTPLAERILYKNAEKLS
                     GMNIDA"
     gene            723..2450
                     /locus_tag="VNR65_04045"
     CDS             723..2450
                     /locus_tag="VNR65_04045"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWJ17882.1"
                     /translation="MPAAPVVIYTAKKIVTMERGNPEATAVVVSGKQIVLAGSLEEIK
                     QFLGDKPYLVDDTFASKVIMPGFIDQHLHPLLGALTLAVEVIAPEDWIVPSRIWKKAS
                     NQQQYLSRLKDANAGLVDPNEWLFTWGYHQYFHGPLERKTLDSISATRPIAVWHRSCH
                     EFYLNTFALNSLGVSEDSIKGKGKWSEQTDWAKGHFYEGGLNLIMAQLLPKLATPERF
                     SFGLKQMAKMLHHNGVTAFNEPGALVTPQIFKLYQEILGAEDTPFYSFFIADGRGIVD
                     KYGLEGALAETEKTIALAPPGSGKLQFFEKQIKLFADGAIVSQLMQMKDGYTDGHHGE
                     WMIPPEELARRATFYWNAGYQIHTHVNGDEGLEVVLNILEKLMRDSPRADSRSVIVHF
                     ANSTEEQIARIARLGAIVSANPYYPVGFADKYSEVGLGPERANAMVRSASVVNRGIPY
                     SFHSDLPMGPAAPLYLAWCGINRITNEGHIAGPEQRISVDAGLRAITIEAAYSWRKEH
                     LLGSIAPGKIANFTVLEQDPYAVAPEDLKDIPIWGTVFEGRLFPIHVGASPTASSDEV
                     EQLASCAAQ"
BASE COUNT          589 a          743 c          722 g          530 t
ORIGIN      
        1 ctcatggagc ctccaagaat tggtcgcgga acgagtgatc ggcaagcgga gagggacgtc
       61 ggatgcgcgg ccaggtgatc cgagtcgtac cggattgtcg cctgacgcga ctcaaatcag
      121 gaaggtcgcg tgtcacctag atgtgtgact atcttgcact ttagatcaca agagtcgcgt
      181 ctctgacagt catgtaagtg accaccatga ctggaactta tgtgacattg gtcactgaac
      241 aaattgacgc agcagagtaa cctccagcta atttgttcgc agataaactg cgacgcatgc
      301 gccactaaac gctgttctgg agagtctctt ggccgccact ctgactgcac ctgccgttga
      361 gacctatgac actgcgttcc ctgcggaatg gtcgtactac gaatacaacc gcggcgtgaa
      421 cgcggtggtt acgatgaccg aagccgcgcc tggacacaag ttgctctggg ggaccgagag
      481 caccgtgccg gagatcttca ccggatcggc aatcacctcg cgccggatgc tggccgatgc
      541 gctgcagcag cgcgtcgaca ccggttcgcc gcccgggaag ctaacaaccc cgctggctga
      601 acgcatcctc tacaagaacg ccgaaaagct atccggcatg aacattgatg cctagtccat
      661 ttttctcgag gagcctgttg actatgtgcg caaatcccgt gatggaacac ttcgaatcga
      721 caatgccagc cgccccagtc gtcatctaca cggcgaagaa aatcgtcacc atggagcgag
      781 gcaatccgga agcgactgcc gtcgtggtct ctggcaagca gatcgtgctc gccggatcgc
      841 tggaagaaat caagcagttt ctcggcgaca aaccctacct ggtggatgac actttcgcct
      901 ccaaggtcat catgccggga ttcatcgatc agcatctgca ccctttgctc ggcgccttga
      961 cgctcgcggt cgaggtgatt gcgccggaag actggatcgt ccccagcagg atatggaaaa
     1021 aagcctcaaa ccagcagcaa tacctgagtc ggctcaagga tgcaaacgcc ggcttagtcg
     1081 atccgaacga atggctgttc acctggggtt atcaccagta ttttcacggg ccgcttgagc
     1141 ggaagacgct cgactcgatt agtgcgacgc gacccatagc ggtgtggcac cgttcctgtc
     1201 acgagttcta cctgaacacc tttgccctga actctctcgg tgtcagcgaa gactcgatca
     1261 agggcaaagg gaagtggagc gagcaaaccg attgggccaa gggccatttc tacgagggcg
     1321 gcctgaacct gattatggca cagcttctgc cgaagctggc cacgccggag cgctttagct
     1381 tcggcctgaa gcagatggcg aagatgctgc accacaacgg cgtcaccgcc ttcaatgaac
     1441 cgggcgctct ggtcacgccg cagatcttca agctctatca ggaaattctg ggagccgagg
     1501 acacgccgtt ttacagcttc ttcattgcgg atggacgcgg cattgtcgac aagtacggtt
     1561 tggaaggcgc cctggcggaa accgagaaga cgattgcgct agcgccgccc gggagcggca
     1621 agctccagtt ctttgaaaaa caaatcaagc tctttgccga cggcgccatc gtctcgcagc
     1681 ttatgcagat gaaggatggc tacacggacg ggcaccacgg cgaatggatg atcccgccgg
     1741 aagaacttgc tcggcgagcg accttctact ggaacgccgg ctaccagata cacactcacg
     1801 tcaatggcga cgagggactc gaagttgtct tgaatattct ggaaaagctc atgcgcgatt
     1861 ctccccgcgc tgacagccgt agcgtcattg tgcactttgc aaactcgacg gaagaacaaa
     1921 tcgcgcgcat cgcgcggctt ggcgcgatcg tcagtgcaaa cccctattac cctgtcggtt
     1981 ttgccgacaa gtacagcgaa gtcggcctag gacctgagcg tgccaatgcg atggtgcgta
     2041 gcgcctcagt cgtcaatcgc ggtatcccat actcattcca ctccgacctg ccgatgggtc
     2101 cagccgctcc gctttacctg gcgtggtgcg ggataaaccg gatcacgaac gagggtcata
     2161 ttgctggacc tgagcagcgt atctcggtcg acgctggcct gcgcgccatc accatcgagg
     2221 ccgcttactc ttggcggaag gaacacctgc taggcagcat tgctcccggc aagatcgcca
     2281 acttcacagt ccttgagcag gatccctacg ccgttgcacc ggaagatctg aaagacattc
     2341 caatttgggg caccgtcttc gaagggcgac ttttccctat tcatgtcggc gcatctccca
     2401 ctgcaagctc cgacgaggtc gagcaactcg catcatgcgc tgctcaatag gaagcggctc
     2461 agggctggaa cagaatcacg atgcgcaaca aaatgggttt gatctttctg tcgaatttct
     2521 tttcgggggc ctggttaccg ggctacatat tttgaaaagg aatctgccgt gaaacgaatt
     2581 ttgc
//
LOCUS       DATMGX010000248         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_44812, whole genome shotgun sequence.
ACCESSION   DATMGX010000248 DATMGX010000000
VERSION     DATMGX010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44812"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VNR65_04050"
     CDS             <1..316
                     /locus_tag="VNR65_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17883.1"
                     /translation="SSPSLSDLAASRRLSAPRAFSKRMRLWRIAEESVMGKNLSEENE
                     TAKRDSEGWSRFERAVDAAVKSGLSTTETKCAKRRRESVPARLTEKGFEAPFYNGAFV
                     SI"
     gene            complement(313..1161)
                     /gene="istB"
                     /locus_tag="VNR65_04055"
     CDS             complement(313..1161)
                     /gene="istB"
                     /locus_tag="VNR65_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HWJ17884.1"
                     /translation="MTMLHETPTLTAVAPQMLLGNHLKALKLPTFAREYEKVAMESAQ
                     DRADYPRYLLRLCELERIDRERRNVERRIRLARFPQTKSLDTFDFTAQPSLNKALVLE
                     LARCEWIEKRQNCIALGPSGTGKTHAALALGLAACQRGHSVAFTTAAALVHELMEARD
                     ERRLRALQKHLNTVKLLIVDELGYVPFTAVGSELLFEVFSQRYERGATLVTSNLPFDE
                     WTSVFGSERLTGALLDRLTHHVHILEMNGESFRLATSKKAQRRPGHGQNAGPKTLNEG
                     DPITEN"
     gene            complement(1158..2684)
                     /gene="istA"
                     /locus_tag="VNR65_04060"
     CDS             complement(1158..2684)
                     /gene="istA"
                     /locus_tag="VNR65_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HWJ17885.1"
                     /translation="MFAVELYAKIRRSVMVDGMSRREAAKQFGVHRNTITKMLQFSIP
                     PGYQRRERPASKKLGPDMAWIDAILEGDRSVHKKQRHTAHRIFERLRDERGFSGGYTI
                     VREYVAQARLRAREMFVPLSHRPGHAQADFGEADGYIAGKKVRFHYLCVDLPHSDGCF
                     VKAYPAETAEAFCDGHVAAFDVFGGVPQSILYDNTRLAVAKIVKGGQRLRSQMFAELQ
                     SHYLFNDRFGRPGKGNDKGKVEGLVGYVRRNFMTPLPVAESFEALNARFLDACTKRRQ
                     AILRGHTTTIAERMQADVAAFMPLPPAPYDACHKVATRVSSLSLVRYKNNDYSVPTRY
                     GHQEVLAKGCVDRVEIACRGETIAMHSRSYDTADFVFNPLHYLALLEHKSKALDQAAP
                     LDNWQLADCIHRLRRLMEARMGNAGRREFIQVLRLMEDFHQHQVEHAVAQALRLGAIS
                     FDAVKMLLLARLENRPARLDLTFYPYLPAATVGTTDPRAYLGLIASSGSIPVTAGVPA
                     "
BASE COUNT          598 a          876 c          864 g          594 t
ORIGIN      
        1 gtcctctcct tcattgagcg acttggcggc ttctaggcgc ttaagcgcgc cgcgggcctt
       61 ttctaaacga atgagacttt ggcggattgc ggaagagtcg gtcatgggta aaaacttgtc
      121 tgaagagaac gagaccgcca agcgcgacag cgagggttgg tccaggttcg agcgcgccgt
      181 agacgctgcg gtcaagagcg ggctgtcaac gacggagaca aaatgtgcca aacggcggcg
      241 tgaaagtgta ccagccaggt taacagaaaa gggctttgaa gccccgtttt ataatggtgc
      301 ttttgtgtcg atttaatttt cggtgatggg gtctccttcg tttagtgtct ttgggccggc
      361 gttttgccca tgacctggcc gccgctgcgc ctttttgctg gtggcgagcc taaagctctc
      421 gccgttcatc tcaagaatgt ggacatgatg cgtgaggcgg tcgaggagcg cgccggtgag
      481 ccgttccgag ccgaaaaccg aagtccattc atcgaatggc aggttgctgg tgacgagcgt
      541 tgcgccgcgc tcatagcgct ggctgaacac ctcgaagagc agttctgaac cgacagccgt
      601 gaatggcacg tagcccagtt cgtcgacaat cagcagtttg actgtgttga gatgcttttg
      661 cagcgcccgc agacgacgtt cgtcacgcgc ttccatgagt tcatgcacga gagcggcggc
      721 cgtcgtgaag gcgacgctgt ggcccctctg gcaggcggcc agccccaatg ccagggcggc
      781 gtgggttttg cccgtaccgg acggcccgag cgcaatgcag ttctggcgct tctcgatcca
      841 ttcgcagcgc gctagttcca gaaccaacgc tttgttgagc gaaggctggg cggtgaagtc
      901 gaaggtgtcg aggcttttgg tctgcggaaa gcgcgccaac cgaatgcggc gctcgacgtt
      961 gcgccgctcg cggtcgatgc gctccagttc gcaaagacgc agcagatagc gcgggtagtc
     1021 ggcgcgatct tgcgctgact ccatcgccac tttctcatac tcgcgcgcga aagtgggaag
     1081 cttcaaagcc ttgagatgat tgcccagcag catctgaggt gcgacggccg tcagcgtcgg
     1141 ggtctcgtgc aacattgtca tgccgggact cctgctgtca ctgggatgga gcctgagctg
     1201 gcaatgagtc cgagataggc gcgcggatct gttgtcccaa ccgtggcggc cggcaagtaa
     1261 gggtagaatg taagatcgag ccgggccggc cggttctcca atctggccag cagcagcatc
     1321 ttcaccgcat cgaagctgat ggcacctagc cgcagcgctt gagcgacggc atgttcgacc
     1381 tgatgctgat ggaagtcctc catcaatcga agcacctgaa tgaactcgcg ccgtccggca
     1441 ttccccatcc gcgcttccat cagccgccgc agacgatgga tgcagtcggc aagctgccag
     1501 ttgtccaggg gcgcagcctg gtcgagcgct ttgctcttgt gttccagcaa cgccaaataa
     1561 tgcaaaggat tgaagacaaa atcggccgta tcatagctgc gggaatgcat ggcgatggtc
     1621 tcgccgcgac aggcgatctc gaccctgtcg acacatccct tcgccagaac ctcttgatgg
     1681 ccgtagcgcg ttggaaccga gtaatcgttg ttcttgtaac gcaccaacga cagagaggag
     1741 acacgggtgg cgaccttgtg acaggcatca tagggcgccg gcggcaacgg catgaacgcc
     1801 gccacgtccg cctgcatacg ctcggcgatc gtggtggtat ggccgcgcaa gatcgcctga
     1861 cgccgttttg tgcaggcatc aagaaaccgc gcattgagcg cctcaaagct ctccgcgaca
     1921 ggaagcggcg tcatgaaatt gcgccggaca taaccaacca gcccctccac cttgcccttg
     1981 tcgttgccct tgcccggcct gccaaaccgg tcgttgaaca ggtaatggct ctgcagttcc
     2041 gcgaacatct gcgatcgcag gcgctgtccg cccttcacga tcttggccac cgccagccga
     2101 gtgttgtcgt acaagataga ctgcggtacg ccgccgaaaa cgtcgaaagc tgctacatgg
     2161 ccgtcgcaga aggcttccgc cgtctcggcc ggatacgcct tgacgaagca gccgtccgag
     2221 tgcggcaagt ctacgcaaag gtaatggaac cggaccttct tgcccgcgat gtaaccatcc
     2281 gcttcgccaa aatctgcctg ggcatggccc gggcgatggc tcaatggcac gaacatctca
     2341 cgggcgcgca gccttgcctg cgccacgtac tcacgcacaa tcgtataccc gccagaaaat
     2401 ccccgttcat cccgcagccg ctcgaaaatc cgatgcgcgg tgtgccgctg cttcttgtga
     2461 acgctgcggt caccttcaag gatggcgtcg atccacgcca tgtccggccc aagcttcttc
     2521 gaagctggcc gttcacgtcg ctggtaaccg ggcggaatcg aaaactgaag catctttgtg
     2581 atcgtgttcc ggtgaacgcc aaactgcttc gctgcctcac ggcggctcat cccatccacc
     2641 atcaccgatc gtctgatctt ggcatagagt tccactgcga acatccttcc agcccctcca
     2701 tgccattcaa tggcatcgag acggcctagc aggaccggta cactttggcg tcgccttcca
     2761 agaggcagat cgaaaccggt tcagtggtac actttgcgcc cgcgatttag aataggcaat
     2821 cgcggaaatg cagcctatcg gttcgtcggc ggattcacca aagaagaatc ccttcggatc
     2881 ggcaacgtag aaacattccc catcatagag gcccggattc cagccctcag cc
//
LOCUS       DATMGX010000249         3831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_101324, whole genome shotgun sequence.
ACCESSION   DATMGX010000249 DATMGX010000000
VERSION     DATMGX010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101324"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..2361)
                     /locus_tag="VNR65_04065"
     CDS             complement(235..2361)
                     /locus_tag="VNR65_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ17886.1"
                     /translation="MSQSKVGTSPVKQGRLTNGDLNLDKARDARGPLEHGIDALSRTS
                     PSLQPPPPLPSSEPPFLAWDDEIDADAQLQLYLDRAKEPKQKGFPPFEALSARLNELP
                     APAFQHTAEDAAIRQPQPSSRRRRASDLEWFEERFLELKTMLSRRESDTSEIVSINVK
                     LAEIIGRVDRLSAALPGEKTMVAVETQLAELSRSLETTRAQSSSDANRIARAAQEILA
                     ATEKAQEARAGFEEAARHTVRELGQTVAVSASRAAAVTAEEIAAALNERCDGGRLDRV
                     ESELRSLNTVSRESCERTTAALERVHQTLRVFLERDQRDRSTAPRRRAGVHTPITAGS
                     HPYAIGDDFGSEPEQKPRLDTITLRKPSPPDPNLLDAFKQASEKLIVSKSPAKTGEGA
                     RAPSARDASRPAFRDEERSLPLFGLGIVAVVLLIASAALYYLHTRTPVPPFHLSVLPD
                     VQPARTLPDLAPFNPRTQDATPGKTVTGAPKAPPSLFTAADQNHEPSPSQPESREDLQ
                     VLANAASRGDREAQFRIAARFLSDGRLQGDPSTAARWLSRAADQGHVESQFVLASLYE
                     RGVGVPKDDTVARELYHKAAIAGHIRAMHNLGVLLSAQDTLQDYQEAAAWFASAAMSG
                     LADSQFNLAVFYERGLGLQKNNQKAYFWYEVASLAGDKEATRQAERLKRQLPDAETQA
                     AAEQAGSWHPSVEQIPHLTSGSASRS"
     gene            2511..2957
                     /locus_tag="VNR65_04070"
     CDS             2511..2957
                     /locus_tag="VNR65_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWJ17887.1"
                     /translation="MSTSRTIAGHKSSTVTFGTAGPGFYRLGGELHRRLTEMGAENGL
                     MTVFCAHTSASLCIQENADPSVLADLGDALKRLAPEDARYRHNSEGPDDMPAHIKTLL
                     TATSLSLPVRAGNLALGIWQEVYLIEHRAGGHKRRLELDFVGTLKG"
     gene            3149..3508
                     /locus_tag="VNR65_04075"
     CDS             3149..3508
                     /locus_tag="VNR65_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ17888.1"
                     /translation="MQTILLAEDDDSMRSFLAKALERAGYEVVSYGNGAEALQGLKEK
                     PVTLLLTDIVMPEMDGIELAKRAAELNPEMKIMFITGFAAVALQHYAESPQRARILSK
                     PIHLKDLVREVDKMLAA"
     gene            complement(3497..>3831)
                     /locus_tag="VNR65_04080"
     CDS             complement(3497..>3831)
                     /locus_tag="VNR65_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWJ17889.1"
                     /translation="KFIIVAEDGKAFGYIVPEQIRRVLQPQWSDTFVRTIAERFTHDT
                     VVSPGASAMEALRKLQSNGIRHLAVLEGNALVGTVSETNFLNYITVREELATPAKALP
                     AEPAPSGS"
BASE COUNT          727 a         1088 c         1203 g          813 t
ORIGIN      
        1 aaggcatcca tgccgtcggc atagtcggca tcgccggatc tggcctttgg gctcatatct
       61 ccgctgaata cggcatgggc aggctgaatg aggccgacgg cgactgtcat gacgattgcg
      121 agcgccgaaa gggcgatcaa caaagtcatt tttcttccaa aggttctttt catgcgagcg
      181 atacgcaatg atccattgga aggatcaatt gccgcaaact gcttctgaaa agaattagct
      241 tcgggaagcg cttccgcttg tgaggtgtgg aatctgctcc acgcttggat gccacgagcc
      301 ggcctgttcg gctgccgcct gcgtctcagc gtccggcaac tgccgcttca ggcgttccgc
      361 ttgacgcgtc gcttccttgt ctcccgccag cgaggcgacc tcgtaccaaa agtaagcctt
      421 ctgattattc ttttgcagtc caagtccgcg ctcgtaaaag acggcgagat tgaactggct
      481 gtcagccagg cccgacatgg cagcgcttgc gaaccaggcg gctgcttcct ggtaatcctg
      541 gagtgtgtct tgagcactaa gcaatacccc aaggttatgc atggcccgga tatgcccggc
      601 gatggcagcc ttgtgataga gctcccgcgc aaccgtgtca tccttgggta ccccaactcc
      661 gcgctcataa agcgaagcca gcacgaattg cgattcgacg tggccctgat cagcggccct
      721 tgacagccag cgcgcggccg tggacgggtc gccctgcagc ctcccgtcgc tcaggaaacg
      781 ggccgcaatt cggaactggg cttcccggtc gccgcggctt gccgcgttgg caagcacctg
      841 caggtcttca cgagactcgg gctgggaagg ggagggctcg tggttctgat ccgctgcggt
      901 gaaaagcgag ggaggagcct tcggagcgcc ggtcaccgtc ttgccgggtg ttgcatcctg
      961 ggttcgcggg ttgaagggag cgagatccgg caaagtcctt gccggctgta cgtccggcag
     1021 tacgctcaag tggaaggggg gaaccggggt cctcgtatgc agataataga gcgcggcgct
     1081 tgcgatcagg agaaccacgg ccacgattcc aagcccgaaa agcggaagag atctctcctc
     1141 gtcgcgaaac gccggcctgg acgcatcccg cgccgatggt gcgcgtgctc cctctccggt
     1201 tttggcagga ctcttagaga cgataagttt ttcgcttgcc tgcttgaacg catccagaag
     1261 gttcggatcg gggggcgagg gcttgcgcag cgtgattgtg tcgagccggg gcttctgctc
     1321 cggctccgat ccgaaatcgt cgccaatcgc gtaaggatga gagccggctg tgatcggcgt
     1381 gtgaacgcca gctcgtctcc ttggcgccgt ggagcggtct ctttggtccc tttcaaggaa
     1441 aacgcgcaag gtttggtgaa cccgctcgag tgccgcggtg gtccgctcgc agctctcacg
     1501 ggaaacggtg ttaagcgagc gaagttcgct ttccacgcgg tcaagacggc cgccgtcgca
     1561 gcgctcattg agggcggctg cgatttcttc tgcggtgacc gcagccgcgc gcgaggctga
     1621 caccgcgacg gtttgcccaa gctccctcac cgtatgccgc gccgcttcct cgaagccagc
     1681 gcgcgcctcc tgggctttct cggttgcggc caatatctcc tgggccgcgc gtgcgatgcg
     1741 gtttgcatcg gacgagcttt gcgcgcgtgt ggtctcaagc gagcgtgaaa gttcggcgag
     1801 ctgcgtttcc acggccacca tggttttctc gccaggcaat gccgcggaaa gccgatcgac
     1861 gcggccgata atctctgcaa gcttgacgtt gatggaaacg atctcgctcg tgtcggactc
     1921 ccggcgcgag agcatcgtct tcagctccag gaacctctcc tcgaaccatt cgagatcgct
     1981 tgcacgccgt ctccttgagg agggctgcgg ctggcgtatg gcggcgtcct ccgccgtgtg
     2041 ctggaaggct ggcgctggga gttcattgag ccgggctgat aaggcctcaa agggagggaa
     2101 acctttctgc ttcggctctt tggcacgatc aaggtaaagt tggagttgcg cgtcggcatc
     2161 tatttcgtcg tcccatgcca aaaaaggagg ctcggacgaa gggagaggcg gaggcggttg
     2221 cagggaaggt gaggtccgag aaagcgcgtc gatcccgtgc tcgagcgggc cccgcgcatc
     2281 gcgagcctta tcgagattta ggtcgccgtt ggtcaagcgc ccttgtttca cggggctggt
     2341 tcccaccttg gattgagaca cgccctcatc cctggtgttt gagcgccggg attgagaatt
     2401 gcgatcgcat agggtgttcg acgacctatg cgattgttat ctttgcctta gatatgcaaa
     2461 catgccgtaa acggccggta aaattatgtg gcttttctta gaggtcgccg atgagcacat
     2521 cccgaaccat agccggtcac aagagctcca ccgtgacctt tggaactgcc ggacctgggt
     2581 tttatcgcct tggaggcgag ttgcaccggc ggttgacgga gatgggggcg gagaacggcc
     2641 tcatgaccgt gttttgcgcc cacacctcgg cctcgctctg catacaggaa aacgcggatc
     2701 cgagcgtgct ggcggatctg ggcgatgctt tgaagcggct ggcgccggaa gacgcgcgtt
     2761 accgccataa cagtgagggg ccggacgaca tgcccgccca cattaaaacg cttctcacgg
     2821 cgacttcgct ttccctgccg gtcagggctg gaaaccttgc gctcggcatc tggcaggagg
     2881 tttacctgat cgagcatcgc gcgggcggcc ataagcgcag gctcgaactt gatttcgtag
     2941 gaactctcaa aggttaacgt atcctgaaag cctaataaga gctggaactt acgcccgctc
     3001 tccgtggggt aaggttaaaa caagcttgag cctggattct tcacaaagat gcgctaaagc
     3061 tgatggggcc acggtggtgc ggtcttgagt ggatgcgtgg caagtctgcc gtccgagact
     3121 tttggtctgt cgcttgcgag ttagagaaat gcaaaccatt ctgcttgccg aagatgatga
     3181 ctcgatgcgc agcttcctcg cgaaggcgct cgaacgtgcc ggctatgaag tcgtgtccta
     3241 cgggaatggc gccgaggccc ttcaagggct gaaggaaaag cccgtcacgc tgttgctgac
     3301 cgatatcgtg atgccggaaa tggatggcat cgagcttgcc aagagagcgg cggaactcaa
     3361 tcccgagatg aaaatcatgt tcatcacggg ctttgccgct gtcgcgttgc agcactatgc
     3421 ggagtcacca cagcgggcgc gcatcctttc gaagccgatc catctgaagg atcttgtccg
     3481 cgaagtcgac aagatgctag ctgcctgagg gcgccggctc tgccggcagc gcttttgctg
     3541 gagtggcgag ttcttctcgc accgtgatgt aattcaagaa gttggtctcg gacacggttc
     3601 cgacgagcgc attgccttcc aggacggcga ggtgacgtat tccgttcgac tgaagcttgc
     3661 gcagcgcttc catagcggat gccccgggtg agactaccgt atcgtgggtg aagcgctcgg
     3721 ctatcgtccg cacaaaggta tcgctccact gcggctgcaa aacgcgccgg atctgttcgg
     3781 gcacgatata gccgaaagct ttcccgtcct cagcgacgat gatgaatttg c
//
LOCUS       DATMGX010000250         4462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_48082, whole genome shotgun sequence.
ACCESSION   DATMGX010000250 DATMGX010000000
VERSION     DATMGX010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48082"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..3706
                     /locus_tag="VNR65_04085"
     CDS             41..3706
                     /locus_tag="VNR65_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HWJ17890.1"
                     /translation="MTKKEKGPAKTPASPAKGRLSAAGKSSGAASRPQFPVVGIGASA
                     GGLEACTALLKALPVNPGMAFVVVQHLDPHHESILHKLLSKTTEMPVMQVEDGMVLEP
                     NHVYVIPPKYDMAVRERTLRLLSRRRTAGRHLPINRFFVSLAEDQKGAAIGVILSGTA
                     SDGTVGLQAIKSEGGVAFAQDPKSAVYPGMPESAILAGYVDFVLPPEEIARELIRLEH
                     LPYAKRTAAVLESEPPLVGAADLQKIIQILRTATGVDLSLYKTGTIKRRVARRMILRK
                     IDSLERYGSYLEENVVEVAALYQDIFIHVTSFFREPETFATLQRKVLPKLMAKRPQGE
                     PLRIWVPGCSTGEEVYSIAITLMEFLDKRASRAAIQIFGTDISAPAVDTARAGTYSET
                     AAANVSPERLKRFFVKVGDGYQIGKQVRDLCVFARHDLGKDPPFSQLDLISCRNVLIY
                     MSAALQERVFSFFHYALKPAGFLLLGKSEALGSSAHLFVQVESKRKIYSRNPAVAVAR
                     SALAPTDHVKAAARAATAVTDAAFDLEKAVERIIRQRYAPAALVVDAGLQVLHFEGDT
                     GPYLAPARGAATLNVLKLVRKELALDLHTALHRAKKEETPVRREGIRVPQDGGSKTVN
                     LEVIPMKGRHAKGTDFLILIEEVPAREAAEPREAREEDELVHLKRELSSTREHLQSII
                     ESQEATNEALTVEHEEVLSTNEELQSTNEELETAQEELQSSNEELSTLNEELQNRNAE
                     LGQLANDLSNLLVGVSIPVIIVGGDRRIRRFTPAAEPLLNLLPADVGRPISDIRPNID
                     VPDLDSLISEVSGKGSFVEREVQGRDGHWYSLRMRPYRTAENKIDGVLMALMDIDVMK
                     RGLDQTLRSLDDAVAERDLSASLLDMSGALTVILDPTGRIVAFNHSCQEKSGYSLREA
                     KGRTFWDFLLPPEEVDEAKAAFAGILECAEPARNYERTWIGKDGSRRVIASSSIGRCG
                     ADGAVRQIISTGIDITPRKLAQDALHRSEDQLRRLTANLITAQEEERKRVARELHDDV
                     NQRMAMLANEVATLERTLPGSDRLLRKQLRSLREQVDQLSDDLRRTAQHLHPSALEHF
                     GLVTALESHCSDFLKRHQIQLKLTHRSVPESIPVEVSLCLYRVTQECLHNIAKHSGAR
                     QVAVEIRGNEEGVLLSIADNGTGFDPGLVADQSGLGLVGIRERVRLVDGIVSINSQPG
                     HGTKIDVRVPLTKRMAP"
     gene            3703..4344
                     /locus_tag="VNR65_04090"
     CDS             3703..4344
                     /locus_tag="VNR65_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ17891.1"
                     /translation="MKRPRVLLADDHTLVVEGLRRILEPECEVVGAVEDGRSLLVAAE
                     QLTPDIILLDISMPLLNGVDAARRLRDTVPSAKVIFVTMHADATYVAGAFRAGAAGYV
                     LKRCASMELLNAVHEVLRGRTYVTPLIRKDILGELPGWPLGSGETSGELTDRQREVVQ
                     LVAEGHHVKEIAAILNISSKTVAFHKSNVMRRLGIRSTAELTKYALDHGITGS"
BASE COUNT          964 a         1306 c         1368 g          824 t
ORIGIN      
        1 ggctgacgca gccaggaatg acctggcagc ggaggagtac gtgacgaaaa aagagaaggg
       61 accggcgaaa actccggcgt cacccgcgaa aggccggctg agcgctgcag gaaaatcctc
      121 cggcgcagcc tccagacccc aatttccggt tgtggggatt ggcgcctccg ccgggggcct
      181 ggaggcttgc acggcgttgc tcaaggccct gccggtcaac cccgggatgg cgttcgtggt
      241 ggtccagcac cttgatcctc accacgagag cattctgcac aagctactct ccaagacaac
      301 ggagatgcct gtcatgcagg tggaagacgg aatggtcctg gaaccgaatc atgtttacgt
      361 gatccctccg aagtacgata tggccgttcg cgagaggacg ttgcggctcc tatcccgccg
      421 gcggaccgcc ggcagacacc tgccgattaa tcgcttcttc gtttccctcg cggaggacca
      481 gaagggtgcc gccatcgggg ttatcctatc gggaacggca tccgacggca ctgtgggatt
      541 gcaggccatc aagtccgaag gcggcgttgc ctttgcgcag gaccccaagt cggccgtcta
      601 tccgggcatg ccggagagcg cgattctggc ggggtacgtg gattttgttt taccgcccga
      661 ggaaattgcc cgcgagctta tccggctgga gcatttacct tacgccaaaa gaaccgccgc
      721 cgtgctggag tcagaaccgc cgttggtggg cgcagccgat cttcaaaaaa tcattcagat
      781 tctgcggaca gccactgggg tcgacctcag cctttacaaa acgggaacga tcaagcgacg
      841 tgttgcacgc cggatgatcc tccgcaaaat cgacagcctc gaacgttatg gatcgtacct
      901 tgaggagaac gttgtcgagg tagcggcgct ctaccaggac atttttattc acgtaaccag
      961 ctttttccgg gaaccggaga cgttcgcgac actgcaacga aaggtcctcc caaagctgat
     1021 ggcgaaacgg ccgcaagggg aaccgcttcg catatgggtt cccggatgct ccacgggcga
     1081 ggaggtgtac tcgattgcga taacgcttat ggagtttctg gacaaacggg cctccagggc
     1141 cgctatccag attttcggca ccgacatcag tgcgccggct gttgacacgg cacgggccgg
     1201 cacctactcg gaaactgccg cagccaatgt ctccccggag cgtcttaagc gtttctttgt
     1261 aaaagtggga gatggctacc agatcggtaa gcaggtccgt gacctgtgcg tgtttgcccg
     1321 gcatgacctg ggcaaagacc cgcctttctc ccagctcgat ctcatcagtt gccgcaatgt
     1381 attgatctac atgagtgcgg cgcttcaaga gcgggtgttt tccttcttcc attacgccct
     1441 gaagccggcc ggctttcttt tgctgggaaa atcagaggcc ctcggttcct cagctcatct
     1501 ctttgtccag gtggagagca agcgtaagat ctattcgcgc aacccggcgg tggccgtggc
     1561 tagatctgcc ctcgcgccca ctgatcacgt aaaagcggca gcgcgcgccg ccacggcggt
     1621 gacggacgcc gcgttcgacc tggagaaggc cgtcgagcgc atcatccggc agcgatacgc
     1681 cccggcagcg ctggtggtag acgcgggctt gcaggtcctc cacttcgagg gggacaccgg
     1741 accatacctg gcgccggcgc gaggagctgc aacccttaac gtgttgaaat tggtgcgcaa
     1801 agaactggcg ctggatctcc acacggcact tcaccgggcg aaaaaggagg agaccccggt
     1861 tcgcagggaa ggtattcgcg ttccccagga cggcggatcg aaaacagtca atctggaagt
     1921 catacccatg aagggccgcc acgcgaaggg gacggatttc ctcatattga tcgaggaggt
     1981 tccggcgcgc gaagcggcgg aaccgcgcga agccagggag gaagacgaac tcgtccattt
     2041 gaaacgggaa ctgtcttcca ccagggagca tttgcagtcc atcatcgaga gccaggaagc
     2101 cacaaacgag gcgctgacgg tggaacatga ggaggtgctg tcgaccaacg aggaattgca
     2161 gagcaccaac gaagaactgg agactgccca ggaggaactg caatcctcga acgaggaatt
     2221 gagcacgctg aacgaggagt tgcagaaccg gaacgcagaa ctgggccaac tcgccaacga
     2281 cctgagcaat ctgcttgtcg gcgtcagcat cccggttatc atcgtgggag gcgaccgtcg
     2341 aatccggcgc ttcactcctg cggcggaacc gttgctgaac ctgcttcccg ccgacgtggg
     2401 ccgtccaatc agcgatatca ggcccaacat tgatgtgccc gacctggaca gcctgatctc
     2461 ggaagtgagc ggtaaaggca gctttgttga acgagaggtc cagggccggg acggacattg
     2521 gtattccctc cggatgagac cctacaggac cgccgagaac aagatcgatg gggtgctgat
     2581 ggcgctcatg gatatcgacg tgatgaagcg cggcctcgat cagaccctgc ggtcccttga
     2641 tgacgcagtc gccgagaggg acctgtcggc cagcctcctg gacatgagcg gcgccctgac
     2701 cgtcattctc gaccccactg gccgcattgt cgccttcaac cactcctgcc aggagaagtc
     2761 gggctactcc ttgcgtgagg caaaaggccg gacgttttgg gacttcctgc ttccaccgga
     2821 agaagtggac gaggcgaaag ccgcgtttgc gggcatcctc gaatgcgcgg agcccgcgag
     2881 aaattacgaa agaacctgga tcggaaagga cggctcccgc agggtgatcg cctcgtcaag
     2941 catcgggcgt tgtggcgcgg atggcgctgt caggcaaatc atcagcaccg gtatcgacat
     3001 caccccacgc aagctcgccc aggatgccct acaccgcagc gaagaccaac tccggagatt
     3061 gacggcgaac ctcattaccg ctcaggaaga ggagcgcaaa cgcgtggcgc gagaactcca
     3121 tgacgacgtg aaccagagaa tggccatgct ggcaaacgag gtggccacat tggaacggac
     3181 tcttcctggt tccgaccgcc tcctccgcaa acagctccga tcgcttcgcg agcaggtgga
     3241 tcaactctca gatgacctcc gccgtacggc tcaacatctt catccttcgg cccttgagca
     3301 tttcggcctg gtgacggcac ttgagtccca ctgttccgac ttcttgaaac ggcaccagat
     3361 ccagctcaag cttacgcatc gcagcgtgcc ggagtccatt cccgtcgaag tctcgctgtg
     3421 cctctatcgg gtcacgcaag aatgtctgca caatattgcg aagcattccg gagccaggca
     3481 ggtagcggtg gagattagag ggaacgaaga gggcgtcctc ttgtctatcg ccgataacgg
     3541 cacggggttc gatcccggct tggtggcgga ccaaagcggg ctgggcctag tcggcattcg
     3601 ggaacgagta cggcttgtgg atgggatcgt ttcgatcaac tcgcagccag gtcacggaac
     3661 gaagatcgat gttcgagtcc ccctgacaaa gaggatggcc ccatgaagcg accgcgcgtg
     3721 ctgcttgcgg atgaccacac gctggtcgtt gaagggcttc gaaggatact ggaacccgag
     3781 tgtgaagtcg tgggagcggt ggaagacggc cggtcactac tggtggccgc ggaacaactg
     3841 acgccggaca tcatcctgct cgatatctcc atgcccctgt tgaatggcgt ggacgccgcc
     3901 cgcaggctac gcgacaccgt gcccagtgcc aaggtgatct ttgttaccat gcacgcggac
     3961 gcaacttacg tcgctggggc cttccgcgcc ggagcagccg gctatgtctt gaagcggtgc
     4021 gcctcgatgg agttgctgaa cgccgtccac gaggttctta ggggtcggac ctacgtcacg
     4081 cccttgatca ggaaagacat cttgggcgaa ttgccggggt ggccgcttgg gtctggggag
     4141 acctccggcg aactaacgga ccgccagcgg gaagtggtgc aactggtggc ggaaggacat
     4201 cacgtcaaag aaattgcggc aatcctcaac atctccagca aaacggtggc attccacaag
     4261 tcgaacgtga tgcggaggct gggcattcgc agcaccgccg aattgacgaa gtacgctctg
     4321 gaccacggca tcacgggatc ctgaccagcc ttggcagcag ggccaatccc aggcatggct
     4381 ctttctagcc atgactggcc tggacatttg cccagttccc acggctccta ccgaaggcta
     4441 acaatcgatg tagcgcatag cg
//
LOCUS       DATMGX010000251         2571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_44662, whole genome shotgun sequence.
ACCESSION   DATMGX010000251 DATMGX010000000
VERSION     DATMGX010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44662"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VNR65_04095"
     CDS             <1..349
                     /locus_tag="VNR65_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17892.1"
                     /translation="QLEEISIGDYVLSGGEIAAMVLIDACVRLLPGVIGAEESLSEES
                     FENNLLEYPHYTRPRVWEGREIPDVLLSGDHARIARWRREQAEQLTRQRRPDLLEGKG
                     RATEPALPPKESA"
     gene            complement(312..941)
                     /locus_tag="VNR65_04100"
     CDS             complement(312..941)
                     /locus_tag="VNR65_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17893.1"
                     /translation="MALANLENRLHFNGGKRFRVTGYACRGVVMRIFPLSIVFSLIFL
                     VKPAHSEASDLFHEKISIIASKFSALNIPGLEKIQLPQIQPDGSFIFIYVWHPQTHRP
                     TTASFEANIGDYFDIVGAQAKAFRGWCYVSKPETDVLKAETGEIAVIRIWALGFHGHV
                     PRRKCRGGLPGEAGKLGQTQVIKDPRSGLLSMVRSGHPTRFPSAAERVQ"
     gene            1107..1352
                     /locus_tag="VNR65_04105"
     CDS             1107..1352
                     /locus_tag="VNR65_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17894.1"
                     /translation="MVTHEEVDEFFRRLLAGIPARAERRPDPVDQSRQLLTAFNKIRD
                     PRLRQELLVLIEIVSQKPELLQAKREHWAKGTLAPMH"
     gene            complement(1370..1831)
                     /locus_tag="VNR65_04110"
     CDS             complement(1370..1831)
                     /locus_tag="VNR65_04110"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWJ17895.1"
                     /translation="MIRDKISQTLKEAMKAKDEAKTTTLRTVNAAIKQKDIDVARGRG
                     DQHISEEEVLNLLQSLIKSRRESIALYQQGGRQDLVDKESAEIAMIEQFLPKQMSEEE
                     TRAAIRELVASLGASSVKDMGKVMAALKSTYAGQLDMTKASAAVKDVLTGK"
     gene            1985..2401
                     /locus_tag="VNR65_04115"
     CDS             1985..2401
                     /locus_tag="VNR65_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ17896.1"
                     /translation="MLNRTKALIVAAAAILPQWAYAEEGAPGGCEPLAEISGATLFAE
                     NCTLCHGPDGKGGGPLALAKNLTPPDLTTLAARTHGKFPMDHVSDELRHGGGEKADGD
                     KSMPVWKKIFAHECGDAYARQAEIELENFLRMIQEK"
BASE COUNT          572 a          721 c          703 g          575 t
ORIGIN      
        1 gcaactcgaa gaaatctcca tcggcgacta tgtgctgtcg ggcggcgaga ttgccgcgat
       61 ggtcttgatc gacgcctgtg tgcgcctgtt gcccggcgtt atcggcgccg aggagtcgct
      121 cagcgaggaa agcttcgaaa ataatctgct cgaatatccc cactatacga ggccgcgcgt
      181 gtgggaagga cgcgaaattc cggacgttct gttatccggg gatcacgccc gcatagcacg
      241 ctggcggcgt gagcaggctg aacaattgac gaggcagcgc cgccccgatt tgctggaagg
      301 aaaaggccgc gctactgaac ccgctctgcc gccgaaggaa agcgcgtagg gtgccctgat
      361 cgcaccatcg acagaaggcc tgatcgcggg tctttgatga cttgcgtctg gccgagcttc
      421 cccgcctcgc ccggcaatcc gccccggcat ttgcgacgtg gaacatgacc atggaagcct
      481 aaagcccaga tccgaatgac ggcgatctcg ccggtttcag ccttcagcac gtccgtttcg
      541 ggtttgctaa cataacacca ccccctgaac gccttggcct gcgcgcccac gatgtcgaaa
      601 tagtcgccga tattggcttc gaacgaagcc gtggtcgggc gatgtgtctg cggatgccac
      661 acgtagataa atataaacga accgtccggc tggatttgag gaagttgaat cttctcgagg
      721 cctggaatat tcagcgctga aaactttgaa gcgataatac tgatcttctc atgaaacaga
      781 tctgacgctt ccgaatgtgc aggcttaacc aagaagatca acgaaaacac aatcgaaaga
      841 ggaaaaatcc tcatcactac ccctcggcaa gcgtagccgg tgacgcgaaa ccttttccca
      901 ccattgaagt gtaaacgatt ttctaggttt gccaaggcca tttctcattg cttgaaccgg
      961 cctattggga tacttataat tggacgtact ttcgcttttg aatgtccgaa gttttctttt
     1021 tttgtatgta gtttttcact taaaattgca aatgttcggt tatacaaccc taagtgctgc
     1081 gtggcgttga gtgtgaggcg tttgtgatgg ttacgcatga agaggtcgac gaatttttcc
     1141 gacgcctgct cgcgggcata ccggcgcggg ctgaacgcag gccggacccc gtcgatcaat
     1201 ccaggcagct cctgacagcc ttcaacaaga tcagggatcc tcgactgcgg caagagcttc
     1261 tggtcctgat cgaaatcgtc tcgcagaaac ctgagctctt gcaagccaag cgcgagcatt
     1321 gggcgaaggg cacgctcgcg cccatgcact gaggcggtca cgccgattgt cacttgccgg
     1381 ttagcacgtc cttcacggcc gcgctggctt tggtcatgtc aagctgcccg gcatacgtgg
     1441 atttaagcgc ggccatgacc ttccccatat ccttcacgct gctcgcgccc agcgaggcga
     1501 ccaattcgcg aatggcagcc cgtgtctctt cctcgctcat ttgctttggg agaaattgct
     1561 cgatcatggc gatttcggca gattccttgt caacgaggtc ctgtcttccg ccctgctgat
     1621 aaagcgcgat ggattcacgg cgcgacttga tcaagctctg caagagattc agcacttcct
     1681 cttcactgat gtgctggtcg ccgcggccgc gcgccacgtc gatgtccttc tgcttgatcg
     1741 ccgcattcac cgtgcgcagt gttgtggtct tggcctcgtc cttggccttc atggcctcct
     1801 tcaaggtttg tgagatcttg tcgcggatca tggctccatc cctttccttt ggcgcatgat
     1861 gtagcgcttc cgaagctcag ccacaaactc ataaagcaag aggataagcg gcgccgtcat
     1921 ttggcgtcgg aaacgaatcg atgcgtcctt gagcttgctg aacagtcatt caggagcagg
     1981 gccaatgttg aaccgaacga aagccttgat cgtagctgcc gctgccatcc tcccccaatg
     2041 ggcatacgcc gaggaaggcg cgcctggcgg ttgcgagccg cttgccgaaa tctcaggtgc
     2101 tacacttttt gccgaaaatt gcacgctctg ccacggccct gacggaaagg gcggcggtcc
     2161 cttggcgctc gcgaaaaatc ttacgccgcc ggatctgacg acactggcag cgcgaacgca
     2221 tggcaaattt cccatggacc atgtttccga cgaactccgg catggcggcg gggaaaaagc
     2281 cgatggcgac aagagcatgc cggtttggaa gaaaatcttc gcacatgaat gcggagacgc
     2341 ctatgcgcgc caagccgaaa tcgaactcga aaactttcta aggatgatcc aggaaaaata
     2401 gacggcgtcg ccggagccac agagaaatat ttagtcgatg caatagctgc gcggatgagc
     2461 ccattatatt gactgccggt gtttttcccc ggcgacaagg cttagtattc gctttggcgg
     2521 cagcttggat ttgcgaaaac acaaacaacg gacgaaaaag ccaataacct c
//
LOCUS       DATMGX010000252         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_299419, whole genome shotgun sequence.
ACCESSION   DATMGX010000252 DATMGX010000000
VERSION     DATMGX010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299419"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VNR65_04120"
     CDS             <1..1069
                     /locus_tag="VNR65_04120"
                     /inference="COORDINATES: protein motif:HMM:NF021642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="virulence factor SrfC family protein"
                     /protein_id="HWJ17897.1"
                     /translation="RNPNYKAEGIIEYEGRKEVRIHADKQERIAKLRAAYAHIREVND
                     HFRDPLKAFDEVMKLNDGGISYLAANLADVCRPGMKLDQIRVRLKDSRRALAGLLAPK
                     YVPTDLDKRLAERELIADKILEEFGACLQKNAFGTFLRGLCVERGALADALHEPSQAI
                     QTAVRAKEPGNLISIVKSNGNSSGKIAAAPSPSRVDRLARTSIQVWTQNLHDVSNDEV
                     FSSRIGVSASSLRELANELVATSRRLEVEAEIRHTLDKIAHLERPHEIWAKATIVAER
                     VLNRFVTTLGSAGPEHPVLFDAAGMDQLQMSFQEEFAVSWLRSFYAHLKENAQTADGL
                     VHDAEQNLILGKLLNALNTEN"
     gene            complement(1589..2569)
                     /gene="dmeF"
                     /locus_tag="VNR65_04125"
     CDS             complement(1589..2569)
                     /gene="dmeF"
                     /locus_tag="VNR65_04125"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HWJ17898.1"
                     /translation="MHSHSLESFQHEHVFLGERHDQNAKRVWIVVGLTAVMMVGEIVG
                     GTVFGSLALVADGWHMSTHAAALTISALAYVYARRHLHDERFAFGTGKLGDLASFASA
                     IVLGMVAFLIGYESVQRLMNPVAIWYDEAIAIAVLGLGVNLASAWLLRDDHNHHHGHD
                     AHADHDDHDGHDHHLHDHHHDRDLNLRAAYVHVLADALTSVLAIAGLVTAKLAGWTFM
                     DPVVGLVGTGVILSWAYGLLRQSGAVLIDAVPDQGLVERIRNELEVDGDAISDLHLWR
                     VGPGHYAAVISLVTHDPKRPETYKGKLESLPKLSHVTIEVQRCPEHAFSE"
BASE COUNT          620 a          788 c          801 g          533 t
ORIGIN      
        1 cagaaatcca aattataagg ctgaggggat cattgaatac gaaggacgca aagaagtccg
       61 catccatgca gacaaacaag agcggattgc aaagctccgc gccgcctacg cccacatccg
      121 ggaggtcaat gaccatttcc gggatccatt aaaggcattc gacgaggtta tgaaactcaa
      181 tgacggcggc atctcctacc ttgccgccaa cctcgccgat gtgtgccgcc ccggcatgaa
      241 gctcgatcag atccgcgtcc ggttaaagga ttcccggcgc gctcttgcgg ggctgcttgc
      301 gcccaaatat gtgcccaccg atctcgacaa gcggctggcg gagcgggaac tcatcgcgga
      361 caagatcctg gaagagtttg gagcctgctt gcagaaaaac gcgttcggta ccttccttcg
      421 cgggctatgc gtcgaacgcg gagcgttagc cgacgccttg catgaaccga gccaagcgat
      481 ccagacggca gtccgcgcca aggaaccggg caatctcatc agcatcgtaa agtctaatgg
      541 aaatagttcc ggcaaaatcg ccgcggcgcc ctcgccttcg cgcgtggacc ggcttgcgcg
      601 cacctctatc caggtttgga cgcagaacct tcacgacgtg agcaacgatg aagtcttttc
      661 aagccggatc ggtgtttctg caagctcgct ccgcgaattg gccaatgaac tcgtggccac
      721 ttcccggcgc cttgaggttg aggcggagat ccggcatacc ctcgacaaga ttgctcacct
      781 tgagcggcct cacgagatct gggcaaaggc aacaatcgtc gcggaacgcg tcctcaacag
      841 gttcgtaacc acgctcggca gcgcaggacc cgagcatccg gtgttgttcg acgccgccgg
      901 aatggaccaa ctgcaaatga gtttccagga agagtttgcg gtttcctggc ttcggtcttt
      961 ctacgcgcac ctcaaagaaa acgctcaaac agcagacgga cttgtgcacg atgcggagca
     1021 gaacctcatt ctaggaaagc tccttaatgc attgaacacc gagaattgag gtttttaggc
     1081 caggtcgatt catgcgcgtt gggagtatgg cagtcgatga gttcggccgg gcgaaaaatg
     1141 ggttccacaa tcttcccgtc agctctaatg agtgaacggc acgggcagcg gcatttttcg
     1201 gttatggcac agcgtcgatg agaacagctc ctgtcttgcg tagaaggcca tgggcccagc
     1261 tcaggccgac gatagaggcc acgaacgtcc tgagcccggc gatcgccacc aacacagaag
     1321 tcacggcgtc ggcgaggaca tgacataggc cgcacggaga ttgaggtccc ggttgtagtg
     1381 atgatcgtga tgatagtggt cgtggcgatg atcgtcgcac agtagccatg cttgcgaagt
     1441 tcacgcccag gccaaggaca gcgatggccg atatagcctc tcatagccga tgagtcttac
     1501 ggagtacggg cctcgcagtg tgatgatcgg taatatcgcg gagcagataa acgagagacg
     1561 cggtctgaac gctttcttcc gcaatttact attcggaaaa tgcgtgttcg gggcagcgct
     1621 ggacttcgat cgtgacgtga ctgagcttcg gcaaagactc gagctttcct ttgtaagtct
     1681 ccgggcgctt aggatcgtgc gtcacaagtg aaatcacggc ggcgtagtgg cctggaccca
     1741 cccgccaaag atgcaggtcc gagatcgcat ctccgtcgac ttcaagctca ttgcgaatcc
     1801 gttccaccaa tccttgatcg ggcactgcgt ctatgagaac ggctcccgat tggcgcagaa
     1861 ggccataggc ccagctcaga attacgcctg taccgacgag gccgacgacg gggtccatga
     1921 acgtccagcc cgccagcttt gctgtgacga gtccggcgat cgccaacaca gaggtcaggg
     1981 cgtcggcaag gacatgaaca taggccgcgc ggagattgag gtcgcggtcg tggtgatgat
     2041 cgtgaagatg gtggtcatgg ccatcatggt catcgtgatc ggcatgcgcg tcatgcccat
     2101 gatgatggtt atgatcgtcg cgtaggagcc aggcgcttgc gaggttcacg cccaggccaa
     2161 ggacagcgat ggcgatggct tcgtcatacc aaatggcgac cgggttcatg aggcgctgca
     2221 cgctctcata gccgatcaga aacgcgacca tgccgagcac aatggcgctt gcaaaactcg
     2281 caaggtcgcc aagcttgccc gttccgaagg cgaagcgctc atcgtgaaga tgacgccgcg
     2341 cgtacacgta ggcgagggcc gatatcgtga gcgctgcggc atgcgtcgac atatgccagc
     2401 catccgccac cagggcgagt gagccaaaca ccgtgccgcc aacgatctcg ccaaccatca
     2461 tgacggcagt caggcccaca actatccaaa cgcgcttcgc attctggtcg tggcgctcgc
     2521 ccaggaacac atgctcgtgc tggaatgatt caagtgaatg actgtgcatc ttcacaaact
     2581 ccgcctcacg cagaaggtgg tacctcggcc caaagatcgt agtcgtccga atcgatgatc
     2641 gttgctcgga cgcggtcgcc gggcttcaga tcctctgcct cttcaatgaa aaccgagccg
     2701 tcgatgtccg gcgcgtccca tacgctccgt ccggttgcgc cg
//
LOCUS       DATMGX010000253         2866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_85333, whole genome shotgun sequence.
ACCESSION   DATMGX010000253 DATMGX010000000
VERSION     DATMGX010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85333"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="VNR65_04130"
     CDS             complement(<1..1337)
                     /locus_tag="VNR65_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HWJ17899.1"
                     /translation="MMPGLLTSLSFTSPWMLAGLAALPVIWWLLRLTPPRPESVAFPP
                     TSLLLGLKSREKTPVRSPWWLTALRMLVAAAVIGALAGPVIKHTAKASAALAQPLLVV
                     VDNGWAAGSRWAARQAFAEQLASLADDNGQTLFLAPTAGPASPLQPLTPAEFRQHFAS
                     LTPQPYPGDRATLAQQIEKELSSRRGKLRVAWLGDGVDDAGADALGKTLGALAADGTI
                     DIYGDASGAGPLALTQPSSSAAGAIHARILKADPGPRAGLVAAMTARGDRLAAVPFDI
                     AGANTLADVSLDLPLDLRNQIARLEIVGESSTGGVYLLDGRSQRRRVGLVASEGTDEA
                     QPLLSPTYYLERALTPFAEVTKAQTANLDQATTDLTNASPSVMILSDVGRISGNVAER
                     LRGFVEKGGMLIRFAGPRLEQGGDALLPAPLREGGRTLGGAMSWASPQTPAAF"
     gene            complement(1334..2287)
                     /locus_tag="VNR65_04135"
     CDS             complement(1334..2287)
                     /locus_tag="VNR65_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWJ17900.1"
                     /translation="MSSNLLSRPSALALEGASQDLVGKLPALLLEARRVAVTVAQGTH
                     GRRRAGPGDTFWQYRNFEVGDSSSLIDWRRSASSSHLYVREREWEAAHTVWIWIDLSP
                     SMHFRSALSQTSKAERAVVLALALATLLSDAGERVGIPTVMEPKARQNTATVMMETLA
                     RSLSSLEGLPNATNRLSRHSEMVIFSDFLEPESTLAKVFNKFAVQGVRGHLMQILDPA
                     EESLPYQGRVEFVDAENGARYVAERAETLRAEYRKKLAAHKAALALHAANVGWTPFLH
                     HTDHPAVEALLPLHMHLSGLEKAYRAKGLIKGQTAARNGLS"
     gene            complement(2307..>2866)
                     /locus_tag="VNR65_04140"
     CDS             complement(2307..>2866)
                     /locus_tag="VNR65_04140"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWJ17901.1"
                     /translation="AGQTYALPSPFFVLATQNPLEQEGTYPLPEAQLDRFLLQVDVTY
                     PDAAAERRMLYSTTGLEDRTVTQVLNAEEVMAAQRLVRQLPTGDSVVDAILKLVRSAR
                     PQTAEAAARVDELVAWGPGPRASQALMLTVRARALLEGRFAPSVDDVIALAKPVLRHR
                     MALTFTARTENITVDQVIDGLLAGL"
BASE COUNT          557 a          883 c          924 g          502 t
ORIGIN      
        1 tcaaaggcag caggcgtttg cggcgacgcc caggacatag cccccccgag cgtgcgtccg
       61 ccttcgcgca agggtgcggg cagcaacgca tcgccgccct gttccagtct tggccccgcg
      121 aagcggatca acatgcctcc cttttccacg aagccgcgaa gccgttcggc cacgttgcca
      181 gagatgcgcc caacgtcgct gagaatcatg acggacggcg aggcatttgt aaggtctgtt
      241 gtggcctgat cgagattggc ggtttgggct ttcgtgacct ccgcgaaggg cgtcaaggcg
      301 cgttcgagat agtaggtcgg cgagagaagc ggctgggctt catccgtgcc ttcgcttgca
      361 accagaccga cgcgccgccg ctgcgaccgc ccgtcaagaa gatagacgcc gcctgtcgag
      421 gattcgccca cgatttcaag tcgggcaatc tggtttcgaa ggtcaagggg aagatcgaga
      481 gacacgtctg caagcgtgtt cgcgcctgcg atatcgaaag gaacggcagc cagcctgtcg
      541 ccgcgcgcag tcatggcagc gacaagcccg gctcgcggcc ccggatctgc ttttaagatc
      601 cgtgcgtgga tcgcaccagc ggcactcgac gatggttgcg tgagggcaag cggtcccgcc
      661 cccgacgcgt cgccgtagat atcgatggtc ccgtccgcag caagcgcgcc gagggtcttg
      721 cccagcgcgt cggctcctgc atcgtccacg ccgtcgccaa gccaagcgac gcgaagtttg
      781 ccgcgacgcg agctgagttc cttctcgatt tgctgagcaa gggtagcgcg gtcgcctgga
      841 tagggttgcg gagtaagcga cgcgaaatgc tgccggaact ctgcgggcgt gagcggttga
      901 agcggagacg ccggtccggc cgttggagcg agaaagagcg tctggccgtt atcgtcagcc
      961 aagcttgcga gctgttcggc gaatgcctgc cgggcggccc accggcttcc tgctgcccaa
     1021 ccattatcga cgacaacaag aagcggttgt gccagcgccg ccgatgcttt tgccgtatgc
     1081 ttgatgacag ggcctgccaa tgcgccgatc acggccgcgg cgaccagcat gcggagggcc
     1141 gtgagccacc aggggcttct gacgggagtt ttttcgcgcg atttgagtcc gagaagaagc
     1201 gaagtcggcg gaaacgccac ggactcgggc cgcggtggcg tcaggcgaag gagccaccag
     1261 atcaccggca atgcagccag ccccgcaagc atccaaggcg aggtgaagct gagagaagtc
     1321 agaaggcctg gcatcatgac aagccattcc ttgctgccgt ttggcccttg ataaggccct
     1381 tggcgcgata ggctttttca agcccggaga gatgcatatg gagaggcagg agggcctcga
     1441 cggcgggatg gtcagtgtga tggaggaagg gcgtccaccc gacgttggct gcgtgcagcg
     1501 caagagcggc cttatgggcg gcaagcttct tccggtattc ggccctcagc gtctcggcgc
     1561 gctcggcaac ataccgggcg ccgttctcgg cgtcgacgaa ctccacgcgg ccctgatagg
     1621 gaaggctttc ctccgccgga tcgagaattt gcatgagatg gccgcggacg ccttgcacgg
     1681 caaacttatt gaagaccttt gccaaagttg attccggttc gagaaaatcg ctgaaaataa
     1741 ccatttcgga gtgacggctc agccggttcg tcgcattcgg caacccctct aaactcgaga
     1801 gcgaacgcgc gagcgtctcc atcatcaccg tggcggtatt ttgccgcgcc ttcggctcca
     1861 tgacggtggg tatgccgacg cgttcccccg cgtctgacag aagggtcgcc agagcgagcg
     1921 ccagaacgac cgcgcgctcg gctttcgagg tctgtgacag ggccgagcgg aaatgcatcg
     1981 acggcgacag gtcgatccaa atccagaccg tgtgggcggc ctcccattcg cgttctcgca
     2041 cgtatagatg gcttgaactc gccgaacgcc gccaatcgat gagcgaagat gagtcaccga
     2101 cctcgaaatt ccgatactgc cagaatgtat cgcccgggcc ggcacgccgt ctcccatgcg
     2161 taccctgagc cacggtcacg gcgacccgac gcgcctccaa tagcagcgcg ggcagctttc
     2221 ccaccaaatc ctgggaagcg ccctcaaggg caagagccga tggcctggag aggagattcg
     2281 atgacatcaa gcgtgctgcg gcctcatcac aagccagcca ggagcccgtc gatcacctga
     2341 tcgacggtta tgttttctgt gcgcgccgta aatgtcagcg ccatgcgatg ccgcagaacc
     2401 ggctttgcca aggcgatcac atcgtcgacc gaaggggcga agcgtccctc aagcagcgcc
     2461 cgggcgcgca ccgtcagcat gagcgcttgg ctcgcacgcg gacccggccc ccatgctact
     2521 agctcgtcca cgcgcgcagc ggcttccgcg gtttgaggac gcgcggagcg aactagcttg
     2581 aggatcgcat cgacgacgct gtcgcccgtc ggaagctgcc gcacgaggcg ctgagcagcc
     2641 ataacttcct ccgcgttaag gacctgagtc acggtcctgt cctcgaggcc tgtcgtggag
     2701 taaagcatgc gccgctcggc tgccgcgtcc gggtaggtca catcgacctg taagaggaaa
     2761 cggtcgagct gcgcttcggg caagggatag gtgccttcct gctcaagcgg gttctgagtt
     2821 gccagaacaa agaatggcga aggcagcgca taggtctggc ccgcat
//
LOCUS       DATMGX010000254         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_5936,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000254 DATMGX010000000
VERSION     DATMGX010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5936"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /gene="glnA"
                     /locus_tag="VNR65_04145"
     CDS             complement(<1..1024)
                     /gene="glnA"
                     /locus_tag="VNR65_04145"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HWJ17902.1"
                     /translation="MSNATEVVKKLKDEDIRFVDLRFTDPRGKLQHLTMETSEMGEEA
                     FAEGIMFDGSSIAGWKAINESDMTLMPDPESAHIDPFFAQSTLAIFCDILEPHSGERY
                     ERDPRGTARKAEAYLQQTGIGDKAYFGPEAEFFIFDDVRFSVDPYNCGYRLDSSELPT
                     NMPTEYEMGNLGHRPRTKGGYFPVPPVDSAQDIRSEMLSTMSAMGVVTEKHHHEVASA
                     QHELGIKFGSLTRMGDALQIYKYVIHNVAHSYGKTATFMPKPVFGDNGSGMHVHQSIW
                     KGGKPMMAGNKYADLSQDCLYYIGGIIKHAKALNGFTNPTTNSYKRLVPGYEAPVLLA
                     YSARNRS"
     gene            complement(1142..1480)
                     /locus_tag="VNR65_04150"
     CDS             complement(1142..1480)
                     /locus_tag="VNR65_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWJ17903.1"
                     /translation="MKKIEAVIKPFKLDEVKEALQEIGLQGITVTEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEIVLPEDMVEKAVEAIRNSARTGRIGDGKIFIINVEDAIRI
                     RTGETGPEAI"
     gene            1806..>2767
                     /locus_tag="VNR65_04155"
     CDS             1806..>2767
                     /locus_tag="VNR65_04155"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HWJ17904.1"
                     /translation="MLELLTAEEMSRAEQLAAAAGLSTLTMMENAGRSVAEEVVRRYP
                     RGSRVVVLCGPGNNGGDGFVCARFLRERGYHVRLALLGRQEDLKRDPKEMARRWDEAI
                     EPLGPQSLEGAQIIVDAIYGTGLRDEVSGVVAQMIEDVTGRGLPVISVDVPSGIDATS
                     GALKGLAFKAAATVTFFRRKTGHVLLPGRVYCGDVRAVDIGIPAAVLGEIAPRTFADD
                     PDFWLRYFPKLKTDGHKYDRGHALVVSGPMESTGAARLAARTSLRAGAGLVSVATSKS
                     AFYINAAQLTAVMVSPYDGPAGLSAILSDPRITAVLIGPGAGQEP"
BASE COUNT          519 a          786 c          845 g          617 t
ORIGIN      
        1 cggaccgatt gcgagccgaa taggcgagca ggacaggcgc ctcgtagccc ggaacgagcc
       61 gcttgtagct gtttgtcgtc ggattggtga agccgttcag cgccttggcg tgtttgatga
      121 tgccgccgat atagtagagg caatcctggc tgaggtcggc gtatttgttg ccggccatca
      181 ttggcttgcc gcccttccag atcgactggt ggacgtgcat gcccgagccg ttgtcgccga
      241 aaacgggctt cggcatgaac gtcgccgtct tgccgtagga atgcgcgacg ttgtggatca
      301 catacttgta gatctgcaac gcatcgccca tgcgggtcag cgagccgaac ttgatgccaa
      361 gctcgtgctg agcggaagcc acctcgtgat ggtgcttttc ggtgacgacg cccatcgcag
      421 acatcgtgga aagcatctcg gagcggatgt cctgcgcgct atcaaccggc ggaaccggga
      481 agtaaccgcc cttcgtacgc ggacggtggc cgaggttgcc catctcgtat tcggtcggca
      541 tgttcgtcgg aagttcagac gagtcgaggc ggtagccgca gttgtaagga tctacggaga
      601 agcgcacgtc gtcgaaaatg aagaattcgg cttcagggcc gaaatacgcc ttgtcgccta
      661 ttccggtctg ctgaagatag gcttcagcct tgcgggcagt gccgcgcgga tcgcgctcat
      721 agcgttcgcc cgagtgtggt tcgagaatgt cgcagaaaat cgcgagcgtc gattgcgcga
      781 agaacggatc aatatgcgcc gattccgggt cgggcatcag cgtcatgtcg ctttcgttga
      841 tcgccttcca gcctgcgatc gaggagccgt cgaacatgat gccctcggcg aaagcctcct
      901 cgcccatctc cgatgtttcc atggtcaggt gctgcagctt gccgcgcggg tccgtgaagc
      961 ggagatcgac aaaacggatg tcttcgtcct tgagtttttt tacaacttca gtcgcgtttg
     1021 acattgaact ccctcgtgca gttagggtcg cgtcgttggg gtgggttggc gattgggcgc
     1081 gccgattcga tcggctgctc ggtgggtatg gtattgcttc tgggagaagc ggggtagaac
     1141 gctaaattgc ctcaggtccg gtttcgccgg tgcggatgcg gatcgcatct tccacattga
     1201 tgatgaagat cttgccgtcg cctatccggc cggttcttgc cgagtttcgg atagcttcca
     1261 cagctttttc taccatgtcc tctggcagca caatctcaat cttaaccttg ggcagaaaat
     1321 cgacaacata ttccgccccg cggtaaagct cggtgtggcc cttctggcgg ccgaagcctt
     1381 tggcctccgt gacagtaatg ccttgaaggc cgatttcctg caaagcttcc ttaacctcat
     1441 cgagcttgaa cggtttgata accgcttcga tttttttcat gacgaatgaa ttcctccgaa
     1501 ggggacaaaa gcgcgctgca cccctctaaa gcacaggcca tgccaaacgc tgcatcgctt
     1561 atgacacaat gataaactcg gacttaacca aatcactctg cccaaaatag cgccatattg
     1621 catatctgtt gaccagaaga cgggcggtga gtggttagaa tgcggcgttt cgatatgtcc
     1681 ttcttgcttt cgcgaccctg cacaaaatcg gcgcttctct tccacgctcc tgcaaatttc
     1741 gctacattcg cccagatgtc cttttatgca tcgtcagaca gaaccgctga ctcacaggct
     1801 gcgccttgct tgaacttctg accgccgaag agatgagccg tgccgagcaa ctagccgcgg
     1861 ccgccggtct gtcgacattg acgatgatgg agaacgcagg ccgcagcgtt gccgaagagg
     1921 tcgtccggcg ctatccgcgc ggcagccgcg tggttgtctt gtgcggaccg gggaacaatg
     1981 gcggcgatgg gttcgtctgc gcacgctttt taagggagcg gggctatcac gtccgcctcg
     2041 cgcttctcgg acgccaggaa gacttgaagc gcgatcccaa ggagatggcg aggcggtggg
     2101 atgaggctat cgagccattg gggcctcaat cccttgaggg cgcgcagatc atcgtcgatg
     2161 caatctacgg cacgggcctg cgcgacgagg ttagcggtgt cgtcgcccag atgatcgagg
     2221 atgtgacggg ccgcggtttg cccgtcatct ccgtcgacgt tccttcgggc attgacgcaa
     2281 cgagcggcgc tttgaaaggt ctcgccttca aggcagcggc tacggtcaca ttcttccggc
     2341 gcaaaacggg gcatgtcctg cttcccggcc gcgtttattg cggggatgtc cgggcggtcg
     2401 atatcggcat tccggcggcg gtgctgggcg agattgcgcc ccggacattc gccgacgacc
     2461 cggatttctg gctgcgttat tttccgaaac tcaagaccga cggccacaaa tatgatcgtg
     2521 gccatgccct cgtcgtctcc ggtccgatgg agagcacggg tgcggccagg ctggcggcac
     2581 ggacatcgtt gagggcggga gccgggcttg tctcggtcgc aacgtccaag tcggctttct
     2641 atatcaacgc ggcgcaactg acggcggtga tggtctcgcc ctatgacggg ccggctggtc
     2701 tatcggcaat tctatccgac ccgcgcatca cagccgtcct gatcggcccg ggcgccggcc
     2761 aggaacc
//
LOCUS       DATMGX010000255         3549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_191039, whole genome shotgun sequence.
ACCESSION   DATMGX010000255 DATMGX010000000
VERSION     DATMGX010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191039"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..2644
                     /locus_tag="VNR65_04160"
     CDS             209..2644
                     /locus_tag="VNR65_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HWJ17905.1"
                     /translation="MDRYLCIHCHFYQPPRENPWLEAIELQDSAYPYHDWNERITAEC
                     YAANATSRILTGDNRITHIVNNYSRISFNFGPTLLSWLEEHAPATYQAILAADQESLQ
                     RFSGHGNAIAQVYNHMIMPLANRRDKQTQVVWGLGDFEHRFGRKPEGMWLAETAVDVE
                     TLEVLAEHDIKFTVLAPSQALHERRAGTTKFKNVEGGKIDPTRAYVCKLPSGRSINLF
                     FYDGPISRAVAFEGLLSDGETFANRLLSGFADNRRWPQLMHIATDGETYGHHHSRGDM
                     ALAYALHHIDSNKLAKLTNYGEYLEINPPTHEVEIVENTSWSCIHGVGRWSRDCGCNS
                     GGKPGWNQAWRAPLRDAFDHLREELRPEYERKASELLRDPWQARDEYINVVLDRSESS
                     LQAFFAKHGKAELTDADTVTALALLEMQRHAMLMYTSCGWFFDELSGIETVQTIMYAG
                     RALQLAQMLFPDHREERFKQLLALAKSNLAEDGDGARIFEKWVEPAEVNLLSVAAHFA
                     IASMFERIGESTSINCYEVHQRDDVRLESGRARLAIGTAHIRSRITHQQQEISFGVLH
                     FGDHNIIAGVRGFRGEQEFKAAARELRDCFQSADLPAVLRLLDKHFEGATYSLRSLFR
                     DERQKVVSKILGSTMDEAEAAYRQIYEHHAPLMGFLSDMGAPLPKMLHLTAEFVLNTA
                     LRKEFLADEIDLERIQTLLDTAAREKIQWDEAWLGYALNRRLVRMADELAVNPREERL
                     QRFNAAVSLVRSLPFEVNLGPVQNVFYQLLQNVYPSVALDGDESSQRWVSEFAGLGEK
                     LGVCVEPVAVETT"
     gene            3040..3318
                     /locus_tag="VNR65_04165"
     CDS             3040..3318
                     /locus_tag="VNR65_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWJ17906.1"
                     /translation="MNKGDLVEKIAGAHGISKTSAGGAIDTIIDAVTAALKKGGRVTL
                     VGFGTFSVSQRKARNGRNPQTGGVIKIAARKVAKFTPGVVLKKAVNKK"
BASE COUNT          753 a         1026 c         1083 g          687 t
ORIGIN      
        1 gatttcacgg atgaacacgg ataagtctta aatctaaaag cgttagatcc gtgctcatcc
       61 gtgtcaatcc gttggttaaa agcctttttt cggagttcgc gggagccaga cgcacttgcc
      121 tgaggttggc agccagcatc cgcgcgattc tagctcgcat cttggcatct aattttaggc
      181 gcaacatttt gacagcgcca cctcatacat ggaccgatat ctttgcattc attgccattt
      241 ttatcagccg ccgcgcgaga acccctggct tgaggcgatc gagctgcagg actcggcgta
      301 tccctaccac gactggaacg agcgcattac ggcggagtgc tacgccgcta acgccacctc
      361 gcgcatactg accggcgaca atcgcatcac ccacatcgtc aacaactatt cgcggatcag
      421 cttcaatttc ggccccacgt tgttgtcgtg gctggaggag cacgcgcccg ccacgtacca
      481 ggcgatcctg gccgccgacc aggaaagcct tcagcgtttt tccgggcacg gcaacgccat
      541 cgcccaggta tacaaccaca tgatcatgcc gctggcgaac cggcgggaca agcagacgca
      601 ggtggtctgg ggcctgggcg atttcgagca tcgcttcggc cgcaaacccg aaggcatgtg
      661 gctggcggaa accgcggtcg atgtggagac gctggaagtt ctagctgagc atgacatcaa
      721 gttcacagta ctggctccat cgcaagccct gcacgagcgg cgcgccggaa ccacgaagtt
      781 caagaatgtg gaaggcggca agatcgaccc aacgcgcgcc tatgtttgca agctgccgtc
      841 ggggcgcagc attaacctgt ttttttatga cggacccatc tcgcgcgcgg tggctttcga
      901 agggctgctg agcgacggcg agacttttgc caaccggctg ctcagcggat ttgccgacaa
      961 ccggcgttgg cctcaactca tgcacatcgc cacggatggc gagacctatg gccatcatca
     1021 ctccaggggc gacatggcgc tggcctacgc gctgcatcac atcgacagca ataagctggc
     1081 gaagctcacc aactacggcg agtatctgga gatcaatccg ccaacccacg aagtggagat
     1141 cgtggagaac acgtcgtgga gctgtatcca cggcgtgggc cgctggagcc gggactgcgg
     1201 ctgtaacagt ggcggcaagc cgggatggaa ccaggcctgg cgcgctccgc tgcgcgacgc
     1261 gttcgatcac ctgcgtgagg agctgcgccc ggagtatgaa cgcaaagctt ccgagttact
     1321 gcgcgatccg tggcaggcgc gcgatgagta catcaacgtg gtgctcgacc gcagcgagag
     1381 cagcctgcaa gccttcttcg ccaaacacgg caaggccgaa ttgaccgatg cagacaccgt
     1441 gaccgccctc gccctgctgg aaatgcagcg ccacgccatg ctgatgtaca ccagctgcgg
     1501 ctggtttttc gacgagttgt cgggtatcga aaccgtgcaa acgatcatgt acgctggccg
     1561 cgcgttgcag cttgcccaga tgctgtttcc cgaccaccgg gaagaaaggt tcaagcagct
     1621 gctggcgctg gccaagagca atcttgccga ggacggcgac ggcgcgcgca tcttcgagaa
     1681 gtgggtggaa ccggccgagg tgaacctgct gtccgtggcc gcccacttcg cgatcgcttc
     1741 catgttcgag cgaattggag agagcacctc tatcaactgt tacgaagtgc accagcgcga
     1801 cgacgtccgc ctggagtcgg gacgggcgcg gctggccata ggcaccgcgc acatccgctc
     1861 gcgcattacg caccagcagc aggagatcag ctttggggtg ctgcacttcg gcgatcacaa
     1921 catcattgcg ggagtgcgcg ggtttcgagg cgagcaggaa ttcaaggccg cggcgcggga
     1981 gctgcgtgac tgcttccagt cggccgacct acccgctgtt ttgcgcctgc tcgataagca
     2041 tttcgagggc gcgacctaca gtctgcgatc gctgttccgc gacgagcggc aaaaagttgt
     2101 cagcaagatc ctgggctcga ccatggatga agccgaggcg gcttaccgcc agatctacga
     2161 gcatcatgct ccgctaatgg gattcctcag cgacatggga gcgcctctgc cgaagatgct
     2221 gcacctgacg gcggagttcg tgctgaacac cgcgctgcgc aaggagttcc tcgccgatga
     2281 aatcgacctg gaacgcatcc agaccctgct ggatacggcc gcgcgggaga aaatccaatg
     2341 ggatgaagcg tggctgggct acgcgctcaa tcgccgtctg gtccgcatgg ccgacgaact
     2401 ggcggtgaat cctcgcgaag aacggctgca gcgcttcaat gccgccgtca gcctggtgcg
     2461 ctctttgccc tttgaggtaa acttgggacc ggtgcaaaac gtgttttacc agctcctgca
     2521 gaacgtgtac ccaagcgtcg cgctggacgg cgacgagtcc tcgcaacgct gggtcagtga
     2581 gtttgcaggg ttgggcgaga agctgggagt gtgcgtggaa ccggtggcag tcgagacgac
     2641 gtaggaactg gcaggtgtca gttgtcggtt gcccgaacta aagaggctgc tgaaaaagcg
     2701 acgttcgggt agtgcggacc ttcattcagc tccgcgtcca agccttttgt ttttgtcaga
     2761 tggagtacgg cttcagccga cggcaggctt gcttctcgga ctctttcagc agcttataaa
     2821 acgagcttta aggatgacaa aaaacaacaa gacttaccgg cgcgcctgaa ggtgcgcccc
     2881 ttcaaatcca ttgcaggact ccttcaactg agcgtactcc tcccttggtt tgcggcttga
     2941 tctgtggaaa accgcgaatt ttcacggttt ttcttgacat gggcccggtt tcgcaagtaa
     3001 atactggcgc cacaggttct gacctggcga ggagcgagca tgaacaaagg ggatctggtt
     3061 gagaaaatcg caggcgccca cggaatcagc aaaaccagcg ccggcggcgc gatcgacaca
     3121 ataattgatg cggtcacggc tgcgctgaaa aaaggcggcc gcgtgactct ggtgggattc
     3181 ggcaccttct ccgtctcgca gcgtaaggcg cgcaacggac gcaatccgca gacgggaggc
     3241 gtcattaaga ttgcggcccg gaaggttgcc aagttcactc cgggcgtcgt gctgaagaag
     3301 gccgtcaaca agaaatagct gaatcaattt cgttcgagcg gcccgccgcg gcgggcgact
     3361 tgcggcaggg cgaaagctct gccgcgttat tttttggtcg cgaggtcgag gatgaaagca
     3421 gcggatgcac gtaaagagga agtcaagctc taggcgcgcc tcgccgaatc gttttctccg
     3481 ggtccgaatc caatcgttta agacattggc ctccgctgga atgcgacgct ggcatgcgac
     3541 gattgccgc
//
LOCUS       DATMGX010000256         2278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_424845, whole genome shotgun sequence.
ACCESSION   DATMGX010000256 DATMGX010000000
VERSION     DATMGX010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424845"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VNR65_04170"
     CDS             <1..592
                     /locus_tag="VNR65_04170"
                     /inference="COORDINATES: protein motif:HMM:NF039288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWJ17907.1"
                     /translation="KLAEQLGSFAGQLTDNAPSLIRLEYAGEVAEMNTRALSSAAISG
                     VLRPFLHVNMVSGPAVAKERGIKVEEVRRGQEGAYESYMRVSVKTESQERAVAGTVFS
                     DGKPRIIQINKMPVDAEFAPNLLYTENDDKPGHIGALGTLLGSEGVNIATFNLGRDEP
                     GGKAIALVSVDEPVEETTLAKVRALPHVVRAARLSF"
     gene            complement(741..1832)
                     /locus_tag="VNR65_04175"
     CDS             complement(741..1832)
                     /locus_tag="VNR65_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17908.1"
                     /translation="MPKLHQSAVEKTPYTTLSCSPLEPLIVFNPVGVVEGPLVLNGRC
                     IAIAAFVCAAELAGCTTLPSLDPIDGTQAGPDGSISINEVVARVKCELYDATRDIRSD
                     YWWFRYWGAKVDLDLIVNEQAGLTPSVAFITPYAPFTSKVIGNISQSFSLGLTGGLTS
                     NAIRTETVSFSATFLEVRDQLDARVANNKLGRFNGCNLPEGSELNGNLGLKQWIDSAL
                     GPVKGGDDALLNWGGAVVASGGKSGKPKARALLPQESIASKACRWTPGSPPLTNPHCR
                     PPIDSITHTLEFTIVKSIGIAPTWTLVRFRGQTGNNSMASASRIDTHKLTISLGPSDE
                     NLSQASTNQSLRLLAPAITNAITQSQTPR"
     gene            complement(1959..>2278)
                     /locus_tag="VNR65_04180"
     CDS             complement(1959..>2278)
                     /locus_tag="VNR65_04180"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWJ17909.1"
                     /translation="SLAEGIHFAQKAGLDVGAVIETISKGAAQSWQMENRWMTMAEGK
                     FDFGFAVDWMRKDLAIVLDEARRNGAHLPLAALVDQFYSEVQAMGGRRWDTSSLIARL
                     DRK"
BASE COUNT          454 a          671 c          687 g          466 t
ORIGIN      
        1 gaagctcgcc gagcaactcg gctccttcgc tggccagctt accgataacg ctccaagcct
       61 gatccgcctc gaatatgccg gtgaggtcgc ggagatgaac acccgcgcct tgagctcggc
      121 cgcaatttcc ggggtgctgc gtccattcct gcacgtcaac atggtttccg gccctgccgt
      181 cgctaaggag cggggaatca aggtcgaaga ggttcgccgc ggccaggaag gggcttacga
      241 gagctacatg cgcgtttcgg tgaagaccga gagccaggag cgcgctgtcg cgggtaccgt
      301 attctccgac gggaagccgc gcatcatcca gatcaacaaa atgccggtcg atgcggagtt
      361 cgcgccgaac ctgctctaca cggaaaatga cgacaaaccc ggccatatcg gcgcgctcgg
      421 cacgctcctt ggcagtgaag gcgtgaacat cgccaccttc aaccttgggc gcgatgaacc
      481 gggcgggaag gccatcgcgc tggtcagcgt cgatgagccg gttgaggaga caacgcttgc
      541 gaaggtgcgt gcgctgccgc atgtcgttcg tgcagcccgg ctgagctttt aaatcaaaca
      601 ttcgacatcc cggaacttga ttacccggat gtctccgcca attccgtcgt catggccggg
      661 cttgacccgg ccatgacatc ggttccagct gaccggtcag cccggccatg acgtcggttc
      721 cagctaatcg caacacgttt ttagcgcggg gtctgcgatt gggtgatggc gttcgtgatg
      781 gctggcgcca agagcctcaa gctttgattt gtgctggctt ggctgagatt ttcgtctgat
      841 ggaccaagcg agattgtcag cttgtgggtg tcgatccggg aagcgctcgc catggaatta
      901 tttcccgtct gccccctgaa ccggacgaga gtccaggtcg gcgcaatgcc gatgcttttc
      961 acgatcgtga attcgagggt gtgcgtgatc gagtcgatgg gtggcctgca gtgcggattc
     1021 gtcaaaggcg gagagccggg tgtccagcgg caggccttcg acgcgatgga ctcctgcggc
     1081 agcaaagccc tggcctttgg ctttcctgac tttccgcctg aggcgacgac tgcgccgccc
     1141 cagttgagga gagcgtcgtc tcctcccttg acggggccga gcgcggaatc aatccactgt
     1201 ttcaggccca gattgccatt cagttcggag ccttccggga gattgcagcc gttgaatcgg
     1261 ccgagcttat tatttgcgac acgcgcgtcg agctgatctc tcacttcaag aaatgtggcc
     1321 gaaaaactga cggtctcggt tcggattgca ttactggtaa ggccgccggt caggcccaag
     1381 gaaaaggatt gcgagatatt tccaatgact ttggaagtga agggcgcgta aggcgtaatg
     1441 aaagcaaccg aaggcgtaag cccggcctgc tcgtttacga tcagatcgag gtcgaccttc
     1501 gcgccccaat atcggaacca ccaatagtcg ctgcggatgt ccctggtcgc gtcataaagc
     1561 tcgcatttta ctcttgcgac cacttcattg atggaaatgc tgccgtctgg cccggcttgc
     1621 gttccgtcga tcgggtcgag ggacggcaaa gtggtgcagc ccgccaactc agctgcacaa
     1681 acgaaagccg ctatggcaat gcaacgcccg ttcagcacca atggcccctc cacaaccccc
     1741 accgggttga agactatgag cggctccagc ggcgagcaag acagggttgt gtacggcgtt
     1801 ttttcaacgg ctgactggtg cagtttgggc acctggatct gtggatagcg gggaacggga
     1861 tgaacccgcc gttaatcagc gtcgattgaa ggcggaaaag ttgatcgatt ctcaagcggc
     1921 agcgcagctt atcctcgtcc aaatggacgt tggctcgtct acttgcggtc gagtcgcgcg
     1981 ataaggctcg acgtatccca gcgcctgccc cccatggcct gcacctcgga atagaactga
     2041 tcgacaagcg ccgccaaagg caaatgcgcg ccattgcgcc gggcctcatc caggacgatg
     2101 gcaaggtcct tccgcatcca gtcgaccgca aatccgaagt cgaacttgcc ctccgccatg
     2161 gtcatccagc ggttttccat ctgccaggac tgcgccgcgc ccttcgagat cgtctcgatc
     2221 acagcgccga cgtcgagccc ggccttttgg gcgaaatgga tgccctcggc aaggctct
//
LOCUS       DATMGX010000257         4123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_451718, whole genome shotgun sequence.
ACCESSION   DATMGX010000257 DATMGX010000000
VERSION     DATMGX010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451718"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(508..993)
                     /locus_tag="VNR65_04185"
     CDS             complement(508..993)
                     /locus_tag="VNR65_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17910.1"
                     /translation="MLGVALLRADCATHLTRRLDREPAHMSDVVARNEWRTKKVRDWL
                     LAILRFAVTLRDEDRSAVLVAGEEIDRLGKSSEDKSKFNFFRNTSIEICSAILEKQNL
                     RGSAVLRLHLNRIEDCRLRRAFAAAIKFGDSSQTIKSAGKFRKRVGEDLWKGLRPARD
                     G"
     gene            complement(965..1933)
                     /gene="dapA"
                     /locus_tag="VNR65_04190"
     CDS             complement(965..1933)
                     /gene="dapA"
                     /locus_tag="VNR65_04190"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWJ17911.1"
                     /translation="MPAIGQHVSKLSGYAPALPTPFSDDGNIDVAAFEQFCDRQVQEG
                     ATALVVCGTTGEAPTLSPAEHDTLIRIAVGVARDRIPVIAGAGSNSTAHAIELTRDAE
                     AAGADAILSVVPYYNKPIQRGLYAHFHAIAQSTGLPIIVYDVPSRTACGLADETVARL
                     AEIPQFIGLKDATGDVTRPPRLRLLVGPDFRLLTGDDATALAFLAQGGNGCISVTSNV
                     APGLCRNMFLACRQGQIASAQRWAIPVAKLTAALFRETSPAPLKYALSLLGLISPKVR
                     LPLVELTSQSKAEVAAVMAHICDHYAEHLIGKMCEPEHVGRRAVAG"
     gene            complement(1944..2699)
                     /locus_tag="VNR65_04195"
     CDS             complement(1944..2699)
                     /locus_tag="VNR65_04195"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ17912.1"
                     /translation="MADLRASGASSVSILDAGCGPGTWLRRLVTRARALGFTSITARG
                     FDIAHAQIQRARLLARNLSRLPGVNFTFDVADLTGRLPESDASVDITLCLYSVLSHLP
                     VASLTDISTDIARVTSGYFITTVRSIGSMPTAFVDSIEKVRQLKQDHVRNRCEIEFCD
                     GRRIAFSFHLFTASELRSYFASSFDIEDLRGLDLFHSRFIPDPRWNPACSRGDSQLSD
                     ELVRLEETYATSSEFMDRAAHLLLVARSAAKVA"
     gene            complement(2959..3366)
                     /locus_tag="VNR65_04200"
     CDS             complement(2959..3366)
                     /locus_tag="VNR65_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17913.1"
                     /translation="MDAMIQLARAAKSHRMIAAGPYSAEVFLELHRRGYPRVTTTKTS
                     RVPCGQYDVALVAWRAHSIKALETTLDWLVHFLSAAGVLVIWVGPHERMPNQTLNLAL
                     KRLGFRIESGTSCENGIAIAARRLDSVPAAKAA"
     gene            complement(3461..3751)
                     /locus_tag="VNR65_04205"
     CDS             complement(3461..3751)
                     /locus_tag="VNR65_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17914.1"
                     /translation="MKHAEPPSIQSCTRPSLFLIGKDSRGNWVVQEEQGNCGGLFVNR
                     AEALKFAKFENGNRPQAVIMVPGIFELDTSGKTALAHHLSHNTETPRERRVA"
     gene            complement(3785..3943)
                     /locus_tag="VNR65_04210"
     CDS             complement(3785..3943)
                     /locus_tag="VNR65_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17915.1"
                     /translation="MAWLRAARCSVAMAKRAYPVIFVFAIFATLLVATIALRLAIWLP
                     ISAIKSAF"
BASE COUNT          900 a         1125 c         1220 g          878 t
ORIGIN      
        1 gcgcacgcac ttggcattac agaaagatgc gcccttgcat cgagcagtcc aacgatctgg
       61 cgctattgtc gccattccca ttttggctgg gctgcatcat aaatacatcc ggatatgatt
      121 ttcggaaagg acagctatcc gtgcctttcg tgtgcgcatt ttcagtgggg cctggaggcc
      181 cgaaggcgag agtgggtcct accttggggc ggaaacgcgg ccgtgagcgt gcttctcgcg
      241 ccatttcggg ccggggccac gcatatagta cttttcgggc ttataggttc tcacatcaat
      301 ggcgatcagt atatgcaatg aatttaccaa ggacctccag gctcgtgcag ttgcggaacc
      361 cagaacagag ggcagggcgg agagtagctg gtccatatga attctccttg cccggtaatg
      421 tagggggggc ggcgcataag aattcgaggt ggggggatcg ttgttgttat aaatgggaga
      481 taagtggcaa ggtgcagact tggaacctta gccgtcgcgc gctggccgaa gtcctttcca
      541 gaggtcttca ccaacgcgtt ttctgaattt gccagcgctt tttattgtct gagaagaatc
      601 tccaaatttg attgctgccg caaatgcgcg cctaagacga cagtcctcaa ttctatttag
      661 atgaaggcgt aataccgcac ttcctcttaa attctgcttc tccaatatgg cgctgcagat
      721 ctcgatactc gtgttgcgaa agaaattgaa ttttgattta tcctctgagc tctttccaag
      781 cctgtcgatt tcttcgccgg ccaccagaac ggctgacctg tcttcatcgc gcagcgtcac
      841 ggcgaaacgc aaaatagcca gcaaccaatc tctgaccttc ttggtccgcc actcgttccg
      901 agcaactaca tcactcatat gggcgggctc cctatcgaga cgacgcgtga gatgcgtcgc
      961 gcaatcagcc cgcaacagcg cgacgcccaa catgttccgg ctcgcacatt ttgccaatca
     1021 gatgttctgc ataatggtcg catatatgtg ccatgacggc ggcgacctcg gccttgctct
     1081 ggctggtcaa ttcgaccagc ggcaatcgaa ccttcggcga tatgaggcca agcaggctca
     1141 acgcatattt gagcggagcc ggactggtct cgcggaatag cgcagcggtc aatttcgcga
     1201 ccggaatagc ccagcgctgc gcgctggcaa tctgtccctg tcggcaagcc agaaacatat
     1261 tccggcacag gcctggggcg acgttggacg tgaccgaaat gcagccgttg ccgccttgcg
     1321 caagaaaggc caaagcggtc gcgtcgtcgc ccgtaagaag cctgaagtct ggaccgacga
     1381 gtaatctcag ccgcggcggc cgagtaacat cgcccgtggc atccttgaga ccgatgaatt
     1441 gcggtatctc cgccaggcgg gcaactgttt cgtcggcaag gccgcatgct gttcgagacg
     1501 gcacgtcata gacgatgatt ggcaatccgg tcgattgagc gatggcgtgg aaatgggcgt
     1561 acaagccgcg ctgaatcggt ttgttgtagt acggcacgac ggacaagatg gcgtcggcgc
     1621 ccgcagcttc ggcgtccctg gtcaattcga tggcgtgggc ggtcgaattc gagccggctc
     1681 ccgcgatcac tggtatccga tcgcgagcaa cgccgactgc gatgcggatg agcgtgtcgt
     1741 gttctgcagg gcttaacgtg ggcgcttcgc cggttgttcc gcagacgacc agtgcggtgg
     1801 ccccctcctg gacttggcga tcacagaatt gttcgaatgc ggcaacgtcg atattcccat
     1861 cgtcgctgaa cggcgttggt agggcgggcg cgtagcctga cagcttcgaa acgtgttgac
     1921 cgatggcggg cataactgtt ccctcacgcg accttggcgg cactgcgcgc cacaagcaat
     1981 aggtgggcgg cacgatccat gaactccgag cttgtggcat acgtctcctc gagtcgcacg
     2041 agttcatcgg aaagctgact atcgcctcgc gaacatgcag ggttccaccg agggtcgggt
     2101 atgaatcgac tatgaaacag gtcgagacct cgcaaatctt cgatgtcgaa actgctcgcg
     2161 aaatagctcc gcagttcgga tgcagtaaat aggtgaaagc taaacgcaat acgtcggccg
     2221 tcgcagaact cgatttcgca acggtttcgg acgtgatctt gtttcaattg gcggaccttc
     2281 tcaattgaat ctacgaaggc cgttggcatg ctgccaatcg aacgcacggt agttataaaa
     2341 tatccggaag taacacgtgc gatatctgtt gagatatccg ttaggcttgc aacaggtaga
     2401 tgactgagga cgctgtaaag gcaaagagtt atatccaccg aggcatcaga ttcgggcaac
     2461 cggccagtaa ggtctgcgac atcaaatgtg aaattcactc caggaagcct ggaaagattc
     2521 cgggccagca accgagcgcg ttgaatttgc gcatgcgcaa tgtcaaagcc gcgcgcggtg
     2581 atgctggtga acccaagcgc gcgtgcgcgt gtaaccaacc ggcgcagcca tgtgccggga
     2641 ccacaacccg catccagaat gctgaccgag cttgccccgg aagcgcgcag gtcagccaac
     2701 tttttttcca gaagagtcca gacgcgccga tccgcatatg cgtgcattcc gtcaaaagaa
     2761 aacagttgcg tcggatcgcc gtcagcataa gcgacgtaat cgtcaccagc ctggttgtag
     2821 atagccgcga cgctctcggc cgttccctca tgaagctcca gggcagaatg cccaactctg
     2881 tgggagaacg tcggatcggc gggcgcgtag tctgtcagtt gcgaagcgtt tcgaccgatg
     2941 gcggacattg tcggagcctt atgcggcctt ggcggcggga accgaatcaa gccggcgcgc
     3001 ggcgatggca atgccgttct cgcagctggt gcccgattca atgcgaaagc ccaatctttt
     3061 cagcgccaga ttgagcgtct gattgggcat gcgttcgtgg gggccgaccc aaatgaccaa
     3121 aacgccagca gcacttagga agtgcacgag ccagtcgagg gtcgtttcga gagctttgat
     3181 cgagtgcgcc cgccaggcga caagagcgac gtcgtattgg ccgcacggaa cacgactagt
     3241 tttagtggtt gtcacacgtg ggtagccgcg gcgatgcaac tccaggaaga cctcagcgct
     3301 ataggggccc gccgcgatca ttcgatgtga ttttgccgcg cgtgcgagct ggatcatggc
     3361 gtccacgatc cgctcatccg agggattggg cctggattgg gtcatgtgta ccccccaaac
     3421 ggggacgacg gttggattgg atgggaacga ccgcaagcct tcaggccacc cgtcgctcgc
     3481 gtggagtttc ggtattgtgc gacagatgat gtgctagggc cgtcttgccg ctcgtatcga
     3541 gctcgaaaat accgggaacc ataataacgg cttgtggacg atttccattc tcaaactttg
     3601 cgaacttgag ggcttcggcg cgattgacga aaagtccgcc gcagttgccc tgttcttcct
     3661 ggaccaccca gttgccccgg ctgtccttgc cgatcaggaa gagcgaaggt ctcgtacacg
     3721 actgtatcga gggaggctca gcatgtttca tgactcgtct cctggatgat gtatgggggt
     3781 gctttcaaaa ggccgactta atggccgaga tcgggagcca gatcgcgagt cggagcgcga
     3841 tcgttgcgac aagaagggtg gcgaagattg cgaacacaaa aatgaccgga taggcgcgct
     3901 tcgccatcgc gacactgcat cgtgccgcgc gcaaccaggc cataggcaga tccgaggatc
     3961 tggatgacgg ctgacccacg atccgcgaaa cagcgccagg gcgagtggtg atcggcatat
     4021 caattctccg gatcatgggt cgccctgctt gcactcaacg cacaatcggc aaatcgtcgt
     4081 cgtttttcga cgaatgcttg taacggttgc ggtagagctc cgc
//
LOCUS       DATMGX010000258         2387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_133626, whole genome shotgun sequence.
ACCESSION   DATMGX010000258 DATMGX010000000
VERSION     DATMGX010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133626"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /gene="zorA"
                     /locus_tag="VNR65_04215"
     CDS             <1..1140
                     /gene="zorA"
                     /locus_tag="VNR65_04215"
                     /inference="COORDINATES: protein motif:HMM:NF033914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage ZorAB system protein ZorA"
                     /protein_id="HWJ17916.1"
                     /translation="DHNSHEGVQELVKQFSDTLQGGAGLELRELAGSLEAVLKAMETV
                     SSEMSRSGEDFAVKLGDAAQNLNRLVIDAGDNLSKQSEISRQTLEQMLASLRTIFDQA
                     NAQINTKLSSAAEGASSKLVEAMDRVLTKLEAQVGGLEQSFGGFREAATGQLAEVGRQ
                     MSEAQEKGAKSVADVSARAAAALEDGLSAAMKSIGREVYEFAAALRTSSTALGVQAEA
                     IDRASTRTRQTADVFGECAETVKAAMAPVVRSNVKTAEITAIVGEAVKQASAALEEGQ
                     LATRGLSQSIAAQVDRLTKLWENYERRFVKIDEDLERAFEKLAQETTKQSQLLAEQTI
                     RIDRGLAGAVDKLAGPVQGIGDGAQELSETVGELKKLFERARAPV"
     gene            1137..1997
                     /locus_tag="VNR65_04220"
     CDS             1137..1997
                     /locus_tag="VNR65_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWJ17917.1"
                     /translation="MIAEEESADYSDENVNYFVPMTDMMVGVLFIFILMLTAFALDFR
                     RTTDAQETALKVAQEVANKLDPLQSAVREQMALLDKAQQDRRELLQDIRTQLAGEGLS
                     VQIDEANGVLRLNEDAVRFAPNHADLVDRNKDNVGKIARVLERVLPKYVSCHNWQSSV
                     CSTAGDTALETLFIEGHTDTTGIDNSNWVLSTERAVNTYRELIAVSPALRLLRNNRNE
                     EVISVSGYSSTRPIDPRALRDAWDRNRRIDLRFVMDNDPRRNLEHILHVTDEMHQQIN
                     RLRAASEAAR"
     gene            1994..>2387
                     /locus_tag="VNR65_04225"
     CDS             1994..>2387
                     /locus_tag="VNR65_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17918.1"
                     /translation="MSLRQALNTFARSEPVTIALPKTTATSDVNERLAKIFGDVDTRQ
                     PLAENLDEFSRRMTYLLERDDPLDRRDLLRAPWCIWTSAHPLSRQPALVEGLLARIRE
                     AGRHNVYRMLAAAWLHNFKFGDFCVPLIG"
BASE COUNT          535 a          686 c          736 g          430 t
ORIGIN      
        1 gatcacaata gccatgaggg cgtgcaggaa ctcgtcaaac aattctccga cacgcttcag
       61 ggcggcgcgg gcttggaatt gcgtgaactt gcgggcagcc tcgaagccgt cttgaaagcg
      121 atggaaaccg tcagctcgga gatgagccgt tccggcgagg actttgctgt gaaactcggc
      181 gacgcggcgc aaaatctcaa tcggctggtc attgatgctg gagacaatct cagcaagcaa
      241 tctgagatca gccgccagac gcttgaacag atgctagcat ccttgcgcac gattttcgat
      301 caggcgaatg cgcagatcaa cacgaagctg tcgtccgctg cggagggggc atcttcgaag
      361 cttgtagaag cgatggatcg cgtcctgacg aagctcgaag cacaggttgg cggcctggaa
      421 cagagttttg gcggcttcag ggaagcggcc acgggacagc tcgccgaggt cgggcggcaa
      481 atgtcggagg cgcaggagaa aggcgccaag tcggtcgcgg atgtatcggc tcgcgcggct
      541 gccgctcttg aagacggtct ctctgcagcc atgaagtcga ttggtaggga ggtgtacgaa
      601 tttgcagcgg ctttgcggac gtcgtccacg gcgctcggtg tgcaagccga agccatcgac
      661 cgggcctcga cgcggacgag gcagaccgcc gacgtctttg gagagtgtgc ggaaactgtc
      721 aaagccgcca tggcccctgt agtgcgctcg aatgtgaaaa ccgcggagat tacggcgatc
      781 gttggcgagg ccgtcaagca ggcttcggcc gcgctcgaag aaggacagct ggcgacgcgc
      841 ggtctcagcc agtctattgc cgcgcaggtc gaccgcctca caaagctatg ggagaattac
      901 gagaggcgtt tcgtcaagat cgatgaggat ttggagcgcg ctttcgaaaa gctggctcag
      961 gaaaccacga aacaatcgca gctgctcgcc gagcaaacca ttcgaattga caggggactg
     1021 gcgggcgccg tcgataaact cgccggtccc gttcaaggga tcggggacgg cgcgcaggaa
     1081 ctctccgaaa ccgtgggcga acttaaaaaa ctcttcgaac gggcaagagc gccagtatga
     1141 tcgctgaaga agaaagcgcg gattactcgg acgagaacgt caattacttt gtccccatga
     1201 ccgacatgat ggtcggggtg ctcttcatct tcattttgat gctgacggca ttcgcgctcg
     1261 acttccggcg cacaaccgac gcgcaggaaa ccgcactgaa agtcgcgcag gaggtggcga
     1321 acaaactcga tccacttcag tcggccgtgc gcgaacaaat ggcgctgctc gacaaagcgc
     1381 agcaggatcg ccgagagctc ttgcaggata tacgaacgca gctcgcggga gagggactga
     1441 gtgttcagat cgacgaagcg aacggcgtcc ttcggctcaa cgaggatgca gtccggtttg
     1501 caccaaacca cgccgatctc gtggatcgca acaaggacaa tgtcggaaaa atcgcccgtg
     1561 tcttggaacg cgtcctgccg aaatacgtca gttgccacaa ctggcagtca tcagtctgtt
     1621 cgacggccgg agacaccgcg ctcgaaaccc tgtttatcga gggacacacg gatacgacgg
     1681 gcatcgataa tagcaattgg gtgctgtcga ccgagcgggc cgtcaacact taccgggagt
     1741 tgatcgccgt ctcacctgcc ctgcggctct tacgaaacaa tcggaacgag gaggtgattt
     1801 ccgtctccgg ctattcgtcg acgcggccca tcgatccgcg cgccctgcgc gacgcatggg
     1861 accgcaaccg ccgcatcgac ttgcgcttcg tgatggacaa tgatccccga aggaatcttg
     1921 agcacatcct ccacgttaca gacgaaatgc accagcagat caaccgcctg cgagccgcaa
     1981 gcgaggctgc acggtgagcc tccggcaggc gctgaacacg ttcgcccggt cggagcccgt
     2041 tacaatcgcg ctcccgaaga caacggcaac ctctgacgtg aacgaacggc tcgcgaagat
     2101 tttcggcgac gttgacacgc ggcaaccgct ggccgagaat ctcgatgaat tcagcaggcg
     2161 gatgacctat ctgctggaaa gagacgatcc gctcgaccgc cgcgatctcc tccgcgcgcc
     2221 ctggtgtatc tggacatcgg cacatccgct gagccgccag cccgccctcg tcgaaggatt
     2281 gctggctcgc atccgggaag ccgggcgcca caatgtttac cggatgcttg ccgcggcatg
     2341 gcttcataac ttcaagtttg gtgacttctg tgtgccgctc attggcg
//
LOCUS       DATMGX010000259         3943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_210741, whole genome shotgun sequence.
ACCESSION   DATMGX010000259 DATMGX010000000
VERSION     DATMGX010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210741"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VNR65_04230"
     CDS             complement(<1..1339)
                     /locus_tag="VNR65_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWJ17919.1"
                     /translation="MMPNAARLEGLLRAMSPDEKLGQLTMIRADFGEAGAELGAAKIT
                     EIQAGRTGSILDLRGLEHIQKSQRAAVEGSRLGIPLLFTLDVLHGYETIFPIPLGEAA
                     AFDPELWQQTASAAAAEAAAEGIALTYAPMLDVGRDPRWGRIAESPGEDPFVGARFAE
                     AKVRGFQHGDLSQGAALAATAKHFAGYGAVTAGRDYASADIAPRTLEEVHFPPFAAAV
                     HAGVAAIMPAFIDLAGEPLTAHRGMLDGILRRRWGFDGVIVSDFCAVRELIVHGVAAD
                     AVEAAALALRAGVDIDMESGVYLEGLPEALSRGLVSQDEIDVAVRRVLMLKARLGLFD
                     KPFERGSRPLSSPQRQQHRALAREAARKSIVLLQNLNAVLPLRGEGGHLAITGPLSDA
                     ARDMLGPWHAAGNVKETVSFLDGVKKALPGWRITDGASLDAAREADAVLLCIGE"
     gene            1426..3246
                     /locus_tag="VNR65_04235"
     CDS             1426..3246
                     /locus_tag="VNR65_04235"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HWJ17920.1"
                     /translation="MPPNKLAPGIADESLLGQMRFFWKMLDGPKSKHSVTKFFVLIAF
                     VIGANALTQVQLNNWQGTIYDAIGQRDLSVFLHEIGVFLTIVSILLCLGVLQTWLHER
                     MKVKIRQAATLDLLDEWLQPARAFLLPLTGEISINPDQRIQEDTRRLSELSVDLAVGL
                     VQSTLMLLAFVGVLWQLSAQVVFIIQGKSVSIPGYMVWAAIAYALVGSFITLLVGRPL
                     ISAHTQLRAAEAAFRFDLVQLNESAENIALCRGEAVERETLNGPVDAVLTIMRRIANR
                     LATLTWVTGGYGWLAILAPLLLAAPGYFGGTLSLGGLMMVVGAFYQVQTALRWYVDRF
                     PALAEWRAMLTRVIDYGSALERVQYLDGVAGHIRYGSSSSDELIMENLCVLAPNGHVS
                     LGERFVAIAPGERVLIVAPPKSGKTTFIKALARLWVWGSGTIRFPEGTRMMFVPQSPY
                     HPAGALKAALTYPEPADRYTDAEAIRVLERVNLGRLGPQLGLKKRWNKELTLEERWRL
                     VLSRVLLHSPDWVIYDESIAELDDESRKIALSIFSSELAKTAVVSVGRLAPGHGFYQR
                     TLKLQTRLPGLKLPLHFVNGAPLKEKAATTTTEKVATCSC"
     gene            3320..>3943
                     /locus_tag="VNR65_04240"
     CDS             3320..>3943
                     /locus_tag="VNR65_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17921.1"
                     /translation="MNGILGPSLLKPERMTDDALLELVQRQTFRYFWDFAHPKSGMAR
                     DRSGPDGGGSNDLLAVGGTGFGIMAMIVAVERGWISRAEAIGRLLDMLDYLKAAEQYN
                     GVFPHYLDGPTGKEVSFWADNAGGDIVETSYLVQGFLCARQYFCESDPKEKALCAGVD
                     DLWRKVNWNWHTKGADVLYWHRNAKTEKENHHRIEGWNECLITYVLAA"
BASE COUNT          763 a         1198 c         1178 g          804 t
ORIGIN      
        1 tttcgccgat acagagcagc acggcgtcgg cttcgcgagc cgcgtcgagc gatgcgccat
       61 ctgtaatccg ccagccggga agcgctttct tgacgccatc gagaaagctc acggtctctt
      121 ttacattccc cgccgcatgc cacggcccca gcatatcgcg ggctgcgtcc gagagcggcc
      181 cggtgatggc gagatgaccg ccctcgcccc ttagtggcaa aacggcgttc aggttttgca
      241 gaagcacgat ggatttgcgc gcggcctcac gggcgagcgc tcggtgctgc tggcgttgcg
      301 ggctcgacag aggacggctt ccacgctcga aaggcttgtc aaaaagccca aggcgcgcct
      361 tgagcatgag cacgcgccgg acggccacat cgatctcatc ttgcgaaaca aggccgcggg
      421 acagcgcctc gggcagccct tcgagataga cgccgctctc catgtcaata tcgacgcccg
      481 cgcgcagggc gagcgccgcc gcttcaacag catcggccgc cacgccatgc acgatcaatt
      541 cgcggacggc gcagaagtcg ctgacgatga ccccgtcaaa cccccaacgc cgccgcaaaa
      601 tgccgtcgag catcccgcga tgggcggtca gcggctcgcc ggcgagatcg ataaaggctg
      661 gcatgatcgc cgccaccccc gcatgcacgg cggcggcgaa gggcgggaaa tgcacctctt
      721 cgagcgtgcg cggcgcgatg tcggcggacg catagtcccg gcccgcggtt accgcgccat
      781 agcccgcgaa atgcttcgcc gtcgcggcga gcgccgcccc ctgggataga tcgccgtgct
      841 gaaaccctcg cactttcgcc tcggcgaagc gagcgccgac aaatggatct tcgccggggc
      901 tttcagcgat tcggccccag cgggggtcgc gtccgacgtc aagcatcggc gcgtaggtca
      961 gcgcaatccc ttctgcggcc gcctcggccg ccgccgcgct tgcggtttgt tgccacagct
     1021 cggggtcgaa ggcagccgct tcgccgagcg ggattggaaa gatagtctcg tacccatgca
     1081 gcacgtcgag ggtgaagagc agcggaatgc cgaggcgtga cccctccact gctgcccttt
     1141 gcgatttttg aatgtgttcc aagccccgaa ggtcgaggat gctccctgta cgtcccgcct
     1201 gaatctccgt gattttggcc gcgcccagtt ccgcccctgc ctcgccaaag tctgcgcgga
     1261 tcatggtgag ctggcccaac ttctcgtcgg gggacatcgc ccggaggaga ccttcgagtc
     1321 gtgcagcatt aggcatcatg gcaacccctg gtggtcaatt gctaccttta ggcttataaa
     1381 ggtggtaaac ggtcgtcaac catccaccgc ttaggaaagg aatccatgcc gccaaacaag
     1441 ctcgcaccag gaatcgcaga cgagagcctt ctcggtcaaa tgcgattttt ctggaagatg
     1501 ctcgatggcc ccaaatcaaa gcacagcgtt accaagtttt ttgttcttat cgcgtttgtt
     1561 ataggggcaa atgcacttac acaggtacag ctcaataatt ggcagggcac catctatgac
     1621 gccatcggcc agcgtgatct ttccgtgttt ctgcacgaga tcggtgtatt ccttacaatt
     1681 gtaagcattc tcctttgcct gggggttctg caaacgtggc tgcatgagcg gatgaaggtc
     1741 aagattcgcc aagccgccac tttagacctt ctcgacgagt ggctgcagcc cgcgcgtgcc
     1801 ttcctcttgc cgcttacggg cgaaatcagc atcaatccgg accagcgcat ccaggaagat
     1861 acgcggcgcc tttcggaact gtccgtagac cttgccgtcg gcctcgttca atcgacgctc
     1921 atgctcctcg ctttcgtcgg cgtgctctgg caattgtcgg cgcaggtcgt tttcatcatt
     1981 caaggcaagt cggtcagcat ccccggttac atggtctggg cagcgatcgc ctacgccctt
     2041 gtcggctcgt tcataacctt gctcgtcggc aggccattga tcagcgcgca cacgcagctg
     2101 cgcgcggccg aagcagcctt ccgcttcgat ctcgtccaac ttaacgagtc cgccgaaaac
     2161 atcgcgcttt gccgcggcga ggcagttgaa agggaaactc taaacggccc cgtcgacgcc
     2221 gtcctgacaa tcatgcggcg catcgcgaac cggctggcga cgctcacgtg ggtcaccgga
     2281 ggctatggct ggctcgcgat ccttgctccg cttcttcttg ctgcaccggg ctatttcggc
     2341 ggcactcttt cccttggcgg cttgatgatg gttgtcggtg ccttttacca ggtgcagacg
     2401 gcccttcgct ggtacgtaga tcgtttccca gcccttgcag aatggcgcgc catgctcacg
     2461 cgcgtgatcg attacggcag cgcgctggaa cgcgtgcaat acctggatgg cgtggccgga
     2521 catatccggt atgggtcaag ctcgtctgac gagctgatca tggagaacct ttgcgtgctc
     2581 gcgccgaatg gccacgttag ccttggagaa cggtttgtag cgatcgcccc gggtgagcgc
     2641 gtccttatcg ttgcgccgcc gaaatcggga aaaaccactt ttatcaaagc gcttgcccgt
     2701 ctctgggttt ggggaagcgg caccatccgt ttccccgagg gcacgcgcat gatgtttgtg
     2761 ccgcagtcac cgtatcatcc agccggggca ttgaaggcag cgctcaccta tcccgaacca
     2821 gcagaccgtt acacagacgc cgaggctata agagtgcttg agcgggtcaa tttggggcgg
     2881 cttggcccgc aactcggctt gaaaaagcgt tggaataaag agcttacgct ggaagagcga
     2941 tggcgcctcg tccttagccg tgtgctcctt cacagcccgg attgggtcat ttacgacgag
     3001 tccatcgccg aactcgatga tgaaagcagg aaaatcgctt tgtcgatttt ctcgtcggag
     3061 ctggcgaaaa cagcggttgt cagcgtcggc cggcttgcac cggggcatgg attctaccag
     3121 cgcaccttaa agcttcagac gcggcttccg gggctgaagc tcccgctcca ctttgtgaat
     3181 ggcgcccctc taaaagaaaa ggcggcgacg acgacgaccg aaaaggttgc gacctgcagt
     3241 tgctgattgg tcacgagttt ttctttttcg tgtgtgagat ggagcacaac tttgcggcga
     3301 gttcactcga gggacggcga tgaatggaat tttggggccc agcctcttga agcctgaacg
     3361 catgacggac gatgcgctct tggaattagt tcaacggcaa accttcaggt atttctggga
     3421 ctttgcgcac cccaagagcg gcatggcccg tgatcgctct ggcccggacg gaggcggaag
     3481 caacgacctc ctcgcggtcg gcgggaccgg cttcggcatc atggccatga tcgtggccgt
     3541 cgagcgggga tggatttcgc gcgcggaggc tatcgggcgc ctgctcgaca tgctcgatta
     3601 tctgaaggcc gccgagcagt ataatggcgt ctttccccat tatctcgacg gtccgaccgg
     3661 gaaagaagtc tccttctggg ccgacaatgc gggcggcgac atcgtcgaaa cgtcctatct
     3721 cgttcagggt tttttgtgcg cacgccaata tttttgcgag agcgatccga aggagaaggc
     3781 gctctgcgcc ggcgtcgacg acctctggag aaaggtgaac tggaactggc acacgaaagg
     3841 cgccgacgtc ctgtactggc atcgcaacgc aaaaaccgag aaggaaaatc accataggat
     3901 cgagggctgg aacgaatgcc tgatcaccta tgttctggct gcc
//
LOCUS       DATMGX010000260         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_410764, whole genome shotgun sequence.
ACCESSION   DATMGX010000260 DATMGX010000000
VERSION     DATMGX010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410764"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="VNR65_04245"
     CDS             <1..1690
                     /locus_tag="VNR65_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HWJ17922.1"
                     /translation="NFLVITKGATRIAEVGARFTLDAIPGKQMAIDADLAAGSIDEKQ
                     ATHRRRELEEESAFFGAMDGASKFVRGDAIAGMIITAINVFGGIIIGVLRHDMSLSSA
                     ADVFVRLSVGDGLVTQIPALIVSLAAGLLVSKGGTRGSADKAVLDQLGNYPRAVFVAG
                     LVSAVFALTPGLPFFPFALLAGTAMFSAYAVPRWKAEVIAKDEAQAARVRAEADEETR
                     QSVKESLKLAGIELCLGKQIASVLLPSHTELGQRVANMRRKFAKQYGFIVPEIKLSDD
                     IFMQPKAYQIKIHGALVAGSELRIGEILVVTGDGPVPRVPGDETREPAFGMKALWVPE
                     VFSATLKSEGFTPVDNMSVLLTHLGETIRNNLAQLFSFKDYRKLMDGLDPEYKRLLDE
                     VVPSNISNSGLHAVLRRLLSERISIRNLNLILEAVAEIAPHVGRTEQIAEHVRARMAQ
                     QICADFSQDGMLKILRLGQRWEVTLHQALRRDAKGEVVEFDIDPALIEQFSAELSKAV
                     RDLMDRGTVFVLVAAPDIRPYVRMVTERMFPNLPVLSHLEIARGVPISQVGSVS"
     gene            1687..>2367
                     /locus_tag="VNR65_04250"
     CDS             1687..>2367
                     /locus_tag="VNR65_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HWJ17923.1"
                     /translation="MIAAALAALPSFVLSVFLVFCRVGACLMLIPAIGSARVPVQVRL
                     MLSLGASAAVAATIGEFAELRQGIPPLATVASLVAVETGKGLFIGFMARFFFAMLQFL
                     AEAAANAIGLSPMISSAEDGESMPAMAALITITASALFVITDQHLEVLRALVQSYDVM
                     AFGASLDQRVEMINILGALANASLLSLQVCSPFLVFAVTINFVFGLLNKLAPQIPVTF
                     VSAPFVVGG"
BASE COUNT          448 a          676 c          717 g          526 t
ORIGIN      
        1 caattttctc gtcattacca aaggtgcgac acgaatagcc gaggtagggg caaggttcac
       61 gctggacgcc atcccgggca aacagatggc catagacgcg gatctcgctg ccggatcgat
      121 cgacgagaaa caggccacac accggcgccg cgagttggaa gaggaaagcg cattcttcgg
      181 ggcaatggat ggcgcgtcga aatttgtgcg cggcgacgcg attgccggca tgatcatcac
      241 tgcgatcaat gttttcggcg ggataatcat tggggtgctg aggcacgaca tgtcgctgtc
      301 gtcggcggca gatgtgttcg tgagactgtc ggtcggcgat ggcctggtaa cgcagattcc
      361 cgcgctgatc gtctcgcttg ccgctggttt gttggtttca aaaggcggaa cgcgcgggtc
      421 tgctgataaa gccgttctgg accagctcgg caactaccct cgcgcggtgt tcgttgccgg
      481 gctcgtttct gcggtgttcg cattaactcc ggggttgccg ttttttccct tcgcgctctt
      541 ggcaggaacg gcgatgttct ccgcctacgc agtgccgcgc tggaaggcgg aggttatagc
      601 caaagacgaa gcccaggccg cgcgtgttcg cgctgaggcc gacgaggaaa cccggcagtc
      661 ggtcaaggag tccctgaaac tcgcgggaat agagctttgc ctcggcaaac aaatcgcaag
      721 cgtccttctg cccagtcaca cggaacttgg gcagcgcgtc gcgaacatgc gccggaagtt
      781 cgcaaagcag tatggcttca tcgtcccgga gatcaaactc tccgacgaca tcttcatgca
      841 gcccaaggct taccagatca agattcacgg ggcgcttgta gcaggcagcg aattgcggat
      901 cggtgagatc cttgttgtta ccggcgatgg gcctgtgccg cgcgttcctg gcgacgaaac
      961 ccgggaacct gcttttggca tgaaggccct atgggtgccg gaagtcttct ccgcgacgct
     1021 caagagcgag ggatttacgc ccgtcgacaa catgtcggtg cttctgacgc accttggaga
     1081 aacgatccgc aacaatctcg ctcagctgtt ttccttcaaa gactaccgca agctgatgga
     1141 cgggctcgat ccagaataca agcgcctcct ggatgaggtc gtcccgtcca acatctccaa
     1201 ttccggcctg cacgccgttt tgcgcaggct gctttcggag cggatctcga tccgcaatct
     1261 caatctgatc ctggaagccg tggcggaaat agcgcctcac gtcggtcgaa ccgagcagat
     1321 tgccgagcat gtgcgagcaa gaatggcgca gcagatttgc gccgatttct ctcaggacgg
     1381 catgttgaaa attcttcggc tcgggcagcg ctgggaagta acgctccacc aggcgctgcg
     1441 gcgggatgcg aagggcgagg tggtcgagtt cgacatcgat ccggccttga tcgagcaatt
     1501 cagtgccgag ctgtccaagg cggtgcgcga ccttatggac cgggggacag ttttcgtcct
     1561 tgtggccgcg ccggatatcc ggccctacgt ccggatggtg acagaacgga tgttcccgaa
     1621 tttgccagtg ctgtcccacc tggaaattgc ccgcggggtg ccgataagcc aggtcggttc
     1681 cgtctcatga ttgcagctgc gctcgcggcg ctcccaagtt tcgttttatc ggtgttcctg
     1741 gttttctgcc gggtcggcgc ctgcctcatg ctcataccgg caatcggcag tgcaagagtt
     1801 ccggtgcagg tgcgccttat gctcagtctt ggcgccagcg cggcagtggc agctaccata
     1861 ggggaatttg cagaattgcg ccaaggtatc cctccgcttg caactgtggc aagccttgtc
     1921 gccgtcgaga ctgggaaggg attattcatc ggcttcatgg ctcgcttctt tttcgcgatg
     1981 ctgcaattcc tggccgaagc ggcagctaac gccatcggcc ttagtcccat gataagctcc
     2041 gcggaggacg gcgagtcgat gccagcgatg gcagcgctta tcacgattac agcgtctgcc
     2101 ctcttcgtca taaccgacca gcacctggaa gttctgcggg cattggttca atcttacgac
     2161 gtgatggcct tcggagcgag cctggatcag agggtcgaga tgatcaacat tcttggcgct
     2221 cttgccaacg cgagcctgct gtcgttgcag gtgtgttcac ccttcctcgt ttttgccgta
     2281 acgatcaact tcgtgttcgg gctcctcaac aagctggctc cccaaatccc cgtaaccttt
     2341 gtttcggcgc cattcgttgt gggcggc
//
LOCUS       DATMGX010000261         2714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_423767, whole genome shotgun sequence.
ACCESSION   DATMGX010000261 DATMGX010000000
VERSION     DATMGX010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423767"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VNR65_04255"
     CDS             complement(<1..277)
                     /locus_tag="VNR65_04255"
                     /inference="COORDINATES: protein motif:HMM:NF036989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17924.1"
                     /translation="MSENVRELRPKTGDTEKITINLGFVDLGHVDLLVQEGFYSNRTD
                     LIRTAIRNQIERHADVVRKAVTRKSVDLGLRHFTRADLEAARDAGHML"
     gene            309..659
                     /locus_tag="VNR65_04260"
     CDS             309..659
                     /locus_tag="VNR65_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17925.1"
                     /translation="MIDSQLQGLALAIVEENSLSDRSSSESAQISKIHNFGGTMTAEI
                     GSLVKWGKSSPTRLGYKASDIGKVIGVHNGRSGGDELDVEFPDGDVIRGAIEGWFERA
                     QLGSEEEAPDTRPS"
     gene            940..2319
                     /locus_tag="VNR65_04265"
     CDS             940..2319
                     /locus_tag="VNR65_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HWJ17926.1"
                     /translation="MTMTSFGKRTTPAEPAAGGLPSGRAPVTGAPQMAPSALKTADFI
                     RDQVLLRIEPVAAVRMNKQELTAFVNSLVAEIANERKLLLNQTEQDALTRDIVDEMIG
                     LGPIEPLLHDPTVSDILVNGPHMIYVERRGKLELTGLRFRHDAHVLHVAQRIASFVGR
                     SVDESNPMLDARLADGSRVNVIIPPLSLKGPSISIRKFSQKILSLKHLAAAGSMSPGL
                     ATALEIFARCRLNIIVSGGTGSGKTTLLNALSGMIDPGERIVTIEDAAELQLQQKHVI
                     QLETRNANLEGRGKIDQGGLLRNALRMRPDRIIVGEVRGGEAFDMMQAMNTGHDGSMS
                     TVHSNSARDALARLENMVLMGNPNIPVRSIRGQISSAVDVVIHTERMRDGARRVTEVA
                     EVMGLEGEIVVIGTLFNFEYKGEKPGGGLDGEFKSTPVAPRFLPRIKYYGLDEQLLDA
                     MGVQKRWSS"
     gene            2415..>2714
                     /locus_tag="VNR65_04270"
     CDS             2415..>2714
                     /locus_tag="VNR65_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17927.1"
                     /translation="MEQRLRLLSSASESVEPEIESGTLAKPKTKVLQLERLLLLDRDH
                     PWNLEVDPAKLVIGAIAAAGLTWLAFHNMLQAPIALSAAAACLGAYLAMRIVVIRE"
BASE COUNT          570 a          756 c          810 g          578 t
ORIGIN      
        1 cgagcatgtg gccggcgtcc cgcgcagctt cgaggtcggc ccgcgtgaaa tgccggaggc
       61 cgagatcgac gctcttacgt gtcacggcct tccggaccac gtcggcgtgg cgctcgatct
      121 gattgcggat ggctgttcga attaagtcgg tgcgattgga gtagaagcct tcctgcacga
      181 gcagatcgac atggccgaga tcgacaaagc ccagattgat cgtgattttt tcggtgtcgc
      241 ccgtcttggg gcgcagctct cgcacattct ctgacatcgc cttcaccatc catctggatg
      301 gtatatggat gatagattcc caacttcaag gtctcgcgct tgcaattgtg gaagaaaaca
      361 gcctctcaga ccgttcttcc tccgagagtg cgcagatatc caagattcat aactttggag
      421 ggactatgac agcggagatc ggatccttag taaaatgggg aaagtccagc ccgacgcggc
      481 ttggctataa ggcctccgac atcggaaaag tgataggcgt tcataatggc cggtccggcg
      541 gtgacgaact tgatgttgaa tttcctgacg gcgacgtgat ccgtggagct atcgaggggt
      601 ggtttgaacg tgcgcaactc ggaagtgaag aggaagcgcc cgatacaaga ccatcgtaga
      661 acacctgcga acgaggaacg gagccacaaa acccagaaaa ctggcgcaag agcatcgaga
      721 gggcggcacc ggcaaccaat tgaattgccg gctttgcaca ccatcgtcac cgcgatgctc
      781 aggctgttac ccggctcaga gaccgcaatc cccacgggca gaagcttatc attgcatctc
      841 tggctgttgg ctgaagacgg cttgtttcaa tcttaaattg tgtcgatgtc ataaattaac
      901 cgccgcccgc ctaaagtgga tgcgttcacg atcgggcgca tgacgatgac ttctttcggc
      961 aagagaacga caccggcaga accggctgcg ggcggcttac caagcggccg tgcccccgtt
     1021 actggcgcac cacagatggc gccttccgct cttaagactg cggactttat tcgcgaccaa
     1081 gtcttgcttc ggatcgagcc ggtcgcggca gtgcgcatga acaagcagga attgacggcg
     1141 ttcgtaaata gcctcgtggc tgagatcgcg aatgagcgga agctgcttct gaaccagacc
     1201 gagcaggacg cgctgacccg tgacattgtc gatgaaatga tcgggttggg cccgatcgag
     1261 ccgctgctgc atgatccaac ggtgtctgac atcctcgtga acggccccca tatgatctat
     1321 gtggagaggc gcggcaagct tgaactcacc gggctgaggt ttcgccacga tgcgcacgtc
     1381 ctgcacgtcg cgcagcgtat cgcgtccttc gtcggacgca gtgtcgacga atccaatccg
     1441 atgctggatg cgcgcctcgc cgacggcagc cgggttaacg ttatcattcc tccgctgagc
     1501 ctgaaggggc cttccatatc aatccggaag ttctcgcaaa agatcctgag cctcaagcat
     1561 ctggccgcgg cgggctcgat gtcgccgggg ctcgccaccg ctttggagat tttcgcacgc
     1621 tgccgcttga acatcatcgt ttccggaggc acgggctccg gcaagaccac gttgctcaac
     1681 gccctctctg gaatgatcga cccgggcgag cgaattgtca cgattgagga cgccgccgag
     1741 ttgcagctcc agcagaagca cgtcattcag cttgaaacac gcaacgcaaa tcttgagggg
     1801 cgcggcaaga tcgaccaggg gggcctgctc cgcaacgcgc tacgcatgag gccggaccgt
     1861 atcattgtcg gcgaggtccg cggaggcgag gctttcgata tgatgcaggc catgaacacg
     1921 ggccacgatg gctcgatgtc gacggtgcat tccaactcgg cgcgggatgc tttggcgcgt
     1981 cttgagaaca tggttcttat gggcaacccc aatatccctg tccgctccat acggggacag
     2041 atctccagcg cagtcgatgt cgtcattcac acagaacgca tgcgcgacgg cgcccggcgc
     2101 gtcaccgaag ttgcggaggt gatggggctc gagggcgaga ttgtcgtgat cggtacgctt
     2161 ttcaatttcg aatataaggg agaaaagccc ggtgggggcc tcgacggcga gttcaagtcc
     2221 acacccgttg ccccgcgttt tcttccacgt atcaaatatt atggcctgga tgagcaattg
     2281 ctggatgcca tgggcgtcca gaaaagatgg agtagttaag aatgctgctc ccgattttat
     2341 tattcgcttt ggtgctgtgc gcaggactta catttggttt ggcgttgacg gtctcgcgaa
     2401 gaaggcggcg cgaaatggaa cagcgtctgc gccttctttc ctcggcatcc gaaagcgtgg
     2461 aaccggaaat cgagtctggc accctggcaa agccgaagac caaggtcctg caacttgagc
     2521 ggctgctcct tttggatcgc gatcatcctt ggaatttgga ggttgatccg gcgaagctgg
     2581 tgattggagc tatcgccgcc gccgggctga cctggcttgc ctttcacaac atgctccagg
     2641 ctccgattgc cttgtctgcg gcggctgctt gccttggcgc ttacctcgcg atgcgaattg
     2701 tcgtcattcg cgag
//
LOCUS       DATMGX010000262         4725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_111302, whole genome shotgun sequence.
ACCESSION   DATMGX010000262 DATMGX010000000
VERSION     DATMGX010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111302"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="VNR65_04275"
     CDS             <1..1825
                     /locus_tag="VNR65_04275"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HWJ17928.1"
                     /translation="DKSRSEVLPLLLHGDAAFAGQGVVAECFGLSGLKGHRTGGSLHF
                     IVNNQIGFTTAPRFSRSSPYPSDIARMIEAPIFHVNGDDPEAVVYCARVATEFRQRFH
                     KTVVIDMICYRRHGHNEGDEPSFTQPLMYKRIREQPSVVSIYSKRLAEEGVVKEAEAE
                     KIRTGFWDMLETEFEASEGFRPNKADWLDGRWSGITLAEEGPRRGKTGVSIDTLKQIG
                     AKLTEVPKGFTLHKTIDRLLANRRKMIGEGRGIDWAMAEALAFGTLLEEDFPVRLSGQ
                     DVERGTFSQRHSVLTDQLNEEQYIPLNHISNRQARFEVINSMLSEEAVLGFEYGYSLA
                     EPNTLVVWEAQFGDFANGAQVVIDQFLASGERKWLRMSGLILLLPHGYEGQGPEHSSA
                     RLERYLQLSAEDNWQVANCTTPANYFHILRRQLHRGFRKPLILMSPKSLLRHKRVVSP
                     IEDFRPETTFHRVLWDHAQLGGSEKVKLAPDMDIQRVVLCSGKVYYDLFEERETRGNG
                     EIYLMRLEQLYPFPARALIQELSRFPNAEIVWCQEEPKNQGAWTFVEANIEWVLDHIG
                     ADSRRPRYVGRPACASTAAGLMSKHTAELHAFLDEALTLQG"
     gene            1884..3236
                     /gene="odhB"
                     /locus_tag="VNR65_04280"
     CDS             1884..3236
                     /gene="odhB"
                     /locus_tag="VNR65_04280"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HWJ17929.1"
                     /translation="MTRSLRSLLSMLAKDLRMAIEIVVPTLGESVTEATVGKWFKKAG
                     DAVKADEALAELETDKVTLEVNAPAAGVISEIVIKEGETVAVGAILGSIAEGQAAAAA
                     PAVKAAEKPIQVKPAGAPQSVQASPAPEQRASSAQQAAASRSPNAETLAPSVRRIAEE
                     SGIDPAAVTGSGKEGRLTKGDMLAVIEGGASTGGAISFGKLQAPPPSAPRAPYAPPRE
                     ALEERVRMTKLRQTIARRLKEAQNTAAMLTTFNEVDMTTIMEVRNRYKDLFEKKHGVK
                     LGFMGFFVKACIHALHDIPSVNAEIDGDDIVYKNYYHIGIAVGTDRGLVVPVVRDADK
                     KSIAETEKEIAGFGRRARDGKLGLEEMQGGTFTISNGGVYGSLMSTPILNAPQSGILG
                     MHKTQERPVAIGGKIEIRPMMYLALSYDHRLVDGREAVTFLVRVKEAIEDPQRLILDL
                     "
     gene            3260..3688
                     /locus_tag="VNR65_04285"
     CDS             3260..3688
                     /locus_tag="VNR65_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ17930.1"
                     /translation="MGKQRLVPHLIVEGGLDAIDFYERAFGAEKVTVVMAEDGERVMH
                     AELEVNDGAFKLSDSFPEISNGMAAPGTAGAISVVIHLDFKKPKQIDEAIERAVKAGA
                     TLTMPAQDTFWGARYGRVRDPFGHVWALHALHKKKDPDEQ"
     gene            3737..>4725
                     /locus_tag="VNR65_04290"
     CDS             3737..>4725
                     /locus_tag="VNR65_04290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ17931.1"
                     /translation="MGDTYDLVVIGTGPGGYVCAIRAAQLGLKTAVVEKSRTFGGTCL
                     NVGCIPSKAMLHASELFEEAGHGFSAMGIKAKPELDLPTMQKYKQEGVDGNVKGVEFL
                     LKKNKVDTYQGTGRIAAPGKVIVQPVDGGEQKTLETKAIVIATGSDVARLPGMDIDEK
                     RIVSSTGALDLDRVPGHLLVVGAGVIGLELGSVWRRLGAQVTVVEFLDRIIPGMDTEV
                     AKQFQRILQKQGLEFRLGQKVAGVELNGAGPLKVQVQPSNGEGSKEVLEADVVLVAVG
                     RVPYTEGLGLEDAGVQIDNKRRIVIDDHFRTNVGGIYAIGDVVRGPMLAHKAE"
BASE COUNT          978 a         1382 c         1480 g          885 t
ORIGIN      
        1 cgataaaagt cggtcggagg ttctgccgct tctcctccac ggtgacgcgg cttttgctgg
       61 ccagggcgtg gtcgcagagt gcttcggcct gtccggcctg aaggggcacc gaaccggcgg
      121 ctcgcttcac ttcatcgtca acaaccagat cggcttcacg accgccccgc gtttctcgcg
      181 ctcatcgccc tacccgtccg acattgcccg tatgatcgag gcgccgatct tccatgtgaa
      241 tggcgacgat ccggaagccg tcgtttactg cgcgcgtgtg gcgaccgaat tccgccagcg
      301 attccacaag accgtcgtga tcgacatgat ctgctacagg cggcatggcc acaatgaagg
      361 cgacgagccg tccttcacgc agccgctcat gtacaagcgg atccgggagc agccgagcgt
      421 cgtctcgatc tattccaagc ggctcgccga agaaggcgtg gtcaaggagg ccgaggcaga
      481 gaagattcgc accggcttct gggacatgct cgaaaccgag ttcgaggcat cggaaggctt
      541 ccggccgaac aaggcggact ggctcgatgg gcgctggtca ggcatcacgc tcgccgagga
      601 aggaccgcgc cgtggcaaga ccggcgtcag catcgacacg ctgaagcaga tcggcgcaaa
      661 gctcaccgaa gtccccaagg gctttacgct gcacaagacg atcgacaggt tgcttgccaa
      721 ccggcgcaag atgatcggcg aagggcgcgg catcgactgg gctatggccg aagcgctcgc
      781 attcgggacg cttctcgagg aagatttccc cgtccggctt tccgggcagg acgtcgaacg
      841 cggtaccttc tcgcaacgcc attcggtgct caccgatcag ttgaacgagg agcagtatat
      901 cccgctgaac cacatctcga acaggcaggc tcgctttgag gtcatcaatt cgatgctctc
      961 cgaggaggcc gtgctggggt tcgaatatgg ctacagtctg gccgagccga acacgcttgt
     1021 ggtctgggag gcgcagttcg gcgatttcgc gaacggtgcg caggttgtga tcgaccagtt
     1081 cctggctagc ggtgagcgga agtggctgcg catgtctggc ctaatcctgc tcctgcccca
     1141 cggctatgag ggccaaggcc cagagcattc ctcagcgcgc ctcgagcgct atctgcagct
     1201 ttcggcggaa gacaactggc aggtcgcgaa ctgtaccacg cccgcaaact acttccacat
     1261 cctgcgccgt cagcttcatc gcggcttccg aaagccgctc atcctgatgt cgccgaaatc
     1321 gcttctgcgg cataagcggg tcgtgtcccc catcgaggat ttcaggccgg aaaccacctt
     1381 ccaccgcgtg ctttgggatc atgcccaact cggcgggagt gagaaggtga aactcgcgcc
     1441 ggatatggac atccagcgtg tcgttctctg ctccggcaag gtctactatg atcttttcga
     1501 ggaacgcgaa acgcgcggca atggcgagat ctatctcatg cgcttggagc agctctatcc
     1561 gttcccagcg cgcgcgctta tccaggaact gagccggttc ccgaacgccg aaatcgtctg
     1621 gtgccaggaa gaaccgaaga accagggcgc ctggacattt gtcgaagcca acatcgaatg
     1681 ggtgctcgat catatcggcg ccgactcgcg gcgtccgcgc tacgtcgggc ggcctgcctg
     1741 tgcttctacg gcggctggcc tcatgagcaa gcacacggct gaactccacg ctttccttga
     1801 cgaggcgttg acgctacaag gctagcactg gcgccgcggg gagtggtact ggagcgttaa
     1861 tctcgtctct ttcggatcgc gatatgaccc gttccttgcg gtcccttctt tcgatgcttg
     1921 cgaaggatct gcgcatggcc attgagatcg tcgttcccac gcttggcgag tccgtcacgg
     1981 aagccacagt cggaaaatgg ttcaagaagg cgggggatgc ggttaaggcc gacgaggctt
     2041 tggctgaact cgaaacggat aaagttacgc ttgaagtcaa cgctcccgct gcaggcgtca
     2101 tttcggagat tgtgatcaag gaaggcgaga ctgttgccgt cggcgcgatt ctcggctcga
     2161 tcgccgaggg acaagcggca gcagccgccc ccgcagtgaa ggcagcggaa aaaccgatcc
     2221 aggtgaagcc cgcaggtgcg ccgcaatcgg ttcaggcgtc accggctcct gaacagcgcg
     2281 ccagcagcgc gcagcaggca gcagcttcgc gctcgccgaa tgcggaaacg ctcgcccctt
     2341 ctgtgagacg catcgccgag gaaagcggca tcgatcccgc agccgtcacc ggttccggga
     2401 aggaaggccg cctgaccaaa ggcgacatgc tggccgttat cgagggcggt gcatcgaccg
     2461 gtggcgcgat aagctttgga aagctgcaag ccccgccccc atcggcgccc cgggcgccct
     2521 atgcgcctcc tcgcgaagcg ctggaagagc gggtccgcat gacgaagctg cgccagacga
     2581 tcgcccgcag gctcaaggaa gcgcagaaca ccgccgccat gctcaccacc ttcaacgagg
     2641 tggacatgac cacgattatg gaagtgcgta accgctacaa ggatctgttc gagaagaagc
     2701 acggcgtgaa actgggtttc atgggctttt tcgtgaaagc ctgcatccac gctttgcatg
     2761 acatcccgag cgtgaacgcc gaaatcgatg gcgatgacat cgtttacaag aattactacc
     2821 acatcggcat cgcagtcggc accgaccgtg gacttgtggt gcctgtcgtg cgggacgcgg
     2881 acaagaagtc catcgctgaa accgagaaag agatcgcggg cttcggaagg cgggcgcgcg
     2941 atggcaagct tggtctcgaa gaaatgcagg gcgggacctt caccatctcc aatggtggcg
     3001 tctacggctc gctgatgtcc accccgatcc tgaacgcgcc acagtccggc atcctcggca
     3061 tgcacaagac ccaggagcgg ccggtggcca tcgggggcaa gatcgaaatc aggccgatga
     3121 tgtatctcgc tctgtcttac gatcatcgcc ttgtcgacgg cagagaagct gttacgtttc
     3181 ttgtgcgcgt taaagaggcc attgaagatc cgcagcggct catcctcgat ctctaatctt
     3241 tttttggagg gaaagcgcca tgggcaagca gaggctcgtc ccgcatttga tcgttgaggg
     3301 cggtctcgac gccatcgact tctatgaaag agcctttggc gctgaaaagg tcacggtcgt
     3361 gatggcggag gacggcgagc gcgtcatgca cgccgagctt gaagtgaacg acggcgcgtt
     3421 caagttgtct gacagtttcc cagagatttc caacggcatg gctgctcctg gtaccgcggg
     3481 tgccatttct gtcgtaatcc atctcgactt caagaaaccg aagcagattg atgaggcgat
     3541 cgagcgggct gttaaagcgg gcgcaactct cacgatgccc gctcaggata cattctgggg
     3601 cgcacgatac ggccgcgtgc gcgatccttt cggacatgtc tgggcgcttc atgctctgca
     3661 taagaagaaa gacccagatg agcaataagc cgcacgcagc tggcgaaccg ctggctctcg
     3721 aacggagata tcagggatgg gtgacaccta cgatctggtc gtgatcggaa caggtcccgg
     3781 cgggtacgta tgcgcgatcc gggcggcaca gctcgggctc aagacagcgg tcgtggagaa
     3841 aagccggact ttcgggggaa cgtgcctcaa cgtcggctgt atcccctcga aagcgatgct
     3901 gcacgcgtcc gagcttttcg aggaggcagg ccacggcttc tccgcgatgg gcatcaaggc
     3961 gaagcccgaa ctcgatctgc ccaccatgca gaagtacaag caggaaggtg ttgacggcaa
     4021 cgtcaagggc gtcgagttcc tgctcaagaa aaacaaggtc gatacttatc agggcactgg
     4081 ccggatagct gctccgggaa aggttatcgt tcagccggtc gatggaggcg aacaaaaaac
     4141 tctggagacg aaggcgatcg tcatcgcgac gggctccgac gtcgcccggc tccccggcat
     4201 ggacatcgac gaaaagcgca tcgtgtcatc gaccggagcc ttggacctcg acagagtgcc
     4261 cggacatctc ctcgttgtgg gcgcaggggt tatcggcctt gagcttgggt cggtctggcg
     4321 gcggctcggc gcgcaagtga cggttgtcga gttcctggac cgcatcattc cggggatgga
     4381 caccgaagtt gcgaagcagt tccaacgtat tctgcaaaag cagggcttgg agttccggct
     4441 cggccagaaa gttgccggcg tcgaactcaa cggagccggg ccgcttaagg tgcaggttca
     4501 gccgagcaac ggcgaaggtt caaaagaggt tcttgaagcc gacgtggttc tggtcgccgt
     4561 tggacgagtc ccctacactg aggggcttgg gcttgaggat gccggcgtgc agatcgacaa
     4621 caagcggcgc atcgtgatcg acgatcattt caggacgaat gtcggcggca tttacgccat
     4681 cggcgacgtg gtgcgcggcc cgatgctcgc tcataaggcc gagga
//
LOCUS       DATMGX010000263         3348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_116672, whole genome shotgun sequence.
ACCESSION   DATMGX010000263 DATMGX010000000
VERSION     DATMGX010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116672"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /gene="arsB"
                     /locus_tag="VNR65_04295"
     CDS             <1..793
                     /gene="arsB"
                     /locus_tag="VNR65_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HWJ17932.1"
                     /translation="VLMFALAVIFLHDKPDYMVGLIMIGLARCIAMVIVWNELARGST
                     EYAAGLVAFNALFQVLFFSVYAWLFITVLPPFFGMSGSVIDISIKQIAESVLIYLGIP
                     CIAGVLTRVIMLRFVLKDWYHTHFVPKIGPITLIALLFTIIVMFSLKGKLIVSIPLDV
                     VRIAIPLLIYFVVMFVFSFFMSMRMGASYEKSATLSFTAASNNFELAIAVAVAVYGIN
                     SGAAFAAVIGPLVEVPVMIGLVNVSLWLRRKYFVSTSAQSPRQAA"
     gene            1184..2473
                     /locus_tag="VNR65_04300"
     CDS             1184..2473
                     /locus_tag="VNR65_04300"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HWJ17933.1"
                     /translation="MRWVDFLQYSAFLAVLTAPVRPLGGYLARVFTYERTFLDPLMTP
                     IEGLIIRLLGPAAERDMKWREYFLAFMAFGLVSVAIVFALLKFQQYLPFYGVVGTRFL
                     STPMTGDLAINTSVSFATTTTWQAYAGESTMSYFSQIVVLGVPWLIFFIHHISQAISV
                     NHIVDRIARETERVIDDIMPDCRPHGHDPSGVPYDDSKRETSVDSATSGYIRYFDTKR
                     LLELAKKFKVRIRLLRRVGQFVPEGVPLFSATREERLDKERVAELRSAFDIGPTRTLQ
                     QDAEFGVIQIVDIALKAISPAVNDPSTAITCIDQLGRIMIRFVNRSVPASVLCGPPHV
                     VRVIIPWIAVEDLLETAFEQIRHYSASDIAVSLRLLRAFDDIASTCSDPRIVESLVMG
                     GRRIVANCAKRLEPPQIEKLRGRLSALEARVTAAPET"
     gene            2812..3204
                     /locus_tag="VNR65_04305"
     CDS             2812..3204
                     /locus_tag="VNR65_04305"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWJ17934.1"
                     /translation="MDIQGERRLIASRDAVWRALNDLDVLREYAPSVLGSNSIVVLLT
                     DGLERGPPEELRAAAERLTRSCREVLWLNPLLRYADYAPLARGASVLAEILSPVRPAH
                     DPASFEALVRSLEALRRRGEPTRAARTP"
BASE COUNT          594 a          993 c          993 g          768 t
ORIGIN      
        1 ggtactgatg ttcgcacttg cggtgatttt tttgcacgac aaaccggatt acatggtggg
       61 cctgatcatg atcggtctgg cccgctgtat tgccatggtg attgtctgga acgagctcgc
      121 caggggctcc actgagtatg ccgccggtct tgttgcgttc aacgccctct ttcaggtttt
      181 gtttttcagt gtctatgcct ggctgttcat tacggtgcta ccgccgttct tcgggatgtc
      241 cggttcagtt atcgatatct cgatcaaaca gattgccgag agtgtcctca tttatcttgg
      301 catcccctgc attgccggtg tgctgactcg cgtgatcatg ctgcgctttg tcttaaaaga
      361 ttggtatcac actcattttg tgccgaagat cggaccgatc acgctgatcg cgctgctctt
      421 cacgataatt gtaatgttca gcctgaaggg caagctgatc gtcagcattc cgctggatgt
      481 ggtgcgcatt gccattccgt tgctgatcta ctttgtcgtg atgtttgttt tttcgttctt
      541 catgagcatg cgcatggggg ccagttatga gaagagcgct accctctctt tcaccgcagc
      601 gagcaacaac tttgaactgg ccatagccgt tgccgtggca gtttatggca tcaattcggg
      661 tgctgctttt gcggctgtca tcgggccgct ggtcgaagtg ccggttatga tcggcctcgt
      721 gaatgtctcg ctttggcttc ggagaaagta tttcgtttca acgtcagcac aatcaccgcg
      781 ccaagctgcg tgatctgctg cgcgagaaag gcacgcctat gcagagttcc agcttgccac
      841 cgctcactgt accgatgacc agctgatcga ctagatgctg gctcatccca tccaaacctg
      901 gcgcccaatt gcggtgacgc cgtggggcgt caaaatcagc caaccgtccg acggtattgg
      961 agatgtgccg atgagagtgg tggtgatccg cggtcgcacg caccgataag ctggtcgccg
     1021 gtagccgcca ttcagcttgt gcgccggatc gtgcgcggga aaaactaatg agtatttttc
     1081 gatctgccgc acctaagaga aattttcaca gcaataccat tccagatgcg gtgaaatggg
     1141 cgacatgccg ctgtggtcgg caacgatcag gcagtgagat ggcatgaggt gggtcgattt
     1201 tttgcagtat tccgcattcc tcgcggtgct gacagcgccc gttcgaccgc tcggcggcta
     1261 tctcgccagg gttttcacat atgaacggac ctttctcgat ccactgatga ctccgatcga
     1321 gggcctgatc attcggctcc tcggtccagc ggccgagcgg gatatgaaat ggcgggaata
     1381 ttttctcgcg ttcatggcgt ttggacttgt cagtgtggca attgtctttg cgctcctgaa
     1441 attccaacag tacctgcctt tctatggtgt tgtcggcacg cgcttcctgt cgactccgat
     1501 gaccggcgat cttgcaatca atacctccgt cagcttcgcg acgacgacga cctggcaggc
     1561 gtatgcgggc gaatcgacga tgagctactt cagccagatc gtcgtccttg gtgttccctg
     1621 gctgattttc ttcattcacc acatctcgca agcgatcagc gtcaatcaca ttgtcgatcg
     1681 gatcgcacgc gaaactgaac gcgtgatcga tgacatcatg ccggactgcc ggccgcacgg
     1741 ccacgatccg tccggcgtgc cttacgatga cagcaagcgg gagacctcgg tcgacagcgc
     1801 gacctccggc tacatccgct atttcgatac caagcgcctg ctcgagcttg cgaagaaatt
     1861 caaggtgcgg attcgtttgc tccgccgcgt tgggcaattc gttccggagg gtgtcccgct
     1921 gttcagcgcc acgcgggagg agcgcctcga taaggagcga gtcgcggagc tgcgttcggc
     1981 gttcgacatc gggccgacac gaacgctgca gcaggatgcc gaattcgggg ttatccagat
     2041 tgtcgacatc gccttaaagg caatctcccc ggcggtcaac gacccaagca ccgcgataac
     2101 ctgcatagac cagctcgggc ggatcatgat ccgattcgtg aaccgcagcg ttccggcctc
     2161 cgtgctatgc ggtccaccgc acgtcgtgcg tgtcatcatt ccatggatcg cggtggagga
     2221 tctgctcgag actgcatttg agcagatccg tcattactcg gcctcggata tagcggtcag
     2281 cctccggctg ctgcgcgcct ttgatgacat tgcatcaacg tgctccgatc cacgaatcgt
     2341 cgaatccctc gtgatgggcg gaaggcggat cgtcgccaat tgtgccaagc ggctggagcc
     2401 gccacagatc gaaaagcttc gcggtcggct cagcgcgctg gaagcccgcg ttacggcggc
     2461 gccggaaacg tgaaaaattc ccacgcggcc ggtgctgttg cctcggcgcc ggtctgcgcg
     2521 cgatgttaag aacagcgcta cgatgcgggc gttttcgccc gtgcgattgt caccccactc
     2581 cggaccagcc aacgacggac gccctattgc agagcgcggc cccatttacc gcgtgggact
     2641 ttgccacagg ttgccagcaa gctgccgttc gcatcgggca gctttgggtc tacgtcgaat
     2701 agcctgcttc ccgttggttc agagtgattt gcgcgacggc gccccgccgg ccgcagcgcg
     2761 cctcaatttg acttcaaccc cccgtccgga tcaggctcca gtggggggcg tatggatatc
     2821 caaggcgaac gacgtttgat tgccagtcgg gatgcggtgt ggcgcgccct caacgacctc
     2881 gatgtcctgc gcgagtacgc cccctcggtc ctcggttcga acagcatcgt cgtgctgctc
     2941 accgacggcc tcgaacgcgg cccgcccgag gaactgcgcg cagcggccga acgcctgacg
     3001 cgctcctgcc gtgaagtgct gtggctcaat cccctgctgc gctatgccga ctacgcgccg
     3061 ctggcgcgcg gagcgagcgt gctcgccgaa atactgagtc cggtgcgccc ggcacacgat
     3121 ccggcaagct tcgaggcgct ggtgcgatcg ctcgaagcgc tgcgccggcg cggcgagccg
     3181 acccgagcgg cccggacgcc gtgacgacac ccggccgccg gcttcgcgtc gcagccgcgc
     3241 tgcttgcgac cggcgtaatc gccggctgcg tggtttgggg cgggtgcgcg ctctggtatc
     3301 aggctccggg tggccccgca ttgaaaattt tgtgcatggt tctgtggg
//
LOCUS       DATMGX010000264         2070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_426171, whole genome shotgun sequence.
ACCESSION   DATMGX010000264 DATMGX010000000
VERSION     DATMGX010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426171"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /gene="lpxK"
                     /locus_tag="VNR65_04310"
     CDS             <1..636
                     /gene="lpxK"
                     /locus_tag="VNR65_04310"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWJ17935.1"
                     /translation="EQKFQPQLHILDDGFQHRSLVRDFDIVLMTEHDFEDSLLPFGRL
                     RESPSSLRRADATVLPEGFTGHNVSFQKLAIQTFARQGKPIWRMKRELVVPAVPSSPI
                     VFCGIARPQQFFAQIRATGITPAAEVEYRDHHSYCRGDIERLLAMRGQLGAGGFLTTE
                     KDAVNLGPLQSKLTPFAVAVLRLTLNDPTDAVEAILARTIFARITDRKPRS"
     gene            788..1720
                     /locus_tag="VNR65_04315"
     CDS             788..1720
                     /locus_tag="VNR65_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWJ17936.1"
                     /translation="MFGEISRVSREAPVLILKHRDRKVVPGGGANAIYNLADLGVNVL
                     PVGVVGDDEPGRLLLRAFRHKRIPVSGVFKDKNYATVTKTRILAGFAHTAGQQVVRLD
                     REPAEGPNSHLRRELVLAAREYTRASDALLVSDYGYDAATPTLLNVIREKRGIDKVPV
                     TVDSRHRMLEFSGATAATPNESEVEEVLGIRIGNDWERLLAAGDQIAGEMNLESLLIT
                     RGKDGMVVFPRRHKPVDIPIYGSDEVADVTGAGDTVIATFTAALAAGATAEEAAQLAN
                     YAGGIVVMKRGTATVNQQELLEALDKTPPAVREH"
     gene            1890..2070
                     /locus_tag="VNR65_04320"
                     /pseudo
     CDS             1890..2070
                     /locus_tag="VNR65_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
BASE COUNT          417 a          638 c          621 g          394 t
ORIGIN      
        1 gagcagaaat ttcagccgca attgcacatt ctcgacgacg gctttcagca ccgttcattg
       61 gttcgcgact tcgacatcgt actgatgaca gagcacgact tcgaggacag tctcttgcct
      121 ttcggacggc tgcgcgagtc gccatcctcg ttgcgacgcg cggacgccac cgttctaccc
      181 gagggtttca ctgggcataa tgtctccttt caaaaactgg cgatccagac tttcgctcgc
      241 caaggaaaac ctatctggcg aatgaagcgt gaactcgttg ttcccgcagt gccaagctcg
      301 ccgattgtgt tctgcggtat cgcgaggccg cagcagttct ttgcccagat acgcgccacc
      361 ggaatcacgc ctgcggcaga agtcgagtat cgcgaccacc attcatattg ccgaggtgac
      421 atcgagcgac tgctggccat gcgcggccaa ctcggtgctg gcggcttcct gaccaccgaa
      481 aaagatgcgg tcaatctggg gcccttgcag tcgaaactca cccccttcgc ggttgccgtg
      541 ctccgcctca cgctgaatga tcccactgac gctgtagaag ccattttggc gagaaccatt
      601 ttcgcaagaa tcactgaccg gaagccccgc tcgtgagaaa atcttggttg cgcccgatca
      661 ctaagcattg ggtgacaaca cattgatgac aaagcatatt aaagagaacg accgcctccg
      721 gaagatcgtc gagtcttttc ccaagctcac agtcaccgtc ctcgcggatc tggtcgccga
      781 cgagttcgtg ttcggcgaga tttctcgcgt ctcgcgggaa gctcccgtgc tgattttgaa
      841 gcaccgcgat cgcaaggtgg ttcccggcgg cggcgccaat gccatctaca acctcgccga
      901 cctcggagtg aacgtcttgc cggttggggt ggtgggcgac gacgaaccgg ggcggctgct
      961 gctgcgtgca ttccgccaca aacgcatccc ggtcagcgga gttttcaagg acaagaacta
     1021 cgccaccgtg accaaaacgc gcatcctggc tggcttcgcg cataccgccg gacagcaggt
     1081 cgtgcgcctg gatcgcgaac ccgccgaagg tcccaattca cacttgcgcc gcgaactcgt
     1141 tctggctgcc cgcgaataca cacgagcttc cgatgcgttg ctggtttccg actatggata
     1201 cgacgcggca accccaaccc tgctgaatgt gatccgggaa aagcgcggga tcgacaaggt
     1261 tcccgtgacg gtcgactcgc gccatcgcat gctcgaattc tccggcgcca cggcggcaac
     1321 cccgaacgag tctgaggtgg aagaagttct cggcatccgc atcggcaatg actgggagcg
     1381 ccttctggcc gccggagacc agatcgcagg cgagatgaat ctggaatcgc tgctgatcac
     1441 gcgcggcaaa gacggcatgg tggttttccc gcgacgccac aagccagtgg atattccgat
     1501 ctacggcagc gatgaagttg ccgacgtaac tggcgccggc gataccgtca tcgcaacgtt
     1561 taccgctgcc ctggctgcag gcgcgaccgc cgaagaggct gcccaactgg caaactacgc
     1621 cggcggcatc gtggtgatga agcgcggcac cgccaccgtc aaccagcagg aattgctgga
     1681 ggcactggat aagacgcccc cggcggttcg cgagcattag agcgtgtggg gacggggctc
     1741 tgccctgtcc aagcggggcg gcgccccgct tccacacgat cagatcggga tcattgagcg
     1801 cacaactcca tcagaaaatc gtttcgcgcg acgaactgca gcagcgggtg gccagttggc
     1861 ggcgcgccgg cgaccgcgtc acgctggcca acgggtgttt cgatgtgctg catgtcggcc
     1921 atgtgcgcta cctgcgtgcc gcgcgggaac tcggtggcaa attgattgtc gcggtcaacg
     1981 ccgatgcttc cgtacgcgca ttgaagggtg aggggcggcc cgtgataccg gcggaagaac
     2041 gcgcggaaat tctggcgtcg ctcgcggatg
//
LOCUS       DATMGX010000265         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_451581, whole genome shotgun sequence.
ACCESSION   DATMGX010000265 DATMGX010000000
VERSION     DATMGX010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451581"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VNR65_04325"
     CDS             <1..444
                     /locus_tag="VNR65_04325"
                     /inference="COORDINATES: protein motif:HMM:NF021926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HWJ17937.1"
                     /translation="AAGLDDGSLTPMSFAAHPVVVRVGGDYYCRSIQKVNPDGSLTFF
                     CAIDDGVVLTVARARDIVSSAEKELDRLDHETGGLDFVLAFECVLRRLDSEIHQVKHR
                     MCELYKKHNLVGFHTYGEQYNSMHLNQTLTGVAIGPKSASYVQRS"
     gene            428..1483
                     /locus_tag="VNR65_04330"
     CDS             428..1483
                     /locus_tag="VNR65_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWJ17938.1"
                     /translation="MSSGLKAETIAGANQELLDQIRKLTKINAVLMDRVERSVDRQGN
                     AFALFQTAIALEAQVRSRTEELTHVLRSLERTNAQLSAAKEDAEKANLSKTRFLAAAS
                     HDLLQPVISAKMSISTLSELQEIPEGKRLAQQVEGSLQTIEDLIRTLLDISKLDAGVI
                     VPEVRSFPLDDLLADLHATFESMMASTEIRLKVRRCGLVVESDPVLLWRVILNLVSNA
                     IHYTARGGVLVGVRRRGQGCHLDVVDTGAGIPECERQRIFDEFYRGASSASGNRAGLG
                     LGLSIVQRITQTLGHELSLKSSPGRGSRFRVTLPVVGTAKALSSPSLSALSDLAGVCV
                     LIVENDPAVAKAMQRLF"
     gene            1586..1837
                     /locus_tag="VNR65_04335"
     CDS             1586..1837
                     /locus_tag="VNR65_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17939.1"
                     /translation="MDGNDLGVTAIAAVRAILPGIPALVVTADHSPQTAEAARTVRAM
                     LLQKPISPGRLRASVSHLLAKAGGRLALSSLTAAVPKLS"
BASE COUNT          521 a          788 c          732 g          549 t
ORIGIN      
        1 gccgccggtc tcgacgacgg aagcctaacc cccatgagct tcgcagccca tcctgtcgtc
       61 gtcagagtcg ggggagatta ttattgccgc tcgatccaga aggtgaaccc tgatggctcc
      121 ctgacgttct tctgcgcgat cgacgatggc gtggttctaa cggtggcgcg cgccagggac
      181 atcgtgtcgt ctgctgaaaa ggagttggac aggctcgacc atgaaacggg cggcctcgat
      241 ttcgtgctgg ccttcgaatg cgtgctgcgc cgtctcgact ctgaaattca tcaagtgaag
      301 catcgcatgt gcgagcttta caaaaagcac aaccttgtcg gcttccacac ttatggcgaa
      361 cagtacaact ccatgcatct caaccagact ttgaccggag tcgccatcgg tccgaagagt
      421 gcgtcctatg tccagcggtc ttaaggccga gacgatcgcc ggggcaaacc aggagctttt
      481 ggatcagatc aggaagctga ccaaaatcaa cgccgtgctc atggatcgcg tcgagcgctc
      541 ggttgaccgc cagggaaatg ccttcgcact ctttcagacg gccatcgcgc tcgaggcgca
      601 ggttcgctcg cgcacggaag aattaaccca cgttctcagg agcctggagc gaaccaacgc
      661 gcagctttcc gccgcgaagg aggatgcaga aaaggcaaat ttgagcaaaa ccaggtttct
      721 cgcggctgcg agccatgacc ttctgcagcc cgtgatttcg gcgaagatgt cgatctcgac
      781 tctcagtgaa ctgcaagaaa tcccggaagg gaaacggctc gcgcaacaag ttgaaggatc
      841 gctgcagaca atcgaggacc ttatcagaac tctcctcgac atttcgaagc tcgacgcagg
      901 cgtcattgtc cccgaggtgc gctccttccc tctcgacgat ctcctggccg accttcacgc
      961 cactttcgaa agtatgatgg cttcgaccga aatcaggctc aaggtgcggc gctgcggcct
     1021 tgtcgtcgaa agcgatcctg ttcttctttg gcgggtcatt ctcaatctcg tttccaacgc
     1081 tatccactat acggcgcgcg gcggagtgct tgtcggcgtt cgccgccgcg gccagggctg
     1141 ccatctcgat gtggtcgata caggcgccgg tattcccgaa tgcgaacggc agcgcatttt
     1201 cgatgagttc taccgcggcg caagctcagc gagcgggaat cgcgccggac tgggacttgg
     1261 cctttcgatc gtccagcgca tcacccagac actcgggcac gagctttcgc tgaaatcatc
     1321 ccctgggcgc ggctcccgct ttcgggtcac gcttccagtc gtgggaactg ctaaggcgct
     1381 gtcctcgcca agcttgtccg cgctttccga cttggccggc gtttgcgttc tgatcgtcga
     1441 gaacgatccg gcggtcgcca aagccatgca gcggctcttc tagcaatggg gatgccgaac
     1501 cctcgttgct tacggcctct cggacgtcga caagcatctg aaaattgtcg aacgcctccc
     1561 cgacattgtc gttgccgatt accatttgga tgggaacgat ctgggtgtca cagcaatcgc
     1621 ggcggtgcga gcgattttac ccggcatccc cgcgcttgtg gttaccgccg atcacagccc
     1681 gcaaaccgcc gaggccgcgc gcaccgttcg ggccatgctg ctccaaaagc ccatctcgcc
     1741 cggccggttg cgggcaagcg tatcgcacct gcttgccaaa gccggaggga ggctcgctct
     1801 cagctctttg acggctgctg tcccgaagtt gagctagagg tttcgatgac cgccgacacc
     1861 tcggcgctgt tggcaacact tcggctcgga ccccgaattg ccccgccgcg ccagggcagt
     1921 gtggctttcc acgcgctggg tgtctgccgt cttcagtgac cggcctactt tgcggaactg
     1981 ccgcttccaa agtcgcttca gcgcagatcg tttagtcaaa attcaaaaag ggcgtcggcc
     2041 atcaccggaa ggtgcttctc gcactctgat gcggctgctt tcacggcttc gatctctttc
     2101 aacgcttgaa ccttctacag gcgcataaac gacccccggc cgttgaactg gaccgacatt
     2161 cgggttcccg ctatcgattg gctgttgata tgaccacctg actattgcgg aagcgcccgt
     2221 ggacagaacg gcaaggataa ttgctcgagc gaaacgcatg acaattttcc aaatacgtcc
     2281 aagtttacaa tggcggaggc ggtgaggacc ggttcgcgcg cgaacattcc aagaggcaca
     2341 gcttctgctt tggaggaaag cttagcatcc ttaaacgaac gctatgcttc ctgaaccggc
     2401 aaaagactga ggaacgcctt tacggagcgt tagtgacgac cgcgttaccc ggctttgaaa
     2461 cacgcgctgc taccatccag ggctgccgcc tgctctggcc gatggttccg gcggcatctg
     2521 agcggccacc ctcaactctt tgacggctgc tgccctagaa atgcatcgaa gttgagatgc
     2581 gaggtctcga
//
LOCUS       DATMGX010000266         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_347959, whole genome shotgun sequence.
ACCESSION   DATMGX010000266 DATMGX010000000
VERSION     DATMGX010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347959"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /gene="lepA"
                     /locus_tag="VNR65_04340"
     CDS             complement(<1..1484)
                     /gene="lepA"
                     /locus_tag="VNR65_04340"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWJ17940.1"
                     /translation="MTTVPTTQDAAPRDPASKGVTPLAHIRNFSIVAHIDHGKSTLAD
                     RLIQLCGGMSDREMAGQAQVLDSMEIEKERGITIKAQTVRLDYRAKDGELYQLNLMDT
                     PGHVDFGYEVSRSLAACEGSILVVDASQGVEAQTLANVYKALDNNHEIVPILNKIDLP
                     AAEPDRVKQQIEDVIGLDAHDAVEISAKTGLNVDQVLEAIVTRLPPPKGDVKAPLKAM
                     LIDSWYDPYLGVVVLVRVVDGLLKKGMRIKLMSTGAVHPIDRVGIFRPKQEMLDVLGP
                     GEVGFFTAAIKEVADTRVGDTVTEDKRQAAQALPGFKPVQPVVFCGLFPVDASQFEDL
                     RDAMSKLRLNDASFQYEMETSAALGFGFRCGFLGLLHLEIVRERLEREFNIDLITTAP
                     SVIYHLYMTDGQKIEMHNPADMPDVVKIDRMEEPWIKATIFVPDEHLGAVLKLCEDRR
                     GRQKELTYAGSRAMVVYDLPLNEVVFDFYDRLKSISRGYASFDYT"
     gene            complement(1597..>2004)
                     /locus_tag="VNR65_04345"
     CDS             complement(1597..>2004)
                     /locus_tag="VNR65_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HWJ17941.1"
                     /translation="KKKIALGAGGNGQATSESPVRTVGAVSMLARTVHGIEPKDLRSL
                     VDDGKRQIKSGIVAIVGVTEEGKAGIVVGVTDDLTAKYSAVDLVKAAAAALGGQGGGG
                     RPDMAQAGGPDGAAAEAALDAIASRLALAAAAA"
BASE COUNT          368 a          636 c          591 g          409 t
ORIGIN      
        1 gtatagtcga agctcgcata gccgcggctg atggatttca gcctgtcata aaaatcgaac
       61 accacctcgt tcagtggcag gtcgtagacc accatggcac ggctgcctgc ataggtaagt
      121 tctttctgcc tcccgcggcg atcttcgcag agcttcagga ccgcgccgag atgctcgtcg
      181 ggcacgaaga tcgtggcctt gatccaaggc tcttccatgc ggtcgatttt cacgacatcg
      241 ggcatgtcgg cggggttatg catctcgatc ttctgcccgt ccgtcatata aaggtgataa
      301 atcacgctcg gagcggtcgt gatgaggtcg atgttgaact cgcgctccag gcgctcgcgg
      361 acgatttcga ggtggagcaa gccgagaaag ccgcagcgga agccgaaacc gagcgcggca
      421 ctggtctcca tctcatattg gaagctcgcg tcattgaggc gaagcttcga catggcgtcg
      481 cgcaagtcct cgaactgaga ggcatccacc gggaacagcc cgcaaaacac cacgggttgc
      541 acgggcttga accccggaag cgcctgcgcg gcctggcgtt tgtcctcggt gaccgtatcg
      601 ccgacgcgcg tgtcggcgac ttccttgatg gccgcggtga agaagccgac ctcgccaggc
      661 ccgagcacgt ccagcatttc ctgcttaggc cggaagatac caacccggtc gatggggtgc
      721 accgcgccgg tggacatcaa cttgatgcgc atgccctttt tcagaaggcc atccaccaca
      781 cgcaccagca ccaccacgcc gagataaggg tcgtaccagc tatcgatcag catggctttg
      841 agcggtgcct tcacgtcgcc cttcggcggc gggaggcgcg ttacgatcgc ttccagcacc
      901 tgatcgacgt tgaggcccgt tttggcggaa atctccacag catcatgcgc gtcgaggccg
      961 ataacgtcct cgatctgctg tttgacgcgg tcaggctcgg ctgcggggag gtcgatcttg
     1021 ttgaggatcg gcacgatctc gtgattgttg tcgagcgcct tgtaaacatt ggcaagcgtc
     1081 tgcgcctcga cgccctggct cgcatcgaca acaaggatcg aaccctcgca cgcggcaagg
     1141 ctccggctca cctcatagcc aaaatccacg tggccgggcg tgtccatcag gttcagctga
     1201 taaagctcgc catccttcgc gcgataatcg agccggaccg tctgagcctt gatcgttata
     1261 ccgcgctctt tctcgatctc catgctgtcg agaacctgag cctggcccgc catctcgcga
     1321 tcggacatgc cgccgcaaag ctgaatgagc ctgtccgcga gcgtcgattt gccgtgatcg
     1381 atgtgcgcga cgatcgagaa atttcgaatg tgcgcgagcg gcgtcacgcc ttttgaggcg
     1441 gggtcgcggg gcgcggcgtc ttgcgtggtc gggacagtgg tcatggcgcc ttacataatg
     1501 gaaaacagga tcggctggaa gcgcgctctc ttaaggcgca gcctccgcca tggccggttt
     1561 atgctaagtc cagttcctcc cgcggaagaa ctggtttcaa gccgcagctg ccgcgagcgc
     1621 gaggcgactc gcaatggcgt cgagcgccgc ctctgcggcc gccccatctg gaccgcccgc
     1681 ctgagccatg tccggccttc cgccgccgcc ttggccgcca agcgctgccg cggccgcctt
     1741 gacgaggtcc accgcactat attttgccgt caaatcgtcg gtgacgccaa caacgatccc
     1801 ggcctttccc tcctcggtca cgccgacaat ggcaacgatg ccggacttga tctgccgctt
     1861 accgtcgtcc acgaggctgc gaaggtcctt tggttctatc ccatgcacag tgcgggcaag
     1921 catcgagacg gctcccactg tgcgaaccgg gctctcgctc gtcgcttgac cgtttccgcc
     1981 agctccgagt gcaattttct tttt
//
LOCUS       DATMGX010000267         2348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_260823, whole genome shotgun sequence.
ACCESSION   DATMGX010000267 DATMGX010000000
VERSION     DATMGX010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260823"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VNR65_04350"
     CDS             <1..883
                     /locus_tag="VNR65_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWJ17942.1"
                     /translation="QVFPEHKFHIVDVLQQRGHIVGMTGDGVNDAPALKKADCGIAVS
                     GATDAARAAASIVLLTPGLSVIIDAIKESRKIFQRMNSYAIYRIAETLRVLFFMTLAI
                     LIFNFYPLTAVMIVMLALLNDGAILSIAYDNVHYKDKPEAWNMHMVLGVATVLGVIGV
                     VSAFGLFYLGERVFYLDRAHIQTLMYLKLSVAGHLTIFLTRTRGPFWSIRPARILLIA
                     VFGTQALATLIAVYGLFMTPLGWGWALFVWGYALAWFLVNDRVKLLAYRIFDPAKATT
                     LPDLKPAAFLTGDDATR"
     gene            913..1782
                     /locus_tag="VNR65_04355"
     CDS             913..1782
                     /locus_tag="VNR65_04355"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HWJ17943.1"
                     /translation="MKIDSKDFRVKEGDEVNLKKWPTAIDPVYESKEHYHELLREHVA
                     HLSSQQQLLYASSSHAILLIFQAMDAAGKDGVIKHVMSGVNPQGCQVFSFKHPSPAEL
                     QHDFLWRTTRDLPERGRIGIFNRSYYEEVLIVRVHPEILRNEGLPDAPHDDKTVWRER
                     YRSIANLERHLHGNGTRIVKFFLHLSKEEQRKRFLQRIDEPEKNWKFSLADVEERKFW
                     KQYMKAYEECLSATSTKDSPWYVVPADDKENARLIVSQIVLDTFEGLKMTYPKTSAER
                     WQELQSIREQLKT"
     gene            1947..2186
                     /locus_tag="VNR65_04360"
     CDS             1947..2186
                     /locus_tag="VNR65_04360"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWJ17944.1"
                     /translation="MASPIILLIAKLVFFALLLLLTAMNRFWLVPALMARDSQQRNAK
                     TLLQLRYHVMSEQLLGLVIVAIVSFLGSIAPEGGG"
BASE COUNT          512 a          656 c          655 g          525 t
ORIGIN      
        1 ccaggtattc ccagagcata aattccacat cgtcgatgtg ctgcagcaac gcggtcacat
       61 cgtcggcatg acaggcgatg gggtaaatga cgcccccgcg ctgaagaagg ctgactgcgg
      121 catcgctgtt tccggcgcga cagacgcagc gcgcgcggcg gcgtccatcg tgcttttaac
      181 ccccggcctg tcggtgatta tcgatgcaat caaggagagc cggaaaattt tccagcggat
      241 gaatagctac gcgatctatc gcatcgccga gacgcttcgc gtgctgtttt tcatgacgct
      301 ggcgatcctg atcttcaatt tttatccatt gacggcggtg atgatcgtga tgctggcgtt
      361 gctcaacgac ggcgccatcc tgtccatcgc ttatgacaat gttcactaca aggacaagcc
      421 cgaagcctgg aacatgcaca tggtgctggg ggtcgcgacc gtgctgggcg tcatcggcgt
      481 ggtgtccgca tttggactat tttatcttgg cgagcgcgtc ttttacctcg accgcgcgca
      541 tatccagacg ctgatgtatc tcaaactttc cgtggcgggt catctaacga tttttctgac
      601 gcgcacgcgc ggcccgttct ggtccattcg ccccgcgagg atcctcttaa tcgcggtgtt
      661 cggtacgcaa gcgctggcca ctctgattgc tgtgtacggg ctgtttatga caccgctcgg
      721 ctggggttgg gccttgttcg tttggggcta tgctctggcg tggttcctcg tgaacgatcg
      781 cgtgaagctg ctcgcctatc ggattttcga tcccgccaaa gccacgacat tgcccgattt
      841 gaagccggcg gcgtttctga cgggcgacga cgcaacgcga tgaacacggc cagcatcgcc
      901 gaaaggataa aaatgaagat cgattcgaaa gattttcggg tgaaggaggg agacgaggtc
      961 aatctcaaga agtggccgac ggccattgat cccgtgtacg agtcgaagga gcattaccat
     1021 gagcttttaa gagaacatgt tgcgcatctc agctcccagc agcagctgct ttacgcatcc
     1081 agcagccatg ccatcctcct catctttcag gcgatggatg cggccggaaa agatggcgtg
     1141 atcaagcatg tgatgtccgg ggtcaacccg caaggctgcc aagtgttcag cttcaaacac
     1201 cccagccccg ccgaattgca acacgatttt ctctggcgca ccacgcgcga tctccccgaa
     1261 cgggggagga tcggcatctt caaccggtcc tattacgagg aagtgctgat cgtccgggtg
     1321 catcccgaga tactccgcaa tgaagggttg ccggacgcgc ctcacgacga caagacggtc
     1381 tggcgcgagc ggtatcgttc gatcgcgaat ttggagagac atctccatgg caatggaacc
     1441 cgcatcgtga agttcttcct ccacctctcg aaggaggagc agcgaaagcg cttccttcaa
     1501 cgaatcgatg aaccggaaaa gaactggaaa ttcagccttg cggacgttga ggagagaaaa
     1561 ttctggaagc aatacatgaa ggcctacgag gaatgcctta gcgcgacaag caccaaggac
     1621 tcgccctggt atgtcgttcc cgcagacgac aaagagaacg cgcggctgat tgtatcccag
     1681 attgtgctcg ataccttcga aggactcaaa atgacttatc caaaaaccag cgcggagcga
     1741 tggcaggaat tgcagtcgat ccgtgagcag ctcaagacgt gagtcttaag tgcgtcgtcg
     1801 caagaagatc gtgatcagaa gatgacgatg tataccaata tcttaatacc aacggatgga
     1861 tcggaactct ccgggaaggc tgtccagcac ggcatgcatc gccctggcca agtcgatcgg
     1921 tgccaaaatc atcgttccgc aggctgatgg cctccccaat aattctcctt atcgcgaagc
     1981 tcgtattttt tgctctcttg ctgcttctta ccgccatgaa cagattttgg ctcgtgcccg
     2041 ccttaatggc cagggacagc cagcagcgaa atgcgaaaac cttgttacag ctccgctacc
     2101 acgtcatgag cgaacagctt ctcggcctgg tgattgtcgc cattgtaagt tttctcggct
     2161 ccattgctcc tgaaggcggc ggataagtgt tggccccccc aacggcgacg cgtgctggta
     2221 cacgcgcttc gaatctcggc ttctcagccg cgtttcggat cgtctcctcc ggcaggcggc
     2281 gttttctttg acgatcgtca acgcggcctg actggcaacc ggtgaacgtg aatctggcga
     2341 cgcctttg
//
LOCUS       DATMGX010000268         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_330635, whole genome shotgun sequence.
ACCESSION   DATMGX010000268 DATMGX010000000
VERSION     DATMGX010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330635"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..843)
                     /locus_tag="VNR65_04365"
     CDS             complement(415..843)
                     /locus_tag="VNR65_04365"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HWJ17945.1"
                     /translation="MNTFCNSMTRRALIATATLLVMSGSALAVDVKVKLTGAEETPPV
                     TTSASATGTIVIAADKSVSGTLMSSGMDGTVAHIHVGAPGQSGPPIITLIKGPDGAWS
                     VPEGSKLTDDQYASFKAGNLYVNIHSADHKPGEIRAQLKP"
     gene            1183..1599
                     /locus_tag="VNR65_04370"
     CDS             1183..1599
                     /locus_tag="VNR65_04370"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ17946.1"
                     /translation="MESNYLSRDTYGMYKNVGPGPGPALMGADTLIGNDVYNMAEESL
                     GSIKELMIDMSSGKINYAVLSYGGFLGMGDRLFAVPWQALKLDAVNKRFTLNASKEQL
                     KNAPGFDKDHWPSMADNTWASDVHKFYGVKTTSRVQ"
     gene            1799..>2208
                     /locus_tag="VNR65_04375"
     CDS             1799..>2208
                     /locus_tag="VNR65_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWJ17947.1"
                     /translation="MGSTANRIKDIGPQPQSFDIERATKENSNYRSVAWSGRYLQVTL
                     MSIPAGGDIGLEAHPQTDQFLRLDAGSDRVQMGAAKDKLTFEKEVSNGWCVLVPAGTW
                     HNITNTGTTPMQVYAIYAPAHHAPDNVQATAAAA"
BASE COUNT          521 a          581 c          626 g          480 t
ORIGIN      
        1 gatggggcgc cgcgtgttgt cttgagtgaa gccgaaaacc gaaacttcct cgatgccgag
       61 cgtcttacat gcttcgaaga gcgccactcc cggggcaatg ccgttctcat accccttttc
      121 cttcgcaagc ccgttttgca cggcccatcg tcggttaccg tcgggaatga agcccacatg
      181 acgagggacg cgtcgtcggg gctgaagcgg gcgattttcg gaaaccggga ttagctgagc
      241 actcattgaa ggaattttta accccgatcg tattggactg atttagcgaa attcaacgtc
      301 actctagacc cgtgcacatt gttggggttc aatttttaat gcagcggcca cgcccagccc
      361 cgagcgagtc acaagcggtg ctacgcagaa ccagcaaccc acgcgcgatc gtccttaagg
      421 cttcagctga gctcgaatct cgccgggctt gtgatccgca ctatggatat tcacgtataa
      481 gttacctgcc ttgaaactcg catactgatc atcggtgagc ttggaacctt cagggaccga
      541 ccaggcgcca tcgggacctt tgatgagtgt gataattgga gggccacttt gtcctggcgc
      601 gccgacatga atgtgcgcca cagttccgtc catgccgctg ctcatgagcg tgccgctcac
      661 ggacttgtcg gcagcgatga caatggtccc agtcgcggac gccgacgttg tcaccggcgg
      721 agtctcttca gcgccggtca atttcacttt cacatcgaca gctaacgcgg atccactcat
      781 tacgagcagc gtcgctgtcg cgatcagtgc gcgacgggtc atactgttgc aaaatgtatt
      841 catgggtaac tcctataggt ttctgctgtg aactctgatt gtagtctcac tgcgacaaaa
      901 tgggtgctta agtaatccga ccgctatgtg cctgaagaag gcttcgcgaa ccggtcgcat
      961 tgccgttaat aggtgcgcct ggctgaacaa aggcacgtaa cagtgtacgc caacgtaccg
     1021 acaaatacgg aatttgccat tatgatcgaa tggtgtcggg ggtgaattga ttcgccgaca
     1081 tcgaatgatt tgccgggtgc ccccgcacac aaatcaaggc agcgatagtg agcacacaat
     1141 cgcagttcga ggtttgtcaa cagttaacta tcggagataa ttatggaatc caattacctt
     1201 tcacgcgaca cctacggcat gtacaagaac gtggggccgg ggccgggacc cgcgctcatg
     1261 ggagcagata cgctgatcgg aaacgatgtg tacaacatgg cagaggaaag cttgggctcg
     1321 ataaaggagc tcatgattga tatgagttca ggaaaaatca attatgccgt actgtcttac
     1381 ggtggttttc ttggcatggg agatcgttta ttcgcagttc cgtggcaagc gctcaagctt
     1441 gacgcggtca ataagcgatt tacgctaaat gcctccaaag agcagctgaa gaacgcgccg
     1501 ggttttgata aggatcattg gccttcgatg gccgacaaca catgggcgtc ggatgtgcac
     1561 aaattctacg gcgtgaaaac gacgagtcga gttcagtagt cgaaatcggt aactcagccg
     1621 gggctggcga ttgcgctagc cccgtggcca aacagtcacg gcatggctga gtaggaatgg
     1681 cattttgccg tccggatctc cgcgaaagac atcgcagcgc caagagtggg cgaggtttgg
     1741 gcgtccaggt ttgggcgtta cgtaagaact gttatcgcaa tcattgagga gacccgacat
     1801 gggcagcact gctaatagga ttaaagacat tggaccacag ccgcaatcgt tcgacattga
     1861 acgcgcgacg aaggagaatt caaattaccg ttcagtcgct tggagcgggc gatatttgca
     1921 agtgacgctg atgtcgatcc ccgccggcgg cgacattggc cttgaagcgc atccgcagac
     1981 cgatcagttc ctgcgcctcg atgctggcag cgaccgagtg cagatgggag ccgctaagga
     2041 caaactgact ttcgaaaagg aagtttcaaa cggctggtgc gtcctcgttc cggcggggac
     2101 ctggcacaac atcaccaata ctggaacgac gcctatgcag gtttacgcga tctacgcgcc
     2161 cgcacaccac gcacctgaca acgtgcaggc gacggccgcg gcggccga
//
LOCUS       DATMGX010000269         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_284736, whole genome shotgun sequence.
ACCESSION   DATMGX010000269 DATMGX010000000
VERSION     DATMGX010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284736"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VNR65_04380"
     CDS             <1..223
                     /locus_tag="VNR65_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17948.1"
                     /translation="RVSQFSTSAVVSIIPAAWWATGPVARRNGGEAVDGASESNRKGD
                     SRRNASDSIYAIGVAVFHQLDDEALMGGG"
     gene            complement(648..815)
                     /locus_tag="VNR65_04385"
     CDS             complement(648..815)
                     /locus_tag="VNR65_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17949.1"
                     /translation="MNTEHQASIDHLREELCAMIMAQMEDLQNQIDQLRKEMQMLKER
                     NERLESMARRQ"
     gene            complement(836..1162)
                     /locus_tag="VNR65_04390"
     CDS             complement(836..1162)
                     /locus_tag="VNR65_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17950.1"
                     /translation="MTPITDLLLRRREKPTRAGYVYEVLQDGKVLLASINPEFAACRL
                     LASEGVTGKARFWREGRPEHDIEFDIDRAAKFTVRENEQSGPRFVKFKPFVVQAEYVP
                     QFAAAA"
     gene            complement(1375..2016)
                     /locus_tag="VNR65_04395"
     CDS             complement(1375..2016)
                     /locus_tag="VNR65_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWJ17951.1"
                     /translation="MLLGYMRVSKADGSQVLDLQRDALIAAGVEPDDIYEDMCGGSKD
                     DRPGLAACLKALRRDDVLVVWRLDRLGRNLPHLIETVEDLTKRGVGFKCLTGVDIDTR
                     TAAGRLVLTIFAGLAQFERDLIRERTIAGLEAARARGNVGGRISALSPSKLRRAQVAM
                     AHRDTSVAALCAELGVSRTTLYRNITPDGQLTKVGQRALEDKRSRKTGKRATA"
BASE COUNT          439 a          616 c          674 g          466 t
ORIGIN      
        1 ccgggtatca caattttcga cgagcgcagt cgtgtcgata attccagcag cttggtgggc
       61 aactgggcct gtcgccagac gaaatggcgg cgaggctgtc gatggtgctt ccgaaagcaa
      121 tcgaaaaggc gacagccgaa ggaacgcgtc tgacagcatc tacgcgatcg gggtggcggt
      181 atttcaccag ctcgacgacg aagcactgat gggcggcggc taaacgcaaa agcccctagc
      241 gatgggacgc taggggcttg ggatggggaa atatatccgc gcaatacatc ttggaaggaa
      301 cgggcagagc aaggtgcttc gatccccgct ctgctgcaga taatggtatt ggctgcgtgg
      361 atgcaatgca ttggcttttc gcacgcagag cctgagatac cggggaatag gtgcagcgaa
      421 aacacccgtg ggcggatgtt atccagagag tggtcgttga cccgcagacg tggcgcccgc
      481 gcaactggga ggcttgtcca cccactcttc gcgaggttca ggattggcga gcgtcgatga
      541 gaaggatggc cgagcgtcgc attcgcgtcc ttccctcagc cgaccatctc tttcgcatga
      601 gggacgcaga agctcgcttc aaagagatcg gggtgctggt gtagcagtca ttgcctgcgg
      661 gccatggact ccagcctttc atttcgttct ttcaacatct gcatttcctt acgcaactgg
      721 tcgatctgat tctgcagatc ctccatctga gccatgatca tggcgcaaag ttcctcgcgc
      781 aaatggtcga tgctggcttg atgttctgtg ttcattgtgg tctccaataa gatgatcagg
      841 cagcggcggc gaactgcggc acgtattcgg cctgcacaac gaacggctta aacttcacga
      901 agcgcggtcc gctttgctcg ttctcccgca cggtgaactt cgcggcgcgg tcaatgtcga
      961 actcgatatc gtgctccggc ctgccttccc gccagaagcg ggctttgccg gtgacgccct
     1021 cactggccag gaggcggcag gccgcaaact cagggttgat cgaggcgagc aagacctttc
     1081 cgtcttgcag aacctcataa acgtagccgg cgcgggttgg cttttccctg cggcgcagca
     1141 gcaagtcggt aataggcgtc atcggcgtta gaacctctct aggatttgtg gggggtggta
     1201 gattttgccc ggttgggcat cgcccggtac gttggagcgg gcgaggcttt cgcctgtgta
     1261 cgtatgggac gtaaaaaacc tgaagggggc ggccggagtg atgacagacg acggatggga
     1321 tccttgtgat gaggcagcgc acccggcatc gccgacgatg cgcgcctgga tgcgttatgc
     1381 ggtggctcgc ttgcctgtct tccggctgcg cttgtcttcc aatgcgcgct ggcccacctt
     1441 ggtaagttgc ccgtcaggcg tgatgttgcg atagagcgtt gtgcggctga cgcccaactc
     1501 ggcgcatagg gcggcaacgc tggtgtccct gtgtgccatg gcaacctgtg cgcggcgtaa
     1561 cttggatgga ctgagagccg atatgcgtcc gcccacattg cctcgtgcgc gtgccgcctc
     1621 aagcccggcg atggtgcgtt ccctgatcaa gtcacgctcg aactgagcaa gcccggcgaa
     1681 gatcgtgagc accagccgcc ctgctgccgt ccttgtgtcg atatcgacgc cggtcaggca
     1741 cttgaagccc acgccacgct ttgtgaggtc ttcgacagtt tcgatcaaat gcgggagatt
     1801 gcgcccaagc cggtcgagcc gccacaccac aagcacgtca tccctgcgca gcgccttgag
     1861 gcaggcagcc aagcccggcc gatcatcctt tgagccgccg cacatatcct cgtagatgtc
     1921 gtcaggctcg acaccggcag cgataagcgc atcgcgttgt agatcgagca cttgcgaacc
     1981 gtcggccttg ctcactctca tgtatcccag cagcatgaaa aatccccata tttcgtagtc
     2041 acgataacat gcgtattcgg gacagtatag cagggaaggc ttgatctgcc taaagaatgt
     2101 tacgttggcc gtaccgaaat caaagtcacg cgaacggatg ggattgtggg attcaggtgc
     2161 tcgagcttgt cgccgtcgaa ataaaccagc cgctt
//
LOCUS       DATMGX010000270         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_84967, whole genome shotgun sequence.
ACCESSION   DATMGX010000270 DATMGX010000000
VERSION     DATMGX010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84967"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1327)
                     /locus_tag="VNR65_04400"
     CDS             complement(44..1327)
                     /locus_tag="VNR65_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ17952.1"
                     /translation="MTKKGGFAAILGAALLAAIIAPAPLLAETGTPVGQLVAETGSTA
                     QVASAYGQGVADAFAYINRLGGVSGKPISLDTVDYGYDLPRALSTYKTWKENLKPVAI
                     QGWGTADTEALVDSVTKDAIVFMSGSSSGHLTDPIGRSPWTSTPAPYNFFYGPSYSDG
                     CRGLVQWAAEEWRRSGAASRSTFLGDLDRPKFVFMGDNHPYPNSPKAACTDYARSLGF
                     EVLPPVRYSLSPADFSTQCQSLKTSGAHYAFLANTAESNAKLVKDCAGAGVSTQFLTN
                     IYGWDEGTAAQAGTAGNGMVWVVSVAPWDSDVAGMKTVREISKISDPSAEATRPVHYI
                     RGICSAYLMRDAMLTAGNAGAITGPGIKKALEEMRDHVPAGLEGVCLPTTFTPVDHRG
                     TTTVNLYRNDFNFASSHAQKIYTTTVPLRPDWLGW"
     gene            2075..>2395
                     /locus_tag="VNR65_04405"
     CDS             2075..>2395
                     /locus_tag="VNR65_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HWJ17953.1"
                     /translation="MSILIVVTSNDRLGDTGKPTGVWLEELATPYYIFRDEGVKVVLA
                     SPKGGKPPIDPRSEEPQAQTDSTKRFFKDKEAQQAFENTAQLSTINPGDYGAVFYPGG
                     HGPLW"
BASE COUNT          479 a          699 c          731 g          486 t
ORIGIN      
        1 cctcctagca ccaagtgcga aatgatgaac tctcggaagg cggctaccag ccgagccagt
       61 ctgggcgtaa cggcaccgtc gtcgtgtaga tcttctgcgc gtgcgaactc gcgaagttga
      121 agtcattgcg gtaaaggttc acggtcgtgg tgccgcggtg atctaccggc gtgaaagtgg
      181 ttggaagaca cacgccttcg agccctgccg gcacatgatc gcgcatttcc tccagagcct
      241 ttttgatgcc cggccccgtg atcgcgccag cgttgccggc ggtcagcatc gcatcccgca
      301 tgaggtaggc cgagcaaata ccgcggatgt aatgaacagg ccgggtagcc tcggcgctcg
      361 gatccgatat tttggagatc tcgcgaacgg tcttcatgcc cgctacatca ctgtcccaag
      421 gtgcgacgct tacgacccag accatgccgt tgccggcggt cccagcctgg gccgcggtac
      481 cttcgtccca gccgtagatg ttggtcagga attgggtcga gacacccgcc ccggcacaat
      541 ctttgacaag tttggcgttc gactccgccg tgttggcaag gaaggcatag tgcgcgccgc
      601 tggtttttag cgattggcat tgcgtggaga agtcagccgg cgacagcgag tagcggaccg
      661 gcggcagaac ctcgaatccg agactgcgcg cgtaatccgt gcaagcggcc ttcggtgagt
      721 tcggatatgg atggttgtca cccatgaaca cgaatttggg ccggtcaagg tcgccaagga
      781 atgtcgagcg gctggcggct ccggagcggc gccactcctc agcggcccac tggaccaacc
      841 cgcggcagcc gtccgagtag gatggtccgt agaagaagtt gtagggggcg ggagtgctgg
      901 tccaaggact gcgcccaatc ggatcggtca ggtggccaga cgatgatccc gacatgaaaa
      961 cgatggcatc cttggtgacg ctgtcaacca aggcttcggt gtcggcagtg ccccagcctt
     1021 ggatggcaac cggcttcagg ttctctttcc atgtcttata ggtgctgagc gcacgcggca
     1081 aatcgtagcc atagtcgaca gtatccagcg agatgggctt cccactgacg ccaccaagcc
     1141 ggttgatgta agcgaaagcg tcggcgacgc cctgcccata ggccgaagca acctgcgctg
     1201 tgcttccggt ttccgcgaca agctggccga ccggcgtgcc ggtctctgcc agaagcgggg
     1261 ctggtgcgat aatggcggct aaaagcgccg cgccaaggat tgcggcaaag ccgccctttt
     1321 ttgtcatgag gtctcctccc ggtttgtaca gctgggcatt gctctgcctc taacgttccg
     1381 tctgcgcttt catttttacc ctctttggag gaattcgcct agcgtgatag tcgcgcaggc
     1441 gagccaagcc tagatcgtgc ggcctggagc ggggagcgtg gaatttggaa ctgtttttgg
     1501 cggccgcccg cgcgcttgca gcgtttctcg tcgctgcgcc ctggctttac cagacttcga
     1561 gttgacgcct gcggggtata cggttaaggg cgtggacgca tcccaccatc aggcgagata
     1621 gactggcgag cgctgcgtca aagtggcgtc cgttttgcct acaacaaggc aacggagggc
     1681 gcgagcctgc gcgatcccca ctttggcgag agctggcagc gctcgcatga tccccgagtg
     1741 gcgcgcgggg cctaccactt tttctcggtg cacaaatcgg gagcggtgca agctgcaaaa
     1801 tttcattgct gcaaaaacaa cgcgagagcc gcagccataa tcctgaggag catcgcggcg
     1861 caccttccgc ctggcacccg ctccaggagc caagttgcca ggtgccgcga ggagaactct
     1921 cgaaaaacgt cctcaatata aaaagacgag ctgaaaattg gatcgtccgt ttagcccgtc
     1981 cacatcggcc cctatatgtt tgctggtgtg aaggtcgtta gaagaagctt ccgcttcatt
     2041 caatggatct cgttcttatg tcaggaggcc ccgcatgagc attcttatcg tagtaacctc
     2101 caatgatcgc cttggcgaca ccggcaagcc gaccggtgtt tggctcgagg aactcgccac
     2161 tccctattac atcttcaggg atgagggcgt taaggtcgtg ctcgcgtctc caaagggcgg
     2221 aaagccgccc atcgacccca gaagcgagga acctcaggcg cagacggatt cgaccaagcg
     2281 tttcttcaag gacaaagagg cccagcaggc cttcgagaat accgcacagc tttcaacgat
     2341 aaacccaggc gattacggcg cggttttcta tccaggcggg cacggtccgc tttgg
//
LOCUS       DATMGX010000271         3579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_152102, whole genome shotgun sequence.
ACCESSION   DATMGX010000271 DATMGX010000000
VERSION     DATMGX010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152102"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="VNR65_04410"
     CDS             complement(<1..1595)
                     /locus_tag="VNR65_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWJ17954.1"
                     /translation="MCFSSKLFLVVGLLFVCQPALAEKRVALVVGNSNYKNAAALPNP
                     ANDAAAIAATLKGAGFDIVESRLDLQAAEMRRALRDFADQARDSDIAVVYYAGHGIEM
                     DGTNYLIPTDARLERDTDIYDEAFSLDRVLLAIEPARQLRLVIVDACRNNPFADTMKR
                     TVSSRSVSRGLARIEPTTTNTLVAFAAKAGLTALDGNSKNSPYATALVKYIAAPGLDL
                     RRAFGFVRDDVLQATGNRQEPYVYGSLGGADVALVPTKAVAPTPAANPQSEIRRDFEL
                     ALQLGNKDALSAFLTQYPDGYYASLAKLQLAKIAAEETRVAATEKARLAEQERMRLAT
                     EGAQRKDQEKAAADSKAAEESRIAAEKTKQAVQEQVAEAERQQVPADTSAADKSPESR
                     MAAADPTTSDAAAGPPAIERTQGPNLASLSAGPPQAELVKSVQTELRRVGCLTAAADG
                     EWNSASQRSMTLFNKNAGTKFDTKLASLDALDAIKLKPSRVCPLICGHGFKADGDECS
                     RIVCGAGSFLNDDNECEKRRGKTP"
     gene            2187..2975
                     /locus_tag="VNR65_04415"
     CDS             2187..2975
                     /locus_tag="VNR65_04415"
                     /inference="COORDINATES: protein motif:HMM:NF026241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M91 family zinc metallopeptidase"
                     /protein_id="HWJ17955.1"
                     /translation="MAPQSKQYLNNIWVLETSAAYRHEVVRYLDLVRTTSAGKTLFNY
                     INQRPQWMLIMPFHPTAKDPVNAYAYPQQGSEADSYAKFAPMMTSFTLPGNITIEIPT
                     GFGTGKGSMVYVDFHPATWRQLIKNTGRIAPGTGPGEILFHEMVHGYRMQCGLMRDDK
                     VDGHQGMDNIEEFYAILAANVYRSDRGFTSLRTDHWGFERLKQTDSYPEVFYDTFKKE
                     IDDWFDRQRPFCLDMARVPAKFNPFRHAAIARGFMTGPLTPMRL"
     gene            3104..>3579
                     /locus_tag="VNR65_04420"
     CDS             3104..>3579
                     /locus_tag="VNR65_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17956.1"
                     /translation="MSSSVLLKVYITPFADKGVGEAEKWSCDTAKEALNVVNAIWSKA
                     NIAFVINDCAIDKPLDIAKSARNDDRRLLDVLSLRHTPDNLVHIYLVNPIENLSAGGS
                     SYVDSDPEPASFIQWYGNVDANGRAWAHELGHLMSLNHVEIDYANERQAALRSNLMT"
BASE COUNT          718 a         1045 c         1035 g          781 t
ORIGIN      
        1 ggcgtcttcc cccgccgctt ctcgcattca ttgtcgtcat tgaggaacga gcccgcgccg
       61 cagacgattc tgctgcattc gtcgccgtcc gccttgaagc cgtgtccgca gatcagcggg
      121 cagacccggg aaggcttgag cttgatcgca tcgagcgcgt cgaggctcgc cagcttcgta
      181 tcaaattttg ttccggcgtt cttgttgaat agcgtcatcg agcgttgcga cgcgctgttc
      241 cattcgccgt ccgcggcggc ggtcaggcag ccgacacgac gcagctcggt ctgtaccgac
      301 tttacgagct cggcctgcgg cggtccggcg ctcagcgacg caaggttcgg cccttgcgtc
      361 ctctcaatcg ccggcggtcc ggcggcagcg tcgctggtgg tgggatcggc tgcggccatc
      421 ctgctttccg gagatttgtc ggctgccgat gtgtcggctg ggacctgctg ccgttcggcc
      481 tcggcgacct gctcctgcac cgcctgcttg gtcttttcgg cggcgatgcg gctttcctcg
      541 gcggccttcg aatctgcggc agccttctcc tggtctttcc tttgtgcgcc ctcggtggcg
      601 agccgcattc gctcctgttc agccagccgg gctttctcgg tcgccgcgac gcgggtctcc
      661 tccgccgcaa ttttggcaag ctgaagcttg gccaggctcg cgtaatatcc gtccggatac
      721 tgggtaagga aggcgctcaa tgcgtccttg ttgccaagct gcaacgccaa ctcgaaatcc
      781 cggcggatct ccgactgggg gttggcggcc ggtgtcggtg caacggcctt ggtaggcacc
      841 agcgccacat ccgcgccgcc gagcgagcca tagacatacg gttcctggcg gttaccggtg
      901 gcttgcagga catcgtcgcg aacaaatccg aatgccctgc gcagatccag gcccggcgcc
      961 gcaatatact ttaccagggc tgttgcatag ggactgttct tgctgttgcc atcgagcgcc
     1021 gtcaaaccag ccttggctgc gaaggcgacc agcgtattgg tcgttgtcgg ctcgatcctg
     1081 gcgaggccac ggctgacgct ccgcgagctg actgttcgtt tcatcgtatc cgcaaaggga
     1141 ttgttgcggc acgcgtcgac gatcacaagc cgcagctgtc tcgcgggctc gatagccagc
     1201 aaaacgcggt cgagcgaaaa cgcctcgtca tagatgtctg tatcgcgctc gagcctggcg
     1261 tccgtcggga tcaggtaatt cgtaccgtcc atctcaatgc cgtggcccgc gtagtaaacc
     1321 accgctatgt ccgagtcgcg ggcttgatcg gcgaaatctc gcagcgcccg ccgcatttcc
     1381 gccgcctgaa gatcgagcct tgattcgacg atatcgaaac ccgcgccttt caaggtagca
     1441 gcaatggctg cggcgtcatt ggccgggttt ggaagcgccg ctgcgttctt gtaattggaa
     1501 ttgccgacaa ccagcgctac gcgcttctcg gctagagccg gttgacaaac gaacaggagc
     1561 ccaacaacga gaaatagctt ggaagaaaag cacatggcga aacgtccacg aatatccgcc
     1621 tcagcatgcc aaataccttg ggcggatggc ggtgagataa gaatgtcctg ttcacatctt
     1681 actgtcgatg tggtcgacct cagttcatta gttctattcc aactctcgaa cgttctatca
     1741 cgaatgcgtt acaataacta ccgagagcga tccggacatc gcttaaatgg gagcaagtga
     1801 cgggcccttt tgtgtagcag ggggcaacgc gagcgctcgc gatacttaga gccatttctg
     1861 gcctccaggc gacatcatag cagttccggc ggatgtgggc cgcctgtcgc ggcttgttca
     1921 gcaagcgcag tttggcaatc gaaggatggg tgtcgcgcgt cctgggttga ctgccggccg
     1981 atacgacgcc tctacgagga tggccctgaa tacaatttat ggattagcgc actctgataa
     2041 cggcaccgcg ggcctgcgga ccgatggctg agttcttgaa ccgaaatcga gttcgccacg
     2101 acagatgtgc gggaaactag gtgtcgcgtt cctttgacgg ggcgctgctc cgcgccgagc
     2161 atcatcttga gccgaggaaa cccccaatgg cccctcaatc gaagcagtat ctgaacaata
     2221 tctgggttct ggagacgtcg gccgcctatc ggcatgaagt cgtacgttac cttgacctgg
     2281 tccgcaccac gagtgccggc aagacattgt tcaactacat caaccagcgt ccgcaatgga
     2341 tgttgattat gccctttcat ccgaccgcca aggatccggt gaacgcttat gcttacccgc
     2401 agcagggaag cgaagcggat tcctatgcga aattcgcccc aatgatgacg tcgttcacgt
     2461 tgccgggcaa catcaccatt gaaatcccca ccggcttcgg aactggcaag ggaagcatgg
     2521 tctatgtgga ctttcatccg gcgacatggc gtcagctaat caagaatacc ggccgcatcg
     2581 cgccgggaac tggacccgga gaaatcctgt ttcacgagat ggtgcatggc tatcgcatgc
     2641 aatgtggtct catgcgcgac gacaaggtcg acgggcacca ggggatggac aacatcgagg
     2701 agttctatgc cattctggct gcgaatgtgt atcgctcgga ccggggcttc acctcgcttc
     2761 gcaccgatca ttggggattc gagcgactga aacaaaccga ctcctatccc gaggtgttct
     2821 acgacacctt caagaaggag atcgacgact ggtttgatag acagcgcccc ttctgtctag
     2881 acatggcgcg cgtgccggca aagttcaatc cctttcgcca cgccgcgatt gcgcgaggtt
     2941 ttatgacggg tccgttgacg ccgatgcggc tctagtcagt ttggcatctg catatcgctt
     3001 ggacaaacat acagtttagc tgctcggcgc aaccattcac tgcgccgatt tcgcagaggc
     3061 acatcgaaga tatcgtcgaa atctccaaac gtccgaggaa cttatgtctt cttctgtttt
     3121 attgaaagtt tacatcacgc catttgctga taagggcgtc ggagaggcgg agaaatggag
     3181 ctgcgacacg gccaaagagg ccctcaatgt ggtcaatgcg atctggtcaa aggcgaatat
     3241 tgcgtttgtg atcaatgatt gcgccataga caagccgctc gatatagcaa agagcgcgcg
     3301 caacgatgat cggcgcttgc tggatgtcct tagcttgcga catacaccgg acaacctggt
     3361 ccacatttac ctcgtcaatc cgattgaaaa tctctccgct ggcggaagct catacgtcga
     3421 tagcgatccc gaaccggcca gtttcatcca gtggtatggc aatgtcgacg ccaatggtcg
     3481 ggcctgggcc catgaactcg gccatctgat gagtctgaat catgtcgaga tagactacgc
     3541 caacgagagg caggcggcgc tgcgcagtaa cctcatgac
//
LOCUS       DATMGX010000272         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_5572,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000272 DATMGX010000000
VERSION     DATMGX010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5572"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VNR65_04425"
     CDS             complement(<1..683)
                     /locus_tag="VNR65_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ17957.1"
                     /translation="MTPREIVRELDKYVVGQNQAKRAVAIALRNRMRRQKLSAELAEE
                     VIPKNIIMIGPTGVGKTEIARRLAKLANSPFMKVEASKFTEVGYVGRDVESMIRDLVE
                     IAIDMVREEKLEDVADKAELNAEERLLDLLLPAMPAPVQTRPAAQDPQQIGFNIGIGG
                     EPLLAGEPGSRTREKFRQQLREGKLDERIVELDVREKSMPAFEIITNQGVEEMDVNMK
                     DMLPNIFGQR"
     gene            complement(892..1089)
                     /locus_tag="VNR65_04430"
     CDS             complement(892..1089)
                     /locus_tag="VNR65_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17958.1"
                     /translation="MTKPPPNVLELPLEQRAVMAFKAAVEKLIIDHARTGAPFYIWRD
                     GKVIAMPKEELAAEAARLQAE"
     gene            complement(1086..1661)
                     /locus_tag="VNR65_04435"
     CDS             complement(1086..1661)
                     /locus_tag="VNR65_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HWJ17959.1"
                     /translation="MSPNLYVIAGPNGAGKTTFAREFLPNYGDCKNFVNADLIAEGVS
                     PFSPEAAAFRAGRLMLEEIDVHARRGEDFGFETTLSGRSHVGLIRDLKQRGYKVHLFF
                     LWLSAVDLALTRIKNRILEGGHSVPESVVRRRFDRSIRGFFVHHQPLADTWLLFDNSG
                     ATPQPIAIKEQHLRIINTALYNLLTARYGVP"
     gene            complement(1683..>2171)
                     /locus_tag="VNR65_04440"
     CDS             complement(1683..>2171)
                     /locus_tag="VNR65_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWJ17960.1"
                     /translation="DRIVGRILGMGDILSLIEKAEAHLDKKKSEEFAQKALSADGFSL
                     EDFRDQLRQVKKLGSLQSIMGMMPKIGPFANMQKSADKVDEKQLNRVEAIINSMTPHE
                     RDHHEVINGSRRKRIARGSGTSVQEINQLLRQYAQMRKMFKSMGKPSFARRLAGMKMP
                     GM"
BASE COUNT          409 a          644 c          617 g          501 t
ORIGIN      
        1 cgctggccga agatgttggg caacatgtct ttcatgttga cgtccatctc ctcgaccccc
       61 tggttggtga tgatctcgaa cgcaggcatc gatttttcgc ggacatccag ttcgacgatg
      121 cgttcgtcga gcttgccttc gcgcaactgc tggcgaaact tctcgcgagt gcgcgagcca
      181 ggttcgccgg ccagcaaggg ttcaccgcca attccaatgt tgaatccgat ctgttgcggg
      241 tcttgcgcgg cggggcgcgt ctgcaccgga gcaggcatcg ccggcagcag caggtcgagc
      301 aggcgctctt ccgcgtttag ctcggccttg tcggccacgt cttccagttt ctcttcgcgc
      361 accatgtcga tagcgatctc caccagatcg cggatcatgg actccacgtc gcggcccacg
      421 tagccgacct cggtgaactt cgacgcctcc accttcatga agggagagtt ggcgagcttc
      481 gccaagcggc gcgcgatctc ggttttgccc acgcccgtgg gtccgatcat gatgatgttc
      541 ttcgggatca cctcttcggc gagctcagcc gagagcttct ggcggcgcat gcgattgcgc
      601 agggcgatgg ccacggcgcg cttcgcctga ttctgcccta cgacgtactt gtcgagttcg
      661 cgtacgattt cgcgcggggt caattcgtcg agtgcgacgt gttcgtcatc tgcggttgct
      721 ggaagataaa tagccaatga atttccctct tgcggccctg aaggccgatt actttacaaa
      781 gcgagtttac gttgggccaa actaagcgac gctccttcgg agtgcggtgc gcctggaaaa
      841 ccgtgccatc ttgcgcatcc cgcgcccgcc agggagaccg ctctcccggc gttattccgc
      901 ttgcagacgt gcggcttccg ctgccagctc ttctttgggc atggcaatca ctttgccgtc
      961 gcgccaaatg taaaacggcg ctccggtgcg cgcatggtca attatcagct tctcgactgc
     1021 ggccttgaag gccatcacag cgcgctgttc caggggcagc tcaaggacat ttggaggcgg
     1081 cttggtcatg ggactccgta gcgagcggtc agtaggttat aaagcgccgt atttattata
     1141 cggagatgtt gttccttaat ggcgatcggt tgtggcgtgg cgccggaatt atcaaacaaa
     1201 agccatgtat cggctagcgg ctgatggtgg acaaaaaaac ctcgaattga acggtcaaac
     1261 cgacgtcgca caacagattc aggaacggag tgaccaccct ccaagatccg gttcttgatt
     1321 cgggtcagcg cgaggtcaac cgccgacagc cacagaaaga aaagatgcac cttatatccg
     1381 cgctgcttga gatcacgaat caggcccaca tgactgcgtc cagaaagcgt cgtctcgaat
     1441 ccaaaatctt cgccgcgtcg ggcatggaca tcgatctcct ccagcattaa acgccctgct
     1501 cgaaaagcag ccgcttcggg agaaaatggc gacacaccct ccgcaatcag gtcggcgttg
     1561 acgaagttct tgcagtctcc gtagttcgga agaaattcgc gcgcgaaggt cgtcttacca
     1621 gcaccgttgg gcccggcaat gacgtaaagg ttagggctca ttatttaggt gacgggctgc
     1681 ctttacatcc ccggcatctt catccctgcc agccggcgag cgaagctggg cttgcccatg
     1741 ctcttgaaca tcttacgcat ctgcgcgtac tggcgcagca actggtttat ttcctgaacg
     1801 ctggtccccg agccgcgggc gatgcgcttg cgccggctgc cgttgattac ctcgtggtgg
     1861 tcgcgctcgt gcggagtcat ggagtttatg atggcctcga cgcggttgag ttgcttttcg
     1921 tccactttgt ccgccgactt ctgcatgttg gcgaacggtc cgatcttggg catcatgccc
     1981 atgatgctct gtaacgagcc gagttttttc acctgccgca actgatcgcg aaaatcctcc
     2041 agcgagaagc cgtcggcgct gagcgccttc tgcgcaaact cttccgactt cttcttgtcg
     2101 aggtgagctt cggctttctc gatcagcgac agtatgtcgc ccatcccgag aatccgtccc
     2161 acgattcggt c
//
LOCUS       DATMGX010000273         2804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_93451, whole genome shotgun sequence.
ACCESSION   DATMGX010000273 DATMGX010000000
VERSION     DATMGX010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93451"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1964
                     /locus_tag="VNR65_04445"
     CDS             <1..1964
                     /locus_tag="VNR65_04445"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HWJ17961.1"
                     /translation="WQHEYPEDWLAFGNPWEFPRPEKAYTIGFGGSVESLHHSSVAQI
                     WRPSEVVEAVAYDTPIPGWRGKYVNSLRLWRSRAPDPLRLDVFNAGDYVAAQSEQVRA
                     ESISKVLYPSDSTPAGQELRLRQEYFFASASLQDLIHRHLKKHGDIMKLADKVAIQLN
                     DTHPAIAVAELMRLLVDVYGVGWEQAWTITQATFSYTNHTLLPEALESWPVPLMERLL
                     PRHMQIIYLINALHLDALREKGADQQLQSHVSLIDEYAGRRVRMGHLAFLGSHKINGV
                     SQLHTDLMKETVFHDLNTLYPDRIVNKTNGITFRRWLYEANPGLTKLLIETIGPDFLD
                     NADALRGIEKFADDSRFQEKYAAVRHANKEALAKIITDRLLIKVDPGALFDVQIKRIH
                     EYKRQLLNILETIAFYNEIHAHPTRDFVPRVKIFSGKAAASYHQAKLIIKLMHDVARV
                     VNNDPSVRGLLKVVFLPNFNVSLAQRIIPAADLSEQISTAGMEASGTGNMKFMLNGAL
                     TIGTLDGANVEICEHVGRDNIFIFGMTAQEVQDRRKNGIDAREVIAASPMLAEVLDSV
                     ASGVFSHDEKDRYRQLVDILTYHDHFMVTADFEAYCAAQREADMRWRDPKGWWRSATL
                     NTARVGWFSSDRTIKEYAEEIWRVPVRSA"
     gene            1975..>2804
                     /locus_tag="VNR65_04450"
     CDS             1975..>2804
                     /locus_tag="VNR65_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17962.1"
                     /translation="MTSASWRATDEDVASIIAATHHDPFAVLGLHDVPGGMVIRAFVP
                     SVARLQALVEPVDSVLDLERRGDSDFFEALVPGVTDRFAYRLRAEEKGERWEFLDPYA
                     FGQVLGPLDDYLLVEGTHQRLYERLGAHAITHEGVAGVLFAVWAPQAKRVSIVGDFNQ
                     WDGRRHTMRKRVDSGIWELFAPGLGEGTVYKYEIVGAGGNLVPLKADPFGFGSEMRPS
                     TASIVARTDNFTWDDEAHIAWRTNADARRSPMSIYEVHLGSWRRKWDNGFLTYDELAE
                     "
BASE COUNT          568 a          850 c          833 g          553 t
ORIGIN      
        1 gctggcagca cgaatatccg gaagattggc tggctttcgg caatccgtgg gaattcccac
       61 gccccgaaaa ggcctatacc ataggcttcg gcggctcggt ggaatcgctc caccacagct
      121 cggtggcgca gatctggcgt ccgtcggagg ttgtcgaggc cgttgcctat gacacgccga
      181 ttcccggctg gcgcggcaaa tacgtgaact cgctccggct gtggcgctcg cgtgcgcccg
      241 atcccttacg tctcgatgtc ttcaacgccg gggattacgt cgcggctcaa agcgagcagg
      301 ttcgagccga gtcgatctcc aaggtgctct atccgagcga ctcgaccccg gccggccagg
      361 agctcaggct gcgccaggaa tatttcttcg cctcggcctc gctgcaagac ctcatccacc
      421 gtcacctgaa aaagcacggc gacattatga agctcgccga caaggtggcg atccagctca
      481 acgacacaca ccccgccatc gcggtcgcgg agctgatgcg gctcctggtc gatgtgtacg
      541 gcgtcggctg ggagcaggcc tggaccatca cgcaggccac gttctcgtac acgaaccaca
      601 cgctgctgcc cgaggctctt gaatcctggc ctgtgccgct gatggagcgc ctgctcccgc
      661 gccacatgca gatcatctat ctcatcaatg cgctgcacct ggatgccttg cgggaaaagg
      721 gcgccgatca gcaactccaa tcacacgtct cgctcatcga cgaatatgcc gggcggcgcg
      781 tgcgcatggg gcatctcgcc ttcctcggct cgcacaagat caacggcgtg tcgcaacttc
      841 acaccgatct catgaaggag acggtgttcc acgacctcaa caccctctat cccgatcgca
      901 tcgtcaacaa gacgaacggc atcaccttcc ggcgctggct ttacgaagcc aaccccggcc
      961 taacgaagct tctcattgaa acgatcgggc cggatttcct tgataacgcg gacgccctcc
     1021 gggggattga gaaattcgct gacgactcga gattccagga aaagtacgcc gccgtccggc
     1081 atgcgaacaa ggaagcgctc gcgaagatca tcaccgacag gcttctcatc aaggtcgatc
     1141 cgggtgcgct cttcgacgtt cagatcaagc gcattcatga gtacaagcgc cagcttctca
     1201 acatccttga gacgattgcg ttctacaacg agattcacgc gcatccgacg cgggacttcg
     1261 ttccccgcgt gaagatcttc tcaggcaagg cggcggcgag ctaccatcag gccaagctca
     1321 tcatcaagct gatgcacgat gtggcgcgcg tggtgaacaa cgacccatcc gtgcgtggtc
     1381 ttttgaaagt ggtcttcctg cctaacttca atgtaagcct cgcgcagaga atcatcccgg
     1441 cggcggacct gtccgagcag atctcgaccg caggcatgga agcgtcgggc acgggcaata
     1501 tgaaattcat gctcaacggc gcgctcacca tcggaacgct cgacggggcg aacgtggaaa
     1561 tctgcgagca cgtcggacgc gacaacatct tcatcttcgg aatgacggcg caggaggtcc
     1621 aggaccgccg caaaaatggc atcgatgcac gcgaggttat cgccgcgtcg ccgatgctgg
     1681 cggaggtgct cgattctgtc gcatccggcg tattttcgca cgatgagaag gatcgttatc
     1741 gacagttggt cgatatcctg acataccatg atcacttcat ggttacggca gactttgaag
     1801 cctattgcgc ggcgcaaaga gaggctgaca tgcgctggcg tgacccgaag ggatggtggc
     1861 gctctgccac gttgaatacc gctcgcgtag ggtggttttc ttccgaccga acaattaaag
     1921 aatatgctga ggagatctgg cgcgtgcctg tgcgctcggc atgagggata aaaattgacc
     1981 agtgcaagct ggcgtgcaac cgatgaggac gtggcgagca tcatcgccgc gactcatcat
     2041 gatccatttg ccgtccttgg gctccatgac gtgcccggcg ggatggtgat ccgcgctttc
     2101 gttcccagcg tcgcgcgctt gcaggccctg gttgagcccg ttgactctgt cctggacctc
     2161 gaacggcgcg gcgatagcga tttcttcgag gccttggttc ccggtgtaac ggaccgcttc
     2221 gcctaccgcc ttcgcgccga ggaaaaaggc gagaggtggg aatttctcga tccctatgct
     2281 ttcggccagg ttctcggtcc tctcgatgat tacctgcttg tcgaagggac gcaccagcgg
     2341 ctttatgaac gcctgggcgc gcatgcgata acccacgaag gcgtcgctgg cgtcttattt
     2401 gccgtttggg cgccgcaagc caagcgcgtc tccatcgtgg gcgacttcaa tcaatgggac
     2461 gggcgccgtc acaccatgcg caagcgggtg gacagtggca tttgggagct tttcgcgccg
     2521 ggccttggag aaggaaccgt ctacaaatac gagatcgtgg gcgcgggggg gaatctcgta
     2581 ccactcaagg cggatccttt cggttttggc agcgagatgc ggccatcgac ggcttcgatc
     2641 gtggcgcgga cggacaactt cacatgggac gacgaagccc acatcgcctg gcggaccaac
     2701 gccgatgccc ggcgcagccc catgtcgatt tacgaagtgc atcttggctc gtggcggcgc
     2761 aagtgggata acggcttcct gacctacgac gagctggcgg agca
//
LOCUS       DATMGX010000274         3557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_298476, whole genome shotgun sequence.
ACCESSION   DATMGX010000274 DATMGX010000000
VERSION     DATMGX010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298476"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VNR65_04455"
     CDS             complement(<1..1255)
                     /locus_tag="VNR65_04455"
                     /EC_number="1.3.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:protochlorophyllide reductase
                     (ATP-dependent) subunit N"
                     /protein_id="HWJ17963.1"
                     /translation="MSADGAVPAVEVSERPVLRERGQREVFCGLTGIVWLHRKIQDAF
                     FLVVGSRTCAHLIQSAAGVMIFAEPRFATAIIDERDLAGLADLNEELDRVVTRLLERR
                     PDIKMLFLVGSCPSEVIKLDLHRAAERLSHDFAGKVRILNYSGSGIETTFTQGEDACL
                     ASLVPELPAEPRDALPSLMVVGALADVVEDQFARLFKELGLERVRFFPPRNSSDLPPI
                     GENTFFLLAQPFLSDTARGLQERGAKHIPAHFPLGEEGTTLWLQAAAKHFRVDKARFD
                     SVVEAPRERAKKALTAHKDKLEGKSIFFFPDSQLEVPLARFVSRELDMKLTEVGTPYL
                     HRQHLASELDLLQKDIVLSEGQDVERQLDRCRKTRPDLTVCGLGLANPLEAEGMTTKW
                     SIELLFTPIQGYDQAGDLAELFARPL"
     gene            complement(1252..1770)
                     /gene="bchF"
                     /locus_tag="VNR65_04460"
     CDS             complement(1252..1770)
                     /gene="bchF"
                     /locus_tag="VNR65_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-vinyl bacteriochlorophyllide hydratase"
                     /protein_id="HWJ17964.1"
                     /translation="MEPKQGRKLRRPLYSPEERIRRDSSPWTLVQGVLAPLQFAVFLV
                     SLVLVVRYLLTGSGAYSATVSIVVKTFVLYTIMVTGAIWEKKVFGQYLFAPAFFWEDV
                     MSFLVIALHTLYMAALIYGFLDTKQQMYVALAAYTAYVINAAQFLLKLRAARLDEARN
                     REALKLEPEMAR"
     gene            2056..2877
                     /locus_tag="VNR65_04465"
     CDS             2056..2877
                     /locus_tag="VNR65_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HWJ17965.1"
                     /translation="MPQPGSVNSPEEQSSAFIRRLVKTIEGEIIPRLLLSDKANSLPD
                     RRLSSGDVLTSDDIQEFGGLILALDQTRAYTFVEQKRAEAQSLEVIILDLLAPTASYL
                     GELWKADRCSFTEVTVGLSRLRQLLRDLSPDFESEAPNARHGRRALLLATPGDQHTFG
                     LFVVEAFFRREGWDVSGGQIDSADEIAHLVDRQWFAIAGFSLSSERFIDRLASLIRVV
                     RRQSCNRDIGVLVGGPLFVEQPELAERVGADAFAADGRMAVISARALLSRKAAPS"
     gene            2907..>3557
                     /locus_tag="VNR65_04470"
     CDS             2907..>3557
                     /locus_tag="VNR65_04470"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWJ17966.1"
                     /translation="MEILVSRIVNPFHSPKKTLGTLNADVVARLISSAADVALVLDKS
                     GVIRDVAIGSEDLSKAGIGGWIGVPWKDTVTSDSRHKVEELMREAALKLPTRWREVNH
                     AAPGGGHIPLRYSALQVRDDGRVVAFGRDLRALASLQQQLVDAQMSIERDYARLRQTE
                     LRYRLLFQIASESIIIADATTARITEVNPAALALIGEPSKKIAAQTLLELIDPADGP"
BASE COUNT          758 a          998 c         1068 g          733 t
ORIGIN      
        1 tcagcgggcg ggcgaaaagt tcggcaaggt cgcccgcctg gtcgtagccc tggatggggg
       61 taaacaaaag ctcgatcgac cacttcgtgg tcatgccctc ggcttcgagc gggttcgcaa
      121 ggccaaggcc gcagactgtc aggtcaggcc gtgttttccg acatcggtcg agctggcgtt
      181 ccacatcttg tccctcgctc aagacgatgt ccttctgaag caaatcgagt tccgaagcaa
      241 ggtgctggcg atggagataa ggcgtcccca cctccgtcag cttcatgtcg agttcgcggc
      301 tgacgaagcg ggccagtggc acttcgagct gcgagtccgg gaagaaaaag atgcttttgc
      361 cttcgagctt gtctttatga gcggtcagcg ctttcttcgc ccgttcgcga ggcgcctcga
      421 caaccgagtc gaagcgcgcc ttatcgacgc ggaaatgctt cgccgctgcc tgaagccaca
      481 gcgtcgttcc ttcctcgcca agcggaaagt gcgccgggat atgcttggcg ccgcgctctt
      541 gcagaccacg tgccgtgtcg ctcagaaagg gttgggcgag cagaaaaaac gtattctcgc
      601 cgatgggcgg aaggtctgaa ctgttcctcg gcgggaagaa gcgcacgcgc tccaggccaa
      661 gttccttgaa gagccgtgcg aactggtcct cgaccacgtc cgccagcgcg ccgacaacca
      721 taagcgaagg aagcgcatcg cgaggttctg ccggcaactc gggaacgaga gaagcaaggc
      781 acgcatcctc accctgggtg aaggtggttt cgatgccgct gcccgagtag ttgagaattc
      841 gaactttccc agcaaaatca tgcgacaggc gttctgccgc gcgatgcaga tcaagcttaa
      901 tgacttcgga ggggcaggac cctacaagga aaagcatctt gatgtcgggg cggcgctcca
      961 aaagccgtgt caccacgcgg tcaagctctt cattaaggtc tgcaagcccc gcaagatcgc
     1021 gctcatcgat gatggcggtc gcaaaacgcg gttccgcgaa tatcatgact ccagccgcag
     1081 actggatgag atgtgcgcaa gttctggaac cgacgaccag gaagaacgcg tcctgtatct
     1141 tgcgatgcag ccagacgatg cccgtcaagc cgcaaaacac ctcgcgctgg ccacgttcgc
     1201 gcaaaaccgg gcgctcgctg acctctacgg caggcacggc tccatcggcg ctcatcgggc
     1261 catctccggc tcaagcttga gcgcctcgcg attgcgcgct tcatcgagac gcgcggcgcg
     1321 aagcttcagc aggaactgtg ctgcattgat gacataggcc gtgtaagccg cgagcgcgac
     1381 atacatttgc tgcttggtgt caaggaatcc gtagatgagg gccgccatat agagagtgtg
     1441 aagcgcgatg acgaggaagc tcatcacgtc ttcccagaag aaggcgggcg caaaaagata
     1501 ctggccgaaa accttctttt cccagatggc gccggtgacc atgattgtat aaaggacaaa
     1561 cgtcttgacg acgatcgaga cagtagctga gtatgcacca ctgcctgtca acagatagcg
     1621 aacaacaagc accaagctga cgaggaagac agcaaattgc agcggcgcga gcacgccctg
     1681 cacgagtgtc cagggagagc tgtcgcggcg gatgcgttct tcgggcgaat aaagcgggcg
     1741 tctcaatttt ctgccctgct ttggttccat gcgcgctccc cacatcgtga cccccgtatt
     1801 tagaaccgac ggggcaggac tgtcaacata acttgacgta aaccccaatt tacaattact
     1861 ttgcagaaag actttccagg agcgcacaag aagtaaagtg gcgccggctt gtaaaatatt
     1921 tttgatgggg ctcccccttt cgctaagcct cggtctgggt cagcgccggg atcgtgttgt
     1981 gtcgcctctg gaggaaccta acgtatgtcg ccatcacggg acgcagttgg cgtaaaacaa
     2041 tgtcttaagg aagacatgcc gcaaccagga tcggtgaaca gtcccgagga gcaaagctca
     2101 gcgtttatca ggcggctggt caaaacgata gagggtgaaa ttattccgcg gcttctactt
     2161 tctgataagg ccaatagtct tcctgatcgc agattgtctt cgggcgatgt tctgacatcc
     2221 gatgacattc aagaattcgg cggtctgatc ctcgctctcg accaaacacg cgcctatact
     2281 ttcgtggagc aaaaacgcgc ggaggcgcaa tctcttgagg tcatcattct ggatctcctg
     2341 gcaccaacgg cctcctattt gggtgagctt tggaaagcgg atcgctgcag tttcaccgaa
     2401 gtgaccgtcg gtctttcccg gctacgccag ctgttgcgcg acctgagccc tgacttcgag
     2461 agcgaggctc ctaacgcccg gcatgggcgg cgggcgctgc tccttgcaac tccaggagac
     2521 cagcatacct ttggtttgtt cgtggtcgaa gcttttttcc ggcgggaggg ttgggacgtc
     2581 tccggaggcc agattgactc ggcggatgaa attgcccact tagttgacag gcagtggttt
     2641 gccatcgccg gtttctctct gagctcggag cgtttcatcg atcggctagc gtctctgatc
     2701 cgggttgtcc gtcggcaatc atgcaatcgc gatattggcg ttctggttgg cggcccgtta
     2761 tttgtagagc agccggaact ggcggagcga gttggagcgg atgcctttgc cgccgatggg
     2821 cgcatggccg ttatcagcgc ccgcgcattg ctcagtcgaa aagctgcccc gagctgaacc
     2881 ggagcacaaa ggacttaagc tcaaaaatgg agattctggt ctctcgcatt gtgaacccct
     2941 ttcattcacc aaagaaaacg ctcggcacgt tgaacgctga cgtcgttgcg aggctgatct
     3001 cgtcggcggc ggatgtggcg cttgtgttgg acaagagcgg cgtcatcagg gacgtggcga
     3061 ttggcagcga ggatctctca aaagccggga ttgggggatg gattggcgtt ccgtggaagg
     3121 ataccgtcac ctccgacagc cgtcataagg tcgaggaatt gatgcgcgag gctgccctca
     3181 agctgccgac gcgctggcgg gaagtcaatc acgccgcccc agggggtgga catattccgc
     3241 tccggtactc cgcccttcag gtgagagatg atggcagggt tgtcgctttc ggtcgcgatc
     3301 tcagagcgct ggcgagtttg cagcagcagc ttgtcgacgc tcagatgtcg atcgagcggg
     3361 actatgcgag gttgcgccag acggagctgc gctaccgtct tctgtttcag atcgcctcag
     3421 agtccatcat catcgcggat gcgaccacgg cgcgcatcac cgaggtcaat ccggccgcgc
     3481 ttgccctgat tggtgagccg tcgaagaaaa ttgcggccca aaccctcctt gaactgatcg
     3541 atccggccga cggaccg
//
LOCUS       DATMGX010000275         3754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_349691, whole genome shotgun sequence.
ACCESSION   DATMGX010000275 DATMGX010000000
VERSION     DATMGX010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349691"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..3198)
                     /locus_tag="VNR65_04475"
     CDS             complement(103..3198)
                     /locus_tag="VNR65_04475"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17967.1"
                     /translation="MMRNSPVISFLLLAILSFAPLLQSQQIDTGLLSEMHWRNVGPFR
                     GGRTRAVSGVPGQPNVFYIGAVNGGVWKTADFGRTWAPIFDREATGSIGALEVAPSDP
                     NVIYVASGEGLHRPDLSVGDGIYKSTDGGATWTHLGLRESQQISQMAIDPHDPNRLFV
                     AVAGHPYGPNPERGIYRSTDGGQTFDKVLYKDENIGAGDVKIDPSDPSIVYATLWEAR
                     EGPWENGEFSGTNGGVFKSTDGGKNWHPLAGGLPNGIVQAYVAIARSNPKRLFASVAT
                     KDKVELYRSADGGETWTIATTGARPGSRIGGGDLPVPMIDPKNPDVVYMIGTVTWKSA
                     DGGKTWTGFRGAPGGDDYQNGWINPNDSNTIILTSDQGAVVTVNGGASWSSWYNQPTA
                     QMYHVNADNAFPYRLCSGQQESGSVCISSRGNDGEITFRDWHPVAAEEYGYVVPDPLD
                     PDIVFGGKLTRYDRRTAQGQNVMPVAIPGPDFRVIRTEPIVFSPIDPHLLYFASNTLW
                     TTRNGGQSWKQISPDLTRKTWEVPATLGIFRTEPSARPKQRGVIYAVAPSPLDINRIW
                     AGTDDGLIWLTTDGGAHWNNITPPQLSAWQKVSVLEASHFDPETAYAGINTLRLDDLR
                     PHIYRTRDSGKTWTEITSGIPENENVNSVREDSKRKGLLFAGTERAVYVSFDDGDRWQ
                     SLRLNMPATSVRDLTIKDNDLAVGTHGRGFWILDDITPLRQLDDKVVASNAFLFTPEL
                     AYRVRWDANTDTPLPPDEPAGQNPPDGAIFNYYLKSAAVGPVMLEILDSAGTVVRRYS
                     SADPVPPTDPELPIPAYWLRPPQSLSSAAGLHRFVWDMHYVPVPGMKPEYPIAAVPWN
                     TVPAPTSPWVMPGKYGVVLTIDGQRYTQPLTIAMDPRVKTSIADLQKQFELSKQIYDD
                     LLALQPVVDQAASALTKLKSVRTKASGPEAAKIDAAVQELESLEGAEGRRRRGAQTDT
                     LSGVHTSLMEMLSVLQDVDAAPTTQAAQAVPKLNQSTTSLVERWQEIASKQLAPLKAQ
                     P"
     gene            complement(3536..>3754)
                     /locus_tag="VNR65_04480"
     CDS             complement(3536..>3754)
                     /locus_tag="VNR65_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17968.1"
                     /translation="AGIGCLPDTAADGAEIKRSSITRYTGDGNHTAAAKWANHSPLQA
                     AKEIGRNRLGAGGKVQGKNNQKKEQQLI"
BASE COUNT          720 a         1106 c         1125 g          803 t
ORIGIN      
        1 aatgcggcct actggtatcg tcgagccgga caaccccccg cgcagagctc gattgagcac
       61 gagtggcgcg aaatcacaaa ggcgctgctg gcggagtagc tttcagggct gcgctttcag
      121 cggcgccagt tgtttgctcg cgatctcctg ccagcgctcc accagggatg tcgttgactg
      181 gttcaactta gggacggctt gtgctgcttg cgtcgtaggc gcagcatcca cgtcctgtaa
      241 cacggacagc atttccataa gcgatgtgtg aacacccgaa agggtgtcgg tttgcgctcc
      301 gcgtctgcgg cgaccctctg ctccttccag cgactccagt tcctgaacgg cggcgtcaat
      361 cttggcagcc tccggtccgc tcgccttggt gcgcacggac ttcaactttg tcagcgccga
      421 cgcggcctgg tccaccaccg gctgcagcgc tagcaaatcg tcatagatct gctttgacaa
      481 ctcgaactgc ttttgtaaat cggcaatcga ggtcttgacg cgagggtcca ttgcgatcgt
      541 cagcggctgc gtgtaacgct gtccgtcgat ggtgagaacg acgccgtatt tgcccggcat
      601 gacccatggc gaagtcggtg cgggaaccgt gttccaggga accgcggcaa tcggatattc
      661 aggcttcatc cctggcaccg gcacgtagtg catgtcccag acaaaacggt gcagtcccgc
      721 cgcgctggac agcgattgtg gcggacgcaa ccaatacgcg ggaatcggca gctcagggtc
      781 agttggggga actgggtcgg cactggagta tcgccgcacc acggtgcccg cactatcgag
      841 gatctcaagc ataaccgggc cgacagccgc tgactttaag taatagttga agattgcccc
      901 atccggggga ttctgtcccg cgggctcgtc cggcggcaga ggcgtgtcgg tattggcatc
      961 ccaacgaacg cgataggcaa gctcgggcgt gaacaggaag gcgttgctgg cgacaacttt
     1021 gtcgtccaac tgacgcaatg gcgtgatgtc gtcaaggatc cagaagccgc ggccatgcgt
     1081 ccccaccgcg aggtcgttgt ctttgatcgt caggtcgcgg accgaggtcg cgggcatgtt
     1141 cagccgcagg gattgccagc ggtcgccgtc atcaaaagag acgtacaccg cgcgctcggt
     1201 acccgcaaac agcaggccct tgcgcttgga atcttcgcgc acggaattca cgttctcgtt
     1261 ttccggaatg ccactggtaa tctcggtcca cgtcttgccc gaatcgcggg ttcgatagat
     1321 gtgcggccgc agatcgtcga gacgcaaggt gttgatgcct gcatatgcgg tctccggatc
     1381 gaagtggctg gcttcgagga ccgaaacctt ctgccatgcc gacaattgtg gcggcgtgat
     1441 gttgttccag tgcgctccgc cgtctgtggt cagccagatc aagccatcat cggtacccgc
     1501 ccagatgcgg ttgatgtcga gcggcgacgg cgcgaccgcg tagatgaccc cacgctgctt
     1561 gggccgggcc gaaggctcgg tgcgaaagat gccgagggtt gcgggcactt cccaggtttt
     1621 gcgggtgagg tcaggactga tctgcttcca gctctgtccg ccgttgcgtg tagtccacaa
     1681 agtgttcgac gcgaagtaga gaagatgcgg atcaatcgga gaaaacacaa tcggttccgt
     1741 tcttatcacg cggaagtccg gccctggtat cgccaccggc atcacgttct ggccctgggc
     1801 agtgcgccgg tcgtaacgcg tcagcttgcc gccgaagaca atgtcgggat cgaggggatc
     1861 gggcactaca tagccgtact cttccgccgc cacgggatgc cagtcacgaa acgtaatctc
     1921 gccatcgttg cctcggctgg aaatgcaaac agagccgctc tcctgctggc cgctgcatag
     1981 gcgataggga aaagcgttgt cggcgttcac gtgatacatc tgcgccgtgg gctggttgta
     2041 ccaggaactc caactcgccc cgccgttcac ggtgacgacg gccccctggt cgctcgtcaa
     2101 aatgatcgtg ttggaatcgt tgggattgat ccagccgttt tgatagtcat cgccgccagg
     2161 agcgccgcga aagccagtcc aggtcttgcc gccatccgcg gatttccaag tgacggtgcc
     2221 gatcatgtaa accacgtcag ggttcttagg atctatcatc ggcacgggca aatcgccgcc
     2281 gccgatgcgg cttccaggac gcgcgccagt ggtcgcgatt gtccaggttt caccgccgtc
     2341 ggcggaacga tagagttcga ctttgtcctt ggtcgcaacg gaagcgaaga gccgcttggg
     2401 attgctgcgc gcgatggcaa cgtaggcttg cacgatgccg ttcggcagtc cgcctgccag
     2461 gggatgccag ttctttccac cgtcggttga cttgaacaca ccgccgttcg taccgctaaa
     2521 ttcgccgttc tcccagggcc cttcccgtgc ctcccacaat gtggcataaa cgatactcgg
     2581 atccgatggg tcgatcttca cgtcaccagc gccgatgttc tcgtctttgt agagcacctt
     2641 gtcgaatgtc tggccgccat cggtcgagcg atagatcccg cgttccgggt tggggccgta
     2701 agggtgtccc gcgacagcga cgaagagccg gttaggatcg tgggggtcaa tcgccatctg
     2761 cgaaatctgc tggctctcgc gcaatccaag atgggtccac gtcgctccgc catcggtaga
     2821 tttatagatc ccatcgccaa ccgacaggtc gggacgatgc agcccttcac cgctggcgac
     2881 gtagatgacg ttggggtcgg aaggcgccac ctctagggca ccgatcgagc cggttgcttc
     2941 gcgatcgaag ataggcgccc atgtccggcc gaaatcggcg gtcttccaca cgccaccatt
     3001 cactgcgccg atgtagaaga cattcggctg gccgggcaca cccgagactg cgcgggtacg
     3061 gcctccgcga aatggcccga cgtttcgcca gtgcatctcg ctcaaaagtc cggtgtcgat
     3121 ttgttggctc tggagcagag gagcgaaact gaggattgcg agcagaagga aagaaattac
     3181 cggcgaattt cgcatcatta aggagtcacc atgattcgtt gatcttcaga ctgcggaaaa
     3241 atcagcgatt gctgcgcggc acttgactgc cgctttcacg atttctgagt tgtcatcacg
     3301 aacggccttc aggccgtgag ggatctgctg ttcttccccg gccgtcgttc caggcacaaa
     3361 caagagcaga ttcctcgctt cgctcgtaat gacaattcca gacgcgaaag ccaattccaa
     3421 acacccaggc tcgaacgcgg ccttgaatct accgaggccc cgcgaaagtg taatctattt
     3481 gcacacatct ttgcaggggt agaggatctc cgatttgaaa cattgtgccc ggcgtttata
     3541 tcaattgctg ctctttcttc tgattgtttt tcccctgaac ctttccacca gcgcccaatc
     3601 ggtttctccc gatctctttg gcggcttgca atggagaatg attggcccat ttcgcggcgg
     3661 ccgtgtggtt gccgtcgccg gtgtaccggg tgatggagct acgttttatt tcggctccgt
     3721 cggcggcggt atctggaaga caaccgatgc cggc
//
LOCUS       DATMGX010000276         2110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_69648, whole genome shotgun sequence.
ACCESSION   DATMGX010000276 DATMGX010000000
VERSION     DATMGX010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69648"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2110
                     /locus_tag="VNR65_04485"
     CDS             <1..>2110
                     /locus_tag="VNR65_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ17969.1"
                     /translation="NEGKVYLPDQAERAIRNYFSPGNLTALRELALRRTAQRVDAQMV
                     DYMRAHRIEGSWPASERVLVLVDSSRGANAIVRHAKRMADRLRAQWTAIHIETPADAR
                     ATEAERDKIAQTLRLAQRLGAAAISIPGEDAALTVAEYAEANHFAHIITAKSVRPHWR
                     DYFSDSFTEKLIRAAGDASVHVVARAKDPQAQNATDYDNKAAKPSRTELKAYIVSLAY
                     VAAAILVAALLRHVLGVSNLTQVFLIAILASAVTYGLWASLFACLVSVLSYNFFFLPP
                     LYTFTIADPENVVVLFFFAVVALIASNLAARVRDQAVAARERMRITEELYLFSRKLAG
                     VASLDDLLWATVHQIALMLKVRVVVLLPEDGSLVVKAGFPPEDMLDEADLAAARWCFE
                     KNQATGRGADTLPGAKRRFMPLRTGRGAVGVAGIDSDKPGLLLTPEQSRLLDALSDQA
                     ALAIDRVNLAQDIDRARLAAETDRLRAALLTSISHDLRTPLASILGSASSLVTHRHEF
                     DPTVQASLMRTILEEAERLNRFIGNLLDMTRLESGPLKPHSGAADLSDIIGATLQRAD
                     KILENHSVEIDLESGLPLLDLDMVLMEHVLFNLFDNAAKYAPQGSRMTVTASHEHDSV
                     ILRVLDEGEGIPEADLERIFDKFFRVRRSDRQRAGTGLGLAICRGFVEAMGGSITAAN
                     RTDRTGAVFTIRLPVPVTSKL"
BASE COUNT          405 a          639 c          604 g          462 t
ORIGIN      
        1 tcaacgaagg caaggtctat cttccagacc aagcagagcg tgccatccgg aattattttt
       61 cgcccggtaa tcttacggcc ctacgcgagc tcgcgctgcg ccggacggcg caaagggtcg
      121 acgctcaaat ggttgattat atgcgtgctc atcgcattga agggtcgtgg ccggcgagcg
      181 agcgcgttct tgttttggtg gattcctcgc ggggagccaa tgccatcgtg cgccatgcca
      241 aacgtatggc ggatcgactg cgcgcgcagt ggactgcgat ccatatcgaa acgccggccg
      301 acgcgcgtgc aaccgaagcc gagcgcgata agatcgccca gacacttcgc ctcgcgcaaa
      361 ggttaggcgc tgcagcaatt tctatacccg gagaggatgc cgctttgacc gtcgccgaat
      421 atgcggaggc caatcatttt gctcatatca ttaccgccaa atccgtgcga ccgcattggc
      481 gggattattt tagtgactca tttacagaga aactgatccg cgccgcgggt gacgcgagcg
      541 ttcatgtcgt cgcgcgcgcg aaagatcccc aagcccaaaa cgccaccgat tatgacaaca
      601 aggcggcaaa gccaagtcgc acggagctta aggcctatat cgtgagcctc gcctatgtcg
      661 cggccgcgat cctcgttgcc gccctgctcc ggcatgtgct tggtgtctca aacctcacac
      721 aagttttcct tatcgcaatc ctagcgagcg ccgtgacata cggcctatgg gcctcgctat
      781 ttgcctgtct ggtcagcgtg ctttcctata attttttctt cctgccgccg ctctacacat
      841 tcacgatcgc agatccagag aatgtcgttg tcttattctt ttttgctgtg gtggctctga
      901 ttgcgagcaa tctcgccgcg cgggtccgtg accaggcagt cgcggcgcgc gagcgcatgc
      961 ggatcaccga ggaactctat ctttttagcc ggaagctcgc tggtgtcgcg tcgcttgacg
     1021 acctgctctg ggcgacggtg catcagatcg ccttgatgct gaaggttcgg gtcgtcgtac
     1081 ttttacccga agacggcagt cttgttgtca aggctgggtt tccacccgag gacatgctcg
     1141 acgaggcgga tctcgctgcg gcccgctggt gctttgagaa aaatcaagcg acagggcgcg
     1201 gcgccgatac gctgcccggt gccaagcgcc gcttcatgcc tttgcgcacc ggacgcggcg
     1261 ccgtgggcgt tgccgggatc gatagcgaca agccgggatt gctcctgaca ccggaacagt
     1321 cccggctttt ggacgccttg tcggatcaag cagctcttgc gatcgatcgc gtcaatcttg
     1381 cccaagacat tgaccgggct cgtctagcgg cggagaccga tcgcctgcgc gcggccttgc
     1441 tgacctcgat ttcgcatgat ctgcgcaccc cactcgcttc gattctcggc tcggcgagca
     1501 gccttgtcac gcatcgacat gagtttgatc cgactgttca agcaagcctc atgcgcacca
     1561 ttctggagga ggccgagcgg ctcaaccggt ttatcggcaa tcttctcgac atgacgaggc
     1621 tcgaatccgg accgctcaaa ccgcactcag gagccgccga tctctccgac atcatcggcg
     1681 cgaccttgca aagggcggat aagatcctgg aaaaccactc ggtcgaaatc gatctcgaaa
     1741 gcggtttgcc gctgctcgat ctcgacatgg tactgatgga gcacgtgttg ttcaatctct
     1801 tcgataatgc ggcaaaatac gcaccgcaag gttcccgcat gacggtcacg gccagtcatg
     1861 agcacgattc cgtcatcctc cgggtactcg acgagggcga gggcattccc gaggctgatc
     1921 tcgaacgtat cttcgacaag ttctttcggg ttcgccgttc cgaccggcag cgtgccggca
     1981 ccgggttagg ccttgcaatc tgccgcggat tcgtcgaagc catggggggc tcgattacag
     2041 ccgccaatcg gactgatcgg accggcgcgg tgttcaccat caggcttccc gttcctgtca
     2101 cgtcgaagct
//
LOCUS       DATMGX010000277         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_198925, whole genome shotgun sequence.
ACCESSION   DATMGX010000277 DATMGX010000000
VERSION     DATMGX010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198925"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VNR65_04490"
     CDS             <1..910
                     /locus_tag="VNR65_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HWJ17970.1"
                     /translation="SQLDDEEIRAIARSASDLGAVSQSVLESIADEFVIKVTIDGGLR
                     GSSEQVERMLEEIMPPQQVRQIMADVRARMNEAVWPRLVELPPHLLAQFLNKEHPQVI
                     TYVLSKASASLSAEVVALFPNHLRNEVMGRMLTNKLVTQQSLRLLEQILRDELLLKVA
                     KTTGQDTHERVAKIVNKLSKKEMEEVLDSIGATKPKAAETVRGLLFTFDDIRKLPPRT
                     RVTLFESLEADKLVPALNGADDEMKEFILSSVPSRTRRVIEQDLAAAPPMTDKEVEKA
                     RREIADFALQLADQGPISLAFAEAEE"
     gene            925..1866
                     /locus_tag="VNR65_04495"
     CDS             925..1866
                     /locus_tag="VNR65_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HWJ17971.1"
                     /translation="MIQESKKSEAVGITEKLKEIAKLSLDRLPVLNSIFEKMSVTCVE
                     RFRDYCSPAFTAFVNQVVSGDSWDLLEASADSVAVIFYCREWDARILIGLERRFIFAI
                     LEAMFGGDGSEQPFDATRPFTALEMRIGRLVCEFAAKALQASFGSVCEISLVYERTET
                     ALEFTTLGQNSMIMIEAKVLFQVLDQGGVMFVLIPQNSMNPIRQKLERERKPLPSSYD
                     PRWTTALTSRVSKAEVILNAVIEGKSIELDDIVRLEVGKTLELSGTDQDIILECEGDR
                     LFRGRLGQARGNFTITVDAPINEDLFLEVLEGTQIDS"
BASE COUNT          509 a          622 c          624 g          479 t
ORIGIN      
        1 ctcccagctc gacgacgaag agatccgcgc gatcgcgcgc tcagcctccg accttggcgc
       61 cgtctcacaa tccgtgcttg agagcatcgc ggacgaattc gtgatcaagg tgacgatcga
      121 cggcgggttg agagggtcct ccgaacaagt ggagcgaatg ctggaggaga tcatgccccc
      181 ccagcaggtc aggcagatca tggcggatgt ccgcgccagg atgaacgagg ccgtctggcc
      241 tcgcctggtc gaactgcctc ctcatcttct ggctcagttc ctgaacaagg agcatcccca
      301 ggtcatcacc tatgtcttgt caaaagcgtc tgcatcgctt tcagccgaag tcgtggcttt
      361 gtttccgaac cacttacgaa atgaagtgat ggggcggatg ctcaccaata aactcgtcac
      421 gcaacaatct ctgcgccttc tggagcagat cttgcgggac gagctcctgt tgaaagtcgc
      481 gaagacaacc ggtcaggaca ctcacgagcg tgtcgcgaaa atcgtgaaca agctcagcaa
      541 gaaagagatg gaggaggtgc tcgatagtat cggggcaacc aagccgaagg ctgcggaaac
      601 ggttcgaggg ctgctcttca cctttgatga catcagaaaa ctgccaccaa gaacaagggt
      661 cactcttttc gagagtctcg aggcggataa actggttcct gcgctcaacg gcgcggacga
      721 tgaaatgaaa gagttcatac tgtcgtccgt gccatcgcgg acgcggcggg tcatcgaaca
      781 agacttggcc gccgcaccgc ccatgactga taaggaggtc gaaaaggctc gccgggaaat
      841 tgccgatttt gccctccaac tcgccgatca gggccccatt agcctcgcat tcgcggaagc
      901 agaagaataa accccggagg actcgtgatt caggaatcga aaaagagcga ggccgtcggc
      961 attaccgaaa agctgaagga gatcgctaaa ctatcgctcg accggttacc tgttttaaac
     1021 tccatcttcg aaaagatgtc ggtcacatgc gtggagcgat tccgggatta ctgctcgcct
     1081 gctttcacgg ccttcgtcaa tcaggtcgtc tcaggagact cctgggacct tctggaggcg
     1141 agcgcggata gtgtcgccgt gatcttctac tgccgtgaat gggatgccag gattttgatc
     1201 gggctggagc gccgcttcat cttcgcaatc ctggaagcga tgttcggcgg cgatgggagt
     1261 gaacaaccct tcgatgcgac gcgccccttc acggctcttg agatgcgaat cgggcggctc
     1321 gtttgcgaat tcgccgcgaa agccctgcaa gcctctttcg gttcggtttg cgagatttct
     1381 ctcgtttacg agcgaaccga aacagcgctg gagttcacga cgctcggcca gaacagcatg
     1441 atcatgatcg aggcaaaggt cctttttcag gtcctggatc agggtggcgt catgtttgtg
     1501 ctcattcctc aaaacagcat gaaccccatc cgccagaaac tggagcgcga acgcaagccc
     1561 ctgccctcat cctacgatcc gcgatggacg actgccctga ccagccgcgt ctccaaggcg
     1621 gaggtcattt tgaatgctgt gattgaaggc aagagcatcg agctggatga cattgtgcgg
     1681 ctggaagtcg gcaagacgct cgagctaagc ggcaccgatc aagatatcat cctcgaatgc
     1741 gagggtgatc gcctctttcg cggccgtctc ggtcaggccc gcggcaactt tacgataacg
     1801 gtcgatgcac cgatcaacga agatttattt ctggaagttc ttgagggaac ccagatcgat
     1861 tcttagaatc gatccgcttt tgatggaatc aaaagcgggc tccaagtttt tgttttgtcg
     1921 tgcgactcta tcggaaaccg cttcgcactt ttccgtacgc actctaagca gaattttccg
     1981 gacgatccaa cggatcgcca ccgccgtcat cccggaaagg cgaagcctta tccgggatcg
     2041 catcatattg aagcccctac atttttacga tcccggctcg cgcttcgctt ggccgggatg
     2101 acgaagcgtg ccgcctactt ccgtttgaac acgacgtcgt agtaggctcc tgcttccggg
     2161 tgggaggcaa atttatcccg gcgggcaagc ttaaaattgt acccgcgcaa aaagcactcg
     2221 atgtccgaaa gttt
//
LOCUS       DATMGX010000278         2397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_43653, whole genome shotgun sequence.
ACCESSION   DATMGX010000278 DATMGX010000000
VERSION     DATMGX010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43653"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VNR65_04500"
     CDS             <1..280
                     /locus_tag="VNR65_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17972.1"
                     /translation="KPEVKAEPKAEAKTEPKPEAKAEPKAEVKAEPKPGVKAEPNPEA
                     KAEPKSEAKAEPKAEVKAEPKPEAKAEPTPEAKADSKPEEPKPETVSA"
     gene            283..1914
                     /locus_tag="VNR65_04505"
     CDS             283..1914
                     /locus_tag="VNR65_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternate F1F0 ATPase, F1 subunit alpha"
                     /protein_id="HWJ17973.1"
                     /translation="MEPESLQHDFDSAFAGIRQVREGFKPRLTPREVGIVTNVSTGIA
                     TLSGLPGAGYDELVRFRGDLLGIAFNVDEDEIGVILLGEYQDLHAGDEVERTGRVMDI
                     AVGDGLLGRVINPLGKPLDGKGPLAGDQRLPVERPAPPIMDRAPVVVPLQTGLKAVDA
                     LIPIGRGQRELILGDRQTGKTAIAIDTILNQRGQNVLCVYCAIGQRASAVAKVVANLR
                     EKGAMEYTVVVVAEGNDAPGLVYITPYAATSIAEYFMEKGRDVLIVYDDLTQHARAYR
                     ELSLLLRRPPGREAFPGDIFYIHSRMLERSTHLGKERGGGSLTALPIIETEAQDISAY
                     IPTNLISITDGQIYLSPTLFELGILPAVDVGKSVSRVGGAAQRAAYRAVAGSLKLAYS
                     QFEELETFAKFGTRLDESTRKIIDHGQRIRACLKQPESQPVSMIEQITVLLALTANLF
                     DAVPIEKVEGAEQALQKATADIPADVLGRLTSADKLSDDDRKAILEIDSHALAPFQPT
                     PKPKPASEPKPTAAPKSTSKPKPAAEPKPTAKASP"
     gene            1923..>2397
                     /locus_tag="VNR65_04510"
     CDS             1923..>2397
                     /locus_tag="VNR65_04510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HWJ17974.1"
                     /translation="MESLRRKIDGAGDLEGVVRSMKALAASSIGQYEKAVQSLDDYYR
                     TLELALVACLHQAESVRFAEAKRPPREGPHEGIIGALVFGSDQGLVGRFNEVVVEFAV
                     NKLKTLPGKTTKIWAVGDRAHALMVDSGMAPVKLLSVPTSVNAITPLIGQILIEIE"
BASE COUNT          553 a          718 c          711 g          415 t
ORIGIN      
        1 caagccggaa gtgaaagccg agccgaaggc agaggcaaaa acggagccga agcctgaagc
       61 aaaagccgag ccgaaggcag aggtgaaagc cgagcccaag ccgggagtga aagctgagcc
      121 aaacccggaa gcgaaggccg aaccgaaatc agaagcgaaa gccgagccga aggcagaagt
      181 gaaagccgag cccaagccgg aagcgaaagc ggagccgacg cctgaagcga aagcagactc
      241 caagcctgaa gaacctaagc ccgaaactgt gagcgcataa agatggaacc tgagagcctc
      301 cagcacgatt tcgacagcgc gtttgctgga ataagacaag tgcgcgaagg tttcaagccg
      361 cgactgacgc cgcgcgaagt gggcatcgtc acgaatgtct ccaccggaat cgccacgctt
      421 tcgggacttc ccggcgctgg ctatgacgaa ttagtgaggt tccggggcga tctgctgggc
      481 atcgcgttca acgtggatga ggacgaaatc ggcgtcatcc tgctcggcga atatcaggat
      541 ctgcacgcgg gcgacgaagt cgaacgcacc gggcgagtca tggacatcgc ggtcggagac
      601 ggtttgctcg ggcgggtgat caacccgctg gggaaaccgc tcgatggcaa agggccgctt
      661 gccggggacc agcgcctgcc ggtggagcgt ccggctcctc ccatcatgga tcgcgcgccc
      721 gtagtcgtgc ctttgcagac cggactcaag gccgtcgatg cgctcattcc tattgggcgc
      781 gggcagagag aattgattct cggcgatcgc cagacgggca agacggccat cgcgatcgac
      841 accatcttga accagcgcgg gcaaaatgtg ctctgcgtct attgtgcgat cggacagcgc
      901 gcctccgccg tcgccaaagt tgtggccaac cttcgggaaa aaggcgcgat ggagtacacc
      961 gtggtggttg tcgccgaagg caacgacgcg cccggtcttg tctatatcac gccctacgcc
     1021 gcgaccagca tcgctgaata tttcatggaa aagggacgcg acgtgctcat cgtttacgac
     1081 gatctcacgc agcacgcccg tgcctaccgc gagctatcgc tgctgcttcg ccgtccgccc
     1141 ggccgcgaag cgttccccgg cgacatcttc tacattcatt cgcggatgct ggaacgctcg
     1201 acgcatctcg gcaaggagcg cggcggcggt tctctcaccg cgcttcccat catcgagacc
     1261 gaggcgcagg atatctccgc ctacattccg accaatctca tctctatcac agacggtcaa
     1321 atctacctct cgcccacgtt gttcgaattg ggaatacttc cagcggtcga tgtcggcaag
     1381 tcggtttcgc gcgtgggcgg agcggcgcag cgagccgctt atcgcgccgt cgcgggcagc
     1441 cttaagcttg cctactctca gttcgaggaa ttggaaacat tcgccaagtt cggcacccgc
     1501 ctcgatgaat ccacccgcaa gatcatcgat catgggcagc gcattcgcgc ttgcctcaaa
     1561 cagccggaat cgcagccggt gtcgatgatc gagcagatca ccgtgctcct ggctttgaca
     1621 gcgaacctgt tcgacgcggt tccgatcgag aaggtggagg gtgcagagca ggcgctccag
     1681 aaagcaacgg cggatattcc ggcagacgta ctggggcgat taacgtcagc cgacaaattg
     1741 agcgatgacg accgaaaggc gattctagaa atcgactccc atgcgcttgc tccgttccag
     1801 ccaactccta aaccaaaacc tgcctctgaa ccaaaaccca ccgctgcgcc caaatccact
     1861 tctaaaccca aacctgccgc cgaacctaaa cccaccgcca aggcctcgcc atgagcggta
     1921 cgatggagag tctgcgtcga aaaatcgacg gcgcggggga tctcgaaggc gtcgttcgtt
     1981 cgatgaaggc gctggcggca tccagcattg gacaatacga aaaggcagtc caatcgctgg
     2041 atgactacta tcgcacgttg gagctcgcat tggttgcgtg tctccatcag gcggagtcgg
     2101 ttcggttcgc agaggccaaa cgcccgccgc gtgaggggcc gcacgaagga attatcggcg
     2161 cactcgtttt cggttcggac caaggtctcg tcggcaggtt taacgaggtg gtcgtagaat
     2221 tcgccgtaaa caagctcaaa acgttgcccg gcaaaacaac aaaaatctgg gcggtcggcg
     2281 atcgcgctca cgcgctcatg gtggactccg gcatggcccc ggttaaactg ctatcagttc
     2341 cgacctcagt caatgccatc acgccgctta taggccagat tctcatcgaa atcgagg
//
LOCUS       DATMGX010000279         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_298331, whole genome shotgun sequence.
ACCESSION   DATMGX010000279 DATMGX010000000
VERSION     DATMGX010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298331"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VNR65_04515"
     CDS             complement(<1..1008)
                     /locus_tag="VNR65_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ17975.1"
                     /translation="MESSEQAGLTSETKAQSGAYPWLRSYPKQIDWFQPFNPAPLTSF
                     LEQAQARFPARPATEFFGKTLSYAELADAVNRATKGLQGLGVGKGTCVGLLFPNCPAF
                     VVFYYAILKAGGIVVGLNPLYAVPELAYQVKDAGADIVITLDLRATFPKAKALLEGGI
                     VKQVVVERFRDSLPGLKGLLFSLFKRKDVASWKAASGVIASRDLLNNDGRFLPCAIDV
                     NSVAALQCTGGTTGTPKGAMLTHSNLSVNVLQVESWFPGLAIDGEERFLCVIPFFHSF
                     AMTGLMNFAIRKAAQMIMLPRFDLKQTLKLIDATRPTIMAGVPTLFNALAKARDIKNH
                     DL"
     gene            1319..1585
                     /locus_tag="VNR65_04520"
     CDS             1319..1585
                     /locus_tag="VNR65_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HWJ17976.1"
                     /translation="MDEVSEKIIAILRKNMRNPEKEIALDTKLADLEIESLDLAVIVF
                     DIEDTFGIQIPYNANEDVVDFATVGSVVDRVKAVLAEQQAPSAA"
     gene            1612..>2332
                     /locus_tag="VNR65_04525"
     CDS             1612..>2332
                     /locus_tag="VNR65_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HWJ17977.1"
                     /translation="MALNGSGRRVAVTGMGVVTCIGTGVDKFWNAVKSGTHGIGTIEG
                     FPLQDLYIKIGGEVRDFDPAERCKSKAVILSDRFSQYASAAAHEAVQQSGLETPFKNG
                     ERAAAIVGSGGGGLSTLEKAYEDIFVHKKKATHPLTLLRTISSTAVAHVSIDHGIKGP
                     VFGTVSACSTGAHAIGLAFQMIKGGAVDVAVAGASEAAINYGSMRAWQAMRVLSPDGC
                     FPFSKRRNGTVLAEGAGILVLE"
BASE COUNT          495 a          679 c          679 g          479 t
ORIGIN      
        1 aagatcgtgg ttcttgatgt cccgagcctt cgcgagcgcg ttaaacagcg tcggaacgcc
       61 cgccatgatg gtcggccttg tcgcatcgat gagcttcagc gtctgtttca ggtcaaagcg
      121 cggcagcatg atcatttgcg ctgccttccg gatcgcgaaa ttcatcaaac cggtcatcgc
      181 gaatgaatgg aagaacggga taacgcagag gaaacgctcc tcaccgtcga tggcgaggcc
      241 cggaaaccag ctttcgacct gcagcacatt gacggagaga ttggaatgcg tgagcatcgc
      301 gcccttcggc gtccctgtcg ttccgccggt acactgcaag gcggcgacgc tgttcacgtc
      361 gatggcgcag gggagaaacc tgccgtcatt attcaggagg tcgcgcgatg cgatgacgcc
      421 ggaggcggct ttccaggacg caacgtcctt ccgtttgaag agactgaaaa gaagcccctt
      481 caggccaggc aaactgtcgc gaaaacgctc cacgaccacc tgcttcacaa tcccgccctc
      541 gagcaaggcc ttcgccttcg ggaaagtcgc cctaagatcg agcgtaatca caatgtcggc
      601 gccggcgtct ttgacctggt atgccagttc agggacggca tagagcggat ttagaccgac
      661 gactatcccg ccggctttca ggatcgcata gtaaaagacc acaaaagccg gacaattcgg
      721 gaacaagagc ccgacgcacg tccccttccc tacgccaagc ccttgaagcc cctttgtggc
      781 gcggttgacg gcgtccgcga gttccgcata gctcaaggtc tttccgaaga attcagtcgc
      841 gggtctcgcc gggaagcggg cctgcgcctg ttcgaggaaa ctcgtcagcg gtgccgggtt
      901 gaaaggctgg aaccagtcta tttgcttcgg gtaggacctc aaccatgggt atgcgcccga
      961 ttgagctttc gtctcgctgg tcaatccggc ttgttcggag ctttccatgg cccttccacc
     1021 ctaagcttct gattgaatat attgaacagt aaggatggag cgtaacgcgg gcgcgtcaag
     1081 gccgaaacgc cacgataaga accagagcgc cgtcgcttct cgtttttcga taggtgagtg
     1141 gcgactttca accacatcat ggtcatgtat tcgccctctc attgaacgaa ggtgttcatc
     1201 tttagggcgt ctcgctggac ttgcggcagg tttgccgcta tctcgttcgc cacgtcttga
     1261 ttgaatgagt gaggcacctt agccttcgcc cgctcgaaaa tcgcccgagg gagccgccat
     1321 ggacgaggta tccgaaaaga ttatcgcaat cttgcgcaag aatatgcgca atcccgaaaa
     1381 ggaaatcgcg ctcgatacca agctcgcaga ccttgaaatt gagtctctgg accttgccgt
     1441 cattgttttc gatatcgagg atacattcgg cattcagatt ccctacaacg cgaatgaaga
     1501 cgttgtggat ttcgcaactg ttggttccgt tgtcgatcgc gtgaaggccg tcctggccga
     1561 gcagcaggca ccgagcgccg cctgactggc gcgcataaca aggcatcgat catggcactg
     1621 aacggcagcg ggcgccgcgt cgcggtcacg ggcatgggcg tcgtgacatg catcggcacg
     1681 ggcgtcgata aattctggaa cgccgtcaaa tccggcacgc acgggatcgg taccatcgaa
     1741 ggcttcccct tgcaggacct ttatatcaag atcggcggcg aggttcgcga tttcgacccg
     1801 gccgagcgct gcaaaagcaa ggccgtgatc ctgtccgatc gcttttcgca atatgccagt
     1861 gccgccgccc acgaagccgt gcagcaatcc gggctggaga cgcccttcaa gaatggagaa
     1921 cgcgctgccg ccatcgtcgg ctcgggcggg ggcggcctat cgacgcttga aaaagcctat
     1981 gaagacatct tcgtccacaa gaagaaagcg acgcatccgc tgacgcttct acgcactatc
     2041 agcagcacgg cggttgcaca cgtcagtatc gatcacggca tcaaagggcc ggttttcggc
     2101 accgtcagcg cctgctcgac cggagctcac gccatcggcc tcgccttcca gatgatcaaa
     2161 ggcggcgctg tcgatgtcgc cgtcgcgggc gcttcggagg cagcgatcaa ttatggctcc
     2221 atgcgcgcgt ggcaggcaat gcgcgtgctc tcgccggatg gctgtttccc gttttcgaaa
     2281 cggcgcaatg gaactgtgct tgccgaggga gcgggaattc tcgttctgga ag
//
LOCUS       DATMGX010000280         7684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_70691, whole genome shotgun sequence.
ACCESSION   DATMGX010000280 DATMGX010000000
VERSION     DATMGX010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70691"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(570..2297)
                     /locus_tag="VNR65_04530"
     CDS             complement(570..2297)
                     /locus_tag="VNR65_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWJ17978.1"
                     /translation="MHDLAFRPYAAVVALSLLCCALFLPGIATLPVTDRDEARFAQAS
                     KQMVETGDTIDIRLQDKPRYKKPAGIYWLQAAAVTVATRAGAALNDIWAYRIPSFLGA
                     LGAVLLTFWAGRAILGREAALIASALFATTLMLSFEARIAKADAALIASITLSQAALF
                     RLYEAPAKAATRGLATLFWFGLGAGILIKGPVALGLALLTSVVALAADARRGWLKNLH
                     WRWGVPLLLLVTLPWLIAIGIASNGDFFTQSVTQDFAGKLQAAQEKHWGPPGFYGLAF
                     LWTFWPAALFVTADTIRALWKMRRSRRIIFLFAWIVPFWLILEAIPTKLPHYALPLYP
                     AIAMVAAFAMRSEKPPQIRVSAIIWGLVAATQITLLLCMSWIAEAPQLTLLAAICAIF
                     AATAALTVIAAWRSYRSLALFGIILSGAVFYGAGYRIALPALEPFWISEKASAAAHAL
                     EGCGRGPAGFAGLNEISVVFQNGTDTLLTAPGTLADALATNQVRVAFVSWKNEHEFEQ
                     AFSQRAGHGPEFFGCVDGIDINGQGPVRLQVYVRPEAANAPGCAPTPAMACRPKDAVR
                     WRRLLDTKF"
     gene            complement(2442..2669)
                     /locus_tag="VNR65_04535"
     CDS             complement(2442..2669)
                     /locus_tag="VNR65_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17979.1"
                     /translation="MSLPDTRALVNLALAGKLDSVSIERDPIFERAVPTAAEGATKTP
                     RVSMFEENFKKFQAYVEPGVNMTAQALRAAE"
     gene            3188..3886
                     /locus_tag="VNR65_04540"
     CDS             3188..3886
                     /locus_tag="VNR65_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ17980.1"
                     /translation="MPTIALVDDDRNILTSLRMLLEAEGYKIMAYTDGVSAFDGIMGD
                     PPDLAILDIKMPRMDGMELLRRLRQKSEMPVIFLTSKDEEIDELFGLKMGADDFIRKP
                     FSQRLVVERVRAILRRSQPRDASAAAVEDSSKIIERGKLKLDPERHTCIWDGKQVTLT
                     VTEFLILQALAARPGVVKSRDALMDAAYDDQVYVDDRTIDSHIKRLRKKFKVVDNNFD
                     VIETLYGVGYRFKE"
     gene            3964..5829
                     /locus_tag="VNR65_04545"
     CDS             3964..5829
                     /locus_tag="VNR65_04545"
                     /inference="COORDINATES: protein motif:HMM:NF025131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HWJ17981.1"
                     /translation="MAIDMSRWWEGIGVRRPSRAIRLRLLRAAHHFFDRYRFNSITRR
                     IIVINVIGVGVLVSGIFYLNQYRYKFMGNRVETLTTQAQIIAIAIAQTSKTNLDAAAE
                     NGKAADENKIVVPSGLSNPELSFRVDPEVVSPILRDLIAPTKTRARVFDTDGTLISDS
                     RQLYRGNGSRMEGSTAETDTNYFTDLWAYIKTAFWTPDLPLYKDLGPEGKAYEEVRVA
                     LAGSVAPLIRVTEAGETVISIAVPIQRERSALGALLLTAQGSDIDEMIAQDRFQIVEV
                     AGLVAFVTAILSLLLAGTIAEPMRRLAIAAERVRKNIKNREQIPDFSDRPDEIGHLSR
                     AFRDMTNALYSRLDAIESFAADVSHELKNPLTSLRSAASLLPMIKTDEDRKKLVDIIN
                     HDVRRLDRLITDIADASRLDAELARETARPVNLANLLRTLCEVMRESRRGSGTNFVLN
                     VESCKTTKNFAQNRDYIVNGHDSRLSQVIANLLENAMSFSPKNGSIYVTARRLPNENE
                     IEICIEDEGPGIRDENLEKIFDRFYTDRPGAFGQNSGLGLNISQQIVKAHGGSIWAEN
                     RASPKPIRFAPERGPARVEELVPVPKGEQTFACGAKERHSWGARFVIRLPACSAK"
     gene            5845..6306
                     /locus_tag="VNR65_04550"
     CDS             5845..6306
                     /locus_tag="VNR65_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HWJ17982.1"
                     /translation="MKRQTVHGTCVALGNAAALLRGSSGSGKSDLALRFLYLPSDRLG
                     AEPALVADDQVILRRDRDHVVASCPERLVGKIEVRGCGIAKLKASAAQATLKLIVDLD
                     WTGDRPRFPEKTEWETVLDIPVRRLVLDPFEPSAPIKLALAIQGCFEDTVD"
     gene            6417..6818
                     /locus_tag="VNR65_04555"
     CDS             6417..6818
                     /locus_tag="VNR65_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWJ17983.1"
                     /translation="MIGLIIVTHGNLAREFVAALEHVVGPQQQIAAISIAPNDDMEER
                     RDAIIAAVKTVESGDGVIILTDMFGGTPSNLAISVMDSGSVEVIAGVNLPMLVKLARV
                     REEVALVDALKQAQEAGRKYIHVASLVLSAN"
     gene            6841..7176
                     /locus_tag="VNR65_04560"
     CDS             6841..7176
                     /locus_tag="VNR65_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HWJ17984.1"
                     /translation="MSLRPSFPSRRRPTIAAADGIYQCLAEVTIVNKRGLHARASAAF
                     VKMAEQFDAEVSVTKDDQTVPGNSIMGLLMLGASPGTMISIETEGPEAEEALEALTAL
                     VEAGFNESE"
     gene            complement(7183..7410)
                     /locus_tag="VNR65_04565"
     CDS             complement(7183..7410)
                     /locus_tag="VNR65_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17985.1"
                     /translation="MRIFAALALTFALLSQAHAQSGGSVYHIAQICAFDIQQYCKGIS
                     PRRIRDLKECLAKNERNLLPRCQDHYKEARP"
BASE COUNT         1667 a         2160 c         2295 g         1562 t
ORIGIN      
        1 ttattcctgc cgcaatcact tcctgccaag cctgcttcct ggccggaagt aaatccaata
       61 gaccgtggaa aataaagggt aatccgtaac ctgtgacctc ccattaaata gaatttaatg
      121 cgggaatttt ctcctttact gggacgctct aaagtccgtt aaatatgttt cggccgccct
      181 aattaaccaa gatcccgccc ttcgcggcca cgggggcgcc atctttatgg ttactttttc
      241 ctgaagattt aaagggacat aaagccatgt cagctcgcgg caaggcgctt ttttccgaga
      301 tcccagccca ggcaatccag agcgcacaat cctgagttga gaagagggat gctccccttc
      361 aacgatgcca acaaaagctc gaccgccgca gatggcgcat accggcgctg gaggcttggg
      421 cttcattggg gggcagagag gaagcatcca gcgccgggtc tgcgtagaac gcaagttctg
      481 cctgagctca aaggcggggt tggggtggaa gcgtggcggc ttcatgaaat tttgtcgtca
      541 gttgtcgtca cggatcaaga ggcggttgcc tagaacttag tgtcgaggag ccggcgccag
      601 cgcacggcgt ccttgggccg gcacgccatg gcaggggtag gcgcgcagcc tggtgcgttc
      661 gccgcttcgg gtctgacata gacttggaga cgaacggggc cttgcccgtt gatatcgatg
      721 ccgtcgacgc atccgaaaaa ctcaggcccg tggccagccc tttgggaaaa ggcctgctcg
      781 aactcgtgtt cgtttttcca ggagacaaaa gccaccctca cttgattcgt ggcgagggcg
      841 tcagccaggg taccaggcgc tgtcagaagc gtatccgtcc cattctggaa aacgacgctt
      901 atctcgttga gcccggcaaa gccagcaggc cctcttccgc agccttcgag cgcatgcgcg
      961 gcggcggaag ccttctcgct gatccagaag ggttcgaggg cgggaagggc gatgcggtat
     1021 ccggcgccgt agaacacagc accgctgagg attatcccga atagcgcgag gctccgatag
     1081 gaccgccatg cggcgataac ggttagcgcg gccgtcgcgg caaagatggc gcatatcgcg
     1141 gcgagcaggg tcagctgcgg cgcttccgcg atccaggaca tgcaaaggag aagggtgatc
     1201 tgagtcgctg ctacgaggcc ccagatgatg gcgctgacac gaatttgcgg cggcttttcc
     1261 gatcgcatcg cgaaagccgc gaccatcgca attgcggggt aaaggggcag cgcataatgc
     1321 ggaagtttcg tcgggatcgc ctccaggatg agccagaacg ggacaatcca ggcgaaaagg
     1381 aaaatgatgc ggcgagagcg tcgcatcttc caaagggcgc ggatggtgtc agccgtcacg
     1441 aacagggcgg ctggccagaa agtccacagg aaggcgagac catagaagcc cggtggcccc
     1501 caatgctttt cctgggcggc ttgcagtttc ccggcgaaat cctgcgtaac cgactgcgtg
     1561 aaaaagtcac cgttcgaagc gatgccgatt gcgatgagcc agggaagagt gacgagaagg
     1621 agcaggggga cgccccatct ccagtgcagg ttcttgagcc agccgcggcg cgcgtcggcg
     1681 gcgagcgcga cgaccgaggt cagtaaggcg aggccaagcg cgacaggtcc cttgatgagg
     1741 atgccggctc caagtccgaa ccagaacaac gtcgccagcc cacgggtcgc agctttcgcc
     1801 ggagcttcgt agaggcgaaa cagcgcagct tgtgagagcg tgatgcttgc aatcagagct
     1861 gcatccgcct ttgcgatgcg cgcctcgaat gaaagcatca gtgtcgtcgc gaaaagcgcg
     1921 gatgcaatca aggcggcttc ccgaccgagg attgcgcggc ctgcccagaa tgttagaaga
     1981 accgcaccga gcgcgccaag aaatgaggga attcgatagg cccatatgtc gttgagcgcg
     2041 gcgcccgctc ttgtggcaac ggtcacagcc gcagcctgca gccagtatat ccctgcgggc
     2101 tttttatagc gcggcttgtc ctgaaggcgg atgtcgatgg tgtcgccggt ctcgaccatc
     2161 tgctttgagg cttgagcgaa tcgcgcctcg tcgcgatccg ttaccggaag cgtagctatg
     2221 cccggcagga agagcgcgca gcacagaagt gagagcgcaa ccaccgccgc atagggccgg
     2281 aaggccaggt catgcaaaaa agccgccgat tttgcatcgg cggcccaaag ccaaggaaat
     2341 cgtcctacgg tacgccgttc attgttagac aggagcgctc gcataacaac ggcagtacgc
     2401 gccgattcgg gatgcacgca acctttggta gagatcgcct cctattcggc ggcgcgcaaa
     2461 gcttgcgccg tcatattgac accgggctcg acataagcct ggaatttttt gaagttctcc
     2521 tcgaacatcg aaacgcgggg cgtcttagtt gccccttccg cagccgttgg aaccgcgcgt
     2581 tcgaaaatag ggtcgcgctc tatggaaacg gaatcaagct tgcctgcaag cgcgagattg
     2641 acaagcgccc gcgtgtcggg aagggacatg cgggccggta tgcgttccag taacgacggc
     2701 gaaggcccag tcgacgtgac ttgccccttt ccacggcgaa tcgcttgctc ttaccgggtc
     2761 gcaacgggag catttgcaac aaagcgctgc gcagatcggg cgccatgatg ctgttattgt
     2821 cgtggatatg attcgaagaa ggcacggccc acctcgaaac gaactttatt gctgctggcg
     2881 tccgacggga aacatggcgt ataaatcaca actcgcgaaa ccccgaacgg aagccttaca
     2941 tttcgcctat aaggcctgga aaaacgcgaa ttggcgtgct tcgcaacctc atcggcctgg
     3001 caaggaaagg cggcttgcca accccagtcc cgcgacggtt agcttaacgg agccttaatt
     3061 ggaaattgac cattgggacg caattcagcc atgatctgcg tgcgctgctt gcgcctcacg
     3121 gatttttccg gctagaggta tttcggcaac agaaacgcct cgttgaacca acaaccttgg
     3181 attactgatg ccaactatcg ctcttgtcga tgatgaccgg aacatcctga catcccttcg
     3241 gatgcttctc gaggccgaag ggtataagat catggcctat acggacggcg tttccgcttt
     3301 tgatgggatc atgggcgatc cgccggatct cgcgatcctc gacatcaaga tgcctcgcat
     3361 ggatgggatg gagttgctcc ggcggctgcg gcaaaaaagc gaaatgccgg tgatctttct
     3421 gacctcgaag gatgaggaga tcgacgagct gttcggcctc aagatggggg ctgacgattt
     3481 catccgtaag ccgttctctc aacggctggt cgttgagcgt gtgcgtgcga tcctgcgccg
     3541 cagccaaccg cgtgatgcgt ccgccgccgc cgtggaggac tcctcgaaga tcatcgagcg
     3601 tggcaagctc aagctcgatc cggagcgtca cacatgcatc tgggatggca agcaggttac
     3661 gcttaccgtg accgagtttc tcattttgca ggcgttggcg gcgcggcccg gcgtcgtgaa
     3721 aagccgcgat gcgctcatgg acgcagccta cgacgatcag gtttatgtgg acgaccgcac
     3781 gatcgacagt cacatcaaga ggctgcgcaa gaaattcaag gttgtcgaca acaacttcga
     3841 tgtgatcgag accttgtacg gcgttgggta tcgcttcaag gaataaggct caatgttgcg
     3901 ggtcttctaa gaaggtgaaa tagtttccag ggcgcgagcc ttcgttcgga cccgagttag
     3961 ccaatggcca ttgatatgtc ccgatggtgg gaagggattg gggtacgccg cccgagccga
     4021 gcgatacgct tgcgcctcct tcgtgccgcc caccactttt ttgatcgtta tcgcttcaac
     4081 agcatcaccc gccgcatcat tgttatcaat gttattggcg ttggtgttct cgtaagcggc
     4141 atcttctatc tcaatcaata ccgctacaaa ttcatgggga accgcgtgga gacgctcacc
     4201 acgcaggccc agatcatcgc aatcgcaatt gctcagacga gcaagaccaa tcttgatgca
     4261 gccgccgaaa acgggaaagc cgccgacgaa aacaagattg tggtgccttc gggcttatcc
     4321 aatccggagc tgtccttccg ggtcgatccg gaggttgtct cgcccattct gcgcgacctg
     4381 atcgcgccca cgaaaacgcg agcgcgcgtg ttcgacacgg atggaacgct tatctctgac
     4441 agccgccagc tctaccgtgg caatggcagc cgcatggagg gaagtacggc cgagaccgac
     4501 accaattact tcacggacct ttgggcttat atcaaaactg cgttctggac gccggacctt
     4561 cccctctaca aagatctcgg ccccgagggt aaggcatatg aggaggttcg tgttgctctc
     4621 gccggcagcg tcgcgccgct catccgcgtg accgaagcag gggaaacggt gatttcgatc
     4681 gctgtcccaa ttcagcgtga gaggtcggcg ctcggcgctc tcctgctgac ggcgcagggc
     4741 agcgacatcg atgagatgat cgcgcaggat cgatttcaga tcgtcgaagt cgcaggcttg
     4801 gtcgcctttg tgacggccat tctgtcgctg cttctggcgg gcacgattgc cgagcccatg
     4861 cgacgcctcg ccattgccgc ggagcgcgtg cgaaagaaca tcaagaaccg ggagcagatc
     4921 cccgatttta gcgacaggcc cgatgagatc ggtcatttgt cccgagcgtt tcgggatatg
     4981 acaaatgcgc tctactcccg tcttgacgcc atcgaaagct tcgcggccga cgtgtctcat
     5041 gaactcaaga atccgctgac atcgcttcgc agcgccgcca gtctgctgcc catgatcaag
     5101 acggacgagg atcgaaagaa gcttgtcgac attatcaatc acgacgtgcg gcgtttggac
     5161 cgcctcatca ccgatatcgc cgacgcttcg agactcgacg ctgaacttgc tcgcgaaacg
     5221 gccaggcctg tgaacttggc gaaccttctc cgcacccttt gtgaagtcat gcgggagagc
     5281 cgccgcggca gcggcacaaa ttttgttttg aacgtggaaa gctgcaagac aacgaagaat
     5341 tttgcgcaga accgggacta catcgtcaac ggacatgaca gccggctcag ccaggtgatc
     5401 gcgaatctcc tcgaaaatgc catgtcgttc tcgcccaaga acggaagcat ctacgtcacc
     5461 gcgaggcgcc tgccgaacga gaacgagatc gagatttgca tcgaggatga ggggcccggc
     5521 atccgcgatg agaatctgga gaagattttc gatcgcttct acacagaccg gcccggagct
     5581 tttggccaga attccggtct cggcctcaac atctcgcagc agatcgtcaa ggcacatggc
     5641 ggaagcatct gggcagagaa cagagcctct cccaagccga taaggttcgc tccggaaagg
     5701 ggcccggcgc gcgtcgaaga actggtgccc gtgcccaaag gtgaacagac gtttgcatgc
     5761 ggtgcgaagg agcgccattc gtggggtgcc cgttttgtga tccgcctccc cgcatgctct
     5821 gcgaagtgat aggggcgaaa gcccatgaaa agacagaccg tgcatggaac ctgcgtcgcg
     5881 cttggcaatg cagccgcgct cctcagagga tcgagcggat cgggcaagtc cgacctggcg
     5941 ctgcgtttct tgtatcttcc ctccgatcgt ctcggcgcgg agcccgctct cgtggctgac
     6001 gatcaggtca ttctacggcg ggaccgcgat catgtcgtcg cttcctgccc tgagaggctg
     6061 gttggaaaaa tcgaggtgcg agggtgcggt attgctaagc tcaaagcgtc cgcggcgcag
     6121 gctacgctga agcttatcgt cgatctcgat tggaccggcg acagaccgcg tttccccgaa
     6181 aagaccgagt gggagactgt gctcgatatc cctgtccgcc gcctcgtcct cgatccgttc
     6241 gagccttctg cgccgatcaa gcttgctttg gcgatccagg gttgctttga agacacagtg
     6301 gattaacagg ctcttatcat cacggcgact atattgcgct tgaacgttga agggttgggc
     6361 ctcagaataa tgctgttgag atgtataccc accgctgcca gtcacctgga aagacgatga
     6421 tcggcctcat cattgtaact catggcaacc tggcaaggga atttgtcgca gccctcgaac
     6481 acgtcgttgg gcctcagcag caaattgcag ccatctcgat cgccccgaac gacgacatgg
     6541 aagagcgccg ggacgcgatt atcgccgcgg ttaagacggt tgagagcgga gacggcgtta
     6601 ttattctgac agatatgttt ggcgggacac cttcaaatct ggcaatatcg gtgatggaca
     6661 gcgggtcggt ggaggtcatc gcgggcgtca atctccctat gctcgtcaag ctggcgcgcg
     6721 tccgcgagga ggtcgcgtta gtcgacgctt tgaagcaagc gcaggaagcg ggccggaaat
     6781 acatccacgt tgcgagcctg gttctaagcg cgaattaaca agaatggtgc cgacctatcg
     6841 atgtcccttc gaccgagttt cccaagtcgc agacgtccga ctatcgcggc ggctgacgga
     6901 atctatcagt gccttgccga agtgacgatc gtcaacaagc gcggactgca tgcgcgagct
     6961 tcagcggcct tcgtgaagat ggctgagcaa ttcgacgctg aagtttctgt gacgaaagac
     7021 gatcagacgg tgcccggcaa ttccatcatg ggtcttctga tgcttggcgc gagccccgga
     7081 accatgatct cgatcgagac cgaggggccg gaagccgaag aagccctcga agcgctcacc
     7141 gcgctcgtcg aagcgggctt caacgaaagc gagtgatccg ctttacggtc ttgcttcctt
     7201 gtagtgatcc tggcagcggg gcagcaggtt tctttcattc ttggcgagac actccttcag
     7261 atcacggatc ctcctgggag aaatgccctt gcagtattgc tgaatgtcga aagcgcagat
     7321 ttgggcaata tgatagacac tcccgccact ctgggcatgg gcttgggaca acagtgcaaa
     7381 tgtgagcgcc agcgcggcaa aaatcctcat ggatgctccc gatggatcgt ggtgaaggtg
     7441 gttgcgtagg gcgaggcttg ggcgaaagtt ggacagccgc acttcgctag agcctaagtt
     7501 ttttgtttgt ctccagccat agcttggtcc gttaccctat ctaagcgcgc ctaattctcc
     7561 tggcttttct tatcctgatc gccttgatcg ggatgtcccg gatgcgcctc gctctgagct
     7621 ttcgcgagcg cgctctgtac ctcgctcata gtcgcaggca tgacctcgga catataggtc
     7681 ccca
//
LOCUS       DATMGX010000281         2892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_133285, whole genome shotgun sequence.
ACCESSION   DATMGX010000281 DATMGX010000000
VERSION     DATMGX010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133285"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VNR65_04570"
     CDS             complement(<1..1551)
                     /locus_tag="VNR65_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ17986.1"
                     /translation="MTTAPSSASRRRIATISATVARAQPNLYDFIVFVAIGAIFVAIV
                     HGAKSMHRPLEVLEANPVSLDPRNLPQYALLTTLRMFAALGFSLLFTFLVAPLAAKSQ
                     KAERVIVPALDILQSVPVLGFLTFTVVFFMNLFPGSQLGAELAAIFAIFTSQAWNMTF
                     SFYQSLKTVPRDLAEITIHLGLSPWQRFWKLDAPFATPGLVWNMMVSMSGGWFFVVAS
                     EAISVGDTKITLPGIGSYLSLAISEKNIGAVAWAVGAMGVVIMLYDQLLFRPIVAWAD
                     KFSVELVAAQEQPRSWVLDLLRRTRLVQAITGFVSGLLDMFSGNVRQLAKQIPEPKVN
                     RVASKVFDWAYNLALLGVVAWALYSVISYVSATLGWPDVQEAVTGGFLTFFRVIILIA
                     LASLIWVPIGVYIGLRPAMAERIQPLAQFLAAFPANVLFPFVVIGIVTYNLNPDIWLS
                     PLIVLGTQWYILFNVIAGASVFPGDLREASKIYQLRSWLWWRKVILRGIFPYYVTGAL
                     TASGGSWNA"
     gene            2361..>2892
                     /locus_tag="VNR65_04575"
     CDS             2361..>2892
                     /locus_tag="VNR65_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWJ17987.1"
                     /translation="MPDELVFYTNPMSRGRIARWMLEEIGQPYRTENIEFGPAMKSPE
                     YLAINPMGKVPAIRHGDTVVTETGAICAYLADAFPQAGLAPEPGSRERGSYYRWLFFG
                     AGPVDGAIVNKSLGFEPPAERQGMVGYGTLAYVLNTLEKAVSASDYLVGDKFSAADLY
                     LGAQLGWGMMFGALEKR"
BASE COUNT          664 a          810 c          870 g          548 t
ORIGIN      
        1 tgcgttccat gagccgccgg aagccgtcag cgcgcccgtg acatagtagg gaaatatccc
       61 acgcagaatc actttgcgcc accacagcca gctcctcagc tggtaaatct tcgaggcttc
      121 ccttaggtcg cccggaaaca cgctcgcacc cgcgatgacg ttgaacagaa tgtaccactg
      181 tgtgccgagc acgataagcg gcgacagcca aatgtccgga ttgaggttat aggtgacaat
      241 gccaatgacc acgaagggga agagtacatt ggcaggaaac gcggcaagga attgagccag
      301 aggctgtatg cgctcggcca ttgccggccg cagcccgata taaacaccaa tcggcaccca
      361 gatcaggctt gcaagggcga tcaggatgat gacgcggaaa aaggtcagga agccgcccgt
      421 cacggcttcc tgaacgtcgg gccagccgag agtggcgctc acataggaaa tcaccgaata
      481 aagggcccaa gctacaacgc caagcagtgc aagattgtag gcccagtcga agaccttgct
      541 tgcaaccctg ttcactttgg gttccggaat ttgcttcgcg agctgcctga cattcccgct
      601 aaacatgtcg agcaggccgc tcacgaaacc ggtgatggct tgaacaaggc gggtccgtct
      661 tagaaggtcc aagacccacg atctcggctg ctcttgcgca gccaccagct caacgctgaa
      721 tttatcggcc caggcgacta tggggcggaa gaggagctga tcgtagagca taattacgac
      781 gcccatcgct ccgactgccc aagccaccgc cccgatattc ttctcgctga tcgccaagga
      841 tagataggag cctataccgg gcagggtgat tttcgtatct ccgacagaga tcgcctcgga
      901 cgccacaaca aagaaccatc cgcccgacat ggataccatc atgttccaga cgagacccgg
      961 agttgcgaag ggcgcatcaa gcttccagaa tcgctgccac ggcgaaagcc ccaaatggat
     1021 cgtgatctcc gccaggtctc gcgggaccgt cttcaacgac tgatagaacg agaaggtcat
     1081 gttccaggcc tggctcgtga agatcgcaaa gatggccgca agttctgcgc cgagttggct
     1141 ccctggaaac aggttcatga agaaaacgac cgtgaaggtc agaaatccga gaaccggcac
     1201 cgattgcagg atgtcgaggg ctgggacgat gacgcgctcg gccttctggc ttttggccgc
     1261 gagcggtgcg accaggaacg tgaaaaggag ggagaagccg agcgctgcga acatccgcag
     1321 cgtggtgaga agcgcatatt gaggcaggtt tcgagggtcg agagagacgg gatttgcctc
     1381 aaggacctca agcgggcggt gcatcgattt cgcgccgtga acgatggcga cgaatatcgc
     1441 gcctatcgcg acgaaaacga tgaaatcgta aaggttgggc tgggcacgcg cgacggtagc
     1501 ggagatggta gcgatgcgcc tgcggctggc ggaagatggg gccgtggtca tggctcgcct
     1561 cgctcctctg atccgtgggc gctttagccc gttgtcattt ttcgcgctcg tttacagtcc
     1621 gcgtctgcgg agacttcgtt tcaagttttt gggttacgcg aagcggatgt tgctgcgctc
     1681 ctaagccgat gaacggcgcg gcgctttctg tcaacacgaa aacgccgcat tggggctcgc
     1741 ggatacaatt tggttctttt gccggggcct aaatatgttg gcttgctcgg actaaatctt
     1801 gaggccgccc ggtttttgaa gcggaagaag ggcgctgtgc atcgcaaagg aaaaaaaagc
     1861 cctcgctgga ggcttttggc ttacgctgga ctcattgcat ttggcggtag cgcgccgtta
     1921 ggttggaacg tgccctccgc tgccggcgag cctcccttac actccgtacg gcccctggcg
     1981 cagcgtggga gcgcaggcat tgatgaggaa accactgacg caaaacactt tgcggagcgc
     2041 ctgaagcacc atcaaagacg ccacttcaca aaacacaggc gcgactttcg caagctgcag
     2101 gcaacgctgg aaagtcagcg gcgattgagg cgggctaagt tgaaagcgcg ccaattcgct
     2161 gcggcacgca ggcatgcagc gcagcgcgcc cacaagcaca agtagatgca cgcaaccaaa
     2221 aagtgcggcg cggccttaac acaattaatc ctgttggaaa cgtcatcgac gggctatgac
     2281 tcaccgtatg gtgattgttt ctgcctcttt ggaacagcca tagtctcaca tccttgcatt
     2341 acaaagaggg agaaccgagg atgcctgacg agctcgtttt ctacaccaat ccaatgtcac
     2401 gcggccgcat tgcccgttgg atgctggagg aaatcggcca gccttaccgc accgaaaaca
     2461 ttgaatttgg accggccatg aagtcgcccg agtacctggc catcaatccg atgggaaagg
     2521 tgccggcaat ccgtcatggc gacaccgttg tcacggagac gggcgcgatc tgcgcttacc
     2581 tcgccgatgc cttcccgcaa gcgggactgg cccctgagcc gggaagccgg gaacgcggct
     2641 cctattaccg ctggctattt ttcggcgcgg gcccggtgga cggggcgatc gtcaacaaaa
     2701 gcctcggttt tgagcccccg gccgaaagac aaggaatggt gggctacggc acgctggcct
     2761 acgtcctgaa cacgttggag aaagcggtct cggcttcgga ttatcttgtg ggagacaaat
     2821 tctccgccgc cgatctgtac cttggggctc agcttggatg ggggatgatg ttcggcgcgc
     2881 tcgagaagcg cc
//
LOCUS       DATMGX010000282         2878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_232725, whole genome shotgun sequence.
ACCESSION   DATMGX010000282 DATMGX010000000
VERSION     DATMGX010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232725"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..2689
                     /locus_tag="VNR65_04580"
     CDS             113..2689
                     /locus_tag="VNR65_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWJ17988.1"
                     /translation="MALTSGTKLGPYEIQSPLGAGGMGEVYKARDTRLDRTVAVKILP
                     AAVSSDPDRLLRFQHEARVLSTLNHPNVLAIFDVGEQAGVQYLISEFLEGHSLRDVLT
                     AGTLSRRAATAYALEIAKGLAAAHEKGVVHRDLKPDNIFITRDDRVKILDFGLAKQAP
                     PQPSAQESQTMTVATTPGTVMGTVGYMSPEQVRGETVDHRSDIFSFGAVLYEMVSGKR
                     AFRGDSSVETMNAILKEDVPELSGPSAQVSPGLDRIVRRCVEKKPERRFQSASDLAFA
                     IESLSGASSAPSASAMTRISEPPKTRRAWLLWVAAALGALGLVAAVWTVDRRSAARPQ
                     PKFTRLTYQRGYPSSARFAKDGQTVVYSAQWNNDPLQVYSVRTEFPQSTKVDLPSAAL
                     LALSSSGDMEVAVDLVQEAWFMYGTMGQAQMTGGTPRAQAKEVIAADYAPDGKTLAIA
                     RRANRKVQLEYPEGKVIYTTAGYLDYVRVSPSGKEVAFAEHPVYGDDRGWISFVDETG
                     NHKQLTQEFGTVQGLSWSRAGTEIWFTAADSTTDRELYGVSLAGKQRTILAAPQSIRL
                     LDIGADGRVLLSVERQEAEIIGIDPASGKQRRGLEWFDASLMGDILPDGNAIVFLEWG
                     GPAGPLYLQVYRKLDGSAPVALGPGAQPRFSPDGTWVAGPLLTRPTQVALNPIGAGES
                     RRLAAGEITSFRTVSWFPDGKHLLVTGAAGEQPVRTYEMDIEGATPQALGPADFSGVT
                     VAIDGKRIAGRNAAGEAVVFDRETQKLEVIPGIEPQEVLSKWTEDGQALITYSSTPSA
                     ASIYRVDTVTGRRTLLQRIDPTEKAGLAKPITLAYAERSKSYAYSTLRVLGNLYVVEG
                     LE"
BASE COUNT          626 a          836 c          870 g          546 t
ORIGIN      
        1 ccttcttcaa cagtcttgcc cgaggcgttt ccgatcaagg ctagcgacta cccgtccata
       61 atcgtcgcat ccggcttgta acgtgcagca caggctataa ttgcgcccac gcatggccct
      121 gacctctggc acaaaactcg gcccctatga aatccagtcg ccgcttggcg ccggcggtat
      181 gggcgaagtg tacaaggctc gtgacacccg cctcgaccgc actgtagcag tcaagatcct
      241 tccggcggcc gtctccagcg atcccgaccg gcttctccgc tttcagcacg aagcccgcgt
      301 cctgagcacg ctcaatcacc ccaatgttct cgctatcttc gatgtcggcg agcaagcggg
      361 cgttcagtat ttgatttcag aattcctgga agggcattcg ctgcgcgatg tgctgacggc
      421 gggaacgctc tcgcgccgcg cggcaacggc gtacgccctc gaaatcgcaa aaggactggc
      481 cgccgcgcac gaaaaaggag tcgtgcaccg cgacctgaaa cccgacaaca tctttattac
      541 acgcgacgat cgggtgaaga tcctcgattt cggtctggct aagcaggctc cgccgcagcc
      601 ctccgcgcaa gaatcgcaga ccatgacggt tgcgaccaca ccgggcacag tcatgggaac
      661 ggtcggatac atgtctcccg aacaagtgcg cggcgagacc gtcgatcatc gttccgacat
      721 cttcagtttc ggcgctgtac tctacgagat ggtctccggc aagcgggcat ttcgtggcga
      781 ctcgtccgtt gagacgatga acgccatcct gaaagaagat gtgccggagt tgtcggggcc
      841 gagcgctcag gtgtctcccg gcctcgaccg catcgtccgt cgttgtgtcg aaaagaagcc
      901 agaacgccgc ttccagtcgg ccagcgatct cgcgtttgcc atcgaatcgc tgagcggagc
      961 gtcgtcggca ccttctgcca gcgcgatgac acgcatctct gagccgccga agacaaggcg
     1021 agcttggctg ctgtgggtcg cggctgctct gggcgcgcta ggattggtcg ccgcagtgtg
     1081 gacagtcgac cggcgctccg cggcaagacc acagccaaaa ttcactcggt tgacctatca
     1141 gcgaggctat ccctcgagcg cgcggtttgc gaaggatggg caaacggtcg tctacagcgc
     1201 gcagtggaac aacgacccac tacaggtcta ctccgtccga acggagtttc cgcaatccac
     1261 taaagtagat cttccgagcg cggctctgct cgcgctttcc tccagcggag acatggaagt
     1321 cgcggtggat ctcgtgcaag aggcgtggtt catgtatgga actatggggc aggcgcagat
     1381 gacaggaggt acgccccgcg ctcaggcaaa ggaggtcatc gcggcggact atgcgcccga
     1441 cggcaagacc ctggcgatcg cgcgtcgagc caaccggaag gtccaactgg agtatccgga
     1501 ggggaaggtt atttatacaa ccgcaggcta cctcgactat gtaagggtgt ctccatccgg
     1561 caaggaagtc gcatttgcgg aacatccggt gtacggcgat gaccggggct ggatatcgtt
     1621 cgtcgatgag acaggaaatc ataagcagtt gacccaggaa tttggaaccg tgcagggttt
     1681 gtcatggtcg cgggccggca cagaaatctg gtttaccgcc gctgatagca cgacggatcg
     1741 ggagctttac ggtgtgagcc ttgctggaaa acagcgaacc attctagccg caccgcaaag
     1801 tattcgcctt ctcgatattg gagcggacgg ccgggtcctt ttgtccgttg agcggcaaga
     1861 agcagaaatc atagggatcg acccggcaag cggcaagcaa cggcgtggac tggaatggtt
     1921 tgatgcttcc ctcatgggcg atatattgcc ggacgggaac gcaattgtat tcctggagtg
     1981 gggaggacca gctgggccgc tttaccttca ggtctaccgc aaactggacg gctctgcgcc
     2041 tgtggcgctg ggaccaggag cgcaacccag attttcgccg gatgggacat gggtcgccgg
     2101 ccccctgctg accaggccca cgcaagttgc cctgaacccg atcggagcag gagagagccg
     2161 ccgcttagcg gcgggagaga tcacaagctt tagaaccgtg tcttggttcc ccgacggaaa
     2221 gcatctgttg gtgacgggcg cggccgggga gcagcccgtg cgcacttacg agatggatat
     2281 tgaaggggcc acgccgcaag ctttgggccc tgccgatttt agcggcgtca cggttgccat
     2341 agatgggaag cggatcgccg ggcgtaatgc cgctggtgaa gctgtggtgt ttgaccggga
     2401 gacacaaaag ctcgaagtca tcccgggcat tgagccccaa gaagtattgt ccaaatggac
     2461 cgaagacgga caggccctga tcacctactc atcgacgcct tcggcagcga gcatctaccg
     2521 cgttgatacg gttactggcc ggcgcacttt attgcagagg attgacccaa ccgaaaaagc
     2581 aggtcttgct aagcccataa cgcttgccta cgctgagcgt agcaagagct atgcatacag
     2641 cacgcttcgg gtactgggaa acttgtacgt ggttgaagga ttggaataaa agactaatcc
     2701 gccacggcgc ccctcgagaa ttctctcgat tcgcccaagt aacggacaaa catgaagtaa
     2761 tcccaaggga agggtagccg acaaaccatc ccgagtcgtc gtgtcagtgc acagcattga
     2821 gcgcagagac ccggctgatg gccaggatac tcacgcggcc gatcgcaaca acgctcaa
//
LOCUS       DATMGX010000283         2525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_107102, whole genome shotgun sequence.
ACCESSION   DATMGX010000283 DATMGX010000000
VERSION     DATMGX010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107102"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VNR65_04585"
     CDS             <1..1188
                     /locus_tag="VNR65_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HWJ17989.1"
                     /translation="LRRSLEPVQANLLTSEDVLLALFQQRIDHLTQFSCVWFVAAARL
                     GFYFADMVRWRLEHHLGEPALAQKLFQGLEGSLITRQAIELENLAHGRISREAFLRAY
                     GHSSTNELEISLPRLSEDPRKTERLLQDLAVSGRQPSDEFQEQQRRRRDTERDVRSRL
                     ETRGASTADLRAFFSDLRLAQRFLPLRETIKHYYTGEYRVLRETLLEINRRLGWKDGD
                     IFYLDPSEISGCFHSRELLAARVRKRRRHRKIAALLAGQHRVPPVVFASNTGALGSQP
                     KSLQSRHLKGVPVAPGSAAGLVRVLDDTGSEPWQHKAMRGDEIIVARSANLGLAPLMR
                     MAAGLVVEVGGVLAHAACQARESGIPAVVLAGATSMLREGMAITIDGATGRIELLEER
                     TAS"
     gene            1185..1997
                     /locus_tag="VNR65_04590"
     CDS             1185..1997
                     /locus_tag="VNR65_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ17990.1"
                     /translation="MTGGLFSLAGRAAVVTGAGANGGIGHAIAVGFARAGADLLICDI
                     DAEGLSRTAEELSPLGALAGAELCDISKAAEVEKLFATADSVLGGVDILVNVPFYFPQ
                     RTAPHEVALEDWERMFAVNVTGYFLCIREAIKRMLAEGQGGAIINIGSNAGETALGRG
                     AFPYSCTKSAIHQMTKELAVEYGAKGIRTNAVLPAQVLTPGLKAHLENPHFRDQVLPR
                     ILMGLPMGRLLSPEDCVGPAIFLASDAAASVNGVLLPVDGGNLAMNAGGSHG"
BASE COUNT          501 a          737 c          760 g          527 t
ORIGIN      
        1 ctgcgccgct cgctggagcc ggtgcaggcg aacctcctca catccgagga tgtcctgctg
       61 gccctctttc agcagaggat agatcatctc acgcaattca gttgcgtctg gttcgttgca
      121 gcagcgcgcc tcggcttcta ctttgcggat atggtgcgct ggcggcttga gcaccatttg
      181 ggcgagcccg cgcttgccca gaagctcttt cagggtctcg aaggcagtct catcactcgg
      241 caggccatcg agctcgaaaa tctggctcat ggccgcatct cgcgggaggc attcttacgc
      301 gcgtacgggc acagttcgac caatgagctg gaaatctcgt taccgcgatt gtcagaagat
      361 ccccgaaaaa ccgagcggct gcttcaagat ctggcggtct cgggacggca gccaagtgat
      421 gaatttcaag aacaacaacg ccgccgccgc gatactgagc gcgatgtgcg cagcaggttg
      481 gagacgcggg gagctagcac ggcagacctt cgcgcatttt tctctgattt gcgccttgcg
      541 cagcggttcc ttccgctccg cgagaccatc aagcactatt acactggcga atatcgggtc
      601 ttgagagaga cccttctgga aatcaaccgc cgtctcggat ggaaggacgg cgatattttt
      661 tacctcgatc cttcggaaat ttccggctgc tttcattcgc gggagttgct cgctgcgcga
      721 gtacgaaagc gccgacggca ccgaaagatt gccgcccttc tggccggaca acatcgggtg
      781 cccccggtcg ttttcgccag caacacgggc gctcttggtt cgcagccgaa atcgcttcaa
      841 tcgagacact tgaaaggggt gccggtggcc ccgggaagcg ctgcaggatt agtgcgcgta
      901 cttgacgata caggcagcga gccctggcag cacaaagcga tgaggggcga tgaaatcatc
      961 gtcgcccgtt ccgcaaatct cggtctcgcg cccttgatgc gaatggcggc tgggctagtc
     1021 gttgaggtcg gcggcgtgct cgcccatgcc gcctgccagg cgcgggaaag cggcattccg
     1081 gccgttgttc tcgccggggc aacctctatg ctgcgggaag gcatggcgat caccatcgac
     1141 ggcgcgaccg ggcgcatcga acttcttgag gaacggacgg catcatgacg ggcggactgt
     1201 tcagtttggc ggggcgcgcg gctgtcgtga caggcgcagg cgcgaacggc ggcatcggtc
     1261 acgcgatcgc cgttggcttt gcgcgcgccg gggcggatct tttgatctgc gacatcgatg
     1321 ccgaggggct ctcccggacc gcggaggagc tgtcacctct tggcgcactg gcgggcgccg
     1381 aactgtgcga catctcgaag gcggcggagg tcgagaagct tttcgccacg gccgacagcg
     1441 tcctgggcgg cgtcgacatt ctcgtcaatg ttccatttta ctttccgcag cgcacggcgc
     1501 cgcacgaggt cgcgctcgaa gattgggaac gcatgttcgc cgtcaacgta accggctact
     1561 tcctctgcat tcgtgaggcc ataaagcgta tgctcgcgga agggcagggc ggggcgatca
     1621 tcaacatcgg ctcgaacgcc ggggagacag ctctcggccg aggcgcgttt ccgtatagct
     1681 gcaccaaaag cgccatccat cagatgacca aggaactggc ggtggaatat ggagcgaagg
     1741 ggatccgcac caatgccgtc cttcccgcgc aggttctcac gccaggactt aaagcgcacc
     1801 tcgaaaaccc tcatttccgt gaccaggttt tgccgcgcat tttgatgggg ctgccgatgg
     1861 gccggttgtt gtcgcctgag gattgtgtcg gccccgcgat cttcctggcg tccgatgccg
     1921 cggcctccgt caacggggtg cttcttcccg tcgatggcgg caatctcgcc atgaatgccg
     1981 gaggcagcca cggctaagcg gccctgaaaa gtgcgaagcg gttttccggt aatgacgcac
     2041 gacaaaacga aaacttagtg ccggcgcgtt aagcgcgggg cgtaggcatt aatccttcgt
     2101 tgtagggacg gtgatggtta acccctggat ggttaatttt gcgcgcccgg atttatttct
     2161 ggtctaaatt gcacccaaca ttggttaatg cgacgttcag cgaagcgcag cccgcgcctc
     2221 gctgaacgta gataagtcgc tggaaacgct caagtttttg agccccaaaa acagggttaa
     2281 ccgaatgtat ttattccaga agtgggacgc gctaaagtcc gttaaatata ttcgcgccgc
     2341 gccattaacc gtgcccgcgc ccttcacacc cgctgcttct tgaaatttta cttataaata
     2401 tcaaggtctt acttccatta acctcccggc aaggcgcatt tccgtatgat caacggttct
     2461 tgagatcaag cggcttgatc ccgacgaact gcaaacaatc tggaccagtc ccgatgaacc
     2521 aaaga
//
LOCUS       DATMGX010000284         2879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_107053, whole genome shotgun sequence.
ACCESSION   DATMGX010000284 DATMGX010000000
VERSION     DATMGX010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107053"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2034
                     /locus_tag="VNR65_04595"
     CDS             <1..2034
                     /locus_tag="VNR65_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWJ17991.1"
                     /translation="VRRWDGQWRLFSIRAVPTFDADGRLREWVGVHTDISKLRSNERA
                     LRDLTATLEQRVEAAIAERDRTWNNARDLLLVTDAMGTFRAANPAWTAILGWRPEEVI
                     GRRDLDFVHSDDRASSTASLATTRGRELKGHEIRMRHKDGSYRWISWVAAPEGGLIYA
                     NGRDVTAEKAAAEAFERSESRFRAIFQTSYQYKGIVALDGTLLDANPTSLRGINASLA
                     EVIGRPFWDTPWFTATPGMPEKVLAAWEAAVKGETVRQEILMNLPIGLRCFDFTMRPI
                     RDARGDVVAIVPEAADITARRQAEEALRQSQKLEAIGQLTGGIAHDFNNLLTPIVGSF
                     DLLQRKCRDDPQALRLISGVLQSAERARLLVQRLLAFARRQHLEARAVDLEQLVRGML
                     DLIGRSLGPRIEVRVDMTTDLPAARVDPNQLEVALLNLAVNARDAMPDGGLISISARI
                     AGLEDEMEGRLVPGRYICLSVADNGTGMTPETLARAVEPFFTTKAIGKGTGLGLSMVH
                     GLAAQSGGQLTLESTLGKGTVAALWLPAIDNSRVEIPAATYEAEFPARPLSVLLVDDD
                     DLVRAGAAAMLESTGHTVVHVSSATRALDHLASREHFDVLVTDQRMPCMTGVDLILRA
                     RFMRPDLPAVLITGYTDAIDPDVSTLPVLAKPFRAAELARAVAEAVNHDRGTV"
     gene            complement(2042..2413)
                     /locus_tag="VNR65_04600"
     CDS             complement(2042..2413)
                     /locus_tag="VNR65_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17992.1"
                     /translation="MVDQPQLKGIRVLVVEDDDLSSLVAQEMLVELGCVVAGAETSVD
                     GALATLENGGPFDCVMLDVRLGAELSSDIASKLLKTNVPFFICSGYDIKLPGMNIPVL
                     DKPYTVDGLGKALALALAHRR"
     gene            complement(2687..2874)
                     /locus_tag="VNR65_04605"
     tRNA            complement(2687..2874)
                     /locus_tag="VNR65_04605"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2727..2729),aa:Gly,seq:gcc)
BASE COUNT          597 a          861 c          834 g          587 t
ORIGIN      
        1 gtgcgccgct gggatggcca atggcgcctc ttttcaattc gcgccgttcc caccttcgac
       61 gccgacggac ggcttcgcga atgggtcggc gttcacaccg atatttcgaa attgcgatcc
      121 aatgagcggg cattacgcga cctgacggct accctggagc agcgcgttga agcggcgata
      181 gccgaacgtg accgtacctg gaacaacgcc cgcgatctct tactcgttac cgatgcgatg
      241 ggcacctttc gcgccgcgaa tccggcttgg acggcgatcc tcggatggag accggaggaa
      301 gtcatcggcc gaagagatct cgatttcgtc catagcgacg accgcgcttc gagcacagcg
      361 tccctcgcaa caacgagggg ccgggaactc aagggccatg aaatccgcat gcggcataag
      421 gatggctcgt atcgctggat ttcgtgggtc gcggcccctg aaggcggcct catctacgcc
      481 aatggacgcg acgtgaccgc cgaaaaggcg gcggccgagg catttgagcg gtcggaatca
      541 cgcttccgcg caatcttcca gacaagctat caatacaaag gcatcgtggc tctcgacggg
      601 acgcttctcg acgcgaatcc aacatcgctt cgaggcatca atgccagctt ggcagaggtc
      661 atcggcagac ctttctggga tacgccctgg ttcacggcga cacctggaat gcccgagaag
      721 gtgctggccg catgggaagc ggcggtaaaa ggtgaaactg tccggcagga aattctcatg
      781 aacctgccaa tcgggctgcg ctgtttcgac ttcacgatgc gcccgatccg ggatgcacgg
      841 ggcgatgttg ttgcgattgt gccggaagcg gccgacatca ccgctaggcg ccaggcggag
      901 gaagcgcttc gccagagtca gaaacttgag gccatcggcc agcttaccgg cggcatcgcc
      961 cacgatttca acaatctcct gaccccgatt gtggggagct tcgatctctt gcagcgcaag
     1021 tgccgcgatg atccccaggc cttgcgcctg atctcgggcg tgcttcaatc ggccgagcgt
     1081 gcgcgtcttc tcgtgcaacg tcttcttgct ttcgcgcgcc gccagcattt agaagcccgc
     1141 gcggtcgacc tggaacagct cgtccggggc atgctcgatc tgatcgggcg gtcgcttggt
     1201 ccgcgaatcg aggtccgcgt tgacatgacg acggatctgc ctgccgcgcg cgtcgatccg
     1261 aaccagcttg aggtggctct tctcaacctg gccgtgaatg cccgcgacgc catgccggac
     1321 ggcggcctca tctccatctc ggctaggatc gcaggtctgg aagacgagat ggaaggccgc
     1381 cttgtcccgg gcagatatat ctgcctgtcc gtcgcagata acgggaccgg gatgacaccg
     1441 gagacattgg cgcgggccgt ggaacctttc ttcacgacga aggccatcgg caagggcacg
     1501 ggtctcgggc tttccatggt tcatggccta gccgcccaat ccggcgggca attgacgctt
     1561 gagagcacac tcgggaaagg gacagtggcc gcgttgtggc tgcctgcgat tgataattcg
     1621 cgggtcgaaa tcccggctgc aacctatgaa gccgagttcc cggcacgacc cttgtctgtc
     1681 ttgcttgtgg atgacgacga tcttgtgcgg gcgggggctg cggcgatgct ggaatcgact
     1741 gggcatacgg ttgtgcacgt gtcttccgcc acgcgcgcgc tcgatcatct tgcctcccgc
     1801 gaacacttcg acgtacttgt taccgatcag agaatgccgt gcatgacggg ggtcgatctc
     1861 attttgcgcg cacgattcat gcgccccgat cttcctgccg tcctcataac cggctacacc
     1921 gacgctattg atccagacgt gagcacactg cccgtgcttg cgaagccgtt tcgcgctgcg
     1981 gaactcgcgc gcgcggtcgc tgaggcagtc aaccatgatc gcggaacagt gtgaggtaca
     2041 gctatcgcct gtgcgccaaa gcgagggcga gcgccttgcc gaggccatcg accgtatacg
     2101 gcttatccag cacggggata ttcattcccg gcagcttgat gtcgtagccg ctgcaaatga
     2161 aaaagggaac attcgtcttc aaaagcttcg aggcgatatc gcttgaaagc tcggccccaa
     2221 ggcgaacgtc aagcataacg caatcaaatg ggccgccatt ttcaagcgtg gccagcgccc
     2281 catccaccga cgtttcggcc ccggcaacga cacagccgag ctcgacaagc atctcctgcg
     2341 cgacgagaga agaaaggtca tcgtcttcga caaccagaac gcggatgccc tttagctgcg
     2401 gttggtccac cattccgtct cctgccccct gaaacgcaag ctgaacgatc gaaactttca
     2461 gctttgatca attctcattc ttggccctca agtcaacatg tggcgccggt ttatttctag
     2521 aattgcaatt atctattttg ttagatcgag cctttgcgaa gtgccatagt aagacacaag
     2581 ctcgctctcc tccgctgaga gtggtctcgt ccaatgcgaa gttaaaaatt ttgcgatttg
     2641 agagaaaagt ccgcagaacc tggaaggaac tgcgaaatga tcaagctgga gcgggcgagg
     2701 cgattcgaac gcccgaccct aaccttggca aggttatgcc agcataattg atcacaatcg
     2761 atcacggtgg atttgtcata aaaaacagca ccttgatatt cacgcttcca ccttcatcca
     2821 ttgacttaaa cgccgcgtaa tggcatttat ccgctacccc agcgctaccc cagcggcgg
//
LOCUS       DATMGX010000285         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_137038, whole genome shotgun sequence.
ACCESSION   DATMGX010000285 DATMGX010000000
VERSION     DATMGX010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137038"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /gene="ptsP"
                     /locus_tag="VNR65_04610"
     CDS             <1..1830
                     /gene="ptsP"
                     /locus_tag="VNR65_04610"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HWJ17993.1"
                     /translation="EEVEALETTAMVLAEMIASGTFAGAVAPAPSRRETSVTLKGTPL
                     ADGLALGHIALHEPRPAVLKFFAENIPAELKRLDEALDRVKEDIARLLLDDTPASNGE
                     HKEIIETVQMLAHDRGWARRMRDAVSAGLTAEAAVERVQQNMRTQLMKTGESFWRERS
                     HDLDDLSNRLLRILQGHDPGAALSRLPNDAILVAHNMGAAELLDYDPKKLRGLVIEDG
                     GATSHVSIVARSFNLAVLVQVHDVVAAADHGDPAILDASTGQFHIRPSLELIRAYADK
                     ARFRAKRQARFFKLRHAPAVTKDGERVVLNMNAGLLVDMPHLYQSGADGIGLFRTELQ
                     FMVASKFPKMEEQRAAYTRVLDMAPGRRVVFRSLDVGGDKVLPYLRKFHEENPALGWR
                     AIRMSLDRPGMFRTQVRALLRAAAGRELSVMLPFIADLSELVAARDLIDKELEHSAMH
                     GHELPVKTRVGVMIEVPSIIYQLDDLLPLVDFVSVGSNDLLQFLFAADRENDRVARRF
                     DSLNPSALKVLRKIAERAQTHGTDFALCGEMAGRPLEAMALIGLGFRQISMAPASIGP
                     VKSMVLSLNAKKLRETLFELIDRNSPKIRQELAEFAQKENIDI"
     gene            complement(1897..>2129)
                     /locus_tag="VNR65_04615"
     CDS             complement(1897..>2129)
                     /locus_tag="VNR65_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17994.1"
                     /translation="AIVLRALEGLDGGYQRIKLRKLLRELDKGFVIDPGGKLRLNRLP
                     TADETIEFLLWNRCHRFLLRRVSTRNRLKTES"
BASE COUNT          419 a          646 c          638 g          426 t
ORIGIN      
        1 gaggaggtcg aggcgctcga aacgacggcg atggtactgg cggagatgat cgcgtccggc
       61 acctttgctg gagcggtcgc acccgcgcca agccgccgcg agacgtcggt caccctgaaa
      121 ggaacgcctc ttgcggatgg ccttgcgctc ggtcacatcg ctctgcacga gccccggccg
      181 gccgtcctca agttcttcgc ggagaacatc ccggcggagc tgaaacggct ggatgaggcg
      241 ctcgaccggg tcaaagagga tatcgcccgc cttctcttgg acgatacgcc tgcttcgaac
      301 ggcgagcaca aggagatcat cgaaacggtg caaatgctcg cccacgatcg gggctgggcg
      361 cgccgaatgc gcgatgcggt ctcggccggc cttacggcgg aggcggcggt cgagcgcgtg
      421 cagcagaata tgcgcacgca gctcatgaag accggcgaaa gcttctggcg cgagcgctcg
      481 cacgacctcg acgacctttc caatcggctg ctccgcattc tgcaggggca cgaccctggc
      541 gcggccttga gcaggctgcc gaatgacgcc atcctcgtcg ctcacaacat gggagctgcc
      601 gagcttctcg attacgatcc caagaagttg cgcggtctcg tgatcgaaga tggcggcgcg
      661 acctcccacg tttccattgt cgcccgctcc ttcaatctcg ccgttcttgt ccaggtgcac
      721 gacgtcgtgg cggctgccga tcacggagac ccggcgatcc ttgatgcctc caccgggcag
      781 tttcatatcc gcccgtctct ggagcttatc agagcctatg ccgacaaggc gcgcttccgg
      841 gccaaacggc aggctcgctt ctttaagctg cggcatgcgc cagctgttac gaaggacgga
      901 gagcgtgttg ttctcaacat gaatgccgga cttctcgtcg acatgccgca cctttatcaa
      961 agcggggcgg acggcatcgg gctgttcagg accgagttgc agttcatggt cgcgtcgaag
     1021 ttccccaaga tggaggagca gcgcgcggcg tatacacgcg tgctcgacat ggcgccaggc
     1081 cgacgcgtcg ttttccgatc cctcgatgtg ggcggcgata aggttctgcc atatctgagg
     1141 aagttccatg aggagaaccc cgcgctcggg tggcgggcga tccgcatgtc gctcgacagg
     1201 ccgggaatgt tccggacgca ggtgcgggct ttgctgcggg cggccgcagg gcgggagctg
     1261 tccgtcatgc tgccgttcat cgccgatctc tccgagcttg tcgctgccag agacctcatc
     1321 gacaaggaac tcgagcactc ggcaatgcat ggtcatgagc tgccggtcaa aacccgcgtc
     1381 ggcgtcatga tcgaggtccc ctcgatcatt taccagctgg atgatctttt gccgcttgtc
     1441 gacttcgttt ccgtcggtag caatgacctc cttcagttcc ttttcgccgc ggaccgggaa
     1501 aacgatcgcg tcgcccggcg cttcgattct ctgaatccct cagcgctcaa ggttttacgc
     1561 aaaatagccg aacgggcgca aacgcacggc acggacttcg ctctctgcgg ggaaatggca
     1621 ggtcgtccct tggaagcgat ggccttgatc gggcttgggt tccgccaaat atccatggcg
     1681 ccggcatcga ttggcccggt taagtccatg gtgctgtcgc tcaacgcaaa gaagctgcgt
     1741 gaaacgttgt tcgagcttat cgaccggaac agcccgaaga ttcggcaaga actcgcagag
     1801 tttgcccaga aagaaaacat tgacatttaa cgtcgcactt agagcagccc gcgtccattt
     1861 gtatgcggat aagctgctct agcactctag aatcgatcaa ctttccgtct ttaatcggtt
     1921 ccgagtagag acgcggcgga gcaaaaatct atggcatcta ttccacaaga gaaactcaat
     1981 cgtctcgtca gccgttggta aacgattcaa acgcagcttg cctccgggat cgatcacgaa
     2041 acctttgtca agctctcgaa ggagtttgcg gagcttgatc cgctggtaac cgccatcaag
     2101 gccctccaaa gcgcgcagca cgatcgcga
//
LOCUS       DATMGX010000286         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_55412, whole genome shotgun sequence.
ACCESSION   DATMGX010000286 DATMGX010000000
VERSION     DATMGX010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55412"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            632..1651
                     /locus_tag="VNR65_04620"
     CDS             632..1651
                     /locus_tag="VNR65_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWJ17995.1"
                     /translation="MRSLFSMFSSDLAIDLGTANTLVYARGKGIVVNEPSIVAINKNT
                     GEVEAVGKEAKEMLGRTPGNIVAIKPMKDGVIADFKVTEKMLNYFIQKAHNRKMLVHP
                     RIVIGVPSEITQVEKRAVMDSAYRAKASEVHLVEQAMVAAIGAGLPITEPSGNMVVDI
                     GGGTTDVAVISLSGIVYSRSVRMAGNQMDESIMNYLKRKYNLLIGERTAEQIKIEIGS
                     AYPLDKPLTMEIKGRNLIEGVPKTITIDDSEIRESLSECVSTVMNAIRVALERTPPEL
                     SADISDRGIVLTGGGALLKNLDKRIREETGLPVSIADDPLCSVVLGTGKMLSDFKLLR
                     KISID"
     gene            1812..>2215
                     /locus_tag="VNR65_04625"
     CDS             1812..>2215
                     /locus_tag="VNR65_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17996.1"
                     /translation="MDTFLGRYKNLIVLAAILFAQIIALAVQVRRPTQGGDTRLLRLW
                     AISAVTPIEKAVVHSQKWVYDTWNTYAYLRGVRRENRQLRAEIERMKVEDARFAEDAR
                     MARRIQALLAFKEQYVDTTVAAQVIGTSGSEQS"
BASE COUNT          508 a          641 c          623 g          443 t
ORIGIN      
        1 agcccctgag ggaattcgca cttagtctca accccgaagt ccctcagtgg ctaaagccac
       61 cacgatgaca gcgcttacgg cacggctgaa aagaggctgc tgaaaaatga accaaagtag
      121 taatttcgag cgtttcttga ctcaaaagtc actcgtaagt tattgaatcg tcgttgcgca
      181 aaatgccgag tttacttgtt tgagtctttt ttcagcagcc tctgaagccg tgccccttca
      241 aagcgtgaga tcctcatgaa taatccaggt gaaggcccgc actgcccggg ccagcggatg
      301 cactaatcct ggaaccgatc tggccttgcc tattggcgca aaagtaacat tctcctgtgc
      361 tactctcaag gtggtactct gatttgccaa cagtgggtag aagcccgcta tttcgcgtcg
      421 cgaaagagtg gagcgcaact ggaacggaat tccatccggc ggttaacctc gaaaaacaaa
      481 cctcgttatc tgccggactc atacattttg gttgactgcg cgtgagtgcc gcttctgtca
      541 taaactgtat gcagcaacct tcatacttag aaagaaatag ctcgtaatgt catctaacgg
      601 ctttcatagt agcgcctcgc gattcagcaa catgcgctcg ctgttcagca tgttctccag
      661 cgatctggcc atcgacctgg ggacggcgaa cacgctggtg tacgcgcgcg gcaaaggcat
      721 cgtggtcaac gagccttcca tcgtcgccat caacaagaac acgggtgaag tagaagccgt
      781 gggcaaagag gccaaggaga tgcttggccg cacccccggc aacatcgtcg ccatcaagcc
      841 catgaaagac ggcgtaatcg ccgacttcaa agttaccgag aagatgctga actacttcat
      901 ccagaaggcc cacaaccgca agatgctggt gcatccccgc atcgtgatcg gtgtcccttc
      961 ggagatcacg caggtggaaa agcgcgcggt catggactcc gcctatcgcg ccaaggccag
     1021 cgaggtccac ctggtggagc aggcgatggt ggccgcgatc ggcgcgggcc tgcctatcac
     1081 cgagcccagc ggcaacatgg tggtcgatat tggcggcggc accaccgacg tcgcggttat
     1141 ttcgctcagc ggcatcgtgt attcgcgctc ggtccgcatg gccggcaatc agatggacga
     1201 gtccatcatg aattacctga agcgcaagta caatctgctg atcggcgagc gcaccgccga
     1261 acagattaag atcgagatcg gctccgccta tccgctggac aagccgctca ccatggagat
     1321 caagggccgc aacctgatcg agggcgtgcc caagaccatt acgattgacg acagcgagat
     1381 ccgcgagtcg ctcagcgaat gtgtttccac ggtgatgaac gcgatccgcg tcgcgctgga
     1441 gcgtacgcct ccagagttgt cggccgacat cagcgatcgc ggcattgtgc tgaccggcgg
     1501 cggtgcactg ctgaagaacc tggacaagcg cattcgcgag gaaaccgggc tgccggtctc
     1561 gattgcggac gacccgctat gcagcgtggt gctgggcacg ggaaaaatgt tgagcgactt
     1621 caagctgctg cggaaaatct cgattgacta gcagcgggca tgtcccttcg gcggcgcgag
     1681 tctattttga attgtcatca cgagcgcagc gagggatctg cttttctcga caggcgcagg
     1741 aaaaaagcag atgcctcgct ccgctcgtca tgacaattca agtacttgcg ttccccgaag
     1801 cagcgtagtg tatggataca ttcctcggcc gctataaaaa cctgattgtg ctggcggcca
     1861 ttctgttcgc ccagattatc gctctggccg tgcaggtgcg ccgcccaacc caaggtggcg
     1921 acacccgcct cctccgcctg tgggcgatct ccgccgtgac tccgatcgaa aaagcggtgg
     1981 tgcactcgca gaaatgggtg tacgacacct ggaacaccta cgcctatctt cgcggagttc
     2041 gcagggaaaa ccggcaacta cgcgccgaga tcgagcgcat gaaggttgaa gacgcccgct
     2101 tcgccgagga tgctcgtatg gcgcgtcgca tccaggcatt gctcgcattc aaggagcagt
     2161 acgtcgatac caccgtcgct gcccaggtca tcggcaccag cggcagcgaa caatc
//
LOCUS       DATMGX010000287         2480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_387909, whole genome shotgun sequence.
ACCESSION   DATMGX010000287 DATMGX010000000
VERSION     DATMGX010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387909"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..618)
                     /locus_tag="VNR65_04630"
     CDS             complement(148..618)
                     /locus_tag="VNR65_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17997.1"
                     /translation="MAANSQAICDTAKTDFMNGLHAFGASVIRAGTTKDTFYAALFVT
                     NNSMGAATTAYSSSGEVTNGSGTGYTAGGLAVTNANPPSIYTHTASWTPSASFVWTAF
                     TSAAAFDTCLLYNNTASGKNAVAVYCFGAQSITSGTFTLSQPTDGAATGLLRIA"
     gene            complement(631..843)
                     /locus_tag="VNR65_04635"
     CDS             complement(631..843)
                     /locus_tag="VNR65_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ17998.1"
                     /translation="MALILTTHGDMDEALLEKKEGVVDNEQEHTSWVEYWLPNGADGK
                     PELVHRSASVQLKRLPSLFGKQESFR"
     gene            complement(856..1308)
                     /locus_tag="VNR65_04640"
     CDS             complement(856..1308)
                     /locus_tag="VNR65_04640"
                     /inference="COORDINATES: protein motif:HMM:NF040141.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HWJ17999.1"
                     /translation="MTTFTLARKPDDGENERTRGLLTCPAVPEADALKIVTLERPWLN
                     NQPKVSCIPAGTYQVVISLSPKFGRLMPRLLNVPGRSEIECHWGNVVTDTEGCIIVGT
                     SETPYGVAWSRLAFGKFFPWLTAALKSGDVFIAITNSAPEPMLAAQAA"
     gene            complement(1305..1625)
                     /locus_tag="VNR65_04645"
     CDS             complement(1305..1625)
                     /locus_tag="VNR65_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18000.1"
                     /translation="MLVVFGLIAVLFVIGFLVVKRDQKRADERRAAEVLRLECEDREF
                     ERKAAKAASLAALQRTVNDIRAERAAKIEAAGLVPAVPPTGTPERRMPRSLPKKKAKK
                     GRGR"
     gene            complement(1626..1793)
                     /locus_tag="VNR65_04650"
     CDS             complement(1626..1793)
                     /locus_tag="VNR65_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18001.1"
                     /translation="MTILPLVLVLAFVLLCTVAAIRTGHGRGPTKEEIIQRAREWKLG
                     HMVDPPRKKGE"
     gene            complement(1790..2209)
                     /locus_tag="VNR65_04655"
     CDS             complement(1790..2209)
                     /locus_tag="VNR65_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18002.1"
                     /translation="MKLDVSLAQQIVAVLTVLATVLFALRKLTMAARIGRVSMTNEIE
                     TLSKLNASMREELRKENESLRARVNLLEEHVSDLAAEVQKLQDLKALLEKERDDLKAE
                     IRMLKRALTAEQFADVGSTGAPGARGEKGDKGDKGDK"
     gene            complement(2199..>2480)
                     /locus_tag="VNR65_04660"
     CDS             complement(2199..>2480)
                     /locus_tag="VNR65_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18003.1"
                     /translation="EPTNYVWAVDRVLQHGHFGIILLAVGLFQTAANLGWHPRARAIS
                     VFLAFLAWSGLSAIGFLAAHDFLVVPLATAFAITQGIVFVHLEKVPREA"
BASE COUNT          443 a          803 c          712 g          522 t
ORIGIN      
        1 taagcgagcc agcgccggcc gtgatgccgg acgcgagcgc ggacttggcg aaatttacgg
       61 cgagatccat ttagacggca ctcgccggtc gcgcgagaaa ctctacgtct gtcaatgtgt
      121 gctttcgtcg gtgggaggtg ccatctgcta tgcgatcctc aatagacccg tggccgcccc
      181 gtccgttggt tgactgaggg taaatgtgcc gcttgtgatc gactgcgctc cgaagcaata
      241 gaccgccacc gcattctttc cgctcgcggt gttgttatac aacagacacg tatcaaacgc
      301 ggcggccgac gtgaacgccg tccacacaaa cgatgcgctc ggcgtccagc tcgctgtgtg
      361 tgtgtagatc gacggcggat tagcgttcgt cacggccagg ccacccgctg tgtatccggt
      421 gccagaacca ttcgtgactt caccagatga actgtatgcc gtggtcgcag cgcccatcga
      481 gttgttcgtg acgaacagcg cggcgtagaa tgtatctttc gtcgttccag cgcggatcac
      541 agacgcgcca aaggcgtgaa ggccgttcat gaagtccgtc tttgccgtgt cgcaaatcgc
      601 ctgagaattg gctgccatcg cattctccct ttatctgaaa ctttcctgtt tgccgaacag
      661 agacggtagt ctcttcagtt gtacgctcgc gctccgatgc accagctcgg gcttcccgtc
      721 ggcgccattc ggcagccaat actccaccca agacgtatgt tcctgttcat tatccaccac
      781 gccctctttc ttctcgagca gcgcctcgtc catgtcgccg tgtgtcgtca gaatcagcgc
      841 catacattcc tccgtttagg ccgcctgcgc ggcaagcatt ggttccggtg ccgaattcgt
      901 gatcgcaatg aaaacgtcgc cgctcttcag cgccgccgtc agccacggga agaacttccc
      961 gaacgcgagc cgcgaccatg cgaccccgta gggcgtctcg ctggtcccga cgatgatgca
     1021 cccctccgtg tcggtcacga cgttccccca atggcactcg atctctgacc gccctggcac
     1081 gttgagcagg cgcggcatca accgaccgaa tttaggcgac agcgagatca cgacctgata
     1141 ggttccggcc gggatgcagg agaccttcgg ctggttgttc agccacggtc gctccagcgt
     1201 cacgatcttc agcgcatcgg cttcgggcac cgccggacac gtcagcagcc cgcgcgtgcg
     1261 ctcgttctcg ccatcgtccg gcttgcgggc gagcgtgaac gtcgtcaccg tccgcggccc
     1321 ttcttcgcct tcttcttcgg caaggagcga ggcatccgac gctctggcgt gcccgtgggc
     1381 ggcacggccg gcacgagtcc agcggcctcg atcttcgcgg cacgttctgc ccggatgtcg
     1441 ttcaccgtcc gctggagagc cgcgagcgag gcagccttcg ccgccttccg ctcgaactcg
     1501 cggtcttcac attccagtct cagcacctcg gcggctctgc gttcgtcggc cctcttctgg
     1561 tcgcgcttca cgacaaggaa tccgatcacg aacaagactg cgatgagtcc gaaaacgact
     1621 aacatttact cccccttctt tctaggcgga tccaccatgt gcccgagctt ccactcgcgg
     1681 gcgcgctgga tgatctcttc ctttgtcggt ccacgtccgt gccccgtgcg gatcgccgcg
     1741 acggtgcaga ggaggacgaa cgcgagtacg aggacgagcg gcaggatggt catttgtccc
     1801 ccttgtcccc cttgtccccc ttttcgcccc tcgctcccgg ggcccccgtt gagccaacgt
     1861 cggcaaactg ttctgcggtc agagctctct tgagcatgcg gatctccgct ttgaggtcgt
     1921 cgcgttcttt ctcgagcagc gccttgagat cctggagttt ctggacctca gcggcgaggt
     1981 cgcttacatg ctcctccagc agattgacgc gcgcgcgcag agattcgttt tccttgcgca
     2041 actcctcgcg catcgaggcg ttcagcttgc tgagcgtctc aatttcgttc gtcatggata
     2101 cacgaccgat ccttgccgcc atcgtcagct tgcggagtgc gaaaagaacc gtggcgagaa
     2161 ccgttagaac ggcgactatc tgctgcgcga gtgacacgtc aagcttcacg tggcaccttc
     2221 tctagatgga caaatacgat cccttgcgtg attgcgaagg ccgtcgccag cggaacgacg
     2281 agaaagtcgt gcgcggcaag gaagccaatc gcagagagcc cagaccatgc taggaatgcc
     2341 aagaacacgg atatggctcg tgcgcgcggg tgccagccca ggttcgcggc ggtctggaag
     2401 aggccaaccg cgagcagaat gatgccgaag tgcccgtgtt gcagaacgcg atcgaccgcc
     2461 cagacatagt tcgtcggctc
//
LOCUS       DATMGX010000288         2058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_208280, whole genome shotgun sequence.
ACCESSION   DATMGX010000288 DATMGX010000000
VERSION     DATMGX010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208280"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VNR65_04665"
     CDS             complement(<1..529)
                     /locus_tag="VNR65_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWJ18004.1"
                     /translation="MIVPAISALLVGLLVAACSRQDKAQTVQPPRPVLSVLVEPRTVE
                     MFGPFTGTIQPRYQSSAGFQIAGRMVTRDVNVGDLVKAGQLLASLDPRLTRFSLNSAE
                     ADVANARATLINAQATEDRQKKLLADAMGGTMQAQVDAAIANRQTAQAKLDQGLANLQ
                     KAKEQVGYTELRTDYN"
     gene            complement(544..1629)
                     /locus_tag="VNR65_04670"
     CDS             complement(544..1629)
                     /locus_tag="VNR65_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWJ18005.1"
                     /translation="MSLKRAIAQSLCVVATIVLSAFVSRAEGLERELVRVRTVTAEMS
                     PFSREIQLTGEIQARIQTNIAFRITGKVISRAVDVGDHVTADQVLATLDPRDEKSDAD
                     NAKAAVASADAVLVQSKTNFDRQQSLIASGFTTRAAYDQAKAAFDAATAQVDAANAAL
                     KTVEEQLSYTKLRAGADGIIVARNAEAGEVVQPGRTIFTLAQDGPRDAIFEVYEGLVS
                     GRPRTTPIDITLQSDSKVKAAATVREVSPTVDPKSGTVRVKLGLINAPPEMTLGAAVT
                     GRARFGTGEAIVLPWSALYEWQGKPAVWVLGEGSRVSPQIVTIESFATRELILSGGLK
                     PGDKVVTAGIQFLRPGQTVAVAQGDSP"
BASE COUNT          388 a          653 c          609 g          408 t
ORIGIN      
        1 cattgtagtc cgtacgaagc tcggtatagc ccacctgctc tttcgctttt tgcaagttcg
       61 ccagcccctg atcgagcttg gcttgggctg tctgccggtt tgcgatcgcg gcatcgacct
      121 gcgcttgcat cgtgcctccc atcgcatccg cgagaagctt cttctgccgg tcctcggtgg
      181 cttgcgcgtt aatcagggtc gcgcgcgcat tggcgacatc ggcctccgcc gagttgagcg
      241 aaaagcgcgt gagcctcgga tcgagcgagg ccagaagctg cccagccttc acgagatcgc
      301 cgacgttgac gtcgcgggtc accatccggc ccgcgatttg gaagcccgcg gagctttgat
      361 aacggggctg gatcgttcct gtgaacggac cgaacatctc cacggtgcga ggctcgacaa
      421 gaaccgagag aaccggccgc ggcggctgaa ctgtctgcgc cttgtcctgc cgggagcacg
      481 ccgcaacaag caggccgacc aagagtgccg atatggcggg cactatcacg agccctgatc
      541 gcctcatggg gaatcccctt gcgcgaccgc caccgtttgg cccggacgca ggaactgaat
      601 accagccgtg acaaccttgt cgcccggctt cagaccgccc gacaggatga gttctctggt
      661 ggcgaagctt tcgatggtca cgatctgtgg cgacacgcga ctgccctcgc ccaaaaccca
      721 aaccgcgggc tttccttgcc actcgtacag agcgctccag ggcaggacaa tggcttcacc
      781 tgtaccgaat cgcgcccggc ccgtaactgc ggctccaagc gtcatctcgg gcggcgcatt
      841 aatgagcccg agcttcacgc gcaccgtccc ggacttcgga tccaccgtgg gtgagacttc
      901 gcgcacggtc gcggcggctt tcaccttcga atcggactgc agcgtaatat caatgggtgt
      961 ggtcctcgga cggccgctca ccaggccttc atagacttcg aagatcgcgt cgcggggacc
     1021 gtcctgagca agcgtgaaaa ttgttcttcc gggttgcacg acctccccgg cctcggcatt
     1081 gcgcgccacg atgatgccgt cggcccccgc tcgaagcttg gtgtaggaca gctgctcttc
     1141 cactgttttg agcgcagcat tcgcagcgtc gacttgcgcc gtggccgcat cgaatgccgc
     1201 cttcgcctga tcgtatgccg cccgcgttgt gaacccgctt gcgatgagcg actgctggcg
     1261 gtcgaaattc gtctttgatt gcacgagcac cgcatcagca gaggcaaccg cggctttcgc
     1321 attatcggcg tcggacttct cgtcgcgggg atcgagcgtc gccaaaacct gatcagccgt
     1381 gacgtggtcg ccaacatcaa cggcacggct gatgactttg ccggtgatgc ggaacgcgat
     1441 attcgtttga atgcgcgcct gaatttcgcc cgtcagctga atctcccgcg agaatggtga
     1501 catctcggcc gtaacggttc ggaccctgac aagctcgcgc tccaaaccct cggcgcgcga
     1561 tacgaacgcc gataggacga tggtggcgac aacgcaaagg gattgggcga tcgctcgctt
     1621 caaactcatt ttcccttggg acctttcacg ctgaatggcg ggtattgcgc ggtcccctgg
     1681 aaccgccgcg aacgaccggc ctataaatgc cgcgtcccag gctttacaga caacaaagaa
     1741 atttgcatag ccgggaaccg gttccaatct gaaacatggc gccagcgtat aagaagcatg
     1801 tggccgagcg tcaccggaga acgatgaacg cctccgcgct cgggatcagg ttctgcgtgg
     1861 caggaatagg tgcgatcctc gctgcaactt cgtgcagtag agcctcacgg atatcgcctc
     1921 cgatggcaga gaggtcgtct ggacgcgccc gaggccgaat ctattgaggc gacgaccgcc
     1981 aatctttgct ctttcaactc gcggcatatg cttcaccgga ccggatgcga gttctgagcg
     2041 gagcataatg ccctgccc
//
LOCUS       DATMGX010000289         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_30108, whole genome shotgun sequence.
ACCESSION   DATMGX010000289 DATMGX010000000
VERSION     DATMGX010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30108"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1543)
                     /locus_tag="VNR65_04675"
     CDS             complement(257..1543)
                     /locus_tag="VNR65_04675"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ18006.1"
                     /translation="MMSYRVAAVDVHKRMMAVVVADVEGEGNYQFERRKFGATPGELH
                     VLAQWLDQQGVEEVVMESTAQYWRPLWGTLEQFWRPARQQREGASQMSGTLHLCQAKS
                     NHGPRGRKNDFLDAERMVKRLVAQELVLSFVPDPEQRLLRTVTRRKHQLTRAKVGFKN
                     QLESLLEQGHIKLSSFVSDLLGVSARRMLKALAEGESDPAALAAMADRGLRATPEQLR
                     DALGASAHLSKVYRRLLKLALEELQLIETHIEQLDREAMELLKGHEVVVQKVAEVPGF
                     GPDSAVQMIAEVGIQAANFAAAKKLSSWVGVCPGNEESAGESHSTRSPKGNRHMRRLL
                     NQAAQSAVKVKGSIFEVTFHRLLPRLGYKQAIWAIAHRLCQLLWLILHKGVRYEERGP
                     AVSAKSKRTRAARMIRELKKLGYRVEGGPIPAAARA"
     gene            complement(1802..>2172)
                     /locus_tag="VNR65_04680"
     CDS             complement(1802..>2172)
                     /locus_tag="VNR65_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWJ18007.1"
                     /translation="SLWLFQWMIENKVMDVVQPDLNYNGGFIRAARVARMARKANMWI
                     CPHNTQTGAASVNILQFAATTPNIGPWMEYVSRAAAKKESWYTPNFEIRNGVIPVPTG
                     HGMGVEFDPDFLKKAAVVRA"
BASE COUNT          385 a          717 c          618 g          452 t
ORIGIN      
        1 ctggaacgcc atcgggatga ggttctcgcc attgccgcac ccgatctcca ggacgcggca
       61 gcgccggagc ggggctgggt ccatgccgaa gagaatggcg agggtggcga gccggtcggg
      121 atgggtttgg gaaaacggcg agttgggata cagcacctca tcatagatgg tgggcattcc
      181 cccgagtgta ccgccgtcgg cgggtgggac agattttagc gaagcatgac agggtcgaaa
      241 aaccacccta caccactcat gcccgggcgg cggctggaat tggaccacct tctacgcgat
      301 agccgagttt cttgagttcc ctgatcatgc gggcggcgcg tgttcgtttg gacttggcgc
      361 ttaccgccgg tccccgttcc tcgtagcgga cccccttatg taggatcagc cagagcaact
      421 ggcagagacg atgagcgatg gcccagatgg cctgcttgta tcccaacctc ggcaacaagc
      481 gatgaaatgt tacttcgaat atgcttcctt tgaccttgac cgcggattga gctgcctgat
      541 tcagtagtcg ccgcatgtgt cggttgcctt tcggcgagcg cgtactgtgc gattcccctg
      601 cgctctcttc gttgcccgga cacaccccca cccacgaaga caacttcttc gccgctgcga
      661 aattcgccgc ctgtatgccc acttcggcga tcatctgcac cgctgaatcc ggcccgaacc
      721 ccggcacttc cgccaccttt tgcaccacaa cttcatgccc cttgaggagt tccatggctt
      781 cccgatccag ttgctcgatg tgcgtctcga tcaactgcaa ctcctccagc gccagcttca
      841 gcaggcgacg gtatactttg ctcaagtgtg cgctggcacc cagcgcatcg cgcaattgtt
      901 ccggcgtggc acgcaatccc cgatcggcca tcgctgccag cgccgccggg tcgctttccc
      961 cttccgccag ggccttcaac atccgccgcg cactgacgcc cagaagatcc gacacaaaac
     1021 tcgacagctt gatgtgcccc tgctccaaca ggctctccag ttggttctta aaccccacct
     1081 tggcgcgcgt caactggtgc ttccgccgcg tcaccgttcg caagagccgc tgctccgggt
     1141 ccggcacgaa gctcagcacc agttcctgcg ccaccagtcg cttgaccatc ctttcggcgt
     1201 cgagaaaatc gttctttcgc ccgcgcggtc cgtggttgct cttggcttgg cacaggtgta
     1261 gggtaccgga catctggctg gccccctccc gttgctgccg cgccggcctc cagaactgct
     1321 ccagcgtgcc ccacagcggc ctccagtact gcgcggtcga ttccatgacg acttcttcga
     1381 ccccctgctg gtccagccat tgggccagca cgtgcaattc accgggggta gcgccgaact
     1441 tgcggcgctc aaactgatag ttgccctccc cctccacgtc agccactacg actgccatca
     1501 tcctcttgtg aacgtccact gctgcaactc gatatgacat catgcctctc ctcggcggag
     1561 cggagagcac gcccaaaggt attcccaggc agggtcacgt ggctatcgcc gctcccgtga
     1621 ggaagacgca cgacgcccaa ttattcgcgc agaagcgcag catgctccag ggtcaaacag
     1681 gccaacgatc tcaacgtctt cactgtctag tcgacctcac cgccccacca ttgccatgat
     1741 cccagttttt catgggattt cgcgggccga aggcccattc aaacaggcca ggaggcctgt
     1801 cctacgctct tacgacggcc gccttcttga ggaagtccgg atcgaattcc acgcccatgc
     1861 cgtggccggt gggaaccggg atgaccccgt tgcggatttc gaaattgggc gtgtaccagg
     1921 attccttttt ggcggcggcg cgggagacgt actccatcca tgggccgatg ttcggcgtgg
     1981 tggcggcgaa ttgcaggatg ttgacgctgg cggcgccggt ctgcgtgttg tgcgggcaga
     2041 tccacatgtt ggctttgcgc gccatgcggg ccacgcgggc ggcgcggatg aagccgccgt
     2101 tgtagttgag atccggctgc accacgtcca tgaccttgtt ttcgatcatc cactggaaga
     2161 gccagaggct gg
//
LOCUS       DATMGX010000290         3159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_429303, whole genome shotgun sequence.
ACCESSION   DATMGX010000290 DATMGX010000000
VERSION     DATMGX010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429303"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VNR65_04685"
     CDS             <1..473
                     /locus_tag="VNR65_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWJ18008.1"
                     /translation="RGTHALDDAVAALTKLVGQPEDSKLTAALADPFMFQEYYRDRAP
                     EFLGKEFGPKFALAVTKFSPGSWQGPVESGFGWHLVFVDTLIPGSVPAFEEVEADVKV
                     AWLAEQKAQAWQKTYQDMRAKYTVLLPAPAEGASETGDTSPGKPGPTRSGERGS"
     gene            470..1492
                     /locus_tag="VNR65_04690"
     CDS             470..1492
                     /locus_tag="VNR65_04690"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HWJ18009.1"
                     /translation="MKRWQHCFGWLLAGPLLALLLVPLAASAHEARPAYLAVNETAPG
                     QFSILWRTPVLAGMRLPIVLKLPDRLKNLKDPIVSELSDSLVERRWVDAGLNGLAGER
                     IEFPGLQLTITDVLVRVQMLDGNVSTTLVRPARPWIEVATAPGFLAVAGAYVLHGIEH
                     ILFGFDHLLFVLALILIVRDGRVLLWTITAFTAAHSITLSLATLGVVHVPGPPVEAMI
                     ALSILLLACEIIRLRYGQASLTSRWPWIVAFSFGLLHGFGFASALTDIGLPQGDIPLA
                     LLSFNVGVELGQLMFIGVVLGALFCFRRFGVPPRISQAALTVATYAIGVTAAFWFIER
                     LAGFVS"
     gene            1505..1852
                     /locus_tag="VNR65_04695"
     CDS             1505..1852
                     /locus_tag="VNR65_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18010.1"
                     /translation="MNKVLSFAAVAEAVTGLALLVVPSLVGQLLLGQQLTGIAEPVAR
                     VAGIALIGLGVACWPGPPLLGMLIYSALVTLYLAYLGVAEGLTGVLLWPAAVLHAILT
                     ALLIRDVAGMRRD"
     gene            1824..2258
                     /locus_tag="VNR65_04700"
     CDS             1824..2258
                     /locus_tag="VNR65_04700"
                     /inference="COORDINATES: protein motif:HMM:NF018151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeA/HdeB family chaperone"
                     /protein_id="HWJ18011.1"
                     /translation="MTSPECDATKYGEAKIRNPKQSGKNMRKLLISTATGVVMMIAGS
                     TAKAQVDLSAYADAEGFLDVQALTCAQLANTWQGDADRLTAWYSGWYNGLAKKHYMDI
                     TKGKEAEHEVIVYCKANPEIKIIQAMEIVFKSMRERLGIKVQ"
     gene            complement(2955..>3159)
                     /locus_tag="VNR65_04705"
     CDS             complement(2955..>3159)
                     /locus_tag="VNR65_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18012.1"
                     /translation="KPPEQPKPFPLFWLRTAALRQERSFTRKLCSTNLGGTEIKMPAF
                     LALSWLYRDLSDGSDPSPGYFTS"
BASE COUNT          602 a          903 c          993 g          661 t
ORIGIN      
        1 gccgcgggac gcacgcgctt gacgatgccg tcgccgcgct gacgaaactc gtcgggcagc
       61 cggaggattc caaactcacc gcggcgcttg ccgatccgtt catgttccag gaatactacc
      121 gcgatcgcgc gcctgaattc ctgggcaagg agttcggtcc caaattcgcc cttgcggtga
      181 cgaaattttc gccggggtcg tggcaaggac ctgtcgagtc cggcttcggt tggcatcttg
      241 tgttcgtcga caccctgatc cccggcagcg tgcccgcgtt tgaggaagtc gaggccgacg
      301 tcaaggttgc ctggctcgcc gagcagaagg cacaggcgtg gcagaaaacc taccaggaca
      361 tgcgcgcgaa atacacggtg ctattgcccg cgccggccga gggcgcatcg gaaaccggcg
      421 atacctcgcc cggcaagccg ggtcctactc gatcaggcga gcgaggctcg tgaaacggtg
      481 gcagcactgc ttcggctggc tgctcgccgg gccgctgctg gccttgctgc tcgtgccgct
      541 tgcggcgtcg gcgcacgagg cgcgcccggc ctatctcgcg gtcaacgaga ccgcgcccgg
      601 acaattcagc atcctgtggc gcacgccggt actcgctggc atgcggctgc cgatcgtcct
      661 caagcttccg gaccgtctga agaatctgaa ggaccccatt gtgagcgagc tctcggactc
      721 gctcgtcgag cgtcgctggg tcgacgcggg gctgaacgga ttggctggcg agcggatcga
      781 gttcccgggc ctgcaattga cgatcacgga cgtgctggtg cgggtgcaga tgctcgacgg
      841 caacgtctcg accaccctgg tacggccagc ccgcccatgg attgaggtcg cgaccgcgcc
      901 cggatttctg gcggtggcgg gagcttacgt cttgcacgga atcgagcaca ttctgttcgg
      961 cttcgaccac ctgctgttcg tgctcgcgct tatcctgatc gtgcgcgacg gtcgggtatt
     1021 gttgtggacc atcaccgcct tcacggccgc ccattcgatt acgctcagcc tcgccacgct
     1081 cggggtcgtg catgttccgg ggccgccggt cgaggccatg atcgcgctga gcattctgct
     1141 gctggcgtgc gagatcattc gcttgcggta cggtcaggcc agcctgacgt cgcgatggcc
     1201 atggatcgtg gccttctcct tcggactgct gcacggcttc ggcttcgcca gcgcgttgac
     1261 agacatcggg ctgccacaag gcgacatccc gctcgcgttg ctcagtttca acgtgggcgt
     1321 ggagctgggg cagttgatgt tcatcggcgt cgtccttggg gcattgtttt gcttccgccg
     1381 cttcggcgtc ccgccaagga tcagccaggc cgctctcacg gtcgcgacct atgcgatcgg
     1441 cgtcacggct gcgttctggt tcattgagcg gctcgcaggg tttgttagtt gaagggtctg
     1501 gacgatgaac aaggtgctaa gtttcgccgc tgtcgccgag gctgtaacgg ggctggcgtt
     1561 gctggttgtg ccgtcgctgg tcggacagtt gttgctgggc caacaactca ccggcatcgc
     1621 ggagccagtt gcgcgcgtag ccggcattgc gctgattggc ctgggcgttg cctgctggcc
     1681 gggcccgccg ctcctgggca tgctgatcta cagcgcattg gttacgctgt atctggctta
     1741 cctcggtgtg gcagagggct tgaccggcgt cctgctgtgg ccggcggcgg tccttcacgc
     1801 gatcctgacc gcgctcttga tacgtgacgt cgccggaatg cgacgcgact aaatacggcg
     1861 aagccaagat ccgaaaccca aagcagagtg gaaaaaacat gcgcaagtta ctaatttcga
     1921 ccgcaacagg cgtcgtgatg atgatagcag gttcaacagc gaaagctcaa gttgatctca
     1981 gtgcttacgc tgatgccgaa gggttcctcg acgttcaggc actgacttgc gcgcaacttg
     2041 ccaacacatg gcaaggcgac gccgataggc tcaccgcgtg gtacagcggt tggtacaatg
     2101 ggctcgcaaa aaaacactac atggacataa caaaaggcaa ggaagccgag catgaagtga
     2161 ttgtgtactg caaggcaaac cccgagataa aaatcattca agccatggag atcgtattca
     2221 aaagcatgag agaacgactc ggtatcaaag tccagtaagt gagcgagtgc gatctttggc
     2281 gcgagagcgg gagtgtcact atactgcgga tcagaccgga acggagcgtc gctttcgcca
     2341 atcgatggca agggtatctc cgttccgtgg gcaatcgcag ctagcgcagt tgcggatgaa
     2401 taaaggctgt aggacgaact gctacgtcac tccaaactct caatgttttt cgatctaaag
     2461 gggctatcca tgaaaatata gcaagcggct atttgccatc gtctccgtat tcttcgcagc
     2521 gcttctgtca ttgtgtccgg gaacactcaa tgcgcgcgac gccgatctcg cctctttcac
     2581 cttcgcggca gctcagaccg ctaaacagca atgcatcaat atctgtcgag cgcgatatcg
     2641 cgactgcctg tccatgaagc aaatcccatc cttcgagtgt cggggcgttt accaggattg
     2701 caagcgttac acttgtgatg ctgtgcgggg gtgaccggcg cgctcacccg cgccgacgag
     2761 gtgatcgaaa cgttctgccg atggtgccgg tgcgagagtc cgctgatggc ggatactgtt
     2821 gcaaaagtca gatttgcgat ccgaagccgg ggatggagac gccggaggcc caattgtgcc
     2881 gtgagtggga gctcgtctac gcggcgcttc gaaatgatgc gtcggtaact gccgagagtg
     2941 aactgaaagg ttttctagct cgtaaaatat ccgggagatg ggtcgctgcc atcagataga
     3001 tcacggtaaa gccaactgag agctagaaaa gcgggcatct tgatttcagt tccgccaagg
     3061 ttcgtgctgc atagcttccg agtgaacgac cgctcctggc gcagagctgc cgttcgcagc
     3121 caaaacagtg ggaaaggttt gggctgttcg ggtggtttt
//
LOCUS       DATMGX010000291         3472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_132974, whole genome shotgun sequence.
ACCESSION   DATMGX010000291 DATMGX010000000
VERSION     DATMGX010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132974"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..565)
                     /locus_tag="VNR65_04710"
     CDS             complement(278..565)
                     /locus_tag="VNR65_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18013.1"
                     /translation="MATYLIIYDTKKEATAYHAHDKQITDAIKSISDVYWNHLDNTYL
                     IVSALSASRIKDKLSKHLTNEDKVLVVKVDAGNAAWSGFPEKASNWLHARA"
     gene            complement(851..2041)
                     /gene="metC"
                     /locus_tag="VNR65_04715"
     CDS             complement(851..2041)
                     /gene="metC"
                     /locus_tag="VNR65_04715"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HWJ18014.1"
                     /translation="MDKDGKDESELATATALTQLGRDPRRYSGFVNAPVFRGSTVLFP
                     DAESLESRDVEYTYGRPSNPTTRALELALAKLEGGQQTFLTPSGLCAVSTTLLAFAQT
                     GGHILVSDSVYMPTRRFANRVLKRLGVDVEYYDPLIGGGIASLLRPETKLVFTESPGS
                     QTFEMQDIPAICKAARGRGVPVAIDNTWATPLYFRAFDHGVNISIQAATKYIVGHADA
                     LIGAITCDASSAKAVASTHESLGLCVNGEDCFLALRGLRTLSVRLERHWKSGLAIAEW
                     LQTRPEVASVVHPALPGHPGHELWRRDFLGASGLFSIYLKPYSNAAVKRLLNALRIFG
                     MGYSWGGFESLIVPFDCKAARTATAWNAQGPALRLHIGLEDVDDLKRDLECGFAALAD
                     DPQL"
     gene            2512..2994
                     /locus_tag="VNR65_04720"
     CDS             2512..2994
                     /locus_tag="VNR65_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18015.1"
                     /translation="MDPDIVRQQEDAERAAAGLPPKKPVNGSADPVQSRHAVSQAQAT
                     LDTPEPIVDTPETIVPLPEEQPIVSARQSFNPNRRALPPIGSGRQPWEEAPPRRRVRG
                     LLRLFLYAIAGAALGGILGFVGVSYFHLFVDQAQLFISSMAGGFALLFGLTHLLHYDA
                     "
     gene            complement(3245..>3472)
                     /locus_tag="VNR65_04725"
     CDS             complement(3245..>3472)
                     /locus_tag="VNR65_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18016.1"
                     /translation="IRRAPVNHFPINARRGDERFTIRRAASAVQHGRARLAKHAIVPS
                     DVGGTVIVARNSPRRVPPNCVATGSDLRTPR"
BASE COUNT          727 a          994 c         1016 g          735 t
ORIGIN      
        1 acggcgaaag ccgctcaacc catctgagac cgcgagcaga ccgctccaca tcgagaaaag
       61 ccgcgctgcc acgcgacggc tgcggaagcg gcaaagatag agaacgcaag ggctggctcc
      121 agaactattg gagcgtcagc gtatctgatt cgtcttgcgt gaacctcgag gggaggtgct
      181 acaagctgtg gacggataaa aaatcacgaa gccgctgaga aacctcccag cggcttttcg
      241 ccccgcggcc cgttaaggac tcgcgaccca aaatcggtta ggcgcgtgcg tgcagccagt
      301 tggacgcctt ctcgggaaag ccactccagg cagcgtttcc ggcatccacc ttgacgacca
      361 ggaccttatc ttcattggtg agatgcttgc tcagcttatc cttaattcgg gacgctgaaa
      421 gagccgagac aatgagataa gtgttgtcga gatggttcca gtagacgtcg gaaatggatt
      481 tgatggcatc ggttatttgc ttatcatgcg catgataggc ggtggcttct ttttttgtgt
      541 cgtagatgat cagataagta gccattgcgg ctcccccttg gttcatcaat actcagatac
      601 agtgtaggct cgacgcgagt cacgccacgg tagaattctg ttcgtgaatt cacatccgcc
      661 ccaccggcgg ctttccagga acgcaaaagc tcagctccgc tttggcctta agtctccact
      721 cgaccaggca taagctttgc gttgacgcca gtgtatcaac acgaaagaga tattcactcg
      781 gtttcgcgtg tttcattttg cggccattcg tcgccagccg caggggaaga caagtaaaac
      841 aaaagaatac ctagagttgt ggatcgtccg caagggccgc gaaaccgcat tcaagatcgc
      901 gtttcagatc gtcgacatct tcgaggccga tatgcagacg gagcgcaggg ccttgcgcgt
      961 tccaagcggt ggctgttctt gctgccttgc agtcgaaagg cacgatcagg ctttcaaagc
     1021 cgccccagga atagcccatg ccgaaaatgc ggagcgcatt cagcaggcgc ttcaccgctg
     1081 cgttcgagta aggcttcaga tagatcgaga aaagaccgct cgcgcccaga aaatcacggc
     1141 gccacagctc atggcccgga tggccgggga gagcagggtg caccaccgac gcaacttcgg
     1201 ggcgcgtttg cagccattcg gcaatggcga ggcccgactt ccaatgccgt tccagtcgta
     1261 ctgagagggt gcggagcccg cgaagagcaa gaaagcagtc ttcgccattt acgcagaggc
     1321 caagactttc atgggtggac gcaaccgcct ttgccgacga cgcatcgcaa gtgatcgcgc
     1381 cgatcagcgc gtctgcatgg ccgacaatat atttcgtggc tgcctggata gaaatgttaa
     1441 cgccgtggtc gaaggcgcgg aaataaagcg gtgtggccca tgtgttgtcg atggcgacgg
     1501 ggacgccacg ccctcgcgcc gctttgcaga tcgcggggat atcctgcatc tcgaaggtct
     1561 gggatcccgg agactccgtg aagacgagtt tcgtctcggg cctcaagagg ctcgcaatcc
     1621 caccgccaat caagggatcg taatactcga cgtcgacgcc aagccgcttg aggacacggt
     1681 ttgcgaagcg ccgcgtcggc atgtaaacgc tatcgctgac aaggatgtgt ccgccggttt
     1741 gcgcgaaagc cagaagtgtc gtcgagacgg cgcacagtcc cgacggggtc agaaacgtct
     1801 gctgcccccc ttccagtttc gccagcgcaa gttccagcgc ccgtgtggtc ggattggacg
     1861 ggcggccata ggtgtattcc acgtctctgg actcaaggct ttcggcgtcc gggaagagaa
     1921 ccgtcgaccc tctgaagacg ggcgcgttga cgaaacctga atagcggcgc gggtcgcggc
     1981 cgagctgagt caaggccgtc gcggtcgcca gctcgctttc atccttgccg tccttgtcca
     2041 tgttacctcc aggttgcagc gggttccttc gccttttcgt gacgcatcgt caaggcttca
     2101 ccgggcggga aaatctgtct ggctacgaat gcttggcagg gacgagtccg ctgccttgaa
     2161 cgaggtgatt cggtcttctt cgcaaatatg cgggggttgg aatgtgtcgc gacctcgcgt
     2221 ttaccattcg tccacagcct ctttgcatga gccggcgtca catcaggggc attcatcatc
     2281 cttttgaagg acacgctccg ctggtgttta gttactgtgc tgtctcgttt tcttccgaag
     2341 attgaggagc gtcactcgca tgtccttgca ttggagcttt cttttcatct aagctacatt
     2401 tttgccgcac ttctttttgc ttcttctggt gtgatactga ttgagtgtta ataccccgct
     2461 tgaacgcagc aaggagcccg gttcataaga ccgggtcaga gggacgatca catggatcca
     2521 gacatagtgc gtcagcagga agatgccgag cgagcagccg ccgggcttcc gccaaagaaa
     2581 ccggtaaatg ggtctgcgga cccagttcag tcacgacacg ctgtttccca ggcgcaggca
     2641 acattggata cgccagagcc catagtggat acgccagaga ccatagttcc tctgcctgaa
     2701 gaacaaccga tcgtctcggc aaggcagtca ttcaacccca accggcgcgc gcttccgccc
     2761 ataggctcag gccgccagcc atgggaagaa gctccgccgc gccggcgggt acgcggcttg
     2821 ctgcgccttt tcctttatgc catcgcgggc gcagcgttgg gcggaattct cggcttcgtc
     2881 ggggttagct acttccacct gttcgtggac caggcccagt tattcatctc aagcatggct
     2941 ggaggcttcg cgctcctttt cgggctgacc cacctcctgc attacgacgc ctgatttgca
     3001 cgctgggggc cgccctagac caactcgcct ttaatcggaa atagattgaa ggcggaaaag
     3061 ttgatcgatt ctagcgtggc gtgcgcgaaa tcggaatcag ttccttgtcg tcccggaacg
     3121 acgcgtgcgt cgtatccggg accgcaaaag gcttttcgat ttgtaacgac cccggatatg
     3181 cgccgaggcg cattccgggg cgacgatcat ctggacgccc gcgtccgatt aaacgcccga
     3241 tgctttagcg cggcgtgcgc aaatcggaac cggttgcgac gcaattcggc ggaacgcgcc
     3301 gaggcgaatt ccgggcgacg atcacggtgc cccccacgtc cgatggaacg atggcatgct
     3361 tcgccaatct ggcgcgacca tgttggacgg cgctcgccgc acgccttatg gtaaaccgtt
     3421 cgtcgccgcg ccgagcgtta ataggaaagt ggttaacagg agcgcgccgg at
//
LOCUS       DATMGX010000292         4087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_56117, whole genome shotgun sequence.
ACCESSION   DATMGX010000292 DATMGX010000000
VERSION     DATMGX010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56117"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VNR65_04730"
     CDS             complement(<1..1178)
                     /locus_tag="VNR65_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18017.1"
                     /translation="MRSAAQRALKWRTGIAPAFAAFIAAFVIALTVMATPSGAVEPIV
                     IGPDDEKIDITLLGEFYERRGDKLSVETASSGDGIAGRMTVSAKTPGTNPSWVVFALS
                     NPTEKSIVRFLTAQRYDIVGSRVTKPDLDAPRISNVTPSLGFRPERNEDYEHLDIYRL
                     SIEPGTTVTFIVELASASVPRLYLTSPANFGKRMRESALFRGILLGITGIIAIFLTAI
                     FAANHKLVFPATALVAWAALSYLCVDFVFWDNLFQLPSEDNGTYRAAAEAFLAASLVI
                     FLYTFLNLRLWHSWISLTFLTWIAAQLGLIFFALIDAQLTSGFARLSFVPIAAIGSAV
                     IGFLALRGQERALALVPSWMLFMVWLFAAAVTITGKVSGDIVVSALSAGLVVFLMLIG
                     V"
     gene            complement(1224..1793)
                     /locus_tag="VNR65_04735"
     CDS             complement(1224..1793)
                     /locus_tag="VNR65_04735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWJ18018.1"
                     /translation="MFFSDSRPYVEGDGVYLREPQMSDYKAWAALRAESRAFLEPWEP
                     RWARDELTRDTFRRRIRYYYRDARQDLGYAFFLFRTSDNALIGGITLSNIRRGVSQSC
                     SLGYWIGEPYARQGQMTKGVRAAIWFVFDVLRLHRLEAACLPENKASAALLNRTGFQF
                     EGLARRYLKINGIWQDHWLFALLSDDPAR"
     gene            complement(1818..3098)
                     /locus_tag="VNR65_04740"
     CDS             complement(1818..3098)
                     /locus_tag="VNR65_04740"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWJ18019.1"
                     /translation="MTVQVSQLSNGMRIVTHRMAGLETATVGVFVAAGARAETDSEHG
                     VAHFLEHMAFKGTPTRTPMQIAEEIEGAGGALNAVTSSEATNYYARVLKDDVELGLNL
                     IGDLLLNPAFSEEEMDREREVILQEIAATQDSPDDIVFDLALAEAYPGQALGRSILGT
                     ERTISRQSAADLKRFRNENYSATRMILSAAGAVDHDALHRLADQIFTGLPSEAPRQIK
                     KAKFEGGNTVSDKRFEQCHVLFAFEGYPNGHEASFAGRIFAGIAGGGMASRLFQEVRE
                     KRGLCYDIHAFDWGYADSGVIGLHAATSPQQLKELCNLSLGIFADLAEKGPTERELAR
                     AKAQIKAGLFMSLESCESRAGQLAWDLLVFGRPISNEELIERVDGITCEHVQAIGRGI
                     RAKPDMVSAAVGARGSAAAVADSAEAFLAGALTA"
     gene            complement(3115..>4087)
                     /gene="thrC"
                     /locus_tag="VNR65_04745"
     CDS             complement(3115..>4087)
                     /gene="thrC"
                     /locus_tag="VNR65_04745"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWJ18020.1"
                     /translation="TGAAAVEAFRGRKDIELFILFPNGRISDVQRKQMTAAQEDNVHA
                     IAIEGTFDDAQAIVKALFGDVDFRTRHALSAINSINWVRIIAQAVYYYTAHAKLGPDA
                     RPSFTVPTGNFGDIFAGFVASKMGLPMGRLVIATNENDILARTLETGRYEPREVMPTD
                     SPSMDIQVSSNFERLLFEESGRDSSFVIHAMANLREKGAFDIPAPALARMRERFSAYR
                     TNREEAASAMDRLFEQTGMLIDPHTAVGLAAAQKEQERRPGPMVVLGTAHPAKFPEAV
                     NRATGRTAEIPERLQARLCGRERFKLLPNSAKAVADTIETQASARHS"
BASE COUNT          822 a         1240 c         1240 g          785 t
ORIGIN      
        1 acgccgatca gcataagaaa gaccacgaga ccggccgaca gcgctgacac aacaatatcg
       61 cctgagacct tgcccgttat cgtgacggcc gccgcgaaca gccaaaccat gaagagcatc
      121 caggacggca ccagcgcgag ggcgcgttcc tgtccgcgca aagccagaaa gccgatcacg
      181 gcgctcccga tggctgcgat cgggacgaag gagagccgtg cgaaaccaga tgtaagctga
      241 gcgtcaataa gggcgaagaa gatcaagccg agttgcgcgg ctatccacgt caaaaacgtg
      301 agcgagatcc aggaatgcca gagccttaaa ttcaggaagg tgtaaaggaa gatgacaagg
      361 cttgccgcca ggaaggcctc ggcggccgct cgataggtgc cgttgtcctc ggatgggagc
      421 tgaaaaagat tatcccagaa gacgaaatcg acgcacaaat acgaaagcgc agcccaggcg
      481 acgagcgctg tcgccggaaa gacaagcttg tggttggccg cgaaaattgc cgtcaggaag
      541 atcgcgataa tacccgtaat gccaagcaga atgccgcgaa acagagctga ctcccgcatc
      601 cgcttcccga agtttgctgg cgaggtcaga taaagccttg gaaccgacgc cgaggcgagt
      661 tccacgatga atgtgacggt ggtgccgggt tcgatcgaaa ggcggtaaat atcgagatgc
      721 tcgtaatctt cgtttcgttc agggcggaag cctagcgacg gcgtaacgtt cgagattcgc
      781 ggggcatcga ggtccggctt ggtgacgcgc gatccgacaa tgtcgtatcg ctgggccgtc
      841 agaaagcgaa cgatggactt ctcagtcggg ttgctgagcg cgaaaaccac ccagctcggg
      901 ttcgtgcccg gcgtttttgc cgagacggtc atgcgtccgg cgatgccgtc gccactcgac
      961 gcggtttcga cgctcagctt atcgccacgg cgctcataga attcgccgag aagggtgata
     1021 tcgattttct cgtcgtcggg gccgatgacg attggctcca cagccccgga cggcgtcgcc
     1081 ataaccgtca gggctatgac aaatgcggct atgaaagccg caaatgccgg cgcaatgcct
     1141 gtccgccact tgagcgccct ctgagccgcc gaccgcacag ttgggctttt tccccgcccc
     1201 ttcatcgccg gttgccgcta gggtcaacgc gcagggtcat cactgagaag agcaaaaagc
     1261 caatggtctt gccatatgcc attaattttt aggtaccgcc gggccaagcc ctcgaattga
     1321 aaccccgtcc ggttcagaag cgccgccgac gctttgttct ctggcaggca cgccgcctcc
     1381 aaccgatgaa gacgcaaaac atcgaagaca aaccaaattg ccgcccgcac acctttggtc
     1441 atttggcctt gccgggcgta tggctcgcca atccaatatc ccaaggagca gctttggctc
     1501 actccacgcc gtatattgct taaagttatt cccccgatta aggcgttatc agaggttctg
     1561 aacagaaaaa aggcgtaacc gaggtcctgg cgggcatcgc ggtagtagta gcggatacgg
     1621 cggcgaaaag tgtcgcgggt cagttcgtcc ctcgcccagc gtggttccca cggttcgaga
     1681 aaggcgcggc tttccgcgcg tagtgcggcc caggccttgt agtccgacat ttgaggctcg
     1741 cgcagataga cgccgtcgcc ctccacgtag ggcctgctgt cggaaaagaa catgcgggcg
     1801 ccgagcatgc cggccggtca cgcggtaaga gcaccggcca ggaacgcttc ggcagaatca
     1861 gccaccgctg ccgccgatcc ccgggcgccc acggctgccg aaaccatatc cggcttcgcg
     1921 cggatgccgc gaccaatcgc ttgaacgtgc tcgcaggtaa tgccatcgac cctttcgatc
     1981 agttcctcgt tcgagatcgg cctgccgaac accaagagat cccacgcaag ctgccccgct
     2041 cttgactcgc agctttcgag gctcatgaag agcccggcct tgatctgagc cttggcgcgc
     2101 gcgagttctc gctccgtcgg gcctttctct gcgagatccg cgaatatgcc cagcgagaga
     2161 ttgcacaatt ccttcagttg ctgcgggctc gtcgccgcgt gaagcccgat cacgcccgaa
     2221 tcggcatagc cccaatcgaa ggcatggatg tcgtagcaga ggccgcgctt ctcgcgcacc
     2281 tcctggaaaa gccgcgaagc catgccgccg cccgcgattc ctgcaaaaat gcggcccgca
     2341 aaggaagcct cgtggccatt cgggtagccc tcgaaggcaa agagcacgtg gcactgctcg
     2401 aagcgcttgt ccgagaccgt attgccgccc tcgaacttgg ccttttttat ctggcgcggc
     2461 gcttcggaag ggaggccggt gaagatttga tccgcaagcc gatgcagcgc atcgtggtcg
     2521 acggctcccg ccgcgctgag gatcattcga gtggccgaat agttctcgtt gcgaaagcgc
     2581 ttgaggtcgg cggcggattg ccgggagatg gtgcgctccg taccgaggat cgagcggccc
     2641 agcgcctggc cgggataggc ttcggcaaga gcgagatcga acacgatgtc gtccgggctg
     2701 tcctgcgtcg cggcgatttc ctgcaggatc acctcccgct cccggtccat ctcctcctcg
     2761 ctgaaggctg gattgagaag aagatcgccg atcaggttca ggccgagttc cacgtcgtct
     2821 ttcaaaacgc gggcgtaata gttggtggcc tcggatgagg tgacggcgtt cagtgcgcca
     2881 ccggcgccct cgatttcctc ggcgatctgc atcggcgtgc gggtcggcgt gcccttgaag
     2941 gccatatgtt cgagaaaatg ggcgacgcca tgttcgctgt cggtttcggc tcgcgcgccg
     3001 gcagccacga aaaccccgac ggttgctgtt tcgagtcccg ccatacggtg cgttacgatc
     3061 cgcatcccat tgctgagctg gctcacttga actgtcatgg tttgacctcg aatgtcatga
     3121 atgccgcgcg ctggcctgtg tctctatagt gtcggcgact gccttcgcgg agttcggcaa
     3181 aagcttgaac ctctcccggc cgcaaaggcg ggcctgaaga cgttcgggaa tctcggctgt
     3241 tcggcctgtg gcccggttaa cagcttcggg aaacttggcc ggatgcgcgg tgccaagcac
     3301 caccatcggt ccgggcctgc gttcctgctc tttttgagcc gcagcgagcc ccacggctgt
     3361 atggggatcg atgagcatgc ccgtctgctc gaagagcctg tccattgcgg acgcggcttc
     3421 ctcgcgattg gtccggtaag ccgaaaagcg ctcgcgcatc cgtgcaaggg ccggggccgg
     3481 aatgtcgaac gcgcccttct cgcgcaaatt tgccatcgcg tgaatcacaa agctcgagtc
     3541 gcggccgctt tcctcgaaca gaaggcgctc gaaattgctt gaaacctgaa tgtccatgct
     3601 cgggctgtcc gtgggcatca cctcgcgcgg ctcgtagcgt cctgtttcca gcgtgcgagc
     3661 aaggatatca ttctcgttcg ttgcgatcac aagccttccc atcggcagcc ccatcttcga
     3721 ggctacgaag cccgcgaaga tgtcgccgaa atttccggtc ggaaccgtga agctcggccg
     3781 cgcatcgggt ccaagctttg cgtgcgcggt atagtagtac accgcttgcg cgatgatgcg
     3841 cacccagttg atggagttga tcgcagaaag cgcgtggcgc gtgcggaagt ccacatcgcc
     3901 gaacagcgcc ttcacgattg cctgcgcatc gtcgaaggtg ccttcgatgg cgatggcgtg
     3961 gacgttgtct tcctgcgccg ccgtcatctg cttgcgctgc acgtcgctga tgcgcccgtt
     4021 cgggaagagg atgaaaagct cgatgtcctt gcgcccgcga aaggcttcca ctgcagccgc
     4081 gccggtg
//
LOCUS       DATMGX010000293         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_234187, whole genome shotgun sequence.
ACCESSION   DATMGX010000293 DATMGX010000000
VERSION     DATMGX010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234187"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VNR65_04750"
     CDS             complement(<1..1193)
                     /locus_tag="VNR65_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HWJ18021.1"
                     /translation="MNTPASKKSGWGRRLQQSEFFLDKVGSFAASHIRMVRDTSKVIR
                     EPADTDSYLEQLNPMIVAMWHGQFLLLPLIKPKTVAVHNMVAKHADGEMIGRALLHFD
                     MGLIRGAGAGESGKDRGGAGALRAALKLLKSNVTVALTTDVPPGPARVAGMGIVTLAR
                     MSGRPIVPLAIATDRFITLPTWSRFTINLPFSNLAMVAGEPIHVPRDADQSTMEEFRQ
                     QVEAAMNAATARAYELAGSDYRKTAPKVAKQHSSGMLLSVYRGATALARPAAMTILRN
                     RAARGKEVPERLLERLGIAGVPRPDGRLFWFHAASVGETNSILPLMHELKRLHPALNL
                     LLTTITATSAKIAAARLPEGAIHQFLPLDSPRFCRRFIEHWRPDLGLLAESEIWPNLI
                     VEASNG"
     gene            complement(1251..2078)
                     /locus_tag="VNR65_04755"
     CDS             complement(1251..2078)
                     /locus_tag="VNR65_04755"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HWJ18022.1"
                     /translation="MQNDYAQDFDLIRDAAQEAGKLALSYWGRSIEKQRKADGSEVTE
                     ADHAVDALLAKLLKDARPTYGWLSEESAEHNERLRGRRVWIVDPIDGTRAFIQGRDDW
                     VIALALVVDHVPVLAAVVNPVRGQTFEARAGAGTFLNGRKIHASEQSSLSGARLTVND
                     SLLKANRWRRPWPPVTPVVANSIIYRMALVASGTADASFATKPKWEWDVAAGALLVSE
                     AGGIVTDPCGFPLKFNSREAKVPGYVAAAPNLHHILAERMSDALDGAPVAQGHKTTT"
     gene            complement(2091..>2668)
                     /locus_tag="VNR65_04760"
     CDS             complement(2091..>2668)
                     /locus_tag="VNR65_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWJ18023.1"
                     /translation="VGLAVLIQFNLFAICLGFASVGIVLIYPLMKRVTFWPQAFLGLA
                     FSWGALMGWAAIFGSVALAPVILYGSAVAWTIGYDTIYAHQDKEDDEVIGLKSTALRF
                     GERTPHWLSLFYGLTWGGLILSGWLVGAGWLFFAGMLLAGAHLIWQIATLDIDDSQNC
                     LDRFRSNHGFGAIVFFALVLDIIPVALSSLG"
BASE COUNT          557 a          820 c          805 g          486 t
ORIGIN      
        1 ccgttgctcg cctcgacaat caggttcggc cagatttcgg attccgcaag aaggccgagg
       61 tcggggcgcc agtgctcgat gaaacggcgg cagaagcgcg ggctgtccag ggggaggaac
      121 tgatggatcg cgccctcagg gagccgggcg gcggctattt tcgcggatgt cgcagtgatt
      181 gtcgtgagca gcagattgag cgctggatga aggcgcttca actcgtgcat gaggggcagg
      241 atcgagtttg tctccccgac gcttgccgca tgaaaccaga aaagccgccc atccgggcgg
      301 ggcacgcctg caatccccaa acgctccaga aggcgctccg gcacttcctt gccgcgcgcc
      361 gccctgtttc tgaggatggt catggccgcg gggcgcgcta atgccgtcgc tccgcgatag
      421 acggaaagca gcatgccgga gctgtgctgt ttcgcgacct ttggcgcggt cttgcgatag
      481 tcgctcccgg caagctcata agcgcgcgcc gttgccgcgt tcatggccgc ttccacctgc
      541 tggcggaact cctccatcgt gctttgatcc gcatcgcggg gcacatggat cggctcgcct
      601 gccaccatcg caaggttgga gaacggcaga ttgatggtga agcggctcca ggtcggcagc
      661 gtgataaagc ggtctgttgc gatggcgagc ggcacaatgg gccgccccga catccgtgcc
      721 agcgtcacga tccccatacc tgcaacgcgc gcgggtccgg gcgggacatc cgtggtcaag
      781 gcgaccgtca cattggactt gagcaacttt agtgctgccc ggagcgcgcc cgcgccgcca
      841 cggtccttgc ccgactcacc agcgcccgcg cctcggatca ggcccatgtc gaaatgcaga
      901 agcgcccggc cgatcatctc gccatcggca tgtttggcga ccatgttgtg cacagcgacc
      961 gtcttgggct tgatcagcgg caagagcagg aattggccgt gccacatggc cacaatcatc
     1021 ggattgagct gttcgagata ggaatcggta tcggcaggtt cacggatgac cttggaggtg
     1081 tcgcgcacca tgcggatatg actagccgca aagctgccta ccttatccag aaaaaactcc
     1141 gattgctgca ggcggcggcc ccaaccgctt ttcttggaag ctggtgtgtt catcgagcgc
     1201 cgaattttct cgtcaccaag tgcaaagcga gaccctgttg agggatcgcg tcaggttgtg
     1261 gtcttgtgcc cttgcgcaac aggtgcgccg tccaatgcat cactcatacg ctcggccaaa
     1321 atgtgatgca aatttggggc ggccgcaaca tatccgggaa cttttgcctc tcttgagttg
     1381 aacttgagtg gaaatccaca gggatctgtg actatccccc ccgcctcgga cacgagaagc
     1441 gccccggcag cgacgtccca ctcccatttt ggttttgtcg cgaaggaagc gtccgccgtg
     1501 cctgaggcga cgagggccat gcgatagatg atagaattgg caacgaccgg cgtcacgggc
     1561 ggccagggcc gccgccagcg gttggctttc aggagcgagt cgttaacggt gagccgagca
     1621 ccgctcagcg aactttgctc gctcgcatgg atctttcgcc cgttgagaaa cgtacccgcc
     1681 ccggcgcggg cttcaaatgt ctgaccccgc acgggattga cgactgcggc gagaaccgga
     1741 acgtgatcga cgaccaaggc cagcgcgatc acccaatcgt cccgcccctg gatgaacgct
     1801 cgggtgccgt cgatgggatc gacgatccag acgcggcggc ctcgaagacg ctcattatgc
     1861 tcggcgcttt cttcggaaag ccagccatag gtgggacgcg catccttaag cagcttcgcc
     1921 agcagcgcgt ccacagcatg gtcggcctcc gtcacctccg agccatcggc cttgcgctgc
     1981 ttttcaattg atcggcccca atagctcaga gcgagctttc cggcctcctg ggcggcatcg
     2041 cgaataagat cgaagtcctg cgcgtaatca ttttgcatac cagctacatg tcagccgagc
     2101 gacgagagcg caaccggtat gatatcaaga accaatgcaa aaaacacaat cgcgccaaat
     2161 ccatggttgc tgcgaaaccg atcgaggcag ttctggctgt cgtcgatgtc gagagtggcg
     2221 atctgccaga tgagatgcgc acccgccagc agcatcccgg cgaaaaacaa ccaacccgcc
     2281 ccgacgagcc atcccgagag gatgaggcca ccccaggtca ggccataaaa cagactcagc
     2341 cagtgaggcg tccgttcgcc gaacctcaga gccgtggatt tcagtccgat aacctcatcg
     2401 tcttccttgt cttgatgcgc gtagatcgtg tcgtatccga ttgtccaggc gacagcagac
     2461 ccgtacagaa ttaccggcgc cagagccacg gacccgaaaa tcgcggccca acccatgaga
     2521 gcgccccatg agaaggcgag gccgagaaaa gcctgcggcc agaaggtgac acgcttcatc
     2581 aagggatata tcaacacgat gccgaccgaa gcgaagccca ggcagatcgc aaacaggttg
     2641 aattggatga gcacggcgag accgacga
//
LOCUS       DATMGX010000294         2769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_49462, whole genome shotgun sequence.
ACCESSION   DATMGX010000294 DATMGX010000000
VERSION     DATMGX010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49462"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VNR65_04765"
     CDS             complement(<1..1178)
                     /locus_tag="VNR65_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HWJ18024.1"
                     /translation="MPPSEPREAAVSPQRKFTRQEALQFHSEGKPGKLEIRPTKPMAT
                     QRDLSLAYSPGVAVPVEAIAENPRLAYDYTAKGNFVAVISNGTAILGLGNLGALASKP
                     VMEGKAVLFKRFADVDSIDIEVDTQDVDAFVNAVRYLGPSWGGINLEDIKAPDCFIIE
                     SRLRELMDIPVFHDDQHGTAIIATAGLINALHLTGRDIADIKLVCNGAGSAGIACLEL
                     VKAVGVRHENVTLCDTSGVIYRGREKGMNQWKSAHAIDTKARTLAEAIEGADVFFGLS
                     AAGALTKEMVASMAERPIIFAMANPDPEITPEEVAQVRDDAIVATGRSDYPNQVNNVL
                     GFPYIFRGALDVRAKTINMEMKIAAARALAALAREDVPDEVAALYGARPKYGPDYIIP
                     V"
     gene            1502..>2769
                     /locus_tag="VNR65_04770"
     CDS             1502..>2769
                     /locus_tag="VNR65_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWJ18025.1"
                     /translation="MISVSITERPPAAFSNRKSLRTHMCKARSTAKRYLTAAFFAGAA
                     LLPLAGHAEEPQDQAPQASAGSQQIPINSGKAAEAVLSAVNAYRAENKIGELAWNNAL
                     WQTAQHYAEFLAANKASGHGADGKIPQQRAEENGYPCPVAENTYSADPAETTDDEDTA
                     ALKAFDTWKNSPPDNDNLLSPIWTETGIGAAAWNHEGQTYYVFVQLFGHPCCGAKAEP
                     SHLIEPAPAPLIQRPLVDQTPDYVETPPVVVPVCPEWDPDCRGCPAGNPYCHRCPPWD
                     PDCRRCPSWNPYCNKCPPWDPSCRRCPPWNLHCHGCPPWNPNCRQCPPSNPNCRQHVC
                     PNGGIWPRCNPIGHVCPNGGIWPRCNPVSHFCPNGGIWPRCNPIGRVCPNGGIWPRCD
                     TIGHVCQNGGVWPDCNKTGTHLCPNGGVWPR"
BASE COUNT          525 a          873 c          817 g          554 t
ORIGIN      
        1 acgggaatga tgtaatcggg gccgtatttc ggccgcgcgc cgtatagcgc cgcgacctcg
       61 tccggcacgt cctcgcgcgc caaagcggcc agcgcacgcg ccgcggcgat cttcatttcc
      121 atgttgatcg tcttggcgcg cacgtcgagc gcgccgcgga agatgtaggg gaagccgagg
      181 acgttgttga cctggttggg atagtccgag cggcctgtcg cgacgatggc gtcgtcacgg
      241 acctgggcga cctcctccgg cgtgatttcc ggatcggggt ttgccatggc aaagatgata
      301 ggccgctcgg ccatactggc gaccatctcc ttcgtcagcg ctccggcagc agatagtccg
      361 aagaaaacat ctgcgccttc aatggcttcg gcaagcgtcc gcgctttcgt gtcgatcgcg
      421 tgggccgact tccactggtt catgcccttt tcgcggcccc ggtagatgac gccagacgta
      481 tcgcaaagcg tcacattctc gtggcgaacg cccaccgcct tcacgagttc gaggcaggcg
      541 atgccggcag aacccgcgcc gttgcagacg agcttgatgt ccgcaatgtc gcggcccgtc
      601 agatgcaggg cgttgatcag ccccgcggtc gcaatgatgg ccgtgccgtg ctgatcgtca
      661 tggaagacgg ggatgtccat cagctcgcgc agccgactct cgatgatgaa gcagtccggc
      721 gccttgatgt cttccaggtt gatgccgccc catgacggac cgagatagcg cacagcgttc
      781 acgaaggcgt ccacatcctg ggtgtcgacc tcgatatcga tggaatcgac gtcggcgaag
      841 cgcttgaaga gaacggcctt gccttccatc accggcttcg acgccagcgc gcccagattg
      901 ccaagcccga gaattgcggt gccgttcgaa atcacagcga cgaagtttcc cttcgctgtg
      961 tagtcatagg cgagccttgg gttctcggcg atagcctcga cgggaaccgc cacgcccggc
     1021 gaataggcga gactgagatc gcgctgcgtc gccatcggct tcgtcggccg gatttcgagc
     1081 tttccaggct tgccctcgga gtgaaattga agcgcctctt ggcgcgtgaa tttgcgttga
     1141 gggctgacag ccgcttcccg cggttcggag gggggcaatt cggacacggt ctttagcctc
     1201 gcccttgcgg cgggtttaga agggggcatc gcttttcttt gttgaatgag ctgaaatgga
     1261 ttgaatgcgg ctccgcttgg aacggcttgc tgtgacgtta gccccgcaga ttgtaccgca
     1321 caagcgcgca gcagccattc aaggaacctt ttgtactaag gtgtgatgcg ctcgcctatc
     1381 ccgtatcccg gtcaatgcca ttgattttcg cgtgcgtttg aaccttctcg tgcccccgaa
     1441 cgactcagcc taaggtggcg gaacctctct cttgaggccc cgcctttcgc tggaggactc
     1501 aatgatttct gtgtccatta ctgaaagacc gcctgcggct ttttcgaacc gcaaaagcct
     1561 gcgcactcac atgtgcaaag cccgaagcac ggccaaaaga tatctcaccg ctgcgttttt
     1621 cgcgggcgcg gcgctcctgc ctctcgcggg ccacgctgag gaaccgcaag atcaagcgcc
     1681 ccaagcttcg gcgggatcgc agcaaattcc aatcaacagc ggcaaggcgg cggaggctgt
     1741 gttgtcggcc gtaaatgcct accgcgccga gaacaagatc ggtgagcttg cttggaacaa
     1801 tgcgctctgg cagaccgcgc aacactacgc cgagttcttg gccgccaaca aagccagtgg
     1861 tcacggagcc gacggcaaaa tcccccagca gcgagccgaa gagaacggct atccttgccc
     1921 tgtcgccgaa aacacctatt cagccgatcc tgctgagacg acagacgacg aagataccgc
     1981 cgcgctcaaa gccttcgaca cttggaagaa ctctccaccc gacaacgaca atttgctttc
     2041 accgatctgg accgagacag ggatcggagc ggcagcctgg aaccacgaag gccaaaccta
     2101 ttacgttttc gtgcagcttt tcgggcatcc gtgttgcggt gccaaggcgg aaccgtccca
     2161 cttgattgag ccggcgccag caccgcttat ccagcgcccc ctcgtcgacc aaactccaga
     2221 ctatgttgaa acgcctcccg ttgtagtccc tgtttgccca gaatgggatc ccgattgccg
     2281 aggctgtccg gcggggaatc cgtattgtca tcgatgcccg ccttgggatc cggactgccg
     2341 caggtgcccc tcatggaatc cctattgcaa caagtgcccg ccctgggatc cgagctgtcg
     2401 tagatgtccg ccttggaacc tgcattgcca cggctgcccg ccctggaatc cgaactgccg
     2461 ccagtgcccg ccttccaatc ccaattgcag gcagcatgtc tgcccgaacg gcgggatctg
     2521 gccgcgctgc aacccgatcg gtcatgtctg ccccaacggc ggcatctggc cgcgctgtaa
     2581 cccggttagt catttctgcc ccaacggcgg tatctggccg cgttgcaacc cgatcggtcg
     2641 cgtttgcccg aacggcggta tctggccacg ttgcgacacg atcggtcatg tctgccagaa
     2701 tggcggagtt tggccagact gcaataagac cggcacgcac ctgtgcccga acggtggagt
     2761 ctggccccg
//
LOCUS       DATMGX010000295         3836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_94817, whole genome shotgun sequence.
ACCESSION   DATMGX010000295 DATMGX010000000
VERSION     DATMGX010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94817"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /gene="phaR"
                     /locus_tag="VNR65_04775"
     CDS             <1..938
                     /gene="phaR"
                     /locus_tag="VNR65_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HWJ18026.1"
                     /translation="SIFVSFQRLFERKFIRINVPQECQIRCHCHFASRAASAGLFPQW
                     RKREPKFFAGAKKIHCDAWNLHYNRLGKTPLGDKMNKPVEKLSRATVFHEAGQSFMTG
                     AGSKKRDGGDKSKPVIIKKYANRRLYNTETSSYVTLEDLGEMVRSERDFLVYDAKTGE
                     DLTHTVLTQIIVEEEGKSGSNLLPIGFLRQLIRFYGHSIEQLVPKYLEFSLATLTREQ
                     EKYRKQFTDTFGAAAFEAMQEQARQNMVMFERAFAMFAPFSKIEPRTEEEKSAQEKPE
                     PAPPSTSAEIAELKAQLSAMQAQLDRLSRSTTTGE"
     gene            1859..3337
                     /gene="guaB"
                     /locus_tag="VNR65_04780"
     CDS             1859..3337
                     /gene="guaB"
                     /locus_tag="VNR65_04780"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWJ18027.1"
                     /translation="MRQKMGLTFDDVLLQPSHSDVLPSEVDVTTSLTRSIKLNIPIVA
                     AAMDTVTEGPLAIAMAQAGGIGVIHRNMKPEEQAQHIRQVKKFESGMVVNPVTISPHA
                     TLKDALSLMKSFRISGIPVVEGANSNGQGKLVGVLTNRDVRFATNPAQPVHELMTKSN
                     LITVKDNIGRDDAKRLLHRHRIEKLIVVDDDYQCIGLITVKDIEKAEQHPFACKDEQG
                     RLRVAAATSVGDDGFNRSEHLMDAGADLIVVDTAHGHSQRVLDAVKRIKRASNVVQVI
                     AGNVATAEGTLALIDAGADAVKIGIGPGSICTTRIVAGVGVPQLTAVMESAEAAARHG
                     VPVIADGGIKYSGDIAKALASGAACVMVGSLLAGTDESPGEVYLYQGRSYKSYRGMGS
                     LGAMALGSADRYFQSEVADALKLVPEGIEGQVPYKGPVSSVLHQLVGGLRAAMGYTGS
                     HSIAELHEKAEFVRITNAGLRESHTHDVTVTRESPNYPIGNA"
     gene            3347..>3836
                     /locus_tag="VNR65_04785"
     CDS             3347..>3836
                     /locus_tag="VNR65_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18028.1"
                     /translation="MQPGAKLQAAIEVLTEIMERGRAASVVLADWGRAHRFAGSGDRA
                     WIGNLVFDSLRRKQSLSYVMRSETARAIALAALRSNWNVSLQDIASFCSGEGFGPARL
                     TPEEESGLANSDLADAPPWIQGDYPEWLHPSFSEVFGDRAVAEGQALAARAPIDLRVN
                     TLK"
BASE COUNT          856 a         1086 c         1103 g          791 t
ORIGIN      
        1 cgtccatatt cgtttccttt cagcgcttat ttgagcggaa gtttatcagg attaatgttc
       61 cgcaggaatg tcaaattcgt tgccattgcc attttgcgtc cagagcagcc tcagccgggt
      121 tattcccaca gtggaggaag cgcgagccta aattcttcgc aggtgcaaaa aaaatccact
      181 gcgacgcgtg gaacttgcac tataataggc taggcaagac accattgggc gataaaatga
      241 ataaacctgt cgaaaagtta tcgcgggcaa ccgtttttca cgaggcggga caatcgttta
      301 tgacaggcgc ggggagcaaa aagagggacg gcggggataa gtccaaaccc gttatcatta
      361 agaagtacgc taatcgaagg ctttataaca cagagacaag cagctacgtc actctcgaag
      421 atcttggcga gatggttcga tcagagcgag attttctcgt ctacgatgct aagacgggtg
      481 aagatctcac gcatacagta ttaacccaga ttattgtgga agaggaaggc aagagcggtt
      541 ccaacctcct tcccattggt ttcttacgcc agctgatccg gttctacggt cactctattg
      601 agcagcttgt gccaaagtat ctcgagttca gtcttgcaac tctgacgcgc gaacaagaga
      661 agtatcggaa gcaatttacc gacaccttcg gcgctgcagc attcgaggcc atgcaagaac
      721 aagcgcggca gaatatggtg atgttcgagc gcgcgtttgc gatgttcgcg ccattctcca
      781 agatcgagcc ccgcacagag gaagagaaaa gcgcgcagga gaagcccgag cccgcacccc
      841 catcaacctc agccgagatt gcagagctca aagcgcaact ctccgcgatg caagcccagc
      901 tggaccgcct ttcccggtcc acaacgactg gtgaatagaa ttcaaaaata agctgaggct
      961 gcagcaccgg gttaatctac cccgccctgc ccatgcttcg atgtcgctag ctccaaggcc
     1021 gcaaccgtcg ccgagggtca ggcggctgtc tcatgccgat gttggctttc gagttgaata
     1081 gtctgcgccc tgttcctcga tgacagcgaa ttctcgacga tggtgagagc atcatccaac
     1141 gatggaaacc tctcacccag ggaaacttac aatgctccag cgacaagacg cgttgcgcta
     1201 tagctctcga aactaccgcc cctttttagg aaccctcgaa atcattgccc tccccgcact
     1261 ggccggatcg accttgctgt ttcttacatc caacaaggtg gaaatgcctg gtgatttgga
     1321 ctacacaacg tcactctacg ggccgtagcc gaagctgaca ggcttcggtt tcaacaacgg
     1381 aattacgccc ggaggccggt gtgacagaat gggcttgttc gtgcggcaag ctcacggcgt
     1441 aggctggcat gtcgcgcatt aatatgcggc cgctcatgca gcggcctcaa ttggagaggg
     1501 gtatttattt acctaagcta ggagcccgga atcttgccca gtctgtgcgt gttcagccct
     1561 tgcctgtacg cctggcttaa tgtcctccat gggaacaagg ccggaaccag ccactgacaa
     1621 atgcaggcac tttcggtccc tgatcaggga ttttggtgcc ctttcgttcc cttccagagc
     1681 tttttgggat tgccccttta gtctgacaac atattgattt ataaagattt ttttaccctt
     1741 acatacgggg atttgattcg cgtaccgttg ccagatcgag acgggggtgc taaaccccag
     1801 caagaattcc aaacagatcg acgcggcttg gcgtgcaaag ggttcgcaaa tggccattat
     1861 ccggcagaag atggggctta ccttcgacga tgtgctgttg cagccgagcc attcagacgt
     1921 gttgccgtcc gaagttgacg ttacgaccag cctgacgcgg agcataaagc tcaacattcc
     1981 gatcgtcgcg gcggccatgg atacggtcac tgaaggcccg ctcgccatcg cgatggctca
     2041 ggccggtggc atcggcgtca tccatcgcaa tatgaagcct gaagaacagg cccagcacat
     2101 ccggcaggtg aagaagttcg aatccggcat ggtggtcaat ccggtcacca tctcacctca
     2161 cgcgaccttg aaagacgcgt tgagcttgat gaagagcttt cggatctcgg gcatcccggt
     2221 tgtcgagggt gccaatagca acggccaggg caagctcgtc ggcgttctta cgaaccgcga
     2281 tgtgcgtttc gctacgaacc ccgcacagcc ggttcacgag ctgatgacca aaagcaatct
     2341 catcacggtg aaggacaata tcggacggga tgacgccaag cggctcttac acaggcatcg
     2401 catcgagaag ctgatcgtcg ttgacgatga ctaccagtgc atcggcctga tcaccgtgaa
     2461 ggacatcgag aaggccgagc agcatccctt tgcttgtaag gacgagcagg ggcggcttcg
     2521 cgtggccgcg gcgacctccg ttggcgatga tggcttcaat cgcagcgaac atctcatgga
     2581 cgcaggcgca gacctgatcg tcgtcgatac cgcgcatggc cactcgcagc gcgtcctcga
     2641 cgccgtaaag cgcatcaagc gcgcctccaa tgttgtccag gtgattgccg gcaacgttgc
     2701 gaccgccgag gggacgttag ccctcatcga tgcgggcgcg gacgccgtga agataggcat
     2761 aggcccgggc tcaatctgca caacgcgtat cgtcgcgggc gttggtgtgc ctcagctgac
     2821 cgccgtcatg gaatctgcag aggctgcggc gcggcacggc gtgccggtca tcgccgatgg
     2881 cggcattaaa tattcgggcg acatcgcgaa ggcgctggcg agcggcgcgg cttgcgtgat
     2941 ggtcggctcg ctgctggcgg gcaccgacga gagccccggc gaggtttatc tgtatcaagg
     3001 ccgctcatac aaatcctatc gaggcatggg ctcgctcggc gccatggcgc tcggatctgc
     3061 agaccgctac ttccagagcg aggttgcaga cgccctgaag ctcgtgccag agggcattga
     3121 agggcaggtt ccctacaaag ggccggtgtc aagcgtgctg caccaacttg tgggcggctt
     3181 gcgcgcggca atgggctaca caggctcgca ctccattgcc gaactgcatg agaaagcgga
     3241 atttgtcagg atcaccaacg cgggcctccg ggaaagccac acgcacgacg tcacggtcac
     3301 gcgagagtcg ccgaactatc caatcggcaa cgcttaagcc gcggcgatgc agcccggcgc
     3361 aaagctacag gccgcgatcg aggtcttgac cgaaatcatg gagcgtgggc gggccgcttc
     3421 cgtcgtgctt gccgattggg gcagggctca ccgctttgca ggctccgggg accgcgcctg
     3481 gatcggcaat ctcgtcttcg acagcttgcg gcgaaaacaa tccttatctt atgtgatgcg
     3541 ctcggaaacc gcgcgtgcca tagcgctcgc cgcgcttcgc agcaactgga atgtttccct
     3601 tcaggacatc gccagctttt gcagcggtga ggggtttggc ccagcgcgtc tcacgcccga
     3661 ggaggagagc ggcctcgcaa atagcgacct ggcggatgcg cccccttgga tacaaggtga
     3721 ctaccccgaa tggcttcatc cctcattctc tgaggttttc ggagatcgcg ccgttgcgga
     3781 ggggcaggct ttggctgcac gcgctcccat agatttgcgc gtcaatacgc tgaaag
//
LOCUS       DATMGX010000296         2324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_347590, whole genome shotgun sequence.
ACCESSION   DATMGX010000296 DATMGX010000000
VERSION     DATMGX010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347590"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..731)
                     /gene="rpiA"
                     /locus_tag="VNR65_04790"
     CDS             complement(3..731)
                     /gene="rpiA"
                     /locus_tag="VNR65_04790"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HWJ18029.1"
                     /translation="MTQETQAYKRQAAAQALQLVEADMVLGLGSGSTALAFVELLAER
                     IKEENLKITCIATSEQTRAFAEQHGIKIADFNTISDLDLTVDGADEIDHQLNLLKGGG
                     GALLREKIVAMASDHVCIIADNSKLVRQLGAFPLPVEIADFGLAATIAMIETAAEDAG
                     CKGEVKLRRTGTGDLYRTDGGHLIADCAFGAIPDVETLADLLDIVPGVMEHGLFLGIA
                     DDVFLAGPEGAKHLQAGRDDDDED"
     gene            complement(728..2176)
                     /locus_tag="VNR65_04795"
     CDS             complement(728..2176)
                     /locus_tag="VNR65_04795"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWJ18030.1"
                     /translation="MEQTPQALIRQQCLINGQWQGEPTTPVTDPATGGTIARVPNLGA
                     SETRVAIDAASRAFPAWSGLLAKERAAILRRWYELQLAHADDIAQIMTAEQGKPIAES
                     RGEVSYGASFVEFFAEEAKRIYGETIPTHRKGGRIIVHKQPVGVVGAITPWNFPFAMI
                     TRKVAPALAVGCTVVVKPAPDTPLTALALGELALRAGFPAGVLNIVTGDAEAIGKELT
                     SNPLVRAITFTGSTRVGKLLMQQSAATVKKVSLELGGNAPFIVFDDADLDMAIEGAIA
                     SKFRNMGQTCVCANRILVQDGVYETFAQKLADRVSKMKVGPGSDEGVVQGPLINEAGL
                     LKVEAHVADALSKGAKAIVGGKRHALGRTFFEPTVLIDVNADMRLATEETFGPVAPLF
                     RFKHEDEAIRFANATPYGLAAYFYSRDVARVFRVTEMLEFGIVGVNDGIISTEVAPFG
                     GVKESGLGREGSRHGVEEFLEMKYMCLGGLSD"
BASE COUNT          466 a          723 c          684 g          451 t
ORIGIN      
        1 atctagtctt cgtcgtcgtc gtcgcgccca gcctggagat gcttcgcgcc ctcaggaccg
       61 gcgaggaaca cgtcatcggc tatgccgagg aagagcccat gttccatgac tcccggaacg
      121 atatcgagca aatccgccaa agtttccaca tccggtatcg cgccaaacgc gcaatcggcg
      181 atcagatgcc cgccatcggt gcggtaaagg tcgccagtgc cggtgcggcg aagcttgact
      241 tcgcctttgc aaccggcgtc ctcggccgcc gtttcgatca tggcgatcgt ggccgcgagg
      301 ccgaaatccg cgatctcgac cggcaacgga aatgccccaa gctgccgaac cagcttcgag
      361 ttgtccgcaa tgatgcagac atggtcggac gccatggcga caattttctc tcgcaggaga
      421 gcgccgccgc cacctttgag caaattcagc tgatgatcga tttcgtccgc gccgtccacg
      481 gtgaggtcca aatcagagat cgtgttgaaa tcggcgatct tgatcccgtg ctgctccgcg
      541 aatgcgcgcg tctgctccga agtcgcgatg caggtaatct tgagattctc ctccttgatg
      601 cgctcggcca aaagctcaac gaatgcaagc gctgtcgagc cggagccaag gccgaggacc
      661 atgtccgctt caacgagttg aagtgcttgc gctgcagcct ggcgcttgta agcctgcgtt
      721 tcttgggtca atccgaaagt ccccctaggc acatgtattt catttcgaga aactcctcga
      781 ccccgtgcct tgagccttcg cggcccagcc cggattcctt cacgccgccg aaaggcgcaa
      841 cctcggtcga gataataccg tcattcacgc cgacaatgcc aaattcgagc atctcagtca
      901 cgcggaacac gcgcgcgaca tcccgcgaat agaaataggc ggcgaggcca taaggagtag
      961 cgttcgcaaa gcggatggct tcgtcctcat gcttgaagcg gaagagcggc gcgacgggcc
     1021 cgaaagtctc ctcagtcgca agccgcatgt cggcattcac gtcgatcaaa accgtcggct
     1081 cgaagaaggt tcggccgagc gcatggcgct tcccgccgac gatagccttg gcgcctttcg
     1141 agagcgcatc cgcgacatgc gcctcaacct tcaggagccc cgcttcgttg atgagcggcc
     1201 cctgcaccac gccttcgtca gagcccggcc cgactttcat cttcgaaacg cggtcggcca
     1261 gcttctgcgc gaaagtctca taaacgccgt cctggaccag gatgcgattg gcgcacacac
     1321 acgtctgccc catgttgcgg aatttggatg cgattgcgcc ttcgatagcc atgtcgagat
     1381 cggcgtcgtc aaatacgatg aacggcgcgt tgccgccaag ctccagcgaa accttcttca
     1441 ccgtcgctgc ggactgctgc atcaagagct ttccgacccg cgtggaaccg gtgaaggtga
     1501 tggcgcgcac aagcgggttt gatgtcagct ctttcccgat ggcctctgca tcgccggtga
     1561 cgatgttcag gacaccagcg ggaaaaccag ctcgcagcgc aagctcgccc aacgccagcg
     1621 ccgtaagtgg cgtatcaggc gctggcttca cgaccaccgt gcaaccgacc gccagcgccg
     1681 gagcaacctt gcgcgtgatc atcgcgaaag gaaaattcca gggcgtgatc gcgcccacaa
     1741 cgcctaccgg ctgcttgtgg acgatgatgc ggccaccctt tctgtgggtc gggattgtct
     1801 cgccgtaaat gcgcttcgct tcctcggcga agaactcgac gaaagatgcg ccgtaggaga
     1861 cttcgccgcg cgactcggcg atcggcttcc cctgctccgc cgtcatgatc tgcgcaatgt
     1921 cgtcggcgtg ggcgagttgc agctcatacc agcgccgcag gattgccgcg cgttccttcg
     1981 caaggaggcc gctccaggcc gggaatgcgc gggaggcggc atcgatggca acacgcgttt
     2041 ccgatgcgcc gagattcgga acgcgggcga tggtgcctcc cgttgccgga tcggtgacgg
     2101 gtgttgtggg ctcgccctgc cattggccgt tgatcagaca ttgctgacgg atcaaggcct
     2161 gaggggtctg ctccattgtc ggcggcatcc ttaatcttgg cggaggtggc atcatgccgc
     2221 gacgcggccg aaactacaag cgcggctgga ttacagcgcg ctgtttttta aatagggcaa
     2281 acaatcgata aatgaaaagc atatcggcga aaataggtag cggt
//
LOCUS       DATMGX010000297         2315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_260303, whole genome shotgun sequence.
ACCESSION   DATMGX010000297 DATMGX010000000
VERSION     DATMGX010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260303"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..715
                     /locus_tag="VNR65_04800"
     CDS             245..715
                     /locus_tag="VNR65_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18031.1"
                     /translation="MPIARYMVWVGTSLLALLFGANWLLPEPLPESVHDAIDRPVIRI
                     ASVQQSPERVVIDTNQPTIVPPPALFERSGSGNASPLQSYASVNPSPVVVDSARKRPK
                     AIKRREAKVASYEPAQSNTLAATRGGSTTIVPSARLSFADIISGRLVRNLFNLH"
     gene            complement(1016..1828)
                     /locus_tag="VNR65_04805"
     CDS             complement(1016..1828)
                     /locus_tag="VNR65_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ18032.1"
                     /translation="MNDTIVSSYVTGSAQMQGDLWSARARDYAELQESHFLPLYESVQ
                     RRPELTKSRSSLDVGCGPGLAAQVFSQTIANVAGIDASAAFIDIARQRLPGRNLRVGE
                     METLPHADGSFDVVTGFNAFQYAASPVNALREARRVTQPDGVVVIATWGLPEDCEAAG
                     HLKALGALMPPPPPGAPGPFALSDESKLKALVFEAGLTPIAVVDVECPWIYPNLDIAL
                     RAMLSAGPAERAIRNSGMDRAREAVANSITPYLKPSGQYHLSNKFRYLIARA"
     gene            complement(2001..>2315)
                     /locus_tag="VNR65_04810"
     CDS             complement(2001..>2315)
                     /locus_tag="VNR65_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ18033.1"
                     /translation="RNHILLEHQPAWKQFLAEIARFVHGQGRADAFEETAFAELTQRE
                     REVLELIARGLDNTAIADQLSVSPKTVRNHINGIFDKLNIPNRAQAIVRAREAGMGTR
                     TG"
BASE COUNT          481 a          658 c          688 g          488 t
ORIGIN      
        1 tgattattcc gaccgagcag ttcaagcgcc gtgccgcatc gtctggcgca aggacaggcg
       61 gatcggagtg acgttcgatt aggaacgtcg gccgcctgac gcattatcac aatagatcag
      121 atcaaggctc cgagtggcaa ctttaagact accaacttga attcgagcag taaccacgtt
      181 ccataacgat ctttcagtaa tttgcggcgt ttgctctcgt cattatttat ggcgagacat
      241 tcctatgcca atcgcgcgat acatggtctg ggttggcaca tcgcttctgg cactgctgtt
      301 cggggcaaat tggctgcttc ccgaacctct gccggagtca gtgcatgacg cgatcgacag
      361 gccggttatt cggatagctt cggttcagca gtcgcccgaa cgggtggtca ttgacaccaa
      421 tcagcccacg atagtaccgc caccggcact gttcgaacgc tcgggatcag gcaatgcctc
      481 gcccctgcaa tcgtacgctt ctgtgaaccc atcaccggtg gtggtcgact cagccaggaa
      541 gcggccgaag gcaatcaaac ggcgtgaggc caaagttgct tcctacgaac ctgcccagtc
      601 gaacacgctt gccgccacgc gcggcggctc gacgacaatc gtaccgtcag ccaggctatc
      661 gttcgcggat atcatttctg gacggctggt gagaaacctt ttcaacctgc attgacctcg
      721 cggtagcgcg aactgttttg ggcaatcgaa atggcttttt gaatcgacct gacggcatcg
      781 gcgggatgaa ggacaatatt ccctggtagc tcgcctccgg tgcgcgtgaa cgagttccga
      841 gctggcacgt ttgagacatg ccgactgacc ttgggaatgt acgctgatcg gggtagaccg
      901 gaaatcgttg gtagccggtc aaaacgacgc gaatgacccg ttacggatat tgagcatcga
      961 tcaacgcgtc caaggtcgta ccggccaatt ccgtagcgaa aaaccatcgg tccgatcacg
     1021 cgcgggcgat gaggtagcgg aacttgttgc tcagatggta ctgacctgaa ggcttcaggt
     1081 agggcgtaat cgagttcgct accgcctcac gtgcgcggtc catgccggaa tttcggatgg
     1141 ctcgttcagc agggccggca gacagcattg cacgaagcgc aatgtccagg ttcggataga
     1201 tccagggaca ttcaacatcg accactgcta tcggcgtaag tccggcctcg aataccagcg
     1261 ctttcaattt ggattcgtcg gacaatgcga aagggccagg agctccgggc ggcggcggtg
     1321 gcatcaacgc accgagggct ttgagatgcc cggctgcctc acagtcctcc ggcaagcccc
     1381 aggttgcgat caccacaacg ccatccggct gtgttacgcg gcgtgcctcg cgcagagcgt
     1441 tgacaggcga cgcggcatat tgaaacgcgt tgaagccagt cacgacatcg aaacttccat
     1501 ccgcgtgcgg cagcgtttcc atttcgccga cacgcaaatt gcgcccaggc aggcgctgac
     1561 gcgctatgtc gatgaacgcg gccgatgcgt cgatgccggc gacgttggca attgtctgtg
     1621 aaaatacctg cgcggcaagg ccaggaccgc aaccgacatc gaggctcgac cgcgatttcg
     1681 taagttccgg ccggcgctgc acgctctcgt acaacggaag gaagtggctt tcctgcaatt
     1741 cggcataatc gcgcgctcga gcactccaca ggtcgccttg catttgcgcg gagccggtga
     1801 cgtaggacga tacaatggtg tcattcatcg ttgtctcctt gggcgttgaa ataccgccgg
     1861 gtgcttggca aaaatgattg gtcgtcgggg gagaaatgtg tttgggatgg cccaagccgt
     1921 catgagaccc tcggcccagg cctcatccca acgtgttgga atgttctgaa atgctgggcc
     1981 acgagaccca gatcgggcgc ctagcccgtt cgcgttccca tgccagcctc gcgcgcgcga
     2041 acgatggctt gggcccggtt ggggatgttc agcttatcga agatgccatt gatgtgattg
     2101 cgaacggttt ttgggcttac cgatagttgg tctgcgatcg ctgtattgtc gaggccgcga
     2161 gcgatcagtt cgaggacctc acgctcgcgt tgcgtgagtt cggcaaacgc cgtctcctcg
     2221 aaagcgtcgg cccggccttg accgtgcacg aagcgggcga tctcggccag gaactgcttc
     2281 caagccggct gatgctccaa caggatgtga ttgcg
//
LOCUS       DATMGX010000298         2217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_151936, whole genome shotgun sequence.
ACCESSION   DATMGX010000298 DATMGX010000000
VERSION     DATMGX010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151936"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..767)
                     /locus_tag="VNR65_04815"
     CDS             complement(252..767)
                     /locus_tag="VNR65_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ18034.1"
                     /translation="MIPPNHRETIASMKAEIPRLRRFARYMTRDADYSDDLVQECLTR
                     AIANIGSWQPGTNLRAWLFVILKNVFRNDKRRAQHDMAYRSGLERDAPLVSPAQQHNH
                     LVLSEVQEAFLQLTEDHREVLMLIAVEGLRYEEAATILNISVGTVKSRLSRARTALRA
                     LIDIPPASGRE"
     gene            978..1778
                     /locus_tag="VNR65_04820"
     CDS             978..1778
                     /locus_tag="VNR65_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18035.1"
                     /translation="MEDASPRRLDEEPVGTSDNRVDPSDLRPSDLPDPSALPEIRIYS
                     RSSIFFWWPLWVTGYILAAITYLGGQRIALSNGSSILVHPNTGLGISFMVILTLIMML
                     TNVRLRGIYSVVGLLFMAFVTVLFAWLGWWDRIFSLIPQLTVYMNFGFYMTFSTLLLA
                     IWALRFFFFDRLIFWRVRPGQLTEEHWIGGGEQSYDTRGMLFEQRGNDFFRYGLGFGA
                     SDLLLITTGARKQEIDIPNVLFANQKIHNMQRLIAIKPEEVLAKTARY"
     gene            complement(1820..>2217)
                     /locus_tag="VNR65_04825"
     CDS             complement(1820..>2217)
                     /locus_tag="VNR65_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWJ18036.1"
                     /translation="RLREIDYGLWEAKSTEEIHAVGGADELKAWDERGIWPTSPGWSP
                     PHEGISANVAQIAQNLSTLLGRDDAALLVTSNGILKFFLKLVPGAFEVLAANRELKVA
                     TGNCCALHHEESTWSVAFWNRPPRHFGLD"
BASE COUNT          469 a          608 c          632 g          508 t
ORIGIN      
        1 tcatcatcga aacccggccg ggcggtcgcc acgctcaaga cgacaacgac aaaccagaac
       61 ggggaaatcg tgcaggtgat gacttcgaag aacattgtct tcaagcacgg ccacgtgcca
      121 ggcgaaacgc tgagtgcggg gttagaaggc taagctaatc tggccggcgg attatcatcg
      181 tggccgctgc tgcgaattcg tcatccttcc gggacgacgg cggcgccatt ggcgcgtccg
      241 gcaaactctg cttactcccg cccggacgcg ggcggaatgt cgatgagggc ccgcagcgct
      301 gtccgggccc gcgaaagcct cgatttcacc gtgcctacgg aaatgttgag aatcgtcgcc
      361 gcttcctcat accgcagacc ttccacggca atcagcatca gcacctcgcg gtgatcttcc
      421 gtgagctgaa gaaaggcctc ctggacctcc gacagcacaa ggtgattgtg ctgctgggcg
      481 ggagagacaa ggggcgcatc tctttccaag ccgctgcggt aggccatgtc atgctgagcg
      541 cggcgcttat cgttccgaaa aacgttcttg agaatgacga aaagccaagc ccgtaagttt
      601 gtgcccggtt gccacgaccc gatattggcg attgcacgcg tgaggcattc ctggacaaga
      661 tcgtccgaat aatccgcgtc gcgggtcatg tagcgcgcaa agcgccgaag gcgagggatt
      721 tccgccttca tggaagcaat ggtttcgcgg tggtttggcg ggatcaactc tcacgtcctg
      781 tcaggcctgc gagatataga ggcatattta taagaaagta agggcttctt gcgtcatcca
      841 aatggatggt cgcgaagaag ttatttcgca ttcgagcaat tattgcgttg ctccggactg
      901 cggaacttcg agcaaaagcg tcgtgttagc ggtgtacaga acgacgccga cttggcggcc
      961 ttttctagga ggacgatatg gaggatgcct cgccgcgcag gctggacgaa gagccggtcg
     1021 gaaccagcga caatcgggtc gatccatccg atctgcggcc ttccgacttg cccgatccca
     1081 gcgcgttgcc ggagattcgt atttatagcc ggtctagtat cttcttttgg tggccgctgt
     1141 gggtgaccgg ctacatcctt gcagccatca cataccttgg cgggcaacgt atcgccttgt
     1201 ccaacgggtc atcaatcctt gtccacccca atacaggcct cggaataagc tttatggtaa
     1261 tcctgacgct tattatgatg ttaacgaatg tcaggttgcg cggcatatat tcggttgtcg
     1321 gcctgctttt catggcgttc gtgaccgtct tatttgcctg gctcggctgg tgggatcgca
     1381 tcttttccct catcccgcaa cttaccgtct acatgaactt cggcttctac atgacgttct
     1441 cgactcttct cttggccatt tgggcgttga gatttttctt cttcgatcgc cttatatttt
     1501 ggcgggtacg ccctggacaa ctcacagaag aacattggat cggcggcggg gagcagagct
     1561 acgatacaag gggtatgctg ttcgagcagc gcggaaacga ttttttccgc tatggtctcg
     1621 ggtttggcgc gtcggatctc ctattgataa caacgggcgc gagaaagcag gaaattgaca
     1681 ttccgaatgt tcttttcgcg aaccaaaaga ttcacaacat gcagcggctc attgccatca
     1741 agccggaaga ggtcttggca aagacggctc gctactgacc cagtttgcaa gggggcgccg
     1801 gaaacaaaac gcctcttcgc taatcaagtc cgaagtgccg gggcgggcgg ttccaaaacg
     1861 cgacgctcca agtgctctcc tcatggtgga gagcacaaca atttccggtg gcgaccttta
     1921 gttctctgtt cgccgccagt acttcgaagg cgccggggac tagtttaagg aagaatttga
     1981 ggatgccatt gctggtgacg agcagggcag catcatccct gcccagaagg gttgagagat
     2041 tctgcgcgat ctgcgccacg tttgcggaaa tgccctcatg aggcggagac caacccggcg
     2101 atgtcggcca tatgcctctt tcatcccagg ctttcagctc atcggctccc ccgactgcat
     2161 ggatttcctc ggtggacttg gcttcccaga gcccgtaatc gatttcacga agacgct
//
LOCUS       DATMGX010000299         3150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_420547, whole genome shotgun sequence.
ACCESSION   DATMGX010000299 DATMGX010000000
VERSION     DATMGX010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420547"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VNR65_04830"
     CDS             <1..334
                     /locus_tag="VNR65_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18037.1"
                     /translation="VVVNYIREAQIAAGVPRDHIYDFTLYILAGMLVVGFFANLAVHH
                     VSEKRYMSAAEVEAMTARDDEPAYHGSRVIGRGTFLTPGAIFAWLLVGIPLAWGVWVT
                     VQKAVVLF"
     gene            complement(408..926)
                     /locus_tag="VNR65_04835"
     CDS             complement(408..926)
                     /locus_tag="VNR65_04835"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWJ18038.1"
                     /translation="MADATERANSVFVTGLKNAHALENQALELMNRQVERIENYPDIK
                     AKLEQHIEETHNQIQRLERILGSLGESRSILKDNALSLTGNMAAIAHALTQDEILKNS
                     IANFAFENFEIASYKSLIQLAGDTGHHEAEQPLQETLREEEAMASWIDGHLSDVTHTY
                     LERYVADQKAGL"
     gene            complement(997..2049)
                     /locus_tag="VNR65_04840"
     CDS             complement(997..2049)
                     /locus_tag="VNR65_04840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ18039.1"
                     /translation="MSNRIEGFAHLAGQTLRTVSFTALARYVSRNAARRDGDGTRVEI
                     TKPVPGTLTLLQNVMKLQAKDARNVRDGLYPIPIEESTSFASRIESVRLMLKDQAQLQ
                     ERRAEKRSTDVAELPEAAGMPDYYAQNFHYQTGGWLTEESARLYDIQVETLFLGAAGA
                     MRRQAIPPIAEFIRGRDQRQLRLLDVACGSGRFLAQLSQAFPALPMVGSDLSQAYLDE
                     AARFLQGRRNVTFQQANAETLPCESASFDIVTCVYLYHELPNEVRRRVTSEIARVLKP
                     GGLFAFVDSLQWDDVEGYGGLLEAFPQRYHEPYYLDYLGDTLDGPDGLFAKSGLQPSS
                     SFPALASKIVVCRKGA"
     gene            2169..2885
                     /locus_tag="VNR65_04845"
     CDS             2169..2885
                     /locus_tag="VNR65_04845"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HWJ18040.1"
                     /translation="MTRYHHIVVLTGAGLSAESGLHTFRDKDGIWARYDYREVATPEG
                     YHRNPMLVLNFYNERRRQNADVQPNAAHDALARLEADHPGRVLVVTQNIDPLHEMAGS
                     KNLIHMHGEILKALCGACGRRHSIGHNDLSLDSVCPVCAEAGYMRPDVVWFGEMPYNM
                     ERIYEALGSCDLFISIGTSGTVYPAAGFVLEARRGGAHTVELNLEPSEGISGFHEAIH
                     GRATEVVPAYVDRLLSAARG"
     gene            complement(2940..>3150)
                     /locus_tag="VNR65_04850"
     CDS             complement(2940..>3150)
                     /locus_tag="VNR65_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18041.1"
                     /translation="ILLEIHDMKEDLEAPQALQKSALAEFNSLKLRPIPKLSSYRDIA
                     TALLRAEVVMRSAALQAVEYGETKH"
BASE COUNT          627 a          915 c          926 g          682 t
ORIGIN      
        1 cgtagtcgtc aactacatcc gcgaagcaca gatagctgct ggcgtgccgc gcgatcatat
       61 ctacgacttc accctgtata tattggccgg gatgcttgtc gtcggcttct tcgccaatct
      121 cgccgtccac catgtgtccg agaagcgcta tatgagcgcc gcggaagtgg aggccatgac
      181 ggcgagagat gatgagcctg cctatcacgg ctcccgcgtg atcggccggg gcacgttcct
      241 gacgcctggc gcaattttcg cttggcttct cgtcggcatc ccgctggcat ggggcgtttg
      301 ggtcacggtg cagaaggccg tcgttctttt ttaggctgag gaaatgtaag aatgcgaaag
      361 gcggcgctga tggcgccgcc ttttttttga gagggctcag tcgagtatca aaggcccgcc
      421 ttctggtcgg cgacgtaccg ctccagatag gtgtgggtca cgtccgacaa atggccatcg
      481 atccagctcg ccatcgcttc ctcttcgcgc agcgtctctt gcaaaggctg ctcggcttcg
      541 tgatggccgg tatcgccggc aagctgaatg agcgacttat atgaggcgat ctcgaaattc
      601 tcgaaggcga agttggcgat cgagttcttc aggatttcgt cctgcgtgag cgcatgcgcg
      661 atcgcggcca tatttccggt cagcgataaa gcgttatcct tgaggataga ccggctttcg
      721 ccgagcgacc cgagaatccg ttccagccgc tggatctggt tatgggtctc ctcaatatgc
      781 tgctcgagct tggccttgat gtcggggtaa ttctcgatcc gctcgacctg cctgttcatc
      841 aactcaagcg cctgattctc gagagcatgg gcgttcttaa ggcccgtgac gaagacggag
      901 ttcgcgcgtt ctgtggcatc tgccatgggt aaatctctcc ttgttgtccc atgacaatgc
      961 aaggcattag ccggtgttcc gaattgcagc ggcgtcttac gcacctttcc ggcagacaac
     1021 gatcttcgac gccaacgccg gaaaactgct tgaaggctgc aatcccgatt ttgcgaaaag
     1081 cccatcgggg ccgtcgagcg tgtcccccag gtaatcgaga tagtacggct cgtggtagcg
     1141 ctgcgggaag gcttcgagca ggccgccata gccttcaaca tcatcccatt gcagtgagtc
     1201 tacgaaagcg aaaaggccgc ccggcttgag cacgcgcgcg atttccgacg tcacccggcg
     1261 gcgaacctcg ttgggcaact cgtgataaag atagacgcag gtcacgatgt cgaagcttgc
     1321 gctttcgcag ggaagcgtct cggcgttagc ctgctggaac gtcacgttgc ggcggccttg
     1381 aaggaagcgt gcggcctcgt cgagataggc ttggctcaga tcgctgccga ccatcggcag
     1441 ggcggggaag gcctgggaga gttgcgcaag gaaacggccg ctcccgcacg ccacatccaa
     1501 aagccgaagc tgccgctgat cgcggcctcg gataaactcc gcaatcggcg gaatagcctg
     1561 gcggcgcatt gcgcccgcag ccccgagaaa cagagtctca acctggatgt cgtaaagcct
     1621 tgccgattcc tccgtaagcc agcccccggt ttgatagtga aaattctgcg cgtaataatc
     1681 cggcatgcca gctgcttccg gcagctccgc cacatccgtg gaccgttttt cggcacgtcg
     1741 ctcctgcaat tgagcctggt ctttcagcat cagccgtacg gattcgatgc ggctcgcaaa
     1801 cgaagtgctc tcttcgatgg gaatgggata aagaccatcg cggacgttgc gtgcatcttt
     1861 cgcctgaagc ttcataacgt tctggaggag cgtgagcgtg cctggcacgg gcttcgttat
     1921 ttcaacgcgc gtcccatcac cgtcacggcg tgcggcgttc cggcttacat agcgggcaag
     1981 cgcggtgaag ctgacagtgc gcaaggtttg cccggcgagg tgcgcaaagc cttcgattcg
     2041 atttgacatg atcttactct ctccggacac tcagacgaca attacgaagc gggataaact
     2101 ccgggtcacc taccccaagg aggtaaatcg cccgcccaga aaaattcaag tggagagagt
     2161 gctccctgat gactcgctac catcacattg ttgttttgac aggcgctgga ctttccgcgg
     2221 agtccggcct tcatacgttc cgcgacaagg acgggatctg ggcgcgctac gattatcgcg
     2281 aggtcgcaac gccagaaggc tatcaccgca atcccatgct cgtcctgaat ttttacaacg
     2341 agcgtcgaag gcagaatgcg gatgtgcagc ccaatgccgc gcacgatgct ctggctcgcc
     2401 tcgaggccga tcatccgggc cgggttctgg tcgtgacgca gaatatcgat ccgctgcatg
     2461 aaatggcggg gtcgaaaaac ctcatccaca tgcatggcga gatcctgaaa gcgctctgcg
     2521 gcgcttgcgg gcggcgccac tcaatcgggc acaacgatct ttcgttggac tcggtctgcc
     2581 cggtttgcgc ggaggcgggc tatatgcgcc ccgacgtggt gtggttcggc gaaatgcctt
     2641 acaacatgga gcgcatctac gaggctctgg gcagctgcga tcttttcatc tcaataggga
     2701 caagcggcac ggtttacccc gccgccggat tcgtgcttga ggctcggcga gggggcgcgc
     2761 ataccgttga gctcaatctc gaaccttccg aaggcatcag cggcttccac gaggcgatcc
     2821 acggccgcgc aaccgaggtc gtccccgctt atgtggaccg gctcctgagc gccgctcgtg
     2881 gctagcacag catgaccgga atcgacaggc agtaacggca gctggcctat atcgcatctc
     2941 taatgcttgg tctcgccata ttcaacggct tgaagcgccg ctgacctcat aacgacttcc
     3001 gccctgagaa gtgctgtggc gatatcgcga taagagctta gcttcgggat tggccgcaat
     3061 ttcaaagaat tgaactccgc cagggcactt ttctggaggg cttgcggagc ttccaaatcc
     3121 tccttcatgt catgtatctc cagaaggatc
//
LOCUS       DATMGX010000300         7165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_111444, whole genome shotgun sequence.
ACCESSION   DATMGX010000300 DATMGX010000000
VERSION     DATMGX010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111444"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VNR65_04855"
     CDS             <1..953
                     /locus_tag="VNR65_04855"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWJ18042.1"
                     /translation="GNTSLLSSSPTFTAQVGGAPVVSTIVDSEPKPWTKSTSDTISVT
                     ATSTNSTVSSVEIYDNGKDAGAATLTNGTWVRTVTGLTDGVHSFSAVATDALGKSSTS
                     LATVVDQVDTTGPTIGSPSQNASGAWTKSNSDTITVAASDGGSGIASVHIYDGQSDLG
                     AATLTNGSYVLTANGLSDGAHVFTAKATDNAGNTTTAAGSVTDLVDHSGAPTLGAISE
                     TTLGKNGEYGQFKFDVSNLVGGQVGPNIGHVTYWTDQSATATQAPTSGWHWANLSLDS
                     KGMGTETLTLNDTGHIGDYLHVQAVDTLGNASAIGTKKIV"
     gene            1074..3392
                     /locus_tag="VNR65_04860"
     CDS             1074..3392
                     /locus_tag="VNR65_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HWJ18043.1"
                     /translation="MLKRVVWDPQSLFTRYAQAITGKLRRGDFKDTIITMLKSRLGWD
                     LSPDPAPAAQLSAALALSDDQVDSGLLALAAIAGYYRIAADWRHLSNELALRGIAAGS
                     NEIIRGAKLIGLKARIIENPSSSRLAAAPVPSIVRLKKGGYCVLAGKTSEGLFRFVDP
                     VTRSARELPLDELVEEIEPVLILVVRNLIGAGIDPRTFGFVWFAPSLWRYRKPLAHVL
                     IASFFVQIFALIAPLFFQVAIDKVLTHRGYSTLFVLIAGLVILGLFDVALQYLRTYAL
                     THTTNRIDVELGQRLFRHLARLPVSYFETRPTGQTVARMRELESIRSFLTGQGLFSAL
                     DLFFAFVFIAVLFAYSWKLTLIVLVTIPLYLIIAGIARPLLRERLNEKFNRGAESQQF
                     LVESIVGMQTIKAAAVEPIVAKQWEEKLAAYVRSAFNTTMLGAKGQNAIQYVSKVSNA
                     ALLLFGAKAVIDGELSVGELVAFNMIAGQVAQPILRLSQIWQDFQQVQVSVERLGDIL
                     NSPVEPTPATRAQLPPPRGKIEFRHVCFRYRPDGPQILKQISFAISPGTVVGIVGPSG
                     SGKSTLTKLLQRFYIPDEGQVLLDDADLSHADPSWLRSHIGVVLQENLLFNRTIHDNI
                     ALSNPAMAREQVMTIARWAGADEFIGKLPQGYDTMVEERGANLSGGQRQRIAIARALA
                     TNPPILIFDEATSALDYESERIIQDNMRQIVKNRTVFIIAHRLAAVRDCDLIIGMTEG
                     RIVEAGSHDDLIKKPHGLYARLWSMQSERAEA"
     gene            3389..4912
                     /locus_tag="VNR65_04865"
     CDS             3389..4912
                     /locus_tag="VNR65_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HWJ18044.1"
                     /translation="MSTVKTIAKAPPSNAVQKSKLHPEDFEFLPADLEILETPPSPVR
                     MALILIICAFVATALIWSWIGHIDIVAVAQGKIQPTGRVKVIQPLETGKVAAIMVENG
                     RRVKAGDLMIEMDIGDARAEEDATKSAYEALRAEARRRRTAIDAAQTHLLPQARHQSW
                     NAETMESATQRRLMSIPKIDWDAGTPEQIRQRENQVLAGDLEQLKATISDFEAQIRQK
                     EIEHDRLDEAIAMQDRLIATLKERVDMRAVLAKSGSGSNANLIDAKETMLYHLSQQAN
                     QKVQRDATIANLDVLAGGRDKAFSNFIAENAQKLAEAQKQADDWREKLAKAKFKSERM
                     SLKSPIDGVVYGLSVTTIGQVVGGGEELMRIVPEDAMLEIECYLANKDIGFVKPGQNA
                     VVKIESFPFTRYGTLSATVTRVASDAIPEPDASQMEGNSARQPKDKGFAGAQRTQNLV
                     FPVTLTPARKAMIVDGLTVPLTPGMAVNVEIATGNRRILEYIFSPLVETASQAGKER"
     gene            5209..5559
                     /locus_tag="VNR65_04870"
     CDS             5209..5559
                     /locus_tag="VNR65_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18045.1"
                     /translation="MACANENAPPVNLPAQIGERIAQCWHAPQTDPPQVIEVTVRLSF
                     SRTGTLIGEPRVVYVRAAKQAGLREKVVNSVIAAIKACTPLPFTPALGAAIAGRIFAI
                     RLNSLPLTGRQRFT"
     gene            5963..6268
                     /locus_tag="VNR65_04875"
     CDS             5963..6268
                     /locus_tag="VNR65_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18046.1"
                     /translation="MAIRLAFAHKFGRLPRGDDPVFFDPDADRPYSLPSGGTQRLLLE
                     AMLENGTAPHIVYAFCRTGFAVSDQNRTALPPDRLSRWDTAINEYLAFGAKAKHSQH"
     gene            6768..7163
                     /locus_tag="VNR65_04880"
     CDS             6768..7163
                     /locus_tag="VNR65_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18047.1"
                     /translation="MTSSASVTYLTRQFDDFLFARIDEGSNPTPLSVLSMLARLDIDP
                     WEEAAKLARLPRAAAARRLVDFIAATPGAPSAYLNAKTVSDRLLNLLPSPALPRQEQA
                     TRALKKVPFVWPAVIVVAILAISLIVMRL"
BASE COUNT         1529 a         2064 c         2051 g         1521 t
ORIGIN      
        1 caggcaatac gtccttgttg tcctcttcac caaccttcac cgcccaggtc ggcggtgcgc
       61 cggtcgtcag caccatcgtt gactccgaac cgaaaccttg gaccaagtct acatcggaca
      121 cgattagcgt gaccgccacc agcaccaaca gcacggtgtc gtcggtggag atctacgaca
      181 atgggaagga tgccggcgcc gcgacgctga ccaacggcac ctgggtgcgc accgtaaccg
      241 gccttacgga cggcgtacat agcttcagcg ccgtagccac cgacgccctg ggcaaatcgt
      301 ccacgtcctt ggcaactgtc gtggaccagg tcgacactac gggaccgacc atcgggagcc
      361 caagccagaa cgcttcgggc gcctggacca agtccaactc cgacacgatt accgtagcgg
      421 ccagcgacgg cggctccggt atcgcctcgg tgcatatcta cgacggccag agcgatctgg
      481 gcgccgcgac gctgaccaac ggcagctatg tcttgactgc aaacggtctc tccgacggtg
      541 cgcacgtctt caccgcgaag gcgacggaca atgccggtaa cacgaccact gcggccggca
      601 gcgtcaccga tctggtcgat cacagtggag ctccgacgct tggcgcgatc agcgagacga
      661 ccctcggtaa aaacggagag tacggacagt tcaagttcga tgtgtcgaac ctggtcggtg
      721 gccaggttgg accgaacatc ggtcacgtca cctactggac cgatcagtca gcgaccgcaa
      781 cccaggctcc cacctctggg tggcattggg ccaacctttc gttggacagc aagggtatgg
      841 gcacggagac acttaccctc aacgacaccg gtcacattgg cgactacctc catgtccagg
      901 ccgtcgacac actggggaac gcttctgcga taggcacgaa gaagatcgtc tgagcgatag
      961 ctcctcaaca cgattatggg gccgccgcac gcggcggccc cattagccag ctccctgtat
     1021 ttagttttta gagtaatcgc tcagtaaacc atgtaaggat tttggtaacg caaatgctta
     1081 agcgtgttgt ttgggacccg caatccctgt tcacccgcta cgcccaggcc ataaccggaa
     1141 aactgcgtcg cggagatttc aaagacacca tcatcactat gctgaagtcc cggcttggct
     1201 gggacttgtc gcctgacccg gcgccagcgg cgcagctgtc cgcagctctt gcgctctcgg
     1261 atgaccaagt ggatagcggg ctgttggctc tggctgcgat tgctggctat taccgcatcg
     1321 ctgcggattg gcggcatctt tcgaatgagt tggcgctgag gggaatcgcc gccggctcta
     1381 acgagattat ccgcggcgcg aaattaatag ggctcaaggc tcgcattatc gaaaatccat
     1441 cgtccagccg tctggctgca gctccggttc catcaatcgt gcgcctcaag aaaggcggct
     1501 actgcgttct cgccggaaaa acgagcgagg ggctctttcg cttcgtcgat cccgtcacgc
     1561 ggagcgctcg cgaattgccg ctggatgagc tcgtcgagga aatcgaaccc gtcctgatcc
     1621 tggttgtccg aaatctaatc ggcgcaggca tcgatccgcg caccttcggc tttgtctggt
     1681 tcgcgccttc gctgtggcgc taccgcaagc cgctcgccca tgtgctgatc gcctcgttct
     1741 tcgtgcagat tttcgcgctc atcgcgcccc tatttttcca ggtggcgatc gataaggtcc
     1801 tgacccatcg cggctactcg accctgttcg tgttgatcgc cggtctggtg atattgggcc
     1861 tcttcgatgt ggcgcttcaa tatttgcgga cctatgcgct gacccacacc acaaatcgca
     1921 tcgacgtcga actcggtcag cggctgtttc gtcatctcgc gcggttgccg gtttcctact
     1981 tcgagacgcg gcccacgggg caaaccgtgg cgcgcatgcg agagttggaa tccattcgct
     2041 ccttcctgac tggccaaggt ctcttctcag cgctcgacct gttcttcgct ttcgtcttta
     2101 tcgccgtcct gtttgcatac tcttggaagc tgacgctcat cgtcctggtg acgatccctc
     2161 tctacttgat catcgcaggc atcgcgcggc ctttgctgcg ggaacggctg aacgaaaaat
     2221 tcaaccgcgg tgcggaaagt cagcagttcc ttgtcgaatc catcgttggg atgcagacca
     2281 tcaaggccgc ggcggttgag cccattgtgg ccaagcaatg ggaggagaag ctggcggcct
     2341 atgtgcgctc cgctttcaac accaccatgc ttggagccaa agggcagaac gcgatccaat
     2401 atgtcagcaa ggtttccaat gccgctctcc ttttgtttgg cgccaaggct gtcatagacg
     2461 gcgaactctc ggtcggcgaa cttgtcgcct tcaatatgat agcgggtcag gtggctcagc
     2521 cgattttgcg gttatcgcag atttggcagg atttccagca ggtgcaggtg tcggtcgagc
     2581 ggctcggcga catcttgaat tcgcccgtcg aaccaacccc ggctacgcgc gcgcagcttc
     2641 cgccgccgcg tggaaagatc gagtttcggc acgtttgttt tcgctaccgc ccagacggtc
     2701 cacagatcct caaacagatt tcgttcgcta tcagccccgg tacagtcgtc ggaattgttg
     2761 ggccttcggg atccggcaaa tcgaccctga ccaagttgct gcaacgcttc tatattccgg
     2821 acgagggcca ggtgctgctg gacgatgccg atctgtccca cgccgatccg agctggctgc
     2881 gcagccatat cggcgtcgtt ctgcaggaaa atctgctgtt caaccgtacc atccacgaca
     2941 acatcgcttt gtctaatcct gcgatggcgc gggaacaggt catgacaatc gcgcggtggg
     3001 ctggcgccga cgagttcatt ggcaagctgc cccagggcta cgacacgatg gtcgaagaac
     3061 gcggcgccaa tctatccggc ggtcaacgcc agcgcattgc gatcgcccgt gcgctcgcaa
     3121 cgaacccgcc gatcctgatt ttcgatgagg caacctctgc gctcgattac gagagcgaac
     3181 gcatcatcca ggataacatg cggcagatcg taaagaaccg aaccgttttt atcatcgccc
     3241 atcgattggc tgccgttcgc gattgcgact tgatcatcgg catgactgag ggacgaattg
     3301 tcgaggcagg ctctcatgat gatttgatca aaaagccgca cggcctttac gcccggctct
     3361 ggtcgatgca aagcgagagg gctgaggcgt gagcacagtt aaaacaatcg ccaaggctcc
     3421 gccgtccaac gccgtgcaaa agtcgaagct tcatcccgag gatttcgagt ttctgccggc
     3481 ggacctggaa atcttggaga cgccgccatc acctgtacgc atggcgctca ttttgatcat
     3541 ctgcgcattt gtcgcgacag cgttaatttg gagttggatc gggcatatcg acatcgtcgc
     3601 cgtagcgcag ggcaagattc agccaacggg tcgcgtcaag gtcatccaac cgctggaaac
     3661 cggcaaagtc gcggcgatca tggttgagaa cggccggcgc gtaaaggctg gcgacttgat
     3721 gattgaaatg gatatcggcg atgcccgggc ggaggaagac gccacgaaat cagcctatga
     3781 ggctttacgc gccgaggcgc ggcgccggcg cacggcgatc gacgcggcgc agacgcatct
     3841 cctgccacaa gctcgacatc aatcctggaa tgccgaaacg atggaatcgg cgacccagcg
     3901 ccgcttaatg agcattccta agatcgactg ggatgccggg acgccagaac aaatccgaca
     3961 gcgggaaaat caggttctcg ccggcgacct cgagcaactg aaagcgacga tcagcgattt
     4021 cgaggcccag atccgccaga aggagatcga gcacgaccgg cttgatgaag caattgcaat
     4081 gcaagacagg ctgatcgcca ccttgaaaga gcgcgtagat atgcgcgccg tcttagcaaa
     4141 atcgggatca ggctcgaacg cgaacctgat cgatgcaaag gaaacgatgc tgtatcacct
     4201 gtcgcagcag gccaatcaga aagtgcagcg cgacgcgacc attgccaatc tcgacgttct
     4261 ggccggcggg cgcgacaagg ctttctccaa tttcattgca gaaaatgcgc aaaagcttgc
     4321 ggaggcgcaa aagcaggctg acgattggcg ggagaagttg gcgaaggcca aattcaagtc
     4381 ggaacgcatg tccctgaaga gcccgattga tggtgttgtc tatggattat ccgtgacgac
     4441 cataggtcag gtggtgggcg gcggcgagga gttgatgcgc atcgttcccg aagacgcgat
     4501 gctggaaatc gaatgctatc tcgccaataa ggatatcggg tttgtcaaac ccggccagaa
     4561 cgcagtggtg aaaattgaat catttccgtt cacgcgttac ggcacgctgt ccgcgacagt
     4621 aacgcgcgtg gcaagcgacg caataccgga accagacgcg tcccagatgg aaggcaacag
     4681 cgcgcgtcag ccgaaagata agggctttgc tggcgctcag cgcacgcaaa acctcgtatt
     4741 cccggttacg ctgacgcccg cgcgtaaggc catgattgtc gacggtctga cagttccgct
     4801 tactcccggc atggcggtga atgtcgaaat cgcgacgggg aatcgccgaa tacttgagta
     4861 tatcttctca ccactcgtcg aaacggcgtc ccaagcgggg aaggaacggt gagcgttaga
     4921 tcaaccggcc tttaatcgga atcgattaaa ggcggagtgg tggatcgatt tcaaagtcct
     4981 agaagcagcc caagtccgaa tggacgccgg ctggttctaa gctgtaatta caaattgcgg
     5041 tctcaatcat cataattgca acggtatttt ttcgatcgct ccttgagagg caccgtgaga
     5101 cctgccgcgt ttactccctg cgcccaggta tctcgcatgg ctgaataaca gatgcagtta
     5161 tgggccgctc ttctatgctg ttgtcttatt gctgcgagcg gaaaccgcgt ggcatgcgcg
     5221 aacgagaatg cgccaccggt caatctgccg gcccagatcg gagagcgtat tgcgcaatgc
     5281 tggcacgcgc ctcaaacgga cccgccgcaa gtcatcgaag tcacggtgcg gctgagcttt
     5341 tcccgaacgg ggaccctgat cggcgagccg cgtgtcgtct atgttcgcgc ggccaagcag
     5401 gccggattga gagagaaggt cgtaaatagc gtgatcgccg caattaaagc ctgcacgccc
     5461 ttgccattca cgccagcact cggcgctgca atcgccggac gcatctttgc catccgattg
     5521 aattcgctgc cgctgaccgg ccgtcagcgt ttcacctgac tgcggggaaa gttgtgcgct
     5581 ggtaatgccc gaacggtgca gcgcgcggca attgtcgcat cgggtgaaac cagagaggcc
     5641 gccgtctctt ggcgggcatt tttgttggtg cagcgtgagt tcgcattggc ataacggcaa
     5701 tacgattctc ggcttcccag gtttttccga gtgacttgcg atcgccagaa ggcacctttc
     5761 cgtgtgatca ggaggctcca cttttgtcat attccacatt gccgttcgcc catgtgcccc
     5821 aatggcccga tttttgtttg cgcttggcat aattgttgct caaaaagcgt atcatttgct
     5881 ttggcgagag gggtgcttcg caatggcaag caagatacgt cggcggcagc atcggcattc
     5941 cgacttcgac gggaaagggc tgatggctat tcggttggcc tttgcacaca aatttggccg
     6001 ccttccccgc ggggacgatc ctgtcttttt cgatcctgat gcggaccgac cttattcgct
     6061 gccgtcaggc ggaacgcaaa gacttcttct ggaagcaatg ctcgaaaacg gcacggcacc
     6121 tcacatcgtt tacgctttct gccgaacagg ctttgctgtt tccgaccaga acaggaccgc
     6181 tctgccaccg gatcggcttt ccaggtggga cacggcaatc aacgaatatt tggcctttgg
     6241 ggccaaggcg aaacatagtc agcattgaag tgtcgttcac cgtcacattt gcggcgatcc
     6301 gacgcgggta agcagtcgcg ttgataaccg cgcggggcac atcgaggcaa ttgcaacggc
     6361 gatgagctgt tggcctgcga gggaaccggt gttttgccaa gcatccctag attaaacccg
     6421 ccttgaatcg gaatcgatta aaggcggaaa agttggtcga ttccaaagtg ctagagcagc
     6481 ttatccgcgt ccaaatagac gcgggcggct ccattggagt gaatttgaca ttgggtgcca
     6541 cctgccgcga gttcgcgctc aagtgtcaaa ttcaaaagtt ctaatagaat caaaaagata
     6601 ccagtggtcc ttttgattcc acaaatgtcg ggttcgcact gggacgctga cctcaaacag
     6661 aacccgtttt ccgaacattg cccctgcgga accggataac catgctaaga taggcttcac
     6721 cgtcagccgt acagggcctg acggtgggca aaatttgggt ggttggaatg acaagctctg
     6781 cgtcggttac ctatctaacg cggcagttcg acgacttttt atttgcgcga atcgacgagg
     6841 gtagcaatcc aacgccgctc agtgtgcttt caatgctggc gcgccttgat atcgatccat
     6901 gggaggaggc ggctaaactt gctcgattgc cgcgcgcagc ggcggccagg aggcttgtcg
     6961 atttcattgc agcgacaccc ggtgccccat cggcatatct aaacgccaag acggtttccg
     7021 atcggctgct caatcttttg ccatcgcctg ctcttcctag gcaggaacaa gcgacgcgcg
     7081 cgttgaagaa ggtcccgttc gtctggccgg ccgttattgt cgttgctatt ctggcgatct
     7141 cgctgatcgt catgcgcctt taaag
//
LOCUS       DATMGX010000301         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_172000, whole genome shotgun sequence.
ACCESSION   DATMGX010000301 DATMGX010000000
VERSION     DATMGX010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172000"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VNR65_04885"
     CDS             complement(<1..439)
                     /locus_tag="VNR65_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HWJ18048.1"
                     /translation="MKKNEMPDAPSSPPPISGLSDAEVKRRQASEGFNELPRPDQRTT
                     LRIILEVLREPMLALLLGGGVVYLLLGDLKEAVILLVFAIMSVAITVVQESRTERVLE
                     ALRDLTSPRALVIRDGERKRVAGREVVRGDFVVLAEGDRVPADA"
     gene            926..1375
                     /locus_tag="VNR65_04890"
     CDS             926..1375
                     /locus_tag="VNR65_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18049.1"
                     /translation="MNKLNAAIVLSLFLGGAFASNAQTVAPTLDRVPPDADPLRAALP
                     EVKKVILAATGYESGSVEVSATSRDIVVTLANSSLAAGSNIGRESEARSIALAIASAI
                     ASRQEFQGIVALHIDYTTREPDGSHSRTIDKIDFRKDPGGSFQHHIS"
     gene            1438..1572
                     /locus_tag="VNR65_04895"
     CDS             1438..1572
                     /locus_tag="VNR65_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18050.1"
                     /translation="MTSTLGAERAKVWDPLVRLGHWALVIGFAIEWSLLQVIPIARTS
                     "
BASE COUNT          468 a          599 c          582 g          438 t
ORIGIN      
        1 ctgcatcggc cggaacccgg tcgccttcgg ccagcacgac aaaatcgcca cgcaccacct
       61 cccggccggc aacgcgtttg cgctcgccgt ctcgaatgac cagggcacga gggcttgtga
      121 ggtcgcgaag ggcttcgagg acgcgctcgg tacgactctc ctggacgacc gtaatcgcaa
      181 ccgacatgat cgcgaacacg agcaggatga cggcttcctt caggtcgcct agcaacaggt
      241 agacaacgcc gccgccgagc agcagagcca acattggctc gcgcaacact tcgaggatga
      301 tgcgcaatgt cgttcgttga tcgggccggg gtagctcgtt gaacccctcg gaggcctgcc
      361 tgcgtttcac ctcggcgtcg ctgagacctg agatgggtgg aggggatgat ggagcgtcgg
      421 gcatttcatt cttcttcatt gttcttgatc tcacaacact ttccaatact ccggcccagc
      481 gaatgtgaat aggcgctgcc aacgggaccc ccgcaatcaa cgctagatca ggcgcctatt
      541 cagccggtag gcgtgcaatc tgtgctccaa ttcacaacca tcggccaaga gtgtgcatgt
      601 gggcgcaatg aattttccga agccgcagcc agccaacgca tctattgttt ttggaggtac
      661 tcatagaaca taccaaaaca gcgagtatta ttctccttaa attctctttc ctaccgagac
      721 atctgctctg agtattttcc gagtctatga aaatcaattg gcaaatgtct aagctgcggc
      781 catctgaaca gcgccctcgt caaatcaagt ggcatcggca agcttgaatg atgaaacggg
      841 catccctcgc atcgagacag cgagaagcgg cagtcagtct ccgatgagaa tcggcgcgtc
      901 tgaaaatttt cacaaaagga tttcgatgaa caagctcaac gccgcaatcg tgctgtcact
      961 attcttaggc ggcgcctttg cctcgaacgc gcaaaccgtg gcgccaactc tcgaccgggt
     1021 tcctccagat gccgacccgc tccgcgcggc gctccctgag gtcaagaaag tgatcctcgc
     1081 cgcaaccgga tacgagagcg gaagcgtcga agtgtcggcc acgagccgcg atatcgtcgt
     1141 gaccctggcg aacagtagtc tggctgctgg atcgaatatt ggtcgagaaa gtgaagcccg
     1201 gtcaattgcc ttggcgatcg ccagtgcaat agcaagcagg caggagttcc agggcattgt
     1261 ggcgcttcac attgactata cgacacgcga gccggacggc agccactcgc ggacgataga
     1321 caagattgat ttccgtaaag accccggggg cagtttccaa caccacatta gctagcgcgc
     1381 ttgggccgca gtcgtgcgcc aacccagaag gcccggaaac aattcggaga acgaccgatg
     1441 acatccactc taggcgcgga gcgagctaaa gtctgggatc cgcttgtgcg gctcgggcat
     1501 tgggcgctcg ttatcggttt cgcgatcgaa tggtcgcttc ttcaagttat tcccatcgcg
     1561 agaacctcgt aagcgcaatg ttcaccggag aaaaacgcgc gaacgggtca tcggagtgag
     1621 acgatccggc ttctttagaa attgggttcg ccctgggcag ttcagtcaaa ttacgaggct
     1681 tccactcaag gaaaggcgcc cgttctctcg cgcgaatgcg ctgctcgcat agcgtaaaga
     1741 ttgatccttc gcgccgcgac acaacggttg atgcccgcgt cattcgtatg cccgctggtg
     1801 ggacatttat cccactccac gttttcacgc agccagggtc agggtagcat caacaagacg
     1861 gcttaccctg cagtagccga agggtttttt gcaagtgcga gaaatggcgg cggggtgatt
     1921 tgtggttctt gggttcggtc tcgatgcaca ttatcgcctg gcggcttatt tcacgctggc
     1981 gctgcggctc tcacgtggca taacggcggc aaatctaccc agcatcctcg gtggtcatgg
     2041 ttcgtctcga aaacatcagc aagcagaatg gcaatcacct cctgttc
//
LOCUS       DATMGX010000302         2159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_73526, whole genome shotgun sequence.
ACCESSION   DATMGX010000302 DATMGX010000000
VERSION     DATMGX010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73526"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VNR65_04900"
     CDS             <1..868
                     /locus_tag="VNR65_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ18051.1"
                     /translation="FLTLPKGSDTEHPGRPDQPLPMVLFVHGGPWSRDHLGYNAMHQW
                     LANRGYAVLSVNFRSSTGFGKAFVNAGDKEWGRKMDDDLLDGVAWAIREKIADPARIG
                     IMGGSYGGYATLAALTRNADTYACGVDLVGPSELDRLIKTIPPYWASGRARIIKSIGD
                     PDTEEGRALLKERSPLYQADRIKKPLLIAQGENDPRVKKEQSDQIVETMQSKNIPVTY
                     LLYRREGHGFAEPENNISFTAIAESFLSKCLGGRVAPITPADLKGTSVEIPVGAELIE
                     GYSTAKKSASAQ"
     gene            1362..>2159
                     /locus_tag="VNR65_04905"
     CDS             1362..>2159
                     /locus_tag="VNR65_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWJ18052.1"
                     /translation="MTKIDVPLDSRLRLLSYADAHGHPRLGLVRADGHVVDVANAAQQ
                     TKTALGFDGKSMLALIEAGPQGLAEVRELAAADVHTDLKVEDVRLLPPIPKLTRNIVC
                     VGWNYLEHFAEGAKMRDASQKLPEHAVFFTKATGSLNGPFDPIPYDPSVSREIDWEVE
                     LAVVIGKRGRNISEADAADHVFGYTVLNDVSARDLQKTAHGGQWFKGKSLDGHAPMGP
                     WLIPGADIEPDNLRLSLRVNGAVKQDGNTKDMYFKIPRIIAELSRGLT"
BASE COUNT          437 a          615 c          686 g          421 t
ORIGIN      
        1 gtttctcacg ctgcccaagg ggtccgatac ggagcacccc ggcagaccgg accagcccct
       61 gcccatggtg ctgttcgtgc atggcggccc gtggagccgc gatcatctcg gctataacgc
      121 gatgcatcag tggcttgcca atcgcggtta cgcggtcttg agcgtgaatt tcagaagttc
      181 gaccggcttc ggaaaggcgt tcgtgaatgc gggcgacaag gaatggggcc gcaagatgga
      241 tgatgatctt ctggacggcg tggcctgggc catccgcgaa aagatcgccg atccggcgcg
      301 gatcggcatc atgggcggca gctatggcgg ctacgctacc ctcgctgccc tcacccgaaa
      361 cgcggacacc tacgcttgcg gcgtcgatct cgtcggccct tccgaactcg accgcttgat
      421 caagacgatt ccgccttatt gggcttccgg gcgcgcaagg atcatcaagt ccatcggaga
      481 tcccgatacc gaagaaggtc gtgcgctgct gaaggagcgc tcgccgcttt atcaggcgga
      541 caggatcaag aagccgctgc tgatcgcgca aggcgagaat gatccgcgcg tcaagaaaga
      601 gcaatcagat cagatcgttg aaaccatgca gtctaaaaat atcccggtga cctatttgct
      661 gtaccggcga gagggacatg gctttgccga gcctgagaac aacatctcgt ttaccgctat
      721 cgccgagtct ttcctcagca aatgcctggg cggcagggtc gcaccaatca ctcctgcgga
      781 tctgaaagga acgtcggtgg aaattcccgt cggtgccgag ctgatcgagg gctattctac
      841 agcaaagaag agcgcatctg ctcaatagct ccacgagagg aaaacttcat gatcgaccgg
      901 ctggaatttc ctatagacgg caagtgcgcc tcaccgctcg gacaaagacc atgccggtcg
      961 tgaagcctgc gacggtgata tcgctcttgg ctcggcggca gacgtagaca aggcgccgct
     1021 tgcgggctcg gactgtgaat gtgaacggcg catcgcttga gatggccgcg ccgttcggtg
     1081 gccgaggagt ccggcatggg ccgcgagtgg agccggtttg gcctggagga gtttctcgaa
     1141 gtgaaagccg ttgccggctg gggggagtga agcgcggccg catttgttcg gaagagatag
     1201 acccctcatc ctgtccttct cccggcaagc tcgccttggc gagcttgcaa cttaaagtga
     1261 ccgcatccgc tcgcggatgc ggttggggag aaggaacgct gtttctcctc tcccccggcg
     1321 ggagaggggc gggggtgagg gggcaaaccc aaggataaaa gatgacaaaa attgatgtgc
     1381 cgctcgattc aaggttgcgt cttctctctt atgccgatgc acacggccat ccgcgtcttg
     1441 gcttggtccg cgctgacggc catgtggtcg acgtcgccaa tgctgctcaa cagacgaaaa
     1501 ccgcgctcgg tttcgatggc aagtccatgc tcgcgctgat cgaggccggt ccgcagggct
     1561 tggcggaggt ccgtgagctt gctgccgcgg atgtccatac ggacttgaag gtggaggacg
     1621 tccggctctt gcccccgatc ccgaaattga cgcgcaacat tgtctgcgtc ggctggaatt
     1681 atctggagca tttcgcggag ggcgcgaaga tgcgcgacgc atcgcagaag ctgccggaac
     1741 acgctgtgtt cttcaccaaa gcgacgggat cgctcaacgg gccgttcgat ccgatccctt
     1801 atgatccatc ggtttcgcga gagatcgact gggaggtcga gcttgcggtc gtcatcggca
     1861 agcgcgggcg gaacatctcc gaggccgacg cggcggatca tgtcttcggc tatacggtgc
     1921 tgaacgacgt ttcggcgcgc gatctgcaaa agactgcgca tggcggccag tggttcaagg
     1981 gcaaaagcct tgatggccat gcgccgatgg ggccgtggct cattccaggg gccgacattg
     2041 aaccggacaa tctccgcctg agcttgcgcg tgaacggcgc cgtcaagcag gacggcaata
     2101 cgaaggatat gtatttcaaa atcccccgca ttatcgccga gctctcgcgc gggctgacc
//
LOCUS       DATMGX010000303         2737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_106721, whole genome shotgun sequence.
ACCESSION   DATMGX010000303 DATMGX010000000
VERSION     DATMGX010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106721"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VNR65_04910"
     CDS             <1..482
                     /locus_tag="VNR65_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWJ18053.1"
                     /translation="KSKLIRQTEMEDFMPMHKFLEWHASQFMTKNVKTVPSTMTLREL
                     GELFDKNDFNSFPVIDNGELIGLVTKLDFLKAFLFDTGHLVPHYNEVMTLTVAKIMTK
                     DVVHAEPETPLTRVLELMVKLRTRGVPVLDKGKLVGVVSRTDLMHALSTATKDDERS"
     gene            complement(483..1466)
                     /locus_tag="VNR65_04915"
     CDS             complement(483..1466)
                     /locus_tag="VNR65_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HWJ18054.1"
                     /translation="MRAMVLKAPRQPLELEIREIPRPKPGELLLKVRACAVCRTDLHV
                     VDGDLPPCRATIVPGHEIIGEVAGLGDGVVGFKAGQRVGVGWLGHTCGHCPFCTHDEE
                     NLCDYAEFTGYQRDGGFADYVVADAAFSFPIPDSYGDSEAAPLLCAGLIGYRSLKMAG
                     PGKRLGIYGFGAAAHIVAQVALWEGRSVYAFTRQGDAAAQHFARSLGCCWAGDSSERP
                     HADLDAAIIFAPVGGLVPTALKAVRKGGIIVCGGIHMSDIPSFPYSILWGERVVRSVA
                     NLTRLDATDFLALAPQVPVKSTVQIFPLTEANEALSQLRDGKLTGAGVLIP"
     gene            1601..>2737
                     /locus_tag="VNR65_04920"
     CDS             1601..>2737
                     /locus_tag="VNR65_04920"
                     /inference="COORDINATES: protein motif:HMM:NF017873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HWJ18055.1"
                     /translation="MTIEQQTGYSDYPVTQEARFNLIGGGIASLAAAVFLIRDGKIAG
                     RNITIFGDQPKPGGSLEGAGSPETGYIARGGRVLEAHYVCTYDLFSSIPALDGSRSVT
                     QEIFACNEALKTYSHARLVREGRKLASPEFGLTEGHRLRLIGVALSPESVLGSSAIED
                     HFDKAFFKTDFWAMWATTFAFQPWHSAVEMKRYLMRFVHMMQGLNRLQGIMRTTYNQY
                     DSMVRPLAKWLDDKGVRFVTGTVVSDIQFERVKEAQRPSALILQREGRTEEIKLSVDD
                     YVIVTLGSMADSATLGDMNKPAPYQPEKKGAGWHLWERIAEGRPEFGKPSAFDSRPDQ
                     SKWVSFTGTLRDPAFFRIVKEFTGNVPGEGGLITFADSGWLLSIT"
BASE COUNT          595 a          834 c          791 g          517 t
ORIGIN      
        1 tcaaatctaa acttattcgt cagaccgaaa tggaggactt catgccgatg cacaaattcc
       61 tggagtggca tgccagtcag ttcatgacaa agaatgtcaa gaccgtcccc tccaccatga
      121 ccttgcgcga gcttggcgaa ctgttcgaca aaaacgactt taattccttt cccgtcatcg
      181 ataatggcga actcatcggt ctggtgacga agctcgactt ccttaaggcg tttcttttcg
      241 ataccggtca tctggtcccg cactacaacg aagtcatgac attgacggtg gcgaagatca
      301 tgacaaagga cgtggtccac gctgaaccgg aaacgccgtt gacccgggtg ctggaactca
      361 tggtcaagct gagaacgagg ggcgtccccg tcctcgacaa agggaagctt gtcggcgtcg
      421 tatcgcggac agacctgatg catgcgctct cgaccgccac aaaagacgac gagcgctcgt
      481 gattaaggga tgagcacacc cgcgccggtc aatttgccgt cccgcaattg ggagagggct
      541 tcgttggctt cggtcagggg gaaaatctgg accgtcgatt tcacaggcac ctgcggtgcg
      601 agcgcaagga agtccgtcgc atccagccga gtaaggtttg ccaccgagcg caccacgcgc
      661 tccccccata agatgctata gggaaaagac gggatatccg acatatggat cccgccgcaa
      721 acaatgatgc cgcccttcct gactgccttc aatgcggtag gtaccagccc tcccacaggt
      781 gcgaagatga tcgctgcgtc gagatcggca tgcggccttt cgctcgagtc gccagcccag
      841 caacatccga gcgaccgcgc gaagtgctgc gccgccgcat ccccctgccg cgtgaaggcg
      901 taaacgctgc ggccttccca gagcgcgacc tgtgcgacga tatgcgccgc tgctccgaag
      961 ccatagatgc cgagcctctt tccggggccg gccattttga gagagcggta tccaatgaga
     1021 cccgcacaaa ggagcggcgc cgcctccgag tcgccataag agtcgggaat tgggaaagaa
     1081 aaggccgcgt cggcaaccac gtaatcggcg aagccgccat ctctctggta gccagtgaat
     1141 tcagcgtaat cgcagaggtt ttcctcgtca tgcgtgcaaa aggggcagtg gccgcaggtg
     1201 tgtccaagcc agcccacgcc gacgcgctgc cctgccttga agcctacgac accgtcgcca
     1261 aggcctgcta cttctccgat aatctcatgg ccgggaacga tggttgcgcg gcagggagga
     1321 agatcgccgt cgacgacatg caggtctgtg cggcaaaccg cgcaggcccg cactttcaac
     1381 aaaagctccc ccggcttggg ccgcggaatc tcgcgaatct ccaactccaa aggctggcgc
     1441 ggcgctttca aaaccatcgc tctcatggga tgtcctccgc aagaaaatgc aggccaatca
     1501 taccattgaa aactggcatg taagggcttg acatcaatca aggtattctt tgcggcaata
     1561 gacaatttag gcatgggcct cagaccgaag gagccgagcc gtgaccatag aacagcaaac
     1621 cggatattca gattatccag ttacccagga ggcccgtttc aatctcatcg gcggagggat
     1681 cgcctcgctc gcggcagcgg tttttctgat ccgcgacggc aagatcgccg gccggaacat
     1741 caccatattt ggcgatcagc cgaagccagg cggcagcctt gagggcgccg gctcgcccga
     1801 aacaggttat atcgcccgcg gcggccgggt tctcgaggcg cattatgtct gcacctacga
     1861 cctgttctcc tcgattcccg ctctcgacgg ctcccggtcc gtcacgcagg aaattttcgc
     1921 gtgcaacgag gcgctgaaaa cctattcgca tgcgcgcctc gtgcgcgagg gccgcaagct
     1981 cgcctcgcca gagttcgggc tgaccgaagg gcaccgcctc cgcctcatcg gtgtcgcgct
     2041 cagtcccgaa agcgtgctcg gatcgagcgc catcgaggac cactttgaca aggctttctt
     2101 caagacggac ttctgggcga tgtgggcgac gaccttcgcc ttccagccct ggcacagcgc
     2161 cgtcgagatg aagcgctacc tcatgcgctt cgtgcacatg atgcaggggc tcaatcgcct
     2221 ccagggcatc atgcggacca cctacaacca atatgattcg atggtcaggc cgcttgcgaa
     2281 atggcttgat gataaaggag tccgcttcgt cacgggcacc gttgtgtccg acatccagtt
     2341 cgagcgcgtt aaggaagctc agcgcccgtc cgcactcatc ctgcaacgcg aaggacgcac
     2401 cgaagagata aaactctccg tcgacgatta tgtgatcgtg accctcgggt cgatggcgga
     2461 ttccgcaacg ctgggcgata tgaataagcc cgcgccttac cagccggaga aaaagggcgc
     2521 ggggtggcat ctttgggagc ggattgcgga aggcaggccg gagttcggaa agccctcggc
     2581 cttcgacagc aggcccgatc agtcgaagtg ggtgtcgttc acgggcacgc tgcgcgatcc
     2641 ggccttcttc cgcatcgtca aggaattcac gggcaatgtc cccggtgaag gcgggttgat
     2701 caccttcgcg gactcgggct ggctcctctc gatcacg
//
LOCUS       DATMGX010000304         2055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_261731, whole genome shotgun sequence.
ACCESSION   DATMGX010000304 DATMGX010000000
VERSION     DATMGX010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261731"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VNR65_04925"
     CDS             complement(<1..843)
                     /locus_tag="VNR65_04925"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HWJ18056.1"
                     /translation="MSEGPRLRESFRREPHPLPDGWNWTWQNEVFTFKGGAQPPASQF
                     MDHSAAGNIRFVQIRDYYSSDHLTYVPDLPNLRKCGASDVMIARYGASLGRICRGIEG
                     AYNVALVKVSPIPPTENDFLFYLLQSEYFQAPMQGQGARSAQAGFNREGLKNILLPLP
                     RPGEQRAIAEVLSALDERIAANAKVAQTSLALASSIFLDSIARVPFSDHTFNELADIG
                     GGGTPRTDTPEFWDGEVPWATPTDITALTGPYLESTPRKISDLGLASCSSPLYPAGSI
                     LMTSR"
     gene            complement(836..>2055)
                     /locus_tag="VNR65_04930"
     CDS             complement(836..>2055)
                     /locus_tag="VNR65_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWJ18057.1"
                     /translation="AATLPRIYNRDNVDQRRLGELLDLFNSARFTGQGVSKARDLLGE
                     VYEYFLEKFAKAEGKRGGEFYTPASVVRVLVEVLEPTHGRVYDPCCGSGGMFVQTEKF
                     LEAHNREGSNISVYGQELNERTWRMARMNLAIHGINGKLASRWGDTFARDQHPDIQAD
                     YVLANPPFNIKDWARSDKDPRWKFGVPPINNANYAWIQHIVSKLAPGGSAGVVMANGS
                     MTSNSGGEGEIRAEIVEADLVSCMVALPTQLFRSTGIPVCLWFFAKDKSKGKGKGKGG
                     AIDRTGQVLFIDARNLGFMVDRAERSLADADIAQIASTFHAWRGTASARDAGLTYEDQ
                     AAFCYSASLAEIKAADYALTPGRYVGAADVEDDGEPITDKIERLTKQLFKEFDESARL
                     AIIVREQLGRVGV"
BASE COUNT          400 a          648 c          585 g          422 t
ORIGIN      
        1 tcgcgacgtc atcagaatcg agccggcggg atacagtggc gaggagcagc tcgccagacc
       61 cagatcactg atttttcttg gtgttgactc gaggtatggc cctgttaagg ccgtgatatc
      121 cgtgggagtt gcccacggca cttcaccgtc ccagaactca ggcgtgtcag ttcgaggcgt
      181 acccccgcct ccgatgtcgg ccagctcatt gaaagtgtga tctgagaacg gcaccctggc
      241 tattgagtcc agaaaaatcg acgaagcaag agccagggac gtctgggcga ctttggcgtt
      301 ggcggcgatc ctttcgtcga gggcacttaa cacctccgca atcgctcgct gttcacccgg
      361 ccgaggcagc ggaagtagaa tattctttag gccctctcta ttgaacccag cttgggcgct
      421 acgcgcgcct tggccctgca tcggagcttg gaagtactct gactgcagca gatagaacag
      481 gaagtcattc tcggttggag ggatcggtga gaccttgaca agtgctacgt tgtaggcacc
      541 ttcgattcca cgacaaatcc tgcctaacga agccccgtat cttgcgatca tcacgtcgct
      601 cgccccgcac tttcgcaagt ttggaaggtc aggcacatag gtcagatgat cacttgaata
      661 gtagtcgcga atttggacga agcggatgtt tccggcagcg gaatgatcca tgaattgaga
      721 cgcgggcggc tgtgctccgc ctttaaaggt aaagacctcg ttctgccatg tccaattcca
      781 gccatccggc aacggatgtg gttctcgccg aaacgattca cgcaagcgcg ggccttcaga
      841 caccaaccct cccgagctgc tcgcgcacaa ttatggcgag ccgcgccgac tcatcaaact
      901 ccttgaacag ctgcttcgtt agccgctcga tcttgtcggt gattggctcc ccgtcgtcct
      961 caacgtccgc tgcacccacg tatcgcccag gtgtcagcgc gtagtccgcc gccttgatct
     1021 cggccagcga ggccgaatag cagaacgcag cctgatcttc gtaggtcaac cccgcatcgc
     1081 gagccgaggc ggtaccccgc caggcatgga aagtggacgc gatctgagcg atatccgcat
     1141 ctgccaacga ccgctccgcg cggtccacca tgaacccgag gttccgagcg tcgatgaaca
     1201 gcacctgccc ggtccggtcg atagcgccac ccttgccctt gcccttgccc ttggacttgt
     1261 ccttggcgaa gaaccacagg cacaccggga tcccggtgga ccggaacagc tgggtcggta
     1321 gtgcgaccat gcacgacacc agatcagctt ccacgatttc cgcacggatc tcaccctccc
     1381 caccggaatt cgaggtcatg gagccattcg ccatcaccac cccggcgctc ccgccgggtg
     1441 ccaacttcga gacgatgtgc tggatccagg cgtagttggc gttattgatc ggcggcacgc
     1501 cgaacttcca gcgcgggtcc ttatcgctgc gtgcccagtc tttgatgttg aacggcgggt
     1561 tggccagcac ataatcggcc tggatatccg ggtgctggtc ccgggcgaat gtgtcacccc
     1621 agcggctggc gagtttgccg ttgatgccat ggattgcgag gttcatcctt gccatccgcc
     1681 aggtgcgctc gttgagttcc tggccgtaga cagagatgtt cgaaccctcg cggttgtgcg
     1741 cctccaggaa cttctccgtc tgcacgaaca tgccaccgga cccgcagcat gggtcgtata
     1801 cccggccgtg ggtcggctca agaacttcca ccagcacccg aaccacactg gccggcgtgt
     1861 agaactcgcc gccacgtttg ccttctgcct tggcaaactt ctccaggaag tactcgtaca
     1921 cctcgccgag gaggtcgcgg gccttcgaaa cgccttgccc ggtaaagcgt gcgctgttga
     1981 acaggtcgag cagctcaccg agccggcgtt gatcgacatt gtcccggttg tagatccgcg
     2041 gcagcgtcgc cgcca
//
LOCUS       DATMGX010000305         2875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_424945, whole genome shotgun sequence.
ACCESSION   DATMGX010000305 DATMGX010000000
VERSION     DATMGX010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424945"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /gene="ispH"
                     /locus_tag="VNR65_04935"
     CDS             <1..635
                     /gene="ispH"
                     /locus_tag="VNR65_04935"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWJ18058.1"
                     /translation="VTKVHLQGKRYLSLGRTVILVGHAGHPEVEGTMGQIPGPVHLVQ
                     TEDDVSALPIPVHTPVAYVTQTTLSIDDTRGVIDAIKRRFSNVIGPETNDICYATQNR
                     QMAVRELTKIADVILVVGAKNSSNSNRLREIGEEAGIPSYLLAEGSELKPEWVRGAQV
                     IGLTAGASAPEDMVQDVIASLRLLGPVDVTILPGVEENVEFRLPAELHAF"
     gene            823..1965
                     /gene="hpnH"
                     /locus_tag="VNR65_04940"
     CDS             823..1965
                     /gene="hpnH"
                     /locus_tag="VNR65_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HWJ18059.1"
                     /translation="MVLPFFQERRIGAYLLKQKLKGRKRYPLVLMLEPLFRCNLACAG
                     CGKIDYPDSILNRRLTVQECLDAAEECGAPMVAIPGGEPLIHKEIGEIVAGLVAQKRF
                     VSLCTNALLLEKKLHLFEPSPYLFFSVHLDGLRDHHDKSVCQPGTFDRAFAAIRAAKA
                     KGFKVNVNTTVFDDYKPEELAFFLDFCKDLDVGVSISPGYAYERAPDQEHFLNRRKTK
                     ELFRRVFALGKGKRWTFSHSPLFLDFLAGNQQFSCTPWGMPTRNIFGWQKPCYLIGEG
                     FVKSFTELMETTDWDSYGTGRYEKCANCMAHCGYEPTAAHAAISNPLKMLAVALRGIR
                     TEGPMAPEIPLENQRPAAYVHSLHVEREVQRIQKTEERPERLTAAE"
     gene            complement(1888..2595)
                     /locus_tag="VNR65_04945"
     CDS             complement(1888..2595)
                     /locus_tag="VNR65_04945"
                     /inference="COORDINATES: protein motif:HMM:NF005476.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HWJ18060.1"
                     /translation="MKMLFDDAPVIVAAGMAFEARIAKGSGIRAVYGQKREVYLRKLH
                     EFARTGARGIISFGVAGGLSPSLKPGDVVVASSVVTAKGSFPADPRWSRSLHNALEHA
                     VSRPIFAADATVMTVLEKEALWKGTGAAAVDMESGAAAEVAELYGLPFAALRVIIDPA
                     HRTIPVSAAVGARENGTTDAFAVIRSLLRRPHDLGEIIRLADDARRANRSLFRCRQAL
                     GPFFSLLDPLDLALNVQ"
BASE COUNT          635 a          824 c          824 g          592 t
ORIGIN      
        1 ttgtgacgaa ggtgcatctg cagggcaagc gttacctgag cctgggacgt accgtaattc
       61 tcgtcggcca tgcgggacat cccgaggtgg aaggaacgat gggtcaaatt ccgggtccgg
      121 tccatcttgt gcaaaccgaa gacgacgttt ccgctttgcc tatcccggtg cataccccgg
      181 tcgcttacgt aacccagacg acgctcagca ttgatgacac ccgcggtgtc atcgacgcga
      241 tcaagcgccg cttttcgaat gtgatcgggc cagagacaaa cgatatctgc tacgcgacgc
      301 aaaatcggca aatggcagtc cgagaactga cgaaaatcgc ggacgttatc ctcgtcgtcg
      361 gcgccaagaa cagctcaaac tcgaacaggc tgcgggaaat cggcgaggag gcgggcattc
      421 caagctatct cctggccgaa ggtagcgagc tcaaaccgga gtgggtgcga ggcgctcaag
      481 tcatcggctt gaccgcagga gcttctgctc ctgaagacat ggttcaggat gtcatcgcaa
      541 gtctgcggct tctcggaccg gtcgacgtta caatcctgcc aggggtcgaa gagaatgtgg
      601 aattccgcct gcccgcagaa cttcacgcct tttagatcaa cccgccttta atcgaaatcg
      661 gttaaaggcg ggttgattga ttctaagtgc tagagcagcc agcgtccaaa tggacgcgga
      721 ctggcctagc gtccagggga acccatcccg tttcactgga ttcacctatt gaaatcgcct
      781 aaacgcgtgt ccacgcggca ttttttgaat tggagcagca ctatggtctt accgtttttc
      841 caggaaagac ggattggcgc ctatcttctc aagcagaagc tgaagggccg gaaacgttat
      901 cccttggtgc tgatgctcga gccgctgttc cggtgcaatc tggcttgcgc gggatgcggc
      961 aagatcgact atcccgactc catccttaac cgtcgcctga ccgtgcagga atgccttgat
     1021 gccgcggagg aatgcggcgc tccgatggtg gcgatcccgg gcggggagcc cttaatccat
     1081 aaggagatcg gtgagatcgt cgcggggctc gttgcccaga aacgcttcgt gtcgctctgc
     1141 acgaacgcgc ttctgcttga gaagaagctt catctgtttg agccgtcacc ctacctcttc
     1201 ttctcggttc acctcgacgg cctgagggat catcacgaca agtcggtgtg ccagcccggc
     1261 actttcgaca gggcgtttgc ggcaataagg gcagcgaagg cgaaaggctt caaggtcaac
     1321 gtcaacacga cggtcttcga tgactataag ccggaggaac tcgccttttt cctcgatttc
     1381 tgtaaagacc tcgacgtcgg cgtctcgatc tcgcccggct atgcctatga gcgtgcgccc
     1441 gatcaggagc acttcctcaa tcgccgcaaa accaaggagc tgttccgacg ggttttcgca
     1501 ctcggaaagg gaaagcgctg gacgttctcc cactcgcctc tctttctgga ctttcttgcg
     1561 ggcaaccagc agttctcatg cacgccttgg gggatgccga cccgtaatat ctttggctgg
     1621 cagaagccct gctatttgat cggcgaaggc ttcgtcaaat cgtttacgga actgatggaa
     1681 acgacggact gggacagcta cggcaccggc cgctatgaga agtgcgcgaa ctgtatggct
     1741 cattgcggct acgagccgac tgctgctcac gcagctattt ccaatccgct caagatgctg
     1801 gcagttgctc tacgcggcat ccgcaccgag ggcccgatgg ctcccgagat cccgctcgag
     1861 aatcagcgcc cggcggctta cgtgcattca ctgcacgttg agcgcgaggt ccagcggatc
     1921 cagaagactg aagaacggcc cgagcgcttg acggcagcgg aatagagatc tgttcgccct
     1981 gcgggcatcg tccgcaaggc gaatgatttc accaaggtcg tggggccttc gcagaagcga
     2041 ccggatcacg gcgaacgcgt cggtcgtgcc gttttcgcgt gctccgactg ctgcgctgac
     2101 cggtatcgtc cgatgagctg gatctatgat gactctcagg gctgcgaaag gcagtccata
     2161 gagctctgcg acttccgcag cggcgcctga ttccatgtca acagcggcgg cgcctgtccc
     2221 cttccacagc gcttccttct caagcacggt catgaccgtt gcgtcggcag cgaagattgg
     2281 gcgtgaaacg gcatgttcga gggcattgtg gagcgaccgc gaccagcgtg ggtctgcagg
     2341 aaagcttccc ttcgcagtga caacggaact ggcgacgacg acgtcccccg gcttgagcga
     2401 cggtgacaga ccgccagcca cgccgaaact aatgattccc ctggcgcctg tccgggcaaa
     2461 ttcgtgaagc tttcggagat agacttcgcg tttctgccca tataccgcgc gaatccctga
     2521 acctttcgcg atacgcgctt cgaaggccat gccagcggcc acgatgaccg gagcgtcgtc
     2581 gaaaagcatc ttcattgaag tagcccgccc gtcttagaaa tgcccgcagc aaagaagcgc
     2641 tgcgcaacaa aggcgtatac aaaacctcgc ctttgaggac aagcaagctt aacgaaaggt
     2701 taaatgttgt cgccgaagcg tgacatgcaa agcacagaag ctacatgccg aacattacag
     2761 gcgatcgatt tccagccttg agatttcgat agcgtgcgag cgcccagagc ggaaaatatt
     2821 tcccataacc gtgataaata agataaaaaa cccgtgggaa gcctggagca ttatg
//
LOCUS       DATMGX010000306         6186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_222924, whole genome shotgun sequence.
ACCESSION   DATMGX010000306 DATMGX010000000
VERSION     DATMGX010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222924"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(702..1682)
                     /locus_tag="VNR65_04950"
     CDS             complement(702..1682)
                     /locus_tag="VNR65_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HWJ18061.1"
                     /translation="MKTIVIREPGGPEVLTCQDLATPEPLAGEVLIKVAAAGVNRPDV
                     LQRMGLYPPPPGVSNIPGLEVAGEIATLGEGVSQWRVGEKVTALTAGGGYAEYALAHE
                     GSCMRVPKGLSIADAACIPETFMTVWHNVFERGALKAGESFLVHGGSSGIGSSAIQLA
                     AHFGARVFATAGSDEKCAYCEKLGAERAINYKTQSFAKEIKDLTCKRGVDVILDMVGG
                     AYVQDDIACAAEDGRIVQIAFLKGARVQLDLMPLMLKRLALTGSTLRPRKLEFKARIA
                     KELQKKVWPLLESGAVKIVIDKTFPLDQAADAHRRMESNAHMGKIVLEVG"
     gene            1987..2514
                     /locus_tag="VNR65_04955"
     CDS             1987..2514
                     /locus_tag="VNR65_04955"
                     /inference="COORDINATES: protein motif:HMM:NF018972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HWJ18062.1"
                     /translation="MKSSCVSQKFQNTALLSFGDRFTTSPQFMKLFREGMDLVEETAE
                     YLDGAGRAESKQLAPPASLAYTSESIRLTTRLTQLASWLLVRRAIASGEITAAQAHTH
                     RHRVTLSPQSAVHPEGFDALPESFKRLIAESFRLHDRITRLERILGDSPSRADKSPSP
                     IDGHIERLRMEFPAA"
     gene            complement(2524..2706)
                     /locus_tag="VNR65_04960"
     CDS             complement(2524..2706)
                     /locus_tag="VNR65_04960"
                     /inference="COORDINATES: protein motif:HMM:NF018413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HWJ18063.1"
                     /translation="MDIHDLEPRKQPSKPKDLTVYSVVDLQAYVELLKAEILRAETVI
                     KQKDAHKDAASAFFKK"
     gene            complement(2926..3774)
                     /gene="puhE"
                     /locus_tag="VNR65_04965"
     CDS             complement(2926..3774)
                     /gene="puhE"
                     /locus_tag="VNR65_04965"
                     /inference="COORDINATES: protein motif:HMM:NF023711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative photosynthetic complex assembly protein
                     PuhE"
                     /protein_id="HWJ18064.1"
                     /translation="MLPVLFVLFVWWFSTGAVIYLNNLPKYTYKWSFLGATFVLVASI
                     YGLTLTSQNDSIVSAYLSFTFGLLAWAWTQFTFYTGMITGPRTTPCPSSCKGSRHFWH
                     AVETSMYHEITAALLGVVICFSTMTGKNHVGSWTYTIIWLMHSSAKINAVLGVRNLND
                     HFFPEQLRYLSSFLKRKPMNLLFPVSITVSTVAIAVLVHKAMSIENDPFELLAFTFYI
                     VILALGLLEHWFLVLPIPAEALWNWSVKARKALSGAEHEELLPDDREAPQPAPTRVPA
                     VAGQDI"
     gene            complement(3794..4861)
                     /gene="acsF"
                     /locus_tag="VNR65_04970"
     CDS             complement(3794..4861)
                     /gene="acsF"
                     /locus_tag="VNR65_04970"
                     /EC_number="1.14.13.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR02029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048529 - magnesium-protoporphyrin IX
                     monomethyl ester (oxidative) cyclase activity [Evidence
                     IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     (oxidative) cyclase"
                     /protein_id="HWJ18065.1"
                     /translation="MTEATHPAEAINESTRHAQKDTILTPRFYTTNFDEIDRIDVTSV
                     RQEWDELIAEMKSDPNRTHFIRNEEFDVDTSALPEELRKEFEDFLISSVTAEFSGCVL
                     YAEMKKRAKNPDMKELFGLMARDEARHAGFINETLKDMGIGVDLGFLTKAKKYTFFQP
                     KFIYYATYLSEKIGYARYISIYRQFERHPELRFHPIFKWFENWCNDEFRHGEAFALLM
                     RANPHLLSGLNKYWVRFFVLAVFATMYVRDHSRPAFHKALQMDPTDYDFQVFRITSQI
                     SQQVFPVKLDLDNPALHRGFDRLYQLSLKREAAKAAGGISGLIKNMGYSAAAAVTFAR
                     LYILPAARNELPDRVRLAPAW"
     gene            complement(4858..5166)
                     /locus_tag="VNR65_04975"
     CDS             complement(4858..5166)
                     /locus_tag="VNR65_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18066.1"
                     /translation="MISIIDWLGGHETFEVPCTIEVEHTADSLHAYVELDGEVDIGPG
                     DQVIVQGAPIKPAFGEKIVERRQATVKRANWFDRMTARVAGLELTELYEVSFTPRRKL
                     "
     gene            complement(5163..5609)
                     /gene="puhC"
                     /locus_tag="VNR65_04980"
     CDS             complement(5163..5609)
                     /gene="puhC"
                     /locus_tag="VNR65_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic complex assembly protein PuhC"
                     /protein_id="HWJ18067.1"
                     /translation="MVQGIKKPALLAAAAIFAVLSLLVSMNLIGTQTVDLSPQRTVVL
                     ARPLTFRDAAGGAIAVYDQGASEPFAVLPREGNTFMASALRLLGQNRELRTKAGREAP
                     FTLTLWSDGKLSLSDTATGDTLELAAYGPTNAKTFAQLLPTAGKAQ"
     gene            complement(5630..>6186)
                     /gene="puhB"
                     /locus_tag="VNR65_04985"
     CDS             complement(5630..>6186)
                     /gene="puhB"
                     /locus_tag="VNR65_04985"
                     /inference="COORDINATES: protein motif:HMM:NF040894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="photosynthetic complex putative assembly protein
                     PuhB"
                     /protein_id="HWJ18068.1"
                     /translation="ITIYFSVLMLWPIAVAYSEGKPIGNALEIDIWLAALLGIVMAIA
                     LTFAFLAARTTIYTVTTRRVAIRYGIAFTKAVNVPLRHIKSVGMRMHANGTADVPLTL
                     TGPDKIAYLILWPHARPWCFSSPEPMLRAISEPQQVADALKTALAPFVKDAAEAASKS
                     SQETEIAPHAEDKNELGQAATEAA"
BASE COUNT         1333 a         1707 c         1718 g         1428 t
ORIGIN      
        1 atcggcctgg ggagctgtcg ccgcgcgcca agtccgtgcg gccttacgaa cctaatcagt
       61 taaattcatc cgtttgacag catcgatgct cacaatgcac tgcttttgta tatcatcaaa
      121 ttgggagctt tacgagagat agacgcaagt gggtgtcacg tttgcacctt ctcggcagga
      181 ttcttgtcgc ttatgtgggt cgacattaaa cctgactggt gagcacaaaa taaaggcttc
      241 agccctgcgc aatgaatttg gttccggcag cttattaatt agaatctctg ataattttaa
      301 caatcacata caattgaccc aaagcgtaag tcgaaggttt tcatttcacc gcgcgaattt
      361 gtagcgtttg caatcctacg cggacactac ccgctgaccg tgagtttgat cgttttcgcc
      421 aagccgctct cacgttcatc ggaaagggga gaagatccca gatttgtatt caataataag
      481 cggtatctaa ttggccaagc ctcaaatcgg gcttgtcccg tccgtattcg gcgccgcgca
      541 ccgctgagac gaaggatggc catccttcgt ctcaggccta ctctccagcg attcgtttga
      601 atgccaagat caggcgccat caacatacaa tcccggataa ggttcggccc ttccgggatg
      661 acgccctcgg cgaatcgttg gtgcgcccag caagcattag tttaccctac ctccagcacg
      721 atcttgccca tatgcgcgtt cgattccatg cggcggtggg cgtcggccgc ctgatcgaga
      781 gggaaggttt tgtcgatcac gatcttgacc gctccgcttt cgagaagtgg ccacaccttt
      841 ttctgaagtt ccttcgcgat cctggcctta aattcgagtt ttcgtggccg cagagtcgat
      901 cctgtgagcg ccagacgctt cagcatcagc ggcatgaggt ccaactggac gcgggcgcct
      961 ttcaggaacg cgatctgcac gatgcggcca tcttcggccg cgcaggcaat atcgtcctga
     1021 acgtaggcgc cgccgaccat gtcgaggatc acatccacgc cccgcttgca ggtcaggtcc
     1081 ttgatttcct tggcgaagga ttgcgtcttg tagttgatgg cgcgttcagc gccgagcttc
     1141 tcgcaatagg cgcatttttc gtcgctgccc gccgtcgcga agacgcgcgc accgaaatgc
     1201 gctgcaagct ggatcgcgct tgagccgatg ccgctcgatc caccgtgaac gaggaagctt
     1261 tcgcctgcct tcaacgcccc gcgctcgaag acgttatgcc agacggtcat gaaggtctcc
     1321 gggatacacg ccgcatcggc tatgctaagg ccctttggca ctctcatgca ggaaccctca
     1381 tgcgcgagcg cgtattccgc atagccgccg ccagcggtaa gagccgttac cttttcgccg
     1441 acgcgccatt gggacacgcc ttctccgaga gtcgcgattt cgccggcgac ctccagacca
     1501 ggaatgttcg agacaccagg cggcggtgga taaaggccca tgcgctggag aacgtcgggg
     1561 cgattaacac ctgcggcggc aacttttatc aaaacctctc cagcaagtgg ttctggcgtc
     1621 gcgagatcct gacatgtaag gacttctggt ccgcctggct ctcggatgac gattgttttc
     1681 atggcgtttc ctccagccta tcgtgccaag cccgattgca agacatttac cccaaattaa
     1741 caggctcttg ctattattca taaacgtaag ctgggtcgac ccggaaacac ctccaaaact
     1801 tttcagccgt gtccttgaca cggctttttt gttagctctt tttttgccgc gcccttgata
     1861 cggctttttt tttagctaat gtggcccaag cgcgtttcct gtcttcactc tttgaaaggc
     1921 agttgcggtt agatgtttgt tgtgtccggc gattaagcgc cggtcgttgt gcgtatggcg
     1981 gatcctatga agagctcctg cgtttcccag aaattccaga atactgctct tttgtcgttt
     2041 ggcgaccgtt tcacgacgtc gcctcagttc atgaaactat ttcgggaagg catggacctc
     2101 gtcgaggaaa ctgccgaata tctcgatggc gcggggagag cggaatctaa acagctggcg
     2161 ccccctgcaa gccttgctta tacgtcagag agcatcaggc taacgacgcg tcttacccaa
     2221 cttgcctcgt ggctgcttgt ccggcgggct atagcctctg gtgaaataac cgctgcgcaa
     2281 gcgcacacgc accgacaccg tgttactctt tcgccgcaga gcgctgtcca ccccgaaggc
     2341 tttgacgctc tgccggaatc attcaagaga ttgattgccg agagcttccg cctgcacgac
     2401 cggatcacaa ggctggagcg catccttggc gatagcccaa gcagagcgga taagtcccct
     2461 tcgccaatcg acgggcatat tgagcgcctc agaatggagt ttccggccgc gtgaacgcgc
     2521 gctttatttc ttgaaaaatg cgctcgcagc gtccttgtgc gcgtccttct gcttgatcac
     2581 cgtctctgcg cgcaaaatct ccgctttgag aagctcgacg tacgcttgca agtcgaccac
     2641 ggaataaacc gtaaggtcct ttggttttga aggctgcttg cgcggctcaa gatcgtggat
     2701 gtccattctt tgctccttcc gattcagcaa cgacacggga taccttagcg cgaagcgaac
     2761 gcgcttgcac ccggtcgcgg aggccggagg ctcggcggga gcgctatcat gcgggttgta
     2821 gaatcggcag cggcctcgct agaggccgac ctaaccattt catttttggc gagatgccga
     2881 ggagtgccga ccaagtggtt gtgcgaactc gaacaacggc tcgatttaga tgtcctgccc
     2941 ggcaacagcg ggaacacggg tgggcgctgg ttgaggtgcc tcacggtcat ccggcagtag
     3001 ttcctcgtgt tccgctcctg acagcgcctt tctggccttg acgctccagt tccaaagggc
     3061 ttctgcggga atagggagca ccaggaacca gtgttccaaa agccccaagg cgaggatcac
     3121 gatatagaag gtgaaggcga gcaactcgaa cgggtcattc tcaatcgaca tggccttgtg
     3181 cacgaggacg gcgatggcga ctgtggagac ggtaatcgac actggaaaca gcaggttcat
     3241 cggcttcctt ttcagaaagc tcgacaggta gcgcaactgc tccgggaaaa agtggtcatt
     3301 cagatttctc acacccagca ccgcatttat ctttgcgctc gaatgcatga gccagatgat
     3361 tgtatatgtc caagagccga cgtgattttt gccggtcatc gtcgagaagc agatcacaac
     3421 cccaagcagg gccgcagtga tctcgtgata catacttgtt tcgacggcat gccaaaaatg
     3481 acgggaacct ttgcaagatg acgggcaggg ggttgttcga ggaccggtta tcatgcccgt
     3541 gtagaatgta aactgggtcc acgcccaggc cagcagacca aaggtgaaac tcaaataggc
     3601 tgatacaata ctgtcgttct gactggttaa cgtcaggccg tagatcgacg cgacaaggac
     3661 gaatgtcgcg ccgaggaaac tccacttata cgtgtattta ggtagattgt tgaggtaaat
     3721 gacggctccc gtgctgaacc accaaacgaa gagaacgaac agaaccggga gcacgtatgt
     3781 aatcacctca tcctcaccat gctggggcca accgcacgcg gtcgggcagt tcattgcgcg
     3841 cggctggtag tatgtaaagc cgagcgaatg ttactgctgc cgcagcggaa taacccatgt
     3901 ttttgataag tcccgatatt ccccctgccg ccttggcggc ctcgcgcttt agggacagtt
     3961 ggtacagacg atcgaacccg cgatggagcg ctggattgtc caggtcgagc ttgaccggaa
     4021 agacttgctg ggagatctgg cttgtgatgc ggaagacctg gaagtcgtag tccgtgggat
     4081 ccatctggag cgctttgtgg aaagctggac gcgaatgatc acggacatac atggttgcga
     4141 aaaccgcgag cacgaagaag cgaacccaat atttgttgag cccgctcaaa aggtgcggat
     4201 tggcgcgcat cagcagcgcg aaagcctcgc catgacgaaa ctcgtcgtta caccaattct
     4261 cgaaccactt gaagatcggg tgaaagcgaa gctcggggtg cctttcgaac tggcgataaa
     4321 tcgaaatata gcgggcgtag ccaatctttt ccgaaagata agtagcgtaa tagatgaatt
     4381 tcggctggaa gaacgtgtat ttcttggcct tcgtcaagaa gccgagatcg acgccgatgc
     4441 ccatatcctt gagcgtttcg ttaatgaagc cagcatgccg tgcctcatca cgagccatga
     4501 gcccgaacag ctctttcata tctggatttt tggctcgctt cttcatctcg gcgtatagca
     4561 cgcaacctga aaactctgcg gttacagaac tgatcaggaa atcttcaaac tccttcctta
     4621 gttcctcggg taacgcagac gtgtcgacat cgaattcttc gttccggata aagtgagtgc
     4681 ggtttggatc gctcttcatc tcggcaataa gctcatccca ctcctggcga acggatgtca
     4741 catcgatccg gtcgatctca tcgaaattag tcgtgtagaa ccgtggcgtt aagatggtat
     4801 ccttttgcgc gtggcgggtt gactcgttaa tcgcctcggc tggatgagtt gcctcggtca
     4861 tagtttcctc ctgggggtaa aactcacttc gtagagctcc gtaagctcga gtccggccac
     4921 acgcgccgtc atccggtcga accaattcgc gcgcttcacg gtcgcctgcc gtcgctcaac
     4981 gattttctct ccaaacgctg gcttgatggg agccccttgg acaatgacct ggtcgcccgg
     5041 gccgatgtcc acctctccat caagttcaac atacgcatgc aggctatccg ccgtgtgctc
     5101 cacctcgatg gtgcaaggca cctcgaaggt ttcgtgcccc cccagccaat cgattatgga
     5161 tatcattggg ccttgcctgc agtgggtagg agttgggcga aggtttttgc atttgtggga
     5221 ccgtaggccg ccaactcaag cgtgtctcca gtggccgtat cggacagaga gagcttgcca
     5281 tcgctccaga gggtgagcgt gaagggcgcc tcccgtccgg cctttgtgcg cagttcccgg
     5341 ttttgcccta gaagacgcag cgcagaggcc atgaaagtgt tcccttcgcg tggcagtacg
     5401 gcgaagggct cgctggcacc ttggtcgtat acggcaatag cgccgcccgc cgcatctcgg
     5461 aaggtcagcg ggcgcgcgag gacaactgtc cgttgcggcg aaaggtcgac agtttgcgtg
     5521 ccgataagat tcatggaaac cagcagcgac agcacggcaa atatcgctgc ggctgccagc
     5581 agcgctggtt tcttgatgcc ttgtaccatt atgggccgtt tccgaatccc taagcggctt
     5641 cagtggccgc ctgacctaat tcgttcttat cttcagcgtg cggcgcgatt tcagtttctt
     5701 gggaactttt gctggcggct tcagctgcgt ctttcacaaa aggagcaagg gcagtcttta
     5761 gagcatctgc cacctgttga ggttcggaga tggctcgcag cataggctcc ggcgacgaga
     5821 agcaccaagg ccgggcatgt ggccacagga tgaggtaggc tatcttgtcc ggaccggtta
     5881 aggtcagcgg aacatcggcc gtgccgttgg cgtgcatcct cattccgaca gacttgatgt
     5941 gcctaagcgg cacattgacg gccttcgtga aggcgatgcc gtaacgaatc gccacacgcc
     6001 gcgtcgtgac ggtgtatatg gttgtgcgcg cggcgagaaa ggcgaaagtc aaggcaatgg
     6061 ccatgacgat cccgagcaga gcagccagcc agatgtcgat ctccagtgcg tttcctattg
     6121 gtttaccttc cgagtaggca acagcgatag gccagagcat cagcaccgag aagtagatcg
     6181 tgatgg
//
LOCUS       DATMGX010000307         4176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_297422, whole genome shotgun sequence.
ACCESSION   DATMGX010000307 DATMGX010000000
VERSION     DATMGX010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297422"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..341)
                     /locus_tag="VNR65_04990"
     CDS             complement(57..341)
                     /locus_tag="VNR65_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HWJ18069.1"
                     /translation="MPGDLKLAVCVFMNGDEKNARLLLDEKVQMRDLERDMTANHLRR
                     LRERCPESLETSSLHIDIARDLKRITAHFASVAYPILEEKGVLRKTRLIE"
     gene            595..1092
                     /locus_tag="VNR65_04995"
     CDS             595..1092
                     /locus_tag="VNR65_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWJ18070.1"
                     /translation="MAKLEYRFLMGEKNNTEDTMVARRQVAALPLRQAKDGALEILLV
                     TSRDTGRWIIPKGWTSKRIKDCKAAAREAREEAGVKGKILRDAIGTYRYIKREVGHGA
                     LIEVRVFLLKVSKRCKRWPEKRERRRAWFDLKVAASKVSDPELSTLIGTLQEGNGGGG
                     TSGQT"
     gene            complement(1329..3548)
                     /locus_tag="VNR65_05000"
     CDS             complement(1329..3548)
                     /locus_tag="VNR65_05000"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWJ18071.1"
                     /translation="MTIYIGTAIPRIDGKAKVTGAAKYAGEFNRPGLAYEAVMTSTIA
                     KGKITQIDASEAAAVEGVLSVLTHENRPPMAEGDSAYKDDVAPEGSPFRPLYNENVLF
                     SDQPIALVLAATWEAARFAASLVRVDYQEEKPTTDLFQQRESAFTLAGEAFAMSLAHP
                     RGDAAHAFAASEVRHAADYFVPVEHPNPMELYASTVVWEGGGKLTVYDKTQGVQNVQR
                     YLCGVFGAKSEDIRVLSPFVGGAFGSGLRPQYEAVLAVLGAQALQRSVRLVLTRHQMY
                     TLGYRPGTIEHLALAAKADGTLEATSHEAIAMTSQYEDFARNDTTWSAALYKCPNAKY
                     EHKLARLDVPTPCDTRAPGAASGVYGLECAMDELAVALKIDPLEFRLKNYSDRDQNED
                     LPYTSKALRECYAQGAEAFGWQKRNPEPRSMSDGKELVGWGMASGVWEALQMKASSRI
                     TLTSNGHAEVACATSDIGTGTYTIMAQVAADMLGLPIENISIKLGDSTLPQSPVEGGS
                     WIAASVSHAIAEAAHAVREELLSHAKKAANSPLAGLTAGEVTLSGGHIVSSGEPYRSV
                     SFADAMRHGGVDRCAVERSTDFKDNRQYARNVHSAIFAEVKVDEELGVIRVARVVSAV
                     AAGRILNTKTAHSQIMGGVVWGIGMALHEETLMDHRFGRFMNANLGEYHVPVNADIHD
                     IEVIFVDEPDAIINPLGIKGVGEIGIVGVAAAVANAVYHATGKRVRDLPITLDKLQR"
     gene            complement(3554..>4176)
                     /locus_tag="VNR65_05005"
     CDS             complement(3554..>4176)
                     /locus_tag="VNR65_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HWJ18072.1"
                     /translation="THGSGCSAIHGLNRIHAILGASDACVATHPSDMCVGLAALGATV
                     IAVGPGGERRIAFSDFHRLPGNTPDRDTNLNPDELITAIELPAKGFSRNYTYLKIRDR
                     LSYAFALVSVAAALEIDGGVIKEARLALGGVAHKPWRDEKAEAALAGQAPDEGAFKQA
                     ADIVLRDAKGLAHNAFKIDLARRTIVRALTQAANGTPQIQAFKTIR"
BASE COUNT          800 a         1252 c         1259 g          865 t
ORIGIN      
        1 attccagcta agggacgcaa gctcgccgcg cggcgagctt gcgtcaggga cacagatcac
       61 tcgatgagcc tcgtctttcg caaaacgcct ttctcttcca ggatgggata ggccaccgaa
      121 gcaaaatgtg ccgtgatccg cttcaagtcg cgggcaatgt cgatatgaag gctgcttgtt
      181 tcgaggcttt ccgggcaccg ctccctcaat cggcgcagat ggtttgccgt catgtcgcgc
      241 tccagatcac gcatctgaac cttctcatcg agaagcagcc tcgcgttctt ctcatcgcca
      301 ttcataaaga cgcacacggc aagctttaga tccccgggca cgggctatca tggccgcgat
      361 tttggcgtgg ccctcggctg agaaagcaag cccgtatttg atcttcttcg cgatgatgtc
      421 ccgaaacttt tgtcggcaat gtcgcccgca tgttcgagtt tgataacgaa gtcgaggata
      481 tcccgtgcca caatcacctg atcagctctc tcaagcgcga tagtgttgga aaagtcttca
      541 atttattttt agggccgcca tattgaacac ccgatcatac gaaatgcttc tcttatggcg
      601 aagttggagt atcgtttttt gatgggggag aagaacaaca cggaggacac gatggtagct
      661 cggaggcaag tggcggcctt gccgctgcgc caagccaagg atggcgctct cgaaatcttg
      721 ctcgtcacat ccagggacac tggccgctgg atcatcccca aaggctggac gtcgaagcgc
      781 atcaaggatt gtaaggcggc ggcccgggag gcgagggagg aagccggcgt taaaggcaag
      841 atcttgcgcg atgctatcgg tacttatcga tatatcaagc gggaagtggg ccatggcgct
      901 cttatcgagg tcagggtctt tctcctcaag gtcagcaagc gctgcaagcg ctggcccgaa
      961 aagcgcgagc ggcggcgagc ttggttcgat ttgaaggttg ccgcaagcaa agtgagcgat
     1021 cccgagcttt ccactctcat cggcacgctg caggagggga acggcggcgg cggcacctca
     1081 ggccagactt agagatgcag cgccgggcat caggtcctta gggcttgcgc agccgctggc
     1141 ctgggcttcg ggctactatt tgctggcaat acaggcggag ccgatagccc gcgataggca
     1201 tgtgcctacg accgtggtgt tcgcggcctt ctcggctgcg cgataattgg cccgcatgtc
     1261 agccccacct tttaccgcag ccagggattc tcacggcaac cgtccaaacg atcgcggttc
     1321 cccgcatttc atcgttgcag cttgtccaag gtgatcggca gatcgcgcac gcgctttcct
     1381 gtggcgtgat agaccgcgtt tgcaacggcc gccgccacgc cgacaatccc gatctcgccc
     1441 acgcccttga tgccaagcgg gttgatgatt gcatcgggct catccacgaa gatcacctcg
     1501 atgtcgtgga tgtcggcatt cacgggcacg tggtactcgc cgagattggc attcatgaag
     1561 cggccgaacc gatgatccat cagagtttct tcgtgcaggg ccatcccgat gccccagacc
     1621 accccgccca ttatctggct gtgcgcggtc ttggtgttga ggatgcgccc agcggcgaca
     1681 gcactgacga cgcgagcgac gcgaatgacg ccaagctcct cgtcgacctt cacctcggcg
     1741 aagatcgcgg aatgcacatt gcgggcgtac tggcgattgt ctttgaaatc ggtggagcgc
     1801 tccaccgcgc aacgatccac gccgccatgc cgcatcgcgt ccgcgaatga cacggaacgg
     1861 tagggttctc cgctactgac gatatgaccg cccgaaagag tgacctcgcc tgcggtcaag
     1921 ccggcgagcg gtgaattcgc agccttcttg gcgtggctca gcaactcctc gcgaacggcg
     1981 tgagcggctt ccgcgatggc atgcgagacg gacgccgcaa tccacgatcc accctccacg
     2041 ggagattgcg gcagcgttga atcgccgagc ttgatgctga tgttttcgat gggaaggccg
     2101 agcatgtctg ccgcgacctg cgccatgatt gtgtaagtac ccgtgccaat atcggatgtg
     2161 gcgcaagcca cctcggcgtg gccgttcgag gtgagcgtga tgcgcgaact ggccttcatc
     2221 tgcagtgctt cccagacgcc cgatgccatg ccccagccga caagctcctt cccatcgctc
     2281 atggagcgcg gttccggatt gcgcttctgc cagccgaacg cctcagcgcc ttgggcgtag
     2341 cattcgcgca gagccttgct ggtatagggt aggtcttcgt tctggtcgcg atccgagtag
     2401 ttcttgagcc ggaactcaag cggatcgatt tttaacgcca cggcaagttc gtccatggcg
     2461 cactccaggc cataaacgcc cgaggctgct ccaggcgcac gcgtatcgca tggcgtggga
     2521 acatccaatc gcgccagctt gtgctcgtac ttcgcgttcg ggcacttata gagggcggcg
     2581 gaccaagtgg tatcgttacg ggcgaagtcc tcatattgcg aggtcatcgc aatcgcttcg
     2641 tgactggtcg cttcaagcgt gccgtcggcc ttcgcggcaa gtgccagatg ctcgatcgtc
     2701 ccgggccggt agcccagcgt gtacatctga tggcgcgtca gcacgagcct cacggatcgt
     2761 tgcaaagcct gcgctccgag tacggccagc acggcttcat attgcgggcg cagccccgat
     2821 ccaaaggctc cgccgacgaa gggcgaaagc acacgaatgt cctcggattt cgcgccgaac
     2881 accccgcaca gatagcgctg cacgttctgg acgccctggg ttttgtcgta cacggtcagc
     2941 tttccgccgc cctcccagac cacggtcgag gcatagagct ccatcgggtt ggggtgctct
     3001 acgggcacga aataatctgc cgcgtgccgc acttccgaag ccgcgaaagc atgagccgca
     3061 tcgcctcgcg ggtgtgcgag cgacatggcg aaagcctcgc ccgccagcgt gaacgcgctt
     3121 tcgcgttgct gaaacaagtc ggtggtgggc ttctcctcct gatagtcgac gcggacaagc
     3181 gatgctgcga agcgagccgc ctcccaggtg gccgcaagca cgagtgcgat gggctggtcg
     3241 ctaaagagga cattttcatt gtaaagaggc cggaacggcg atccttccgg agcgacgtcg
     3301 tccttgtagg cgctatcgcc ttcggccatc ggcggccggt tttcgtgggt cagcaccgaa
     3361 agaacgcctt cgaccgccgc tgcctcgctg gcatcgattt gggtgatctt gcccttggcg
     3421 atcgttgacg tcataacggc ttcgtaggcg aggcccggac gattgaactc gccggcatat
     3481 ttcgctgcgc ctgtgacttt cgcctttcca tcaatccgag ggatggccgt gccgatatag
     3541 attgtcattg cgctcaccgg attgtcttga aggcctgtat ttgcggagtt ccgttcgccg
     3601 cttgtgtcag cgcgcgcaca atggtgcgcc gggcaaggtc tatcttgaag gcgttgtgcg
     3661 cgagaccttt ggcatcccgc agaacgatgt ccgccgcctg tttgaaagcg ccttcatcgg
     3721 gggcttgccc ggctagcgcc gcctctgcct tttcatctcg ccatggcttg tgagcgacgc
     3781 cgccgagagc aagacgagct tctttgatca ctccgccgtc gatttcgagg gccgccgcga
     3841 ccgacaccag cgcgaacgca taagaaaggc ggtcgcgtat cttaagatag gtatagttcc
     3901 tgctgaagcc cttcgccgga agctcaatcg cggtgataag ttcatctgga ttgaggttcg
     3961 tgtcgcgatc cggtgtattg cccggcaaac ggtgaaagtc ggaaaaagca atccgtcgtt
     4021 ctccaccggg accgacagcg ataaccgtcg cgccgagtgc cgccagccca acgcacatgt
     4081 ctgaaggatg ggtcgcgacg caagcatcgc tcgcgccgag gatcgcgtgg atgcggttga
     4141 gaccgtggat cgccgagcag ccgcttccgt gagtgc
//
LOCUS       DATMGX010000308         2908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_80910, whole genome shotgun sequence.
ACCESSION   DATMGX010000308 DATMGX010000000
VERSION     DATMGX010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80910"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VNR65_05010"
     CDS             <1..593
                     /locus_tag="VNR65_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWJ18073.1"
                     /translation="LDLDYAGSTGYGRAYRDRLNGNWGVADVEDTISAARFAASEGLA
                     NDQAIFVTGGSAGGYTVLAAIASANVFRGVASYYGICDLVALQRTTHKFEQGYQSTLL
                     GGSLDDHEALYRERSAIHHIDAIKTPLILFQGADDQVVPKEQSIMIAEALRSKGVAVE
                     YHEFAGEGHGFRNAETICACLERELGFYRMLMEEGS"
     gene            complement(606..1118)
                     /locus_tag="VNR65_05015"
     CDS             complement(606..1118)
                     /locus_tag="VNR65_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18074.1"
                     /translation="MALPLTGLVLFFEMTQPAKVINAGLAAAQMANGPSEVISDGSAK
                     PWPLRGYTSIARFEGVSAQRRVDKAEYAEPILTTSDARRVFASAPVGSLKPGFQAEFL
                     SRDRHRIALRIVSREPIFDRTIPDNARLMNFTEASTANRMTFSWGNWIYKAEIEDKGV
                     EPEVVVQKVL"
     gene            complement(1569..2612)
                     /gene="ruvB"
                     /locus_tag="VNR65_05020"
     CDS             complement(1569..2612)
                     /gene="ruvB"
                     /locus_tag="VNR65_05020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWJ18075.1"
                     /translation="MTGRLMDREKQDEDAGELSLRPQRLTEFIGQAQARANLKVFIDA
                     ARARGDALDHVLFAGPPGLGKTTLAQIVARELGVNFKLTSGPVIAKAGDLAALLTNLE
                     ERDVLFIDEIHRLNPAVEEILYPAMEDFQLDLIIGEGPAARSVRIDLAKFTLVGATTR
                     TGLLTTPLRDRFGIPIRLNFYTDDELEEIVKRGARVLHMRMTPDGAREVARRSRGTPR
                     VAGRLLRRVRDFAAVGGEKEVDRRVADSALGQLEVDSLGLDSMDHRYLRCIGENYGGG
                     PVGIETIAAALSEGKDAIEEVIEPYLLQQGFIGRTPRGRVLTLKAFRHLGLAAPQAVR
                     DSQLPLFEGPRDG"
BASE COUNT          627 a          823 c          836 g          622 t
ORIGIN      
        1 ggctcgatct cgattatgcg ggcagcaccg gctatgggcg ggcctatcgc gacaggctga
       61 acggcaattg gggtgtcgcg gatgtcgaag atacgatcag cgcggcgcga ttcgcggcgt
      121 cggaaggtct tgccaacgac caggcgattt tcgtgaccgg aggaagtgcg ggtggctata
      181 ccgtgctggc ggcgatcgcg tcggcgaatg tttttcgagg tgtagccagc tattatggga
      241 tttgcgatct cgtggcgctc cagcgaacca cccacaaatt cgagcaggga tatcagtcca
      301 cgctgcttgg cggatcgctt gacgatcatg aggcgctcta tcgggagcgt tcggccattc
      361 atcacatcga cgcgataaag acgcctctga tcttattcca gggggccgat gatcaggttg
      421 tgccgaaaga gcaatcgatc atgattgcag aggccctgcg ctcgaagggc gtcgcggtcg
      481 aatatcatga atttgcaggc gaagggcatg gtttccggaa cgcagaaacg atctgcgctt
      541 gcctcgaacg cgagctcggt ttctatcgga tgctcatgga agaaggttct tagaaaaaaa
      601 ggtccttaaa ggaccttttg aacgacgacc tcgggttcaa cgcccttgtc ctcgatctcc
      661 gctttgtaga tccagttgcc ccaggagaaa gtcatccggt tggctgttga agcctcggtg
      721 aagttcatga ggcgggcatt atcaggaatt gtccgatcga agattggctc gcggcttaca
      781 atacgaagcg cgatacggtg ccggtccctg gaaagaaatt cggcctggaa acccggcttg
      841 aggctcccga cgggagcgct tgcgaacact cgcctggcgt cgctcgtggt caggattggc
      901 tcagcgtact ctgccttgtc gacccggcgc tgcgcgctaa cgccttcgaa ccgcgcgatc
      961 gacgtatacc cgcgcagagg ccacggctta gcgctgccgt cgctgatgac ctcagatggg
     1021 ccgttcgcca tctgagccgc agccaatccg gcatttataa ccttagcggg ttgggtcatt
     1081 tcgaaaaaga gaacaagccc ggtaagcggg agggccatca gcgccccgca ccacatggca
     1141 cggcctaatg ttcccaaagt ggcccggctc ttttgcaagg taatgctcgt cgcgttgctc
     1201 atggcctgat aataagcaaa aaaggcttcc tgcgtctacg catctagaag tcgtcttgga
     1261 tcaaggcaat ttgatgatgg tacccgtgaa cgggtacgta tccctataac aaagatgtga
     1321 aggatgtgtt taacaaaact taacgaaatc gcctgacgta cgcaaaacca actcgtcagg
     1381 cgttccgttc catccatcgt ggcaattgag tccacgtgga ttggaacctc tgcagcactc
     1441 tgcttgggtt ttataaaagt gcattccagg cgtaatgtca tctgaaaaag cacacagccc
     1501 ctcaatctgc tgtgtgaaac ctgtgggaga atgttgactg tgtctctcac gctctcgtga
     1561 tgggcaaatc acccgtcccg agggccctca aaaaggggaa gctgactatc ccgtaccgct
     1621 tgtggagcgg cgaggccgag atgacgaaag gccttcagtg tgagtaccct cccgcgtggc
     1681 gtacggccga taaacccttg ttgtagaaga tagggttcga tcacttcttc gatcgcatcc
     1741 tttccttcgc tcagcgccgc cgcgatggtt tcaatcccta cggggccgcc gccgtaattt
     1801 tccccgatgc agcgcaaata gcgatgatcc atggagtcaa gccccagaga atcgacctca
     1861 agctgtccaa gggcggagtc ggcaaccctc cgatcgacct ctttttcgcc gccgaccgcg
     1921 gcgaaatctc gcacgcgccg gagcagccgt ccggcgacac gcggcgttcc gcggctgcgc
     1981 cgcgccacct cgcgggcgcc gtccggcgtc atccgcatgt gcagcacgcg cgcgccgcgc
     2041 ttcacgattt cttccagctc atcatcggta tagaagttga gccggatcgg aatgccgaag
     2101 cggtcccgca aaggcgtcgt tagaagccct gttcttgtcg tcgcgcccac aagggtaaat
     2161 ttcgcaagat cgattcgaac agaccgtgcc gctggcccct ccccgatgat gaggtcaagc
     2221 tggaaatcct ccatcgctgg ataaaggatt tcttccacgg ccggattgag ccgatggatt
     2281 tcatcaatga aaaggacgtc tcgctcctca aggttagtga gaagcgccgc aaggtcgccc
     2341 gccttcgcga tcacaggccc cgacgtcaac ttgaaattca cgccaagctc gcgcgcaaca
     2401 atctgtgcga gggtcgtttt ccccaagccc ggaggccccg cgaacagaac gtgatcgagc
     2461 gcgtctcccc gagcccgcgc cgcgtcaatg aagaccttga gatttgcgcg cgcctgggct
     2521 tgcccgatga actcggtgag gcgttgcggc cgcagcgaaa gctcgccagc gtcctcgtcc
     2581 tgtttttcgc gatccatgag acgtccggtc atgcgctcac gctaagcagg tttgcgcaag
     2641 cctgcaacat ttgctgatca aggtcggggg aagattcggc caaagcgccg cccgctgttt
     2701 ctcgcacact gcgatgccaa caaaacatct cgtatttata aagaagatgg tgtcgcgcct
     2761 tgataataag tgaaagaact tatgccatag ctatctgtgg cgcgtgtcat cgtgtgtata
     2821 taccgacacg taaggtacct tcgaaatcgt aagaaggccc tgtttagttc gaacttggga
     2881 agaggtgtta cggcgatcga gtgcggcc
//
LOCUS       DATMGX010000309         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_297419, whole genome shotgun sequence.
ACCESSION   DATMGX010000309 DATMGX010000000
VERSION     DATMGX010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297419"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2528
                     /gene="dnaE"
                     /locus_tag="VNR65_05025"
     CDS             <1..>2528
                     /gene="dnaE"
                     /locus_tag="VNR65_05025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWJ18076.1"
                     /translation="DRDGVYGAPRFHLAAKKLDIKAHIGAEIGSLLSPYPKTGEPAAS
                     RVPLLCASREGYQNLCRLVTRMKLRAPKYPAALPHRAKATLDDDLVLSSQAIASVDDL
                     SEYAAGLICLTGGDEGPLAHALGIGGMEAGRSTLKQLIAIYGRENVYVELQRHYNRAQ
                     EARNQSAIELARSLDLPLLATQGAQYAKPEERQILDVFTCIRNHRTLDTAGRLLARNS
                     ERYLKTPQEMLRLFADLPEATANTLELSSRLAFTLNDLGYQFPRYPVPEGETMNSFLR
                     TRTMEGARDRYLRPGQSELWQRAQRQIERELLLIKKLDLAGYFLIVWDIVRFCREQGI
                     LVQGRGSAANSAVCYSLGITAVDPVGMDLLFERFLSEERGEWPDIDLDLPSGDQRERA
                     IQYVYQRYGKLGAAMTANVITYRGRSAAREVGKALGFDGESLDRLSGLVSSWEYKDPD
                     DTLERQFRDAGFDLRHPRMRTFFELCVAVQDLPRHLGQHSGGMVICQGQLDSVVPLEP
                     ATMPGRVVVQWDKEDCADLGIVKVDLLGLGMMAVLEDSITLIRDHYEEEVDLGKLPPD
                     DPVVYSALQKADTVGMFQIESRAQMSCLPRLYPTRFYDIVVQVAIIRPGPIVGQMVNP
                     FLQRRQGRQAVVYPHPSLEPVLKRTLGVPLFQEQLLRMAMIAAGFSGGEAEELRRAFG
                     FKRSEMRMRDIEIKLRAGMERNGIPPEAQEQIILSITSFALYGFPESHAASFALIAYA
                     SAYLKCHYLAAFTAALLNNQPMGFYHPSTIVKDAQRHGLRVKPVDITRSEWPCTLEST
                     TMSDTVKEQKFPSGAKPSAEEATDTLPLGHWEGARLQPRRTQLT"
BASE COUNT          495 a          779 c          759 g          495 t
ORIGIN      
        1 tcgatcgcga tggagtgtac ggcgcgccgc gctttcatct ggcggcgaag aagctcgaca
       61 tcaaggcgca catcggagcg gagatcggtt ccctgctgtc cccctaccct aagaccggcg
      121 aacctgcggc ttcgcgcgtg ccattactgt gcgcctcgcg agaaggctac cagaaccttt
      181 gccgcctggt gacccgcatg aagttgcgtg cgcccaagta tccggctgcg ctgcctcacc
      241 gcgcgaaggc gaccttggat gacgacctgg ttcttagttc acaagccatc gcttccgtgg
      301 atgacctcag tgaatatgct gccgggctga tttgcctgac cggcggcgac gaaggcccgc
      361 tggcacatgc gctaggcatc ggcgggatgg aagctggccg cagcacactc aaacaattga
      421 tcgcgatcta cgggcgcgag aatgtttacg tcgaactgca acgccactac aaccgcgcgc
      481 aagaggcccg caaccagtcg gcgattgaac tagcgcgcag cctggacttg ccgttgctgg
      541 ccacgcaagg tgcgcaatat gccaagccgg aagagcgcca gatcctggac gtcttcacct
      601 gcattcgtaa ccatcgcacg ctcgataccg ccggccgcct gctggcacgc aacagcgagc
      661 gttacctcaa aactccccag gagatgctgc gtctcttcgc cgatctgccc gaagcaaccg
      721 caaatactct ggagctgtcg tcgcgtcttg cattcacgct gaacgatttg gggtatcagt
      781 tcccacgcta tcccgtgccc gaaggcgaaa ccatgaactc gtttctgcgg acgcgaacca
      841 tggaaggcgc ccgcgaccgc tatctgcgcc ccgggcaaag cgaattgtgg cagcgcgcgc
      901 agcgccagat cgagcgcgaa ctcctgctca tcaagaagct cgatctagcg ggttacttcc
      961 tcatcgtttg ggacattgtg cgcttctgcc gcgagcaggg catcctggtg cagggccgcg
     1021 gatcggcggc caacagcgcg gtttgttatt ccctcggcat caccgcagtc gatccggttg
     1081 gcatggacct gctattcgag cgctttctgt cggaggagcg cggcgaatgg cccgacatcg
     1141 atctcgacct tcccagtggc gatcagcgcg agcgcgccat acagtacgtc tatcagcgct
     1201 atggcaaact cggcgctgcc atgacggcca acgtcattac ctatcgcgga cgttcggcgg
     1261 cgcgcgaagt tggcaaggcg ctcggttttg atggcgaatc cctggaccgc ctctccgggc
     1321 tggtgagttc ctgggaatac aaggacccgg atgacacgct ggaacggcag tttcgcgatg
     1381 ccggcttcga cttgcggcat ccgcgcatgc gcacattttt tgagctttgc gtggcggtac
     1441 aggacctgcc ccggcatttg gggcagcact ccggtggcat ggtgatttgc cagggtcagc
     1501 ttgactctgt cgttccgctg gaacccgcaa ccatgcccgg ccgggtcgtg gtgcagtggg
     1561 acaaggaaga ttgcgccgac ctcggcatcg tcaaagtcga cctgctggga ctggggatga
     1621 tggccgtgct ggaagattcc atcactctga tccgcgatca ttacgaagaa gaggttgatc
     1681 tgggaaagtt gccgcctgac gatccagtag tttatagcgc gctacagaag gccgacacgg
     1741 tgggcatgtt ccagatcgag agccgggcgc agatgtcgtg cctgccgcgc ctgtatccca
     1801 cacgcttcta cgacattgtg gtacaagtcg ccatcatccg tccggggcct atcgtcggcc
     1861 agatggttaa cccttttctg caacgccgtc aggggcggca agccgtcgtt tatccgcatc
     1921 cctcgctgga gccggtcctg aaacgcacgc tgggggtgcc gctctttcag gagcaactgc
     1981 tgcgcatggc gatgattgcc gccgggttca gtggtggcga agctgaggaa ctgcgccgcg
     2041 ccttcggctt caagcgcagc gagatgcgca tgcgcgatat cgagatcaag ctgcgcgcgg
     2101 gcatggagcg caacggcatt ccgccggaag cgcaggagca gatcatcctt tcgattactt
     2161 cgttcgcgct ctatggattt cccgagtcgc acgccgccag cttcgcgctg atcgcctatg
     2221 ccagcgccta tctcaagtgc cattacctgg cggcttttac ggcagcgctg ctgaacaatc
     2281 agcccatggg gttctatcac ccttcaacca tcgtcaaaga cgcgcagcgg cacgggctgc
     2341 gagtgaagcc ggtagacatt acgcgatcgg agtggccatg cacgctggaa agtaccacaa
     2401 tgtcagatac agtcaaggag cagaaatttc cctcaggagc caaaccctct gctgaagagg
     2461 ctacagacac acttcctcta gggcattggg aaggggcacg gcttcagccg cgccgtacac
     2521 aactcaca
//
LOCUS       DATMGX010000310         2460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_42705, whole genome shotgun sequence.
ACCESSION   DATMGX010000310 DATMGX010000000
VERSION     DATMGX010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42705"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..773
                     /locus_tag="VNR65_05030"
     CDS             69..773
                     /locus_tag="VNR65_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18077.1"
                     /translation="MDTLIESIRAAFYKRGVPQSLYVDNGSIYSSKEIIQICARVGCL
                     LHHTPVRDGAAKGKVERFFRTTRDQFLSRSLDLSSLEALNRQFIAWVEEQYNAQTHSV
                     LGMSPLDRFALDRSRVRFLPPNEANDELFYVEEERHVRTDNTFSFKSLRFEAPRHLPD
                     RTIQIRFERRKPAGNVIVYYKGERMGVARLLDPVANDRKPETRSLQPEVRSQRLEVGS
                     QSPENKKPNLQTEAQP"
     gene            770..1570
                     /locus_tag="VNR65_05035"
     CDS             770..1570
                     /locus_tag="VNR65_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ18078.1"
                     /translation="MIRAHFGLQKIPFDTEALALLAHQQEIFDILKVHAQQGGLCLIL
                     GEPGTGKSILKQALVNQDQKRVITPVVNRTLHTYHNTLRILCAAFQIEFNGHDHHCER
                     LLAQEAFRVHRAGKMLVPIIDDAHLMSPDCLRKLRLLFEDFPRSHNLVLIAQPPLLQT
                     LALAQNEEIRSRVTYSVILPRLAPETIEAFVLSELDRAGLGHHTFTADALALIVRSGE
                     GLLRRTKNLCVSSLIEAVRDQTRIVDLKQVNRVLVQPHWRKDCDQPIA"
     gene            1551..1841
                     /locus_tag="VNR65_05040"
     CDS             1551..1841
                     /locus_tag="VNR65_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18079.1"
                     /translation="MTNPSHSTKVMPLPPWSREQIRDARKAPLAPLIAKRGLVLVELP
                     ADNFAVTAFPGLIVKDCYWRWPERDLAGNSIDFFVQVLGLSFHDAMKTITAS"
     gene            complement(2120..>2460)
                     /locus_tag="VNR65_05045"
     CDS             complement(2120..>2460)
                     /locus_tag="VNR65_05045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HWJ18080.1"
                     /translation="ALPLVLPADDDRAGQGVFSSRAPCRPACLGSCVVSLLRREENTL
                     VVRGLDALHGTPVLDVKPYVPQFDAFPDAAVPLHWAKVMQRPDDAARIARELHVTDFF
                     LIVKPAKLSS"
BASE COUNT          538 a          803 c          640 g          479 t
ORIGIN      
        1 ggctgatcgc gtttatcgac gacgcttccc gtgtgtgttg tcacggccag ttcttttgtg
       61 cggaaaacgt ggatacgctg atcgagtcca tccgcgccgc tttttataaa cgcggcgtgc
      121 cgcagagttt gtatgtcgat aatggttcaa tttattcctc aaaggagatc atccaaatct
      181 gcgcccgggt cggctgcctc ttgcaccaca cgccggtgcg cgacggcgcg gccaagggca
      241 aggttgagcg gttcttcaga acgacccgcg atcagttcct ctcgcgttcg ctcgatctct
      301 cgtcactgga ggcgctcaac cgccagttca tcgcttgggt tgaagaacag tataacgccc
      361 agacccattc cgtgctcggg atgtctccct tggatcggtt cgcgctggat cgcagccgcg
      421 tgcgtttcct cccgcccaac gaagccaacg acgagttgtt ctacgtcgag gaggaacgcc
      481 acgtgcgcac cgataatact ttttcgttta aatcccttcg ctttgaagcg ccgcgccatc
      541 tgcccgatcg cacgatccag atccgtttcg aacggcgcaa accagcgggc aacgtgatcg
      601 tttattacaa aggcgagcgc atgggcgtgg cccggctgct cgaccccgtc gccaatgacc
      661 gcaagcccga aaccagaagc ctgcagccag aggtcagaag tcagaggtta gaggtcggaa
      721 gtcagagtcc ggaaaacaaa aagccgaacc ttcagacgga ggcacagcca tgatccgtgc
      781 ccacttcggt cttcagaaaa tccccttcga cacggaagcg ctcgcgctgc tcgcgcacca
      841 gcaggagatc ttcgacatct taaaagtcca cgctcagcaa ggcggcctct gtctgatcct
      901 cggcgaaccc ggcacgggca agtccatcct caagcaagcc ctcgtgaatc aagatcagaa
      961 acgtgtcatc actccggtcg tcaaccgcac gctgcatact tatcacaaca ccctgcgcat
     1021 cctctgcgcg gccttccaga tcgagtttaa cggccacgac catcattgcg aacgcctgct
     1081 cgcgcaggaa gccttccgcg tccaccgcgc cggtaaaatg ctcgtcccga tcatcgacga
     1141 cgcccacctc atgtcgcccg actgcctgcg caaactgcgg ctcttgtttg aggattttcc
     1201 gaggtcgcat aacctcgtcc tcatcgccca gccgccgttg ctgcagacct tggcgttggc
     1261 tcaaaacgag gagatcagat cgcgagtcac ctactcggtg atcctgccgc gcctcgcgcc
     1321 cgaaacgatc gaagcgtttg ttttgagcga actcgaccgc gccggtttgg gccaccacac
     1381 cttcaccgcc gacgcgctcg ccctcatcgt gcgctccggc gaaggcctct tgcgcagaac
     1441 gaaaaacctc tgcgtgtcgt cgctgatcga ggccgtccgt gaccagaccc gcatcgtcga
     1501 tttgaaacag gtcaaccgcg tgctcgtgca acctcactgg agaaaagact gtgaccaacc
     1561 catcgcatag tactaaagtt atgccccttc cgccctggtc gcgcgaacag atccgtgacg
     1621 ccagaaaagc accactggcc ccgctcatcg ccaagcgggg tctcgtgctg gtcgaactgc
     1681 ccgccgacaa ctttgccgta accgccttcc ccggtctcat cgtcaaagac tgctattggc
     1741 gctggcccga gcgcgatttg gcaggcaact caatcgactt cttcgtgcag gtgctcggcc
     1801 tctcgttcca tgacgccatg aaaaccatca ctgcatcgta agtgtatcca gcatttggtg
     1861 acaaaccaat cgctgccgtc gtaggcgtac gatggctatg tggcccaaaa acgtacgacc
     1921 acagttcggc gcaaaatcat acgaacactc cggtcggaac gcgtacgatc aaaaatcggc
     1981 cgcgcacaaa atccacgctc tcatcgtatc cgtcgcatcc ccatttccag gcacctgctc
     2041 cgcctcatca caaatgcgac cccagcgcgg caatttggcc gcaatttacg accccatctc
     2101 ggctaaccac ccaaaacctc tacgaggaca atttggctgg ttttacgatc agaaaaaaat
     2161 cggtaacatg taattcgcga gcaatacggg ccgcatcgtc cgggcgctgc atgactttag
     2221 cccaatgtaa tggtacggca gcgtcaggaa acgcgtcgaa ctgcggcacg tagggcttca
     2281 catcgagcac gggcgtgccg tgcagcgcat cgagcccccg cacgacgagc gtattttcct
     2341 cacgccgaag cagcgaaaca acacaggagc ccaggcacgc cggacggcac ggagcccgcg
     2401 agctgaatac gccttggcca gcgcggtcat cgtcggcggg caagacgagc ggcaatgcat
//
LOCUS       DATMGX010000311         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_350919, whole genome shotgun sequence.
ACCESSION   DATMGX010000311 DATMGX010000000
VERSION     DATMGX010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350919"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..799)
                     /locus_tag="VNR65_05050"
     CDS             complement(167..799)
                     /locus_tag="VNR65_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ18081.1"
                     /translation="MQDQVNATPAPKRVPWNKGKLTGAKPPLRPKHVWLIRTKLQIEG
                     RARDLAMFNLAIDSKLRGCDVVAIRVEDVAAGGYTADRATVRQKKTGRPVRFELSEQT
                     RQAVDDYLKASNKRPGEFLFTGRRGPDRNMTTRQYARLVAEWIGSVGLDPRLFGTHSL
                     RRTKATLIYRHTGNLRAVQLLLGHTKIESTVRYLGIEVDDALAIAEQVDV"
     gene            1017..>2290
                     /locus_tag="VNR65_05055"
     CDS             1017..>2290
                     /locus_tag="VNR65_05055"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWJ18082.1"
                     /translation="MPISSRFVVNSTIFLLLVGFLALLAIVGMTIWLGENAQRYVDEA
                     SQVRDSRISAVELRSALQSAESSQRGFLVGGNEIYLAPYGNAKTQAQAQFERLKASLA
                     PSSQTDRMIKRLADVVTEKFDEMDRAIALKSDLHDADALVLFRSNRGKALMDEANVFL
                     SSIIRISDERLTTGIAEQRRNATWLRWVSVIGGLIIIIVVGGVTITIFRYTREIAQTR
                     DEGQVLNASLEQRVRSRTADLIAARDKAEVLLAEVNHRVANSLSMVAALVRLQSNGIQ
                     DQVAKDALNETQGRIDAISAVHKSLYSSGDARFVDLEQYLSGLLSNVETSMRNEGHGA
                     SLRYDLEPLKLKPDLSVNLGVIVTEWVTNAFKYAYPDRAGEVRVHLKRLSEGRAELVV
                     EDDGVGRGVEGAPKGSGLGTRIVNAMARTIGA"
BASE COUNT          454 a          651 c          696 g          489 t
ORIGIN      
        1 ctataagttg accgccgggc gttaccactt ggcaaagagc ttttacgcat caaggaacga
       61 ttgaaggtcg cttcaatcgg tgaaatcctt cggcgcttga tgtcgccctt tggcacaaac
      121 cggcggtcag aggttgggca gagcatgtcc gctctgcccg ggtatttcag acatcaactt
      181 gttccgctat tgccaaggca tcatcgactt cgatgccgag ataccggacc gtgctttcga
      241 tcttggtgtg tccgagcaag agctggacag ccctgagatt gcctgtatgg cgatagatca
      301 aggtagcttt tgtgcgtcgc agtgaatgcg tcccgaacag ccttggatcc agcccgacgc
      361 ttccgatcca ttccgctacg aggcgcgcat attggcgcgt tgtcatgttg cggtcgggac
      421 cacgacgacc agtgaacaag aactcgccgg gcctcttgtt ggaagccttc agatagtcat
      481 cgacggcttg gcgggtctgc tcgctcaatt caaatctgac cggccgcccg gttttctttt
      541 gccggacggt tgcgcgatcc gccgtgtaac cgccggcagc gacatcctcg acccggatag
      601 caacgacatc acacccacga agcttgctgt ctattgccaa attgaacatg gccaggtcgc
      661 gagcgcgacc ttcgatctgg agctttgtcc ggatcaacca aacgtgtttg ggtcgcagcg
      721 gtggctttgc cccggtcagt ttgcctttgt tccacggcac tcgcttgggc gcgggggtcg
      781 cattcacttg atcctgcatg gccatactcc tcggctcggt tacagaagcg ggaagcatga
      841 gccaccgggt taggcgttgg aatcaggtcg gttttctggc caaagcggac atcgcgtccg
      901 ccccggcggc aaacgacctg tagcgtttgg cccaacgcag aatggatgct gggcgaagtt
      961 cttttctgct acagtgatac cgacgggccg gtgcccgtga gaaattttag ccgcgtatgc
     1021 ccatatccag ccgttttgtt gtcaactcga ccatttttct gctgctggtc ggctttctgg
     1081 cgttgcttgc catcgtcggc atgacgatct ggctggggga aaacgcgcaa cgttacgtcg
     1141 acgaggcctc ccaggtacga gacagccgga tatcagccgt cgaactgcgc agcgcgttgc
     1201 aaagcgccga gtccagccag cgcggctttt tggtcggcgg caacgaaatt tatctggcgc
     1261 cttacggcaa cgccaagacg caggcccagg ctcagttcga acgtttgaag gcctcgcttg
     1321 ctccttcatc gcaaaccgac cgcatgatca agcgattggc cgacgtggtt accgaaaagt
     1381 tcgatgaaat ggaccgcgcg atcgctttaa agagcgatct tcacgatgcc gatgcgctgg
     1441 tcttgtttcg cagcaaccgc ggcaaggcgc tgatggacga ggcgaacgtc tttctgtcga
     1501 gcatcatccg gatatccgat gagcggctga cgaccggaat cgccgaacaa cgccgcaatg
     1561 cgacctggct ccgctgggtc tcggtgatcg gcggattaat catcatcatt gtcgttggcg
     1621 gcgtcacgat tacgatcttc cggtacacac gcgagattgc ccaaacccgc gatgaaggcc
     1681 aggtgttgaa cgcttccctt gaacagcgcg tcaggagccg aaccgcggat ttgatcgcgg
     1741 cgcgcgacaa ggccgaggtg ttgctggccg aggtaaatca ccgcgtggcg aacagccttt
     1801 caatggtggc cgcgctcgta cgtctgcagt ccaacgggat ccaggatcag gtcgcaaagg
     1861 atgccttgaa cgagacgcag ggacggatcg atgcgatatc ggcggtgcac aagtcgctgt
     1921 acagttcggg cgacgcgcgg ttcgtcgatc tcgaacagta tctttcggga cttctcagca
     1981 atgtcgaaac gtcgatgcgt aacgagggcc acggcgcctc gctgcgatac gacctggaac
     2041 cgctgaaatt gaagccggat ttaagcgtca atctcggcgt gatcgtgacc gagtgggtta
     2101 ccaatgcctt caaatatgcc tacccggatc gggccggcga ggtgcgtgtc catctgaagc
     2161 ggctttccga ggggcgcgcg gaactcgttg tcgaggacga cggcgtcgga cgcggcgtgg
     2221 agggcgcgcc caagggctcc ggacttggga cgcggatcgt caatgcgatg gcgcgaacca
     2281 tcggcgccga
//
LOCUS       DATMGX010000312         5379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_303196, whole genome shotgun sequence.
ACCESSION   DATMGX010000312 DATMGX010000000
VERSION     DATMGX010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303196"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VNR65_05060"
     CDS             complement(<1..759)
                     /locus_tag="VNR65_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18083.1"
                     /translation="MVFDSVEISTPAEESPAVSWPAIAAGAIAAAALTLVLLAFGAGM
                     GFSAVSPWGNSGISASTFQITTGLYLIVVALLASAMGGYVAGRLRTRWVGVHTHEVFF
                     RDTAHGFLAWAFATVIGAGFLAAAASNIAGGASPGLASAANVSATAGSSSPLDYYVDA
                     LLRPGSAATPNTTDPGARREIEGILTKGIADGEVAGPDRTYVAQVVAARTGLSQADAD
                     KRVSNVIDQAKTDLDTARKAAAKLSLWLTAALLTG"
     gene            complement(845..1018)
                     /locus_tag="VNR65_05065"
     CDS             complement(845..1018)
                     /locus_tag="VNR65_05065"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWJ18084.1"
                     /translation="MDKDRVIGSAKQIKATVKQVVGKAVGDAKLEAEGNADKIEGQVQ
                     NAVGGVKDTLKGK"
     gene            complement(1251..1865)
                     /locus_tag="VNR65_05070"
     CDS             complement(1251..1865)
                     /locus_tag="VNR65_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18085.1"
                     /translation="MRKSLYVGVAAVALAISASSGNAMSINIGAGLKPALDAADMIQK
                     AAVFIIEGNRYCFYFNGWHGAGWYRCGFAWRRNLGWGGEYGWQSWSYGPAERRYGHGG
                     RDRGTIEGPGKNVEGGTTIRRSPPAAGSKGGAEINAGGQMQKNTAPVGGGQMQKNAAP
                     AGGNQGGGEGHGQGGGQGGGQGGGQGGGPGGGPGGGQGGGGEKR"
     gene            complement(1947..2405)
                     /locus_tag="VNR65_05075"
     CDS             complement(1947..2405)
                     /locus_tag="VNR65_05075"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HWJ18086.1"
                     /translation="MRKTLILVTTAALLTGTAITGSAVARDRSDRTELTTSQITDQAA
                     ARAAQMKADLRLTAEQEKNWPAFETAVVDVWKKQAEQQIAWRNAHAKEQGSVDLIDEM
                     RKDADEQIDRSNARKKLADAAQPLYGSLDDQQKRRFSEALFRRDRDRHSY"
     gene            complement(2622..2873)
                     /locus_tag="VNR65_05080"
     CDS             complement(2622..2873)
                     /locus_tag="VNR65_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18087.1"
                     /translation="MKKILLATVAIVAFSAPSFAAETAKTVGQAPPSFYLAQDSATLK
                     CQIVETQPVAGGSMKVIGTAHPTKASAETALKADKTCAK"
     gene            complement(3120..3767)
                     /locus_tag="VNR65_05085"
     CDS             complement(3120..3767)
                     /locus_tag="VNR65_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18088.1"
                     /translation="MTGWRAILAALIGVIAGSVITAGVFLYTGAKVLQERTARELLSE
                     VVALVSLQEGWLLQGEGSQTAKLSDHPGLPEAQSGSGLWLLPVELRVILDEAKWNSPS
                     NPSYGFVQGRRVWIIRNETLPNAPLSYSGVVQDHFPALISSAGRQELCAWIERVQIAY
                     NGGTLTDRGLAPLRPYLVPLGQEDRVNAFRILLSQDARNFLAQIRARWDSTLFAD"
     gene            complement(3947..4222)
                     /locus_tag="VNR65_05090"
     CDS             complement(3947..4222)
                     /locus_tag="VNR65_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18089.1"
                     /translation="MGSAIKPFGISTKDLGMPAILAAIVLLLFALPSRAEDERVRIPD
                     GCREAADRAGLPLTLTHAEAARAIAYIRVMLSQDAAVARCRLALRGY"
     gene            4509..5057
                     /locus_tag="VNR65_05095"
     CDS             4509..5057
                     /locus_tag="VNR65_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18090.1"
                     /translation="MPGNRSRTAKASRVAVALILFAAFVLSACVQSEKPLLDGSKPLL
                     GAQFELNLYQDFIEGKALSVKTSVFRWNGTHYALVSGDSSGEKYFVIYPFGSNDLLIE
                     ATYENDNVYLLARKQAKGTYRILPIDQNDVDEATRIRTCVTRNLIICMIQTRQQLDTF
                     VRAAAAKTGGYITIGVISAAAR"
     gene            complement(5112..>5379)
                     /locus_tag="VNR65_05100"
     CDS             complement(5112..>5379)
                     /locus_tag="VNR65_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18091.1"
                     /translation="RNSLIRLEKLDFARKAAGRAGGRGPDCRRRCCPDAAWTRASPGY
                     GFGLPITRELAELYGGSLSLSRSALGGLKAELRLPRGAKGSSFA"
BASE COUNT         1065 a         1669 c         1539 g         1106 t
ORIGIN      
        1 gccggtaagc agagcggcgg tgagccacag agacaatttg gccgctgcct ttcgagcggt
       61 gtccaaatcg gtctttgcct ggtcgatgac gttcgaaacc cgcttgtcgg catccgcctg
      121 actaagcccc gtgcgggctg cgacgacttg cgccacatag gtgcggtctg gcccggcaac
      181 ctcgccgtca gcaatgccct tcgtcaggat accctcgatt tcgcggcgag caccaggatc
      241 ggtcgtattt ggggttgcgg cagagcccgg tcgcaacagc gcatctacat aatagtccaa
      301 agggctactg gagcccgcag ttgccgacac attggctgcc gatgcaagtc ccggtgatgc
      361 gccgccagcg atgttgctgg ccgccgcggc cagaaagccg gcgccgatca ctgtggcgaa
      421 tgcccacgcc aaaaagccat gagcagtatc acggaagaag acctcgtggg tatggacgcc
      481 gacccatctc gtacgcaacc ggccggcgac gtagcccccc atggctgagg cgagcagcgc
      541 gaccacgatc agatagagac ctgtggtaat ttggaatgtg ctcgccgata tcccggaatt
      601 gccccacggg gatacggccg agaaacccat tcctgcacca aatgcaagaa gaacgagggt
      661 tagggcggcg gccgcaattg caccggccgc aatcgctggc caagatacag cagggctctc
      721 ttcggcaggg gtggaaatct ctacagagtc aaaaaccatc gctgcctcca gatgaaaggc
      781 ccttgccatc tcggctatag ggagacccca agactaacgt gaagaataag ctcgcagagc
      841 tggcctattt tcctttgagc gtgtccttga cgccgccgac cgcgttctga acctgaccct
      901 caatcttgtc agcgttgcct tccgcctcca gcttggcgtc accgaccgcc ttgccgacca
      961 cctgcttgac tgtagccttg atctgcttcg ccgatccgat aacccggtcc ttgtccatag
     1021 tcatatcctc cgatgctcaa cgtaagttgc ggtgtgaact ggcggtcgtc gttgatttgc
     1081 atcaacgtta ttccctagat gactttcgtg atcccatagt ccatgagcgt tggcggacgc
     1141 ccgctttggc gtgcattgcg gactcaagtc ggacatcgcg caagattcaa aaagcaaaga
     1201 ggccgcatcg gcggcctctt tcttgtcgat ggtagcggat ggagcgggcg ttatcgcttc
     1261 tcgccaccgc cgccctggcc accaccgggg ccgccgcccg ggccaccgcc ttggccgcca
     1321 ccctgaccgc cgccttggcc accaccctga ccgtggcctt cgccgccacc ctggttgccg
     1381 ccagcgggcg cagcgttctt ctgcatctgg ccgccgccga caggcgcagt attcttctgc
     1441 atctgcccgc cagcgttgat ttctgcgccg cctttcgatc ccgctgccgg cgggctgcgg
     1501 cgaatggtgg tgccgccctc gacgttcttg cccgggcctt cgatcgttcc gcgatccctt
     1561 ccgccgtggc cgtaacgccg ctcggcaggt ccgtagctcc agctctgcca gccatattcg
     1621 ccgccccagc cgaggttgcg ccgccaagca aagccgcagc gataccagcc ggccccgtgc
     1681 cagccattga aatagaagca gtaccggttg ccttcgataa tgaagaccgc cgccttttgt
     1741 atcatgtcgg cggcatcgag cgccggtttc agtccggcgc cgatgttgat cgacatggca
     1801 ttcccgctac tcgcgctgat tgcgagcgcc acggcagcca cgccgacgta cagtgatttc
     1861 cgcattggac cctccagatt cgtgatctat ttgaatcttc caattgcgag cagccaggag
     1921 ggctgctcgc aattcaatct cgttgctcaa taggagtggc gatcacgatc cctgcggaag
     1981 agcgcctcgg aaaaccggcg cttctgctga tcatccaggc tcccataaag cggttgggcg
     2041 gcgtctgcga gcttcttccg tgcattcgat cggtcgatct gctcgtcagc atccttgcgc
     2101 atttcgtcga ttagatcgac gctgccctgt tccttggcgt gggcattgcg ccaggcgatc
     2161 tgttgttcgg cctgcttctt ccacacatca accacagcgg tctcaaaagc aggccaattc
     2221 ttctcctgct ctgcagtgag gcgcaggtca gccttcatct gcgccgctcg ggccgcggct
     2281 tgatccgtga tttggctcgt cgtcaattct gtacggtccg aacggtctcg cgcgaccgcc
     2341 gacccggtga ttgccgtacc ggtcaacaag gccgcagtcg tgacgagaat aagggtcttt
     2401 cgcatgacgt cctccttttg aggatattga gttgggaatg gtcgtgtttg ttgtggcacg
     2461 cagcgtaaag gcccagtgat cgattgggcc tttagttgaa ttatctttgt cggtggttgg
     2521 cggcgccctc ggtgcggtag tcggcagcgg cgggacgggt cggtagtttc gggactccag
     2581 gtaagctgat cgacccgccc cgtgcccgcg ccgtcacttc actacttggc gcaagttttg
     2641 tcagccttca aagccgtctc tgccgatgcc tttgtcgggt gagcggtgcc gatcaccttc
     2701 atgctgccgc cagcaacagg ctgggtttcg acgatttgac atttcaaggt cgcgctatcc
     2761 tgagccaggt agaacgatgg cggcgcctgg ccaacggtct tggccgtttc agccgcgaat
     2821 gagggtgcag agaatgcgac gatggcgacc gtcgctaaga gaattttctt catggtactc
     2881 accaattgtt gtgcgtccga tatcggacga agaacgaatg gagtcgatcg atgatggttc
     2941 ctgccatgac ggatttgttt ttgacgcgcc ggttgatata gatcaatgac aaccggtgat
     3001 gtctttcgcc tgaggaaatg gcccatgcac tctcaaggtc gctccacctg gcctgaccga
     3061 gcgttttttg aaagcgaaat cgcttcgtta ggtgcaaatg ctcacgatag ttgtgcgcgc
     3121 taatccgcga acaacgtgct gtcccagcgt gctcgaattt gagccaggaa atttcgagca
     3181 tcctgagaca acaggatacg aaatgcgttc acccgatcct cctgacccaa aggcaccaga
     3241 tagggcctga gcggtgcaag tccacgatcg gtcagcgtcc cgccattgta ggcaatctgc
     3301 acccgttcaa tccaggcaca caactcctgc cttcccgcag agctgatcag cgcgggaaaa
     3361 tggtcttgga cgacacccga gtacgagagc ggagcattgg gcagcgtttc atttctgata
     3421 atccagaccc tgcgcccttg tacgaagccg tagctcggat ttgacggaga attccatttg
     3481 gcctcgtcca gaattaccct gagctccacc ggcaaaagcc aaaggccgga gccgctttgt
     3541 gcttctggaa gtcccgggtg gtccgacaac tttgcagttt ggctgccttc gccctgaagc
     3601 agccagccct cctgcagcga cacgagagcg accacctcgg aaagcagttc ccgcgcagtc
     3661 ctttcctgga gaactttcgc tccagtataa aggaacacgc cagccgttat aacgctgcct
     3721 gcaatgacac caatcagagc ggcgaggatg gccctccaac ccgtcatttg atcctgtcgt
     3781 ccccaccgaa tgtattctat catgaataac accaacgcaa gatgcacaca atcgaaaggc
     3841 gattgcgaag ggcccgcact tcgacagagc ccattgccgc ttgttgaaca gcgccttcgc
     3901 acgcggccct gactcccggg ggcactgaaa ggatcggggc ccccgctcaa tagccgcgga
     3961 gcgccaggcg gcagcgcgcg accgccgcgt cctggctgag catgacacgg atgtaggcga
     4021 tggcgcgcgc ggcctcggca tgggtcagcg tcaaggggag tccggcccga tcggccgcct
     4081 ccctgcaccc atcggggatg cgcacgcgct cgtcctccgc gcgcgagggc agagcgaaca
     4141 gcagcagaac tatcgctgcg agtatggcag gcatcccaag gtctttagtc gagatcccaa
     4201 atggcttgat tgccgagccc aaggttgctc cttttcggcg gaaccggcaa ggcagagcgg
     4261 cagatgcaac gtcagtccga accggtctgc ctgtcacctt gatgatcacg cctgcgggct
     4321 ctcaaatctg ggcttccgga cgactcagaa aatggctacc tttcacgccg attggcagtg
     4381 atccggcgaa acccaactcg gcgacaaaag atgcgcccgc gacgaggccg ttccggggaa
     4441 gccaatttgt tccttggctt gcgtcgatcc gcaccagccc ctgtacgcgg aaagatgggg
     4501 agtacgacat gccaggcaac cgatcgcgaa cagcgaaggc atcgagggtc gctgttgcac
     4561 tgatcctgtt cgccgcattc gtgctctccg cctgcgtgca atccgagaag ccattactgg
     4621 acggatcgaa gccgctgctc ggagcgcaat tcgagcttaa tctttatcaa gacttcatcg
     4681 aaggaaaagc gctctccgtg aagacatcgg tcttccgctg gaacggcacc cactacgcgc
     4741 ttgtcagcgg cgattcctca ggtgagaaat actttgtaat ttaccccttc ggcagcaacg
     4801 atttgctgat cgaggccacc tatgaaaacg acaatgtgta tctgcttgcc cgcaagcagg
     4861 ccaaagggac ctatcgcatc ctgcccattg accagaacga cgtcgatgaa gccacgcgca
     4921 ttcggacatg tgtgacgaga aatctaataa tctgcatgat tcagacacgc cagcagcttg
     4981 acacttttgt ccgcgcagcg gctgccaaga ccggcggcta catcacgatc ggggtgattt
     5041 ccgctgcggc gaggtgaggc gagagagcga aaaatatctt ctgccggggg cgcgaactgc
     5101 ctgcccaaga ttcatgcgaa actgcttcct ttcgcgcccc gcggcagacg aagctcggct
     5161 ttcagtccgc cgagcgcgga tcgggagaga gaaagcgatc cgccgtacag ttcggcgagt
     5221 tcacgcgtga tcggcaggcc gaagccgtac cccggggacg ctctcgtcca ggcggcgtcc
     5281 gggcaacagc gcctccggca atcgggtccg cgtccccccg cgcgcccagc ggctttgcgc
     5341 gcaaaatcta atttctccag ccggatcaag ctgtttcta
//
LOCUS       DATMGX010000313         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_256805, whole genome shotgun sequence.
ACCESSION   DATMGX010000313 DATMGX010000000
VERSION     DATMGX010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256805"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..2026
                     /locus_tag="VNR65_05105"
     CDS             272..2026
                     /locus_tag="VNR65_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18092.1"
                     /translation="MQKKSSAGSSRNHPRIPPEHSGVQVNHCKSPGCMNYGIAAEPKS
                     VPGNNRYTLEGKRGIASCLCTSCGVEFPLKSNIGIVEEIERLASYLSPPTSLFCPNED
                     CANHSSRVPAGAPGAYASFGKTAIGNPRWRFNVCGKTFSQNLKATARQREPHKNKTIF
                     KLLVNKMPVRRIIEVTDIDPQTFYNRLGFLHRQCQLFAARRESALADLPIRRLYLGVD
                     RQDYLVNWLGRKDKRNTRLSAIAVVDNEYGYCFGAHLNFDPSLDRKAIQTEVEKSGDL
                     SKPYPHRRFARLWIDADHEKASAKSMIAKQRKLGLAMEIDETYSAALTRDDIESPDTP
                     SPATRLPEYGMQIHGEYTMYGCFFFLKRLLGNVQKWRFFLDQDPGLRAACLAAFRNEI
                     QSRTADAFYVGVAKDLTVDEKRQKYLEAKARFEKAQTAHPAMKPKEVKLMLIKERLAT
                     MTPHGKWEDRWLDHPFPTMGEPEKAVAYLTDFGDYDEDHRAWLYNKASLHGVNAFFNQ
                     VRRRLSFLERPIHSKTNKGRIWYGYSPYDPGNVIKVLDIMRTVHNYILTGKDGKTPAE
                     RLGLAQAPLDYEDIIYLS"
BASE COUNT          517 a          607 c          591 g          459 t
ORIGIN      
        1 tcacaggtaa gtagaaacca ttcattatca ataggttgac ggaattactt cccgccggaa
       61 atgtggcgtg gagggaagaa attctggcag gaattaattg cttgttgagc atgatggtga
      121 gcggaaataa ttccggcgcg ctcttgctaa ccaagatccg gtaaacgtgt gcccatcgtg
      181 gaaaattttg caagccgttg acttaatctg ttgatccgct acggttggcg cgcgtaaatt
      241 ggtgcgctga ctgatttttt gaatttggat catgcagaag aaatcttccg caggttcgtc
      301 ccggaaccat ccgcggatac cacccgagca cagcggcgtc caagtcaacc actgcaaatc
      361 tccgggctgt atgaactatg gcattgcggc agagccaaaa tcggttcctg gcaacaatcg
      421 ctacacgctg gagggcaaga gaggcatagc ctcctgcctg tgtacgtcct gcggcgtgga
      481 attccctctc aagagcaaca tcggcatcgt ggaagaaatc gagcggctgg cgtcctactt
      541 atcgcctcct acttccttgt tctgcccaaa cgaggattgc gccaatcact ccagccgggt
      601 ccctgccggc gcccccgggg cgtacgcatc tttcggcaag actgcgattg gaaatccgcg
      661 ttggcgcttt aacgtctgcg gcaagacgtt ctcgcagaac ctcaaggcta ccgcccggca
      721 gagggagcct cataagaata agacgatatt caaactgttg gtcaacaaga tgccggtccg
      781 ccgcatcatc gaagtcacgg atatcgatcc acagaccttc tataatcgcc tcggtttcct
      841 ccatcggcag tgccaattat ttgctgctcg ccgcgagagc gccctcgccg atcttccgat
      901 ccgccgactg tacctgggcg tcgatcgcca ggattacctg gtgaattggt tgggccgcaa
      961 ggacaaacgc aacacccggc tatcggctat tgccgtggtg gacaacgaat atggctattg
     1021 ctttggagcc cacctcaact tcgacccttc gttggataga aaagcgattc aaaccgaagt
     1081 tgagaaaagc ggcgacttga gcaagcctta tccgcatcgc cgctttgctc gtctctggat
     1141 tgatgcagac cacgagaagg ccagcgcgaa gtccatgatc gcgaagcagc gcaaattggg
     1201 cttggcgatg gagatcgatg aaacctacag cgcagccctg acgcgggacg acatcgaaag
     1261 ccccgatacg ccttcacccg cgacgcggct tcccgaatac ggcatgcaga tccatggcga
     1321 gtacacgatg tacggctgtt ttttcttcct caaacgcttg ttgggcaatg tccagaagtg
     1381 gcggtttttt cttgaccagg acccaggcct tagggcggcg tgtctagcgg cattccgcaa
     1441 tgaaattcaa agccggacag cggacgcctt ttatgtcggc gtcgccaagg acctgacggt
     1501 ggatgagaag cggcaaaaat acctcgaagc gaaggcgcgg ttcgagaagg cgcagaccgc
     1561 gcacccggcc atgaagccca aggaggtcaa gctaatgctg atcaaagagc gtctggccac
     1621 catgacgcct cacgggaaat gggaagaccg ttggctcgat catcctttcc caacgatggg
     1681 cgaaccggag aaggccgtgg cctacctcac cgatttcggc gattacgacg aggatcaccg
     1741 ggcttggctc tacaacaagg ccagcctgca cggcgtcaat gccttcttca atcaagtgcg
     1801 ccggcggctt tcctttcttg aaaggccgat ccactccaaa acaaacaaag gccggatctg
     1861 gtatggctac tctccgtatg atccaggcaa tgtcataaaa gtcctcgaca tcatgcgcac
     1921 cgtccacaac tacatcctga ccggaaagga tggcaaaacg ccagccgagc gccttgggct
     1981 ggctcaagcg ccactggact acgaagacat tatttactta tcttgatggc ttcgcgcagg
     2041 acagccccct ctgccgcaat cgtcaacaga ttaagtcaac ggcttgagaa catggggtgg
     2101 aaggattccg gcgatgcggt gatgtatccc gatgggactt tagtccgggg tccgaaagct
     2161 ctttgcgaat tgca
//
LOCUS       DATMGX010000314         2738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_98952, whole genome shotgun sequence.
ACCESSION   DATMGX010000314 DATMGX010000000
VERSION     DATMGX010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98952"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VNR65_05110"
     CDS             complement(<1..1461)
                     /locus_tag="VNR65_05110"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HWJ18093.1"
                     /translation="MGQEVGSGADLAEDQPLPATLVRTPLAGVFRRFINLSFDLGSIA
                     LVVASAVLTGGVIAAHVLGRGFEWQDELEIFLVAGAVFLSSAAVQARRGHARIGVFDS
                     LLSESAQRRWTLVTDALSLIFVAFIAWKSGTLCLEAWTEGQVSQSTWAPPMWIPYGIM
                     TLGLALLACELARQVRETSTFALLAALLTLAALLLWQRPVTPVLSGFTPVSIGIAYCT
                     VTLVVMFSGMPIAFALGVVATAFMILFMPRASVDTIAQNVYEELANVILLAIPLFILK
                     GAAIGRSDAGRDLYTALHAWLHRIPGGLGIANTVACGLFAAMAGSSPATCSAIGSAGI
                     PEMRKRGYSPGLASGIIAAGGTLGILLPPSVTMLLYAVAAEVSLGKLFLAGIGPGLLL
                     IVLFAAYAAFRFRRERNAARRAVARGGKPSAILEEHPYTMREKLLMLAWVTPFVTILA
                     GVMIVLYAGLATPSETAGVGAILALLVIGVMYGVWRP"
     gene            complement(1461..2471)
                     /gene="dctP"
                     /locus_tag="VNR65_05115"
     CDS             complement(1461..2471)
                     /gene="dctP"
                     /locus_tag="VNR65_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HWJ18094.1"
                     /translation="MISRRAFVGGAALSLAAPSVLRAQTARTLKISHQFPGGTIDQGD
                     FRDRLCRKFAAAVEERTGGALKFEIYPGSSLMKTNAQFSALRKGALDFSLYPLAYAGG
                     EVPAMNLGLMPCLVTSYEQGAKWKEAPIGKSLARIAEEKGVKFISWIWQAGGIASAKA
                     IKAPDDVKGIKIRGGSREMDLVLSAAGASVSTMPSNEIYLGMQTGALDAAMTSSTSLI
                     SFRLEELAKAVTTARAGSIWFMCEPLLMSKDVFESLPPDQQKAITDAGQEMEWFGAIE
                     AKRDDGAVAKVYAEKGATVVDFSADDLAKWRAISEVSAWKDFASKSAEAAELLASARS
                     VA"
BASE COUNT          575 a          821 c          846 g          496 t
ORIGIN      
        1 cggacgccag acgccataca tgacgccgat cacgagcagc gcaaggatcg ctccgacacc
       61 tgcggtttcc gacggagtcg caagcccggc gtagaggacg atcatcacgc cagccaggat
      121 cgtgacgaag ggcgtcaccc aggcgagcat caggagcttt tcccgcatcg tatagggatg
      181 ctcttcgagg atggcgcttg gtttgccgcc ccgcgccacg gctctccggg ctgcgttgcg
      241 ctcgcggcgg aagcggaaag cagcgtaagc cgcgaacagc acgataagaa gtaaacccgg
      301 cccgataccg gcaagaaaca gcttgcccag cgaaacttcg gcggcaacgg catacagcag
      361 catggtgacg gacggcggca ggagaatgcc gagcgttccc ccagcggcga tgatgcctga
      421 cgcgaggccg ggcgagtagc cgcgcttgcg catctcgggg atgcccgccg aaccgattgc
      481 cgagcaggtg gcgggcgacg atcctgccat cgccgcgaag agaccgcagg ccacagtatt
      541 cgcgatgcca aggccgccgg ggatgcgatg gagccaggcg tgaagcgcgg tatacaggtc
      601 tcgtccggca tcggatcggc cgatcgctgc gcctttcagg atgaaaagcg ggattgcaag
      661 gaggatgacg ttcgccaact cctcgtagac attctgggcg atggtgtcga ccgaggcgcg
      721 gggcatgaac aagatcatga aggcggtcgc caccacgcca agcgcgaacg cgatcggcat
      781 gccggaaaac atgacgacga gggttaccgt gcaataggcg atgccgatcg acacgggcgt
      841 aaagcctgac aaaacagggg ttacgggcct ctgccagagc aggagcgcgg ccagcgtcag
      901 aagcgcggct aaaagcgcga aggtagacgt ctcgcgcact tgccgcgcaa gttcgcaggc
      961 gagaagggca agcccgaggg tcatgatgcc gtatggaatc cacatcggcg gcgcccaggt
     1021 ggactgtgaa acctgtccct cggtccaggc ttcgaggcag agcgttcccg atttccatgc
     1081 gatgaacgca acgaagatca gcgaaagcgc gtcggtcacc agcgtccacc ggcgttgcgc
     1141 gctctcgctc aaaagggagt caaaaactcc gatgcgagcg tgtccgcgac gcgcttgcac
     1201 cgccgcagac gaaaggaaaa cggcgcctgc cacgaggaaa atttccaact cgtcttgcca
     1261 ctcgaagccg cgaccgagga catgagcggc aatcacgccg ccggtcagaa ccgcgctcgc
     1321 aacgacaaga gcgatggagc cgagatcgaa agagagattg ataaagcgtc gaaagacgcc
     1381 cgctagcggc gttctaacga gcgttgcggg taagggttgg tcttcagcca gatcggcgcc
     1441 ggacccaacc tcctggccca tcaggcgacg gacctggcgc tggcaaggag ttcagcggct
     1501 tcggctgact tggaagcgaa atccttccag gcgcttacct ccgagatcgc gcgccatttt
     1561 gcgaggtcgt ctgccgagaa atccacaacc gttgctcctt tctcggcgta gaccttggcc
     1621 accgccccgt catccctctt ggcctcaatg gcaccgaacc attccatttc ttgtccagcg
     1681 tcggtaatgg ctttttgttg gtcgggcgga agcgactcga aaacgtcctt ggacattaga
     1741 agcggttcgc acatgaacca gatcgagccg gcgcgcgcgg tcgtgaccgc cttggcaagt
     1801 tcttccagcc ggaaggaaat caggcttgtc gaggaggtca tcgcggcatc gagcgcgccc
     1861 gtctgcatgc cgaggtagat ttcgttcgaa ggcatggtcg agaccgacgc cccggccgca
     1921 ctgagaacga gatccatctc ccggctcccg ccgcgaattt tgatgccttt cacgtcgtcg
     1981 ggagccttga ttgcctttgc tgatgcaatg ccaccggcct gccatatcca gctgatgaat
     2041 ttgacgccct tctcctccgc gatgcgcgcc aaggatttcc cgatcggcgc ctctttccac
     2101 ttcgcgccct gctcgtagga ggtgacgagg catggcatga ggccgagatt catcgctggg
     2161 acttctcccc cggcgtaggc cagcggatag agcgagaaat cgagcgctcc ctttcgcaag
     2221 gcagagaatt gcgcgttcgt tttcatcaac gaggagccgg ggtaaatctc aaatttcaag
     2281 gccccgccgg tgcgctcttc gactgccgct gcgaacttgc ggcaaagccg gtcccggaag
     2341 tcgccctggt cgatcgtgcc gcccggaaat tgatgcgata tcttcagcgt ccgcgccgtc
     2401 tgcgccctga gcacacttgg cgcagccaaa gacaaggcag ctcctcccac gaaggctcgt
     2461 cgtgaaatca tggcatgtct ccagattcgt ttcggcatcg ttggctgtcc ctctctatag
     2521 cattcgctat agcgaaattg ggactctttc ttgctgtgag agggggccgg tccacgtctt
     2581 gttcgtaagg gtggcagtgc ggggtggatg caaggcatgc cttagtttta ctccgtatcc
     2641 gccatggttt tttcatcatc gaccagcaat actgagggag gactatcaaa agagggagaa
     2701 tcgtcatgcg ctcattaacg gcactatgct ttggcctt
//
LOCUS       DATMGX010000315         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_169322, whole genome shotgun sequence.
ACCESSION   DATMGX010000315 DATMGX010000000
VERSION     DATMGX010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169322"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="VNR65_05120"
     CDS             <1..1575
                     /locus_tag="VNR65_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HWJ18095.1"
                     /translation="RIIASIIEDFNRKPRLNNDRGTALLVAASIYDACHYYRFFENTP
                     FGQYCGIITSYEPNHNAISREPTNSDERYKFDTYTNHVLTKGQTTKAYEDEAKRRFIE
                     EPANLKLLVVVSKLLTGFDAPSCTYIYLDNELHDHNLFQAICRTNRLDGDDKDYGYIV
                     DFKELFGDVQEAIAVYSSDELDIDQGCGGDNNVKLNDWLTEGKKQLDAAREGLHYLCA
                     TVPPPREMEQYLHFFCGDASDPNALGATEPLRVSFYKAVATFVRAYAAIASDLSEAGY
                     SDAEVKVLEKEIQFYSDTRSAIKKHSGEELDIKPYEADMRHLLNTYVQADPATDLGNL
                     NSLSLTELIIETGIHDAIARKLNEKGKLSRNAVAEGIINNVRKTIIRDQLTDPRFYAE
                     MSKLLDDLIKQRRIDTAAYEEFLKKAEELVRRMAQKGAGGYPAKLNGYPGAVVLFNNL
                     AHVPAKTFQCPADEDGKAELALQIDLTVRENAPAGWKGDDTREKQVLNALFPIMSRDR
                     EATTAIFGIIKNQPGY"
     gene            1575..2291
                     /locus_tag="VNR65_05125"
     CDS             1575..2291
                     /locus_tag="VNR65_05125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HWJ18096.1"
                     /translation="MNETIRLGEISILVTRKAIKNVHLSVHPPNGRVTLTAPVATRLD
                     VARAYAISRLGWIREQQSRFRNQAREAPRKFVERESHYLWGRRHLLTIVHRETKPFVS
                     LDHRRIKLTVRPGSDARRRAEVLHEWHKSLLHDFVPLLIEKWECKLKVKVDRYFLQRM
                     KTKWGSCNHKAGHIRLNTELAKKPKDLIEYVVVHEMVHLIEPTHSDRFIAIITEHYPS
                     WREARAELNDLLLAAEVWKE"
     gene            complement(2365..>2690)
                     /locus_tag="VNR65_05130"
     CDS             complement(2365..>2690)
                     /locus_tag="VNR65_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18097.1"
                     /translation="EAGEMTILKTSKPRIGGAIPKRDQADLEVDNLSVDRVHKPRKMR
                     ARNREICGFCAAGWRFNRQQLISSVPDSMGSGHKSFALTLAPEADGHIRVDVRRSGRY
                     PRLVQ"
BASE COUNT          684 a          774 c          704 g          528 t
ORIGIN      
        1 cgcatcatcg ccagcatcat cgaggacttc aaccgcaagc ctcgcctcaa taacgaccgc
       61 ggaacggccc tcctcgtcgc ggccagtatc tacgacgcgt gccattatta ccgtttcttc
      121 gagaacacgc ccttcggcca atattgcgga atcatcacgt cctacgagcc taatcacaac
      181 gctatctctc gcgaaccgac caacagtgac gagcgataca agtttgacac gtacacgaac
      241 catgttctga caaaagggca aaccacaaag gcctatgaag acgaggccaa acgacgtttc
      301 atcgaggaac cggccaatct caaattgttg gtcgtagtca gcaaactcct gaccggcttc
      361 gacgcgccaa gctgcactta catctacctc gacaacgagc tgcacgacca caacctattc
      421 caagccatct gccgcaccaa ccgtctcgac ggcgacgaca aagactacgg ctacatcgtg
      481 gacttcaagg aactgttcgg ggatgtacag gaggcgatag cggtctatag ctctgacgaa
      541 ttggacattg accagggatg cggaggggat aacaacgtca agctcaacga ttggctgacg
      601 gaaggaaaga aacagctcga tgctgctcgt gaaggcctcc actatctttg cgctacagtg
      661 ccgccgcctc gcgagatgga acaatatctc catttcttct gcggggacgc cagcgacccc
      721 aacgccctcg gtgcaactga accgctgcgg gtttcgttct acaaggctgt ggccaccttc
      781 gtccgcgcct atgctgcgat tgcctcggac ctcagcgagg cgggttactc agatgccgag
      841 gtcaaagtcc ttgagaaaga gatacagttc tacagcgaca ctcggtccgc catcaagaaa
      901 cactcgggcg aagagctgga catcaaaccc tacgaggcgg acatgcgcca tctcctcaat
      961 acgtacgtcc aggccgaccc tgccaccgat ttaggcaact tgaactccct gtcactcacc
     1021 gagctaatca tcgaaaccgg catccatgac gctatcgcga ggaagctgaa cgagaaagga
     1081 aagctttcgc ggaatgctgt cgccgaagga attatcaata acgtccgaaa gaccattatc
     1141 cgcgaccagc tcaccgaccc caggttctac gcggagatgt cgaagctgct ggatgacctc
     1201 atcaaacaac gtcgcatcga cacagcggca tacgaggagt ttctgaagaa ggcggaggaa
     1261 ctggtaaggc gcatggcgca gaagggcgct ggcggctatc ctgcgaagct gaatggatac
     1321 ccgggagccg tcgtgctgtt caataacttg gcccacgtgc ccgctaagac attccagtgt
     1381 cctgcggacg aagacggaaa ggcagaactg gccctccaga ttgaccttac cgttcgcgag
     1441 aatgctcccg caggatggaa gggagacgac acccgcgaga agcaggtgct caacgcgctc
     1501 ttccctatta tgtcgaggga ccgggaggcg accacagcga tattcgggat aatcaagaac
     1561 caaccgggct actgatgaac gagaccattc ggcttggaga aatttcgatt ctggtgacgc
     1621 gaaaggccat caagaatgtc cacctttccg tccatccgcc aaacggcagg gtgaccctca
     1681 cggctccggt agcaacgcgt ctcgacgttg cacgtgctta cgcgatttca cggcttggct
     1741 ggattcggga gcagcaatca agattccgga accaagcccg agaagcgcct cggaagttcg
     1801 tcgagaggga aagccactac ctgtggggcc gacggcacct gctcacaatt gttcatcgcg
     1861 aaacgaagcc gtttgtttcg ctcgaccatc ggcgcataaa gctcaccgta cgcccaggca
     1921 gcgatgctcg aaggcgcgcc gaagtattgc acgaatggca taaatcgctc ttgcacgact
     1981 ttgtgccgtt actcatcgag aaatgggaat gtaagctgaa ggtcaaagtt gaccgatatt
     2041 tcctgcaacg aatgaaaact aagtggggga gctgcaacca caaggcgggc catattcgtt
     2101 tgaataccga actcgcgaaa aagccgaagg accttatcga gtacgtagtt gttcacgaga
     2161 tggtgcatct catcgagccg acccacagcg accgcttcat cgctattatc acggaacact
     2221 atccctcttg gcgtgaagct cgtgccgaac tcaacgacct gctgctggct gccgaagtat
     2281 ggaaggaatg aaggataacg cagcggcttg cagtattacg aaatgccgta gagggcgcga
     2341 agtgaaacgc cggtctggag aaggtcactg cactaaacgt gggtatcgtc ctgaccgtct
     2401 cacatcaaca cgtatatggc cgtcagcttc gggtgccaag gtaagcgcga atgacttgtg
     2461 cccggaaccc attgaatctg gaacagaaga aatcaactgt tgacgattga atctccatcc
     2521 tgccgcacaa aatccgcata tttccctgtt ccgagcccgc atctttcgcg gtttatgtac
     2581 ccggtccaca gagaggttat caacctccag gtctgcttga tcccttttcg gaattgcacc
     2641 accaatgcgc ggcttggatg ttttcaggat ggtcatctcg ccggcctcct
//
LOCUS       DATMGX010000316         4516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_42609, whole genome shotgun sequence.
ACCESSION   DATMGX010000316 DATMGX010000000
VERSION     DATMGX010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42609"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2410
                     /locus_tag="VNR65_05135"
     CDS             <1..2410
                     /locus_tag="VNR65_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWJ18098.1"
                     /translation="QLTTTGAMLGTAAYMSPEQVRAKELDTRTDLFSFGSVLYEMATG
                     KMPFEGESTGVICAEILHHQPPSASSLNSAIPPALEDIIGDCLQKDRDRRYQLASEVR
                     ADLKQLKHEMILQGVITPDGEEPGMLSSSAGVSEHPSRTSSRARRRSVAAAQSPPSNP
                     PRHFPWKVALPAGVILLALLAGALYYSSHQGKHLTEKDIIVLSDFTNTTSDSVFDDAL
                     KQGLSIQLEQSPFLDLASEHKVNETLKLMGRPAGDRLTPEIAREVCQRGGSKAMLTGS
                     IAKLGNQYVIGLRAVNCATGDTLAEAQEQAAGKESVLKALDAAAVSLRSKLGESLGSV
                     QKYSTPLAEATTPSLEALQAYSLGRRTNSAKGDTAALPFYKRAAELDPTFALAYASMA
                     VAYSNLNEVGRAAETAKKAYDLREKVSERERLSIEANYYLTATGELDKAAQVYELWQQ
                     TYPRDAVPYANLGFISGNLGNWEPASEEDREAMRLEPNNEVTYLNLGSDYAALNQLDK
                     AEAVYKEAEERKLEGENLLSARYTLAFLKDDASQMTQLAAAAVGKPGTEDVLLAAQAD
                     TAAWFGKMRNARDLTRRAVDSAQHNDAKEAAASYQVAAALREVESGNREQARTDAEAA
                     VRLASNRDVRAMAALALARAGDIPGAEKLAGELEQALPLDTLVQKYWLPTIGAAVALQ
                     RKDPNRAVELLRVSSPIELSQPMQVTGSLFPVYLRGEAYLALHDGDAAATEYRKFLNH
                     RGLVANFSWGALARVGLARAYAMQGDTARTRATYQDFLTLWKDADPDVPILKQAKTDY
                     AKLQ"
     gene            2785..>4516
                     /locus_tag="VNR65_05140"
     CDS             2785..>4516
                     /locus_tag="VNR65_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWJ18099.1"
                     /translation="MIGQTISHYRIVEKLGGGGMGVVYKAEDLTLRRFVALKFLPDES
                     AHDPHALARFEREAQAASALNHPNICTIYEIGVADGRTFIAMEFLEGKSLQDFIAGRP
                     LETDVLLSLAIEIADGLDAAHARGIVHRDLKPANIFVTDRGHAKVLDFGLAQVVQTAV
                     VPPSSSDALTKPSSLTLPGVPVGTLQYMSPEQAKGKELDARTDIFSFGAVLYEMGTGI
                     MPFRGDSPAAIFDAILNRDPAPALRLNPELPTRLDDIIQKALEKDPGLRYQHASEVRT
                     DLQRLQRGSDSGRGVRSSDIGRGAKSPGTGTNPARRLGVLAVVLLLVAAVAGYFWRSR
                     RHSGAAGVNTASIAVLPFADMSPGKDQEYFSDGLAEELTNALAKVPGLKVAGRSSAFQ
                     FKGKNEDVRTVGQKLNVANVLEGSVRRDGSRVRITAELTKAEDGFQLWSEEYNTEIKD
                     IFAVQDEIARAVTGALQIKLLGANGTPALARSKVTNPDAYQAYLQAAYFLNRGSEKAN
                     YDKALSYAEQAIKLDPNFSDAWALRSMVVASMATEGFMDTGSGLRRAREDAEQAIALD
                     PHSALGYLALA"
BASE COUNT          954 a         1318 c         1392 g          852 t
ORIGIN      
        1 gcaactgacc actacaggag ccatgctggg cacggcggcc tacatgtcac ccgagcaggt
       61 gcgggcgaag gagttggaca cccgcacgga tcttttttcc ttcggctcgg tgctatatga
      121 aatggcaacc ggcaaaatgc cattcgaggg cgagagtacg ggcgtgatct gcgccgaaat
      181 cctgcatcac caaccaccgt cggcgtcttc cctgaattcc gccatcccgc cggctctgga
      241 ggatatcatc ggtgattgtc tgcaaaagga ccgcgatcgc cgttatcagc tcgcttctga
      301 ggttcgtgcc gacttgaagc aactgaagca cgagatgatc ttgcagggcg tcatcacccc
      361 agatggtgag gaacctggga tgttgtcttc gagtgcgggc gtgagcgagc atcctagccg
      421 caccagttca cgtgctcgtc gccgttccgt cgcggcagcc caatcgccac cgtctaatcc
      481 gccgcggcat ttcccctgga aagtcgcgct tcctgcggga gtcattcttt tagcgctgct
      541 tgcgggtgct ctttactata gctcgcacca gggaaagcac ctgactgaga aggacattat
      601 tgttctctcc gatttcacca acactacgag cgacagcgtt ttcgacgacg cgcttaagca
      661 agggctctcg atacaacttg aacaatcgcc gtttttggat ctggcttccg agcacaaagt
      721 aaatgagaca ttgaagctga tgggccgtcc ggcgggcgac cgcctcacgc cggaaattgc
      781 acgcgaggtc tgccagcgcg gcggcagcaa ggccatgctg accggctcga ttgccaaact
      841 gggcaaccag tacgtgatcg gtctgagggc ggtgaactgc gccaccggcg acaccctggc
      901 ggaggcgcag gagcaggcgg caggcaaaga atccgtactt aaggctctgg acgctgcagc
      961 cgtgagtttg cggagcaaac tgggcgagtc actgggttcg gtgcagaagt actccacgcc
     1021 gttggcggaa gcgaccacgc cgtcgctgga ggcgctgcaa gcctacagcc tgggacgcag
     1081 gaccaactct gcgaagggag acacggccgc gctcccgttc tataagcggg cagcggagtt
     1141 ggacccgact ttcgccttgg cctacgccag tatggcggtc gcctacagca acctgaatga
     1201 agtcgggcgg gcggccgaga ctgccaagaa ggcttacgat ctgcgagaga aagtgagcga
     1261 gcgcgagcgg ttgtccatcg aggcaaacta ttatttgaca gcgaccggag agctcgataa
     1321 agcggcacaa gtctacgaac tgtggcaaca gacctatccc agagacgcgg tgccgtacgc
     1381 caacttggga tttatttcag gcaacctggg aaactgggaa ccggcgtcgg aagaggatcg
     1441 cgaggccatg cgcctcgagc cgaacaatga ggttacctat ctcaacctgg gctccgacta
     1501 cgccgccctc aaccagttgg ataaagcgga ggcggtctac aaggaagcgg aggaacgcaa
     1561 gctggaaggc gagaacctgc tttccgcccg ctacacgttg gcgtttctaa aggacgacgc
     1621 gtcgcagatg acgcaattgg cagccgccgc tgtggggaag ccaggcacgg aagacgtgct
     1681 gctggccgcg caagcagaca cggcggcgtg gtttgggaaa atgcgaaatg cccgcgatct
     1741 gacccggcgg gccgtggatt cagctcagca caacgacgcc aaagaggctg ccgcaagcta
     1801 tcaggtggcg gctgctttgc gcgaagtgga atccggcaac cgcgaacagg cacgcaccga
     1861 tgccgaggca gcagtgaggc tggcctcgaa ccgcgatgtg cgtgccatgg cggccctggc
     1921 tttggcccgg gcaggcgata ttccgggagc ggaaaagctg gccggcgagc tcgaacaggc
     1981 attgccgctg gacactctgg tgcagaagta ttggctaccg acgatcgggg cggctgtcgc
     2041 cttgcagcgc aaagacccga accgcgccgt cgaattgttg cgggtctcga gcccgatcga
     2101 gctgagccag cccatgcaag tcactggctc cctcttcccg gtttatctgc ggggggaagc
     2161 ctaccttgcg ctgcacgacg gcgacgctgc ggcaacggag tatcggaagt ttttaaacca
     2221 ccgaggactg gtggccaatt tctcttgggg agcgctggcc cgcgtcggcc tcgcccgcgc
     2281 ttacgccatg cagggagaca cggcgaggac ccgcgctaca taccaggatt tccttacgct
     2341 ctggaaagat gccgatcccg atgttcccat cctgaagcaa gccaagacgg actatgcgaa
     2401 gctgcagtag acgcccgtgg cgggaagcag tctttcctcg cggctcaatg ttcgtcgatt
     2461 attggcgaaa atgcttagtt ccatgaacgc ttgccgacga ttctgggcga gtgacgggcc
     2521 aaccggaagt tggcctgttc ggagtcaatt tgacgtaaaa cgccaaccct ttcttatgac
     2581 ctacggcgtg tcgtctgctc ccagtcggcg gtttgccgac tatacgtcca agtggatcgt
     2641 ctcccgatgg cgtacccagc gaccaaatcg caggcgctcg gacagtgagt gctatggcga
     2701 tttatcgcca gttatcgact tgaagcccgc caacatcccc ggcttcattg acttgcccgc
     2761 caacccaacg taagattcgc gccaatgatc ggccaaacga tctcgcatta ccgcattgtc
     2821 gagaagttgg gcggtggcgg gatgggggtt gtctacaagg ccgaagacct gaccctccgt
     2881 cgctttgtcg cgctgaagtt tctgccggac gagtcggcgc atgatccgca cgctctggcg
     2941 cgctttgagc gggaagcgca agctgcttcc gcactgaatc accccaacat ctgcactatt
     3001 tatgagattg gcgtggcgga tggccgaacc ttcatagcca tggagtttct tgaaggtaag
     3061 agtcttcagg acttcattgc cgggcgaccc ctcgagacgg acgtgctctt gtcgctggcg
     3121 atcgaaatcg ccgacggcct tgacgctgcc catgcgagag gcatcgttca tcgcgatctt
     3181 aagccggcga acattttcgt taccgaccgc ggacacgcaa aggtactgga cttcggcctg
     3241 gcgcaagtgg tgcaaacggc ggtcgttccg cccagttcca gtgacgccct aacgaaaccg
     3301 tcttcgttga ctctcccggg cgttccggtg ggaacgctgc agtacatgtc gccggagcaa
     3361 gccaagggca aggaactgga tgcgcgcacc gacattttct ccttcggggc ggtgctctac
     3421 gagatgggga ccgggatcat gccgttccgg ggcgacagtc cagccgccat cttcgacgct
     3481 atcctcaatc gcgatccggc gcctgctctg cgtttgaatc cggagctgcc taccaggctg
     3541 gacgacatca ttcagaaggc cctggaaaaa gatcccgggc tgcgctacca acatgcctcc
     3601 gaagtaagga cagacttaca aaggttgcag cgcggatcgg acagcggccg cggagtacga
     3661 agcagtgaca tcggtcgcgg tgcgaagtcg ccagggacgg gaacgaaccc tgctcgcagg
     3721 ctgggcgttc tcgctgtcgt tctgctgtta gtggcggcag tggcaggcta cttctggcga
     3781 agccggcgtc actccggcgc cgccggcgtg aatacggcat ccatcgcagt tctgcccttt
     3841 gccgacatga gcccgggaaa agatcaggaa tatttctcgg atggactcgc tgaggaactg
     3901 acgaacgccc tcgccaaggt acccggcctg aaggttgcag gaagatcctc cgcctttcag
     3961 ttcaaaggca agaacgaaga tgtacgtacg gtaggacaga agctgaatgt agccaacgtt
     4021 ctcgaaggca gcgtgcggcg ggatggcagt cgagttcgca tcaccgcgga gctgaccaag
     4081 gcggaggatg gctttcaact ttggtcggaa gaatacaaca ccgagatcaa agacatcttc
     4141 gcggtgcagg atgaaattgc gcgtgctgtt accggggcac tgcaaatcaa gctgctcggc
     4201 gccaacggaa cacccgccct tgcgaggtcg aaggtcacca accccgacgc ctaccaggct
     4261 tatttgcaag ctgcgtattt cttgaatcga ggatcggaaa aggccaacta cgacaaggcc
     4321 ctgtcttacg ccgagcaagc catcaagctg gacccgaatt tctcggacgc ctgggcgctg
     4381 cggtccatgg tcgtggcaag catggccaca gaggggttca tggacaccgg gtccggactt
     4441 cgccgggctc gcgaagatgc agaacaggcg atcgctctgg acccgcattc ggccttggga
     4501 taccttgcgt tggcgc
//
LOCUS       DATMGX010000317         2471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_258589, whole genome shotgun sequence.
ACCESSION   DATMGX010000317 DATMGX010000000
VERSION     DATMGX010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258589"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VNR65_05145"
     CDS             complement(<1..1051)
                     /locus_tag="VNR65_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HWJ18100.1"
                     /translation="MEPFRHHVFVCTQEKAEGVPCCSAAGSFQILNALHRELGSQGLA
                     DDVQVSSCGCLGICDSGPVMIVYPEGAWYAKLTPNDVPEIVSSHLRAGEKVTRLERIP
                     DAEMKGEILDHRNKYLAMLKGKDAAGVLPDDLNDMIRGFMPSRAILTALELDVFTAVG
                     KGGAAQQIAAKIQAAVRAIEMLLNVLVSLKLLEKHDGVYTNTPVAARFLAEGSPDSAR
                     AAQLHTANLWRRWSNLTDAVRTGTAVESGHDNGWVNSFIAAMDHGARGRARAVAQAVE
                     LNGEKRMLDLGGGSGAYSIAFVKAATALHSEIVDMPEVLPITQEHIRQAGLGDRISTR
                     AGDMLSVPLEPAGYDL"
     gene            1642..2112
                     /locus_tag="VNR65_05150"
     CDS             1642..2112
                     /locus_tag="VNR65_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWJ18101.1"
                     /translation="MQQTFTSKEVVALTGISPRQLQWWDERHIVVPARQGHKRIYALD
                     DLAEVAVICDLRQRGFSLQKIRRVIRFLQKELGKRLVETVTAASEYHLLTDGRHIYLE
                     DSARGVVDLLKNARQPMLSVCLSDTIQRVRADVRGARKRNGSVRHLIARSRRAS"
     gene            2133..2396
                     /locus_tag="VNR65_05155"
     CDS             2133..2396
                     /locus_tag="VNR65_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18102.1"
                     /translation="MIGELSVLSEWIPEQMEPGTIFVLENAGDVGEKEDPYWAVLSCP
                     KCGQLGLVTRKQIAGLLTVICGSEKCSAQFFIRDEEIVQRKPS"
BASE COUNT          492 a          711 c          751 g          517 t
ORIGIN      
        1 ccaggtcgta acctgccggt tcaagtggaa cgctcagcat atcgccggcg cgagtgctga
       61 tgcgatcgcc tagtccggcc tgacgaatat gctcctgggt aatcggcaac acttcgggca
      121 tgtcgacgat ctcggaatgc aacgcggtgg cggctttgac gaacgcaatg gagtacgcgc
      181 ccgagcctcc gccgagatcg agcatgcgct tctcgccatt taattcgacg gcttgcgcca
      241 cggcgcgtgc ccgtccgcga gccccgtggt ccatcgcggc gataaacgaa ttgacccagc
      301 cgttgtcgtg acccgactca acggcagtgc cggtgcgtac ggcgtcggtt aggtttgacc
      361 agcgccgcca gaggttcgct gtatgcaact gcgccgcgcg agcgctgtcg ggagatccct
      421 ccgccaggaa gcgcgccgcg accggtgtat tcgtatagac tccgtcgtgt ttttccagca
      481 gcttaagact taccaggacg ttcagcagca tttcgatggc gcgaaccgca gcctgaatct
      541 tggccgcaat ctgctgcgcg gcgcctccct tgccgacggc agtgaatacg tcgagctcca
      601 gagccgtcag aatggcgcga ctcggcataa agccgcggat catgtcgttg aggtcatccg
      661 gcagcacgcc ggcggcgtcc ttacccttca gcattgcgag atatttgttg cgatggtcaa
      721 ggatctcgcc cttcatctcc gcgtcgggga ttcgctccag gcgcgtgacc ttttctccgg
      781 ccctgaggtg cgacgacacg atctcaggca cgtcgttcgg tgtcagcttc gcgtaccagg
      841 caccctcggg ataaacgatc atcaccggtc cgctgtcaca aatccccaga cagccgcagc
      901 ttgacacctg tacatcgtcg gccagtcctt ggctgccaag ctcgcgatgc agggcgttaa
      961 gaatctggaa cgaacctgcc gccgagcagc agggaactcc ttcagctttt tcctgtgtgc
     1021 aaacgaaaac gtgatgacga aacggctcca cggagacctc caagtcagtt atcagtagcc
     1081 agtagtcagt agccagtggt cagctatcgg actagcacac ttcatcttca agccagcgca
     1141 acgggcgcga aagggcgagg ctcacagcca ggagtgacga cgagcgggac gcagccgaag
     1201 cccgtggccc aaaagaatta aagcggggtc agaattgctg tagtccggcg agtacgggta
     1261 atgcggcgct tcacgtggat tattcatgcg gacttcacgc tttgaagggg cgcggcttca
     1321 gaggctgctg aaaaagccgg tttcagccgg tgttttgaag gggcgcgcct ttaggtgcgc
     1381 cgttaagctc tttgttcttg tcatcacgag cgggcgaacc ggggtcccca acgagcgccg
     1441 cttttacgct tgttggggga aagttcagcc cgcgagggat ctgcttttcc gactttttca
     1501 gcagccctgt tcagccggga aaaattcctc ctgggtgcat ctcccggtta cggttttgac
     1561 caataattca tcttgacact gcaccaaagt gcactatgat gtttcaaaca tttgttgtgg
     1621 gatttgtttt ggggttttgc gatgcagcag acgttcacat ccaaagaagt ggtggccttg
     1681 acgggcatca gcccgcgcca gcttcagtgg tgggatgagc gtcacatcgt ggttccggct
     1741 cgtcaagggc acaagcgcat ctatgcgctg gacgatctgg ccgaggtggc cgtcatttgc
     1801 gatctgcgcc agcgcggctt ctcgctgcag aagattcgcc gggtcatccg cttcctgcaa
     1861 aaggagcttg gcaagcgcct ggtggagacg gtcaccgccg cctccgagta ccacctgctg
     1921 accgatggcc ggcatattta cctggaagat tcagcccgtg gggtggtcga tctgctgaag
     1981 aacgcccgtc agccgatgtt atcggtgtgc ctgagcgata ccatccagcg ggtccgcgcc
     2041 gacgtgcgcg gagcgagaaa gcgcaacggt tccgttcgtc atcttattgc acgaagtcgt
     2101 agggcttctt gaacggtcct gaaggagggc acatgatcgg agaactgagc gtcctgagcg
     2161 aatggatccc cgaacagatg gagcctggaa ccatctttgt gttggaaaat gctggcgacg
     2221 tgggcgaaaa ggaagacccc tattgggctg ttctgtcgtg cccgaaatgc gggcagctcg
     2281 ggctggtcac ccgcaagcag atcgcgggct tgctgacggt catttgcggt tccgagaagt
     2341 gctccgccca gttcttcatc cgggatgaag agatcgtgca gcgcaagccg agctaaccgg
     2401 ggcagctatc ggccatcagc taagacgcac tgcaaccgga aggcagaccg ttatgcttga
     2461 cgtcacgcat g
//
LOCUS       DATMGX010000318         2517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_204963, whole genome shotgun sequence.
ACCESSION   DATMGX010000318 DATMGX010000000
VERSION     DATMGX010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204963"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1809
                     /locus_tag="VNR65_05160"
     CDS             <1..1809
                     /locus_tag="VNR65_05160"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HWJ18103.1"
                     /translation="DELFEVIEARRVEVMRSIIARHGAALGKYQGDFESALKGLDKQK
                     AVSRMWKKDPTVWKDGPEHEKIIGNALGWLKVAETMQPHVAELLAFAEEIRKAEFEYA
                     VVLGMGGSSLCPEVLGRTFGKKDGYPQLYVLDSTVPTAIRHLEQKIDVGKTLFIVSSK
                     SGDTTEPQIFQRYFHDRVKRVKGDMAGENFVAVTDPGTQLTKDAAHYRFRRVFLNMPD
                     IGGRYSALSYFGMVPFAVMGGDVKSLLEHTLQAQHACVGPVGINENPGARLGAMLGSL
                     AQAGRNKLTFVTPAPLDSLGLWIEQLIAESTGKEGKGIVPVAGEPLGSPNDYGDDRIF
                     AFIHTQSSKTGDIDAKLAALEAAGHPVLRHALHDPLDLGEEFFLWEFATAIAGALLGI
                     DAFDQPNVQESKDNTKRLLGEYVQNGSLAQQKLIIAEESMRVFGDEATRDLLRRGGSS
                     MQEILTAHLSRIKPGDYFAVTPYIEELNNYEGLLQQVRIAVRDEKKVATTSGYAPRFL
                     HSTGQLHKGGPDTGVFLQITSEDITDIEIPAEKFTFGVLKQAQVLGDFESLSARSRRA
                     IRVDLGRDIEKGLRRLLVLIKEAVSQATAAEAGVRH"
     gene            1820..2503
                     /locus_tag="VNR65_05165"
     CDS             1820..2503
                     /locus_tag="VNR65_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWJ18104.1"
                     /translation="MPTTSPDLKAAQFGIIGLGVMGENLALNLDDHHQSVAVWNLETE
                     WVDRFVQNNADRHFVGAKSLQQFCHSLERPRRILMMIKAGDPVDQTLQKITPYLTPGD
                     IVLDGGNSYFKDTIRREASTRHILVHYFGMGVSGGKEGARYGPSLMPGGARAAYELVA
                     PILQAIAAKTDCGPCVTYVGPDGAGHFVKMVHNGIEYGDMQLIAEAYEILNRALGLDA
                     QELSDIFGE"
BASE COUNT          515 a          772 c          753 g          477 t
ORIGIN      
        1 gatgagctgt tcgaagtgat agaagcccgt cgtgtcgagg tgatgcgctc catcattgcg
       61 cggcacggcg ccgcgctcgg caaatatcag ggcgatttcg aaagcgccct caagggcctc
      121 gacaagcaga aggctgtctc ccgcatgtgg aagaaggacc ccacagtgtg gaaggacggt
      181 cccgagcacg agaagatcat cggcaacgcc cttggctggc tcaaggttgc cgagactatg
      241 cagccgcatg ttgccgagct gctcgcgttc gcagaggaaa ttcgcaaggc tgaattcgaa
      301 tatgccgtgg tgctgggtat gggcggatcg agtctgtgtc cggaggtgct cggccgcacg
      361 ttcggcaaga aagacggcta cccgcagctc tacgtcctcg actccaccgt gcccacggcc
      421 atacgccacc tggagcagaa gatcgatgtc ggtaagacac tcttcatcgt gtcgtcgaag
      481 tcgggcgaca caaccgaacc tcagatcttt cagcgctatt ttcacgatcg ggtgaagcgg
      541 gtcaagggcg acatggcggg tgaaaacttt gtcgccgtca ccgatcctgg aacgcaactc
      601 accaaggacg ccgcgcacta tcggttccgc cgcgtcttcc tcaacatgcc cgacatcggg
      661 gggcgctact ccgcactatc gtatttcggc atggttccgt tcgccgtgat gggcggagac
      721 gtgaagtcgt tgctcgaaca caccctgcaa gctcagcatg cttgcgtggg accggttggc
      781 atcaacgaaa atccgggcgc ccggctgggt gcgatgcttg gctcgcttgc ccaagcggga
      841 cgtaacaagc tgaccttcgt cacgcctgcg cctctcgact cgctgggcct ctggatcgaa
      901 cagctcattg ccgagagcac cggcaaagaa ggaaagggca ttgtgccggt tgccggcgag
      961 ccgctgggtt cgccaaacga ttacggcgac gatcgcatct tcgcgttcat tcacacgcag
     1021 tcgtccaaga ccggcgatat cgatgccaag ctggcagcgc tcgaggctgc cggccatccc
     1081 gtgctgcgcc atgcgctgca cgatcctctc gatctgggcg aggagttctt cctgtgggaa
     1141 tttgccaccg cgattgcggg agcgctgctc ggcatcgatg cattcgatca gcccaacgtg
     1201 caggaatcga aggacaacac caagcggctg ctcggcgagt atgtgcagaa cggttcactt
     1261 gcacagcaga agctgatcat tgccgaggaa tcgatgcgcg tctttggtga cgaagccact
     1321 cgagatctac tacggcgcgg tggctcgtcc atgcaggaga tcctcaccgc gcacctttcg
     1381 cggatcaagc cgggcgacta cttcgctgtc accccataca tcgaagagct gaacaactat
     1441 gagggcttgc tgcagcaggt ccgcatcgcc gtgcgtgacg agaagaaagt ggccaccacc
     1501 agcggctacg cacctcgctt cctccattcc accggacagt tacacaaggg cggtcccgat
     1561 accggcgtgt ttctccagat cacctccgag gacatcacgg acatcgagat ccccgccgag
     1621 aaattcacct tcggcgtgct caagcaagcg caagtgctag gcgatttcga gtcactgtcg
     1681 gctcgcagcc gccgcgccat tcgcgttgac ctcggacgcg acattgagaa gggactccgc
     1741 cgcctgcttg tgttgataaa ggaagcggtc tcgcaggcca ccgccgcgga agccggcgtt
     1801 cgccactaag gagcgactca tgccaaccac gagtccggac ctgaaggcgg cgcagttcgg
     1861 cattatcggc cttggcgtca tgggcgagaa cctggcgctc aaccttgatg accaccatca
     1921 aagcgtcgcg gtatggaacc tggagacgga gtgggtagac cgcttcgtac agaacaacgc
     1981 tgaccgtcac ttcgtgggag ccaagtcgct gcagcaattc tgccactccc tggaacggcc
     2041 gcgccggatc ttgatgatga tcaaggctgg ggacccggtg gaccagacgc ttcagaagat
     2101 cactccgtac ctgacgcctg gcgacattgt cctggatggc ggcaactcct acttcaagga
     2161 caccattcgt cgcgaggcca gcacccgcca tattctggtc cattacttcg gtatgggcgt
     2221 ttccggcggc aaggaaggcg cgcgctacgg tccgtccctc atgccgggcg gcgcgcgtgc
     2281 cgcttatgag ctcgttgccc cgattctgca agccatcgct gccaaaaccg actgcggccc
     2341 ctgcgtcacc tacgttggcc ccgacggcgc tggccacttt gtaaagatgg ttcacaatgg
     2401 catcgagtac ggcgacatgc agctcatcgc cgaggcttac gagatcctga atagagcgct
     2461 cggtttagat gcgcaagaac tgtcggatat cttcggggag tgaaaccgca gcccgct
//
LOCUS       DATMGX010000319         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_295092, whole genome shotgun sequence.
ACCESSION   DATMGX010000319 DATMGX010000000
VERSION     DATMGX010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295092"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VNR65_05170"
     CDS             complement(<1..461)
                     /locus_tag="VNR65_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWJ18105.1"
                     /translation="MPETGERELTFAQAIREALAEEMRRDKTVCILGEDVAEAGTPFK
                     VLAGLLEEFGKDRVLDTPISEAGFTGLAVGAAMTGLRPVVDIMFGDFITLTMDQMVNQ
                     AAKVHYMSGGKWRVPMVMRTTLGASRRSAAQHSQSLHAWFCHVPGLKVILPS"
     gene            complement(454..1446)
                     /locus_tag="VNR65_05175"
     CDS             complement(454..1446)
                     /locus_tag="VNR65_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWJ18106.1"
                     /translation="MTIAAHPKEASAQIELWLRMYRQMVAIRQFEAQVNELYTRALMP
                     GLAHLYIGEEAVAVGVCEALDRTDYITSTHRGHGHCLAKGASPDQMFAELLGKKAGYC
                     KGKGGSMHIADPATGNLGANAIVCGSAGIATGAAFSAQRLGNGRVAICFFGEGALGQG
                     VLYEVMNLAQLYKLPVIYVCENNLYTEYTHYSETTAGDILMRATAFGLEAASVNGQDV
                     RAVYGIAQRLVDRARRGDGPAFLLCNTYRYHGHHVGDINREYYRSKQEELTWKTERDP
                     IANFGKWLVEQKLADVAVLDRVQAELESEMKSAVDFAVAAPYPAVGEVDEDVYA"
     gene            complement(1443..>2408)
                     /locus_tag="VNR65_05180"
     CDS             complement(1443..>2408)
                     /locus_tag="VNR65_05180"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ18107.1"
                     /translation="GFVFIGKEKFSYQTEFISADLQAKGNREMAKLGFIGLGVMGGNM
                     VARLLEKGHTVTGYNRTRSKAQWLIDKGMKFAESPKAVAAASDVPFTMVTNAAALAAV
                     TDGPGGLLAGIGAGKFHIDMSTVSPDVSRALAASVREKGADMIDAPVSGSVITLQQGK
                     LSVMVGGRKETFEHLRSVLFDIGPKVTHVGDNGVALSMKIAVNLQLAVQMLAFCEGVL
                     LAEKSGIAREVAVDVMIHSAIASPMIVYRGPFVLQQPEEAWFDCNMMQKDMLLAMDLG
                     RKLNVPVPTTAISNEFLTAARGMGWEKLDFAVMFDVLARMSGVPK"
BASE COUNT          475 a          771 c          654 g          508 t
ORIGIN      
        1 gaaggcagaa tcaccttcaa acctggtaca tggcagaacc acgcgtgcag cgactgcgag
       61 tgttgcgccg cggagcggcg actcgctccc aacgtggtgc gcatcaccat cggcactctc
      121 cactttccac ccgacatgta atggaccttg gcagcctggt tgaccatctg atccatggtc
      181 aaagtgatga aatcgccgaa catgatgtcg accaccggac gcagtccggt catggctgcg
      241 cccacggcaa gcccggtgaa gcccgcttcg gagatcggcg tatccagcac gcggtctttg
      301 ccgaattcct ccaaaaggcc agctagaacc ttgaacggcg tgccggcctc ggccacgtcc
      361 tcgcccagaa tacagacggt cttatcgcgg cgcatctctt ccgccagggc ctcgcgaatc
      421 gcctgcgcga aggttagttc acgctcgccg gtttcaggca tacacgtcct cgtccacttc
      481 accgacggcc gggtagggcg ccgccacggc gaagtcaact gcactcttca tctcggactc
      541 gagttcagcc tgcacccgat caagcaccgc aacatcggcg agtttctgct cgaccagcca
      601 ctttccgaag ttcgcgatcg ggtcccgctc cgttttccat gtcagttctt cctgcttgga
      661 gcgatagtat tcgcggttga tgtcgcccac gtgatggccg tggtaacgat acgtgttgca
      721 gagcagaaac gccggaccat caccgcgccg ggctcgatcc accaagcgct gggcaatgcc
      781 gtaaaccgca cgtacgtcct gaccgttcac gctggcggct tccaagccga aggctgtcgc
      841 ccgcatcagg atgtctccag cggtggtttc cgagtagtgg gtatattcgg tatagagatt
      901 gttctcgcag acgtaaatca cggggagctt gtaaagctgg gcgaggttca tcacttcata
      961 cagcacaccc tggccgagcg cgccttcccc gaagaaacat attgcgaccc ggccgttacc
     1021 gagacgctga gcggaaaacg cggctccggt ggcgataccg gcgctgccgc atacgatcgc
     1081 attcgctccc aggttgccgg tcgccgggtc cgcaatgtgc atggagcctc ccttgccctt
     1141 gcagtacccg gccttcttgc ccagcagttc ggcgaacatt tgatcggggg aggcgccttt
     1201 cgccaaacaa tgtccatgac cgcgatgtgt actggtgatg taatcggtgc gatcgagcgc
     1261 ttcacataca cccaccgcga ccgcttcctc accgatataa agatgcgcca gaccgggcat
     1321 taacgcccgc gtgtaaagct cgttgacctg cgcttcaaac tggcggatgg cgaccatctg
     1381 ccggtacatt cggagccaca actcgatttg cgcagaagct tctttcgggt gtgcggcgat
     1441 cgtcatttgg gcacccctga catgcgcgcc aatacgtcga acatgaccgc gaagtccagc
     1501 ttctcccagc ccattccgcg cgcagcggtc agaaactcgt tgctgatggc ggtggtgggg
     1561 acggggacat tcagcttgcg gcccaggtcc attgccagaa gcatgtcctt ctgcatcatg
     1621 ttgcagtcga accacgcttc ttccggttgc tgcaggacga agggcccgcg atacacgatc
     1681 atcggcgatg cgatcgcgct gtgaatcatt acgtctacgg caacctcgcg ggcaattccg
     1741 ctcttctccg ccagcagtac tccctcgcag aacgccagca tctgtactgc taattgcagg
     1801 ttcaccgcga tcttcatcga taacgctacg ccattgtcac ctacgtgcgt tactttcgga
     1861 ccgatatcaa acagcacaga cctcaggtgc tcaaacgttt ccttacggcc tcccaccatt
     1921 accgaaagtt tgccttgctg cagagtgatg acgctgccgg agaccggagc atcgatcatg
     1981 tcggcaccct tctcgcgaac gcttgcagcc agcgcccggc tgacatcggg gctcacggtg
     2041 ctcatatcaa tgtggaattt tccagcaccg atgccggcga gcaaaccacc cgggccgtcc
     2101 gtcaccgccg cgagcgcggc ggcgtttgtt accatggtga agggcacatc cgaggcagcc
     2161 gccacggcct tcggagactc ggcaaacttc atgcctttgt cgatcaacca ctgcgctttg
     2221 gagcgcgtcc ggttgtatcc ggtcaccgtg tgcccctttt cgagaaggcg cgccaccatg
     2281 ttgccgccca tcacaccgag accgatgaat cctagtttgg ccatttcacg attccctttc
     2341 gcttgtaaat ccgcgcttat gaattcggtt tggtatgaga acttttcttt gccgatgaag
     2401 acgaatcc
//
LOCUS       DATMGX010000320         2396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_262216, whole genome shotgun sequence.
ACCESSION   DATMGX010000320 DATMGX010000000
VERSION     DATMGX010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262216"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VNR65_05185"
     CDS             complement(<1..1333)
                     /locus_tag="VNR65_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ18108.1"
                     /translation="MASVAATAQTSASQSESATPDAKPAQSAPRKVDKAAAYYHYTMA
                     HMYEEMVTAYGRSDLATKATEEYRLAIEADPSSEFLTSALAELYVKTGRIADAVREAQ
                     DIIKRDPKNLEAHKLLGRIYLRSLGDMPGGSGSDNILKLAIDQYEQITKLDPDSVDDH
                     LLLGRLYRLNNDLQKAEGELKTAIKLDPSSEEAVTTLAMLYTDEGDTAHALKVLSSIP
                     DSARSAKLYAALGAAYEQRKDYKSAIDAYKHAIVLDRDNLDAIRGLAENLLNDGQLDA
                     SLEQYKVIADSNPEDAQTYVRIAEIYRRQAKYDLALENLKRADTLVPDTMDVPYSMAS
                     VYQAQGRYDDAIKLLQDLVKRTEKSEAGSSNADRNNRAIFIERLGMVYREQENYTAAV
                     ETFRKMLPLGDENARSGYQEMIDTYREAKQWPQATAVAKEAVQKMPGDRDLR"
     gene            1332..1547
                     /locus_tag="VNR65_05190"
     CDS             1332..1547
                     /locus_tag="VNR65_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18109.1"
                     /translation="MIENIKKERKTLFMLDLMRWKRVSETSASLDAKPPGERCLYSTA
                     TGGGMGKNTASRVNAEGTGGTGTVGNC"
     gene            complement(1633..>2396)
                     /gene="purH"
                     /locus_tag="VNR65_05195"
     CDS             complement(1633..>2396)
                     /gene="purH"
                     /locus_tag="VNR65_05195"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF013931.4"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWJ18110.1"
                     /translation="AAWDLAQEFEEPVVAIIKHTNPCGTATGKTLAEAYKRAFECDPV
                     SAFGGVIGVNRPIDAYAAEEMHKLFLEVIAAPAFDEAAKVKFASKKNLRLVEVKAVNQ
                     KWVLKNVSGGMLVQDSDVRPLQDADLKVVTKRAPTPEETRALLFAWKVCKHVKSNAIV
                     YARDGQTVGVGAGQMSRVDSAKIGAMKAQLPLKGTVAASDAFFPFPDGVEEIAKAGAT
                     AIIQPGGSQRDPEVIEAADRLGLAMLFTGVRHFRH"
BASE COUNT          431 a          611 c          802 g          552 t
ORIGIN      
        1 tgcggaggtc gcggtcaccg ggcattttct gaacggcttc tttggcgacg gcggtggctt
       61 gcggccactg tttggcctcg cggtaggtgt cgatcatttc ctggtagccg ctgcgggcat
      121 tctcgtcgcc caggggcaac atcttgcgga aggtctcgac ggcggcggtg taattttcct
      181 gctcgcgata gaccatgccg aggcgctcga tgaagatggc gcggttgttg cggtcggcat
      241 tgctggatcc ggcttcactc ttttccgtgc gcttcaccag atcttgcagg agtttgatcg
      301 cgtcgtcgta acggccctgc gcctggtaaa ccgaggccat gctgtaggga acatccatgg
      361 tgtcgggcac cagcgtgtcc gcgcgcttca gattttcgag agcaaggtcg tacttggcct
      421 ggcggcgata aatctcggcg atccggacat aggtttgcgc gtcttcggga ttggagtcag
      481 caatgacttt gtactgttcg agggacgcgt cgagttgccc gtcgttcaac agattctcgg
      541 ccagaccgcg aatggcgtcg aggttgtcgc gatcgagaac aattgcatgc ttgtaagcat
      601 cgatggcgct cttgtagtcc ttgcgctgtt cgtaggcagc gccgagagcg gcgtacagct
      661 tggccgaacg ggcggagtcg ggaatggaac tcagtacctt gagcgcgtgg gccgtgtcgc
      721 cttcatcggt gtagagcatg gccaatgtgg tcacggcttc ttccgagctg ggatcaagtt
      781 tgatggcagt cttgagttcg ccttccgctt tctgaagatc attgttcaag cgatagaggc
      841 ggccgagcag caggtgatcg tcgacgctgt cgggatcgag cttcgtaatc tgctcgtact
      901 ggtcgatggc cagcttcaaa atattgtcgg agccggaacc gccgggcata tctccgaggg
      961 agcgcagata aatgcggccg agaagcttgt gggcttcgag gttcttgggg tcgcgcttga
     1021 tgatgtcttg cgcttcccgg acagcgtcag cgatgcggcc ggtttttacg tagagctcag
     1081 ccagggcgga agtcaggaac tctgacgacg gatcggcttc gatggcgagg cggtattctt
     1141 cggtggcctt ggtggcgagg tcgctgcgac cgtaggccgt gaccatctcc tcatacatgt
     1201 gcgccatcgt gtaatggtag taagcagcgg ctttgtcgac tttgcgcggc gcagactgag
     1261 ccggcttagc gtcgggcgtg gcggattccg actgagacgc gctggtttgg gcggtcgcgg
     1321 ccaccgaggc catgatcgag aacatcaaaa aagaacgaaa aactctgttc atgctggacc
     1381 tgatgcgctg gaagcgcgtt tccgaaacct cagctagttt ggatgcaaag ccgccggggg
     1441 aaaggtgcct atattctacc gcgaccggtg gcgggatggg aaaaaacact gcgagtcgcg
     1501 tgaatgctga aggaaccgga ggtacaggta ccgtggggaa ttgctgattg ttgatttttg
     1561 attgttgttt gaacaccggc ttcaaggatc tggggaacac ctgtctttac aatcaaggaa
     1621 tcaacgattt acctagtggc ggaagtggcg cacgccggtg aagagcatgg ctaggcccag
     1681 gcggtcagcg gcttcgatca cctcgggatc gcgctgcgag cctccgggtt gaatgatggc
     1741 cgtggctccg gctttagcga tttcttccac cccatcggga aacgggaaga aggcgtcgga
     1801 ggcggcgacg gtgcctttga gcgggagttg ggctttcatg gcgccgatct tggcggagtc
     1861 tacgcgggac atttggccgg cgccgacgcc gacggtctgg ccgtcgcgcg cgtagacgat
     1921 ggcgttggat ttcacgtgct tgcagacttt ccaggcgaag agcagggcgc gggtttcttc
     1981 gggcgtggga gcgcgcttgg tgacgacttt gagatcggca tcttgcaggg gacggacgtc
     2041 ggagtcctga acgagcattc ctccggaaac attcttcagc acccacttct gattcaccgc
     2101 ctttacttcc acgaggcgga gatttttttt cgacgcgaac ttgacctttg cggcttcgtc
     2161 gaaggcgggg gcggcgatga cttcgaggaa gagcttgtgc atttcttcgg cagcgtaggc
     2221 atcgataggg cgattcactc cgatcacgcc gccgaaggcc gagactgggt cacattcgaa
     2281 ggcgcgcttg taggcttcag cgagcgtctt gccggtggcg gtgccgcagg gattggtgtg
     2341 tttgatgatg gcaacgacgg gctcctcaaa ttcttgggct aaatcccacg cggctc
//
LOCUS       DATMGX010000321         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_329327, whole genome shotgun sequence.
ACCESSION   DATMGX010000321 DATMGX010000000
VERSION     DATMGX010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329327"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VNR65_05200"
     CDS             complement(<1..737)
                     /locus_tag="VNR65_05200"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HWJ18111.1"
                     /translation="MARRAGSADLPLHNGRVPPWLGQRMARLGAVIAEAIVHHYGRDE
                     FLRRLAHPFWFQSFGAVMGMDWHSSGITTSVLGALKRGLGPISDELGLYVCGGKGSHS
                     RQTPHELAAIGERTGFDGEALAKASRLLAKVDSAAVQDGFDLYLHGFIVTADGQWAVV
                     QQGMNDNTGMARRYHWLSEGLESFVTAPHSAIDGENQGEIINLTDRRAEASRLKQLDL
                     LQNLGPDGIVCEISALEGRGGETNQPFL"
     gene            919..1314
                     /locus_tag="VNR65_05205"
     CDS             919..1314
                     /locus_tag="VNR65_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWJ18112.1"
                     /translation="MEPLSDRLSSHLLSLLRIVAALIFIEHGTQKLFGFPAAPANGYP
                     EFLSLFWWQGVIEVVGGALLLLGLFTRFVAFILSGNMAVPYWMVHAPKSFYPALNGGD
                     AAILYCFVFLFMAAAGGGAWSVDRLLKRS"
     gene            1712..>2270
                     /locus_tag="VNR65_05210"
     CDS             1712..>2270
                     /locus_tag="VNR65_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWJ18113.1"
                     /translation="MPDLTSRQQPAQEGSIVRVACGLIVVSFVLAVLHLGRVVLEPLA
                     LAVLLSFVLSPPIRRLRGLFLGRATSVIAVVVFALAILGALGFVMQMQVSHLAGEIPK
                     YQQNIREKIASVNKALVSSGTLEQASSTLDSLASGLGTKGPNPSPQGQPGNAKGSQQP
                     APIQVEVQKSQSATLQYLQELVSPII"
BASE COUNT          440 a          689 c          652 g          489 t
ORIGIN      
        1 aggaaaggct ggttcgtctc gccaccgcgt ccttccagag ccgaaatctc gcagacgatg
       61 ccatccgggc caaggttttg taagaggtcg agttgcttca gccgggaagc ctcggcgcgg
      121 cggtcggtga gattgatgat ctcgccctgg ttctcgccgt cgatggcaga atgcggggcg
      181 gtgacgaaac tttcgagccc ctccgaaagc cagtgatagc ggcgcgccat cccagtgttg
      241 tcgttcatgc cctgctgaac caccgcccac tgcccatcgg cggtgacgat gaaaccgtga
      301 agatagaggt cgaagccatc ctggaccgcc gcgctgtcga ccttcgcgag gagcctgctg
      361 gccttggcca gcgcctcccc atcgaagcca gtgcgctccc cgatcgccgc caattcgtgt
      421 ggcgtctgac gcgaatggct gccctttccg ccgcaaacgt agaggcccaa ctcgtcggag
      481 atgggtccaa ggccacgttt gagagccccg agaacgctgg tggtgatacc ggaggaatgc
      541 cagtccatcc ccatcaccgc gccgaaggac tggaaccaga aaggatgcgc gagacgccgc
      601 aggaattcgt cgcggccgta atgatgcacg atggcttcgg cgatcacggc tccgaggcgc
      661 gccatgcgct ggccgagcca cggtggcaca cggccattgt ggagaggcag atcggcgctt
      721 ccggcacgtc gggccacgaa tcctccttgg aacgaagtgc gaacgtagca tgctccagcg
      781 ttcaaggcga tgccggcaaa gcgcgcgaat cgcaaaaagc gcagcgttgt ttcactgaat
      841 gtcaggtcag agaaaccttt tggttatctc cgccatttca tcggaattga acactatggc
      901 gtttgcgaaa gaaggatcat ggaaccactt agcgaccggc tgagctcgca cctgctcagt
      961 cttctgagga tcgtcgcggc cctcatcttc atcgaacacg ggacgcaaaa gcttttcggg
     1021 tttccggcgg cgccagcgaa cggttatccg gagttccttt cgcttttctg gtggcagggc
     1081 gtcatcgaag tcgttggcgg cgctctcctc cttctcgggc ttttcacgcg ctttgtcgcc
     1141 ttcatcctct caggcaacat ggccgtccct tactggatgg tccatgcgcc gaagagtttc
     1201 taccctgccc tcaatggcgg cgacgccgca attctctact gcttcgtctt ccttttcatg
     1261 gcagccgccg ggggtggagc ctggagcgtc gaccgccttc tcaagcgctc ttaagcacat
     1321 tctgcaagca agcctgccga gcgttcggct catatcatca attggtgctt ttccaggcac
     1381 gtcatgtgtg gatggctccc ccgttggaag gcaggtcttc agcggaaacc cttttggtac
     1441 atcgatgccg tggaagagag ccatccaccc catttaccaa cggttaaaca cattgggttc
     1501 gtttcgcaaa aaagccataa gtactcatat aagtagttta tccctgcatc aagaacggcg
     1561 atggggaatg gcgtcgggac ttatggccgc ctgtatgatt gcccatttgg aacgctcaag
     1621 ccgcgatcgc gttctccgat caagtccatt ggctcaagcc tgaaatagcc ttgcagccaa
     1681 agcgttagca ttgcacggga agaaggcacg catgccagac ttaacgtcgc gacagcagcc
     1741 ggcgcaggaa ggaagcatcg ttcgtgtggc gtgcggcctt atcgttgtca gtttcgtgct
     1801 tgccgtcttg catctcggcc gcgtcgtgct ggagccgctc gccctagccg ttctgctctc
     1861 tttcgtgctg tcgcctccca tcaggcgctt gcgcggtctt tttcttggaa gagcgacgtc
     1921 ggttattgct gttgtggtgt ttgccctcgc catcctcggg gcgctgggct tcgtcatgca
     1981 gatgcaggtt tctcatctgg ccggcgaaat tccgaaatat cagcagaaca tccgcgagaa
     2041 aatcgcctcc gtgaacaaag ctctggtctc ttcgggcacg ctcgaacagg cgtcctcgac
     2101 attggacagc cttgcctcgg ggctgggcac taaaggcccc aatccttccc cccaaggcca
     2161 gcccggcaac gccaagggaa gtcagcagcc cgctccgata caggtggagg tgcaaaagtc
     2221 gcagtccgcc accctccagt accttcagga attggtcagc cctatcatcg
//
LOCUS       DATMGX010000322         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_383976, whole genome shotgun sequence.
ACCESSION   DATMGX010000322 DATMGX010000000
VERSION     DATMGX010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383976"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2064
                     /locus_tag="VNR65_05215"
     CDS             <1..>2064
                     /locus_tag="VNR65_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWJ18114.1"
                     /translation="LHGRDPRFGEPSYEDWLHCLHPDDRPRVEKVVFDAIEKRSPEYR
                     TEYRVVLPPGEVRWLEASGRVDYAAGGTPVRMSGIILDITGRKRAEEALREKEEWLRL
                     AVKSSGAAAWQWDILKDEQVWSPESYKLHGRDPKLGPPNYKDWLHYLHPDDRASAEKA
                     VFDAVEKRLPEYRTEYRVVLPSGEVRWLDALGRVEYGKDGTPLHMSGINLDITERKRA
                     EEALRESEGSLRLALKGSGAGAWHWNISTNEMLASLESYEIHGRADMFGKHTTYEDWA
                     ECLHPEDRARAEKLVSHALEQRSPEYTNEYRVVLPSGGVRWVNVLGKVDYAADGTPLR
                     ISGITLDITERKRTEEALRKAEEFHRQKNEELETILAAMPAAVIIAKDADCIEMAGNR
                     AAYDLLRLAPHQDLSKSAPPERAPKNYEVFSNGCRLSADEMPIQRAVAGKNAILTEAL
                     ELRFVEGDSKFVLCNALPLFHENGEVRGAVGAFADITNLKRTEVALRQSEERLKFALE
                     AAGAGTWEVSLETRELRASDKTLAFLGIAPGTPVTHDIGLARVHPDDRSRLEDALRHT
                     LETGQPFILEWRVPFRDGSVRWREARGERRSVSGKQVVAGLVLDITDRKSAEEALRES
                     EARLQFALEAANAGTWEAVPETGEFTASDRALAMHGASPDTPMSLEKALKAVHPEDRQ
                     GAVEV"
BASE COUNT          454 a          565 c          663 g          382 t
ORIGIN      
        1 cgttgcacgg acgcgaccct cggtttggtg agcccagcta cgaagattgg cttcattgcc
       61 tgcatcctga cgatcgtccg cgggtcgaga aggtggtgtt cgacgccatt gagaaaagat
      121 cgccggagta caggacggaa tatcgcgtcg tgctgccccc aggggaggtg cggtggcttg
      181 aagcttctgg cagggtggac tatgccgcag gcggtacgcc agtgcgcatg tcgggcatca
      241 ttctcgacat caccgggcgc aaacgggccg aagaggcact gcgcgaaaag gaggaatggc
      301 ttcgtctggc agtgaagagt tcaggcgcgg ccgcctggca atgggatatt ttgaaggatg
      361 aacaagtctg gtcgcccgaa agctataagc tccatgggcg cgatcccaag ctaggcccgc
      421 cgaattacaa ggattggctc cattacctgc atcccgatga tcgcgccagt gccgaaaagg
      481 cggttttcga tgccgtcgag aagaggctgc ctgaataccg gacggaatac cgcgttgtgc
      541 ttccctcagg tgaagtgcgc tggctcgatg cgctgggtag ggtggagtat ggcaaggacg
      601 gcacgccttt gcacatgtcg ggcatcaacc tcgacatcac cgaacgcaag cgggccgagg
      661 aggcactgcg cgagagcgaa ggatcgcttc gtctggcctt gaaaggctcc ggagcgggcg
      721 cttggcattg gaatatttct acgaatgaaa tgctcgcttc cctggaaagc tacgaaattc
      781 acgggcgcgc cgacatgttc ggcaagcaca ccacctacga agattgggcc gaatgcctac
      841 accctgagga tcgcgcaagg gccgagaagc tggtctcgca cgccctcgag cagagatcgc
      901 ctgaatacac gaatgaatat cgcgtcgttc ttccttccgg tggagtccgc tgggtcaatg
      961 tgctgggcaa ggtggactac gccgcggacg gcacgccttt acgaatatcg ggcatcacac
     1021 tcgacatcac cgagcgcaaa cgcaccgagg aagcgctgcg gaaggcggag gaatttcaca
     1081 gacaaaaaaa cgaagagcta gagaccatcc tggccgcgat gccggctgca gtcattatag
     1141 caaaagacgc ggactgcatt gaaatggcgg gaaatcgtgc agcctatgat cttctgcgtc
     1201 ttgctccgca tcaggatctt tcaaagtcag ccccgcccga acgggcgccg aaaaactacg
     1261 aagtgttctc aaacggctgt cggttgtctg ctgacgaaat gccgatacag cgcgcagtag
     1321 ccgggaagaa cgccattctc acggaagcac tggaactacg ttttgtggaa ggagacagca
     1381 agttcgttct ctgcaacgca ctgcccctgt tccatgaaaa cggtgaagtt cgcggagccg
     1441 ttggtgcctt cgcggacatt acgaatctga agcgcacgga agttgcgctg cgccagagtg
     1501 aggagcgctt gaagtttgcg ctggaagccg ccggcgccgg aacctgggag gtttcgctgg
     1561 aaaccaggga actcagggca tcggataaga cgctggcctt tctcggcatc gcccccggta
     1621 cgccagtgac ccatgatatt gggctcgccc gcgtgcatcc tgatgaccgg tcgcgcctcg
     1681 aggacgctct ccgtcacact ctcgagacag ggcaaccttt tatattggaa tggcgtgtgc
     1741 cgtttcgtga tgggtcggtt cgatggcggg aagcgcgcgg ggaacggcga tctgtctcgg
     1801 ggaagcaggt tgttgccggg ctggttctcg atatcacgga ccggaaaagc gctgaggagg
     1861 cgctgcgcga aagcgaggcg cgtcttcaat tcgcgctcga agcggcgaac gccggaacat
     1921 gggaggccgt gcccgaaacc ggagaattca ccgcgtcgga tcgcgcactc gccatgcacg
     1981 gagcatctcc agatacgccc atgtcgctcg aaaaagcctt gaaggccgtg catcccgagg
     2041 ataggcaggg cgccgtggag gtgg
//
LOCUS       DATMGX010000323         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_288873, whole genome shotgun sequence.
ACCESSION   DATMGX010000323 DATMGX010000000
VERSION     DATMGX010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288873"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VNR65_05220"
     CDS             complement(<1..882)
                     /locus_tag="VNR65_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ18115.1"
                     /translation="MSNFRPIDRQTAYLLPPSVEDWLPERHLARFVVEIVDQLDVSAM
                     SGTYRGSGSASYHPRLLVGLLVYGYATGVFSSRKLERATYDSVAFRFVAANEHPDHDT
                     IAAFRRRFLKEIEALFVKILLLAREMGLLKMGTVALDGTKVHANASKHSALSYEHAGK
                     IEAQLKAEVAELMAKAEAADKADLPNGMSIPDELARREERLKKIAEAKAKIEGRAKER
                     YERELAEHRAKLAAREDNEKATGKKPGGKPPEPPAEGPRPTDQINLTDEESRIMPVSG
                     GGFEQCYNAQAVVAEGSL"
     gene            complement(945..2099)
                     /locus_tag="VNR65_05225"
     CDS             complement(945..2099)
                     /locus_tag="VNR65_05225"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HWJ18116.1"
                     /translation="MDGRAGECEALSEAIVFLDYFNDLADPRQQGKVVYPLREVLLLC
                     LLAVLAGAETFADIARFGEKKLSLLRRFLPFRNGTPAHDHLGDIFAVLDAETFQRCFV
                     SWVAAFTGAPVDVIAIDGKTSRRSYQKKGAKEAIHMVSAFATRQRLVLGQTKVAEKSN
                     EIIAIPKLLGMLAIEGAIVTIDAMGCQREIAKTIVDKRADYVLALKGNQGSLREDVEL
                     FAAEQKAQSFKDTKMSRHETVDGDHGRIETRAYTVINDVAWLQERHDWPGLQSVVIVE
                     STREIGSRIERDTRFYITSLALAAHLIGPVIRSHWAVENSLHWVLDMIFRDDECRVRK
                     DHAPANFTTLKHMAHNLIRKAPGKDSLRLRRKVAAWDDDFLVGLLAACRT"
BASE COUNT          450 a          734 c          665 g          490 t
ORIGIN      
        1 gagactgccc tccgccacaa cggcttgggc gttgtaacat tgctcgaagc cgccgccgga
       61 aaccggcatg atgcgagact cctcatccgt cagattgatc tgatccgttg gccgcggccc
      121 ttcggccggc ggctcgggcg gcttgccgcc gggctttttg cccgtggcct tctcattgtc
      181 ctcgcgcgcc gcgagcttgg ctcgatgctc agccaactcc cgttcgtaac gctctttggc
      241 ccggccttcg atcttcgctt tggcctctgc aatcttcttc agccgctcct cgcgccgcgc
      301 caactcgtcc gggatcgaca tcccgttcgg caggtcagct ttatcggcgg cttcggcctt
      361 cgccatcagc tccgcaacct cggccttcaa ctgcgcctcg attttgcccg cgtgctcata
      421 ggacagcgcg ctgtgcttgc tggcgttggc gtgtaccttc gtgccgtcca gcgcgaccgt
      481 gcccatcttg agcaagccca tctcgcgcgc cagcagcaaa atcttaacga acagcgcctc
      541 gatctccttc aaaaagcgcc tgcggaacgc ggcgatggtg tcgtgatcgg gatgctcgtt
      601 ggccgcgacg aagcggaagg ccacggagtc ataagtggcc cgctccagct tgcggctgga
      661 aaacacgccc gtggcgtagc cgtaaaccag aagcccaacc aataggcgag gatgataact
      721 ggctgagccc gagccacgat aggttccgct catcgctgaa acgtccagct gatcgacgat
      781 ctccaccaca aaccgcgcca gatgccgctc cggcagccag tcctcaaccg agggcggaag
      841 caaataggca gtttgacggt cgatcgggcg gaaattgctc atcaggctct cttccaaggc
      901 ggagcgaatc aatggagcga ctttacccaa tgcttcgcgt caggctaagt ccgacaggct
      961 gctaggaggc cgacgaggaa gtcgtcgtcc caagcggcga ctttacgcct taggcgcagg
     1021 gagtctttgc ccggagcctt gcgaatgagg ttgtgagcca tgtgcttgag cgtggtgaag
     1081 ttggcaggcg cgtgatcctt gcggacacgg cattcgtcgt cgcgaaagat catatccagc
     1141 acccaatgca ggctgttttc caccgcccag tgactacgga tgactgggcc gatcaggtgc
     1201 gccgccagcg ccagtgaagt gatgtaaaag cgggtgtccc gctcgattct gctcccaatt
     1261 tcacgcgtgc tctcgacgat gacgacgctt tgcagccccg gccaatcgtg gcgctcttgc
     1321 aaccaggcga catcgttgat cacagtatag gctctggttt cgatgcggcc gtgatcgcca
     1381 tcgacggtct catggcggct catctttgtg tccttgaagc tttgggcctt ctgctctgcg
     1441 gcaaacagct caacatcctc acgcagagag ccttgattgc ctttgagcgc cagaacgtaa
     1501 tcggcccttt tgtccacaat tgtcttggcg atttcgcgct ggcagcccat cgcatcgatg
     1561 gtgacgatgg cgccctcgat cgccagcatg ccgagcagtt tcgggatcgc aataatctcg
     1621 ttcgattttt ccgccacctt ggtttgcccc agcacgagac gctggcgggt ggcgaaggcg
     1681 gaaaccatgt ggattgcctc tttcgcccct ttcttctggt aggagcggcg tgacgtcttg
     1741 ccgtcgatgg caatcacatc cacaggggct ccggtaaacg ctgcgaccca ggaaacaaag
     1801 cagcgctgga atgtttcggc gtcgagcaca gcgaaaatat cgccgaggtg atcatgagcg
     1861 ggcgtgccgt tgcgaaaggg caggaaccgg cgcaggaggc tgagcttctt ctcgccaaag
     1921 cgcgcgatgt cagcgaatgt ctcagcccca gccagcaccg ccagcagaca gagcagcagc
     1981 acttctctta acggatacac gaccttcccc tgttggcggg gatcagccag atcgttgaaa
     2041 taatccagaa aaacaatcgc ttcgcttaac gcctcgcact ccccagcccg tccgtccatc
     2101 gccgccatcc tcaacgcgaa tcagaggagc gctatcgatt cagcgattgg gcgcgttgtc
     2161 acgaacttct tcacccgatt gccctgagac agaatccgac tcgcattcca tcggccagcg
     2221 aaatcgccta ttactcagac aggctcctag tacagcttat ccgaggcatg ttcgctaaag
     2281 agcgtgcggc aaatgggggc gaacttgcct cgggctgtac tagttccggt tttgcgcgc
//
LOCUS       DATMGX010000324         2923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_48578, whole genome shotgun sequence.
ACCESSION   DATMGX010000324 DATMGX010000000
VERSION     DATMGX010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48578"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2923)
                     /locus_tag="VNR65_05230"
     CDS             complement(<1..>2923)
                     /locus_tag="VNR65_05230"
                     /inference="COORDINATES: protein motif:HMM:NF019927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWJ18117.1"
                     /translation="TTGHAIEVRLNAEDPGNGFAPAPGTIERFRLATGPGVRIDTGVA
                     VGDAIPPEFDSMIAKIIAYGQNRREALSRLQRVLRESVVVIQGGASNKAFLLDLLNRP
                     EVQRAEVDIGWLDRLAANDGHCSRRYADVALVQAAIEAYSAELAVEQTQFYASAVRGR
                     PHVRNELGRTVELRYRGHCYSLRICCLGPHQYRVEVDGSRIEALIHPLGRFEYWLTAF
                     GRRFHVVSVVQGVNSRIEVDGVSHSIDRDDGGMVHAPGPSVVVSIAVKAGDTVSVGDT
                     LAVLEAMKMEMAVVAPFSGKVRQVMAIPHVQVDAGVPLMQIEAIADDRTLATTERVVF
                     GESRTANEPGQDNGPRWRQDLEELRHLLLGFDADPTQTARLFAQWSQFNEAPADSDDI
                     RRREDEVLNIFVDICSLFRRKPGVHDPTSGEAPSTEAYLFSYSRMLETHGEGLPPAFM
                     EALRRTLAHYGVQTLDRSPELEESLLWIYKSHQRVERQVAPILALLERRLARVEALAP
                     HADESFRSLLDQIIAVTRGSFPSVSDLARELRYRCFEQALFEQARKQIYAQAEDHLAY
                     LAANPQAADRRERVNALIECPQPLASFFSGRFTAADHGLRQLMLQIMTSRYYRGQSLA
                     NFRTMPVNGHCSISAEYVQEGKRVHVFAAHAAYRRLLEAARAMCSCLEEVPAGDDIVI
                     DLYLWNSGWLADPEVTQQEVNAALDQAGFPRPVQRIVVAVSGPGCGEGMAGMQHFTYQ
                     PSTNIYEEVKLFRGIHPMMAERLHLWRLKNFTVERLPSVEDVYLLHAKANDNPKDERL
                     FAAAEVRDLTPVRDGTGRIVQLPHLERMFAEAVAGIRLFQSRRDPRERLYWNRIFLYV
                     WPVFDLKPDELNDIAHKLALSTDGLGLEQVVVQARVPNPQTGELRDTVIRISAPGDAG
                     LLMTFRPFSKLQLMKPLTEYNQKVVRMRRRGLIYPYEIVRMLTPAREDTRAEFPHGEF
                     IE"
BASE COUNT          585 a          908 c          855 g          575 t
ORIGIN      
        1 actcgataaa ctcgccgtgg gggaattcgg cccgcgtgtc ttcccgggcg ggggttagca
       61 ttctgacgat ttcgtaggga taaatgagtc cccgccggcg cattcggaca accttctggt
      121 tgtactcagt cagcggtttc atcagttgca gcttactgaa cggccggaat gtcataagca
      181 aaccggcgtc gccgggagcc gaaatgcgta tcaccgtatc gcgtaactct ccggtctgcg
      241 gattgggcac acgggcctgg accaccactt gttccaatcc caagccgtct gtcgacaggg
      301 ccagtttgtg agcgatgtcg ttgagttcat ccggcttgag atcgaacacc ggccaaacgt
      361 aaaggaagat ccggttccag tagagccgct cgcggggatc gcgccgcgac tgaaacagcc
      421 ggatgcctgc tactgcttcc gcgaacatgc gctccaagtg cggcagttgc acgatgcggc
      481 cggtcccgtc gcgcaccggc gtcaaatcac gcacttccgc agccgcgaac agccgctcat
      541 ctttggggtt atcgttcgcc ttggcatgca ggaggtaaac atcttccacc gacggcagcc
      601 gctcaacggt aaagttcttc aagcgccaca gatgtagccg ttccgccatc atcgggtgga
      661 ttccgcgaaa gagctttacc tcttcataaa tattcgtgga aggctgataa gtgaagtgtt
      721 gcatgccggc catgccctca ccgcaacctg gtccagagac tgccactacg atgcgctgaa
      781 cgggccgggg aaaaccagcc tggtcaagcg cggcgttaac ttcctgctgg gtgacctccg
      841 gatcggccag ccaaccggaa ttccacaggt acagatcgat gacaatgtca tcccccgccg
      901 gcacttcctc caggcaagag cacatcgccc gtgccgcttc caagagccgt cgatacgcgg
      961 catgcgcggc gaagacgtgg acgcgctttc cctcctgcac gtattccgcc gatatactgc
     1021 agtgcccgtt taccggcatg gtgcgaaagt ttgcgagcga ttgtccgcga taatatcgag
     1081 acgtcattat ttgcaacatc aattgacgca agccatgatc ggcggcggta aagcggccgg
     1141 agaagaagct cgccagcggt tgcggacatt cgatcaacgc atttactctc tcgcgccggt
     1201 ccgcggcctg cggattggcg gcgagatagg cgaggtgatc ttcggcttgg gcgtagatct
     1261 gtttacgtgc ctgctcaaac agcgcctgct cgaagcagcg gtagcgcagt tcgcgggcga
     1321 ggtcgctgac ggagggaaag gacccgcggg tgacggcaat gatctggtca agcagggaac
     1381 ggaacgactc gtccgcgtgc ggcgccaagg cttcgacgcg tgccagacgc cgctccagca
     1441 aggccagaat aggcgccact tgccgctcca cccgttggtg cgacttgtag atccagagca
     1501 aactctcttc cagttcgggc gagcggtcca gcgtctgtac gccatagtga gctagagtgc
     1561 ggcggagcgc ctccataaac gcaggcggaa gtccctcgcc gtgcgtttcg agcatgcgcg
     1621 agtaggaaaa gaggtaagct tcggtgctgg gcgcttcacc gctggtggga tcgtgcacgc
     1681 ccggcttccg gcgaaacaaa gagcatatgt ccacaaaaat attgaggacc tcatcttcgc
     1741 ggcgtcttat gtcgtcgctg tccgctggag cttcattgaa ctggctccat tgcgcgaaca
     1801 gccgcgccgt ctgggtcggg tcggcatcga agcccagcag gagatgacgc agttcttcca
     1861 ggtcctggcg ccagcgaggt ccgttgtcct gcccgggttc gttcgcagtg cgcgactcgc
     1921 caaataccac ccgctccgtt gttgccaacg tccggtcatc cgcgatggct tcaatttgca
     1981 tcagtggaac tccggcgtct acctgcacgt ggggtatcgc catcacctgc cggacttttc
     2041 cggaaaatgg ggcgacgacc gccatctcca ttttcatggc ttccagcaca gccagggtgt
     2101 ccccaaccga aaccgtatcg cccgctttca cggcaatgga aacaactacc gacggccccg
     2161 gcgcgtgcac cattccgccg tcatcccgat caatcgagtg cgacacgccg tccacttcga
     2221 tccgggaatt tactccttgc accacggaga cgacgtggaa gcgccgcccg aaggcagtaa
     2281 gccaatattc aaaccggccc agagggtgga tgagggcttc gatgcgcgag ccatccactt
     2341 ctacacggta ctggtggggg ccgaggcaac aaatcctgag agagtaacaa tgtcctcgat
     2401 agcgtagttc caccgtccgt cccagctcat tgcgaacgtg gggtcgcccg cggacggctg
     2461 acgcatagaa ctgcgtctgc tccaccgcca actcggcaga ataggcttct atcgccgcct
     2521 gcaccagggc cacatcggcg taacgtctgg aacaatgccc gtcgttcgcc gccagacggt
     2581 ccagccatcc gatatctact tccgcgcgct gtacctcggg gcggttcagc aagtccagca
     2641 ggaacgcctt gttactggct ccgccctgga tgacgacgac gctctcacgt agcacccgtt
     2701 gtagcctcga aagtgcttcc cttcggtttt gcccataggc gatgatcttg gcgatcatgg
     2761 agtcgaactc cggcgggatg gcatcgccta ccgctacacc ggtgtcaatg cgcacccccg
     2821 ggccggtcgc aagcctgaac cgctcaatcg taccgggagc cggagcgaaa ccgtttcccg
     2881 gatcttcggc attcagccgc acttcgatgg cgtgaccggt ggt
//
LOCUS       DATMGX010000325         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_329280, whole genome shotgun sequence.
ACCESSION   DATMGX010000325 DATMGX010000000
VERSION     DATMGX010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329280"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1165
                     /locus_tag="VNR65_05235"
     CDS             248..1165
                     /locus_tag="VNR65_05235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ18118.1"
                     /translation="MTETPRTQTARPSLPSQEGRTAIVTGATGGIGLWTAIGLAKARA
                     QVILVCRDPHRGEDARALVSRMGGRKPDVVVADFANLKTVRDAAAKILDRYPQIHILV
                     NNAGLFSPRRELTVDGYEMTIAVNHLAPFLLTNTLLPALERAGEYGRNARIVNVSSEA
                     ARRASIDLGDLMLSRRYKMMAAYSQSKLANILFTKELGRRLPPRLVSANCLHPGLVGT
                     GIGNKGGVFGFGWSVMKPVMLSPERGAENSLFVATSPTIEGVSGAYFVKQRQAATNPI
                     ADDRAIASRLWAESQKLVDAALSKARVAA"
     gene            1329..>2568
                     /locus_tag="VNR65_05240"
     CDS             1329..>2568
                     /locus_tag="VNR65_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ18119.1"
                     /translation="MAIAATAQDSLSAQDHQKQLYRAVIAATIGTTIEWYDFFLYSIV
                     TGLVFAKLYFPNSEPLAGTLQAFGIYAVGFAARPVGAAIFGHYGDRMGRKAALIATLL
                     LMGLATFAVAFVPAYDQIGIWGAVILTVLRFIQGVGVGGEWGGSVLLSMEWARTTKNR
                     GLIASWPQFGVPAGLFLANLAVLAFSAISGDQFLVWGWRAPFMISIVLVAIGLWIRLG
                     ILETPVFAKLVAEKKVSRAPSVEVLGRYPKTVILSALVRMAEQTPFYLFTAFVFTYGT
                     TVLGTSRDLILIAVLTASFLSFFTIPFSGYLSDRIGRRRMYMIGAACVGIYGFIYFAL
                     LNTNAPAWIFLAIVLSLLPHAMMYGPQAALIAESFPPRLRYSGASLGYQLASIIAGGP
                     APLVATWLFARYNSGYAIAFY"
BASE COUNT          468 a          811 c          737 g          552 t
ORIGIN      
        1 ctctctcatc gtgtcggata gcggaatcag cagcgaaacc gatgattaca gcgattttgt
       61 catcctgact cggtagatca acccgccttt aatcggaatc gactagaggg ggaaaagttg
      121 atcgtttcta aatgttacag cagccagcga cgcgggctgc tgtaggcaca cggaactatt
      181 tataggagcc gcggcgacaa aaccgcggcc ttattgttac tctcgcgagc gcactccgga
      241 ggcaacaatg accgagaccc ctcgaacgca aaccgccagg ccctctttgc cgagccagga
      301 agggcgaacg gccatcgtaa ccggcgcaac cggcgggatt gggctttgga ccgccattgg
      361 cctcgccaag gcgcgcgctc aggtgatctt ggtttgccgc gatccgcatc gaggcgagga
      421 tgctcgcgct ctggtgtcaa gaatgggcgg ccggaagccc gacgtggttg tcgccgattt
      481 cgcaaacctg aagacggtgc gtgatgcggc cgcgaaaatc ctcgaccgct atccgcagat
      541 ccacatcctc gtcaacaatg ccgggctttt ctccccgcgg cgcgaactca cggtggatgg
      601 ctatgaaatg accatcgccg tcaatcacct ggcgccgttt ctgcttacaa acacgctcct
      661 gcctgcgctg gaacgggccg gcgaatacgg ccggaatgcc cggatcgtga atgtgtcatc
      721 ggaggccgca aggcgcgcct cgatcgatct cggcgatctc atgctctcgc ggcgctacaa
      781 gatgatggct gcctactcgc agtcgaagct cgccaatatt ctcttcacaa aagaattggg
      841 ccgccgcctg ccgccccggc tcgtatccgc gaactgcctt caccctggcc ttgtggggac
      901 tggcatcgga aacaaaggag gcgtattcgg cttcggctgg tcggtgatga agcccgttat
      961 gctgtcaccc gagcgaggcg cggaaaactc gctcttcgtg gcaacctcgc ccacgatcga
     1021 aggcgtcagc ggcgcttatt tcgtcaagca aagacaggcc gcgaccaatc ccatcgctga
     1081 cgatcgcgct attgccagcc gcctttgggc ggaaagccag aagcttgtgg atgcggccct
     1141 gagcaaagcg cgcgtcgcgg cctgatccat ccaagaagct accgaccaca cagaagtaaa
     1201 gctgtacgga acctccccgg acgcgccgca tttctcctga agcggcgctg tgccgccaaa
     1261 ataaaatgga aacgccatcg cttcttgccc ccatccgtcc ggcgggaagt tttcccggga
     1321 ggtcactcat ggccatcgcc gctaccgcgc aggattcgct ctcagcgcaa gatcatcaaa
     1381 aacagctcta ccgtgccgtt atcgccgcca cgatcggaac gacgatcgag tggtatgact
     1441 tcttccttta cagcatcgtg acgggccttg tatttgcgaa gctttatttc ccgaattcag
     1501 agccgctggc cggcactttg caagccttcg gcatctacgc cgtcgggttc gcggcacggc
     1561 ctgtcggcgc ggcgatcttc gggcattacg gcgaccgtat gggccgaaag gccgcgctga
     1621 ttgccacttt gctcttgatg gggctggcga ccttcgccgt tgccttcgtt ccagcctatg
     1681 accagatcgg catctggggc gcggtcatcc tcacggtgtt gcgctttatc cagggcgtag
     1741 gcgtgggcgg cgaatggggc ggctctgtcc tgctttcgat ggaatgggcg aggacgacga
     1801 agaatcgcgg cctcattgcc tcctggcccc agtttggcgt tcccgccggt ttgtttctgg
     1861 ccaacctcgc ggtgctggcg ttcagcgcca tttctggcga tcaattcctg gtctggggct
     1921 ggcgtgcgcc cttcatgatc agcattgtgc tggtcgcgat cggcctttgg ataaggctcg
     1981 gcattctgga gacaccggtc tttgcgaagc tcgtcgcgga gaagaaagtt tcgcgggctc
     2041 cttccgtcga ggtcttgggg cgctatccga aaacagtcat cctgtcggcg ctcgtgcgga
     2101 tggcggaaca gaccccgttt tatctcttca ccgctttcgt cttcacctac ggcacgaccg
     2161 tgctcggaac gtcccgcgat ctgatcctca tcgccgtgct tacagcatcg tttctctcct
     2221 tcttcaccat cccattcagc ggctatctct ctgaccgcat cggaagaagg cgcatgtaca
     2281 tgattggagc ggcctgcgtt ggcatctacg gcttcatcta cttcgcgctg ctcaacacca
     2341 atgcgccggc ctggatcttt ctcgccatcg ttctgtcgct ccttccgcac gccatgatgt
     2401 atgggccgca ggcggcgctg atcgcggaaa gcttcccgcc ccggctgcgc tatagcggcg
     2461 cgtctctcgg ctaccaactc gcgtccatca tagcaggcgg ccccgctccg ctggtcgcca
     2521 cgtggctctt cgcgcgctac aattcgggct acgccatcgc cttttaca
//
LOCUS       DATMGX010000326         3231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_365847, whole genome shotgun sequence.
ACCESSION   DATMGX010000326 DATMGX010000000
VERSION     DATMGX010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365847"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VNR65_05245"
     CDS             complement(<1..399)
                     /locus_tag="VNR65_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18120.1"
                     /translation="MSRVTFLIIIAAMIAGAIGFGIAVYAVPLEEIVQFRALINAGLG
                     AISTTNHPDDSNTGIEHPKAQRSKAPRELDTPTQTRRDTDSKCDSRSAKCLGGSPTVA
                     PTPTDIPNRDVSTPRSGSGGELQAPAPENGP"
     gene            complement(577..>3231)
                     /gene="mgtA"
                     /locus_tag="VNR65_05250"
     CDS             complement(577..>3231)
                     /gene="mgtA"
                     /locus_tag="VNR65_05250"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HWJ18121.1"
                     /translation="ALWLTHRFLLWKKFNAKSNAPLDDPAWWLKPLSGDGAGLATDAA
                     GLTSAEARSRLAEFGPNVFHDRQQQSLFVQFLSRFKNPLVVLLLVASAISALTGEVTN
                     FLIISALVLFSVTLDFVQEHRAGKAAESLRQSVSVRATVVRDGKPLETAVTEVVPGDL
                     ALLSAGDMIPADGLVLEARDLFVKQALLTGEPYPVEKRPGALSTGAIDLQDAANAVFM
                     GTSVISGSARMRVVKTGANTAIGAIADSISRRPPPSAFEIGTHRFGLLIMRLTILLVL
                     FVLLANALLHKPWLESFLFAVALAVGLTPELLPMVVSVTLSRGALHMATKRVIVKRLA
                     SIQNLGSMDVLCTDKTGTLTEAKIKLEQHVDAEGKPSDHVLELAYLNSFFETGLKSPL
                     DDAILDHKNIDVSAWKKIDEVPFDFERRRVSVLVEKGNDRLLVVKGASEEIIALCNRY
                     EEQRNEVQPPLDAPALGRLNGQRVALEKEGFRVLGIAWRRVPLDHPHAVVGDEAELVF
                     AGFAGFLDPPKESAGAALAALAESGVTVKIVTGDSELVTQHVCAALNIAVKGVLTGKE
                     IGGMDDSALRVRVEQANLFCRVNPSQKDRVILALKARGHVVGYLGDGINDAPSLHSAD
                     VGISVDSAVDVAKEAADMILLDQDLHVLHDGVLEGRRTFANIMKYIMMGTSSNFGNMF
                     SMAGAALFLPFLPMLPTQILLNNILYDISEVPIPLDEVDAEDVRSPQVLDMSFIRNFM
                     LVIGPISSAFDFLTFYVMLVVLRADEAMFQSGWFVESLSTQVLVIFIIRTRGNPFKSR
                     AHPVLVATSLAIAAMGALLPFTPLGPYFGFVPLPGRFYLILTAMVVAYLAIVEVAKRG
                     FYRWLGGSGGSKAADLVRLSVRDRDAHF"
BASE COUNT          703 a          958 c          922 g          648 t
ORIGIN      
        1 aggcccattc tccggcgcgg gcgcttgtag ctcgccgcca cttccactcc gcggtgtgct
       61 gacgtcgcgg ttaggaatgt ccgttggagt gggagccact gtgggagacc cgcccaggca
      121 tttcgcgctg cgcgaatcac attttgaatc ggtgtctctc cgggtttggg ttggtgtgtc
      181 gagttctctc ggcgccttcg accgttgtgc ttttggatgt tcaatcccgg tattgctgtc
      241 atctggatga ttggtcgtgg agatcgcgcc gagcccggcg ttaatcagag cacggaattg
      301 gacgatctct tcgagcggca ccgcataaac ggctattcca aatccgatcg ccccggcgat
      361 catcgcggcg atgatgatca ggaaagtgac gcgcgacatt ccatgaaccc ccaaaaacga
      421 attgtcccgc atttcggacg ggcgcgtctt tcctgcactt gcgttctaag cgtcgtccat
      481 tttcgcttcg atgcgaatgt atcgttataa gctcaatatt gctttcgatg aaactgatcg
      541 tgcgacctaa acgcctcaaa tgtgaagaac gaccgcttag aaatgggcgt ccctatcgcg
      601 aacgctaagc cggaccaaat cggcggcctt tgaaccgcca gatcctccga gccaacggta
      661 gaaacctctc ttggcgacct cgacaatcgc cagataggca accaccatcg ccgttagtat
      721 gagatagaat cggccgggta gcgggacaaa cccgaaatac ggtcccaaag gggtaaaggg
      781 cagtaacgcg cccatcgccg cgatagcgag ggacgtcgcg accaagacgg ggtgcgcgcg
      841 gcttttgaag ggattgccgc gagtgcggat aatgaagatg accaagactt gggtcgacag
      901 tgattcgaca aaccagccgc tctggaacat cgcctcgtcc gccctcagca cgaccaacat
      961 gacgtagaaa gtcagaaagt cgaacgccga actgatcggc ccgattacga gcataaaatt
     1021 gcgaataaag ctcatgtcca aaacttgcgg actgcgcacg tcctcggcgt ctacttcgtc
     1081 cagagggatc ggcacttcgg aaatgtcata gagaatgttg ttgagcagga tctgagttgg
     1141 cagcatcggc agaaagggga gaaacagagc ggcgccggcc atgctgaaca tgttgccgaa
     1201 gttagagctg gtgcccatca ttatgtattt catgatattc gcgaaggtgc ggcgaccttc
     1261 cagcacgcca tcgtgaagca catgcagatc ctgatccagg aggatcatgt cggccgcttc
     1321 cttcgcgacg tcgacggctg agtccaccga tatgcccaca tcggctgaat gcagcgacgg
     1381 cgcatcgttg atcccgtcgc ccagataccc gacgacatga ccgcgcgctt tgagcgcgag
     1441 gatcacccga tctttctgcg acgggttgac ccggcaaaac agattggctt gttccaccct
     1501 gacccgcaag gcggagtcat ccatcccccc gatttctttc cccgtcaaca cgcctttcac
     1561 cgctatattc aaggccgcgc agacgtgctg ggtgactaat tcgctatcgc cggtgacgat
     1621 cttcaccgtc actccgcttt cggcaagcgc cgcgagcgcc gccccggcgc tctccttagg
     1681 cggatcaaga aaccccgcga aacccgcgaa cactagttcc gcctcgtcgc ccacaacggc
     1741 atggggatgg tccagtggaa cccgccgcca agcgatgccc aagacgcgga agccttcctt
     1801 ttcgagggcg acgcgctgcc cgtttaaccg gccaagggcg ggagcatcga gaggaggctg
     1861 gacctcattc cgctgttctt cataccggtt acaaagagcg atgatctcct cggacgctcc
     1921 cttgaccacc agcaatcggt cgttaccttt ctccacgaga acggacacgc gacgccgttc
     1981 aaagtcgaat gggacttcgt cgatcttttt ccacgcgctc acgtcaatgt ttttgtgatc
     2041 gaggatcgcg tcgtcgagag ggctcttcag gccggtctcg aaaaagctat tgagataggc
     2101 gagttccagc acatggtcgc tgggcttccc ttccgcgtca acatgctgtt ccagctttat
     2161 cttcgcctcg gtcaacgtgc cggtcttgtc cgtgcacaag acgtccatcg atccaagatt
     2221 ctggattgag gccaaccgct tgacgattac gcgcttcgtc gccatgtgca gcgcgcctcg
     2281 ggacaaggtg acggacacca ccatcggcag cagttccggg gtcaggccca cggcaagcgc
     2341 gacggcaaac agaaaggatt cgagccatgg cttgtgcagg agtgcattcg ccaatagcac
     2401 gaacagcacc agcaagatcg tgaggcgcat gataagcagg ccgaagcgat gcgtgccgat
     2461 ttcgaaggcg gatggcggcg gtcggcgact aatgctgtcg gcgatcgctc cgatggccgt
     2521 attggcgccc gttttcacca cgcgcattct ggcgctgcca ctgatgacgc ttgtgcccat
     2581 gaacacagcg ttggcggcgt cctgtagatc gatcgcgccg gtcgatagcg cgccgggacg
     2641 tttctcgaca ggatagggct cgcccgtcaa cagcgcctgc ttgacgaaca aatcgcgcgc
     2701 ttccagcacc aagccatctg cagggatcat atcgcccgcg gacagcagag cgaggtcgcc
     2761 gggaaccact tccgtgaccg ccgtctccaa gggcttgcca tcgcgcacca ccgtggctcg
     2821 caccgatacc gactggcgca agctctccgc tgcttttccg gcgcggtgct cttggacgaa
     2881 atccagcgtc acgctgaaca gcaccaacgc cgagatgatc aaaaagttag tgacttcgcc
     2941 tgtgagggcg gaaatcgcgc tggcgacgag tagtagaacg acaagaggat tcttgaaacg
     3001 ggaaagaaat tgcacaaaca gcgactgttg ttgacggtcg tggaacacgt tggggccaaa
     3061 ttcagcgaga cgcgagcgcg cttcggcgct tgtcaagcca gccgcatcgg tcgctaaccc
     3121 cgctccgtcg cccgaaagcg gttttagcca ccaagcggga tcgtctagag gggcattaga
     3181 cttagcgttg aactttttcc agagaagaaa tcgatgcgtc aaccacaggg c
//
LOCUS       DATMGX010000327         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_297045, whole genome shotgun sequence.
ACCESSION   DATMGX010000327 DATMGX010000000
VERSION     DATMGX010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297045"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VNR65_05255"
     CDS             complement(<1..493)
                     /locus_tag="VNR65_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWJ18122.1"
                     /translation="MTPHDANAFDYVIVGAGSAGCVLAHRLSASGRHRVLLLEAGGED
                     RSVWIHVPLGFGKLFTNPQVNWLYMSEPEPECGDRRIIQPRGKVLGGSSSINGLLYVR
                     GQAEDYDRWRQLGNPGWSFADVLPYFRKAEDQQRGSSDFHGVNGPLAVSDTEAHPLCE
                     AFLE"
     gene            complement(498..629)
                     /locus_tag="VNR65_05260"
     CDS             complement(498..629)
                     /locus_tag="VNR65_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18123.1"
                     /translation="MKTRIHSKGGNPFTSTPAQYAADIDRMETKLATLIRKLGSKVE"
     gene            complement(880..1368)
                     /locus_tag="VNR65_05265"
     CDS             complement(880..1368)
                     /locus_tag="VNR65_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18124.1"
                     /translation="MKPNQQANYNHTFRAIYDDEIPWQEGSKSVLMLPDGVQVKIFAH
                     DRAMGRIDMKVKFPPGYVEPEHAHKSWHSIVVLKGRMCVAGKDLRPGDYVFGWDELHG
                     PYEYPDGCEVFVVFMGESVAHEWDEGRHKAHQNVWRAETEEGRQGIDQHIARRKLGQK
                     IC"
     gene            complement(1573..1749)
                     /locus_tag="VNR65_05270"
                     /pseudo
     CDS             complement(1573..1749)
                     /locus_tag="VNR65_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1919..2530)
                     /locus_tag="VNR65_05275"
     CDS             complement(1919..2530)
                     /locus_tag="VNR65_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ18125.1"
                     /translation="MLGLLGRNGVGKTTLVNSIVGFNPPRRGKIVFKGDDITQKSSFE
                     TVRSGMGLVPQGRRVFPTLGVEENLLVAERNPDRHGWNLDRVYKLFPRLHERRNQRAK
                     TLSGGEQQMLAIGRALMTNPDCLIMDEPSEGLAPIIIQGLWEAIGKLKEEGLSILLIE
                     QSAHLALKLVDYVHVMSKGQVVYSGKPDELKANDEIKSSFLGI"
     gene            complement(2624..>2912)
                     /gene="livG"
                     /locus_tag="VNR65_05280"
     CDS             complement(2624..>2912)
                     /gene="livG"
                     /locus_tag="VNR65_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Part of the ABC transporter complexes LivFGHMJ and
                     LivFGHMK involved in the high-affinity transport of
                     branched-chain amino acids; LivFGHMK is specific for the
                     transport of leucine, while LivFGHMJ is a transporter for
                     leucine, isoleucine, and valine; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HWJ18126.1"
                     /translation="QRQLEIVLGLASDPKILLLDEPAAGLSSGESTEMTKFLMKLDPK
                     LAILLIEHDMDVVFDVAAQISVLHFGEVLETGTPEQIHDSKRVQEIYLGTD"
BASE COUNT          561 a          863 c          840 g          648 t
ORIGIN      
        1 cttccaggaa ggcttcacac aacggatgtg cctcggtatc ggacacggcc aagggaccgt
       61 tgacgccgtg aaagtcggac gagccacgct gctgatcctc cgccttgcgg aagtagggca
      121 gtacatcggc gaagctccag cctggattgc cgagctggcg ccagcgatca tagtcctccg
      181 cttgcccacg cacatagagt aggccgttga tcgagcttga tccgcccaac acttttccgc
      241 gcggctggat gatccggcgg tccccgcatt cgggctccgg ctccgacatg tagagccaat
      301 tgacctgcgg attggtgaaa agcttgccga agccgagcgg aacatggatc cagacgctgc
      361 gatcctcgcc gcccgcctcg agcagcagca cgcgatggcg tcctgacgcg cttagccgat
      421 gggcgaggac gcaacccgcg ctgccggcgc cgacgataac gtaatcgaaa gcgttggcgt
      481 cgtgtggagt caccgcctca ctccaccttt gagccgagct tgcggatcag ggtagccaac
      541 ttggtctcca tgcggtcgat gtcggcggcg tattgggcgg gtgtgctggt aaacggattg
      601 ccgcctttgc tgtggatgcg cgtcttcacg tcgcccgccg ctgctagctc gtccggttcg
      661 tagactgctt tgtgtttctg gaatctctac attttggaaa gtggtccgtg tggaaacatg
      721 ctataaacaa atggatcatc cttggttacg attattcctt ctttcagccg aaaccctaca
      781 tctgaacatg ggcgcccgcg acagaggcgg ctgggccagt cccgaggtag cgtgcgtcac
      841 cggccacacg ctcgctctgg acggcgggct gacgacgtat tagcagattt tttgacctag
      901 cttgcgccgc gctatgtgct ggtcgatgcc ttgtcggcct tcctcagtct ctgccctcca
      961 gacgttttgg tgagccttgt gtctcccttc gtcccattca tgcgcaacac tttcacccat
     1021 gaacacgacg aagacctcgc agccatcggg gtactcgtat ggtccgtgca attcgtccca
     1081 gccgaagacg tagtcgccag gacgaagatc cttaccagcc acgcacatgc gccccttgag
     1141 cacgacaatg gagtgccagc tcttgtgagc atgctccggc tcgacatagc cggggggaaa
     1201 tttgaccttc atgtcgatcc gccccatcgc cctgtcatgg gcgaagattt tgacctgcac
     1261 gccgtccggc agcatcagta cggattttga tccttcctgc catggaattt catcatcgta
     1321 gatggcacga aaggtgtggt tatagttcgc ttgctgattc ggcttcattg tttgttcctg
     1381 gaaactaaac ttgctgccgc tctctaaccg aaattcccca gatgaactct ggaacggcct
     1441 agatgatagc agacggtggg ggttcggcgg aagccatagg cgagctgcac attgctcccg
     1501 gcgttgaatg tgcagtcaaa ggaccagtta cggtaattcc gttaggaata tttccgcagt
     1561 tatatacttg acttagcaga tcgcccgcgc cgtatgggcg cattgatcgt agcgggtatg
     1621 aacgcgtcgc cagtccttga gcctgccgaa catattctcg atcttgtggc gctgtcggta
     1681 aagcgtgcgg tcatgttcga tcggcgcctt gcgatttgat tttgacggga tgcacgccgt
     1741 gatgccgcgc tgaggtgtcc cgtataagca aaatcatgag ccatcgaaca ttccagttcg
     1801 ctgccgttgg ccgcctagaa gccatataag ggtgccgcaa catcgaacgg atcagggcgg
     1861 cagaaaccgc tccgacgaga ccggatgacc ccataaaaac tcgccgtgat tccgtcgatt
     1921 agatgcctaa aaagctcgat ttgatctcgt cgttggcctt gagttcgtcc ggcttgcccg
     1981 aataaaccac ctggccctta ctcatgacgt gcacgtagtc gaccagcttg agcgcgagat
     2041 gcgcgctctg ctcgattagc aggatcgaca ggccttcttc cttcagtttg ccgatcgctt
     2101 cccacaggcc ctggatgatg atcggcgcca gcccttccga cggctcgtcc atgatcagac
     2161 aatcggggtt ggtcatcagc gcgcggccga tcgccagcat ctgctgctcg ccgcctgaca
     2221 acgtcttggc gcgctgattg cgccgctcgt gcaggcgcgg gaacagtttg tagacgcggt
     2281 cgagattcca gccgtggcgg tcggggttgc gctcggccac cagcagattt tcctccacgc
     2341 cgagcgtcgg aaacacgcgg cggccctgcg gcaccaggcc catgccgctg cgcacggtct
     2401 cgaacgagga tttctgggtg atgtcgtcgc ccttgaacac gatcttgccg cggcgcggcg
     2461 gattgaagcc gacgatcgaa ttcaccagcg tggtcttgcc gacgccgttc ctgcccagca
     2521 gcccgaggat ctggccctac tcaagttgca gcgacagccc ctggagaaca taggcgtcgc
     2581 cgtaataggt gtgaatgtcc tggacttcga gaatcgccat gactcaatcg gtccccagat
     2641 agatttcctg gactcttttg ctgtcgtgga tttgctcggg cgtgccggtt tccagaactt
     2701 cgccgaagtg cagaaccgag atttgcgcag cgacgtcgaa cacgacgtcc atgtcatgtt
     2761 cgatcaagag gatcgcgagc ttcgggtcga gtttcatcaa gaacttggtc atttccgtcg
     2821 attcacccga tgagaggccg gcggccggct cgtcgagcaa aagaatcttc ggatcgctgg
     2881 cgagccccag cacgatctcg agctgacgct gc
//
LOCUS       DATMGX010000328         2422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_474388, whole genome shotgun sequence.
ACCESSION   DATMGX010000328 DATMGX010000000
VERSION     DATMGX010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474388"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /gene="guaA"
                     /locus_tag="VNR65_05285"
     CDS             <1..840
                     /gene="guaA"
                     /locus_tag="VNR65_05285"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWJ18127.1"
                     /translation="AIGDQLTCIFVNNGVLRKNEFVTVQKNLRDKLGLNIDAVDATDL
                     FLGKLAGVTDPETKRKIIGNEFITVFDQEAHRIEQLHGKVDWLVQGTLYPDVIESQSV
                     RGPSQTIKSHHNVGGLPDKMKLMLIEPLRDLFKDEVRKIGRDLGMPEDILQRQPFPGP
                     GLAVRILGEVTPKRVALLQEADDIVVGEIKKAGLYTKIWQSFAVLLPVMSVGVMGDMR
                     TYADTCAIRAVNSEDGMTADWVDLPHEVLKTISNRIVNEVKGINRVVYDITSKPPGTI
                     EWE"
     gene            complement(1195..1968)
                     /locus_tag="VNR65_05290"
                     /pseudo
     CDS             complement(1195..1968)
                     /locus_tag="VNR65_05290"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1984..2184)
                     /locus_tag="VNR65_05295"
     CDS             complement(1984..2184)
                     /locus_tag="VNR65_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18128.1"
                     /translation="MDAATGETGECKLVNGDGEAERFYRSMAEPALVGVEACGNSQWF
                     VELLQQLGHEVWIGDAAQIRAS"
BASE COUNT          453 a          841 c          644 g          484 t
ORIGIN      
        1 gccattggcg accagttgac gtgcatcttc gtcaacaacg gggtgctgcg caagaacgaa
       61 ttcgtcaccg tacagaagaa cctgcgcgac aagctcggcc tcaacatcga cgccgttgac
      121 gccaccgacc tcttcctcgg caaactcgcg ggtgtcaccg atcccgaaac caaacgcaag
      181 atcatcggca acgagttcat caccgtcttc gatcaggagg cgcaccgcat cgagcaactg
      241 cacggcaaag tcgattggct ggtgcagggc acgctctatc ccgacgtgat cgaatcgcaa
      301 tcggtgcgcg gcccttcgca gaccatcaaa agccatcaca acgtcggcgg cctgcccgac
      361 aagatgaagc tcatgctcat cgagcccctg cgcgacctct tcaaagacga agtccgcaag
      421 atcggccgcg acctcggcat gcccgaagat atcctgcagc gccagccttt ccctgggccc
      481 ggactggcgg tgcgcattct cggcgaagtc acccccaagc gcgtggccct gttgcaggag
      541 gccgacgaca tcgtggtcgg cgagatcaag aaggcggggc tctacaccaa gatctggcag
      601 tcgttcgccg tgctgctgcc ggtgatgtcc gtcggcgtga tgggcgacat gcgcacctac
      661 gccgacacct gcgccatccg cgccgtcaac tccgaagacg gcatgaccgc cgactgggtt
      721 gacctcccgc acgaagtcct gaagaccatc tccaaccgca ttgtcaacga agtcaaaggc
      781 atcaaccgcg tggtgtacga cataacctcc aagcctccgg gaacgattga gtgggagtga
      841 ggaaggtagc ggcaagggtc ggcgtcctca gaattgacga gtcttccccg tactcgctcc
      901 aaccctgcga tctcggtggg ttggacaagg tgatgactat ttgcgggtgc ccctcgataa
      961 ggtcgaaggc gttgtcaagc gatagttctc ctgagcggtg cgcgagcacc gcttttcttg
     1021 ccactgtcgg ttcggttgag gaccaggaac actggagaac tcgcgttaat cattgtccgg
     1081 tgaaaccagc ttcgttccac caaccatcga tacggccatt cactgcggcc atgattcttc
     1141 tatgcgaacc tcccgtctct acgggaggcg agagcgccca atcagttcat cggtctagct
     1201 tgcgccgacc aggggcaccc gcgggctgct ctcgatgcga acgatctccg gataccccgt
     1261 gttggtgcgc agcatccagt agagtcgtac cgccaacttg cgtgcggctg ccaccttggc
     1321 cacgcctttc gccatgcgat ggcagcgctg ctgatacgtt ttgcgaaacc cctcatccag
     1381 ccgattggcg ctgtgggctg cttccaccag cagcatgcgc acaaatcggt tgccctgctt
     1441 gctgatcgcg cccagccgct gcttgccgcc cgagctgtgc tcgcgcggaa tcagccccag
     1501 attgctggcc acctgcttgc cccgccgaaa gcgcgagacc tcgccaatgg tcaccacgaa
     1561 cgccagcgcc gtgatcggac ccacccccgg ctgcgtcatc aacagttgcg ccattttgtc
     1621 tctctgagcg gtttcttcca ccgcctggtc cagttgcccg atctgctcgt ccaggctctt
     1681 cagcaacccc agcaaatcct gtcgccgctg cgccgcccag ccttgcaacg gcaactcgcg
     1741 gaacgctttc tgtccggccg cgctccacag cttgctcttc ttctgcatcc cccggttcat
     1801 cgccagatgc tgcaatccgt tcttcacccg cgttcgtatc tccaccagct tgtgccgatg
     1861 gatcagcaac tgccgcacgt cccgctgctc tgccgacggc atccacagcc gcggaaaccg
     1921 gttctccaac agcaaccgca aaatgtgcgc cgcgtcccgc ttgtccgtct tctgcttgcg
     1981 tacctagctg gcccggatct gcgctgcatc cccgatccac acttcgtgtc ctaactgctg
     2041 caacaactcc acaaaccact gactgttgcc gcaggcttcc acccccacca gcgccggctc
     2101 cgccatgctg cgatagaacc gctcggcctc cccgtcgccg ttgaccagct tgcactctcc
     2161 cgtttctccc gtcgcggcgt ccaatcacgc cacctgttgc cagctgggat gaaaatcaca
     2221 tcctataatc ctcatcgaag gctccttccc tccccagcgt ctttcttggt cctctaccgc
     2281 aatagacgtc tcaggatagg cgcgagcctt cgaccttatc caatcagttc tacgactcgc
     2341 gcagcgaggc gtagggcggg gtgctagtcg cttggtgccc cagagcctgc cctgagcttg
     2401 tcgaagggtt ggcgccggtt tc
//
LOCUS       DATMGX010000329         2265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_324385, whole genome shotgun sequence.
ACCESSION   DATMGX010000329 DATMGX010000000
VERSION     DATMGX010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324385"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            544..822
                     /locus_tag="VNR65_05300"
                     /pseudo
     CDS             544..822
                     /locus_tag="VNR65_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1639..>2265)
                     /locus_tag="VNR65_05305"
     CDS             complement(1639..>2265)
                     /locus_tag="VNR65_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWJ18129.1"
                     /translation="CDPSKVIAVVETNAPDRNTTFAPPDQTSKAIAGHILEFLGHEVR
                     RGRGRIVLRTQEISNHPEVIRRLGVIAMNGMIEADIYGNVNSTHIRGTSIMNGIGGSG
                     DFARNAYLSIFMTPSTAKHASISSIVPMVTHVDHTEHDVQIVVTEHGLADLRGLSPKQ
                     RAGVVIDNVAHPSFRPALRDYFERACKFSPGKHTPHLLDEAFRLVQAT"
BASE COUNT          499 a          643 c          677 g          446 t
ORIGIN      
        1 gggcctgttt caccttacaa gaactggccg gtatcaacag actaatagct tgcagataca
       61 acgacgaggc aatcaagagc cgctttgcgc cagaagcggc cggtcgcgcg ttcataaccg
      121 cgcaaaaatg gtcgctcgtc atagaatgaa ccgctcacga aagagcgacg cggtgggcgg
      181 gatggcggag gatcccccat gcctgtacgt tgcgtcagcc ccagtcgtca accttggaac
      241 aaaggattgc tcgtcggcca gaagaagcct ctcgaaccga agcatgtatg gtcgattcga
      301 gttcggttag aaatcgcagg atcaaagcgc gatcttgcaa tctttaatct agcaatcgac
      361 agcaagctgc gcgcttgcga tctggtcaaa ttgcggctgg acgacatttg ctctgggtta
      421 agagtgcggc aacgagcaac gatcgtgcaa aagaagactg gtcgaccagt ccaattcgag
      481 atcacggagc agtcgaggaa ttccgtcgaa gcctagctac catcgctccg ggccggtggt
      541 tctcgatacc tcttcccaag tcggcttcac gcgagccctc acatatcaat taggcaatat
      601 gcccggctgg tgcaccgttg ggtcgaaagc atcggcttgg agcgagctgt ttatggcacg
      661 cattcgatga ggcggacgaa ggccgcccag atttaccgca aaaccggaaa cctccgcgcc
      721 gtgcaacttc tcctcggcca tacaaagctg gagagcaccg tccgatatct cgggattgag
      781 gtagatgacg ctctaaatat cgccgagctg atcgagctct aacagccacc ggtggctatc
      841 gacgaatcgt ggagcgtcat cgccaccatc gctgatatcg cctagcccgc gtcggcgggg
      901 cgtctgcgct actcataatc gcaagactgc aacgctcaag gacgcgcgtt aatctaacgt
      961 ggtatagttt ttgctagttt cgtgttagct tgatggcgcg cgcgctggtt ctcagcgtcc
     1021 gcacattcgt caagccaacc aaccccacga ggaagacatg gcaaagaaag cgaagaaagc
     1081 aaagaagact gccaaggcga ccaagaaagt cgccaagaag accaagaaga tgaagaagta
     1141 agcgcgcggt tttccgcaag ccaaagccgc cgcggtatcg cgaccaccat cgctggtgag
     1201 cttgacgcat cgcaagatgt ctctggccga aaacgcggcg acgatcagac cggccccatg
     1261 tgcacacggc gcaagaagcc tacgactgat gcacatgggt ccggtttttt tgtgtccggt
     1321 cgttggtcgg gtcgcccgtc gcaccggtgt cagtcgattc cccggcttgc gacttagcgc
     1381 ccttcgccca tcccttcgtt gaaggggcgg cgtcggggcg cctccagcgc tcctgcattg
     1441 acgatagcga ccgctgcgtt gcgccagatg ccgccattcc cgcaggttcg attttcggca
     1501 ggtttgagac atgccgaccg ggcgggacga gtccgccccg ggggagatcg gcgatcgacc
     1561 cgactcggaa gtgcgagggc gacttacgag ggcgtaaatc gcaaaagccc gcattgatgc
     1621 agaaagtgcc gtcccaagtt aggttgcttg aactaaacgg aaggcttcgt ccaacagatg
     1681 cggggtgtgc tttccaggtg aaaatttgca cgcccgctca aagtaatccc tgagcgcggg
     1741 ccggaacgat ggatgcgcta cgttgtcaat cacgactccg gcccgctgtt tcggagacag
     1801 accgcgcagg tccgcgaggc catgctccgt caccacgatc tgcacgtcgt gctcggtatg
     1861 atcgacatgg gttaccatcg ggacgataga cgagatcgat gcgtgtttgg cggtcgaggg
     1921 cgtcatgaag atcgagagat aggcgtttcg ggcgaaatcg cccgagccgc cgataccgtt
     1981 catgatgctg gtgccgcgga tgtgggtcga gtttacattg ccgtagatat cggcctcgat
     2041 cattccgttc atggcgatca cgccgagccg ccggatcacc tcaggatggt tggagatttc
     2101 ctgcgtccgc agcacaatgc ggcccctgcc gcggcgaacc tcgtgcccga gaaattcgag
     2161 aatatggccg gcgatcgcct tcgacgtctg gtcgggcggg gcgaaagtcg tgttgcggtc
     2221 cggcgcattg gtttcgacca cggcgatcac cttgcttggg tcgca
//
LOCUS       DATMGX010000330         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_419883, whole genome shotgun sequence.
ACCESSION   DATMGX010000330 DATMGX010000000
VERSION     DATMGX010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419883"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="VNR65_05310"
     CDS             <1..1301
                     /locus_tag="VNR65_05310"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWJ18130.1"
                     /translation="ERLVDAAVVELGLDPIEFRRNNFIRPEQFPYKIPTGNLYDSGNY
                     GAVLDKALSLLDYKGWRKKQEEFRKQGRYIGIGIATCQERSVFSATEFWMLNHEAGFA
                     LTSSPEAVSVKIDPTGKAVVSLQAPFWGNSPETMATQILAEILQLEPADISVTYSDTD
                     HGLAGTGPGGSRYTVMVAGALVGAGQAIKTKMFRVASHMLEASIDDLELREGKVGVKG
                     APGHGKTIAEIALHAHLFRLSMPDEPDLTSGLDAAYTYDHPLTTMPSKDRSDLGIFYP
                     IMGHMCHLPVVEIDVETGKVSFLDYVAVHDCGTMVNPMTLGGHIRGGTAQGIGTALLE
                     QFYYDEDGQLLTASFADYLIPTVHEIPQDVRIGHVETPSPFTEFGIKGGGEGGRMGAP
                     PAICSAIEDALRPLGVKVDSMPMTPSKLRGLIREAQARAS"
     gene            1388..>2242
                     /locus_tag="VNR65_05315"
     CDS             1388..>2242
                     /locus_tag="VNR65_05315"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWJ18131.1"
                     /translation="MKVDKSAVYEAARKLSNWGRWGADDQIGTLNNVTSEDIVGAGRL
                     IRKGKVFALGLSLKEPIQSGLFGGRWNPIHTMLATGTDAKAGNQDEPAPYLRYADDAI
                     NMPCQASTQWDALCHIFLDDKMYNGFDAALVDARGARKLGIEHVRDKMVGRGVLLDVA
                     RFKGVASLDDGYAISIADLNLCAAAQGVEIRKGDFVLVRTGHQERCLAKKDWSGYAGG
                     DAPGFAFETCYWLREKDVAAICSDTWGCEVRPNETNEANQPWHWVVIPAIGISMGEIF
                     YLKDLAEDC"
BASE COUNT          483 a          616 c          685 g          458 t
ORIGIN      
        1 tcgagcggct ggttgacgcc gcggtcgtcg aacttgggct cgatccgatc gagtttcgca
       61 ggaacaattt tatccgcccg gagcagtttc cgtacaagat tccaaccggc aatctgtacg
      121 acagcggcaa ctatggcgcc gtgttggaca aagcgcttag cttgctcgac tacaagggct
      181 ggcgcaagaa gcaggaagag tttcgcaagc agggccgcta tatcggcatc ggaatagcga
      241 cctgtcagga acgaagcgtt ttcagtgcta ccgagttctg gatgctcaat cacgaagccg
      301 gattcgcgct gacaagttct cccgaggccg tgagcgtgaa aatcgatcct acgggtaagg
      361 cggtggtgtc gttgcaggct ccattctggg gcaacagccc cgaaacgatg gcgacgcaga
      421 ttctcgccga aattcttcaa ctcgaaccgg ccgacatcag cgtcacctat tccgatacgg
      481 atcatggtct cgccgggacg ggtccgggag gtagccgcta cacggtcatg gtcgctggtg
      541 ctctcgtcgg agccgggcag gctatcaaga caaaaatgtt cagggtcgca agtcacatgc
      601 ttgaagcatc gattgacgat ctcgaattgc gcgagggaaa ggttggtgtg aagggcgcgc
      661 cgggacacgg caagaccatc gcagagatcg ctctccacgc gcatctgttc cggttgtcga
      721 tgcctgatga gccggatctg acgagcggtc tggatgcggc ctatacttac gaccatccgc
      781 tgaccacaat gccgtccaag gaccggtccg acctcggcat tttctatcct atcatgggcc
      841 atatgtgcca ccttccggtc gtcgagatcg atgtggaaac cggaaaagtc agttttctcg
      901 attacgtcgc ggtccatgat tgcgggacca tggtcaatcc catgacgctg ggcggccata
      961 tccgcggcgg caccgcccag ggaatcggaa cagctctgct tgagcagttt tattatgatg
     1021 aggacggaca gcttctgacc gcctcgttcg cggactatct catcccgacg gtgcacgaaa
     1081 taccacaaga tgttcggata ggtcacgtcg agacaccatc gcccttcacc gaattcggca
     1141 tcaagggtgg gggcgaaggc ggtcggatgg gggcgccgcc agcgatttgc tccgccattg
     1201 aggatgcgct gcggcccctc ggcgtcaagg tggacagcat gccgatgacg ccgagcaagt
     1261 tgcgcggcct catcagggag gcgcaagcgc gcgcctcctg agcgcgacgc cgggtccgac
     1321 ccttgcgtcg ggatagccct aagaaaatca cgatcgatcg ttagcaaatg cgtagacggg
     1381 aacaatcatg aaggtcgaca aatcagcggt ttatgaagcc gcacgtaagc tatcaaactg
     1441 gggacgttgg ggagccgatg atcagatcgg aacgctcaac aatgtgacgt cggaagatat
     1501 cgtcggggcg gggcgcctga tcagaaaggg caaggtcttt gcgcttggcc tttccctgaa
     1561 ggagccgatc cagtccggcc tatttggcgg gcgctggaat cccattcata ccatgctagc
     1621 caccgggacc gacgccaagg ccggtaacca ggacgagccc gcgccctatc ttcgctatgc
     1681 tgacgatgcc atcaacatgc cttgtcaggc atcaacccag tgggacgccc tctgtcacat
     1741 cttccttgat gacaagatgt acaacggctt cgatgccgct cttgtcgatg cccgcggtgc
     1801 caggaagttg ggcattgagc atgttcgcga caagatggtc ggacgaggag tgctgctcga
     1861 tgtcgcccgc ttcaagggcg tcgcgtctct tgacgacgga tatgcgatat caatagccga
     1921 tctcaaccta tgcgcggcgg cccaaggtgt tgaaatccgc aagggcgatt tcgttctggt
     1981 tcgtaccggt catcaggagc ggtgcttggc aaaaaaggat tggagcggat atgcgggcgg
     2041 cgatgctccg gggtttgcat tcgagacgtg ctactggctt cgggaaaaag atgtcgctgc
     2101 gatctgttcc gatacctggg gctgcgaggt gcgccccaat gaaaccaacg aagccaatca
     2161 accctggcat tgggtggtga tccctgcgat cgggatttcc atgggcgaaa tcttctattt
     2221 gaaggacctg gccgaggatt gc
//
LOCUS       DATMGX010000331         2377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_206791, whole genome shotgun sequence.
ACCESSION   DATMGX010000331 DATMGX010000000
VERSION     DATMGX010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206791"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /gene="rpsI"
                     /locus_tag="VNR65_05320"
     CDS             complement(<1..218)
                     /gene="rpsI"
                     /locus_tag="VNR65_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWJ18132.1"
                     /translation="MPETSAKTFSGLDKGAGAPAAEDRSRPVHVQKLDAHGRAYATGK
                     RKNAVARVWVKPGQGKITVNDKDHEAYFA"
     gene            complement(218..685)
                     /gene="rplM"
                     /locus_tag="VNR65_05325"
     CDS             complement(218..685)
                     /gene="rplM"
                     /locus_tag="VNR65_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWJ18133.1"
                     /translation="MKTYSAKPSEIEKKWVLIDAEGLVVGRVATIIANRLRGKHKPTF
                     TPHMDDGDHIVVINAEKIVFTGNKTQDKIYYRHTGHPGGIKERRPFEILGGKFPERVL
                     MKAVERMVPRGPLGRKQMTNLRIYAGPEHPHAAQSPEKLDVPAMNSKNVARAR"
     gene            complement(873..1157)
                     /locus_tag="VNR65_05330"
     CDS             complement(873..1157)
                     /locus_tag="VNR65_05330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWJ18134.1"
                     /translation="MLRPGGTISGPAMFALADVAMYAAVLASIGPVAHAVTTNLTINF
                     LRKPDQRDMLGETRLLKLGKRLAVGEIALCSEGETELAAHATATYSLPHY"
     gene            complement(1167..1409)
                     /locus_tag="VNR65_05335"
     CDS             complement(1167..1409)
                     /locus_tag="VNR65_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18135.1"
                     /translation="MLGNPSARSFDRFAAALIAPNLGQITERMLHDHDVPKLAQPKLS
                     AAEVNLFLAEVFPQIDIGRVHKVEETRHGFARMRMV"
     gene            1408..1986
                     /locus_tag="VNR65_05340"
     CDS             1408..1986
                     /locus_tag="VNR65_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18136.1"
                     /translation="MADFKKSAGLILLGLMAAVLSAIGPVAAKDVALTQDNVSRFLAS
                     FAEMRAIAMIEGVRTGMDAETSKNPVGVIVKAIKSSKLQAQAQGIAVKHGFADIREWS
                     DTGRAIGQAYLYVTAGPARGIARETLNKNKDTAIKELEKLGLLNDKQKERLRENLDDL
                     SDQLAREPPPQNVAVVGEMKPDIEATVKLGLN"
     gene            2015..>2377
                     /locus_tag="VNR65_05345"
     CDS             2015..>2377
                     /locus_tag="VNR65_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWJ18137.1"
                     /translation="MDASEPHNTYHDPALLIEERRGTACILTLNRSSARNSLSLDLIA
                     ALNDAIGRAGAMREAATIIITGVPPAFCSGHDLRELTAHRRDPDRGRAFFEQTMQSCS
                     ALMRSVVACHKPVIAAVNG"
BASE COUNT          499 a          679 c          714 g          485 t
ORIGIN      
        1 gcgaaatacg cctcgtgatc cttatcgttc acggtgatct tgccctgtcc gggcttgacc
       61 cacacgcgcg cgacagcgtt cttccttttg ccggtcgcat atgcgcgccc atgagcgtcg
      121 agcttctgca cgtgaaccgg ccgcgaccgg tcttcggcag caggagcgcc ggcgccctta
      181 tccaagccgg aaaatgtctt tgcgcttgtc tcaggcatta gcgagccctc gctacatttt
      241 tggagttcat ggcaggaaca tcgagttttt ccgggctttg ggccgcgtgc ggatgctcgg
      301 gcccggcgta aatgcgcaaa ttggtcatct gcttgcgtcc aagcggcccg cgcggaacca
      361 tgcgctccac ggctttcata agaacgcgct cagggaactt gccgccgagg atctcgaacg
      421 gacggcgctc cttgatgccg ccaggatggc cggtgtgccg gtagtagatc ttgtcctgcg
      481 tcttgttgcc ggtgaagacg atcttctcgg cgttgatgac gacgatgtgg tcgccgtcgt
      541 ccatatgggg cgtgaatgtg ggcttatgct taccgcgcag gcgattcgcg atgatcgtcg
      601 cgacgcgtcc gacgacaagg ccctcggcgt cgatcaagac ccacttcttc tcgatctctg
      661 atggttttgc ggaataggtt ttcatcgtgt gtccggtttg agcctgcggc tccaatgaaa
      721 aaagggccgc acggcccgtc tgtaaagaga gatagatcag caagccaggc gtgtcaacca
      781 agggatcgag attgcggttg aaaaattgtc ttgcaaaaac aatggcataa actaatggta
      841 ttatagcacc acaaattcgg tatcataata cctcaataat gaggcagtga ataggttgca
      901 gtggcgtgcg ccgcaagctc cgtctcgccc tccgaacaaa gcgcgatttc gcctactgca
      961 agccgcttcc caagcttcaa caggcgcgtt tcgccgagca tgtcgcgctg atccggcttc
     1021 ctgagaaagt tgatcgtgag attggtggtc accgcgtgcg ccacaggtcc gattgacgcc
     1081 agcaccgccg cgtacatcgc aacatcggcg agcgcaaaca tggcgggacc ggatatcgtg
     1141 cctccgggcc tcagcaaatg gtcgccctag accatgcgca tcctcgcaaa gccgtgccgt
     1201 gtttcctcga ctttgtggac gcgcccgatg tcgatctgag ggaagacctc agcgaggaac
     1261 agattgacct ccgccgcgga caatttcggc tgcgctagct tggggacgtc gtgatcgtgc
     1321 aacatccgtt ccgtgatttg accaagattt ggggcaatga gagcggctgc gaaacggtcg
     1381 aatgaccgag cggaaggatt tcccagcatg gccgatttca agaaaagtgc cggacttatt
     1441 ctacttggcc tgatggctgc tgtcttgtcg gcgattggtc cggtagccgc caaggatgtg
     1501 gcgctcaccc aggacaatgt cagccgattc ctggcgtcgt ttgcggaaat gcgcgccatc
     1561 gcgatgatcg agggggtgcg gacaggcatg gatgcggaaa cctcgaagaa ccccgtcggc
     1621 gtcatcgtca aggcgatcaa atcgagtaaa ctgcaagccc aggcgcaagg catcgcggtg
     1681 aagcacgggt tcgcagacat cagggaatgg tcagacaccg gaagagcgat cgggcaggcc
     1741 tatctttacg tgacggcagg tccagctcgc ggcatcgcca gggagacgct caacaaaaac
     1801 aaggacaccg cgatcaagga attggagaag cttggccttc tgaacgataa gcagaaggag
     1861 cggcttcggg agaacctcga tgatctcagc gatcaactcg cgcgcgagcc tcctccgcaa
     1921 aacgtcgccg tcgtcgggga aatgaagccg gatatcgaag ctactgtgaa acttggcctt
     1981 aactgaggta tcctggcatg agggaaaatc gcaaatggac gcctccgagc ctcataacac
     2041 ctatcatgac ccggcgttgc tgatcgagga gcgacgtggc actgcctgca tcctgacgct
     2101 gaacagatcc tcggcgcgaa atagtctctc gcttgatctg atcgccgcgc tgaacgacgc
     2161 gatcggccgc gctggcgcga tgcgagaggc cgccaccatc atcatcacgg gtgttccacc
     2221 cgccttctgc tcgggccatg atcttcgaga attgacggcg caccggcggg atccggaccg
     2281 tggccgcgca ttcttcgaac agacgatgca gagctgttcg gccctgatgc ggtccgtcgt
     2341 tgcgtgccac aagccggtga tcgcggccgt gaacggc
//
LOCUS       DATMGX010000332         2840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_92964, whole genome shotgun sequence.
ACCESSION   DATMGX010000332 DATMGX010000000
VERSION     DATMGX010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92964"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(450..1481)
                     /locus_tag="VNR65_05350"
     CDS             complement(450..1481)
                     /locus_tag="VNR65_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ18138.1"
                     /translation="MEQYVALDVSLQKVAVCVLNASGRVVLEAEVASEPGVLIAFIRT
                     KAPHAVRIGLESGPTSPWLWHSLKSAGLPVVCMDARHAHAALSVRPTKSDRNDARGLA
                     EMVRMGWYKEVQVKSLAAHERMALLGVRRRLVDMRVEIDGQIRGILKTFGLVVGCGNS
                     NAFAQRARILAQDHPVLSALIDKLIEVREVAVAKVAILDKEIRCFVRADPTLRRFMTV
                     PGVGPVTALAFRSTVDEPDRFKRARSVGPYLGLTPRRYQSGETDRQGKISKCGDRFTR
                     TCLYEAANVLLTKVQRWSPLKAWGTRLAKRIGSKKARVAIARKIAVILQCIWSDGTEF
                     WWTKEAVKA"
     gene            complement(1595..2544)
                     /locus_tag="VNR65_05355"
     CDS             complement(join(1595..2205,2205..2544))
                     /locus_tag="VNR65_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489531.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWJ18139.1"
                     /translation="MSKKTLSLDLRERVVAAVSGGISRRQAAERFGVSAASAIRWCAR
                     EKATGSPAAGKRGGDRWSARIDVHKGLILSLVDEVCDITLKELQARLAERGHSFSIGA
                     LWRFFDRHDITLEKKTAHASEQERPDILKQREDWFEGQLDLDPEKLVFIDETWASTNM
                     ARKCGRAPRGQRLRASVPHGHWKTTTFVAGLRLNGLTAPMVLDGPINGVWFQAYVDQV
                     LVPTLSPGDIVIMDNLGSHKGADVRKAIEAVGASLLYLPPYSPDFNPIEKAFSKLKAL
                     LRKAAERTVDGLWNRIGELLEAFTPNECANFLTAAGYEPL"
     gene            complement(2580..2813)
                     /locus_tag="VNR65_05360"
     CDS             complement(2580..2813)
                     /locus_tag="VNR65_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18140.1"
                     /translation="MQEALKDNNYRAWGNIFDVTRDVSAGYASGTLRTGVWWERGDNW
                     RIQEYISLTTRPAFSRAVSCLNESMGIPESGEV"
BASE COUNT          569 a          859 c          800 g          612 t
ORIGIN      
        1 cgctccgcct gggcctccag ctctagcagg tctcgctcca gcagcttttt gagcttttcc
       61 atctctcgca cgatccgggc tcttgtccaa agcatcggct ttctcctcga tcgaatggcg
      121 cctagtggtc gaaatctaac atttggttcc cgcgatttgg agcggcgcat gcggttgcta
      181 ttggaccggt tttgaccgag gccgttctct aatcggcatg tcgaagtcaa gcgctgggga
      241 aactgatgcc ttggccgagg tcacggttct cttcgaggtc gactctgagt cgcctcatca
      301 tgggtgtcag gggcgaaacg ctgcaacgtg tgaagcgccc ttcttccccg aaaggatagt
      361 gggcaagagt ctgctcgcag tcgtttcaac ccgccgtccc agccagggca gcgtctccgc
      421 aggtgcggag ctagggggtg gattagggat caggccttaa cggcctcctt cgtccaccag
      481 aactcggtgc catcggacca tatgcattgc aggatgaccg caatcttacg cgctattgcg
      541 acacgggcct tcttagagcc tatgcgtttc gccaagcgcg ttccccacgc tttgagcggc
      601 gaccaccgtt gcaccttagt aaggagaacg ttggcggctt cgtaaaggca agttcgggta
      661 aatcggtctc cgcactttga gattttgcct tgtcgatccg tttccccgga ttgataccgt
      721 cgcggcgtca atcccagata ggggccgacg cttcgggctc gtttgaatct gtctggttca
      781 tcgactgtag agcgaaacgc cagtgctgta accggaccga cgccgggtac agtcataaag
      841 cgccgcaatg tcggatcggc gcgaacaaag caacgaattt ccttgtctag aattgcgacc
      901 ttggcgacgg caacttctcg cacttctatc aatttgtcga tgagcgctga caacaccggg
      961 tgatcttgcg cgaggatccg ggcacgttgg gcgaacgcat tcgaattgcc acaccccact
     1021 accaagccga acgtctttag gatgccacga atttggccgt cgatctctac ccgcatatct
     1081 acgagacgac ggcgaacacc caacagagcc atcctctcat gagcagcgag actcttgacc
     1141 tgaacttcct tataccagcc catccgcacc atctccgcta acccgcgcgc gtcgttgcgg
     1201 tcggatttgg ttggacggac ggacagagca gcgtgtgcat ggcgcgcgtc catgcacacc
     1261 accggcaatc cagcggactt taaagaatgc cacaaccacg gtgatgtcgg cccgctttcc
     1321 agaccgatcc tgaccgcatg aggtgctttg gttcgaatga atgcgatcaa aactccaggc
     1381 tcggatgcca cctccgcttc gaggacaaca cggcctgacg cattcaaaac acaaacggcg
     1441 accttctgaa gtgagacgtc taatgcaacg tattgctcca tgactgttct cccggggttg
     1501 agcggcaaaa tcagatttgt ttgacatgcg cttcgggcgg aaagatgccg cgccgattac
     1561 cccatgtgtg gaaagtcggg ctagcggttt cccatcaaag cggctcatag ccagcggcag
     1621 ttaagaagtt tgcgcactcg tttggcgtga aggcctccag cagttcgccg atcctattcc
     1681 ataggccatc gacggttcgc tcggcggctt ttcgcagcag ggctttcagt ttcgagaagg
     1741 ccttttcgat cggattgaag tcgggactat agggcggcag atagagcaaa ctggcgccga
     1801 ccgcctcgat cgccttgcgc acgtccgcac ccttgtggct gcccagattg tccatgatca
     1861 cgatgtcgcc tggcgagagc gtcgggacaa ggacttggtc gacataggct tggaaccaga
     1921 cgccgttgat cgggccgtcg aggaccatcg gcgcggtcaa cccgttcagc cgaagcccgg
     1981 cgacgaaggt cgtcgtcttc caatgtccgt gcggcacgct ggcgcgcaac cgctggccgc
     2041 ggggcgcgcg gccgcatttg cgcgccatat ttgtcgaagc ccaggtctcg tcgatgaaga
     2101 cgagcttttc gggatcgaga tcgagttgtc cctcgaacca atcttcgcgc tgcttcagaa
     2161 tgtccggccg ctcttgctcg ctcgcatggg cggtcttttt ttccatgtga tgtcgtgtcg
     2221 gtcgaagaag cgccacagcg cgccgatgct gaagctatgc ccacgctcgg cgagacgcgc
     2281 ttgaagctct ttgagcgtga tgtcgcagac ctcgtcgaca agcgacagga tcaaaccctt
     2341 gtgcacgtcg atacgcgccg accagcggtc cccgccgcgc tttcccgccg ccgggctgcc
     2401 ggtcgctttc tcgcgcgcac accagcgaat ggcgcttgcc gcagaaacgc cgaagcgctc
     2461 cgcagcctgc cgacgggaaa tgccgcccga gaccgcggcg acgacgcgtt cacgaaggtc
     2521 cagcgaaagg gtcttcttcg acatgcatgc cggcctcctt cgccagcatg cagtttgaat
     2581 caaacttcgc ccgattcggg aatccccatc gattcattca gacaagaaac tgctctagag
     2641 aacgccggtc gagtagttag actaatatat tcctgtatgc gccagttgtc gccgcgctcc
     2701 caccatacgc ccgtgcgcaa cgtgccgctg gcatagccgg cgctgacgtc gcgcgtcacg
     2761 tcaaagatat tgccccaggc gcgataatta ttgtccttaa gagcctcttg caaaattatt
     2821 tatgaaatag gcgcgcgaaa
//
LOCUS       DATMGX010000333         2203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_329155, whole genome shotgun sequence.
ACCESSION   DATMGX010000333 DATMGX010000000
VERSION     DATMGX010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329155"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1146)
                     /gene="aroF"
                     /locus_tag="VNR65_05365"
     CDS             complement(130..1146)
                     /gene="aroF"
                     /locus_tag="VNR65_05365"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWJ18141.1"
                     /translation="MIVAMLEHASEDQIQRVTDHLMKMGFAVHRTTGARQSVLAAVGK
                     RIDFDTRDLEVLPGVERVHRISAPYKLAGRQFHEHDTVVKLPNGTEIGGKEVVVMAGP
                     CSVESREQLFAAAEVVSKSGAKVLRGGAFKPRSSPYTFQGLGLEALKLLREAGDRYGM
                     AVISEVMEISQIEIMLPYIDIFQAGARNMQNFNLLRELGKVRKPVLLKRGIAATIEEM
                     LLSAEYIMSGGNYQVILCERGIRTFETYTRNTLDISAIPVVHKLSHLPITSDPSHGTG
                     LRDKVAPMACASVAAGADALLIEVHPSPDEALSDGAQSLYPQQFSELMDQLRTIAPAV
                     GRTI"
     gene            complement(1292..1606)
                     /gene="pheA"
                     /locus_tag="VNR65_05370"
     CDS             complement(1292..1606)
                     /gene="pheA"
                     /locus_tag="VNR65_05370"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HWJ18142.1"
                     /translation="MDIEDWRQKIDAVDRQLVRLINERAQCAQEIGKLKRDSAMPIYE
                     PDREKIIFQNIARINAGPLSDVQLRQVYERLVDVMRQLQREEMAPEAENQGEATELGP
                     ND"
     gene            complement(1643..>2203)
                     /gene="trpA"
                     /locus_tag="VNR65_05375"
     CDS             complement(1643..>2203)
                     /gene="trpA"
                     /locus_tag="VNR65_05375"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWJ18143.1"
                     /translation="EVAKQLRQQSNAGVIIFSYLNPILQFGLEKFCAAAAEAGVDGAL
                     VTDLPVEEAAEYLRAMKAHKLAPVFLAAPTSPEKRLREVAKHSRGFVYAISRTGITGT
                     RQELAGDARSLVERLRLHTKLPIAVGFGVSTPEQFAEVGGFADAAVVGSAIVQRIEQN
                     PGREAAAVSEFLSSLTGTAQEAVGRS"
BASE COUNT          441 a          705 c          582 g          475 t
ORIGIN      
        1 cttcaaccac cccaacaagc gcaaaagcgg cgctcgttgg ggacccctgt tcgcccgctc
       61 gtgatgacaa taataaaaaa cttaacggcg cacctaaagg tgtgcccctt caagacctta
      121 aagctcagct caaatcgtcc ggcccaccgc gggagcgatc gtccgcaact ggtccatcag
      181 ttcggaaaac tgttgcggat acagggactg agcgccatcg gacagcgctt cgtcgggcga
      241 cggatgcacc tcgatcagca aggcatcggc cccggcggca accgaggcgc acgccatagg
      301 cgcgactttg tcacgcaatc cggttccatg cgaaggatcg gaggtgatcg gcaggtgcga
      361 taacttgtgc acgacaggaa tggcggagat atcgagtgta ttgcgcgtgt aggtctcaaa
      421 cgtgcgaatg ccgcgttcgc acagaataac ttggtagttg ccgcccgaca tgatgtactc
      481 ggccgagagc agcatctcct cgatggtcgc cgcgatgcca cgcttcaaca ggaccggttt
      541 tcgcactttg cctaattcgc gcagcaagtt aaagttttgc atgttgcgcg cacccgcctg
      601 gaagatgtct atgtacggca gcatgatttc gatctgcgag atctccatga cctcgctgat
      661 taccgccatg ccgtagcggt cgccagcttc ccgcaacagc ttcagcgcct caagtccgag
      721 cccctgaaag gtatatggcg agctgcgcgg cttgaaggca ccgccccgca ggaccttggc
      781 gccagacttg ctcaccacct ccgccgcggc aaacagttgc tcccgcgact ccaccgaaca
      841 tggaccagcc atcaccacaa cttccttgcc gccgatttcg gtcccgttgg gaagcttaac
      901 gacggtgtcg tgttcatgga actggcgccc cgcgagtttg taaggcgcgc tgatacggtg
      961 cacgcgctct acgcctggca gcacctccag gtcacgcgtg tcaaagtcga tacgcttgcc
     1021 caccgcggca agcacactct ggcgcgcgcc agtagtgcga tgcacggcaa agcccatctt
     1081 catcaggtga tcggttaccc gctggatctg atcttcactg gcatgttcca acatcgcaac
     1141 aatcatgatc tcgctcttcg cctttcgcta ttcgctattc gctattagct attagctttt
     1201 agctcttagc ctttagcaat ccgaactaac ctgtgacggc tcccggacgt tccaaactaa
     1261 ctactagcca ctaaccccta accactgcgc ttcagtcgtt cggccccagc tcagtcgctt
     1321 ctccctgatt ttccgcttcg ggagccatct cttcccgctg aagctgccgc atcacatcca
     1381 ccaggcgctc gtagacctgg cgcaattgca cgtctgacag tggccccgca ttaatgcgcg
     1441 cgatgttctg gaagatgatc ttctcgcggt ccggctcata gatgggcatt gccgagtcgc
     1501 gtttgagctt gccgatttcc tgggcgcatt gagcgcgctc gttgatcagt cgcaccaatt
     1561 ggcggtcgac agcatcgatc ttctggcgcc aatcctcgat gtccattacc tatttgcctc
     1621 gcggtcttgc gacgccgcaa tcttagcttc tgcctacggc ttcctgcgct gttccggtaa
     1681 gggaagatag gaattctgac acagcggccg cttcccgtcc cggattttgt tcgatcctct
     1741 gcacaatcgc gcttcccacc acagctgcgt cggcgaaacc cccgacttcg gcgaattgct
     1801 cgggagtcga aactccaaac ccgacagcga tcggcaattt ggtgtggagt ctcagccgct
     1861 cgaccaacga tcgtgcgtcc cccgcaagtt cttgccgcgt accggtaatg ccggtgcgcg
     1921 agatcgcata cacgaatccg cgcgaatgtt tcgccacctc gcgcaagcgc ttctccgggc
     1981 tggtgggagc tgccagaaac acgggcgcga gtttatgcgc cttcatagcg cgcagatatt
     2041 ccgcggcctc ttccaccgga agatcggtga ccagagcgcc gtccacgccg gcctcggcgg
     2101 cagccgcaca aaatttctcc agtccaaact gcagaatggg attgaggtac gagaagataa
     2161 tcactccggc gttcgactgc tgtcggagtt gcttcgctac ctc
//
LOCUS       DATMGX010000334         2424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_218244, whole genome shotgun sequence.
ACCESSION   DATMGX010000334 DATMGX010000000
VERSION     DATMGX010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218244"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VNR65_05380"
     CDS             complement(<1..464)
                     /locus_tag="VNR65_05380"
                     /inference="COORDINATES: protein motif:HMM:NF015228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HWJ18144.1"
                     /translation="MEAASKAQDELERIEKQIEQLEGAAGDNQEARRQLQEMHVRVDA
                     LRRQIAGQLTPWERTELARHPMRPYPLDYIERIFSDFSEIHGDRGFGDDAAMVCGMAT
                     FRGRQVLVLGTQKGRDTKQRIHRNFGQPNPEGYRKALRAMRFAEKWQHPVIAL"
     gene            complement(659..847)
                     /locus_tag="VNR65_05385"
     CDS             complement(659..847)
                     /locus_tag="VNR65_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18145.1"
                     /translation="MIDELHTLCLERKGAARVLFDLERKGDFTVVMEAEGYNVLPDRT
                     FIHRVEELCGRGSVRVVD"
     gene            complement(905..>2424)
                     /gene="dnaE"
                     /locus_tag="VNR65_05390"
     CDS             complement(905..>2424)
                     /gene="dnaE"
                     /locus_tag="VNR65_05390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWJ18146.1"
                     /translation="IIDDALRLIEQTRGERLDLQKLALDDKETYERVFHTGLTSGVFQ
                     FESHGMRDVLRRYKPTCVEDLTALNSLYRPGPIQGGMIEDFVERKWGRKKVEFDLPEL
                     EPLLKETFGVIVYQEQVMQIANRLAGYSLGEADLLRRAMGKKDPEAMAKQRERFVRGA
                     TERGFPPKKIEKLFDLMEQFAGYGFNKSHSAAYALLAYQTAYLKTRYPVEFMAALLTS
                     VSGSTDDVVKYINECREMGIAVEPPDVNVSDAYFTPHGEAIRFGLAAVKNVGHNAIES
                     IVAARKEAGEFKSIFQFCEKVDLHLLNKRVIESLIKSGAMDRLGRREQLMAVTDSAME
                     HAQKTQKDAALGQHGLFGVFQQDDAPQAEKPLPNIPDWDEHQRLGHEKEILGFFITGH
                     PLEKYREKLLDFNALSTTEVAELKSSTGRDEVLIGGILKNIRVAKSKKGDLYAQGQLE
                     DMNGSVDVLCFADAYKRLADKLKLTVPVLAKGGVRVEDGSNSKLMIGDITPLEQA"
BASE COUNT          442 a          743 c          687 g          552 t
ORIGIN      
        1 agcgcaatga ccggatgttg ccacttctcg gcgaagcgca tcgcgcgtaa tgccttgcga
       61 tagccctcgg gattaggctg gccaaaattg cgatggatgc gctgcttggt gtcgcggcct
      121 ttttgcgtgc cgagcactag cacctgccgt ccgcgaaagg tcgccatgcc gcagaccata
      181 gcggcatcgt cgccaaaacc gcggtcgccg tgaatctcgc tgaaatcgga aaagatgcgc
      241 tcaatatagt ctagcggata aggacgcatg gggtggcgcg ccaactcggt gcgctcccat
      301 ggcgtgagct ggccagctat ctgacgccgc agcgcgtcca cccggacgtg catttcctgc
      361 aactggcggc gggcttcctg gttgtcgccg gcagcgccct ctagttgctc aatctgtttc
      421 tcgatgcgtt ccagttcgtc ctgcgctttc gaagcagctt ccatattggt ttcttgtcgt
      481 ttgtttgacg ctccaccgga attatttacc acggaggcac ggaggacacg gaggtcttgg
      541 tttctgtcga tttgtctccg tgatctccgt gcctccgtgg ttaattttca ttcccatggg
      601 cgggcagtgg gcccattcgg cgcacgggag agagcttcca ccccggagat ccttacactc
      661 aatcaacaac ccgaacgctg ccgcgtccgc atagctcttc cacacggtgg atgaatgttc
      721 ggtctggcag aacattatag ccttccgcct ccatcaccac ggtgaagtcg cctttgcgtt
      781 ccaggtcgaa cagcaccctg gccgcgcctt tgcgctccag acaaagcgtg tgcagttcgt
      841 cgatcattgc tggggagacg ccttcgatag gtatcttgat acgcaaatgc tgcggcagct
      901 taggctaggc ctgctccaac ggagtgatgt cgccaatcat gagcttggag ttggatccgt
      961 cttcgacgcg cactccgcct tttgccagca cggggacggt gagcttcagc ttgtctgcaa
     1021 gccgcttgta ggcatcggcg aagcaaagga cgtccaccga gccgttcatg tcttcgagtt
     1081 gtccctgcgc gtagaggtca cccttcttcg acttggcaac gcggatgttc ttcaggatgc
     1141 ctccaatcaa cacctcatcc ctgcccgtgg aagatttcaa ttccgcaacc tcggtggtgg
     1201 atagggcgtt gaagtcgagc agtttttcac ggtacttctc cagcgggtgg ccggtaatga
     1261 agaagccgag gatttccttc tcatgcccca gccgttggtg ctcatcccag tctggaatgt
     1321 tgggcaacgg cttctcggcc tgcggtgcgt cgtcctgctg gaagacgcca aacagtccat
     1381 gctgtccaag ggccgcatcc ttttgtgtct tctgggcatg ctccatcgcg ctgtcagtaa
     1441 cggccatcaa ctgctcgcgg cgcccgagcc tatccatcgc gccgctcttg atcagcgact
     1501 cgatcactcg cttgttgagc aggtgcagat cgaccttttc gcagaactga aagatcgact
     1561 tgaactcgcc cgcctctttg cgcgccgcca caatggattc gatcgcgttg tggccgacat
     1621 tcttgaccgc cgccaacccg aagcgaatcg cctcgccgtg cggcgtgaag tacgcatcgc
     1681 tgacattcac gtcgggaggc tcgaccgcga tccccatttc gcggcactcg ttgatgtact
     1741 tcaccacgtc gtcggtgctt ccggagacgg aggtgagcag cgcggccatg aactcgaccg
     1801 gatagcgagt cttcagatac gccgtctggt aggcgagcaa cgcgtaagcg gccgaatgcg
     1861 acttgttgaa gccgtatccg gcgaactgct ccatcaagtc gaaaagcttt tcgattttct
     1921 tcggcgggaa gccacgctcg gtagcgcctc ggacaaaccg ctcgcgctgt ttggccatcg
     1981 cctcgggatc tttcttgccc atggcgcgac gcagcaggtc ggcttccccg agcgaatagc
     2041 cggccaggcg attggcgatc tgcatcacct gctcctggta aacgatgacg ccgaaggtct
     2101 ccttcaggag cggttccagc tcgggcaggt cgaattcgac tttcttccgt ccccatttgc
     2161 gctcgacgaa gtcttcaatc atgccgccct ggatcggccc tggacgatac agcgagttca
     2221 acgcggtcag gtcctcaacg caggtcggct tgtaacggcg caacacgtca cgcatgccat
     2281 gcgattcaaa ctggaagacg cccgacgtca acccggtatg gaacaccctc tcgtaagtct
     2341 ctttgtcgtc caacgcgagc ttctgcaggt ccagccgttc gccgcgcgtc tgttcgatga
     2401 ggcgcagcgc gtcgtcaatg atgg
//
LOCUS       DATMGX010000335         2962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_461803, whole genome shotgun sequence.
ACCESSION   DATMGX010000335 DATMGX010000000
VERSION     DATMGX010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461803"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VNR65_05395"
     CDS             complement(<1..335)
                     /locus_tag="VNR65_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18147.1"
                     /translation="MINVTAQSILRKLWPDQREMPRERRALLFLARQSATGVRPALVL
                     AAAFSLFLLCTQGSQAKGSSSCEEVAELSVLASPVAPWRGAPLLVLFAAEKPMEGELS
                     LISPDGRVAA"
     gene            739..2142
                     /locus_tag="VNR65_05400"
     CDS             739..2142
                     /locus_tag="VNR65_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18148.1"
                     /translation="MQPAEAGLSLVIWQGWGVLSVMAALTALLATILAWLLPFPDRSL
                     NRQLSQYNDRMALRDRLGAAPPFKAYLVTVRRFNQWLTEWFGPALSGQAFERCLAIAF
                     IFPIALLLITLVANGISAYRITTWQAVLFAAGFLIIAYVIVVAFRNLLLLIEQGWTTL
                     GGDYAIAQTIARILLGGFAFMVAFTMAFAIASAFSGQISSNAGSVLGAMAGGFALAFV
                     LAVAFALAGATLFSVTIAILAGAALAFASEFTLLLFLFFILLPLTNASIDWLSWSATR
                     FLLRQAEHAEPDILGELKVFGAVLGTFASGALLMIALTALLPNTLELLNFAFTSASLP
                     RFNWEALLNKAIESPWTEGLFVTGMLMTAIVPASVHLIVGLTGIFARFTPGARTAAHS
                     ISDHPEAPLTAKEQMPVKITLILSRIWYLVAAGLTASLIIGASALIYITHAPVARFLG
                     DVALCATAWSHGKCVWF"
     gene            2334..>2962
                     /locus_tag="VNR65_05405"
     CDS             2334..>2962
                     /locus_tag="VNR65_05405"
                     /inference="COORDINATES: protein motif:HMM:NF019005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030701 - NAD+-dinitrogen-reductase
                     ADP-D-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--dinitrogen-reductase
                     ADP-D-ribosyltransferase"
                     /protein_id="HWJ18149.1"
                     /translation="MGANRRRGIGHSTNLLGRPTEWLASCAFNDDPVPLHIAGVREMN
                     RSLFDMLAQAGDLAEAGEAFTCYMMAMFGIDPEQREGAGLGTGRRRYRSSFPRLIEGW
                     GFDSNGPEGAVLKGWVESRFGIGPSFHKEVIERVSTRAWTTYVEEKMSSRFHNNAIYV
                     QLDLLFEFCQWAIQRFAYPGQTHLTLFRGINALDEHWIVERIDKRNAVI"
BASE COUNT          583 a          872 c          848 g          659 t
ORIGIN      
        1 gccgccacgc gtccatcggg cgagatcagc gaaagctcgc cctccatcgg cttttcagcg
       61 gcaaacagga cgagcaaagg cgctcctctc cacggcgcga cgggggaggc cagcaccgac
      121 agttcggcca cctcttcaca ggacgacgag cctttagcct gcgagccctg agtgcataaa
      181 aggaagagag aaaatgcagc cgcgaggacc aaggcgggcc tcactccggt agcagactga
      241 cgcgccagaa acaaaagcgc gcggcgctca cgaggcattt cgcgctggtc cggccaaagt
      301 tttcgcaaaa tcgattgtgc ggtcacgtta atcattcgac ctcaataaac gaccacggca
      361 ccgatccgtc cctggcggac agagcggcgg ctattgttac gcactagagc agccagcatc
      421 catatgaaca cggataagct gttctagcgt tttagaatag atcaaacttt ccgcccttaa
      481 tcgatctgat tagcagcggg ttgatccatg catcctgcgc gcctttcttt gtggaatctt
      541 gtgaccgatt tgcgtcctga tcgtcgtcaa gatgtccgaa atcgacagtt ttctaccgaa
      601 agtcccaaaa accctgtgaa gattcatatt acgggacaga aaagcgcccc ttagctgcta
      661 ccacgctcat gcgcagcttg catgggccgg tttaggttgc taaccaagcg gcggcggggc
      721 ggattttttg gggggcgtat gcaaccggct gaagcgggcc tgtccttggt gatatggcag
      781 ggatggggcg tcttatcggt catggcggcc ttaaccgcgc tgctggcgac aatactggct
      841 tggctcctgc catttcccga ccgctcgctc aacagacagc tttcgcagta caacgaccgc
      901 atggcgctga gggatcgcct tggagccgcg ccgcctttca aagcctatct cgtcaccgtg
      961 cgccgcttca accaatggct cacggaatgg tttggcccgg cgcttagcgg ccaggctttc
     1021 gaacgatgcc tcgccatcgc gttcatcttt cccatcgcgc tgctccttat aacgctcgtc
     1081 gccaacggta tcagcgcata ccgaatcacg acctggcaag ccgtgctttt cgccgccggg
     1141 ttcctgatta tcgcttacgt catcgttgtc gcattcagga atttgcttct gctgatcgag
     1201 cagggctgga cgacgctcgg cggagactat gcgattgcgc agacgattgc ccggattctt
     1261 ctcggcggat tcgccttcat ggtggccttc acgatggcat tcgccattgc ttcggcattc
     1321 tcaggccaga tatcgtcgaa tgcaggctcg gtgcttggcg ccatggcggg cggattcgcg
     1381 ctcgcatttg tgctggctgt tgcatttgca ctcgcaggag cgacgctttt ctctgttacg
     1441 atcgctatcc ttgccggagc cgcactcgcc ttcgcgagcg agttcacgct gctcctcttt
     1501 ctattcttta tcctgctccc cctcacaaac gcgtccatcg attggctatc ctggagcgcg
     1561 acacgctttc tgctgaggca ggcggagcat gccgagcccg atatcctcgg agagctgaaa
     1621 gtcttcggcg cagtactcgg cactttcgcc tccggcgctt tgcttatgat tgcgctgacg
     1681 gcgcttctgc cgaacacgct agaactcctg aacttcgcct tcacctcggc cagccttccc
     1741 aggttcaatt gggaagcgct cttgaataag gcgatcgagt ctccctggac cgaggggttg
     1801 ttcgtaacgg ggatgctgat gaccgcgatt gtgccagctt cggtgcatct tatcgtcggc
     1861 ttgacaggga tttttgctcg cttcacgccg ggcgcgcgaa ccgccgcaca cagcatttcg
     1921 gatcatcctg aagcgccgct tacggccaaa gagcagatgc cggtgaagat cacgctgatc
     1981 ctgtcgcgca tttggtacct cgtggccgca ggattgaccg caagcctcat cataggcgca
     2041 agcgcactga tttacatcac ccacgcgccg gtcgcccgat tccttggcga tgttgcctta
     2101 tgcgcgacgg cgtggagcca cgggaagtgc gtctggtttt gatgcgcctc ggctcagcca
     2161 agatagaaag ctgagattga gacccaccgg cgcttcccca ttcccatcca gcaacagttg
     2221 ggctacctcg ctggaagcgt ttttgccggg tcggtaatat tcgacccgcc gcaggaattg
     2281 gtgcaacact aagcgccgtt gaaggagggc gcttcttcag gcaaacaaaa gtcatgggag
     2341 caaacaggcg gcgcggcatc ggacattcca ccaatcttct ggggagacct accgagtggc
     2401 ttgctagctg cgcattcaac gacgatccgg ttccgctcca cattgcgggc gtgcgcgaaa
     2461 tgaaccggag cctgttcgac atgctggctc aagctggcga tctggccgag gcgggcgaag
     2521 cgttcacatg ctatatgatg gccatgtttg gtatcgatcc cgaacagcgc gaaggagcag
     2581 gacttggcac cggacggagg cgctatcgct cgtcctttcc gcgattgatc gagggctggg
     2641 gtttcgacag caacgggccg gaaggcgcgg tactcaaggg ctgggtcgaa agccggtttg
     2701 gcataggccc ttccttccat aaggaggtca tcgagcgcgt ttcgacccgc gcctggacca
     2761 cctatgttga ggagaagatg tccagccgct tccacaacaa cgccatttat gtgcagctgg
     2821 acctgctttt cgagttttgc caatgggcga ttcagcgttt tgcatatcct gggcaaacgc
     2881 atttgacgct gttccgcggg atcaacgcgc tcgatgagca ttggattgtg gagcggatcg
     2941 acaagcgcaa tgcagttata ca
//
LOCUS       DATMGX010000336         2112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_304625, whole genome shotgun sequence.
ACCESSION   DATMGX010000336 DATMGX010000000
VERSION     DATMGX010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304625"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1744)
                     /locus_tag="VNR65_05410"
     CDS             complement(230..1744)
                     /locus_tag="VNR65_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWJ18150.1"
                     /translation="MKRNFITAVLWLLAAAALLDALGPAQAADKKPNVVFILADNVGY
                     GDLGVYGGGELRGAPTPRIDQLAREGLRLTQFLVEPACTPSRAALMTGQYSIRNGLSL
                     VLVPGSEATLSAHAYTMGDMFHGLGYATAIYGKWHLGSEPQSLPTAHGFDEFYGIPPD
                     ISWDSATYVDTIELTHSIDAPPDVLLSKGPQIVESVLGGPLRRVKPFTRKVRAEIDTE
                     LTTKSIAFMKRSHAAGKPFFLYLPFSMGHFPNLPSKDFAGKSQIGQYGDKLMEGDFHV
                     GQILDALKELRLDDDTILMFASDNGPQGETAREMGNDATQDMGNSGPFRGELGEATEG
                     AIRTFAFICWPGHVKPATTSYAMFSIMDFFPTLASIVGGKMPTDRPIDGVDQSDVLFG
                     KSLSGHRDHLLTFIGPQLVAERWKQWRVYFTDIHPTGIGPERLPGMASASGTMAGYPK
                     VYNIQMDPHEDHNVAAMFGWVADPALREVRKYEESVKKYPNPLAPNITVFGRGG"
BASE COUNT          378 a          641 c          649 g          444 t
ORIGIN      
        1 tgatgtcttg atgtcttgat gtcttgatgt cttgtggcct gtggcctgtg gcgcgtccag
       61 actttccatc ctctcaagat tcccgcggtc tttttgctta actgggcgcg gtgtatcaga
      121 tgagaacctc ggtgattgtt ctgatcgctt cggcaaacag ccctagaccg ttaggcggcg
      181 ttttcgagcg aagtacgtca gccaaagcgc agcttgcgct taacgccgtt tagccgcccc
      241 ggccaaacac ggtgatgttg ggcgccagcg ggtttggata tttcttgacc gactcctcgt
      301 atttcctgac ttcccgtagc gcgggatcgg cgacccagcc gaacatcgcc gcgacattgt
      361 ggtcctcatg cgggtccatc tggatgttgt agaccttggg gtagccggcc atcgttccgc
      421 tagcggaagc catccccggc agacgctccg ggccgatgcc ggtcgggtgg atgtcggtga
      481 aatagacgcg ccattgcttc catcgctccg ccaccagttg cggtccgatg aaggtcaaga
      541 ggtgatcgcg atggcctgat aggctcttgc cgaacagaac gtcgctctgg tccacgccat
      601 cgatcggccg atcggtcggc atcttgccgc cgacgatgct ggccagggtc ggaaagaaat
      661 ccatgatcga gaacatggcg tacgacgtcg tggccggctt gacgtggccg ggccagcaga
      721 tgaaggcgaa ggtgcggatc gcgccctcgg tggcctcgcc gagctcgccc cggaacggac
      781 cggaattgcc catgtcctgc gtcgcgtcgt tgcccatctc gcgcgcggtt tcgccttgtg
      841 gcccgttgtc cgaggcgaac atcaggatcg tgtcgtcgtc aagcctgagt tccttcagcg
      901 cgtctaaaat ctggccgaca tggaagtcgc cttccatcag tttgtcgccg tattggccaa
      961 tctgcgactt gcccgcaaaa tccttcgacg ggagattggg gaagtgcccc atcgaaaatg
     1021 gcaggtagag gaagaacggc ttgcccgccg cgtggctgcg cttcatgaag gcgatggatt
     1081 tcgtcgtcag ttccgtatcg atttcggcgc ggactttccg cgtgaagggt ttgaccctgc
     1141 gcaacgggcc gcccagcacg gactcgacga tttgcggccc ctttgacagc agcacatcgg
     1201 gcggcgcgtc aatcgagtgc gtgagttcaa tcgtgtcgac ataggtcgcc gaatcccagg
     1261 agatgtcggg cgggatgccg tagaactcgt caaaaccgtg cgcggtcggc aggctctgcg
     1321 gctcgctccc gaggtgccac ttgccataaa tcgccgtggc gtagccgagc ccgtggaaca
     1381 tgtcgcccat cgtgtaggcg tgcgccgaga gcgtggcctc ggagcccgga acgaggacca
     1441 gcgataggcc gttgcggatg gaatattgcc ccgtcatcag cgctgcacgc gatggcgtgc
     1501 aagcgggttc gaccagaaac tgggtgagcc gtaaaccctc gcgcgccagc tggtcgatcc
     1561 gcggtgtcgg cgcgccgcgt agctcgccac ccccgtagac gcccaagtcg ccatagccga
     1621 cgttgtcggc gagaatgaac acgacattgg gttttttgtc cgctgcctga gctggaccga
     1681 gtgcgtcaag aagcgcagcc gcagccagca gccacaagac tgcggtgatg aagttgcgtt
     1741 tcatgctaac tccttgctag ttcctcaaag ctcgcgacga aagctttccg cgccctcgct
     1801 caagaaggta ggagcgcggc gcgcctatct tccaacaatg cgcatcgatt ctcccaccct
     1861 cagactattc aataccttgc cgtgccggct ccttattcaa gcagagtttg acctcactct
     1921 cccccgccgg gagcatgacg ggacgccaac ctagcttggc gtccccggtc gaactcagtc
     1981 cggtgccccg cccgtcgggc tcgtaagctt cgtcatcaca ttattcaggt tgaacgagcc
     2041 gggcttttgg cgcggcggga aatcctgaaa ggtttgcagc cattgggcga cgtagcccgc
     2101 cgccggtgcg at
//
LOCUS       DATMGX010000337         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_197659, whole genome shotgun sequence.
ACCESSION   DATMGX010000337 DATMGX010000000
VERSION     DATMGX010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197659"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="VNR65_05415"
     CDS             complement(<1..1399)
                     /locus_tag="VNR65_05415"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWJ18151.1"
                     /translation="MDSEHDYYSILDVPRDASDADIRRAFRSLAKEHHPDSRGAGNGG
                     GSVERDFRLISEAYETLKDSDRREAYDRELDEARQLEPHAANWGKRSFAAGLGVGILI
                     AIVAVGALNYLDRTGRMSGEKAQDSLKIVIGSEKADAGRRVPLDRNKVREAEVTPALP
                     SEAGKAPPPPSPAPATSAAPSPIDVSARLPAERQAPAVSGEAGGRSNDKSVAVAPVEP
                     LEIATGTAGHKKTVRILPGKGLTQSFTDCHSCPEMVVIPAGEALIGSRVEGDGSHWEE
                     APAHRVHLGRSLAISKTVISAGNWRACVDAGVCRLSLSSLLAVGPRVAATRISWSDAK
                     LYVGWLSQTTGWRYRLLTEAEWEYAARAGKGRGPMEPESGGRYTADTMKDTSGLFPRV
                     RFDHFTAAGPNAWGMQAGGVLEWVEDCWHGSYDQAPDDASPWLSAAGGDCANRVVRGN
                     VTAGGGFGWRLSARAQ"
     gene            complement(1895..2503)
                     /locus_tag="VNR65_05420"
     CDS             complement(1895..2503)
                     /locus_tag="VNR65_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18152.1"
                     /translation="MVAFKRVTGAIRVVVLGLSVSVSLPGAAMADTEADNAKLALCAK
                     DICAIIVSKKASGPDLNCDLAKTWEKDQIQKGADSKNLAWGLGSAKCTAKVKAKRSEL
                     IAAVTAPEITFKLDRQSVACEIGTEKYEFRATMAPELTFKQGATTAVSLHMDNIQGAP
                     LIKGVVWTAATLERNFGILQSDMVREVNRFIKKECPKILGRG"
     gene            2686..2829
                     /locus_tag="VNR65_05425"
     CDS             2686..2829
                     /locus_tag="VNR65_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HWJ18153.1"
                     /translation="MDKDQANVNESMDMPAHEKTYKNFTGFVIFGIVTVVIILAGMAI
                     FLT"
     gene            2852..>3441
                     /locus_tag="VNR65_05430"
     CDS             2852..>3441
                     /locus_tag="VNR65_05430"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18154.1"
                     /translation="MITISVTREGEGETRVALSPETTKKFKNLGARVIFQAGAGDRSY
                     MPDQLYKDAGAEIVQNAADAISQADVILKVGRPSQEELQAMKPGAVFAGMLDPFGSRD
                     ELEALAAKGVSAFTMEFMPRITRAQSMDVLSSQSNLAGYKAVVDAAARFGRALPMMMT
                     AAGTVPAARIFVMGAGVAGLQAIATARRMGAVITAT"
BASE COUNT          715 a         1038 c          959 g          729 t
ORIGIN      
        1 cctgagccct cgcggaaagc cgccagccga atcctccacc cgcggtcacg ttgccacgca
       61 caacacgatt ggcacaatcg ccgccggccg ccgatagcca tggcgaagca tcgtcggggg
      121 cttgatcata gcttccgtgc cagcaatctt cgacccattc aaggacaccc ccggcctgca
      181 tcccccatgc gttgggcccc gcggcggtga agtggtcaaa gcgaacgcgg ggaaacagtc
      241 cgctcgtatc cttcatggtg tcggcagtgt agcggccgcc cgactcgggc tccatcggcc
      301 cacgcccttt gcccgccctt gcagcatatt cccactcggc ttccgtcagg aggcgatagc
      361 gccaccccgt cgtttgcgac agccatccga catagagttt cgcgtcggac caggatatcc
      421 gggtggccgc cacacgggga ccgactgcca gcagcgaaga caggctcagg cggcaaacgc
      481 cagcgtccac gcacgcgcgc caattgccgg ccgaaatgac agtcttcgaa atcgcaagtg
      541 acctgccaag atgcacgcga tgcgccgggg cttcttccca atgggaccca tcgccttcca
      601 cgcgcgatcc tataagcgcc tctccagcgg ggatcaccac catttcaggg caagagtggc
      661 agtcggtgaa gctttgggtg agtcccttgc ccggcagaat ccgaacggtt ttcttgtgcc
      721 cggccgttcc tgtggcgatc tcaagaggct cgaccggagc gacggctacg ctcttgtcat
      781 tggatctccc gccagcctcc ccggaaacgg cgggagcttg acgctccgcc ggcagccgcg
      841 cagagacgtc gattggcgaa ggcgctgctg aggtagccgg cgccggtgaa ggaggcggag
      901 gagcttttcc agcttcggaa ggcaatgctg gagttacttc ggcttcccgc actttgtttc
      961 gatcaagcgg cacgcggcga ccggcatcgg ccttttcgga gccaatgacg attttcagcg
     1021 agtcctgagc tttctcgccg ctcatgcggc cagtgcgatc gaggtaattc aaagccccaa
     1081 ccgcgacgat tgctatgagg atgccaacgc caagacctgc cgcaaaagat cgctttcccc
     1141 aattcgcagc atggggctcc aactgccggg cttcatccag ttcccggtca taggcttcgc
     1201 gccggtctga atcctttaga gtttcatacg cctcgctgat cagccggaaa tcgcgctcaa
     1261 cagatccgcc accgttgcca gccccccggc tgtccggatg atgctccttg gcaagggagc
     1321 gaaaggcgcg gcggatatcg gcgtccgagg catcccgggg cacgtccaat atggagtaat
     1381 agtcgtgctc gctgtccatg gcatgcgcct agccgcctat attaacgtta aggcgaccgt
     1441 cggccagccg gcgcgccaat gcgaaactag attgccatct agggcaggga tagggcgtat
     1501 ttggggcggc aatgaagcaa atccgacaca cagactctgt gaaatcgctt tccggttaac
     1561 agcggactac tttgcgtttc cgcgcatcgc tttccttaat cggtctcaga cactcaagct
     1621 tgacagccaa gaggtatccg tatcgcatca tataatgacg cagataagat cctccgaatc
     1681 cgccagcaat tgaggctaca aagcagctcc ccattctatt ctattgaatg tgggatacaa
     1741 gtttcgccgc gcgacctgat tctccgcgac ctttcgcaga ttggggtcga tcatcgatag
     1801 ctcgccgcgc gcgatattat catggcgata attttattgc ttagccctaa gaatgcaagc
     1861 cgcctatgcc atgcgcttag ctaagtctga gacactatcc tctgccgagg atcttagggc
     1921 actccttttt gatgaagcgg ttcacttcgc gaaccatgtc actttgaaga ataccgaaat
     1981 ttctttccag ggtcgcggcg gtccagacga cgcccttgat taacggcgcg ccctgaatgt
     2041 tgtccatgtg cagcgagacg gctgtcgtcg ctccctgctt gaacgtcagc tctggcgcca
     2101 tcgtggcccg gaactcgtat ttttcggttc cgatctcaca agcgaccgat tgcctgtcga
     2161 gtttgaaggt aatctccggc gcggttacgg cagcgatcag ttctgagcgt ttcgctttga
     2221 ctttcgcggt gcactttgcc gaaccgaggc cccacgccaa atttttcgaa tctgcacctt
     2281 tttggatttg gtctttttcc caggttttgg ccagatcgca gttaagatca ggcccactcg
     2341 cctttttaga aacgatgatg gcgcagatgt ccttcgcgca tagggccagc ttggcattgt
     2401 ccgcctcagt gtcagccatt gccgcaccgg gcaaagacac actcactgag agcccaagga
     2461 ctactacgcg gattgcccct gttacccgct taaacgctac catatgccac acttcctctt
     2521 cgcgtgttcc ctcaatgccg gaccagagtt gcactctgta gtactaaaga atgcgcgctc
     2581 ttgtagcact aagagattgg actcaacact tggcgctggc agaaacttgt gtaaatgttt
     2641 gtgcaacaaa aatgggcaaa agcccaccca gggaagggac tggctatgga taaggaccaa
     2701 gccaatgtga acgaatccat ggacatgcca gctcatgaaa aaacctacaa gaacttcacc
     2761 ggcttcgtga tattcggcat agtaacagtc gtgattatcc tggccggaat ggcgattttc
     2821 ctcacttaag cttcttactt ccgtaggctc aatgatcaca atctccgtca cgagggaggg
     2881 ggagggcgag actcgggtag ccctatcccc ggaaacaaca aagaaattca agaatctcgg
     2941 cgctcgcgtc atttttcagg cgggggccgg agaccggtcg tacatgccgg accaactcta
     3001 taaagacgct ggggcggaga tcgtgcagaa cgcggccgac gccatctcgc aagcggatgt
     3061 catcctcaag gttggtcggc cttcacagga agagctccaa gcgatgaagc ccggtgccgt
     3121 ttttgcgggc atgctcgatc cgttcggatc gcgggatgag ttggaagcgc tggcggcgaa
     3181 aggcgtttcg gcctttacca tggagttcat gccgcgcatc acgcgcgcgc agtccatgga
     3241 cgtgctgtcc tcacaatcga acctcgccgg atataaagct gtcgtcgatg cagcggcgcg
     3301 ctttggccgc gcgctcccaa tgatgatgac ggcggccgga acggttcccg ccgcgcggat
     3361 atttgtcatg ggtgctggcg tcgcagggtt gcaggccatc gcaaccgccc gccggatggg
     3421 cgctgtcatt accgcaacgg a
//
LOCUS       DATMGX010000338         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_339169, whole genome shotgun sequence.
ACCESSION   DATMGX010000338 DATMGX010000000
VERSION     DATMGX010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339169"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VNR65_05435"
     CDS             <1..361
                     /locus_tag="VNR65_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18155.1"
                     /translation="TSIRENCLSCHAAPATAVHSGATAQCTQCHSQDRWKPATFDHTQ
                     FFVLDRDHNAACTTCHTTSDRRQYTCYGCHEHTEANIRTKHIREGIPNFTNCVSCHRS
                     AHGESREGSTREREDDD"
     gene            1376..1654
                     /locus_tag="VNR65_05440"
                     /pseudo
     CDS             1376..1654
                     /locus_tag="VNR65_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612341.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1640..2026
                     /locus_tag="VNR65_05445"
     CDS             1640..2026
                     /locus_tag="VNR65_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HWJ18156.1"
                     /translation="MSSTTDTATLLALLVYLVMSLNVARARGKYGVKAPAVTGNEYFE
                     RAYRVQMNTLEQMVFFLPSMWLYALLLTDKGAAFGGLIWVIGRVIYSVSYVRNPASRG
                     VGFTISFIAAIGLFFGASYGVLKAFL"
     gene            complement(2139..>2696)
                     /locus_tag="VNR65_05450"
     CDS             complement(2139..>2696)
                     /locus_tag="VNR65_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18157.1"
                     /translation="NGIHLADAGQHLVPLGRIQGSSAAFAVRVAGSERHRARAFGAEA
                     DAGEQGRPADDSSRRDLRIARSQMRLHGIECGLIDQGWHLDGGAALLWAIELYLTAVS
                     ADFVSATAIDAPGRFCSDPLRAPLNERRSIAVGEAMRAIQYNHCKTIKPKNTINAKVK
                     TVRLQANLKLTHWYEKASLSISAAK"
BASE COUNT          586 a          737 c          748 g          625 t
ORIGIN      
        1 cacgtcgatc cgggagaatt gcctctcctg tcatgccgct ccagcgacgg ctgttcatag
       61 cggcgcgaca gcccaatgca cgcagtgtca tagccaggat cgctggaaac cagccacatt
      121 cgatcacaca caattcttcg tgctcgaccg agatcataac gccgcttgca ccacctgcca
      181 cacgaccagc gatcggcggc aatacacctg ctacggctgc catgagcata ccgaagccaa
      241 tatccgcacc aagcatatcc gagaaggcat accgaacttc acaaactgtg tctcatgtca
      301 ccggagcgct catggcgaaa gcagggaagg cagtacgcgc gagcgtgagg acgatgactg
      361 atctgcccgc ctctcagttc aatccaatcg aaagagccat tcaaacccgt ttcactgaga
      421 gggttacctg acccagcgca ttggttggct tcgcgtagcc gtacttggcg cgaatgatgg
      481 cattatttcg atggcgagct tttcttcggt gtagctttgt tgctaagcat gagggcgctc
      541 ctacaatggt tggggtcgga ctgctcgatc gcagccggcc gccgtggagc aatttctcgc
      601 ttggggtcga cagacggtcg tatttcttct gatactagtt gctagtcctc tgaatcagcg
      661 actcgtaagt tcgcaaattt gaccatcaag ggcagaacca gagcgggcaa accattacgc
      721 agtcgcgtgt tctccagtct cctggccggg taccatatcg tcgccagcca gccggtgcat
      781 ttctgtaacg tgcacccggc gaaaaacggt ggctgtaacc gccgcgattg gcggccggcc
      841 cggcacgacg atgcccgggg ttcattgcag gtcttcccgc gacgatttcc tcgctccatt
      901 cctggcctgt tgcggttgtc cccctgcact cggatttact ccgttttttt ggtgacaagc
      961 ggaaaatgcg cggcaggaat cgccgaagct ggtcccatcg ccaataagaa aaccaacgca
     1021 gccgatagta acgcgctggc taatttcaga ttgtctccgt ggtttgagtt gagacgctct
     1081 tcgaaaggcg gtccgcatag catccttgaa cttatggttc gaccactaag ccatcggctg
     1141 ttggttgctg accgaattgc tgttgaaagg cgcagacctt ggaacttcgt tcaaatcaat
     1201 cgatttgagc gacaagcttg ttgtcgtctg agaggacgcg acgaatggcc gagctttgct
     1261 ttgcaatgat gcgccgtttt gagagagccg aaaagtcaga ttctttccaa aatcaaccag
     1321 aatcgtgaca ctgtgcggtc ttgaccacaa ctgcactgat ccgcatgtcg cgctgtcggt
     1381 gcaggcgaac atgaagaagg atttgcgcga aatttacctg gcgccgagtc gcgctttggc
     1441 cgaggtcgcg atcgatgtct tcgccgagaa ataccaggcg aagcaccaca aggcggtcga
     1501 atgcctgacg aaggatcgcc aagctctgct ggccttcttc gacctccctg ccgagcattg
     1561 ggaccacttg aggacgacaa atcccatcga aagcgtgttc gcgacggtcc ggcacaggac
     1621 ggtgcggaca aaaggttcat tgtcgtcaac aaccgatacg gcgaccctgc ttgcgctcct
     1681 cgtttacctc gttatgtcgt tgaacgtagc tagagcacgg ggaaaatatg gcgtgaaagc
     1741 gcctgctgtc acaggcaacg aatattttga gcgtgcctac cgtgtacaga tgaacacctt
     1801 agagcagatg gtattcttcc ttccgtcaat gtggctctac gcgctactgt tgacggacaa
     1861 aggagcggct tttggcggac ttatttgggt tattggacgg gtgatttatt cggtttccta
     1921 cgtacgaaat ccagcgtcac gtggggtagg atttacgatt tcatttattg ctgcaattgg
     1981 actgtttttc ggtgcttcat acggagttct gaaggcattt ttgtagtcca acgatcaacg
     2041 atggaaggta gagacgagtt cgcagcctcc gcacatttct gctaggagtc tctgctgcaa
     2101 gcgcgtcgaa ccgctcgatg gcttttgtag gtgcaggact acttggctgc actgatcgat
     2161 agcgatgctt tctcgtacca gtgggtcagc ttcaagttcg cttgaagcct gacggtcttg
     2221 accttggcgt ttattgtatt tttgggttta atggtcttgc aatgattgta ttgaatagcc
     2281 cgcatagctt ccccgacagc gatagatcgg cgctcattta gcggcgctct gagcggatca
     2341 gagcaaaagc gccctggggc gtcaatcgct gttgctgaaa cgaagtcggc agagactgcg
     2401 gtcaaataca actcaatcgc ccatagcaag gcagctccgc cgtcgaggtg ccacccctgg
     2461 tcgataaggc cacattcgat gccgtgcagg cgcatctgcg atcgcgcaat ccgaaggtca
     2521 cgccggctcg aatcgtcagc gggccgaccc tgctcaccgg catctgcttc tgcgccaaat
     2581 gcgcgggcgc gatgacgctc cgaaccggca acgcggactg cgaatgctgc tgagctacct
     2641 tggatgcgcc cgagcgggac aagatgctgg ccagcatcgg cgagatggat gccatt
//
LOCUS       DATMGX010000339         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_73821, whole genome shotgun sequence.
ACCESSION   DATMGX010000339 DATMGX010000000
VERSION     DATMGX010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73821"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2318
                     /locus_tag="VNR65_05455"
     CDS             <1..2318
                     /locus_tag="VNR65_05455"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWJ18158.1"
                     /translation="AFSPLASAQEILPFPPTPSASTAGLTIEDSVYRKRVEPKHLADG
                     APNILIILMDDLGPGTASTYGGEINTPTLDRVAKMGISYNRFHSTAMCSPTRAALLTG
                     RNATFVGNGQIAALANDFDGFTGIIPKSSATVAEVLKDYGYNTGAWGKWHNTPEEQIT
                     SKGPFDYWPTGYGFEYFYGFLAGEASQYEPTLVRNTTQVTEEKRKGYHLTDDIAEDAI
                     KWLREQKAYAPDKPFFMYWAPGASHGPHQIMKEWADKYKGKFDDGWDAYRERVFKRAK
                     EKGWIPQETQLTPRPESMASWASIPEDEKPFQRRLMEVFAGFTEHADYNAGRVIDEIQ
                     REGKLDNTLIFYIWGDNGSSSEGLNGTISEQLAQNGIPTKISQHIDALKELGGLDALG
                     GSKTDNMYHAGWAWAGSTPYRGTKLNGAYFGGTRQPMAVSWPKGIKPDATPRAQYHHV
                     IDVVPTIYEITKITPPRVVNGFEQDPIEGVSMRYTLEDAKAAGRRKTQFFDIMASRGI
                     YHDGWFAAAPGPREPWVGGMPKGIKEWSPLTDKWELYNIDQDWSEANDLSAANPKKLD
                     EMKSLFLLESAKNKNLPIGGGLWSTAIFHPEDAPATSLTEWTFDSPMTRMPESAAPKL
                     GKVDSLVSMDVDMPANANGVLYALAGFSGGVTCFIKDGILSYEFNLFEVQRTKIKAKD
                     KLSAGKAKIEVESKLVGPIGGPMNVTLKVNGQVVAQGQVPAAMSLHFTSNATFDIGSD
                     LDSPVSLDYFDKAPFVFNGTIGTTKIVYLKK"
BASE COUNT          585 a          762 c          763 g          468 t
ORIGIN      
        1 ttgcgttctc gccattggca agcgcgcagg aaatcctgcc gttcccgccc acgccgtccg
       61 catccacggc tggcctgacg atcgaggact ccgtttacag aaaacgcgtc gagccgaagc
      121 acctcgccga cggcgcgccg aatatcctga tcatcctcat ggacgatctc ggtccgggta
      181 cggcttcgac ctatggcggc gagatcaata cgccgacgct cgatcgcgtg gcgaagatgg
      241 gcatctccta caacaggttt cactcgaccg ccatgtgctc accgacgcgt gccgcgctac
      301 tcacggggcg caatgcgacg ttcgtcggca acggccagat cgccgcgctg gccaacgatt
      361 ttgacggctt caccggtatc atcccaaaat cctcggcgac ggtcgccgaa gtgctgaagg
      421 attacggcta caacaccgga gcatggggga agtggcacaa cacgccggag gagcagatca
      481 cctccaaggg gccgttcgac tattggccga ccggctatgg tttcgagtat ttctacgggt
      541 tcctcgccgg cgaggcctcg caatatgagc cgaccctggt gcgcaacacc acgcaggtga
      601 ctgaagagaa gcggaagggt tatcatctca cggacgacat cgcggaagac gcgatcaaat
      661 ggctccgcga acagaaagcc tacgcgccgg acaagccgtt cttcatgtac tgggcccccg
      721 gtgcttcgca cggcccgcat cagatcatga aggaatgggc cgacaagtac aaaggcaagt
      781 ttgatgacgg ctgggatgct taccgggagc gcgtcttcaa gcgcgccaag gagaagggct
      841 ggattccgca ggaaacccag ttgacgccac gaccggagtc gatggcctca tgggcctcga
      901 tccctgagga tgaaaagccg ttccagcgcc gcctcatgga agtcttcgca ggcttcactg
      961 agcatgccga ttacaacgcc ggccgggtca tcgatgaaat acagcgagag ggcaaactcg
     1021 acaacacgct gatcttctac atctggggcg acaatggctc ctcgtcggag ggcctcaacg
     1081 ggactatcag cgagcagttg gcgcaaaatg gaattccgac caagatctcg cagcatatcg
     1141 acgcattgaa ggaactcggc ggcctcgatg ctctcggcgg ctcgaagacc gacaacatgt
     1201 accacgccgg gtgggcttgg gcgggcagca cgccataccg gggcaccaaa ctcaatggcg
     1261 cctatttcgg cggcacacgc caaccgatgg ccgtctcgtg gccgaagggc atcaagcccg
     1321 acgccacgcc gcgcgcgcaa taccatcacg tgatcgacgt cgtgccgacg atctatgaga
     1381 ttaccaagat caccccacca cgcgtcgtca acggtttcga gcaggatccg atcgaaggcg
     1441 tgagcatgcg ctatacgctt gaagacgcaa aagccgcggg acgacgaaag acccagttct
     1501 tcgacatcat ggcgagccgc ggaatttacc acgacggctg gttcgctgcc gcgccaggtc
     1561 cgcgtgagcc gtgggtgggc gggatgccga aaggcatcaa ggaatggtcc cccctgacgg
     1621 acaagtggga actctacaac atcgaccagg attggagcga ggcgaacgac ctttctgctg
     1681 cgaatccgaa gaaactggac gagatgaagt ctctcttcct gcttgaatcc gcgaagaaca
     1741 agaatcttcc aatcggcggc ggcttgtggt ccacggcgat cttccatccg gaggatgccc
     1801 ccgcaacgtc tctcaccgaa tggacctttg atagcccgat gacccggatg ccggaatccg
     1861 ccgcccccaa gctcggcaaa gtagatagcc ttgtgagcat ggacgtcgac atgcccgcga
     1921 acgccaacgg cgtgctctac gcgcttgccg ggttctccgg aggcgtgacg tgcttcatca
     1981 aggacggcat cctgagttac gagttcaacc tgttcgaagt ccaacgcacg aagattaagg
     2041 ccaaggacaa gctctcggct ggcaaggcca aaatcgaagt ggaatcgaag ctggtcggtc
     2101 ccatcggagg tccgatgaac gtcaccctga aagtgaacgg ccaggtggtg gcgcaaggcc
     2161 aagtgccggc agccatgtcg ctccacttca catcgaacgc gaccttcgat atcggcagtg
     2221 atctcgattc gccggtatcg ctcgactact tcgataaggc gccgtttgtg ttcaacggca
     2281 ccatcggcac gacgaagatc gtctatctga agaaatagca gcgtatgtcc cgaacaatac
     2341 gctagtgagg tcgttcaaac cttcgcgcag atgtcgggac tagtgtggat aaacgatggc
     2401 ttctggaaaa tctgggggat agcgacatga atacaaagaa atatgcagcc gaagcgatcg
     2461 gtacgttttg gctgacgttt gccggttgcg gcagcgcggt tatcgccgca gcgtttccac
     2521 aggttggtat cggtttggcc ggtgtgtcac tggccttcgg tctaagcgtc gtcacgat
//
LOCUS       DATMGX010000340         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_220544, whole genome shotgun sequence.
ACCESSION   DATMGX010000340 DATMGX010000000
VERSION     DATMGX010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220544"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..>2158
                     /gene="mgtA"
                     /locus_tag="VNR65_05460"
     CDS             193..>2158
                     /gene="mgtA"
                     /locus_tag="VNR65_05460"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HWJ18159.1"
                     /translation="MTVSQSDTTSLYDRMASLLGRKVSRPDARKAGIAISNEIVALSH
                     LDREAVLRQLGSSKDGLDASDAAARLRTMGANSITQEEQPTIFRELLGRAKNPLNGLL
                     LSLAFVSYLLGDMRAAIVIGVMVVLAIGTAFVQEHRSNNTAAKLRAMVKHTATVRRRG
                     LTGDAEKSGFEGFTEIPSESLVSGDIVHLSAGDMIPGDLLLLDAKDLFVNEATLTGEA
                     LPAEKHADPPQSQTADPFECPNLCFMGASVVSGYATGVIVHTGARTYFGQLAESISGQ
                     RELTSFDKGINRFTWLMVRFILVMGPAVFLINGITKGNWLEALLFSVAVAVGLTPEML
                     PMIVTVNLAKGAMSMAKKRVIVKRLHAIQNFGAMNVLCTDKTGTLTQDRIILKRHLDI
                     RGNECERVLEFAYLNSHYQSGLKNLLDTAVLAHVELGQNLILKHRYEKIDEMPFDFVR
                     RRLSIVLKKEDGSHILICKGAVEEIFAVSRHYDLDGEHGDLDPSHYAQAQQEMRALNE
                     DGFRVVAVAYKEFPQPKAVYSVEDESELTLLGYIAFLDPPKDSASTAIAALHASGVAV
                     KILTGDNEIITAKICREVGLDAGRIILGSEIEAMDDQALAKIANETTVFAKLSPPQKA
                     RVIAALHAGGHVVGYLGDGINDTPALKAADV"
BASE COUNT          456 a          627 c          642 g          433 t
ORIGIN      
        1 tggctcaccg aagctctggt cgaacaagga cgcgatgtca ctctatttgc cagttgtggc
       61 cgcgcgagca agtcgcttct tcatggcaaa agggttagtg agcccgcgtg attaatcgcc
      121 ccggtccggg cgccggggtc atagccgcgg attccgcagg aagcgcttac taacgctcat
      181 tcagaggcac ccatgaccgt atcccagtcg gacacgacaa gtctctatga ccgtatggcg
      241 tctctgctgg ggcgcaaggt ttcgcgcccg gacgcgagaa aagccggcat cgccatctcg
      301 aacgagattg tggcgctcag tcatcttgat cgtgaggcgg tcctccggca gctgggatcg
      361 tcgaaagatg gcctcgatgc cagcgacgca gcggcgcggc tgcgcacgat gggcgccaat
      421 tcgatcaccc aggaagaaca acccaccatt tttcgcgagt tgttgggccg cgcgaagaac
      481 ccgctcaatg gtctgctctt aagcctcgct ttcgtctcct atctgctggg cgatatgcgc
      541 gccgccatcg ttatcggcgt catggttgtg cttgcgattg gaaccgcgtt tgtccaggag
      601 catcgctcga acaatacggc cgcaaagctc cgggccatgg tcaagcacac cgctaccgtg
      661 aggcggcggg gcttgactgg agacgcggaa aaaagcggct ttgagggctt caccgaaatt
      721 ccaagcgaaa gccttgtctc cggcgacatt gtgcacctgt ccgcgggtga catgataccc
      781 ggcgacttgc tgctgttgga cgcaaaagat ctgttcgtga atgaggcgac gctcacaggg
      841 gaggcgctgc ccgctgaaaa gcacgccgat cctccccaaa gccaaacagc ggacccgttc
      901 gagtgcccca acctgtgctt catgggggcc agtgtggtga gcggatacgc gacgggcgtt
      961 atcgtccata ccggtgccag aacttatttc gggcagcttg ccgagtccat ctcaggtcag
     1021 cgcgagctta cgagcttcga caagggcatc aaccgtttca cctggctgat ggtccgcttc
     1081 attctggtga tgggtccggc ggtttttctc atcaacggca tcaccaaggg caactggctc
     1141 gaagctctcc tgttctcggt agcggtggcg gtgggattga cgcccgaaat gctgccgatg
     1201 atcgtgaccg tcaatcttgc caagggcgca atgtcgatgg cgaagaagcg ggtcatcgtc
     1261 aaacggctgc atgcgatcca gaatttcggg gcgatgaacg tgctctgcac cgacaaaacc
     1321 ggaacgctca cccaggaccg catcatcctg aaacggcacc tcgacattcg cggcaatgag
     1381 tgcgagcggg ttttggagtt tgcctatctc aacagccact atcagtccgg actgaagaat
     1441 ctgctggata cggcggtgct cgcgcatgtt gaactcgggc aaaatctgat cctgaaacac
     1501 cggtacgaga aaatcgacga aatgccgttc gatttcgtgc ggcggcggtt gtccatcgtt
     1561 ttgaagaagg aggacggatc gcacattctc atctgcaagg gcgcagtcga agagatcttt
     1621 gccgtaagcc gccattatga cctcgacgga gagcatggtg acctggaccc ttcgcattac
     1681 gctcaagctc agcaagagat gcgggctctg aacgaggacg gcttcagggt cgtggctgtc
     1741 gcctacaaag agtttccgca accaaaggct gtctattccg tcgaagacga aagcgagctg
     1801 acacttctag gctacatcgc ctttctcgat ccgccgaagg acagcgcatc caccgccatt
     1861 gcagcgctcc acgcctccgg tgtggcggtg aagatcctca ccggcgacaa cgaaattata
     1921 acggccaaaa tctgccggga agtcggcctt gatgccggcc gcatcatact cggctccgag
     1981 atcgaggcga tggacgatca ggcgctcgcg aaaattgcga atgagacgac ggttttcgcg
     2041 aagctttcgc cgcctcaaaa ggcacgcgtt atcgcggcgc tccatgctgg cggccatgtt
     2101 gtgggctatc tcggcgacgg catcaacgac accccggcgc tcaaggccgc cgacgttg
//
LOCUS       DATMGX010000341         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_342287, whole genome shotgun sequence.
ACCESSION   DATMGX010000341 DATMGX010000000
VERSION     DATMGX010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342287"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..479)
                     /locus_tag="VNR65_05465"
     CDS             complement(216..479)
                     /locus_tag="VNR65_05465"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWJ18160.1"
                     /translation="MEGAKRERPLDSGSMAPTLPRVFELRAEDRSLWYRVLYVKLEGA
                     VYVLHCFTKKTNKTPPRDIEIARGRLSALKLRLAARKKNRLSR"
     gene            complement(701..1132)
                     /locus_tag="VNR65_05470"
     CDS             complement(701..1132)
                     /locus_tag="VNR65_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18161.1"
                     /translation="MLSRNPEAAKRFAPDKVIELESEYNRLKGRFSNKKGEVRHRWTT
                     KSIGKMAGEMGRTDQYELVYSLGSSMHHRLTGYVEMKGDSVTMDGPPSLAWVGSSLIA
                     AHTYLLFALDTLNQGCKLAFDEKLTAAGDEFQGLWRGRACG"
     gene            1663..>2481
                     /locus_tag="VNR65_05475"
     CDS             1663..>2481
                     /locus_tag="VNR65_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWJ18162.1"
                     /translation="MAVYAVQRFWFMRAWSWIGSFSNSNWRFGLHAVLIALAAMLLAT
                     VLDPLLGHGVSRLSSGSFNLGKSLTTITRLWMVASFFGFLAVESVGAIEWVTNVAARL
                     LPHSGAIAGGFSPSRRTFFQYAAVLAGGFPFFAATYGFAAGRLRYTIERVDVPVANLP
                     AELDGLRIAQLSDIHIGDYMPPLEIARAVTMANDLQPDISFVTGDFVSSEGDPLDACI
                     TELSRLRAPLGVWGCNGNHEIYAGVEDEAERLFREKGMRLLRASNSVVEHNGGRF"
BASE COUNT          515 a          698 c          658 g          610 t
ORIGIN      
        1 cgatctcaga tcgctaatcc tcggctgcga tgtttgcagg atgttctgta gttcgcgctg
       61 cgaataacgc gacgcaactt tcacgatctt ggtgtgcagg tcggccttga gcttcaacgc
      121 tgcggctttc tccggtgaga atccaatgtc ctcgaacaga ttgggtctca ttgcgtttct
      181 cctattgtgg gtcagtgacc ctgatttgca gcagcttacc tagacaacct gttctttttt
      241 cgtgccgcga gtctcagctt caaggcggac aatcgccctc gcgcaatctc gatgtctctc
      301 gggggagtct tgttagtctt ctttgtgaag cagtggagaa cgtaaaccgc cccctcaagc
      361 ttcacgtaca gtactcgata ccacaagctc ctgtcttcgg cgcgtaactc aaacaccctt
      421 ggcagggtag gagccattga gccggaatcc aagggcctct cccgttttgc accctccata
      481 ggtcggctcc aaggtcctct cggatatcgt cagggaagga tctgatgacc gccctcgagt
      541 ccccttcgaa aaacacatcc ggaggtgagg catcagccac ggtgagaacg tatcagagtt
      601 agggagaatt gtatataaga acttatatga cgtgcgaggc tttgattatc aagggcttgc
      661 cagcctgcgg gtgggattcc cccgatcgcg tcgtcatcaa tcagccgcac gcccggcctc
      721 tccacaggcc ctgaaactca tcgcccgcag cagtgagttt ctcatcaaag gcgagtttgc
      781 aaccttgatt cagggtgtct agcgcaaaca acaaatatgt gtgcgcagct atgagcgacg
      841 aaccaaccca cgccaatgag ggagggccgt ccatcgtcac actgtccccc ttcatttcga
      901 cgtagccggt aagtctatga tgcattgagg accctaaact gtaaaccaat tcatattggt
      961 cagtgcgccc catttccccg gccatcttgc caattgactt cgttgtccat cgatggcgaa
     1021 cctcgccctt tttgtttgag aatctacctt ttaaacggtt gtactcgctt tcaagctcga
     1081 tcactttatc cggcgcgaat cgtttggccg cttcgggatt ccgcgacaac atccattaat
     1141 acctacgcca agagagcact ctcccaaaat caatgtagtc gtccacctct accgggttca
     1201 gtctcaagta ttccgccaaa gttgaagtct caaacatccc gcgaacaatc ttcatggcac
     1261 cggggcctga accattccca cacagcaaca gagcatcgtt aaaaccgaaa gcggtcaacc
     1321 gggttagaaa atgtatggca ttctccacag gcattgcaac tctctcatcg ccgctgacaa
     1381 gaatctggcc cgtaagttgc gcaaactgag tgaaagagca gaagacaggg gcatatagct
     1441 cgtgaacctg cggccaaaaa tccggatatc caaaactgat cgtctcgtca gatatgcaca
     1501 tactttcaat accagcagca gatagcgaac acaaggcgaa caacaattgc tggtggtgcg
     1561 cagccgagtc ctccgccgcg cagaacgcat ctaacttgga gaaatgttgc ttggagtatt
     1621 gccctgattc ttacgcgagt tcttctcttc gcgctgtttc ttttggcggt ctacgctgtc
     1681 cagcgcttct ggttcatgcg ggcgtggagc tggattggct cgttctcgaa ttccaactgg
     1741 cggtttgggc ttcatgccgt actgatcgcg ctggcggcta tgctcctggc tacggtgctc
     1801 gatccgctgc ttgggcatgg cgtctcgcgg cttagctcgg gcagcttcaa tctcggtaaa
     1861 tcgctcacca cgatcacccg cctgtggatg gtcgcttcat tctttggatt cctggcggtc
     1921 gagtcggtgg gagcgattga gtgggtcact aacgtcgcgg cccggcttct gcctcattct
     1981 ggagcaatcg cgggtgggtt cagtccgtcg cgccgaactt tctttcaata tgccgccgtg
     2041 cttgctggag gctttccatt ttttgccgct acttatggat ttgccgccgg gcgtttgcgc
     2101 tacaccatcg agcgcgtcga tgtgccggtt gccaatcttc ctgcggagct tgacgggctg
     2161 cgcatcgccc agctcagcga catccacatt ggcgactaca tgccgccgct tgaaattgcg
     2221 cgcgcggtta ccatggcgaa cgatctgcag ccggacattt cttttgttac gggagacttt
     2281 gtctcgagcg aaggcgatcc gctggacgcc tgcattacgg agttaagccg gctgcgcgct
     2341 ccgctgggcg tttggggatg caatggcaac cacgaaattt atgcgggggt ggaagatgag
     2401 gcggagcggc ttttccgcga aaagggaatg cggctgctgc gcgcctcgaa ttcagtggtt
     2461 gagcataacg gggggcgctt c
//
LOCUS       DATMGX010000342         2913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_372743, whole genome shotgun sequence.
ACCESSION   DATMGX010000342 DATMGX010000000
VERSION     DATMGX010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372743"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VNR65_05480"
     CDS             <1..1455
                     /locus_tag="VNR65_05480"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18163.1"
                     /translation="KPDMIHPLIEPVLRETFGVIIYQEQVMQLAQVLSGYSLGEADLL
                     RRAMGKKIKAEMDAQGAHFVKGAMEKGIAEARARYIFELVAKFAGYGFNKSHAAAYAL
                     IAYQTAYLKANHCQEFLAASMTLDMGNTDKLYAFTREAKQHGIAILPPSVNKSAVDFE
                     PEGGDIRYALAALKNMGQAAAAYIVEERGDKPFRDLSDFMSRVNPKTVNKRAIETLNA
                     AGALEDLEKNRAKVAKNVERLIEYGARLAHDRAAGQEDMFGTASAETAAHPLILSDAP
                     AWGLLETLDAELAAAGFYLSGHPLDDFVDQLARLNVMRWSDFAARTQAHGRAEARLAA
                     TVTYKQDRKAKSGNRFAFAGFSDPTGQFEAVIFADTLASAGDKLEPGTNVLLQVEAEA
                     DGEAIKVRVQTVQTIDEVFGKGTRQMSITATEKLQVGDLIKHLSRDGSLELKLVVELR
                     EASKAVEILLGNNFSISARQLSALKTLPGVVHIG"
     gene            complement(1545..1706)
                     /locus_tag="VNR65_05485"
     CDS             complement(1545..1706)
                     /locus_tag="VNR65_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18164.1"
                     /translation="MSRFIQNASDAFASSRSPTRQFANVIKLNAVDYGLLAMLLVTLV
                     SIGATLVGN"
     gene            1917..2795
                     /locus_tag="VNR65_05490"
     CDS             1917..2795
                     /locus_tag="VNR65_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWJ18165.1"
                     /translation="MQKLLVVGAAVMDCVYTLDELPVAAEKFRAKSLALRGGGTAATA
                     AVTARRLGIDVALAARIGDDFFGDAIVDELQNESVDCKLVRKCPGCRSSVSAVMIDSR
                     GERMLVNFKDSNIDSGTDWLPQKLPSEIHGVLGDHHWEAGTEHLFRLAREAGKPAILD
                     ADRQTSMSILASATHIGFSARGLREQSGIESLPEALAWCQTQVPGWLAVTDGENGALF
                     TEEGGIAHEPALKVLAVDTNGAGDVWHGALAVALLEGQEPRKAVRFANAAASIKCSRD
                     GGRSAIPTQSEVAAFL"
BASE COUNT          629 a          821 c          882 g          581 t
ORIGIN      
        1 aagccggaca tgatccatcc gttgatcgag ccggtgctgc gcgagacctt cggggtcatc
       61 atctatcagg agcaggtgat gcagcttgcg caggtgctgt cgggctacag cctcggcgaa
      121 gccgatctgc tgcgccgcgc catgggcaag aagatcaagg ccgagatgga cgcccaaggc
      181 gcgcactttg ttaaaggtgc aatggagaag ggcatcgcgg aggctcgcgc gcgctacatc
      241 ttcgaacttg tggcaaaatt cgcgggctac ggcttcaaca agagccacgc cgcggcctac
      301 gcgctgatcg cctatcagac tgcgtatctc aaggcgaacc attgccagga atttctcgcc
      361 gcctccatga cgctcgacat gggtaacacc gacaagttgt acgccttcac gcgcgaggcg
      421 aagcagcacg gcatcgcgat cctgccgcct tcggtgaaca agtcagcggt cgatttcgag
      481 ccggagggcg gcgatatccg ctatgcgctt gccgcgctga aaaacatggg acaggcggcg
      541 gccgcgtaca tcgttgagga gcgcggggat aagccgtttc gcgatctttc cgatttcatg
      601 tccagggtca atccgaaaac tgtaaacaag cgcgcgatcg aaacgctgaa cgcggcgggc
      661 gcgcttgagg acttggagaa gaacagggcc aaggtcgcga aaaacgtcga gcgccttatc
      721 gagtacggcg cgcgtcttgc ccatgaccga gctgcggggc aggaagacat gttcggaacg
      781 gcctcagccg aaactgcggc ccatccgctg atcctttcgg atgcgccggc ctggggttta
      841 cttgaaacgt tggatgcgga gcttgcggcg gcaggcttct atctttcggg tcatccgctc
      901 gacgactttg tggatcagct tgcgcgcctc aacgtgatgc gctggagcga tttcgcggcc
      961 cgcacccaag ctcacggacg cgccgaagct cgccttgccg cgaccgtaac ctacaagcaa
     1021 gaccgaaagg ccaaatccgg aaaccgcttc gccttcgcgg gcttttccga tcccacagga
     1081 caattcgagg ccgtgatttt cgccgacacg ctggcgtccg ccggcgacaa gcttgagccc
     1141 ggcacgaacg tgctgttaca ggtcgaggcc gaagcggatg gcgaagccat caaagttcgt
     1201 gtccagactg tacaaacgat cgacgaagtt ttcggcaaag gcacgcggca gatgtcgatc
     1261 accgcgaccg agaaacttca ggttggcgat ctcataaagc atctgagccg cgatggctcg
     1321 ctcgaactca agctcgtggt tgaactgcgc gaggcgagca aggccgtgga aatcctcctc
     1381 ggaaacaact tctcaatatc ggcccggcag ctttccgcgc tgaaaacgct gcccggcgtt
     1441 gttcacatcg gctgagcagg attgtatggt ttcgccaacg aatgggccaa ttttaccttt
     1501 ggccgcttca gcctcggaag cggcagctga cgcgattacc cgcgttaatt cccgacgagc
     1561 gtagcgccaa tcgaaaccag ggtgacgagc agcatcgcca ggaggccata atcgaccgca
     1621 ttgagtttga tcacattggc gaattgccgg gttggggacc tgctcgaagc gaaggcgtca
     1681 ctcgcatttt gtataaagcg actcatggca tactcacttg gctcggttgt tatgtaactc
     1741 ttaccacccc cgaatttcat gatcgccaag tcacagcctt gtttttgcgc cttattgtat
     1801 gatgatttag ccgtctcacg aatacggttg ttcaaacccc agggcatcag ccttgaggca
     1861 aaatgtgctt agatattagc gagatcgaag acgcaaacaa aaacgtcggg cgttgtatgc
     1921 aaaaactcct cgtggtcggc gcggcagtca tggactgcgt ttacaccctg gatgagcttc
     1981 cggtagcagc agagaaattc agggcgaaaa gcctcgctct gcgcggaggc ggaacggcgg
     2041 caaccgctgc tgtaaccgcg aggcggctag ggatcgacgt ggctttggcc gcgcgtatcg
     2101 gcgatgattt cttcggcgac gccattgtcg atgaactgca aaatgagagc gtcgattgca
     2161 agctggttcg aaagtgcccc ggctgccggt cctccgtctc cgccgtcatg atcgatagtc
     2221 gcggagaaag aatgctcgtc aacttcaagg attccaacat cgatagcggc acggactggc
     2281 ttccacaaaa gctgccgtct gaaatccatg gcgtgttggg cgatcatcat tgggaggctg
     2341 gaacagagca cttgtttcgt ctcgcccgcg aggctggaaa gcctgcgatc ctcgatgcgg
     2401 atcgccagac ctcgatgagc attttagcca gcgcgaccca tatcgggttc tcggcgcgtg
     2461 gtctccggga acagtccggc atagaaagcc tgccagaagc gctggcttgg tgccaaaccc
     2521 aagtgccggg ctggttggcg gttaccgacg gggaaaacgg tgctttgttt actgaagagg
     2581 gcggtatcgc gcatgagcct gcattaaaag ttctcgcggt cgacacgaat ggagcgggcg
     2641 acgtctggca cggagcgctt gctgttgctc tcttggaagg gcaagagccg aggaaggcgg
     2701 tgcgttttgc aaatgccgcc gcttccataa aatgttcgcg ggacggcggg cgctccgcaa
     2761 tccccaccca aagcgaagtg gcggcattcc tgtagagtga gactggcttt acggcttatt
     2821 gtcatggccg gacctgaccc agccatccac gttcgattaa caaggattgg cgaccgggat
     2881 ggccgcatca agtgcggcca tgacgcccgt ctt
//
LOCUS       DATMGX010000343         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_41793, whole genome shotgun sequence.
ACCESSION   DATMGX010000343 DATMGX010000000
VERSION     DATMGX010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41793"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VNR65_05495"
     CDS             <1..928
                     /locus_tag="VNR65_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18166.1"
                     /translation="HVVPIPLDRNPFSTNTTVAFPASASLTPYTDPYFRGFDNQFPDY
                     YRYTEWARDFDANYAKGKLPSLSLVRFMHDHTGDFAAAIDGVNTPELQQADNDYAVGL
                     LVQKIANSIYANNTLIFVVEDDSQDAGDHVDSHRSVAYVAGAYVKQKALVSAQYNTIN
                     LVRTIEEVLGLPPMNLNDALARPMADIFNTQPSRWSFTATPSAFLYGTDLPLPPKPAS
                     LIVPKPTHNAQYWARVTKGMDFTSEDRMDFGDYNRILWKGLMGREPYPDAPTGMDLRQ
                     NREVLLARYQRSLKQKAAQARLGGKSADTSHP"
     gene            1483..2106
                     /locus_tag="VNR65_05500"
     CDS             1483..2106
                     /locus_tag="VNR65_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ18167.1"
                     /translation="MDAKRNNAKGPQPREGKATQMEGPDLESVIERAQGHDAEALGEI
                     YRLYVRRVFGLCRYMLDSRESAEDATSEVFLKLQRSIESYDGSIPFPRWLLRVAGNQC
                     IDALRRRKRGRQVIVEVEEGVAVIEAASSEPSPLGAVLSTEERAQVRDAIARLPENYR
                     VPLVLRYYGELSYDEIAQQLGLQRNYVAALIFRAKQELRRKLGHRSK"
BASE COUNT          442 a          699 c          623 g          424 t
ORIGIN      
        1 ccacgtcgtc cccattcccc ttgaccgaaa cccgttctcc accaacacga cggtggcctt
       61 tccggcgagc gcttccctca ccccatacac cgatccgtac tttcgcggtt tcgacaacca
      121 gtttccggat tattaccgtt acacggaatg ggcgcgagat ttcgatgcca attacgccaa
      181 agggaaactt ccttcgttga gcctggttcg cttcatgcac gatcacaccg gcgattttgc
      241 tgccgcgatt gatggcgtga atactccgga actgcagcaa gccgataacg actatgcggt
      301 cggcctgctc gtgcagaaga tcgcaaacag catatacgcg aacaacacgc tgatctttgt
      361 agtggaagac gactcgcagg atgccggcga ccacgtcgat tcgcaccgca gcgtcgcgta
      421 cgtggcgggc gcctacgtga agcaaaaagc gctggtctcc gcgcagtaca acaccatcaa
      481 cttggtgcgc acgattgaag aggtcctggg ccttccgccg atgaatctga acgacgcgct
      541 cgcgaggccc atggccgaca tcttcaacac ccagccgagc cggtggagct ttaccgccac
      601 tccttcggcg tttctctacg gtacggacct gccgctgccg cccaagccgg ccagtctgat
      661 cgtgcccaag ccgacgcaca acgcgcagta ctgggcgcgg gtcacgaagg gaatggactt
      721 cacttccgaa gatcgtatgg atttcgggga ctacaaccgc atcctctgga aagggttgat
      781 gggcagagag ccttatcccg acgcgccgac cggaatggat ctgcgccaga accgcgaggt
      841 gctccttgcc cgctaccagc ggtctctgaa gcaaaaagcg gcgcaggcac ggctgggcgg
      901 caagagcgcc gatacatcgc atccctagtc aactccgttc accccctgga cggctcacaa
      961 ctgccgcatg aaaccgctgt ccaccacacg aaaacttcgc gtgctccctc acgctccgcc
     1021 cccgtgcgcc ttgccgccca acccatctct tcttcgcgct tctccacact ctgcgttagc
     1081 caccgttccg cgcttctgcc agatcccggg cgcgccgggt tgtcttctgc gccacgccgc
     1141 agccctccac cccacctcga catctcacct tcctcccatt gaatatgcat acacccgcgt
     1201 ccgccgcccg agcggcttcc ttctgacggc tttatcggac gcgcgcccca acgctctgtt
     1261 tctctcgact cttcttcttg tgagagcgcg cctccgataa agcactaacg tcatccgtcc
     1321 ccaattgggc gcggcaagca ccatccgatc atccgatttc ccaaattggg cctcgtggct
     1381 ttggggaaga tcgcattctc cgagtgggta ggcgcgtcca gcccagggtt cctcatcccc
     1441 taaaatctgt gtaactttgt tcgatctgca gcgtactgct ttatggacgc caagagaaac
     1501 aatgcgaaag gaccgcagcc ccgcgagggc aaagccacgc aaatggaggg gccggatctg
     1561 gagagcgtaa tagaacgcgc acaaggccac gatgccgagg cgctggggga gatctatcgc
     1621 ctctatgtcc ggcgcgtatt cggcttgtgc cgttacatgc tggactcacg ggagagcgcg
     1681 gaagatgcca ccagcgaagt cttcctcaag ctgcagcgct ccatcgagag ctacgacggg
     1741 tcaataccgt tccccaggtg gctgctgcgg gtggctggca accaatgcat tgacgctttg
     1801 cggcgccgga agcgtggacg gcaggtgatt gtggaagttg aagaaggagt cgcggtcatc
     1861 gaggcagcca gctcggaacc gtcgccgctg ggtgccgtcc tcagcacgga agagagggcg
     1921 caggtgcggg atgccattgc ccgtctgccg gagaactatc gcgtgccgct ggtcctgcgc
     1981 tattacggcg aattgagcta tgacgagatc gcacagcagt tgggcctgca gaggaactac
     2041 gtggcggcgt tgatatttcg cgccaagcag gagctgcgcc ggaaactggg ccacaggagc
     2101 aagtgatcat ggaatgttat tcggaacaga tcgtctctat cttcgtggac ggtgaactgg
     2161 cggtggagga ggcgcggcgc ctgcgaga
//
LOCUS       DATMGX010000344         2108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_153833, whole genome shotgun sequence.
ACCESSION   DATMGX010000344 DATMGX010000000
VERSION     DATMGX010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153833"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VNR65_05505"
     CDS             <1..533
                     /locus_tag="VNR65_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWJ18168.1"
                     /translation="VMIKRFLDAGAQTLLLPMVETAQEARRAVAATRYPPQGIRGFAG
                     AARASRFGRVKDYHVRAHEELCVLVQVETKLGLQNLEAIACVEGIDGVFIGPGDLSAA
                     LGYLGQQKHPDMVGTIEKAIRLIRELGNAPGILTSDEALARRYIDAGCLFTAVGVDIS
                     ILARGAEALAARFKPS"
     gene            complement(530..820)
                     /locus_tag="VNR65_05510"
     CDS             complement(530..820)
                     /locus_tag="VNR65_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWJ18169.1"
                     /translation="MEIGLPHVVVWIIVGILAGSVTGMIVKGDRRGFGVFRNLGLGMA
                     GALLGGLLFRIFRLFPNLDKFSLSVRDVISALIGSALVLLLLWVWQRLPKRG"
     gene            1060..1518
                     /locus_tag="VNR65_05515"
     CDS             1060..1518
                     /locus_tag="VNR65_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18170.1"
                     /translation="MTAQPSSPFDISPMLKMYTKSFELWKKNYENMMKNAKDTQGDFG
                     ANGAAQVTHSPAGSTSTHDAALSNWRKSSEHLFKRLVQSRIELCNFFGSRWEQYLKLP
                     EQLSQCRSLTEFGNLQSSFLSQFAKDYMHETEKLAHPVAELMTNWSSAKK"
     gene            1644..>2108
                     /locus_tag="VNR65_05520"
     CDS             1644..>2108
                     /locus_tag="VNR65_05520"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-hydroxybutyrate polymerase N-terminal
                     domain-containing protein"
                     /protein_id="HWJ18171.1"
                     /translation="MIGSANAIDRFYHYLLTKQSGGVSPAGLWLSYLDWAVHLSLAPG
                     TQAKLAWRAMSDAIALQHYVASVLENGDGKAHCVDPAPSDKRFANPGWERWPFNALQQ
                     SFLLCEGWWNEATSNIRGVSAHHAEPVNFAARQLLDMVSPSNFLPTNPELLER"
BASE COUNT          497 a          639 c          575 g          397 t
ORIGIN      
        1 cggtcatgat caagcgcttc ctggatgcgg gcgcgcaaac tttgctcctg cccatggtcg
       61 agaccgcgca ggaggcgaga cgggctgtgg ccgcgacgcg ctatccgccg caaggcatcc
      121 gcggctttgc aggcgctgca cgtgcctccc gcttcggccg agtgaaggac taccatgttc
      181 gcgcgcatga agaactgtgc gtgctggtcc aggtcgaaac caagctcggc cttcaaaatt
      241 tggaagccat cgcttgcgtg gagggcatcg acggcgtctt catcggcccc ggcgatctat
      301 cagcggctct cggctatctc ggtcagcaga agcacccgga catggtgggg acaatcgaga
      361 aggcgatccg tctcatccgc gaactcggga acgcgcccgg catccttacg agcgatgaag
      421 cgctcgcgcg acgctacatc gatgccggat gccttttcac cgccgtggga gtcgacattt
      481 ccattctcgc acgcggcgcc gaagcgcttg cggcgaggtt caagccgtct tagccgcgct
      541 tgggcagtcg ctgccacacc catagtaaca acagcaccag cgccgaaccg atcagtgccg
      601 aaatcacatc gcgcacggac agcgagaact tgtcgaggtt aggaaagagc cggaaaattc
      661 ggaacaacag gccacccagg agcgcgcccg ccatgccgag gccgagattg cggaaaacgc
      721 caaatccccg gcggtccccc ttgacaatca tgccggttac cgagccggcc aggatcccga
      781 caatgatcca aacgacgaca tgcggcaggc caatttccat ggcgtttctt ccctgctgaa
      841 gccaaagatc attgagttcg aaaagccatc ctaagcttag ctataagaaa actctccctt
      901 aaacaagctc taccatgaag ctggcctttc agattacccc tcttgtcagc ttcacaggca
      961 cggaaattta ctaatccagt cttgataacc atcaacttaa taaaactgac atcgagtgat
     1021 gctttaaagt gcggagaacc tggtccggag tagtacgcca tgacagcaca accatcatcg
     1081 ccgttcgaca tctctccgat gctgaaaatg tatacgaagt ccttcgagct ttggaagaaa
     1141 aactacgaga acatgatgaa gaatgcaaag gacacgcaag gcgatttcgg ggcaaacgga
     1201 gccgcgcagg tcacgcattc acctgccggc agcacttcca cccatgatgc tgctttgtcg
     1261 aactggcgaa aatcgagcga acaccttttc aagcgcctcg tccagagccg gatcgagctc
     1321 tgcaactttt tcggcagccg ctgggagcaa tatctcaagc tacccgaaca gctctcccaa
     1381 tgccgctccc tgaccgagtt cggtaatctg cagagctcgt ttctaagcca gtttgcgaaa
     1441 gactatatgc acgagacgga gaaactcgcg cacccggtgg ccgagctgat gacgaactgg
     1501 agcagcgcaa aaaagtaaac acatagcggt atgatagtcg agcaatgcag cacgcgcgac
     1561 acagcgttgc ggcgcagccc attgcgcttg aattccaaac ctaagctgcg caactattga
     1621 caaacagcat atgggaggga gcaatgatcg gaagcgcaaa cgccatcgat cgtttctatc
     1681 attatctctt gacgaaacag tcgggcggag tctctcctgc cgggttatgg ctgtcctacc
     1741 tggattgggc cgtccacctt tccctcgctc cgggaacgca ggcaaagctc gcgtggaggg
     1801 caatgagcga tgccatcgcc cttcagcact atgtggcgtc tgtcctcgag aacggggacg
     1861 gcaaggccca ttgcgtcgac ccggccccaa gcgacaagcg gttcgctaac cccgggtggg
     1921 agcgctggcc cttcaacgcg ctccagcaaa gcttcctcct ctgcgagggg tggtggaacg
     1981 aagcgaccag caacatcagg ggcgtgagcg ctcatcatgc cgagcccgtc aactttgcgg
     2041 cgcgtcagct tctcgacatg gtttcaccgt ccaatttcct gccgacaaac cccgagctcc
     2101 ttgagcgg
//
LOCUS       DATMGX010000345         2814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_359773, whole genome shotgun sequence.
ACCESSION   DATMGX010000345 DATMGX010000000
VERSION     DATMGX010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359773"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..622
                     /locus_tag="VNR65_05525"
     CDS             200..622
                     /locus_tag="VNR65_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ18172.1"
                     /translation="MIADGKMDVQLDVSKGSRVSRLEVIEGPTGRRNRSAAEKAKIAA
                     ESLVPGAEVATVARQHGVTRWQVYDWRRRLQRGRLSLPDSLVATFAPLMLDELPKTQA
                     SAVPVLVKLEIMVSDVVIRTEVKVEHLAGVILAMRASR"
     gene            619..972
                     /gene="tnpB"
                     /locus_tag="VNR65_05530"
     CDS             619..972
                     /gene="tnpB"
                     /locus_tag="VNR65_05530"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HWJ18173.1"
                     /translation="MITPGADLKVYVATRPVDFRKGHDGLAALVQQMFGLDPFSGAAF
                     VFRAKRADRVKILVWDRTGMVLVHKRLEGCKFVWPQVREGVMRISPAMFAALFEGLDW
                     RLVRPEGSRRPQLAG"
     gene            1050..2741
                     /locus_tag="VNR65_05535"
     CDS             1050..2741
                     /locus_tag="VNR65_05535"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWJ18174.1"
                     /translation="MDIAVLRNENERLKEQLARTQAALAESEEARQRLETIIVDMRRE
                     KFGAKSEKLRPDQFHLPLEDVEIAQGVLDAAQEKAAALIKGKSDGAPRPSNRNRGQLP
                     AHLPRVERIIEPKSTLCPCGCGEMAKIGEDVSERLDVIPAQLRVLVTRRPKYACRRCS
                     GAVAQAHAPEHMVPGGLPTEALIAQIIVAKFGDHLPFYRQSEIYARQGIRLDRATLGN
                     WTGRACFHLKPIADRMCAHLSAADRLFMDETTAPVLDPGRGQTKKGFFWAIAADDRGH
                     GGTGPPVVLFRYAPGRSGEHAERFLNGFRGRFLQCDGYDGYGRLTRIERPQGPWVLVH
                     CWSHLRRRFVKLVRNTKSPIAEAAVRQIATLYAVEATVRGASLKVRLAARREHSAPII
                     AALKPWLEKQLSMISSSSRLAEDIRYGLAHWVGLTRFLEDGRLELDTNPVENAIRPIC
                     LTRKNALFAGHEIGAENWALLSSIVATCRLNDVNPVAYLAETITAIIDGHPQSQIEDL
                     MPWRFRKTSVFRIISLMGTYFHVNPSQTYTTRPNPNSLVLAKKSPSQQCSGPLGF"
BASE COUNT          564 a          794 c          905 g          551 t
ORIGIN      
        1 acggttaagc tggtcgactt tgaacttgat ctgaaacggg caaagcgttc caagaaccag
       61 cccgctgaca actcctacat cattatcggt ttcgacacag aatataagac gcccgaccat
      121 cgtaagcgct tagctgatcc cctcgtgcgg agcatgacgg aagcgtgcaa ggctggcctc
      181 ggatcaacga ggtgtcggta tgattgcaga cggcaaaatg gacgtccaat tggacgtctc
      241 gaaagggagc cgcgtcagtc ggcttgaggt gattgagggg ccgactgggc gtcgcaatcg
      301 gtcggccgcg gagaaggcga agatcgccgc ggagagtctg gtacctggag cggaggtggc
      361 gacggtcgca cggcaacatg gcgtgacgcg ctggcaggtc tacgattggc ggcgtcgctt
      421 gcagcgagga cggctgtcac tgcctgatag cttggtggcg acgtttgcgc cgttaatgct
      481 tgacgaattg ccaaagacgc aggcatccgc ggttcccgtg ctggtgaagc tggagatcat
      541 ggtcagcgac gtcgtgatcc gaacggaagt caaggttgag catctggctg gtgtgatcct
      601 ggcgatgcga gcatcacgat gattacgccg ggagccgatc tgaaggtgta cgttgcgaca
      661 cgcccggtgg actttcgtaa aggtcatgat ggacttgcgg cgttggtgca gcagatgttc
      721 gggcttgatc cgttcagtgg ggcggccttt gtgttccgcg cgaaacgggc tgaccgtgtg
      781 aagattttgg tctgggaccg gacgggaatg gtgctggtgc acaagcggct ggagggctgc
      841 aagtttgtgt ggccgcaggt gcgcgagggt gtgatgcgta tatcgcctgc tatgttcgca
      901 gccttgttcg agggattgga ctggcgcctt gttcgtcctg aaggcagccg ccgcccgcag
      961 cttgcaggat agagcttatc actgcggcac agtgactcag gctctgcacc acacttggcg
     1021 ccgacgcgtg gcctgtgctt gaaaagctca tggacatcgc tgttctgcgc aatgagaatg
     1081 aacgcctgaa agagcaattg gcgcggacgc aggcggcgct tgccgagtcg gaggaggctc
     1141 ggcagcgatt ggagaccatc atcgtcgaca tgcgccgaga gaagttcgga gccaaatccg
     1201 agaagttgcg gcctgaccaa ttccatttgc cgctggaaga tgtggagatc gcccagggcg
     1261 tgctcgatgc cgcccaggag aaagcggcgg cgctcatcaa gggcaaatcc gacggtgcgc
     1321 ctcgtccatc caatcgcaat cgcggtcagc tgccggctca tttgccaagg gtcgaacgga
     1381 ttatcgagcc gaagagcacg ctgtgcccgt gcggctgcgg cgagatggca aagatcggcg
     1441 aggacgtgag cgagcggctg gacgtgatcc cggcgcaatt gcgggtgctg gtgacacgtc
     1501 gcccgaaata tgcctgccgc cgctgttcag gcgccgtggc gcaggcgcat gcacccgagc
     1561 atatggttcc cggcggcctg ccgaccgagg cgttgatcgc tcagatcatc gtcgcgaagt
     1621 tcggcgatca cttgccgttc taccggcagt ctgagatcta cgcccgccag ggcatccggc
     1681 tggatcgagc gacattgggg aactggacag gccgagcctg cttccatctg aaaccgatcg
     1741 ccgaccgcat gtgtgcgcat ctgtcggccg cggatcgtct cttcatggat gaaaccacgg
     1801 cgccggtcct cgatccgggg cgcgggcaga caaagaaggg gttcttttgg gcgattgctg
     1861 ccgatgatcg cggacatggc ggcaccggtc cgccggtcgt gctgttccgc tacgcgccgg
     1921 ggcgaagcgg cgaacatgcc gagcgattct tgaatggctt ccgtgggcga ttcctgcagt
     1981 gtgacggcta tgatggatat ggtcggctca cgcgcatcga acgaccgcaa gggccatggg
     2041 tgttggtgca ttgctggagt cacttgcgcc gccgcttcgt gaagctggtg cgcaacacga
     2101 aatccccgat cgcggaagct gccgttcggc agatcgccac gctctatgcc gttgaggcga
     2161 cggtgcgtgg cgcatcgctc aaggtgagac tggccgcgcg acgggagcat tccgcgccga
     2221 tcatcgccgc cctgaaaccg tggttggaaa agcagctgtc gatgatctcc tcgagctcca
     2281 ggctggctga agacatccgc tatggcctcg ctcattgggt ggggctcaca cgcttcctcg
     2341 aggatggccg cctcgaactc gacaccaatc cagtcgaaaa cgctatcagg ccgatctgcc
     2401 tgaccagaaa gaatgccctg ttcgccggcc atgagatcgg agccgaaaat tgggcgctgc
     2461 tctcatccat cgttgccacg tgcaggctca acgacgtcaa ccccgtcgct tacctcgccg
     2521 aaaccatcac ggctatcatc gacggtcatc cacaaagcca gatcgaagac ctcatgccct
     2581 ggcgattccg aaagacgtca gtctttcgca taatctccct catgggaacg tacttccacg
     2641 taaacccgtc gcaaacgtac acaacccgcc ccaatcctaa tagcctcgtt ttggctaaaa
     2701 aaagcccctc gcagcaatgt tcagggcctt tgggcttcta acgacctctg gtagcctcag
     2761 gttctgaggg caccatggag gacgacaatg acgacaggtc cattatcgag aaga
//
LOCUS       DATMGX010000346         2417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_329536, whole genome shotgun sequence.
ACCESSION   DATMGX010000346 DATMGX010000000
VERSION     DATMGX010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329536"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..613
                     /locus_tag="VNR65_05540"
     CDS             101..613
                     /locus_tag="VNR65_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ18175.1"
                     /translation="MKSRQIGRTRISGTEWNQNSYLLLNLTRECGNPRAWGIGAVQDL
                     LAILVVEDDPLIQSVVEETLMEGGFEVAIVSSGQRAVELLDGTEAKYRALVTDVNLGR
                     NKMDGWEVAKYAREIEPEFPIVYMTGDSAADWGSKGVPNSIVLIKPFAPAQLLTAVSQ
                     LLNSGATPTK"
     gene            718..906
                     /locus_tag="VNR65_05545"
     CDS             718..906
                     /locus_tag="VNR65_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18176.1"
                     /translation="MNLWTGAAMLVAAFLLIWAGRPNKAGVHPRFLRFDAALVLYTPV
                     ILAFTAMGAAAVISSIWK"
     gene            complement(1023..>2417)
                     /locus_tag="VNR65_05550"
     CDS             complement(1023..>2417)
                     /locus_tag="VNR65_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWJ18177.1"
                     /translation="DDAGTPSDNAALGRIDSRIDSLMGDARRHLRQASGALISELEAR
                     DFAAVRRSLAQADFLRDELDNNFDAVRNEMLKVSYGAIATIRSEQTQAVLTSAVVTLL
                     SAIVGLIFANLVSGGIIRSVRRLLEGTRAVEAGDLDQSIDVTTEDEIGQLAGGFNRMV
                     MQLRDNQRVRETFGKYIDPRVVEGLIDRPNLTAAEGQRRVMTVLFCDMKGFTSLSEGM
                     TPQGLVKVMNRYLSIMSEPIRTNRGIIDKYIGDGIMAYWGPPFVGEADHARFACLATM
                     EMIERIATLRQEIPEILGVRGTPMERCDLRIGVATGEALVGSIGSDFMMSYTVMGDVV
                     NLASRLEGANKLYGTRNLVSERTITATGGTFEVREVDRVVVAGHTHSEVVFEILGSKG
                     ELTPQQVLSRDRYLEGLAAYRERRWDDALRLLNAALEAMPGDGPSTALLTRVEDLKAN
                     PPAQDWDGSWHIEK"
BASE COUNT          529 a          711 c          654 g          523 t
ORIGIN      
        1 aacatagtgg ctgaaccggc gcggtattcc tagcatacta acctcgggaa aggatgactg
       61 acgataaagc aaggatcatg cccacggcaa tcaaccctcg atgaaatcgc gtcagattgg
      121 acggactcga atttcaggaa ccgagtggaa ccaaaatagc tatttgcttc ttaacttaac
      181 tcgggaatgc ggcaacccgc gcgcatgggg gatcggagcc gtgcaagacc tacttgccat
      241 tctggtcgtc gaagatgacc cactgattca gagcgtcgtg gaggagaccc ttatggaggg
      301 aggatttgag gtggccattg tgtcgtcagg tcagcgtgct gtggaattgc ttgacggcac
      361 cgaagcaaaa taccgagcac tggtgaccga cgtcaatctt ggacgaaaca agatggacgg
      421 atgggaggta gcaaagtacg ccagagaaat cgaaccggaa tttcctattg tttatatgac
      481 cggcgacagt gccgcggact ggggttcgaa aggagttcca aacagcatcg ttttaattaa
      541 gcctttcgct cctgcccaac ttcttacagc cgtatcccaa ctgctaaata gcggcgccac
      601 gccgacaaag taggccgttt cggttggcgc gaacgccaag gttacaacca ttggctactt
      661 gtaagaccgg agctttagga ttctgctgtg accacaaaca ctcaaagggg cccacaaatg
      721 aacctatgga ctggagctgc gatgctggta gcagcatttc tcctgatctg ggcaggccgc
      781 cccaataagg ctggcgttca ccccagattc ctacggttcg acgcggcgct agttctttat
      841 acgccggtta tcctcgcatt taccgcgatg ggggcggctg cggtgattag ctcgatttgg
      901 aagtgaggcc gcctcagttg gccgcctctt gcgaccgcac gcctttctgc agcgcgatca
      961 ggtaacttgc caccgcgcgc tgattagcca gcctcgcgcc aacccggcga atgctttgac
     1021 gactattttt cgatgtgcca cgacccgtcc cagtcttggg ccggtgggtt tgccttgagg
     1081 tcttcgacgc gcgtgagcag agccgttgac ggcccgtcgc cgggcatcgc ctcaagcgcg
     1141 gcgttaagca agcgaagcgc atcgtcccag cgtcgttcgc ggtaagccgc aagcccttcg
     1201 agatacctat cccgtgataa cacttgttgt ggcgtgagtt cgcccttgct tcccaggatc
     1261 tcaaaaacga cttccgaatg ggtgtgacct gctaccacta cccgatcgac ctctcgaacc
     1321 tcaaatgtgc cgcccgtcgc ggtgatggtt ctctcggaga cgagattgcg ggtaccatac
     1381 aacttgtttg cgccttccaa ccgtgaggcg aggttcacca cgtcgcccat gacagtataa
     1441 ctcatcatga aatcagagcc gatgctaccg accaacgcct ctcccgtcgc aacaccgata
     1501 cgcaaatcgc atctctccat gggtgtaccg cgtacgccaa gtatctcggg gatttcctga
     1561 cgcaacgtcg cgatgcgttc gatcatttcc atggtggcca gacatgcaaa ccgggcgtga
     1621 tcagcctcgc cgacgaacgg agggccccaa taggccatga tgccgtcacc gatatacttg
     1681 tcgatgatcc cccggttcgt ccggatcggt tcggacatga ttgaaaggta acggttcatg
     1741 accttgacca gaccttgcgg cgtcatgccc tcgctcaggc tggtgaaccc tttcatgtcg
     1801 caaaacaaaa ccgtcatcac ccgacgctga ccttccgctg cggtcaggtt cggacggtca
     1861 atcaaccctt cgacgacacg tggatcgata tatttgccga atgtctccct aacgcgctgg
     1921 ttgtcgcgta gctgcatgac catccggttg aacccaccgg ccagttgtcc aatttcgtct
     1981 tccgtcgtta catcgatgga ttgatcgagg tcgcctgcct cgaccgcccg tgtgccttct
     2041 agcagtcgcc ggaccgagcg aatgatgccg ccgctaacta ggttggcaaa aatcaatccg
     2101 acaatcgcgg agagaagcgt cacaaccgct gaagttagaa ccgcctgcgt ctgctccgac
     2161 cttatcgttg cgatggcccc atagctcacc ttcagcatct cgttgcgaac ggcgtcaaaa
     2221 ttgttatcga gttcgtcgcg gagaaagtct gcttgcgcaa ggctacgtct cacggccgca
     2281 aaatctcgcg cctcaagttc cgaaatgagg gcccccgacg cctgcctcag atgacgacgg
     2341 gcatcgccca tcaagctgtc gatacggctg tcgatccgac ccagcgcggc attgtccgac
     2401 ggggtaccgg cgtcgtc
//
LOCUS       DATMGX010000347         4512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_386837, whole genome shotgun sequence.
ACCESSION   DATMGX010000347 DATMGX010000000
VERSION     DATMGX010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386837"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1378
                     /gene="pstS"
                     /locus_tag="VNR65_05555"
     CDS             341..1378
                     /gene="pstS"
                     /locus_tag="VNR65_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWJ18178.1"
                     /translation="MKIKAWRRAVLLLLAGIGFTFCGDGVNSQEAADVRLQGAGASFP
                     FPIYSKWFKVYSVAHQNVQIDYQSIGSGAGVKNFIDKTVDFAASDKAMKPKEIARVAA
                     GVQLLPLTAGNIVLAYNVPGITDLRMSRAAYAGIFLGKVKKWNDPAIAKTNPGQKLPD
                     SAINVLVRADASGTTFVFTQHLSAISKEFALNPGTNDMPAWPVGTRSKGNEGVTASIQ
                     TTPGSIGYIEFGYAMIQKLPMAQLENKSGNFVAPSTASGQAAIASAEVQDNLIAWVPD
                     PTVKDAYPIATFTWFLSYKKYPDPNKAEVLRNLLMYCLTDGQKESEALGYLPLPDAVA
                     AKVKAAVQNVQ"
     gene            1456..2478
                     /gene="pstC"
                     /locus_tag="VNR65_05560"
     CDS             1456..2478
                     /gene="pstC"
                     /locus_tag="VNR65_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWJ18179.1"
                     /translation="MPSKTGISRPPSRAQIVTDTAFRGLCRGFALLAVVLAVFIVLKI
                     VIAAMPAIQQYGFDFLTGQVWDPNKKQYSILPEIWGTLASSFFALIVATVFGVMAAVF
                     LAEGFVADYVFALLKIFGLQFHPVWRKLPEQADMVLRNLIELLAAIPSVVYGLWGLFV
                     VIPLVRPAAHWLHANMGWVPFFSTDLSGPGMLPAVIVLAIMILPTITAISTGAMIAVP
                     TRLKMAAYGLGATRWETLLGVTVPTASRGIFGGVVLAFGRALGETMALAMLLGNENVI
                     SVSLFSPANTLAALLANAFPEANSKDVGVLMYVATVLLVITLLVNVAGAYVLQRASSG
                     LEGDKS"
     gene            2475..3428
                     /gene="pstA"
                     /locus_tag="VNR65_05565"
     CDS             2475..3428
                     /gene="pstA"
                     /locus_tag="VNR65_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWJ18180.1"
                     /translation="MTTVDARVDADVPRESEAHETDPFHGVDFSKLERSLRKPRTFVS
                     SALSGIATAMTVFALIPLFSVLGMLLWRGGRELSIAAFTQLPPAPLELGGGFGNAIVG
                     TLVMVGMALLISLPVGIMSAIFLAQSKPDNYLAATVRFVAKVLTGFPSVLAGVFAYGA
                     VVLVTGGYSALAGAVALSILMVPTILLTAEDAIRMVPKRMKEAAIGIGATDTQTVWMV
                     LLPTALPGILTGVMLAVARGAGETAPLLFTALFSNYWILWHGHLKLMEPTASLAVLIY
                     NFSGMPFPNQVELAWAAALVLVLLVLAANLIGQSLSHRQVQ"
     gene            3444..4274
                     /gene="pstB"
                     /locus_tag="VNR65_05570"
     CDS             3444..4274
                     /gene="pstB"
                     /locus_tag="VNR65_05570"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWJ18181.1"
                     /translation="MSSPKAASPESSPKNAEQDPSLMMDCNIKELFYGTFKAVRDTRI
                     PIQKNAITAFIGPSGCGKSTVLRCLNRMNDLVRGFRFEGYVNYRGKDIYGPTVDPVAV
                     RRYIGIVFQQPNPFATSVYNNVAFGMRVNGFKGDKRAKVEEALKAAGLWDEVKDKLND
                     SGLSLSGGQQQRLCIARAIATEPEVLLMDEPCSALDPVATKRIEELMQELKTQFTIAI
                     VTHNLQQAQRVSDKTGFLYVDTSEGARTGYLVEFGDSKQIFNDPKEKQTQDYIKGVFS
                     "
BASE COUNT          957 a         1238 c         1304 g         1013 t
ORIGIN      
        1 aatcgacagc gtagtgggga tccaactcaa cgctgacgcc cggcgtgtat tcgatatata
       61 gtgtatatta taaaaaaaac agtaaaatag caccaatact tatctaacaa agatcttgta
      121 tgaagacgtt cacgggtttt gtatttgtca tataagcttc ataattccaa gctttgtgtt
      181 tgtcatataa gcttcataat tcagagtgct ttgcgtaaac agtaaaacac tgagtatttt
      241 ccgagtcttt ctcgtcaacc tcatttatgt gactttttga aagagttcac cagcggtgcc
      301 accgtttcat ttaaagccag cgtggttaga gaggctactt atgaaaatca aagcttggcg
      361 ccgcgccgtt ctgctattgc tcgctggtat cggctttacg ttctgtggag acggcgtcaa
      421 ttcccaggaa gctgccgacg ttagactaca gggagccgga gcgagcttcc cgttcccaat
      481 ctattcgaag tggttcaagg tttatagcgt cgctcatcag aacgtacaga ttgactatca
      541 gtcgataggc agcggcgccg gggtaaagaa cttcatcgac aaaacagttg actttgctgc
      601 cagcgacaaa gctatgaagc ccaaagagat cgcacgcgtc gcggccggcg tccaacttct
      661 tcctctgacg gccggcaaca tcgttctagc ctacaacgtt ccgggcatca cggatttgag
      721 gatgtctcgc gccgcatacg ccggcatctt ccttggcaag gttaagaagt ggaacgaccc
      781 ggccatagca aaaacgaatc ccgggcagaa gctgccggat agcgccatca acgtgcttgt
      841 gcgtgctgac gcgagcggca ctacattcgt atttacgcag catctgagcg cgatcagcaa
      901 ggaattcgcc ctcaacccgg gaacgaacga catgccagca tggccggtgg gcacccgatc
      961 gaaggggaac gaaggggtta ctgccagcat ccagaccaca cccggctcca tcggctatat
     1021 cgagttcggc tacgcgatga tccagaagct gccgatggcg cagctcgaga acaagtctgg
     1081 aaattttgtg gctccatcca cggcctcggg ccaggcggcg atagcttccg cagaagtgca
     1141 ggacaacctc atcgcgtggg ttccggatcc aacggtgaaa gacgcctatc ctatcgcgac
     1201 cttcacatgg ttcctttcct ataaaaaata tcccgatccg aataaggcgg aagtgctgcg
     1261 caatctactt atgtattgtc tcacagatgg tcagaaagaa tcggaagcac tcggctacct
     1321 cccgttgccc gatgccgtgg ccgccaaggt gaaagccgcg gttcagaacg tgcaataacg
     1381 agccactgtg tgcacgccgg aatttcttgc agatctggcg atacgagccc gataaccgaa
     1441 gcgaaggatc gacggatgcc atccaagacc gggatttcca ggccgccatc gcgggcccag
     1501 atcgtcactg ataccgcgtt ccggggcctg tgccgggggt tcgcgttgtt ggccgtcgtc
     1561 cttgctgttt tcatcgtgct caagatcgtt atcgccgcga tgcccgccat tcaacaatat
     1621 gggttcgact ttcttacggg gcaggtttgg gacccgaaca agaagcaata ctcgattctt
     1681 ccggaaatct ggggcactct ggctagttcc ttttttgcgt tgattgtggc aacggtgttc
     1741 ggggtcatgg cggcggtttt cctcgccgaa ggcttcgtcg ctgactatgt gtttgcgttg
     1801 ctcaagattt ttgggctgca gttccaccca gtttggcgca agttgcccga gcaggcggac
     1861 atggtccttc gcaacttgat cgaactgctt gctgcgattc cgagcgtcgt ctatggcctg
     1921 tgggggctgt tcgtcgtcat ccccttggtc cgtccggccg cccactggct gcacgcgaac
     1981 atgggctggg taccgttttt cagcacggac ctgagcggtc cgggaatgct gcccgccgtg
     2041 atcgtgctcg cgatcatgat tcttccgaca ataacggcaa tcagcactgg cgccatgatc
     2101 gcggtgccga ccaggttgaa gatggctgcc tacgggctgg gtgcgacgcg atgggagacg
     2161 cttctcggcg tgaccgtccc tacagcgtca cggggcattt tcgggggcgt ggtgttggcg
     2221 ttcggccgcg cgcttggcga gaccatggcg ctcgccatgc tgttgggaaa tgagaatgtt
     2281 atcagcgtct cgttgttctc gccggccaat acgctggcgg ctcttctggc gaacgcattc
     2341 ccggaagcga actccaagga cgtcggagtc cttatgtacg tggcgaccgt gctgctcgtc
     2401 attacgcttc tggtgaatgt cgcgggcgcc tatgtcttgc agcgcgcttc ctcggggctg
     2461 gaaggagaca agtcatgact acagtggacg ccagggtcga cgccgatgtg ccgcgagagt
     2521 ccgaagcaca tgaaactgat ccatttcatg gcgtggattt ttcgaagttg gagcgatcgc
     2581 tgcggaagcc gcggacgttc gtgagcagtg cgctgagcgg gatagcgacg gcgatgacgg
     2641 tgttcgcgct gattccactg ttctctgtgt tgggtatgct gctctggcgg ggagggcgcg
     2701 agcttagcat cgccgccttc acccagcttc cgccggcccc gctcgagctg ggcggcgggt
     2761 ttggaaacgc catcgtcggc accttggtga tggtagggat ggcgctgctc atcagcctcc
     2821 ccgtcggcat tatgagcgcg atcttcctcg cgcaatcgaa gcccgacaat tacctagcgg
     2881 cgacggtccg cttcgttgcc aaggtgctga ccggattccc ttcggtcctg gctggcgtgt
     2941 tcgcgtacgg ggccgtggtt ttggttacgg gcggttattc ggccctcgcc ggagccgtgg
     3001 cgctttcgat cttgatggtg cccaccattc ttctcaccgc cgaagacgcc atccgcatgg
     3061 tgccgaaaag aatgaaggag gcggcgatcg gaatcggcgc caccgataca cagaccgtct
     3121 ggatggttct actgccgacc gcccttccgg gcatcctcac aggagtgatg ctggcggtcg
     3181 cgcgtggcgc cggcgaaacc gctcccctgc tgtttacggc gttgttcagt aactattgga
     3241 tcttgtggca tggccatttg aaactcatgg agccaacggc ctcgttagcc gtcctgatct
     3301 ataatttctc cggcatgccg tttcccaacc aagtggaatt ggcctgggcc gccgccctgg
     3361 ttctggttct attggttctt gcggccaatc ttatcgggca aagcctatcg caccgacagg
     3421 ttcaatgaga gaagggatca cctatgtcga gtccaaaggc tgccagtcct gaatcaagcc
     3481 caaagaacgc ggagcaagat ccgtcgctga tgatggactg caatatcaag gaactctttt
     3541 atggaacctt caaggccgtt cgcgacacga ggattcccat ccagaagaac gcgataactg
     3601 cctttattgg accgtcgggc tgcggcaaga gtacggtgct ccgatgccta aacaggatga
     3661 atgatcttgt ccgtggtttc cggttcgaag gatatgtcaa ctaccgcggg aaagatatct
     3721 acggacccac agtcgatccc gtggccgtgc gccgctatat cggcatcgtg ttccagcagc
     3781 cgaacccatt tgcaaccagc gtttataata atgtcgcgtt cggaatgcgg gtgaacggct
     3841 tcaaaggtga caagcgcgcc aaggtagagg aagccctaaa ggcggcgggg ctgtgggacg
     3901 aggtgaaaga caagctgaac gacagcggcc tctcgctttc gggcggccag cagcaacggc
     3961 tttgcatcgc cagggccatc gctacagagc cggaagtgct gttaatggac gagccttgct
     4021 ccgcgctcga cccggtcgca acgaagcgga tcgaggaact catgcaggag cttaaaaccc
     4081 agtttacgat cgcgatcgta actcacaatc tccagcaggc tcagcgagta tccgataaga
     4141 caggcttctt atatgtcgat acctccgagg gcgcccggac cgggtacctc gtcgagttcg
     4201 gcgactccaa gcagatcttc aacgatccta aggagaaaca aacgcaagat tacattaagg
     4261 gagtgttcag ctgacttaga gtccaaacct atgaagcttt cgattggtgc gaaattgggc
     4321 tccgttgcga ttcggctggc gagcacgaga cgaacttccc gataattcac caccgaaaac
     4381 tactcccgta ggatctgggg cagaaggggt gtcgatcatg aaagcgcatc ttatcgaagg
     4441 cggcatcgga tcgctggcgg cagcggcctt catgatccgg gacggcggca tcagcacatt
     4501 ccgagcctgc ca
//
LOCUS       DATMGX010000348         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_390878, whole genome shotgun sequence.
ACCESSION   DATMGX010000348 DATMGX010000000
VERSION     DATMGX010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390878"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VNR65_05575"
     CDS             <1..630
                     /locus_tag="VNR65_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWJ18182.1"
                     /translation="AVLLILLVFSLASWAIIFSKWGLFRRARVQSNRFMRAFRKSERL
                     QDVAAVAEQFKPSPLIAVFDGAYEELRKQAPYPIRMIALQRATQIASSEELSRLESRL
                     PWLATTGAVTPFIGLFGTVWGIIDAFHGLGTAGGATLRAVAPGISEALITTAAGLFAA
                     IPAVIAYNMFTQHIREFGARMDDFSMELINEVERAQTGSARAVESVERR"
     gene            630..1058
                     /locus_tag="VNR65_05580"
     CDS             630..1058
                     /locus_tag="VNR65_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWJ18183.1"
                     /translation="MAFTNSRGRTQTSLADINVTPLVDVVLVLLIIFMVTAPILQSGI
                     EVAIPHTRTVKEITEERLVITIDRQQHVFLNNQPVNINQIGAQLHEKIRDPEGQSVFV
                     RADENVPFGAFATVMDAVKQSGITNVSIVTQPLEKNGSRR"
     gene            1042..1830
                     /locus_tag="VNR65_05585"
     CDS             1042..1830
                     /locus_tag="VNR65_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWJ18184.1"
                     /translation="MAADVDIFPTGGNVRAPLIGSVTLHVALFALALFGYLIPSSRGE
                     NWGGTGGNGGAMSATLVSSIPLPKPPTETKNVLANESKGLTQSPPKEIPKEEPKAIPI
                     PARDAKRKGPRESEQKKPPPKEIAKVEENRIPFGQGGPVSGPYGSFSAGGAKGGLSFT
                     GGSGDFGSRFGWYVDGVRRKVSENWLKYEVDPNIDTARRVYIYFEITRSGQPENIRVE
                     QSSGIPSLDQSAARALQRIDTFGPLPPEYAGRYVAVEFWFDYRR"
BASE COUNT          398 a          577 c          636 g          395 t
ORIGIN      
        1 gctgtcctct taattctgct ggtgttcagc ctggcatcgt gggccatcat cttctccaaa
       61 tggggcctgt tccggcgtgc gcgggtgcag agcaatcgct tcatgcgcgc gtttcgcaag
      121 tcggagcgac tgcaggatgt cgccgccgtc gcggaacaat tcaagcccag cccgctgatc
      181 gcagtgttcg acggcgccta tgaagaactg cgcaagcagg ccccgtatcc cattcgcatg
      241 atcgcgctac agcgcgctac tcagatcgca tcctctgagg aattgagccg cctggagagc
      301 cgcttgccct ggctggcgac cacgggagca gtcactccct tcatcggcct gttcggaacg
      361 gtgtggggca tcattgacgc ctttcacggt ctcgggaccg ccggcggggc caccctgcgc
      421 gccgtcgcgc ccggcatttc cgaagccctg atcacgaccg cggccggctt gttcgccgct
      481 attcccgcgg tgatcgccta caacatgttc acccagcaca ttcgcgagtt cggcgcccgc
      541 atggacgact tcagcatgga gctgatcaac gaagtcgagc gggcacagac cggcagcgcg
      601 cgcgcggtcg agagtgtgga gcggcggtaa tggcgttcac caattcacgg ggccggaccc
      661 agacgtcgct ggccgacatc aacgtcactc cgctggtgga cgtggtgctg gtgttgctga
      721 tcatcttcat ggtcaccgcg cccattttgc agtcaggcat tgaagtcgcg attccccaca
      781 ctcggaccgt aaaggagatc accgaggagc gcctggtgat caccatcgat cgtcagcagc
      841 acgtcttcct caataaccag cccgtcaaca tcaaccaaat cggcgcccag ttgcatgaga
      901 agattcgcga ccccgagggc cagtcggtgt tcgttcgcgc cgacgaaaac gtgccctttg
      961 gcgccttcgc gaccgtgatg gacgccgtga agcagtcggg catcaccaac gtgagcatcg
     1021 tgacccaacc gctggagaag aatggcagcc gacgttgaca tctttccgac cgggggcaat
     1081 gttcgcgctc ctttgatcgg ctcggtgacc ctgcatgtcg cgctgttcgc cttagctttg
     1141 ttcggctatc tgattcccag cagccggggc gagaattggg gaggtacggg tggaaacggc
     1201 ggcgcgatga gtgccacgct ggtgagcagc atcccgttgc ctaagccacc gaccgagacc
     1261 aaaaacgtgc tggccaacga atcgaagggg ttgacgcaat cgccgccgaa ggagattccg
     1321 aaggaagaac cgaaggcgat ccctattccc gcgcgcgacg ccaaaaggaa gggaccacgg
     1381 gagtccgagc agaagaagcc gccgccaaaa gaaattgcca aggtggagga aaaccggatt
     1441 ccgtttggcc agggagggcc ggtcagcggg ccttatggat ccttctcggc gggcggagcg
     1501 aaaggcggac tcagctttac cggaggcagc ggagatttcg gcagccgctt cggctggtat
     1561 gtggatggag tgcgccgcaa ggtgtcggaa aactggttga agtatgaagt ggatcccaac
     1621 atcgataccg cgcgcagggt ttacatttat tttgagatca cgcgttcggg gcagccggaa
     1681 aacatacgcg tggaacagtc gagcggcatt ccgtcgctcg atcagtcggc ggcgagggcc
     1741 ttgcaacgca tcgatacgtt tggtccccta cctccggagt acgcaggccg ttatgtcgcc
     1801 gtcgagttct ggttcgacta tcggcggtaa ggtggggttg gattgttcag ctgtttcggt
     1861 agttcgaaga tagcaagctt tgaaagggcg cggcttcaca gggtgcggaa caactctgtg
     1921 ggaggctttt ggtggaagct tcctgcttta gcagggggtg ctgaacttta gtccagcgga
     1981 aaaggagccg attttgcaat gggctt
//
LOCUS       DATMGX010000349         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_275264, whole genome shotgun sequence.
ACCESSION   DATMGX010000349 DATMGX010000000
VERSION     DATMGX010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275264"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VNR65_05590"
     CDS             <1..947
                     /locus_tag="VNR65_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HWJ18185.1"
                     /translation="LHEKAFRRLGGCPRLVVLDNLREGVIVADIYDPTLNPLFRDVLA
                     HYGVVAMPCRIKDPDRKGKVEAGVGHAQKTPLKGQRFESLEEAQAYLDRWEERWADTR
                     IHGTTKRQVAAMFAEEKPSLLPLPLEPFRYYQYGERVVHLDGCVEVEAAYYGLPPGWI
                     GRSVKVQWDALHVRILHPGTNQLLREHIRQKRGWYRIKNEDYPKKMPLSTAQLLRRAG
                     HAGRQIGVLSVAIYQRQGEAGIRRILGILSLAKKYGTAALEDACAAAIEIGVPEYRFV
                     RRYLERRPQLSLRQVDPLIRELTEYRDFINLKLKEQAE"
     gene            944..1699
                     /gene="istB"
                     /locus_tag="VNR65_05595"
     CDS             944..1699
                     /gene="istB"
                     /locus_tag="VNR65_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HWJ18186.1"
                     /translation="MNLIELNRALRQLRLGGIAAVLETRLHQAQAEPMAPIDFLSCLV
                     SDELTRRTDRLLERRRKQAQFRDPQKTLDNFDFNFNKKMNRSMVFDLATAAFIGRRED
                     SLFLGPPGTGKSHLAQAIGQAVIGQGYRVLYREAHKLLEEITEATIDGTRKEHMELLA
                     TVPLLIIDDLGMRKLAPTAAEELLEIVMRRHERASTMVTSNRPVEDWGKLLGDSAAVT
                     AMLDRLLHHGHILKCGPRSWRTKTDLPDQEKMG"
     gene            complement(2092..>2339)
                     /locus_tag="VNR65_05600"
     CDS             complement(2092..>2339)
                     /locus_tag="VNR65_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18187.1"
                     /translation="SLRDGEFTCCDLCGIGHELFNLVLDLVSDAAVHFFLLIGQSTGG
                     IWVYDIPVQNAEREGKYRIPFLGRITDGDHMAERFFQ"
BASE COUNT          573 a          655 c          652 g          459 t
ORIGIN      
        1 aactgcatga gaaagccttt cgtcgattag gtgggtgtcc gcgtctggta gttctcgata
       61 atctgcgcga aggagtcatc gttgcggata tttacgatcc cacactcaac ccgttgtttc
      121 gagatgtgct cgcacactac ggcgtcgtgg ctatgccgtg ccggattaaa gatcccgatc
      181 gcaaaggaaa agtggaagca ggtgttggac acgcgcagaa gactccgctc aagggtcagc
      241 gcttcgagag tctggaagaa gcgcaggctt atctggatcg ctgggaagaa cgttgggccg
      301 atacgcgcat tcatggcacc acgaaacggc aagtggcagc tatgttcgcg gaggaaaaac
      361 catcgttgct tcctcttcca ctcgaaccct tccgctacta ccagtatgga gaacgcgtcg
      421 ttcatctcga cggctgtgta gaagtcgaag ctgcttatta cggattacca ccgggatgga
      481 tcggacgaag cgtcaaagtg caatgggatg cgctgcacgt gcgcatcctg catcccggta
      541 cgaatcaact tctccgcgag catattcgcc agaaacgcgg ctggtatcgc atcaagaacg
      601 aagactaccc aaagaaaatg ccgttgagca ccgcacagct attgcgtcgt gcgggccacg
      661 ccggaaggca gatcggcgta ctgtctgtgg ccatctatca gcgacaaggc gaggcgggca
      721 ttcgccgcat cctggggatc ctctccttgg ccaagaaata cggtacggcg gctcttgaag
      781 atgcctgtgc cgcggccatc gagatcggtg tgccggagta tcgcttcgta cgccgttatc
      841 tcgagcgccg cccacaactg agcctccggc aggtcgatcc gctgatccgt gaactcaccg
      901 agtatcgtga cttcatcaat ctcaaactca aggagcaagc agaatgaatt taatcgaact
      961 caatcgtgcg ttaagacaac tgcgcctcgg cggcatcgcc gccgtgcttg agacgcgtct
     1021 gcaccaggcc caggcggaac cgatggcccc catcgacttc ctctcctgtc tggtgtcgga
     1081 cgaactcacg cggcggaccg accgactgct ggagcgacga agaaaacaag cacagtttcg
     1141 cgacccccag aaaactctcg acaacttcga tttcaacttc aacaaaaaga tgaatcgaag
     1201 catggtcttc gatctggcga ccgcggcgtt catcggtcgt cgggaagact cacttttcct
     1261 aggtccaccc ggcaccggga aaagtcactt ggcccaggcc atcggacaag cggtgatcgg
     1321 acagggctat cgagtgctct atcgcgaggc ccacaagctg cttgaggaaa tcacggaagc
     1381 caccatcgat ggaactcgca aagagcatat ggaactcctg gccaccgtgc cgttgctgat
     1441 catcgatgat ctgggcatgc gcaagctagc gcccacagcg gcagaagaac tgctggaaat
     1501 cgtgatgcgc cgtcacgagc gcgctagcac catggtcacc tcgaaccgcc ctgtggaaga
     1561 ctggggcaaa ctgctcggag acagcgcggc cgtcaccgcc atgctcgacc gcctgctaca
     1621 ccacggtcac atcctgaaat gcggaccgag gagctggaga acgaaaacag acttgccgga
     1681 tcaagagaag atgggataaa agaaccttgt cccgggccac atccgttggc cggttttgac
     1741 atgatccccg ttggccgctt ttgacctgac ccctgaggtg cagatcatcg cttcggatag
     1801 tgctctccac cccgacacct aaagttccac ggcttgtcgc tgagttcgac cgaatccact
     1861 tcggcgaagt ggagagaaat gacaaggcat aagccaaagg ttctattcct gtcgacgggc
     1921 aacgccacta gaagtttgat cgctgagggt tttctacgaa gcctcacggg cgaccgattt
     1981 gatgttgtga gtgtgggggt cgaacgcagc gcctcatatc ccatggcaag tgaagtgatg
     2041 aaggaagccg gaatcgacat ttcgagccag aagacgaaga gcgtcgctca atcattgaaa
     2101 gaagcgttcg gccatgtgat caccatctgt gatacggcca aggaaaggaa tccgatattt
     2161 cccttcgcgc tcggcgtttt gcactggaat atcatagacc caaataccgc cggtggattg
     2221 gccgatcaga agaaagaagt gtaccgccgc gtccgagacg agatccagga caaggttaaa
     2281 caattcgtgg ccgataccgc acagatcaca acaagtgaac tctccatcgc gtaaggaat
//
LOCUS       DATMGX010000350         2914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_355170, whole genome shotgun sequence.
ACCESSION   DATMGX010000350 DATMGX010000000
VERSION     DATMGX010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355170"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VNR65_05605"
     CDS             <1..846
                     /locus_tag="VNR65_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWJ18188.1"
                     /translation="GLRKQELEQARIEFERWQDEYKRMKFLVERKSLPPNDFQKIEAA
                     YNASKERYEMAVEGTRKEDIATAAAQSHAADAQAAEERKRLDDTRLLAPIFGNVSMRR
                     VDPGQTVAAGMPVFSIVELNPVKVRVGVPEAEIAKVRQGAAAEITVPSLDGRCFKGKV
                     AIIGVASEPTSRTYTVKILVPNPGPVLLAGMVAEARIFGPAKLRPLTIPAEAVVPDAQ
                     GAPNVYIYSPDRKRVYGRRVEVGTPIGNEVEIHSGLQGDEQIVVVGQQKVREGSLVEI
                     AGGAR"
     gene            843..>2914
                     /locus_tag="VNR65_05610"
     CDS             843..>2914
                     /locus_tag="VNR65_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWJ18189.1"
                     /translation="MNPVRASLRYPQVTLALTIMLVASGVYALLRMPRREDPKIHVRI
                     GIVAAFYPGASAAEVENQVTQKVEQRLFKIEGVRREKTYSTSMNGVMFINLNLEDNLQ
                     DTEKFWSKLRLDMAELKKTDLPDGVEGPIVDYDFGDTVAVLLAIHGDHYGYRELKDYA
                     ERIESSIRRIRSVSKVKRIGEQKEEIDVSSSLSRLSQYSVTPEKVFKALEGRNMVHYA
                     GAVPTEHGKPPIQTTGAFEGEEQIRRVMIDVTRTGQPVYIGDLANVDRVYKDPTQYAR
                     SRGERAMLLSVEMQEGNNIVDFGKELHASLDALRPLLPPDLKIDFVADQPTVVAERVK
                     SFIREFGISIASVILVTMLLLPFRVALVSAVAIPVTISATFGVLNAFGIELHQVSIAA
                     LIVVLGMVVDDAIVIADNYIELLDHGIAREEAAWRSATELAIPVLTATLTIICSFLPF
                     LLLSGATGEFIRALPLTVAIALATSFLVAMLLTPMLSQFFIRKGLHNPEDSSAKKRRG
                     PLEIMQAIYNRVIVVAMRWKVAAVAFGVFAVAAGAFMLHIVPERFFPFAERDQFVIDV
                     WLPEGWKVEATESAVKRIEDVLSHEKEVVNFTSFIGSSFPRFYYNVNPQLPDKNYAQM
                     LVSTKSAEVTPELVRALRPQLAQAAPEARVFLSELQQGPVQEAAIEVRLTGDDQAVLR
                     HWGDQVSQL"
BASE COUNT          594 a          854 c          910 g          556 t
ORIGIN      
        1 ggtctccgga aacaggaact ggagcaggcc cgcatcgaat tcgaacgctg gcaggacgaa
       61 tacaagcgca tgaagttcct ggtggaacga aaaagcctgc ctccgaacga ttttcagaaa
      121 atcgaagcgg cctataacgc ctcgaaagag cgttacgaga tggctgtaga gggcacgcgc
      181 aaggaagaca tcgccacggc ggctgcacag tcacatgcgg cagacgccca ggcggccgag
      241 gaacgaaagc ggttggacga cacgcggctt ctcgcgccga tcttcggaaa tgtatcaatg
      301 cgcagggttg accccggcca gacagtcgcc gccggaatgc cggtcttctc gatcgtcgaa
      361 ctgaacccgg tcaaggttcg cgtgggagtt cccgaagcgg agatcgccaa agtccgccaa
      421 ggcgccgccg cggagatcac cgtgccttcg ctcgacggac gatgcttcaa aggaaaagtg
      481 gccattatcg gcgtcgcgag cgaacccacc tcacgcacct atacggtaaa aatcctcgtt
      541 cccaaccctg gaccggtgct gctggccggt atggttgcgg aagcccggat cttcgggcct
      601 gcgaagctcc gcccgctgac catcccggct gaggccgtgg tgcccgatgc acagggcgcg
      661 ccaaacgtgt atatctactc cccggaccgt aagcgggtct acgggcgcag ggtcgaggtg
      721 ggcacgccta ttggtaatga agtcgaaatc cactccggcc tgcaagggga cgagcagatt
      781 gtcgtcgtgg gccagcagaa ggtccgtgaa ggcagcctgg tggagatcgc gggaggcgcc
      841 cgatgaatcc cgttcgcgca tcgctgcgct acccgcaagt cacccttgca ctcacgatca
      901 tgttggtcgc ctccggggtg tatgctttgc tgcgtatgcc gcgacgggag gatccgaaga
      961 ttcacgtccg catcggaatc gtggctgcct tctatccggg cgcatcggcc gcggaggtcg
     1021 agaatcaggt cactcagaaa gtcgagcagc gtctcttcaa aatcgagggt gtgcggcggg
     1081 aaaagacgta ctcgacaagc atgaacggag tcatgtttat caacctgaac ctggaagaca
     1141 accttcagga cacggagaag ttctggtcca agctgcgtct cgacatggcg gagctgaaga
     1201 agacggatct tccggatggc gtggaaggcc ctatcgtcga ttacgacttc ggcgacacgg
     1261 tggcggtact attggcgatt catggcgacc actacggcta ccgcgagctg aaagattatg
     1321 ccgagcggat cgagtcgtca atccgccgca ttcggtccgt ctccaaggtc aagcgcatcg
     1381 gcgagcagaa ggaggagatt gacgtatcga gttccctcag ccgcctgtcg caatactccg
     1441 taacaccgga gaaggtcttt aaggcgctcg aaggccgcaa catggtgcac tatgccggag
     1501 cggtgccgac cgaacacgga aaaccgccca ttcagacgac gggcgcattc gagggtgagg
     1561 agcagatccg gcgcgtgatg atcgatgtca cgcgcacggg acagccggtc tacatcggcg
     1621 acctggcgaa tgtcgaccgc gtttacaagg acccgacgca atatgcgcga tcaagaggcg
     1681 agcgggcgat gctgctctcg gtggagatgc aggaaggcaa caacatcgtg gacttcggca
     1741 aggagcttca cgcttccctg gatgccctgc gcccgctgtt gccacccgac ctgaagattg
     1801 atttcgttgc cgaccagccc acagtcgtcg ccgaacgcgt gaagagtttt atccgcgagt
     1861 ttgggatctc catcgcctcg gtgatcctgg tcacgatgtt gctgctgccg ttccgtgtgg
     1921 cgctggtgtc cgccgtggcg attccggtga ccatttcggc gaccttcggc gtgctgaacg
     1981 ctttcggcat cgaactgcac caggtgtcga tcgccgcatt gattgtggtg cttgggatgg
     2041 tggtggacga tgccatcgtg atcgcggata actacatcga gctgctcgat cacggcatag
     2101 ctcgagagga ggccgcatgg cgcagcgcga ccgagcttgc gatacccgtg cttaccgcca
     2161 ccctgaccat catctgttcg ttcctgccct tccttctgct ttcgggcgcc accggagagt
     2221 tcatccgcgc gctgcccttg acggtggcga tcgcgctcgc gacatcgttc ctcgtggcca
     2281 tgctgctgac tcccatgttg agtcagtttt tcattcgcaa gggcctccac aatcccgaag
     2341 acagctcggc caagaagcgg cgcgggcctc tcgaaatcat gcaggcgatc tacaaccgcg
     2401 tcattgtagt cgccatgcgg tggaaggttg ccgcggtcgc attcggcgta ttcgctgtgg
     2461 cggcgggcgc attcatgctc catatcgttc cggagcgttt ctttccgttc gccgagcgcg
     2521 accagttcgt gatcgacgtc tggctcccgg aaggctggaa ggtggaagcc acggagtccg
     2581 cggtgaagcg catcgaggac gtgctgagtc acgagaagga agttgtcaat ttcacctcgt
     2641 ttatcgggtc gagctttccg cgcttctact acaatgtcaa tccgcagttg ccggacaaga
     2701 actacgcaca gatgcttgtc tcgaccaagt cggcggaagt cacaccggag ctggtaagag
     2761 cgctgcgccc acaactcgcg caggccgctc cggaagcacg ggtgttcctc agcgaactgc
     2821 agcaaggtcc ggtccaggag gccgccatcg aggttcgcct tacaggcgat gaccaggccg
     2881 tgctgcggca ttggggcgat caggtgagcc agct
//
LOCUS       DATMGX010000351         2564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_155192, whole genome shotgun sequence.
ACCESSION   DATMGX010000351 DATMGX010000000
VERSION     DATMGX010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155192"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VNR65_05615"
     CDS             <1..857
                     /locus_tag="VNR65_05615"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HWJ18190.1"
                     /translation="AISQVKMIELKLSQGAKPGKGGILPAIKVTPEIAQIRGIPVHTD
                     SISPNRHAEIGSVGDLIDCIGRIRAIAGKPVGFKSVIGAYGWLEAMCEEIHMRGLESA
                     PDFITVDSGDGGTGAAPMALMDNVGLTIRESLPLVVDLLRRHGLRDRIKVFASGKLIS
                     PAEVAWALCAGADSVNSARGFMFALGCIQSLKCNKNTCPTGITTHDRRLQHGLNPAEK
                     AVRVANYAAKLEKEVGVIAHSCGVSNPRALRRYHCRIVQADGRSIPMNELYPDIVKAD
                     RAIARATA"
     gene            898..1995
                     /locus_tag="VNR65_05620"
     CDS             898..1995
                     /locus_tag="VNR65_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18191.1"
                     /translation="MTHCPVYKKGSSEETPFALQRRELLIGAGIAGFLAAGTGQASAQ
                     MGAKTAGAAAPSVVAPMADMIPAGYDPAYVEHVIMPFLRTSFYQAETPSLPMIGEAFS
                     KQYAIPYDLWGLLYDDWAPSFERDGLSVFILGLDKRGPDNRRKRIYFSALTADLYPMY
                     AGKVRAFFDKLLDEKNAGKPLMRRYLDSYFDLFWDLHLGVTGDAIPADVRQIGHSFNT
                     VLGFRDPLLEIVYDNYMKVRALRTPLKQWIDEKTNDIMSGKIKDPEKTFVYYWTKNGG
                     KGEDFRQKDIVFECFHNFAALSQWGNTIYNIMLKLDKNAGRSGGQVLVQEDDGRRSRQ
                     GGPRRLHAASASRHGAFPDDFAQSREHFHPS"
     gene            1940..2494
                     /locus_tag="VNR65_05625"
     CDS             1940..2494
                     /locus_tag="VNR65_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18192.1"
                     /translation="MELFRTISPNPGSISTLHELKPVHGADRYVYAITPHAPASFDAS
                     QWKNPLEFNPDRYLNSPTSAENSETKARALGFAKCPFEKSSFPVSDGRKAEIVNSAFG
                     TVYGVVEGKPLPVCDHTGYAPFGFGYRRCPGEQLNIEVFSDFLRKVWADKIEFEQLAT
                     PNPAKIPIGPVTVIDDAIGFRRRA"
BASE COUNT          566 a          758 c          741 g          499 t
ORIGIN      
        1 cggccatcag ccaagttaaa atgatcgagt tgaagctgag ccagggagca aagccgggaa
       61 aaggcgggat cctgcccgcc ataaaagtca cacctgaaat cgcgcaaatc cgaggaattc
      121 ctgttcacac cgattccatc tcacccaatc gccacgccga aatcggcagc gtcggcgatc
      181 ttatcgactg catcggccgg atacgcgcca ttgccggaaa gcccgtcggc ttcaagtccg
      241 tcattggcgc ctatgggtgg ctggaagcga tgtgcgagga aatccacatg cgcgggctgg
      301 agagcgcgcc cgacttcatc accgtcgatt ccggcgatgg cggaacaggg gcagcgccca
      361 tggcgctgat ggacaatgtc gggctcacca tcagggagtc gcttcccctc gtcgtcgatc
      421 ttttgcgccg tcacggcttg agggatcgga tcaaggtttt cgcatccggc aaattgattt
      481 cgcccgccga agtcgcctgg gcgctttgcg caggcgccga tagcgtgaac tcggcgcgcg
      541 gcttcatgtt cgcgcttggc tgcatccaat cgctgaaatg caacaagaac acatgcccga
      601 ccggcatcac aacgcatgat cgccggctcc agcacgggct aaacccagca gagaaagcgg
      661 tgcgcgtcgc aaattatgca gcgaagcttg agaaggaagt tggcgtcatc gcgcattcct
      721 gcggcgtatc caaccctcgc gcattgcgcc gttatcattg ccgcatcgtt caggcggacg
      781 gccgctccat tccgatgaac gagctttatc cagacattgt gaaggctgat cgcgctatcg
      841 ctcgcgcgac agcataaagg aacttgccga attaagagtt ggcgcgggga ggaaaccatg
      901 acgcactgcc cagtttataa gaaaggaagt tcagaagaga cgccctttgc cctacagcgg
      961 cgcgagcttc tgatcggcgc cgggattgca gggtttctcg ccgccggaac gggtcaagcc
     1021 agcgctcaga tgggcgccaa gacagccggc gcggcggccc cttcggtcgt agctcctatg
     1081 gcggatatga tccctgcggg ctacgacccc gcatatgtcg agcatgtcat catgcccttc
     1141 ctgcgcacga gcttctatca ggccgaaact ccgtcccttc ccatgatcgg cgaagctttc
     1201 agcaagcagt acgcaattcc ctacgatctt tggggtttgc tgtatgacga ttgggcgccg
     1261 agcttcgaga gggatgggct ctcggttttt atcctgggcc tcgataagcg cggaccggat
     1321 aatcggcgca agcgcatcta cttctccgcg ctgacggccg atctctatcc catgtacgcc
     1381 ggcaaggtcc gagcgttttt cgataagctc ctcgacgaga agaacgccgg caagccactg
     1441 atgcggcgtt atctcgacag ctatttcgac ctgttctggg atctgcatct gggcgtgacg
     1501 ggcgatgcaa ttccggctga cgtccgccag atcggccaca gcttcaacac ggttctgggc
     1561 ttccgagatc ccttgctcga aattgtctac gacaattaca tgaaagtgcg ggcgctccgg
     1621 acgccgctga agcagtggat cgacgagaaa accaacgaca tcatgagcgg gaagataaag
     1681 gaccccgaaa agaccttcgt ttattactgg acgaagaacg gcggcaaggg cgaggatttc
     1741 aggcaaaagg acatcgtctt cgagtgcttc cacaattttg cggccctcag ccagtggggc
     1801 aacacgattt acaacatcat gctgaagctc gacaagaacg cgggccgatc cggcggtcaa
     1861 gtcctggttc aagaagacga tggaaggcgg tcccgacaag gagggccccg gcgccttcac
     1921 gccgcttcag cgtctcgtca tggagctttt ccggacgatt tcgcccaatc cagggagcat
     1981 ttccaccctt catgaactga aacccgtcca cggcgccgac cgctatgtct acgcgatcac
     2041 gccgcatgcg ccggcgagct tcgatgcgag ccaatggaag aatccgctgg agttcaatcc
     2101 ggaccgctat ctcaactcgc ccaccagcgc tgagaatagc gagacgaaag ccagggcgct
     2161 tggatttgcg aaatgcccgt tcgaaaaaag ctcatttccg gtcagcgacg gcagaaaggc
     2221 cgagattgtc aacagcgcct tcgggaccgt ttatggtgtg gtcgaaggca agccgcttcc
     2281 cgtctgcgac cacacaggct atgcgccttt cggcttcggc taccggcgct gccccggcga
     2341 gcagctcaac atcgaggttt tctcggactt cttgaggaaa gtctgggcgg ataaaattga
     2401 gttcgagcaa ctcgctacgc caaacccggc gaagatcccg atcgggccgg tcaccgtcat
     2461 cgatgacgcc atcggattca ggcgtcgagc ctagctgctg aacgaaaagc aaaagggatc
     2521 tgtcaatgga tatgagcgtt cgcagcggtc tcgccgatat cccg
//
LOCUS       DATMGX010000352         4395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_318508, whole genome shotgun sequence.
ACCESSION   DATMGX010000352 DATMGX010000000
VERSION     DATMGX010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318508"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            579..956
                     /locus_tag="VNR65_05630"
     CDS             579..956
                     /locus_tag="VNR65_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18193.1"
                     /translation="MSQHRRSQRLALAAAIFMPMAATIPAFAADTVVSSHEQAQQLRS
                     GERHQVYAYEKQTFICGETTDKEGKRRSLVGEPYEPRLPMWEPKKDDPIWTTWDITFK
                     IICKNFDQQPNIDTASGDQRSVR"
     gene            2737..3909
                     /locus_tag="VNR65_05635"
     CDS             2737..3909
                     /locus_tag="VNR65_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18194.1"
                     /translation="MAIALVLGGGAPNLTLMAGAVAALDDAGVKFDIVSTSGAGMLIG
                     LLYAAPKGVSRAVALKNTVNMGVHDAIYNLFPVNFKVFHKPGALAQAYTKFWQLAADN
                     YKATEVSAGDFRDQWLKMLSASPWLAPWAKEWTQFMGKFLSPPHKIGDAQRFFDDWAA
                     LMLATSCPSDLSSTSPGMCQPAPFVEQVVEFSRLREFDGEFYMSAYCIETESMEIFEK
                     KDVSLEHFQAALAFPLIYAPFKMNGKTYIEGAAKDTLNFDGLIKHRKGHAKVIDTIVV
                     LDVLGIDELITEPRSLYDAWVKSIVVPLTAIAKDDIKLFEEIHLKDQKNLDIYFGGNK
                     PKVVKIDFKTQLNDMKYNWPNMLDWSYSNLSTLYEAGYRAGRYAAKDKHLATYKTK"
     gene            complement(4125..>4395)
                     /locus_tag="VNR65_05640"
     CDS             complement(4125..>4395)
                     /locus_tag="VNR65_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18195.1"
                     /translation="HRRRGGVLLNGTARIVEAQACFENAIAIARRRQTRSWELRAACD
                     LAGLMAEQGKRGEAHNLLAGVYGWFTEGLDTPDLKVAKALIEKLR"
BASE COUNT         1041 a         1251 c         1142 g          961 t
ORIGIN      
        1 cccgggcttt gggcctgttc gcgcttctct acggccagac cccgttgaaa agctcgggga
       61 aaccctcaat ggccagcgcg cgttgcggca acgccggctc ctctcgttga gccgtcacta
      121 tcttcgcgag cgctgcgcac gtcgccataa gcaaaatggc ggctgacagt gccaagaacg
      181 ttttagacag acgtgtcatg ctgatgtcct cgggttgggc agtcgatccc attcgacctg
      241 ctcccaaagt aggccatcgc ttcgccgagg gctgttcctt ccgaaacagc gtccaagttt
      301 tctggcgcgt tcgttgcagc cttcgtcggc cggtagcgcc gatcgccgcc ggctctcact
      361 cccccgccac aaccaccccc tccaaccgcg gcttcgaatt atcgccgcgc gtatgctcgc
      421 cgaaatggtg atgcggaacg cgctgcgccg attgcaatgc gagcaccgca gccgaaatta
      481 aaaattcttt tagttgttcc gtacgtaaca ccgtcacccc cgagcttgtg gaatgatgtc
      541 cctcgaaagc aggccgctaa cattcgaaag gatatactat gtcacagcat cgccgctcgc
      601 agcgtctggc tcttgccgct gcaatcttta tgcccatggc cgctaccatc cctgctttcg
      661 ccgcagatac cgtcgtgtca agccacgaac aagcccagca gcttcgcagt ggcgagaggc
      721 atcaagtgta tgcatatgag aagcaaacct ttatttgcgg agagacgaca gacaaagaag
      781 gcaaacgtcg tagccttgta ggcgaaccat atgaaccgcg acttcctatg tgggaaccca
      841 agaaagatga ccctatttgg acgacttggg atattacctt taagatcata tgcaagaatt
      901 ttgatcagca gcccaacatc gacactgctt cgggcgacca acgctctgtt cgctagcgga
      961 ccatcgctta ttaaacctca acacggcgga gctttcgcca tctcttatcc tcgaataacg
     1021 gcgcgactcg gctagctctc gccagcgttc aatcgtcctg gcttggggct ttggcaacta
     1081 ccgcacgcgg taagtattga aagaccgcag gctcagcacg cagtatgaat ggaaatgcgc
     1141 tgcaagagcg ccgttcgccc cccttcgttg cttctctggc tcttgcgctt gcacttacgt
     1201 gagatcagac tatcactaaa agttgaagac catcttgcga ttcctcgtga agtctaccaa
     1261 gagctcatcc tggtgcatcg tttattgtat tgtcaaatat agaaaatcca caaagtttgc
     1321 tgctgaactc acattagaaa acaattacgt tctctgctcc ggcgtagcaa agataaacac
     1381 tacgaaataa ttcgacattg ttacctgagg aacagcccat agcgaagcga tggactacta
     1441 tttttggaga agatcgaata tatcgacttc ttaacagagg agctcatcat gtctgtcaat
     1501 cctttcaaaa agtttttcac gttttcagcg agtatgttgc ttgtggcagc gtccgtaacg
     1561 tcgtcggatg caagaccgga tgcaaatcgg catgagccga ctcaagttga atccccgcgt
     1621 agttcggata attgcacggt atggggacac cacatctatc cctgtgagtg acctcaagcc
     1681 accccggctc aatattccgg ggctcccgct atattcgcgt gcagcggcaa ttaaaataaa
     1741 tagtttcgaa aacaggagaa taactatgaa gaccatcttc acaatatcgg gcggcgtttt
     1801 actggcgcta tcctgcgtga cagcgtccga tgctagacag tatgccaaac ggcaaactcc
     1861 aactcaaagc gagtcctcct acgatcgctc ggattacgat accacacgcg accgggatag
     1921 ttcctgcttt agtcgttcta ccggcctttc tgatcaatat gcatgcagcc acggcggcgg
     1981 atgagcgccg cactgaaccg cgcctctaag gcgcggttct ttaaaaccga tccggaaaat
     2041 ggcgcgcgcg acgaacgcga gcacttggtc gccaaccctg gcgtgcatcg cccggaggag
     2101 aataactatg aagacctttg ttgcaatatc gggcggtatt ttgctgacgc tatcctgtgt
     2161 agcaacatcg gatgcaagac cgcacgccaa ccaggatggt ccaactcaaa gcgagccctc
     2221 ccactacaat ccggattacg atagccaaag aaagacgcgg aactgggata attcctgcgt
     2281 tcattccgtt cctgctatgt actggtgtaa cagttcggat ttgccaagcc gcagttcccg
     2341 gtagaagaaa gtcattgcac aactggtgct agagtaatgc gcatgcaaac taccatccga
     2401 ttagggccgg gccgcgagat aaccatcccc gcttgtcccc agcaacggac gagctgatca
     2461 aaaagtagaa ggtccgcccc cggtcttgac cgcggcggcc gaaaataccg tagttttact
     2521 gtgacaaccc cgcaatacta tagtataaat gatccatgat cggctctaaa cgggtcttat
     2581 gccatgatat cgaaaagttt atcgcgcttc gtgcttggcg atcatgactc tggcatcgaa
     2641 agtgcgtcgg cggtattggc gatcgccgac tagcctgcgc gcgtcaagaa ggcttgggcg
     2701 caacgcgcgc aggctgctca tgtgggaggg ataagcatgg caatcgcact ggtgctgggc
     2761 ggtggcgcgc cgaatctgac gctgatggca ggcgctgtgg ccgcgctcga cgatgcgggc
     2821 gtgaaattcg acatcgtctc gacttcgggc gcgggcatgt tgattggcct gctctacgca
     2881 gcacccaagg gcgtgagccg cgcggtagcg ctcaagaaca ccgtcaacat gggcgtgcac
     2941 gacgctatct ataacctctt cccggtgaat tttaaggtct ttcacaaacc cggcgcgctc
     3001 gcacaagcgt acaccaaatt ctggcagctt gcggcggaca actacaaggc gaccgaggtg
     3061 tcggccggcg attttcggga ccaatggctc aaaatgctat cggcatcgcc gtggcttgcg
     3121 ccctgggcaa aagagtggac gcagttcatg ggcaagtttt tgtcgccgcc ccacaagatc
     3181 ggcgacgccc agcgcttttt cgacgactgg gccgctctga tgttggcaac ctcttgcccg
     3241 agtgacctca gttcgacaag ccctggcatg tgccagccgg cgccgttcgt agaacaggtt
     3301 gtagagttct cccggctgcg cgagttcgac ggcgaattct atatgagcgc ctattgcatt
     3361 gaaacggaaa gtatggagat tttcgaaaag aaagacgtca gtttagagca tttccaagcg
     3421 gcgcttgcat ttcctctaat ttatgcgccg ttcaagatga acggcaagac ctacatcgag
     3481 ggcgcggcca aggacaccct caatttcgat ggcctaatta agcaccgcaa gggccacgcc
     3541 aaagttattg ataccatcgt tgtactcgat gttctgggca tagacgaact aatcacagaa
     3601 ccgcgcagtc tctacgacgc atgggtgaag tcgatcgtcg tgccgctgac cgcgattgcc
     3661 aaagacgaca tcaaactgtt cgaggagatt cacctgaaag atcagaaaaa cctagatata
     3721 tatttcggcg gcaataaacc taaagtcgtc aagatcgact ttaaaacaca acttaatgat
     3781 atgaaataca attggccgaa tatgctcgat tggtcctaca gcaatctgtc aacgctgtac
     3841 gaagccggat accgcgcagg tcgttatgct gccaaagaca agcatttagc cacatacaaa
     3901 acgaaatagc cgccgggcgt tagcgaaagc ccttggctta gacagccctc caaactaaca
     3961 ttgtgtggga aagttccgat gactggccct tatccctcgc caagcatctc ctcgaccgct
     4021 gatgctgtgc ttcacccaag cttctcgagg tagtcacgtc ggcgtcacat tacgtcgcct
     4081 cgctgcgctc aagtccaaat gcctgaacca gaccgcgaca tcgctcaccg cagcttctct
     4141 atcagcgctt tggcaacttt gaggtccggc gtgtcgaggc cttcggtgaa ccagccgtat
     4201 acgcccgcga ggaggttgtg cgcctcgccg cgcttgccct gctccgccat cagaccggcc
     4261 aagtcgcagg cagcgcggag ctcccaggaa cgagtctgcc ggcggcgggc aatggcgatc
     4321 gcgttctcga agcaagcctg ggcttcgacg atgcgtgcgg ttccgttcag cagaacgccg
     4381 ccgcggcggc ggtgg
//
LOCUS       DATMGX010000353         2594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_332085, whole genome shotgun sequence.
ACCESSION   DATMGX010000353 DATMGX010000000
VERSION     DATMGX010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332085"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(658..1713)
                     /locus_tag="VNR65_05645"
     CDS             complement(658..1713)
                     /locus_tag="VNR65_05645"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ18196.1"
                     /translation="MYISHYIGLDVHKKSISYCVKTADGQILEEGKLRATHQVLREWA
                     QKRSEPWHGAMEATLFSGWIYDALKPFAAELQMGNPSMMKAIGAAKKKSDQLDARKIA
                     DLVRCNLLPVCYVASAEMRDLRRMLRYRNLVVGQAVQMKNRMSGMLMEVGAEYRKQRL
                     HGKQYFSELLDQLEEVPASVKDLLRLSRSALDVFETTQRQLLDRLQKEPLLVKRVKLL
                     RSIPGVGEVTALTWALETCDPRRFPSIGDAVSYCGLVSALVSSADKAKRGPISKQRNA
                     HLQTVLIEAAKLAPRWNQPLAELHERELKRGDRNQATLAVARKLVAYLLAVDKSGKRF
                     EVRKPPVREMEEVGQMA"
     gene            complement(1922..>2594)
                     /locus_tag="VNR65_05650"
     CDS             complement(1922..>2594)
                     /locus_tag="VNR65_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HWJ18197.1"
                     /translation="ITVDKDNTTIVDGAGESKKITGRINQLRAQIEETTIDYDREKLQ
                     ERLAKLAGGVAVIKVGAATETEMKEKKARVEDALHATRAAVEEGIVPGGGVALLRAAQ
                     ALAGFKVDGDEQIGVTIVRRACEEPIRQIVSNSGTEGAIVVDKVRTNANPNFGYNAAT
                     DTYEDLVSTGVIDPTKVTRSALQHAASIAGLMLTTEAMIAEIPEKKSAPAGGPGGHGG
                     GEMDY"
BASE COUNT          441 a          844 c          749 g          560 t
ORIGIN      
        1 tatgggccgt cggcggacga ggcaagctag gtttctccct gcagtacgat tgggggcgga
       61 cttcggtccg ccctcttttt tgagtggcat aagttgctca tcgtgccagg ggcgacacct
      121 gtggggcatc catgataagt acttgtcaag ggaaaaaaca cttctccctg cactccccag
      181 aaggatttga agcgcctgga taatgaagcc gaccgttagc gcgacgattg accgtttgcg
      241 atccgttgag gttggggtca acgcatcagc gggaccagac atccatttgc gggaacagca
      301 tagcaccgtc cacgaagttt gaatttgggt aagccggccg gcctcgaaac agccaagatg
      361 gcacatcggg aggaaaacct ccgtcgtgca gccccacaaa cgcgaggaac ggccggtgaa
      421 tcgctgatca atcgttgcat tgctacacac cgcggggtct tggcgatttt cccgccggag
      481 ccgcccgtcg catcgatgct tccggcggaa cggcccggag cggtcaaggg cgcgcccgct
      541 tttggcgcgg cggagcgaac ccttgacggc gaggaccgtt ctggcatcgg acccatcacg
      601 acgggcggct ttggtgggaa aatcaccttt cccgctggtg aggctcagag cgggttttta
      661 agccatttgc cctacctcct ccatttccct caccggcggc ttgcgcacct cgaagcgttt
      721 gcccgatttg tccaccgcca acagatacgc taccagcttc cgcgccactg ccaaggtcgc
      781 ctggttgcga tcgccgcgct tcagctcccg ttcgtggagt tcggccaacg gttggttcca
      841 ccgcggcgcc agtttcgccg cctcgatcag caccgtctgc aaatgcgcgt tgcgctgctt
      901 ggagatcggc ccgcgtttgg ctttgtccgc cgacgatacc agcgccgata ccagaccgca
      961 gtagctgact gcatccccaa tggacgggaa ccgccgcggg tcgcaggtct ccagcgccca
     1021 ggttagcgcc gttacctcgc ccacccctgg tatgctcctc agcagcttca cccgtttcac
     1081 cagcaatggc tctttctgca atcggtcgag caattgccgt tgcgtcgtct cgaacacgtc
     1141 cagcgcactc cggctcagcc gcagcagatc cttcaccgat gccggcacct cctccaattg
     1201 gtccagcaat tcgctgaaat actgcttgcc gtgcaggcgt tgtttgcggt attccgctcc
     1261 cacctccatc agcattccgc tcattcggtt cttcatctgc accgcctgcc ccaccaccag
     1321 gttccggtag cgcagcatcc gccgcaaatc gcgcatctcc gccgatgcca catagcagac
     1381 cggcagcagg ttgcagcgca ccaggtcggc aatcttccgc gcatccagtt ggtcgctctt
     1441 cttcttggcc gccccgatgg ccttcatcat cgaggggttt cccatctgca gttcggcggc
     1501 aaacggcttc agcgcatcgt aaatccaccc gctgaacagt gtggcttcca tcgccccatg
     1561 ccacggctct gagcgcttct gtgcccactc ccgcaacact tggtgcgttg cccgcagttt
     1621 gccttcctcg aggatctgtc cgtcggcggt cttcacgcag tagctgatgc ttttcttgtg
     1681 aacgtccaat ccgatatagt gactgatgta cattgggttc tcctgtttga ggtttctgag
     1741 ccagttcgca aaaaccggtc tcggcattgt acccttcggg ggaacccaac atacttatac
     1801 attcaaacag ccaatcctgg ctgcagccgg cgcctttcgg caagcggcgc aaaccattaa
     1861 aaaagccgcg ccccccgatg aggggagcgc ggccaggttt cactgaaaaa aggaaggatt
     1921 cttagtaatc catctcgccg ccgccgtgtc cgccgggacc gccggccgga gccgacttct
     1981 tctcggggat ctcggcgatc atggcttcgg tggtcagcat caggccggcg atggacgccg
     2041 cgtgctgcag ggccgagcgg gtcaccttgg tcggatcgat gacgccggtc gataccaggt
     2101 cttcgtaggt atcggtggcg gcattgtagc cgaagttggg gttcgcgttg gtgcggacct
     2161 tgtccaccac gatggcgcct tcggtgccgg agttggagac gatctggcga atcggctctt
     2221 cgcaggcgcg cctcacgatg gtgacgccga tctgctcgtc gccatccacc ttgaagccgg
     2281 ccagggcctg ggctgcgcgc aacagcgcga cgccgccgcc gggaacgatg ccttcctcaa
     2341 ccgccgcgcg ggtggcatgc agggcatctt ccacgcgggc cttcttttcc ttcatttcgg
     2401 tctcggtggc cgcgccgacc ttgattaccg cgacgccgcc ggcgagcttc gccaggcgct
     2461 cctggagttt ctcgcggtcg taatcgatgg tggtctcctc gatctgcgcg cggagctgat
     2521 tgatacgccc cgtgattttc ttcgactcgc cggcgccgtc cacgatggtg gtgttgtcct
     2581 tgtccaccgt gatg
//
LOCUS       DATMGX010000354         3179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_383734, whole genome shotgun sequence.
ACCESSION   DATMGX010000354 DATMGX010000000
VERSION     DATMGX010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383734"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /gene="gatB"
                     /locus_tag="VNR65_05655"
     CDS             <1..1090
                     /gene="gatB"
                     /locus_tag="VNR65_05655"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWJ18198.1"
                     /translation="GITRVHLEEDAGKSLHDGLPDSGDWTSIDLNRSGVPLIEIVSEP
                     DIASPDEAHEYLTRLKEIILYTGVSDCNMEEGSLRCDANVSIRPRGETKFGTKAEVKN
                     VNSFRFVRHALEYEIERHQQVLESGGKIVQETRLYNPAEGKTYSMRSKEQAHDYRYFP
                     EPDLLPLVVDEKWRKQIMDQLPELPAARRARMVVQYGITEYDATTLTGTRSLADQFEE
                     AAKAAKNPKRVSNLVQSELLGRLKAAGLEIEQSPISMKGVAMSADLVESGAISGKMLK
                     DLYDLAFERGEDFPAVFEKEKPQQLTDTSAIEKIIDEVIAGNPRQLEQYRAGKKTVIG
                     FFVGQVMKASKGQANPALVNELLAKKLE"
     gene            1279..2340
                     /locus_tag="VNR65_05660"
     CDS             1279..2340
                     /locus_tag="VNR65_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18199.1"
                     /translation="MIAALSGCGTPGAPQPPSLQLARPVDDLTASRKGNRVELDWTLQ
                     RKNTDRTLVKRLPAIRICRREGTGLMSACAVVAEVPPPKPQLQPKRKGKEESPAVRMH
                     YLDSLPPQLGRENPVDFVTYAVEEINSHGRSAGLSNQVPIPVAPTIPAPNELSAQVSA
                     EGVRISWSGPAPPAAIAGLSYRYRIMRRPVGAPAYTVLADVEPAATGSYLDKTFAWEQ
                     KYEYRITTLTQVDSHGINTAVEGDDSQPAEVFTRDIYPPAQPAGLQAVFSSVGQKPFI
                     DLTWAPNLESDLAGYNIFRRVGNAQFEKLNKQLVSGSSFRDDNVETGRKYIYSVSAVD
                     LRGNESQRSAEASETVPGK"
     gene            2358..3152
                     /locus_tag="VNR65_05665"
     CDS             2358..3152
                     /locus_tag="VNR65_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWJ18200.1"
                     /translation="MFYCRFQYQNFNRYGLIENVRGVETITRTRATAPRSWSDFEGAD
                     RADLPLSEARLLSPAEPSKVVCIGRNYLEHAKELNHPIPTEPLIFLKPPSSVIATGDA
                     IRRPNKLSQRVDYEGELGVVIGKRCYRLREGDDVRPYVLGYTCVNDVTARDLQEKDGQ
                     WTRAKGFDSFCPVGPVVAKDLDPWKGVSVQTRVNGQLRQNGTTVDFLFPLDVLIHFIS
                     QVMTLEAGDLIATGTPVGVGPLQPGDSVEVAVEGVGTLKNPVLNLE"
BASE COUNT          669 a          960 c          946 g          604 t
ORIGIN      
        1 cggcatcacc cgcgtgcacc tggaagaaga cgccggcaag agcctccacg atggccttcc
       61 cgacagcggc gactggacct cgattgacct gaatcgctcc ggggtgccgc tgatcgagat
      121 cgtcagcgag cccgatatcg cgtcgcccga cgaagcccat gaatacctga cgcggttgaa
      181 ggagatcatc ctctacaccg gcgtcagcga ctgcaacatg gaagaaggct cgctgcgttg
      241 cgacgccaat gtcagcatcc ggccgcgcgg cgaaaccaag ttcggcacca aggcggaagt
      301 gaagaacgtc aactccttcc gttttgttcg ccacgcgctg gaatacgaga tcgagcgcca
      361 ccagcaggtg ctcgagtccg gcggcaagat tgtgcaggag acgcgcctct acaaccccgc
      421 tgagggcaag acctatagca tgcgctccaa ggagcaggcg cacgattacc gctatttccc
      481 tgagcccgac ttgctgccgc tggtggtgga cgagaagtgg cgcaaacaga tcatggacca
      541 gttgcccgaa ctgcccgcgg cccggcgcgc gcgcatggtt gtgcaatatg gcatcaccga
      601 atacgacgcg accacgctga ccggcactcg ctcgctggcc gatcagtttg aagaggctgc
      661 gaaagccgca aagaatccca agcgcgtctc caacctggtg cagagcgagt tgcttggccg
      721 gttgaaagcc gcggggctgg aaatcgagca atcgccgatt tcgatgaagg gcgttgccat
      781 gtcggccgac ttggtcgaga gcggcgccat cagcggcaag atgctaaaag acctttacga
      841 tctggctttt gagcgcggcg aggacttccc cgcggtattc gagaaggaga agccacagca
      901 gcttaccgat acctcagcca tcgagaagat cattgacgag gtgatcgccg gcaatcccag
      961 gcaactggag cagtaccgcg caggcaagaa gaccgtgatc ggcttcttcg tcggtcaggt
     1021 catgaaggca tccaagggac aggccaatcc ggcgttggtc aacgaacttc tggccaagaa
     1081 gctggaatag tgagccacaa agacacgaat agcggtgtca accctgttag cttgattatt
     1141 tgaatatcaa gttatttaac ttgcttctcg agattcaaac ttacggaatt ggtaaccccc
     1201 ggattgaact gcgcaggatt tgttaccctg ttagcttcca tgagaatgcg cctctattgt
     1261 ctggccggaa gtttgcttgt gatcgcggcg ctgtccggct gtggaactcc cggagcgccg
     1321 caaccgccgt cgttgcaact ggcgcgcccg gttgacgact tgaccgcctc ccgcaagggc
     1381 aaccgcgtcg aactcgactg gactctgcaa cgtaagaata ccgaccggac cctggtcaag
     1441 cgcctgcccg caatccgcat ctgccgtcgc gaaggcactg ggttaatgag cgcctgtgcc
     1501 gtggtggccg aagttcctcc acctaagccc cagcttcagc cgaaacggaa ggggaaggag
     1561 gagtcgccgg cggttcggat gcattacttg gattcgctgc cgccacagtt ggggcgggag
     1621 aatcccgtgg acttcgtcac ctatgcggtg gaagagatca attctcacgg gcgcagtgcc
     1681 gggctctcca atcaggtgcc gattccggtg gctccgacca tccctgcccc caacgaattg
     1741 tcggcccagg tcagcgcaga aggcgtgcgc atctcgtgga gcggccccgc tccacccgcg
     1801 gccatcgctg gtctcagcta ccgctaccgc atcatgcgaa ggccagttgg cgctcccgcg
     1861 tataccgtat tggccgatgt agagcctgcg gccaccggat cgtatcttga caagactttc
     1921 gcgtgggagc agaagtacga atatcgcatc acgaccctca cgcaggtcga ttcgcatggc
     1981 atcaacaccg cggtcgaggg cgatgactcg cagccggccg aggtgttcac gcgtgacatt
     2041 tatccgccgg cccagcccgc cggactgcag gcggttttct ccagcgtggg ccagaagcca
     2101 ttcatcgacc tcacctgggc cccgaatttg gagagcgatc ttgcggggta caacatcttt
     2161 cggcgcgtcg ggaacgcgca gtttgaaaag ctgaacaagc aattggtttc gggctcttca
     2221 ttccgtgacg acaatgtcga gaccggcagg aaatatattt attccgtttc cgccgtggat
     2281 ctgcggggca atgagagcca acgctccgcc gaggccagtg aaacagtccc tggcaagtaa
     2341 gcattcatcg aggttccatg ttttactgcc gatttcaata ccagaatttc aaccgctatg
     2401 gtctcatcga gaacgtgcgc ggcgtcgaga cgatcactcg cacaagggcg accgctccgc
     2461 gttcctggtc cgactttgag ggcgctgacc gcgccgatct gcccctgagc gaagcgcgct
     2521 tgctgtctcc ggcggagccg tcgaaagtgg tttgcatagg ccgcaattat ctcgaacacg
     2581 cgaaagagtt gaaccatcca attcccacgg agccgttgat ctttctcaag ccgccgtcgt
     2641 ccgtcatcgc caccggcgac gccatccgcc ggcccaacaa gctctcgcaa cgcgtggact
     2701 acgaaggcga gctgggagtc gtgatcggca agcgctgcta ccgcttgcgc gaaggggatg
     2761 acgtgcgccc ttacgtactg ggctatacgt gcgtgaacga cgtgactgcg cgtgacctgc
     2821 aggagaaaga tggccagtgg actcgggcca agggattcga ctccttttgt cctgtcggcc
     2881 cggtggtggc gaaggacctc gatccctgga agggcgtaag cgttcaaacc cgcgtgaatg
     2941 gccagctgcg gcagaatggg acgacggtgg acttcctctt cccgctcgac gtactcattc
     3001 acttcatttc gcaggtgatg actcttgaag ccggagatct gatcgccacg ggaactcccg
     3061 tcggcgtggg cccgctgcaa cccggagact ccgtcgaagt cgccgtggaa ggcgtcggca
     3121 ccctgaagaa tcccgtgctg aacctcgaat aggcggccgc ttacaaaacc attctgccc
//
LOCUS       DATMGX010000355         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_4602,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000355 DATMGX010000000
VERSION     DATMGX010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4602"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1418)
                     /locus_tag="VNR65_05670"
     CDS             complement(75..1418)
                     /locus_tag="VNR65_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ18201.1"
                     /translation="MLPPIIISLMAPFRSFFTAPVWEHVLALVTGMVLAPGKRTVSAA
                     LRIMGLGGTRDFALYHYVLNRARWNSRAIARPLLTMILDRFLPVGPVVIGLDDTIERR
                     WGRKIAARGIYRDPVRSSHGHFVKTSGLRWLSAMAMVPVPWARRRWALPFLTILAPSE
                     RYNTANGHRHKKLTDWARQAILQVRRWLPKHMIVVVADSSFAALDLIAAVRRHVCLVT
                     RLRLDASLFEPPPKRHASQKGRPPKKGRRLPKLSQRLEDKKTHWTRLSMPYWYGDERC
                     ILEIATGTAVWYHSGLPPAAIRWVLVRDPTDVRDPQAFLCTDLDATPVAILGWFVSRW
                     SIETTFQESRLHLGVETQRQWSDLAIARTTPAIFGLFSLVTLWAADPKIAAGLRPRSA
                     AWYDKRQPSFSDAIAAVRRIFWSAPSFSMSRNPLDTVKIPAALMVRLTEALCYAA"
     gene            1596..1880
                     /locus_tag="VNR65_05675"
     CDS             1596..1880
                     /locus_tag="VNR65_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18202.1"
                     /translation="MTDDDIIAFIESFKFGAGPVAVERRNRGFTLIHAATGYPVARLR
                     PIGRDDLVDILYWSLWKERWVPFGPFGRTAAPVKQAVRIIAEAAIFWTGA"
BASE COUNT          443 a          598 c          654 g          404 t
ORIGIN      
        1 tccttgagcc tacgtcctat cgaaaaagcc gagcaacttt ttcgggacat gctctaagct
       61 cgactttgcg gattttatgc cgcgtagcaa agcgcctcgg tgagtcgcac catgagggcg
      121 gcgggaattt tcaccgtgtc cagcggattc cgagacattg aaaaactcgg cgcgctccag
      181 aatatccttc gaacagcggc gatggcgtcg ctaaatgaag gttggcgctt atcataccat
      241 gcggcggatc tgggacggag gccagcggcg atcttgggat ctgccgccca aagcgttacg
      301 agcgaaaaga gcccaaatat cgctggggtg gttcgcgcga tggcgaggtc ggaccattga
      361 cgttgcgttt cgacgccgag gtgcaggcgg ctctcctgga acgtcgtttc gatggaccaa
      421 cggctcacga accatccgag aatagcgacg ggcgtcgcat cgagatctgt gcacagaaat
      481 gcctgcggat cgcggacgtc ggtgggatcg cgcacgagca cccagcggat cgccgccggc
      541 ggcaatccgg agtgatacca gaccgccgtt ccggtcgcga tctcgaggat gcatcgttca
      601 tcgccatacc aatatggcat cgacagtcgg gtccaatgcg tcttcttatc ttcgagtcgt
      661 tgggagagct ttggcaaccg acgacccttt ttgggaggtc gtcccttctg actggcatga
      721 cgtttcggcg gcggctcaaa cagactggcg tcgagccgca gtcttgtgac gagacagaca
      781 tggcggcgaa ccgcggcgat tagatcgagg gccgcgaaac tggaatcggc gacaacgacg
      841 atcatgtgct ttggcaacca tcgccgaact tgaagaatgg cttggcgagc ccagtcggtc
      901 agctttttgt gacggtgccc attcgcggtg ttgtaacgtt cggagggcgc cagaatggtc
      961 aggaaaggca aggcccaacg tcgccgcgcc catgggacgg gaaccatggc cattgccgag
     1021 agccaacgca aaccgctcgt cttgacgaaa tggccatgtg aggagcgcac ggggtcgcga
     1081 tagatcccgc gcgcagcaat ctttcggccc cagcggcgct cgatcgtgtc atccaacccg
     1141 atgacgacag gtccgacggg caagaaccgg tcgaggatca tcgtcagcag cgggcgggcg
     1201 atggcgcgac tgttccatcg cgcccggttg agcacatagt ggtagagcgc gaagtcacgg
     1261 gtcccaccga ggcccatgat acgcagggcg gcgctcaccg tgcgtttgcc gggcgcgagc
     1321 accatgccgg tgacgagcgc cagcacatgt tcccaaacgg gggctgtgaa gaagctccgg
     1381 aaaggcgcca tcagcgaaat gataattggc ggcagcatag gcaccgcgga ggatgaagga
     1441 aaagagcgat gttgggtcat cgcaacggta gaatcgaatc gcgctgaatt gtcgttgatg
     1501 cttttcaata tccgtctgcg gcattttgaa tcgggatcgc ggcaggcccg gacgaggaca
     1561 gttgtgggtc gatccgtcat aatggcgcaa tgacgatgac ggacgacgac atcattgcgt
     1621 tcatcgaaag cttcaaattc ggcgccggtc ccgtggctgt ggaacgacgc aatcgcggct
     1681 tcacactcat tcacgccgca accggctacc cggtcgctcg cctgcgtcct atcgggcgcg
     1741 atgatctcgt cgatattctg tattggtcat tatggaagga acgatgggtt ccgttcgggc
     1801 cgtttgggcg aaccgccgca cccgtcaaac aggccgtgcg catcatcgcg gaagcggcga
     1861 ttttctggac tggcgcctga acgcccaagc caaaaatccg caaagtcgag ctaagtccgc
     1921 gtttaaaaaa gacggctcca agcctcgcga tccgcgagcg cgtggcgtcc gactgttgcc
     1981 tttggtcgcc cccacagccg actgggagag tgatggcttc cagcggtccg ccgtctcatc
     2041 ccaccccttg accaggctca agcatgctcc atctgatgaa ccgcaaagct tgtttgatt
//
LOCUS       DATMGX010000356         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_423494, whole genome shotgun sequence.
ACCESSION   DATMGX010000356 DATMGX010000000
VERSION     DATMGX010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423494"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..525
                     /locus_tag="VNR65_05680"
     CDS             337..525
                     /locus_tag="VNR65_05680"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HWJ18203.1"
                     /translation="MRRKLEKFLQDESGAAAIELGLIAAGFCVALITLAGQMNEEVKI
                     VFERVRDYLRAFNLHAMG"
     gene            557..1033
                     /locus_tag="VNR65_05685"
     CDS             557..1033
                     /locus_tag="VNR65_05685"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HWJ18204.1"
                     /translation="MDSSLIATAAAALPVILVWAAAVDILTRTIPNTVVLLLTACFAI
                     FAVAAGMPLAQIGAHAACASAVLLFGFLLFSLSMVGGGDAKLLAAASLWLGFGNLLPF
                     LAATALAGGVLSLTYLAFSAAKIGLGQENVLARTVPYGAAIAAGALAVLPDWLASL"
     gene            1338..>2729
                     /locus_tag="VNR65_05690"
     CDS             1338..>2729
                     /locus_tag="VNR65_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18205.1"
                     /translation="MTNAALISRAKSIAASIEAMDFEDAAEQAHWIMVETNNDVSALL
                     DILAKEGAKKRPNEKLVTGCIFLLLQGLEQLRFRIEDKDQRSTDIVSRAIKHLEALVA
                     AKKLSVEFLLLILQQFVAAKLDMGEGLRTMMGQVIEEDASTMDEANLGALNTGFSEIA
                     AECDHDPFAIHGLLEQSADTMPHEMRKGLAMAAFGAPEPSLREASLGFLLNSSSHARQ
                     TLLELIRLAAPKGLVTPTMLRRMIAIRNWLPKSDQAQLDTAIKTCRKHGIACAPWPAA
                     KLGEIFVSGFDGSGAHTVIVTLQIGKKWGLAGLLVKLGFGVRDAWVQKGLSRSELKRT
                     LNHMRSEIVVFPSGADYLGSVVGHAVGMNAGMDALPPFGLLDFAEAVGLSEVNPQPSS
                     VDIMVAELCAGLEAERLTEKAIAETCADSENWVTEHSILQTWFEPNVASVVGAKRAST
                     KKRIAVLLTGPLRS"
BASE COUNT          570 a          743 c          803 g          613 t
ORIGIN      
        1 gaacttactt gtcgcctgca acgggggtta ggtcttgctg gagatcggaa accgtacgta
       61 ccttaccgca gtcgcaggag ggaatccaac ccagcgcaaa gctcgacttc gttcaggcga
      121 gctatcatcc cgcaagccga agatagcttt cgaccagttt cattcatgct tgactgcctg
      181 ggcgattcag gaagcgcaaa ggatcatccc taaagtgctg ctaaccatca aatgcaattc
      241 aacttggagc caaattcgaa ttaaagcgct tttaatagct cgagccctaa cgtgcaggga
      301 gacgccgctt ccgggcgtct acgttggagg agtgttatgc gtagaaaact tgaaaagttt
      361 ctacaagacg agtcaggggc cgcggctatc gagctcggcc tgatcgcagc cggcttctgt
      421 gtggccctca ttacgctggc aggtcagatg aacgaagagg tgaagatcgt ttttgaacgc
      481 gtgcgcgatt atctgcgcgc gtttaatctc catgcgatgg gctgaaagct tccgctaacg
      541 atctggttcg ccacttatgg attcatccct gatcgcgacg gcggcggcgg ccctacctgt
      601 gattctggta tgggccgccg cagtcgatat tctcacccgg acaatcccta acacggttgt
      661 tttgctactc accgcgtgtt tcgccatttt tgccgtagca gccgggatgc cgctggcgca
      721 gattggggcc cacgcggcgt gcgctagcgc ggttctgcta tttggatttc tgctattttc
      781 cctgtcgatg gttggaggcg gggacgccaa gcttctcgct gcggcgtcgc tctggttagg
      841 gttcgggaac cttcttccat ttctggctgc aaccgcgtta gcgggcggcg tcctttccct
      901 tacctatctc gcgttcagtg ctgcgaaaat tggtctcggc caagaaaacg tacttgcgcg
      961 caccgtaccc tacggtgccg cgatcgctgc aggcgccctg gccgtgctgc cggattggct
     1021 cgcctcactc tagcgcccca tcgccgaagg cggtcataag ccggtcgaac gggggctcgt
     1081 gaaaattcgt ataaccttcg tttgcgcgtg gcggcgagag cggaagagac gcgtgcgcgc
     1141 caatggcaaa agatcaacca tcgaaaatag cacgctcctc cgcatgcttt tactggggat
     1201 ggacctatgc tgagacaggc gcgccgcccc cgaatctgtc tcagatgacg acaatgcgct
     1261 atgtcgttgc gcaggcctgc gcgtaattcc agagcctttc tttcatcatt gcatcgttct
     1321 gaaggggagt tgaagccatg accaacgcag cgctcatcag ccgtgcaaaa tcgatcgctg
     1381 ccagcatcga ggcgatggac ttcgaagacg cggctgagca agcccattgg atcatggtcg
     1441 aaacgaacaa cgacgtgtcc gcgcttttag acatcttggc gaaggaaggg gctaagaagc
     1501 gccccaatga aaaactggtc acgggatgca tattcttatt gctacagggc cttgagcagc
     1561 tgcgtttcag aattgaggac aaagaccagc gaagcacaga catcgtgagc cgagcgatca
     1621 agcaccttga agccttggtc gcggctaaga aactttctgt agagttcttg ttgctgatct
     1681 tgcaacagtt cgtcgccgcg aagctcgaca tgggcgaagg gttgcgcacc atgatgggtc
     1741 aggtgattga agaggatgcc tcaacgatgg atgaggcaaa cttgggagca ttgaataccg
     1801 gtttttccga aatcgcagcc gaatgcgatc atgatccgtt tgcgatccat ggattgctcg
     1861 aacagtccgc cgacacaatg ccccacgaga tgcgcaaagg cttggctatg gccgccttcg
     1921 gcgccccgga gccgtcgctg cgtgaagctt cgttgggatt tttgttgaat agctcgagcc
     1981 atgccaggca aacgctgctt gagttgataa ggcttgctgc tccgaaaggg ctggttacgc
     2041 caaccatgtt gcgccgcatg attgccattc gcaattggct gcccaaatcc gatcaggcgc
     2101 agctcgatac cgccatcaag acctgccgca aacacggcat cgcgtgcgcg ccttggccgg
     2161 cggcaaaact tggcgagatt tttgtgagcg gtttcgatgg atcgggcgcg cacaccgtga
     2221 tcgttacgtt gcagatagga aagaaatggg gtctcgcagg gcttctcgtt aagctcggct
     2281 ttggcgtgag ggatgcctgg gtgcaaaaag gcttgagccg ctccgaactg aagcgaacgc
     2341 tgaaccatat gcgctctgag atcgtagtct ttccctcggg cgcagactat ctcggatctg
     2401 tcgttggcca tgccgtaggt atgaacgcag ggatggacgc tctgccgccc tttgggctgc
     2461 tcgactttgc cgaagcggtt ggcttgagcg aagtgaaccc gcagccttca tcagtcgaca
     2521 tcatggtggc ggagctttgc gctgggctcg aagctgaacg cctcaccgag aaggcgattg
     2581 ctgaaacctg cgccgacagc gagaattggg tgacggagca ttcgattctg cagacatggt
     2641 tcgagcccaa cgttgcctcc gtggtcggtg ccaagcgcgc ctcgacgaaa aagcgcatcg
     2701 cggtcttgct tacgggtcct ttgcgctcg
//
LOCUS       DATMGX010000357         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_313208, whole genome shotgun sequence.
ACCESSION   DATMGX010000357 DATMGX010000000
VERSION     DATMGX010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313208"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VNR65_05695"
     CDS             complement(<1..1154)
                     /locus_tag="VNR65_05695"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWJ18206.1"
                     /translation="MHEEKTISSGDAAGAVKELFHVCFWLARTYARKKKPADGLAFDV
                     SALSRRDEVLKKAFVQLKAQQAELDAKNGELTRLLTDKQNLDDELKKLREAVAAARKA
                     SEAQPDRHNYNEAETRDRYIDLLLREAGWALDKPDDLEFRVEGMPNNEGIGFVDYVLW
                     GADGKPLGLVEAKRTRKDARVGQQQAKLYADCLEARFGQRPVIFYSNGYEHWIWDDTR
                     YPPRQIGGFYKRDELELVIQRRSGRRKLGSEAISRKIVERPYQHRAIRNVARSFEQDG
                     ERKALLVMATGSGKTRTVIALVDLLMRAGWVKRVLFLADRVALVNQAAGAFKAHLPDA
                     APVNLITERTSEGRVFLSTYPTMMNLIDGKQEGKAKFGPGHFDLIVIDEA"
     gene            complement(1106..1435)
                     /locus_tag="VNR65_05700"
     CDS             complement(1106..1435)
                     /locus_tag="VNR65_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18207.1"
                     /translation="MSNFAFLAAEFSAVHEAATEAERQAAVSPTAAAFFAGKAVEVAV
                     KWAFRADPGLKLPYQDNISALLHEPSFRKAAGEAVFAKARYTSTRCAIALCTKKRPSA
                     LVTPLAP"
     gene            complement(1447..>2647)
                     /locus_tag="VNR65_05705"
     CDS             complement(1447..>2647)
                     /locus_tag="VNR65_05705"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HWJ18208.1"
                     /translation="GRNAVNAHWTVKRLADIFEIARGGSPRPIESFLTDDPKGLNWIK
                     IGYATASGKFIKVTKEKIRPEGLSKSRFVNSGDFLLTNSMSFGRPYIMATDGCIHDGW
                     LVLKPRDRKLVDQDYFYHLLGSNTIYEMLSARAAGSTVKNLNTEIVSNLEIKLPPLDE
                     QRRIAGILDKADELRRKRERAFNLLDDLTQSIFLEMFGDPIENTKQLPIAPLENVVSS
                     TRKITYGILKPGPDHAGGVPYVRVVDIRNSLIDVAGLRRTTTAIAAEYKRSTLRGGDL
                     LISIRGHVGRMAITPPECDGANITQDTARLAISGANTEFVKAQLETDPAKHWMAQRTK
                     GAAVKGINLGDLRQFPLILPPRELQDEFADKIGVVRMMLKKSTGQNDNLDFLFSSLQS
                     LAFSGRL"
BASE COUNT          541 a          774 c          740 g          592 t
ORIGIN      
        1 tgcgcctcgt cgatgacgat caggtcgaaa tgaccggggc caaacttcgc cttgccctcc
       61 tgcttaccgt caatcaggtt catcatcgtc ggataggtgg acaggaagac gcggccttcg
      121 ctggtacgtt cggtgatgag attgacgggc gcggcatcgg gcagatgcgc cttgaaggca
      181 ccggccgcct gattgaccag cgccacccga tcggcaagaa acagcacgcg tttcacccat
      241 cccgcgcgca tcaagagatc gaccaacgcg atcacggtgc gcgtcttgcc ggaaccagtc
      301 gccatcacca gcagcgcctt gcgctcgccg tcctgctcga aactgcgcgc gacgtttcga
      361 attgccctgt gctgataggg acgctcgacg attttgcggc tgatcgcctc ggaaccgagc
      421 ttcctccggc cgctgcgacg ctggatcaca agctccaact cgtcgcgctt gtagaagccg
      481 ccgatctggc gcggcggata gcgcgtatcg tcccagatcc agtgctcgta gccgttggaa
      541 tagaagataa ccgggcgttg gccgaagcgc gcctccaggc aatcggcata gagcttggcc
      601 tgctgctggc ctacccgcgc atctttgcga gtccgcttcg cctcgaccag acccagtggc
      661 ttgccatccg cgccccacag cacgtagtcg acgaagccga tgccctcgtt gttcggcatg
      721 ccctcgaccc ggaattcgag gtcgtcgggc ttgtccagcg cccagccggc ctcccgcagc
      781 aacaggtcga tatagcggtc gcgcgtctcg gcctcgttgt aattgtgccg atccggctgc
      841 gcttcgctcg ccttccgcgc ggcggcaacc gcctcacgca actttttcaa ctcgtcgtcg
      901 aggttttgct tatcggtaag gagcctggtc agttcgccat tcttggcgtc cagttcggcc
      961 tgctgcgcct tgagttgaac gaacgccttt ttcagcacct cgtcgcggcg cgacagggcc
     1021 gagacatcga aggcgaggcc gtcggccggc tttttctttc gcgcgtaggt gcgggcgagc
     1081 cagaagcaga cgtgaaacag ctctttcacg gcgccagcgg cgtcaccaga gctgatggtc
     1141 ttttcttcgt gcacagcgcg attgcgcagc gtgttgatgt atatctcgcc ttcgcgaaga
     1201 ccgcttctcc cgcggccttg cgaaagctcg gttcatgtaa gagcgcagag atgttatcct
     1261 gatagggcag cttcagcccg ggatcggcgc ggaatgccca tttcacagca acctcgaccg
     1321 ccttcccggc gaagaacgcc gcagcggtcg gtgagaccgc agcctgacgc tccgcctccg
     1381 tggccgcctc gtgaacggcg gaaaattcgg cggcgaggaa ggcgaagttg gacatatcgt
     1441 gtccgtttag agtctgcccg agaaggcgag ggattggagg gaagaaaaga ggaaatcaag
     1501 attgtcgttc tgcccagtgg atttcttcag catcatgcga acgacgccga tcttgtcagc
     1561 aaattcgtcc tgcaactctc taggtggcag tatcagcggg aattgtcgca aatcaccaag
     1621 gtttattccc ttaacggcgg cccccttggt gcgctgagcc atccaatgtt ttgcgggatc
     1681 ggtctcaagc tgagccttca caaattcggt gttggcacca gagatagcta aacgcgccgt
     1741 atcttgggtg atattcgcgc cgtcacattc tggaggagtg atagccatcc gccctacgtg
     1801 tcctcgaatt gaaatgagca agtccccccc tcgaagcgtc gaccgtttgt attcggccgc
     1861 tattgccgtg gtggtccgcc tcaatcccgc aacatcaatg aggctattcc gaatatcaac
     1921 cacacgcaca tacggaactc cgcccgcatg atctggccca ggcttaagaa tcccgtaagt
     1981 gattttgcgg gtggacgaaa cgacattttc gaggggcgct attggcaact gtttggtatt
     2041 ctcaatgggg tccccaaaca tttcaagaaa gatcgactgc gttaagtcgt cgagtaggtt
     2101 gaaggcacgc tcgcgcttgc gacgaagctc gtccgcctta tccaggatcc ccgcgattcg
     2161 tctctgctcg tcgagcggag gaagcttaat ttcgaggttc gatacaatct cggtgtttaa
     2221 attttttacc gtagagcccg cagcacgtgc agaaagcatc tcgtagatcg tatttgaacc
     2281 aagcaaatga tagaaataat cttgatccac taacttgcgg tcacgtggct ttagcacaag
     2341 ccaaccgtcg tggatgcatc cgtctgtcgc cataatgtaa gggcggccaa aactcatcga
     2401 gtttgttagg agaaaatcac cagagttgac gaatcgtgac ttagagaggc cttctggtct
     2461 aattttctcc tttgtgacct taatgaattt gccgcttgcg gtggcatagc caatcttaat
     2521 ccagttgagt cccttcgggt cgtcagtaag aaagctttcg ataggacgcg gtgatccgcc
     2581 acgggcaatc tcaaaaatat cggcgagacg cttgactgtc caatgggcat tcacagcatt
     2641 tcttcca
//
LOCUS       DATMGX010000358         3956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_105451, whole genome shotgun sequence.
ACCESSION   DATMGX010000358 DATMGX010000000
VERSION     DATMGX010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105451"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..573)
                     /locus_tag="VNR65_05710"
     CDS             complement(139..573)
                     /locus_tag="VNR65_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ18209.1"
                     /translation="MVRSFDHLTIVVRDPTPARAFFAVLGFREAVSCVIKGEVFARYM
                     GVPGIEAEHITLVLENASPRAEIQMLRYLHPEALPDEHIRQLNKIGMNHICFRVDDIE
                     AEVANIRAAGFQTRNEIMDFHSRKLVFLDGPEGVTVELSQWY"
     gene            859..1419
                     /locus_tag="VNR65_05715"
     CDS             859..1419
                     /locus_tag="VNR65_05715"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWJ18210.1"
                     /translation="MTSRVKPWTVRGSRYLIRERWLTARIDDCVTPAGVEVPYYVQEY
                     PGWVNVVALDKEDHLILVRLYRHALDDISLELPGGCIEEGESPIEAGARELLEETGYG
                     NPKRLTLIASLSPNTSTHANRVHTVLAEGVSLIGVPDDDGVEVLDVERVPYRQALDLA
                     MSGAIMQAAHIASIVLALNAGKKIEL"
     gene            1532..1915
                     /locus_tag="VNR65_05720"
     CDS             1532..1915
                     /locus_tag="VNR65_05720"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HWJ18211.1"
                     /translation="MPSGTHEHFEVAAQATWDGLPEQFRAALGNVTVHVTDFADAETL
                     KALHIRDPYDLLGLYHGVGRPFKGESDPVSLPDRILLYRIPILSYARRMGEPVDRVIR
                     HVLIHEIGHHFGFSDAGMALIESGK"
     gene            complement(2005..2808)
                     /locus_tag="VNR65_05725"
     CDS             complement(2005..2808)
                     /locus_tag="VNR65_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HWJ18212.1"
                     /translation="MRHCIIAFCLSFLIGPPAQAAQCGGDFDAFVNQISREATNAGVS
                     PAIAAQAFTRVSPDPAVLAFDRRQQGVFNKSFEYYAAIAVTRRRTELGRKLLRKNAVL
                     LSRIERDFGVPAPVIVAIWGLETHYGTRDLGDLPVIRTLATLAHDCRRTELFQSELLA
                     ALQILQRGDLSLRDMIGGNAGEIGQTQFLPTSYLKYGVDYDKDGRVNLRRSVPDVLAS
                     TANFLKANGWQKDVPFAEGTANFEVMRTWNQALLYRKAIVLLAERISEH"
     gene            3229..3717
                     /locus_tag="VNR65_05730"
     CDS             3229..3717
                     /locus_tag="VNR65_05730"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HWJ18213.1"
                     /translation="MTHRVLLVSLAMLAPAVALADTTPPTATHLVQEVYATGVQIYSC
                     EAGEQGPTWIFESPEADLANAPGQMVGTHFGGPSWKNNDGSVVIGEVVAKENAPKAGD
                     IPWLLLKAKSHEGSGAWSGVAYVRRTATSGGVAPKDGCEAARIGQQLRVPYKATYQFY
                     AE"
BASE COUNT          852 a         1171 c         1136 g          797 t
ORIGIN      
        1 tctcccctcg ccaattgcct gcgcccgccg atagaatagc gcaacctttt tggttccggc
       61 tctgccggct tagggttcta gaccacgtct ggatcaagaa gacggcgctc aacagacgca
      121 tttcgggtga ccccggcctc agtaccactg cgacagctcc accgtcacgc cctccggtcc
      181 gtcgaggaaa accagcttgc gcgaatggaa gtccataatc tcattgcgcg tctgaaaacc
      241 cgccgctcgg atgttcgcaa cctccgcctc gatatcgtcc accctgaagc aaatgtgatt
      301 catgccgatc ttgttcagct ggcggatatg ctcgtcaggc agcgcctctg gatgcaggta
      361 gcgcagcatc tgtatttcgg cgcggggcga cgcattctcc agcaccaggg taatgtgttc
      421 cgcctcgata ccgggaacgc ccatgtagcg ggcgaacacc tcgcccttga tgacgcatga
      481 gacggcctct cggaagccga gtaccgcgaa aaatgcgcgg gctggcgtgg gatcacgaac
      541 gacgatcgtt aggtggtcga aacttcggac cattgctagg aactccctat ggtactgtag
      601 ccccacattg aacatcgcag ctattgcagg aatttccaaa ccgcacgatg ttcaagggcg
      661 gcgtggaaag cctcgcccgt catcctttca ccatgagcca cggttccctg ccacttttct
      721 gctgctcacg acaccggcgc tcgccggctc gcgcgacaga ctggtggtga ccggaaattg
      781 cacgggacag caaaggcaag ctggcgaagt catcatcgaa ccagctaaat tgtctgagca
      841 ccaccgggag gacgacacat gacgagccgc gttaaaccat ggacggtccg aggatcgcgc
      901 tatctcatcc gggaacgttg gttgacggca agaatcgatg attgcgttac cccagcaggc
      961 gtcgaggtgc cctattacgt tcaagagtat ccaggctggg tgaatgtcgt cgctctcgac
     1021 aaagaggacc atcttatcct cgtccgcctt taccgccacg cgctcgacga catttccttg
     1081 gagctgccgg gcggctgcat cgaagaaggc gaaagcccga ttgaagcggg cgcgcgcgag
     1141 cttcttgaag aaacgggcta tggcaatccc aagcgtttga cgctcatcgc cagcctctcg
     1201 cccaatacgt cgacccatgc caatcgcgtt cacaccgtcc ttgccgaagg cgtgtcgttg
     1261 atcggcgtcc cagacgatga cggcgttgag gtgctggacg ttgaacgcgt cccatatcgg
     1321 caagctctcg atctggctat gagcggcgcg atcatgcaag cagctcatat tgcgtcgatt
     1381 gtgctggcac taaatgcggg caagaaaatc gagctgtaag ccgaacttgc cgccatccgt
     1441 gccgatcacg cgcgccagga ccattgggat ttgcaggaag cccacccaga ttctggacgt
     1501 gccgcattga gcttgcggga aagggggctg catgccttca ggtacacacg aacatttcga
     1561 ggtggccgct caggcaacct gggacggcct acctgagcag ttccgcgcgg cgctgggcaa
     1621 cgtgacggtt catgtaaccg atttcgccga cgcggagacg ctcaaggccc ttcacatccg
     1681 cgatccgtat gatctgctcg ggctctatca cggcgtggga cggccgttca aaggcgaatc
     1741 ggatcccgta agtttgcccg acaggatcct tctctaccgt atccccatcc tcagttacgc
     1801 ccgccggatg ggtgagccgg tggaccgagt tatccgccac gtgctgatcc acgagatcgg
     1861 tcaccatttc ggcttctccg acgccgggat ggccctgatc gaatccggca agtaacccat
     1921 actcaatcgg gccgctgagg ctaaaagctc ttagcgtgcc attaagttga ggccggagag
     1981 tggggaggaa gcgtggtatt gtcctcaatg ctcgctgata cgctcggcga gaaggacaat
     2041 agccttccga tacaacaagg cttggttcca agtgcgcatg acctcgaaat tggccgtccc
     2101 ttcagcaaaa gggacgtcct tctgccagcc gtttgccttg aggaaattgg ccgtcgaggc
     2161 gaggacgtcc ggcacgctgc gccgcaaatt cacacggccg tctttgtcat aatcgacgcc
     2221 gtatttgaga taggaagtgg gtagaaactg agtttggcca atctcgcctg cgttgccgcc
     2281 gatcatgtcg cgtaaagaaa gatcgccgcg ctgaagaatc tgcaaggcgg caagaagctc
     2341 gctctgaaac agttctgtgc gtcggcaatc atgagccagc gtggcaagag tccggatgac
     2401 tggcaaatcg ccaaggtccc ttgtgccata atgagtttca aggccccaaa tcgcgacgat
     2461 cacaggagct ggcactccga agtctcgctc gattcgtgac agcagcacgg cattcttgcg
     2521 taggagcttt cggccaagct ccgtgcgccg acgggttacc gctatcgcag cgtaatattc
     2581 aaagcttttg ttaaatacgc cttgttggcg gcggtcgaat gccagcacgg ctggatcggg
     2641 agaaacgcgg gtgaacgcct gggcggcgat ggctggcgac acgcctgcgt tcgtagcctc
     2701 acgggaaatc tgattgacga aggcgtcaaa atccccgccg cactgcgctg cttgcgcggg
     2761 gggaccaatg aggaacgaaa gacagaatgc tatgatgcaa tgcctcaagc aatatctctt
     2821 ttccagcctt gctaagattg gccgcttgga atgccacaag caacttcgtt acaatcttca
     2881 gcgtcgtgcc acaagccgtc gaatccgcga tcgtgaaaaa ccccgcaggc tacctccgag
     2941 aggccgaaag ccgaaggcgc ctttatcttg ctcatgaatt atgcgcgcag gcgccgcgtc
     3001 acactttggg aacagccgag gccatcgagg acaagcccga attgatcagc tcgctcctcc
     3061 cgggggacta ttttgccgag ccgctgcaag cgatctcttc acgctgaccg cccaagctgc
     3121 aatcgaagct ttcaacagtc ggatcgaacc cctgcaagcc gagttggaca cattacgttc
     3181 cggatcggtt tgtaaccggg tcaccgcttt ctcgaaggat tcttcttcat gacccatcgc
     3241 gttttgctcg tatccttggc tatgctcgcg ccagccgttg cgctggcgga caccacaccg
     3301 cccaccgcca cccatctggt tcaggaggtc tatgccaccg gggttcaaat ctacagctgc
     3361 gaagcaggtg agcaggggcc gacctggatc ttcgaaagcc ctgaggctga tctagccaac
     3421 gcgccaggtc aaatggtcgg cacgcatttt ggcggcccga gttggaagaa taatgacggc
     3481 agtgtggtca tcggcgaggt agtcgctaag gagaatgccc ccaaggccgg tgacattccc
     3541 tggctgctgc tcaaggcaaa gagccatgag ggaagcggcg cctggtcggg agtggcttac
     3601 gtgcggcgaa ccgcgaccag cggcggcgtc gcccccaagg atggctgcga agctgcgcgc
     3661 atcggccaac agctacgggt accttacaaa gcgacctatc agttctacgc cgaataaaaa
     3721 ggcaagcccg tgccagatgc aggctcactg caactaggta ggacgagttc gtgatccgga
     3781 tgttaaagtt cgccagcgcc atcgaggcct gagaaactcg ttcggcaagt cacccttcag
     3841 gccgagtgcg aacctgaagc ttgctggcct ggcacttcaa ttgttgaggg tgaagcttca
     3901 gtggcggcct aagccttgtg tcctcgccaa tcgcgctggc actttcccaa cggttt
//
LOCUS       DATMGX010000359         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_196887, whole genome shotgun sequence.
ACCESSION   DATMGX010000359 DATMGX010000000
VERSION     DATMGX010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196887"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1498)
                     /locus_tag="VNR65_05735"
                     /pseudo
     CDS             complement(206..1498)
                     /locus_tag="VNR65_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472234.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1691..>2095)
                     /locus_tag="VNR65_05740"
     CDS             complement(1691..>2095)
                     /locus_tag="VNR65_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18214.1"
                     /translation="KGTNVPPVRYEAASRAKLLDGGAMPQSCKVCSGGEKVPLGGERL
                     LTFRIAPIKKATFLQIEYTNLSPGPLPVQLRVDSQMPTNLSLPPTGPAGEVGSVTIEV
                     ETNQPGSLSTLTFSSPVATGPALVAVSVLGAH"
BASE COUNT          420 a          639 c          585 g          451 t
ORIGIN      
        1 tgacagttcc gcggcatgca atcttgagaa tttctggagt gattggtccg ccgttaagaa
       61 gcgggtttca tggatgaagc gcggaacagt tctgagcatt gaagagcggg ttgaggactt
      121 cttgcgactc taaggaagag cagaaggcga gagcggcgca ggccgcgagc agtggctgta
      181 gaaatgagcg caaaaaaagc ttcctgcgtt tcaagaagcg gagcagttgc cggagattaa
      241 agccgacggc ggccagtaaa gcgttggagc gatcacccgc gcggcctccc agccagttgc
      301 gtccgagcag tccgtcgttt ttagcgtgac caatgaccgg ttccaccact gcgcggcgtt
      361 tcagccatcg tttaattttc tccgtcacgc cacgtctctg tctggtgatg taaaccgcct
      421 tgtgtggcaa ggtgatgcga tgacccttgt atccttgatc gacggccaca cgttgcggta
      481 cctgccccac gattttgcga atgtcttcca gtgccgccgc cagcgtgtgg ccgtcgtacg
      541 gatttccgtg cagcgcctgc gcatgcagaa tgaagtgccc acccttggcc ggatgcagat
      601 tggtggccag ggaaactttg cagccgaact cgtacggcgc ccgcgccttg cccttgctga
      661 tgcattccac ttccgtcgca tgccaggaat agagataacc cggatcgccg cgccgctgct
      721 gcgcaatgcg ccaagccttg gccagcgcga tctccaaccg cttcacttgc ctctccgcca
      781 agccagaagc taccgccatc ttgcgccgta tgtcacgctg caaacgccgc aaacgtacac
      841 gcatgaattt gatctgcttc ttcgcgcgct tcttttgctg cgcgtgcagg taacgccccg
      901 ttttcatcgc cgcacgccgc gccacccgca cgtaggattg ccgcaaacgc aatccgcttt
      961 tcttggcttg cgcgcccaac tgttcgatcg ccgtcagcag caaaccgtgc tcggtcggat
     1021 gggctatcgc tttctcctgc accgtggtgt ccaccgccac cgcttcggtc gctttcacgc
     1081 tcagagctcc accgcggtac gctgctgcca gcgtttcctg cagcaacgtt tccagcgcct
     1141 gctcgcccac ccgatggcga aaatgcgtca tgccggaacg ctccagcgga aaggaatgct
     1201 gaaaatattc ctcgccgcaa aaatattgca tgtacggatc gcgctcccag cgctcgcaca
     1261 ccgcttcatc cgacaacccc tcgatatgct tcagcagatg caaccccacc actagccgga
     1321 tcggcaaccc aggtcgcccg gcctcgctgt agtacggcgc aaaatgcgct tccagccgcg
     1381 cccaatcgat caactcaccg agccgcacca gttcatggcg catgttgatg atgtttttca
     1441 gcttcgcacg aaacagctct tccaccccac tgtcctgcgg tttcactctc ggtttcatcc
     1501 gttcccccaa aaatagaatt gcaagatttc agcgctcctc acgtcaaaac cttgtaattt
     1561 cttccctgga aaaccaactg aattcactat cagctattca ctgtttgggg cttttaacgg
     1621 tagactcgca ggccgccaat cggcacaaca aggtgagtgc agatttgtgg gtggcgactc
     1681 acaaaaagag ctagtgggcg ccgaggacgg aaacggctac cagcgccggg ccggtcgcga
     1741 cgggagagga aaacgtcagc gtgctgaggg agcccggctg attggtttcc acctcgatgg
     1801 ttacggaacc aacctcgccg gccgggcccg tgggcggaag cgagaggttg gtgggcatct
     1861 ggctatcgac gcggagttgc acgggaagcg ggccggggct gagattcgtg tattcaattt
     1921 gcaggaaagt tgcttttttg attggcgcga ttcggaaggt gagcagtctt tcgccgccga
     1981 gaggaacctt ctcccctcct gagcagactt tgcaggattg cggcatggct ccgccatcca
     2041 acagcttggc gcgtgacgcc gcttcatagc gaacgggagg gacgtttgtg ccttt
//
LOCUS       DATMGX010000360         2931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_263454, whole genome shotgun sequence.
ACCESSION   DATMGX010000360 DATMGX010000000
VERSION     DATMGX010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263454"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..554
                     /locus_tag="VNR65_05745"
     CDS             396..554
                     /locus_tag="VNR65_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18215.1"
                     /translation="MTTIKEWLRAAGRDLDQRFTTARDKLTHAHRVASEKVTPVAHRA
                     AENVGSAY"
     gene            871..1182
                     /locus_tag="VNR65_05750"
     CDS             871..1182
                     /locus_tag="VNR65_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18216.1"
                     /translation="MTTADPAQRCLRIAADGVVNQRLDGLWQTWLGCLRAPLPGARMP
                     DARTDLVAGLLQSATPRMIVLRAMPVAEATALVPPRPSDKASLAANKRRLRSSRNGSS
                     L"
     gene            complement(1283..1702)
                     /locus_tag="VNR65_05755"
     CDS             complement(1283..1702)
                     /locus_tag="VNR65_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18217.1"
                     /translation="MEDLDEPIYFRNADWAVTGYGIERTDGSYPIEAKRLGELANYTD
                     QKLPDWPLHIARKVWNFDLFMEAFMIALWVHRGKYDPAFLANKDWVRDCKAVIEKKRR
                     NREGQVSISPPLQLLALTHLGGHYVGSLQNHPRDALV"
     gene            complement(1718..1885)
                     /locus_tag="VNR65_05760"
     CDS             complement(1718..1885)
                     /locus_tag="VNR65_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18218.1"
                     /translation="MPTPPEVADTLCYGLEGSTPIVPSAVSTASAIQKLISGVLIFLF
                     GLALRNMLKVK"
     gene            complement(1914..2276)
                     /locus_tag="VNR65_05765"
     CDS             complement(1914..2276)
                     /locus_tag="VNR65_05765"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ18219.1"
                     /translation="MMIRAVTLAAALAAVITLPAIAQDVKSPATTAQTAITDQEAKAW
                     VDKPVYSSDGNKLGEVLAIRRAADNKITELRADIGGFLGIGQHEIAVPAAHFYLHNDR
                     VMLDMTAAQAKELPKAAK"
     gene            complement(2411..2797)
                     /locus_tag="VNR65_05770"
     CDS             complement(2411..2797)
                     /locus_tag="VNR65_05770"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HWJ18220.1"
                     /translation="MSYYFSKTLSVPFDEGVSRVTAALKEEGFGIITEIDVKETFKKK
                     IGAEFRNYRILGACNPASAYEALMLEDKVGTMLPCNVIVQELSDGKVEVAAIDPVASM
                     QAIDNPALKQKAQAIQAKLRNVIEGL"
BASE COUNT          612 a          864 c          832 g          623 t
ORIGIN      
        1 cgagtcgaag ctcccccagc gctgcttcga gacggttacg gtcacgtccg cagatgacca
       61 cacggtctcc gacgagcagg aactctcttg ccattgccag gccaagcccc gcagatcccc
      121 cggtaaccac cacgcccaaa ggacgtgtcg cgccatcctc tccaggtctc atgcagtttc
      181 tcatcttgag aatttcaaca aatcgttagc ggcgttctca ggccacggcg gaagcgtctt
      241 ggcgggtgac aaaggcccac ggcatcagcc tcatcgatgc ggcgcatggg ccatccattg
      301 acgaggcgtg agagaacgct ggcgagatat gactcgatat atcactttgg ccagacttcc
      361 attaccctgg ccgtaccccc ggaaggagaa ctaaaatgac aacgatcaaa gaatggctga
      421 gggcggccgg acgcgacctc gaccaacgtt tcaccaccgc tcgcgacaag ctgacccatg
      481 ctcaccgcgt ggcgtcggag aaggttacgc cggtagcgca ccgcgcggcc gagaacgtcg
      541 gcagcgcata ttaagccgca aagcctcatg tcgaggcggc cgttgtttcc gccgcgccac
      601 acgtgaagca cggcgtcaag taagcggcca tccaggcgct tcgcttcgtc atcaaggctg
      661 agcggcagtt cctcgactat gttgaggcgc atcttccgaa ggaggacgca gcggacgccc
      721 ggcttccgct ggctgagccg gtcgagccct cgccggtggc aaagccgaag aaggcggcat
      781 gacaatgaag cgggcactaa cccaaagcgc cgtcgaaagc gccaagccgg aaatgaagcg
      841 cgtcgagatc ccggatgcca agcagatccg ttgactacgg ctgacccagc gcagcgttgc
      901 ctccggatcg ccgcggatgg cgtcgtcaac cagcgcctcg atggcctctg gcagacttgg
      961 ttgggctgcc tccgcgcgcc gctgcccggc gcccggatgc cggatgcgcg aaccgatctc
     1021 gttgccggct tgcttcagtc ggcgacccca cgaatgatcg tgctgcgcgc gatgcctgtc
     1081 gccgaggcaa ccgcgcttgt tccgcctcgc cccagcgaca aggcttcatt ggcggcgaac
     1141 aagcggcggc tccgctcgtc gagaaacggc tccagtctct gaaatcggca gcgcaagcca
     1201 tcggtgtcca tcgcgaaaca cgatagcttt attcgcggat tcttttcctg cgttcttcga
     1261 cccgtttatt tcgcgtcagg ccctaaacta aggcgtcgcg ggggtgattc tgcagagagc
     1321 ccacataatg gccccctaag tgagtgagcg cgagaagttg caagggcgga gaaatcgaaa
     1381 cctgcccttc tcggttgcgg cgcttctttt caatcacggc tttgcaatcg cgaacccagt
     1441 ccttattcgc gaggaaggcc gggtcatact taccgcggtg cacccaaagg gcaatcataa
     1501 aggcttccat gaacaggtcg aagttccata ccttacgagc aatgtgcagc ggccagtcgg
     1561 gcagcttttg gtcggtgtaa ttagccagtt cgccaagacg tttagcttcg atggggtagc
     1621 tgccgtcggt gcgttcgatg ccgtagcccg tcacggccca atcggcgttg cggaagtaga
     1681 ttggttcgtc aaggtcttcc atcttatgcc gaataaatca cttcaccttc agcatgttcc
     1741 tcagagcaag gccgaagagg aagattagca cgcccgagat gagcttctgg attgcgctgg
     1801 cagtggagac ggcgcttggc acaatgggag tgctgccttc gaggccgtag caaagtgtgt
     1861 ctgcaacttc aggaggtgta ggcacggtgc ccgcacctca atacgttgga tgcctacttc
     1921 gcagccttcg gcagttcctt tgcctgcgcc gccgtcatgt caagcataac gcggtcatta
     1981 tgcaggtaaa aatgggccgc cggcacagcg atttcatgct ggccgatgcc caagaagccg
     2041 ccgatatcgg cgcgcaattc ggtgatcttg ttgtctgctg ctcgccggat tgccaagacc
     2101 tcaccgagtt tattgccatc gctgctatag acgggcttgt cgacccatgc cttggcctcc
     2161 tgatcggtaa tcgccgtttg ggcggtagtg gcgggagatt tgacgtcctg agctattgca
     2221 ggcagcgtaa ttactgcggc aagagcagcc gcaagggtta ctgcgcgtat catcatttga
     2281 acttctcagt taaacaaacg ttaacgcata aagactgtgt catgcaccat gggctgccat
     2341 tgatagatat caaaagacgt tcggaataaa gtcgtcaata ttcggaaagt aggcgccctg
     2401 cggggctggc ctacaagccc tcgatgacgt ttcgtagctt cgcctgaatg gcttgcgcct
     2461 tttgtttcag cgccgggtta tcgatcgcct gcatggaggc gacagggtcg attgccgcaa
     2521 cttcaacttt cccatcgctg agttcttgaa cgatcacgtt gcaaggcaac atcgttccga
     2581 ctttgtcttc gagcatgagc gcctcgtagg ccgaagccgg gttgcacgct cccagaatcc
     2641 ggtaattgcg gaactcggcg cctattttct tcttgaaggt ttccttgacg tcgatctctg
     2701 tgatgattcc gaaaccttct tctttcagtg cagccgtgac gcgcgagacg ccctcatcga
     2761 agggaactga caatgtcttg ctgaaatagt agctcatttc tgatctccca atcgtcgata
     2821 agcttccgaa ggcggatgcg agcaattttc ccgttgatga caatgtcggc cctcaacggg
     2881 gcttcataat cacatccaac ccatgcccat gggcccatga atgagctggt c
//
LOCUS       DATMGX010000361         3217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_386676, whole genome shotgun sequence.
ACCESSION   DATMGX010000361 DATMGX010000000
VERSION     DATMGX010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386676"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..3186
                     /gene="secA"
                     /locus_tag="VNR65_05775"
     CDS             49..3186
                     /gene="secA"
                     /locus_tag="VNR65_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWJ18221.1"
                     /translation="MRQKTADWKAQLSHIQDKTELAGKLNEILPEAFAVVKNACRRLT
                     ERKAEIIVREHPLLWEMIPFDVQLIGGYGLHSGRIAEMATGEGKTLVATLPIYLNALT
                     GRGVHLVTVNDYLAARDSEWMGAIYKFLGLTVGCILHDQPPRVRREQYNCDITFGTNA
                     EFGFDYLRDNGMAARKEEQVQRGHYFAIVDEVDSILIDEARTPLIISGPSVHTYDEQY
                     AQWKPLVESLVRAQERLCARFLSEAEAQIKKLNPTDGSNPQNSEALEHEIGLLLFRVK
                     IGQPKSEGLMKLLEDPENLKLMNRAELDLHKDQKKVDLYREKEELLFAIDEKGFEADL
                     TEKGRNFVSPKDPEAFVLPELTTALHEIDAGPEPDARKRMEAKTKVQQDFETKAQKIH
                     AISQLLKAYSLYQLDVEYVIQENKVIIVDQHTGRLMAGRRWSDGLHQAVEAKEGVAIE
                     RETQTLATITIQNYFRLYTKLAGMTGTAETEAAEFQKIYNLDVTVIPTNRPLIRKENQ
                     DMVYRTEVEKFRNAANEIKECNAKGQPVLVGTISVEKSERLAAILKKMGVKHEVLNAK
                     NHEREAGIVSQAGRKNAVTVSTNMAGRGTDILLGGNPEFMTKDECLKKKVAERLGEEE
                     ARFVADEHFNYFTHNEQFYRVRRDMWDQLYAANKAFTDLEHDEVVGLGGLHIVATERH
                     ESRRIDNQLRGRAGRQGDPGSSRFFLSLQDDLLRIFGGEKMQNLMLRLGMEEDVPIES
                     KMITNRIRKAQEAVEAQNFEARKHLLEYDDVNNKQRQTVYGLRRQLLEGVEQKQRVMD
                     MMQGIVEEYIDRHCANDKHPDTWDLAALRNDILTQFGSKIDLKQLASLHRQEMMESIF
                     DQLQSKYQEKEDLVGPDVMRQTERIVMLQVIDNQWKDHLLSMDELKQGIGLRAQGQKD
                     PLVEYKRESFDYFTAMMDRIEDETVRYLYFLQVNTGSGPVLPFPDEDEEEEAEPEAPR
                     PDPNEQQRLAAKSTMEDFTRNIQRKKEREMEQLQFAGGDDSRGATPVITGQKTGRNDP
                     CPCGSGKKYKKCHGA"
BASE COUNT          789 a         1014 c          887 g          527 t
ORIGIN      
        1 cagatcaatg agatcgaggc cggactcaaa aaactttccg acgacgagct gcgccagaag
       61 accgccgatt ggaaggcgca actctcgcac attcaggaca aaacggaact ggccgggaag
      121 ctcaacgaga ttctgcccga agcgttcgcc gtggtgaaaa acgcctgccg ccggctcacc
      181 gaacgcaagg cggaaatcat cgtgcgcgag catccattgc tctgggagat gattccgttc
      241 gatgtgcaac tcatcggcgg ctacgggctg cacagcgggc gcatcgccga aatggccacc
      301 ggcgaaggca aaacgctcgt cgccaccctg cccatttatc tcaacgcgct caccggacgc
      361 ggcgtccatc tcgtgacggt caacgactac ctcgccgcgc gcgactccga atggatgggc
      421 gccatctaca aatttctcgg cctgacggtc ggctgcattt tgcacgacca gccgccacgc
      481 gtccgtcgcg agcaatacaa ttgtgacatc accttcggca cgaacgccga gttcggtttt
      541 gattatctcc gcgacaacgg catggctgcg cgcaaagaag aacaggtcca gcgcggtcat
      601 tacttcgcca tcgtggacga agtggactcc atcctgattg atgaagcccg cacgccgctc
      661 atcatcagcg gcccgtccgt tcacacttac gacgagcaat acgcccagtg gaagccgctc
      721 gtggaatcgc tcgtccgggc acaggaacgt ttgtgcgccc ggtttttgtc cgaagccgag
      781 gcgcagatta aaaagctgaa tccgaccgat ggttcaaacc ctcaaaattc tgaagccctc
      841 gaacatgaaa tcggcctgct cctgttccgc gtcaaaatag gccagcccaa atccgaaggc
      901 ctgatgaaac ttctggaaga cccggaaaat ctcaagttga tgaaccgggc cgaactggat
      961 ttgcacaagg accagaagaa agttgacctt taccgggaga aggaggaatt gcttttcgcc
     1021 attgacgaga aaggtttcga ggccgatttg accgaaaaag gccgtaattt tgtcagcccg
     1081 aaagacccgg aggcttttgt actgcccgaa ctcacaacgg cgctgcatga aattgacgcc
     1141 gggccggaac ccgacgcgcg caagcgcatg gaagcaaaga cgaaggtgca gcaggacttc
     1201 gagaccaaag cgcaaaagat tcacgccatc tcgcaattgc tcaaggctta cagcctttac
     1261 cagctcgacg tggaatatgt cattcaggag aacaaggtca tcatcgtgga ccagcatacg
     1321 ggccgcctga tggctgggcg ccgctggagc gatggtctgc atcaggccgt cgaggcgaag
     1381 gaaggcgtgg ctatcgagcg ggagacgcag acgctcgcga cgatcaccat tcaaaattac
     1441 tttcgccttt acaccaagct cgccggcatg accggcacgg cggaaaccga agcggccgaa
     1501 ttccagaaaa tctacaacct cgacgtcacc gtcattccca ccaaccgtcc cttgatccgc
     1561 aaagagaatc aggacatggt ctaccgcacc gaggtggaga agtttcgcaa cgccgccaac
     1621 gaaatcaagg agtgcaacgc caagggccag cccgtcctgg tcggcaccat ctccgtcgag
     1681 aaaagcgaac gcctcgccgc catcctcaag aagatgggcg tcaaacacga ggtcctcaac
     1741 gccaagaatc acgaacgcga agccggcatc gtctcccagg ccggccgcaa gaacgccgtc
     1801 accgtttcca ccaacatggc cggccgcggt accgacatcc tgctcggcgg caaccccgag
     1861 ttcatgacca aggacgagtg cctcaagaag aaagtggccg agcgcctggg cgaggaagag
     1921 gctcggttcg tggccgacga gcactttaac tacttcaccc acaacgagca gttctatcgc
     1981 gtgcgccgcg atatgtggga ccagctctac gccgccaata aggcattcac cgacctggaa
     2041 catgacgaag tcgtgggcct cggcggtctc cacatcgtcg ccaccgagcg ccatgagtcc
     2101 cgccgcatcg acaaccagct tcgcggccgc gccggccgcc agggcgatcc cggatcctcc
     2161 cgtttcttcc tctccctcca ggacgatctg ctgcgcattt tcggcggcga gaaaatgcag
     2221 aacctcatgc tccgcctcgg catggaagag gatgtcccca tcgaatccaa gatgatcacc
     2281 aaccggattc gcaaggccca ggaggccgtg gaagcccaaa acttcgaagc ccgcaaacac
     2341 ctcctcgaat acgacgacgt caacaacaag cagcgccaga ccgtctatgg attgcgccgc
     2401 cagttgctcg aaggcgtcga gcagaagcag cgagtgatgg acatgatgca gggcatcgtc
     2461 gaagagtaca tcgaccgcca ctgcgctaac gacaagcacc ccgacacctg ggacctcgcc
     2521 gccctgcgca acgacatcct cacccagttc ggctccaaga tcgatctcaa gcaactcgcc
     2581 agtctccatc gccaggagat gatggaatct atcttcgatc agttgcagag caagtaccag
     2641 gagaaggaag accttgtcgg acccgacgtc atgcgccaga ccgagcgtat cgtcatgttg
     2701 caggtcatcg acaaccagtg gaaggaccat ctcctgtcga tggacgagtt gaagcagggg
     2761 atcggcctcc gcgcccaggg tcaaaaagac ccgctggtgg aatacaagag ggaatccttc
     2821 gactatttca ctgccatgat ggatcgcatc gaagacgaga cggtgcgcta tctctacttc
     2881 ctgcaggtca acaccggcag cggtcccgtg ctgcccttcc ccgacgagga cgaggaggaa
     2941 gaggccgagc ccgaagctcc ccgtcccgac cccaacgagc agcagcgcct cgccgccaag
     3001 tccaccatgg aggatttcac ccgcaacatt cagcgcaaga aggagcgcga gatggagcag
     3061 ttacagttcg ccggcggcga cgactcacgc ggggccaccc ccgtcatcac cggccagaag
     3121 accgggcgca acgatccgtg tccctgcggt agcggcaaaa agtacaagaa gtgccacggg
     3181 gcgtaggcta ccattccaac ggtgaccgct cccgact
//
LOCUS       DATMGX010000362         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_392155, whole genome shotgun sequence.
ACCESSION   DATMGX010000362 DATMGX010000000
VERSION     DATMGX010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392155"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VNR65_05780"
     CDS             complement(<1..203)
                     /locus_tag="VNR65_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18222.1"
                     /translation="MMESKAALSFCFVVRLYRKTASHLSGRTRNPGLSLELQMLHRPA
                     TMPKVQQRKHYGGVEAVLSRLYRR"
     gene            241..657
                     /locus_tag="VNR65_05785"
     CDS             241..657
                     /locus_tag="VNR65_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18223.1"
                     /translation="MVDYKEIPESVLLQSAMPVVIQKFRNKALAYKLLKEVDLKFETV
                     KGARVSADRFLFDSLGEVSVELAGMPGLVLKFSGTGEYKREERRVYLTGFKILNDVTG
                     FANKLIEGTGISVGRSLSVTEQDDELLLSLFPEESV"
     gene            complement(722..1615)
                     /locus_tag="VNR65_05790"
     CDS             complement(722..1615)
                     /locus_tag="VNR65_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWJ18224.1"
                     /translation="MNFEISHKTIYRYSAPVSQSHHLLHLTPRSHMRQTVVRHSLIID
                     PAPASKTEMTDDFGNPASIITIEQDHTKLEIHSRSQVDVQAPWPVETARSASWNDVAA
                     QLRANLGPETFEAVQYSCPSRYIKPTRDIHKFARPSFPDGRPFLEAVQDLTARIYRDF
                     KYQSGATDAATSVEQVLKIRRGVCQDFAHLEIACLRTLGLAARYVSGYLLTYPPEGQP
                     KLVGADASHAWVSVWSPEWGWVDFDPTNNIIPKEEHIAIAFGRDFQDVSPVSGVLLGG
                     GEHEVEVAVDVNPAGNGSPAH"
     gene            complement(1612..>2433)
                     /locus_tag="VNR65_05795"
     CDS             complement(1612..>2433)
                     /locus_tag="VNR65_05795"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWJ18225.1"
                     /translation="LGSDAGAIEQAVRRIMTAPCRTHSLPGLESNILRVGSIVRERLS
                     LEVWQTLLRFANIASPKAVDIGDTVTLLDRLNAGIVTVASMNGLTAENMTRNYGWRFQ
                     DMGRRLERAFNLAELLLELFGEEKREDEETARLFFVLNVADSTITFRQRYLFAPILSL
                     VLDLLMVDESNPRGIGFQLRAIDEHLHALPQASPHAPHNEEQRLILDLLTRVRLAKVT
                     SLDHADAEGKRPELQELLSNLISGLPKLSEAITRRYFNLTEDDPRRVYTRFGRGQ"
BASE COUNT          514 a          667 c          740 g          512 t
ORIGIN      
        1 cgccggtaga gcctggatag taccgcctca acaccgccat aatgctttct ttgttgcacc
       61 ttgggcatcg tcgctggtcg atgcagcatc tgcaattcca aagacaggcc aggatttcga
      121 gtgcgtccgg aaaggtgcga agcggttttc cgataaagac gcacgacaaa acaaaaactt
      181 agagccgctt ttgattccat caaaaacggg ctctaacttc cagcaggagt gggggcatcg
      241 atggtggact ataaagagat tccggaatcc gttttgcttc aatccgctat gccggtggtc
      301 atacagaaat ttcgcaacaa ggccctcgcg tacaagctcc tcaaagaggt cgatctaaag
      361 ttcgagaccg taaaaggggc cagggtctca gccgatcgct tcctttttga cagccttggc
      421 gaagtctcag ttgagcttgc aggtatgccg ggcctcgtcc tcaaattttc cgggaccgga
      481 gaatacaagc gggaagagcg ccgtgtgtat ctgaccggtt ttaaaatttt gaacgacgtc
      541 accggttttg cgaataagct gatcgagggg acaggaatta gcgtgggccg gtctctcagt
      601 gtgaccgagc aggatgacga acttcttctc tccctttttc ccgaggaaag tgtctaaagc
      661 cttcgatcaa gtttaggcgc cttcaatcga tccgatcaaa aggcgcgcct tgatctatcc
      721 cttagtgcgc cggagaaccg ttgccggccg ggttcacgtc caccgccacc tcaacctcgt
      781 gctcgccgcc gccgaggagc acgccactca ccgggctcac atcctgaaaa tcgcggccga
      841 aggcaatcgc aatatgctct tccttgggga tgatattgtt cgtgggatcg aagtcgaccc
      901 agccccattc gggcgaccag actgaaaccc aggcgtggga ggcgtccgcg ccgacgagct
      961 tcggctggcc ctcaggcggg taggtgagca ggtagccgct aacatagcgc gcggcaagcc
     1021 caagggtgcg gaggcacgcg atttcgagat gtgcgaaatc ctggcagacg cctcgcctga
     1081 tcttcagcac ctgctcgacc gatgttgccg catctgttgc gccgctctgg tacttgaaat
     1141 cgcgatagat ccttgcggtt aggtcttgca ccgcttcgag gaagggacgg ccatcgggaa
     1201 agcttggccg cgcaaatttg tggatgtcgc gcgttggctt tatgtatcgc gatgggcagg
     1261 aatactgaac ggcttcaaac gtttccgggc caaggttcgc gcgaagctgg gccgcgacgt
     1321 cgttccagct cgcagaccgg gccgtctcaa cgggccatgg cgcctggaca tccacctgcg
     1381 accgcgaatg aatttcgagc ttggtatgat cctgctcgat ggtgatgatc gaggccgggt
     1441 tgccgaaatc gtccgtcatc tccgttttcg aggcgggagc cggatcgatg atcaggctgt
     1501 gacgcacaac ggtttggcgc atgtgcgacc ggggcgtcaa atgcagcagg tgatgggact
     1561 gcgaaaccgg tgctgagtat cgatagatgg tcttgtgact gatctcgaaa ttcattggcc
     1621 ccgcccgaat cgggtgtaga cgcgccgagg atcgtcttcg gtgagattga agtaacggcg
     1681 ggtaattgct tcggaaagct ttggcaagcc cgaaataaga ttactcagga gctcctgcag
     1741 ttcgggccgc ttaccctcag catcggcatg atcgaggctt gtaaccttgg cgaggcgcac
     1801 gcgggtcagg aggtcgagaa taaggcgctg ttcctcgtta tggggcgcgt gcggcgaggc
     1861 ctgaggcaag gcatgaaggt gttcgtcgat cgcgcggagc tggaacccga tgccgcgcgg
     1921 gttcgattca tcgaccatga gaagatcgag aacgagcgaa aggatcggcg cgaacaaata
     1981 gcgctgccgg aacgtgatgg tgctgtccgc gacattcaag acgaagaata ggcgcgccgt
     2041 ttcctcgtct tcacgcttct cttcgccaaa gagctcgaga agaagctccg cgaggttgaa
     2101 ggcgcgctcg agacggcggc ccatatcctg gaatcgccat ccgtaattcc gcgtcatatt
     2161 ctctgcggtt agcccgttca tggacgcgac tgtgacgatg cctgcgttca gccggtcgag
     2221 aagcgtcacc gtatcgccga tgtcaacggc tttcggcgag gcaatgttcg cgaaccgcaa
     2281 taacgtctgc caaacttcaa gtgacaggcg ctcgcgcacg atgctgccaa cccgcaggat
     2341 gttgctttcg aggcccggaa ggctgtgggt ccggcaaggc gctgtcatga tgcgcctcac
     2401 ggcctgctcg atcgcgccgg catcggagcc gag
//
LOCUS       DATMGX010000363         4048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_457792, whole genome shotgun sequence.
ACCESSION   DATMGX010000363 DATMGX010000000
VERSION     DATMGX010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457792"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VNR65_05800"
     CDS             <1..923
                     /locus_tag="VNR65_05800"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWJ18226.1"
                     /translation="EAALTGFVEASHSFNALANSAGLFVYDQGTSAELTATARNTSRP
                     WSGWAGTSQNRFSALDAEKLGERAVEKAVFRGNPVDLEPGAYTVVLERAAVADLVLQL
                     LGHMESRGADEGRSFLSKPGGGTKLNEKLLHEHVTITSDPADATVPETLWGEGGVAKR
                     RTVWFERGIARQASCSRFWAQKTGRDPLPRPHNLSMAGGTVPVEDMIRDIKRGILVTR
                     FWYVNTVDPRTLLSTGMTRDGNFLIENGKIVAPALNLRFNESPVSAFSNIEAMGPAER
                     TISGYGGDQAISVPPLMIKGFTFSSKSSGI"
     gene            1273..2523
                     /locus_tag="VNR65_05805"
     CDS             1273..2523
                     /locus_tag="VNR65_05805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ18227.1"
                     /translation="MPAPIFNLPPWFSMLSLAHFRLQRRALRSLIYLFWIYSFVSRAV
                     GIFSQIYIYELFNSVQLNIIAAIVTFTGIMIGFCICGVIAARYSLNAKHGFLLSFLFT
                     GLGLILLPLAHDVPQACGAVAIRGIGSGLFWLTIHTYELMKTSDHERDIYSTFLSAGD
                     QIVTLASPAFATLLIWISHRLGVGDFTLLFVALPPLLLLGLPALARLNDYRPAPIGRH
                     DLVYFFADRRNQAAQIYLMGGAATHILQQAVIPLAAFIVLGSALNVGGFNTVFSIAGA
                     VALLAVGSRRRPENRLFILGMSSIVLVALDVMLGFSMTLVTLIAYTIGTSITQPIMRV
                     SQHVIDLKTMDGMSHRTSDFYPAMILRDVALWVWRTLAALILLAFATDARTGQEVISL
                     GLYFIAGATIITYAGARILLTWRR"
     gene            complement(2574..3191)
                     /locus_tag="VNR65_05810"
     CDS             complement(2574..3191)
                     /locus_tag="VNR65_05810"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWJ18228.1"
                     /translation="MKAHTRLFTAALFPAAVLSSFAYAQATADIPPAKVQEGAYTVDP
                     QHTRVLFAVSHMGFTTWYGEFTQVSGNLTLTPKSVADSTFEIHIPTKTISTSNAKLDG
                     ELKSPQWLDAVKFPEIVFKAKGIERAGESSAKVTGDLTLHGVTKPVTLDVTFNGAGTN
                     PLDKKYTVGFEATGAIKRSDFGVKTYIPLIGDDVRIIISAGFEHK"
     gene            3320..4000
                     /locus_tag="VNR65_05815"
     CDS             3320..4000
                     /locus_tag="VNR65_05815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWJ18229.1"
                     /translation="MVARGVLSLSGMMIEKTVYFVRHGQSEDNAAPVFQSPDSPLSEI
                     GRTQAERVAERFCKLSFEALIASPFRRSSETAEIIAKVTGREVEYSELFVERVKPTCI
                     NGKPYDDEAANAVWRHWEESLYTSGMRVSDGENFDDLVRRADEALTFLGNRAERSLLV
                     VTHGYFLRTILARVLLGEMISGEIFRRFQHRASMENTGLTVVRYQGGFEEEPSWRVWV
                     YNDHAHLV"
BASE COUNT          795 a         1164 c         1142 g          947 t
ORIGIN      
        1 gcgaagccgc tctcaccggc tttgtcgaag ccagccatag cttcaatgcc cttgccaaca
       61 gcgcgggcct cttcgtttac gatcaaggca cgagcgccga actcaccgca accgcccgca
      121 acaccagccg cccctggtca ggctgggcgg gaacctcaca gaaccgcttc agcgccctcg
      181 atgcagaaaa gcttggcgaa cgcgccgtcg aaaaagcggt tttcaggggc aatcctgtgg
      241 atctggagcc gggcgcctac accgtcgtgc tggagcgtgc cgccgtcgcc gatcttgttt
      301 tgcaactctt aggtcacatg gaatctcgcg gtgcagacga aggccgaagc ttcctatcta
      361 agcccggcgg cggcacaaaa ctgaatgaga agcttttgca cgagcacgtc acgattacgt
      421 cggacccggc tgacgcgacc gtgcctgaga ccctttgggg agaaggtggc gtggccaaga
      481 ggcggaccgt ctggtttgag agaggcatcg ccagacaggc gagttgctcg cgcttttggg
      541 cgcaaaagac gggccgagat cccctgccac gtccccacaa cctgtcaatg gcgggtggta
      601 cggtgcccgt tgaagacatg atacgggaca tcaagcgagg catcctggtg acgcgcttct
      661 ggtatgtgaa tacggtagac ccgagaactt tgctctctac cggcatgact cgcgacggca
      721 acttcctcat tgagaacgga aagatcgtag ctcccgcgct gaacctccgg ttcaacgaaa
      781 gcccggtttc cgccttcagt aacatcgaag ccatgggccc cgccgagcgc acgatcagcg
      841 gctatggcgg cgatcaagcg atctcggttc cgcccctcat gatcaagggc ttcacgtttt
      901 cctcgaagtc gagcggaatc tgatgccggc ggtcggagcg ttcgtctcct ttccgccagg
      961 aatttcccaa gcctggtcgc catcctgtga gggtgtacaa tgggttaaga ttggttttgt
     1021 tttgtaaaag gcccctaagt aagtatataa agagttttct gtcgcttttg aggacgctat
     1081 gggcgaaggc gtcgtcattt catggcggtt ccttatgcga aagtgatgtc cgcaagggcg
     1141 gcaaaccgcc gggatctttc gaggtgggtc gcttgccaca catggggtag ggcagcgtcg
     1201 ggcgtccctg gcgttttgcg cgccaccctt aaaaaagcaa agattgcgct cgcttatcgg
     1261 cttcgcgcaa agatgcctgc gcctattttc aacctgccac catggttttc gatgctttcc
     1321 ctggcacatt tccggctaca gcggcgcgcg ctgcgttcgc tgatttatct gttttggatc
     1381 tacagttttg ttagcagagc tgtcggtatt ttttcacaga tttatatcta cgaactattt
     1441 aacagcgtgc agcttaatat cattgcagca atagttactt ttaccggcat catgataggc
     1501 ttctgcattt gcggcgtgat tgccgcccgt tacagcttga atgcaaagca cgggtttctt
     1561 ctcagcttcc tctttaccgg gttaggtctc atccttctgc cgctcgccca tgatgtgccg
     1621 caggcttgcg gcgccgtcgc gataagaggg attggcagcg gcttgttctg gctcaccatt
     1681 cacacctatg agcttatgaa aacaagcgat catgagcgcg acatctattc gacgtttctg
     1741 tccgccggcg accagatcgt tacattggca agccctgctt ttgcaaccct tttgatctgg
     1801 atttcgcacc gccttggcgt cggcgatttc acgctcctct tcgtggcatt gccaccgctc
     1861 cttttgctcg ggctcccggc ccttgccagg ctgaacgact accgtccggc cccgattgga
     1921 aggcatgatc tcgtgtattt tttcgcagac cggcgaaatc aggcggccca gatttatctc
     1981 atgggcggtg cggcaacgca tatccttcag caggccgtta tcccgctcgc ggctttcatc
     2041 gtgcttggca gcgcgctgaa tgtcggcggt ttcaatacgg tcttctcaat tgccggagca
     2101 gtcgccttgt tagcggtggg ttcgcgccgc cgtccggaaa accgcctctt catactcggc
     2161 atgagttcta ttgttcttgt tgcgctcgat gtcatgctcg gcttttcgat gacgcttgtc
     2221 acactcatag cgtacacgat cggcaccagc atcacgcagc ccatcatgcg cgtttcccag
     2281 cacgtcatcg accttaaaac catggacggg atgagccatc gcacaagcga tttttacccc
     2341 gcaatgatcc tgcgcgacgt tgccttgtgg gtgtggcgga cgcttgcggc gcttattctt
     2401 ctcgcgttcg ccaccgacgc ccgaacgggg caggaggtta tttcccttgg cctgtatttc
     2461 atcgccggcg ccaccatcat aacctatgcc ggcgcgcgga tccttctcac ttggcggcga
     2521 tgaggctttc tcgcaaaggc agcgttcaag ccatcatccc cgcgccgcaa aacttattta
     2581 tgctcgaatc cggcgctgat gatgatcctg acgtcatcgc cgataagcgg aatataagtc
     2641 ttaacgccga agtcgctccg cttgatcgca cccgtggcct cgaagccgac cgtgtatttt
     2701 ttgtcgagcg ggttcgtgcc cgcgccgttg aacgtcacat caagggtcac aggctttgtc
     2761 acaccgtgca acgtcaggtc acctgtaacc ttggccgaac tctcgcccgc gcgttcgatc
     2821 cccttcgcct tgaaaacgat ctcgggaaat ttcacggcat cgagccactg agggctcttg
     2881 agttccccgt ccagcttcgc attgctggtc gaaatggttt ttgtcgggat gtggatctcg
     2941 aacgtgctgt cggcgaccga cttcggcgtg agagtcaaat tgccggagac ttgagtgaat
     3001 tccccgtacc aggtcgtaaa gcccatatgg gagaccgcga aaagcacgcg cgtatgttgc
     3061 gggtcgaccg tataagctcc ttcctgaacc tttgccggag ggatgtccgc cgtcgcttgt
     3121 gcgtaagcga aggacgacag aaccgcagca ggaaaaagag cagccgtgaa aagtctggtg
     3181 tgcgctttca tcgtgagatg cttcctttgt tgaactctgt tggacttacg tcacgcggcg
     3241 ggggcggaga tttcaaggcg ctggggcaag ggcggcattt tgcgcatccg tcccctcggg
     3301 gatattctcg acacggataa tggttgcaag aggcgttctc tcactgtcag gcatgatgat
     3361 agaaaaaact gtatacttcg tccggcacgg gcaaagcgag gacaatgctg cgcctgtgtt
     3421 ccagtccccc gactccccgc tgagcgagat agggcgcacg caggccgagc gggtcgctga
     3481 gcggttttgc aagctttctt ttgaagcgct cattgccagc ccctttcgac gctcgagcga
     3541 gaccgccgaa atcatcgcga aggtaacggg cagggaggtg gaatattcgg agctttttgt
     3601 cgaacgcgtc aagcccacat gcatcaacgg caaaccttat gatgacgaag ccgccaacgc
     3661 ggtctggagg cactgggagg aaagtctcta cacatcgggg atgcgggtct cagacggcga
     3721 aaacttcgac gatctcgtcc ggcgcgccga cgaggcactc acctttcttg gaaaccgcgc
     3781 cgagcgttcc ctgctcgtgg tgacgcacgg atattttttg cgaaccatcc tcgcccgcgt
     3841 tttgttgggg gagatgattt ccggcgaaat cttcaggcgc tttcagcaca gagcatcgat
     3901 ggagaacacc ggacttacgg tggtccgcta ccaagggggc ttcgaagaag agccaagctg
     3961 gcgtgtttgg gtttataacg atcacgctca tttggtctga acagcgcgtt cattatttgg
     4021 gaacccgtcc gtgccgccgt ccgaagcc
//
LOCUS       DATMGX010000364         6047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_269933, whole genome shotgun sequence.
ACCESSION   DATMGX010000364 DATMGX010000000
VERSION     DATMGX010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269933"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VNR65_05820"
     CDS             complement(<1..1048)
                     /locus_tag="VNR65_05820"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HWJ18230.1"
                     /translation="MDLQISLIDQLESLSASKDLSRRAEILRKVTDLFVLGSGNFSED
                     QVELFDNVMGELIKNIECAARAQFGSRLAKLSDAPRKVIRELAFDDAIEVAGPVLSHC
                     ARLDEYALVENARTKSQDHLLAISGRKTLVEAVTDILVERGNQTVVSCTARNSGARFS
                     DFGVSTLVQKARDDGDLALCVWSRPDIPRQNLVKLFVEASEAVKSQLVEADPRRAELI
                     RKVVAKASDQIQTKARVGSDDFAQAKSYVTSLYSSGKLDEGQLVTFANEGNFDKVTVA
                     LSLMCSLPLGLVERAFVQSQTEQILVLAKAIELSWVTTVALLLLQAGVNGSSRQQLDL
                     CFASFSRLQPKTAQT"
     gene            complement(1099..5622)
                     /locus_tag="VNR65_05825"
     CDS             complement(1099..5622)
                     /locus_tag="VNR65_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HWJ18231.1"
                     /translation="MAALGILFSALIVILFLVDLRTRYHNAIDAAKNEALKDAAILSD
                     NTVLTFEAVDRVLREAELIRLGGISGNYATPDAMNAALRHVVQTSPFVVAVGWTNASG
                     DLLAHSYEKTPPRANIADLPHFAAQREGGADRLFIAEPFSSSAGDRWLIAASRRLNNA
                     DGSFAGVVTAPLDPSYFNRTFRSFNLNNGGAILLLHRSGQLLAREPVVKRAIGKSFAA
                     GPLLTEYVPRSDAGSYETVSVVDGTARIAGYKAVPGLPLVLLVSYARAAVLAPWYQHL
                     YQFGSLLILIVVSILAGTFVLVRQTSSLAEKTAAVEATNTRFDAALSNMSQGLCLFDA
                     NKNLVIANSRFREMYGLPEEAVRPGTSLSDILEHHTERGEKSELSVEEHVQTMPAELN
                     QVFSLADGRVISIKRTPMSNGGWVATHDDITERKQSETALLKSKAMLHSTLAALSEGV
                     VVRDVNGRIISCNPAAERILGIRSGDLSDATSVVSGRQTIHEDGRDFPEVEHPAMMAL
                     ATGAPQLDVIMGLRSDAGSITWISINSVPIFADGKSEPTSVVTSFSDITARKQAQEVL
                     REAISASPDALVIYDENDRLITCNEAYKQIYAESAAAIYPGALFEDVLLCGLENNQYP
                     EAGETPAQRSAWLAERIRLHRAPSTDAIQRLSNGRWIQLRERRTAAGLTVGFRIDVTE
                     LHQKTAKLQAVIDNFPGGISFLDADYNIVACNQTFRTLLDLPDDLFQDGLPSLEMVLR
                     ANAMRGEFGPGDPDEQVRERMALAQNEGPQLFERTRPNGTVLEIRGTPIKGGGSIRTF
                     MDITARHTAEKQLFDSERRAQEKSATLELTLAYMSQGLSMFDASGQLIVWNDRYATMY
                     GLPPDLLKEGVSVGTIAEHMSRAGYLGTDEPDWRQKLADKNAFGSTLRFGDGRVIRIV
                     RTPIEGSGWVATHEDITEEMRAEASLFQQSAELARINMQFDAALSNMTQGLCMFDNQK
                     RLVVWNDRFAELYQVPPALLKVGTPYEAIVADRIGRGMLKGEASELTVNTKVAELAQL
                     PANARRIDELADGRFMLVARQPMEGGGWVATVEDITEQRRAEAEIVHLARHDVLTGLA
                     NRAEFNAKLEEASKRLKRNGGAVSVMMLDLDKFKAVNDALGHPAGDQLLVEVARRLQS
                     SVRETDVLARLGGDEFAIIQEGGPNQHEGAIALALRIINAITQPFDLNGQQASVGTSI
                     GIVHAPEHGAEPEELLKKADLALYNVKASGRNDFQVFRSEMLEVAHAQQSAEGELRGA
                     ISRDEFELHYQPVIDVKSRQLRGVEALIRWRHPTKGLITPDKFIPLAESTGLIVQLGE
                     WVLQQACTDAASWPAHIGVAINISAVQFRKGNLFDVILCTLVETGLSPERLELEITET
                     SLLENQEAHLVTIRQLKNLGISVALDDFGTGYSSINYLTIFPFDKIKIDKSFTQGALN
                     RRDCKAVVASTLALARGLGTVTTAEGIETEEQFEYMREAGVDLVQGYLFGRPVPISQL
                     DLHNTNLPKEMVA"
     gene            complement(5836..5901)
                     /locus_tag="VNR65_05830"
     tRNA            complement(5836..5901)
                     /locus_tag="VNR65_05830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5876..5878),aa:Arg,seq:acg)
BASE COUNT         1299 a         1710 c         1650 g         1388 t
ORIGIN      
        1 cagtttgggc agtctttggt tgaagccggg agaagctcgc gaagcataag tcaagctgct
       61 gccgtgagct cccgttaacg cctgcttgta acagcaagag agccactgtc gtcacccatg
      121 acagctcgat agctttggcc aggacaagaa tctgctccgt ttggctctgc acaaaagcac
      181 gttcaaccag cccgagcgga aggctacaca tcaacgacag ggctacggtg accttgtcga
      241 agtttccttc attggcgaac gtgaccagtt ggccttcgtc tagctttccc gaggagtaaa
      301 gggaggtcac ataggacttc gcttgcgcaa aatcgtcgga acctacccgc gctttagtct
      361 gaatctgatc cgatgcctta gcgactacct ttctaatcaa ctcagccctt cgaggatccg
      421 cttctaccag ctggcttttt acggcttccg atgcctcgac gaataacttt acgaggttct
      481 ggcgcggaat gtctggacgc gaccagacgc acaaagcaag atcgccatcg tcacgagcct
      541 tttggacgag ggtagaaaca ccgaaatccg agaaccgtgc accgctgttt ctcgcggtac
      601 aggaaacgac agtttgattg cctcgttcca ccaagatgtc cgtcactgct tcaaccaagg
      661 tcttgcggcc ggagatcgcc aatagatggt cctggctctt ggttctcgca ttctctacca
      721 gtgcatattc gtcaagccgt gcacagtgcg agaggactgg tccggcaacc tcgatcgcgt
      781 catcgaatgc gagttctcta atgaccttgc gcggcgcgtc agagagtttg gcgagccggc
      841 tgccaaactg cgcacgggcg gcacactcaa tgttcttgat aagctcaccc atgacattgt
      901 cgaacagctc gacttgatct tccgaaaaat tgcccgatcc gagcacaaag aggtcggtca
      961 ctttccgcag aatctcggca cggcgcgaaa gatctttgct ggctgatagg ctctcaagtt
     1021 ggtcaatcag actaatttgc aggtccatgt tcattgccca atacagtgat ggaaattgtt
     1081 agaatttggg tcttgcggct atgccaccat ttctttaggc aagttcgtat tgtgcaggtc
     1141 gagttgagag atcggaacgg gccggccaaa cagatagccc tgtaccaggt ctacccctgc
     1201 ctcacgcatg tactcgaact gctcttcggt ctcgatcccc tcagccgtcg ttacagtacc
     1261 tagaccccga gccagtgcga gcgtcgaggc caccaccgcc ttacaatctc gtcggttgag
     1321 ggcgccctgc gtgaaggact tgtcgatctt gatcttgtcg aacggaaaaa tggtcagata
     1381 gtttattgag gagtatccgg tgccaaaatc atcgagtgcc accgagattc ccaaattctt
     1441 caactgccgt atggtcacca gatgcgcttc ctggttctca aggagagagg tctcggtgat
     1501 ctccagctcg agccgttcgg gcgacaggcc cgtttccacc agggtgcata gtatgacgtc
     1561 aaagaggttt cccttgcgga attgaaccgc cgagatgttg atcgcgaccc cgatgtgagc
     1621 gggccacgaa gccgcatccg tgcaggcctg ctgcagaacc cattcgccga gttgcactat
     1681 caggccggtc gattccgcga gcgggataaa cttgtccggc gtaatgagac ccttcgtcgg
     1741 atgccgccag cggatcagtg cctcgacgcc gcgtagttgg cgtgacttta cgtcgatcac
     1801 cggctggtag tgaagctcaa attcatcccg cgagatggca cccctcagct cgccctctgc
     1861 ggactgctga gcatgcgcaa cctcaagcat ttcagatcga aatacttgga aatcatttcg
     1921 tccactggct ttcacattgt agagtgcaag atcagctttc ttcaaaagtt cctcgggttc
     1981 agcgccatgt tccggggcat ggacgatccc gatgctggtg cctacgctgg cctgctgccc
     2041 gttgagatcg aagggctgag tgatggcatt tatgatccta agcgcgaggg caatagcgcc
     2101 ctcgtgctga ttgggtccgc cctcctggat gatcgcaaac tcgtcgccgc ccaagcgtgc
     2161 cagcacgtcg gtttctcgaa ccgaagattg caggcgtcgg gccacttcaa ccagcaattg
     2221 gtcgccggcg ggatggccaa gcgcgtcatt gacggccttg aatttatcga ggtcaagcat
     2281 catcacagag actgcgccac cattccgttt aaggcgcttg ctcgcttctt ccagctttgc
     2341 attgaattct gcgcggttgg cgagtcccgt cagcacgtca tgccgcgcca ggtgaacgat
     2401 ctctgcctcc gcgcgtcgct gctcggtgat gtcctcgacg gtagcgaccc agccgcctcc
     2461 ttccatcggc tgccgagcga cgagcatgaa ccgtccatcc gccaattcat cgatcctgcg
     2521 ggcattggct ggaagttgag ccaattcggc gacctttgta ttgacggtaa gttcgctggc
     2581 ttctcctttt aacatgccgc ggccgatacg atcggcgaca atagcctcgt aaggcgttcc
     2641 aactttcaac aaagccggtg gcacctggta cagttccgca aaacggtcat tccagacaac
     2701 tagccgcttc tgattgtcaa acatgcagag cccttgtgtc atgttgctca aggcagcgtc
     2761 gaattgcatg tttattcgcg cgagctctgc ggattgttga aatagcgacg cctcagcgcg
     2821 catctcttcg gtgatgtcct cgtgggttgc cacccatccg ctaccctcga tcggcgtccg
     2881 gacaattcgt atcacacgtc cgtcgccgaa cctgagcgtc gatccgaaag cgtttttgtc
     2941 ggccagtttt tgccgccaat ccggctcatc tgttcctaga taaccggccc gggacatgtg
     3001 ctctgctatt gtgccgacgc tgacgccctc cttcaggagg tcgggcggga ggccatacat
     3061 cgtggcatac cggtcattcc agacgatcag ctgcccgctg gcatcgaaca tcgatagacc
     3121 ctggctcata tacgcaagcg taagctcaag tgtcgctgac ttttcttgtg ctcgccgctc
     3181 gctgtcaaag agctgctttt cagccgtgtg ccgcgccgtt atatccatga aggttctaat
     3241 ggatccgccg cccttgatcg gtgtgccgcg aatctccaac acggtgccgt tagggcgcgt
     3301 tcgctcgaac agttgcggtc cttcgttttg agcaagcgcc attcgctcgc ggacctgttc
     3361 gtccggatcg cccgggccaa actccccgcg catggcgttt gcgcggagga ccatttcaag
     3421 cgaaggcagc ccatcttgaa aaaggtcatc cggaaggtcg agcaatgtgc ggaacgtttg
     3481 attgcaggcc acgatattgt agtccgcatc gaggaacgag attccgcctg gaaaattatc
     3541 gatcaccgct tgcaatttgg cggtcttttg gtggagctcg gtcacatcaa tccggaagcc
     3601 cacggtgagt cctgctgcgg ttcgacgctc acggagttgg atccaccggc cattcgacaa
     3661 ccgctgaatc gcatccgtcg agggtgcccg atgcaatctt atcctttccg cgagccatgc
     3721 ggaccgttgc gcgggtgtct cgccggcttc gggatactga ttgttctcaa ggccgcaaag
     3781 cagaacgtct tcgaacaatg cgccaggata aattgccgcg gcggactcgg cgtatatctg
     3841 cttatatgcc tcgttgcagg ttattagccg gtcgttttca tcatagatta caagagcgtc
     3901 agggctggcg cttattgctt cccttaggac ctcctgggct tgcttacgcg ccgtaatgtc
     3961 agaaaacgag gttacgaccg acgtcggctc gcttttcccg tcggcaaaga ttgggaccga
     4021 atttatcgag atccacgtaa tggatccggc gtcactccgt agccccataa tcacatcaag
     4081 ctgtggcgcg ccagtcgcaa gcgccatcat cgccgggtgt tcaacttccg ggaagtcgcg
     4141 gccatcttca tggatagtct gtcggcccga aacgacagag gttgcgtctg ataggtcgcc
     4201 ggacctgatc ccgagaattc gttcggcggc cggattgcag gaaataatgc gcccattcac
     4261 atctcggacg accacgcctt cgctaagcgc cgccaaggtc gagtgcagca tcgccttgct
     4321 cttcagaagg gccgtttcgc tctgctttcg ctcagtgatg tcgtcatggg tggccaccca
     4381 gcctccgttc gacatcgggg tgcgtttaat agaaattaca cgcccatcgg caagtgagaa
     4441 aacctgattg agctccgcag gcattgtttg cacgtgctct tcgacggaca actcgctctt
     4501 ctcgccgcgc tcggtgtggt gttccaaaat atcagacagg gacgttccgg gtctgacggc
     4561 ctcttctggg agcccgtaca tctcacggaa tcggctattc gcaatcacca ggttcttgtt
     4621 cgcatcaaac aggcacagac cctgagacat gttgctcaag gcggcatcga accgcgtgtt
     4681 agttgcctca acagcggcag ttttctcagc gaggctgctt gtttgcctga cgagcacgaa
     4741 ggtgccggcc aatattgaga caacgatcag aatgagcagc gacccaaatt gatataggtg
     4801 ttggtaccat ggagccagca cggctgcgcg cgcgtaactg acgagcaaaa caagggggag
     4861 gcccggaacc gccttgtatc cggcgattcg cgccgttcca tcgacgacgc ttactgtttc
     4921 gtaagaccca gcgtcagatc ttggaacata ttcggttagt agtggaccgg ccgcaaatga
     4981 tttcccgatt gcccttttta ctaccggctc tcgcgcgagg agttgccccg atcgatgcag
     5041 taacaatatg gcgccgccat tattgagatt gaaggaacga aaggttcggt tgaagtagga
     5101 gggatcgagc ggagcggtta cgacgccggc aaagctgcca tcggcgttat ttagacggcg
     5161 cgacgccgcg ataagccatc tatcgccggc ggaggaactg aaaggttcag cgatgaacag
     5221 tcgatctgct ccgccttcac gctgtgcggc aaagtggggc aagtctgcaa tattcgctcg
     5281 aggcggcgtt ttctcgtagg aatgggcgag tagatcgccc gaggcattag tccacccgac
     5341 ggctacgacg aaaggagaag tttgcaccac atggcgcagc gcagcattca ttgcatcggg
     5401 cgttgcgtaa tttccagaga tgccaccaag acgaatgagc tcggcttcgc gtagaacgcg
     5461 atccacggct tcaaaagtga gcaccgtatt gtcggagaga attgccgcgt ctttcaatgc
     5521 ttcgttcttc gccgcatcga tagcattgtg gtatcgggtc cgcagatcga ccaaaaataa
     5581 aattacaatc agcgcactaa aaaggatgcc gagcgcagcc attgaatgct ttggagatcg
     5641 ggcgaaagct tgcagtgatt tccacatagc ccgatgatag acgggcggtc atttgagaac
     5701 ccttaattct atgacggcaa tgatttgata acgttaatat cctccgcatc cttcaaaaag
     5761 gcgatgggac gaataccgcg ctggcgcgta gccgtcaccg taaatcgagt tcgcagtttc
     5821 tgaatggtgg caaaatggcg cccccgaaga gattcgaact cctgacccct agattcgtag
     5881 tctgggccgg accgttgaaa tcattgaggt ccgttaccgt aataagaccc tctctaaaac
     5941 ctggaaacaa atgctggagg gcgataattg ccagcgtcgt taccttagcg ggtgacatca
     6001 ataaccatca ggatcggtcg acgagtggca gcttcgcgcc acttcac
//
LOCUS       DATMGX010000365         4272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_164915, whole genome shotgun sequence.
ACCESSION   DATMGX010000365 DATMGX010000000
VERSION     DATMGX010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164915"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..538)
                     /locus_tag="VNR65_05835"
     CDS             complement(206..538)
                     /locus_tag="VNR65_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18232.1"
                     /translation="MKALVRTLFATAALSLAAAGTITPATAAEMDRNQPKAGEQGPPG
                     TVGAMQNEVGNKATSPEDVKRQTEGKPTTAQSAKKGDRDASHPGMTNNPSGTVGAAPG
                     TNPDTTRR"
     gene            complement(658..1068)
                     /locus_tag="VNR65_05840"
     CDS             complement(658..1068)
                     /locus_tag="VNR65_05840"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HWJ18233.1"
                     /translation="MPKITPHEALIYVMITTAAADRQISDRELDRIAQTVRHLPVFMG
                     YDVDNLAKAAERCGDLLSDEEGLDDILELIAGALPKKLYETAYALAVEIAAADLKVPE
                     EEVRLLELLRDALNLDKLVTAAIERAARARHQTL"
     gene            complement(1207..1980)
                     /locus_tag="VNR65_05845"
     CDS             complement(1207..1980)
                     /locus_tag="VNR65_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWJ18234.1"
                     /translation="MTTATITLIGGPTAFVELDGFRLLTDPTFDDPGEYTLPHVTLKK
                     TAKPAFGPDQIGSVDAVLLSHDQHSDNLDTSGRAFLQKADRIITTEAGANRLGGNAEG
                     LRPWTTAEISNGNGGALDITATPARHGPIGIEPLSGDVIGFAVSSKTRAFKSLYITGD
                     TVWFDGVAEVQQRFEPGLVLLFAGAAQTRGTFNLTMNANDAIETAAAFKGAAIVPIHT
                     DGWAHFTESGDDLEKAFRTLGHGERFRRLRPGVPAVFEL"
     gene            complement(2075..3661)
                     /locus_tag="VNR65_05850"
     CDS             complement(2075..3661)
                     /locus_tag="VNR65_05850"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWJ18235.1"
                     /translation="MFGSPAKVKRSKHPKSLSLFAASIGILAAFAPSAFAGAGASELR
                     NAATTPARAVEIAQTAALPSFSALVERIAPAVVSVHVKANPMAIAAHDEEGEDQQSEK
                     DGPFDIAPSQRSGPKGFPPHTPFGHQPPSGPQARQDREKGADSVQPVQGQGSGFFISS
                     DGYIVTNNHVVEGAIKVEVVRDDGDILEAKVVGTDPGMDLALLKVDGGGSFPAVALGH
                     AEAKVGDWVIALGNPFGLEGTVTAGIVSALGRDIGMGAYDSFIQIDASVNRGNSGGPT
                     FNQAGEVIGVNTAIFSPTGGSVGIAFAIPATMVENVVTQLREKGHVTRGWLGVQVQPV
                     TPEIADGLGLKKARGALVSVPAPGGPADKAGIRRGDVILKINDGDVKDGRDMARRIAS
                     IAPSTRVKLTLFRDDKPKTVEVKVAELQDGENGMAPGRGLPQAEIGSLGLSVAPAMEG
                     PDSPGGLAIVSVRPDGKAADAGLVRGDILLTANGTDLLSPDALEKALRTAKAAGKKHA
                     IALVQRSGTQVYVALATDKS"
BASE COUNT          837 a         1220 c         1278 g          937 t
ORIGIN      
        1 ataaagccgg accaggacgt gatcttgggg ccgacggttg gcggaaagag cggcaccgac
       61 gcgagagtca gttgccgcaa ggccaacgca taaagcagcc ttttcttttt tttcagctgg
      121 cggagtgttc agctggttga acctcttggg ctgcccggtt tgatcatcag cgtgcggcct
      181 tactcaaccg ctacacatgt ctcacttacc tacgcgttgt gtcgggattc gttccaggtg
      241 ctgccccgac cgtcccggac gggttatttg tcattcccgg gtgggaagcg tcacggtctc
      301 cctttttggc cgattgagcc gtggttggct tgccttcggt ctgccgtttc acatcctcag
      361 ggctcgtcgc cttgttccca acctcgttct gcatcgcacc cacggttccg ggcggccctt
      421 gttcgccggc cttgggctgg tttcggtcca tttccgccgc ggtcgcaggc gtaattgttc
      481 ccgcagcggc caagctcaaa gctgccgtcg caaaaagcgt cctcacgaga gccttcatcg
      541 tctcctcctt gtttcttagg aaggagaacg ttgctgagca cgctcatgtt cctgatgatg
      601 atagactcgc aggggcgtcg cacgccgcca agcaacgaat tcggatgtgc ccaagaccta
      661 aagcgtctgg tggcgggctc tggcggcgcg ctcgatggcg gccgtaacga gcttgtcgag
      721 gttaagcgca tcgcgaagaa gctcgagaag ccttacttcc tcctccggca ctttgagatc
      781 cgcagccgcg atctcaacgg ccaaggcata ggccgtctcg tacagttttt ttggcaacgc
      841 gccagcaatc aactcaagaa tgtcgtcgag gccttcctca tcgctgagaa ggtcgccgca
      901 ccgttccgct gctttcgcaa gattgtccac gtcatagccc atgaagacgg gcaggtgccg
      961 cacagtctgg gcgatacggt caagttcgcg atccgaaatc tgacggtcgg ccgccgccgt
     1021 ggtgatcatg acatagataa gcgcttcgtg aggcgtgatc ttcggcatcg tgcggtccca
     1081 tggcagtttt gctaaggttt agttaagctc ccgcacgcga cggcgcaagc acccatctca
     1141 aggggaacaa cgccgccgac gacttggctt gcgccgaagg tcccgctgcg gcgtcttctg
     1201 caaattctac agctcgaaaa ctgccggaac accgggccga agccggcgaa agcgctctcc
     1261 atggccaagc gtccggaacg ccttttccag atcgtccccg ctctcggtga aatgcgccca
     1321 tccgtcggtg tggatcggga caatcgctgc gcctttgaag gcagcggcgg tctcgattgc
     1381 gtcgttcgcg ttcatggtaa gattgaacgt gccccgcgtt tgagccgctc cggcgaagag
     1441 caggacaaga ccgggttcga accgctgctg tacttcagcc acgccatcga accaaaccgt
     1501 gtcgccggtg atgtagagcg atttgaatgc gcgggtcttc gaagaaaccg cgaagcctat
     1561 gacgtcgccg gaaagaggtt cgattccaat cgggccatgc cgggctggcg ttgccgtgat
     1621 atcgagggcg ccgccgtttc cgtttgaaat ctccgctgtc gtccaaggcc gaagaccttc
     1681 tgcgtttcct ccaagccggt ttgctcccgc ttcggtggta atgatccggt ccgccttctg
     1741 aaggaaggcg cgtcccgatg tgtcgagatt gtcggagtgt tggtcgtggc tcaggaggac
     1801 ggcatcgacg gacccgatct ggtcagggcc aaatgcgggt tttgccgtct tcttaagagt
     1861 cacatgcggg agggtatact caccaggatc atcgaatgtg ggatcggtca gaaggcggaa
     1921 gccgtcaagc tcaacgaacg cggtagggcc tccaatcagg gtaatcgtag ctgtggtcat
     1981 ggcctctcct tttgcgtgca gagccatcgc gcaaaaagaa accccgccgg actgacgagg
     2041 tttcctttca atgttctttt aaggagaggc tgactcagct cttgtcggtt gccagcgcca
     2101 cgtaaacctg ggtgccactg cgctgcacga gagcgatggc gtgctttttg cctgcggctt
     2161 ttgcggtacg gagcgctttc tcaagagcgt cggggctaag aaggtccgtg ccgttcgctg
     2221 tgagaagaat gtcgccgcga accaagcccg catctgccgc cttgccgtcg ggcctgacgc
     2281 tgacgatggc gagaccgcct gggctgtccg gcccttccat ggctggcgcg acgctcaggc
     2341 cgagcgatcc gatctcagcc tgcggaaggc ctctgccggg agccattccg ttttcgccgt
     2401 cctggagttc ggcgaccttg acctcgactg ttttgggttt gtcatcccga aatagagtca
     2461 gcttcaccct tgtcgagggc gcgatcgagg caatcctccg ggccatgtca cggccgtctt
     2521 tcacgtctcc atcgttgatc tttagaatga cgtcgccacg ccgtatcccg gctttgtcgg
     2581 cagggcctcc gggcgccggg accgagacga gcgcaccgcg tgccttcttc aatcccagac
     2641 catcggcaat ttctggggtg acgggctgga cctgaacgcc gagccatccg cgggtaacgt
     2701 ggcccttttc cctgagttgg gtgacgacgt tttccaccat cgtggccggg atggcaaagg
     2761 cgatgccgac cgagccgcct gttggggaga agatagcggt gttaacgccg atgacttctc
     2821 cggcctggtt gaatgtaggg ccgcccgagt tgccgcgatt tacggaagcg tcaatttgaa
     2881 tgaagctgtc gtaggcgccc atgccgatgt cgcgcccaag ggcggagacg atacccgccg
     2941 tgacggttcc ttcaaggccg aaaggattgc cgagtgcaat cacccaatcg ccaaccttgg
     3001 cctcggcatg acccagcgcg acagcgggaa atgagcctcc gccgtcaacc ttgagaagcg
     3061 caaggtccat cccgggatcg gttcccacaa ccttggcttc gagaatatct ccgtcgtcgc
     3121 gcacgacctc aaccttgatc gcgccttcca ccacatgatt gttggtgacg atgtaaccat
     3181 cggagctgat gaaaaagccg gagccttgcc cttgaacagg ctgcacggag tcggcccctt
     3241 tctcacggtc ctggcgcgct tgcgggccag agggtggctg atgaccaaaa ggcgtgtggg
     3301 gcggaaagcc tttgggaccg gagcgttgcg aaggcgcgat gtcgaacggg ccgtccttct
     3361 ccgattgctg gtcttcgcct tcttcatcgt gggcggcgat tgccatggga ttcgctttta
     3421 cgtggacgga gaccacggct ggtgcgatcc gctcgacgag agcggagaag cttggaagag
     3481 cagccgtttg ggcaatctca acggcccggg cgggcgtcgt ggccgcattt cgaagctcgg
     3541 acgctcccgc tccagcgaag gcggatgggg cgaacgcggc gagaattcca atgcttgcag
     3601 caaagagtga gagggatttt gggtgtttcg agcgctttac cttggcaggc gaaccgaaca
     3661 tgagccgtcc atctggatcg aggagagata cgacagtttc ttttttctta agattgaggc
     3721 aaaaatacat cagtcgtgtc tatcacaaaa caaaaaacga taaagctgag ttaaacgagc
     3781 gacgcaaata acgcaatttt agataaacgc tgtctttgtt gggagttatg cgtgataccg
     3841 ctttccaacg aatctaatta gaaatcagtc aagtacttca atgacatctt ttgagtattt
     3901 tctcgtaagt aaaacagtgt gctgacgaag tgcatcccaa acccgccagc tttgccttcg
     3961 ccgggagacg cgatggaagc gacaccgtgt cgcttccatc gctgatggac ttgcatcgag
     4021 cttgtccgtt ttctcaacgc gatattgcag aagaacgggc tgccacagtc aaagacaaat
     4081 atgacggggt ctgactgccc gaagagcttg attatgagtt cgtgtcagcc ttatcgagag
     4141 tgtacgcata agcggaggat ttcatgaaaa aaatcatcgt cgccgggctg accgcactcg
     4201 cgctgttaag cccttcgttg agttttgccg aggaaacgcc aggcccgcgg ggcgcggctg
     4261 agcaggagca at
//
LOCUS       DATMGX010000366         3714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_73565, whole genome shotgun sequence.
ACCESSION   DATMGX010000366 DATMGX010000000
VERSION     DATMGX010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73565"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VNR65_05855"
     CDS             <1..192
                     /locus_tag="VNR65_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18236.1"
                     /translation="PACEISRERRGLVEGHADPLALAPHDETGNVRSVRLKNKVETFG
                     DVEGLGNIERGPRNGHVAD"
     gene            complement(293..574)
                     /locus_tag="VNR65_05860"
     CDS             complement(293..574)
                     /locus_tag="VNR65_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18237.1"
                     /translation="MQLLASRGYPVIDYQTMGEFIGHERLFRTTDGAFLLHMSSEGKR
                     EEEERIVWLTVRDALSWLNEVPDEFGSFWDCAIDAPLEQKQIHHTGPTF"
     gene            complement(950..1192)
                     /locus_tag="VNR65_05865"
     CDS             complement(950..1192)
                     /locus_tag="VNR65_05865"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWJ18238.1"
                     /translation="MVETRIAPRHRVSKAATIEFVGGRPISCVVRNLSVTGAAIELSN
                     RDGIPDSFLLVMPDDGLRLLCQIVWRKDYRIGVTFD"
     gene            1570..1893
                     /locus_tag="VNR65_05870"
     CDS             1570..1893
                     /locus_tag="VNR65_05870"
                     /inference="COORDINATES: protein motif:HMM:NF027832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrlR family regulatory protein"
                     /protein_id="HWJ18239.1"
                     /translation="MLVNGKYSAWFKIPLGEGTGIVVLEDGNVSGGDTVIAYSGCYKQ
                     DGDAFSADIAIKRHTPGQLSVFGIDDVDISLTGKSTGTVASCRGKSRQAPGMTFEATM
                     IRMAD"
     gene            2093..2320
                     /locus_tag="VNR65_05875"
     CDS             2093..2320
                     /locus_tag="VNR65_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18240.1"
                     /translation="MRRSDPDQHKIADVQWKSGTLVRVNSIDLVMTVVGYDSLGSVIC
                     AWNREEHPVHGYVTSAVLTLADNPETAKTSS"
     gene            2618..2995
                     /locus_tag="VNR65_05880"
     CDS             2618..2995
                     /locus_tag="VNR65_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18241.1"
                     /translation="MKVEFTCPLDFFAFFFATFLPDFFFASPVPGFRALAALFFADDF
                     LTVYFADFLLAFLATFFADVFGAFFEVDFFRRGAATMASRHQRPSRLQRSASRQRLFT
                     RFLGLCLHRVLQSFGGSGSLCLR"
     gene            complement(3502..>3714)
                     /locus_tag="VNR65_05885"
     CDS             complement(3502..>3714)
                     /locus_tag="VNR65_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18242.1"
                     /translation="TRDSRIDPSQCRLIVSGEHNDSKKTGRIVMSNAQEAFAMTAVPF
                     GLSFAGIFLFWFAEKFRPVRGPTIRR"
BASE COUNT          886 a          991 c          972 g          865 t
ORIGIN      
        1 ccagcctgcg aaatttcacg cgaacgtcgg ggattagtcg aaggtcacgc cgatccgctc
       61 gcgttggcgc cacacgacga gacagggaat gtgcgttccg tccgccttaa aaacaaggtt
      121 gaaacgttcg gggatgtcga ggggctcggc aacatcgagc gcggcccccg aaatggtcat
      181 gtcgcggact agcaattaat cgcgcagcca gcgaactgaa tcgcagcgga catcaataca
      241 ggcttgcgag tgatcgcgcg tctttccatg aaaccttgat gtgttgagat tcttaaaaag
      301 ttgggcctgt gtgatgaatt tgcttctgct caagaggagc atcgattgcg caatcccaaa
      361 aagaaccgaa ttcgtccggc acttcattga gccatgatag agcgtcgcgc acggtcagcc
      421 agacgatccg ttcctcctct tcgcgcttcc cttccgatga catgtggagc aggaaggcgc
      481 cgtcagtcgt tcgaaaaagc cgctcgtgtc cgatgaattc gcccatcgtc tgatagtcga
      541 tcacgggata gccacgggat gccaataact gcatgaagat tcggggccca gaattgctcg
      601 ggccaatgag aagctaatat ggttaacggc ccgtaattct accgagacgg gatgggagcg
      661 ctgtacgggc ttctcgattt atcgtgtaaa actcgctcag aatgtcgcag ataggtcatt
      721 cgtgtcgctt ttgacgtatc ctttacttgt ctgctttccc tgcacttcgc tctcgaagcc
      781 attttcgtgt tgtggaatga aaagttcttc gatccgattg atctgtccgg cgggtacaag
      841 ctggccaccg ctccgcaaga ccaagctttg catcgcgcag gggcttcatg cttgggtcgt
      901 cctgcagaag caaaagcctc gttgggagcg aaatccaatt gaagcggcct caatcgaacg
      961 tcacgccgat ccggtagtcc tttcgccaca cgatttggca gagcaggcgc aatccatcgt
     1021 ccggcataac taacaagaaa ctatcgggga taccgtctcg gttagacagc tctatcgctg
     1081 ctccggtaac ggacaggttg cggaccacgc aactgatcgg tctgccgccg acgaactcga
     1141 tggtggcagc tttagagacg cggtggcgag gcgcgatcct ggtttcgacc atagggtcaa
     1201 agagtacgcc ggatttctga gtaatcggta aatctctccg tagtactacg gagaacaaat
     1261 taggatttca ttaaccagcg gccgcaaact gaaattacca aattagaaag gtaggaatgg
     1321 aacttaggag aagcgccgtc acatgtggac gcgccaagga cacatgtgaa cagaacggca
     1381 accggggttc ctcgaatgcc ggctttcggc tcgttagcaa ctgcagcacc tatcgctcga
     1441 tcccctcgtc agcgccagcg agggacgaaa aacgcgtaac tatcgcctaa ccacgacaat
     1501 cggcttgctg gaaataatac cgttgcttca cgttgcccat taggaagaag ttcacgcgag
     1561 gcgcgttcga tgcttgtaaa cggaaaatac tctgcgtggt tcaaaatccc ccttggcgag
     1621 ggcacgggca tcgttgttct ggaagatgga aatgtttctg gcggagacac cgttatcgcg
     1681 tatagcggct gctacaagca ggatggcgac gccttcagcg ccgacattgc aatcaagcgg
     1741 cacaccccag ggcaattgtc cgtgttcggg attgatgacg tcgatatctc gctaaccgga
     1801 aaatctactg gcacggtggc gtcgtgccgc ggaaagtcga gacaggcacc cggcatgacg
     1861 tttgaagcga ctatgatccg catggctgat tgagatagcc cgccaacacc ccacgaatgc
     1921 cccattagcc gacatcttgg tgtcggtctg taaaaagcgc caaacagcga agcggtcgtc
     1981 atcggttacc aaatagttcc tggatattgc cttttctcgg tgtgggcact cacccaagtc
     2041 ttagacttcg agcttaaact gctggaaaag acggaagaac agcgaggcgc aggtgcgtag
     2101 atccgatcct gaccagcata aaatagctga cgttcaatgg aaatctggca ctctcgtaag
     2161 ggtcaactcg attgacttgg tgatgacggt cgtcggctat gacagcctcg gctccgtcat
     2221 ctgtgcgtgg aatcgcgaag agcatccggt gcatggctat gtcacctcag cagtcttgac
     2281 tttggcggac aatcccgaga cggccaagac cagctcatga tcgatgaaaa agtaaaaatc
     2341 aaatgcacaa aatgctcaca agtcttccga gagcgggcgc agaacattcg caatgggttt
     2401 cagactaatt gtcagcattg caatcggatg attacgttcg atagcaattc ggaagataga
     2461 aatatccgtc gggcactcat gtgcgccaaa gaagtacgaa tggcgcttga gtcccagccc
     2521 aaaactccaa ttgtgatgca aggcacgctc agcgatcgca atcgatttta gctctcaacc
     2581 atggagaccg tagcgacttc acgttggcgg tgagacagtg aaggttgagt tcacttgccc
     2641 cctcgacttc ttcgccttct tcttcgcaac cttcttgccg gactttttct ttgccagtcc
     2701 ggttccaggt ttccgcgcct tagcagcctt attctttgcc gacgattttc tcaccgtcta
     2761 ttttgctgat ttcttattag cctttttagc cacctttttc gcagatgttt ttggcgcttt
     2821 cttcgaagtc gacttctttc ggcgaggagc tgccacaatg gcatcgcggc atcagcgacc
     2881 aagccgtctc cagcgatcgg catcaaggca acggttgttc acccgctttc tcgggctctg
     2941 cctccatcgc gtgctgcaga gcttcggagg cagtggttcg ctatgtcttc gatagcgtcg
     3001 ttaatctgat cgaccaactt ctcgactaaa ttcttgtcct tgcatcgtcc atgctgctcc
     3061 ctccaacaat tgccagtcgg gggatattct tatttgagaa tagggcaatc gcttaccgcg
     3121 ctggcttgat ttggatcact gcagccgcgc ttcgcttgtg acacttttcg ataccttcac
     3181 ccacagcggt catcatggcc agacatacag ttctcatcaa tcgctggttc gtctcggtcg
     3241 atacgccgaa acaacaggga cctgtttcct cgcgggcacc ttctgttaga cagacaaaat
     3301 cattcccgac ggagagcgag gccaggcaat ttgcgagggt gaagctttct gaaggaatga
     3361 aggtcacagc cggtacgttg agtcctcacc agcccatacg acgcaacatc acagcctcgg
     3421 aaattaatcg atggatcgag gaacaggtat gatgaccaac cgtcacggtg agctccgcga
     3481 ggtcactcgg ccaagcgaga actaacgtcg aattgttggc cctcgcacag gcctaaactt
     3541 ctcggcaaac caaaataaga aaatcccggc gaacgatagg ccgaaaggca cggcggtcat
     3601 tgcaaaagct tcctgagcat ttgacatgac gatcctccca gtcttttttg aatcgttatg
     3661 ttcaccggaa acgattaagc ggcattgcga tggatcaatt cgactgtctc tggt
//
LOCUS       DATMGX010000367         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_295725, whole genome shotgun sequence.
ACCESSION   DATMGX010000367 DATMGX010000000
VERSION     DATMGX010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295725"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VNR65_05890"
     CDS             complement(<1..389)
                     /locus_tag="VNR65_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWJ18243.1"
                     /translation="MEKTAQRGGAKSAISGKKEPEAAESKAAKIPGTENGAAGQGAEV
                     EGALADARHAYRTSTCGALRRADAGKHVRLSGWVHRVRDHGGLLFIDLRDHYGLTQCV
                     VDPSAPAFKAAEQVRAEWVIRVDGDVV"
     gene            509..1750
                     /gene="rnd"
                     /locus_tag="VNR65_05895"
     CDS             509..1750
                     /gene="rnd"
                     /locus_tag="VNR65_05895"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HWJ18244.1"
                     /translation="MAKSSAWTAIFVKDARPSYAPPHNLSYKNTMTELPPVITSSKDL
                     RNACDLLSRQPFVTVDTEFMRQTTYRPKLCLIQMAAPELEYIADPLAGIDLVPFYALM
                     ANESVLKVFHAARQDIEIVFQEAGVVPRPLFDTQVAAMALGHGEAISYGSLVKKILKR
                     NHDKTYQAVDWCARPLGAKQLEYAIGDVTFLRDIYLRLQQRLQETGRESWLEEEVAVL
                     IDPKTYAFDPADAWKRLKLGGKSRQARAIIMELASWRERTAQDENVPRGRVIKDEAIY
                     EVAKHAPRSLDTLSRLRSVREVLKSRGDAILRAVEAALARDPSDVAGAIAHTDLPASA
                     SAAVELLRVLLKAVSAELNIAPRLLASGEDLEKLAQFDEPDVPALRGWRRQLFGEKAL
                     ALRSGQLALGIKNKEVAVFPL"
     gene            complement(1699..1947)
                     /locus_tag="VNR65_05900"
     CDS             complement(1699..1947)
                     /locus_tag="VNR65_05900"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWJ18245.1"
                     /translation="MEIGKTLIVVGAVLIGAGLLWLLGSRLGLGHLPGDIVIERENFR
                     LYLPLGTCLLISVSAAFWVFAAYSGNTATSLFLIPSAS"
BASE COUNT          403 a          641 c          598 g          385 t
ORIGIN      
        1 atcaccacgt cgccatcgac gcgaataacc cattcggcgc gaacctgctc cgccgccttg
       61 aaggctggcg cgctcggatc gacgacgcat tgcgtcaggc cgtaatgatc gcgaaggtcg
      121 ataaaaagca ggccgccatg gtcgcgcacg cgatgcaccc aacccgaaag ccgcacatgc
      181 ttgcccgcat cggctcttct gagcgcgccg caggtcgagg tccggtaagc atgccgagca
      241 tccgcaagcg cgccctccac ttcggcacct tgcccggctg ctccgttctc tgtgcccggt
      301 atttttgcgg cttttgattc ggcggcttcc ggttccttct tgccggaaat ggcggatttc
      361 gcccccccgc gttgagcggt tttttccaca gatcgcatcc tcttggatca aagccggccc
      421 gccagaaaga ttttcatcat gcgagcggcg cagcggcata aaagagacac acttaagtcg
      481 gccctgtcgt catagagcgc gtcaaaactt ggcgaaatcc agcgcatgga cagcgatatt
      541 tgtcaaggac gcgaggccct cctatgcgcc gccccacaac ttgagctata agaacacgat
      601 gaccgaattg ccgcctgtaa taacgtcctc caaagacttg cgaaatgcgt gcgacctgct
      661 cagtcgccag cccttcgtga cggtcgacac cgaattcatg cggcaaacca cttacaggcc
      721 gaagctgtgc cttatccaga tggcggcgcc ggaactggaa tacatcgccg acccgctcgc
      781 cggcatcgac ctggtgccgt tttacgctct gatggccaac gaatcggtgc tcaaggtctt
      841 tcacgctgcc cgccaggata tagaaatcgt gtttcaggag gcgggtgtgg tcccccgtcc
      901 acttttcgac acccaggtcg cggcgatggc gctcggtcac ggcgaagcca tctcctacgg
      961 gtcgctcgtc aaaaagatcc tgaagcgcaa tcacgacaag acctatcagg ccgtcgattg
     1021 gtgcgcgcgg ccactcggcg cgaagcagct cgaatatgcg atcggcgacg tgaccttctt
     1081 gcgggatatt tatctaaggc tccagcaaag gcttcaggaa acggggcgag aatcctggct
     1141 cgaggaggag gtcgcggttc tcatcgatcc gaagacctat gcgttcgatc cggcagacgc
     1201 ctggaagcgg ctgaagctcg gtggaaaatc ccggcaggcg agagccatta tcatggagct
     1261 tgcctcctgg cgcgaacgca ccgcgcagga cgagaatgtc ccccgcgggc gcgtcatcaa
     1321 ggacgaggcc atctatgagg tcgccaagca cgcaccccgt tcgctcgaca cgctgtcgcg
     1381 cctgcgctcg gtccgtgagg ttttgaaatc ccggggcgac gccattcttc gcgccgtgga
     1441 agccgcgctg gcgcgcgatc cttccgatgt cgccggtgcg attgcccata ccgacctgcc
     1501 cgcgtcggcc tccgccgccg tggaactgct gcgcgtcctt ctcaaggccg tctccgcgga
     1561 gctgaacatt gccccgaggc tgttggctag tggggaagac ctcgaaaagc tggcgcagtt
     1621 cgacgagccg gacgtccccg cgcttcgggg ctggcggcgg caactgttcg gcgagaaagc
     1681 gctcgccttg cgatccggtc agctggcgct tgggatcaag aacaaggaag tggccgtgtt
     1741 cccgctatag gcggcgaaga cccagaacgc cgcggatacg ctgataagaa ggcaggttcc
     1801 gagtggcagg tagagccgaa aattctcgcg ctctatgacg atgtcgcccg gcaagtggcc
     1861 aagcccaaga cgcgatccca agagccagag cagccccgcg ccgatcagaa cagcgccaac
     1921 gacgatcaaa gtcttgccga tttccatgtt atgtagcgca tgtggtgagg gtaacggctc
     1981 gcttcgcctg ctttcgccca gtttgcattg acctgttcat ggccttc
//
LOCUS       DATMGX010000368         3366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_313566, whole genome shotgun sequence.
ACCESSION   DATMGX010000368 DATMGX010000000
VERSION     DATMGX010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313566"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..433
                     /locus_tag="VNR65_05905"
                     /pseudo
     CDS             284..433
                     /locus_tag="VNR65_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(445..705)
                     /locus_tag="VNR65_05910"
     CDS             complement(445..705)
                     /locus_tag="VNR65_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18246.1"
                     /translation="MNPFAKIDGLRGDQNLEIGTNRYHGARRSSANTNLNVSLSARDE
                     TLMSAPSISISIIPPCDKRADFSSLLGFGIVTGTKTGGTKIG"
     gene            634..987
                     /gene="tnpB"
                     /locus_tag="VNR65_05915"
     CDS             634..987
                     /gene="tnpB"
                     /locus_tag="VNR65_05915"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HWJ18247.1"
                     /translation="MIAVRSDLKILIATQPVDFRKGIHGLVALVAEALKANPYCGNIF
                     IFRSKRADRLKLIAWDGTGMVLVTKWLEEGSFVFPKIQDGAMVLTGPQLSVLLAGLDW
                     MNVGAKVVKRPLRTG"
     gene            1039..2622
                     /locus_tag="VNR65_05920"
     CDS             1039..2622
                     /locus_tag="VNR65_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWJ18248.1"
                     /translation="MPLRPEDLSSDPAHLTEMLLASEAENARLRATITQLKGMIFGAR
                     SERRVTLVAEQLPLDLDDSTPGSTAKILAPEPANDDQSNKGGSSGKPENKRDRIIGAL
                     PKHLPRVDLLIEPETTSCPCCAGKLHRIGEDVSETLDRIPAVLRVLRTIRPKYACRAC
                     EEAIVQAKAPARLIEGGMVSTALVSHIAVAKYGWHSTLYRQMQILSGQGVLVDRQTLA
                     RWMKRLAWWLKGLYELQLRVMHANPRLFCDETPIRVLEPGRGRSKICQFWAHAVDDRP
                     WNGPAPPAVAYVFAPSRGKKEIANQLTDFSGILQVDAYAAYKAIEKDSRISGKIELAF
                     CLAHARRKFVAVFKTTQSPFAKEVIEKIAVIYGIEAKIRGMEAERRRASRQLESRPVM
                     EALKARLLAVKDGLSRQSPLTTAIDYTLNHWTGLTLFLEDGRLEPDTNIVERSIRPVA
                     LGRKNALFCGDEGGGETWAILASLINTAKLNGLDPETWLTDVLERIVSGATKNDQLHE
                     LLAWNWKAAREAETNKAAA"
     gene            2619..3206
                     /locus_tag="VNR65_05925"
     CDS             2619..3206
                     /locus_tag="VNR65_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HWJ18249.1"
                     /translation="MTHELFSYDEADAILRLDGANDYVGISAIDGLIASVVAGPTAME
                     PSVWLPEVFGKTPLQTPGTPQHRLLRTVLHRHDEVRDILAQRPHAYLPIFMRDQGQIF
                     AEDWTIGFMLGVGMRDKAWTRILTSSFRATLVPIFSVHPIGRQLMPDVPEAEMDKIKA
                     TACDRIGPAVVALHKHCASDQAASRRLAKLRTGRR"
     gene            complement(3186..>3366)
                     /locus_tag="VNR65_05930"
     CDS             complement(3186..>3366)
                     /locus_tag="VNR65_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18250.1"
                     /translation="AHRWGKTKGKIGFHGGKVEIARPRLRSFDGKEQALPSWKVSGAT
                     TPRRAILAFSAAAGS"
BASE COUNT          729 a          913 c          985 g          739 t
ORIGIN      
        1 cgcgcctcgc acccatatct caacctcaat cggtaatggc cccttcgact ctaacgcctc
       61 caaacgctgt tccagttcgg ataacagttt ctcaacatcg ggtagcggtt cttttgtaac
      121 cggtactaca atcccacctt cccaatgtgc gtgtaggatg cttatcgttt ggagcagacg
      181 ataatgctct ggatggcgca tggataatta taatcatcga cgcaaaagcg agggttccaa
      241 taatcatcgt gatgcggagg cgacgagtgg cacggctccg catcggcatt ggagcgccga
      301 ggaacgtgcg cggattgtcg cggaaagttt tgaaccgggc gccaaggttt cggcggtagc
      361 gcgccggcat ggtgtgagtc gaggtgttct gcatgactgg cggaagaaag ccagagcgtc
      421 tcagctttcc aggcctgcgg ctgatcaacc gattttcgtt cctccagttt ttgttccggt
      481 gacgatgccg aaaccaagca gcgaggaaaa gtctgcgcgt ttatcgcatg gcggaatgat
      541 agagatcgaa attgatggtg cactcatcag agtttcatcg cgcgccgaca aagagacgtt
      601 gagatttgtg ttggcggaac tgcggcgggc gccgtgatag cggttcgttc cgatctcaag
      661 attttgatcg ccacgcagcc cgtcgatttt cgcaaaggga ttcatggcct cgtagcgctt
      721 gtggcggaag ctttgaaagc aaatccttat tgcggcaaca ttttcatctt caggtcgaaa
      781 cgggcggatc ggcttaaact gatcgcttgg gatggcacgg gcatggtttt ggtgacgaaa
      841 tggcttgagg aggggagttt tgtttttcca aagatccagg acggcgcgat ggttctgaca
      901 ggtccgcaat tatccgtgtt gttggctgga ctcgactgga tgaacgtcgg cgcgaaggtt
      961 gtgaaaagac cgttgagaac cggctaaatc acttgttttc ggctcgtgct taaggtggtt
     1021 ttgttgtagt cttttgggat gccgctccgc cctgaagatc tctcatccga ccctgcgcat
     1081 ttgaccgaaa tgctgcttgc gtctgaggct gagaacgcgc gtttgcgagc gacgatcacc
     1141 cagctcaagg gcatgatttt tggcgcccgc tcggaaaggc gcgtcaccct cgtcgcggag
     1201 caattgccgc ttgatcttga tgatagcaca ccggggtcca cggctaaaat tctcgcccct
     1261 gaacctgcca atgacgatca aagcaacaaa ggcggctcgt cgggcaagcc ggagaacaaa
     1321 cgcgaccgga tcattggcgc cttgccaaaa catttgccgc gcgtcgatct gctgatcgaa
     1381 ccggaaacca catcatgccc ctgttgcgct ggcaagttgc atcgcattgg cgaggatgtc
     1441 agcgagacgc tcgatagaat tcccgccgtg ctgcgggtgt tgcgcacgat ccgcccgaaa
     1501 tatgcttgcc gcgcctgcga ggaagccatc gtgcaggcaa aagcgccagc gcgtttgatt
     1561 gaaggcggca tggtttcaac cgcgctggtc agtcatatcg ccgtggcgaa atacgggtgg
     1621 cattcaacgc tctaccgtca aatgcaaatc ctttccggtc aaggcgtgct ggtcgaccgg
     1681 cagactctgg cgcggtggat gaagcgtctc gcctggtggc taaaggggct ttatgagctt
     1741 caattgcggg tcatgcacgc aaatccccgg ctgttctgcg acgagacgcc tattcgcgtc
     1801 ctcgagccag gacggggccg ctcgaagatt tgtcagtttt gggcgcatgc ggttgatgac
     1861 cggccatgga atggccccgc gccgccggcc gtggcttatg tctttgcgcc aagccgcggc
     1921 aagaaggaaa tcgccaatca actcaccgat ttctccggca tcctgcaagt cgacgcctat
     1981 gccgcctaca aggcgatcga gaaggacagc cgcatttcgg gcaagatcga actcgccttt
     2041 tgtctggctc acgcaaggcg taaattcgtc gccgttttca agactactca atcgcctttc
     2101 gccaaagaag tgatcgagaa aatcgcggtc atatatggga ttgaggcaaa aatccgaggt
     2161 atggaagccg agcgacggcg ggcttcccgg caattagaat cacggcctgt gatggaagcc
     2221 ttgaaggcgc gattgctggc ggtgaaggat ggcttgtcac gccaatcgcc tttaacaaca
     2281 gcgatcgatt acacgcttaa tcattggacc ggcttgacgc tcttcctgga ggacgggcgg
     2341 cttgagccag acaccaacat agttgagcgt tcaatccgcc ccgttgcact cggcagaaaa
     2401 aatgctcttt tctgtggtga tgaaggaggc ggagaaacct gggcgatctt ggcctcgctc
     2461 atcaacacgg caaaactcaa tgggcttgat ccggagacat ggctcaccga tgtgctggag
     2521 cggatcgtct ccggggccac gaagaacgat cagctgcatg agctcctggc gtggaattgg
     2581 aaagccgcgc gtgaggcgga aaccaacaag gctgcggcat gacgcacgag ctcttcagct
     2641 atgacgaagc cgatgcgatt ttgcgcctgg atggcgcaaa cgattatgtg ggcatcagcg
     2701 cgattgatgg tctgatcgcc tctgtcgtag ccggccccac ggcgatggaa ccatccgtgt
     2761 ggctgccaga ggtctttggg aaaacccctc tgcaaacgcc gggaacgcca cagcaccggc
     2821 tcctgcggac agtcctacat cgccacgacg aagtcaggga tattttagcg cagcgcccgc
     2881 acgcctatct tcccattttc atgcgggatc aagggcaaat cttcgccgag gattggacga
     2941 ttggattcat gcttggggtc ggcatgcgcg acaaagcatg gacaaggatt ctgacttcaa
     3001 gttttagggc tactctcgtg ccaatctttt cggttcatcc catcgggcga caactcatgc
     3061 ccgatgttcc agaggccgaa atggataaga taaaggcaac cgcctgtgat aggattgggc
     3121 ctgccgtcgt tgctcttcac aagcattgtg ccagcgatca ggccgcaagc cgccggctcg
     3181 ccaaactacg aaccggccgc cgctgaaaaa gctaaaattg ctcggcgtgg ggtagtagca
     3241 ccgcttactt tccaactggg aagggcctgc tccttgccgt cgaagctacg aagccgcgga
     3301 cgcgcgatct ccaccttgcc gccgtggaaa ccgatcttgc ccttcgtctt gccccagcga
     3361 tgcgcc
//
LOCUS       DATMGX010000369         2626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_41033, whole genome shotgun sequence.
ACCESSION   DATMGX010000369 DATMGX010000000
VERSION     DATMGX010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41033"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..501)
                     /locus_tag="VNR65_05935"
     CDS             complement(64..501)
                     /locus_tag="VNR65_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18251.1"
                     /translation="MLSLAPFESAASCFDSTIVAARNAMVGAGCRDGGDPARHLRAID
                     DVLRRLKRKAQATICVLAISDLAEHRALAEMCRVRLAAAINLRREASRCIKRGVAPPL
                     PASYPVARRPNTAREWISLWSCSQAWLVAWAIVWDVAWTSDRN"
     gene            complement(693..917)
                     /locus_tag="VNR65_05940"
     CDS             complement(693..917)
                     /locus_tag="VNR65_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18252.1"
                     /translation="MIRNGSWKKELNGCLTTEVGDVWMMVEKVGARFRVVVTRGGHKT
                     QPGKVLASASAADARAAMALAEQVASQVVV"
     gene            complement(1033..1266)
                     /locus_tag="VNR65_05945"
     CDS             complement(1033..1266)
                     /locus_tag="VNR65_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18253.1"
                     /translation="MSYVSNDWLGMPRRVDLGHALAAEAHEQAARMHRLAAQSHARGE
                     HATAREFAQQAVQQACAADRFTQNAQLLTDDAT"
     gene            complement(1458..1619)
                     /locus_tag="VNR65_05950"
     CDS             complement(1458..1619)
                     /locus_tag="VNR65_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18254.1"
                     /translation="MSAILTKRIAAVVERRDFCKSRVASALGLVASVNVNTLFSSFRR
                     IRKILTGNS"
     gene            complement(1820..>2626)
                     /locus_tag="VNR65_05955"
     CDS             complement(1820..>2626)
                     /locus_tag="VNR65_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ18255.1"
                     /translation="AWRQMVRLQAAPWTASEIKAARELRLAIVSIVFRQAEVRANLVD
                     ALAQPLSGAVAFFHGCDLMVRSGDNYSKQGLLEGLRRGLEALGRASAIVHNLRGFLRK
                     DIAKQRKLDINAAVRNAVQIGAAGFERQGKGRIICDLAPALPQLIADGAQIQQAIANL
                     IRNAIEAMAQGGVAVRELRVATALVEGGIEVLVADTGPGLPPEIAQAPFRFFATTKPN
                     GLGLGLSICHSIIESHGGALHFETDAEGTRFRILLPAEPESEQEPGSDAA"
BASE COUNT          518 a          805 c          794 g          509 t
ORIGIN      
        1 ctgatggaag agaggcgatg actgctacat ccagaaccgt cgtggcctgg cggtgtcact
       61 cgatcagttc ctgtcggacg tccacgctac gtcccaaacg atagcccacg ctaccaacca
      121 agcctgactg caggaccaca gcgagatcca ttctcgcgca gtattcggcc gtctggcaac
      181 tggatacgat gccggtagcg gcggcgcgac gccccgcttg atgcagcgtg aagcttctcg
      241 gcggagattg atggcagcgg ccagtcgaac acggcacatc tccgccaacg ctctgtgctc
      301 cgccagatcg gagatcgcga gcacgcagat cgtcgcctgc gccttcctct tgaggcggcg
      361 gagtacatca tcaattgcgc gcagatgacg cgccggatca ccgccatcgc gacagcctgc
      421 cccaaccatg gcgttccgag cagcgactat ggtgctgtcg aaacaggacg ctgcgctttc
      481 gaagggcgca agcgatagca tgggtatctc agaatcagat gacagcggca ttgaagtata
      541 ccgacacttc tggtattgta cagaatggcg gaacgggccg gcggcactgt cagcaacacc
      601 ggaattgcgt tggcgctgag cgccttccgc aacctgccgg gtgcgccatg attggcggca
      661 cccaggcacc gaccacaatc atgacgctgg gatcaaacaa cgacctgtga agcaacctgc
      721 tccgctaggg ccatggcggc gcgggcatcg gctgcactcg ccgaagcaag aaccttcccc
      781 ggctgggttt tgtgtccccc gcgggttacg acaacacgga acctcgctcc cactttctcc
      841 accatcatcc agacgtctcc gacctcggtg gtgaggcagc cgttcagctc ttttttccaa
      901 gacccgttac gaatcacact atcctcccaa atgcctgcgg cgatcagttg cagcggatca
      961 tccctgatgc gtcgcccctt ggtcgaaccg acgctttagc tgacaaagtg gagtttctga
     1021 ttcggacaat tgtcatgttg cgtcgtcagt caaaagttgg gcgttctgcg tgaaacgatc
     1081 ggcggcgcat gcttgctgca cagcttgctg cgcaaactcg cgagccgtcg catgttcgcc
     1141 gcgcgcgtgg ctctgggcag caaggcgatg catcctcgca gcttgctcgt gtgcctcggc
     1201 ggctagcgcg tggccgaggt cgacgcgacg cggcatcccg agccagtcgt tgctaacgta
     1261 gctcatttgt gttgccagtt tcatggtgct ttgctttgga atccggtttc acgcaggctc
     1321 agctcatatg caacgactga cggtgcatgg cgtgagccgt tatccgtcgt ctgtttttga
     1381 cacgcggcaa tggggcattg ccatgttttg atgcgcgctt cctagcgatt aagagcaact
     1441 aggacgaagc cggcgattca ggaatttcct gtcaggattt tcctgatacg gcggaagctg
     1501 gagaagagcg tattgacgtt cacggaggcc acaagaccga gtgcggaagc gacccgagac
     1561 ttgcagaagt cacggcgctc gacgaccgcc gcgatacgct tcgtcaagat tgcgctcatg
     1621 ccgtcgccgc catcgagggc aggctcacgg cgaggatggg cactcggatg gcggccgggg
     1681 cgaagcggcg gcgctcttct gccaggtccg gcatgctgcc atctgccact ggctccgtta
     1741 gcgcggcggc aaagcagcca agcgaatccg ggaaagtctc agtccgtgct ggccgtgctc
     1801 acgtggtctg gggatatgat caagccgcat cggaaccagg ctcttgctcc gattcaggct
     1861 cagccggaag caatatgcga aagcgggtcc cctcggcgtc ggtttcaaag tgcagcgctc
     1921 caccgtggga ctcgataatg gaatggcata tggaaagccc aaggccgagg ccgttgggtt
     1981 tggtggtggc aaaaaaccgg aagggtgcct gggcgatctc gggggggagc cccggcccgg
     2041 tgtcagcgac gagaacctca atcccaccct ccaccagagc cgtcgcaacc cgcaattctc
     2101 gaacggcaac gcctccctgc gccatggcct cgatagcatt tcggataagg ttggcgatgg
     2161 cctgctggat ctgtgcgcca tcggcgatca attgaggtag tgcgggagca agatcgcaaa
     2221 tgatccgacc ttttccctgt cgctcaaagc cagctgcgcc aatctgcacg gcgttgcgga
     2281 cggcggcgtt gatgtccagt tttcgctgct tcgcgatgtc cttgcgcagg aaaccgcgaa
     2341 ggttgtgcac gattgccgaa gcccgtccga gcgcttcgag cccgcggcgc agtccctcaa
     2401 gcaggccttg cttgctatag ttatcgcccg accggaccat caggtcacaa ccatgaaaga
     2461 aggccacagc tccggataga ggttgagcta gcgcgtcgac caggttggcg cgcacttccg
     2521 cctgacgaaa gacaatgctg acgatcgcca agcgcagctc gcgcgcagcc ttgatttctg
     2581 acgccgtcca cggcgccgcc tgcagccgga ccatctgccg ccaggc
//
LOCUS       DATMGX010000370         6284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_29983, whole genome shotgun sequence.
ACCESSION   DATMGX010000370 DATMGX010000000
VERSION     DATMGX010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29983"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VNR65_05960"
     CDS             <1..634
                     /locus_tag="VNR65_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ18256.1"
                     /translation="FSALFRERFEGQPLKGAEFSFGGERVRGEALVTRIGLEGGAIYA
                     LSPQLRDAIEGDGSAILKIDLRPGTSAESLEASLSRPREKQSLANVLRKTAKLAPVAV
                     GLLHEAARQEGNPLACAAPADLAARIKSVPVRLVATAPIERAISTAGGVAFEELDTHF
                     MLRRKPGTFIAGEMLDWEAPTGGYLLQGCFSTGAAAARGALAWLGLSRRD"
     gene            695..832
                     /gene="rpmH"
                     /locus_tag="VNR65_05965"
     CDS             695..832
                     /gene="rpmH"
                     /locus_tag="VNR65_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWJ18257.1"
                     /translation="MGKRTYQPSNLRRKRKHGFRQRMSTKQGQLVLARRRARGRKKLS
                     A"
     gene            859..1257
                     /locus_tag="VNR65_05970"
     CDS             859..1257
                     /locus_tag="VNR65_05970"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HWJ18258.1"
                     /translation="METLKTRVEFKRVQKGRKWVAPGFILQGLARLSEEANGPRFGFA
                     VSSKSVAKESPGGKRRGKAVDRNRARRRLKEAVRLTFAGHAETGFDYVIIGRASALTR
                     NFADLLADMRLAFHKVSQPARSDKDRNPVL"
     gene            1388..3397
                     /gene="yidC"
                     /locus_tag="VNR65_05975"
     CDS             1388..3397
                     /gene="yidC"
                     /locus_tag="VNR65_05975"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWJ18259.1"
                     /translation="MENNKNFILAIALSLAVLIGWQFLYMGPAQKARQQAIEAAQKAE
                     TQTARTETQTANGQVSSPSPAMQVPSTTAAAYVSREAALASSPRVKIDSPALLGSINL
                     KGGRVDDLVLRDYTVSVDPNSAKVDLLSPSGSPHPYFAFTGWLTNEPGVVVPNENTVW
                     EQEGTGPLTAISPVVLRWDNGAGLIFHRKISVDSRYMFMISDEVENKSGNPVTLWPWG
                     QVARLSEPQGLLGYSVLHEGLIGVLGSTGLKELTYANALKNFDPATNMAKESISSDKG
                     GWLGFTDQYWAAVLIPDQSKAYEASMFGNEKKQFYANLRQHPITVQPGQRDGVTNRMF
                     AGAKEVGLLDKYTADYGIDRLNLLVDWGWFAFITEPMFHLMNWFYKLIGNFGVAILLT
                     TVVVKLLFFPLANKSFESMSKMKKLQPELKKIQERFKDDKPRQQQAMMTLYKKEKVNP
                     MAGCLPIVIQIPVFFALYKVLLVSIEMRHAPFFGWIKDLSAPDPTTVFNLFGLIPWTP
                     PQYLMLGALPLVMGISMWVQMRLNPAPPDPMQQKIFAWMPVVFTFMLAHFPAGLVLYW
                     TWNNILSFGQQAFIMKRQGVDIDIMHNVGIKQVGGGVKKAGSKLKSNGKQAVTTERQD
                     ADIDVVQNAGSKKAGGPVKKGGSKLRSNAQPALPPSDRDASGRGD"
     gene            3375..4016
                     /gene="yihA"
                     /locus_tag="VNR65_05980"
     CDS             3375..4016
                     /gene="yihA"
                     /locus_tag="VNR65_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HWJ18260.1"
                     /translation="MHPAEEIEARELAARSLDLFKAPADFVLGVAEVDGLPHSDRVEI
                     AFAGRSNVGKSSLLNALLGRRSLARVSNTPGRTRELNYFSVGDTLYVVDMPGYGYAKA
                     PKSLVKGWTQLIHDYLRGRPQLKRVMLLIDSRHGLKSSDQEIMTMLDKAAVSYQAVLT
                     KADKPKSGDLSQVIRETEEALRKHPAAHPKVLATSAVEGLGIEDLRLAIAELC"
     gene            4263..5060
                     /locus_tag="VNR65_05985"
     CDS             4263..5060
                     /locus_tag="VNR65_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18261.1"
                     /translation="MPKKFALCLICWIAAFQFGLTGASAQSEPADAQFALAPQPAMTL
                     DDPRLLLMIRNAVIALNQANLTGNYSVLRDLGTQNFQMSNNSARLAESFATLRARKLD
                     ISPVMFFNPKFVAPPSMQGGQVLRVTGFFPTTPEQVNFDLAFQLSGEQWMLAAIAVNV
                     TPPGENVQAFAASALQPARPAETGDAKPIRIDLSQSSAPASTQPVTPKKAAPKKPKQP
                     AQKTATAVQPAASPAPAAAPEQQPPAQAPRPPSPPSPGPAAWDPFGR"
     gene            5062..6000
                     /gene="mepA"
                     /locus_tag="VNR65_05990"
     CDS             5062..6000
                     /gene="mepA"
                     /locus_tag="VNR65_05990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF006947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HWJ18262.1"
                     /translation="MKTFILCAAACALLAAFCGRVNAEDAAAFDQQEAKKAFGSVKTP
                     VAQAPQAIGRYERGCVAGAQKIPASGAGWQTMRLSRNRVWGHPILIGYLQRLAGDVRR
                     LDGLPGILVGDMAQPIGGPLQGGHASHQMGLDVDLWYTLMPDHALSANERDHLAADNM
                     VDQAALTTDARKWTEAQIKLLRRAASYAEVARIFVHPAVKKALCEIAGNDRSWLPKIR
                     PWFQHDDHFHVRLDCPPGNPACISQAPVATDDGCGEELDDWFKKLRAPPKPPNKRTIQ
                     PAKPKPMLVADLPKECQALLLAAEGAPKQKGAWAPN"
BASE COUNT         1340 a         1864 c         1830 g         1250 t
ORIGIN      
        1 cttttccgcc ctctttcgcg agcgtttcga aggacagccg ttgaaaggag ccgagttcag
       61 tttcggcggc gaaagggtcc gcggcgaagc gctcgtgacc aggatcggcc ttgaaggcgg
      121 agcgatctat gccctgtcgc cgcaattgcg tgacgccatc gagggcgacg gcagcgcgat
      181 cctcaaaatc gatctgcggc ccggaaccag cgcggaaagt ctagaagcct cgctttcgag
      241 accgcgcgaa aaacaatcgc ttgcgaacgt cttgcgaaag accgccaaat tggcgcctgt
      301 tgccgtcggc cttttgcatg aagcggcacg gcaagaggga aaccctctag cctgcgcagc
      361 gccagcggac cttgccgcca ggataaaaag cgttcctgtc cggctcgtag ccacggcgcc
      421 catcgagcgc gccatctcga cggctggcgg cgttgccttc gaagaactgg acacccattt
      481 catgttgcgc cgcaagcccg gcacgttcat cgccggagag atgcttgatt gggaagcccc
      541 gacgggcggc tatctcctgc aaggctgctt ctcgaccggc gctgccgccg caaggggcgc
      601 tctggcgtgg cttggcttga gccgtagaga ttgacggagc cttaataacc gccctatcta
      661 tcagcctgag aacgaattag gaaagtacgc cccgatggga aagagaacat accagccgag
      721 caaccttcgc cgaaagcgca agcatggttt tcgccagcgc atgtccacga agcaggggca
      781 gctcgtcctt gcacggcgcc gcgcccgtgg gcgtaagaag ctatcggcgt aggccaggac
      841 caacccgcgc gacacgccat ggagacactc aagacgcgcg tagagtttaa gcgcgtgcaa
      901 aagggccgca agtgggtcgc tccagggttt atcctgcagg gccttgcgcg gctgtccgag
      961 gaagccaatg gcccgcgatt tggctttgcc gtttcgagca agtctgtcgc aaaggaaagc
     1021 ccgggcggca aaagaagagg aaaggccgtt gaccgcaatc gtgcgcggcg aaggctgaag
     1081 gaggctgtgc ggcttacatt tgccggccat gctgagactg gattcgacta cgtgatcatt
     1141 ggccgcgcct ctgccttgac tagaaatttc gcggacctac ttgcggacat gcggctcgcg
     1201 ttccataaag tgagccaacc ggcgcgctcc gataaggatc gcaaccctgt cttgtgatcg
     1261 atgtgccggt tttgccggtt ctgcttccct agagggcagg aaccggctaa agcgattggc
     1321 tgtcgaatta gctgacttgc ttcgcggggc gtgaagacgc acttcgaaga tctcccttga
     1381 gcggtttatg gaaaacaaca aaaacttcat tctcgctatt gcgctttcgc tcgctgtcct
     1441 gatcggctgg caatttcttt acatgggccc agcccaaaaa gcccggcaac aggcgataga
     1501 ggcggcgcaa aaggcggaaa cgcagacggc cagaaccgag actcagaccg cgaacggaca
     1561 ggtgtcatcg ccttcgcccg ccatgcaggt tccctcaacg actgcggcag cttatgtgtc
     1621 gcgcgaagca gcgcttgcat catcgccgcg cgtaaagatc gacagcccag ctctgctcgg
     1681 ctccatcaat ctgaagggcg gccgggtcga tgacctcgtc ttgcgggact atacggtttc
     1741 cgtcgacccg aacagcgcga aagtggatct tttgtcccca tcgggcagtc cgcatcccta
     1801 tttcgccttc acgggctggc tgacgaatga gccgggcgtg gtggtgccga acgaaaatac
     1861 ggtatgggaa caggagggca cgggccctct gacagcgata tcgcctgttg tccttcgctg
     1921 ggataacggt gccggcctga tattccatcg caagatttcg gtcgatagcc gttacatgtt
     1981 catgatctcc gatgaggtcg agaacaaatc gggcaacccg gtcacactct ggccttgggg
     2041 ccaggtggcg cgcttgagcg agccgcaagg acttcttgga tattcggtgc tccatgaggg
     2101 gctgatcggc gtccttggct cgacgggcct caaggaactg acctacgcca acgcgctcaa
     2161 gaactttgat ccggcgacca acatggccaa ggaatccatt tcatccgaca agggcggatg
     2221 gctcggcttc accgaccaat attgggcggc cgttctcatt cccgatcaat cgaaggccta
     2281 cgaagccagt atgttcggaa atgagaagaa gcagttctat gcgaacctgc ggcaacatcc
     2341 gataacggtc cagcccgggc agagggacgg cgttacaaac cggatgttcg ccggcgcgaa
     2401 ggaggtcgga cttctcgata aatatacggc ggactacggc atcgaccgat tgaatctgct
     2461 tgtcgactgg ggatggttcg ccttcatcac cgagccgatg ttccacctga tgaattggtt
     2521 ttataagctc atcggcaatt tcggcgtggc gatcctgctg accaccgtgg tcgtaaagct
     2581 tctcttcttc ccgctcgcga acaagtcgtt cgaatccatg agcaagatga agaagcttca
     2641 accggagctg aagaagatcc aggagcggtt caaggacgac aagcctcgcc aacagcaggc
     2701 gatgatgacg ctgtacaaga aggaaaaggt taacccgatg gccgggtgcc ttccgattgt
     2761 gatccagatc cccgtattct tcgcacttta caaggtgctg ctcgtctcga tcgagatgcg
     2821 acatgcgccg tttttcggat ggatcaagga tctctcggcg cctgacccga ccacggtttt
     2881 caacctcttc gggcttatcc cctggacgcc gccgcaatac ctgatgctcg gcgctctgcc
     2941 gctggtgatg ggcatttcga tgtgggttca gatgcggctc aatccggcgc cgccagaccc
     3001 gatgcagcag aagatcttcg cctggatgcc ggtcgtcttt accttcatgc tggcgcattt
     3061 cccggctggg ctggtgctct actggacgtg gaacaacatc ctgtctttcg gacagcaggc
     3121 ttttatcatg aagcgccagg gcgtcgatat cgacatcatg cacaatgtcg gaatcaaaca
     3181 ggtcggcggc ggcgtcaaga aggccggatc gaagcttaag agcaacggca aacaggccgt
     3241 gacaacggaa cgtcaggacg ccgatatcga cgtcgttcaa aatgccggga gcaaaaaggc
     3301 cggcggtcca gtcaaaaagg gtggatcgaa gctgaggagc aatgcccaac ctgcactgcc
     3361 gccgagtgac cgcgatgcat ccggcagagg agattgaagc gcgtgagctt gccgctcgct
     3421 cgcttgatct gttcaaggcg cctgcggact tcgtcctggg cgtggccgaa gtcgatggcc
     3481 tgccccacag cgaccgcgtg gagatcgcct tcgcagggcg gtccaatgtc ggcaagtcga
     3541 gccttctcaa tgccctgctt ggccgcagaa gccttgccag ggtctcgaac accccgggcc
     3601 gcacgcggga acttaactat ttttccgtcg gcgacacgct ctacgtggtc gacatgccag
     3661 gctacggcta cgcgaaagcc ccgaaatccc ttgtgaaggg ctggacacaa ctcatccacg
     3721 attacctgcg cggacgtccc cagctaaagc gcgttatgct ccttatcgac agccgccacg
     3781 gcctgaaaag ttccgatcaa gagatcatga cgatgctcga caaggcagca gtttcctatc
     3841 aggccgtcct cacaaaggcc gacaagccca aatctggtga cctttctcaa gtcattcgcg
     3901 aaaccgagga agctcttcgc aagcatccgg cagcacatcc gaaggtgctc gcaacatccg
     3961 ctgtcgaagg actcggaatc gaggaccttc gcctggcgat cgcggagctc tgctagtgga
     4021 gcttttgatt tcgacatttg ctctgggaaa cggatacaaa tgtcgaaatc ggacgactaa
     4081 atttcgcctt tgcaaacctg caaggagcgg agggcagtct tggcagcttc atgtgtgttc
     4141 ggcggcgcat ctgtgctttc gcgcaatcga ccgttgcata actcagccga cgagacccgt
     4201 ttcgtcttga agtcgccgtt tacggtggct aagagtcgat agttggctag gtggaggaag
     4261 ccgtgcccaa gaaatttgct ctatgcctta tttgctggat tgcagcgttc caattcggtc
     4321 tgacaggggc gtcggctcag tctgaacctg ccgatgcgca attcgcccta gcgccgcagc
     4381 ccgccatgac tctcgacgac ccacgccttc tcctcatgat ccgcaatgcg gtcatagcgc
     4441 tcaatcaagc aaacctgaca ggaaattact ctgtcttgcg ggaccttggc acgcagaatt
     4501 tccagatgtc gaacaactcc gcgcggcttg ctgaatcgtt tgcaacgctt cgcgcgcgaa
     4561 agctcgacat ttccccggtc atgttcttca acccgaaatt tgtagcacct ccgtcaatgc
     4621 aaggggggca ggtccttcgg gtgaccgggt tcttcccaac aacgcctgaa caggtcaatt
     4681 tcgatctcgc ctttcagttg tccggcgaac aatggatgct cgccgcgatt gctgtcaacg
     4741 tcacgcctcc gggcgagaac gtccaagcat tcgctgcgtc agccctccag cctgcccgcc
     4801 cagccgagac cggcgatgcg aagcccatcc ggattgacct gagtcaatct tctgctccgg
     4861 cctccactca accagttacc ccaaagaagg ccgcgccgaa gaagccgaag cagcccgcgc
     4921 agaaaaccgc cacggcggtc caaccagcag cttcaccggc tcccgcggcc gctcccgagc
     4981 agcaaccacc agcgcaggcg ccgagaccgc cctcaccacc gagccccggc ccggcggcat
     5041 gggacccctt tgggcgataa cgtgaagact ttcatccttt gcgccgccgc ctgcgcgctc
     5101 ctggctgcct tttgcgggag ggtgaacgct gaggatgccg cagcgttcga tcagcaggaa
     5161 gcgaagaagg cgttcggctc ggtgaaaacc cccgttgcgc aggcgccgca ggcgattgga
     5221 cgctatgagc gcggttgcgt cgcgggtgcg caaaaaattc ccgccagcgg cgccgggtgg
     5281 cagacgatgc gtctctcgcg caatcgcgtt tggggccatc ccatcctgat agggtacctc
     5341 caaaggctcg cgggcgacgt gaggcggctg gacggcttgc cgggcattct cgtaggcgat
     5401 atggcccagc caatcggagg ccctttgcaa ggtggtcacg cgagccacca aatgggcctc
     5461 gatgtggacc tttggtacac gctgatgccc gatcatgcgc tctcggctaa tgagcgcgac
     5521 catcttgccg cggacaacat ggtggatcaa gccgccttga cgaccgatgc aaggaaatgg
     5581 acggaggcac aaatcaagct tctccggcgc gcagcctcct acgcagaggt cgctcgtatc
     5641 ttcgtgcatc ccgccgtcaa aaaggctctt tgcgaaatcg ccgggaacga tcgctcatgg
     5701 ctgccgaaaa tccgcccctg gttccaacac gacgatcatt tccacgtgcg cctggattgt
     5761 cctccaggaa accccgcttg catctcccag gcgcctgtcg ctacggacga tggctgcggc
     5821 gaagaattgg atgactggtt caaaaaattg agagcgcctc ctaagccgcc gaacaagaga
     5881 acgatccagc ctgcaaagcc aaagccgatg cttgtcgcag acctccccaa ggagtgccag
     5941 gctttgctgc tcgccgcaga aggcgctccg aaacagaaag gcgcctgggc accaaattga
     6001 cgagcgtgga gacttgagcg tcagttgtac ctcttgtacg tgaggatgct gccctcaagc
     6061 ggaaagtgag agctttcgct tgtgatcccc ggcaaggaag gagagcagca gcggagctta
     6121 ttccaggcgc gtcagcctgt gagagttaac gatcggctaa aattggtttt gttttgtaaa
     6181 gtccctataa gtaaacatgt aagtattttt ttctctttgc aaaggcgtcg tgacttagcg
     6241 tagccctttc catttcgacg cggatgcgct gctctggcgc ttta
//
LOCUS       DATMGX010000371         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_98885, whole genome shotgun sequence.
ACCESSION   DATMGX010000371 DATMGX010000000
VERSION     DATMGX010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98885"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VNR65_05995"
     CDS             complement(<1..189)
                     /locus_tag="VNR65_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWJ18263.1"
                     /translation="MKADLSAASVHYANLNSAKLRGANLIEADLYRAHLNWADLRGAN
                     FTGANLREATLDSSFLAGA"
     gene            complement(204..659)
                     /locus_tag="VNR65_06000"
     CDS             complement(204..659)
                     /locus_tag="VNR65_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWJ18264.1"
                     /translation="MSAQLKPYEGGDGEFDFVSVLLRGVDVWNPWRIANRGLSIILSG
                     ADLGEADLRGADLSGADLRWIYLGRANLSGASFRGAYLNEAILCETNLNKADLREANL
                     EQVDLEKAYLIGAKLGGSNLSGAHLRWANLKEAVLPGPFSSRPTSSVPF"
     gene            1173..>2367
                     /locus_tag="VNR65_06005"
     CDS             1173..>2367
                     /locus_tag="VNR65_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ18265.1"
                     /translation="MIGFIFFALAFAAMFVLAIRREPLSMWALWAAVVAFLAQFGLFS
                     GNLHGPDMSAIDVIAFLPAIVFGLLSLRPIRRAVVTRPAFKTVQRVLPPVSKTEQEAL
                     DAGTQGFDAELFTGRPNWSKLRAVNPITLTDEEQRFLDNETEELCKKLNDWEIRHNRN
                     GIPEDILQFIRDKGFLGMLISKDHGGLGFSPQAQSMILGKVSSRNPDASIVVMVPNSL
                     GPGELIEQFGTDEQKKRYLEPLAKGREIPCFALTSPVSGSDAAAMRDVGTVTTGTHEG
                     KEVLGIRLNFDKRYITLAPKATIVGLAFHLLDPDNKLGRGQDIGITLALIPANHPGVE
                     IGRRHLPAGSSFPNGPVRGENVFIPIEWVVGGKDRVGQGWRMLMACLAAGRAISLPAA
                     ATAGAK"
BASE COUNT          483 a          678 c          678 g          528 t
ORIGIN      
        1 ggctccagcc agaaaggagg agtctaatgt agcctctcta agattagcac ctgtgaaatt
       61 ggctccgcgg agatcagccc aattgagatg cgccctataa agatcggctt ctatgagatt
      121 ggcaccgcgc agcttggccg agttaagatt ggcgtagtga acgctggcgg cactgaggtc
      181 tgctttcatg agatcggcgc cgctcagaat ggcaccgatg aggtcggcct tgacgagaat
      241 ggccctggga gaacggcctc tttcaggttg gcccacctga gatgtgctcc gctaagatta
      301 gaccctccaa gcttggcccc aatgagatag gccttctcga gatccacctg ttcaagattg
      361 gcttctctga ggtcagcctt gttcagattg gtttcgcaga gaatagcctc gtttaggtat
      421 gctccgcgga aactggcacc gctgagattg gcccgaccaa gatagatcca tctgaggtcg
      481 gctccgctca gatcggctcc tcggagatca gcttctccaa gatcagcccc gctgagaatt
      541 atcgagagtc ctctatttgc tatcctccaa gggttccaga catccacgcc acgcaagagg
      601 acactgacga agtcaaactc accgtcgcca ccctcataag gcttaagctg tgcggacatt
      661 tccggttcct tggagtggcg atgggacgct cacgcgatct aagtgttttg agagtaccca
      721 acttttttga attgtttcct aagcgtgatg acattcattc ctaaaaacag gaaagcactc
      781 tgatttcccg tgcaagtcgc aaccgcttag cggcgtagca ggcggtcgcc ccgacattgc
      841 ctcacttgaa agtcatgacg tatacgtcaa ctgcctcttg ttaacgacaa gatgatgggt
      901 taggttttgc atgcgatggc agagaaaaat gagatgcctc tatagatgcc cagcgagatc
      961 tgcacatcta tggttagtgc gctctaaacc tttttctgcg aaagctccgt gagagctaaa
     1021 catccgtttg agcgctgccc acgccgcgca ttgagatttg agccggcgct aactacgtgc
     1081 agatcatttg atgccgtctt ttcaggcggc tccgtgcgag taagcagatt gcgataccgg
     1141 ctcggccggt ctgaccaaga agggaagcca ccatgatagg ctttatattc ttcgcactgg
     1201 cgtttgccgc gatgtttgtg ctggcgatca gacgcgaacc gctctcgatg tgggctttgt
     1261 gggccgccgt ggtggcgttc ctcgcgcagt tcggcctttt cagcggtaac cttcacggcc
     1321 ccgatatgtc ggctatcgac gtgatcgcct ttcttccggc gatcgtgttt ggcctgttat
     1381 cgcttcggcc catccggcgc gcggtcgtca cacgcccggc tttcaaaacg gttcagcggg
     1441 tccttccgcc ggtttcgaaa accgagcagg aagctctcga cgctggcacg cagggcttcg
     1501 acgcggagct tttcaccggc cgtccgaact ggagcaagct gcgcgcggtc aatccgatca
     1561 ctttgacgga tgaggaacag cgctttctcg acaatgaaac cgaggagctg tgcaagaagc
     1621 tgaacgactg ggaaatccgc cacaaccgga acggcatccc cgaggacatc ctgcaattca
     1681 tccgcgacaa gggcttcctc ggaatgttga tttccaagga ccatggcggg ctcggttttt
     1741 cgccgcaggc ccagtcgatg atcctgggca aggtttcctc gcgcaaccct gacgcctcaa
     1801 tcgttgtcat ggtgccgaac tcgcttggcc cgggcgaact gatcgagcag ttcggcacag
     1861 acgagcaaaa gaagcgctat ctggagcctc ttgcgaaggg cagggagatc ccctgtttcg
     1921 ctctgacaag ccccgtttcc ggttcggatg ccgccgccat gcgcgacgtg ggcactgtga
     1981 ccacgggcac tcacgagggc aaggaagtcc tcggtatccg gctcaatttc gataagcgct
     2041 acataacgct tgctcccaag gcgaccatcg tcggcctggc gtttcatctc ttagatcccg
     2101 acaacaagct tgggcgcggc caggatatcg gcatcacgct ggcgcttatt ccggccaacc
     2161 accccggcgt ggaaatcggg cggcggcatc tgcccgcagg gtcgtcattc cccaacgggc
     2221 ctgtgcgcgg cgaaaacgtg tttatcccga tcgagtgggt tgtcggcggt aaggaccggg
     2281 tgggtcaggg ctggcgcatg ctaatggcgt gccttgcggc tggccgcgcg atctcgcttc
     2341 ctgcggcagc cactgccggc gcgaagg
//
LOCUS       DATMGX010000372         2571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_411354, whole genome shotgun sequence.
ACCESSION   DATMGX010000372 DATMGX010000000
VERSION     DATMGX010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411354"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VNR65_06010"
     CDS             complement(<1..335)
                     /locus_tag="VNR65_06010"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWJ18266.1"
                     /translation="MVGKDGKRSGGGGDSGRVPILPLPLPSNVARLPSADRREKLRIL
                     EALLFAASEPLDEASLAKHLGAKDDVFQLLDELQNVYAGRGVNLVRVAGKWAFRTAED
                     LSFLLEHYAV"
     gene            865..1134
                     /locus_tag="VNR65_06015"
     CDS             865..1134
                     /locus_tag="VNR65_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWJ18267.1"
                     /translation="MSQQHEIEDDLDAAYVSRVRPHIERLAQAEEDVVLDFSKVEFVD
                     SSGLGALVFLHKRLVSKGHKLRVVGLRGQPLQLFTNLHLVPLFCD"
     gene            complement(1160..2057)
                     /locus_tag="VNR65_06020"
                     /pseudo
     CDS             complement(1160..2057)
                     /locus_tag="VNR65_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031384830.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            2373..2448
                     /locus_tag="VNR65_06025"
     tRNA            2373..2448
                     /locus_tag="VNR65_06025"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2406..2408,aa:Lys,seq:ttt)
BASE COUNT          583 a          731 c          681 g          576 t
ORIGIN      
        1 accgcataat gctcgaggag gaaggagaga tcctccgccg ttctgaaagc ccactttccc
       61 gccacccgga ccagattgac gcctcgcccg gcataaacat tctgcagttc atccaaaagc
      121 tggaagacgt cgtccttggc gccaagatgc ttggcgagcg aagcctcgtc gaggggctcc
      181 gacgcggcaa acaaaagcgc ctccagaatt ctcagcttct cgcgccggtc ggccgaaggc
      241 agccgcgcca catttgacgg taaagggagc ggcaaaatgg gcacgcgccc gctatcgcct
      301 ccgccaccag atcttttgcc gtccttgcct accattcggg aaccttccgc tatccgcagc
      361 ttgttcgaag gctagtgagc gaatctcaca cgcgtccacc agctcgcgct caaatgtcag
      421 attcaaaagc tctactagag ttaataaatt cctatagtcc ttttgatttc cacatttgct
      481 cacaagtgcg ctgcaagcag aagcaaaggt cgaaatcgac cattagttcg cctgatttgg
      541 ggatgatgcg caagaggccc aagagggtcc ccgaatatga cctcggccag caacccatcg
      601 ctgcgcccgt tagacggcga aaacaaagga ttttcaccga attgaaccat gacgctcgag
      661 gcaaaattat aaccgcaagg cttataagac aaagatgaac acaacctgcg gtcgacctga
      721 cgtgctttaa tgtgcgcatg tcgaagagac cgggcgcggc aaatgtccgg ggccgttcgg
      781 cggacagatt ccgcgtcagg tacgctccag gttggcgttc taaatgtgta tcttgaattt
      841 accttgatgc aacgggagtg tttcatgtcc cagcaacatg aaattgaaga tgatctcgac
      901 gccgcgtatg tctcccgcgt ccggccccat atcgagcgtc tcgctcaggc tgaggaagac
      961 gtcgttcttg atttttccaa ggtggagttt gtggacagct ctggcctcgg cgcgctcgtt
     1021 tttcttcaca aacggctcgt atcaaaaggg cataagctgc gggtcgtcgg cttgcgaggg
     1081 caacccttgc agctctttac caatctacat cttgttccct tgttctgcga ctgaacgaaa
     1141 aagtcatccg gggacttgat taccgggatt ttcccgccga gtggcttcta cagggttttg
     1201 gcgctgtttt tgccgcgacg gtggcaagtg cgggaaccat gcggctcgga tcgaagggcc
     1261 ggttataacg atactcataa gcggcaagat atcgcgatgc gtgttgcgga cagatggcgc
     1321 gagtgatagc gcccttgatg ttgccgaggc agatattcgc ccctttgaac cttggatcga
     1381 caatgcgccg cgaggctcgg cctcgcgtga ttgttatgtg ttgtcatgcc agccttggca
     1441 aggcttgtga agcaagtgag gccatcgcta atcacctcca tccaggctcc aaggtttctc
     1501 gcgcatagct ttcgagcgcc tctttggtga agcccggaac caggcggagc ttggtgaggc
     1561 cggcttgccg ccacgggtct cgacagcggc gagaaaaggg gtcttaccct cggcgccgcg
     1621 tccgctcatc ccctttcttt cggcgccaag catcgtcgat ttgcgccgag cctttcaact
     1681 tgtaggtcgt tgcgttcacc catggctgtc atgagttttt gctttacaat ccaagccgta
     1741 tcgtacgtcg cgccgacctg gcgcgacagt tcaagggcgg aaatatcgtt cttactttgc
     1801 gtaacgagat aaatcgctag gaaccaggtg gtgagcggca gctttcgcct tgaccgaggt
     1861 ctgacgcctg cacagcgatc attggaaggc tcgcctcgca gcgtaaaagc tatatttgcg
     1921 tcccaggcaa gccgggcaga caaagccatc aggcccgcag ccggaccaac tcctcatgca
     1981 ctgtcttccc tgccgtaaag gtcttgaaag gccgcccggc tcatgtcctt ctgaaactgc
     2041 accggatttc gccccatcgg cttcctctat cactacgccc tattgttttc acaaaagaaa
     2101 aaaacaggaa catctacata atcaagtccg gggaagtcag attctggttg cctgcgagat
     2161 tttgtctaat gccgtgtgag aggagggcac gacccttcga cgctacattc catgaaacgc
     2221 gaaaaatcgt ccccgggatg ctgcctgaat gccatggctc ggcccacctt ggaagaaaca
     2281 gctccccagg gtattgacca attaacccca tgaacctatg gtcacctcga acttgcacgc
     2341 agtcacgttg cgtaagcagc cgatttttca aagagcccat agctcagccg gtagagcatc
     2401 tgacttttaa tcagagggtc gatggttcga atccatctgg gctcaccaat aaaatcaagg
     2461 ggttagccac gcgccgatgt tcgctcggtg ttcatatacg cactgtggac gcacctaaaa
     2521 caaaatatca gagcgcgatc ggcgaattga gtcgcgccct cacccagcga a
//
LOCUS       DATMGX010000373         2200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_63759, whole genome shotgun sequence.
ACCESSION   DATMGX010000373 DATMGX010000000
VERSION     DATMGX010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63759"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..686
                     /locus_tag="VNR65_06030"
     CDS             246..686
                     /locus_tag="VNR65_06030"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWJ18268.1"
                     /translation="MSTRAQVSSVAMNLLGLSFVGAAYAHHSFAAYDMTRSLTVQATV
                     KEFRWSAPHSSLVLMVTLPNGQARELNLVTGPPDIFARQGIAPKSLRSGDKLEATYHP
                     NINGSAGGSLAGFTLPDGRTFKDQESFGAAPPSSPSPSPSSSPR"
     gene            631..1416
                     /locus_tag="VNR65_06035"
     CDS             631..1416
                     /locus_tag="VNR65_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18269.1"
                     /translation="MALRRRPRHRLRHRHHHANTDVLAEMKGVCMFFAWLVGMSLSVL
                     CCLFCTPTAFAETILDGTWKIERPRAILEPINGTVPFTPKARKHYQANKRAHDSGHYD
                     YDLVQTLCATPGLPRLMLTPDRFHIWQRPELITFQFEWNRLFYQVDMTDRAIEPLLVG
                     VNTGESQGQWQGDTLVIKTNNFTDQTLIDSLVPHSDEMKLTQRFRLLDSNTLEDRITV
                     EDPQTFTRPWEAMVRYSRQPDAPFPENMCIDRRNAGKLPLPPK"
     gene            1752..>2200
                     /locus_tag="VNR65_06040"
     CDS             1752..>2200
                     /locus_tag="VNR65_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18270.1"
                     /translation="MLPLTRKWPSPSPDPRNFEGVWRQYNILPLERSTDMYGARTPLN
                     DAGRSLLAKRVAAAHAGTPYVNASAVCLPPGQPLQLEINLPFVVHQNKDWMQFLFYQY
                     HGAWTVVFDPAKQPDGNVRPYMGRSVAHWETDTLVVETIGFKQALWL"
BASE COUNT          446 a          690 c          611 g          453 t
ORIGIN      
        1 cgagtccccg cttgcagcca ccggcgccca ccatatctca ttccgatatg gcccaaaccg
       61 aactcagcat tggttccgac gaatcctcac tgccatagta tctcagcacc gttgggcgaa
      121 acctcgcctt gcaaacgagg cggcgggtgg acggcgccaa ccaggcgttg cctctgaaga
      181 aggggtcgat tgttctaatt cgagacgagt catggcggga actgaggtgg gtgaggagct
      241 gtccgttgag cacaagagcg caagtgagtt ctgtggcgat gaatttgctc ggattgagtt
      301 tcgtcggagc cgcctatgcc caccattcat tcgccgctta cgacatgacc cgtagcctga
      361 cggtgcaggc aaccgtcaaa gagttccgat ggagcgcgcc ccactcgagt ctcgtactca
      421 tggtcacatt acccaacggc caagcgcggg aactgaatct ggttaccggg cccccggata
      481 tctttgcccg ccaaggcatc gcgcccaaga gcttgcgctc cggggacaag ctggaagcga
      541 cttatcaccc gaacatcaac ggctccgccg gcggctcgct ggccggcttc accttgccgg
      601 acggtcggac attcaaggat caagaatcct ttggcgctgc gccgccgtcc tcgccatcgc
      661 cttcgccatc gtcatcacca cgctaacacg gatgtgctcg cagaaatgaa aggtgtgtgt
      721 atgttttttg cttggctcgt tggtatgagc ctcagcgttc tgtgttgttt gttctgcacc
      781 cctacggcgt ttgccgaaac catcctggat ggcacctgga agatcgagcg gccacgtgcg
      841 attctcgagc cgataaacgg cacggtacct ttcacgccca aggcgcgaaa gcattaccag
      901 gccaacaagc gcgcgcacga ctcaggtcat tacgactacg accttgtgca aacactttgc
      961 gcgacgccag gccttccacg cctgatgctg acgccggatc gctttcatat ctggcagcgg
     1021 cccgaattga ttactttcca attcgaatgg aatcgtctct tctaccaggt cgatatgacc
     1081 gatcgcgcaa tcgaaccgtt gctggtcggc gtcaacaccg gcgagtccca agggcagtgg
     1141 caaggtgata cgctggtcat caaaacgaac aatttcaccg atcaaacatt gatcgactcg
     1201 ctcgtacccc atagcgatga aatgaagttg acgcagcgat ttaggctgct tgattccaac
     1261 acccttgaag atcgcatcac ggtagaggac ccgcagactt tcacgcgccc gtgggaagcg
     1321 atggtcagat atagccgtca gccagacgcg ccatttccgg aaaatatgtg cattgaccgg
     1381 cgcaacgccg gcaagctgcc gttgccgcca aaatgaggac gccggtgttt gctcacatta
     1441 gatcttttgc ctgctcaagc gcatttgtgc tgcatcgagc cgtccttacg gccctaggcg
     1501 tagcctgctg gatcgcgact gcgcagctta cccacgctca gagcccttcc ccaccaagcg
     1561 gcgcggttcc gccgggtgtt cctgcccccc cgcccggagc ggcgccgcct ggccttttgg
     1621 gccctccgcc tggcgttgtg ggccctccgc ccggcgcttc tggagcaccg cccagcggtt
     1681 cgcccccccc gcccggcgga tcgccgtctg atgcggccat ctcggcattc cgggacaaat
     1741 tggcggcaga cttgctgccc ctgacgagga agtggccctc accatcgccc gatccgagga
     1801 atttcgaggg tgtctggcga caatacaata ttcttccact tgagcgctcc acggatatgt
     1861 acggcgcaag gacaccgctc aacgacgcgg ggcgatcgct gctcgcaaag cgcgtggccg
     1921 ctgcacatgc aggaacaccc tatgtgaatg cctcggcggt gtgccttcca cctggccaac
     1981 cgctccagct cgaaatcaat ttacccttcg tagtccatca gaataaggat tggatgcaat
     2041 tcctgttcta tcagtaccac ggcgcctgga cggtggtatt tgatccagcg aagcaaccgg
     2101 acggcaacgt tcgtccgtac atggggcgct cagtcgcgca ttgggaaacc gacacattgg
     2161 tcgtggagac aatcggcttc aagcaggcgc tttggctgga
//
LOCUS       DATMGX010000374         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_132143, whole genome shotgun sequence.
ACCESSION   DATMGX010000374 DATMGX010000000
VERSION     DATMGX010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132143"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /gene="motA"
                     /locus_tag="VNR65_06045"
     CDS             complement(<1..730)
                     /gene="motA"
                     /locus_tag="VNR65_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HWJ18271.1"
                     /translation="MFAIIGIVVVFGCIVAGYLMEHGNLRVLIQPAELIIIGGAALGT
                     LLVANPLHIIKKIAAGIGGVFGSSKFGKQTYIDTLKMMYDLLNKARKDGLMALEADVE
                     EPEKSQVFTKNPAFLKNHHVRDFVCDSLRMAITGIDAFDLDQMLDLDLEVHHQDSVAP
                     TAALSTVADSLPGLGIVAAVLGVVICMGALGGPPEEIGHKVAAALVGTFLGILLCYGL
                     VGPIAANMTKAAEEEHAFLYVMRVL"
     gene            874..1092
                     /locus_tag="VNR65_06050"
     CDS             874..1092
                     /locus_tag="VNR65_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18272.1"
                     /translation="MDGGFTDRDWEGATFSPPFDAVAGEHATDALPNGGQGFLAAAIE
                     DDEELVVTPAPDEVCGPERAFEGTRDHA"
     gene            1131..1526
                     /locus_tag="VNR65_06055"
     CDS             1131..1526
                     /locus_tag="VNR65_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18273.1"
                     /translation="MGQAFESHAKHCERYLDFDKGRKRAAQVGQHQGVAGDVGDFVDA
                     AGECEAAVAMNRRGGLLQFFKRFDGADNGAMLVVNGHGADADRDFVSGFVVQESDGLS
                     RVRSFDSAGDGTIFFAEFATGLIAVQQGL"
     gene            1536..1682
                     /locus_tag="VNR65_06060"
     CDS             1536..1682
                     /locus_tag="VNR65_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18274.1"
                     /translation="MADDVVAQVAGDAFGAVAPDDNFLLHVDNAEPGGEALENAAADV
                     GIMK"
     gene            complement(2254..>2655)
                     /locus_tag="VNR65_06065"
     CDS             complement(2254..>2655)
                     /locus_tag="VNR65_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWJ18275.1"
                     /translation="TNSVRVELQPDSSPHPVPDAPANDATDTAANLTTEAAEHVQISS
                     DTAEIVSRPVKPSYPLLARQMKVQGSVILQALISRNGSIQDLRVLSGPPILASAAQEA
                     VKQWHFKPHLQGTEPVETQTRITVNFTISTN"
BASE COUNT          522 a          679 c          909 g          545 t
ORIGIN      
        1 tgagaacgcg catgacgtag aggaaggcgt gttcttcttc ggcggccttg gtcatgttgg
       61 cggcgatggg gccgaccaat ccgtaacaga gcagaatgcc gaggaaggtt ccgaccagag
      121 cggcggcgac cttgtgtccg atttcttcgg ggggaccacc gagagcgccc atgcagatga
      181 cgacgccgag cacggcggcg acgatgccga gaccgggcag ggaatcggcc acggtgctga
      241 gggcggcggt cggtgcgacg gaatcctggt ggtgaacttc gaggtcgagg tcgagcatct
      301 ggtcgagatc gaaggcgtcg atgccggtga tggccatgcg gagcgaatcg cagacaaaat
      361 cgcgaacgtg atggttcttg aggaaagctg gattcttggt gaagacctga cttttttccg
      421 gttcttccac gtcggcttcc agggccatga gcccatcttt gcgggccttg ttgagcaggt
      481 cgtacatcat tttcaaggtg tcgatataag tctgcttgcc gaacttcgaa ctaccgaaca
      541 cgccgccgat tccggctgcg atctttttga tgatgtggag cggattggcg accagaagcg
      601 ttcccagggc ggcgccgccg atgatgatca attcggccgg ttgaatgagg acgcggagat
      661 tgccgtgctc catgaggtaa cctgccacaa tgcaaccgaa aacgacgacg atgccgataa
      721 tcgcaaacat tgctaactcc cggatgatcc tctcgaagga gatccgttgc aaaacgaacc
      781 gttcgaagcg gagggagcat tggttcgcca ggcggaagct gcctccagtg atttctgaac
      841 acgctgcatc agggcctcaa cagtgtcgcc gggttggacg gaggcttcac cgaccgcgac
      901 tgggagggag cgacgttctc cccaccattc gatgccgtcg ccggagagca tgcgacggac
      961 gcgctcccga acggagggca gggtttcctc gcggcagcca ttgaggatga cgaggaactg
     1021 gtcgtcactc cagcgcccga tgaagtctgt ggtccagagc gtgctttcga gggaacgcgc
     1081 gaccacgcgt aataaggagg aagccgcctc ggtccgaggc tggcgcggaa gtggggcaag
     1141 ccttcgagtc gcacgctaag cactgcgaac ggtacctgga cttcgacaaa ggtcgcaagc
     1201 gtgcggcgca ggtgggacag catcaaggcg tggctggcga cgtcggtgac ttcgtcgatg
     1261 cagccgggga gtgtgaggct gccgtcgcga tgaatcggcg cggcggtttg ttgcagttct
     1321 tcaaacgttt cgacggcgcc gacaatggag ccatgctggt tgtgaacggg cacggcgcgg
     1381 acgcggaccg ggatttcgta tccggctttg tggtgcagga atccgatgga ctcagtaggg
     1441 tgcgaagttt tgatagcgcg ggcgacggga caatcttctt tgcagaattc gcaaccgggc
     1501 tgatcgcagt gcagcagggt ctctgagatg caggagtggc cgatgacgtc gtggcgcaag
     1561 tggccggtga cgcgttcggc gccgtcgctc cagatgacaa ttttcttctg catgtcgaca
     1621 acgcagagcc cggcggggag gctctcgaga atgctgcggc agatgtcgga atcatgaaat
     1681 agggacatgc gtggtcgcgg gaagatcctc gactgttggg gacatcggca aaatccacag
     1741 ggacttcaga gaacgcgatc agggtgtttc cggcgcgagc gtgggaatcg ccgatttttc
     1801 agagagatag gagggaggcg cggggaaagt ggatatggca cagaagtgcc ataggcaggg
     1861 gagcgagtgc cattctattt tcttcggtga gcagttctgg gggagggctg cccgggcgtc
     1921 ctggactcga aagagtttgg ggcgctcgtt attttttgag atcttttcgt ccacgcagtc
     1981 caggtgggtc ccttagcgta acgtcccatc cgtatctctt gttcaaggct tccagaggtt
     2041 gcggaagaac gcggccgatc ctggaacaat cgaccacagg ggctgaagcc cacactaaat
     2101 ccacatgcct tacgcggccc tggaagggcc gctcttccac ggtagtgcac gcgttcgtag
     2161 ttcttccaca acctcttcca gacgcattcg caggctgcgc tcgcaactgg ggcggagccg
     2221 cttcgccgat ttcgccgggg gctgcgagga aaatcagttg gtggaaatgg tgaagtttac
     2281 tgtaatcctc gtttgagttt cgacgggttc cgttccttgc aagtgcggct tgaaatgcca
     2341 ctgcttgacg gcttcctggg cggcgctggc caggatcggc gggccgctga gaacgcggag
     2401 atcctggatc gagccgttcc tgctgatcaa tgcctgtaga atcaccgagc cctgtacctt
     2461 catttggcgg gccagcagag gatagctggg cttcacgggc cgactgacga tttcagcggt
     2521 gtcagaggag atttgcacgt gctcggcggc ttcggtggtg aggttggctg ccgtgtcggt
     2581 tgcgtcgttg gctggggcgt cggggactgg atgaggagaa gagtcgggtt ggagttcgac
     2641 tcgcactgag ttggt
//
LOCUS       DATMGX010000375         2289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_231423, whole genome shotgun sequence.
ACCESSION   DATMGX010000375 DATMGX010000000
VERSION     DATMGX010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231423"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VNR65_06070"
     CDS             complement(<1..335)
                     /locus_tag="VNR65_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18276.1"
                     /translation="MSLNSISEHAPSVGRSNAKWIGWTVAALTVWGVVYSQLVPFADW
                     VVASTGVDPNSHLGEALSFFAYDTPKVLMLLTLVVFTMGVVRSFFSPEKTRALLAGKR
                     EGVGNVLAAS"
     gene            complement(332..625)
                     /locus_tag="VNR65_06075"
     CDS             complement(332..625)
                     /locus_tag="VNR65_06075"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWJ18277.1"
                     /translation="MNPIAIFSALAEPVRLEALRMVWDGREHCVCELMRELGASQSRM
                     SRHMAALKTAGLVVDRRDAQWVRYRRSPNLPREVVALVDAALALPANEERHAA"
     gene            complement(706..927)
                     /locus_tag="VNR65_06080"
     CDS             complement(706..927)
                     /locus_tag="VNR65_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267462181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HWJ18278.1"
                     /translation="MQLVSTITCPACGHRASETMPTDACLFFCVCKGCGIRLKPRKGD
                     CCVFCSYGDVPCPPIQEARAKGENTGCYN"
     gene            complement(979..2166)
                     /locus_tag="VNR65_06085"
     CDS             complement(979..2166)
                     /locus_tag="VNR65_06085"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWJ18279.1"
                     /translation="MANRDVVICSPVRTAIGTYGGTFKEMPATDLGAAVVRATLDRAK
                     LQPGQVGTIVMGNVIQAGNKMNPARQAAINGGVPVEVPAMTVNRVCGSGAQAVASAAQ
                     EIMLGFIDSAVAGGMENMDRAPYLMNSGRWGYRMGDAQIFDSMLMDGLNDAFSGQHSG
                     WHTEDLARSHQITREDQDRWAERSQKRFSAAQAAGKFTAEIAPVEVFSRRGPVKFDKD
                     EHNRPDTTMESLSKLKPAFRKDGTITAGNAPGLNTGAAAMVVADRAWAEKKGLQPMAR
                     LAAYGIGAVEPGMFGLGPVPAVKQALDRAGWKIGDVGRIEINEAFAAIVLAVIKDLGL
                     SPDVVNVEGGAIAHGHPIGASGAVLATRLLHSMQRDGIDKGVVTLCIGGGQGIALALE
                     RIN"
BASE COUNT          455 a          706 c          680 g          448 t
ORIGIN      
        1 gacgcagcca gcacgttgcc gacgccttcg cgcttcccgg caagaagcgc ccgggtcttc
       61 tcgggcgaga agaagctgcg cacgacgccc atcgtgaata cgaccagcgt gaggagcatc
      121 agaactttcg gcgtatcgta agcgaagaag ctgagcgctt cgccgagatg gctattggga
      181 tcgacgcctg tcgaggcgac gacccaatcc gcgaatggaa cgagttggct gtagacgacg
      241 ccccaaacgg tcagggccgc gaccgtccat ccgatccact ttgcgttcga acggccgacg
      301 ctcggcgcat gttcgctgat ggaattgaga ctcatgcggc atgcctttcc tcatttgcgg
      361 gtagcgccag cgcggcgtcg acaagcgcca caacctcacg aggaaggttc ggcgagcggc
      421 ggtagcggac ccattgcgcg tcgcggcggt cgaccactag gcctgcggtc ttgagggccg
      481 ccatgtggcg ggacatgcgg gactggctgg caccgagttc gcgcatcaat tcgcaaacac
      541 aatgctcacg cccatcccac accatccgga gggcttcgag ccggaccggt tctgcgaggg
      601 cggaaaaaat tgcgattgga ttcattagct taacctttaa atgcgcttat gcgcataacc
      661 gcatataagc aaccttctat cgattatgca agaccctcca gaaaatcagt tgtagcagcc
      721 ggtattctca cctttcgccc gcgcttcctg aattggcggg caagggacgt cgccatagga
      781 gcaaaatacg cagcaatcgc ctttcctggg cttgaggcga atcccgcagc ccttgcacac
      841 gcagaaaaac aggcaagcgt ctgtcggcat ggtctcagat gcgcgatgcc cgcaagcggg
      901 acacgtaatc gtcgagacga gttgcattcc acctcacaag gaagcgacat ctaggccgtc
      961 agaagcgggc gatggtcctt agtttatccg ctccaatgcc aacgcgattc cctgcccgcc
     1021 gccgatgcag agtgtgacga cacccttgtc gatcccgtcg cgctgcatgg agtgcaagag
     1081 acgcgtcgcc agcaccgcgc ccgacgcgcc gatcgggtga ccgtgggcga tcgcgccgcc
     1141 ttccacgttg accacatccg gcgacaaacc gaggtccttg atgaccgcga ggacgatagc
     1201 cgcaaaggct tcattgatct caattcggcc gacatcgccg atcttccagc cggcacggtc
     1261 gagggcctgc ttcactgctg gtacgggacc aaggccgaac atgccgggct cgacggcgcc
     1321 gataccgtag gccgcaaggc gggccatcgg ttgcaggcct ttcttctctg cccaagctct
     1381 gtcggcgacg accatggccg ctgctccggt attgaggccc ggcgcatttc cggctgtgat
     1441 tgttccgtcc ttgcgaaacg ccggcttcag ctttgacagg ctttccattg tcgtatcggg
     1501 tcgattgtgt tcatctttgt cgaatttcac agggcccctt cgagagaaca cctcgacagg
     1561 ggcgatttcc gcggtgaatt ttccggccgc ttgcgctgcc gaaaaccgtt tctgtgagcg
     1621 ctccgcccag cggtcctggt cttcccgggt aatctgatgg gaccgtgcga ggtcctcggt
     1681 atgccaacca gagtgttggc cggagaacgc atcgttgagc ccgtccatca gcatcgaatc
     1741 gaagatctgt gcatcgccca tgcggtaccc ccagcgcccg gagttcatga gatagggcgc
     1801 tcgatccatg ttctccatgc cgcccgcgac tgcgctatcg ataaagccga gcatgatttc
     1861 ctgcgcagcg ctggcgacgg cctgcgcgcc cgaaccgcaa acgcggttga ccgtcatggc
     1921 ggggacctct acgggcacac ccccatttat tgccgcctgt cgggccgggt tcatcttgtt
     1981 gccggcctgg atcacgttgc ccatgacgat ggtaccgact tggccgggct gaagttttgc
     2041 gcgatcgagg gtcgctcgga cgaccgcagc gccgaggtcg gtagcaggca tctccttaaa
     2101 ggtaccgccg taggtgccga tcgcggtacg tacgggtgaa caaatgacaa cgtctcgatt
     2161 ggccatgact tgatctcctc gtttgagcgc tgaaacagcg cgatgccgtg gcaacgaagc
     2221 aagccagact cgacgagatg cccacggcgg ctacgtcatg gagataaatc catgaggatt
     2281 tatgcaatt
//
LOCUS       DATMGX010000376         2532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_447174, whole genome shotgun sequence.
ACCESSION   DATMGX010000376 DATMGX010000000
VERSION     DATMGX010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447174"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..746)
                     /locus_tag="VNR65_06090"
     CDS             complement(48..746)
                     /locus_tag="VNR65_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ18280.1"
                     /translation="MWRLHCAFDLPAERFSFIELTDEKGGERLDRTPFAKGDIVIADR
                     GFMHPDPLAQVLEQGADVIVRTPWKGARWYGKNGKPFDLLAALAAAETAGVLDRPIWV
                     LPKKAAPLPLRLVALRKPKQVAEASRATARAAAKWKGRAILSGTLIAAEWVILVTSLD
                     AKAFPARKIGELYRARWRIEMAFKRLKSLIGLSAPPSEDPQIAKTWILAHLLMILLLE
                     PHTGAPEISPRLAA"
     gene            complement(686..1141)
                     /locus_tag="VNR65_06095"
     CDS             complement(686..1141)
                     /locus_tag="VNR65_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18281.1"
                     /translation="MTHESLLNDLWQSALARFGGAPAVEASAREKGAFLRARGVASAA
                     DLLRLILAYCLGGMGLRSTSAWAASVGLADLSNVALLKRLRKCGPWMEHLASLLLSDG
                     VQPAAGGRRIRLVDGTTVPKAGREAKKTMGCGAFTAPSIFRPNASVLSS"
     gene            1847..2299
                     /locus_tag="VNR65_06100"
     CDS             1847..2299
                     /locus_tag="VNR65_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18282.1"
                     /translation="MDEFASRIESKVDQRGRVAIPAPFRNVLTQEGADEIHCYPHLDY
                     DAIEAGGARLVEEIKGIVGRQPAGSQERESLEIVYFGECEKLKIDPDGRTVLPKRLRD
                     HAGITETAVFVGLGNKFQIWEPEAYNKFRERARQQALALRRELGAGNR"
     gene            2299..>2532
                     /gene="mraW"
                     /locus_tag="VNR65_06105"
     CDS             2299..>2532
                     /gene="mraW"
                     /locus_tag="VNR65_06105"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HWJ18283.1"
                     /translation="MKRRKRGGKRGVTDRGRPEEGLSSNGGPVRHIPVLLAQVLRVLA
                     PEAGERYIDGTFGAGGYARAILEAADCEVLGLDR"
BASE COUNT          529 a          721 c          780 g          502 t
ORIGIN      
        1 caacgccttg gcggccaggc atgtcagacg ccaaagggct ggagctgtta tgcggccaag
       61 cggggagaga tctcaggcgc gcctgtatgc ggctcaagca gaagaatcat cagaagatga
      121 gccaaaatcc aggttttggc gatttggggg tcttcgcttg gcggggctga gagtccaatc
      181 aagcttttca ggcgcttgaa cgccatttcg atgcgccagc gggcgcggta gagctctccg
      241 atttttcttg ccgggaaggc ctttgcgtcg agtgaggtga cgagaataac ccactcggcc
      301 gcgatgagcg tgccgcttaa gatggcgcgg cctttccact ttgcggcggc tcgcgctgtt
      361 gcccgcgacg cctccgccac ctgtttgggc ttgcgcaaag cgacaagcct gagggggaga
      421 ggcgcggcct ttttgggcag gacccagatg ggcctatcga gaacgcccgc tgtttccgcg
      481 gcggcaagag cggccaggag atcgaagggc ttgccgttct tgccgtacca gcgcgctcct
      541 ttccagggcg tgcgcacaat gacatcggca ccctgctcca aaacttgcgc taacggatcg
      601 ggatgcatga agccgcgatc agcgatgacg atatcccctt tggcgaaggg cgtgcgatcc
      661 agccgctcgc cgcccttctc atcggtcaac tcgataaaac tgaagcgttc ggccggaaga
      721 tcgaaggcgc agtgaaggcg ccacatccca ttgttttctt tgcctctctc ccggctttag
      781 ggaccgtcgt cccgtcgaca aggcggatac gccgtcctcc cgccgcaggc tgtacgccgt
      841 cgctgagcag taagctcgcg agatgttcca tccaggggcc gcatttgcgc aagcgcttga
      901 gcaaagcgac gttcgaaagg tccgcgagac ccacggaggc ggcccaggcg ctcgtcgacc
      961 tcagccccat gcctcccagg caataagcaa ggataagccg cagcaaatcg gcggcgctgg
     1021 ccacacccct cgccctcaaa aaagccccct tctcgcgcgc ggatgcttca acagctggcg
     1081 ctccgccaaa ccgggccagc gcggattgcc agagatcgtt caaaagcgat tcatgagtca
     1141 tgccatgcta gaatctgact cgattccgca tggcaagcct taagttagcg cctatgcggg
     1201 cttgacccgg ccatccagtc atgaattgaa ataccttgtg tttttctgga tggccgcctc
     1261 caagggcggc catgacgacg gaaatggcga aaaacatccg aggtaatcaa gaaagcgaat
     1321 aagtgctgaa gcgttgttcc tcggctggtc agctggaagg aagcttttcg gctaatttct
     1381 caggcttggt gttaacccga tgcgcggctg aacaggccgg agagttcctg agagggaagg
     1441 ggctctcgtt tgtccgccaa tgcccgaccc ccggttaaaa ctttactaac caaattatga
     1501 cagatcgtct gcgcctgttg acgcccataa atacccatgc tatcccatag atgcccgttc
     1561 ggaagggggt gattcggggg gagatataaa atgctttcgt cgtgcacggc cactgcacgc
     1621 caaggtatct ccctccggtt ccgtccgaca cggtctaagg tcattgattt ggctattcat
     1681 ttgtaacttt gcttcactga tttcccgtta atgcgcgaga acaagacatc gcgcgttcct
     1741 gtttccgtcc ctgggtaggc atgggtagag acgggaaggg gaagatccga agcaatcgat
     1801 gcgcggccgt ctcgcgcgcg ttcggcgggg gcgtggaatt tgagggatgg atgaattcgc
     1861 atcacgcatc gagagcaagg tcgatcaacg gggacgcgtc gcaatacccg cgccgttccg
     1921 caacgtcctt acccaggaag gtgctgacga aattcactgc tatccgcatc tcgactacga
     1981 tgcgatcgaa gctggcggcg cgcgtctggt cgaggagatt aaagggattg tggggcggca
     2041 gcccgcaggt tcgcaggagc gcgaatccct cgaaatcgtc tatttcggag agtgcgaaaa
     2101 actcaaaatc gacccggacg ggcgcaccgt gctgccaaag cgcttgcgcg atcatgccgg
     2161 tattacagag acagcggttt tcgtcggcct tggcaacaag tttcaaatct gggagcctga
     2221 ggcttacaac aaatttcgcg aacgcgcgcg ccaacaggca ctcgcgcttc gcagagagct
     2281 cggtgcgggg aaccgctgat gaagcggcgc aaacgagggg gcaaaagggg agtgacggac
     2341 cgtgggaggc cggaagaggg cctgtcttcc aatggcggac cggtccgtca catcccggtt
     2401 ttgttagcgc aagtgctgcg ggtgctcgca ccagaggccg gcgaacgata cattgacggc
     2461 acattcggtg cgggcggtta cgctcgcgcc attcttgagg cggctgattg cgaagtgctc
     2521 ggccttgacc gc
//
LOCUS       DATMGX010000377         3724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_422715, whole genome shotgun sequence.
ACCESSION   DATMGX010000377 DATMGX010000000
VERSION     DATMGX010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422715"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VNR65_06110"
     CDS             <1..586
                     /locus_tag="VNR65_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWJ18284.1"
                     /translation="PTALKRKRKKLMSRIHTIPSIEAAPASAKPLLESAKKKLGSVPN
                     LFLVIANSSAGLEGYFGLDAGLAKGALDAKTRERIALAVAEVNGCSYCLSAHTYLGRN
                     VAKLDDTEIAANRNGASSDLKADAALRFAVKVVRERGHVSDADVAAVRAAGFDDGQIV
                     EIVLHVALNTLTNYVNEVAKTEIDFPVITLRKAA"
     gene            677..1057
                     /locus_tag="VNR65_06115"
     CDS             677..1057
                     /locus_tag="VNR65_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18285.1"
                     /translation="MHLRFVTRTVHAYLDYPVAIALIGLPFILGLGEANPIAKWLSVA
                     TGVAAFVLTLLTEHETGVIRVLPYPFHVAVDRLVGLVFVAAPFALGFGGLDAIYYWGF
                     GATVLIVTFLLNAPHQHSLQLAAA"
     gene            1138..1482
                     /locus_tag="VNR65_06120"
     CDS             1138..1482
                     /locus_tag="VNR65_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HWJ18286.1"
                     /translation="MLLYRRIYEPAEPEDGFRILVDRLWPRGLKRENAAFDEWLKLIA
                     PSNELRTWYGHRPERWSEFEARYQGELTSAEAVRELGRLREIAASGTLTLLTATRAIE
                     TSHLAVLRELLG"
     gene            1742..3178
                     /locus_tag="VNR65_06125"
     CDS             1742..3178
                     /locus_tag="VNR65_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HWJ18287.1"
                     /translation="MKRFIEGADRGQWTLLPESLDDWIAEDNPVRAIDAFVDALDLAE
                     LGFKVQPAETGRPSFHPSVHLKLYIYGYLNRVQSSRRLEREAGRNLEVIWLLGRLVPD
                     DKVIADFRKDNGPAIRKVCAQFVELCRRLGLLTKASVAIDGSKFKAVNNRDKNFTSGK
                     VERRRKQLEESVARYLSQLDTADLQEPTEALAAKTAHLKEKIAKIGEEMKKLDAIEKQ
                     MLASPDKQISLTDPDSRSMATSGRGSGMVGYNVQTAVDTENHLIVAHEVTNVGHDRSQ
                     LANMAKQAKAALQADTLEAVADRGYFDGEEILACDEADITVTLPKPMTSGSKSEGRFG
                     KQDFVYISGEDVYRCPAGEHLKYYFTTVERGLTLRRYWTNACQSCAMKAQCTKGKQRR
                     ITRWEHEHVAEAVQQRLDENPEAMRVRRETAEHPFGTLKARMGATHFLMKTLPKVSAE
                     MALHVLAYNFTRVMNIIGVKPLMAAIAA"
     gene            3410..>3724
                     /locus_tag="VNR65_06130"
     CDS             3410..>3724
                     /locus_tag="VNR65_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18288.1"
                     /translation="MSNKALTRILWQFHLSWASSNVLRRQGLHFRRNAVHNGALSFRA
                     SKTMKQRVFSGVQPTGNLHLGNYLGAITKFVALQENYDCVYCVVDMHAITVKQDPADL
                     RRS"
BASE COUNT          811 a         1099 c         1084 g          730 t
ORIGIN      
        1 ccccacggca ctcaaacgaa aaaggaagaa actcatgagc cgcatccata ccataccctc
       61 aattgaagcc gcgcccgcat cggccaagcc tctccttgag agcgcgaaga aaaagctcgg
      121 cagcgttccc aacctgttcc tcgtcattgc caacagctca gccggcttgg aaggctattt
      181 cggcctcgat gccgggctgg cgaaaggcgc gctcgacgcc aaaacgcgag agcgcatcgc
      241 tctggcggtc gccgaggtga atggctgctc ttactgtctt tcggcccata cttacctcgg
      301 ccggaacgta gcaaagctcg acgataccga gatcgccgct aaccgcaatg gcgcctccag
      361 cgatctcaag gcggacgccg ctctacgctt tgccgtcaag gtcgtccggg aacgtggtca
      421 tgtgtcggat gccgatgtcg cggccgtaag ggctgcgggt ttcgacgatg gacagatcgt
      481 cgagatcgtc ctccacgtcg ctctcaatac actcaccaat tacgtcaatg aggtcgccaa
      541 gaccgagatc gacttccctg tgattacatt gcgcaaagcg gcgtaatcgc gggccggcgg
      601 gatggttcga cccacgcccg cacccccttc tggaggggaa tcctaaacat caaacttcaa
      661 acattggaga aagatcatgc atcttcgctt tgtcacaaga actgtccacg cctatctcga
      721 ctacccagtt gctatagccc tgatcggatt gccattcatt ttggggcttg gagaggctaa
      781 cccaatcgcg aaatggctat cggtcgcaac cggcgttgcc gctttcgtgc tcaccctcct
      841 gaccgaacat gagacgggcg ttattcgcgt ccttccttac ccattccacg ttgccgtgga
      901 ccgtctggtt ggcttggtgt tcgtcgcggc gcccttcgcg ctcggctttg gcgggctgga
      961 tgccatttat tactggggct tcggcgccac cgtgttgatt gtcaccttcc tcctcaacgc
     1021 gcctcaccag cacagccttc aacttgccgc cgcttgaggc cgcgggcttg gacaagtgca
     1081 gcggcggcac cgtcgccgct gcagtcaacg aaaacaaaca aattactgag attgagaatg
     1141 cttctatata gacggatcta cgaaccggct gagcccgaag atggtttccg gatcctcgtg
     1201 gatcggctgt ggccgcgcgg actgaagcgc gagaacgcag cttttgacga atggttaaaa
     1261 ctcatcgctc cgtcaaatga attgcggact tggtacggtc atcgtcccga gcgctggagt
     1321 gagttcgagg cacgctatca aggcgagctc acttccgcgg aagcggtccg tgaacttgga
     1381 cgtctccgcg aaatagctgc atctggaaca ctcacattac tcaccgccac gcgtgcgatc
     1441 gagaccagtc accttgccgt tttgcgcgag cttctcgggt agccgctgac ggaccactgc
     1501 ggcgtggtgg gtggatacga gcttcgagga tccgagacaa ttcttgttgt gtgaccgaaa
     1561 tggcagagat gggtcaggtg cgggattgtg ttggccttct cggagaagac cgcatcagga
     1621 tcccgaagcc gtcttggcgt gcatgggatc gcgagaaaac cggcacgacc gcaatcgacc
     1681 ctggctgcgt gaaaacggcg agtcgggtat gattctcctc gcgatttgca gcgggggctt
     1741 gatgaagcgc tttattgagg gggcggaccg ggggcagtgg actttactgc cggaaagcct
     1801 cgacgattgg atcgccgagg ataatcctgt ccgggccatc gatgcttttg tcgatgcgct
     1861 cgaccttgca gagcttggct tcaaggtgca gccggcagag acaggccggc cttcctttca
     1921 tccttctgtc cacttgaagc tttacatcta cggctatctg aaccgggtgc aatcgagccg
     1981 ccggcttgag cgcgaggcag gccgcaatct tgaggtcata tggctgcttg ggcggctcgt
     2041 tcccgacgac aaggtgatcg ccgatttccg caaagacaac ggtcccgcca tccgcaaggt
     2101 ttgcgcccag ttcgtggagc tgtgccgccg gctgggtctt ctgaccaaag ccagtgtcgc
     2161 catcgatggc agcaagttca aagcagtgaa caatcgcgac aagaacttca cgagcggcaa
     2221 ggttgaacgg cggcgcaagc agcttgagga gagcgtggcc cgctatctga gccaactcga
     2281 caccgccgat ctgcaggagc caacagaagc gctggctgcc aagaccgcac atctgaaaga
     2341 gaaaatcgcc aagatcggcg aggaaatgaa aaagctcgac gcgatcgaga agcagatgct
     2401 ggcctcgcca gataagcaaa tttcgctcac cgatcccgac agccgctcca tggcgaccag
     2461 cggccgcggt tctggcatgg tcggttacaa cgtgcagacc gcggtggata cggaaaacca
     2521 tctgattgtt gcgcacgagg tgacaaacgt cggccacgac cgctcccagc tcgcaaacat
     2581 ggcaaagcaa gcgaaggcgg cgctgcaagc ggacacactc gaagccgttg ctgatcgcgg
     2641 ttacttcgat ggagaggaaa tcctggcctg cgatgaggcc gacatcacgg taacgttgcc
     2701 gaagcccatg acgtcgggct caaaatcgga aggccggttc ggcaagcagg acttcgtcta
     2761 tatctcaggc gaggatgtct atcgttgccc cgctggagag catctcaagt attacttcac
     2821 gaccgttgag cggggactga ctctgcgccg ctactggacg aacgcatgtc aaagctgcgc
     2881 gatgaaggcg caatgcacca agggcaagca gcgccgcatc acgcgctggg agcacgagca
     2941 tgtcgcggaa gcggtgcagc agcggctcga cgaaaacccg gaggccatgc gtgtgcggcg
     3001 ggagaccgcc gagcatccct tcgggaccct gaaagctcgc atgggggcga cgcactttct
     3061 gatgaagacg ctgccaaagg tctccgccga aatggcgctg catgtgcttg cctacaattt
     3121 cacgcgggtg atgaatatca tcggcgtcaa gcccctaatg gcggcgatcg ccgcctgagt
     3181 ggggcgcagc cggcgctttt tgctcctcag gagacagtcg aacccctctg gcgaagcatc
     3241 aggcttgccc aaagcccttc cgagaaaaaa aacgctcgaa aacgaccatt ttggaaaagt
     3301 ccgcaccgcc aagagccgtt cgagactcct cctgagcgtt ttcacgcggc ctggacccaa
     3361 ggcttccttc aagaaatgaa ggagtagcgg agcctacttg gaagtcggag tgtccaacaa
     3421 agcgcttacc cgcatcctgt ggcagtttca tttaagttgg gcaagcagca acgtgctgcg
     3481 ccgccaaggc ttgcatttcc gccgcaacgc ggtccataac ggcgccttgt cattcagggc
     3541 atccaaaacc atgaaacagc gcgttttctc cggcgtccag ccgaccggca atctgcatct
     3601 cggcaactat ctcggcgcga tcacgaaatt cgtagcgctg caggagaatt acgattgcgt
     3661 ctattgcgtc gtcgacatgc acgcgatcac ggtcaagcaa gacccggccg atttgcggcg
     3721 ctcc
//
LOCUS       DATMGX010000378         3780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_450220, whole genome shotgun sequence.
ACCESSION   DATMGX010000378 DATMGX010000000
VERSION     DATMGX010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450220"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..886)
                     /locus_tag="VNR65_06135"
     CDS             complement(71..886)
                     /locus_tag="VNR65_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HWJ18289.1"
                     /translation="MWSQIFGTAAFEGNGCWRVALAAKADRIGGTTIHRATIVFDLDG
                     TIADTAADLVDAANAALIAEGFRPASADAIRRGVGYGARAMLDHALSSSGQSADAIQF
                     GRLAGRLVQHYEEHIANKTRLFPGFLETANQLRGRGARFALCTNKLDRLVTKLLSALG
                     IATLFDAKAGRDTFPFHKPDPRHITELISRAGGALPFAIMIGDSEADIAAARGAGIPV
                     VAVRFGYAAVPPEDLGADAILDGFEDLPDLIGAFLPVKGGHPRLRLSKRAHEP"
     gene            complement(992..1333)
                     /locus_tag="VNR65_06140"
     CDS             complement(992..1333)
                     /locus_tag="VNR65_06140"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ18290.1"
                     /translation="MIRPLIFATALAAEFALPATAGKVPDQPLSPQDAKGWVGKRVYS
                     NDGKELGKVTRLDSSAENKATQLYTDIGSTTCPAKHQVILPATRFSLENDRIVIDMTA
                     AQTSKMPKMNY"
     gene            1731..2525
                     /locus_tag="VNR65_06145"
     CDS             1731..2525
                     /locus_tag="VNR65_06145"
                     /inference="COORDINATES: protein motif:HMM:NF018458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1217 domain-containing protein"
                     /protein_id="HWJ18291.1"
                     /translation="MSTLATYLRISNNLPKWQKLTASEPNVKQATKYYQENIGKVKTP
                     SDLVNNFRLFSYVLNAYGLGDQVYAKTLFRKVLEQGTDSNKNLAYTLNDPRILALAKT
                     FNFKANGASTTSSTAVQTDVVNSYVEQTLETNQGQSNPAVQLALYFQRNAPNIKTTYN
                     ILADKNLLTVVQTALGISPLTSAMNVDRQATLISNKLKVKDFQDPKKLATFIQKFSAL
                     YDASNAGATTTQSTSVPTAFINVSSNRTSFGVNLLSSIQSLKLGGF"
     gene            2670..3545
                     /gene="motA"
                     /locus_tag="VNR65_06150"
     CDS             2670..3545
                     /gene="motA"
                     /locus_tag="VNR65_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HWJ18292.1"
                     /translation="MTIVLGLLVTLGCMLGGFVFMGGHIAVIWQPAEYVIICGAAIGT
                     FIVANPVKVIKDSGKGIVEALKNAEPKESDYLAILGLLYALMKELKSKPKNEVEVHIE
                     NPESSPIFMRFPEVLHNEELTHFICDYARLHIMGSARPDEIEALMDEEIQTIRYDKLK
                     SYNALTTISEAFPALGIVAAVLGVIKAMAAIDQPPEVLGELIGSALVGTFAGIFLSYA
                     VVSPIATKIKTVREKKIRLYLLVKQTILAFMNGAMASVAVEHGRKMISAYERPSIDQV
                     ESNLTGSPEARKEAA"
BASE COUNT          882 a         1054 c         1011 g          833 t
ORIGIN      
        1 tccgccgtta acctggattc gtgaccctgg catgtgaagc cgcctcacga acagagcaaa
       61 cggggccacc tcaaggttca tgcgcgcgct tgctcaagcg gagccgtgga tgccccccct
      121 taacgggcag aaaggcgcca atgaggtctg ggaggtcctc gaagccatca aggatcgcgt
      181 ccgccccgag gtcttccggc ggaactgcgg cataaccgaa tcggacggca acgacgggga
      241 tgcccgctcc tctcgcggcg gcgatgtcgg cttcgctgtc gccgatcatg atcgcgaagg
      301 gcaaggctcc ccctgctctt gatataagct ccgtgatgtg gcgcgggtcc ggtttgtgga
      361 aaggaaacgt atcgcggccc gctttcgcgt cgaaaagcgt ggcgatgcca agcgcggata
      421 agagcttcgt tacaagccgg tcgagcttat ttgtgcaaag tgcgaacctt gcgcctcgcc
      481 cgcggagctg atttgcagtt tcgaggaagc cggggaacag gcgcgtcttg tttgcgatgt
      541 gctcttcata atgctgcaca agccgcccgg caagccgccc aaattgaatc gcatctgcgc
      601 tctgcccgga cgagctgagc gcgtgatcga gcatcgccct cgcgccatat cccacgcccc
      661 gccggatcgc gtccgcactt gcgggccgga agccttcggc gatcagagcg gcgttcgccg
      721 catcgacgag atcggccgca gtgtcggcaa ttgtcccatc gaggtcgaaa acgatcgtgg
      781 cacggtgaat ggtcgtcccc ccaattctgt ccgccttggc tgccagcgcg acacgccagc
      841 agccattccc ttcaaaggcg gccgtaccga agatttgcga ccaaatagag ccgcgttttg
      901 gctaatccac gtcagatttg cccacagcga ttataagggg tagcgcgctg ccgggttcac
      961 tcagcagccc gcccgcatgc ctcttttcgc tttagtagtt catctttggc atctttgaag
     1021 tctgtgccgc cgtcatgtcg ataacgatgc ggtcattttc gaggctaaag cgggtggccg
     1081 gcaggatgac ttgatgctta gcaggacacg tggtgctgcc gatatcggtg taaagctgtg
     1141 ttgccttgtt ttcagcgctg ctgtccagtc tcgtcacctt tccgagctct ttgccatcgt
     1201 tgctataaac ccgctttcca acccaacctt tggcatcctg cggcgataga ggctggtcgg
     1261 gaactttgcc agcagtagcg ggcaatgcga attcggcggc aagcgcggtc gcgaatatca
     1321 gcggacgtat catcactttg tctcctcctt gaaaagcgat acctaagccc ccccaacagg
     1381 ctgcgggctt cccttctcgc ggaatcgtga atcgccggca gcctcacagg caatggcaac
     1441 gcgagcgcgc ggccatgggc gcgcgctcaa tgattccgcg cattaatctt ttgctaacag
     1501 ccacgaacta ccttaggttg agcactgtct tttttctcgg aggaaaccga tgactgtatc
     1561 gccggtcagg gcaaatcatc tgtaccaatc ccaaaatgcg ccggctccaa cgcattccga
     1621 tgaaaagcgc gtcacgaaaa gcgccctcag cacaagctca agcggtgtgc aagcggcgct
     1681 tttgagcctc caacctggcg gcgtctcctc tagctgactt tggtgtcacc atgtcaactc
     1741 ttgccacata tctgcggatt tccaataatc ttcccaagtg gcagaaactg acggcctccg
     1801 agccgaacgt taagcaagca acaaagtatt atcaggagaa tattggcaag gtaaaaacac
     1861 catccgacct ggtcaacaat ttcaggctct tttcctatgt tcttaatgcc tacgggcttg
     1921 gggaccaagt ttatgccaag accttgttta gaaaagtcct ggagcaagga accgatagca
     1981 acaagaatct agcctacacg ctgaatgatc ccaggatatt ggctctggca aaaacattta
     2041 atttcaaagc gaacggggcc tcgacgacct cgtccacagc agttcaaacc gatgtcgtaa
     2101 acagttacgt tgagcaaacg ctggagacga atcagggtca gtccaacccg gccgttcaac
     2161 tcgcccttta ctttcagcgg aacgctccga atatcaagac aacctacaac atcctcgcgg
     2221 acaaaaatct cctgaccgtc gtgcagacgg ctctaggcat atcgccgtta acctccgcaa
     2281 tgaatgtcga caggcaagcg acgctgattt cgaacaaact gaaggtcaaa gatttccagg
     2341 acccgaaaaa gctggcgacc ttcattcaga agttttcggc gctttacgat gcgagtaatg
     2401 caggcgcaac aactacacaa tcgacatccg tgccgaccgc gtttattaac gtctcaagca
     2461 atagaaccag ttttggtgtc aacctccttt cgagcattca gagcctcaag ttaggaggtt
     2521 tttgacggct ctgcccaggc agatggccga cgcgcaccgg agtgaatgac gtcgacgcga
     2581 caaacttaga cgtattcttg aagcttccgc aatcagcttc atgcaagttg ggcaggttaa
     2641 cgcactcaac ttgaacaagg aggctggttg tgaccattgt tctgggattg ctggtgaccc
     2701 taggctgcat gctgggcggt ttcgtcttca tgggagggca tattgcggtc atctggcagc
     2761 ctgccgaata cgtgatcata tgcggagccg cgattggcac attcatcgtg gcaaatccgg
     2821 tgaaggtcat caaagactca ggcaaaggca tcgtcgaagc cttgaagaac gctgagccga
     2881 aggaaagtga ttatttggca atcctcggcc ttctctatgc actgatgaag gagctgaaat
     2941 ccaagccgaa gaacgaggtc gaagtccaca tagaaaaccc ggagagttcc ccgatcttca
     3001 tgaggttccc tgaagtcttg cacaacgagg aactgaccca cttcatttgc gactatgcgc
     3061 gcctgcatat catgggaagc gctaggcccg atgagatcga ggccctgatg gatgaagaaa
     3121 ttcaaaccat tcgctatgac aaactcaagt cctataacgc cctcactacc atttcagagg
     3181 catttcctgc gctcggcatc gtagcagccg ttctgggggt catcaaggca atggcggcga
     3241 tcgaccaacc gcccgaagtg ctgggagaac tgatcggctc ggccttggtt gggacctttg
     3301 ctggcatatt cctctcctat gcagttgttt cgccgattgc aacgaagatc aagaccgtaa
     3361 gagagaagaa gatccgactc tatcttcttg ttaagcagac gatccttgcg tttatgaatg
     3421 gcgccatggc gtctgtcgcg gttgaacatg gccgtaaaat gatttctgcg tatgaaaggc
     3481 caagtattga tcaggtggag agtaacttga ccggatctcc tgaggccagg aaggaagctg
     3541 cctagggcga atctcctcag tcggtgagtg cttgaaatca ctctttctga aaccgtgaca
     3601 tgcaactgga taatgaaccc ataggggccg ctacagtgaa cgacaaactc gtaccatctg
     3661 atagtcaagc cacgggcaat atggatcgag tgttacggat ccccgtcacc ctcaaagtcc
     3721 tgctcggtac ggcgtcgatg ccgatttttc aactgacaaa acttgggcgt ggcgctgtta
//
LOCUS       DATMGX010000379         2238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_277201, whole genome shotgun sequence.
ACCESSION   DATMGX010000379 DATMGX010000000
VERSION     DATMGX010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277201"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VNR65_06155"
     CDS             complement(<1..469)
                     /locus_tag="VNR65_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18293.1"
                     /translation="MQNVSILAAGPAERVRKLRRLRAKKLRRLGDGDARFRILTKLSA
                     IGVLIVLSAIFVSLMIGAALAFSTFGFSFFYTQGWNPVTGKFGALSPIVGTLVTSFIA
                     MLIAIPVGLGSAIFLTELCPPLLRRPIGIAIELLAGIPSIIYGIWGLFIFAPFL"
     gene            complement(599..1651)
                     /gene="pstS"
                     /locus_tag="VNR65_06160"
     CDS             complement(599..1651)
                     /gene="pstS"
                     /locus_tag="VNR65_06160"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWJ18294.1"
                     /translation="MRVSIKAFITGLAISMAAASAAVLAADISGAGATFPYPIYAKWA
                     DSYKKETGNELNYQSIGSGGGIKQITARTVTFGASDVPLTAKELAGAGDLIQWPMVMG
                     GIVPVVHIEGIKAGDITLDGPTLAKIFLGEIKTWDDAALKKLNPNVKLPSDAIAVVHR
                     SDGSGTTFNFTNYLGKISPEWASKVGSNTSVEWPVGIGAKGNEGVANNVQQAQNSIGY
                     VETAYAKQSKLTTTKMVNKDGKTVEADGASVKAAAANADWAKSDGFYLLITDQAGAGV
                     WPISASTFILMPKKVKDAASSEEALKFFAWAYANGDKAAEELDYVPMPENVKKLVIST
                     WAKITGPDSKRVLAAN"
BASE COUNT          533 a          609 c          599 g          497 t
ORIGIN      
        1 gtaaaaacgg ggcgaatatg aacaagcccc aaatcccata aatgatactg gggatgccag
       61 ccaggagttc gatggcgatg ccaatcggcc gtctcaagag aggggggcaa agctccgtca
      121 gaaaaatcgc tgagcccagt ccaacaggaa tggcgatcag cattgcgata aaagacgtta
      181 cgagcgttcc cacgatgggc gaaagcgcgc cgaacttgcc ggtgacggga ttccagccct
      241 gcgtgtagaa gaagctgaaa ccgaatgtgc tgaaggcaag cgcggcgccg atcataagcg
      301 acacaaaaat ggcgctcagc acaatcagga cgccaatcgc gctgagtttc gtcaagatgc
      361 ggaaacgcgc atcgccgtct ccgagccggc gcaacttttt agcgcgtagc cggcgcaact
      421 ttctaacgcg ctcagccggt ccggccgcca aaatggagac attttgcatg tgtcagcgcc
      481 cagctttcac aacgaggagc gggcttcacc tttcacaacg agaagagacg gacaccaagg
      541 cggtttcggg tgagggcgag ccgtagacac tcctcacccc ggcacaatca cgcttaagct
      601 agttcgcagc gagaacgcgc ttgctgtcag ggcccgtaat cttggcccat gtcgaaatca
      661 cgagcttctt gacgttttcc ggcatcggca cgtaatcgag ttcttccgca gctttatcgc
      721 cgttggcgta agcccacgcg aagaacttca gcgcttcttc cgacgacgcc gcatccttga
      781 ccttctttgg catcaaaatg aatgtcgatg cggagatcgg ccaaacgccc gcgccagcct
      841 ggtcggttat gagcagataa aagccgtcgc tctttgccca atcggcgttt gcggcggcgg
      901 ctttgacgga cgctccgtct gcctcaaccg tcttgccgtc cttattcacc atcttggtcg
      961 tggtgagctt gctctgcttg gcataggcgg tttcgacata gccgattgag ttttgcgcct
     1021 gctgcacatt gtttgcgacg ccttcgttac ctttggcacc gatgccgact ggccactcga
     1081 cagaggtgtt cgatccaacc ttggaggccc attccgggct gatcttgccg agataatttg
     1141 tgaagttgaa tgtggtgccc gatccatccg agcgatgcac gaccgcgata gcgtcagacg
     1201 gcagtttgac gttcggattg agcttcttga gcgccgcatc gtcccaagtc ttgatttcgc
     1261 ccaggaagat tttcgccagt gtcgggccat cgagggtgat atcgcccgcc ttgatgcctt
     1321 cgatatgcac cactggcaca atgccgccca tgaccatcgg ccactggatg agatcgccgg
     1381 caccggccag ctctttagca gtcaggggaa cgtcagaggc gccgaaagtt actgtccgcg
     1441 ctgtgatttg cttaattccg ccgcctgagc cgatcgactg atagttcagt tcgttgccgg
     1501 tttccttttt gtaactgtcc gcccacttcg cataaatcgg atagggaaag gtagcgcctg
     1561 ctcccgaaat gtccgccgca agaacagccg ctgatgcagc ggccatagag atggccaatc
     1621 cggtaataaa tgccttgatc gatactctca cgctttagct ccattgttaa ggcgagggat
     1681 accccagcca tcaaatgctt gcgcttaagc gtagaattat actcgtgaag tattacacta
     1741 ttaagacaac tcacgttatt tcgtaatata aaatattgca aaaaataccg aaagcttgac
     1801 cattgtgtaa ttcacatagt tgatcaataa acatcaacag aaattctatg atatgtgtat
     1861 cgcgttatta cgaaacacat agtgctaggt tagcataatc gttcattgga gattagtttg
     1921 aagcgccacc cgctgcagtc gtccgcagaa gcttggatcg gcgctgtgta ccgagatgta
     1981 tcgcaagggc aacaactaca cgggatgccg gacgacgaaa gagattgcga gcatgctagc
     2041 ttcgcaaaca tgtgcgatgg cgttagtgat cgggttgtgc cgtgaataat cggcttgccc
     2101 agtcgcatcg ctacagcaac ctaaccttac gctaggaagg acgcagacca tagactcgga
     2161 aactactcaa ggcagcgccg tgatgtcatg gccgggcttg acccggccat ccagcgtcaa
     2221 ggtccagaag atggaatt
//
LOCUS       DATMGX010000380         3901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_395148, whole genome shotgun sequence.
ACCESSION   DATMGX010000380 DATMGX010000000
VERSION     DATMGX010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395148"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VNR65_06165"
     CDS             complement(<1..622)
                     /locus_tag="VNR65_06165"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HWJ18295.1"
                     /translation="MRNTAALRNPNVRARIVAGAAMAGWYYGRSGSGWWQHSDGGYGW
                     VGPLFWPFAYLDIYDYAIWGNGFGAPFWGYGYDDIYAGMFAPYGYDDLTGYLPPRASA
                     APGKPSGAPDRLAQLCGEDSHDIAGLPIDLIQQAIEPTETQRAALDDLANVSVTAAQT
                     IKAACPTGVSLTAPSRLGFMQQRIEAMIAAVATVQPSLDRFYNVLND"
     gene            complement(673..963)
                     /locus_tag="VNR65_06170"
     CDS             complement(673..963)
                     /locus_tag="VNR65_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18296.1"
                     /translation="MTVPCQNSASGLPSLFSRPRCQLLGLPLRKVVVAVTAVVAVAAV
                     AVTVVVVTVVVDTAVVMVAGILAAALTVVVATWEGDRRTPVRLGDRIPAATT"
     gene            complement(1807..1977)
                     /locus_tag="VNR65_06175"
     CDS             complement(1807..1977)
                     /locus_tag="VNR65_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18297.1"
                     /translation="MVRLAGMRYGGRTVATALRMGRSGLSYRRHFTIRQPTLATQKAA
                     GICALRLPGFEN"
     gene            complement(2461..3015)
                     /locus_tag="VNR65_06180"
     CDS             complement(2461..3015)
                     /locus_tag="VNR65_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18298.1"
                     /translation="MRKSHFGAAPKRTLNRPFTISVILSILTVFGKVALQMNATSASE
                     DNLLSFTRSLPGDEGRTVLNLVFHAAELFSGMEEQVRAAEARAQSVCKSATERAQIAE
                     KRLDVAERARREVINEADRKLQDASKALMNAETRIAAAEDKVTALEFRAQSAEALLRE
                     AKRELLLVEEAIRERLLSEKPLTV"
     gene            complement(3137..3307)
                     /locus_tag="VNR65_06185"
                     /pseudo
     CDS             complement(3137..3307)
                     /locus_tag="VNR65_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          823 a         1190 c         1063 g          825 t
ORIGIN      
        1 catcgttcag gacgttatag aatctgtcca gcgatggttg caccgttgcc accgccgcga
       61 tcattgcctc aatgcgttgt tgcataaacc caagccggct aggcgccgtc agcgatactc
      121 ccgtcgggca ggccgctttg atggtctgcg cggccgtgac cgatacgttg gcgagatcgt
      181 cgagcgcggc acgttgcgtc tcggtcggct caatagcctg ctgaatcaga tcaatcggca
      241 ggccggcaat atcgtggctg tcttcaccgc acaattgggc caaccgatcg ggcgccccac
      301 tgggtttgcc aggtgccgcc gaggcgcgtg gaggcaaata gccagtaagg tcatcgtagc
      361 cgtaaggtgc aaacatgccg gcgtagatat catcgtagcc ataaccccag aacggagcgc
      421 caaatccgtt gccccagatc gcatagtcat agatatcaag ataggcgaac ggccagaata
      481 acggcccgac ccatccatat ccgccgtcac tgtgttgcca ccatccgctt ccggaccgac
      541 cgtaatacca accggccatc gccgcacctg cgacaattcg agcgcgtacg ttggggtttc
      601 gcagcgcggc cgtattgcgc aatccgcgcg caagtgcatg agagttcaac gcgttgcgtg
      661 ccgcgccgga cctcatgttg ttgcggctgg aatccggtcg cccaaacgaa cgggagttcg
      721 ccgatctccc tcccacgtgg cgaccaccac cgtgagcgcg gccgccaaaa tgcccgccac
      781 catgaccacc gccgtgtcca ccaccaccgt gaccaccacc accgtgaccg ccaccgccgc
      841 caccgccacc accgccgtga ccgccaccac cacctttcgc agcggcagac ccagcagctg
      901 acagcgcggt cgcgagaaca atgacggcaa tcctgatgct gagttttgac atggcaccgt
      961 catgctccgg tgcgccacta acctgtggca cgttcgagca acgcgggctt gatcagttgg
     1021 ttccgtcgcg gaacctgaat gggacggctc gtagttggag gccactggcg agtccgagag
     1081 cgcacggccg tctcatagtg gttcaatgac tgcgccgttg aatgctcgca cggcccagac
     1141 ttgcacccta ttcgtcgaga agggtatgca ccgcgctgac gagatgatcg catcgccacc
     1201 ccaggcggca tcgccggggc cgtcccgaac catcaacatg cgcgatgatg atccttggct
     1261 tcgttggcct tggcttcatg gcgcatcggc ggaaatcaaa gccagcattg atggctgcgt
     1321 gcagctatcg tcagatcgaa taaagagacc gccttcgggc ggtcttttgt tcgtgcgttt
     1381 attagtctga tcgaacccga gcaagcttcc gtcgctcggc cgcccacgcg atccccgctc
     1441 cgatcacgat cacagcgagc gttacgctca ggctgccggg cgcgacgcaa ttgatgcagc
     1501 tggttacaag cgtcggaagt tcgttcattt ggcactcttt cagaagctat gcccagttta
     1561 aatggaagta ttccgacgaa gtcaatcctg gggccgtcaa gcaattcaaa caggtacaaa
     1621 gcccgtatgg acggcgccca cttcgatgag aggatcagca aaagcctcat cgccgtcgac
     1681 tcgtccgtgt ggcggcccag caacagaact ttggttcctg cccatggaac cgcacattta
     1741 gctttacgtc tggcgatatc tgccctttgc tcgtcgcacc ggggctgggt aggttttact
     1801 atacaatcaa ttttcgaacc cgggcagccg cagcgcgcaa atgcctgcgg ctttttgcgt
     1861 tgcgagcgta ggctggcgaa ttgtgaagtg gcggcggtag ctcagcccgg aacgccccat
     1921 gcgcagcgca gtggcaacgg tgcgtccgcc atagcgcatc ccagccagtc taaccaccga
     1981 aaggccgccc gaaatggcgg ctttctacgt gacgagacgt gtacttgcga tctcctgtat
     2041 gatgccgccg tcagccgcat cgtctaagac agagattctc gttgatcatt gaacagatcg
     2101 gaatttgccg cgacgtactc aacccaaagc tctctcatca ttttcttcac tgcatccgga
     2161 tcgtaggcat ccccagctcg agtgaagtta ttgagctgcc cgaggcgcat gatcccatgg
     2221 ctggagtcgc ccagattctg tgcaaaacat gcgtcggtgg gcttatgcgt aatgccccac
     2281 ggcgtgagct gaaaccggaa cggcaacaaa caatagcgtc tctaaagggt atgcaacgag
     2341 gctgcgctta agccttgaaa cgcgctcggc ttgtcagagg caggtatttg acaccaagag
     2401 caaagcaatt gcggaaagac gatattctcc acacgatgac ggcttgagac atacagccac
     2461 tcaaacagtt agcggctttt cgctaaggag ccgctcacga atagcttctt ctacgagaag
     2521 aagctcccgt ttagcctcgc gcagcagggc ttcggctgac tgcgcacgga attccagtgc
     2581 ggtcacctta tcttcagccg ccgctatccg cgtttctgcg ttcatcaaag ctttcgacgc
     2641 atcttgcaat ttgcggtcag cctcgttgat aacttcgcgc cgcgcccttt cggcgacgtc
     2701 gaggcgcttt tcggcaatct gtgctctttc ggttgcactc ttgcacacgg attgtgcgcg
     2761 cgcttccgct gcccggacct gctcttccat tccactaaaa agttcggcag catgaaacac
     2821 aaggttcagc accgtcctgc cttcatcccc cggcaaagac cgagtgaaac tcagcagatt
     2881 gtcctcgctg gcggaggttg cattcatttg caatgctact ttcccaaaca ctgtcaaaat
     2941 agaaagtata acagatatgg taaagggacg gttaagtgtc cgttttggtg cggccccgaa
     3001 atgcgacttg cgcaaagtcc gcatcggctt gtcgtccgat gccgcgcgtt attaccggcg
     3061 cgcaagcagg ctcgggtcct gccttcccaa ccaactctag atcaatgcta caccgcaacc
     3121 gaacgtgaac aacgtgaacc agttgcgctg tcacgctggc gctcatcgac cagccgacca
     3181 cccggcgcaa gaacaggtag atgacagccg acacgtagag ccagccctcg gcagtccaga
     3241 tgtaggtgaa gtcggcgatc cacttctggt tcggccgttc ggtcgcaaac tgccggtcca
     3301 gaagattcgc cggcacgttc gtaacctggc gacgccttcg tccttgggca cgccgataac
     3361 cttaagattc aaaacttgat gtgcggtgcg ctcgcaacgc tatcattgct ccatgcgcgg
     3421 acgtttccag aatattttct ggttatcgct cgaccacctg atgccggacg gatcgccggt
     3481 ctttctggcg atcgtaatat tacttgtcgt gaacggggtt ttgctcgccg ccaggatctt
     3541 ttgcacgtga attgtatggc cggcaggaca ctttttccgt ttggcgatcc agccattgtt
     3601 tcgggtgcac gacaccaccg ccctcgcaga gagacgccaa gccatgaatg tgtgggctgg
     3661 ttcacataag ggtcacgagg gccgggctat cattctattg agcgaccgga gaagtcgacc
     3721 ggatggtctc gcgtgacaat catattcctc atcctttttg gaatttactc ggtaattttc
     3781 gtcgctgggc gcgcccatag ccgtcgcctc gaccgactcg agacgcgctt aaaccgagga
     3841 ccgaacgacg cacaacccac gatttgggcg ggctctgatg tcgatggcat aagctcaaac
     3901 g
//
LOCUS       DATMGX010000381         3025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_4047,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000381 DATMGX010000000
VERSION     DATMGX010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4047"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..678
                     /locus_tag="VNR65_06190"
     CDS             169..678
                     /locus_tag="VNR65_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWJ18299.1"
                     /translation="MPDRSPRDLFTEGHQRKFLVIVDESLEGETALYYAARRARRTGG
                     GLALLFVIEPPEMSHWLGVMDTFRDEQYQKARAVFRLRARKLKTWGFEDIAHEEFIRE
                     GPKAEEIVKLIEEDRDFGILVLGASTDPEGPGPLVSLLAAGAKAGTFPIPVTIVPGNL
                     SIDEIKGLA"
     gene            763..1320
                     /locus_tag="VNR65_06195"
     CDS             763..1320
                     /locus_tag="VNR65_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017532211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HWJ18300.1"
                     /translation="MFIQTEATPNPATLKFLPGKVVLDEGTREFRDTSEAEPVSPLAA
                     RLFAVDGVTGVFLGSDFISVTKDHREWQQLKPAVLGAIMEHFLSGEPVVTDGKGAAQG
                     GEDFDPKDAEIVSTIKELIETRVRPAVANDGGDIIFHGFRSGIVYLKMHGACSGCPSS
                     TATLRYGIENLLKHFIPEVEEVQPV"
     gene            1381..1974
                     /locus_tag="VNR65_06200"
     CDS             1381..1974
                     /locus_tag="VNR65_06200"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:NF003768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HWJ18301.1"
                     /translation="MAGSIAAECLNQIFLEARTHNKWQDKPVPDELLRQLVDIMKMGP
                     TSANQSPARIVFVKSAEAKERLAPHLSEGNRAKTMSAPVCAIIGYDLKFYEHLPKLFP
                     HTNAKSWFEGGPQEKIESSAFRNGTLQGAYLIIAARALGLDTGPMSGFDNAGVDREFF
                     AGTSVKSNFLCSLGYGDPSGLFSRSPRFTFEEMAKII"
     gene            2007..2684
                     /gene="tsaB"
                     /locus_tag="VNR65_06205"
     CDS             2007..2684
                     /gene="tsaB"
                     /locus_tag="VNR65_06205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWJ18302.1"
                     /translation="MKILAIETSMGRTSVALARPGKPVLVKRIESGRGQAEQLIPLIG
                     SLMEEAAVSFEALNRVAVCIGPGGFSGIRTGVAAGRGIGLAARLPVVGATSFRIMAAA
                     FEKAADVPETYGLAAPAGMSAVYCQIVARGGTAVTDIMALPQSECGSFFEGKAEVLAG
                     PAAAALVDAGFVSLPVTASDLYPDAVTLAELAPSLDPERDAPSPYYVRDADAKPQTKH
                     VIALATD"
     gene            2659..>3025
                     /locus_tag="VNR65_06210"
     CDS             2659..>3025
                     /locus_tag="VNR65_06210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ18303.1"
                     /translation="MSSPLQPIEGAAASTLEATADDAQGLAALYAEALPPGWPAHELA
                     ASCADANRAVLKAADGAHVHGFVILQFAADEAEILAIAVAKDRRRRGLAASLLRGGLR
                     ACHNRFISCIYLEVAESNTP"
BASE COUNT          593 a          923 c          913 g          596 t
ORIGIN      
        1 cgataccgcg tacatcgaca gcgtgcttgc ggacggcgcg aagcgtgcgc gcattatcgc
       61 ggacgagacg cttcatcatg tccgcaacat tgtcggcttc gtacaagcgc acaccgctga
      121 aaagcatgat aaggtaaccg gccatcgata gagcgcaatt ggaagcccat gccggaccgc
      181 tcacctcgcg atctcttcac ggaaggccac cagcgcaagt tcctggtgat cgttgacgaa
      241 tctctcgaag gcgaaacggc tttatactat gcggcccgcc gcgctcgccg gacggggggc
      301 ggcttggcgc ttctgtttgt gatcgagccg ccggagatgt cgcattggct cggcgtcatg
      361 gacacgtttc gggacgagca atatcagaag gcgcgggccg tgtttcgcct tcgcgcacga
      421 aagctcaaaa cctggggctt cgaggatatc gcgcatgagg aattcatccg cgagggccct
      481 aaggcggagg aaatcgtaaa gctcatcgag gaagaccggg atttcggcat cctcgtgctt
      541 ggcgcctcca ccgatccaga ggggccgggg ccgctcgtat ccttgctcgc ggcaggcgca
      601 aaggcaggga cattccccat tcccgtcacc atcgtgcccg gaaatctatc aatcgatgag
      661 atcaagggat tagcctgact accggagtcg gatttccact tgaaaccgct cccgaagcct
      721 gccttattag agggcgtggc atgggtgcag aaggaaattt gcatgtttat tcaaaccgaa
      781 gcgaccccga acccggcgac gctgaagttt cttccgggca aagtggtcct cgatgagggc
      841 acgcgggagt ttcgcgacac ttcggaggcg gagcctgtct cgccgctcgc cgcgcggctg
      901 ttcgccgtgg atggcgtgac gggcgttttt cttggctcgg acttcatttc cgtcaccaag
      961 gatcaccgcg aatggcagca gctcaagccg gccgtgctcg gcgccatcat ggagcacttc
     1021 ctctccggtg agcccgtggt caccgacgga aaaggggctg cccagggagg cgaggatttc
     1081 gatccgaaag acgcggagat tgtgtccacg atcaaggagc tgatcgagac gcgcgtgcgc
     1141 ccggcggtcg ccaacgatgg cggcgacatt atcttccacg gcttccgctc cgggattgtc
     1201 tatctcaaga tgcacggcgc atgctcaggc tgcccgtcct caacagccac gctgcgctat
     1261 ggcatcgaaa acctgttgaa gcacttcatc cccgaggtcg aggaggttca gcccgtctag
     1321 cgatcgcacg cgcaccggcc tatatccgtc ccaagatgaa acactcgttc gggggtatct
     1381 atggccgggt ccatcgctgc ggaatgcttg aatcaaatct tcctcgaagc ccgcacccac
     1441 aataagtggc aggataagcc cgttcccgac gagctgcttc ggcagcttgt cgacatcatg
     1501 aagatggggc cgacgagcgc gaaccaatcc cctgcgcgaa ttgtgttcgt gaaatcggcg
     1561 gaggcgaaag agcggcttgc tccgcatctg tcggaaggaa accgcgccaa aacgatgagc
     1621 gctccggtgt gcgcgatcat cggctacgac ctcaaattct acgagcatct cccgaaactc
     1681 ttcccgcata ccaatgcgaa gagctggttc gagggcggcc cgcaagagaa aatcgagagt
     1741 tctgccttcc gcaacggcac cttgcaaggc gcatatctta ttatcgcggc gcgcgcgctt
     1801 gggctcgata ccggccccat gtcgggcttc gacaacgcgg gcgtcgatcg cgaatttttc
     1861 gcgggcacga gcgtgaagtc caacttcctg tgcagcctcg gttacggtga cccttccgga
     1921 cttttctcgc ggtcgccgcg ttttaccttc gaggaaatgg ccaagatcat ctaggctagc
     1981 cgcaacgata aggcgcgatc tcagctgtga aaatcctggc aatcgagaca agcatgggcc
     2041 ggacatcagt cgcgctggcg cgacccggaa agcctgtcct tgtgaaacgg atcgagtccg
     2101 gccgaggcca ggccgagcag ctgattcctc tcatcggctc cctcatggaa gaggccgctg
     2161 tctctttcga agcgttgaac cgcgtcgctg tctgcatcgg cccaggcggc ttctctggca
     2221 ttcggactgg cgtcgctgcg gggcgtggca tcggactcgc tgcacgcctc cctgtcgtcg
     2281 gcgcgaccag cttcaggatc atggcggcgg ctttcgaaaa ggccgctgac gtgccggaaa
     2341 cctacgggct cgccgctcct gctggaatga gcgcggttta ctgccagatc gtggcccgcg
     2401 gcgggacggc ggtaaccgat atcatggcct tgcctcaatc ggaatgtggg tccttcttcg
     2461 aaggcaaagc ggaggtcttg gccgggccag cggccgcagc tctagtggac gcggggttcg
     2521 tcagcctccc tgttacggct tccgatctct atcccgatgc cgttacgctt gccgaactcg
     2581 caccaagcct tgatcccgaa cgcgatgcgc cctcgcccta ttacgttcgc gatgcggatg
     2641 cgaagcccca aacaaaacat gtcatcgccc ttgcaaccga ttgagggcgc tgcggcctca
     2701 accctggaag cgaccgccga cgacgcgcaa ggtctcgcgg ccttatacgc cgaagccttg
     2761 ccgccgggtt ggcccgctca tgaactcgcc gcctcctgtg ctgatgcaaa ccgggccgtt
     2821 ttgaaggctg cggatggcgc gcatgtgcat ggcttcgtca ttctgcaatt cgcagccgac
     2881 gaggcagaaa tcctagcgat tgcagtcgct aaggaccggc ggaggcgcgg gcttgcggca
     2941 tctttgctcc gaggtggctt aagggcttgc cacaaccgat tcatctcttg catttatctg
     3001 gaagtggcag aaagtaatac ccccg
//
LOCUS       DATMGX010000382         3704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_243988, whole genome shotgun sequence.
ACCESSION   DATMGX010000382 DATMGX010000000
VERSION     DATMGX010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243988"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VNR65_06215"
     CDS             <1..382
                     /locus_tag="VNR65_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HWJ18304.1"
                     /translation="QWYFALGAEYAWSPSLTVRTGVNYEISPVDNSNRTILLPDSNRL
                     GVSVGGSYRYSDRVTFDLAYTHLFFDDAPFCMANGGGTRHCISSVPQTLLLQGSANTS
                     VDMFSFAAKYSTGAPVAALEPYKK"
     gene            527..1333
                     /locus_tag="VNR65_06220"
     CDS             527..1333
                     /locus_tag="VNR65_06220"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HWJ18305.1"
                     /translation="MTRYKAYVRYRAIFLSDIHLAARACRSRDLLSFLKSHDAPVIYL
                     VGDVIDFWRLRRGPNWRPSQTEVVMELLAKAQNGTRIIYIPGNHDEELRILAGITLGP
                     LEIRLRAIYDTADGRRFLVIHGDEFDTVVNKAHWLAVIGDIGYDIAWVANEVLNAVRR
                     SLGLSYWSLSAYLKYSVKRIVNYAINFETSLAAEARRVGASGVICGHIHHASARLIED
                     IQYVNTGDWVDSGTAVVEHFDGRMEIVRWTDHLRALQASASKVPVASVTS"
     gene            1333..2394
                     /locus_tag="VNR65_06225"
     CDS             1333..2394
                     /locus_tag="VNR65_06225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWJ18306.1"
                     /translation="MRILIATDAWKPQVNGVVNTYSNLEREGAKDGLDLTFLTPSGFR
                     TLPLPTYSEIRLAVIRPRDVARRVEEDSPDFVHIATEGPIGLAARAYCRDAGRRFTTS
                     YHTRFPEYVSARMPIPLSWGYMFERWFHNAGSGVMAASLSLCHELTAQGLKNVHLWSR
                     GVDLDLFKPRTVDRFGVPRPLFLYAGRVAIEKNLEAFLNLDLPGTKAVVGGGPQLEEL
                     KQRYAQAVFTGPKFGQDLAEHYASADVFVFPSLTDTFGNVLLEALACGVPVAAYNVMG
                     PKDVVANGSVGILGKDLKESALAALELDRRECRSYAELFSWAACARRFSEIIAEANGV
                     QHASQFRNETDDIALGTLR"
     gene            2409..3410
                     /locus_tag="VNR65_06230"
     CDS             2409..3410
                     /locus_tag="VNR65_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HWJ18307.1"
                     /translation="MDTPTIDAIADADLHIRPYAIETPLLEWGALNEKLEARVLCKAE
                     LLQRTGSFKFRGAYNRVSRIDRSAFPGGVVACSSGNHAQGVAEAARLCGIDAVIVMPS
                     DAPKVKIARTSHCGAEIVFYDRDTEDRSAIARDLCEKLKADFVAPFEDPVVVAGQGTV
                     GLEMTRQAKAAGAEIDAVLVPISGGGLTAGVAIAVKDQFPKAEIYCVEPKGFDDYRQS
                     LASGTRQKNEGTSRSICDALLMPEPGELTFAINKDLLAGGLVVSDADVRHAVSYAWRD
                     MKLVVEPGGAVALAALLSGAFEAKGRNVAIVLSGGNVDPHVFADIICNIKELVISGQ"
     gene            3494..>3704
                     /locus_tag="VNR65_06235"
     CDS             3494..>3704
                     /locus_tag="VNR65_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18308.1"
                     /translation="MIGAANALAVEPGFSLILKEWTVEHPDWPAAAPSLRIGILTDIH
                     AVEPWMSAHRIGGIVDQLNAQKPDVI"
BASE COUNT          750 a         1062 c         1085 g          807 t
ORIGIN      
        1 tcagtggtat ttcgcactag gcgcagagta cgcttggagt ccctcgctca cggtgcggac
       61 gggcgtcaat tacgagatct caccggtcga caacagcaat cgcaccatcc tgttgccgga
      121 ctccaatcgc ctcggcgtta gcgtgggcgg ctcttacaga tattcggatc gcgtcacttt
      181 cgatctcgcc tacacccatc tgttctttga cgacgcccca ttctgcatgg ccaatggcgg
      241 cggtacgcgg cactgcattt cttctgtccc gcagaccttg ctactgcagg gctcggctaa
      301 cacttccgtg gatatgttct cgtttgcggc gaagtacagc acgggcgctc cggttgcagc
      361 gcttgagccc tacaagaaat aagcgaacag ccacacacaa aacgctgggc tggcgaaagc
      421 tggccctttg tgttttcgga ggcttgtcat actcctgaaa ccttgattgc tgcgtctatt
      481 catcctggca cttaaggggt cgaggtgaag ggtggatcac agggcaatga cgcgatataa
      541 agcgtatgtg agatatcgtg ccatcttcct ttctgacatt cacctcgccg cgcgagcttg
      601 ccgctcccgt gatttgctga gctttttgaa gtcgcacgac gcccccgtca tctatcttgt
      661 cggggatgtt atcgactttt ggcggctcag gcgcggtccg aactggcgtc cttcgcagac
      721 ggaggtcgtg atggaactgc tggccaaggc gcaaaacggc acccgcatca tctacattcc
      781 cggcaatcac gacgaggaac tgcggatttt ggccggcata acattgggcc cgctcgagat
      841 tcgcctgcgt gcgatatacg ataccgccga cggccgccgc tttctggtca tccacggcga
      901 cgaatttgac acggtcgtga acaaggccca ttggctggct gtgattggcg atatcggcta
      961 cgacattgcc tgggtcgcca atgaagtctt gaacgcagtc cggcgcagcc tgggcctttc
     1021 atattggtcc ctgtccgctt atctcaaata cagcgtcaaa cgtatcgtga attatgcaat
     1081 caatttcgaa acttcgcttg ctgcggaagc acggcgggtg ggagcctcag gggtgatctg
     1141 cgggcatatt catcacgcct cagcgcggct gatcgaagat attcaatatg tgaacaccgg
     1201 cgactgggtc gacagcggca ccgccgtggt cgagcacttc gacggccgga tggagattgt
     1261 ccgctggacc gatcatcttc gcgcgcttca agcgagcgct tccaaggtgc cggtcgcttc
     1321 agtcacatcc tgatgcgtat cctgatcgca acagatgctt ggaaaccgca agtcaatggc
     1381 gtcgtcaaca catacagcaa tctcgagcgc gagggcgcga aggacggact ggatctgaca
     1441 ttcctgacgc cgtcggggtt cagaacgctt ccgcttccca cttacagcga aatccgcctc
     1501 gccgtgatcc gccctcgcga cgtcgcaagg cgcgtcgagg aagacagccc ggacttcgtt
     1561 cacatcgcga ccgaggggcc tattgggctt gcggcgcggg cttactgccg tgacgccgga
     1621 cgccggttca cgacaagcta tcacacccgg tttccggaat atgtctcggc tcgcatgccc
     1681 attccactat cctgggggta catgttcgag cgctggtttc ataacgccgg ctctggcgtg
     1741 atggctgctt ccctttctct gtgccacgaa ctgactgcgc agggtttaaa gaatgtgcat
     1801 ctttggtcgc ggggcgttga tctcgatctt ttcaaaccgc gcacggtgga tcgcttcggc
     1861 gtaccccggc ccctcttcct ttatgcgggg cgggtagcca ttgagaagaa cctcgaagct
     1921 ttcctcaatc tcgatcttcc agggacgaaa gccgtggtcg gaggcggccc gcagctcgaa
     1981 gagctaaagc agcgctatgc gcaagcggtg tttaccggcc cgaaattcgg acaggacctc
     2041 gcggagcatt acgcttcggc cgacgttttc gtgtttccga gcttgaccga tacctttggc
     2101 aacgtgcttc tggaagccct ggcgtgcggg gtgcccgtgg cggcatacaa cgtcatggga
     2161 ccgaaagacg ttgtggcgaa cggcagcgtc gggatccttg gaaaagatct taaagaatca
     2221 gccctggccg ccctggaact tgatcgaagg gaatgccgct cgtatgcaga gctgttcagc
     2281 tgggcggctt gcgcgcgccg cttttctgag atcatcgccg aggctaatgg cgttcagcat
     2341 gcatcccaat ttcgtaacga aacagatgat attgcgttag gaactctgcg ataacccacg
     2401 gaaaaccgat ggatacgcca acaattgacg ctatagcgga tgcagacctg cacattcggc
     2461 cgtatgcgat tgaaacgccc cttctcgaat ggggagcgtt gaacgaaaag ctggaggcaa
     2521 gagttctttg caaggccgaa ctgctgcaaa gaacgggaag tttcaagttc cgcggagcct
     2581 ataatcgcgt ctcgcgaatc gaccgctcgg cgttccccgg gggggtcgtt gcctgctcgt
     2641 ccggcaatca tgcccaaggc gtcgccgagg ccgctcggct ttgcggcatc gacgccgtga
     2701 tcgtgatgcc gtcggatgcg cccaaagtga aaatcgcacg caccagccac tgcggcgctg
     2761 agatcgtgtt ctatgatcgc gatacagagg atcgcagtgc catagctcgc gacctctgcg
     2821 aaaagctgaa agccgacttc gttgcgccct tcgaggatcc cgtcgtggtc gcggggcaag
     2881 gcacggtggg tctcgaaatg acgcgccagg cgaaggcggc aggcgccgaa attgacgcgg
     2941 tgctcgtgcc gatcagcggg ggaggcctga cggccggggt tgcgatagcc gtcaaggacc
     3001 agtttccaaa agccgaaatc tactgcgtcg agccgaaagg ctttgacgat taccggcaat
     3061 cgctggcttc aggaacgcga cagaagaacg aaggcacgag ccgctcgatc tgcgacgctc
     3121 tgctgatgcc ggagccaggc gaactgactt tcgctatcaa caaggattta ctggcaggcg
     3181 gcctcgtcgt cagcgacgct gatgttcgac atgccgtgtc ttatgcttgg cgcgacatga
     3241 agcttgttgt cgaaccagga ggtgcagtgg cccttgcagc tctactctca ggcgcctttg
     3301 aagccaaggg gcgcaatgta gccatcgttc tttccggcgg gaacgtcgat cctcacgtct
     3361 ttgccgacat tatctgcaac atcaaggaat tggtgatatc aggccagtaa gcggtaaggt
     3421 tgaggagtta cgcttcggca tgatctcacg acgaaacttc cttaccttga ctgctgcgac
     3481 ggccggagcg ggcatgatcg gcgcagcaaa tgctctcgcg gtcgaacctg gtttcagtct
     3541 cattctcaaa gagtggactg tggagcatcc cgactggccc gcagctgctc cctcactgcg
     3601 catcggcatc ttgaccgata ttcatgcggt tgagccatgg atgtctgcac atcgcatcgg
     3661 cggcatcgtc gatcaactga atgctcagaa gcccgatgtc attg
//
LOCUS       DATMGX010000383         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_295237, whole genome shotgun sequence.
ACCESSION   DATMGX010000383 DATMGX010000000
VERSION     DATMGX010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295237"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..1913
                     /locus_tag="VNR65_06240"
     CDS             join(502..850,850..1913)
                     /locus_tag="VNR65_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612190.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HWJ18309.1"
                     /translation="MPAPAAAAPELSDRPRRRTFTVETKLRVLAETDAAADVGGIGAI
                     LRREGLYSSILTDWRRLRDSGALGALTPAKRGPKRAVLNPLTAELAQARRENARLGRR
                     LEHAEAIIEVQKKVGGTVGYPAGDARQRRRVMMDAVVALAPTPGLTASACAALNVSRA
                     SVYRQRVRLARPLAARCRRPSPQRALTVVERQTVLDLLRAPRFADQAPAEVYASLLDE
                     GSYHCSIRTMYRILAASQEVRDRRDHLRHPIYKKPELLAQAPNEVWSWDITKLMGPAK
                     WTYFYLYVIIDIFSRRIVGWCVADRESATVFTTLFNDATAKHPAPPGQLTLHADRGGP
                     MRAKATALLLADLGVTKSHSRPHTSNDNPFSESHFKTMKYQPQFPQRFGCIQDAKAFC
                     RNFFDWYNQEHHHAGIGLMTPDQVHYGQADAIHAARQNILDGAFNRNPERFVRKEPEP
                     PAKPIATWINPPPKTLNIQA"
BASE COUNT          587 a          809 c          792 g          441 t
ORIGIN      
        1 atcaccatag tgggcatccc cttcgcttgg gcgcacctga aacttgctgg aatagcgctc
       61 tggcctatcg gcaagatgat cgtgccggcc taggaaaatc cgcgccgatg ccgagtaggc
      121 ccgaggaatt tcgccccggg cctctcacag atccggactt gatactctcg catcatccgg
      181 ctcgtatcat cgcaggaagg ctgccgcatt ccgctgaaac gtcaggctcc tcccggtttg
      241 acccggttgg cccaactcag ccgcgatgac ccgccccctt cgctccacgg gcattacccc
      301 gcttcatcac tactacagag cagtccgtcc ctctccggcg catcggtacc cctccgtgcc
      361 aatgatggtg agacatcgga gtcccgaaag tttagcctca catgggcgta ggacaaccat
      421 cacgtggtca tgcaaaacaa agaagcgaaa acggacggtg ccgccggggc tggtacggag
      481 ggaggccgga ggccgaccgc agtgccggcc ccggcagcgg cggcgcccga attgtcggat
      541 cggccgcggc gtcggacttt tacggtggag acgaaactgc gtgttctggc agagactgac
      601 gccgccgccg acgttggtgg gatcggagca atcctgcgcc gagaaggctt gtactcatcg
      661 atcctgacgg actggcggcg attgcgcgac agtggagccc tcggtgcgct gacgcccgcc
      721 aagcggggac ccaagcgcgc ggtgttgaat cctttgacgg cggaactggc gcaggccagg
      781 cgggagaatg cacggctcgg gcggagattg gagcacgccg aagcgatcat cgaagtccaa
      841 aaaaaagttg cggcactgtt gggtatcccg ctggcgacgc cagacagcga cgacgagtca
      901 tgatggatgc agttgttgcc ctggcgccga cacccggtct gaccgccagt gcctgcgcgg
      961 ctctcaacgt gtcgcgcgcc agcgtctacc gtcagcgcgt ccggctggcc cggccgttgg
     1021 ccgcgcgttg tcgcagaccg agtccacagc gtgcgctgac cgtcgtggaa cgacaaaccg
     1081 tgctcgacct tctgcgcgcg ccgcgttttg ccgatcaggc gcccgccgaa gtctacgcca
     1141 gcctgctcga cgagggctcc taccactgct cgatccgcac catgtaccgc atcctggccg
     1201 caagccaaga agtccgcgat cgccgcgatc acctgcgcca tccgatttac aaaaagccgg
     1261 agctgctggc gcaagcaccc aacgaggtct ggtcgtggga catcacgaag ctgatggggc
     1321 cggccaaatg gacgtacttc tatctctacg tcatcatcga tatcttcagc cgcagaatcg
     1381 tcggctggtg cgtcgccgac agagaaagcg ccaccgtgtt cacgacactg ttcaatgatg
     1441 ccaccgccaa acaccccgcg ccgcccggcc agttgaccct gcatgccgac cgtggcggtc
     1501 ccatgcgcgc caaggccacc gcgctcctgc tcgccgatct cggcgtcacc aaatcccaca
     1561 gtcggccaca cacctcaaat gacaatccgt tctcggaaag ccacttcaaa acgatgaagt
     1621 accagccgca atttccacag cgcttcggct gcattcagga tgccaaggcg ttctgccgca
     1681 acttcttcga ttggtataat caggagcatc atcatgccgg catcggccta atgacgcccg
     1741 atcaggtgca ttacggtcag gccgacgcaa tccacgccgc tcgccagaac atcctcgatg
     1801 gcgccttcaa cagaaacccg gaacgcttcg ttagaaagga gcccgaaccg cctgcaaagc
     1861 caatcgcgac ctggatcaat ccaccgccga aaacgctaaa catccaagct taaattccac
     1921 actgggctgt ctcaaaatcg ttgacacgtt ccgctgggca gcgcgtgagt catgggggtt
     1981 gaagtcccct tggcgctcgg atgaggagcg ctgtatccga aggcgggggt aattcctcgc
     2041 tggccgggag agcaagaagg tatggagaag tctaaacggg cacgaaggcg aaagccggac
     2101 acagccaaga cggacctgaa gccagttgct cagagtaacc cggagggcag ggtgtcagga
     2161 gcgatcctga atccgaaggg tctgaggaga agtatcgggc tgtaatcaat gggggccacc
     2221 ccgaagatga acaggtcggc caaagatggg gcaaggtcga gcccgcacta tgggggcgaa
     2281 ggcgttactc attcacccat cgtaccaagg tgtttgctga cggcacgata cggaagattc
     2341 atgcgtgacc ccaggagacc tgcatgggtc ccggcccaaa gccgggtaag cgaccctata
     2401 agcgaaagcg aaatgggaag cgaagcacat gcaggagtcg gagaacgtaa tagtacctga
     2461 agcgcgagac gaacaactcg cgtgaccgga aacggcggat cgtgagtgat ccggggaagg
     2521 acgttcgtga acggtttgag ccaggactaa tcgaggcaga tgagaaagga gcgtgaagat
     2581 ggaaagttcc ctatgtgcaa aacccgaacg tcacgctccg aggagactg
//
LOCUS       DATMGX010000384         2970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_176259, whole genome shotgun sequence.
ACCESSION   DATMGX010000384 DATMGX010000000
VERSION     DATMGX010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176259"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..2179)
                     /gene="rpoD"
                     /locus_tag="VNR65_06245"
     CDS             complement(59..2179)
                     /gene="rpoD"
                     /locus_tag="VNR65_06245"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWJ18310.1"
                     /translation="MATATTKTEDQETPASETTDSPVLDLLDAAVKRLIKAAKVRGYV
                     TYDEINEVMPPEEVSPDQIEDIMTMFSDLGINVVDADEVEEAAAAEPIDEDEEDRHAP
                     QPHLPVKQERSTEISERTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGREAMIGGL
                     CESPLTFQAIIIWRDELNEGKILLRDIIDLEATYSGPFVRTGEGEEEGAEEDFVPDEP
                     PAPAPAPAPAAPNGASRTAAPGMNGANGHAHPRPADGEPAQSGDGEGESAEFRGEENY
                     DEDDDLENSLSLAAMEAELKPKVLETFDRIATEYKKLRKLQDQHVERKFQDDSLSPQQ
                     EKRSRKLRDAVIQDVKSLSLNPARIESLVEQLYDINKRLIGYEGRLLRLADGYGVRRD
                     EFLGQYQGTELDSSWVDRVADLKGKGWQKFVEKERARIDEIRREIYALATETGLDIGE
                     FRRIVKIVQKGEREARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKA
                     VDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIVRTSRQMMHE
                     IGREPTPEELAEKLSMPLEKVRKVLKIAKEPISLETPIGDEEDSHLGDFIEDKNAILP
                     IDAAIQSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQ
                     IEAKALRKLKHPSRSRKLRSFLDN"
     gene            complement(2442..2855)
                     /locus_tag="VNR65_06250"
     CDS             complement(2442..2855)
                     /locus_tag="VNR65_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18311.1"
                     /translation="MALARHPWLLDQHLDEIASLHLEDAGCCRIRNAMLTIHQTEELL
                     DNKKLLEHLSRDSYGAELERLERACAHNADPHFASGANKEQVLEGWQHVMMLHGKAGV
                     PRSLREAESDYVSEPTNENFSKLRAVVQQVEIAVS"
BASE COUNT          530 a          906 c          833 g          701 t
ORIGIN      
        1 agtgcaaata aaagccaagc ttaagcgcta attctcgata attgccagcg atgcagactt
       61 aattatccaa gaagctgcgc agtttccggc tgcggctcgg atgcttcagc ttgcgaagcg
      121 ccttcgcctc gatctggcgg atgcgttcgc gtgttaccga gaactgctgg ccgacttctt
      181 ccagcgtgtg atcggtattc atcccgatgc cgaagcgcat acgcagcacg cgttcctcgc
      241 gcggcgtgag cgaggcaagc acccgtgttg tcgtctcgcg caggttggac tggatcgccg
      301 catcgatggg caggatcgcg ttcttgtcct cgatgaaatc gccgaggtgg ctgtcttcct
      361 cgtcgccgat cggcgtctca aggctgatcg gctccttcgc gatcttcagc accttgcgca
      421 ccttttcaag cggcatggac agcttttcgg ccagttcctc cggcgtcggc tcgcggccga
      481 tctcatgcat catctgccga ctcgtgcgca cgatcttgtt gatcgtctcg atcatgtgca
      541 cgggaatgcg gatcgtgcgg gcctgatcgg caatggagcg cgtgatagcc tgccggatcc
      601 accatgtcgc gtaggtcgaa aacttgtatc cgcgccggta ttcgaactta tcgaccgcct
      661 tcatcaagcc gatattgccc tcctggatca aatccaggaa ttgcagaccg cggttcgtgt
      721 atttcttggc gatggagatc acgagacgca aattcgcctc caccatctcc ttcttcgcct
      781 ggcgggcttc tctctcgccc ttctgcacga tcttcacgat gcggcggaac tcgccaatgt
      841 cgaggcccgt ctcggtcgcg agcgcgtaga tttctcggcg gatctcgtcg atgcgggccc
      901 gctctttttc aacgaatttc tgccagcctt tgcccttcag gtcggcgacg cggtcgaccc
      961 agctcgaatc gagctcagtg ccctgatact ggcccaggaa ctcgtcgcgc cgcacgccat
     1021 agccgtcggc caggcgaagc aggcggcctt catacccgat aagccgctta ttgatgtcgt
     1081 agagttgctc gacgagtgac tcgatacggg ccgggttcaa ggacaggctt tttacgtcct
     1141 gaatgaccgc gtctctcagc ttcctcgacc gcttctcctg ctgaggcgaa agcgaatcat
     1201 cctggaactt tcgttcgaca tgctgatcct gcagcttccg aagcttcttg tattcggttg
     1261 cgatccgatc gaaggtttca agcaccttcg gcttgagttc tgcctccatc gccgcgagcg
     1321 agaggctgtt ttcgagatcg tcatcctcgt cgtaattctc ttcgccgcga aactctgcgc
     1381 tctcaccctc gccatcgccg ctctgagcgg gttccccgtc cgcagggcgc ggatgggcat
     1441 gtccgttggc gccgttcatc ccaggcgctg cggtccgcga ggcgccattt ggcgccgctg
     1501 gagcaggggc tggagccgga gccggaggct catcgggcac aaaatcttcc tcggcgccct
     1561 cttcctcgcc ctcgccagtc cgcacgaacg gacctgaata ggttgcttcc aggtcaatga
     1621 tgtcgcggag gaggatcttc ccctcgttca gttcatcgcg ccaaatgatg atggcttgga
     1681 aggtgagtgg gctttcgcac aggccgccga tcatggcttc gcggccggcc tcgatgcgct
     1741 tcgcgattgc gatctcgccc tcgcgcgaga gaagctcgac cgagcccatt tcgcgcagat
     1801 acatgcgcac gggatcgtcc gtgcgttcgc ttatctcggt tgagcgctcc tgcttgaccg
     1861 gcaaatgagg ctggggcgcg tgcctatctt cctcgtcctc gtcgatgggc tccgctgcgg
     1921 ccgcttcttc gacctcatcc gcatcgacga cattgatgcc gaggtcggaa aacatcgtca
     1981 tgatgtcctc gatctggtcg ggagagacct cctcgggcgg catgacttcg ttgatctcgt
     2041 cgtaggtaac gtaaccgcgt accttggcgg ccttgatcag ccgttttacg gcggcatcca
     2101 aaagatcaag aaccgggctg tccgtcgtct cggaagcggg ggtctcttgg tcctctgttt
     2161 ttgtcgtcgc tgtggccatt atgcactcca gaaaatgccg aaaaacagtc cgcgcaggca
     2221 gacgggcggc cttggcttaa ggtagcggtc tatctcctgg ggcgccgtaa cagtaaagcg
     2281 ttgctcgaca cggctttcgc gattcgcttt gctaaaccac cttaaatgat aagtggttgt
     2341 gcttaatgcc gcgttactca tgaagctaga atcacgcact accgcgcgcc ccgtatacta
     2401 tctggtgaat gtttgcccca ggcgcaatat caaaggcccg atcaagatac cgcaatttca
     2461 acctgttgca cgacagcacg gagcttcgaa aagttctcgt ttgtcggttc gctcacgtaa
     2521 tcactctcag cttcacgcaa tgagcgagga actcccgctt tgccatgaag catcatgaca
     2581 tgctgccaac cttccaagac ctgctcttta tttgcccctg acgcgaagtg cggatcagca
     2641 ttatgagcgc acgcccgctc aagccgttcc aattccgctc cataactgtc acgggacagg
     2701 tgctctaaga gctttttgtt gtcaagcagt tcctctgtct gatgtatggt tagcatcgcg
     2761 tttcgtatgc ggcaacagcc tgcatcttcc aaatgaaggc tcgcgatctc gtccaggtgc
     2821 tggtcgagaa gccaaggatg gcgggccagt gccacgatga gcagcgcctc tctcgccgca
     2881 ctgcacggcg ctgatggttg cgcgattgga ctggcaagaa ggctgctcgt gcgcgctgcc
     2941 gcgttcggct tcgcgtatcg cgaccgggac
//
LOCUS       DATMGX010000385         3003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_376070, whole genome shotgun sequence.
ACCESSION   DATMGX010000385 DATMGX010000000
VERSION     DATMGX010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376070"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1129..1956)
                     /locus_tag="VNR65_06255"
     CDS             complement(1129..1956)
                     /locus_tag="VNR65_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWJ18312.1"
                     /translation="MSYAALLVYVDIDGLPEQRVRIASELAAKFDAALIGFSARAVRP
                     PFVAEEAIIELTTEAADIKQMRKALAKRGNWFRRIVGTDRQDVEWRTTLDLPCDALAR
                     EARCADLLIIGQQTEGRHDHHRTLDPGEVLLRLGRPALVVAPGVASLRAEHVVVAWKD
                     TREARRAVLDAVPFLRAAKRVSVIEVCSRDEERNAVARIGDVVRYLSRHRITAESRPI
                     LHRDRSDADHLLQFALDEDADLLVAGAYGHSRLGEWIYGGMTRDILASSPLCCLMSH"
     gene            complement(2052..2387)
                     /locus_tag="VNR65_06260"
     CDS             complement(2052..2387)
                     /locus_tag="VNR65_06260"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ18313.1"
                     /translation="MAHTLVPSDHVETASVYGRGDEKIGTIERLMLEKKSGTVAYAVV
                     RCGGLLKGEVRHYPVPWDSLKYDVARKAYTTNLTLEELRSGPSELDGEAFDWGDRSAT
                     YRHPQYWSV"
BASE COUNT          628 a          918 c          863 g          594 t
ORIGIN      
        1 ccgccgcgcc agtccgatac cggctacaag cggcccaaga agtcgaccca gcgtttcgtg
       61 ccccgggtgt attgagctgg acgctgaccg tctcccctcc ccccgcgaag cggtggggag
      121 gggtcggggg tggggggtgc ctcggcaaac tcgctgccaa agcagtgaac tgatagaccc
      181 cccgacccct ccccgccacg cgcaagggcg cgtgggtgga ggggagctca agagctatgc
      241 tcgcttgtcc gggacgacgg agccattttc cgattcgaat ttcaaacagc ggatgtgcgt
      301 ccgcattctc gcggcacgat tttgcccgag ctttgcgtat cgttgtccct ccaaagaggg
      361 agcagggaat gccggatgcg cgctgcaccc gcggtctcgt gtgcaaagat gtgcgaaaaa
      421 aaacgcacac gagcatacag gttcagcgga ggcactccgg cattccctgc gcaatggctt
      481 tacggcttat gacgcgctcc ccggagaatc gggctttgtt gtctccgtcg tctgcgaata
      541 gctggcgcat ccgcgcccgg tcgggccgac tcgccttttc gccgacttga cgccaaccgc
      601 tgaggcgtca ggaccacacg actcacgact tcaccgtacg cttcggcgcc gctcgtcagc
      661 acgctccggg atcgctcacg agccctgaaa gctcgccctg ccatcccatc gcgcgcacga
      721 cgccgccgcg tccaccgcat cccgtcccaa cgttcgtgac gatggccaac gccccttcct
      781 tggggacggg atgtcgggag ctttaaaagt gatttgcccg gcgatcaaag cggaaatctg
      841 cccgacgggt tgttttgtcg cagccgcggc ggccatcaag gcatgaacgc atggattcgg
      901 tattggacgg gcgtccgctt tgctatgcgt tccggactca cgtcggacat cgcgtgatgt
      961 ccgaaatgtg ccatgtgccg gtggaggagc cgtccacagc atcaacaaga gacatggccc
     1021 ctgttcgaaa tggaacccat caacattgag gaggaatgca atgaagcatc ctcgcaaaat
     1081 ttcagcggca gacgccgctg caatagtccg cagcgaggta cctgcgacct aatgagacat
     1141 gaggcagcaa agggggcttg aggcgagaat atcccgcgtc ataccgccgt agatccattc
     1201 gcccaaccgg ctgtgaccgt aggcgccggc caccagcagg tcggcatcct cgtccagggc
     1261 aaactggagc agatgatctg cgtcagacct gtcgcgatga agaattggcc gagactctgc
     1321 agtgatccgg tgccgcgaga gataacggac aacgtcccct atccgggcta ccgcatttcg
     1381 ttcctcgtcc cgggagcaaa cttcgatgac cgaaacgcgc ttggcggcac gtaggaacgg
     1441 gaccgcatcc agcacggctc gacgggcctc gcgggtatcc ttccaggcca cgacgacatg
     1501 ttcggcccgc aacgaggcga cgcccggcgc aacaaccagc gcgggccggc ctagcctgag
     1561 aagcacctca cccggatcga gggttcggtg atggtcatgg cggccctcag tctgctgccc
     1621 aatgatgagg agatcggcac agcgggcttc ccgcgccaat gcatcgcacg gcaaatccag
     1681 cgtcgttcgc cattcgacgt cctgacgatc cgtgccgacg attctgcgga accagtttcc
     1741 tcgctttgcc agcgccttgc gcatttgctt tatgtcagca gcttccgtag tcaactcgat
     1801 gatcgcctct tctgcgacga atggcggacg aacggcacgg gcggagaaac ctatcagcgc
     1861 tgcatcgaat ttcgcggcaa gctccgaggc aattcgaacc ctttgttccg gaagaccatc
     1921 gatgtcgaca tagaccaaca gggctgcata ggacataagg ccgctccaca aaagcaaagc
     1981 catgttcgca ctgcaccgcg accagctttc ttgctggggc agtggcatac actcgcggca
     2041 cgttggctga tctacaccga ccaatactgc ggatggcgat aggtcgcgga gcgatcgccc
     2101 cagtcgaaag cctcgccgtc gagctccgat gggccgctcc gaagttcttc taacgttagg
     2161 ttggttgtgt aggccttacg cgccacatcg tacttcagcg aatcccaagg caccggatag
     2221 tggcgtacct cacctttgag taatccgccg catctcacga ccgcgtaggc aactgttccg
     2281 ctcttttttt caagcatcag tcgctcgatc gtgccgattt tctcatcgcc gcgaccgtag
     2341 accgatgcag tctctacgtg atcgctggga accagggtat gagccatggc cacctccctg
     2401 catcttcgcc accgaacatt cacgctcggt agacccacaa aaaagtttgc attaatcgga
     2461 tcatcggcaa ttgatgtgga tcaacggccc tgggtgacat cacctggagg ttattttaag
     2521 actttggctt tcggcttttg gaaggaaccc gagacgggtc acgcagaaga tgtttagagg
     2581 tcgccgaggt ctgctccgga gtcacaatgc gaaatactcg cactgagcag atgtttttcg
     2641 ctttgcctcc aaactcagac attgcttgat gctgtcgaca cttcgcattt tgccacttcc
     2701 ggactcatgc accgcagcaa atggaactct tatacgtcgg tgggcaaagc agcgttgtta
     2761 cgggcgagtg ctgagaggct ccgaagccga cggggaaatc ggaccgcgca agtctggatt
     2821 cgttcgataa ttgggataag gttgcgctac ttgcgcagaa aaaaatcaaa acgacccacg
     2881 ggaggaaatg atgctcgaca ccacgcttaa cgcgcgcgtc caagtttttc tcgacaaatt
     2941 cgacgcctcg cttgtggcgg gcgatctcga cgccgccatg ggaatgttcg ctccggaatg
     3001 cta
//
LOCUS       DATMGX010000386         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_295135, whole genome shotgun sequence.
ACCESSION   DATMGX010000386 DATMGX010000000
VERSION     DATMGX010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295135"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1584)
                     /locus_tag="VNR65_06265"
     CDS             complement(79..1584)
                     /locus_tag="VNR65_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWJ18314.1"
                     /translation="MTRYPWWRKHKEDGSHASRYPWWRHHRQASTSDGPRVPHPGGDG
                     GRQYPHLPRDPGSYDIWWKFRGPILPPPPVALAPAIPVPPAPVAVIGSGAAATAIAGF
                     GGSRPPIAPRFSRGQGREPSSAVAALLNNKRHRAKELLIEVATGSPDQLKRALANQYG
                     VEVRELGSIELINARIWHLTLVRGQALATVLGQLLQDSRVISAQPNYVYTPVQGAGQG
                     SPAAYSETALPASPVEALPTGGGVKVAIIDTCVDRNHAEIQGSVQSFFDATPSGSQAC
                     AAENHGTAVASLIGGHDQIRGSAPGAELMEARAFSETEEEKEVAATSREIALALDWAA
                     RQGARVANLSFAGPSDPLVERAVAAAYTKGIVLVGAAGNAGPSSEPLYPAAYPEVIAV
                     TATDAKRALYGAANRGSHIAVSARGVDVIVAHVNNGYGTDSGTSFAAATVSGIAANLI
                     KMRPRATPDEIRAALQGTAISPSGQGKDELFGYGLVNAQAATSFLEASVSQ"
     gene            complement(1929..>2175)
                     /locus_tag="VNR65_06270"
     CDS             complement(1929..>2175)
                     /locus_tag="VNR65_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18315.1"
                     /translation="TASGPASNAAAKRAFIVSFSQTATMAEINTLLDDAGASIVEGPS
                     ADALYHLAVPGDTVEAQEIAYRKLRASPAVKLVLPEK"
BASE COUNT          400 a          673 c          692 g          410 t
ORIGIN      
        1 gattataaag tgctagctag agcagcttat ccgcgtccaa acggacgcgg gctgctctgg
       61 gtcgagcgga acccgtggtt actgcgaaac gctggcctcc agaaacgagg tcgcagcttg
      121 ggcattcaca aggccgtatc cgaacaactc gtcctttccc tggccggatg ggctgatggc
      181 agtgccctgc agcgcggcgc gtatttcatc aggcgttgcg cgcgggcgca ttttgatgag
      241 attcgcagcg atgccgctga cggtcgccgc agcaaaggaa gtgccggagt cggtgccata
      301 tccgttgttg acatgcgcga cgatgacatc gacgccgcgt gccgaaacag cgatatgcga
      361 gcctctgttg gcagcgccgt aaagcgcgcg cttggcgtcg gttgccgtta ccgcgatcac
      421 ttcgggataa gccgccgggt aaagcggctc ggacgacggc cccgcgttgc ccgcggcgcc
      481 cacaagcacg atgcccttcg tataagcggc cgcaaccgca cgctcgacga ggggatcgct
      541 tgggccggcg aaactcaggt tcgcgacgcg cgcgccttgc ctcgccgccc agtccaaggc
      601 cagcgcaatc tcccggctgg ttgcggcgac ctctttctcc tcctccgtct cgctaaaggc
      661 ccgcgcttcc ataagttcag cgcccggagc ggagccccgg atctgatcgt gccccccaat
      721 caggctcgca acagcggtcc cgtggttctc agccgcgcaa gcttgcgatc ccgatggtgt
      781 cgcgtcaaag aaggactgca ccgacccctg aatttcggcg tgattgcgat cgacgcaggt
      841 gtcgataatg gcgaccttca ccccgccgcc cgttggaagc gcctcgacgg gcgatgccgg
      901 caaagccgtt tcgctatacg cggcgggcga accttggccc gctccttgaa cgggggtgta
      961 gacgtaattc ggctgagcgc tgatgacgcg actatcctgc agcaactggc cgagcaccgt
     1021 cgcaagagct tgccctcgga caagcgtgag atgccaaatg cgcgcgttga tcagttcgat
     1081 cgagccaagt tcgcgcactt cgacgccata ttggttcgcg agagcccgct tgagctgatc
     1141 ggggctgccg gtcgcgactt cgatcagaag ttccttggcc cgatggcgct tgttgttgag
     1201 gagggcggcc actgcggagg atggctctcg accctgcccc cgcgagaacc gcggcgcgat
     1261 cggcggacgc gatccgccaa aaccagcgat ggcggtggcc gcggctccgc ttcctatcac
     1321 ggcaactgga gccggcggaa cgggaatcgc gggcgcaagg gcgaccggag gcggaggaag
     1381 aatgggcccg cgaaacttcc accagatgtc atatgatcca ggatcacgcg gaagatgagg
     1441 atactgcctt ccgccgtcgc ctccggggtg aggaacgcgt ggcccgtcgg acgtgctggc
     1501 ttgcctgtgg tggcgccacc agggatagcg tgaggcgtgg ctcccatctt ctttgtgctt
     1561 gcgccaccag gggtaacgcg tcactcggct ttcgaccgct tggggcctgc gccgccaggg
     1621 aaagcgtgtc ttgcggctct cagtggacgg gcttcttgaa tgaaacccgt tcttacggaa
     1681 gtaagagttg gaccttccga aagagccggg catacgctgt gcatggtagc gattgaagcc
     1741 gcctccgcta tgtcctccgt taaacgcaac ggcggcgggc attgctgcac gttgttcgtg
     1801 aagtcgatag gcgggatatg ccaacgcttg accgacatcg aaaccaaata atgcgatcaa
     1861 tagaactgcc gcagcgcggc cagacgccca ttctgacatg gcgacgaatg ccctttgaac
     1921 gacccgtgtt acttctcggg caataccagc ttcacagctg gcgaggcccg caatttcctg
     1981 taagcgattt cctgcgcttc gactgtatcc cccggcacgg cgaggtgata gagcgcatcg
     2041 gcactcggcc cttcgacaat gcttgcgcca gcgtcgtcga ggagcgtgtt gatctccgcc
     2101 atcgtggctg tctgtgagaa tgagacgata aacgcacgct tcgccgctgc gttgctcgct
     2161 gggccagaag ccgtg
//
LOCUS       DATMGX010000387         2423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_240925, whole genome shotgun sequence.
ACCESSION   DATMGX010000387 DATMGX010000000
VERSION     DATMGX010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240925"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            671..802
                     /locus_tag="VNR65_06275"
     CDS             671..802
                     /locus_tag="VNR65_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18316.1"
                     /translation="MHLAIEVAADATGTALVIPGAGLFTYYDGRQAAAYGIADQNES"
     gene            840..1367
                     /locus_tag="VNR65_06280"
     CDS             840..1367
                     /locus_tag="VNR65_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWJ18317.1"
                     /translation="MPPSKSFADLNADQARAAQHGAGGGPLLIIAGAGSGKTQTLAHR
                     VAYLISEDAVPGRILLITFSRRAAQEMERRVERILTGRGMSQGLTSIGWSGTFHAVGA
                     RLLRDFAHSIGLNPAFTIHDREDSADLLNLVRHQMGFFEQEQRFPLKWVSPSRPRTPV
                     ISWEHGWRQAPGPRG"
     gene            1333..2310
                     /locus_tag="VNR65_06285"
     CDS             1333..2310
                     /locus_tag="VNR65_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HWJ18318.1"
                     /translation="MDGAKHPVPGVDYPRTFQEFDEWFRSDYSCREYIRHLRWPDGFI
                     CPNCGMAGEAWLMSRGRFRCRSCQRETSLTAGTIFADTRKPLRMWFLAMWFVTSQKNG
                     VSALGLQRVLGLGSYETAWTWLHKLRRAMVRPSRDSLTGAVEVDEIYIGGPEEGMRGR
                     QTETKAIVAVAAEKSGRGIGRIRLRRVMDVSADSLLPFVKEAVTPGSLVHTDGWSSYS
                     SLAKAGYKHQVNVINNGTELAHEVLPRVHMAASLLKRWLIGTHQGGVQHQHLDYYLDE
                     FTFRFNRRHSKARGLLFHRLAQQAAAVGPAPYHVIVTDTQTAASMAQRA"
BASE COUNT          501 a          694 c          709 g          519 t
ORIGIN      
        1 catcccgcaa aagctgcgcg catttcttga ttttgttctg ccccgcctga aagcgaagct
       61 ggtgttcgat ccacaataaa ttccagcgtt gaagatccga tttccccttg gcattgcttc
      121 tcgcgaagct ccatcgcctt agacctgccc agaagccaat cgatcgaaac gtcaagccgg
      181 tctgctatct gagccagccg gtaaatgtca agcgcgcgga ggcccgcgtg ttcaaggtcg
      241 acagtgcgcc ggccgccaag cccaatgctt gaacagagct ggtcatatgt gatgccgcga
      301 aggcggcaag cgattgcgag ccgctcccgg aatatgaaga aattcgtttc catacttcgg
      361 atatgcgtca gccgaggatg cgccgcaaga tgctacagca gcgccgcaaa cgcacttacg
      421 gcagcacttc gtagatctgc ggctcgctcg cgtgcttctc tctgctcgcg gaaatcgtga
      481 aataagcgca aatctcgctc ggatttgttc gggaaaaccg gtttaaccga ttgatttttc
      541 ttggctgtaa gaacagccat tcgggagcta agctattgtt tttatgcaaa aacgcagggc
      601 ctgaaaatcc gtgttcccgt ggtacgagtc cgtccactga agcgatgcgc acagcgcaac
      661 ggctgctcgc atgcatttag ccattgaggt ggctgcggat gcgacaggga cagcgctggt
      721 gattccgggt gctgggcttt tcacgtacta cgacggccgg caggctgcgg cgtatggcat
      781 agcggaccag aacgaatcgt gatcattttg atctttggga tcaaactcag ctcagagcca
      841 tgccgccatc taaaagtttt gcagacctca acgcggatca ggctcgcgcc gcgcaacacg
      901 gcgcgggcgg tgggcccttg ctgattattg cgggggcggg ttctggcaag acccagacgc
      961 ttgcgcatcg ggtggcttat ctcatcagtg aggacgccgt tcctggccgc atcctgctta
     1021 ttaccttttc ccggcgcgct gcgcaggaaa tggagcgcag ggtcgagcgc atcctcaccg
     1081 gccgcggcat gagccagggg ctcacttcta tcggctggtc cggtaccttt cacgccgtcg
     1141 gcgcgcggct tctgcgcgac ttcgcacatt ccatcggcct caatcctgct tttaccattc
     1201 acgaccggga ggattccgcc gatctcctga acctcgtccg ccatcaaatg gggttcttcg
     1261 aacaggagca acggtttcca ctgaaatggg tatccccttc acgccctcga accccagtga
     1321 tatcgtggga gcatggatgg cgccaagcac ccggtcccag gggttgatta tcctcggacg
     1381 tttcaagagt tcgacgaatg gttccgcagc gattacagct gtcgggagta tatccgccac
     1441 ttgcggtggc cggatggttt catctgcccg aattgtggaa tggcgggcga ggcgtggtta
     1501 atgtctcggg gccgatttcg atgccgcagc tgtcagcgag aaacgtcact gacggccggc
     1561 acgatattcg cagacacccg gaagccgctt cgcatgtggt ttctcgccat gtggttcgtg
     1621 acgagccaaa agaacggcgt cagcgcgtta ggcttgcagc gggtgctcgg attgggaagc
     1681 tacgaaacag cctggacgtg gctccataag ttgcgccggg cgatggttcg acccagccgg
     1741 gatagtctga ccggagctgt cgaggtggac gaaatctata tcggcggccc ggaagaaggg
     1801 atgagaggcc gccaaacaga aaccaaggcc atcgtcgcgg tagcagccga gaaaagtgga
     1861 cggggcatcg gccgcattcg tcttcggcgc gtcatggatg tgtccgccga tagcctcctt
     1921 ccgttcgtaa aggaagctgt gacgccaggc tcccttgttc atacagacgg atggagcagc
     1981 tattccagtc tggcgaaggc cgggtataag catcaggtca atgtcatcaa taatggtacc
     2041 gaattggcgc atgaggtttt accgcgtgtc catatggccg cttcgctcct caagcgatgg
     2101 ctgatcggta cccaccaggg tggcgttcag caccagcacc tcgactacta ccttgatgag
     2161 ttcacatttc gattcaatcg ccggcactca aaggccagag ggctgctttt tcacaggctg
     2221 gcacaacagg ccgccgctgt tgggcccgcc ccatatcacg tgatcgtcac cgacactcaa
     2281 accgctgcct cgatggcgca gcgggcgtga aggggatacc cattttctcg aataacaaca
     2341 gccctttcaa gaagccgggc agctatcatg cggtttccga ggtttgggga taaggccctg
     2401 gccccgctga cgacccgaac tgc
//
LOCUS       DATMGX010000388         2074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_386145, whole genome shotgun sequence.
ACCESSION   DATMGX010000388 DATMGX010000000
VERSION     DATMGX010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386145"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VNR65_06290"
     CDS             <1..531
                     /locus_tag="VNR65_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWJ18319.1"
                     /translation="DLSGRGVGMDVVRTNIEKMGGQVTVTSVVGDGATVRLSLPLSMA
                     VTRVMMVEVGNSLVGIPMDVIVETVRLSSKQIYAIKQSETFVLRDTIIPLVRMDRLLG
                     VSGGTGNQNELGMAVLVVRVRGALVGLSVDQFRESIDVILKPFGGLLSGLTGYAGTAL
                     LGDGRVLLVLNLKEIL"
     gene            579..773
                     /locus_tag="VNR65_06295"
     CDS             579..773
                     /locus_tag="VNR65_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18320.1"
                     /translation="MVEDALPLLEFLRTHEGVTIDMGDCTYAHTAVLQALAAATKELK
                     LPASPEMARWVGAVMGATCV"
     gene            781..1200
                     /locus_tag="VNR65_06300"
     CDS             781..1200
                     /locus_tag="VNR65_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ18321.1"
                     /translation="MRYAVPDLVNMGALRMAKTIMIVDDSQTMLMSMEGMLSKAGLGV
                     SKANNAEEALVILKGGSKPDMIITDLNMAAMNGIQLIREVRRLPGYQFMPIVMLTTES
                     QQEKRDEAKSAGATGWMVKPVQPQSMLQIVKQLVPGA"
     gene            1324..>2074
                     /locus_tag="VNR65_06305"
     CDS             1324..>2074
                     /locus_tag="VNR65_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWJ18322.1"
                     /translation="MVAAFFITALAMVAARRLGTASTTVRREAPTAGDRKPLLISAPI
                     HQRHASVDPLVLKRLGEEISACNPIVFTLCDHVQDVVANTEDVAMAIMTQLNRVDQTV
                     TELLRYLQVSSHDRILPIIEQTEQRLRENSQILTGFLTNRMAAMDSSRSHLSCIADLA
                     GRLDTIVQSIRKVARQTNMLALNATIEAARAGVPGRGFAVVASEVKALSQQTDQAAKD
                     ISEGLQTLKAAIAESIETLVVRQGKDERKDLD"
BASE COUNT          456 a          564 c          618 g          436 t
ORIGIN      
        1 gatctctcgg gccgcggcgt gggcatggat gtcgtgcgca ccaatatcga aaaaatgggg
       61 ggccaggtca ccgtcacgtc ggtcgtcggc gacggagcga cggtgcgcct gtcactgccg
      121 ctatccatgg cggtcacccg cgtcatgatg gtcgaggtcg ggaacagcct cgttggcatt
      181 cccatggacg tcatcgtgga aaccgttcgt ctttcgtcca agcagatcta tgcgatcaag
      241 caatccgaaa ccttcgttct gcgcgacacg atcatccccc ttgtgcggat ggaccgtctt
      301 ctcggggtgt ccggcggcac cggaaatcag aacgaactcg gcatggcggt tcttgtcgtg
      361 cgcgtgaggg gcgctctggt ggggctgtcc gtggatcaat tccgggaaag catcgacgtc
      421 attctgaagc ccttcggtgg gctcctgtcc ggcctgaccg gctatgcggg gacggcgctt
      481 ttgggcgacg gccgggtttt gctggttctg aacttgaagg aaatactttg atgccaatcc
      541 gctttgaagc atcaactgtt tattgccaag gcgagtgtat ggttgaggac gctctacctc
      601 tgctggaatt tcttcgcaca cacgaaggtg tcacgatcga catgggagat tgcacctacg
      661 cccacacggc ggtcctgcaa gctctggcgg cggcgacgaa agaactcaaa ctcccagcat
      721 cgcccgaaat ggcgcggtgg gtcggtgctg ttatgggggc aacctgcgta tagcgcgtcg
      781 atgcgttatg cagtccctga tttggtaaat atgggagcgt tgcgtatggc caagacaatt
      841 atgattgtgg acgactccca gaccatgctc atgagcatgg aaggcatgtt gtcaaaagct
      901 ggcctcggtg tttccaaagc caacaatgcc gaagaggctc ttgtcattct gaagggcggc
      961 agtaagccgg atatgattat tacagacctg aacatggcag cgatgaacgg catccagttg
     1021 atccgcgaag tccgcaggct tcctggatac cagttcatgc cgatcgtcat gctgacgacg
     1081 gaatcccagc aggaaaagcg ggatgaagcc aagtccgcgg gcgccacggg gtggatggtt
     1141 aagccggttc agcctcaatc catgttgcag attgtcaaac aactcgtccc tggagcgtga
     1201 gcaatgcggc tgcggtacga ttacgctgcc atcgtagcgc tcgcttccgc agcggcggtt
     1261 ttgaccgcgc tgagcgacta tgctctcagc atggaaaaaa gcctggacgc cctaggcctt
     1321 cgcatggtgg cggccttctt catcaccgcc ctcgctatgg tggcagcacg ccggttgggc
     1381 accgcctcga caaccgtaag gcgcgaggcg cccaccgcgg gtgatcgcaa acccctgctt
     1441 atatcggctc ccatccatca acgtcatgct tctgttgatc cgctggtcct gaaacggctc
     1501 ggagaagaga tatcagcctg caatccgatt gtttttacgt tgtgcgacca tgtccaggac
     1561 gtcgtagcga atacggaaga tgtcgctatg gcgatcatga cccagcttaa cagggtggat
     1621 caaacagtaa cggagctgtt aagatatttg caagtcagct cgcatgatcg gattcttccg
     1681 atcatagaac agacagagca gcgacttcgt gaaaactccc aaatcctgac cggttttcta
     1741 accaaccgaa tggccgcgat ggacagcagc cgtagccacc tttcctgcat tgccgattta
     1801 gcagggcggc tcgatacgat cgttcagagc atccgaaaag tagcgcggca gaccaacatg
     1861 cttgcgctga acgccacgat tgaagcggca agggcaggag tgccggggcg cggtttcgcc
     1921 gtcgttgctt ccgaagtaaa agcgctgtca cagcaaacag atcaggcagc gaaggacatc
     1981 agcgaaggtc ttcaaacatt gaaagccgcc atcgctgaaa gcatagagac cctggtcgtg
     2041 cggcagggta aggacgaacg caaggatctg gacg
//
LOCUS       DATMGX010000389         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_301065, whole genome shotgun sequence.
ACCESSION   DATMGX010000389 DATMGX010000000
VERSION     DATMGX010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301065"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..975)
                     /locus_tag="VNR65_06310"
     CDS             complement(94..975)
                     /locus_tag="VNR65_06310"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ18323.1"
                     /translation="MKIAFLGLGNMGGHMARHLLRAGHDITVWNRTLSKADELRAKGA
                     KVGKSAGEAVKDAEAVVTMLADDHAVESAVLHSEGVLDHLPRGAVHISMSTISVALSE
                     KLAEEHGKHGQQYIAAPVFGRPEAAEAGKLFVVAAGDKSAVERCKPLLELLGQRVFVI
                     GDKPEMANVVKLSGNFLIASVIESLGEAIALTRKYGIDPHDYVEFLTNSLFAAPIYKT
                     YGGIIAEEKYQPAGFKLRLGLKDVRLALAAAEAVDAPLPFASVVRDHMLTAIGRGMED
                     MDWSSTAKLAAENAGLK"
     gene            1132..>2318
                     /locus_tag="VNR65_06315"
     CDS             1132..>2318
                     /locus_tag="VNR65_06315"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HWJ18324.1"
                     /translation="MATKQERLSTHEERRKFGQSRRDEVSRVSQGDWTPKRRDFGVVD
                     LLRKAQRGRLSKLLPIKFARMATSPFGFFRGAVPVMAADLSKLPNTGIHAQICGDAHV
                     RNLGAYAGPDGRLIFDINDFDETIRGPWEWDVKRMAASLMLAGREADNTEKECKEAVL
                     AFARSYREAMRHFCEMAMLEIARYQVFRHLNISPVLSVLRKAERSTPMHNLDRLTVRH
                     DGTYRFREQKPWQFHLQRKTVAEIVAGLRNYVGTLLPERQHWFAQYHVEDVAFRVVGT
                     GSVGVRDYIVLMFGRTTNDPLFIQIKEEDASAYAPYSPQSRILINQGQRAAQGQRAMQ
                     VQSDIFLGWTAIAGRDYLVRQLRDHKAGIENEDLEGNGLVQYGRVCGELLSKGHARSG
                     DACA"
BASE COUNT          468 a          709 c          642 g          499 t
ORIGIN      
        1 tgcgcgcgat tcggttgagt tcgggtcacg attgcctctg tcaagatact acaacattaa
       61 agcaatccgc tcatcccaaa cccgtttgtg cagttacttc agcccggcat tttccgctgc
      121 cagttttgcc gtcgaactcc aatccatgtc ctccatgcca cggccgatgg cggtcagcat
      181 gtggtcgcgc actacactgg cgaagggcag aggcgcatct acagcctcag ccgcggccag
      241 ggcaagccgc acatctttca gtcccaggcg cagtttgaag cccgcaggct gatatttctc
      301 ctcggcaata atgccgccat acgtcttgta tatcggagcg gcgaacagag aattggtcaa
      361 gaactccaca tagtcatgcg gatcgatgcc gtacttccgc gtgagcgcaa tcgcttctcc
      421 cagcgactcg atcaccgaag cgatgaggaa gttgccgctc agctttacca cgtttgccat
      481 ttccggctta tcgccgatta cgaacacacg ttgccctaat aactccaaca gcggcttgca
      541 gcgttctacg gccgatttgt ctcccgccgc caccacaaac agctttcctg cttctgccgc
      601 ttctgggcgt ccaaaaaccg gggcagcaat gtattgctgc ccgtgcttgc catgttcttc
      661 ggccagcttc tccgacagtg cgacgctaat ggtgctcatc gagatgtgca ccgcgcctct
      721 tggcagatgg tccagcactc cttccgaatg caatacagcc gattcaacgg cgtgatcgtc
      781 ggccagcatg gtgacgacgg cttccgcgtc tttcacggcc tcgcctgccg atttgcctac
      841 cttggcgccc ttcgcgcgta gctcgtccgc tttcgatagg gttcggttcc ataccgttat
      901 gtcatgtccg gcacgaagca ggtgacgggc catgtggcct cccatattgc ctagtcctag
      961 aaatgcgatc ttcattgcgg gcctctttca tcttgactta caggttgcac agatgcaggg
     1021 aaggcggctt tggctgcgca gaaaagtcac tgtcgtagcg cgccaacttg gtggcgccgc
     1081 tcttttaaat ccctgcccag gccgcaattt cgggtatgat gcatcagttc catggccaca
     1141 aagcaggaaa gattatccac ccacgaagaa cggcgaaagt tcggccagtc tcgccgcgac
     1201 gaagtgagcc gcgtcagcca aggcgactgg actcccaagc gccgcgactt tggcgttgtg
     1261 gatttgctac ggaaagcgca gcgcggacgc ctctccaagc tcttgcccat caagttcgca
     1321 cgcatggcaa cttcgccctt cgggttcttt cgcggggccg ttccggtgat ggccgcggac
     1381 ctttccaagc ttccgaacac agggattcac gcgcagattt gcggcgacgc gcacgttcgc
     1441 aacctgggag cttatgctgg cccggacgga cggctcattt tcgacatcaa cgacttcgac
     1501 gaaaccatcc gtggaccttg ggaatgggac gtaaagcgca tggctgcgag cctgatgctg
     1561 gcgggccgcg aagccgacaa caccgagaag gagtgcaagg aggctgtgct cgctttcgcg
     1621 cgcagctatc gtgaggcgat gcgccacttc tgtgagatgg caatgcttga aatcgctcgc
     1681 tatcaggttt tccgccacct gaacatctcg ccggtgctct ccgtgttgcg taaggctgag
     1741 cggtctacgc ccatgcacaa tctcgatcgc ttgaccgtgc gacacgacgg tacgtatcgc
     1801 ttccgcgaac agaagccgtg gcaatttcac ctgcagcgca aaacggtagc ggagatcgtt
     1861 gcagggttgc gcaactatgt cggaacgctc ttgcccgaaa gacaacactg gttcgcgcag
     1921 taccacgtcg aggatgtcgc ttttcgagta gtcggaaccg gcagcgtcgg tgtacgcgac
     1981 tacattgtcc tcatgtttgg ccgtacaacc aacgatccgc tgttcatcca gatcaaagaa
     2041 gaagacgcgt ctgcgtatgc gccctactct ccccagagcc gcattctcat caaccaaggg
     2101 cagcgtgcgg cccaaggaca gcgcgccatg caggtgcaat ctgacatttt cctgggatgg
     2161 acggccatcg ccgggcgcga ctacctggtc cgccagttgc gcgaccacaa ggccggcatt
     2221 gagaacgaag atctcgaagg caacggactg gtacagtacg ggcgcgtttg tggcgagttg
     2281 ctttccaagg ggcacgctcg ctcaggcgac gcatgcgc
//
LOCUS       DATMGX010000390         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_294886, whole genome shotgun sequence.
ACCESSION   DATMGX010000390 DATMGX010000000
VERSION     DATMGX010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294886"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2421)
                     /locus_tag="VNR65_06320"
     CDS             complement(<1..2421)
                     /locus_tag="VNR65_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HWJ18325.1"
                     /translation="MGSQSHQYGSCDNAPTKVDVQLSALVQTASPADSLPARVLAKSR
                     EVAGRTAVYTVGMDVGSTTVKAVIVNAETDQIIWQDYQRHETKQPEKMLEFLKRMEEE
                     VGIAPHNCRIFITGSGGGALANLIGAKFVQEVTAVSLAVEKLHPEVNSVIELGGQDAK
                     IIVFKEDENTGRKKKIPSMNDKCAGGTGAVIDKINAKLKIPAEVLCNQGYTGIKLHPV
                     AGKCGVFAETDINGLQKQGIPPDQLMASLFDAIVLQNLTVLTRGHTLRPHVLLLGGPN
                     TFIRGMKEAWQQNIPRTWEERKVELPEGAKAEDLIKVPDNAQYFAALGAVEFGKDEES
                     EVGIYMGTECLEDYITVGRQAEKAKSGAAGLSISEEELAGFKQKYTIPRFIGAHFGVG
                     ETVRGFVGLDGGSTSTKAVLLSETGDVLCKAYQLSDGNPIQDTINMFMALRHQVESQG
                     ATLEVLGVGTTGYAKDVLKDVLKADVALVETVAHTESAMKFYKNPHVIVDVGGQDIKL
                     IVLKDGRVKDFKLNTQCSAGNGYFLQGTAESLGYKVEEYADVAFSANTMPIFGYGCAV
                     FMQSDIVNFQRQGWRGEEILAGLANVLPKNIFLYVAGIPNLAKLGSRFILQGGTQKNL
                     AAVKSEVDFIRSHFHGVDHEADVIVHEHCGESGAIGAGVEAIRLYKKGLRTTFIGLDP
                     VQNIGYRTTRNEDTRCYFCKNNCLRTFIDVTVGEVEVSSPLPVFKSKVPLQAGERRLI
                     ISTCEKGQVEDVGAMRSIKAGLDAVKNANPNLVDIAAREVWRSRNPQKVADPVPTRAW
                     TSAAKQRIA"
BASE COUNT          492 a          794 c          769 g          582 t
ORIGIN      
        1 cgcgatgcgc tgcttggcag ccgacgtcca ggcgcgggtc ggaacggggt cggcgacttt
       61 ctgcggattg cgcgagcgcc acacctcgcg ggcggcgatg tccacgagat tcgggttggc
      121 gttcttgacc gcgtctaagc ctgccttgat actgcgcatt gccccgacgt cctcgacctg
      181 ccctttttcg cacgttgaga tgatcaggcg ccgttcgccc gcctgcaaag gcaccttcga
      241 cttaaagacc ggtagcggcg acgagacctc aacctcgccc acggtcacat caatgaacgt
      301 gcgcaggcag ttgttcttgc agaagtagca ccgcgtgtct tcgttgcggg tggtgcggta
      361 gccgatgttc tgaacgggat cgaggccgat gaaggtagtg cgtaggcctt tcttgtagag
      421 acgaatggcc tccacgcccg cgccaatcgc ccccgactcg ccgcagtgct cgtgcacgat
      481 gacgtcggct tcatggtcca cgccgtggaa gtgagaacgg ataaagtcga cctcggactt
      541 caccgcagcc aggtttttct gcgtgcctcc ctgcaggatg aaacgcgacc cgagttttgc
      601 caggttggga atgccggcga catagaggaa aatgttcttg ggcagaacgt tcgccagccc
      661 ggcgagtatc tcttcgccgc gccaaccctg ccgctggaag ttgacaatgt cggactgcat
      721 gaacaccgcg cagccgtagc cgaagatcgg catggtgttg gcggagaatg cgacatcggc
      781 gtactcctcc accttgtaac cgagcgactc ggcggtacct tgcagaaagt agccgtttcc
      841 ggccgagcac tgggtgttga gtttgaagtc cttgacgcga ccgtccttca acacgatcag
      901 cttaatgtcc tggccgccga catccacgat gacgtgggga ttcttgtaga acttcatggc
      961 cgattcagtg tgcgccacag tttccaccag cgcgacatcg gccttgagca cgtctttcaa
     1021 gacgtccttg gcgtatcccg tcgtgccgac accgagcact tccagggttg ctccctgtga
     1081 ctccacctgg tggcggagag ccatgaacat gttaatagtg tcctggatgg gattcccgtc
     1141 agacagttgg taggccttgc acagcacatc gcccgtttcc gagagcaata cagctttggt
     1201 cgaggtcgaa ccgccgtcaa ggccgacgaa tccgcgcacg gtctcgccga caccgaagtg
     1261 ggcgccaata aatctcggga tcgtgtactt ctgtttgaag ccggcgagtt cttcctcgga
     1321 gatgctcaga ccagcggccc ccgacttcgc cttctcagct tgccggccga cggtgatgta
     1381 gtcctcgagg cactcggtgc ccatgtagat accgacctcg gactcttcgt ccttgccaaa
     1441 ctccacggcg cccagcgcgg cgaagtattg cgcgttgtcc ggaactttga tcaggtcttc
     1501 cgctttggcg ccctcaggaa gctcgacttt gcgctcctcc caggtgcggg gaatgttctg
     1561 ctgccacgct tctttcatgc cgcgaatgaa cgtattggga cctcccagta gcagcacgtg
     1621 gggacgcagc gtgtgcccgc gggtcaagac tgtgaggttc tgcagaacga tggcgtcgaa
     1681 cagagaagcc atcaactgat cgggcgggat gccttgcttc tgtaggccgt tgatgtcggt
     1741 ttcggcgaag acgccgcact tgcccgcgac cggatgcaac ttgatgccgg tgtagccttg
     1801 gttgcagagc acttcggccg gaatcttgag cttcgcattg atcttgtcga tgacggcgcc
     1861 ggttcctccc gcgcacttgt cgttcatcga agggattttc ttcttgcggc cggtattctc
     1921 gtcctccttg aagacgatga tcttggcatc ctgcccgccc agttcaatga cagaattcac
     1981 ctcggggtgc agcttttcga ctgcgagcga gactgcggtg acttcctgca cgaacttcgc
     2041 gccgatcagg ttggccagcg cgcctccacc cgagccggtg atgaagatac ggcagttgtg
     2101 cggagcaatg ccgacttcct cctccatgcg cttgaggaat tccagcattt tctcgggctg
     2161 cttggtctcg tgccgctggt agtcctgcca gatgatctgg tcagtttcgg cgttgacgat
     2221 gactgccttc accgtcgtcg agccaacatc catgccgacc gtgtagacgg ccgtgcgacc
     2281 agcgacctcg cgcgacttgg ccagaacccg cgccggaagc gaatcagcgg gcgacgccgt
     2341 ctgcaccagg gccgaaagtt gaacgtcaac cttggttggg gcgttgtcgc aggatccgta
     2401 ctggtgagac tgtgaaccca ctaatcctcc cgaaaaaccg aaccggcgat gaaatacatc
     2461 agtcgccgaa gtttccgcta accccctgaa acccgtgcct ggcctgctga aacagccagc
     2521 gaatcacacg ctgatcgtta tagcaaaaag ccaggatgtg gttcagggtg gaaaagcagc
     2581 tcttctggca ctccttcttc atgtctttca actgcttcgc ttcgaatttg tggttct
//
LOCUS       DATMGX010000391         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_199609, whole genome shotgun sequence.
ACCESSION   DATMGX010000391 DATMGX010000000
VERSION     DATMGX010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199609"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1190
                     /locus_tag="VNR65_06325"
     CDS             21..1190
                     /locus_tag="VNR65_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HWJ18326.1"
                     /translation="MSGAPKGRPPVAFSLDDPRLIDAPPPPPKEVEAKPAPKPAPSAA
                     PSSTARAQTRPRVESPKVDAPKAEAAGRAEAPAGEGTSAPSPGEISNVPEIVRVGRSW
                     GSIFLSAVTGLLFLAFLVWVQDTFLTLLGRKDWLGWAATLLLGTAVFAFVMILSREIA
                     GLYRLAAITLVRQRADAALAAENASAARSASGEIRAMLEERRELADGVARLKRHQGDI
                     LDAKQVLTLTERELLFPLDKDARVTVGTSGRRVSMVTALSPAAVFSVGFVAYENFRML
                     RTLAGIYGGRPGFISVLRLMRLIAAHLAVTGGIAFTDDILGQFLGHGMTSRLSKRLGE
                     GLINGGFTIRIGIAAIEVLRPLPYIEARQPRLREFVSEFLKVGQKPDMNNGKERG"
     gene            1543..>2498
                     /locus_tag="VNR65_06330"
     CDS             1543..>2498
                     /locus_tag="VNR65_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HWJ18327.1"
                     /translation="MLSRLKIAIRIDLLLVIAAVGMVICSGIGLWTLRKQMLEDRRVQ
                     LRNVMELVIQHALGDMNEKGGPQSEAGRNAFFDMLGKIRYGDNLADYFFSFDYNGVLL
                     LHPNREMVGSNYFNRDFNGFYATRKFIEIALSPSGSGFVEYNYGMHVRKLAHIRNVPE
                     LHALVGLGIRVEDVDALFMARLRDEAWLFAVTLPLISLCCFAVSRSITGPLSNILCKI
                     TRLAKGDLDIPPAHEVDRSELGNVANAIEILRCNAIEQRELQEKVHQQNEQLLEQMQK
                     AEQAVTAKSEFLANMSHELRTPMHAILGYADISLTDINEGTP"
BASE COUNT          510 a          709 c          730 g          549 t
ORIGIN      
        1 ctgatcggtg acaaactatc atgagtggcg cacccaaagg caggccgccg gtggccttta
       61 gtctcgacga ccctcgcttg atcgatgctc cgcctccgcc gcctaaggaa gtggaggcca
      121 agccagcgcc gaagcccgcg ccgagcgctg ccccaagttc gacggccaga gcccaaacgc
      181 gcccaagggt cgagtcccct aaggttgatg ctcctaaggc ggaggcagca gggagggccg
      241 aggctccggc gggagagggc acgtctgctc cgtcgcccgg cgagatttca aatgtgcccg
      301 agatcgtccg cgtcgggcgt agctggggca gcattttctt gagcgccgtc accggcctcc
      361 tcttcctcgc ctttctagtc tgggtccagg acacgttcct gacattgctg ggccggaagg
      421 attggctcgg ctgggcggcg acactgctcc tgggaaccgc ggttttcgcc ttcgtcatga
      481 tcctttcgcg cgaaatcgcg gggctttatc gcctggcagc gatcaccttg gttcggcagc
      541 gcgcggacgc ggccttggcg gccgaaaacg catccgcagc tcgcagcgcg tcgggagaga
      601 tccgcgcaat gcttgaggag cggcgcgaat tggcagatgg ggttgctcgc ctcaagcggc
      661 atcaaggcga cattctggat gcgaaacagg tcctgacgtt gaccgagcgc gagctcttgt
      721 tcccgttgga taaggatgct cgcgtaaccg tcgggacttc cggccgccgg gtgtcaatgg
      781 tgaccgcttt gagcccggcc gcggtcttct cggtcgggtt cgtggcctac gagaatttca
      841 ggatgctccg cactcttgct ggcatctacg gaggaaggcc cggcttcatt tccgttctgc
      901 gcctcatgcg cctcatcgca gcgcatcttg cggtgacggg cggcatcgcg tttacggacg
      961 acattctcgg ccaattcctt ggccatggca tgacatcgcg tctttccaag cggcttggcg
     1021 aggggctcat caacggcggt ttcacgatcc gcatcggcat cgccgccatc gaggttttgc
     1081 gtccgctgcc ctatatcgag gcgcggcagc cgcgcctgcg cgagttcgtt tccgagttcc
     1141 tgaaggtcgg gcaaaagccg gacatgaaca acggcaaaga gcgcggttga tccgagcctt
     1201 cagagcgttc ggctcatagc atcaattgag cttttcccaa cctgggcatc atcttgtgag
     1261 ggtgtacaaa gggttaaaat tggttttgtt tggtaagatg accataagta aatatacagg
     1321 tagtttttcc caccatgaac gacggccatc gcaggcgatt ttgttttcgg catctcgccg
     1381 actgtgttcg gttggcgtgt ggcgccgctt gaccccaaaa gccagcacgc tggagagcct
     1441 cctgagaccg gagggcgtgg ccgtgcgcat tattggcaat caagcgttca ctaagaccgt
     1501 gatataggct agagcaccac aatcttaagt atgggtggta gcttgttatc tcgacttaag
     1561 attgcaatac gcatcgatct gcttctcgtg attgccgctg tcggaatggt aatttgttcc
     1621 ggcatcgggc tatggactct acgaaagcag atgctggagg accggcgtgt gcagctccgc
     1681 aatgtgatgg agctggttat ccaacacgcc ctcggcgata tgaatgaaaa aggcggccca
     1741 caaagcgagg ctggacgcaa cgcatttttc gacatgctcg gaaagataag atacggcgat
     1801 aacctagctg attatttttt cagttttgat tacaacggcg ttcttctgct gcatcctaac
     1861 cgggaaatgg tcggaagtaa ctatttcaac cgcgatttca atggctttta cgcaacgcga
     1921 aagttcattg aaatcgccct gagcccgtct ggttccggat ttgtcgaata caactacggc
     1981 atgcatgtgc gcaaactcgc gcacatccgc aacgttcccg agcttcacgc ccttgttggc
     2041 ctcggcatac gtgtcgaaga tgtcgacgct ctgtttatgg cccggctccg cgacgaggca
     2101 tggttgttcg ccgttacgct gccgcttatc tcgttgtgct gtttcgcagt cagccgttcc
     2161 attacaggtc cgctgtcgaa cattctatgc aaaatcacgc ggcttgcgaa aggcgattta
     2221 gacatccctc cggcgcatga ggtggatcgg tccgagctcg gcaatgtcgc caatgcaatc
     2281 gagattctgc gctgcaacgc gatcgagcag cgcgagcttc aggaaaaggt gcaccagcaa
     2341 aacgaacagc tgctcgagca aatgcagaag gcagagcagg cagtcacagc aaaatccgag
     2401 tttctggcga acatgtctca tgaactcaga acgccaatgc acgccatact tggttacgca
     2461 gacatcagcc ttaccgacat caacgaagga accccgca
//
LOCUS       DATMGX010000392         2067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_71984, whole genome shotgun sequence.
ACCESSION   DATMGX010000392 DATMGX010000000
VERSION     DATMGX010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71984"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..>2067
                     /locus_tag="VNR65_06335"
     CDS             190..>2067
                     /locus_tag="VNR65_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWJ18328.1"
                     /translation="MNFEKLTDRSKGFFQSAQSLALREGHQRLTPEHLLKVLLDDSEG
                     LAAGLITRAGGRPEDALARVEQALAKMPKVSGAGAGQVYISPQLAKVLDQAEKIAEKA
                     GDSYVTVERLLLAMAMEKDADTSKILAEAGVTPTGLNAAINELRKGRTADSASAEQAY
                     DALKRYARDLTDAAAKGQLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLALRIVNGDVPESLRDKKLLALDMGALIAGAKYRGEFEERLKSVLQEITSAEGGVV
                     LFIDELHTLVGAGKAEGAMDASNLLKPALARGELHCVGATTLDEYRKYIEKDAALARR
                     FQPVFVSEPTVEDTISILRGLKEKYELHHGVRITDSALVAAATLSNRYITDRFLPDKA
                     IDLMDEAGSRLRMQVDSKPEELDELDRRIIQLKIEREALKKESDQASKDRLARLQKEI
                     ADLEERSSSLARRWREEKDKLASAQKIKEELDAARNELEQAQRKGNLGRAGELSYGVI
                     PDLEKKLKAMENAADSNRGMVEEAVTPDHIAGVVSRWTGIPVEKMLQGEKDKLLRMED
                     QLGKRVIGQAEAVHAVSTAVRRARAGLQDPHRPIGSFMFLGPTGVGKTELARTLAAFL
                     FD"
BASE COUNT          453 a          614 c          643 g          357 t
ORIGIN      
        1 cgtgagtatt gcttgcgcct tcgccaggcc actcaatccc ttcacagggt aatgcacgag
       61 cttggcaatt ttcagcggcg gaggcatgtc cggtgacctt ttcttgtctg ccaatggtgc
      121 catatatcaa cacatccatg ctaactctcc cacgaattag cggcgaaggc cgcagccatt
      181 gaggcgctaa tgaatttcga gaaactgacc gatcggtcca agggcttctt tcaatccgcc
      241 cagtcgcttg cgcttcgtga aggccatcag cgcctgacgc ctgagcacct cctcaaagtt
      301 ttgctggatg acagcgaagg tttggccgcg gggctgatca cgcgcgcagg gggccgccca
      361 gaagatgcgc tcgcccgcgt cgaacaggcg ctggcgaaga tgccgaaggt gtcgggcgcc
      421 ggcgcggggc aggtctacat ttcgccgcaa ctcgcaaaag ttctcgacca ggctgagaag
      481 atcgcggaaa aggccggaga cagctacgtc actgttgaga ggctgctcct cgcgatggcg
      541 atggagaagg atgccgatac ttcaaaaatt ctggcggagg ctggtgtcac gcccacgggt
      601 ctgaacgccg ccatcaacga actgcggaag ggccgcacgg cggacagcgc ttctgccgag
      661 caggcttacg acgcgctaaa gcgttacgcc cgagatctga ccgatgccgc tgccaagggc
      721 caactcgatc cggtgatagg ccgggatgag gagattcgcc gcaccatcca ggttctctcc
      781 cggcgcacga aaaataaccc tgtgctgatc ggcgagccgg gggtcggaaa aaccgccatc
      841 gccgaggggc ttgccttgcg aatcgtcaac ggcgacgtgc ctgagagcct gcgagacaag
      901 aagcttcttg cgctggacat gggcgcgctc atcgccgggg caaaatatcg tggcgagttc
      961 gaggagcgac tgaaatccgt gctccaggag atcacttcgg cggaaggcgg cgtcgtcctg
     1021 ttcatcgacg agcttcacac cctcgtcggc gctggcaagg cagaaggggc gatggacgcg
     1081 tccaaccttc tgaagccggc cctggcgcgc ggcgagcttc attgcgtcgg cgcgaccacg
     1141 ctcgacgaat acaggaaata tatcgaaaaa gacgccgcgc tggcccggcg cttccaaccc
     1201 gtcttcgtct cggagccgac cgtcgaggac accatttcga ttctgcgcgg gctcaaagag
     1261 aagtacgaac tccatcacgg cgttcgaatc accgattcgg ctcttgtcgc cgcggccacg
     1321 ctttcgaacc ggtacattac cgaccgcttc cttcccgaca aggcgatcga cctcatggac
     1381 gaggcgggaa gccggctgcg catgcaggtc gattcgaagc cggaggaact cgatgaactc
     1441 gatcgaagga ttatccaact caagatcgag cgcgaggcgc tgaagaaaga gagcgaccaa
     1501 gcctccaagg atcgcctcgc cagacttcaa aaggaaatcg ccgacctgga agaacgctcg
     1561 tcttccctgg ctcggcgttg gagagaggaa aaagacaagc tcgcttcggc gcagaagatc
     1621 aaagaagaac tcgacgcggc gcgaaacgag ctggagcagg cgcaacgcaa aggcaatctc
     1681 ggccgcgcgg gcgaactctc ttacggcgtc atcccggatc tcgaaaagaa gctgaaggcc
     1741 atggaaaacg ccgccgattc gaatcgcggc atggtggagg aggctgtcac gcccgatcac
     1801 atcgcaggcg ttgtatctcg ctggacgggc atcccggtcg aaaagatgct ccaaggtgag
     1861 aaagacaagc tgcttcgcat ggaggaccag cttggcaagc gcgtgatcgg gcaggcggaa
     1921 gccgtgcatg ccgtgtcgac ggctgtaagg cgggcacggg ctgggctgca agacccgcat
     1981 cggcccatcg gctcgttcat gttccttggg ccgaccggcg tcggcaagac agagcttgcc
     2041 cgcaccttgg ccgctttcct gttcgat
//
LOCUS       DATMGX010000393         2490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_175386, whole genome shotgun sequence.
ACCESSION   DATMGX010000393 DATMGX010000000
VERSION     DATMGX010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175386"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2110
                     /locus_tag="VNR65_06340"
     CDS             <1..2110
                     /locus_tag="VNR65_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWJ18329.1"
                     /translation="DPAPGFAAKLGGARSALGVPILKDDVLVGAIVIYRQEVRPFSNR
                     EIELVRSFAAQAAIAIENARLFNETQEALAYQTGSSNVLRVIASSPTDVEPVLKAIVE
                     SAQELCEANDAVALLRDAEDLRFSAHSGPIAITLEKWPISRNWTAGRAFVDQKPVHVH
                     DMLSEEGAEFPDARVMGQGTGSTIHTVLSVPLLRGNESIGAILLRRTEVRPFNDKQIN
                     LLSAFADQAVIAIQNARLFNETREALERQTATADVLKIIAASPTDVQPVFEAIVQTAV
                     RLLGCETSFIQRCDSSSFWSVARCRKEGLLPLLNPRPGPIDPAANFPSRAMLAKKTLY
                     LPDWSEIELPEFERRIQQELGYNCAIYLPLLHKDECLGVLGIAGSRPRMFSEADIALA
                     EAFRDQAVIAIRNTQLFEEVQQRTHELSQSLEDLRTAQDRLVQTEKLASLGQLTAGIA
                     HEIKNPLNFVNNFSALSAELIDELDETLAPAPLDQKMRGDIGELTQMLKSNLEKVVQH
                     GKRADSIVKNMLLHSREGSGEQRPTDINALLDESLNLAYHGARAEKPGFNITLQRDLD
                     PVTGMADVYPQEITRVLLNLISNGFYAATRRAAESDDGFEPTLRAATKNLGDRIEIRI
                     RDNGTGIAPEVKEKIFNPFFTTKPAGEGTGLGLSMSHDIVVKQHGGTIDVDSEPGIFT
                     EFIINLPRATVTQKAAGGTN"
     gene            2107..>2490
                     /locus_tag="VNR65_06345"
     CDS             2107..>2490
                     /locus_tag="VNR65_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ18330.1"
                     /translation="MSTHILVVDDEPDVESMFRQHFRRDLRSGRFIMEFALSAPAALE
                     RVRATADPSLILILSDINMPGMTGLEMLPKVKELRPDVPVIMITAYGDEDTKRRAIAQ
                     GATGLLTKPIDFALLRHEIDARLDQA"
BASE COUNT          571 a          722 c          698 g          499 t
ORIGIN      
        1 cgaccccgca cctggcttcg cagccaagct tggcggtgcg cgatcggcgc tcggcgtacc
       61 gattctcaag gatgatgtac tggttggggc tattgtgatc taccgccaag aggtacgtcc
      121 gttctcaaat cgggagatcg agctggtgcg aagctttgcg gcacaagctg cgatcgcgat
      181 cgagaacgcg cggctgttca acgagacgca ggaggcgctg gcttaccaga ctgggagcag
      241 caatgttctc agggtaatcg cctcttcacc caccgatgtc gagccggtac tcaaggcgat
      301 cgtcgaaagc gcgcaagagc tttgcgaagc taatgatgca gtcgcacttt tgagggatgc
      361 cgaagatctt cgctttagcg cgcattctgg tcccatcgcc ataacgctcg aaaaatggcc
      421 gattagtcgc aattggactg ctggacgggc ctttgtagac caaaagcctg tgcatgttca
      481 cgacatgctt tctgaggaag gcgccgaatt tccggatgcc agagtcatgg gccaaggcac
      541 aggctcaacc atccatactg ttttgtccgt gcccttgctg cgtgggaacg agagtatcgg
      601 cgcgatcttg cttcgccgta ccgaagtgcg tccattcaat gacaagcaga tcaatttgct
      661 gtcggccttc gccgaccaag cggttatcgc gatccagaat gcacgactgt tcaacgaaac
      721 ccgggaggcg ctggagcgac agacagcgac ggccgacgtc ctgaagatca tcgccgcttc
      781 cccgaccgac gttcagccgg tgttcgaggc catcgtccag accgctgtga ggctcctcgg
      841 ctgcgagacg tcctttatcc agcggtgtga cagcagttcc ttctggagtg tggcgaggtg
      901 caggaaggaa ggattactcc cgctgctcaa tccccggcca ggtccaatcg accccgctgc
      961 caactttcca tcgcgcgcga tgctggcaaa gaagaccctt tacctgccag actggtcgga
     1021 aatcgaattg cccgaattcg agcgacgcat ccagcaggaa ctcgggtaca attgcgcgat
     1081 ttatcttccc ttgcttcaca aagacgaatg cctcggtgtg ctcggaatcg cgggatcgcg
     1141 gccacgcatg ttcagcgagg ctgacattgc gctcgccgaa gccttccgag accaggccgt
     1201 gattgcgatc cgcaatactc agctgttcga agaggtccag caacgaaccc acgaattgtc
     1261 gcagtcgctt gaagatttgc gtaccgcaca agatcgatta gtccagaccg agaaactcgc
     1321 gtctctcggt cagctcactg cgggcatcgc ccatgagatc aagaacccgc tcaacttcgt
     1381 caataatttc tccgcgctgt ctgccgaact gatcgacgaa ctggacgaga ccctggcgcc
     1441 agccccgctc gatcagaaga tgcgcggtga catcggcgag ctaacacaga tgttgaagtc
     1501 caacctcgaa aaggtggtcc agcacggcaa gcgcgccgac tcgatcgtca agaacatgct
     1561 gttgcattcg cgcgaaggtt caggcgagca acggccaaca gacataaatg cgcttttgga
     1621 tgagagcctc aatctggcct atcatggtgc acgggctgag aaacctgggt tcaacatcac
     1681 tctgcagagg gacctcgacc ccgttacggg aatggccgac gtctatcccc aggaaatcac
     1741 ccgtgtcctg ctaaacctga tttctaatgg attttatgcc gcgaccagac gtgccgctga
     1801 atccgacgac ggattcgagc caacactgcg ggccgcaacg aaaaatcttg gtgatagaat
     1861 cgaaatacgc atccgcgaca atggcacagg aattgctccg gaggtaaagg agaagatatt
     1921 taaccccttc tttacgacca aaccagctgg cgaaggaaca gggctcggtc tttcaatgag
     1981 ccacgacatc gtggtgaaac aacacggtgg cacgatagac gtcgattctg agccgggcat
     2041 ttttaccgaa tttattatca acctgccgcg agcgacagtc acgcagaagg cggccggagg
     2101 cacgaattga gcacccacat tctcgtcgtc gatgacgagc ccgatgtaga aagcatgttc
     2161 cgccaacatt tccggcgcga tctgcgttcg ggtcgcttca tcatggaatt cgcgctgtcg
     2221 gcaccggctg cactggagcg cgtcagggcc accgccgacc cgtcactgat cttgatctta
     2281 tccgacatta acatgcctgg tatgaccggc ctagaaatgc tgccgaaggt caaggaattg
     2341 cgacccgacg tcccggtgat catgataacc gcctatggcg atgaggacac gaagcggcgc
     2401 gcaatcgccc agggagccac cggattgctg accaagccga tcgatttcgc cttgctgcgc
     2461 catgagatcg acgcgcggct agatcaggcg
//
LOCUS       DATMGX010000394         7584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_131857, whole genome shotgun sequence.
ACCESSION   DATMGX010000394 DATMGX010000000
VERSION     DATMGX010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131857"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VNR65_06350"
     CDS             complement(<1..216)
                     /locus_tag="VNR65_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18331.1"
                     /translation="MEIVMTRLIAATAAAFILMFGYAYAASFLSEQQARASAINILKG
                     DPYGRTTDEVAKNIKQIQFAQDGKTRAC"
     gene            1090..1359
                     /locus_tag="VNR65_06355"
     CDS             1090..1359
                     /locus_tag="VNR65_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HWJ18332.1"
                     /translation="MTGSQGISRRQVLTIAAGASVTGAAAVIGTSTPAQAAKVSQDSV
                     KYQDTPKGEQRCENCIQLEAPSTCKTVDGTVAAQGWCMVYAKKPA"
     gene            1689..3014
                     /locus_tag="VNR65_06360"
     CDS             1689..3014
                     /locus_tag="VNR65_06360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ18333.1"
                     /translation="MTTTATYGTLSASEHSTQLRKAIIASTIGTAIEWYDFFLYGTAA
                     GLIFGKLFFPNSDPLTATLAAFGTYFIGFIGRPIGAAIFGHYGDRIGRKATLIATLLC
                     MGIATFLIAFVPTYETVGIWGAVILTVLRMIQGIGVGGEWGGSVLLAMEWSRHHGHRG
                     LVASWPQFGVPCGLFLANLAVLAFSQLSGDQFAIWGWRIPFALSIILVGIGLWIRLGI
                     FETPVFQALLNDNKIEKTPIIEVIRKQPKQIILSALLRLSEQAPFYIFTAFIFAYAVG
                     TLHMSRNLLLGAVSAASVVSFVTIPLSGHISDRIGRKKMYMIGAATTGVFGFLYFGMV
                     DTAVPVMVFVAIVLSLIPHDMQYGPQAALIAEAFTPRLRYSGASLGYQLASVIAGGPA
                     PLIATALFASFHSGYAISIYIAACAVVSLVATAMMPDYTGKDISMEYDD"
     gene            complement(3721..3843)
                     /locus_tag="VNR65_06365"
     CDS             complement(3721..3843)
                     /locus_tag="VNR65_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18334.1"
                     /translation="MPELAKAVAFVVSTALRAVEVIEREERLPKHAPRSATRHG"
     gene            complement(4098..4223)
                     /locus_tag="VNR65_06370"
     CDS             complement(4098..4223)
                     /locus_tag="VNR65_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18335.1"
                     /translation="MAVFAIVGLLAVLGGVATKNRKLAIVGLIMMGIAMAGRFHH"
     gene            complement(4958..5893)
                     /locus_tag="VNR65_06375"
     CDS             complement(4958..5893)
                     /locus_tag="VNR65_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18336.1"
                     /translation="MASFVQFLQNSGVYAAQSFMTVPRGGFNLVCLRDSKDVGLSYDR
                     DRLQVEDVRFEHVERVAQEYINYRFREQRSQERQDQLLRLRTALYATARPESLARALK
                     VSANGGGIPELRAMRGNTDIRLEVASLPRKDFTIAFKFLRHLDKSGNVINATKWTPDD
                     APWFLTRLNWTFGAQTNIFFTPVDAAWITVQKALGPKISRGVFRKDIVPLKRHGADLT
                     CFLVGDYDAMDNEAAGEYLPDEQVCVVVDQGNPPMFDYGEAFIGVMAHEVGHFLHHKR
                     NMPGSGHHDRSGILLSSGMESLSLDKQLVSNLNAW"
     gene            6037..7095
                     /locus_tag="VNR65_06380"
     CDS             6037..7095
                     /locus_tag="VNR65_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ18337.1"
                     /translation="MSQTPNTAIGIDIGKNSFHVVGHDARGAIVLRQKWSRGQVEARL
                     ANIPPCLIGMEACVGAHHLSRKLASLGHDARLMPAKYVRPYSKGQKNDFNDAEAIAEA
                     VQRPTMKFVATKTAEQLDLQALHRVRERLVSQRTGIINQIRAFMLERGIAVRQGIGFL
                     RTELPTILATRTDALSPRMLRVIEELAGDWRRLDQRIDGLSGEIEALARQDQACSRLM
                     TVPGIGPIISSAMVAAIGTGDVFSKGRDFGAWLGLVPKQISTGDRTILGKISRRGNRY
                     LRVLFVQAAWVVLVRIKNWERYGLKSWIEAAKRRLHHNVLPIALANKLARIAWAVLAK
                     GRAFELTRTDDAGVVQPA"
BASE COUNT         1594 a         2258 c         2191 g         1541 t
ORIGIN      
        1 gcacgccctg gtttttccat cttgggcgaa ttgaatttgc ttgatgttct ttgcgacctc
       61 gtcagtcgtt cttccatacg gatcgccctt caaaatattg atggcactag cgcgtgcctg
      121 ctgttccgaa agaaaggagg cagcataggc ataaccaaac atgagtatga aagctgcggc
      181 ggtcgcggcg ataagtcgcg tcatgacaat ctccaaggcg cactcctgtg cgcacggcgg
      241 gcactccggg aaccaatcgc cgtttccttt taccaagaac gccgatccga ttctttgttc
      301 tcgatcaaat gggcaccatc cgtatccggg cctgtgtttt cttcgcagag tgcggtgcgg
      361 tggcatagaa cgagaacgac gccgctgaaa aatggccggt ttggcactta gctgacgtcg
      421 gtgttttggc ggacgcccgc tttgcgccaa aagctgccct tggaaggctc gcaatccaac
      481 ttccgcttta cacccgaaaa gcagactcaa ttcggaaatc gcggcatgtc tcaaaagtgc
      541 cattaacgga agtagccgct ctcatccgat cacctcatcc gcagtcgcga cctgtgcccc
      601 tggaatatcg agaccgagcg ccttggcaac cccctcctta tctgaagcag tgctggcgca
      661 aaaggcgtta agagcggtta gacgcagtgg cttgtgtgcg cattcggttt tagctttgcg
      721 tctgccgtaa tcggccagga aaccgcagca ccacccaaaa gtacgatgaa ggtgggccaa
      781 cagtcatgtt tgtgagatat tcaccttctg gacctgtaag ccgaggcgga aagactttgc
      841 aagcctccac acattgtgcg cgtcccgcct cttctacagc aattaaacgg gccttacgct
      901 aaaacttaag tggtcaattt tacatagacg cgcgctggtg tcgcgcgtag catccagccc
      961 atactgcgct gcgcgatcaa tcgcagataa aaaccgttgc gacgcgtttg cgctcgacta
     1021 gtgcggcgcc gtgcgaaacg attcgcgatg atgctaactg attgcctctc acagcaggaa
     1081 cgacgtgtca tgacaggatc acaaggaatt tcccggcggc aggttttgac catcgctgcc
     1141 ggggcatcgg tcacaggcgc ggcagcggtc atcggcacat cgacgcccgc gcaggcggcc
     1201 aaggtgtcgc aggacagcgt aaagtatcag gacaccccga agggggagca acgctgcgaa
     1261 aattgcattc agcttgaagc cccatcgacc tgcaaaaccg ttgatggaac tgttgccgcg
     1321 cagggctggt gcatggtcta tgcgaaaaag ccggcataaa atctaacatc cggctgcgat
     1381 cggcgcccta gcgcattcgc aggtttcgaa cgttgaaaag gttcggattt tctgcggctt
     1441 ccctgagcgg tgctagcaaa ctggccctcg ctcgggctgt gccggtggcc gatatccggc
     1501 cattgcaact ttggaccagt gggatgatag ttgctggtct atgcaaagtg ctcaagtctg
     1561 aaacgggtca accgcatcga ttcagcaatg tgcgcgccga ttctgtttgt ccctcaacaa
     1621 cagacgctgg aagtggtcgc taagaaccat ctcccaaaag ggcgaggaaa cgaaccggag
     1681 ggattcaaat gacgacgaca gctacttacg ggacgctgtc cgcgtccgag cacagcacgc
     1741 agctgcgcaa ggcgattatc gcctcaacta ttggtactgc gatcgagtgg tacgacttct
     1801 tcctttacgg cacggcagcc gggctcattt tcggcaagct gttctttccc aattcggatc
     1861 cgctgacggc tacgctcgcc gccttcggaa cctatttcat cggctttatc gggcgcccga
     1921 tcggcgctgc gatcttcggc cattacgggg accgcatcgg ccgcaaagcg acgctgattg
     1981 caacgctgtt gtgcatgggc atcgctacct tcctgatcgc attcgttccg acctacgaaa
     2041 cggtgggcat ctggggcgcc gtcatcctga cggtgctgcg gatgattcag ggcatcggcg
     2101 tcggcggcga atggggcggg tccgttctgc tggcgatgga atggtcgcgt caccacggcc
     2161 accgcggcct ggtggcgtct tggccgcaat tcggcgtgcc gtgcggcctg ttcctggcga
     2221 atctcgctgt gctcgccttc agccagttgt ccggcgatca gttcgccatc tggggctggc
     2281 gcattccctt cgcgctgtcg atcattctgg tcggcatcgg cctgtggatc cgcctcggca
     2341 ttttcgaaac gccggtgttc caagcgcttc tgaacgataa caagatcgag aagacgccga
     2401 tcatcgaggt tatcagaaag cagccgaagc agatcatcct gtcggctttg ctgcggttgt
     2461 cggagcaggc gccgttctac atcttcactg cgtttatctt cgcttatgcg gtcggcacgt
     2521 tgcatatgtc acgcaacctg cttctgggcg cggtgtcggc agcctccgtc gtctcgttcg
     2581 tcaccatccc gctgtcgggc catatctccg accgcatcgg ccgcaagaag atgtacatga
     2641 tcggggcagc gaccacgggc gtgttcggct tcctctattt cggcatggta gacaccgcgg
     2701 ttcccgtgat ggtgttcgtc gcgatagtgc tgtcgcttat tcctcatgac atgcagtatg
     2761 ggccgcaggc ggcgctgatc gccgaggcct ttacgccgcg cctgcgctac agcggcgctt
     2821 cgctggggta tcaactggca tcggtcatcg ccggcggccc agcgccgctg atcgcgaccg
     2881 ccctgttcgc gagctttcat tcgggctacg cgatctcgat ctacatcgca gcgtgcgcag
     2941 tggtgagtct ggtagcgacg gcgatgatgc ccgactacac cggtaaggac atctccatgg
     3001 aatatgacga ctgatacatc actgcgatcg tgagcccggg cgaaccaggc tcctctctct
     3061 ttgtgagccg atgtccgctg tgggtccaga ttgtgtaaaa acgcggatgt ttaggtttgc
     3121 aggcgagccg gctggctcat gatgccgagc ttatgctcgg atcgcttcca ttagtccgcc
     3181 aacgcccaga atgctcatga cccgtttcat gttgtaagcc agggccccgg gaacggacat
     3241 cgtcaggccg acccggtacg tccgacacgt gccatccacg gacatcccga gccagcgccc
     3301 aagcgcggcg cgaaagcgcg gcgtaagcgc aactccggcc acagttcggt gtgctcattt
     3361 agaaacggtt aagccctctc cggcagattg gactctgaag gtgttgaccg gcacggcggt
     3421 agccggtcgc attctggatg gtacatccaa cgctctcgaa tagagccagc ataccgaaaa
     3481 gatcagcgcg gctaatggag ccctgaagga cctcagcctt tgggacgagg acgcgcgagt
     3541 caatgagaca aaatgaacgg aagtccctgg ttcttctgga atgggatcgt tggctcgaaa
     3601 ctcaggctat tgatccaccg agggcaacgg cccgagacac gtttaagttc tattgcgagc
     3661 ttcaagatag gcggtcgcct cttctggatt tccgatcaac cggacgcgac aagtggcaag
     3721 ttatccatgc ctggttgctg agcgaggagc gtgtttcgga agtcgctcct ccctctcgat
     3781 cacctcgacg gcccgcagcg ccgtcgaaac gacgaaggca accgccttag ccaactcagg
     3841 catcgcatgc tcaatgtccg ctcctcctca aaaggcgaaa tactccagcc gaacacatgt
     3901 cgtcctttta gcctgcgaca acggacatgc ggtggctgca ccgcgatgac cgtttcgtgc
     3961 ccacggcgga agtgagaagg atcagtcaat cacttcctca gaccgggcat gcagcttggc
     4021 cggaggcgcc acttagcgga attcccaccg cagcccccgg cgccaatccc ttatcgcgcc
     4081 cctccttgca ggcctcgtca gtgatggaaa cggcctgcca tcgcgatgcc catcattatc
     4141 aggccgacga tagccaattt gcggtttttc gttgccacgc ccccaagaac agccaacagg
     4201 ccgacaattg caaacactgc cattccgcca tcaaatcctt tgctgaccag cgggcaaccg
     4261 cgcaattacc ttgccgagcg aaaaccagag acagggcggc ggcgaatgtt caagccgaat
     4321 gcagacggct gcggcgctct ccttcaccgc gtccacgatg aaaaacgtgg ggtcaccatg
     4381 aactacgaca catccgccgg ggagtttgtt cagtgttttt gaatcgctgt tttgacaggc
     4441 ttcaaaagat gtccaatcgg agcatgcgat accgtcgtag attgccgcta agtgatcgcc
     4501 tggccggacg tcagatgcga ctgcgggaca gccaagggcg agaattgcaa agcaggaagc
     4561 tggaatgcgc atgccttcat tattgatcgt cgaggtaata aacgtattaa cagtcaacga
     4621 gcaatagtcc gttgtgggtc aaactgagaa gaactcggcc tgggcaaaac tcttccgcgt
     4681 tacccctgaa agcggacatt gctctgttcg gtcggcatgt ctcaaaggtg ccataaccgg
     4741 actcatgcgc cgcagcagag tgccgccatt ggtcacctcg tgggatggat agctactcgg
     4801 gcacccctgc tttgcacgac aaactcccgc ggagccacca gggccaccgg cgagcaaacc
     4861 ctacgccagc gcaggcgctt cagcaggcgc tcggcattca aacgaactgc cggcgctgac
     4921 cgtggcctca acgaagggat gccgagaacg agcgctctca ccacgcgttg aggttgctga
     4981 cgagctgctt gtccagggat aagctttcca tgccactgga aagcaaaata ccgcttcggt
     5041 catggtgtcc actcccgggc atgttccgtt tgtggtgcaa gaagtgaccg acctcgtgcg
     5101 ccatgacgcc aataaacgct tcgccatagt cgaacatggg tggattgccc tggtcgacaa
     5161 ccacgcatac ctgctcatca ggcagatact ccccagccgc ctcattatcc attgcatcat
     5221 agtcgccgac gagaaagcac gtgagatcgg cgccgtgcct cttcaatggc acgatatcct
     5281 ttcgaaaaac accccgcgag atttttggcc ccagggcttt ctggaccgta atccatgcgg
     5341 catcgaccgg ggtaaaaaag atattcgtct gagccccgaa cgtccagttc agcctggtga
     5401 gaaaccaggg cgcgtcatcc ggcgtccatt tcgtggcgtt gatcacatta cccgatttat
     5461 cgaggtgcct gaggaatttg aacgcgatcg tgaagtcttt gcgcggcaga ctggcgacct
     5521 ccaacctgat gtctgtgttg ccgcgcatgg ctctgagttc cggaataccg ccaccattgg
     5581 ccgagacctt gagggcgcgc gcaaggctct ccggccgcgc ggtggcatac agcgctgtac
     5641 gcagacgcag cagctgatct tggcgctcct ggcttctttg ctcccgaaag cgatagttga
     5701 tgtattcctg agcgacgcgt tcgacgtgct cgaagcgcac atcctcaacc tgtaaccgat
     5761 cgcgatcata cgataggcca acgtccttgc tgtcgcggag gcagaccagg ttgaagcctc
     5821 cccgaggaac agtcatgaag ctttgcgcag cgtacacacc agaattctgc aagaactgaa
     5881 cgaagctcgc catgacatcg cttccggatt gaactaccgg aagtgtatta tcgatccatg
     5941 ccgagttcaa tgtccgctaa tgggatggtc cggccgtgct cctgccccgc cagcaagcga
     6001 agctggcaag ggggctccaa agacgaggag cacgccatgt ctcagacacc caataccgcg
     6061 atcggcatcg atatcggcaa gaactcgttc cacgtcgtgg gccacgatgc tcgcggcgcc
     6121 atcgtgctgc ggcaaaagtg gtcgcgtggc caagtggaag cgcggctcgc caatataccg
     6181 ccttgcctga tcggcatgga agcctgcgtc ggcgcacatc acctgagccg caaactcgca
     6241 tcgcttggtc acgatgccag gttgatgccg gccaaatatg tccgccccta tagcaaggga
     6301 cagaagaacg acttcaatga tgccgaagcg attgccgaag ccgtgcagcg cccgacgatg
     6361 aagttcgtgg cgaccaagac tgcggaacaa ctggatctgc aggcgctgca tcgggtgcgc
     6421 gagcggctgg tgtcgcaacg caccggcatc atcaaccaga ttcgcgcctt catgctggaa
     6481 cgcgggatcg ccgtgcgcca gggtatcggc ttcctgcgta cggaactgcc caccatcctt
     6541 gcgacgcgca ccgatgcctt gtcgccacgc atgttgcgtg tcatcgagga gttggcaggc
     6601 gactggcgcc ggctggatca gcgcatcgat ggcctatccg gcgagatcga agcactggcc
     6661 cgtcaagatc aggcatgttc gcgcctgatg acggtgcctg gcatcgggcc gatcatttcg
     6721 agcgccatgg tggccgcgat cggcactgga gatgtgttct ccaaaggccg cgacttcggc
     6781 gcctggctcg gactggtgcc caagcagatc tcaacaggag accgcacgat cctcggcaaa
     6841 atctccaggc gcggcaatcg ctacctgcgc gttctgtttg tgcaggcggc atgggttgtg
     6901 ctggtcagga taaagaactg ggaacggtac ggactcaaat cctggatcga agccgccaag
     6961 aggcggttgc accacaacgt gctgccgatc gcgctcgcca acaagcttgc ccggatcgcc
     7021 tgggcggtgc tggctaaagg acgtgccttc gagctgacga ggaccgacga tgcaggcgtc
     7081 gtccaacccg cgtgatcctc gcgccgtgct cggtgcggtc aaggcgcagc ctggcaacgc
     7141 cggagccagc cgcaagccaa gcgcgacggc tggccttgac cgcccctgcg cgcgacgcga
     7201 tcgacgctct gcgggccggg acgaaggaac ggcccttggc tcgaacaaag gaactgcgcg
     7261 atctgaggag caagcgatga cgtaacccta tcaacagttt ccagccgagg tctgcgagag
     7321 gatgagacga gatggtggtt cggtcttccg agcgtgtgcg aacactggtg acccgaatgg
     7381 cccgatcgag gcctgtccgc taatgagaac gcacgcgcgc tgatatccat gatggtccgg
     7441 agcaagatgg ctccaaattg agaccggata cattgatgca agaccgctta ccgccagctc
     7501 ggcaaaatcc acttgcaacg cacggccgga ccatacatcc gggtcaaaag gcgacttcgc
     7561 gtggcgaccg ggctacttcc gctt
//
LOCUS       DATMGX010000395         4338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_386004, whole genome shotgun sequence.
ACCESSION   DATMGX010000395 DATMGX010000000
VERSION     DATMGX010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386004"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3866)
                     /gene="rpoC"
                     /locus_tag="VNR65_06385"
     CDS             complement(<1..3866)
                     /gene="rpoC"
                     /locus_tag="VNR65_06385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWJ18338.1"
                     /translation="MQTQVFDQIRISIASPEKILSWSYGEIKKPETINYRTFKPERDG
                     LFCARIFGPIKDYECLCGKYKRMKYKGVICEKCGVEVTLTKVRRDRMGHIELASPVAH
                     IWFLKSLPSRIGLLLDMGLKDLERVLYFENYVVIEPGLTPLKAGQLLSEEDYLKAQEE
                     HGEDSFQAGIGAEAVRDMLMALNLDQMREQIRQEIAEATTELKPKKLAKRLKVIEAFI
                     QSGNRPEWMIMKVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLIELR
                     APDIIIRNEKRMLQEAVDALFDNGRRGRVITGANKRPLKSLADMLKGKQGRFRQNLLG
                     KRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYSRLDAQGYSATVKQAKKLVE
                     KERPEVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLVEGKAIQLHPLVCAAFNAD
                     FDGDQMAVHVPLSLEAQLEARVLMMSTNNILHPANGGPIIVPSQDIVLGLYYLSLIME
                     KEPGEGRIFGSVAEIQHALAAGNVTLHAKVKGRYVGVDESGNRISKVYETTPGRMIVA
                     EHLPKMPGISFDLVNRLLTKKNIARVIDAVYRSCGQKETVIFCDRVMNLGFHHAYKAG
                     ISFGKDDMLVPDTKPKIIDETEALVLDYEQQYNDGLITRMEKYNKVVDAWAKCTDRIA
                     KEMMDRISAVQYDKATGREKQINSVYMMAHSGARGSEKQMRQLAGMRGLMAKPSGEII
                     ETPIISNFKEGLSVLEYFNSTHGARKGLADTALKTANSGYLTRRLVDVAQDCIVTEVD
                     CGTDKGIKLGAVIDAGEVLVSVGDRVLGRVPADDVVHPSTGQVIVKAGDLIDENEARL
                     IEEAQVQEVRIRSALTCELKNGICSKCYGRDLARGTPVNIGEAVGVIAAQSIGEPGTQ
                     LTMRTFHIGGTAQVADNSFVESNFAGKVKVRNRIAARDSQGRLVAMGRNMAVVILDED
                     GRERATHKIMQGAHLKVDDGDVIKRGQRLAEWDPYSRSIITEIDGVVEFEDLLEGISV
                     NEQSDETTGITNRVIVDWRANPRGSALRPAIVIKDKAGGKVLKLPRGGEARYLLSVEA
                     ILSVEPGQTVKAGDILSRISLESAKTRDITGGLPRVAELFEARRPKDHAIIAEISGTV
                     HFGRDYKNKRRVVIQPDEEGLDPVEYLLPKGKHLAVQEGDRIEKGEFILDGNPAPHDI
                     LAIKGVEELASFLVNEIQEVYRLQGVTINDKHIEVIVRQMLQKVEVTDAGDTELLKNE
                     QVDKLEFDEINVEIEAAGGRPATAVPV"
     gene            complement(4035..>4338)
                     /locus_tag="VNR65_06390"
     CDS             complement(4035..>4338)
                     /locus_tag="VNR65_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18339.1"
                     /translation="EVWALEAYGAAYTLQEMLTVKSDDVAGRTKVYEAIVRGDDTFEA
                     GVPESFNVLVKEMRALGLNVELKQSRVPTIEAQPAQASRPPQFALPRRSRAPQAAE"
BASE COUNT          805 a         1376 c         1211 g          946 t
ORIGIN      
        1 accggcaccg ccgtcgcagg gcgaccaccc gcagcttcaa tctctacatt aatctcatcg
       61 aattcgagct tgtccacctg ctcgttcttc agcaactcgg tatcgcccgc atcggtgacc
      121 tccaccttct gcagcatctg acgaacgatc acttcaatgt gcttgtcgtt gatcgtcacg
      181 ccttgcaggc ggtagacctc ttggatctca ttgacgagga atgaggccaa ctcctccacg
      241 cccttgatag ccaggatgtc atgaggagca gggttgccat cgagaatgaa ctcgcccttc
      301 tcgatgcggt cgccctcctg caccgcgagg tgcttaccct tcggcagcag atactcgacc
      361 ggatcgagac cctcttcgtc gggctggatc acaacgcgcc gtttgttctt gtaatcgcgg
      421 ccgaagtgaa ccgtgccgga gatttccgcg atgatggcgt gatctttcgg acggcgcgcc
      481 tcgaacagct ccgccacgcg cggaagaccg cccgtgatgt cgcgtgtctt cgcgctttcg
      541 agcgagatac gcgagagaat gtcgccggcc ttcacggtct ggccgggctc aacggacagg
      601 atcgcctcca ccgaaagcag atagcgggcc tcgccgccgc gtgggagctt cagaaccttg
      661 ccgcccgcct tgtctttgat cacgatcgcc gggcgcagcg cggagccccg cgggttcgca
      721 cgccagtcca cgatgacacg attggtgata cccgtcgtct cgtccgattg ctcgttgact
      781 gaaatgcctt ccagcaagtc ctcgaactcg accacgccat cgatctcggt aatgatcgag
      841 cggctataag gatcccactc cgccaagcgc tggccacgct tgatcacgtc gccgtcgtcc
      901 actttcaagt gagcgccttg catgatcttg tgagtagcgc gctcacggcc gtcttcgtcg
      961 agaatgacga cggccatgtt gcggcccatg gcgacgagac ggccctggct atcacgcgcg
     1021 gcaatccgat tgcgaacctt caccttgcca gcaaagttgc tctccacgaa ggagttgtcc
     1081 gcgacctgag cagtcccacc gatgtggaag gtgcgcatcg tgagctgcgt acccggctcg
     1141 ccgatggact gcgccgcaat tacgccaaca gcctcgccga tattcaccgg cgtgccgcgt
     1201 gcgagatcgc gtccatagca cttgctgcaa atgccgttct tgagctcgca agtaagagca
     1261 gagcgaatgc gcacctcttg aacctgcgct tcctcaatga gccgcgcctc gttctcgtcg
     1321 atcaggtctc cggccttgac gatgacctgg cccgtgctgg gatgcacaac gtcatcggcc
     1381 ggcacgcgtc cgagaacgcg gtcgcccacc gaaacaagca cctcgccggc gtcgatcacc
     1441 gcgccgagct tgatgccctt gtcggtgccg cagtcgacct ccgtcacgat gcaatcctgc
     1501 gctacgtcga ccaagcggcg ggttaggtaa cccgagttcg ccgtcttgag tgccgtatcc
     1561 gctagacctt tgcgcgcgcc gtgcgttgag ttgaagtact ccagaaccga cagaccctcc
     1621 ttgaagttgg agatgatcgg cgtctcaatg atttcacccg acggcttggc catcagtccg
     1681 cgcatgcctg caagctggcg catttgcttc tccgagccgc gcgccccgga gtgcgccatc
     1741 atgtagacgg agttgatctg cttttcgcgg cccgtcgcct tgtcatattg gacggccgaa
     1801 atacgatcca tcatttcctt ggcgatgcgg tcggtgcact tcgcccaggc gtcgacgacc
     1861 ttgttgtatt tctccatccg cgtgatgagg ccgtcattat attgctgctc ataatcgaga
     1921 acgagcgctt ccgtttcgtc gatgatcttt ggcttcgtat cggggacgag catgtcgtcc
     1981 ttgccgaagg agatgccggc cttgtaggcg tggtgaaagc ccaggttcat gacccggtcg
     2041 cagaaaatga ccgtctcttt ttgaccgcaa gagcggtaaa ccgcgtcgat cactcgcgcg
     2101 atatttttct tggtcagaag cctgttcaca agatcgaagc tgatccccgg catcttcggc
     2161 agatgctcgg cgacaatcat ccgccccggc gtggtctcgt acaccttgga gatccggttg
     2221 ccagactcat cgacgcccac gtagcggccc ttgactttcg cgtggagcgt gacattgcct
     2281 gccgccagcg catgctggat ttcagccacg ctcccgaaga tgcgcccttc gcccggctcc
     2341 ttctccatta tgagggacag gtagtaaaga ccaagcacga tatcctggct cggcacgata
     2401 atggggccgc cgtttgccgg atgcaggatg ttgtttgtcg acatcatcag cacgcgggct
     2461 tcgagctgcg cttcgaggct gagcggtacg tgaacggcca tctgatcgcc gtcaaagtcg
     2521 gcgttgaacg ccgcgcaaac cagcggatga agctggatcg ccttgccttc aacgagaacc
     2581 ggctcgaacg cctgaatgcc gagacgatga agcgtggggg cgcggttgag cagcaccgga
     2641 tgctcgcgga tcacctcgtc gaggatatcc cagacctccg gacgctcctt ctccaccagc
     2701 ttcttcgcct gcttcacggt cgccgagtag ccctgcgcgt caagccgcga atagatgaac
     2761 ggcttgaaga gttccagcgc catcttcttt ggtaggccgc actgatgcag cttcaactcc
     2821 ggaccgacca cgatcacaga acggccggaa tagtcgacgc gtttgccgag caggttctga
     2881 cggaaccggc cttgcttgcc tttcagcatg tcggcaagcg acttcagcgg acgcttgttt
     2941 gcgcccgtga tcacgcgacc acggcggcca ttgtcgaaca gcgcatcgac agcttcctgc
     3001 agcatgcgct tctcgttccg gataataatg tccggggcgc gcagttcgat aagccgtttc
     3061 agacggttgt tgcggttaat gacgcggcga taaagatcgt tcaggtcgga cgtcgcgaag
     3121 cggccaccgt ccagcggaac gagcgggcgc aactccggcg gaatcaccgg aatgaccttc
     3181 atgatcatcc attccggtcg gttgcccgac tggatgaaag cctcgataac cttcagacgc
     3241 ttcgccagct ttttgggctt gagctcggtt gttgcctccg cgatctcctg gcggatctgc
     3301 tcgcgcatct ggtcgaggtt aagcgccatg agcatatcgc gtacagcttc cgcgccgatg
     3361 ccagcttgga acgagtcctc gccgtgctcc tcctgcgcct tgaggtagtc ctcctccgac
     3421 aggagttgcc ctgccttcag cggcgtgagc cccggctcga tcaccacata gttctcgaaa
     3481 tagagcacgc gctccaggtc cttcaaaccc atgtcgagca gaaggccgat gcgcgatggc
     3541 agcgatttca agaaccagat gtgcgccact ggcgaggcga gctcgatgtg ccccatgcgg
     3601 tcgcggcgca ctttcgtgag cgtcacttcc acgccgcatt tttcgcaaat aacgccttta
     3661 tacttcatgc gcttgtattt gccgcaaaga cattcgtagt ctttgatcgg gccaaaaata
     3721 cgcgcacaaa acaaaccgtc acgttcgggc ttgaacgtac ggtaattgat ggtttcgggc
     3781 ttttttattt caccgtaaga ccaggagagt atcttctccg gacttgcaat agagatgcga
     3841 atctggtcaa atacctgagt ctgcatctgc gggctgaaga gattcgtgat ctcttgattc
     3901 attcgtcgct cctatgagcc gcccccgcgg cttcttcgac ccaatgtccg cttggcggac
     3961 agcgaggctt ttcacctcgc atagttcctg aaacgccgtt tgccggcacc ggctgagccg
     4021 atacccgctt ctgtttactc cgcagcctgc ggcgctctcg accttcgcgg cagcgcaaac
     4081 tgtggaggcc gcgatgcttg cgcgggttgc gcctctatcg tcggcactcg gctctgcttc
     4141 agctcgacgt tcaagccgag cgcgcgcatc tccttcacaa gcacgttgaa gctttccggc
     4201 acgcccgcct cgaaagtgtc gtcgccgcgt acgatggcct cgtagacctt ggtccggccc
     4261 gccacgtcgt ccgacttcac cgtcagcatt tcctgcaagg tgtaggcggc tccataagct
     4321 tcgagcgccc acacctcc
//
LOCUS       DATMGX010000396         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_406345, whole genome shotgun sequence.
ACCESSION   DATMGX010000396 DATMGX010000000
VERSION     DATMGX010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406345"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VNR65_06395"
     CDS             complement(<1..762)
                     /locus_tag="VNR65_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HWJ18340.1"
                     /translation="MLAQSIIDPTRFAIIGLIIGVLIAAPVGPVNIVCIQRTLERGFW
                     GGFAAGLGAVLADGLIATVAAYGASAISGFMRDHQLKIELIGGFIMTGFGLNVFLAQP
                     KIVATATTNIARLRRLSSIIPRPLRPALRLKVWRIMPHLSIVPQTFFLTITNPGAILG
                     LFALFGGATSVLGGIDNWIQAITVVTAIMAGSLMWWAGLSRLIARVRHKITESRLKRI
                     NQVSGAVLVMFGAVLFVQFAYGLAGHRMAGWREPAS"
     gene            911..1498
                     /locus_tag="VNR65_06400"
     CDS             911..1498
                     /locus_tag="VNR65_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18341.1"
                     /translation="MKTLPSAIFFNGLKRVRNAICAFAAALVLGTAMFAATASIAEEV
                     QLGRDVSKLPSEVQRMRQAILQAVMSGDIEELRIPIDMNELHPIFAKDRTPDPIAYLK
                     SVSADGNGREMLAILFNLLTTGYAIKNAGTKNEMIVWPYHAAIPLSGLTPGQEVEIYR
                     FLPPARVKEMTAAGRYSYYSVGIGRDGVWHYFSRE"
BASE COUNT          699 a          802 c          785 g          601 t
ORIGIN      
        1 cgaggccggc tcccgccatc ccgccatccg atgtccggcc aagccatagg cgaattgcac
       61 gaacagcacc gccccgaaca taaccagcac cgcgcctgag acctggttga tccgcttcaa
      121 ccggctttcg gtgatcttgt gccgcacgcg agcgatcagg cgcgacagac ccgcccacca
      181 catcaggctg cctgccatga tggccgttac cacggtgatc gcctggatcc agttgtcgat
      241 gccacccaac accgatgttg cgccaccgaa aagcgcaaac agcccaagga tggcgcccgg
      301 gttggtgatg gtcaggaaaa aggtctgagg cacaatgctc agatgcggca tgatccgcca
      361 gactttgagt cggagcgccg gccgtaacgg tcgaggtatg atgctcgaaa gacgtctcag
      421 ccgcgcaatg ttcgtcgttg ctgtagcgac aatcttcggc tgcgcgagaa aaacgttgag
      481 accgaagccg gtcatgatga agccgccaat gagctcgatc ttgagctggt gatcgcgcat
      541 gaagcccgag atggcggaag cgccataagc tgctacggtt gcgataagcc catcggcaag
      601 gacagcgcca agaccagccg cgaagccgcc ccagaaacca cgctcaagcg tccgctggat
      661 gcacacgata ttgaccggac cgacaggcgc ggcgatgagc acaccaataa tgaggccaat
      721 aatggcaaat ctcgtcggat cgatgatcga ctgtgccaac attgaccttc tgagccctca
      781 tagtctgaat gcgcaagacg cgacctactg cgccattatc acgcctaaaa atggaagaga
      841 ggtgcatgga ctcagcaccg aagatccatc catgccgtga aacggtcatg ccttgcaaca
      901 ggagacgaat gtgaaaacgt tgccaagcgc aattttcttc aacggcctga aacgcgttcg
      961 caacgccatc tgcgctttcg ccgcagcctt ggtgctcgga accgctatgt ttgcggcaac
     1021 ggcgtcgatt gcggaagagg ttcagctagg gcgcgacgtt tcgaagcttc cctccgaggt
     1081 gcagcgcatg cggcaagcca tcctgcaagc ggtcatgagc ggagatatcg aagagcttcg
     1141 catccccatc gacatgaacg agcttcaccc gattttcgcc aaggaccgca cgcccgaccc
     1201 gattgcctac ctgaaatcgg tctcggccga cggcaatggc agggagatgc tcgccatcct
     1261 tttcaatctc ctcacgaccg gctacgcgat caagaatgcg gggacgaaaa acgagatgat
     1321 cgtctggccc tatcatgcgg ccattccctt gagtggactt accccgggcc aggaagtcga
     1381 aatttacagg ttcttgcccc cggcgcgcgt gaaagagatg accgcggcgg ggagatattc
     1441 ctattacagc gttggtatag gccgggacgg cgtctggcat tacttctcgc gcgagtagca
     1501 tgcgctcccg aatgctgtcg cccgaagcac gcgttaaata ttcccgctct gattgcttcc
     1561 gaaaaaaagc cgcgccccga aggccgggcg cggcaaaact ctcatgcctg aatacctcaa
     1621 tatagcgtag caggcctcgc ggacgccaca acattttgtg tttctgcgac actttgtaca
     1681 gttgcgagtg aaataagctg ctgaatgaaa gagaggcctc tggcgtttgt cgcggcgcgc
     1741 tcgaggaacg gatcagccaa gctaccaata atcagaacag gctggtgaga cagcctcgac
     1801 cttcactgtt ctagtcataa caagattagc gccggctata aaacttaata gtgatccctg
     1861 gcccgcaaca caattaaatg attacaggtc attcattcag ttgatgccgc attgttgact
     1921 gagtgataag agattctgtt ctgggttttt gttctgcaca taagttcaag ctgaacaaga
     1981 ccaatgtata gaaagtaaat aaacgggaga tccgtttaac tctgatccat tgctcatggt
     2041 ctggaactaa gttcctcaag acggaacgtg cgaatagcgc ccagaaaagg tagatgggat
     2101 tcattggctt ctcccgcagc gaggagaata aatatgagaa cctttataac gatagtgtgc
     2161 ggcggtttgc tggctcttac ctgcgcaaca gcatcggacg ctagacaaca cgccaagcag
     2221 gtgcgtccaa cgcaaagcga gcccatctac aactcggatt acaacaatac gcgtggccgg
     2281 gatagttcct gctttaattc taccggtctt cccgaattgt atgcgtgctc cggaaacggg
     2341 ggcggctgag cgctcgactt aaaccgcgcc ttcaaaggcg cggtttttta agaccacaca
     2401 cactgactaa gctagcgtat ggagactgct atgcgttcca taatcgcgac tattattgcc
     2461 atttcttctt tctcaatttg cactgttgcg gtggttcagg ctcaagagat acccgaatac
     2521 aggatcatcg cccatggcct ccaagatgga cgtgcggagc ggtctcggga ttacttcaac
     2581 caggaagtaa gccggctata catcaaccgg gacacgaatt gcatcgtatt ggggcatcaa
     2641 atctatcctt gtgagtgatc ataaggctgc ctcggcttcg ggccggggta tcagccttgg
     2701 gccagcgacc gaattccggg cgcgcaagaa gctcttcgag gttgtcaggc ggtcgtgctg
     2761 tcgtaagcag cctgctgaag gcggcgaatg tccggaatga ggatctcgcg gtaattcatc
     2821 agctcgataa caccttttgt tttcagccgc gtaatctgtc ggctgaccgt ctcgatggta
     2881 aggccga
//
LOCUS       DATMGX010000397         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_67228, whole genome shotgun sequence.
ACCESSION   DATMGX010000397 DATMGX010000000
VERSION     DATMGX010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67228"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..769)
                     /locus_tag="VNR65_06405"
     CDS             complement(71..769)
                     /locus_tag="VNR65_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ18342.1"
                     /translation="MSTTEVAAKTGAVSVQNVRKFYQRDSMQIKVLDGLNLEVPPGEF
                     LALMGPSGSGKTTLLNLIAGIDRATSGRVVVAGTDLGPLSEGELAKWRSRNVGFIFQF
                     YNLIPVLNAVENVELPLLLTSLSRRERRERAMTALRIVGLSDRYKHYPRQLSGGQEQR
                     VAIARAVVTDPTVLVADEPTGDLDHQSAEEILDLMQTFNKDFGKTIVMVTHDPRAAVR
                     AHTQRHLEKGVLTS"
     gene            complement(784..1986)
                     /locus_tag="VNR65_06410"
     CDS             complement(784..1986)
                     /locus_tag="VNR65_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWJ18343.1"
                     /translation="MRDLKDELASLKIERDTPRRGRWRWLVVLLLLAIVAAGGLYFVR
                     ARPAFGAVEVQTVQPSVQSGSGPMAGTPILTASGYLVARRQSVVSSKIQGRISELRVE
                     EGSVVKTGDVLAKLENADYEAGIAKAKADIEYAKADLAEAQRQERLQEVLFRDKVVSQ
                     DALDTAKAKVALAAATIEQDKAALQMQQANYDFTTIRAPFAGIVVKKMTEAGESVAPI
                     PPGVNISTSSGAIVAIADMNSLEAEVDVNEANVAQLQSGQPAEITVQAIPNHTYKGVL
                     RQVIPTADRTKATVTVKTSILDKDKYLKPEMSCNVTFLQPQKRAEKTTAAPKRIVTIP
                     KDAIVTRDGKSVVFQVEDKKVHQLPVVTGADLRGQVIVTSGLVGSETLVSNPPEKLRD
                     GDSVKVKS"
BASE COUNT          370 a          669 c          617 g          438 t
ORIGIN      
        1 gccagcacgg tgctgagggt gcggagccag ttcttgcgaa gatgcttgag aatgagcggt
       61 gcgaatttca tcaggaggtc agcactccct tctccagatg gcgctgggtg tgcgcacgca
      121 ccgcggcacg cgggtcgtgg gtcaccatca cgatggtctt gccgaaatcc ttgttgaagg
      181 tctgcatcag gtcgagaatt tcctccgcgc tctgatggtc gaggtcgccg gtaggctcgt
      241 cagcaaccag caccgtcgga tcggtgacca cggcgcgggc gatggcgacg cgctgttcct
      301 gtccgccgga aagctgccgg ggataatgct tgtaacggtc ggacaatccc acaatgcgca
      361 acgcggtcat ggcgcgctcg cggcgctcac gcctggatag ggaagtgagc agcaagggca
      421 gctcgacgtt ctccaccgcg ttcagcactg gaatgaggtt gtagaactga aagatgaagc
      481 cgacattccg gctgcgccat ttggcgagct caccttccga cagcgggcca aggtcagtcc
      541 cggcgaccac gacccggcct gaagtggcgc ggtcgattcc cgcgatcagg ttcaacaacg
      601 tggtcttgcc cgagccggat ggccccatca gcgccaggaa ttcgcccggc ggcacttcga
      661 gattcaggcc gtccagtacc ttgatctgca tactgtcgcg ttgataaaac ttgcgaacgt
      721 tttgcacgct caccgcgcca gtcttggccg ccacttcggt tgtgctcatg atctctccaa
      781 tgtctaactc ttcaccttca ccgaatcgcc gtcccgcagc ttctccggtg gattactcac
      841 cagcgtctcc gaccccacca gcccgctggt gacgatgact tggccgcgca gatcggctcc
      901 cgtcaccacg ggaagctgat ggaccttctt gtcctcgacc tggaacacta ctgacttgcc
      961 gtcacgcgta acgatcgcgt ctttcggaat ggtcacaatg cgcttgggcg cggccgtcgt
     1021 tttctccgct cgcttctgcg gctgcagaaa cgtgacgttg cagctcatct ccggcttcag
     1081 gtacttgtcc ttgtcgagga tcgaagtctt caccgtcacc gtcgccttgg ttcggtcggc
     1141 ggtggggatg acctggcgca gcactccctt ataagtatgg ttgggaatgg cctgcaccgt
     1201 aatttccgcc ggctggccag actgaagctg agccacgttg gcctcgttca cgtccacttc
     1261 cgcttccagc gaattcatgt cggcgatggc aacgatggcg cccgacgacg tcgagatatt
     1321 tacgcccgga ggaatgggag ccacgctctc gcccgcctcc gtcatcttct tcaccacaat
     1381 gccggcgaac ggcgcacgaa tggtggtgaa gtcgtagttg gcctgctgca tctgcaaggc
     1441 ggccttgtcc tgctcgatgg tagctgcggc gagggcgacc ttggctttgg cagtgtcgag
     1501 cgcgtcctgc gaaaccacct tgtcgcggaa gagtacctcc tgcaaacgct cctgccgctg
     1561 cgcttccgcc agatcggcct tggcatactc gatatcggcc ttggctttgg cgatgccggc
     1621 ttcataatcc gcgttttcca gcttcgccag gacatcgccg gtcttcacca cgctgccctc
     1681 ttccacacgc aactcggaga ttcgtccctg gatcttcgac gacaccaccg attgccggcg
     1741 cgccacgaga taaccggagg cagtaaggat aggcgtgccc gccatcggcc cgctgccgct
     1801 ttgcacgctg ggctgcacgg tttggacctc gacggcaccg aaggcgggcc gggcccgcac
     1861 gaagtacagt ccgccagcgg cgacgatggc aagaagcagc aggacaacca gccagcgcca
     1921 tcttccccgg cgcggcgtgt cgcgctctat cttcagcgac gcgagctcat ctttcaggtc
     1981 ccgcataaac ggtgtccaac tctccttgga cgatgccact agcatcgcat tgttgccgcg
     2041 agtttggaag agcatgaccg ctgattacta cgttccgggc gcggtttagg tccc
//
LOCUS       DATMGX010000398         3464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_389142, whole genome shotgun sequence.
ACCESSION   DATMGX010000398 DATMGX010000000
VERSION     DATMGX010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389142"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /gene="truB"
                     /locus_tag="VNR65_06415"
     CDS             complement(<1..641)
                     /gene="truB"
                     /locus_tag="VNR65_06415"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWJ18344.1"
                     /translation="MPLNGVIVVDKPAGWTSHDVVAKSRRILGERSIGHLGTLDPMAT
                     GVLPLVVGRMTRLCQFYSSSEKAYQGTIRLGVATDTYDCDGDPVGPAQDVNVTLEEIR
                     AVVSGFVGFIQQTPPSFSAKKIKGVPAYKLARKKQEVELQPVEVEVKEFTVLGLNGDL
                     VDFRCRVSSGTYVRSLAHELGQKLGIGAHLASLRRTAVAEFAIEESHTLDEIG"
     gene            763..1512
                     /locus_tag="VNR65_06420"
     CDS             763..1512
                     /locus_tag="VNR65_06420"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWJ18345.1"
                     /translation="MIPLKDDAPRVGTPYVTYVLLALNTVAFLLSLNWQLRPPEPSPE
                     VLTLLGVVPARLTVVLFNHGYVPWDLISGLGMRYVSTTEAILPFFTSMFLHGGWLHLI
                     FNMWALWIFGDNVEDYLGHSLYLSLYFVCGIAAALLHTLFNIGSTVPSVGASGAIAGV
                     MGAYFLLFPRARVLTLVPFFFVFFMWLPAWIVLGYWFVAQFLSGAATSIAAHSGNSTG
                     IAFWAHVGGFLAGMLLIKVFPARTRRYHYGL"
     gene            complement(1661..2482)
                     /locus_tag="VNR65_06425"
     CDS             complement(1661..2482)
                     /locus_tag="VNR65_06425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ18346.1"
                     /translation="MAAKKPQSNSKRKSSARVPASQPTSPRLLGKVAVVTGGSRGIGY
                     AVASTLAAEGCSVVITGRDGPKLAKSAEELRRLLPRKSRAHDDNDAQIVAEVCDVRDP
                     DSVASLFAIVKRRFGKIDVLVNNAGTAQPAVSIEQTSLAIWRDAIDTNLTGLFLCTRA
                     ALPLMQAGATIINNLSVAARTVFPNFAAYNASKHGALGFTLSLRDELIPRGIRVVALM
                     PGATDTDLWQQFWPDAPRERMVDVESVAQAVLYAVLLPPNANLSELVLTPTGGAL"
     gene            complement(2486..3103)
                     /gene="folE"
                     /locus_tag="VNR65_06430"
     CDS             complement(2486..3103)
                     /gene="folE"
                     /locus_tag="VNR65_06430"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWJ18347.1"
                     /translation="MNPGSMMRDLTETPTLTSASFEELAREMLVRLGEQPDREGLLKT
                     PERFSQALQYFTKGYSEDPEQILKAALFSTNYDEMVIVKDIEMFSLCEHHVLPFFGKV
                     HVAYIPKGKVIGLSKVPRLVEVFARRLQVQERLTVQIAETIQKAIQPLGVGVVIEARH
                     LCMMMRGVEKQHSATVTSSMLGAFRAQQTRQEFLSLIHAKTGNGI"
     gene            complement(3100..>3464)
                     /locus_tag="VNR65_06435"
     CDS             complement(3100..>3464)
                     /locus_tag="VNR65_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWJ18348.1"
                     /translation="GLSEPENQATYGKCNNPHGHGHNYALEVTVSGQVEPGTGMICNL
                     SDLDRSVQSGILERFDHENLNCRPEFDGLVPTTENLSEVIFNILKQSFTAAHLEKVRL
                     EETMMNSFEYAGGAELRV"
BASE COUNT          640 a         1030 c         1010 g          784 t
ORIGIN      
        1 tcgccgattt cgtccaatgt atggctctcc tcgatggcga actcggcaac cgccgtccgg
       61 cggaggctcg cgaggtgggc tccgatgccc agcttttgcc ccagttcgtg ggccaggctg
      121 cggacatagg tgcccgacga aacccggcag cggaaatcga ccaggtcgcc gttcaggccc
      181 aggaccgtaa attccttcac ttccacctcg accggctgaa gctcgacttc ctgcttcttg
      241 cgggccagct tgtaggccgg gacgcccttg atcttcttgg cggaaaacga tggcggagtc
      301 tgctggatga agccgacgaa gccggagacc acagcccgaa tttcttcaag tgttacatta
      361 acatcctgcg ctggtccgac cgggtcaccg tcacaatcgt aggtgtcggt ggcgacaccc
      421 agccggatcg tcccctggta agccttctcc gaggacgaat agaactggca gagccgtgtc
      481 atccgcccaa cgaccagcgg gagcactccg gtcgccatgg ggtcaagggt tcccaggtgg
      541 ccaatggagc gttcgcccaa aatgcgccgt gatttcgcca ccacatcgtg cgaagtccat
      601 cccgcaggtt tatccaccac gatcacacca ttgagcggca tccttctgct atcgtgtagg
      661 ttcaacggtg gtttggtcaa atcgccggcg agaaatcggc tgccgcaagg agtttgaagg
      721 gaattcagtt ccctgccgtg cggtgcgagc gacccacttc ctatgatccc gctgaaagac
      781 gacgcgcccc gggttggcac tccgtacgtg acttatgtgc tgctggcgct gaacaccgtg
      841 gcattccttc tgtctctgaa ctggcagttg cgtccgccag agcctagtcc agaggtcctt
      901 accttgctag gcgttgtgcc cgcacggtta acggtcgtgc tcttcaacca tggatacgtg
      961 ccgtgggacc tcatctcggg tctcggcatg cgctacgtct caactacgga ggccatcctg
     1021 ccattcttca cctcgatgtt cttgcacggc ggatggctgc acctcatctt caacatgtgg
     1081 gcgctgtgga tcttcggcga caacgtggaa gactatctcg ggcattcgct ctacctgtcg
     1141 ctttacttcg tctgcggaat tgcagcggct ttgctgcata cactgtttaa cattggctcg
     1201 acagtgccaa gcgtcggggc cagcggagcg attgctgggg tcatgggagc gtacttcctg
     1261 ctgtttccgc gggcgcgcgt gctgaccctg gtgccgttct tcttcgtctt cttcatgtgg
     1321 ttgccggcgt ggattgtgct cggctactgg tttgtcgcgc agttcctaag cggagccgcc
     1381 acctcgattg cggcgcacag cggcaactcc actggaattg ccttctgggc ccacgttgga
     1441 ggcttcctgg caggcatgct gctgattaag gtcttccctg cccggacgcg gcgatatcac
     1501 tacgggctgt gatattcgca tttcgctgtt cgcatttcgc tgttcgctat ttggttgcgg
     1561 ccagcttcag ttctctggga tcgaatggca tggagacgca actccaggtg ttcgactaaa
     1621 gccaagagcg aaaggcgaat agcgaaaagc ggacttctcg ctacagcgcc ccgccggtcg
     1681 gcgtcagaac taactccgac agattcgcgt tgggaggaag aagcacggcg tagagcacgg
     1741 cctgggcgac gctctccacg tcaaccatac gttcgcgcgg agcgtcgggc cagaattgct
     1801 gccacaaatc ggtatcggtt gcgcccggca tcagcgcgac cacgcgaatg ccgcggggaa
     1861 tcagttcatc gcgcagggac aaggtgaagc ccagcgctcc gtgcttggag gcgttgtacg
     1921 cggcgaaatt gggaaaaacg gtcctggccg cgaccgagag gttgttgatg atggtcgcgc
     1981 cggcctgcat aagaggcagc gccgcccggg tgcacagaaa taatcccgta aggttggtat
     2041 cgatcgcgtc acgccagatt gcaaggctgg tttgctcgat ggagactgcc ggttgagcag
     2101 ttcctgcatt gttcaccagc acatcgatct tgccgaagcg ccgtttcact atcgcgaaaa
     2161 gcgatgcgac tgaatccgga tcgcgcacgt cgcaaacctc ggcgacgatt tgagcatcat
     2221 tgtcgtcatg cgcgcgtgat tttctcggga gcaaccgcct gagttcctcc gcgctcttgg
     2281 cgagcttggg accatcgcga ccggtaatca ccacgctgca accttcggcg gcaagcgtgc
     2341 tcgcgacggc atagccgatc ccccggctgc cgccagtgac aacggctacc ttgcccagca
     2401 gtcgaggact ggtcggctgc gatgccggga ccctggcgct ggatttcctt ttgctattgg
     2461 actgcggctt cttcgcagcc atggattaga ttccgttgcc ggtcttggcg tggatcagcg
     2521 acagaaactc ctggcgcgtt tgttgcgcgc gaaaagcgcc gagcatggac gaggtgactg
     2581 tggccgaatg ttgcttctcg accccgcgca tcatcatgca gaggtggcgg gcttcaatga
     2641 ccactccgac gcccaagggc tgaatcgctt tttgaatcgt ctccgcaatt tgtacggtca
     2701 gacgctcctg cacctggagt cgccgggcga atacttccac caggcgcgga accttgctca
     2761 gaccgatcac tttgcccttg gggatgtagg ccacgtggac cttgccgaag aatggcagca
     2821 cgtggtgctc gcaaaggctg aacatttcga tgtccttcac gatcaccatc tcgtcgtaat
     2881 tcgtgctgaa gagcgctgcc ttcaggatct gttccgggtc ttcgctatag cctttggtaa
     2941 agtactgcag agcttgggag aagcgctccg gcgtcttcag cagtccctcg cgatcgggct
     3001 gttcgcccaa gcggaccagc atctcgcgcg caagctcttc gaagctggcg ctggtcaggg
     3061 tcggcgtttc cgtcaggtct ctcatcatgc tcccagggtt catacccgca attccgctcc
     3121 gcctgcgtat tcgaagctat tcatcatggt ttcttcgagc cgcacctttt caagatgagc
     3181 ggccgtaaag ctttgtttca aaatgttgaa aatgacctcg ctaagattct ctgttgtcgg
     3241 tactaatcca tcgaactcag ggcgacagtt gagattctcg tgatcgaagc gttcaagaat
     3301 cccactctgt acagagcgat caaggtccga aaggttacaa atcattccgg tcccgggctc
     3361 aacctgcccg ctcaccgtga cttcgagtgc atagttgtgc ccgtggccgt gcgggttatt
     3421 gcacttgccg taagtcgcct ggttctccgg ctcggaaagc ccgt
//
LOCUS       DATMGX010000399         2588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_266213, whole genome shotgun sequence.
ACCESSION   DATMGX010000399 DATMGX010000000
VERSION     DATMGX010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266213"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            971..1906
                     /gene="fmt"
                     /locus_tag="VNR65_06440"
     CDS             971..1906
                     /gene="fmt"
                     /locus_tag="VNR65_06440"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWJ18349.1"
                     /translation="MTLRVVFMGTPDFSVPTLSEIVGAGHDVVAVYTQPPRPAGRGME
                     ARKSPVHRFAEEAGLPVFVPKTLRRVNVQNEFTALGADVAVVVAYGLILPKVILDAPP
                     LGCLNLHGSLLPRWRGAAPIQRAIMAGDTETGVMVMKMDEGLDTGPVALADRLSIGPD
                     STAGEIHDHLALMGASLMLRALTHLEKRKLTFTPQPLEGVTYAAKISKEETRIDWSKP
                     APDVHDMIRGLSPYPGAWFEALLGGKAERVKVLRSVVVPGKGEPGLLLDAHLTVACGG
                     SQAVRLTQVQRAGRRPVGGAEFLRGFPLARGTHFG"
     gene            2010..>2588
                     /locus_tag="VNR65_06445"
     CDS             2010..>2588
                     /locus_tag="VNR65_06445"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HWJ18350.1"
                     /translation="MSTLSSIGILVVAGFLSVANASAEAAGDSSSKNASVIELFTSQG
                     CSSCPEADQLLNRLARRPDVIALSFPVSYWDYLGWKDTLARPENTERQRSYAKILGDG
                     EVYTPQAIVNGMQNCLGNSLADIESAVKATVPLVGKEAVPLSVRHEGDKLIIDAGGAP
                     EGSRHKKGKVWVAAVRHSLTVRITRGENAGSAL"
BASE COUNT          513 a          752 c          768 g          555 t
ORIGIN      
        1 tgcaagcctt cccctcattt acggagggtc ggcatccgca ttaacgcttt cgaggcctgc
       61 tcagagttca ctcacgttac ggcccgtcag attgctcgac cgccctaggc ggcctttgtc
      121 acagggcttc aacctagcca gttgcccggc taagtcgcct gtcagctacc ggagctaacc
      181 aacaagtctc cgggtggaac cttgctccac tggtcgtccg cgccctcgtg gcgcacttaa
      241 ttcgaagatg gttaattttc gcgcgccgga attgattctg gtccaaatcg tcttaacaat
      301 tggtttatcc ggcgcaagat ggggaagcgt gcgcctttga ctggacgcgg ataagacact
      361 gaaaaaacac aacctttttc gacaatcgac cccattaaat ggaatttaat ggcggaattt
      421 ttccctttac tgggacgcgc taaagtccgt taaatatgtt tccgttgtgc ctgttaacca
      481 tgtccgcgcc tgtcgcagcc gcgcggcgcc gtcatcctct ctcaatgtat tccatacaaa
      541 tcaaagactt atggcaatta acctctcgtc aaagcgcgtt ccgctaagat ctcgctcagc
      601 cagaatggca gctcgttttc gttgaatgcc gcactcgatt ttggtccaaa gccaacatta
      661 ttttggacca ttgtctggtc catggggaaa tggccatgca gattttcagt atccgatgcc
      721 aaagcaaagc tcaccgcgct cgagcgccgc gccgaggctg aacttgacgt accaactgtt
      781 cggcccggcg agcgtgaggg ttggcgcaag gtctgattgt tggccgggtc aaagcccggc
      841 catgacacat aaaagccggt ccacttaggc cgatacggct ctaactaacc acctttccgg
      901 cttgcaaaag ccggttactg gggctttaac acgaccttca ttcacggctg gcacggggag
      961 gctctcatcc atgacgctgc gggttgtgtt catgggaacc ccggatttct ccgttccgac
     1021 actcagcgaa atcgtcggag cggggcatga cgtcgtggct gtctacaccc agccgccccg
     1081 gcctgccggg cgggggatgg aggccagaaa gtctccggtg cataggttcg ccgaagaggc
     1141 ggggctgccg gtgtttgtgc cgaaaaccct tcgccgcgtc aatgtccaga acgagttcac
     1201 ggcgctcggg gccgatgtcg ctgtcgtcgt cgcctatggc cttatcctgc cgaaagtcat
     1261 cctggacgcg ccgccgctcg gctgtctcaa tcttcatggc tcgctcctcc cgcgctggcg
     1321 tggcgccgct ccgatccagc gagccatcat ggcgggcgac acggagaccg gcgtcatggt
     1381 gatgaagatg gacgagggcc tcgacaccgg gccggtcgct cttgccgacc gcctctcgat
     1441 cggtcccgat tccaccgctg gagagatcca cgaccatctt gcgctgatgg gagcgtcgct
     1501 catgctgcgg gcactcaccc atttagagaa gcggaagctg accttcacgc cgcagcctct
     1561 cgagggcgta acctatgccg cgaagatctc gaaggaggag acacggatcg actggagcaa
     1621 gcctgccccc gacgttcacg acatgatccg gggcctttca ccttatccgg gggcctggtt
     1681 cgaagcgctg cttggaggca aggccgagcg cgtcaaggtg ttgcgttcgg tggtcgtgcc
     1741 gggaaaaggc gagcctgggc tgctgcttga tgcgcatttg acggtcgcgt gcggcggctc
     1801 ccaggctgtg cgcttgacgc aggttcagag agccgggagg cggcctgtgg gaggcgcgga
     1861 atttctgcgc ggcttccctc tcgcaagggg tactcatttc ggataggcga tgacagaaag
     1921 ttgcgcaggg aaaaagcaga cgtgactgga cgttcaatgc agcctcccgg acagtaactt
     1981 tccatggaac catcggattg gagtcaattt tgagcacgct ctcgtccatc ggaattcttg
     2041 tggtcgcggg tttcctttca gtcgcaaatg cttcagccga agcagccggt gacagttctt
     2101 cgaagaacgc atcggtgata gagcttttta caagccaggg atgttcgtcc tgtcccgaag
     2161 ccgatcaact cctgaaccgg cttgcccggc ggccggacgt gatcgcgctg agcttccccg
     2221 tcagctattg ggattacctc ggctggaaag atacgcttgc gcgccccgag aataccgagc
     2281 ggcagcgtag ctacgccaaa atcctgggtg acggcgaagt ttatacgccc caggccattg
     2341 tcaacggcat gcagaattgt ctcggcaata gcctcgctga tatcgagagc gcggttaaag
     2401 ctactgtgcc attggtcggc aaggaagcgg tgccgctcag cgtgcgtcat gaaggggaca
     2461 agcttatcat cgacgccggc ggcgcgcccg aggggtcacg gcacaaaaag ggaaaggtct
     2521 gggttgcggc cgttcggcat tccttgacag tgcggattac gcgcggcgaa aatgcgggca
     2581 gcgcgttg
//
LOCUS       DATMGX010000400         3796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_270579, whole genome shotgun sequence.
ACCESSION   DATMGX010000400 DATMGX010000000
VERSION     DATMGX010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270579"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..419)
                     /locus_tag="VNR65_06450"
                     /pseudo
     CDS             complement(1..419)
                     /locus_tag="VNR65_06450"
                     /inference="COORDINATES: protein motif:HMM:NF022783.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
     gene            613..1059
                     /locus_tag="VNR65_06455"
     CDS             613..1059
                     /locus_tag="VNR65_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18351.1"
                     /translation="MLNIDELLPTAKEIRKQSALKEAEKADEHMRLMAAAEAEKQALV
                     NELSKPSGLSEDEKVKRASTVIQRAVRNGLHEVQVYRFPNILCTDRGRAINQMEPGWE
                     NTLTGTPKEIFHLWKDYLQPRGYKIAYQIIDFPGGVPGDIGITLSW"
     gene            1059..1955
                     /gene="ppk2"
                     /locus_tag="VNR65_06460"
     CDS             1059..1955
                     /gene="ppk2"
                     /locus_tag="VNR65_06460"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HWJ18352.1"
                     /translation="MRPARLAFRVIGQDDVYSSPIRATEQVNRGNTMAKDEPDSRMKR
                     KPYEKELRKLQVELCHLQRWVTTKGLRVVIVFEGRDAAGKGGTIRALTEKVSPRVFRL
                     VALPAPSDREKTQMFFQRYMQHFPAAGEIVIFDRSWYNRAGVEYVMGFCTKQEHERFL
                     ELCPETEKYIVDGGIILIKLWLEAGMEEQEKRFAARIKDPLRQWKLSPMDLESYRRWY
                     DYSKARDMMFKASDSKHAPWYVVRSEDKKRARLNCISHLLKTIPYRRLSPEQVKLPKR
                     SDKNRYNDLASLRRMKFVPERY"
     gene            1987..2169
                     /locus_tag="VNR65_06465"
     CDS             1987..2169
                     /locus_tag="VNR65_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18353.1"
                     /translation="MAEINKLSVEKVLNKLRGNDASNSSRIEQLDEKIKTADEEIQRL
                     RAARARLKRGQPAKRD"
     gene            complement(2230..>3796)
                     /locus_tag="VNR65_06470"
     CDS             complement(2230..>3796)
                     /locus_tag="VNR65_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HWJ18354.1"
                     /translation="IPVSLAYAGLAGLPPQAGIYGYLLGGLGYALLGSSRQLAIGPTS
                     AIALMMAGTVGAMAEGDVQRYAQIASLAAFTVAALSLVAWLLRLSVLVKLISDSVLVG
                     FKAGAGLTIAMTQLPSLFGVKGGGHNFFERAFTLAGQLGQTQYLVLVVGVVAILLLLL
                     GDRLFPGRPVALGVVALSIAAATLLGLPALGVPTTGEIPAGLPSLAGPALRLRDVEGI
                     IPLAAGCLLLAYIEGVSAARAFAAKHGYPLDARQELLGIGAANLAAAMGQGFPVAGGL
                     SQSAVNDKAGARTPLALVFASITLALCLLFLTGLLESLPKAVLAAVVLTAVYGLLDFP
                     AMRHMWRVSRLDFLAATVALVGVLLLGILQGILLAALASILLLLARVSRPHVAFLGRV
                     PGTNSYSDFDRHPENERLPGVIAFRPEASLIYANADAVLECVLNRLRDATPPAIRLTV
                     CDLSAAPFLDLAGSRMLRELNTELAGRGIALRIVGAHGSTRDLLRAEGLEEKVGVLDR
                     TATLDSVLRAGVG"
BASE COUNT          925 a         1132 c         1098 g          641 t
ORIGIN      
        1 cccggcattg gatcgggcat gaagccgaca aaatagcagg catggcctgc cttcatggca
       61 acgccgactt cgctgtcggc tttgaagccc ccaataccag ggccatgccc ggcacgagga
      121 tccaccacca caaatggccg acgcacagga ttgatttcga cgccttccgg tggaacgatg
      181 cgcaccagta ggtaattgac cggccgctac agcgtccgcc cgtccatcac cagctcgacc
      241 ttatagtcga ggacgtgggg gactttctcg gcgagatggt cgcgatattg gttgctgcgc
      301 tgacgcatca catcgcagaa caagacgccg cgttgcgtcg catctatcat gtattcgaag
      361 gcgggaccga aaagaccgaa gagcgtgttg cccgataatt gctgactgtc tgtcgacatt
      421 tttcagctcc atcttttcta ggcttctaac ttccctctcg ttattcaatg ctgcgcgctg
      481 ttaggttgat ctaacgcaaa ctgaatgctg caaaaacgag ctattttgcg ttcaaataaa
      541 tcggtacctc ggtacgtcaa aaacggcaga gatgcagacg tagagtgaaa aaaacgttca
      601 ggagcttcaa tcatgttgaa catcgatgaa ctgctgccca ccgcaaagga aattcgaaaa
      661 cagtccgcgc tcaaagaggc cgaaaaggcc gacgaacaca tgcgcctgat ggccgcagcc
      721 gaagccgaaa agcaggcgtt ggtcaacgaa ctcagcaaac cgtcgggcct ttcagaagac
      781 gagaaggtca aacgcgcttc gacggtcatc cagcgcgcgg tcagaaatgg gcttcacgag
      841 gtccaggttt acagattccc caacattctc tgtacggacc gcggccgggc gatcaaccaa
      901 atggaacccg gctgggaaaa cacgcttacg gggacgccca aggagatctt tcacctttgg
      961 aaggattacc tgcagccgcg cggttacaag atcgcctatc agatcatcga tttccccggt
     1021 ggtgttcccg gcgacatcgg catcactctg agctggtaat gaggcctgct cgcctcgcct
     1081 ttcgcgtgat cgggcaggac gatgtctact cttcacctat tcgagcgact gagcaggtta
     1141 accggggcaa cacgatggca aaagacgaac cggattcaag gatgaagcgc aaaccctatg
     1201 aaaaggagct gcgcaaactg caggtcgaac tttgccatct ccagcgatgg gtcactacca
     1261 aagggctgcg ggtcgtcatc gtgttcgaag gacgcgatgc cgcgggcaaa ggcggtacga
     1321 taagggctct caccgagaaa gtgagcccgc gggtatttcg gctggtggcg ctgcccgccc
     1381 cttccgatcg ggaaaaaacc caaatgtttt tccagcggta catgcaacat tttccggccg
     1441 cgggagaaat cgtcatcttc gaccgcagct ggtacaaccg cgctggcgtc gaatacgtca
     1501 tgggcttctg caccaagcag gagcacgagc gtttccttga gctttgcccg gaaactgaaa
     1561 aatatatcgt cgacggcggt atcatcctga tcaagctctg gctggaagcc ggcatggagg
     1621 agcaggagaa gcgtttcgcg gcccggatca aagatccgct gcggcaatgg aaactgagcc
     1681 cgatggatct cgaatcctat cggcgctggt acgactattc aaaagcgcgc gatatgatgt
     1741 tcaaggcctc cgattcgaag catgcgccgt ggtacgtcgt tcgctccgag gacaaaaagc
     1801 gtgcgcggct gaactgcatc tcgcacttgc tgaagacgat cccctacagg cgcctgtcgc
     1861 cggaacaggt caaattgccg aagagatccg acaagaaccg gtacaacgac ctggcgagct
     1921 tacggcgaat gaaattcgtg cctgaacggt actaggaagt cgagattcaa cgcggaggag
     1981 tttctgatgg ctgaaatcaa caaactctcc gtcgaaaaag tgctcaataa actgcgcggc
     2041 aacgatgcgt ccaacagctc gcggattgag cagctcgacg agaaaatcaa gacggccgac
     2101 gaagagatac agcgattgag ggcagcaagg gcgcgcctaa agcgcggcca accagcgaag
     2161 cgcgattgag agatccgtaa acctttctcg attgcgttgc ggggtccatc gacttcccgg
     2221 agaacactct taccccacac cggcgcgcag cacgctgtcg agcgttgcgg tacggtcgag
     2281 cacaccgact ttttcctcaa ggccctcggc tcgtagtaaa tcgcgcgtcg atccatgggc
     2341 gcccacgatg cgcagagcga taccgcggcc cgcaagttcg gtattcagct cgcgcagcat
     2401 tcgtgagccg gcaaggtcaa gaaagggagc ggcggagaga tcgcagacgg tcagacgaat
     2461 ggccggagga gtggcatcgc gaaggcgatt taggacgcac tccagcacag catcagcatt
     2521 ggcgtaaatc agcgatgctt cggggcgaaa agcgatcacg cccggcagcc gctcgttctc
     2581 gggatgccgg tcaaagtcgg aatagctgtt agtgccgggc acgcggccaa gaaaagcgac
     2641 atgtgggcgc gacacccgcg cgagcagcaa caatatcgag gccagtgcgg caagcagaat
     2701 gccctgaagt attccaagaa ggagcacgcc gacgagtgcg acggtcgccg ccaggaaatc
     2761 cagccggctc actcgccaca tgtgacgcat tgccggaaaa tccagcaagc cgtacaccgc
     2821 cgtcaacact acggcggcca gcacagcctt cggaaggctc tcaagcaagc cggtcagaaa
     2881 cagcaggcaa agcgccagcg tgatcgaggc aaaaaccagc gccagcggcg tacgtgcgcc
     2941 agccttgtcg ttcactgccg attgcgaaag cccgccggcg actggaaacc cctgccccat
     3001 cgccgctgcg agattagccg caccaatgcc taagagctcc tgcctggcat ccagcgggta
     3061 tccatgcttc gcagcaaagg cgcgagccgc ggacacgccc tcgatatagg ccagcagcag
     3121 acaacccgca gccagcggaa tgataccctc aacgtcacgc aacctcagcg ccgggcctgc
     3181 cagggaaggc aagcccgcgg gtatttctcc cgtcgttggc accccgagcg ccgggagtcc
     3241 caacagcgtc gcggcggcga tcgacaacgc gacaacgccg agcgcgaccg gccttcccgg
     3301 aaacagccgg tcgccaagta acagcagcag gatcgcgaca acccctacca cgagaacgag
     3361 gtattgcgtc tggccgagct gcccggcaag cgtgaacgcc cgttcgaaga aattgtggcc
     3421 cccgcccttc acgccgaaca ggctcggcag ttgagtcatg gcgatggtca gaccggcgcc
     3481 ggccttgaac cccaccagaa cgctatcact gatcagcttt accagcacgc tcaaccgcaa
     3541 aagccaggcg accaggctga gcgccgccac cgtgaaggcc gcgaggctgg cgatttgtgc
     3601 atagcgttga acatccccct cagccattgc tccgaccgtc ccggccatca tcagcgcgat
     3661 cgcggaagtg gggccgatgg cgagttgacg cgaggatccc aggagggcgt aaccaaggcc
     3721 gccaagcaaa tagccataga tgccggcttg tggcggtaag cccgcaagcc ccgcataggc
     3781 cagagaaacc ggaatc
//
LOCUS       DATMGX010000401         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_163488, whole genome shotgun sequence.
ACCESSION   DATMGX010000401 DATMGX010000000
VERSION     DATMGX010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163488"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VNR65_06475"
     CDS             <1..423
                     /locus_tag="VNR65_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18355.1"
                     /translation="SVLSRLRRATGIETALFESVTAEPGKVEPGSSRPTHVKAADLCD
                     RNQLHLVATRQTDAAARNELGSDSKNNIADCFLHLAALPTFALDRLSRYEHLLWQQAR
                     QLVFTLESLRRRKRQPSRSSFPFSFRRREPGALPEEFR"
     gene            954..1931
                     /locus_tag="VNR65_06480"
     CDS             954..1931
                     /locus_tag="VNR65_06480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWJ18356.1"
                     /translation="MAGARIVVVNFNAGENLHRCMAALLAQTKADFEIRIVDNASTDG
                     SLRYVPYDDRFLIIHAGGNVGFAAGCNLGARDCAAPLVIFLNPDAFPEPGWLEALLRA
                     AKSHPDAAMFGSLQVSATDGNMLDGAGDCYSCFGVPWRGGYGQPVKPLPDHAEAFSPC
                     AAAAMYRMEWFARVGGFDEKFFCYIEDIDLAFRVRLLGGRCMQVNSAIVRHVGSATSG
                     TTSDFTMFHSARNRFWTIIKNAPGWLLCVVAPLYIAFSAYLFFRFGRSSTASAIRKGV
                     FAGFAGLPGAWKQRREIQAARMASIGDIARAINWSISALRKREIVLRAW"
BASE COUNT          431 a          607 c          573 g          458 t
ORIGIN      
        1 agcgtcctgt cgcgactgcg tcgcgcgact ggcattgaga cagccctttt cgagtctgtg
       61 accgcagaac ctggcaaggt cgaacctggg tcttcacgcc cgacccacgt caaggcagct
      121 gatctctgtg atcgcaatca attacacctg gtagcaacac ggcaaactga cgcggcggcc
      181 agaaacgagc tcggctccga ttcaaaaaac aatattgccg actgtttcct gcacttggcc
      241 gctctgccga cttttgccct tgaccgcctc agccgctacg agcatttgct ctggcagcaa
      301 gcccgccagc ttgtgtttac gctggagtca ttacgccgcc gcaagcgaca gccgagccgt
      361 tcaagctttc cattttcgtt ccggcgtcgc gagccaggcg ccttgcccga agagttcagg
      421 tgaccactcg aaaggtcaac gcaaggcgcg ccgatcactg ctggggcgca ccaccagcgc
      481 gggacctaaa tcgaaaccaa tttgttccaa ttgcaaggat ggcttgcgat tagacaaagg
      541 attcggagcg cgagccgcat cttttaccct cggaagccga ttcaaactca cccaaggtaa
      601 ttttcgccaa attaggagag cccgacgcac ttcgtcgtgt cttcacaacg tcggctcctt
      661 atgcggcagc cgcacagcgg aacgacacat caaacatcac cgcctgcacg ggtcggcaaa
      721 atggccgact attcgcgctc aacgggattt gcatcgagga tagttccgcg ctgcttcgca
      781 gccttcaatt ccgtagatca gcctgcgccg cggcccagcg ccataccgtt tccacgaaat
      841 cctgacaacc accaaaccgt cctggtcgcc acgcaaacgc aaacctattg gctagccccg
      901 atagcacatc cctacaattt gcctgtatgg ccctatggca atacagttcg taaatggctg
      961 gggcgcggat cgtcgtcgtc aatttcaatg cgggcgagaa tctgcaccgc tgcatggcgg
     1021 ctttgttggc gcagactaaa gccgatttcg agatcaggat cgttgacaac gcatcgacgg
     1081 atggctcgct tcgatatgtc ccctacgacg accggttctt aatcattcat gccgggggta
     1141 atgtcggctt tgccgccggc tgcaatttag gcgcaagaga ctgcgccgca ccattagtaa
     1201 ttttcctaaa ccccgatgcc tttccggagc ctggctggct tgaggcgctt cttcgcgccg
     1261 cgaagagcca tcccgatgct gcgatgttcg gatcgcttca ggtcagcgcg actgacggca
     1321 acatgctcga cggtgccggc gattgctact cgtgttttgg ggtcccgtgg cggggcggat
     1381 acggccagcc ggtgaaaccc ttgcctgatc acgccgaagc tttcagccca tgcgcggcgg
     1441 ctgcgatgta tcgaatggaa tggtttgcgc gcgtcggtgg tttcgatgaa aagttctttt
     1501 gctacatcga ggacatcgat cttgcgtttc gcgtcagact cctcggcggc cgctgcatgc
     1561 aggtaaattc cgcgatcgtg cgtcatgtcg gcagtgcgac atcgggaact accagcgatt
     1621 tcaccatgtt ccacagcgct cgcaaccggt tctggaccat catcaaaaac gcgcccggct
     1681 ggctgctttg cgtcgtggca ccactttata tcgcgtttag cgcttatttg ttctttcggt
     1741 tcgggaggtc atcgacagcg tcagcgataa ggaaaggcgt gttcgcgggc tttgccggtc
     1801 tgccgggtgc gtggaagcag cggagggaaa ttcaggccgc cagaatggcc tctattggcg
     1861 atatcgcgcg cgcaataaat tggtcaattt cagcattgcg caagagagag atcgtcttac
     1921 gcgcgtggta gaagcgcgta ggtgcaacaa gaaacgccgg cacgtgttcc tggcgcgcgc
     1981 tcttgctcct tatatcaacc cggcgaaaat cgcaggattt ttctatgcgc aatcgctcta
     2041 tttgagttac gggcgggaaa agattgaag
//
LOCUS       DATMGX010000402         4285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_428195, whole genome shotgun sequence.
ACCESSION   DATMGX010000402 DATMGX010000000
VERSION     DATMGX010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428195"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..2296)
                     /locus_tag="VNR65_06485"
     CDS             complement(194..2296)
                     /locus_tag="VNR65_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWJ18357.1"
                     /translation="MNQKVQIMPSEPPAAEKRPVRDVHHGIERVDDYAWLRVPNWQEV
                     MRDPQALSADVRGYLEAENAYTKEVMADTEALQETLFKEMKGRIKEDDSTVPAPDGAY
                     AYFMSYVMGGQHPRLCREPRAGGEMQVLLDGDALAAGKPYFSLGAATWSRDHSLLAYA
                     FDDKGSEFYRIRVRHLETGKELPDEVPDMEGQPVWNADGSGFYYTRVDEHRRPQQVLL
                     HRICHKADEDELIYREPDAGFFTRVGHSQYRRYLFISAGDHETSEIRFLDLSAPQKGS
                     RLIATRETNHDYQAEHHEGRLIILTNSDGAEDYKIVEAPIENPGRENWSDLVPHKPGR
                     LILDYTCYKDYLVRLEREDGLPRIVIRRFSDGEEHAIDFAEEAYSLGMSDGYEYDTET
                     LRFTYSSMTTPRQVFDYNMRTRERVLRKTQEVPSGHNPADYVTRRVFAPAWDGEKVPV
                     SLLYRKDTPLDGSAPCLLYGYGSYGIAIPAGFSTNAFSLVDRGFVYAIAHIRGGKDKG
                     YRWYKDGKLAKKTNTFKDFISAGRYLAEQKFTSAGRIVGQGGSAGGMLMGAVANMAPE
                     LFAGLIADVPFVDVLTTMLDDTLPLTPPEWPEWGNPIESADDYRTIAAYSPYDNVRAQ
                     AYPNILATGGLTDPRVTYWEPSKWVAKLRAHKTDKNLLLLKINMDAGHGGASGRFDRL
                     KEDALADAFALKVAGKAS"
     gene            2549..2638
                     /locus_tag="VNR65_06490"
     tRNA            2549..2638
                     /locus_tag="VNR65_06490"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2583..2585,aa:Ser,seq:cga)
     gene            complement(2727..3053)
                     /locus_tag="VNR65_06495"
     CDS             complement(2727..3053)
                     /locus_tag="VNR65_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18358.1"
                     /translation="MDTEKYLKVAVLAALLIIALVYPVVSALVLTGVAAWHLQNFKIG
                     DMALVQKVVQLTPPGIKATVTIRTASPLPSADAPVKLALPPPDALRDAVLMVDTRVRE
                     AMIDHR"
     gene            3430..4083
                     /locus_tag="VNR65_06500"
     CDS             3430..4083
                     /locus_tag="VNR65_06500"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWJ18359.1"
                     /translation="MADNPFEIPQTLRNLSEQNLKQAHAAYEQLSGFVTKAMDAWMGA
                     MPANPMTAGFKDVQGRAMEIAMENAESAFTFAGMICNAPTPQDIMTLQTQFAQERMQA
                     FVTQTQELFSFMGEALPKSERSAMDVSMGAMPSNLIPSNLMTAGIRSVQDRAVAMAKQ
                     NAESAVALAEKIGKAQNVQDIFGLQARFALEQMKAYAGQTQELQKLIGAALQKPERG"
BASE COUNT          878 a         1259 c         1272 g          876 t
ORIGIN      
        1 aggagcttga atcagaattc gcaattcctg ggaatcctga atctcaacag gccctagtat
       61 aaatcattga tgttaaaagc tattcgtcgc aggtggctgg cgccggggtc cgtgcgccgc
      121 cgtaagcagc ggggccatcg aatttccgca tggctgcaag cagcgatcca atggcgcccc
      181 cgataaacgc tccttagctc gccttccccg caaccttcag cgcgaaggca tcggcgagcg
      241 cgtcttcctt cagccggtcg aaccggcccg acgcgccacc atggcctgcg tccatgttga
      301 tcttcaagag cagcaggttc ttgtccgtct tatgcgcgcg gagcttcgcc acccatttcg
      361 atggctccca ataggtgacg cgcggatcgg tgaggccgcc tgtcgcgagg atgttcggat
      421 aggcttgcgc gcgcacattg tcgtaaggcg aatacgccgc gatggtgcgg taatcgtcgg
      481 cgctttcgat ggggttgccc cattcgggcc attccggcgg ggtgagtggc agcgtgtcgt
      541 cgagcatggt cgtcagcacg tccacgaacg gcacgtccgc gatgagcccc gcgaacagct
      601 ccggcgccat gttcgcgacc gcgcccatga gcatgccgcc tgccgagccg ccctggccca
      661 cgatcctgcc cgcgctagtg aatttctgct ccgcgaggta acggcctgct gagatgaagt
      721 ccttgaaggt gttcgtcttc ttggcgagct tgccgtcctt gtaccagcga tagcccttgt
      781 ccttgccgcc gcgaatgtgg gcgatggcat agacgaaacc gcgatccact aggctgaagg
      841 cgttggtcga gaagcccgcc ggaatggcga tcccgtacga gccgtagccg taaagcaggc
      901 aaggcgcgga gccgtcgagc ggcgtgtcct ttcggtagag cagggaaacc ggcaccttct
      961 cgccgtccca agcgggcgcg aaaaccctgc gcgtgacgta atcagctggg ttgtgaccac
     1021 ttggcacttc ctgcgtcttg cgcagcaccc gctcgcgggt gcgcatattg tagtcgaaca
     1081 cctgccgcgg cgtggtcatc gacgagtaag tgaagcgcag ggtctcggtg tcgtattcgt
     1141 agccgtccga catgccgagc gagtatgcct cttccgcaaa gtcgatggcg tgctcctctc
     1201 cgtccgagaa gcggcggatc acgatgcgcg gtaggccgtc ctcgcgctcg aggcgcacca
     1261 gataatcctt gtagcaggtg taatcgagga tgagtcgccc cggcttatgc ggaacaagat
     1321 cgctccagtt ttcgcggcca ggattttcga tgggcgcttc tacgatctta taatcctcag
     1381 cgccatcgct gttggtgagg atgatgagcc gcccctcgtg atgctccgcc tgataatcgt
     1441 ggttcgtttc gcgcgtcgca atgagccgcg agcccttttg cggcgcagac agatcgagga
     1501 agcgaatctc gctcgtttcg tgatcgcccg cgctgatgaa cagatagcgc ctgtactggc
     1561 tgtgtcccac ccgcgtgaaa aacccggcgt cgggctcgcg gtagatcaac tcatcctcat
     1621 ccgccttatg gcaaatgcgg tgcagcagca cttgctgcgg cctgcgatgc tcgtcgacgc
     1681 gagtgtaata gaagccgctt ccgtcggcat tccagacggg ctggccttcc atatcgggaa
     1741 cttcgtccgg caattccttg cccgtctcaa ggtggcgaac cctgatcctg tagaattcgg
     1801 aacccttgtc gtcgaaggcg taagccagga ggctgtgatc gcggctccag gtggctgcgc
     1861 cgagcgagaa atagggcttg cccgcggcca gcgcgtcgcc gtcaagcagc acctgcatct
     1921 cgccgcccgc acgcggctcg cggcaaaggc gcggatgttg accgcccatg acatagctca
     1981 tgaaataagc ataagcgcca tccggcgctg ggactgtgga atcgtcttcc ttgatgcgcc
     2041 ccttcatctc cttgaacagc gtctcctgga gcgcttcggt gtcggccatg acctctttcg
     2101 tgtaggcgtt ttccgcctcg agatagccgc gcacatcggc gctgagcgct tgcgggtcgc
     2161 gcatcacctc ctgccagttc gggacgcgca accaggcata atcgtcgacg cgttcgatgc
     2221 cgtgatgaac atcgcggaca gggcgctttt cggctgcggg cggctcggaa ggcatgattt
     2281 gcactttctg gttcatgggt tgccattcat tgccggattt gagccttgtt gtaggcattc
     2341 gatgagcgca tctcaaccgc tgatcgaaaa agcggcaggg gatgtcagga aataattatc
     2401 ccgttggcct tggttgagtg ctcaaaaaag cgacttgcgg gccgttgaag ccgttgcgcg
     2461 cctgccgcgc gtttgctatc tgttcgcacg gacctgcaaa aagggagccg gcgattaata
     2521 ggctgccttt gtgcgcggtg catcccttgg agaggtgcta gagtggttga ataggccggt
     2581 ctcgaaaacc ggagtacggg taaccgtacc gagggttcga atccctccct ctccgccaag
     2641 cggtccaata atacactgtt tttaaagcga aatgcccatg cgtcagccca tggttgatgg
     2701 gtataaacca gggcacaata gcaaatttag cgatgatcta tcatggcttc acgtactctc
     2761 gtgtcaacca tcagcaccgc atcgcgaaga gcatcgggcg ggggtaaggc aagcttcacc
     2821 ggtgcatctg cggaaggcag ggggctggcg gtacggatcg tcacggtcgc tttgatgccc
     2881 ggaggggtga gctgaaccac tttttgcacg agtgccatgt cgccgatctt aaaattctga
     2941 aggtgccacg cggcgacacc cgtaagaaca agcgccgaaa cgacagggta caccaacgcg
     3001 atgatcaaga gcgcggccag aaccgcgacc ttcaagtatt tttcggtatc catttgactt
     3061 tgtctccaag gcgcggtgtc gcgatgataa tcgtagcagc atcccgaagt gcgctaagcc
     3121 gattctccta aatgaaatct aaaaacgcct aaataggacg aggaattcgt cggtcgatca
     3181 agagctactc gggcagccat gaaaaggaaa cggcgcccga aggcgccgcc catagcccgt
     3241 tgtgaggacg gtctacttcg gaggctcgtg tccgcgtcgc cgaatcgccg cctctccctc
     3301 gaaaatccga gacgccggcg cgctcggcac agccggtccg gttcgccata ttctcaatat
     3361 ctcgggacac gatctcggga cacggctaca cttaattctt cctcaacgca gcccctggga
     3421 ggggacgcca tggccgacaa cccattcgaa atcccgcaga ccctgcgcaa tctgtccgaa
     3481 cagaacctga agcaggccca tgccgcctat gagcagctta gcggtttcgt gaccaaagcc
     3541 atggacgcct ggatgggtgc gatgcccgca aatcccatga ccgctggctt caaggatgtg
     3601 caaggccgcg ccatggagat cgcgatggaa aatgccgagt cagcttttac gttcgccggc
     3661 atgatctgca acgcaccgac cccccaagat attatgacgc ttcagacgca gtttgctcaa
     3721 gagcggatgc aagccttcgt cacgcagacg caggaacttt tcagcttcat gggagaagct
     3781 cttccgaagt cagaacgcag cgccatggac gtctcgatgg gggcgatgcc ctcaaatctg
     3841 atcccatcaa acctcatgac cgctggcatt agaagtgtac aagaccgtgc cgtagccatg
     3901 gcgaagcaga atgccgagtc ggcagtagct ttggccgaaa aaatcggcaa ggcacagaat
     3961 gtccaggata tttttggact tcaggcccgt tttgctctgg agcagatgaa ggcttacgcc
     4021 ggacagacgc aggaacttca aaagctaatc ggggcagctc tccagaagcc agaacgcggc
     4081 tgattctcta agaagaaact cacccggccg caacgcggct gggcggccgg agcaggcgca
     4141 gccactggtt ttttgaatgc catcaagcgg cctccgctag aggttgaagt tcgaccttga
     4201 aaccaagctt ggcgagttga gcaaccaggc gattcgcttt gattacggct ggacggcggt
     4261 cgaaatggcc ggcccccaga tcgtt
//
LOCUS       DATMGX010000403         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_9538,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000403 DATMGX010000000
VERSION     DATMGX010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9538"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..1709
                     /locus_tag="VNR65_06505"
                     /pseudo
     CDS             328..1709
                     /locus_tag="VNR65_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611236.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1964..2179)
                     /locus_tag="VNR65_06510"
     CDS             complement(1964..2179)
                     /locus_tag="VNR65_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ18360.1"
                     /translation="MTKTQVEVITSVQRRRRWSRVEKERIVAAALEPGAVASEVAREA
                     GIHTSQLFRWRQQLCKRTPAPVAGTCQ"
BASE COUNT          448 a          764 c          656 g          407 t
ORIGIN      
        1 ttttttgagc ggaccggcga ttgtctgact gccttttgga gccgttggac acatgtcgga
       61 cactgagctt ttgcttaaat cggagccgcc acgacggctg gaagtattta ccggtgctgg
      121 tcggcgccgc agttggacgc ctgagcagaa ggcgcagatc gttgccgagt gccccccgtg
      181 ccaatgtgag gtgagacacc tgtctcgggc aatttagagt acaggtagga catgaacatg
      241 acctcattgc tgaaatcttc tctgaatgga ttgtcgggtt cttcctccga ggctgtcgcg
      301 gaaggagccg gtagggcaac tggagagatg ggctcgggcg aaggcgcgcg ccgtgacccg
      361 gaggtcgttg cgatcgccag gcgccgccag ttctccggga gcgagaagcg ccggttgctc
      421 gccgaggccg agcgctgcaa ggatgtcggt acgctcggcg cattcctacg ccgcgagcac
      481 atctactcct cgatgatctc gggctggcgc aagcagatcg gcagggcaaa tcaggcagcg
      541 ctcgctccaa aaagacgggg gccaaagccg gatgcctcgg cacgacagat ccagcagctg
      601 gagcgcgaca acgctcgtct gcgccacaag ctcgaacgcg ctgagctgat catcgacgcc
      661 caaaaaaaac tgtgcgtggc cctggggctg ccgacggcgg acgacaggag cggggatgcg
      721 tgatgctcgc cgtggccgag ctcgctccac gcgtcggcct gaggagtgcc tgtcaggctt
      781 tcgcgctaaa ccgcgggttc gtgtaccgcg atcgtgcccg gcgcagcgtg accgcgtcac
      841 gccgcgcacc gcagatgcgg ccacggcccc ccttggcgct ctcgagcgcc gagcaggatc
      901 tattgctggg acttctcgac agcgagcgct ttgcggacgt ggcgccggcc acggtcttcg
      961 ccacgttgct cgatgaaggc cgctatcacg gctcgatccg taccatgtat cggctcctgg
     1021 cagcccagca tcaaactggc gagcgccgcc gtcagcgaat tcatccgctc tacgccaagc
     1081 ccgagctcct ggcgatccgg cccaacgaag tgtggtcctg ggatatcacc aaattgaagg
     1141 gcccggccaa atggacctgc ttccatctct acgtgatcct cgacatttac agccgctacg
     1201 ttgtcggttg gctgctcgct catcgagagt ccgcagaact cgccgagcag ctcatcgccg
     1261 acaccttgga aaaggaaaac atcgcccccg ggacccttac gctccatgcc gatcgcggta
     1321 ccagcatgcg atcgaagccg gtcgccgcgc tcctcatcga cctcgatgtt gccaagaccc
     1381 acagccgccc gcatgtctcg gatgacaatc catactccga ggcgcaattc aaaaccctga
     1441 agtacagacc tgacttcccc gatcgtttcg gctcgatcga ggacgcccgt gctcactgcc
     1501 ggcacttctt ccactggtac aatcacgaac atcgccacgg cggtatcggc ttgatggctc
     1561 cggccgccgt ccacgacggc accgctgcgg cgctcaccga gcaacgcgcc atcaccctcg
     1621 atgccgcgtt cgctgcacat cccatccgct tcaagggcct cgccccaaag ccgcccgcac
     1681 tgcccgccgc cgcttggatc aacccaccaa aaaaggaggc gatgcccgtg ccgcgcgaca
     1741 atcaaaaacc cacgttcagc gacaatccgc cgatgcatct catggtggaa cacgcttcgc
     1801 tcggcgctac gccagtcaag cccgaagccg ccagaggcgg tggcctgaaa ggccaggctt
     1861 tacgggcgta tcgctgagcg agatacttac aaatgagatg caacccaaac tcctcaatca
     1921 aacccgcttg ctcgctaatc tcatgacaaa aggtgtttca aagtcattga cacgttccgg
     1981 cgacgggcgc cggcgtccgc ttgcagagct gttgccgcca ccggaatagc tggctcgtgt
     2041 gtatcccggc ttcgcgcgca acctccgagg ccaccgcacc cggctccagc gccgccgcaa
     2101 cgatccgctc cttctccaca cgagaccatc gccggcgtcg ttgcaccgat gtgatcacct
     2161 caacctgcgt cttggtcata gggctaatcc taggattagt cctaggactt caccgaaacc
     2221 tcagcccccc gcaaggcggc cgtcgccggg tggataccaa taccaagcac tcttc
//
LOCUS       DATMGX010000404         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_173484, whole genome shotgun sequence.
ACCESSION   DATMGX010000404 DATMGX010000000
VERSION     DATMGX010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173484"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VNR65_06515"
     CDS             complement(<1..418)
                     /locus_tag="VNR65_06515"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18361.1"
                     /translation="MIPLATREIHDQKSVERPPQASRIGIIAGGGSLPLAVAEAARAS
                     GYSPFIIGLTENASTDIERFPHAYAGIGQVGFTLRTLRREGCQRIVLVGSLRRPNLFR
                     LRLDTGFFRHLPQLLRLFKGGDDAVLRRVARFFEAQG"
     gene            complement(415..1212)
                     /gene="lpxA"
                     /locus_tag="VNR65_06520"
     CDS             complement(415..1212)
                     /gene="lpxA"
                     /locus_tag="VNR65_06520"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWJ18362.1"
                     /translation="MTEMMVHPAAAIDRAAKLEEGVEVGPFAVIGPNVTLRKNVKIHS
                     HAVVTGRTEIGEGCEIHPFAVIGGAPQDMKYRGERSELVVGPFNTIREHVTMNGGTEG
                     GGHVTRIGSHGLFLAGAHVAHDCQIGDHVILVNNATLAGHVTVEDHAILGGLCAVHQF
                     VRVGAYAFVGGMSGVESDVIPFGMVLGNRASLAGLNIVGLKRQGFDREQIHTLRKAFG
                     LLFSQQGTLQERLDDVEKMFPGDAPVQRIVAFMRAKTDRSFCVPRHA"
     gene            complement(1221..1751)
                     /gene="fabZ"
                     /locus_tag="VNR65_06525"
     CDS             complement(1221..1751)
                     /gene="fabZ"
                     /locus_tag="VNR65_06525"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HWJ18363.1"
                     /translation="MRQISETNSEGEAQAEKKALGTADVVRILKLLPHRYPFLLVDKI
                     VDMDGDSSAVGIKNVTINEPFFQGHFPNFPVMPGVLLIEGMAQTAGALCMANLADSCE
                     PQLVYFMSIDRARFRRPVLPGDTVHYHMTKKRNRGRVWRFEGKAKVNGQLVAEAEISA
                     MIVDSGQGASRSAGNG"
     gene            complement(1763..2818)
                     /gene="lpxD"
                     /locus_tag="VNR65_06530"
     CDS             complement(1763..2818)
                     /gene="lpxD"
                     /locus_tag="VNR65_06530"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWJ18364.1"
                     /translation="MDHPGFWAPAGPFSLSELASYTGAEFPEVSMREAAKSICSVRTL
                     DDAGGSDVSFFDNRKYLSALQLTEAAAVFVSADYARRVPAACIPLVSRDPYRAFAKSL
                     EMFFPDAGHPKVGGGTIAIDESARIEDGAVIEAGAIIGPQAQIGRGSRIAAGAVIGYR
                     VTVGRDCFVGPGASVTHALIGNRVIIHAGARLGQDGFGFAMGPSGHYKVRQVGRVIVQ
                     DDVEIGANTTIDRGALKDTIIGEGTKIDNLVQIAHNVVIGRHCVIAAQTGISGSTILE
                     DFVAMGGQCGTVGHIRIGAGAQVGAQSGVHCDVPRGERWGGYPAKPIVSWAREVAILK
                     RLTRRKSTVESAGDSNE"
     gene            complement(2910..>3128)
                     /locus_tag="VNR65_06535"
     CDS             complement(2910..>3128)
                     /locus_tag="VNR65_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HWJ18365.1"
                     /translation="AYLNKHGGNCPAAGFNYQCNLVDDSDAIRASVGASLIWNSPIGP
                     LRADFGYAILKEAFDQTQVFRFGAATKF"
BASE COUNT          649 a          987 c          888 g          604 t
ORIGIN      
        1 acccttgcgc ctcgaaaaaa cgagcgacac gccggagcac cgcgtcgtcg cctcctttga
       61 aaaggcgcag aagctgcggg aggtgacgga aaaagccggt atcaagcctg agccgaaaaa
      121 ggttgggccg tcgcaggctc ccaaccagca cgatgcgctg gcacccctcc cggcgaagcg
      181 tacgaagagt aaatcccact tgcccgattc cggcatacgc atgaggaaag cgttcgatgt
      241 cggtggatgc gttttccgtg aggccaatga tgaacgggct atatccactt gctcgagccg
      301 cttcggcgac agccagtgga agagagccgc cccctgcaat gatcccgatc cgcgaggctt
      361 gcggcgggcg ttctacgctc ttttggtcat gaatctctcg cgtggcgagg ggtatcatgc
      421 gtggcgcgga acgcagaagg agcgatccgt tttcgcccgc atgaaagcga cgatgcgctg
      481 gaccggcgcg tcgccgggaa acatcttctc cacgtcgtcg agccgctcct gcaatgttcc
      541 ctgctgagaa aacaagagcc cgaaagcctt gcgcagcgta tggatttgct cgcgatcgaa
      601 gccctgccgc ttgaggccga cgatattgag gcccgcgagg cttgcgcgat tgccaagcac
      661 catgccgaac ggaatgacat cactctcgac gccagacatc ccgccgacga aggcataagc
      721 gccgacgcgc acgaactgat gcaccgcaca caacccgccc aggatggcgt gatcctcgac
      781 ggtgacatgc ccggcaagcg ttgcgttatt gacgaggatg acatgatcgc cgatctggca
      841 atcgtgcgcg acgtgggcgc cagcaaggaa cagtccgtga ctgccgatac gcgtcacatg
      901 gcccccgcct tcggtgccgc cattcatggt gacgtgctca cgaatggtgt tgaaagggcc
      961 gacgaccagc tcgctgcgtt cccccctgta tttcatgtcc tggggcgccc cgccgatgac
     1021 ggcgaagggg tggatttcgc aaccctcgcc gatctcggtg cgccccgtga ccacggcgtg
     1081 ggaatggatt tttacgtttt tgcgaagcgt gacattcgga ccgatcacgg cgaacggccc
     1141 aacttcaacg ccttcttcca gctttgccgc tcggtcaata gcggccgccg gatgcaccat
     1201 catctcggtc attgaatcgc ttatccgttt cctgcggacc ggctcgcccc ttgccccgaa
     1261 tcgacgatca ttgcgctgat ctcggcttcc gcgacgagtt ggccgttcac cttggcttta
     1321 ccctcgaaac gccagacacg cccgcgattg cgctttttgg tcatgtgata gtgcacggta
     1381 tccccaggca gaacggggcg gcggaagcgc gcgcggtcga tcgacatgaa gtaaacgagt
     1441 tgcggttcgc aggagtcggc gagattcgcc atgcagagcg cgcccgcggt ttgagccatc
     1501 ccctcgatca gaagcacgcc gggcatgacc gggaagttcg ggaaatgacc ctgaaagaaa
     1561 ggctcattga tcgtgacgtt cttgatacca accgccgaac tgtcgccgtc catgtcaacg
     1621 atcttgtcga cgagaagaaa cggatagcga tgcggcaaca gcttcaagat gcgcacgaca
     1681 tcggctgtgc cgagcgcctt tttctcagcc tgcgcctcac cttctgaatt ggtctcagat
     1741 atctgcctca tgaccccatt ttctattcat tcgaatcgcc ggcgctctca accgttgatt
     1801 tacgcctcgt cagacgcttc aaaatcgcaa cctctcgagc ccacgatact ataggcttgg
     1861 cgggataacc gccccaccgt tcgccgcgcg gcacatcaca gtgaaccccg ctctgagcgc
     1921 cgacctgggc ccctgcgcca atgcggatat gtccaaccgt tccacactgc ccgcccatag
     1981 ccacaaagtc ttccaggatc gtactgcctg atatgcctgt ttgcgcagcg atcacgcagt
     2041 gacggccgat aaccacgtta tgggcgattt gaacgaggtt gtcgatcttt gtgccctcgc
     2101 cgatgatggt atccttcagc gcacctcgat cgatcgtcgt gttggcgccg atctccacgt
     2161 cgtcctggac gatcacacgc ccgacctgcc gaactttgta atgtcctgac ggacccattg
     2221 caaagccgaa accatcctga cccagccgag ctcccgcgtg aataatgacg cggttgccga
     2281 tcaaagcatg cgtaacagat gctcccgggc ccacgaaaca atcccgcccc acggttacac
     2341 ggtatccgat gaccgccccg gctgcgatcc gcgagcctcg cccgatctgt gcctgggggc
     2401 cgatgatggc gccagcctcg atcacggcgc cgtcctcgat ccgggcactc tcgtcgatgg
     2461 ctatcgtgcc tccgccgact ttcggatgcc ccgcgtctgg aaagaacatc tccagcgatt
     2521 tggcgaatgc gcgataagga tcgcggctaa caagcggtat gcacgccgcg ggaacccgcc
     2581 tcgcatagtc agcggacacg aagacagcgg cggcttcagt aagctggagc gcgctcagat
     2641 atttccggtt gtcgaagaaa gagacatctg aaccgcccgc gtcgtccagc gttctgaccg
     2701 aacatatcga cttcgccgcc tcccgcattg agacttccgg gaactcggcg ccggtgtagg
     2761 acgcgagttc tgaaagagag aacggccccg ctggggccca aaagccagga tgatccatga
     2821 tgcgctgcac agccaaaaag ctaagcgggc ttctgcccgc ttagcacctc tcttaaaccg
     2881 aatgtgggac gccgacgacc tttggcttac taaaacttgg tcgcagcacc aaatctgaag
     2941 acctgcgttt gatcgaaagc ctctttcagg atggcatagc cgaagtccgc gcgtagcggc
     3001 ccgatcggag agttccagat caagctcgcg ccaactgagg cacggatggc gtcagagtcg
     3061 tccacaagat tgcactggta gttgaagccg gctgcggggc agttaccccc atgcttgttc
     3121 agataggc
//
LOCUS       DATMGX010000405         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_53221, whole genome shotgun sequence.
ACCESSION   DATMGX010000405 DATMGX010000000
VERSION     DATMGX010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53221"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VNR65_06540"
     CDS             <1..284
                     /locus_tag="VNR65_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18366.1"
                     /translation="QRAMVNDLVSKTLLAAREHDVATLLVTGGVAANSELRTTFEQRA
                     SEEGLAVYFPSRKLSTDNAAMIAAAAYPRFLAGEFAAPELSADAALRLR"
     gene            complement(375..1283)
                     /locus_tag="VNR65_06545"
     CDS             complement(375..1283)
                     /locus_tag="VNR65_06545"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HWJ18367.1"
                     /translation="MKKIVAAVLLIFSMTTWAWCQNRGAQVDRVQTASTVLSQIMSAP
                     DRAIPDGIMSGAKCIAIVPSMLKASFVFGANYGKGVATCRTEHGWSAPVFFKVTGGSF
                     GFQAGGQASDLVLIIRTDDGMQHLLQSKFKLGADASAAAGPVGRDAQAMTDLTLRAQV
                     LTYSRSRGLFLGVSLSGGVIRQDDGDTRAFYGGKDWTFYSLLNGQVPAPQDSMVFLNT
                     VEKYAPTPKVRAQATPAPVVTAPAIAATPVETPTTASPATPAEAPAATSDSTTDETPA
                     TAPAATPSSATPPVTATPASGGTVNP"
     gene            complement(1739..2392)
                     /locus_tag="VNR65_06550"
     CDS             complement(1739..2392)
                     /locus_tag="VNR65_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ18368.1"
                     /translation="MKPPPKSTIRIAVVESDPLRFLGFRAIFSSEEDFRIRAATVPSI
                     LDSPDDDIILMTSSGGAAFHAAMSALKAVRPTIRIIVTGAGSSDEEILRAIAAGAKGY
                     IAEGATPDEFKHAIQAVHSGSVWAPRRVLCTFIERATASPRRVLPQGEDKISEREREV
                     LKLLVAGRSNKEIGDELGIEVRTVKAHVSQLMRKVGVQNRIALSVHAITHSLLSSRP"
     gene            complement(2520..>2894)
                     /locus_tag="VNR65_06555"
     CDS             complement(2520..>2894)
                     /locus_tag="VNR65_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18369.1"
                     /translation="MYYSDEVRQLESFGPVDVELKDAEVKVAHQLIEALAGKFEPEKY
                     HDTFEENLKQLIKARLEGQEVAAVEKPRKLAPVVDLMAALKESLAQMPKKPPQRAAAS
                     QRESEVKVAKKSAKGSRRGSAA"
BASE COUNT          565 a          859 c          866 g          604 t
ORIGIN      
        1 tccagcgcgc gatggtgaac gacctggtca gcaaaacgct actggccgcg cgcgaacacg
       61 atgtcgcaac gttgctggtg acgggcggtg tcgccgccaa tagcgagttg cggacgacat
      121 tcgaacagcg cgcctccgaa gaaggtttgg cggtctattt cccctcccga aaactttcca
      181 cggacaatgc ggccatgatt gcagccgctg cttatccacg atttctggcc ggtgaatttg
      241 ccgcgccaga actatctgcc gacgcggccc tgcgcctgcg ttagtcgagc aaagcaactc
      301 caaagtggtt ataccctacc cgtacaggcg gatacactaa tcctgaaacc accctaatta
      361 atccgtcgct gctcttaagg attgaccgtt ccgccgctag ccggtgtagc tgttaccggc
      421 ggcgtggccg aacttggcgt tgcggccggg gcggtagccg gcgtttcgtc ggtcgtcgaa
      481 tccgatgtcg ccgccggcgc ttcagcagga gttgccggtg aggctgtcgt cggtgtctcg
      541 actggggtgg ccgcaatcgc cggcgcggtt acgacggggg caggtgtagc ctgcgcgcga
      601 accttcggcg tgggagcgta cttctcaacc gtgttcagga aaaccatcga gtcctgcggg
      661 gccggaacct gtccgttgag caatgagtag aatgtccagt ccttgccacc atagaaggcg
      721 cgagtgtcgc cgtcgtcctg gcgaatgaca ccgccgctca acgagacacc gagaaacaat
      781 ccgcgcgaac gcgaataggt cagcacctgg gcgcgcaacg taaggtcagt catggcctga
      841 gcatcgcggc ccaccggccc ggctgccgcg gaagcgtccg ctcccaactt gaacttgctt
      901 tgcagcaggt gctgcatgcc gtcatcggtg cggatgatca ggaccagatc ggaagcctga
      961 ccgcccgcct ggaatccgaa actgccgccc gtcactttga aaaacaccgg cgcgctccag
     1021 ccatgctcgg tgcggcaagt cgctacgccc ttgccatagt tggcgccgaa gacgaaactc
     1081 gccttcagca tagagggcac aatcgcgatg cactttgccc ccgacatgat tccgtcggga
     1141 atggccctat cgggagcaga catgatctgg ctgagcacgg tgctcgccgt ctggacgcga
     1201 tccacttgcg cgcctcggtt ttggcaccaa gcccaggtgg tcatcgaaaa gatcaacaac
     1261 actgccgcga caatcttctt catggctcga ctccgatcaa gaacggtgtg ctccgccagc
     1321 cggtggcggc tgcacccatc ttacgagggt tggatggaat agcgcgccgg aagaaagcgc
     1381 aaggctggtt actctttagt gacctgacgt cgatgggtga gaacgggagc ggagccagtc
     1441 cggatggttt ttgccagttc agatgggggt tgattccaaa aacgaagggc tggaagagga
     1501 gaccctcgta acctcttcca accctatctc ccaggttctg tggtaggtga atgcagtatg
     1561 cagtcgtcgc tggattcggt caagtaacag aaggtcaggc ctggtaactg tactttcgta
     1621 acattcagag gggcaggtaa cttggcccct gaactggatt gttccacgtg aaacaagttg
     1681 gcgggattgc tcgtttgaat gaccgagccg cgctttgtcc ggcggtcggc gaactggatt
     1741 acggccgcga cgagagcagc gagtgcgtga tggcgtggac tgacaaggcg atgcggttct
     1801 gcactccgac cttgcgcatg agctgactga catgcgcctt aacggtgcgc acttcgatac
     1861 cgagctcgtc tccgatctct ttgttggagc ggcccgcgac cagcaacttc agcacttcgc
     1921 gctcccgttc gctgatcttg tcttcccctt ggggtagcac cctgcgaggc gatgcggtag
     1981 cgcgttcgat aaacgtgcac agcactcgcc taggcgccca caccgaaccg ctgtgcacgg
     2041 cctggatcgc atgtttgaat tcgtcgggcg tcgcgccctc ggcaatatag cccttcgcgc
     2101 ctgctgcaat cgcccgcaga atctcttcat cgctggagcc ggcccccgtg acaataatgc
     2161 gaatggtggg acgcactgcc tttagcgcgg acatcgccgc gtggaatgcg gccccgccac
     2221 tggaggtcat caggatgatg tcgtcgtcag gagaatctaa gatcgagggg acggtggcag
     2281 cgcgtatccg aaagtcctcc tcggaggaga atatcgcccg aaatccaagg aagcgaagcg
     2341 gatcgctctc caccacggca atgcggatgg tgctcttagg cggcggtttc attcggagac
     2401 tcaaggtgcc cgtagaatac cgcattacaa ccttggtgca tatgggaatg gcacgacagt
     2461 gctattggcg gcaggggtca ctcgaaacct gccgccaatg acgatgccgg ttgacagcat
     2521 catgcagcgg agcctcggcg tgaacccttg gccgacttct tcgccacttt gacctccgac
     2581 tccctttggg atgccgctgc gcgttgcggg ggcttcttcg gcatctgtgc caggctctcc
     2641 ttgagtgccg ccataaggtc aaccaccgga gcgagcttgc gaggtttctc cacggcagct
     2701 acctcttgcc cttccaaccg cgctttgatt agctgcttca ggttttcctc gaaagtgtcg
     2761 tgatatttct cgggctcaaa ttttccggcg agcgcttcga tcaactgatg ggcgaccttg
     2821 acctcggcgt ctttcagttc gacgtcgacc gggccgaagc tctctaattg tctaacttcg
     2881 tcgctgtagt acat
//
LOCUS       DATMGX010000406         3145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_71197, whole genome shotgun sequence.
ACCESSION   DATMGX010000406 DATMGX010000000
VERSION     DATMGX010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71197"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..1041)
                     /gene="rplA"
                     /locus_tag="VNR65_06560"
     CDS             complement(334..1041)
                     /gene="rplA"
                     /locus_tag="VNR65_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWJ18370.1"
                     /translation="MAMDGKRLREAKKTINRVKLYPLEQAVKLVKERAKAKFDETIEI
                     AMNLGVDPRHADQMVRGVCALPNGTGRSVRVAVFARGAKADEAKAAGADVIGAEDLVQ
                     QVQGGNINFDKCIATPDMMPLVGRLGKILGPRGMMPNPKVGSVTMDVAEAVRGAKGGA
                     VEFRVEKAGILHAGIGKASFTENALVENIKAFADAVMKAKPAGAKGTFVKRVALSSTM
                     GPGVKIEPGTLSTVAHQ"
     gene            complement(1041..1469)
                     /gene="rplK"
                     /locus_tag="VNR65_06565"
     CDS             complement(1041..1469)
                     /gene="rplK"
                     /locus_tag="VNR65_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HWJ18371.1"
                     /translation="MAKKIIGYIKLQVPAGQANPSPPIGPALGQRGLNIMMFCKDFNA
                     ASKEMEPGSPVPVVITYYGDKSFSFAMKTPPASHFLKKAAGIKSGSKTPGVGSAGTVT
                     MAQVREIAEKKMKDLNATSVDQAAKIIAGSARSMGLTVKE"
     gene            complement(1595..2125)
                     /gene="nusG"
                     /locus_tag="VNR65_06570"
     CDS             complement(1595..2125)
                     /gene="nusG"
                     /locus_tag="VNR65_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HWJ18372.1"
                     /translation="MTKRWYIVHAYSNFEKKVADAIRERATAAGIDECFEEIVVPMEE
                     VVEVRRGRKVSTERKFFPGYVLVKMDMNDRAFLLIKNTPKVTGFLGADNKPQPISETE
                     AQRILNQVKEGVDRPKPSISFEIGEQVRVADGPFASFNGHVEEVDEERSRLKVAVSIF
                     GRATPVELEYGQVEKI"
     gene            complement(2162..2359)
                     /gene="secE"
                     /locus_tag="VNR65_06575"
     CDS             complement(2162..2359)
                     /gene="secE"
                     /locus_tag="VNR65_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWJ18373.1"
                     /translation="MAKVGPFEFLQQVRAETAKVVWPSRRETLVTTIMAVVMSVFASV
                     FFLLADQVFSKIIGFILTLGQ"
     gene            complement(2456..2532)
                     /locus_tag="VNR65_06580"
     tRNA            complement(2456..2532)
                     /locus_tag="VNR65_06580"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2496..2498),aa:Trp,seq:cca)
     gene            2787..2860
                     /locus_tag="VNR65_06585"
     tRNA            2787..2860
                     /locus_tag="VNR65_06585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2819..2821,aa:Gly,seq:tcc)
     gene            2946..>3145
                     /locus_tag="VNR65_06590"
     CDS             2946..>3145
                     /locus_tag="VNR65_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18374.1"
                     /translation="MPIRMAKLTRSKSGDFVSRKGIPADVRDAYSRLHRGSTKAPLRV
                     TKAGGSLTVPKVWEELFKLKGST"
BASE COUNT          648 a          976 c          813 g          708 t
ORIGIN      
        1 gtttagtggc aggccgcgtt ctcaccgcga actgccgctt cgatcacgcc gccacctcga
       61 aagaaaggcg agcgactgct caatcctgtc ccgaggcgtg atcaggcgcg atccaccaac
      121 cagccgaggc tggcgtcgag atcacctgtt atcatttccc cgtctcatgc aggcgtaaat
      181 taagccgccg ggatacccct ttggcgccag caatcttgga caggctgccg ccgtttccgg
      241 aagcagcggc caaggcgttt ccggcttggc ctatcggacg gcgaaccgcc cgaaacttgt
      301 aatgcggaac cagccagggc cggttccgcg atattactga tgagccacgg tgctaagcgt
      361 ccctggctcg atcttgacgc caggccccat cgtcgagctg agcgccacac gtttcacaaa
      421 tgttccctta gcgcctgcag gctttgcttt catcaccgca tcggcgaaag ctttaatgtt
      481 ttccacaagc gcgttctcag taaagcttgc tttgccgatt ccggcgtgca aaatccctgc
      541 tttttcgact cggaactcga cagctccgcc ttttgcgccg cgcacggcct ccgcaacatc
      601 cattgttacg ctgccaacct ttgggttggg catcatgccg cgcggaccga gaatcttacc
      661 aaggcggccc acgagaggca tcatgtcggg cgtcgcaata cacttgtcaa agttgatgtt
      721 gccgccctgc acctgctgca ccaagtcttc ggcgccgata acatcggctc ccgccgcctt
      781 cgcctcatcg gcctttgcgc cacgagcgaa aaccgccact cgcacagagc ggccggtgcc
      841 gttcggaagc gcgcaaacac cgcgcaccat ctggtcggca tgacgcggat cgacgccgag
      901 attcattgcg atctcgatcg tctcatcaaa tttggccttg gcgcgctcct tcacaagctt
      961 aaccgcctgc tccaaaggat aaagcttcac gcggttgatg gtcttcttgg cctcgcgtaa
     1021 acgttttccg tccatggcca ttactccttc accgtgaggc ccatggaacg ggcggagcca
     1081 gcaataattt tcgcagcctg atctacgctg gttgcgttca agtccttcat ctttttctcg
     1141 gcaatctcgc ggacctgagc catggtcaca gttccggctg atccgacgcc tggcgtcttt
     1201 gagccgctct tgatgcctgc cgcctttttt agaaagtggg acgccggtgg cgtcttcatc
     1261 gcgaaggaga acgatttatc cccgtagtag gtgatgacga caggtaccgg cgagccgggt
     1321 tccatctcct tcgaagccgc gttgaaatcc ttgcagaaca tcatgatgtt caagccgcgc
     1381 tgaccaagcg ccggaccgat gggcggcgag ggattggctt ggcctgcagg aacctgcagc
     1441 ttgatatagc ctattatctt ctttgccata acaacctctc aagggctcac gcccgggttt
     1501 gagggatgtg gtgcggctct tacacctgcc tggcaagcaa gcgtttgcct tccacaacga
     1561 aaagccaaag ttctgaagcc tcacgcgttt cgcgctaaat cttctcgacc tggccatact
     1621 ccagttcaac cggggtcgcc cggccgaaga tggaaaccgc aactttgagc cgcgagcgct
     1681 cctcgtccac ttcctcgaca tgcccattga aagatgcaaa cgggccgtct gcgacgcgca
     1741 cctgctcccc gatttcgaag gagatggacg gcttcgggcg atccacacct tccttcacct
     1801 gattgagaat acgctgggct tctgtctcgg aaatcggctg gggcttgtta tccgcgccaa
     1861 gaaatcccgt gaccttcggc gtattcttga tcagcaaaaa cgcccggtcg ttcatgtcca
     1921 tcttcacgag gacataaccc ggaaaaaact tccgctcggt gctgactttc ctgccccggc
     1981 gaacctccac aacctcttcc atcggcacga cgatctcctc gaagcactcg tcgatgccgg
     2041 ctgccgtcgc ccgttcacgg atagcgtccg ccaccttctt ttcgaagttc gaataggcgt
     2101 gaacgatata ccagcgcttt gtcatcactt ttgctgcttt tcgttacgat ttttttaagc
     2161 gctactggcc gagggtcaaa ataaacccga tgattttgct gaaaacctga tcggccagca
     2221 ggaagaaaac cgacgcaaag acggacatga ccacggccat tatggtcgtg actagagttt
     2281 cccgccgcga cggccacacc accttcgcag tttccgcgcg gacctgctgt aaaaattcga
     2341 aaggaccaac ctttgccatt tcttcccaac gccaatgcga agcagcttag ccttcgctat
     2401 gaaacttttt aacgtctatg cccgattgta tcgagtattc cctgccgccc ttaagtggca
     2461 ggagtggagg gactcgaacc cccaaccccc ggttttggag accggtgctc tagccgattg
     2521 agctacactc ctccatccaa ccggttacga caagcgtttt gggcacgggg gtcagccgat
     2581 ccaataccca gccggcctgc tgctgatagc tcaccagtag taggggactg attgctaaca
     2641 cgcatgcagt cgccgtgcca agcgtttttc gcgccccacc gaaacaaaat tttcatttgt
     2701 caagagccca aggaaaagct gtgcaaacct gcgttcgcgc ttttttccgt tgagcgccgc
     2761 tccaatgttg gtaatgtagg cgcgccgcgg gtgtagctca atggtagagc agcagccttc
     2821 caagctgaat acgagggttc gattcccttc acccgctcca atacaaggca cttgtccttg
     2881 tgtcgcagta atcgctgata gataggctct ttctgctcgc gctccgctac aaagggcgct
     2941 acgcaatgcc tatccgaatg gcgaaactca cccgatcaaa gagtggcgac tttgtctcgc
     3001 gcaagggtat ccctgcggac gttcgggacg cctattctcg cctccaccgt ggcagcacta
     3061 aagcgcctct cagggtgacc aaggccgggg gaagcctgac ggttccgaag gtctgggagg
     3121 aactgttcaa gcttaagggt tcgac
//
LOCUS       DATMGX010000407         2941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_371108, whole genome shotgun sequence.
ACCESSION   DATMGX010000407 DATMGX010000000
VERSION     DATMGX010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371108"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VNR65_06595"
     CDS             complement(<1..338)
                     /locus_tag="VNR65_06595"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HWJ18375.1"
                     /translation="MKALQHNMEKTMDLSLRLAEWRRLSMRGLTAVTLAIGLAMMAQP
                     AARAQGGDDAMAILKAMSDYIDAQQSIALLFDSDIEVITPELQKLQFTSSGQVLLDRP
                     NKLHVTRHGG"
     gene            complement(365..706)
                     /locus_tag="VNR65_06600"
     CDS             complement(365..706)
                     /locus_tag="VNR65_06600"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HWJ18376.1"
                     /translation="MIVFSMEIVAPDQMRTALLRTLGSILEPTRVAPGCLSARLFSDL
                     DKRKALLLVEEWDSREQFERNLDTAKVNAIVGAIELSSQAPVVRVDTVEREEGVDTLA
                     LHRTTFGGGIG"
     gene            1129..2862
                     /locus_tag="VNR65_06605"
     CDS             1129..2862
                     /locus_tag="VNR65_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWJ18377.1"
                     /translation="MIKRSIRRRIVAIAVGLIILMVATSVLSMVMVGRVGHLLDELTA
                     RYIPANTHLTRINILSLERALALRRMVIAKMQEPPDELGYKTRRQLYDAKDAEVDDEA
                     QAARKLINAIIDDTSTPSDNAALARIESRIDSLMTESRRHLKQESGELLAALEARDFA
                     AVRRSLARVDALRDELDEKIDSTRAEMVKVSYVAIATIRSEQTQAVLISVIVTILAAI
                     VGLIFANLVGGGISRPVRQLLEGTRAVEAGHLDQSIDVTTSDEIGQLAAAFNRMVIQL
                     RDNERVRETFGKYIDPRVVEGLIDRPNLTAAEGQRRVMTVLFCDMKGFTALSEGMTPQ
                     GLVKVMNRYLSIMSEPIRTNRGIIDKYIGDGIMAYWGPPFVGEADHARFACLATMEMI
                     ERIATLRREIPEMLGVRGTPMEKCDLRIGVATGEALVGSIGSDVMMSYTVMGDVVNLA
                     SRLEGANKLYGTRNLVSERTITATGGTFEVREVDRVVVAGHTHSEVVFEILGSKGELT
                     PQQVSSRDRYLEGLEAYRERRWDDALRSFNASVEAMPGDGPSMALVTRIESLKANPPS
                     EDWDGSWHIEK"
BASE COUNT          643 a          831 c          880 g          587 t
ORIGIN      
        1 tagccgccgt gccgcgtcac atggagcttg ttcggccgat ccagcaaaac ctggccggag
       61 ctggtgaatt gcagtttctg caattccggt gtgatcacct caatgtccga atcgaacaag
      121 agcgcaatcg actgctgagc gtcaatgtag tcggacattg ccttcaggat ggccatcgca
      181 tcgtcgccgc cttgggctcg tgccgcgggt tgcgccatca tcgcaagccc gatcgcgaga
      241 gtcacggccg tcagcccacg catggacagg cgccgccatt ctgcgaggcg caaagaaaga
      301 tccatcgtct tctccatgtt atgttgcaac gctttcattt ctactcccac gactgttgtt
      361 actctcatcc gattcctccg ccgaatgtgg tgcggtggag cgccaatgta tccacaccct
      421 cctcccgctc gaccgtatca acgcgaacca cgggcgcttg gctggacagt tcgatcgccc
      481 cgacaatcgc attgaccttc gcagtatcga gattgcgctc gaactgttcg cgagaatccc
      541 attcttctac gagcaaaagc gcctttcgtt tgtcgagatc cgagaaaaga cgggcggaaa
      601 gacaaccagg cgctacccgc gtcggctcaa gaattgaccc aagcgttcgc agcaatgctg
      661 tcctcatctg gtccggcgcc acgatctcca tcgagaaaac gatcaaagcc agcttctttc
      721 cgtcgccgtt ggcaggctga gcgacaaact tcgggcattg gctcgcaaga agcggaccaa
      781 gcgcaaggcc gcgtcgccgc cagcgaaaat cagcgtgaga tcaagggggt atcggatcag
      841 aacagaagct ttgacgattg ccccccgtga cagatctccg aaatgtcgga gattctacga
      901 tatgtcggag atcaatctgc cggcgcgttg agaacgtttg agtttggttt tctaccggac
      961 ggttcgggtt gaggtggatc aaaagcaagc gatggcggcc cggtaaaatt cgactaagct
     1021 ttctgcacga ccgcagacct ctccctggag gagaaagacc ttggccttgg aggaagagcc
     1081 atcttggatg ccctcacact gcaacattta aagaaattct cgggatcaat gatcaagcgg
     1141 tcgatcaggc ggcggatcgt cgccattgct gtcggattga tcatcctgat ggtggcgaca
     1201 tctgtcctgt cgatggtcat ggttggccgg gtaggccatc tgctcgatga gcttacggca
     1261 aggtacattc cagccaacac gcatttgacg cggatcaata ttctctcgct cgaacgggcg
     1321 ctcgcgcttc gccgcatggt gatcgcaaag atgcaggagc cacctgatga gttgggttac
     1381 aagacccgca ggcagctgta cgacgcgaaa gacgccgaag tcgatgacga ggcgcaggct
     1441 gcgcgcaagc tcatcaatgc gatcattgac gacaccagca cgccctcgga caatgccgcg
     1501 ctcgcacgca tcgagagccg gatcgacagc ttgatgaccg aatcccgccg tcacctgaag
     1561 caggagtcgg gggaactgct tgcggcactt gaggcacggg atttcgcagc ggtgagacgc
     1621 agccttgcgc gggtcgatgc gctccgcgac gagctcgatg aaaaaatcga tagcacacgc
     1681 gccgaaatgg tgaaggtgag ctatgttgct atcgcaacga tcaggtcgga gcagacccag
     1741 gcggtcctga tttctgtgat cgtgacgata cttgccgcga tcgtcggatt gatcttcgcc
     1801 aaccttgtcg gtggcggcat cagccgccct gtccggcaat tgctggaggg gacgcgggcg
     1861 gtcgaggcgg gccatctgga tcaatccatc gatgtaacga cgagcgacga aatcggacag
     1921 ctggccgcgg cgttcaaccg gatggtcata cagctgcgcg acaatgagcg ggtcagggaa
     1981 acattcggaa aatatatcga tccacgcgtc gtcgaagggt tgatcgaccg cccgaacctg
     2041 accgcggcgg aaggccagcg ccgggttatg acggtgctgt tctgcgacat gaaaggattc
     2101 accgccctga gcgagggcat gacgccgcaa ggtctggtca aggtcatgaa ccgctacctg
     2161 tcgatcatgt ccgaaccgat ccggacaaac cgggggatca tcgacaagta tatcggcgac
     2221 ggcatcatgg cctattgggg ccctccgttc gtcggcgagg ctgatcacgc ccggtttgca
     2281 tgtctggcca ccatggaaat gatcgaacgc atcgcgacgt tgcgtcggga aatccccgaa
     2341 atgcttggcg tgcgcggcac tcccatggaa aaatgcgact tgcggatcgg tgttgcgacg
     2401 ggagaggcgt tggtcggcag tatcggctcc gatgtcatga tgagttatac ggtcatgggt
     2461 gacgtggtga acctcgcctc tcggctggaa ggcgcaaaca agttgtatgg cacccgcaat
     2521 ctcgtctccg agagaactat caccgcgacg ggcggaacat ttgaggttcg agaggtcgat
     2581 cgggtcgtgg tagcgggtca cacccattcg gaagtcgttt ttgagatcct aggaagcaag
     2641 ggcgaactca cgccacaaca ggtgtcatca cgggacaggt atcttgaagg gcttgaggct
     2701 taccgcgaac gacgctggga cgatgcgctt cgctcgttta acgcctctgt tgaggcgatg
     2761 cccggcgacg ggccgtcgat ggcgctcgta acgcgcatcg aaagcctcaa ggcaaacccg
     2821 ccatccgagg actgggacgg gtcgtggcac atcgaaaaat aatcaccgag gcggctctct
     2881 ttagctccgc ctaacttgac gcggctcgac attgaacttc gggagcggcc tctcctggcc
     2941 t
//
LOCUS       DATMGX010000408         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_306053, whole genome shotgun sequence.
ACCESSION   DATMGX010000408 DATMGX010000000
VERSION     DATMGX010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306053"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2033)
                     /locus_tag="VNR65_06610"
     CDS             complement(<1..>2033)
                     /locus_tag="VNR65_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWJ18378.1"
                     /translation="VKVDNGTPVLLGDVARLELGPDERRGITELNGEGEVASGITLQR
                     FGLNALDVIDNVKKRIAELAASLPKGTEIIPVYDRSNLIYAAIDTLQHTLLEESAIVA
                     LVCIVFLLHIRSALVAILMLPVGILMAFASMRWLGLGANIMSLGGIAIAIGAMIDGAI
                     VMIENAHKHLERAEPGKPRTQTIIDAAKEVGPALFFSLLIITVSFMPIFTLESQEGRL
                     FGPLAFTKTFSMAAAAILSVTLVPVLMLLFVRGRIIPEQKNPINRALIWLYRPIIKGV
                     LKARVFTILLALGLLGASVWPAIKLGSEFMPSLNEGTLLFMPTTLPGISITKAAELLQ
                     TQDRIIKTFPEVASVYGKAGRAETSTDPAPTEMFETVINLLPKEEWRPGVTIESLTAE
                     MDKALQFPGVSNAWTMPIKARIDMLATGIRTPVGIKVLGKDLTELDQVAKQIETVVRN
                     VPGTTSAYAERVIGGYYLDIVPDRDALARYGLLIGNLQDTIVMALGGETVTTTVEGRE
                     RYTVNVRYPRDFRDNPEAIAREVLVPLPGGGAVPLGEVAKVQQTRGPNAIRTENGELA
                     VYIFVDMQGRDLGGYVADAQKAVADNIKFPTGAYVQWSGQFEYMQRAIAKLKIVVPLT
                     LAIIFLLLYLNFGTLTETLIVMLSLPFALVGGLWLMWWLGFNMSVAVAVGFIAL"
BASE COUNT          411 a          611 c          625 g          386 t
ORIGIN      
        1 agcgcgatga agccgacagc cacggccacc gacatgttga agccgagcca ccacatcagc
       61 cacagcccgc ccaccagcgc gaagggcagc gacagcatca cgatcagcgt ttccgtcagc
      121 gtcccgaaat tgagatagag cagcagaaag atgatcgcca gcgtcaacgg caccacgatt
      181 ttcagcttcg cgatggcccg ctgcatgtac tcgaactggc cgctccactg gacataagcc
      241 ccggtcggaa acttgatgtt atccgccacg gccttttgag catcggcgac gtagccgccg
      301 aggtcgcggc cttgcatgtc gacaaagatg tagaccgcga gctcgccgtt ctcggtgcgg
      361 atagcgttcg gccctcgcgt ttgctgcact ttggcaacct cgcccaaggg caccgcgccc
      421 ccgcccggca gaggcaccag cacctcgcgc gcaatggctt cgggattgtc gcggaaatcg
      481 cgcggatagc ggacgttgac cgtatagcgc tcgcggcctt cgacagtcgt cgtaacggtc
      541 tcaccgccaa gcgccatgac aatggtgtcc tgcagattgc cgatgagcaa gccatagcgg
      601 gcgagcgcgt cgcgatcggg cacaatgtcg agataatacc caccgatgac gcgctccgcg
      661 taggcgctgg tcgtgccggg gacattccgc accacggttt cgatctgctt cgcaacctga
      721 tccagctccg tcagatcctt gcccagcacc ttgatgccaa cgggcgtgcg aatgcccgtt
      781 gcgagcatgt ctatgcgcgc cttgatcggc atggtccagg cgttcgagac gccgggaaac
      841 tggagcgcct tgtccatttc ggcggtcagg ctttcgatgg tgactccggg ccgccattcc
      901 tcctttggca acaggttgat gacggtctcg aacatttccg ttggcgcggg atcggtcgag
      961 gtctccgcgc ggcctgcttt gccgtagacg gaggccacct cggggaaggt cttgatgatg
     1021 cggtcctgtg tttgcagaag ctccgcagcc tttgtgatcg aaatccccgg cagggttgtc
     1081 ggcataaaga gaagcgtgcc ctcattgagg cttggcatga actcggagcc gagtttgatg
     1141 gcgggccaaa cgctcgcacc caaaagtcca agcgcaagca agatcgtaaa cactctcgcc
     1201 ttcagtacgc ccttgatgat cggccggtag agccagatca gcgcccggtt gatcgggttt
     1261 ttctgttccg ggatgatgcg cccgcgcaca aacaagagca tcagaacggg cacaagcgtt
     1321 acggaaagga ttgccgcagc cgccatcgag aaggtcttcg taaaggcgag cgggccgaac
     1381 agccgtcctt cctgcgattc cagcgtgaag atcggcatga acgacacggt aatgatgaga
     1441 aggctgaaga acagcgcggg gccgacttct ttcgccgcgt cgatgatagt ttgcgtgcgt
     1501 ggcttgccgg gttcggcgcg ctccagatgc ttatgcgcgt tctcaatcat tacgattgcg
     1561 ccgtcgatca tggcgccaat ggcgatggcg atgccgccaa ggctcatgat gttggcgcca
     1621 aggccgagcc agcgcatgga ggcaaaggcc atgagtatgc caacgggcag catgaggatc
     1681 gcgacaagag cgctgcgaat gtggagaagg aagacaatgc agaccagcgc gacgatggcg
     1741 ctctcttcca acagcgtatg ctgcaaggta tcgatcgccg catagatgag gttggagcgg
     1801 tcgtagaccg gaatgatttc cgtgcccttg ggcaggctcg cagccagctc cgcgatgcgc
     1861 ttcttgacgt tgtcgatcac gtcgagggcg ttgaggccga agcgctgtaa cgtgatgccg
     1921 ctcgcgacct caccctcgcc gttgagttcg gtgatgccgc gccgttcatc gggtccaagt
     1981 tcgaggcgag caacatcccc caacaggacc ggcgtcccgt tatcgacctt aac
//
LOCUS       DATMGX010000409         2108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_332852, whole genome shotgun sequence.
ACCESSION   DATMGX010000409 DATMGX010000000
VERSION     DATMGX010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332852"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VNR65_06615"
     CDS             <1..1254
                     /locus_tag="VNR65_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18379.1"
                     /translation="PDDAPRRKPDYCLNLGITWPGLVALEIRDRVPALSFKSFGAFVA
                     GAAERASLVGDTGESDPQHWIGGFGAGRDHVLVTLHAMGPEAMTAYSERLSALFAEAD
                     AFREIWRADGMALMEVKDGESIPTFKVHFGYTDGISMTTILGGPERYPPDHQQPCEPW
                     LFVLREEAENYLVPEPRELGLNGSFAVFKMIRTDVVGFENCLQSNKDKIDPELLAAKM
                     CGRWRNGVPLALSPDSDSPANGFPAGQLNNFEYVNADGSGDPKGLRCPVGAHMRRINP
                     RGQPVTGQGQPGGTNNTHRLIRRGLPYGPVYDPNKPYDGIERGLLGYFINSSIENQYE
                     FVLGHWVNDSEFAGKVRLPPKARDPMIGTQDPAGSIFVIPQPDAAPPIKITGFSSFVT
                     TRAAAYCFLPSITAIRFMANLGPGL"
     gene            1306..>2108
                     /locus_tag="VNR65_06620"
     CDS             1306..>2108
                     /locus_tag="VNR65_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18380.1"
                     /translation="MTESDSDRNVSTEKSDIEEIAARSLLMQKNAAVAQHRSLCRGTH
                     ASGVCVRAQFEIFDVTVDRDPEMGARLAKGIFAKPGTYPSVVRFANADPNVNSDFKPD
                     VRSLSFSVHLAQDGAPVHSCPRQDFSMQNATTLPINDSPAFLATIKLLTASHPAAGLL
                     SLPFRDKLKVFRTLALAELQARQKIKPYQQLRYWSTVPFRHGPDDVVKFSAIPSRANS
                     ARPLQKDNPKGLQDELIRHINDDGPMSSFDFGIQFLDASKMTYWGKTRDA"
BASE COUNT          466 a          587 c          613 g          442 t
ORIGIN      
        1 ccagatgacg ctccgcgtcg caagccggac tactgcctga accttggcat tacctggccg
       61 gggctggttg cactggagat cagggatcgc gtgccggcgc tttcattcaa gtccttcggc
      121 gcgtttgtcg cgggcgccgc cgagcgtgcc agcctggtgg gcgataccgg cgagagcgat
      181 ccgcagcact ggatcggcgg cttcggagcc ggacgtgatc acgtcctggt tacgttgcat
      241 gccatgggtc cagaagcgat gacagcctac agcgagcgcc tctctgcgct atttgccgaa
      301 gccgatgcgt ttcgggaaat ctggcgtgcc gacggcatgg cgctaatgga agtgaaggac
      361 ggcgagtcca ttcccacgtt caaggttcac ttcggttaca cggacggcat cagcatgacc
      421 acgattctcg gtggcccaga gcgatatccg ccggatcacc agcagccatg cgaaccgtgg
      481 ctgtttgtgt tacgggaaga agccgaaaat tatctggttc cggagcctcg agaacttgga
      541 ttgaatggca gtttcgccgt gttcaagatg atccggacag atgtcgtggg attcgagaat
      601 tgcctgcaat cgaacaagga caaaattgat cctgaattac tggcagcaaa gatgtgcggg
      661 cggtggcgga acggtgtgcc gctagccttg tccccagata gtgatagtcc agcgaatggg
      721 tttccagcag ggcagctcaa caacttcgaa tacgtgaacg ccgatggctc gggcgacccg
      781 aaaggcctac gctgcccggt gggagcgcat atgcgccgca tcaacccgag aggccaaccg
      841 gtcacgggac aaggtcagcc cggtggcacc aacaatacgc accgactaat ccgccgcggc
      901 ttgccgtacg ggccggttta cgatccgaac aaaccgtatg acgggatcga gcgtgggttg
      961 ctcggctact tcatcaattc gagtatcgaa aatcagtacg agttcgtgct cggacactgg
     1021 gttaacgata gcgaatttgc cggcaaggtc aggctgccgc caaaggcgag ggatccgatg
     1081 atcggtacgc aggatcctgc gggaagcatt tttgtgattc cgcagcctga cgctgcgccg
     1141 ccgatcaaaa tcaccggctt ctcgagcttc gtcacaacgc gagctgcggc gtactgcttt
     1201 ttgccgagta tcacggcgat caggttcatg gccaatctgg gaccaggtct gtgaggtccc
     1261 ccctgcagga aagtttccat gatattattg accagatggt tcggagtgac cgagagcgac
     1321 tccgacagga acgtgtcgac tgagaaatcg gacatcgaag agatcgccgc gagatctctt
     1381 ttgatgcaga aaaatgctgc ggtcgcgcaa caccgatcac tctgccgggg aacccatgcg
     1441 agcggtgttt gtgtccgtgc ccaattcgag atatttgacg ttaccgtcga tcgcgaccct
     1501 gaaatgggag cgcgactcgc aaagggtatt tttgcgaagc ccggcacata tccttcggtc
     1561 gtgcgtttcg caaatgcgga tccgaatgta aattcggact tcaaaccgga cgtacggtcg
     1621 ctgtcgttct ctgtgcacct cgctcaagac ggagcgcccg tccatagctg tccgcggcaa
     1681 gacttttcga tgcaaaatgc gacgacatta cccatcaatg actcccccgc atttctggcg
     1741 accattaaat tgttgacggc gtcacatccg gccgccggcc tgttgtcgct gcctttcaga
     1801 gataaattga aagtgtttag gacactcgcc ttggcggaat tgcaggcacg tcagaagatc
     1861 aaaccttatc agcaactccg ctattggagc acggtgccgt tccgtcacgg acctgacgat
     1921 gtggtaaagt tctctgccat accgtctcgc gctaactccg cgcgtccact ccaaaaagac
     1981 aatccgaaag gcttgcagga tgaactgatc cgtcacatca acgatgatgg cccgatgagc
     2041 tccttcgact ttggaatcca gttcctggac gccagcaaaa tgacctattg gggcaagacg
     2101 cgtgacgc
//
LOCUS       DATMGX010000410         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_417084, whole genome shotgun sequence.
ACCESSION   DATMGX010000410 DATMGX010000000
VERSION     DATMGX010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417084"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..693)
                     /locus_tag="VNR65_06625"
     CDS             complement(271..693)
                     /locus_tag="VNR65_06625"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWJ18381.1"
                     /translation="MAIPRERFALDQLEPKARAIPYDKVCVRDLILPARIGIWSEEQG
                     ITQRVRFTVELSVAPGVHRPGDLSSVVSYDYIIDGIKDVIADGHVLLTETTAERIAEH
                     CLSHRRAVEVRVVVEKLDRIPGAALGCEIVRSRGRSER"
     gene            complement(801..1154)
                     /locus_tag="VNR65_06630"
     CDS             complement(801..1154)
                     /locus_tag="VNR65_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18382.1"
                     /translation="MNTSDENKPAASKAQPEQATERILELLDKLAAAEGLGNAASATG
                     CSEDQLRSSLRQAALIIRKKQEDPAKKRWPLLGSILDWQEMTVAEALEILADHATQQE
                     EPSPKQDDPPKKEGA"
     gene            1359..>2138
                     /locus_tag="VNR65_06635"
     CDS             1359..>2138
                     /locus_tag="VNR65_06635"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWJ18383.1"
                     /translation="MEKWFSGRGWQPHPYQKGMVHAFVNRLSTLLIAPTGGGKTLSGF
                     LPSLIDCHESRPEGIHTLYVSPLKALTNDIERNLMRPIAEMGLGVTVESRTGDTPQAK
                     RARQRRSPPNIFLTTPESLMLMLSSPDAARLFSELRAVIVDEVHSFASTKRGDFTALA
                     LSRLSALSPHHIRTGLSATVADPDALAAWLGPVGKPAHLMQSGLPTKPDIRILRAAAT
                     MPYGGFMARYAVPEIYDAIRNAGTSIVFVNTRAQAELMLQML"
BASE COUNT          393 a          682 c          604 g          459 t
ORIGIN      
        1 tcaatgcgcc gcaaacagtc tatccgaacc gcctttttga caacgatcaa tgaggttttt
       61 tactcctccc taattttttt tcgttccgcg aggcaaacgc gcaagattga agcgtccgca
      121 gcagggcggc cccgctcagg ccgtcatcgc gctcattctc cgcttctgct tctggggtga
      181 acgcgagccc atgaccggtt tcggaccctg agcagatgaa tggacttctg tgctcctggc
      241 taggcaatcg ttccgccaac atcgcggccc ttagcgctcg cttcgtcctc ttgatcgcac
      301 gatctcgcag ccaagcgccg cgccggggat ccggtcgagc ttctcgacca caacgcgcac
      361 ctcgacggcg cgccggtgcg acaggcaatg ctcggcgatc ctttccgcgg tggtctcggt
      421 caagagaaca tgcccatccg cgatgacatc cttgatgccg tcgatgatgt aatcatagga
      481 gaccacgctg gagagatcgc ccggccggtg tacgccaggc gcgacagaaa gctccactgt
      541 gaatcttacg cgctgtgtga tgccctgctc ttcgctccat atgccgatgc gggccggcaa
      601 gatgaggtcg cggacgcaga ccttgtcgta tggaatggcc ctcgcttttg gctccaattg
      661 atccagtgcg aatctctccc gcggtatcgc cattcttttg ctctcgccgt tgcaacgcgg
      721 agcacttcgc caccgcccgg atgacatcgc cattattcgg tctttgcgca acggttggat
      781 cgttggagcg gcgctccgcc tcaggcgcct tctttcttcg ggggatcgtc ctgttttggg
      841 gagggctcct cctgttgggt ggcatgatcg gcgaggatct caagcgcctc ggccaccgtc
      901 atctcctgcc aatcgaggat tgagccgagc aagggccagc gcttcttggc cgggtcttcc
      961 tgctttttgc ggatgatgag tgccgcttgc cgtaagctcg accgaagctg gtcctctgag
     1021 cacccggtgg ccgatgccgc atttcccaag ccttcagccg cggccagttt atccaggagc
     1081 tccagaattc gctcggttgc ttgttcgggc tgcgccttcg acgcagccgg tttgttttcg
     1141 tccgaagtgt tcatgaccct cacgcattac tcggtacagc cggacttgat tttatcccag
     1201 acatggaaca aaacaagaac aaagtgcaat gagattgaaa cttccgcccc acgcgaggaa
     1261 gtagccattc tggcatttca ggcagaagag cttaacattt atacaccatg ccaaagcctc
     1321 ggcccattag gtatttaccc aaaagcctgc cggactacgt ggagaagtgg ttttccggcc
     1381 gcggctggca gccgcatccc taccagaaag ggatggtgca tgctttcgtc aatcgattgt
     1441 ccacgctact catcgcaccg acgggtggcg gcaaaacgct ctccggcttt ctgccgagcc
     1501 ttatcgactg tcatgagagc cggccggagg gcatccacac gctttatgtc tcgcccttga
     1561 aggcactgac aaacgacatc gagcgcaacc tgatgcgccc catcgcggag atggggctgg
     1621 gcgtaactgt cgagagccgg acgggcgata cgccgcaggc caagcgcgcg cgccaacgcc
     1681 ggtcgccgcc caacatcttc ctcacgacgc cggaaagcct catgctcatg ctctcgtcgc
     1741 cggatgcagc gcggctcttt tcagagcttc gggccgtgat cgtggacgag gttcatagct
     1801 tcgcatcgac aaagcgtggc gactttacgg ccctggcgct gtcgcgccta tcggcactgt
     1861 cgccgcatca catccgcacc ggcctatccg ccaccgtcgc cgatcccgac gcgctggcgg
     1921 catggctcgg tccggtagga aagccagcgc acctgatgca atcgggtctg ccgacgaagc
     1981 ccgacatccg catcctgagg gccgccgcaa ccatgcccta tgggggcttc atggcgcgct
     2041 acgccgttcc ggaaatctac gatgcgatcc gcaatgcggg gacctcgatt gtctttgtca
     2101 atacgcgcgc gcaagccgag ctcatgctgc agatgctg
//
LOCUS       DATMGX010000411         3447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_173843, whole genome shotgun sequence.
ACCESSION   DATMGX010000411 DATMGX010000000
VERSION     DATMGX010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173843"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..3230
                     /gene="secA"
                     /locus_tag="VNR65_06640"
     CDS             294..3230
                     /gene="secA"
                     /locus_tag="VNR65_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWJ18384.1"
                     /translation="MINKFIAKIFGTSNEREIKRIVPLMERINVLEPETKQLSDEQLR
                     AKTEEFRQRIRERLDAVEDPDEKERELKQALDEVLPEAFAVAREASWRVLKMRHFDVQ
                     LVGGVVLHQGKIAEMKTGEGKTLVATLPVYLNALSGRGVHVVTVNDYLAKRDSEWMGK
                     LYTFLGLTVGVIVHDLDDRERREAYAADVTYGTNNEFGFDYLRDNMKFDLTDCVQRGH
                     NYSIVDEVDSILIDEARTPLIISGQSEESTDKYYRVNRIIPQLEQGEEIEKGLGEDKV
                     LTGDFVVDEKHRTITVTDVGWEKVERLLGIGNIADPENWDLKHHVDTAIKAHSLYRRD
                     VEYVVKDGEVLIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIERENQTLATVTFQNYF
                     RMYKKLAGMTGTAETEAAEFDKIYRLEVVVIPTNKVLLRVENSDVVYRTEKEKFFASS
                     DEIQKIAEKGQPILVGTTSVEKSERLSDLLKKKGIKHVVLNAKYHEKEAEIVAQAGRK
                     GAITISTNMAGRGTDILLGGNPEFISKQECIKKGLAQPLKVVAGEVTAKPDDPNLSYF
                     YYGGNEYQVPTAQWNEINARHKEVTDAEHEEVISLGGLFIFGTERHEARRIDNQLRGR
                     AGRQGDPGASRFYLSLEDDLMRIFAKEWVSNLLQRLGMEEGVPIESRLITRRIEAAQK
                     AVEGQNFEARKHLLEYDDVMNKQRQAVYGLRRQLLEGIDQKELILEDYVREILGGLLD
                     KYATKDTHAEDWDVKSLKNQLFTRFGVDIAAEGIQAEALNRQELGDAVFDKLKERYDA
                     KEKLIGPDAMRHHERIIMLSVIDQQWKDHLLSMDHLREGIGLRGYGQHDPLVEYKRES
                     FDMFEAMMERFEEETVRYLYLMQVIEAPSPAPQHAISAGDFDSNDQAVAAFSTGSRRN
                     RATTSMDDMEEAFQRRKRRELEQARMAGAGEAGTVQQVVRGEKVGRNDPCPCGSGKKY
                     KKCCGA"
BASE COUNT          811 a          941 c         1042 g          653 t
ORIGIN      
        1 gttgcgcaaa atgccgagtt tacttgtttg agtctttttt cagcagcctc tgaagccgtg
       61 ccccttcaaa gcgtgaggtc cttatgaata gtccaggtga aggcccgcac tagccgtgcg
      121 agcgaataca ccaatcctgg aaccgctcta aatgtgtcga ttccaatcct gcgaccatgg
      181 cttctgcagg gcatccaatg tgttagcctt ctacgtgggt ggagtgcgcg tgatgggcgt
      241 tttcaggccc atcgaatcaa attccaagca ggtccctgtc aaggaacaca gcattgatca
      301 acaagtttat cgccaagatt tttggcacta gcaacgaacg ggaaatcaag cggattgtgc
      361 cgctgatgga gcgcatcaat gtgctcgagc ccgagaccaa gcagctcagt gacgagcagc
      421 tccgggccaa aacagaggag ttccggcagc gcatccgcga gcgcctggat gcagtcgagg
      481 accccgacga gaaggaacgt gagctgaagc aagcgctgga cgaggttctt cccgaggcct
      541 tcgcggtggc acgcgaggcg agttggcgcg tgctgaagat gcggcacttc gatgtgcaac
      601 tggtcggcgg agttgtgctg caccagggca agatcgccga gatgaaaacc ggcgaaggta
      661 aaacgctagt tgcgaccttg ccggtttacc tcaatgcgct ttccggacgt ggagtccacg
      721 ttgtcacggt caacgactac ctggccaagc gcgactcgga gtggatgggc aagctctata
      781 ccttcctcgg cctgacggtc ggcgtcatcg tacacgatct cgacgatcgc gagcggcgcg
      841 aagcgtatgc cgcggacgtt acctacggca ccaacaacga gttcggcttc gactacttgc
      901 gcgacaacat gaagtttgat ctcaccgact gcgtgcaacg cgggcacaac tattccatcg
      961 tggacgaagt ggactcgatc ctgatcgacg aagcgcgtac cccgctcatc atcagcggtc
     1021 agagtgagga gtccaccgac aagtattacc gcgtgaaccg cattattcct caactggagc
     1081 agggtgaaga gatcgaaaag ggtctcggag aagacaaagt ccttaccggc gatttcgtgg
     1141 ttgacgaaaa gcaccgcacc atcaccgtta ccgacgtggg ctgggaaaag gtggagcgcc
     1201 ttctgggtat cggtaacatc gccgatcccg agaactggga cctgaagcac cacgtggata
     1261 ccgccattaa ggcgcactct ctctatcgcc gtgacgtcga gtacgtggtg aaagacgggg
     1321 aagtgctcat cgtggatgag tttaccggac gcctgatgcc aggccggcgc tggtccgatg
     1381 gattgcatca ggcggtggaa gccaaggaag gggtgaagat tgagcgggaa aatcagacgc
     1441 tcgccaccgt taccttccag aattacttcc gcatgtacaa gaagctggcc ggcatgaccg
     1501 gtacggcgga aacggaagcg gccgagttcg acaagatcta cagactcgaa gttgttgtca
     1561 ttcccacgaa caaggttttg ctacgcgttg agaactccga cgtcgtgtac cgcaccgaaa
     1621 aggaaaagtt cttcgcctca tccgacgaga tccagaagat cgccgaaaaa gggcagccca
     1681 ttctggtggg cacgacttcg gtggagaagt cagagcgtct gtcggacctg ctgaagaaga
     1741 agggcatcaa gcacgtggtg ctgaatgcca agtaccacga gaaggaagcc gagatcgttg
     1801 cccaggcagg ccgcaaaggt gcaattacta tttccaccaa catggccggc cgcggcaccg
     1861 acattctgct gggcggcaat cccgagttca tatccaagca ggagtgcatc aagaagggac
     1921 tggcgcagcc gctcaaggtg gtggcgggcg aagtcacggc caagcctgac gacccgaacc
     1981 tctcctactt ctactacggt ggcaacgaat accaggttcc aaccgcgcag tggaacgaga
     2041 tcaacgcccg ccataaggaa gtcaccgacg ccgagcatga ggaagttatt tcacttggcg
     2101 gcctgtttat ctttggcacc gaacgccacg aggcgcgccg catagacaac cagctccgtg
     2161 gacgcgctgg ccgccagggc gacccgggcg cgtcgcgttt ctacttgtcg ctggaagatg
     2221 acctaatgcg catcttcgcc aaggaatggg tgtcgaacct gcttcagcgc ttgggaatgg
     2281 aagaaggcgt accgatcgaa tcgaggctca tcacgcgacg catcgaggcc gcgcagaagg
     2341 cggttgaggg ccagaacttc gaagcccgta agcatctgct cgaatacgac gatgtgatga
     2401 acaagcagcg ccaggcggtt tacggattgc gccggcagct gctggaaggc atcgatcaaa
     2461 aagaactcat ccttgaggat tacgttcggg aaattcttgg tggactgctc gataaatacg
     2521 ccacgaagga cacgcatgcc gaagactggg acgtcaagag cctgaagaac cagctcttca
     2581 cccgctttgg ggtggacatc gcggccgagg gtatccaggc agaagctttg aaccgccaag
     2641 aactgggcga tgccgtcttc gacaagctga aagagcgtta cgacgccaag gagaagctga
     2701 tcgggcccga cgccatgcgg catcacgagc gcatcatcat gttgagcgtg atcgaccagc
     2761 agtggaaaga ccacttgctc agcatggacc acttgcggga aggtatcggg ctgcgcggct
     2821 acggccagca cgatccgttg gtggaataca agcgcgagtc gttcgacatg ttcgaagcca
     2881 tgatggagcg cttcgaagaa gagaccgtcc gctacttgta cctgatgcaa gtgatcgaag
     2941 ccccatctcc tgctccgcaa catgcgattt cggcgggaga ttttgacagc aacgaccagg
     3001 ccgttgcggc gttcagtacc ggctccagac gaaatcgcgc tactacgtcg atggacgaca
     3061 tggaagaagc gttccagcgc cgcaagcgcc gcgaactgga acaggcgcgc atggcgggcg
     3121 cgggcgaggc gggaaccgtg cagcaagtgg tccgcggaga gaaggtcggc cgcaacgacc
     3181 cctgcccctg tggcagcggg aagaaataca agaagtgttg cggggcgtag gagccgttgc
     3241 tcgcccttcg cccttcgctc aatacgctga gcagcggcct aattcgagcg agcagtgcca
     3301 gccttccttg tagtcccgat aaagattcct tctcttggtt tccgcttttg gtttgccata
     3361 tcggcaataa gactattatt gctttgcgtt cggggcgcga ctctgttatt gtccgcccca
     3421 aatagggagc tttgttaggg gcgcgtg
//
LOCUS       DATMGX010000412         3057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_32691, whole genome shotgun sequence.
ACCESSION   DATMGX010000412 DATMGX010000000
VERSION     DATMGX010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32691"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..597)
                     /locus_tag="VNR65_06645"
     CDS             complement(94..597)
                     /locus_tag="VNR65_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HWJ18385.1"
                     /translation="MQETALERPQTSFWKLFLVAFVVAQAIGGIGALTTDLGPWYQSL
                     NKPWWQPPDWLFGPAWTIIFALAAASAAFAWRNATTRTSRALIVVLFLTTGLFNILWS
                     ALFFQIKRPDWALIEVGALWASVLIPIFVVSRYSKKAAWLLVPYISWVTFAAILNYKI
                     VQLNPVQ"
     gene            complement(612..1805)
                     /locus_tag="VNR65_06650"
     CDS             complement(612..1805)
                     /locus_tag="VNR65_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl diphosphate reductase"
                     /protein_id="HWJ18386.1"
                     /translation="MSERDSFDVVVVGGGPSGATAADDLARLGHTVLLLDRAGRIKPC
                     GGAVPPRLIKDFAIPNEIIVARINSARMISPAGKEVDMPIDGGYVGMVDREVFDEWLR
                     NRAAESGAERRTGTFETFSRDDKGHAVVTYQTKDGEVHSVTGKVIIGADGANSAVHKQ
                     ALPDVPQVPFVFAYHEIVKSPTEGHEKYDATRCDVYYQGKLSPDFYGWIFPHGLHASI
                     GTGSMHKGFSLRNAVGELRKDSGLDDVETVRKEGAPIPLKPLKRWDNGRDVILTGDAA
                     GVVAPASGEGIYYAMHCGRIVGETVSEFLKTGDAKVLANARKRFLKAHGRVFWVLGLL
                     QHFWYRNDKRRERFVHICRDPDVQRLTWQSYMHKELVRSSPAAQARIFFKDLAHLLGL
                     VSPSR"
     gene            complement(1802..>3057)
                     /locus_tag="VNR65_06655"
     CDS             complement(1802..>3057)
                     /locus_tag="VNR65_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HWJ18387.1"
                     /translation="VELALPATLPGLLFGIHYAAEMLRPRWGYGSDKGGTRTPWIIGG
                     MLVLAISGVSAAASIALMSTNTAAGIACAILSYIGIGIGAGAAGTSLLVLIAKITSPA
                     RKPTAAALVWTMMIAGTALAASLGGRMLDPFSMERLVSVSGSISAICFTLATIAIWGI
                     ERRYLQADQASADTQPHSFFSAMREVWEEPEARRMTVFVFISMLAFSAQELLLEPFAG
                     AVFGLTPGETAKLFGSHRSGIVLGMVLGAVIGSLSRKNATAGRFWVAGGCFASAIGLF
                     ALALVGIGGAGNFLKPVVFLLGMANGTYAVAAIGTMISLASVGKGAREGTRMGLWGAA
                     QAIAFGCGGVVGTAAVDVARHFLGSPAAAYSLLFAVQGVLFFLATGLAVRLTAAAALA
                     GQEQDSELSFNASAQAVGEAGARMT"
BASE COUNT          687 a          952 c          877 g          541 t
ORIGIN      
        1 gctcgcctcg cctctcgcat aacgccgcca tatgatgacg cccgcctcaa tcaccacaaa
       61 caggattatg agatcgagaa ggatcgtcat tcgttactgc acggggttga gttgcacgat
      121 cttgtaattc aggatggcag cgaaggtcac ccaggagata tagggtacca gcagccacgc
      181 ggcttttttc gaatagcgcg acaccacaaa aattggaatt agcaccgacg cccaaagcgc
      241 tcctacctcg atgagcgccc aatccgggcg cttgatttgg aagaacaagg cgctccataa
      301 aatgttgaac aatcccgtcg tcaggaacag aacgacgatg agcgcccggc ttgttcttgt
      361 ggtggcgttt ctccaagcaa aggctgccga agcagcggca agcgcgaaaa ttatggtcca
      421 cgccggcccg aacagccagt ccggcggctg ccaccatggc ttgttgagag actggtacca
      481 cggcccgagg tcggtggtga gtgcaccgat cccgccgatc gcctgggcga ccacaaatgc
      541 gactaagaac agtttccaga aactggtttg cggtctctct aacgctgttt cctgcaaggt
      601 gttcgccgat gctagcgtga gggtgaaacc aatcccagga gatgcgcaag atccttgaag
      661 aagatcctgg cctgtgcagc agggctcgag cggacgagtt ccttgtgcat ataggactgc
      721 caagtcagac gctgcacgtc gggatcgcgg caaatatgaa cgaaacgctc gcgccgctta
      781 tcgttccgat accagaagtg ctgcagaagg cccaggaccc agaaaacccg accatgagcc
      841 ttcaggaagc gtttgcgggc gttcgccagc acctttgcgt ccccggtctt taagaactct
      901 gacactgtct cgccaacgat ccgtccgcaa tgcatggcgt aatagatgcc ctcgcccgac
      961 gcgggagcca cgacgccggc tgcatcccct gtcaagatga cgtcgcgtcc gttgtcccac
     1021 cttttcagcg gcttgagcgg gataggcgcg ccttccttcc ggaccgtttc aacatcgtcg
     1081 agccccgaat ctttacgcag ttctccgacc gcattgcgca gcgagaagcc cttatgcatg
     1141 ctgccggtgc cgatgctggc gtggagacca tgcgggaaga tccaaccgta gaagtccggc
     1201 gagagcttgc cctgatagta gacgtcgcag cgcgtggcgt cgtacttctc gtgaccttcc
     1261 gtcggggact tcacgatctc gtggtaggcg aagacgaacg ggacctgcgg cacatccggc
     1321 agcgcttgct tatgcactgc ggaattcgcg ccatccgcgc cgatgatgac cttgccggta
     1381 acgctatgga cttcgccatc cttggtttga taggtgacaa cggcgtggcc tttatcgtcg
     1441 cgggaaaacg tctcgaatgt ccctgtgcga cgttccgcgc cggactccgc tgcacggttg
     1501 cgcagccact cgtcgaaaac ctcgcgatcc accatgccga catagccacc gtcgatgggc
     1561 atgtcgactt ccttgcccgc cggcgaaatc attctcgcag agttaatgcg cgcgacgatg
     1621 atctcattcg ggatggcgaa atctttgata aggcgcggtg ggactgcacc gccgcatggc
     1681 ttgatgcggc cagccctgtc gagaaggagc acagtatgtc ccaaccgcgc caagtcgtcc
     1741 gctgctgttg cgcccgatgg accaccaccc accacgacaa cgtcaaagct gtcacgctca
     1801 ctcatgtcat tcgtgctccg gcttcgccca cggcctgggc ggatgcgtta aaggaaagtt
     1861 cggaatcctg ctcctgtccc gcgagcgcgg cagctgcggt caaccggacg gcaagacccg
     1921 tggccaaaaa gaaaaggacg ccttgaacag caaaaagaag cgaataggct gccgccggcg
     1981 agcccagaaa gtgacgcgcc acgtctacgg cagcggtgcc aaccacaccc ccgcacccaa
     2041 acgcgatcgc ttgagcagca ccccaaagcc ccatgcgcgt gccctcgcga gcgcccttcc
     2101 cgacgcttgc gagcgaaatc atggtcccga tcgctgccac cgcgtaagtc ccgtttgcca
     2161 tgccaagcag gaaaaccacg ggcttcagga aattgcccgc accgccgatg ccaaccaacg
     2221 ccagcgcaaa aaggccgatc gcggaggcaa aacagccacc cgcgacccaa aagcgtccgg
     2281 cggttgcatt ctttcggctc aagcttccaa taacggcgcc tagcaccatg ccgagcacga
     2341 taccggaacg gtgtgacccg aaaagcttcg ccgtctcgcc cggcgtgagg ccaaacacag
     2401 cgccggcaaa gggctcaaga agaagctcct gcgcactgaa ggcaagcatg gaaatgaaga
     2461 cgaacaccgt catgcgtctt gcttccggct cctcccacac ttcgcgcatt gcagagaaaa
     2521 aggagtgcgg ctgcgtatcg gcacttgcct ggtcggcttg aagatagcgg cgctcgatcc
     2581 cccaaatggc gatggttgcc aaggtgaagc aaatggcgga aatggaaccg ctgaccgaga
     2641 caagccgctc catgctgaag ggatcgagca tacgtccgcc gaggcttgcc gccagcgccg
     2701 tcccggcgat catcatcgtc cagacaagcg cggcggcggt tggcttgcgc gcagggcttg
     2761 taatctttgc gatgagcaca aggagagagg tcccggcggc gcctgctcca atgccgattc
     2821 caatgtagga aagaatagcg caagcgatcc ccgccgcagt gttcgtggac atgagcgcaa
     2881 tcgaagctgc agccgaaaca cctgatattg ccagcaccag cataccgccg atgatccagg
     2941 gggtccgcgt gcctcccttg tcagaaccgt aaccccaacg cggccgaagc atctcggcgg
     3001 cgtaatggat tccgaaaagg aggccgggaa gcgtcgcagg caaggcgagt tcgacaa
//
LOCUS       DATMGX010000413         3201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_197832, whole genome shotgun sequence.
ACCESSION   DATMGX010000413 DATMGX010000000
VERSION     DATMGX010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197832"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /gene="nifA"
                     /locus_tag="VNR65_06660"
     CDS             <1..1157
                     /gene="nifA"
                     /locus_tag="VNR65_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HWJ18388.1"
                     /translation="RLAKELSTFKTPKTSGSKLTGILGKSRAIRAVTDRIEQVAKSNA
                     PVLIRGETGTGKELFARAIHELSRRKGKPFIKVNCAALPEGVIESELFGHEKGAFTGA
                     VATRKGRFELAHGGTIFLDEIGEISPAFQAKLLRVLQEGEFERVGGQQTLKINFRLVC
                     ATNRNLEELVTEEKFRADLYYRINVVPLILPPLRDRPGDIPELAMHFLNQYAEENGHA
                     LTFGPDSIDVLQKCYFPGNIRELENCIRRTAAMTPADELHAADFACQQGHCLSSMLWK
                     GPASGEIAYTAPAQKQTRLIEAPNSAPAVPARTQHERIEPKEVVNGNGIKVVPSDENN
                     ANYSQLSKDALVKAMEKTGWVQAKAARILGLTPRQIGYALKKHGIEMKRF"
     gene            1339..1716
                     /locus_tag="VNR65_06665"
     CDS             1339..1716
                     /locus_tag="VNR65_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWJ18389.1"
                     /translation="MTIAYAICAATDIPNRRGKAFSLLRVDEDKVERPWHIFVVRWDK
                     KVFGYVNRCPHNGVNLDWETNQFLEPGSNRIVCGKHGSLFELATGKCVEGPCLGQSLE
                     PISLAVIDGDICVTGVTLAEDAA"
     gene            complement(2479..2928)
                     /locus_tag="VNR65_06670"
     CDS             complement(2479..2928)
                     /locus_tag="VNR65_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWJ18390.1"
                     /translation="MAFRVEGLYMADGRDACRIEAKLRERGLRPTRQRLDLARLLFRE
                     GDRHVTAEVLHGEALANNISVSLATVYNTLKQFTECGLLREVAIEGSKAYFDTKISDH
                     QHFYFENDGRLIDLDPGHEVMVSVPKLPEGTKIARIDVLIRLEKVKT"
     gene            complement(2939..>3201)
                     /locus_tag="VNR65_06675"
     CDS             complement(2939..>3201)
                     /locus_tag="VNR65_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWJ18391.1"
                     /translation="AGIAASSGSACASGSTEPSHVLRAMKVPYTSALGAIRFSFSHEN
                     TKDDVARVLDVLPAIVEKAREVSGFAVHGENREAGLNAAYPQ"
BASE COUNT          697 a          953 c          897 g          654 t
ORIGIN      
        1 accgccttgc caaagaactt tctacgttca agacaccgaa aacctcgggc tcgaagctga
       61 caggcatcct cggcaagagc cgggcgatcc gcgccgttac cgaccggatc gagcaggttg
      121 ccaagtcgaa tgctcctgtg ctgatccgcg gtgaaacggg caccggcaag gagcttttcg
      181 ccagggccat ccacgagctt tcgcggcgca agggtaagcc cttcatcaag gtcaattgcg
      241 cggccttgcc ggaaggcgtc atcgagtccg agctgttcgg acacgagaaa ggggccttca
      301 cgggtgccgt cgcgacgcgg aaggggcgct tcgagctggc ccatggcggc acgatctttc
      361 tcgatgagat cggagagatt tcgcccgcat ttcaagccaa gctcctgcgc gtcctgcagg
      421 aaggcgagtt cgagcgcgtc ggcggacagc agacgctgaa gatcaacttc cgcctggtct
      481 gcgcgacaaa ccggaacctt gaagagcttg ttacggaaga aaagttccgc gctgatctct
      541 attacaggat caacgttgtt ccgctcatct tgccgcctct gagagatcgc cccggcgaca
      601 ttcccgaact cgcgatgcac ttcctcaatc aatacgcgga agaaaacggc cacgccctca
      661 cctttggtcc cgactcgatc gatgtccttc agaagtgcta cttccctggg aacatccgcg
      721 agcttgagaa ctgcattcgg cgcaccgcgg ccatgacccc cgccgacgag ctccacgccg
      781 ccgatttcgc gtgccagcag ggtcactgtc tgtcgtccat gctttggaag gggcctgcga
      841 gcggcgagat cgcttacacg gctccggcgc agaagcaaac tcggctaatc gaggcgccca
      901 actcagctcc cgccgttccg gcacgaactc agcacgagcg gattgagccc aaggaagtgg
      961 tgaacggaaa cggcatcaag gttgtgccat cggatgaaaa caatgctaat tattcgcagc
     1021 ttagcaaaga cgcgctcgtc aaagccatgg aaaagacggg gtgggttcaa gcgaaggccg
     1081 cgcgcatcct cgggctcacg ccgaggcaga tcggctacgc gctgaagaag cacggcatcg
     1141 agatgaagcg cttctgagtg ggctcggctt caccggtggt ggcttgtcgc cccggaaagc
     1201 atcctggcgc ttatccggga ccgctaccca ttccaacggc gttttgcgat cccggaaacg
     1261 gctttgttcc gagataacga gcaaatgtgc agccgccgcc tctatatcag gcaacagaag
     1321 cccgactcgg agacctgaat gaccatcgcc tacgccatct gcgcagcgac agatattccc
     1381 aatcgcaggg gcaaagcctt ttctctcctt cgcgtcgatg aggacaaggt cgagaggccc
     1441 tggcatatct tcgtggtgcg ctgggacaaa aaggttttcg gctacgtcaa tcgctgtccg
     1501 cataacgggg tcaacctcga ctgggagacc aatcagtttc tggaacccgg cagcaaccgc
     1561 attgtgtgcg gcaagcatgg ctcgctgttc gagcttgcca cgggcaagtg tgtggaaggc
     1621 ccctgcctgg ggcagagcct cgagccgata agcctcgcgg tcatcgacgg cgatatctgc
     1681 gtgacaggtg tcacacttgc ggaagacgcg gcctaaagca tcgcgcactg aatcggacgc
     1741 aggtgtccga atgcttgatt gtatgcatcc cgtgtatggc ggcatctgtt ggtcaacttg
     1801 tccctgacga tttcgtccac attccttctt ctgaaagatc ctcagcgaat tcgccttgcc
     1861 ggcaggttaa cgagagctcc agcccgctcc aaaccctgat aaatatgaga taattgcaga
     1921 aaaataccga cgggacgcca tcgcccccgc ttgcgccgca agctgttccg gctgcgcacc
     1981 agcgttaatt cgcgaagccg aaacatattt aacggacttt tgctcgtccc agtaaagcag
     2041 aaaattcccg cattaaattc tatttaatgg gcactttgtt aaggataaaa agctgttcgt
     2101 tttcagtatg ttagagcgga tttctcgcgt ccacaccgca ggcgcgatgg tccatgtttt
     2161 gcgccggata taagattttt taaccaaaca tatgagcgga aataaatccg gcgcgctcct
     2221 gttaaccatg ttcgaattaa cgaccggcgc ggcgacgaac ggtttacctt cgcgatgggc
     2281 gccgtcggca atggaaagat tggcgaagca cacctgaagc atcccacgtt catttgcatc
     2341 ctgggcgtca ccgtgatcgt cgcacggacg cgcctcggcg cagtctggga tcgttgagaa
     2401 aaaattgtgt cgcaaccggc tccgatttag cgcacgccgc ggtagatcaa cccgccttta
     2461 agcgattgat ctatcaactc aggtcttcac tttctccagc cggatcaaga catcaatgcg
     2521 agcgatcttg gtcccttccg gcagctttgg caccgacacc atcacctcat gtccagggtc
     2581 aaggtcgatc aggcggccgt cattctcgaa atagaagtgc tggtgatcgg aaatcttcgt
     2641 atcgaagtag gccttcgacc cttcgatcgc gacctcgcgc aacaggccgc attcggtgaa
     2701 ttgcttcagt gtgttgtaga cggtggcaag cgagacggaa atgttgttgg caagcgcctc
     2761 gccatgcagc acctccgcgg tgacgtgccg gtcgccttcg cggaacagaa gccgcgccaa
     2821 atccagtcgc tgacgtgtgg ggcgcaagcc tctttcgcgt agctttgcct cgatgcgaca
     2881 ggcgtcccgc ccgtccgcca tgtatagacc ttccacacga aatgccattg tccctcgcct
     2941 attgaggata tgccgcgttc agtccggctt ccctgttttc gccatgaact gcgaagcccg
     3001 agacctcccg tgctttctcc acgattgcgg gcaaaacatc aagcacccgt gcgacatcgt
     3061 ccttcgtgtt ttcgtgcgaa aaagagaagc ggatggcgcc aagcgctgac gtgtagggca
     3121 ctttcatggc tcggagcaca tgcgagggct ccgtcgaccc cgacgcacag gccgacccgg
     3181 aggacgcagc gatgccagcc t
//
LOCUS       DATMGX010000414         2353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_289607, whole genome shotgun sequence.
ACCESSION   DATMGX010000414 DATMGX010000000
VERSION     DATMGX010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289607"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VNR65_06680"
     CDS             complement(<1..225)
                     /locus_tag="VNR65_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18392.1"
                     /translation="MRLQPHTSFDIDQRVRTSNSWAGGISPQHTLLILLILLSKKRAA
                     KLVDLDQGIRMANAHSLHDRDWGTNAKWIKR"
     gene            285..1958
                     /gene="ccoN"
                     /locus_tag="VNR65_06685"
     CDS             285..1958
                     /gene="ccoN"
                     /locus_tag="VNR65_06685"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HWJ18393.1"
                     /translation="MMEVASTKGTGAALFDWGTLIAALGGLILGLFIAVEAVDSVMAF
                     HAIVFSLFALAAVAFLSRKAFTSSPGVPAGYYADNVVKAATIATVFWGVVGFTMGDLI
                     AWQLAFPALNFNLPWTNFGRLRPVHTSAVIFAFGGNALLATSMYVVQRTSKARLWGRW
                     LPWFVFWGYQLFLLMAASGYVNGVTQGREYAEPEWYVDIWLTIVWVAYLLVFLGTLWR
                     RKEPHIYVANWFYLAFIVTVAMLHIVNNLAVPVSFLGTKSYSAFSGVQDAMTQWWYGH
                     NAVGFFLTAGFLGIMYYFVPKRAERPVYSYQLSIVHFWTLIFLYIWAGPHHLHYTALP
                     DWAQTLGMTFSIMLWMPSWGGMINGLMTLSGAWDKLRTDPVLRFLIVSVAFYGMSTFE
                     GPLMAVRAVNSLSHYTDWTIGHVHSGALGWVGMVSFGAIYCLVPWLWKRQGLYSQRLV
                     EWHFWTATIGILLYITAMWVSGIMQGLMWRATDANGVLQYSFVESVEAMHPYYVIRAI
                     GGVLYLTGGLIMAYNVWRTIRGDESVDLVDQPRIAAAPELRGPQAIPAE"
     gene            1968..>2353
                     /gene="ccoO"
                     /locus_tag="VNR65_06690"
     CDS             1968..>2353
                     /gene="ccoO"
                     /locus_tag="VNR65_06690"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HWJ18394.1"
                     /translation="MKLSHAPFEKYSLLLLLGILFVVSIGGLVEIAPLFYLKSTIEKV
                     DGMRPYSPLELSGRDIYIREGCYLCHSQMIRTLKDEVERYGHYSLAAESMYDHPFQWG
                     SKRTGPDLARVGGKYSDDWHVAHMTNP"
BASE COUNT          405 a          727 c          667 g          554 t
ORIGIN      
        1 cctttttatc cactttgcgt tcgtacccca atccctgtca tgcaaagaat gcgcattagc
       61 cattcggatg ccttgatcaa gatcaacgag cttcgctgcg cgttttttgc tcaggagaat
      121 caacaaaatc agaagcgtgt gctgcggcga aatgcccccc gcccaactat tcgatgtccg
      181 aaccctttga tcgatgtcaa agctcgtgtg cggttgcagg cgcatttgtc tcttccgcgg
      241 atcgcacgca aaaggctcgg ctgcgccggg attcacggga gatcatgatg gaagttgcga
      301 gcacgaaagg gaccggcgcc gctctttttg attggggtac gctgatcgcc gcccttggcg
      361 gattgattct tggattgttt atcgcggttg aggcggtcga ctcggtgatg gcgttccacg
      421 ccatcgtttt ctcgctcttc gctctcgcgg ccgtcgcttt cctctccagg aaggctttca
      481 caagcagccc gggcgttcca gcaggctact acgccgacaa cgtcgtcaaa gcggcgacga
      541 tcgccacggt cttttggggc gtcgtcggct tcacgatggg tgacctgatc gcatggcagc
      601 tcgcgttccc ggcgctgaac ttcaatcttc cctggacgaa tttcggccgg ctgcgtcccg
      661 ttcacacgtc agcggttatt ttcgcctttg gcggcaatgc tctcctggcg acgtcgatgt
      721 acgtcgttca acggacttcg aaagcgcgat tgtggggccg ctggctgcct tggttcgtct
      781 tctggggcta ccagcttttc ctccttatgg ctgcttccgg ctatgtgaac ggcgtaacac
      841 agggcaggga gtatgcggag ccggagtggt atgtcgacat ttggctgacc atcgtctggg
      901 tcgcctatct ccttgttttc ctcggcaccc tttggcgccg caaggaaccg cacatctacg
      961 tggcgaactg gttctacctc gccttcatcg tcacggttgc gatgctgcat atcgtgaaca
     1021 acctggcggt gccggtctcc ttcctcggca caaagagcta cagcgcattc tccggcgtgc
     1081 aggacgccat gacccagtgg tggtacgggc ataacgccgt gggcttcttc ctgaccgccg
     1141 gcttcctcgg catcatgtac tacttcgttc cgaaacgtgc cgagcggcct gtctattcct
     1201 accagctttc catcgtccac ttctggacgc ttatcttcct ctatatctgg gcggggccgc
     1261 atcacctgca ctacaccgcg ctgccagact gggcacaaac gctcggcatg acgttctcca
     1321 tcatgctgtg gatgccgtcc tggggcggaa tgatcaacgg cctcatgacg ctgtccgggg
     1381 cctgggataa acttcgtacc gacccggtgc tccgcttcct gatcgtctcc gtcgccttct
     1441 acggcatgtc gaccttcgaa ggtcctttga tggccgtgcg ggctgtcaac tcgctgtcgc
     1501 attacaccga ctggaccatc ggccacgttc attccggcgc gctggggtgg gttggcatgg
     1561 tatcattcgg cgcgatctat tgcctcgttc cctggctctg gaagcgtcag gggctttatt
     1621 cgcagcgcct tgtcgaatgg cacttctgga ccgccacgat cgggatcctt ctttacatca
     1681 ccgccatgtg ggtgtccggc atcatgcagg gcctgatgtg gagggcaacc gacgccaacg
     1741 gcgtgctgca atactccttc gtggagagcg tcgaggcgat gcatccctac tacgtgatcc
     1801 gcgcgatagg cggtgtactc tatctcaccg gcggcttgat catggcctac aacgtgtggc
     1861 gcacgatccg cggcgatgag tccgtggatc tcgtcgacca accacgaatc gcagcggcgc
     1921 cagagctgcg cggtccacaa gccattccgg ccgagtagga ggcgatcatg aaactcagcc
     1981 acgcaccctt cgagaagtat tctctcctcc tgcttctcgg cattctcttc gtggtctcta
     2041 ttggcggcct cgtggagatc gcgcctctct tctacctgaa gtcgacaatc gagaaggtgg
     2101 acgggatgcg cccctactcg ccgctcgagc tgtcgggccg ggacatctac atccgcgagg
     2161 gctgctatct ctgccatagc cagatgatcc gcaccctcaa ggatgaggta gaacgctacg
     2221 gccattacag cctcgccgcg gagagcatgt acgatcaccc cttccagtgg ggatcgaagc
     2281 ggacaggacc ggatctggcc cgcgtcggcg gcaaatactc cgacgactgg catgtggccc
     2341 acatgaccaa tcc
//
LOCUS       DATMGX010000415         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_162254, whole genome shotgun sequence.
ACCESSION   DATMGX010000415 DATMGX010000000
VERSION     DATMGX010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162254"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..720
                     /locus_tag="VNR65_06695"
     CDS             178..720
                     /locus_tag="VNR65_06695"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF009064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HWJ18395.1"
                     /translation="MIPKKVFLTHGVGRHREKLQSFELALRDAGIAMLNLVSVSSIVP
                     PGCKVVTKEKGLPELSPGQITFAVLARIDSCEPHRLMAASIGIALPSDKKTYGYISEH
                     HAYGETDQVAGDYAEDLAATMLATTMGLEFDPDAAWDQKRQLFTMSELIVRTTNVTQS
                     ATGDKNGLWTTVVAAAVFVP"
     gene            complement(730..2514)
                     /locus_tag="VNR65_06700"
     CDS             complement(730..2514)
                     /locus_tag="VNR65_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ18396.1"
                     /translation="MSNVSKLSTRFKRRLVTILAADVAGFCRLMGADDESTLSQLLAY
                     RQVLEDIVAAGGGRVFGVAGDSWMAEFASPVEAVRCAVECQRAIETCNGEFPEETRIR
                     YRIGIHMGDVIASSSNLFGDEVNIAARLEQFCRPGHLVVSDEVFRHVLGKVDLRFSAL
                     GVQRLKNIPTPISAFTADIIAASGPSAPQHLSTGVDVSKPVPGFQGKPALAVLPFDTL
                     GGGSDSDYLGEGFAEDLVNGLSNIRWFPVISRCSSFIFKNQAIDTASIGRALGARYLV
                     TGSVRPASKDFRLTVNLIEAANGQNLWSHKSQIGFDKFLDTQDEISARIVSLLDSEVE
                     RAEQTQLRTREVKDLDSWELIRRGIWHLYRFTKDDAPLARGFFEEALCRDPASGEARI
                     HLSWWHFWDVWTSQRDPSGLLIAKRFAREASLIDARDARAHFLMGLSQMMMGQPEEAR
                     VDYHNAIALNPSLAAAHACLGTSYIFAGQAQEGVGPLLLSLRLNPYDPFRFVFLGELA
                     VAFHMQGDWAKACEFAERALHVRPGYWYANAIRIASLARSGRIGKVLENGAPVQFSIA
                     KLNWLPFLDRKWIDYLIEGLKLAGCTLV"
BASE COUNT          610 a          772 c          752 g          490 t
ORIGIN      
        1 caaaagcagc gccgccgttt gcggagagga ccgctcagtt cacatctcag tcaacgaaga
       61 caaaccgtta cgccgttgtc ttaaactcag gcgatggtga ttgtccccgc gaaaagggac
      121 aagaatgacg atccataccg tcaccaacgt gatggcgaag ggaaacgagg tgcaagcatg
      181 atcccgaaaa aggtctttct cacgcacggg gtcggccgtc accgcgagaa gctgcaatct
      241 ttcgagcttg cgcttcggga tgccggcatc gccatgctca atctcgtgag cgtctcaagc
      301 atcgttccgc ccggctgcaa agtggtgaca aaggaaaagg gcctccccga actttcgccg
      361 gggcagatca cattcgcggt cctcgcccgg atcgacagct gcgaaccgca ccgcttgatg
      421 gccgcctcca tcggcatcgc cttgccaagc gacaagaaga cctatgggta tatttccgag
      481 catcatgcct acggggaaac cgaccaggtt gctggcgact atgcagaaga tttagcagcc
      541 accatgctgg ccacgaccat ggggctggaa ttcgatcccg atgcggcgtg ggaccagaag
      601 cggcagcttt tcacgatgag cgagcttatc gtgcggacca cgaacgtgac ccagtcggcg
      661 acaggagata agaacggctt gtggacgacg gtcgttgctg ctgctgtgtt cgttccgtag
      721 cagtctcgct caaacaagcg tgcaaccggc aagcttgagc ccctctataa gataatcaat
      781 ccatttccta tccaggaacg gcagccagtt gagcttcgcg attgaaaact gaaccggcgc
      841 cccgttttcg agcaccttcc cgatgcgacc gctccgagcc aggctcgcga tgcggatcgc
      901 attcgcgtac cagtagccgg ggcgaacgtg aagggcacgt tcggcaaatt cacaagcctt
      961 cgcccaatca ccctgcatat gaaaggcaac cgccaattct ccgaggaaga cgaagcgaaa
     1021 tgggtcatag ggattgagcc gaagcgaaag cagaagaggc cccaccccct cctgcgcctg
     1081 ccctgcgaag atgtagctgg tgccgaggca ggcgtgcgcc gccgcaaggc ttggattcag
     1141 ggcaattgca ttgtgatagt caaccctggc ctcctcaggc tggcccatca tcatctgaga
     1201 aagccccata agaaaatgag cccgcgcatc acgcgcatcg atcagcgacg cttcgcgggc
     1261 aaacctcttg gcaatgagca agcctgaggg atcgcgttgg cttgtccaca catcccagaa
     1321 atgccaccag gagaggtgta ttcgggcttc gcctgaggcg gggtcgcggc atagcgcctc
     1381 ctcgaagaac ccgcgtgcga ggggggcatc gtccttggta aacctataga ggtgccagat
     1441 accccggcga ataagctccc aggaatccag atccttgact tcacgcgtcc gcagctgcgt
     1501 ttgctccgcc cgttcgacct ccgaatcgag aaggctgacg atgcgggcgc tgatctcgtc
     1561 ctgggtgtcg aggaacttgt cgaagccgat ttgagatttg tggctccaga ggttttgccc
     1621 gttcgcggcc tcgatcaaat tgactgtaag ccggaaatct ttgctcgccg ggcgaacgct
     1681 gccggtcacg agataccggg cgcccaaggc gcggccaatc gaggctgtgt cgatagcctg
     1741 gttcttgaag ataaagctcg agcaccgtga aataaccgga aaccagcgta tattcgagag
     1801 accattgacg agatcttcgg caaacccttc ccccagatag tcgctatcgc tcccgcctcc
     1861 caaagtatcg aaaggcagca cagcgagcgc tggcttgcct tgaaagccgg gcaccggttt
     1921 cgagacgtct acgccggttg aaagatgttg cggtgcgctt ggaccggagg cggctatgat
     1981 atctgccgta aaggcggaga tgggcgtcgg aatgttcttc agccgctgca cgccgagagc
     2041 gctgaaccgg agatcgacct tgccgagaac gtgccggaac acctcatccg aaacaacgag
     2101 atggccggga cggcagaact gctccaaacg agctgcgatg tttacctcat cgccgaaaag
     2161 attgctgctg ctggcgataa catcgcccat gtgaatgccg atgcgatagc gaatacgcgt
     2221 ttcttccggg aactcgccgt tacaggtttc gatcgcgcgc tgacactcta cggcacatcg
     2281 gacagcctcg accgggctcg caaactccgc catccatgag tctcccgcga cgccgaacac
     2341 gcggccgcct cccgctgcga cgatgtcttc aaggacctgg cgataggcga gaagctgcga
     2401 tagggtcgac tcgtcatctg ctcccataag ccggcaaaaa cccgccacat cggcagcaag
     2461 aatcgtcacc agacggcgct tgaaccgagt cgagagctta ctgacgttgc tcatagcgcg
     2521 ttactaccat gcttctccgt tagaggtggg gccacgataa tcaatcgatt gcgggaaaag
     2581 cagcaaatga ccagaaagaa ataagcgcca atgtgtgtga taga
//
LOCUS       DATMGX010000416         3671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_52013, whole genome shotgun sequence.
ACCESSION   DATMGX010000416 DATMGX010000000
VERSION     DATMGX010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52013"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VNR65_06705"
     CDS             <1..237
                     /locus_tag="VNR65_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18397.1"
                     /translation="LLPAELGVHPTDEKGSHELYLMCDDLQTTIQELEGKGVKLAESV
                     KDVGWGLLTAINIPGGEVLRLYQPKHPTPLGLAP"
     gene            complement(308..694)
                     /locus_tag="VNR65_06710"
     CDS             complement(308..694)
                     /locus_tag="VNR65_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWJ18398.1"
                     /translation="MFIDYLLKTEPSAYSFADLQRDKVTIWDGVTNPVAVKHLREMKP
                     GARLIIYETGARKSAVGTASVVSVDAVDPRNPKVKIKAGRALPQPVSLAQIKANELFA
                     DSPLVRQGRLSVVPLTGAQYQALTGE"
     gene            869..2923
                     /locus_tag="VNR65_06715"
     CDS             869..2923
                     /locus_tag="VNR65_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HWJ18399.1"
                     /translation="MSTTVSTPQKKFRSYVPDSSTMPEFTLRAVLLGLVLTVILGAAN
                     AYLGLRAGMTIAATYPAAVIGMAVLRIMKGSILEENIARTVGSIGESVAAGAIFTIPA
                     FVISGAWPVFDFEHAYWKSSILMLVGGALGILFVTLLRRVMVEDPELPFPESVAASQI
                     HIAGQQGSRAAKILFGNMGFGGIVYLLGQFSLFSASRDFIVNVGTLGTSFVRMGRSAT
                     AAKLPAGAVTTMSAPAISPAYLGVGYIIGPRLASLNFAGGVIAWGLLVPLILFLGGPT
                     LVAQFAPAGTTLPDNAFWAGQAGAVWSFIVRPIAVGGMLVGACYTLFRMRKSLGSGMA
                     RAVSDLKKSAAAHAGTARTEHDLNAKFVFSGLLVVLISMVFLYKSYAASWGPGIVAAI
                     VMIIVGFFFAAVSGNLVGLIGSSNNPISGLTLAALVIAALLMVLMGVSGIHGVAAVLG
                     VAAVVCISSAVAGEMLQDLKVGHILGGTPAKMQIGDFMGVTVASFALLPVLLLLNQAN
                     INSGGIGFGDRQLPAPQAGLMAMLSRGIVGGEMAWPLIVVGILMGIALIMIEVRSPML
                     FAVGMYLPLETTFAIFLGGMIRWITDKMRDSRNFNEAQKARVENAGVLTASGFIAGEA
                     LMGIVIAVFRYKQWPLPVIFQHPNVVSGFIVLVILAVVMIRVPLANAGRPDEPAPPTA
                     IM"
     gene            3001..>3671
                     /locus_tag="VNR65_06720"
     CDS             3001..>3671
                     /locus_tag="VNR65_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWJ18400.1"
                     /translation="MTIVDDLSAVHDRIARAAQRAGRNADDIALMVVSKTQPVAKIVE
                     AYEAGQRLFGENRVPEFSDKVGALASLRDANFHMIGHLQSNKAPRAAEVFHAVDSLDS
                     AKLADRLNTAAEKLGKTLDVLIEINVGGEEAKSGLAPNSAELERIFAGAPAWTHLRVR
                     GLMTVPPFTEDPEGARPYFRQLRALRDRIAPRDLPSIKLDVLSMGMSHDFEVAIEEGS
                     TCVRIG"
BASE COUNT          662 a         1082 c         1098 g          829 t
ORIGIN      
        1 ctgctgcccg ctgaactcgg tgtccatccc accgacgaga aaggatcgca cgaactttac
       61 ctgatgtgcg acgaccttca gacgacgatc caggaacttg agggcaaagg cgtcaagctc
      121 gccgagtcgg taaaagatgt aggctggggc ttgctgacgg cgatcaacat cccaggtggg
      181 gaggtgcttc gcttatacca acccaagcac ccaacaccgt tggggctggc gccgtgaggg
      241 ccgtgctgtg gtcgccgagt caagcgccgg cacaattaag ttaaccaact caaccaactg
      301 ctctcaccta ctcgcccgtg agggcctggt actgtgctcc ggtcagtggc acgacggaaa
      361 gtcttccttg acgcaccaga ggcgaatccg cgaacagctc gttggctttg atctgcgcca
      421 acgagaccgg ctgcggaagt gctcttccgg ccttgatctt tactttcgga ttcctgggat
      481 cgacggcgtc gacagagacg accgacgcgg taccgacagc gcttttgcga gcgccagttt
      541 cgtagataat caatctcgct ccgggcttca tctcccggag gtgtttcacg gcaaccggat
      601 tcgttacccc gtcccagatc gtaaccttgt cccgctgtag atcagcgaaa gaatacgcag
      661 agggctccgt cttgagcaag taatcgatga acatgcggtc cccttctttt ttccttggtt
      721 catattttac tcatcgcgat tagccggtca ctttcgagta aagcatggca atgagcctta
      781 cctcggccca cttgcacttg tgctaagtgc cgctgacatg ctaacttgcg ccgtcgcccc
      841 tttactgcac cgcgcgaggt cttcatagat gagtaccacc gtcagcacac cgcaaaagaa
      901 gtttcgctcc tacgttcccg acagctcaac gatgccggag ttcacccttc gggccgtgct
      961 tctcggcttg gtcctgacgg tgattctggg tgcggccaat gcgtatcttg gcctgcgcgc
     1021 cggcatgacc atcgccgcca catatcccgc tgccgtcatc ggcatggcgg tgctgcgcat
     1081 catgaagggt tcaatcctgg aggagaacat tgcacgaaca gtgggctcga tcggcgagtc
     1141 ggtggcggcg ggcgcgatct tcaccatccc ggcgttcgtc atttccggcg cgtggccggt
     1201 ttttgacttc gagcacgctt actggaagtc gtccattttg atgttagtgg gcggcgcgct
     1261 cggcattctt ttcgtgactc tcctgcggcg cgtgatggta gaagatcccg agcttccctt
     1321 ccccgagtcg gtagccgcct cgcagattca catcgccgga caacagggca gccgcgccgc
     1381 caaaattctg ttcggcaaca tgggtttcgg cggcatcgtt tacttgctcg gccagttcag
     1441 cctgttcagc gcgagccggg acttcatcgt caacgtcgga acccttggca ccagctttgt
     1501 ccgcatgggt cgcagcgcta cggcggcaaa gctccccgcg ggggcggtga caacaatgtc
     1561 tgcgccggcc atcagcccgg catacctcgg cgtcggctac atcatcggtc cgcgtctggc
     1621 ttcactgaac ttcgccggcg gagtcattgc gtggggcctg ttggttccgt tgatcttgtt
     1681 cctcggcggt ccaaccctgg tcgcgcagtt cgcacctgcg ggcacgacgc ttcccgataa
     1741 cgcattctgg gcaggacagg ccggggcggt gtggagcttc atcgtgcggc ctatcgctgt
     1801 cggaggcatg ttggtgggcg catgttacac gctgttccgg atgcgtaaga gcctgggctc
     1861 gggcatggcg cgcgcggtct ctgacttgaa gaagtctgcc gcggcacacg ccggcacggc
     1921 ccgcacggaa catgacctaa atgcaaaatt tgtatttagc gggttgctgg tcgttttgat
     1981 tagcatggtc ttcttgtaca agagctacgc cgcttcgtgg gggcccggca ttgttgcggc
     2041 aattgtgatg atcatcgtcg gcttcttctt cgcggctgtc tcggggaacc tggtgggctt
     2101 aatcgggtcg tcgaacaacc ccatctccgg cctgactctg gcggccctgg tgatcgcggc
     2161 attactgatg gtgctgatgg gcgtctcggg aattcacggc gtcgccgccg tgctcggcgt
     2221 ggctgctgtc gtctgcatct cctcggcggt ggctggcgag atgcttcagg acctgaaggt
     2281 gggacacatc ctcggcggca ctcccgcaaa gatgcagatt ggcgacttca tgggcgtgac
     2341 tgtcgccagc ttcgcgctgc ttccggttct gttgctgctg aatcaggcca atattaatag
     2401 cggcggcatc ggttttggag accgtcaact ccccgctccg caagctggtc tgatggccat
     2461 gctctcgcgc ggtatcgtcg gcggcgaaat ggcatggccg ctgatcgttg tcggtatcct
     2521 gatgggaatt gccttgatca tgattgaggt cagaagtccg atgctgttcg ccgtcggcat
     2581 gtatctgccg ctggagacga cattcgccat tttcctgggc gggatgattc gctggatcac
     2641 cgacaagatg cgggacagca ggaactttaa cgaggcccag aaggcccgcg tggaaaatgc
     2701 cggagtgctg acggcttctg gatttattgc cggcgaggca ctcatgggga ttgtcatagc
     2761 cgtcttcaga tacaaacagt ggccgctgcc ggttattttc cagcacccaa atgttgtttc
     2821 tggatttatc gtgctggtga ttcttgccgt ggtcatgatt cgcgtgccgc tggccaatgc
     2881 gggtagaccg gacgaaccgg ccccgccgac agcgattatg tagagcgtgt ttatttatct
     2941 ccactgtcat cccgagcgag gacttcagtc cgagtcgagg gacctgctgt ttgtgcgcgg
     3001 atgaccatcg tcgacgatct gtccgccgtc catgaccgta tcgcgcgcgc cgctcagcgc
     3061 gcgggacgga atgctgacga cattgcgctg atggtggtct caaaaaccca gcccgtcgct
     3121 aagatcgtgg aagcttacga agccgggcag cggctgttcg gcgaaaatcg cgtgccggaa
     3181 ttctccgaca aagtgggcgc ccttgcgagc ttgcgtgacg ccaactttca catgatcggc
     3241 cacctgcaat ccaataaagc gccaagggcc gccgaagtat tccatgcggt tgattctctc
     3301 gactcggcaa agttggcaga ccggctgaat actgccgccg agaaactcgg caagactctc
     3361 gacgtgctga tcgaaatcaa cgttggcggt gaagaggcca agtccggtct cgcgcctaat
     3421 tctgctgaac tcgaacgtat tttcgctggc gcccctgcct ggacgcacct gcgcgttcgc
     3481 ggactgatga ccgtaccgcc gttcacggaa gatcctgaag gagcgcggcc ctactttcgc
     3541 caactgcggg cactgcgcga ccgtatcgct ccacgcgatc tgccgtcgat caagctcgat
     3601 gtgctctcga tgggcatgtc gcacgacttc gaagtagcca tcgaggaagg ctcgacctgt
     3661 gtgcgcatcg g
//
LOCUS       DATMGX010000417         6447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_3405,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000417 DATMGX010000000
VERSION     DATMGX010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3405"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VNR65_06725"
     CDS             <1..251
                     /locus_tag="VNR65_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ18401.1"
                     /translation="FDPERFSLEQVAARPRFSYLPFGGGRRICIGSAFAIAEATILLA
                     TIAQRYRLALVQGHRVEPQGLITLRPRYGMNMVITPRA"
     gene            724..1683
                     /locus_tag="VNR65_06730"
     CDS             724..1683
                     /locus_tag="VNR65_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HWJ18402.1"
                     /translation="MLTIHTTVAREATSSESTNPFWVDLLNPTPEETARMAAEYAIQV
                     PSRDSLQEIESSSRLRCEGQVLYVNMPLTAQDNAAGFAPLPLGFILSPHLLVTVRFSE
                     IPAFMELKKRVHQDLHMTSAAAFAALIDGMVDNSADMLEKLSSNLAAVSECVFGRNGA
                     SPAPDKRFTRSLRDSLNAVGTAGEHQSRIRESVLGLQRIIGFVSEMAAEWLPPDLKTR
                     LKTARQDLVSLVEFGEHLAGKTQFLLDAILGFISTEQNDIFKVLTIVSVVGIPPTLIA
                     SMYGMNFHNMPELGWRWGYPYALVLIVLSILLPILWFKRRGWW"
     gene            2055..3779
                     /locus_tag="VNR65_06735"
     CDS             2055..3779
                     /locus_tag="VNR65_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HWJ18403.1"
                     /translation="MSGTSQAVFMSYASQDADAAGRICEALRLAGIEVWFDRSELRGG
                     DAWDAAIRKQVKECALFVPLISAHTDARSEGYFRREWNLAVNRMLDMADDQPFLLPVV
                     IDDTSETMARVPDRFRERQWTRLPGGIPLTEFAERVTRLLAGARAAGVTTPLSGKAIV
                     SDDPGRADEGFWVAVLPFKYSGSNADLTALAEGLSEEIVTGLSRFSYLRVIARGSTLR
                     YAGQAADLRTVGKELGARYVMEGSLRQAGPRLRFAVQLVDAASGAHLWAENYERTFSP
                     ETVFELQDELVPRIVSTVADMHGVLPRSMSEAVRSRNPGELSPYEAVLRSFGYFERIT
                     GEDLAAARSGLESAVRKAPASGDAWAMLALLCVQDYAQGFNLQADALEKGLAAARRAV
                     EVAPSNHLSHYGLAQALFFLKEFQSFRNAAERAVTLNPMDGNALAFLGELLSYSGDCE
                     RGAALAARAKQLNPNHPGFYWFADFYNAYRQQDYGGALAAALNLNLPGHMGAHMVLAA
                     TYGQLGERGAAEKAVQDLLKLRPDFASIARGLLGQWWTSEYVEKLIDGWRKAGLEIAP
                     ANAAVTRP"
     gene            4042..4680
                     /locus_tag="VNR65_06740"
     CDS             4042..4680
                     /locus_tag="VNR65_06740"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HWJ18404.1"
                     /translation="MNKVASGIAHDRLKWAFFALMAGCTLLVIFVDERFIVDFQDPNW
                     QHIARYKWWLLVHGVPAATALVVGPFQFSDTLRRTRLNLHRWTGRIYIGAVAIAAPVG
                     GYIGTNFMRPILAAEQPAQSGLWFATTVMALICVLNRNIGAHRVWMMKSYCFCLLFVL
                     SRVPDAIPVKWSDAGIAAFLWYLIVAALVGPDVVVTVRELWRRRAPAVSLTR"
     gene            5041..5805
                     /locus_tag="VNR65_06745"
     CDS             5041..5805
                     /locus_tag="VNR65_06745"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HWJ18405.1"
                     /translation="MSGDAIVMLGEVHDNAEQHHLRLERLQKAFAEGWRPVIVMEQFD
                     RQHQEDIDRARRERPKDVQYLLDLGAPRDTEGGNWDWKFYRPYVALALEYDVPLVAGN
                     LSRADARKVVKDGYSAVFDPDTLVSLGLNEPIALDWESAQEREIEVGHCDQLPPSILP
                     AMARAQFVRDAVMASAVKANAGHGLVLLAGNGHVRRDIGVPRWLSPPLRSRVFSVGYL
                     EKGNTSIARAFDAVVITAAASRSDPCLKFGKPVTGP"
     gene            6003..>6447
                     /locus_tag="VNR65_06750"
     CDS             6003..>6447
                     /locus_tag="VNR65_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWJ18406.1"
                     /translation="MDRIALELNIEVIDLAIQRMSSYDYEHLNRNDDFEPLMKHVLEH
                     DQIIFATPIYWYAVSPAMKVFLDRISDLLELPDLLSEGRRLRGKNAYVVCTSISDEPS
                     AAFIEAFRETFDYLGMRFGGVAHVNCQDGYLPAAHDSEAMKFAALV"
BASE COUNT         1254 a         1821 c         1997 g         1375 t
ORIGIN      
        1 ggttcgaccc tgagcgcttc tcgctggagc aagtcgccgc gcgtccacgc ttctcgtacc
       61 ttccattcgg cggcggacgt cgtatctgca taggatcggc tttcgccatc gcggaagcga
      121 cgattctgct cgccacgatc gcacagcgct accgactggc gctcgtgcag gggcatcgcg
      181 tcgagccgca gggcctgatt acgcttcgcc cccgctatgg gatgaacatg gtgatcaccc
      241 cgcgggctta acgaaccagc aggcgcgccg acgatttcgc tcaggccgtc cgcttgagag
      301 gagggccgcg ataccatcgc attgaattaa gcttccggta agccgaccgt cgccgcagac
      361 agcagcgggt cagagttgcg cgttgccggc cgctgcttat ttgaacagaa tgaactcgcg
      421 ttcggctgac gaccgcaacc aatattagca attctgttca atagaatcgc acatgttgca
      481 tctgcgtacg ctatcaacga aattacacgg tgatatcgaa ctacatccgg gaaacgtgat
      541 ccacacatgc gttgtttgag cattttcatt ctgtcccgtg gcgcatgaac tgagcacgat
      601 agtcacgagg aggcacgatt aacctggctg gcgcgtccga tcgcatccgc ggtagttggg
      661 aatgagccgt tttcgtgtca gtatcgatgc cgatccccct ttttggttat ttgggctggc
      721 cttatgctga ctatccacac cactgttgcc agggaagcaa ccagttccga gagcacgaac
      781 cctttttggg tcgatttgct caatccgacg ccggaagaga cagcgcgaat ggcggcagag
      841 tatgccattc aggttccgtc ccgagattcg ttgcaggaga tagagtcctc cagccggctc
      901 aggtgcgagg ggcaggtact gtacgtcaat atgcccctca cggcgcaaga taatgcggcc
      961 ggcttcgcgc ccctgccgct cggatttatt ctgtcgccgc accttctggt caccgtgcgc
     1021 ttttcagaaa tccccgcctt tatggagctt aagaagcgag ttcaccagga tttgcatatg
     1081 accagcgccg ccgcgttcgc tgcgctgatc gacggcatgg tggataacag cgcagatatg
     1141 ctcgaaaagc tgagcagcaa tctagccgct gtatcggaat gcgtcttcgg aagaaacggc
     1201 gcttctccgg cacctgataa gcggtttacc cgctctctcc gggacagcct caatgcggtc
     1261 ggcacggcgg gggagcatca gtcacgcatt cgcgaaagtg tccttgggtt gcaacgaatc
     1321 attgggttcg tctccgaaat ggcggcggag tggttgccac cggatctgaa aactcgcttg
     1381 aagactgcgc ggcaagatct tgtctcgctg gtcgaattcg gagaacacct ggcgggcaag
     1441 acacaatttc ttttggatgc cattctcggt tttatcagta ctgaacagaa cgacattttc
     1501 aaggtgttaa cgatcgtatc cgtggtggga attccgccca cgctgatcgc cagtatgtac
     1561 ggtatgaatt ttcacaatat gccggaactc ggatggcgtt ggggctatcc ctacgcgctg
     1621 gtgctcatcg tgctcagcat cttgctgccg atactgtggt ttaagcggcg gggttggtgg
     1681 taagcgggaa gtcgcgatag cgtgcgtccg agcctatgtt ggcgcatggt tccgaggccg
     1741 cgaaaacaat cggtgggtac ggaaaccgta acgcgacgcc gtgttaagtg tcgagatgtc
     1801 cgcatcgttg ggatctgcat ggcgggcgct cgtgactagt tcgttagggt cgacacgagg
     1861 cgatcagccc tcacttggag ttttgcgagc ggcgaccgac tccgttcgcg caaagcccgt
     1921 cggttgcgat gctgaaatga ttgcccgatt acggacgttc cgagtccgaa atccgtcgcg
     1981 gttgccaagc cgcggagcat ccgccacgat tcgggccggg taagggagaa actcggcatg
     2041 gtcggaagcc tataatgagc ggcacttcac aggccgtctt catgagctat gcctcgcagg
     2101 atgcggacgc ggccggccgt atctgcgaag cactgcggct agccggcatc gaagtctggt
     2161 tcgaccggag cgagttacgc ggcggcgatg cgtgggacgc cgcgatccgc aagcaggtca
     2221 aggaatgcgc tctcttcgtg ccgctcatct ccgcccatac cgatgcgcgc agcgaaggct
     2281 acttccgacg cgaatggaat ctcgcggtga atcgaatgtt ggacatggcg gacgaccagc
     2341 cgttcctgct gccggtggtg atcgacgaca cttcagaaac gatggcccgt gtacctgacc
     2401 ggtttcgcga gcggcaatgg acgcggctgc cgggcggcat accgctcacg gaattcgccg
     2461 aacgtgtgac gcgcctgctc gcgggcgctc gagcggctgg ggttactaca ccgctgtctg
     2521 ggaaggcgat tgtttcggat gatccggggc gcgcggacga gggcttttgg gttgcggtgc
     2581 tgcccttcaa atacagcggc agcaacgccg acctcacggc cttggccgaa ggactgtcgg
     2641 aagagatcgt caccggtctg tcccggtttt cttatctgcg ggtgatcgcc cgcggctcga
     2701 cattgcggta cgcaggccaa gcagcggatc tgcggactgt gggcaaggaa ctgggcgcgc
     2761 gctacgtgat ggaggggagc ctgcgccagg cgggaccgag gctgcgcttc gcggtccagc
     2821 tcgttgacgc ggcctccggc gcccatcttt gggccgagaa ctatgaacgc accttcagcc
     2881 cggaaaccgt gttcgaactg caggacgaac tggttccgcg aattgtttcg acggttgcgg
     2941 atatgcacgg ggttttgccc cggagcatga gtgaggctgt gcgcagccga aatcccgggg
     3001 aactcagccc ctacgaagcg gtgctgcgca gtttcggcta cttcgagcgg atcaccggcg
     3061 aagatcttgc cgctgcgcgc tccggactgg aatcggcggt acgcaaggcg ccggcttccg
     3121 gcgatgcctg ggccatgctg gcgctgcttt gcgtccagga ttacgcgcaa ggattcaacc
     3181 ttcaggccga cgccttggag aagggattgg cggccgcgcg gcgtgccgtt gaggtggcgc
     3241 catccaatca tctgtcccat tacggtctgg cccaggcgct attctttctg aaggaattcc
     3301 agagcttccg caatgcggcg gaacgggccg tcacgctcaa tccgatggac ggcaacgccc
     3361 tcgcgtttct tggcgaactg ctgtcgtatt cgggcgactg cgagcgaggc gcggcacttg
     3421 cggcacgcgc aaagcagctg aatccgaatc accccggctt ctactggttt gcagacttct
     3481 acaacgcgta ccgccagcag gactacggcg gcgcgcttgc tgcggcgctc aatctcaatc
     3541 tgcctggcca catgggcgcg catatggtgc tggctgccac ctacggccag ctcggggaac
     3601 ggggggccgc ggagaaggcc gtgcaggacc tgctcaagct gcggccggac tttgcctcca
     3661 tcgcacgcgg tctcctgggg cagtggtgga cctccgagta cgtcgagaag ctgatcgacg
     3721 gttggcgcaa ggcggggcta gagattgcac cggcaaatgc cgcggtgaca cggccgtaac
     3781 cgtatacgat acgtgcagcg accgctccgc tgcgatgcgc gacagtccgc tcatggacaa
     3841 cgcgaccacc cggcttgggt ctcgggagct gtcgtttgcc gataacccta ccttctgcgc
     3901 gctcaatgtt ggctctcggg acaagtatca ggcgcttccc ggccaagagc gggcgttgcc
     3961 ggcacacaat tggaaggtcc caagcagaca gtggtcgcct ccagtaagct atctttcttg
     4021 atggctggga ggtggagctg tatgaacaaa gtcgcgtctg ggatcgcgca tgaccgcttg
     4081 aaatgggcat tcttcgcact gatggcaggc tgcactctgc tggtcatttt tgtagatgaa
     4141 cgatttatcg tcgacttcca agatcccaat tggcaacaca tagcgcgata caaatggtgg
     4201 ttattagttc acggcgtgcc ggccgcgact gcccttgttg ttggcccttt ccaattctcg
     4261 gatacgctgc gtcgcacccg gctaaatctt caccgctgga ccggccgtat ttatatcggc
     4321 gcagtcgcta tcgccgcacc ggtcggcggt tatattggta cgaactttat gagaccaata
     4381 ctggcggccg agcagcccgc gcagtccgga ctatggttcg cgacaacggt gatggcgttg
     4441 atctgcgtcc taaaccgaaa tatcggggcg caccgagttt ggatgatgaa gagctattgc
     4501 ttctgcctgt tgtttgttct ctcgcgcgtg ccggatgcga tcccggtcaa atggagcgat
     4561 gctggaatcg cggcctttct gtggtatttg attgtcgccg cgctcgtggg gcctgacgta
     4621 gtcgtgacgg tgcgcgaatt gtggcggcga agggcgccgg cggtttcgct tacgcgatag
     4681 cggacgttcg acaccgtcag cttccggtcg acatgcgacc catgccggtg acccccgttt
     4741 ttccaggccg agtgaccggt atctggatgg acgtccggca aaaatcggcc atgacccgtc
     4801 gtagactgaa ttggaattac agcccgaaag cggtcgcccg cggcttggtg tagtctctaa
     4861 cgaatgtcgt actttcattc acttggagga ttggcgatct tcctcgccct cggtgcgaca
     4921 acaatgcggt cgctacgacc agccaggaat ttacgtagcc tacggtacat cgcagcggcc
     4981 ctgctcgtca gcctgtgcgc gtgcgccagc accgcccccc atcgtgattg gcaatcccga
     5041 ttgtcgggtg acgctatcgt gatgctcggc gaggtgcacg ataacgccga gcaacatcat
     5101 ttgagactgg agcgattgca gaaggccttt gcggaaggat ggcggccggt gatcgtcatg
     5161 gaacaattcg atcgacaaca tcaggaggac attgatcgtg cccgccgaga aaggcccaag
     5221 gatgtgcaat acctgctcga tcttggggcg ccacgcgata ccgaaggagg gaattgggat
     5281 tggaagtttt ataggccgta tgtggcgctc gcgcttgagt acgacgtgcc gttagtcgcg
     5341 gggaatctct cgagggcgga cgcccgcaag gttgtcaaag acggatattc agcagttttc
     5401 gacccggaca cgctggtgag tcttggtttg aacgagccga ttgcactgga ttgggagagc
     5461 gcccaggagc gcgagatcga ggtcggccac tgcgaccagc taccgccatc cattctacca
     5521 gccatggcca gagcgcaatt cgtacgggat gccgtgatgg cgtcggcggt gaaggcgaat
     5581 gcaggacatg gcctggtatt gctggcaggc aacggtcatg tcagacgcga cattggcgta
     5641 ccgcgctggc taagtccgcc gctgcgatcg cgcgtattct cggtgggcta tctcgagaag
     5701 ggaaatactt ccatcgcaag ggcattcgac gctgtggtga tcaccgccgc cgcatcacgg
     5761 tctgatccct gtctcaaatt cggcaagccg gttaccggtc cataggcagc aggctctgcg
     5821 tagggtggca cttccggcga tagaactgac gtcgacgggc cttgatccag gcgaatagtt
     5881 gcgctcagaa tgacgggcag ccctaagcga gcatcgacag cggcaaaccc aaggtccgaa
     5941 ctatgctgag cacgatagct ctgttttcca gttcgcgccg ccacggaaat acggctcgac
     6001 tgatggaccg cattgcgttg gagctgaata tcgaggttat cgatctcgcc atccagcgca
     6061 tgtcatcgta tgactacgag cacctcaata ggaatgatga cttcgagcct ctcatgaagc
     6121 atgtgttaga gcatgatcag atcatctttg cgacgccgat ctattggtat gcagtaagtc
     6181 cggcaatgaa ggtatttctc gatcggattt ccgatctcct tgaattaccg gacctgctgt
     6241 ctgagggtcg ccggttgcgg ggaaagaatg catacgtcgt gtgcacatcg atcagcgacg
     6301 aaccgtccgc ggcattcatc gaagcgttcc gcgagacctt cgattacctc ggcatgcgct
     6361 ttggtggcgt tgcgcacgtg aactgtcagg acggatatct acccgctgcg cacgattcgg
     6421 aggcaatgaa attcgcagcc cttgtga
//
LOCUS       DATMGX010000418         3547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_181544, whole genome shotgun sequence.
ACCESSION   DATMGX010000418 DATMGX010000000
VERSION     DATMGX010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181544"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VNR65_06755"
     CDS             complement(<1..240)
                     /locus_tag="VNR65_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HWJ18407.1"
                     /translation="MKSIEQRIAEELGVRPQQVQAAVELLDGGSTVPFIARYRKEVTG
                     MLDDAQLRTLEERLGYLRELEERRTAILDSIREQGK"
     gene            948..1361
                     /locus_tag="VNR65_06760"
     CDS             948..1361
                     /locus_tag="VNR65_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HWJ18408.1"
                     /translation="MELRRLNGQLAVAGQINPGDVPELAAEGIRAIICNRPDGEAPGQ
                     PKYAEIEKAAAANGIKIAYQPVVTTSISDEDAVTFGKLIEELPKPLLVYCRSGLRSTA
                     LWALSQSGKLPVEDIVETAAKAGYDIRPFLLHLVR"
     gene            1369..2028
                     /gene="bluB"
                     /locus_tag="VNR65_06765"
     CDS             1369..2028
                     /gene="bluB"
                     /locus_tag="VNR65_06765"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: protein motif:HMM:TIGR02476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HWJ18409.1"
                     /translation="MSGDTAANAIPEFDAGFRAKLADLVLWRRDVRKFRPDPLPAGSM
                     DRLLRLACLAPSVGLSEPWRFVLVDDPAKRELMRKNFKAANAEALASYSGDRATLYAA
                     LKLEGLDDAPVQLAAFSDPDPEQGAKVGRRTMPETVAYSVVTAIHTLWLAARAAGIGV
                     GWVSILDPAPVARDLEVPLHWQFIGYFCVGYPMEVADTPELERANWQKRADPSSFIFR
                     R"
     gene            complement(2444..>3547)
                     /locus_tag="VNR65_06770"
     CDS             complement(2444..>3547)
                     /locus_tag="VNR65_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HWJ18410.1"
                     /translation="QALKAYQAPPQNIVYADREGHIGFINPGLLPIRKAGDGRMPADG
                     ASGAFDWTSMASFDQMPQVRDPEAGFILNANSAVVPRNFSPTLGSDWGEPFRARRIQQ
                     FMDEDGAHTLDTSARMQADHLSLAAKDLLPALLAVTPATPQAKEALALLQNWDGVMDK
                     DRPEPLIFEAWLHEMHRLLLTAKTGDPLGEKGPFAAASIEFILANRAAEWCGKDDADC
                     AKLKTEALNQALAMATKRQGPDTKTWRWGRENVALLRHKFYSRIPLLDRLSDLSVESS
                     GDFYTLDRGGGTEHDADLPFARSHGTGYRGIYDLANPDASRFIIATGESGHIFSRHYG
                     DLVPLWNEGRTITLAGSEEELKAQGAEGLIFKP"
BASE COUNT          753 a         1004 c         1030 g          760 t
ORIGIN      
        1 cttgccctgc tcgcggatcg aatcgaggat cgcggtgcgg cgctcctcca actcgcgcag
       61 atagccgagc cgctcctcca gcgtgcgaag ctgcgcgtcg tcgagcatgc cggtgacttc
      121 tttgcggtag cgcgcgatga agggcaccgt cgagccgccg tccagaagct cgaccgccgc
      181 ctgcacttgc tgcggcctaa cgccaagctc ttccgcaatc cgctgttcga tggacttcat
      241 cgtttaacgc tccgctgtgg tgttgcagcc cgcgagggcg gctcgaatcg agcggcgatc
      301 atacgcaggc ggcggcgcga ttcaaaacac ggctttgtaa tattcacggc gaagttgcgc
      361 tccggagggg ccgaatggat ggagtggcgc ggccacttcg tcgctttcgg cggaaagcca
      421 atatgagccg acgcgccaat tctttgccgc aagaatggtg gcttaagatg ctgagatgca
      481 atgaaagcat tccacgttcc gatctgtaaa atgtggctag cagcttgctc tgtctagtca
      541 cagccgcggt aacattgcca tgcgaagccc aaatgagctg tcagaaacgg aacaacgagg
      601 gccagcctcg cattcttttg catcgcgggc agagataacc aagagccaca acagcggcgt
      661 ctgacaatgg tagcgcctcg ctccaattgc ttcgcttacg aacaagaggc ttgccaacag
      721 atctaatttt taatttcttg acatgtacta tattttatct tttcaaaact tcacttttga
      781 cactaataat ttttacaagt agtactgcta cacacacaaa aaagagccat aaatgctgat
      841 gctaaagaaa tttatcccaa agatggtgtg aaaagtgcta ttgatctagc aaatttctct
      901 cattttgctt agggtggaat gaaaccagat cgaaaggggt cacgataatg gaactgcgca
      961 gactcaacgg ccaactcgct gtagccggcc aaatcaaccc gggcgatgtc ccggagctgg
     1021 ctgccgaggg catccgcgca atcatctgca atcggcccga tggggaagcg ccgggtcaac
     1081 ccaaatatgc ggagatcgag aaagctgcgg cggcaaatgg gatcaagatc gcctatcagc
     1141 ctgtcgttac cacttcgatc agtgatgagg atgcggtaac ttttggcaag ctgatcgaag
     1201 agcttccgaa gccgctgctc gtttactgcc gttcgggcct gcgctctacc gcgctttggg
     1261 cgctgagcca gtccgggaag cttcccgttg aagatatcgt cgagacagcg gccaaggccg
     1321 gctacgacat caggccattt ttgctgcatc tggtgcggtg acccgatcgt gagcggcgat
     1381 acggcggcga acgccattcc agaattcgat gcgggctttc gcgcaaagct ggcggacctt
     1441 gtgctttggc ggcgagatgt ccggaagttt cgtcccgatc cacttccggc cggaagcatg
     1501 gatcgccttc ttcggcttgc gtgtcttgct ccttcggtcg ggctgagcga accctggcgc
     1561 ttcgtgctcg tagatgaccc cgcaaagcgc gagcttatgc gcaagaattt caaagcagca
     1621 aacgcagagg ctctcgcgtc ttacagcgga gatcgggcga cactctacgc ggccctgaaa
     1681 cttgaaggac tggatgatgc gcccgtgcaa ctggccgcgt tttcggaccc cgatcctgag
     1741 cagggcgcga aggttgggcg gcgcaccatg ccggagaccg tcgcttattc cgttgtgacg
     1801 gccattcaca cgctttggtt ggctgcgcgg gctgcaggga tcggcgtggg ctgggtttcc
     1861 attctcgatc cggcccccgt cgcgcgcgat ctcgaagtac cgctgcattg gcagttcatt
     1921 ggttattttt gcgtgggcta tccgatggag gtcgcggaca cgccggagct ggaacgggcg
     1981 aattggcaga agcgcgccga cccgtcttca ttcatcttcc gccgctgaag aggatgcttg
     2041 atacggcagc tcatccttca gttgccgaag gtctggcaaa cagctggaat ggccgctgca
     2101 ggcctgctct tttagacgcg ccgtggctat ccggcgcgtc tgccagtccg gggtgaagca
     2161 tcaaagtgtt ggggaagcca gaaatttgct taggcggcag caaggcgcgc cgcaaaaatc
     2221 gagttaaccc cacgctaacg cattcaccct aaactaacca tcaaaatctt gaaatgttac
     2281 ttcaatttcg agcaaaaaga agcaattttt gcaaaaccaa cgaatcgcga cgccgtagat
     2341 aaaggcgttt gagagaggta tctatatatc tatttatagc ccgttttacg aaacgaaacc
     2401 aattttcacc cgattttaag caaggccgct atccacacgc tcgtcagggc ttgaagatca
     2461 acccttcggc cccttgtgct ttcagttctt cctctgaacc cgccagcgtg atggtgcgcc
     2521 cctcattcca caacggcacc aagtccccgt agtggcgcga gaagatatgg ccggattcgc
     2581 ccgttgcgat gatgaagcgc gaggcatccg gattggcaag gtcatagatg ccgcgatagc
     2641 cggttccgtg ggagcgggca aatggaagat cggcatcgtg ttcggtgccg cccccacgat
     2701 cgagcgtgta gaagtcgccg ctgctctcga cgctgagatc ggacaggcgg tcgagaagcg
     2761 gaattcggct gtagaatttg tggcggagca gcgcgacgtt ttcacgaccc cagcgccagg
     2821 tcttcgtatc cggcccctgc cgcttggtcg ccatcgccaa agcctgattc agggcttccg
     2881 ttttcagctt tgcgcagtcg gcatcgtctt tcccgcacca ttctgcggca cgattggcca
     2941 agatgaattc gatagaagca gcagcgaaag gacccttctc gcctaacggg tcgcctgtct
     3001 tcgcggtcag caagaggcga tgcatctcat ggagccacgc ctcgaaaatc agcggctctg
     3061 gacggtcctt atccatgacc ccgtcccagt tctggagcag ggccagcgcc tccttcgcct
     3121 gtggggtcgc gggcgttacg gccagcaacg cgggcagcaa atccttcgcc gccagcgaaa
     3181 ggtgatcggc ctgcatccgc gccgatgtgt cgagcgtatg cgcgccatct tcatccatga
     3241 attgctgaat tcggcgggcg cggaacggct ctccccaatc cgatccaagg gtgggcgaga
     3301 agtttcgcgg aacgacggcg ctattcgcgt tgaggatgaa tccggcttcg ggatcgcgga
     3361 cctgcggcat ctgatcgaaa gaggccatgc tggtccaatc aaacgcaccg gacgcgccat
     3421 cggcgggcat gcgtccatcg ccggccttac ggataggtag aaggccggga ttgatgaacc
     3481 cgatgtgacc ttcgcgatcg gcgtagacga tgttttgcgg cggggcctga tacgctttca
     3541 aagcttg
//
LOCUS       DATMGX010000419         3463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_128636, whole genome shotgun sequence.
ACCESSION   DATMGX010000419 DATMGX010000000
VERSION     DATMGX010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128636"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1956
                     /locus_tag="VNR65_06775"
     CDS             <1..1956
                     /locus_tag="VNR65_06775"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18411.1"
                     /translation="IVRDTVVAQATPPKFLMLGDSSSLHLSIENVEAPAGTYQLTAKG
                     DGGVEVVGPAERTLTLDLEKRVSQTIPLKGASVGDGHVSFSLTGPGNVAIERTYAIAV
                     EPPAPNVRRRTTQMLVATTGSLRVGSELIRDLVPATAKISLTASRTASFDVPGLLIGL
                     DRYPFGCAEQTTSRALPLLYYDEVASRARISKEPKAKAAIEKAIARLYEMQSSSGAFG
                     LWGPSGEDMWLTAYVTDFLTRAREKGYAVRDTNYEISLDRLKNTVNNARDFKKGGEDL
                     AYALYVLARSGRGVLGDLRYYADTKIDAFSTPIARAQLAAGLAMLGDKERATLTFASA
                     LSALVTPDPQVPLAYRADYGTQLRDAAAVLALMGESGATTPQLQKAFNIVVKLRAQQK
                     ETTTQESLWLLLAARTLDAQNKDLALEVNGAPVKGSFQTVLSGSDFGGPIQITNVSAI
                     LPNAASETFQTMLTPAQFTAQGLVIANRGPEAVPASVLVTGEGLEPEPSAESGFKIER
                     KTYAPDGREIPFDKLKQNDRVVVVLKVTEVDPKLAQIVVEDRLPAGFDIENPALLKGS
                     DLKAFSWLPSTYTPVFSAFRDDRFVAAFSLTDTGRKVPAQMTMAYVMRAVSPGVYTHA
                     GARVEDMYRPDRFARTSGAKVEITAAQ"
     gene            2091..>3463
                     /gene="pbpC"
                     /locus_tag="VNR65_06780"
     CDS             2091..>3463
                     /gene="pbpC"
                     /locus_tag="VNR65_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HWJ18412.1"
                     /translation="MSERKTHKWFIRGALVFALVVLGASSLKLFDPLPLEVAASSSRL
                     VLDRDGNLLRAFTTPDGRWRLDARPEDVSPTYLALLFAFEDRRFWRHPGIDPLAFGRA
                     ILQAITHGKPISGGSTLTMQVARLLRGSPTHSVSAKFQQVADAARLEWTLSKRQILSL
                     YLKLAPYGGNLEGVRAASLAYFGKEPQRLSIAEAALLVTIPQSPEVRRPDRGYESLVD
                     GRNRVIRRAAAAGAITQADAKAALAQAVPRGRRDFPALAPHLSERLTVTLPNLSAIAT
                     TIDRNLQEAAETITRRNAQSFGEKISIAALMVSNRTGEVLAHVGSAGYFDGSRLGAID
                     MTTAIRSPGSALKPFIYGLAFEDGLAHPETLIEDRPVRFDGYAPVNFDNVFHGTVTVR
                     TALQLSLNVPAVKVLNEVSPVKLAARFREAGMPFAVPRNLTVALGGAGLTLENLTTLY
                     VGLARGG"
BASE COUNT          636 a         1100 c         1073 g          654 t
ORIGIN      
        1 attgtccgcg acacggttgt ggcgcaggcc acgccgccca agttcctgat gctcggcgac
       61 agttccagct tgcatctcag catcgagaac gtggaagccc ccgccggcac gtaccagctg
      121 acggcaaaag gcgatggcgg agtcgaggtt gtcggtcctg ccgagcgcac gctgacactc
      181 gatctcgaaa agcgcgtgag ccagaccatc ccgctcaagg gcgcgtcggt tggcgatggg
      241 catgtctcct tctcgcttac gggaccgggg aatgtcgcca tcgagcgcac ctatgccatc
      301 gcggtcgaac cgcccgctcc gaatgtacgg cgccgcacga cgcagatgct cgtcgccaca
      361 acgggctcgc tgcgtgtcgg cagcgaactg atccgcgatc tcgttccggc gaccgcgaag
      421 atctcgctga ccgccagccg cacggcatcg tttgatgtgc ccggacttct gatcggcctc
      481 gaccgctatc cattcggctg cgccgagcaa accacgagcc gcgcgcttcc gctcctctac
      541 tatgatgagg tcgcgtcgcg cgcgcggatc agcaaagagc cgaaggcgaa agccgccatc
      601 gagaaggcca tcgcacggct ctatgagatg cagtcttcgt cgggcgcttt tggcctgtgg
      661 ggaccgtcgg gcgaagacat gtggctgacc gcctatgtga cggacttcct gacccgcgcg
      721 agggagaagg gctacgccgt tcgcgacacg aactatgaaa tctcgctcga caggctgaag
      781 aacacggtca acaacgcgcg cgatttcaag aagggcggcg aagatcttgc ctatgcgctc
      841 tatgtgctgg cgcggtccgg tcgcggcgtt ctcggcgatc tgcgctatta cgccgacacg
      901 aagatcgacg cgttttcgac gccaatcgcg cgtgcgcagc ttgcggcggg cctcgctatg
      961 ctcggcgata aggaaagggc gacgctgacc ttcgcgtctg ccttgtcggc gctcgttacg
     1021 cccgatccgc aggtgcctct cgcctatcgt gccgattacg gcacgcagct tcgagacgcc
     1081 gccgccgtgc tcgcgctgat gggcgaaagc ggagccacca cgccgcaatt acagaaggca
     1141 ttcaatatcg tcgtaaagct cagggcgcag caaaaggaga caacgacgca ggaaagcctg
     1201 tggctcctcc tcgccgcgcg cacgctcgac gcgcagaaca aagacttggc ccttgaagtg
     1261 aacggcgcgc cggtcaaggg atcgttccag acggttcttt ccggcagcga tttcggcggc
     1321 ccgatccaga tcaccaatgt gagcgcgatc ctgccaaacg ccgcctccga gacgttccaa
     1381 acaatgctga cgcctgcgca attcaccgct caggggcttg tgatcgcgaa ccgcggtccc
     1441 gaagccgtgc cggcgtcggt gctcgtgaca ggcgagggct tggagccgga gccatccgcc
     1501 gagagcgggt tcaagatcga gcgcaagacc tatgcaccag acggccgcga aatccccttc
     1561 gacaagctga agcagaacga ccgcgtggtc gtggtgctga aggtgacgga ggtcgatccg
     1621 aagcttgcgc aaatcgttgt cgaggatcgc ctgccagcgg gcttcgacat cgagaacccg
     1681 gcgctcttga aaggctcgga tctgaaagca ttctcctggc tgccttcgac ctatacgccc
     1741 gtgttttcgg ccttccggga cgaccgcttc gtcgcggcct tctctctcac cgacacaggg
     1801 cgaaaggtac cggctcagat gacgatggct tatgtcatgc gggcggtaag tcccggcgtt
     1861 tacacccatg ccggtgctcg cgtcgaggac atgtaccggc cggatcgctt tgcgcgcacc
     1921 tcgggtgcaa aggtggaaat cacggcggcg caataggcga tgatggggaa cgattcgctg
     1981 ctttccgtcc cggttacccc gaaatggctc ttgcatagag gcgcggtcgt tccatttttc
     2041 tgaaattctg gtataatgcc cgcggaattt ctgggtgggg taagtcgtga atgagtgagc
     2101 gtaaaaccca caaatggttc attcgcggcg ccctcgtttt cgctctcgtt gtgcttggtg
     2161 catcgtcgct caagcttttc gatccgctgc ccctggaggt ggcggcatca tcgtcgcggc
     2221 tcgttctcga ccgcgacggg aacctcctgc gcgccttcac cactcccgac gggcgttggc
     2281 gtcttgatgc aagacctgag gatgtaagtc cgacctatct tgcgcttctg ttcgctttcg
     2341 aggatcggcg tttctggcgg catccaggca tcgatcctct tgcgtttggg cgagccatct
     2401 tgcaagcgat tacgcacggc aaacccatat cgggcggatc gacgctgacg atgcaggtgg
     2461 cgcggttgct cagaggctct ccgacgcact ccgtctccgc caagtttcag caggttgcag
     2521 acgccgcgcg cctcgaatgg acgctcagta agcgtcaaat cctctcgctc tatctcaagc
     2581 tcgctcccta tggcggtaac ctcgaaggcg tccgcgcggc atcgcttgcc tatttcggga
     2641 aagagccgca gcggctcagc atagccgagg ccgcgctcct ggtaacgatc ccgcaatcgc
     2701 cggaagtgcg ccgcccggat cgcggatacg agtcccttgt cgatgggcgt aaccgcgtta
     2761 tccggagagc cgccgccgct ggagccataa cgcaggctga cgcgaaagcg gcgctcgcgc
     2821 aggctgtccc gcgcggacgc cgcgacttcc cggcgctggc gccacatctc tccgaacggc
     2881 tcacggtgac gttgccaaat ctgtccgcca tagcgactac gatcgaccgg aacctccagg
     2941 aagctgccga gaccatcacc cgcagaaacg cgcaaagttt cggagagaaa atctcgatcg
     3001 cggccctgat ggtaagcaac cgcacgggcg aggtgctggc tcatgtcggc tccgcaggtt
     3061 atttcgacgg ctcgcggctc ggcgcgatcg acatgacgac cgcaatccgg tcgccgggct
     3121 cggcgctgaa gccgttcatc tatggcctcg ccttcgagga tgggctcgcg catcccgaga
     3181 cgctgatcga ggacaggccg gtgcgcttcg acggctatgc gccggtcaat ttcgacaatg
     3241 tgttccatgg aacggtgacg gtcaggaccg cgcttcagct ctcgctcaac gttcccgccg
     3301 tgaaggtgct caacgaagtc agccccgtga aactggccgc gcgtttccgt gaggcaggca
     3361 tgccgtttgc cgtgccgcgc aatctcacgg tggcgctcgg cggcgcgggc cttacacttg
     3421 aaaacctgac gacgctttat gtcgggctgg ctcgcggcgg aag
//
LOCUS       DATMGX010000420         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_1597,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000420 DATMGX010000000
VERSION     DATMGX010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1819
                     /locus_tag="VNR65_06785"
     CDS             <1..1819
                     /locus_tag="VNR65_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWJ18413.1"
                     /translation="QASGSEPFVSPPPRFLHTAARMGNAPAYFVRDDGGWVGTSWNTY
                     SDEVRQAARALVSLGVKPGDSVAALGYNKPEWVIMDLAAMMIGASVAGIYFTSSAQDA
                     AYIINHAQCEIVLAEKEEHFQRIEKERAQLNVLRHVVMMKGAAADDPLQMTWEAFLAR
                     GESRFDAEVERRLHAIQPSDIGCLIYTSGTTGPPKAVQLSHGALTETAGLIQKLWNVS
                     EDDCALSYLPLAHIAERMFTVHFPLTVGNAVYFAPDIQQLAQYLKEVRPHLFFGVPRV
                     YEKIAAAAQLQIANAEGMKGKIARWAVRSGQEWHRKERMGMPPGVKTGFAKALASRLV
                     HKKAKQAIGFDRVRYFACGAAPIPEETLHFLNGLDIPVRELWGMSETCGAGTTNLPGA
                     TKVGSVGRAHPGVELKISPEGEILVKGPYIFSGYAEDPEATENALPDGWLRTGDLGRM
                     DEDGFVYITGRKKDIIITSGGKNIAPANLEMDLVALPLVEHAIVCGERRPYLTAAITL
                     NAGAVAQYASQWGLPENDPQLKEAIRDELQSGIDAINARHARVENIRRFTILPAPLSI
                     ESGDLTPTMKVKRATLLKRLQPVIDELYEQSAAEQGMAS"
BASE COUNT          446 a          590 c          624 g          394 t
ORIGIN      
        1 gcaagctagc ggaagcgaac cctttgtctc gcccccgccg cgctttttgc acacggcggc
       61 gcgcatggga aacgcaccgg cctactttgt tcgcgacgac ggaggttggg taggcacgtc
      121 ctggaacaca tacagcgacg aggtccgcca agcggcgcgc gcgcttgtct cgctgggcgt
      181 gaagccgggc gattcggtcg ccgccctcgg ctacaacaag cccgagtggg tcatcatgga
      241 tttagccgcg atgatgatcg gcgcatcagt tgcgggcatc tatttcacct cgtccgccca
      301 ggatgcggct tacatcatca atcacgctca atgcgagatc gtgctggcag agaaagagga
      361 gcacttccag cggatcgaaa aggagcgcgc gcaactgaac gtccttcgcc atgtcgtcat
      421 gatgaagggc gcggcggcgg acgatcccct gcaaatgacg tgggaggcat ttcttgcccg
      481 tggcgagagc cggttcgacg cggaagtcga gcggcgcttg cacgccattc agccgagcga
      541 tattggctgc ctcatttaca cctcgggcac gaccggtcca ccgaaagcgg tgcagttgag
      601 ccatggtgcg cttaccgaaa cggctggcct cattcaaaag ctttggaacg tgtccgagga
      661 cgactgcgcc ctctcctatc ttccgcttgc acatatcgcc gagcgaatgt ttacggtgca
      721 tttcccgcta acggtcggaa acgctgtcta cttcgcgccg gatattcagc aactcgctca
      781 gtatctcaag gaagtaagac cgcacctctt ttttggcgtg ccgcgcgttt acgaaaagat
      841 cgcggcagct gcgcagttgc aaatcgcaaa cgcggaggga atgaagggga agatcgcgcg
      901 gtgggcggtg cggtcgggcc aggagtggca ccgcaaagag cgcatgggga tgcctccggg
      961 agtcaaaacc ggtttcgcca aagcgctggc ctcaaggctt gtgcacaaga aggcaaagca
     1021 ggctattggg ttcgatcgtg ttcgctattt cgcttgcggg gcggcgccaa tcccggaaga
     1081 gacattgcat ttcttgaacg ggctcgacat ccccgtgcgc gagttatggg ggatgtccga
     1141 gacctgcggg gccgggacca cgaaccttcc cggcgcgacc aaggtcggct cggtgggacg
     1201 tgcgcatccg ggggtcgaac tcaagatcag cccggaaggc gaaattctcg tcaagggccc
     1261 ctacatattt tcaggctacg ccgaagaccc ggaggcgacg gaaaatgcgc ttcccgatgg
     1321 atggctccgc accggcgatc tcggccgcat ggacgaggat gggttcgtct atatcaccgg
     1381 gcgaaagaag gacatcatca tcacatccgg cggtaagaac atcgcacccg ccaacctcga
     1441 aatggacctt gtggcactgc ccctggtcga acatgccatc gtgtgcgggg aacgccgccc
     1501 atacctgaca gcggctatca cgctgaatgc gggcgcggtg gcgcagtacg cgtcgcaatg
     1561 gggactgccg gaaaacgatc cccagctcaa ggaagcgatc cgggatgagt tgcaatccgg
     1621 catcgacgcg atcaatgccc gccatgcgcg cgtcgagaat atccggcggt tcacgatcct
     1681 gccggcgccc ctgtcgatcg aaagcggcga tctcaccccg acaatgaaag tgaagcgcgc
     1741 aaccctttta aaaagacttc agcctgttat cgacgaactc tatgagcaaa gcgctgcgga
     1801 gcaagggatg gcctcctaac ttctatcaag gcgacaggcg gtcgatatta tccagatcgt
     1861 ctccacaaca tgcaggttgg tgcgccccat ttcacgttga ccgtttttgt cttcgatatt
     1921 tgggcctgat tttataacgc gcccacgttt ccaggtggcg gaaaaataga tagatcgtgc
     1981 gcgaggcgtc atcgcgaaag gttgtcaata tatcaaaaaa tctcgagtct gctgataagg
     2041 agtccgagcg gaat
//
LOCUS       DATMGX010000421         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_425967, whole genome shotgun sequence.
ACCESSION   DATMGX010000421 DATMGX010000000
VERSION     DATMGX010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425967"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            674..1993
                     /gene="ltrA"
                     /locus_tag="VNR65_06790"
     CDS             674..1993
                     /gene="ltrA"
                     /locus_tag="VNR65_06790"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HWJ18414.1"
                     /translation="MSLETPEKIRSLQRKLYVKAKAEPEFRFYLLYDKVYREDILEHA
                     YKLAKASRGAPGVDGVTFAQIEEAGVKEWLSGLRQDLREKRYEPQPVRRVMIPKPGGG
                     ERPLGIPTIRDRVVQTAVKIVIEPVLEADLSPYMFGYRPRKSALDAIKMVQGMLKKGY
                     ADVVDADLSKYFDAIPHSELLKSIAVRISDRTILRLIKMWLKAPVEERDKNGGRRMSG
                     GKSAKTGTPQGGVISPLLANRYMNRFLRYWAKTDQPRRLRARIVSYADDFVILSRGRA
                     AEALDWTRRAITRLGLTLNEAKTKLKDARRERFEFLGYSFGPHWHQRDGKSYLGASPS
                     PKSVARLRQNVRDHLKTFHNRPWPDIRDGLNAILRGWDGYFCYGTRWTAHRAIDAHVV
                     QSLRGFLVRRHKAPSRGVRRFSGEAIFGDMGIFSLVANKAKPPIALT"
BASE COUNT          477 a          599 c          705 g          398 t
ORIGIN      
        1 cctgcgagag aactctattc atttcaatgg catatcggaa ttatttcctg tcacatgaaa
       61 ggcgcggcgc tcttcttttg cgccagagta aacgcctctt taagcataat ggtgaccgga
      121 aataaatccg gcgcgctcct gttaaccact ttcgattaac gttcggcgcc gcgaacgaac
      181 ggtttacgtg cgccacgaag gcgcggatga ccagtggagg aaggttccac ccggagaatg
      241 gttggttagc tccggtagct gacaggcggc ttggcgaggc aactggccgg gcggaagccc
      301 tgtgacaaag gccgccttgg gcggtcgagc aacctgacgg gccgtaacgc gagtgaactc
      361 tgagcaggcc tcgaaagcgt taatgcggat gctgaccctc cgtaaacaag gggaaagctt
      421 gcactgacaa ggaagcaacc aacaagcgca cttgtcagat ccgccggggt agtggagacg
      481 gcacgtgggg acggtcatcc gggtaacgtg ggagacccgt gcggccagga tcgcaacctg
      541 aacgccagaa gatggcgggc cgtccgggag tcggacaggc gcatagtact gatgaagccg
      601 ggtaactccg gtggagggaa ggcgcctgac ttctggcacg cttttgaaga aggcaaggat
      661 caggggattg gcgatgagcc ttgaaacgcc ggaaaagatc aggagtcttc agagaaagct
      721 ctacgtcaaa gcgaaggcgg agccggagtt ccgcttctac cttctgtacg acaaggtcta
      781 tcgcgaagac atcctggagc acgcctacaa gctcgccaag gccagccgcg gcgcgccggg
      841 cgtggacggg gtcacattcg cgcagatcga ggaggcaggg gtgaaggagt ggctctccgg
      901 cctgagacaa gacctgcggg agaaacggta cgaaccacaa ccggtgcgga gggtgatgat
      961 cccaaagcct ggtggcggcg aacggcctct cggcatccca acaatacggg accgggtggt
     1021 gcagacagcc gtcaagattg tgattgaacc ggttctagag gcggacctgt ctccgtatat
     1081 gtttggctat cgtcctcgga agagcgcgct cgacgccatc aaaatggtgc agggcatgct
     1141 caaaaaggga tacgccgatg tggtggacgc tgacttgtct aagtatttcg atgccattcc
     1201 tcattccgaa cttctcaagt caatcgccgt gcgcatctcg gaccggacca ttctccggct
     1261 catcaagatg tggctaaagg ctccagtcga ggaacgggat aagaatggag ggaggcgcat
     1321 gagcggcggc aaaagcgcga agacaggaac cccgcaaggg ggagtcattt ctccgcttct
     1381 ggccaaccgt tacatgaatc gcttcctgag atactgggca aagacggacc agccgcgccg
     1441 cttgcgcgcg cggattgttt cgtatgccga tgactttgtc attttaagtc gtgggcgggc
     1501 tgccgaggcg ctggattgga cgcgccgtgc gataacccgg cttgggctga ccttgaacga
     1561 ggccaagacc aagctcaagg atgcacggcg ggaacggttc gagttcttag gctacagctt
     1621 cggcccgcac tggcatcagc gggatggcaa atcgtatctg ggggcgagcc cgtcgccgaa
     1681 gagcgtcgcc cggctcaggc aaaatgttcg cgatcatctc aaaacgttcc acaaccggcc
     1741 ctggccggac atccgggacg ggctgaacgc gatcctgcgc ggctgggacg gctacttctg
     1801 ctacggaacc cgctggacag cgcaccgagc cattgacgct cacgtcgtgc aatccttgcg
     1861 gggctttctt gtcagacggc acaaagcgcc atcgcgaggc gttcgtcgct tcagcggtga
     1921 ggcgatcttc ggagacatgg gcattttcag cctggtcgcg aacaaggcta agccgccgat
     1981 agcgttgacg tgaagccagt cggaaagccg gatgctggaa atcggcacgt ccggtttgat
     2041 gagcggggag gggaaacggg gcgttgccgt atggcccaag ctaccgcgcc tctcctcgac
     2101 tctacccatc atggcagcgg cgcccgcctt gagcgggacg atggctacgg gctcgcgctt
     2161 ggtctccgcc tcgctacct
//
LOCUS       DATMGX010000422         2248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_396346, whole genome shotgun sequence.
ACCESSION   DATMGX010000422 DATMGX010000000
VERSION     DATMGX010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396346"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VNR65_06795"
     CDS             <1..932
                     /locus_tag="VNR65_06795"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HWJ18415.1"
                     /translation="VRTYLVFGSDRSNFGQLEAHFAANYVRYIAQFVAWLAVFSIALT
                     ALGHKAREFIPSFIVLYVLSVIIFALGQWDKANTYNLEPPLVALVLGLLLSNVVGLPR
                     WLDSGFRVEFYIKTGIVLLGATLPFTLIIWAGPVAILQASIVSIVTFLVIYWVGLKLG
                     LDRRLAATLGAGGAVCGVSAAIAISGAVGGKKEDAPIAITVVIIWAIAMIFVLPFVAQ
                     YLHLPAGVGGAWIGTSEFADAAGFAAADTYGHIASSGAVAGTSDQAVFAYTLIKVVGR
                     DVWIGIWAFVLAIIATTQWERTETGRKPDAAEI"
     gene            complement(1130..1333)
                     /locus_tag="VNR65_06800"
     CDS             complement(1130..1333)
                     /locus_tag="VNR65_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18416.1"
                     /translation="MNENLLIGVVSLGIAAGLLFLGWPDKNGESPRFLQFEASLVLYP
                     SIIMVFLAAGFAELITGLLRMPH"
     gene            1628..1897
                     /locus_tag="VNR65_06805"
     CDS             1628..1897
                     /locus_tag="VNR65_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18417.1"
                     /translation="MPHRRSRANASPLLRGRFRQRYDEIEAKRAELVARLRRLGTNGK
                     QHPGYKRALRLLNNTFRKSKLPQRLAVLQAAAWLIDVLEQTTMML"
BASE COUNT          480 a          642 c          631 g          495 t
ORIGIN      
        1 cggtacgcac gtatctagtt tttggaagcg acaggtctaa tttcggtcag ctagaagcgc
       61 atttcgccgc caattacgtt cgctacatcg cgcaattcgt cgcgtggctg gccgtctttt
      121 caatcgcact cactgcgcta gggcataagg cgcgcgaatt catcccgtct ttcatcgtcc
      181 tttatgtgct gtccgtcatc atctttgcgc tcggccagtg ggacaaggcg aacacctata
      241 accttgagcc gccactggta gctctggtgc tcggcctgct gctttccaat gtcgtcggct
      301 tgccgcgctg gctcgattcc ggtttccgcg tcgaattcta catcaagact ggcatagtgc
      361 tgctgggtgc caccctgccc ttcacgctca tcatctgggc cggaccggtg gccatcctgc
      421 aagcctcgat cgtctcaatc gtcacgttcc tagtgatcta ttgggttggc ctgaagctgg
      481 gtcttgaccg gcggctcgcc gccacattgg gcgccggcgg cgcggtctgc ggcgtctctg
      541 cagccattgc gatctcaggc gcggtcggcg gtaagaaaga ggacgcgccg atcgcaatca
      601 ccgtggtcat catctgggcg attgcgatga tctttgtgct gccgtttgtc gcgcaatatc
      661 tacatctgcc ggcgggtgtc ggcggtgcct ggatcggtac ctccgaattt gccgatgctg
      721 ccggcttcgc tgccgcggat acgtacgggc atatcgcgag cagtggcgcc gttgccggca
      781 ccagcgatca ggcggtgttt gcctataccc tgatcaaggt ggtcggccgc gacgtttgga
      841 tcggcatctg ggctttcgtg ctggcgatca ttgccacgac gcaatgggag cgaaccgaga
      901 ctggccgaaa gcccgatgcg gccgagatct gatgatccag aaaggttacg tgattctggc
      961 agcctatcaa tgcggatcag gtttttggta cacacagcgg ttattgcatt ggtcgatgcg
     1021 cgctcaacgc ccatcggtcg ggtgacgcgg tgaggcagtc gtagccgatc accaaataaa
     1081 cgtataggcc gctggaaccc tgcgccgacc gccaactgat gaagcggcct tagtgcggca
     1141 tgcgtagaag ccctgtaatc agctcggcaa agccggccgc taagaagacc ataataatag
     1201 acggatataa aaccaatgac gcctcgaatt gcaggaagcg cgggctttcg ccatttttat
     1261 ctggccagcc gaggaaaaga agaccagcag caatgcctag cgaaaccaca ccgatcagga
     1321 ggttttcgtt catctgattt cctcccatct ctgctgacaa aacagaatgt ttatgcaata
     1381 tttatactat ggatcctgac aacaattcaa tggtggcaat gcgttgccct agctgcaaac
     1441 agggctcgat ggtgtcctta accggacagt ccacggcgat aggacgatga ccatttattg
     1501 gcgctgcccg gcctgctccc atgtccaagt cgaggaagcg tcgaccgaca gatgtcctca
     1561 ctattgattg agcagttgat tccaattact agagagaagc gtctcggcca gataacgtta
     1621 accgccaatg cctcatcgcc gctcacgcgc caatgcgtcg ccgctcctgc gcggccgctt
     1681 ccgccagcgc tacgacgaga tcgaagcgaa acgcgccgag ctggtcgcgc gtctacgcag
     1741 gctcggtacc aatgggaaac agcatcccgg ctacaagcgc gcgctgaggt tgctcaataa
     1801 tactttccgc aaatcgaagt tgccgcaacg gctagcggta ctgcaggccg cggcctggct
     1861 gatcgacgtg ctggaacaga ccacaatgat gctgtaacca gcgcacctaa gcggtatttt
     1921 ccgcggccgt gccgctgcag gttttaattt gtcttttcaa acacttatca gctaattatg
     1981 agacttccgg cgtaagacct gaggtgcaca gacgacgtta ttgcgccggg cataccttaa
     2041 tgcggggacg gctttagcga acaggtgaat gcgcacgaag gcggccccaa caaaaaaaac
     2101 aaatcagcac acagaatggg aaaacgccta tgacaacaac gaccgctaat caggttcgca
     2161 cgggcgggat gaccgccgaa gagcggaagg tcatcttcgc ctcttcgctc ggcactgtgc
     2221 tggagtggta tgacttctac atctacgg
//
LOCUS       DATMGX010000423         5759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_106826, whole genome shotgun sequence.
ACCESSION   DATMGX010000423 DATMGX010000000
VERSION     DATMGX010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106826"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VNR65_06810"
     CDS             <1..717
                     /locus_tag="VNR65_06810"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HWJ18418.1"
                     /translation="SLMKAESERIQTKLFNTQKSFGTSSMTGSWLRQESVDGPTILHA
                     KSSADRGLTLGGITLLQFDLNNRFYEQIQANKAELRQGYWRLENATVQSSGEAIKRYK
                     EYFISTYLSPIQIMNSLGSIETLSFWELPGFIDFAKKAGIPTEQYELQYQLFLSRPIL
                     MAAMVMIAATCSLKAFRFGRIQTMVLTGLTGGFGFFIFSELSRKVGASGVVPVTVAAW
                     APALTALLLAATVLLHQEDG"
     gene            887..3067
                     /locus_tag="VNR65_06815"
     CDS             887..3067
                     /locus_tag="VNR65_06815"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HWJ18419.1"
                     /translation="MAKQVSPDKASPLLLQADDLVYDNRNSRVIARGNVEIYYNDNVL
                     LADEIVYDKSANTLSAMGNVRLKDSDGSVVNEERLTLSADFRDGFIRSLRALTQDDTR
                     VAASNAYRKGDKTIFENGVVTACKPCEANPDRPPIWRVKSERVVEDKTDQNVYYENSV
                     FEVFGIPVAWVPWFYTPDPSVKQRSGFLAPSYAVDSSLGYVVAIPYYWGISPNYDLTL
                     TPEFTSKAGYFMQADWRQRLWNGAYEVKLDGAFNDQAQDFFGDRNWRGSVETKGNFAL
                     NSRWTVGWNAILESDDTFRRFYRLDDIYATERVSSVFLTGMGDRNYFNMTVAQYGNLT
                     GNIYNYDTNTFHQTVTATSYPVIDYNYIHNKPVLGGELSFDLNALALNVNDPAGLLPL
                     AYRGTMNHVVSQAEWRRTLTDDIGERFTPFIIGRADLYNVSNFRDIDGVTGTSDTFTR
                     QMIGGGLDYRYPFVAHTESASHVIEPVAQIIARGGGSNSKVPNEDSQSLVFDDTLLFD
                     INKFSGYDRIENDTRANVGLQYTYQNYSGISLRAVGGESVHLAGSNPYDPKTGLGTDA
                     SDYVFGGYVDYMNRFRLLTQFRVSEQNLAFSSQNYSLQTKLGFFQGALSYVSVAAQPQ
                     LGFLTGRDEIAGFSAVKLNDEWTVFGDLRYDIELSQWVRNSAGIQYADECFILSVTYQ
                     QSYVSYLDIVPSTSVLVRVGIKGFGQQTTPSSIGDLSPEAAVFR"
     gene            3171..4277
                     /locus_tag="VNR65_06820"
     CDS             3171..4277
                     /locus_tag="VNR65_06820"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HWJ18420.1"
                     /translation="MRIEILYRLIFLAAGGIFFALLTGIPEANAQNVVVLVNDEPITS
                     GDVAQRIRWNARTNGFGDRMKAMLTGDAIKERFRQAMIAASPHSQAEAEQAAEKIKKQ
                     LLEDAKNRVLSDGGGTSRKAAIDSLVEDKLKLQAAKKLDIKVSDSEVEQVLAQRAGGA
                     AEGKKPDLSEFYKQFETDGITRKTIQDIIRAQLAWRDVIRRQYGPRLASLFSAMPNTD
                     EKPSESDIQFDVRVLRLSVSGSDQKAISQRIMEAENLKQKFTSCAQLPNEAKLVSDAT
                     VRSIDKAKLASFPKDVQPLIQKASEGQMTPPVLVGSAIESYAICRKGLAAKSAKAAAE
                     QKPDVRQQEYERFSRRYLQDLKRSASIDYRGKTD"
     gene            4286..5365
                     /gene="pdxA"
                     /locus_tag="VNR65_06825"
     CDS             4286..5365
                     /gene="pdxA"
                     /locus_tag="VNR65_06825"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF003699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWJ18421.1"
                     /translation="MHGRDESSSRPGSLRKPLAVTMGDPAGIGPDVTILAWLRRNADR
                     IPAFFVVGDPTVFVDRVGLIDNRDSILIQEIQSPEQAAELFPNALPVLPLNMPLGPVI
                     AGAPSAAHAPAIIDSIEEAVAFTVAGRASAIVTNPIAKFVLLEAGFAHAGHTEFLAEL
                     AVRHGSPAVTPVMMMASKRLKAVPVTVHIALKNVPSAITKDRIMTVASITSSALSHSF
                     GIPQPRLAVCGVNPHAGENGALGREEIEVIAPAIEALNAQGIHATGPHSADTLFHDAA
                     REHYDAVLAMYHDQALIPFKTLSFADGVNVTLGLPFIRTSPDHGTAFSLAGTGHADPA
                     SFIEALHLAGRMVRSAAAAPSASQP"
     gene            5362..>5759
                     /locus_tag="VNR65_06830"
     CDS             5362..>5759
                     /locus_tag="VNR65_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HWJ18422.1"
                     /translation="MTSADSIASLPPLREVIKEAALSARKSLGQNFILDLNVTRRIAR
                     AAGPLENVTVLEIGPGPGGLTRALLLEGAAGVVTIERDERFRPALSQIEARSGGRLKA
                     LFMDALKADYAAIAAETGARRIVANLPYNIA"
BASE COUNT         1271 a         1655 c         1578 g         1255 t
ORIGIN      
        1 tcgctgatga aagccgaatc agagcgcatc caaaccaagc tcttcaacac tcagaagtcc
       61 ttcggaactt cgagtatgac cggctcgtgg ttaaggcagg agagcgtgga tggcccgacg
      121 attttgcacg caaaatcatc cgccgacagg ggtttgacgc ttggcggcat aaccctgttg
      181 caatttgatc tcaacaatag attttatgaa cagattcaag caaacaaggc ggaacttagg
      241 caagggtatt ggcgcctgga gaacgcgaca gtacaaagct ctggcgaggc cattaaacga
      301 tacaaagagt atttcataag tacatatctc agccctatac agattatgaa tagtctgggc
      361 tctatagaaa ctctgtcatt ttgggagctt cctggattta ttgattttgc aaaaaaggcc
      421 ggaataccaa cagagcagta tgagcttcaa taccagcttt tcctgtcgag acctattctt
      481 atggcggcga tggtgatgat tgccgcaacc tgctcgctga aagcctttcg ttttggcagg
      541 attcaaacta tggttctcac gggtttaacc ggggggtttg gctttttcat attttcagaa
      601 ctatcgcgta aagtcggtgc gtcgggggtg gtgccggtaa cagttgcggc ctgggcgcct
      661 gccctaacag cgctgctatt ggcggcgact gtgctgctcc atcaggagga tgggtgagtt
      721 tgggggggcc gaggcctttt gagggtgacg gttgacagtg tggaagcggc gaacaatgct
      781 tagggggctt gtggcagtcc tggcatgctt ggcatgctgc gggggcagcg tgcgcgcgca
      841 gcaatccact gttacggctc tacagtccca cgcagccgat caggttgtgg ctaagcaggt
      901 ttctcccgac aaagcgtcac cgctccttct ccaagccgat gacctcgttt acgacaaccg
      961 caacagtcgt gtcatcgccc ggggcaacgt cgagatttat tacaacgaca atgtcctgct
     1021 cgccgacgag atcgtttacg acaaatcggc gaacaccttg agcgccatgg gtaatgttcg
     1081 cctgaaggat tcagacggct cggtcgtcaa tgaggaacgg ctgaccttga gcgccgattt
     1141 tcgtgacggc ttcatccgct ccctgcgcgc tctcacgcag gatgacacgc gggttgcggc
     1201 ttccaacgcc taccggaagg gtgacaaaac gatcttcgag aacggcgtcg ttaccgcttg
     1261 caagccctgc gaagccaacc cggatcggcc tccgatttgg cgcgtcaagt cggagcgcgt
     1321 ggtcgaggac aagacagacc agaatgttta ctatgaaaac tccgtgttcg aggttttcgg
     1381 aatacctgtc gcgtgggttc cctggttcta tacgccggac ccttcggtca agcagcgatc
     1441 cggcttcctg gcgccgtcct atgctgtcga cagcagtctt ggatacgttg ttgcgattcc
     1501 ctattattgg gggatttcgc ccaattacga tcttacgctt acgccggaat tcacatcgaa
     1561 ggccggttac ttcatgcagg cagactggcg gcagcgtctt tggaacggcg cttatgaagt
     1621 gaagcttgac ggagccttca atgatcaggc tcaggacttc ttcggtgaca gaaattggcg
     1681 cggcagtgtg gaaacgaagg gtaacttcgc cctgaactcc cgctggactg tcggttggaa
     1741 cgccattctc gaaagcgatg atacatttcg cagattttac aggctcgacg atatatatgc
     1801 gacggagcga gtatcttctg tctttctaac gggaatggga gacagaaatt atttcaatat
     1861 gacagttgcc caatacggca atctgaccgg aaacatctac aattacgaca cgaatacatt
     1921 tcaccagacc gttaccgcga cgtcttaccc tgtcattgac tacaactata tccataataa
     1981 gcccgttctt ggcggcgaac tcagctttga cctgaacgcc cttgccctga acgtgaacga
     2041 tcctgccggc ctgctccccc ttgcctatcg cggaacgatg aatcatgtcg tttcacaggc
     2101 tgaatggcga cgcacgttga ccgacgacat tggcgagcgg ttcaccccat tcatcatcgg
     2161 gcgcgccgat ctttacaatg tgtctaattt cagggatata gacggcgtca caggcacgag
     2221 cgacactttc acgcggcaga tgatcggggg tggcctggac tatcgctatc ccttcgtcgc
     2281 tcacacagaa agtgcatcac acgtcatcga gcctgtggct cagattatcg cccgcggcgg
     2341 cggctccaac tcaaaagtcc ccaacgagga ctcgcaaagc cttgtctttg acgacaccct
     2401 ccttttcgac atcaacaagt tctccggcta cgaccgtatc gagaacgaca cgagggccaa
     2461 tgtcggcctg cagtacacct accagaatta tagcggcatc agcttgaggg ctgtcggtgg
     2521 cgagagcgtg catctggctg gcagcaatcc ctatgacccg aaaaccgggc tcggcacaga
     2581 tgcctcggat tacgtcttcg gcggctatgt ggattatatg aaccggttcc gcctcctgac
     2641 ccagttccgc gtcagcgagc agaacctcgc attttcgagt cagaattaca gcctccagac
     2701 caagctgggc ttcttccagg gagcactcag ctatgtttcc gtcgccgcgc agccgcagct
     2761 tggcttcctt accggtcgcg atgaaattgc cggattttct gctgtcaagc tgaatgacga
     2821 gtggaccgta ttcggagacc ttcgctacga tatcgaactc agccagtggg tacggaatag
     2881 tgcaggcatt caatatgccg atgaatgctt cattctttcc gttacttacc agcaaagcta
     2941 cgtctcatat ctcgacattg tcccgagtac ctcggtcctg gtccgcgttg gcatcaaggg
     3001 cttcggccag cagacgaccc caagctcgat cggcgatctc tcacccgaag cggccgtctt
     3061 ccgttaaagg gtgtggaact gaatttgctc cttgtgagtg cgtcgttgcc acctggtgat
     3121 ttcggctgac gcggcaaact cttgccgcaa atgatatcca agctcccgct atgcgaatcg
     3181 agattctata tcgactgata tttctggcgg ccggcggcat attttttgcg ctcctgacag
     3241 gcatccccga agccaacgct caaaatgttg tcgtgctggt caatgacgag ccgataacct
     3301 ccggcgatgt cgcgcaaaga atacgctgga acgcgcggac gaacggcttt ggcgatcgca
     3361 tgaaagccat gctcaccgga gacgccataa aggaacgttt ccggcaggcg atgattgcgg
     3421 cgagccctca ttctcaggcc gaggctgaac aagccgccga gaaaatcaaa aagcagcttc
     3481 ttgaagatgc caagaaccgg gtgctctcgg acggtggcgg cacctcccgc aaggcggcaa
     3541 tcgattcctt ggtcgaggat aagcttaaac tgcaagcggc gaaaaagctg gacatcaaag
     3601 tctcggattc cgaggtcgaa caggttcttg cgcaaagggc tggaggcgca gccgaaggca
     3661 agaaacctga tctatcagag ttctacaaac agttcgagac cgacggcatc acccggaaaa
     3721 ccatccagga tatcattcgt gcccagcttg cctggcgcga tgtcatccgc aggcagtacg
     3781 ggccgcgatt ggcgtctctt ttctctgcaa tgccgaacac tgacgagaag ccttctgaaa
     3841 gcgacattca attcgatgtt cgcgtcttgc gcctcagcgt ctccggctcc gatcagaagg
     3901 ccattagcca acgcatcatg gaagctgaga atttgaaaca aaaattcacc tcctgtgcgc
     3961 agctcccgaa cgaagcgaaa ctcgtttccg atgcgacggt aagatcaatc gacaaagcga
     4021 agcttgcctc cttcccgaag gatgtccaac ctttgattca gaaagcctcg gaaggacaaa
     4081 tgacgccgcc ggttcttgtc ggcagcgcca ttgaatccta tgctatatgt aggaaagggc
     4141 ttgctgcaaa gtccgccaaa gctgcagcgg agcaaaagcc tgacgtccgt caacaggaat
     4201 acgagcgctt ttcgcggcgc tatctgcaag acttgaagcg atccgcatcg atcgactacc
     4261 ggggaaagac agactgataa catctttgca tggccgggat gagtcatcct cgcggcctgg
     4321 gtcccttcgg aaaccgcttg ctgtcacaat gggtgaccct gcgggcatcg gccccgacgt
     4381 tacgatctta gcctggctgc gccgaaatgc ggatcgtatc cccgcatttt ttgtcgtcgg
     4441 cgatccgacc gtcttcgtcg atcgtgtcgg gttgatcgat aaccgcgact cgattctcat
     4501 ccaggagata caaagcccgg agcaagcggc agagctcttc ccgaatgccc tgcccgtgtt
     4561 gcccctcaat atgcctctcg gcccagtgat agcgggcgca cccagtgctg cgcacgcccc
     4621 tgcgatcatc gactccatag aggaagccgt tgccttcacc gtcgctgggc gggcctcggc
     4681 catcgtcacg aatccgattg ccaaatttgt gctgcttgaa gcgggctttg cccatgcagg
     4741 gcacacggag tttctggcag agcttgccgt acggcatggc agccccgctg tgacccctgt
     4801 catgatgatg gcatcgaagc gtctcaaagc cgtccctgtc acggtccata tcgcattgaa
     4861 aaacgtgccg tccgcgataa ccaaggaccg catcatgacg gttgcttcga taaccagcag
     4921 cgccctctcg cactctttcg gaattcccca gcccaggctc gcggtttgcg gcgtaaatcc
     4981 acacgccgga gaaaatggag cgcttggccg cgaagaaatc gaggtgatcg cgcccgcgat
     5041 cgaggcactc aatgctcaag gtatccacgc aaccgggccg cattcggcgg ataccctgtt
     5101 tcacgacgcc gcgcgcgagc actatgacgc cgttctggcc atgtaccacg accaggcgct
     5161 catcccgttc aagacgctct ctttcgcgga tggcgtgaac gtcacgcttg gcctgccctt
     5221 cattcggaca tcgcccgatc atggcaccgc cttttcgctt gccgggaccg ggcatgccga
     5281 tcccgcaagc tttatcgaag cgctccacct cgcgggccgc atggtgagga gcgcggcggc
     5341 ggctccatca gcgtcgcagc cgtgacctcg gctgattcca tagcgtcact gccgcccctt
     5401 cgcgaggtca tcaaggaggc cgcgctttcg gccaggaagt cgctcggcca gaacttcatc
     5461 ctcgacctga atgtgacccg gcgaatcgct cgcgccgccg gcccacttga aaacgtcacg
     5521 gttcttgaaa tcgggccagg gccgggcggc ttgacacgcg cgctccttct tgaaggcgcc
     5581 gccggagttg ttaccatcga gcgcgacgag cgattccggc cagcgctttc gcagatcgag
     5641 gcaagaagcg ggggccggct caaggcattg ttcatggacg cgttgaaggc cgactatgca
     5701 gccatcgccg ccgagacagg ggcacgccgg attgtcgcca atctcccata caatatcgc
//
LOCUS       DATMGX010000424         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_138065, whole genome shotgun sequence.
ACCESSION   DATMGX010000424 DATMGX010000000
VERSION     DATMGX010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138065"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VNR65_06835"
     CDS             complement(<1..634)
                     /locus_tag="VNR65_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWJ18423.1"
                     /translation="MVRPQATPAQTADEAPIEAFLHMMRAERGASSNTISAYAADLRE
                     MSAFLAADRTTLLSCSRQNLEAFFADRAESGLAANSAARKLSCLKRLMQFLVAEGLRA
                     EDPARLIDGVKGRRSLPATLSIAEVDLLLDAAHVAPAAATPGALRRALRLACLLELLY
                     GTGLRVSELVGLPRSALRGDRMMLTVKGKGGRERILPLNQKARDILDKWLE"
     gene            896..1510
                     /locus_tag="VNR65_06840"
     CDS             896..1510
                     /locus_tag="VNR65_06840"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF010552.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWJ18424.1"
                     /translation="MTTENDSGSSRPLARSVRRALQKRSVILVGMMGSGKSSIGRRLA
                     ATLELPFVDADAEIEQAAGMTIEDVFTVHGEPYFREGEERVIRRILQGGQQVLSTGGG
                     AVVSQQTRAAIAGSGISIWLDAPVDLLLQRVLRRDNRPLLKTENPRAVLERLLFERQP
                     YYREADLKVESRDVPHEAVVDDILALLSSYLASQEQGGAPTQQD"
     gene            1519..2643
                     /gene="aroB"
                     /locus_tag="VNR65_06845"
     CDS             1519..2643
                     /gene="aroB"
                     /locus_tag="VNR65_06845"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWJ18425.1"
                     /translation="MAANVAARVDVKLGDRSYPILIGSGLLASAPDHLAQALPDARFA
                     IVADDSVRAYAEVIERGLSVKSSLLGAPIYVPSGEASKSFPELERVCTSLLDLGIERN
                     HAVIAVGGGVLGDLAGFAAASVKRGVRLVQVPTTLLSQVDSSVGGKTGVDTPHGKNLV
                     GAFHQPSLVLTDLDTLKTLPPREFRSGYAEVVKYGALGDAVFFRWLEQNADAVFDQER
                     DDLAYAIETSCQMKAEIVSRDEREAGDRALLNLGHTFGHAIEAWAGYSGEVLHGEAIA
                     LGLVLAGEFSFKQGFCGSQVASRLKTHLGEYGLPVSFADLRKQVGRAPGLDELMSFME
                     QDKKVKDGEMTLVLFRGMGDTFVARNVARSGIRAFLESQL"
BASE COUNT          543 a          859 c          933 g          562 t
ORIGIN      
        1 cctcaagcca cttgtcgagg atatcgcggg ccttctggtt cagcggcaga atgcgttcgc
       61 gcccgccctt gcccttgacc gtcagcatca tccggtcgcc gcgaagggcg ctgcgaggca
      121 ggcccaccag ttccgagacg cgcaatcccg ttccatagag gagttcgagc agacaggcga
      181 gacgcagcgc acgccgcagc gcgcccggag ttgccgccgc gggcgcaaca tgggcggcat
      241 cgagaagaag gtccacctcg gcaatcgaca acgtcgcggg caggctccgc ctccctttca
      301 cgccatcaat gagccgggcc ggatcctcgg cgcgcaggcc ttccgcgacg agaaactgca
      361 tcaggcgctt gagacaggaa agcttacggg ccgcggaatt cgctgcaagc ccgctctcgg
      421 cgcgatccgc gaaaaaggcc tcaaggttct gacgcgaaca ggaaagaagc gttgtgcgat
      481 ctgcggcaag gaaggcggac atctcccgca aatcggcggc gtaggctgaa atcgtattgg
      541 aagacgcgcc ccgctctgcc cgcatcatat gcaggaaagc ctcgataggg gcctcgtcag
      601 cggtctgagc gggagtggct tgaggccgca ccattctatt ttatctttgc gtctcggatc
      661 gactttgtca tttcgtgtgg ctgcggctca aagaagtttg cgagcacgaa catggagccg
      721 taaaacaacc caacaagaat accaacgatc atcaaaaatc gtaaaagtgt cggcatgaaa
      781 gcctccaggc cggttccttt gtaaatgggc tgccgttctt cgcgcccgtt gtcaaggatg
      841 ggatgtaggc ataagttgag ggattgcgat ccatacgagc tcgaagcgtg caacgatgac
      901 gacggagaac gacagcggca gtagcagacc tctggcgcgg tccgtgcggc gcgcgcttca
      961 aaaacgcagc gttattcttg tcggcatgat ggggtcgggc aagtcttcga taggccgccg
     1021 cctcgccgcg acgctcgaac tgccttttgt cgatgcggat gccgaaatcg agcaggccgc
     1081 aggaatgacg attgaggacg tgttcacggt gcacggggag ccctatttcc gcgaagggga
     1141 agagcgcgtc atccggcgaa tcctccaggg cggtcagcag gtgctttcga ccggaggagg
     1201 cgctgtggtc tcacaacaga cacgcgcagc aatcgccggg agcggcatct cgatctggct
     1261 tgacgccccg gtggatcttc tgctgcagcg tgtcttgcgc cgcgacaatc gcccgcttct
     1321 caaaaccgaa aatccgagag cggttctgga acggcttctc ttcgagcgcc agccttacta
     1381 ccgtgaggcc gatctgaaag ttgaaagccg cgatgtgccc catgaggcgg tcgtggatga
     1441 tatccttgcg cttctgtcct cgtatcttgc gtcgcaggaa cagggtgggg cgccgacaca
     1501 acaagactag gagggattat ggcagcgaat gtggcggctc gtgtggatgt caaactcggg
     1561 gatcggagct accccattct cataggaagc ggcctcctgg cctcggctcc cgatcatctt
     1621 gcgcaagctt tgccggatgc gcgctttgcg attgtggcgg acgattcggt gcgagcctat
     1681 gcggaggtga ttgaacgcgg cttgtccgtg aagagttcac ttcttggcgc gccgatctac
     1741 gtaccctcgg gcgaggcgtc caaaagcttc cccgagcttg aacgcgtgtg cacctctctt
     1801 ctcgatctcg gcatcgagcg caaccacgcc gtgatcgccg tcggcggtgg ggtgctcgga
     1861 gatctggccg ggtttgcggc cgccagcgtc aagcgcggcg tgaggctcgt tcaggtgccg
     1921 acgacgctgc tttcgcaggt ggattcttcc gtcggcggga agacgggcgt cgacacgccc
     1981 cacggcaaga atttggtcgg ggccttccat cagccgtcgc ttgtgttgac cgacctcgac
     2041 acgctcaaga cattgccgcc gcgcgagttc cggtctggct acgccgaggt cgtgaaatat
     2101 ggcgcgctgg gtgacgccgt cttcttccgt tggctcgaac agaatgccga cgcggtgttc
     2161 gatcaggaac gcgatgatct ggcctatgcc atcgagacaa gctgccagat gaaggcggaa
     2221 atcgtgtccc gcgacgagcg tgaggcaggc gaccgcgcgc tcttgaatct cgggcacacc
     2281 tttggccatg cgatcgaggc ctgggccggc tattcgggcg aggtgctgca tggcgaggcc
     2341 atcgcgctcg gcctggtgct cgcgggcgag ttctccttca agcagggttt ttgcggatcg
     2401 caagtggcct cccgattgaa gacacacctc ggtgaatacg gcctgccggt gagcttcgcc
     2461 gacctgcgca agcaggtggg ccgcgcgccg ggcctcgacg agctgatgtc cttcatggag
     2521 caggacaaga aggtcaagga cggcgagatg acgctcgtcc tcttccgtgg aatgggggat
     2581 acgtttgtcg cccggaacgt ggcgagatcc ggcattcgcg cctttctgga gtcccagctc
     2641 taaacgggca gccgcgcggc ctcctttgtg tgcggcgcgg ccgagctgtc gtttgtcagc
     2701 ctctggcaaa cctttccaat tgccgggccg ccttgagctt ccgagaagcc tcatcgcggc
     2761 ccgcccattc tcggcaacga cgcgatggcg ccggtcttgc aagcgtgctg aatagttgcg
     2821 agttgacccc gcgcaccagg ccgaaagctg atgttccaat gggatttttc cgctgcgcgg
     2881 cgactggagc ccgattt
//
LOCUS       DATMGX010000425         2990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_320512, whole genome shotgun sequence.
ACCESSION   DATMGX010000425 DATMGX010000000
VERSION     DATMGX010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320512"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /gene="otsB"
                     /locus_tag="VNR65_06850"
     CDS             <1..747
                     /gene="otsB"
                     /locus_tag="VNR65_06850"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWJ18426.1"
                     /translation="AVLPILRDASAYSFFFDFDGTLTEIAETPGAVVVERRAREALEM
                     LFESASGAVAIVTGREIDSIDKFLAPLKLPVAGVHGYERRNGETTVSATAAEESAARA
                     VAKVFTAFVDHNPGLLLEKKRGALALHYRLRPELETFCLSLAEDLSCRFADVVLTRGK
                     MVVEMRLHTATKGTAINDFLQEKPFRDRLPFFAGDDVTDEDAFETVNTLGGITVKVGA
                     GETAARARAASVDEFLTWLLATAQCSKELG"
     gene            750..2549
                     /locus_tag="VNR65_06855"
     CDS             750..2549
                     /locus_tag="VNR65_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWJ18427.1"
                     /translation="MANNLDLGLIGNCSISALVDKEARIVWCCLPRFDGDPVFHQLLG
                     SASRDPGDGAFAIELEGLKGSEQTYLPNTAILKTILHGESGSIEVTDFIPRFYTRNRP
                     FRPQTLIRRVRVTEGRPRIRIKMRPRFKYGEVAPSVTSGSNHIRYCGPDMTLRLTTDA
                     PVDYILKETVFNLSDPINFVIGPDETLTNSPLGIAREFQEQTHLYWVNWSHRLALQPE
                     WQDAVIRAAITLKLCSYEPTGAIVAAMTTSIPEAPGTGRNWDYRFCWVRDSYFVIRAL
                     TSLAAGRTLENYYRWIMNIVAATNGHIQPVYGIALEEALVERTADHLPGFQSHGPVSN
                     RPVRIGNQAYEHFQHDTYGNLILGSAQAFIDKRLSVRAGIDDFHKLEAVGEQAFKLHD
                     EPDAGIWELRSRARVHTSSSVMCWAACDRLAKIASYVGEHDRVTFWRQRAELIRDKIL
                     KCSWSDKRKAFVESFGGEHLDASVLLMGELGFVDGQDPRFLSTVTQMEQILGRGPFMM
                     RYEAADDFGVPETAFNVCAFWRLDALARAGKKEQAREIFQSLLNARNHLGLMSEDTDP
                     VSGAAWGNFPQTYSMVGIINGANRLSKPWEAFV"
     gene            2546..>2990
                     /locus_tag="VNR65_06860"
     CDS             2546..>2990
                     /locus_tag="VNR65_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWJ18428.1"
                     /translation="MSRLVIVSNRVADLDNQVQSGGLAVALGDALRSIGGIWFGWDGN
                     IVEEGTPIEVKRTVQNDVTVATIPMTDRDYEEYYLGFANKVLWPVCHYRLDLVKFEQA
                     YLEGYRRVNKGFASALAPLLRPDDLVWAHDYHLITFGAELRALGAR"
BASE COUNT          652 a          827 c          837 g          674 t
ORIGIN      
        1 gctgttttgc ctattttgcg cgatgcaagc gcctacagct tctttttcga ttttgatggc
       61 actctaaccg agatcgccga aacccccggc gcggtcgttg tcgaaaggcg tgctcgcgaa
      121 gcgttggaga tgctttttga aagcgcctca ggtgcagttg ccatcgtgac aggcagagaa
      181 attgactcca ttgacaagtt cctagctccg ctgaaactgc ccgtagctgg cgttcacggt
      241 tacgagcgtc ggaacgggga gaccacggtc tcggcgacag cggcggaaga aagtgcggcg
      301 cgtgcggtcg cgaaagtctt cacagccttc gtcgatcaca accccggtct tcttcttgag
      361 aaaaagcggg gtgcgctcgc cttgcattac cgtctacgtc cggaactcga aactttttgc
      421 ctgtccctgg ccgaagacct ttcctgccgc tttgcggacg tagtcttgac gcgcgggaaa
      481 atggtggttg agatgcggct gcacacggcc acaaaaggca cggctatcaa cgattttctt
      541 caggaaaagc ctttcaggga tcgccttcca ttttttgccg gcgatgacgt aaccgatgaa
      601 gacgcgttcg agacggtcaa tactctcggc ggaattacag tcaaggttgg cgccggtgag
      661 acggctgccc gcgctcgcgc agccagcgtc gatgagtttc tcacctggct cttggcgacg
      721 gctcaatgct cgaaggaatt agggtaggca tggcaaataa tctcgatctt gggcttattg
      781 gaaattgcag catcagcgct ctcgtcgaca aagaggcgcg catcgtttgg tgttgtcttc
      841 caagattcga cggcgatcct gtctttcatc agctcctcgg aagtgcgagc cgcgatcccg
      901 gcgacggcgc gtttgcaatc gagcttgagg gcctcaaagg ctccgagcag acataccttc
      961 ccaatacagc catcctgaaa accattcttc acggcgaaag cggatccatc gaggtcacag
     1021 acttcatccc tcgcttttac acccggaacc ggcctttccg gccacagacc cttatccggc
     1081 gcgtcagggt cacggaggga aggccacgca tccgcatcaa gatgaggcct cgtttcaaat
     1141 atggcgaggt ggctccttcg gtgacctccg gttcaaacca tatccgttac tgcggaccgg
     1201 acatgaccct gcgtctgaca accgatgccc ctgtcgacta cattctcaaa gaaaccgtct
     1261 tcaacctcag cgatccaatc aactttgtca tcgggcccga cgaaacccta accaacagcc
     1321 ctctcggcat tgcacgcgaa ttccaagaac agacgcacct ttattgggtc aattggagcc
     1381 atcggcttgc gctgcaaccg gagtggcaag atgccgtgat cagggcggca atcacgctca
     1441 agctctgctc ttacgagcca acgggcgcta tcgtcgctgc gatgacaaca agcattccgg
     1501 aggctcctgg tacaggcaga aactgggatt accggttttg ctgggtccgc gactcttatt
     1561 ttgtcatccg cgctttgacg agcctcgccg ccggccgcac cctcgagaat tattatcgct
     1621 ggattatgaa cattgtcgcc gccacgaacg gccacatcca gccggtatac ggaattgcgc
     1681 ttgaggaagc gctcgtggaa cgtaccgcgg atcatttgcc cggatttcaa agccatggcc
     1741 cggttagcaa ccgtccagtt cggataggca atcaagccta tgaacacttc cagcacgaca
     1801 catacggcaa tcttattctt ggctcagcac aagctttcat tgacaaaaga ctttcggttc
     1861 gagcgggaat cgacgacttt cacaaattgg aggccgtggg cgagcaggcg ttcaaacttc
     1921 atgatgaacc cgacgcaggc atctgggagc ttagatcgag ggcaagggtt catacatcat
     1981 cgagcgtgat gtgctgggcc gcatgcgatc gcctagccaa gattgcgtcc tatgtcggag
     2041 agcatgatcg ggtgacattc tggcgccaaa gggctgagct gatacgggac aagatcttga
     2101 aatgctcctg gtcagataaa cgcaaagctt tcgtcgaaag ctttggcggc gagcacctcg
     2161 acgccagcgt gttgttgatg ggcgaacttg gcttcgttga cgggcaggat ccgcggttcc
     2221 tttctacagt cacgcaaatg gaacagatcc tggggcgagg gcctttcatg atgcgatacg
     2281 aggccgccga cgactttggt gtcccggaaa ccgctttcaa tgtttgcgct ttctggcgct
     2341 tggacgcttt ggctcgcgct ggcaaaaagg agcaggccag agaaatattt cagtctcttt
     2401 tgaacgctcg caatcattta gggcttatgt ctgaagacac agaccctgtc agtggcgccg
     2461 catggggaaa ctttccccaa acttactcga tggtcggtat aataaatgga gcgaaccggc
     2521 tttcaaaacc atgggaggca ttcgtttgag ccgactcgtc atcgtatcga accgcgtcgc
     2581 ggacctcgat aatcaggttc aatcgggggg gctggcggtc gccctcggag atgcacttcg
     2641 ttcgattggc ggcatttggt ttggatggga cggcaacatt gtcgaggaag gaacgccgat
     2701 cgaggtcaaa aggaccgtcc aaaacgatgt gacagttgcc accatcccca tgacggacag
     2761 ggattatgag gaatattacc ttgggttcgc caacaaggtt ctatggcctg tttgtcatta
     2821 ccgccttgac ctcgtcaagt ttgagcaggc ttacctcgag ggctacaggc gcgttaacaa
     2881 gggctttgcg agtgcacttg cgccccttct gcggcctgac gacctcgtgt gggcccacga
     2941 ttatcatctg ataacgtttg gggcggagct tcgcgccctg ggagcccggc
//
LOCUS       DATMGX010000426         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_105311, whole genome shotgun sequence.
ACCESSION   DATMGX010000426 DATMGX010000000
VERSION     DATMGX010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105311"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="VNR65_06865"
     CDS             <1..1490
                     /locus_tag="VNR65_06865"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWJ18429.1"
                     /translation="KPAFVQACMDAVRDLAKISADGGPALVVGTPWAESGKVYNAVAL
                     LAAGKVETVRYKVDLPNYSVFDEKRVFAAGALPGPVNFNGVRLGIPICEDIWTDDVTE
                     CLAENGAEILLVPNGSPFTLKKRELRENVTVARVAETGLPLLYVNQFGGQDELVFDGA
                     SFALNRGGKLAVQLPAWETALCVTEWRREAGGWTCMPGPLTIIEEGSEAAYLACVLGL
                     RDYVEKNRFPGVVLGLSGGVDSALCAAMAVDAIGAARVHCLMMPYRFTSNASLSDAAA
                     CAEALGVRYDIVPIHSAVEGFDAMLKPMFEGTERGIAEENLQSRIRGTTLMAVSNKFG
                     GMVVTTGNKSEVSAGYATLYGDMNGGFNPIKDLYKTQVFTLSRFRNAARPKGCLGPTG
                     VVMPENIITKAPTAELRENQTDQDSLPPYDALDDILESLVDREEPLSAIVKRGHSPET
                     VKKVERLLYLSEYKRRQAAPGVKITERNFGRDRRYPIVNRFRESL"
     gene            1780..>2565
                     /gene="gltX"
                     /locus_tag="VNR65_06870"
     CDS             1780..>2565
                     /gene="gltX"
                     /locus_tag="VNR65_06870"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWJ18430.1"
                     /translation="MSVKVRFAPSPTGRLHIGNIRPAVFNWLFARREKGTFLLRFDDT
                     DLERSREEYVTGIRDDLTWLGLTWDEEFRQSPRFAFYEETAEKLKQADRLYACYETAE
                     ELERRRKRQMARGHPPVYDRAALKLTKEEKREFEEEGRKPHWRFLLEQRPTAWDDLIR
                     GRQEIDANSLSDPVLIREDGTPLYTFTSVADDTEIGITHIIRGEDHVTNTAVQLQIFE
                     ALGAKAPTFAHHNLLVGAQGEALSKRLGSLSIQGLREQGFEPLA"
BASE COUNT          515 a          761 c          787 g          502 t
ORIGIN      
        1 tgaagccagc ctttgtccag gcttgcatgg acgcggtgcg cgatcttgca aagattagcg
       61 cggacggagg gcccgctctg gtcgtcggca cgccctgggc cgaaagcggc aaagtctaca
      121 atgccgtggc tctgctggcc gctggcaagg tcgaaacggt gcgctacaag gtcgatctgc
      181 cgaattacag cgtcttcgat gagaagcggg tttttgcagc cggagcattg cccgggccgg
      241 tgaacttcaa tggcgtgcgc ctgggcatcc cgatttgcga ggacatctgg acggacgacg
      301 tgaccgaatg ccttgccgaa aacggggcgg aaatccttct cgtgcccaac ggttcgccct
      361 ttacgctcaa aaagcgcgag ctgcgcgaga acgtgaccgt ggcgcgggtt gccgagacgg
      421 gcttgccgct cctctatgtc aatcagttcg gcggccagga cgaactcgtt tttgacggcg
      481 cgtccttcgc gctgaaccgg ggcggaaagc ttgcagtcca gttgccggcc tgggaaacgg
      541 cgctttgtgt caccgaatgg cggcgcgagg ccgggggctg gacatgtatg ccggggccgt
      601 tgacgatcat cgaagagggc tctgaggcgg cctatctcgc ctgcgttctc ggcctgcgcg
      661 actacgtgga gaaaaatcgc tttcccggcg ttgtgctcgg cctttcgggc ggcgtcgatt
      721 cggcgctgtg cgcggcgatg gcggtggacg caatcggcgc agcccgcgtg cattgcctga
      781 tgatgcctta tcggttcaca tcgaatgcat cactgtccga tgcggccgca tgcgccgaag
      841 cgcttggcgt ccgttacgac attgtgccaa tccacagcgc cgtcgaaggg ttcgatgcga
      901 tgctaaagcc gatgtttgag ggaaccgagc gaggcatcgc ggaggagaac ctgcaatcgc
      961 gtattcgcgg cacgacgctg atggccgttt ccaacaagtt cggcggtatg gtcgtcacca
     1021 cgggcaacaa gtctgaggtc tcggcgggct acgccacgct ttacggcgac atgaacggcg
     1081 gctttaaccc tatcaaagac ttgtacaaga cgcaggtctt taccctgtcc cggttccgga
     1141 atgccgcgcg cccgaagggc tgcctcggcc caactggcgt ggtgatgcct gaaaatatta
     1201 tcaccaaggc tcccaccgcc gagcttcgcg aaaatcagac ggaccaggat tcgctgccgc
     1261 cctacgatgc tctcgacgac atcctggaaa gcctcgttga ccgggaggag ccgctttcgg
     1321 cgatcgtgaa gcgcggacat tcgcccgaga ccgtcaaaaa agtggagcga cttttgtatc
     1381 tgtccgagta taagcggcgg caggcagccc cgggtgtcaa gatcactgaa cgaaatttcg
     1441 gccgggaccg ccgctacccc attgtcaatc gcttccgcga gtcgctctga gaatgaaccc
     1501 taaagcgtgt cgaaattatt cggaatcccc ttattgtcat ggccgccctc cgaggcggcc
     1561 atccaggatc gcaaattcca tctttttgga gcttgtggct ggatggccgg gtcaagcccg
     1621 gccatgacat cggttccagc taatcgcgag acgttttaac gtcatcgcgg aaaggagcga
     1681 acgccttatc cgcgatctta gaagatgctt gagtattgaa agatcccaga taatcgccac
     1741 ggcgctttcc gtgatgacaa tcaggttttg gaggacgtaa tgtccgtaaa ggttcgcttt
     1801 gcgccgagcc ccaccggccg gcttcacatc ggtaatatca ggccagccgt tttcaattgg
     1861 cttttcgccc gccgcgagaa gggcaccttc ctcttacgtt tcgatgacac ggacctcgag
     1921 cgctcaagag aggagtacgt cacgggcatc cgggacgacc tgacctggct cggccttacc
     1981 tgggacgagg agttccgcca atccccacgc ttcgctttct atgaggagac ggccgaaaag
     2041 ctgaagcagg cggacaggct ctacgcttgc tacgagacag cggaagaact ggagcgacgc
     2101 cgcaagcggc agatggcgcg cggtcaccca cccgtctacg accgtgcggc gctcaagctg
     2161 accaaagagg aaaaacgcga gttcgaagaa gagggccgca agccgcactg gcgtttcctc
     2221 ctggaacaac ggccgactgc ctgggacgat ttgatccgcg gccggcagga gattgatgcg
     2281 aattccctct ccgatcccgt tttgatccgc gaagacggca cgccgctcta cactttcacg
     2341 agcgtcgccg acgacaccga aatcggcatc acacacatca ttcgcggcga agatcacgtc
     2401 acgaacactg ccgttcaact ccagattttc gaggcgctgg gcgcgaaggc gccaacattc
     2461 gcgcatcaca atcttctggt cggggcgcag ggcgaggcgc tgtctaagcg gctcggttcg
     2521 ctctcgatcc agggtcttcg cgagcaaggc ttcgagccgc tggcc
//
LOCUS       DATMGX010000427         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_448002, whole genome shotgun sequence.
ACCESSION   DATMGX010000427 DATMGX010000000
VERSION     DATMGX010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448002"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(497..>2136)
                     /locus_tag="VNR65_06875"
     CDS             complement(497..>2136)
                     /locus_tag="VNR65_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HWJ18431.1"
                     /translation="PAQISEMDQLVARKDIAWPTREDFIERNFECYTGLNIDPISVCF
                     IDGSILNNRPFREAISAIGNHPAYREVDRRIVYIDPDPAAAGTAFRRRSPGVFLSLKS
                     ALLDLPRTQPVTDEFKWIRDYNERALKFREVVGNVRPSIRARVAQSVTRPLDQMLTAD
                     DIRRAREEIADTASRDTGFAYEGYVALKLSGVRASVSKMIVALRGSAQQSPFLAVVSA
                     IVAAWALESGATYQEYRPQGDNSSDGSKVPAIPYWIRFLQAFDADYQQRRLRFMVEGL
                     NRLYQDLDRSQHLNIDHAAINGLKRGYYDCLGRLGHGLNISSVSAASCDLADRLFGMV
                     PAPGDMEDLERYAREFVRSHREDIDRLIESLGTDLDLTAVAGKLDNLLASTNFRQLPA
                     SAHTDFLVDYVGFPFWDVLTFPISIWSTTREVNEIKIDRISPQDARTLTEFRGPQALK
                     GVALGHFAAFLSRGYRENDYRLGRLHGLDRLVDIVLSSANIDANSQKEAILLIKQKGF
                     RQILNAEEKHLPNSAALIATIRRAVDELNTNSDIGSS"
BASE COUNT          471 a          620 c          581 g          464 t
ORIGIN      
        1 ggccgataga cagcgaagat gtttatctcg tggggggcta ttgcgagggg tctcactttt
       61 ttctctcttt tgcgcgacac attcgttgaa gcgcggagtt ggctggtgaa ttagctcgtg
      121 ggtgggatcc cgatcagcgc cgatcgagat ccgcgctttg cgctgatttt ccactaccta
      181 tcaaggtagt atggcgaact ctggcggggt tccgcttcgg tactgattca caccgtcgag
      241 gcaacgtgaa agcgcatcgg cgctttgatt ggatgcttca cataacagga atgcgccaac
      301 tacttcgcaa acaccggata cgctttcgca aacgccggat acgcttcaga ataaaacaat
      361 catgctctag tggacattcg aggcttcggt cattagcgcg gaatcccgcc cccgcgtgac
      421 caggcacaca gacctatagg cccacgatag cggatcctga ggcatggttt caaagcggcc
      481 tcaagccgca cgaggcctac gaacttccga tatcggagtt cgtattcaac tcgtccacag
      541 cgcgacggat cgtggcaatt agcgcggcgc tattgggcag gtgtttctct tcggcattca
      601 aaatctgacg aaatcctttt tgctttatca aaagaatcgc ctccttctga ctgttggcgt
      661 caatattcgc cgagctgagg acgatgtcga ccaatcgatc caacccatga agccttccca
      721 aacgatagtc attttcacga taaccgcgtg aaagaaacgc cgcgaaatgt cccagagcaa
      781 cgcccttgag cgcctggggg cctctgaatt ccgtcagcgt cctggcatcc tgcggactga
      841 tgcgatcgat cttgatctca ttcacctcgc gcgttgtgct ccatatgctg atcgggaacg
      901 tcaggacatc ccaaaatgga aagccgacat aatcaaccag gaaatcggtg tgcgccgagg
      961 cgggcagctg gcggaagttc gtcgatgcaa gcaagttatc gagcttgccg gcgaccgcag
     1021 tgagatccag gtcggtgcca aggctctcta tcagccggtc gatatcctcg cggtgactcc
     1081 tgacgaactc gcgcgcatag cgttcgagat cctccatatc gcccggcgcc ggcaccatgc
     1141 cgaagagccg atcggcgaga tcgcagctgg cggcggaaac ggacgaaatg ttaagtccat
     1201 ggccgagcct gcccaggcaa tcataatagc cccgcttcaa tccattgatt gcggcatggt
     1261 cgatgttcaa atgctgcgat cgatccagat cctgataaag ccggttcaga ccttcgacca
     1321 tgaatctcaa ccgccgctgc tgataatcgg cgtcgaacgc ctgcaggaac ctgatccagt
     1381 agggtatcgc cggaaccttt gacccatcgg aactattatc cccctgcggc cgatattcct
     1441 gatatgtggc gccggattcg agtgcccagg cagcgacgat ggcggacacg acggccaaga
     1501 acggcgactg ttgtgcgctg ccgcgcaagg ctacgatcat cttgctgacc gatgcgcgaa
     1561 cgccggagag cttgagcgcc acatagcctt cataggcgaa tcccgtgtcg cgtgaggccg
     1621 tgtcggcaat ttcttcacgg gcccttcgta tatcgtccgc cgtcagcatt tgatcgagcg
     1681 gtcgcgtcac cgattgcgcc acgcgggccc tgatactcgg cctcacattc ccgacgactt
     1741 cgcgaaactt cagcgccctc tcattgtaat cgcgaatcca tttgaattca tcggtcacgg
     1801 gctgggtccg aggcagatcc agcagtgcgc tcttcaacga cagaaaaacg ccagggctgc
     1861 ggcgacgaaa tgcggtacct gccgcggccg gatccggatc tatatagaca atacgccgat
     1921 ccacttcgcg ataggccgga tgattgccga tcgccgagat cgcttcccgg aacggccggt
     1981 tgttgagtat gcttccgtca atgaaacata ccgatatcgg atcgatattc agaccggtat
     2041 agcactcgaa attccgctcg atgaaatctt cgcgcgtcgg ccatgcgata tcttttcgcg
     2101 caacaagctg atccatctcg gagatttgcg ccgggc
//
LOCUS       DATMGX010000428         2572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_225531, whole genome shotgun sequence.
ACCESSION   DATMGX010000428 DATMGX010000000
VERSION     DATMGX010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225531"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VNR65_06880"
     CDS             complement(<1..1156)
                     /locus_tag="VNR65_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWJ18432.1"
                     /translation="MNSMDKNLNIPPSRQREKPGQNLKWIGQNMKRVEDPRLLTGQGR
                     YIDDIDLPNLLHAAVLRSSRAHARIKSISIEAAKKLPGVFKVLTGEDVAKATGPLPCF
                     ANPPVEQRCIAVGKVRHVGEPVAVVVAENRYIAEDAVDLIEVEYEELPVVVDALAAMT
                     SSGDAVLHPERGQNNIAHQKRFKFGSVDEDFAKAAKVIRRHLLWPRSGAQPMETVGAI
                     AQYDSGTGKFNVHINGSMYNYLGFTMAGALGIPGHKLNLIPCIAGGSFGSKLFTHKVP
                     ILAASLARICRRPVKYIEDRIDNTTACDNHGSDRIYDAELALDADNNMIGLRCKVIDD
                     YGAYFQYGVGHHGNALSQITGPYQIRSVDLDLTAVFTNKCQQGAYRGFGSE"
     gene            complement(1153..1653)
                     /locus_tag="VNR65_06885"
     CDS             complement(1153..1653)
                     /locus_tag="VNR65_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWJ18433.1"
                     /translation="MARQAVSVTVNGTVYERQVEPRLLLSDFLRHEINLPGTHVGCEH
                     GVCGVCTVLIEGKAARSCLCFAIQADGLSVETVESLGSVEELHPLQQGFWEKHGLQCG
                     FCTPGMLMSAVELLRNNPTPDHEAIREAIGGNLCRCTGYQNIIASIERAVEITLARAQ
                     QPEAAQ"
     gene            complement(1746..>2572)
                     /locus_tag="VNR65_06890"
     CDS             complement(1746..>2572)
                     /locus_tag="VNR65_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWJ18434.1"
                     /translation="EAIAALGRVDQDAKVLAGGQSLIPMMNLRMVKAERLIDIGRIKS
                     LGSIKEVGGELRIGSMVTHNTILSSPVVAKVCPLLVEAYHEVAHHSVRNRGTLGGSLC
                     HNDPGAEMPLIVSILGATLVARSTNGERLIAADDFFQGSFATALEPNELLVEIRIPVP
                     PAGHGYAFLEVNQRKGDFALVACAAMLTIESGACRNARIGYRSIGSNTARFPSIEALL
                     EGHRPTPELFAQVAATASETANPTADVHADIAFRRDLIRTLTQRVLATAAERASRR"
BASE COUNT          518 a          778 c          719 g          557 t
ORIGIN      
        1 cttctgagcc gaagccccgg tacgcgccct gctggcattt attggtaaac accgcggtca
       61 aatccagatc gaccgaacgg atttgatagg gccccgtgat ctgcgagaga gcgttgccgt
      121 gatgaccaac tccgtactgg aagtaagcgc cgtaatcgtc gatgacctta cagcgcagcc
      181 cgatcatatt gttatcggcg tcgagggcga gttctgcatc gtagatgcga tcagacccgt
      241 ggttgtcaca ggccgtcgtg ttatcgattc gatcctctat atatttcacc ggccgcctgc
      301 agattcgggc cagcgatgcc gcaagaattg gcaccttgtg cgtgaacagc ttgctgccga
      361 agcttccgcc ggcgatgcag ggtatcaggt tgagcttgtg tccgggtatg ccgagcgcac
      421 ccgccatggt aaagccgaga tagttgtaca tcgacccatt gatatggacg ttgaattttc
      481 ccgttccgct atcgtactgg gcaatcgcgc cgaccgtctc catcggttgg gcaccagaac
      541 gaggccacaa gaggtggcgg cgaatgacct tggcggcttt cgcgaaatct tcatccacgg
      601 acccgaactt gaaccgcttc tgatgggcaa tgttgttctg cccccgttcg ggatgcaaaa
      661 ccgcatcgcc tgacgacgtc atggcggcaa gcgcatcgac gacgaccggt agttcctcat
      721 attcgacctc aatgagatcg accgcgtcct cggctatata gcggttttca gccaccacaa
      781 ccgccaccgg ctcgccgaca tgacgcacct tgcctaccgc gatgcaacgc tgctcgaccg
      841 gcggatttgc gaaacaaggc aacggccccg tcgctttggc gacgtcttcc cccgttagaa
      901 ccttgaagac gcctggaagc tttttggcgg cttcgatgga gatcgactta atgcgcgcgt
      961 gcgcgcgtga actcctaaga accgcggcgt gcagcaggtt ggggagatcg atgtcatcaa
     1021 tatagcgtcc ctgaccggtc aataggcgcg gatcttcgac ccgcttcatg ttttgaccga
     1081 tccactttag attttggccc ggcttctcgc gctggcggct cggcggaatg ttgagatttt
     1141 tgtccatcga attcattgtg cagcctccgg ctgttgagcg cgggccaggg tgatttcaac
     1201 cgcgcgttcg atcgatgcga taatgttctg gtagcccgtg caacggcaaa gatttccccc
     1261 gatcgcctct cgaatggctt cgtggtccgg ggtagggttg ttgcgcagca attcaacagc
     1321 actcatcaac atccctggcg tacagaaccc gcattgcagg ccgtgttttt cccagaagcc
     1381 ctgctgcaag gggtgaagct cctcgacgct gccgagagac tcgacggttt cgacggaaag
     1441 gccgtccgcc tgaatagcaa agcacaggca ggaccgtgcc gcttttccct cgatcaggac
     1501 tgtgcagacg ccacacacgc cgtgttcgca accgacatgc gttccaggca ggttgatttc
     1561 gtgtcggagg aaatcactca gcagcagcct cggttcaacc tgccgttcgt agaccgtgcc
     1621 attgaccgtc acggaaaccg cttgtcttgc catttgaatc ctccctcgat ttattctcaa
     1681 ttgctggcca gcgctttcca gtcgtcggcc cgatctccaa tccaactaga aagcgcggat
     1741 gtggcctacc gccgcgaggc tcgctcggct gcggtggcca aaacgcgctg cgtaagcgtt
     1801 cggatcagat ctcgcctgaa cgcgatgtct gcatgaacgt cggctgtcgg attcgccgtt
     1861 tcgcttgcag tagccgcaac ctgcgcgaaa agctcaggcg tcggccggtg gccttcaagc
     1921 agcgcttcga tgctcggaaa tcgcgcggta ttggagccga tgctccggta tccgatccgc
     1981 gcattgcgac atgcaccact ctcgatcgtc agcattgccg cacaggcaac gagcgcgaag
     2041 tccccctttc tttggttcac ttcgagaaag gcatacccat gccccgcagg cggaaccggt
     2101 atccgaatct ccacaagaag ctcattcggc tcgagcgctg tcgcgaaact accctgaaag
     2161 aaatcgtcgg cggcgatcag ccgctcacca ttcgtacttc gcgcgacaag agtggcgccc
     2221 agtatcgaga ctatcaaggg catctctgca ccggggtcat tgtggcaaag gctgccgccg
     2281 agcgttccac ggttccgcac cgagtgatgc gccacctcat gataggcttc gaccaacagc
     2341 gggcagactt ttgcaacaac cggcgaagac aagatggtgt tgtgtgtgac catcgacccg
     2401 atgcgaagct caccgccgac ctccttgatg gatcccaagc tcttgatgcg gccgatatcg
     2461 ataagccgct ctgcttttac catgcggaga ttcatcatag ggatcaaact ctgcccgccg
     2521 gcgagcacct tggcatcctg gtcaacgcgg ccgagggcgg caatggcttc ct
//
LOCUS       DATMGX010000429         3866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_364852, whole genome shotgun sequence.
ACCESSION   DATMGX010000429 DATMGX010000000
VERSION     DATMGX010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364852"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..573
                     /gene="rplP"
                     /locus_tag="VNR65_06895"
     CDS             157..573
                     /gene="rplP"
                     /locus_tag="VNR65_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWJ18435.1"
                     /translation="MLMPKKVKYRKQQRGRMCGKAWRGGELSFGDFGLKVMEPGWITD
                     RQIEASRVAMTRFVKRGGKIWIRLFPDKPVTKKPAETRMGKGKGAPDHWVAVVRPGKI
                     LFEMEGVSVADAAEAMRLASHKLPLRTKFVQREGVH"
     gene            708..995
                     /gene="rpmC"
                     /locus_tag="VNR65_06900"
     CDS             708..995
                     /gene="rpmC"
                     /locus_tag="VNR65_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWJ18436.1"
                     /translation="MNVDKIRNLTDIELAHQQRDMADQLFKMKFQLKMGQTESLKKIR
                     ELKKDIARVQTVLRQKELAGGNGAAASASNPVKKTVTAKKAASKKAATEKK"
     gene            999..1319
                     /gene="rpsQ"
                     /locus_tag="VNR65_06905"
     CDS             999..1319
                     /gene="rpsQ"
                     /locus_tag="VNR65_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWJ18437.1"
                     /translation="MAETTAATSTAKTPGRRKTLIGEVVSTKMAKTIVVEVRRQKAHP
                     FYKRVVARSKKFYAHDEDGTAHLGDFVKLEETRPLSKLKRWQLKEIVRHAAQIAPVEA
                     KPAS"
     gene            1368..1739
                     /gene="rplN"
                     /locus_tag="VNR65_06910"
     CDS             1368..1739
                     /gene="rplN"
                     /locus_tag="VNR65_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWJ18438.1"
                     /translation="MAVMMRSMLDVADNSGARKLQMILPLGGSTGLVAHLGDVVTASV
                     KEASPDGTAKKGTVVKAVIVRTRKEHRRRDGTYIRFDQNAAVLINDANEPVGTRVFGP
                     VARELREKKFLKIVSLAPEVL"
     gene            1863..2189
                     /gene="rplX"
                     /locus_tag="VNR65_06915"
     CDS             1863..2189
                     /gene="rplX"
                     /locus_tag="VNR65_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWJ18439.1"
                     /translation="MQKSVDIRRNDQVRVITGRDKGKEGRVLRVMPDEFKVLVEHVMM
                     VKKNVRPNPQKNIKGGIAEQESPISISNVMLMCSSCARPVRVGHEKHGDRQSRVCKNC
                     STTLEK"
     gene            2565..3095
                     /gene="rplE"
                     /locus_tag="VNR65_06920"
     CDS             2565..3095
                     /gene="rplE"
                     /locus_tag="VNR65_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWJ18440.1"
                     /translation="MRDRYYKDVLPALMKEFDLDNPMAAPKLEKIIVNMGVGEATQNA
                     KLIDPAAGEMGQITGQKPVITRARKSIAAFKVREGMPIGVMVTLRGDRMYEFFDRLVN
                     VALPRVRDFRGVPTKSFDGRGNFTLGVRDQLIFPEIDYSKVEKMKGMNITIVTSAGTD
                     DQARALLKHMGMPFRQ"
     gene            3197..3382
                     /locus_tag="VNR65_06925"
     CDS             3197..3382
                     /locus_tag="VNR65_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HWJ18441.1"
                     /translation="MAKTSSIAKANKKPKFPVRQHNRCHICGRPRAFLRKFGICRLCF
                     RGLALRGEIPGVMKSSW"
     gene            3502..>3866
                     /gene="rpsH"
                     /locus_tag="VNR65_06930"
     CDS             3502..>3866
                     /gene="rpsH"
                     /locus_tag="VNR65_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWJ18442.1"
                     /translation="MSLTDPVSDFLTRVRNAIKARHQKVDVPHSKLKVEMARILKEEG
                     YIANFKAAEEEGRKVLRVYLKYSPDNSSPISDLKRVSRPGCRVYVGHTEIPRVLGGLG
                     ISILTTPRGVMTGRQARKEG"
BASE COUNT          947 a          975 c         1242 g          702 t
ORIGIN      
        1 gtagctgggc agagtgggag ttaggacaga aggtttctaa tccctagcca gtaaccccag
       61 tccatgaacg tgggagatgc tgggcggtag tcggaaaggt gagttagaac aggtttttct
      121 aaccccttag tccctaaccc ctagccattg aaacttatgt tgatgccaaa gaaagtgaag
      181 tatcgcaagc agcagcgcgg ccgcatgtgc ggcaaggcat ggcgcggcgg cgagctgtcg
      241 ttcggcgact tcggcttgaa agtcatggag ccgggttgga tcaccgatcg gcagatcgag
      301 gccagccgtg tggccatgac ccgctttgtc aagcgcggcg ggaagatctg gatccggttg
      361 tttccggaca agccagtcac caagaagccg gccgaaaccc gtatgggcaa aggcaagggg
      421 gcgccggacc actgggtagc tgtagtgcgt cccggtaaga tcctcttcga aatggaaggg
      481 gtgtcggtgg ccgatgccgc cgaggcgatg cgtctggctt cgcacaagct gccgcttcgc
      541 accaagttcg tgcagcgcga gggcgtgcac tagaaagcag ttgccagtgg tcagtagcca
      601 gtagaaggcg gccacatgga aaggtattga cgaattaaga agtagtgccg gcggctggca
      661 gtgaaaactg gcaactggct accggcaact gacaactggg ttcaactatg aatgtagaca
      721 agattcgcaa tttgaccgat atcgaactgg cccatcaaca gcgggacatg gctgaccagc
      781 tcttcaagat gaagttccag ttgaagatgg gacagaccga gagcctgaag aaaatccgcg
      841 agctgaagaa agacatcgct cgtgtgcaga cggtgttgcg ccagaaagag ctggcgggcg
      901 gtaacggcgc agcggcaagc gcaagcaacc cggtgaagaa gacagtgacg gcgaagaagg
      961 cggccagcaa gaaggctgcg acggagaaaa agtaagccat ggccgaaaca acagcagcaa
     1021 ccagcacagc aaagaccccc ggccgtcgca agaccctgat tggcgaagtg gtctccacca
     1081 agatggccaa gaccatcgtg gtcgaagtcc ggcggcaaaa ggcccacccg ttctataagc
     1141 gcgtggtggc ccgcagcaaa aagttctacg cccacgacga ggacggcacg gcgcatctcg
     1201 gcgacttcgt gaagctggaa gagacccggc cgctctccaa gttgaagcgg tggcagttga
     1261 aggaaatcgt gcggcacgcg gcgcagatag cgcccgtcga ggcaaagccg gcgagttaag
     1321 ccggtggtgt tggctccgcc catgcggtgg agcaaggaga caagattatg gcagtgatga
     1381 tgagaagcat gctcgacgtc gccgacaact ccggtgcgcg caagctgcag atgattctgc
     1441 cgctgggcgg ttccaccgga ttggtcgccc atttgggtga cgtggttacg gcttccgtga
     1501 aggaggcgtc tcccgacggc accgccaaga agggcacggt ggtgaaagcc gtgatcgtgc
     1561 gtacgcgcaa agagcatcgc cgccgtgacg gtacctacat ccgcttcgat cagaatgcgg
     1621 cggttttgat caacgatgcc aacgagccag tcggcacgcg cgtgttcggc ccggtcgccc
     1681 gtgaactacg cgagaagaag tttttgaaga ttgtttccct ggcgccggaa gtgctgtaag
     1741 agcaactagc aactagtaac tagcaactag taactagcga tgagtaagaa gtaaattttg
     1801 cgaaagtttt gagaagagct agttgctaat tgctagctgc tagttgctaa ggggtttgag
     1861 cgatgcagaa gagtgtagat atccgccgca acgatcaggt gcgggtgatt accggacgcg
     1921 acaaaggcaa ggaaggccgc gtgctccggg tcatgccgga cgagttcaag gtgctggtcg
     1981 agcacgtcat gatggtcaaa aagaacgtgc ggcctaaccc gcagaagaac atcaagggcg
     2041 gcattgccga acaggagagc ccgatttcca tcagcaatgt aatgctgatg tgcagctcct
     2101 gcgcgcgtcc cgtgcgggtg ggccatgaga aacacggcga ccgccagtct cgcgtatgta
     2161 agaactgcag caccacgttg gagaagtagc tggtggcagt tggcaattag catttggcag
     2221 ttagctaatg cacaactctt gagtgttctt gtaagtggaa cgcggaattt tttggggcaa
     2281 cgagcagaaa tttgaggcat tggctaaatg ctaattgcca agtgctaaat gcccaattga
     2341 actcctcacg aaccggtagc agcaacaaac cgaaaccgtg agaaaggtag aagcagggaa
     2401 tggcagacga gaagaaaccg aaagcgcagc agcagggtgg cgagggaaaa gcgcagggca
     2461 agccccaggg aaagaaggcg aaacccgcga aggacgaagt gacggcggag gcggcttcgg
     2521 gcaaaccggc gcggcccaag gcgaccaatc gccagaccgc tcgcatgcgc gaccgctatt
     2581 acaaggatgt tttgccggcg ctcatgaaag agttcgacct cgacaaccct atggcggctc
     2641 cgaagctgga aaagatcatt gtgaacatgg gagtgggcga ggcgacgcaa aacgccaagc
     2701 tgatcgatcc ggcggcgggc gagatgggcc agatcaccgg tcagaagccg gtcattacgc
     2761 gggcgcgtaa gtccatagca gccttcaagg tgcgcgaagg aatgcccatc ggcgtgatgg
     2821 tcacgctgcg cggtgaccgc atgtacgaat ttttcgaccg cctggtgaac gtggcgctgc
     2881 cgcgtgttcg cgacttccgc ggtgtgccga ccaagtcctt cgatggacgc ggcaatttca
     2941 ccctgggcgt gcgcgaccag ttgatttttc cggagatcga ttactccaag gtcgagaaga
     3001 tgaagggcat gaacattacg atcgtgacct cggctggcac tgacgaccag gcgcgtgcct
     3061 tgttgaaaca catgggcatg ccgtttaggc agtagccagc agtcagtagt cagcgagtgg
     3121 tttcaggttt tgagtttcaa gctaacagct aaaggctaag agctaatagc tagaggcgaa
     3181 tagcgaaaag gaaaagatgg cgaagacatc cagtattgcg aaggcgaaca agaagcccaa
     3241 attcccggtc cggcagcaca atcgctgcca tatctgcgga cgtccgcgcg cctttctgcg
     3301 caagttcggc atttgccgtc tgtgcttccg cggtctggcc ctgcggggcg agattcccgg
     3361 ggtcatgaag tcgagttggt agagcagttg ctagtggcca ggtagtccgt cgcggctggt
     3421 tctttcccga gcgttgatga agcgctggcg cgaagagttc gtgcgaaagc taacgagcga
     3481 cgatttgagg agatatacag catgagtttg accgatccgg tctcagattt tttgacccgc
     3541 gttcgcaatg ccatcaaggc acggcaccag aaggtggacg ttccgcattc caagcttaag
     3601 gtcgagatgg cccgcatcct gaaggaagag ggctacatcg cgaacttcaa agcggccgaa
     3661 gaagaaggcc gcaaggtttt gcgggtgtac ttgaagtaca gccccgacaa ctcgtcgccg
     3721 atttcggact tgaagcgggt ttcgcgtccg ggttgtcgcg tgtatgtcgg acacaccgag
     3781 attccgcggg ttctcggcgg tctggggatc agcatcttga ccactccccg aggcgtaatg
     3841 accgggcgcc aggcgcgcaa ggaagg
//
LOCUS       DATMGX010000430         3867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_168179, whole genome shotgun sequence.
ACCESSION   DATMGX010000430 DATMGX010000000
VERSION     DATMGX010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168179"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VNR65_06935"
     CDS             <1..1025
                     /locus_tag="VNR65_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HWJ18443.1"
                     /translation="LNPRARTLPEPFRAETVNGEANSLYFQFRNPQFLTEGLNETIID
                     ILQRPRFEALLREPVLLTSPPRNGFSNELESTLHDAIHSAVGLREGMGSPEFAARDPI
                     FWIHHANIDRLWESWRLPERDGTSSRDPAANNPWRAIRFRFVDSGGQEATADIALSLL
                     AATNLGYRYDRLEPVQAPPSAFLPASALKAPTMLLESAGAQKAIRTPQDIVTVPLVPS
                     QMQSAIADVTRNPAVRYELSIDLNSGSEPGIYQVFLTSREKEGAMSAEKKIGAFSLFS
                     ARGSQIHQHKASDAKVDTTIRIDITDQVKNNVVNPNKAAEIVIRPSYLAETVSVLVKN
                     VKIIAK"
     gene            1107..1433
                     /locus_tag="VNR65_06940"
     CDS             1107..1433
                     /locus_tag="VNR65_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18444.1"
                     /translation="MFKLIPAAAIAIGVLMGTAQAMPASPSSMPVAGANASAIQSVEY
                     KSYGYHRYGGYGNSGYRSHGYSSYGYNKYRPSYGGYKQYGNNGGYGGGYGGGGYGSGY
                     GGYGKY"
     gene            1571..2284
                     /locus_tag="VNR65_06945"
     CDS             1571..2284
                     /locus_tag="VNR65_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWJ18445.1"
                     /translation="MKTYFILLMLVGLCCPRASPAQQRITPEDMINALKGSGAGPGMS
                     LKKDRDAGANPDRSASKERGGGPLPGNSLTKERIISLLGGDAPQSGDGVYRGLVAKLQ
                     KKEKDRSFTIKDREEVEIFSTDRPKISIEIYFDYNSADITSAAKSMLDTLGSALRDPQ
                     LAGTTFIFRGHTDARGDANYNLQLSDRRAVAVKNYIVRSFKVDESTIASIGDGSRHLK
                     NEANPFADENRRVEVVRSN"
     gene            2447..3541
                     /locus_tag="VNR65_06950"
     CDS             2447..3541
                     /locus_tag="VNR65_06950"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWJ18446.1"
                     /translation="MFESRKRHHSTRTFSGLVADAYAMNEIPTPANSAPRSKRVLVLG
                     ATGTIGRATVSELMRRGNEVVCFVRPRAGVGGALMPTSSAHVLKGATLRFGDVSDPAS
                     LERDGFQGERFDALVSCLASRTGAPKDAWAIDYQAHVAALAAAKKAGVSQMVLLSAIC
                     VQKPLLAFQHAKLAFENVLVESGLTYSIVRPTAFFKSLSGQIDRLQRGKPFLMFGDGE
                     MTACKPISDNDLAQFLAECLDDESRYNRVLPIGGPGEAITPKQQGEHLFALLGREPKF
                     KRVPVALLDTIIWLLGGLGRVIPRLAEKAELARIGRYYATESMLVLDPATGRYDATKT
                     PSAGSETLFDFYARLIKGEASCELGDHAVF"
BASE COUNT          879 a         1089 c         1084 g          815 t
ORIGIN      
        1 acctcaatcc gagagcgcgc acattgcccg agccattccg ggcggagact gtcaacggcg
       61 aagcaaattc gctctacttc cagttcagaa atccccagtt cctgaccgag ggtctgaacg
      121 aaaccatcat tgatatcttg caaaggcccc ggtttgaggc gctccttcgt gaaccggtac
      181 tcctgacgag ccctccacgg aacggcttca gcaacgaact cgaatccacg ctgcacgatg
      241 ccatccatag tgcggtgggc ctgagggaag gaatggggtc acccgagttc gcggcaaggg
      301 atcccatttt ctggattcat cacgcaaata ttgaccgtct ctgggaaagt tggcgtctgc
      361 ccgagagaga cggcacctcc agccgtgatc cagccgccaa caatccatgg agagcgatta
      421 gatttcgttt cgtcgactcg ggtggtcagg aagccacggc agacattgcc ctttccttgc
      481 ttgcggctac caaccttggc taccgctacg atcgtctgga gccggttcaa gcgcctccat
      541 ctgccttcct gccggcgtcc gccctcaaag cgccaaccat gcttttggaa agcgcgggcg
      601 cccagaaagc catcaggaca ccgcaggaca ttgtcacggt tcccctcgtt ccgtctcaga
      661 tgcaaagcgc catcgcggat gtcacgcgta acccagcggt gcgttacgag ctttcaatcg
      721 atcttaattc cggttccgag ccgggcatct accaggtttt cctgacaagc cgggagaagg
      781 aaggcgccat gtccgccgaa aagaaaatcg gggcgttcag cttgttcagt gcaaggggca
      841 gtcaaatcca ccagcacaag gcttccgatg ccaaggtgga cacgacaatc aggatcgata
      901 tcaccgatca agttaagaac aatgtcgtca atccgaacaa agccgccgag attgttatcc
      961 ggccctctta tctggccgag actgttagtg ttttagttaa gaacgtaaag ataatagcga
     1021 agtaaacttc agtgaatttt agaggaattt tttatcacct aaatattgcg gaaagtcaaa
     1081 gtcaagcatc aaacaaggag tacgaaatgt tcaagttaat cccagctgcg gccatagcga
     1141 ttggagtcct tatggggaca gcgcaagcaa tgccagcttc accgtcttcg atgccggtgg
     1201 ctggtgcaaa tgccagcgcg attcagagtg tcgaatataa aagctatggt tatcaccgct
     1261 acggaggcta cggaaattcc gggtaccgct cgcacggata ttcgagctac ggttacaaca
     1321 agtaccgccc ctcctacggt ggctataagc aatacggaaa taacggcggc tacggcggcg
     1381 ggtatggcgg cggcgggtac ggcagcgggt atggcggtta cggcaagtac tgagcccaga
     1441 aggccaggag cgaggggctt ttctacccct gctcctggcg cggatagccc cctgtcctga
     1501 tgagccggtc ggtgtgctgc cgatgctttg aatgatcgcg aggcgacaag ctgcgtgcaa
     1561 agagttcgcc atgaaaactt acttcattct tcttatgctt gtaggacttt gctgcccccg
     1621 cgcctcaccc gcgcaacagc gcatcacgcc ggaggatatg atcaacgcct tgaaaggaag
     1681 cggagcgggg ccgggtatgt ctctgaaaaa agacagagat gccggagcca acccggacag
     1741 gtccgcgtcc aaggaacgcg gaggcggacc cttgcccggc aattctctaa ccaaagagcg
     1801 cattatttcg cttttgggag gcgatgctcc ccaatcagga gacggtgtgt accgcggctt
     1861 ggtcgccaag ctccagaaga aggagaagga tcggtctttc acaataaaag accgagaaga
     1921 agtcgaaata ttctctacag accggcccaa gattagcatc gaaatctatt tcgactacaa
     1981 ttcggctgat atcacaagcg cggcaaagtc gatgctcgac actctcgggt cggcgctgcg
     2041 agatccgcag ctcgcaggta cgacattcat ctttcgcggg cacaccgacg cccgcggcga
     2101 cgcgaactat aatcttcaac tttcggaccg cagggccgtt gcggtcaaaa actatattgt
     2161 gcgctcattc aaggtggatg aaagcaccat cgcatcgatc ggcgatggct cgcggcattt
     2221 gaagaacgaa gccaaccctt tcgcggatga gaaccggaga gtcgaggtcg tgcgctcgaa
     2281 ttgatccgcc accgggttaa ggattttttg ggggcttggt atggattcag cggacgggcg
     2341 ctgagagtcc gtctcatctc gttcagaaga gcaaatcgtc gtaatttctt gcactttaga
     2401 aaacttctgg acaaaacaat tttatgattc gtaatgatgg ggtcgcgtgt tcgagtcacg
     2461 caagcggcac cactcgacca ggactttctc gggcctcgtc gccgatgcat atgccatgaa
     2521 tgaaataccg acaccggcca acagtgcccc ccgctcaaag cgcgtgctgg tattaggcgc
     2581 gaccggcacc attggccgcg cgacggttag cgagttgatg cgccgcggca acgaggttgt
     2641 ttgcttcgtg cggccccgag caggggtggg cggcgcgctt atgccaacca gcagcgcgca
     2701 tgtgctcaag ggggctaccc tgcggttcgg ggacgtttcc gaccccgcat cccttgagcg
     2761 tgacggcttt caaggggagc gctttgacgc tttggtttcc tgcctggcct ctcgaaccgg
     2821 agcgcctaag gatgcgtggg ccattgatta tcaggctcat gtggccgcgt tggccgctgc
     2881 aaagaaagcg ggcgtctcgc agatggtgct gctgtcggca atctgcgttc agaaaccact
     2941 tttggccttt cagcatgcca agctggcgtt cgagaacgtc ctcgtcgagt ctgggctaac
     3001 ctattccatt gtcaggccca ccgccttctt caagtctctg tcgggacaga tcgaccgcct
     3061 tcagcgcgga aagccctttc tgatgtttgg cgacggagag atgacggctt gcaagcccat
     3121 cagcgacaat gatctcgccc aattcctcgc tgaatgcctg gacgacgaaa gccgctataa
     3181 tcgcgtgctc cccatcggtg gcccgggaga agccatcaca cccaaacagc agggcgagca
     3241 tctgtttgcc ttgcttgggc gcgaaccgaa gttcaagcgc gtgcccgtgg cgctgcttga
     3301 cacaataatt tggttgctgg gggggcttgg ccgcgtaatt ccccgtttgg cggagaaagc
     3361 cgagcttgct cgcatcgggc gttattacgc tacggagtcc atgctcgttc tggaccctgc
     3421 aacaggacgg tacgacgcca ccaaaacacc ctcggccgga tcagagacgc ttttcgattt
     3481 ctatgcgcgc ctgatcaagg gcgaggcgtc atgtgaactt ggagaccacg ctgtgttttg
     3541 acgcggcgta gatcagtggt gcgctccagg gatttgcgtc aagactggcc tccggctcgg
     3601 cgagaagctt gctaacgtca gctgaagcgt cagcgccaat acccacgatc cggcatcgcc
     3661 tctaccggct gctccagagt tccacaagct tgccatccat gccaagttta tgtagatgaa
     3721 accaataaga accttggttc cgccaagaca cgcaagcggc acccaaggac ttatcctagc
     3781 catcaatcct cgcagtcttc gcccttgcta cctagacgca gagacagctg gaatggcgta
     3841 ttctaaaaag gagcagttta tgcgctt
//
LOCUS       DATMGX010000431         2517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_78721, whole genome shotgun sequence.
ACCESSION   DATMGX010000431 DATMGX010000000
VERSION     DATMGX010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78721"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VNR65_06955"
     CDS             complement(<1..761)
                     /locus_tag="VNR65_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HWJ18447.1"
                     /translation="MPHAIRIHAYGGPEVLKWEEVEVGEPGPGQAKIKQYAAGINYID
                     VYHRTGLYAQPSFPFTPGSEGSGQVIAIGPDVTEVAVGDRVAYAGAIGGYAEERLIAA
                     GRLVKIPDTITYETAAAMMLQGMTVRYLIKKTYHVTKDSVVLWHAAAGGVGLIACQWV
                     SSIGATIIGTVGSEEKAHLAKSHGCTHTINYRTEDFVPRVKEVTGGRGVDVAYDSVGK
                     DTFPGSLECIRPFGLWASFGNSSGAVPPFDIGLLA"
     gene            1371..2270
                     /locus_tag="VNR65_06960"
     CDS             1371..2270
                     /locus_tag="VNR65_06960"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HWJ18448.1"
                     /translation="MIQITYLSTATDPMSSHDLLSLLKECRESNASCGVTGMLLYCNG
                     TFLQVLEGAEKVVEEILDKIRKDSRHGNMQILDRKTIERRQYSDWSMGFKRISNRELQ
                     NVEGLREFGEKNFTSDYLVQHSPIVQSLMNHFRKERFKTIGHNELGVDEDDPMINVLH
                     RIIRGAVRVLAVLMVVTILWGVVDVVTVLYTQVFRPSYEEWQVRDIIVTFGAFLAVLI
                     AIEIFLNITLYLRDDVVHVKLVVATALMAIARKVIIFDFNKIAPMYILATGAVVLALG
                     IVYWLMDRKTILGEGSGRRISTD"
     gene            2311..2496
                     /locus_tag="VNR65_06965"
     CDS             2311..2496
                     /locus_tag="VNR65_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18449.1"
                     /translation="MTGYPEAITPLNAALTGYFPNLPAVEGLRDIVVAMCNTLSMTMR
                     DSASPRNLEEIGHGQHC"
BASE COUNT          547 a          686 c          721 g          563 t
ORIGIN      
        1 tgcgcgagga ggccgatgtc gaaaggcggc accgcgcctg atgaattgcc gaagctcgcc
       61 caaagcccga acggcctgat gcactcgagc gatcccggaa aggtgtcctt gccgaccgag
      121 tcgtaggcca cgtccacgcc gcggccaccg gtaacctcct tcacgcgggg cacgaaatct
      181 tcggtccggt agttgattgt gtgcgtgcag ccatgcgatt tggcgagatg cgctttctcc
      241 tcggagccga cagtgccgat aatcgtcgcg ccgatcgatg acacccattg gcaggcgatc
      301 aggccaacgc cgcccgccgc ggcatgccag agaacgacgc tgtccttggt gacgtggtag
      361 gttttcttga tgagatagcg caccgtcatg ccttgcagca tcatcgccgc cgccgtctca
      421 taggtgatgg tgtccggtat tttcacgagc cggcccgctg cgatgagccg ttcctccgca
      481 tagcctccaa tcgccccggc ataagcaacg cggtcgccaa ccgcgacttc ggtcacgtct
      541 ggccctatcg cgatcacctg gcccgagccc tcgctgcctg gcgtgaaggg aaaggacggc
      601 tgcgcgtaaa gtccggtacg gtgatagacg tcgatgtaat tgatccccgc ggcgtactgc
      661 ttgattttgg cctgccccgg tccgggctcg ccgacttcca cctcttccca cttcaggact
      721 tcggggccgc cataggcgtg aatgcggatc gcgtggggca ttctgaacct cttcttcgaa
      781 gggcggatta gcgacgccct gagtttttag gcactttgcc acaaaagttt tggttggcaa
      841 cggtttgtgg ccgtgtccag gtccctaacc ccgaaagtgg tcttgcgttg cgaaaagact
      901 tggaggttcg tgtgagagga ttggcagtcg gacaataaga aggccgtgag gactttgtcc
      961 ccacggcctt ctcatctaat gagcaaaggc gacgctaacc gccggtatct cagaggacgt
     1021 atggcaccag cggtgcaagc gtgatggccg tggccaatgc gacagcggac cattccttga
     1081 cagtaaaatt acgaaacatc gccatttgac agctccagtc tgaagttcaa ctgacctcat
     1141 tacggtgcac aggatcggct ggatggtttt tactggataa catatacttg agataaacgt
     1201 gaaagaagcc aacgcatact tgaactcggc gtgatagagc gagcacgggc gttgttcaac
     1261 actcttgaaa ttggtagcct tggcgttgcg gtttcgaccc ccaacgatca tgtcgttaaa
     1321 gctcataggc cccgacgcta tcttggctat tccgtcagat tcgagagata atgattcaaa
     1381 ttacgtacct cagtactgca acggacccca tgtccagcca tgacctgctc agtctcttga
     1441 aagaatgccg ggagagcaac gccagttgcg gcgtgacagg gatgcttctt tattgtaacg
     1501 gcacttttct gcaagttctt gaaggcgctg aaaaagttgt cgaggagatt ttggataaaa
     1561 ttcgcaagga ctcccgccac ggtaatatgc agatactcga tagaaagaca atcgagcggc
     1621 ggcaatactc cgactggagc atgggattca agcgcatctc taacagggag ttgcagaatg
     1681 tcgaaggctt gagagagttc ggcgagaaga atttcacgtc cgattatctc gtacagcata
     1741 gcccgatcgt tcaaagtctc atgaaccact tccgcaagga aaggttcaaa accatcggcc
     1801 ataacgaact cggcgtcgat gaggacgatc cgatgatcaa cgtgctgcat cggatcatcc
     1861 gcggcgccgt gcgcgtgctt gcggttttga tggtggtgac catcctatgg ggcgtggttg
     1921 acgtggtcac ggttctttac acgcaggtgt tcaggccgtc ctacgaggaa tggcaagtcc
     1981 gtgacatcat cgtcaccttc ggtgcgtttc tcgccgttct catcgcgatc gagatttttc
     2041 tgaacatcac gctctacctc cgggacgacg tcgtgcacgt caaactcgtt gtcgcaacgg
     2101 cgctgatggc gatcgcccgg aaggtgatca tctttgattt caacaagatc gcgccgatgt
     2161 acatcctggc gacaggcgct gtcgttctgg cgcttgggat cgtctattgg ctgatggacc
     2221 gaaaaacgat cctgggcgag ggtagtggcc gtcgcatcag caccgattga ttttcgatgc
     2281 cgggctccat ggatccgggg gcgagaaacg atgacgggtt atcccgaagc aatcacgccc
     2341 ctgaatgcag cattgactgg gtattttccg aaccttcccg ctgtcgaagg attgcgcgat
     2401 attgtcgtcg cgatgtgcaa cacattgtcg atgacaatgc gagacagtgc atcgccccgc
     2461 aatcttgagg agatcggtca tgggcagcac tgctgacagg atcaaagaca ttgggcc
//
LOCUS       DATMGX010000432         2220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_74672, whole genome shotgun sequence.
ACCESSION   DATMGX010000432 DATMGX010000000
VERSION     DATMGX010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74672"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(912..1799)
                     /locus_tag="VNR65_06970"
     CDS             complement(912..1799)
                     /locus_tag="VNR65_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ18450.1"
                     /translation="MSAFVAVVEAGGFSAASRKLGMPLATISRKVSELEEQLRVQLLV
                     RSTRNVSLTENGQQYFASCRRLLDELGEAERQVSGEYRAPKGGLVVSAPIVFGRLYLT
                     PIIVDFLKAYPEVHIELRLADSIAHLLEEQVDIALRIGELPDSGIIAARAGEIRHVVV
                     ASPAYLKERGTPAHPRELSAHDCVTFLALHAPDEWTFKLGKRNEKYPVQSRLAVSTAE
                     AAADAAIAGLGITRLLCYQVSKAIAERSLTLLMRDHEPTPLPVHLVYTGGRLIPQKLR
                     AFLDFVLPRLKAKLVFDPQ"
BASE COUNT          510 a          612 c          636 g          462 t
ORIGIN      
        1 tcggcttcat cttcgttccg ggcaaaaaca cgtggcacgc cgtcggcaag cggccgctgc
       61 acggcgcggt gcgaaaatcg ctgatcgtga attatgtcac ctccgcatgg cgggagacgt
      121 ccgagctgtc gtagcggaac ccggcgttgc gatgaactgc tggccaaaac gagttttgca
      181 tgcacattgc gcaagacggc atctgtcttc atcctccgga tcggctactt ctttgcgtgt
      241 tcagccggaa tgcttcagcc gtggatcata ggaaaaagct ccttgccggg aggttaatgg
      301 agctaagctg ttgataagac acaaaaaaag cgaaagcaat aggcgcggcg tcggcagcag
      361 aaggagctgt caaggttaac ggtctggtcc aaaaacataa ttaacggatt tcttcgcgcc
      421 gcgctttttc gcaaaagaac gcatcttttg gcgcaaattc aggccggaca taattccggc
      481 ggcccggaat taaccataag gagttaattt aaccccttgt cgcaacgcta tgttcaccat
      541 atcgggccta aattgcgcct ctatcataca acggctagcg tttttgcata cattgtcagg
      601 ttgcgcttga cgataatggt ccaaaagtcc cgctgccgcc tcatttcgcg cctctgcgaa
      661 gcaattgtcc caagcacggc ctgttcagtt tcgccaacga atgaagaaga ccgttgcaaa
      721 cggttcctcg cgagcgatgg gcggaaacct cccctgaaac gatctccgga ttaggccgat
      781 aacggaccaa gtgccttcac aaactctttt agattgatgg gaccgctttt ggatcctttt
      841 gcgggctacc acggattgac cttcagacgg tgaaggtggg ccacatgcgg aagtatcttc
      901 aacgctggaa tttattgtgg atcgaacacc agcttcgctt tcaggcgcgg cagaacaaaa
      961 tcaagaaacg cgcgcagctt ttgcggaatg aggcgtccac ctgtatagac gaggtggaca
     1021 ggcagcgggg ttggctcatg gtcgcgcata agaagcgtca agctccgctc ggcaatggct
     1081 ttcgacactt gataacacaa gagccgggtt atcccgaggc cggcaatcgc ggcgtcggct
     1141 gcggcctcgg ccgtactgac tgcaaggcgc gattgaacgg gatatttctc gtttcgcttg
     1201 ccaagcttga aggtccattc gtccggcgcg tgcagcgcca ggaaggtgac gcagtcatgc
     1261 gcggacagct cacggggatg ggccggggtt cctcgttcct taaggtatgc gggacttgcg
     1321 acgacgacat ggcgaatttc cccagccctg gcggcaatga tgccgctgtc gggcaactcc
     1381 ccgatgcgca gcgcgatgtc gacctgttct tcgagcagat gggcgattga atcggcgagc
     1441 ctgagttcga tatggacctc gggataggcc ttcagaaaat cgacaatgat gggcgtaaga
     1501 tagaggcggc cgaacacgat cggagcgctc accacgagtc cgccttttgg cgcgcggtat
     1561 tcgccggaaa cctggcgttc tgcttcgccg agttcgtcga gcaggcgccg gcaggaggca
     1621 aagtactgct ggccattttc agtcagtgaa acattgcggg tcgagcgcac caagagctgc
     1681 accctcagct gctcctcaag ctctgaaacc ttgcggctga tagtggcaag cggcatgccg
     1741 agcttgcgcg acgcggccga gaaaccgcca gcttcaacca cggcgacgaa cgctgacatc
     1801 gactcaaagc gatccattag ctttccgtaa tttggaatag tctgtctcga tattgccaga
     1861 tattcggcaa tttcgaaagg gcgtacattt tcgttcatgg agccggaccc cacggcactc
     1921 aagcgaaaaa ggaaaaaacc gatgattatg aaagtcgtga gctcagctct cgccgccgcg
     1981 atcgccgcat ctattggaac cgccgctgtg tacgcgaaag aaccctctgg ttaccggact
     2041 gcggcgcacc gcctgcacca tgaacatgcg acgcggtttg cgggtcactt ccacccatac
     2101 gcaagccagc tatacctcaa cgtgtgggga tcgcgtgtgt accttgaccg cggtggatat
     2161 tgaggccggg gcgaacccgg tgcaagagcc ggaccccacg gcactcaaac gaaaaaggaa
//
LOCUS       DATMGX010000433         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_43785, whole genome shotgun sequence.
ACCESSION   DATMGX010000433 DATMGX010000000
VERSION     DATMGX010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43785"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VNR65_06975"
     CDS             <1..1116
                     /locus_tag="VNR65_06975"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HWJ18451.1"
                     /translation="GPPMRPEPLPPGSTIGIFGAGQLGRFLAMAASKLGLRTAIYAPE
                     EDSPAFQVASQRWLSSYSDENALEAFAKACDVINFEFENVPAATLEIAARHSCVRPTV
                     RAAEIAQDRISEKRFLSGIGVPTASYVAVETEDDLPAAMSVLDKAGAGILKIARQGYD
                     GKGQARVSSEAELRSAFLNFAAPCVLEGLVRFSTEISVIAVRSAGGDVFFYDCPENTH
                     SGGILRKSAVPASCSERQQLLAQEMTARIATALDYCGVMGVEFFVMPEGSAEPLVVNE
                     IAPRVHNSGHWTMEACAISQFENHIRAIAGWPLGSVARHSDAIMTNLIGEEALDWRSI
                     LEKDPGASLYLYGKSDIRAGRKLGHVTQISTSQKEGG"
     gene            1239..1469
                     /gene="rpsU"
                     /locus_tag="VNR65_06980"
     CDS             1239..1469
                     /gene="rpsU"
                     /locus_tag="VNR65_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWJ18452.1"
                     /translation="MQVHVRDNNVDQALKALKKKMQREGIFREMKLRNYYEKPSEKRA
                     REKAEAVRRARKLARKKAQREGVALPGRTAAR"
     gene            1617..>2426
                     /locus_tag="VNR65_06985"
     CDS             1617..>2426
                     /locus_tag="VNR65_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ18453.1"
                     /translation="MTAGGLFLAIVALAACETSGTSEQRAADQLANPTADQKNNIASI
                     TAVLAKNPRDGNALNLRGTAFGQAGEYEKAISDFSAAIQINPQFYQAYNNRALIFLRV
                     ARYDAAMSDYNQALAIKPDYAAAYVGRGNVYKEQRNYPLAIADFAKAIELKPDDASPY
                     FSRGLIYQAMNEHRNALDDLTVAVNYRPDAADPHYARGMSYMATQDYKKAFDDFHVAG
                     TNKVNNFEAWAYAGQAAERLGDRAEAAQAYRKALQINSSSQLAYEGLKRVGG"
BASE COUNT          548 a          722 c          694 g          462 t
ORIGIN      
        1 ggcccgccga tgcgacctga gccgcttccg ccgggctcta ccattggcat tttcggcgca
       61 ggccaactcg ggcgttttct cgcgatggcc gcctcgaagc tcggcctcag aaccgcgatc
      121 tatgcgcccg aagaggacag ccctgcattc caggtggctt cgcagagatg gctgtcatcc
      181 tatagcgacg aaaacgcgct tgaagcgttc gcgaaggcct gcgacgtcat taacttcgag
      241 ttcgaaaacg tccccgccgc gacgctcgaa attgcggctc gccacagttg cgttcgcccc
      301 accgtccgcg ccgccgaaat cgcgcaggac cgcatttcgg aaaaacgatt cctgtcgggg
      361 atcggcgtgc ccaccgcgtc ctatgtggcg gtcgagaccg aggacgatct gcccgccgcc
      421 atgtcggtcc tcgacaaagc gggtgccggc attctcaaga tcgcgcgcca aggctacgat
      481 ggtaaaggcc aggcgcgcgt gtcttccgaa gcggaactcc gttctgcatt cctgaatttc
      541 gccgcgccgt gcgtcctgga gggtctggtt cgcttctcga ccgaaatctc cgtcattgcc
      601 gtccggtcgg ccggtggcga cgtcttcttc tacgattgcc ccgaaaacac ccactcagga
      661 gggatcctcc gcaagagcgc ggtcccagcc tcatgctccg agcgccagca attgctggcg
      721 caggagatga cggcgcgcat cgcaaccgcc ctcgactatt gcggtgtcat gggcgttgaa
      781 tttttcgtta tgccggaggg gagcgctgag ccgctggtcg tgaacgagat cgcgccgcgc
      841 gtgcacaatt ccgggcattg gaccatggag gcctgcgcca tcagccagtt cgagaatcac
      901 attcgcgcta tcgcaggctg gccgctcgga tctgtcgcgc ggcactccga cgcgatcatg
      961 acgaatctca ttggcgaaga agcactcgat tggcgttcca ttctcgagaa agacccaggc
     1021 gcgtctctct atctctacgg aaaaagtgac atcagagccg gccgcaagct tggccatgtg
     1081 acccaaatat caacctcgca aaaagaaggc ggataatcca caccctcgat aagcggaaaa
     1141 tggacatctc tgcttccgac tgctatattg accgtgcaag agcctgcatc tgttttgcgg
     1201 gcttttattc attccagaaa cagaaagaag gagccagcgt gcaagtccac gttcgtgaca
     1261 acaatgtcga tcaagcgctc aaggcgctca agaagaagat gcagcgcgaa ggtatttttc
     1321 gcgagatgaa gctgcgcaac tattacgaga agccctccga gaaaagagcg cgcgaaaaag
     1381 ctgaggctgt ccgccgggct cgcaagctcg cgcgcaagaa ggcgcaaagg gaaggcgtag
     1441 cacttccagg tcgtacggct gctcgataag agccacaaac gctgttccac agtcgtggat
     1501 agcatcggtg gttcacctga ttgtggccgc attcccggcc caaagtcgaa agcgtaagag
     1561 gaaattttac gccggtggat cgcgcattga caacggggca gactatgaga cgagcgttga
     1621 cagcgggtgg gctgttcctc gccatcgttg cgcttgcggc ttgcgaaacg tcggggacaa
     1681 gcgagcagcg agcagcggat caactggcta atccgactgc ggatcagaag aacaacatag
     1741 catcgattac ggctgtgctc gccaaaaacc cacgcgatgg gaatgccctt aacctgagag
     1801 gcacggcgtt cggccaagct ggcgaatatg aaaaggccat ctcggatttc tcggccgcaa
     1861 tccaaattaa tccgcaattc tatcaagcct acaacaaccg cgctctcatc tttctgcggg
     1921 ttgcccggta tgatgccgcg atgagcgatt acaatcaggc cctcgccatc aagccggatt
     1981 acgctgccgc ctatgtaggc cgcgggaatg tgtacaagga acagagaaat tacccgttgg
     2041 cgattgcaga ttttgcgaaa gcgatcgagc tgaagccgga tgacgcctcg ccttacttca
     2101 gccgtggctt gatctaccag gccatgaacg aacatcgcaa cgcgctcgac gaccttaccg
     2161 tggctgtcaa ttaccggccg gacgccgccg atccgcacta cgcccgcggc atgagttaca
     2221 tggccacgca ggattataaa aaggccttcg acgatttcca cgttgccggc acaaacaaag
     2281 tgaacaactt cgaagcctgg gcttatgcag gtcaggcagc cgaacgcctt ggggatcgcg
     2341 cagaagccgc acaggcatac cgcaaggcgc ttcagatcaa ctcctcaagc cagctcgctt
     2401 atgaagggct caagcgtgtt ggcgga
//
LOCUS       DATMGX010000434         7530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_356401, whole genome shotgun sequence.
ACCESSION   DATMGX010000434 DATMGX010000000
VERSION     DATMGX010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356401"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VNR65_06990"
     CDS             <1..190
                     /locus_tag="VNR65_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18454.1"
                     /translation="MPPSDIAKKSHFEHFDTKAEMHLLLFGFVGSFRLFGKVVRDTLL
                     AIDTSCASLEPRPNSVLF"
     gene            complement(221..1372)
                     /gene="nrfD"
                     /locus_tag="VNR65_06995"
     CDS             complement(221..1372)
                     /gene="nrfD"
                     /locus_tag="VNR65_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWJ18455.1"
                     /translation="MIPWTILIVVYPYLTGLVAGAFTISSLYQVFGFQQLRPVAHFAL
                     LVSLCCMVVVPIPLLLHLGHPERAFNAMITPHTTSAFAIFAYAAAFYVILLILETWFV
                     FRPQIVQTAKTTKGPLGWVYRVATLGSDDLSEKALSYDKKWLFALAIIGIPGAHGLHG
                     YVGFVFGSLKSREWWSSDLMPVIFLFSAIISGTALVIVLYVVSCRLRKVPVDLACLKV
                     MAYSLWGFMMFTLLLEGLEFASMVYRGREGIDVIMSFVKGPLLVPFFILQFGIGSVLP
                     LAVITFLIWRGTSGPTLVVAITTSACLVLLAVLMMRYNVVIGGQEIAKTGKGLLAYRP
                     PIFAREGLFVAGCVLVLPFVLLSILVRFLPPWHEAESALAKGMPSVIAH"
     gene            complement(1429..2289)
                     /locus_tag="VNR65_07000"
     CDS             complement(1429..2289)
                     /locus_tag="VNR65_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWJ18456.1"
                     /translation="MEDKEKLEGQQTGRREFLSCLGSALACSAAAAAAGAITTGTVAT
                     TAAEAAVTEVPPNPAIPGYDWTQHQWAFGVDATKCIGCLRCVEACKKENNVPQDAHHF
                     RTWVERYVTLEGEETARVDSQADPVNIAASGSESEYRFADRYRDAKVELAFFVPKLCN
                     HCTHPACVQVCPTGATYKTEDGVVVIDHTYCIGCQYCVQACPYGARYFNEETGVTDKC
                     TWCYHRITKGLQPACVEVCPVGARIFGDRNDKQSAISQFIQNNRVSVLKPETGNAPNV
                     FYIGIDKDVS"
     gene            complement(2329..3369)
                     /locus_tag="VNR65_07005"
     CDS             complement(2329..3369)
                     /locus_tag="VNR65_07005"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ18457.1"
                     /translation="MPRHIFRLILVILVCVVVGYGAKRFFTVNSFYEYGHYRGDSVAD
                     IASDKPKFKGVGYCASCHAKQTAEWSNGVHNSSDIGKIVKCEVCHGAGGERDVLGPFE
                     ASATGADHPNNLKMVVPTDTRKLCTFCHERMTGRPLQQRQIVVADHAGTQQCIVCHNP
                     HSPKLNLTPVAEAAAQAGDPIVGKAKAAACAGCHGAEGVSRNLPGPSLAGQNAAYFAE
                     ALKAYGTGGRNNPMMSPAAQGMNAEDTGHLAAYFSGLKCEAAPASDAQAVSAGKAIAS
                     KCTACHGADGRAGNGAWPNLAGQSKDYLVNAIKAYKGGARNNGMMAGIVKDLNDSDAE
                     SVATYYASAACK"
     gene            complement(3861..4652)
                     /locus_tag="VNR65_07010"
     CDS             complement(3861..4652)
                     /locus_tag="VNR65_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18458.1"
                     /translation="MAEQPPLHRLYRHYSELPYSQRVLYTMTLLVLGLGYMFALIYVF
                     HTYAGRGTGNKMMLSYEDLVVAYSGSGHGSRLESALQGPMSNMLPSDETKAIIAWVQQ
                     GSDHASYDKSVKPILDKRCMGCHDGSNPHLPNLSSYDNIKKVTEQDTGTDVFTLVRVS
                     HIHLFGLTFIFFIMGLMFTHAYVRPVWLKCMIVALPYCAIVMDVSSWYFTKLFHPFAW
                     VVLFAGALMGGCFAFMWLVTMYQLWFSPPPAMVAERVGGDTAIIG"
     gene            complement(4759..5067)
                     /locus_tag="VNR65_07015"
     CDS             complement(4759..5067)
                     /locus_tag="VNR65_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ18459.1"
                     /translation="MKRMLLNIGIAALLGIGGAHAAGDAAAGKAKSAACAGCHGPNGE
                     GIAPNPALAGKSEEVLVQALKDYKSGKRDNAVMKGMVGALTDGDMTNLAAYYASLKAK
                     "
     gene            complement(5126..6313)
                     /locus_tag="VNR65_07020"
     CDS             complement(5126..6313)
                     /locus_tag="VNR65_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ18460.1"
                     /translation="MFLAGACFEGASAQPRINADDGPELRTLYANSQDVAEGKRIVES
                     SCAGCHGANGVSESPGVPHLAGQRPAYLYLELKAYQAGVRGESAMNNVVRPLNDDALF
                     KAAAYFASLDPPQTSAANSANATAPDPVQAGKTAAAGCVGCHGDGGISKTPGMPSLVG
                     LDPKYLVAAMKAYKSGQRKNDMMKSMLASVTDASMNNIALYFGLQKPARAQTPAAGDK
                     AAGETAAAACAGCHGSDGVSGNPATPSLAGQDASYIASALQGYKQGARSDETMKGLAA
                     SLDDSAVKNVAAFYAGQEPKQPNVRKPQTAEEWAERCDRCHGINGNSADPRLPALAAQ
                     RLDYLEKVLQAYRTGARKSPQMAAMSDVLTDQDITNIAAYYTRQKARGVVFVILQSKS
                     GTK"
     gene            complement(6590..6814)
                     /locus_tag="VNR65_07025"
     CDS             complement(6590..6814)
                     /locus_tag="VNR65_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18461.1"
                     /translation="MNVKVLGAVVAALAVGGVLTIAAPKPAAALPAYAAATGKACGAC
                     HANPAGGGPRTAFGEAFAANGHKLPGKKPK"
     gene            complement(7264..>7530)
                     /locus_tag="VNR65_07030"
     CDS             complement(7264..>7530)
                     /locus_tag="VNR65_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18462.1"
                     /translation="WKLFEANRLTEPLIWYVSYRSNISPKRDDGERKLTRATRKFKDE
                     AEARQFAEQIIEKGWSAIAGTLNPHTPKKTIASTQILDWIAGKH"
BASE COUNT         1575 a         2251 c         2109 g         1595 t
ORIGIN      
        1 gatgccgcct tcagatattg ctaaaaagtc acacttcgaa catttcgaca cgaaagccga
       61 aatgcactta cttcttttcg gtttcgtcgg cagcttccgg ctctttggga aggtggtgcg
      121 cgataccctt ttggcaatcg atacaagttg cgccagcctt gagccgcggc cgaacagcgt
      181 ccttttctga ggaaatctat tccggtcgga cgctcggccg ttagtgggca ataaccgacg
      241 gcatcccctt cgccaaagct gattcggctt cgtgccacgg cggcagaaag cgaaccaata
      301 tcgacagcaa gacgaatggc aggaccagca cgcagcccgc gacgaataaa ccttccctgg
      361 cgaaaatcgg cggccgatat gcaagcaacc ccttgcccgt cttcgcaatt tcctgcccgc
      421 cgatcaccac attatagcgc atcatcagca ccgcgagcag gaccaggcat gcgctcgtgg
      481 tgatggctac gaccagcgtc ggtccgctcg tgccgcgcca aatgaggaag gtaattaccg
      541 cgagcggcag caccgaccca atcccgaatt gaaggatgaa gaacggaaca aggagcgggc
      601 ctttgacgaa cgacatgatc acgtcgatcc cttcccggcc gcgatagacc atgctggcaa
      661 attccagccc ttccagcagc agggtaaaca tcatgaaacc ccacagcgaa taggccatga
      721 ctttcaggca cgccaggtcg accggaacct tgcgcagcct acacgacacg acatagagca
      781 cgatcaccag ggccgtaccg gagatgatcg cggaaaacag aaagatgacc ggcatgagat
      841 cggaggacca ccattcgcgg gacttgagcg aaccgaatac aaaacccacg tagccgtgca
      901 agccgtgagc gccgggaatg ccgatgatcg ctaacgcgaa tagccatttt ttatcgtaac
      961 ttaaggcctt ctcggagagg tcgtccgacc cgagcgtcgc cacgcgatag acccatccca
     1021 gcggcccttt cgtggtcttg gctgtttgca cgatttgcgg ccggaacacg aaccaggtct
     1081 ccaaaatcag cagaatgaca tagaaggcag ccgcgtaagc aaagatggcg aacgccgatg
     1141 tcgtatgcgg cgtgatcatg gcgttgaatg cccgctccgg gtgaccgagg tgcagcaaca
     1201 gcggtatcgg cacaacaacc atgcagcaca aggagaccag cagcgcgaaa tgcgccaccg
     1261 gcctcaattg ctggaagcca aatacctgat acaggctcga gatggtgaag gcgccggcga
     1321 ccaatccggt caggtaggga tagaccacga tgagtatggt ccacgggatc atcgtctcgt
     1381 tgggataaac gtagccggtg aatggagcgg agtgcactaa ggtttccatt agctcacgtc
     1441 cttgtcgatg ccaatataga agacattcgg cgcattgccg gtttctggtt tcagcacgga
     1501 aacccgatta ttttgaatga actggctgat tgcgctttgc ttgtcattgc gatcgccgaa
     1561 gatgcgcgca ccgaccgggc aaacttcaac gcaggcaggc tgcagtccct tggtaatgcg
     1621 gtggtaacac cacgtgcact tgtcggtgac gcccgtttcc tcattgaaat agcgtgcgcc
     1681 ataagggcaa gcctgcacgc aatattgaca accgatgcaa taggtgtggt cgatgacaac
     1741 aaccccatcc tcggtcttat aggtcgcacc ggtcgggcat acctgaacgc aagccggatg
     1801 ggtgcagtgg ttgcacagct tgggcacgaa gaacgccagt tccacttttg cgtccctgta
     1861 gcgatcggcg aaacgatatt cgctttcaga gccggaggcc gcaatattca ccggatcggc
     1921 ctgactgtcg acacgagcgg tctcttcgcc ctccaaggtc acgtatcgct cgacccaggt
     1981 gcggaagtga tgcgcgtcct ggggaacatt attttccttc ttgcatgcct cgacgcagcg
     2041 caggcagccg atgcatttgg tggcatcgac gccgaatgcc cattggtgct gagtccaatc
     2101 atatcccggg attgccgggt ttggcggaac ttcggttacc gccgcctccg cagcagtcgt
     2161 cgcgacagtt cctgtcgtta tagcacctgc cgccgctgct gccgcgctgc atgcaagagc
     2221 acttccgaga cagctcagaa attctcgccg ccccgtctgc tggccttcga gcttttcctt
     2281 gtcttccatt tgcgtatgct cctgggcaac tgtccgttcg gccaagtcct atttacatgc
     2341 cgcgctggcg taataggtcg ccacgctttc ggcatccgag tcgttcaagt ccttcacgat
     2401 tccagccatc atgccgttgt tacgggcgcc gcccttgtag gctttaatgg cgttgaccag
     2461 ataatcctta gactgcccgg caaggttcgg ccacgctccg ttgccggcgc gtccgtcggc
     2521 gccgtgacag gcggtgcact tcgatgcgat cgctttgccc gccgatacgg cctgcgcatc
     2581 cgacgccggc gcggcttcgc atttcagtcc cgagaaatag gccgcgagat ggccggtatc
     2641 ttcagcgttc atcccctgtg ccgccgggct catcatcgga ttgttacggc cgcccgtacc
     2701 gtatgccttg agggcttcgg cgaaatatgc cgcattctgt ccggcgaggc tcgggccggg
     2761 gagatttctg ctgacgccct ctgcgccgtg acagcccgca caggcagcag ccttggcttt
     2821 accgacgatg ggatcacccg cctgggccgc tgcctctgct accggcgtca gatttagctt
     2881 cggcgaatgc gggttatgac aaacaatgca ctgctgcgtg ccggcgtggt cggcgaccac
     2941 gatttgacgc tgctgaagag gcctccccgt catccgttca tgacaaaacg tgcaaagctt
     3001 tctggtgtcg gtcgggacga ccatcttgag attgttcggg tgatccgcgc cggtagccga
     3061 cgcttcgaac gggccgagaa cgtcgcgctc gccgccggca ccgtgacaca cctcgcattt
     3121 aacaatcttc ccgatgtccg agctgttgtg cacgccattg gaccattccg cggtttgctt
     3181 cgcatggcat gacgcgcaat accccacccc tttgaatttc ggtttgtccg aggcgatgtc
     3241 tgctaccgag tcacccctgt aatgaccgta ttcgtagaac gaattgacgg tgaaaaatct
     3301 cttcgcacca tatcccacca cgacgcagac caagattacc aggatcagcc tgaaaatatg
     3361 tcgcggcatg gcgttcccgt ttcttggggc tatccggtcg acatcgagat gtgtgtgatc
     3421 gaaatcgccg ccctgccgga atatcgtccg gcaagttcac ggcatcggat gcgcagtggg
     3481 ttcttcattg aagtccccct tctgtcggga gggcgcggga atcggttcct cgaaattaaa
     3541 ttagcgaaat gcggattcga gtaattgact caaatcaaat caccggtttt ccggtaacaa
     3601 taaagacttt tcaacttctt caatcggaaa aatactttcg cgagcgatgg cgccggagga
     3661 tcgcgcgcac ccggccggcc gctgcggcag ccgatcgtgc gggcctgagg ccggtcgagc
     3721 gggcaaatag aagcgccgcg aatatcgccg acagataggc catacctgtg ctgttagtgc
     3781 agaacctcgg gcaagcgctc tatcttcgca cttgcccgag catggttata agcaccgtta
     3841 cgctgctttg ccgacgtcac tcagccaatg atggcagtat cgccgcctac tctctcggca
     3901 accattgcag gcggcggcga gaaccaaagc tggtacatgg tgaccagcca catgaaggcg
     3961 aagcatccgc ccatcaacgc gccggcgaac aatacgaccc aggcgaatgg gtgaaacagc
     4021 ttggtgaagt accacgagct gacatccatt acgatggcgc aatagggaag cgcgacgatc
     4081 atacacttga gccagacggg ccgcacatag gcatgggtaa acatcaggcc catgatgaag
     4141 aagatgaagg tcaggccgaa cagatgaata tgagacacgc gaaccagcgt gaagacatcc
     4201 gttccagtat cctgctccgt caccttcttg atattgtcat agctgctcaa atttggcaga
     4261 tgcgggttac tgccgtcgtg gcaacccata caccgcttgt ccagaatcgg cttgacgctc
     4321 ttgtcgtagc tcgcgtgatc ggaaccttgt tgcacccaag cgatgatggc tttggtttcg
     4381 tcggacggca gcatgttcga cattggtcct tgcaatgccg attcgagacg cgagccgtgg
     4441 ccgctgccgc tgtaggcgac gacgagatcc tcgtaggaga gcatcatctt gttgccggtg
     4501 ccccgtccag cgtatgtgtg aaagacgtag atcagggcaa acatgtaacc caacccgagt
     4561 accagcagcg tcatcgtata caagacgcgc tggctatacg gcagctccga atagtgacga
     4621 taaagcctgt ggagcggtgg ctgctcagcc atgagctatt cctttaattc ttgcgggccg
     4681 taaatcagag gacgatggtc gccgcagcgt taagaagcgc gcccaatcgt ttgctaaagc
     4741 gaacatgctg gcgcgatatc actttgcttt gagcgatgcg tagtaagccg ccaaatttgt
     4801 catgtcccca tcggtgagag cgcctaccat tcccttcatg acggcgttgt cgcgcttgcc
     4861 cgatttgtag tccttcaggg cttggacaag cacctcttcg cttttgcccg ccaatgcagg
     4921 attgggcgcg atcccttcac cgtttgggcc atggcacccg gcgcaggcag cggacttggc
     4981 cttgccagcc gcagcgtcac cagcggcatg ggcgccgcca atcccgagta aagcggcaat
     5041 tcctatgttc aacagcattc gtttcattgt ctgctccttt tgaaaggtgt tgccatcgcc
     5101 gcggtgccaa gacgtatgtt cctgctcatt tggtacccga tttggactga agtatcacaa
     5161 ataccacccc gcgcgccttc tgccgcgtgt agtaggccgc aatgttggtg atatcctgat
     5221 ccgtcagcac atcggacatt gccgccatct gagggctctt gcgcgcgcct gtccgatagg
     5281 cctgcaaaac cttttccaga taatccaagc gttgcgcagc caaagcgggc aaccgtggat
     5341 ccgcactgtt gccgttaatg ccgtgacaac gatcgcacct ctccgcccat tcctcggcgg
     5401 tctgcggctt gcgtacgttc ggctgcttcg gctcctgacc agcgtagaag gctgcaacat
     5461 tcttgactgc gctgtcgtcg agcgacgccg ccaaaccttt catggtttcg tcgctgcggg
     5521 ctccctgctt gtagccctgc agcgcgctcg cgatatacga tgcatcctgt ccggcaaggc
     5581 tcggagtcgc cggatttccg ctcacaccat ccgacccgtg gcatcccgca caggcggctg
     5641 ccgccgtctc gccggcagcc ttgtcgccgg cagcaggggt ctgggcccgc gccggctttt
     5701 gcagcccaaa gtagagcgcg atgttattca tgctcgcatc ggtaacgctt gcgagcatcg
     5761 acttcatcat gtcgttcttg cgttgcccgc tcttgtacgc cttcatcgcg gcaacaagat
     5821 atttcggatc caatccgaca aggcttggca tgccgggggt cttgctgatt ccgccgtccc
     5881 catggcatcc cacgcagccc gccgctgcgg tcttgccggc ttgaactgga tcaggtgcgg
     5941 tagcgtttgc gctatttgct gcactggttt gcggagggtc taggctggca aagtaagcgg
     6001 ccgctttgaa cagcgcatcg tcatttagtg gcctgaccac gttattcatt gcgctctcgc
     6061 cgcggacgcc agcctggtag gccttcagct cgagatacag gtaggctggg cgctgaccgg
     6121 caagatgtgg cacgccggga ctttcactga cgccattcgc gccatgacag ccggcacagg
     6181 aagattccac gatgcgcttg ccttccgcga catcttgcga gttcgcatac agggttcgca
     6241 gctccggacc gtcgtcggca ttgattctcg gttgagcgga cgcgccctcg aaacaggctc
     6301 cagcgagaaa cacgccggca atgactcccg cggcggccag ggacgggcaa cgaaggcaag
     6361 cactcatctg tcggagaaaa acccggaatc gtgctcggca cccgttcaga gaagttgcgt
     6421 cgattgatcc ttgcatccta tccccatcct gcggctcacc tagtttagtg caacgagccg
     6481 attccgattc cgctatcgga gcctgtaaat tttatcgcaa gcacaagaag ggactcacaa
     6541 tatcaaaata gtttgcaaat cccttttgtc tgggaaacct agccttgaac tatttgggtt
     6601 ttttacccgg cagcttgtgg ccgtttgctg caaatgcttc accaaatgcg gtgcgaggac
     6661 cgccgccggc cgggttcgca tgacacgcac cgcaggcttt tccggttgcc gcggcgtagg
     6721 ccggcaatgc cgccgcaggt ttaggggcgg cgatcgtcag caccccgcca actgccaacg
     6781 ctgcgacaac agctcccagg actttaacat tcatcttgca tctccgttga tgggaacgaa
     6841 cgcaaaaaca agtaagcgat tttcggcgca aaagatttga tctcgatcaa caacatcgct
     6901 cgaatagcgc ggccattcaa gactaattga accatcgata caacttcctt taccgcgcta
     6961 tggtgacagg cagatgagcg cggtggctcg gatcgacgag caggatgcgc caatcgggaa
     7021 gccgcacgcc atcgcaattc cgggagccgc tccgagagcg gccggctcgc agccaaacca
     7081 gttcagcaac cccatcgccg ctgcccgctc gcaagagcga ccccgcgcgg ttcgagcgcg
     7141 tttgacgccc taccccggcg gatgtcgatc tgacgggacg gaagctcggt ctggtcggtc
     7201 gggtgtccct tccgggcgcg caatgcacgc aagcgggcat ataccgggcg ccgcaagatt
     7261 ctttcagtgc ttgccggcga tccaatcgag aatctgcgtg gaagcgatgg ttttcttcgg
     7321 cgtatgggga ttcagggtcc cggcaatcgc gctccagccc ttttcgatga tttgctcggc
     7381 aaattggcgc gcttcggcct catccttgaa tttgcgagtc gcgcgcgtca acttgcgctc
     7441 tccgtcgtcg cgcttcggcg aaatgttcga gcggtaggaa acgtaccaga taagcggctc
     7501 ggtcaagcga ttggcctcga acaatttcca
//
LOCUS       DATMGX010000435         2243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_76208, whole genome shotgun sequence.
ACCESSION   DATMGX010000435 DATMGX010000000
VERSION     DATMGX010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76208"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VNR65_07035"
     CDS             complement(<1..763)
                     /locus_tag="VNR65_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWJ18463.1"
                     /translation="MASGRFQDDTGKSWPMRRFRPAGAIIGAGLLSVSFLAGLPAAVL
                     FADSAAAEEMRGQNFAPVSFADLADKVRPAVVSVNVKSRGAVGDVSNRDIPVPDLPPD
                     SPLRDFFDQFKHGQPERRPTRAQGSGFLISSDGYIVTNHHVADKADEIEITFENEEKY
                     KAKIVGSDSRTDIALLKIESGKKFNNWLEFEDSPPRVGDWVLAVGNPFGLGGTVTAGI
                     VSAAGRDIGSGPYDFLQIDAAVNRGNSGGPAVDLRG"
     gene            1214..1321
                     /locus_tag="VNR65_07040"
                     /pseudo
     CDS             1214..1321
                     /locus_tag="VNR65_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            1592..>2243
                     /locus_tag="VNR65_07045"
     CDS             1592..>2243
                     /locus_tag="VNR65_07045"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWJ18464.1"
                     /translation="MSLSNEATDRAMMARCIELSTMAVKEGEYPFGSLIALDGRIIAE
                     ATNRTVRENDVSRHAEVLALSQAQQVVGRLDLQRATLYSNVEPCAMCSYCIREAWVGR
                     VVYALTSPIMGGASKWNILRDADISSRVPIFGPAPEVVSGVLVGKVQEAWRAWNPLAW
                     EMVRMRGVLTAHEVPEEESVQVLPRHRRSAWHYAISAFMMFSKKAKPVPVPAARQQG"
BASE COUNT          492 a          630 c          678 g          443 t
ORIGIN      
        1 tgccgcgaag atcaaccgcg ggtccgcccg aattgccgcg gttcacggcg gcgtcgattt
       61 gcaggaagtc ataaggcccg gaaccgatat cacgaccggc agccgaaacg atgccggcgg
      121 tcacggtgcc gccaaggccg aacgggttgc caacggccag cacccagtcg ccaacgcgag
      181 gcgggctgtc ctcgaactcg agccagttat tgaatttctt gcccgattcg attttcaaaa
      241 gcgcgatatc ggtgcggctg tcgctgccga cgatcttcgc cttgtacttc tcttcgttct
      301 cgaaggtgat ctcgatttca tcggctttat cggcgacgtg gtggttggtc acgatatagc
      361 cgtccgaaga aatcagaaag ccggacccct gtgcgcgcgt cggccggcgc tcgggctggc
      421 cgtgcttgaa ctggtcgaag aagtcgcgca gcggtgaatc cggcggaagg tccggaacgg
      481 ggatatcgcg gttcgagaca tcgccaaccg caccgcgaga cttcacgttc acgctgacga
      541 cagcgggccg gaccttgtcg gcgagatcgg caaaacttac gggagcgaaa ttctgcccgc
      601 gcatttcttc cgcagcagcg ctatctgcaa aaagaaccgc cgctggaagc ccggcgagga
      661 aactcacgct taacaaacct gctccgatga tggcacccgc cggccggaaa cgccgcatcg
      721 gccaactctt gcctgtatca tcctggaaac gtccggatgc catcagcttc gtactcccct
      781 aatggaattg atctggagat catataagaa accgaatgtt acaaaccact tgccgcagaa
      841 ttaaaagatg gtaaggcgag cacttgtgcg cttctttttc caaagacggt tcttgacttg
      901 cggctctggc ttcctagcgc agtttattgc gcgaagcttt gctatttcaa gggtttaggt
      961 ttctcaaaca agagcgcgaa ggcgccataa gcttctgaaa cagacgtagc aggcctcatt
     1021 tgcatggtga tcaacgcaag gagtcaccat gataattgaa atcgagcaat ctggcgccga
     1081 gcgtcccgac gaagtccgac gccggctctt ggtgagcgga ctcacgggag cagcggcatt
     1141 ggcgatgtct cagaacgtgc aggcgcaaga aaaagcccca ggcgcaggac cgatcaaggc
     1201 ggggcgcgga accatgctca cgggaaaggt tgcggtcgtt accggcgcgg cgcgcggcat
     1261 cggccgggcc atcgccgtcg aactggcggc gaacggagcc gatatcgccg ccatcgatat
     1321 ttacggtttt gtgagcaccg catcgaacgc agcgccagcg acacaggaag accttgagga
     1381 aacgactcga caggtctgcg gactcggccg gcaatgtacg gcctggcttc agcccggctc
     1441 gcatttaatt tatacaggag cggctagaaa acgaagcggc aatagcttgc cgcctgttat
     1501 accgctagaa aaggctatat agtaattacg acgtgttagg atgagttgat cgcctgaccg
     1561 gctctgcaaa aaactatggg tgggggaccg aatgagcctg tcaaacgagg cgacagaccg
     1621 ggcgatgatg gcccgctgca ttgagctgtc tacgatggcg gtcaaagagg gcgaatatcc
     1681 gttcggctcc ctcattgcgc tcgatggccg gatcatcgcc gaggcaacaa accgcaccgt
     1741 gcgagagaac gacgtatccc gtcatgccga ggtgctcgcc ctttctcaag cacaacaggt
     1801 tgtcgggcgg ttggaccttc agcgggcgac gctctacagc aatgtcgagc cttgcgcgat
     1861 gtgctcctac tgcatccgcg aagcctgggt cgggcgagtc gtctatgctc tcacatcacc
     1921 aatcatgggc ggcgcatcca aatggaatat tctcagggat gcggatattt ccagccgcgt
     1981 tccgatcttc gggcccgctc cggaggtggt gagcggcgtt cttgtgggaa aagtccagga
     2041 agcgtggcgg gcatggaacc cattggcctg ggagatggtg cggatgcgcg gcgtattgac
     2101 cgcgcatgag gttccggagg aggagtccgt gcaagtgttg ccccgtcacc gacgatcagc
     2161 ttggcattac gcgatctccg cctttatgat gttttcgaag aaggctaagc ctgttccggt
     2221 tcctgctgca cgacaacaag gaa
//
LOCUS       DATMGX010000436         2857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_288825, whole genome shotgun sequence.
ACCESSION   DATMGX010000436 DATMGX010000000
VERSION     DATMGX010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288825"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(865..1818)
                     /locus_tag="VNR65_07050"
     CDS             complement(865..1818)
                     /locus_tag="VNR65_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18465.1"
                     /translation="MSVREFLGLPHTTGSLLVLTLAVVAGAAPPSYADVVTDWDAKAT
                     VAAPPGALGERELVIVDLAMFDAVNSIARRYPPYLVREERYGGTSGEAADASAAATAL
                     EKLRPQSAAEWKTELDGYLKGLSASHDAVEQGARLGTLVALRVFEPRAADGANGVDPY
                     RPRTQPGVYVPTATMVSSTWPTMRPIVLERPEQFRPGPPSRLRVPNGRRTTMYAVALY
                     DAYVAVFDAKYTYEFWRPITAIRNGDIDGNPATDIDPLWQPLDDTPMHSEYPCAHCIQ
                     SATAAALIEASGAMANLQEPILKSPKSAGRYPPLVVARSLQ"
     gene            2094..>2857
                     /locus_tag="VNR65_07055"
     CDS             2094..>2857
                     /locus_tag="VNR65_07055"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWJ18466.1"
                     /translation="MEQRDGRAGGQTNRLRRLWREGRCTLGAIATIPSVQTVQVMARS
                     GLDWIIIDMEHGPIDLAAAHAMIAATTGTPLVPLVRVGTGAAWHAKTPLDLGAMGICF
                     PMTTRHEDAVAAVRSVRYPPEGERYWGPFYAPLRWDVSMREYLDRANEEVLAIGTIEH
                     IDALDTVNSVVATAGLDLVFIGPGDLATSMGLKGRVDDPSVQSAIAKLEAQILASPVL
                     LGGVAHTAEQASAMIARGYKALVVGFDWSFLQRGIAA"
BASE COUNT          573 a          852 c          896 g          536 t
ORIGIN      
        1 gcgtggagaa aggcgatatt ggcggccacg tacaggccaa tgatcattag catgcctagc
       61 aacagcgccc gcggcagatt gcgctgtgga ttctgaacct cgccgccgat catcgcgacg
      121 ttagcccatc ccttgtaggc agccatggcg gcgagcgcag cagcactgac tgcgccaagg
      181 ccggaaagtc gccgggtgta ttctgcagaa agacgtactc gccgcctggg cgagggaggc
      241 gggcagccaa ctgtgaatac gtgatggcac cagccaggct tagcaatccc gcgccgaccc
      301 agacggccaa gatccacgac gaagagccca cccattgggc tatcgatgcg ggccgaatat
      361 aaatgccagt gccaaggatg ccgcccacta cagcggcaag cgcgtgccag cgcgttactc
      421 tgcgctgcag ccccacgtcg gagacgttgt tcgactggaa tgatggccgc tggcgaaggc
      481 acatgccatc aaatcgtgtc acggactctg accataccgg tcgctcgcca atggcacgaa
      541 taggtcagaa cttcgccttg cctatcgccg gcgaaaattg gcagtcttag attgctgatt
      601 cggaagggca gctttcctcg ccgttcagga gtaatgcaga aacgcaagcg gccaactgct
      661 gccgctcggc acgcgaaact gacgccggct aagcggccat tcgacccgca gtcatatcgt
      721 taccaaggcg agtgcgctgc tttggtttcg agctgtccga aaggcgtggc gacgtatcgg
      781 ccaatctccc ggcccatggc atcaccgacc cgggtggagg agcggtagtg aaatccagcc
      841 cagatgcgcg cattcgcaac ctctttactg aagggatcga gcgacgacca gcggtgggta
      901 acgcccggcg ctttttggac tcttaaggat aggttcctga agatttgcca tcgcgccgct
      961 ggcttcgatc aaggccgccg cggttgcgct ctgtatgcag tgagcacagg gatattcaga
     1021 gtgcataggc gtgtcatcga gcggctgcca taagggatca atatcggtcg ccgggttgcc
     1081 gtcgatatcg ccgttgcgga tcgcggtgat gggacgccag aactcatagg tgtatttagc
     1141 gtcgaatacg gcgacgtagg cgtcgtacaa cgcaacggcg tacattgtag tccgacgccc
     1201 attcgggact cgcaagcgcg acgggggccc cggacgaaac tgctccgggc gctcgagcac
     1261 gattggacgc attgtcggcc aagtcgatga aaccatcgtc gcggtgggca cgtacacgcc
     1321 gggctgcgtg cgcggccgat acgggtcgac accgttcgcg ccatcggcgg cacgcggctc
     1381 gaagacccgc agtgcgacca gcgtgccgag gcgcgctccc tgttccaccg catcgtgcga
     1441 tgcggacagc cccttcaagt aaccatccag ctcagtcttc cattcggcgg cgctctgcgg
     1501 acgcagcttt tcgagcgcgg ttgccgctgc gcttgcgtcg gcggcttcgc ccgatgtgcc
     1561 gccgtagcgc tcctcgcgga caagataagg cgggtatcgc cgggcgatgg aattcacggc
     1621 gtcgaacatg gcgagatcca caattaccag ttcgcgctcc cccagtgccc ccggtggcgc
     1681 ggccactgtg gccttcgcgt cccaatcggt gaccacatcc gcgtagcttg ggggagcagc
     1741 acccgcgacg acggcgagcg tcaggaccaa aagtgaccca gtcgtatgcg gcagtccgag
     1801 aaattcgcga actgacatga cgccactcct atgacaagtg acagtgaccc gagccctcta
     1861 gggtggcccc aaggcgcgcg gcgctaacaa ttgtcgctgc aggcgctgca atagtctgac
     1921 ccctccaagc gagttagtgc agcgttctga tgccgggtac cggccactcg caagtgaccg
     1981 gtgcggatca gtgacgtaga tgaccaatga ccacctgagt aatttcggtc agtgctgcat
     2041 caaacgaaat gtcttctata ctgtagttcc ttgattaggc ccgggaagct aggatggaac
     2101 aacgagacgg acgcgctggc ggccaaacca accggttgcg cagactctgg cgggaaggcc
     2161 gctgcacgct gggcgccata gctacgattc cgtccgttca gaccgtgcag gtgatggcaa
     2221 ggtccggctt ggactggatc atcattgaca tggagcacgg gcccatcgac ttggcggcgg
     2281 cgcacgcaat gattgcagcg accaccggta cgcctttggt tccgcttgta cgcgtaggga
     2341 ccggtgctgc atggcacgcg aagactcctt tggacctcgg cgcgatgggt atttgttttc
     2401 ccatgaccac gcgccatgag gacgctgtag ctgcagtgcg ctccgttcgc tatccgcccg
     2461 aaggtgagcg gtattggggt ccgttctacg ccccgctgcg ctgggacgta tccatgcgtg
     2521 agtacctgga tcgagccaat gaggaagtac ttgccatcgg aacaatcgag catattgacg
     2581 ctttggacac cgtgaactcg gtcgtcgcca ccgcaggact cgacctcgtt tttattgggc
     2641 ccggagacct ggcaacaagc atggggctca aaggcagggt ggatgatccg tccgtccaaa
     2701 gcgccattgc taagttggaa gcgcaaatcc tggcaagccc cgtgttgcta ggtggggtag
     2761 cccatacggc cgagcaggcg agcgcgatga tcgctcgggg ctataaggcg ctggttgtcg
     2821 gcttcgactg gtcatttctg caacgtggga ttgccgc
//
LOCUS       DATMGX010000437         2673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_417950, whole genome shotgun sequence.
ACCESSION   DATMGX010000437 DATMGX010000000
VERSION     DATMGX010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417950"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..640
                     /locus_tag="VNR65_07060"
     CDS             38..640
                     /locus_tag="VNR65_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HWJ18467.1"
                     /translation="MSASSDLNISVDREREVIHDHWVMFLIQGVILAALGLLAIGAPF
                     LATVVVVKLAGWLFLIGGVVGLASLFTGRGVPGSFWSFLSAIVSILAGFYILYRPLSG
                     MLSLTLVLAAFFFAQGITQIFASLSHRRVLKSWGWVLLSGIVDLILGGIIMSGWPETS
                     AWVLGILVGVNLFMFGVALIMTAFASKDVTPVAAPRAYRA"
     gene            complement(628..1128)
                     /locus_tag="VNR65_07065"
     CDS             complement(628..1128)
                     /locus_tag="VNR65_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18468.1"
                     /translation="MAARSPRDVIEYFDDTSQNEDETPFCPVRPDDSPDAWSAFWGGI
                     LIGLSGAALVTLAPWLGGSLIFVGYGMTALSLRGSRYGFLRALSFGYALLACLGAAMV
                     LGQVFFPNITASVIDAAARHHVILISLATLAWPIALLKYLFKLATSKAPPAAVRRRFR
                     AKDQAR"
     gene            complement(1446..2078)
                     /gene="ruvA"
                     /locus_tag="VNR65_07070"
     CDS             complement(1446..2078)
                     /gene="ruvA"
                     /locus_tag="VNR65_07070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWJ18469.1"
                     /translation="MIGKLRGLVDAVGADHAIIDVGGVGYEVACSSRTLSALPAPGEA
                     VTLSIETHVREDAIKLYGFLSEAERGWFRLLQSVQGVGSKVALSILSTLDVGQLANAI
                     ALQDRSMVSRAPGVGPKVALRLVNELRDKAPPLALVTTQVAGQAPSQGNGIKNASIAP
                     SPAAEAVSALVNLGYAPLQANAAISAAMSKAGEEARTEDLIRLGLKELAR"
BASE COUNT          590 a          728 c          756 g          599 t
ORIGIN      
        1 ccaatgcaac cgaagcgtaa aatccagggt gtaagcgatg agtgcaagtt ccgacttgaa
       61 catttcggtg gatcgggaac gagaggtgat tcacgatcac tgggtgatgt tcctgattca
      121 aggggttatc ctcgccgcgc ttggcctcct ggccatcggg gcaccgttcc tggcaacggt
      181 tgtcgtcgtg aaactggccg gatggctttt cctgatcggc ggtgtcgtcg gcttggcgag
      241 cctgttcacc gggagagggg ttcctggctc cttctggagc tttttgagtg cgatcgtctc
      301 aatcctcgca ggcttctata tcctttatcg cccgctttcc ggcatgttgt cccttacgct
      361 tgtgcttgcg gctttcttct ttgcgcaggg gatcacccag atcttcgcat cgctttctca
      421 caggcgcgtg ctgaagtcat ggggctgggt gctcttgagc ggcattgtcg acctcattct
      481 cggcggcatt atcatgagtg gctggcccga gacttcggcc tgggtgctgg gaatccttgt
      541 tggcgttaat cttttcatgt ttggcgtcgc gctgatcatg acagcgttcg caagcaaaga
      601 tgtaacgcct gtcgcggcgc cgagagctta tcgcgcttga tcttttgcac gaaacctccg
      661 gcgaaccgcc gccggaggtg ccttggaagt agcaagcttg aagagatatt tgagaagggc
      721 gattggccaa gctaaagtcg ccagagaaat taagatgacg tgatgtctgg ctgcagcgtc
      781 gatcacagat gctgtgatgt tcggaaagaa cacttggcca aggaccattg cggcgccaag
      841 acaggcaagt agagcgtatc cgaagctcaa agcgcggagg aagccatagc gacttccccg
      901 cagcgataac gccgtcatgc cgtatccaac aaagatcagc gagcccccaa gccagggcgc
      961 aagagtaaca agagccgcgc ccgaaagccc gatcaggatg ccgccccaaa aggcagacca
     1021 cgcatccggc gagtcgtcgg ggcggaccgg gcaaaacgga gtttcgtctt cgttttgcga
     1081 ggtatcgtcg aagtattcga tgacgtcgcg tggagaccgc gcggccatgc attcctcctc
     1141 aaaaggatcg gactcttgtc tctaaggaag acaagaacca atccggcttt ggggatgtag
     1201 tcttgagcgg gaggacttga agagattgac ctgcctgctt atgccaacaa aaaatattta
     1261 taaaacgctt gacaactctt cggcgccgca ctggatcgct atcccacacg aacgaccata
     1321 tgtcatggcc agcaaaaagt atttacatgt ttatttagaa gggccgtttt acgaaacaaa
     1381 accaatttta accgctcgta caccctcaca ggattacgcc cagaatcatg aacgcggcct
     1441 ggatgttacc gcgcaagttc tttcaagccg aggcgaatga gatcctcggt cctggcttcc
     1501 tcgccggcct tggacatggc ggccgagatc gccgcattgg cctggagcgg cgcataacca
     1561 agattgacaa gcgccgatac ggcctccgcg gccggggagg gcgcgatgct ggcgttcttg
     1621 atgccgtttc cttgtgaagg ggcttgtcca gcaacctggg tggtcaccag cgcgagggga
     1681 ggcgctttgt ctctgagttc gttcacgagg cgaagcgcaa ccttggggcc aacccctggc
     1741 gcgcgcgaga ccatgcttct gtcctggagc gcgatggcat tggcgagctg tcccacatcg
     1801 agtgtgctga gaatggacag cgcgactttc gagccgacgc cctgcacgct ttgcaggaga
     1861 cggaaccagc cgcgctcggc ttcgctcaga aagccgtaga gcttaatcgc atcttcgcgg
     1921 acatgcgtct cgatggagag tgtaacggct tcgcccggcg ctggcagtgc cgacaatgtc
     1981 cgcgaggagc aggcaacttc gtagccgacg ccgcccacat cgatgatggc gtgatccgcg
     2041 ccgaccgcgt cgaccaatcc tcgcaatttt ccgatcatgc ctgaaatcta gcccgagtcg
     2101 tgcgcccggc tgtgcgcttg cttgaatttt acacaagtcc gacggctcat ttgaatacgc
     2161 attgcgaaag gcggcatctc gaatccccgg aacagcttgt gtttgcgtcc ccattgcttg
     2221 aggcggaatc ttaggaaaat gcgctttacc gggaggtaaa tttagctaat ccattgatat
     2281 aaaacggaaa acttgaaagt gataggcgcg gctgcggcta cagaaggagc tatcaaggtt
     2341 aacggtctgg tccaaaaaca taattaacgg attttttcgc gccgcgcttt ttcgcaaaaa
     2401 acgcatcttt tggcgcgaat tcaggccgga cataattccg gcgcgccgga attaaccata
     2461 agcagttaat tcaccccgct gccgcaacgc tattttcacc atattgggcc tgaattggac
     2521 cgcaatcata caacgaccag cgtttttgca tgcattgtca gggtacgctt gacgacaaat
     2581 ggtccaaaag tctcgccgcc gccttatttc gcgcctctgc gaagcaattg tcccaagcac
     2641 agcctgtttc agtttcgcca acgaatgaag aga
//
LOCUS       DATMGX010000438         4080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_4225,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000438 DATMGX010000000
VERSION     DATMGX010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4225"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VNR65_07075"
     CDS             complement(<1..888)
                     /locus_tag="VNR65_07075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ18470.1"
                     /translation="MSARSLSVNLKPDALMRYETGPLVFQKMAPPALLATRSRPDPVR
                     VAVIGNHLPRQCGIATFTTDLCDAFAAAYGTAGLMVVAVNDPQSSYGYPARVQFEITE
                     NDLSSYKAAADFLNSSNIDIVCLEHEYGIFGGKAGGYILRLLQHLKMPLVTTLHTVLR
                     EPDVDQLIVMQEIAARSDRLIVMSEHSSRFLRNVFGVSREKIDLIPHGIPDMPLVDAA
                     SHKDSSFEGKTVLLTFGLLSSNKGFESVIRALPRILSRHKNVVYVIAGATHPHVRRRE
                     GDRYRLELQGLAESLGVKEH"
     gene            complement(1332..2024)
                     /locus_tag="VNR65_07080"
     CDS             complement(1332..2024)
                     /locus_tag="VNR65_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWJ18471.1"
                     /translation="MAGKRASSQPDWHRVFGQGSGLRKTPCRAGLYARVSTHDQQTLP
                     LQSRAMREYAARRGWSVVVQVREVGSGASERELCEQLLDAARRREIDVVLVWRLDRWG
                     RSVADLIAMLQELSHLGVGFVSLTEALDLTTPIGRAMAGLLAVFAEFEREILGERVRA
                     GLAHARQNGKRLGRPPTAARKGSQVMIKWTKGIFGDRSKPSPTPHFTALSATRRRPLA
                     APGERLPQASNL"
     gene            complement(2089..3726)
                     /locus_tag="VNR65_07085"
     CDS             complement(2089..3726)
                     /locus_tag="VNR65_07085"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWJ18472.1"
                     /translation="MQPAAPPLDVTPEELEALLEGVREALGEAGYQKLKAAIRTLSYV
                     TQLLENREATLQTLRRLLCQARTEKTEAVLQQAGIEGSPTKRRPAAAAAAKHPAAGHG
                     RNGAEAYRGARRVEVQHASLHHGDRCPECQRGKVYPLHDPGLLVRMKGQAPIEATVYA
                     LEKLRCNLCGETFTAVAPEGVGEEKYDATTASMIALLRYGSGFPWNRLERLEESLGIP
                     LPAATQCEIVAEAAAAIQPAMVELIGHAAQGEVLHNDDTSMPVLALRRDARHEDVHTE
                     DAAAERTGVFTSGIVSTAHGQYIALFFTGRQHAGENLADVLKQRAAELAPPIQMCDAL
                     SRNLPKLPEKLEVIVGHCLAHARRRFVEVTSNFPEECRHVLEQLGEVYSHDARAREQG
                     MMPDERLRLHQLHSGPVMDQLHVWLTAQFAEKKVEPNSGLGMAIRYLLKHWERLTLFL
                     RQAGAPLDNNICERALKKAILHRKNSLFYKTENGARVGDLFMSLIHTCELCGANPFDY
                     LTELQRHAAELAWNPSRWMPWNYRATLPQSGIGVDSG"
     gene            complement(3726..>4080)
                     /locus_tag="VNR65_07090"
     CDS             complement(3726..>4080)
                     /locus_tag="VNR65_07090"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruA"
                     /protein_id="HWJ18473.1"
                     /translation="WSAAARRRNLHFLAYNTRFLILPWVEVPHLASHLLGGMAKRLSE
                     DWEHLYQHPIYFLETFVDPTRFRGTCYRAANWIRLGQTTGRGKDDQTNRPNRSIKEVL
                     GYPLTRRFRELLQEA"
BASE COUNT          796 a         1256 c         1179 g          849 t
ORIGIN      
        1 gtgctctttg acaccaaggc tctcagctaa accctgcaac tcgagccggt atctatcgcc
       61 ctctcggcgt ctgacatggg gatgagtggc gccagcgatt acgtagacga cgttcttgtg
      121 ccgggacaag atgcgcggca acgctcgaat tacactttcg aaccccttgt tcgaagataa
      181 caagccaaat gtgagtaata ccgtctttcc ctcgaatgag gaatctttgt gagaagccgc
      241 atcgacaagc ggcatatcgg gaatgccatg gggaatcaga tcaatcttct cgcgtgaaac
      301 tccaaataca ttccgcaaga atcgagagga atgctcgctc atgacaataa gacgatcaga
      361 gcgcgcggcg atttcttgca tcacgatgag ttggtcgacg tcgggttcac gaagaactgt
      421 atgaagagtt gtcaccaggg gcattttcag gtgctgcagc aggcgcaaga tatacccgcc
      481 ggcctttcct ccgaaaatcc cgtactcgtg ctccaaacaa acgatgtcaa tattgctgga
      541 attcagaaag tcagcggctg ctttgtagga tgaaaggtcg ttctcagtta tctcgaattg
      601 cacgcgcgca ggatagccat atgacgattg tgggtcattc acggcgacga ccatcagccc
      661 agcagttccg taggcagccg caaaagcatc acacaaatct gttgtaaagg tggcaattcc
      721 gcactgtctg gggaggtggt ttccaataac cgctacccgc acgggatccg gccgactacg
      781 cgtggccagc agggctggtg gcgccatctt ttggaagacc aggggacctg tctcgtaccg
      841 cattagagcg tcaggtttca aatttacgga taaggaacgc gcggacattg cagcctcctg
      901 aggtcacgta atttgttgca gggaggtgga tgcagggtaa agaaggagtg gaggacgcta
      961 caccaggtct ccctgttggt cgcaccacct gccgcagaac acacacggcg tgcctagact
     1021 ccacattgca agtaggaaca tcactctact catgtgagcc ccctccagtt atcttcccgg
     1081 tcgattctgt ggcttcgata tggtgtgtgt tttcgcacac gcaggttgcc agacgaggcc
     1141 ggcgaatcac gtgaatcttg accttagcaa atcaggattg aagaggtctg agcaataatg
     1201 ctcacggttg ccgccgtcgt aaggcgcctc ggtggtttct tcccggtgcg agtgttgatg
     1261 tagtgaaggc aatcgtggac ttacggcgat tcaagactgt ctacggggaa tttcagattg
     1321 gaaatctgag gttacaaatt cgatgcttgc ggaaggcgct cacccggtgc cgctagcggg
     1381 cggcgccttg tggccgagag tgccgtgaaa tgtggagtag ggctcggctt ggagcggtcg
     1441 ccgaagatcc ccttcgtcca tttgatcatc acctggctac ccttccgcgc cgccgttggc
     1501 ggccgtccca ggcgcttccc gttctgtcgg gcatgggcca agccggcgcg aacgcgctcg
     1561 cccaggatct cacgctcgaa ctcggcgaat acggccagca gaccggccat ggctcgcccg
     1621 atgggggtcg ttagatccag cgcttcggtc agcgatacga acccgacccc gagatggctg
     1681 agttcctgga gcatcgcgat caggtcggcc accgatcgtc cccaccggtc caggcgccac
     1741 accagtacca cgtcgatttc gcgccggcgc gccgcgtcca gcaactgctc gcaaagctca
     1801 cgctcggaag ctccggaacc gacctcccta acctgcacga caacgctcca gcctcgtctt
     1861 gccgcgtact cgcgcatggc acgactttgc aagggtagcg tttgctggtc gtgggtggag
     1921 acgcgagcgt acaggccggc gcggcacgga gtttttctca ggccagaccc ctggccaaaa
     1981 accctgtgcc aatcaggttg gctagaggcc cgttttccgg ccatcttcga gtcgctcctg
     2041 tgtatggccg gaatcgattg tttccggcca ggcgggattc atcatactct acccggaatc
     2101 cacaccgatg ccagactgtg gaagcgtcgc gcggtagttc caaggcatcc atcgtgaggg
     2161 attccacgcc aactcggcgg catgccgctg tagctcggtg aggtaatcga acgggttggc
     2221 gccacacagt tcgcaggtat gaatcaggct catgaacagg tcgccgacac gggccccatt
     2281 ctcggtcttg tagaacagag agttcttgcg gtgcagaatc gctttcttca atgctctttc
     2341 gcaaatgttg ttgtccagcg gggctccggc ctgccgcaaa aacagcgtga gccgctccca
     2401 gtgcttgagc agataccgga tcgccattcc cagaccggag ttcggctcca ctttcttttc
     2461 cgcaaactgg gcggtgagcc aaacgtgcag ttggtccatc accgggccgc tatgaagctg
     2521 gtggaggcgc aaccgctcat ccggcatcat gccttgctcc cgcgcccggg catcgtggct
     2581 gtagacctcg cccagttgtt ccagtacatg ccggcattcc tcgggaaagt tggacgtcac
     2641 ctccacgaag cggcggcgag cgtgagccag acaatggccc acgatgactt ccagtttctc
     2701 cggcagtttg ggtaggttgc gggacagcgc atcacacatc tggatgggag gagcgagttc
     2761 cgccgcccgc tgtttgagca catcggccag gttctccccc gcgtgctggc gtccggtgaa
     2821 gaacaatgcg atatattgcc cgtgcgcggt cgatacgatc ccgctggtga acactcccgt
     2881 gcgctccgcc gcagcatctt ccgtatggac atcctcatgg cgcgcatccc ggcgcagcgc
     2941 cagcacgggc atggaggtgt cgtcgttgtg cagtacttcg ccttgcgccg cgtggccaat
     3001 cagctccacc atcgcaggtt ggatcgcggc ggccgcttcc gctacgatct cgcattgcgt
     3061 cgcggccggc agcggaatgc ccagactctc ctccagcctt tccaatcgat tccacggaaa
     3121 cccactcccg taacgaagca gcgcgatcat gctcgccgtc gtcgcgtcgt acttctcctc
     3181 ccccacgcct tcgggagcca ccgccgtgaa cgtttccccg cacagattgc agcgtagctt
     3241 ttccaacgca tacaccgtgg cttcgatcgg cgcttggcct ttcatccgca ccaatagtcc
     3301 aggatcatgc agcgggtaca ccttgccgcg ctgacactcc gggcaccgat ccccgtggtg
     3361 caaggacgca tgctgcacct cgacccggcg tgctccacga taagcttccg ctccgttgcg
     3421 cccgtgtccg gcggccggat gcttcgcagc cgctgccgcc gccggtctcc gtttcgtcgg
     3481 actaccctcg atgcccgcct gctgcagcac cgcctcggtc ttctccgtgc gcgcctgaca
     3541 caacaggcgg cgcagggtct gtaaagtcgc ctcgcggttc tccagcaact gggtcacgta
     3601 gctcagcgtg cggattgcgg ctttcagttt ctgatagccc gcctcaccca gcgcttcccg
     3661 cacgccctcc agcaacgcct ccaactcttc gggggtgacg tcgaggggcg gtgctgccgg
     3721 ttgcatcagg cctcctggag taactcacga aatcgtcgcg tcagtggata gcccagtacc
     3781 tctttgatcg atcggttggg acggttcgtc tgatcatcct tgccgcggcc ggtcgtctgc
     3841 cccaaccgaa tccagttggc cgcgcggtaa catgtcccgc gaaagcgcgt gggatccacg
     3901 aaggtttcca gaaaatagat cggatgttgg taaaggtgtt cccaatcctc cgacagtcgc
     3961 ttcgccatgc ccccgagaag gtgcgaagcc aggtgcggta cctcgaccca gggcagaatc
     4021 agaaagcggg tgttgtaggc caggaagtga aggttgcgcc gccgcgctgc cgcactccag
//
LOCUS       DATMGX010000439         2379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_432011, whole genome shotgun sequence.
ACCESSION   DATMGX010000439 DATMGX010000000
VERSION     DATMGX010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432011"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1020..1154
                     /locus_tag="VNR65_07095"
     CDS             1020..1154
                     /locus_tag="VNR65_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HWJ18474.1"
                     /translation="MNRAVCEPARSTFKENAMDTNTLLLIVIVLLLLGGGYYGRKRWF
                     "
     gene            1229..1609
                     /locus_tag="VNR65_07100"
     CDS             1229..1609
                     /locus_tag="VNR65_07100"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWJ18475.1"
                     /translation="MAQTLQTEIPQEMREFAVHNVDKARAACAQLMDATRKAQDMMKT
                     MIPSTPVAQGLSEVQERAMRFTQQNLDAGFSLAHELAKAGDLTEVLQIQSRHAQLQMH
                     TYALQAQELGNLVNAASLKGKPGS"
     gene            complement(2078..>2379)
                     /locus_tag="VNR65_07105"
     CDS             complement(2078..>2379)
                     /locus_tag="VNR65_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18476.1"
                     /translation="RVSAAKRLIEFIAATPGAPAAYLSAKTVSDRSIDLLPTSAGVMV
                     QPRQERGVREFTRFRFLSWPVVIAVILAILLIVISLRPTVQANEPHVTSSSNVLL"
BASE COUNT          608 a          623 c          634 g          514 t
ORIGIN      
        1 tgagcaggcc gtcgagcgca ccgataggcg ctttttgaac gcttcgttgg acggcgcaaa
       61 aaccgtaaac gatcctgccc tcctcaaggt ctccaaagtc cggcagcttt tagtgccgcc
      121 aggaacttgg aggatgatgc atcactttcg attgtgtcca ccatgtcgcg agaatttacg
      181 gcaaccaaat ttaggacggc actcacgttt aaagtccctt ccggtactaa aacaaataca
      241 ttccgtagtg cgtctggttt cttgtcagaa cagggcgtgt tctaatttta tccttaagag
      301 gatattggga tagcgtctcg attgggcaag cgcctatagg aaaactattt ttggatcaat
      361 tcaattgtca aagtaaaaaa gtcacctttt gggttgcata attacaagta gtgggaatta
      421 aggccgatcc gggtggaaaa ttctggtcaa aacagagcaa cagcatcctt tcgagccata
      481 gggccgtgat cgccggaagg gacacgggaa tcttacagac catcgctagg gcgagaacct
      541 tgagcggaac gctgtggacc tcctcatgac tgccgaccct agcctcagat ttttcatgat
      601 gagcggacga gcggtagttc agaataatgg tcgccgaatg acgatgagga acatagaaaa
      661 ctgctaaccc tccgaagcag tatctcaact agtttcccgc gatacctgtt acggaatcag
      721 aataggaaag cctgcggagc tttgcacgat cgccgttcgc aaggcagatt cgttgaactc
      781 agcttgcaaa gcgacgtaaa gcgtcggctc tcgacggtac agggcccaaa ccgccgaccc
      841 gttacgttcg acttctttat aatattaaat gcctaacatc tatagataca gaaggatatc
      901 agtctcaaga atctatgagt actttccgaa actttcaatg cggtattgcg atagccatct
      961 tgacataatt gggcatgact tcatgcccga caagctgcat cgcgtgctag cgctccaaga
     1021 tgaaccgcgc tgtctgcgag ccggctcgtt caacattcaa ggagaatgct atggatacca
     1081 acaccctctt gcttatcgtc attgtgctcc tcttgctcgg cggcggctat tacggtcgca
     1141 agcgctggtt ctaactgcag ctgagccggc gtgcctagcg aggcagccgg cgtgatgaat
     1201 gcaaaacagc agcacaggga ggagacccat ggcacagact ctgcagacgg aaatacctca
     1261 agagatgcgc gagttcgccg tgcataacgt cgataaagct cgcgcggctt gcgcccaact
     1321 gatggacgcc acccgcaaag cgcaggacat gatgaaaacg atgatcccct caacccccgt
     1381 ggcgcaggga ttaagcgaag tgcaggagcg ggcgatgagg tttacccagc aaaacctcga
     1441 cgccggtttc tcattggccc atgagcttgc caaggcaggg gacctgacgg aagtgctaca
     1501 aatccagagc aggcatgcgc aactgcaaat gcacacctat gcccttcagg cgcaggaact
     1561 tggcaacttg gtaaacgcgg cttcgctgaa gggaaagccg ggcagctgag tggcggagcc
     1621 tactccgccg attttttatg ttccgctgcg aaactataag tcgggcaacg cggctgggcg
     1681 cggtgcttcg cgttccgccc cagccgcgtt ttccggctta accaaagcca aaaacggcca
     1741 caaaactgac atcgggcgat ggaaagactg tcgcgtctgc cagccgtcta gatagaggcg
     1801 tatcaggctt gatcgctatg cgatagcttc ggaatctgcc tatgaggcta ataagcaaca
     1861 cagcgcatca aagcgcgacg cgagcctaac tgcccgccgt tcgacgcagc agccttatgt
     1921 aaagaacgaa ccgtcataaa tcatgacgcc tttgcccacg gcagtccctg gtcgcgcgct
     1981 aaaattaatt acatgcttgt ttgtggtcat ttttgcgaaa cgaaccaatt tagttaaccg
     2041 aaattcacac aggaatcacg cctggaaaag ccccgaatta gagcaggacg ttgctggagg
     2101 atgttacgtg cggttcattc gcctgcacgg ttggtcgaag gcttattacg atcaatagga
     2161 tcgccagaat gaccgcaatt acgaccggcc aactcaggaa gcggaacctt gtaaattccc
     2221 gaacgccacg ttcttgccgg ggctggacca tgacgccagc cgaggtcggc aaaagatcaa
     2281 tcgaccgatc ggagaccgtc ttggcgctga gataggctgc tggggcgccg ggtgtcgctg
     2341 caatgaactc gatcagtctt ttggctgcgg agacgcgtt
//
LOCUS       DATMGX010000440         2688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_346728, whole genome shotgun sequence.
ACCESSION   DATMGX010000440 DATMGX010000000
VERSION     DATMGX010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346728"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2300
                     /locus_tag="VNR65_07110"
     CDS             <1..2300
                     /locus_tag="VNR65_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWJ18477.1"
                     /translation="SESELKETLGQLTKSRSLLEPLAADPSLRGVMGAITLTLKGVQA
                     KRITLDSLAPKFNAFSTAIENSINNRPTWFSWREALSGQPSSRRETRQFIEIEPILNY
                     NSLEPGQDASNAIRRTALDLGLAQDGVTVRLTGPVPIADEEFGTLKDGAGLNTALTIL
                     SVLIILWLALRSARIIFAVFVSLFIGLAITAALGLAMVGAFNPISLAFFVLFVGIGVD
                     FGLQFSVGYRAERFERNQLIPALVANADHTGGRLMLAAFATAAGFLAFTPTAYKGLSE
                     LGLIAGVGMLIAFLTSVTVLPALLRLLKPPAEPHQLGYAFLAPVDRFLERHRVGVLIG
                     TLCVVIAGLPLLHWLRFDFNPMNLRSPNVESVATYLDLKKDPETAGRTIEVLTPSLAD
                     ADALATRLSALPVVLRATTLSSFVPQGQDKKMVLISQAAAELNETFTPPKEKVPPAPT
                     DQDIVTSLEGASNYLSVVVKRNLGTKGVQAAERLSNVLSDLAKASPEARARASDALVR
                     PLKMTLDDIRLGLNPEIVTLDSLPASLVASWKSADGRVRVTVSPKGDSNDNAVLRHFV
                     EAVTAIAPEGTGEAIGIQRAGDTIVNAFIQAAIWALLSIAILLWITLKRASDVALTLL
                     PLLLACVVTLEVCVIIDMPLNFANIIALPLLLGVGVAFKIYYIMAWREGRSGLLASPL
                     TRAVFFSGMTTAVAFGSLWLSNHPGTSSMGKLLALSLVSTMAAAVLFQPLLMGPPREV
                     EKPAEKERKGKAGSPAYLPTPEHTA"
     gene            complement(2270..>2688)
                     /locus_tag="VNR65_07115"
     CDS             complement(2270..>2688)
                     /locus_tag="VNR65_07115"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HWJ18478.1"
                     /translation="CSIGAASAQSPVGEWMVKDQTAHIRIVSCGNALWGVINWTKGPP
                     GKDENNPDPAKRTRSVMGMPILINMKPGEEQWEGEVYNAQNGQTYTSYISLKSPDVLQ
                     IQGCVLGFLCGGEDWTRVPLTKGAPSDQAVCSGVGK"
BASE COUNT          504 a          859 c          765 g          560 t
ORIGIN      
        1 tgtccgagag cgagttgaag gaaactctcg ggcaactgac aaagtcccgt tccctgcttg
       61 agccactcgc ggccgacccg agcctgcgtg gcgtcatggg ggccataacg ctgacgctga
      121 agggtgtaca agccaagcgc atcacgctcg acagcctggc gcccaagttc aacgcgttct
      181 cgaccgccat cgagaactcg atcaacaacc gccccacctg gttcagctgg cgggaggctc
      241 ttagcggaca gccttcttcg cggcgagaga cgcgccagtt catcgagata gagccgatcc
      301 tcaactacaa ctcccttgag ccggggcagg atgcatccaa tgccattcga aggaccgcgt
      361 tggatctcgg ccttgcgcag gatggcgtca ccgtacgttt gacgggtccg gttcccatcg
      421 ccgatgagga attcggaacg ctgaaggacg gggcggggct gaatacggcg ctgaccatcc
      481 tgtcggttct gatcattctc tggcttgcgc tccgttccgc gcggatcatc ttcgccgtct
      541 ttgtctccct cttcatcggg cttgctataa ccgccgcgct gggcctcgcc atggtcgggg
      601 cctttaatcc tatttcgctt gcgttcttcg tgctgttcgt cgggatcggc gttgatttcg
      661 gccttcaatt cagcgtcggc taccgggcgg agcgcttcga gcgcaaccaa ttgatccctg
      721 ctcttgtcgc aaatgccgac cacacaggcg gccgccttat gctggcagca tttgcaaccg
      781 ccgccgggtt cctggcgttc acgccgacag cttataaagg cctttcggaa ctcggtttaa
      841 tcgcaggcgt gggcatgctc atcgccttcc tgaccagcgt cacggtgctc cccgcactcc
      901 tgcgccttct caagccgcca gccgagcccc atcagctcgg ctatgccttc ctggccccgg
      961 tcgaccgctt cctcgaacgg catcgcgtgg gggtgcttat cggcacgctc tgcgtcgtta
     1021 tcgctggcct gcccctcctt cattggctgc gtttcgactt caatcccatg aacctgcgca
     1081 gcccgaatgt ggagtctgtc gccacttatc ttgacttgaa aaaggacccc gaaacggctg
     1141 ggcgcacaat cgaggtgctg acgccatcat tggcggatgc agacgccctc gccaccaggc
     1201 tcagcgcgct gccggtggtg ctgagagcca cgaccttgag cagcttcgtg ccgcagggcc
     1261 aggacaagaa gatggtcctc ataagccagg ccgctgccga actgaacgag acgttcaccc
     1321 ccccgaaaga aaaagtgccg cctgctccaa ccgatcagga catcgtcacg agcctcgagg
     1381 gcgcatccaa ttacttgagc gttgtcgtta agcgtaacct gggaacaaag ggcgtgcagg
     1441 ccgcggaacg gctgtcaaac gtgctttcgg acttggcgaa ggcaagccct gaagcacggg
     1501 cgcgcgcctc cgatgcgctc gtaagaccgc tcaaaatgac gttggatgac atccggctcg
     1561 gtctcaaccc cgagatagtc acgctcgact ccctgccagc aagtcttgtt gcaagctgga
     1621 aatcggcaga cggccgcgta cgggttacgg tgtccccgaa aggagattcg aacgacaatg
     1681 cggttttgcg ccatttcgtc gaggcagtaa cggcgatagc gcccgaagga acgggagagg
     1741 ccatcggcat tcaaagggcg ggcgatacga tcgtcaacgc ctttatccag gccgcgatct
     1801 gggcgcttct ctcgatcgct attctgctct ggatcacgtt aaagcgcgcg tccgacgttg
     1861 cgctcaccct cctcccgctt ctcctcgcct gcgtcgtcac actcgaggtg tgcgtgatca
     1921 tcgacatgcc gctgaatttc gcgaacatca ttgcgctccc gcttctctta ggcgtcggcg
     1981 tcgcgttcaa gatttactac atcatggcat ggcgtgaggg gcgaagcgga cttctcgctt
     2041 ccccgctcac acgcgccgtg ttcttcagcg gaatgacgac cgccgtggcc ttcggcagct
     2101 tatggctctc gaaccaccct ggaacatcga gcatgggcaa actgctcgcc ctttcgctcg
     2161 taagcaccat ggcggccgcg gtgttgttcc agccccttct gatggggccg ccccgcgagg
     2221 tggaaaagcc agctgagaaa gagaggaaag gcaaagccgg aagccccgcc tacttgccca
     2281 cgcccgagca taccgcctga tcagaaggtg cacccttggt gagcgggacg cgggtccaat
     2341 cctcgccccc gcaaagaaac cccagaacgc atccttggat ctgaagcacg tcggggctct
     2401 tgaggctgat ataggacgta taggtctggc cgttttgagc gttatagacc tcgccctccc
     2461 attgctcctc cccgggtttc atattgatga gaatcggcat tcccatcacg gagcgggtac
     2521 gcttggccgg atccggattg ttttcatcct ttccgggagg acctttcgtc caattgatta
     2581 cgccccacag cgcatttccg catgacacga tgcggatatg ggcggtctgg tccttgacca
     2641 tccattctcc cactggggat tgagcagacg ccgccccgat cgagcata
//
LOCUS       DATMGX010000441         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_254581, whole genome shotgun sequence.
ACCESSION   DATMGX010000441 DATMGX010000000
VERSION     DATMGX010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254581"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1110..1484
                     /locus_tag="VNR65_07120"
     CDS             1110..1484
                     /locus_tag="VNR65_07120"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWJ18479.1"
                     /translation="MADNPFEIPQPMRDVAEQNIKQAHAAYEQLTDFVTKAIDTWTGA
                     MPASPMATGFKDIQHRAAEIAKTNADSAFMLVEKITKAQNFQEILTLQTQFAQDQMKA
                     FTTQTQELHRLIGETVQKVQHS"
     gene            1605..1922
                     /locus_tag="VNR65_07125"
     CDS             1605..1922
                     /locus_tag="VNR65_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWJ18480.1"
                     /translation="MIEITGTKNDRLLQLALELERIALQDDYFIEEKLYPNVDLYSGI
                     PLKALGFPPSMFTVLFALGRTVGWIAQWKEMIADLEQKIGRPRQLYIGEKARHYVPLE
                     LRP"
BASE COUNT          503 a          574 c          584 g          400 t
ORIGIN      
        1 aagctacggt aacggctgcg ccgaaggcgc ctcaacagga atgtatggat tttccctcat
       61 gggtcgcaaa gagacgtgca agatatttga acaaaattcc gcttgtgccc cggcacctgc
      121 cgcattcttt cggcccgccg ggcgtggttt gatgggcgag cttgacccaa accggccctc
      181 gctaccagat gaagactgcc gcccctcctc agccgggcaa agatgactgt gattgcggtg
      241 cgtgccgagg agatgctttc ggaccagccg gaactcgccg ctgctgtcga gccgctggac
      301 gatcgctatc tgcatcgtga atgataagga caagatcatg cgcgaggggt tggttacttc
      361 cgatccggag cgaatgaatc agaacccctg ggtgaccccg ctttaggggt ttcgttggtt
      421 gtgagcgtca aaccagcgtc ctcggccctg gaagcgcctt atcacgccgc ctgttcacgc
      481 cacttccgca taaactcccg catgatcgca cggtattgct ccaggtgagc ggctcgccag
      541 cgcgctctct gatgcaggcg cgacctcttg acgctaagca gctaggggaa cgaaggtgag
      601 cttagagagt tagaaccgca ctacaacctt aatcgggcga agcaaaacgg ggggcgtctt
      661 ccaaaagggg gcgccctttg tatttggccc gcgaggcgcg ccgctggggg gtggcgatga
      721 acgccaagcc agcgtcctcg cccttataag ccgtagggat catttgaggg ttcctccggg
      781 cgcgcgtgat gttcgcgggc gacgcgcaac cctacaacga gctaggcttg gccgccgttc
      841 ggcgccatca gctatcagtc gctcgatggc ttgcgcctca tcctacaggc gcccttcgat
      901 atccttcagc cgcgccgccg aagctgttca gacaaaaaga agccgcgtct ccctaaagac
      961 gaggcaaagt tccgacaagc gagagcgtag cttgtactat acaaaaagca gatggttttg
     1021 atttctgtca tagaagcact tgcgcggggg cgagtatttg tatggttaca cttacttctc
     1081 acaaaccagc ccccgacgag gaggacttta tggccgataa cccatttgaa atcccgcagc
     1141 caatgcgcga tgtggccgaa caaaacataa aacaggcgca tgccgcgtat gagcagctca
     1201 cagatttcgt aaccaaagcc atcgacacct ggacgggtgc gatgcccgca agtccaatgg
     1261 ccacaggctt caaggacatc caacaccgcg ctgcggaaat cgcgaagacg aatgccgact
     1321 cagcgttcat gctggtcgaa aagatcacca aggcacagaa ttttcaggaa attttgacgc
     1381 ttcagacgca gttcgctcaa gaccagatga aggccttcac cacgcaaacg caggagcttc
     1441 acaggctgat cggggaaact gtccagaagg tgcaacacag ctgatttaca aaaaaagccg
     1501 cgcctcgcta aagaagccca acacctaacg gggtttcact tcaatcaggc acattgtcga
     1561 ccggcgtgac gttcgctgcc agaaaaggaa atggctatga gtaggtgatc gagatcactg
     1621 gcaccaagaa cgatcggctt ttgcagctcg cgctggaact cgagcggata gcgcttcaag
     1681 acgactattt catcgaggag aagctttatc cgaacgtcga cctttattcg ggcatcccgc
     1741 tgaaggcact tggctttcct ccctcgatgt tcacggtgct gttcgccttg gggcgcacgg
     1801 ttgggtggat tgcgcaatgg aaagaaatga tcgccgatct ggagcaaaag atcggccggc
     1861 cgcgccaact ctacatcggc gagaaggcgc gccactacgt gccgctagaa ttgcgccctt
     1921 aaggcatgtc caaccgcaag acggaacatg atcaacaggg aggaacacag gaatgaatac
     1981 cgtacagaaa gacggacgcg attcagaagt cgtgaaaata gatcttgaga cagtgaaaag
     2041 gctcgaggca gtgcttggcg a
//
LOCUS       DATMGX010000442         5575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_127015, whole genome shotgun sequence.
ACCESSION   DATMGX010000442 DATMGX010000000
VERSION     DATMGX010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127015"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..641
                     /locus_tag="VNR65_07130"
     CDS             15..641
                     /locus_tag="VNR65_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWJ18481.1"
                     /translation="MNDIEAICRLQDGRRLVSVLSAERMFRTEDLRKAVSDADKDNEM
                     SSTEKGSSVADNEQEEQFVVFRLMEEEYGVPIEAVQEIVRVPDELTRIPKTPDFIEGV
                     VNLRGVVLRVLDQRSRFGLPGAERNDRQRIMVFTINGVRTGFVVDSVSEVMRIALRHV
                     GPAPALSARQQRLINRVANLEDQKRMILLLEVEQLLNFNELDELKLVA"
     gene            702..1817
                     /gene="cheB"
                     /locus_tag="VNR65_07135"
     CDS             702..1817
                     /gene="cheB"
                     /locus_tag="VNR65_07135"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HWJ18482.1"
                     /translation="MIKVLVVDDSALMRKHLVTLLESEGGFQVRVARNGVEALSLLSS
                     FDPDVITLDINMPEMDGITCLSHIMVNKPKPVVMVSSLTEKGAEATLQALSLGAVDYV
                     HKPDGTISLSIERVHRELLLKVKGAARARVRTALGLSDALQKARLSNREHRQERPAGL
                     SPISNAADMGLVLIGVSTGGPGTLEEILPLLPVNFPWAVLVAQHMPGSFTNVFAKRMD
                     SVCAMPVQEVSRQSPIEPGKIYVAKGDADLVVMGRGKSLLAVPVPSSQDHLWHPSVTR
                     LVTSAMESVAVDKLIGVLLTGMGDDGAAAMAELRRRGGRTIAQDEATSIVFGMPSELI
                     RLGGAAVVLPSHAIARAITTWLYPSTLTNRSRPYGAR"
     gene            1804..2436
                     /locus_tag="VNR65_07140"
     CDS             1804..2436
                     /locus_tag="VNR65_07140"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWJ18483.1"
                     /translation="MALVRASNRPASAQAGAEQLSRPSLADLAVQLRADEPAQRRQAA
                     RDLALTSEGIPILCEHLEHEPAVSVRSVIMTSLIQSRSRDVVEGLARHLRSENTALRN
                     EAIEALQDMPEQIAPYMEQLLSDPESDVRIFAVNILSVLPDAHAPDWLRKVILEDPHV
                     NVCAAAVDCLAEIGTPDSIAALKELRLRFGDHPFMIFAIDAAIRRIGESQ"
     gene            2433..3299
                     /locus_tag="VNR65_07145"
     CDS             2433..3299
                     /locus_tag="VNR65_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HWJ18484.1"
                     /translation="MKPASPPAFLHQEAPSVTQQEFAKFCEFFYRKTGIIFTENKRYF
                     VERRILERMVASQCPTFREYFATVRFETAGQEMQRLVNAMTVNETYFFREDYQFDTLV
                     SGILPELAVNKKPGDTIRIWSLPCSTGEEPYSIAIYILEGWDRADDFNIEIIASDIDS
                     QVLSQAQEGIYGERALSRIPPDIRQKYFSSVGGGSFQILAELRESIDFFAVNVVDDAQ
                     MRQNSNIDIIFCRNLLIYFDDLARRQAMETMYECLAPGGFICLGHSESMSRMSSLYKP
                     RRIGDSIVYQKP"
     gene            3318..3719
                     /locus_tag="VNR65_07150"
     CDS             3318..3719
                     /locus_tag="VNR65_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ18485.1"
                     /translation="MNTEEASTQQKKKVLIVDDGITMRMYYRDVLEKAGFIVEEAANG
                     VEGLEKMLAGGFDLAVVDINMPKMNGYEFVRHLREDPASQSIPVMTVSTEDQERDKVK
                     AYEAGANFYMVKPIRPDELEQVARLFTGRAQ"
     gene            3716..>5575
                     /locus_tag="VNR65_07155"
     CDS             3716..>5575
                     /locus_tag="VNR65_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWJ18486.1"
                     /translation="MNPLLARFIPEAQELIQQSAAGILRLEKDPNNDVAINEVFRAVH
                     TLKGSSGLFDAQPLTRLVHAGEDVLSAVRSGALQLNSEIADHILDMLDQAGIWLGDME
                     KDETLPPAAGEVAHKLANVLRSFLPQSADAVAGPEAQAANQTGRIDWLDAMRRQPEFA
                     AFAAGAAASSRTVTAIEYHPEEACFYSGEDPVNLMRQVPELGVLSISLRTPWSSLDDL
                     DPYRCNLFFRALSVAPRDEVAHLFRYVLDQVSILSLPASVLFGDGKAEQSPGSSVAAA
                     GSPVQDVALHIAREQLRILALPGDESEQEKRLASVISILPNILAALGDDKLTQNFAAA
                     CKDNTPASMVAFIKCWVMGSKAEDVHPQKNGRVPKSLGAVSKAPAPTFSEEPVPESTR
                     FNGAAHKPQALKVDQAKLDTLMNLIGELVVSKNSLPFLARRAEEVYGSREMSREIKEQ
                     YGVIDRLAQEMQTAIMTVRMQPVSEAFDRFPRLVRDLARKLSKKIDLRMEGEETAADK
                     AIIAALGEPLLHIVRNSIDHGVETPEERIAVGKSSQAEILLRAYQESDQIVIEVIDDG
                     RGIDPAKIKAAALSKGVITEDQAARLSDQESINLVFQPGFSTTSEVSDLSGRGV"
BASE COUNT         1223 a         1588 c         1609 g         1155 t
ORIGIN      
        1 gccaaggaca ggccatgaac gacatcgagg cgatttgtcg gcttcaggat ggccggcgtc
       61 tcgtttcggt tctttcggcg gaacgcatgt tcaggaccga agacttgcgc aaggcggtaa
      121 gcgatgcgga caaggataat gagatgtcat ctacggaaaa aggcagttcc gtcgctgata
      181 acgaacaaga agagcagttc gtcgtgttcc ggctgatgga ggaagagtac ggggttccta
      241 tagaggccgt gcaagaaatt gttcgcgtac cggatgaact gacgcgcatt ccgaaaacac
      301 ctgatttcat cgaaggggtg gtcaatctgc gcggagttgt gctgcgcgtt ctggatcaac
      361 gaagccgttt cggtcttcct ggcgccgagc gcaacgatcg ccagcgcatc atggtgttca
      421 ccatcaatgg ggtgcgcact ggttttgttg tcgattccgt ttcggaagtg atgcgtatcg
      481 ccctgcgtca tgtcggcccc gcgccggctc tgtcggcacg gcagcagcgt ctcattaatc
      541 gcgttgcgaa cctcgaagat cagaagcgga tgatcctgct cttggaggtc gaacagctgt
      601 tgaatttcaa tgagttggat gagctgaaat tagtggcgta aatttttgcg ggagaggaat
      661 gcctctcccg tttgatccat ttccaggtga gacgaaagcc aatgatcaag gtgctggtcg
      721 ttgatgattc cgcactcatg cggaaacatt tggtcacgct gttagaaagc gaaggagggt
      781 tccaggttcg cgtcgcgcgg aacggcgtgg aagccctttc tctccttagc agtttcgatc
      841 ccgatgttat caccctcgat atcaacatgc ccgagatgga cggcatcacc tgcctgtccc
      901 acatcatggt caacaagccg aagcccgtcg tcatggtttc gtcgctgacg gaaaagggag
      961 cggaggccac cttgcaagcg ctgagcttgg gcgccgtcga ttatgttcac aagccagatg
     1021 gcaccatttc attgtccatc gagcgcgttc atcgcgaact gcttctgaaa gtcaagggcg
     1081 cagcaagagc gcgggtgcga actgccctcg gactgagcga cgccctccag aaggcccggc
     1141 tttcgaacag ggaacaccgg caggagcgtc ctgccggctt gtccccaatt tcgaatgcgg
     1201 cagacatggg gcttgtgctc atcggcgtat cgacgggagg accggggacc ttggaggaaa
     1261 tccttccgtt gttgccggtc aattttccct gggcggtttt ggtcgcgcag cacatgcccg
     1321 gcagcttcac caatgttttc gccaagcgaa tggacagcgt ctgcgcgatg cccgttcaag
     1381 aagtttcgcg tcaatctcca atcgaacctg gaaaaatcta cgttgcgaag ggtgacgccg
     1441 atctggtcgt aatgggccgc ggcaagtcct tgcttgcagt cccggttcct tcgagccagg
     1501 accatttgtg gcacccgagc gtgacccgtc tggtcaccag cgccatggaa tcagtagccg
     1561 tcgacaagct tatcggggtt ctcctgactg gcatgggcga tgacggggcg gcagcaatgg
     1621 ccgaactgcg acgccggggc ggccgcacga tcgcacagga cgaagccacc tcgatcgtgt
     1681 ttggcatgcc ctccgagttg atcaggctcg gtggcgccgc agttgtcctc ccatcgcatg
     1741 ccatcgcccg cgcgattacg acctggctgt acccatccac cctcacaaac cggagccgcc
     1801 cctatggcgc tcgttagagc ctcgaacaga cccgcaagcg cgcaagccgg ggcagaacag
     1861 ctatcgcgac cgtctctggc ggaccttgcc gtgcagttgc gagccgacga gccggcgcaa
     1921 cggcggcagg ccgcgcgaga cctggctctg acaagcgagg gcatcccgat cctgtgcgag
     1981 catctggagc acgagccagc ggtcagcgtg cgcagcgtga tcatgacatc gcttattcaa
     2041 agccgctcgc gcgacgttgt cgagggcttg gcccgccatc tgcgcagcga aaacaccgcc
     2101 ttgcggaacg aggcgatcga agcgttgcag gatatgcccg agcagatcgc cccttacatg
     2161 gagcagcttt taagcgaccc tgagagcgac gttcgcattt tcgcggtcaa tattctctcc
     2221 gtactaccgg acgcgcacgc gcccgattgg ctccgcaagg ttatcctcga agatccgcat
     2281 gtgaacgtct gcgccgccgc cgtcgattgc ctggccgaga tcggaacacc ggactcgata
     2341 gctgcgctca aggaactgcg gttacgattc ggcgatcatc ccttcatgat ctttgctatc
     2401 gatgccgcga tcaggcgcat aggcgagtca caatgaaacc cgcaagccca cctgcgtttt
     2461 tgcatcagga agcaccttct gtaacccagc aggagttcgc aaagttttgc gaattcttct
     2521 accggaagac cggtatcata ttcacggaaa acaaaaggta cttcgtagag agacgtatcc
     2581 tggaaaggat ggtggcgtcg cagtgtccta ccttccgtga atactttgcc acggttcgtt
     2641 ttgagaccgc agggcaggag atgcagcgcc tcgtcaacgc gatgaccgtt aacgagacct
     2701 atttctttcg ggaagattac cagttcgata cgctggtaag cggcatcttg ccagagctag
     2761 ccgtgaacaa gaaacccggg gacacgattc gtatctggtc cctgccttgc tcgacgggcg
     2821 aggagcctta ttccatcgca atatatatat tggaaggatg ggacagggct gatgatttca
     2881 atatcgaaat catcgcttcc gatatcgact cccaggtgtt gagccaggcg caggaaggca
     2941 tctatggcga gcgcgctttg tcgcgaatac cgcctgatat ccggcagaag tacttttcgt
     3001 ccgttggggg tggctccttt cagatcttgg cggagctgcg cgaatctatc gacttctttg
     3061 cagtcaacgt ggtcgatgat gcgcagatgc gtcaaaactc aaacatcgac atcatctttt
     3121 gcagaaatct cctgatttac ttcgacgact tggcccggcg tcaagccatg gaaaccatgt
     3181 acgaatgcct ggcccccggc ggtttcatat gcctcggaca ctccgagagc atgagccgca
     3241 tgtcgtcact ctacaagccc cgccggatcg gcgactcaat cgtctatcag aaaccttagg
     3301 atactaaggc gatgcttatg aatactgaag aagcctccac ccagcagaag aaaaaggtgc
     3361 tcatcgtcga cgatgggatc accatgcgga tgtattaccg cgacgtgctc gagaaggccg
     3421 gatttattgt cgaggaagcc gccaacggcg tcgaaggcct tgagaagatg ctcgccggcg
     3481 ggttcgacct ggccgtcgtc gatatcaaca tgcccaagat gaacggatac gagttcgtca
     3541 gacatctgcg cgaagacccg gcctctcagt ccatcccggt catgaccgtc agcaccgagg
     3601 atcaagaacg ggataaggtc aaggcttacg aagctggcgc gaatttttac atggtcaagc
     3661 cgattcgccc ggacgaactc gaacaagtcg caaggctgtt cacagggcga gcgcaatgaa
     3721 tccgctgctt gcgcggttta ttccggaggc tcaggagctg attcaacaat cagcggccgg
     3781 tattctgagg ctcgagaaag acccgaataa tgacgtcgcg atcaacgaag tcttccgcgc
     3841 cgtccatacg ctgaagggct cttcaggcct tttcgacgcg caacccctga caagactggt
     3901 gcacgctggc gaagatgtgc tttcggctgt gcgctcgggc gcgcttcagc taaactcgga
     3961 gatcgccgat catattctcg atatgctcga tcaggcgggc atctggctcg gcgacatgga
     4021 gaaggatgaa acactccccc cggccgcagg agaagtagcc cacaaactgg cgaatgtttt
     4081 gcggagcttc ctgcctcagt cagcggatgc agttgcggga ccggaagcgc aagcagcaaa
     4141 tcagactggt cgcatcgatt ggctggatgc gatgcggcgc cagcctgaat tcgcagcttt
     4201 tgcggcgggc gccgctgctt cctcaagaac cgttaccgct atcgaatatc acccggagga
     4261 agcatgcttt tatagcggcg aagacccagt caatttgatg cgtcaagtcc ctgaactcgg
     4321 cgtcctctcg atctcactgc ggacgccctg gtcatcgctc gacgatctcg acccctatcg
     4381 ctgtaacctc tttttccgtg cactgagcgt cgcccctcgc gatgaggttg ctcacctgtt
     4441 tcgctatgtc ctggatcaag tctcgatact atcgcttccc gctagcgttc ttttcggaga
     4501 tggcaaggca gaacaatcgc cgggttcatc agttgccgcc gccggaagcc cggttcagga
     4561 tgtggcgctc cacatcgcac gcgagcagtt aagaatactt gcactgcctg gcgacgaaag
     4621 cgagcaggaa aagcgtctcg cttcggtgat atcgatattg cccaatatct tggccgcgct
     4681 cggcgacgac aaactcactc agaatttcgc cgccgcctgc aaagacaata cgccggcctc
     4741 catggtcgca ttcatcaagt gctgggtgat gggctcgaag gctgaggatg ttcaccctca
     4801 gaaaaatggc cgcgttccca aaagcctcgg cgccgtttcg aaagcgccag cgcccacctt
     4861 ttccgaagag ccagttcccg aaagcacaag attcaacggc gccgctcaca agccgcaagc
     4921 ccttaaagtc gatcaggcca aactcgatac gctgatgaat ctcatcgggg aactggtcgt
     4981 ctcgaaaaac agccttccgt tcctggcccg acgcgccgaa gaagtctacg gctcccggga
     5041 aatgtcgcgc gaaatcaagg agcagtacgg ggtcatcgac cgcttggcgc aggagatgca
     5101 gacagcgatc atgactgtgc gaatgcagcc ggtatcggag gcgttcgatc ggttcccccg
     5161 gctggtgcgc gacctcgccc ggaaactgtc aaagaaaatc gacttgcgga tggagggcga
     5221 ggaaaccgca gcggataaag cgatcatcgc cgcgctggga gagccgctct tacatattgt
     5281 gcgcaatagc atcgatcacg gcgtcgaaac gcccgaggag cgcatcgctg ttggcaaatc
     5341 cagtcaagcg gaaatcctgc taagggctta tcaggagtcc gaccagattg tgatcgaggt
     5401 gatcgacgac gggcgtggca tcgaccctgc caaaatcaag gccgccgcgt tgtccaaggg
     5461 cgtgatcacg gaggatcaag cagcgaggct ctcggatcag gagtcgatca acctcgtttt
     5521 ccaacccggc ttctccacaa cctcagaagt ttccgatctc tcggggcgcg gcgtg
//
LOCUS       DATMGX010000443         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_225638, whole genome shotgun sequence.
ACCESSION   DATMGX010000443 DATMGX010000000
VERSION     DATMGX010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225638"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..>2196
                     /locus_tag="VNR65_07160"
     CDS             410..>2196
                     /locus_tag="VNR65_07160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWJ18487.1"
                     /translation="MSPTPQREFHVVLIKPAHYDDDGYPIQWYRSAIPSNTLACLNGL
                     AMDAQGRSILGPDVAIQIHTFDETNRRMRPDKIIRMISERGGKALIGLIGVQSNQFPR
                     AVDIAAPFLKAGLPVCIGGFHVSGCIAMLPEWPKDMRDAQALGISFFAGEAEDGRFDQ
                     VVRDAWEGKLAPLYNFMDQLPSLQGQPVPILPQKHLSRTAGALSSMDLGRGCPYQCSF
                     CTIINVQGRKSRFRTPDDLEKIIRENYAQKIYRFFLTDDNFARNRDWEMHFDRMIELR
                     ANGFPKIGFTIQVDTLCHKIPNFIEKASQAGVRRVFVGLENINPDNLLAANKRQNKIT
                     DYREMLQKWRTYGAITCAGYIIGFPGDTKTSVLRDIEIIKRELPLDLLELFYLTPLPG
                     SEDHKRLLEKGVWMDPDMNKYDLNHRVTHHPKMSDTEWEEALKAAWASFYSLDHIRTI
                     LRRVAASPKGRPGTTLSTLLWFKLVHSYEGVHPLEGGALRLKFRRDRRSGLPIESPFI
                     FYPRYLGETVLKAWRYWSNYRQCNAIRKEVLAAPDRLTYTDTAIAPPKADEYEALDLY
                     HATSGAEGALARKHLHDDIRARAGAAAAVR"
BASE COUNT          467 a          676 c          612 g          441 t
ORIGIN      
        1 gaaccatccc ggcacgtcca gcaaggggca attgcttgcg ctgtcgcttg cgtgcacgat
       61 ggcggccgcg atcctctttc agccgcttct catggggccg ccgaggaaga aggcagaacc
      121 aagcccggtc cccgaaaaag aggcttcgtt gcaacagtag gcggcccgct cgctctccgg
      181 acaggcagat aagagcctga tctccaaaac gtgtttgggt aactgttggt aacggggcgg
      241 tcgagcacaa ctgtgtcccg tccgcacttg tctcgtccgt attcctgccg gaatgtcact
      301 cgttataggt cgagccggat gttttgggtg caaccgtgga tgccacgttt ccacccgatg
      361 gcgcgttttc gagcgttgcc gttctgcaca cgcaggtcag gaaggaaaca tgagcccaac
      421 cccgcagcgc gaattccacg tcgtgcttat caagcctgct cactatgatg atgatggcta
      481 ccccatacaa tggtatcgat cggccatccc ctccaacacg ctagcgtgcc tgaacggctt
      541 ggcgatggac gcgcaggggc gcagcatcct tggccccgat gtcgcaatcc agatccacac
      601 ctttgacgaa accaaccggc gcatgcggcc tgacaagatc atccggatga tctcggaacg
      661 cggcggcaag gcgcttatcg gcctcatcgg cgtgcaatcc aatcagtttc cgcgcgcggt
      721 cgatattgcc gctccgttcc tcaaggcagg ccttcccgtc tgcatcggcg gttttcacgt
      781 ctccggctgc atcgcaatgc tgccggaatg gccaaaggac atgcgcgatg cgcaggctct
      841 cggcatctca ttctttgccg gcgaggccga agacggccgc ttcgaccagg ttgtgcgcga
      901 tgcctgggag ggcaagctcg ctccgcttta taatttcatg gatcagcttc cttcgctgca
      961 gggccagccg gttcccatcc ttcctcaaaa gcatttgagc cggaccgcgg gcgcgctgtc
     1021 gagcatggat ctggggcgcg gctgccccta tcaatgttcg ttctgcacca tcatcaacgt
     1081 gcaagggcgc aagagccgct tccgcacgcc ggacgatctt gagaagatca tccgcgaaaa
     1141 ctacgctcag aaaatctatc gcttcttcct caccgacgac aatttcgccc gtaatcgcga
     1201 ttgggaaatg cacttcgacc gcatgatcga actgcgtgca aacgggtttc ccaagatcgg
     1261 atttacgatc caggtcgata cgctctgtca caagatcccg aacttcatcg aaaaggcgtc
     1321 gcaagccggc gtccgccgcg tctttgtcgg gttggagaac atcaatccgg acaatttgct
     1381 tgccgccaac aagcgtcaga acaaaattac ggactacagg gagatgctgc agaagtggcg
     1441 cacctacggc gctatcacct gcgccggtta tatcatcggg ttcccaggag acaccaagac
     1501 gtcggtcctg cgggatatcg aaatcatcaa aagggaattg ccgctcgatc tcctggaact
     1561 gttctacctg acccccttgc ccggttcgga ggatcacaag cgcctcttgg aaaaaggggt
     1621 gtggatggat ccggacatga acaaatacga cctcaaccat cgcgtcactc atcatcccaa
     1681 aatgagcgac acggaatggg aagaggcgtt gaaggcggca tgggccagct tctatagcct
     1741 agaccatatc cgcacgatcc ttcgccgggt tgccgccagc ccgaaagggc ggccagggac
     1801 aaccctgtcg acgcttctct ggttcaagct cgtgcattct tacgaaggcg ttcatccgct
     1861 cgaaggcggc gcgctccgcc tgaaattccg ccgcgatcgc aggagcgggc ttccgatcga
     1921 aagccccttc atcttctatc cgcgctattt gggcgaaacc gtcctcaaag cctggcgcta
     1981 ttggtccaat tacaggcaat gcaatgcaat ccggaaagag gtgctggctg cgccggatcg
     2041 attgacttat acggacaccg caatcgcgcc gccaaaagcc gatgaatacg aagcgctcga
     2101 tctctatcat gcgacttccg gggccgaggg tgcgctcgcg cgcaagcatc tccacgacga
     2161 cattcgggcc agagccggcg cggctgccgc agtgcg
//
LOCUS       DATMGX010000444         2410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_427885, whole genome shotgun sequence.
ACCESSION   DATMGX010000444 DATMGX010000000
VERSION     DATMGX010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427885"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..839
                     /locus_tag="VNR65_07165"
     CDS             99..839
                     /locus_tag="VNR65_07165"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HWJ18488.1"
                     /translation="MSGAYLGPHFAQAEIERRLIAAGAQFEVLGESEMIEATAQSLAA
                     QKAVGWFQGRMEFGPRSLGARSILGDPRSPGMQKNLNLKVKFRESFRPFAPAVLREDV
                     AEWFDLASDSPYMLIVADIKDGKRRHMTADEQALFGIEKLNIERSEIPAVTHVDYSAR
                     IQTVHSDTNPLFHKLLSRFKALTGCPILVNTSFNVRGEPIVCTPEDAFHCFMGSDIEV
                     LVVGNCFLNKEEQPTSLKRDYRNMFELD"
     gene            859..1005
                     /locus_tag="VNR65_07170"
     CDS             859..1005
                     /locus_tag="VNR65_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18489.1"
                     /translation="MKLPKVVQSLGAAAFASVVTLAALEVILRIVDLREARGRQRTLA
                     ELPI"
     gene            1073..1834
                     /locus_tag="VNR65_07175"
     CDS             1073..1834
                     /locus_tag="VNR65_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWJ18490.1"
                     /translation="MHHNSIGLRDDEFTLDAEPTIMVLGDSFVWGLDAEADERFTDLL
                     KPRVTGYKILAAGVSGYGTDQEYLLLQKLWPKVKPAVVVLIICTDNDRLDNSTNIRYE
                     GYQKPYFTIGADGALALHGQPVPKSRLQAIKEDWWVRHSWLVRLANAVYLKVRHPAVQ
                     VPDPTERLIDAIKKLVEANNAIFLVGLQKTDAGLVRHLQGRQIPFVAFDGAAAYPGEG
                     IGGHWTPEGHKLVAERLLALLLANPVVITKPLFGR"
     gene            1928..2140
                     /locus_tag="VNR65_07180"
                     /pseudo
     CDS             1928..2140
                     /locus_tag="VNR65_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009867220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          532 a          663 c          725 g          490 t
ORIGIN      
        1 agccggccgc gggcgatgcc ggcggtgcgg tgggatcggc gctagcggcc tatcatctct
       61 tcgagaacca gccgcgaaaa actaatggcg ccgacggaat gtccggcgcc tatctcggcc
      121 cacacttcgc gcaagccgag atcgaacggc gactgattgc ggcgggagca caattcgaag
      181 tcctcggcga gagcgagatg atcgaggcga cggcccagag cctggccgca caaaaggccg
      241 tgggctggtt ccagggccgc atggaattcg gtccgcgctc gctcggcgcg cgctcgatcc
      301 tgggcgaccc gcgctcgccc ggcatgcaga agaacctcaa tctgaaggtc aaatttcgcg
      361 agagttttcg gccgttcgcg ccggcggtac tgcgcgaaga cgtcgcggag tggttcgacc
      421 tcgcctcgga cagcccatac atgctgatcg tcgccgacat caaagacggc aagcgtcggc
      481 atatgacagc ggacgagcaa gcgttgttcg gcatcgaaaa actcaatatc gagcgatcgg
      541 aaatcccggc cgtgacccat gttgattatt cggcccgcat ccagaccgtg cacagcgaca
      601 ccaatccgct gtttcacaaa ctgctgagcc ggttcaaggc gctgaccggc tgtccgattt
      661 tggtcaacac cagcttcaac gtgcgcggtg agccgatcgt atgtacgccg gaagatgcat
      721 tccactgctt tatgggcagc gacatcgagg tgctcgtggt tggcaattgc ttcctgaaca
      781 aggaagagca gccaacctca ctaaagcgtg attacaggaa catgttcgaa ctggattaag
      841 cgggggcggc ataaaagcat gaaactcccg aaagtcgtgc agtctctggg agccgccgcg
      901 tttgcgtcgg tcgtgacgct ggcggcgttg gaggtcattc ttcggatcgt cgaccttcgc
      961 gaagcgcgag ggcgtcagcg aacgctcgct gagttaccaa tatgacgccg aactcgggtg
     1021 ggcaccggtc ccgggatcga gtgctagtgt tactaatgcg cgcaccattc aggtgcacca
     1081 caacagcatt ggcttgcgag acgatgagtt cacgctggat gccgagccga ccatcatggt
     1141 tctcggcgat tccttcgtct ggggcctcga tgccgaagcc gacgagcgct tcaccgacct
     1201 actgaagccg cgtgttaccg gttataaaat cctggcggcc ggggtatctg gttacggcac
     1261 cgatcaagag taccttctgc tacaaaaact gtggccgaag gtaaaaccgg ctgtcgtggt
     1321 tctgatcatc tgtaccgaca acgaccggct cgataacagc acaaacatcc gctacgaggg
     1381 ctaccaaaag ccctatttca ctatcggcgc tgatggcgcg ctggcattgc atggtcagcc
     1441 ggtgccaaaa tcacggctgc aggcaataaa agaggactgg tgggtgcgcc attcctggtt
     1501 ggtacgactc gccaacgctg tttatctaaa agtccgacat cccgcggtgc aagtgccgga
     1561 tccaaccgag cggttgattg acgccatcaa aaagttagta gaggcgaaca acgccatatt
     1621 cttggttggc ctacaaaaaa ctgatgcggg tctcgttcgg catctccaag gcaggcagat
     1681 cccgttcgtg gcatttgacg gtgctgcggc ctatccaggc gaaggaatag gggggcattg
     1741 gacacctgag ggacataagc ttgttgccga gcgcttgctt gccttgctgt tggccaaccc
     1801 cgtagtgata acaaagcctc tgtttggacg ctaatggtgc gcccccgaag gtcaaagtcg
     1861 cctcaccccc atctgagttg atttgaaaag ccgccttcgg gtggcttttc ttttactgct
     1921 tcctatcgtg cggttgatcc cgccggccta tgtgaagccc tatgtcaagc ggcagaagaa
     1981 tgacgcggag gccgtttgcg aatcagtcac cagggccaac gtgaggtttg taccaaccaa
     2041 gcagagtggc atggtgctgc atcgcgcgcg tcatctgtgc atgcgtcagc aaatctcggt
     2101 aatcaatgcg atacgcgccc atcttgccga gtttggaatt gtcgccgcgg tcggcggcaa
     2161 tggtgttgag gaactgctcg atatggtcgc tgatccaagt gacaagagag acgctcacga
     2221 acaggagcga aagaaagggc tttttttcag ttcgagatgg cgattgagca ggagttcgtg
     2281 gcgtgccgga ctgaggaaga tgcactcagg cagttgatga tggccgaggt ctgaaattgt
     2341 cccatctcag gcataccggc tggaataatc atcctcgccg agcaggaaat gggcaggaaa
     2401 gattgcccat
//
LOCUS       DATMGX010000445         3946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_252890, whole genome shotgun sequence.
ACCESSION   DATMGX010000445 DATMGX010000000
VERSION     DATMGX010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252890"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..200)
                     /locus_tag="VNR65_07185"
                     /pseudo
     CDS             complement(1..200)
                     /locus_tag="VNR65_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611875.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(641..811)
                     /locus_tag="VNR65_07190"
     CDS             complement(641..811)
                     /locus_tag="VNR65_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18491.1"
                     /translation="MVDQHQLNSIIATAIGDARKNHPDGRMDPEEAEQIAKCIVEALA
                     NAGLEIVPVSKD"
     gene            complement(1125..1427)
                     /locus_tag="VNR65_07195"
     CDS             complement(1125..1427)
                     /locus_tag="VNR65_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18492.1"
                     /translation="MSTKSRQMIYGGAIIGARIRAESARAAAKKAIRAADRAEAEAWS
                     IRMEGYGGPAQPSPTIAQCLNGSYCWMEIECNRCKTPCQHPARCHPPPARYPDLET"
     gene            1635..2000
                     /locus_tag="VNR65_07200"
     CDS             1635..2000
                     /locus_tag="VNR65_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18493.1"
                     /translation="MMRRSGWMPSIVPNGDDRNVYLVVDDFGRNGRAWREADVEATDL
                     ETVILDLLEGQYKNPVRVVGFNTAEGWSQDVSADVAHELRHRCDLQMRDVPFFLQDFV
                     DRYEGRYHDVQLPLPIRLV"
     gene            2326..2862
                     /locus_tag="VNR65_07205"
     CDS             2326..2862
                     /locus_tag="VNR65_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18494.1"
                     /translation="MPKLDLVNWTMRAAKQLRPEHHMRGDIEGPLSVSNILLGVLEGE
                     RGEPTSEGIASVRSVQLAGVNPIASPPSTPAEGRKPDTTSNLPADTQSAPLAPAGHAS
                     PMSPLLKAIVEPDIYTAPADRERAIVLRWVLRDIKANRLKLSPVDQLDLRDLIEMGLV
                     EMRGDVPLLTNAGDNAIL"
     gene            complement(3721..>3946)
                     /locus_tag="VNR65_07210"
     CDS             complement(3721..>3946)
                     /locus_tag="VNR65_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18495.1"
                     /translation="GMRARDLQRLRPAAAKPTSAERDPKTSLAAPDYRARMDLIFSMD
                     NQCELVRNSELRTNIERDAAFRDIAQDARD"
BASE COUNT          765 a         1139 c         1164 g          878 t
ORIGIN      
        1 tgaagctttc aatgaaggcg ttctgcatgg gtttgcccgg cgcgatgtag tgccagccga
       61 cgcgggcttg gtccgcccat gtcaggatgg cgttactggt aagctcgctg ccattgtcgc
      121 tgacgatcat cttcggcttg ccgctcgatc atcagccggt ccagttctcg cgccacgcgg
      181 acgccggaga gcgaggtgtc ccgccgcggt cgacgctcgt tatcatcggg ttcatcaccg
      241 cgtcgctctt atagcgatga tgcgtcgcct tccgggcaag gacgggcgcg gcagcaaaaa
      301 ccacaatcaa gatcgcaact agcgggcatc cttgttttcc ctttactatt gctacgcagc
      361 gtgaaacgga ccgggtaagg ttaagccgcg gatcagattg cggcggccga aaggctcgct
      421 atcctcctga gatgaccgac agtattgcat acgatttcta tcggggaaca gccgacggga
      481 gcctgcgaat agcgacgttg ccgggcgccg ggttgccggc gcacgttaag cgcaaggatt
      541 gggttctcat gcccaagggg aaaccgcctt tcccgttcga tatcgatcgc gacatagctg
      601 ttaagggcta ttgttttttc caactcgccg attgacgccg ttaatctttg ctgacgggga
      661 cgatttcgag gcccgcgtta gctaacgcct caacaatgca tttcgcaatt tgttcggctt
      721 cctcagggtc cattcggccg tccgggtgat ttttacgagc atcgccaatt gcagttgcaa
      781 ttatcgaatt cagttgatgc tgatcgacca tgcggtcctc ccggctatat gaggcttgct
      841 gtccaattgc cgatcatttc accgaccggg cgacatcggt cgccaagaca agtcgccgga
      901 ccacaggtag atgataatcg cagcgagaca taaggcaacg ccaatccaaa aagtcatcgt
      961 gtgatggacg tgtcgccaat cgggtgcgtg tttcattcga tcacctctct tcgtccgggt
     1021 gcacccaact gtagggcgta atctcacgcg tctcggtcag tttgatcatg tgcaccggtg
     1081 gcttatagcg gcgggtcccg caggaccggc atcggaacga tccttcaagt ttccaaatcg
     1141 gggtatcgcg cgggcggcgg atggcatcta gcgggatgct ggcacggggt cttacagcgg
     1201 ttgcactcga tttccatcca gcagtagctg ccgttgagac attgcgcgat tgtaggcgag
     1261 ggttgcgccg ggccgccgta cccttccatc cggattgacc aagcctcagc ctcggcgcga
     1321 tccgccgcgc ggattgcctt cttggcggct gcccgcgcgc tttcagcgcg tattcgggca
     1381 cctatgatgg cgccgccgta gatcatctgg cgggatttag tgctcatcag aggcggccca
     1441 atggaaagga cttgcccctt ttgggcacgt cctttccatt tagaacgcac cgcgaacgcc
     1501 aggcaatgcc actaggatta gcacatcgat tgctcgctga cgcactacag ccagcggtca
     1561 ggactggcac cgtgccttcc ggaacaagtt gtggcaggtt ttattgagtt cagggttctg
     1621 ttgcgtggga gttaatgatg cgtcgttccg gttggatgcc ctcgatcgtg cctaatggcg
     1681 acgatcgtaa tgtgtatctc gtcgtggatg acttcggccg gaacggccga gcatggcgtg
     1741 aagccgatgt cgaagcgacc gacctcgaaa ccgtgatcct cgatctgctt gaaggccaat
     1801 acaaaaatcc tgtccgcgtc gtcggcttca acacagccga aggctggtcg caggatgtat
     1861 cggcggacgt ggcgcacgaa ctgcgtcacc gctgcgatct tcaaatgcgc gacgtcccgt
     1921 tttttctgca ggatttcgtc gatcgttacg aaggccgata tcacgacgtt cagctgccgc
     1981 tgccgatccg ccttgtatga gcatggcttt ccagcgcaga aaaccgtgtc gccttcgaat
     2041 acgatgtaat agggcgatga ccgttggcag agcttgctat ttggcagaga aaaagccgcc
     2101 gggcgttaca ccgtcggctt tttcttgcga ctgccggctc gacctccggt gccgtcgcgg
     2161 ctttgagaag cctgcaacag catggttaac gaaaggttaa cgaaagcgga gtggcagcgg
     2221 cccgggggcc caaggccccg aacccaaaag ttcacccacg caacgagcca atttgtggca
     2281 tcattagact ttccgcccga ggtttgttgc gtggagcgtt tttttatgcc gaagttagac
     2341 ctagtgaact ggacaatgcg cgcggcgaag cagctgaggc ccgagcatca catgcgcggc
     2401 gacatcgagg gcccactctc cgtcagcaac atactgctag gggttcttga aggcgaacgg
     2461 ggggaaccga cctcagaggg cattgccagc gttcgcagtg tccagctggc cggggttaac
     2521 ccaatagcta gcccgccttc tacccccgcc gaaggacgca agccggatac cacttccaat
     2581 ttgccggccg acacgcagtc tgcgccgttg gctcccgcag gtcacgcctc cccgatgtcg
     2641 cccctcctga aggcgatcgt tgagccagat atctacacgg ctcccgctga tcgcgaacgc
     2701 gcgatcgtcc ttcgttgggt gttgagagat atcaaagcca atcggctaaa gttgtcgccc
     2761 gtcgatcagc ttgaccttcg agatttgata gaaatggggc tggtcgaaat gcggggcgac
     2821 gttccgctac ttacaaacgc gggcgataac gccatcctct gatcgatcat ccaatgggtc
     2881 agatcgaatt gaggaccgac tgaccctccg aaggataaga cgcgctgcag catggaaacg
     2941 ctcgcagcgc gggatggtgt tcaggacaca attccttgcc aagaccagta cgctacggtt
     3001 gtagggcggg cggtcgatga tattcaccat tcacgcttcg aaagccgatc agaacatcac
     3061 gacggtccgc ataggtcccg ccgcgactgc cgataaggtg cgcgaactag aaaagatggg
     3121 ctgggaactg atgcagcggg cagtcgattt acgccgccaa cttttgagca gctgctgttg
     3181 attgcccaag aagacagcgt agccttctgt ccggaccggg aaggcgccgc gtatccggag
     3241 cctttgtaaa gtttactgcc ggcccgatca accgtccggc gaacgatgcc ggggtcagcc
     3301 ttttctgcat cgcggatttg ccgagcttgg cggatttgct gctttgtcag cccatattcg
     3361 gccaacgttc ccagcgggtc gggattgatg acccgctgca tttttcccga tggccgtcgt
     3421 gagggccgac agcgtcccct cgatcctgtg ccgcgtcgta ttcgtcggcg aggcggcgct
     3481 tcgctagcgc ttcgatttcc agcgcgtcgg ctttcgcacg gtgggccgcg gcagtcagat
     3541 cgtcatgcgc cttcttcgcg cgatcgttgt cctagcgccg acgattttca gcttttgtta
     3601 gcgggcatag tgtaaactcc gatgcgttca attagccttg cccggaagcc catgggtgaa
     3661 ccccgcctat cactacggca ccgccttcta agaagtggct cgatccgctt tggcgacgcc
     3721 tcaatctctt gcgtcctgcg caatatctcg gaaggcggcg tcgcgctcga tgttagttcg
     3781 gagttcggaa ttccggacca attcacactg attgtccatg ctgaaaataa ggtccattcg
     3841 tgcgcggtag tctggcgcgg cgagcgacgt cttggggtca cgttccgctg aggtcggttt
     3901 agccgccgcc ggccggagcc tttggagatc acgcgcgcgc attccg
//
LOCUS       DATMGX010000446         3199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_223052, whole genome shotgun sequence.
ACCESSION   DATMGX010000446 DATMGX010000000
VERSION     DATMGX010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223052"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VNR65_07215"
     CDS             <1..557
                     /locus_tag="VNR65_07215"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HWJ18496.1"
                     /translation="QNYTFKYATSDKRESVELRFQKRMVQDILVNPPQFPGSRNVPIT
                     AAHLQNVLDPLSAIVLLSQARSNRGEPCNKRLPIFDGKIRYDLVLSPKGTRNVGTSGK
                     LRGTAYVCSVRFVPIAGHKMGKQGDNDVAAGNTGIEVWLVPVPEAGLIVPYYVHVPTP
                     AGTASMVTAKFDVQTPAGRHALAE"
     gene            726..>3199
                     /locus_tag="VNR65_07220"
     CDS             726..>3199
                     /locus_tag="VNR65_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWJ18497.1"
                     /translation="MSAASQTATIRPSQSRHVTAVLGPTNTGKTHLAIERMLGHESGL
                     IGLPLRLLAREIYDKIVARIGPRDVALITGEEKIKPEHPRYYVCTVEAMPRDVEVDFL
                     AVDEIQLAADPDRGHVFTDRLFHSRGMSETLLLGAQTMSDSIRELIPGANFIARPRLS
                     KLTYSGQKKITRLQSRSAIVAFSANDVYAIAELISRQRGGAAVVLGALSPRTRNAQVG
                     LYQSGDVDFLVATDAIGMGLNLDVDHIAFAGSRKFDGRVHRDLTPAEFAQIAGRAGRH
                     LNDGTFGVTADVEPFDQELVNKLENHDFEPVKVLQWRSTQLDFDSVDRLKDSLRQVPN
                     NSRLVRARNADDFETLEIACSDPRIAALAAGPAAVKRLWEVCQVPDYRKVALNSHAEI
                     VATLYKHIMSEEGLIPEDWFASQVEQADRTDGDIDTLSNRIAHIRTWTFVSNRREWLK
                     DPDHWQAKTRDIEDRLSDALHEQLTLRFVDKRTSVLMKSMRDKESLFAEIEKDGRVVV
                     EKHYVGKLEGFCFTPDLAAAGVHGKAARNAAAKVLVHELAARSDRLAAAPDTAFTLTR
                     EGKIVWEHQEIGGLAAGEDPLKPNFTLFSDEHLAAIDKENITKRIETWLGHHIEVRLK
                     PLVEIAKAEEIAGLARGIAFRLRENFGVLRRDTVADEIKSLDQDSRAQLRKYGVRFGA
                     HHIYFPLLLKPASADLLLLFWLLKHGSEHNLTAAAMSDAPRQGLTSVPRNAALPDEYY
                     RAAGFGVTGPRAVRVDMLERLADIIRPLTAWKPTEANTEPPLGAAGGGAFKIRPEMMS
                     IMGCSGEELSAILESLGFRREMRPVKR"
BASE COUNT          689 a          941 c          950 g          619 t
ORIGIN      
        1 cgcagaacta cacgttcaaa tatgcgacga gtgacaagcg ggaaagcgtc gagcttcgat
       61 tccagaaacg gatggtgcag gacattcttg tcaacccccc tcaatttcca ggctcgcgca
      121 atgtgcccat cacggcggcc catttgcaga acgttctcga tccgctcagc gcaattgtgc
      181 tcctgtcgca agcgaggtca aaccggggcg agccttgcaa taagcgtctt ccgatttttg
      241 atgggaaaat ccgctacgat ctggtcttat ccccaaaagg aacgcgaaac gtcggaactt
      301 ctggcaaact tcgtggaacc gcttatgttt gtagcgtgcg gttcgtgcca atcgctgggc
      361 acaaaatggg aaagcagggc gacaacgatg tcgcagctgg gaacacgggg atcgaagttt
      421 ggcttgtccc tgtgccagaa gcgggtttaa tagtaccgta ttatgttcat gtgccgacgc
      481 ccgcgggtac ggcatccatg gtgacggcga aattcgatgt gcagacacca gcggggcgcc
      541 acgcgctcgc cgagtgaaga ggggaacaat cgaggctcta ttgcgagaga ataagaagct
      601 gagcggtctc cagatttgga agacgcgctc gctctatttc cgcaggcgca cctcttcgta
      661 tgattaccgc gctaaagcct gaaagaattt ggacgaagcg tgcggttttg acagaaagtc
      721 cgcttatgag cgcagcatcg cagacggcta caataaggcc atcccagtcc cggcatgtaa
      781 cggcggtgct ggggccgacg aatacaggca agacccacct cgcaatcgag cgaatgctcg
      841 ggcacgagag cgggcttatc gggttgccgc tgcggctcct cgcgcgtgaa atctatgaca
      901 agatcgttgc gcggatcggg ccgcgcgacg tggcgctcat caccggcgaa gagaaaatca
      961 agcccgaaca ccctcgctat tatgtctgca ctgtcgaagc gatgccgcgc gatgtggagg
     1021 tggacttcct cgcggttgac gaaatccaac ttgcagccga ccccgatcgc ggccacgtgt
     1081 tcacggatcg tctcttccat tcgcgaggca tgagcgaaac actgctactc ggcgctcaaa
     1141 ccatgagcga ttcaatccgc gagttgatac cgggcgcaaa cttcatcgcc cgcccgcggc
     1201 tctccaagct cacctattcg ggccagaaga agatcacgcg gctgcaaagc cgatcggcga
     1261 ttgtggcatt ctcggccaac gatgtttatg cgatcgcgga actcatcagc aggcaacgcg
     1321 gcggcgcggc ggtcgtgctt ggcgcgctta gtcctcgcac acgcaatgcc caagtggggc
     1381 tctatcagtc gggcgacgtg gatttccttg tcgcgaccga cgccatcggc atgggcctca
     1441 atctcgatgt ggatcacatc gcattcgccg gctcccgcaa gttcgacggc cgcgtgcatc
     1501 gcgatttgac gccggctgag tttgcccaga tcgcgggccg cgccgggcgg catcttaacg
     1561 atggcacatt cggcgttacg gccgatgtgg agcccttcga ccaggaactc gtcaacaagc
     1621 tcgaaaatca tgatttcgag cccgtcaaag tacttcaatg gcgcagcaca cagcttgatt
     1681 tcgacagcgt cgaccgcctc aaggacagcc tccgccaagt gccaaataat tcgcgccttg
     1741 tccgggctag aaacgccgat gattttgaaa cgctggagat tgcctgcagc gatcctcgca
     1801 tcgcagccct tgctgcaggc cctgccgcgg tgaaacggct ctgggaggtc tgccaggttc
     1861 ccgattacag gaaagttgcg ctcaactctc atgcagagat agtggcgacg ctctataaac
     1921 atatcatgag cgaagaaggg ctgatccccg aggattggtt cgctagccag gtcgagcaag
     1981 ccgatcggac cgacggcgac attgacacgc tctcgaaccg catcgcccat atccggacct
     2041 ggaccttcgt ctccaatcgc cgcgaatggc tgaaagaccc cgaccattgg caggcaaaga
     2101 cccgggacat cgaagatcgg ctttcggatg cgctgcatga gcaattgacg ctgcgcttcg
     2161 tcgacaagcg cacgagcgtg ctcatgaaaa gcatgcgcga caaggagagc ctgttcgcgg
     2221 agatcgagaa agacggccgc gttgtcgtcg agaagcatta tgtcggcaag ctcgaaggct
     2281 tctgcttcac accggatttg gcggcggctg gcgttcatgg aaaagccgcc cgcaatgcag
     2341 ccgccaaggt gctcgttcac gagctcgccg cgcgttcgga ccgcctcgct gctgcgcccg
     2401 acacggcctt cacactcacg cgcgagggga aaatcgtctg ggagcaccag gagattggcg
     2461 ggcttgctgc gggcgaggat ccgctcaagc cgaatttcac gctcttttct gacgagcatc
     2521 tcgccgcgat cgacaaggaa aatatcacaa aacgcatcga gacttggctt ggccatcaca
     2581 tcgaggtgcg cctgaagccg ctggtagaga ttgcgaaggc cgaagagatc gccggtctcg
     2641 cccgcggtat cgccttccgc ctgcgcgaga atttcggcgt tctgaggcga gacacggtcg
     2701 ccgatgagat caagtctctg gatcaggaca gccgtgctca actcaggaaa tatggcgtgc
     2761 gtttcggcgc gcatcacatt tatttcccgc tgcttttgaa accggcgtcg gcagacctcc
     2821 ttctgctgtt ttggcttttg aagcatggga gcgagcacaa cctcacggcc gccgcaatgt
     2881 cggacgcgcc gcgccagggc cttacatccg tgccgcgcaa tgcagcactc cccgacgagt
     2941 actatcgcgc agccgggttc ggcgtgaccg gcccccgggc tgtgcgagtg gatatgctgg
     3001 agagacttgc ggatatcatt cgtccgctga cagcatggaa gcccacagaa gccaataccg
     3061 agccgcccct cggcgctgct ggaggcgggg ccttcaagat caggccggag atgatgtcca
     3121 ttatgggatg ctccggcgaa gagctgagtg cgatcctgga gtctctcggg ttccgccggg
     3181 aaatgcgccc cgttaaacg
//
LOCUS       DATMGX010000447         4244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_294608, whole genome shotgun sequence.
ACCESSION   DATMGX010000447 DATMGX010000000
VERSION     DATMGX010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294608"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VNR65_07225"
     CDS             <1..1248
                     /locus_tag="VNR65_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ18498.1"
                     /translation="ILLVANHMRRVFLPAVTTVSRAPQRYAAAARWVSLELGLATVVL
                     GLAGMLSQAKPAAHEQPYWWLPFRFAIDATWPVWPTPLWVVGGAVLAALSAAWLVGNR
                     QSMAAPQLAAVSAAGLVGMGVALWSLTVPAYPDTYLRSTVAYLTVSISEGKKHFERLC
                     TACHGPGGLGDGPAAKGLPKQPANLSEPHTALHTAGDMYWWLEHGIQPSAMPGFADVL
                     SDEDRWDVINFLRAFSQGFQARLLSPSIVSERPWLGAPGFYLDGDDDKARELKDFRER
                     RNVLLVFPDPGQERSLTRMRELSRDLDRMRTSGLVVLVISNMLDAGVSGSATIVRDGA
                     DEIRESYELLSRTLVNRGDGRSLGMKRNHMEFLIDRFGYIRGRWVADEEPNGWSPMDR
                     LLQQVDKLNTEPQILPAPDNHVH"
     gene            1281..1769
                     /locus_tag="VNR65_07230"
     CDS             1281..1769
                     /locus_tag="VNR65_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18499.1"
                     /translation="MTSKRACFFEMLPVLAVLAASPAVAQSNNEHMHHQHPGGDVMPN
                     ASEAPAQLPPLKILMPENGATVGTRLAIVFQTPADLSKMTMSAPVPGTHLHIDAQDVS
                     MMPSREQLVRFGEDRYLFLFDLPAKPGKNTLRVYWSDAQHRTIADTVQQITVTVARDA
                     PQ"
     gene            1969..2796
                     /gene="amoC"
                     /locus_tag="VNR65_07235"
     CDS             1969..2796
                     /gene="amoC"
                     /locus_tag="VNR65_07235"
                     /EC_number="1.14.99.39"
                     /inference="COORDINATES: protein motif:HMM:NF041641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial ammonia monooxygenase, subunit AmoC"
                     /protein_id="HWJ18500.1"
                     /translation="MTHALDVGTSGIVATNRATKDAAQHAAKDKYLNLGVTLMVMGGL
                     AMIYLAARVYQQMFAWSAGMDSTNPLFDTYWMTLLYTELTVLAIAAAVVWSYLWFSRD
                     RHLDKIEPKTELKRYFNLVAWILCYVFAVYFTASFFGEEDASWHQVVVRDTSFTPSHI
                     VLFYLTIPLYIILGIGSYLYATTRLPQFAKAISVPFVIGVTGPFLILPNLGFNEWGHA
                     FWLMEELFIAPLHWGFVVLGWSALALGGLLMQVTPNIIELLKKIYGAEEAEHLADTI"
     gene            3032..>4244
                     /gene="amoA"
                     /locus_tag="VNR65_07240"
     CDS             3032..>4244
                     /gene="amoA"
                     /locus_tag="VNR65_07240"
                     /EC_number="1.14.99.39"
                     /inference="COORDINATES: protein motif:HMM:NF014514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018597 - ammonia monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial ammonia monooxygenase, subunit AmoA"
                     /protein_id="HWJ18501.1"
                     /translation="MVAAAIFIVLVAAYHIHVELTIGDWDFWNDWKDREFWPTFTPVL
                     LITFPAAVHYILWTYFRLPIGATLCCVGLLLGEWIVRVHGFHIWAWFPYSLIWPATML
                     PGAIALDLVLLLGGNFIMSGIFGGMLFALLFYPGNWPMLAAYRLPLEHIGSLLTVGDM
                     IGYVYPRSSMPEYLRIIERGTLRTFGGHSAVVSAFFSGFMCILMYFVWWHIGKLLATT
                     AFVPSKLGKWMGLSDKTVSAPTATSSVVRPVTVVLGLAVLLTSVLYSMPTQAHGERNQ
                     EPFLRMRTVHWYDVKFSGTKAAVNDEITITGKFRIFEDWPEILPKPDTIFLGLASPGG
                     AMTRVESYIDGVPAIQSTGLQLGRDYDFKIVVKGRIPGRWHIHPMLNIQKAGPLPGPG
                     EWIDVTGNFNDF"
BASE COUNT          815 a         1240 c         1262 g          927 t
ORIGIN      
        1 atcttgctcg tcgccaacca tatgcgccgg gtcttcctgc cggctgtaac gacagtgtcg
       61 cgcgcgcccc aacgatacgc cgccgcagcg cgctgggtat cgctggaact gggcctggcg
      121 accgtcgtcc tcggccttgc cggcatgctg tcccaggcaa agcccgcggc tcacgaacag
      181 ccctactggt ggctgccatt ccgtttcgca attgacgcga cctggcccgt gtggccgacg
      241 ccattgtggg tcgtcggcgg ggccgtgctt gcggcgttat ccgcagcctg gctggtagga
      301 aaccggcaaa gcatggcggc gccacagctg gcagcagtca gtgccgcagg tctggtggga
      361 atgggagtag cgttgtggtc cctgacggtc ccggcctacc cggacacgta cctccgttcc
      421 acggtggcgt acttgacggt gtcgatttcc gaaggaaaga agcatttcga aaggctatgc
      481 accgcgtgcc acggcccagg cggccttggc gatgggccgg cggcgaaggg gctgccgaag
      541 cagccggcga atcttagcga accgcatacc gcgctgcaca cggcgggcga catgtactgg
      601 tggctggagc acggaattca gccaagcgcc atgcccggat tcgctgatgt cctgagcgat
      661 gaagatcgat gggacgtgat caatttcttg cgcgcttttt cccagggctt tcaggcccgc
      721 ctgctgtcgc cctcgatcgt ttccgagcgc ccctggctgg gcgcccccgg cttctacctg
      781 gacggcgacg acgacaaggc ccgcgagctc aaggactttc gggagcgaag gaatgtcttg
      841 cttgtctttc cagaccctgg ccaagagcgc agcctcacgc gcatgcgcga attgagtcgg
      901 gacttagatc ggatgcgaac cagcggcctg gtggtcttgg tgatatccaa tatgctcgat
      961 gccggcgtta gcggctccgc gaccatcgtt cgcgacggcg ccgacgagat ccgggagagc
     1021 tacgaactct tgtcgcgcac gttggtcaac cgcggcgatg gtcgcagcct gggcatgaag
     1081 agaaaccata tggaattcct gattgatcgg ttcggctata ttcgtggtcg ctgggttgcc
     1141 gacgaggagc ccaacggatg gagtccgatg gaccggctcc tccagcaggt agacaaactc
     1201 aacacggagc cacaaatact gccggcgccg gacaatcacg ttcattagtc gtgaatcaca
     1261 atgaacagag ggaggcgttg atgacttcga agcgggcctg ctttttcgag atgctcccgg
     1321 tgctagcggt actcgccgcg tcgccagcag tcgcgcaatc caacaatgag cacatgcacc
     1381 atcagcatcc cggtggggac gtgatgccga acgccagcga ggcaccggcg cagttgccgc
     1441 ccctgaaaat actgatgccg gaaaacggtg ccaccgtagg cactcgcctt gcgatcgtat
     1501 ttcagacacc ggccgacctg agcaaaatga cgatgagcgc tccagtgcca ggcacgcatc
     1561 ttcacatcga tgctcaggac gtatcgatga tgccgagccg agagcagctc gtccgctttg
     1621 gtgaggaccg ctatctgttc ttgttcgacc tgccagcgaa gcccggcaag aacacgttgc
     1681 gagtctattg gtcggatgcg caacaccgga cgatagcgga cacggttcaa cagatcacgg
     1741 tgaccgtcgc gcgtgatgcg ccgcaataat ttcttgcaga agcgtttcaa gcagcggcct
     1801 catgaaatcg cccatcttgt agttgagaat aactcgcatt ccgtcgcagt cttcccgtac
     1861 gaaaacagtt cacgggagtc agtttctatt gcttgttgac acaaaaggtt tgacgttgcg
     1921 tgtcgcagac tcatatcctg atctataaaa aatgggaggg aatgacgcat gacacatgct
     1981 ttggatgtgg gaacgagcgg tattgttgcg accaaccgcg cgaccaaaga tgcagcccag
     2041 cacgcggcca aagacaagta tttgaacctc ggcgtcacgc tcatggttat gggcggtctg
     2101 gcgatgatct acctggccgc cagggtctac cagcaaatgt tcgcctggtc cgcaggcatg
     2161 gattcgacca atccgctctt cgatacatac tggatgacgc tgctgtacac cgagttgacc
     2221 gttcttgcga ttgctgccgc cgtggtttgg tcgtatctct ggttctcccg cgaccggcac
     2281 ctcgacaaga tagaaccgaa gaccgaactc aaacggtact tcaatctggt tgcgtggatc
     2341 ctttgctatg tgttcgccgt ctattttacc gccagcttct tcggcgaaga ggacgcgtcc
     2401 tggcatcagg ttgtggtgcg cgacaccagc tttacgccta gccacatcgt cctcttctat
     2461 ctgaccatcc cgctctacat aatcctcggg atcggatctt acctgtacgc cacgacgcga
     2521 ttaccgcagt ttgccaaagc gatctcggtc ccgttcgtca tcggcgtgac cggaccgttc
     2581 ctcattctgc cgaatctcgg cttcaacgag tgggggcatg ccttctggct gatggaggag
     2641 ctgttcatcg ccccgctgca ttggggcttt gtcgttctcg gctggtcggc cttggcattg
     2701 gggggcctgc tgatgcaggt cactccgaac atcattgaac tgttgaagaa gatttacggc
     2761 gcggaagaag ccgagcatct ggctgacacg atctgacaac cgctgcggcc acggccggat
     2821 tccggacgca atatcttgca tccgtcaatc tctggccggg tcggcatcgg ccgcggggaa
     2881 ctgcaaaggc tgcgataacg aaacgccgca ataaccaata acgattccgc ggctgaccgg
     2941 gggggacaac gtggcaaaca tcacggagac tgcttggcgt ggggtgtggc cgtcaggcgc
     3001 ggccgcgaga gtatctcgga ccttcgattt tttggtcgct gctgcgatct ttatcgtgct
     3061 ggttgcagca taccacatcc atgttgaact gacgatcggc gactgggatt tctggaacga
     3121 ctggaaggac agggagttct ggccgacttt taccccggtg ctgttgatca cctttccggc
     3181 ggccgttcac tacatccttt ggacttactt ccgtctgccc atcggcgcca cactctgctg
     3241 tgtcgggctg ctcttgggcg aatggatagt gagggtccac ggcttccata tctgggcttg
     3301 gtttccctat tcgctgatct ggccggcgac catgttgcct ggcgccatag cgctcgattt
     3361 ggtgctgctc ctcggcggca atttcatcat gagcggcatt ttcggcggca tgctcttcgc
     3421 actgcttttc tatcccggaa actggccgat gttggctgcg taccgtttgc cgcttgagca
     3481 tattggatcg ctcctcacgg tcggagatat gatcggttat gtttatccac gcagcagcat
     3541 gccggaatat ctgcgcatca tcgagcgggg cacgctgcgc acattcggtg gccattccgc
     3601 ggtggtttcc gccttcttct cggggttcat gtgtatcctc atgtacttcg tctggtggca
     3661 tatcggcaag ctcctggcca cgacggcgtt tgtaccaagc aaactcggta aatggatggg
     3721 gctttcggac aagaccgtat cggcgccaac cgcgacaagc tctgtggtgc gaccggtaac
     3781 ggtcgtgttg ggtctcgcgg tgttgctgac cagtgttctg tacagcatgc ccacgcaagc
     3841 ccatggcgag cgcaaccagg aaccgttcct gcggatgcgt accgtgcatt ggtacgacgt
     3901 gaagttttcc gggaccaaag cggcggtgaa cgatgagatc acgatcaccg gcaagttccg
     3961 gattttcgaa gactggcccg agattctgcc caaacccgac acgatttttc tagggcttgc
     4021 ctcgccgggc ggggcgatga cgcgcgtcga atcctatatc gacggcgtgc cggcgatcca
     4081 gtcaaccggt cttcagcttg gccgcgacta tgacttcaag atcgtggtca agggccgcat
     4141 tcccggccgg tggcacatcc atccgatgct caatatccag aaagccgggc cactgccggg
     4201 ccccggcgaa tggatcgacg ttaccggaaa tttcaacgat ttcc
//
LOCUS       DATMGX010000448         3922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_332065, whole genome shotgun sequence.
ACCESSION   DATMGX010000448 DATMGX010000000
VERSION     DATMGX010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332065"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VNR65_07245"
     CDS             complement(<1..375)
                     /locus_tag="VNR65_07245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWJ18502.1"
                     /translation="MADGRLHCYLCPRHCHIGEGQSGFCFIRKNEGGRLLQLGYGRPA
                     AIAMDPVEKKPLSHFFPGTKILSMGTAGCNMGCSFCQNWDISKAKSDQVNALTLSAEN
                     VVDVALEHHAPHIAFTYNEPTIW"
     gene            727..2292
                     /locus_tag="VNR65_07250"
     CDS             727..2292
                     /locus_tag="VNR65_07250"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HWJ18503.1"
                     /translation="MPTAVEELPRTSTASSVVLDGHSLKIEDLVAIARDRYAIEVHPD
                     AIARIDKCRALLERKIRAREIMYGVNTGIGELSEVILTPEQAEKFQKYLLYSHAAGCG
                     EPCTEEDVRAGMISRLNTHCWGHSGIRNEIVQTQVAMLNKGVTPVVCQKGSVDACGDL
                     SPMSQMALTLMGEGEVFYEGRRLPAKQGMREAGIPTIVFRERDGLATINGSDLTTGIG
                     CLELFDATRWLKTQEVVLAMTLESLNANMMAYHPRIHATRGYPGSQECAENIRRLTEG
                     SELLNLPGKKVQDAYSLRSSPQVVGAAKEAFKWARYMLEIELNHAADNPTFFPDDDLV
                     LTGANFQGTPMAFALELVGMSVTTVAALSERRLNRLMNPHLSMGLPAFLTRGAGMFSG
                     LMLSQYTAGALICENRVLSHPAATGSIPAAADQEDFVSMGMTTAIKTRQIIENAWTVV
                     AIELMAAAQAFDLRTPVRPSPAAQAAYDVVRQHVSRLDEDRPLYEDINTLTRIAKEGA
                     ILHAAEEVAGKLN"
     gene            2607..>3922
                     /locus_tag="VNR65_07255"
     CDS             2607..>3922
                     /locus_tag="VNR65_07255"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HWJ18504.1"
                     /translation="MFFKPWNVRRNLYLVASLLLLSSLAYSAAPATMRLDYYHTGNAT
                     AEMFGFDRVVIEPLPWPGDLAKTIDDSNLGNYFFEVRDQASGRLLYSRGFGSIFSEWS
                     QTEEAKKLNRTFSESVRFPAPTAPVKITLKERKEGKDVSFHEVWSTTIDPKDKFIDRL
                     NPPSPGPLMTIQKMGEPATKVDLLILGDGYTAAERSKFEQDARRATEILFSRSPFREH
                     RRDFNVWALCPPAEQPGISRPSTGVLRHSPIGTSYDTFDSERYIMTTDNRALRDVASY
                     APYEFIEILANAKTYGGGGIFNLYATVAIDSEWAPYVFVHEFGHHFAGLADEYYTSDV
                     SYAPATQRIEPWEPNVTALLDPAILKWRDLVAPGTPLPTPWHKAEFEAYEHEIQAERR
                     RIRAENRPESEMDALFEKEKKHEDELLAADKYSDQVGAFEGANYESR"
BASE COUNT          858 a         1151 c         1155 g          758 t
ORIGIN      
        1 ccagatggtc ggctcgttgt aggtgaacgc aatgtggggc gcgtgatgct ccagcgccac
       61 gtcgaccaca ttctccgcac tgagcgtcaa cgcgttgact tggtcggact tggctttgga
      121 gatgtcccag ttctggcaga aggaacaccc catgttgcat ccggcggtgc ccatggaaag
      181 gatcttggtg cccgggaaaa agtggctgag cggcttcttc tcgacgggat ccatcgcgat
      241 ggcggcaggc cgcccgtagc ccagttgcag caggcgtccg ccttcattct tgcgaatgaa
      301 gcagaagccg ctctggcctt ctccgatgtg gcagtgccgt gggcagaggt agcagtggag
      361 acggccgtcg gccattgtct cccaccagcg cgcctcgtgg acttggggct gtaagactgt
      421 taactgcata gtaactggtt cttctcaatt gtaacgccga acggcgatcg ggcaccatgc
      481 ttctcgaccg aagtccgcac ctggggcaat tccaaaagtg gattgcacct tcttgggtag
      541 tgcgggcctt caggcccgcg ttaagcaccc tcccaattcc tcgctgctgg aaccgctcta
      601 aacacagcac accatccgcc ctccggacaa tcacgccatc aggtgatagc ggtcataccg
      661 caaggcgtca cagcggatca cattcgtttt gcggagatct tcacagccgc aacacaagga
      721 aggactatgc ccactgcagt agaagaacta ccgcgcactt cgacggcaag ctctgtcgtc
      781 ttggatggac actcactgaa aatcgaggac ctggtggcga ttgcccgtga ccgttatgcc
      841 atcgaggtgc atccggacgc catcgctcga atcgacaagt gccgcgctct cttagaacgg
      901 aagatacgcg cacgcgaaat catgtacggc gtgaacaccg gcatcggaga gctgtcggaa
      961 gtaatcctca cccccgagca ggcggagaaa ttccagaagt acctgctcta ctctcacgcc
     1021 gcgggttgcg gagagccctg caccgaagag gacgtccgcg cgggaatgat ttcccgcctc
     1081 aacacccatt gctgggggca ttcgggcatc cgaaacgaga tcgtgcaaac tcaggttgcg
     1141 atgttgaaca aaggcgtgac gcccgtcgtc tgtcagaaag gttcggtgga cgcgtgcggc
     1201 gatctttcgc cgatgtcgca gatggcgctg acactcatgg gcgagggcga agtattttac
     1261 gagggcaggc ggctgccggc gaaacaaggg atgcgagaag cgggaattcc cactatcgtg
     1321 tttcgcgaga gggatgggct tgccaccatc aatggctccg acctgacgac cggcatcgga
     1381 tgcctggagt tgttcgacgc gacacggtgg ctcaagacac aggaagtcgt gctggcgatg
     1441 acactcgaat cgctgaacgc caacatgatg gcctaccatc ctcggattca tgccacccgc
     1501 ggttaccccg ggtcgcagga gtgcgccgag aacattcgcc ggctaaccga aggctctgag
     1561 ttgttgaact tgcccggcaa gaaagtgcag gatgcctatt ccctgcgctc cagcccgcag
     1621 gtcgtcggag ccgccaaaga agccttcaag tgggcgcgct acatgctgga gatcgaactc
     1681 aaccatgcgg ccgataatcc caccttcttc ccggacgatg acttagtgct gacgggcgca
     1741 aatttccaag gtacgccgat ggcgttcgct ctcgaattgg tcggtatgtc ggtcaccacg
     1801 gttgcggccc tctccgagcg caggctcaac cgcttgatga atccgcacct gtccatgggc
     1861 ctgccggcat ttctgaccag gggcgcggga atgttctcgg ggttgatgct ctcgcagtac
     1921 actgcgggag cgttaatttg cgagaaccgc gtgctcagtc atcctgccgc cacgggctca
     1981 ataccggcag ccgccgacca ggaggatttc gtctccatgg gcatgaccac agccatcaag
     2041 acgcggcaga tcatcgagaa cgcgtggacg gttgtggcga tagagttgat ggctgcggca
     2101 caagccttcg atctgcgaac tccggtgagg ccgtcaccgg cggcgcaggc ggcctacgac
     2161 gtggttcgcc agcatgtcag caggctggat gaagacaggc ccctgtatga agacatcaat
     2221 accctgaccc gcattgcgaa ggaaggggcc atactgcacg cggcggaaga ggttgccggg
     2281 aagctaaact aagccagtac ttgctggcgc aatactccgc agagtgaggc tccgtggcga
     2341 atgcgcgtcc ccggcgaagc tacgtcggca gttgtactag tcagagcgga accagagttg
     2401 ctgtatccgg tattgggtgg agcacgcctt caggcgtgca taccaaggct gcaaaaaagg
     2461 cgaggcttta gccgctgagg taagggctct gattacctcc gcggctaaag ccgaattcag
     2521 acacaactct caaatgcacc gctgaagcgg tgctccaccc ggatacacca actcagaatc
     2581 tgtactaaga acgagagggt atcagaatgt tcttcaaacc gtggaacgtg cgacggaacc
     2641 tctaccttgt tgcctcgttg ctcctgttga gctcgctggc gtactcggct gctccggcca
     2701 ccatgcgact ggattactac cacaccggta atgctaccgc ggagatgttc ggctttgacc
     2761 gcgtggtcat cgaaccctta ccttggccgg gcgacctcgc caaaaccatc gacgacagca
     2821 atctggggaa ttacttcttc gaagtgcgcg atcaggcgag tggccgtctg ctgtactcgc
     2881 gcggcttcgg atccatcttc agcgagtggt cgcaaaccga ggaagcgaag aaactcaacc
     2941 gcacgttctc cgagtcagta cgctttcccg ctcccacggc tccagtgaag atcaccctca
     3001 aggaacgcaa agagggcaag gatgtaagct tccacgaggt gtggagcacg accatcgatc
     3061 ccaaagacaa gttcatcgac aggttgaatc cgccgtctcc gggcccgctt atgaccatcc
     3121 agaagatggg cgaaccggca accaaagtcg atctgttgat cctcggtgat ggatacaccg
     3181 ctgccgagcg aagtaagttc gagcaagacg cccggcgggc taccgaaatc ctgttctccc
     3241 gttcgccgtt ccgcgagcat cggcgggact tcaacgtttg ggcgctgtgt ccgccagcag
     3301 aacaaccggg catctcgcgg ccttcgacag gtgtgttgcg gcactcgccc attggcacgt
     3361 cgtacgacac gttcgacagc gagcgttaca tcatgaccac cgataatcga gcattgcgcg
     3421 atgttgcctc ctacgccccg tatgagttca tcgagatact ggcgaacgca aagacctacg
     3481 gcggaggagg aattttcaat ctatacgcta ccgttgcaat cgacagcgaa tgggcgccct
     3541 atgtttttgt gcacgagttt ggacaccact tcgccggact ggccgatgaa tactacacct
     3601 ccgacgtgtc gtatgctcct gcaacccagc gcatcgagcc ctgggagccg aatgtaaccg
     3661 ctctattgga tcccgcgatc ttgaagtgga gagacctggt cgcgcccggt acgccgctgc
     3721 cgacgccttg gcacaaagcg gaattcgagg cctacgagca cgagatccag gccgagcgcc
     3781 gccgcattcg cgccgagaac cggccggaat cagagatgga cgcgctgttc gaaaaagaga
     3841 agaagcacga ggatgagcta ctagcggctg acaaatattc ggatcaagtc ggcgcctttg
     3901 agggagcgaa ctacgaatct cg
//
LOCUS       DATMGX010000449         3405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_216795, whole genome shotgun sequence.
ACCESSION   DATMGX010000449 DATMGX010000000
VERSION     DATMGX010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216795"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VNR65_07260"
     CDS             complement(<1..1164)
                     /locus_tag="VNR65_07260"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HWJ18505.1"
                     /translation="MTDRILTINAGSSSLKFALFELNGRGPVLQVKGQVETLDTAPHC
                     ILKSSTGEVIENKMLPKHATDEHVEALLHVVDVLKAHVPGINILGVGHRVVHGGPNYS
                     APLILTEDIIADLEEYVPLAPLHQPHNLAGIAAAHVAFPEATQIACFDTAFHRAHPWV
                     NDTFALPRHYYEKGVRRYGFHGLSYEYVSSKLRQIAPDAASGRVVIAHLGAGSSMCGV
                     LNGRSIASTMGFSPLDGLPMGTRCGQLDPGVVLYLMDHEGLSSKEISDILYKHSGLKG
                     LSGLSGDMRELEAAGTPEAEQAIDYFVFRIRRELGGLAAALNGLDALVFSGGIGENSA
                     RIRHRICEDLHWIGLDLDDARNQSSDTRVSTLASRAQIFVIKTDEEAMIARHTI"
     gene            complement(1161..2558)
                     /locus_tag="VNR65_07265"
     CDS             complement(1161..2558)
                     /locus_tag="VNR65_07265"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HWJ18506.1"
                     /translation="MMRENKTYEEIRIGDEASIKRVLTANDLFIFAHASGNLNPMHLP
                     KESEDTVSDKIVAPSMWVGALISSVLGNVLPGAGTLYKGQTLKFLDRAHVGDQLTVKV
                     RVLEKRPGNVVTLATSVSRNGDTIAEGIAEVLAPVQKVELDDSQAPGVFVTRHKHFDA
                     LITKAKSLKPLRTAVVAPEAEHALAGAIMAAELGLIEPILVGDRRRIEEAAKAGGFKL
                     SRYEIVDASSGHDAAAKSVALVHSGDAASLMKGHIHTDELLHEVLKRDGGLRAGRRLS
                     HVFVMDVPGLEHLLLISDAAINIAPTLEEKVDITQNAIDLAIALGMVRPRVGVLSAVE
                     TVTPKIPSTLDAAILSKMADRGQITGGVVDGPLAMDNAIDMEAARTKGITSLVAGKAD
                     ILIVPNLEAGNMLAKELVFIAHADAAGIVLGARVPIILTSRADNEKARLASCAVAALY
                     RASQVNGGNAVQEAA"
     gene            2888..3274
                     /locus_tag="VNR65_07270"
     CDS             2888..3274
                     /locus_tag="VNR65_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18507.1"
                     /translation="MKRIIIAIAVTAIVPFAASGKEKGIHSEHASVSAYKPLQGFSHT
                     IGGRHFVGFFVATQKRCAVTVVDATVGNDPSFDGPRHQKFELAAGARSEIKADHGKAL
                     GIGCSSEANEITVVALEPNIPESANR"
BASE COUNT          704 a         1008 c          991 g          702 t
ORIGIN      
        1 gatcgtgtgg cgtgcgatca tcgcctcctc gtcggtcttg atgacgaaaa tctgggcgcg
       61 gctcgcaagc gtcgaaaccc tcgtgtcgct gctctgattg cgggcgtcat cgagatcgag
      121 gccgatccaa tgcaaatcct cgcaaatccg atgccggatg cgagcagagt tctctccaat
      181 cccgccagag aagacgaggg catcgagacc gttcaaagca gcggccagcc cccccagttc
      241 ccgccggatg cggaacacga aatagtcgat cgcctgctcc gcctcgggcg tcccggcagc
      301 ttcgagttcg cgcatatcgc cgctgaggcc ggacaagcct ttgaggccgg agtgtttata
      361 aaggatgtcg gaaatctctt tgctcgacag tccttcgtga tccatcagat agagcacgac
      421 gccagggtcg agctgcccgc accgcgttcc catgggcaag ccatcaagcg gtgaaaagcc
      481 catggtcgag gcgatactcc tgccattcag gacaccacac atcgacgagc ctgcgccaag
      541 atgggcgatc accacccgcc cgctagcagc atcgggcgcg atttgccgaa gctttgacga
      601 gacatactcg taggacaggc cgtgaaagcc gtagcggcgc acgccctttt cgtaatagtg
      661 gcgcggcaat gcgaaggtat cattcaccca cggatgggcg cggtggaacg cggtatcgaa
      721 gcaggcgatc tgggtggctt ccggaaaggc cacatgcgcg gcagctatcc cggccagatt
      781 gtgcggctgg tgcaagggcg cgagtggtac gtattcttca aggtcggcga tgatgtcctc
      841 ggtgaggata agcggtgcgg agtaattcgg accgccatgg acaacgcggt gcccgacgcc
      901 gagaatgttg ataccgggga catgagcttt gagcacgtcc acgacgtgaa gcaaggcttc
      961 tacatgctca tcggtcgcgt gcttcggaag catcttgttc tcgatcacct ccccggtgct
     1021 cgatttgagg atgcaatgcg gtgcggtgtc gagcgtttcg acttgtcctt ttacctggag
     1081 caccggaccc cggccgttca gttcgaacag cgcgaatttc agcgaggatg atcccgcgtt
     1141 gatggtcagg attcgatctg tcatgcggct tcctgtacag cattaccgcc attaacttga
     1201 gaggcgcgat aaagagctgc caccgcacag gaggcgagcc ttgccttctc gttgtcggcg
     1261 cggcttgtca ggatgatggg cacgcgagcg cccagcacga tgccggctgc atcggcgtgc
     1321 gcgatgaaca cgagttcctt cgccagcatg tttccggcct cgagattggg cacgatcaga
     1381 atgtccgcct taccggcgac aagcgaggta atgccctttg tcctggcggc ctccatatcg
     1441 atcgcattgt ccatggcgag cgggccatcc acgacgccgc ctgtgatctg tccgcgatcc
     1501 gccatcttgg agaggatagc ggcatccagc gtggacggta ttttcggtgt gaccgtctcg
     1561 actgccgaaa gaacgccgac gcggggcctt accattccga gcgctatcgc gagatcgatc
     1621 gcgttctggg tgatgtcgac tttttcctcg agtgtcggcg cgatattgat cgccgcatcc
     1681 gagatgagca ggagatgttc gaggccgggc acatccatga caaagacatg cgacaatcgc
     1741 cggccggccc gaagaccgcc gtccctcttg agaacctcgt gcaaaagctc atccgtatgg
     1801 atatggcctt tcatcagcga ggcggcatcg cccgaatgaa ccagcgcgac cgatttcgcg
     1861 gccgcgtcat gcccgctcga tgcatcgacg atctcgtagc gcgatagctt aaagcctccg
     1921 gccttcgccg cctcctcgat ccgccgccgg tcgccaacga ggatcggctc gatgagaccg
     1981 agttccgctg ccatgattgc gccagccagc gcatgctcgg cttccggtgc gacgactgcc
     2041 gtcctcagcg gcttcaggct ctttgccttg gtgatgagcg catcgaagtg cttgtgccgc
     2101 gtcacaaaca cgcctggcgc ttgcgaatcg tccagctcca ctttctgcac aggggccaac
     2161 acttcggcaa tgccttcggc gattgtgtca ccattgcggg agacgctggt ggccaacgtc
     2221 acaacattgc ccggcctctt ctccaggacg cggactttca cggtcaactg atcgccgaca
     2281 tgcgcgcggt ccaagaattt caacgtctgt cccttgtaaa gcgtacctgc cccaggaagc
     2341 acattgccga ggaccgaaga gatcagcgcg ccaacccaca tggagggcgc aacgattttg
     2401 tcggaaacgg tgtcctcact ctctttcggc agatgcatcg gattgaggtt tccggacgca
     2461 tgggcaaaaa tgaagaggtc gttcgcggtc agaacgcgct tgatcgaggc ctcgtcgcca
     2521 atccggattt cctcataggt cttgttttca cgcatcatga cggttctctt cctcagccac
     2581 tctggccgcg ccttggtgaa ggtttcgtcc gtattctggc ggcgagaaga atcggaacgg
     2641 caaaaggcca atgtcatccg cacggacggc gcggaacatt ttgcacggca agatcaaaca
     2701 cacgtccgac catttccgga acccgtaagg caaggtgtag ggcatttgta tgacagttca
     2761 gctaatggtt gccaaaagtt ctacctgagg atgtcataaa tatgttgcaa tgcaaaatta
     2821 gcaattctct agttatccta ataacacgaa agcctggggt gccccgtccc atgccgaaag
     2881 gacccatatg aaacgcatca tcatcgccat cgctgttaca gccatcgtcc catttgccgc
     2941 ctcaggcaag gaaaaaggga tccattctga gcacgcgtcg gtttcggcct acaagccatt
     3001 acagggcttt agccatacca ttggcggacg gcatttcgta ggattttttg ttgcaacgca
     3061 aaagcgctgt gccgttaccg ttgtcgatgc aaccgtaggc aatgacccca gtttcgatgg
     3121 gccgcggcac cagaagtttg aacttgcggc cggagcccgc tctgaaatca aggccgacca
     3181 tggcaaagcg ctcgggatcg gctgctcgtc ggaggcgaat gaaatcacgg tcgtcgcctt
     3241 ggaaccgaac attccagagt ccgcgaaccg ctaattaagg tcggcgtcct ggccgcgaac
     3301 gccgtggccg ggtgctccgc cattgtggac atcggcggat cgggcatcgg ggtaaaatgc
     3361 ctgatccgtc agcgacacct gcagcttcgt atgcgacgca attcg
//
LOCUS       DATMGX010000450         2817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_169317, whole genome shotgun sequence.
ACCESSION   DATMGX010000450 DATMGX010000000
VERSION     DATMGX010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169317"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="proB"
                     /locus_tag="VNR65_07275"
     CDS             complement(<1..890)
                     /gene="proB"
                     /locus_tag="VNR65_07275"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWJ18508.1"
                     /translation="MTFVNTHSVRPEWRNARRITVKIGSALLTDRATGALKQSWLEAI
                     GDDVAALKAEGRDVILVSSGAIALGRRALGLNAGPLRLEEAQAAASVGQINLAHAYQT
                     VFRERGLTTAQILVTLGDTEERRRYLNARNTIETLLKLGAIPVVNENDTVATSEIRYG
                     DNDRLAARVASMMTADCLVLLSDIDGLYTAPPAQDPNAKRLEVVTAITPEIEAMAGEA
                     ATELSRGGMITKLTAAKIAVAAGTHMVITTGKDLHPLRALGEGAPCTWFLAAANPVTA
                     RKRWIAGTLEPKGVVVIDDGA"
     gene            complement(924..1424)
                     /locus_tag="VNR65_07280"
     CDS             complement(924..1424)
                     /locus_tag="VNR65_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWJ18509.1"
                     /translation="MLDTMDIKILTILQEDSTLPVAEIGKKVGLSTTPCWRRIQKLEQ
                     DGVIQRRVALLDPKSVSAGVTVFVAITTNQHNAAWLDRFTDVVASFPEIVEAYRMSGQ
                     VDYLLRVAVPDISSYDRFYKAFISQIDLTDVSSSFAMEQIKFTTALPLSFAANAAAKR
                     PQQQAS"
     gene            complement(1532..2275)
                     /locus_tag="VNR65_07285"
     CDS             complement(1532..2275)
                     /locus_tag="VNR65_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWJ18510.1"
                     /translation="MRLTVVGCSDAFGSGGRYHSCYLLDTAQGRLMLDCGANSTLALK
                     RAGIALASIDAIVISHCHGDHFGGLPFVFLERMFMERGPKPVEILGPPGIAERTTDLF
                     EALYPTLPSLPMDFEIRYRELEPGHTSSWRGTQISAHEVVHFSGSPSLALTVSDGAKR
                     FAFSGDSAWCDGVIEAGRGADLYLVECTTFSTKTTVHLDYLTLAEKFESIGAKRYLLT
                     HMSPEMLAAGEKIDPGRCMLAEDGLSITI"
     gene            complement(2282..>2817)
                     /locus_tag="VNR65_07290"
     CDS             complement(2282..>2817)
                     /locus_tag="VNR65_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HWJ18511.1"
                     /translation="PFEQVDIMLGAHKSGDFAELNPIQRVPVLILNDGTAISESMAIC
                     RYFEAVQPDPPLFGSTPIEIGMIEMWNRRAELNFFQPVAQYFRHTHPAMAHLEVPQIP
                     AWAEANKPRVDEIIRIFDERLSAAPYLAGENFSVADITAMIATDFMKPARLTIPDEAL
                     NFKRWYAQVSSRPSHKA"
BASE COUNT          564 a          803 c          871 g          579 t
ORIGIN      
        1 gcgccatcgt cgatcacgac gacgcctttc ggctccagtg tgcccgcgat ccagcgcttg
       61 cgcgccgtga ccggattggc ggctgcgaga aaccaggtgc agggtgcccc ttcgccgagt
      121 gcgcgcaagg gatgcaagtc cttgccggtc gtgatgacca tgtgcgtgcc cgcggcaacc
      181 gcaatcttgg ccgcggtcag cttggtaatc attccgccgc gagacagctc cgtggcagct
      241 tcgccggcca tggcctcgat ctcgggcgtg atcgccgtta ccacttcaag ccgcttggcg
      301 ttggggtctt gcgcgggcgg cgcggtgtaa agtccgtcga tgtcggacag cagcacgagg
      361 caatcggccg tcatcatgct ggcgacgcgc gcggcaagcc tgtcattgtc gccgtagcgg
      421 atctcagatg tcgccaccgt gtcattctcg ttaacgaccg gaatcgcccc gagcttcaag
      481 agtgtttcga ttgtgttgcg cgcgttgagg tagcgccgcc tctcctcggt gtcgcccagt
      541 gtgaccagga tttgcgccgt ggtaagcccg cgctcacgga aaacggtttg ataggcatgc
      601 gccaggttga tctgccccac agatgccgcg gcttgcgctt cttcgagccg aagaggaccc
      661 gcgtttaggc cgagcgcacg ccgcccaagc gctatggccc ccgaagagac gaggatcacg
      721 tcgcgcccct cggccttcag cgccgccaca tcgtcgccaa tggcttccag ccaggattgc
      781 ttcagcgcgc ctgtggcccg atcggtcaag agcgccgagc cgatcttaac ggtgatgcgg
      841 cgcgcattgc gccattccgg ccgaacggag tgggtgttta cgaaagtcat gatgcgttga
      901 ggaacgccga ttttagcgag ccgtcaagaa gcctgctgct gagggcgctt ggcggctgca
      961 ttggcagcga aagacaaggg cagcgcggtc gtgaacttga tttgctccat ggcgaagctt
     1021 gaactcacat cggtgagatc gatctgcgag atgaacgcct tgtaaaagcg gtcatagctt
     1081 gaaatgtcgg ggacggcgac gcgcaggaga tagtcgacct ggccgctcat ccgataagcc
     1141 tctacgatct ccgggaagct ggcgaccacg tcggtgaacc ggtcgagcca ggcagcattg
     1201 tgctgatttg tcgtgatggc cacgaacacg gtcacgcctg cgctgacgct tttagggtcg
     1261 aggagcgcga cgcgccgctg gattacgcca tcctgctcca gcttttgaat gcgccgccag
     1321 cagggcgtgg tcgacaatcc tacctttttt ccaatttctg caacaggaag agtgctgtcc
     1381 tcctggagga tcgtgagtat cttaatgtcc attgtatcga gcatggggta tccgcaaaat
     1441 tcgttcgatg gccgtgctgt ctgcgaaaag tcaggctacg ctacagctaa ttatggcgga
     1501 agtatagaat aatttacccc aatcaacgaa attagatggt gatcgacaac ccgtcctcgg
     1561 ccaacatgca gcggccgggg tcaatttttt cgcctgccgc cagcatttca gggctcatat
     1621 gcgtgagcag atagcgcttg gctccgatgc tctcgaactt ttcggcaagc gtcagatagt
     1681 cgagatgcac ggtggttttt gtgctgaacg tcgtgcactc gaccagataa agatcggctc
     1741 cccggccggc ctcgatcacg ccgtcgcacc aggcggagtc cccagagaag gcaaagcgtt
     1801 ttgcgccatc gctcacagtc agggcgagcg acggagagcc ggagaaatga acgacctcgt
     1861 gggctgatat ttgggtgcct cgccatgagc ttgtgtgccc tggttcgagt tcgcggtagc
     1921 gaatctcgaa atccataggg agactgggaa gcgtgggata gagcgcttca aaaagatcgg
     1981 tggtgcgctc agcgataccg ggtggtccaa gaatctcgac cggcttcggt ccgcgctcca
     2041 tgaacatccg ttccagaaac acgaaaggca ggccgccaaa gtggtcgcca tggcaatggc
     2101 tgattacgat agcgtcgatc gatgccaatg cgatccctgc gcgtttcaac gccagcgtgg
     2161 agttcgcgcc acagtccagc atgagccggc cttgggcggt atcgaggaga tagcagctgt
     2221 ggtagcggcc gcccgagcca aacgcgtcgc tgcatcctac aaccgttaac ctcatgatgc
     2281 ctcatgcttt atgggagggc ctcgacgaca cctgcgcata ccatcttttg aagttcaagg
     2341 cttcatccgg gatggtaagg cgagcaggct tcataaaatc tgtcgcgatc atggcggtga
     2401 tgtccgcgac ggaaaaattt tcccctgcaa gatagggcgc agcgctcagc ctctcgtcga
     2461 aaatccggat gatttcatcc acgcgcggct tgttagcttc ggcccaggca gggatttggg
     2521 gcacttcgag atgagccatg gccggatgcg tgtgccggaa atactgagcc acaggctgga
     2581 agaaattgag ctcggcgcgc cggttccaca tctcgatcat gccgatttcg atcggcgtac
     2641 tgccgaacag gggagggtcg ggctgaacgg cttcgaaata gcggcagatc gccatgcttt
     2701 ccgagatggc agttccatcg ttcaggatca gaaccggcac gcgctggatg ggattgagtt
     2761 cggcgaagtc cccggacttg tgcgcgccca acatgatatc gacctgctcg aagggaa
//
LOCUS       DATMGX010000451         2724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_292303, whole genome shotgun sequence.
ACCESSION   DATMGX010000451 DATMGX010000000
VERSION     DATMGX010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292303"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VNR65_07295"
     CDS             complement(<1..419)
                     /locus_tag="VNR65_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ18512.1"
                     /translation="MINWALFEKFGPRMADGFLVTLELSFGSVLLGLLLATPVALGRV
                     FGSRPLSAALFGYTYVFRGTPILAQLFLLYYGSAQFQSTLSAAGLWWLFRDPFFCSLL
                     TFTLNTAAYQAEIFAGAIRSVPRGQIEAARAAAMPPAL"
     gene            complement(416..1093)
                     /locus_tag="VNR65_07300"
     CDS             complement(416..1093)
                     /locus_tag="VNR65_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ18513.1"
                     /translation="MDSTIIQWGHALALGAAMTVGLALATLPFGLALGLAAALAKDSK
                     SAIVRACGEIYTTVFRGLPELLTLLLIYYGAQILFQNMGLSVNISPFVAGLIALSLVF
                     GAYSSEVMLAALRGVERGQIEAARSFGMNPALVFRRVRAPQMLRLALPGLGNNWLVLL
                     KDTSLVSIIALDDLLRMSFIAAQSTRQPFQFYAVACAIYLAMTAISTMLIARAERAAA
                     KGWGTAA"
     gene            complement(1182..1676)
                     /locus_tag="VNR65_07305"
     CDS             complement(1182..1676)
                     /locus_tag="VNR65_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18514.1"
                     /translation="MKSGKKWGGGMKRAMIALGLAGLLAGCSTGSSGVENAALLNQPL
                     KGNTARVKIVRAETFAAALRGARLKLDGKQIADIGDGAATVLDIPAGAHEFTVDVWDH
                     PNVYKINVNAKPGMLYEMEVQPRSDAIVAGMFGVVGMAAEASANPNGGVFEIRVARES
                     RVAS"
     gene            complement(1758..2534)
                     /locus_tag="VNR65_07310"
     CDS             complement(1758..2534)
                     /locus_tag="VNR65_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWJ18515.1"
                     /translation="MLQARISRIVALTCVLVALSFAAPAQQSKLRIASEGTNPPFSII
                     NSSGELEGFDIDIARALCAKMQAECEIVAQDWDGIIPALLARKYDAIVASLNVTEDRK
                     NVVAFTKPYYRSPSAFVVRKGADVKDVSPTALIGKSIGVQSATNHSGFLEDVYSRSIV
                     RLYNTVADAYRDLAAGRVEYVLYDKLAIYDWLKSPEGACCEIAPAELNDPAYFGSGVA
                     IALRKGDDTLAQKFNSAIDGIVADGTYAKINAKYFPFPIF"
BASE COUNT          575 a          795 c          849 g          505 t
ORIGIN      
        1 agcgcgggcg gcatggcggc tgcgcgagca gcctcgatct gtccgcgagg cacgcttcgg
       61 atcgcgcccg cgaaaatctc ggcctgataa gcggccgtgt tcaatgtgaa ggtcaggagc
      121 gagcagaaga acggatcgcg aaagagccac cacagcccgg ctgccgatag tgtcgactga
      181 aattgggctg atccgtaata gagaagaaaa agctgcgcca ggatcggggt gcctcgaaaa
      241 acataggtgt atccaaaaag ggccgcgctt agcgggcgcg aaccgaagac acggcccagc
      301 gcgaccggtg tggcgagcaa aaggccgagc aacaccgatc cgaacgacag ctccagagtg
      361 acgagaaacc catccgccat gcgcggaccg aatttttcga agagcgccca gttgatcatg
      421 cggcggtccc ccagcccttg gctgcggcgc gctcggcgcg agcaatcagc atcgtcgaga
      481 tcgccgtcat ggcgaggtag atcgcgcagg caacggcgta gaattggaaa ggctgccgcg
      541 tactttgcgc ggcgatgaac gacatgcgca gaaggtcgtc gagcgcgatg attgagacaa
      601 gcgatgtgtc tttcagaagc acgagccagt tgttgccgag gccgggcagc gcgagccgca
      661 gcatttgcgg cgctctgact cgcctgaaca cgagagctgg gttcatgccg aaactgcgtg
      721 cggcctcgat ctgtccgcgc tcgacgcccc gcaacgccgc aagcatcact tcgctggaat
      781 aggctccgaa gacgagcgag agcgcgatca agcccgcaac gaagggcgag atgttgacgg
      841 acaaccccat gttctgaaac aggatctggg ctccgtaata gatgaggaga agcgtcagaa
      901 gctcaggcaa gccacggaag acggtggtgt aaatttcgcc gcacgctcgc acgatggcgg
      961 acttggagtc cttcgcgaga gccgccgcaa gcccgagagc aaggccgaaa ggaagcgtcg
     1021 ctaaagcgag ccccaccgtc atcgcggcgc ccagcgcgag cgcgtgtccc cattgaatta
     1081 ttgtgctgtc catgagagcc ggaatactgc cgcccaacgg acgggattag cttcgatttc
     1141 cgctcacatt agacgtattc gcgctgattg catcgcaaag ttcagcttgc aacccgtgat
     1201 tcgcgtgcca cgcggatctc aaacacgccg ccattcggat tggcagacgc ctcggcggcc
     1261 attccgacga ccccgaacat ccctgccact atggcgtcgg agcgaggctg cacttccatc
     1321 tcataaagca tgcctggctt ggcgttcacg ttgatcttgt agacattggg atgatcccag
     1381 acgtctacgg tgaattcatg ggcgccggcg ggaatgtcga gaacggtcgc tgcgccgtcg
     1441 ccgatgtccg cgatctgctt tccgtccaac ttgagccgcg cgccgcgaag ggccgcagca
     1501 aaggtttccg cccgaacgat cttgacccgc gccgtgtttc ctttgagcgg ctggttcagg
     1561 agcgccgcat tctcgacgcc agatgagccg gtgctgcacc cagccaggag accagccaac
     1621 cccaatgcga tcatcgcgcg cttcataccc ccgccccatt ttttgcccga tttcataaat
     1681 ccgtaatcga gtcgacagga gctgcctata gcgtggccga atttttccac gagctgtttg
     1741 tcccgtccgg gggctgccta gaaaatagga aatggaaaat acttggcgtt gatcttggcg
     1801 taggttccgt ctgcaacgat cccgtcgatg gcggagttga atttctgtgc gagcgtgtcg
     1861 tcgcccttgc gcagggcgat ggcgacgcca gacccgaaat aggccgggtc gttcaattcg
     1921 gccggtgcaa tctcacagca ggcaccttcg ggagatttca gccagtcata gatcgcgagc
     1981 ttatcgtaca gcacgtattc cacgcgaccc gctgcgagat cgcggtaggc gtcagccacg
     2041 gtgttataaa gccggacgat ggaacggctg tagacatctt caagaaaacc ggagtgattg
     2101 gtcgcgctct gaacgccgat ggatttcccg ataagcgccg tcggcgatac gtccttcaca
     2161 tcggcgccct tgcggacgac gaatgcgctc ggtgagcggt agtagggctt cgtgaaggct
     2221 actacgttct tgcggtcttc ggtgacattg agcgaggcga cgatcgcgtc atatttgcgg
     2281 gcgagcagcg cggggatgat gccgtcccaa tcctgggcga cgatttcgca ctccgcctgc
     2341 atcttggcgc atagcgcgcg cgcgatatcg atatcgaagc cttccagctc gccgctggaa
     2401 ttgatgatgc tgaagggcgg gttcgtgcct tcgctcgcga tccgcaactt cgattgctgc
     2461 gcaggagccg cgaaagagag ggcgacaagg acacaggtga gcgcgacgat gcgcgagatg
     2521 cgggcctgaa gcatggtgtt cgccgggcag tcttttattg agaattgccc ggcaccgtag
     2581 cccgctgctt atcggtcgta aagcgtcccg tccggaaaga cgcaaggaat tgctcaagcc
     2641 tggcgctctt gggcgcgttg aaaagctccg ccgatacgcc ttcctcctcg atccggccct
     2701 cggacaggaa aagggtgcgg gtgg
//
LOCUS       DATMGX010000452         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_97451, whole genome shotgun sequence.
ACCESSION   DATMGX010000452 DATMGX010000000
VERSION     DATMGX010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97451"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /gene="ppsR"
                     /locus_tag="VNR65_07315"
     CDS             <1..896
                     /gene="ppsR"
                     /locus_tag="VNR65_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010109 - regulation of photosynthesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator PpsR"
                     /protein_id="HWJ18516.1"
                     /translation="ARITEVNPAALALIGEPSKKIAAQTLLELIDPADGPALQDLLAM
                     VRSTGAGESIPVRLAGFDRKFIFSASFFRQGASAFILARLSPVETQAPEGAQKANASL
                     IKIMEGLPDGFVVTDPDSRILTANTAFLDLAQLSNQEQARGQPLENWLGRSGVDINVL
                     AANLREHSSVRNFSTIVRGAYGSLEEVDVSAVAVLGGELPCFGFVVRSSATRNVAKLR
                     PANLARSVEQLTELVGRVSLKDLVRESSDLIEKLCIEAALELTGDNRASAAEMLGLSR
                     QSLYVKLRRYGLGDLATEHGE"
     gene            973..1887
                     /gene="chlG"
                     /locus_tag="VNR65_07320"
     CDS             973..1887
                     /gene="chlG"
                     /locus_tag="VNR65_07320"
                     /EC_number="2.5.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF005742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthase ChlG"
                     /protein_id="HWJ18517.1"
                     /translation="MTAPAQTPPALTRPDLASVLEYMHPITWFAPMWAFMCGIVSSGA
                     SIGSHWQIFIAGILLAGPMVCGTSQAVNDWYDRHVDAINEPERPIPSGRIPGSWGFYI
                     AIIWTVLSLFLATALGPWGFGAATFGLILAWAYSAPPFRLKQNGWWGNLACGFAYEGM
                     PWFTGAAIMAGAFPDWRIVLFATLYSLGAHGIMTLNDFKSVEGDIKTGVRSIPVQLGV
                     KNAARLACVVMAVPQIIVIACLLAWDRPWHADLVGLSLAIQFFLMNRLLKNPKGEAIF
                     YNATGTTLYVLGMLVAAFALRPLIQGQM"
     gene            1887..>2245
                     /locus_tag="VNR65_07325"
     CDS             1887..>2245
                     /locus_tag="VNR65_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucC family protein"
                     /protein_id="HWJ18518.1"
                     /translation="MPNYLGWFGIIRLGLVQMSLGGIIALATSTLNRVMVVELALPAT
                     LPGLLFGIHYAAEMLRPRWGYGSDKGGTRTPWIVGGMLVLAVSGVSAAASIALMSTNT
                     AAGIACAILSYIGIGIGA"
BASE COUNT          398 a          678 c          659 g          510 t
ORIGIN      
        1 cggcgcgcat caccgaggtc aatccggccg cgcttgcgct gattggcgag ccgtcgaaga
       61 aaattgcggc ccaaaccctc cttgaactga tcgatccggc cgatggaccg gccctccagg
      121 atcttctcgc aatggtgcgg tccaccggag ctggcgaaag catccccgtt cgtctggcgg
      181 gcttcgatcg gaagttcatt ttttcggcgt ctttctttcg gcagggcgcc agcgccttca
      241 tcctcgccag gctttcgcct gtcgaaacgc aggcgcccga aggagcgcag aaagcgaacg
      301 cgtccctcat caaaatcatg gagggccttc ctgacgggtt cgtcgtcaca gatccggatt
      361 cgcgtattct caccgccaac accgcatttc tcgaccttgc ccagctttcg aaccaggagc
      421 aggcgagggg gcagccgctg gaaaactggc tgggccggtc gggcgtcgac atcaacgtcc
      481 tggcggcgaa ccttcgcgag cacagctccg tccggaattt ttcaacgatc gtccgcggcg
      541 cttacggttc cttggaagaa gtcgatgttt cagctgtggc agtgctgggg ggagaactgc
      601 cctgctttgg ctttgtcgtc cgctcttcgg cgacccgcaa tgtcgcaaag ttgcgcccag
      661 ccaatctggc ccgttccgtt gaacaactca ccgaacttgt cggacgcgtt tccttgaaag
      721 acctcgtccg ggaatcgagc gacctcatcg agaagctctg catcgaggcg gcgcttgagc
      781 tgaccggcga caatcgcgcc tctgccgcgg agatgctggg cctcagccgg caaagcttat
      841 acgtgaaact gcgccgctac gggctgggcg atctcgcgac tgagcacggc gaataaccat
      901 tttcgattta ttttttgcag caggttgtaa accaaaattg acaggtccgg ctgtaaaatt
      961 tagtgtccat gcatgacagc accagcccag accccgccgg cgctgacgcg accggacctc
     1021 gcttcagtcc tggaatatat gcatccgatc acgtggtttg cgcccatgtg ggcgttcatg
     1081 tgcgggatcg tgtcgtctgg tgcctcgata ggcagtcatt ggcaaatctt catcgcaggc
     1141 attctgctcg ctggaccaat ggtttgcggc accagccaag ccgtcaacga ttggtacgac
     1201 cgccatgtgg acgccatcaa tgagccagag cggcccattc cttccggacg gatccccggc
     1261 tcttggggtt tctatattgc aatcatctgg acggtcctgt ctcttttctt agcgactgcg
     1321 ctcgggccat ggggctttgg ggcagcgact ttcgggctca tcctcgcctg ggcgtacagc
     1381 gctccgccgt tccggctgaa gcaaaatggc tggtggggaa acctagcctg cggattcgcc
     1441 tatgagggta tgccatggtt cacaggcgcg gcaatcatgg caggcgcatt tcccgattgg
     1501 cgtatcgttt tgttcgccac tctttatagt ttgggcgcgc acgggatcat gacactgaac
     1561 gacttcaagt ctgtcgaggg cgacattaag actggcgtgc gttcgatccc cgtgcaactc
     1621 ggggtcaaaa acgcagcgcg gctcgcctgc gtcgttatgg cagtcccgca gatcatcgtc
     1681 atcgcctgcc tgctggcgtg ggataggccc tggcatgccg accttgtcgg attatcgctc
     1741 gccattcagt tcttcctgat gaatcggctg ctcaagaacc cgaagggaga ggcgatcttc
     1801 tataacgcga ccggcacaac gctctacgtg ctcggcatgc tcgttgccgc cttcgcgctc
     1861 cgtccgctga tacaagggca gatgtgatgc ccaattacct cggttggttc ggcatcattc
     1921 gtctcggcct cgtgcagatg tcgcttggcg gcattatcgc gctggccacc tccacgctaa
     1981 accgggtcat ggttgtcgaa ctcgccttgc ctgcgacgct tcccggcctt cttttcggga
     2041 tccattacgc cgccgagatg cttcggccgc gctggggata cggctccgac aagggaggaa
     2101 cgcgaactcc ctggattgtc ggcggtatgc tggtgctggc cgtatcaggt gtttcggcgg
     2161 cggcttcgat tgctctcatg tccacgaaca cggcagcggg gattgcttgc gctattctct
     2221 cctatattgg gatcggcatc ggagc
//
LOCUS       DATMGX010000453         2737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_312320, whole genome shotgun sequence.
ACCESSION   DATMGX010000453 DATMGX010000000
VERSION     DATMGX010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312320"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VNR65_07330"
     CDS             complement(<1..369)
                     /locus_tag="VNR65_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18519.1"
                     /translation="MERNDPPFALLDEAAISSAELRRDPYDFCFVENAIPESHKDEVL
                     ADAPNIPTHGSFGLPSLRYGPGFDAAVKDLLSVRFRRLVEQKFDMDLSPYPPCIVMMG
                     NTTGNYNEGYAHPDLKHKIVT"
     gene            complement(397..1677)
                     /locus_tag="VNR65_07335"
     CDS             complement(397..1677)
                     /locus_tag="VNR65_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HWJ18520.1"
                     /translation="MKAARASARKTPSSWGSGSKGAKSADALNILEQGVTVMLKVLSH
                     DGVTYRAFCGESGDEEYNLVVVPPAHLAFDDQIKFVQTAYADATKSLGLGPETAIFRR
                     LFLSDVMNQAALVRNSELVDDHGENPVAISIVQQPPLTGAKITLLAYHIVTKGQLSKK
                     RFTMKHVLVKKNGKRHLWSTRLCAGAFDPSPSSAGQTRKIFDNLTDTLSDLGGNLADH
                     CVRTWIYLRNVDVFYQGMVDSRRELFTHEGLTGSTHYISSTGIEGSCSHRFDVVSMDA
                     YSVLDLQPKQMSFLNDFDMLCATKDYHVTFERGTRIAYADRAHHFISGTASIDREGNV
                     LHVGDVVKQLDRAIVNVDALLRSGGAKLDDMQHLIVYLRDPTDYSRVTGMLNESFPGV
                     PTVIVQGAVCRPEWLVEIEGIAAAPHNDPALPAF"
     gene            complement(1839..2027)
                     /locus_tag="VNR65_07340"
     CDS             complement(1839..2027)
                     /locus_tag="VNR65_07340"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HWJ18521.1"
                     /translation="MEKNVGGVDRMLRIIVGLGLISLVFVGPHTPWGWIGVIPLLTAL
                     VSFCPAYRLIGMNTCSRA"
     gene            complement(2295..2516)
                     /gene="rpmE"
                     /locus_tag="VNR65_07345"
     CDS             complement(2295..2516)
                     /gene="rpmE"
                     /locus_tag="VNR65_07345"
                     /inference="COORDINATES: protein motif:HMM:NF001809.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWJ18522.1"
                     /translation="MKKDIHPDYHKITVVMTDGTQFETYSTYGAEGQKLQLDIDPATH
                     PAWTGGGHALVDRGGRVSKFKKKFEGFLK"
BASE COUNT          576 a          781 c          804 g          576 t
ORIGIN      
        1 ggtgacgatc ttgtgcttca aatcggggtg ggcgtagccc tcgttgtaat tgcccgttgt
       61 gttgcccatc atcacgatgc acggcggata ggggctgagg tccatgtcga acttctgctc
      121 gacaagacgg cggaagcgca cgctaagcaa atccttgacc gccgcatcaa accccggtcc
      181 gtaacgcagg cttggcaggc cgaagctgcc atgcgtcggg atattgggag catcggccag
      241 aacctcatcc ttatgagatt ccgggatcgc attttccaca aaacagaaat catacggatc
      301 gcgccttagc tccgcgctgg atatcgctgc ctcatccagc agcgcgaagg gtggatcatt
      361 gcgttccatg gcttggcctt ttctctctta agtaccctaa aatgccggca gtgcaggatc
      421 gttatgcggc gccgcggcga tgccctcgat ttcgacgagc cactcgggac ggcagaccgc
      481 gccttgcacg atcaccgtcg ggacccctgg aaagctctcg ttcaacatgc ccgtcacgcg
      541 gctgtaatcc gtcgggtcgc gcagatagac gataaggtgc tgcatgtcgt ccagcttcgc
      601 cccgcccgag cggagaagcg cgtcgacgtt gacgatggca cggtcgagtt gcttcaccac
      661 atcgccgaca tgcaggacgt tgccttccct gtcgatgctc gccgtgccgg aaataaagtg
      721 atgcgcgcga tccgcgtagg cgatgcgcgt gccccgctcg aaggtgacgt ggtagtcctt
      781 cgtggcgcac agcatgtcga aatcgttgag gaaggacatc tgtttcggct gcagatccag
      841 gacggaatag gcgtccatcg agaccacgtc gaaacggtgc gagcaggagc cttcgatgcc
      901 cgtgctggaa atgtagtgtg ttgagccggt cagcccttcg tgcgtgaaca actcgcgccg
      961 gctatcgacc atgccttggt agaagacatc gacgtttctg agataaatcc aggtcctcac
     1021 gcaatggtcg gcgaggttgc cgccgaggtc ggagagcgtg tcggtaagat tatcgaagat
     1081 cttgcgtgtc tgtcccgctg atgatggcga gggatcgaag gcgccagcgc aaagcctcgt
     1141 gctccacaag tggcgcttgc cgttcttctt tacgaggaca tgcttcatcg tgaaacgctt
     1201 cttcgagagc tgcccctttg tcacgatgtg ataggcgaga agcgtgattt tggcgccggt
     1261 caagggcggc tgctgcacga tggagatggc gacgggattc tcgccatggt cgtcgacgag
     1321 ttcgctgttg cggacaagcg ccgcctgatt catgacgtcg ctcaggaaca gccgccggaa
     1381 aatcgccgtc tcggggccaa gccccaaaga ctttgtcgcg tcggcgtagg ctgtttgcac
     1441 aaacttgatc tggtcatcaa aagcgaggtg cgccggcgga acgacaacca gattgtactc
     1501 ctcatcgccg ctttcgccgc agaacgcgcg ataagtcact ccgtcgtgag aaagcacctt
     1561 caacatcact gtcaccccct gttccaagat gttcagcgcg tccgccgatt tcgcgccttt
     1621 gctgccgcta ccccatgatg aaggtgtttt gcgggcgctg gcgcgggcag ccttcaaacg
     1681 ctccgccggt tgcggaaagc tgccctttga tcttggtcaa aattgctacc ttgcaggtaa
     1741 ccttcgattt agcgctgttc ggggacaaag ggcagagggg tgctggcgtg aagtcacaac
     1801 cagcagccct ttatccaaag gaaatgcagt tccgagcgct aagcgcgcga gcaagtgttc
     1861 atgccaatca accgataagc gggacaaaag ctcacgagag ccgtcagaag cgggataacg
     1921 ccgatccagc cccacggcgt gtgcggcccg acgaagacca gcgagatcaa ccccaaacca
     1981 acgatgatgc gcagcatgcg gtcaacgccg ccgacatttt tttccataac agcctccaag
     2041 tttcaggtcg tggagcttaa gcaggggatc gaggcggcgt ctgtgacttt gtcactatcg
     2101 cttaagccgt tgcacagggc cgaggaggcg ataggcaggg cagaagcccg cgaacgccgt
     2161 cgcttccgat agtgaggaga acgggggagc gcctgaaaat acgcacaacc cggcgcccga
     2221 gggttagccc ggtgcacaag tagatagaga ggctcgacgc ctccctgatc acgactgtgt
     2281 ctgaaaggca gcgcttactt caagaagcct tcgaacttct tcttgaactt gctgacgcga
     2341 ccgccgcggt cgacgagggc gtggccgccg ccagtccagg ccgggtgcgt cgccgggtcg
     2401 atatcgagct gcagcttctg cccttctgct ccgtaagtcg aataagtttc gaattgcgtg
     2461 ccatccgtca tcacaactgt gatcttatga taatcgggat ggatgtcctt tttcatggtc
     2521 tgttcccctt gcgcgtctat atgcgccttg tatctgctcc attatcgttg ctatacagga
     2581 cgaaatgaga tgatgacaag ttcaagcgtc atcgcgctgc tcttattcag gaaatccagt
     2641 ggcgcaggtg gacgcggtga gtgtaatccg tgacattcca agacatcgct gaacaccgtt
     2701 ccagcttgcc catagctgat gaaaccgcac aagccga
//
LOCUS       DATMGX010000454         3619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_351259, whole genome shotgun sequence.
ACCESSION   DATMGX010000454 DATMGX010000000
VERSION     DATMGX010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351259"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VNR65_07350"
     CDS             complement(<1..257)
                     /locus_tag="VNR65_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HWJ18523.1"
                     /translation="MPAGKAEASRERNRPLKVFVSERERAEIETRAAATGLSVSAYLR
                     NLGLGFQPHSTLDQEAILALLKVNADQGRLGGLFKLWLSGQG"
     gene            468..911
                     /locus_tag="VNR65_07355"
     CDS             468..911
                     /locus_tag="VNR65_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ18524.1"
                     /translation="MTNPSASPSSSDADVAGDTLQRIEVITGTGRRRKWSRRRKEAIV
                     LETLEEGASVSEVARRHGVDVSQVFKWRRDAGLRSRPSASEPVMFAPLLIEQKDAPQP
                     EQREATPLPSGLIELELAGARARIPVTAGREVILAVIDGLSMRRR"
     gene            908..1261
                     /gene="tnpB"
                     /locus_tag="VNR65_07360"
     CDS             908..1261
                     /gene="tnpB"
                     /locus_tag="VNR65_07360"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HWJ18525.1"
                     /translation="MIFGAVPPRIYIATRPVDFRKGLDGLAALVKEVLKLDPFCGAAF
                     IFRAKSADRLKILIWDGSGLILIYKRLDDATFRWPRIEDGVMRLSPAQASALFEGLDW
                     ARVRAVRRARPQAVQ"
     gene            1320..2906
                     /locus_tag="VNR65_07365"
     CDS             1320..2906
                     /locus_tag="VNR65_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWJ18526.1"
                     /translation="MIAVSEPLPDNIEALKALVLSARTELAAKDAELARSQDANARLW
                     ETLRQLRRAQFGRKSEKLDPDQLNLAMEETEQAIAEAGSAEETSNATLKASPKKPRSV
                     NRGKLPSHLPREEIVIEPESTACPCCGGALHVMGEDRSERLDVTPAQFKVIVTRRPKY
                     ACRACEDAVVQAPAPARVIEAGIPTENLVAHVLTAKYADHCPLYRQAQIYSRQGVDLD
                     RSTLADWVGRAAALLAPLQTRLFEILKASPKLFADETRCPVLDPGRKRVKRGQLWAYA
                     RDDKPWGGGDPPAVVYCYAPGRGSEHVKNHLAGFSGILQVDGYAAYKDFASSRPASEP
                     IILSFCWTHLRRRFYEIAVGGNAPLAEEALIQIAALYKIEETIRGESAEERRKVRQAK
                     SSPRLEKFKSWLEKTLTLVPGSSKMAKAIRYALHHWDGLILFANDGRIEMDTNTVERS
                     IRPLALNRKNALFAGHDRGADHWATVASLIETCKLNGVDPQAYLASVLSRLVNGWPMR
                     KIDELMPWAYAIPQNATAVA"
     gene            2872..>3619
                     /gene="traI"
                     /locus_tag="VNR65_07370"
     CDS             2872..>3619
                     /gene="traI"
                     /locus_tag="VNR65_07370"
                     /inference="COORDINATES: protein motif:HMM:NF041893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI/MobA(P) family conjugative relaxase"
                     /protein_id="HWJ18527.1"
                     /translation="MPSLKTLQPWPENTANVTIMIAKRVLRPRASSDFARLGAYILGE
                     GRSAPDQTFDYILADQAGSRVGAVRITNCDAGTPTLAIMEILATQSLNKRSLGDRTYH
                     LVVSFEPGERPAPAQIEDIETELCAAIGLSGHQRISALHLDRAHLHLHIAINKIDPAT
                     LRCIEPYYDKRKLMKACEELEIKHGLARTNHGRSHKGGPKGRAADFEAHSGQNSFASW
                     VMGKIEAPLLGLLAGACGWDDVHARLGKEGL"
BASE COUNT          683 a         1151 c         1126 g          659 t
ORIGIN      
        1 ccttgccccg acagccagag cttgaataag ccgccaagcc tgccctgatc ggcgttcacc
       61 ttgagaaggg cgagaatggc ctcctgatcc aaggtggagt gcggctgaaa tccaagcccg
      121 agattgcgca gataggcgga caccgacagg cccgtggcgg cggcgcgcgt ttcgatctcc
      181 gcgcgctcgc gctcggagac gaaaaccttg agcggccggt tcctctcgcg gctcgcctcc
      241 gccttccctg ctggcatggc ctcagccttc tcctttccgg cgcgcgtcag cgctctcgtc
      301 gagccgaagg cgagtaagaa agccggaggc cagctggccg aaggcggtga tgttggccaa
      361 catcacacat cttgccgtca aaatctttgt aagcgctgtt ccgacctcac cttccaattt
      421 tgattagacg atgcgagggt tggagcctgt gaggggggct tcgtcctatg actaatccta
      481 gtgctagtcc tagttctagc gatgccgacg ttgctgggga cacgcttcag cgcatcgagg
      541 ttattacggg gacggggcgc cgcaggaagt ggtcgcggcg gcgcaaggag gccatcgtgc
      601 ttgagacgct cgaggagggg gccagcgttt ccgaggttgc acgccggcac ggcgtcgatg
      661 tcagccaggt tttcaaatgg cgccgtgatg ctgggctgag atcgcgaccg tccgcttctg
      721 agcccgtgat gtttgcccct ttgctgatcg agcagaagga tgcgcctcag ccggagcaga
      781 gggaagcgac gccgctgcct tccgggctaa tcgaacttga acttgcaggg gcacgggcgc
      841 ggatcccggt gacggccggc cgcgaggtga tcctggctgt tatcgacggg ctttcaatga
      901 ggcggcggtg attttcgggg cggttccacc acggatctat attgcgacca ggcctgtcga
      961 tttccgcaaa ggcctagatg ggctcgcagc gcttgttaag gaagtgctga aactcgatcc
     1021 cttttgcggg gcggccttca tcttcagggc gaaaagcgcc gaccggctca agattttgat
     1081 ttgggacggc tccggcctga tcctgatcta caagcggctc gacgacgcga cgttccgctg
     1141 gccaaggatc gaggacgggg tcatgcgcct gtcgcccgcg caagcgtccg ccttgttcga
     1201 gggcctggac tgggcccgcg tcagggcggt tcggcgggcg cgaccccagg cggtgcaata
     1261 gcgctgatat ctttatgttt ttgcgtgcct tttcaggcaa atcatggtgc tgtcccgcca
     1321 tgatcgccgt ctcagagccc ttgccggaca atatcgaagc cctgaaggcg cttgtccttt
     1381 cggcgcgaac ggaactcgcg gcgaaagacg ccgaactggc ccgctcgcag gacgccaatg
     1441 cgaggctgtg ggagacgttg cgtcagcttc gacgcgcgca gttcggccgc aagtccgaaa
     1501 agctcgatcc cgaccagctg aacctcgcca tggaggagac cgagcaggcg atcgccgaag
     1561 cgggctccgc agaggagacc tcgaacgcga cgctgaaagc gtcgcccaag aagccccgct
     1621 ctgtcaatcg cggcaaactg ccgtcccatc tgccgcggga agaaatcgtc atcgagccgg
     1681 aaagcacggc ttgcccgtgt tgcggcggcg ccctgcatgt catgggcgag gaccgctccg
     1741 agcggctcga cgtgacgccg gcccagttca aagtcatcgt cacgcgcagg ccgaaatatg
     1801 cctgccgggc ctgcgaagac gcggtcgtgc aggcgccggc gccagcgcgt gtgatcgagg
     1861 ccggtatccc aaccgaaaat ctagtcgctc acgttctgac cgcgaaatat gccgatcact
     1921 gtccgttgta tcggcaggcg caaatctata gccgccaggg cgtcgatctc gatcgctcca
     1981 cgctcgccga ttgggttggc cgggcggcgg cgctcctggc tccgctgcag acgcgcctgt
     2041 tcgagatcct gaaagcctcg ccgaaactgt ttgccgacga gacacgctgc cccgtcctcg
     2101 atccgggacg caagcgcgtg aagcgcggcc agctctgggc ttatgcccgc gatgacaagc
     2161 cctggggcgg cggcgatccg ccggcagttg tttattgcta tgcgcctggc cgcggatcgg
     2221 agcatgtcaa aaaccatctc gcagggttca gcggcatttt gcaggtcgac ggctatgcgg
     2281 cctacaagga ctttgcaagc agccggccgg catcggagcc catcatcctg tccttctgct
     2341 ggacccatct gcggcggcgg ttctacgaga tcgccgttgg cggcaacgcg ccgctcgccg
     2401 aagaagcgct cattcaaatc gcagcgcttt acaaaatcga agagaccatc cgcggagaaa
     2461 gcgccgaaga gcgccgcaag gtccgccagg ccaagagcag tccgcggctt gagaagttca
     2521 agtcctggct cgaaaagacc ctgaccctcg ttcccggcag ctcaaaaatg gccaaggcca
     2581 tccgctatgc cctccaccac tgggacggtc tcatcctgtt cgcgaatgat ggacgcatcg
     2641 agatggacac caacaccgtc gagcggtcca tcaggcctct ggctctgaac aggaaaaatg
     2701 cactcttcgc cggccacgat cgtggagccg atcattgggc caccgtcgct tcattgatcg
     2761 aaacctgtaa gctcaatggc gtcgaccccc aagcctatct cgccagcgtt ctctcacgcc
     2821 tcgtcaatgg ctggcctatg cgcaaaatcg acgaactcat gccgtgggcc tatgccatcc
     2881 ctcaaaacgc tacagccgtg gcctgagaac accgctaacg ttacgattat gatcgcgaag
     2941 cgtgtgctgc gtcccagggc ttcctccgat ttcgcaaggc tcggcgccta catccttggc
     3001 gagggccgct ccgctcccga tcaaaccttc gactacatcc tcgccgatca ggccggcagc
     3061 cgcgtcggcg ccgtgcggat tacaaactgc gacgccggca cgccgacgct ggcgatcatg
     3121 gagatcctcg ccactcagag cctcaacaag cgaagcctcg gcgacagaac ctatcatctc
     3181 gtggtgtcgt tcgagccggg cgagcggccg gcgcccgcgc aaatcgagga catcgagacc
     3241 gagctctgcg cggcgatcgg gctgtcgggc caccagcgga tttcggcgct tcacctcgat
     3301 cgcgcgcatt tgcatctgca tatcgcgatc aacaaaatcg atccagccac cctgcgctgc
     3361 atcgagccct attacgacaa gcgcaagctg atgaaggcct gcgaagagct tgagatcaag
     3421 cacggcctgg cgcgcaccaa tcacggccgc tcgcacaagg gcggccccaa gggccgcgcc
     3481 gcggatttcg aggcgcatag cggccagaac tcgttcgcgt cctgggtcat ggggaaaatc
     3541 gaggcgccgc ttctcggtct cctcgccggc gcctgcggct gggatgacgt tcacgcgcgg
     3601 cttggcaaag aggggctcg
//
LOCUS       DATMGX010000455         3564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_308572, whole genome shotgun sequence.
ACCESSION   DATMGX010000455 DATMGX010000000
VERSION     DATMGX010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308572"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..768
                     /locus_tag="VNR65_07375"
     CDS             97..768
                     /locus_tag="VNR65_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ18528.1"
                     /translation="MRVLIIEDEARLAENVARSLRESAGYAVDVALDGEEGAFMAESN
                     PYDLVILDLMLPKLDGLTVLQRMRGGGSAVPVLVLTARDEKESIVALLNAGADDYVAK
                     PFDLGELLARAKALIRRGKGQSAAVLKVGDLEINTADLSVRRGGKLVTLTAMEYRVLE
                     YLAHRPGAVVPKTELLEHLYDFNWERFSNVIEVYVSGLRRKLEDSGDTKLIHTLRGQG
                     YMLRG"
     gene            770..2188
                     /locus_tag="VNR65_07380"
     CDS             770..2188
                     /locus_tag="VNR65_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWJ18529.1"
                     /translation="MRQTSIQRRLIAAVVVSQLLLAIGIVSVAVYFTRRQFRNGFDAG
                     LHGRAMSIAALVRYSEDEHPTLIFEDDVVPPPLEKQHPDLYQVTTGDGHVLARSPNWP
                     QNLQAVPKKDRQHANFTLDGIRYRAVRLENVPVLDREGDAPSTDVLTVTYAAPTDQMT
                     RAVAHAAIYIAAGSGLLLLITVALAVFGLRRGLRPLADLATSAALVNTSNWKLNPSEA
                     AVSTTELAPLTQAMTAMLDGLHRAFTQQREFLANAAHELKTPVAILKSTLQSLLQRPR
                     AAEEYRAGLEQALDDMARLEQLMHSMLRLARAEQWAAGSARRDLEAVEVAATCESALE
                     RLAPVARERGVTIHFASNGPMPMRADADDLELVWSNLLENAIRFSPAGGSVQLRMRAN
                     GQRGYIEVEDEGPGIPQSELARLFERFHRGDSSRARNTGGYGLGLAISKALIEAYGGT
                     ITPESGPGQGMRMVVEVPLEQG"
     gene            complement(2248..2430)
                     /locus_tag="VNR65_07385"
     CDS             complement(2248..2430)
                     /locus_tag="VNR65_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18530.1"
                     /translation="MPSKIRKPKPFRAASAVKAAARLAIGTPPPTRTEPASKKRKLKH
                     DKHKPTLNKLLGEAGE"
     gene            2915..>3564
                     /locus_tag="VNR65_07390"
     CDS             2915..>3564
                     /locus_tag="VNR65_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ18531.1"
                     /translation="MNRIQRFTGFVYEEVQMHCRQGQPDFIEIRIAPHGGMRGRCSLC
                     QRPAPGYDRLPERRWQFVPLWGIPTWFRYAPRRVKCAAHGVVVEQIPWSEGKRPVTCA
                     MMGFLAHWGRRLSWRETAQVFQTSWESVYRSVEWFVQWGLTHRQLQEVEALGVDEIHW
                     GHGLRADNFLTVIYQIDASCRRLLWVGKRRSEATLRQGLKALGPVVVQGLRFVCSD"
BASE COUNT          688 a         1026 c         1137 g          713 t
ORIGIN      
        1 cgatggtgtc aaaacgtaga tagggtgcgc gtcgcagggc ctcttaaggc ccccttcagc
       61 ttccctaccc tagcgttggc ataggatgga agcagcatgc gggtattgat catcgaagac
      121 gaggcccggc tggcggaaaa cgtcgctcgg agtttgcgcg aaagtgccgg ctacgcggtg
      181 gatgtcgcgc tcgacggcga agagggcgcg ttcatggcgg agtcgaaccc atacgacctg
      241 gtgattctcg atctgatgtt gccaaaactc gatggactta ccgtgctgca acgcatgagg
      301 ggcggtggct cggccgtccc ggtgctggtg ctcaccgcgc gcgacgagaa ggaatcgatc
      361 gttgcgctgt tgaacgctgg cgccgacgac tacgttgcca agccattcga cttgggagag
      421 cttctcgcac gcgccaaagc cctaatccgg cgtggcaagg gacagagcgc agcggtgttg
      481 aaggttggcg atctggaaat caacacggcc gatctcagcg tgcggcgcgg gggaaaactc
      541 gttaccctaa ccgcgatgga ataccgcgtt ctggaatatc tggcgcaccg gcccggcgcg
      601 gtggttccca agactgagtt gctcgaacac ttgtacgact tcaactggga acgattcagc
      661 aatgtgatcg aagtttacgt ttccggcttg cgtcgtaaac tcgaggacag cggagacact
      721 aagctgatcc acaccttgcg cgggcaggga tacatgcttc gtgggtaaca tgcgacagac
      781 ctccattcag cgccggttga tcgcagcggt agtggtctcg cagttgctac tggccatcgg
      841 catcgtttcg gtggcggtgt atttcacccg gcgtcaattt cgcaacgggt tcgacgccgg
      901 cctgcatggg cgggccatga gcatcgccgc gctggtgcgc tactccgaag atgagcatcc
      961 cacgctgatc ttcgaagatg acgtggttcc acccccgctg gagaagcaac atcccgatct
     1021 ctaccaggtg accaccggcg acggacacgt gctcgcccgg tctccgaatt ggccacagaa
     1081 tttgcaggcc gttccgaaga aggaccggca acacgcgaac tttacgctgg atggcatccg
     1141 ttatcgcgcg gtgcgactgg agaacgttcc cgtgctcgat cgcgaagggg atgcgccttc
     1201 gaccgatgtg ctgaccgtga cttatgcggc gcctaccgac cagatgaccc gcgcggtcgc
     1261 acatgccgcc atctacattg cggccggcag tggcctgtta ttgctgatca ctgtggccct
     1321 tgcagtcttc ggcttgcggc gaggattgcg tccactggcc gacctggcca ccagcgcggc
     1381 tttggttaac acctcaaact ggaagctgaa tcccagcgaa gcggccgtga gcacgacgga
     1441 actcgcgcct ctcacgcaag ctatgacggc catgctcgat ggattgcatc gcgcgttcac
     1501 ccagcaacgc gaatttctgg ccaacgccgc gcacgaactg aagacgccgg ttgcgatcct
     1561 gaagtccacg ctgcaatcgc tactgcaacg gccgcgcgcg gcagaagagt accgcgccgg
     1621 cctggagcag gcgctcgacg acatggcgcg cctcgaacaa ctcatgcact cgatgctccg
     1681 cctggcacgc gctgaacagt gggcggcggg cagtgcacgt cgcgacctgg aagcggtgga
     1741 agtggccgct acctgcgaga gtgcgctgga gcgccttgcc cccgtggcgc gcgagcgggg
     1801 cgtcaccatc catttcgcca gtaacggccc gatgcccatg cgtgccgatg ccgacgatct
     1861 cgaactggtt tggtccaacc tgctggagaa tgccatccgc ttcagccctg cgggcggaag
     1921 cgtgcaactt cgcatgcgcg ccaatggtca gcgcggctat atcgaggtcg aggacgaagg
     1981 tcctggaatt ccgcagtcgg agctggcccg cctcttcgaa cggtttcatc gcggcgatag
     2041 ctcgcgtgcg cgcaacaccg gcggatacgg cttgggtctc gccatctcca aagcgctgat
     2101 cgaggcctac ggcgggacca ttactcccga aagcgggccc gggcaaggca tgcgtatggt
     2161 ggtcgaggtg ccgctggagc aaggatgaaa aagtaggacg gagagcctta tgccgtggcc
     2221 ctccgaatcc gacactgcgg agttatatta ttccccagct tcgcccagaa gcttgttcaa
     2281 tgtgggcttg tgtttgtcgt gcttgagctt ccgcttcttg ctggctggct cggtccgcgt
     2341 gggcggagga gttccgatcg caaggcgagc cgcggccttc accgccgatg cggcgcgaaa
     2401 cggtttgggc ttgcgtattt tggacggcac gattcgtctc tcccggtcag tttgtgaccg
     2461 tcagcgaatg actttatatc acgagtgtca tgctaacgca gagagtaagc gcgccctgcc
     2521 ggacgcaccc aatcggcccg ctgaaccaag atcgttcagc cagattattc cggagcaact
     2581 attaatgtga atcaccccat tgattggcta ttgtcgcttt aacgccctct tcctatttag
     2641 ggctctttag cagaattcgt gggtaagagg gccgtggaaa aagccgctcc gtggaaaagt
     2701 ccaaaggcag gactttccca ctgcgcttga aaatcccgca aaagcggcgg gattgtcact
     2761 tttcccaccg ccccgacgac gactggttca ctctccaatt tcatcccgac aagaagaata
     2821 gctgaactcc agcaagctag cttagtctgc tgtttatcag gacagaaacc aagcagccgg
     2881 gagatcagct attcaactgc agattaagac catcttgaat cgaatacaac gttttacggg
     2941 ttttgtctac gaagaggtgc agatgcattg ccgccagggg cagccggact tcattgagat
     3001 ccggatcgcg ccgcatggcg gtatgcgcgg gcgctgctcg ctgtgccagc ggccagcccc
     3061 gggctatgac cgtttgccgg aacggcgctg gcagtttgtg ccgttgtggg gaatcccgac
     3121 ctggtttcgc tacgcaccgc ggcgggtgaa gtgtgccgcg catggcgtgg tggtggagca
     3181 gattccgtgg agtgagggga aacgtccggt gacctgcgcc atgatgggct ttctggccca
     3241 ctgggggcgg cggctgagct ggcgagagac ggcgcaggta ttccagacca gttgggaatc
     3301 ggtgtatcgc tcggtggagt ggtttgtgca atggggcctg acccaccgcc aactgcagga
     3361 ggtggaagcg ctgggcgtgg acgagattca ttggggccac gggttgcggg ccgacaactt
     3421 tctgaccgtg atctatcaga tcgacgcctc ttgccgacgt ctgctgtggg tcgggaaacg
     3481 gcgctcggag gcgacgctgc ggcaggggct gaaggcgctg gggccagtgg tggtgcaagg
     3541 gttgcgcttt gtctgtagcg acat
//
LOCUS       DATMGX010000456         2286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_329820, whole genome shotgun sequence.
ACCESSION   DATMGX010000456 DATMGX010000000
VERSION     DATMGX010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329820"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="VNR65_07395"
     CDS             complement(<1..1516)
                     /locus_tag="VNR65_07395"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HWJ18532.1"
                     /translation="MMQGQISDQARLAAHAVNKAGRSAVRIALRSPFSRWMAGVPTAY
                     HLLILPQDLRTGDPSFAVELYDGYFGLAGAAAPIGSESPFHVLPPSVPWQRDLYAFVW
                     LRHLHAADDQIAREKARKLTSDWFAFASGAPALARGRDITARRVIALLSHASFLLDGA
                     DPAFYDSVMRMLSRDLHDLTVVYNDGPGVGKIRALTAILLAGLCIAEQETYLSSYLGT
                     FTAELEKQVLGDGGHTSRDPSDLIELLLELLPLKQCFVARQIEPPPALYTAINRMNAM
                     LRFMRLGDGSLAHFNGMGPTLLDQVSTVLAYDDTGGMLSGVIAPQSGYARLAAGEVVV
                     IADAGGSPQMHLSSRAHAGCGSFEMSVGYEPLITNCGAPLDETGDWYVVSRSTAAHST
                     LTVDDLSSARLIKAQERVAGRELFWLAGPRSVHHDVADSDTALSVRIGHDGYREKFGL
                     LHRRRLTLRKSGLEFEGFDQLSAVGDNAIDPKARFAIRFHLHPRVAAQLSRNTNA"
     gene            1743..>2286
                     /locus_tag="VNR65_07400"
     CDS             1743..>2286
                     /locus_tag="VNR65_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18533.1"
                     /translation="MGQEGTTFNGDAAALIRAGPGSAVEESVSPQWLIDLKATADELR
                     QGRERPPGKPFGRWLGDFLDQDTTSGLLPAEEKLLFAAVNGEACVLESRAVLLWAAFD
                     SWRETMMPEAPAGDLPFVESIARFTEAAPEPVQEVIIEATRHAIEELRLPAYWEPKSV
                     ADVEKFALAQRAYLGRLEAEA"
BASE COUNT          487 a          663 c          732 g          404 t
ORIGIN      
        1 ccgcattcgt gttgcgcgaa agctgcgccg ccacgcgggg atgcagatgg aagcggatcg
       61 caaagcgcgc cttcggatcg atcgcgttgt cgccgacagc tgaaagctga tcgaaccctt
      121 cgaactcaag gcctgatttt cgaagcgtca gccgcctgcg atggaggaga ccgaacttct
      181 ccctgtagcc gtcatgcccg atccgaacgc tcaaggcggt gtcggaatcg gcaacgtcat
      241 gatgaacaga gcgaggcccc gcaagccaga aaagctcgcg gcctgcgaca cgctcctgag
      301 ccttgatgag ccgcgccgaa gacaagtcgt caacggttaa cgtggaatga gctgccgtag
      361 acctggagac gacgtaccaa tcgcccgtct cgtccagcgg cgcgccgcaa ttggttatga
      421 gaggctcata gccgacgctc atctcgaaag agccgcagcc cgcatgagcg cgcgagctga
      481 ggtgcatctg cggggaaccg ccggcatccg cgatgacgac gacctctccc gcggcaagcc
      541 ttgcataacc cgactggggc gcgatcacgc cggaaagcat gccgcctgta tcgtcgtaag
      601 caagcacggt cgagacctga tcgagaaggg tcggccccat gccattgaaa tgggcaaggc
      661 ttccatcgcc cagccgcatg aaacgcagca tggcattcat gcggtttatg gcggtgtaaa
      721 gagccggcgg cggttcgatc tgccgggcaa cgaagcattg cttaagcgga agcagttcga
      781 gcaaaagctc gatcaagtcg ctcggatcgc gcgacgtatg ccccccatct ccgaggacct
      841 gcttttcgag ctcggccgtg aacgtgccca agtaggagga gagatatgtc tcctgctccg
      901 caatgcatag gcccgcgagc aggatcgcgg tcaaagcgcg gatcttccca acgccaggac
      961 catcgttgta gacgacggtg agatcatgca aatcgcggct cagcatccgc atgacggaat
     1021 cataaaatgc aggatcggcg ccatcgagca ggaaggacgc gtgggaaaga agcgcgatca
     1081 cacgccgcgc cgtaatgtcc ctgccgcggg ccagcgctgg cgctccgctc gcgaaagcga
     1141 accagtccga ggtgagcttg cgagcttttt cccgggcgat ctgatcgtcg gcagcgtgaa
     1201 ggtgccgcag ccacacgaac gcatagagat ctctctgcca aggcacactt ggcggcagca
     1261 cgtgaaaggg cgattccgac ccgatgggcg cggcggcgcc agcgaggcca aaatatccat
     1321 cgtaaagttc gacggcaaag ctgggatcgc ccgtgcgcaa atcctgtgga aggataagga
     1381 ggtgataggc ggttggaacg ccggccatcc accgcgagaa cggcgatctt aaagcgatcc
     1441 tgacggctga tcgtcccgct ttgttgacag catgcgccgc aaggcgcgcc tgatctgaaa
     1501 tctgtccctg catcattaaa ggtttaggcg atcccctctc ttgcaggcaa ggcttctctc
     1561 gcccaccgcg aaacgaacga tggtaaggcc gggttgtgaa ccttaccaga ttcgcgctcg
     1621 ctttttcgga atactttgca gttaccaacc ggagcggacg cgtcatgaaa gccaatcatc
     1681 gatttagcat tccgactccc ttattaatat attaaggctt gatcgagtca tggcatcggg
     1741 gcatggggca agagggaacg acattcaacg gcgatgcggc cgctctgatc agggctgggc
     1801 cgggcagcgc tgtcgaggag tccgtctcgc cgcaatggct gatcgatttg aaggccacgg
     1861 cggacgagtt gaggcagggc cgcgaaaggc cccccggcaa gcccttcgga cgatggcttg
     1921 gggattttct cgatcaggat acgacgagcg gtcttctccc cgcggaggag aagcttctgt
     1981 ttgcggcagt gaatggcgag gcctgcgtgc tggaaagccg ggccgttctc ctgtgggctg
     2041 cctttgattc gtggcgggag acgatgatgc cggaggcgcc ggctggggac ctccccttcg
     2101 tcgagtcgat cgccaggttt acagaggctg cgccggagcc cgtgcaggaa gtcatcatcg
     2161 aggcgacgag gcacgcgatc gaggagctga ggcttccggc gtattgggag ccaaagagcg
     2221 tggccgacgt ggaaaagttc gcattggcgc agcgcgctta ccttggccgg ctggaggcgg
     2281 aggccc
//
LOCUS       DATMGX010000457         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_382713, whole genome shotgun sequence.
ACCESSION   DATMGX010000457 DATMGX010000000
VERSION     DATMGX010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382713"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VNR65_07405"
     CDS             <1..899
                     /locus_tag="VNR65_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWJ18534.1"
                     /translation="DAYPADPSTTVVAMPSFDVSTFQAGAFNKDASTPSIELDTQHKH
                     GIVRRSVIRTLEDQKEIYMAPFRLSNLRWDALVLAPTAVFLVTDRHIENNLPGGHYTF
                     YQDTSDVAIGGLAATLAGVWIYGIRGDHPHARETGNLVLETLVNTFLIYAPMQFIAGR
                     QRPGEGNGHGDFWEHHAINTSFPGGHAMFTWAMATVVAQEYPKPWVQILNYTAAFTVT
                     TTRFLARDHWASDMWVGAALGVAIGTHVFHAHCDPELSASCMRHHQKMLKRKANLAKS
                     ALYAWPNAKPTEDYASGRPISQ"
     gene            complement(869..1264)
                     /locus_tag="VNR65_07410"
     CDS             complement(869..1264)
                     /locus_tag="VNR65_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ18535.1"
                     /translation="MTDRSAVHTTIAPWLSVRDGAGAVDFYKSAFGAIEVFRMDAGDS
                     VIARLSVEGAEFWLSDESPEHGNFSPQSLNGSTVRIILTVVDPDAVFARALAAGASQV
                     YPVTEEHDWRLGRVVDPFGHHWEIGRPLA"
     gene            1433..>2240
                     /locus_tag="VNR65_07415"
     CDS             1433..>2240
                     /locus_tag="VNR65_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HWJ18536.1"
                     /translation="MKSRSLPFLRVASFVCVGVIVVGLAPWAGAQQPLASHLTKRVVA
                     DYVYWSKYNNPPYSAAQIPYRKLTHINHAGVSFDATGTLTVPQGFIEPELNNGAHAAG
                     VKVLLLLGGDFVGLEASGAMQTLVDNVAALETQYGYDGADIDWEYPASSGDRAFMVNL
                     MASLRASNPNYVLSIDIAPWGGYGYDLRLLQLSLDYFNIMMYDCAGPWTAHGQLNSPI
                     FWDPHDPAPYECQPGGSVEQTSDYFVKQVPGAQLNMGTPFYGYHYANIHQV"
BASE COUNT          465 a          598 c          724 g          453 t
ORIGIN      
        1 ccgatgcgta tcctgcggat ccgtcaacca ctgtggttgc gatgccgagt tttgatgtct
       61 cgacatttca ggccggagca tttaataagg atgcttctac acccagcatc gaactggaca
      121 ctcagcacaa gcacggaatc gtgagaaggt cggtcatccg gacactggaa gaccagaaag
      181 agatctacat ggctccgttc aggctctcga acttgcggtg ggatgcgttg gtgctggcgc
      241 cgacggcggt cttcctggtg accgaccggc acattgagaa caatctgccg ggcggtcatt
      301 acacttttta tcaggacact tccgatgtcg cgattggagg actggcggct acgctagccg
      361 gggtttggat ttatgggatc agaggcgacc atcctcatgc aagagagacg gggaacctgg
      421 tactggagac gctggtcaac acgtttctga tctatgcgcc gatgcagttc attgcagggc
      481 ggcagcgtcc gggggaaggc aacggacacg gagacttctg ggagcatcac gcgatcaaca
      541 cgtcgtttcc cggcgggcac gcgatgttca catgggcgat ggctacggtg gtcgcgcaag
      601 agtatccgaa gccatgggta cagattctga attatacggc tgcgtttacg gtgacgacga
      661 ctcgctttct ggcgcgcgat cactgggcgt cggatatgtg ggtgggagcg gcgctggggg
      721 tggcgatcgg cacgcatgtg tttcatgcac attgcgatcc ggagttgagc gcatcgtgca
      781 tgcgccatca ccagaagatg ttgaagcgga aagcgaatct ggcgaagtcg gccctctatg
      841 cgtggccgaa tgcgaagcca acggaagatt acgcgagcgg gcggccgatt tcccagtgat
      901 gtccgaaagg atcgacgaca cggcctaagc gccagtcgtg ctcctcggta acgggataca
      961 cctgcgacgc gccggctgcg agggcgcggg cgaagacggc gtcgggatcg acgaccgtta
     1021 agatgatgcg gaccgtgctg ccgttaagac tttggggact gaagttgccg tgttcggggg
     1081 actcgtcgct caaccaaaac tcggcgccct ccacggagag gcgagcgatc acgctgtcgc
     1141 ccgcatccat gcgaaacact tcgatcgcgc cgaaggcaga cttgtagaag tccacggcac
     1201 cggcgccatc gcgaacggag agccaggggg cgatggtggt gtggacggcg gaacgatcgg
     1261 tcataaaaag ctcctcgtga aaaataacgc agactgattc ctactggaaa acagtactgg
     1321 aaaacagtac cagccgattg cagagttgtc gtggtagaat cctcgaactt tcggtcggac
     1381 accactttgt acgagctgtc cgggtcttaa gcctcctctc aggggtgcat ccatgaaatc
     1441 tcgttccctt ccgttcctgc gtgtggcatc gtttgtatgc gttggtgtga tagtggtggg
     1501 tcttgcgccg tgggcgggcg cgcaacaacc gctggcaagc catctgacga agcgcgtggt
     1561 cgccgactac gtgtactgga gcaagtacaa caatccgccg tacagcgcgg ctcagattcc
     1621 ctaccgcaag ctgacgcaca tcaaccatgc gggagtttcg tttgacgcga cgggcacgct
     1681 gactgtgcca cagggattca ttgagccgga attgaacaac ggcgcgcacg ctgcgggtgt
     1741 gaaggtgctg ctgctgctgg gaggcgactt cgtgggactc gaggccagcg gcgcgatgca
     1801 gacgctggtg gacaacgtgg cggctctcga gacgcagtat ggttacgacg gcgccgatat
     1861 tgactgggaa tatccggcgt cgagcggcga tcgcgccttc atggtgaacc tgatggcgag
     1921 cctgcgggcg tcgaatccga actatgtgtt gtcgatcgat attgctccgt ggggcggcta
     1981 cgggtacgac ctgcgcctgc tgcaactgtc gctcgattat ttcaatatca tgatgtacga
     2041 ctgcgccggg ccgtggacgg cgcacgggca gctcaattcc ccgatcttct gggacccgca
     2101 cgatccggcg ccgtacgagt gtcagccggg aggaagcgtg gaacaaacct ccgactattt
     2161 cgtgaagcag gttccgggtg cgcaactgaa tatggggacg ccgttctacg gctatcacta
     2221 tgcgaacatc catcaggtgt
//
LOCUS       DATMGX010000458         4434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_221024, whole genome shotgun sequence.
ACCESSION   DATMGX010000458 DATMGX010000000
VERSION     DATMGX010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221024"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VNR65_07420"
     CDS             complement(<1..543)
                     /locus_tag="VNR65_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18537.1"
                     /translation="MPVFFISDTHFGHENIIRMCRRPFASVADMDEALVARWNERVGA
                     ADTIYHLGDFCYRNSRGADTYLRQLNGRIHLIAGNHDDETIAHHAPLFASVSHIGEIH
                     ERGHRIVLCHYPMREWHGSWNKSWHLFGHVHGRLDHEPHGYSLDVGADAHDFRPWSLD
                     EIEEEFAERTDPFSEGRIRPR"
     gene            complement(624..1037)
                     /locus_tag="VNR65_07425"
     CDS             complement(624..1037)
                     /locus_tag="VNR65_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HWJ18538.1"
                     /translation="MLEISTDKVAHIILRAREVDVKTEAWDDENDVDGDDVNSETILE
                     DLPGDATRQELKEFLESLNEDELASLIALAWIGRGTYAPAELDEAVSTAKSEHGGNGI
                     AYLFGLPLLPDYLEDGLDQLGISAEDAEQEIMRPS"
     gene            complement(1055..1744)
                     /locus_tag="VNR65_07430"
     CDS             complement(1055..1744)
                     /locus_tag="VNR65_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWJ18539.1"
                     /translation="MTLSIAPSLAVERNGAALSEPQRDCLFCPRLVDFRQTNRCNYPG
                     WFNSPVPSFGARDASLLIVGLAPGLKGANRTGRPFTGDYAGLLLYETLLAYGFARGTY
                     DQRPDDGLVLTGCRITNAVRCVPPENKPTPDEIAACRGFLAATLGELPKVRAVLALGR
                     IAFETTLRALALKPSQYKFSHGGAVALGGGRTLFGSYHCSRYNTSTGRLTPEMFRDVF
                     DKIAAYLKTAA"
     gene            complement(1760..2452)
                     /locus_tag="VNR65_07435"
     CDS             complement(1760..2452)
                     /locus_tag="VNR65_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWJ18540.1"
                     /translation="MPAKIDRSNHSEVRSQGNRMNFYATERIALFIDGANLYATAKAL
                     GFDIDYKRLLNLFRNKGVLLRALYYTALAEEQEYSSIRPLIDWLDYNGYSMVTKPTKE
                     FTDASGRRKIKGNMDIELAVDAMELADHLDHIVLFSGDGDFRTLVEALQHKGKRVSVV
                     STLTTQPPMVADELRRQADQFIDLADLQKDICRTQRAENGREYQAHIHEQRIHERMSA
                     ATVLRESQVDAD"
     gene            complement(2623..3159)
                     /locus_tag="VNR65_07440"
     CDS             complement(2623..3159)
                     /locus_tag="VNR65_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18541.1"
                     /translation="MQSLIAHKKGTIALFVKSFTAKLWQVTTKLSQKFSCAQDLTRLP
                     DSGAFQEFGCRQPENGMMPEGSHIRKGLQNECPFVGVRVRQYDAHTFYGLLHLPVVNN
                     ILIAHDVEVKWPGFPPLRIFCFTSPRLCLNPLQDFQKSVSGQRCVSRNNRIQEVGLGV
                     AAISARAVQPGRVLDFDT"
     gene            3167..3796
                     /locus_tag="VNR65_07445"
     CDS             3167..3796
                     /locus_tag="VNR65_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ18542.1"
                     /translation="MPKLAPKTQHARREHILDAAERCFIGKGFHHATMDDICREAQVS
                     SGALYTYFVSKEALIIGLCEREKERFGKELAKMTEATDFLAGLRCMADQYCCNEPIEK
                     VRLHVEIGAEAGRNALIGNTVRSIDKAVNESLIELLERERDEGRIKPTAPLEIIVGAM
                     GALGDGLFLRRSLDPHFDPKSIIPAMMTMIEALLAPVPLTSPDEKNRKA"
     gene            3803..>4434
                     /locus_tag="VNR65_07450"
     CDS             3803..>4434
                     /locus_tag="VNR65_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HWJ18543.1"
                     /translation="MFRNRRPLPFLAAVLLGLPLIACLPGVGAFAEEAASTGKLAQKA
                     KPPAVTVTEAKKISFVDNVLVTGSLVPREEVLVGPEIDGYRLTELLAEEGDRVQKGQV
                     LAKLSREILEAQLSQNTASLSKTQAAMDQVRNQITESEATLRQVQDAFNRVKPLRESG
                     VASQATFDDRDAAFRTARARLAAQKEGLKFAEAEQILMRAQRQELEIKLGR"
BASE COUNT         1007 a         1224 c         1239 g          964 t
ORIGIN      
        1 acgcggccgg atcctccctt cgctgaacgg gtcggtgcgc tccgcaaact cctcttcgat
       61 ctcatccagg ctccagggac ggaaatcatg ggcgtcggcg ccgacatcga ggctgtatcc
      121 atgcggctcg tgatcgaggc ggccatggac atgtccgaag aggtgccatg atttgttcca
      181 tgatccgtgc cattcgcgca ttggatagtg gcacaggacg atgcgatgcc cgcgctcatg
      241 gatttcgccg atgtggctca cactcgcaaa cagcggggcg tgatgggcga tcgtctcgtc
      301 gtcatgattg ccggcgatga gatggatccg gccgttcagc tggcgcaaat aggtgtcggc
      361 accgcgcgag ttacggtagc agaaatcgcc aagatggtag attgtgtcgg ctgctccgac
      421 gcgttcgttc caacgagcga caagcgcctc gtccatgtcc gctacgctcg cgaacggtcg
      481 cctgcacatg cgaataatgt tttcgtgccc gaaatgggta tcggatatga agaacaccgg
      541 catgtcaaat cccctcgcaa aacccctcca ggcagattaa taagcaagcc tgcgggatgc
      601 aatgcaagga aagcggctgc gagttatgag gggcgcataa tttcctgttc ggcatcctcg
      661 gcggaaatgc caagctgatc gaggccgtcc tcaagatagt ccggcagcaa aggaagcccg
      721 aaaagataag cgatgccgtt gccgccgtgc tcggacttcg cagtcgagac cgcctcgtcc
      781 aactcggctg gagcataggt tccacggcca atccaagcca atgcgatcag gcttgccagc
      841 tcgtcttcat tgaggctctc aagaaactcc ttcagttcct gccgtgtggc gtccccgggc
      901 aagtcttcaa gaatcgtctc gctgttgaca tcgtcgccat cgacatcgtt ttcgtcgtcc
      961 cacgcctcgg ttttgacgtc gacctcgcga gcccggagaa taatgtgagc aactttatct
     1021 gttgatattt ccagcatggc atcagctcta cttcttaagc agcagttttc aaatacgccg
     1081 ctattttgtc gaagacatcc ctgaacatct ccggtgtcaa cctgcctgta ctcgtgttgt
     1141 aacgggaaca gtgatagctg ccgaagagcg tacggccgcc acccaaggct acagcgccgc
     1201 catgactgaa tttatattgc gacggtttga gagccagcgc gcgcaatgtc gtttcgaatg
     1261 caattcgacc taatgcaaga acggctcgca ccttcggtaa ttccccgagc gtcgccgcga
     1321 ggaagccgcg gcacgcggca atttcatccg gtgtcggctt attctcggga ggaacgcacc
     1381 gcacggcatt cgttatgcga cagcccgtga gcaccagccc gtcgtcggga cgttgatcgt
     1441 aagtcccccg agcgaaccca taggcgagga gcgtttcata gagaagcagt cccgcatagt
     1501 ctccggtgaa aggccgccct gtgcggtttg cccccttaag ccccggggca agtcccacaa
     1561 ttaacaggga cgcatcgcga gcaccgaaag atgggactgg agaattaaac caaccggggt
     1621 aattgcaccg gtttgtttgg cggaaatcca caagccgcgg acagaaaagg cagtcccgct
     1681 gcggctcgct caacgcagct ccattcctct ccaccgctag ggaaggggca attgacaagg
     1741 tcacgccccc cttcgtagac taatcagcgt caacctggct ttcgcgaaga acagtcgcgg
     1801 cgctcatacg ctcatggatc cgttgctcgt ggatatgagc ctgatattcg cggccattct
     1861 cggcgcgctg agtgcgacag atgtctttct gcagatcggc caaatcgata aattggtcgg
     1921 cttggcgacg aagttcatcg gcaaccatgg gtggttgtgt ggtcaaggtt gagacgacgc
     1981 tgacccgttt gcccttgtgc tgaagtgctt ctacgagcgt ccggaaatcg ccatcgccag
     2041 agaataaaac gatgtgatca agatgatcag ccaattccat agcatcgacc gcaagttcaa
     2101 tgtccatatt gcccttgatt ttgcggcggc cggaagcgtc tgtgaactct ttcgtgggct
     2161 tcgttaccat ggaataccca ttgtaatcca gccaatcgat gagtgggcga atcgaggagt
     2221 attcctgttc ttctgcgagg gccgtatagt aaagcgcccg caataagacg cccttatttc
     2281 taaaaagatt caagagacgt ttgtaatcga tatcaaagcc taaagcttta gctgtggcat
     2341 atagatttgc cccgtctatg aaaagggcaa ttcgctcagt tgcgtagaaa ttcatccggt
     2401 taccttggct gcgaacttcg gagtggttgc ttctatctat tttggctggc acggcccaca
     2461 atcccgaatc atgccaaaaa tatagctatg gagtctagaa taccaagaca ttgcgaatga
     2521 tatcaaggtg gcatgataat tattgcgctt ggctctaatc taaatggtgt tttcggaacg
     2581 ccgcatcaat ctatatgtag agcaataaac caactggtcg cgtcaggtat caaaatcaag
     2641 gacgcgtcca ggttgtaccg cacgcgcgct tatggctgca acccccaacc cgacttcctg
     2701 aatgcgattg ttacggctca cacagcgttg cccgcttacg ctcttttgga aatcttgaag
     2761 cggattgagg cacaagcggg gcgatgtgaa gcaaaagatt cgcaacggtg gaaagccagg
     2821 ccacttgacc tcgacatcgt gtgctataag gatgttgttt acaactggaa gatgcaaaag
     2881 cccatagaag gtatgcgcgt cgtactgccg cacccgcacg cccacgaacg ggcattcgtt
     2941 ttgcagcccc tttcggatgt ggctcccttc tggcatcatc ccgttttcgg gttgacggca
     3001 tccgaattcc tgaaacgccc cgctgtccgg caaacgggta agatcctgag cgcagctgaa
     3061 tttctgcgat agtttcgtcg tgacttgcca taacttcgcc gtgaagctct tcacaaaaag
     3121 agcgatcgtt ccctttttat gtgcgatcaa gctttgcaag gcagctatgc cgaaactcgc
     3181 tccaaaaaca cagcacgccc gtcgcgagca catcctcgac gctgctgagc gctgctttat
     3241 cggcaagggc tttcaccacg cgacgatgga cgacatctgc cgcgaggctc aagtcagttc
     3301 aggcgccctt tatacctact tcgtctcaaa ggaagcgttg atcattggct tgtgcgagcg
     3361 agaaaaagaa cgctttggca aagagttggc caaaatgacc gaggcgacag acttccttgc
     3421 cgggcttcgt tgcatggccg accagtattg ctgtaacgag cccatcgaaa aggtccgcct
     3481 ccacgttgag atcggcgcgg aagcgggccg caatgcgctg atcgggaata ccgtccgcag
     3541 catcgacaaa gccgtgaatg aaagcctcat cgagcttctc gaaagggaaa gagatgaagg
     3601 ccggatcaag ccaacggcgc cgctcgagat catcgttggg gcgatgggcg cgcttggaga
     3661 cgggcttttc ctgcgccgca gcctcgatcc gcacttcgat ccaaagtcca tcatccccgc
     3721 catgatgacc atgatagagg cgcttcttgc gcctgtgcct ctcacgagtc cggatgaaaa
     3781 gaacaggaag gcctgattga tcatgtttcg aaaccggaga ccattgccgt tccttgcggc
     3841 cgtgcttttg ggattgccgt tgatcgcctg ccttccgggc gtcggcgctt tcgccgagga
     3901 ggccgcgtca accggtaaac tcgcgcagaa ggcgaaacct cccgccgtga cggtgacgga
     3961 agcgaagaaa atatcttttg tggataatgt gttggtaacc gggtcgctgg tgccgcgcga
     4021 ggaggttctg gtcggccccg agatcgatgg ataccggctg accgagcttt tggccgagga
     4081 aggcgaccgt gttcagaagg gccaggttct ggcgaagctg tcgcgggaga ttctggaagc
     4141 gcagctttcc cagaacaccg cctcgctttc gaaaactcag gccgcgatgg accaggttcg
     4201 caatcagatt actgaaagcg aagcaacgct tcgccaggtg caggatgcct tcaatcgtgt
     4261 gaagccgttg cgagagtctg gtgtggcaag ccaagcgact ttcgacgaca gggatgcagc
     4321 gttccgcacg gctcgggcga gacttgcggc tcagaaagaa ggcctgaaat ttgccgaagc
     4381 cgaacagatc ctgatgcgtg cgcaacgcca ggaactggag atcaagcttg gccg
//
LOCUS       DATMGX010000459         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_13729, whole genome shotgun sequence.
ACCESSION   DATMGX010000459 DATMGX010000000
VERSION     DATMGX010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13729"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VNR65_07455"
     CDS             <1..264
                     /locus_tag="VNR65_07455"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWJ18544.1"
                     /translation="ELEMGRQQMTVQELAVTAHKLVGSDKGILAMDESNSTCNKRFAA
                     VGAPETEEVRRSWRELIVTTPRLGDSISGAILYDETIRQHKKA"
     gene            complement(553..1590)
                     /locus_tag="VNR65_07460"
     CDS             complement(553..1590)
                     /locus_tag="VNR65_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044551230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ18545.1"
                     /translation="MEQIIRIGMDTSKHVFQLHGVNAAEVPVLRKKLRRKEMVAFFEK
                     LAPTVIAIEACGASHHWARLLQSFGHTVKLIPPQLVKPYVKRGKNDAADAEALCEAMS
                     RPTMRFVPVKTAEQQAALMLVSVRDRLIRNRTQLANAIRGHAAEFGLTAAKGMAHLVP
                     LLDRIQADESLPALARELFALQANEYIQMQAQIKEIDAKLTAWHRADECSQRLAKIPG
                     VGPIGAVLLRMKTPDPELFQSGRQFAAWIGLTPKDHSTAGKVRPGGITRAGDEGLRSV
                     LVVGATAVIQQAQRSGKASPWLAALLKRKSPKLAAVALANKIARIAWKLMLTGETYTA
                     KSVATAVAGVA"
     gene            1893..>2137
                     /locus_tag="VNR65_07465"
     CDS             1893..>2137
                     /locus_tag="VNR65_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18546.1"
                     /translation="MDWKAKVELFEQLRREHEFGIGTIAGVAAKFGVHRRVVRRAIAS
                     AVPPPQHYPERAKPKLGALEAFIDKVLDEDCHAPRKQR"
BASE COUNT          385 a          672 c          653 g          427 t
ORIGIN      
        1 gaactggaaa tggggcgcca gcaaatgacc gtgcaagaac tcgctgtcac tgcccacaaa
       61 ctcgtgggca gtgacaaggg aattctcgcg atggacgaga gcaattccac ctgcaacaaa
      121 cggtttgccg cggtgggcgc accggagacg gaggaggtaa ggcgctcgtg gcgcgaattg
      181 atcgtgacca cgccgcggct tggcgacagc atcagcggtg caattctcta cgatgagaca
      241 atccggcagc acaagaaggc ctagacccat atgtggacgg ccccctcctc gcaaggtgtt
      301 ttgcagtgct ttgatcagat cgcttgcgtc catatgtccg gcctgttgat gcgctcgcac
      361 atgaacgctg gccaagatgg tttccgcgac acgggttcca aacaacagag cgacctcata
      421 gagggccatt gggtctgtcg gagtgttccg cgccgtggat cgatcgatcg caccatctgc
      481 tcattatctt gcaagttccg gcatcggctc agcacggttg cagttcaggt gctccccgtg
      541 tctggccaat ctttaggcca cgcctgccac ggcggtggcc acggacttcg cggtataggt
      601 ttcccctgtc agcatcagct tccaggcaat acgtgcaatc ttgttggcca gcgccacggc
      661 ggccaacttc ggcgacttgc gcttgagcag cgcggccagc cagggcgacg cctttccgct
      721 gcgttgagct tgctggatca ccgcggtcgc cccgaccacc agtacgctgc gtaacccctc
      781 gtcgccggcg cgggtgatcc cgccgggcct cactttgcct gcggtcgaat gatccttcgg
      841 tgtcaagccg atccaagccg caaactgccg gcctgattga aatagctcgg gatccggggt
      901 cttcattctc aggagcaccg cgccgatcgg accgacgccg gggatcttgg cgagacgttg
      961 gctgcattcg tcggcccgat gccaagctgt gagcttggca tcgatctctt tgatctgtgc
     1021 ctgcatctgt atatactcgt tggcttggag ggcgaacagc tctcgcgcca aagcgggcag
     1081 gctttcgtcg gcctggatgc ggtcgagcag cggaaccagg tgagccatcc ccttggcggc
     1141 agtgaggccg aactccgctg catggccgcg gatggcgttg gccaactgcg tgcgattgcg
     1201 aatcagccgg tcgcgtacac tcaccagcat caaggcagcc tgctgttcgg ctgtcttcac
     1261 cggcacgaac cgcatcgtcg gtcggctcat cgcctcgcac agcgcttcgg cgtccgccgc
     1321 atcgttcttg ccgcgtttga cgtacggctt gaccagctga ggcgggatca gcttcaccgt
     1381 atgcccgaat gattgcagca gccgcgccca gtgatggctc gcaccgcagg cttcgatcgc
     1441 aatcaccgtc ggtgcaagct tctcgaagaa cgccaccatc tcctttcgcc gcagcttctt
     1501 gcgcagaacc ggcacctcgg cggcattcac cccgtgcagc tggaaaacgt gcttcgacgt
     1561 atccattccg attcggataa tctgttccac ggacggtctc cttttctgag atcaacaaca
     1621 acgacctcat tctggcacgc tcgatgcctc cgggggccgt ccacaccaac aacgaaggcg
     1681 gtgagcatcg gcctgcagcg ggcattagcc tttccgctgt cgcgcgcggc aagcctgcag
     1741 cttgcgtccg acacggcgct ggactgggcc cagcaggaag cggggttcgg catggcaatg
     1801 ctatcgaatt ttggcccagg agtgctggcc aattttggcc caccccccgt ccttgaacct
     1861 acggtcatcc cgatcggatc ggggtgcctt ttgtggactg gaaagccaaa gtggaattgt
     1921 tcgagcagct ccgtcgggag catgagtttg ggatcggcac gattgccggg gtggcggcga
     1981 agttcggcgt gcaccggcgc gtcgtgcgcc gcgcgattgc cagcgccgtt ccgccgccgc
     2041 agcattaccc ggagcgggcc aagccgaagc tgggcgctct cgaggccttc atcgacaagg
     2101 tgcttgacga ggattgtcac gcgccgcgca agcagcg
//
LOCUS       DATMGX010000460         2020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_224942, whole genome shotgun sequence.
ACCESSION   DATMGX010000460 DATMGX010000000
VERSION     DATMGX010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224942"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VNR65_07470"
     CDS             <1..1077
                     /locus_tag="VNR65_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ18547.1"
                     /translation="IMPFALILLSGDTQGPPILGKLAVALAFLLVGAGLHTTQTAGLA
                     LATDLAPEDKRPRVVAFLYVMLLLGMTVSAVLLGRLLEHFSQVRLIEVIQGTALVTMV
                     LNLIALWKQEPRRPRLVLEAEPKPDFSASWHAFMAGGPWRRIFLALGLGTVGFSMQDI
                     LIEPYGGQVLHMTVGQTTALTAFFAMGALFSLALAAHRLDRGADPYRIAAYGVLVGLG
                     ALTAFVFSAPLGEPILFELGVGALGFGSGFFAAGTLTAAMELAKHAQAGLALGAWGAV
                     GASATGGGIALGGALRDIMADLAAQGRFGPALIGADTGYLIVYHVEIALLFATLVAIG
                     PLVRRAGNTSRQTPAHLGLADFPG"
     gene            1099..1875
                     /gene="puhA"
                     /locus_tag="VNR65_07475"
     CDS             1099..1875
                     /gene="puhA"
                     /locus_tag="VNR65_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit H"
                     /protein_id="HWJ18548.1"
                     /translation="MPVGALTTHLDVAQVVLYAFWIFFAILILYLRREDRREGYPLVS
                     EPRGGSETPGWFMIPDKKVFRLFHGGTVTSPQSVPASQKERPIKAVPSSPFGGSPLIP
                     TGNPMLDGVGPGSWVQRSNTPDLTVEGLTRLAPLRVANDFQVDEHDRDPRGFKVIGAD
                     GVVGGTVRDIWVDRSEVTIRYLEVEVKGGKRVLLPITFSKVDQGKGIVKVESILGSQF
                     AAAPTLSSPDRVTLLEEDKVVAYYGGGILYAEPSRQEPLL"
BASE COUNT          345 a          630 c          589 g          456 t
ORIGIN      
        1 atcatgcctt tcgccctcat cctgctttcg ggcgacacgc aggggccgcc gattctcgga
       61 aagctcgcgg ttgccctggc tttcctcctt gtgggagccg gcctccatac aacgcaaacg
      121 gcgggcttgg cactcgcaac ggatttggcg cctgaggaca agcgtcctcg ggtcgtggcg
      181 ttcctttacg tcatgctgct cctcggcatg accgtgagcg cggtcctgct tggccggttg
      241 ctggaacatt tcagccaggt ccgcctcatc gaagtcatcc aaggcactgc tcttgtaacc
      301 atggtgctga acctaatcgc gctctggaaa caggagcccc ggcgtccgag gctggttttg
      361 gaagcagaac cgaagcccga cttttctgcc tcatggcacg cgttcatggc gggcggtccc
      421 tggagaagga ttttcttagc ccttggactt ggcaccgttg gatttagcat gcaggacatt
      481 ctgatcgagc cttatggcgg acaggttctg cacatgacgg tcggccagac aacggccctg
      541 acggcctttt tcgctatggg ggcccttttc tcactcgcgc tggccgccca ccgcctggat
      601 cgtggcgccg atccctacag gatagctgca tacggagtcc tggtggggct tggtgctcta
      661 acggcgttcg ttttctcggc gcctttgggt gagcctatct tgttcgagct cggtgtcggt
      721 gcgcttggtt ttggcagcgg cttcttcgcc gcaggcacgt taacagccgc aatggagctt
      781 gcaaagcatg cgcaggctgg cctcgcgctg ggcgcctggg gcgccgtcgg cgcatcggca
      841 accggcggcg gtatcgcgct tggaggcgcc ctccgcgaca ttatggcaga ccttgcggcg
      901 cagggacgct tcggcccggc cctcatcgga gcagacaccg gttacctcat tgtctatcat
      961 gtcgaaattg ccctgctgtt cgcgacactc gtggccattg gccccctggt tcgaagagcg
     1021 ggtaatacat ccaggcaaac gccagcccac ctcggccttg ccgattttcc aggttaataa
     1081 aactttggag gtatataaat gccagttggc gccttaacaa cacacctcga cgttgcccag
     1141 gtcgtgctct atgcgttctg gatctttttc gcgatcctca tcctgtatct ccgccgcgaa
     1201 gaccggcgcg agggctatcc cctggtttcc gaaccgcgcg gcggcagcga aaccccaggt
     1261 tggttcatga tcccggacaa gaaggttttc cggctgttcc atggcggcac agtgacctct
     1321 ccgcagtctg tacctgcgtc ccaaaaagag cgcccgatca aggctgtgcc ctcaagcccc
     1381 ttcggcggat cgccgttgat cccgaccggc aatcccatgc tggatggcgt cggcccgggc
     1441 tcctgggttc agcgctcaaa caccccggac ctcaccgttg agggcttgac ccggctcgcc
     1501 ccacttcgcg tcgcaaacga tttccaggtc gacgagcatg atcgcgatcc gcgcgggttc
     1561 aaggtcatcg gggccgatgg cgttgtcggc ggcacggttc gcgatatctg ggtcgatcgc
     1621 tcggaagtga ccatccgcta cctcgaagtc gaggtcaaag gcggcaagcg cgttctcctt
     1681 ccgatcactt tttccaaggt cgatcagggt aagggaatcg ttaaagtcga atccatcctt
     1741 ggcagccagt tcgccgccgc tccaacactt tcgagcccgg atcgcgtgac gctccttgag
     1801 gaggataagg tcgtggccta ttatggcggc ggcattctct acgcagagcc ttcccgccag
     1861 gagccccttt tatgagtgaa tacgacttcg agccggtgcg gggcctcccg ttccggcctt
     1921 ccgaagacga aaagatatta tggcagggat cgccaaattg gcgatcctta gctgttcatc
     1981 tttgccatgt ccgctccatc acgatctact tcacagtgct
//
LOCUS       DATMGX010000461         3434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_444936, whole genome shotgun sequence.
ACCESSION   DATMGX010000461 DATMGX010000000
VERSION     DATMGX010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444936"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..234)
                     /locus_tag="VNR65_07480"
     CDS             complement(64..234)
                     /locus_tag="VNR65_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18549.1"
                     /translation="MRTESKNWRKTTFFEPNLSEGKPLRATEEPNQASTYSSITTMPS
                     AGEASQHRREGW"
     gene            complement(294..1727)
                     /locus_tag="VNR65_07485"
     CDS             complement(294..1727)
                     /locus_tag="VNR65_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HWJ18550.1"
                     /translation="MTQCIPTLFGFQDLGSREVVAAFDGGKVSSDAGGLLLREVEEKF
                     RFVEQFARCFTDYREADLVEHTVLELIKQRVFGICLGYEDLNDHDQLRLDPLLAVLVG
                     KTDPTGDNRVRARDQGKPLAGKSTLNRLELTAVGDDDESRYKKIVARMSQVENFLVDV
                     FVQQHSAAPPRIVLDLDATDDPIHGHQLGKFFHGYYDCDCYLPLYIFCGDHPLCAALR
                     PSDIDASAGSLVYIQRIVARIREKWPNVKIVLRGDSGFCRDYLMTWCEANNVDFLFGL
                     AKNKRLLRVIGQEMQEAKVQFEQTQKASRVFKDFEYQTRRSWKRARRVVGKAEHLKKG
                     ANPRFIVTSLSSEDFDAKTLYEKEYCARGEMENRIKEQQLMMFADRTSCETLRANQVR
                     LYMSTVAYIVMRALREFGLKDTEMAQAQCSTIRVKLLKLGAVIRVTVRRVVVSLSEAF
                     AFREVFMKVWRNLRDLVTPLPIVAPTG"
     gene            complement(1913..3301)
                     /locus_tag="VNR65_07490"
     CDS             complement(1913..3301)
                     /locus_tag="VNR65_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HWJ18551.1"
                     /translation="MDVLGNVPVDVALDREFDEAFELLNGLVDWRQADELMPLGPAAI
                     YTASVVLWMLVYQRMHAGASLQAAVKHLIEAPPEHCPQNKRLREKTISGNTAAFSQAR
                     QRLTPEVTQWFASRVCDSLIAASPPSWQGRRVYLIDGTTITLAPEPELQKAFPPASNQ
                     HGEGVWPVALLLVAHELASGCALLPEIGPMYGPLAIAETALVPACVQRLPSASIIMGD
                     ANFGIFAVAHAAAQAGHDFLLRMTAPRFHALARKAQLTKQGAGWKTYSHQWTPSAKER
                     KTHPGLPADAHLAVRLHEIEIHETLTLWLVSGLPDSAVKLSGLYRKREYVETDLRNLK
                     VVLDTEHIRARSLKMFHKELQTSMVAYNLVVQFRRQAADLARLTPRQLSFTGVWTTFR
                     QFLMNRLHTDPMTWRESYRRALSYAMRDKLPHRPGRSYPRTAYPKRHKSSQFAKREKP
                     QKTDNPDDESTK"
BASE COUNT          678 a         1016 c         1010 g          730 t
ORIGIN      
        1 cgccgcatca catccacgcg aagtgaaaat gccatgaaca tccgcttcct tagcccgtaa
       61 ttctcaccag ccctcgcgcc ggtgctgcga tgcctctccg gccgatggca tggtggtgat
      121 cgaggagtag gtggatgcct gattgggctc ctcggtcgcg cgcagcggct tgccttcgct
      181 caaattcggc tcaaaaaagg tcgtttttcg ccagtttttc gactcggtgc gcacctcgga
      241 cgccttcgcg gcggcgaatt tttccccggc gccgattacc cgccatcagg gagctatcct
      301 gttggagcga cgatcggcaa cggcgtcacc aagtcgcgca ggttccgcca aaccttcatg
      361 aacacctctc gaaacgcgaa tgcttccgag agcgacacca ccacccggcg aaccgtcact
      421 cggatcaccg cgccgagttt cagcaacttc acgcggatcg tgctgcattg ggcctgagcc
      481 atctccgtgt ccttgaggcc gaactcgcgc agcgcccgca tcacgatgta cgcgaccgtc
      541 gacatgtaca gtcgcacttg gttggcccgc aacgtctcgc aactggtgcg gtcggcgaac
      601 atcatcaact gctgttcctt gattcggttc tccatctcgc cgcgagcgca gtactccttc
      661 tcataaagcg tcttggcgtc gaagtcctcc gacgacagcg aagtcacgat gaaacgcggg
      721 ttggcgccct tcttcaaatg ttcggccttg cccaccacgc gccgcgcccg tttccagctt
      781 ctccgcgttt ggtactcgaa gtccttgaac acgcgagacg ctttctgagt ctgctcgaac
      841 tgaaccttgg cctcttgcat ttcctggcca atgacgcgta gcagacgctt gtttttcgcc
      901 agtccaaata ggaaatcgac gttgttcgcc tcgcaccagg tcatcagata atcgcggcaa
      961 aaaccgctgt cgccacgcag gacgatcttc acgttgggcc acttttctcg gatgcgcgcc
     1021 acgatccgct gaatgtacac cagcgaaccg gccgaggcgt cgatgtcgct gggccgcagc
     1081 gcggcgcaca gcggatggtc gccgcagaag atgtacagcg gcaggtaaca gtcgcagtcg
     1141 tagtagccgt gaaagaactt gccgagttga tggccatgaa tgggatcgtc cgtggcatcg
     1201 agatcgagaa cgatgcgcgg cggcgccgcc gaatgctgct gcacgaaaac atcgacgaga
     1261 aagttttcga cctggctcat gcgcgcgacg atcttcttgt aacggctctc gtcgtcgtcg
     1321 ccgaccgccg tgagttcgag tcggttgagc gtgctcttgc cggccaaggg cttgccttgg
     1381 tcgcgcgctc gaactcgatt gtcgccggtg ggatcggtct tgcccaccag cacggccaac
     1441 agcggatcca gtcgcagttg atcgtggtcg ttgagatctt cgtagcccag acagattccg
     1501 aagacgcgct gcttgatgag ttcgagcacc gtatgctcga cgaggtcggc ttcgcgataa
     1561 tcggtgaagc atcgcgcgaa ctgttccacg aagcgaaact tctcctcgac ttcgcggagc
     1621 aacaagccac ccgcatccga actcaccttg ccgccgtcga aagcggcgac cacttcgcgc
     1681 gaaccgaggt cttggaaacc gaacagagtg ggaatacact gtgtcataac ccgaccgctc
     1741 ctttgttgag tcgtaagatg ttttcctaac aatacttacg acccaaagag cggtcgggtt
     1801 tcatcggtta gtgagaaatt cgggttagcc ctgaaagggc gacagctttc tgccgccctt
     1861 tcagggcttc cggttgcgtg caatacgacc aggggcttgc gcccaatggc acttactttg
     1921 tcgattcgtc gtcggggttg tcggttttct gtggtttttc cctttttgcg aactgcgacg
     1981 acttatgtcg tttggggtag gcggttcgag ggtagctgcg gcctggtcga tgcgggagtt
     2041 tgtcgcgcat ggcataagac agtgctcggc gataggactc gcgccaggtc atcgggtcgg
     2101 tgtgaagccg gttcatcagg aattgtcgaa acgtcgtcca gactccggtg aagctgagtt
     2161 gacgaggcgt cagccgggct agatcggccg cttgccgacg gaactgcacc accaaattat
     2221 aagccaccat cgacgtctgc agttctttgt gaaacatctt caggctccgg gcccggatgt
     2281 gctccgtgtc cagcacgact ttgagattgc gcagatccgt ctcgacgtat tcgcgtttgc
     2341 gatacagccc ggacaatttg acggcggagt cggggagtcc gctgaccagc cagagggtta
     2401 gcgtttcgtg gatttcgatt tcatgcaagc gcacggcgag atgagcatcc gcgggcaggc
     2461 cgggatgcgt tttgcgttcc ttggcgctgg gggtccattg atgcgaatag gttttccatc
     2521 cggccccctg ctttgtcaac tgagcctttc gagccaaagc gtggaatcgc ggagcagtca
     2581 tgcgcagcag aaagtcgtgt cccgcttgtg ctgccgcatg agcgaccgcg aagatgccga
     2641 agttcgcgtc gcccatgatg atcgaggcac tcggcagtcg ctgcacgcac gccggcacca
     2701 gcgctgtctc agcgatggca aggggaccat acatcggccc gatctcggga agcaacgcgc
     2761 agccactggc gagttcgtgc gcgaccagca gcagagccac cggccaaact ccctcgccgt
     2821 gctgattgga agcgggtgga aaggctttct gcaattccgg ctcaggagcc agcgtaattg
     2881 ttgtgccgtc gatcaaatag acacgccgac cctgccaaga tggcggcgag gcggcgatca
     2941 gcgaatcgca gacccgactg gcgaaccact gggtgacttc gggagtgagc cgctgacggg
     3001 cctgactgaa ggcggcagtg ttgcccgaaa tggtcttttc tcgaagccgt ttgttctgcg
     3061 gacagtgctc cggcggagct tcaatcagat gtttgaccgc tgcttggaga gaggctcccg
     3121 cgtgcatgcg ctgatagacc agcatccaca acacgacgct ggcggtataa atcgccgccg
     3181 gtcccagggg catcaactcg tcggcctgcc gccagtcgac cagaccgttc aacagctcga
     3241 acgcctcgtc aaactcgcga tccaaagcca catccacggg aacattgccg agaacatcca
     3301 tgacaaaatg actcctgaaa gagaagtcat ctttggggcg caaatcttat gccaagttct
     3361 gcggctgagg tggatttgat aaagtaagtg ccattgggct agcgcccctg gctagtgcgc
     3421 ctgtgaaggt agtg
//
LOCUS       DATMGX010000462         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_443684, whole genome shotgun sequence.
ACCESSION   DATMGX010000462 DATMGX010000000
VERSION     DATMGX010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443684"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VNR65_07495"
     CDS             complement(<1..491)
                     /locus_tag="VNR65_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18552.1"
                     /translation="MRHVARSVTLKRDSSWIPLIAALLMISIDAAAISNAAPISQAPT
                     GLSPDINVENWYWGPGNRWSYKNTRRIFPSADIYRGDGPVAQLAYAPRTIDNITFVHP
                     TTKAVTTVAQMFAQTDTDAFLVMKDGKILTERYFNGMNPADTHLLMSVTKSVVGALAG
                     III"
     gene            complement(935..2092)
                     /locus_tag="VNR65_07500"
     CDS             complement(935..2092)
                     /locus_tag="VNR65_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18553.1"
                     /translation="MNNLIYEQWHFARPKLVKSYLRLLLDGPGDPIALQSPRRWGKTT
                     FLQTEIMPAAQAAGFLPVYIDVWQNRDDALSAINYGLQEAIDDLDVPPTTVGKRLKTN
                     VKKLGVASLLLELGEEPTRTRPDSAFLLVDWLLKTLVRKAKRPVLLLFDEIQELAVAK
                     SSEAVVSALRSAITKSRQSVRVIFTGSNQDRLSELFSRSRAALYEGASILPFPALGLD
                     FLEFVVALCDKRFRRRIPVSDLTLAFERFQYQPRPLIDLVFLFVSGEKTSFKAVLNER
                     IERLLASDQYQSVLDQMSPLQKQICRRLAAGGDVSSTGAQRVYAEALGTEGISPGSIS
                     RALRSLIDLHVLTKPAGARGGYIFDDPMFREWLGRVTWLRLSTSVRVSTSR"
BASE COUNT          470 a          631 c          616 g          445 t
ORIGIN      
        1 tcgattatga ttcccgcaag cgcgccgacc accgatttgg tcaccgacat gagaaggtgg
       61 gtgtcggcgg ggttcatgcc gttgaagtag cgttcggtaa gaatcttgcc gtccttcatg
      121 accaagaatg cgtcggtgtc cgtctgcgcg aacatctgtg ccactgtggt caccgctttg
      181 gtggtcggat ggacgaaggt aatattgtcg atagtgcgtg gcgcataggc cagctgcgca
      241 accgggccgt ctccgcgata gatgtcggcg ctcggaaaaa tccgacgtgt atttttgtag
      301 gaccaccgat tccccggccc ccaataccaa ttctcgacgt tgatgtctgg agacagaccg
      361 gtcggcgcct gcgaaatcgg tgcggcgttt gagatagcgg ccgcgtcgat cgaaatcatc
      421 agcaacgcgg cgatgagtgg tatccaggag ctatctcttt tgagcgtgac gctacgagcg
      481 acgtgcctca ttttcttatg ttcctccatt cgccgattgc acgagtttct gcccaatgtc
      541 ttagtaattc gaacgcgctc ttaggcatcg agataccgca ctcagaaggt gattgcgcat
      601 caacgggcgc gactcacaaa cacccaaagt agtccagaag gcggtattgc ctgtcttcct
      661 ttgaggttac cacgccgttc tcgccgtcaa ggctgttctc cgtcgggccg gcccattcac
      721 gcatccatgc gttcggccgg cgacggcgac ccagaggcgc gactccgggt cccctcccgg
      781 attctcgggc cggagcccct cgaacccgtt gcgacgtagc tgtcgcaaat tgcactcaac
      841 tgcggctgcg ttaggcacgc ccacaccgtc aaaccctctg ggcgatagcc ggaccgcgaa
      901 gcggcgtctg cctgcgccaa acggacaaaa tgaatcagcg actcgtcgaa acccggacgc
      961 tagtcgaaag acgcagccaa gtgactcgac ccagccactc gcggaacatc gggtcatcga
     1021 agatgtaacc gccgcgcgcc ccggccggtt tcgtgaggac gtgcaggtct atcaacgatc
     1081 gcagcgcccg actgatcgag ccgggcgata tcccttcagt accgagggcc tccgcatata
     1141 cccgttgcgc gcccgtggag gacacgtcgc cgccagcggc aagacgccga caaatctgct
     1201 tttgtaaggg gctcatctgg tccaagacgg actgatattg gtctgaggcc aatagacgtt
     1261 caatacgctc attgaggacc gccttaaacg aagttttctc accggagaca aatagaaaga
     1321 ccaggtcaat gagtgggcgc ggctggtatt ggaatcgttc aaatgccagg gtgagatcgc
     1381 tgacaggaat gcgccgccga aatcgcttat cgcagagtgc cactacgaat tccaagaaat
     1441 ccagcccgag cgccgggaag ggcaggatgc tcgccccctc gtacaacgcc gctctggatc
     1501 gggaaaatag ttcgctcaat cggtcctggt tggaaccggt gaagatcacg cgcaccgact
     1561 gacgactctt ggtgatggcc gatcgaagtg ctgaaacgac agcctcgcta ctcttggcta
     1621 ccgcaagctc ttggatctca tcgaagagca gcagcaccgg tctcttcgcc ttgcgcacca
     1681 aggtcttcaa cagccagtcg accaaaagaa aggccgagtc gggtcgcgtt cgtgttggct
     1741 cctcgcccaa ctcgagcaag agcgaggcga cgccgagctt tttgacgttc gtctttaagc
     1801 gcttgcccac cgtagttggc ggcacatcga gatcatcaat cgcttcttga aggccgtagt
     1861 tgatcgccga gagcgcgtcg tcccgattct gccaaacgtc aatatagact ggcaggaaac
     1921 ctgctgcttg ggcggcgggc atgatttcgg tttggagaaa ggtcgtcttg ccccagcgcc
     1981 gcggcgattg aagcgcgatc ggatcccccg gaccatcgag tagcagccta aggtaggact
     2041 tcaccaactt cgggcgcgcg aagtgccatt gttcgtatat caaattattc attacggcag
     2101 attaccgaaa tgcgcgtaat tacgcaattc gcggcaatag tgcgtaatca cccgtcaaga
     2161 ga
//
LOCUS       DATMGX010000463         2368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_393200, whole genome shotgun sequence.
ACCESSION   DATMGX010000463 DATMGX010000000
VERSION     DATMGX010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393200"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VNR65_07505"
     CDS             complement(<1..752)
                     /locus_tag="VNR65_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWJ18554.1"
                     /translation="MSAAALEIRDELTSLERVGLALQYKKPDRVPAAPLVCGASCRVL
                     GYSYDRWSQDPKIAVASLLAAQELIGFDGFLTLVDLSVEAEDFGQEVIFPERSTALPN
                     FDNPFIATAEDYNKVRPVDPRKSRRMSAVIEIVRGLVEAKGKEVPTMGFVYGPLGVLS
                     QIRGHAELFKDIIRHPDRVLEAVHTINDVLVDYALAQVQAGAHAIVLDPLYSSASILK
                     KSTWVKFEAEIFKSVADAVRDAGVPVIVHNCGG"
     gene            complement(749..1411)
                     /locus_tag="VNR65_07510"
     CDS             complement(749..1411)
                     /locus_tag="VNR65_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HWJ18555.1"
                     /translation="MPDGRLKLYEEIANAVLELDEECTKALSHEVLKRGYDAWEAVEQ
                     GLILGVNRAGKLFDEEEYFVPELLIAADAMYAGLDILRPHIKEQFTGHTRKVVIGVVQ
                     GDTHDIGKNLVRIMLEVAGFEVHDLGRDVPPRKFVDRAVEVKADIIALSTLMTTTMER
                     MGEVIGILEDENLRNRFKVIVGGSPLSQQFADRIGADGYAPKAAQAVQLAKRLSGLVN
                     AG"
     gene            1471..1650
                     /locus_tag="VNR65_07515"
     CDS             1471..1650
                     /locus_tag="VNR65_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18556.1"
                     /translation="MTPIAGWNAGAAACATDGVKLYPSIRQGGDCIVEASFQTITIAV
                     AATIAAAAIPRQRQS"
     gene            complement(2057..2299)
                     /locus_tag="VNR65_07520"
     CDS             complement(2057..2299)
                     /locus_tag="VNR65_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18557.1"
                     /translation="MGEGELMSDAYVIELQGNAVGIIVRKHKNESNYRFLSALHSFNS
                     LEGQEFPGPLQAEIAARKVLKESPHALQKGRFKQRY"
BASE COUNT          525 a          716 c          641 g          486 t
ORIGIN      
        1 ccaccgcaat tgtggacgat gaccggaacg ccagcgtcgc gaacggcgtc cgcgacggat
       61 ttgaagatct ccgcctcaaa cttcacccag gtggatttct tcaagatgct ggccgaggaa
      121 tagagcggat cgagcacgat ggcgtgcgct cccgcctgca cctgcgcgag cgcatagtcg
      181 acgagaacgt cattgatggt gtggacggcc tcaagcaccc gatcggggtg gcggataatg
      241 tccttgaaca attccgcgtg cccacggatt tgcgacagga cacccagggg gccatagacg
      301 aagcccatgg tgggcacttc cttgcccttc gcctccacca atccgcgtac gatctcgatc
      361 acggccgaca tgcgccgcga tttgcgagga tcgaccggcc tgaccttgtt gtaatcctcg
      421 gcggtcgcga tgaacgggtt gtcgaagttc ggaagcgcgg tgctgcgctc cggaaagatc
      481 acctcctggc cgaaatcctc cgcttcaacc gataagtcga cgagcgtcag gaagccatcg
      541 aagccgatca gttcctgcgc cgccaacagg ctcgcgacag caatcttcgg gtcttgcgac
      601 cagcgatcgt aggaatagcc cagcacccgg cacgacgcac cgcaaacaag cggggccgcg
      661 gggacccggt cgggcttttt gtactgcaag gcgaggccga cccgctccag cgaggtcaac
      721 tcatcgcgaa tttccaaagc cgcggcgctc atcctgcatt caccaatccg ctaaggcgct
      781 tggccagttg cacagcctgg gcggctttcg gcgcgtaacc atccgcgccg atacggtcag
      841 cgaattgctg cgaaagcggc gagccgccga cgatcacctt gaagcggttg cggaggtttt
      901 cgtcctcgag gatgccgatg acctcgccca tccgttccat ggtggtggtc atcagggtgg
      961 acaaggcgat gatgtccgcc ttcacctcga cagcgcggtc gacgaacttc ctgggcggga
     1021 cgtcgcggcc aaggtcgtgg acctcgaagc cggcaacctc cagcatgatg cgaacgaggt
     1081 tcttgccgat atcatgggtg tcgccctgga cgacgccgat gacgactttc cgggtgtgcc
     1141 ccgtgaattg ctccttgatg tgcgggcgca agatgtcgag cccggcgtac atggcgtccg
     1201 cggcgatcag gagctcaggg acgaaatatt cctcttcgtc gaacagcttg ccggcgcggt
     1261 tgacgcccag aatgagcccc tgttcaacag cttcccaggc gtcgtagcct cttttgagaa
     1321 cctcgtgcga taaagccttc gtgcattcct catccagttc gaggactgca ttcgctatct
     1381 cttcatacaa tttcagccgg ccgtcgggca tttccccctc ctttgcaatc agtcccaggc
     1441 aaagcaagcc acgcccccga cgaggcgaat gtgaccccga tcgcaggatg gaatgcaggc
     1501 gccgcagcct gcgcaactga tggagtcaag ctctacccct ccatcaggca aggcggcgat
     1561 tgcatcgttg aagcaagctt ccagacaatc acgatcgcag tcgccgcaac tatcgccgcg
     1621 gccgctatcc ctcggcagcg ccagagctga agcttgttcc ctcgccatca accctcccta
     1681 gccgtttgca accacaatcc ttaataccgt agataaagat tatggaatta gtcaatataa
     1741 taataacttt caggattgat ctagatcaga gcaagaataa aactgttctc tagctcgcgc
     1801 caccaaaaac cgtttgcgca tcagcaagga aaacgcaaat tcactcttca aatgttatat
     1861 tttaaaggaa aagaagttct ggcacttgca cagtctgtgc ccccgacccg accgggccaa
     1921 gctcccggga ggaggagctt ggcccgacta agcgccacag ttcagatcat ggccccgatc
     1981 tgaactgtgg ctcggatgcc ccgcacagac cagcaatcgt gggggcaact caactcatca
     2041 aaaagagcaa tacatattag tatctttgct tgaatctccc cttttgcaac gcgtgcgggc
     2101 tttctttcaa gaccttcctc gccgcaattt ctgcttgaag cggacctgga aattcctggc
     2161 cttcaaggct gttgaaggaa tgcagcgcgg acagaaacct gtaattgctc tcatttttgt
     2221 gcttacgcac aataatccca accgcgtttc cctgcagttc gatgacatag gcgtcagaca
     2281 taagttcacc ctcgcccatt gaatggcgtt gttgaaatcg ttaatgttat gaggaagtga
     2341 tcctaatcgc ggcgacaaca acgtgggg
//
LOCUS       DATMGX010000464         4577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_34837, whole genome shotgun sequence.
ACCESSION   DATMGX010000464 DATMGX010000000
VERSION     DATMGX010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34837"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /gene="ppsA"
                     /locus_tag="VNR65_07525"
     CDS             <1..1499
                     /gene="ppsA"
                     /locus_tag="VNR65_07525"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HWJ18558.1"
                     /translation="AKAGHPMPMDIEWAKDGEDGRLYIIEARPETVASERPAETFEIY
                     ALQGKGALLASGRAVGEKIAVGKVRVVSSAEDLAAFQPGEVLVCDATTPDWDPVMKTA
                     AAIVTNRGTRTSHAAIVARELGVPAVVGTASATKKLKPGVAVTVSCAEGGTGRVYEGE
                     IPFGMTRVAAASVPRPRTEIKVNLSDPALAFKAAMLPNDGVGLARMEFIINEHIGVHP
                     MALAEPERVTSQADRTIIAKLTAGYERPADFFIERLSEGLGTIAAAFYPKPVNVRFSD
                     FKTNEYARLIGGADFEPKEENPMIGFRGASRYTHPAYAAGFALECAALKRVRDQMGLT
                     NLFVMVPFCRRVEEARRVISAMTANGLRQGEKGLKIYVMCEVPNNVISIDAFSEWFDG
                     FSIGSNDLTQLTLGVDRNSDIVAFEFDERDAGMLAMFRLAITGAKRNGRRVGICGEAP
                     ANYPEIARFLTELGIDSISVNPASIMRTMRVMRQAEDLAKRGPGDAAT"
     gene            1696..2274
                     /locus_tag="VNR65_07530"
     CDS             1696..2274
                     /locus_tag="VNR65_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWJ18559.1"
                     /translation="MFQIRIHGRSGQGNVTAAAILSVAAFFEGRHAQAFPSFGVEHLG
                     APVVAFCRIADKEIRLREPIMEPDCLIVHEPALIRAVDAFAGLRPGGYLLVNTDKAFS
                     EAHIRTVADSLPKGHAVAVPATDLARRHGGARPNAALLGSFAALTGLVQLDSVKRAIG
                     EAFPGELGAANIASAVEGYEFVMTQQHAASAA"
     gene            2285..3550
                     /locus_tag="VNR65_07535"
     CDS             2285..3550
                     /locus_tag="VNR65_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18560.1"
                     /translation="MLKQIEGSRAMAEAIALCRPEVICAYPITPQTHLVEGLGEMVKG
                     GRLANCEFINVESDFSALSVAIGASAAGARAYTATSSQGLLLMAEAVYNASGLGLPIV
                     MTLVNRSIGAPANIWNDHSDAMSMRDAGWVQLFAETNQEAVDLHIQAFRLAEELSIPV
                     MVCVDGFILTHAVERVDIPNQAMVDAFLPPYDPVQVLDPAEPVTMGAMVGPDAFTEVR
                     FLAHYKQTKALRFITQFADSFAKVFGRESGGLLRGYRTEDAQMVVIAMGSVCGTIKDT
                     IDEMRDDGIPIGLVTLVSYRPFPIDALQKILESAREVLVIDKSVSLGMGGPLANNVGV
                     AVKSLPRRPRLHSVVAGLGGRPITRQSLHRLFRQAATEPWEGAHFLDMNDRVVGREIH
                     RAGKLRRSGPTAEAILRYLAENAPDRAAE"
     gene            3561..>4577
                     /locus_tag="VNR65_07540"
     CDS             3561..>4577
                     /locus_tag="VNR65_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWJ18561.1"
                     /translation="MNDRPRSLAPAEASEKLKFYQKGTFTVGNRLVSEEERSVQATQE
                     RSNAITCGHRACPGCGEALGARYALDAAMRATGDDLIAVCATGCLEVFSSRYPETSWQ
                     MAWLHSLFGNAPAVATGIAAAMRVKKRATRVIAQGGDGGTNDIGFQCLSGLFERNDDV
                     LYVCYDNEGYMTTGVQRSSATPPAAGTATTPAVGPSPGNVFGTGKSMPKIALAHGIPY
                     VATASVADLHDLERKVTKAMSIHGARYIQIHVPCPFGWGAASHDTIRLARLAIECGFY
                     PIFEAEHGKVTHVRKIRRKVPVQYYLMLQRRFAHLFRDGKPDPSVARLQAICDANIAD
                     YGLLD"
BASE COUNT          883 a         1398 c         1405 g          891 t
ORIGIN      
        1 cggccaaggc aggccatccg atgccgatgg acatcgagtg ggcgaaggac ggcgaggatg
       61 gccgtcttta tataatagag gcgcgtcctg agacggtcgc ctcggagcgt ccggccgaaa
      121 cgttcgagat ttacgcgctg caggggaaag gggcgctcct cgcgagcggc cgggccgtcg
      181 gcgagaagat cgctgtcggg aaagtgcgcg tcgtctccag cgccgaggat ctcgcggcgt
      241 tccaaccagg cgaagtgctt gtctgcgatg cgacgacccc cgactgggat ccggtcatga
      301 agaccgccgc agccattgtc accaatcgcg gcacccgcac ctctcacgcc gccattgtcg
      361 cgcgggagct tggcgtaccc gccgtcgttg gtaccgcatc ggcaacaaag aagctcaagc
      421 ccggtgttgc agtcacggtg tcttgcgccg agggcggaac gggtcgtgtt tatgaaggcg
      481 aaattccctt cggaatgacg cgcgtcgccg cagcctccgt tccacggccg cgaaccgaga
      541 tcaaggtaaa cctcagcgat cccgctcttg ctttcaaagc cgcgatgctg ccaaacgatg
      601 gcgtcgggct tgcgcgtatg gaattcatca tcaacgagca catcggggtc catcccatgg
      661 cgcttgctga gcctgagcgg gtcacctcgc aggcagaccg caccatcatc gcaaagctca
      721 ccgcgggcta cgagcgccct gcggatttct ttattgaaag gctttcggaa ggcttgggca
      781 ccatcgcggc cgccttctat ccaaagcccg tgaatgtccg gttttccgac ttcaaaacga
      841 acgaatacgc gaggctgatc ggcggagccg acttcgagcc caaggaagaa aacccgatga
      901 tcgggttccg aggcgcgtca cgctataccc atccggccta cgccgcgggc ttcgcgctcg
      961 aatgcgcggc gctgaagcgc gttcgcgacc agatggggct gaccaatctt tttgtcatgg
     1021 tgccgttctg ccggcgcgtc gaggaggcgc gccgcgtgat ttcggccatg acggccaacg
     1081 gcctgagaca gggcgaaaaa ggccttaaga tttatgtcat gtgcgaggtt ccgaacaatg
     1141 tcatcagcat cgacgccttc tccgagtggt tcgatggctt ctcgatcggg tcgaacgatc
     1201 tcacccagct gacgcttggc gtcgaccgca actcggacat cgtcgccttc gagtttgatg
     1261 agcgcgatgc aggcatgctt gccatgttcc ggcttgccat cacgggcgcg aaacggaatg
     1321 gacgaagggt gggaatttgc ggcgaagcgc cggcaaatta tcccgagatc gcgcgcttcc
     1381 tgacggagct tggtatcgac tcgatcagcg tcaatccggc aagcatcatg cgaacgatga
     1441 gggtgatgcg ccaggcggaa gatcttgcaa aacgcggccc cggtgacgct gccacttgat
     1501 gattgcgagc cgcttcgtct tgacccgtta ttttttgacg aggatcaaat atataccccc
     1561 gtggcacatg taatatgtaa gactaatgct ggggaaaaag ataaaaaaat aggctaagct
     1621 ctgggtcggg cctaaccgat aacggaggaa agcattcccc atcccttggc accttcgaag
     1681 agttggggga gaaaaatgtt ccaaatccgc atccatggcc ggagcgggca gggtaatgta
     1741 acggctgcgg caattctttc ggtcgcggca tttttcgaag gacgccacgc acaggccttc
     1801 ccgagtttcg gggtggagca tctgggcgcc cccgtggtgg cattctgccg gattgcggac
     1861 aaggaaatcc ggcttcgcga gccgatcatg gagcccgatt gcctcatcgt gcacgagcca
     1921 gccctgatca gggcggtcga tgccttcgcg ggtttgcggc cgggcggcta tcttcttgtc
     1981 aacaccgata aggcattttc tgaggctcat attcggacag tcgcggatag tctgccgaaa
     2041 gggcacgccg ttgccgttcc ggcgactgac ctcgctcgaa ggcatggagg cgcacggccg
     2101 aatgcggcct tgctcggctc atttgctgcc ctgacggggc tcgttcagtt ggacagcgtc
     2161 aagcgcgcga tcggggaggc tttccccggc gagctcggcg cggccaacat cgcaagcgcc
     2221 gtggaaggct acgagtttgt gatgacccaa caacacgccg ccagcgcagc ctgaggagca
     2281 agcgatgctg aagcaaatcg aagggtcgcg cgctatggcc gaagcaatcg ccttgtgccg
     2341 gccggaagtg atttgcgcct accccatcac gccgcagacc catcttgtcg agggcctcgg
     2401 cgagatggtg aagggcggca gactcgcaaa ttgcgaattc atcaatgtcg agagcgattt
     2461 ctcagcgctt tccgtcgcca ttggcgcctc tgctgcgggc gcgcgcgcct acaccgcgac
     2521 gtcgtcgcag ggcctccttt taatggcgga ggctgtttac aacgcttccg gcctcggtct
     2581 gcccatcgtg atgacgctcg taaacaggtc catcggcgcg ccagccaata tctggaatga
     2641 tcattccgac gcgatgtcca tgcgcgatgc aggctgggtt cagctcttcg ccgagacgaa
     2701 ccaggaagcc gtcgacctgc acattcaggc cttcaggctc gccgaggagc tttcaatacc
     2761 ggtcatggtc tgtgtcgacg gtttcatcct gacgcatgcg gtcgagcgcg tggatattcc
     2821 aaatcaggca atggtcgacg cctttctgcc gccctacgat cccgtccagg tgcttgatcc
     2881 ggcggagccg gttaccatgg gcgccatggt cgggccggac gccttcacag aagtccgctt
     2941 tcttgcgcat tacaagcaaa cgaaggcctt gcggtttatt acgcagtttg ccgattcctt
     3001 tgcgaaagtg ttcgggcgcg agtctggcgg tctcttgcgc ggctatcgca ccgaggacgc
     3061 ccagatggtc gtgatcgcga tgggctcggt ctgcggcacc atcaaggata cgatcgatga
     3121 aatgcgcgac gacggcattc cgataggcct tgtcacgctt gtctcctatc ggccattccc
     3181 gatcgacgcc ttgcagaaga tcctcgaaag cgctcgggag gtcttggtga tcgacaagag
     3241 cgtgtccctt ggcatgggcg gcccgctcgc aaacaatgtc ggcgtggcgg tcaaaagcct
     3301 gccgcgccgg ccacgcctgc attcggtggt ggcgggcctt ggcggccgtc ctatcacccg
     3361 acagtccttg caccgcctct tccggcaagc cgcgaccgag ccctgggagg gagcgcattt
     3421 cctcgatatg aacgaccgcg tcgtggggcg cgaaatccat cgcgctggca agctgcgccg
     3481 ctccggtccc accgctgaag ccatcctgcg ctaccttgcg gagaacgctc ccgatagggc
     3541 ggctgaatga gaggcaagac atgaacgacc gcccccgatc gctcgcgccg gccgaagcct
     3601 cagagaagct caagttctat caaaaaggaa ccttcaccgt cggcaacagg ctggtatcgg
     3661 aagaagagcg ttccgtgcaa gccacgcagg agcgctccaa cgccatcact tgcggccacc
     3721 gtgcttgccc gggatgcggt gaagcgctcg gcgcgcgtta cgccctggac gctgcgatgc
     3781 gcgccacggg cgatgatctt atcgccgtgt gtgcgaccgg ctgcctcgaa gttttttcat
     3841 cgcgctatcc cgaaacatcc tggcaaatgg cttggcttca ttcccttttt gggaatgcgc
     3901 ccgcggttgc aacgggcatt gccgccgcca tgcgggtgaa aaagcgcgcg acgcgagtca
     3961 tcgcacaggg cggcgacgga ggcacgaacg acatcggctt ccaatgcctt tccggcctgt
     4021 tcgagcgaaa cgatgacgtg ctctacgtct gctacgacaa cgaaggctac atgaccacgg
     4081 gcgttcagcg ttcgtcggcg acgccgccag ccgcgggcac cgcgacgacg ccagcggtcg
     4141 gaccgtcgcc cggcaacgtc ttcggtacag gcaagtccat gccgaaaatc gcacttgctc
     4201 acggcattcc ctacgttgcg acggcgtcgg tcgccgatct gcacgatctt gagcgcaagg
     4261 tcacgaaggc gatgtccatt catggtgcgc gctacattca gatccacgtg ccgtgcccat
     4321 tcggctgggg agcggcttcg cacgatacga tccggctcgc gcggcttgcc atcgaatgcg
     4381 gcttttatcc catcttcgag gccgagcatg gcaaggtcac gcatgtccgc aagatccgcc
     4441 gcaaggttcc ggtgcaatac tatctgatgc ttcagcgccg cttcgcgcac cttttccgtg
     4501 acggcaagcc agacccgagc gtcgcccgct tgcaagcgat ctgcgatgcc aatatcgccg
     4561 attatggctt gctcgat
//
LOCUS       DATMGX010000465         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_149543, whole genome shotgun sequence.
ACCESSION   DATMGX010000465 DATMGX010000000
VERSION     DATMGX010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149543"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /gene="rsmH"
                     /locus_tag="VNR65_07545"
     CDS             <1..629
                     /gene="rsmH"
                     /locus_tag="VNR65_07545"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWJ18562.1"
                     /translation="HVAPADADGLLADLGVSSMQLENAERGFSFQAEGPLDMRMNTQS
                     GQTAEQVVNRFDERELADAIYEFGEERRSRRIARAIVRARPIHTTAQLAGVISAAARP
                     MNQAERRIHPATRTFQALRILVNRELDDLRELLSAGAAPQVLRPGGRLVIISFHSLED
                     RIVKDALREGARDGVYRVLTKKPATADQEEVDRNPRSRSAKLRAAERT"
     gene            807..1298
                     /locus_tag="VNR65_07550"
     CDS             807..1298
                     /locus_tag="VNR65_07550"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HWJ18563.1"
                     /translation="MAAGVLYPTMAQVGEWNEQPAEQPRGMFIGTPEIYFAKRIDNSR
                     VAKVADPKRRREMISFGITLSVLFLFVMVYLWQHFSAIEYGYRIEQLAAQRNTISESN
                     RALSLEEASLKDPARIDRLAKQMGMQRPAGGQLQPMDSAIADPSTPVMAQANGIIVIS
                     AQP"
     gene            1575..>2258
                     /locus_tag="VNR65_07555"
     CDS             1575..>2258
                     /locus_tag="VNR65_07555"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HWJ18564.1"
                     /translation="MVKFGFFEDLAERQRTRTVPVEPRRGNIYDRYGHPLAMSIDVDS
                     VYAVPIEVHDKETTAAMLGKVLNLDPQDLLVRLKMARPFVFIARRIDAETSNRIRQLN
                     LRGIGFRKEPKRFYPKRDLAAQVLGYVGMDGEGLGGLERGFDDDLRGIPGKELVSLDA
                     RGKWFSRVEKPPDPGQNLVLTIDQTIQYIAERELEQGMEDTKSIAGTVVVENPRTGEI
                     LALANRPTFN"
BASE COUNT          495 a          641 c          707 g          415 t
ORIGIN      
        1 agcatgtcgc tcccgccgat gccgacggtt tgttggccga cttaggcgtc agttccatgc
       61 aattagagaa cgccgagcgc ggatttagtt ttcaggcgga aggccccttg gacatgcgga
      121 tgaatacgca gtccgggcaa accgccgaac aagtggtaaa ccgcttcgac gagcgcgagc
      181 ttgccgacgc gatttacgaa ttcggtgagg aaaggaggtc gcggagaatc gccagagcca
      241 ttgttcgggc gcggccgatc catacaaccg cacaacttgc gggagtgatt tcggccgcgg
      301 cccggccaat gaaccaggct gagaggcgca ttcatccggc gacacgaact ttccaggcac
      361 tccgaattct agtgaaccgt gaactcgacg acctgcggga gctgctgagt gcaggcgccg
      421 cgccgcaggt actcaggccg ggcggcaggt tggtgatcat cagcttccac tcgctggaag
      481 accgcatcgt gaaagatgcg ctgcgcgagg gagcgcgcga cggtgtatat cgcgtgttga
      541 ccaagaagcc ggcgacggct gaccaggaag aagtcgatcg caacccgcgg tcgcgcagcg
      601 cgaagttgcg cgcagcggag cggacgtaga gcgagatcgg ttaaatggtt tcgaaagttc
      661 gcaactcttc acgcgatgtt tgtggaggct gcggaaaaag ctgtgcagaa tgtgaataag
      721 tgcgggccct gaaattctgc tggtgcgttt gaagatacaa gccggacaaa ggccgatgac
      781 gtcaggccgt tcggcaggag agagtaatgg cagccggagt tctttacccg accatggcgc
      841 aagttggtga atggaatgag cagccggcgg agcaaccgcg tggcatgttc attggcacgc
      901 ccgaaatcta tttcgccaag cgcatcgaca actcgcgcgt ggccaaggtg gccgacccca
      961 agcgccgccg cgagatgatt tcgttcggca tcaccttgag cgtgttgttc ctgttcgtga
     1021 tggtgtacct gtggcaacac ttcagtgcga tcgagtacgg ctatcgcatc gaacaactgg
     1081 cagcgcagcg caacaccatc tccgagtcga accgcgcatt gagcttggaa gaggcctcgt
     1141 tgaaggaccc agcgcgaatc gacaggctgg ccaaacagat gggtatgcag cggccggcgg
     1201 gcgggcaatt gcagcccatg gattcggcaa tcgccgatcc atccaccccg gtgatggcgc
     1261 aggccaacgg aatcatagtg atctcggcgc aaccttaaga tgagggcgct caaggcaccg
     1321 tacgaaatcg gctgaaccag gtacggtgaa gtaaagtggc ggccgcgagc aacgggccgc
     1381 cttctccatg taagctgcca gcggtacgac gcgatcattc agagcctcgg cgcgcacgcc
     1441 gaagctcagc agaacggagt gaagagggaa tgcgggccag cgctcccaag acgccaagcc
     1501 gaaggctcta catctttgcc gtgctgctat tcgtttggat atttgccatc tgctttcgcc
     1561 tggttcgatt gcaggtggtg aagttcggtt tcttcgaaga cctcgctgag cggcaacgca
     1621 cccgcaccgt tcccgtcgaa cctcgccgcg gcaatatcta tgaccgctat ggccatccgt
     1681 tggccatgtc aattgacgtc gattcggtat acgcggtgcc gatcgaagtc cacgacaagg
     1741 aaaccacggc ggcaatgctc ggcaaagtgc tgaatctcga tccccaggac ctcctggtgc
     1801 ggctcaagat ggcgcgtccc tttgtcttca tcgcgcgccg gattgacgcc gaaaccagca
     1861 accgcattcg ccagttgaac ctgcgtggca ttggctttcg caaagaaccc aagcgcttct
     1921 atcccaaacg cgacctggct gcccaagttc tgggttatgt cggcatggac ggcgaaggcc
     1981 tgggcggcct ggagagggga ttcgacgacg atctgcgggg cattcccggc aaagaactgg
     2041 tctcgctcga cgcccgcggc aagtggttca gccgggtgga gaagccgccc gatcccggac
     2101 agaatttggt cttgaccatc gaccagacca tccagtacat cgccgagcgc gaactggagc
     2161 aggggatgga agataccaag tcgatcgccg ggactgtggt cgtagagaat ccgcgaaccg
     2221 gcgagattct cgcgctggct aatcgtccaa ccttcaac
//
LOCUS       DATMGX010000466         3767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_49771, whole genome shotgun sequence.
ACCESSION   DATMGX010000466 DATMGX010000000
VERSION     DATMGX010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49771"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..2564)
                     /locus_tag="VNR65_07560"
     CDS             complement(138..2564)
                     /locus_tag="VNR65_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HWJ18565.1"
                     /translation="MDPLRLRLRPAIALLLFCLVLTSSIPLLAQKASAPAVTPSTTAK
                     EQDRFAPLDALLKDAVEKGRAPGAVLLVGHNGTFVYRKAYGYRSLDPSKEPMTVDTIF
                     DMASLTKVMATTSCVMRLVQLGQVKLNDPVAKYIPEFAQNGKDDVTVRQLLTHYSGLR
                     PDLDLKKYWSGLEEGYNRANAEKLINPPGSVFVYSDINFVVLGELVQKVGGMPLDQYS
                     QTYIFGPLGMTWTRFLPPAEWKPRIAPTERDERSGQMLRGVVHDPTARQMGGVAGDAG
                     VFSTADDVAKFAQALLNGGAPVFSPWIVEKMTTPQQPPNLTDVRGLGWDIDSPFSSNR
                     GELLPVGSFGHTGFTGTSLWIDPTTNTYIILLTNAVHIKEGNVITLRTEVATAVAAAL
                     QLQPSEEQKVRVARITGYNEAAAASRRVVVRNGQVSNGIDELEARDFDALRVPSAGTP
                     KVGLVTNQTGVDSHGRRTIDILAHTRGLQLSAIFSPEHGVQGTSDSTNIGDTKDGATG
                     VAVYSVYGDSDAKRRPPLDVLRKLDVLVFDIQDAGARVYTYETTLGYFLEAAATLAKP
                     VVVLDRPNPITGAYVQGPVSDKGQESFVNYYPVPVRHGMTIGELAKLYNTERNINANL
                     TVVPMKGWMRGDWFDSTSELWVNPSPNLRDLNQVTLYPAVALIEQTNVSVGRGTDTPF
                     EVVGAPWVSAKRLAAYLNGRNIAGVRFVPTMFTPTSGPYVHQQCEGIHIIVTNRETLD
                     APELGLELAAALHDAAPTGFDIGRMKPLLANQAAFTALQAGQDPRRIADDWRDGVEQF
                     MQMRKKYLIY"
     gene            2920..>3767
                     /locus_tag="VNR65_07565"
     CDS             2920..>3767
                     /locus_tag="VNR65_07565"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWJ18566.1"
                     /translation="MILNQLSLQPADLPSALDLQDKQFSRAFAMVREAIEQRAFPAAA
                     LAVTHRGSLVALQGFGRFTYEDDAPVVQPDTIFDLASVTKVVATTAVAMLLHERGQLP
                     LDIPLGHFLPDFVARAPRHQQATREAVTLLMLLAHSSGLPAYEKLFEVAASRDELVRA
                     ALTTRLVAAPGTRAEYSDVGFILLGEVLTRQAGVALDLFARQEIFTPLGMAHTRFNPP
                     AEWKPRIPPTEDDRAFRKRIIQGEVDDENASVMGGVAGHAGVFGPAIDIARFAECMLR
                     GGAPIL"
BASE COUNT          715 a         1182 c         1099 g          771 t
ORIGIN      
        1 gccagcggtg gcgctctcgg cttggccacc gttctgttca cgcgcgggcc cgagattcag
       61 ctcggggtag gagattcgat agaaatggtg ctggagcgca gtctgacgat cgacgaaacc
      121 aaggtccgca gagctgctca atagatcaaa tacttctttc tcatctgcat gaactgctcg
      181 accccatcgc gccagtcgtc ggcaatgcgc cgtgggtctt gtcccgcttg cagcgccgtg
      241 aaggccgcct gattcgccag caacggtttc atacggccga tgtcaaaccc cgttggagcc
      301 gcgtcgtgca aagccgccgc caactccagc cccaactcgg gagcgtccag ggtctcgcgg
      361 ttggtgacga tgatgtggat tccctcgcac tgctggtgaa catacggccc gctggttggc
      421 gtgaacatgg ttggcacaaa ccgtaccccg gcgatgttgc gcccattcag ataagccgcc
      481 agccgcttcg cgctgaccca tggcgctcct accacttcga aaggagtatc ggtgccccgg
      541 cctaccgaaa cattcgtttg ctcgatcaac gccacggctg gatacagcgt cacctgattc
      601 aggtcgcgca agttgggcga aggattcacc cacagctccg acgtcgagtc gaaccaatcg
      661 ccgcgcatcc agcctttcat cgggaccacc gtgagattgg cgttgatgtt ccgctcggtg
      721 ttgtacagct ttgccagctc gccaatcgtc atgccgtgcc gcaccggcac gggatagtaa
      781 ttcacaaagc tctcctggcc tttatcggag accggaccct gaacgtaggc tccggtaatc
      841 gggttaggac ggtcgagcac cacgaccggt ttcgccagcg tggccgccgc ttccagaaaa
      901 tatcccagcg tcgtctcgta ggtgtagact cgcgcgccgg cgtcctggat atcgaacacc
      961 agcacgtcga gcttgcgcag cacatcgagc ggcgggcggc gcttggcatc gctgtcgccg
     1021 tacacgctgt aaacggccac tcctgtcgcc ccatctttcg tatcgccgat gttggtgctg
     1081 tcggaggtcc cctgcacacc gtgttcgggg ctgaaaatgg cgcttagctg caaaccacgc
     1141 gtatgggcca gaatgtcaat tgtgcggcgg ccatgactgt cgaccccggt ctggttggtc
     1201 accaatccca ccttgggcgt cccagcactg ggaactcgca aggcgtcgaa gtcgcgcgcc
     1261 tccagttcgt caatcccgtt agacacctgg ccgttgcgga ccaccacgcg gcggctggcg
     1321 gctgccgcct cgttatagcc ggtgatcctc gccacccgca ccttctgctc ttcgctgggc
     1381 tgcagttgca aagcagcggc gaccgcagtg gccacctcgg tgcgcaaggt aatcacgttg
     1441 ccttccttga tgtgcaccgc gttggtcagc aggatgatgt aggtattggt ggtcgggtcg
     1501 atccatagcg aagtgccggt aaagccggtg tgtccgaagg atcccacggg taacaattca
     1561 ccccgattgg aagagaacgg tgaatcgatg tcccaaccca gtccgcgcac gtcggtcagg
     1621 ttgggcggct gttgcggggt ggtcatcttc tcgacaatcc agggagaaaa cactggcgcg
     1681 ccaccgttca gcagcgcttg ggcgaacttc gcaacatcat cggcggtcga aaacactccg
     1741 gcgtcaccgg caactccgcc catctgccgc gcggtaggat cgtgcaccac gccgcgcagc
     1801 atctgtcccg agcgctcgtc gcgttcagtg ggcgcgatcc gaggcttcca ctccgccggc
     1861 ggcagaaagc gtgtccacgt catgcccagc gggccgaaaa tatacgtctg cgaatattga
     1921 tcgagcggca tgccgccgac cttctgcacc aattcgccca gcactacgaa attaatgtcg
     1981 ctgtatacga acaccgagcc tggcggatta atcagctttt cggcgttagc gcggttataa
     2041 ccctcctcca ggccgctcca atacttcttc aaatccaggt cgggccgcag gccggagtag
     2101 tgcgtcaaca actggcgaac cgttacatcg tctttgccgt tttgcgcgaa ctcgggaatg
     2161 tacttggcga ctggatcgtt gagcttcacc tgtccaagct gcaccagacg catcacgcaa
     2221 ctggtagtgg ccatcacctt ggtgagcgat gccatgtcga aaatggtatc gacggtcatc
     2281 ggctccttgc tgggatcgag cgagcgatag ccgtaagcct tgcggtagac gaaggttccg
     2341 ttgtggccga ccagcagcac cgctcccggc gcacgccctt tctcgacggc gtctttgagc
     2401 agcgcgtcga ggggcgcaaa gcggtcttgc tccttggcgg tggttgacgg cgtgacggca
     2461 ggagcggacg ccttctgcgc gagaagggga atgctgctgg ttaggacaag acaaaacaga
     2521 aggagcgcta tagctggccg aagacgaagc ctcaatggat ccaaaacctt tcttgattca
     2581 gggaaactct gaacacgtcg atgtccgggt atgccggctt ataggccggc gtccaagccg
     2641 ttgtaatatt cccgggcttt atttgactgc tgcataagca atgtccggct tcgcgagttt
     2701 gagttgtcat cacgaacggc cttcaggccg tgagggatct gctgtttcct cagcggtcgt
     2761 tccagcgcta acaaaagcag attcctcgct tcgctcgtaa tgacaactcc agagacgaat
     2821 cgtccatccc aaatgggaca ttacttacac ggcgggcttt agcccggaaa cccgcttatt
     2881 ctcccggcaa gctattataa gttgcgatct catctcgagg tgattctgaa ccaactgtcg
     2941 ctacagcccg cagacctgcc atctgctttg gacctccagg acaagcagtt ctcgcgcgcc
     3001 tttgccatgg tccgcgaagc catcgagcaa cgcgctttcc ctgccgcagc gctcgcggtc
     3061 actcatcgcg gatcgctggt cgcgctgcaa ggatttggcc gcttcaccta tgaggacgat
     3121 gcgccggtag ttcagcccga caccatcttc gatctggcgt ccgtgaccaa ggtcgtggct
     3181 accaccgccg tggccatgct gctgcacgaa cgcggacaat tgccgctgga cattcccctg
     3241 ggacacttcc tccccgattt tgtggcacgg gccccgcggc atcagcaggc cacgcgagag
     3301 gcggtcaccc tcctcatgtt gctcgcccac tccagcggac tgccggctta cgagaagctg
     3361 tttgaggttg ccgcctctcg cgacgaactc gttcgggccg cgcttactac gcgtctggtt
     3421 gccgcgcccg gcacgcgcgc cgagtacagc gacgtgggct tcatcctgct gggagaagtg
     3481 ctgacccgcc aagctggcgt ggcgctcgac ctctttgcgc ggcaggaaat ctttacgccg
     3541 ctcggcatgg cgcacacgcg attcaatccg ccagcagagt ggaagcctcg gattccgccc
     3601 accgaagacg atcgcgcttt tcgcaagcgt atcatccagg gcgaagtaga tgacgagaac
     3661 gcttccgtga tgggaggcgt cgcaggacat gccggcgttt tcggtccggc aatcgacatc
     3721 gcgcgcttcg ctgaatgcat gctgcgcggc ggagcgccca tcctcaa
//
LOCUS       DATMGX010000467         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_299481, whole genome shotgun sequence.
ACCESSION   DATMGX010000467 DATMGX010000000
VERSION     DATMGX010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299481"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            527..1711
                     /locus_tag="VNR65_07570"
     CDS             527..1711
                     /locus_tag="VNR65_07570"
                     /inference="COORDINATES: protein motif:HMM:NF006552.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HWJ18567.1"
                     /translation="MSKRDVVVLSAARSAVGTFSGALSDIEPSELAGIVMKETIRRSG
                     ADPKLINYVTVGNTIPTESRSAYVARVASIQAGLPMESVAMAVNRLCSSGLQAIVTTA
                     QNILLGDCDFGIGGGVEVMSRGAYISPAMRSGARMGDTTLVDMMVATLTDPFGVGHMG
                     ITAENLATKWVISREEQDAFAAESQRRSAAAVKDGRFKSQIVPVVKKTRKGEVVVDTD
                     EHVKPDTTVESLAKLRPAFKKDGTVTAGNASGINDAASFFVLADAAAAKAAGLKPTAR
                     LVSYAVAGVPNEIMGEGPIPASKLALKKSGLSLAQMDVIEANEAFAAQAIAVARGLDL
                     DMEKTNPNGGAIALGHPIGASGAVIATKALYELERIGGRYALVTMCIGGGQGIATIFE
                     RL"
     gene            complement(1788..>2019)
                     /locus_tag="VNR65_07575"
     CDS             complement(1788..>2019)
                     /locus_tag="VNR65_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18568.1"
                     /translation="GATRISGISKFKRRVDAEPKRKLGQSSRPWRNHDLRRTMSTHMR
                     DFLGVEKDVVELISKLYTRGPAENSEVAFWRN"
BASE COUNT          423 a          591 c          600 g          405 t
ORIGIN      
        1 aatcagagcg acggtaacga agtctcaatt tgtaattttg aagtcgaatt gagcaatgtt
       61 tcttacagtc gagccgtggt ctgatcaggt gatgggaaca aaattctagc cgtgctcgag
      121 gcgaacgact tctcacacag cctcgggcct tgcctggttt caacggacag gcagacttgc
      181 tgtcctgggc caagagtcag cctgcgctcg aaaatatggg gtgcgatatc gcgtttcgac
      241 acaccccggt cacatccttc gcaaacgccc cattgccgtt ctcgcctatg cggtggcaaa
      301 gccgatcagt ccttgccggg cgctaaccgc gcacccctcg gggcgagcgt ttcaccttaa
      361 tccactcatg accgcttgcg tcactttcga cgatggcgtg ggttatgacc tgcgacccgt
      421 ccaatttgca gtgcaagaat gcgggaatac tcagtataag atagggccgc agctcagagg
      481 atcatgtccg gcggctgaaa agtaacatgt ctaagggagc agaagcatga gtaagcgtga
      541 tgttgttgtt ttgagtgcag cacgttcggc ggttggcacc ttcagcggag ccctttccga
      601 tattgaacca tccgaactgg cgggcatcgt gatgaaagaa acgatccgcc gctccggtgc
      661 tgatccgaag ctcattaatt atgtgaccgt gggcaacact atcccaaccg agagccgctc
      721 ggcctatgtc gcgcgtgttg cctcgatcca ggccggcctg cccatggagt cggtcgcgat
      781 ggcggtgaac cgcctctgct cgtcggggct ccaagccatc gtcacgaccg cccagaacat
      841 tctgctcggc gattgcgatt ttggcatcgg cggcggcgtg gaggtcatga gccggggcgc
      901 ctatatttcg ccggccatgc gctctggcgc tcgcatgggt gacacaacac tggtcgacat
      961 gatggtcgcc acgctcaccg acccatttgg cgttgggcac atgggcatca cggctgagaa
     1021 tctggccaca aaatgggtca tctcgcgcga ggagcaggac gcgttcgccg ccgaatcgca
     1081 acgccgctcg gcagccgcgg tcaaggacgg ccgcttcaaa tcgcagatcg tgcccgtggt
     1141 caagaaaacg aggaaaggcg aggtcgtcgt cgacaccgac gagcatgtga agcccgacac
     1201 gacggttgag tccctggcta agctcaggcc agcctttaag aaggacggca cggtgacggc
     1261 cggtaacgcc tcaggcatca atgacgctgc ctccttcttc gtgctggccg atgccgcagc
     1321 cgcgaaagcc gcaggcctaa agccaacagc ccgtctcgtt tcttacgccg tggccggagt
     1381 accaaacgaa atcatgggcg aaggcccgat tccggcctcg aagctcgcgc tcaagaagag
     1441 cggactctca ctcgcccaga tggacgtcat cgaggccaac gaggcgttcg cggcacaggc
     1501 catcgccgtg gcgcgcggcc ttgacctcga tatggagaag accaacccga atggcggggc
     1561 catcgcgctt ggccatccca tcggtgcgtc cggtgcggtg atcgcgacca aggcgctcta
     1621 tgaactggag cggattggcg ggcgttacgc gctggtcacg atgtgtatcg gcggcggaca
     1681 ggggatcgcg acgatcttcg aacggctcta aacgggcggc gctggcgctg ccatgctgtg
     1741 aacggcgaaa taaacgtccg gctccccgtc acttggggga gccattttca attccgccaa
     1801 aaggcgacct ctgaattctc ggcaggccct ctggtataga gttttgaaat aagctcaacc
     1861 acatcctttt caacaccgag aaagtcgcgc atatgcgttg acatggtacg tcgtagatcg
     1921 tgattgcgcc atggtcggga agattgccca agcttacgct tcggctccgc atccactctg
     1981 cgcttgaact tagagattcc agaaattcgt gttgcgccc
//
LOCUS       DATMGX010000468         2167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_429852, whole genome shotgun sequence.
ACCESSION   DATMGX010000468 DATMGX010000000
VERSION     DATMGX010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429852"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1769)
                     /locus_tag="VNR65_07580"
     CDS             complement(162..1769)
                     /locus_tag="VNR65_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWJ18569.1"
                     /translation="MPVIASRILPQSETFKANAAQLLTQVEKFRSFERAVIAHSSQQA
                     EKFAKRSQILPRERVARLLDHGTPFLELSTLAGFGMHDDDGAARVMGGGTIMGIGVVS
                     GKRAVILASDSAIKGGTISPMGLKKSLRAQEIAKENKLPLIYLVESGGANLLYQSEMF
                     VEGGRSFANQARLSAAGIPQIAVVHGSSTAGGAYLPGLSDYVVLVKEKSKIFLAGPPL
                     VKAAIGEEASDEELGGADLHASTTGLGEYLARDDAHALAIARDLLAKLKWDRVPRREK
                     GAQPKYDAEELLGIVPISEREPYDVREIIARLVDGSDFLDFKRDYGAETVCGYASIDG
                     FEVGILGNNGAIQPDGSTKAAQFIQLCDQAGMPLVFLQNTTGYMVGREAERTGAVKHG
                     SKMIQAVANARVPKLTVIVGGSYGAGNYGMCGRGFDPRFIFSWPNARVAVMGGPQAAK
                     VMEIITRAKFEKLGQVNEPVLKQMSEAIERQLDRESTALYGSARLWDDGIIDPRDTRK
                     ILSLCLAIAAEGDARVLHPNTFGVARL"
     gene            complement(1921..>2167)
                     /locus_tag="VNR65_07585"
     CDS             complement(1921..>2167)
                     /locus_tag="VNR65_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ18570.1"
                     /translation="IPKLKDHVPLRRLGTESEVSAAICFLLSEGAAFISGVTLPIDGA
                     APLRNALMDMGAYKPAPEFQGFHRAKTPEILKKDEDE"
BASE COUNT          400 a          688 c          650 g          429 t
ORIGIN      
        1 gagtgctgcc agcgttgggc tcttcgtgaa agccgttgtt agaacctacc tgcttttgat
       61 aaaatcaaaa gcaggtaggt tctaagtttt gttttgtcgt gcgtcttatc ggaaaaccgc
      121 ttcgcacttt tccggacgca ctaagcgaag ggtgtcgaat tttataatcg cgcaaccccg
      181 aatgtgttcg gatgtaagac acgagcgtcg ccttccgcgg caattgcaag gcaaagcgac
      241 agaattttgc gggtgtcgcg cgggtcgatg atgccgtcat cccacagccg cgcgctgccg
      301 taaagcgccg tcgactcccg gtcgagctgc cgctcgatcg cctccgacat ctgcttcagc
      361 accggctcat tcacctgccc caacttctcg aacttcgccc gcgtgatgat ctccatcacc
      421 ttcgccgcct gcggtccacc catgacggcg acgcgcgcgt ttggccagga aaagatgaag
      481 cgcggatcga agccgcgccc gcacatgccg taattgcccg cgccatagga gccgccaacg
      541 atcaccgtta gcttcgggac gcgggcatta gccaccgcct ggatcatttt cgagccatgc
      601 ttcacggcgc ccgtgcgttc ggcttcgcgg cctaccatgt agcctgtcgt gttctgcagg
      661 aagacgagcg gcatgcccgc ctgatcgcaa agctggatga actgggcggc cttggtcgag
      721 ccgtccggct ggatcgcgcc gttattgccg aggatgccga cttcaaatcc gtcgatggac
      781 gcatagccgc acacagtctc cgcaccgtaa tcgcgcttga aatccaagaa gtcggagccg
      841 tccacgagcc gcgcgatgat ctcgcgcacg tcgtagggct cgcgctcgga aatcggcacg
      901 atgccaagaa gttcttcggc gtcatatttc ggctgcgcgc ctttctcgcg gcgggggacg
      961 cgatcccatt taagtttcgc caagagatcg cgcgcgatcg cgagagcatg cgcatcatcg
     1021 cgcgcgagat attcgccaag cccggtcgtc gatgcgtgaa ggtccgcgcc gccgagttcc
     1081 tcgtcggagg cctcttcgcc gatggcggcc ttcaccagcg gtggaccggc caggaagatt
     1141 ttggacttct cctttaccag gacgacatag tccgagaggc cagggagata agcgccgccc
     1201 gcggtcgatg acccatgcac gacagcgatc tgggggatac ccgctgccga aagccgagcc
     1261 tggttcgcga aggaacgccc gccttccacg aacatctcgc tttggtaaag caggttcgcg
     1321 ccgcctgact cgacgagata gatcagcggc agcttgtttt ccttcgcgat ctcctgcgcg
     1381 cgcagcgact ttttcagtcc catcggcgag atggtgccgc ctttgatggc gctgtcgctc
     1441 gccaggatca ccgcgcgctt gccggaaacg acgccgatgc ccatgatggt gccgccgccc
     1501 atgacgcgcg ccgcgccatc gtcgtcgtgc atgccgaagc ccgcaagcgt ggacagttcg
     1561 agaaacggcg ttccatgatc gagaagccgg gcgacgcgct cccggggcag aatttggctt
     1621 cgtttcgcga acttctcggc ctgctgcgac gaatgcgcga tcacggcgcg ctcaaagctg
     1681 cggaattttt ccacctgcgt cagaagctgc gccgcgttgg ccttgaaggt ctcggattgc
     1741 ggtaagatcc gcgaggcgat cacgggcata ggcggcctcc tcggcaccaa ctcgatgtca
     1801 tagccgccct tgaggcggct atccagggca gccaggcaag cgttcgagcc ttgggctgga
     1861 tggccgggtc aagcccggcc atgacggaag acgaggcgcg ggaatgttgg tcgagcccgc
     1921 tcattcgtct tcgtccttct tcaggatctc gggcgtcttc gcgcgatgga atccctgaaa
     1981 ctcaggcgcg ggcttgtatg cgcccatatc catgagcgca tttcgcaacg gcgcagcccc
     2041 gtcgataggc agggtcacgc ccgagataaa ggccgctccc tcgctcaaga ggaaacagat
     2101 cgcggcgctc acttccgact ccgttccaag acgcctcagc ggcacgtggt ccttcagctt
     2161 cgggatc
//
LOCUS       DATMGX010000469         2656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_351006, whole genome shotgun sequence.
ACCESSION   DATMGX010000469 DATMGX010000000
VERSION     DATMGX010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351006"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VNR65_07590"
     CDS             <1..615
                     /locus_tag="VNR65_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HWJ18571.1"
                     /translation="APIAPAAAPALPPKIAIADTYANPAPLGLLCFALTTILLSAHNA
                     GLFALDAMIVAMAIFYGGLAQIVAGIMEWRKNNTFATTAFISYGSFWLSLAGIIILPK
                     LGLAEKASPTAMAAYLGIWGLMSFIMFIGTLRLNRALQVTFFLLTIAFALLSIGDLTG
                     NKVLTTIAGWEGMLLGFSAMYTGLAQVLNEVYGRVVWPLGPVQK"
     gene            1901..>2656
                     /locus_tag="VNR65_07595"
     CDS             1901..>2656
                     /locus_tag="VNR65_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ18572.1"
                     /translation="MTAATLQALLYSPPRPVYLADRPNLRQFLRAARTNALLIWPEAA
                     YERDFLVNRLLGRTQILINAPEAIHRVLVENPGNYRRTPASIRILRPIAGDGVLLSEG
                     ESWRHQRRTIAPSMAPRMMPMLARHIADVAEEVVTSLRQRSKAPLDLLQEMQFAALEI
                     AGRSMFSLEMGVFGPEMRRMLTDYGIRLSQPHLLDMLLPPSIPAPRDFARRRFQRRWM
                     ALIDRVIDERLASGEDNEARDLFDLLRAARDPET"
BASE COUNT          544 a          771 c          753 g          588 t
ORIGIN      
        1 gccccgattg cacctgcggc agcgcccgct ctccccccga agatcgccat cgcggacact
       61 tatgccaatc ccgctccgct ggggctgctc tgtttcgcgc tgacgaccat tctgctcagc
      121 gctcacaatg ccggcctgtt cgctctcgac gcgatgatcg tcgccatggc catcttctac
      181 ggcggcttgg cgcagatcgt ggcaggcatc atggagtggc ggaagaacaa taccttcgcc
      241 acaacggcct tcatctccta cggttctttc tggctgagcc ttgccggcat cattatcttg
      301 ccaaagcttg gcttggccga gaaagcaagc cccacggcga tggccgccta tctcggcatc
      361 tggggcctga tgtcattcat catgttcatc ggaaccctca ggttgaaccg cgccctgcaa
      421 gtcacgttct tcctgctgac gatcgcgttc gcgcttcttt ccatcggcga cctcaccggc
      481 aataaggtgc tgacgacaat tgcgggctgg gaaggcatgt tgcttggatt ttcggcgatg
      541 tatactggcc ttgcgcaagt tctgaacgaa gtttacgggc gggttgtctg gccgctcgga
      601 cccgttcaga agtagtcagg cagccgccgc gcaggttaag cgcggcggct ctccgtttga
      661 aagcgagacc gctctgatgc tccaggccgg atttcagagc gctactctga cacgaacacc
      721 ctggcctgaa gcccgggtag ccggatgtca atcacaagga tagatttgat gtcccaatac
      781 agtgcaattc tggacccggt taatgtatag cccgcttgca tcctggttga agtaatccct
      841 agaccgcacc gcaggtccat cttggagtcg atggccgaca gccaggaaag tttctggtat
      901 ctcttgtgcc tgaaccaccg cgacagttcc gattgagata gaagaaaagg caacaacagt
      961 cgctattatg gaatgcatat gagcctccac aggccagttt agtcaaccga ttgtcgtctg
     1021 tcgctttcgt caaaaccgcg ccttggaggc gtggttgagt gcagcgctta accaccgttt
     1081 gagctacacg catacgaagt gggaaggccg gtagcacgac tgaagcagga actatcccgg
     1141 tcgcgcaccg catcgtaatc caagcgattg taatcgaccg cgctctgcgt tgggctcggc
     1201 cgttgggcat gtgttctagc atccgatact gtgacatagg atagaaccag taaaccggcg
     1261 ccaattcccg ttataaaggg tctcatgttt agcctcctcc tcacgggaga atctaatgaa
     1321 tcccatctac tttttccggg gtactattcg cacgttccgt cttaaggagc ctagttccag
     1381 attatgatcg atggaataga gttgaacgga tctcccgttt ttctcgtcgt tacgctgctt
     1441 ttgtcaagct tgaacgtatg cgcataacaa agcttctatt atgcctgcat atcacgcaat
     1501 tactaaacag ctatcatctg aatatatgag gcccgctcaa ttgattgtgt gtcagttttg
     1561 tgacaaatta taaaatttat accataatct tatcttgcca taactagagc ctgagcggcg
     1621 taagtgacct ataaatctag aaaaggccac atggcaggca ggaaggtgga gggaattgaa
     1681 ggatgggcgt gatgggagcg attgtaacga agtcctgaag actaggccgg ccatcgagcg
     1741 gttgcgcaga agctgctcgg acagcaagta tcctatcgcg agcatgcaat ggagcccgca
     1801 ggcaatcagc ctcggggggc gtatcaggca gtgccgacat gagcgagaac atcggcgcgt
     1861 tcgccagcag ttgcgctggc gacatttggg agccttgcag atgaccgccg caacccttca
     1921 agcgttgctc tatagccctc cgcgtccggt gtacctggct gatcggccga acctgcgcca
     1981 gttccttcgt gcggccagga cgaacgcgct tttgatctgg cccgaagccg cttatgagcg
     2041 cgacttcctc gtgaaccgcc tgctcggccg gacgcagata ctcatcaacg cgccggaggc
     2101 gatccaccgg gtgctggtgg agaaccctgg caattaccgc cggacaccgg cctccattcg
     2161 cattctccgg ccgattgccg gggacggggt gcttttgagc gagggcgaga gttggcggca
     2221 tcagcggcgc accatcgcgc cttcgatggc gccccgaatg atgccgatgc tggcccgtca
     2281 catcgcggac gtcgcggagg aagtggttac ctcgctcagg cagagaagca aggctccgct
     2341 ggatctgttg caggagatgc aattcgccgc gctcgagatc gccggccgtt caatgttttc
     2401 gctcgagatg ggagtcttcg ggcctgagat gcgccgcatg ctgacagatt acggcatccg
     2461 gctgtcacaa ccgcatctgc tcgacatgct gctgcctccg tcgatcccgg ccccgcgcga
     2521 cttcgcccgg cggcgctttc agcgacgctg gatggcgctg attgaccgcg tcatagacga
     2581 gcgccttgca tcgggtgagg acaacgaggc gcgcgacctg ttcgacctgc tccgtgccgc
     2641 gcgcgacccc gagacc
//
LOCUS       DATMGX010000470         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_260850, whole genome shotgun sequence.
ACCESSION   DATMGX010000470 DATMGX010000000
VERSION     DATMGX010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260850"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VNR65_07600"
     CDS             complement(<1..304)
                     /locus_tag="VNR65_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18573.1"
                     /translation="MASFTNALFGGALIGAATAGLLLINGRVAGISGILAAAIKGRPG
                     LWRWAFLAGLIAAGLLGRAVSLPVGSSTGEIPASLPTLLLAGTLVGLGTAIGSGCTS"
     gene            complement(379..1164)
                     /locus_tag="VNR65_07605"
     CDS             complement(379..1164)
                     /locus_tag="VNR65_07605"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ18574.1"
                     /translation="MVAAPAFAHKAAATRALHIRDVSHSFWLEGKPLPVLQDITFSVD
                     AGQFVALLGPSGCGKSTLLRLVAGLDKPLQGEIRTGDAPVEGPDPSRVVVFQDPTLYP
                     WRRVWDNVALGLEAQGLLKTRRPRVDESLDLVGLKAFAKAYPHQLSGGMAQRVAVARA
                     LVNDPEFLILDEPLGKLDSLTRITMQGELVRLWQQKGFTALLVTHDVEEALIMATRVI
                     VLSDRPATIKADIEIDSPYPRHRDDPKLVTLRREILGLLGLEW"
     gene            complement(1158..>2009)
                     /locus_tag="VNR65_07610"
     CDS             complement(1158..>2009)
                     /locus_tag="VNR65_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ18575.1"
                     /translation="RGSEFAAATAFISALLFGAILLGLLHDGIAARARRSLPWSLALG
                     LFILVWQVLASKSGTLPQPFFPPPHALLEVFTDDYAKLFESVVASVKLEMFGFLLGAL
                     AGFLTGVALGWSRTFGYWVHPVMRMIGPLPLIAWLPIAFFAFPSSWSASIFLIALTTG
                     FPVAVLTWSGVASVNTAYYDVARTLGANQRFLIFKVAIPAALPQVFVGLFMGLGASFA
                     VLVTAEMMGVKAGLGFYLQWAQGWAAYANMYAALLVMALMCTGLITLLFKVRDTVLSY
                     QKGDVKW"
BASE COUNT          469 a          600 c          607 g          333 t
ORIGIN      
        1 cgcttgtgca gccgcttccg atcgctgtcc cgaggccgac gagagtgcca gccaaaagaa
       61 gtgtcgggag gcttgcgggg atctcacctg tcgaactgcc caccggcaag ctgaccgctc
      121 ggccaagcaa tccagccgcg ataagcccgg caaggaaggc ccagcgccac aggccaggac
      181 ggcccttgat ggccgccgcg agaatgccgc ttatcccggc cacgcgacca ttgatgagca
      241 aaagccctgc tgtggcggcg ccgatcaagg cgccgccaaa caacgcgttc gtaaaagatg
      301 ccatgccctt attcctcaag gcttggggtt gaacgaattt ccctggagaa cgcgttcaac
      361 cggatatccg ccctttcatt accactcaag gccgaggaga ccgaggattt cccggcgcaa
      421 ggtgacgagt ttaggatcat cgcgatggcg aggatacggg ctgtcgattt cgatatcggc
      481 ttttattgtc gctggcctgt cgctcagcac gatcacgcgc gtcgccatga tgagcgcctc
      541 ttcgacatca tgcgtgacca ggagcgcggt aaatcccttt tgctgccaaa gccgaacgag
      601 ttccccctgc atcgtgatgc gcgtcaacga gtcgagcttt ccgagtggct catcgagaat
      661 caggaactcc ggatcgttga caagagcgcg cgcgaccgca acgcgctggg ccatgccgcc
      721 ggagagctga tgcggatagg ctttcgcgaa cgctttcagg ccgacgagat cgaggctttc
      781 atcgacgcgc ggtcttcttg ttttgagaag gccttgggct tcgagaccaa gcgcgacgtt
      841 gtcccaaacg cgcctccagg gataaagcgt cggatcctgg aacaccacca cacgagacgg
      901 gtccggccct tcgacgggtg cgtcgccggt tcgaatttca ccctgcaaag gcttgtcgag
      961 ccctgccacg agccgcagga gcgtcgactt cccgcatccg ctcgggccca gcaaagcgac
     1021 gaactggccc gcatcgacgc tgaacgtgat gtcctggagg acgggcagtg gcttgccttc
     1081 cagccaaaaa gaatgggaga cgtcgcggat atgcagcgcg cgagttgcgg ccgccttatg
     1141 cgcaaaagcg ggagcggcta ccatttcacg tcacccttct gataggaaag gaccgtatcc
     1201 ctcactttga aaagaagtgt gatcagcccg gtgcacatca gcgccatgac gagcaatgcc
     1261 gcatacatgt tcgcgtatgc ggcccagccc tgtgcccatt gcagatagaa ccccagcccg
     1321 gctttgacac ccatcatttc cgccgtcacg agcaccgcga acgacgcgcc aagtcccatg
     1381 aacagtccga caaagacctg aggcagcgcg gcgggaatcg cgaccttgaa gatgagaaag
     1441 cgctggtttg cgccaagggt acgagccaca tcatagtagg cggtgttcac gctcgcgacg
     1501 ccggaccatg tcaggacggc gaccgggaaa ccggtggtca atgcgatcag gaaaatgctg
     1561 gcgctccaac tggagggaaa agcaaagaat gcgatgggaa gccaggcgat caaaggcaaa
     1621 ggaccgatca tgcgcatgac ggggtggacc cagtagccga aggtgcgcga ccatcccagc
     1681 gctacgccag tgaggaagcc ggccaatgcg ccgagcaaaa agccgaacat ctcaagcttg
     1741 acggaggcga caacgctctc gaacagcttt gcatagtcgt cggtgaagac ttccaacaac
     1801 gcatggggcg gaggaaaaaa aggttgcggc aaagtcccgc ttttcgaggc gaggacttgc
     1861 cagacaagaa tgaatagccc gagcgccagc gaccacggca acgaacggcg ggcgcgcgcc
     1921 gcgatgccat cgtgaagtaa tccgagaaga atggcgccaa acaagagagc gcttatgaaa
     1981 gcggttgccg cagcaaattc tgatccgcg
//
LOCUS       DATMGX010000471         2338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_98902, whole genome shotgun sequence.
ACCESSION   DATMGX010000471 DATMGX010000000
VERSION     DATMGX010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98902"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VNR65_07615"
     CDS             <1..606
                     /locus_tag="VNR65_07615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HWJ18576.1"
                     /translation="MRVLSPDGCFPFSKRRNGTVLGEGAGILVLEEMEQAKARGAAIL
                     AEVLGFGMTSDAIDMVNPDIDGPARAMQIALAEANLAPGDIDYLNAHGTATSANDINE
                     TRAIKKVFGAHANRLSVSSTKSMHGHCLGAAGGVEAVVCVQAMREGYIPATIGFDEPG
                     SECDLDYTPNEGRYRDISYTMSNSFAFGGLNAVLVFGPPRN"
     gene            776..1723
                     /locus_tag="VNR65_07620"
     CDS             776..1723
                     /locus_tag="VNR65_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWJ18577.1"
                     /translation="MFEEHSPLAWVAFNILILAILAADLGVFHRKAREIPVREALWTT
                     AGYITLALSFNAWIYFQFGSVKALEFLTGYFIEYALSMDNILVFVVILSYFQVPAQFQ
                     MRVLLWGILGALVLRGIFILAGFALIEAFEWTVVVLGVFLIYTGFKTMFSGDESIDLK
                     DSRVIRITRRLIPITHEYDGEKYFTKLPSGKAVATPLFLVLIVLNVTDIIFALDSIPA
                     IFAITRDPFIVYTSNVFAILGLRALYFALAGMVDKFRYLKYGLSLVLIFIGFKMIYNY
                     PSWLSDIPTVWALGVTAVLIFGSIFASLVHNKIEGASPQ"
     gene            complement(1746..>2338)
                     /locus_tag="VNR65_07625"
     CDS             complement(1746..>2338)
                     /locus_tag="VNR65_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWJ18578.1"
                     /translation="NTVSDKNPYVVHPLRRSDCALVSDGAAALVLVAEDAAGDAPRAI
                     RFRATAHVNDVLPLSRRDPAVFDGARRAWKLALEKAGVTIGDLSLAECHDCFTIAELI
                     QYEAMGLTALGQGHIAVREGWTEKTGKLPVNPSGGLKSKGHPIGATGVSMHIMASMQL
                     MGEAGEMQIPDAVLAGVYNMGGAAVANFCSILERAR"
BASE COUNT          454 a          703 c          654 g          527 t
ORIGIN      
        1 atgcgcgtgc tctcgccgga tggctgtttt ccgttttcga agcggcgcaa cggcactgtg
       61 ctcggcgaag gcgccggcat cctcgttctg gaagagatgg aacaggccaa agcccgcggt
      121 gcggccattc tggccgaggt attggggttc ggcatgacgt cggacgcgat cgacatggtg
      181 aaccccgaca ttgatggccc ggctcgcgct atgcagatcg ctcttgccga agccaacctc
      241 gcgccgggtg acatcgatta tttgaatgcg cacggcaccg ccacgtccgc gaacgatatc
      301 aacgaaacac gggctatcaa gaaggtgttc ggagcgcacg caaacaggct ttccgtctcc
      361 tccacgaaat cgatgcacgg gcactgcctg ggtgcggctg gcggggtcga ggcggtggtc
      421 tgcgttcaag ccatgcgcga aggctacatc ccggcgacaa taggattcga cgagcccgga
      481 agcgagtgcg acctcgatta cacgccgaat gaagggcgct accgtgacat cagctacacg
      541 atgtcgaatt ccttcgcatt cggcggactg aacgctgtgc ttgtttttgg cccgcctcgg
      601 aattgacatt aaatagcccg gctctgatct aaatgctgag gtgggtaaaa ccctctggca
      661 ggacaagatg cctttaagct cgctctggcc ccaacggcaa caacaatgaa gactgccgcg
      721 ttgtccctgg tagagcgggt gcgagcaggt atgtaaggtt ttttcgatga tctcaatgtt
      781 tgaagagcac tccccccttg catgggttgc tttcaacatt ctcatactcg ccattctggc
      841 agccgacctc ggagtcttcc accgcaaagc acgagagatc ccggtccgcg aggcgctttg
      901 gacaacggcc ggctatatca cgcttgccct ttcttttaac gcctggatct acttccaatt
      961 cgggtctgta aaggccctcg agttcctgac cggctacttc atcgaatatg cgctgagcat
     1021 ggacaacatc ctcgtgttcg ttgtcatcct cagctacttc caggttccag cccagttcca
     1081 gatgcgcgtg cttctttggg gcattcttgg cgcgctggtc ttgcgcggca tcttcattct
     1141 cgccgggttt gccttgatcg aagccttcga atggacggtg gtcgtcctcg gcgtcttcct
     1201 gatctatacc ggtttcaaaa ccatgttttc tggcgatgag tccattgatc tcaaagatag
     1261 ccgtgtgatc aggatcacgc gtcgactcat cccgatcacg catgaatatg acggcgagaa
     1321 gtacttcacg aagcttccat caggaaaggc tgtggcgacg ccgcttttcc tcgtgctcat
     1381 cgtcctgaac gtaacggaca ttattttcgc gctcgattcg attcccgcga tcttcgcaat
     1441 cacccgcgat cccttcatcg tctacacatc gaacgttttc gcgatccttg gtctcagggc
     1501 gctttatttt gccttggcgg gcatggtgga taagttccgc tatttgaaat acggcctgtc
     1561 gctcgtgctg attttcatcg gcttcaagat gatctacaac tacccgagct ggttgtcaga
     1621 catcccgacc gtgtgggcgc ttggcgtaac cgcggtgctg attttcggct ccatcttcgc
     1681 gtctctcgtc cacaacaaga tcgagggagc ctccccgcaa taaatgcggg cgccaggcgg
     1741 ctctgtcagc gcgcgcgctc caaaatcgag caaaagttcg ccacggctgc gccgcccata
     1801 ttgtagacgc ctgccagcac ggcatccggg atctgcatct cgcccgcttc gcccataagc
     1861 tgcatcgacg ccatgatgtg catcgagacc cccgtggccc cgatgggatg gccttttgat
     1921 ttgaggccgc cggaggggtt taccggcagc ttcccggtct tttccgtcca gccttcgcgc
     1981 accgcgatat gaccttgtcc cagagccgta aggcccatgg cctcgtactg gatcagctca
     2041 gcaatggtga agcagtcgtg gcactcggcg agacttaggt cgccaatggt aacgccggct
     2101 ttctcaagcg ccagcttcca ggcacggcgc gcgccatcga aaaccgccgg atcgcgccgg
     2161 gacaggggca aaacgtcgtt cacgtgcgcc gtggcacgaa atcggatcgc gcgcggtgcg
     2221 tcgcccgctg catcttccgc gacaaggaca agcgccgccg cgccatcaga aacgagtgcg
     2281 caatcgctgc gccgcaaagg gtgaacaacg taagggttct tgtcggacac cgtattgc
//
LOCUS       DATMGX010000472         2328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_354622, whole genome shotgun sequence.
ACCESSION   DATMGX010000472 DATMGX010000000
VERSION     DATMGX010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354622"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VNR65_07630"
     CDS             complement(<1..196)
                     /locus_tag="VNR65_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18579.1"
                     /translation="MASQPYLRIADDGTYELAVPAGKGCVHILPYCFHTEEDAMKWLD
                     TRKGREQVNKISGRTHPRFAI"
     gene            complement(520..786)
                     /locus_tag="VNR65_07635"
     CDS             complement(520..786)
                     /locus_tag="VNR65_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HWJ18580.1"
                     /translation="MARQAEDHDAVRVCLDPRLALPKKRSLTIAGHRTSITLEDAFWA
                     ELKGAATEMGLTVTALVAAIDACRAPAGLSAAIRVFLLARRSAA"
     gene            complement(790..2208)
                     /gene="fumC"
                     /locus_tag="VNR65_07640"
     CDS             complement(790..2208)
                     /gene="fumC"
                     /locus_tag="VNR65_07640"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWJ18581.1"
                     /translation="MTSTSSAPSQATRTETDTFGPIDVPANHYWGAQTQRSLGNFKIG
                     GERMPLPLIHALGIVKKCAAKTNAALGQLDTSLADVIVTAAQEVIDGKLDDEFPLVVW
                     QTGSGTQSNMNANEVISNRAIELLGGTMGSKKPVHPNDHVNRSQSSNDTFPTAMHIAA
                     AVQIERSLLPALEHLASAIEQKSVEFERIIKIGRTHMQDATPVTLGQEFSGYATAVRY
                     GMERVKGAMPRMYMLAQGGTAVGTGLNSPAGFAERFADEVARETGLPFITAPNKFEAL
                     SMNDAEVELSGALNALSASLFKIANDIRLMGSGPRSGLAEISLPENEPGSSIMPGKVN
                     PTQCEALTMVCTQVMGNHVTITIAGSQGHFELNVFKPVIICNLMQSIRLIGDAATSFT
                     DNCVSGIVVNEARVKELLERSLMLVTALAPKIGYDKAAHIAKTAHKKGTTLREEALGL
                     GYISEEDFNALMKPELMIKPSE"
BASE COUNT          461 a          661 c          699 g          507 t
ORIGIN      
        1 cgatcgcaaa tcgggggtga gtgcggccgc ttatcttgtt gacttgttcc cggcccttcc
       61 gggtgtccag ccatttcatc gcgtcttcct cggtgtgaaa gcaataagga agaatatgaa
      121 cacagccttt tcctgccgga acggcaagct cgtaagtacc gtcgtccgct atgcgtagat
      181 agggttgaga agccatatgt attacctcgg tgaccagggc ggagtgaaac tgctcacaat
      241 atcaaaacaa ggcagagtac acgaagttcc gccatatttt tgactgaacg gagacattcc
      301 aatcgcccca ccgattgtga aaggcacttt aatatatgtt tttgttcttc ttacgaacaa
      361 tttgagacga aattaatttg aataggtatc gtcgatgaaa ggcctactcc tgcagccctc
      421 agcgcaatag caggtgccgt ggacacatgc tgacccacac ctttgaagat tggcttgcaa
      481 taaccgtaac aattagcgcg gcggcttaac gtttgggaat tatgccgctg atcgccgggc
      541 gagcagaaac accctgatcg ctgcggatag gcccgccggc gcccggcaag cgtcaatcgc
      601 cgcaacaagg gctgtcacag tcagccccat ttccgtagcg gcgcctttga gttctgccca
      661 gaatgcgtcc tcgagcgtga tcgaggtgcg atggcccgcg atagtcagcg agcgcttctt
      721 cggcagcgca agacgaggat caaggcatac gcgaacagcg tcatggtctt cggcttgccg
      781 cgccacagcc tattcgctgg gcttgatcat aagctcaggc ttcatcagcg cgttgaagtc
      841 ttcttccgag atgtagccga gtccaagcgc ttcttctcga agcgtcgttc ccttcttgtg
      901 cgctgttttc gcgatatgcg ccgccttgtc gtaaccgatc ttcggggcga gcgccgtgac
      961 gagcatcagc gagcgctcaa gcagctcctt cacgcgcgcc tcattgacga cgatgcccga
     1021 gacgcaattg tcggtgaagc tcgtggcagc gtccccgatc agcctgatcg attgcatgag
     1081 attgcagatg atgacgggct tgaagacatt gagctcgaaa tggccctggc tgcccgcgat
     1141 cgtgatcgtc acgtgattgc ccatcacctg cgtgcaaacc atggtcaggg cctcgcactg
     1201 cgtcgggttc accttgcccg gcatgatcga agagcccggc tcgttttcag gaagggagat
     1261 ttccgccaac cccgagcgcg gccccgatcc catgaggcgg atatcgtttg cgatcttgaa
     1321 taggctcgcg gacagcgcat tcagcgcgcc ggacaattcc acctcggcgt cgttcatgga
     1381 gagcgcttcg aacttgttcg gggccgtgat gaagggcagc cctgtttcgc gcgccacctc
     1441 atcggcgaac cgctccgcaa atccggcagg ggaattgagc ccggttccga ccgcggttcc
     1501 gccctgcgca agcatgtaca tgcggggcat cgcgcctttc acgcgctcca tgccatagcg
     1561 cacggccgtc gcgtaaccgg agaattcctg accgagcgtg acgggggttg catcctgcat
     1621 gtgggtgcgg ccgatcttga tgatacgctc gaattcgacc gacttttgct cgatggcgct
     1681 cgcaaggtgc tccagcgcgg ggaggaggga gcgctcgatc tgtacggcgg ccgcaatgtg
     1741 catcgccgtc gggaacgtat cgttcgaaga ttgcgagcgg ttcacatggt cgttggggtg
     1801 gacgggcttc ttggagccca ttgttccgcc gagcaattca atggcgcgat tcgagatgac
     1861 ctcattcgcg ttcatgttgc tttgcgtgcc tgacccggtt tgccagacga ccagggggaa
     1921 ctcgtcatca agcttgccgt cgatcacctc ttgcgccgct gtgacgatga cgtccgcgag
     1981 agacgtatcg agctgcccaa gagcggcatt cgtcttcgcc gcacactttt tgacgatgcc
     2041 gagcgcatga atgagcggca gcggcattct ctcgccgcca atcttgaagt tgccaaggct
     2101 ccgctgcgtc tgagcgcccc aatagtgatt ggctggaaca tcgatggggc cgaatgtgtc
     2161 ggtctcggtg cgcgtggctt gagacggggc ggatgaggta gaagtcatgg cggctcgctt
     2221 ttggcaggac aatgcagcgt tctaccacgc gagcgacgca agcaccattg gtcgagttgg
     2281 cgctggcaga gacagtctgg agggggcgca tggaaaggtc cggccggg
//
LOCUS       DATMGX010000473         2206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_286896, whole genome shotgun sequence.
ACCESSION   DATMGX010000473 DATMGX010000000
VERSION     DATMGX010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286896"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VNR65_07645"
     CDS             complement(<1..464)
                     /locus_tag="VNR65_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18582.1"
                     /translation="MQHDTSPHSVEVGGRKYKAQTASGVLCYSRMLFFQIHPTFNRFD
                     CKVFLTDALRHTGGVVERVMIDNTHVVVLRGTGREMIPVPEMEAFADRFGFRFVAHER
                     GDANRSARVERPFWFIESNFLAGRTFASWEDLNNQARQWCDKVNSTYKKYIHA"
     gene            594..1847
                     /locus_tag="VNR65_07650"
     CDS             594..1847
                     /locus_tag="VNR65_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWJ18583.1"
                     /translation="MSIESSSTASRLEEFESHLRTARYAAAIQRRYLWIAQRFVDYLG
                     RKCIAVEAARAPECEDFLRSDLRSWRRRHGRDPRNIFEWRRRHKTAVNVFLRLVHGHW
                     PVAATPVTAIEAFHRDLVQGYDAWMRDLRGLASVTRSTRTTQALVFLTELGPRGEQES
                     LSHLGVRDIDAYLQQRCKGWRRQTIEGYTVCLRSFLRYLHGSGRTALDLSSTVIGPRI
                     YDDEQIPSALRPEEVQAVVEATRQDCSPIGRRDYAFLMLLATYGLRAGEIVALRLEDI
                     DWQKEILRVRHSKTGAVSELPLLREPGEAVLSYLEKARPESKRREVFLQHQAPYQAYK
                     SGSILNCVIGARLKMAGITPRSRKGPHAFRHARAVSLLRASVPLKIIGDVLGHRSAES
                     TAVYLKLATEDLRAVGLNVPKEVLP"
     gene            1991..>2206
                     /locus_tag="VNR65_07655"
     CDS             1991..>2206
                     /locus_tag="VNR65_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18584.1"
                     /translation="MSVSNLQEWLCEKRQMWPLHMVCHRARLVERFLEWSQAIGVIDT
                     NPFAELHRHYGPRTTPIVRALVSDDVEA"
BASE COUNT          436 a          634 c          663 g          473 t
ORIGIN      
        1 gcgtggatgt atttcttgta agtcgagttg accttgtcgc accactgccg cgcctggttg
       61 ttcaaatcct cccaactggc gaacgtgcgg cctgccagaa agttgctctc tataaaccag
      121 aagggccgtt ccacgcgcgc cgagcggttg gcatcgccgc gctcatgcgc cacgaaacgg
      181 aacccgaaac gatccgcgaa cgcctccatc tccggcaccg gaatcatctc gcgccccgtg
      241 ccccgcaaca ccaccacgtg cgtgttatcg atcatcacgc gctcgaccac accgcccgta
      301 tgccgcagcg cgtcggtgag aaataccttg cagtcgaatc ggttaaacgt cggatggatc
      361 tggaagaaca acatccgcga gtagcacaag acacccgagg cggtctgcgc cttgtacttc
      421 ctgccgccca cctcgacgga gtgcggagac gtatcgtgct gcatctccgc gcctaatcgt
      481 aagcatgccg taatgagaag gcggcccgcg ccgtgcagat atacagcact ttaatgagac
      541 tgtcgcttcc cattaagagg gcaggtgcga tcctcggctt ggaggaggtg ctcatgtcca
      601 ttgaatcgtc ttcgactgca tcgcgattgg aagaattcga aagtcaccta agaacagccc
      661 gttatgccgc agcgatccag cggcgctact tgtggattgc gcagcggttt gttgattacc
      721 ttgggaggaa gtgtatcgcc gtcgaggcgg cccgtgcgcc ggagtgcgag gatttcttgc
      781 gatcggacct tcgaagttgg cgtaggcggc acggacggga tccgcgcaat attttcgaat
      841 ggcgtcgccg ccacaaaact gcggtgaacg tgttcttgcg gctggtgcat ggacattggc
      901 ccgttgccgc cacccctgta acggccatcg aggcatttca ccgcgatctg gtacagggat
      961 atgacgcgtg gatgcgggat ttgcgtggct tggcatcagt gacacgctcg acgcgtacaa
     1021 cacaggccct cgtgttcctg actgagctgg ggccgcgtgg cgagcaagag agcctttcgc
     1081 acttgggcgt tcgcgacatc gatgcatacc tgcaacagcg ttgcaagggt tggcgacggc
     1141 aaactatcga gggctacacg gtgtgccttc gaagcttctt gcgctatttg catggcagcg
     1201 gacgaaccgc cctcgatctg agcagcaccg tgatcggacc acggatttat gacgatgagc
     1261 aaattccttc ggcactgcgt cccgaggagg ttcaggccgt cgtggaagcg actcgtcaag
     1321 actgctcgcc gattggccga cgggattacg cctttttgat gctgttggcc acttacggcc
     1381 tgcgagccgg cgaaatcgtc gcgttgcgtc tggaggacat tgactggcag aaagagatcc
     1441 ttcgcgtgcg gcactcgaag acgggcgcgg tttccgaact gcccttgctg cgcgagcccg
     1501 gcgaagcagt actgagctat ttggaaaagg ctagaccgga gagcaagcgt cgagaggtat
     1561 ttctgcaaca ccaggcgccc tatcaggcat ataagagcgg gtccatcctt aactgtgtca
     1621 tcggcgctcg tctgaagatg gccgggatca ctcctcgcag caggaaggga ccccacgcct
     1681 ttcgtcatgc acgagcggtc agtctgctgc gcgcctcggt gcccctaaag atcattggtg
     1741 atgtccttgg gcatagatcc gcggaatcga ctgcggtgta tctgaaactc gcgacggagg
     1801 atctacgggc cgtcggctta aacgttccaa aggaggtgtt gccatgactc gctcatcctc
     1861 ggcggccgcg gactctgttg gccgctttct ccgtactcag cgttttcgcc atagagaaac
     1921 ccgcagaatt tatggccata tcctgcgtgc gtttcagtca tctgtcggcg aatgctccat
     1981 tggtgcgtcg gtgtccgtat cgaatctaca ggaatggctc tgcgaaaagc ggcagatgtg
     2041 gcctcttcac atggtctgcc atcgggctcg acttgtggag cgcttcctgg aatggtcgca
     2101 ggccatcggc gtgatcgaca cgaatccgtt tgctgagttg catcgccact atgggccgcg
     2161 tacgacaccg attgtgcgcg cgctagtcag tgatgatgtc gaggcg
//
LOCUS       DATMGX010000474         4614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_64587, whole genome shotgun sequence.
ACCESSION   DATMGX010000474 DATMGX010000000
VERSION     DATMGX010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64587"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VNR65_07660"
     CDS             <1..216
                     /locus_tag="VNR65_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18585.1"
                     /translation="EDNQYQYRFRDIVDPASGTHLFTVEHEVRSATHPMYLRPLINRD
                     PAQTNRTYAHGHDQWPWAMGPDQFDGR"
     gene            694..975
                     /locus_tag="VNR65_07665"
     CDS             694..975
                     /locus_tag="VNR65_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18586.1"
                     /translation="MASSPLPDWMRPDKSGAIMLAANFFWRGLPVDVLVPAGEAPKKK
                     ALDWLMKFSTEKKRLLIYQMKEEWFAFGPPAFQAEISGRIARGEKPWAS"
     gene            1299..2936
                     /locus_tag="VNR65_07670"
     CDS             1299..2936
                     /locus_tag="VNR65_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWJ18587.1"
                     /translation="MRNKLLSGTACAALCLISFSGSARSATLDDVMARLDKIEKENAE
                     LRAKVKNLSAAKQAMVAPSAPVVTDPQKFKGNPVLHGAVATSPAPAPTTVIGGIPVKA
                     GPLTPLIDNTTVTLYGSIDLSGDIFNPSVFDQGTKLGIASNISSFGVRVRHNLAPYGW
                     EGMAAVAQLESQVDFASAPTERAAFGTRDSFLGLETPWGSIKAGKSDTPYKKSTAAMD
                     PFSRTLGDYNSIMGNTGGDNRAEFDWRMNHAVWYESPIFNGFQFSALASPGQNYAKDN
                     SDYSYGDTFQCNGASARGSGSNFPGTGGAVNGNIGGNGCTDGSFGNAYSAALTYKNGP
                     FSAIAAYELHEGVNRHGDDGLEPGLIAPVFLADGSQVLTGVHNEWAAKVGGGYHFNDG
                     LGDLQLNAFYEWIRREVTAIEQPFNERSRDAVFASATQTIGQWAISGSYAHAFKSPGN
                     PAMLSVNDPVLAPAATLQGNLFANDASQYAVGARYYFNNWASWYVVASQLNQGPGAHY
                     CLGASGHGYQVCSRDAANDTIGGATIRAATTGMTFNF"
     gene            3129..3449
                     /locus_tag="VNR65_07675"
     CDS             3129..3449
                     /locus_tag="VNR65_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18588.1"
                     /translation="MDMQAPPAFVQIVQADVPDAPVDPAPVEVNVSKYIPESATAVTI
                     IVTLTPTTGQAVIYSAGHENDGTLFKGARSIDEVRLSGPTIYVKLYGATSFDIQYINY
                     RQRE"
     gene            3483..>4614
                     /locus_tag="VNR65_07680"
     CDS             3483..>4614
                     /locus_tag="VNR65_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ18589.1"
                     /translation="MGHSTTEQLLKNAVAAQRRGALGDARQLYAAVLRIDPGNAAASG
                     NLAIIAAQQGDLATAERLIRREIELRPDYPANYNNLGSVLQQQARPADAIVAHRQAIK
                     LNPNYAEAYLALGNVLRQLDRHEEAMEAYRSAITARRDYPEAHNNIGVLLQAQGRLDQ
                     AASAYGDALALRPAYAEAQFNLGTVQHQMHELEAAEAAYRRVISLDPSIAVVHNNLGT
                     VLKDKGRLDAALTAFENAIRLNPDYAEAYYNRATVLQQQQRAEEALAAYGRAIELRND
                     YPDAINNAGIVLQELGRAGEAIDLYRRLLGQTPAHADACNNMGTSLLAEGRADEARVA
                     FEQALTHKPDFPEAFYNLGNALRERSDLSGAIAAYRNALRLRP"
BASE COUNT          977 a         1381 c         1361 g          895 t
ORIGIN      
        1 gaagataacc agtaccaata ccgctttcgc gatatcgtcg atcccgccag cggaacgcac
       61 cttttcacgg tcgagcacga ggtgcgcagc gccacccacc cgatgtacct gaggccgctg
      121 atcaaccgcg atccggcgca gaccaaccgc acttacgcgc acggccacga tcaatggccc
      181 tgggccatgg gaccggatca gttcgacggg cgatagccgc gtctgtcccg accggattga
      241 tcgtcgttcg ctatttgagt tcgcgtatcg aagacccgga cagctggaat gtttccgtcg
      301 cgggcttcgc catacgttcg gcaccgatgc ccggcgccgt caccgcgcgg caatcagggc
      361 tttgcgatta atttgatggc gctggcagat cgtcggctat gtcgggcggg acgcatttga
      421 tcatctggtg gaggcctaga tgagagttct attgcgttcg cgatgccatt gagcgagcat
      481 cgcctctcca tctgcggcac ctgtaatttg aatcagcaac acccgagaac ctgtgggaac
      541 tgcacggcat gctgcgatgg ctggctgcag atcgaagtgc gtggacacca cgtccgtccg
      601 gggaagccat gtccgttcag tgtcgggaat acgtgttcga tttacagcga gcgtccccag
      661 cacccctgcc gcgaattcat ttgcggatgg ctggtggcat caagtccgct gcctgattgg
      721 atgcgtccgg acaagtccgg cgcaatcatg cttgcggcga actttttctg gcgcggcttg
      781 ccggtcgacg tgctggtccc agccggagaa gcgccaaaga agaaagcgct cgactggctg
      841 atgaagttca gcacggaaaa gaagcgcttg ctgatctacc agatgaagga agaatggttt
      901 gctttcggtc cgccggcctt tcaggccgag atatccggcc gcatcgcgcg cggcgaaaag
      961 ccctgggcaa gctgaccgat tgatttcgcc ttcgcggcag cggaaaacaa ttgcgcaatg
     1021 cacaattttt gattgaaaaa tgctggcatc aagaggaagg ctccctctgc tcggcctcag
     1081 gtaagcccct tgaaaagcta tcaaatccgt ggctgttttc gccgaaatcc cccgtctcca
     1141 gtaagttgtt gcaacgcaac agtatttttg tgcctttttt gttacagatt acaattcttt
     1201 gacgtgcggc ccagcgaagc cttccttgtg gcatctggta taccttacgc tgccatcgcc
     1261 cataagttga aacgggtgca aagcggtggg gaaaagccat gaggaataag cttctttcgg
     1321 ggacggcgtg cgccgctctc tgcctgatct cgttcagcgg ttcagcacgg tctgcaacac
     1381 tcgacgatgt gatggcacgg ctcgacaaga tcgaaaaaga aaacgccgaa ctgcgagcga
     1441 aggtcaaaaa cctgtctgcc gcaaagcagg cgatggttgc tccctccgca ccggtcgtga
     1501 ctgatccaca gaaattcaag ggcaatcccg ttctgcacgg ggccgttgcg acgtcgccgg
     1561 cgccggcacc caccaccgtg attggcggca ttccagtaaa agcgggcccg ttgacgccgt
     1621 tgatcgataa cacgaccgtt acgctttatg gatcgatcga tttgtccgga gacattttca
     1681 atccgtccgt attcgatcag ggaaccaaac tcgggatcgc gagcaacatt tcgagtttcg
     1741 gcgtccgggt aaggcacaac ctcgcgccat acggctggga gggcatggcg gctgtggcgc
     1801 aattggaatc tcaggtcgat ttcgcgtcag ctccgaccga gcgtgcagcc tttggaacgc
     1861 gcgacagctt cctgggattg gagactccgt ggggctccat caaggccggc aagagcgaca
     1921 cgccctacaa gaaatccacc gcagccatgg atccgttctc ccgaaccctc ggcgattaca
     1981 attcgatcat gggcaacacg ggcggcgaca accgcgccga atttgattgg cgcatgaacc
     2041 acgcggtctg gtacgaatcg ccgatcttca acggcttcca gttcagcgcg ctggcttccc
     2101 ccggtcagaa ctacgccaag gacaacagtg actactccta tggcgacacg ttccaatgta
     2161 acggcgcgtc ggcccgcggc agcggcagca acttcccggg caccggcgga gcggttaacg
     2221 gaaatatcgg cggtaatggc tgtaccgacg gctccttcgg caatgcctat agcgcggctt
     2281 tgacctacaa gaacggaccg ttttcggcga tcgccgccta tgaactccat gaaggagtca
     2341 atcgtcatgg cgacgacgga ctcgaacctg gtctgatcgc tccggtcttc ctggccgacg
     2401 gttctcaggt cctcaccggc gttcataatg aatgggccgc gaaagtcggc ggcggctatc
     2461 acttcaacga cggcctcggc gatctccagc tgaacgcctt ctatgagtgg attcggcgcg
     2521 aagtgactgc gatcgagcag cccttcaacg aacgctcccg cgatgccgtg tttgcaagcg
     2581 ccactcagac gatcggccag tgggcaatca gcggctccta tgctcacgcc ttcaagagtc
     2641 cgggcaatcc cgccatgctg agcgtcaatg atccggtgct tgcgccggcc gcgaccctgc
     2701 aaggaaactt gttcgcgaac gatgccagtc agtacgcggt cggcgcccgt tactacttca
     2761 acaactgggc cagctggtac gtcgttgcct ctcagctgaa ccagggtccg ggcgcgcatt
     2821 actgtcttgg cgccagcggc catggctatc aggtctgctc tcgcgacgct gccaacgata
     2881 caatcggcgg cgcgacgatc agggctgcca cgaccggcat gacgttcaac ttctgagcct
     2941 ctggggatat gggataaagg gggactttga cgagtctccc tttttctcgc cgcccgagaa
     3001 ttttgcttgc ggccgagaat tctaatcgca cctgccgccg acaaataaaa tgcctaaaaa
     3061 cagcttttgc cgccgctccg cagagctgga atttggtaaa cttgcaggca atacagggag
     3121 gcagaccagt ggatatgcaa gccccgccgg cctttgtgca aattgtgcag gctgacgtcc
     3181 cggatgcacc ggtcgatccg gcgcccgtcg aggtcaatgt atccaaatac atcccggaat
     3241 cggcgaccgc ggtgacgatc atcgtgacgc ttacgccaac gaccggtcag gccgtcatct
     3301 attcagccgg ccacgagaat gacggcactt tgttcaaggg ggcgcgctcg atcgatgagg
     3361 tcaggctttc cggtccgacg atctatgtga agctttacgg agcaacgagc ttcgatatcc
     3421 agtacatcaa ttacaggcag cgcgagtgaa cgctgcgctc ttggctgaaa acgaacgacg
     3481 ctgtgggaca ttcgacgacc gagcagttat tgaagaatgc cgtggcggcg caacgcagag
     3541 gcgcgctcgg agatgccagg cagctttatg ctgcagtgtt gaggatcgat cccggcaacg
     3601 ccgccgcgtc tggcaacctt gccatcatag cggcgcagca gggcgacctc gcaaccgccg
     3661 agcgcctcat ccgtcgcgag atcgagctga ggccggatta tccggcaaat tacaataatc
     3721 tcggctcggt gctgcagcag caggccaggc cagcggacgc tatcgtcgct caccggcagg
     3781 ccataaaact caacccgaat tatgccgagg cgtatcttgc gctcggcaac gtactcagac
     3841 agctggacag gcacgaagag gcaatggagg cctaccggtc cgccattacc gcgaggcgcg
     3901 attatcccga ggcccacaac aacattggcg tgctgctgca ggcgcagggc aggctcgacc
     3961 aggcggcatc ggcatacggc gatgcgctag cgctgaggcc cgcttacgcg gaagcccagt
     4021 tcaacctcgg cacggtgcag catcagatgc acgagctgga agccgccgag gcggcgtatc
     4081 ggcgggtgat ctcgctcgat ccctcgatcg ccgtcgtcca caataatctg ggaaccgtct
     4141 tgaaggataa gggccggctc gacgccgcgc tgacggcctt tgagaatgcc atcaggctga
     4201 atccggacta cgcggaagcg tactacaatc gggcgaccgt gctgcagcag cagcaaagag
     4261 cggaggaagc gctggcggcc tacggccggg ctatcgagct tcgcaacgat tatcccgatg
     4321 cgatcaacaa tgccggcatc gtgcttcagg aactggggcg cgctggtgag gcgattgatc
     4381 tctaccgacg gctcctcgga caaacgccgg cgcacgccga tgcctgcaac aatatgggga
     4441 cctcgttact cgcggaaggg cgcgccgacg aagcgcgggt agcgttcgag caggcgctga
     4501 cacacaagcc cgattttccc gaggcatttt acaacctcgg aaatgccctg cgggaacgca
     4561 gcgatctctc gggcgcaatc gccgcctatc gaaacgcctt gcgtctcagg cctg
//
LOCUS       DATMGX010000475         2108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_6681,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000475 DATMGX010000000
VERSION     DATMGX010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6681"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..516)
                     /locus_tag="VNR65_07685"
     CDS             complement(178..516)
                     /locus_tag="VNR65_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18590.1"
                     /translation="MKIHCSIIAAAFSLASLGVVQAEGLKPIESQAVDLDDLSGVAYY
                     TVERDGFHVIATLARQGEDGAPVRVETVLAPGQSVTFSTPRGVGAGPAAVEISRWNDH
                     VLVHKTSLVN"
     gene            627..1664
                     /locus_tag="VNR65_07690"
     CDS             627..1664
                     /locus_tag="VNR65_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ18591.1"
                     /translation="MMAIDYANLARLDLNLLVAFDALLSERSVTRAAARIGIGQSAMS
                     HNLSRLRALFGDELLTRAADGMRPTPRALALIDPVRVALSQIEALVSREKTFDPRTAD
                     RVFRIGLPDSVEVLLGPALLACLCETAPGVRLRLHSIDSSQVLDNLDADRLDLAFGIG
                     SFAEGQTHHKQRPLFSETYLCMFNAARVGLSPPISLDDYVRLPHVLTSLRPGEHGPVD
                     DALLKLGLKRTIVLTTPRFIAVPFLVRGAPVITTMHARLARFFADALSLALSPPPVDL
                     PEISISLLWHASYDHDPAHMWLRQTVARLAVESARDHDQDKRFQAKQLAQNKPNEKPK
                     ATSRTPARRRI"
     gene            1921..>2108
                     /locus_tag="VNR65_07695"
     CDS             1921..>2108
                     /locus_tag="VNR65_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWJ18592.1"
                     /translation="MERQMPNGTVKWFNPAKGFGFIQPDSGGADIFVHISAVERAGLS
                     SFDEGQKVSFDEERDPKKG"
BASE COUNT          390 a          677 c          622 g          419 t
ORIGIN      
        1 ggctgctaga aacccgcaac cagtagattt cgatcctgtg cccgacatcg tccgtgttcg
       61 cgcccggatg cgcgccggtc agcatgtcgc agaatgattg acgttggcga cattggagcc
      121 aagcctcggt cgtctctcat ccctatcctt gcaacacgcc gaccggcgct gccgggctca
      181 gttcacgagg ctcgtcttgt gcacgagcac atggtcgttc caccggctta tctcgacggc
      241 ggcgggtccg gcgcccacgc cgcgaggcgt ggagaacgtg acgctctggc cgggcgcgag
      301 gacggtctcg acgcgcacgg gcgctccatc ctcgccctgc cgcgcgagtg tggcgataac
      361 gtggaagcca tcgcgctcca ccgtgtagta ggcgacgccg gacaaatcgt cgagatcaac
      421 ggcttggctc tcaatcggct ttagtccctc cgcctggacc acgccgagcg acgcgagcga
      481 aaatgcggcc gctatgatgc tgcagtgaat tttcatgatc atttccttca taatctggtt
      541 tggcttggcg ccgcgctcaa agcccgacat ccgccgttga cacagagatg atctccgcta
      601 catcattagt caaatagatc ggatcaatga tggccatcga ttatgctaat ttagcccgcc
      661 tcgacctcaa cctgctcgtc gccttcgacg ccctgctttc ggagcgcagc gtcacccgcg
      721 ccgcggcgcg catcggcatt gggcagtcgg cgatgagcca caacctttcg cgcttgcgcg
      781 cgctgtttgg cgacgaactc ctcacgcgcg ccgcggacgg catgcgcccg acgccccgcg
      841 ccctggcgct catcgaccct gtgcgggttg cgctctccca aatcgaggcg ctggtgtcgc
      901 gggaaaagac cttcgatccc cgcaccgccg atcgcgtgtt ccgcatagga ctgccggata
      961 gcgtcgaggt gctgcttggc cccgcgctcc tcgcctgcct ttgcgaaacg gcgcccggcg
     1021 tcaggctgcg gctgcacagc atcgactcgt cgcaggttct cgacaatctc gacgcggacc
     1081 gcctcgacct cgcattcggg atcggttcgt tcgcagaagg gcagacgcac cataagcaac
     1141 ggcctctatt cagcgaaacc tatttgtgca tgttcaacgc ggcgcgggtg gggctctcgc
     1201 cgccgatctc gctcgacgat tatgtgcggc tgccgcatgt gctcacgagc ttgcggccag
     1261 gcgagcatgg gccggtggac gacgcgcttt tgaagctcgg cctcaagcgc acgatcgtcc
     1321 tgaccacgcc gcgcttcatc gcggtgccgt tccttgtgcg cggcgcgccg gtgatcacga
     1381 ccatgcatgc acggctcgca cgcttctttg ccgacgcgct cagcctcgcc ctcagcccgc
     1441 cgccggtgga tttgccggaa atatccatct cgctgctgtg gcatgcctcc tacgatcacg
     1501 acccggcgca tatgtggcta cgccagaccg tcgcacgcct cgcggtcgaa tcggcgaggg
     1561 atcatgacca agacaagcgt tttcaagcga agcagcttgc ccaaaacaag cccaacgaaa
     1621 aacccaaggc gacatcacgg acgccggcgc gaaggaggat ttgacgatac gtgacgaggc
     1681 gccttgcgcc cattacggct tgacgccgac ccgcgccgtc gacgcctgtt atcatcgcca
     1741 gaccgatatg atgtcagccc cgcgcttggt ctgtcgacac cgatgttcaa gaaaagtcca
     1801 tatgcgagtc ccctgttgct ttatggattc aagcttgata tccaagcgca atgtagccta
     1861 aaagcatttg tcatttttgt agggcaaact cattggttgt ccggtgcttg acgcgcccga
     1921 ttggagagac agatgccgaa cggcacagtg aagtggttca accctgcaaa gggtttcggg
     1981 tttattcagc cggatagtgg cggcgccgac attttcgttc acataagcgc cgtcgagcgt
     2041 gcgggtctga gcagctttga tgaagggcaa aaggtgtcgt ttgacgaaga gcgcgaccca
     2101 aagaaggg
//
LOCUS       DATMGX010000476         3703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_40837, whole genome shotgun sequence.
ACCESSION   DATMGX010000476 DATMGX010000000
VERSION     DATMGX010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40837"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..757
                     /gene="ppa"
                     /locus_tag="VNR65_07700"
     CDS             119..757
                     /gene="ppa"
                     /locus_tag="VNR65_07700"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002317.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWJ18593.1"
                     /translation="MDALPATHEDVKICSFKEPTTMRIDQIAVGRQPPHDVNVIVEVP
                     IGGEPIKYEMDKAAGTLVVDRFLYTSMRYPGNYGFIPHTLSGDGDPCDVLICNTRAIV
                     PGAVISVRPVGVLLMEDNMGMDEKIVAVPSSHLTQRYDRCLNYSDLPAITLQQIEHFF
                     QHYKDLEPNKWTKIIGWGDAAKAKQMILDGVQNHSAAAAAQARAERDPKPHV"
     gene            1250..1762
                     /locus_tag="VNR65_07705"
     CDS             1250..1762
                     /locus_tag="VNR65_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWJ18594.1"
                     /translation="MSRQASIELLARARLCRLACAHEGQPYVTPMHCAYDVNHLYGFS
                     TLGQKITWMRANPLVCVLVDEIVSPQDWASVIVFGKYEELTDATEFMAHRQHAYNLLQ
                     THPIWWEPGYVETVLHGEARLLEFMYFRIHIEQISGHRGAPDTVGLADSGASGGTGRW
                     FRRILGRQSS"
     gene            complement(2100..2531)
                     /locus_tag="VNR65_07710"
     CDS             complement(2100..2531)
                     /locus_tag="VNR65_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18595.1"
                     /translation="MQSIQYTLQVLEAYEDEVAGAAYFDGLALAYPQQSAFFERCAAL
                     ERETATQLSALLQKYQLTPRAQTTLEERGALDARKDAGSDWKRLMQQSIKSYARYVTE
                     FKALEAMGPVEDQHILAALTAHEIQLIEWMQAETGSAATSG"
     gene            complement(2736..>3703)
                     /locus_tag="VNR65_07715"
     CDS             complement(2736..>3703)
                     /locus_tag="VNR65_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ18596.1"
                     /translation="LMGGLIIGYFHWSVIFFVNIPIGIIGLILVYRHLPNYRATRNYP
                     LDVIGLALFGSGISLLSYVLEVFGEHTLSGREIAAFLAISAALLATYGLHSLRHAHPL
                     LRLDMARIRTFRVAISGNLLTRLGIGGLPFLLPLLYQVGLGFTPIQSGLMIMPQALAA
                     MSLKPTLPRILRQFGYRRVLIANTVMLGAIVMLFATIDARTPLWAIALQAFVYGIFTS
                     TQYTSMNTLGYADVNQDQASGASTIASTAQQLAVSFGVASASLTVAFFIPDHAHANPA
                     QMIHGIHLALLLLGAWTIVSTLVFKELKSDDGQAISSHKAGLSGE"
BASE COUNT          796 a         1056 c         1092 g          759 t
ORIGIN      
        1 cgtagcctac agatcattct gggcacccgc tttataagga gagtacgcgc gccgctattc
       61 atgagcatca tgattatttc gaccgaaata atctgtcacc tgcgtgcggg tcggcacatt
      121 ggacgcactg cccgccaccc atgaagatgt caagatttgc agtttcaagg agccaacaac
      181 aatgcgcatc gatcaaatcg ccgtgggcag gcaacccccg catgacgtca acgtcatcgt
      241 cgaggtgccc atcggcggcg aacccatcaa atacgagatg gacaaggcgg ccggcaccct
      301 ggtggtcgat cggtttctgt acacgtcgat gcgctatccc ggcaactacg gtttcatccc
      361 gcacacgctt tctggcgacg gagatccatg cgacgtgctg atctgcaaca cgcgagccat
      421 tgtccccggt gccgttatca gcgtgcgacc cgtgggcgtg ctgctcatgg aggacaacat
      481 gggcatggac gagaaaatcg tcgccgtacc gtcctcgcac ctcacccagc gttacgaccg
      541 gtgcctcaac tacagcgatc tgccggccat cacgcttcag cagatcgagc attttttcca
      601 gcactacaag gatctcgagc ccaataagtg gaccaagatc atcggctggg gcgacgcggc
      661 gaaggcgaag cagatgattc tggacggagt acagaatcac agcgccgctg cggcggcgca
      721 ggcacgagca gagcgcgatc ccaagccgca cgtgtgaggg atggcttgat gcgacgggat
      781 gcgcgccaat atgcgtgttc aaccggaaga ttgatgcgct cacgacggcg gtatacctcg
      841 attagatcgc ggcgcacgat cgcggcactc taattctgtc cacccgattc gcgccctatt
      901 catttttgtg gctccattgc agagattcca ccgcctgctc caagcccagc gcgatcaaca
      961 cgccaaccac gatgacgccg atctcctaaa tccgcattga tcgagtcaaa actgcggcaa
     1021 ttacatagcg aggtctcgga cagcccggag agcattgcaa agctcaagcg agaaagattg
     1081 gacttatagt tcggatgttt tctgtgattt agtggccgtt cgtcggcatt gggtttttgg
     1141 gaatgaactg cgccaccgag gcgcggctcc atttctgcga attcttcaag ccgctgactg
     1201 aaacatagtc gtgtctggaa tcggaaataa tcatatgtgg atacaagaca tgagtcggca
     1261 agccagcatc gagttgctgg cgcgcgcaag gttgtgcaga ttggcctgtg cccatgaggg
     1321 ccaaccctac gtcacgccga tgcattgtgc gtatgacgtc aaccacctct acggattctc
     1381 aacgcttgga caaaagatta cctggatgcg cgcaaatccg ttggtttgcg tgctcgttga
     1441 cgaaatagtg agcccccagg actgggcgtc ggtgatcgtt tttggaaaat atgaagagtt
     1501 aaccgatgcc accgagttca tggcgcatcg gcagcacgca tacaatctgc tgcaaacgca
     1561 ccccatatgg tgggaaccag gttatgtgga gactgtactt cacggggaag cgcgtctttt
     1621 ggaatttatg tattttcgga ttcatatcga gcaaatcagt ggccaccgcg gcgccccgga
     1681 caccgtgggt ttggcggatt ctggggcgtc ggggggaact ggtcgctggt tccgtagaat
     1741 tctgggtcgg cagagcagtt aagcagcgcc cgttaaatga ttcgcggtcg cgcggtcgtt
     1801 tccatatgat ttggtgccgc gagctttggc cgctcgctgt ggttacggcc cacagccgcc
     1861 cgagctgctg cgaacggctg cttcgcagta agcatttcgg caccctcact gaccggagtt
     1921 gaccgatcag cgaccgattg caacgctgcg aaggcggtca agacgcacca ccggcagcaa
     1981 tcggaaatcg aaattggagc agtgaggcta ctccggcagg ttcatcttgc gctggaacga
     2041 cttgcaccaa agggtcgggc tcgaacgtct tgaagagcca tttcccttca tcaggccggt
     2101 caacccgagg ttgccgcgga tccggtctct gcctgcatcc attcaatcag ttggatttcg
     2161 tgtgcagtca acgccgcgag aatgtgctgg tcctccacgg gccccatggc ctcgagcgcc
     2221 ttgaattccg tcacataccg ggcatacgac ttgatggatt gctgcatcag cctcttccag
     2281 tcgctgccgg cgtccttgcg ggcgtcgagt gcgccgcgct cctcgagcgt ggtctgcgca
     2341 cgaggcgtca gctgatattt ctgcagcagt gcgcttagct gagttgccgt ttcccgttcg
     2401 agcgccgcgc atctctcaaa gaatgccgac tgctgagggt acgctagggc caaaccgtca
     2461 aagtaggcgg cgcccgctac ttcgtcttcg taggcctcga ggacttgcaa ggtgtattga
     2521 atgctttgca tccggaccag agtctaccgg ggcggtcgcc ttgaccgcca tgcgatgacc
     2581 gactgtgcgg cccgataacg gccttgtacg ggccggcgga tggtcgcggt gatgccgttc
     2641 gcgctatcat agcttggcgc aacctcaacc acgtccatgc cgaccacgcg atgagcgcgg
     2701 gctatgcgcc gcgggtctgt ttttcgactt tgcggtcact cgccggacag accggccttg
     2761 tggctgctga tggcctgtcc gtcatcggac ttcaattcct tgaataccag ggtcgagacg
     2821 atggtccacg caccgagcag cagaagcgcg agatgtatac cgtggatcat ttgcgccggg
     2881 ttcgcatgag catgatccgg tatgaagaag gcaacggtga gcgatgcgga ggccacgccg
     2941 aaactgaccg ccaattgctg ggcggtgctc gcgatggtgc tcgcgccgct ggcctggtcc
     3001 tgattcacgt cggcgtagcc aagagtgttc atgctggtgt actgcgtgga tgtgaaaatt
     3061 ccgtagacga aggcctgcag cgcgattgcc cacagcggcg tgcgggcatc gatggtcgcg
     3121 aacagcatca ctatggcccc gagcatcacg gtgttcgcga tcaacacgcg gcggtagccg
     3181 aactgcctca atatgcgcgg cagcgtgggc ttcaagctca ttgcggcgag cgcctgcggc
     3241 atgatcatca aaccggattg aatgggtgtg aatcccaaac ccacttgata gagcaacggc
     3301 agtaaaaagg gcagaccgcc gatccccagt cgcgtcagca aattgccgga tatcgccacc
     3361 cgaaaggtgc gaatacgcgc catatcgagc cgcagcagcg gatgggcatg tcgcagagaa
     3421 tgcaggccat aggtggcgag cagcgcggcg gatatcgcca ggaaagccgc gatctcgcgg
     3481 ccgctcaggg tgtgctcgcc gaatacctcg agtacataag atagcaacga tattcccgaa
     3541 ccgaacagtg ccagtccaat gacgtccaga gggtagttgc gcgtggctcg ataattcggc
     3601 aggtgccggt acaccagaat caatccgatg atcccgatcg gaatgttgac gaaaaaaatc
     3661 acgctccagt gaaaatagcc gatgatcagg ccgcccatca gcg
//
LOCUS       DATMGX010000477         2140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_41466, whole genome shotgun sequence.
ACCESSION   DATMGX010000477 DATMGX010000000
VERSION     DATMGX010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41466"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2140)
                     /locus_tag="VNR65_07720"
     CDS             complement(<1..>2140)
                     /locus_tag="VNR65_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWJ18597.1"
                     /translation="ALNHPNILAVFDIGTSEGSPYVVSELLEGETLRERLRSGAIAVR
                     KTLDYALQIAHGLAAAHEKGIIHRDLKPDNLFVTKDGRVKILDFGLAKLTQSDPGSGS
                     STSVPTATHHGTEAGVVMGTAGYMSPEQVRGMALDPRSDIFSFGAILYEMLSGKRAFH
                     GDTAADTMSSILKEDPPELTETNRNVSPALERIVQHCLEKNPESRFHAASDIAFDLEH
                     LSGLSGSAARIASAGSGASFAIDQPRHKLLIGLAAGLAFAGLVYGLGWWSGSAHSHAP
                     QAEYQQITFRTGSIGNARFTPDGSIVYSASWDGGDNQLYMSRTDDPGARELGIKDAEL
                     LSISKSGEMAIRLNTQFYAGYARSGTMARVPLSGGTPREVLDNVQDADWSANGESMAV
                     VRYVPENNHWRLEYPAGKVLIDSINWMSHPKISPDGKWIAFADHENTGGDDEGSVAVI
                     GADGKAAEKKLSSGWTSLQGILWSPASDEIWFTSTNTGSASNPRAVTLSGKLRTLANV
                     PGGMWLEDSRNGTVLSVANHTRLDIRGKGPDAKEERELGWFGWSELRDISRDGRKILF
                     EEEGDGGGANYTVFLRDTDGSPPARLGEGLAGAISPDGKWVVTMPTKVGAFSLVPTGA
                     GESRQITHDSVSYGVVRFLPDGKHLLAVGIEAGHGGRDYLIDVSSGESKPITPEGISG
                     VQISPDGKYTVVRGPGGQRGIWPLEGGGFRP"
BASE COUNT          408 a          711 c          575 g          446 t
ORIGIN      
        1 tggggcgaaa ccctccgcct tccagcggcc agatgcctcg ctgtccgccg ggcccgcgga
       61 ccaccgtgta cttgccatcc ggcgaaattt gcacaccgga gataccctcg ggagtgatgg
      121 gcttcgattc gccgctgctc acatcgatca ggtagtcgcg tccaccgtgc ccggcctcga
      181 ttcccaccgc cagcaaatgc ttgccatcgg gcaaaaaacg aactacgccg tagctgacag
      241 aatcgtgagt aatctgccgg gattcgcctg ctcccgtggg caccaggcta aacgcgccaa
      301 cttttgtcgg catcgtgacc acccatttgc cgtcgggaga gatcgctcct gccaggccct
      361 cgcccagacg cgccggaggc gacccatccg tatcacgcag gaagacggtg taattcgctc
      421 ccccgccatc gccttcttct tcaaacagaa tcttgcggcc gtcgcggctg atatcgcgga
      481 gttccgacca tccgaaccac cccagttccc gctcttcctt agcgtccggc cccttgccgc
      541 ggatatcaag gcgggtgtga ttggccacgc tcagcacagt tccattgcgc gaatcttcca
      601 gccacatgcc gccgggcacg ttagcgagcg tccgtagttt gcccgaaagg gttacggcac
      661 gcgggttcga ggcgctgccg gtattggtgg aggtgaacca tatctcatcg ctcgccggcg
      721 accacaagat cccttggagt gaagtccagc cagaagaaag tttcttttcc gctgctttgc
      781 catcggcccc gattacagca actgaacctt cgtcgtcgcc gccggtgttt tcatgatcgg
      841 caaaggcgat ccactttccg tccggtgaaa tcttcggatg gctcatccag ttgatgctgt
      901 cgatcagcac cttgcccgcc gggtattcca gacgccagtg gttattttcc ggaacgtaac
      961 ggaccaccgc catactctcg ccattcgccg accagtccgc gtcctgtaca ttgtccagaa
     1021 cttctcgcgg cgttcctccg ctcagcggaa cccgcgccat cgtcccgctg cgggcgtagc
     1081 cggcgtaaaa ctgtgtattg aggcgaatcg ccatttctcc gctcttggag atcgaaagca
     1141 gttccgcatc cttgatcccc aattcgcgcg cgcccggatc gtccgtgcgc gacatataaa
     1201 gctggttgtc gccgccgtcc cacgatgcgc tgtaaacaat gctgccgtcc ggcgtaaatc
     1261 gcgcgttacc gatcgaaccc gtgcgaaacg taatttgctg atactcggcc tgaggcgcat
     1321 gactgtgcgc gcttccgctc caccacccga gaccataaac cagacccgca aacgcgagcc
     1381 cagccgcgag cccaatcaga agtttgtggc gtggttggtc aatagcaaag ctcgcgccac
     1441 tgcccgccga cgctattctc gccgcgctcc cggaaagccc agacagatgt tccagatcaa
     1501 acgcaatgtc gctggcggca tgaaaacgcg actcgggatt cttctccagg cagtgctgca
     1561 caatgcgctc cagcgccggt gaaacattgc gattcgtttc agtcagctcc ggaggatctt
     1621 ccttcaggat cgagctcatc gtgtccgcgg cagtgtctcc atggaatgcc cgtttgcccg
     1681 agagcatctc gtagagaatt gccccaaagc tgaatatgtc ggagcgcgga tcgagcgcca
     1741 tgccccgcac ctgctccggc gacatatatc ccgccgtccc catcaccacg cccgcctcgg
     1801 tgccgtgatg agtcgcggtt ggcactgaag tgctcgatcc cgagccggga tcggattgag
     1861 tcagcttcgc cagtccgaag tcgaggatct tcactcgccc atctttagtg acgaataaat
     1921 tgtccggctt gagatcgcga tgaataattc ctttttcgtg ggccgcggcc agtccatgcg
     1981 cgatttgcaa cgcgtagtcc agagtcttcc gtaccgcgat cgctccgctc cgcagtcgct
     2041 cccgcagtgt ctccccctcc agcagttcgg acaccacgta aggcgagccc tcgctcgtgc
     2101 caatgtcgaa tacggcaaga atgttgggat gattcagcgc
//
LOCUS       DATMGX010000478         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_306718, whole genome shotgun sequence.
ACCESSION   DATMGX010000478 DATMGX010000000
VERSION     DATMGX010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306718"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VNR65_07725"
     CDS             <1..334
                     /locus_tag="VNR65_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18598.1"
                     /translation="WFASMPEALTQLEAYLRRTASVEASPPPQGYSVAVCGERPAFEK
                     TVTLPQLRWDFKPEWDQSDFFIAPTHMNCDSDLDGKVIGTVERLGVTIAYIKDRRALI
                     RPVKAAAR"
     gene            331..1497
                     /locus_tag="VNR65_07730"
     CDS             331..1497
                     /locus_tag="VNR65_07730"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HWJ18599.1"
                     /translation="MNIAAQEPAQATVLKPATPLPANERELRIDLFRGLALWLIFIDH
                     LSPDVLSWFTIRTYGFSDAAEIFIFISGYTAALVYGRAMFEFGFLVATARIMRRVWQI
                     YLAHVFLFTVFLAEIYFLATRVESAFYSEEMGVVEFFKQPGETLVQALLLRFRPLNMD
                     VLPLYIVLMAFLPLILWLIKLRADIALALSVMLYAITWRFDLHLTAYPNGFWSFNPFA
                     WQLLFVFGAWCALGGGRRMSRVLSSPITLWVSIAYVFLAFCVTMTWYFPQLDHVLPRR
                     IEQWMYPIDKTDLDVLRFAHFLALAAITVHFVPKGWPALKSPWLRPLILCGQHSLEIF
                     CLGVFLAFAGYFLLAEISGGIGLHFLVAIIGLLIMSAVAWLTSWHKFSHKGRRG"
     gene            complement(1805..1933)
                     /locus_tag="VNR65_07735"
     CDS             complement(1805..1933)
                     /locus_tag="VNR65_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18600.1"
                     /translation="MGKDKKALLRLWREKRGEAAALDLSGVLIVRPGLVTEDLNRR"
BASE COUNT          371 a          576 c          598 g          500 t
ORIGIN      
        1 ttggttcgcc agcatgcccg aggcgctcac tcagctcgag gcctatctgc gccgcaccgc
       61 gtccgtcgag gcgagcccac caccccaggg ctattccgtc gcagtgtgcg gagaacgacc
      121 tgcgttcgag aaaactgtta cgcttccgca actgcgatgg gacttcaagc cggagtggga
      181 ccagtcggac ttcttcatcg cgccaactca catgaattgc gacagcgatc ttgacggcaa
      241 ggtaatcggc accgtcgaac ggcttggcgt taccatcgcg tatatcaagg accgtcgggc
      301 actgattcgg ccggtaaaag cggccgcacg gtgaacattg ccgcacaaga acccgcgcag
      361 gcaaccgtct tgaagccggc tactccgctt cccgcgaacg aacgggaatt gcggatcgat
      421 ctgttccgtg gtctggccct gtggctgatc ttcatcgacc atctatcgcc cgatgtcctg
      481 agctggttca caatccgcac ttacggattc agcgacgcag ccgagatctt catctttata
      541 tcgggctaca cggcggcgct ggtttacggt cgcgcgatgt ttgaattcgg tttcctggtc
      601 gcgaccgcac gcatcatgag gagggtttgg caaatctacc tcgcgcatgt gtttctgttc
      661 acggtctttc tggcggaaat ctacttcctc gccacccggg tcgaaagcgc cttttattcc
      721 gaggaaatgg gggtcgtaga gtttttcaag caacctggcg agaccctcgt tcaagctttg
      781 ctattaagat ttcgtccgct gaacatggac gtcctgccgc tctatatcgt gctgatggct
      841 tttttgccgt tgatcctgtg gctgatcaag ttgagggcag acatcgcatt ggcgctgtcg
      901 gtcatgcttt atgcaatcac ctggcggttc gatctgcatc tgacggccta tccgaacggc
      961 ttttggtcct tcaatccgtt tgcctggcaa ttattattcg tgttcggcgc gtggtgcgca
     1021 ctgggcggcg gccgaagaat gtcgcgggtt ctctcgtcac cgatcacgct gtgggtatcg
     1081 atcgcatatg tgttcttggc gttctgcgtc acgatgacgt ggtattttcc gcagctggat
     1141 catgtgctgc cacgtcggat cgagcaatgg atgtatccca tcgacaagac cgatctggac
     1201 gtgctccggt tcgcgcactt tctggcgctg gcggcgatca cggtgcactt cgttccgaag
     1261 ggctggcccg cattgaaatc tccatggctg cggccgctga tcctctgcgg gcagcactcg
     1321 ctggagatat tctgcctcgg cgttttttta gcgttcgctg ggtacttcct gttggctgag
     1381 atctccggcg gcattggcct gcatttcctg gtcgctatta tcggactcct gatcatgtcc
     1441 gcggtggcct ggctgacttc atggcacaaa ttctcgcaca agggtcggcg tggatgactg
     1501 acggcaaggg ggatgttgca cgagcgcata gtctgtttgc gacgatcctt gagtccggtt
     1561 gggccttttt tcgacgcagc ttttcggtga gcgtctttgt tacgccaaag agacagggat
     1621 tcgacgatca gatgctttgc aaacggtagc aacaattgga aggtctccac ggcgccatga
     1681 acttggcttc gaagacggcg ccggtttcct cgaccagcca tccagcgtcg cggccatcca
     1741 agggatcgtc cggtggacgg tatgccgttg gacgtcgccg atccggtgac cggagttaac
     1801 ctcgttagcg gcggttgagg tcttcggtta ccaagccggg ccgaacgatc aggacgcctg
     1861 aaaggtcgag tgcggctgcc tcacctcgct tttcacgcca caggcgcagg agggcctttt
     1921 tgtccttgcc catgatcacc cacgcgatgc agaatgcttt agaaagggcc ggaattcagt
     1981 tcattgctaa agacggtgga agcccgggag tgcggttgca aaacggatac actcgtcgga
     2041 catcc
//
LOCUS       DATMGX010000479         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_268790, whole genome shotgun sequence.
ACCESSION   DATMGX010000479 DATMGX010000000
VERSION     DATMGX010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268790"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..907)
                     /gene="flgF"
                     /locus_tag="VNR65_07740"
     CDS             complement(188..907)
                     /gene="flgF"
                     /locus_tag="VNR65_07740"
                     /inference="COORDINATES: protein motif:HMM:NF009282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HWJ18601.1"
                     /translation="MQSSLYVGLSAQVALQNRLDTIAQNVANGTSAGYRGSEMKFNAV
                     MSQMTAPQVSFVSSGSGVIKRGSGQFVKTGNPLDVAVRGDGWLSIQTPGGQAYTRDGR
                     MRMGTAGDLVSLTGAPILDAGGGAIQLDPTAGQPHIAEDGAITQGQSRVGVLGLFTLP
                     TNAQLSRAEGNAFTSNIPGTPIVDSAANGVVQGFSEQSNVNPMTELTRLIYVQRAFDG
                     VTTTLHATESSLKNAIQTLGA"
     gene            complement(977..1363)
                     /locus_tag="VNR65_07745"
     CDS             complement(977..1363)
                     /locus_tag="VNR65_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ18602.1"
                     /translation="MDPNLQILVVDDYNTMVRIVRKLLKTIGYENVDEASNGEMALGM
                     IKQKSYGLIISDWNMEPMTGYELLKHVRAAPETAKTPFILVTAESRPDNVVAAQKEGA
                     NEYLVKPFSAPVLKEKIDKALATVAA"
     gene            complement(1453..>2175)
                     /locus_tag="VNR65_07750"
     CDS             complement(1453..>2175)
                     /locus_tag="VNR65_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWJ18603.1"
                     /translation="VRAEADRSKEVHKATRGNQRIGMEIRGQDMGGLKTLAAAVLFTF
                     SAIASAVAEPDLCEREMTQAAKKYDIPLGILFAVGLTETGIGGHLHAHALNLQGDTVY
                     SLNKQQAMQRFQAAKASGVRLIDVGCMQLNYFYHGEHFSSVEEMFDPHKNVDYAARFL
                     KGLRQSEGSWTLAVGRYNAGKNNNPAQKRYVCRVLAHLAASGFGAWTSRSRAFCGNEA
                     NADKAATPKALGVWDASSVQAH"
BASE COUNT          429 a          642 c          595 g          509 t
ORIGIN      
        1 tccgccaagg ctctcagcgt attccgcccc ccatcgccgg gtggaatctc ttcaagcgtc
       61 atcagagtgc gttcggaaaa gtgtgaagcg gttttccgat gaagatgcac gacaaaacaa
      121 aaacttagag cccgcttttg attccatcaa aagcggatag gctccagctt ggcgcccgca
      181 aatcaaatca agctccaagc gtctggatgg cgttcttgag gctgctttcg gtcgcgtgca
      241 gcgttgtcgt gacgccatcg aacgctctct gcacgtagat cagacgcgtc agttcggtca
      301 tggggttcac gttcgactgc tcgctgaagc cttgcacgac accgttcgcg gcggagtcca
      361 caatcggcgt tccagggatg ttcgatgtaa aggcattgcc ctccgctcgc gagagttgag
      421 cgttggtcgg gagcgtgaac aggcccagaa ctccgaccct gctctgtccc tgagtgattg
      481 ctccgtcctc agcgatgtgg ggctggcctg cagtcgggtc caactggatc gccccgccgc
      541 ctgcatcgag gatcggagcg ccagtcaagg agacgagatc gcccgccgta cccatgcgca
      601 tgcggccgtc ccgtgtatag gcctggccgc ccggggtctg gattgacagc cagccgtctc
      661 ctctgaccgc gacatcgaga ggattgccgg ttttgacgaa ttgtccggag ccgcgcttta
      721 tgacgccgct gcccgaagag acgaaggaaa cctgcggggc ggtcatctgc gacatcacgg
      781 cgttgaactt catctcgctc ccgcgatagc cggcggacgt tccgtttgcg acgttctggg
      841 cgatggtatc aaggcgattc tgaagagcga cctgcgcaga aagcccgacg tataagctcg
      901 actgcatcgt gttcccaatc aatccgttcg aagataacgc atgacctttg tcgaaaagct
      961 gtgtggcggt cagggcctat gcggccacgg tagcaagcgc cttatcgatc ttctccttga
     1021 gcactggcgc gctgaacggc ttcacgagat actcgtttgc gccttccttc tgggcagcga
     1081 ccacattatc cggcctcgac tccgcagtga ccagaatgaa gggcgttttt gcggtctccg
     1141 gggcggcccg gacatgcttt agcaactcat agccggtcat cggctccata ttccagtcgg
     1201 agataatgag cccatagctc ttttgtttta tcatacccag ggccatctcg ccgttcgacg
     1261 cttcatcgac attctcgtag ccgattgtct tcaatagctt ccgaacgatc ctgaccatcg
     1321 tattgtagtc atcaacgaca agaatctgca gatttggatc catgatttaa ctccctcaat
     1381 agagctggct tcttgggcgt gatctcagac gcagctcaac ttcttttgac gatatcctgc
     1441 gcctacgttc acttagtgag cttgcacgga actggcgtcc cagacaccta aagctttcgg
     1501 agttgccgct ttgtccgcgt ttgcctcgtt gccacagaac gccctgctac gggacgtcca
     1561 ggctccgaag ccgcttgcgg caagatgtgc caggactctg cacacatagc gcttttgcgc
     1621 cggattgttg ttcttgcctg cgttgtaccg gccgacggca agagtccagc ttccttcgct
     1681 ttgcctgagc cctttcaaga agcgggcagc atagtcaacg ttcttatgag gatcgaacat
     1741 ctcctcgacg gacgagaagt gctctccgtg atagaagtaa ttcagctgca tgcagcccac
     1801 atcgatcaat cgcacgccgg aagccttggc tgcttgaaat ctttgcattg cctgctgctt
     1861 attgagagag tagacggtgt ctccctgaag attgagggca tgggcatgca gatggccccc
     1921 aatcccggtt tcggtcaaac caacagcgaa gagaatgccg agcggaatgt cgtacttctt
     1981 ggctgcctgc gtcatctcgc gctcacagag atcgggctca gcaactgcgg aggcgatggc
     2041 gctaaacgta aagagaaccg ccgccgctaa ggttttcagt ccgcccatgt cctggcctct
     2101 gatctccatt ccgatccgtt ggttgcccct tgtggctttg tgaacttcct tgctcctgtc
     2161 cgcttccgct cgaac
//
LOCUS       DATMGX010000480         2390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_228821, whole genome shotgun sequence.
ACCESSION   DATMGX010000480 DATMGX010000000
VERSION     DATMGX010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228821"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VNR65_07755"
     CDS             complement(<1..981)
                     /locus_tag="VNR65_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWJ18604.1"
                     /translation="MRPVYLDNNATTRMDPAVLAAMLPYFTDHFGNASSSHGFGAESG
                     AAIKAAREQLQALIGAAHSHEIVFTSGGTESDNAAILSALEAEPARKEVIVSAVEHPA
                     ILAFAMHLQRDYGIVVHVIPVDDKGRIDVDAYKAALGYKTAIASLMWANNETGTIFPV
                     AELAELAHEAGALFHTDAVQAVGRIPIDLKATEIDMLSLSAHKCNGPKGIGALYIRKG
                     MRFNPQIRGGKQERGRRAGTENTPGIVGLGQAAELAHQRLASNAGQRVRNLRDWFEAQ
                     VLHRIGHCYLAGDVDNRLPNTSNIVFERAEGEVILSGLNKAGIAASSGSAC"
     gene            complement(1013..1237)
                     /locus_tag="VNR65_07760"
     CDS             complement(1013..1237)
                     /locus_tag="VNR65_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HWJ18605.1"
                     /translation="MIRAALDEIRPYLQRDGGDCELVGVEGNVVRVKMKGACIGCQLA
                     SVTIHGIQAKLIAKLGMQLRVAPVLGFGGL"
     gene            complement(1332..1652)
                     /locus_tag="VNR65_07765"
     CDS             complement(1332..1652)
                     /locus_tag="VNR65_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HWJ18606.1"
                     /translation="MINLTENAISAVRTAISRADAPIEGLRLAVDAGGCAGFKYMMGL
                     VTKADPDDVVVERDGVKVFVDPDSVMYLDGTTVDFVIGIQNSGFTFENPQATSKCSCG
                     KSFG"
BASE COUNT          506 a          713 c          669 g          502 t
ORIGIN      
        1 gcaggccgac ccggacgatg cggcgatgcc agccttgttg agcccggaga gaatcacttc
       61 gccctctgcg cgttcgaaga cgatgtttga cgtattcggc agcctattat cgacgtcgcc
      121 cgccaggtaa cagtggccga tacgatgaag gacttgagcc tcgaaccagt cgcgcaagtt
      181 gcgcacgcgc tgccccgcgt tggacgcgag ccgctgatga gccagttccg ctgcctggcc
      241 cagaccgaca atgcccggcg tgttctcggt tccggcgcgc cgtccgcgct cctgttttcc
      301 gccccggatc tgcggattga accgcatgcc cttcctgata tagagcgcgc cgatcccctt
      361 gggcccattg cacttgtgtg ccgaaagcga cagcatgtcg atttccgtgg ccttgaggtc
      421 gatcgggatt cggcctacgg cctggacggc gtccgtgtga aagagtgcac cggcttcgtg
      481 cgcaagctcg gcgagttccg cgaccggaaa gatcgtccct gtctcgttgt tagcccacat
      541 gagggaggca atggccgtct tgtagccgag cgccgccttg taagcgtcga cgtcgattct
      601 gcccttatcg tcgaccggaa taacgtgaac gacgataccg taatctcttt gcagatgcat
      661 cgcgaacgcc aggatcgccg ggtgctcgac ggcggaaaca atcacttcct tgcgggcggg
      721 ttccgcctca agcgccgaga ggatcgccgc attgtcggac tctgtgccgc cggaggtgaa
      781 gacaatctcg tggctatgtg cggcgccgat cagcgcctga agctgctctc gggcggcctt
      841 gatcgcagcg ccgctttccg caccgaagcc gtgcgacgac gacgcattgc cgaagtgatc
      901 cgtgaagtaa ggtagcatgg ccgccaggac cgcagggtcc atacgcgtcg tcgcattgtt
      961 atccagatac acgggccgca taacgccctc cgatctcaac accttacaag tcttacaggc
     1021 ccccgaaacc gagaacgggc gcgacacgaa gctgcatccc gagcttggca ataagctttg
     1081 cctgaatgcc atggatggtg acggaagcga gctggcagcc gatgcaagcg cccttcatct
     1141 tcactctcac cacgttccct tccacgccga ccagctcgca atccccgccg tcgcgctgca
     1201 gataagggcg tatttcatcg agagccgcgc ggatcatcgc gaccctagtc tcctccgcgg
     1261 tttccaggag ggggtccgac gggagttcgc taatcttttc ctgtatttcc aacatggccg
     1321 cgtctccttg ctcaaccaaa tgacttgccg cacgagcatt tcgacgtcgc ctgcgggttc
     1381 tcgaaggtga agccggaatt ctgaatgccg atcacgaaat ctaccgtcgt gccgtcgaga
     1441 tacatcacgc tgtccgggtc gacgaaaacc ttcacgccgt cgcgctcgac gacgacgtca
     1501 tcgggatcgg ctttcgtgac gagccccatc atgtatttga agcccgcgca gcccccggca
     1561 tccaccgcga gccgcaaacc ttcgatgggc gcatctgcgc gagagattgc ggtgcgaacg
     1621 gcgctgatgg cgttttccgt gagattgatc attcaagacg gctttctgct cgttaagggc
     1681 gtctgatttc ccttgagcaa tagctgtgcc atccgcataa gccattgaat tataagagca
     1741 ttatcgctca ttggccggtt ctctcgatgg aatgtcgggt ttgcgacacg tctgtttcct
     1801 gacgcgcgcg gggaacaatc aggccgagca gaccttcgcc aatcgcatgc gcaggtgaat
     1861 tcgatgccgc ccgctggccg aatcttgcgg catgatgaca cacaaacatc acttctgcga
     1921 caaccagaac cggcgtacgt tgtgaccgca gtgcaataaa ggtgataaac ggaagagcgt
     1981 gcgtcaggga tgcgctgcgg catcggagag caaccatgcg ggtatcaaag agaaatcttg
     2041 gacgaaatat ctaacacctt gaatattgtg cggcgcaaca aaacacggcc gcgcgtgcga
     2101 aggtttactt cgcttttact tcccattaaa aagcacttaa cttcgttttt gatctaggtc
     2161 aaggtgaagt tttttaagtc tgagtacggt atccacgcga gaaactttgg aaaaagaaag
     2221 aggattggcg tatcaaaccg tcagttcgca cctaacaagg caagctttcc tttgaccaaa
     2281 gacagcaacg ggggcgcata tccctgcgcc ttttcacttt attagctaca gaccgtgagc
     2341 acaaatgaag ttgagctact accccggctg caccatgaag aaccacgccg
//
LOCUS       DATMGX010000481         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_461272, whole genome shotgun sequence.
ACCESSION   DATMGX010000481 DATMGX010000000
VERSION     DATMGX010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461272"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..387
                     /locus_tag="VNR65_07770"
     CDS             139..387
                     /locus_tag="VNR65_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HWJ18607.1"
                     /translation="MTVHVTSGGQARTTDTVAFGSTVTFRREDRRVQNYRILGEDEAD
                     PKAGSISFVSPVGRFLIGKAVGDVVGPSGQALEIIEIS"
     gene            complement(601..762)
                     /locus_tag="VNR65_07775"
     CDS             complement(601..762)
                     /locus_tag="VNR65_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18608.1"
                     /translation="MLVRFRQELKRCHGARALIYSLQESKQALQERPLLADIVEKLEN
                     REAPKISQM"
     gene            824..1324
                     /locus_tag="VNR65_07780"
     CDS             824..1324
                     /locus_tag="VNR65_07780"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWJ18609.1"
                     /translation="MFDVSKKDNIDRVWDIIEKVSVCMLTTQFVGGLRARPLEARPDR
                     DAGLIFFVTDIHSAKEDEIEATPDVGLVFIDSNDKAYLSITGRACVVRDAEKTKAAWR
                     KTDEVWWPGGPNDPDVCLLRIEPFTAELWDGPAIAAVTAFEFAKARLTGEEPNLGENR
                     KVTVKM"
     gene            1368..1880
                     /locus_tag="VNR65_07785"
     CDS             1368..1880
                     /locus_tag="VNR65_07785"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HWJ18610.1"
                     /translation="MDLFVVPTIGFDLLYAFVIVRLDRRDLVWINVTTNPTEEWIARQ
                     ITEAFPWNEAPRHIIRDRDRIYGAVVSRRLRAMGIRDKPTAPASPWQNDFAERLIGSI
                     RRECLDHILVFGEAHLRRILRSYACYYNDIRTHRSLDKDAPTSRPVQRIGIIISRAIL
                     GGLHHHYVRI"
     gene            1914..>2133
                     /locus_tag="VNR65_07790"
     CDS             1914..>2133
                     /locus_tag="VNR65_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18611.1"
                     /translation="MHVEDLAKRNVEFFAGVARQASAPPGNMPIGSDKNRPGVIGTGK
                     ISPRALRIFAVRIWSATYDQTPYSERPMT"
BASE COUNT          452 a          595 c          609 g          477 t
ORIGIN      
        1 tcggcaagct gagcgcagtt ctccgcattc tctcggaagt ggtccgattg tttcatggcg
       61 tcctcctttg caggggacat agggcacagg aggccgccta caacgagcgc ctcggtgaca
      121 ctttctccat gctgattaat gactgttcat gtaacgtcgg gtggtcaagc taggacaacc
      181 gacactgtgg cctttgggag cacggtgacc ttcaggcgcg aggataggcg cgtgcagaat
      241 tatcgcatcc tgggagagga cgaagcggac cccaaggccg gttcgatttc cttcgtatcc
      301 ccagtgggaa ggtttctgat cggcaaagct gttggggatg tcgtaggtcc atccggtcaa
      361 gcgcttgaga tcattgagat ctcgtagcac gcccgcgccg gacgcagacg atgtttggaa
      421 tggccgcttt gcgccgattg tgttgaaaaa gtcctttttc gcttgtgact aaaaattctc
      481 agggccgctg gtgcgtctct ctcgttacga ggtgaggggc cacatcaatt accgcaaaat
      541 cgatcgctgg cgctcgtatt gattctacaa agccttgcag cagctgaaat cgccaatgcg
      601 ctacatttgc gagattttcg gagcctcgcg attttcgagt ttttcaacaa tatccgccag
      661 aagaggtcgt tcctgtaacg cctgttttga ttcctgcaga gagtaaatga gggcgcgcgc
      721 cccatggcag cgcttgagtt cttgccgaaa gcgcaccagc atgcgccgtt gcggccggaa
      781 ctcgcgcctt gtggatttga aaatcaggat gtagctctgt atagtgtttg acgtgagcaa
      841 aaaagacaac atcgaccgcg tctgggacat catcgaaaaa gtcagcgtat gcatgctgac
      901 gacgcaattc gtcggtggac tgcgtgcgcg accgctcgaa gcaaggcccg accgcgatgc
      961 tggcctgatt tttttcgtaa ccgatattca cagcgccaag gaggatgaga tcgaggccac
     1021 gcccgatgtc ggccttgtct ttatcgattc gaacgacaaa gcctatctat cgatcaccgg
     1081 ccgcgcctgc gtcgttcgcg atgctgaaaa gacaaaggcg gcctggcgaa agaccgatga
     1141 ggtgtggtgg ccgggcggcc cgaacgatcc cgacgtatgc ttgttgcgga tcgagccctt
     1201 cacggcggaa ttgtgggatg gaccggcgat cgctgccgtt actgccttcg agttcgccaa
     1261 ggccaggttg accggcgaag agccgaatct cggcgagaac cgcaaggtga cggtcaagat
     1321 gtgagcgtgc gaccccgcat gtcgccgatc tgccggacat tgccgttatg gatttgttcg
     1381 ttgtcccgac catcggcttc gacctgctct atgctttcgt catcgtccgc ctcgaccgca
     1441 gagaccttgt ctggatcaat gtcacaacaa acccgacaga ggagtggatt gcccgtcaaa
     1501 taacggaagc tttcccctgg aacgaggctc ctcgccacat catccgcgac cgggatcgca
     1561 tctatggtgc agtcgtctca cgtcgattgc gcgccatggg catccgggac aagccaaccg
     1621 cgccagcctc gccttggcag aatgactttg ctgaacggct gatcgggtcg atccgccgcg
     1681 agtgcctgga tcacatccta gtttttggtg aggcgcactt gcgccgaatc ctgcgatcct
     1741 atgcttgtta ctacaacgat atcagaacac atcggtcact ggataaagat gcgccgacct
     1801 ctcgcccggt tcagcggatt ggaatcatca tttcacgcgc gatactcggc ggacttcatc
     1861 atcattacgt ccggatttag gttttcgata cacacaggtc acacacctga cggatgcatg
     1921 tcgaggatct ggcgaagcgc aacgtcgagt tcttcgccgg ggttgcgagg caagcttcgg
     1981 ctcctccagg caacatgccg atcgggtcgg acaagaaccg ccccggagtc atcggtaccg
     2041 gaaaaattag tccacgcgcg ttgaggatct tcgcagtccg catctggtcc gctacctatg
     2101 accagacacc ttacagcgag cggcccatga cta
//
LOCUS       DATMGX010000482         2881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_47262, whole genome shotgun sequence.
ACCESSION   DATMGX010000482 DATMGX010000000
VERSION     DATMGX010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47262"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VNR65_07795"
     CDS             <1..1533
                     /locus_tag="VNR65_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18612.1"
                     /translation="WGPGSSEWNADWLSMTENMSQVYIGALPILVFLAVGFLRGGIFE
                     REVRFFAIAATGLFLFALGRYTGFFTLAYNHFPGVDLFRRPADATYALGGMASILSGY
                     LLHRFLSGSAPTSRQKRGVGVILALIFAVSLAVAAAHSHFYQAVKPVLLSIGIFTAGW
                     LSLVFARQYSNRYAVLTIAVLAAFTTADLAINNGPNRSTAQQPSYYDQMRPDTKNETV
                     AFIKTNLVQPLNSPRRDRVEMIGLGFEWPNISLIHGFDHALGYNPLRLAEIAEGGMGA
                     SETVAVDYQREFTPLYPSYRSMMADLLGLRYIVTDHPVEQIDKGLKPGDLTLVANTRD
                     AYIYENPRALPRVMFAFDWQPANFERITKDGHWPYFDPRQIVLLRGRQQLMSTLQPRP
                     VSLSQAATALQVYGNTEVQVVVDTPRAGFLVLNDVWHPWWFGTVDGKPADIFRANVLF
                     RAIEVPAGRHVVRFEFKPVEGAVKEIMAYLTGKPNEPTPPPWEPWLKPEASAPAFLRG
                     AG"
     gene            complement(1487..2290)
                     /locus_tag="VNR65_07800"
     CDS             complement(1487..2290)
                     /locus_tag="VNR65_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWJ18613.1"
                     /translation="MDEGVIINADDYAMDACVDSAILKLAAEGVVTATSAMVLSPTWP
                     EAAGPLADAPLSRGLHLDFTSPFAGDIFPRQTITGLTMRTHSGVLNRKLLRKEIDRQL
                     SQFEASMKALPDFVDGHQHCHLLPFIREALIDALADRYGDDARRISLRNCAPRRWRGL
                     KAAIIARTGTFRLEDLAREHGHGMNSDFAGVYDFNEDADLEALWTDWLQGLQGASPLV
                     MCHVAERGDHDGSDSIRGARTLEYDWLASSKFRELTRRLSKRPERWPQA"
     gene            complement(2668..>2881)
                     /locus_tag="VNR65_07805"
     CDS             complement(2668..>2881)
                     /locus_tag="VNR65_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18614.1"
                     /translation="RSLGKRFVAAPSVHTNVVNASAPDLFPLTIVLNKHFIPAAITSR
                     RSLVPVGSNSAMSLKPKSFHPLMTSH"
BASE COUNT          602 a          840 c          806 g          633 t
ORIGIN      
        1 tggggcccgg gaagctcaga gtggaacgcc gactggctct cgatgaccga aaatatgagc
       61 caggtctata tcggcgcgct tcccatcctt gtttttctcg cggtgggctt cttaagaggc
      121 ggcatctttg agcgtgaggt ccggtttttt gcgatcgcgg caaccggctt gttcctcttt
      181 gcccttggcc ggtacacggg attcttcacc ctggcttata atcattttcc aggcgtcgat
      241 cttttccgca ggcccgctga tgccacatac gcgcttggcg ggatggcctc gattttatcc
      301 ggctatcttt tgcatcgctt tttgtcggga agcgcgccaa caagccggca aaaacgaggc
      361 gttggcgtca tcctggccct tatcttcgca gtaagtttgg cggttgccgc tgctcattcg
      421 cacttctatc aggcggtcaa gccggtgctg ctttcgatcg gcatttttac cgccggctgg
      481 ctcagcctcg tttttgcaag gcaatactcc aatcgctatg cagtgctgac aatagcggtc
      541 ctggccgcct tcacgaccgc ggatcttgcg ataaacaatg gaccaaaccg gtcgacggcg
      601 cagcagcctt cctattacga ccaaatgcgc cctgatacca agaatgagac agtcgctttc
      661 ataaagacca atctcgtaca gccgctcaat tcgccacgac gagacagagt ggagatgata
      721 gggctcggct tcgagtggcc gaacatcagt ctcatccacg gtttcgacca tgcgcttgga
      781 tataacccgc ttcgcctggc ggaaattgcg gagggcggca tgggcgccag cgagacggtc
      841 gcagttgact atcagcgcga gttcacaccc ttgtatccat cataccgctc gatgatggcc
      901 gatctgcttg gccttcgata cattgtgacc gaccatccgg tcgagcagat cgacaagggg
      961 ctaaagcccg gagatctcac gcttgtcgcg aatacccgtg acgcctacat ctacgagaac
     1021 ccgcgcgcct tgccgcgtgt catgttcgcc ttcgattggc agcccgcaaa cttcgagcgg
     1081 attacgaaag acggacactg gccttatttc gatccgagac agatcgttct cttgcggggc
     1141 aggcagcaac tgatgagcac cctccaacca aggcccgttt ccctgtcgca agccgctacg
     1201 gctcttcagg tctatgggaa cacggaagtg caggtggtgg tcgacacacc gcgtgccggt
     1261 ttccttgtgc tgaacgacgt ttggcatcct tggtggttcg gcacagtgga cggcaagcct
     1321 gccgacattt tccgcgccaa tgttttattc cgggccatcg aagtgccggc gggcagacac
     1381 gtcgtccgtt tcgagttcaa gcccgtggaa ggagcggtga aggaaatcat ggcttacctg
     1441 acgggcaagc ctaacgagcc tacgccgccg ccctgggaac cttggctcaa gcctgaggcc
     1501 agcgctccgg cctttttgag aggcgccggg taagttcccg gaatttgctg cttgcgagcc
     1561 agtcgtattc aagggtgcgg gcccctctga tggagtcgct gccgtcgtgg tctccgcgct
     1621 ctgcgacgtg gcacatgacg agcggcgagg cgccttgaag gccttgaagc caatccgtcc
     1681 acagcgcttc taaatcggcg tcctcattga agtcatagac gccagcgaaa tcactgttca
     1741 tcccgtgccc gtgctccctc gcgaggtctt ccaatcggaa tgtcccggtt ctcgcgatga
     1801 tggcagcctt caggccgcgc catcgccgcg gagcgcaatt ccgaagactg atgcggcggg
     1861 catcatcgcc atatcgatcc gcgagcgcat cgataagagc ctccctgata aaagggagga
     1921 ggtggcaatg ctgatggcca tccacgaaat caggaagggc cttcatgctg gcctcgaact
     1981 gcgaaagctg acggtcgatc tccttccgca gaagcttccg gttcaaaacc cccgaatgcg
     2041 tgcgcatcgt gaggccggtg atcgtctgcc gcggaaagat gtccccggca aaagggctgg
     2101 taaaatcgag gtgcagcccg cgggaaagag gagcatccgc caaaggcccc gcggcctccg
     2161 gccaggtcgg agacagcacc atcgcgcttg tcgccgtcac cacgccctcg gcagcaagct
     2221 tcaggatcgc ggaatcgacg cacgcgtcca tcgcatagtc gtcggcgttg atgatcacac
     2281 cctcgtccat gatagcatcc cccgaagcta aggcatcgcg catgcaatcg gaccttgatg
     2341 agcgcgcgcc gggtgtcgtc cttgtatgca tctcgcaatt atcggcatcc acatgtcaat
     2401 ttcttcctgt cactcgcgtc cgtattcctt cttctgaaag atcatcagcg aaaacgtctt
     2461 cgctacaggt taacgagagc gtgagtccgt tccaaccccc gataatagtg agataattgc
     2521 gggaaaaata ccgaacggga cgctatcgcc gccgcctgcg cctcagctgt tccggcggtg
     2581 cgccagcgtt aattcgcgaa gccgtaacat aattaacgga cttttgctcg tcccagttaa
     2641 tgagaaaatt ccagcattta gttctattta atgggaggtc atgagcggat gaaaactttt
     2701 tggtttcaac gacatggcag aattacttcc caccgggacg aggcttcggc gggaagtaat
     2761 tgcggcagga ataaaatgct tgttaagtac aatggtgagc ggaaataaat ccggcgcgct
     2821 tgcgttaacc acatttgtat gaacactcgg tgcagcaacg aagcgcttac caagagagcg
     2881 c
//
LOCUS       DATMGX010000483         2951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_105948, whole genome shotgun sequence.
ACCESSION   DATMGX010000483 DATMGX010000000
VERSION     DATMGX010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105948"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VNR65_07810"
     CDS             complement(<1..319)
                     /locus_tag="VNR65_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ18615.1"
                     /translation="MCARVFDVIVVGGGHAGCEAAAAAARMGARTALVTHKFQTIGEM
                     SCNPAIGGLGKGHLVREVDALDGLMGRVIDRAGIQFRMLNRSKGPAVQGPRAQADRKL
                     YRLD"
     gene            635..1639
                     /locus_tag="VNR65_07815"
     CDS             635..1639
                     /locus_tag="VNR65_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWJ18616.1"
                     /translation="MQARDVMTKAVVTVTPEMPIGKIASLLLEKRISGVPVVDETGSV
                     VGIVTEGDLLKSNAPDREKRREWWLTLLAEGEDLSKDFLSTIRKNHRTAREIMSAPVV
                     TVSEDTDIAEIGKLFTTLQIKRVPVTREGRLTGVVARADLVRVVASQQSERHQEEGMF
                     AWTLRFDPFASQAKTPAKPVQDESPAEEDGLSATNFRHLVDDAASQKAHQVEHQRHAA
                     EEQRKARMKELIDHHLTDDHWRGVLHQARIAAEHGEKEILILQFPHELCSDGGRAINV
                     AEPDWPSTLRGEAAEAYLRFERDLKPRGFHLIAKVISFPEGYIGDIGLFLHWGGAKKA
                     "
     gene            complement(1654..2352)
                     /locus_tag="VNR65_07820"
     CDS             complement(1654..2352)
                     /locus_tag="VNR65_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HWJ18617.1"
                     /translation="MRCLGLLGGMSWESTAIYYRLLNEGVRSRLGGLHSAPLLLWSTD
                     FALIAERQAANEWEALSQQLSEAGRRLVCAGAEALIICTNTMHRLHADIARASGVPVI
                     HIADATAEALRKAGCKRPALLATRFTMEGDFYTGRLLDAFGIEAMIPDEAVRVQIHRI
                     IYDELCRGVVREESRILYRDAIDAMRRDGADSVILGCTEIAMLIGASDSELPLFDTTA
                     IHAEAGVAWIAGSG"
     gene            complement(2462..>2951)
                     /locus_tag="VNR65_07825"
     CDS             complement(2462..>2951)
                     /locus_tag="VNR65_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18618.1"
                     /translation="ASAEDADVALLMYDATTPYPMGEGPTAFLHEGLASKSMKTITVI
                     NKIDVAQPDLSRWPQETLLVSAKKGHGLDALRSALVRFAEESFGGSETPLITRVRHRQ
                     EIEHARSALNQFLGSAEASEHPEIAAEHLRASADALGRLTGRLDVEDVLGQIFSEFCI
                     GK"
BASE COUNT          635 a          897 c          859 g          560 t
ORIGIN      
        1 tatcaaggcg atagagcttg cggtctgcct gcgcgcgcgg cccctggaca gccggcccct
       61 tggaacggtt gagcatgcgg aactggatac ccgcccggtc gatcacgcgc cccatcagcc
      121 catcgagcgc atcgacctcg cggacgagat gccctttgcc aaggccgcca atcgcgggat
      181 tacaggacat ctcgccgatg gtttggaact tgtgtgtgac gagcgctgtt cgcgcgccca
      241 tacgggcagc cgccgcggcc gcctcgcaac cggcgtgtcc gcctcccaca acaatgacgt
      301 cgaatactcg tgcgcacatg ctcaaactct tcctgttgag caagacataa tcgactggcc
      361 gacgcaaagg aagcccgcgt tccgccgcct cctgtggagg atgccattgt cacaaacgca
      421 ggccatacag gagtcttggc gagcggcacc aggattgcta aatcgagccc agattgacag
      481 caagccagca caagcccagt ccgaggcgaa ggctttgttg agcgcaacgt aatgaatttc
      541 tcgtatacta atggctcagc acaagacggc ggtcgaatta gctcccttga gatgttccgc
      601 caatccttag gacccagaga agaaggaccc agctatgcaa gcgcgcgacg tcatgacgaa
      661 agccgtcgta acggtgacac ccgaaatgcc aataggcaag attgcttctc tccttctcga
      721 aaagcgcatc agcggagtgc cggtggtgga cgaaacaggt tccgtcgttg gcatcgtgac
      781 cgagggcgat ctcttgaagt cgaatgctcc cgaccgcgaa aagcggaggg agtggtggct
      841 caccctgctg gctgagggtg aagacctcag caaggatttc ctgtcgacga tccgcaaaaa
      901 ccatcggacc gcccgagaaa ttatgtccgc tcccgtcgta acggtaagtg aagacacgga
      961 cattgccgag atcggaaaac tcttcaccac actgcaaatc aaacgtgtcc ccgttacgcg
     1021 tgaaggccgg ttaacgggcg tcgtggcacg cgccgatctc gttcgcgtgg tcgccagcca
     1081 gcaatccgaa cgccaccaag aagaaggcat gtttgcctgg actctccgct tcgacccgtt
     1141 cgccagccag gccaagacgc ctgccaagcc tgtgcaggac gagtcgccgg ctgaggaaga
     1201 tgggcttagc gcaaccaatt tccgccatct cgtggacgat gcggccagcc aaaaagctca
     1261 tcaggtggag catcagcggc acgccgcgga ggagcagcgc aaggcaagga tgaaagagct
     1321 catcgaccac catctcaccg acgatcattg gcgcggtgtc cttcatcaag cgcggatcgc
     1381 ggcagaacat ggcgaaaagg aaatcctgat cctccagttc ccgcacgagc tttgcagcga
     1441 cggcggcaga gcgatcaatg tggctgagcc tgactggcca tcgaccttgc gcggcgaagc
     1501 agccgaagcc tatctgcgtt tcgagaggga tctgaagccg cgcggatttc acctcatcgc
     1561 caaggtgatt agttttcccg aaggctatat cggcgacatc ggcctgttct tgcactgggg
     1621 tggcgcgaaa aaagcctagc cgggaagccg tcctcaacca ctgccagcaa tccaggccac
     1681 gccggcttcg gcatggattg ctgtcgtatc gaagaggggc aactcactgt cgcttgcccc
     1741 tatgagcatg gcaatctcgg tacagccaag tatgacgcta tcggcgccgt cgcgccgcat
     1801 cgcgtcgatc gcatcccgat aaaggatgcg gctctcctcg cgcaccacgc cccgacaaag
     1861 ttcgtcgtag atgatccggt ggatttgcac ccgtaccgcc tcgtccggaa tcatggcctc
     1921 gatcccgaaa gcgtccagaa ggcgtcccgt gtagaaatcg ccttccatgg tgaagcgtgt
     1981 cgcgagaagt gcgggccgct tgcagcccgc tttccgcaag gcttcagcgg ttgcgtcggc
     2041 gatatggatg acgggcacac cggaagcgcg cgcgatatcc gcgtggagcc ggtgcatggt
     2101 attggtgcag atgatcaagg cttcggcgcc cgcgcagacc agtctgcgac cggcctcgct
     2161 caactgctga ctcagagcct cccattcgtt tgctgcctgt cgttcggcga taagcgcgaa
     2221 gtcggtcgac caaagcagca acggcgccga atgtaagccg cccaaacgag atctcacgcc
     2281 ttcattgaga agacgataat agatcgctgt gctttcccag ctcatgcctc caagcagccc
     2341 caaacagcgc attttgacct ccgctttcgt gtcggcgcga ttatcgctga caggccgtga
     2401 cggggaatcg ggattgcagc cagacccaac gtttcatgtg aaacaaagga ggctgtcgcc
     2461 cctacttccc aatgcagaac tccgagaaga tctggccaag cacatcctcg acatcgagcc
     2521 tgcctgtgag gcgtcctagc gcgtcggctg aagcccgtag atgctcggcg gcgatttccg
     2581 gatgttcgct tgcctcggcg ctgccaagaa actgattgag ggcgcttcgc gcgtgctcga
     2641 tttcctggcg gtgccggact cgagtgatga gcggggtctc gctgccgcca aagctctctt
     2701 ctgcaaaccg gacaagcgca gatctgagag catcaagtcc atggcctttc ttcgcggaga
     2761 ccaggagtgt ttcttgaggc cagcgtgaaa ggtctggttg cgcaacgtcg atcttgttaa
     2821 tcacggtgat cgttttcatg ctcttcgatg ccaatccctc atgaaggaac gccgttggtc
     2881 cctctcccat ggggtaaggg gtggtcgcgt cgtacatgag aagagccaca tcagcgtcct
     2941 ccgcgctggc c
//
LOCUS       DATMGX010000484         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_362964, whole genome shotgun sequence.
ACCESSION   DATMGX010000484 DATMGX010000000
VERSION     DATMGX010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362964"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VNR65_07830"
     CDS             <1..380
                     /locus_tag="VNR65_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HWJ18619.1"
                     /translation="HSIKIIEALFAAGKPVGAVCHGPAAFRHTKRPDGVPLVQGKSVT
                     GFSNSEEVAVGLTEAVPFLVEDMLKQNGGNYAKGPDWHPYVSIDENLVTGQNPKSSEG
                     VAWLFLDKLLNRETSSEQGPTAA"
     gene            complement(469..762)
                     /locus_tag="VNR65_07835"
     CDS             complement(469..762)
                     /locus_tag="VNR65_07835"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HWJ18620.1"
                     /translation="MSTKATWKPHTEHGKLTTKSDLPESVFAFPNQRKEPLTDASHVR
                     NALARFDQVEGVSGDDRDLAFANIKKAAEYYDVEVRERDWRELANSKHAKSRG"
     gene            complement(861..1565)
                     /locus_tag="VNR65_07840"
     CDS             complement(861..1565)
                     /locus_tag="VNR65_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWJ18621.1"
                     /translation="MQVTVRKSDDRGKANFGWLQSRHSFSFGHYYDPGHMGFGPLRVI
                     NEDRVAPGGGFPTHPHRDMEIISYVLDGALEHKDSLGTGSVIRPGDVQRMSAGSGVRH
                     SEYNASSKEPVHFLQIWIIPEREGLKPGYEQKGFEAAEKRGKLRLIGSRSGRDGSVTI
                     HQDADLYATLLERGESVTHALKAGRSAWVQVASGSIAVNGQKLEAGDGMAVEGAGDLS
                     LEAEASAEVLLFDMAA"
     gene            1745..>2249
                     /locus_tag="VNR65_07845"
     CDS             1745..>2249
                     /locus_tag="VNR65_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ18622.1"
                     /translation="MDKLDAMNAFVKVVALGSYAEAGRALGLTRSAVSKAVMELEQLL
                     GARLLDRTTRRVGPTEAGLAYYERCLDIIGRVEETEMEVSRLHTEPKGVLRVNAPMSF
                     GALYLGPAAADFMQDYPDLKIELTLNDRFIDPIEEGVDVTIRIASLADSSLIARKLAP
                     ARRVLAAA"
BASE COUNT          411 a          692 c          676 g          470 t
ORIGIN      
        1 cacactcgat caagatcatc gaagccctct tcgcggcggg caagccggtg ggtgctgtct
       61 gccacggacc ggcggcattc cgccatacga agcgcccgga tggcgttcct ctcgttcaag
      121 gaaaatcggt caccggcttc tcgaactcgg aagaggtcgc agtcgggcta accgaggccg
      181 tccctttcct ggtagaggat atgctgaagc agaacggcgg caactacgcc aagggtcccg
      241 attggcaccc ttacgtgagc atagacgaaa atctcgtgac cgggcagaac ccgaaatctt
      301 cggagggcgt tgcatggttg ttcctggaca agctcttgaa ccgggaaaca tcgagcgaac
      361 aaggccctac ggccgcgtag ggtcaggcat ttccgaagga gcgaggtctt cagcgctgct
      421 caaggcccag ctcttggttt cctcgcggaa cgcctgtcgt tccactcttc agccgcggga
      481 tttggcgtgc ttgctattcg ccaattcccg ccaatctcgc tcgcgaacct cgacgtcata
      541 atactccgcc gccttcttga tgttcgcgaa tgccaaatcc cgatcgtcgc cggaaacgcc
      601 ttcgacctga tcgaagcgcg cgagcgcgtt gcgcacgtga ctggcgtccg tcagtggttc
      661 tttgcgctgg ttgggaaagg cgaagacact ttcgggcaga tcgctcttgg tcgtaagttt
      721 tccgtgttcg gtgtgaggct tccatgtggc cttggtggac atcgggtgtg ctcctttagg
      781 gaaggacaac accgatcagc cgggaaggtc acccggcatc acccggaccc atcggagcgt
      841 cagcgtggaa ggcctccgcg ttaagccgcc atatcgaaaa ggagcacttc cgcgcttgct
      901 tccgcctcca aggagaggtc gcctgcgccc tcgacagcca taccgtcgcc tgcttccagc
      961 ttctggccgt tgacggctat agagccactg gccacttgca cccaggcgct ccgtcccgcc
     1021 ttaagggcat gcgtaacgct ttcgcctcgc tccagcagcg ttgcatacag atcggcgtcc
     1081 tggtggatgg tgacggaacc atcgcgaccg ctgcgtgagc cgatgaggcg aagcttgccc
     1141 cgcttttcgg cggcctcgaa gcccttttgc tcatagccgg gtttgaggcc ctcacgctct
     1201 ggaataatcc agatttgcag gaagtggaca ggctccttgc tggaggcatt gtattcgctg
     1261 tgacggactc ccgacccagc cgacatccgc tgcacgtcgc cgggccggat cactgagccg
     1321 gtcccgaggc tgtccttatg ctccagggca ccatcgagga cataggagat gatctccatg
     1381 tcgcggtgcg gatgcgtggg gaagcctccg cccggcgcga cacggtcctc attgatcacg
     1441 cgaagcggcc cgaagcccat gtgaccgggg tcgtagtaat ggccgaacga aaagctgtgc
     1501 ctgctttgaa gccagccgaa attcgctttg ccgcggtcgt cggattttct caccgttact
     1561 tgcatcgtcg ttttccttct ttggacgctc gctcttgttt ctgggttcgc ttgtcgcgtg
     1621 tctggatgga gagcgatctt tctgcgtccg tgttccggtt atgaggcgaa tgtattccgt
     1681 ctctggcgac gtacaatctc ataagatttc accagaccgc ttccaaaaca gatacaatcc
     1741 tcccatggac aagcttgatg ccatgaacgc cttcgtgaaa gtggttgcgc tcggcagcta
     1801 cgctgaagcc ggtcgcgcac tcgggctgac gcggtcggcc gtcagcaagg ccgtgatgga
     1861 gctcgaacag ttgctcggcg cgcggctcct cgatcgaacc acaaggcgcg tcggtccaac
     1921 cgaggcgggc ctcgcctatt atgagcgctg cctcgacatc atcggccgag tcgaggaaac
     1981 cgagatggag gtgtcgcggc tgcataccga gccgaagggc gtgctcaggg tgaacgcgcc
     2041 catgtcgttc ggggcgctgt atctcgggcc ggcggccgcg gactttatgc aagactatcc
     2101 cgacctcaaa atcgagctga cgctcaacga ccggtttatc gatcccatcg aagaaggggt
     2161 ggacgtcacc atccggatcg cgagccttgc cgattccagc cttatcgcgc gcaagctggc
     2221 gcccgcccgc cgggtgctcg cggcggcgc
//
LOCUS       DATMGX010000485         2923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_416488, whole genome shotgun sequence.
ACCESSION   DATMGX010000485 DATMGX010000000
VERSION     DATMGX010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416488"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..607
                     /locus_tag="VNR65_07850"
     CDS             308..607
                     /locus_tag="VNR65_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18623.1"
                     /translation="MNADTLETVCGSNGSEADHGSCEDIETRLRDMREIVDEIERNYS
                     LDEISADDWKIYVEYCAALGQPVSGRDLLGKKHPELYPEEIDFMLSPRNDGECPF"
     gene            775..>2923
                     /locus_tag="VNR65_07855"
     CDS             775..>2923
                     /locus_tag="VNR65_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18624.1"
                     /translation="MLPSVTQTPDVLQVTFVEIDEEGKVTKRFPRIDAEGQLKNTVLH
                     KRYPEILVAVVSVPKLQLDAYEEAKVREALMRQRIDGVEYRLVGASGSAKDGKYYAVD
                     AEHEKPIAERFQKWPQAAITYFGILVSDCRVVIEAQDERVLVVEDHALGTNDCRGWIS
                     ERLFRKLCLPDRRFYQFRLAFDRTQAKGSFKVMQDDVAEILDADIILPESSVKPRLEL
                     GLLGECQRLFAGLSSGIQGTRFRSPIVLGIRDVSRDLQFKSSYTLIEHAPPDSLQTEI
                     IPRALEEVRSVVGSALAGDYRKLLETIGTSAVQGQIQDAEDDEYTSYESSVVEAVLKA
                     DGSGSLVRHPYLNSRLQQLLVKWAFKASTGGGFRLPAFALADDGYLFAHDGQVFAGSD
                     WIPEGCGVGSLSSRRGLVIRYPIRMKEDLLPVELLSVEATVQTLQRALGSTGCRMEPP
                     QIADLVDSQLRLQGTFVLHSKTAARNGGDFDFDLVAILPASEFPRFVQSRFEMREEHR
                     RHKDKQKKVKSPWWNLADVAMKARGNQIGRITNLISDSIAYGRPDCAYELVDQLHNAL
                     DSLKHNVAVDQAVIKRIRGEVPKAPWRDAKEARRISQMPMDVPVCGTDIVGQLYNYVR
                     KEIGILFEETLPLRDFGGMIRGETFTREMHEECVKVNRAFGIKATELRKGTEQFEAAL
                     REAQAEFERLRKDDNPQVRQQASKSLYRAKAAIR"
BASE COUNT          643 a          810 c          921 g          549 t
ORIGIN      
        1 aagcatgatc gccggcgtga ttctgctgtg gacgttgcag aaccagccgt cggttggcag
       61 gtacatccgt gagttgctct cgtttgcgcg gggcggaccg cggattaaac agtatcgcgg
      121 tgattcaacc tgcaaggatt gagttctcgc ggtaagtgaa gcggatcggg tccgccatag
      181 ggcgtgccgg ttcttaacaa ctcaggcaat gtcgctggcc ggctcttcgg gcgcagggtg
      241 gaagaagagg ccgcgagcgg ctccttttct ccaccaggct gttcgcggac cggggaaagg
      301 agcagcaatg aacgccgaca cgcttgaaac agtgtgcggg tcgaatggaa gtgaagccga
      361 tcacggttct tgcgaagata tcgaaacgcg cttgcgggac atgcgcgaga tcgtggatga
      421 gatcgagcgg aactacagtc tggacgagat ttcggcagac gactggaaga tctacgtgga
      481 gtactgcgcg gccctcggcc aaccagtctc cgggcgggat cttctaggga agaagcatcc
      541 ggaactctat ccagaggaaa tcgacttcat gctctcgccg cggaacgatg gcgagtgccc
      601 gttctagacc attaacaact gaataacagc gcaggatggc ggtccggctc ttccgagcca
      661 ggccgaaaac gtaggcaagt tttcggcaca ggagcagagg aagaaaccgg cgccggttcc
      721 ttctctctgc tcgtgcgccg caatatcaac cacgacacag agaaaggaaa gaccatgttg
      781 ccatctgtta cacaaacgcc ggacgtgctt caagtgacgt tcgtggaaat cgacgaggaa
      841 ggtaaagtga cgaagcgatt ccccaggatc gacgccgaag gccaactgaa aaacacggtg
      901 ctccacaagc gttatccgga gattctcgtc gccgttgtca gcgtacccaa gctccaactg
      961 gacgcgtacg aggaagccaa ggtccgggaa gcgctcatgc gccagcggat cgacggggtc
     1021 gagtaccgtc tggttggcgc aagcggctcg gcgaaagacg gcaagtatta cgccgttgac
     1081 gctgagcacg aaaagcccat cgccgagcgg ttccagaagt ggccgcaggc ggcgatcacc
     1141 tacttcggca ttctggtctc ggactgccgg gtggtgatcg aggcgcagga tgagcgcgtc
     1201 ctcgtggtgg aagatcacgc tctgggaacc aacgactgcc gcggatggat ctcggagcgg
     1261 ttgttccgca agctgtgctt gccggatcgc cgcttctatc agttccggct ggcgttcgac
     1321 cggacccagg cgaaaggctc attcaaagtg atgcaagatg acgtggcaga gattctcgac
     1381 gccgacatca tcttgccgga aagctcggtt aagccgcggc ttgaactggg gctgctcggg
     1441 gagtgccaga ggttgtttgc agggctttcg agcggcattc agggaacgcg tttccggtcc
     1501 ccgatcgtgc tgggaatccg ggatgtatcg cgcgacctgc agtttaagtc gagctacacc
     1561 ctgattgaac acgcgccgcc cgactctctt cagaccgaga tcattcctcg ggcgctggag
     1621 gaagtccgca gcgttgtggg ttctgccctc gccggcgatt accggaaact tctcgaaact
     1681 atcgggacgt ccgccgtaca agggcaaatc caggacgccg aagacgacga atacacatcc
     1741 tacgagtcgt cggtcgtcga ggcggttctg aaagcagatg gcagcggctc gctggtccgg
     1801 catccgtacc tgaactccag actgcaacag ttgctggtga agtgggcgtt caaggcgtcc
     1861 actggtggcg gattccgtct gccggcgttt gccttggcgg acgacggata cttgttcgcg
     1921 catgacggac aggtgttcgc cggttccgat tggattccgg aggggtgcgg tgtcggctcg
     1981 ctctcatcaa gaagagggct ggtgatccgc tatccaattc ggatgaaaga ggacctgctt
     2041 ccggtggaac tgttatcggt tgaagccacg gtccaaacac tccaacgggc gcttgggtcc
     2101 accggttgcc gcatggagcc gccacagatc gcggatctgg tcgatagcca gctcaggctc
     2161 caagggacct tcgtgttgca ctcgaagact gcggcgcgga atggcggcga ctttgacttc
     2221 gacctcgtgg ccatcctccc ggcaagcgaa tttccccggt tcgtccagag ccggttcgaa
     2281 atgcgcgaag agcaccgccg tcacaaggac aaacagaaga aggtcaaatc tccgtggtgg
     2341 aatctcgccg atgttgccat gaaggctcgt ggaaatcaga tcggccgaat caccaaccta
     2401 atcagcgatt cgatcgcata tgggcggccc gactgcgctt acgaattggt ggaccagctc
     2461 cataacgcgt tggattcgct caaacacaac gttgcggtgg accaggctgt gatcaaacgg
     2521 atccgcggcg aggtccccaa ggcaccctgg cgtgacgcaa aagaagcccg gcgcatctca
     2581 cagatgccca tggacgtgcc cgtctgcggc acggacatcg tcgggcagct ttacaactac
     2641 gtccgcaagg aaatcggcat tctcttcgag gagacacttc cgctgcgcga tttcggcggc
     2701 atgattcgcg gagagacttt cacacgagag atgcacgaag agtgcgttaa ggtgaaccgc
     2761 gccttcggca tcaaagcaac cgaactgcgg aaggggacgg agcagttcga ggcagccctc
     2821 agggaagcgc aagcggaatt tgagcggctg cgcaaagacg acaatccgca agtccgtcag
     2881 caggcctcga aaagtctcta ccgggccaag gccgcgattc gtg
//
LOCUS       DATMGX010000486         4418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_201727, whole genome shotgun sequence.
ACCESSION   DATMGX010000486 DATMGX010000000
VERSION     DATMGX010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201727"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="VNR65_07860"
     CDS             complement(<1..1411)
                     /locus_tag="VNR65_07860"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HWJ18625.1"
                     /translation="MKNLLGGKGANLAEMSNLGLPVPPGFTITTDVCNAYYALGRKLP
                     EGLKEQVEESVIYIGKTVGANLGDAQNPLLVSVRSGSRASMPGMMDTILNLGLNDETV
                     KGLAARSGDKRFAYDSYRRFIQMYSGVVLDVKYHNFEELLEHYKHQNGYDLDTDLTAE
                     DWQSLIVQYKDVVQRKLGRPFPQDINEQLWGAIGAVFDSWNNARAIKYRQLNGLPESW
                     GTAVNVQAMVFGNRGDTSATGVAFTRNPSTGDPRLYGEFLVNAQGEDVVAGIRTPQPI
                     TEVARKESGGDKPSLEAIMPAAFKEFAAICQKLERHYRDMQDIEFTIQEGTLWMLQCR
                     NGKRTTPAALKIAVDFAREGLINKEEAITRIEASQLDQLLHPTIDPSAKRNIIATGLP
                     ASPGAACGEIVFSPDDAEKLKGQGKTVILVRTETSPEDIHGMHAAEGILTARGGMTSH
                     AAVVARGMGKPCVSGAGAMR"
     gene            complement(1884..3416)
                     /locus_tag="VNR65_07865"
     CDS             complement(1884..3416)
                     /locus_tag="VNR65_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HWJ18626.1"
                     /translation="MFAQVSTVAFEGIDAKQVDVQVLIAPGLPAFSIVGLPDKSVGES
                     RERVRAALTSIGLGLPAKRITVNLAPADMPKEGTHYDLPIALGVMAAAGAISPDVLKD
                     FIIIGELGLNGGLGAIAGALPAALGANGLEKGLICPAACGPEAAWASSSMPIVAPPHL
                     VALVNHLKGTALIPQPQPHLDQSTEAVPDLKDVRGQESAKRALEVAAAGGHHMLMIGP
                     PGAGKSMLAQRLRSILPPLEPQELLEVAMIQSIAGVITNGTLTSNRPFRAPHHSASMA
                     ALVGGGARPRPGEVALAHHGVLFLDEFAEFPPHVLDALRQPIESGETIIARVNHRIRY
                     PSRFQLVAAMNPCRCGHAWTPGVTCRHGTRCEESYRARISGPLWDRLDIQIAVAPVKA
                     TDLALPPSRETSADVAERVATARSIQARRYSMIGNDVVTTNARCPAALLEEFCGLDRE
                     GMRLLELAAEANGLSARGYHRALRLARTLADLDASADIHPVHVAEALSLRGDGFGLRR
                     AA"
     gene            complement(3489..>4418)
                     /gene="gshB"
                     /locus_tag="VNR65_07870"
     CDS             complement(3489..>4418)
                     /gene="gshB"
                     /locus_tag="VNR65_07870"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF003573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HWJ18627.1"
                     /translation="DPIEKIHISGDSTFALLLEAQHREHSIFYYTPDKLFMRDGRVLA
                     EGHELRVDDVHGAHYELGAKQTLELRELDVIHLRQDPPFDMAYVTTTHILDHIHPKTL
                     VVNDPASIRNAPEKLFVTEFPELMPPTLITRSLEAVNAFRAEFGDIIVKPLYGNGGAA
                     VFRVRPDDTNTGSLVELFQTVFREPFVVQQYLPDVRKGDKRIILVDGKPVGAINRVPA
                     ENETRSNMHVGGRPEAAELTERELEICEAIGPELQRRGLIFVGIDVIGDYLTEINVTS
                     PTGIREVKRFGGPDIAALIWDAVEEKICSSKVR"
BASE COUNT          879 a         1331 c         1271 g          937 t
ORIGIN      
        1 cgcgcatcgc gccggcgccg gagacgcatg gcttgcccat gccgcgggcc accacggccg
       61 cgtggctggt catgccgccg cgggcggtga ggatgccctc cgccgcatgc atgccgtgga
      121 tgtcctcggg gcttgtttcg gtccgcacaa ggataacggt cttgccctga cccttcagct
      181 tctcggcatc gtcaggagag aagacaatct cgccgcaggc ggcgccgggg gacgccggca
      241 ggccggtggc aatgatgtta cgcttcgccg agggatcgat ggtcggatgg agaagctgat
      301 cgagctgaga cgcctcgatg cgggttatgg cttcttcctt gttgatgagc ccttccctgg
      361 caaaatcgac cgcgattttc agcgccgccg gcgtcgtacg cttgccattg cggcactgca
      421 gcatccagag tgtgccttcc tggatggtga actcgatgtc ctgcatatcg cggtagtgac
      481 gctcgagttt ttggcagatc gctgcaaact ctttgaacgc ggccggcatg atcgcctcaa
      541 gcgaaggctt atctccgcca ctctcctttc gcgccacttc cgtaatcggc tgcggcgtgc
      601 ggatgccagc cactacgtcc tcgccttgtg cgttgacaag gaactcgcca tacagccttg
      661 gatcgccggt cgacggattg cgcgtaaagg cgacgcccgt tgcgctcgtg tccccgcggt
      721 tgccgaagac catcgcttgg acgttcacgg ctgtgcccca gctttcaggg agaccgttca
      781 gttgccggta tttgatggcg cgtgcattgt tccagctgtc gaacaccgcg ccgattgcgc
      841 cccaaagctg ctcgttaatg tcttgcggaa acggccgtcc gagtttgcgc tgcacgacgt
      901 ccttgtattg gacgataagg gattgccagt cttcggccgt gaggtcggta tcgagatcgt
      961 agccgttctg gtgcttatag tgctccagaa gctcctcgaa gttgtgatat ttcacatcga
     1021 gcaccacgcc cgagtacatc tggatgaagc ggcgatagct gtcataggcg aagcgcttgt
     1081 cgcctgagcg cgcagcgagc ccttttaccg tttcatcgtt caggcccaag ttcagaatcg
     1141 tatccatcat gcctggcatg gacgcccggc ttccggaacg caccgaaacg agaaggggat
     1201 tttgggcgtc tcccagattg gcgcctaccg tcttgccgat atagatgaca gactcttcga
     1261 cttgctcctt gagcccctcg ggcaatttcc ggccaagcgc gtaataagcg ttgcagacgt
     1321 cggttgtaat ggtgaagcca ggaggcacgg gcaaaccaag gttcgacatc tcggcgagat
     1381 tggcaccctt gccgcccagc agatttttca tttctgccga gccatcagct tttccagcgc
     1441 caaagcgata aacaaacttt tcagtccctg gcattttcac tcctccgaga gatcgcggcc
     1501 ctgtcggtgc cgcgggtttc ttcgcgcgac ctttcccgtc cttactggtc aaatcaacgg
     1561 ctgtcccccc tcatcaagga ggcaaagctt ttagcaaggc gcaggtttgt ttctaaaatg
     1621 ttgatagtga tgtcatggat gaggcgcgct ggcgtaaaac aggacattca tcagcgcgca
     1681 ttttcccctg gcgtcacttt gcggctcttc tttctcaaaa aggtagccgg cgatacacct
     1741 ccggcgccta cgccacctcc ggcccattca tagtatacat taagtcttat gtaactgaga
     1801 tgcgcggcgc gctcaagcgt tagagcagcc ctgcgccatc gaggaccatg ttaacgcaaa
     1861 aatctatttc acccaaggaa accttaagcg gcacggcgta acccgaatcc gtcgccgcga
     1921 aggcttaacg cctcggcgac atgaacagga tggatatcgg cgctagcatc aaggtctgcg
     1981 agtgtcctgg caaggcggag cgccctgtga tagcctcggg ctgataagcc attcgcttct
     2041 gcagccagtt ccaataaacg cataccctcc cgatcgagcc cgcaaaactc ttctagcaat
     2101 gcagccgggc atctcgcgtt tgtcgttacc acatcgttgc caatcatgga atagcgccgg
     2161 gcctgaatcg accgagccgt ggcgaccctt tcggccacat ccgcgcttgt ctcccgcgaa
     2221 ggcgggagcg caagatcggt cgctttcacg ggcgccaccg caatctgaat atcgaggcga
     2281 tcccaaagcg gtcccgaaat tcttgccctg tagctttcct cgcacctcgt tccatgcctg
     2341 caggtcacgc ccggcgtcca ggcgtggccg cagcggcatg gattcatggc tgccacgagt
     2401 tggaatcgcg acggatagcg tatgcggtgg ttgacgcgcg caatgatcgt ctctccgctc
     2461 tcgattggct ggcgaagcgc gtcgagcaca tgcggtggga attccgcgaa ctcatcaaga
     2521 aagaggacgc cgtgatgggc gagcgcgacc tctcccggtc tgggccgggc cccgccgcct
     2581 acgagcgccg ccatgctcgc ggaatgatga ggcgcgcgga aaggccgatt cgaagtgagc
     2641 gtgccgtttg ttatcacgcc tgcaatgctt tggatcatcg caacctcaag aagctcctga
     2701 ggctcaagcg gcggaaggat gcttcggagc cgctgagcca gcatcgactt gcccgctccc
     2761 ggtggcccga tcatgagcat gtgatggccg cccgccgccg cgacctcgag cgcgcgcttg
     2821 gcgctctcct ggccgcgcac atccttgaga tcaggaaccg cttcggtgct ttgatcgaga
     2881 tgcggctgag gctgcggaat aagtgccgtt cctttgaggt gattgacgag agccaccaga
     2941 tgcggaggag ccacgatcgg cattgaggag gaggcccaag ctgcttccgg tccgcaggcc
     3001 gcgggacaga tcagcccctt ttcgaggccg tttgcgccga gcgcggccgg caaagcgcct
     3061 gcgattgcgc caaggccccc gttcaggcca agttcaccaa tgatgatgaa atccttcaac
     3121 acgtccgggg agattgcccc agccgccgcc atgacgccca acgcgatcgg cagatcgtaa
     3181 tgcgtccctt ccttcggcat gtctgctgga gcaaggttga cggtgatccg tttcgcgggc
     3241 agaccaagac caatagacgt taacgccgcg cgcactcgct ctcggctctc tccgaccgat
     3301 ttgtccggga ggccaacaat cgaaaacgcc ggaaggccag gcgcgataag aacctgcacg
     3361 tcgacttgtt tcgcgtcgat gccctcgaaa gcgaccgtgg acacctgcgc aaacattcca
     3421 acacctccac caacaccttc aatgaataca gcagatatta tattcatttt ttataaaatc
     3481 aagaacaatc agcgaacctt agaagaacaa atcttctctt ccacggcgtc ccagataagg
     3541 gcggcgatat cgggtccgcc aaagcgtttg acctcacgaa tgccggttgg cgatgtaaca
     3601 ttgatttcgg tcagatagtc tccgatgacg tcgatgccga cgaagatgag gccacgccgc
     3661 tgaagctcag gcccgatcgc ttcacagatt tcgagttccc gctcggtgag ctcggcagcc
     3721 tccggccggc cgcccacatg catattcgac cgcgtctcat tctccgcggg cacgcgatta
     3781 atcgcgccaa ccggcttacc atccacgagg atgatgcgct tatcgccctt tcggacatcg
     3841 ggcagatatt gctgaacgac gaaaggttca cggaacacag tctggaagag ttccacaagc
     3901 gagccagtat tcgtgtcgtc gggccgcact ctgaagactg ctgcgccgcc gtttccgtat
     3961 agcggcttga caatgatgtc tccaaactcc gcacggaagg cgttcacagc ctcgagggag
     4021 cgcgtgatga gtgtcggcgg cataagctcc gggaactcgg tcacaaacag cttttcgggg
     4081 gcgtttcgaa tgctggccgg gtcgttcacg accagcgttt tgggatggat gtgatcgaga
     4141 atgtgcgttg tggtcacata agccatgtcg aacggcgggt cctggcggag gtggattaca
     4201 tccagttccc tgagttccag cgtttgcttc gcccccagtt catagtgagc gccatgcaca
     4261 tcgtcgacgc gaagctcatg gccttcggca aggacccgtc cgtccctcat gaatagcttg
     4321 tcgggcgtgt aataaaaaat gctgtgctcg cgatgctgcg cctcaagcag gagcgcaaat
     4381 gtcgaatcac cgctgatatg aattttctcg atcgggtc
//
LOCUS       DATMGX010000487         2110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_430403, whole genome shotgun sequence.
ACCESSION   DATMGX010000487 DATMGX010000000
VERSION     DATMGX010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430403"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /gene="tnpB"
                     /locus_tag="VNR65_07875"
     CDS             complement(<1..306)
                     /gene="tnpB"
                     /locus_tag="VNR65_07875"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HWJ18628.1"
                     /translation="MLKVAPGSRIFMAVKPVDMRRSFDGLCATVTEALGGDPLSGDVF
                     LFRGKRADRIKAIAWDRTGLAIWYKRLERGKYKWPARDAASIEITEHEVALLLDGVDF
                     "
     gene            complement(300..695)
                     /locus_tag="VNR65_07880"
     CDS             complement(300..695)
                     /locus_tag="VNR65_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18629.1"
                     /translation="MSATQLSPSRRREDIRAWWAAHMKAQQDSGQSQVAYCRARGLDP
                     KYFTLWKRKLREPRIPAKTVEPAQLVPVVVRTNRTVSASPMRPEKRLSEPTGIVALRL
                     SLGNGISLSLKVATGWIPMLVRELAGLRC"
     gene            complement(1142..1342)
                     /locus_tag="VNR65_07885"
     CDS             complement(1142..1342)
                     /locus_tag="VNR65_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18630.1"
                     /translation="MPLRKGSSQSAVSSNIKTLVDDWKKDGSIGTSHPPTKKKAIKQA
                     VAIALGKAGKSRNTLSRRQKAI"
     gene            complement(1577..2056)
                     /locus_tag="VNR65_07890"
     CDS             complement(1577..2056)
                     /locus_tag="VNR65_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWJ18631.1"
                     /translation="MGSTADRIKDIGPQPQSFDIESATKENTNYRSVAWSGRYLQVTL
                     MLIPAGGDIGLEAHPETDQFLRLDAGSGRVQMGAAKDKLTFEKEVSNGWCVLVPAGTW
                     HNITNIGATPMQVYAIYAPAHHTPGKVQATAAAAAADKDDEPAAWSVQPKHAPDKHG"
BASE COUNT          406 a          665 c          572 g          467 t
ORIGIN      
        1 gaagtccacg ccatcgagca ggagggccac ctcgtgttcg gttatctcga tggatgcagc
       61 gtcccgcgcg ggccacttgt acttgcccct ctcgagtctc ttgtaccaaa tcgcgagtcc
      121 cgtccggtcc caggcgatgg ctttgatccg atccgcccgc ttgccgcgaa acaagaacac
      181 atcgcccgaa agcggatcac cgccgagggc ctcggtgacc gtggcgcaca ggccgtcgaa
      241 ggaacggcgc atgtccactg gcttcacggc cataaagatg cgcgaaccgg gcgccacttt
      301 cagcatcgga gcccagccag ctcgcgcacc agcatcggaa tccacccggt ggccaccttg
      361 agcgacaaac tgatcccatt gccgagactt aagcgaagag ccacaatccc ggtcggctcg
      421 ctcaaccgct tctccgggcg catcggcgat gcggatacgg tcctattggt ccgcaccacg
      481 accggaacca gttgagctgg ctcgactgtc tttgcaggaa tccgtggctc gcgtagcttg
      541 cgcttccaga gggtaaagta ctttggatcc aaaccccgcg cacggcaata agccacctgg
      601 ctttgccctg aatcctgctg cgccttcata tgcgccgccc accaggcccg aatatcttcc
      661 cgccgccgcg atggcgataa ttgtgtcgcg ctcatccgtc aactccaggt cctaggcctg
      721 cggcgaactc tggcgaagcc ggccggctgc ggaaatacgc ggattagcgg acgcatacga
      781 attagcaatg gccggggcta cgtatcaata gagcaagccg aatccatcgc ggaagaactg
      841 agtcagtgca tcaaggcgaa ccgagctatt cccccgcacc tccgccctac gacccaatgc
      901 tcctcaatga actcgcgaaa gtgttcgccg agggcaccct ggcgactcgc cggatagagg
      961 aatctctgag ttcttcagcc actttggcga ccgtctacgc cgcaaccggc accgcggctg
     1021 ggggacacgt cagcgcaaag tcgatactct caagatctcg tatattcagt acttaattcc
     1081 gtggccacta acttttcgca atgacacggt gcaggcatgc ctggatggaa tgaaacccga
     1141 ctcagatggc cttttgcctc cgcgacagcg tattacggct tttgccggct ttgcccaatg
     1201 cgatggcaac ggcctgcttg atcgcctttt tctttgtcgg cggatgactg gtgcctatcg
     1261 acccatcctt cttccagtca tccaccagtg ttttgatatt gctgcttacg gccgactggc
     1321 tggatccttt tctgagaggc ataatctttc tcctgtctcc ggaatcaact gaacatggct
     1381 ttcacaacgc gcggcgcggc aaccggatgt tcctctccgc ctgcgcaatg ctctattcac
     1441 cagtggatag aatcgaacac ttggtgcccg ttggccactc ttgcatcgag atggattgcg
     1501 gacatccgat ccggcagttt catagtccgc ttggcgccat tagctgacat gcttacgttc
     1561 ttgcagatca atcgggtcac ccgtgtttat cgggcgcatg ttttggctgc accgaccaag
     1621 ccgctggctc gtcgtctttg tcggctgcgg ccgctgccgc cgtcgcctgc accttgccgg
     1681 gcgtgtggtg ggcgggcgcg tagatcgcat agacctgcat tggagtcgcc ccgatattgg
     1741 tgatgttgtg ccaagtcccc gccggaacga ggacgcacca accgtttgaa acttcctttt
     1801 caaaagtcag tttgtccttc gcggctccca tctgcactcg gccgctacca gcgtcgaggc
     1861 gcaggaactg atcggtctcc ggatgagctt caaggccaat gtcgccgccg gcggggatca
     1921 acatcaacgt cacttgcaga tagcgcccgc tccaggcgac tgaacggtaa tttgtatttt
     1981 ccttcgtcgc gctttcaatg tcgaacgatt gcggctgtgg cccaatgtct ttgatcctgt
     2041 cggcagtgct gcccatgact tgtctcctga aagagggaag ggctccgttc cctcgattgt
     2101 ctctgaaact
//
LOCUS       DATMGX010000488         3645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_265046, whole genome shotgun sequence.
ACCESSION   DATMGX010000488 DATMGX010000000
VERSION     DATMGX010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265046"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..990)
                     /locus_tag="VNR65_07895"
     CDS             complement(7..990)
                     /locus_tag="VNR65_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18632.1"
                     /translation="MSNLYTASEANDLINAGKRLHIAGDESALTQLNKGVWIGGTIPY
                     FLTQQGGVMDRQRLFVTELPDCVSETKISFVSSDQLASVPEEAPDNGFSLVIVPGMSE
                     VHAAYALKAEHLPHIYERPVIGWVAGVHLSEIGTVKPKVVNGMTGEISDDRLVVLHAA
                     LPDNRLASLGIVNVFRQGRGDTFVFDKTRFTGGDCLINGKSGNFYDYAKDRNIDLRFP
                     LVANYSGEMINVSIQELDQATRSVKFYAPVLEGMEYRQAEPVADYRAKFTEQMEDLKI
                     SPAFSCNCILNYLYGNLEGQQPIPIGGPATFGEIAFVLLNQTMVFMIINEV"
     gene            2260..3453
                     /locus_tag="VNR65_07900"
     CDS             2260..3453
                     /locus_tag="VNR65_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWJ18633.1"
                     /translation="MSESNQADVIIVGAGPAGLGAALGCLKAGLAPAVVDTSKSASTY
                     ASKGRSAALFNKTIAFLQRLGVWDGCKAHAEPLKALQFIDDTGRRLRAPDCVFHAEEI
                     GEAAFGYNITNADLVAAFQAEVEKQNLPIITPGPLTSFRETATKASLRFADGTELRAP
                     LAIAADGRMSATREAVGIRKLSWAYDQIAMAASFSHERPHRGVCIEFHRSAGPFTLVP
                     LPGNASSFVWVERKNDAERLLALDDADFAFEIEKASHLALGRVSSVSDRARFPLSSLA
                     VREYGRTRVALVGEAAHVTPPIGAQGLNLGFRDVETLIGLLAAAKEKKEDLGSDDLLQ
                     SYSAARRGDIMSRTVGADILNRSLLSDILPLQAARGVGLYALAAIGPLRRAFMRRGMA
                     PVGFV"
BASE COUNT          778 a          998 c         1031 g          838 t
ORIGIN      
        1 tccgatctaa acttcgttga tgatcatgaa gaccatagtt tgattgagga ggacgaaggc
       61 aatttcgcca aaagtggccg gcccgccgat aggtatcggc tgttgaccct caagatttcc
      121 atagaggtaa ttgagaatgc agttgcaaga aaacgcaggc gagatcttca agtcttccat
      181 ttgttcggtg aatttcgccc tgtagtcggc gacgggttcg gcttgccggt attccattcc
      241 ttccaggacc ggtgcataga atttcaccga ccgcgtcgcc tgatcaagct cctgaatgct
      301 gacattgatc atctcgcccg agtaattcgc gaccagcgga aagcgcaggt cgatgttacg
      361 gtctttcgcg taatcgtaga aattgccgct cttgccgttg ataaggcaat cgcctcccgt
      421 gaagcgcgtc ttatcaaaga cgaaggtgtc gcctcttcct tgccggaaga cgttgacgat
      481 accgagactg gccaatcggt tgtcgggaag cgccgcgtgc agaacaacga ggcggtcatc
      541 cgaaatttcg ccggtcatcc cgttgacgac tttcggtttg accgtgccta tctcgctgag
      601 atgaacaccc gcaacccacc cgatgaccgg cctctcatag atgtggggca ggtgttcggc
      661 cttgagcgcg tacgcggcgt gaacctccga catccccggc acgatcacga gcgaaaagcc
      721 gttatctggc gcctcttccg ggacggacgc cagctggtcc gaagagacaa acgagatctt
      781 ggtctcgcta acacagtcgg gcagctcggt tacgaaaagc cgctggcggt ccatcactcc
      841 accctgctgc gtcaagaaat atgggatcgt cccaccgatc caaacacctt tattgagttg
      901 ggtcagagct gactcatccc ctgctatgtg cagacgcttg ccagcgttga tcagatcatt
      961 tgcttccgaa gctgtgtata aattagacat tataatcccc tatcgactaa tctgcgctaa
     1021 gtccgcggaa gtcagcctct catattttgt aaaaaactca tggagcgttg aaataagctc
     1081 atgtttatct gattgatttt gtgcgtagtc acgcgcagcg agatcgactt tgaggtttgc
     1141 ggcgaatagt gagaatttat gttgcacttt ccgggtcaat agcttttccc atatcgggtc
     1201 tgtgaccggg gcggtaagac tcatccctgc gctccttatc ggcggttggc ggtccgggaa
     1261 aggcgaggca ccagtataat cgtgcaacct gtccgggcgg cgaagggaaa gatcccacgc
     1321 cgagcttacc taagactgaa aaggcaagat cgctttagaa ttgtgtgccg gaaatgaaag
     1381 ctaacggatc ggtattgccg ctatcggacg ataccggccc ggcctgggcc cgaatttcct
     1441 gacgctgtat gcagtatcgt aagaattgcc tggctctatg cttggctgac gctgagatat
     1501 gtcagcttga gcgctatatg agcgacattc gctcagaagc caccggcttg agggcggttc
     1561 cacttggagg gatgaaaaga aagagagaga cgagaccgat ggctagcggg ggggtggggg
     1621 ccatgcaggt ggcctcgtct ctcaggggca acttctaccg ggatcagcgt cccttagtgt
     1681 ccaagactgt ataccaaaac atgtaaacca agcgttacgc gatctttcgg gtctccgaag
     1741 ggcggtttag cgcgacgcaa gactggccgg gcgccaactc atactcaaca gacgcaacct
     1801 acactcgaca acgcaacaat aactccaaca accaactcac taagacgcaa aacttatttc
     1861 aactctactc acggccggcg atttgttctg tggctaggct actttcgccg cctcgtgatg
     1921 tgatatgtat gtcgcagcgg ttagtcgaaa ataaggcgcc aaagcgaaat ttgcttgacg
     1981 cgtgcttttt ccacaatcca caccttttgc gtgcatggtt ctttatataa gttattgtta
     2041 taaattacgt tttcttgggt tggcgtcttg ttttaacgga tgcacaaccc gaagttttcc
     2101 ccagctattt gcttaaaatt gggcgcttct taaaacaaat attaaccttt cgacgctgga
     2161 gcggttcggt gcgcagcgcc gttgcgctga cgcgttttag tactaagtat tgcttacgat
     2221 tagataaatt tgattagtcg gacagaaaag cgggctggca tgagtgagtc gaatcaagcg
     2281 gacgtcatta tcgttggcgc tggtcctgcc gggcttggag ccgcgctcgg atgcctgaag
     2341 gcgggacttg cgcccgccgt cgtggacacc tccaaaagcg cgtccactta cgcttcgaag
     2401 ggcagaagtg ccgcgctctt caacaagacc atcgccttcc tgcagcgtct cggcgtttgg
     2461 gacggctgca aggcgcacgc cgagcccctc aaagcgttgc aattcatcga tgataccgga
     2521 cgccgtctcc gtgctcccga ctgcgtcttt catgccgagg agatcggcga ggccgccttc
     2581 ggctacaaca tcacaaacgc cgacctcgtg gccgctttcc aagccgaggt tgaaaagcag
     2641 aacctgccga tcatcacgcc tgggcctttg accagcttcc gcgaaacggc aaccaaggcg
     2701 tcgttgcgct ttgcggatgg aaccgagctt cgcgctccgc tcgcgatcgc cgcggacggc
     2761 cgcatgtcgg cgacgcgcga ggcggtcgga attcgcaagc tttcatgggc atacgatcag
     2821 attgcaatgg cggcgagctt ctctcatgaa cgcccgcacc gtggggtttg cattgagttt
     2881 caccggagcg cggggccgtt cacgctggtg ccgctgcccg gaaacgcgtc gagttttgtc
     2941 tgggtcgaac gcaagaacga cgccgagcgc ctgcttgcct tggacgatgc cgattttgct
     3001 ttcgagatcg agaaggcgtc gcatctggct ttaggccggg tttcaagtgt cagcgaccgg
     3061 gcgcgctttc cgctgtcctc gcttgccgtt cgagagtatg ggcggacgcg cgtcgccctg
     3121 gtgggagaag cagcgcacgt cactccgccg attggcgctc aggggctcaa tcttggcttt
     3181 cgcgatgtgg aaacgcttat cggtctcctt gccgcggcaa aggagaagaa agaggacctt
     3241 ggctccgacg atttgctcca aagctattcc gccgcccgcc ggggcgacat catgagccgc
     3301 acggttgggg cggacattct caatcggagc ctgctttccg acattctgcc cctgcaagcg
     3361 gcccgcggcg tcggcctgta cgcactcgca gcgattgggc cgctgcgtcg cgccttcatg
     3421 cggcggggaa tggctccggt gggtttcgta tagatcaacc cgcctttaat cggaaaggcg
     3481 gaaagttgat cgattgtaga gtgttagagt agcttatccg cgtcgaaata gacgcgggct
     3541 gctctaatgt cttgacgttc cggccggggc tttgcaagac aggcaggctc gcacgctaac
     3601 cccgcctgga tttatgctgc tcgatatccg ccaacggctc gaata
//
LOCUS       DATMGX010000489         3271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_95811, whole genome shotgun sequence.
ACCESSION   DATMGX010000489 DATMGX010000000
VERSION     DATMGX010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95811"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..561
                     /locus_tag="VNR65_07905"
     CDS             148..561
                     /locus_tag="VNR65_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18634.1"
                     /translation="MKNTGLVRSSVGFLLFALGVATGLTAQTLTDSPQRIEQRRTDLT
                     GAPNMEVIASIVELKPGESSNLHIHHGVEAFHVLQGAMVQAPGQAASMLPTGLTSLNL
                     RDVKHGAFKVVDDHPLKLLTIHIVDKGKPLYDYVN"
     gene            complement(711..1991)
                     /gene="ltrA"
                     /locus_tag="VNR65_07910"
     CDS             complement(711..1991)
                     /gene="ltrA"
                     /locus_tag="VNR65_07910"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HWJ18635.1"
                     /translation="MKQDEANQDLLDAVLDSDNLSRAWKRVKANKGAPGIDGMTIADF
                     PACAREHWPAVREQIIQGRYVPQPVRRVELRKPGGGTRKLGIPVILDRVLQQAIAQVL
                     TPIFDPTFSDSSFGFRPGRNAHQAIRQVQAYVKDGYRIAVDLDLAKFFDTVNQDLLMS
                     LLGRTIADKRLLALIGRYLRAGVLVGEHFEPSEVGTPQGGPASPLLANILLHQLDCEL
                     ARRGHRFARYADDLVILVRSARAGERVMRSITRYLQTSLKLKVNPAKSKVAPMSECSF
                     LSFTIQGNKIRWTDQALAGFKHRVRELTGRSWGVAMEHRLQKLGQYARGWMGYFGISQ
                     YYRPVPLLDDWVRRRVRMCYWKQWRWVRTKIRHLLDLGVSLKTAIQHGVSSKSYWHMA
                     RTPALQQALSNAWLTAHGLVSVKALWSKAQGYTA"
     gene            2699..3226
                     /locus_tag="VNR65_07915"
     CDS             2699..3226
                     /locus_tag="VNR65_07915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ18636.1"
                     /translation="MSYIHRNAVEADLPRIVEIYNFAVATRKCSCDLEPTTVEARLPS
                     FREHTPDHRPFWVAEDSTKPGQGAIGYLGFFHFMNERPGYFITADLAIYLHPDYQGKG
                     LGPYLIQQAISVAPSLGIETLTATIFASNEASIQLFDKMGFERWGFMPRVARLEGVEK
                     DLVLVGKRLFEPRAA"
BASE COUNT          578 a         1092 c          935 g          666 t
ORIGIN      
        1 ccgctgcgcg gcctttggtc tgccgctcat gtcgaacgtt aggtcccatt ggcgcgatgt
       61 acccgagcgc tctacacgac cggcgggttg acaccagagc agtccggcgc gcatagtcgc
      121 ctgcgcacta cgcgcgagga gacttcaatg aagaacacag ggctggttcg gtcctcagtc
      181 ggctttctac tatttgcact cggggttgcg acgggcctta cagcccaaac gctcactgac
      241 tcaccgcaac gaatcgaaca aaggcgaacc gatctcactg gcgctccaaa tatggaggtc
      301 atcgcttcca tagtcgaact aaagccaggc gagagcagca atcttcatat ccaccacgga
      361 gtggaagctt ttcatgtcct gcaaggcgcc atggtgcaag ccccaggcca agccgcctcc
      421 atgctcccaa cgggactgac ttcgctcaac cttcgcgacg tcaagcatgg tgccttcaaa
      481 gtagtcgacg atcatccgtt gaagctcctt acgattcata tcgttgacaa gggcaaacct
      541 ctgtacgact acgtcaacta gcctccgacg cgctggcggc gcatctagcc gcctatggat
      601 gcatggaacc taatcgggta gccgggggtc tttagccccc agcccccaca ccacccaccg
      661 tgcgggtccg cagtgggcgg ttcaacaagt cggctcgcgc gacgaagcag ctatgccgtg
      721 tagccctggg ccttgctcca caaggccttg acgctgacca agccgtgcgc cgtcaaccac
      781 gcgttggaca gggcctgctg cagcgccggg gttcgcgcca tgtgccagta gctcttgctg
      841 ctgacgccgt gctggattgc tgtcttcagg ctcacgccca gatccagcag atgacggatc
      901 ttcgtgcgca cccagcgcca ctgtttccag tagcacattc gaacgcgccg tcggacccag
      961 tcgtccagca gcgggaccgg gcggtagtac tggctgatgc cgaaataccc catccatccc
     1021 cgcgcgtatt gccccagctt ttgcagccgg tgttccatgg cgacgcccca actgcgcccc
     1081 gtgagttcgc gcacccggtg cttgaagccc gccagcgcct gatcggtcca gcggatcttg
     1141 ttgccctgaa tggtgaagct caggaagctg cattcgctca tcggtgcgac cttgctcttg
     1201 gcaggattga ccttgagctt gagggacgtt tgcaggtatc gcgtgatgct gcgcatcacc
     1261 cgctcgccgg cgcgtgcgct gcggaccaga atgaccaggt cgtcggcgta gcgtgcgaag
     1321 cggtggcccc gccgcgccaa ctcgcagtcg agttgatgca gcaggatgtt ggccagcagc
     1381 ggggacgcag gccctccttg cggggtgcca acctcgctgg gctcgaagtg ctcaccgacc
     1441 agcaccccgg cgcgcaggta gcgtccgatc agagccagta gccgtttgtc ggcgatggtc
     1501 cgacccagca aactcatcag caggtcttga ttgaccgtgt cgaagaattt ggccaggtca
     1561 agatccaccg cgatgcggta gccgtccttc acgtacgcct gcacctgacg aatcgcctga
     1621 tgtgcattgc ggcccggtcg aaagccgaag ctcgaatccg agaacgtcgg atcgaagatc
     1681 ggcgtgagca cctgggcgat ggcttgctgg agcaccctgt ccaggatgac gggtattccg
     1741 agcttcctgg tgccgccccc tggcttgcgc agttcaactc tgcgcaccgg ctgcggcaca
     1801 tatcgccctt ggatgatctg ctcgcgcaca gccggccaat gttcgcgcgc gcaggcgggg
     1861 aagtccgcga tggtcatgcc gtcaataccc ggcgcgccct tgttggcctt cacccgtttc
     1921 cacgcccgcg acaggttgtc gctgtccagc acggcatcaa gcaggtcttg gtttgcttcg
     1981 tcttgtttca aggctcggtg cgtgcccgcc cgccgtcaca tctcaccctg tcgggctcgg
     2041 cgatgcgcgc gagggcgaca cgtctgctcc tcgtgtctcg ttgttcagcc cttcagcgac
     2101 ccgactcctg gccacctact atggcgtctg ctgactcctg cgccgtcacg gcgcgcgttg
     2161 ccgcgcgccg cgctgtgttg ccgatggtgg ttgctgccga ttcttcgcat gcagcgggca
     2221 gctcgccgca gcgcctggac cttggtctcc cgggatggtc cgatgccggg ttggtcacca
     2281 tcaacgcgca ccgctcgcgg tgcagatctc cccaggtaag aacgcgaact gtcggtgcac
     2341 aagcgccgca tctaccgtag gctgcgtacc ggtgggcttt gctgtcatgt gccagctcgc
     2401 ctcgcatcca tcggccttga ctatgcggtt tctgtccgtc gcctcgcacc tcttgcactc
     2461 gggcttcctc cggacattcc ctcgcgggtc tgcccttgcc gtcggctcgt ggttatcctt
     2521 gctcacgatg agtccaagtc ggtacctcca cacgggactt acaccccata agttcgcgcc
     2581 catgctgggc gcacacccct ccatcgagcg gacgtcccaa aggccgcttc gcgccctttg
     2641 gtccaccgct catgtcgaac gttagaccgc tcgaatccag accgcactcg ccctgcctat
     2701 gtcgtacatc catcgcaacg ctgttgaagc cgatcttcct cgcatcgtcg agatctacaa
     2761 cttcgccgtg gctacacgta agtgcagctg cgatcttgag cctacaacgg tcgaggctcg
     2821 gcttccttca tttagagagc acacgccaga ccatcgaccc ttctgggtcg ccgaagactc
     2881 gacgaagccg ggacaaggtg ccattggcta tctcgggttc tttcacttca tgaatgagcg
     2941 gcccggctac ttcatcaccg ccgaccttgc tatctacctc cacccagact atcaaggcaa
     3001 agggctcgga ccctacctca tccagcaagc catctcggtt gcgccaagcc tgggcatcga
     3061 gacgctcaca gccacgatat ttgcgagcaa cgaagccagt attcaactct ttgacaagat
     3121 gggctttgag cgctggggtt tcatgccacg cgtagcccgc ttggagggcg tagagaagga
     3181 tcttgtcctt gtcggcaagc gcctctttga gccaagagcg gcctaacatg tcggtcaacc
     3241 ggacccgcta cggcaaggcg ccctggccgc g
//
LOCUS       DATMGX010000490         3544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_33960, whole genome shotgun sequence.
ACCESSION   DATMGX010000490 DATMGX010000000
VERSION     DATMGX010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33960"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1989
                     /locus_tag="VNR65_07920"
     CDS             193..1989
                     /locus_tag="VNR65_07920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWJ18637.1"
                     /translation="MWLIIVLAVTFSIVAAVWVGSIARQTVFEQHVRRLSLETEQLSS
                     DLGQALAARLDAVRASGRILREASIADRPNRLKDVFDELLTAYPQLDWIAVADSSGVV
                     LGTNGALQKGINVSAAQWFSTGLKGPGLGVIESADRAQASTATTAALGDLATPVRDEA
                     GVVVGVVTAHLSWRRAAHHLPRLTDEADSPIATRAFVLDRNEIVLVGPKDMRGKPWNG
                     IPRAAKGLAQSSAGPLEAITTVPQFETLPAGQQVLISRAPLSATNEISALGWQVQLSE
                     PNERVYKRADAVAIRIIWVSLCLGALTALVGALSARHLTRRLKNLARSVNDVGEDEAA
                     KIEVPGGVDEVAQLGRAFAKILEDLQEERRELERRVAVRTHEVERLAEESRYAAIVRE
                     RLKIARDLHDTLAHSMMAILSEIRLLRRLHLRDPAALAGELERAEQVAHQGLTEARSA
                     ITQMRANAVREMGLGPALASAFEKFIDHTGLAGEFSADAQAARFGDERGEVFLRIAQE
                     AFRNIERHAMATRVIVKLHMINGTHMELRIQDNGIGFDPQILLPGHYGIIGLREQAEL
                     IGAELTIDSMRNEGTTISVSLPLSPVAFNRTT"
     gene            complement(2304..2654)
                     /locus_tag="VNR65_07925"
     CDS             complement(2304..2654)
                     /locus_tag="VNR65_07925"
                     /inference="COORDINATES: protein motif:HMM:NF022671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HWJ18638.1"
                     /translation="MALAEIELARVRRALDSFMQQRRPPLHIRPKVDLTFRVSGQSVE
                     IFEIRPVWRGPPDEKHELPIAKATFVRARGVWRIFWQRRDLKWHRYEPRPEVKSVEEF
                     ASLVSEDAHACFFG"
     gene            complement(2660..2998)
                     /locus_tag="VNR65_07930"
     CDS             complement(2660..2998)
                     /locus_tag="VNR65_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWJ18639.1"
                     /translation="MTMALTRDFKKTVVARVERDPAFAKALLDEAATLFLSGEPETAR
                     LILRDLVNATLGFERLAKVTAMPSKSLHRMLSPKGNPNMDNLAAIFGVVRKHLKVDLK
                     AHTVKLQKVA"
     gene            complement(3055..3345)
                     /locus_tag="VNR65_07935"
     CDS             complement(3055..3345)
                     /locus_tag="VNR65_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWJ18640.1"
                     /translation="MEEYIRPDGTIPYKDWFDSLDAQATAKVATAKLRMELGNTSNAK
                     WFAGFGEYVIDWGPGYRIYLAKDGESLIVLFGGGTKRRQQADIDRAQALHAE"
BASE COUNT          763 a         1014 c          996 g          771 t
ORIGIN      
        1 gtagcgcgcc ataaccacga accgtggcgg agaaaacttc cactaagtgt aatcatgcag
       61 ctcgtgtacc atcgtttgga agtgacttta gtcacgactt gagcggaccc catggccaac
      121 tcagttaccg ctgccaaact gagattattc ctctggacta tcgacccacg tcgaagtctg
      181 gaatccggca ccatgtggtt gataatcgtc ttggcggtga cgttttccat tgtggcagct
      241 gtctgggtcg gaagcattgc ccgacaaacc gtgtttgagc agcatgtacg gcgtttgtcg
      301 cttgaaacgg agcagctgag ttcggatttg ggccaagcac tggctgcacg cctcgatgcc
      361 gttcgcgcga gcgggcgcat tttacgagag gccagcatcg ccgaccgacc caataggctg
      421 aaggatgtct tcgatgaact actgaccgct tacccacagc tcgactggat tgccgtagcg
      481 gactccagcg gcgtggtttt aggcaccaat ggcgcgttgc agaagggaat caacgtcagc
      541 gcagctcaat ggttctcgac gggacttaag gggcctgggc tcggggtcat cgagagcgct
      601 gaccgcgcgc aggcgtcaac cgcgaccacc gctgcgctcg gcgacctcgc cacgccggtg
      661 cgcgacgagg cgggagtcgt cgtcggtgtc gttacggccc acttgagttg gcggcgcgcg
      721 gcccatcacc ttccgcgtct taccgacgaa gcggactccc caatcgccac tcgagccttc
      781 gtcctcgacc gcaacgagat cgtgctcgtg ggacccaaag acatgagggg caaaccttgg
      841 aacggcattc caagggcggc aaaaggtctt gcccaatcgt cggccggacc attggaagca
      901 ataaccaccg ttccgcaatt tgaaacgctt ccggccggtc agcaagtcct gatatcacgc
      961 gcaccgttga gcgccaccaa cgagatatcc gcgctcggct ggcaagttca actgagcgag
     1021 cccaatgaac gcgtgtacaa gcgcgcagac gccgtggcaa ttcgcatcat atgggtttca
     1081 ttatgccttg gtgccctcac cgcactggtg ggcgcattga gcgcgcgcca tctcactcga
     1141 cgactgaaga accttgcccg ctccgtcaat gacgtgggag aagacgaggc agccaaaatc
     1201 gaggttcccg gcggcgttga tgaggtcgcg cagctcggac gcgcattcgc caagattctg
     1261 gaggaccttc aagaagaacg gcgcgagctc gagcgacgcg tcgcggtgag gacccatgaa
     1321 gtggagcgcc ttgcggagga atcgcgctat gcggcgattg tacgggaacg gctgaagatc
     1381 gctcgcgatc tgcacgatac gctcgcccat tcgatgatgg cgatactgag tgagattcga
     1441 ttgcttcgaa ggctccactt gcgcgatccc gctgctctgg ccggcgaatt ggaacgtgcc
     1501 gaacaagttg cccaccaggg cctgaccgaa gcgcgcagtg caattactca aatgcgcgca
     1561 aatgcggtgc gcgaaatggg cctaggtccg gcgctggcca gtgcctttga aaagttcatc
     1621 gatcataccg gtttggccgg tgaattcagc gcggatgcac aggctgcgcg gtttggcgat
     1681 gagcgcggcg aggtcttctt acggattgca caggaggcgt ttcggaacat cgagcggcac
     1741 gcaatggcaa cgcgggtgat cgtcaaattg cacatgatca atggcactca catggaatta
     1801 cgcatccagg acaacggtat tggcttcgat cctcaaatac tcctccccgg tcactacggt
     1861 attatcgggc ttcgcgaaca agcggagctc atcggagccg agttgactat tgacagcatg
     1921 cgcaatgagg gcacgacgat ttccgtatca ctgccactat cgccggtcgc tttcaatcga
     1981 accacgtaaa acggccaaaa acctcgcccg caaggacctg caccgcggaa cgtatcgtga
     2041 tgggctgcat tatcatgggt aattggcatg gacgttccgc tgagggattt aggttttgga
     2101 gatggagaaa atggtgcgtc ggtacccgca cctggccgta ggacatctcg cggtctacgc
     2161 cgatgccctg aaagttcatg gcacaatcct ggcacactca atctgagttg gtcacaaatg
     2221 tggcacagcg cagtctcgaa ctatggagct tcacgtgagg gagagatgat gtaatttgaa
     2281 tcaggcaccg catcactccc cgctcatccg aagaagcacg catgcgcgtc ctcggatacg
     2341 agcgacgcaa attcctcgac ggatttcact tccggtcttg gttcgtagcg gtgccacttc
     2401 agatcgcgac gctgccaaaa tattcgccag acgccccgcg ctctgacgaa tgtcgccttg
     2461 gcaatgggta attcgtgttt ctcgtcggga ggtccccgcc agacgggtcg gatctcgaag
     2521 atttctacac tttgccctga cacgcggaag gtcagatcca ccttcgggcg aatgtgtagc
     2581 gggggtcgac gctgctgcat gaaggagtcc aacgccctgc gcactcgcgc cagctcgatc
     2641 tctgcgagcg ccacagtcgc tacgccactt tttgcaactt gaccgtatgg gctttgagat
     2701 ccactttgag gtgttttctt actacgccga aaattgccgc caagttatcc atgtttggat
     2761 tgcctttggg cgacagcatc cgatgaagac ttttactcgg catggctgtt accttggcca
     2821 gtcgttcaaa gcccaaggtt gcattcacga gatcgcgcag aatgagacgt gccgtctccg
     2881 gctcgccact caaaaacaga gttgccgcct catcgaggag cgctttagcg aacgcgggat
     2941 cgcgctcgac tctagcaacg actgtcttct tgaaatctcg agtaagcgcc atggtcatta
     3001 cctctttcgc ttcttggctt tattgccttc cgcagcaggc gctttccgtg ccttctactc
     3061 ggcgtgtaga gcctgcgccc gatcaatatc tgcttgctgc cgtcgcttgg taccaccgcc
     3121 aaacaacaca atcaacgatt caccgtcttt cgcaagatag atccgataac ccgggcccca
     3181 atcgatgacg tattcgccga aacccgcgaa ccacttcgca ttcgaagtgt ttccaagttc
     3241 catgcgcagc ttcgcagttg cgaccttcgc cgtcgcttgc gcgtcaaggc tatcgaacca
     3301 atccttgtac ggaatcgtgc cgtccgggcg aatgtattct tccacccgaa tcttcattat
     3361 tgatagtaac gtttacgtta ctatatagca agcgtcgcgg cttataaggt cgccgtcaac
     3421 gaagcatgca atttagttct gccacctaca gaaagtggac ctagacacgc ggacttttcg
     3481 tccaagtgtc ggggtggctt tggcgggtcg cgagatcgtg taagatattg tttttattga
     3541 aaat
//
LOCUS       DATMGX010000491         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_428623, whole genome shotgun sequence.
ACCESSION   DATMGX010000491 DATMGX010000000
VERSION     DATMGX010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428623"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..1876
                     /locus_tag="VNR65_07940"
     CDS             383..1876
                     /locus_tag="VNR65_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HWJ18641.1"
                     /translation="MTSSEAAALIPVGSNVGMSGFTGSGYPKAVPAALAERINALRTA
                     GEKFKISIWTGASTAPELDGALASVDGIEMRLPYQSDPTCRAKINSGQMEYIDIHLSQ
                     VAQFVWFGFFGKLDVSIVEVGGILEDGRLKPATSIGNNKTWLEQADKVILEVNSWVNP
                     GLEGMHDIYYGTRLPPHRLPIPLVEVSDRIGEKYFRCDPDKVIAVVETHASDRNSKFS
                     APDDRSHRIAGHILEFLAYEVKKGRMPPGLLPLQSGVGNVANAVMAGLEHASYENLTA
                     YTEVLQDGMMHLIKQGKMTFASATALSLSPDASEEFNSDIAFYRDKIILRPQEISNHP
                     EIIRRLGIIAMNGLIEADIYGNVNSTHILGSSIMNGIGGSGDFARNAYLSIFMTPSVA
                     KGGHVSCIVPMASHVDHTEHDVQIIVTEQGLADLRGLSPKQRAKVIIKNCSHPDFRPA
                     LEEYYDRALHYSPGQHTPHLLDEAYTFLGNWKANKEASEAWGARGQF"
     gene            1997..2188
                     /locus_tag="VNR65_07945"
     CDS             1997..2188
                     /locus_tag="VNR65_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18642.1"
                     /translation="MDQAQVLSRQERFKLALEKFCRAHIVDDDPYDALENERFEALIS
                     ARQAAEKDASAIEDLPLAA"
BASE COUNT          506 a          677 c          652 g          470 t
ORIGIN      
        1 aacgtagccc aggcagcccg cgacgactac gccgccgaac aggctgcact tgcggcgcag
       61 gccgcgcttg ctgccgaggc actattcggc agcgacgagg agccggcgga gagccccgcc
      121 tcttaacgaa cttcaaacat atgcgcgagg gtcattcctc gcgcgttccg cgccgagcgc
      181 tgcattattt gcatggcagc cccccaaacc cccggctgcg tcagcgcatt ccttgtagcg
      241 cgaccccaat aaagcttagc ctttgcaact gcggattacc ttcgttgacg cgaggctaat
      301 tgcgcggttt cataacatta gcaaagaggg tcccgccgtg gaaggttcgc tcgtgctcaa
      361 tcctgctctc cggagcaaga ttatgacgtc ttcggaggca gccgccctca ttcctgtggg
      421 ctcgaacgtt ggcatgagcg ggttcacagg ctcgggttat ccgaaagcgg tgcccgccgc
      481 gctcgccgag cggatcaatg cgctacgaac agctggcgaa aagttcaaga tcagcatctg
      541 gaccggggcc tcgaccgcgc cggagctcga cggggcactc gccagcgtcg acggcataga
      601 gatgcgtctg ccctatcaat cggacccgac ctgccgcgcc aaaatcaatt cgggccagat
      661 ggagtatatc gatatccatc tttcgcaggt cgcgcagttt gtgtggttcg gctttttcgg
      721 caagctggac gtttccattg tcgaggtcgg gggcattctg gaagatgggc ggctcaagcc
      781 agccacctcc atcggtaaca acaagacgtg gctcgaacaa gccgacaagg tgatcctcga
      841 agtcaattcc tgggtgaatc ccggcctcga gggcatgcat gacatctatt atggcacgcg
      901 attgccaccg caccgcctgc cgatcccgct cgtggaagtc agcgaccgca ttggtgagaa
      961 atacttccgc tgcgatcccg acaaggtcat cgccgtagtc gagactcatg ccagcgaccg
     1021 caattccaag ttctcggcgc ccgatgatag atcgcaccga atcgccggcc atattctcga
     1081 atttctggct tacgaggtga agaaggggcg catgccgccc ggcctcttgc ctctgcaatc
     1141 cggcgttgga aacgtcgcaa acgccgtgat ggctgggctt gagcacgcct cgtatgagaa
     1201 tctcacggcc tataccgagg tgctgcagga cggcatgatg cacctcatca agcagggcaa
     1261 aatgacgttt gcctcggcga cggcgctttc gttaagcccg gacgcatccg aggagtttaa
     1321 cagcgacatc gcgttctacc gggacaagat catcttgcgc ccgcaagaaa tcagcaatca
     1381 ccctgagatc atacgccgcc tcggcatcat cgccatgaac gggctaatcg aggccgatat
     1441 ctacggcaat gtgaattcga cccacattct cggctcgagc atcatgaacg gcatcggcgg
     1501 ctcgggcgat ttcgcgcgca acgcttatct ctcgatcttc atgactcctt ctgttgcgaa
     1561 gggcggacac gtttcctgca tcgtgcccat ggcaagccat gtcgatcaca ccgagcacga
     1621 cgttcagatc atcgtcaccg agcaggggct cgccgatttg cgcgggctct cgccaaaaca
     1681 acgggcgaaa gtgattatca agaattgctc gcaccccgat ttcaggccgg ccctcgaaga
     1741 gtattacgat cgcgcgctcc attattcccc cggacagcat acgccgcacc ttctcgatga
     1801 ggcctacacc ttcctgggta actggaaagc gaataaggaa gcatccgaag cctggggtgc
     1861 acgaggccag ttctaaaggg ccagaagtgc ccgttctgag agatagcgct caattgcgct
     1921 atctctggta gcatagcgtt agaaatttcg gcttaccgta gctctaggtg aagtttcgag
     1981 tcacgggagc cacgttatgg atcaagctca agttctttcc aggcaggaga gatttaagct
     2041 ggcgctcgaa aagttctgcc gtgcgcatat tgttgatgac gatccgtatg atgcgttgga
     2101 gaacgagcgc ttcgaagcgc tgattagtgc gaggcaagcc gcagaaaagg acgcgagtgc
     2161 aatcgaagat cttccgctcg ccgcctagaa ctgcgcgcgg acgccgataa gctgccctag
     2221 cactttagaa tagatcaact ttttcgcctt taatcatttc gacaaaagcc gggttgatgg
     2281 gggagttggg caaccaagat ggcgg
//
LOCUS       DATMGX010000492         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_100709, whole genome shotgun sequence.
ACCESSION   DATMGX010000492 DATMGX010000000
VERSION     DATMGX010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100709"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(572..2167)
                     /locus_tag="VNR65_07950"
     CDS             complement(572..2167)
                     /locus_tag="VNR65_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ18643.1"
                     /translation="MTRFHSGDENRLRAGQMLKRRIAAFSIATIVAIGAIVAEAVLNE
                     REAAIDRASAEAAILAARFEEQIRSTLDPAPDLKRRMESGAATLGLEEWKSVARELLN
                     NQQKALLGETAAVDLIRSNGVRLARYTAAGGVDTAREIPAAMLVAAQTASDGAYYSEA
                     ATDGVARLYRWRKVLDYPLIIAVGLGRDEVLAPANRQARIVIGLGIAALSLPLIMMVM
                     LGREISSRVESAIALDEESEKVRQEHTALLSISEELAHERIKLRRMNVELIREKRRAE
                     EANQAKSIFLANMSHELRTPLNAILGFSEIIRDKLLGKDVDRYAAYAADIHRSGSHLL
                     GVVNDILDVTRIEAGKLELREERVTVTALIHRCVITLRELAASSDVHLEGPAHDLGAS
                     IYGDKTKLKQILINLLSNAIKFTPPGGRVEIGAEAEPDGDGLCFWVRDTGIGMTGDEI
                     RQALELFRQVDNSLSRRFEGAGLGLPLAVKLTELHGGRLEVRSSPRRGTTVSVYLPAS
                     RIIWDSATMPKPQMEDVPFKYAS"
BASE COUNT          436 a          675 c          620 g          503 t
ORIGIN      
        1 gccaccgggc cggcggtttc gcacgcggtg gatctgcagg aggcggccac ttccgcagat
       61 aacaggctct cctacttcgg cagttcttag ggttttcggg ctttacagag ccttcgcaac
      121 gcgggtcgga gttccggcca gccgaaaagt cggcccgcgc tcgcccggac cttataccaa
      181 ccgcaaaatt tgctgactcg atttcggagg acagacagct cgacaggcct cggagagagc
      241 gcaatacgct cgcgggcatc ggacatcggc cgtgtcgtaa atccgaactc gtttaatatc
      301 tctccaaaac ttcagcagcg tatttcggcc atgtctgctc atgaggtttt ccggcatttt
      361 gttcggaatt ctggaacggc aggaaacggc ctatttcgta catccgttgg tcaagccgga
      421 tggttcgcct agagactatc cgcttttgat gaaatcaaaa agcgggctct aagtttttgt
      481 tttgtcgtgc gtctttatcg gaaaaccgct tcgcactttt ccggacgcag tctagccgcg
      541 atcttttcgt cgtggccggg cttgaccaag ctcaggatgc gtatttgaaa ggaacgtctt
      601 ccatctgcgg cttcggcatc gtggcagaat cccagatgat ccggctggca ggcaaataaa
      661 ccgaaaccgt cgttccccgg cgcggcgagc ttcgaacctc aagccttccc ccgtgcagtt
      721 ccgtgagctt cacggcaagg ggcagtccca ggcccgcgcc ttcgaatcgg cgggaaaggc
      781 tgttatcgac ctgccggaag agttcgagcg cctggcggat ctcgtcgccg gtcattccga
      841 tccctgtgtc ccggacccaa aaacacagcc catctccatc gggctcggcc tccgcgccaa
      901 tctcgacccg gccccctgga ggcgtgaatt tgatcgcgtt ggacaggaga ttgatcagga
      961 tctgcttgag cttggtcttg tctccataga tcgaggcgcc gagatcgtgc gccgggcctt
     1021 caagatgcac atcgctggag gcagccaact ccctcagcgt aatcacacat cgatgaatga
     1081 gcgccgttac cgtgactcgc tcttcccgca actcgagctt tcccgcctct atccgcgtga
     1141 catcgagaat gtcgttaacg acgccaagga ggtgcgatcc cgagcgatga atatcggctg
     1201 cgtaggctgc gtaccggtcg acatccttgc cgagaagctt gtcgcgaatg atttccgaaa
     1261 accccaggat cgcattcagc ggcgtcctca gttcgtggct catgttcgcc aggaaaatgg
     1321 acttcgcctg gttcgcctcc tctgctcggc gcttttcccg gataagctcg acattcatcc
     1381 ttcgaagctt gatccgctca tgggcaagct cttcgctgat cgaaagcaag gccgtgtgtt
     1441 cctgccggac tttctcgctt tcctcgtcga gcgcaatggc gctttcaacg cggctcgaaa
     1501 tctcgcggcc aagcatgacc atcatgatca ggggaaggct gagagcagcg ataccaaggc
     1561 cgattacgat cctcgcctgc cggtttgcgg gagcaagaac ctcgtctctc cctagaccga
     1621 ccgctatgat caaaggataa tcgaggactt ttcgccagcg ataaagccgc gcgaccccat
     1681 cggtcgcagc ttccgagtaa taagccccat ctgacgccgt ttgcgccgcg acgagcatgg
     1741 cggcaggaat ttcccttgcg gtatcgacgc ctcccgcggc tgtgtagcga gcaagtctca
     1801 cgccattgga tcggataaga tcgactgccg cggtttcgcc aagaagggcc ttttgctgat
     1861 tgttgaggag ctcacgagcg acgctcttcc actcttccag gccgagcgtg gcggctccgc
     1921 tctccatcct tcgtttcaaa tccggggccg gatcgagggt gcttcgaatt tgctcctcga
     1981 atcgcgcggc caaaatcgcg gcctcggcgc ttgcgcgatc gatcgcggct tcgcgttcat
     2041 tgagcactgc ctcggcgacg atcgcgccaa tcgcgactat ggtcgcaatg ctgaaagcgg
     2101 ctatacggcg tttcagcatc tgaccggccc tgagccggtt ttcgtcgcct gagtggaatc
     2161 gcgtcattcg atgcacggaa tatacggacc taactgtgtg tcgattgtca gttcgcaaac
     2221 aagctcaatt gcta
//
LOCUS       DATMGX010000493         3235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_204895, whole genome shotgun sequence.
ACCESSION   DATMGX010000493 DATMGX010000000
VERSION     DATMGX010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204895"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VNR65_07955"
     CDS             complement(<1..346)
                     /locus_tag="VNR65_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18644.1"
                     /translation="MGSPVTIALDAMGGDFGPSVVIPAAALSLIRHPDTHYIIFGVES
                     EIRAHMSADSPLASVSKIVHTDVAVAMDEKPSQALRHGRWKSSMWLALEAVKKGEAQV
                     AISAGNTGALMAM"
     gene            complement(475..999)
                     /locus_tag="VNR65_07960"
     CDS             complement(475..999)
                     /locus_tag="VNR65_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18645.1"
                     /translation="MSNPPLQWFIDVHSVHAPLSPSYEANKHELEALKRYAEVEDLTS
                     FSSELRVSPLSAGKFKVAGRLKADAVQASIVDLSAVPAAIDETFSVEFWPQELIDENT
                     GDDVSLGDEPPEPIVGGRIAIGEFLSELFSVSLNPYPRNSDDAFSWEPAEKEPRVTPF
                     AELARLRQKKSDGG"
     gene            complement(1018..1569)
                     /locus_tag="VNR65_07965"
     CDS             complement(1018..1569)
                     /locus_tag="VNR65_07965"
                     /inference="COORDINATES: protein motif:HMM:NF015914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HWJ18646.1"
                     /translation="MFRWFAERTARHEAAEKIYDSVVAQSRNPVFYVRCGVPDTLSGR
                     FDMLVIHMFIVMQNLKLGANEGKLLGKEIIEAFVREMDSMVRDLGIADVYVAQEVRKI
                     ADLFYRQLVVYTNAVEQENRGALAEAIWKSFQSGDENANVAADELATYIVQSIKNIGE
                     MPLNLLLQGHLNFPEIHGLKQAV"
     gene            1717..2217
                     /gene="bamE"
                     /locus_tag="VNR65_07970"
     CDS             1717..2217
                     /gene="bamE"
                     /locus_tag="VNR65_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HWJ18647.1"
                     /translation="MSNQSSKDLGLQRLQEGRRNVSAVFVAGCLLLTACGGHVIRQGH
                     QFQEEDLKQVRDGMSKEQVTLALGTPDTQSAVSGGAYYYISTTAEQPMAFMTPSVTER
                     HVVAVYFDKQNRVEKIANYGLKDGVVVDAVSRETPSYTRDQGILKEIFRNIGASPAIP
                     GMNRGG"
     gene            complement(2256..>3235)
                     /locus_tag="VNR65_07975"
     CDS             complement(2256..>3235)
                     /locus_tag="VNR65_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18648.1"
                     /translation="LATQAAEMRAFRLLVSRLADFKSGPRVPDLPAEQIERLVSDIDV
                     RGEGVSGTSYVATFGVNFSERSIVGLLRQYGVHPIVDRGPEILIIPVFVEDGTAKSSD
                     RNPWRSALADLDLAHAPIPGKLAPTRGDLTAAIAKTYFANPAAGVDTLKAQYKTTQIL
                     MALAELDGGGDNLTVKLVGSDAVGLFSLQREVKARDGIDQAALESAAQLAFETVQERW
                     KITRGSPAVATAAASSNETSEAPPAGGGELVSVQVTAEFSGLKEWQAIRSRLQGVPGV
                     QHWDLRSVNPRSAEIGFDFPGGAERLAELAEARGLSVESGPEGLVVRSR"
BASE COUNT          683 a          964 c          913 g          675 t
ORIGIN      
        1 ccatcgccat gagcgcaccg gtgttgcccg ccgatatcgc aacctgcgcc tcgcctttct
       61 tgaccgcctc aagcgcaagc cacatgctgc ttttccagcg gccatgccga agagcctgac
      121 tcggcttttc gtccatcgcg acagcgacat cggtgtgaac gattttgctc acgctcgcga
      181 gtggcgaatc cgccgacatg tgggcgcgaa tctctgactc aaccccaaag atgatgtaat
      241 gggtgtcggg atggcggatg agcgaaaggg ccgcggccgg aatgaccacg gacgggccaa
      301 aatctccgcc catcgcgtct agcgcaattg taactggcga ccccatgcat aaaaccagaa
      361 agcccgcagg cgtttagagc gcttgcctag cggcagcgcc gactgagcag agaacatact
      421 ggataaaggt tttgtgacaa cgaaaaagat tgcggccaat gaccctgcgt cttctcatcc
      481 cccatccgac tttttttgcc gcagcctggc cagttccgcg aacggagtga cacgcggctc
      541 tttctctgcc ggttcccacg aaaacgcgtc gtccgaattt cgcggatagg ggttgagcga
      601 gacggagaaa agttcggaaa ggaactcacc gatcgctatc cgtcctccta cgataggctc
      661 gggcggctcg tccccgagtg aaacatcatc gccggtgttc tcgtcgataa gctcctgcgg
      721 ccagaactcg acactgaaag tttcatcgat cgcggccgga acggcgctca agtcgacgat
      781 actcgcctgt acggcgtccg ccttcagcct ccccgcgact ttgaatttcc cggctgacag
      841 cggcgacact ctcaactctg aactgaagct tgtgaggtct tcaacctcgg catagcgctt
      901 cagcgcctcg agctcatgct tgttggcctc gtaggatgga ctcaatggcg catgaaccga
      961 atgcacatcg ataaaccatt ggagcggggg attgctcatg gacgcctttc aatgccgtca
     1021 cacggcttgt tttaacccat ggatttccgg aaagttcaga tgcccctgca ggagaaggtt
     1081 taagggcatc tctccgatat tcttgatgga ctgaacaata taagtggcca gctcgtcagc
     1141 agccacattc gcgttctcgt ctccggattg aaaactcttc cagattgctt cggccagagc
     1201 accccggttc tcctgctcga cggcgttggt atagacgaca agctggcggt aaaaaagatc
     1261 ggcgatcttg cgaacttcct gtgcgacgta aacatcggcg atgccgagat cacgaaccat
     1321 actgtccatc tcgcgcacaa aggcctcgat gatttctttg ccgagcagtt ttccttcgtt
     1381 cgccccgagc ttcaaattct gcatgacgat gaacatgtgg atgacgagca tgtcaaagcg
     1441 ccccgacaat gtatcgggga caccgcaccg cacgtagaaa accggattgc gcgactgcgc
     1501 cactaccgaa tcgtatatct tctcagctgc ctcatggcga gctgtgcgtt ctgcaaacca
     1561 gcggaacatt gttcagcctg aactccagtt gtgcgcttgt tgttggcgac tgtaccttat
     1621 aaagccttaa tcgcatccaa accattgcca ttgtgttgcg gcaaagttag gtcaccgaac
     1681 cgaagcgatg cagcttacat caaatttggg ggccgaatgt caaatcaatc ctctaaggat
     1741 ttgggactgc agcgcttgca ggaggggcgc cgcaacgttt ctgccgtctt tgtcgcgggc
     1801 tgcctccttc tgaccgcctg cggaggccat gtcatacggc aaggtcatca gtttcaggaa
     1861 gaggacctga agcaggtccg ggatggcatg agcaaggagc aggtaacgct cgcgctcgga
     1921 acgcccgata cgcaatcggc ggttagcgga ggcgcttatt actatatctc cacaacggcc
     1981 gagcagccca tggccttcat gacgccgtct gtaaccgaga ggcatgtcgt cgcggtttac
     2041 ttcgataaac agaaccgcgt cgagaagatt gcgaattacg gcttgaaaga cggcgtcgtg
     2101 gtcgatgcag tgagccgcga aaccccgtcc tacacgcgcg atcagggtat cttgaaagaa
     2161 atcttccgca atatcggcgc ctctcccgcc atcccgggca tgaacagagg cggttaagcg
     2221 atctccggca ggatacctcc aattctggtc taagctcagc ggctcctgac gaccagcccc
     2281 tcggggccgc tctcgacgct aagcccgcgt gcctcggcta actccgcaag acgctcagct
     2341 cctccgggaa aatcgaagcc tatctcggcg ctgcgcggat taaccgacct caggtcccag
     2401 tgctggacgc ctggaacgcc ttgcaggcgt gagcggatgg cctgccattc cttgagcccg
     2461 gagaattctg cggtgacctg gaccgagacg agttcgccgc cgccagcagg cggagcctcg
     2521 ctggtttcgt tgctgctcgc ggccgcggtt gcaacagccg gggagccgcg cgtaattttc
     2581 caccgctcct gaaccgtctc gaaggctagc tgcgccgcgg actcgagcgc tgcttggtcg
     2641 atgccgtccc tggctttcac ctcgcgctgc agtgagaaca agccaaccgc atcgctcccg
     2701 acaagtttca cggtgagatt atctccgcca ccatcgagtt ccgccagcgc catgaggatc
     2761 tgcgttgtct tatattgcgc tttcagcgtg tccacgcctg ccgccggatt cgcaaaatag
     2821 gtttttgcaa tcgccgcggt gagatcgccc cgcgtcgggg caagctttcc cggtatggga
     2881 gcatgtgcga ggtcaaggtc tgccaacgcg cttcgccatg gattgcggtc gcttgacttg
     2941 gccgtgccgt cctcgacaaa cactgggatg atgagaattt ctggtccgcg atcgacaatc
     3001 ggatgcacgc catactggcg gagaaggccg acgatggagc gctcggaaaa atttacgccg
     3061 aaggtcgcga cgtaagaggt tcctgatacg ccttcgccac ggacgtcgat gtcggagacg
     3121 agccgctcaa tctgctcggc gggaagatcg ggaacgcggg gtccgctctt aaagtcggcc
     3181 aggcgggaaa caagcaggcg aaaggcgcgc atttcggcgg cttgcgtcgc aagct
//
LOCUS       DATMGX010000494         3688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_273491, whole genome shotgun sequence.
ACCESSION   DATMGX010000494 DATMGX010000000
VERSION     DATMGX010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273491"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VNR65_07980"
     CDS             <1..290
                     /locus_tag="VNR65_07980"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HWJ18649.1"
                     /translation="QLPESWIAAKPVLNMSQAMNFVTTNDIVGGNSGSPVINTTGEIV
                     GLIFDGNIQSLGGDFGFDPAVNRAVAVNVGALREALTKVYHADRIAAELAR"
     gene            363..1385
                     /locus_tag="VNR65_07985"
     CDS             363..1385
                     /locus_tag="VNR65_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18650.1"
                     /translation="MAYCLPLSTQQEQYLFRISGLSEGIVSIVFKKIFKKKKRKIIDR
                     VYDFRFGRFEHQTKAKLLSCLQVNSIMSRAILSQKPFFVTRMGATECRICWNFARASF
                     FNNIHSAKLLFEAQNNSGISSRGDPYLNRFASVYIGALPHADLIGLWDVPGMSPIIEK
                     FGSDDLDYTPLIGLEPWSAILEGQLPWTNALSGKSVLVIHPFAKSIQKQYENRTTITT
                     IRALLPEFELDTLVPPITFAGESNENTWVDNLQALMQATAARDFEVALIGCGAYGLPL
                     GAFIKQLGRKAIHLGGVTQLLFGIKGNRWEKRWPYESLMDDTWVRPSREEKPSEANRV
                     EDACYW"
     gene            1914..2234
                     /locus_tag="VNR65_07990"
     CDS             1914..2234
                     /locus_tag="VNR65_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18651.1"
                     /translation="MRAFALSGLAALAISMLAPATVWAFSTEQASPSGGAAANLADQE
                     DPLRALQDKVDGKHSSQQSGFYFSNGSAQQPFTPYGSRLNQTGTNVPFDYSPMPGFRG
                     QRGD"
     gene            complement(2317..3222)
                     /locus_tag="VNR65_07995"
     CDS             complement(2317..3222)
                     /locus_tag="VNR65_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ18652.1"
                     /translation="MKIPKKILSVDDDPLNRDILTEYLTDGGYEVVEADDGDTALKLL
                     NEVPGIDAIVLDRMMPRLNGMEVLKAVKADARFSDIPVIMQSAASARDQILQGIKAGV
                     YYYLTKPYEDQMLLAIVGAALQDAATKKKLREEVSKHRRVMGLMEQSRFRFRTLEEAR
                     NIAFLVANCFPEPEAAVYGLNEMLINAVEHGNLGITYAEKTQFILEGCLYEEIDRRLA
                     QPSNRNKWAYLSFEADENALRVLIKDQGAGFDWRPYMDISPDRATHPNGRGIATSRLM
                     SFTSVEYQGCGNEVLCTAANRSTRL"
     gene            complement(3395..>3688)
                     /locus_tag="VNR65_08000"
     CDS             complement(3395..>3688)
                     /locus_tag="VNR65_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ18653.1"
                     /translation="SNLPELGPAFHCSVSDNGVGIPEAELDKIFDKFTQSSKTNTGAG
                     GSGLGLAICREIVHLHGGTIWAAVSPSGGASIHFVIPREVVSEKADALLVAMN"
BASE COUNT          818 a          978 c         1005 g          887 t
ORIGIN      
        1 atcaattgcc ggaaagctgg attgccgcga agcccgtgct caacatgagc caggccatga
       61 acttcgtgac caccaacgac attgtcggcg gcaactccgg ctcgcctgtg atcaacacga
      121 cgggcgagat cgtcgggctt attttcgacg ggaacattca atcgctcggc ggcgactttg
      181 ggttcgaccc agccgtcaac cgcgccgtag cggtgaacgt gggagcgctc cgcgaggcgc
      241 tgaccaaagt ctatcatgcg gaccgcatcg cggccgaact cgcaaggtag ctatttgtgc
      301 aagagctaag tttcgcgttt tcgatgcccg caaacaggcc tcgtgacagc gacgagaatt
      361 gcatggctta ctgccttccg ttaagcactc aacaagagca atatttattt cgtataagcg
      421 gtctgagcga gggcattgtg tccatagtat tcaagaaaat atttaagaaa aaaaagcgga
      481 agatcattga cagagtctac gattttcggt tcggtcgatt cgaacaccaa acaaaagcaa
      541 agttgttgtc ttgccttcag gttaacagta ttatgtcccg tgcaatctta tcccaaaagc
      601 cattttttgt cacccgcatg ggagcgactg aatgtcgtat ttgctggaat tttgctcggg
      661 catccttttt taataatatt catagcgcga aattactgtt cgaagcacaa aacaactctg
      721 gtatatcatc gcgtggagat ccatatctga atagatttgc atcagtctat atcggcgcac
      781 ttccacatgc ggatttgatt ggtttatggg acgtgccggg catgagcccg attattgaga
      841 aatttggcag cgatgactta gactatacgc cgctcattgg gttggagccg tggagtgcaa
      901 tattagaagg ccagctgcct tggacgaacg cgctctcggg taagtccgtg cttgtgattc
      961 atccgtttgc taaatctata caaaaacaat acgaaaaccg aacaacgatt acaaccatcc
     1021 gagcgctctt gccagaattc gaacttgata ccttagttcc ccctatcact ttcgctggcg
     1081 agagcaacga gaatacttgg gtcgataatc tccaggcgct gatgcaggca acggcggcac
     1141 gtgattttga ggtggcgctc atcggttgtg gcgcttatgg tcttcctcta ggcgcattta
     1201 ttaaacaatt aggccgaaaa gccattcatc tcgggggtgt gacccagctt ctctttggta
     1261 tcaagggcaa ccgttgggag aaacgctggc cctacgagtc cctcatggat gacacgtggg
     1321 tgcggccctc aagggaagaa aagccctcag aggcgaatcg cgtagaagac gcttgttatt
     1381 ggtagatggg tctcgcgaca cagaggtttt ggcgtcgttg ctgaaataag gggtgaggcg
     1441 ggggcccgtg ggccccaatc tgccttgtct agttaatagt cttggttggc gttgacgcaa
     1501 gccgcagagc aagccattca taggcttggg cgacatcttc gagcgcgttt ttgagccaga
     1561 tttcatccaa ggctttcgca tgcgaccttg tttctttggc gcgccgcatg aggtcatctg
     1621 gacgatagtg tttagaggcc aactcatcct ccttcagtgg taaggagtaa ttctggcatt
     1681 ttgtgcagcg cacaaagttg atttatatca acaatggatt aatgcgcggc gtaagttgcc
     1741 gctgagacac agatataaag cattgattta aaacacgaac tttcggctgc tgacactgtt
     1801 cgccgcaccc atgcccagaa cttgctttag atttcaatgt cttgcctccc ctctgtacga
     1861 atcgggccgc gctatggtag tcagtggaaa agcagttgtt gacgagggaa aaaatgcgcg
     1921 catttgctct gtccggcctt gcggctctgg cgatttcaat gctggcccca gccacggtct
     1981 gggccttctc cacagaacag gccagcccta gcggcggcgc agccgcgaac cttgcggacc
     2041 aagaggatcc gctgagggcg cttcaggata aggttgacgg caagcacagc tcgcaacaga
     2101 gcggcttcta cttttcaaac gggtccgcgc agcagccgtt taccccctat ggctccagat
     2161 tgaaccagac gggtacgaac gtgccgttcg actactcgcc tatgccgggt ttccgtggac
     2221 agcgtggcga ctagagcagc ccgcgcgcct tggacgcaga taagctgctc tagcacttta
     2281 gaatcggtca actttcccgc ctctaatcga ttccgattaa aggcgcgttg atctattcgc
     2341 cgcggtgcat aggacttcgt taccgcaacc ctgatactcg accgaggtga acgacatcag
     2401 ccgcgacgta gcgatgccgc ggccattggg atgggtggcg cggtcggggc tgatgtccat
     2461 atacggccgc caatcgaagc ccgcaccctg atccttgatc agcacgcgaa gcgcattctc
     2521 gtcggcctcg aagctgagat aggcccactt gttccggtta cttggctgcg cgagcctccg
     2581 gtcgatctcc tcatagaggc aaccctcaag aatgaactgc gttttctcgg cataggtgat
     2641 gccaagattg ccgtgctcca cggcattgat cagcatctca ttgaggccgt agacggcagc
     2701 ctctggctcc ggaaagcaat tcgcgaccag aaatgcgatg ttcctcgcct cttccaaggt
     2761 gcggaagcgg aaacgcgatt gctccatgag gcccattact cgccggtgct tgctgacctc
     2821 ctcgcgaagc tttttctttg tcgccgcgtc ttgcagcgct gctcccacga tggcgaggag
     2881 catctggtct tcatagggct tggtgaggta ataatagacg ccagccttga tcccctgcag
     2941 gatctggtcc cgcgcgctcg ccgccgactg catgataacg gggatatcgg agaagcgcgc
     3001 atccgccttc accgctttca gaacctccat gccgttgagg cggggcatca tgcggtcgag
     3061 gacgatagcg tcgatccctg gtacctcgtt cagcagcttg agcgctgtat ccccatcgtc
     3121 tgcttcgacg acttcgtaac cgccgtcagt cagatactcc gtgaggatat ccctgttaag
     3181 cgggtcatcg tctacagaga ggattttctt aggaattttc attattttcg agctctgcga
     3241 gacgtgtgcg ccatttctgc tgtcgttgta aatgtaatta gaatctggaa aaactttgca
     3301 atcttggctt ttataatttg ccgaaagatg aaagcgccag gccttagatc aacccgcctt
     3361 ttgatcggaa ccgcgtccaa atggatgcgg ctgcttagtt catcgcgacg agaagagcat
     3421 cggctttttc gctgacgact tccctcggta tgacgaagtg gatagaagcc ccccccgaag
     3481 gagacacggc cgcccatatg gtgccgccgt ggaggtgaac gatttcgcgg catatcgcga
     3541 ggccgaggcc agagccgccg gcgccggtgt tggtcttgct gctttgggtg aacttatcga
     3601 agatcttgtc caactccgcc tccgggattc cgacgccgtt gtctgaaacg ctgcaatgga
     3661 acgctgggcc aagttcgggc aggttgct
//
LOCUS       DATMGX010000495         3635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_309631, whole genome shotgun sequence.
ACCESSION   DATMGX010000495 DATMGX010000000
VERSION     DATMGX010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309631"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VNR65_08005"
     CDS             <1..663
                     /locus_tag="VNR65_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWJ18654.1"
                     /translation="MGIKTVMITGDNPLTAAAIAAEAGVDDFLAQATPETKLKLIRDE
                     QAQGKLVAMCGDGTNDAPALAQADVGVAMNTGTMAAREAGNMVDLDSDPTKLIEIVEI
                     GKQLLMTRGALTTFSIANDVAKYFAIIPAAFAMTYPELGALNVMHLATPNSAILSAVI
                     FNALVIVVLIPLAIKGVKYRALGAGSLLRRNLLIYGLGGIIVPFPGIKLIDMLLSAAG
                     WA"
     gene            681..1250
                     /gene="kdpC"
                     /locus_tag="VNR65_08010"
     CDS             681..1250
                     /gene="kdpC"
                     /locus_tag="VNR65_08010"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HWJ18655.1"
                     /translation="MRLIRPAVSLLALMTVLLGIVYPLVITGVAKVAFSRQAEGSLIY
                     QNGKLIGSTLIGQSFSDPKYFWGRPSATSPQPYNGLASSGSNLGPLNPALLDAVKANA
                     KALHEADPDNRQPIPVDLVTASASGLDPEISPAGAHYQAVRVARARNLPLAQVEALIG
                     AHEQGRLFALVGERRVNVLELNLALDRMR"
     gene            1353..2552
                     /locus_tag="VNR65_08015"
     CDS             1353..2552
                     /locus_tag="VNR65_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWJ18656.1"
                     /translation="MKKTIISGVVCSVLGASSMTIWADDQPATVTPAAPAGPTAMSTP
                     SMTFPLAANPNPLSLDAGPLGKVYITGALTGLGLTQNNAGPPICNSCDKSSLGDISNG
                     QIFIQKTTGLVQFFLQGGGYSFPTIGQPYVRSGDATNNTFGIFPVGYLKLAPGDGSFS
                     IEAGKLPTLFGAEYSFTFEITNIERGLLWNQENSVTRGVQANYTAGPLAFSLSWNDGY
                     YTKRYNWVTGSAAWTINSANTVSFVAGGNTGTTSLKDDSSVPLAQDNGSMYNVIYTHT
                     AGAWTIQPYLQYQNTPSNESIGIASSAHALGGALLVNYAVNSKFSLPARLEYLGSSGG
                     TNLLYGAGSKAWSVTVTPTYQENIFFARLELSYVAAQSSTAGFVFGETGTNTSQARAM
                     IETGIIF"
     gene            2655..>3635
                     /locus_tag="VNR65_08020"
     CDS             2655..>3635
                     /locus_tag="VNR65_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HWJ18657.1"
                     /translation="MVENERRNPDELLADVQREDARARRGRLRIFFGASAGVGKTYSM
                     LEAARAAKGGGADIVIGYVEPHGRVETERLMEGLEQLPFLEVRYRGITRREFDLDAAL
                     HRAPATILVDELAHSNITDGEPRPRHAKRWQDVEELLDAGISVWTTLNVQHLESLNDV
                     VAGITGIRQQETLPDRIFDEASEVELIDLTPDDLLARLKAGKVYVSGQVGTATERFFR
                     TPNLLALRELALRRTADRVDAAAQEYQGKERASRPWLARDRFLIAVAPDDQAEQLVRF
                     GKRFADALDAEWLVVAVETPDLLKLSERERNRRIDVLRLAESLGAETVTLN"
BASE COUNT          737 a         1084 c         1097 g          717 t
ORIGIN      
        1 atgggcatca agaccgtcat gatcacgggc gacaacccgc tgaccgccgc tgcgattgcc
       61 gccgaagcgg gtgtcgatga ttttctcgcc caggcgacgc cggagacgaa actgaagctc
      121 attcgcgacg agcaggcgca aggcaagctt gtcgccatgt gcggcgatgg caccaatgac
      181 gcgccggccc ttgctcaagc cgatgtcggc gttgccatga acaccggaac gatggcggcg
      241 cgagaagcgg gcaatatggt cgatctcgac agcgatccga caaagcttat cgagatcgtg
      301 gaaatcggca agcagctcct catgacgcgc ggcgccctca cgaccttctc gatcgccaat
      361 gatgtcgcga agtacttcgc cattatcccg gccgcctttg cgatgacgta cccggaactc
      421 ggtgcgctca acgtcatgca cctggcgaca cccaactcgg cgatcctgtc ggcggtgatc
      481 ttcaacgccc tcgtcatcgt cgtgttgatt ccgctcgcca tcaagggcgt gaagtaccgg
      541 gcgctgggcg ccggttctct gttgcggcgg aatctgctca tctatgggct gggtggcatc
      601 atcgtgccgt tccccgggat caaactgatc gacatgctct tgtcggcggc cggctgggca
      661 taaggagatt aaccatgttc gtgcgactca ttcggcccgc ggtcagtctc cttgctttga
      721 tgacggtcct tctcggtatc gtgtatccgc tcgtcatcac cggcgtggcg aaagtagcct
      781 tctcgcgcca agcggaaggt agcctcatct atcaaaacgg caagctcatc ggatccacct
      841 tgatcggaca gagtttttcg gatcccaaat acttctgggg ccggccgtcc gcgaccagcc
      901 ctcagcccta taatggtctc gcctcgtcgg gctcgaactt aggcccgctg aaccccgcat
      961 tgctcgatgc ggtcaaggcg aacgccaaag ccctgcatga ggcagacccc gacaaccgtc
     1021 agccgattcc ggtcgacctc gtgaccgcct cggcgagcgg tctcgacccg gaaatcagcc
     1081 ctgcaggtgc gcattatcag gccgtccggg tggcccgcgc gcgaaatctc ccgctcgcgc
     1141 aggtcgaggc actgatcggg gcgcacgagc agggccgcct gttcgccctt gtcggcgagc
     1201 ggcgagttaa cgttctcgag ctcaatctcg cgctcgatcg gatgcgctga tcgcgcaaag
     1261 ctgccccttg cacgggcgac cgtgacggct acgcgaacgg atcgcgattc aaatccttct
     1321 ggatgccgga agaatacttt agaggactcc tcatgaagaa aaccatcatc tcgggtgttg
     1381 tctgctcggt gctcggtgcc agttccatga ctatatgggc tgatgaccaa ccggcgactg
     1441 ttactcctgc ggctcccgcg gggccgacgg cgatgtcaac gccgtcgatg acctttccgc
     1501 tcgcagccaa cccaaatccc ctttctctcg atgcgggacc gctcggaaag gtgtacatca
     1561 cgggcgctct tacgggtctt ggacttaccc agaataatgc gggtcccccc atttgcaaca
     1621 gctgtgataa aagctcgctc ggggacatca gcaatggtca gatcttcatc cagaaaacaa
     1681 ccgggttggt gcaatttttc ctccagggcg gcggctattc attcccgaca atcggtcaac
     1741 cctacgtgcg ctcgggcgat gcgaccaata ataccttcgg gatttttccg gtcggttacc
     1801 tcaaactggc tccgggcgat ggcagtttct cgattgaggc cggaaaactt ccgacgctgt
     1861 tcggggcgga gtactcattt acattcgaaa tcacgaacat cgaacgtggg ttgctgtgga
     1921 atcaagagaa tagcgtgact cgcggggtgc aggccaacta taccgcgggc ccgctcgctt
     1981 tctcgctttc atggaacgat ggctattaca ccaagagata caactgggtc acgggctcag
     2041 ctgcttggac gatcaatagc gccaacacag tgagttttgt ggccggcggc aataccggga
     2101 caaccagcct caaagacgac agcagtgttc cgttggccca ggataacggg tctatgtaca
     2161 acgtaatcta cacccacacc gcgggcgcct ggaccataca gccctacctg caatatcaaa
     2221 acacgccgag caatgagtcg attggcattg ccagcagcgc gcacgcgttg ggcggcgctt
     2281 tgctcgtgaa ttacgcggta aactccaaat tcagcttacc tgctcgattg gagtatctgg
     2341 gtagcagtgg cgggactaac ttgctctatg gtgcgggcag taaagcctgg tcggtgacgg
     2401 tgacgccaac ctatcaggaa aatattttct tcgcgcgctt ggagctatcc tacgttgcgg
     2461 cacagagctc gacggcggga tttgtcttcg gcgagaccgg caccaacacc tcgcaggccc
     2521 gcgcgatgat tgaaacgggt atcattttct aagttctggg tttgacgaac ccgtgtccac
     2581 gcgatgacag gatgatgaca ggatattgaa tcgcgcacgt tgcgccataa ttaagcggaa
     2641 gctgccgaga aacaatggtc gagaacgagc gccgcaatcc ggacgaactg ctggctgacg
     2701 ttcagcggga ggacgcgcga gcacggcgcg ggcggctgcg aattttcttc ggtgcctccg
     2761 ctggcgtggg caagacctac tcgatgctgg aagcggctcg ggcggcgaag ggcgggggtg
     2821 cggacatcgt catcgggtac gtcgaacccc atggacgcgt cgaaacggag cgtttgatgg
     2881 aagggctgga gcaactgccc ttcctggaag ttcgataccg cggcatcacg cggcgcgaat
     2941 tcgatctcga tgccgcatta catcgggcac cggcgacgat cctggtcgac gagttggcac
     3001 actccaatat caccgacggt gagccgcggc cgaggcatgc caaacgctgg caggacgtcg
     3061 aagaactgct cgatgccggg atcagcgtct ggacgaccct caacgtccag cacctggaaa
     3121 gcctgaacga cgtcgtcgcg ggcatcaccg gcattcgtca acaggagacc cttcccgacc
     3181 ggatattcga cgaagcgagt gaagtcgagc tgatcgattt gacgccggac gatttgctgg
     3241 cgcgcctaaa agcaggcaag gtctatgtat ccggccaggt gggcacggcg accgagcgat
     3301 tctttcggac cccgaatcta ttggcgttgc gcgagctggc gttgcgccgt acggccgacc
     3361 gggtcgatgc ggccgcgcaa gagtatcagg gcaaggaacg cgcatcgcgc ccgtggcttg
     3421 cgcgggatcg gttcctgatt gcggtggccc ccgatgatca ggccgaacag ctggtgcgct
     3481 tcggcaagcg gtttgcggat gcgctcgatg ccgagtggct cgtggtggcg gttgagacgc
     3541 cggacctgct caagctatcc gagcgcgaac gaaaccggcg catcgacgtg ctgcgtcttg
     3601 ccgaatcgct cggcgcagaa acggtcacgc tgaac
//
LOCUS       DATMGX010000496         3821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_427632, whole genome shotgun sequence.
ACCESSION   DATMGX010000496 DATMGX010000000
VERSION     DATMGX010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427632"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VNR65_08025"
     CDS             complement(<1..217)
                     /locus_tag="VNR65_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HWJ18658.1"
                     /translation="MVGFRLKSGDSLVVATHNQGKLREFRDLFDLLGVAVASSGELGV
                     AEPEETGETFEENARLKAVVTAAETGRL"
     gene            complement(227..940)
                     /gene="rph"
                     /locus_tag="VNR65_08030"
     CDS             complement(227..940)
                     /gene="rph"
                     /locus_tag="VNR65_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWJ18659.1"
                     /translation="MRPSKRQAGDLRPVSFQRAFSLHAEGSCLIKFGNTHVLCTASLE
                     ERVPPWMKGQGKGWITAEYGMLPRATSERTRREATTGHQSGRSQEIQRLIGRALRAVV
                     DLQALGERQITVDCDVLQADGGTRTAAITGSWVALHDCFQWMRARDMIRDGVLKDQVA
                     AVSCGIYRGDAVLDLDYAEDSEAEADANFVFTGKGMIVEIQASAERAVFSEERMAELT
                     GHAKKGIGELLNLQKLTIL"
     gene            complement(1266..2633)
                     /locus_tag="VNR65_08035"
     CDS             complement(1266..2633)
                     /locus_tag="VNR65_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HWJ18660.1"
                     /translation="MTDDSSLTFFLPAVRRKKVTAAFDGGRLSSDSGVILLSLAERRR
                     AVARTLAALIDDPRDPAHITHTVEDVLRTRVLAIACGYPDGNDLNWLRSDPAFKLACG
                     RLPETGADLCSQPTISRWENAPTLREIIRLTYALIDIWCGSYTAPPRSVVLDIDDTLD
                     VAHGRQEMAEWNAHYDERCFLPIHIYDTATGRPVVMILRPGKTPSGVEVRGWLRRLIK
                     RIRQHWPVTRITIRGDGHYGRDEAMSWCEDNGVDYIFGFGGNAVLDRLVEGEADDIRT
                     RRAEGKLAILRSYAETRYAAKSWTRERRVAARIEAKESDEDDMLRRGLDVRYVVTSLK
                     SGSAEHIYAAVYCARGQAENLIKQHKAQTASDRTSCRSPLANQFRLVLHTAAYWLLLD
                     VRDHVPSWHPLRQSEFSSIRLHLLKVAGRIVETASRIRVALASCCPEAALFGLVAFRL
                     QQSGP"
     gene            complement(2711..3430)
                     /locus_tag="VNR65_08040"
     CDS             complement(2711..3430)
                     /locus_tag="VNR65_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ18661.1"
                     /translation="MKTITTIGLDIAKSVFQVHGIDAAGKVVIRKQLKRGRVLPFFMK
                     LPPCLVGIEACASSHYWARQLTALGHSVKLMPPAYVKPYVKRQKNDAADAEAICEAVQ
                     RPNMRFVPIKSPEQQAGLALHRTRHLLVRQLTATTNSIRAHLAELGIVAPVGRRGVDA
                     LIALVRSDEDGRIPGLMRACLMTLSLQAQALKEQIRTLDRRLLEWHRSSELSKRLDAI
                     PGVGPALAVLIHPTEESGWRG"
     gene            complement(3545..>3821)
                     /locus_tag="VNR65_08045"
     CDS             complement(3545..>3821)
                     /locus_tag="VNR65_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWJ18662.1"
                     /translation="KYPVQSRLAVNTAEAAADAAIAGLGITRLLCYQVSKAIAERSLT
                     LLMRDHEPSPLPVHLVYTGGRLIPQKLRAFLDFVLPRLKAKLVFDPQ"
BASE COUNT          752 a         1157 c         1158 g          754 t
ORIGIN      
        1 cgagccgccc cgtttccgca gccgtcacca cggccttgag gcgcgcattc tcttcgaagg
       61 tttccccggt ttcctcaggt tcggcgacgc caagctcgcc cgaggaagcg acagcaacac
      121 ccagcagatc gaacaagtcg cggaactccc gcagcttgcc ctgattgtgc gtggcgacga
      181 caaggctgtc gccgctctta agccgaaagc cgaccatgct ctccctttag agtattgtca
      241 gtttctgcag attgagaagt tcaccgatgc ccttttttgc atgcccggtg agttcggcca
      301 tgcgttcctc ggaaaacacg gctctctcgg cgctcgcctg gatttcgacg atcatgccct
      361 tgccggtgaa gacgaaattc gcgtccgcct cggcctcgga gtcttcggca taatccaggt
      421 cgagcaccgc gtcgccccgg taaattccgc acgaaacggc tgcgacctga tctttcaaga
      481 cgccatccct gatcatgtcg cgcgcgcgca tccattggaa gcaatcgtga agcgcgaccc
      541 atgagcccgt tatcgcggct gtccgcgtcc cgccatcagc ctgcagcaca tcgcagtcta
      601 cggtgatttg ccgctcgccg agcgcttgca gatcgacgac agcgcgaaga gcgcggccaa
      661 tcagccgttg gatctcctgg cttcgccccg actggtgccc ggtcgtcgcc tcccggcgcg
      721 ttcgctctga ggtggcgcga ggcagcattc cgtattcggc cgtgatccag cctttgccct
      781 gtcccttcat ccaaggcgga acgcgttcct caaggcttgc cgtgcaaagc acatgggtgt
      841 tcccgaactt gataagacac gatccttcag catggagcga gaaggcacgc tggaagctaa
      901 cagggcgcag atcgcccgcc tgccgtttcg aggggcgcat gtaaacctat atttgattga
      961 gtggcgttcg taataagcct taagtgtcgc gaacactagg ctaggcggca acctcgatca
     1021 acgtctttcc tgcggcgtgt tgcatgacct agttgccgca tgaagcgagc agacgatgcc
     1081 gctggcacaa aatttcgcct cttagccgtt cttatgcgtc tgaaccgaac cactgaggtt
     1141 tgttgcttct gaacacgcct gggctcgccg cgagcgtaat cggctgcggc ctgatcagga
     1201 atgggaacgc tgcttacgcg ttggaggttg gcgatatggg ctccaggggc ggcgcgcccc
     1261 gccgttcatg gcccggactg ttgcagccgg aatgcgacaa gaccgaacag tgcggcctcc
     1321 ggacagcagg atgccaatgc aacacggatg cggcttgccg tctcaacgat gcggccagcg
     1381 accttcagga gatggaggcg gatgctgctg aactcactct gccgcaaggg atgccagctc
     1441 ggaacatgat cgcgcacatc gagcagcagc cagtaagcgg ccgtgtgcag aacgaggcgg
     1501 aattggttgg cgagcggcga ccggcaactc gtgcggtcag aggcggtttg cgccttgtgc
     1561 tgcttgatca gattttcagc ctgtccgcgc gcgcagtaga cggcggcgta gatgtgttcg
     1621 gcgctgccgc ttttcagcga ggtgaccacg taccggacat caagcccgcg gcggagcatg
     1681 tcgtcctcat cgctttcctt cgcttcgatc cgtgctgcga cacggcgttc tctcgtccac
     1741 gatttggcgg cgtagcgcgt ttcggcataa ctgcggagaa tggcgagttt cccctcggcg
     1801 cggcgcgtgc ggatatcgtc tgcctcccct tcaaccagcc ggtcgagcac ggcgttaccg
     1861 ccaaagccga aaatataatc gacgccattg tcctcgcacc agctcatggc ttcgtcgcgg
     1921 ccgtaatggc cgtcgccccg gatggtgatg cgcgtaaccg gccaatgctg ccggatgcgc
     1981 ttgatcaggc ggcgcagcca tccgcggact tctacgccag atggcgtttt gccaggacgc
     2041 aggatcatga cgaccgggcg tccagtcgcg gtgtcgtaaa tgtggatggg cagaaagcag
     2101 cgctcatcgt aatgagcatt ccattccgcc atctcctgac ggccatgcgc gacgtcgagc
     2161 gtatcgtcga tgtccagaac cactgagcgg ggcggcgccg tgtaactccc gcaccagata
     2221 tcgatgaggg cataggtcag ccggatgatc tcccgcagcg taggcgcgtt ctcccaacgc
     2281 gagatggtgg gctgcgaaca caaatccgca cccgtttccg gcagccgccc gcaggcgagc
     2341 ttgaaagcgg gatcggaccg cagccagttg agatcgttac cgtcgggata gccgcaggca
     2401 atcgccagca cgcgcgttct gagcacatcc tcgactgtgt gggtgatatg cgccgggtcg
     2461 cgcggatcgt cgatcagagc ggcaagcgtc ctggctaccg cccggcgacg ctcggcaagc
     2521 gaaagcaaga tcacgccact gtcagaggaa agacggccgc catcgaatgc ggctgtgact
     2581 ttcttgcggc ggacggctgg caagaagaaa gtaagtgaac tatcgtcggt catggcgggt
     2641 gtggcagggc gatttgacgt gcgcagagtg gtctcgacac ccatttccta cgcaagttca
     2701 acaccttgcg ctacccccgc cagccagatt cctccgtcgg atgaataaga acggctaacg
     2761 caggtccaac gccgggaatg gcatccagcc gtttgctgag ctcgcttgat cgatgccact
     2821 cgagaaggcg ccggtcgaga gtccggatct gctccttaag agcttgagcc tgaaggctga
     2881 gcgtcataag gcaggcgcgc atgagaccgg ggatgcgccc atcttcatcg cttctgacaa
     2941 gagcgatcaa ggcatcgacg cctcgtcggc caacgggagc gacgatcccg agttcggcga
     3001 gatgcgctct gatcgagttt gtggtcgcag tcagctgacg gacgagcaga tgccgcgtcc
     3061 gatgcaaagc caagccggct tgctgctcag gagatttaat cggcacgaag cgcatattgg
     3121 gccgctgcac ggcttcgcaa atcgcttcgg catcagccgc gtcgttcttc tggcgtttga
     3181 catacggctt cacgtaggcc ggcggcatga gctttactga atgcccgagc gccgtgagct
     3241 gccgcgccca ataatgggag gaggcgcaag cttcgatgcc aaccaggcac ggcggcaact
     3301 tcataaaaaa cggcagaaca cgaccgcgtt tcaactgctt cctgataacc actttgcctg
     3361 ccgcatcaat gccgtgaacc tggaaaacag acttcgcgat gtcgagaccg atggtcgtga
     3421 tcgttttcat gtggctccct ccgaatcgtg gtcgcctcat cggcaaccgc ttcgttggcg
     3481 cttccgcgcc ggtggaagga gccgtccaca gcatcacatg cggaagtatc ttcaacgctg
     3541 gaatttattg tggatcgaac accagcttcg ctttcaggcg cggcagaaca aaatcaagga
     3601 atgcgcgcag cttttgcgga atgaggcgtc cacctgtgta gacgaggtgg acaggcagcg
     3661 gggatggctc atggtcgcgc ataagaagcg tcaagctccg ctcggcaatg gctttcgaca
     3721 cttgataaca caagagccgg gttatcccga ggccggcaat cgccgcgtcg gctgcggcct
     3781 cggccgtatt gactgcaagg cgcgattgaa cgggatattt c
//
LOCUS       DATMGX010000497         3276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_453965, whole genome shotgun sequence.
ACCESSION   DATMGX010000497 DATMGX010000000
VERSION     DATMGX010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453965"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VNR65_08050"
     CDS             complement(<1..825)
                     /locus_tag="VNR65_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ18663.1"
                     /translation="MPHSRVFCHWFRLMDLQLSPAVDDSAPMSASVRRNDQRIIETSI
                     PARLDALTWSGFHTRVVLALGITWILDGLEVTLAGALSGALKESPTLQFSNFDVGFSN
                     SAYLAGAVCGALGFGWLTDRIGRKKLFFITLALYLTATAATALSWGVASYALFRFLTG
                     AGIGGEYTAINSTIQELVPARYRGWTDLVINGSFWIGAAMGAISAIVLLDPLLIGPDL
                     GWRLAYLTGACLGLVVFVMRMWIPESPRWLIIHGYPNRAQAIVGDIERSAIGKLQDQ"
     gene            984..1364
                     /locus_tag="VNR65_08055"
     CDS             984..1364
                     /locus_tag="VNR65_08055"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HWJ18664.1"
                     /translation="MAGRKTATRKTAGKSTQKPTTRKAGSARGKSAGKKLSPKRWSQR
                     VTEQSDALDLRQGVFKLTDPRKIAASLKRSAERSSRRKAGAYRSALSMLTFYINRAGK
                     GLPKTQRERLERAKGELKRQFGRE"
     gene            complement(2247..2543)
                     /locus_tag="VNR65_08060"
     CDS             complement(2247..2543)
                     /locus_tag="VNR65_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18665.1"
                     /translation="MHTKDALASALRDAGLPLMASRAAEGYYHDYLSPLAMPEKTLCE
                     ELTTVGTRAALELCERVKQGDYEASEEEEEAWASSPDGLAAMQALAAKFNKKRA"
     gene            complement(3056..>3276)
                     /locus_tag="VNR65_08065"
     CDS             complement(3056..>3276)
                     /locus_tag="VNR65_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18666.1"
                     /translation="NPAGCSGGVRSNGEVAGRGKDRDATQQQRRCRADKAPIQLSARQ
                     AVHDRSPNVFRLSAPAPRRHHANQKSDR"
BASE COUNT          668 a          967 c         1030 g          611 t
ORIGIN      
        1 ctggtcctgc agctttccga tcgccgacct ttcgatatcg cccacaatcg cctgcgcgcg
       61 gtttggataa ccgtgaatga tcagccatcg cgggctttcg ggtatccaca tccgcatcac
      121 gaagacgacg agaccgagac aggcgccggt gaggtatgcc agccgccagc cgagatcggg
      181 accgatcaga agcggatcga gcagcacgat cgcgctgatc gcgcccatcg ccgcgccgat
      241 ccagaagctg ccgttgatca cgaggtcggt ccagccgcga tagcgcgccg gcaccagttc
      301 ctggatcgtc gaattgatcg cggtatattc gccgccaatg ccggcgccgg tgaggaatct
      361 gaacaacgcg tagcttgcga caccccagga cagcgcggtg gcggcggtcg cggtgagata
      421 gagcgcgagc gtgatgaaga acagtttctt gcgcccgatc cggtcggtga gccagccgaa
      481 gccgagcgcg ccgcacacgg cgccggcgag ataggcgctg ttggaaaatc cgacatcgaa
      541 attggaaaat tgcagggtcg ggctttcctt cagcgccccc gacagcgcgc cggccagcgt
      601 cacctccagc ccgtccagaa tccaggtgat gccgagcgcc agcacgacgc gggtgtggaa
      661 accgctccag gtgagcgcat cgagacgcgc gggaatgctg gtttcgatga tgcgttggtc
      721 gttgcgcctc acggacgccg acatgggagc gctatcatcg actgccgggc tgagctgcaa
      781 atccatcaat cggaaccaat gacaaaacac ccgggaatgc ggcattccag ggtcttcaag
      841 cgtgaatggg gctcgcctgc caccagggtc aacgcaacgg gtacgatcgg gttcccgcat
      901 ggcggcattg ggaactgttt gggttcgctg ccgttttcgg ttgaaatgat ggaaaacaga
      961 cattgaaacc atgggagttg gcgatggcgg gacgcaagac ggcgacgcgg aagaccgcag
     1021 gaaagagcac gcaaaagccg acaacccgaa aggccggttc ggcgcgcgga aaatccgctg
     1081 gcaaaaagct atcgccaaaa cgctggtcgc agcgcgtgac cgagcaaagc gacgcgctcg
     1141 atctgaggca aggcgtcttc aagctgaccg atccgagaaa gatcgcggcc tcgctgaaac
     1201 gctcggcgga acgcagttcg cgccgcaagg ccggcgccta tcgctcggcg ctctcgatgc
     1261 tgacctttta tatcaaccgt gccggcaagg gcctgccgaa gacacaacgc gagcggctgg
     1321 agcgggccaa gggcgagttg aagcggcagt tcgggaggga gtgaagtgtc atacccgcga
     1381 aggcgggtat ccagtaatcg ccggccccaa gtgagaatcg agacattccg gcgtactgga
     1441 tccgccggag cctgtcgtcc ggccggccag aggccgaacc ggatggcgga tgatgacagc
     1501 aggggtgtgc gtgggatcat tgggatcgtc aagatacgac tccgctatct cgccgcaggt
     1561 tttgcgcgag ttttggcttg cacgttccgc cctctttgat cagagggtgc agggaatgcc
     1621 gggcgcccga tgcgcccgat agccgcgtgt gcaggggtag tgtgagaagc gcacacgcgt
     1681 tagtcaggtc acaccggaat cacccggcat tccctacgca atggtttacg gcttataacg
     1741 cgctctcccc ggtgaccggg ctttcttgcc accgtcgccc acggatcact ccgcgaactt
     1801 gacaccagcg tcggggtgtc aggaccacac gacttcgccg tccgcgtcag cgctgttcgt
     1861 caagagcgct gaaagcgtcc accgcatccc gccccgcgtc cgtgacgatc gcgagccgcc
     1921 cctctgtggg acgggacggc gggggatata gaagtgattt gcgtcttcgg aaaaccagaa
     1981 tatttttgca aagggggctg gacaggcaaa tcactgattt gccggtccgg gcacaaaaag
     2041 cccgccagac acggatagcg agtgcgacgg gatgagcgct tcgcgacacc cagcgtggtt
     2101 gtcccgaaca ggagaccaac tatcgctcgc gccatccgcc ttcgctctgc aagcttcggc
     2161 ggacgcggtc gctcgctatc gcctcaatct tcgcgcatgt ctgccatggc acgtctgcgg
     2221 cctggcaaag ggcttagaag atgtcgtcag gctcgcttct tgttgaattt tgcggcaagc
     2281 gcctgcatgg ccgcgagacc gtcgggactc gacgcccacg cttcttcttc ctcttcgctg
     2341 gcttcatagt ctccctgctt cacgcgttcg caaagctcaa gagcggcccg cgtgccgacc
     2401 gttgtcagtt cttcgcaaag tgtcttttca ggcatcgcga gtggagaaag gtagtcgtga
     2461 taatagccct cagcggctct tgaggccatg agtggcagtc ctgcgtcgcg tagcgcggaa
     2521 gcaagagcgt ccttggtgtg catacgaccc tcccacggtt gaaagtgaga tctgagccaa
     2581 actgctcacg tcgcactgta caaaactggt catcaccaca gttcttttcg ctgcggtatt
     2641 cgaggaagat gacgcgtttg ttgaggcagg aaactgcgct gtctattcct ggccgaaaca
     2701 attattatgt cgccttccat tgtgccgggt atccggcgtc tttgagggcg catcaaaggc
     2761 gcctaacagg cgaatgcgct agggctgggc atcagcattc agggaacctt tcacctcgtg
     2821 agcacattgt tggcaggcca ttggaatggg aatgtcgaaa atgggcgagt tacttgacgc
     2881 gttaaacgcg acggacagcg aggccgccgc accgaccaaa tcggccgtgg atcaagctac
     2941 ggagctcttc cgctttgcga gcaaaaaggt caatgaggca atagatgccg ttcaagagcc
     3001 cggcatgcct cttgacacaa tcagccgatg ggcgcggcaa gctccccttc aagcactagc
     3061 ggtcgctttt ttggttggcg tgatgacggc gcggcgccgg cgccgatagg cgaaagacgt
     3121 tgggagaccg atcatgcacc gcctgacgcg cgctcaattg tatcggagcc ttgtcggcgc
     3181 ggcatcggcg ctgttgctgt gtggcatcgc ggtctttgcc gcggcccgct acttcgccgt
     3241 ttgaacgaac cccgccggag catccagcgg ggtttc
//
LOCUS       DATMGX010000498         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_319490, whole genome shotgun sequence.
ACCESSION   DATMGX010000498 DATMGX010000000
VERSION     DATMGX010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319490"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1132
                     /locus_tag="VNR65_08070"
     CDS             182..1132
                     /locus_tag="VNR65_08070"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ18667.1"
                     /translation="MAVLEQSWSTEHLQPGKALSYWRDVICENLLQMHIHSSQEGAFF
                     GSIEKHAFGPIRANFISVSEQKVWRERTTASKAKDHLFHLIHVRKGVQLIEQYGRSLK
                     VEAGDCLLIDCLAGFEFDFPQGVEALVLEIRRDWLKGWLPAPEDAAARLIDGRSGWGA
                     TLASALSNVTPNTVAASGLPPTVVAEQIVTLLALATPHNDSSPTTHQRALLRRVTETI
                     RERCHEPDLDPATVAAALGLSRRYVHLLFASAGTTFSRELYACRLHRAQRLLCDKRFD
                     GLGIAEIAWNCGFSEPSHFTRRFRERFGSSPSVYRLNSTN"
     gene            1235..1831
                     /locus_tag="VNR65_08075"
     CDS             1235..1831
                     /locus_tag="VNR65_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWJ18668.1"
                     /translation="MALQIHAAWKVRPIHCTLSIQSRNDKLTANRGDPGIKTGILDRL
                     VGYQVHMVDLIALQNSRDALAAHDTTPATVTAMLLIHDRPGCDQATVGRLLSINRSSA
                     AKFIDRLETKGFVKRSAGLDRRSNGLHLTAAGARHLVEVVALLKKVNASLCRTLDVGE
                     QGELLRLLRKILRSARLEQGVEAGKTLGRKLKQKRGKP"
     gene            1828..>2045
                     /locus_tag="VNR65_08080"
     CDS             1828..>2045
                     /locus_tag="VNR65_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWJ18669.1"
                     /translation="MTAENTVKFRVEDRIAWVKFNRPEKRNSMSPSLNRRMMEVLDEL
                     EFRDDVGVLVLGGEGSAWSAGMDLKEYFR"
BASE COUNT          423 a          558 c          648 g          416 t
ORIGIN      
        1 agtttcaagc aacccggaac gccaagatcg agtatggggt gggcgtcaga cgcgacggct
       61 gatgcggggc gaatcgcgtc ttgtatccgg tatctcaaca gagcaagatg ggatccgata
      121 tcgttgcctg ccaattgcac gaaaggccgg gagtaggttg ggattggcgc gggagtgcct
      181 gatggcagta ctcgaacaga gttggtcgac cgaacacctg cagcccggca aggcgctttc
      241 ttattggcgc gatgtgatct gcgagaatct tctgcagatg cacattcaca gttcgcagga
      301 aggtgcgttt ttcgggtcca tcgagaagca cgccttcggt ccaatcaggg cgaatttcat
      361 ttcagtcagc gagcaaaagg tctggcgcga gcgcacgacg gcaagcaaag caaaggacca
      421 cctcttccac ctgatccatg tgcgcaaggg cgtgcaactt atcgagcagt acggccgcag
      481 cctcaaggtc gaggccggcg attgcttgct gatcgattgc ctcgccggct tcgagttcga
      541 ctttccccag ggcgtcgaag ccctggtgct cgagatccga agagattggc tcaagggctg
      601 gctgcccgcg cccgaggacg cggcggcgcg cctcatcgat gggcgtagcg gttggggggc
      661 tacgttggca tcggcgctga gtaacgtcac gcccaatacg gttgcggcat cggggttgcc
      721 gccgacggtg gtggcggaac agatcgtcac tctgttggcg ctcgcgaccc cacacaacga
      781 ttcgtcgccg accacccacc agcgggcgct gttgcgacgt gtgacggaga cgatacgcga
      841 gcgctgccac gagcctgacc ttgatccggc aacggtcgcc gccgcgctcg gtctgtcgcg
      901 tcgttacgtc catctgctgt tcgcctccgc cggcacgacc ttcagccggg aattgtatgc
      961 ctgccggctc cacagggcgc agcggctgct gtgcgacaag cgattcgatg ggctcggaat
     1021 tgccgaaatc gcctggaatt gtgggttcag tgaacccagc cacttcaccc gccgatttcg
     1081 cgaacgcttc ggttcgtcgc cgagcgttta ccggctgaac agcacgaact gacgaaccgc
     1141 tccccggctc tcgtcgcgaa tcatggtgac cattaccact acccatgttg gcagtctgcc
     1201 gcgcggcgcc cgctcaacat gggtcgcccg ttgattggcg ttacaaattc atgcggcatg
     1261 gaaagttcgc ccaatacatt gtacgcttag catacaatct cgtaatgata agctgacagc
     1321 caatcgagga gacccgggca ttaagactgg aatactcgac cgtctggtgg ggtatcaggt
     1381 ccatatggtt gatctgatcg cgctccagaa ttcccgtgat gcgcttgccg cacacgatac
     1441 gaccccggcc acggtcacgg cgatgttgtt aattcacgac cgcccgggtt gtgatcaagc
     1501 gacggtaggg cgtctgcttt cgatcaaccg ttcgagcgcg gcgaaattta tcgacaggct
     1561 tgaaacgaaa ggatttgtga agcgttcggc gggccttgat cggcgaagca acggccttca
     1621 cctcacggcg gctggagccc ggcatttggt cgaggttgtt gcgctgctga agaaggtcaa
     1681 cgccagtttg tgcaggacct tggacgtcgg cgagcaaggg gagctgctgc ggcttttgag
     1741 aaagattctc agaagtgcga ggctcgaaca gggcgtcgag gcaggaaaga cactaggacg
     1801 caaattgaaa cagaagcggg ggaaaccatg actgcagaga atactgttaa atttcgtgtt
     1861 gaggatcgca ttgcctgggt gaaattcaat cgtccggaaa agcgcaactc tatgagtccg
     1921 tcgctcaacc gcagaatgat ggaggtgctt gacgaattgg aatttcgcga cgacgtgggg
     1981 gtgctggtcc tgggcggcga agggtccgcg tggtcggcgg ggatggatct gaaggaatat
     2041 tttcg
//
LOCUS       DATMGX010000499         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_122475, whole genome shotgun sequence.
ACCESSION   DATMGX010000499 DATMGX010000000
VERSION     DATMGX010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122475"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..593)
                     /gene="iscU"
                     /locus_tag="VNR65_08085"
     CDS             complement(180..593)
                     /gene="iscU"
                     /locus_tag="VNR65_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HWJ18670.1"
                     /translation="MSYSDKVIDHYNNPRNVGSMDKQSTDVGTGLVGAPECGDVMKLQ
                     IKVNRETNVIEDAKFKTFGCGSAIASSSLATEWVKGKTVDEALAIKNMDIVKELSLPP
                     VKIHCSVLAEDAIRAAIGDWKKKQEQTSPVAAEAK"
     gene            complement(701..1987)
                     /locus_tag="VNR65_08090"
     CDS             complement(701..1987)
                     /locus_tag="VNR65_08090"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HWJ18671.1"
                     /translation="MSTDVKIPQALTQAHHTEAKGVKLPIYMDNHATTQMDPRVLEEM
                     LPYFIERFGNSASRNHSFGWAAEEGVETARERIAKLIGATTKEIIFTSGATESDNLAI
                     KGVAEMYREKGNHIITAVTEHKAVLDTCKRLEKYGYRVTYLPVQKDGLIDLDDLKRAM
                     DDKTILVSIMYANNEIGVVQPVVEIGKLCHERGVIFHTDATQAIGKVPVDVIKQNIDV
                     MSISGHKMYGPKGVGALYVRRKNPRVQISASIDGGGHERGMRSGTLNVPGIVGLGKAC
                     AIAKEEMAQESCRLAGLRNRLRDRIMNRLDEAYINGSMEHRLPGNLNISFAYVEGESL
                     LMGINDIAVSSGSACTSATLEPSYVLKALGTGDDLAHSSIRFGIGRFNTEAEVDYVAD
                     RVIETVERLRELSPLYEMAKEGINLKDVKWAGEASH"
BASE COUNT          378 a          632 c          624 g          549 t
ORIGIN      
        1 atgccttcct tggccatggc gctgcgcacc ttggccagag ccttctcggt gacgagaatg
       61 cctttggcgg gagcttgcgc gggcggtttt tgttcggttg ctgttgtcat aagtcagcca
      121 tgagctctga gcttcgagcg gcgagcaaac gctcgcagct tatggatcga agctcgtaac
      181 tatttcgcct cggcggctac gggactggtc tgttcctgct tcttcttcca gtcaccgatg
      241 gcggcgcgga tcgcgtcttc ggccagcacg gaacagtgga tcttcacggg aggaagcgaa
      301 agctccttca cgatgtccat gttcttgatg gcgagagctt cgtcgacggt cttgcctttg
      361 acccactcgg tggcgagcga ggacgaggca atggcggagc cgcaaccgaa ggtcttgaac
      421 ttggcgtctt caatgacgtt ggtttcgcgg ttgaccttga tctgcagctt catgacgtcg
      481 ccgcactcgg gcgcgccgac caggcccgtg cccacgtcgg tgctctgctt gtccatggag
      541 ccgacgttgc gggggttgtt gtagtgatcg atcactttgt cgctgtatga catgttttct
      601 ccaaatcgag tttcaaagtt tcaaggtttc agagtttcaa ggtcttcgat ccggttcaag
      661 gagttcagct ttgaaacctt gaaacgttgc aaccttgcac ctagtgcgac gcttctcctg
      721 cccacttcac atctttcaga ttgatgcctt ctttcgccat ttcgtagagc ggagaaagtt
      781 cgcgcagacg ctcgacggtt tcgatgacgc ggtcggcaac gtaatccact tccgcttcag
      841 tgttgaagcg gccgatgccg aagcggatcg agctgtgcgc caagtcgtcg cccgtgccga
      901 gagccttcag tacatacgat ggctccagcg tcgccgaggt gcaggccgaa ccgctcgaaa
      961 ccgcgatatc gttgatgccc atcaggagcg actcgccttc gacgtacgcg aagctgatat
     1021 tcaagttgcc cggcagacga tgttccatcg aaccgttgat gtaggcctca tccagacggt
     1081 tcatgatgcg gtcgcgcagg cgattgcgca ggcccgccag acggcaggac tcctgcgcca
     1141 tttcttcctt agcaatcgcg caagccttac ccaagccgac gattcccgga acgttcaatg
     1201 tgcccgaacg catgccccgc tcgtgcccac cgccatcgat gctcgcggaa atctgcaccc
     1261 gaggattctt gcgccgcacg tacagtgcgc cgacgccctt cggtccgtac attttgtgac
     1321 cggagatcga catcacgtcg atgttctgtt tgatcacatc tacggggacc ttgccgatcg
     1381 cctgggtcgc atcggtatgg aagattacgc cgcgctcgtg acacagcttg ccgatttcga
     1441 caacgggctg caccacgccg atctcgttat tggcatacat gatcgacacc aggatcgtct
     1501 tgtcatccat ggcacgcttc aggtcgtcga gatcgatcag gccgtccttc tggaccggca
     1561 ggtacgtgac acggtagcca tacttctcca accgcttgca ggtgtcgagc acagccttgt
     1621 gttcagtcac cgcagtgatg atgtggttgc ccttttcgcg gtacatctcg gccacgccct
     1681 tgatggcgag attgtcgctc tcggtggcgc cggaggtgaa gatgatttcc tttgtggtcg
     1741 cgccaatgag tttggcgatg cgctcgcgcg ccgtttccac gccctcttcg gcggcccagc
     1801 cgaatgagtg attgcggctg gccgagttgc cgaatctctc gatgaagtag ggcagcattt
     1861 cttccagcac ccgcggatcc atctgggtgg tggcgtggtt atccatatag atgggcaact
     1921 tcacgccctt ggcttcggtg tggtgcgctt gcgtgagtgc ctgggggatc ttgacgtccg
     1981 tgctcattga ttgctctcct gcttcgttaa tgtctttgat gaatctgttg tagtctaggc
     2041 ttcagccgat cgtcactaac tcggccgcct tgggagctgg acttccggtt tcttccggtt
     2101 cttcccgcat gtgcgaaatc ttgatgcgct tcaatactgc ttcgatactc tcgttcacct
     2161 tgcgcagcgg ctcgcgaatg ttg
//
LOCUS       DATMGX010000500         3849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_193006, whole genome shotgun sequence.
ACCESSION   DATMGX010000500 DATMGX010000000
VERSION     DATMGX010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193006"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /gene="lpxC"
                     /locus_tag="VNR65_08095"
     CDS             complement(<1..874)
                     /gene="lpxC"
                     /locus_tag="VNR65_08095"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HWJ18672.1"
                     /translation="MSFRSYSSRQSTVDRDIVLKGTGVHSGAEVSLILHPAEANTGYN
                     FYVQGGGQIGHVPGDFRAVSNLTLCTVLSGMHGVSVATVEHLLAACRGLGVDNVDIEV
                     DGNEIPIMDGSAEPFVDAIEEAGLHELDEPRRYIKVLRPVQVKDGDAIGEIFPYNGFR
                     LDIEIDFNSPFIGKQRLDVDVTPWSFKKKLCRARTFGFMKDVKQLWAAGRALGASLEN
                     TVAIGDDRIINIEGLRYSDEFVRHKAMDAVGDLALAGAPILGLYRSSRGGHRLNSAVL
                     HALYDNKDAWTIVEM"
     gene            complement(1189..2769)
                     /gene="ftsZ"
                     /locus_tag="VNR65_08100"
     CDS             complement(1189..2769)
                     /gene="ftsZ"
                     /locus_tag="VNR65_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWJ18673.1"
                     /translation="MGINLQLPGLTELRPRITVIGVGGAGGNAVNNMIENGLEGVDFI
                     AANTDAQALSTSSAGTVVQMGVGITEGLGAGSRPEIGAAAAEETLDEIRSNLEGVHLL
                     FITAGMGGGTGTGAAPVIARTAKELGVLTVAVVTKPFEFEGQRRMRTADAGIAALAKH
                     VDTLIVIPNQNLFLVASEKTTFADAFMRADEVLKSGVSCITDLMVKEGLINLDFADVR
                     TVMANMGTALMGTGEAEGDKRALQAAEAAISNPLLGEVSMRGAKGLLVSITGSFNMTL
                     YEVEEAASRIRREVDADQNPEVNIIVGATFDPSLQNRIRVSVVATGIAPGEHVPLMPQ
                     AFSERELSLADRISPTAPKRGFLPSRESANAQRPQQDRRVVLEPKGRKGPENEGQGLV
                     VSPESAQRFEPLPPAKTARPPRRPLTFQDFPELSQGNAPANGAPANRASSKPRGFLDW
                     MAGKDRSSKASSSSKSVAIQQRPQGEKPVSEQEYRNGSSLTSGRGQSDGYASDASSGR
                     QNPEEALEIPKFFKKPVS"
     gene            complement(2842..>3849)
                     /locus_tag="VNR65_08105"
     CDS             complement(2842..>3849)
                     /locus_tag="VNR65_08105"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HWJ18674.1"
                     /translation="EAEAGMTVEDVLVTGQFQGLTAETFGATLGEEGTLLKEDIDAIS
                     IAAGEYCTKAQRKLLHLFMTSGDSDDGEPGQVGSVDSGETDVVAISMPLRGARQIASC
                     FAKSLLTVRGYVAGPIASALAVTNTLERSAGILVIDMGAQSTGCALFLRGVPVFADCI
                     TAGGQQISEEIARAFNLRKFEAERLKVRYGSVYDSLQADVDLPIQNGETHEPVSKFSL
                     NHVIRSKTSTLLQAVNDRLKCAGYSIPVGGVVLTGGGSLLPGVRELASHLLASEVRTA
                     KPINLNGLSAGNALSALVGGCLYASRHQSAGEIPYAPELLAQDSSYASRISQWLRTSF
                     L"
BASE COUNT          771 a         1172 c         1115 g          791 t
ORIGIN      
        1 ccatctcgac gatggtccag gcatccttat tgtcgtaaag ggcgtgaagc acggccgaat
       61 tcagacgatg accgccacgc gaggaacggt agagcccaag aatcggagca ccggcgagcg
      121 cgagatcgcc aaccgcgtcc atcgccttgt ggcggacgaa ctcgtcgctg tagcgcagcc
      181 cttcgatgtt gataatgcgg tcgtctccga tcgccaccgt gttctcgagc gacgcgccca
      241 gcgcgcgccc cgcggcccag agctgcttta catccttcat gaaaccgaag gtgcgggcgc
      301 ggcagagctt cttcttgaat gaccaaggcg tgacatcgac gtcgagacgc tgcttgccga
      361 tgaaaggcga gttgaagtcg atttcgatgt cgagacggaa gccattgtaa ggaaaaattt
      421 ctccgatggc gtcgccgtcc ttcacctgca cggggcgcag gaccttgatg tagcggcgcg
      481 gctcgtccaa ctcgtgcaac ccagcctctt cgatagcatc gacgaagggc tctgcactgc
      541 cgtccatgat cggaatttca ttgccatcaa cttcgatatc gacgttgtcg acgccgagac
      601 cgcgacaggc cgccagcaga tgctccacgg tcgccacaga cacgccgtgc atgccgctca
      661 ggaccgtgca aagcgtcaga ttgctgaccg cccggaaatc gcccggtacg tggccaatct
      721 ggccgccgcc ctggacgtag aaattatagc cagtgttagc ttcggcggga tgcaggatca
      781 acgagacctc ggcgccgctg tggacgcccg tgcccttcag gacgatatcg cgatcaacgg
      841 ttgattgcct actcgaatag cttcgaaagc tcataagttt gcgccgttct tttgtgccat
      901 ggcgctttcc ctgcccccaa ggaagacgcc tccccaacgc gagtggatgc tcaaagccta
      961 agccgtaaag catcggtact ctcacgctct tctaggaacc cagctggctc cgcacaccga
     1021 agactcacta catccaacaa tgcgaaccta gctgcggcag cctcggcgct tcaaatcacg
     1081 ctttctaaca atgtgttacg ggggagggtc aaaatggact tatcaacatg agacagctgg
     1141 ccacatggca ggcgcatgcg ggtctgcgag atcgggagaa acgcgattct aagagacagg
     1201 cttcttgaaa aactttggaa tttcaagtgc ttcctcggga ttttgccggc ctgaggaagc
     1261 gtcgctggcg tagccgtcgc tctggcctct tccagaggtc agcgaagagc cgttgcgata
     1321 ctcttgctca gaaaccggtt tttcaccttg tgggcgttgc tgaattgcaa cggatttcga
     1381 ggaagaggaa gccttcgagc tacggtcttt gcctgccatc cagtccaaga atccgcgtgg
     1441 ctttgaggat gctcgattcg ctggtgcgcc atttgcgggc gcgttcccct ggctgagctc
     1501 cgggaagtcc tgaaaagtca atggcctgcg cgggggccgc gcggttttcg cgggcggcag
     1561 aggttcgaat cgctgcgcgc tttctggcga gacgacgagg ccctggcctt cattctccgg
     1621 tcccttcctc cccttcggct ccagcacgac acgccggtcc tgctgcggcc tctgagcgtt
     1681 cgccgactcg cgggacggca ggaagccgcg ttttggcgcg gtcggggata tccggtcggc
     1741 gagactgagt tctcgctcag agaaagcttg cggcatcagg ggaacgtgct cgcctggggc
     1801 gatccccgta gcgacgaccg acacgcgaat gcggttttgc agagaggggt cgaaagtcgc
     1861 gccgacgata atgttcacct ctggattttg gtccgcatcg acttcgcggc gaatacgcga
     1921 cgccgcctcc tcgacctcgt acagcgtcat gttgaaactg ccggtgatgc ttacgagcag
     1981 gcccttcgcg ccgcgcatgg aaacctcgcc caaaagcggg tttgagatcg cggcctcggc
     2041 agcctgaagc gcacgcttgt cgccctcggc ctcgccggtt cccatcaacg ccgttcccat
     2101 attcgccatg acggtgcgga cgtcggcaaa atcgaggttg ataagacctt ccttgaccat
     2161 aaggtcggta atgcacgaga cgccggattt cagcacttcg tccgctcgca tgaaagcgtc
     2221 ggcaaatgtg gttttctcgc tcgcaaccag gaagaggttc tggttcggga ttacgatcag
     2281 cgtatcgaca tgctttgcga gcgccgcgat gccggcatcg gcagtacgca tccgccgctg
     2341 accctcgaac tcaaacggct ttgtcacgac cgcgaccgtc aggacgccaa gttcttttgc
     2401 tgtccgggcg atgacgggcg cggcgcccgt acctgttcca ccacccatgc cggcggtaat
     2461 gaacaggaga tggactcctt ccaggttcga gcgtatttcg tccagcgttt cctcggcggc
     2521 ggcggcgccg atctccggcc tggagcccgc tccgaggcct tcggtgatcc cgacacccat
     2581 ctggacgacg gttcccgcgc tcgacgtcga caaggcctgc gcgtccgtat tcgcggcgat
     2641 gaagtcgacg ccttcaaggc cgttctcgat catattgtta acggcgttgc cgcccgcgcc
     2701 gccgacacca atgactgtga tgcgcggccg caactccgtc aatcctggaa gctgcaggtt
     2761 tattcccatg gcaggcctcg tcggctccgt tcttgagctt tgggctcgct tatgcctgca
     2821 gccgattgcg cggagccgac tttagagaaa gcttgtccgc agccattggc tgattcgaga
     2881 cgcgtagcta ctatcttggg ccagaagctc gggcgcgtat ggtatttctc cagcggactg
     2941 atgccggctt gcataaagac accctccaac aagcgcactc aacgcattcc cggcgctgag
     3001 accgttcaag ttgatgggtt ttgccgtcct gacttccgac gcgagaagat gtgaagcaag
     3061 ctcgcgaact cccggaagca gactgccgcc cccggtcagc acgactcccc ccacgggaat
     3121 agaatatcca gcacacttta accggtcatt aacagcctgg aggagggtcg atgtctttga
     3181 tcgaataaca tgattaagag aaaatttgga cacaggttcg tgggtttcac cattttggat
     3241 cggtaaatct acatcagcct gcagactatc gtagacactg ccgtagcgga ccttcaggcg
     3301 ttctgcttca aatttgcgaa gattaaacgc gcgcgcgatt tcttcggaga tctgctggcc
     3361 gccagccgtg atgcaatcgg cgaagacagg cacgccgcgc aaaaagagag cgcagcctgt
     3421 agattgcgca cccatgtcga tcaccaggat tccggccgat cgctccaatg tgttggttac
     3481 agccagagca gacgcaatag ggcctgcgac gtatccgcga accgtgagaa gactcttggc
     3541 gaagcacgac gcaatttggc gcgcgccgcg aaggggcatg gaaatcgcga cgacatcggt
     3601 ttcgcctgaa tcgacgctgc cgacctgccc cggctctcca tcatcggagt cgcccgaagt
     3661 catgaaaaga tgaagcagct tccgttgcgc cttcgtgcaa tactctccag ccgcaatcga
     3721 aatcgcgtcg atgtcctcct tgaggagcgt gccttcttcg cccagcgttg caccgaaagt
     3781 ctcggcggtc aggccctgga actggcccgt cacgaggacg tcctcgacag tcatgccagc
     3841 ttcggcttc
//
LOCUS       DATMGX010000501         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_102639, whole genome shotgun sequence.
ACCESSION   DATMGX010000501 DATMGX010000000
VERSION     DATMGX010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102639"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VNR65_08110"
     CDS             <1..700
                     /locus_tag="VNR65_08110"
                     /inference="COORDINATES: protein motif:HMM:NF006543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HWJ18675.1"
                     /translation="GTSTETRIVAATKELDDQKGLTAQALAQVELLNQQILALRKQLA
                     ALENSLGDTDKRDKQAQSQIADLGQRLNVALAKRVQELSQYRSSFFGELKRSLGERND
                     VQVVGDRFVFQSEIFFDSGSADLAPQGYAELDKLAAALKDLETRIPKDLNWVLRIDGH
                     TDIRPISTGNFRSNWELSSSRAISVVKFLISKGVSPGRLVAAGFGEYQPLSSGGSDGD
                     LRRNRRIELKLTEK"
     gene            complement(873..1514)
                     /locus_tag="VNR65_08115"
     CDS             complement(873..1514)
                     /locus_tag="VNR65_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18676.1"
                     /translation="MSFITEFDALSPPVSRFDSFFDVYGLSLKQQADLEQRTITLIAR
                     SPASPAAKALATNLARFQPLSVEVNIIFAQIAPADALDTMVRSLGSACRQSPELIIRW
                     AKNRALLDAHERLTLGRTLCWTGDAMRRSEDSRSALDRVEDCTPSILSEANASFAYLW
                     RASKPLPKTLFSPPRTPVSMVEYAAAPLSACQTSMSLEANIVRLEDYLRFRRH"
     gene            complement(2060..>2805)
                     /gene="glmM"
                     /locus_tag="VNR65_08120"
     CDS             complement(2060..>2805)
                     /gene="glmM"
                     /locus_tag="VNR65_08120"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWJ18677.1"
                     /translation="LGVNPDGRNINHECGSTVPETICRKVLEAGAHVGIALDGDADRV
                     VIADENGQLVDGDQILAVIAGSFQDTGRLRGNGIVATVMSNLGLERHLEASGLSLIRT
                     KVGDRYVVEEMRRGDYNVGGEQSGHVVLSDFTTTGDGLVTALQVLSVVVQSGRSVSEV
                     CRRFEPVPQLLKSVRFQGGNPLEDAAVKAAVEAGKARLGKGGRLIIRPSGTEPVIRVM
                     AEGDDKSLVTGVVNDILASIEHTASATAA"
BASE COUNT          604 a          775 c          840 g          586 t
ORIGIN      
        1 cggcacctcg actgaaacgc ggatcgttgc tgcgacgaaa gaactcgatg atcagaaggg
       61 attgacggcg caggcgcttg cgcaggtcga gcttttgaac cagcaaatcc tggctttgcg
      121 gaagcagctt gccgcgctgg agaattcgct cggcgacacc gacaaacgcg acaagcaggc
      181 tcagtcacag atcgccgact tggggcagcg tttgaacgtt gcgctcgcga agcgcgtgca
      241 ggaattgtcg cagtaccgct cctcgttttt cggcgagctg aagaggtcgc taggcgagcg
      301 gaatgatgtc caggttgtgg gcgaccgctt cgttttccaa tcggagattt tcttcgatag
      361 cggttcggcg gacctggctc cacagggcta cgcggaactc gacaagctgg ctgcagcgct
      421 caaggatttg gaaacgcgca tacccaaaga cctgaattgg gttctgcgga tcgacggaca
      481 caccgatatc aggccgattt cgacaggaaa cttcaggtcg aactgggagc tgtcgtcttc
      541 gcgggcgata tcggtcgtga aatttctgat ttcgaagggt gtctcgccgg gaagactcgt
      601 agcggctggc ttcggtgaat atcagccgct ttcgtccggc ggttccgacg gcgatttgcg
      661 ccgcaatcgc cggatcgaac tcaagctcac ggagaagtag gcatcgccgt ccctttgcag
      721 gtacggcgat ggtttctgtg aagagcgttt gacgtgacgc tcgctgttcg ggctcagatc
      781 aacccgcctt taatcggaac cgattaaagg cggaaaagtt gatcgattct aaaatgctag
      841 agcagcccgc gtccaaatgg acgcgggctg ctctagtgac ggcggaagcg caggtagtct
      901 tccagccgga cgatgttagc ttcgagagac atgcttgtct ggcaggcgga gaggggtgcc
      961 gcggcgtact ccaccatgct taccggcgtt cggggcggag agaagagcgt ctttggcaaa
     1021 ggcttcgatg ctcgccaaag gtaggcgaag cttgcgttcg cttccgagag gatcgaaggc
     1081 gtacagtcct caacacggtc caacgcggac cgcgagtctt cggaacgccg catcgcatcg
     1141 ccagtccagc acaatgtcct gccaagcgta agccgctcat gagcatcgag aagggcgcga
     1201 ttttttgccc atcgaataat gagctcgggc gactggcggc aggccgaccc caacgaacgc
     1261 accatagtat ccagggcatc agcgggggcg atctgggcga agatgatgtt cacctcaacg
     1321 gagaggggtt gaaatcgcgc gagattggtt gcgagcgctt tcgcagcggg tgaggcggga
     1381 gaacgggcaa ttaatgtgat ggtcctttgc tctagatcgg cctgctgctt gaggctcaga
     1441 ccataaacat cgaagaagga gtcgaagcgg cttactggtg gggacaaagc gtcaaattct
     1501 gtaatgaagc tcatggcttt ggtccggggc ttgtgcaagg gaggcggggc gcgcgcacct
     1561 cttcttggta aacctccaag gttaaataaa acttgagatt gggccggaac tactcaaaaa
     1621 ttgcgtgaag gaaattcaga catctttaag agtaaagtaa atgttgcgta tttgttaaga
     1681 tattcgtttt cgaggcgcaa gggcatctcg acgacgccct acgccggttc acggtccgag
     1741 aaccttcgaa atctgattgc aggacgctaa ccgctggagc agccagcgtc catctggagg
     1801 cggatcgagc tgctctctaa aactttaaag cgcgtcgaaa ttattcggaa tcgccttatt
     1861 gtcatggccg ccctccgagg cggccatcca cgataacaaa ttccatcttt ttggatcttg
     1921 aggcgggatg gccgggtcaa ccctcgggtc aagcccgagg gcatggcccg gccatgacat
     1981 cggttcacgc taatcgcaac acgcattaga agagatcgac tttccgcctt ttatcgattt
     2041 cgattagagg cgatttgatc tacgcggctg tggcggacgc ggtgtgttca atcgaagcca
     2101 ggatgtcgtt cacaacgccg gtgactaagc tcttgtcgtc gccctcggcc atgaccctga
     2161 tgacgggctc tgtgccggac gggcggatga taagcctccc gcccttgcca aggcgcgctt
     2221 ttccggcttc gaccgcggct ttgaccgcgg catcctcgag cggattgccg ccctggaacc
     2281 gcacgctctt caaaagctgt ggcacgggct cgaatcggcg gcatacttcg ctaaccgacc
     2341 ttccgctctg aacgacgaca ctcagtacct gaagcgccgt cacgagcccg tctccggtgg
     2401 tcgtaaagtc cgaaagcacg acgtgacccg actgctcgcc gccaacatta taatcgccgc
     2461 gccgcatttc ctcgaccaca tagcggtcgc caaccttggt ccggatcaat gaaaggccgc
     2521 tcgcttcgag atggcgttcc agaccaagat tggacatcac ggttgcgaca ataccgtttc
     2581 cacgcaagcg gccggtatcc tggaaactgc cggcgatcac cgcaaggatc tggtcgccat
     2641 cgaccagctg cccgttctcg tcggcaatca cgacacgatc cgcgtctccg tcgagcgcga
     2701 tgccgacatg cgctcctgcc tcaaggactt tccggcaaat cgtttcgggc actgtagagc
     2761 cacactcgtg attgatattg cggccgtctg ggttgacacc gaggg
//
LOCUS       DATMGX010000502         2271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_56787, whole genome shotgun sequence.
ACCESSION   DATMGX010000502 DATMGX010000000
VERSION     DATMGX010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56787"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1304)
                     /locus_tag="VNR65_08125"
     CDS             complement(150..1304)
                     /locus_tag="VNR65_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ18678.1"
                     /translation="MKKYFAILLFALLALGLCVPPVFAQASGSVKGVCKDAQGNPVAD
                     GVIVWKNMDNGQTYKLTTNKKGEYFSLGLSPGTYLVTLYKNADDFKANKELDHVKGFK
                     VNLDENELPFDLKKDQEAAAKGQGLSPEQLKQMQEAQAKQAKETNTVKGLNEKLNAAK
                     TAADAGDYDTAIAALTEATQMDATRDLIWFKLGDYYRMSAPKQTDPAEKQKRLDSAVE
                     AYQKAVDLKKAATNDKDPNATKNLAAYYNNLADAYYKAKKIDDAVKTYEIAAQTDPSA
                     VAQAYFNIGAVLTNSGRPDEANAAFDKCLAADPTRAEAYYQKGLNLLGKATLQGDKTV
                     APPGTAEAFQKYLELAPTGPNAESAKALLASIGSTVETSFGTKKKTPPKK"
     gene            complement(1377..2057)
                     /gene="queC"
                     /locus_tag="VNR65_08130"
     CDS             complement(1377..2057)
                     /gene="queC"
                     /locus_tag="VNR65_08130"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HWJ18679.1"
                     /translation="MTISKRSRAVVLLSGGMDSCVCAALAARDHETAAVHISYGQRTE
                     ERERQSFLAICQRLKIHDKLMVRNEALRAIGGSALTDAAIDVPTAEEIGQTVPVTYVP
                     FRNAHFLAVAVSWAEVLGAKKVYIGAVEPDSSGYPDCRPAYYKAFNEVTKAGTKEGLI
                     EIVTPLIAMRKTDIVRLGLELDAPFDLTWSCYSREDQACGICDSCVLRLRAFAAAGMQ
                     DRISYAAR"
BASE COUNT          448 a          641 c          642 g          540 t
ORIGIN      
        1 tgggaagaaa actcagatag ttaactgtac cgcgacagag gccgtgtgcg aagaacagca
       61 agtccataga cctaagtaac ctcctggggc cagacgcaca aagggcggct cggttgagcc
      121 gcccttccgc ctgactggat cggtagtccc tacttcttag gaggggtctt cttcttcgtg
      181 ccgaagctgg tttccactgt ggatccgatg ctggcgagca aggccttggc gctctcggca
      241 ttcgggcccg tcggtgcaag ttccaggtac ttctggaagg cctcggctgt ccccggaggg
      301 gcgacggtct tatcgccttg gagggtagcc ttgcccagca ggttcagtcc cttttgatag
      361 taggcttcgg cccgggtggg gtcggccgca agacacttgt cgaaggccgc attggcctcg
      421 tctggcctgc cggagttggt gaggacggcg ccgatgttga agtaagcctg agcgaccgcg
      481 gatggatccg tctgagccgc aatttcgtag gtcttcaccg catcgtcgat tttctttgcc
      541 ttgtagtaag cgtccgccag gttgttgtag taggcggcaa ggttcttggt tgcgttggga
      601 tccttatcgt tggtggcggc ctttttgagg tccacggcct tctgataagc ctccacggct
      661 gaatcaaggc gtttctgttt ttcagccgga tcggtctgct tgggcgccga catacggtaa
      721 tagtcgccca gtttgaacca gatcagatcg cgggttgcgt ccatctgggt ggcctcggtc
      781 agggccgcga tggccgtgtc gtaatcgccg gcatccgcgg cggtcttggc cgcgttgagc
      841 ttctcgttca gacccttgac ggtattggtt tccttcgcct gcttggcctg ggcctcctgc
      901 atctgcttca actgctcggg tgaaagtccc tggcctttgg cggcggcttc ctggtccttc
      961 tttaggtcaa agggcagttc gttctcgtcg agattgacct tgaagccttt gacgtggtcc
     1021 agttccttat tcgccttaaa atcgtcggca ttcttgtaga gagtgaccaa gtacgtgccc
     1081 ggcgagagcc ctagcgaaaa atattcgccc ttcttattag tcgtaagctt gtaggtctgg
     1141 ccgttgtcca tgttcttcca gacaatcacc ccgtcggcga cgggatttcc ctgggcatcc
     1201 ttacaaacgc ccttgaccga tcctgaagcc tgggcgaaga ctggaggaac gcacagtccc
     1261 agcgccagta aagcgaatag aagaattgca aaatattttt tcatcttgcc tcccggcaaa
     1321 gttaaatttt gctcgccgat ctccaaaagc cgatcgaaat ctgttcccac aaatatctat
     1381 cgcgccgcgt acgaaattcg atcctgcata cccgccgccg caaaggcgcg caggcgcaac
     1441 acgcagctat cacagatccc gcacgcctga tcctcgcggc tgtaacatga ccaagttaaa
     1501 tcgaagggag catcgagttc aaggccaagc cgcactatat cggttttgcg catggcgatg
     1561 agcggggtga cgatctcgat gagaccttcc ttcgtgcctg cttttgtcac ttcattgaac
     1621 gccttgtagt acgccggccg gcaatccgga tagcccgaac tgtccggctc gactgcgcca
     1681 atgtacacct ttttcgcgcc caacacctcg gcccaactca cggcgacagc caggaaatga
     1741 gcattgcgaa aaggaacgta ggtgacggga accgtctgtc cgatctcttc ggcggttggc
     1801 acatcgatgg cagcgtcggt gagcgccgac ccgccaatgg cgcggagagc ctcgtttcgg
     1861 accatgagct tgtcgtgaat tttcaagcgc tggcagatgg caagaaacga ctggcgttcg
     1921 cgctcctcgg tccgctggcc atagctgatg tggacggcgg cggtctcgtg gtcgcgtgcc
     1981 gccaaagcgg cgcaaacgca ggaatccatt ccaccgctca agagcacgac cgcacgggat
     2041 cgctttgaga tcgtcatcaa taaagattgt tgattgctga ttcttgattg ctgattgaaa
     2101 acccaaagtg gctcatcagg tgttcaatca aaaatcaaca atcagaaatc atcaatcgcg
     2161 tctctatact cctttcactg ccgggtccca tataaatttg tgcaactgca gtcccagcct
     2221 cgcagggacg ttatcggcga gcatccactc cgccagttcc tgggggtcga g
//
LOCUS       DATMGX010000503         7025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_412491, whole genome shotgun sequence.
ACCESSION   DATMGX010000503 DATMGX010000000
VERSION     DATMGX010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412491"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..905)
                     /locus_tag="VNR65_08135"
     CDS             complement(63..905)
                     /locus_tag="VNR65_08135"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HWJ18680.1"
                     /translation="MRYIEHLSRMAVWSYRFAILAVLIFAGAFVWHRFFGLPTPFALK
                     VLGGAVAGAVVSLVVAVAALVTIWKEGHLGAGRASAAVFLSALVLAVPLWSLPGLLRL
                     PRIYDVTTDTGSPPAFDRVAKIRQGQANPAHYDPSFSTLQAAAYPDIKPLQIQRSLID
                     VYSAARDVVKALHWKIIDEQAPEATRSGHIEAMDRTLFFGFTDDIAIRVTGSAKAAKV
                     DVRSSSRFGQHDLGRNAERIRRYLSEVKFRLAELDRQDRMERVMATHEKDKAKPAPAK
                     SNRK"
     gene            1085..1372
                     /locus_tag="VNR65_08140"
     CDS             1085..1372
                     /locus_tag="VNR65_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18681.1"
                     /translation="MSEDHQWRQLGSIVNQVLMNTRTKAVRTGTQSKAPPRPIAGKTQ
                     QRPAGLVAGKLGHGFLNPEYKAPPPAQLELPFGIEAEANFDRRPAKGARLM"
     gene            complement(1386..2669)
                     /locus_tag="VNR65_08145"
     CDS             complement(1386..2669)
                     /locus_tag="VNR65_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWJ18682.1"
                     /translation="MRVVLRFAVVTPLVICLTGCLNFFGTGSDDVVTPGDRQLPASTQ
                     AMLAIKGMKLEAPIFVRIFKEESEFEVWKYKDGRFQLFRTYPICAWSGELGPKVQQGD
                     RQAPEGFYTVSRAQMNPHSLYHLAFNIGFPNTHDQVNGYSGSALMVHGNCKSAGCYAM
                     TDAYIEEIYILAREAFNAGQTKFHVQALPFRMTTLKMILHRGNRWYPFWSRLKEGYDA
                     FEDTGKPPIVKVCGKQYLVNVSFPGGEPAPDAACPIYAKINPSQMPGIDGVPQTVLAS
                     LNKGEAPSRQPAQAPAPVVMAAAAAPAAPYKPQPPVVEMADMSASPVITRPAAPSQMA
                     SLQSMKAPASPVYPAAPKTTASAPLPAPVRVAASASPVQPVTYRFGDTVASPSAAPAQ
                     EASKAVDSDAIQKPNRSGKGGKLSNTSGEPTDTQH"
     gene            complement(2855..3874)
                     /locus_tag="VNR65_08150"
     CDS             complement(2855..3874)
                     /locus_tag="VNR65_08150"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HWJ18683.1"
                     /translation="MLGLHRYPNLGFSGSPFPMRTYLDFEKPIAELESKIEELSALGD
                     GDSAVSIIEDIARLREKAQQALVETYSSLDPWQKTQVARHPQRPHTCDYIRELIVDFT
                     PLGGDRYFGEDLAIIGGLGQFHGRTVMILGHEKGADTQGRLRHNFGMAKPEGYRKAVR
                     LMQMAERFGLPVVSLVDTAGAYPGIEAEERGQAEAIARSTDCCLSLTVPMIAIIIGEG
                     GSGGAVAVASANRVLMMEHSIYSVISPEGAASILWRDSAKARDAATNMKITAQDLLQL
                     GIIDGIIPEPTGGAHRDPDVAIKEVSDAIELALKDFENLDGVDIRNQRHEKFLTIGRS
                     LPSGR"
     gene            complement(3954..4649)
                     /locus_tag="VNR65_08155"
     CDS             complement(3954..4649)
                     /locus_tag="VNR65_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ18684.1"
                     /translation="MTGTYNQFCPVAMAAEILGTRWTLLLLRELVAGSTRFNELRRGL
                     PRMSPALLSKRLKDLEAAGIVTRRRLEADPELFEYRLTDAGNELGQVIEAVGAWGHRW
                     IETEASLKNLDPTLLMWDMRRNIGPFPTPRCRNTIQVIFTDLKEQQRNWWLIVEPNQE
                     ADLCSVNPGFDVDLYLSTDLRTMTEIWMGYTTIDRAKEQGKLLLTGNRQLEADLGTWL
                     RLSRFAKVERMVA"
     gene            4831..5058
                     /locus_tag="VNR65_08160"
     CDS             4831..5058
                     /locus_tag="VNR65_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HWJ18685.1"
                     /translation="MPIVDIHLIEGVFDKNQKQEMIRKVTDAMVDIEGETMRGVTWVR
                     LYEVASGEWGMGGKGITTADVKAMQTRSTRL"
     gene            complement(5237..>7025)
                     /locus_tag="VNR65_08165"
     CDS             complement(5237..>7025)
                     /locus_tag="VNR65_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWJ18686.1"
                     /translation="VQTLQKFTKAELDMARAHNEHGLISETMRASEFPGAYGDMAQNL
                     NAMVKGHIDVQTRFTELMGEYASGRFETHMPPLPGERKAISDAAEKVRAGLEASAKAA
                     HYNARVKAALDHVSVPVRIADSDGVINYVNHAMNGMLHKYAPAFRRQNPSFDPDKVVG
                     GEVGMLYADPAAAVANLRRLTQSAVFRTDVGDRNYDLIITPVSDEKGARLGTVGQWTD
                     ITEQLGAEREIGAIVEAAAAGDFSKRIADADKSGFMLQMAQGVNAILGTSEQALGEIG
                     RILKALAQGDLSQEIRADFKGVFADLKDNSNETIANLRGIITRIREASESINTAAREI
                     ATGNNDLSRRTEEQASSLEETASSMEEFASTVRQNAENAQEANKLAAEASESAQQGGE
                     VVARVVTTMASITESNREIADITTLIDGIAFQTNLLALNAAVEAARAGEQGRGFAVVA
                     SEVRNLAQRAAEAAKSIKAVIANSIGKVEDGARLVESAGQSMEKMVAQVKRVSAIIGE
                     IASASKEQSGGIEQVNQAITQIDQITQQNAALVEEATAAAKSLEDQSDALVQGVAVFK
                     LVHGQGAAQKREPVAQRADALADGLAVLH"
BASE COUNT         1340 a         2057 c         2123 g         1505 t
ORIGIN      
        1 actggagaag ctagacgata tgacccgaac gctctgacgg ttgattgacg agccggaacg
       61 ccttatttgc ggttgctttt tgcaggggcc ggtttcgcct tgtccttttc atgcgtcgcc
      121 atcacgcgct ccatgcgatc ctggcggtcc aactctgcca agcggaactt aacctcggac
      181 aagtaccggc gaatgcgttc cgcatttcgt ccgagatcgt gctggccgaa gcgcgacgac
      241 gaccggacat cgacctttgc cgctttcgcc gacccggtaa cgcggatcgc aatatcgtcc
      301 gtgaacccga agaagagggt gcgatccatg gcttcgatgt gcccgctcct ggtcgcttcg
      361 ggcgcttgct cgtcgatgat cttccagtgg agcgctttga ccacgtcgcg cgctgcggaa
      421 tagacatcga tgagggatcg ctggatttgg agcggtttga tgtcgggata ggccgccgcc
      481 tgaagcgtgc tgaacgaagg atcgtagtgc gcggggttcg cctgaccttg gcggattttt
      541 gcgacgcggt cgaaagcggg aggcgagccg gtgtccgttg taacgtcgta gatccttggc
      601 aatctcagga gcccaggaag cgaccacaat gggacggcga gtacaagtgc gctgagaaac
      661 acggcggcgg atgcacggcc cgcaccaaga tgcccttcct tccagatcgt cacaagcgcc
      721 gcgaccgcca caaccaggct gacgacggcg cccgcgaccg ctccgcccaa taccttgagc
      781 gcgaagggtg tcggaagccc aaaaaagcgg tgccagacaa acgccccagc gaatatgaga
      841 accgccagga tggcgaacct gtagctccag acggccattc gggagagatg ttcgatgtag
      901 cgcaaagggt cttgctctcc agatccgagt ttataagcta agactcacgg cagccttgca
      961 cttttccaaa atataatcaa aaattcgctt gcagtgtcgc aatggagcca ccgaatcact
     1021 cgacgctgac ctcgacgccg accttaaggg ccaagcgtcg tgtagtaaat gatggagtac
     1081 gaccgtgagt gaagatcatc aatggcggca actcggttcg atcgtcaatc aggtgctcat
     1141 gaacactcga acaaaagcgg ttcgcacggg aacgcagtcg aaagctcccc ctcgcccaat
     1201 cgccggcaaa actcaacaaa ggccggctgg tttggtggct gggaagctgg ggcatggttt
     1261 tctgaaccca gagtataaag cacccccccc agcgcagctt gagcttccct ttggaatcga
     1321 ggcggaggcc aacttcgaca ggcgcccggc aaaaggcgcg cgcctcatgt gaggcgcgca
     1381 aagcgttagt gctgcgtatc ggtaggctcg ccggaggtgt tgctcagctt gccacctttt
     1441 ccggagcggt tcggcttttg gatcgcgtca gaatcgacgg ccttcgatgc ctcttgagca
     1501 ggtgcggccg aaggcgaggc tacggtatca ccaaaccgat aagtgacggg ctgaaccggg
     1561 ctcgcgctcg cggcaacacg aaccggcgcg ggcaatggtg ctgaagccgt tgttttcggc
     1621 gcggccggat agacgggcga ggcgggcgct ttcatggact gaagacttgc catctggctc
     1681 ggcgcggctg gacgcgtgat gaccggcgac gcggacatgt ccgccatttc aacaacaggt
     1741 ggctggggtt tgtacggggc cgctggcgcc gccgccgctg ccatgacgac aggcgcggga
     1801 gcctgtgccg gctgccgcga aggtgcttcg cccttgttca aactggcaag gacggtctgc
     1861 ggaacgccat cgatgcctgg catctgtgag ggattgattt tcgcatatat cgggcacgca
     1921 gcgtcgggcg ccggctcgcc accagggaaa gatacattga cgagatactg cttgccgcac
     1981 accttcacga tcggcggttt cccagtgtct tcgaaggcgt catatccttc tttcaaccgg
     2041 ctccagaagg gataccagcg attgccgcgg tgcaggatca ttttcaaggt ggtcatgcgg
     2101 aacggcaaag cctgaacgtg aaacttcgtc tgccccgcgt tgaacgcttc gcgggcgaga
     2161 atgtagatct cttcgatata ggcgtcggtc atggcgtagc agcccgcgga cttgcagttg
     2221 ccgtgaacca tgagcgccga gccagagtag ccgttcacct gatcgtgggt gttcgggaag
     2281 ccgatgttga acgccagatg gtaaaggctg tgcgggttca tctgagcgcg gcttacggta
     2341 tagaagccct ccggggcctg cctgtcgccc tgctgcactt tcgggccaag ctcgcctgac
     2401 cacgcgcaga tgggataagt gcggaagagc tggaaccgcc catccttgta cttccacacc
     2461 tcaaactcgg attcttcttt gaaaatgcgg acgaagatcg gtgcttccag cttcatgccc
     2521 ttgatggcaa gcatcgcctg cgtggaagcc ggcaactgtc tgtcgcccgg ggtaacgacg
     2581 tcatcggagc ccgtaccgaa gaaattcaaa catccggtca ggcaaattac gagcggcgta
     2641 accacagcaa aacgcaacac aactctcatc accctgtacc cctgcgctac ttcaggagag
     2701 gatggatttt ttggattaag aatccgtttc aatccgaggg taaatcttag atgtcgcatc
     2761 ctgaggttgt ggactcctga ttcgcgtgcc aagtgtccgc aatttcctaa ttttttgggg
     2821 ccaaaatgcg acaaaaggtg cgagagagac aggtctaacg tcctgaagga agggatcttc
     2881 cgatggtcag gaacttttca tgacgctggt tgcgaatatc aacgccatcc aggttttcga
     2941 agtctttgag agcaagttcg atcgcgtcgc taacttcttt gatcgcgaca tcggggtcgc
     3001 ggtgggcacc tccggtcggc tccgggatga taccgtcgat gatgccgagc tggagcaggt
     3061 cctgcgcggt gattttcata ttggtcgcgg catcccgggc cttcgcacta tcccgccaga
     3121 ggatcgatgc tgcgccttcc ggtgagatga cgctgtagat tgagtgctcc atcatgagga
     3181 cgcgatttgc ggaggcaacc gcgaccgcgc cgccggaacc gccctcgcca atgatgatcg
     3241 caatcattgg caccgtaagg gacaggcagc aatccgtgga gcgggcgatg gcctcggcct
     3301 gtccgcgttc ctccgcttcg atgccgggat aagcgccggc ggtatcgacg aggctcacca
     3361 ccggcaggcc gaaccgttcg gccatttgca tcaacctcac ggctttgcga tagccttcgg
     3421 gtttcgccat cccgaaattg tgccgcagcc gcccttgggt gtccgcgccc ttttcgtggc
     3481 cgaggatcat aacggtgcgg ccgtgaaatt ggccaagacc gccgatgatg gcgaggtctt
     3541 cgccgaaata gcggtcgccg ccgagcgggg tgaaatcgac gatcagttcc cggatgtaat
     3601 cacaggtgtg gggcctttgc gggtgccgcg ccacttgcgt cttctgccag ggatcaagac
     3661 ttgaataggt ttctacgaga gcctgctgcg ccttctcccg gagccttgcg atatcctcta
     3721 tgatcgagac cgcgctgtcg ccatcgccaa gcgcgctcag ctcctcgatc ttgctttcga
     3781 gttctgcaat aggtttttca aagtcgagat aggtgcgcat ggggaaaggg gagccggaaa
     3841 agccaaggtt cggatagcga tgtaggccta gcatggcgca aggtccatag ctagccccgc
     3901 ggccctcagt cgctccaatg cggaataagc aattgtgcag caaacggcgc ccgtcaggcg
     3961 accattctct cgaccttcgc gaatcggctc aatcgcagcc aggtccccaa atcggcttcg
     4021 agctgtctgt ttcccgtgag caggagcttt ccctgttcct tcgctcgatc gatcgtggtg
     4081 tatcccatcc agatttcggt catcgtccta aggtccgtcg agaggtacag atcgacatcg
     4141 aaaccaggat tgaccgagca caagtcggct tcctgattag gctcgacgat cagccaccag
     4201 ttgcgttgct gctccttgag atcggtgaaa atgacctgaa ttgtgttccg gcaccggggc
     4261 gttggaaagg gaccgatgtt tcgccgcata tcccacatga ggagggtggg atccaagttt
     4321 ttgagcgacg cttcggtttc aatccatctg tgtccccacg caccgacagc ctcgatgacc
     4381 tgccccaatt catttccagc gtcggtcagc cgatactcaa agagttcggg atcggcctcc
     4441 agccggcgcc gcgtcacgat tcccgctgcc tccagatctt tcaatcgctt cgatagaagg
     4501 gcaggcgaca ttctcggtaa accccgtctc aactcgttga agcgggtaga tcccgccaca
     4561 agttcgcgca aaagcaacag tgtccagcgg gtcccaagaa tttcggctgc cattgcgaca
     4621 gggcagaact gattgtaggt gccggtcatg gttagctcct tacatacgcc cgcctccaac
     4681 ggcttagcgt gagataggtt ccgggagcga gttcactttc tataccgggg gaggttcagt
     4741 tcctgaacta tctctctggc aagcccgcgt gacatggtcc tgtccatgag acggggatga
     4801 gccccgcttg aataaaagag aggaaatatc atgccaattg ttgatatcca ccttatcgaa
     4861 ggcgtcttcg acaagaacca gaagcaggag atgatcagga aggttaccga tgccatggtc
     4921 gacatcgaag gcgagaccat gcgcggtgtg acctgggtcc gcctgtatga ggtagcaagc
     4981 ggtgaatggg gcatgggcgg caaagggatt acaaccgccg atgtgaaggc gatgcagact
     5041 agatcaactc gtctttagcg gaatcgatta aaggcagaaa agctgatcgt gtccataggg
     5101 ttagagaacc cgcgcttgat aacggtgcgt catcgcagtc tttgccggcg ggcgatgacg
     5161 cgtccaggaa gcgccgcctt tgcccctccc gctctccacg ggaacgcgag aagggctctt
     5221 gggcaccctc ggcgcttcag tgcaatacgg caagtccatc tgccagggcg tccgcccgct
     5281 gcgcgacggg ttcgcgcttt tgcgcggcgc cttgcccgtg gacgagtttg aaaacggcaa
     5341 caccctgcac gagcgcgtcc gattggtcct cgaggctctt ggccgccgcg gtggcttcct
     5401 cgacaagcgc ggcgttctgc tgggtgatct gatcgatctg ggtgatggct tggttcacct
     5461 gctcgatgcc gccgctttgc tctttgctgg cggacgcgat ctcgccaatg atggcgctca
     5521 cgcgcttcac ttgggcgacc atcttttcca tggactggcc tgcgctctca acgagcctgg
     5581 cgccgtcctc gaccttgccg atcgagttcg cgattaccgc cttgatgctc ttggctgctt
     5641 ccgctgcgcg ttgtgcgagg ttccgaacct ccgaagccac cacggcgaag ccgcggccct
     5701 gttcgcccgc gcgcgcggcc tcgacagcgg cgttgagcgc gagaaggttc gtctggaagg
     5761 cgatgccgtc gatcagcgtt gtgatgtcgg cgatctcgcg gttgctttcg gtgatgctgg
     5821 ccatggttgt gacaacccgc gccacgactt cgccgccttg ctgcgcgctc tccgacgcct
     5881 ctgccgcaag cttgttggcc tcctgtgcgt tttccgcgtt ctgcctgacc gtggacgcaa
     5941 actcctccat gctgcttgcg gtttcctcaa ggctcgacgc ttgctcctcg gtgcgcctcg
     6001 aaaggtcgtt gttgccggtc gcgatttcgc gcgccgccgt gttgatggac tcggacgcct
     6061 cgcggattcg ggtgatgatg ccgcgcaggt tggcgatcgt ctcgttcgag ttgtctttca
     6121 ggtctgcgaa gacgcctttg aagtcggccc tgatttcctg gctgaggtcg ccctgcgcca
     6181 gcgccttcag aatgcgcccg atctcgccga gcgcctgttc gctcgtgcct aggatcgcgt
     6241 ttaccccttg cgccatttgc agcatgaagc cggacttgtc cgcgtcggcg atgcgttttg
     6301 aaaagtcgcc ggccgccgca gcctccacaa tagcgccgat ctctctttcc gctccgagct
     6361 gctcggtgat gtccgtccac tgtcccacgg tgccaagccg cgcacccttt tcatccgaaa
     6421 caggcgttat aatcagatcg tagttgcggt ctcctacatc cgtgcggaaa accgccgatt
     6481 gagtcagtct gcggaggttg gcgacggcgg cggcgggatc ggcgtagagc atgccgactt
     6541 cgccgccgac caccttgtca gggtcgaagc ttgggttctg ccggcggaag gccggggcgt
     6601 atttatgcag cataccgttc atggcatggt tcacgtaatt gatgacgcca tcgctgtccg
     6661 cgatgcgcac cgggacactg acgtgatcga gcgcagcctt gacgcgcgca ttgtaatgtg
     6721 cggccttggc gctcgcctcg agcccggcgc gaaccttttc tgcggcgtcg gagatcgcct
     6781 ttcgctcgcc cggcagcggc ggcatgtggg tttcgaatct gccactggcg tattcgccca
     6841 tcagctccgt aaagcgggtc tgaacgtcaa tatggccctt caccatcgcg ttcaggttct
     6901 gcgccatgtc gccataggcg ccggggaact cgcttgcacg catcgtctcg ctgatgaggc
     6961 catgctcgtt gtgggcgcga gccatgtcga gttcggcctt ggtgaatttc tgcagggttt
     7021 gtacg
//
LOCUS       DATMGX010000504         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_147198, whole genome shotgun sequence.
ACCESSION   DATMGX010000504 DATMGX010000000
VERSION     DATMGX010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147198"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..195)
                     /locus_tag="VNR65_08170"
                     /pseudo
     CDS             complement(1..195)
                     /locus_tag="VNR65_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            329..535
                     /locus_tag="VNR65_08175"
     CDS             329..535
                     /locus_tag="VNR65_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HWJ18687.1"
                     /translation="MFDPVPKLSDETLIETVRFPTILRNALMTAGFKTIGEIRAKPDD
                     ELGRITRIGKESLSYLRRTLGRAR"
     gene            596..907
                     /locus_tag="VNR65_08180"
     CDS             596..907
                     /locus_tag="VNR65_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18688.1"
                     /translation="MVYATKEWKVSKPLPGAEWQLDARFNAAEALTRDPDLMDVITAA
                     LEKGAEVVHSSEKVKQVEVEVEEMSERTKVQATPELPVIHRSIMSNCRPEFVTLWRTA
                     A"
     gene            1235..1849
                     /locus_tag="VNR65_08185"
     CDS             1235..1849
                     /locus_tag="VNR65_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18689.1"
                     /translation="MAKLTKLDVARHQLGTALDLFIRDRDPIAVQCLACGGGELIDKI
                     ASFADIPTFSTHMLETVPELDIGKLRNLQRQYWNAFKHLEDRGGKPRDDDDILAAFDD
                     TKNDAALFVGWWDYYAVTGKLPLPVQVFQIWWYALNEEKLALGADLDTIRKTFPGISS
                     CDRTERKRRLRRAVEKYRDNAATLKDPRTEVNPLCFPSSVFVGG"
BASE COUNT          471 a          556 c          578 g          428 t
ORIGIN      
        1 cgtatcagcc atgacgacct cggcggatag tccctcgatc aatgtggcgg cttgcggtgc
       61 atcgcccttc tggcctgcgg tcagcgtgaa ccgcacaggg catcccaagc cgcggacggc
      121 catatgtatc ttggtgctca ggccaccacg ggagcggcca atcgcctgat cttcagaccc
      181 cctttttagc cccgcgtcgg atcaacttag gggctcggca cccgagacag aaactgaatc
      241 cttgccagag cgagcatcag gagtgattcc gcctggccaa aaacagtaga gttcgggcta
      301 gcaggcgaag agaaaatagg cggcggccat gttcgacccg gttcccaaat tgtccgacga
      361 aacgctgatc gagacagtcc gctttccgac catccttcgg aacgctttga tgaccgcggg
      421 attcaagacg atcggcgaaa ttcgcgcgaa gccggatgac gaacttggaa ggatcacgcg
      481 gattggcaaa gaatcgttga gctacctccg taggacgctg ggtcgggctc gataaccaat
      541 gccagggctc aagtgttacg tggaatggat gagccgaggc cgacggacag gccgagtggt
      601 ttatgccacc aaagaatgga aagtctcaaa accacttccc ggcgcggaat ggcagctcga
      661 cgcgaggttc aatgcggcag aagcactcac gcgcgatccc gacctgatgg atgtgataac
      721 ggcggctctc gaaaaagggg ctgaggtcgt gcattcatca gaaaaggtga agcaagtcga
      781 agttgaagtt gaagaaatga gcgaacggac caaggtccaa gcgacgcccg aactccctgt
      841 gatacaccgc tcgataatgt cgaactgccg acccgaattt gtaacgcttt ggcggacggc
      901 ggcctaaaaa ccgtcggcga ggttcgcgaa gcgtctgacc aaatgttggt aagcttgcca
      961 aatctcgggg cgcgctcggt tgctcacctt cgaaaggccc tcggtctgta gctcttaagc
     1021 caagctatcc gcaggatatc tacatcctag tcatcggcta ggcaaagtat ggcgcgaaaa
     1081 ctgcatataa aatccggtct gagagggcgt ggctatccag tctcctccct ggacgtttcc
     1141 ttcctagact gaccgtcgca cctcctggta gcggcggttt tttcttcgcc gtggatatat
     1201 gatggtcaga gacgacatga aataatggcg cctcatggct aagctcacga aactggacgt
     1261 tgcgcgacat caactcggca ccgcgctcga cctcttcatc cgagaccgtg acccgatcgc
     1321 tgttcagtgt ctagcgtgcg gaggcggaga gctaatcgat aagatcgcct ctttcgcgga
     1381 tattccgacg ttctccaccc acatgcttga aactgtgccg gagctagata tcggaaaatt
     1441 gcgtaattta cagcgccagt actggaacgc tttcaagcat ctggaagata gaggcggaaa
     1501 gccgcgtgac gatgatgaca tcctggctgc tttcgatgac acaaagaatg atgctgcgct
     1561 gtttgtcggc tggtgggact actatgcggt gaccggaaaa cttccgctgc ctgtccaggt
     1621 ttttcagatt tggtggtacg ctctaaatga agagaagcta gccttggggg ccgatctgga
     1681 cactattcgg aaaacattcc ccgggatttc ctcgtgcgat cgcaccgaga ggaagcggag
     1741 acttagacgc gcggtggaaa aatatcgaga caatgccgcg acactcaaag atcctcgaac
     1801 cgaggtgaac cctctctgct tcccgtccag cgttttcgtc ggcggctgag tagacttttc
     1861 cgtctaaccc gatcatcctc gcggtggacg ggccttgccc ggaacgaaac tgcgtcatag
     1921 cttatccgct ttggtggtcc gtgagttcct gtaatgcgta gatccccatc gatcattccg
     1981 ggtggcgagc ccactgtcta tctgatcgtc gacaatctcg gcaggctcgg caa
//
LOCUS       DATMGX010000505         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_353602, whole genome shotgun sequence.
ACCESSION   DATMGX010000505 DATMGX010000000
VERSION     DATMGX010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353602"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VNR65_08190"
     CDS             complement(<1..711)
                     /locus_tag="VNR65_08190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWJ18690.1"
                     /translation="MIAAPESLQSVFDRAFAGISQAREAFTPRLRSREVGTITSIATG
                     IAKVSGLPGVGFEELVKFPGDVLGIAFNVDEAEIGVVLLGEYWHLHAGDEVERTGRVM
                     DVAVGDGLLGRVIDPLGRPLDGNGPLATSERLPIERPAAPIMDRAPVTVPLQTGLKVI
                     DALIPIGRGQRELILGDRQTGKTAIAIDTILNQRGQNVVCVYCAIGQRASAVAKTVAV
                     LQEKGAMDHTIVVVTEGND"
     gene            complement(708..1745)
                     /locus_tag="VNR65_08195"
     CDS             complement(708..1745)
                     /locus_tag="VNR65_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18691.1"
                     /translation="MLIDWFTIGAQALNFLILVWLMKRFLYKPILNAIDAREKRIAKE
                     LADADAKKAEAQKERDEFQKKNEEFDQQRAALMSAATDEAKAERQRLIDEARKAADAL
                     SAKRQETLRNDAHNLNQAISRRAQQEVFAIARKALTDLATTSLEERMGEVFTRRLREM
                     DGKAKEGLAEALKTASGPALVRSAFDLPVEQRAAIQNALNETFSAEIRVRFETAPNLV
                     SGIELTTNGRKVAWSIADYLASLEKGVGELLKEKDKPEAKAEPKPETKAEPKPEAKAE
                     SKPAAKAEPKPEAKAEPKPETKTEPKPEAKAEPKPEAKAEPKPEAKAGPKPAAKAEPK
                     PEEPKPETKSQ"
     gene            complement(1750..2022)
                     /locus_tag="VNR65_08200"
     CDS             complement(1750..2022)
                     /locus_tag="VNR65_08200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HWJ18692.1"
                     /translation="MTLIAVTSIVTAGLTTSFGCMMPALGEGRSVATALTALAQQPDA
                     SATITRTLFVGLAMIESTAIYCFVVSMILIFANPFWNHVIAQTTGK"
BASE COUNT          326 a          613 c          646 g          499 t
ORIGIN      
        1 gtcgttgcct tcggtcacga caacgatggt gtgatccatc gcgcccttct cttgcaagac
       61 ggccacggtc ttcgccacgg cggaagcgcg ctggccaatg gcgcaataga cgcacacgac
      121 attctggccc cgttgattga gaatggtgtc gattgcaatt gcggttttgc ccgtctgccg
      181 gtcccccaga atcaactcgc gctggccgcg cccgatagga atgagcgcat cgatgacttt
      241 gaggccagtc tggagcggca cggtgacggg cgcgcggtcc atgatgggcg cggcggggcg
      301 ttcgatgggc aggcgttcgc tcgtcgccag cggcccgttg ccatcgagtg gccgaccgag
      361 agggtcgatg acgcgtccca gcaatccatc gcccacggcc acatccatga ctcggcccgt
      421 gcgctcgact tcatcgccgg cgtgcagatg ccagtattcg ccgagcagca cgacgccaat
      481 ttcagcctcg tccacgttga acgcaatgcc gagcacatcg ccgggaaact ttactaactc
      541 ctcaaagccc acgccgggaa gaccggagac cttggcgatg ccggtagcga tgctggtgat
      601 cgtgcccact tcccgtgacc tcagacgcgg cgtgaatgct tcccgcgcct ggcttattcc
      661 agcaaacgcg cggtcgaaga cactctgcaa gctttcaggc gcagcgatca ttggctcttc
      721 gtttcgggct tgggttcttc aggtttaggc tcggctttgg ctgcaggctt gggcccggct
      781 ttggcctcag gcttaggctc cgctttggcc tcaggcttag gctccgcttt ggcttcgggc
      841 ttgggctcgg ttttggtctc aggtttgggc tcagctttgg cttcaggctt aggctcggct
      901 ttggctgcag gcttggactc ggctttggcc tcaggcttag gctcagcttt ggtttcaggc
      961 ttaggctcgg ctttggcttc aggcttgtcc ttctctttca gaagttcgcc gacgcccttt
     1021 tccagcgacg cgaggtaatc ggcgatgctc caagccactt ttcgtccatt cgtggtgagt
     1081 tcgatcccgc tgaccaggtt cggcgcggtc tcgaaccgga cgcggatttc agccgagaag
     1141 gtttcattga gcgcattttg tatcgccgcg cgttgctcca caggcaggtc aaacgcgctg
     1201 cgcacgagcg ccgggccgga cgctgttttg agggcctcgg cgaggccttc tttcgccttg
     1261 ccgtccatct cgcgcaagcg gcgagtgaac acctcgccca tgcgttcttc caggctcgtc
     1321 gtggcgagat ccgtcaacgc ttttcgcgcg atggcaaaca cttcctgttg ggcccggcga
     1381 ctgatcgctt gatttaggtt atgggcgtcg tttctcagcg tttcctgtcg cttggcactc
     1441 aaggcgtcgg ccgccttccg tgcttcgtcg atgagccgct gacgttcggc tttcgcctca
     1501 tccgtcgcgg cgctcatgag agcggcgcgc tgctggtcga actcctcatt cttcttctgg
     1561 aactcgtcgc gctccttttg ggcttcggcc tttttcgcgt cggcgtccgc gagttctttg
     1621 gcgattcgtt tttcccgcgc gtcgatggcg ttgagaatgg gcttataaag aaagcgcttc
     1681 atcaaccaca ccaggatgag gaagttgagc gcttgcgcac cgatggtgaa ccagtcgatc
     1741 agcataggtt cattttcctg tcgtttgggc gatgacgtga ttccagaacg ggttggcgaa
     1801 gatgagaatc atcgagacca cgaagcagta gatggccgtg gattcgatca tcgccaaacc
     1861 gacgaacagg gtccgggtga tggtggcgga ggcgtcaggt tgctgcgcca gcgcagtgag
     1921 cgccgtcgcg accgagcgcc cttcgcccag cgcgggcatc atacacccaa agctggtcgt
     1981 gaggccggcg gtgacgattg aggtgactgc gatgagggtc atgttatcca taatatgtcc
     2041 tttggttggt tggttgttgt ttcaggtttt gggcactggc tccg
//
LOCUS       DATMGX010000506         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_177307, whole genome shotgun sequence.
ACCESSION   DATMGX010000506 DATMGX010000000
VERSION     DATMGX010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177307"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VNR65_08205"
     CDS             <1..617
                     /locus_tag="VNR65_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18693.1"
                     /translation="RKAGEGFGQEADVPAAVADDELVRERATVAQSFDPAEIAHGQLS
                     KIPPPIALAALPLIVVIAVNLLMSFWVLPGLDASFLAEERWGKTSLSAVGGVWAVIVA
                     LAAAVVVLVAVNRRRLPSLRQSIDAGANASVLPAFSVASLVGFGAVVAALPAFAMVRD
                     WVLSIGGGPLVSLAVATNILAALTGSASGGLVAVVLLGSAVGSF"
     gene            805..1377
                     /locus_tag="VNR65_08210"
     CDS             805..1377
                     /locus_tag="VNR65_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HWJ18694.1"
                     /translation="MVISPTAEPTAGGEPTPKSWWLYLLLGIVLVIGGVIVLGDVTVA
                     SVISAIVIAWAVLIVGVFQVVHAFSATGWKGFLLDLLLGALYIAAGYILLANPLTATI
                     KLTLILGIIWLVSGLLRIVLAGVLWRDAGWILLLSGIIGVLAGGVIISEWPQSGLWVL
                     GLCLGVDLIFHGFAWISYSFSLPSRRPQAA"
     gene            complement(1417..>2505)
                     /locus_tag="VNR65_08215"
     CDS             complement(1417..>2505)
                     /locus_tag="VNR65_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWJ18695.1"
                     /translation="AGLLAKTALFPLHLWLPPAHASAPAAASAVLSALVVKGPFFIAV
                     RLWFDVMPNFPAPTARQLLAALGAAAIVFGSILALRQIRLKLLIAYSTLAQIGYLFLM
                     FPLGTGTASHNGIALSGGILQAMSHATAKAAMFMSAGLIYAALGHDRIASLSGAARLL
                     PITVLAFALGGLALMGLPASGAYLAKDLLLQASDETAQWWWAATIQAGGIFTAAYLFL
                     VLANALTPASEPKRLRAAIPRSQEAAALALALCSLFLGLFPWGDYLPMPDGSPPNPFT
                     LKALSTVLWTMLAGSVLAIFMARWEHPLERLPRVGRFFLWASPIRRVALGLAKMFERI
                     DTVTAQWPVAGISLLALATVFGVALWAG"
BASE COUNT          458 a          782 c          742 g          523 t
ORIGIN      
        1 tgcgtaaagc tggcgagggt ttcgggcagg aggcggacgt tcccgccgcc gtcgccgacg
       61 acgaacttgt tcgcgaacgc gcaaccgttg cgcaaagctt cgaccccgcc gagattgccc
      121 acggccagct cagcaaaatt ccaccgccca tcgcgcttgc cgcactgcct ctcatcgttg
      181 tcatcgccgt caaccttctg atgtcgttct gggtgctacc tggactggat gcttcgtttc
      241 tggctgagga acgctggggc aaaacctcgc tctcggccgt gggcggggta tgggccgtga
      301 tcgttgcgct tgccgcagcc gttgtcgttc tcgtcgccgt caatcgtcgg cgcctaccat
      361 cgttgcggca gagcatcgac gcgggcgcca acgcatccgt cttgccagcc ttcagcgtcg
      421 cgagccttgt cggtttcggc gctgtcgttg ccgctctgcc tgcctttgca atggttcgcg
      481 attgggtact ttccatcggg ggcgggccgc tcgtttcgct tgccgtcgca acgaacattc
      541 ttgcggcgct gacaggatcg gcttcgggag gactcgtcgc ggtcgtcctt ttgggctcgg
      601 ctgtaggttc gttctgacag atcgccagag gccttccgtc cctttcttaa agaaatggtg
      661 atcgcctttt ttctctagtc gtggtatctg gaaagctcag tacaggccgt tatcattttc
      721 tccgcatttc ccaatgtcag cgcatagaaa agcgcgtaac ggcttcccct tgcgccgaac
      781 ccaatacttt ccaagaggtc tcagatggtc atttccccta cagcggagcc gacagctggc
      841 ggcgaaccca ctcctaagtc ctggtggctt tatctgctcc taggcattgt tcttgtcatc
      901 ggcggtgtca tcgttcttgg cgatgtcacc gtagcctcgg tcatcagcgc catcgtcatc
      961 gcctgggcgg ttttgatcgt cggagttttc caggtcgtcc atgccttcag tgcgacagga
     1021 tggaaagggt ttctcctcga tctgctgctc ggcgccctct acattgcggc cggctacatt
     1081 cttctggcca atcccctgac ggcaacaatc aagctcacgc ttatcctcgg catcatttgg
     1141 cttgtttcgg gactgttgcg gattgtgctc gccggcgtgc tgtggcgaga tgcggggtgg
     1201 attctcctcc tctccggcat tattggcgtc cttgccggcg gtgtcatcat atccgaatgg
     1261 ccacaatcgg gcctttgggt cctgggcctc tgccttggcg tcgacctcat cttccatggc
     1321 ttcgcctgga tatcatactc attctcgctt ccgtcccgcc ggccccaggc tgcgtagctg
     1381 attgaatttc tgacgccgat gtttctgtca tcggcgtcag ccagcccaca aggcaacgcc
     1441 gaacacggtc gccaacgcga gaagggaaat acccgccacc ggccattgcg ccgtgacggt
     1501 atcgatccgc tcgaacattt tcgccaaacc aagggcgacg cgccggatgg ggctcgccca
     1561 aaggaaaaag cgcccaaccc gaggcaggcg ctcaagcgga tgttcccagc gcgccatgaa
     1621 tatcgcgagc acgcttcctg ccagcatcgt ccagagaacc gttgagagcg ccttcagtgt
     1681 gaatgggttt ggcgggctcc cgtccggcat cgggagatag tccccccaag gaaaaagccc
     1741 caggaacagc gagcaaagcg cgagcgccag cgctgctgct tcctggctac gcggtatggc
     1801 ggcgcgcaat ctcttcggtt cgctggcggg cgtcaacgcg ttagcaagca ccaggaaaag
     1861 gtaggcggcg gtgaaaatgc cgccagcctg aattgtcgcc gcccaccacc attgggctgt
     1921 ttcgtccgat gcctgcagca acaaatcctt tgcgagataa gcgccgcttg caggaaggcc
     1981 catcagcgcc agaccgccca gcgcaaaagc aagcactgtg atgggcaaga ggcgcgccgc
     2041 gcccgaaagg ctcgcgatac ggtcgtgccc cagtgccgcg tagataaggc cagccgacat
     2101 gaacatcgcg gccttcgccg tggcgtggga catcgcctga agaataccac ccgataaggc
     2161 gatgccgtta tgcgacgccg ttccagtgcc gagcggaaac atcagaaaga gatagccgat
     2221 ctgcgccagc gtcgagtagg cgatcagaag cttcagtctt atctggcgca gcgcaaggat
     2281 gctgccgaag acaatcgcag cggctccgag cgcggcgagc aactgccttg cggttggcgc
     2341 cggaaagttc ggcatcacat cgaaccagag gcgcaccgcg atgaaaaatg gccccttcac
     2401 gacgagcgcc gacaacacgg cactcgctgc ggcaggtgcc gaggcgtggg ctggcggaag
     2461 ccacagatgc agggggaaaa gcgccgtctt cgcaagaagg cccgc
//
LOCUS       DATMGX010000507         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_173665, whole genome shotgun sequence.
ACCESSION   DATMGX010000507 DATMGX010000000
VERSION     DATMGX010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173665"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VNR65_08220"
     CDS             <1..356
                     /locus_tag="VNR65_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWJ18696.1"
                     /translation="YQLHLLFDAHYEEQAELVDTIAERIMSLGGVSVAMAHDVAEMTM
                     IRRPPKGRETPAMQITRLVEAHHHILAESRQAAKAAADAGDDGTNDLLVSDVIRLNEK
                     QTWFLAEHLVATRLE"
     gene            998..1633
                     /locus_tag="VNR65_08225"
     CDS             998..1633
                     /locus_tag="VNR65_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HWJ18697.1"
                     /translation="MTRLQARLTLFTVMAIFAATASNALFLQDRARLLNGSGLPSTAV
                     SLTQLSADKAAPDNAGASALLEEDPRAPRLHIALQRELGRRGYANQLQVRPNGLRLAV
                     LAYEFDNSLPLTGEPTEALLKRVLFDGNQGPRGSFSDRAELNTKLVTEAQRMLAGLGF
                     FRGTFSGRIDVWTSNALRDFERHRGIPLTGRLTDQTLLELISYSGQPIRLS"
BASE COUNT          423 a          602 c          556 g          435 t
ORIGIN      
        1 tctaccagct tcatcttctc ttcgatgcac actatgagga gcaggccgaa ctggtggaca
       61 ccatcgccga gcgcatcatg tcgcttgggg gcgtttctgt ggcgatggct cacgatgtgg
      121 ccgaaatgac gatgatccgc cgtcctccga agggccgcga gacgcccgcg atgcagatca
      181 cgcgtcttgt ggaagcgcac caccatatcc tggctgaatc ccggcaggcg gccaaggcgg
      241 ccgcggatgc cggggacgac ggtacgaacg accttctcgt cagcgatgtc atccggctga
      301 acgagaagca gacgtggttt ctcgcggagc atcttgtcgc gacgcgcctc gaatagcagc
      361 cgggcgaccg gccttgcccc catcagaacg tggctggacc gcgccagggc aactccctgt
      421 cggaggagct aaggctattc tgccatttcg ctacaggctg aggcgaagcg tcgctccgcc
      481 ccggctttcg agcgactgtc cgttcagtga ctgaggcgtg ctcctcgcca cccgcgctct
      541 gttgggccag ccaggtctga agcagttccg cgccacttct gacccttgtg agaaggctcg
      601 tcatcgacat ccgcatgtcg tcgcatactt gggggttccg ttggcaaaac cccctcatat
      661 cgcttacggt ctgttcagct gatgagatca atcgacgatt gttttcatga gagctcggaa
      721 tcagcaaaca gaccagcatt atccaaaatg ccagccggag taaaaaaaac ataacgcatc
      781 acccatagtt actcctaacc tcgatatcct aacgctcagg aacaaaaata aaagcgaagg
      841 tggatgctac aattttaaga gtattttatt ctgtggtggc ggaaacaact gaggcttaat
      901 ctgcaaataa ggatactact cttggtcaat attaacagcc gctttctgcg cacaaagcac
      961 ctttctttaa atggagcttt gtgcgattat tccggccatg acccggctac aggcgcgcct
     1021 gaccttgttc acagtaatgg cgatcttcgc ggcgacggcg agtaatgcgc tgttcctgca
     1081 ggatcgcgcg cgtttgctga atgggagtgg ccttcccagc accgcggtct cgctgacgca
     1141 gctttcggcc gacaaggcag cgccggacaa tgctggcgca tccgcgttgt tggaagaaga
     1201 tccgagagcg cctcgcttgc acatagcgct ccagcgggag cttgggcggc gcggctatgc
     1261 gaaccagctc caggtgcgtc caaatggcct tcggctcgct gttctcgcct atgaattcga
     1321 taatagcctg cctttgaccg gggaacccac cgaagccttg ctgaagcgcg ttcttttcga
     1381 tggcaaccaa ggccctcgcg gctctttttc cgaccgcgcc gaactcaata cgaagctcgt
     1441 gacggaagcc cagagaatgc tcgcggggct tggcttcttc cgcggcacgt tctccggccg
     1501 catcgatgtc tggacctcga acgccctccg ggatttcgag cggcatcgcg gcatcccgct
     1561 gacgggccgc ttgaccgatc agacccttct cgagctgatt tcctactcgg gtcagcccat
     1621 ccgcctgtca taagcgaacc agcgccgttg ttgccgacgt gacaacggct cgctcccacc
     1681 gtccttccgg atcgtaacga attgaaacgc ctatcttttt gtgatcccgg ctcgcgcttc
     1741 gcttggccgg gatgacgagg gcggagcgag cggcgtccgg catgacaatc cgttggtcag
     1801 attagcgaaa tttgcttaaa gccaattccc acgcgcagag ctgatgccaa aaaggcacgc
     1861 ccccgcgcct gaacacggca atcgcaaaga gttacacttt ttgcggtggc gtccgaggta
     1921 aacaatccct taacagtcac aattcaaagc gttctttatt gcagcgattc tctttgtttg
     1981 agggtgggaa gagccggacc gaaactcatt caaatc
//
LOCUS       DATMGX010000508         3287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_351867, whole genome shotgun sequence.
ACCESSION   DATMGX010000508 DATMGX010000000
VERSION     DATMGX010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351867"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..265)
                     /locus_tag="VNR65_08230"
     CDS             complement(65..265)
                     /locus_tag="VNR65_08230"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HWJ18698.1"
                     /translation="MAQKVIDVVGTSKESFAKAAENAVAEAAKTVRGMKWARVAELEI
                     ELDGKKIVKYRTTTKIYFDIEK"
     gene            433..1200
                     /gene="xth"
                     /locus_tag="VNR65_08235"
     CDS             433..1200
                     /gene="xth"
                     /locus_tag="VNR65_08235"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWJ18699.1"
                     /translation="MFKVASWNLNSVRSRLTHVTAWLKAREPDVLLLQELKGTEFPSE
                     AFKALGYESVAVTQKAYNGVAILSRHPIETIAKALVGDDSDSHARFLEVIIEGIRIVN
                     IYAPNGNPVGSDKFAYKLAWMDRLNQQMIRWKQDDLPTLIGGDFNVIPEDIDCHKPSS
                     WEHDALFQPEPRARYRAMLGLGYMDAFRSLHVGEVGHFTFWDYFRQAFKNNRGIRIDH
                     FLLSPKLANRLESCEIDKGPRALEKPSDHTPIIVTLR"
     gene            1961..3178
                     /locus_tag="VNR65_08240"
     CDS             1961..3178
                     /locus_tag="VNR65_08240"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWJ18700.1"
                     /translation="MNTGIEKTSQKLAGEAGHSGPEIRSDVHVLIEPRERGGIDIELE
                     SRVKPYYGDAIRRQAEEVLEALGVRHARVVIHDGGALPFVIAARLESAARLAGLGEGT
                     RVLPNRLSTPEPSARDRMRRSRLYVPGSEPKYFVNAALHGADAIILDLEDSVHPNEKD
                     AARVLVRNALRAVDFLRCERMVRINQLPLGLKDLEEIVPESPDLILLPKVETPYQVID
                     ASQKIAEIRATFGIDRRIWLMPILESALGIEKAYEIATASEEVTALTIGLEDYTADVG
                     VVKTASGAESLFARQRVVNAAHAAGIQAIDSVFGDVGDIDGLRTWALNSRATGFEGMG
                     CVHPSQIPVIHEAFAPTAAEIEKARKIVDAYNEAQAKSLGVVSLGSKMIDAPVVRRAL
                     KLMAQAEAMQPLK"
BASE COUNT          743 a          875 c          946 g          723 t
ORIGIN      
        1 gatgcgcccc gtgctcaatg agtccggggc gccttcgttt gcaaatttca catccgaaca
       61 atccctactt ttcaatgtcg aaatagattt tcgttgtggt gcggtattta acgatcttct
      121 tgccgtccaa ttcgatttcc agttccgcca cacgcgccca cttcatgccc cgaacagtct
      181 ttgcggcttc cgccaccgca ttttccgccg cctttgcgaa gctctctttg gatgttccca
      241 ctacatcaat aactttttgc gccatttgcg gttctccttt gattctgatt cagttcggat
      301 tgtaccgcta ggtcgagagt gactttgccg caaaacgttg tgattgtgaa aggagaggtt
      361 cagagtgggc tttcttgtag caggccagtt gagagcagcg gtatccgtcc cgtaacgcta
      421 gaatgtggat cgatgtttaa agtcgctagc tggaatttga attctgtgcg ctctcggctg
      481 acccacgtca cggcatggct gaaagctcgc gaaccggatg tgttgctgtt gcaagaattg
      541 aagggcacgg aattcccgtc cgaagccttc aaggcgctcg gctatgagag tgtggcggtg
      601 acgcagaagg cctacaacgg ggttgccatt ctgtcgcgtc atccgataga gacgattgcc
      661 aaggctctcg tcggagacga ttccgacagc catgcgcggt tcttggaagt aatcatcgag
      721 ggtatccgca tcgtcaatat ttatgctccc aatggcaatc cggtagggag tgacaagttt
      781 gcgtacaagt tagcttggat ggatcgactg aatcagcaaa tgatcagatg gaagcaggac
      841 gatctgccga cgctaatcgg cggcgacttc aacgtcattc ctgaggatat cgactgccac
      901 aagccgtcat cgtgggagca cgatgctttg tttcagccgg agccacgcgc ccgctatcgt
      961 gcgatgttgg gtctgggcta tatggatgcg ttccggtcgt tgcacgtggg cgaagtaggc
     1021 cacttcacgt tttgggatta ctttcggcag gcgttcaaaa acaatcgagg catacgaatc
     1081 gaccacttct tattatcccc aaaactggca aatcgactcg aaagttgcga gatcgacaag
     1141 ggaccgagag cgttggaaaa gccgtctgac cacaccccca taatcgtgac gctccgatag
     1201 acacactcga tctcgtccat ggtgcgtttc acgtccatgc gtcagaatat ccaacatgag
     1261 cgtggcattc gtcaaacgga gcacgacggc agcagatggc atgcaatacg ttgttttcga
     1321 tgtagagcag tgcgaacggc acggaaacgg cacgggatgg cagagatctt ttttcaagcg
     1381 attcaatcga cgctttcgct tctctcacgc caatattctg ctccagcaga cggacgcgca
     1441 accctccgca ttagagtgaa tgtctcattt agtgcaggga cgcgccccca cacgaagctt
     1501 cacgccgcgc taaagcagag ctgttaaacg gcgttccggg caatccagtg ttccaaggca
     1561 ttaggttcgc tgaattttgt cccgcacaag gctgaatgcg tgctcaacga aacgctggca
     1621 cttttcgttt gcatttggac gcaatggttt atcatttggt gacgctgatc actccagcgt
     1681 gacgcaaaag tcgcaagaga ccccagtagg caaaaagagg caggggcagc gagttcgaga
     1741 tccgtttcac gacggcgatg cgtgacgcaa agggtgggcc tatgagacgg atcctcagca
     1801 atttgaagtg ctcgcgatgt ggctttgaga agaaccatcc cgtgagcttg aactggattg
     1861 cctgtgggat tgacctcgga aatcaatctg cgcctacccc aactcgaccg aacacgatag
     1921 cttctgcgac gagaatcggg ttcgtggcca cacagaattc atgaatacgg gcatcgaaaa
     1981 gacatcgcaa aaacttgcag gcgaggcggg acacagcggg ccggagattc gctcggacgt
     2041 gcacgtgctg atcgaaccac gcgagcgcgg cgggatcgac atcgaactcg aatcacgcgt
     2101 caaaccgtac tacggcgacg ccatccgccg gcaggctgag gaagtgctcg aagcgctcgg
     2161 cgtaaggcac gcccgggtgg ttatccacga tgggggcgca ctgccattcg tgattgcggc
     2221 gcgcctcgaa agcgcggcca ggcttgcggg actgggcgaa ggcactcgtg ttctgccgaa
     2281 caggctgtcg actcctgagc cttctgcgcg cgatcgcatg cggcgttcgc gactttatgt
     2341 tcctgggagc gagcctaagt atttcgtcaa cgccgcgctg cacggagcag acgcgatcat
     2401 tctcgatctc gaagattcgg ttcaccccaa cgaaaaggat gcggcgcgag tgctggtgcg
     2461 caacgcgctg cgcgccgtcg actttctgcg atgcgagcgc atggtacgca tcaaccagct
     2521 tcctctcgga ctcaaagacc tggaagagat tgttcccgaa tctcccgacc tgattcttct
     2581 tcccaaggtt gaaactccgt accaagtgat tgatgccagt cagaagatcg cggaaatcag
     2641 agctactttc ggcattgacc gccgcatctg gttgatgcct attctggagt ctgccctggg
     2701 aatcgagaag gcatacgaga ttgcgaccgc aagcgaagag gttacggcgc tcaccattgg
     2761 attggaagat tacaccgcag acgtcggcgt ggtgaagacg gcttcggggg cggagtcgct
     2821 ctttgcgcga cagcgcgtag tcaatgcggc ccacgccgcc gggatccagg ccatcgattc
     2881 ggtctttggg gatgtcggcg atattgatgg cttgcgtacg tgggctttga attctcgcgc
     2941 gacgggattt gaaggcatgg gatgcgtcca cccgtcgcag attccggtaa ttcatgaagc
     3001 atttgcgccg acggcagcag aaatcgaaaa agcacgaaag attgtcgacg cctacaatga
     3061 ggctcaagca aaaagcttgg gcgtggtcag tctcgggtcg aagatgatcg acgctcccgt
     3121 cgtgcgacga gccctgaagc tgatggcgca agcggaagcg atgcaaccgc tgaagtgaac
     3181 gctgagcaag cacgagtgcg gagtctgatg gacattctcg aaggaacgaa actcatcctc
     3241 aacgccgccg gtcgcatggt tccggagatg gcaaacggtc gccggca
//
LOCUS       DATMGX010000509         3602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_99204, whole genome shotgun sequence.
ACCESSION   DATMGX010000509 DATMGX010000000
VERSION     DATMGX010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99204"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VNR65_08245"
     CDS             complement(<1..663)
                     /locus_tag="VNR65_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWJ18701.1"
                     /translation="MPVPRLIIFDFDGVIADSETLACAVLAEIISELGCAITTDDAIR
                     LFTGLRAQECYATIEKIIGRPLPQDFVSILRKRRSASFERELTAVEGVREYIRAFAST
                     PQCIASQSSLEYLAASLQIIGLAESFQDAVFSAAGMERGKPHPDIYLHAAEKMGVDPA
                     RAIVIEDSVLGVQAGVAAGATVIGLLAGSHILRGHEQRLCEAGAHYIARTFAEATDVT
                     RSL"
     gene            1108..2172
                     /locus_tag="VNR65_08250"
     CDS             1108..2172
                     /locus_tag="VNR65_08250"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWJ18702.1"
                     /translation="MSIKHNTPPATSEARHWAQILNCYREPSPARSIAELAITALPLA
                     ALWLASWFAFSWGYWWASLLIAIPAAGFLVRLFMIQHDCGHGAFFSKRWANDWTGRVI
                     GVLTLTPYGHWRHTHGVHHATCGNLGRRGIGDVATLTVREYCALSRWGRLKYRLYRHP
                     LVMFGFGPSYLFLLQHRLPLGLMRGGGWQPWASTMSTNLAIVSIAAVLAWLIGIKAVL
                     LIHLPVVLLAATIGVWLFYVQHQFEHTTWEPEGEWNFHDAALHGSSHYDLPAALRWFT
                     ANIGVHHVHHLCSRIPYYRLPQVLRDHPELRNVSRLTLLESFRCVRLVLWDETQRRLV
                     SFRDLRACPVTRDRGEWGQA"
     gene            complement(2231..2872)
                     /locus_tag="VNR65_08255"
     CDS             complement(2231..2872)
                     /locus_tag="VNR65_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWJ18703.1"
                     /translation="MFALTRRTITALAVSLGLSAIAGLAASPAGADQKTLAQDLRAGG
                     FVIVVRHGATFADQADTDPLNFDNIAAQRNLNDKGKAQAKAFGDAIRQAGIPVGEVYT
                     SKYNRAYETAVIAGFKDIEKTADLTEGGLVVSPNENNRRVAALRKMLGTAPKPGVNTI
                     LITHKPNIVDALGKDWFEVKEGEASIFRPENGSYQLVARVQMDEWPRIAMAVN"
     gene            complement(3137..>3602)
                     /locus_tag="VNR65_08260"
     CDS             complement(3137..>3602)
                     /locus_tag="VNR65_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HWJ18704.1"
                     /translation="ARAGYLFDAGPVRVGPIAGINYTLADIQGYTENGDSLLTMMVNE
                     QNVDSLTGNAGLQMRFPFELWGDLYSPFINVTAEQDLLGSSRLVTTTLVTAPLLPVLT
                     PVPGESSIYGKIAAGVAARIGGTVSATVNAETTFAREGGNGLFVSGGIKVGL"
BASE COUNT          718 a         1096 c         1020 g          768 t
ORIGIN      
        1 tagagaacgc gttacgtcag tggcctcggc aaaagttcgt gcaatgtaat gcgccccggc
       61 ctcacacaac ctttgttcgt ggcccctcag gatgtgtgat ccggccagaa gcccaataac
      121 ggttgcaccg gctgccactc cagcctgaac gccgaggaca ctatcctcaa tgacgatggc
      181 tcgcgctgga tcgacgccca ttttttctgc ggcatgcagg tagatgtcgg gatgaggctt
      241 gcctctttcc atgcccgcag cgctgaaaac agcgtcttga aaactctccg ccagccctat
      301 gatctgcagg gaggcggcca gatattcaag cgagctttgc gatgctatgc attggggcgt
      361 cgaggcgaag gcccttatgt attcccgcac cccttcaacg gccgtcaatt cgcgctcgaa
      421 cgacgccgaa cggcgctttc tcagtatgga tacaaagtcc tgcggcaagg gccggccaat
      481 aattttctcg attgtcgcgt agcattcctg agcacgcaac cctgtgaaga gccggatggc
      541 atcgtctgtt gtgatggcgc atccaagctc gcttatgatt tcagcaagta cggcgcatgc
      601 gagcgtctcg ctatcggcaa taacgccgtc aaagtcaaag atgatgagtc gtggaacagg
      661 catttttcct ctatcggctg agacagggga aaccttgcgg catcccgcac ttctccagcc
      721 tcatgatttt tctgtccagc ccaagcagca aacaccaaaa gaccccggcc ggcggacgga
      781 gtctcagttg gagatggaaa accctgggaa acacttggca gcgtgggtga ttccccgctc
      841 tccccgcaat tcacgtttta ttacggcagg attcaactca gacctctgaa ccggtttaac
      901 ctcagcggca aaacgctctt caagcacgat ctcgtcattc gggcaggaag ccgccatata
      961 caaaatggcg ctccttttaa gcttgcgcgc acctccacga gccctctgcg aggtaggctt
     1021 ggaaatacca agagaaaacg gccataatcg gctaggcgcc gggttgcgtg ccgatccgat
     1081 gggcgccaat cgccaatagg cattatcttg agcatcaagc acaacacgcc tcccgcaacg
     1141 agcgaggcac gtcactgggc acaaatcctc aactgctatc gcgagccgag ccctgcacgc
     1201 agcatcgcag aactcgcgat aacggcgctg ccgcttgcgg cgctatggct cgcgagctgg
     1261 ttcgctttct cctggggtta ttggtgggct tcgctgctca tcgccatccc cgccgccggt
     1321 tttctcgtgc gcctgttcat gatccagcat gattgcggtc atggcgcctt cttctcaaaa
     1381 agatgggcga atgattggac gggccgcgtc atcggcgtcc tcaccttgac gccttacggc
     1441 cattggcgcc acacgcatgg tgtccaccac gccacgtgcg gcaatctcgg ccgccgcggg
     1501 atcggcgatg tggcgacgct gaccgttcgc gaatattgcg cgctttcgcg ctggggccgg
     1561 ctcaaatacc ggctttaccg ccatcccttg gtgatgttcg gttttggccc tagttatcta
     1621 tttcttctgc agcatcgcct gcccctgggg ctcatgcgcg gcggcggctg gcagccttgg
     1681 gccagcacaa tgtcgaccaa tcttgcgatc gtatcgatcg ctgccgtcct cgcctggctg
     1741 atcggcatca aggcagtcct gctcatccat cttccggtcg tgctgctcgc cgccacgatc
     1801 ggcgtttggc tgttctatgt tcaacatcag tttgaacaca cgacttggga acccgagggt
     1861 gagtggaatt ttcacgacgc ggccttgcac ggcagttcgc actacgatct gcccgcggct
     1921 ctccgctggt tcacggccaa tatcggcgtg catcacgtcc atcacctctg cagccgcatc
     1981 ccctactacc gtctgccgca ggtgctgcgc gaccatccgg aactccgcaa cgtcagccgg
     2041 ctgacgctgc tcgaaagctt tcgctgtgtc cgccttgtat tgtgggacga gacgcaacgc
     2101 cgtctggtgt cgttccgtga tctccgggcg tgcccggtta cccgagaccg cggcgaatgg
     2161 gggcaggcct gaagtaaagt aaagaacgcg ctgccggcag cggcaaagaa aaaccagacc
     2221 gagatgattg ctaattcacc gccatggcga tgcgaggcca ttcatccatt tgcaccctgg
     2281 cgacgagctg gtaactgccg ttttcgggac ggaagatcga agcctcgcct tctttcacct
     2341 cgaaccagtc cttgccgagc gcatcgacga tgttcggctt gtgcgtgata agaatggtgt
     2401 tcacgcccgg cttcggcgct gtgccgagca tcttccgaag agctgcgacg cggcggttgt
     2461 tttcgttcgg ggaaacgacg agaccgcctt cggtgagatc ggccgtcttc tcgatatcct
     2521 tgaagcctgc gatgacagcg gtctcataag ctcgattgta cttgctggta tagacctcgc
     2581 cgacgggaat accggcctgg cggatggcat ccccaaaagc cttcgcctgc gccttgccct
     2641 tgtcattgag attacgctgg gccgcgatgt tatcgaagtt gagcggatcg gtatcggcct
     2701 gatcggcaaa agtggcgccg tggcggacga caatcacaaa ccctccagca cgcaagtcct
     2761 gcgcgagagt cttctggtca gccccggccg ggctcgcggc aaggccggct atcgcggaca
     2821 ggcccaaaga gacagcgaga gctgttatgg ttcttctcgt cagagcaaac atcgtacaac
     2881 tccttgaaag aaaacggtaa gcggcgggat gctgccccgc tcagcgggca tcacctgcat
     2941 ctccccacaa gactcgcagt gcgccggctt tattcccccc caagacttgg tgatgatgaa
     3001 ataccttgtg tttttctgga tggccgggtc aagcccggcc atgacaggtt agcgagaaat
     3061 tcgccaaggt tttcaaggct tcttctgcaa gcgtgaacag ccgggtaatc aagttgcggc
     3121 atgacttgga gttatcctac aatcccacct taatgccgcc gctgacgaaa aggccgtttc
     3181 cgccctcgcg tgcaaaggtg gtctccgcat tcaccgtagc gctgactgtc ccgccaatcc
     3241 tcgccgcgac gccagcagcg atcttgccat aaatgctgct ctcgcccgga acgggggtca
     3301 gcacaggaag cagaggggcc gttaccagcg tggtcgtgac caaacggctg gaaccaagca
     3361 aatcctgctc ggcggtgacg ttgatgaacg ggctgtagag atccccccaa agttcgaagg
     3421 gaaagcgcat ctgaagcccg gcgttgccgg tcaagctgtc aacattctgc tcgttcacca
     3481 tcatggtgag gaggctgtcg ccgttttcgg tgtatccctg aatgtcggcg agcgtgtagt
     3541 tgatgccggc gatcggccca acgcggactg gcccggcgtc aaagaggtag ccggccctgg
     3601 cg
//
LOCUS       DATMGX010000510         3822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_75147, whole genome shotgun sequence.
ACCESSION   DATMGX010000510 DATMGX010000000
VERSION     DATMGX010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75147"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VNR65_08265"
     CDS             complement(<1..283)
                     /locus_tag="VNR65_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18705.1"
                     /translation="MAIKIKLPDGRASARPASGRNSRDGRGRPLRTQHFSIADPLVKL
                     LASGFLIAGVLFMVVFTYFYVKYENIVDRRMSGPIFSNAARIYARPHTVW"
     gene            complement(598..2154)
                     /gene="hutH"
                     /locus_tag="VNR65_08270"
     CDS             complement(598..2154)
                     /gene="hutH"
                     /locus_tag="VNR65_08270"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HWJ18706.1"
                     /translation="MQALHINGNDLTLDDVRQVVHERRPILLQSEARIAVERARAVVE
                     DLLENDRVAYAVNTGVGKLADLRISLDDIRQLQIKLLRSHAAGVGEPLAEDVTRAMVL
                     LRANSLAKGFSGVRPAVIDTLCEMLNRGVHPVIPSQGSVGASGDLAPLAHLALVLIAE
                     GEATFRGKRMAAAEALKQADIEPLALQAKEAISLINGTQGMLAVGLLATLQANILADT
                     ADVLGALTVDALKGTDAAFDKRVHQARPHAGQSLVAENLRRMLEGSLIRESHRECQKV
                     QDAYSLRCIPQVHGAVRDTLAHCRKIFEIEMNSAVDNPLVFPEERKVGAPKRGVGSTV
                     PGDILSGGNFHGEPLAFALDFLAIALSGLAGISERRLERLVNPALNEGLPPFLAPGAG
                     LNSGFMMPQVTAAALVSENKVLAHPASVDSITTSGNQEDYVSMGMIAANKLLRVVTNT
                     RNVLAIEALAVAQALDFRAPLKTGKRGQAAHAAIRSVSPTITEDRIFTPDFAKVAELI
                     ASGKLAEVLR"
     gene            complement(2285..2695)
                     /locus_tag="VNR65_08275"
     CDS             complement(2285..2695)
                     /locus_tag="VNR65_08275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWJ18707.1"
                     /translation="MYETKLRVRYAETDQMGVVYHSNFVIWFEVGRVEMLRQLGFTYR
                     EMEKQDGTHIAVVDMRCRYKAPARYDDMVTIRTRLVNVRNSLLHFGYEVVRDEDGALL
                     AEGETVHLVVDSEFNRIPLPQRYMASFLKAVGRN"
     gene            complement(2832..>3822)
                     /locus_tag="VNR65_08280"
     CDS             complement(2832..>3822)
                     /locus_tag="VNR65_08280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWJ18708.1"
                     /translation="SYAAYPVMSEAAKERGRKLFEMFKAGQASAIWATYPAENRKRAE
                     EERKFVATLAQLKTKLGTETKVDAENIVPYMLKRATVYSRLSEFANSKAPVMTIIVID
                     ENGETEGFNLRAQPLPPEGHNAGYKDTAKLKLPFSGQWLVYQGGHGVFNNGYQMSEDQ
                     QFAMDFVLLKNGIPFEGDATTNEQFYCFGQPVLAPADGTVVRVVNDIGDNAPGKPSQE
                     TPNGNMILMSHGNNEYSLLTNLKQNSVKLKHGDAVKQGDPVGECGNSGGSAVPKVHYQ
                     LQNSARFPRVESLPAQFVDYIADGKPVAVGEVVKGQTVSNGPTSSTSQPAEKK"
BASE COUNT          737 a         1164 c         1100 g          821 t
ORIGIN      
        1 cccagacagt gtgtgggcgg gcgtagatcc tggcagcatt gctgaagatc ggtccagaca
       61 tgcggcggtc cacgatgttc tcgtacttca cgtagaagta agtgaacaca accatgaaca
      121 ggacgccggc aatgagaaag cccgaggcga ggagcttcac cagcggatcg gcgatcgaga
      181 agtgttgcgt gcggagcggc cttccccgcc catcgcggga attcctcccg gatgcgggac
      241 gggccgaagc cctgccatca ggtaatttga ttttgattgc cacgtttatg aatacgcaga
      301 gaagccgctc tttctaagac ggcgatgcgg cgctttccgt tatctgagat gccactttcc
      361 cgggaaaatc ctcgaaactt ttggcatcca aggggcgcgg cccaccctcg ggcgcagcaa
      421 aactgacgtc cgagtcgacg aggtttaagg acgactccag agcagttctt attgtaaagc
      481 agggagcgcc cgccgggtta gcggagtgga caggtggtta ctggtgtggg ttgtacggcg
      541 cttcgggatc tgcgtctctc tgacgaaccg acgtctgtag aatcagtaac ttagagacta
      601 ccgcagcact tccgccagct ttccgctggc gatcagttca gctactttcg cgaagtccgg
      661 ggtgaagatt cgatcttccg tgatggtggg agacacggac cgaatggctg cgtgggccgc
      721 ttggccgcgc ttgccggtct tcaggggtgc gcggaagtcg agcgcctggg cgacggccag
      781 ggcttcgatg gcgagcacat tgcgcgtgtt ggtgaccact cgcagcagct tgttggccgc
      841 gatcatgccc atcgagacgt agtcttcctg gttccccgac gtggtgatgg aatccacgga
      901 cgcgggatgc gccagcactt tgttttcgct gaccagcgca gcagccgtga cttgcggcat
      961 catgaatcct gagtttaggc cggctccggg cgcgaggaac ggaggcagcc cttcgttgag
     1021 cgctggattc accagccgct ccagccgtcg ttctgagatg cccgccaacc cactgagcgc
     1081 gatggccaga aaatcgagcg cgaatgccag cggttcccca tgaaagtttc cgcccgacag
     1141 gatgtcgccg gggaccgtcg atcccacgcc gcgcttcggc gcgcctactt tcctttcctc
     1201 aggaaagacc agcggattgt ccaccgccga attcatttcg atctcaaaga tcttgcggca
     1261 atgggccagg gtatcgcgca ccgcgccgtg gacctgcggg atgcagcgca gcgaataggc
     1321 atcctgcacc ttctgacact cgcgatgcga ctcgcgaatc aggctgcctt ccaacatgcg
     1381 gcgcaggttc tccgcgacca gtgactgtcc cgcgtggggg cgcgcctggt ggacgcgctt
     1441 gtcaaacgca gcatcggtac ccttcagcgc atctacggtg agagcgccga gcacatcggc
     1501 ggtgtccgcg agtatgttcg cctgcagagt ggccaacaat ccgaccgcca gcatgccctg
     1561 ggtgccattg atgagcgaga ttgcctcttt ggcttgcagc gccagtggct cgatgtcagc
     1621 ctgcttcagg gcttccgcag ccgccatgcg cttgccgcgg aaggtggctt cgccctccgc
     1681 gatcagcacc agcgcgagat gcgccagcgg agccagatcg ccgctggctc ccacgcttcc
     1741 ctgcgacgga atcacaggat gcacgccacg gttcagcatc tcgcaaagcg tgtcaatgac
     1801 cgcgggacgc accccggaga aacccttggc gagcgagttg gcgcgcagca gcaccatggc
     1861 gcgcgtaacg tcttccgcca gcggttcgcc tacgccggcc gcatgcgagc gcaaaagttt
     1921 gatttgaagc tggcgtatat cgtctagcga gatgcgaaga tcggcgagtt tgccgacgcc
     1981 agtgtttacg gcgtaggcga cacgatcgtt ctcaagcagg tcttccacca cggcgcgcgc
     2041 gcgttcgact gcgatgcgcg cctccgactg caacaaaatc ggacgccgct catggaccac
     2101 ctggcgaaca tcgtcgaggg tcagatcgtt gccgttgatg tggagcgctt gcattttgca
     2161 atagaggtca gtttcgagtt gctaaacgtg cttcacaaag aggcgtgacg tgacgttccg
     2221 cagcaagtct gtccaagacc ctgttcccgc gaattttggc aagcaccgcc gctcgccagt
     2281 cgcctcagtt tcttcctacc gccttcaaaa acgacgccat gtacctctgc ggcagaggga
     2341 ttcggttgaa ttcagaatcc accacaagat gtacggtctc gccctcggcc agcagggcac
     2401 catcctcgtc gcgtacgacc tcgtagccga aatgcaacaa agaattgcgc acattgacca
     2461 ggcgcgtccg aatggtgacc atgtcatcgt agcgggccgg ggctttgtag cggcagcgca
     2521 tgtccacgac ggcgatgtgc gtgccgtcct gcttctccat ttcgcgatag gtgaagccga
     2581 gctggcgcag catttccacc cggcccacct caaaccagat gacgaagttg gagtgataga
     2641 ccacgcccat ctggtccgtc tccgcatagc gcacccgaag cttggtctcg tacacttgcg
     2701 cttccgaagt tgtgcgtagc gactgcctgt cttcggcttt caacccgaca cccaccccac
     2761 tggagacacg gcccatttca accacgaagc cggctgcgca gagaacggca cctgccttgc
     2821 aaccgtagcc cttatttttt ctccgccggt tgagaggtgc tcgaagtagg tccgttgctg
     2881 accgtctgtc cttttacgac ttcacctacg gccactggct ttccgtccgc tatatagtcg
     2941 acgaattgcg ccggcaacga ctccaccctt gggaacctcg ccgaattctg caattggtag
     3001 tgcactttgg gaacagccga accgccagag ttgccacact ctcccacggg atcaccctgt
     3061 ttcaccgcgt ctccatgctt caacttaacg ctgttttgct tcagattggt cagcagggag
     3121 tattcgttgt tgccgtgcga catcaggatc atgtttccat tcggggtctc ctgactgggc
     3181 ttgcccggag cattgtcccc gatatcattc accactctga ccaccgtgcc gtctgcgggg
     3241 gccaataccg gttggccaaa gcagtagaac tgctcgttag tcgtggcgtc tccctcaaaa
     3301 ggaatcccgt tctttagcag aacaaaatcc atggcaaact gttggtcctc gctcatctga
     3361 tacccgttgt taaagacgcc gtgcccgcct tggtacacga gccactggcc cgagaaggga
     3421 agcttcagct tggcggtgtc cttgtagccg gcattgtggc cttcgggagg caaaggttgc
     3481 gctctgaggt tgaaaccctc ggtttcgcca ttctcgtcga tcacgattat ggtcatcact
     3541 ggggccttgg aattcgcgaa ttctgacaag cgggaataaa ccgtagctcg tttcagcatg
     3601 taggggacga tgttctccgc gtctaccttc gtctcagtgc ccagttttgt tttcaactga
     3661 gcgagcgtgg ctacaaattt ccgctcttcc tcggcacgct ttctgttttc cgccggatac
     3721 gtagcccaaa tcgcgctcgc ctggccagct ttgaacattt caaagagctt ccttccccgc
     3781 tccttggccg cctcgctcat tacgggataa gcggcataac tg
//
LOCUS       DATMGX010000511         2671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_142864, whole genome shotgun sequence.
ACCESSION   DATMGX010000511 DATMGX010000000
VERSION     DATMGX010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142864"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1618)
                     /locus_tag="VNR65_08285"
     CDS             complement(113..1618)
                     /locus_tag="VNR65_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18709.1"
                     /translation="MPEGLVERYDERIAGVLSCYDRIVITGTLPGVCHARGMTSFLYA
                     NAIRIFDYPQFAATLRDTVRENAAKIAEAAGVAIEHISKSCVRKEDIVAKAIARRGDH
                     PGLAHVISAMEACGAYEPWHDKKSHKTFVRPDSGKCLHYYFYFIDAELGLIYVRVPTW
                     APFRLQFYCNGHSWLARKLSAEGIGFTAGDNAFLRITDWNRAQELADGFSPVMLHRIL
                     DRYAALCCPVLDTFKQSYHWSLMQVEYSTDLVFHSKQTLAPLYEQLARHSVLTVKAQT
                     IATFLGRSIAPNLAQEIGSQFSTRIEGTCIKHRFGKSSVKMYDKFGIVLRIETTTNDV
                     SSFKHYRKVEHRKGPSSTGFAPMKKNIYSLGDLAGLLFACNRRYLAHLSALDDFSAGV
                     RLLDRVTRPRKVEGKTVKGIDFFNPTERTLLGALQNPRFNIAGVRRADLATLLEHLSP
                     SALSRRIRRLRCLGLIKRVAKTYRYYLTRAGRAVIAAASHITEYGIVPALA"
     gene            complement(1937..>2671)
                     /locus_tag="VNR65_08290"
     CDS             complement(1937..>2671)
                     /locus_tag="VNR65_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18710.1"
                     /translation="ETLEGIVKHNGPLIDHKGAPLALYAEKGLPLAIRATMQVQDLRL
                     DTFASLESQIAALADDIAYNNHDIDDGFRAGYIAVKELEEMPMAGRTLALVRKTHGDL
                     PDSPLIYEMNRRMIAGMVQDVLAETRARITDGRPSTIEDIRGGREPLAQFSEKMAADI
                     ADLRRFLFAKVYKNPAIVRKMTEAQRILRELYDHFAGHPEEMNLEPGREAEIADPIAR
                     QRAVGDFVAGMTDMFALKRHAALFDA"
BASE COUNT          539 a          743 c          836 g          553 t
ORIGIN      
        1 aaggagctgg tggccgagga gaaggcaaag cactagtaga atcggccgcc tccaggaact
       61 ccctatttag tcccttacca accaaatctt gtcactttgc gcacggagtg cgtcaggcga
      121 gtgcgggaac gatgccgtat tcagtgatgt gagaggcggc agcgatgacg gctcttccgg
      181 cgcgggtgag gtagtagcgg taggttttgg caacgcgctt gatgagacca aggcagcgca
      241 ggcggcggat ccggcgcgag agcgccgaag gcgagaggtg ctccagcagc gtggcgagat
      301 cggcgcggcg gacaccagcg atattgaacc tggggttctg taatgcacca agcagggtgc
      361 gttcggtggg gttgaagaaa tcgatcccct tgacggtctt gccctcaacc ttccgcggcc
      421 gggtgacacg gtccagcagt ctgacgccag ctgagaaatc gtcgagcgcc gagagatggg
      481 cgagataacg gcggttgcag gcaaagagaa ggcctgccag gtctccaagg ctgtagatgt
      541 tcttcttcat gggagcgaag cccgtgcttg atgggccttt gcggtgttct accttccggt
      601 agtgcttgaa ggacgagacg tcgttggttg tcgtctcgat acgcaggacg atgccgaact
      661 tgtcgtacat cttgacggag gacttgccga accggtgctt gatgcaggta ccctcgatgc
      721 gggttgagaa ctggctgccg atctcttgag ccagattggg ggcaatactc cggcccagga
      781 atgtggcgat ggtctgcgcc ttcacggtga gcaccgaatg cctcgccagc tgttcgtaaa
      841 gcggcgccag cgtctgcttg gagtggaaca ccagatcggt tgaatactca acctgcatca
      901 ggctccagtg ataggattgt ttgaaagtat cgagcaccgg gcagcataac gccgcgtagc
      961 ggtcgaggat gcggtggagc atgacggggg agaagccatc ggccaattcc tgcgcacggt
     1021 tccaatcggt gatgcgcagg aaagcattgt ccccggcggt gaagccgatg ccttcggcgc
     1081 tcagcttgcg cgccagccag ctgtggccgt tgcaatagaa ttgcaagcgg aacggcgccc
     1141 aggttggaac gcggacatag atcaggccca gttccgcgtc gatgaaatag aaatagtagt
     1201 gcagacactt gccgctgtcc ggccgcacga aggtcttgtg gcttttcttg tcatgccagg
     1261 gctcgtaggc gccgcacgcc tccatggcgg agatgacgtg cgcgaggcct ggatgatcgc
     1321 cgcgccgggc gatcgccttg gcgacgatgt cttccttgcg gacgcagctc ttcgaaatgt
     1381 gctcgatggc gacgcccgcg gcttccgcaa tcttcgccgc gttctcccgg accgtatcgc
     1441 gcagcgttgc cgcaaattgc ggataatcga aaatgcggat ggcattcgca taaagaaacg
     1501 atgtcatgcc cctggcgtgg cagaccccag gaagcgtgcc ggtgatgacg atccggtcgt
     1561 aacaagaaag aacccctgcg atccgctcat cgtagcgttc aacaagcccc tccggcattg
     1621 tcccctcgcc aattgcctgc gcccgccacc actatagcgt aacctctttg gttccggctc
     1681 cgccggctta ggagtgtacg cgcacgtcaa aaacgcccac aaattattct caaaataaag
     1741 aatgacctca aagaagccgg gtccagattc ttgacgagat tgcggcatta cgttctgacc
     1801 tcagaatcgc gcatcgttag tcacgaccgg attgagaccg caagcatggg ttgtcaataa
     1861 ttattggtgc aatcaaaggg tttaatgatt cgggtggcgt aaagccgccg tcgatcgccg
     1921 gtggaactgt tctccttcag gcgtcgaaca gggcggcatg ccgtttcagc gcgaacatgt
     1981 cggtcatccc ggccacgaaa tcgccaaccg cgcgctgcct ggcgataggg tccgcgattt
     2041 ctgcctcgcg cccaggctcc aaattcattt cctcgggatg cccagcgaaa tggtcgtaaa
     2101 gctcgcgaag gatacgctgg gcctcggtca tttttcgaac gatggccggg ttcttgtaga
     2161 ccttcgcgaa gagaaagcgc cgcaggtccg cgatgtcggc ggccatcttt tccgaaaatt
     2221 gagcgagcgg ttcccggcct ccgcgaatgt cttcaatcgt ggatggcctc ccgtcggtga
     2281 ttcgcgctct cgtctcggcc agcacatcct ggaccatgcc cgcgatcatc cgccggttca
     2341 tttcatagat aagaggcgaa tcgggaaggt cgccgtgggt cttgcggaca agagccagcg
     2401 tccggcccgc catcggcatt tcctcaagtt ctttcacggc gatgtagccg gcgcggaagc
     2461 cgtcatcaat gtcatggttg ttgtaggcga tgtcatcggc cagcgctgcg atctggctct
     2521 caagcgaagc aaaggtatcg agtcgcaaat cctggacctg catggtggcg cggatggcga
     2581 gcggcagccc tttttcggcg tagagggcca gtggtgcgcc cttgtggtcg atgagcggtc
     2641 cgttgtgctt gacgatgcct tcaagcgtct c
//
LOCUS       DATMGX010000512         3228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_374274, whole genome shotgun sequence.
ACCESSION   DATMGX010000512 DATMGX010000000
VERSION     DATMGX010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374274"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..323)
                     /locus_tag="VNR65_08295"
     tRNA            complement(248..323)
                     /locus_tag="VNR65_08295"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(288..290),aa:Gly,seq:ccc)
     gene            complement(384..932)
                     /locus_tag="VNR65_08300"
     CDS             complement(384..932)
                     /locus_tag="VNR65_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HWJ18711.1"
                     /translation="MAQFFTIGHSNRPLGEFIRLLGEAGVGLVIDVRTIPKSRHNPQF
                     NAEALEQALPAVGIAYKRIPELGGLRSRRKDQGPSPNGFWENENFRNYADYTATPDFR
                     AGLHKLQALGETRSCAIMCAEAVWWRCHRRIITDYLLSAGESVTHIMGEGKLEPAQLT
                     EAAIIGQDGVITYPAAQGHLLL"
     gene            complement(972..2177)
                     /gene="metZ"
                     /locus_tag="VNR65_08305"
     CDS             complement(972..2177)
                     /gene="metZ"
                     /locus_tag="VNR65_08305"
                     /inference="COORDINATES: protein motif:HMM:NF006003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HWJ18712.1"
                     /translation="MSDTDGIADLHPDTLLVHGGTLRSSFGETSEALFLTSGFVYESA
                     AEAEARFKNEVEGYTYSRFANPTNTMFETRMALLEGAEAARSTATGMAAVSAALLGYL
                     KAGDHVVAAKALFGACRVVIEEMLPRFGISSTLVDGRDLSAWRGAIRDNTRVLFCESP
                     ANPTLELVDLAGVAAVAKSAGALFMVDNVFATPLLQSPLKLGADVVIYSATKHIDGQG
                     RCLGGVVLSNKTFILDHLQNYLRQTGPSLSPFNAWVLLKGLETLPLRVERQTASAAKI
                     ADFLAERPEVERVMYPGRADHPQRALAEKQMKAGGPLVAFHLRADKAATFRFMDALRV
                     IKISNNLGDAKSLIAHPATTTHQRLNDEAKAELGITPGTVRLSVGLEHADDLMADLAQ
                     ALSGTFGRR"
     regulatory      complement(2210..2286)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(2432..2785)
                     /locus_tag="VNR65_08310"
     CDS             complement(2432..2785)
                     /locus_tag="VNR65_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18713.1"
                     /translation="MNTKFVAALAVCATFAVTPALAEEKAPEPKASKAEVQKLLDDIK
                     ADKAKLKQYCDLSNLQDQYEAAAKKADEKKLDAIDKEMEEGAKKMGADFERIMQQLDD
                     ETSALAYDLAKSCGK"
BASE COUNT          634 a          953 c          942 g          699 t
ORIGIN      
        1 cacaacctct cttcgagcct gctgtaccgt tggatcaagt atttccaggc tgatgagccc
       61 gcggccgttg ttaacaatgc gtgttcatgc cgcgacggtg acgcacgttc gcagccgcgg
      121 tcaagcccat ctcgaggtgc ggcaggttga ctcaccccga gcaccgctcc cgttccgtgg
      181 gcgttccgcg aaggcttcct tgcggaacgc ggaccttgca attttgggct taagtcattg
      241 attttattgg agcgggcgac gaggttcgaa ctcgcgaccc tcagcttggg aagctgatgc
      301 tctaccggct gagctacacc cgcgttgcct ttacattatc actggcggcg cggtttgcaa
      361 cagcgcaggc gctctgctca cacttataac aggagatgcc cttgcgctgc gggataggtg
      421 atgacgccat cctgcccgat gatggcggct tcggtcagct gcgcgggctc cagcttaccc
      481 tcgcccatga tgtgggtcac ggattcgcct gccgagagga ggtagtcggt gatgatccgg
      541 cgatggcagc gccaccacac cgcctcggcg cacatgatgg cacagctgcg cgtttcgccg
      601 agcgcctgaa gtttgtgaag gccggccctg aagtccggcg ttgcggtgta atcggcgtag
      661 ttgcggaaat tctcgttttc ccaaaagccg ttgggagacg ggccttgatc cttccgcctg
      721 ctccgcagac ctccgagttc gggtattcgc ttgtacgcaa ttccgaccgc tggcaacgcc
      781 tgctccagcg cctccgcatt gaattgcgga ttgtgccgcg atttcgggat ggtgcgcacg
      841 tcgatgacga ggcccacgcc tgcttccccc agaagccgga tgaactcgcc caagggccgg
      901 ttcgaatggc cgatcgtgaa aaactgggcc aaggccgctc ccaaggtcct gtcgatttgc
      961 cgttatagac tttaccgacg cccgaatgtt cctgaaagcg cttgcgccag gtccgccatc
     1021 aggtcgtccg cgtgttccaa tcctaccgag agacgtaccg tgcccggcgt aatccccaac
     1081 tccgccttcg cctcgtcatt gagccgctga tgcgtggtgg tcgccggatg cgcgatgagg
     1141 ctttttgcat cgccaagatt gttcgagatt ttgataacgc gcagcgcatc catgaagcgg
     1201 aatgtcgccg ccttgtccgc cctaaggtga aaggcgacca agggtccgcc tgccttcatc
     1261 tgcttctcgg ctaaggctcg ttgaggatga tcggcgcgcc cgggatacat gacacgttcg
     1321 acctcgggcc gttccgccag aaaatccgcg atcttggcgg cgctcgccgt ctgccgctcg
     1381 acgcgaaggg gaagggtttc gagccctttc aaaagcaccc aggcgttgaa ggggcttagc
     1441 gaagggccgg tctgcctcag atagttctga agatggtcca ggatgaacgt cttgttcgac
     1501 agcaccacac cgccaagaca gcggccctgc ccgtcgatgt gtttcgttgc ggaatagatc
     1561 accacgtccg ctccaagctt cagcgggctt tgcagcaagg gcgtggcgaa cacattgtcg
     1621 accataaaga gcgcgccggc gcttttcgcg accgcagcca cccccgcgag gtcgacaagc
     1681 tccagggtgg ggttcgccgg gctttcgcag aaaagcacgc gggtgttgtc gcggatagct
     1741 cctcgccaag cggagagatc gcggccgtca acaagagtgg atgagatgcc gaaacggggc
     1801 aacatttcct cgattacgac gcggcacgcg ccgaagagcg ctttagccgc gacaacgtga
     1861 tcgcccgcct tcaaataacc cagaagcgcc gcagacaccg ccgccatgcc tgtcgccgtc
     1921 gagcgcgcgg cctccgcgcc ttcgagtaag gccatccgcg tctcaaacat ggtgttcgtc
     1981 gggtttgcga aacggctata ggtgtaaccc tccacctcgt tcttgaagcg cgcttcagcc
     2041 tcggctgcgc tttcgtaaac gaagccggat gtcagaaaca gcgcctcgga cgtttcgcca
     2101 aaggaagagc gaagcgtgcc gccatgaacg agaagcgtat cgggatgaag gtctgcgatg
     2161 ccgtcggtat ctgacattgt ccctccttaa gggcgataaa aaaagccgcg ccggagagct
     2221 gacggccttt tagcgagttg tttatcgtgg ctgcaagccg gccggcaaag gaccacgtgg
     2281 catcataaaa acgccattgc gagacgcgcg tcaagcgtcc tcagcatccc tggaataagt
     2341 taaaatatct gccagtaacc agccagacca gcaattcgcg gagaaattcg ccggacgacc
     2401 ggcggtcctt tcgcgttcga agctttggaa gctacttgcc gcagcttttg gcgagatcat
     2461 aggcgagcgc gcttgtttca tcgtcgagtt gctgcatgat cctttcgaag tccgcgccca
     2521 tcttcttagc gccttcttcc atctccttgt cgatagcgtc gagcttcttt tcatccgcct
     2581 tcttggccgc ggcttcgtat tggtcctgaa ggttcgagag atcgcagtac tgtttcaatt
     2641 tcgccttgtc ggccttgatg tcatccagaa gcttttgcac ctcggccttg ctcgccttgg
     2701 gctcgggcgc tttctcctca gcgagtgcag gagtgactgc gaaggtggcg caaaccgcga
     2761 gggctgccac aaatttggtg ttcatgaaag gctcctggac ctgatgaagg gtagcggtaa
     2821 aaggtctatc gaaatcgcag tctgaccgga aacttgcatt tggaccgtcc acaagtttct
     2881 aaaaacggcg ttttgagtag aagaattgtt ctctgtagtg cttggcatag ttttggaggt
     2941 cgcacctgtg tatctagtat tgatatgcat aatacttgct aatctatccc tattgaaacg
     3001 acgggagggg tttgtcccca gaaacagacg ccccttccgt ccaacggagc cggttaccgc
     3061 gccaaggaag tgcgggtgga acttacgccg caagcgaagc cggacgcgcc cacgagagag
     3121 gcctcgcaca agcccatttc gcgacggtgt tgatgtcgta gaagcggcag gcttccatca
     3181 gcatcctgca ttcggtccag atgtcgccgt ccaccatcgg catgaaga
//
LOCUS       DATMGX010000513         2141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_416311, whole genome shotgun sequence.
ACCESSION   DATMGX010000513 DATMGX010000000
VERSION     DATMGX010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416311"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1992
                     /locus_tag="VNR65_08315"
     CDS             229..1992
                     /locus_tag="VNR65_08315"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWJ18714.1"
                     /translation="MTKPIDTGRRLVAIFAADVEGYSRLMGADEVRTLRDLTERRGIL
                     DGLIASHRGRIANTAGDSVLAEFSSAVDAVQCAVEAQIALAAANVGFSPDQHINFRIG
                     VHVGDVMVKGGDLFGDGVNIAARLQTVATAGGVCISAVAHEQVRKILQLNFKDLGTQS
                     LKNIEEAIRAYAVSAADAGALGTSIILSPPKPPERPSIAVLPFANMSGDIEQEYFADG
                     ISEDIITALSKLSKLFVIARNSSFAFKGKNVHVQEVSTKLGVRYVLEGSVRKSGNRVR
                     ITSQLIDATTGGHLWGERFDRDLTDIFAVQDDVTHQIVAALSVNLSPGDRQRLSAEHT
                     ENLEAFDYFLRGRELWWLHAKETNFKARELLQHAIELDPNFAPAHGFLAAAHINDYVS
                     QWSALPSQSLEKAHEAVMRAAALDDKYPYTYWGMTFVNLWMRRYDDAVRAAERSISLN
                     PNFAEGHNALGVILHYVGRSEEAFGCFDRAMALDPLCPGMWLHFQAQAAFQLGRYETA
                     VALLKRRIVRNPDTDASRVLLAASYGQMGRIEEARAEWQEVFRVNPDYSLEHRRRVLP
                     YKNPDDFECVVDGLRKAGLVA"
BASE COUNT          440 a          597 c          636 g          468 t
ORIGIN      
        1 gatcgagtag gcgggccgac ttccggcttt ggcacctttg agacatgccg actgaccctt
       61 ggaatgtcgg ctaatcgggg aagaccggaa gtaagcggcg cacggtcaaa gtgacgcgat
      121 gaccacgaac ggacatttga cgctttgccc gatgagaggt cgttccaatt ttggggtgtc
      181 ccgccacttg gtcccgcgat aggctgttag cggatttagg gggcgtattt gacaaaacca
      241 attgataccg ggcgcaggtt ggtcgcgatt tttgcggctg acgtcgaggg ttatagtcgc
      301 ttgatgggag ctgacgaggt ccggacgctg cgggacctga ccgagcgccg tggcatcctc
      361 gacggcttga tcgcatctca ccgcggccga atagcgaata ccgctggtga cagtgtgctg
      421 gcggagtttt caagtgcggt cgatgctgtg caatgtgcgg tcgaggcgca gatcgccctt
      481 gccgccgcta acgtcgggtt ttcgcccgat caacacatca atttccgcat cggtgtccac
      541 gttggagacg tgatggtgaa gggcggggac ttgttcgggg acggagtcaa tatcgctgcc
      601 cgcctacaaa cagttgcgac tgcaggcggc gtttgcatct ccgcggtagc tcacgaacaa
      661 gtcaggaaaa tcctacagct caatttcaag gatctcggca cccaatctct aaagaatatc
      721 gaagaggcga ttcgcgccta tgccgtcagt gcggccgacg caggggcctt gggcacctca
      781 ataatcttga gcccgccgaa gccgcctgaa agaccctcca tcgccgtgtt gcccttcgcc
      841 aatatgagtg gcgacatcga gcaggaatat ttcgccgatg gaatttccga agatatcatc
      901 accgccttgt cgaaactatc gaagctcttc gtcatcgctc gtaattcctc cttcgccttc
      961 aaggggaaga atgtccacgt gcaggaggtg agcacgaaac ttggcgttcg ctatgtcctg
     1021 gagggaagcg tccgcaaatc cggcaaccgg gttcgaatta catcccagct catcgatgcg
     1081 accaccggtg gacatctctg gggcgagcgc ttcgatcggg acttgacgga cattttcgcc
     1141 gttcaagacg acgtgacgca tcagatcgtc gctgcgctct cggtcaacct ctcgccgggc
     1201 gatcggcaac ggctctcagc agaacatact gaaaatctcg aggcttttga ctatttcctg
     1261 cgcggccgcg aactctggtg gcttcacgca aaggagacca acttcaaggc acgcgaatta
     1321 ctacaacatg cgatcgaact ggatcccaac tttgctcccg cccatgggtt tctcgccgcg
     1381 gcccatatca acgactacgt cagtcaatgg agcgcgttgc cttcgcagtc gctcgaaaaa
     1441 gcacatgagg cggtgatgcg ggctgcagcc ctcgacgaca aataccctta tacttattgg
     1501 gggatgacct ttgtcaattt gtggatgcgt cgctacgacg acgccgttcg cgctgccgaa
     1561 agatcaatct cgctcaatcc gaactttgct gaaggtcata atgccctcgg tgtgattttg
     1621 cattatgtcg gccggtccga ggaggctttc ggatgcttcg accgcgcgat ggccctggat
     1681 ccgctttgtc ctggcatgtg gctgcacttt caggcgcagg cggcctttca actgggccga
     1741 tacgaaacgg ctgtagctct gctgaagcgg cgaattgtcc gcaacccaga taccgatgcc
     1801 tcgcgcgtct tgctggcggc gagctatggt cagatggggc gtattgaaga ggctcgcgcg
     1861 gagtggcagg aggtattccg cgtcaacccc gactattcat tggaacaccg tcgcagggtg
     1921 ctgccgtaca aaaatcccga cgatttcgag tgcgttgtgg acggtctccg caaggcgggc
     1981 ctagtcgcgt aatgcgcgcc gggatggcta cttccgggtt tggcacatag cgtcagttat
     2041 tgcggtgcag cgccatgtcc gcagttgggc gcaaaccaga agtgatccgc acatggccaa
     2101 agtgacgcga ttggcccatc agacgtcggg atgctctccg a
//
LOCUS       DATMGX010000514         6027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_170037, whole genome shotgun sequence.
ACCESSION   DATMGX010000514 DATMGX010000000
VERSION     DATMGX010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170037"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VNR65_08320"
     CDS             complement(<1..524)
                     /locus_tag="VNR65_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18715.1"
                     /translation="MAQDGSFVANGSVEPGPVAPPKVDLIYNPFELRALRSKFLTSGT
                     WLIALLGAIPLLSVIYMLVVKGGARLGYELFTELPPAGFELGGGIGNAIQGTLVTVGI
                     AALISIPIALLAAIYLAELRPNSQISVWARFLSKVLTGFPSILAGVFVYAAVVLLMGT
                     YSALAGGIALAVLML"
     gene            complement(528..1502)
                     /gene="pstC"
                     /locus_tag="VNR65_08325"
     CDS             complement(528..1502)
                     /gene="pstC"
                     /locus_tag="VNR65_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWJ18716.1"
                     /translation="MSSVSFELPESSRSLARPPSITAYFVDRLFFAIATIGALAIIAL
                     VAYILYEIGAQAFPAIQRYGASFLVDTQWDVQKSHFGILPQIWGTLYSSLLALMIGGF
                     VGVTIAIFLTQDFLPPRLAVSFRTLIEMLAAIPSVVFGLWGIFVVIPFIRPAANWLNE
                     YFSYIPFFSSSLSGPGLLPAVIVLSIMILPTVAAISQDAIGLVPYRVKEAAYGMGTTK
                     WEAILKVILPTASAGISSALVLGFGRALGETMALAMLIGNSNQISLSLFSPANTLAAL
                     LASNFPEAAQTERQALMYAALVLLAITLIVNIIGTLILAYTSRKMGKA"
     gene            complement(1542..2561)
                     /gene="pstS"
                     /locus_tag="VNR65_08330"
     CDS             complement(1542..2561)
                     /gene="pstS"
                     /locus_tag="VNR65_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWJ18717.1"
                     /translation="MKLKSILTTAAAFALTLQVSQAADMRLVGSGASFPFPIYSAWFK
                     DFSRKTDGVSVDYQAKGSGAGIQDLINKTVDFAASDAAMTPEQIAQVKDGVVLLPMTA
                     GEIVLAYNLPGQPKNLKLPRDVYPDIFLGKITKWNDPRIAAANPDIKLPDLPITVVRR
                     SDASGTNFVFTKHLSAISPAFKEKVGFGTTVSWPQSDKLVAAPKNDGVTATIKQTPGA
                     IGYIEYGYAKLTKADTALLQNAAGNYIAAGDASGAAALANAQLPADLIAWVEDPAGSD
                     SYPIVSFTWMLFYEKQDAQKAENLRKLVAYGLSDGQKIADSMGYIPLPSSVVEKVKAA
                     SEKIQ"
     gene            2953..5328
                     /locus_tag="VNR65_08335"
     CDS             2953..5328
                     /locus_tag="VNR65_08335"
                     /inference="COORDINATES: protein motif:HMM:NF022783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HWJ18718.1"
                     /translation="MEPTFSPEKAQARMANDASTIAELVQRLSFCYSRHVERMSRDHG
                     ERLKSFLKDGASLGGRFADSAKDRKIFEDANAYLTDAARRYFLTLDTLRERGNNDIEH
                     EAAGTPPVLVYKTEVVVDGRTLPRPVNYVLLKIIPPEGVQVYDWKRPYMIIDPRAGHG
                     AGIGGFKQDSQVGVALHDGHPVYFVVFRPHPEPGQTLADVMRAEAEFVSEIVRRHPKA
                     AKPIIVGNCQGGWAAMILAAANPDITGPLVINGAPMSYWSGRIGENAMRYNGGLLGGI
                     LPALLLADMGHGEFDGAHLVSNFEMLNPGRNYFGKYYDLFANPERGREKFLEFERWWG
                     GFHFMNEVEIRWIIEQLFVGNRLSRGEARIEHGRQLDLKSIRSPIIVFASWGDNITPP
                     QQALNWIADTYADEQEIKIRGQRIIYMVHEKVGHLGIFVSSSIAKKEHAEVTSTMKTI
                     EALSPGLYEMMIEEQIGEGVHAHFRVSFQERKVSDLLVKIDDNDRSEEKDFAAVARLS
                     ELGGNFYEMALRPFVQAAITPQAAKAMRELHPARSNRLMFSDRNPLMRSVSDTAKRLE
                     SNAKPLNGANPFLEVERLWAESVIQGFDVLRDMRDTLYEMTFLTIYGSPFMRWLGAPY
                     AFERTRKDPKELRFLPEVQAILLGIDRGGFEEAVIRMLILLAEARGSVRRDRLERSSK
                     VLTRDEPFASLGVERRAALIREQSIIAEFEPDRAIETLPNLLPDLEERRKAMQVVEYI
                     AGSMEEMEPHTLKTLQRFHAVLSLPLITSSLIAHDPLQESPDRQGALEAAE"
     gene            5474..>6027
                     /locus_tag="VNR65_08340"
     CDS             5474..>6027
                     /locus_tag="VNR65_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18719.1"
                     /translation="MSNENTKSPSDIQRRVNAEMIDSFDEELEMELDDDRVSAMLEPT
                     QDYQSPESMDRHLYFRELFRLQRELVKLQDWVQHKKLKLVVIFEGRDSAGKGGVIKRI
                     TQRLNPRVCRVAALPAPSQRERTEWYFQRYVQHLPAGGEIVLFDRSWYNRAGVERVMG
                     FCTEEEVEEFFHSVPEFEKMLVRSG"
BASE COUNT         1303 a         1722 c         1790 g         1212 t
ORIGIN      
        1 agcatcagga cggcaagcgc gatgccccca gccagcgcgg agtatgtgcc catcagcaac
       61 acgaccgcgg cgtagacgaa cacgccggcg aggatcgatg ggaagcccgt gaggaccttc
      121 gacaaaaatc gtgcccagac cgaaatctgg ctattggggc gcagctccgc gaggtagatg
      181 gcggctaaga gtgcgatggg gatgctgatg agcgctgcaa taccaaccgt caccagagtg
      241 ccttggatcg cattgccgat gcctccgccg agttcgaagc cggcaggcgg cagctctgtg
      301 aagagctcgt agcccaatcg agcgccgcct ttcacgacca acatgtaaat cactgaaagg
      361 aggggtatcg caccaagaag agcgattagc caagtgccgg acgtcagaaa cttgcttctc
      421 agcgcacgca actcgaaggg gttgtatatg aggtcaacct tcggaggcgc aaccggaccg
      481 ggctcaactg atccattggc gacgaatgag ccgtcttgag ccatgggcta tgccttcccc
      541 attttcctgg atgtgtaggc caagatcagc gtccctatga tgttgacgat gagcgtgatc
      601 gcgagcagga cgagcgcggc atacatcaga gcctggcgtt cggtctgggc ggcttccgga
      661 aagttggatg ccagcagcgc cgccagcgtg ttcgccggag agaagagcga gagagagatt
      721 tggttggagt tgccaatcag catggccagc gccatagtct ctcccagggc acggccaaat
      781 cccaaaacga gcgcgcttga aatgccggcc gaagcagtcg gcagaatgac cttcaggatc
      841 gcctcccact tggtggtccc catgccgtag gctgcttcct tgaccctgta gggaaccaaa
      901 ccgatggcgt cctgcgaaat tgccgcgacc gtcgggagaa tcatgatcga aagaacgatc
      961 actgccggca gcaatcctgg cccgctcagt gaggatgaga aaaagggtat gtaactgaaa
     1021 tactcattga gccaattggc ggccggacgg atgaacggga tgacgacgaa aataccccaa
     1081 agcccgaaga cgacgctcgg gatggccgcc agcatctcga tcagagtgcg gaagctgacc
     1141 gccagacgcg gcggcaggaa gtcctgcgtc aggaagattg cgatcgtgac gccgacaaaa
     1201 ccgccgatca tcaaagccag tagcgaactg tagagggtgc cccagatctg cggcaggatc
     1261 ccgaaatgtg atttctgcac atcccattgc gtatcgacca ggaagctcgc gccgtagcgc
     1321 tgaatcgccg ggaaggcttg agccccgatc tcatagagga tgtaggccac caaagcgatg
     1381 atggccaacg cgcctatggt tgcaatcgcg aagaagagcc ggtcgacgaa ataggctgtg
     1441 atagagggcg gccgcgccag cgaccggcta ctttctggca actcgaacga aactgacgac
     1501 atggaaacct gctggcgcgc gattgcgggc gtcggaaggg actactgaat cttttccgac
     1561 gcggctttca ctttttctac gacgcttgac ggcagcggga tgtagcccat gctgtccgca
     1621 atcttctggc catcgctcag cccataggcg acaagcttgc gaagattctc agccttctgc
     1681 gcatcctgct tctcgtagaa gagcatccag gtgaagctga cgatcggata ggagtccgat
     1741 ccggcagggt cctccaccca ggcgatcaag tccgcgggca gttgagcgtt ggcgagcgct
     1801 gctgcgccgg atgcgtctcc cgccgcaata taattgccag cagcgttctg caacaaggca
     1861 gtatcggcct tggtcaattt cgcgtagcca tattcgatat agccgatggc gcctggggtt
     1921 tgcttgatcg ttgccgtcac gccatcattt ttcggcgcgg cgacgagctt gtcgctttgc
     1981 ggccaggaca ctgtcgtccc gaagcccacc ttctccttga acgccgggct gatcgccgaa
     2041 agatgcttcg tgaacacaaa attcgttccg ctggcatccg agcggcgaac gacggtgatc
     2101 ggcaggtcgg gcagctttat gtccggattt gccgccgcaa tgcgtggatc gttccacttc
     2161 gtgatcttgc cgaggaaaat gtcagggtag acgtcgcgcg gcagcttcag gttcttcggc
     2221 tgaccgggaa ggttataggc cagcacgatt tcgccggccg tcattgggag gaggacaacg
     2281 ccatccttca cttgtgcgat ctgttccggc gtcatggccg catcgctcgc ggcgaagtcc
     2341 actgtcttgt taatgagatc ctgaatgccg gcgccgcttc ctttggcttg gtaatcgacg
     2401 ctcacgccat ccgtcttgcg gctaaaatcc ttgaaccaag ccgagtaaat cggaaaaggg
     2461 aagctggccc ccgatccgac gagacgcata tcagctgctt gggagacttg taaggttaag
     2521 gcaaaggctg ctgcggttgt caggatcgat ttcaacttca ttgcaaacgc tccacggacc
     2581 cggaattact ttgttttttc tcaccgctgg gcgcagcgag ccatgccgag agcaaggctg
     2641 ctacagctaa gaatacttaa tacaagttac atgacacatg gatgacagct tcgccttggc
     2701 ggcagcgcta cccgagagct cggcgtcatt ttcctgttac aagccagcta tagcctcagc
     2761 ttcgagggat tatgggctcg ctaagctgtc ccaaggtgca ctgcacgcgt agaagcactt
     2821 tggagacgag gcacttagcg cagtgacttc ttgggccggc agcagagatg cctggcattt
     2881 cccccgggta agcttctggc taaagagggc gaaccacccg gccatatcta gcaatcaaat
     2941 acgaggtctg cgatggagcc gacatttagc cccgaaaaag cgcaagcgcg catggcgaac
     3001 gatgcctcca ccatcgcgga gctggtacag cgcctgtcct tttgttactc gcgccatgtc
     3061 gagaggatga gccgggacca cggcgaaagg ctgaagtctt ttctcaaaga cggggcatcg
     3121 ctcggcggcc gctttgctga ctccgcgaaa gatcgtaaga tattcgaaga cgccaacgct
     3181 tatctgacgg acgccgcgcg ccgttacttc ctgacgctcg atacgcttcg tgagcgcggc
     3241 aataacgaca tcgaacatga ggcggctggc acgccgccgg tgcttgtcta taaaactgaa
     3301 gtcgtggtcg atgggcgcac actgccccgt ccggtcaatt atgtgcttct caagatcatt
     3361 ccgccggaag gggtccaggt ttacgactgg aagcgcccct atatgattat tgatccgcgt
     3421 gcgggccacg gcgctggcat cggcggcttc aagcaagaca gtcaggttgg cgtcgcgcta
     3481 catgatggac atccggtcta tttcgtcgtc ttccgccccc atcccgagcc gggtcagacg
     3541 ctggccgacg tcatgcgcgc cgaagccgaa tttgtgagcg agatcgttcg ccgccacccg
     3601 aaagcggcaa agccaatcat cgtcgggaac tgtcagggcg gctgggccgc catgatcctt
     3661 gcggcggcca atccggacat taccgggccg ctcgtaatca atggcgcgcc catgtcctat
     3721 tggtcgggtc gcattggcga aaacgcgatg cgctacaatg gcggactgct tggcggtatc
     3781 ttgcccgcgc tcttgttggc cgatatggga catggcgaat tcgacggcgc ccatctcgtc
     3841 tccaactttg agatgctcaa tccgggccgt aactatttcg gcaagtatta tgacctgttc
     3901 gccaatcctg agcgcggccg cgagaagttc ctcgaattcg agcgctggtg gggcggcttc
     3961 cacttcatga acgaagtcga gatccgctgg atcatcgagc agctcttcgt cgggaaccgg
     4021 ctgtcgcgcg gcgaggcccg catcgagcac ggccgtcaac tcgatctgaa gtcgatacgc
     4081 tctccgatta tcgtcttcgc aagctggggc gacaacatca cgccaccgca gcaagcgctc
     4141 aactggatcg ccgatactta cgcggacgaa caggagatca agatccgcgg ccagcgcatc
     4201 atttacatgg tgcatgagaa agtcggccat cttggcatct tcgtatcctc ttccatcgcc
     4261 aagaaagaac acgccgaagt cacctcaacc atgaagacga tcgaggcctt gtcgcctggc
     4321 ctctacgaaa tgatgattga ggaacagatc ggcgaagggg ttcacgccca cttccgggtc
     4381 agcttccagg aacgcaaggt gtcggatctg ctcgtcaaga tcgacgacaa tgatcgttcc
     4441 gaggaaaagg attttgccgc cgtagcccgc ctgtcagaac ttggtggcaa cttctatgaa
     4501 atggctctcc ggccctttgt tcaggctgcg attacgcctc aagccgcgaa ggccatgcgc
     4561 gagcttcacc cggctcgctc caatcgcctc atgttctccg accgcaatcc cttgatgcgg
     4621 tctgtgtctg atacggcgaa gcgccttgaa agcaacgcaa agcccctcaa cggcgcaaat
     4681 ccatttcttg aagtggagcg gctctgggct gaatcggtaa tccagggctt cgatgtcctg
     4741 cgcgacatgc gcgacacgct gtatgagatg acgtttctca cgatctacgg gtcgccattc
     4801 atgcgctggc tgggcgcccc ctatgccttc gaacggacgc gcaaggatcc gaaagagctg
     4861 cgcttcctgc cggaggttca ggctatcctt ctcggcatcg accgcggcgg cttcgaggag
     4921 gcggtcatcc gcatgctcat cctgcttgcg gaggcgcgcg gctcagtccg ccgtgatcgc
     4981 cttgaaagat caagcaaagt gctgacgagg gacgagcctt ttgcgtcgct cggtgtggag
     5041 cggcgggccg ctctcatccg cgagcagtcg atcattgccg aatttgaacc agaccgtgcg
     5101 atcgagactt taccgaatct tctgcctgat ttggaagaac gccggaaggc gatgcaagtg
     5161 gttgagtata ttgcggggtc catggaggaa atggagccgc acacgctgaa gactctgcaa
     5221 aggtttcatg cggtcctcag cctgcctctc attacgtcga gcttgatcgc tcacgatccg
     5281 cttcaagaga gccccgatcg ccaaggagcg ctggaggcag cggagtaacc gctgccatcc
     5341 acctggttta ctgaggcgtg agagagcatg ctgcgggaaa caatcctttg aactctgagg
     5401 cgtttacctt ctcaggctct tacgactatt atcttcccaa ttcatcgttg tgacgttgga
     5461 caggatcgcg tgcatgagta acgagaatac gaaatctcca agtgacatcc agcgccgcgt
     5521 gaacgccgag atgatcgaca gcttcgacga ggagcttgag atggaactcg acgacgatcg
     5581 cgtgtccgcg atgcttgagc cgacccagga ctatcagagc cctgaatcta tggatcggca
     5641 cctttatttc agggagctgt tccgccttca gcgcgagttg gtgaaactac aggactgggt
     5701 gcagcacaaa aagctgaagc ttgtggtcat ttttgaaggg cgcgactcgg ccggcaaggg
     5761 cggcgtcatc aagcgcatca cgcaaaggct caatcctcgc gtatgcaggg tcgccgcgtt
     5821 gccagcgccg agtcagcggg agcgcacgga gtggtacttc cagcgctatg tgcagcacct
     5881 gcccgcaggc ggcgagatcg tgctcttcga ccggagctgg tacaatcgcg cgggcgtcga
     5941 gcgcgtcatg ggcttctgca ccgaagagga ggtggaggaa ttcttccaca gcgtgcccga
     6001 attcgagaag atgctggtgc gctcggg
//
LOCUS       DATMGX010000515         4372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_447254, whole genome shotgun sequence.
ACCESSION   DATMGX010000515 DATMGX010000000
VERSION     DATMGX010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447254"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2173)
                     /locus_tag="VNR65_08345"
     CDS             complement(<1..2173)
                     /locus_tag="VNR65_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWJ18720.1"
                     /translation="MSSVRLLTIVRGFLVALLCCTAAFASDFKVSVESPAGGRVSGVQ
                     VSLFRIGDNAGVGVQTTAGDGTATFPHLAAGQYRLVVLAPGFAEQSQQISIPATESLN
                     VQLTLTTTPQTVVVSATATPATAEQTGTSVGVLTSEQLTAINPVAATDALLYIPGAIV
                     SSTGRRGNLASLFVRGGESNYNKVLVDGVPVNDPGGIFDFGVVPMNNIDRLEVERGAE
                     STVYGSDAMTSVVQLWTATGSTRTPEIQFGADGGNFSTANGYASLAGARGIFDYNVFA
                     YQFNTQGQGVNDAYSNSLQGGNVGVRLSDRVAFRLRARHYNSWTGIQSNWWFNGNPEL
                     PPDPNQYAHQNNFLASGDLTISGPGAWQHQFSGFEYHHVALNVNPLDDLDRPFDSAFN
                     SLAKYNLAGFAYQEIYSPRPWAQTTLGYNFQDENGYINNNSDFGISTTHGLRFNNYLF
                     AQQTIVWRRLSVLAGVGYVNNSSFGGKVSPRASLTFLALRGNTVFSGTRLHFGYSEGI
                     KEPSFEQTFGITGTFPTLPNPNLQPEQNRAIETGVLQSLFNNRVSLSAVYFHNQFRDQ
                     IEYVYNSDTNTSQYVNFNRAMAQGAEVVLSGRVANHLSATAAYTYTSTQIQQAPPCDP
                     NAGCDPRIYGTGAPLLRRPKQAGMLLLTYTRQRWGGFVGVTAVGQRPDSDFQFGYIPP
                     IYYAAGYARLDFGGGYAVNHHITAYANVNNALNNHYNEVLGY"
     regulatory      complement(2246..2440)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2506..3219)
                     /locus_tag="VNR65_08350"
     CDS             complement(2506..3219)
                     /locus_tag="VNR65_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWJ18721.1"
                     /translation="MPNPTSAAAGEIHQFRLTLLQRMQLTLVSWIASAVVGLIGRTLR
                     MTITYEEGSIRGLDSIAPGIYPFWHRCVLPATWLFRNRDIGVLTSLSRDGEYIARVIR
                     RFGFVPVRGSSSRGGQRGLLEMETMVKAGGAVAFTIDGPRGPRYVAKKGPVTLARLTG
                     IPITAFYVAVEKAWVLKTWDAMLIPKPFSRAYVRVAKNIFVPPDADDAALDRYHAEMQ
                     ASLERITGFAESQFSARHD"
     gene            complement(3229..>4372)
                     /locus_tag="VNR65_08355"
     CDS             complement(3229..>4372)
                     /locus_tag="VNR65_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ18722.1"
                     /translation="GGARRIVIVAGNPEIATAVEQQLLETESTPGATSQADRQATESY
                     AVGISTTPTEAERNDLLQQVTDGKIDGFLWLTDEAVAQHKVTYLTRDAADFRGNRELR
                     SAVTAALAKHRLAEKGMSGPEVEALLTPITLDAVHIEKGKEGVSGAAIFATAVAMVML
                     LYAVVLIYGVAVMRSVIEEKSSRIIEVLLSSVTAKELLAGKILGVGAVGLTQILIWII
                     FAVTFAVSGLMAAKSFVSEIHIPTAGVIFFGVFFVLGYFLYSAMYAAIGSMVTTDQEA
                     QQMQWPAMVPIILAVFLMNPVIQQPNSQLAFWLSIIPFFAPILMLVRILVEEPPVWQL
                     ALCLGLMLATIYGLLSLASRIYRVGILMYGKRPTLPELRRWLRYTG"
BASE COUNT          883 a         1273 c         1365 g          851 t
ORIGIN      
        1 gatatcccag cacttcgttg tagtgattgt tcagcgcgtt attcacgttc gcgtacgccg
       61 tgatgtgatg attgacggcg tacccgccgc cgaaatcgag gcgcgcataa cccgccgcat
      121 agtagatggg cggaatgtat ccgaattgga aatcagagtc cggacgctgc cccactgcgg
      181 tgacaccaac gaatccgccc caacgctgcc gcgtataggt cagcagcagc atgcccgctt
      241 gcttgggacg ccgcagcagc ggagctcccg tgccgtagat gcgcggatcg caacccgcat
      301 tgggatcgca gggcggcgct tgctgaatct gagttgaagt gtaggtgtaa gcggcggtcg
      361 ccgacagatg gttggcaaca cggccgctca acaccacctc ggcgccctgc gccatcgcac
      421 gattaaagtt gacgtattga ctggtgttgg tatcggagtt gtacacgtac tcgatctggt
      481 cgcgaaactg attgtggaaa tacacggcgc tgagtgacac gcggttgttg aacagcgact
      541 gcagcacgcc ggtttcgatg gcccggttct gttccggttg caggttggga ttcggcagcg
      601 tgggaaacgt accggtgatg ccaaaggttt gctcgaagct gggctccttg attccctcgg
      661 agtagccaaa gtgcaggcgc gtgccactga agacggtgtt tccgcgcagc gccaggaacg
      721 tcagggaagc gcgcgggctg actttgccgc caaaactgct gttgttgacg tagccaacgc
      781 ctgccagaac cgacagccgc cgccacacga tggtctgctg tgcaaacaga tagttgttga
      841 agcgcaaacc gtgagtagtg ctgattccaa agtccgaatt gttgttgatg tatccgttct
      901 cgtcttgaaa gttgtaaccc agcgtggttt gcgcccacgg ccgcgggctg tagatttcct
      961 gataagcgaa accggcgagg ttgtacttgg ccaggctgtt gaaggcgctg tcgaatggcc
     1021 ggtcaagatc gtcgagggga ttcacgttaa gcgcgacgtg gtggtactcg aagccgctga
     1081 actggtgctg ccacgcgccc ggcccgctga tcgtaagatc gccgctggcc aggaaattgt
     1141 tctggtgcgc gtattggttc gggtccggcg gcagctcggg attcccgttg aaccaccagt
     1201 tcgactgaat gcctgtccaa ctgttgtagt gacgtgcgcg caggcggaat gccacgcgat
     1261 cggaaagtcg cacgcccaca tttccgcctt gcagcgagtt cgaataggcg tcgttcactc
     1321 cctggccttg cgtgttgaac tggtaggcga agacgttgta gtcgaaaatt ccgcgcgctc
     1381 ccgcgagcga cgcatagccg ttggcggtgg agaaatttcc gccgtcggcg ccgaattgaa
     1441 tctccggcgt gcgcgtgctg ccggtcgcgg tccaaagctg caccacgctg gtcatggcgt
     1501 ccgatccgta gacggtgctt tcggcgccgc gttccacctc gagccggtca atgttgttca
     1561 tgggaaccac gccgaaatcg aagatgccgc cgggatcgtt cacgggcacg ccgtccacca
     1621 gtaccttgtt gtaattggat tccccgccgc gaacgaacaa gctggcgaga ttgccgcgcc
     1681 gcccggtgct gctgacgatg gctccgggaa tatagagcaa ggcatccgtg gcggcgacgg
     1741 gattgatggc cgtcagttgc tcactggtta gcaccccgac cgaagtgccc gtctgctccg
     1801 ccgtagcggg agttgcggtg gcggagacga ccacggtttg cggcgtggtg gtgagcgtca
     1861 gttgaacgtt gaggctttcc gtcgccggaa tggaaatctg ttgcgactgc tcggcgaatc
     1921 ccggtgcgag cactaccagc cgatactgcc cggcggcgag atgcggaaac gttgctgtac
     1981 catcgccggc tgtggtttgc actcctacgc cggcgttgtc gccgatgcga aacaagctga
     2041 cctgcacacc ggagacgcgc ccgccggcag ggctttccac tgacactttg aaatcggatg
     2101 cgaaggctgc tgtgcagcac agcagtgcga cgagaaatcc acgaacaatg gtcaagagac
     2161 gaacgctgga catgacactc ttcctttaca cgcgaaaggg gttggtgatc agggcacccc
     2221 catcgcacgc aaagtcggcg tgcgccgggg cccccgtagt tcgcgccggt ctcctggctt
     2281 gcgagttgcg atttgcatcg cggacgacgg cgggggcctt cccgtttgca cagtggccga
     2341 tccaccaaag actcgcttac agttgcgcgg cagcgcgggc ttcacacccg cttcccagtt
     2401 ccgccggtga gatgacggaa cgcgcgaaca cgaattggca aggaacaatc ccggaccaag
     2461 ttggttcagg atgagattcg actgtactga gagtgaaagc gggtgtcaat cgtgacgtgc
     2521 ggaaaactgc gattccgcga agccggtgat gcgctccagc gaggcttgca tctcggcgtg
     2581 atagcgatcc agcgccgcat catcggcatc gggcggcacg aagatgtttt tcgccacccg
     2641 cacataggcg cgcgaaaacg gtttgggaat gagcatggcg tcccaggtct tcaagaccca
     2701 ggccttctcc acggcaacgt agaacgcagt gatggggatg ccggttaggc gcgccagcgt
     2761 caccgggcct tttttggcga cgtagcgcgg accgcgggga ccatcaatgg tgaaagctac
     2821 ggcgcccccg gcttttacca tcgtctccat ttccagcaga ccgcgctggc ctccgcgcga
     2881 gctggacccg cgaactggta caaacccgaa gcggcgaatc acgcgcgcaa tatattcacc
     2941 gtcgcggctg aggctggtca gtactccgat atcgcgattg cggaacagcc aagtcgcggg
     3001 cagcacacag cggtgccaga agggataaat gcctggagca atgctgtcga ggccgcgaat
     3061 ggatccctct tcgtaggtga tggtcatacg cagcgtccgc ccgatgaggc ccaccaccgc
     3121 agacgcgatc cacgatacca gcgtgagctg catccgttgc agcagcgtga ggcggaactg
     3181 gtggatctcc ccggcggctg cgctggttgg attgggcacg cgagaattct aacctgtgta
     3241 ccgcagccag cggcgcaact cgggaagagt gggccgcttt ccatacatca gaatgccgac
     3301 ccggtagatg cgcgacgcaa gcgacagcag gccatagatg gtggccagca tcaaccccag
     3361 gcacaacgcc aactgccata ccggtggctc ctcgacgagg atgcgaacca gcatgagaat
     3421 gggcgcgaag aacggaatga tggacagcca gaaggcgagc tgcgagtttg gctgttgtat
     3481 cacgggattc atcaagaata ccgccaggat gatgggcacc atggccggcc actgcatctg
     3541 ctgcgcttcc tgatcggtgg tgaccatgga accgatagcg gcgtacatgg cgctgtacag
     3601 gaaatatccc aagacgaaaa acacgccgaa gaaaatgacg ccggcggttg ggatgtgaat
     3661 ctccgacacg aacgatttgg ctgccatcag gcccgagacg gcgaacgtca ccgcgaagat
     3721 gatccaaatc aaaatctggg ttagcccgac agcaccgacg ccgaggattt ttccggccaa
     3781 cagttctttg gcggtcacgg acgagagcaa cacctctatg atgcgcgaac ttttttcttc
     3841 gatgaccgaa cgcatcacgg caaccccgta aatcagtact acggcgtaga gcagcatcac
     3901 catggcgacg gcggtcgcga atatagcggc gccgctgacg ccctcttttc ctttttcaat
     3961 gtgcaccgca tcgagagtga tcggcgtcaa gagcgcctct acttccgggc cgctcatccc
     4021 cttctccgca agacgatgct ttgccaatgc ggccgtcacc gcgctgcgca attcgcgatt
     4081 tccccgaaaa tcggcagcat ccctggtgag gtaggtgacc ttgtgctgcg ccaccgcttc
     4141 atcggtgagc cacagaaagc catcgatctt gccgtcggtc acctgctgca gcaggtcgtt
     4201 gcgctcagct tcggtgggag tggtgctgat gccgacggca taactttccg tcgcttgccg
     4261 gtccgcttgg gaggtcgcgc ctggggttga ctcggtctcc aggagttgct gctccacggc
     4321 agtggcgatc tcgggattgc cggccacgat gacgatgcga cgcgcgccac ca
//
LOCUS       DATMGX010000516         3969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_149756, whole genome shotgun sequence.
ACCESSION   DATMGX010000516 DATMGX010000000
VERSION     DATMGX010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149756"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3786)
                     /locus_tag="VNR65_08360"
     CDS             complement(<1..3786)
                     /locus_tag="VNR65_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWJ18723.1"
                     /translation="MVRREPTEQKTSGSTGLLAAIVNSSFDAIIGKTLEGTVTSWNPA
                     AAKLFGYDPGEMIGASIRRLIPVDRQDEEDQMLSRIKTGEQVESYETVRLRKNGDPID
                     VFLTSSPIWGPDGKIIGASKIVRDVTSQKKAAETLRQSEERLRLFVEQAPAAIAMFDR
                     NMRYMACSDRWLPVHGRTEQSVIGRSHYEVFPEIPEGWKEVHRRGMAGEVVCSEEDAF
                     PRADGRTQWVRWELRPWLTGDQTVGGITIMAEDVTERVEAVRALRENELRTRLAQEAA
                     KVGTWELRLADNLSQWSQNIWGLYGLKPGHCTPSFESWTSSIHPDDRERNTKSLREAV
                     AIGREYEVQWRVNLPEGEPERWLLSRGRPVTDANGVPERYIGVVIDITEQKRAEEALQ
                     KAEERERQKREELEAVLAALPVAVIITKDAASVEMTGNQAAHELLRVPVNSSLSKSAP
                     PEQVPNNFEMFSKGRLLSTDELPIQKAASTKTIVHAEELEIRFAEGDSRIALCNALPL
                     FDAEGGVRGVVCVLTDITELKRTEAALRNSEEYLQFALEAANAGTWEVELSTGKFAAS
                     DGALYFLGIAPGTSVTLDITLSRVHPDDRPRLEEGLRHTLETGKPYRAEWRALLPDGS
                     IRWLETRGERRFASGKQLIGGLLQDITERKRAEQTLREREELLDSIIEHVPVPIILWR
                     EDRKVLLMNPALTKLTGYTASDIPTRDEWEALAYRDDAPQGTEDFRRTFESGRPIDLG
                     DRWVHTKSGEKRLWSLTRAPAGQDASGRRLVVGVAVDITERRKSEEEALATKSKLEAA
                     LAAMRDGVFILDAESRYIHFNEAFAKFHKYRSKEDSVQTFAELPAILNFFLPSGEWVP
                     RGEWPSQRALRGETEMNAEYTIKQREGEPYIGSYNFAPIRDSSGHITGAVVSVRDITE
                     QKRAANRLRESEARLSSIIDTAGDSIVVIDEKGIIQSANRATEGIFGYGLKDLVGRNI
                     GILMVPNLAAQHDRYIKGFSGRGAVREVGAKRKDGTMVPIDGTVAEWQDGEGRRFFTG
                     ILRDLTERKRNEEVLANARRLEAVGQLAGGVAHDFNNLLHVISGNLDIAQDFIDDETT
                     RTFLERARNAAEKGSALNGRLLSLARKRALKVERLDLNDRVQEIAKLLASTVGEHIAV
                     TTDLATGLWMTLADAGEIDSAILNLVANARDAMPGGGNVRISTSNVTLDAAAVKLHRK
                     AAPGDYVRLAIADSGVGMPEDVLDKATEAFFTTKEPGAGTGLGLTSVESFASQMGGFM
                     "
BASE COUNT          736 a         1229 c         1100 g          904 t
ORIGIN      
        1 catgaacccg cccatttgac tggcgaagct ttccacgctc gtaagcccaa gtccggtccc
       61 cgccccgggt tccttggtgg tgaagaatgc ctctgtcgct ttatcgagga cgtcttccgg
      121 cataccgacg ccactatcgg cgatcgcgag ccgaacataa tctcctggcg cagctttccg
      181 gtgcagtttt accgctgccg catcgagcgt aacattcgag gtcgaaatcc ggacgttacc
      241 gccgcccggc atcgcgtcgc gcgcattcgc gacgagattt aagatcgcgc tgtcgatttc
      301 gcccgcgtcc gcgagcgtca tccaaaggcc ggtggcgaga tcggttgtga ccgcaatgtg
      361 ctctccgaca gtactcgcaa gaagcttcgc aatttcttgg acgcgatcgt tgagatcaag
      421 acgctccacc ttgagtgcgc gtttgcgggc aagggacaag agccgtccat tcaaggcgct
      481 tcccttttcg gcggcgttcc gcgcgcgctc aaggaaggtc cgcgttgttt catcgtcgat
      541 gaaatcctga gcgatatcca gattgccaga gatgacgtgc agaagattgt tgaaatcgtg
      601 cgcgacgccg cctgccagct ggcccacggc ttcgaggcga cgcgcgtttg ccagcacttc
      661 ctcgttgcgt ttgcgctcgg tgaggtcccg caggatgccg gtgaagaaac gccggccttc
      721 gccgtcttgc cattccgcga ccgtcccgtc gattgggacc atggtgccgt ccttgcgttt
      781 cgccccgacc tctctgacgg cgcctctgcc cgagaagcct ttgatatagc gatcatgctg
      841 cgccgccaaa tttggcacca tcaggatgcc gatattgcgc ccgacgaggt ctttcagacc
      901 gtagccgaag atgccctccg tcgcgcggtt cgctgactgg attatcccct tctcgtctat
      961 gacaacaatg ctgtcgccgg ccgtgtcgat aatcgagctg agccgcgctt cgctctcacg
     1021 cagccggttc gccgctcgct tttgttcggt gatgtcgcga actgagacaa cagctccggt
     1081 gatgtggccg ctgctgtcgc gaatgggagc gaaattgtag cttccgatat agggttcacc
     1141 ctctcgctgc ttgatggtgt actccgcgtt catttctgtt tcaccgcgaa gcgctctttg
     1201 cgaaggccat tccccccgcg gcacccattc cccgctcggc aggaaaaagt tcaaaattgc
     1261 gggaagctcg gcgaacgtct ggacggaatc ttcctttgat ctgtatttgt ggaacttggc
     1321 aaaagcctca ttgaaatgaa tgtagcggct ctccgcatct aaaatgaaaa caccgtccct
     1381 catggccgcg agggcggcct ccaacttcga tttggttgcc agagcctcct cttcgctctt
     1441 tctgcgttcc gtgatgtcaa ccgccacgcc aacgacgagt cgccggcctg aagcgtcttg
     1501 ccccgccggc gctctcgtga gcgaccaaag gcgcttctcg ccggattttg tgtggaccca
     1561 gcggtcccca agatcgatgg gccgaccgct ttcaaaggtt cggcgaaaat cttccgtccc
     1621 ctggggagcg tcgtcgcggt aggccaatgc ctcccactca tcccgagttg gaatgtcgga
     1681 agcggtgtag ccggtaagct tggtcaaggc cggattcata agaagaacct tgcgatcctc
     1741 cctccacaaa attatcggga ccggcacgtg ctcgatgatg gagtccaaga gttcctcacg
     1801 ttcgcgcagc gtttgttcgg cgcgcttgcg ctcggtgatg tcttgaagca atccaccgat
     1861 gagctgcttt cctgaagcaa atcgccgctc tccgcgcgtt tccagccatc gaatcgaacc
     1921 gtcgggaagc aaggctcgcc actccgctct ataaggcttc cccgtttcaa gggtgtgccg
     1981 gagaccttcc tcaaggcgcg gccggtcatc cggatgtacc ctggagagag tgatatcaag
     2041 agtgaccgac gtgccgggag cgatgccgag gaaatagagc gctccatccg aggcggcgaa
     2101 ctttccggtt gaaagctcga cctcccaggt tccagcgttt gcggcctcaa gtgcaaattg
     2161 aagatactcc tcactgttgc gcagcgccgc ttcggtgcgc ttcaactcgg tgatgtcggt
     2221 gagcacgcag actacgccgc gaacgcctcc ttcggcatcg aagagaggca aagcgttaca
     2281 aagcgcgatt ctgctgtcac cctctgcgaa gcgaatttcc agctcttctg cgtgaactat
     2341 tgttttcgtg ctggccgcct tttgaatagg caactcgtca gttgataata agcggccctt
     2401 tgagaacatt tcgaagttat ttggcacttg ttccggcgga gctgacttcg ataggcttga
     2461 gttaacgggg acacgcaaaa gctcgtgagc ggcttgattg ccggtcattt ctacggaggc
     2521 ggcatccttt gtaatgatga cggcaaccgg taacgcggcc aggacggctt ccaactcttc
     2581 acgcttttgt ctttctcgct cctcggcctt ctgcagggct tcctcagccc gcttttgttc
     2641 ggtgatgtcg atgacaacgc cgatgtagcg ctccggaact ccgttcgcgt ccgtaaccgg
     2701 cctgccgcgc gaaagcagcc agcgctcggg ttcgccttcc ggcaggttca cgcgccattg
     2761 aacttcgtac tctcgcccga ttgccaccgc ctccctgagc gactttgtat tgcgctcgcg
     2821 atcatcagga tggattgatg acgtccagct ttcaaaagaa ggcgtgcagt gcccgggttt
     2881 aagcccgtac aacccccata tgttttggga ccactgactg aggttatccg ccagcctcaa
     2941 ctcccaggtc ccgaccttgg ccgcctcctg agccaaacgc gtccgcagtt cattctcgcg
     3001 caaggcccgc acggcttcga ccctttcggt cacgtcctcg gccatgatgg tgatgccgcc
     3061 aactgtctga tcgcccgtca gccaaggccg caattcccag cgtacccact gcgttcgccc
     3121 gtcagcgcgc gggaaggcgt cttcctccga gcacacgacc tcgccagcca tgccccgccg
     3181 atgcacctct ttccaaccct cggggatttc cgggaaaacc tcgtaatggg aacggcctat
     3241 gacgctttgc tccgttcgcc catgaactgg gagccagcgg tcgctgcagg ccatgtagcg
     3301 catgttgcga tcaaacatgg cgatggcggc gggtgcctgc tccacgaata ggcgcaagcg
     3361 ttcctcgctt tgacgcagcg tttccgcggc tttcttttgc gaggtgacgt cgcggacgat
     3421 ttttgaggcg ccgattattt ttccgtctgg cccccagatg ggtgaactcg ttagaaaaac
     3481 gtcgatagga tcgccgttct tacgaagacg gaccgtctca taggactcaa cctgttcacc
     3541 cgtcttgatc cttgacagca tttggtcttc ttcatcctgc cggtcgacag ggatcagccg
     3601 gcgaatggac gcgccgatca tttcgccggg atcgtagccg aaaagcttgg ccgcggccgg
     3661 gttccagctt gtgaccgtcc cttcaagcgt tttgcctatg atcgcgtcaa aggacgagtt
     3721 gacgatggcc gccaacagac ccgttgaccc ggaggttttt tgctccgttg gttccctgcg
     3781 gaccattgca tcccagcaat gagcgaggcc cctccctagc gaatgatgcc gcatgcgaag
     3841 ctgactccag gaccgtatgc aaggctttcg ccctgctgcg ccgatggcgg cgcctatcgc
     3901 ctagataacg ccggctgcga taataaacag ataaaaactt gtctacattc cgcttgtcag
     3961 gatgagcgc
//
LOCUS       DATMGX010000517         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_184520, whole genome shotgun sequence.
ACCESSION   DATMGX010000517 DATMGX010000000
VERSION     DATMGX010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184520"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VNR65_08365"
     CDS             complement(<1..303)
                     /locus_tag="VNR65_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18724.1"
                     /translation="MVPGYAIKIALAIGLAAAAALGAITLFIKVAATLSEEVPQQSAS
                     GKLQTEKRPTSPPEELSLNTAEDKPSSFAAPPATETFANNWKTGNEIKSPDIVALKP"
     gene            complement(650..1948)
                     /gene="purB"
                     /locus_tag="VNR65_08370"
     CDS             complement(650..1948)
                     /gene="purB"
                     /locus_tag="VNR65_08370"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWJ18725.1"
                     /translation="MIPRYSRPQMAAIWSDRSRYEIWLEIETLAAEAMEQLGQIPAGT
                     SKVLRERGAFDIERIDEIEREVKHDVIAFLTSVSEHVGPEARFMHQGMTSSDVLDTCL
                     AVQLTRAADILLADLDALLAALKRRAIEHKYTVTVGRSHAIHAEPTTFGLKLAFAYAE
                     FSRAKERLLRAKEDVGTCAISGAVGTFAHLDPSVEAYVASKLGLKPEPVSTQIIPRDR
                     HAMFFATLGVIASSVERLATEIRHLQRTEVAEAEEFFSAGQKGSSAMPHKRNPVLTEN
                     LTGLARLVRGFAVPAMENVALWHERDISHSSVERMIGPDATVTLDFALARLAGVIDKL
                     VVYPERMRANLERGCGLVHSQQVLLALTQNGVNREDAYRLVQRNAMRAFHGEGSFLEF
                     LKADAEVSAKLSPAEIDGLFDTRHHLKNVDVIFERVFASA"
BASE COUNT          407 a          577 c          637 g          423 t
ORIGIN      
        1 cggctttaga gctacaatat caggagactt aatttcgttg cctgttttcc agttattggc
       61 aaaagtttct gtagcagggg gggcggcaaa gctactaggt ttatcttcag ccgtattgag
      121 tgaaagttct tccgggggtg atgttggtcg cttctccgtc tgaagcttgc ccgaagcact
      181 ctgctgcggg acttcttcgg agagtgttgc tgctactttt atgaaaaggg ttattgcgcc
      241 aagggcggca gcagcagcca aaccgatcgc tagggctatc tttattgcgt aacccggcac
      301 catgagtctc cttcacgaag catccgacaa gataattgcc tatcggcatc gctaagaaag
      361 ccacttctta agattatcaa caacaagacg ccattcatgg tgactcggtt tacgctgcga
      421 gcctgcgggc gcttgtgcgc ttagttgccc ggatgctatg cgggaccagc gattcgtaag
      481 agccgcaaag aataagaccg cttccttttc gcggcctacg ggagatcacc aaggttgtga
      541 ttgggacact ttcggcgaac aaagaaagtg ccagatcacg gccctgaagc gtgagttcgc
      601 cattggccaa ctcggtgccg ctaagattgg tccaaccagg aactctgctt taggccgacg
      661 caaacacgcg ttcgaaaatg acgtcgacat tcttgagatg atgccgtgtg tcgaaaagcc
      721 cgtcgatttc cgcgggtgag agcttcgcgg agacttccgc gtcggccttc aggaattcga
      781 ggaaggagcc ttcgccgtgg aaggcgcgca tggcgttgcg ctggacaagg cggtaggcat
      841 cttcgcggtt cacgccgttc tgtgtaagcg caagcaaaac ctgctgagag tgcacgaggc
      901 cgcagccgcg ctccaaattc gcgcgcatcc gttccggata gacgaccagc ttgtcgatga
      961 cacccgcaag cctggcgagc gcaaaatcca gggtgacggt cgcgtctggc ccgatcatcc
     1021 gctcgacgga ggaatgggag atgtcgcgtt cgtgccagag cgccacattt tccatcgcgg
     1081 gcaccgcaaa gccgcgcacg aggcgtgcaa gaccggtcag attctcggtc aggaccgggt
     1141 tgcgcttgtg cggcatggcg gacgagccct tctgtcccgc cgagaagaac tcctcggcct
     1201 cggcgacctc tgtacgctgc aaatggcgga tctcggttgc tagccgctcc acggacgaag
     1261 ctatcacgcc gagcgtcgcg aagaacatcg cgtggcgatc gcgcgggatg atttgcgtcg
     1321 agaccggctc gggcttgagg ccaagcttgc ttgcgacgta agcctccacg ctcggatcga
     1381 gatgcgcaaa ggtgccgacc gcgccggaaa tagcgcaggt gccgacgtcc tcttttgcgc
     1441 gcagaagccg ctctttagct cgcgagaatt cggcataggc gaaggcaagc ttcaatccga
     1501 atgtcgtcgg ctcggcatgg atggcgtggc tgcggcccac cgtgaccgtg tatttgtgct
     1561 caatggcacg gcgcttcagc gcggcgagca gggcgtcgag atccgcgagc aggatgtccg
     1621 ccgcgcgcgt gagctggact gcgagacagg tgtcgagcac atcggaagaa gtcatgccct
     1681 gatgcatgaa gcgcgcttcc ggcccgacat gctccgacac actcgtgaga aacgcgatga
     1741 cgtcgtgctt cacctcgcgc tcgatctcgt cgatgcgctc gatgtcgaac gcaccgcgct
     1801 cgcgcagcac cttcgaggtg ccagccggga tttgccccag ctgctccatc gcctccgcgg
     1861 caagcgtttc gatttccagc cagatttcgt atctgctgcg gtcagaccag attgcggcca
     1921 tttgcggccg ggagtagcgg ggaatcattg gggcgctctt ttaggctgtg gcagccgcca
     1981 cgtatggcgc gatgctgagg ttgtgggatt gcaggagggc gagcagtgac gcggacggcg
     2041 tccg
//
LOCUS       DATMGX010000518         5364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_322408, whole genome shotgun sequence.
ACCESSION   DATMGX010000518 DATMGX010000000
VERSION     DATMGX010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322408"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VNR65_08375"
     CDS             complement(<1..617)
                     /locus_tag="VNR65_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18726.1"
                     /translation="MPQQPKPPSTKDAAHEIAPQESSRAEIDAFLAQVRSAPSPSTSG
                     NGRLIFAMDATMSRQPTWDMALQVQAQMFNAVKAVGGLDVKLVFFRGFDECKASRWVS
                     DATALARLMTAVECRGGFTQIRKVLKHALGEAANGKVSALVYVGDAMEENVDELANLA
                     GQLGLLSIPVFLFQEGYDGRAQGAFAEIARLTRGATCRFGPGSAA"
     gene            complement(766..2142)
                     /locus_tag="VNR65_08380"
     CDS             complement(766..2142)
                     /locus_tag="VNR65_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ18727.1"
                     /translation="MTDRLADAIKAGSASLEESASIFETFNAGSSDGARAVALAQADC
                     AGADRALADLESAAEPGQQASFIAAAGAQSAGFTTSAPAGSVRGVTANEVRFGTSLPL
                     SGPNKESGQQIKIGIETAFRMANDAGGIHGRMLRLVAADDGYEPTRTAETMKQLYDKD
                     QVFGFIGNYGTATAAVSAPFALDRQALFYAGFTGASVLRRDPPDRYVFNFRASYAEET
                     EAVVRYLVKVRRLKPEQIAVFAQQDAYGDAGFAGVAKAMRVLRGGDSGSILRLGYQRN
                     SVDVEPAVAQLKASKLPIKAVVMVATYRAAAKFIEKTADAYPGLTYTNTSAVGSISLR
                     DELMLLGPKYVSGVIVTQAVPAVDGYSSLILQYKAALAKYSPGQAPDYVSFEYYVATR
                     VLIEAMKRAGPQIDTEKLVDALESVRDLELGLGIPITFSVTDHQGSHKVWATQMTQDG
                     KYEPIDLQ"
     gene            complement(3526..3717)
                     /locus_tag="VNR65_08385"
     CDS             complement(3526..3717)
                     /locus_tag="VNR65_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18728.1"
                     /translation="MDLWTFLGFVAVLALLAAIEKQLIAIHKDLVETKVKIEYISALV
                     LNKDLPAAKADVKERVLAS"
     gene            complement(4022..>5364)
                     /locus_tag="VNR65_08390"
     CDS             complement(4022..>5364)
                     /locus_tag="VNR65_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18729.1"
                     /translation="ENGCEERSAATAFNTLTRSNIRAIGIGLEFRDMTAMIAEILKEG
                     KPAIDPDDIAGIRKSILKGIPALFASGKGAFKGVLEDWSFRPSSRTAVRTPFIIARPK
                     DLHRQQLSAIQYAPLKDNKLRKIPTLYLDIDLFRISRIDDTDKSFVADFYLSMSDENN
                     PSIDLIEFTNAFLDYKTNARQITIRPLHEGGESDSYPSHMKVYAVSGKFTLDPSYNSY
                     PFDVQRFTIDLRPKRGDSPFIVQPLQQELREKPFDTGGWDNKGSYVSYDQDSISVFDT
                     KKLEPSVVPFYNSSFVWVLKRSATDFYIRVVIPLFFIAIVAYLAIFISSAHFESIVTI
                     QVTAFLSAVALYITTPKVDSDTATILDRIFVFNYMIFSLMIVISILRVNKFIASTLHL
                     KRALAVVHVVFIPIFLGVMVFYVYGVGGSESHSELEFGPALRDGFSRFLVWLGA"
BASE COUNT         1193 a         1548 c         1458 g         1165 t
ORIGIN      
        1 tgcgccgccg atcccggtcc gaaacggcat gttgctccgc gcgtcagccg cgctatttca
       61 gcgaaggccc cctgcgcccg gccgtcgtag ccttcctgaa aaaggaagac gggaatggac
      121 agaagcccga gttgccccgc cagattggca agctcgtcca cattctcttc catggcgtcg
      181 ccgacataga cgagcgcgct cacctttccg ttagccgcct cgcccagtgc atgcttcagc
      241 actttgcgaa tctgagtgaa gccgccccgg cattcgaccg cagtcatgag ccgggcaagc
      301 gcggtcgcat cgctcaccca gcggcttgcc ttgcattcgt cgaagccccg gaagaagacg
      361 agctttacgt cgaggccgcc gaccgctttc accgcgttga acatctgggc ctgaacttgc
      421 aacgccatgt cccaggttgg ctggcggctc atggtggcgt ccatggcgaa gatcagccgg
      481 ccattccccg acgtggaagg cgacggagcg gagcgcacct gcgccaggaa ggcgtcgatc
      541 tcggcgcggc tcgattcctg cggcgcaatc tcgtgcgccg cgtcttttgt ggacggtggc
      601 ttgggctgtt gcggcatggc gaatccggga gctattcgcc tttatatggt tggcttcagc
      661 caaatgcgag ccccggctca ccgaccgttg ctgacttttt ggggacgggt taccaacccg
      721 ttccgcctta ccgcgccaaa gcggctgcat tattcgcctg cgtcgctact gcagatcaat
      781 cggctcgtat tttccatcct gggtcatttg cgtggcccaa accttgtgcg atccctgatg
      841 gtcggtcacg ctaaaggtga ttggtattcc aagccccagc tcaaggtcgc gcacgctttc
      901 caaggcgtcc accagctttt ccgtgtcgat ttgcggcccg gcccgcttca tcgcctcgat
      961 aagcacccgt gtggctacat aatattcgaa ggaaacatag tcaggagcct gaccgggcga
     1021 atacttcgcc agcgccgcct tgtactgaag tatcaatgag gagtagccat ccacagccgg
     1081 cactgcctgc gtgacgatga cgcctgacac gtattttggc ccgagcagca tgagttcatc
     1141 gcgcaaagag atgctcccga cagccgaagt gttggtatac gtcaagccag gataggcgtc
     1201 tgccgtcttc tcgatgaatt tcgctgccgc ccgataggtc gccaccatca ccaccgcctt
     1261 gatgggcaac ttgctggcct tcaactgcgc gactgctggt tcaacatcta cagaattgcg
     1321 ctgatatcct aagcgcagaa tggaaccgct atcgcctccg cggagcaccc gcatcgcctt
     1381 cgcgacacct gcgaagccgg catcgccgta agcatcttgc tgagcgaaca ccgcgatttg
     1441 ctccggtttt agccgccgca ccttgaccag atagcgcacg acggcctctg tctcctccgc
     1501 gtagcttgcg cgaaagttaa agacataacg gtcgggcgga tcgcggcgca gtacgcttgc
     1561 gcctgtgaac ccggcgtaaa acagcgcctg ccggtcgagc gcgaagggcg cagacaccgc
     1621 ggccgtggcg gttccgtagt tcccgataaa gccaaacacc tgatctttat cgtagagctg
     1681 cttcatagtc tcagcggtcc gcgtgggctc atagccgtcg tcggcggcta cgaggcgcag
     1741 catgcgtcca tggataccgc ctgcatcatt ggccattcga aaggcggtct cgatgccgat
     1801 ctttatctgc tggccggact ccttgttagg ccccgagagc ggtagcgatg tcccaaaccg
     1861 gacttcattt gccgtcacgc cacggactga tccggcggga gccgaagtgg tgaagcccgc
     1921 agattgagcc cccgccgcgg caataaatga agcttgttgc ccaggctcgg ctgcgctttc
     1981 aagatcggcc agtgcgcggt cagcccctgc gcaatccgcc tgcgccaacg caaccgcacg
     2041 cgcgccgtcc gatgagcctg cattgaaggt ctcgaagatg gatgcgcttt cttccaggga
     2101 tgcagatccc gccttgatcg cgtctgctag ccggtcggtc actttcttaa ttcgctctcg
     2161 tgaaacattc gggcaagcgg atgcggtccc aagcacgtga ccgacacgcc cggcgagccc
     2221 ccgaaggaca tttgctttgt catcacgagg cgccgaaata gaagagaccg gcaaaagcag
     2281 caaaagcgtg catgccgaaa tagccccaaa tcgaaaattc accccaccct ccttaataac
     2341 gagaacttct cgctgcggta gcatcgaaga acaagctcaa ccgtgtcgcc tggcaacctg
     2401 ggaagcagaa tcactcgcgc tcactcgccc ctttactcct cgcaatccgt cgtagacctg
     2461 cgtctctggg acgcagcact tatacttttc tgaataaact gtgtatgagt gcaggcattc
     2521 ccggttggcg ctcctgtttg ggagcgcccc gagaaattag aactgcaaaa aagccttgcg
     2581 caattgtacc gtttacagac ggcatgctag cttgcagcca aggtcgatcg ggagttgagg
     2641 tgatccgcta atgctgcgca ctgtcacaac cgtgataatg atgaccttca tcctgcccca
     2701 ggcacacagt gaggaggcga ttgcccccag tccgtattgg agcgccatta cctcggctcc
     2761 aggaagcgca atcgccaaaa gtcccgaagt gattcgtgtg caagaacctt tttgcgagta
     2821 tggcgtgggg ccatgcggag gaacctgcag cgaggagggc ggaaagcact gggtttgtgc
     2881 gaccactgaa atgccatgct accagctcgg ccgttgcaag tgcgaagctg cggctatttg
     2941 caaaccgccg ccgccgaaaa agaaaaagat caacctgggt gacaaataga cgtgggatgc
     3001 cgtcttgccc gggatttggc agcgccgcga taatgagctt gctgcccgca atgattgcct
     3061 acggactcaa atcaaatggt gcaagcgctt gaaaatctcg tctgtgcgcg agactacatc
     3121 gcgcatgact ccggtgcttg gcgcggcctt gctgttcgca gattcgcggc cgtcctgagt
     3181 tgaagccagg gcgcaaacag gctctaaaag gagcaggtgg atttggtggt ggagcctaat
     3241 cggccaagcc gccagtgtag atcgatcagc aactcacgca gaagcgagcc ctcgctgctc
     3301 ttgagcaggc aagcgaggcc cggcgcagca ggatccggta ggcaacatcg atatctcgcc
     3361 aatccaatga aacgtgattt tcgtgttgct cccctgatcc tggcggtcac ggccatgatc
     3421 cactgttgag tgcgcgcgcc aacaaccacg gcacctaccg agtttcgtta gatcgcgctc
     3481 gattgatcgt caaagcgccg ttgtcctgac cgtctgcctc gtgttctatg aggccagcac
     3541 gcgttccttg acatcggctt ttgccgctgg gagatcctta ttcaaaacta gcgcgcttat
     3601 gtattcgatt ttcactttgg tttcgacgag gtctttatga atcgcaataa gctgcttctc
     3661 gattgctgcg agtaaagcta ggacagcaac aaaaccaaga aatgtccaaa ggtccatctt
     3721 tttctcctgt gattactgcc taatcgtctg gcgagtggag tgcacttcat gcgattatga
     3781 aacccagcgg cattgacaca gcgctcaagt ttcacacccc acgagaaagc ggtccatctc
     3841 cttagcttgc gctccccttt gcggcgcggc gtcatgttag agctttatcg acgccgtggc
     3901 cagttctcgg tcgatggaag aacgcgaatt ttcgacgcta aatgacgcag aaaaagggta
     3961 gcggcatcac agccttgatt ggcgggcctg taaatgcgtg caccgcgcta cgctgtcgat
     4021 tttaggctcc caaccaaacc agaaagcgcg aaaagccgtc ccgcaaagcc ggcccaaatt
     4081 ccagctccga atggctttcc gatcctccta ccccgtagac atagaagacc atgaccccaa
     4141 ggaagatagg aataaagaca acatgcacaa cagcaagcgc ccgtttcaaa tgcagcgtgc
     4201 ttgcgataaa cttgtttacg cgcaagatcg aaatcacaat catgaggcta aaaatcatat
     4261 agttgaagac gaatatcctg tccaatatcg tagctgtgtc tgagtctact tttggcgtcg
     4321 taatgtaaag cgcaaccgcg gaaagaaatg cggtcacctg gatcgtaact attgattcaa
     4381 aatgagcgct tgaaatgaaa atcgccagat aggcaacaat tgctatgaaa aataacggta
     4441 tcacgaccct gatatagaag tcggtcgcgc tgcgcttcaa gacccagacg aagcttgagt
     4501 tgtagaacgg aacgacactc ggctcgagtt tcttggtgtc gaaaaccgag atggaatcct
     4561 gatcatagct tacatacgag cccttgttgt cccagcctcc ggtatcaaac ggtttttctc
     4621 gcagctcctg ctgaaggggc tgaacgatga atggggagtc tccgcgtttc gggcgcaagt
     4681 cgatcgtgaa acgctgcacg tcgaaaggat agctgttgta actcggatca agcgtaaact
     4741 tcccagatac ggcgtagact ttcatgtgac ttggatagga atcgctttcc ccgccctcgt
     4801 gcaggggccg gatcgtgatt tgcctcgcgt tggtcttgta atcgaggaaa gcattcgtga
     4861 actcaatcaa gtcgatggag ggattattct cgtcgctcat cgataaataa aagtctgcaa
     4921 caaagctctt gtccgtgtcg tcgattctgg aaatacggaa caaatcaata tccaggtaga
     4981 gggttgggat ctttctgagc ttgttgtctt tcaagggagc gtattgaatc gctgaaagct
     5041 gctgtctgtg caagtctttc gggcgcgcaa tgataaaagg tgtcctgacg gctgtccggg
     5101 aggaaggacg gaacgaccag tcttcgagaa cccccttgaa agcacccttg ccggacgcaa
     5161 acaaggcagg gatccctttc aaaatcgatt tgcgaatccc tgcaatatca tcgggatcaa
     5221 tcgccggttt cccctccttc aagatttcgg cgatcatcgc ggtcatgtcc ctgaactcca
     5281 accctatccc aatggccctg atgttggagc gagtcagagt attgaacgcg gttgccgccg
     5341 agcgttcctc gcatccattt tcgg
//
LOCUS       DATMGX010000519         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_15058, whole genome shotgun sequence.
ACCESSION   DATMGX010000519 DATMGX010000000
VERSION     DATMGX010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15058"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..743)
                     /locus_tag="VNR65_08395"
     CDS             complement(381..743)
                     /locus_tag="VNR65_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWJ18730.1"
                     /translation="MNIAPIKTQRDYRRVLKEIESLMTAKRNTPEGDRLDVLVTLVEA
                     WERKHYPLDLPDPVDAIKYHMDQNGLQPRDLIPFIGSRNRVHEVLNRRRPLTLKMIWR
                     LHEGLGIPAESLIKKRAA"
     gene            complement(755..1081)
                     /locus_tag="VNR65_08400"
     CDS             complement(755..1081)
                     /locus_tag="VNR65_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HWJ18731.1"
                     /translation="MGLCYVGSVRIIALSTLKAFLNRRPAYAEAQEPVMAWHRQVRGA
                     DWATPADVKRAIRSASILKDGRVVFNIAGNKYRIVVWINYPYRVVYIRFIGTHRQYDA
                     INAQTV"
     gene            1592..1894
                     /locus_tag="VNR65_08405"
     CDS             1592..1894
                     /locus_tag="VNR65_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18732.1"
                     /translation="MKMLFFILAFTTLAIANPAMAQYSAPDSTPGYNWREQRGSQDWR
                     NNTWREQQVDQDWRNNQWRQQRANEDWQQREKYMKKRTPNNATDTGYAEDKSNEKE"
BASE COUNT          509 a          584 c          637 g          514 t
ORIGIN      
        1 atgggccttg tcacgcagcc gcttgggctc ggcgatggcg agaacgctct tgtcgatttg
       61 cgcagggacg aggccgggtg aggcagggac aattatcgaa gtgtcaactg atggccggtc
      121 ttccagttca cgtcgaccct gcgctcgctc ccgcgtgacc atatcctgac ggcggccata
      181 gtagcgggac ggtcgtggaa taggtgtcgt atcgtccgga tggaaaccga ggcgggcgtc
      241 ggcggatatg gcggtgatcg gggctgcgcc gcagcgcctt tacttgcttt gttgagaagc
      301 ccatcaccgt gtcctcacgc tcaataccgg ttccggattg gagagggtgc accacgccgt
      361 tgtgatttca tttctacgcg tcaggccgcc ctttttttga tgagcgactc tgcgggaatg
      421 ccgagcccct catgcaggcg ccagatcatc ttgagcgtaa gtggtcgtct gcgattgagt
      481 acttcgtgca cgcggtttcg gctgccgatg aagggaatga gatcacgcgg ctgaagcccg
      541 ttttggtcca tgtgatattt gatcgcgtcc accgggtcgg gcagatcaag cggataatgc
      601 ttccgctccc aggcttccac tagcgtgacg agcacatcaa gccggtcacc ctcgggagtg
      661 ttgcgtttcg ctgtcatcaa gctttcgatt tccttgagga ctcggcggta atcacgctgg
      721 gtcttgattg gtgcgatgtt catggcttta gccttcaaac ggtctgtgca ttgattgcgt
      781 cgtactggcg gtgtgtccca atgaaccgga tgtagaccac ccgataggga taattgatcc
      841 acaccacgat gcgatattta ttgccagcga tgttaaacac cactcggccg tccttgagga
      901 tgcttgcgct gcggatcgca cgttttacat ccgctggcgt tgcccaatcg gcgcccctga
      961 cctgccggtg ccacgccatg accggctctt gtgcttcggc ataggcgggc cttcggttga
     1021 ggaaagcttt gagcgtgctg agcgcaataa tcctcacact cccaacgtag catagtccca
     1081 tattgggacc aagagtttat ttgctagttt ggtctcaaac ccaactggta gagcaggctc
     1141 tcgcgccgca agtttgccat tcgcgcgctt gatgcagtgc gtcagcaagc cgccacataa
     1201 gcagggcggt caacaagctg acctaagagc caggccagcg atccctaaaa ggtcacctag
     1261 caggatggac cggtaaaatc gtcgttctcg gtcgggtcaa tgctgccagt gggccctatg
     1321 cagcgccggt gaaggtcgaa aagccccgcg gtctattggc gtcgatgtca gcgctattca
     1381 tcgcgccttg ttgatccgca tcaatgcgat taacatatcc ggcgaagtcc cttggctacg
     1441 agttcgatcc tcgtattctt tctcctttgg cccactgacg cctcagcctc tttttgccat
     1501 tggtctcggc gcgttaggtc tgctcggatg gcacgggata cgaaaggcct cgggcagggt
     1561 tatttctgta ggtgacgatc aggagataac tatgaaaatg ttgtttttca tattggcctt
     1621 caccaccttg gcaattgcga atcccgcaat ggctcaatat tcggcgcctg attctactcc
     1681 cggttacaat tggcgcgaac agcgcgggag tcaggactgg cgtaacaaca cctggcgaga
     1741 gcagcaagtc gatcaagatt ggcgtaacaa tcaatggcga caacagcgcg cgaacgagga
     1801 ttggcaacag cgcgaaaaat acatgaaaaa gagaacgcca aacaatgcaa cggatacggg
     1861 atatgccgag gataaaagta acgaaaaaga ataatgcaaa gaacgaagaa tgcgccaacg
     1921 tcgttgtggg agcgctagcc tgaccgggct tttttagacc aagcgatgtg agaaattgag
     1981 cttggaaaag agctctgatc acgccgaaga agaggttcta cgtggagcag atcgtaacgc
     2041 tgcttcgcca gattgaagtg ttgatggagc aggtaaatct gcccggcagc cgccgcataa
     2101 gcccaaagat gacgccgcaa attatgatcg aaagacttct aataatatag gaagatattc
     2161 tacattaatg atcattttgg caagacgaac ccaaactcga taaactcaaa atcacatctc
     2221 cctatggtcg cgcggggcag ggct
//
LOCUS       DATMGX010000520         3550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_196324, whole genome shotgun sequence.
ACCESSION   DATMGX010000520 DATMGX010000000
VERSION     DATMGX010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196324"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VNR65_08410"
     CDS             complement(<1..386)
                     /locus_tag="VNR65_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWJ18733.1"
                     /translation="MLTFLLSAAASAQKINGVPGSPSATTTIPGNQLPALPEKFGGKI
                     DREATKSTPYWPARVVPPKGAPNVLLIITDDSGYGVPSTFGGVIPTPALDRIAQDGLR
                     FTNFHSTALCSPTRAALITGRNHHSVG"
     gene            complement(865..2850)
                     /locus_tag="VNR65_08415"
     CDS             complement(865..2850)
                     /locus_tag="VNR65_08415"
                     /inference="COORDINATES: protein motif:HMM:NF019874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HWJ18734.1"
                     /translation="MHTMRFSIGLRIYSIIGLSFCGLIGLAVMQAGNLADSLKQQREN
                     ELSHLTQLALSIAREEYETAVRDHSSEELARKTAAARISKLRYGNGDYFWINDLGPRM
                     IMHPLKPELNGQDLTENKDPNGKRLFVEFANVVKKQGSGFVEYQWPKPGKDAPQPKLS
                     YVTGFEPWSWVIGTGVYIDDLQAQLWDSARKAVIVALVIIALLGTVTLIIARKMSKAL
                     VAMTSAVTKLGQGDFGIELPGLDRGDELGDMARSIEQFKVKAAEKARHEALMEEERRR
                     VTEQNKAMALKEMAETVERETNKAVGEVAAGTDLMARNSALMNDTALLLGKNSSSVAA
                     AAEEALANAQTVAKASSQLSASIAGIATQVNSSRQLTLEAVTASTKAQATIGKLSEAA
                     AKVGAVTSLISEIAGQTNLLALNATIEAARAGEAGRGFAVVAAEVKSLAEQTAKATSE
                     IAQQITEIQDATQASVASISVIGEVIRSVEAVSSTIAEAIEEQNTVTVEISRTVEETS
                     LAAREVASQIVSVSNEATETGRSATEIRDGSVEIAAKVDALRATLVRVIRTSTSDVDR
                     RLSSRLDIRRSATLKMQGRSRTVTIRDLSLGGAMIDETLPNAPINTPVSLAIDGIEAE
                     LAGVVARKEENATLVTFQLSEEATKVLRGLISIQQAA"
BASE COUNT          684 a         1035 c         1042 g          789 t
ORIGIN      
        1 cctaccgaat gatggttgcg gccggtgatc agggcggcgc gggtcggcga acacagcgcc
       61 gtcgaatgga agttggtgaa tcgcagcccg tcctgggcaa tccggtcgag cgccggcgtc
      121 ggaattacgc cgccgaaggt cgagggcacg ccgtagcccg aatcgtcggt gatgatcaga
      181 agcacgttcg gcgcgccctt gggcggcacg acacgcgccg gccaataggg cgtcgacttt
      241 gtcgcctcgc ggtcgatctt gccgccgaat ttctcgggta acgccggaag ctggttgcca
      301 ggaattgtcg tcgtcgcgct gggtgagccc ggcacgccgt tgattttttg cgcgcttgct
      361 gccgcggaca acagaaaggt cagcatcaat gtcgctacgc ccaatcgccc aatgctcatg
      421 acaaatcttt ccagtttgac aagtactcat cgctgagcac accaaagcat ttcacaaact
      481 ggatgacccc caacgtgacg ccttgattta gatcaagagt ttcgacggca tcgtgaaacc
      541 gcccgtgtcc gctaacgcca aacaatccgg cacttctttc taacgttgcc gtaaagtagt
      601 tcaaaccgtt ccggtagcgg caccatcggg aaaaactgaa gacaagcgcc gccagccgag
      661 taatcgatga gagtacaagc catcggtggt gatttcggag ctacgatgac ttttgctccg
      721 ctggtcatct gtccccctgg ccgaactctt ttatacaaac gcggctgtga cattgatcct
      781 tactctgatt tcctatgaat gcgatggagt tcgctgagca agcgtcgttg atgcttttga
      841 cgtaaatggc acgcgacgca agcatcacgc ggcttgctgg attgaaatta ggcctcggag
      901 gaccttggtt gcttcctcag atagttggaa tgtcaccaat gttgcgttct cctctttacg
      961 agcgacaacg ccggccaact ccgcctcaat gccgtctatt gcaagtgaga ctggggtatt
     1021 gatcggcgcg ttcgggaggg tttcatcgat cattgcgccc cccagcgaga gatcgcgaat
     1081 ggtcaccgtt cgtgatcggc cctgcatctt cagggtggcc gaacgccgga tatccaggcg
     1141 ggaggaaagc cggcggtcga cgtcggacgt ggatgtgcgg atcacccgca ccagggtagc
     1201 gcgcagcgcg tcgaccttgg cggcgatttc gaccgagccg tcgcggatct cggtggcgct
     1261 ccggccggtt tcggtggcct cgttcgaaac cgaaacgatc tgcgaggcga cttcacgggc
     1321 cgcgagcgag gtctcttcga cggtgcggga aatttcgact gtgacggtgt tctgttcctc
     1381 gatcgcctcg gcgatggtcg aggacaccgc ttcgacgctg cggataacct cgccgatgac
     1441 actgatcgag gccaccgagg cctgcgtggc gtcctggatt tcggtaattt gctgggcgat
     1501 ttcgctggtg gctttcgcgg tctgctcggc cagcgatttg acctccgcgg cgaccacggc
     1561 aaacccgcgt ccggcttcac cggcgcgcgc ggcttcgatc gtggcgttga gggcgagcag
     1621 gttggtctgt ccggcgatct cgctaatcag gctcgtcaca gcaccgacct tggcggcggc
     1681 ctcggacagc ttgccaatgg tggcctgggc cttggtcgag gccgtcacag cctcgagcgt
     1741 caactgccgc gacgaattca cctgggtagc aattccggcg atggaagcgc tcaattgcga
     1801 agaggccttc gcgacggtct gggcgttggc gagagcttcc tccgcggccg ccgcgacgct
     1861 gctgctgttc ttgccgagca gcaaggccgt atcgttcatc agggccgaat tccttgccat
     1921 caggtcggtt ccagcggcga cttcgccgac cgccttgttg gtttctcgct cgacggtttc
     1981 agccatttcc ttcagagcca tggctttgtt ctgttcggtc acccgcctcc gctcttcttc
     2041 catgagcgcc tcgtgacggg ccttctcggc ggccttgacc ttgaactgtt cgatcgagcg
     2101 agccatgtcg cccagttcgt cgccgcgatc cagcccgggc aattcgatac caaagtcgcc
     2161 ttggccgagt ttggtgacgg ccgacgtcat ggcaaccagc gccttggaca tcttgcgcgc
     2221 gataatcagg gtaaccgtgc ccagcagggc gatgatgacc aacgcgacaa tgacggcctt
     2281 tcttgcgctg tcccagagtt gggcctgcag atcgtcgatg tagacgccgg taccgatgac
     2341 ccagctccac ggctcaaagc cggttacgta ggacagcttc ggctgcggcg catccttgcc
     2401 gggctttggc cattgatatt cgacgaagcc cgatccctgc ttttttacga cattggcgaa
     2461 ctcgacgaag agcctctttc cgtttggatc cttattctcg gtgaggtcct ggccattcaa
     2521 ctccggcttc agcggatgca tgatcattcg cgggccgagg tcgttgatcc agaaataatc
     2581 gccattgcca tagcggagtt tcgagatgcg ggcggctgct gttttgcgcg ccaattcctc
     2641 tgacgaatga tcgcgcaccg ctgtttcata ttcctcgcgg gcgatgctca aggcgagctg
     2701 agtcaaatgg ctcaactcat tttcgcgctg ctgttttagc gagtccgcga ggtttccggc
     2761 ctgcatgacc gccaagccga tcagtccgca aaagctgaga ccgataatgc tgtagatgcg
     2821 aagcccaatg gaaaaacgca tggtgtgcat caaccgttgc tggagcaata actgtccgat
     2881 tcggacggtg ccactctctc agccggaagc tgcagcaaag ttaaagtgtg tcatatttgg
     2941 taacccgtat aactacggga agcctactct aagaatgcca cggccattca actaatgaca
     3001 ggccccgcgc ctgctccaaa atctcgttgc aaatcgccga gttcggtttc gggtcaatcg
     3061 cgccgctttg acccaatgcc gattacttcc ggtgtccccc gatcaacgga cattctcagg
     3121 gtcagtcggc atgtctcaaa cgtgccaatc tgcgacattg cacgcgaacg tcgaggatta
     3181 gtcgaaggcc acgccgatcc gctcctcttg gcgccagaca acgtgacagg gaatgtgcgc
     3241 accgtcagcc ttaaagacaa ggttgaaacg ctcgggaact tcgtgggggt cggcgactcg
     3301 agcgccgccc ctgaaatgga catgtcgcaa actaagcaat taattgcgcc gccagcgaac
     3361 tgaatcgcgc cagacatcaa tacaggtttg cgagggatcg cgcgtctttc catgacacct
     3421 tgatgcgttg aaactcttag gaggttgggc ctatgtgttg aatttgcttt agcggcgcaa
     3481 tgtgagtgtt gtcggacact tttcgggctg ccgaccaggg ccttcagcgt ggattaatgg
     3541 caatcggatt
//
LOCUS       DATMGX010000521         3062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_174476, whole genome shotgun sequence.
ACCESSION   DATMGX010000521 DATMGX010000000
VERSION     DATMGX010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174476"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..1637
                     /gene="mltG"
                     /locus_tag="VNR65_08420"
     CDS             372..1637
                     /gene="mltG"
                     /locus_tag="VNR65_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWJ18735.1"
                     /translation="MAGDVYNSNGNGRTPNTAYRGQRDYSVMPRSPSEALEPERAPEP
                     PQGSRQGRQRPIVVFFSRLLSFFLVLLPAVAGLFFLIRQQFDRPGPINYPTVFVVAKG
                     EGVSTIARRLEQDGIINSDSLLPGRWIFLIASRRFGVHDKIKAGEFHIKANASVRDIL
                     DTLVEGKSILYSISIPEGLTSYQIVERMRASADLVGDVAEIPAEGSLLPDTYRFARGT
                     SREELIRRMQGEQKKFMEGLWPTRSRELSLTKPEEVINLAAIVEKEAARADERPRVAA
                     VYLNRLKKRMHLDADPTIIYGASDGKGSLGHPIKRSELENRENPYNTYRNYGLPPTPI
                     ANPGRAAIEAVLKPDKTAALYFVADGTGGHVFAETLNEHQRNVARWRHIEKEAREEER
                     MQDNSAGASIAIDKLQTVEIPLPQRNPRR"
     gene            1860..2564
                     /gene="gmk"
                     /locus_tag="VNR65_08425"
     CDS             1860..2564
                     /gene="gmk"
                     /locus_tag="VNR65_08425"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWJ18736.1"
                     /translation="MGHADQSLDRRGLCLILSSPSGAGKTTLARLLLSSDAALRNSIS
                     VTTREPRPREKEGVDYHFVSRKQFEDLKSSGNLLESAEVFGNLYGTPAEPVRGALAEG
                     RDILFDVDWQGAASIAALLPKDTVRVFILPPSAEELARRIHARASDSAHQIETRLKAA
                     AVEIAHWTEYDYVLVNRNVDECLAALKAILAAERLKRHRQVGLLEFVPALTSASRLGA
                     VSAPEDTERDAQNRKR"
     gene            complement(2488..>3062)
                     /locus_tag="VNR65_08430"
     CDS             complement(2488..>3062)
                     /locus_tag="VNR65_08430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HWJ18737.1"
                     /translation="MDALQADYEAIAAETGARRVVANLPYNIATPLIVGWLSAENWPP
                     WFDKLVVMVQREVADRFVAESGTADYGRLAVLTQYRARARLLLTLPPAVFTPPPKVSS
                     ALVEITPTAPPQDAVPVKFLEELTAAAFGQRRKMLRSSLSALGGDTAALLKDADIDPS
                     LRAERLTVSDFVRLARCLQEQKRHPIEKLK"
BASE COUNT          648 a          882 c          923 g          609 t
ORIGIN      
        1 ggcgaaagcg cgatcgctcc catgacctgc cagcgggcgc tcgtcagcgc gatatcggcc
       61 acaagcgtgt cgccggcttc cagcaatcga ccattcaggc gaaaaatctc cagaatgagt
      121 tcgcttatcg cgtcgaaaga cggggagtgg ggatgacggg gctcagccat ttgacacaat
      181 catgtcagtt tgacagtatg atgtcaaata tagcaaaaag gggatctttc tccctccgtc
      241 gccggagacc cgtacggccg gtgtacaagc ctttgtttcc ttgtataatt gggtgcgaga
      301 agagattcac accgtttcgc cacattggat tggtgaatgg ctatattcag gggcaagagt
      361 aagctgatcc gatggcgggc gacgtctaca actcgaatgg gaacggccgt actccgaaca
      421 cggcctatcg agggcagcgg gactactccg tgatgccgcg cagcccatcc gaggccctag
      481 agccggaacg cgctcctgag ccgccacagg gaagcaggca aggacgccag cggcccatcg
      541 tcgtcttctt cagtcggctg ctgagctttt ttctcgtcct tcttccggct gtggcggggt
      601 tgttcttcct tatccggcag cagttcgatc ggcctgggcc gatcaattac ccgaccgtat
      661 ttgtggtggc caagggtgag ggggtcagca ccatcgcccg ccgtctcgag caggatggga
      721 tcatcaacag cgacagtctg ctgccgggtc gttggatttt cttgatcgca tcccgccgtt
      781 tcggcgtcca cgataagatc aaggctggcg aatttcacat caaagcgaat gcaagcgtcc
      841 gcgatattct ggatacgctc gtcgaaggga agtcgatcct ttacagcatc tccattcccg
      901 aggggttaac gagctatcag atcgtagagc gcatgagggc cagcgcagat ctggttggcg
      961 atgttgccga aattcctgct gaaggcagcc ttctccccga cacctaccgc tttgcgcgtg
     1021 gtacgtcccg cgaagaactc attcgccgca tgcagggaga gcagaaaaag ttcatggaag
     1081 ggctgtggcc gacccggtcg cgcgaacttt ctctcacgaa gcccgaggaa gtcatcaatc
     1141 tggctgccat cgtagaaaag gaggcagccc gcgctgacga gcgcccgcgc gtggccgccg
     1201 tctatcttaa ccggctgaaa aagcgtatgc acctcgacgc cgatccgacc atcatttatg
     1261 gggcgagcga cggaaaaggg agcctgggcc accccataaa gcgcagcgag cttgaaaaca
     1321 gggaaaaccc ctacaatacc taccgcaact atgggcttcc gccgacgccg atcgcaaatc
     1381 ccggccgcgc tgccattgaa gccgtgttga agccggataa gaccgccgct ctctactttg
     1441 tggcggatgg tacggggggc catgttttcg cggaaacgct gaacgagcat cagcgcaacg
     1501 ttgcgcggtg gcggcatatc gagaaagagg cgcgagagga agagcgcatg caagacaatt
     1561 ccgctggggc atcgatagca attgacaagc ttcagacggt tgaaatcccg ttgccgcaac
     1621 gcaatccgcg ccgctagagc aacgtgcgcc catttggacg ccgaaactgc tcagcatttt
     1681 ccgcctttaa tcgattccga ttgaaggcgg gttgatctaa ggctgctcgc tgtctcgccg
     1741 caaaattgcg ctggaccttc cgtggaaatg ctgaaacaac cgacgtgccg gttgtcttcg
     1801 ccaatgaaga acccgggcat tgagcttaat gcaattgcct ttctcgtgga aaatccatcg
     1861 tgggtcacgc tgatcaatcg ctcgacaggc gcggtctctg cctgattttg tcatcgccat
     1921 ccggagcggg caaaaccact ttggctcgcc ttctgctgtc gagcgacgcg gcgcttcgca
     1981 attcgatttc ggtgacaaca cgggaaccgc ggccccggga gaaggagggc gtcgactatc
     2041 atttcgtctc acgaaaacag ttcgaggatc tgaaatcgag cggcaacctt cttgagtcgg
     2101 ccgaagtctt cgggaacctg tatggaacgc cggccgaacc cgttaggggt gcgctcgccg
     2161 aaggccgcga catcctcttt gatgtggatt ggcagggcgc agcgtccatc gccgctcttc
     2221 tgccaaagga caccgtgcgg gtctttattc ttcccccttc cgccgaggag cttgcaaggc
     2281 ggatccatgc gcgggcgtcc gactcggcgc atcagatcga gacccggctt aaggccgccg
     2341 cggtcgagat tgcccactgg accgagtacg attatgtgct cgtgaatagg aatgtcgacg
     2401 aatgcctggc tgcgcttaag gctattctgg cggccgaacg cctcaagcgt cacaggcagg
     2461 tgggcttgct ggaattcgtt cccgcgctta cttcagcttc tcgattgggt gccgtttctg
     2521 ctcctgaaga caccgagcga gacgcacaaa atcggaaacg gtaaggcgct ccgcgcgcaa
     2581 ggatggatcg atatccgcgt ccttcaaaag tgcggcggtg tcgcccccta gagcagagag
     2641 gcttgatcgc agcatcttcc gtctttggcc gaaggccgct gccgtaagct cctccaaaaa
     2701 tttcacagga acagcgtcct gcggcggggc tgtgggtgtg atctctacca gggccgaaga
     2761 gaccttgggc ggcggggtaa acacggccgg gggcagggta aggagcaggc gggcgcgggc
     2821 gcgatactga gtaagcaccg cgaggcggcc atagtccgcg gtgccggact ccgcaacgaa
     2881 gcggtcggcg acctcgcgct ggaccatgac cacaagtttg tcgaaccagg gcggccaatt
     2941 ctctgcggac aaccagccaa caatgagcgg cgttgcgata ttgtatggga gattggcaac
     3001 aaccctgcgt gcccccgtct cggcggcgat ggcctcatag tcggcctgca acgcgtccat
     3061 ga
//
LOCUS       DATMGX010000522         5396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_147941, whole genome shotgun sequence.
ACCESSION   DATMGX010000522 DATMGX010000000
VERSION     DATMGX010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147941"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1936)
                     /locus_tag="VNR65_08435"
     CDS             complement(<1..1936)
                     /locus_tag="VNR65_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18738.1"
                     /translation="MAETAKVSGPEEASSNTEEPGIGAKKLTEHLAEASSKLIEDSSA
                     ELKASQELEGSNDPQPENLEEKNTDTFAGDVAERRTEAVEIHENDDLDEGEHASDEGE
                     DSSDYENEEGFFTGWDGFDEIHELSSAEELVDDEEQTQPFEGEAGHRTLSEIALASGT
                     LHTLEGSNDSGAGLSSKHDELADAVQSALMSVYGEEPSDAADRRSVPPPPDMHASGPG
                     WASEDSLSPQDVILNYFDYQPAPGNGRGTISDYANAGYNSDTPGVFTQPHDYPPPQYR
                     PQWASSPPALAEYDDPEPYPTGHGKAPKSADTSERESSRLLGAAAIGLVGGIAIAASL
                     AVFVINSYGPGGRAAPGASAQTADPSPAGYGRWGKNGADLEASKAAEVASVPDAAPVL
                     AASDVLVMSGQPSPLAIKVKPEQANEKALISITGIPEGARLNAGVDAGGGNWLLPPRR
                     LDGLTINVPAGAPDAALLGVQVLDSNIRTPLSEKKQFSIRVSAAKAEPAAQTTVVPRT
                     QPEIVTIKPPQPDLASVPETPKPARAAATFFSTETVPASASVMAPQAEPQAKPANANG
                     AGPQTALRQAALQAPAASSDQQKVAPKTEIEELIREGNKRMRDGDILEARQLYQKAVA
                     LGDPEAALAMGRSYDPIYFAR"
     gene            complement(1982..3064)
                     /locus_tag="VNR65_08440"
     CDS             complement(1982..3064)
                     /locus_tag="VNR65_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWJ18739.1"
                     /translation="MAQGPAGPFGPPRGPKAPPPPPAVGTVGVLTGDVDSTGLRIASD
                     LARILDNGNELRIIPMAGKGSIQSITDLTTLKAVDIAIVQSDVLTRFAKMSRRPGFQT
                     HTQYVAKLYSEEFHVLSRMQYLCLQDLTGRRVSFGPKDSGMAITAEIVFEANGIAVNP
                     LYLDHEEAIERLKRGELDALVYVGGKPSRAFDKISHKDKVHFLDVEYLPGLQAAYLPA
                     IMTAEDYPGLVASNESVGTIAVSTVMAVQSSPPQSERFRILSRFTERFFASIDKFKTG
                     SYSPKWREVDIRAPLKGWQRFQPAERWLFANAEPRIEPVAAPGGQQLKTMVKKFVKTQ
                     RGSSANQEELFNKFIRWYQQQNKPQH"
     gene            3345..4100
                     /locus_tag="VNR65_08445"
     CDS             3345..4100
                     /locus_tag="VNR65_08445"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWJ18740.1"
                     /translation="MKFRNKDRKQASASDGVRLYAIGDIHGRLDLLEALLSLIERDQE
                     GRADLPVTLIFLGDYVDRGPDSIGVVERLIRGFSSPYEPVFLKGNHEDLLLSFLNDPI
                     TGPHWLYNGGDAALLSYGIEPETLKLAAWGGPEGLAEVAQQFRAALPEAHLHFYEGLR
                     LSCRIGDYFFTHAGVRPNVPLDQQSEHDLLWIRKEFLNSKEDFGAVVVHGHTPTRAPE
                     DLRNRVGLDTFAVRSGKLTAAGFEGSQRWFLST"
     gene            complement(4135..>5396)
                     /locus_tag="VNR65_08450"
     CDS             complement(4135..>5396)
                     /locus_tag="VNR65_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18741.1"
                     /translation="KAANQLVDNSRLQMQIVALKDQMADLDLQLAERRNAATVLEQTI
                     TKRFPELDREITAVRAQLQERSYELQDFTSKLARRDEELAATQEAAASYQEELQRLHQ
                     VLERSAADRSGRRVRRASQWDLEDYRAEYDRLNLELSKLRQQLVQFQDRDSQQVGVIK
                     GELQKLAELILTSAQTKGGSSQAERIEPSVKRDARRERPVPWRQNAQPPMPLAARLKL
                     NADTQPAAQIPGATPLLASLKADSLSDSAPASGASSLTSPVLTILPEETIFTSAPNNG
                     HAEGSDRPKILQEVVTANDSEEAEPLAGSETKGDAQEEKKPMHAAGNGHGALDEGVSS
                     GDGESGRAESDDPTGFATGNGAHHASHQNGDAAEEHLAEDINLDSLNPDPDDEKIGPH
                     LPTLLERLRSIGEDHAESDKPLSPPRA"
BASE COUNT          996 a         1567 c         1601 g         1232 t
ORIGIN      
        1 tgcgtgcaaa gtagatgggg tcataggaac ggcccatcgc caaggcggct tccggatccc
       61 cgagggcaac agccttctga taaagctggc gcgcttcaag aatgtcgccg tcccgcattc
      121 gcttgttgcc ttcccgaatc aactcctcga tctcggtctt gggcgcgact ttttgctggt
      181 cggacgacgc ggcgggggct tgaagggcgg cttgccgaag agcggtttgc gggcctgcgc
      241 cattcgcgtt tgccggcttc gcttgaggct ccgcctgggg tgccattacg gacgctgagg
      301 cgggcaccgt ttccgtgctg aagaaggttg cagcggccct cgcaggcttc ggtgtctccg
      361 gcacggaagc gaggtccggc tgtggaggct tgatggtcac gatttcaggc tgtgttcgtg
      421 gtacaacggt cgtctgcgcg gcgggctccg cttttgccgc gctcacacgg atggaaaact
      481 gctttttctc tgacaaaggc gttcgaatat tgctgtcgag cacttgtacg ccgaggagag
      541 ccgcatcagg tgcacccgcc ggcacgttga ttgtgagacc atccagccgg cgcggcggca
      601 acagccagtt tccgccgcct gcgtcgacgc cagcattcag tcgcgcacct tccgggatgc
      661 cggtaatgct tataagggct ttctcattcg cttgctccgg cttgaccttg atcgcgagtg
      721 gagaaggctg acccgacatc acgaggacat cggaggcggc aagcacgggt gccgcgtccg
      781 ggaccgatgc cacctcggcg gcttttgacg cctccaaatc tgcgccgttc ttgccccatc
      841 gcccatatcc ggccgggctt ggatccgctg tctgcgccga cgcgccgggc gccgcgcgac
      901 cgcctgggcc gtaggaattt atgacgaaaa ccgccagcga cgctgcaata gcgatgccgc
      961 caaccaggcc gatggcggcc gcgccgagaa gccggctgct ctcgcgctcg gatgtgtctg
     1021 ctgatttcgg agctttacca tggcccgtag gatagggctc aggatcgtca tactcagcaa
     1081 gcgcgggagg cgagcttgcc cattgcggcc gatactgagg cggcgggtag tcatgaggct
     1141 gggtaaaaac gccgggggta tcgctgttgt acccggcatt ggcatagtcc gagatcgttc
     1201 cgcggccgtt ccccggggcc ggctggtaat caaagtaatt caggatcacg tcctgggggg
     1261 acaggctgtc ctcgcttgcc cagccggggc cagaggcgtg catgtccggc ggcggcggta
     1321 cgcttcttct gtcggcagcg tcggatggct cctcgccata gacgctcatc agcgcggatt
     1381 ggacggcatc ggcgagttcg tcatgcttgc tggagagacc cgcgccggag tcgtttgagc
     1441 cttccaatgt gtgaagtgtg ccggaagcga gcgcgatctc tgaaagagtg cgatggcctg
     1501 cttcgccttc gaaaggctga gtctgctcct cgtcatcgac aagctcctca gcggaactca
     1561 gctcatggat ttcgtcgaag ccatcccaac cggtgaagaa accctcctca ttctcataat
     1621 cggagctgtc ttctccctca tcggacgcgt gctctccctc gtctagatcg tcgttttcat
     1681 ggatctccac agcctcggtc cgccgctcag caacgtcgcc ggcgaacgtg tcggtgtttt
     1741 tttcttccag gttttcgggc tgcggatcat tggagccttc gagctcttga gaggctttca
     1801 actcggctga ggagtcctca atcagctttg atgaggcctc tgccagatgc tcggttagtt
     1861 tctttgcgcc aatgcccggt tcttccgtgt tggaagaagc ctcttcgggc ccactgacct
     1921 tggctgtctc cgccattttc tgctcctgtc gggcatctcg catcactgga cctgccggct
     1981 gctaatgctg cggcttgttc tgttgctgat accagcggat gaacttgttg aagagctcct
     2041 cctggttagc ggagcttccg cgctgagtct tgacaaattt tttgaccatc gttttcagct
     2101 gttggccgcc gggtgctgcc acgggctcta tccgaggctc ggcattcgca aacagccatc
     2161 tctcggcggg ttgaaatcgc tgccagccct tgagcggcgc tcttatatcg acttcacgcc
     2221 acttcggact gtagctgccc gttttgaatt tatcgatgct cgcgaaaaat cgctcggtaa
     2281 atcgagatag aatacgaaaa cgttcggatt gaggaggcga agactggacc gccatgacgg
     2341 tggagacggc gatggtcccc acgctttcgt tcgacgcaac aagccccggg taatcttccg
     2401 cggtcatgat cgccggcaga taagcggcct gaagaccggg caagtattcg acatccagga
     2461 aatgaacctt gtccttgtgg ctaatcttgt cgaaagcccg cgatggcttg ccgccaacat
     2521 agacgagcgc atccagttcg ccgcgcttga gccgctcaat ggcctcttca tggtcgagat
     2581 aaagcgggtt gaccgcgatt ccgttggcct cgaaaacgat ttcggctgta atcgccatac
     2641 cgctgtcctt cgggccaaag ctcacccggc ggcccgtcaa atcctgcaga cacagatatt
     2701 gcatccgcga taggacgtga aattcttcgg aataaagctt cgccacatat tgcgtgtgcg
     2761 tttggaagcc gggccttctg ctcatcttgg caaagcgtgt cagaacatcg gattggacga
     2821 tggcaatatc gacggccttg agggtcgtaa gatcggtgat gctttgtatg gatccttttc
     2881 cggccattgg aatgatccgc agctcatttc cgttgtctag gatgcgagcg agatcactgg
     2941 cgattcgcaa tccggtgctg tcgacgtcac cggtaagcac gcccacagtt ccgaccgccg
     3001 gcggcggggg aggtgccttt gggcctcgag gcgggccaaa aggcccggcc gggccttgag
     3061 ccatcgtgct gccgatcgta gaaaaaagga taaaaaggct tatggcgaga agcctgacca
     3121 ctattcatca cccttcaatt gatgacggac gccaaaccga atggcggcag cggataagat
     3181 accatcgaag gcaaaagatt cgaaacgcgc ggactaccaa aaagaagtac gtttgcgacg
     3241 tttgatgact gagagaagaa agcggcgaaa ctgtgccacc gggcggtgtc tcgctcagcg
     3301 acccggcagg caaatcgtag cggatccgcg ggagaaaaat tcctttgaaa ttcaggaaca
     3361 aggatcggaa acaggcaagc gcgtcggacg gtgtccggct ttacgccatt ggagacatcc
     3421 atgggcgcct ggatctgctt gaggccttgc tgagcctgat cgaacgggac caggaaggcc
     3481 gcgctgacct gccggtcact ctcatctttc tcggagacta cgttgatcgc ggccctgatt
     3541 cgattggcgt cgtcgagcgg ttgatccgcg gtttctcgtc gccatacgag ccggtatttc
     3601 tcaaaggaaa ccacgaggat ttgcttctct ctttcctcaa cgatccgatc actggcccgc
     3661 attggctcta caatggcgga gacgctgcac ttctgtctta cggtatcgaa cccgagacac
     3721 ttaaactcgc ggcatggggc ggtccggaag ggttggccga ggtggctcag caattccgtg
     3781 ctgcgctgcc agaggctcat cttcatttct acgaagggtt gaggctctcc tgccgcatcg
     3841 gggactactt tttcacccac gctggggtca ggcccaatgt tccgctcgat cagcaaagcg
     3901 aacatgacct cctttggatc aggaaggaat ttctgaactc gaaagaagat ttcggggcgg
     3961 tcgtcgtcca tggtcacaca cccactcgcg cccctgaaga ccttcgcaac agggtaggat
     4021 tggacacttt cgcagttcgc tcaggcaagc tcacggcggc cggtttcgaa ggctctcagc
     4081 gctggttctt atcaacttag atcaacccca catcaaatgg atgaggactg ctctctaggc
     4141 tcgcggaggc gacaatggct tgtcgctctc ggcatgatct tccccaatgc tccgcagtct
     4201 ttcaaggagt gttgggaggt gcgggccaat tttttcgtcg tcgggatcgg ggttcaagga
     4261 atcgagattg atatcctccg ccaaatgttc ctctgccgcg tcaccgttct ggtgggaggc
     4321 atggtgcgcg ccatttcccg ttgcaaatcc ggtggggtca tcagactcgg cacgcccgct
     4381 ctctccatcg ccagaagaca caccctcgtc taaggcgcca tgtccgttcc cggcggcgtg
     4441 catcggcttc ttttcctctt gagcatctcc ttttgtttcg ctgcctgcga gaggctccgc
     4501 ctcctctgag tcgttcgccg tcaccacttc ctgcaaaatc tttggccgat cgcttccttc
     4561 tgcgtgacca ttattcggtg cggaggtgaa aattgtttcc tccggaagga ttgtcagcac
     4621 gggggatgtc aggctcgagg ctcctgaggc cggcgccgaa tccgaaagac tatctgcttt
     4681 cagggaagcc aggagtgggg ttgcacccgg gatctgcgcc gcgggctgtg tgtcggcgtt
     4741 gagcttcagt cttgcggcga gcggcatggg tggttgcgca ttttgacgcc acggcaccgg
     4801 cctttctctg cgtgcatcac gctttaccga aggctcgatg cgctctgctt gcgaggatcc
     4861 gccttttgtc tgcgccgatg taaggatcaa ctcggcaagc ttctgcaact cgcctttgat
     4921 aacgccaacc tgctgagaat cgcgatcctg aaactgcaca agctgctgac gcagcttcga
     4981 cagctcgaga ttcagacggt catactcggc ccggtaatct tccagatccc attgagaggc
     5041 ccggcgaaca cgtcgtccac tccggtcagc ggcgctcctt tccagaactt ggtgcaggcg
     5101 ttgcagctct tcctgataac tggcggcggc ctcctgcgtg gcggcaagct cttcatcacg
     5161 gcgggcgagc ttgctcgtaa aatcctggag ttcgtaagac cgttcctgaa gttgcgccct
     5221 aacggcggtg atttcgcggt caagctcggg aaagcgcttg gttatggttt gttcgagaac
     5281 ggtggccgcg ttgcgccttt cggccagctg gaggtcaagg tccgccatct gatccttcag
     5341 ggccacaatc tgcatttgca ggcggctgtt gtccacaagt tgattcgcgg ccttct
//
LOCUS       DATMGX010000523         2728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_314367, whole genome shotgun sequence.
ACCESSION   DATMGX010000523 DATMGX010000000
VERSION     DATMGX010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314367"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VNR65_08455"
     CDS             <1..900
                     /locus_tag="VNR65_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HWJ18742.1"
                     /translation="AHDTVSFLYGVKKALPGWRVTEGASLDAAREADAVLLCIGESAS
                     ICGEATSRARPGIPDEQRKAAADVFSLGKPVIVALTSGRPLIDPWLFEKADAVLATWF
                     LGSEAGAALGGILTGAVNPSGKLPVTWPYDPGQIPIFYAQRPTGRPAKTDEHDTSKYL
                     DVPIGPQFPFGHGLSFTRFAYGTPRASSETIRRGDHFFVEAEVTNTGDVAGDETAFLF
                     IRDPLASVARPLLELKGFAKISLDPGTTGVVRFELSADDLSFPGVEGEPCLEAGAVQI
                     FVGPSANRDLLKSCTVLVAQDSA"
     gene            complement(908..1330)
                     /locus_tag="VNR65_08460"
     CDS             complement(908..1330)
                     /locus_tag="VNR65_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWJ18743.1"
                     /translation="MAPAVHADMQSGVVSLGGAKLPADIQYKGLPGAPQIATLFGDPA
                     KPGIFVQRVKFPSGIKIMPHFHPDEPRTIVVLSGTLYFGLGDTFDENKLQAFPAGTFF
                     TEAPKVSHFVWAKDGDVFVQLTSVGPTANISVKTVAKE"
     gene            1329..1472
                     /locus_tag="VNR65_08465"
     CDS             1329..1472
                     /locus_tag="VNR65_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18744.1"
                     /translation="MSKKDNAAAIPSAFEPIPNAAEADLVMFPLRFIARHKAKTKLNI
                     PKY"
     gene            1595..>2728
                     /locus_tag="VNR65_08470"
     CDS             1595..>2728
                     /locus_tag="VNR65_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ18745.1"
                     /translation="MLAQSQFSTLLSGIYDAATDFSRWEEVLRSLASTLGGNGAVLGI
                     SGEGQRFSAYVAPLTEPAFLTSYNAYYHKVSPLMPRIKALPAGSVATDRMVLPRSEFE
                     TSEIYNDWILPQGLCNKLYTVLSSDPNQRIMIGVHRTREFHEDQIELCRLLAPHLQRA
                     MAISLKMRLLEGERGGFAEILDHLDQAVILLDAEARPLFVNKAAETLFAPGRAFRLMS
                     GQILALRAADTLRLHQLVASCGGEAIAKNAGGQVLLCGLDGGAPLPAVIIPTRMRICW
                     LLPHQPVAMLFVRSSPQRSLPQPEELRARYGLTHAEAALAVEICAGDGLQAAANRRGV
                     SLATARTHLAHIFQKTGVGRQAELVRLIMQVGEGGNLGPPKNGA"
BASE COUNT          529 a          806 c          813 g          580 t
ORIGIN      
        1 gcacatgata ctgtgagctt tctgtatggc gtcaagaaag cgcttcccgg ctggcgggtt
       61 acagaaggcg cgtcgctcga cgcggcccgc gaagccgacg ccgtcctgct ttgtatcggc
      121 gaaagcgcga gcatttgcgg agaggcgaca agccgcgcac ggcctggcat tcccgacgag
      181 cagcgcaagg ctgccgcgga tgttttcagc ctcggaaagc cggtgatcgt ggcgctgaca
      241 tccggccggc cgctcatcga tccttggctt ttcgaaaagg cggacgccgt tctcgcgacg
      301 tggtttctcg gctcggaggc gggcgctgca ttgggcggca ttctcaccgg agccgtgaat
      361 cccagcggaa agcttcctgt gacctggcca tatgatcccg gccagatccc gattttttat
      421 gctcagcgcc caactgggcg gccggccaag accgatgagc atgatacgag caaatatctc
      481 gatgttccca ttggacctca gtttcctttc ggccatggcc tgtcgttcac gcgctttgcc
      541 tatgggacgc cgcgagcatc ctcagagacg atccggcggg gcgatcactt cttcgtcgag
      601 gccgaggtca cgaacacggg cgatgtcgcg ggcgatgaga cggcttttct gttcatccgc
      661 gatccccttg cttcggtggc gcggcctctc ctggaactca aaggctttgc caagatcagc
      721 ctcgaccctg gaacgacagg cgttgtgcgg ttcgagctga gcgcggacga cctgagcttt
      781 cccggcgtcg aaggcgagcc ctgcctcgaa gctggcgcgg tgcagatttt cgtcggtccc
      841 agcgccaacc gggacttgct caagagctgc accgttctcg tcgcccagga ctcggcctag
      901 ccgcgcccta ttccttagcg acggtcttca cagagatgtt ggcagtcggg ccaacgcttg
      961 tcagttgcac gaacacgtcg ccgtccttgg cccagacaaa atgggagacc ttcggagctt
     1021 ccgtgaagaa ggttccggcc gggaacgctt ggagcttgtt ttcatcgaag gtgtcgccca
     1081 ggccaaaata aagcgtgcct gacagcacga caatcgtccg cggttcatcc gggtgaaaat
     1141 gcggcatgat cttgatgccc gacgggaatt tgacccgctg gacaaaaatg ccgggcttgg
     1201 ccggatcgcc aaagagagtc gcaatctggg gcgcgcccgg aagccctttg tattgaatgt
     1261 cggcgggaag tttggcgcca cctaacgaaa cgacgccgct ttgcatatcg gcatgaacgg
     1321 ctggggccat gagtaagaaa gacaacgccg ctgccattcc aagtgctttt gaacctattc
     1381 cgaacgctgc cgaggctgat ttagtcatgt ttcctctccg ttttattgca agacataaag
     1441 caaagaccaa gctcaatatc ccgaaatatt gaatgacaag cataacggat cgatacggcg
     1501 cggcatcagc cgttcgtttg acttttctat ttttttcatt agcctttgga aacgaggggc
     1561 agatggccgg cggtgcagaa catcggaaaa cgccatgctt gcccagtcgc agttttctac
     1621 gctcctttcc gggatatacg acgccgctac ggacttctcg cgatgggaag aggtgcttcg
     1681 cagtctcgcg tcaacgctcg gcggcaacgg cgcagtgctc ggtatcagcg gagaaggcca
     1741 gcgtttctcg gcgtacgtcg ctcccctgac agagcctgct ttcctgacaa gctataacgc
     1801 ttactaccat aaggtctccc cgctgatgcc acggatcaaa gcgcttccgg caggaagcgt
     1861 cgcaaccgac cggatggtcc ttccccggag cgagtttgaa acaagcgaga tttataacga
     1921 ttggattctg ccccaggggc tttgcaacaa gctctatacg gtcctttcga gcgatcccaa
     1981 tcagcggatc atgatcgggg tgcaccgcac ccgggagttt cacgaggatc agatcgaact
     2041 ctgccgcttg cttgctcccc atcttcagcg cgccatggcg atcagcttga agatgcgcct
     2101 tttggaaggc gagcgtggcg gctttgctga aatcctggat caccttgatc aggctgtgat
     2161 cctgcttgac gccgaggccc ggcctttgtt cgtcaacaag gcagctgaaa cactctttgc
     2221 gccgggccgc gcatttcggc tcatgagtgg tcaaatctta gcgcttcggg ccgccgatac
     2281 cctgagattg catcaattgg tggcttcttg cggcggtgag gcaattgcaa agaacgctgg
     2341 cggccaggtc ctcttgtgtg gcctggatgg cggcgcgcct ttgcctgccg ttatcatacc
     2401 aacgcgcatg aggatttgct ggttgctacc ccaccagccg gttgcgatgc tctttgtgag
     2461 atcctcacct cagcgctcct tgccgcagcc ggaagagctt cgggctcgat acgggctgac
     2521 ccatgcggag gcggccctgg ccgtagagat ctgcgccggc gacggccttc aggccgcagc
     2581 gaacaggcga ggcgtcagcc tggcgaccgc acgcacgcat ttggcccaca ttttccagaa
     2641 gactggcgtt ggacgtcagg ccgaacttgt ccgcttgatc atgcaggtag gcgagggcgg
     2701 caatctgggg cccccgaaaa acggcgct
//
LOCUS       DATMGX010000524         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_401018, whole genome shotgun sequence.
ACCESSION   DATMGX010000524 DATMGX010000000
VERSION     DATMGX010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401018"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VNR65_08475"
     CDS             complement(<1..465)
                     /locus_tag="VNR65_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18746.1"
                     /translation="MVGIAPSQILLALSFAALLGCGEKLRLPRIRLPLALFLLGTVIS
                     LLLSDDPAAGLPQIRKFYVFLELLVVFSLLRSLAMVRLLFLTWAGIGALTAVRGCVQF
                     AGKVQEAHRLGRNFYEFYVGERITGFTSHWNTYSAEEMFALIMLASFLFFAPA"
     gene            616..1797
                     /locus_tag="VNR65_08480"
     CDS             616..1797
                     /locus_tag="VNR65_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ18747.1"
                     /translation="MRRILSLALLFAGALRADTVLVLSFFNHAKSANLDWVGESIGQS
                     VRDSLASEGVLVLAREDRLEAYRRLSLRPGAELTHASIIKIGESLDASQVIYGFYELL
                     PSEPGNPQSKGSLRITARILDLKHTRQGAAYSELGALEDLGSLAVHLGWQTLEWLNPK
                     AAPGEQEFMKARPAVRLDAVESYVRGLLSTSPEQRHRYFTQAARLDEHYSQPCFELGR
                     IFWEKKDYKVAAGWLERVAHSDPHYLEAQFFLGLCRFANGDFKGAEQSFQLVAAAIPL
                     NEVYNDLGVVQAQRQDYAAAAASFRKALEGDDADPDYHFNLGAALWRSGQYSDAADSF
                     RAVLARNSADSEATTLLGRTLKKEAPRPGDTNPDGKPRLKTNYEESAFRQLQAELGIK
                     K"
     gene            complement(1843..>2032)
                     /locus_tag="VNR65_08485"
     CDS             complement(1843..>2032)
                     /locus_tag="VNR65_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18748.1"
                     /translation="PDQKLRIAINSYRAGGSGGYSMLHGAKVLWRSSEGIRELVIRYY
                     TERKSIQGETAGNWKIVQ"
BASE COUNT          414 a          671 c          610 g          337 t
ORIGIN      
        1 cgccggggca aagaagagga aggatgccag catgatcagg gcaaacatct cttccgcgga
       61 ataggtattc cagtggctgg tgaagccggt aatccgctcg ccgacgtaaa actcgtagaa
      121 attgcggccg aggcgatgcg cctcctgcac cttgccggca aactggacgc atccgcggac
      181 ggcggtgagc gcgccgattc ccgcccaagt gaggaagagg aggcgcacca tggcgaggct
      241 gcgcagcagc gaaaacacga ccagcagttc gaggaagacg tagaacttgc gaatttgcgg
      301 aagcccggcg gcgggatcgt ccgacaacaa cagggaaatc acggttccga gcaggaaaag
      361 cgcgagcggc agccggatgc gcggcagacg caacttctcg ccacacccca gcagcgccgc
      421 aaaggagagc gccagcagga tctgcgaagg cgcaattccc accatgatcc cggctgcgga
      481 tccaaacgcc agccagcgcg cacttctcac caaaatcggt tcctgagggt tcatgcggga
      541 tcgaactctc agtatggcag agagtggatg gcaaaaccct gcctcctccc gagcccgctg
      601 ttagactgaa ggtacatgcg gcgcatcctg tcccttgcac tactcttcgc cggcgcgttg
      661 cgagccgaca cggtgctggt gctttccttt ttcaatcacg ccaaatcggc caacctcgat
      721 tgggtcggcg agagcatcgg tcagagtgtg cgcgattcgc tggcctccga aggcgtcctg
      781 gtgctggcac gcgaagaccg gctggaggcg tatcggcggc tctcgctccg tcccggcgcc
      841 gaactgaccc acgcatcgat catcaaaatc ggcgaatcgc tcgacgcgtc gcaggtaatc
      901 tacggtttct acgaactgct gccgtccgaa cccgggaatc cgcaatccaa aggttcgctg
      961 cgcatcacgg cgcgcattct ggacctcaaa cacacccggc aaggagcggc ctatagcgaa
     1021 ctcggcgcgc tcgaagacct gggctcgctg gccgtccacc tcggctggca gacgctcgaa
     1081 tggctcaatc cgaaggccgc tcccggcgaa caggagttca tgaaggcgcg tccggcggtg
     1141 cgcctggatg ccgtcgaaag ctacgtgcgc ggcctgctct ccacttcgcc cgagcagcgc
     1201 caccgctact ttacgcaagc cgcccggctg gacgagcact actcgcagcc ttgcttcgag
     1261 ctcggccgga ttttctggga aaagaaagac tacaaggtgg ctgccggatg gctcgagcgc
     1321 gtggcccatt ccgacccgca ctacctggaa gcccagtttt tcctcggctt gtgccgcttc
     1381 gccaacggcg atttcaaggg cgccgaacaa tccttccaac tggtggcggc cgccatcccc
     1441 ctcaacgagg tctataacga tctgggcgtc gtgcaggccc agcgccagga ttacgcggcg
     1501 gcggcggcca gctttcgcaa ggcgctggag ggcgacgacg ccgacccgga ctaccacttc
     1561 aatctgggcg ccgcgctctg gcgctccgga cagtactccg acgccgccga tagcttccgc
     1621 gccgtgctgg cgcgcaactc ggccgacagc gaagctacca ctctgctggg ccgcacgctg
     1681 aaaaaggaag ctccgcgccc cggcgatacc aaccccgacg gcaagccgcg tctcaagacg
     1741 aattacgaag agtccgcttt ccggcagttg caggccgaac tgggcatcaa gaaataggtg
     1801 gcataaagcc tcgccatccg agcgaagccc ggatgtggcc cttcactgca ctatcttcca
     1861 gtttcccgcc gtctcgccct gaatcgactt gcgctccgtg taatagcgaa tcaccagttc
     1921 ccggatgccc tcactggagc gccacagcac cttcgcgccg tggagcatgg agtatccgcc
     1981 gctgccaccg gcgcgataac tattgatggc gatgcgcagc ttctggtccg gc
//
LOCUS       DATMGX010000525         4963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_352233, whole genome shotgun sequence.
ACCESSION   DATMGX010000525 DATMGX010000000
VERSION     DATMGX010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352233"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VNR65_08490"
     CDS             <1..241
                     /locus_tag="VNR65_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWJ18749.1"
                     /translation="NGALFTEEGGIAHEPALKVLAVDTNGAGDVWHGALAVALLEGQE
                     PRKAVRFANAAASIKCSRGGGRLAIPARSEVEAFL"
     gene            complement(299..988)
                     /locus_tag="VNR65_08495"
     CDS             complement(299..988)
                     /locus_tag="VNR65_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWJ18750.1"
                     /translation="MMESVQPFNAGAIEERYLSIKERVWRAAQRHGREPASVKLVAVT
                     KTFEDGEIVPVLRQGHCCFGENRVQEAMRKWPSLRERFPAIELHLIGPLQSNKTKEAV
                     GLFDCIETLDRPKLAEALASEIQRQGKAPKLLIQVNIGLERQKAGIAPADTAAFARRC
                     ATEFGLDINGLMCIPPFEDDPLPHFEKLAALRDELGLPELSMGMSSDFEEAIGAGATM
                     VRVGSAIFGTR"
     gene            1076..3688
                     /gene="leuS"
                     /locus_tag="VNR65_08500"
     CDS             1076..3688
                     /gene="leuS"
                     /locus_tag="VNR65_08500"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWJ18751.1"
                     /translation="MAVERYNAPEREKHWQRIWEEQQTFLTSTDRDKPKYYVLEMFPY
                     PSGRIHVGHVRNYVMGDVVARYKRAKGFSVLHPFGWDAFGLPAENAAMQQKINPREWT
                     YQNIEVMRAQLKRIGLSLDWSREFATCDPEYYHQEQRLFQAFYKAGLVYRKKSKVNWD
                     PVDHTVLANEQVIDGRGWRSGALVEQKELAQWFFKITHYADDLLNGLDTLDKWPEKVR
                     VMQRNWIGKSQGLLMRFNLSGEKLPDGFRNVEVFTTRPDTIFGASFIGISPEHPLAQE
                     LAKSDPKLQDFIEECRRIGTAQEEIDRAEKKGYDTGIVAAHPFRSTMKLPVYVANFIL
                     MEYGTGAIFGCPAHDQRDLDFARKYRLKVKAVVIPEDADPAEFKVRNEAYTGPGRLAN
                     SSFLNGMSVEDAKEEVAQRLAKRNMAEKKTTFRLRDWGVSRQRYWGCPIPMIHCEKCG
                     VVPVPDKDLPVVLPHDVSFDKPGNPLDLHPTWKHVECPSCGGKATRETDTCDTFVDSS
                     WYFARFCSPHSKEPVEKTAVDYWLPVDQYIGGVEHAILHLLYARFFTRAMKDCGYLTL
                     SEPFAALFTQGMVLHETYFDDKQHWLAPADVRIEAEDGVRKAFRLDGNREPIQIGGVE
                     KMSKSKKNTIDPDIFTEHFGADTARWFMLSDSPPERDVLWTEEGAQGAWRFIQRAWRL
                     LNEGLPLLPARNGNNPVSFSAPAQALRRAAHKGLNAVAEEIEGLRYNRAIAAVYEFAN
                     ALTASLQDKAAREDASFGFAMREALVFFVHMMNPIMPHLAEEGWALLGYNTLLANESW
                     PEVERSLLIDDTVTIAVQVNGKRRDEIVVPRNADRAVLEAAALRLESVTRAVDGRPIK
                     KVIVVPERIVNVVA"
     gene            3675..4238
                     /gene="lptE"
                     /locus_tag="VNR65_08505"
     CDS             3675..4238
                     /gene="lptE"
                     /locus_tag="VNR65_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWJ18752.1"
                     /translation="MSWLEHSKDSSRLLKGAAVAVITSVLGLGVTGCAFHPMYATTST
                     GGNLTDAMKAVDIAPIPSRVGQRLRNELIFGTTGGGEASAPVYRLDIALRESVRNTLV
                     TNTGAPTGQILQLDAEFRVVRIKDNEALFKGYSTSEAAYDLTGFSGTTGSIYGDTRAV
                     LDAENRAARSLADTLKTRVAAFLSRDA"
     gene            4270..>4963
                     /locus_tag="VNR65_08510"
     CDS             4270..>4963
                     /locus_tag="VNR65_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18753.1"
                     /translation="MVAPSAASAHKFLASPPHAVRGFLFYGSDSLQISARAEALAAVL
                     AKKAGPDAELIRLHDTDAAAVPDRIATELKTGSLFGGTKIIWLTSLPAKAHAAVLEAV
                     ATPIEGAFLIAQAPDLKKSHKLAQVFEGAAYLASVACYGEDRDSLIAGIREHVSSFGC
                     EIDGDAAALVAARCDYSALLARSEAEKLITYAGPARRITSADVDDCLIDQQTAGLAEI
                     IDHALDGDGRKAI"
BASE COUNT         1016 a         1443 c         1492 g         1012 t
ORIGIN      
        1 aaacggtgct ttgtttactg aagagggcgg tatcgcgcat gagcctgcat taaaagttct
       61 cgcggtcgac acgaatggag cgggtgatgt ctggcacgga gcgcttgctg ttgccctctt
      121 ggaagggcaa gagccgcgga aggcggtgcg ttttgcgaat gccgccgctt ccataaaatg
      181 ttcccgaggg ggcgggcgct tagccatccc cgcccgaagt gaagtggagg cattccttta
      241 gagctggcaa aaaccagcgg caatgcgaca cttgcaaaat gcgctatcct gaccgctttc
      301 accgcgtgcc aaaaatcgcg gacccgacgc ggaccatggt ggcgcccgcg ccgattgcct
      361 cctcgaagtc gctgctcatc cccatgctta actcgggtag tccgagttca tcgcgaagag
      421 ccgccaattt ttcaaaatga ggcagagggt cgtcctcgaa aggcggtatg cacatcaagc
      481 cgttgatatc gagaccaaat tcggttgcgc accgcctcgc aaacgctgcc gtgtcggccg
      541 gggcgatccc agctttttgc cgctcaagac cgatgttgac ctgaataaga agcttgggcg
      601 cctttccctg ccgctggatt tcagaggcca acgcctcagc cagcttgggc ctatccagag
      661 tttcgatgca atcgaacaag ccgaccgctt ccttcgtctt attgctttga agcgggccga
      721 tcaaatgcaa ctcaatcgcg gggaaacgtt cgcgcaagct gggccacttc cgcatcgctt
      781 cctgaacgcg gttctcgccg aagcaacagt gcccttggcg aagcaccggc acaatttcgc
      841 cgtcctcgaa tgtcttcgtc accgctacca gcttgaccga agcgggctcg cgcccatgcc
      901 gctgcgcggc gcgccacacc cgctccttga tcgagagata gcgctcctcg attgcacctg
      961 cattgaacgg ttgaacactt tccatcattt ggcgagcttc tcttgaccgg ggcggacttt
     1021 caatttcata tcacgcaaac ctttttgccg cacttggtgc gcaggatatc caatcatggc
     1081 agtagaacgc tacaacgctc cggagcgtga aaaacattgg cagaggattt gggaggagca
     1141 acagactttt ctcacctcca cggatcgtga caagccaaag tattacgtgc ttgagatgtt
     1201 tccctatccg tcggggcgca tccatgtcgg gcatgtgcgc aattatgtga tgggcgatgt
     1261 cgtggcgcgc tacaagcggg cgaagggctt cagcgtgctg cacccgtttg gatgggacgc
     1321 tttcggcctt cccgccgaaa acgcggcgat gcagcagaag atcaatccgc gcgaatggac
     1381 gtaccaaaac atcgaggtga tgcgcgcgca gctgaagcgc atcggcctgt cgctggattg
     1441 gtcgcgcgag ttcgcaacct gcgatcccga atactatcac caggaacagc ggctgtttca
     1501 ggctttctat aaggcgggcc tcgtctatcg gaagaagagc aaggtgaatt gggacccggt
     1561 cgatcatacg gtgctcgcca acgagcaggt gatcgacggc aggggctggc ggtccggcgc
     1621 gctcgtcgag cagaaagagc tggcccagtg gtttttcaag atcacgcact acgccgatga
     1681 cctgctcaac ggtttggaca cgttggacaa gtggcccgag aaagtccgcg tcatgcagcg
     1741 gaactggatc ggcaagagcc agggcctctt gatgcggttc aacctgtcgg gcgagaagct
     1801 gccggacgga ttccggaatg tcgaggtgtt caccacgcgt ccggacacga tctttggcgc
     1861 aagctttatc ggcatttcgc ccgagcatcc gctggcgcag gagctggcga aatccgatcc
     1921 gaaattgcag gatttcatcg aggagtgccg ccgcatcgga acggcgcagg aggaaatcga
     1981 ccgcgccgag aagaagggct acgacaccgg cattgtggcg gcgcatccgt tccgttcgac
     2041 catgaagctg ccggtttacg tcgcgaactt cattttgatg gagtacggca ccggcgcgat
     2101 tttcggctgc ccggcgcatg atcagcgcga cctcgatttc gcgcggaaat atcgtctgaa
     2161 ggtgaaggct gtcgtcattc cggaagacgc agatccggcc gaattcaagg tcagaaacga
     2221 ggcttacacc ggccctggcc gcctggcgaa ctcatcgttt ttgaacggca tgtccgtcga
     2281 ggacgcaaag gaggaagttg cgcagcggct cgccaaacgc aacatggccg agaagaagac
     2341 aacctttcgg ctgcgcgact ggggtgtttc ccgccagcga tactggggtt gtccgatccc
     2401 gatgatccac tgcgagaagt gcggggtcgt tccggtgccg gacaaggatt tgcccgtggt
     2461 gctaccgcac gatgtctcgt tcgataagcc ggggaatcct ctcgacctgc atcccacctg
     2521 gaagcacgtg gagtgtcctt cctgcggcgg aaaagcgacg cgcgaaaccg acacctgcga
     2581 caccttcgtc gacagctcct ggtattttgc acgtttctgt tcaccgcatt caaaggagcc
     2641 cgtcgagaag acggcggtcg attattggct gcccgtcgat caatatatcg gcggcgtcga
     2701 gcatgcgatc ctgcatctgc tctatgctcg cttcttcact cgcgccatga aggattgcgg
     2761 ctatctcact ctgagcgagc cattcgcggc gctctttacg cagggcatgg tgctgcacga
     2821 aacctacttc gacgacaagc agcattggct tgcgccagcc gatgtgcgca ttgaggcgga
     2881 ggatggcgtt cgcaaagcct tccggttgga tgggaaccgt gagccgatcc agatcggcgg
     2941 cgttgagaag atgtcgaagt cgaagaagaa cacgatcgac ccagacatct tcaccgagca
     3001 tttcggcgcg gacaccgccc ggtggttcat gctgtcggac agtccgccgg agcgcgacgt
     3061 gctctggacg gaggaaggcg cgcaaggtgc atggcgcttt attcagcgcg cctggagact
     3121 cctgaacgaa ggtttaccgc tgcttccggc ccgcaatggg aacaacccgg tttccttctc
     3181 ggctcccgcg caggcgctgc gccgggcggc acataaaggt ttgaatgccg tcgcagaaga
     3241 gatcgagggc ctgcgctaca atcgcgcgat tgccgccgtg tacgagtttg cgaacgcgct
     3301 caccgcatcg ttgcaggaca aagcggccag agaggatgcg tcgttcggct tcgccatgcg
     3361 cgaagcgctc gtcttcttcg tgcacatgat gaatccgatc atgcctcacc tcgcagaaga
     3421 aggctgggcg ctgctcggct acaacacctt gctggcaaac gagtcctggc cggaggttga
     3481 gcggagcttg ctgattgacg ataccgtgac cattgccgta caggtaaacg gcaagcgtcg
     3541 tgatgagatc gtcgttcccc gcaatgcgga tcgagccgta ttggaagcgg cggctcttcg
     3601 ccttgaaagc gtcacgcgcg cagttgatgg tcggcccatc aagaaggtga ttgtggtccc
     3661 cgaaaggatc gtcaatgtcg tggcttgaac actcgaaaga cagcagccgc ctcctcaagg
     3721 gggccgcggt tgctgttatt acgagtgtgc tgggtctcgg tgtcaccggc tgcgctttcc
     3781 atccgatgta tgcgacgacg tccaccggag ggaacctcac cgatgctatg aaggccgtgg
     3841 atatcgctcc gattcctagc cgcgtgggac agcgtttgcg caatgagctc atatttggca
     3901 cgaccggcgg aggggaagcg tcagctccgg tttatcggtt ggacatcgcg cttcgcgaaa
     3961 gcgtgagaaa tacgctcgtc accaatacgg gtgctccgac cggacaaatc ctgcagctcg
     4021 atgcggagtt ccgcgttgtc cggatcaaag ataacgaagc tctgttcaag ggctacagta
     4081 cctctgaagc ggcctacgac ctcaccggat tttctggaac caccggctcg atctatggcg
     4141 acacccgcgc ggtgcttgac gcggaaaacc gtgcggcccg ctcgcttgcc gataccttga
     4201 agacgagggt cgccgctttc ctttctcgtg acgcttaatc gggactgagt tttcttcctc
     4261 ggaaatggaa tggttgcgcc aagcgcagca tcagctcaca agtttctagc ttcgccaccc
     4321 cacgcggtgc gcggcttttt attctatgga tcggacagtc ttcagatctc agcaagagcg
     4381 gaagctcttg cggctgtgct cgcgaagaaa gccggcccgg atgcggaatt gatccggctt
     4441 cacgataccg acgccgccgc cgttccagac cggattgcga ccgaattgaa gacaggatcg
     4501 ctgttcggcg gcaccaagat catctggctg acctccctgc cagcgaaagc gcacgcagct
     4561 gtgctggaag ccgttgcgac gccgattgaa ggcgcctttc tgatcgcgca agcgcccgac
     4621 ctcaagaaaa gccataagct ggctcaagtc tttgaaggcg ccgcttatct cgcctccgtc
     4681 gcctgttatg gcgaggaccg cgatagcctc atagccggca tccgagagca tgtttcatcc
     4741 ttcgggtgcg agatcgacgg cgatgccgcc gcgcttgttg cggcgcgctg cgattattct
     4801 gctctgctcg cgcggagcga agccgaaaag ctgatcacgt acgccggtcc ggcacgccgg
     4861 attacatccg ccgacgttga tgactgcctg atcgatcagc agactgcagg cctcgccgaa
     4921 atcattgatc acgccctcga tggagacggg cggaaggcga tcg
//
LOCUS       DATMGX010000526         2545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_290972, whole genome shotgun sequence.
ACCESSION   DATMGX010000526 DATMGX010000000
VERSION     DATMGX010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290972"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VNR65_08515"
     CDS             <1..771
                     /locus_tag="VNR65_08515"
                     /inference="COORDINATES: protein motif:HMM:NF036542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HWJ18754.1"
                     /translation="EREEQGDKLRVRAELFADHYSQARLFWKSMTESEQAHIASSFTF
                     ELSKVVLEHVPSRMIANLRNVDEELAERIAAGIGIDLPRKADAQTKVLDLDPSPALSI
                     QKNMKKTLQGRKVGILYADGSDGAEIKAVQKAIEKEGATAFLVAPKVGGAKLKGGTVL
                     RADGQLAGSPSQLFDAVAIVLSDQGCETLMNEAGALQFVMDAFGHLKAIGASKAAKPL
                     LDKAGVAPDDGVTGLGKDFIEAARMRFWDREPSLRKLA"
     gene            1033..1470
                     /locus_tag="VNR65_08520"
     CDS             1033..1470
                     /locus_tag="VNR65_08520"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HWJ18755.1"
                     /translation="MTALLGMLALAGYQNRDKISEMLRGATASGSAPGTASQSSPLGG
                     LLGNLGGMLGGAGAGGLLSGGLGELLTKFQANGHGDTARSWIDNGPNQEISPPDLKQA
                     IGPDVLAALEQKTGLSQEELLARLSRELPNAVDKYTPDGRLPH"
     gene            1504..1764
                     /locus_tag="VNR65_08525"
     CDS             1504..1764
                     /locus_tag="VNR65_08525"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWJ18756.1"
                     /translation="MGILWTIIIGFVAGVIAKFIMPGDNEPSGFILTAVLGIIGAFVA
                     TYLGQALGWYGPGQGAGLIGAVVGAVIVLLVYGMVAGRSRAI"
     gene            1791..2225
                     /locus_tag="VNR65_08530"
     CDS             1791..2225
                     /locus_tag="VNR65_08530"
                     /inference="COORDINATES: protein motif:HMM:NF023621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HWJ18757.1"
                     /translation="MGIFGKIMGAIFGSHAEAATPSGGTSSTATGGAPPTSSTSGTAK
                     ATPMGAGGASPGPGTVDVAAILDKAVAGKGEKLAWKTSIVDLMKALDIDSSLTARKDL
                     AKELGYTGDTSDSATMNIWLHKKMMEELAANGGKLPADIKKS"
BASE COUNT          554 a          721 c          790 g          480 t
ORIGIN      
        1 gagcgcgagg aacaaggcga caagctgcgc gtgcgcgccg aactgttcgc cgaccattac
       61 agccaggcgc ggctgttctg gaagtcaatg acggagagcg agcaggctca tatcgcgtcg
      121 tcattcacgt tcgagctttc gaaggttgtg ctcgaacatg tgccgtcgcg catgatcgcc
      181 aacttgcgca acgtcgatga agagcttgcc gagcgcattg ccgcgggcat cggcatcgac
      241 ttacccagaa aggccgacgc ccagacgaaa gtactcgacc ttgacccgtc accggcgctg
      301 tcaattcaga aaaatatgaa gaagacactg caaggacgaa aagtcggtat cctctacgcc
      361 gacggatcag acggagcgga gatcaaggcc gtgcagaaag cgatcgagaa ggagggcgcc
      421 acggcctttc tggtcgcgcc gaaggtcggc ggcgccaagc tcaagggcgg caccgtcctc
      481 agggccgacg gtcagctggc cggctcgccg tcgcagttgt tcgacgccgt cgctatcgtc
      541 ttgtccgacc agggctgcga gacgcttatg aatgaggccg gcgctctgca gttcgtcatg
      601 gatgcattcg gacacctgaa agcgatcggt gcgagcaagg ctgccaagcc tctgctcgac
      661 aaagccggcg tcgcacccga tgacggtgtg actggtctcg gcaaggattt catcgaagct
      721 gcgcggatga gattctggga tcgcgagcct tctctgcgca agctcgcctg aaccataagg
      781 cgcactacaa ggcgggctat tattttgggc cgccgacgct aaagttgaat gatgctccgg
      841 cgcgctgtgc atggaagtgg ttggcgcgtc gcggggggag cagtcgccct gaagacgctg
      901 cgtggatttt gcgcatcgtg tccacggtac catatgctca gaaaagggaa caaaatcgcc
      961 ggacggagct tggaatgtcc tgaggaacac gaaagaacgc aaggatttgc aatggccaac
     1021 ggaatgcctt ccatgaccgc gcttctgggc atgcttgctc tggccggcta ccaaaatcgc
     1081 gacaagattt cagagatgct gcgaggcgct acggcgtcgg gaagcgctcc cgggactgca
     1141 tcccaatcgt cgcccctcgg tgggttgctt ggaaatctgg ggggcatgct gggcggcgcc
     1201 ggcgcgggcg gtcttctcag tggcggactt ggcgagttgc tcacgaagtt tcaggcaaac
     1261 ggccatgggg acaccgctcg ctcgtggatc gacaacggcc caaatcagga aatctcgccg
     1321 cccgatctca aacaggccat cggccccgat gtgttggcgg ctttagagca aaagacagga
     1381 ctttcgcaag aggagctgtt ggcgcgtctt tcccgagaat tgccaaacgc cgtggacaag
     1441 tacacgcccg acggccgact tccccactaa cagaaattta ctcacaaacg gaggagacac
     1501 gagatgggca ttctttggac gatcatcatt ggtttcgtcg caggtgtaat cgcgaagttc
     1561 atcatgccgg gcgacaatga gccgtccggc ttcattttga ccgctgtgct cggcatcatc
     1621 ggtgcgttcg ttgcgactta tctcgggcaa gcccttggct ggtatggacc gggacaaggc
     1681 gcgggtctga tcggcgccgt cgtcggcgct gtgatcgttc tccttgttta cggaatggtc
     1741 gcgggccgaa gcagagcgat ttaacactct tcaatcaaag gatttttcac atgggtatct
     1801 tcggaaaaat tatgggcgcg atcttcggct cgcacgcgga agcggccacg ccctctggcg
     1861 gcacaagcag cactgcaact ggtggagcac ccccgacgtc ttcgacgagt ggcactgcga
     1921 aggccactcc catgggcgcg ggcggcgctt cgccgggtcc ggggaccgtg gacgtggcgg
     1981 cgatcctcga taaggccgtc gcaggaaaag gcgaaaagct ggcttggaaa acgtcgatcg
     2041 tcgacctcat gaaggcgctc gatatcgact ccagcctaac ggctcgcaag gacctcgcga
     2101 aagaactggg atataccggc gacacgtcgg actcggcgac gatgaacatc tggctccaca
     2161 aaaaaatgat ggaagaactg gctgccaatg gcggcaagtt gccagcagac atcaagaagt
     2221 cctgaaggtt ggcggtgggc tcgtgtcttc agggacgcga gcctcaaggt agttaactcg
     2281 agcgccggac catgccgttc gcgggaccct cggggcctcg acaatcgaga cgcagaaact
     2341 taccgcccgt gagaacaacg atggtcgagt ggcaagggcg ttttaccttt tcacctccaa
     2401 agctaaacca agccttgtgc gagaaggctc ctcgcggtgt caacaatggt ttgccgcgtc
     2461 ggtcgcgtcg tccagccgag tacgcgccta gctttatctg atgaaaccag tctcttcttc
     2521 cccaaatcga aaagccgacc gcgca
//
LOCUS       DATMGX010000527         3727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_427883, whole genome shotgun sequence.
ACCESSION   DATMGX010000527 DATMGX010000000
VERSION     DATMGX010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427883"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VNR65_08535"
     CDS             complement(<1..352)
                     /locus_tag="VNR65_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ18758.1"
                     /translation="MRIAPAITLSPEQRTLLESQARSRSLPVRVAERARIVLLAASGQ
                     QDKEIAAVMAITPKKVSRWRKRFLALGVSGLEKDAPRPGRKPTIGTRLTKRVVTMTTR
                     QQPTNATHWSTRTMA"
     gene            complement(421..1080)
                     /locus_tag="VNR65_08540"
     CDS             complement(421..1080)
                     /locus_tag="VNR65_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWJ18759.1"
                     /translation="MAELNQEYLRRMEEVLALYEKPLSEKQPVVCIDEKPVVLHQDVR
                     PPLPMRPGRIARRDCEYQRCGTANAFCGVEPKAGRHFTRATPDRSSPRFADYLLAIAE
                     YYPAADTIHLVMDNLSSHTRKAVVERYGEKAGGWLWNRFTVHYTPKHGSWLNQAEIEI
                     SLFSRRLGQRRIPSLCDLRREAWGRRMNRDHITIGWSFTRKQARLKFGYKRNQIMRSE
                     N"
     gene            complement(1022..1543)
                     /locus_tag="VNR65_08545"
     CDS             complement(1022..1543)
                     /locus_tag="VNR65_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ18760.1"
                     /translation="MQTYHRVHVQLGKRDRQQIVGMLNKGRESARVLRRALILRQLNQ
                     GQTAAQVAGNVGVAPKTVRAIARRYAEEGLDSALYEKPRPGKQRRLEAGQSQRIIAMV
                     CSEPPAGQARWSVRLIAQEAVRRKLVPKVGRECIRILLKSHDLKPWREKKLVRGGTES
                     GIPAAHGRGARPV"
     gene            complement(1598..1885)
                     /locus_tag="VNR65_08550"
     CDS             complement(1598..1885)
                     /locus_tag="VNR65_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18761.1"
                     /translation="MFAVLAFNPVLWNVSLARVTREGLYVSLSLAVVALVVTIAFPNP
                     DELSHGRCRIVLPGFSLGLVSAAFWLTREEGIWLLPALGVVIAIALGLRPH"
     gene            2462..>3727
                     /locus_tag="VNR65_08555"
     CDS             2462..>3727
                     /locus_tag="VNR65_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWJ18762.1"
                     /translation="MLPSLIFTASLAMGAVIAGFSFWKGHAVGLDISQVAPFPFVLNV
                     DRLSAFFLLLICAVGAPVALFSVSYVESHYRGARRDLLWALLPLFVLSMIVVVTASTA
                     FAFLFGWELMTLFSSGLILIDGDSQQRRHNTLIYLLMMHAGTAAVVASFFLFLPHAAR
                     LDFASMRGVGGQMSLGLRSAIFLLAFIGFGTKAGIVPLHLWLPRAHPMAPSPVSALMS
                     GVMLKTAVYGFVRFGFDFLGGGPMWWGYLVLAAGAVSGVLGVLYAIAEDDLKRLLAYH
                     SIENIGIIYLGLGAALVFLAHSAPIWAAVALTAALLHSLNHALFKSLLFLGAGAISDA
                     THTVDIEELGGLLRRMPVTGTAFLVACCSIIGLPLFNGFISEWLTFRSFLAGANLVNT
                     SAAVILPLMIGVLALIGGLASACFAKVYGV"
BASE COUNT          650 a         1117 c         1063 g          897 t
ORIGIN      
        1 ccgccatggt gcgcgtgctc cagtgagtgg cgttcgtcgg ttgctggcgc gtggtcatgg
       61 tcaccacgcg tttcgtcagg cgggtaccga tcgtgggttt gcggccgggc cggggcgcgt
      121 ctttctccaa ccccgacacg cccagcgcga ggaaacgctt gcgccagcga gaaacctttt
      181 tcggcgtaat ggccatgacg gccgcaattt ccttgtcctg ttggccggag gcggccaaca
      241 acacaatgcg ggcgcgctcc gccacccgca ccgggagaga tcggctccga gcttgggatt
      301 ccagcaacgt ccgctgctcc ggactcaacg taatggcagg ggctatccgc atccgcgatc
      361 gtctcctcca tcataagacg cacgcggatc tataaaggtt gttaattgtg acgcactaca
      421 ctagttctct gaccgcataa tctggtttct tttgtaaccg aacttcaagc gtgcctgttt
      481 gcgggtaaaa ctccagccga tggtgatgtg atcacggttc attctccggc cccaggcctc
      541 ccgccgcaga tcgcacagcg acgggattct tcgctgcccc agacgccggg agaacaggct
      601 gatttcgatc tccgcttgat tgagccagct tccatgtttc ggcgtgtagt ggacggtgaa
      661 ccggttccac agccagcctc ccgccttctc gccgtaccgc tccaccaccg ccttgcgggt
      721 gtgcgaactc agattatcca tgaccagatg gatggtgtcg gccgccggat agtattccgc
      781 aatcgccagc agatagtcgg caaaccgggg cgaggagcga tccggagtcg cccgcgtaaa
      841 gtgccgtccc gccttcggct ccaccccgca aaacgcgttg gctgtgccgc atcgctggta
      901 ttcacaatcg cgccgggcaa tccgtcccgg acgcatggga agcggaggcc gcacatcctg
      961 gtgcaggacc accggcttct cgtcgataca gaccacgggt tgcttttccg acagcggctt
     1021 ttcatacagg gcgagcacct cttccatgcg ccgcaggtat tcctgattca gttccgccac
     1081 gcaccagctt tttttcccgc cacggcttca gatcgtggct cttgaggagg atgcggatgc
     1141 actcccgccc caccttgggc accagctttc gccgcaccgc ctcctgggca atgagccgaa
     1201 cgctccagcg tgcctgcccc gcgggtggct cgctacagac catggcgata atccgctggc
     1261 tctggcccgc ctccagccgc cgctgcttgc ccggacgcgg cttctcatat agcgccgaat
     1321 ccagcccctc ctccgcgtaa cgccgggcaa tcgcccgcac ggttttcggg gccacaccca
     1381 cattgcccgc cacctgcgcc gcggtctgtc cctggttgag ttgtcgcagg atcaaagccc
     1441 gccgcagtac ccgcgccgat tcccgtccct tgttcaacat accgacaatc tgctgccggt
     1501 ccctcttccc aagctggaca tgcacccgat ggtaggtttg catgtctaca ctatcagtcc
     1561 acttgcctca ctccccaagt gcctactaag gtacatctca atgcggtcgg agacctagtg
     1621 cgattgcgat caccacgccg agagcgggca ggagccaaat accctcctcc cgggtcagcc
     1681 aaaaggccgc actcacaaga cctagactga agcctgggag tacgatacga caacggccat
     1741 ggctgagttc atcagggttc gggaaagcga ttgtcaccac aagagcgacg acagccagtg
     1801 agaggctcac atacaggcct tcccgggtca cccgagcgag cgagacattc caaagaaccg
     1861 ggttaaaagc gaggaccgca aacacgggtg gagcacgcct tcaggcgtgc attgtgggcg
     1921 gcaatttctg atcggcgctt tagcgcctga ggtacatggg tttcattgac ctcagcggct
     1981 gaagccggtt tcccgagtga tcgccaagtg cagcgctgga gcgctgctcc accctataca
     2041 ctaaaagtga aactgctcgt cgccgtcgaa gatcaggaat ttaggtggcc gttcatattc
     2101 ccaggcttcg cgctgttgcc cagcgattac agaagctagc cgatttctgc gggacatttc
     2161 ttcatgggaa cgatggagaa gccaaagccg tttgcctgaa gaagcgctct caagagattc
     2221 agagctcaca acccgagtta tgaagctcat cggcaagggt agacattaat aggcggttca
     2281 ttgttcaatg gacgttctca ggagcgaatc gagtgtgacc cgttgacgct catcgctcat
     2341 gaggcgtagg gcgggttttc gggacatcta aatgatccat gctgcgttca gttcgtttct
     2401 ggtgggattg ctggttgttc cgccaatagt tttggtttct cgccggtgga ggctggtccg
     2461 cttgctgccc tcgctcattt tcacggcctc actggccatg ggcgcagtca tcgccggctt
     2521 cagcttttgg aagggccacg cggttggatt agacatttcc caagtcgcgc cttttccgtt
     2581 tgtgctcaac gttgaccggc tgagcgcgtt ttttcttttg ctgatttgtg ccgttggcgc
     2641 cccggttgct ctgttttcag tttcatatgt cgagtctcac taccggggtg cgcggcgtga
     2701 tttgttgtgg gcgctgttgc ctctgttcgt cttatcgatg atcgttgttg ttaccgcttc
     2761 caccgccttc gcctttttgt tcggctggga gctgatgacc ttgttctcct ctggcttgat
     2821 cctgattgac ggtgactccc agcagcgcag gcacaatacc ctgatttatc tgctgatgat
     2881 gcatgccggc accgctgccg tggtggccag tttttttcta tttttgccgc atgcggcgag
     2941 gctcgatttc gcctctatgc ggggcgtcgg cggacaaatg tcgctgggcc ttcgaagcgc
     3001 aatttttctg ttggcattca tcggtttcgg gacgaaagcg ggcattgtcc ctttgcatct
     3061 gtggcttcca agagcgcatc cgatggcccc tagtccggtt tccgccttga tgtccggggt
     3121 gatgttgaag acggctgtgt atggcttcgt tcgcttcggg ttcgattttc tcggcggcgg
     3181 tcccatgtgg tggggctacc tggtcttagc ggctggcgcc gtctccggtg ttctcggcgt
     3241 attgtatgcg atagcggaag acgacctcaa gcgcctgctc gcctatcata gtattgagaa
     3301 tatcgggatc atttatctgg gtctaggggc ggcccttgta ttcttggcac acagcgcacc
     3361 tatctgggcc gcagtggcgc tcacggcagc tctcctgcat tccctcaatc atgcgctatt
     3421 caagagtctg ctgtttctag gcgcgggcgc catttccgac gccactcaca ccgtcgatat
     3481 tgaagagctt ggcggtctgc tgcgtcgtat gccagtgacg gggacagcat ttcttgtcgc
     3541 ctgttgctcc atcattggat tgcctctgtt caatggcttt atcagcgagt ggctgacatt
     3601 ccggagcttt ttggctgggg cgaacctggt gaatacatca gccgcggtta tcctcccctt
     3661 gatgattggg gttttagccc taatcggagg actcgcttcg gcgtgtttcg cgaaggtcta
     3721 tggcgta
//
LOCUS       DATMGX010000528         3864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_414550, whole genome shotgun sequence.
ACCESSION   DATMGX010000528 DATMGX010000000
VERSION     DATMGX010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414550"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2366
                     /locus_tag="VNR65_08560"
     CDS             <1..2366
                     /locus_tag="VNR65_08560"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase subunit H"
                     /protein_id="HWJ18763.1"
                     /translation="PSLYNVLLRLKEEGYTVEVPASMDDMRERILGGNASRYGTSANV
                     HARVPVNDYIRSERWLPQIEKQWGPAPGKAQSDGATVFVLGEQFGNVFVGVQPAFGYE
                     GDPMRLLFERGFSPTHAFMAFYRYLRDEFGAHALLHFGTHGALEFMPGKQTGLSEDCW
                     PDRLIRDLPNFYLYAANNPSEGTLAKRRGAATIISYLTPPVTQAGLYRGLLDLKGSVQ
                     RWREFAPDVAQEEREALATLIQAQASAVDLADTEPAWPIEEAEDRILALTNKIHELEE
                     TLIPHGLHVVGRPASDDERTDLLTFAGEALDGETPAQETIKAVADGVTIEDALRKAGQ
                     TRTPENLEKLRKLSKMYGYLGKDAELPAIVTALDARYIRPVAGGDIIRNPEILPTGRN
                     IHGFDPFRIPSVFAMKEGEKQAARLLNRHMEEGNALPESVAFVLWGTDNLKTEGEPIA
                     QALAMMGARPRFDGYGRLCGATLVPLEDLGRPRIDVVMTMSGIFRDLLPLQMKLLAEA
                     AYDAATADEPLSENFVRKHALAYQAAHNCDMETAALRVFSNGDGAYGANVNLLVDSGA
                     WQDEDELAETYSRRKSFAYGRQGTPMAQPALLKTMLADVDLAYQCLDSVDLGVTTIDQ
                     YFDTLGGITRSVRRAKGGQDLPVYISDQTRGEGKVRTLNEQVSLETRTRALNPKWYEG
                     MLEHGYEGVRQIEATVTNTMGWSATTGQVDPWVYQRLTQTYVLDEAMRKRLAALNPTA
                     TARMVNRLNEAHERNYWKPDEETREALRKASEELEDQIEGVGMEEAA"
     gene            2363..3301
                     /gene="bchL"
                     /locus_tag="VNR65_08565"
     CDS             2363..3301
                     /gene="bchL"
                     /locus_tag="VNR65_08565"
                     /EC_number="1.3.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:protochlorophyllide reductase
                     (ATP-dependent) iron-sulfur ATP-binding protein"
                     /protein_id="HWJ18764.1"
                     /translation="MTMYERIPSQPIPKFRAKGTGAENEGDGEGSVQVHLDPKVNLGT
                     AKVFAVYGKGGIGKSTTSSNLSVAFSKLGKRVLQIGCDPKHDSTFTLTKCMIPTVIDI
                     LESVNFHSEELRTEDFVFEGYNGVKCVEAGGPPAGTGCGGYVVGQTVKLLKEHHLLED
                     TDVVIFDVLGDVVCGGFAAPLQHAERALIVTANDFDSIFAMNRIAAAILAKSKNYRVR
                     LGGVIANRSRNTDEIDRFNGAIGLNRVAHFQDLDAIRMSRLKKQTLFEMEPTPEIEAV
                     QQEYLRLASGLWAGAEPPVNCKPMKDRDIFDFLGYN"
     gene            3301..>3864
                     /gene="bchM"
                     /locus_tag="VNR65_08570"
     CDS             3301..>3864
                     /gene="bchM"
                     /locus_tag="VNR65_08570"
                     /EC_number="2.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046406 - magnesium protoporphyrin IX
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium protoporphyrin IX methyltransferase"
                     /protein_id="HWJ18765.1"
                     /translation="MPTPTYLERRNDLETYFDRTAADNWAKLTTDAPVSGIRATVRAG
                     RDRMRATLMSWLPDDLHGARLLDAGCGTGLLSAEAARRGAHVVAVDLSPTLIGVAKER
                     MPADVAENIEFHSGDMLDERHGSFDFCVAMDSLIHYPAKDLVNVLAGLADRTASKVVF
                     TFAPKTLALSVMHTAGKFFPRSDRSPAI"
BASE COUNT          787 a         1178 c         1175 g          724 t
ORIGIN      
        1 tcccatcgct ttacaacgtg ctcctgaggc tgaaggagga gggctacact gttgaggtac
       61 ccgcctcgat ggacgacatg cgcgagcgca tcctgggcgg aaacgcaagc cgctacggca
      121 cgtcggcgaa tgttcatgcc cgcgtcccgg tcaatgacta catccgcagc gagcgctggc
      181 tgccgcagat cgagaagcaa tggggcccgg cgcctggtaa ggcgcaaagc gatggcgcca
      241 ccgtgttcgt tctcggcgag caattcggca acgtctttgt cggcgtgcag cctgctttcg
      301 gctatgaagg cgacccgatg cggctcctct tcgaaagagg cttttcgccg acacacgcct
      361 tcatggcctt ctaccgctat ctgcgcgacg agttcggcgc gcatgcgctt ttgcacttcg
      421 gcacccacgg cgcgctggaa ttcatgccgg gcaaacagac gggtctctcc gaggattgct
      481 ggccggaccg cctcatccgc gatctgccga acttctacct ttatgctgcg aacaacccgt
      541 ccgaaggcac gctcgccaag cggcgcggcg cagccaccat catcagctat ctgacgccgc
      601 ccgtgacaca agctggactt tatcgcgggc tcctggatct taagggctcg gtgcagcgct
      661 ggcgcgaatt tgcccccgat gttgcccagg aggagcgtga agcgctcgcg acactcattc
      721 aggcgcaggc ttccgcggtg gatctcgcag acactgagcc ggcttggcca atcgaggaag
      781 cggaagaccg catcctcgcc ctcacgaaca aaatccatga gcttgaggaa acgctgattc
      841 cgcacggcct gcacgtggtc ggcaggcccg cgtccgatga cgagcgcacc gatcttctga
      901 ccttcgcagg cgaagctctt gatggcgaaa ctccggcgca agagacgatc aaagccgtag
      961 ccgacggcgt gaccatcgag gacgcgctaa gaaaggccgg gcaaacgcgt acgccggaaa
     1021 acctcgaaaa gctgcgcaag ctgagcaaga tgtacggcta tctgggcaag gacgccgaac
     1081 tgcccgccat cgtgacagcg cttgatgctc gctatattcg tcccgtggcg ggtggcgaca
     1141 tcatccgaaa ccccgaaatc ctgcccaccg gacgcaacat ccacggtttc gatcccttcc
     1201 gtattccgag cgtgttcgcg atgaaggaag gcgaaaagca ggctgcgcgc ctcctcaata
     1261 gacacatgga agaaggcaac gcgctgcccg agtcggtcgc attcgtgctg tgggggacgg
     1321 ataacctaaa gaccgaaggc gagccgattg cgcaagcgct ggcgatgatg ggcgctcgtc
     1381 cgcggttcga cggctacggc cgcctgtgcg gcgcaaccct cgtcccgttg gaagatctcg
     1441 gccggccaag gatcgacgtg gtcatgacca tgtcgggcat cttccgcgat cttctgccct
     1501 tgcagatgaa gcttctcgct gaggccgctt atgatgcggc gacggcggat gagccgttat
     1561 ccgagaactt tgttcggaaa cacgcgcttg cctatcaggc cgcacacaac tgcgacatgg
     1621 aaaccgccgc gctccgcgtc ttctcgaacg gcgatggcgc ttacggtgcg aacgtcaacc
     1681 tcctcgtcga tagcggcgct tggcaggacg aggatgagct tgcggaaacc tacagccgcc
     1741 gcaagtcttt cgcctacggc cggcaaggca cccctatggc gcagccggcg cttctgaaga
     1801 ccatgctggc cgatgtcgat ctcgcctatc aatgtcttga ctcggttgat ctcggcgtca
     1861 ccaccatcga ccagtatttc gatactcttg gcggtatcac ccggtccgtg cgccgcgcca
     1921 agggcggcca ggacctgccg gtctacatca gcgaccagac gcgcggcgaa ggcaaggtgc
     1981 ggacgctgaa cgaacaggtt tcgctcgaga cgcgcacgcg cgcgctcaac ccgaaatggt
     2041 atgaaggcat gctggagcac ggctatgaag gcgtgcgcca gatcgaggcg accgtcacca
     2101 acacgatggg atggtcggcg accacaggac aagtcgatcc ctgggtctat caacgcctca
     2161 cgcaaaccta tgtgctcgat gaagccatgc gcaaacggct cgccgcgctt aaccccacgg
     2221 cgacggctcg catggtgaac cggctgaacg aagcccacga gcgcaactat tggaagcccg
     2281 atgaggagac gcgcgaagcg cttcgcaagg cttccgagga actagaagac cagattgaag
     2341 gggttggcat ggaggaagcc gcatgacaat gtatgagcgt atcccgtcac agccgattcc
     2401 caaattccgg gccaaaggca ccggcgctga aaacgaaggc gacggcgaag gcagcgtgca
     2461 ggttcacctc gatccgaagg tgaatctcgg gactgcgaag gtttttgcgg tctacggcaa
     2521 gggcggcatc ggcaagagca cgacctcgtc caacctgtcg gtcgcttttt cgaagctcgg
     2581 caagcgcgtc ctgcaaatcg gctgcgatcc caagcacgac agcacgttca cgctgacgaa
     2641 gtgcatgatc ccgacggtca ttgacatcct cgaatccgtg aacttccatt ccgaggaatt
     2701 gcgcactgaa gacttcgttt tcgaaggcta taacggcgtc aaatgcgtag aggctggcgg
     2761 accgcctgct ggcacgggct gcggcggcta cgtcgttggc cagacggtta agctcttgaa
     2821 ggagcatcac cttctcgaag acaccgatgt cgtgatcttc gacgtgctgg gcgacgtggt
     2881 gtgcggcgga ttcgcagcgc cgcttcaaca tgcggagcgc gctttgatcg tcaccgcgaa
     2941 cgattttgat tcgatcttcg ccatgaatcg tattgcggcc gcgatcctcg ccaagtcgaa
     3001 gaactaccgg gttcgtctcg gcggcgtgat cgccaatcgc agccgaaaca ctgacgaaat
     3061 cgaccgcttc aacggtgcca tcgggctcaa tcgcgtcgcg cacttccagg atcttgacgc
     3121 catccgcatg agccgcctga agaagcagac attgttcgag atggagccga ctcccgagat
     3181 tgaggccgtg cagcaggagt atctgcgcct cgcatccggt ctttgggctg gtgcggagcc
     3241 gccggtcaac tgcaagccga tgaaagatcg cgatattttc gatttcctgg gatataattg
     3301 atgccaaccc ccacctatct cgagcgccgc aacgacctcg aaacctattt cgacaggacg
     3361 gctgccgaca attgggcgaa gttgacgacc gatgcgccgg tgagcgggat tcgcgccact
     3421 gtgcgcgccg gccgcgaccg gatgcgcgca acgcttatga gctggcttcc ggacgatttg
     3481 cacggggcgc ggcttcttga tgcaggctgc ggcactgggc ttctttcggc cgaggcggcg
     3541 aggcgcggtg cgcatgtcgt cgctgtcgat ctttcaccaa cgttgatcgg ggttgccaag
     3601 gagcgcatgc cggcagatgt tgccgaaaac atcgagtttc actcgggcga catgctcgat
     3661 gagcgtcatg gcagcttcga cttttgcgtt gccatggatt cgctcattca ttaccctgcg
     3721 aaagaccttg tgaacgtgct cgcggggctt gcggacagga cggcatccaa ggtggtgttc
     3781 acgttcgcgc ccaaaacttt ggcgctcagc gtgatgcaca ctgctggaaa gttttttccg
     3841 cgttcggacc gctcgcctgc gatc
//
LOCUS       DATMGX010000529         2709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_238220, whole genome shotgun sequence.
ACCESSION   DATMGX010000529 DATMGX010000000
VERSION     DATMGX010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238220"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(943..2475)
                     /locus_tag="VNR65_08575"
     CDS             complement(943..2475)
                     /locus_tag="VNR65_08575"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HWJ18766.1"
                     /translation="MIEIPEQPEQVKPRPQGPPKLRTVNRLQTILATIYVEELIAADH
                     KARAIWQLVESLDLSRFCEPLRTTVGCVGRSAWDPRLLVSVWVYAYSEGISSAREIER
                     LMQWEPGLQWLSGTVSVNHHTLSDFRVEHKPALDELFAEMLAMLESAEVLSLEQVMHD
                     GTKIRALAGADSFRREKTLRERLREARALVAQMGDPRADEPAKDRKQAARERANRERV
                     ERLEAAQKELSALQAEKKTEEEKAAVRVSLTEPEARLMKHGDNAIAPSYNAQISTEGK
                     SKIIVGAHLSQCSSDAQSLQPALEVVEENLGEKPKQVVVDGGFTNRTNIVKCDAQGID
                     LVGSMANSQERSEAAMKSQGIDPAFAPHHFHILEAGQQLQCPAGNILPYVRQNQKRGD
                     LYHLFQARSADCLACRYQPQCCPKTAVKGRTVSIRVEEQAEMAAFRQKMELPENRAIY
                     RRRGEVAEFPNAWIKDKLGLRKFNVRGMAKAGSELLWACLTYNIQQWVRLVWLRRPVA
                     VS"
BASE COUNT          500 a          922 c          718 g          569 t
ORIGIN      
        1 aatctcccca tcgggcagtg gcccccaagg cccgtggtgt cttgcgccac catctcccca
       61 gtccagccgc ccaggccgcc agctttgagc gaggtgcgct tcggcgctcg cgagaagtga
      121 cccaccggtg ctctccaaaa gtgacccagg cattctgacc tgaccgcggc gagcctgcgc
      181 gtgatgacaa cgatagatca tcacgatgcc agaggcccgg aagagaccat gcaggatctg
      241 ccgccggtgg ttccggccgg gtccgcgagc aggagaccgg cagcgggcct gcgacgcgcc
      301 agaatgtcag gccgcccgcc ggcagaagac gcaggcgagg tggcgcaacc ggaacccagg
      361 ctatgccgtc gcctggagaa ttgaccggcg cgcgacgcaa gcgcaacaac cgccggagcc
      421 gctgcgattg ccggcgccat tgaaccaact tccctgggat gtcgcgaaag accacttcgg
      481 agtgcaaggg gctgatttca ttggggttat gagttcacta atcatccgtg tcgcgaaaga
      541 ccacttcaag gcgtatctca ttgatcctac gggactttcc ggcacacttc ccccgccgcc
      601 agaaaagacc agtcccggct tcaagcatac tgaatcccga gcagacgacg atgcaactgg
      661 agtttcacca actggaccgg cgatgggagc atctgcgagt ccgcgagccg caccggcaga
      721 aggcgtgaaa tattgtccgg cattggctga gggggataaa atcagaccca cgattccgca
      781 acttaattat tatcgctgac cgccttggcc cgacttccgt ctccgcccag ctcggctact
      841 taggctccag agtagcgttt ccctaggcta aaatgctcat tcctaggccc ttagagccgt
      901 ttcccagctt tgccgctcct ttctccaggt ggctggctga acttaggaaa ctgccaccgg
      961 ccggcgcagc cataccagcc gcacccactg ctggatgttg taggtcagac aagcccacaa
     1021 caactcgctt cccgccttgg ccatgccccg cacgttgaac tttctcaaac ccagtttgtc
     1081 cttaatccag gcgttgggaa actccgccac ctcgccccgc cgccggtaga tcgctcggtt
     1141 ctccggcaac tccatcttct ggcgaaacgc cgccatctcg gcctgttctt ccacccggat
     1201 cgataccgtc cgccccttga ccgccgtctt cggacaacac tgcggttgat agcggcacgc
     1261 caggcaatct gcgctgcgcg cctgaaacag atggtacaga tctccccgct tctggttctg
     1321 tcgcacgtac ggcaggatgt ttcccgccgg acactgcaac tgctggcccg cttccagaat
     1381 gtggaaatga tgcggggcaa aggccggatc gatcccctgc gatttcatgg cggcctcact
     1441 gcgctcttgc gaattcgcca tcgaccccac tagatcgatc ccttgggcat cgcacttcac
     1501 aatgttggtg cggttggtga agcctccgtc caccaccact tgcttgggtt tctcacccag
     1561 attctcctcc actacttcga gtgccggttg caggctttgg gcatcgctgc tgcactggct
     1621 caggtgcgct cccacgatga tcttgctctt tccctcggtg ctgatctgcg cgttgtagct
     1681 cggtgcgatg gcattgtccc catgtttcat cagccgcgct tccggttccg tcaagctcac
     1741 ccgcacggcg gctttttctt cttccgtctt cttctccgcc tgtagcgcac tcagctcttt
     1801 ttgcgccgct tccagccgtt ccacccgctc ccggttggcg cgctcacgcg ccgcttgctt
     1861 gcgatctttc gccggctcgt ccgcccgcgg atcccccatc tgcgccacca gggcccgggc
     1921 ctctcgcaac cgttcccgta gggttttttc tcgtcgaaag gaatccgctc ctgccagcgc
     1981 ccggatcttc gttccgtcgt gcatcacctg ctccaggctc aacacctcgg cgctttccaa
     2041 catcgccagc atctccgcaa acaactcatc cagagccggc ttatgctcca cccggaaatc
     2101 cgacaacgtg tgatgattga ccgataccgt tccgctcagc cattgcaggc ccggctccca
     2161 ctgcatcagc cgctcgatct ctctggccga actgatcccc tcgctatagg catagaccca
     2221 cacgctcacc aacaaacgcg ggtcccacgc cgaccgaccc acgcagccta ccgtagtgcg
     2281 caatggctcg cagaaacggc tcaagtccag actttccacc aactgccaga tggcccgcgc
     2341 cttatgatcg gcggcgatca attcttctac atagatcgtg gccagtatgg tttgcaggcg
     2401 gttcaccgtt ctcagtttcg gcggtccttg cggccgcggc tttacctgct ctggctgctc
     2461 ggggatttcg atcaataccg cctgagaggg tgcgtccata aatccattat gaagcatgag
     2521 cttcataatg aaaagtactt cactattaaa agcaagattt tttcacagct tctgacggtc
     2581 gcggctccga tcagagccgc gcgcgccagc aagcggtttt ccgatacgtt gggggacttc
     2641 tgaaacggcg tactaagcgg cgatccggga gcgcgcgaac gtcgacgggc cggcgcccga
     2701 ggtcgtgcg
//
LOCUS       DATMGX010000530         2535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_355221, whole genome shotgun sequence.
ACCESSION   DATMGX010000530 DATMGX010000000
VERSION     DATMGX010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355221"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..994)
                     /locus_tag="VNR65_08580"
     CDS             complement(293..994)
                     /locus_tag="VNR65_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWJ18767.1"
                     /translation="MSAEQLERLSSIARRRTVPANQYIFRDGDEAISFAAIVSGVVKL
                     IKTTAEGERHIIGLVYAPEFLGHTFADQHRFSAAAATDVDMCTFPRASFNRLLDEYPE
                     LERWLFEFTVRELDISRDWTLMLGRKSSYERVASLLLIIARRARNAGCQPASDNSAHF
                     ELPLTRSELADYLGLTLETVSRKISGLKRKGVIELRTTREIVVPDIELLAEIANMDPI
                     GEDIMGRRQVAVHHS"
     gene            complement(1177..1950)
                     /locus_tag="VNR65_08585"
     CDS             complement(1177..1950)
                     /locus_tag="VNR65_08585"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004517.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWJ18768.1"
                     /translation="MAFETVLFEKKGRVGLITLNRPQARNALNDQLMAELNQVLDQCE
                     ADAEIGAIVLTGSEKAFAAGADIKEMKDKSFADVVAHDFFAPWERLSTCRKPVIAAVS
                     GYALGGGCEIAMMCDFIIASETARFGQPEIMLGVIPAVGGTQRLTRAVGKSKAMDLIL
                     TGRHMDAAEAERCGLVARVVPASEVVNEALKAAEKIASYSLPSVMMAKEAVNRAFEST
                     LAEGILFERRTFRSLFATEDQKEGMAAFAGKRSAQFKHR"
     gene            complement(1962..>2535)
                     /gene="mutM"
                     /locus_tag="VNR65_08590"
     CDS             complement(1962..>2535)
                     /gene="mutM"
                     /locus_tag="VNR65_08590"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWJ18769.1"
                     /translation="HDHVVFGMSNGDVIRYNDVRRFGSMSLFEAKDMAEHPLFKALGV
                     EPLSDDLSPSYLAERASGKAQPLKAFLLDQRIIAGLGNIYVCEALFRAGLPPDAEAGL
                     LAKGKRGRAASERLCRSIKRVLEDALLAGGSSIRDYRHADGGGGSFQEQFDVYGRGGE
                     PCHNTCGAFIHRKMQQGRSTFYCPSCQAKL"
BASE COUNT          531 a          750 c          756 g          498 t
ORIGIN      
        1 catgccggtc tggaaaaaaa tcttcgcgca tgaatgcggg gacgcctatg cgcgccaagc
       61 cgaaatcgaa ctcgaaaaat ttctgagggt gatccaggaa aaatagacgg tgccgccggg
      121 gccacagaga atattttggc gatgcaatag ctgcgcggat gagcccatta tattggcttc
      181 cggtgttttt ccccggcgac aaggcttagt attcgctttg gccgcagctt ggatttgcga
      241 aaacacaaac aacggacgaa aaagttaggt gtgtgtgttg gtccggcgcc tgttagctat
      301 gatgaaccgc aacttggcgc cgtcccataa tatcttcccc gatggggtcc atattcgcaa
      361 tttcggccag aagctcgata tcaggcacca caatctcgcg ggtcgtgcga agttcgatga
      421 cgcccttccg tttgagaccg ctgatcttgc ggctcacggt ttcgagggtc aggccgagat
      481 agtcggcaag ctcggagcgc gtgagaggca actcaaagtg agcggaattg tcggaagcag
      541 gctggcagcc cgcattgcgc gcgcgcctcg cgatgatcag caacaggctg gcgacgcgct
      601 cataggacga tttccgccca agcatgagcg tccaatcgcg gctgatgtcg agctcgcgaa
      661 ccgtaaattc gaaaagccac cgctcgagct ccggatactc gtccagcagg cggttgaagg
      721 acgcgcgcgg aaacgtgcac atgtcgacat cggtggcggc agcggccgaa aagcggtgct
      781 gatcggcgaa agtatggccc aggaactctg gcgcgtagac aagcccgata atgtggcgct
      841 cgccctcggc agtcgtcttg atgagcttga caacgcccga aacgatcgcg gcgaaagaga
      901 tcgcctcatc gccatctctg aaaatatact ggttggccgg taccgtgcgc cggcgcgcga
      961 tcgagctcag cctctcaagc tgctccgctg acaaggcagc gcaaattccg cggtgccgga
     1021 taaagcaggc ttcgcatctg ccgggcgcgc tgtgagcacg gtcagctgta tggatggaca
     1081 ttgtcttcaa gcgcaagccc cgaaggcata ttgtgtggtt tgttccaacg taaagtctga
     1141 acgcctgcct gtccaatacc tttgcgatag atcaaatcaa cggtgcttaa attgggcgga
     1201 gcgcttgccc gcgaatgctg ccatgccctc cttctgatcc tctgtggcaa agagagaccg
     1261 gaaggtgcgt cgctcgaaga ggattccctc ggccaaggtg ctctcaaagg cccgattcac
     1321 ggcttccttc gccatcatga cggatggaag cgaatagcta gcgatcttct ccgcggcctt
     1381 cagggcctcg ttcacaacct cggaagcggg cacgacacga gcgacgagcc cgcaccgttc
     1441 ggcctctgct gcgtccatgt gccgcccggt gagaattagg tccatggcct tggatttgcc
     1501 cactgcgcgc gtgagccgct gggttccccc cacggcggga atcaccccga gcatgatttc
     1561 gggctgcccg aacctcgccg tttcgctcgc gatgatgaaa tcgcacatca tggcgatttc
     1621 gcagcctccg ccaagggcgt agcctgagac cgcggcgata accggcttgc ggcaggtgct
     1681 gaggcgctcc cagggtgcga agaagtcgtg cgcgacaacg tcggcgaagg acttgtcctt
     1741 catctccttg atatcggcgc cggcggcaaa agccttctcc gagcctgtca ggacaatggc
     1801 gccgatctct gcatccgcct cgcactgatc cagaacctgg ttcaactcgg ccatgagctg
     1861 atcgttcagc gcattacgcg cctgggggcg gttgagcgtg atgagcccca ctctgccctt
     1921 cttttcgaaa agcacagtct caaaggccat ggcgattcct ttcacagttt ggcttggcag
     1981 ctcgggcaat agaaggtgga gcggccttgc tgcattttgc ggtgaataaa ggcgccacaa
     2041 gtattgtgac aaggctcgcc gccgcgtcca tagacatcaa attgttcctg gaagctgccg
     2101 cctccaccgt cagcgtggcg gtaatcgcgg atcgaggagc cgcctgcgag gagcgcatct
     2161 tcgagcacac gtttgatgct cctgcaaagc cgctccgatg ccgctcggcc tcgcttgccc
     2221 ttcgcgagca gacccgcctc ggcgtctggc ggcaagccgg cccggaacaa cgcctcgcag
     2281 acataaatat tgccaagccc cgcgatgatc cgctggtcga ggagaaaggc cttgagcggc
     2341 tgcgccttgc cggaagcacg ttcggcaaga taggaaggcg agaggtcatc cgacagcggc
     2401 tcgacgccaa gcgccttgaa gagcggatgc tccgccatgt ccttcgcttc gaaaagcgac
     2461 atggaaccga agcgccggac atcgttgtac cggatcacgt cgccattgct catgccgaac
     2521 accacatggt cgtgg
//
LOCUS       DATMGX010000531         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_351512, whole genome shotgun sequence.
ACCESSION   DATMGX010000531 DATMGX010000000
VERSION     DATMGX010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351512"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VNR65_08595"
     CDS             <1..292
                     /locus_tag="VNR65_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ18770.1"
                     /translation="AIAQSWRRNWEHVIPFFAFPEGVRRIIYTTNAIEALNAKLRRAV
                     RTRGHFPTDEAAIKLLYLVLRQVADEWKMPPREWCEAKTQFAIMFDERFVTA"
     gene            395..1615
                     /locus_tag="VNR65_08600"
     CDS             395..1615
                     /locus_tag="VNR65_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HWJ18771.1"
                     /translation="MARRKQPAISDELLDQLLAGADPKTAFAKDGLLDELKKAFAERA
                     LNAEIDHHLNNGEEDGRPNSRNGYGRKSVLTETAKIELEVPRDRRSTFDPQLIAKYQR
                     RFPGFDEKIVSMYARGMSVREIQGHLRDLYGVDVSADLVSAVTDAVLEEIAEWQNRPL
                     EPLYALVFFDAIRVKVRDEGMVRNKAVYVALGVRPDGTKEILGLWIEQTEGAKFWLRV
                     MNELKNRGVEDVLIAVVDGLKGFPEAITAVFPQTQVQTCIVHLIRNSLDFVSYKDRKA
                     VAAALKQIYRAKDAAAGEEELNAFAEGPWGRKYPAIAQAWRRHWSEVIPFYAFPDEVR
                     RIIYTTNAIESLNAKLRRAVRARGHFPTDESALKLLFLVLNLAQKEWKMPPREWAMAK
                     AQFAIIFEGRFKLA"
     gene            1768..1995
                     /locus_tag="VNR65_08605"
     CDS             1768..1995
                     /locus_tag="VNR65_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18772.1"
                     /translation="MKHKLAGRIVRVEIHIQDANGDALRLQPINDFAQMPDRARQAVN
                     FRDNQGIAFARKLDCRFKLFPRRDRADVLAE"
BASE COUNT          510 a          651 c          649 g          460 t
ORIGIN      
        1 ggcgatcgcg cagagctgga ggcgcaattg ggagcatgtc atcccctttt tcgcgttccc
       61 cgagggtgtt cggcggatca tctacacgac caacgccatc gaggccttga acgccaaact
      121 gcgccgcgcc gtgcgaacga ggggacattt tccaacggac gaggcggcga tcaaattgct
      181 ctatctcgtc ttgcgccagg tcgcggacga atggaaaatg ccgccgcgcg aatggtgcga
      241 agcgaaaact caattcgcca tcatgttcga cgaaaggttc gtcacggcgt gacggtcaac
      301 ccggcctcgc acacaaaatt cctgacagac cctactgagg tctgtcggaa atttcgtgtg
      361 agggccataa cgatgggaaa gaaggagaca tcgtatggcg agacggaagc aaccggctat
      421 ttccgacgag ctgcttgatc agcttttggc gggagccgac ccgaagacag cctttgcgaa
      481 ggacgggctg ctcgacgagt tgaagaaggc gtttgccgaa cgagccttga acgccgagat
      541 tgatcatcat ctcaacaacg gcgaggaaga cggccgcccg aatagccgga atggctatgg
      601 ccgaaaatcg gttctcaccg agaccgccaa gatcgagctt gaagttccgc gcgatcgacg
      661 gtcgaccttc gatccgcaac tgatcgccaa atatcagcgg cgatttcctg gtttcgacga
      721 gaagatcgtc tcgatgtatg cgcgcggcat gagcgttcga gaaatccagg gccatttgcg
      781 cgatctctat ggggtcgacg tctccgctga tctcgtcagt gcggtcacgg acgctgtcct
      841 cgaggagatc gccgaatggc agaaccggcc gctggagccg ctctatgcgc tggtgttctt
      901 cgacgccatc cgggtcaagg tgcgcgacga aggcatggtg cgcaacaaag ccgtctacgt
      961 cgcccttgga gttcggcccg acggaaccaa ggaaattctc ggcctgtgga tcgagcagac
     1021 cgagggcgcg aaattctggc tgcgcgtcat gaacgagctg aagaaccgcg gcgtcgaaga
     1081 cgtcctgatc gccgtcgtcg atggcctgaa gggctttccg gaggcgatca ccgcggtgtt
     1141 tccacaaacc caggtccaga cctgcatcgt tcacctgatc cgcaattcgc tggacttcgt
     1201 ttcctacaag gaccgcaagg ctgtcgcagc ggcgttgaag cagatctacc gggccaagga
     1261 cgccgccgcc ggagaggaag aactgaacgc cttcgccgaa ggcccatggg gccgaaaata
     1321 cccggcgatc gcccaggcct ggcgacgcca ttggagcgag gtcatcccgt tctacgcctt
     1381 tcccgacgaa gtgcgtcgga tcatctatac gacaaacgca atcgagtcat tgaacgccaa
     1441 attgcggaga gcggttcgcg cccgcggcca tttccccacc gacgagagcg cgctgaagct
     1501 tctttttctc gtgttgaacc tggcccaaaa agaatggaaa atgccgccac gcgagtgggc
     1561 catggcgaaa gcgcaattcg cgatcatatt cgagggcagg ttcaagttgg cgtaacgaaa
     1621 ccggccttta cacggaaatc ctgacagtct cgcgcgggct gtggggatgc cgccgcgata
     1681 tttgccttcc gcctttgcct tggcgatgcc ttccaactgc cgcgacagca tgatgcttct
     1741 ttcaaactcg gcaatcgatc cgatgatttg aagcataagc ttgccggtcg catcgttcgt
     1801 gtcgagattc atattcaaga tgcgaacggc gacgcccttc gcctgcaacc gatcaacgat
     1861 ttcgcacaaa tgccggaccg agcgcgccaa gcggtcaatt ttcgtgacaa ccaaggtatc
     1921 gccttcgcgc gcaaactcga ttgccgcttc aagctgtttc cgaggcgcga ccgagctgac
     1981 gttctcgctg aatagtttct cggctcctgc cgccttcaaa tcccggatct gcgcttcaag
     2041 accggcgacc tgttcaatgg tcgaagttct gccgtaggca ataatcttaa aatatccctt
     2101 ttccatcaaa tatatgccaa cagatacaaa gatacgatgg catacaagta tgccgatatc
     2161 aaaaactatt tacatggcat agtgtttaag gggaagctcg cctatgccat gcgggcctgg
     2221 tctgttggca tgctttgcca tttgcccgct gacttcaagc aagcgggcgg
//
LOCUS       DATMGX010000532         2636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_159266, whole genome shotgun sequence.
ACCESSION   DATMGX010000532 DATMGX010000000
VERSION     DATMGX010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159266"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /gene="obgE"
                     /locus_tag="VNR65_08610"
     CDS             complement(<1..862)
                     /gene="obgE"
                     /locus_tag="VNR65_08610"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWJ18773.1"
                     /translation="MRFLDQAKVYIRSGAGGNGCLSFRREKFIEFGGPNGGDGGRGGD
                     VLVECVSNLNTLIDFRYQQHFKASNGKHGMGSNMTGAAGDCAVLKVPPGTQVFAEDNE
                     TLLADLVEPGQRALIAAGGNGGFGNTHFKSSTNRAPRRVNPGQPGIEMTIWLRLKLIA
                     DAGIIGLPNAGKSTFLAAVSAAKPKIADYPFTTLHPNLGVVKVGDTDFVLADIPGLIE
                     GAHEGAGIGDRFLGHIERCRVLLHLVDITEADVARAYRTVRGELKAYGADLNRKREIV
                     ALSKCDAVTPQ"
     gene            complement(960..1175)
                     /locus_tag="VNR65_08615"
     CDS             complement(960..1175)
                     /locus_tag="VNR65_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWJ18774.1"
                     /translation="MRQTGTVKFFNQAKGFGFITPDEGGKDVFVHISAVERSGVGMLD
                     EGMRVTYETQPDPKGKGPKAVNLERSE"
     gene            complement(1490..1927)
                     /locus_tag="VNR65_08620"
     CDS             complement(1490..1927)
                     /locus_tag="VNR65_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18775.1"
                     /translation="MVRYVIIAALAVASVLFAYRDHQASRTAEQQSQLVIETRERAEK
                     ELQSLRDRLAAEERARQVAEKDAKAVSAKLAEEQTARDAAEQTAKAASERLSQVQNAL
                     ANAEQASRDAREALDKAQKDRDQALETLASERSHAAAQSKPAG"
     gene            complement(2073..>2636)
                     /locus_tag="VNR65_08625"
     CDS             complement(2073..>2636)
                     /locus_tag="VNR65_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HWJ18776.1"
                     /translation="LPIVSAKLIEATDIIIDAKSGVSGAGRSLKQNILFSEAGEGLSP
                     YSVASHRHAPEIEQEISAAAGETVLVNFTPHLVPMSRGELVTAYVRLKDNHKAEDLAA
                     ALAQQYADAPFVHIAPKGTLPSTQFVRGSNYCMLGVYADRIPGRAIVISAIDNLVKGS
                     AGQAIQNFNLMFDVPENTGLEQLPLFP"
BASE COUNT          514 a          755 c          757 g          610 t
ORIGIN      
        1 cctgtggcgt gacggcatcg catttggaca acgccacgat ctcgcgcttg cgattgagat
       61 cggcaccata cgccttcagt tcacctcgca cggtgcggta tgctcgggcg acgtccgctt
      121 cggttatgtc gacaaggtga agcagcacgc ggcaacgctc gatgtgacca agaaaacggt
      181 cgccgatccc ggcgccttca tgggcaccct cgatcagacc tgggatgtcc gccaggacga
      241 aatctgtatc cccaactttg accacgccga ggttcggatg aagggtggtg aatgggtaat
      301 ccgcgatttt cggcttcgcc gcgctgactg ccgcgaggaa ggtggatttc ccggcattcg
      361 gcagaccgat gatccccgca tccgcgatga gcttaaggcg gagccagatc gtcatttcga
      421 tgccgggttg gccgggattc acgcgtcgcg gcgcgcggtt cgtagaagac ttgaagtgtg
      481 tgttcccaaa gccaccattt ccgcctgcgg cgattaatgc tcgctgccct ggctcgacga
      541 gatcggcgag aagggtctcg ttgtcctcgg caaagacctg ggttcccggc ggaaccttca
      601 gcacggcaca atcgccagct gcgccggtca tgttggaacc catcccgtgc ttgccgttgc
      661 tggccttgaa atgctgctga tatctgaaat caatcagcgt gttcaggttg gaaacgcact
      721 caaccaaaac atccccgccc cggccgccgt cgccgccgtt cggtcctccg aattcgataa
      781 atttttcacg ccggaaggaa aggcagccgt tcccgccggc ccccgagcga atgtagactt
      841 ttgcttgatc taagaagcgc atcaaccgca attaagctgt cttgcgctcc aacgcaagca
      901 atgccgtgtg ggtaattttg gaaagcccag aagagcggat tgcccccgag tttgccagct
      961 tattcggagc gctcgagatt gacagccttt ggacctttac ccttgggatc gggctgggtc
     1021 tcataggtta cgcgcatgcc ctcgtcgagc attcccactc ccgaacgctc gaccgcggaa
     1081 atgtgaacga acacatcttt cccgccctcg tccggtgtga taaaaccaaa acccttcgcc
     1141 tgattaaaaa atttgaccgt tccggtctgg cgcatgggat agcactcctc ttccccgacg
     1201 aaaaaaacat ggccgagaga ccatgttttg gcttagcagg cagaacagtt atcgggggaa
     1261 gacatgcgca catttttgcc gcaagctgcg gtttattctc aacgcgtagt tccacggggt
     1321 ccgatagttt gcccgattgg ggcggattat gctcgatctt agctatttcg taaagagctt
     1381 ggcgataagt tttcaaacac ttcacttgct cggcttggta gtgatgcaag aacgcatcag
     1441 tttcctctgg aaaatggcgg ggctctcgat tgccgttcgc ttttatggcc taaccggcag
     1501 gctttgactg cgccgcggcg tggctgcgtt cgctcgccaa agtctccaaa gcttgatctc
     1561 tgtctttctg cgccttgtcc agggcttcgc gagcgtcccg agaagcttgc tcagcatttg
     1621 ccagcgcgtt ctgaacctgc gacagacgct cgctcgctgc cttggcggtt tgctcggctg
     1681 catcgcgtgc cgtttgctcc tcagcgagct ttgcggagac agctttcgcg tccttctccg
     1741 ccacttgccg cgcgcgttct tctgccgcaa gcctatcgcg caaggattgc agttcctttt
     1801 cggccctctc gcgtgtctcg atcacaagct gggactgctg ctctgctgtc ctgctcgctt
     1861 ggtgatcgcg ataggcaaac agaacggatg ctacagccag ggcggcgata atgacgtagc
     1921 ggaccatggt gtcctcgggc ggagcggcgt aaaagggaaa cctggaaacg agtcctcgct
     1981 ccttatcata acggcttgtg gccgattatg gcatttaggc aggcgaaata cttttccaac
     2041 ctgtgtggtt ttgatgcata tcgcgctgcc ccttacggga aaagaggcag ttgctcgagg
     2101 ccagtgtttt ccgggacatc aaacatcaga ttgaaatttt gtatggcttg gcccgctgag
     2161 cctttcacaa gattgtcgat tgccgaaatg acgatggcgc gcccaggaat gcggtcggcg
     2221 taaacaccca gcatgcagta gttcgatccg cgcacgaatt gcgttgaggg gagcgttcct
     2281 ttcggcgcta tatgcacgaa tggggcatct gcatattgct gggcgagcgc ggccgcgaga
     2341 tcctcggcct tatgattgtc tttgaggcgg acataggcgg tcacaagctc gccgcggctc
     2401 attggaacga gatgcggtgt gaagttcacg aggacggttt ctcccgctgc cgccgagatt
     2461 tcttgctcga tctccggcgc atgccggtga ctggcgacac tgtacgggga aagcccttct
     2521 ccggcctccg agaaaaggat gttctgcttg agcgagcgac cggcgccgga cacgcccgac
     2581 tttgcatcaa taatgatgtc cgtggcttcg atgagcttcg cgctcacgat gggcag
//
LOCUS       DATMGX010000533         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_289043, whole genome shotgun sequence.
ACCESSION   DATMGX010000533 DATMGX010000000
VERSION     DATMGX010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289043"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VNR65_08630"
     CDS             <1..835
                     /locus_tag="VNR65_08630"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWJ18777.1"
                     /translation="DPEPPPLRAIAEHIPLDIIYEDDDLAVINKPAGMMVHAGAGATD
                     SERNRGTLVNALLHRFAKLSDIGGELRPGIVHRLDKETSGLIIVAKNDVTHRKLGQQF
                     SGRQVKKRYVALVHGWPAKDRGTINTEIGRDAVRRIRMTTRGRGGRSAITHYEVRERI
                     ASQFGKFALVDVRIETGRTHQIRVHLSSIGHPVVGDSLYRAPRELRRPTALRPASTKA
                     TSRGASQPEARQTPSVLTLGRNFLHAAAIEFTHPRTGDPLSFSAPLPPELTQLLEQIR
                     G"
     gene            1051..1320
                     /locus_tag="VNR65_08635"
     CDS             1051..1320
                     /locus_tag="VNR65_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18778.1"
                     /translation="MVDPGFAGGFVVGAGRSEPDTAAASYRAIGDEVPSPAIRSAATL
                     RDQPIQYSGAVTGPGSANPRGPKFFIAGNGSTDRLFATVHFRRIG"
     gene            1388..2218
                     /locus_tag="VNR65_08640"
     CDS             1388..2218
                     /locus_tag="VNR65_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWJ18779.1"
                     /translation="MRKRVDEVNVVFTVTDKRGHFVKDLTQNDFRVFDDAKPAESVRS
                     FSRETNLPLRVGLLIDASNSVRDRFKFEQEAAIEFLNQIIRSKYDKAFVIGFDTTPEV
                     TQDITDDTEALSRGVRMLRPGGGTAMYDAIYYACRDKLMQADKSQVATRRAIILLSDG
                     EDNQSRVSRDEAVEMAQRAEVIIYAISTNTSGLKLRGDKILEHFADETGGKAFFPFKI
                     QDVADAFSQIQDELRSQYAVSYKPADFAPNGKYRKIEILADNKKYKVRARKGYYAPRQ
                     "
     gene            2336..>2729
                     /gene="mutL"
                     /locus_tag="VNR65_08645"
     CDS             2336..>2729
                     /gene="mutL"
                     /locus_tag="VNR65_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWJ18780.1"
                     /translation="MGRIHVLSENVANKIAAGEVVERPASVVKELLENALDAGARRIR
                     VSVEAGGKKLIQVTDDGCGMVRDDAMLAFERHATSKIRNADDLLNIATLGFRGEALPS
                     MASVSRLRLETHAPEDAAGTVVEIAGGKL"
BASE COUNT          583 a          865 c          784 g          497 t
ORIGIN      
        1 cgatcccgaa cctccgccgt tgcgcgccat cgcagagcac attcctctcg acatcatcta
       61 cgaagacgac gacttggctg tcatcaacaa gccagcgggg atgatggtgc acgccggagc
      121 aggagccacc gacagcgaac gcaatcgcgg gacgctggtc aacgccctgc tgcaccgctt
      181 cgccaagctg tcggatatag gcggcgagtt gcgcccggga attgtgcacc gtctcgacaa
      241 ggagaccagc gggctgatca ttgtcgccaa gaacgacgtg acccatcgca agctgggcca
      301 gcaattttcg gggcggcagg tgaagaagcg ttacgtggcg ctagtgcatg gctggccggc
      361 gaaagaccgc ggcaccatca atactgagat cggtcgcgat gcggtgcggc gcattcgcat
      421 gacgacccgt ggccgcggag gccgctcagc catcacccat tatgaagttc gcgagcgcat
      481 agcctcgcaa tttggtaagt tcgcgctggt ggacgttcgc attgagacgg gccgcaccca
      541 tcagatccgc gtgcacctgt cgtcgatcgg ccatcccgta gtgggcgact cgctgtaccg
      601 ggcgccgaga gaactgcgcc gacctactgc tctgcgaccg gcctcaacca aagcgacctc
      661 tcggggcgca agccagccgg aagccaggca gacgcccagc gtactcaccc tcggtcgcaa
      721 cttcctgcat gcggcagcca tcgagttcac acatccccgc accggtgatc ccctttcctt
      781 cagcgccccg ctgcctccgg aattgacaca gcttctggaa cagattcgag ggtgaggagc
      841 agggtggcgc gtactgaagt gtggggccga tctttgacac cagcctgaaa ggctggctct
      901 acccaagaat tcttttttca ccacggagga cacggagatc acggggtttc tattgttatt
      961 tcttctccgt gccctccgtg ttctccgtgg tgaaaacgga acgtccgtgg cgacctcgcc
     1021 agcggctata atggaatttc gaatgaaaac ttggttgatc ctggttttgc tggcggcttt
     1081 gtcgtggggg ctggccgctc agaacccgac acagcagcag cttcctaccg cgccatcggc
     1141 gacgaagttc cctccccagc aatccgctcc gccgccaccc tccgggacca gccaatccag
     1201 tactccggcg cagtcaccgg ccccggcagc gccaaccccc gcggacccaa gttcttcatc
     1261 gcaggtaacg gctccaccga ccgcctcttc gcaaccgtcc acttccggcg catcggataa
     1321 atcgggtacg gcctctaccg ctcgctcgac cggccaaagc gacaatcccg acgataccct
     1381 gaccgtcatc cggaaacgcg tggacgaggt caacgtagtc tttaccgtca ccgacaagcg
     1441 tggccatttc gtaaaagacc tgacgcaaaa cgatttcaga gtttttgacg acgccaagcc
     1501 cgcggagagc gtccgctctt ttagtaggga aaccaacctg ccgctgcgtg tgggattgct
     1561 catcgacgcc agcaactcgg tgcgcgatcg cttcaagttc gaacaggaag cggccatcga
     1621 gttcctgaat cagatcatcc ggtctaaata cgacaaggcg tttgtcatcg gcttcgatac
     1681 cacgcccgag gtgacgcagg acatcaccga cgataccgag gcgctctccc gtggcgtgcg
     1741 catgctgcgc ccgggcggcg gtaccgcgat gtacgacgcc atctactatg cctgccgcga
     1801 taagctgatg caggccgaca aaagccaagt ggccacgcgg cgcgccatca tcctgctcag
     1861 cgatggcgaa gacaatcaga gccgcgtctc ccgcgacgaa gccgtggaaa tggcgcaacg
     1921 cgccgaggtc atcatctatg ccatttcaac caataccagc gggctgaagt tacgaggcga
     1981 caaaatcctc gaacacttcg ccgacgaaac cggtggcaag gccttcttcc ctttcaagat
     2041 tcaagacgtg gccgacgcct tctcgcagat tcaggacgaa ctgcgcagcc agtacgcggt
     2101 ttcctacaaa ccggccgatt tcgctcccaa tggcaagtac cgcaaaatcg agattctcgc
     2161 cgacaacaaa aagtacaagg tgcgcgcccg caagggatat tacgcgcccc ggcagtaacg
     2221 caggggctag tggttagggg gccaggggtt agttcaagaa gtcttgaact cgaaacttga
     2281 aactcaaaac tgagatactt tctctgtgcc tctgtggtga attactcttg aagtcatggg
     2341 ccgcatccac gttctctccg aaaacgtagc caacaagatc gccgccggcg aagtcgtgga
     2401 gcgacccgct tcggtggtca aggagctgtt ggaaaacgcg ctggatgccg gtgcgcgccg
     2461 catccgggtc agcgtcgaag cgggcggcaa gaagctgatc caagtaacgg acgacggctg
     2521 cggcatggta cgcgacgatg ccatgctggc tttcgagcgc cacgccacct ccaagatccg
     2581 caatgccgac gacttgctga acattgcaac cctgggattt cgcggagagg cgctgccttc
     2641 gatggcttcc gtcagccgcc tgcgtttgga gacccatgcg cccgaagacg ctgccggaac
     2701 ggtggtcgag atcgccggcg gcaagctgt
//
LOCUS       DATMGX010000534         3659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_254831, whole genome shotgun sequence.
ACCESSION   DATMGX010000534 DATMGX010000000
VERSION     DATMGX010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254831"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..447
                     /locus_tag="VNR65_08650"
     CDS             211..447
                     /locus_tag="VNR65_08650"
                     /inference="COORDINATES: protein motif:HMM:NF040101.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6429 family protein"
                     /protein_id="HWJ18781.1"
                     /translation="MDIDRDHIDDAVLALLFLGRHDGMRTWKSFDWAAMERLHTKGLI
                     SDPVGKAKSVVFTDEGLRQSEALFRKLFTVGHEA"
     gene            complement(578..925)
                     /locus_tag="VNR65_08655"
     CDS             complement(578..925)
                     /locus_tag="VNR65_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18782.1"
                     /translation="MKGQPISVSKAKKAIADYKKAIGRPAGMAELSIFYCEEAFGFLE
                     SCSMDDEKYFAALIHMYGRSLEIVSSLPPVERSTYLERLGRLRSRGRNVGWVVEEEFN
                     SLWYDAVDEQQGE"
     gene            980..1114
                     /locus_tag="VNR65_08660"
     CDS             980..1114
                     /locus_tag="VNR65_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18783.1"
                     /translation="MTQAQAGMQECLVVFARSVEVLHKPSKARAIEVQERGLNVAPRW
                     "
     gene            complement(1641..2252)
                     /locus_tag="VNR65_08665"
     CDS             complement(1641..2252)
                     /locus_tag="VNR65_08665"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HWJ18784.1"
                     /translation="MRLRDDRARSDVCRVAGFIGSCSRGAEQFTIRWLLERLNRHAFG
                     IVILLLALVGMVPGICVLAAILLLIPASELIAGRVVPTFPRRLADRPFPTRYLTGAVR
                     RAIPVLLHVEKAIHPRWCIPTEITNRVVGAIVILMAILLVAPIPMIQVFPASVIATIA
                     LAHIEDDGLLLAIGVAGAVAMSAAAFITIWEIILGADWISGLF"
     gene            complement(2239..3522)
                     /locus_tag="VNR65_08670"
     CDS             complement(2239..3522)
                     /locus_tag="VNR65_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWJ18785.1"
                     /translation="MPFFLKKRSIVSLVVVFCLLVAAMAASAILFDRQTRDATAVQHT
                     LQVQGHLTRVLSLLQDAETGQRGYLLTGNPAYLQPFTSATSDLDKTIEGLGLEVADNV
                     TQVQALGTLRLVINDKLDELLATIALKAANKSEEVLRRTLGEHIAVETVLAGGLWRTH
                     ADPGQLENAIVNLAVNSRDAMPDGGKLTIETANASLDETYAAQHVDVPAGQYVLLAVS
                     DTGQGMPADVVAKAFEPFFTTKGLGKGTGLGLSQVLGFVRQSGGHVKIYSEPHHGTTI
                     KIYLPRFMGAADELLPVVPVEEAPAGQQHEVVLVVEDEERMLQVSCDAFRELGYTVLQ
                     AGGPIAALRVIDVHPEITLLFTDVVMPEMNGRQLAREAVLRLPKLKILYTTGFSRNAV
                     IHNGVLDQDVNFISKPFTIEQLASKARAVLDGAAS"
BASE COUNT          800 a         1086 c         1030 g          743 t
ORIGIN      
        1 tcgcgcggca tgtccgttcg accctcagga gcagacatcg tcaggctaca agctcaggtc
       61 cggttcgtgc cgttcgcaat attttgcacc gcaaaataca gcacattatt cgatcgtccg
      121 gtcgacgatg gcaccgcagc ccttatccgc gtccaatcgc cgaggtatcg tcaggttcgg
      181 cgatcaacat cacctcggag gtcatcgatc atggacatag accgagacca catcgacgat
      241 gctgttcttg ccttattatt tctcgggcgt cacgacggga tgcgcacctg gaaatccttt
      301 gattgggccg cgatggagcg gctccataca aagggattga tctcggaccc agtcggcaaa
      361 gctaaatccg tcgtattcac tgacgaaggc ctgcgtcagt ccgaagcatt atttcgaaag
      421 ctgttcacag tcggacacga agcatgaggc gcgttatccc tgaacccagc ctgactgttg
      481 cgcaagctcc gccaccggcg acccagccgc tacttcaccg atcaatgcaa tgaactcccg
      541 ccactccatc gatcacctcg tctgacagaa agagcgctca ttcaccctgc tgctcgtcca
      601 ccgcatcata ccaaaggcta ttgaattctt cctcgaccac ccagccgaca ttcctcccgc
      661 gtgacctaag cctgccgaga cgctcaaggt aggtgctgcg ttcgacagga ggcaggctag
      721 atacgatttc cagagaccgt ccatacatgt ggatcaaagc agcaaagtat ttttcgtcgt
      781 ccatgctgca agattccaaa aagccgaatg cttcctcgca ataaaatatc gatagctccg
      841 ccatcccagc cgggcgtccg atggcctttt tgtagtccgc aatcgccttt ttggctttgg
      901 aaaccgatat gggttggcct ttcattaggt ccggcaatat ccatctggaa atactcgctt
      961 tgaagggccg gagctgatca tgacccaagc ccaagcgggc atgcaagaat gcctggttgt
     1021 ctttgctcgc agtgtagagg tcttgcacaa gccctcgaag gcccgcgcga tcgaagtcca
     1081 ggagcgcggt cttaacgtcg ctccaagatg gtgagatatt cttgcttcgg accacggcca
     1141 tgaatccttc aaaagtgctt ttatgcgcgc gcctctcagc tggctcaaga taccggactt
     1201 acgcataggg agcagatcgg tcaggtcaac gatgcttcac cagtgggaca tgctcccctc
     1261 ctctgtaacc gaggagtatg accatgctgg atcaagtgaa agctaccgac gcctcataaa
     1321 cggttgcctt ggaacaaggg caagctgacc ggggcaaagc cgccgctacg acccaagcac
     1381 gtctggtcga tccggacgac gctccagatc gaaagccgcg ctcgcgccat gttcaatctg
     1441 gccatcgaca gcaagcttcg tggtagcaac ctggcgcacg aacggccgct ttgcgccaaa
     1501 agcggtccct cgaccctctg agcgcttccg gaagaagcgg atcgtcaatt ccggacgcgc
     1561 cagccttcct gcaacttttc tgcgacacag cgcttcccgc gaacggcaaa cgcgggtctg
     1621 cagaaggcct tgttacacct tcaaaacagt ccgctgatcc aatcggcgcc caaaattatc
     1681 tcccagatcg tgatgaaggc tgccgctgac atagcgaccg caccggcgac tcctattgcg
     1741 agcagcaggc cgtcgtcttc gatgtgggca agcgcgatgg tggcaatgac cgatgcaggg
     1801 aaaacctgga tcatcgggat tggagcaacc agcaggatag ccatcaaaat cacgatagcg
     1861 ccgacgaccc gattggtgat ttctgtcgga atgcaccaac gtggatggat cgctttctcg
     1921 acatgcagca gcaccggaat tgctcgtcgg acagcgccgg ttaggtatcg tgtcggaaac
     1981 ggacggtccg cgagccgtct gggaaatgtc ggcacgacac gtccggcgat caattccgaa
     2041 gcgggaatga gaagcagaat cgccgccagc acgcagatgc caggcaccat cccgacaagc
     2101 gccagaagca aaattacgat gccgaacgca tgcctgttca gccgctccag cagccaacgg
     2161 atcgtaaact gctccgctcc gcgggagcat gatccgataa atcccgcaac acgacagacg
     2221 tcggaacgtg cgcgatcgtc acgaagccgc accgtcaaga actgctcgcg ccttggaggc
     2281 gagctgctcg atggtgaatg gcttggatat gaaattcacg tcctgatcca gtacgccatt
     2341 gtgaatgacc gcgttgcgac tgaacccggt cgtgtacagg atcttcagct tcggcaatcg
     2401 taaaacggct tcgcgcgcga gttgacgacc gttcatttcc ggcatcacca cgtcagtgaa
     2461 cagcagggtt atttccgggt ggacgtcgat gacgcgaagt gccgcgatag gtccaccggc
     2521 ttgcagcacc gtgtatccga gctcccggaa ggcatcgcaa gagacctgca gcattcgctc
     2581 ctcgtcttcg accaccagta caacttcatg ctgctgaccg gcaggggctt cttccacggg
     2641 aacaaccggc aacaattcgt cggcggcgcc cataaatcgt ggcaaataga tcttgatcgt
     2701 cgtgccgtgg tgcggctcgg aatagatttt cacgtggccg cctgactgcc tgacgaaacc
     2761 gagcacctgg ctaaggccaa ggccggtgcc tttgccgagc cctttcgtcg tgaagaatgg
     2821 ctcgaatgcc tttgcaacga cgtcagcggg cattccctga ccggtgtcgc tgacggccag
     2881 caagacgtat tgaccggccg ggacgtcaac gtgctgagcg gcgtaggtct cgtcgagact
     2941 ggcgtttgct gtctcgatgg tcagcttgcc gccgtcaggc attgcgtcgc ggctgttgac
     3001 ggcgagattg acgatcgcat tttcgagctg gccgggatcg gcgtgggttc gccacaggcc
     3061 gccagcgaga acggtttcga cggcaatgtg ttcgccgagc gtccgcctta gcacctcttc
     3121 cgacttgttc gctgctttga gcgcaatcgt tgcaagtaac tcgtccagtt tgtcgttgat
     3181 gaccagcctg agggtcccga gcgcctgaac ctgggtgaca ttgtcggcca cctccaggcc
     3241 gagtccctcg atggtcttgt ccaggtccga cgtcgccgac gtgaagggct ggaggtacgc
     3301 cgggttgccg gtcaagagat atccccgctg accggtttcg gcatcctgga gcagcgacag
     3361 cacgcgcgtc aggtgaccct ggacttgcag tgtgtgctgg acggcagtgg cgtcgcgtgt
     3421 ctgccggtca aagagaatgg cggatgccgc catcgcggcg accagaagac agaacacgac
     3481 cacaagcgag acaatcgaac gcttctttag gaaaaagggc attagtccta tctaaatttt
     3541 ggctgatcga cctgacgatc gtgcggaatc accgctgagg cgggcgggta gccactggct
     3601 gatgcggcga actatagcgt ataggttgcc gcgttggcac caattgcgac gcggcggcc
//
LOCUS       DATMGX010000535         4221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_152372, whole genome shotgun sequence.
ACCESSION   DATMGX010000535 DATMGX010000000
VERSION     DATMGX010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152372"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VNR65_08675"
     CDS             <1..1097
                     /locus_tag="VNR65_08675"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HWJ18786.1"
                     /translation="TGSLGKRTNYEVRFHNTVSGLLAGSAVLFNGIRVGEVTGLKLVP
                     NEPKEIDVTIAVDATTPMRADTQASLDFQGLTGVAVVTLTGGSADAPVLKASSGQLPT
                     LTASEAAGQTMSEAARQAFARIDKVLAENSGDLRTMVSNLTSFSQALGKNSGKVDAIL
                     GGLERMTGGGKTAGLVYALSALPAFTEGAKPLEKQLAIADPSALLAYDSDRVLAQMDG
                     DELAPIGNAKWADSLTKLVQSRIVQSFENTGSLNEVSRPIEGGAAEFQLATEIRKFQI
                     VNAPERKAEAQISAKVIAADGHIVAARVFDGSAPVTSAEEADAAKGLNEAFGKILSNL
                     IPWTADVIAGGGEKPQPAPAQPRKRGQAGG"
     gene            1375..1905
                     /locus_tag="VNR65_08680"
     CDS             1375..1905
                     /locus_tag="VNR65_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18787.1"
                     /translation="MSGNMGMRLSCLVKELAALKQELAALKRLPGPQGEKGDKGDQGE
                     KGEKGAIGAKGEKGDTGEEGKKGDKGDRGEKGDKGDPGAKGEKGERGAKGDKGEPGPK
                     GDKGDLGSKGDKGDPGPKGDKGDTGPKGDKGSKGDNGEKGDKGDPGKKGDKGDPGEKG
                     EKGDSGAAAAPNKREQ"
     gene            complement(2026..3258)
                     /locus_tag="VNR65_08685"
     CDS             complement(2026..3258)
                     /locus_tag="VNR65_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HWJ18788.1"
                     /translation="MNEKPYDLVAFGATSFVGKLLCRYLAHEFGFDGRLRWAAAGRSR
                     GKLEALRSSLGDGAAALPIEIADAADEAALATICAKTRAVVSTVGPYAFYGEPLVKIC
                     ATTGTDYCDLTGEVPWIKRMIGLYEAKAQSSGARIVHCCGFDSIPSDMGVTFLQQEAR
                     RHFGQPCLHVKMRVKAIRGGASGGTIASLLNVATDVAASPELRRELANPYAFCPPIDV
                     PKVKQASLSRPEYDPDFDSWLAPFVMASINTKVVHRSNALSNFAYGRDFLYDEAMLAG
                     HGVEGGAKAYAIAAGLGAFVAASVIPPARWALERFVLPAPGEGPSPESQKRGFFDLRF
                     FGKTADGSAIRTKVAGDADPGYLSTSKMLGQAAACLAALDKPEKPGGFWTPATCFGDR
                     LIEALKARAGISFQLLDG"
     gene            3418..>4221
                     /gene="hrcA"
                     /locus_tag="VNR65_08690"
     CDS             3418..>4221
                     /gene="hrcA"
                     /locus_tag="VNR65_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWJ18789.1"
                     /translation="MSRRQELRAPALDLKGAGGVRELNERYREIFRSIVETYLATGEP
                     VGSRNLSRHLPMTLSPASVRNVMSDLETLGLIYAPHVSAGRIPTHTGLRLFVDGLLEV
                     GCIEDAERAQFERQLHDYARDKGTVTSEVLAEAGDMLSGLSRCAGVVLADKQVSRIKH
                     IEFVSLDSKRALLVLVGDDGGVENRVLNLPEGLPQQALSEATNYLNAKVRGATLPEAR
                     ERVEAELKASQFELDELTARLVRSGLAHWSGGRDDTKSLIVRGQSRLLDD"
BASE COUNT          969 a         1151 c         1340 g          761 t
ORIGIN      
        1 cgaccgggag cctcggcaaa cgcaccaact atgaggtgag atttcacaac acggtttcag
       61 gcctcttggc cgggtccgcg gtgcttttca acggcatccg cgttggcgag gtgacagggc
      121 tcaaacttgt tccgaatgag ccgaaagaga tcgacgtgac gatcgcggtc gatgccacca
      181 cgccgatgcg agctgacacg caggcatcgc tcgacttcca gggcttgacc ggtgtggccg
      241 tggtcacgct cacaggcggc tcggcggacg cgccggtgct caaggcttcc tcagggcagc
      301 ttcctacgct gaccgcaagc gaggcggctg gtcagacgat gagcgaggcg gcccggcagg
      361 ctttcgcacg catcgacaag gttctcgcgg aaaactccgg cgatctcaga accatggtct
      421 ccaatctgac ttcattctcc caggcccttg gaaagaattc cggcaaggtg gacgccatcc
      481 tcggaggcct tgagcgcatg acaggcggag gaaagacagc cggcctcgtt tatgctctca
      541 gcgcgctgcc agcttttacc gagggggcga agccgcttga gaagcagtta gccatcgcag
      601 acccgagcgc cctgcttgct tacgactccg accgtgttct cgctcagatg gatggagacg
      661 aactcgcgcc gattggaaac gcgaaatggg ctgatagcct gacgaagctc gttcaatcga
      721 ggatcgtgca aagcttcgag aataccggct cactcaacga agtgagccgc ccgattgagg
      781 ggggcgctgc cgaatttcag ctcgcaaccg agattcggaa gtttcagatc gtcaacgcgc
      841 ctgaaaggaa agcggaggct cagatcagcg cgaaggtcat tgcggccgac ggccacatcg
      901 tggcggctcg cgtttttgac gggagcgctc cggtgacgtc ggcggaggaa gcagacgctg
      961 ccaaagggct gaacgaggcc ttcggcaaaa tcttgtccaa tctgatcccc tggacggcgg
     1021 atgtcatcgc gggagggggc gagaaacctc agcctgcgcc agcccaacct cgcaaacgtg
     1081 ggcaggcggg cgggtaaaga gggattccgt cttgcgccca cccaaatacg gggacagcag
     1141 cgcattacaa attgggtagc ctatgacgct tgggcgttat tcacgttttc gtgagtaaac
     1201 tgttttggat gcggatcttt attgtcctcg cactcgtctc cggatcacta gctgagatat
     1261 tgcggatcgg aagggaggag aaaaaatatg cgacttcatt cggttggcgc ttttgtgttc
     1321 ctctgcgtca cgggtctgtg cgcttcagag gcacgatctc aagacgcggc ttgcatgagt
     1381 ggcaacatgg gcatgcgctt aagctgcctc gtaaaggaac tggcggcact caaacaggaa
     1441 cttgcggccc ttaagcggct gcctggcccg cagggtgaaa aaggggacaa aggcgaccag
     1501 ggagaaaaag gcgagaaggg agctatcgga gcgaaggggg aaaagggaga taccggggag
     1561 gagggaaaaa aaggagacaa gggggatcgt ggggagaaag gcgacaaagg tgatccgggc
     1621 gccaaaggag agaagggaga acgaggagca aagggcgata aaggagaacc aggaccaaag
     1681 ggtgataagg gagacctagg atcaaagggt gataagggag acccaggacc aaagggtgat
     1741 aagggggaca caggaccaaa gggtgataag gggagcaaag gagataacgg ggagaaggga
     1801 gataagggag accctgggaa gaagggagat aagggagacc caggtgagaa gggtgaaaag
     1861 ggagactcag gagctgcggc ggcgccaaac aagagagaac aatgattaga ctgagcaaat
     1921 gcgccgaaat gatcttctgc ttttcgtccg gccatggttt tgatttttgc gaaccgcaaa
     1981 ccaaaacctg gaagtcaaaa tgttggtgaa gccacagaat tctgctcagc cgtccaatag
     2041 ctgaaaagag ataccggcgc gcgctttcag tgcctctatg agacgatcgc caaaacatgt
     2101 tgcaggcgtc cagaagccgc ccggcttttc cggcttgtcg agagccgcga ggcaggccgc
     2161 tgcctggccg agcattttcg atgtcgagag atatcccgga tcggcgtcgc ctgcgacttt
     2221 cgtgcggatc gcgctgccgt ccgccgtctt gccgaaaaag cgcaggtcga aaaatccccg
     2281 cttctgcgac tcaggcgatg gtccttcgcc tggagccggg aggacgaagc gctccaaggc
     2341 ccaccgcgca ggcggaatca cgctggccgc cacaaacgca ccaagccccg ccgcgatagc
     2401 gtaagccttc gcgccgccct ccacgccgtg gcctgcaagc atggcttcgt catataagaa
     2461 gtcgcggcca taagcaaaat tcgacagagc attggaccgg tgcaccacct tggtgttgat
     2521 gctggccatg acgaagggcg cgagccacga gtcgaaatcg gggtcgtatt ccggccgcga
     2581 aaggctggct tgcttgacct ttggaacatc aatcggcggg caaaacgcgt aagggttggc
     2641 aagctccctt cgcagttcgg ggcttgccgc aacgtcagtg gcgacattca agaggctggc
     2701 gatggtgccg ccagaggctc cgccccggat cgccttgacg cgcatcttca catgcaaaca
     2761 gggctgcccg aaatggcggc gcgcttcctg ctgcagaaag gtgaccccca tatcagacgg
     2821 aatagagtcg aaaccgcagc aatgaacgat ccgtgcgcct gacgactgcg ctttcgcctc
     2881 ataaagcccg atcatgcgtt tgatccaggg aacctcgccg gtcagatcgc agtaatcggt
     2941 gccggtagtg gcacaaattt tgacgagagg ctcgccataa aacgcgtacg ggccgacggt
     3001 cgagactacg gcgcgggttt tggcgcatat cgtcgcaagc gccgcctcgt ctgccgcatc
     3061 ggcaatttca atcgggagtg cagcagctcc gtcgccaagc gaagagcgca gcgcctcaag
     3121 cttgcctcgt gaacggccag cggcagccca cctcagccgg ccatcaaagc caaattcatg
     3181 ggcaagatag cggcataaga gctttcccac aaaacttgtg gccccaaacg caacgaggtc
     3241 ataaggcttt tcgttcatct attttgcaaa actccctgct caattaggcc actggaccgg
     3301 cattactggt gttatcccgc cttgtacaca tataagttag agaagcgtcc ccggtttgac
     3361 tgcaacgatt ggggagacta gatttagtag gtttgcagca ggcgataagc gaagacgatg
     3421 tcgagaaggc aggaactacg cgctcccgcc cttgacttga agggggcagg cggcgtccgc
     3481 gaattgaatg agcgctaccg ggagatcttc cggagcatcg tagaaactta cctcgcgacg
     3541 ggcgagccgg tcgggtcgcg caatctaagc cgtcatctcc cgatgacctt gtcgccagcc
     3601 tcggtccgga acgtcatgtc ggatcttgag acgctggggc tgatctatgc cccccatgtc
     3661 agcgccgggc gaattcccac gcataccgga ttgcgcctct tcgtagacgg acttctcgaa
     3721 gtgggctgca tcgaggatgc ggaacgggcg cagttcgagc gccagctcca tgattacgcc
     3781 cgcgacaagg gcaccgtcac gtccgaggtt ttggccgaag ccggcgacat gctctccggc
     3841 ctgtcgcggt gcgccggtgt tgtcctggcc gataagcagg tgagccgcat caagcatatc
     3901 gaatttgtga gccttgattc gaaacgcgcg cttctcgtgc ttgtgggaga tgatggcggc
     3961 gttgaaaacc gtgttttgaa cttgccagaa gggctgcctc agcaggcact ctccgaagcc
     4021 accaactatc tgaatgcgaa ggttcgtggc gccacgttgc cggaagcgcg agagcgggtc
     4081 gaggccgagc tgaaagcgag ccagttcgag ctggatgagc ttacggcgcg tcttgtccgg
     4141 tcgggcctgg cgcattggtc cggcggaagg gatgacacca agagcctcat cgttcgcggg
     4201 cagtcgcgcc ttctcgacga t
//
LOCUS       DATMGX010000536         2829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_159570, whole genome shotgun sequence.
ACCESSION   DATMGX010000536 DATMGX010000000
VERSION     DATMGX010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159570"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1135
                     /locus_tag="VNR65_08695"
     CDS             158..1135
                     /locus_tag="VNR65_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HWJ18790.1"
                     /translation="MPALLAPGGTLEMARTVIDEGADIVYVGPLGWSRRPHESEMTDD
                     DIHAAVRYADAHGKQVRVVLNTFPSPFEMDDFIEKVRLYDSWGVSGFIVTDPGAIALI
                     RQQLPHTLIHVSIGSGVTNSLDARFYKSLGADVIILPYRWGADEIEATRETSDIGLEV
                     FLFETVQTGKVCPGKCIMSSYLKFRDWLDIEGKDHFFGSANRGAKECYRVCQTQWDFA
                     SEDSEPVSLKLRRDARLMLEQLPHFIGLGVECFKISGRERPTSMIRDLVRFYRKVIDG
                     ITTGSQTDMSVYVDELAVLRVRWNVEKRKRVGVLMDRAGTYTQEGALQA"
     gene            1132..2067
                     /locus_tag="VNR65_08700"
     CDS             1132..2067
                     /locus_tag="VNR65_08700"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ18791.1"
                     /translation="MTPPLGSLRAAFEATGSPSWVMRVICKQGFDRRHGFHLDLTRSG
                     DQGSRTAQATQGALVAGETDLIDTDWISIARSRQTGVELVPICPYGRIMGGMVTAAGS
                     GINDLSDLPGRRVGVIRAIDKNWIVIRAAFRQRHGSDLNEQAIVKEALSKTTLLEWLK
                     AGEVDAAVLPWHLVPRMTAAGGRFRQLCDVLDLIPELGATSVPTTFFVVQADFANPKQ
                     ELISAFTAAYTEAVNLMRAEETVWREAAAGPDDSPDMLAALRLSWLRRIQYEWGPDDG
                     AHLESLFERLKSIAGEKALGIDHMPRGLFGPAFMQ"
     gene            2079..>2829
                     /locus_tag="VNR65_08705"
     CDS             2079..>2829
                     /locus_tag="VNR65_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HWJ18792.1"
                     /translation="MVALMGPGGTLKMAVRALESGCDSVFVGPKGWSRRPATDELADG
                     EIRELIEWSAPRGKDVRIAINVMPAPDEIDVFLSKVERYAAWGANGVMICDPGCIKLV
                     HERLPQLDIHVSVTAGIFNLRDIQFYRSLGANIVVIPYRWGVAELEEIRDQTGVGLEA
                     FMFQSMRRGRICPGRCYSSSYFKIAHTVDATGKNHFIGSASRGGSCHRICRAKWDLTA
                     DSELMGQPDLKGTPELLLWEMPDYIRLGVNRF"
BASE COUNT          545 a          841 c          912 g          531 t
ORIGIN      
        1 cgcatctatc gcgaagcact gacgaaggct ttcactggcc agccgctggg cgatcagagc
       61 gcagcgctgc aggaactcgc cggacttgcc aagatggggc tctgcaacgg ctactattac
      121 gaaagcgctg gccgccgcta cgtcgggagg gtttgatatg ccagcgctgc ttgcgccggg
      181 cggcacgctg gagatggcca gaacggtcat cgacgaaggg gccgatatcg tttacgtcgg
      241 tccgcttggc tggagccggc ggccccacga atccgagatg accgacgacg acatccacgc
      301 cgctgtccgc tatgccgatg cgcacggcaa gcaggttcgt gtggtgctga acacgtttcc
      361 cagcccgttt gagatggacg atttcatcga gaaggttcgt ctttacgaca gttggggcgt
      421 gtccggcttc atcgtcaccg atccgggtgc aatcgctctc atccgtcagc agctaccgca
      481 tacgctgatc catgtcagca tcggcagcgg tgtcacgaac tcgctcgacg cgcgcttcta
      541 caagagtctc ggcgcagacg tgattatcct gccctatcgc tggggcgcag acgagatcga
      601 agccacccgc gaaacctccg acattgggct ggaggtcttc ctcttcgaaa cggtccagac
      661 cggcaaagtc tgccccggca aatgcatcat gtcgagctat ctcaagttcc gcgactggct
      721 cgacatcgag ggcaaagacc acttcttcgg aagcgcgaac cgaggcgcga aggaatgcta
      781 ccgcgtttgc caaacccagt gggatttcgc gagcgaggac agcgaacccg tcagtctcaa
      841 gctgcggcgc gacgcccggc ttatgctgga gcagctgccg cacttcatcg ggcttggcgt
      901 cgagtgcttc aagatttctg gccgcgagcg gccgacttcg atgatccgcg atctcgtccg
      961 cttctatcgc aaggtgatcg acggcatcac aactggtagc cagaccgaca tgagcgttta
     1021 cgtggatgaa ctcgcggttc tgcgcgtgcg ctggaacgtc gaaaagcgca aacgcgtcgg
     1081 ggtgctgatg gaccgcgctg gcacctacac gcaagaaggc gcactgcaag catgacgccc
     1141 cccttgggat cattgcgggc agccttcgaa gccacaggca gcccttcctg ggtgatgcgg
     1201 gtcatttgta agcagggctt cgaccgccgc cacggcttcc acctcgatct cacgcggagc
     1261 ggggaccagg ggagcaggac ggctcaggca acacaggggg cgctggttgc gggcgagacg
     1321 gatcttatcg acaccgattg gatctcgatc gcccgcagcc gccagaccgg tgtggaactg
     1381 gttccgattt gcccttacgg gcgcatcatg ggcggcatgg tgacggcggc cgggtcgggg
     1441 ataaacgatt tgtcggatct gccgggacgg cgcgtcggcg tcatccgcgc gatcgacaaa
     1501 aactggatcg tgatccgcgc cgcctttcgt cagaggcacg ggtccgatct caacgagcag
     1561 gcgatcgtca aggaagcgct gtcgaaaacg actttactgg aatggctcaa agccggtgag
     1621 gtcgacgccg cggtgctgcc ttggcacctt gtcccgagga tgacggcggc gggcgggcgg
     1681 tttcgccagc tctgcgatgt gctcgacctc attccagagc ttggggcgac ctcggtaccc
     1741 acgacgttct ttgtagttca agctgatttt gccaacccaa agcaagagct gatttcggct
     1801 ttcaccgctg cttacaccga ggcggtaaac cttatgcggg ccgaggaaac ggtctggcgc
     1861 gaagcagcgg cagggccgga tgacagcccg gatatgctgg ctgccctgcg cctgtcctgg
     1921 ctccggcgca tccaatacga atggggaccg gacgacggcg ctcacctcga aagccttttc
     1981 gagcggctca agagcatcgc aggcgagaaa gccctcggga tcgatcatat gccgcgaggc
     2041 ctgttcggcc ctgcgttcat gcaatagggg gagtaatcat ggttgcgttg atgggccctg
     2101 gcggaaccct caagatggcg gtgcgggcct tggaaagcgg gtgcgattcc gtgttcgtcg
     2161 gcccgaaagg gtggagccgc cgcccggcca cggatgaact tgcagacggc gagatccgcg
     2221 agctcatcga gtggagcgcg cctcgcggca aggatgtgcg catcgcaatc aacgtgatgc
     2281 ccgcccccga cgaaatcgat gtcttcctgt cgaaggtcga gcgatacgcc gcctggggcg
     2341 cgaacggcgt gatgatctgc gatcccggtt gcatcaagct cgtgcatgag cgtttgccgc
     2401 agctcgacat ccacgtcagc gtcacggccg gcatcttcaa tctgcgcgat atccagttct
     2461 atcgcagcct cggggccaat atcgtggtca tcccgtaccg ctggggcgta gcggagcttg
     2521 aggaaatccg tgaccagacg ggagttggcc tggaggcctt catgttccag agcatgaggc
     2581 gtggccgcat ctgtccgggc cgctgctatt cgagcagcta tttcaaaatc gcgcacacgg
     2641 ttgacgcgac gggcaagaac cacttcatcg gcagcgccag ccggggcgga agctgccatc
     2701 gcatctgccg ggcgaaatgg gatcttacgg cggactcgga gcttatgggg cagccggatt
     2761 tgaaagggac gcctgagctt ttgctctggg aaatgccgga ttacatcagg ctcggggtga
     2821 accgcttca
//
LOCUS       DATMGX010000537         2261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_131385, whole genome shotgun sequence.
ACCESSION   DATMGX010000537 DATMGX010000000
VERSION     DATMGX010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131385"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VNR65_08710"
     CDS             <1..1389
                     /locus_tag="VNR65_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ18793.1"
                     /translation="NGQAESVPVTVWDILSGLRPANAAAKENLCRVLDERDPSTVGPT
                     DALVLAAGMADDSVLVFSNAHRFWHDPAVAQGIWNLRDHFKSTGSMLVLLVAPGAALP
                     TELSQDVLVLDEPLPSADDLARIVKDTFAAAEIDEPAPDVGRRAVDALLGLSAFPAEQ
                     ALAMSLAKSGLDTEQLWERKRQVIEQTPGLSVWRGGESFADVGGCENIKRFLGAVLAG
                     EEAPRVVVFVDEIEKAFAGTGTDLSGVKTEMTGTMLSWMQDREADGVVLIGPAGTAKS
                     AVAKAAGTAAGIPTIAFDFGAMQNSLVGASGERLRSALQVVDAVSSGRSLWIATCNSI
                     GVLPPELRRRFTLGTFFFDLPTKEERATIWKIYLAKYGVAGARPDDEGWTGAEIKECC
                     RKAYRLRISLPESAQYIVPVSRSAAEQIKALRQQASGKFLSASQPGVYQIQDGAVLPK
                     AGRAFRDLESAG"
     gene            complement(1412..1813)
                     /locus_tag="VNR65_08715"
     CDS             complement(1412..1813)
                     /locus_tag="VNR65_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWJ18794.1"
                     /translation="MASVVADTHAIVWYLTNSPRLSPAAAKAFDDASAAGDSILIPSI
                     SLVELTYLVEKGRVPAEARKRLVDALAQPGGPYELAPLDGRVAAAVELIDRSVVPDMP
                     DRIIAATALSARAALVSRDGKIRASQVQTIW"
     gene            complement(1813..2040)
                     /locus_tag="VNR65_08720"
     CDS             complement(1813..2040)
                     /locus_tag="VNR65_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18795.1"
                     /translation="MTLEQAILDAVRALPANKQQELLDHALQLRDEATQPRKPRQSGR
                     GLWADLNIDLSAEAIDEARRESWKNFPRDDF"
BASE COUNT          416 a          631 c          778 g          436 t
ORIGIN      
        1 aacggtcagg ccgaaagcgt cccggtgact gtttgggaca ttctttccgg gctgcggccg
       61 gcgaacgccg ctgccaaaga gaacctgtgc cgggtgctcg acgaacgtga tccatcgacc
      121 gtggggccga cagatgcgct cgtgctggcg gctggaatgg ccgacgacag cgtcctggtc
      181 ttctcaaacg cgcaccgatt ctggcatgat ccggcagtcg cacaaggcat ctggaatttg
      241 cgtgaccact tcaaatccac gggttccatg ctggtgctac tggtggcgcc cggggcggcg
      301 ttgccaacag aactgtcgca agacgtactg gtgctcgacg aaccgctgcc ttcagccgac
      361 gatctggccc ggattgtgaa ggacacgttc gcggctgcgg agatcgatga accagcaccc
      421 gatgtagggc gaagagcagt cgatgccctt cttggtctct cggcatttcc ggctgagcag
      481 gcgcttgcga tgtcgctcgc gaagagtggc ctcgatacgg aacagctttg ggagcgaaag
      541 cggcaggtga tcgaacaaac tccggggctc agcgtgtggc gcggcggcga gtctttcgca
      601 gatgttggcg gctgcgagaa catcaagcgg tttctgggag cggtgctcgc tggtgaggaa
      661 gctccgcgcg tggtggtttt cgtggacgaa atcgaaaagg ccttcgccgg aacgggcacg
      721 gacctctcgg gcgtcaaaac ggagatgacc ggcacgatgc tgagctggat gcaggatcgg
      781 gaagccgacg gagtcgtgct catcggtcca gccgggacgg cgaagtccgc ggttgcgaag
      841 gcggcgggca cggcagcggg cattccaacg atcgcctttg acttcggagc gatgcagaat
      901 tctctggtcg gggcttcggg ggagcggctg cgctcggcgc ttcaggttgt cgatgctgtc
      961 tccagcggcc gcagtctgtg gattgcgacc tgcaattcca tcggcgtgct gccgcccgag
     1021 ctacgccggc ggtttacgct gggtacattc ttcttcgact tgccgactaa ggaagagaga
     1081 gctacgatct ggaagatcta cctggccaaa tacggcgtgg cgggagcgcg gccagatgac
     1141 gagggatgga ccggggctga aatcaaggag tgttgccgca aagcataccg gctgcggatc
     1201 tccttgccgg agtcggccca gtatatcgtt ccggtgtcgc gttccgcggc ggagcagatc
     1261 aaagcactgc ggcagcaggc gtccgggaag ttcctcagcg cttctcagcc aggcgtatac
     1321 cagattcagg acggcgcggt gttgccaaag gcggggcgag ccttccggga tctggagagc
     1381 gcgggttagg cggtgcaggt gttgcggctg gctaccagat cgtctgcacc tgtgacgcac
     1441 gaatcttacc gtcccggctg acgagggcag cgcgggcgga gagcgcggtt gcggcgatga
     1501 tgcggtccgg catatccgga accacacttc ggtcgatcag ctcgacagcg gcggctacgc
     1561 gcccgtccag aggcgccaac tcgtatggac cgcccggctg ggcgagtgca tccaccaatc
     1621 tcttccgggc ttcggcaggg acgcggccct tctcaacgag ataggtcagc tccacgaggc
     1681 tgatcgatgg gatgaggatg gagtcgccag ccgccgaggc gtcatcgaag gccttggccg
     1741 ccgcgggaga taatctcggg ctgttggtga ggtaccagac tattgcgtgg gtatccgcga
     1801 cgacggacgc catcagaagt catccctggg gaaattcttc caactttccc tgcgggcttc
     1861 gtcgatcgcc tcagcgctca gatcgatatt cagatctgcc cataggccgc gcccgctctg
     1921 gcggggcttc cgcggctgcg tcgcctcgtc gcgaagttgc agcgcgtgat ccaggagttc
     1981 ctgctgcttg tttgcaggaa gcgcccggac cgcatcgaga atcgcttgtt ccagagtcat
     2041 cggaaagccc acctgcaagt attatcggtt gtttttggcg ttcagaaaag gggggggatg
     2101 gcgggtcgaa aaggaggaaa atcgttttgt ccaaatacat ggagtgccag actgttatcg
     2161 gtgatcgcaa gcttcttgta gcggccctgg cggaactggg ctatcacgtg gaagtgcatc
     2221 cagagggtgc ggccttgttc ggctacgaag gccgcgagcg g
//
LOCUS       DATMGX010000538         2736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_249326, whole genome shotgun sequence.
ACCESSION   DATMGX010000538 DATMGX010000000
VERSION     DATMGX010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249326"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..965)
                     /locus_tag="VNR65_08725"
     CDS             complement(51..965)
                     /locus_tag="VNR65_08725"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HWJ18796.1"
                     /translation="MSYYEWHAYVPVAEKRRQAERKLAKLKKQGRSVAPVRIEGRTIT
                     KSFWGKSWCVNLERYSDYENRLPRGRTYVRNGSVIDLQIAKGEISAMVAGSELYQIKI
                     AIAPVTRARWKSICRDCAGTVDSLVELLHGRLAKGVMDRVCREGDGLFPSPTEIKLSC
                     SCPDWADMCKHVAAALYGVGARLDEKPQLLFVLRDVDENEMLASAGQDSPLTRAAPGA
                     AKVLDESDVAALFGLEMAETPRTPNPGFTIPKRPQQSKAQKSKISARKTKPTAKTDVA
                     LHRATRASSGTKKTTRASARKRRTRRRP"
     gene            complement(962..>2736)
                     /locus_tag="VNR65_08730"
     CDS             complement(962..>2736)
                     /locus_tag="VNR65_08730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWJ18797.1"
                     /translation="KAEIKRLLAHSDGLALIRGKWVEVDHERLNRTLEQFDAIERRAA
                     ADGLSFGEAMRMLAGAGIAGDKAAAPADIDWSQTVAGPWLAETLATLRHPDWLLRVDP
                     GQYFQGTLRPYQQSGVQWLYLLTQLKLGACLADDMGLGKTIQVLSLLLVIKNENRGNR
                     KPCLLVAPASLLANWAAEISRFAPSLKTVVVHPSAAPSQELNTYSAEYFADVDLVITS
                     YGFLTRTPWLETTSWRFVVLDEAQAIKNPAAKQTKSVKRLKAEMRIALTGTPIENRLG
                     DLWSIFDFINPGLLGSSKEFSTFVKRLADRPHNPYGPLRDLVRPYILRRLKTDKSIIA
                     DLPDKTEVKTFCPLSRKQAALYQQAVAELAGHLEEVDGIKRRGIVLAFLMRLKQICNH
                     PSQWLGDGAWDEEDSGKLGRLRDIAEVVAARQEKALVFTQFKETTAPLVAFLGSVFGR
                     PGLVLHGETEVKKRKDLVRQFQEDEDVPFFVLSVKAGGTGLNLTAASHVIHFDRWWNP
                     AVENQATDRAFRIGQTKNVLVHKFVCRGTVEEKIDHMIESKKQLAGDFLGGGADILLT
                     EMKDEELLKLVSLDLGAAMKEGDE"
BASE COUNT          547 a          820 c          747 g          622 t
ORIGIN      
        1 gatctaccta gcggcgaaac ggtcactcgt ggatgcttgt tcacagaata ctatggtcgc
       61 cgccgggtgc gccgtttcct ggcacttgct cgggttgtct ttttcgttcc cgaactggcc
      121 cgtgtcgcac gatgtaaggc gacatcggtc ttcgctgttg gcttggtctt tctggcagat
      181 atcttgcttt tttgcgcctt ggactgttgg ggtcgctttg ggattgtgaa gccaggattt
      241 ggggtacggg gagtttcagc catctccaga ccgaatagag ctgcgacatc gctttcatca
      301 agcaccttcg ccgcgcctgg cgctgccctt gttagcggag aatcctgtcc agcgctcgcg
      361 agcatttcat tttcatccac atcgcgcagc acaaaaagaa gctgaggctt ttcatcaagc
      421 ctggcgccga caccatacag tgccgccgcg acgtgcttgc acatgtctgc ccaatcggga
      481 cagctgcagg acagcttgat ttccgtcggc gacggaaaca ggccgtcacc ctctcgacag
      541 acccggtcca tgacgccttt cgccaagcgg ccgtgcaaga gctcaactag cgaatcgacg
      601 gtcccggcgc agtcccgaca gatggatttc cagcgtgccc ttgtaaccgg tgcaatggca
      661 atcttgattt ggtagagctc agacccggcg accattgccg agatctcgcc cttggcaatt
      721 tgcaggtcga taacggagcc gtttcggaca taagtccgac cacgcggcaa gcggttctca
      781 tagtcgctgt atcgttcaag gttgacgcac caagatttac cccagaagct cttggtaatt
      841 gtgcggccct cgatcctcac gggcgcaacg gatcgtccct gcttcttgag cttggccaac
      901 ttgcgttctg cctgccgccg cttctcagct actggcacgt aagcgtgcca ctcgtagtag
      961 ctcattcatc cccttccttc atcgccgcac ccaaatcgag actcacgagt ttaagcaact
     1021 cctcatcctt catctcagtc aacaggatat cagctccgcc gccgagaaaa tccccagcca
     1081 attgtttctt cgactcgatc atatggtcga tcttttcctc cacggtgccg cgacaaacga
     1141 acttgtgcac aagcacattc ttggtctgtc cgatccggaa ggcacggtcg gtggcttggt
     1201 tttcgacagc cggattccac cacctgtcga aatgaatgac atgcgaggcc gctgtgagat
     1261 tgagcccggt gccgcctgct ttgaccgaga gcacgaagaa cgggacgtct tcgtcttcct
     1321 gaaattggcg caccaggtcc ttgcgcttct taacctctgt ctcgccatgc aggacgaggc
     1381 cgggccttcc gaacaccgag cccaggaacg ccactagtgg cgccgttgtc tccttgaact
     1441 gggtgaagac aagtgccttc tcctgccggg cggcaaccac ttccgcgatg tcgcgcagac
     1501 gcccaagctt accgctatcc tcctcatccc acgcgccgtc gccgagccac tgcgatggat
     1561 ggttgcagat ctgcttcaaa cgcatgagaa aagctaggac gatgcctctc cgttttattc
     1621 catcgacctc ttcgagatgg ccggccagct ctgccactgc ttgctgataa agcgccgcct
     1681 gcttacggct caaggggcag aaggttttca cctcggtctt gtcgggcagg tcggcaatga
     1741 tgctcttgtc ggtcttcagc cgtcgcagga tataggggcg caccaggtcg cggagcggac
     1801 cgtaagggtt atgtggccgg tcggcgaggc gtttgacgaa tgtcgaaaat tccttcgacg
     1861 atcccaacag gcccggattg atgaagtcga agatcgacca taggtcaccg agccgattct
     1921 caatgggcgt gccagtcagc gcaatgcgca tttcggcctt gagcctcttg accgattttg
     1981 tctgcttagc agccgggttc ttgatggcct gtgcttcatc gagcaccaca aatcgccacg
     2041 atgtagtttc tagccaaggc gtacgcgtca gaaagccgta gctcgtgatt acgagatcga
     2101 catctgcgaa gtattctgca ctgtaagtgt tcaattcctg ggacggcgca gccgatggat
     2161 ggaccacgac tgtttttaag cttggcgcaa atcgggaaat ctcggcggcc caattggcta
     2221 acaacgaggc gggggctacg agcaggcaag gtttgcgatt tcccctgttc tcattcttga
     2281 tcaccagcag cagcgacaac acttggatgg ttttgccgag ccccatatcg tcggcaaggc
     2341 aagccccaag cttgagctgt gttagcagat agagccattg cacacccgac tgctgatagg
     2401 gtcgcagcgt accctgaaaa tattgaccgg ggtcgacccg caatagccaa tcgggatggc
     2461 gcaaggtcgc cagcgtctcc gccagccagg ggccggcaac ggtctggctc caatcgatgt
     2521 cggctggtgc ggcagcttta tctccggcaa tgccagctcc agccagcatt cgcatcgcct
     2581 caccaaacga aagaccatcg gcggcggcgc ggcgctcgat ggcatcaaat tgctcgagtg
     2641 tgcgattcag ccgttcatga tcgacctcaa cccacttgcc gcggattaag gccaatccat
     2701 cggaatgcgc caggagtcgc ttgatctcgg ccttcg
//
LOCUS       DATMGX010000539         2270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_355,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000539 DATMGX010000000
VERSION     DATMGX010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VNR65_08735"
     CDS             <1..618
                     /locus_tag="VNR65_08735"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HWJ18798.1"
                     /translation="SSGLAHHKLDMNRQLRAEELPGIFSSHVSDVWLEIGFGAGEHLI
                     WQAERNPHAGLIGAEPYVNGVVAALSALEARRLGDRIKIHPDDTISLLNWLPEASLSR
                     AFVLFPDPWPKKRHRERRIFAPALLEKLGRVLRSGAEIRFASDIADYAEAAIEHANAH
                     PDFEVVGIFTSEYRATASDWPVTRYEEKASKQGRSSTFISMRRLA"
     gene            complement(649..>2270)
                     /locus_tag="VNR65_08740"
     CDS             complement(649..>2270)
                     /locus_tag="VNR65_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18799.1"
                     /translation="LAKFARFAGYAQLQDFERELRQVLETVQKHYAALFESTPELTTS
                     RGGNLVFTGDADDPATSETLVKLGFKNASAAIAAIKGWHYGRYPAMRSARARETLTEF
                     TPALLEALGHTGDPDLALATFDKFLSEVPTALQLFAMLRANPNLLQLVAQIMGSAPRL
                     ARLLTRRRRIMDVILDPGHLGDKPTDEELRRHIRQETLDAGAYEDRLNRSRVVGQEQM
                     FLIGVQLLSGQLTPLQAGDAYSLVAEAIIESLLDAVQAHYGGALPAPAVIAMGKLGGH
                     ETTPSSDVDLIVIYDAPASTPQASQHYARITQRLISAISAPTSEGDLYAVDMRLRPSG
                     KAGPVAVRLDGFLSYQRNEAWTWEHLALTRARPIAGPPEVREHLRQIIREVLTTPRDR
                     EKAAADVREMRAMIEKEKGTSDIWQTKAYKGGLVDVEFIAQFLQIVHAAENPGILDQN
                     TANALQRLIAAGFLSAPDGDALLKASALYQNISQIVRLCTEGSFDPRDAPRDLVDLLL
                     HTTGEPDLSRLEARLCETYAEVACLFLKLVV"
BASE COUNT          448 a          677 c          702 g          443 t
ORIGIN      
        1 tcatcgggct tggctcatca caagcttgac atgaaccggc aactccgtgc ggaggagctg
       61 ccgggcattt tttccagtca tgtcagcgac gtctggcttg aaatagggtt cggcgcggga
      121 gagcatctga tctggcaggc ggaacgcaat ccgcatgctg ggctcatcgg cgccgagccc
      181 tacgtcaatg gagtcgttgc cgcgctctcg gcgctggagg cacggcggct tggcgaccga
      241 ataaagattc acccggacga taccatttcg ctcttaaact ggcttccgga ggcgagcctt
      301 tcgcgtgcct tcgtgctttt tcccgacccc tggccgaaaa agcgccatcg ggagcgccgc
      361 atctttgcgc ccgcgctgct tgagaagctg ggccgtgtgc tgcgctccgg cgcagaaatc
      421 cgcttcgcct cggatatcgc agattatgcg gaggctgcca tcgaacacgc gaacgcccat
      481 ccggatttcg aggtcgtggg aattttcacc tctgaatatc gcgcgacggc atccgactgg
      541 cccgtgacgc gctacgaaga aaaggcgagc aaacagggcc gctcatccac gttcatctcg
      601 atgcggcggc ttgcttaacg cgtctggaaa cggatcggcg attcagtctc agacaaccag
      661 cttcaaaaag agacaggcga cctccgcata agtctcgcaa agccgtgctt caagccggct
      721 cagatccggc tcgccggtgg tatgaagcag gagatcgaca aggtcgcgtg gagcgtccct
      781 gggatcgaag ctcccctccg tgcagagccg cacgatctgg ctgatgttct ggtaaagcgc
      841 ggaggccttc agaagcgcat ccccgtccgg cgcgctcagg aagccagccg cgataagcct
      901 ttgcagcgcg ttcgccgtat tctgatcgag gatgccgggg ttttcggccg cgtgcacgat
      961 ctgaaggaac tgagcgataa attcgacatc gaccagacca cccttgtagg ccttcgtctg
     1021 ccagatatcg cttgtgcctt tttccttttc gatcatggcc cgcatctcgc ggacgtcggc
     1081 ggcagccttc tcccgatctc tcggcgtcgt cagaacctct cgaatgatct gccggagatg
     1141 ctcgcgaacc tccggggggc ctgcaatggg ccgggcgcgc gtcagcgcaa gatgctccca
     1201 ggtccaggcc tcgttgcgct gataggacag gaagccatcg aggcgaacgg ccaccggacc
     1261 cgctttcccg gatggcctca aacgcatatc gacggcatag agatcgcctt ccgaggtcgg
     1321 agcggaaatg gcgctgatca accgctgggt gatgcgcgca taatgctgcg aggcctgcgg
     1381 cgtcgaggcc ggcgcatcat aaatgacgat gagatcgaca tccgaggaag gggttgtctc
     1441 atgtccgcca agcttcccca tcgcgatgac ggcaggtgcg ggaagcgcgc cgccatagtg
     1501 agcctgaacc gcatcgagca ggctctcgat gatggcttcc gcgacgagcg aatatgcatc
     1561 gcctgcttgc agaggagtga gttggccgga aagaagttgg acgccgatca ggaacatctg
     1621 ttcttggccc acaacgcgtg agcgattgag gcggtcctca taagcaccgg catccagggt
     1681 ctcctgtcgg atgtgccggc gtagttcctc atccgtgggc ttgtcgccca gatggcccgg
     1741 gtcgaggata acatccatga tgcgcctgcg ccgggtgagg agccgggcca gtcttggcgc
     1801 ggaccccatg atttgcgcga caagctgaag gaggttggga ttggcacgca gcatagcgaa
     1861 aagctggagc gcggtcggaa cctcgctcaa aaatttgtcg aaggtcgcaa gcgcgagatc
     1921 tggatcgcct gtatgtccaa gcgcctcgag gagggcaggc gtaaactcgg tcaaggtctc
     1981 ccgagcccgg gcgcttcgca ttgccgggta gcgcccgtaa tgccaccctt tgattgcggc
     2041 gatggcagcc gaggcattct tgaagccaag ttttacaagc gtctccgaag ttgcaggatc
     2101 gtccgcgtcg ccggtgaaaa cgaggtttcc gcctcgcgac gttgtcagct ccggcgtgct
     2161 ctcaaacaga gccgcgtaat gcttctgcac ggtttccagc acttgccgaa gttcgcgctc
     2221 gaaatcctga agctgcgcat aacccgcaaa ccgcgcaaat ttcgcaaggt
//
LOCUS       DATMGX010000540         3157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_1053,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000540 DATMGX010000000
VERSION     DATMGX010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..596
                     /locus_tag="VNR65_08745"
     CDS             327..596
                     /locus_tag="VNR65_08745"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HWJ18800.1"
                     /translation="MMRNLFRAAAVVGTVASIGMTPSPVQARDYPFCIKGDFYDSPIG
                     DCSFDTYEQCLATASGRKAYCDANPFFAYPKKQQFDYPKKQRGQY"
     gene            complement(1733..2332)
                     /locus_tag="VNR65_08750"
     CDS             complement(1733..2332)
                     /locus_tag="VNR65_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18801.1"
                     /translation="MTRAKNQVAPPAKLPLLITESADEFAVLRAKLQQEIEPNGIIEQ
                     IYLDDLAVIVWEIKRLRRSKTGIINNAFRSALQSLLRQLLLTPSNFNRPDTEWTADAL
                     ADGWFTSQRTRRVVLRILHRYHLDESAIEAEAIRLCSSDLEQLDRMLTSLRLRFDRVL
                     RCVADYREALAKRMRQSSDRVLEDADVLRLESFADKKSA"
     gene            2494..2946
                     /locus_tag="VNR65_08755"
     CDS             2494..2946
                     /locus_tag="VNR65_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18802.1"
                     /translation="MLLPCPVNALILGKMIGARGQASLCRRIFRTPVSEMNLRHKRHC
                     AGGDHHAKNAAARCFRLVLESQENEYWLICTASRMRSYDNVFKRMLAIREIFRRIKRM
                     ALVKAGFPAFDLPQTIESSGKLKNISAKNANGCDATEGVPAQTMAMGR"
BASE COUNT          663 a          838 c          905 g          751 t
ORIGIN      
        1 atgctccact cgtcgatgtc tggtgtgact ggtcctgtct ctatagttgc gactggctcc
       61 accaaaccgc ccggcgcgtc ctacgagttc aaggaccagg gcacactcgt tggctcgatc
      121 cgcgctagtc gcaccttctg atagctgtcg tcccaggacg accacaatct gagatcagaa
      181 cccccggcag gaaactgccg ggggtttttc tttgcaagat caagcacagc gctgcggcgc
      241 cctgtatacg ttttacgttt tcagaacggt tgacgcggaa cgtttcttgg agtcccgcgg
      301 tccatggcgc cccatcaggg aggtcgatga tgcgtaatct tttccgggca gccgccgttg
      361 tcggcaccgt ggcttcaatc ggcatgacac catcgccggt tcaggccagg gattatccgt
      421 tctgcatcaa gggtgacttc tacgacagcc cgatcgggga ttgcagcttc gacacctatg
      481 agcagtgtct ggcgacggcc tcgggccgta aggcgtattg cgatgcgaat ccattctttg
      541 cttatccgaa gaagcagcaa tttgattacc cgaagaagca gcgcgggcaa tactagaggg
      601 tccggactca taaccagtag ccgaatgtgc agcgatgcag acgactgcga ggaggttatt
      661 cgcgtcgcgg tggtatgagt tgcgatgcga cgaaagtcct tgacgcggcc gaacattttg
      721 gcggcagatg tgggcagttc gtcgttgcgc aacctacaac gccagcaacg agctgccgag
      781 cgaatacgtc tcgaacttcc agtacaactc ggactaacta gactcccggc aagaacctcg
      841 cgttctcggc tgaagcctgt ggatggggtc tcgaccagga gttcactggc ggggccggat
      901 ttactccgac ggcccccaat ccaacgacct ttacgagtac aaggaccaaa agctgtgtcc
      961 taccccgagc tggtgttcag gaatgagttc aaggacgagg gcactgtatc gctccagctt
     1021 cgcgctcagt gtaaccttct gggcgtatcg tctaacggca atcctccgaa tttagagctg
     1081 atccaagtga ttgtatttac gtcgtaatat tggcttcgtt ttgcggaatt gccatttaac
     1141 aacttatagt tttaacaatc taaagcacgt ctctttgtaa tggcgcggat agcccgatcc
     1201 cgacgggcgg cggcgcggca ttcgtatcgg tcaagctgga ttaggttagg gagtatacgc
     1261 cgaaccgctt cagacaagcg ttgcggttcc agcgacggca gcgtcgctga cgggtctttc
     1321 aatttcggcc gtgggggtct cgtacccttc ttttccgaca tccagagatc cattgcgatc
     1381 aaccaacgga cttcttgaac ggcagagggg aagtgctttg gagcctcgag acttccgagc
     1441 gcggatgcgc gttcgaacag ggcgagtttg actcgcctga tgcgggcaag ttcaagttct
     1501 gcctccgcag cgctgcgggc gtgctcgagg gtgatggtat ccctcgtcgg acctgcgatc
     1561 ttgcgggcga gctcgtcgac ttgttttgca aacgccgtgc ttgactgact taagttcagt
     1621 ccgtgggtat acgcattccg gcttgcgcgc tttttcccgg cgccggaacg ggggccggtg
     1681 ctttttctcg cgttccgccg gtttgcctcg atttgtcttt cacttgccat ggtcaagccg
     1741 acttcttgtc cgcaaagctt tcgaggcgaa gcacatcagc gtcttcaaga acccgatctg
     1801 aactctgacg catccgtttg gcaagagcct cccggtaatc ggcgacgcag cgaagcaccc
     1861 tatcgaagcg caatcggagg gaggtcagca ttctgtcgag ctgttcaaga tctgacgagc
     1921 acagtcggat cgcttccgcc tcaatggctg actcgtcgag gtgatatcga tgcaggattc
     1981 tcaaaaccac ccttcttgtc ctttgactgg tgaaccagcc gtcggcgagc gcgtcagcag
     2041 tccattcggt atccggccgg ttaaagttac tgggggtcaa caacagctgc cggagcaggc
     2101 tttgcagcgc ggagcggaag gcgttgttga tgatgccggt tttgctgcgt cgcagtcgct
     2161 tgatctccca gacgatcacc gccagatcgt ccagatagat ttgctcgatg atgccgttgg
     2221 gctcgatctc ttgctgcagc tttgcgcgca agactgcgaa ttcgtcggca gattcggtga
     2281 tgagcagagg cagtttcgct ggcggtgcca cctggttctt tgcccgggtc atctctggat
     2341 ccctccggta ttctgcatgc tgtccgaagt tgaacgcata acccggagcg aactgctggc
     2401 tttgcctttg ccctcgatca agggcggttg atcttctggt ttgtttgacg acatttgcgt
     2461 ttctccaaaa atagggccaa gagcgaggca cacatgctgc tgccttgccc tgtgaatgct
     2521 ttgatcctgg ggaaaatgat cggcgcgcga ggacaagcgt cattatgccg aagaattttc
     2581 cgtacgcctg ttagtgagat gaacctgcgc cacaaaaggc actgtgctgg tggcgatcat
     2641 cacgcgaaga acgccgctgc gcgatgtttc aggctcgtac tggagtcgca ggaaaatgaa
     2701 tattggttga tttgcactgc gtctcggatg cgaagttacg acaatgtttt caaaagaatg
     2761 ttagcgatcc gagaaatttt tcgcaggatc aagcgaatgg ctttggtaaa ggcgggattt
     2821 cccgcattcg acttgccgca aacaattgaa tcatcgggta aattaaaaaa tattagcgca
     2881 aaaaatgcga acggctgtga tgcgacggag ggtgtgccag cgcaaacgat ggcgatgggt
     2941 cgctgacaat accatggtgt cctgaaggat tcggttgttc ctcgaagcct tggataaccg
     3001 agacgatctc gaaccgtacg atttgtgcag gcagcgatgc tgcaaacgac tggttcattt
     3061 gatctttaga atcacaacaa gtgccaaaaa atattggcgc gacagtccca ggacgctggc
     3121 ggtgtgcaaa ctgccggccc tggcagatcc tcagtct
//
LOCUS       DATMGX010000541         3806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_301091, whole genome shotgun sequence.
ACCESSION   DATMGX010000541 DATMGX010000000
VERSION     DATMGX010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301091"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..693
                     /locus_tag="VNR65_08760"
     CDS             286..693
                     /locus_tag="VNR65_08760"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HWJ18803.1"
                     /translation="MLLEWNENLSVGVPSIDEQHKALLGLLNELFDATEAGRGQLVLG
                     KVLKELADYTVYHFKYEESLFAQTGYSAAPDHVKEHNDLTRLVQVQRQKYEDGASEIL
                     SEELVKFLRRWLYMHIMGSDKKFGPHLVSMGIK"
     gene            748..2253
                     /locus_tag="VNR65_08765"
     CDS             748..2253
                     /locus_tag="VNR65_08765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWJ18804.1"
                     /translation="MERLKITMAIRLLLALGTISLFSLSGIGLWSLRSQMLEDRQAEL
                     RKLLDLTLSVARAAMIKAGGAESEAGRNTFFEVLQSARFGKDKEQNYIFAYDYDGFAK
                     AHADLKRLGHNRINVVYANGVKVIKEFIDIARSPAGFGFIEYPVEKGVGGPFKPKLSL
                     IQNVPEIGGLVGVGAYIDDVDADTYRELFIEGAMLAAVLAAIGAAGFLIGRMLMTVTS
                     EMEHQLAAAIALQSDMLPSSEQLAEIQGGCPLDLSSYYKPLDGIGGDIWGTEVIGPQR
                     IMIYIADFTGHGVSAALNTARFHSFVHTELQGTDEPALLLQRLNNRLHAVLPTGQFAT
                     AFCAAIHFDMQTIECVSAGAPPQLYRRSSEDQFEVISQPSLPLGIISDVAYESRTVAF
                     YPGGSLVLYTDGLIETPKPPRSLFTTESLRERLNKTSQATSLQLCQSILDEFSKSAIR
                     ADDDITLVIAKYTGGVWNLSMKSDAPALKVLAASDSDQGHQDCGRRCKLRC"
     gene            complement(2533..>3806)
                     /locus_tag="VNR65_08770"
     CDS             complement(2533..>3806)
                     /locus_tag="VNR65_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWJ18805.1"
                     /translation="SIKYALMAGMLAGGAEVHDIGLALSPTAYFAQFALEVEGVAMVT
                     ASHNDNGWTGIKMGMARPLTFGPEDMSALRDLVLEGRFALHEGGSYHFHADIAEGYTA
                     DLTNREPFKRKLKVVAACGNGTAGAFAPQVLEKLGCEVIPLNTDLDYTFPNHNPNPES
                     LKMLHALSDAVRLSGADVGLAFDGDGDRCGVVDNEGTEIFADKVGVMLARDISANNAN
                     AKFVVDVKSTGLFTTDPVLRKNGAQTAYWKTGHSYIKRYSHETGALVGFEKSGHYFFN
                     PPLGRGYDDGLISALAVCDMLSHNPSKSMADLYRELPKTWQSPTMSPHCADEKKYGVV
                     DRVIAHFKGIAERGEKLAGQPIRDVITVNGIRIMLEDGTWGLVRASSNSPELVVVVES
                     PASEANMRAIFAELDAHLSTYPEIGEYNQKI"
BASE COUNT          808 a         1057 c         1097 g          844 t
ORIGIN      
        1 tttccaaaac tgccaacatc ccgccgttgt ccctcaggtc ataagcacga gtgtggcgcg
       61 gcggctccgt ttcaagcccc ttgacagaaa tgccatgagc gccattcgca ggcgcctgcg
      121 agttcccggt ccacggcggc aatcttaggc tttctcttaa ccataccaag acataccgca
      181 catatactcg cttgggttgc acgtctgttt ttggagatgg atcggatgcc gaacgcgctt
      241 gctgcccagg cgcgggccaa gaagtgatat gtaacggagg cggcgatgct tttggaatgg
      301 aatgaaaacc tgtcggttgg cgtgcccagc atcgatgagc agcacaaagc gctgttagga
      361 ctgctcaatg aactcttcga cgccaccgag gctgggagag gtcagctcgt gttgggcaag
      421 gtgcttaagg agcttgccga ctacacagtt tatcatttta agtatgagga gtccctcttt
      481 gcccagacag gctatagtgc tgcaccggat cacgtgaaag aacataacga cctgaccaga
      541 ctggttcaag tacagaggca gaagtatgag gacggcgcca gcgaaattct gtctgaagag
      601 cttgtgaaat tcctgagaag gtggctgtac atgcacatca tgggcagtga caagaaattc
      661 gggccgcact tggtctcaat gggaataaag taattttcgg cgacctttct ggcgtcgatg
      721 gcgtgagggg cctggagcgt tgcgtgcatg gagcggctta agatcacgat ggccatccgt
      781 ctcctcctgg ctctggggac aataagcctt ttttcattgt cggggatcgg gctttggtcg
      841 ctgcgttcgc aaatgcttga ggaccggcaa gccgagcttc gaaagctcct ggatttgacc
      901 ctctcggttg ctcgcgccgc catgattaag gccggaggag ccgaaagcga ggctggacgg
      961 aatacgttct tcgaggtcct ccaatcggca aggtttggca aagacaagga acaaaactac
     1021 atttttgctt atgactatga tggctttgca aaagcccacg ccgatctcaa aaggctgggg
     1081 cacaaccgta tcaatgtcgt ctatgcgaat ggcgtaaaag ttatcaaaga gttcattgat
     1141 atcgcaagaa gtccagctgg gttcggattt attgagtatc ccgtcgagaa aggagtgggc
     1201 ggtccgttta agcccaagct ctcccttata caaaatgtgc ctgagattgg aggacttgtt
     1261 ggcgtcggcg cctatatcga tgatgtggac gcggacactt accgtgagct tttcattgaa
     1321 ggcgctatgc ttgccgcagt tctcgcagcg atcggcgctg caggctttct aatcgggcga
     1381 atgcttatga ctgtgacgtc cgaaatggag caccagcttg cggctgcgat cgccctccag
     1441 agcgacatgc ttccgagttc ggagcagcta gcggaaattc agggtggctg tcctttggac
     1501 ctttcgagct attacaagcc gctcgatggt atcggggggg atatttgggg cacggaagtt
     1561 atcggccctc aacgcatcat gatctatatc gcggatttca cagggcatgg cgtttcagcc
     1621 gctcttaata ccgctcgttt ccattccttt gtccatacgg aactccaagg cacagacgag
     1681 cccgctttgt tgctgcaacg attgaacaac aggctgcatg ccgtgctgcc taccggccag
     1741 tttgcaaccg cgttctgcgc ggcgatacat ttcgacatgc aaacgatcga atgtgtctcg
     1801 gcgggcgccc ctcctcagct ctatcgcagg tcgtctgagg accaattcga agtcatctcg
     1861 cagccaagct tgccgctcgg catcatctcc gacgtggcct atgaaagccg aacggttgcg
     1921 ttttatcccg gcgggagtct tgttctctac accgatgggc tgatcgagac gcccaaaccg
     1981 ccgcggagcc tatttacgac ggagtctttg agagagcgtc tcaacaagac cagccaggcc
     2041 acctcgttgc aactgtgcca gagcattcta gacgaatttt caaaatcagc gatcagagca
     2101 gacgacgaca tcactctcgt tatcgcgaag tataccggcg gagtctggaa cctctcgatg
     2161 aaatctgacg ccccagcttt gaaggttctc gccgccagcg attccgatca aggccaccaa
     2221 gattgcggac gaaggtgtaa acttcgctgt tagcccacga cgtcaaatag agattgtcgg
     2281 ctaatggccc agattgcctg gagctgggac caccacaaaa catccgctaa aggatctgaa
     2341 agcaaacgtt gccgctcgaa caatcttcga ggcccgctat ttctgcaaac ggcctgttgc
     2401 ggagatactg cgcatagcgc tcgatctcac aagatgttta tgcccatggc catccgtggc
     2461 taaaattact tacatgttta cttatggcca ctttaccaaa cgaacccaat ctggttaatc
     2521 cgatgtagac actcagatct tctgattata ctcgccaatc tctggatagg tggagagatg
     2581 ggcgtcaagc tccgcgaaga tggcgcgcat gttcgcctcg gaggcggggc tttccaccac
     2641 gacgaccagt tccgggctgt tggacgaggc gcgcaccagc ccccacgttc cgtcctccag
     2701 catgatgcgg atgccgttca cggtgatgac atcgcggatc ggttgccccg ccagcttctc
     2761 gccccgttcg gcgatgccct tgaaatgcgc gatcactcgg tcgactacgc catatttctt
     2821 ctcgtcggcg caatgcgggg acatggtggg cgactgccag gttttgggca gctcgcgata
     2881 aaggtcggcc atgctttttg aggggttgtg cgagagcatg tcgcaaacgg cgagcgctga
     2941 gatgaggccg tcgtcatagc cgcggccgag cggcggattg aagaagtagt ggccggactt
     3001 ctcgaacccg acgagcgcac ccgtctcgtg gctatagcgc ttgatgtagg agtggccggt
     3061 cttccagtag gcggtttggg cgccgttctt ccgcaaaacg gggtcggtgg tgaaaagccc
     3121 cgtcgatttc acatccacca cgaatttcgc gttcgcgttg ttggccgaga tatcgcgagc
     3181 cagcatcacg ccgaccttgt ctgcgaaaat ctcggtgccc tcgttgtcca ccacgccgca
     3241 ccggtcgccg tcgccgtcga aggcaagccc gacatccgcg cccgagagcc tcaccgcgtc
     3301 cgagagcgcg tgcagcatct tgagggactc ggggttcgga ttgtggttcg gaaaggtgta
     3361 gtcgaggtcg gtattgagcg ggatcacctc gcagcccagc ttttcgagca cctgcggcgc
     3421 gaaagcgccc gctgtcccgt ttccgcaggc cgccacgacc ttgagcttgc gcttgaacgg
     3481 ttcgcggttc gtgagatcgg cagtgtagcc ctcggcaatg tctgcgtgga agtgataaga
     3541 gccgccctca tgcagagcga agcggccttc gagaacgagg tcgcgcagcg cgctcatgtc
     3601 ctccggtccg aaggtcagcg ggcgcgccat gcccatcttg atgccggtcc agccattgtc
     3661 gttgtggctc gccgtgacca tcgcaacgcc ttcgacttcg agcgcgaact gcgcaaaata
     3721 ggccgtgggc gagagcgcca gcccgatgtc gtgcacctcc gcgccgccag caagcatgcc
     3781 ggccatcaac gcatatttga tgctgg
//
LOCUS       DATMGX010000542         2873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_33857, whole genome shotgun sequence.
ACCESSION   DATMGX010000542 DATMGX010000000
VERSION     DATMGX010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33857"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VNR65_08775"
     CDS             complement(<1..775)
                     /locus_tag="VNR65_08775"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWJ18806.1"
                     /translation="MDGRLARRFQQSHRFPVRSTAAQAMRSVPTIVAQSSRARPRLRI
                     PWRALRVVCSGAALLWIVRTTPLNAISAILHRADWRWLISGFALNLLARVAAAERTLV
                     ISRALGLRVSRRQTLQTLFISNYYALLSPGPVLSGVVSVYRYKSQGATLTGSISTLLG
                     SRAVEAAIFIVIGATCVLIDSRISLTAVRVPLSLALVAGILFGIALTGWSLLHRRQAG
                     LRVAGIPDKPGGGGRFSQILAVWHVLLRHGPLMAFRAAVP"
     gene            complement(720..2060)
                     /locus_tag="VNR65_08780"
     CDS             complement(720..2060)
                     /locus_tag="VNR65_08780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ18807.1"
                     /translation="MISRNLPALPPVDWTGPQANFSPNRKIRPLLRDRHGVNRGRVLI
                     VTPQPFYEDRGTPIAVRYVAGALSDLDVDVDLLAFPIGESIRIKRMRVYRCANHLGLR
                     RVPVGLSWRKLLLDVSLWRSFRQLLATRRYDMVHAVEEAAYMASVLCPKFAQPYIYDM
                     ASCIPNALRRNASLNFSPLMRLFRASEQRVFSGASHTICSAGLESYVRERASTASVAE
                     WRFPAHAGAVSQADVATLRVSLGIQSDQTVLLYTGNFAAYQGIDLLIDAFTRACLMRP
                     NLILLCIGATDQELTERRQTATLGGQEHIRFLPRQPRDRMLTYTAMADALMLPRIGPQ
                     NIPLKLYDYMASGRPIVAMRQRGSESMLTDERAFLCAPTAESMARAIVRICEHPDEAA
                     LVGGEALRYAQRYFGWGEFVEFTRGIYETAIGDRSQVGRPARPPLPTIASISGS"
     gene            2303..>2873
                     /locus_tag="VNR65_08785"
     CDS             2303..>2873
                     /locus_tag="VNR65_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ18808.1"
                     /translation="MPTHILLIADDAAFATAAVDALPATGSVCYSFEWVRRCADGVDR
                     LDRDSKDTSSRLDPIAAVMVDLALPDSVGMTAFDQVFHATGNIPILVLSQASDEEIAR
                     QTLQHGAQDYLIKERMDSYSLRKALSGVIERAAASRELNSENERAQVTLNSIGDAVIS
                     TDAVGHVTYLNSVAERLTGWSQAEAADRQL"
BASE COUNT          624 a          856 c          885 g          508 t
ORIGIN      
        1 ccggaacggc cgccctgaag gccatcagtg gtccgtgccg cagaagtacg tgccaaaccg
       61 caagtatctg gctgaatcgg ccgccaccac cgggtttatc cggaatacct gcgacccgga
      121 gacccgcctg acgccgatgc agcaaggacc agcccgtcaa tgcgataccg aacagaatcc
      181 ccgcgaccag cgccaggctg aggggaacgc gcacggcggt caacgaaatc ctgctgtcaa
      241 tgagcacaca tgttgcgccg attacaatga aaatcgcggc ttcaaccgca cggcttccga
      301 gcagcgtgct gatgctgccg gtgagcgtcg ccccctgact cttgtaccgg tagacgctga
      361 cgacgccgct caatacggga cccggcgaca gcagcgcata gtagttgctg ataaacagcg
      421 tctgcagcgt ttgtcggcgg gaaacgcgca gtccgagcgc ccggctgatc acgagggttc
      481 gctcggcagc ggcgacgcgc gcaagaagat tgagggcgaa gccagaaatc aaccaccgcc
      541 agtcggcacg atgcaagatt gccgagatcg cattgagggg cgtagtacgg acgatccaca
      601 aaagcgcggc gccgctgcac acgacgcgca gcgcacgcca cggaatacgc agcctcggcc
      661 gtgcgcgcga ggactgggcg acgattgtag gcacggatcg catggcttgg gcggccgtgc
      721 tacgaaccgg aaatcgatgc gattgttgga agcggcgggc gagccggccg tccaacctgg
      781 gaccgatctc caatcgcagt ctcgtaaatg ccgcgcgtga attcgacgaa ttcgccccag
      841 ccgaaatatc gctgcgcgta gcgcaatgcc tcgcctccca ccagcgcggc ttcgtccggg
      901 tgttcgcaaa ttctgacgat tgcgcgcgcc atggactcag cagtcggggc gcaaagaaag
      961 gcgcgctcat cggtgagcat gctctcggaa ccacgttgcc gcatcgcgac gatcggtctt
     1021 ccggacgcca tatagtcata gagtttcaat ggaatgttct gcggaccgat gcgcggcaac
     1081 atcaacgcat ccgccattgc cgtatacgta agcatgcggt cacggggctg ccgcggcagg
     1141 aaacggatgt gttcttgacc cccaagggtc gcggtctgcc gtctctcggt cagctcctga
     1201 tcggtcgcgc cgatgcataa caggatcaag ttcggtctca tcaagcaggc gcgggtaaaa
     1261 gcatcgatca gcaggtcaat gccttgatag gctgcaaaat tgccggtgta caaaagcacc
     1321 gtttggtcgc tttgaatacc cagactgacc cgcagcgtcg caacatcggc ctgggagacc
     1381 gcgcccgcat gagccgggaa acgccattcc gcgaccgaag cagtggatgc ccgttcgcgg
     1441 acataggatt ccagcccggc gctgcagatc gtgtgactgg cgccggaaaa aacgcgctgc
     1501 tctgacgctc ggaacagacg catcagaggg ctgaaattga gcgaggcgtt gcgtcgcaag
     1561 gcgttgggaa tacaggacgc catgtcatag atatagggct gcgcgaattt cggacacaac
     1621 acggatgcca tataggccgc ttcctcaacc gcatgaacca tgtcgtagcg ccgtgttgcc
     1681 aggagttggc gaaaggaacg ccataaactt acatccagaa gcagctttcg ccaggataat
     1741 ccgacgggca cgcgcctcag gcccaaatgg tttgcacatc ggtataccct catgcgcttg
     1801 atccggatgg actcaccgat cggaaatgcc agcaggtcga cgtccacatc gaggtcgctc
     1861 aaggcgccgg ccacataacg cacggcgatc ggtgtgccgc gatcctcgta gaacggttgc
     1921 ggcgtcacaa tcaggacccg gccccgattg accccatggc gatcgcgcag cagtggccgg
     1981 attttgcgat ttggactgaa gttcgcctgt ggcccggtcc aatcgactgg cggcagggcc
     2041 ggcaaattac gcgatatcac ggctcgcctc gagagtatgc acgggtatcg cgaagagagg
     2101 gcatgtaccg gtgccggtgc acgaacagcg gcggcaaaga tgatgtcata gagatgctcg
     2161 ctatgcgctc cggcgtgtag cggaagcttg tctcgctgac tctagggcac gttcctggtc
     2221 atgaccgttc ggtaccgaac ggaacaccgg gcgcgatctg gttaagttca ggtcttaact
     2281 ccatttgagc cttgaaccca cgatgcccac gcacatactt ctgattgccg acgacgccgc
     2341 cttcgcgact gccgcggtcg atgcgttgcc ggcaaccggc agcgtctgtt actccttcga
     2401 atgggttcgt cgctgcgccg acggggtcga tcggttggat cgtgatagca aggacacgag
     2461 ttcgcggctg gatccaattg ccgccgtcat ggtcgatctc gcgctgcccg atagtgtcgg
     2521 aatgacggcc ttcgaccagg tatttcacgc gacggggaac atccccattc tggttctcag
     2581 ccaagcgtcc gatgaggaaa tcgccaggca gaccctgcag catggggcgc aggactacct
     2641 gataaaggaa cgaatggaca gctattcgct gcgcaaggcg ctgagcggcg tcatcgaacg
     2701 cgcggcagcg tccagggaat tgaattcaga aaacgagcgc gcacaggtta ccctgaattc
     2761 gattggtgac gcggtgatca gcacggatgc ggtgggtcat gtgacttacc tcaattcagt
     2821 cgcggaacga ctcacggggt ggtcacaggc cgaagccgcc gaccggcaac ttg
//
LOCUS       DATMGX010000543         5043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_102828, whole genome shotgun sequence.
ACCESSION   DATMGX010000543 DATMGX010000000
VERSION     DATMGX010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102828"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VNR65_08790"
     CDS             <1..461
                     /locus_tag="VNR65_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18809.1"
                     /translation="LIGKRCAPRGSQRPGKRNPSSGVALAGGPARPYEMLRRSVKWYR
                     KISGDDWRKPGATLPFEGMSKANVRKLRDVRADAPEAANFRLKQISALFSWAVKEEHA
                     KVNPAEAWNAWRMRARASAPGPNRTFSPSSAIIRLERSRALRWHSFSGLA"
     gene            complement(973..1047)
                     /locus_tag="VNR65_08795"
     tRNA            complement(973..1047)
                     /locus_tag="VNR65_08795"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1012..1014),aa:Glu,seq:ctc)
     gene            1554..2096
                     /locus_tag="VNR65_08800"
     CDS             1554..2096
                     /locus_tag="VNR65_08800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWJ18810.1"
                     /translation="MKNDPRLQIVDFLPRLRRFSYALTGDSDKGDDLVQETCARALSR
                     INQWEPGTRLDSWMFRIAQNIWLDQRRAHKVRGDSVDLDQAYDIAGSDGREVAENRLT
                     LQEVSKGIAVLPGDQQVLIALVCVDGLSYKEAAEVLDIPIGTVMSRLARARRALCDFA
                     NPELETAPIKLETRRGQSNR"
     gene            2077..2922
                     /locus_tag="VNR65_08805"
     CDS             2077..2922
                     /locus_tag="VNR65_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18811.1"
                     /translation="MAKVTDEMLMAYADGALSPLARSKIEAFLQGDPEARRRVEIFRA
                     TGAPLSRIYGRPMAEPVPAYLKEFVLNYPINAETPKARSQKEGPSRWLEGLKNGTRHF
                     ATNLAEWLGSPAPAARWQLAAASTALLALGVTAGALVNGSSGVSSDLVAFNDGHIYAS
                     GPLGDVLEKDVSGRDTRIGGVRGQAVTMRASLTFKSKQNTYCREYEVATPSDGGFVGL
                     GCRGRDGKWALEVHLPADVSAKGGVKTAGGADNQALDAIVDRMSEGDVFGTEQEAAAI
                     RSGWK"
     gene            3010..3264
                     /locus_tag="VNR65_08810"
     CDS             3010..3264
                     /locus_tag="VNR65_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18812.1"
                     /translation="MVFRIKRGGADRRLACLLNADRLARCHGLELQNRIDEPLQHLGV
                     RSRCWAALSAVHFFVHMMWQRTRKPGPLLQKQNDANPFKL"
     gene            complement(3276..>5043)
                     /locus_tag="VNR65_08815"
     CDS             complement(3276..>5043)
                     /locus_tag="VNR65_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWJ18813.1"
                     /translation="GQQSGFGFFSVALAGEDDSKHHAGMDHADKDTGPHSDTAPHSNS
                     GVHGPAGQHNSGSVSGHTGGGDGKRRALFHGDGIPLGSHQASVSYDASRNMMKLHVPV
                     GGHLPLSGRRESHPVFNAGHSGSMGVSFGGHFADLQGKAATDTYIRNITGDKEKAGQT
                     ALALERSVTPVAARYHSSMFERSEGTTIMAKTSVPIGGLSFRPNEVLAVGANPASIDR
                     AQALGFKADPLMNSEAGDHTIVRFTVPPGLDAIRGQDLLSRELPGQRFELNRVYRLYR
                     ASAREEPVTLDKILPPTVPGNTPTCAANRCYAREVIGWKDPLAQCARALKVGVIDTDI
                     DEAHPAFDGRNIHRFDFAPDGHSAAPNWHGTAVLSLLAGSPVTSTPGLIPDAEFFAAN
                     VFYSDERGDMAADTVSLLRALDWMKKFDVKLINMSFSGPRDGLVGDAIARMSEMGVIF
                     VAAAGNEGPTAPPSYPAAYPQVIAVTAVTKELRNYRYANRGDHIDLSAPGVEIWTAAP
                     GSRAGYHTGTSFAAPYVTAMLAVQPPETLREGKADLLENLAVMDLGPEGRDPIYGRGL
                     LLAPSECTPSEETHDNIASAAE"
BASE COUNT         1061 a         1467 c         1527 g          988 t
ORIGIN      
        1 cgctcatagg taagcgatgc gcaccaagag gtagtcaaag accaggcaaa cgcaatccgt
       61 cgtcgggcgt cgcccttgca ggggggcccg ctcgcccata cgaaatgctg cggcggtcgg
      121 ttaagtggta ccgcaaaatc agcggtgatg attggcgaaa gcccggcgcc acacttccgt
      181 tcgagggcat gtcgaaggcg aacgtccgca agctgcgcga tgtgcgagcg gacgcgcccg
      241 aagccgcgaa tttccggctg aagcaaatct cggcgctttt ctcctgggcc gtgaaagagg
      301 agcacgccaa ggtcaacccg gccgaggcgt ggaacgcatg gcgaatgcga gcgagggctt
      361 ctgcacctgg accgaacagg acgttctcgc cttcgagcgc catcatcaga ttggaacgaa
      421 gccgcgcctt gcgatggcat tccttctctg gcttggcgtg agacgctcgg atgttatctt
      481 gctcggcccc tcccatgtcg ttgacgggct tgtctcgttt gtgcagttca agggtcgaaa
      541 gaagtcccag cggatcatca cgcttcccat cctgccaccc ttgcagcatg ccttgagaat
      601 acgcctttcg gcaaagagac ctatcttgag acagcttatg ggaagccttt cacagccccc
      661 ggcttcggga actggttccg gacgcaatgc gacgccgccg ggctgacgaa atgcagcgcc
      721 cacggcctcc cccgcaagat cggagcggtt cgagcggcag agaatggagc aagcgagcac
      781 gaactcatgg cgctgttcgg gtgggaaaac gccgagatgg cgccaaacta cacacgtaag
      841 gctgctcaga agaggatggc ggtcagcgcg gctgcgaaga tgtcgaaggg catgatcccg
      901 ggggacctgc gtttcccacc ttctgtccca cctgaagaaa atgcgttgaa tatcagagag
      961 ataaccaaga gatggcgccc cgtaggagaa tcgaactcct gtttcagccg tgagagggcc
     1021 gcgtcctgac cgctagacga acggggcagc tagaaaggag aggataaagt gagccgcgcc
     1081 tccaatcaag cagaaaggcg caatagactc cgagaaaccc caggagcggt aacgtacaca
     1141 atgcgtagcg atatagcgta cacaagaaac agcctttttc accacccgtg ggcgttacgc
     1201 cacactccgg cgcgaatctg agggttcgat cgcaaaatgc cactgcgcat cccgttgaca
     1261 cctgagaaca acggcttgac tcgcttacgc tgccatcgtg aaaaggtggc gataatatca
     1321 cccttatggt ggagcagcgt ctcctgccat gtgagaaggt gcatcagcca gtgcggcaga
     1381 gcgcaggaaa gcatgccacg agatagaatg cttcattcag ccgtttcagc gcccgggggg
     1441 cacacctgtt atttttttgc cacatgttgg aatggttcac cacactcttt cttccggtca
     1501 ggaattaagc agccacaccg gccgttattc gattgttccg gggaggcggt gacgtgaaaa
     1561 acgacccccg cctccagatt gtggactttc taccgcgcct gcgccggttc tcttacgctt
     1621 tgacaggaga ttccgacaag ggcgacgacc tcgtacaaga aacctgcgcg cgagcattat
     1681 cgcggattaa tcagtgggaa ccagggacaa ggctcgatag ctggatgttt cgtatcgccc
     1741 agaacatatg gctcgatcag cggcgcgctc acaaagtgcg tggcgattcc gtagacctcg
     1801 atcaggccta tgacatcgcc ggctctgatg gccgggaggt ggcggaaaac cggctgacgc
     1861 tccaagaggt atcgaaaggt atcgctgtgc tccctggcga tcagcaagtt ctgattgctc
     1921 tcgtttgcgt ggacggtctc tcctacaagg aagcggcgga ggtcctggac atcccgattg
     1981 gcaccgtgat gagccgcctc gcccgcgcgc ggcgtgctct ttgcgacttc gccaatccgg
     2041 aactcgaaac cgcgccgatt aagctggaga cccgccgtgg ccaaagtaac cgatgaaatg
     2101 ttgatggctt acgccgatgg cgcgctaagc cctctcgcgc gttccaaaat tgaagctttc
     2161 ctgcagggcg atcccgaagc ccgccggcgc gtcgaaatct ttcgtgccac cggagcgccc
     2221 ctttcgagga tttacgggcg gccgatggct gagccggttc cggcttatct caaagagttc
     2281 gttttgaact atccgatcaa tgcggagact cccaaggcgc ggtcacagaa agaaggcccc
     2341 tcaagatggc tggaagggct caagaacggg acgcgccatt ttgcaacgaa cctcgctgag
     2401 tggctgggaa gccccgctcc agccgcgcgc tggcagctcg cggctgcctc gacggcgttg
     2461 ctggccctcg gcgtgaccgc aggtgctctt gttaacggca gcagcggcgt ttcgagcgat
     2521 ctcgtcgcgt ttaacgacgg ccacatttat gcaagcgggc ccctcggtga tgtcctcgag
     2581 aaggacgtga gtggacgcga taccagaatc ggtggcgtgc ggggccaagc tgtcaccatg
     2641 cgcgccagcc tgaccttcaa gagcaagcaa aacacgtact gccgcgaata cgaggtcgcc
     2701 acccctagcg atggcggttt cgttggcctc ggatgcaggg gccgggacgg gaaatgggct
     2761 cttgaagttc accttccggc agatgtttct gccaagggcg gggtgaagac cgctggtggt
     2821 gccgataatc aagcgctcga cgcgatcgtg gatcgcatga gcgagggcga tgtatttggc
     2881 actgagcaag aagcagctgc cattcgaagc ggctggaaat agctcggaag ccgacgagac
     2941 aagattgcct tagctgaaac tgatcaaggc cgggcttgac ccggccattg gtttaggcgg
     3001 agttgatgga tggtgttccg catcaagcgc ggaggcgcgg accgccgtct cgcctgcctt
     3061 ctcaatgccg accgccttgc ccgttgccat gggctcgaac ttcaaaaccg catcgacgaa
     3121 ccgctgcaac accttggtgt caggtcacgg tgttgggcgg cgctgagcgc ggtccatttc
     3181 ttcgtgcaca tgatgtggca aaggaccaga aagcctggac ctcttctgca aaagcagaat
     3241 gatgcaaatc cgttcaaact ttagctgata gcgctctatt ctgccgcgct agcgatgttg
     3301 tcatgagttt cttctgacgg cgtgcactcc gatggcgcta gaagcagtcc tcgaccgtaa
     3361 atcgggtcac gaccttctgg gccaagatcc ataacggcga ggttctcaag aagatcggcc
     3421 ttcccctccc ggagagtctc gggaggctgt acggcaagca tagccgtgac gtatggtgct
     3481 gcgaacgagg tgcccgtgtg gtatcccgca cgcgaacctg gagccgctgt ccagatttca
     3541 acgccagggg cggagagatc gatgtgatct cccctgttgg catagcggta attgcgcaat
     3601 tctttcgtga ccgctgtcac ggcgatcact tggggataag cggccgggta gctcggcggc
     3661 gccgttgggc cttcgtttcc ggccgcagcg acgaagatga cgcccatttc gctcatccgt
     3721 gcgatggcgt cgccaaccag gccatcccgc ggaccagaaa aactcatatt gatgagcttc
     3781 acgtcgaatt tcttcatcca atcgagcgcc ctcaagaggc tgacggtatc ggccgccata
     3841 tccccgcgtt catcggaata gaagacattg gcggcgaaaa attccgcgtc cgggataaga
     3901 cccggggtgc tcgtaacagg acttccggcc agcaacgaga gaactgcagt tccgtgccaa
     3961 ttcggggcgg ccgaatgccc atcgggagcg aaatcgaacc ggtgaatatt gcggccgtcg
     4021 aaagcagggt gcgcttcgtc gatgtcggtg tcgatcacgc cgaccttcag cgccctggca
     4081 cattgcgcga gcgggtcttt ccagccgatc acctcccgcg cgtaacaacg gtttgcggcg
     4141 catgtcggag tattgccggg aaccgtcggc ggcaggatct tgtcgagcgt caccggctcc
     4201 tcacgagctg acgcacgata gagacggtag actctattga gctcgaaccg ctgtcccggc
     4261 agctctcggc tgagaagatc ctggccgcga attgcgtcca gtccgggagg cacggtaaag
     4321 cggacgatcg tgtgatcgcc cgcctctgaa ttcatgagcg gatcagcctt gaacccaagg
     4381 gcttgggcac gatcgatgga ggccgggttc gctccgacgg caagcacctc gttcggccgg
     4441 aacgagagcc caccgatcgg aacactcgtc ttggccatga tcgtcgtccc ctcgcttcgc
     4501 tcaaacatcg agctgtgata gcgggcggca acaggtgtga cggacctttc cagagccagc
     4561 gctgtctggc ctgccttttc cttgtccccg gtgatattgc gaatataggt gtcggttgca
     4621 gcttttccct ggagatccgc aaaatgtccg ccgaagctca cgcccatact tccggagtgg
     4681 cccgcgttga acacgggatg cgactcgcga cgtccgctta gtggtagatg cccaccaact
     4741 gggacatgca gcttcatcat attgcggctt gcatcataac tgacggacgc ctggtgagat
     4801 cctagcggga tgccatcgcc atgaaacagt gcacgacgct tcccatcccc gcctccagtg
     4861 tgaccggaga cactaccgct attgtgctgt ccagcgggac cgtgaacgcc ggaattgctg
     4921 tgaggagcgg tatcgctgtg agggccggtg tccttgtcgg cgtggtccat cccggcgtga
     4981 tgttttgaat catcctcgcc tgccaaagcg acagagaaaa acccgaagcc ggattgctgg
     5041 cct
//
LOCUS       DATMGX010000544         3021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_202674, whole genome shotgun sequence.
ACCESSION   DATMGX010000544 DATMGX010000000
VERSION     DATMGX010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202674"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..553)
                     /locus_tag="VNR65_08820"
     CDS             complement(38..553)
                     /locus_tag="VNR65_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWJ18814.1"
                     /translation="MASKSPAGYSGTQILLHWIIAALILFQLLFSEGMEHAYNAWVKG
                     KEIGADNLLSANIHTVVGFTVLALAVLRVIFRMVRGVPDAPAGQSGVKLWIAALTQVF
                     LYAILFVMPITGAMAWFLGYASMAEVHAFAKPLIILAVLLHFGGALMQHYVAKTDVLV
                     RMMKPEAKRVA"
     gene            701..2326
                     /locus_tag="VNR65_08825"
     CDS             701..2326
                     /locus_tag="VNR65_08825"
                     /inference="COORDINATES: protein motif:HMM:NF006020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWJ18815.1"
                     /translation="MTSAYTQDLDRTPANFQQLTPLGFLARAASVFPNYPAIVHGDRT
                     TSYAEFYARSRRLGSALMRLGIGKSDTVSVMLANTPAMLEAKFGVPMTGAVLHSINTR
                     LDAANIAFMLDHAETKVLITDTEYSKVIGEALTIAKARPIIIDYMDPEFPGLGSRLSE
                     TEFEELLAGGDPEFAWKMPDDEWDAITLNYTSGTTGNPKGVVYHHRGAYLLSLGNTLA
                     ASMGKHPQYLWTLPMFHCDGWCFPWTIPVVAGTQVCLRYVRPDAIWNALADHKITHLC
                     GAPIVMSTILSAPNDVKRKLSQKVEFLTAAAPPPEAVLAEMREAGFNVTHVYGLTEVY
                     GPAVVNEWKEEWNALDSAGQAQKKARQGVRYVPLEDLTVLDPGTMMPVPHDGETLGEV
                     MFRGNVVMKGYLKNKQATEDALAGGWFHSGDLGVMYPDGYIQLKDRSKDIIISGGENI
                     SSIELEDVLYKHPAVQAASVVAKYDEKWGETPCAFIELKSGKTATAEEIIAYCRQHVA
                     HYKVPRHVVFEELPKTSTGKVQKFVLRQMAKKV"
     gene            2565..>3021
                     /locus_tag="VNR65_08830"
     CDS             2565..>3021
                     /locus_tag="VNR65_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18816.1"
                     /translation="MRLVFKAVISAAILYCGFAVPAAADEPAKPSLWSFGVTAGTLGA
                     GVEASARVYDNVVFRVNGSYVKLDSSWVLSASSLAKDYNLDVAGIFAGGILDYHPFSS
                     GWRLSAGARYVDIDLQGVAKNGMSLGGVSYTADQVGNVTATVRNGNPVAP"
BASE COUNT          644 a          861 c          880 g          636 t
ORIGIN      
        1 ttcccaaaac cgggaaggcc acttttggga actctgccta ggcgacgcgt ttcgcctcgg
       61 gcttcatcat cctcacgagc acgtcggtct tcgcgacgta atgttgcatc aatgccccgc
      121 cgaaatgcag aagcacggcg aggataatca gtggctttgc gaaggcatgc acctcagcca
      181 ttgaggcata acccaagaac catgccatcg cgccagtaat cggcatcacg aaaaggatag
      241 catagagaaa gacttgcgta agcgctgcga tccatagctt gacgccgctc tggccagcag
      301 gcgcatccgg aacgccgcgg accatgcgaa atataacgcg caagactgcc agcgcgagga
      361 ccgtgaagcc cacaacggtg tgaatgttcg cgctgagcaa attgtctgcg cctatctctt
      421 tgcctttcac ccaggcgtta taagcgtgct ccatgccctc actgaagagg agctggaaaa
      481 gaatgagcgc ggcgataatc cagtgaagaa ggatttgtgt gccgctgtag ccagccgggc
      541 tcttggacgc cattgcattc tccaaaaatt gcaaatttta agtcactgca tacaccggct
      601 tttccggcag aaatttgatc tgtgacaaac gcagcctggg cagaagcacg cagcctgctg
      661 taagcttagc ccggcccacc tcaaaccagc gacgcgtgtc atgacaagcg cctatactca
      721 agatctcgac aggacgcccg ccaattttca gcaattaacg ccgctcggct ttcttgcccg
      781 tgccgcgagc gtcttcccca actatcccgc catcgtgcat ggcgaccgca caacctcata
      841 tgccgagttc tatgcgcgat cccgccgcct cggatcggcg ctaatgcgcc tcgggatcgg
      901 caagagcgac accgtctcag tcatgctcgc gaatacgcct gcgatgctgg aggccaagtt
      961 cggtgtgccg atgacgggtg cagtcctgca ttcgatcaat acgcggctcg acgcggccaa
     1021 tatcgccttc atgctcgacc atgccgaaac gaaggtgctc atcaccgaca ccgaatattc
     1081 gaaggtgatc ggggaagcgc ttacgatcgc gaaggcgcgg cccatcatca tcgactatat
     1141 ggacccggag tttcccggcc tcggctcgcg gttgtccgag acggaattcg aagaacttct
     1201 cgcgggcggc gacccggagt tcgcctggaa gatgccggac gacgagtggg acgcgattac
     1261 cctcaactac acctcgggca cgacgggtaa cccgaagggc gtcgtctatc accatcgcgg
     1321 ggcctatctc ctgagccttg gaaatacgct cgccgccagc atgggcaagc atccgcagta
     1381 tctctggacg ctgccgatgt tccattgcga tggctggtgc tttccttgga cgatcccggt
     1441 cgtcgccggg acgcaagtct gccttcgtta tgtccgccct gacgcgattt ggaacgcgct
     1501 cgccgatcac aaaatcaccc atctctgcgg cgcgcccatc gtcatgtcga cgatcctgag
     1561 cgcccccaat gatgtaaagc ggaagctctc tcaaaaggtg gaatttctga cggcggccgc
     1621 cccgccgccc gaagcggtgc tcgcggagat gcgcgaagcg ggcttcaatg tcacccatgt
     1681 ctatgggttg accgaggtct acggccccgc cgttgtcaat gagtggaagg aagagtggaa
     1741 cgcgctcgac agcgccgggc aggcgcagaa gaaggcgcgg cagggcgtgc gctatgtccc
     1801 tcttgaagac ctgaccgtgc tcgaccctgg cacgatgatg cccgtcccgc acgatggcga
     1861 aacgcttggc gaggtgatgt tccgcggcaa tgtcgtcatg aaggggtatc tcaaaaataa
     1921 gcaggccacc gaggatgcgc tggcaggcgg ctggttccat tccggcgacc tcggcgtgat
     1981 gtatccggac ggctacatcc agctcaagga ccgctcgaag gacatcatca tctcgggggg
     2041 cgagaacatc tcgtccatcg agttggagga cgtgctgtat aagcaccccg ccgtgcaggc
     2101 tgcttcagta gtggcaaagt acgacgagaa gtggggcgag acgccttgcg ccttcatcga
     2161 gctgaagtcg gggaagactg cgacggccga agagatcatc gcttactgcc gccagcatgt
     2221 ggctcattac aaagtcccgc gccacgttgt cttcgaggag ttgccgaaga cgtcgacggg
     2281 caaggtgcag aagttcgtcc tgcgccagat ggcgaagaag gtttagccct agcgggaggc
     2341 cgggggtgag ctgttgcatt tgcacaggtc atccccgaat ttttgggcgc gtcgctgaac
     2401 taataactag ctaactttat ccaatatgat aaaagtgctt taatattttg ggcttggttt
     2461 ttgggcggga tgcccagttg ccggcgcctg aaaatgctag gcttacgtaa gtttgagccg
     2521 ccgtctgaga caaaagagtt ttttgatttt tgtcggggat attcatgcgt cttgttttta
     2581 aggctgtgat cagcgcagca attctttatt gcggattcgc cgttcctgcc gcggctgacg
     2641 agcccgccaa gcctagttta tggtcgttcg gagtgacagc tggaacgctt ggagccggcg
     2701 tcgaggcctc tgcccgcgta tacgacaacg ttgtttttcg cgttaacggc tcgtatgtga
     2761 agcttgactc cagctgggtg ctctctgcct ccagtttggc gaaagattat aaccttgatg
     2821 tcgcgggcat atttgctggc ggcattctgg attaccatcc attcagcagc ggatggcgct
     2881 tatcggcagg tgcccgctat gtcgacattg atctgcaagg cgtggcaaag aatggaatga
     2941 gtctcggagg ggtctcgtac acagctgatc aagttgggaa tgtcaccgcg acggtacgca
     3001 atggcaaccc cgtggcccct t
//
LOCUS       DATMGX010000545         2687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_53588, whole genome shotgun sequence.
ACCESSION   DATMGX010000545 DATMGX010000000
VERSION     DATMGX010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53588"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VNR65_08835"
     CDS             <1..689
                     /locus_tag="VNR65_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWJ18817.1"
                     /translation="LKAWRAARDANAVQASLAALRAAAQEGRNIMEPSIACAKVGVTT
                     GEWGDTLRQIFGEYRAPTGVSRAAAQGGGAGIAEVRADVDRVSAKLGRRVKFLVGKPG
                     LDGHSNGAEQIAVRARDSGMEVVYEGIRLTPAQIVNAALEESVHVVGLSILSGSHVPL
                     VRDIMDRMRAAGLGAIPVVVGGIIPVEDEATLKACGVAAIYTPKNFQLNDIMAGIVKL
                     IDPDLRALAS"
     gene            715..1332
                     /locus_tag="VNR65_08840"
     CDS             715..1332
                     /locus_tag="VNR65_08840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ18818.1"
                     /translation="MIQPGHNGEVRRLEISTLTGQELQKALPQLAALRMEVFRDYPYL
                     YDGSLDYEESYLTALIESKDSIIVAAEDGGKIVGCATGSALQGHHQEFAAPFLEHGFD
                     PNEVFYCGESVLLPSHRGRGLGHTFFERREAHAKARHYKYSAFCGVVRPDNHPLRPAT
                     YSPLDTFWKKRGYRKAEGMVATFRWKDIDQPQETDHPMQFWIREL"
     gene            1332..2234
                     /locus_tag="VNR65_08845"
     CDS             1332..2234
                     /locus_tag="VNR65_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWJ18819.1"
                     /translation="MPRVIRVAAAQYAFDEITNFAAFEDKLSQWVQQAVAHGAQLLVF
                     PEYAAMELARIAGRQISLDLHGSIEALQYHIGHYEAAYLELAKRHGVFILAGSAPTRL
                     TDGRFVNRAQFFSPNGHSGFQQKHIMTRFEAEQWGISASAGLCIFDIGVAKIGIAICY
                     DSEFPLIARALAEGGADILIVPSCTESLAGYYRVKHACAARALENQMYVIQSPTVGEA
                     PWTVGVDINVGAAGFFAPPDKRFPADGVLVQGTLNKAEWVYADLDLDLIAEVRNDGDV
                     LNARDWPLQPGAPVLPRPKIVTLA"
     gene            2398..>2687
                     /locus_tag="VNR65_08850"
     CDS             2398..>2687
                     /locus_tag="VNR65_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18820.1"
                     /translation="MFSIIFGPAMAIMSRLRFALKLGLVGILFLVPLSGLVYYLYGTL
                     HSDIQLAENEREGVQQILPGRYFVQTVQAHRGASQIAISGDAAAKQKLADFT"
BASE COUNT          540 a          803 c          801 g          543 t
ORIGIN      
        1 cgctgaaagc gtggcgcgcc gcccgcgacg ccaatgcggt gcaggcttct ttagccgctc
       61 ttcgagccgc cgcacaggaa ggccgcaata tcatggaacc gtccatcgcc tgcgccaagg
      121 ttggcgttac gaccggggaa tggggtgaca ccttgcgcca gatcttcggc gagtaccgcg
      181 cgccaaccgg cgtctcccgc gctgcggccc agggcggcgg agcaggcatt gccgaagtgc
      241 gcgccgatgt cgatcgcgtc tctgcaaagc ttgggcggcg ggtgaagttc ctcgtcggca
      301 agcccggcct cgacggacat tccaatggcg cggagcagat tgccgtgcgt gcgcgggact
      361 ccggcatgga ggtggtctac gaaggcatcc ggctgacgcc cgcgcagatc gtgaatgcgg
      421 cgcttgagga aagcgtgcac gttgtcggcc tctcgatcct ttccggcagc cacgtgccac
      481 tcgttcgcga catcatggac cgtatgcgcg cggcgggtct tggcgcgatc ccggtcgtgg
      541 tcggcggcat catcccggtg gaggacgaag caaccttgaa ggcttgcgga gtcgctgcga
      601 tctacacacc gaagaatttt caattgaacg acatcatggc gggaattgtg aagctcattg
      661 acccggatct tcgggctctc gcttcgtaaa cgcttgcctt tgtcgagcgc gcgggtgatc
      721 cagcccggcc acaatggaga ggttcgacgg ttggagattt cgacgctaac cggccaggag
      781 ctgcagaaag cgttgcctca attggcggcg ctcaggatgg aagtctttcg cgactacccg
      841 tatctctacg atggttctct cgactatgaa gagagctatc tgacagccct aatcgaaagc
      901 aaagacagca ttatcgtggc tgccgaggac ggcgggaaaa tcgtcggctg cgccaccgga
      961 tcggccctcc aagggcatca ccaagagttc gccgcgcctt tcctggagca tggcttcgat
     1021 cccaatgagg tgttctattg cggagaatcg gtgctgctgc cgagccaccg gggacgaggc
     1081 cttggccaca cattttttga acggcgagaa gctcacgcca aggcacgcca ctacaagtac
     1141 agtgcgtttt gcggggtcgt gagaccggac aatcacccgc tcagacccgc aacctattcc
     1201 ccgctcgata cgttctggaa gaaacgcggg taccggaaag ccgaagggat ggtcgccaca
     1261 ttccgctgga aagacatcga tcaaccgcag gagaccgatc atccgatgca attctggatc
     1321 agagaactgt aatgccgcgt gtcatccgcg tcgcagcagc gcaatatgcc tttgacgaaa
     1381 taaccaactt cgccgccttc gaggacaagt tgtcgcagtg ggttcagcag gcggtcgcgc
     1441 atggcgccca actcctggtg ttcccggagt acgctgcgat ggaactcgcc cggatcgcag
     1501 ggcggcagat atcgctcgac ctgcacggct ccatcgaagc gttgcaatac catatcggcc
     1561 actacgaggc ggcttatttg gagcttgcga agcgccacgg cgttttcatt ctcgcgggta
     1621 gcgccccgac gcgcctgacg gacggccgtt tcgtgaaccg cgcccagttc ttcagcccga
     1681 atggacattc tggcttccag cagaagcaca tcatgacgcg tttcgaggcc gagcaatggg
     1741 gtatcagcgc cagcgccggc ctttgcatct tcgatatcgg ggtcgcgaaa atcggcatcg
     1801 caatctgtta cgattccgag tttcccctga tcgcgcgcgc tctcgccgag ggaggcgccg
     1861 acattctgat cgtgccgtcc tgcactgaaa gtctggcggg atactatcgg gtcaaacatg
     1921 cttgtgccgc cagggcgctt gaaaaccaga tgtacgtcat tcaatcaccc actgtggggg
     1981 aagcgccgtg gaccgttggc gtcgatatca atgtcggcgc tgccgggttt tttgcaccac
     2041 ctgacaagcg atttcccgct gacggcgtcc tcgttcaggg cacgctcaat aaagcggaat
     2101 gggtctatgc ggatctcgat ctcgacctca tcgccgaggt gcgaaatgat ggagacgtgt
     2161 tgaatgcccg cgattggccg ttgcagcccg gagcgccggt ccttccccgc ccaaaaatcg
     2221 tgacgctcgc ctaaaccggg tcaagtgtcc caaaaggaga gcgagcaggc ctcggcatcg
     2281 ccgtatgcgc tgcaacgtag gtgtatgaaa ctgcctgaat tttagacttc cgctcaacct
     2341 taatccaacc ctcatagtgc ataactcaag ctgctatttc tccttgaggc gctaaaaatg
     2401 ttttccatca tcttcggtcc ggccatggcg atcatgagcc gccttcgctt tgcgcttaag
     2461 ctcgggctgg tgggaatcct gttcctcgtg cctctttcgg ggctcgtcta ctacctctat
     2521 ggaacactcc actccgacat tcaattagcg gagaacgagc gtgagggcgt tcaacagatt
     2581 ctgccgggcc gctatttcgt gcaaacggtc caagctcacc ggggcgcaag ccagattgca
     2641 atcagcggcg acgcggcggc caaacagaag ctcgcggact ttacaag
//
LOCUS       DATMGX010000546         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_27543, whole genome shotgun sequence.
ACCESSION   DATMGX010000546 DATMGX010000000
VERSION     DATMGX010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27543"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1277)
                     /locus_tag="VNR65_08855"
     CDS             complement(192..1277)
                     /locus_tag="VNR65_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004867878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWJ18821.1"
                     /translation="MRGKFADQGGLFSYIAPDKRVPANHPLRKIRELVRDVLGELNRS
                     LGKLYASEGRPSIPPEQLLSALLLQVFYGIRSERQLMEQLDYNLLYRWFVGLSPDDAV
                     WDPTTFTKNRDRLQNGDVFMKFMTRLLNHSQVKPLLSDEHFSVDGTLIEAWASQKSFR
                     PKDGSGDDDDGANFHGQKRKNDTHASTSDPDSRLYRKAAGREAKLCYMGHATMENRHG
                     LAVAGLVTHANGTAERRASEIMLKARSKAAGHRITAGEDKAYDTADHVANLRAINVTP
                     HVTQNNGLTKTGRTRKSAIDERTTRHPGYGMSQSRRAMAECIFGWGKQHGTMRKTKHR
                     GIARVAANFMLNLIAYNLICIPKLMAA"
     gene            complement(1457..>2034)
                     /locus_tag="VNR65_08860"
     CDS             complement(1457..>2034)
                     /locus_tag="VNR65_08860"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWJ18822.1"
                     /translation="GRGYTIFDTGIGRCGIAWSHAGIVGVQLPEAREIETRKRLFKLY
                     PDAREQRPPGDVELAIEGIVALLRGESCDLSEVPLDMNGILAFHARVYAFTRGIPRGE
                     TITYGEIAAKLRLSGAVHSVAQALARNPFVIIVPCHRVLEAGGYADKISLHGGAISKR
                     RLLSIEGARGPTASKTLFDVLLPVAPPRPQG"
BASE COUNT          382 a          601 c          629 g          422 t
ORIGIN      
        1 ccaagacgac catttctgat tttccgaatt ttacttgacc ccgaccccaa atcagcacta
       61 tcagtttgct gaaaaacctg gaaatcatgg ttttctttgt cttggcggtg ccgtcgatca
      121 agtttcaggt gcagtgttta ccggcttgag gcagttttgt ttgcctgtgc gggtgctgcc
      181 acccggcact gctacgcagc catcagtttg ggaatgcaga tcaggttgta ggcgatcaga
      241 ttgagcatga agttggcggc gacgcgagcg atgccgcgat gcttggtttt gcgcatggtg
      301 ccgtgctgct tgccccaacc gaagatgcac tcggccatcg cccggcgtga ttgcgacatg
      361 ccatagcctg gatgtcgcgt ggtgcgctcg tcgatggcgc tcttgcgggt cctgccggtt
      421 ttggtgaggc cattgttttg tgtcacatgc ggcgtcacgt tgatggcgcg aaggttagcc
      481 acgtggtcgg cggtatcgta ggccttgtcc tcgccggccg tgatgcggtg gccggcggct
      541 ttgcttctgg ccttcagcat gatctccgag gcccggcgtt cggcggtacc attggcgtgc
      601 gtgaccaagc cggccaccgc cagcccatgg cggttctcca tggtggcgtg gcccatatag
      661 caaagcttcg cctcccgccc ggcggccttg cggtaaagcc tgctgtccgg gtcgctggta
      721 ctcgcatggg tgtcgttctt gcgcttctgg ccatggaagt tcgcgccgtc atcatcgtca
      781 ccgctgccgt ccttggggcg aaaactcttc tgtgaagccc aggcttcgat cagcgttccg
      841 tctaccgaaa aatgctcgtc cgacaacagt ggcttgacct gagaatggtt gagaagcctg
      901 gtcatgaact tcatgaacac atcgccgttc tgcagccggt cgcggttctt ggtgaaggtg
      961 gtcgggtccc agaccgcatc atccggcgac agtcccacaa accagcgata caaaagattg
     1021 tagtccaact gctccatcaa ctggcgttcc gagcggatgc cgtagaacac ctgcagcaac
     1081 aaggcgctca gcaattgctc cggggggatc gaggggcgtc cctcgctggc atagagcttc
     1141 ccaaggctac ggttcaactc acccaaaaca tcccggacaa gctcccggat cttccgcagc
     1201 ggatggttag ctggcacacg cttatccggt gcgatgtacg agaacaggcc gccctgatcc
     1261 gcaaacttgc cacgcatgat cgtctccggc gattcgagat gataaagtga atcatcaagc
     1321 cccatccgtg gcgagggggt ttttcagcag actgctagag cgttttcgag cgaagtggaa
     1381 accggttcgc gttaagaaaa cgcgtcaaat caggaatcca gagccccgtt tcgattcaat
     1441 cgaaacggaa aaggctctag ccctgcgggc gcggcggagc aacggggagc agcacgtcga
     1501 acagcgtctt gctggcggtc gggccgcgcg cgccttcgat cgaaagcagc cggcgtttgg
     1561 aaatcgcccc gccatgcagc gagatcttgt cggcatagcc gccggcttcc agcacgcgat
     1621 ggcagggcac gatgatcacg aaggggttac gggccagcgc ctgcgccacc gaatgcaccg
     1681 cgccggaaag ccggagtttg gcggcgatct cgccataggt gatggtttcg ccgcgcggaa
     1741 tgccgcgggt gaaggcgtag acccgggcgt ggaaggcaag gatcccgttc atgtcgagcg
     1801 ggacctcgga aagatcgcag gattcgcccc gcagcaatgc gacgatgccc tcgatcgcca
     1861 gttcgacatc gcccggcgga cgctgctcgc gggcgtcggg atagagcttg aacagccgct
     1921 tgcgggtttc gatctcgcgg gcctcgggca actgcacgcc gacgattccg gcatggctcc
     1981 aggcgatgcc gcaccggcct atacccgtat cgaaaatggt gtacccacgc cccg
//
LOCUS       DATMGX010000547         2689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_3288,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000547 DATMGX010000000
VERSION     DATMGX010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3288"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..2076)
                     /locus_tag="VNR65_08865"
     CDS             complement(64..2076)
                     /locus_tag="VNR65_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18823.1"
                     /translation="MEPFKALVLLAAAAVVSFVASGAVGFYAGYSAGRGNLDEIKSHI
                     SSSQVVIPSGNGGTVSLKPVLDDVRALAKQVEALAAVKANAAADNDSGTKLVLDEIRG
                     LSSQVDGLRQDTLKPLPTPSPAIERPISTPPVHDYTDALQEIRDQVRSLNAKVEKQEP
                     KAPKALMDDIRALSASIQAQEPALKRALGEEVRSAVASLQSGEQKTPKALVEELKVLN
                     SSIQSQEPNVRRVVAEEVRSAVASLQSGEQKSPKALVEELKVLNSTIQAQEPNVRRVV
                     AEEVRAAVASLQSGEQKSPKALVEELKSLSSSIQSQEPNVRRAVAEEVRSAVASLQSG
                     EQKSPKALVEELKVLNSSIQSQEPNVRRAVAEEVRGAVASLQSGEQKSPKALVEELKV
                     LSSSIQLQEPNVRRAVAEEIRTAVASLQSGEQKSPKALVEELKVLSSSIQSQEPNVRR
                     AIAEEVRTAVATLLNAEPKVPKAILDEIRAISANAKAQESRPQTTQSSVVDQIKVLQA
                     SIELLRAKLLEERGRTDSAASADIAQLRQLVTTASDHFSRCQTQLASLPGPGSGAVAQ
                     QAAAPTAAAITISQEKKTEPAAVVFYDNVMLKKDQEKQYDEIGVRLSLQSVGSRQVRV
                     AVNRQGFGLAFGERKVFRSQDVECEINLMETNLNEGQARVSISCKR"
     gene            complement(2144..>2689)
                     /locus_tag="VNR65_08870"
     CDS             complement(2144..>2689)
                     /locus_tag="VNR65_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ18824.1"
                     /translation="AIAFSDKDQLDGRYLETTADNFSKTMLISCYSLTAIAQRAEKLL
                     TDGGSILTLTYYGAEKWMPHYNVMGVAKAALEASVRYLAADLGEKNIRVNAISAGPIK
                     TLAASGIGDFRYILKWNEYNSPLRRTVSIEDVGGAGLYLLSDLSRGVTGEVHHVDAGY
                     HVVGMKAEDAPDIAVVQPSNR"
BASE COUNT          527 a          805 c          770 g          587 t
ORIGIN      
        1 tggaggcatt ataatcggtc tgaccgtgcc tcacgaaata aatgagcggc attgtggttg
       61 ccctcagcgc ttgcaggaga tgctgacgcg ggcctgcccc tcgttgagat tagtctccat
      121 cagattgatc tcgcactcaa catcctggct gcggaacacc ttgcgctcgc caaacgccaa
      181 gccgaaaccc tggcggttga ccgcaacgcg aacctgccgg gaccctacgg attgaagcga
      241 gagccgcaca ccgatctcgt catattgctt ttcctggtcc ttcttcagca tgacattgtc
      301 gtaaaagacc acagcggcgg gctctgtttt cttctcttgc gatatggtga ttgcggcggc
      361 tgtgggagcg gcagcttgct gcgcgacagc tcccgaccct ggtccaggaa gcgaagcaag
      421 ctgcgtctgg catctgctga agtggtccga tgcggttgtc acaagctgac gaagctgagc
      481 gatatcagcg cttgccgcgc tgtcggtgcg gccacgctct tccaggagct tcgccctcag
      541 gagttcgatg gaagcctgga gaactttgat ctgatcgaca accgaggatt gggtggtctg
      601 cggacgtgac tcctgcgcct tggcgttagc cgaaatggcg cgaatttcgt ccaggatcgc
      661 cttcggaacc ttcggctccg cgttcagcaa agtcgcgaca gccgtgcgca cttcctcggc
      721 tatggctcgc cgcacattcg gctcctgcga ctggatggag gaactgagca cctttaattc
      781 ctcgacgaga gctttcggcg atttctgctc tccactttgc aaggatgcaa cggcagtgcg
      841 aatttcctcg gcgaccgcgc gccgcacgtt cggctcctgc aactggatgg aggaactcag
      901 caccttcaat tcctcgacga gagctttcgg cgatttctgc tctccacttt gcaaagatgc
      961 gacggcaccg cgaacttcct cggcgaccgc gcgccgcaca ttcggttcct gcgactggat
     1021 ggaggaattg agcaccttca attcctcgac gagagccttc ggtgatttct gctctccgct
     1081 ttgcaacgag gcgacggcag aacgaacttc ctcagccacc gcgcggcgca cattcggctc
     1141 ctgcgactgg atggaagaac tgagcgactt taattcctcg acgagagctt taggcgattt
     1201 ctgttctccg ctttgcaaag atgcgacggc agcacgaact tcctcggcga ccacgcgccg
     1261 tacatttggc tcctgagcct gaatggtgga attgagcacc ttcaattcct cgacgagagc
     1321 cttcggcgat ttctgctctc cgctttgcaa cgatgcaacg gcagagcgaa cttcctcggc
     1381 gaccacgcgc cgcacattag gctcctgcga ctggatggag gaattgagca ccttcaattc
     1441 ctcgacgaga gctttcggcg ttttctgctc tccgctttgc aacgaggcga cggcagagcg
     1501 aacttcctcg ccaagcgcgc gcttcaaagc cggctcctgt gcctgaatgg aagcgctgag
     1561 agcacgaatg tcatccataa gagcttttgg cgctttcggc tcctgcttct ccactttggc
     1621 gttcagggag cggacctgat cgcggatttc ctgcagcgcg tccgtgtaat cgtgcacagg
     1681 cggcgtagaa atcggccgct ctatggcggg ggacggcgtc ggcaaaggct tcaaggtatc
     1741 ctgcctcaag ccatcgactt ggcttgagag gcccctgatc tcatccaaaa ccagcttcgt
     1801 gccggagtcg ttgtcggcgg cggcattcgc cttgactgcg gcaagcgctt ccacctgctt
     1861 cgcaagggcg cgaacatcgt caaggacagg cttcagcgaa acagtgccgc cattgccgga
     1921 tggaataaca acctgactgc ttgagatgtg cgatttgatc tcgtcgaggt tgccccgacc
     1981 tgcggagtag cctgcataga aacccaccgc tcccgaggct acgaaagaca caaccgccgc
     2041 tgcggcaagc aaaaccagcg ccttaaatgg ttccatgaat gagcccgccc caatgccgcg
     2101 cgaatcggca ttctataaat gatcgccttt agttcttatg atttcagcga ttcgatggct
     2161 gaacgacggc gatatccggg gcatcctcag ctttcatgcc cacaacatga tagccggcgt
     2221 caacgtgatg cacctctccg gtaacgccgc ggctgagatc ggagagaaga taaaggcccg
     2281 cgccgccgac gtcttcgatt gagaccgttc tgcggagcgg cgaattgtat tcgttccact
     2341 tcaggatata gcggaaatcg ccgatgccgg acgcggccag cgtcttgatc ggtcccgccg
     2401 agatcgcgtt gacgcggatg ttcttctcgc cgaggtcggc ggcgagatag cgcacgctgg
     2461 cctcgagcgc tgccttcgcg acccccatca cgttgtaatg cggcatccat ttctcggcgc
     2521 cgtaataggt cagcgtcagg atcgagccgc cgtcggtcag aagtttctcg gcgcgctgcg
     2581 cgatcgccgt cagcgaatag cagctgatca gcatggtctt ggaaaaatta tccgccgtgg
     2641 tttcgagata gcggccgtcg agttggtcct tgtcggagaa cgcgatggc
//
LOCUS       DATMGX010000548         5705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_35115, whole genome shotgun sequence.
ACCESSION   DATMGX010000548 DATMGX010000000
VERSION     DATMGX010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35115"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..749
                     /locus_tag="VNR65_08875"
     CDS             138..749
                     /locus_tag="VNR65_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ18825.1"
                     /translation="MIYEGLDFMIRRKERWCVMGINGAGKSTLLKLVAGVSKPDQGSV
                     TIGGSVKMGYFAQHAMELLDGDRTIFQSLEDEFPQAGQGTLRTLAGCFGFSGDDVEKK
                     CRVLSGGEKARLAMALMLFDPPNFLVLDEPTNHLDMATKEMLITALAQYEGTMLFVSH
                     DRHFLSALSNRVLELTPEGVHSYNGGYAEYVARTGHEAPGLRG"
     gene            complement(1126..3879)
                     /locus_tag="VNR65_08880"
     CDS             complement(1126..3879)
                     /locus_tag="VNR65_08880"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF003467.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HWJ18826.1"
                     /translation="MDQVNFATPPYFDPAALREEFDSYWREGSHTPSKTRARVVGRLK
                     ELKYNARTEAQALLMRTGDGCACARSLAQFQDELIKLIYDYSTKHLYRAQNPSAAEHM
                     AIVATGGYGRGMMAPSSDVDLLFLLPYKQTAWGESIVESILYFLWDLGYKVGHATRTV
                     DQTLKAAREDMTIRTALLDARLLYGEGALFRELWTRFIASVVQGSQRDFIEAKLAERE
                     ERLRRAGVSRYRVEPNIKEGKGGLRDLNMLHWLSAYLNPGKNGQAENKVFAPQEVSTF
                     HHCEAFLWTIRCHLHFLTGKPDERLSFDLQPEMASRLRYHDRKGLLGVERFMKHYFLV
                     AKDVGDLTRTICSSLELRQLKTVPTLSDFYGVLPWSSRAKLAATTDFRFDNGRLSVKQ
                     PDAFRKDPLNLIRFFIEAERNNLLLHPEALRLMRSSLRLIDDSFRANPEANQLFLELL
                     TSRNAPETSLRYMNESGVLGRFIPEFGRVVAMMQFNMYHHYTIDEHTIRAIGILSQIE
                     KGGLGEELPLSTEIIGDIQNRRALYLALLMHDVAKACEGDHSVVGAAFTRGLALRLGL
                     SPAEAETAAWLVQHHLVMSQTAQHRDISDPQTIRTFAQIVQSPERLKLLLLLTVADIR
                     AVGPVTWNGWKGQLLRSLYYDTEPLLAGGHTKAPRQARITDAKIRLREALRDWPEQDV
                     ERFIGRHEPDYWLRTDTDRQVKHAHLIRRFEAEDAPLASDVRCCDINHPIPELTFVTR
                     DHPRLLMQCTGACAAAGANIASAQISTTRDGLALDTLLFQRSYYQDNEEIVQTQKIAR
                     VIGEVVSGARSVDAQKVRASRPKPRLGAFTVPPDVVIDNTGSQDCTVVEIQALDRPGL
                     LFDLSRVLSDLDLDISSAHIATFGERAVDVFYVTDSSRKKVTSEEAKQHIRESLLEML
                     SAD"
     gene            complement(3998..5356)
                     /locus_tag="VNR65_08885"
     CDS             complement(3998..5356)
                     /locus_tag="VNR65_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HWJ18827.1"
                     /translation="MHDGRMPNDHRGTRAIQRMVEFAPSTGGLALWVKHHDHPPTSDA
                     PAVTTDGNTLFYGPGFEALPLPEQVGLVAHEVLHIALRHPQRFLELKQLLGDVDLKLF
                     NICADAIVNTSLSHLKWLRLPRGSVYLDELLSNTLGVKPDPQTALLEWDVERLYRTMD
                     DRDDNTQKSGKKDQKRQQSGGSEQSDSGSDKNEQVSASKQTEDRKDGPKASRAKQMGA
                     NILMDLVPTPDPNALPEAEAQHALLWSERVQRSHASDGLHSMLRTLLADLPKVKTPWE
                     QVLRTQLARSLCKKVDISWSRPARSYIANQGRCGDRRMPFEPGYSMAKPTPRLVVIVD
                     VSGSVDDTLLDRFALQIEAITRRQEAGLTLIIGDDRVRKVARYEPGVSDLRNIEFEGR
                     GGTDFTPLLMEAEKHRPDIAVVLTDLDGPARFRPRWPVIWAVPEAYKAAVPPFGRKVV
                     LS"
     gene            complement(5349..>5705)
                     /locus_tag="VNR65_08890"
     CDS             complement(5349..>5705)
                     /locus_tag="VNR65_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18828.1"
                     /translation="FGVLREIQSSVDVVRLMAARPGKDTVALLPRTLDGLYGMTYGLL
                     AACVDDATVARALEIVEQLPDIRSEARLPLREVQTLAMELLMQKALTHGLEGAILESP
                     SYRRYADQRHKEEQYA"
BASE COUNT         1130 a         1650 c         1747 g         1178 t
ORIGIN      
        1 tcaagaaact cgaaaaaatc gaccgcgtcg agccgccgcg tcgccgccag accgtgcagt
       61 tcgattttca gcccgcgccg cgctccggca acgacgtcgt ggcgctcaag gacgttcaca
      121 agcgctatgg aaacagggtg atctatgaag gcctggattt catgatccgg cgcaaggagc
      181 gctggtgcgt gatgggcatc aatggggccg gtaaatcgac gcttttgaaa ctggtggccg
      241 gcgtgagcaa gcctgatcag ggctcggtga cgatcggcgg cagcgtcaag atgggctatt
      301 tcgcccagca tgctatggaa ttactcgacg gcgaccgcac gattttccag tcgctcgaag
      361 atgagttccc acaggcggga caaggcacgc tgcggacact cgcaggctgt ttcggatttt
      421 ccggcgacga tgtcgaaaag aaatgccggg tgctgtccgg cggcgagaag gctcggctgg
      481 caatggcgct tatgttgttc gatccgccga atttcctcgt gctcgacgaa ccgaccaacc
      541 atctcgacat ggcgacaaag gaaatgctga tcacggcgct ggcgcaatat gagggcacga
      601 tgctcttcgt ctcgcatgat cggcattttc ttagcgccct gtccaatcgc gtgctcgaac
      661 tgacccccga aggcgtgcat agctataacg gtggctatgc ggaatatgtc gcacgcacgg
      721 gccatgaagc gccgggcctg cgtggctgat ggcgggcgtg aacggtcttt atagcgaata
      781 agtgttggca gtttgatctc gaatggtgcg ctgaacaaga ctcgagctcc cagctctttc
      841 gcgaacgcat ttcgtgattg aactcggagc tgaggtcggg aattcgacga tcttttagtt
      901 aagttatgtg cgtcaagttc tgcaaaatag ctgggacacc gctcgggtga tgtttctttg
      961 ttcggctgag ggcgtttctc cgtcacggcg gcttggcttg atcgggagtc aaccactccg
     1021 gctccgcact cagccacaga agtatcggca ctggaggtcc gcaatcgtga tgcgtcacgc
     1081 ctgagagcct ggaaattaag tgttcgcgag actggggaac gctgctcagt cggcgctgag
     1141 catttcaagc aggctttcgc ggatgtgctg cttggcctcc tcgctcgtaa cctttttcct
     1201 cgaagagtcc gtgacataga acacgtccac agctctctcg ccgaaggtcg cgatatgagc
     1261 ggacgaaatg tcgaggtcga gatcgctcaa gacgcgcgac agatcaaaca ggaggccggg
     1321 tctgtcgagg gcctggatct cgacaacggt gcaatcctgc gagcccgtat tatcgatgac
     1381 gacgtcgggc ggcactgtga aggctccaag gcgcggcttg ggacggcttg cccgaacctt
     1441 ttgcgcatcg acggagcgcg cgcccgaaac gacctctcct atgacacgcg caattttctg
     1501 cgtctgcacg atctcttcgt tgtcttggta ataggaccgc tggaacagga gtgtatccag
     1561 ggccagcccg tcgcgcgtgg tcgagatctg cgccgaggca atgttcgctc ctgcagcggc
     1621 acatgcgccc gtgcactgca tgagaagcct tggatgatcc cgtgtcacga atgtgagctc
     1681 cgggatcgga tggttgatat cgcagcacct gacatcggag gctagcggag catcctcggc
     1741 ctcgaaacgg cggatgagat gcgcgtgttt aacttgccgg tcggtatcgg tcctgagcca
     1801 gtaatcgggc tcgtgacgcc cgatgaagcg ctccacatcc tgctccggcc aatctcgaag
     1861 cgcttcccgc aagcgaattt tcgcatccgt gatccgcgcc tggcgcggtg ccttcgtatg
     1921 accgcccgcc agaagcggct cagtatcata atagagggac cgcagaagct ggcctttcca
     1981 gccattccac gtgacggggc cgacggcgcg gatatccgca accgtaagca gcaaaagaag
     2041 cttcagcctt tccggactct gcacgatttg cgcgaaagtc ctgatggttt gcggatcgga
     2101 aatgtcgcgg tgctgtgcgg tctggctcat gaccagatga tgctggacaa gccacgccgc
     2161 cgtctcagct tccgcggggg aaagcccgag gcgcagcgcg agaccacgtg tgaaggcggc
     2221 gccgacgacg ctgtggtcgc cttcgcaggc tttcgccaca tcatgcatca ggagcgcaag
     2281 ataaagcgcg cgccgatttt gaatatcgcc aatgatttcc gtcgagagcg ggagctcttc
     2341 tccaaggccg cccttctcga tctgggaaag gatcccgatt gccctgatcg tgtgctcgtc
     2401 gatggtgtaa tggtgataca tgttgaattg catcatcgcc acgacgcgcc cgaactcggg
     2461 gatgaagcgc ccaagaacgc cagactcgtt catatatcgg agagaggtct cgggagcgtt
     2521 tcgcgaggtc agaagctcga gaaaaagctg gttggcctcc gggttcgctc tgaaggaatc
     2581 gtcgatcagc ctcagtgacg accgcatgag ccgaagggcc tcaggatgca gaagcagatt
     2641 gttgcgttcg gcttctatga agaagcggat gagattcagc ggatcttttc ggaaggcatc
     2701 cggctgcttg acactcaggc ggccgttgtc gaaccggaag tcggtcgtcg cagcaagctt
     2761 cgcccgcgag ctccacggga ggacgccata gaaatcgctc aacgtcggca ccgtcttgag
     2821 ctgccttagc tccagactcg agcagatcgt ccgcgtgaga tccccgacgt ctttcgcgac
     2881 gaggaagtaa tgcttcatga aacgctcgac gccgagaagc cccttgcggt cgtggtagcg
     2941 caggcggctt gccatctcgg gctgcaggtc gaaggacaaa cgctcatcgg gtttgccggt
     3001 gagaaaatgg aggtgacagc gaattgtcca gaggaaggct tcgcagtggt ggaaggtcga
     3061 gacttcctgc ggcgcgaaga ccttgttctc ggcctggcca ttcttgccag ggttgaggta
     3121 ggccgagagc caatgcagca tgttgaggtc gcgcaacccc cctttgccct ccttgatgtt
     3181 tggctcgact ctgtaccgcg agacgcctgc tcgtctgagc cgctcttccc tttccgcaag
     3241 tttcgcctcg atgaagtcgc gctgcgagcc ttgtacgaca cttgcgatga agcgcgtcca
     3301 tagttcccgg aacagcgcgc cttcgccgta aaggagccgc gcatcaagca acgcggttct
     3361 tatcgtcatg tcctccctgg cggccttcaa agtctgatcg accgtgcggg tggcatggcc
     3421 aaccttgtag ccgaggtccc aaagaaaata gaggatggat tcgacgatgc tctcgcccca
     3481 tgccgtttgc ttatagggca gaagaaaaag cagatcgacg tccgatgagg gcgccatcat
     3541 cccgcggcca tatcctcccg tcgcgacgat cgccatgtgc tctgccgcgg atggattttg
     3601 ggcccggtaa agatgcttgg tggagtaatc gtaaatgagc ttaatgagct catcctgaaa
     3661 ctgggctaga gacctggcgc aagcgcaccc atcgcccgtt cgcataagca gtgcttgtgc
     3721 ttccgttcga gcgttgtact tcagctcctt gagacgaccc accacacgcg ccctggtttt
     3781 tgatggggtg tgggatcctt cccgccagta ggagtcaaac tcctcacgaa gagcagccgg
     3841 gtcgaaataa ggcggagttg caaaattaac ctgatccatc gcgtctgtct gtctttgcct
     3901 ttcaaccaca acttacatga ttgtgaggct caggcctacc atcacgtcag ggacgcggca
     3961 atagcaactc agggtaggat aatcgctacc gggcggatca actcaggacg actttgcgcc
     4021 caaatggggg aacggcagcc ttgtaagctt ccggcaccgc ccagatgaca ggccagcgcg
     4081 gacggaagcg ggcggggccg tcaaggtccg taagcacgac cgcgatatcg ggccgatgct
     4141 tttcggcttc catgagcagc ggcgtaaagt ccgtgcctcc gcggccttcg aattcgatgt
     4201 tgcgaaggtc cgacaccccc ggctcatacc gcgcgacctt gcggacgcgg tcgtcgccga
     4261 tgatgagagt gagcccggcc tcctgacggc gtgtgatcgc ctcgatctgg agcgcgaaac
     4321 gatccagaag agtgtcatcg accgatccgg agacatcaac gatcacgacg agccgcggtg
     4381 ttggcttggc catgctgtac ccaggttcaa acggcatccg gcgatcgccg caacgcccct
     4441 ggtttgcaat gtaagaccgg gccggccgcg accacgatat atcgaccttc ttacaaaggc
     4501 tgcgcgccaa ttgcgtgcga agaacctgct cccagggcgt tttgaccttt ggcaagtcgg
     4561 caagcaacgt gcgcagcatg gagtgcaggc cgtcgcttgc gtggctgcgc tgcacgcgtt
     4621 cgctccagag aagcgcatgc tgggcttctg cttcgggaag cgcgttcgga tcgggcgtcg
     4681 ggaccagatc catgaggatg ttggcgccca tctgcttcgc gcgcgacgcc ttggggccgt
     4741 ctttccggtc ctctgtttgc ttcgaggccg agacttgctc gttcttatcg ctgccgctat
     4801 cgctttgttc ggagccgccg ctttgctggc gcttctgatc cttcttgccg cttttctgcg
     4861 tgttgtcgtc gcggtcgtcc atcgtccggt acaaccgctc tacgtcccat tcgagcagcg
     4921 ccgtctgagg atccggtttg acgccgagcg tgttcgaaag cagttcgtcg aggtagaccg
     4981 acccgcgagg gaggcgcagc cacttcaagt gagacaagga tgtattgacg attgcgtccg
     5041 cgcaaatgtt gaagagcttg aggtcgacat cgccgagcaa ttgcttcagt tcgaggaagc
     5101 gctgaggatg gcgtaatgca atatgcagca cctcgtgagc gacaaggccg acctgctcag
     5161 ggagcggcag cgcctcgaaa cccggtccat aaaaaagcgt gttgccgtct gtcgtcacgg
     5221 cgggtgcgtc cgaggtcggc ggatgatcgt gatgcttcac ccaaagggcg agaccgccgg
     5281 tggatggggc gaattccacc atccgctgga tcgcgcgcgt accgcgatga tcgttcggca
     5341 tacgcccgtc atgcatactg ctcttccttg tgccgctgat cggcgtagcg gcgataggac
     5401 gggctttcga ggatcgcgcc ctcaagccca tgggtcaggg ccttttgcat caagagctcc
     5461 atggcgagcg tctgcacttc tcgcagcggc aggcgagcct cgctgcggat atccggcaat
     5521 tgttcgacaa tctccagcgc gcgggcgacg gtcgcatcgt cgacgcaggc ggcgagcagc
     5581 ccataggtca tgccatagag cccatcgagc gtcctcggga gcaacgcaac cgtatccttg
     5641 cccgggcggg ccgccatcaa ccgcacgaca tccacgctgg actgtatttc ccgcagcacg
     5701 ccgaa
//
LOCUS       DATMGX010000549         3236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_469585, whole genome shotgun sequence.
ACCESSION   DATMGX010000549 DATMGX010000000
VERSION     DATMGX010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469585"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VNR65_08895"
     CDS             <1..1250
                     /locus_tag="VNR65_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18829.1"
                     /translation="TGAAVHVAGESSDIAFEGVEIWGGGGPGFVLGPNGRGFRITNSR
                     ITRKPDTLLEPGERPRLISLRGDSDARNTRGDILIESSEFAFTEDDGFNIVGVMLQGM
                     AGTEIRSADEIRFVYSGFNPFANLWSSEDVLQMFDPATLKPLSAEPVHVASRVDSLDP
                     ATGTYTFTFRLAMPVPALLAYRGRPVGELPFVADPGHASAPFVLRGNCLRDSSGGRFI
                     VQSGPGLIEGNVTANTGGPGIELGANPVSWHEGPGATDVIVRDNTVIGAGYWLSDYDA
                     RGRLTGLSTGWLAGAGISASALSQTGFMAQGSPNGWLRIVGNHVVNTQGLGLLVAAAH
                     DVVVEGNTVVNANTVPFVAGFDQTRCGARSHGFTLNGAEQPWCLGKVAAQGALMVIDV
                     DRAIISGNVMQGTSKGIFVSRLP"
     gene            complement(1520..1795)
                     /locus_tag="VNR65_08900"
     CDS             complement(1520..1795)
                     /locus_tag="VNR65_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18830.1"
                     /translation="MPVNGKTFRVTRGSGSVADRLHTFMQAHDLGLEELADWLKTHPR
                     TLAQWFDGTAMAPACSLISLSLISRLPQSRIYPSQSKEESLRRVQAI"
     gene            2176..2532
                     /locus_tag="VNR65_08905"
     CDS             2176..2532
                     /locus_tag="VNR65_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18831.1"
                     /translation="MSGSPKLPKEIRTQLGVGAVLAIAGMVWMFIIFFVLKPDQIIPT
                     KDYLSDAYDGMRSITGIPFDQWGLRTIGELVGSFLFLFLMNILYVVARIGWLTLLSLG
                     GFALFEAILQLRKAQR"
     gene            complement(2569..3042)
                     /locus_tag="VNR65_08910"
     CDS             complement(2569..3042)
                     /locus_tag="VNR65_08910"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWJ18832.1"
                     /translation="MIKSAFLIATASFCFAGSAFALDVKKMVDVPAAPEAVWKTIGDY
                     CGIANWHPAIEKCEISKKDGATFRTLSLKGGGTILEKQTAWDDKSRTYSYTIEESPLP
                     VANYKATISVAPKGSGSAIIWTSNFNAKGADDAKASSTIGGIFDAGLASIAAKAK"
BASE COUNT          638 a          888 c          927 g          783 t
ORIGIN      
        1 aaaccggtgc ggccgtgcac gtggccggcg agtcgagcga tatcgcattt gaaggcgtgg
       61 aaatatgggg cggcggcggt cccggtttcg ttttagggcc caacggccgc ggcttcagga
      121 tcaccaacag ccggatcacc cgcaagcccg ataccttgct cgaaccgggg gaacggcctc
      181 ggctgatctc tctccgtggc gattcagatg cgcgcaatac gcgaggcgat attctgatcg
      241 agagcagtga attcgcgttc acggaggacg acggtttcaa catcgttggc gtcatgttgc
      301 agggcatggc ggggactgag atccgttctg ccgacgagat ccgctttgtc tatagcgggt
      361 tcaacccgtt cgccaaccta tggtcatcgg aagatgtctt gcagatgttt gatcccgcaa
      421 cattgaagcc gctgtcggcg gagccggtgc acgttgcttc gcgcgtcgat agccttgatc
      481 cggcaaccgg aacttacact ttcaccttta ggctcgcgat gccggtgccc gctctcctgg
      541 cctaccgcgg acgacctgtt ggtgaattgc cgttcgtggc cgatcccggg catgccagcg
      601 ctcccttcgt acttcgcggt aactgccttc gcgacagctc tggtggccgc ttcatcgttc
      661 agtcgggtcc gggactgatc gaagggaatg tgacggcgaa cactgggggc cctggcatcg
      721 aactcggagc caaccccgtg agctggcacg aaggccctgg cgcgacggac gtcatcgtgc
      781 gcgacaatac cgtaattggt gctggctatt ggctgtctga ctacgatgcg aggggcaggc
      841 taacaggcct gtcgaccggt tggctcgcgg gcgctggcat cagtgccagt gctctgtccc
      901 aaactggatt catggcgcaa ggctcaccta acggctggtt gcgcatcgtc ggtaatcatg
      961 tggtgaacac ccagggcctc ggacttcttg tagcagcggc ccacgacgtg gtggtggagg
     1021 ggaataccgt cgttaacgcc aacacagttc ctttcgtcgc cgggttcgat caaacgcgct
     1081 gtggggcgcg ttcacatggc ttcaccctca acggcgccga gcagccctgg tgcttgggca
     1141 aggttgcggc acagggtgct ttgatggtca tcgatgtcga ccgcgcgatc atctccggca
     1201 atgttatgca gggaacaagc aagggaattt ttgtcagcag acttccctag acggagatct
     1261 gccccaggcc cgatcaagag gctcatattg acaagctgat cgtgcggagc cccgttcgca
     1321 ggaggtgctt agcccgccta tgcatcccta ccttgaaccg gcttagcagg accgcgcgat
     1381 ggacgccatc gtcaagtcga tgcgaaggcg cccgcgttag gcaataagcg ttcgtcggag
     1441 ttccgaatga aatgcgaaga gccgccttgg tgccgaggcg gctcttttgc gttcaagctc
     1501 acgtacggag caagcgtcct tagatcgctt ggactctcct caacgattcc tctttgctct
     1561 ggctggggta aatgcgcgat tgcggcaggc gtgagatcag ggacagtgaa atcagcgagc
     1621 aggctggggc catcgctgtc ccatcgaacc attgagccaa ggttcgcggg tgcgtcttaa
     1681 gccaatcggc aagctcctcg agtcccagat cgtgggcctg catgaaggtg tgcaaacgat
     1741 cggctacgct tccgctacct cgcgtgacgc gaaaggtttt cccattcacg ggcatcagtt
     1801 tcctcccagg ctttttttca atgcggatgc ttcggatcgt caccgctgtt aatcatttat
     1861 aacggaattg cttcgggagg tcactcacat tcggtttgtt gctcgaaccc agctttctct
     1921 tgcgttgaag agagcgcccc tatgtgcgcc aaactctgga tgccagaccc gcgccaccaa
     1981 tcatgataaa cgccattcca agttagaacc ttgaacggaa cgagtatcaa ttggctatca
     2041 ccaaattgtt ttctatgtat cagcttgata tatgcgttct gaaacccatg atagtctgta
     2101 aataggtctt tatcggtcga tcagagttag aaaagtcggc ccttatgaaa cgacgctata
     2161 tttgcaggag ctttcatgtc cggttcgcca aaattaccga aagaaattcg cacgcaattg
     2221 ggagtcggag cggtccttgc catcgccggc atggtttgga tgtttatcat ctttttcgtt
     2281 ctgaagccgg atcaaatcat tccgacaaaa gactatctga gcgacgccta tgatgggatg
     2341 cgctctatta ccggcatccc ttttgaccag tggggcctga gaacaatcgg agagttggtt
     2401 ggaagctttc tttttctctt tcttatgaat attttgtatg tggttgcccg tattggttgg
     2461 ctcacattgc tttctctggg aggctttgcg cttttcgaag ccattttaca acttagaaaa
     2521 gcgcagcgct aagcacatct tctcggcaaa ggcgagtgta atcttcggtt acttcgcctt
     2581 tgctgcgatg ctagcaaggc cagcatcgaa aatcccgccg atcgtggatg acgccttcgc
     2641 gtcgtccgcg cccttcgcgt tgaaattgct ggtccagata atggccgatc cacttccctt
     2701 cggcgcgacc gagatcgtcg ccttgtaatt tgcgaccgga agcgggcttt cctcgatggt
     2761 gtagctgtag gttctggact tgtcgtccca ggcggtttgt ttctcaagga tggtgccgcc
     2821 gcctttcaag gaaagcgtgc ggaatgttgc gccgtctttc ttggagattt cacatttttc
     2881 gattgccgga tgccagttcg cgatcccgca ataatcgccg atggttttcc agacggcctc
     2941 aggcgctgcc ggcacatcga ccattttctt gacatcgagc gcgaaggcgg agcctgcgaa
     3001 gcagaagctc gcagtagcaa tcaagaaagc tgattttatc attgttccct ttggggttag
     3061 acgtttcctt ccggccccat aatgcctcaa ggatgcctgt ttacacatta acccttaggc
     3121 ggagcctccc tgagcggccg cgcggatctc accgaggctt tggcgcggag taatcgcttc
     3181 cggatcgatg cgaagctcta ggatggatgg cactcccgac gcttgggccg cttcga
//
LOCUS       DATMGX010000550         2050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_91229, whole genome shotgun sequence.
ACCESSION   DATMGX010000550 DATMGX010000000
VERSION     DATMGX010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91229"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VNR65_08915"
     CDS             complement(<1..577)
                     /locus_tag="VNR65_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18833.1"
                     /translation="MALAASSISVSTTKDPVAAIAQLAREASIEITVHDIMHLEESRS
                     LLPAGKSLFVSHLPKQQWRETEAACRAVRAAGFRPVPHVPVRLLPDAATADLVLGGFV
                     KAAQVDEVLLISGDYRQALGPYTEVLQLMQNGMLQRHGMRRVTVAGHPEGHPKVKLAD
                     IRRVEVDKARMAQQAGLELTFLTQFFFEQRPF"
     gene            complement(590..1963)
                     /locus_tag="VNR65_08920"
     CDS             complement(590..1963)
                     /locus_tag="VNR65_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HWJ18834.1"
                     /translation="MKGAGNTVEMLRNSRIGAYVYPVVPSEFYNWRDEQRAWRETAVL
                     FDQTHHMAELTVKGPDALRLMSYTTINSFNNFTVNKAKQMVPVSYDGFVIGDGILFYL
                     AKDELLFVGRAPSVNWIQFHGETGGFKVDMIRDDRSPSHPRGKAVTRRHYRYQVQGPN
                     AAKVLEKLNGGPLPDVKFFTMDAINIKGRKVRALRHGMAGAPGLEVWGPYAEGDEIRE
                     AILEAGREFDLVPVGSRAYASNTLESGWIPSPLPAVYSGEKMKKYREWLPAGGYEGTG
                     SIGGSFISKNIEDYYMTPYELGYGPFIKFDHDFIGREALEKKSKDPHRKKVTFAWNGE
                     DCATIYASLFMPGTDNYKFLDIPIANYASSSYDSVMMGGKVVGFSMFGGYSYNERTAL
                     SLGVVDANINVGDVLTLVWGEEGGGTKKTTVERHKQLDVRVKVAPVPYARDAREGYHA
                     GWRTRQS"
BASE COUNT          408 a          649 c          553 g          440 t
ORIGIN      
        1 gaaaaggccg ctgctcaaag aagaattgcg tcaaaaaagt cagctccaaa cccgcctgct
       61 gcgccattcg cgccttgtcc acttcaacac ggcggatgtc cgcgagtttt accttggggt
      121 gcccctcggg gtggcctgcg accgtgacac gtctcatccc atgccgctgc agcatgccgt
      181 tctgcattaa ctgcagaact tctgtgtaag gtcccagcgc ctgccggtaa tcgcccgaga
      241 tcagcagaac ctcatcgacc tgcgcagcct tcacaaaacc gccgagcacg agatcagcgg
      301 tcgccgcatc gggcagcagt cgcacaggta catgtggaac cggcctgaag cccgcggcgc
      361 gcaccgcgcg acaggccgcc tcggtctcgc gccattgctg cttcggcagg tgactgacaa
      421 agaggctctt gcccgccggc aacaggctgc ggctctcttc caggtgcatt atatcgtgca
      481 cagtgatctc aattgacgct tcgcgcgcca gctgcgcaat ggctgccacc ggatctttcg
      541 tggtcgagac cgagatgcta cttgctgcca gcgccatgat tggtgccaat cagctctgcc
      601 gcgtgcgcca gcccgcgtga tagccctcgc gtgcgtcacg cgcatagggc accggcgcca
      661 ctttcacccg cacatcgagc tgtttgtgcc gttcgaccgt ggttttcttc gtgccaccgc
      721 cctcttcgcc ccacaccagt gtaagcacgt cgcccacatt gatattcgca tccacgacac
      781 cgagtgacag cgccgttcgt tcgttgtagc tgtagccgcc aaacatcgag aaacccacga
      841 ctttgccgcc catcattacg ctgtcgtacg aggacgatgc gtagttggca atgggtatat
      901 cgagaaactt gtaattgtcg gtgcccggca tgaacaaaga ggcgtaaatc gtggcgcaat
      961 cctcgccatt ccaggcaaag gttaccttct tgcgatgcgg gtccttcgat ttcttttcca
     1021 gcgcctcgcg tccgatgaaa tcatgatcga actttatgaa cgggccgtag cccaattcgt
     1081 agggcgtcat gtaatagtct tcgatgttct tggagatgaa gcttccaccg atcgaaccgg
     1141 tgccttcgta acccccggcc ggcaaccact cacgatattt cttcatcttc tcgccgctat
     1201 acaccgccgg caggggcgac ggaatccatc cggattccag cgtattgctg gcgtaggcgc
     1261 gcgagcccac cggcaccaga tcaaattcgc gtccggcttc caatattgcc tcgcgtattt
     1321 catcgccctc ggcataggga ccccaaacct ccagcccggg agcacccgcc atgccgtgac
     1381 gcagggcgcg caccttgcga ccctttatgt tgatggcatc catggtgaag aatttgacgt
     1441 cgggaagcgg tccgccgttg agcttttcca gcactttggc ggcattcggc ccctgcacct
     1501 ggtaacgata atggcgccgc gtaaccgcct tgccacgcgg atgcgagggc gaacgatcgt
     1561 cgcgtatcat atccaccttg aagccgccgg tctcgccgtg aaattgaatc cagttcaccg
     1621 acggcgcgcg gccgacaaac agcagctcgt ccttggcaag atagaacaga atgccatcgc
     1681 cgatgacaaa gccgtcgtag ctgaccggca ccatttgctt ggccttgttc acagtgaagt
     1741 tattgaagct gttgatggtt gtatacgaca tgagccgcaa ggcatcaggg cccttcacgg
     1801 tcagctccgc catatggtgc gtctggtcga acagcacggc agtctcgcgc caggcgcgct
     1861 gctcatcgcg ccaattatag aactcgctgg gcaccaccgg atacacgtag gcgccgatac
     1921 gcgaattgcg cagcatctcc accgtgttgc cggcgccctt caaaagagtt tcgagatttt
     1981 gctgactcat gatggtgatg tcctcttgtc tgatccgatt gctattgatt taccacggtg
     2041 cgccggagct
//
LOCUS       DATMGX010000551         3509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_287314, whole genome shotgun sequence.
ACCESSION   DATMGX010000551 DATMGX010000000
VERSION     DATMGX010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287314"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VNR65_08925"
     CDS             <1..833
                     /locus_tag="VNR65_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWJ18835.1"
                     /translation="AEGAQMAVSETGQWSGYISGGCLEAAIALEAVAAMKAGKPRLLR
                     YGKGSPYLDIRLPCGSGLDVFIQPIRDAALIEDMSHRLERRRPFALRIDLATGACQLE
                     ARAGEALLSRREGSAFFRAYEPALRCLIIGSSPIAVALAELAACAGFEAEFYATDPEI
                     LPKLPVSVKVQPLNPRVPFAADRWTAAILAFHDHDQEFPFFSSLLPSPCFFIGAIGSR
                     NAHAVRQDVLTEAGFSEAEVARINSPAGLVTGLKTAPCVALSILTQIVAAAREQGIVC
                     "
     gene            827..1426
                     /locus_tag="VNR65_08930"
     CDS             827..1426
                     /locus_tag="VNR65_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWJ18836.1"
                     /translation="MLEKRSRFGLIMLAAGLSRRMGGPNKLLQAYLGKPLLAHALKIA
                     EEIEFFDRVAVTGRDCAETEALAAPFGFRCVFNPSFAEGLGTSIAAGARALHPEAEGA
                     FIALGDMPEIGPDVYRALSEYFTQGSIVAPLHNGERGHPVLFCKSYIAQLSVLSGDEG
                     ARSILRRHTLSVAEVETANPGVLRDVDTREDFYCSGAEN"
     gene            1573..2007
                     /locus_tag="VNR65_08935"
     CDS             1573..2007
                     /locus_tag="VNR65_08935"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWJ18837.1"
                     /translation="MQNMPNFAELPDSMRNIMKSSIEQARKAFDTFVASSEKMMQSLD
                     TSSIPAADGVKQLNEKIAAFTRQNAESNFNLAMQLTDAKHLSEIVELQNAHLRTQMEN
                     FSRQMEELRELTMKAVKDGTKAASSAVQNAASSMPSNPFTGN"
     gene            2212..2454
                     /locus_tag="VNR65_08940"
     CDS             2212..2454
                     /locus_tag="VNR65_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18838.1"
                     /translation="MKKNKRREPAIIRAWAEEEDKKITIQIEDGDGETLAFIQLTPDK
                     ASEFSDCLAIYAAKLKMASREKSASRNTSMRPALLC"
     gene            2539..2615
                     /locus_tag="VNR65_08945"
     tRNA            2539..2615
                     /locus_tag="VNR65_08945"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2573..2575,aa:Pro,seq:cgg)
     gene            2824..>3509
                     /locus_tag="VNR65_08950"
     CDS             2824..>3509
                     /locus_tag="VNR65_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18839.1"
                     /translation="MRRAITAIVVVALLQSATAAPPPANGPWPEVAQRWIKKYRTKPE
                     PLAVPAVIRGLSEHGTLKEPESSGLYVGFVAGVLGSNPKKAWTLIDKTLPLPFEDQWL
                     LIRAVAYSGLPEWKDHMRGLAMRLPDRQEMVQRYLSGDLPPLSEVALEKQRPGTFDKV
                     KSFFNGDMFFKANAEPPKRQITFESNADLMDTLWGIYFATGKEAPIAQIVTLLPWTKE
                     RDSVEKLTIG"
BASE COUNT          735 a         1022 c         1019 g          733 t
ORIGIN      
        1 gagccgaggg agcccagatg gccgtgtctg aaaccggaca atggtcgggt tacatttcgg
       61 gcgggtgtct cgaagcggcc atagcgctgg aggcggtggc agcgatgaaa gccgggaagc
      121 caaggcttct ccgctacggg aagggatcgc cctatctcga catccgcctg ccatgcggga
      181 gcgggctcga tgtcttcatc cagcccatac gagacgcggc cctcatcgaa gatatgagcc
      241 atcgcttgga gcggcggcgg ccgttcgcac ttcggatcga tttggcgaca ggggcgtgtc
      301 agctggaggc gcgagccggt gaagcgcttc tatctcgccg cgaggggagc gccttcttcc
      361 gcgcttatga gcccgcgctg cgatgtctga tcatcggctc aagcccgatc gctgtggcgc
      421 ttgcggaact ggccgcttgt gcgggctttg aggcagaatt ttacgcgacg gatccggaaa
      481 ttttgccaaa gctgccggtg tccgtgaagg tgcagcccct caatccgcgc gtacctttcg
      541 cagcggatcg ttggacggcc gcgatcctcg catttcacga ccacgaccag gaattccctt
      601 ttttctctag tttgcttccg agcccctgtt tcttcatcgg agcgattggc agcagaaacg
      661 cacacgcggt ccggcaggac gtacttaccg aggcgggctt ctcagaagct gaggttgctc
      721 ggatcaacag tcctgcgggc cttgtgacag ggctgaagac cgcgccctgc gttgccctgt
      781 ccattctgac gcaaattgtc gcggctgccc gggaacaggg gatcgtttgc tagagaagcg
      841 ctcgagattt ggactcatca tgctggcggc gggcctctcg cggcgcatgg gtggcccgaa
      901 caagctcttg caggcctatc tcggaaagcc tctgcttgct catgcgctaa aaatcgctga
      961 ggagatcgag ttcttcgatc gtgtagccgt gacgggacgc gattgtgccg agaccgaagc
     1021 cctggccgcg cctttcggct tccggtgcgt tttcaatccc agcttcgccg aaggcctcgg
     1081 aacctccatc gcggccggtg cgcgtgcctt gcatcctgag gccgaaggtg cctttatcgc
     1141 attgggcgat atgccggaaa ttggcccgga cgtttatcgc gctctgtctg agtatttcac
     1201 gcaaggatca attgttgcgc ctctccataa cggagagcgg ggccacccgg tcctcttctg
     1261 caaatcctat atcgctcagc tttcggtttt gtcgggcgat gaaggcgcgc gttcgatcct
     1321 gcgccgtcac acgctctctg ttgcagaggt tgaaaccgca aatccaggtg tactacgcga
     1381 cgtcgataca cgcgaagatt tttactgcag cggggccgaa aattgaaaat ttagttcgct
     1441 ttttcaagat cccataagca tattgtgaag cttgtcaatt cggccttcat ctccaatact
     1501 tgatgtacag ccgtacgcag aatagccaca cggttctcgc cgtttcacat cgatcaggcc
     1561 agggagatcg ccatgcagaa catgccaaat ttcgcagaac ttcccgattc catgcgcaat
     1621 atcatgaagt ccagcatcga gcaggcgcga aaggcgttcg acaccttcgt cgcctccagc
     1681 gaaaaaatga tgcagagcct cgacacgtca tcgatcccgg ctgccgatgg tgtgaagcaa
     1741 ctgaatgaga aaatcgctgc gttcacccgc cagaatgcag agtcgaattt caacctcgcg
     1801 atgcaactca cggatgcaaa acacttgagc gagatcgtcg agcttcagaa cgctcatttg
     1861 cggacccaga tggaaaattt cagccgccaa atggaagagc ttcgcgagtt gactatgaaa
     1921 gcggtcaagg atggaacgaa ggcagccagc tcggcggtgc agaatgcggc cagttccatg
     1981 ccgtccaatc ctttcacggg taactgattt ttagctttgt cttggctaaa atctaggcaa
     2041 atgcttcatc tatgagcaga aggcgctcca aggaggaggg gagcgccttt ttcgatacgg
     2101 tcccgcacgg ttttgacgcg gaatcggcac aatgattgct tattcttacc cggtggggtc
     2161 gaacggctca gaccgaacgg ccttggtgat ctgagttgga ggagcagggc tatgaaaaaa
     2221 aacaagaggc gggaaccggc gatcattcgc gcctgggccg aggaagaaga caaaaagatc
     2281 accatccaga tcgaagacgg cgacggggag actctggctt tcatccagct cacccccgat
     2341 aaggcgtccg aattctccga ttgtctcgcc atttacgcgg cgaagttgaa gatggcttcg
     2401 cgcgaaaaga gcgcttcgcg caacacatcg atgcgccctg cgcttctctg ctgaaaaagg
     2461 agcttcagct tctgaataat cgttctctca tgcttgcgcg cggcgccgtg acaggtatat
     2521 acagggctct cacggcgtcg gagtgtagcg cagcctggta gcgcacctcg ttcgggacga
     2581 gggggtcgag tgttcgaatc actccactcc gaccaatctt ttcagctcaa ttgccgtact
     2641 ccgcccctcc aagaggggcg ttttgctgcg tgctcctccc gatccatctc cgatccacca
     2701 agaccaattg gggcatcatc gcccgttaac cttcataaaa aggccacgca cacgcgctta
     2761 agttcttgac gtgtatccgt ccggccaccg gccgccgggc atctcggtca gcgagaggag
     2821 tccatgcgcc gcgccattac agccatcgtc gtcgtcgcgc ttttgcaatc tgcaacggcc
     2881 gcgcctcccc ccgcgaacgg tccctggccc gaggtcgccc agcgctggat caagaaatat
     2941 cgcacgaaac ccgagcccct ggcggtgcct gcggtgatcc gcggcctgag cgagcacggc
     3001 acgctgaaag agccagagag ttcgggcctc tatgtgggct ttgtcgcggg cgtgctcggc
     3061 tcaaacccga aaaaggcgtg gacgctgatc gacaaaacgc tgccgctgcc cttcgaggac
     3121 cagtggcttt tgatccgagc cgttgcttat tcgggcctgc cggaatggaa ggaccatatg
     3181 cggggcctcg ccatgcggct acccgatcgc caggagatgg tgcagcgcta tctgtccggc
     3241 gatctgccgc cgctcagcga ggtcgcgctc gaaaagcagc gcccgggcac atttgacaag
     3301 gtgaaaagct tcttcaacgg cgacatgttt ttcaaagcca acgctgagcc tccgaagcgc
     3361 cagatcacct tcgagtcgaa cgccgacctc atggacactc tttggggcat ctatttcgcg
     3421 acgggcaaag aggcgccgat cgcacaaatc gtgacactgc ttccttggac gaaagagcgc
     3481 gacagtgttg aaaagctcac catcggaag
//
LOCUS       DATMGX010000552         9268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_68021, whole genome shotgun sequence.
ACCESSION   DATMGX010000552 DATMGX010000000
VERSION     DATMGX010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9268
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68021"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VNR65_08955"
     CDS             <1..266
                     /locus_tag="VNR65_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18840.1"
                     /translation="HNLMNNRQLQHGHSLAFSELRHEHVASVWKFNRIMVTMRNIRVY
                     RAEFPHPEIGCSRPKPSVVVFDILGECQFGPRKHADRCGGLTF"
     gene            960..1325
                     /locus_tag="VNR65_08960"
     CDS             960..1325
                     /locus_tag="VNR65_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18841.1"
                     /translation="MADQSARKRWILTHVTRVWTALFTLASLGAAFVYKPEFLTLYLR
                     TTMTAIETGTAALPYPWGDRLEVVLRGIGGSVWMQFAFAIVVVRVIVWLIARFIGYCW
                     RHIRRDPVSIMVDRRMDRR"
     gene            complement(1523..1765)
                     /locus_tag="VNR65_08965"
     CDS             complement(1523..1765)
                     /locus_tag="VNR65_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HWJ18842.1"
                     /translation="MLYPAPGLPDETPIDNIRFSTRIRSALNAAGLKTVGEIREASDA
                     TLLSFRDLGAGSIARLRETLGLPPARALSPKGKKPT"
     gene            complement(2154..2366)
                     /locus_tag="VNR65_08970"
     CDS             complement(2154..2366)
                     /locus_tag="VNR65_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWJ18843.1"
                     /translation="MAESVYKVIELIGTSKDSWEKAAAAAVDRAGKSLRDLRVAEVVL
                     LDLQLDAAGKVEAYRAKLKISFKFEG"
     gene            complement(2570..3103)
                     /locus_tag="VNR65_08975"
     CDS             complement(2570..3103)
                     /locus_tag="VNR65_08975"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ18844.1"
                     /translation="MLAKYMTAGLAASALLATVAFAQTPTTTTDRANMAAAEASDSSF
                     QGDWRTSKIVGLNVYNDKNESVGSINDLLTDKNGNIKAVVIGVGGFLGVGEHLVAVAF
                     DKIKFVNEPVAYTGASNAPAPGSRPASSTTTGAAPTNTAPATATASKPNPWYPDHALF
                     NATKDELKALPEFKYST"
     gene            3327..3692
                     /locus_tag="VNR65_08980"
     CDS             3327..3692
                     /locus_tag="VNR65_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18845.1"
                     /translation="MQRQALVLILATLTASSAATAQAPPTPATPPAQTAPPAPARNAT
                     GCAPTQSMPQGTIAPQGTTTTDPRAEPLSDKLARSDGVLCPPTGVDPEIRAPTPETGN
                     MPVIPPPGSPGGDPTIRPK"
     gene            complement(3776..4024)
                     /locus_tag="VNR65_08985"
     CDS             complement(3776..4024)
                     /locus_tag="VNR65_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18846.1"
                     /translation="MTDDVPPPPFIYEGAETIEDVKSPATGVSDTVKATAHRLSDAYD
                     TARKPGMPLSILSNIAREAPLGSLLVAFLLGVAVARRR"
     gene            4223..4450
                     /locus_tag="VNR65_08990"
     CDS             4223..4450
                     /locus_tag="VNR65_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18847.1"
                     /translation="MGDKFNPAPIDKHAASPKEAAKADKATHNELQKGLKDTFPASDP
                     VSATQPAPSKHDSDAKKKAGSVESGNKSTRK"
     gene            5167..6698
                     /gene="ltrA"
                     /locus_tag="VNR65_08995"
                     /pseudo
     CDS             5167..6698
                     /gene="ltrA"
                     /locus_tag="VNR65_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            6790..7821
                     /locus_tag="VNR65_09000"
     CDS             6790..7821
                     /locus_tag="VNR65_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ18848.1"
                     /translation="MQTITTIGLDIAKSVFQVHGVDAGGQVVIRRQLKRRYVLAFFQK
                     VPPCLVGIEACASSHHWSRELKALGHNVRLIPPAYVKPYVKRQKNDAADAEAICEAVT
                     RANMRFVETKTPEQQSGMVLHRTRHLCMRQQISVINAIRAHFAEFGIVAAVGRKGVEE
                     LLNIVADPSDKRVPEIARTCLAALGTQLRRLKEQILEFDRMIRAWHRSSEMSMRLDVA
                     PGVGPVLATALVATVADPNAFRSGRNFSAWIGLVPKQHSSGGKNRLGSISKQGDRYLR
                     GLFVIGALAVIRYAKIHGTKHRPWLTALLARKPAKVAAIALANKMARMVWAMMARGER
                     YKEPVALAR"
     gene            8262..>9268
                     /locus_tag="VNR65_09005"
     CDS             8262..>9268
                     /locus_tag="VNR65_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ18849.1"
                     /translation="MQTITTIGLDIAKSVFQVHGVDAGGQVIVRRQLKRRYVLTFFQK
                     LSPCLVGIEACASAHHWSRELQALGHLVRLMPPAYVKPYVKRQKNDATDAEAICEAVS
                     RANMRFVPTKTPEQQSGLVLHRTRHLFIRQQTSAINAIRAHLAEFGIVAPVGRHGVEE
                     LLTVVADPNDRRVPEIARACLSALGAQLRRIKEQILEFDRLIRAWHRSNEMSMRLEEA
                     PGVGPVLATALVAAVADPKSFRSGRNFSAWIGIVPKQHSSGGKNRLGNISKQGDRYLR
                     GLFVAGALSVIRYAKIHGTKHRPWLTALLARRPTKVAAIALANKIARMVWAMMARGER
                     Y"
BASE COUNT         1898 a         2731 c         2835 g         1804 t
ORIGIN      
        1 ttcataacct gatgaacaac aggcaattgc agcacggtca ctcgctggcc ttcagcgagc
       61 tgcgtcacga gcacgttgct tccgtctgga aattcaatcg catcatggtg acgatgcgaa
      121 acatccgggt ctatcgcgcc gaatttcccc acccggaaat tggttgttct cgtccaaagc
      181 catctgttgt cgtatttgac atcctcggcg aatgccagtt cggtcccagg aagcatgcag
      241 accgctgcgg tgggctcact ttctgatgcg aaaccgcgag tcgcagttcc gtagaatgtc
      301 gtggtgatca gtgtctctcc aacttgggcg gggcgcgttg ctacagcgtg cagactatag
      361 tcacacatcg gattgctccc tcgtttgaga tagccaaccc gcgcccgtta gaggcgcagg
      421 cctctatcag agtggacagt agcccgcgag cttttgcttc attgtgggcg attgtacggc
      481 ctgggccgat cgccctgatc acaaatacgt ttgacgaggc atccaatcct tgccacctgc
      541 cggcgctcag gactcgtttc cacggtggcc tgcgattcgg gcagccttca aagcagaaac
      601 gaaccaatca cgtctctgga ggtcactgag ctttaggtgc cagccaaaga cggaggcggg
      661 cgaaaactgc catgccgccg agaaatcccg ccgccccggt cggttagctt cggcggaaga
      721 atttggtcca cctgcaaaat atgcgcctac ggcgcactgg atcaagccag tctcgctgtc
      781 ttatcaccgc ccgccggctc tccccggcgg gtttttcttt ccccgccaat gcaaccattg
      841 agctgctcgt tgattacatt gatagcggcg gaaccgggaa accggcagtc gcgtattaga
      901 gccgcacgga gtaatgcccg ggcggctttt tctatgccgc cgccgaacag gatccgagga
      961 tggccgacca atctgcgagg aaaaggtgga tcctcaccca tgtgacgcga gtgtggaccg
     1021 ccttgttcac gttagccagc cttggcgccg ccttcgttta caagcccgaa tttttgacct
     1081 tgtatcttcg gaccaccatg acggcgattg agacaggaac ggccgctctg ccgtatccct
     1141 ggggcgatcg tctagaggtg gtcttgcgag gcattggtgg gtcggtttgg atgcagtttg
     1201 cattcgccat cgtggtcgtc cgcgtgatcg tgtggctgat cgcgcgattc attgggtact
     1261 gctggcgcca catacgtcgg gacccagtat cgataatggt cgaccggcgg atggatagac
     1321 gttgaggcac cccggccttc gggattcgtt ttccgcgatg gccggcgggg gtcggagcct
     1381 ccgacgccta gaattcacca gcgggcttcc tggagtcatg gcgggaataa ttcccgacaa
     1441 cgctagcgct cgaaggcgtt catcttcctc aagcgttcag cccgcgcgaa agccgccctg
     1501 agggcttggc aatttgctgt aatcaggtcg gttttttacc ctttgggctc agcgcgcgtg
     1561 ctggtggcag ccccagagtt tcgcggaggc gggcgatcga gccagctcca aggtctcgaa
     1621 agcttagcag cgtcgcgtcc gacgcctcgc ggatttcacc gactgtcttc aagcccgccg
     1681 cgttgagcgc gctcctgatc ctggtggaaa atcggatgtt gtcgataggc gtttcgtccg
     1741 gaagccccgg agctggataa agcatcgaag ggttctgttt atctcaacat atcggtaatc
     1801 tcgatccggc aaataaccga tcatcgtata agcccgcagc agacttccaa atcgcgaccg
     1861 atacatgctt gacgagggca tgttgtctcg ctcgtcaatg acgtgaccgg aaagtgaacc
     1921 ctcctcttca agtaccgctt gcagcaacgc gagcagttcc gcatcggaat aatgattgtc
     1981 gcgctgatcg attattgttc gcgcgcggtc gaatagcgcc tgatctacta ttgaaggaaa
     2041 ggctccctcc gcgcgaaccc aggtttcagc tcgtattctg gtatgcgtga tcttccccgg
     2101 acagccttgc cgcctgcggg agagggagcg caaggcgcat cgcgatcgcg tcgtcagccc
     2161 tcgaatttga acgatatctt gagcttggcg cggtaggcct cgaccttgcc tgcggcatcc
     2221 agttgcaggt cgagcaggac gacttcagcg acacgaagat cccgaaggga cttgccggcc
     2281 ctgtcgaccg ccgccgcggc ggccttttcc caggagtcct tgctggtacc gatcaactcg
     2341 atgaccttgt agacgctttc agccatgtcg tcctcccgtt cggtgtgagg caggacgcag
     2401 gtggtcctgc ggccgggaag cctaacaccg ggatatcggc gctgaaagga tgcggcgcac
     2461 catgccgctg cctcccgtgc gcgacgcgcc gtatgcggtc aaaaaaatga ccggacggca
     2521 gcaatgccgt ccggccattt gaagatcagg tctaactcac gccggcgcct tacgtcgaat
     2581 atttgaactc aggcaacgcc ttcagctcat ccttggtcgc gttgaacagc gcatgatccg
     2641 gataccaggg attgggcttc gaagccgtcg ccgtggccgg tgcggtattc gtgggcgcag
     2701 cacccgtggt cgtcgatgac gccggccggc tgcccggggc gggggcattc gacgcaccgg
     2761 tgtaggcgac cggctcgttg acgaatttga tcttgtcaaa ggccacggcg accagatgtt
     2821 cgccgacgcc gaggaaaccg ccgacgccga tcaccaccgc cttgatgttc ccgttcttgt
     2881 cggtcaggag atcgttgatc gagccgacgc tctcattctt gtcgttgtag acgttgaggc
     2941 cgacaatctt cgaagtgcgc cagtcaccct ggaacgagga atccgatgcc tccgccgccg
     3001 ccatgttggc gcgatcggtg gtggtcgtcg gagtctgcgc aaacgcaacg gtcgcaagta
     3061 acgcggacgc ggccaagccg gccgtcatat attttgctaa catgtgattt ctccctgata
     3121 tgaaatagat agggaggaac acgccactcc gccagctgtt ccaggcggcc ggacagattt
     3181 tcagcggccg cgtgactcgc ggttggcggg ttcccctgaa acgccgccgt tcgctcgctc
     3241 ggcatcctga gggccggtag accgaatacg ccagtcggtt tcgccgccag cagggaacaa
     3301 cggtgacgcg gaagcatttg ctccccatgc aaagacaagc gcttgttttg atcttggcaa
     3361 ctctgaccgc atccagcgcg gcaaccgcgc aggctccgcc tacgccagcg acgccgccgg
     3421 ctcaaacggc cccgccagcg cccgcgagga atgcgacggg ttgcgcgcct acgcaatcga
     3481 tgccacaagg caccatcgcg ccgcagggca ccacgaccac cgacccgcga gcggagccgc
     3541 tcagtgacaa actggcgaga tccgacggcg tgctctgccc gcccacagga gttgacccgg
     3601 aaattcgcgc accaacgcct gagacgggca atatgccggt cattcctcca cccggcagcc
     3661 ctggcggcga tccgacgatc cggccgaaat aaatccggcg ttgcggcgac ctcacgacga
     3721 gggggcctag tttgcggttc gaataaagag ccgccttcgg gcggtctttt gtttgctaac
     3781 gccgccgcgc caccgcaacc ccgagaagaa atgccactaa aagcgagcct agaggcgctt
     3841 ccctggcgat gttacttagg atgctcaatg gcatgccagg ctttcgggca gtgtcatagg
     3901 cgtcactcaa tcgatgcgct gtcgccttca cagtgtcaga cacacctgtg gctggcgact
     3961 tcacgtcttc tatcgtttcc gcgccttcat aaataaacgg cggcgggggc acatcgtcgg
     4021 tcatggcgca ccctccaatt aaatttggtc ctgcagacaa acgtcaattg atcgcgcgca
     4081 agattgttcc gcaggcggat gacagccccg ccattgggct agccggatcg cccaattcat
     4141 tccgtcagcg ggcggctggc ggtccccctt gaacggaact gcctgcgggg cggagcattg
     4201 ataggtgaaa gggagatctg aaatgggcga caaattcaat ccggcaccga tcgacaagca
     4261 tgcggccagt ccgaaagaag ctgcgaaggc cgataaagca acccacaacg aacttcagaa
     4321 gggcttgaaa gacacatttc cggcgtcaga cccagtcagt gcgacgcaac ccgcaccgtc
     4381 gaaacatgac agcgacgcca aaaaaaaggc cggctcagtc gagagcggaa acaagtctac
     4441 tcgcaaatag gatgcgacga gcttccgcgt cgttgatcgc gatggcgcaa cattgttcga
     4501 caagcttgtg acggacttca attaaacgcg ccgagggcta aaaacggctt tgacctgaac
     4561 cggaagtcac atggtcggga acatgttctc ctgtgcgcct gctatgtcgt ctgcaccaca
     4621 aacggagacg ccaaatggtg aacgaagcat gcacgggccg cgtttgttgg cgccctctta
     4681 tctagtctat cacgtcgcct cttggcactt ttcggacctc ccgctatgtc cgcttgatgt
     4741 ctgctttcag gggaagatcg gaagtcgcga ttgcgaccgc cgacttccga gtttgatgct
     4801 gtggacgggt gcgccgggag accggcaaaa ggtagacggt ggaagtccgt cacggtgaag
     4861 gtgtagcgaa ccgtaccggc cccgagtgat gcacgggttg tccgcgaggg cagacgtgaa
     4921 gcgttcacag gggagggtgc aggcgaggta ttgagcggcg taagacaagt tcggggtgct
     4981 gatgttttcc gtcccacaga aggcaacacg gccggtgtcg atatcgcgag acacatgccg
     5041 accccgcgtc gtcgtagacc ctggcatgcg cagaagcctt ttgcacggga accgggagat
     5101 ctccatcgcc gtccccgcca gtcgtggccg ggacgttgac gggaaggccg gaggccgtag
     5161 ccgacgatga gcgcgatgga gaagtcggac ctgcccgaag tagcgaggaa gcgagcgaac
     5221 aaagcggcct cggccgctgc ggagcccgtg gagcgaaggg gcggggccaa ggagaatgcg
     5281 gagctgcaaa gcacggtccg gacgcagagc cgggaggccg tgtcacgcgc gcaggcccgc
     5341 atacgagaag ccgtcaccag gaacagacag gacaagctga cggcgctcct gcatcacgtc
     5401 aacattgagg ttctgcgggc aagcttcttc ggactaaaga agactgctgc cccaggcgtc
     5461 gatgagagga cgtggatcga atacgcagag caccttgagg caaacctctt gggcctgcat
     5521 gttcgcgtcc atacaggagc gtatcgagcg ctgccgtcac gccgtaagta cataccgaag
     5581 gcggatggca gacagcggcc gctcggcatc gctgccatag aggacaagat tgtccaggct
     5641 gcggtggtga cagtcctgac gccgatctat gagtcggagt tcctgggctt cagctacggt
     5701 ttccgaccga agcgtaacca gcatcaagcg ctggatgcgc tcgcgttcgg gatcgggaag
     5761 cggcggatca actgggtact cgactgcgac gttcaatcct ttttcgacca ggttgaccag
     5821 aagtggttgg tccgcttcgt cgagcatcgg attggcgacc agcgaattat ccggctgatc
     5881 ggcaaatggc tgacggcagg cgtgctcgaa gatggacacc tgatcgagac ggaggagggg
     5941 acgccgcagg gcgcggtcat cagcccgctc ttggcgaaca tttacctcca tcatgtctat
     6001 gatcaatggg tccaccagtg gcggcagcga tgcgccaccg gtgacgtgat tgtcgtgcgc
     6061 tacgcggacg actccatcgt tggcttcgag catcggcacg aagcagagca attccttgcc
     6121 gacctcaagg cgaggctcgc tcgttttgga ctgaatcttc atcccgagaa gacgcggctc
     6181 atcgagtttg ggcggaacgc aattgcgaac cgccgcactc gtgggctcgg taagccggag
     6241 acgttcgatt tcctagggtt caagcattac tgcgcgacca gaagggatgg cagcggcttc
     6301 gtgctcggaa ggaagccaat ggccaagcgc atgcgaacca aacttcggga gatcaaggag
     6361 cagctcatgg cgacccgcca cgacgggatc gacgggcaag gcaaatggct tgcccaagtc
     6421 ctgcggggct ggatggccta ctatgctgtg ccgatgagcg gctcagcgat ctcggcattc
     6481 cggcatcata tgatcgaacg ctggcacggc gctctcatgc gccgcagcca gcggcgccga
     6541 ttaacgtgga cccgaatgaa gacgatcgct gatcgatacc tgccattccc acgcatcctg
     6601 catccgtggc ctgagaagcg gttcctcgtc accatccaag gtgggagccc ggtgcgttag
     6661 cagcgcccgc cgggttctgt gcggggggcg gagagtaatc tccgtcccta ccgcgacctc
     6721 ctccaccggc acatcggtgc catggatatg ggtgctgtta aaggctccca cgattcggag
     6781 gagcgagcca tgcagacgat tacgacaatc ggtttagaca ttgccaagtc ggttttccaa
     6841 gtccatgggg ttgatgccgg cggccaagtg gtcattcgcc gtcagttgaa gcggcgctac
     6901 gtcctggcgt tcttccagaa ggtgccacca tgccttgtcg gcatcgaagc ctgcgcctcg
     6961 tctcaccact ggtcgcgcga gctcaaagcg ctcggccata acgtgcggct gataccaccc
     7021 gcctacgtga aaccctacgt caagcggcag aagaacgacg ctgccgacgc cgaagcaatc
     7081 tgcgaagccg tcaccagagc taacatgcgg ttcgttgaga ccaagacccc cgagcagcag
     7141 agtggcatgg tgcttcatcg cacgcgtcat ctgtgtatgc gtcagcaaat ctcagtgatc
     7201 aatgcgatac gcgcccattt tgccgagttc ggaattgtcg ctgctgtcgg tcgcaaaggt
     7261 gttgaggaac tgctcaatat cgtcgctgat ccgagtgaca agcgagtgcc ggaaatagcc
     7321 cgcacttgtc tcgcagctct cgggacacaa ttgcgcaggc tcaaagagca gattctggag
     7381 ttcgaccgga tgatcagggc ctggcaccgt tccagtgaga tgagcatgag gctggatgta
     7441 gcgcccggcg tcggtccggt gctggccacc gctctggttg ctaccgttgc agacccaaac
     7501 gcctttcgat cggggcgtaa cttctcggcc tggattgggc tggttcctaa acagcattca
     7561 agtgggggca agaacaggct cggcagtatc agcaaacagg gtgaccgcta tctgcgcggc
     7621 ttgttcgtga tcggcgcgct cgccgtcatc cggtatgcca agatccatgg caccaagcat
     7681 cgaccctggc tcacggcatt gctggcgcgg aagccggcca aggtggcagc catcgcgctt
     7741 gccaacaaga tggcgcgaat ggtctgggcc atgatggcca gaggcgagcg atacaaggaa
     7801 cccgtcgcgc ttgcgcgatg agcgagatcg cgccggacaa ccggcgtgat gtgaggattg
     7861 ggagggcgaa cagcacgtaa tgcagagccg gtcgatccgg cgatcaggac aacccactcg
     7921 tgccatcgca ttgtcgaatg cggcttgttg accgggacct gatccgcgga gggcattatg
     7981 gccagcggtc atgtgaaccg cattaaaagg ccgaacacat ggctgcaccg accaatcctg
     8041 caaaacgtga agaaagctct tgccaaaccg gagccgtcca cacatggcac gtttcggaca
     8101 tgccgcaatg tccgagttga gtccgctatc gggggcaaag cggactatat gtgctcggag
     8161 cgagtatttc cgagtttgat gctgtggacg gctcctccac cggcacatcg gtgccatgga
     8221 tgtgggcgct gttaaggctc ccatgatcag aggagcgagc catgcagacg ataacgacaa
     8281 tcggtttaga catcgccaag tcggttttcc aggttcacgg cgttgatgct ggcggccaag
     8341 tgattgttcg tcggcaattg aagcgtcgct acgtcctgac gttcttccag aagctatcgc
     8401 cctgcctggt cggtatcgaa gcctgcgcct cggcgcacca ctggtcacgc gagctccagg
     8461 cacttggtca cctcgtccgg ctgatgccgc cggcctacgt gaagccctac gtcaagcggc
     8521 agaagaatga cgcgactgac gcggaggcta tttgcgaggc agtcagcagg gccaacatgc
     8581 ggttcgtgcc gaccaagacc cccgagcagc aaagcggcct ggtgttgcac cgcacgcgcc
     8641 atctgttcat ccgtcagcag acctcggcga tcaatgcgat ccgggctcat cttgccgagt
     8701 tcgggatcgt tgcgccggtt gggcgtcacg gcgtcgagga gctgctcact gtcgtcgccg
     8761 atccgaacga cagacgggtt ccagagatag ctcgtgcgtg tctctcggca ctcggggctc
     8821 aactgcgaag gatcaaggag cagatactcg agttcgaccg gctgatcagg gcctggcacc
     8881 gatccaacga gatgagcatg aggctcgaag aagcccccgg cgtcggtccg gtgctggcta
     8941 ctgctttggt cgctgccgtt gctgatccaa agtccttccg atcagggcgc aacttctcgg
     9001 cctggatcgg tattgtgccg aaacagcact cgagcggagg caagaacagg ctcggcaata
     9061 taagcaagca aggtgaccgc tatctgcgcg gcctgttcgt ggccggcgcg ctttcggtca
     9121 tccgctacgc caagatccat ggcaccaaac atcggccctg gcttacggcc ttgctggcgc
     9181 ggcggccgac caaggttgcg gctatcgcgc ttgccaacaa aatcgcgcga atggtctggg
     9241 ccatgatggc caggggcgag cgatacaa
//
LOCUS       DATMGX010000553         2663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_290426, whole genome shotgun sequence.
ACCESSION   DATMGX010000553 DATMGX010000000
VERSION     DATMGX010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290426"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VNR65_09010"
     CDS             <1..503
                     /locus_tag="VNR65_09010"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HWJ18850.1"
                     /translation="ALAERFTRAHDDYSKIMVKALADRLAEAFAEHLHERVRREFWGY
                     AKDETLNSTDLLAEKYKGIRPAPGYPAQPDHTEKGPLFEMLNAVRATGIKLTESYAMM
                     PAAAVSGFYFSHPESHYFGVGKIERDQVEDYAKRKDWPVSEAERWLAPILNYDRETKE
                     PESAAA"
     gene            complement(608..1984)
                     /locus_tag="VNR65_09015"
     CDS             complement(608..1984)
                     /locus_tag="VNR65_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWJ18851.1"
                     /translation="MKGKKTKIRIEGNHGVWHSWLGRGDIKWDNAEYYVIAYRERSLV
                     CHYSIRNYSANEVWFLPWENTDPEECDLGQLVPSRASKVDEDCLLAWAQLKDSTDLKL
                     VENFRLRYGPSNIDCKALAAKRIAELVAAQTPDVDKGKTPVSVSQPPESEAAVVKPRP
                     TRSDQTAKAVMIPISPRPGKAWLGVQVQNVDSKMATAIGMPSARGALVAKILSDGPAG
                     RSELKEGDVILAANGRAVGDSSDFAAIVQESSPGDKVSFRIYRQQKEMDLVVTLESAN
                     LPADLARQAAINPRAEQEASAIAKFNLRRNRDIYGHDIPFPQGKIAGVDIKECAAQCD
                     KLQACVAFSYDRWNSLCLLKNEITTSMLDVRSTIAVKKPGELPNISKKKAEILAIHKV
                     RFRGDMISTKSVPDLDACRTSCDNDMSCIGFTFGKGDVTNNCMKYKDLQGYDRDAQME
                     SGYKYQSP"
     gene            complement(2218..>2663)
                     /locus_tag="VNR65_09020"
     CDS             complement(2218..>2663)
                     /locus_tag="VNR65_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18852.1"
                     /translation="IGHGYKPLRLLVIMAVVGVMFGFYYKLAAERGIFAPRDAQVFTQ
                     ATFDKCRGRPGGWTTCAEVEPTFAEYSQFNPWVYSFNVLLPVVDLYQEKYWVPMRKEV
                     PFEAGGYAFVVPGWGTNALVLTELVFGWVASLLAVAAFSGLVKTD"
BASE COUNT          583 a          762 c          720 g          598 t
ORIGIN      
        1 aagcccttgc cgagcgcttc acgcgcgccc acgacgacta ctcaaagatc atggtaaagg
       61 cgctggcgga ccgccttgcc gaagctttcg ctgagcattt gcacgagcgc gtgcgccgcg
      121 agttctgggg ttatgccaag gacgagacac tgaactcgac tgatctcctc gccgagaaat
      181 ataagggcat tcgccctgcc cccggctatc cagcgcaacc cgatcacacg gagaagggac
      241 cgctgttcga gatgctcaat gcggtacggg caacgggcat caagctgacg gagagctacg
      301 cgatgatgcc tgcagccgcg gtttcgggct tctatttctc tcacccggaa agccactatt
      361 tcggcgtcgg caagatcgag cgcgaccagg tcgaagacta cgcgaagcgc aaggactggc
      421 cagtctcgga ggccgaacgc tggcttgctc ccatcctcaa ttatgatcgg gagacaaaag
      481 aacctgaaag cgcggcagct tagagcgcat tcaggaaaag tgtgtgcggt tttccgcccg
      541 gaaagcgtga tggaatataa ggtcagagcg catttggcga ctcgagaatg cgctctggtc
      601 cgcccggcta gggagactgg tacttgtagc cgctctccat ctgggcgtcc cggtcgtagc
      661 cttgcagatc tttgtacttc atgcagttgt tggtcacgtc gcctttcccg aaggtaaagc
      721 cgatgcaact catgtcgtta tcacagctgg tgcggcacgc gtccaaatcc gggacgctct
      781 tggtgctaat catgtcgccc ctgaaccgaa ctttatggat ggcaagtatt tcagctttct
      841 tttttgatat attgggaagc tctcctggtt ttttaacggc gatcgtcgat cgcacgtcca
      901 gcatcgacgt cgtgatttca ttcttcagta agcataagct gttccatcga tcgtagctga
      961 aagcaacgca agcctgcaac ttatcgcatt gcgcagcaca ttccttgatg tcgacgcctg
     1021 cgatttttcc ttgcggaaat ggaatatcgt gtccgtaaat gtctcgattg cgccggagat
     1081 tgaatttggc gatagcagaa gcctcttgct cagctcttgg attgatcgcc gcttgtcttg
     1141 ccaaatccgc aggcaaattg gcggactcta gcgtcacgac caaatccatt tccttttgtt
     1201 gtctgtagat gcgaaagctg accttgtctc ctgggctgct ctcttggacg atcgcggcaa
     1261 agtcgctgga atcgccgacc gctctaccgt ttgctgcaag gatgacatcg ccttctttca
     1321 actcggaacg ccctgcagga ccatccgaca gtattttagc aaccagggcg ccccgcgcgg
     1381 acggcatgcc gatcgcggtc gccatctttg agtccacatt ttgcacttgc accccaagcc
     1441 acgccttccc aggcctgggg gagataggaa tcatgactgc cttagctgtc tgatcagacc
     1501 gggttggacg cggtttcacg acggcagctt cgctttcggg cggctgcgaa acggacactg
     1561 gtgttttgcc cttgtcgacg tctggcgtct gggcggccac taattccgcg atgcgcttgg
     1621 ccgcaagcgc cttgcaatcg atgttcgagg gtccgtaccg cagccgaaag ttttcgacaa
     1681 gcttcagatc ggtcgagtcc ttgagttgtg cccaggcaag caagcaatcc tcgtcaacct
     1741 tgcttgcacg agacggaacg agttgcccga gatcgcactc ttcagggtca gtattctccc
     1801 agggaagaaa ccagacttca ttcgcagaat agttcctgat ggaatagtga caaactaaag
     1861 accgttcgcg ataggcgata acataatatt ccgcattgtc ccactttata tccccacggc
     1921 ccagccatga atgccagaca ccgtggttcc cctctattct tatttttgtt ttctttcctt
     1981 tcacatccca ttccccggcc aggagcagga tttcatcgac ggtggtttgc gccgaacttg
     2041 gcgacagcca agggctcagc atgacgagcg caaggaaaag ccaaagtcgc aggacccaac
     2101 tcattttgct gcttcgatgc ctctctacgt actcgtttga ttatgctagc gaacccccac
     2161 agtgtgcaag agagaggaat gcgaactctc cgtgattgtc attcactaaa acgcccccta
     2221 atccgtcttg acaaggccgg agaaagcggc aacggccaga aggctcgcga cccagccgaa
     2281 gacaagctcg gtcagcacga gcgcgttggt gccccagcct ggaaccacaa aagcgtagcc
     2341 gccagcctcg aatgggactt cctttcgcat cggcacccaa tatttttcct gatagaggtc
     2401 gacgacggga agcagcacat tgaaggaata gacccagggg ttgaactggg aatattccgc
     2461 gaaggtgggc tcaacttccg cgcacgtcgt ccaaccgccg gggcggcctc ggcatttgtc
     2521 gaaggtggct tgcgtaaaca cctgcgcatc gcgcggggcg aagatgcctc tctcggcagc
     2581 gagcttgtag taaaacccga acatcacccc gacaacggcc atgatgacga ggaggcggag
     2641 cggcttgtat ccgtggccaa tca
//
LOCUS       DATMGX010000554         4184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_363593, whole genome shotgun sequence.
ACCESSION   DATMGX010000554 DATMGX010000000
VERSION     DATMGX010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363593"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..459)
                     /locus_tag="VNR65_09025"
     CDS             complement(274..459)
                     /locus_tag="VNR65_09025"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HWJ18853.1"
                     /translation="MRPKTQGAVVVFSWIAYQSREDRDRVVGAVMSDSRMGSDPQATP
                     FDGKRMFWGGFKVLLAM"
     gene            603..899
                     /locus_tag="VNR65_09030"
     CDS             603..899
                     /locus_tag="VNR65_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWJ18854.1"
                     /translation="MAKMTKNQLIDAIAEGTQVSKADVKAVIEQMATVGYKELNESGE
                     FVIPGFAKMSVVNKPATEARSGVNPFTKEPMEFAAKPASKSVKASPLKVVKDAV"
     gene            1297..2193
                     /locus_tag="VNR65_09035"
     CDS             1297..2193
                     /locus_tag="VNR65_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18855.1"
                     /translation="MADDAIASIIALHQPKRAKTAAQRARAYRPRKRQKAKAAPSPEA
                     EPRSSELLIPAEFSSANPAFAELPVTPSHRVASILLAAAAFALAGVGVTTNGWFARSL
                     GSSDIAGWLFLAIGVATDLAALVLPSCAAALWHTGQRATSLAGWAAWMMTFAFAVTAG
                     IGFASVNITDVTAARAARLTPAVTAAQSALSDAMSARDRECLHGVGKFCREREATVAE
                     RRQILDTAMASVGYTADPQNDAAIKLVAWVTHGVLRPSGDDFAMLRLVLLAMLPQIGG
                     ILLMVSRSAMRPSASALQISRP"
     gene            complement(2505..2681)
                     /locus_tag="VNR65_09040"
     CDS             complement(2505..2681)
                     /locus_tag="VNR65_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18856.1"
                     /translation="MKPMPDADAHRRLGKVEAEKARKLPPGRDKEVHLQNARDHESSA
                     HAKDWRDSNLRAPE"
     gene            complement(2976..3131)
                     /locus_tag="VNR65_09045"
     CDS             complement(2976..3131)
                     /locus_tag="VNR65_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18857.1"
                     /translation="MCEKCVEIDKTIERYRRILLSISDQLTVDRTRQLIADLQAKKAA
                     LHPEQAG"
     gene            3603..3830
                     /locus_tag="VNR65_09050"
     CDS             3603..3830
                     /locus_tag="VNR65_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18858.1"
                     /translation="MKQEEAKQLIIREWDRWVRTQPIDSGGATGRDSLKFFLELQDAR
                     SPLLDFQTRGRDKWQVVHAWLLSARRLKSAS"
     gene            complement(3912..>4184)
                     /locus_tag="VNR65_09055"
     CDS             complement(3912..>4184)
                     /locus_tag="VNR65_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18859.1"
                     /translation="IVWMSLYFSAAVWASLALCVCYSLEDHLPRYRPEATVASWSRDA
                     PAAAGRQHAAMARQAYDSHEAGLGTLGDHDEVSTYLRDAGWRVPFR"
BASE COUNT          820 a         1252 c         1214 g          898 t
ORIGIN      
        1 gccgcgtcca ccgcatcccg ccccaacgtt cgtgacgatg gccaacgccc ctctcccggg
       61 acaggatggc cgggatatga aagtgatttg gatcgggtga gaacagaaat atttttgcaa
      121 agggagctgg acaggccact cgcaaaacag ccacctgatt tgcccgtcgg gttgattgtg
      181 tctccggtca agtgccgccg tccggtcttg gcgcaaagcg gcctttaagt aaaaatcgcc
      241 gccgcaagta gacgccgtcc tgtgcggtag ttttcacatt gcaaggagga ccttgaaccc
      301 gccccaaaac atccgcttgc cgtcgaacgg cgttgcctgc ggatccgatc ccatgcgtga
      361 gtcgctcatg acagcgccaa cgacgcgatc gcgatcctct cgcgactggt aggcgatcca
      421 cgagaacacc acgactgctc cttgcgtttt gggcctcaat aggttttaag cccggtgact
      481 tcatgaccat aggcatgaac gagaagcgaa ccgcgtggct ttgccgcgtt ttgaacagag
      541 tgacgcaccc cgcgcagctc gcgcgcctta gattattctg atcctcccta aaggaatcca
      601 tcatggccaa gatgaccaag aatcagttga ttgatgcaat tgcagaggga acgcaggttt
      661 cgaaagcaga cgtgaaggcc gtcatcgagc agatggcgac tgtagggtat aaggaactga
      721 atgaatccgg cgagttcgtc attcctggct tcgccaaaat gtcggtcgtg aacaagcctg
      781 ctactgaagc ccgcagcggg gtaaatcctt tcacgaaaga gcctatggag ttcgcggcca
      841 agccagccag caagtcggtc aaggcgtcac cgctgaaggt tgttaaagac gctgtttgac
      901 gcggacgggt tgcttgagac gtgttcgttc gtctgaactg tgggtaattc catcacacag
      961 acaggcacct ccacccgtac gctatgcgcc ccgagatgga atctctggag ggcccaggac
     1021 gccgaggttg gctaaatgcc agtacccgga gctttggctg acagtttgaa actgattcta
     1081 ttttccgcca tggccgggct tgtcccggcc atccgcgcct ttcttgattc cgccggcttt
     1141 tatccccatg aagctcgctc ctgcgatttg attccgtggg tgaacgatgt caccgttgtc
     1201 cggtcaacag cacacgcaca tgaatcgagt cggcgcgacg tggtgtttgc accacgctgc
     1261 cgcgcctaac caccgcaacc gaatggagcg gttccaatgg ctgacgacgc tattgcttcg
     1321 attatcgccc tgcatcaacc caaaagggcc aagaccgctg cccaacgcgc ccgcgcgtac
     1381 cggccgcgca aacggcaaaa ggcaaaggcg gctccctcgc cagaagccga acctcgatct
     1441 tccgaattac tgataccggc agagttctcg tcggcgaatc cggcatttgc cgaattgccc
     1501 gtcacgccgt cacaccgcgt cgcctccatt ctcctggcgg ccgcggcgtt cgcgctcgct
     1561 ggagtcggcg tcaccaccaa cggctggttc gcacgctcgc ttgggtcgag cgacatcgct
     1621 ggatggctgt tcctggccat cggcgtagct accgatctcg ccgcgctggt cttgccgtca
     1681 tgtgcagcag cgctttggca caccggccag cgcgctacct ctcttgcagg atgggccgca
     1741 tggatgatga cctttgcctt tgcggtcacc gccggcatcg gcttcgcgtc ggtcaacatc
     1801 accgatgtta cggctgcgcg ggcggcgcgc ctaacgcccg ccgtgacggc ggcccaatcg
     1861 gcgttgagtg acgccatgtc tgcgagggac cgggagtgct tgcacggtgt tggcaaattc
     1921 tgcagggaac gcgaagcgac tgtcgccgaa cgtcgccaga tcctcgacac cgctatggca
     1981 tcggtcgggt acacggcgga cccccaaaac gacgccgcga tcaagctcgt cgcatgggtc
     2041 acccatggcg tgcttcggcc atccggcgac gacttcgcca tgcttcgcct tgtcctgctg
     2101 gcgatgctgc cgcagatcgg tgggatattg ttgatggtca gccggtcagc aatgcgacct
     2161 agcgcgagtg cgcttcagat aagccgcccc tgatccgtat aattgtccga gacgaggcga
     2221 ctcaaccgct gcggtccata gtctgatgcg ctcttactgg aagaccagaa tatgccgaac
     2281 tggcttgtgc cacctatctg cccagccgcc ctcctcgggt tcattggctt tgcatttcgt
     2341 caggggacga aggtgccacc ggatcggaat aaccgcaact ttggaccgag ccagaatgat
     2401 ggccaagtcg atgggtcgca tggtggcttc gacggtcata cttgaaacca gcacccttaa
     2461 gactgacctg cagccgatcg cgtgcgatca cgcaagccat tggcttactc cggcgcgcga
     2521 agattcgaat cccgccagtc tttcgcgtgc gcgctggatt cgtggtcccg ggcgttttgc
     2581 aaatgaactt ctttgtctct tccgggtggc agcttgcgcg ctttctctgc ttcgactttc
     2641 ccaaggcggc ggtgcgcgtc ggcgtcgggc attggcttca tgacacacct ccgatttttt
     2701 cagatcggat aatgcgtatt ttttgaatcc gttcctgtgt cgggaaccgg gcgctcaggg
     2761 cccgaattcc ttcggctttc tgaccccgcg tgccggggaa ttctcgcgat cgctgagggc
     2821 acgaccgcca cgcaaccggt ttcgccgtgt tctttcaaat cgaataatgc gtcgtgctgg
     2881 cgttcgttcc tgtgtcggaa accggacgat ttacggttgc gaaaattcag ttggaaagtc
     2941 ctgaaaaatg tcctggaggc gcaaggcgcg gcgcctcagc ccgcctgctc cgggtgcagc
     3001 gcagcctttt tggcctgcaa atcggcaatc aattgcctgg tcctgtcgac cgtgagctga
     3061 tcgcttatcg agagcaatat tcttcgatac cgctcaatgg tcttgtcgat ctcgacgcac
     3121 ttctcgcaca tggcccgccg ccccctgatc aggcggaagc aaccaggcca cctgttattt
     3181 catgacggcg gcgacgcgat tttgtttcat ggattcgtcg agcaagggca cacattagag
     3241 aaaatgtaag ttgaggagag accagcaggc agtcgcgtgg cgccgtcgtg tgtcgcatcg
     3301 gcaacaatga catgattttt ccgacggaac ggattttttt gctccacagc gccccgctgt
     3361 gccgcataaa gtgaacgtcg gggcggctgg gtcatgatcg gcggaggaac ggcgcgttcg
     3421 tagctgattt tgagtactct atcagtctac agagagaccg cctttcgggg cggtcttttt
     3481 gtttggcggt tccccataat cttcgcaaga tatgcccgcc ttttctgatc ggaaacaacg
     3541 gtttcgtaat cattcaaccg ttacggcaga tccggcgctc tatgccgtcc ggtccggatt
     3601 gaatgaaaca ggaagaagcg aagcagctga ttatccggga gtgggaccga tgggtccgga
     3661 cgcagcccat cgattccggt ggggcgaccg gccgggattc gctgaaattc tttctggagc
     3721 tgcaggatgc gcgatccccg ctgctggatt ttcaaacgag gggccgggac aagtggcaag
     3781 tcgtccacgc ctggctgctc agcgcgcggc gcctcaaatc ggcttcgtga ggaccgattt
     3841 cgtccccgcg tcgcccgact cgcaatccgg caaacgggaa aatccgccgc ttgatcgttg
     3901 ggatatcgat ctcatcgaaa tgggactcgc catccggcgt cgcgtagata cgtagacacc
     3961 tcatcgtggt ctcctaaagt accgaggccg gcctcatggc tatcgtaggc ctggcgcgcc
     4021 attgccgcat gctgcctgcc ggccgccgcc ggcgcgtccc ttgaccagct tgcaaccgtt
     4081 gcttcgggac gatagcgcgg cagatgatcc tcgagcgagt agcaaacaca cagtgcgagg
     4141 ctggcccaga ccgccgcact gaaatagagc gacatccaga cgat
//
LOCUS       DATMGX010000555         3894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_95853, whole genome shotgun sequence.
ACCESSION   DATMGX010000555 DATMGX010000000
VERSION     DATMGX010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95853"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1717..1959)
                     /locus_tag="VNR65_09060"
     CDS             complement(1717..1959)
                     /locus_tag="VNR65_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18860.1"
                     /translation="MAAPEIGAIDQQARTPAARISANVIFWLVSLVMAHDRTDQDGKE
                     AAGYGAFAAWAHYDARRGRGCVRTVVGILWGAARLR"
     gene            2552..2731
                     /locus_tag="VNR65_09065"
     CDS             2552..2731
                     /locus_tag="VNR65_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18861.1"
                     /translation="MPHLSANRFYLAATVSALILGTLVSLGTILIRNGDPADCRITSS
                     AVSGAAFVVETSLMH"
     gene            complement(2859..2993)
                     /locus_tag="VNR65_09070"
     CDS             complement(2859..2993)
                     /locus_tag="VNR65_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18862.1"
                     /translation="MIKGAAIAAVVLFIAVQVDQHYDNGFYTDGVLAMLHQIRHSFVW
                     "
     gene            complement(3274..>3894)
                     /locus_tag="VNR65_09075"
     CDS             complement(3274..>3894)
                     /locus_tag="VNR65_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWJ18863.1"
                     /translation="AQAGKLINLSDAERATSEIINSIVLNGLGAWLDDVRRYHEGTYQ
                     HCLLVTGVAVSFALDIGFSSLDVKRLGMAATLHDIGKARIPLSILDKPGRLDPAEEEI
                     MRRHPVIGCELLKNTSGISPEILDGVRHHHEYLDGSGYPDALTAPEISDLVRLLTISD
                     IFAALIESRPYSPPISRQDAYKILCGMDGRLERPLVKAFRNVALVD"
BASE COUNT          830 a         1106 c         1087 g          871 t
ORIGIN      
        1 atttcactgg gctggaaatg cgctttctat tggctgtgat gttagcgttt ttcgttggct
       61 caaccctcgc gtttccagcc gagggaaaga ccgtgagcgg cgcaccggcc aaagtgacgc
      121 gattgaccca gactgtgtga aacgatagct gcaatgcgcc tttggcgcag cacatctcag
      181 aatgctgcgt aggcttgcct aaatgcaatg gcacgcgcta acgatctcgg acgtgtccga
      241 acggcatgtc catcattgcc gctccggacg atccactggc cgttctcgtg gcccacgatc
      301 tttcctacgt ggtgacgcca gacgacgata gcaccgactg ccggaccacc cgcgtttgat
      361 ccgtaatgag cccatgaacg cgcgagatta aaggacggcc cgggatcgcg gcccactagc
      421 tggcgcatgt accatccgca ccatgcgctg ggccgacctg catagtgatg tcgacccgtg
      481 tgatggtggt gagacgatcg agcctctgca ctcgatccaa ccaaacaaag tactgccgtt
      541 gcagccatga gagtacgcat catggatgtt ccttactggg cgagagatcg cactgcgcga
      601 tcaagaagcg gctggcgtca gggacaatta aattccaggg cggcgcgttt aaatgagcgg
      661 aattaaattc tagcggcggt ttataagggg ttatacatgg caaaaatacg gcactaagtg
      721 cttcctcagg ctccattttc ataccggcgc actggcgccg gtttaaataa caaaagattt
      781 ctgttattta accggtaatg ttccagcgga ggtttaactc acgggaaacg ctatcatctc
      841 catcgcggca tgatctagcc tgcgcgctct tagacctcac cggaactccc ggatgacaca
      901 cccatcgaaa acgtccgatt ttcgaccagg atccggaacg cggtaatagc cgcgggttgg
      961 aaaaccgtcg gggaaattcg agaggcttcc gacgcaacac ttttgggcct gccggatctc
     1021 ggaaagggct ctgtcgccca tattcgagaa acgctgggcc tgccgtcgac ggatggcgtg
     1081 cggccggggc cgaaggcgaa gggaaaatga aacgctacgg cgtgaacgct acagaccgac
     1141 ggcaaccgtg agcttcaaga gtccgctggc ggcactctga ggattttccc tgcaggccct
     1201 ggtagtgttc gcgcttctgg attggctcat tcatcgggct gaaggcggcc cgaaatgggc
     1261 ccgcgccgcc gcccggtggc cgcgccggtg cgagttttcg aacactctct acaagctgcc
     1321 gaagctgatt ttctctgttg ccccttagcg actaagtttt tgttcctgct tcggaaccgc
     1381 acattttaat ttacgccctt ttcaagatgg ctttaacttt gttcaccggg ctggggtgca
     1441 ttttataaat actcaatttt tgaacccggt acgccgcaga gcgaaaatgc ctgcggcttt
     1501 tttccggggg ctttttgaaa tgctcttcga tgtcatcacc actcttattc tgttttatgg
     1561 ctttagcgtc cttcctcggc tgatctcgcg acggcgcggc gacgtcctgt actacgtttc
     1621 aaggccagac ccccttcggc ggagcgcact gccgcagcat ccggttgtgg cgttggcaga
     1681 cggcccgcgc cgccgcgggg tgaccgcctc catgcgttat ctcaggcggg ctgcccccca
     1741 caaaatgccc accaccgttc gaacgcatcc acggccgcgc cgcgcatcgt agtgggccca
     1801 cgcggcgaag gccccatatc cagccgcttc cttcccgtcc tggtcggtgc gatcatgggc
     1861 catgaccaag ctcacaagcc agaaaataac gttcgccgag atgcgcgccg ccggcgtgcg
     1921 cgcctgctga tctattgctc cgatttccgg tgcagccatt ggaccgcgat cagcggcgac
     1981 cggtgcccga cgaagccctc aagatcgtcg cgcgtggcgt cgataaggaa gatcgggcgg
     2041 cggcttgacg cacaagtcga gtaactgaag cgttttgcga ctcagcaaaa cgtggtctcc
     2101 ttttgcaaac tgcacgttga ggcaggagcc ggggagatga ctcagattgc ttttgcggtt
     2161 ttaatagtgc tgggcttgtc gctaccgagc tactttggat tggcggcatg gcggtcgtca
     2221 ggtgatgatc gaggagagat caccgggacc atttttccac gaggtcggcg actctttagc
     2281 gcaacagtcc gactttttcg ttcttagggt atcgttttag cctatcgctg ctacatgagc
     2341 gaagtagtct tgggctgcgg agcgcatttt aatccgcaag cgcgttagag actgatggac
     2401 ccatttgaca ataagccgtt cctgcccgtg gacgtgatga tcacgatcgc gatctttggt
     2461 ggtgctgcct ccagtgaagc gaatgcaacg ccactaattc gcccgactca gaaccccacc
     2521 tctagtgggg tggtaacgtg gggagttggt aatgccgcat ttgtccgcaa acaggttcta
     2581 cctagccgca actgtcagcg cgcttattct cggcacgctg gtatcgcttg gcacaatcct
     2641 tattcggaac ggcgacccgg ctgattgccg cattacgtca agcgctgtat cgggtgcggc
     2701 gttcgtggtc gagactagcc tgatgcacta actgccagcg ataggctgct ccacggacga
     2761 ggtgtcaccg gagggcaaaa tctatcccgc actatgtaaa aagtgcggcg gcaaggggca
     2821 agtagagccc gccgactgaa ggatacctgc tgcggttttt accagacgaa agagtgccga
     2881 atctgatgca acatggctag aacaccatcc gtgtaaaagc cgttgtcgta gtgctggtca
     2941 acctgaacag caatgaaaag caccactgcc gcaattgctg cgcccttgat catggcaggt
     3001 taccccccaa tgacggcctc agggtcctga attagaaagt tgaacaaccc gcaggccaaa
     3061 aaaacggcaa tacaatcgcg gcaagaatga cagcacgtcg taaatgaggt cttaaaggct
     3121 accaaaccgc cttaggacct cagcgggttc gtaaaagccg ctctcgggat atccgatgcg
     3181 cgagaatgcc cgtgcgccaa tgtatccggg cgctagccat ccaccgagcg cgttcgatag
     3241 cggcttcatt tgtctcgctt ccgcagattt gcttcagtcc acaagcgcta cgttccggaa
     3301 cgccttcaca agcggtcgtt ccaaccttcc gtccataccg cacaggattt tgtaggcatc
     3361 ttggcgagat attggcgggc tgtatggcct cgactcgata agcgcggcga agatatccga
     3421 gatcgtcagc aatcttacca gatcggaaat ctccggtgcc gtcagtgcgt cgggataacc
     3481 agaaccgtcc agatattcgt ggtggtgcct cacgccgtca agaatctccg gactgatgcc
     3541 tgaggtattc ttcaataact cgcatccgat gaccggatgg cgtctcatga tctcttcttc
     3601 agctgggtcg aggcgtcccg gcttgtcgag aatcgataac ggaatacgag ccttcccgat
     3661 atcatgaagg gttgccgcca ttccgagtcg ctttacatcc agactggaaa acccgatgtc
     3721 caatgcgaag ctgactgcca ctcccgttac cagcaggcaa tgttggtacg ttccctcatg
     3781 atagcggcgc acatcgtcga gccacgcgcc aagcccgttt agcacgatgc tattgataat
     3841 ttctgatgtg gcgcgctcgg cgtctgaaag gtttatcagt ttgccggcct gcgc
//
LOCUS       DATMGX010000556         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_262160, whole genome shotgun sequence.
ACCESSION   DATMGX010000556 DATMGX010000000
VERSION     DATMGX010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262160"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VNR65_09080"
     CDS             complement(<1..398)
                     /locus_tag="VNR65_09080"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ18864.1"
                     /translation="MNSPNIFDRRLIQANLTRAAHALPGTDFLLRHAAEELVGRLEGI
                     SRTFDLAADASAYHGVVAGLLNKRFPGTKTICLSPVMALAEACPAPRAVATEEALPLK
                     AGSLDLVTSALSLHLVNDLPGALIQIRRALR"
     gene            457..1239
                     /locus_tag="VNR65_09085"
     CDS             457..1239
                     /locus_tag="VNR65_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HWJ18865.1"
                     /translation="MFQRVAQFAADGLRYSTDLILPAVCVSCSTLITRHNLLCPRCWS
                     GLNLITAPFCDRLGIPLPAYEGTETHISMQALANPPIFLRARAAAHYGGVIRRLIVRF
                     KFEDKHEPLPLFIKLMREAGRGLLADADILVPVPLHRLRLLQRRFNQAALLAKGLGRV
                     SGVPACVTSLQRTKRTRAQVGLAQDARQHNVADAFGVSRSGRNAVRDKRVLLIDDVMT
                     TGATANACATALLGAGARSVDVLAIALVAHGIEEPLENEGEL"
     gene            1316..1570
                     /gene="grxC"
                     /locus_tag="VNR65_09090"
     CDS             1316..1570
                     /gene="grxC"
                     /locus_tag="VNR65_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HWJ18866.1"
                     /translation="MPPVTIYTTPSCPYCRRAKNLLTRKGVLFEEIDASDPSIREAMI
                     ERAGGSYTVPQVFIGSTHIGGCDDLYALDAKGELDPLLQS"
     gene            1609..>2185
                     /locus_tag="VNR65_09095"
     CDS             1609..>2185
                     /locus_tag="VNR65_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWJ18867.1"
                     /translation="MSDKKDHFRVALVQMRSGRDVRDNLLEAEAFIRDAAGKGAQFIQ
                     TPENTLLMETETARLFEKIAPEEATEAIPHFSALAKSLGIWLHIGSIAIKVDEKRAIN
                     RAYLFAPDGQIALRYDKIHMFDVNLPSGEIYRESAIYVPGKTAYVVQLPWGGLGVATC
                     YDIRFPEQYKALAKAGAKFLTAPSAFTKVTGE"
BASE COUNT          473 a          622 c          645 g          445 t
ORIGIN      
        1 cggagcgcgc gccgtatctg aatgagcgcg cccggcagat cgttgacgag gtgaagcgac
       61 agcgcggatg tcacgaggtc gagcgaaccg gccttcaaag ggagtgcttc ctccgtagcc
      121 acggcgcgcg gggctggaca agcctcggcg agcgccatca ccgggctcaa acaaatcgtt
      181 tttgtgcccg gaaagcgctt gttgaggagc ccggcgacaa cgccatgata ggcgctggcg
      241 tcggcggcga ggtcgaaggt ccgactgatg ccttcgagcc ggccaacaag ctcctcggcg
      301 gcatggcgca gcagaaaatc ggttcctggg agcgcgtgcg cggctcgcgt gagattggcc
      361 tggataagcc ggcgatcgaa aatattgggg ctgttcaaag gctttacccg gatctatggt
      421 gcattttaga aaaagtcatt cctatagaaa ttgcaaatgt tccagcgtgt agcccaattc
      481 gccgccgacg gcctgagata ctccaccgat ctgatcctcc cggccgtgtg cgtctcctgc
      541 tccacgctca ttacccgcca caatcttctc tgtccccgat gctggagcgg gctcaatctc
      601 atcacggcgc ctttttgtga caggctcggc attccccttc ccgcttatga agggacggaa
      661 acgcatatct caatgcaagc cctggcgaac cctcccatat ttttgcgggc gagggccgct
      721 gcgcattatg gaggcgtcat ccgccgtctt atcgtgcgct tcaagttcga ggacaagcat
      781 gagccgctgc ctctgttcat aaagctgatg cgggaggccg gccgcggcct tctggctgat
      841 gccgatatcc ttgtccccgt tccgcttcat cgccttcggc tcttgcagcg gcgcttcaac
      901 caagccgccc tgctggccaa aggcttaggc cgggtgtctg gtgtccccgc ttgcgtcact
      961 tcccttcaga ggacgaagcg aaccagggcg caagttggcc tcgctcaaga tgcacgccaa
     1021 cacaatgtgg cggatgcctt tggagtgagc cgaagcggtc gaaacgcggt gcgcgataag
     1081 cgcgttttgc tgatagatga cgtcatgacc accggcgcga cggcgaatgc ttgtgcgaca
     1141 gctctccttg gtgcaggagc acgcagcgtg gatgtgttgg caatcgctct tgttgcgcat
     1201 ggcatcgagg agccgttgga gaatgaggga gagctataag gccgcatcag ccgaagctgg
     1261 actgagccgc tgttagaggc catgtctctg cgcgcgaatg ctgaaaggaa cactgatgcc
     1321 gcccgtgacc atctatacaa cgccgtcctg cccttattgc cgtcgcgcaa agaacctctt
     1381 aacgcgcaaa ggtgttttat tcgaagagat tgatgcgagc gacccctcca ttcgcgaagc
     1441 catgatagaa cgcgcgggcg gcagttatac tgttccccaa gtctttatag ggtccacgca
     1501 tattggaggt tgcgacgacc tttatgctct cgatgcgaaa ggagaacttg atcccctcct
     1561 gcaaagctga gcaaaatagc aggcaacgat aagggaggca gataaagcat gtcggacaag
     1621 aaagatcatt ttcgagtggc cctggttcaa atgcgctcgg ggagagacgt tcgagataat
     1681 cttttggaag ccgaggcctt tatccgcgat gctgccggaa aaggggccca gttcatacag
     1741 acccctgaaa acacgctttt gatggagacc gaaacggcgc gtctgtttga gaaaatcgcg
     1801 ccggaggaag cgaccgaagc aatcccgcat ttctcggccc tcgcaaagag cttgggcatc
     1861 tggctccata tcggttccat cgccatcaag gtcgacgaaa agcgagcgat aaatcgagcg
     1921 tatctctttg cccccgacgg gcagatcgcg ctgcggtatg acaagattca catgttcgac
     1981 gtgaatctgc cgagcggcga aatctaccgg gaatccgcaa tatatgttcc aggcaagacc
     2041 gcctatgtgg tgcaattgcc atggggaggc ctgggcgtcg cgacctgcta tgacatccgc
     2101 ttcccggagc aatacaaggc gctcgcgaaa gcaggagcga aattcctcac cgcgccttcg
     2161 gccttcacga aggtcacggg cgaaa
//
LOCUS       DATMGX010000557         6039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_193465, whole genome shotgun sequence.
ACCESSION   DATMGX010000557 DATMGX010000000
VERSION     DATMGX010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193465"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VNR65_09100"
     CDS             complement(<1..300)
                     /locus_tag="VNR65_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18868.1"
                     /translation="MIMSNGLTNATRAFIRRRLVPMLVAVPLAAVAYGAQAQTPGAWK
                     MAAPMPRPMGEIVAATANGKIYVLAGLDDVDHTPYGTVWEYDPAVNKWNVKKQMPG"
     gene            498..1169
                     /locus_tag="VNR65_09105"
     CDS             498..1169
                     /locus_tag="VNR65_09105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ18869.1"
                     /translation="MTSTLNRYQRIAPVYDLLDFPFEYGRYRKIRPLLFAGLSGLILD
                     AGVGTGRNFPFYPRDSKVVGIDLSPAMLARATRRRPSAVAEVELRQMDVTHLDFPDRF
                     FDAAVATFLFCVLPDELQVAALKEIGRVVKPGGSIRLLEYTRPRGWLRRAVTRIWEPW
                     IGWAYGASFDRRTEERIPEAGLRLCETRFVVDDLIKMISGCTVRNCRDSSPDATSIPP
                     GQHLN"
     gene            complement(2194..3489)
                     /locus_tag="VNR65_09110"
     CDS             complement(2194..3489)
                     /locus_tag="VNR65_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HWJ18870.1"
                     /translation="MPTLSKHPETIALHGGNYRADPATTAVAVPIYQTTSYQFNSTEH
                     AANLFALKELGNIYTRIMNPTQAVLEERMAAFEGGVAALAVSSGQAASLFAVQNICHA
                     GDNFVSSTDIYGGTWNLFQNTMSTMGIECRFVDPADPENFRKASDARTRCWYGETLPN
                     PKLAVFPIAEVAKMGREMGIPLIMDNTACPVLCRPLDHGAAIVMHSTTKFIGGHGTSI
                     GGVIIDGGNFDWEKHADRFPMLNTPDPSYHGAVWTQAVKPLGPIAYIIKARVTLLRDV
                     GAAASPFNCWMHIQGLESVVVRMRQHCANAQAVADYLAKHPKVSKVIYPSHMSGEPKR
                     RADAYLKGGFGGLCGFELKGGNDAGRKFIDSLNMFYHVANIGDARSLAIHPASTTHSQ
                     LTPEEQAKTGVTPGYVRLSIGIEHIDDIIADLDQALATA"
     gene            3872..5188
                     /locus_tag="VNR65_09115"
     CDS             3872..5188
                     /locus_tag="VNR65_09115"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HWJ18871.1"
                     /translation="MDTSSAPLPRESLSPAVLTALIALTILAGLYLAWPIWRATLPLQ
                     IDVNEAWNAYHADAIRDSLPLYPNPDGLVINNYPPLSFYVLAGLSALGVDPVYGGRLI
                     SLIALAALTGAIASLIRQFRASEMAAFLGAIWFCATMARFINSYVGMNDPSLPTVALM
                     TWGLVWLLRRQARGHAADPAIALIAVAGFYKHNYLAIPLTAILWVATSDRWAAVRAAL
                     VGAGAVALGLALCIELYGWVFVQSLLMAREYSLISGLGRLGRLQWIAPALVIIFVWAW
                     NRRGSETVRFSIIFVAIAFGTYFLQCLGAGVDDNAQFEVIIAAAVGLGFAFGGVAAIP
                     AMPWFTLERQRLTILIVVIVRLLLSLRTAPYLLVESPAFRAELHERAAVTQREIARVA
                     AIPGPVICDLDLVCRWAGKPKLIDSFFLAQSVATGRRTQAQVDRLQ"
     gene            complement(5378..5767)
                     /locus_tag="VNR65_09120"
     CDS             complement(5378..5767)
                     /locus_tag="VNR65_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18872.1"
                     /translation="MLDDRAASDGSTWDPALDAVIAAPANHKVVFENDHLRVLEVTLE
                     PGEEEPLHHHRWPSVFVFDQVQGPIHDIAPDGTQLPPNRDVMNALAKWDGKGCLVAEM
                     APQPLGRVLNASNKTVHGIRVEMKTSK"
BASE COUNT         1189 a         1680 c         1784 g         1386 t
ORIGIN      
        1 ccccggcatc tgcttcttca cattccactt gttgaccgcg gggtcgtatt cccagaccgt
       61 gccgtagggt gtgtggtcca cgtcgtcgag accggcgagt acgtaaatct ttccgttggc
      121 ggtcgcagca acgatttcgc ccatgggacg aggcatcggc gcggccattt tccaggcgcc
      181 cggcgtctgc gcctgcgcgc catacgcgac agccgcaagc ggcaccgcaa ccagcatcgg
      241 cacgagacgg cgacgtatga atgcacgcgt cgcattagtc agcccattgc tcattatcat
      301 tgaacgtttc cctgagatga ttgcctctat gacaggcctt acggcggtaa gattgcaccc
      361 ttccagcatt caccgcaaga ttttcggttg gtacacggac gtggatggcg ggtgtaggct
      421 aaagaggcga aaggcgaagc cagcgcagct ctatatgcta gcgagattga cctgaccgtt
      481 gttggagaat ggcttgtgtg acatcgacac tcaaccgata tcaacgcatc gccccagttt
      541 acgacctgct cgattttcca ttcgagtatg gtcgctaccg taagattagg ccgttgctgt
      601 ttgcaggact gtccggttta attttagatg caggcgttgg taccgggcgg aatttccctt
      661 tttatccgcg cgacagcaaa gttgtcggta tcgacctcag cccggctatg ctggcccgtg
      721 ccacgcgcag gcgtccgtca gcggttgctg aggtggagtt gcgtcaaatg gatgtcactc
      781 accttgactt tccagatcga ttttttgacg cggcggtcgc cacattcctg ttttgtgtgt
      841 tgcctgacga attgcaagtc gcggcgctca aggagattgg gcgcgttgtt aaaccaggcg
      901 gcagcattcg ccttttggaa tatacacgac cgcgcggatg gcttcggcgg gcagtgacaa
      961 gaatatggga gccatggatc ggttgggctt atggtgcaag tttcgaccgc cgcacggaag
     1021 agcgcatccc agaagctggc ttgcgccttt gcgaaacgcg ttttgtggta gacgacctca
     1081 ttaaaatgat ttccggctgt accgtcagga attgtcggga ttctagcccg gacgccacaa
     1141 gcattccccc ggggcagcac ctaaattgag cgcatgaaaa cgccgcacaa atccttggcc
     1201 gagccgtctt ctttcttttt cccgttccgt tcgcgattgg gatcgggaac cagcatctcg
     1261 gaagttggcc tgggtatctg gcctacgccg tagctgccgt ccttacaagg ggtggcaatt
     1321 atagtcggat gggcggcatt ccgggttcgc taattgttag acaaatccat gctatcctat
     1381 tgatcttcgt tatgcgatta gacactttaa aaatcgcaac tcactgtttt cacaattaaa
     1441 aaatcagatt gaaaatcgca gtgtcggtgg ttcgattccg cccctggcac cacaaattat
     1501 ccaataaata caagatttta ttggggttcg ccataactag atataaccag ataatttcct
     1561 atgggggcac gctgggggca caatcaattt gtcgaaaaag gccattgaag caggattggt
     1621 cgtcgtctca ggcgaactcg gatcttctat cggaagacgg caaataccca gtcattaaaa
     1681 gacgccaatt gaatgcagcg aataaagtgg tctgttgatt acgggcattc aagcgcatct
     1741 agcctgaaat tcaatcttgt aaattgacgg caagtaaccc aagcggcttt cgctaattgc
     1801 caatatgagc tggactgcca cgatcacgca actcgtggca tttccgtaat ttcatcacaa
     1861 gtcggtttca gcttcgtcgg gagcaaagtg tttaactgtc acggcatctt gtgaaatcgg
     1921 tcggagagcc ggatgcctta atcgggcacg tccggttcga tgagcgggga tgggaaacgg
     1981 agcgttgccg aatggcctaa gctaccgcgc caatcctcga ctctacctta gcgtcattcg
     2041 tggcgctgca ccatttctgg tcgctatcgg gccggacaaa acggccgtcc gaggtttgaa
     2101 ttgctatggc gctaatgacc ctcgacggaa atgcccagta tcgttcctcc gccagttgct
     2161 tcgcctacca aatatcagcg gttaatggct tcactatgcg gtcgcaagcg cctgatcgag
     2221 atcggcgatg atatcgtcga tgtgttcgat accgatggag aggcgcacat aacccggcgt
     2281 gacgccggtc ttcgcctgct cctccggcgt gagctgcgag tgcgtggtgc tggccgggtg
     2341 tatggcaagc gagcgggcgt cgccgatatt ggcgacgtga tagaacatat tcaggctgtc
     2401 gatgaacttg cggccggcgt cgttgccgcc tttcaattcg aagccgcaca ggccgccaaa
     2461 gccgcccttg aggtaggcgt cggcgcgccg cttgggctcg ccgctcatgt ggcttggata
     2521 gatgaccttg ctcaccttcg gatgcttggc gaggtagtcg gcaacggcct gcgcattggc
     2581 gcaatgctgg cgcatgcgca ccaccaccga ttccagcccc tggatgtgca tccagcaatt
     2641 aaacggcgag gcggccgcgc ccacgtcgcg cagcaaggtc acccgcgcct tgatgatata
     2701 ggcaatgggg ccgagtggct tgacggcttg cgtccacacc gcgccgtgat agctggggtc
     2761 cggcgtgttg agcatgggga agcggtcggc gtgcttctcc cagtcgaaat tgccgccgtc
     2821 gatgattacg ccgccgatcg aggtgccgtg gccgccgatg aatttggtgg tcgagtgcat
     2881 gacgatggcg gcgccgtggt cgagcgggcg gcagagcacc ggacaggcgg tgttgtccat
     2941 gatgagcggg atacccatct cgcggcccat cttggcgacc tcggcgatgg ggaagacggc
     3001 gagcttcgga ttgggcagcg tctcgccgta ccagcagcgg gtgcgcgcgt cgctcgcctt
     3061 gcggaaattc tccgggtcgg ccggatcgac gaaacggcat tcgatgccca tggtgctcat
     3121 ggtgttctgg aacagattcc aggtgccgcc gtagatgtcg gtcgaggaga cgaaattatc
     3181 gccagcgtgg cagatgttct gcaccgcgaa cagcgaggcc gcctggccgg aggacaccgc
     3241 aagcgcagcg acgccgcctt cgaacgcggc catgcgctct tccagcacgg cctgcgtcgg
     3301 gttcatgatg cgggtataga tgttgcccag ttccttgagc gcgaacaggt tggcagcgtg
     3361 ctcggtcgaa ttgaactggt aggaggtggt ctgatatatc ggcaccgcca ccgcggttgt
     3421 cgcgggatcg gcgcgatagt tgccgccgtg caaggcgata gtttccggat gcttgctgag
     3481 agtcggcata tttcccccca agctgacgct aaggccgctg gttttgacta tgagcccatt
     3541 tttgattacg gcccatagcg tattcaagct gcgctgggtg gtcaactctg tctgctattg
     3601 atggcgcttc acgtcattcg ccgcgacgca ccatttttgg tcgctcttgg agtaacaaca
     3661 gacaaagaac tgcccggagg ctgaaaagct acgcagcgaa tgacccactg cggacaacgg
     3721 cggatgtgca gccgcttgcg tgaatgtaat gttgtttgca gcaagctcat cgttttacta
     3781 aagctctgcc gttggggcgt ggatattcgt ttgcgacgac acgcatcgtt acgcgatgca
     3841 taagcgcgac agaaccgtgc ggagcgaata aatggatacg tcttccgctc cgctcccgcg
     3901 agagtcctta tcgcccgcgg ttttgacggc gctaatcgcg cttacaatcc ttgcggggct
     3961 gtacttagcg tggccgatct ggcgcgccac tttgccgctt caaatcgacg tcaacgaggc
     4021 atggaacgct taccatgccg acgcgatacg tgacagcctg ccgctttacc ccaatccaga
     4081 cgggctggtg atcaacaatt atccgccgct atcattctat gtgctcgccg gactttccgc
     4141 cctgggcgtg gatccagtct atggggggag gctgatttcg ctgattgcac tagctgcgct
     4201 tacgggcgcc atcgccagct tgatccgaca atttcgagca tcggaaatgg cggcgttcct
     4261 cggtgcaatc tggttctgcg cgaccatggc gcgcttcatt aatagctatg tcggaatgaa
     4321 cgatcctagt cttccgacgg tggcgctaat gacatgggga ttggtgtggc tgctgcgccg
     4381 ccaagcacga gggcacgcgg ctgatccggc tatcgccctc atcgcagttg ccggattcta
     4441 taagcacaat tacctggcaa taccgctgac cgcgattttg tgggtcgcga ccagcgatcg
     4501 ttgggcggcg gtgcgcgcgg cgctcgttgg agcgggcgca gtggcgctcg gcctcgccct
     4561 ctgcattgag ctttacggct gggtgttcgt ccaatcgctg ttaatggcgc gtgaatattc
     4621 attgatcagc ggccttggga ggctcggccg tttgcaatgg attgcaccgg cgctggtcat
     4681 tattttcgtt tgggcatgga ataggagagg cagcgagacc gtccgcttca gcattatatt
     4741 cgtagcgatc gcgtttggga cgtacttcct gcaatgcctg ggcgccggcg tcgatgacaa
     4801 tgcacagttc gaggtaatca ttgcggccgc agttggactc ggtttcgcct tcggcggggt
     4861 ggcagcgatt ccggcgatgc cttggttcac cctcgaacgg cagcgcctga ccattctcat
     4921 cgtcgtgatt gtgcggctgc tgctgtcgct gcggacggca ccctatttgc tggttgagag
     4981 ccccgccttc cgcgcggagt tgcacgaacg cgctgccgtg acccaaagag agatcgcacg
     5041 cgtcgctgca attcccggcc cggtaatctg tgacctagac ttggtttgtc gatgggctgg
     5101 aaagccgaaa ctaattgact cgtttttttt ggcgcaaagc gtcgccaccg gacggcggac
     5161 gcaagcgcag gtcgaccggt tacagtaggc gctcggcgcg ctccacgtgc gggtggaccc
     5221 gcgcgccttc gccgagtggc cttgaaggcc tggttcggca agaccactct tgccgaaacc
     5281 gctactcggg caccgtcatc ggctcagaag tattggtccg ggcgacgaac ttcagccgcg
     5341 aggtggaaat catacttttt tcttcctctt aacggactta tttcgatgtc ttcatctcaa
     5401 cacggatgcc gtggacagtt ttattcgacg cgttcaggac gcgaccaagc ggctgcggtg
     5461 ccatctctgc gactaggcac cccttcccgt cccattttgc gagcgcattc atcacgtctc
     5521 tattaggcgg tagctgagtt ccatcaggtg caatgtcgtg gatcggaccc tgtacctgat
     5581 cgaacacgaa aacagatggc caccggtggt gatgcaaggg ttcttcctca ccgggctcca
     5641 aagttacctc aaggacccgc aaatggtcgt tttcgaaaac gaccttatgg ttggcaggcg
     5701 cggcaatcac ggcatccaat gcaggatccc aagtagagcc gtctgaagcg gcacgatcat
     5761 ccagcatgac aacctccata ggttagcccg acgcgaccga ggccaatgat tgcttttcct
     5821 ccgcgggctc aggtcaacct gtaggctatc attaagcggc ccgaccgcaa gcgcgcgttg
     5881 gctaggtcgc gatggcagct tttggcactt agcgtcgttc gccgcgatgc aaccaacatg
     5941 gtcgctatcg gaccaacagc ggacatttca gcccatctac ctgcatcatg ctggtgggct
     6001 gaacgagtcc tagctgacgc tcagtccagc atcatgtcg
//
LOCUS       DATMGX010000558         2042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_161441, whole genome shotgun sequence.
ACCESSION   DATMGX010000558 DATMGX010000000
VERSION     DATMGX010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161441"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VNR65_09125"
     CDS             complement(<1..412)
                     /locus_tag="VNR65_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWJ18873.1"
                     /translation="MRFYNPIAILVAALIFGISPALAQQPQNPQANPLDNVPDKMPFN
                     IPYGAPITAARAQTVIQAAEQEANKRGWPLNIAVVDSGGNLVSFLRMDGAQLASIAIS
                     EHKARASVKYRRATRVYEDAVQKSNYKYILSLDDA"
     gene            complement(1153..1785)
                     /locus_tag="VNR65_09130"
     CDS             complement(1153..1785)
                     /locus_tag="VNR65_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HWJ18874.1"
                     /translation="MKSQEEQMNLDLVENAFVDPSFEITRQIARDCRACHLWEPATQT
                     VFGEGPVPAPVLLIGEQPGDKEDLAGRPFVGPAGLKLNEALEEAGIERQKVYVTNAVK
                     HFKYVPRGKIRLHQKPVTVEIKACRPWLERELDFVQPKVAVAMGATAAQSLFGKAMPI
                     NKNRGRLVDLGSCQALITIHLLRIQEEADAGAEYARFVADLKLLLPFLVE"
BASE COUNT          423 a          575 c          559 g          485 t
ORIGIN      
        1 tcgcgtcgtc gagactcaaa atatacttgt agttcgactt ctgcacggca tcctcataca
       61 cccgagttgc gcgtcgatat ttgaccgaag cccgcgcttt atgctcggag attgcgatgg
      121 aagccagttg agctccgtcc atccgcagga atgagacgag gttgccgccg gaatcgacca
      181 ccgcaatgtt tagaggccat ccgcgtttgt ttgcctcctg ctccgccgcc tggatcacgg
      241 tttgggcgcg ggccgctgtg atcggcgcgc cgtaaggaat attgaacggc atcttgtccg
      301 gaacattatc aagaggattg gcttgcgggt tttgtggctg ctgtgcaagc gcagggctga
      361 tcccgaagat caatgctgcg accaatatcg caattgggtt gtaaaatcgc atcgttgctg
      421 accttaagtt gccgcgcggc ctcgtagtta gaactgccgc cgcaaatata ggttcctaac
      481 tctggcattt tgtctggatc ccgcgaatgg tggcatctgt ttgtcaactt gcccctgacg
      541 ctgtcgttcg acttccttct tctgcagatt gtcagcgaaa tcgccttgcc gtcaggttaa
      601 tgagagcttc agccctctcc aatgcctgac aatgcgagga aaaataccga acgagacgtc
      661 atcgcggccg cctacaccac cgctgttccg gctgagcgca agcgttaatt tgcgaagccg
      721 aaacatattt aacggacttt agagcgtccc aataaaggga aaaattccag cattaaattc
      781 tatttaatgg gaggtcacat gtcaggaaat gctgttctct atcaatagcc tagcgcaatt
      841 acttcccgtc acatcgagcg cgcggcgctc ttcttttccg tccgaatgaa cggatcttta
      901 agcatatgat gaccggaaat aaatccggcg gatttctgtt aaccatgtcc gggttaacgt
      961 gtaggcgcgg caacgggttg tttacccttt tcgccacgca aagctccgcc cccgtcgcga
     1021 tgagaaaacg aggcgctgag tgagatgcga tcgcttggcg cactcccaca aattttgttc
     1081 gtcggccgca tcagtttgat ggagcttggg agtcgagcgc gaactcgtgg cagctgatcc
     1141 caaatgtcaa attcactcca cgagaaatgg caggagcagc ttcaggtcgg cgacgaaccg
     1201 ggcgtattcc gctcccgcgt cggcctcttc ctgaatgcgc aaaagatgga tcgtgatcag
     1261 tgcctgacag ctgccgagat cgacaagccg tccacggttc ttattgatcg gcatcgcctt
     1321 cccgaacagc gattgcgccg ccgtcgcgcc catcgcgacc gcgactttcg gctgaacgaa
     1381 atcgagttcg cgctcgagcc atggtcggca cgctttgatc tcgaccgtaa ccggcttctg
     1441 gtgaaggcgg attttgccgc gtggcacata tttgaaatgc ttcaccgcgt tggtgacata
     1501 gaccttctgc cgctcgatcc cggcttcctc aagcgcctcg tttaatttga gtccggcagg
     1561 gccaacgaac ggccgtccgg caagatcttc cttgtcgccg ggctgctcgc cgatcaggag
     1621 cacgggcgcg ggcacaggcc cctcgccaaa tacggtctgc gtcgcgggtt cccagaggtg
     1681 gcaggcgcgg caatcgcggg cgatctggcg cgtgatctcg aacgatggat cgacgaaggc
     1741 attctcgaca aggtcgagat tcatctgctc ttcttgtgat ttcatcgcgg cgcctttgat
     1801 cgatcgttca gtcctggcca agcggcggcc cttgtttccc ataacaccgg agcgcaaaca
     1861 tcgtttatcc cgctctccgc ggacgcataa gcttgtaaat tacgggggcc caaacgttga
     1921 cttcatcctg atctgctttg aaaaggcata cggcaatgcg tttcacttta atcgagattg
     1981 gggccaacca aaatgttaga gcgccgtgca gcgtggggtt ggcgtttcgt cgagcgctcc
     2041 ct
//
LOCUS       DATMGX010000559         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_298073, whole genome shotgun sequence.
ACCESSION   DATMGX010000559 DATMGX010000000
VERSION     DATMGX010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298073"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..528)
                     /locus_tag="VNR65_09135"
     CDS             complement(106..528)
                     /locus_tag="VNR65_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18875.1"
                     /translation="MPDALRSIPQISQAPAESKPCTSLAGKRILVVEDDGLIALDIIS
                     ELRRAGCTAIGPASRLETAMTMAAAEHIDAAVLDVFLGGAYAWQLAGTLKAQGVPFLF
                     QTGFGRFLDFPVEFASVPRLEKPVMTGVLKRELKALLA"
     gene            complement(573..1067)
                     /locus_tag="VNR65_09140"
     CDS             complement(573..1067)
                     /locus_tag="VNR65_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18876.1"
                     /translation="MYFIIEYVDTATGIEGEPLSWEFAKTEGEATIKARAHLLLFKTR
                     CGAQGYRILSPAGALVAYGPGAQEEDADYPVAIEQLPKGHLGETHDRFSTNGLHDSED
                     LDWRSPGQGSPFVYSKTILARVAKGTAFRRRKSAEHQRRHACQSRTQAAAFTSARKSP
                     PGRL"
     gene            1617..1988
                     /locus_tag="VNR65_09145"
     CDS             1617..1988
                     /locus_tag="VNR65_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ18877.1"
                     /translation="MMNPAKILVVEDEFLVAIQIESILTGAGWRVIGSAGTLASAVSL
                     ARRSACDAAVLDVNLRGERVDEVASILSERSIPFLFVSGYGRGNLPAAFRDGVEFLAK
                     PFSDQMLVQTVGDLLRNRRLS"
BASE COUNT          465 a          590 c          596 g          477 t
ORIGIN      
        1 atcacacgct attatgcgta agctcgtcgt tctcgcttac gccctgctca gcaaaaaccg
       61 catgtggacc caaaaacccg attgatcaac accgatactc tagtcttagg ctagaagcgc
      121 tttgagctcc cgcttcaaga cgccggtcat cacaggcttc tcaaggcgag gaacagaagc
      181 gaattcgact gggaagtcga ggaaccgacc aaagccggtc tggaaaagga aaggaacccc
      241 ttgcgctttg agcgttcccg ccaactgcca tgcatatgcg cccccgagga aaacgtcgag
      301 cacagccgcg tcaatatgct ctgcggctgc catggtcata gctgtttcca gtctcgaggc
      361 gggtccgatc gcggtgcatc ccgcgcgtct gagttcactg atgatgtcga gagcgataag
      421 cccgtcgtcc tcgacaacaa ggatgcgctt accggccaag ctggtgcatg gcttagactc
      481 cgccggagct tgtgatatct gcgggatgga tcgcagagca tccggcatgg tctcctctag
      541 cagtatgggc ttcgaactcc gtggttcaga tatcacagcc gccccggagg agacttgcga
      601 gcgcttgtga atgctgccgc ctgcgtccgc gattggcaag catgccttct ctgatgctct
      661 gccgactttc tcctgcgaaa agctgtgcct ttcgcgaccc gtgccaagat ggttttggaa
      721 taaacgaacg ggctaccctg acccggactg cgccaatcga gatcctcgct atcgtgcagc
      781 ccgttcgtgg agaagcgatc atgcgtctcg ccaagatgcc cctttggaag ctgctcgatc
      841 gccacgggat agtccgcgtc ctcttcctgc gcgcctggtc cataggcgac aagcgctccc
      901 gctgggctta gaatgcgata cccctgcgca ccacatcgag ttttgaaaag aagaagatgg
      961 gccctggctt tgatggtggc ctcgccttcc gtcttagcga attcccacga caagggttcc
     1021 ccctcgatcc cggtggcggt atcgacatat tctatgatga agtacatgcc cactgattcc
     1081 gttgatgggg cattatatag cgaataacaa gttttgatcc aggccgcgac aacctgaagc
     1141 acagcgttgg attttgccgg tgggcattga acaaacgatc gcccgaaatg gaagataact
     1201 acctctcttt aaaatgcata aaacagcaaa acttggtctg gccgtccggt gacttcgcag
     1261 cagaaggtgg ccgcatcaaa caacctaata aaggtatctt gttgctacga aatcttacga
     1321 aagtcggagg aacggcgcca agtttcatgc agctgaattg ccgttcgtcc ggtgaaactt
     1381 cgcgcgctcc ttaaaggaga ggtccctgct gaagtattcc tcgggcgtca cagcctcgat
     1441 ccggtggggc attttaggtg acggccgctc atccctgccg tcacggcttt gtgttaaccc
     1501 attccttttc gacagtggtt tgttggacag tcaacccgct attatcagtg aaggttttcg
     1561 gtataccagc attaagagga acaggcgcta aggcgtaaag tccctcggtg agcgcaatga
     1621 tgaacccagc aaaaatcctt gtggttgagg acgaatttct cgtcgccatt cagatcgagt
     1681 ctatcttgac cggtgcggga tggagggtca ttggatcggc cggaactctt gccagcgccg
     1741 tcagcctggc tcgaagaagt gcgtgcgatg ctgctgtgct ggatgtcaac ctgaggggcg
     1801 agcgcgtcga cgaggtggcg tctattcttt cggagcggag tatccctttc ctgttcgtat
     1861 ccggctatgg gcgcggaaat cttccggcgg cgttccggga cggcgtcgag ttccttgcaa
     1921 agccgttcag cgatcaaatg ctcgttcaaa ccgttggaga ccttctgcgg aatagaaggc
     1981 tctcgtgacg ccagtcgggc aacttgtctg aagcagcggc ggccaaatcg cagcaaagca
     2041 gaaaaaagcg gttggtgcga gaccaaccgc ggcgagtgct agagcatcgc ccgcttaatc
     2101 cgacgcgcgc atccggggct ttttgtat
//
LOCUS       DATMGX010000560         2353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_335559, whole genome shotgun sequence.
ACCESSION   DATMGX010000560 DATMGX010000000
VERSION     DATMGX010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335559"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VNR65_09150"
     CDS             <1..1352
                     /locus_tag="VNR65_09150"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWJ18878.1"
                     /translation="RELSETLNNSDSESIRQSIWPVGTRVHALSGARTLLPLFEIRTS
                     RQRFAIRREHEEQQLGEISLDETVISRPQGAPLTSMQCVEVEALTEAHEPLQTLVRTL
                     QRDCALEAAFDTKFSYGLRSAGLVPVPAPQFAPAAIDASMSMLEVVVANLRRHLSAWH
                     RHEPGARLGDDPEELHDLRVAGRRLDSILRQFRSSLPASFPRIRPALKTVMRALGHVR
                     DLDVALSELDFFSREAPKSDRNSVEPLKQHLVSERDRARARMLSVLDSIWMRKNLQEL
                     WSLLAAPSAPSHHSSPESALNVSPKLIRRRYRKLRKSADLLRSDSSMEAYHGVRGQVK
                     KLRYALEAVAVIYGKPADEMLRALRRWQEKLGVQQDAAVAGLRLKAIAGAPPKGIPPE
                     TLFLMGRLAEHYASIAVQARKRHAKTYRRVRERWKRLRMKFEDLAPNDAPKLPDSGR"
     gene            1383..2150
                     /locus_tag="VNR65_09155"
     CDS             1383..2150
                     /locus_tag="VNR65_09155"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWJ18879.1"
                     /translation="MKIIAVTNIKGGVGKTTTAVNLSYLCAASGRPTLLWDLDPQGAA
                     TYTLRGEPLERASAKKLLGGKRHLPELVLSTGYPSLDLLPADFSYRNFDVHLSERKRP
                     TERLLKMSRSLREVYSALFLDCAPGISLLSENVLRAADAIIVPLLPTPLSVRMLAQLR
                     DFIAGEGWVDLMLLPFFSMVDRRKSLHHDVIANTREQFPTMLATEVPYWSEIERMSLR
                     RAPIPAFAPSGPAAQIYAALWAEVAARMGDLPPTTRQ"
BASE COUNT          474 a          663 c          734 g          482 t
ORIGIN      
        1 gccgggaatt gagcgaaacg ctgaacaatt ccgacagtga gtcgattcga caatcgattt
       61 ggccggttgg aacgcgcgtc cacgccctta gcggcgcgcg cactttgctg cccttgtttg
      121 aaatacgcac ctcaaggcag agatttgcga ttcgcagaga acacgaggaa cagcagcttg
      181 gcgagatttc ccttgatgaa accgtcatct cgcgcccgca gggcgcgccc ctgacgagca
      241 tgcagtgcgt cgaggttgaa gccctcacgg aggcccacga gccgctgcaa acgctggtga
      301 ggacgctcca aagggactgc gcacttgagg cggcgttcga cacaaagttc tcgtacggcc
      361 tcaggtcagc aggcttggta ccggtgcccg cacctcaatt cgcgccggcg gcgatagacg
      421 cctcgatgag tatgctcgag gttgttgtgg cgaacctgcg tcgccacttg tctgcctggc
      481 atcggcacga acccggtgcc cgcctgggag acgatccgga ggaattgcat gacttgcgcg
      541 tcgccgggcg cagattggat tccatcctgc gacagttccg atcgtctctg cccgcatcgt
      601 ttccgcggat tcggccagcg ctcaagacgg taatgcgtgc tctcgggcac gtacgagatc
      661 tggacgtcgc cctgagtgag ttggatttct ttagccgcga agcgccaaaa tcggatcgca
      721 atagcgtcga accccttaaa cagcacctgg tgtccgagcg cgatcgggct cgcgcgcgta
      781 tgttgagcgt tctcgattca atctggatgc ggaagaatct gcaggaattg tggtcgctgt
      841 tggccgcacc ttctgcgcca tcccatcact catcgccgga gtcggcgctg aacgtgtcgc
      901 cgaaattgat ccggcgacga taccgcaagt tacgcaagag cgcggacctc ctgaggtcgg
      961 attcctcgat ggaggcgtat cacggggtcc gcggccaagt gaagaagttg cgctacgctc
     1021 tggaagcagt ggcggtcatc tacggaaaac ctgctgatga gatgcttcgt gcgctgcgcc
     1081 gctggcagga gaagcttgga gtgcaacagg atgccgccgt ggcgggcctt cgcctgaagg
     1141 ctatcgccgg cgcgccaccc aagggtattc cgccggagac gctgtttttg atggggcggc
     1201 ttgccgagca ttatgcgagc atcgccgtgc aggcgcggaa gcgacatgca aagacttatc
     1261 gaagagttcg agaaagatgg aaaagacttc ggatgaagtt cgaggatttg gctccgaacg
     1321 acgcaccgaa gcttcccgac tccgggcggt gaggcgatgg aaatatcaat taagccggct
     1381 cgatgaagat aatcgccgtc acaaacatca agggcggggt cggtaagacg actacggccg
     1441 ttaacctctc gtatctgtgc gccgccagcg gtcgacccac cctgttgtgg gatctggacc
     1501 cgcaaggtgc cgcgacttac acactgagag gcgaaccact cgaacgcgct tcggcaaaga
     1561 aactgctggg tggcaagcgt caccttcccg aactggtcct gtcgacggga tacccgagtc
     1621 tggatctttt gccggcggat ttctcctatc gtaattttga tgttcacttg agcgagcgca
     1681 agcgacccac cgaacgcctt ctgaaaatga gccgatcatt gcgagaagtt tattcagcgc
     1741 tatttctcga ttgcgcgccc ggcatctcac tgttgtcgga gaacgtgctg cgcgctgcgg
     1801 acgcgatcat cgtaccgctg ctccccacgc cgttgtcggt gcgcatgctg gctcaattgc
     1861 gggactttat tgccggcgag ggctgggtgg atttgatgct tttgccgttc ttctcgatgg
     1921 tcgatcggcg caaatctctg caccacgacg tcattgcaaa tacgcgcgag cagtttccaa
     1981 caatgctcgc gaccgaagtg ccgtactgga gcgagattga acgcatgagt ctccgccgtg
     2041 cgccgatacc tgctttcgcg ccgagcggcc cggcggcgca gatctatgcg gcgctttggg
     2101 cggaggttgc ggcgcgaatg ggagatttgc cgccaacgac ccggcaatag gaattcgccg
     2161 tcgcctttta gccatggtgc ccgcactttc cggcagggat caggtagctg ccgtcttgcg
     2221 agggcgagcc gtcctggctc gcgtatgagg tgaagctggg gcggggagcg gctacgggcg
     2281 cttgaaatga gcgctcggtg gcgggaaggg accagataat gtattaataa caacaagata
     2341 tattagataa cta
//
LOCUS       DATMGX010000561         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_116196, whole genome shotgun sequence.
ACCESSION   DATMGX010000561 DATMGX010000000
VERSION     DATMGX010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116196"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VNR65_09160"
     CDS             complement(<1..580)
                     /locus_tag="VNR65_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HWJ18880.1"
                     /translation="MDTILVANAGSSSVKFEVFGVDQSGGLSSQLEGKIEGVGTKPHL
                     TAVSAEGKVLVERTYPTETVPDVPTALHTGGAWLRDELLIHPIAVGHRVVHGGPEFDQ
                     PVLVTEDVLHRLERYIPLAPLHQPHNLEPIRSIMVNRPGLPQVACFDTAFHRKHPAVA
                     DYYAIPEHFHAEGIRRYGFHGLSYEYIAARLVE"
     gene            complement(586..1548)
                     /locus_tag="VNR65_09165"
     CDS             complement(586..1548)
                     /locus_tag="VNR65_09165"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HWJ18881.1"
                     /translation="MSSEAVEERAPHEKYARLMARAAVVKSPRTIVVYPCDEASLTGA
                     VEAAEAELIEPLLVGPAEKIKAVANKFGLDISQFEIVQAEDGEEAAEKGVQLIQKAKG
                     EVLMKGSLHTDELMRSVTSKTSGLRTARRISHAFIMDVPTYKETLLITDAAINIFPDL
                     EAKRDIVQNAIDLYTHISQGVPRVAILSAVEIVTPKIPSTIDAAALCKMADRGQITGG
                     ILDGPLAFDNAVDPEAAAIKGIKSEVAGRAQVLVVPDLEAGNMLAKNLIFLLKADSAG
                     VVLGARVPIILTSRADSLRARLASCAVAALSADARRREVGLPAA"
     gene            complement(1545..>2115)
                     /locus_tag="VNR65_09170"
     CDS             complement(1545..>2115)
                     /locus_tag="VNR65_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18882.1"
                     /translation="AAAGVDPKSSEPQRMAPQSTLHRELIQDRIAELKSRIRRGGVQE
                     CTIRGLIYIGLARGGVDERGVNALKQIRLSDATPRLTLDEFKDMARDQFFMLLLDTEA
                     TLEAIPVLLPADAALRQNAFDSIKKVLEASGELTGEAVERLNRVKSLFRLGATGAWLR
                     GESLKLVPKLEPSENTRTPVANPGTGRKS"
BASE COUNT          398 a          659 c          624 g          434 t
ORIGIN      
        1 cctccaccaa gcgcgccgcg atgtattcgt aagacaggcc gtgaaatccg tagcggcgga
       61 tgccctcggc atgaaaatgc tcaggaatag cgtagtaatc ggcgacggca ggatgcttcc
      121 gatgaaacgc cgtgtcgaag catgcgacct gcggcaaccc cggccggttg accatgatcg
      181 atcggatggg ctccagattg tgcggctgat gcagcggcgc gagcgggatg tacctctcga
      241 ggcgatgcag cacgtcctcc gtcacaagaa cgggctgatc gaattccggg ccgccatgca
      301 cgacgcgatg gccgacggcg attgggtgga tgagaagctc gtcgcgcagc cacgcacccc
      361 ctgtgtgcaa cgcggttgga acgtcgggca cggtttcagt cggatatgtc cgctcgacga
      421 gaaccttgcc ctcggcggac acagcggtca gatgcggttt tgtcccaacg ccttcgattt
      481 tgccttcgag ttgactgcta aggccgcccg actgatcaac accgaacacc tcgaacttga
      541 cgctcgaaga cccggcattt gcaacgagga tcgtgtccat cgcgcttaag ccgccggcaa
      601 gccgacttca cggcggcggg cgtcggcaga aagcgccgcg accgcgcacg aagccaagcg
      661 ggctctcagc gagtccgcgc gcgatgtcag aatgattgga actctcgcac ccagaacgac
      721 accagcggaa tccgccttca agaggaagat cagattcttg gcaagcatat tgccggcttc
      781 caaatccggg acgacgagca cttgcgcccg gccagccacc tccgacttga tgcccttgat
      841 cgccgcagct tccgggtcga cggcgttgtc gaaagcgagc ggcccatcga gaatgccgcc
      901 ggtgatctga ccgcgatccg ccatcttgca gagcgctgcc gcgtcgatgg tggatgggat
      961 tttcggcgtc acgatttcga cggccgacag gatcgcaacc cggggcaccc cctgcgatat
     1021 gtgcgtgtaa agatcgatgg cgttctggac gatgtcgcgc ttggcttcga gatcggggaa
     1081 tatgttgatc gccgcatccg ttatgagcag tgtctctttg taagttggaa catccatgat
     1141 gaatgcgtgg ctgatgcggc gtgccgttcg aaggccggag gtcttcgatg tgacgctccg
     1201 catcagctca tcggtgtgca ggctgccctt cataaggact tcgcctttcg ccttctggat
     1261 gagctgaacg cccttctccg cggcttcctc gccatcttcc gcctggacaa tctcgaactg
     1321 gcttatgtcc aggccgaact tgttcgcaac cgctttgatt ttttccgccg gcccaacaag
     1381 caagggctca atgagttcgg cttccgctgc ctcgacagcg cccgtgagcg aggcctcatc
     1441 gcacggatag acaacgatcg ttcttggcga cttaacgacg gccgcgcggg ccatgagccg
     1501 cgcatatttt tcgtgcggcg ctctttcttc aacggcctcc gaactcatga tttcctccct
     1561 gttcctggat tcgcaacagg ggttcgagtg ttctcggaag gctccagctt cggcacgagc
     1621 ttcaaggact ccccccgcaa ccatgctcct gttgccccga gccgaaagag acttttgacc
     1681 ctgttcagac gctcgacggc ctcgcctgta agctccccgc tcgcctccag aaccttcttt
     1741 atggagtcga aagcgttctg acgcagcgca gcgtccgccg gcagaagaac cgggatcgcc
     1801 tccagtgttg cttccgtatc gagcagcagc atgaagaact gatcgcgcgc catgtccttg
     1861 aattcgtcaa gggtgagccg cggcgtcgca tccgagagcc tgatttgctt gagcgcattg
     1921 acgccgcgct catcgacccc gccccgagcc aggccaatat agatcagtcc gcgaatggtg
     1981 cattcctgta cgccacccct tctgatgcgc gatttgagtt cggcgatgcg gtcctgaatg
     2041 agttctctgt gcagggtgga ttgcggcgcc atgcgctgcg gctctgacga tttcggatcg
     2101 acgcccgccg cggcc
//
LOCUS       DATMGX010000562         2063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_324043, whole genome shotgun sequence.
ACCESSION   DATMGX010000562 DATMGX010000000
VERSION     DATMGX010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324043"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VNR65_09175"
     CDS             complement(<1..1200)
                     /locus_tag="VNR65_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWJ18883.1"
                     /translation="MTQREKFETKKLPMMPIRDVVIFPYMMTPFVVGRESSVRALEEA
                     LAGDKKIFLATQHDASIDEPKPNEIFQVGTLVNIVQSLKLPDGNIKVLVEGLERGKIL
                     QVVDTDGYFEATVRTAKYSAEPTPQIETSMTRVTGLFEQYVKLCQSLNYETMIAAVRN
                     DDPAKLTDTIAANLQLSIEEKQELLEIFDPAERLNRIGDVLDVEIEKLNMDRTIQSRV
                     KRQMERAQKEYYLNEKIKAIQKELGRGEKSEFDELKKKIDTAGMPRETHEKALQELKK
                     LEAMPPMSAESTVSRNYLDWLLAVPWKKRSKEIRNIDIAEKTLNEDHYGLEKIKERIL
                     EFLAVRQLVKNPKGSILCFVGPPGVGKTSLGMSIAKATGRKFVRLSLGGIRDEAEIRG
                     HRRTYIGA"
     gene            complement(1603..>2063)
                     /locus_tag="VNR65_09180"
     CDS             complement(1603..>2063)
                     /locus_tag="VNR65_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWJ18884.1"
                     /translation="RSGEDADKEDAITSKKRTQELLTEIQPEDLIKFGLIPEFVGRLP
                     VVGMLEDLDEFALIEILTRPKNAIVKQYQRLFEFENVKLRFTDEALRCISKEAMLRKV
                     GARGLRMILEELMLDLMYNLPGQKRVKDFEVTAEMVQKRNVNLPIMEKAG"
BASE COUNT          388 a          584 c          593 g          498 t
ORIGIN      
        1 cgcgccgata taggtgcggc gatggccgcg aatctcggcc tcgtcgcgaa ttccgcccag
       61 cgacaggcgg acaaacttgc ggccggtggc tttggcgatg ctcatcccca gcgaagtttt
      121 gcccacgccc ggaggcccga cgaaacaaag gatcgagccc ttcgggttct tgaccaattg
      181 ccgcaccgcg agaaactcaa gaatgcgctc cttgattttc tccagaccgt agtgatcttc
      241 gttgagcgtc ttctcggcga tatcgatgtt gcgaatttct ttggagcgct tcttccaagg
      301 cacagccagc agccaatcga ggtagttgcg cgataccgtg gattcggccg acatgggcgg
      361 catggcttcc agcttcttga gttcctgcag ggccttctcg tgggtttcgc gaggcatgcc
      421 ggcggtatca atcttctttt tcagctcgtc gaactcgctc ttctcgccgc ggcccagttc
      481 tttctggatg gccttgatct tctcgttgag gtaatactct ttctgggcgc gctccatctg
      541 gcgctttacg cgcgactgga tggtgcggtc catgttgagc ttctcgatct cgacgtcgag
      601 cacgtctccg atgcggttca ggcgctcggc gggatcgaag atttccagca gctcctgctt
      661 ctcttcgatc gagagttgca ggttggcggc gatggtgtca gtcagctttg ccggatcgtc
      721 gttgcggacg gcggcaatca tggtttcgta gttcagcgac tgacagagct taacgtactg
      781 ttcgaacaga cccgtcaccc gcgtcatgct ggtttcgatc tgtggcgtcg gttcagccga
      841 atacttcgcg gtgcgtaccg tggcctcaaa gtagccatcg gtgtcgacca cctggaggat
      901 cttgccgcgc tccaagccct ccaccagcac tttgatgttg ccgtcaggaa gcttcaggct
      961 ctggacgatg ttgaccagcg tgccgacctg gaaaatctcg ttgggcttgg gctcgtcgat
     1021 actcgcgtcg tgctgcgtgg caaggaaaat cttcttgtcg ccggccagcg cctcttccaa
     1081 tgcgcgcacg ctcgactccc ggccgaccac gaacggcgtc atcatatagg gaaagatgac
     1141 gacatcgcga atcggcatca tggggagttt cttggtttcg aatttctcgc gctgggtcac
     1201 tattaccacc tttgtcgttt cagagggccc atgccgctct gagaaagaag agctaatcgc
     1261 ttgcgctgca ttccgcgcct ggctatccgt tgcggccaag gtgagtcaag gataacacgc
     1321 gaaacaaggg ggccgaagcg aacaaaggcc cctgtcgtca atagcttccc ttgaatttac
     1381 atttgatccg atgccacatt ctgcgggaac gaggaattac gggaactgat atgtatatgg
     1441 atgcctctct ggctgggcgg attcatgcca atcgggagca aaacactggc gcgatcttag
     1501 ccgcgccgag tcctgaaaac gccctgattg cttgctgaaa ggggatagct cacgatgcca
     1561 gatgctacgt cgaacgtggc cgctgacaac tggccgcttc tcttagcctg ctttctccat
     1621 gatgggcagg ttcacattgc gcttctgaac catctcagcc gtgacttcaa agtctttaac
     1681 tcgcttctgg ccgggcaggt tgtacatcag gtcgagcatc agttcttcca gaatcatgcg
     1741 caggccgcgg gcgccgacct tgcgcaacat ggcttctttg gagatgcagc gcagggcctc
     1801 atcggtgaag cgcagcttga cgttctcgaa ctcgaacagg cgctggtatt gcttgacgat
     1861 ggcattcttc ggacgggtga ggatttcgat gagcgcgaac tcgtcgagat cttccagcat
     1921 gccgacgaca ggcaagcggc cgacaaattc gggaatcagg ccgaacttga tcaagtcttc
     1981 gggctggatc tcggtcaaca gttcctgggt acgcttcttg gaagtgatcg catcttcctt
     2041 gtcggcatct tcgccgctgc gga
//
LOCUS       DATMGX010000563         4510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_83921, whole genome shotgun sequence.
ACCESSION   DATMGX010000563 DATMGX010000000
VERSION     DATMGX010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83921"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            621..845
                     /locus_tag="VNR65_09185"
                     /pseudo
     CDS             621..845
                     /locus_tag="VNR65_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            1531..2889
                     /gene="istA"
                     /locus_tag="VNR65_09190"
     CDS             1531..2889
                     /gene="istA"
                     /locus_tag="VNR65_09190"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HWJ18885.1"
                     /translation="MNSQRVSIRETARLLRLSRQVIRQVLRSNSTLVPEIHRAEKAEP
                     YRQQVLDLLSTCKGNLVRVHEELTASGALLSYQALTAFCRRHGLGQTPSEPAGQYHFE
                     PGVEMQHDTSPHEVEVGGKAYKVQTASAVLCYSRLLFFQINPTFQRFDCKVFLTEALR
                     YMGGTPKQIMIDNTHVVVLRGTGRAMIPVPEMEAFAERFGFDFVAHERGDANRSGRVE
                     RPFSFIENNFLAGRSFSSWNDLNHQARQWCDRVNATYKKHIRAVPRELFAVERLHLKP
                     LPAWIPEVYRLHERTVDVEGYVSLHATRYSVPVSWIGRRVEIRETSDKVEIELDARHL
                     VTHVRTMAPLQRITLAEHKPPRGERVKLIERHPEEQAILHAAPEIAGYVAELKQKGRK
                     VVALSLRQLLRMLREYPRESFLAAVREAAQYGLYDLDRLERMILRRVARDYFLLDTDT
                     HD"
     gene            2882..3607
                     /gene="istB"
                     /locus_tag="VNR65_09195"
     CDS             2882..3607
                     /gene="istB"
                     /locus_tag="VNR65_09195"
                     /inference="COORDINATES: protein motif:HMM:NF038214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HWJ18886.1"
                     /translation="MTEELEQLLKNLKLRRMLHVYDEQLQAAEKSNPSYSEFIAGLLR
                     AQWHERQESALEWRIRKANLPERWSLETFPYARQPGVNRKQIRAFAELDFVAKHENLV
                     LVGPTGVGKTGLACGLLLKALQNGHRCQFIRAQDLFDEMYASLADRSTRQLLNRLARL
                     DVLLIDELGYLNLKPEQSNTFFKLMEERYHRHSTIITTNLIYDEWHSFLGNKSMVDAL
                     LSRVRHYCHTVTINGPSLRDPQG"
     gene            3618..3938
                     /locus_tag="VNR65_09200"
     CDS             3618..3938
                     /locus_tag="VNR65_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18887.1"
                     /translation="MEQREYVRRLLDAYRATPGTAGVVRRADRVFAAQLHARAVPFEV
                     VENALTLAAARRLARPTDAPPLGVIRSLAYFSPVIEEVLQTQPSPEYFRHLRQRMAKL
                     TSAR"
BASE COUNT          925 a         1373 c         1363 g          849 t
ORIGIN      
        1 agggctcggg atcatcctcc agaccactcg gaaacgaggg gaaggcccga tcgctagacc
       61 taaaccttcg gcgctcgcga gaagttaccc acctgtgctc tgcaaaactg acccacctgg
      121 catgaactaa gaactccgcg gcagccgctg gcgagtggtg acgatagtca tcaccatgcc
      181 agaggcccgg aagagaccct gttccatatg ccgccggtgg tttcggccgg acccgcgagt
      241 tggttcgcga cagcatgcct gcggtcaggc cgagtgccag acggctcgcc ggcaaaaaac
      301 acaagccggt tggcggcgcc ggaatcccgg ctatgccatc gcctaccgca tggatcggcg
      361 ggccgctcag aaagagcccg aaccggaacc gcttcgtcta cccgctccgc tgactcaact
      421 accgtgggat ctcgcgaaag accagttcgg cacgcaaggg gctgatttcc taggggttat
      481 gggaggactt ctggtgcggt ccgcgaaaga ccagttccgg gcttatgttg ttgattcgga
      541 gagattttcc ggcacacttc ccgtcccgcc gcgaaagacc agtccggcct tcggccatac
      601 tgaaccccga accgacgacg atgcaactgg agtttcatca gctcgatcag cgctgggaac
      661 atctgcgagt ccgccccgcg gcccagctgc ggcagttgat ggcctccttg gctgaagcgg
      721 gtcaacaaac gccgatcgtc gtggtggcct ccgaagagcg ggacggccgc tatctggtga
      781 tcgacggtca caagcgcatc gccgctctcc ggcaactggg gcgcgacacg gtggacgcca
      841 cggtatggcc gatgggtgct gtcgaagctt tattgctgga ccgatcgttg cgcttcagcc
      901 ggcaggaaag cgcgctggaa caaggctggc tgttggcgga gatggagcag cggttccgtt
      961 actcgttgga ggagttagcg cgccggttcg atcgcagcct ccgttgggtg gccaggcgtc
     1021 tggcgctggt cgagttatta ccggcgacga ttcagcagca agtccgcgag gggaagattg
     1081 cggcacagct ggcgatgaag tatctggtcc ccatcgcgcg cgtcagcgcg gaggattgcg
     1141 aacgaatggc cgccgtttgg gtgacgctgc actgcgacac ccggcaagcc gcacagcttt
     1201 atacggcgtg gcgcggagga tcacgcgtgg tgcgcgagcg cattctccaa gaaccggaac
     1261 tgttcctgag aacccaacgg ccatcggaga gggagactca gccggcggga gcggctctgg
     1321 agcgggatct ggagatggcc gtcgccctct tgcagcgtgt gagccgccgg ctctgcgccg
     1381 ccctgccgga gatgaatcgc cggcaacagg agcaaacgca acaccgcatc gaggacgccc
     1441 gccgggagct ccatcgcatc gccacacgaa tggaaaagga acaggagagc aaacatgctg
     1501 agccaggcgc aacggaccgc gattttggag atgaactcgc agcgcgtcag catccgcgag
     1561 acggcgcgcc tgttgaggtt gtcacgccag gtcatacgtc aagttctgcg ttcgaactcc
     1621 acgcttgtgc cagagattca cagagcggag aaggcagaac cgtaccggca acaggtcctc
     1681 gatcttttga gcacctgcaa gggcaatctc gtgcgagtcc atgaggaact aacggcgagc
     1741 ggcgcgttgc tgtcttatca ggcgctgacg gccttctgcc gtcggcacgg cctcggccaa
     1801 actcccagtg agccggcggg ccagtaccac tttgagcccg gcgtggagat gcagcatgat
     1861 acgtctccgc atgaagtgga agtgggtgga aaagcatata aggtgcagac cgcgtcggcg
     1921 gtgttgtgtt actcgcgctt gctgttcttc cagatcaatc ccacctttca gcgcttcgat
     1981 tgcaaagtgt tcctcacgga ggcgctgcgc tatatgggcg gcactcctaa acagatcatg
     2041 atcgacaaca ctcatgtcgt tgtgctgcgc ggaaccggcc gcgcgatgat cccagtgccc
     2101 gagatggagg cgttcgcgga acgctttgga ttcgattttg tggcgcatga acgcggcgac
     2161 gccaaccgct ccgggcgtgt cgagcggccg ttctccttta ttgagaataa ctttctcgct
     2221 ggacgctcgt tcagcagttg gaacgatctg aaccatcagg cacgccagtg gtgcgaccgt
     2281 gtgaatgcga cttacaagaa acatatccgc gccgtgcctc gcgaactgtt tgccgtcgag
     2341 cggttgcacc tgaaaccact gccggcttgg attccggagg tatatcgcct gcacgagcgc
     2401 acggtggatg tggaaggcta cgtctcgctg catgccacgc gttactcggt gccagtctcc
     2461 tggatcggac ggcgtgtgga aatccgcgag acgagcgaca aggtcgagat cgaactcgat
     2521 gcccgtcact tagtcacgca tgtccgtacg atggcgcctc tgcagcgcat cacactggcc
     2581 gaacacaagc cgccgcgtgg cgagcgagtg aagctgatcg agcgacatcc ggaggaacaa
     2641 gccatcctac acgcggcacc tgagattgcc ggctatgtgg cggaactgaa gcaaaagggc
     2701 cgtaaagtgg tggccttgtc tctgcggcaa ctgttacgaa tgcttcgcga atatccacgc
     2761 gagtccttcc tagccgcagt gcgcgaagcc gcccagtatg gcctctatga tctcgaccgg
     2821 ctggagcgga tgattctgcg gcgcgtggcg cgcgattact tcttgctcga tacagatacc
     2881 catgactgaa gaactcgaac aactgttgaa gaatctcaag ctgcggcgca tgctgcacgt
     2941 ctacgacgag caactgcagg cggccgagaa gtccaacccc tcctattcgg agttcattgc
     3001 cggattactg cgcgcacaat ggcacgagcg gcaggagagc gcgctggagt ggcgcattcg
     3061 caaagccaat ctgccggaac gctggtcact cgaaacgttc ccctacgcgc gccaacccgg
     3121 agtcaatcgt aaacagatcc gcgccttcgc cgaactcgac ttcgttgcca agcatgagaa
     3181 tctcgtcctg gtcggcccga ccggcgtggg caagaccgga ctagcgtgcg gactcctgtt
     3241 gaaagcccta cagaatggcc accgctgcca gttcatccgc gcgcaggatc tgttcgacga
     3301 aatgtacgcc tcgctcgccg accgctccac acgccagtta ctgaatcgcc tggcgcgcct
     3361 cgacgttttg ctaatcgacg agctagggta tttaaatctc aagccagaac aatcgaacac
     3421 cttcttcaag ctgatggagg aacgctatca tcgccactcc accatcatca ccactaactt
     3481 gatctatgac gagtggcata gcttcctcgg caataaatcg atggtcgatg ctctgttgag
     3541 ccgcgtgcga cattactgcc acaccgtgac cattaacggc ccctccttgc gcgatccgca
     3601 aggctgagtt ctgcttcatg gaacagcgcg agtatgtgcg ccgcctgctg gacgcctatc
     3661 gtgcgacgcc gggcacggca ggcgttgtcc gtcgtgcgga ccgtgtgttt gccgcccaac
     3721 tgcatgcgcg cgccgtaccg tttgaagtgg tggagaacgc tttgacgctg gccgccgccc
     3781 ggcgactggc tcgtcccacc gatgctccgc ccctcggcgt gattcgttcg ctggcctact
     3841 tctcgccggt cattgaagaa gtcctgcaaa cgcaaccgag cccggagtac ttccggcact
     3901 tgcgccagcg catggccaaa ctcacatccg cccgatagct gaggctttat ccaacagaag
     3961 gcacgcctcc ggcgtggatg atgttgccgg gcctgcgccc gtctctcaca ccagcgcgcc
     4021 ttcggcgcgg atgatgaccg tgctcccagc gcttttcgaa tcttgttctc agagcgggca
     4081 acttctatca agccctactg ggtaacttct cgtgagcgct gacgcgcccg gcagcccgaa
     4141 cccaccgcaa tggcccgcgc cgcgccgaac ttcggcagaa gacatcggca cctgcttacg
     4201 gcgaaggcgc ggctttgggg tgccgccgtg agtgcggcgg agcaaccctc atcgatttat
     4261 caggttacgc cacccttctg cccacagaag cgaatggcca atcgaattca aattcgcgtg
     4321 tgatggctga acgcatatca gtatcggcat tcatgagtga atcgctatga aggtacggaa
     4381 acggctcaca cgacaggaga gcaaagaggt gacgcgcacg cgtctcatcg aagctgccga
     4441 gagactattc atccggaaag gtttcgacga tgcctcggta gacgaaatca gcgaaacggc
     4501 cgggtactcc
//
LOCUS       DATMGX010000564         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_44543, whole genome shotgun sequence.
ACCESSION   DATMGX010000564 DATMGX010000000
VERSION     DATMGX010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44543"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..>2287
                     /locus_tag="VNR65_09205"
     CDS             116..>2287
                     /locus_tag="VNR65_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWJ18888.1"
                     /translation="MIGQTISHYRVIEKVGGGGMGVVYKAEDTRLHRFVALKFLPDDV
                     ARDPQALSRFQREAQAASALNHPNICTIYDIGQENGQAFIAMEYLEGATLKHMIEGRP
                     LKIAQLLELGIQISDALDAAHSGGIVHRDIKPANIFVTQRGQAKVLDFGLAKLTPQGS
                     HGVDGSAATVGAPTAVSADQLTTPGTAMGTIGYMSPEQARGETLDNRTDLFSFGVVLY
                     EMATGRQPFSGATSAVVFEAILNKIPAPPVQLNPGLPAQMEIILNKALEKDREMRCQS
                     AAELRADLRRLKRDTDSSRTGVRGSAPAAAPSGTKDLPVEVQESVRKGSRLLPVLIFC
                     GFLVALAAGLAAGKRLWGTSTASAPLYHEITFRRGEIRSARFAPDGQTILYSAAWQGN
                     PAEIFAARQGTVESRSLGLGRAELLAVSSTGEMALSLDSHPAGTWVNVGTLARAPLAG
                     GAPRPILEDVEWADWAPDGNSLAVVHNVRGRDRLEYPIGKVLYETSGGWISYPRVSPK
                     GDYVAFVDHPNQGDDGGSVSVVDLSGKKKKLTRDWYGTGGLAWSPNGQEVWFTASELG
                     VDHYLSAVSMEGKERLVTRIPGTLVVFDIWHDGRVLLARAGRRREVMALYDGSPKERD
                     LSWLDYSYPADLSPDGKTVLFDEEGVGGGVQYGDAQELTYAVYIRNTDGSPAIRLGEG
                     VADALSPDQKWVIVQSPGSPEQLRLLPTGAGETRTLTNDSIN"
BASE COUNT          457 a          685 c          707 g          438 t
ORIGIN      
        1 cgctgagtcc ggggcatcgt tgattgtgta gcgacgtgaa ccccagcttc gcactccatt
       61 gacttgccgt tagctcggcc cctaatatgg tcgctcacgg ggcggtcctt cttcattgat
      121 cggacagacc atatcgcact accgcgtcat tgaaaaagtt ggcggcggag gcatgggcgt
      181 cgtgtataag gccgaggaca cccgactgca ccgctttgtc gcgctcaagt tcctccccga
      241 tgacgtggct agagatcctc aagctcttag ccgctttcag cgtgaggcac aggctgcctc
      301 cgccctcaac catccgaaca tctgcaccat ctacgacatc ggccaggaga acgggcaggc
      361 tttcattgcc atggagtatc tggaaggcgc gaccctcaag cacatgatcg agggccgtcc
      421 gctcaagatc gcgcaactgc tcgaattggg gatccagatc tccgatgctc ttgatgcggc
      481 tcactcgggc ggaattgtgc accgcgacat caagcctgcg aatatcttcg tcacgcaaag
      541 aggtcaggcc aaggtgctcg attttggctt ggcgaaactt actccgcaag ggagccacgg
      601 cgtggatgga tcggccgcca ccgtgggggc tccgaccgcc gtcagcgccg accaactaac
      661 tactccggga acggcgatgg gaaccatcgg ctacatgtca cccgaacaag cgcgcggcga
      721 gacgctggac aatcgcaccg atctgttttc tttcggtgtg gtgctctacg aaatggcaac
      781 cggccggcag ccattctcgg gagctacatc tgctgtcgtc tttgaggcga ttctgaacaa
      841 gatacccgct ccaccggtcc agctgaatcc gggattgccg gcacagatgg aaattatcct
      901 gaacaaggcg ctggaaaaag atcgggagat gcgctgccaa agcgcggcgg agcttcgcgc
      961 cgacctgagg cggctgaagc gcgacaccga ttcttcccgc actggggtta gaggtagcgc
     1021 cccggcagcg gcgccgagcg gaacgaaaga ccttcctgtc gaagtgcagg aaagtgtgcg
     1081 aaaaggtagc cgcctcctgc cggtgctgat tttctgcggc ttcctggttg ctttggcagc
     1141 cggattagca gcgggcaagc gtctctgggg aacttctacg gcaagtgcgc cgctgtatca
     1201 cgagatcact ttccgccggg gcgagattcg ctccgcccga ttcgcaccgg acgggcagac
     1261 gattttatac agcgccgcct ggcaaggaaa cccagccgaa atattcgccg cgcgccaggg
     1321 cactgtcgaa tcgcgttctc tcggactggg ccgcgccgag ctgctggccg tttcatcgac
     1381 gggtgagatg gcgctttctc tggacagcca cccagcggga acctgggtaa acgtgggcac
     1441 gctggcgagg gcgccattgg caggcggcgc tccccgcccg attctcgagg atgtcgaatg
     1501 ggccgattgg gccccggacg gaaacagctt agccgtcgta cacaacgtac gtggccgcga
     1561 ccgcttggaa tatcccattg gcaaagttct ctacgagacc agcggcggct ggatcagtta
     1621 tccgcgagtc tcccccaagg gcgactacgt ggccttcgtt gaccatccca accagggaga
     1681 cgacggcggc tcggtgtccg tcgttgatct ctccggcaag aagaaaaagc tgacgcgtga
     1741 ctggtatggt acggggggcc ttgcctggtc acctaacggc caggaagtct ggttcacggc
     1801 cagcgaactg ggagtggatc actatctctc cgccgtcagt atggagggga aagaacggct
     1861 ggtgacgcgg atacccggca cgttggttgt gttcgacatc tggcacgatg gacgcgtgct
     1921 gctggcgcgt gccggccggc ggcgcgaggt catggcgctt tacgacggca gccccaaaga
     1981 gcgagacctt tcatggcttg actactccta tcccgccgac ctttcgcccg atggcaagac
     2041 cgttcttttt gacgaggagg gagttggcgg cggagtgcag tacggggatg cacaagaact
     2101 aacctacgcc gtttacattc ggaacaccga tggctccccg gcaattcgac tcggcgaagg
     2161 ggtcgccgat gctctgtctc ccgatcaaaa gtgggtcatc gtgcagagtc cgggctcgcc
     2221 cgagcaactc cggctgctac caaccggcgc gggggaaacc aggacgctta ccaacgattc
     2281 gattaac
//
LOCUS       DATMGX010000565         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_33763, whole genome shotgun sequence.
ACCESSION   DATMGX010000565 DATMGX010000000
VERSION     DATMGX010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33763"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..>2080)
                     /locus_tag="VNR65_09210"
     CDS             complement(206..>2080)
                     /locus_tag="VNR65_09210"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWJ18889.1"
                     /translation="EEPTTSSPADQSRPEKPASAMSASSQDPEDAAADKQPAGQAPAT
                     TESEAERPGEGEAPAPAAEEAAEAKPEVEPSPTVEPATKPSIALQPAEPAVPSKAASE
                     PSPKTLNVPASTSAEAEALRKALSSLSAGASDEEQNEHAALKSFYEARGFAPLWTAPQ
                     TGFTPAASAVAAEIKRARDWGLDPRDFALPAGLGADGAAAQTPEGMAAAEVKLSFAIL
                     KYGRYARGGRIINPSEQLSSYLDRRPQLLKPQAILDGIATSDQPDAYLHSLNPKHPQF
                     ERLRQKYLALATHSKKQSGEAKRLLANMEEWRWMPEDMGDLYVWNNIPEFTQRVMKDG
                     EVARKEAIVTGQIGKETPIFSRPLRKITFKPTWIVPDSIKVRELLPDLMRGGGLMRQW
                     GLEVLRDGQPVNWRKIDWAATDIRNFEVIQPNGPKSVMGKVKFSFPSQHTVFMHDAAE
                     GEKWMFRSPKRTYSHGCMRVQNPVGLAEILLREDKGWDVAKVREILNTGGNNNEIPIE
                     RKIMVHMTYFTALVDDDGKLHTFPDVYGHERRITLALEGKWNQIEKGRDHLAPVELNE
                     ASTVRPKHYAEDEPYDSRVWRRDSPPSSNGSFLDSLFGGSRSEYGQYGQYPAPPRQYR
                     "
BASE COUNT          354 a          623 c          643 g          460 t
ORIGIN      
        1 agcccggcca tgacatcggt tccagctaat cgcaacacgc tttaaggaac gggggcggcc
       61 tcaaaagcac cacctacata ggaactcgga gccacgagcg accattcgac cggaattgag
      121 gcaagacaga ccccttcatt acagccgcag ctactgaagg cgatccaaaa aagcccctgt
      181 tcgttggcga agccgaatgt ttgcttcagc ggtattgacg cgggggggcg ggatattgcc
      241 cgtactgtcc atattcgctc ctagatccgc cgaaaagcga gtcaaggaaa ctcccgttgg
      301 aactgggcgg gctgtcgcgg cgccagaccc gcgagtcata gggttcgtct tcggcgtaat
      361 gtttcggacg cacagtgctg gcttcgttga gttccaccgg cgccaggtga tcgcgtccct
      421 tttcgatctg gttccacttg ccttccagcg cgagcgtgat gcggcgctcg tggccgtaga
      481 cgtcgggaaa tgtgtgcagc ttgccgtcgt cgtccaccag agccgtaaag taggtcatgt
      541 gaaccatgat cttcctctcg atcgggatct cgttgttgtt gccgcccgtg ttgaggattt
      601 ccctgacctt ggcgacgtcc cagcccttgt cctcgcgaag caggatttca gcgagcccaa
      661 cggggttctg gacccgcata cagccatggc tgtaggtgcg cttgggcgag cggaacatcc
      721 atttttctcc ctcggccgca tcgtgcatga aaacggtgtg ctggctgggg aatgaaaatt
      781 tcactttccc catgacgctc ttcggcccgt ttggctgaat gacctcgaaa tttcgaatat
      841 ccgtggccgc ccaatcgatc ttgcgccaat tgaccggctg gccgtccctg agcacctcga
      901 gcccccattg gcgcatcaag ccgccgcccc gcatcaggtc gggcaacagt tcacgcactt
      961 tgatggagtc gggcacaatc catgtcggct tgaaggtgat tttgcgcagt ggccggctga
     1021 agataggcgt ctccttgccg atttggccag tgacgatggc ctccttgcgc gcgacctcgc
     1081 cgtccttcat cacccgctgc gtgaattccg ggatgttatt ccagacatag agatcgccca
     1141 tgtcttcggg catccagcgc cactcttcca tgttggcgag cagccttttg gcttcgccac
     1201 tttgcttctt gctgtgggtc gcaagcgcca gatatttctg ccggagcctc tcaaactgcg
     1261 ggtgctttgg attgaggctg tgcagatagg catcaggctg atcggaagtc gcgatgccat
     1321 caaggatggc ttgcggcttg agaagttgag gtcgtcgatc gagataggaa ctcagctgct
     1381 cggagggatt gatgatgcgc ccgccgcggg cgtagcggcc atatttcagg atcgcgaacg
     1441 aaagtttgac ctccgcggcg gccatccctt caggcgtctg ggcggctgcg ccatcggccc
     1501 ctaaccccgc aggcagggca aaatcgcgcg ggtcgagccc ccagtccctg gcccgtttga
     1561 tctcggcggc aactgcagat gccgcaggcg tgaagcccgt ctgcggtgcc gtccagagcg
     1621 gagcaaatcc gcgggcctca taaaacgact taagcgcagc atgttcgttt tgttcttcgt
     1681 cgctggctcc cgccgaaaga ctggaaagcg ctttgcgcag ggcctccgcc tcggcagatg
     1741 tggaagccgg cacgttcaat gtcttcggcg acggctctga tgccgccttt gatggaacgg
     1801 cgggctcagc cggctgcagg gctatgctcg gtttcgtggc gggctcaacc gtgggcgatg
     1861 gctcgacttc cggcttggct tcggcggctt cctcggctgc tggcgcaggc gcttctcctt
     1921 cgccggggcg ctcggcctcg gactcggtgg ttgctggagc ctgtccggct ggttgtttgt
     1981 ccgccgccgc gtcctcaggg tcttgagaag aggcgctcat cgccgaagcg ggcttctcgg
     2041 ggcgcgactg atcggctggc gatgaggtgg ttggctcctc
//
LOCUS       DATMGX010000566         3675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_176594, whole genome shotgun sequence.
ACCESSION   DATMGX010000566 DATMGX010000000
VERSION     DATMGX010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176594"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VNR65_09215"
     CDS             complement(<1..411)
                     /locus_tag="VNR65_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWJ18890.1"
                     /translation="MTYDYVIVGGGSAGCVLANRLSADPSVNVALLEAGPGDKNFLIH
                     MPMGYAALMKTGWVDWGYHTEPQAGMGGRRLYWPRGKVLGGSSSVNAMIYIRGVPSDY
                     ETWAQLGNRGWAWDNVLPYFKKAETYWYGTDRYHG"
     gene            708..>3675
                     /locus_tag="VNR65_09220"
     CDS             708..>3675
                     /locus_tag="VNR65_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWJ18891.1"
                     /translation="MMDGWVIITCSLLYIGALFGIAYLGDRTGKKAPAPNGRPYVYAL
                     ALGVYCTSWTFFGSVGSASEKGFDFIAVYLGPIIAFTLALPVLRRITRLTKDQNITSI
                     ADFIAARYGKNPTVAAIVTIVAVVGVLPYIALQLKAISQAIAVLLGHPTQGASAVMLP
                     IGDLDLVIALVLATFTILFGARHSDATEHQRGLMLAVAVESIVKLCAFILVGIYVVYV
                     MNDGPSNLIATAMTQTDAYLFNTGIHGGTLLTVTFLSFVCIILLPRQFHVTFVENVSK
                     EELNKASWLFPLYLIAINIFVVPVAMAGSLMLGNAYPHDMYLLALPLHAKSQIFTFIA
                     FLGGLSAATAMVVVETVALSVMLCNDLLIPLILKRPSMAAAGRRPDISGFILNVRRAA
                     MLSIILAAYAFYDKIGNASLSEIGLLSFAAIAQFAPAFFIGLFWRRATARGAAAGILA
                     GFAVWAYTLLLPKLIADTAAGAAFLNDGPFGIAFLRPEMLFYLQFDHLTHGVVWSIAL
                     NLVAFLSVSLLRPLEPIERLQTNLFIEDANLHLLPAFKPWRTSVPFRDLMSTVVRYLG
                     EERTRRSFADYMASRGIDPSSPGQADPHAVKFTENLLASAIGAPSSRLVMSLMLRRFN
                     VGRDSALRLLDEASEAVQYNRDLLQCALDHVGQGIAVFDKKMQLICWNRQYREILMLP
                     PQFARVGTPLDEIIRFKAKRVHKDPTAVEEFVTDRIVKYQLTEQGFHECTMDNRVIEV
                     RVNAMPQGGVVVTYTDITERQAAAEALARANENLERRVEARTLELAIAKAKADEANIS
                     KTRFLAAASHDVLQPLNAARLYASSLLERSPPAELGRLARNIDASLDGVEEILNALLD
                     ISRLDSGAMKPEFGVFPVKDLLEQITIDFEPIAKARGISFIIVPSSALIRSDRKLLRR
                     ILQNLVSNALKYNKDKGRALLGCRRRGRNLIIEVHDSGPGIADDQRDVIFKEFQRLDH
                     HSHAPGLGLGLSIVERM"
BASE COUNT          715 a         1123 c         1004 g          833 t
ORIGIN      
        1 gccgtgatag cggtctgtgc cgtaccagta ggtttcggct ttcttgaaat agggcaggac
       61 attgtcccag gcccagcccc ggttcccaag ttgtgcccac gtttcgtaat cgcttggaac
      121 gccgcggata taaatcatgg cgttgaccga gcttgatccg ccaagaacct tcccgcgcgg
      181 ccagtacagc cgccgcccgc ccattcctgc ctgcggttca gtgtgatagc cccagtcgac
      241 ccagccggtt ttcatgagcg ccgcgtagcc catcggcatg tggataagga aattcttatc
      301 gccgggtccg gcctcaagga gcgccacgtt gacggagggg tcggctgaaa ggcggttcgc
      361 gaggacacag ccagctgaac cgccgcctac aatgacgtaa tcgtaagtca tggcaactcc
      421 tcccgccccg tccgcgatct atcttatttg ggtagagcct gagctgtaag aagggtatgc
      481 ctttacccgc ttgaaacggt tgggccaaag gaacacctaa ccttccaagg acgaaattta
      541 attgcgcgta aaaggataat tttatattcg tcgaatagat ttgcacttgc atgccaaggg
      601 cgcgcgttgc ttaattatgg tataagattt tagaatatga tggacgcgtg gaacaacagg
      661 ccgtcatcgc atgacgcttc ggtcaaaagc ctcggcatga gcgcgagatg atggacggct
      721 gggtcataat tacctgttcc ctcctgtata tcggagcgct tttcggcatt gcctatcttg
      781 gcgatcgcac cggcaagaaa gccccagcgc cgaatggacg cccctacgtc tatgctcttg
      841 ctttgggggt ctactgcacg tcctggacgt tcttcggaag cgtgggatcg gcctcggaaa
      901 aggggttcga tttcattgcg gtctatctgg ggcccatcat cgcttttacg ctagccctgc
      961 ccgtgctgcg ccgcataaca cggctgacga aagatcagaa tattacgtcg attgccgatt
     1021 tcatcgcggc gcgctatggc aaaaatccca ccgtagcggc gatcgtaacc atcgtggcgg
     1081 tggtcggcgt attgccctat attgcccttc agctgaaagc catctcacaa gcaattgctg
     1141 ttctgctggg gcatccgacg caaggtgcca gcgccgtcat gctgccgatc ggcgatttgg
     1201 atttggtcat agcgctcgtc ctggctacgt ttacaatcct cttcggcgcg cggcacagcg
     1261 atgcaacgga acatcagcgc gggctcatgc ttgcggttgc cgtcgagtcg atcgtcaagc
     1321 tttgcgcctt tatcctggtc ggcatctatg tcgtctacgt catgaacgat ggtccgagta
     1381 atctgatcgc aacggccatg acgcagacgg acgcctatct cttcaatacc gggatccacg
     1441 gtggcaccct tctgaccgtc acgttcctca gcttcgtctg tataatcctg cttccccggc
     1501 agtttcacgt aacgttcgtc gagaatgtct ccaaggaaga gctaaacaag gcctcctggc
     1561 ttttccccct ctatctgatc gcaatcaata tctttgtcgt gcctgtggcg atggccggga
     1621 gcctcatgct tgggaatgca tacccgcacg acatgtacct ccttgcgctc ccgctgcacg
     1681 caaaaagcca gattttcaca ttcattgcct ttctgggggg cctgtccgcc gcgacggcga
     1741 tggtggtagt tgagacggtc gcgctgtccg ttatgctctg caacgacctt ctgatacccc
     1801 ttatcctgaa gcggcccagc atggccgctg cggggcgtcg gccagacatt tccggcttta
     1861 tcttgaacgt gcgtcgcgcg gcgatgcttt ccatcatcct ggccgcctac gccttctacg
     1921 ataagatcgg gaatgcttcg ctctcggaaa tcgggcttct ctccttcgca gccatcgccc
     1981 aattcgctcc tgcctttttc atcggtctct tctggcgcag agccacggca aggggcgctg
     2041 cggctggcat tctcgccggt tttgccgtct gggcgtacac gcttcttctt ccgaaactga
     2101 tagcggatac cgcggcgggt gcagcgtttc taaatgatgg cccattcggc atcgcttttc
     2161 tgcgcccgga aatgcttttc tacctgcagt tcgaccacct cacacacggc gtggtttgga
     2221 gcatcgcatt aaaccttgtg gcgtttctct ccgtatctct gcttcgtcct cttgagccga
     2281 tcgagcggct ccaaaccaat ctcttcatcg aggatgccaa cctccatctg ttgccggcct
     2341 tcaaaccctg gcgcaccagc gttcccttta gagatctcat gtcgacggtc gtgcgctatc
     2401 tcggcgagga gagaacccga cggtcctttg cagactacat ggccagccgt ggcatagacc
     2461 cgtcctcgcc tggacaagcc gacccgcacg ccgtcaagtt cacggaaaac ctgttggcca
     2521 gcgcaatcgg cgcgccgtcc tcccggcttg tcatgtcgct catgctgcgg cgtttcaatg
     2581 tcggccgcga ttcggccttg aggcttctcg atgaggcttc ggaggcggtg caatacaatc
     2641 gcgatctgtt gcagtgcgcc cttgaccacg ttggccaagg tatcgctgtc ttcgacaaga
     2701 agatgcagct catctgctgg aaccgccagt accgcgaaat cctgatgctg ccaccccagt
     2761 tcgcacgcgt aggaacaccg ctcgacgaga ttatccgctt caaggcgaag cgggtccaca
     2821 aggaccccac cgccgttgaa gagttcgtca ctgatcggat cgtgaaatac cagctcacgg
     2881 agcagggctt tcacgagtgc accatggata accgcgtgat cgaagtgcgg gtcaacgcca
     2941 tgccacaggg cggcgtcgtt gtgacctata cggacatcac cgagcgccag gccgccgccg
     3001 aggctcttgc acgagccaac gagaacctgg aacggcgcgt ggaggcccgg actttggaac
     3061 tggccatcgc caaggcgaaa gccgatgaag ccaacatcag caaaacccgc tttctcgcgg
     3121 cagcgagtca tgacgttctt cagccgttga acgcggcccg cctttacgcc tcgagcctgt
     3181 tggaacgctc gcctccggcc gagctcggcc gcctcgcccg caatatcgat gcctcgctcg
     3241 acggcgtcga ggaaatcctg aacgcgcttc tcgacatctc ccggctcgac agcggcgcga
     3301 tgaagccgga attcggcgtg ttcccggtga aggacttgct cgaacagatt accatcgact
     3361 tcgaaccgat cgccaaggca cgcgggatct ctttcatcat cgtgccgtcg tctgccctga
     3421 tccggtccga ccgaaagctt ttgcgacgca ttcttcagaa ccttgtctcg aacgcgctca
     3481 aatataacaa ggacaaaggc cgcgccttgc ttgggtgccg ccggcgcggg cgcaatctga
     3541 tcatagaggt tcacgacagc ggaccgggaa tagctgacga ccagcgtgac gtgatcttca
     3601 aggagttcca gcggctcgat catcatagcc acgcccctgg cctggggctt ggcctttcca
     3661 ttgtcgaacg catgt
//
LOCUS       DATMGX010000567         2822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_255140, whole genome shotgun sequence.
ACCESSION   DATMGX010000567 DATMGX010000000
VERSION     DATMGX010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255140"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(553..1929)
                     /locus_tag="VNR65_09225"
     CDS             complement(553..1929)
                     /locus_tag="VNR65_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ18892.1"
                     /translation="MDNETLQALIKVYKARASTDLLSIIMDEAEGPQARAAVLELLHS
                     LPPDSLADNEFAPVRRKACHFCLGAEDYDLADRIARATELPEDRVLRARALHGLNREQ
                     EAAVLYRQAITQDPAIRNRDIERLLGIRPSPNISQAPAKIIPLNTYSSRRDNKAEQDR
                     RDLAADSFLDDFEDVAVTFGDVVGLDDIKAEIRRRIVLPYLKPTLFDRYKQKAGGNIL
                     LYGPPGCGKTLIARATAGESDARFLSVNPEDIIDRYTGEAEKRLHVLFDEARSDTPSI
                     LFFDDFDVLAFRRAGLPPEAAPALIAAFASELDGTQRNNGGVLTIMATNAPWALDTAL
                     LRAGRFQRLLFVPPPGLEARKRILMEAVSNVPGADRVAFDRIARRSAGLSGGDLRALA
                     DWVSNTSLTKALANASDAGVTTALFEEGLKLFGPSTRNWLSLAKTEMKPIQRHEALGR
                     LFFPLYRA"
     gene            complement(2207..>2822)
                     /locus_tag="VNR65_09230"
     CDS             complement(2207..>2822)
                     /locus_tag="VNR65_09230"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWJ18893.1"
                     /translation="LKLLHPFMPFVTEELWAVLGENGPKRESLLCLVEWPKADKKLDQ
                     KPVAEVNWVIDLISEIRSLRSEMNVPPASLLPLTITGADEETKARAECYESFIKRMAR
                     LSDIAFADTPPQGSAQFVLGQATMALALANVIDLAAERERLSKEIGKLQKEIDKIDER
                     FANEQFMAKAPEEVVEENRERRAEAEAAAKKLKAALERLQAVAA"
BASE COUNT          580 a          842 c          816 g          584 t
ORIGIN      
        1 ctcataccag ccgtcagagc atccaactcc taccgtccat tgcctagagt gcgtccggaa
       61 aagcgcgaag cggttttccg ataaagacgc acgacataac aaagacttag agcccgcttt
      121 taattccatc aaaagcggat aggctctaga gactaatccc ctatgcgcga ggccgctttg
      181 gtgacgtgac cacagccaac agtctcggaa acttttgtcg caccgagccc cattaaaatg
      241 gcgacttggc gatcgcgact tatgcagctt ccaaaggtcc aagacagcac cctccaccgg
      301 aaacattccc ggtatcgtcg aagatacgaa tttttcgtcg gggaagaagc gccagcatga
      361 caaccggcgg cttcggtggc ataagccact tataaaattt gccggaaact gtttttgttg
      421 ccgacgaagg gatggtgttc cagttttatg gcctaaacgg ggcaaggatg ccgcagtccg
      481 tgctatttca gcaacagacg aactgtaagc ggcaaagctg caggcgcgac cgcgtctagc
      541 ggcgcgcagg tttcaggcgc ggtaaagtgg gaagaagagt cggccgaggg cttcgtgccg
      601 ttggatcggc ttcatctccg ttttggcgag gctgagccaa ttgcgtgtag aagggccgaa
      661 caacttgagg ccctcctcga aaagcgcggt tgtgacgccg gcgtcggaag cattcgcgag
      721 agcttttgtg agcgaggtgt tgctgaccca atccgcaaga gcgcgcaggt caccgcctga
      781 caagccggcc gacctccggg cgatccggtc aaatgcgacc ctgtccgcac ctggaacgtt
      841 cgataccgct tccatcagga ttcgttttcg tgcttcgagc cccggggggg gcacgaaaag
      901 caggcgctga aaccgaccag cccttaacag ggcggtgtcc aaagcccacg gggcattcgt
      961 cgccatgatg gtcaggacgc cgccattatt gcgctgcgtg ccatccaact cggacgcgaa
     1021 agcagcaatc aaggctgggg ctgcctcagg tggcaggccc gcgcgccgaa acgcgagcac
     1081 atcgaaatca tcgaaaaata ggatggatgg cgtatccgac cgcgcctcgt caaaaagcac
     1141 gtgcagccgc ttttccgcct cccccgtata tctgtcgata atgtcttccg gattgacgct
     1201 cagaaagcgt gcgtcactct cgcccgccgt ggctctcgca atgagagttt tgccgcagcc
     1261 gggtggcccg tacaggagga tattgccgcc agccttctgc ttgtaccggt caaagagggt
     1321 gggcttgagg tagggcagca cgatgcggcg gcgaatctct gccttgatgt cgtcgagacc
     1381 gacgacatca ccgaaagtca cggccacgtc ttcgaaatca tcgagaaaac tgtcggcagc
     1441 taagtcgcgc cggtcctgct cagccttgtt gtcgcgcctg gaggagtatg tattgagcgg
     1501 gatgatcttt gccggagcct gggaaatgtt cggacttgga cggatgccga gaagacgctc
     1561 aatgtcacgg ttgcgaatcg ccgggtcctg cgtgatcgcc tgccggtaga gcacggcggc
     1621 ttcttgctcg cggttcaatc cgtgcagcgc gcgtgcccta agaacgcggt cctccggaag
     1681 ttccgttgcg cgggctatgc ggtccgccag atcgtaatct tctgcgccga ggcaaaagtg
     1741 gcaagctttg cgacgaacgg gcgcgaactc gttatccgca agggaatcag gcgggaggga
     1801 gtgcaacaac tccagcaccg cggcccgcgc ctgcggccct tctgcctcgt ccatgatgat
     1861 ggataggaga tcggtgcttg cgcgcgcctt gtacaccttg atcagcgctt gcagcgtctc
     1921 gttatccata tgcgcggagc ccctcaccgt gagggcacac ccgcgatatg ccgcgtaatg
     1981 agcgccatta ccgcttcccc gtccattgtg cgccagataa caaatgcccc aaacccaaaa
     2041 cgcaagagca tcaattcgaa cgcaagggcg agttgggaga tttccgcctc tttgtggcgc
     2101 ctacaccaca ataactctgc ggagttactc gcccctcgct gaaattgcat ttcttttcat
     2161 gaaagtgtca cgttcaaggc ggcgatccgg ataaattagc gctgactcat gcggccacag
     2221 cttgcagccg ttcaagcgcg gccttcagct ttttggccgc agcttctgct tccgctctgc
     2281 gctctcgatt ctcctcgacg acctcttcgg gagccttcgc catgaactgc tcattggcga
     2341 atcgctcgtc gatcttgtcg atttcctttt gcagcttgcc gatctctttc gagagacgct
     2401 cccgctcggc ggcaagatcg ataacatttg ccaaggccaa tgccatcgtc gcctggccga
     2461 gcacgaattg cgccgaccct tgcggcggcg tatcggcgaa ggcgatgtcg ctcagccttg
     2521 ccatgcgctt gatgaagctc tcatagcatt ccgcgcgggc cttcgtctcc tcatcggcgc
     2581 cggtgatcgt gagggggaga agtgacgctg gcggcacatt catctcggag cgcagcgacc
     2641 ggatttcgct gatcaggtcg atgacccaat tcacctccgc gaccggcttc tggtccaatt
     2701 tcttgtcggc cttcggccac tccaccaggc agagcaggga ttcgcgcttg ggaccgttct
     2761 cccccagcac cgcccacagt tcctcggtga cgaacggcat gaacggatgg aggagcttca
     2821 gg
//
LOCUS       DATMGX010000568         4375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_32719, whole genome shotgun sequence.
ACCESSION   DATMGX010000568 DATMGX010000000
VERSION     DATMGX010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32719"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VNR65_09235"
     CDS             complement(<1..167)
                     /locus_tag="VNR65_09235"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWJ18894.1"
                     /translation="MIHFPVPEALTFDDVLLLPAKSDVVPALVETTTRLTRNIELRIP
                     LISAAMDTVTES"
     gene            565..1062
                     /gene="rimP"
                     /locus_tag="VNR65_09240"
     CDS             565..1062
                     /gene="rimP"
                     /locus_tag="VNR65_09240"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWJ18895.1"
                     /translation="MAASLEKVREIAERVAQSSGLEVVDIELLGGGKVRMLRIFIDKP
                     DGVTHEDCSSYSREVGTIFDVEEAVPGTSYLLEVSSPGLDRKLSKPADYQRFTGSLVK
                     LMTRDPVNGNRHFEGRLKAFHDGRLSLELTGRKKSKKHQEVEAGTEVEIELANVEKAN
                     LVPEF"
     gene            1109..2752
                     /gene="nusA"
                     /locus_tag="VNR65_09245"
     CDS             1109..2752
                     /gene="nusA"
                     /locus_tag="VNR65_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWJ18896.1"
                     /translation="MASELYNVIDALSREKGIDPQIVVNAVEDAIVVATRKYYKTQEN
                     LRAELDKESGQIRAFAVRTIVDGPDQLEDPLAQITLEDAKRIDPAAEVGGELRTYKPT
                     DVLGRIAAQLAKQVIFQKVREAERDTVYNEYIGRVGEVINATIKRQEGQDVIVDLGKA
                     EGRMPKKEQSRLESFAPGERARVVIVRVEKASKGPQVVVSRAVPELVSHLFQTEVPEI
                     YDNTVVIRAIAREAGERTKIAVMSKDKDVDPVGACVGMKGMRVQSIIRELRGEKIDII
                     EYHEDSVVFAEKALQPAKVSRVTILEGQEKHLEVIVDDSQLSLAIGKKGQNVRLAAKL
                     LGWKIDIKSEEEKRQEVEDRMNIVLNQPITPLESVPDLEPGIIEKLVAAGITTVEAVA
                     DMTPEQLEEVPGIGPKTVEKISIAVNNYFSSLDAAANAGSEEVPLSEGEVSAEEVATE
                     DEPVTEDEAPTEGGGDDSATGPDSGGQSGDVAGLDEEPEAGPDSVEELVETGQYYEAE
                     VVEGIEDAPLADEAEVTTHERPAGENENVSDEEKPGEQK"
     gene            2853..>4375
                     /locus_tag="VNR65_09250"
     CDS             2853..>4375
                     /locus_tag="VNR65_09250"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HWJ18897.1"
                     /translation="MQKIRINDLARELEVKSKAILDALIKVGVTEKKTHSSSIEVDEA
                     ERVKKYFAGHAEPGTSRASRAPADEFKPKLDLSHISKPGDVLKAVTQRAAQPSAVARP
                     ATPPTHATAAGPRQTVAPPSAVRPAPPVAKPVTSVASATPAAPAEPPKQAPRFITPQS
                     VARPPAAIVIPPKPPVAAPPATPPAHLPSATGTALPSEPVIEDQITALEESEVLPSTS
                     EAIESEIPTAVLREVPPALLEAQPPATENRAVTGRPVAPGQSAQGIGRPGLPTRPGQP
                     GIGATAAHAPRRLIVPQTGPRPVYLAPPVAPPRPGAPIAPRGGMPERGKPIFQRPRPG
                     VPPQGQTRPPVRPGERRGPHPTRSGAPGARPGFGAGAGAAPPPPTGQRPAGRPARPGQ
                     RYVPRGVKEGPMKGFVPPPRLSLSSEPLPITRSITIPEGISVKDLAEKLGIRAKDLIA
                     RLLARGVFATVNQTLDSDLATDMARHFGAETAVITFEEQLAKDTVPAEGAGEEGAALG
                     "
BASE COUNT          969 a         1239 c         1405 g          762 t
ORIGIN      
        1 gactccgtga ccgtgtccat ggccgcgctg atgagcggga tgcgaagttc gatgttgcgg
       61 gtcaggcggg tcgtcgtctc caccaaggcc ggaacgacat cggacttggc aggcaacagc
      121 aacacgtcat cgaaggtaag cgcctcaggc acaggaaaat gaatcatggc atttggtccg
      181 cggagagata atctctcatt gtagagttgc gctggcggtt aggcaaacgc gaacacagca
      241 ggattgttgg gctgccatga ggagagatac ccgcgctttg gtgtgctccg ctttgggccg
      301 ctattgcccg gttggtttat tgaattttcg gactctttga cgtatagtta gtgaggtact
      361 gttgtgtttt gagggttctg tcaccccctc ccggcgtgac ggcgagcccc gtgcataatc
      421 ggcgaagcac atcggtgggc gagagcccac tttttatttc caccccgtaa tccgctggtg
      481 cacggcgagt ccaggcggca gcgagagcct gacgggcggt ttgtgctcat tcgcagggag
      541 cagttttttg gtcaagaaca gacgatggca gccagtttgg aaaaagttcg agagatcgca
      601 gagcgggtag cgcaatcgag cggcctggaa gtggtcgata ttgagctgct gggcggcggt
      661 aaagtccgca tgctccgcat cttcatcgac aagccggatg gagttacgca cgaggattgt
      721 tccagctaca gccgcgaagt gggcacgatc ttcgatgttg aagaggctgt accgggcact
      781 tcgtacttgt tggaggtttc atcgcctgga cttgatcgca agctttcaaa gccggcggac
      841 taccaacgtt tcacgggcag tctggtgaag ctgatgaccc gggacccggt gaatggaaac
      901 cggcattttg aaggccgact gaaggcgttt cacgatggcc ggctcagttt ggaattgact
      961 ggcaggaaaa agtcgaagaa gcaccaggaa gtggaagcgg gaacggaagt tgaaatcgag
     1021 ttggccaacg tcgagaaggc aaacctggtt cccgagtttt gattcggacg cagtaaagac
     1081 caaatggaag taaggtggcg aacattttat ggcgagtgaa ctgtacaacg taatcgacgc
     1141 tctgagccgg gaaaaaggca tcgatccgca gatcgtcgtg aacgcggtgg aggatgccat
     1201 cgttgtggcc acccgaaagt attacaagac gcaggagaac ctgcgcgccg agctcgacaa
     1261 agagagcggc cagattcgcg cgttcgcggt acgaacgatc gtcgatggcc ccgaccagct
     1321 cgaggatccc ctggcgcaga ttacgctgga ggacgccaaa cgcatcgatc ctgccgcgga
     1381 ggtgggtggc gagttgcgca cctataaacc caccgacgtg ttaggccgca ttgcggcgca
     1441 actggcgaag caggtcatct ttcagaaggt ccgggaggcc gaacgagata cggtctacaa
     1501 cgagtacatc gggcgtgtcg gtgaagtgat caacgccacc atcaaacgcc aggagggcca
     1561 ggacgtcatt gtggacctcg gcaaggccga agggcgcatg cctaagaagg agcaatcgcg
     1621 cctggaatcg ttcgctcccg gcgagcgtgc ccgagtggtg atcgtccgcg tggagaaagc
     1681 ttccaagggc ccgcaggttg tggtctcgcg cgccgtgccg gagctggtat cgcatttgtt
     1741 ccagaccgaa gtgccggaga tttacgacaa cacggtggtc atccgtgcca ttgcccgcga
     1801 agccggcgag cgtaccaaga tcgcggtcat gtcgaaggac aaggatgtcg atccggtggg
     1861 cgcttgtgtc gggatgaaag gtatgcgcgt gcagtccatc atccgcgaat tgcgcggcga
     1921 aaagatcgac attatcgaat accacgagga ttcagtggtc tttgccgaga aggcactaca
     1981 gccggcgaag gtcagccgcg taaccatcct cgaaggtcag gagaagcacc tggaagtgat
     2041 cgtcgacgac agccaactgt cgctggcgat cggcaagaag ggacagaacg tccgcctggc
     2101 cgccaagctg ctgggctgga agatcgacat caagagcgaa gaagagaagc gtcaggaagt
     2161 ggaagatcgg atgaacatag tgttgaacca gccgatcacg ccactggaga gcgttcctga
     2221 tctggagcca ggaatcatcg agaagctggt cgccgccggt ataacgacgg tcgaagccgt
     2281 cgccgacatg actccggagc aactcgaaga agtgccgggt atcggcccca agacggtgga
     2341 aaaaatcagt atcgcagtca ataattactt ttccagtttg gacgcggcgg ctaatgccgg
     2401 cagtgaagaa gtccccctat ccgaaggcga ggtgtccgcc gaagaagtgg ccacggaaga
     2461 cgagcctgtt acggaagatg aagctcccac cgaaggcggt ggcgacgaca gcgccacggg
     2521 acctgattcg ggtgggcagt ctggcgatgt tgcaggcttg gatgaagagc cggaagcagg
     2581 gccggacagc gtggaagaac tggtcgagac cggccaatac tacgaggccg aagtcgtcga
     2641 agggatcgag gatgctcctc tggccgatga agctgaggtg acgacgcacg agcgtccagc
     2701 cggggaaaac gaaaacgttt ccgatgaaga aaaacctggc gagcagaagt agagagcagg
     2761 gcccacggat gctgttgtgg gccggcaaaa tttacggtga ggatgctatg aaggcttaac
     2821 cgggcgggac aatacggagg caatagggcg gaatgcagaa gattcgaatt aacgacctgg
     2881 caagggagct cgaggtaaag agcaaggcga tcctcgacgc tctcatcaag gtcggtgtaa
     2941 ctgaaaagaa aacgcactcc agttccatcg aagtggacga agcggagcgc gttaagaagt
     3001 actttgcagg gcacgcagag ccgggaacca gtcgcgcctc gcgtgcaccg gcagacgaat
     3061 tcaagcccaa gcttgacctg tcgcacatct ccaaacctgg cgatgtgctg aaggcggtta
     3121 cgcaacgggc cgcccagcct tccgcggttg cacgtccggc cacgcctccg acccatgcca
     3181 cagccgccgg gccgcgacaa accgtcgctc cgccttcagc cgtgcggccg gcgcctccgg
     3241 tagcgaaacc tgtgacatcg gtggcgagcg ccactccagc agctcctgcg gaaccgccca
     3301 agcaggcgcc tcgcttcatt acgcctcagt cggtcgcgcg cccacccgcc gccatcgtca
     3361 tcccaccaaa accgccggta gcggcgcctc cggctacgcc gccagcgcat ctgccatccg
     3421 cgactgggac agcgctacct tccgagccag tgatcgagga ccagataacg gcactcgaag
     3481 aaagtgaagt tttgccgtct acgtccgaag cgatcgaatc cgagattccc actgccgtac
     3541 tgcgcgaggt tccgccagct ctattggaag cacaaccgcc agccacggaa aatcgcgcag
     3601 ttaccggccg tccggttgcg cctgggcagt ctgctcaagg tatcggccgt cctggcctgc
     3661 caacgcgtcc cggacaaccg ggaatcgggg ctaccgccgc gcacgcgcca cgccgtttaa
     3721 tcgttcccca gaccggaccg cgtccggtgt atctagctcc acccgtggct ccgccgcgcc
     3781 ccggcgctcc tattgcgccg cgaggcggta tgccggaacg tggcaagcct atcttccagc
     3841 gtccgcgtcc gggagtgccg ccgcaaggac agacgcggcc accggtgcgt ccgggtgagc
     3901 gccgtggtcc gcatccaacg cgttccgggg cgccaggagc ccgtccaggc ttcggagctg
     3961 gcgcaggcgc cgccccgccg cctcctacgg gacagcgtcc cgccggtcgc ccggcacgtc
     4021 caggacagcg ctatgttcct cgaggcgtga aggaaggccc gatgaagggc tttgttccgc
     4081 cgccgcggct ttcgctttcc agcgagccgc tgcccatcac gcggagcatc accattcctg
     4141 aaggcatcag cgtgaaagac ctggcggaga aattgggaat tcgagccaag gaccttatcg
     4201 ctcgcttgct ggcgcgaggc gttttcgcca ccgtcaacca gacgctcgat tccgacctgg
     4261 cgaccgatat ggctcgtcac ttcggcgccg agacggcggt tatcaccttc gaggagcagt
     4321 tggctaagga cacggtgccg gcagaaggcg ctggcgaaga aggtgcagcc ctggg
//
LOCUS       DATMGX010000569         2804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_163738, whole genome shotgun sequence.
ACCESSION   DATMGX010000569 DATMGX010000000
VERSION     DATMGX010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163738"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..727)
                     /locus_tag="VNR65_09255"
     CDS             complement(215..727)
                     /locus_tag="VNR65_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18898.1"
                     /translation="MVLKALFRLALVATAAVVSPLAPQAEEAPTPLKGGYTIAFPEKP
                     QEKEVTPSANAKTTVYSVNQSDAAFLSGYTEYGKNVDLQIELQADIDAFVKSIGAEMT
                     GQKRTDLISASSGRMPRVDFTFESDKIAGRGIAIVTTPRSVIMVSALSVKPNGLQQVD
                     RFVDSFQISE"
     gene            complement(1089..2366)
                     /gene="eno"
                     /locus_tag="VNR65_09260"
     CDS             complement(1089..2366)
                     /gene="eno"
                     /locus_tag="VNR65_09260"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWJ18899.1"
                     /translation="MTAISDITGREILDSRGNPTVEVDVVLEDGSVGRAAVPSGASTG
                     AHEAVEKRDGGARYLGKGVLEAVDAVNGEIFDALQGMDAEDQRHIDNTLIMLDGTENK
                     GRLGANAILGVSLAVAKAASQAADLAIYRYIGGVNARVLPVPMMNIINGGAHADNPID
                     FQEFMISPLGAPTLAEAVRWGSEVFHTLRKILKEGGHNTNVGDEGGFAPNLKSADEAL
                     SYVMRAIEKAGYKPGEDIAITLDPASTEFYKNGRYELKGEGKSLTSEEMVQVYVDLVS
                     RYPIISIEDGMAEDDWDGWKLITDKLGSKINLVGDDLFVTNPKRLAEGIKKGCANAIL
                     VKVNQIGTLSETLDAVDMAHRAGFKSIISHRSGETEDSTIADIAVATNAGQIKTGSLS
                     RSDRIAKYNQLIRIEEELGDVAVYAGRSILRSA"
     gene            2463..>2804
                     /locus_tag="VNR65_09265"
     CDS             2463..>2804
                     /locus_tag="VNR65_09265"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HWJ18900.1"
                     /translation="MSGIPEPMKKCLVLLSGGQDSAVCLAWALTNYDAVETLGFSYGQ
                     RHSVELECRPKLREAISAAFPVWAPRLGPDHVLDLSVLGAVSDTALTSDAEIAFGANG
                     LPNTFVPGRNLL"
BASE COUNT          567 a          801 c          799 g          637 t
ORIGIN      
        1 gttgctgatc gttgcagcgc tcgtagcgat cggcttttca atgatagtca tcgacctgcc
       61 gaataaaaac tatctatgga taggcgctga agttgtggtc gctgttctct ttattatgtc
      121 tggtgtcagc tacctcaagc aaatttttgg aggcaagcgc taaaataagc ggcttgccga
      181 atttgtatag acgcggaccc gcgtgagtgg tgcctcactc agatatctgg aacgagtcca
      241 cgaaccggtc cacttgctgg aggccgttcg gcttcaccga caaggccgaa accatgatga
      301 ccgaacgggg cgttgtcaca atggcgatgc cacgtccggc aatcttgtcg ctttcgaaag
      361 taaaatcgac ccgtggcatt cgcccgctgc tggcggatat aagatccgtc cttttttgcc
      421 cggtcatttc ggcgccgatt gatttgacga aagcgtcgat atcggcctga agctcgatct
      481 gcagatcgac gtttttgccg tattccgtgt atccgctcag aaaagcggcg tcgctttgat
      541 tgaccgagta tacggttgtt tttgcgttcg ccgaaggggt gacctctttc tcctgcggct
      601 tttcaggaaa cgcgatggta tatccaccct tgagcggagt gggggcctcc tcagcttgag
      661 gggcaagcgg gctgacgacc gcagcggtgg cgacaagcgc taacctgaaa agagccttta
      721 ggaccatggc tggacgtatc tccgaaaaac gaggggccgg ttctgccaga gccgatattg
      781 ctaacaaatc tgcatactgc tcgcaattcg tcagaaggcg gggataagct ccgggaagcc
      841 cgacctcaaa cggacagcaa taagtcacgc ccttgccatc accgttcgtg atgtaaagta
      901 tttatatgat tacttagagc ggtctttttt gccaaacaaa cccaatagca ataagttgtt
      961 aacacgcctt actcacggga aatcctgcag acctcgttgc aagccgggca ggatttctcg
     1021 cgggcgtaat aggcctggaa agctccagtt gacgatatga gtcgaacgct cgtctttgcg
     1081 gccttcggtc aagctgagcg caggatgctc cgcccggcat aaaccgcgac atcgcccagt
     1141 tcttcctcga tgcggataag ctggttgtat ttcgcgatac gatcggaccg cgagagcgag
     1201 cccgtcttga tctgaccggc attcgtggca accgcgatgt cggcaatggt cgaatcttcg
     1261 gtctcgcccg agcggtgcga gatgatggac ttgaacccgg cgcgatgggc catatcgacc
     1321 gcgtcgagcg tctcgctcag cgtgccgatc tggttcacct tcacgaggat ggcgtttgcg
     1381 cagcccttct tgatgccttc ggcaagccgc ttcggattgg tgacgaacag atcgtcgccc
     1441 acgaggttga ttttcgagcc gagcttgtcc gtgatcagct tccagccgtc ccaatcgtcc
     1501 tcggccatgc cgtcctcgat ggagatgatc ggataacggc tcacgagatc gacatagacc
     1561 tgaaccatct cctcggacgt gaggctcttg ccctcgccct tcaactcgta gcggccgttc
     1621 ttgtaaaact cggtggaggc aggatcgagc gtgatggcga tatcctcgcc cggcttgtag
     1681 cctgccttct cgatggcgcg catcacataa gacagcgctt catccgccga tttcagattc
     1741 ggcgcgaagc caccttcgtc gccgacattg gtattgtggc cgccctcctt caggatcttg
     1801 cgcagcgtgt ggaagacctc cgagccccag cgcaccgctt ccgcaagcgt tggcgcgccc
     1861 aagggcgaga tcatgaattc ctggaagtcg atcggattgt cggcatgtgc gccgccattg
     1921 atgatgttca tcatgggcac gggcaggacg cgggcattca cgccgccgat atagcgatag
     1981 attgccaaat ccgccgcctg gcttgcagcc ttcgcaacgg ccagcgaaac accgaggatc
     2041 gcattcgcgc caagacggcc cttgttctcc gtcccatcga gcatgatcag cgtgttatcg
     2101 atgtgacgct ggtcttcggc gtccatgccc tgaagagcat cgaagatttc gccgttcacg
     2161 gcatcgaccg cctccagcac gcctttgccg agatagcgcg ccccgccgtc gcgcttttcg
     2221 acggcctcat gggcaccggt cgaagcgcct gacgggactg cggcgcggcc caccgatcca
     2281 tcttcaagca cgacgtcgac ttcgacggtt gggttgccgc ggctatcgag gatttcgcgg
     2341 cctgtaatgt cagagatggc tgtcatggag aactccgcgc aagggtgagg gatggtaacc
     2401 aaagaacgta atgttgtctt aaaggactcg cccagcctct taaagaggtt taatcggtaa
     2461 acatgagtgg cataccagaa cccatgaaga aatgccttgt cttgctgtcc ggcggccagg
     2521 attctgctgt ctgtcttgcg tgggcgttga ccaactatga cgcagtcgaa acgcttggtt
     2581 tctcgtatgg gcagcggcac agcgtcgagc tcgaatgccg cccaaagctc agggaagcca
     2641 tctcagccgc ttttccggtc tgggcgccgc gtctcggccc cgaccacgtg ctcgatcttt
     2701 cggttttagg cgcggtcagc gacacggcgc ttacgagcga tgccgaaatt gcattcggcg
     2761 caaatggcct gccgaatacg ttcgtgcccg gccgcaatct gttg
//
LOCUS       DATMGX010000570         2849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_109492, whole genome shotgun sequence.
ACCESSION   DATMGX010000570 DATMGX010000000
VERSION     DATMGX010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109492"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VNR65_09270"
     CDS             <1..419
                     /locus_tag="VNR65_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18901.1"
                     /translation="LKAPVKEDICYATQNRQDAVKDLIGRCDRLVVVGSKTSSNSNRL
                     REMADKAGIPGYLVDGPEQLRREWFDGVKTVGVTAGASAPEILVKGVVAQLCLWGGES
                     VEELKGREEHVIFALPRTLREVTKRVGSLSSDSHTG"
     gene            444..519
                     /locus_tag="VNR65_09275"
     tRNA            444..519
                     /locus_tag="VNR65_09275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:477..479,aa:Thr,seq:cgt)
     gene            complement(737..1000)
                     /locus_tag="VNR65_09280"
                     /pseudo
     CDS             complement(737..1000)
                     /locus_tag="VNR65_09280"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
     gene            complement(1070..2389)
                     /locus_tag="VNR65_09285"
     CDS             complement(1070..2389)
                     /locus_tag="VNR65_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HWJ18902.1"
                     /translation="MDSKEPSVAPSAVIGPSKPRLWRVLGPGLITGAADDDPSGIATY
                     SQAGAQFGFGLAWTLLFTYPLMVVIQAISARIGRTTGRGIAGNIRVHYANWLLQTVVG
                     LLFVANTFNIGADLGAMAAASRLLLPAIPTWAYLLVFSFICMAGQIFMHHTRYVAVLK
                     WLTLSLFAYFAVLCVVHIPWLELLKGLLWPRFLPDRDFWLTVVAILGTTISPYLFFWQ
                     AAQEVEDTKAEPVREPLRRRPSQGPDALARIQLDTIVGMGISNLVALAILATTAATLH
                     AGGVRDLTSAAQAAEALRPIAGRFAFALFALGIIGTGLLSVPVLAGSAAYALGEARRW
                     PVGLSRQPLQAKAFYATITAATLMGAAANVLSISPVKALVWSAVVNAIVAVPVMALLM
                     RMATNVKILGEFTISRAWTIIGWLATGVMAIASTVFLISLLLPVAQQ"
     gene            complement(2650..>2849)
                     /locus_tag="VNR65_09290"
     CDS             complement(2650..>2849)
                     /locus_tag="VNR65_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18903.1"
                     /translation="LRERARKNLGDGGITSSYKGDPKKWAIAILQSVPATEFNCVLRY
                     TMHAVCRRARWSLRGSPHTPE"
BASE COUNT          636 a          810 c          864 g          539 t
ORIGIN      
        1 tgctcaaggc gccggtgaag gaggacatct gctatgccac ccagaatcgt caggacgcgg
       61 tcaaggatct gatcgggcgt tgcgaccggt tggtggtggt cgggtcaaag acgagttcga
      121 attcgaatcg gctgcgcgag atggcggaca aagccggcat accgggatat ctggtcgatg
      181 ggccggagca actgcgtcgc gagtggttcg acggggtcaa gaccgtgggg gtgacggccg
      241 gcgcctcggc tcccgagatc ttggtcaagg gcgttgtcgc ccagctgtgc ctgtggggag
      301 gcgagtcggt cgaggaactc aaggggcgtg aagaacacgt cattttcgcg ctgccgcgaa
      361 ccctgcgcga agtcacgaag cgggtcggca gcctttcgtc ggatagccac acgggttaga
      421 atgcccgccc ttttggcgtg catgccgcaa tagctcagtt ggtagagcaa cgcattcgta
      481 atgcgtgggt caggggttcg aatcctcttt gcggcaccat aattgccagg gtttcatcaa
      541 tacgaccatt ggcggccagt cgactgtaag tgctgcgtaa gtgcagtatc acgggaagat
      601 aaagatagac gtggcaaccg tagcaaggag cgatgcgcca ccggctttgc gcgataattc
      661 ccccattatc gatgcatacc agccaaccgc aaattccgct ggctcgattt gagtccgata
      721 gttcagcagc ggtggattat cgtcctatcc cgcgcgtcct agaaaaatcc cagctgacgt
      781 tcattcccac cgcgatgccg cgcagctctc gccctaggtg cagctccatc accggccgcc
      841 acggcacgag actgaaccca atgccatcgt cgagcatcgc gaagcgcccg ctcgcgaggt
      901 caatcgaacg gcgatacacg ccgcggaccg actcgccgtc gtgcgtttcc cgataggtca
      961 agccggagtc ggccgagatc gattgactca ccgcggcaag ttcgcggccg tagacctgcg
     1021 aggactacga gctctggatc gaccgggttc aacccaaacc ggcctggttt cattgttgag
     1081 caacgggtag gagcagtgag atcaggaaga cagtcgacgc gatagccatc acaccggtcg
     1141 cgagccagcc aataattgtc cacgctcgag agatcgtaaa ctcgcccagt atcttcacat
     1201 tggtcgccat acgcatgagg agcgccatga ccggaacggc gacgatggcg ttgaccacgg
     1261 cagaccacac caacgctttg actgggctaa tcgacagcac attcgccgcg gctcccatga
     1321 gggttgcggc ggtgatcgtg gcataaaagg ctttagcctg aagtggctgg cgtgacagcc
     1381 caacgggcca gcggcgagcc tcgcccagcg cgtacgcggc cgatcccgcc aagactggca
     1441 ctgacaagag cccagtgccg atgatgccta gggcaaataa ggcgaaagca aagcggccgg
     1501 caatcgggcg cagcgcttcg gctgcttgcg cagcgctcgt caagtcgcga acgcctcctg
     1561 cgtgcaatgt tgcggctgtc gtggcgagaa tcgccaatgc gactaaattg gatataccca
     1621 tgccgacaat ggtatcgagt tgtattcgtg caagcgcatc tgggccctga ctcgggcggc
     1681 ggcgtagcgg ctctcgtact ggctcggcct tggtgtcctc gacctcttgg gcagcctgcc
     1741 agaaaaacaa atacggactg atagtggttc ccaagattgc gacgacggtc agccaaaagt
     1801 ctcgatcggg caaaaatcgc ggccatagca ggcctttgag aagctcgagc caaggtatgt
     1861 gcacgacgca gagcaccgcg aaatacgcaa acagcgagag cgtaagccac ttcaatacgg
     1921 caacgtagcg cgtgtgatgc atgaagattt ggcctgccat gcaaatgaaa ctaaatacca
     1981 acaggtatgc ccatgtcggg atggctggta gtaataggcg acttgcagcg gccattgcgc
     2041 ccaaatcggc accgatgttg aacgtattgg caacgaacaa gagcccaacc acggtttgca
     2101 gcagccagtt ggcgtagtga acccggatat tgccggcgat gccgcggccc gtggttcgcc
     2161 cgatgcgtgc gctgatcgcc tggatgacaa ccattaacgg gtaggtgaag agtagcgtcc
     2221 aagcgagacc aaacccaaac tgggcaccgg cctgcgaata tgtcgcgatg ccgctcgggt
     2281 catcatctgc tgcgccggtt attagcccgg gaccgagtac gcgccacaag cggggtttgc
     2341 tggggcctat cacggcgctc ggcgctacag agggttcctt gctatccaaa caaatgctcc
     2401 caacgcaatt gaagctaaga tcgagcgaca ccaccagttt gcaaagcacc tggcattcga
     2461 aaagcggtcg gattcttctc aaggaagacc cagctacgac tactaagtgc agtgacgctg
     2521 gtctattgct gttgcgggtc catctatgtg agcgtgtggg gcgtgtacag ctacccgcac
     2581 atggaggtta tagcctaacg cactttagct gggaactgct aaaccgccca ccaaccgtgg
     2641 ctcaagagat tattccggcg tatgcggcga tcctcgtaaa ctccatcggg cccggcggca
     2701 caccgcgtgc atggtgtagc gaaggacgca attgaattca gtggcaggca cggattgcag
     2761 gattgcgatc gcccattttt tgggatctcc cttgtacgag gacgtgattc cgccatcacc
     2821 aaggttcttg cgagcgcgct cccgtaagc
//
LOCUS       DATMGX010000571         2769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_32841, whole genome shotgun sequence.
ACCESSION   DATMGX010000571 DATMGX010000000
VERSION     DATMGX010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32841"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(692..994)
                     /locus_tag="VNR65_09295"
     CDS             complement(692..994)
                     /locus_tag="VNR65_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18904.1"
                     /translation="MQDVAVDFLRRVRSIIEAAEQGLVREDNLSAVRTSMNQLLEQDW
                     VMPRAVSDVSIHWRLIEDSLRTFLKGKADVPTSTEELQTAWTACRAIENVFKEDQQ"
     gene            1557..2072
                     /locus_tag="VNR65_09300"
     CDS             1557..2072
                     /locus_tag="VNR65_09300"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HWJ18905.1"
                     /translation="MNNHSAFFQLLDELGIASKTVEHEAVLTAEGLAQRDIGELEFPV
                     KNIVVADRGGQFYLVTMHLLTPPLDLKQLAKSIGADGRFSFASPEILAAALKILPGSV
                     TPFAVFNDVERKVRVILDSRMRTAETVSAHPLVNTLTTTIAMADLLKIFDHSGHRPRW
                     CALPLKQAGSS"
BASE COUNT          622 a          725 c          701 g          721 t
ORIGIN      
        1 tggccaatcc tcgaggggtt gagcctgcga gtgaatcgcc aaagcttaag cttctcgaga
       61 ttgaatgaga cattcgttgc aatttagtca gtgtagctgt gctttttgcg cggctattcc
      121 actgggtttt taattcgcac actcagaact gttgcataat agcattctcg atataagctt
      181 gtatgcaaca ggacagagat ttgaacatgg caggagttgc cagtttctgt ccaagaaggt
      241 tttcaagctc ctaagtcgcc caagccccta gcgttacttc atcgctaggg gctttcttgt
      301 tgtgcttcaa tcgcgtagga aaaaacctca taggttggat aacctttact gaccttgttt
      361 gggctgcggc ttcacctggc cccgtaacca atccgtgata ggcaacggat tgttcataaa
      421 atcctgaaaa gatcgaagat gttgtaagtg aacgagtagc agttaaatgt catctacctt
      481 gtatgatata gagctctggc gcgagtatgc ggagcagatg cgagcgctca gccaaagggt
      541 gaccgatcct cgggtagagc ggcgtgctct tgctgctgct gcaggctttg agcgcattgc
      601 ggacctggcc agcaagttgc gatccagcag cgagagccga cgacgctggg gttggcctga
      661 cggacgtgct gtctatccct aaatgcgagc tctattgctg gtcttctttg aaaacgtttt
      721 caatggcgcg gcaggcagtc caagcggttt gcaactcttc tgtcgacgtc ggtacgtcgg
      781 cctttccctt cagaaaggtt ctgagagagt cctcgatcag tctccaatgg atgctcacat
      841 cggagacggc tcgtggcatc acccaatcct gctcaagcaa ttggttcatg gaggttcgta
      901 cggcggagag attgtcctcc ctgacgaggc cctgctccgc ggcttcaatg attgaacgta
      961 cgcgccgaag gaaatctact gcgacatcct gcatgggaca ccaaatgaag aaagtttcaa
     1021 ctattgcatc gaggcgatga ttcgctccta caccagacta gtggtccgat tccatttgat
     1081 ccgctttgat gcacactcca gagcaaatgt ctcgcaatca taaggatcac cagtatcttt
     1141 tgatcctggt agagcgcttt ttgacatttg agcacgaact cgcaggcggg actcaaatgt
     1201 caaattcgat ccatcccgaa gaatattctc aacgcgcggt tttccgaaat caataaatga
     1261 acatccctaa cggctgggag aggggttttg gggcgccgcc agggaccgag cgccttgtga
     1321 acgcgcctcg cacctgctta tctagatttc atctgccaat ctctctaact ttgccataat
     1381 tcgcgccggt tgcagtgggt atcgtgttgt tcgaacatgc cgcgcgctcg cctgaaaagc
     1441 gcgttcggtc gccactctat agtccatcaa cttgtacgcc ttcgatccgt acagatcaaa
     1501 ggcgggctga tctaggccct cgacctttca gttatcgttc ctcacatact ctcccgatga
     1561 acaaccacag tgcctttttc caactccttg acgaactcgg catcgcttca aaaacggtgg
     1621 aacacgaagc cgtgcttact gccgagggcc tcgcgcaacg agacattggt gagttagagt
     1681 ttcccgtcaa aaacatcgta gtggctgaca ggggcggcca gttttatctc gtgacgatgc
     1741 accttctcac gcctccgctg gacctaaagc aactcgcgaa gagcatcgga gcggacggcc
     1801 gtttttcctt cgcctcgccg gaaatattgg cagccgctct caaaatcctg cccggtagcg
     1861 tcacgccttt cgcagttttc aatgatgttg agaggaaagt ccgcgttatt ctcgacagcc
     1921 gaatgaggac ggcagagacc gtaagcgccc atccgctcgt caacaccttg accacgacta
     1981 tcgccatggc cgacctgctc aaaatcttcg atcactccgg ccatcggccg cgctggtgcg
     2041 ctctaccgct gaagcaggca gggagctctt aacttcgctg tccagatgtg cttgtttgca
     2101 tgcaatctgc ggtgtatctt agatgtgagg cactcctcac agtatacaat gtgtcgattt
     2161 tggggtgtcg ggagggttat tccctcattc ctcgttcatt ttaacgtgat ttcgatagat
     2221 aatctcggat cttaaaaatt agctaatgtc agtttcaacc gaaggatggg aggagcatcc
     2281 gatgacctaa aagccgtaaa catatcgaac ctgcactttc cacatgcctg tttggttttt
     2341 ttagtgatga gtatgtcgtg gaagtttctt ataaggctcg tgttgatcct cttttttgga
     2401 tttctcactg ctgtttctgt attcgctccg gttcatcaaa agcgcgctga tcttcgattg
     2461 ggcgcgtcgg atcgttccct gcaagaattc agcaatttaa gcgcagacga aatcaatcgc
     2521 cgcaccctga aagccgcggc cgcaattaac gccgcccgaa gataaaatct ctaccggcat
     2581 tgcgcgttgc ttggtcctag accgctcgcg tgggctttct ggtgagggag ccggaacttt
     2641 ttatcgctga cacggcgggt tctcttgttt ctgttccaaa gccgcccgtc gcagcctgag
     2701 cggatggctc agagcactat aataagtttt cttttgcgag aagcagcgga cacgtaagaa
     2761 atccgcctt
//
LOCUS       DATMGX010000572         4565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_307528, whole genome shotgun sequence.
ACCESSION   DATMGX010000572 DATMGX010000000
VERSION     DATMGX010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307528"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VNR65_09305"
     CDS             <1..362
                     /locus_tag="VNR65_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ18906.1"
                     /translation="RLRVVENRFVIDVIDNGCGLPKENRHRLIEPYITTRQKGTGLGL
                     AIVQRITEQHGGVLQLADAPTSEGYSHGAWISMSVPVSLSADKADKDEAKPNGQPRST
                     APDQTATHGKEGLSHGV"
     gene            352..1749
                     /locus_tag="VNR65_09310"
     CDS             352..1749
                     /locus_tag="VNR65_09310"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWJ18907.1"
                     /translation="MASDILIVDDEADIRELVSGILEDEGHKTRLAKDSDEAIRAVEE
                     RRPSLVILDIWLQGSKLDGLEVLAKIKKQHPDLPVVIISGHGNIETAVTAIKRGAYDY
                     IEKPFKADRLIVVTMRAIEAFRLRRENKELKERSSYSSDLIGKSPVMNHLRQSIQKVA
                     PTNSRVMITGAQGSGKELAARFLHACSQRENGPFVAVNAASMAADRVEIEFFGVEETG
                     QSTRKVGALEEAHNGTLYIDEVADMPADTQGKLLRVLVEQKFQRIGGGPKVSVDVRIV
                     SSSSRDLEREMSQGRFRQDLFHRLNVVPLRVPSLVERREDIPELVEHFVKQIALSSGL
                     PPRVIGDDAMAVLQSHEWQGNVRQLRNIVERVMIMSNSDPEAVITADMLPDDIGANVP
                     LSSNGSGGEHLMSLPLRDAREIFEREYLMAQINRFGGNISRTAEFVGMERSALHRKLR
                     ALGVNSNERAGDTRH"
     gene            1929..2807
                     /locus_tag="VNR65_09315"
     CDS             1929..2807
                     /locus_tag="VNR65_09315"
                     /inference="COORDINATES: protein motif:HMM:NF005209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HWJ18908.1"
                     /translation="MTRIVYVNGAYTPYADAAIHVEDRGFQFADGVYEVCEVRDGSLV
                     DERRHMRRLGRSLLELGMTEPMGLRSLGVILRETIRRNRVKNGLVYLQVTRGVASRDF
                     AFPGPAVAPSIVCFARSLSRSKAEKKAAAGISVVTVPDIRWKRVDIKTTGLLAQALAR
                     QQAKEAGADEAWMVDGQGFVTEGASCNAWIVTGAGNLVTRSAESGILRGITREVAMEA
                     ARGKGLNLEERLFTVEEAKSAREAFQTSASGLVMPVVKIDGHVIGSGRPGEIALELRK
                     IYHNVAESSPPHELLA"
     gene            2953..3201
                     /gene="hfq"
                     /locus_tag="VNR65_09320"
     CDS             2953..3201
                     /gene="hfq"
                     /locus_tag="VNR65_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWJ18909.1"
                     /translation="MAPDKQQNLQDAFLNHVRKNKTPLTIFLVNGVKLQGIVTWFDNF
                     CVLLRRDGHSQLVYKHAISTIMPGGPISFLDGDQNGDR"
     gene            3207..>4565
                     /gene="hflX"
                     /locus_tag="VNR65_09325"
     CDS             3207..>4565
                     /gene="hflX"
                     /locus_tag="VNR65_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWJ18910.1"
                     /translation="MKKLAPREISEYRAGEQENTAPIDGLTRAVVIVPVRKFPHFQGS
                     QASESARLTSSERLSEATGLSKAIGLDVCFSGTVPLTAPRPATLLGSGKVEELAAIIK
                     AEDVTVAIVDHMLSPIQQRNLEKAWNAKVLDRTGLILEIFADRARSKEGRLQVELARL
                     TYQKSRLVRSWTHLERQRGGFGFLGGPGETQIEADRRAIGERIDTIRSELAVVERTRR
                     LHRKRRKATEQQVVSLVGYTNAGKSTLFNAFSGAGVLAKDMLFATLDPTLRRIEFPEG
                     GAFLLSDTVGFISDLPTQLIAAFRATLEEIIEADLILHVRNIAAESSDAERIEVEKVL
                     TEIGIGPEEQGRRVIEVWNKADLLPGEERASLSKRAAQAEPAALLTSAATGEGVTELA
                     ALIRARLQKDMRTRNVIVDDGNGALIHWLYENTEVLRRCDLDGGGLQFEVRLAHDRQR
                     DLD"
BASE COUNT          985 a         1290 c         1411 g          879 t
ORIGIN      
        1 tacggcttcg cgtggtcgaa aaccggttcg tcattgacgt gatcgacaat ggctgcggat
       61 tgccgaaaga gaaccgccac cggctgatcg agccctacat tacgacccgg caaaagggta
      121 cagggcttgg tttggccatc gttcagagga taaccgagca gcacggtggc gtgctgcaac
      181 ttgccgacgc tcctacgagc gaaggctatt cacatggcgc ctggatcagc atgtccgtcc
      241 cggtgtcgct cagtgccgac aaggcagaca aagacgaagc aaagccgaat ggccagccgc
      301 gaagcacggc gcccgaccag acggcaacgc atgggaagga gggactaagc catggcgtct
      361 gacattctga tcgtcgacga cgaagccgat attcgcgaac tcgtgtcggg gatcctggaa
      421 gatgaaggac acaagacgag gctcgccaaa gatagcgacg aggcgatccg cgccgtcgag
      481 gaaaggcggc cgtctctcgt catcctcgat atctggctgc aaggctcgaa gctggacggg
      541 ctggaagttc tggctaagat caagaaacag catcccgacc ttcccgtggt catcatcagc
      601 ggtcatggca acatcgagac tgcggttacc gcgatcaagc gcggagccta cgattacatc
      661 gaaaagccct tcaaggctga caggctgata gtcgtcacca tgcgcgcgat cgaggccttc
      721 aggctgaggc gcgaaaataa agagctgaag gaaaggagtt cttacagctc cgatctgatc
      781 ggcaaatcac ccgtcatgaa ccatctgcgc caaagcatcc aaaaagtggc gcccacaaac
      841 agccgcgtga tgatcacggg ggcgcaaggg tccggcaagg agcttgccgc ccgcttcctt
      901 cacgcttgct cgcagcgcga gaatgggccc ttcgtcgccg tcaatgcagc gtcaatggct
      961 gcggaccggg tcgaaatcga gttcttcggg gtcgaggaga cggggcagag cacgcgcaag
     1021 gtgggcgcgc tcgaggaggc gcacaacggc accctctaca tcgatgaggt cgccgacatg
     1081 cccgcagata cccagggcaa gctcctgcgc gtgctggtcg aacagaagtt ccagcgcatc
     1141 ggcggcggcc cgaaagtctc ggtcgacgtg cgcatcgtct catcgtcaag ccgcgatctt
     1201 gagcgcgaga tgagccaggg ccgcttccgt caggacctct ttcatcgtct gaacgtggtg
     1261 ccgctgaggg tgccttcact ggtcgagcga cgcgaggaca ttcccgaact cgttgaacat
     1321 tttgtgaaac agatcgcgct ctcctccggg cttccgcccc gggtaatcgg cgacgatgcc
     1381 atggccgtcc tccagtcgca tgagtggcag ggcaacgtgc gtcagctgcg caatatcgtc
     1441 gagcgcgtga tgatcatgtc caacagcgac ccggaggctg tgatcacggc cgatatgctg
     1501 cccgacgata tcggagcgaa tgtccctttg tcgtcgaacg gctcgggggg tgagcacctc
     1561 atgtcgctgc cactccgcga tgcgcgtgaa attttcgagc gtgagtatct catggcgcag
     1621 atcaaccgtt ttggcggaaa catatcacgc actgccgaat ttgtgggcat ggagcgctcg
     1681 gctctccacc ggaagctccg ggcgcttggc gtgaactcga acgaacgggc cggggacacc
     1741 aggcactaga gcagcccgcg tccgtttcga tgcggataag ctggtcaagc acttttagaa
     1801 tgatcaactt ttccgcctta atcgattccg attaaaggcg ggttgatcgg gcgcccttgc
     1861 gcttcataag ccggatgcat tttggcaata taattgcagg ttgaaggctt gcgcaggaag
     1921 ggcggaccat gacacggatt gtgtatgtaa acggcgcgta taccccttac gccgatgccg
     1981 ctatccatgt cgaagaccgc ggttttcaat ttgccgacgg cgtttacgaa gtgtgcgaag
     2041 ttcgcgacgg ttctctcgtg gacgagcggc ggcacatgcg ccggctcggg cggtcgcttc
     2101 ttgaactcgg gatgacggag ccgatgggct tgaggtcgct tggcgtgatc ttgcgcgaaa
     2161 ccatccggcg caaccgcgtc aagaacggac ttgtctatct ccaggtgacg cgtggcgtag
     2221 cgagccgcga cttcgctttt cctggacccg ccgtagctcc aagcatcgtc tgctttgcgc
     2281 gttccctgtc gcgcagcaag gctgagaaaa aggctgccgc cgggatctcc gtcgtcaccg
     2341 tccccgacat ccgttggaag cgcgtcgaca tcaagacgac agggcttctc gcgcaagctc
     2401 ttgcacgcca gcaggcgaag gaggccggcg ctgacgaggc ctggatggtc gacggccaag
     2461 gcttcgtcac cgaaggcgca tcctgcaacg cctggatcgt gacgggcgct ggcaatctgg
     2521 tgacgcgctc ggcagagtct ggtatcctgc gaggaataac gcgcgaggtt gcgatggaag
     2581 cagcccgcgg gaagggtctt aatcttgagg aaaggctctt cacggtcgag gaggcgaaat
     2641 cagcgaggga ggcttttcaa acgtccgctt ccgggcttgt gatgccggtc gtcaagatcg
     2701 acggccatgt catcggcagc ggaagacctg gcgaaattgc tctggaatta cgaaagattt
     2761 atcacaatgt ggcggagtcc tctccgcctc acgaactttt agcatgaccg aaagggactg
     2821 atggactatc agcggcaagc accgcttagg cgtggccagt atggttgtgc caagaaccga
     2881 gacagtagca ggtgggataa tcgggtagtg ggcaagcggc caagagtgcc gcgctgaaaa
     2941 aacggatagg acatggctcc agacaaacaa caaaatttac aagacgcctt tctcaatcat
     3001 gttcggaaaa acaagactcc tcttacgatc tttctcgtca acggtgttaa gctgcagggg
     3061 atcgtgactt ggttcgacaa tttttgcgtt ctgctcaggc gcgatgggca ttcacagctt
     3121 gtctacaagc atgccatctc gacaatcatg ccgggcggcc ctatttcgtt tcttgatgga
     3181 gatcaaaacg gagaccgtta ggacagttga agaaactcgc accccgcgaa atttctgaat
     3241 atcgcgccgg agaacaagag aacacagcgc caatagacgg gctaacgcgc gctgttgtca
     3301 tcgttccagt ccggaagttt ccgcactttc agggatctca agcttctgag tccgctcgtc
     3361 tgaccagttc ggagcgtctc agcgaagcga ctggcctctc aaaagcaatt ggtctcgacg
     3421 tttgcttcag cggcactgtt ccgctcacag ccccccggcc agcaacgctt ctcggttctg
     3481 ggaaagttga ggaactcgct gcgatcatca aggctgagga tgttacggtt gcgattgtcg
     3541 accacatgct gagcccgatc cagcagcgca atctcgagaa ggcgtggaac gctaaggttc
     3601 tcgatcggac ggggctcatt ctggaaatct tcgccgaccg ggcgcgcagc aaggaaggca
     3661 ggctgcaagt cgaactggct cggctcacct atcagaaaag ccgcctcgtc agatcctgga
     3721 cgcaccttga gcgccagcgg ggcggtttcg gctttctggg cggcccaggc gagacgcaga
     3781 tcgaagccga ccggcgcgcc atcggcgagc gtatcgatac gatccgcagc gaacttgccg
     3841 tggtcgagcg tacgaggcgg ctgcaccgca aacgccggaa ggcgaccgag cagcaggtgg
     3901 tttcccttgt tggctacacc aacgccggca agtcgacgct cttcaacgca ttctcgggcg
     3961 ctggcgtgct tgcaaaggac atgctgtttg cgacgctcga cccgacgctg cgccggattg
     4021 aatttcccga aggcggcgcg ttcctgttgt ccgatacggt cggatttatt tccgaccttc
     4081 cgacccagct tattgcagcc ttccgggcca cgttggaaga aatcattgag gccgatctga
     4141 tcctgcatgt tcgaaacatc gccgccgaat cctccgacgc cgagcgcatc gaagtggaaa
     4201 aggtgctgac cgagatcggg atcgggcccg aagagcaggg cagacgggtc atcgaggtgt
     4261 ggaacaaggc tgatcttttg ccgggggagg agagggccag tctttcaaag agggcggcgc
     4321 aggccgagcc tgccgctctg ttgacgtcgg ctgcaaccgg cgagggcgtg acggaactgg
     4381 cagcactgat ccgcgcccgg cttcagaagg acatgcgaac ccgcaacgtg attgtcgacg
     4441 acggcaacgg cgcgctcatt cattggctgt atgagaatac ggaagttctc cgccggtgtg
     4501 atctggacgg tggaggcttg cagttcgagg tgcgcctcgc acacgataga cagcgcgatc
     4561 ttgac
//
LOCUS       DATMGX010000573         3611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_195086, whole genome shotgun sequence.
ACCESSION   DATMGX010000573 DATMGX010000000
VERSION     DATMGX010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195086"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            434..2959
                     /locus_tag="VNR65_09330"
     CDS             434..2959
                     /locus_tag="VNR65_09330"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HWJ18911.1"
                     /translation="MHTWLAVLRRLSSIRSSAKLLTAALTLFLLNAGTAFAQPNEAAG
                     EANLRLPDLSSVRFLGLTGHNLLLIGLLFCAFGLLFGMAIYMQLKNLPVHRSMREISE
                     LIYETCKTYVITQGKFLMVLWAFIAVVIVAYFGWLAPVPGKPVSVTLPVILLFSIVGI
                     CGSYGVAWFGIRVNTFANSRTAFAGLRGKPYPIYAIPLKAGMSIGMMLISVELLIMLC
                     ILLFVPADYAGQCFIGFAIGESLGAAALRIAGGIFTKIADIGSDLMKIVFKIKEDDAR
                     NPGVIADCTGDNAGDSVGPSADGFETYGVTGVALITFILLGVTPAVFIRNGMAADAAA
                     AMASRVQVQLLVWIFVMRVMMLVSSTVAYFLNEAVAKARFGDANEMNYEAPLTSLVWL
                     TSIISIVLTYIVSYLIVPVLGGDSTQWWKLATIISCGTLAGAIIPELVKVFTSTNSGH
                     VKEVVTSAEEGGASLDILSGLVAGNFSCYYLGFTMVLLMSIGYYVSTYGLGFLMLAPA
                     VFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSMIEHIPNIQGELKRDYNIDV
                     NFERAKHMLEENDGAGNTFKATAKPVLIGTAVVGATTMTFSIIMALTRGLTENVGKLS
                     LLHAPFVLGLISGGAIIYWFTGASMQAVTTGAYRAVEFIKANIKLDSAEKASVSDSKK
                     VVQICTQYAQKGMFNIFLAVFFVTLSFAFVEPFFFIGYLIAIAISGLYQAMFMANAGG
                     AWDNAKKIVEVELKQKGTALHDATVVGDTVGDPFKDTSSVAMNPIIKFTTLFGLLAVE
                     LAVSLTASKGAAFSHVLAVIFFAISFVFVYRSFYGMRIVKAAA"
     gene            3180..>3611
                     /locus_tag="VNR65_09335"
     CDS             3180..>3611
                     /locus_tag="VNR65_09335"
                     /inference="COORDINATES: protein motif:HMM:NF026155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18912.1"
                     /translation="MRELQRQIESLYAMGTDAAVFPGASEIFSQFRNALTAGTIRAAE
                     KIDGRWRTNEWVKQGILLGFRIGKLAESGDPRVLSFVDKDTYPIRHFTASDLVRVVPG
                     GSSVRAGAYVAPSVICMPPMFINVGAYVDEGAMIDSHALVGS"
BASE COUNT          660 a         1084 c         1062 g          805 t
ORIGIN      
        1 cgcaggaacc cagtttcccc taagacgttt gcgcgcaatt gattggaccg cgaatcgccg
       61 acccaccctc gctccgtgac cgaagtgtca ttgaccccgc tttgcccaac atgctataaa
      121 gactcgcctt ttgtactatg cctgtggtgt gcctcaagat gcacacgaat aggtgcaagt
      181 gttcccgacg cggccaaacc tcaacacttc tcgtcttgaa gtggaagcgc gtttccggga
      241 ttccagcagg tccatgcatg gttttttctg gcgatctgcg aagacgcgag ctacagcctt
      301 gtacgcctga tctgttgggc ggtaatcgat ctcatggaaa cgctgtctgc gatggatcgc
      361 ggaagagcgg aacaaaccta acgtcatttt atgagtgcgc cccattcacg ggtgcgtcca
      421 tcggaggaat catatgcaca cctggctcgc cgtcctgcgg cggctgtcat ccataagaag
      481 ctcggccaag ttgctcaccg ccgcgctgac tctgttcctg ctgaatgccg ggacggcttt
      541 cgctcagccg aacgaagccg ccggcgaggc caaccttcgg ctgcccgatc tttcgtcggt
      601 cagattcctc gggttgacgg gccataacct gctgctgatc ggcctgctct tctgcgcctt
      661 cggcctgctg ttcgggatgg cgatctacat gcagctcaag aacctgccgg tccaccgctc
      721 catgcgcgag atctccgagc tgatctacga gacctgtaag acgtacgtga tcacccaggg
      781 caagttcctg atggtgctgt gggcgttcat cgccgtcgtt atcgtggctt acttcggatg
      841 gctggcgccg gtcccaggca aaccagtgtc agtgacgctg ccggtcattc tgctgttcag
      901 cattgtcggc atctgcggca gctacggtgt ggcgtggttt ggcatccggg taaacacctt
      961 cgccaactcg cgcaccgcct tcgccggact gcgcggcaag ccctatccga tctatgccat
     1021 cccactgaag gcaggcatga gcatcggcat gatgctgatc tcggtcgagc tgctcatcat
     1081 gctttgcatc ctgctgttcg ttccggctga ttacgccggg cagtgcttca tcggcttcgc
     1141 catcggcgaa tcgctgggtg cggcggccct gcgtatagcc ggcggtatct tcaccaagat
     1201 tgccgacatc ggctccgacc tgatgaagat cgtcttcaag atcaaggaag acgacgctcg
     1261 caacccgggc gtcatcgccg actgcaccgg cgacaacgcg ggcgactcgg ttggccccag
     1321 cgccgacgga ttcgagactt atggcgtgac cggcgtcgcg ctgatcacgt tcattctgtt
     1381 gggcgtcact cccgccgttt tcattcgtaa cggaatggct gctgacgcgg cggctgccat
     1441 ggcctccagg gtgcaagtcc aactgctggt gtggatcttc gtcatgcgtg tcatgatgct
     1501 ggtctccagc acggttgcgt atttcctgaa cgaagccgtc gccaaggcgc gcttcggcga
     1561 tgccaacgag atgaactatg aggctcctct cacctcgctg gtgtggctca cgtccatcat
     1621 ctccattgtg ctgacctaca tcgtgtcgta cctgattgtg ccggtcctgg gtggcgattc
     1681 gacgcaatgg tggaagctgg ccacaatcat ctcctgcgga accctggcgg gagcgattat
     1741 cccagaactc gtgaaggtgt tcacctccac caattcgggg cacgtgaagg aagtggtgac
     1801 ctcggccgaa gaaggcggcg cctcgctcga cattctgtcg ggcctcgtgg ccggcaactt
     1861 ctcctgttac tacctgggct tcaccatggt cctgctcatg agtatcggct actacgtgag
     1921 cacctacggg ctgggcttcc tgatgctggc gccagccgtg ttcgctttcg gactggtagc
     1981 cttcggcttc ctgggcatgg gaccggtgac catcgcggtg gattcctatg gcccggtcac
     2041 cgacaatgcg caatcggtgt acgagctttc tatgattgag cacataccga atatccaggg
     2101 tgagttgaag cgcgactata acattgacgt gaacttcgag cgcgccaagc atatgttgga
     2161 agaaaacgat ggcgccggca acaccttcaa agccaccgcc aaaccggtgc tgattgggac
     2221 cgccgtcgtg ggcgcgacca ccatgacctt ctccatcatc atggcgctca cccgcgggct
     2281 gacggaaaac gtgggcaagc tctcgctgct gcacgctccc ttcgtgctcg gcctgattag
     2341 cgggggcgcc attatctact ggttcaccgg cgcttccatg caggcggtaa ccaccggcgc
     2401 ttaccgcgcg gtggagttca tcaaggccaa catcaagctg gacagcgcgg agaaggcctc
     2461 ggtgagcgac agcaagaagg tggtgcaaat ctgcacccag tatgcacaga aaggcatgtt
     2521 caacatcttc ctcgcggtgt tcttcgtgac cctgtccttc gccttcgtgg agccgttctt
     2581 cttcatcggc tacctgatcg cgattgccat ctccggcttg taccaggcga tgttcatggc
     2641 caatgccgga ggcgcgtggg acaacgccaa gaagatcgtc gaagtcgaac tgaaacagaa
     2701 gggcacggcg ctgcacgatg caaccgtcgt cggcgacacg gttggcgatc ccttcaaaga
     2761 cacgtcctca gtcgctatga acccgatcat caagttcacc acgctgttcg ggttgctggc
     2821 ggtagagctg gcggttagtt tgaccgcgag caagggagcg gcctttagcc acgtgctggc
     2881 ggtcatcttc ttcgcgatat cgttcgtgtt cgtgtaccgg tcgttctacg ggatgcggat
     2941 cgtcaaggcc gcagcctgat tttcaaccac gccaacggcg aacgccatcc cttacgggat
     3001 ggcgttttgc attgaagggg cgcaccttca ggtgcgccgc ataagttcct tcaattttgt
     3061 catcccgagc ggagcgaagc ggagtcgagg gatctgcggt tattcgccga ggtccagcgc
     3121 tgcccgctcg cgctgatgcg ggctggagcg ggcggctgct aacagttata ctgcccgcca
     3181 tgcgcgaact tcagcgtcaa atcgagtctc tctatgcgat gggaactgat gcggctgtgt
     3241 ttcccggcgc cagcgagatc ttttcgcagt tccggaatgc actgactgcg ggcacgattc
     3301 gtgctgccga aaagatcgac ggccgttggc gcaccaatga atgggtgaaa caagggatcc
     3361 tgctgggctt ccgtatcggc aagctggcgg agagtggaga cccgcgtgta ctctcgttcg
     3421 ttgacaagga cacctatccg attcgccact tcaccgccag cgacctggtg cgcgtggttc
     3481 ccggaggctc gtccgtgcgc gctggcgcct atgtagcccc ctccgtcatt tgcatgccgc
     3541 cgatgttcat caacgtcggc gcgtatgtgg atgagggtgc gatgatcgac tcgcacgcgc
     3601 tggtcggttc c
//
LOCUS       DATMGX010000574         2787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_291241, whole genome shotgun sequence.
ACCESSION   DATMGX010000574 DATMGX010000000
VERSION     DATMGX010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291241"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="VNR65_09340"
     CDS             complement(<1..1692)
                     /locus_tag="VNR65_09340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWJ18913.1"
                     /translation="MTGIDGVSPLFAEALAKQGYTTLTEVQRAVLAPDVGEADLLVSA
                     QTGSGKTVAFGLAMAPTLLRGKDHFPENSDPLAVVVAPTRELAVQVRLELEWVYAPAG
                     ARMAACVGGMDMRQERRALANGAHIVVGTPGRLRDHITRGSLDMSSVRALVLDEADEM
                     LDLGFREDLEFILKAAPPERRTLLFSATVPKPIETLAKRFQKNAIRISANTGTRQHAD
                     IDYRAIAVASRERENAVINVLRYFDAGSALVFCATREGVKHLASRLGNRGFGVVALSG
                     ELSQVERTRALQAMRDGRAQVCVATDVASRGIDLPNLDLVVHADLPRDSEALLHRSGR
                     TGRAGRAGVSVIIAQHSKRRSIERLLTIAGIKATWMQVPSAEIILQRDRERLLSDPAL
                     EGPLNDEELAFAKELLATKSPEQIAAAFFRQNKTQRPTPEEFVDTGEDASEVATARSD
                     FEGGVWFKLSVGRKQRAEPRWILPLICKAGGVTKRDIGAIRIFEAESMVEVSARSAQS
                     FSRALASSGRREDSITITPMNGGPEQRAGLADTPRSAAMKGNRKKPGERRKKTPQS"
     gene            1973..>2787
                     /locus_tag="VNR65_09345"
     CDS             1973..>2787
                     /locus_tag="VNR65_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HWJ18914.1"
                     /translation="MALPLKIAFLGSEGVPFIKSGGLADVVGALPKFLSARGHDVIVI
                     LPKYASIDASRWTLEPFLSPLGVWMGHTEEWCAAFTATFAGIRFFFIESNQYFERKGI
                     YHDPEMEDYGDNARRFGFFTRAALQLCHDMGFAPDIVHAHDWQTALAPAYLKIWHWDD
                     AVLGGAASVLTIHNIGHQGKYPASDYDYLGLQRDNFTPAKFEDFGGINMLKGGIVYAD
                     LVTTVSPTYAVETRTDIGGAGLAPVLNDKGDNYLGILNGVDGDAWNPATDALIP"
BASE COUNT          525 a          874 c          816 g          572 t
ORIGIN      
        1 agattgcggc gtcttctttc gccgctctcc tggctttttc cggttccctt tcattgctgc
       61 cgaccgaggc gtatccgcga ggcccgcgcg ctgctcaggc ccaccgttca tcggcgtaat
      121 cgtgatcgaa tcctctcgcc gccccgaact ggcgagagca cgactgaagc tttgagcgga
      181 gcgcgcgctg acctcaacca tgctttcggc ttcgaagatc ctgatggcgc cgatgtcgcg
      241 cttggtgacc ccgccagcct tgcaaatcag tggcagtatc cagcgcggct ccgctctctg
      301 cttccgcccg accgagagct tgaaccagac accaccctcg aaatcgctcc gcgcagtcgc
      361 aacttcgctg gcgtcttctc ctgtatccac gaactcttcc ggcgtcgggc gctgggtctt
      421 gttttggcga aagaacgccg ccgctatttg ctccgggctt ttcgtggcga gcaattcctt
      481 ggcgaaggca agctcttcgt cgttaagcgg gccttcgagc gcggggtcgc tcaggagccg
      541 ttcccggtcg cgctggagga taatctctgc gctcggaact tgcatccagg tcgcctttat
      601 ccccgcaatg gtcagaagcc tttcgatgct ccggcgcttg ctgtgctgcg cgatgatgac
      661 gctgacgccg gcgcggcccg cgcgccccgt tcgtccgctt cgatgcaaga gcgcttcgct
      721 gtccctcggc aaatcggcgt gaaccacaag atcgagattt ggcaaatcga tgccgcgcga
      781 ggccacgtcg gttgcaacgc aaacctgcgc tcggccatcc cgcatcgcct gaagcgcccg
      841 cgtccgttcg acctggctca gttcacccga cagggcgacg accccgaacc cgcgattgcc
      901 aagacggctc gcgagatgtt tcacgccttc ccgcgtggcg cagaagacaa gcgcgctgcc
      961 cgcgtcaaaa tagcgaagaa cattgatgac ggcattttcc cgttcgcgcg acgcaacggc
     1021 aatcgcgcga taatcgatat cggcgtgctg ccttgtgccc gtattggcgg aaatccggat
     1081 ggcattcttc tgaaagcgct tggcgagcgt ttcgatcggc ttcggcaccg ttgccgagaa
     1141 gagcagggtg cggcgctccg gcggagccgc cttgagaatg aactcgaggt cttcacgaaa
     1201 gcccaggtcg agcatttcgt cggcctcgtc gagaaccaag gcgcgcaccg acgacatgtc
     1261 gagcgacccc cgcgtaatgt gatcgcgaag acgtccgggc gtgccaacga cgatatgcgc
     1321 gccattggcg agcgcgcggc gttcctgccg catgtccatg cccccgacgc aggctgccat
     1381 ccttgcgcct gccggtgcat agacccattc gagttcgagg cgcacctgga ctgccagctc
     1441 gcgcgtcggc gcaacaacga cagctaaggg gtcggagttt tctgggaagt gatctttccc
     1501 tctcagcaag gtcggggcca tggcgagacc gaaggcgacc gtcttgcctg atcctgtctg
     1561 cgcggagacg agaagatcgg cctcacccac atccggcgca aggacggcgc gctgcacttc
     1621 ggtgagcgtc gtatatccct gtttcgcaag cgcctccgcg aataagggag acaccccatc
     1681 aatacccgtc atgtgaagcg ctttctctca tttttggagg cggtgaagcg atgtgacgtc
     1741 aacccttagc gcaaaagagg ctcagagaga taaagatttc caaaaggtgc ttgattgtgc
     1801 agctcattga cccggaaagc cccgctaatc caccgtcatt cgccggtttt cgagcctgtt
     1861 gggcaaaact ggcctgcctt gggcgttgcg gcaacgggag cttgagaatc aggtagacga
     1921 caaagactaa ccggcagatg atccgacgac gtgcatccca gcaggatccc gcatggccct
     1981 ccctctcaaa attgctttcc tcggatccga aggggtcccg ttcatcaagt caggtgggct
     2041 cgccgatgtc gtcggtgccc tgcccaagtt tctcagcgct cgtggtcacg acgtcatcgt
     2101 catcctgccg aaatacgcgt cgatcgatgc cagccgctgg acgctcgaac cgttcttaag
     2161 tccgcttggg gtctggatgg gccataccga agaatggtgt gcggccttca ctgcgacgtt
     2221 tgctggaata cgatttttct tcatagaatc aaatcagtat ttcgagcgca agggcatcta
     2281 ccatgatcct gagatggagg attatgggga taatgcgcgg cgctttggct tctttacccg
     2341 tgcggcgcta caactttgcc acgacatggg cttcgcaccc gatattgtgc atgcccacga
     2401 ctggcagacc gctcttgctc cggcctacct caagatctgg cattgggacg acgcggtact
     2461 aggcggcgcg gcgagcgtct tgaccatcca caacatcggt catcagggca agtacccagc
     2521 gtcggattac gactacctcg ggctgcagcg cgacaacttc acgccggcca agttcgagga
     2581 ctttggcggc atcaacatgc tgaagggcgg catcgtctat gccgatctgg tgacgacggt
     2641 gagtcccacc tatgcggtcg agacgcgcac agacatcggg ggcgccgggc tcgctccggt
     2701 cctcaacgac aagggtgaca actatttagg catcctcaac ggggttgatg gcgatgcatg
     2761 gaacccagct acggacgccc tgatccc
//
LOCUS       DATMGX010000575         2431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_219663, whole genome shotgun sequence.
ACCESSION   DATMGX010000575 DATMGX010000000
VERSION     DATMGX010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219663"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..656
                     /locus_tag="VNR65_09350"
     CDS             420..656
                     /locus_tag="VNR65_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HWJ18915.1"
                     /translation="MGSARRKKNVRLKGTVKWFNNAKGFGFIGREDGSADVFVHYSAI
                     NIEGYKSLQEGDQVEFEIVDGQKGPQAGNVTRVQ"
     gene            831..>2431
                     /gene="polX"
                     /locus_tag="VNR65_09355"
     CDS             831..>2431
                     /gene="polX"
                     /locus_tag="VNR65_09355"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HWJ18916.1"
                     /translation="MDNKAIANVFYETADLMEINGDDSFRIRSYRRAAEALEGLPQQV
                     SGLIGEPKKLLEIPGIGKGMAANIQELCRSGKLGPHQELLVKYRPSMLELLKIQGLGP
                     KTIALLWSAFQVSDLGGVEKLAREGKLRELPRLSEKSEQEILKAIEDYRRISGRFLLD
                     EADRTAQKLTEHLRHIKGIEKITPAGSLRRGRETVGDLDVLITGPCCVDDEQRAGLIE
                     EIVRFPGIVEVLAKGDNKVSFKLRSGMQVDVRTLPPESYGAAMQYFTGSKNHNVSLRQ
                     RALKMGYTLNEYGLFRLDNNKRVAGATEDEIYGKLKLDCIPPELRENFGEIEAAEKHN
                     LPDLITVADLRGEVHMHTVETDGKCTIDEMAAAAKERGYKYIAITDHSKNLAFANGLD
                     DKRAEQHIARIHAANEQTDGITIMAGIEVDILADGELDLSDSVLEQMDVVVASVHSAF
                     NQEPQQMTDRLLRALNNKNVSILGHPTGRLLLRRDAYPFDMDSVLKAALKNRVAMELN
                     AYPDRLDLNDVHLRMARERGVKIVI"
BASE COUNT          580 a          673 c          698 g          480 t
ORIGIN      
        1 gacttcggtg ttattaaccg caatctatat atacctattt atgatcggct taataacacc
       61 gaagtccctc agtggctaaa gccaccatca atgacagcag cttacggcac ggctgaagcc
      121 gtgcccgttc aaagcgtgag atgctcatga ataatccaga tgaagcgccg ctctccccgt
      181 accctcgccc ggacttcact aattcgaaaa ctgctcaaaa acccgcttca aatggggctt
      241 tggtctatat gctctcttga cgacaccacc aaacggtagg aaacttaagg tacgctgttc
      301 aacaggccaa tccaactggc cagatgtcat ctgggaaaaa agtcctccat cgactttggc
      361 ttctgggctc aacctgattg acgcaattca agaccagcat cctcggggtg gggatgctga
      421 tgggctctgc caggaggaag aagaacgtga gactgaaagg aaccgtaaag tggttcaata
      481 acgcaaaagg ctttggcttc ataggccgtg aggatggtag tgcggacgtg ttcgtgcact
      541 acagcgcgat caacatcgaa ggctacaaga gcttgcagga aggcgaccaa gtcgaattcg
      601 agatcgtcga tggtcagaaa ggccctcaag caggcaacgt gacgagggtc cagtaaggtc
      661 cttcgcagtg tttccctgaa gcccagccgg cgaagctggg tttttcatta gggggtcaat
      721 tggttcgtcg cctttctgct ccccgtgttc ggtggatctt agattctgag caagactgaa
      781 tcgcaccgca tgtcccttct ccacgttaca attcctctcg ggtgtcttgc gtggacaaca
      841 aggccatcgc caacgtcttt tacgaaaccg ccgatctcat ggagatcaat ggcgacgact
      901 ctttccgcat ccgctcgtat cgccgcgccg ccgaagctct cgagggactg cctcagcaag
      961 tctcggggct gatcggcgag cccaagaagt tgctggagat tcccggcatc ggcaaaggca
     1021 tggctgcaaa catccaggag ttatgccgga gcggcaaact tggtccgcac caggagttgc
     1081 tagtaaagta ccggccctcg atgctggagt tgctgaagat ccagggactg gggccgaaaa
     1141 cgatcgctct gctttggagc gccttccaag taagcgatct cggtggagtg gagaagttgg
     1201 cgcgcgaagg caagctccgc gaactgccgc gtctgagcga aaagagcgag caggaaatcc
     1261 tgaaggcgat tgaagattac cgccgcattt ccgggcgctt cctgctcgac gaagccgacc
     1321 gcaccgcgca gaagttgacc gagcacctgc ggcacatcaa agggatcgag aagatcacgc
     1381 ccgccggttc gctgcgccgt ggacgggaga cagtcggcga tctcgacgtg ctgattaccg
     1441 gaccgtgctg cgttgacgat gagcagcgcg ctggtttgat cgaagaaata gtgcgctttc
     1501 ccggtatcgt cgaagtgctg gccaagggcg acaacaaagt cagcttcaaa ctgcgtagcg
     1561 gaatgcaggt ggacgtgcgc accttgccgc cggaatccta tggcgcggcc atgcagtact
     1621 tcaccggctc caagaaccac aacgtctcgc tgcgccagcg tgcgctcaag atgggttaca
     1681 ccttgaacga atacggacta tttcgcctgg acaacaataa gcgtgtggcc ggtgcgactg
     1741 aagacgagat ttacggcaag ctcaagctcg attgcatccc gcccgaactg cgcgaaaact
     1801 tcggagagat cgaagccgcc gaaaagcata atctcccgga cttgatcacc gtcgccgatc
     1861 tgcgcggtga ggtccacatg cacacggtcg aaaccgacgg gaagtgcacc atcgacgaga
     1921 tggcggcggc ggcgaaagag cgcggctaca agtacatcgc catcaccgat cactcgaaaa
     1981 atctggcttt cgccaacggc ctggacgaca agcgtgccga gcaacacatc gcccgaattc
     2041 atgccgccaa cgagcagacg gatggcatca ccatcatggc aggcatcgag gtggatatcc
     2101 tggccgatgg cgagctcgat ctctccgact cggtgctgga gcagatggat gttgtggtcg
     2161 ccagcgtaca ttcggcattt aaccaggagc cgcagcagat gaccgaccgt ttgttgcggg
     2221 cgttgaacaa caagaacgtc tcgattctcg gacaccccac cggacgcctg cttctacggc
     2281 gtgacgccta tccgtttgac atggattcgg tcctgaaggc tgcgttgaag aacagagtcg
     2341 cgatggagct gaacgcgtat ccagatcggc tcgatttgaa cgacgtgcat ctgcgcatgg
     2401 cgcgggaacg cggcgtgaag atcgtgatca a
//
LOCUS       DATMGX010000576         3713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_113090, whole genome shotgun sequence.
ACCESSION   DATMGX010000576 DATMGX010000000
VERSION     DATMGX010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113090"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /locus_tag="VNR65_09360"
     CDS             complement(<1..1798)
                     /locus_tag="VNR65_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWJ18917.1"
                     /translation="MRTGICVGLVILLTLALAPAYAQAPSAALSAKSADAAAIAARAS
                     LGPLRIIVHHQASSGPGHRTLHTPSENLPAILSENRAAQESIVTAHFGPVAALTGRER
                     ALRLMGITPAFAINATAAEIEALANNPDVLNIAIDKVERPILIQSVPLIGMTNAYSNY
                     NATGTGFAVAIIDSGVNKTHTFLANVILEACYSTTTGTLGSGGSASTCPGGVSSSTAS
                     GSGVNCNIAWSGCEHGTHVAGIAAGNNTQFQSGQPPNGVAKASGIIAIKAASEFTGSD
                     CSPDPSPCIAFWDSDMQAALDHVYAVRNSLPAGYQGVASANLSIGDGSFSSNCNRTNV
                     SFKFSIDNLLSANIATVIAAGNDGSTSQISFPACISSAIAVSASSKNDVIAAYTNINS
                     QVAVFAPGGDFSQPIFGAANDIILSSVPAGLTNCNSIGYSFPGPNPTTGGSYCYLAGT
                     SMATPHVAGAFAAIRSALPNATVAQILSALKSTGTPITDTRVGGTITKPRIRVDLALQ
                     SLANSFGTLQVSPTTNISSSGQQGGPFSPSSFNYTIAASIGTRNYAISGIPNWLTASS
                     TSGTASTSGTTVTFNINSNANSLSPATYNATIT"
     gene            complement(2027..2518)
                     /locus_tag="VNR65_09365"
     CDS             complement(2027..2518)
                     /locus_tag="VNR65_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18918.1"
                     /translation="MKGIGSLHFSARMIGVPCRAFIVAIACFASLSVAHAEVQVSGSR
                     DAVTVQAKNALLGDIIVAINSTLNVKISVAPAINVPITGTYSGTLRRVFARMLAGHDF
                     ILNSSGDRMTIIVSTQGGPGPVQGAPAKIDTARARANVAATDDEANTSGVQGWCGAFT
                     FPK"
     gene            complement(2722..>3713)
                     /locus_tag="VNR65_09370"
     CDS             complement(2722..>3713)
                     /locus_tag="VNR65_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18919.1"
                     /translation="PTPAYVSAGDGVDAGACPVTAPCATLNYALSQITSGGQVIIVKA
                     GVFGPIVLDTKVSISGAEPNVDTQIVGDTTAAVVGCVGSAPAATCNGHGNAGYAVDIV
                     AGVNDKVQIGHVLMSAGPNGNGALRLLSGGQLQIGNDVFRGNGTVTNAIVSLVPNNPG
                     TTQAQVYFSNSDVGFNNSANSSSGAVLVQPIGTTSLKLHFNHVEVHNTSFGIRTDSTS
                     LSAGASVATFISDSEFFSFPNAAVNAFSLGAVAPVNAAFDTTRILNAGAALRANGSLS
                     FVVLTNNTVSGNAIGVQVTNGATVLTSVNNTITGNGPTNNQNVVGTITNAPLQ"
BASE COUNT          797 a         1038 c         1097 g          781 t
ORIGIN      
        1 acgtaatcgt tgcattgtaa gtcgcgggtg aaagactgtt cgcgttcgaa ttgatgttaa
       61 aagtgaccgt ggtgccggag gtggaggcgg tgcccgatgt cgacgaggcc gtcagccaat
      121 tcggaatgcc ggaaatcgcg tagttcctgg tcccgatcga agccgcgatc gtgtagttaa
      181 aggacgacgg cgagaacggc ccaccctgct gaccggaaga gctgatattg gtcgttggcg
      241 atacttgcaa tgtgccaaag ctgtttgcca gcgactgcag ggcaagatcg acgcgaatgc
      301 gtggcttggt aatggtcccg ccaacgcggg tatccgtgat cggcgttccg gtcgatttta
      361 atgcgctcaa gatctgcgcg accgtcgcgt tcggcagggc agacctgatt gccgcgaaag
      421 cgccggcaac gtgcggcgtc gccatcgaag ttccggccag gtagcagtaa ctgcctccgg
      481 tcgtcgggtt tggtcctgga aaggaataac cgattgaatt gcaattcgtg agtccggccg
      541 gcactgacga cagaattatg tcgttcgccg cgccaaagat cggttgggaa aaatccccgc
      601 ctggcgcgaa taccgcgacc tgcgagttga tattggtata cgccgcaatc acatcgttct
      661 tgctcgaagc cgatacggcg atggccgagg aaatgcaggc tggaaagcta atttgactgg
      721 tactgccatc gttaccggcg gcaataaccg tcgcgatgtt ggctgacaaa agattgtcga
      781 tcgaaaactt aaacgacacg ttcgtgcggt tgcagttact cgaaaaactg ccgtcgccga
      841 tgctcagatt ggccgaagcg acgccctgat accctgcagg caggctgttg cgaacggcgt
      901 agacatggtc cagcgcggct tgcatatcgg agtcccaaaa ggcgatacac ggagacgggt
      961 ctgggctgca atccgaaccg gtaaattcgg atgccgcttt gatcgcgatg atgccggagg
     1021 ctttcgcaac gccattgggc ggttgacccg actgaaattg ggtgttgttt ccggccgcga
     1081 tgccagcgac gtgggtgccg tgctcacagc cactccaagc gatattgcaa ttgacgcccg
     1141 agccggacgc ggtcgatgac gataccccgc cgggacaggt ggaagccgag cctcctgagc
     1201 ccaacgtccc ggtggtcgtc gaatagcagg cctccaaaat cacattggcg aggaatgtat
     1261 gagtcttgtt gacgcccgag tcgatgatgg caaccgcgaa accagtaccg gttgcgttgt
     1321 aattgctgta ggcattggtc atcccgatca gcggcacgga ctgaatcaga atcggccgct
     1381 ccaccttatc gatagcgata ttcaaaacgt ccggattatt cgcgagcgcc tcgatctccg
     1441 ccgccgtggc gttgatcgca aacgccggcg tgatccccat gagccgaagc gctcgctcac
     1501 gaccggtcaa agccgcaacc gggccgaagt gagctgtaac gatcgattcc tgggctgccc
     1561 ggttctcgct caagatcgcc ggaaggtttt cactgggcgt gtggagggtt cgatgtcccg
     1621 ggccacttga agcctgatga tgaacaataa tgcgcaacgg accgagtgac gcccttgcag
     1681 caatcgcagc ggcatccgcg cttttggcgc tcagtgcagc gcttggcgcc tgcgcataag
     1741 ccggcgccaa ggcgagcgtc aaaaggatca ccaagccgac gcagattccc gtacgcatag
     1801 tggcagcaaa ccgcattttg gtgacccggc agaccacgag cttttcgcgc tgatcggcta
     1861 acgaattcgc gctatttgcg ggtgattcgt gcgcaagtat agattatacg ggcccacagc
     1921 ctatgtcgtt ttccggtttt tgttttgacc ggggctagct gcaaatagtt agcatgcgct
     1981 tatcgagatc cggcgacttc aacaaaaagt gagtcgggac agatttttac tttgggaacg
     2041 taaatgcgcc acaccagcct tggaccccgg aggtgtttgc ctcgtcatcg gtagccgcga
     2101 cgttagcgcg cgcgcgcgct gtatcgattt tcgccggcgc cccttgaacc gggcctgggc
     2161 ccccctgcgt cgacacaata atcgtcatcc ggtcacccga ggaattcagg ataaaatcgt
     2221 gccccgccag catccgcgca aagactcgcc ggagagttcc tgaataagta cccgtgattg
     2281 ggacattgat cgccggcgca acactgatct tgacattcag cgtggagtta atcgcaacaa
     2341 taatgtcacc gagcaacgcg ttcttggctt gcaccgtcac tgcgtctctt gaaccggaaa
     2401 cctggacctc ggcatgggca acgctcaatg atgcgaaaca agcaatcgcc acgatgaagg
     2461 cccggcaggg gacgccgatc atccgcgcgc tgaaatgcaa tgacccgatg cccttcatct
     2521 tattccgccc gcctttcgta ctcgcccgtc gcggccacta atttaccggc ccatcacgag
     2581 atgccgacgc tatcctcttg agactattgg cgcttgcgcc ccctatcgac ggcatcaagc
     2641 ccacattaaa gaaaaactcc gggcagtgaa accgcccgga gttgcgcttt cagatgacgt
     2701 ctttggcgac ggccataagc gtcactgtag tggtgcgttc gttatcgtgc cgaccacgtt
     2761 ttggttgttg gtggggccat tgccggtgat cgtattgtta accgatgtga ggacggttgc
     2821 gccattggtt acctgcacgc cgattgcatt gcccgaaacc gtgttgttgg tcaggacgac
     2881 gaaggatagc gagccgttcg ctctgagcgc ggccccagca ttgagaatgc gagtggtatc
     2941 gaacgcggca ttgaccggtg cgactgcccc aaggctgaat gcattcaccg cggcgttggg
     3001 gaacgagaag aactcggagt cggaaatgaa ggtcgcgaca ctcgcgcctg ccgagagcga
     3061 cgtgctgtcg gtgcggatgc cgaaactggt attgtgcact tcgacgtgat tgaagtgcag
     3121 cttcaacgag gtggttccga tcggttgcac gaggacggcg ccgctggaac tgttggcgct
     3181 attattgaag ccgacgtcgc tgttggagaa atacacctgc gcttgcgttg tgccggggtt
     3241 atttggaacg agcgagacta tggcattggt caccgtgccg ttgccgcgga aaacgtcatt
     3301 gcctatctgt agctgcccgc ctgataacaa tctcagcgcg ccatttccat taggaccggc
     3361 actcataagg acatgaccga tctgaacttt atcgtttaca cccgcgacga tatcaacggc
     3421 gtagccagcg ttgccgtggc cattgcaagt agctgcaggt gctgagccaa cgcagcccac
     3481 taccgccgcc gtggtatccc cgacgatttg agtgtccacg ttcggttcag cgccactgat
     3541 ggaaaccttg gtatcaagaa cgattggtcc aaaaaccccg gctttcacaa tgatgacctg
     3601 accgccgctg gtaatttgac tcaaggcata attcagcgtg gcgcatggcg ccgtgaccgg
     3661 acatgcgccc gcgtcgacgc catcccctgc ggaaacatag gccggcgtag gaa
//
LOCUS       DATMGX010000577         2294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_123897, whole genome shotgun sequence.
ACCESSION   DATMGX010000577 DATMGX010000000
VERSION     DATMGX010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123897"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VNR65_09375"
     CDS             complement(<1..369)
                     /locus_tag="VNR65_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18920.1"
                     /translation="MKLRFVTRTVHAYLDYPVAISLMALPFVLGLGVENPVAKWLSVA
                     TGVAAFVLTFLTNHETGVIKVVPYWVHVAVDRLVGLVFIAAPFAFGFGGVDALYYWAF
                     GATVLIVTVALSAPSQENLRV"
     gene            complement(465..1016)
                     /locus_tag="VNR65_09380"
     CDS             complement(465..1016)
                     /locus_tag="VNR65_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HWJ18921.1"
                     /translation="MSRIHTIPSIDAAPTAAKPLLEGAKKQLGTVPNLFRVIANSPAA
                     LEGYLNLNGALSKGSLDAKTRERIAIAVAEINGCAYCLSAHVYLGRTLAKLDDTEITA
                     NRNGASNDPKADAAVSFAVKVVGQRGHVSDTDVAAVKAAGFDDAQVIEIVLHVALNTL
                     TNYVNEVAKTEIDFPVVAQKKAA"
     gene            1138..2040
                     /locus_tag="VNR65_09385"
     CDS             1138..2040
                     /locus_tag="VNR65_09385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ18922.1"
                     /translation="MDRFESMSAFVAVVEAGGFSAASRKLGMPLATISRKVSELEEQL
                     RAQLLVRSTRKVALTEAGTQYFETCRRHLEDLAEAERQASGEYSAPKGGLIVSAPVVF
                     GRLYLAPIVIAFLKAYPDVDVELRLSDAIVNLIEEHTDIALRIGQLPDSGLMAVRAGL
                     IRHFVCASPRYLAERGSPQHPRDLCAHDCITFTVLHSPTEWTFARGKTIDRIPVRSRL
                     AVSTAEAAADAAVAGVGITRLLCYQVSKAITEGSLTLLLRDFEPAPLPVHLVHSSGRL
                     IPKKLRAFLDFVLPRLKASLVFEP"
     gene            complement(2103..>2294)
                     /locus_tag="VNR65_09390"
     CDS             complement(2103..>2294)
                     /locus_tag="VNR65_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18923.1"
                     /translation="LPAWLPDVTLTDLRLGQERFEIRFWRDGNDTNFEVLKGRKDAVM
                     RMSAALTWNMAGSEGTPLG"
BASE COUNT          431 a          677 c          702 g          484 t
ORIGIN      
        1 aaccctaagg ttttcctggc ttggcgcgct cagcgccacg gtcacgatca gtactgtcgc
       61 gccgaaggcc cagtagtaca gggcatccac cccgccaaaa ccaaacgcga acggcgccgc
      121 gatgaacacc aatccgacca ggcggtcgac ggcgacatgg acccaataag gcaccacttt
      181 gatgacgccc gtctcgtggt tcgtcaaaaa ggtgaggacg aaagcggcga ctcccgttgc
      241 gacagagagc cattttgcga ccgggttctc cacgccaagt ccgagaacga agggtaacgc
      301 cataaggctg atagcaaccg gataatcgag ataggcgtga acggttcttg ttacaaaacg
      361 gagtttcatg gtctttctcc agagtgtgag gttgaggttg ggttcccctc cgagggggat
      421 gcggggcgca cagccaaccg atggctgccc ccgccgaccc acagtcacgc tgcctttttc
      481 tgcgcaacca cagggaagtc gatctcggtc ttggcaactt cgttgacgta gttcgtgagc
      541 gtgttgagcg cgacgtgaag gacaatctcg atcacctgcg catcgtcgaa gccggcggcc
      601 ttcacggcag ccacgtcggt gtcgctgaca tgcccgcgtt gaccgacaac tttgacggcg
      661 aagcttacgg ccgcgtcagc cttcgggtcg tttgatgcgc cgttccggtt ggcggttatc
      721 tccgtgtcat cgagcttggc gagggttctg ccgaggtaaa catgcgccga gaggcagtag
      781 gcgcagccgt tgatttcggc gacggcaatc gcgatgcgct cgcgggtttt ggcatcgagg
      841 gagcctttcg acaaggcgcc attgagattg agataacctt ccagggccgc cgggctgttg
      901 gcgatgacgc ggaagagatt agggactgtg ccaagctgct tcttggctcc ttcgagcaag
      961 ggcttagcgg ccgtcggggc ggcgtcgatg gaggggatgg tgtggatgcg gctcatcttt
     1021 aggttccttc cgaggccggg tgcttgagtt cggccgaccc ccaggatgta accccttcct
     1081 aactgcgaga gtatctgtca ttactgggag atactgttcc aatttatggg aggatgcatg
     1141 gaccggttcg agtcgatgtc cgccttcgtg gccgtggtcg aggccggggg attctcggcc
     1201 gcttcccgaa agctcggcat gccgctcgcc accatcagcc gcaaggtgtc ggagctcgag
     1261 gagcaactgc gtgcgcaact cctcgtgagg tccacgcgaa aggtcgcgct cacggaagct
     1321 ggaacgcagt atttcgaaac ctgccggcgg cacctcgagg atctcgcgga ggcggaacgt
     1381 caggcgtctg gcgagtacag tgcgcccaag ggcggcctta tcgtcagcgc tcccgtcgtt
     1441 tttgggcgcc tctatctggc accgatcgtg atcgcgttcc tgaaagccta tccggacgtg
     1501 gatgtcgaat tgcggctctc agatgcgatc gtcaatctca tcgaggagca caccgacatt
     1561 gctttaagaa tcggccagct gcctgacagc ggccttatgg ccgtgagagc ggggctgatc
     1621 cgccattttg tctgtgcaag tccgcgttat ctagccgagc gcggcagccc gcagcacccg
     1681 cgcgacttgt gcgctcatga ctgcatcacc ttcacggtgt tgcattcgcc gactgagtgg
     1741 acgttcgcgc gcggcaagac aatcgaccgc attcctgtgc gttcgcgttt ggccgtgagc
     1801 accgccgaag ctgcggccga cgcggcagtt gcgggtgtcg gcatcacccg cttgttatgc
     1861 tatcaggtct caaaagcgat cacggaagga agcttgacac tgctgttgag ggatttcgag
     1921 cctgcgccct tgccggtcca ccttgtccat tccagcgggc ggttgatccc caaaaagctg
     1981 cgtgcttttc tcgattttgt cttaccaagg ctgaaggcaa gccttgtgtt cgagccgtag
     2041 ccgctgtgct ctgcaagctt gctcaaaccg gcgatgataa gccaagccgc tgtctttgat
     2101 gtctaaccga gcggggtacc ttcactgcct gccatgttcc acgtaagcgc ggcactcatc
     2161 cgcataaccg cgtccttcct gcctttcagc acctcaaagt tcgtatcgtt gccgtcccgc
     2221 cagaaacgga tttcgaagcg ctcttgcccc agccgcaggt cggtcagcgt cacatccggc
     2281 agccaagcag gcag
//
LOCUS       DATMGX010000578         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_19562, whole genome shotgun sequence.
ACCESSION   DATMGX010000578 DATMGX010000000
VERSION     DATMGX010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19562"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VNR65_09395"
     CDS             <1..306
                     /locus_tag="VNR65_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18924.1"
                     /translation="KPKAGAPSQGCCEPYRTMYAEFWYGARKVVAPVRRGPAGDEVLL
                     PGEIWVPCQLSCEYTLRKQTLHYLESQGAGSGSVFAPVYPRADSYVDGWGQRHGYLF"
     gene            complement(303..1079)
                     /locus_tag="VNR65_09400"
     CDS             complement(303..1079)
                     /locus_tag="VNR65_09400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWJ18925.1"
                     /translation="MVSEFLGIIAALTAIIAARYLLLSGVFYWLLWQRGGEKLHAKRL
                     NRDRPMSAIVMQEIRLSLLSSVVYAVPAAIVLVALLHGGTAVYYDWRAYGGVPYLFLS
                     LFIYLVVQDAYYYWAHRLMHDPRLFRWMHAGHHRSRQPTPFASFAFDPAEAALTGWLL
                     PAMVFIIPIHIVLLIALLMFMSVVAVFNHSGWEILPRCLVRGLVGGQLISATHHSYHH
                     IRFDKNYGLYFRFWDRIMGTDTMPQETSPGSRLSMRPGQG"
     gene            complement(1151..1546)
                     /locus_tag="VNR65_09405"
     CDS             complement(1151..1546)
                     /locus_tag="VNR65_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWJ18926.1"
                     /translation="MIAIDSSALVAILRNEPDALLFAEAIGAANSAIVSAVNCFETSM
                     VLAGAAHGDTVWLPLDALLSRAKIAVIPFDTEQAGFARAAFLKYGKGRHPAALNFGDC
                     AAYALAKSRDIPLLYKGADFTKTDIVSAL"
     gene            complement(1543..1884)
                     /locus_tag="VNR65_09410"
     CDS             complement(1543..1884)
                     /locus_tag="VNR65_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ18927.1"
                     /translation="MATPEQIKAARALLRMEQEELARRAGVSVTTIRRLEAADREYAV
                     AEETAAGVQNALHEAGVEFIHDGVRRIRKEADRDLVTDLMKIAEESARLQEGGPKWTE
                     DDLYDETGLPA"
     gene            complement(1917..>2444)
                     /locus_tag="VNR65_09415"
     CDS             complement(1917..>2444)
                     /locus_tag="VNR65_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HWJ18928.1"
                     /translation="LDLIEKDVFRFCWIVDFPFFERDPETQAICFSHNPFSMPQGGLD
                     ALNTQDPLTIRAFQYDIVCNGVELSSGAIRNHKPELMYKAFEIAGYGPEVVDAKFGGM
                     IRALKFGAPPHGGIAPGIDRIVMLLCGADNLRDVTMFPLNQQAEDLLMGAPSEVSGKQ
                     LRELHILLNLPKKDA"
BASE COUNT          514 a          735 c          728 g          467 t
ORIGIN      
        1 aaaccgaagg cgggtgcgcc cagccagggc tgctgcgagc cttatcgcac tatgtatgcc
       61 gagttctggt acggtgcccg gaaggtcgtg gctcctgtaa ggcgtggccc ggcgggtgat
      121 gaggtgctgc ttccaggcga aatttgggta ccctgtcagc tttcctgcga atatacgctt
      181 cgcaaacaga cactccatta tctcgaaagt cagggggcag gcagcggcag tgtgttcgcg
      241 cccgtctatc cgagagccga ttcctatgtc gacggctggg gccaacgcca cggctatttg
      301 ttctagccct gccctgggcg catactgagc cgcgagcccg gtgacgtttc ctgcggcatc
      361 gtatccgtgc ccatgatcct gtcccagaag cggaaataga gcccgtaatt cttgtcgaag
      421 cggatgtgat ggtagctgtg gtgggtcgct gaaatgagct gcccgccgac gaggccgcga
      481 accagacagc gcggcaggat ctcccagccg gaatggttga agactgcgac gacgctcatg
      541 aacatgagaa gcgcgatcag cagcacgatg tgaatcggga tgatgaaaac catggccggc
      601 agcagccagc ccgtcagcgc cgcttcggcc gggtcgaatg cgaatgatgc aaagggcgtt
      661 ggctggcgcg aacggtgatg cccggcgtgc atccatcgga aaagccgcgg gtcgtgcatt
      721 agccggtgcg cccagtaata gtaagcatcc tgtacaacga ggtagatgaa caagctcagg
      781 aaaagatacg ggacgccgcc ataggcgcgc cagtcgtagt agactgctgt gccgccatgc
      841 aagagggcaa cgagaacgat tgcggctggc accgcgtaga caaccgagga gagaagcgag
      901 agccgtatct cttgcattac gatggcgctc atcgggcggt cccggttcaa tcgcttcgca
      961 tggagtttct cgccgccgcg ctgccacaac agccaataga atacgcccga taggagcagg
     1021 taacgcgccg cgatgatcgc cgtcagcgcc gctattatgc cgagaaactc agaaaccatc
     1081 tgcgccgccc cctgccctgt cagccccatc cctgcttggc cagtcaatca tgtcgtgaac
     1141 ccacaacgca ttacaaagcg ctcacaatat cggtcttggt gaagtccgcg cctttataga
     1201 gaaggggaat gtcgcgcgac tttgcgagcg cgtaagccgc gcaatcgccg aaattgagcg
     1261 ctgccggatg ccgtcccttc ccatatttga ggaacgcggc gcgggcgaac cccgcctgct
     1321 cagtgtcgaa gggaatgacc gcgatctttg cgcggctcaa gagtgcatcg aggggaagcc
     1381 acaccgtatc gccatgggcg gcgcccgcca gaaccatcga tgtttcgaag caattgactg
     1441 cagacacaat cgcagagttc gcagcaccta ttgcttcagc aaaaaggaga gcgtcgggct
     1501 cgttcctcaa aatcgcaacc agcgccgagc tatcaattgc aatcacgcgg gcaagccggt
     1561 ttcatcgtag aggtcgtctt ccgtccactt cggaccgccc tcctgaagcc gggcactttc
     1621 ctctgcgatc ttcatcaaat ccgtcaccag gtcccgatca gcctccttgc gtatgcggcg
     1681 cacgccatcg tggatgaatt ccacacctgc ctcgtgcaac gcgttctgaa cgcccgctgc
     1741 tgtctcctct gcgacggcat actcgcgatc cgcagcttcc aggcggcgga ttgtcgtgac
     1801 ggatacgcct gcgcgccgtg ccagctcctc ctgctccatc cggagaagcg cgcgcgcagc
     1861 cttgatttgc tcgggtgtag ccatgagcga aagctatcat ttatggctgt tttccgtcaa
     1921 gcatccttct tcggcaggtt caataggata tggagctcgc gcaactgctt ccccgaaacc
     1981 tcggacggtg cgcccatcag cagatcttcg gcctgctggt ttagcgggaa catcgtcacg
     2041 tcgcgcaaat tatcggcgcc gcagagcagc atgacgatcc ggtcgatacc gggcgcgatg
     2101 ccgccatgcg gcggcgcgcc gaatttcaag gcgcggatca tgccgccgaa cttggcgtca
     2161 acgacttccg gtccatagcc cgcgatctcg aaggccttgt acatgagctc cggcttgtga
     2221 tttcggatcg cgcccgagga cagctccacg ccgttgcaga cgatgtcgta ttgaaaggcg
     2281 cggatggtca gcggatcctg cgtattcaaa gcatccagac cgccctgcgg catcgaaaag
     2341 ggattgtgcg agaagcagat ggcctgggtc tcggggtcgc gctcgaagaa cggaaagtcc
     2401 acgatccagc agaaacggaa gacatccttc tcgatcaggt ccag
//
LOCUS       DATMGX010000579         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_145362, whole genome shotgun sequence.
ACCESSION   DATMGX010000579 DATMGX010000000
VERSION     DATMGX010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145362"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..1407
                     /locus_tag="VNR65_09420"
     CDS             355..1407
                     /locus_tag="VNR65_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWJ18929.1"
                     /translation="MELGIYTFGDITPDPNTGRAIRIPQRYAEILAAAKLADDAGLDV
                     FGVGEHHRLDIPISSPAAILAAIAGGTKRIRLSSAVTILSTLDPVRVFQDFATVDLLS
                     GGRAEVIAGRGAYIESFALFGDDIADYDALFAEKLDLLLKLNDSERVTWEGHFRPPLS
                     NSEISPRPVGKLPIWLGVGGNPESALRAGELGLPMILANISQPPANFVSQIAAYRQRH
                     SERGHDASTLKVAIATHVHVANDSQTALDEFYPHYSAYFREHAPRANLVGEISRDVYE
                     KRAGATGPIFVGSPQQIIDKLMYDRELFAHDRFLGQVDIGGLPYTKVAKSIELLATQV
                     LPAIRRPSSAGVNQVY"
     gene            1636..>2178
                     /locus_tag="VNR65_09425"
     CDS             1636..>2178
                     /locus_tag="VNR65_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ18930.1"
                     /translation="MTKRTFCTLLMSAGLVGGILAGSAASLLAADKAIKIGGLFPMSG
                     PGSYFGAQDKQGVELALEQLNKAGINGYKFEVQYEDSSCSPLPATQAAKRLLDQFKPD
                     VVIGEECSDATLAVMPVMEQAKVPLLNAGSSAIKITDPGNPWTFRIMPNEVMQGVDIA
                     TKAYNQFNARTAVLLHENTNA"
BASE COUNT          491 a          652 c          593 g          442 t
ORIGIN      
        1 tggtcatgca gcccagccaa gatcggaatg gcgacaaaac agcgccagat cgttggactg
       61 ctcaatgcaa gggcgcatcc ttctgtaatg ccaagtgcgt gcgcgcctga ttgtaataag
      121 actgtgtgta ccgaaaacct aaatccggac gtagtggtga tgaggtccgc ccacgaccgc
      181 gtgtgaattt atagatccaa tccgctacaa gaaacgcgct caattgctgg cgctccggga
      241 tcattcgttg ttggaagtca tttgaatgtc cggatcgggt caatcgctac cgaaatgcga
      301 cgtcggctat accgccaaac ttaaaaacga ataccagtta ctgcaggaag tactatggaa
      361 ctaggcatct atactttcgg cgacatcacc cccgatccga acaccggccg cgcgatcaga
      421 attccacagc gttacgccga gatcctcgcc gccgcgaagc tcgccgacga tgccggtctt
      481 gatgtgttcg gcgtcggcga gcaccaccgc ttggacatcc cgatctcatc accagcggcg
      541 atcctggccg cgattgcggg cggaacgaaa cggatccgtc ttagcagcgc cgttacaatc
      601 ctctcgaccc tcgacccagt ccgcgtcttt caggacttcg cgaccgtgga cctcctttct
      661 ggtggccgcg ccgaggtgat cgccggccgc ggtgcttata tcgaatcctt cgcgctcttc
      721 ggcgatgaca ttgcggacta cgacgccctt ttcgccgaaa agctcgatct tctcctcaag
      781 ctaaacgaca gcgagcgcgt cacttgggag ggtcacttcc gcccaccgct gagcaactct
      841 gagatttcac cgcggccggt gggtaagctg ccaatctggt taggcgtcgg cggcaatcca
      901 gaaagcgcgc tgcgggcggg cgaactcggc ctgcccatga ttcttgcgaa catctcccag
      961 ccaccggcca acttcgtcag ccaaatcgct gcctatcggc agcgtcactc tgaaagagga
     1021 cacgacgcaa gcacgctgaa ggtcgccata gcgacgcatg tccatgtggc gaatgactcg
     1081 cagactgccc tcgatgagtt ctaccctcat tacagcgcct atttccgcga acatgcgccg
     1141 cgcgctaacc tcgttggcga gatttctcgt gacgtctacg agaagcgcgc cggcgctacg
     1201 ggtccgatct tcgtcggtag cccccagcag atcatcgaca agctcatgta cgatcgcgag
     1261 ctcttcgctc acgatcggtt tctcggccag gtcgacatcg gcggcctgcc atacacaaag
     1321 gtggcaaagt caatcgaact gctcgcgacg caagtgctgc cggcgatacg caggccctca
     1381 tcagctggcg tcaatcaagt ttattgacga gactgacctg accaggcttt tttttgagca
     1441 agcgttgtaa gaaaatgagc ttggaaagga gcttggccct gccgaagaag aggttcagcg
     1501 cggagcagat cgtgatgctg cttcgccagt cgtattaggt ttgcgacagg gacagcatcc
     1561 aaaaaacggt caggctactc ctattgcagc cgctcgaata aatagctcta atattaagtc
     1621 agatggagga cagccatgac taagcggaca ttttgcacac tactgatgtc ggccgggctc
     1681 gttggcggca ttctcgccgg atcagcggct tcacttttgg ccgccgacaa ggccatcaag
     1741 atcggcggcc tgtttccaat gtcgggcccg ggatcctatt tcggcgcaca ggacaagcaa
     1801 ggcgttgagc tcgcacttga gcagctcaac aaggcgggta taaatggata caaattcgag
     1861 gttcagtacg aagattcgtc ctgcagcccg ctgccggcaa cgcaagctgc caaacgcctg
     1921 ctcgatcagt tcaagcccga tgtcgtgatc ggtgaagaat gttcggacgc aacgcttgcc
     1981 gtcatgccgg tcatggaaca ggccaaggtt ccgctgctca atgccggctc ctccgcgatc
     2041 aagatcaccg atccaggcaa tccctggacg ttccgcatca tgccgaacga agtgatgcag
     2101 ggcgtcgata tcgcgacaaa agcctacaac cagttcaacg cacgcacggc ggtgctgctt
     2161 cacgagaaca ccaatgcc
//
LOCUS       DATMGX010000580         3227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_277227, whole genome shotgun sequence.
ACCESSION   DATMGX010000580 DATMGX010000000
VERSION     DATMGX010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277227"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VNR65_09430"
     CDS             <1..309
                     /locus_tag="VNR65_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HWJ18931.1"
                     /translation="EANVPYDEVFELEDINSEFAQADVAFVIGANDVTNPAAEDDKTS
                     PIYGMPVLQVWKAGTVMFIKRSLASGYAGIDNPLFYRDNTMMLLGDAKKMTEQIVKAL
                     "
     gene            492..659
                     /locus_tag="VNR65_09435"
     CDS             492..659
                     /locus_tag="VNR65_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18932.1"
                     /translation="MIWLIGVALAVVVGLSVFHVYEVPVAIDQLRRFGDDWSAKTLFV
                     ALALVALSKLF"
     gene            800..2719
                     /locus_tag="VNR65_09440"
     CDS             800..2719
                     /locus_tag="VNR65_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWJ18933.1"
                     /translation="MDGSILIQLVVFLAVAAFAAPVAKTLRLGSILGYLLAGAFIGPY
                     GAGKLLGYSPAYADTLRHIAELGVAMFLFLIGLELRLKRLWKMRDAVFGAGIVQLIGT
                     AALLVILIWGINPSSDLSFSQALLVGLALALSSTALVLQTLEEKHELTSRHGRLAFSV
                     LLFQDLAAIPLIAVVPLLAASAPAGSAITFSGVAKALAVIGLIIVVGHYVLDRLYHVL
                     ASTQVREAMIAASLLTVAGVSLLMTEIGLSASLGAFLAGVLLADSSFRHKLQADIEPF
                     EMLLLALFFTSIGMSLNFNALISQPGLIAAAVAMLVSAKILVLYLLGRWQRLSVPSAR
                     RFAIVTSQGGEFAFVVFASAEQGGVLTPGSASALGIIVTLSMVTTPLLLALDDLIRKR
                     IRKAEPVYDKLPSQDGHVVISGFGRVGQVVARILTAKGIPFTALDADPDQVQLVSQFG
                     NKSYFGDASQLSILEAAETGKATAFVLAIDDVEKALKTAELVKTYFPHVPIYARARNR
                     RHVHQLMDLGVKHIERETFVSSLELTRDVLRGLGLPEGEIRFTVDTFKEHDEALLYEN
                     YAHYTDTQKVATLAMKRSDELAQVLAEDWEVAAARRAQRLQDKRKADAKPSLLRVDRK
                     TPAPPSQKKPLRKSG"
     gene            2856..>3227
                     /locus_tag="VNR65_09445"
     CDS             2856..>3227
                     /locus_tag="VNR65_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HWJ18934.1"
                     /translation="MGKGIATYSGPYQTLRPQMANTLTIFLAFLRLGCTSFGGPIAHL
                     GYFRSEFVNRRKWLSDEAYADIVALAQFVPGPASSQTGFALGLMRGGLPGGLAAWTGF
                     TLPSALLMLLAAYGLSYAESSA"
BASE COUNT          585 a          958 c          949 g          735 t
ORIGIN      
        1 gaagccaacg tgccctacga cgaggtgttc gagctcgagg acatcaattc cgagttcgcc
       61 caggccgacg tcgccttcgt gatcggggcc aacgacgtca ccaatccggc ggccgaggac
      121 gacaagacct cgccgatcta cggcatgccg gtgctgcagg tttggaaggc cggcaccgtg
      181 atgttcatca agcgctcgct ggcttcgggc tatgccggca tcgacaatcc gttgttctat
      241 cgcgacaaca ccatgatgct cctcggcgat gcgaagaaga tgaccgagca aatagtgaaa
      301 gcattgtaaa gccaaccggg cgcggccgtt ttgatggcgg ctgcgccttg caatccccgt
      361 cagatggcgg tggaaagggt tgaattaagc tgacgcgacg cttgtcagag ttgtcagtct
      421 tctttacgtt ccggcgcaag aattccgtac agctaatatg aaacttgggg gatatgatga
      481 ggttcagcta tttgatttgg ctcattggcg tcgcacttgc tgtcgtcgtg ggcctttccg
      541 tgttccacgt ttatgaagtt cccgtcgcca tcgatcagct gaggcggttc ggagatgact
      601 ggtcggcaaa aacgctcttt gtcgcactgg cgcttgtggc tctgtcgaag cttttctagc
      661 tggctcgaag cttgagccaa cacggcagcg tgagtcgcca tcgtctggtt ttagttggca
      721 cctgcgatat ttcgctgcat taaggattca gagacctcta tgacgtctct ccccgatggt
      781 ttgaccggag gaaagcgcca tggatggaag cattctgatt caattggtgg tgtttttggc
      841 cgtcgcggct tttgccgcgc cggttgccaa gacccttagg ctcggttcca ttcttggcta
      901 tctgctggca ggagcgttta tcggccctta tggggccggc aaactcctcg ggtattcgcc
      961 tgcctatgcc gacacgctga gacatatcgc agagctcggc gttgcgatgt tcctgttcct
     1021 gatcgggctt gaactccgcc ttaagcgtct gtggaagatg cgcgacgctg tcttcggggc
     1081 aggtattgtc cagcttatag gaacggctgc ccttctggtc atcctgattt ggggcatcaa
     1141 tccaagttcc gatctctcct tctcgcaagc tttgctcgtg gggctggcgc tcgccttgtc
     1201 atcgaccgct ctcgttctcc agacattgga ggagaagcac gaactcacgt cgcgccacgg
     1261 ccgcctcgcc ttttcggtcc tcctgttcca ggacctcgcg gcgattccgc tgatcgccgt
     1321 cgtcccgctg cttgcggcca gcgcgccggc cggttctgcg ataaccttct cgggcgtggc
     1381 caaggccttg gcggttatcg ggctcatcat cgtcgtggga cactacgttc tcgaccggct
     1441 ttatcacgtg ctggcgtcga cccaggtgcg cgaagccatg atcgcggcgt cgctgctgac
     1501 cgtagccgga gtctcgctgc tgatgacaga gatcggtctg tccgcgtcgc ttggagcgtt
     1561 tctcgcgggt gttcttctgg ctgactcgtc cttccgccat aagctgcaag ccgacattga
     1621 gccgttcgaa atgttgctgc ttgccctgtt ttttacctcg atcggcatgt ccctgaactt
     1681 caacgccctc atcagccagc ccgggcttat cgctgccgcg gtggcgatgc tggtgtccgc
     1741 gaagatcctg gtcctctacc tgctcgggcg ttggcaacgg ctcagcgtgc cctcggcgcg
     1801 ccgctttgcc atcgtgacct cgcagggagg agagtttgcc tttgtcgtct tcgccagcgc
     1861 cgaacaaggt ggtgtgctga cgcccgggtc agcgtcagcg cttggcatca ttgtgacgct
     1921 gtcgatggtg acgacaccgc ttcttctcgc cttggacgat cttatccgca aacgcatccg
     1981 aaaggcggaa ccggtatacg acaagctgcc ctcacaagat ggccacgtag tgatttcagg
     2041 ctttgggcgg gtcggccagg tcgtagcccg catcctcaca gccaagggca ttccgttcac
     2101 agcgctcgac gccgatcccg atcaggttca actcgtcagt cagttcggca acaagagcta
     2161 tttcggcgat gccagccaac tgtctatcct ggaagccgct gagaccggca aggcgacggc
     2221 cttcgtgctt gccatcgacg acgtggagaa agcgctcaaa acggcggagc tggtgaaaac
     2281 ctattttccg cacgtcccga tttacgcccg cgcccgcaat cgccgtcatg tccatcaact
     2341 catggatctc ggcgtcaagc atatcgagcg agagaccttt gtctcgtcgc tggagttgac
     2401 acgcgatgtg ctgcgcggcc tcggcctgcc ggaaggcgag atccgcttta cggtcgatac
     2461 cttcaaggag cacgacgaag cactgcttta cgagaactac gcgcactaca cggacacgca
     2521 aaaggtagcc accctcgcga tgaagcgctc ggacgaactc gcgcaggttc ttgcggaaga
     2581 ctgggaagtg gccgcggcta gacgcgcaca gcgtcttcag gataagcgga aggccgatgc
     2641 caagccttcg ctcttgagag tggatcgcaa aacgcctgcc cccccatccc aaaagaagcc
     2701 gttgcgcaaa tcaggctgat gccgctgctc acgctggccg tcggagctgc aggcgcgtca
     2761 gcctgtgaga gttaacgatc gactaaaatt ggtttcgttt cgtaaagtgg ccataggtaa
     2821 ctatacaagt aattgtcttg tcaccaaggg cgctcatggg caaaggcatc gcgacttata
     2881 gcggtccgta tcagacgttg cgacctcaga tggcaaacac gctcacgatc ttcctggcgt
     2941 ttctgaggtt gggctgcaca agcttcggcg gacccatcgc ccatctcggc tatttcagaa
     3001 gcgagttcgt caaccgccgc aagtggctca gcgacgaggc ctatgccgac atcgtggcgc
     3061 tcgcccaatt cgtgccgggg ccggcgagtt cgcaaacggg attcgcgctg ggtctgatgc
     3121 gtggcgggct tcccggcggc ctcgctgcct ggacggggtt caccctgccg tcggcgctcc
     3181 tcatgcttct ggcagcctat ggcctgagct atgctgaaag cagcgcc
//
LOCUS       DATMGX010000581         5955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_218669, whole genome shotgun sequence.
ACCESSION   DATMGX010000581 DATMGX010000000
VERSION     DATMGX010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218669"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="VNR65_09450"
     CDS             <1..1785
                     /locus_tag="VNR65_09450"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWJ18935.1"
                     /translation="GRGEGREDAAGLAAYYNAQKGQPIWTSRDGFTQNALKVIKEIRG
                     ADDWGLDASAFQLPANPSASAKPETLADAEIKLSLAVLKYARHARGGRLDPPSLSEVI
                     DRRPHIYDPKSVLRAVAAAESSDAYLRGLHPKSEQFKRLRQALVGMNRGGGSGSSASR
                     IPAGPNFRPGDNDPQIALIRKRLGAGLDGRRDTVYDTALLQAVNRYQKEHGLRVTGLI
                     DQSLRASLNGPGDGDAGGRQAAGGGRLDERKLRILVNMERWRWMPDDLGSFYVWDSVP
                     EQVTRVFDRGKVVLMERIVVGRPTTPTPTFTASMQFVTFNPEWAVPEGIKVNEIAPKL
                     QRGGGGGGDLFNNYGGGGGSSQVLQRLGGLRVQYAGREIDPDSVDWSNVDIRRFQFIQ
                     GPGERNVLGVVKFRFPNKHDVYMHDTPDRHLFNQMPRTFSHGCMRTENPLHLAEVILG
                     HDKGVSPGQIREMVQAGTTNEIKLNSAVPVHVAYFTAEVDEQGQVHYFSDVYGLDSRV
                     ASALRGQAVHFTSDPVVTAENQDRRQAQRADPYQRSDPYQQNDPYQRNSPNQPYGPYQ
                     GWGSPSQRGGDPRYQPRRQPWNPFQDWN"
     gene            1925..2254
                     /gene="trxA"
                     /locus_tag="VNR65_09455"
     CDS             1925..2254
                     /gene="trxA"
                     /locus_tag="VNR65_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWJ18936.1"
                     /translation="MATSAVSDATFETEVKKAELPVLVDFWAEWCGPCRMVGPILEEL
                     SEEFAGQIKIVKLDVESNPSVAQTYNIRGIPALLLFKNGEVAGQQIGALPKHQLKAWI
                     TEAVASS"
     gene            2505..4076
                     /locus_tag="VNR65_09460"
     CDS             2505..4076
                     /locus_tag="VNR65_09460"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWJ18937.1"
                     /translation="MTDSSAPNTPDPEKKLSKDSVMLSQMRKRGTIYVSALALALTAA
                     LGTASYQLRPERAFAESPSVTQQKLPSFAPLVKEVKPSVVSIRVKSDVDSKVAFNGDD
                     EQQFGGNENPFEGTPFERFFNGQKSPFKHFKTPNGGHHTVMAQGSGFFISPDGYIVTN
                     NHVASDAVSLEVVMDDGKIYTAKVVGTDPRADLALLKVDGRTDFPYVKFSHSKAEVGD
                     WVVAMGNPFGLGGTVTAGIVSAHGRDIGEGPYDDFLQIDAPINKGNSGGPTFNQDGEV
                     IGVNTAIYSPSGGSVGIAFDIPAATAERVVTALKDHGHVTRGWLGVQIQGLTPELADS
                     LGLKKVTGALVATPQDGSPAEKAGIKAGDLITELNGKEVKDARDLAKKVAELAPGSEA
                     NVTLNRNGETKTLSVKIGELPEKPVQHASLRPEPSKTGMTDLGIRVAPASEVSADENK
                     GLAVVGVESGGKAAEAGLTEGDIILKVGDETVNGVQDLRRALSDAGKAGRKNALALVK
                     RNGDQRFIALPASVG"
     gene            4273..5229
                     /gene="htpX"
                     /locus_tag="VNR65_09465"
     CDS             4273..5229
                     /gene="htpX"
                     /locus_tag="VNR65_09465"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HWJ18938.1"
                     /translation="MNYFRTMLLLAALTALFMAIGYALGGGTGMTIAFVIAAGTNLFA
                     YWNSDKMVLAQQDAQEVDEKSAPELYSIVRQLAANANLPMPRVYIIHSEQPNAFATGR
                     NPSNAAVAASAGLLRMLSKEEVAGVLAHELAHVNNRDTLTMTVAATIAGAISTLANFG
                     MFFGGRRDNEGGGGPGIVGTLLAVIVAPLAAMVIQMALSRTREYGADHDGAEIVQRPL
                     WLASALRKIDNAARQIPNEQAEAHPASAPLYIINPLHGGGMDNLFSTHPNTDNRIAAL
                     VAQAQQMGQTQEPSAARMNVRLDESAPGSWGQGGPSGGGRWR"
     gene            5405..>5955
                     /locus_tag="VNR65_09470"
     CDS             5405..>5955
                     /locus_tag="VNR65_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWJ18939.1"
                     /translation="MTSHTEISGLQARQLASHLLNAVLRNKQPFDDAFAASSAKAASS
                     GLAFRDRAFARAIAATALRRLGQLEDMLARFLEKPLPADAFEARFILIAGAVQLAFME
                     VAPHAAIGLAVEQAKSTRAARHLAGLINAVLRRVNADKAAILAEQDASKLNTPAWLWQ
                     RWVRHYGEETASRIAAAHLEDPPL"
BASE COUNT         1237 a         1780 c         1819 g         1119 t
ORIGIN      
        1 ggcagaggcg aaggccgcga ggatgcggcg ggattggccg cgtattacaa tgcgcagaaa
       61 ggccaaccca tctggacctc gagggatggc ttcacccaga atgctctgaa ggtcatcaag
      121 gagatccgcg gcgcggacga ctggggattg gacgcttcgg ctttccagct cccggcaaat
      181 ccgagcgcga gcgcaaagcc ggagacgctg gccgatgctg aaatcaagct gagccttgcc
      241 gttttgaaat acgctcgcca tgcgcgcggc ggccggctcg accctccttc tctcagcgaa
      301 gtcatcgaca ggcgccccca tatctacgat ccgaaatcgg tgctgcgagc ggttgccgct
      361 gctgaatcaa gcgatgccta tctgcgcggc cttcatccaa agtccgagca gttcaagcgc
      421 cttcggcaag cgctcgtcgg aatgaacagg ggcggcggaa gcggtagttc cgcgtcgcgg
      481 attcccgctg gcccaaactt caggccgggc gacaatgatc cacagatcgc ccttatccgg
      541 aagcggctcg gggcgggcct cgatggcagg cgggacaccg tctacgacac cgcactgctc
      601 caggccgtga accgatatca gaaagaacac gggctgcgcg tcaccggcct tatcgaccaa
      661 agcttgcgag cttcactgaa tggaccaggg gacggagacg cgggcggccg gcaagcggcg
      721 ggtggcgggc ggctggatga aagaaagctg cgtattctcg tcaacatgga gcgctggcgc
      781 tggatgccag acgacctcgg cagcttttac gtttgggaca gcgtccccga acaggtcacc
      841 cgggtctttg atcgcggcaa ggtggtgctg atggagcgga tcgtcgttgg gcgcccgacc
      901 acgccgaccc cgactttcac cgcaagcatg cagttcgtga ccttcaaccc cgaatgggcc
      961 gtgcccgaag gcatcaaggt caatgaaatc gcgcccaagc tgcaacgagg cggaggcgga
     1021 ggcggcgact tgttcaacaa ctatggcggc gggggaggaa gcagtcaggt tcttcagcgc
     1081 ctgggcgggc tcagggtcca atacgccggt cgtgagatcg atccggattc ggttgattgg
     1141 tcgaatgttg atatccggcg ctttcagttc attcagggac cgggcgagcg caatgtgctc
     1201 ggcgtcgtga agtttcgctt ccccaacaag cacgacgtct acatgcacga tacgccggat
     1261 cgccacctgt tcaaccagat gccccgcacc ttcagccatg gctgtatgcg cacagagaat
     1321 cccctccatc tggcggaagt gatcctcggc cacgacaagg gcgtctcgcc tggccagatc
     1381 cgcgaaatgg ttcaggccgg taccacgaat gagatcaagc tcaacagcgc tgttccggtt
     1441 catgtggcct acttcacggc cgaggtcgac gagcaggggc aggtgcatta tttttccgac
     1501 gtgtatgggc tcgatagccg cgtcgcgagc gcgctcagag ggcaggccgt tcacttcacc
     1561 tccgacccag tggtcacggc cgagaaccag gatcgccggc aggcgcagcg cgccgacccc
     1621 tatcaacgca gcgaccccta tcagcagaat gacccctatc agcggaacag ccccaaccag
     1681 ccttacgggc cttaccaggg ctggggctct ccctctcagc gcgggggtga tccgcggtac
     1741 cagccccgtc ggcagccgtg gaatcccttc caggattgga actgatcgag cgcaagctcg
     1801 gcgtcaatta ggtgtgtcaa cgcgagcaca actattgggc ctatcttgac gctggtgctt
     1861 tcgcttccct actcttcgca gattggaatc agcggctcgg tgccgcgcaa cataagaggg
     1921 ctttatggcg acatcagccg tgtccgatgc aaccttcgaa accgaagtca aaaaggctga
     1981 gctgcctgtg ctcgtcgatt tttgggccga gtggtgcggg ccttgccgca tggtaggccc
     2041 catccttgag gaactctccg aggagttcgc gggccagatc aagatcgtga agctcgatgt
     2101 cgagagcaat ccgtcggttg cgcaaaccta taatattcgc ggcattccgg cgctcttgct
     2161 gttcaagaat ggagaggtgg ccggacaaca gatcggagcg ctgccgaagc accagctcaa
     2221 ggcctggata accgaggcgg tcgcatcgtc gtaaatccaa tctcgtatag atcggttgga
     2281 attcaaatat gccgccccat tcgtggggcg gcatattatt ttgtagattg ctcaatcatt
     2341 ccaaatatcc attttaagct gcatgccctc aagctttcat atatttaatc gaagacacgg
     2401 cttcacaacg aagcttagca acccagatct ctgtcaagtc aacacgcggc gtaacccttg
     2461 gaattgaagg ttctggccgc catcattgtc atgcggaggt cacgatgacg gatagttcag
     2521 cgcctaacac gccggatccg gaaaaaaagc tttctaaaga ttctgtaatg ctttctcaaa
     2581 tgcgtaaacg gggcacgatt tacgtttcgg cattggcgct cgcgctcacg gcagcgcttg
     2641 ggacagcgtc ttaccagctc cggcctgagc gggcttttgc agagagccca agcgtaacgc
     2701 agcaaaagct tccaagcttc gctccgctcg tgaaggaagt gaagccctcc gtggtgtcca
     2761 tccgcgtcaa gtccgatgtc gactcgaagg tcgcgtttaa tggagacgac gagcagcagt
     2821 tcggtggaaa cgagaacccc ttcgagggaa cgcctttcga acgcttcttc aatgggcaga
     2881 aatcgccatt caagcatttc aagacgccaa atggcggcca tcacaccgtt atggcccagg
     2941 ggtcgggctt cttcatctcg cctgacggat acatcgtcac gaacaatcac gttgcgagcg
     3001 acgcggtcag ccttgaggtc gtgatggacg acggcaagat ttacaccgcg aaggtcgtcg
     3061 gcactgatcc acgagcggat cttgctttgc tgaaagtcga tgggcggacc gatttcccct
     3121 atgtgaaatt ctcacattcc aaggctgaag tgggcgattg ggtcgtggcc atgggcaacc
     3181 cctttggtct cggcggcacc gtgacggcgg gcatcgtttc agcccacggg cgcgatatcg
     3241 gagaagggcc ctatgacgac ttcctgcaga tcgatgcgcc gatcaacaaa ggaaactcgg
     3301 gcggcccgac cttcaaccag gacggtgagg tgatcggcgt caatacggcg atctactctc
     3361 cctcgggagg ctcggttggc attgctttcg acatcccggc ggccacggcc gagcgtgttg
     3421 tcaccgcgct gaaagaccat ggccacgtca cgcgcggctg gcttggtgtg caaatccaag
     3481 ggttgacgcc ggaattggcc gatagccttg gcttgaagaa agtgaccgga gcgctcgtgg
     3541 cgacgccgca ggacggcagc cccgccgaga aagcagggat caaggcaggc gatctcatca
     3601 ccgagctgaa tggcaaggag gtgaaggacg ctcgcgatct cgcgaagaag gttgcggaac
     3661 tggcccctgg ctccgaagca aatgtcacgc tgaacaggaa cggcgagacg aagaccctct
     3721 ccgtcaagat cggcgagttg cccgaaaagc ccgtgcagca cgcaagcctt cgtcccgagc
     3781 cttcgaaaac cgggatgacg gatctcggca tccgggtcgc tccggcaagc gaagtgagcg
     3841 cggatgaaaa caagggtctg gccgttgttg gtgtggagtc tggcggcaag gcagccgagg
     3901 ccggtcttac cgagggcgac atcatcctga aggtcggcga tgagaccgtg aacggtgtcc
     3961 aggacttgcg ccgcgccctc tccgatgccg ggaaggcagg ccgtaagaac gcgctcgcgc
     4021 tcgtgaagcg gaatggagat cagcggttta tcgcgcttcc cgccagcgtg ggctaacact
     4081 atcactgaag gggaaagccg ctcttttcgt gacgaagatc ccgcatgtgg tccaatgggc
     4141 cgtatgcggg attttttttg cgtgataaga ttagaccata aaccgggggg cttgcgctgc
     4201 ttctccggca gaaaatattc attcgtgttt tcactgatcc gcaacgaacg ggatcaaaag
     4261 gcaggacgcg ccatgaatta cttccgcacc atgctcttac ttgccgcgct gaccgcgctt
     4321 ttcatggcca tcggctacgc cttgggcggc ggcaccggca tgacgatcgc gtttgtgatc
     4381 gccgctggaa caaacctgtt cgcctactgg aactccgaca agatggtgct ggcccagcag
     4441 gacgcgcagg aagtggatga gaagagcgcg cccgagcttt attcgatcgt gcgccaactc
     4501 gcggcgaatg cgaatttgcc gatgccgcgc gtctatatca tccactccga gcagccgaat
     4561 gcgttcgcca cgggccgcaa tccgagcaac gcggccgtgg ctgcctccgc cgggcttctg
     4621 cgcatgctga gcaaggaaga ggttgcgggg gtactggccc atgaacttgc ccatgtgaat
     4681 aaccgcgata cgctcaccat gaccgtggcg gcaaccatcg cgggcgcgat ctccacactc
     4741 gcgaatttcg gcatgttctt cggcgggcgt cgcgacaacg aggggggcgg cggccccggg
     4801 atcgtgggca ctctgcttgc cgtcattgtg gcgccgctcg cggccatggt catccagatg
     4861 gcgctcagcc gtacgcgcga atacggcgcc gatcatgacg gcgcagaaat tgtccagcgg
     4921 ccgctgtggc tggcctcggc gcttcggaag atcgacaacg cggcacggca aatcccgaac
     4981 gagcaggctg aggcgcaccc cgcgagcgcg ccgctttaca tcatcaaccc gcttcacggc
     5041 ggcggcatgg acaacctctt ctcgacgcac ccgaacacgg acaaccggat cgcggcgctc
     5101 gtcgcgcagg ctcaacagat gggccagacc caggagccga gtgccgcacg gatgaatgtg
     5161 cgacttgatg aatccgcacc tgggtcttgg ggccaaggcg gaccatccgg cggcgggcgt
     5221 tggcgctaag tcatcccgga agccgcgtgc ggctatccgg gatcgtcgca aacgcaggtt
     5281 tcgacctcta gcacttagaa tcgatcaact tttccgcctt taatcgatac caattaaagg
     5341 cgggttgatc taaagatccc ggataacgcc cggagcgttt ccgggatgac ggtgaatacc
     5401 ttcgatgaca tcccacaccg aaatttcagg gctgcaagcg cggcagctcg cctctcatct
     5461 cttgaatgca gtcttgcgaa acaaacagcc cttcgatgac gccttcgcgg catcgagcgc
     5521 gaaggctgcc tcttccggcc ttgcctttcg cgaccgcgcc ttcgcccgcg ctatcgcggc
     5581 aaccgcattg cgcaggctcg gccagctcga agacatgctc gcccgctttc tcgaaaaacc
     5641 tctgcctgcg gatgccttcg aggcgcgttt catcctcatc gccggtgctg tgcagcttgc
     5701 tttcatggag gtcgcgccgc acgcagcaat cggcctcgcg gtggagcagg cgaagagcac
     5761 ccgagccgcg cgtcaccttg cgggcctcat caatgcggtg ctgcgccggg tgaacgctga
     5821 caaggctgcg attctcgcgg agcaagatgc aagcaagctc aacacgccgg catggctgtg
     5881 gcagcggtgg gttcggcact acggcgaaga aaccgccagc aggatcgcag ccgcgcatct
     5941 cgaagatccg ccgct
//
LOCUS       DATMGX010000582         2860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_97416, whole genome shotgun sequence.
ACCESSION   DATMGX010000582 DATMGX010000000
VERSION     DATMGX010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97416"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VNR65_09475"
     CDS             complement(<1..404)
                     /locus_tag="VNR65_09475"
                     /inference="COORDINATES: protein motif:HMM:NF025079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HWJ18940.1"
                     /translation="MTRQQILTGTQLAALFDPPTDQRELVRHYTLSATDLAMIQRCRG
                     DQNRLGYALMLGYLRHPGRPLKTNERPPSALVSLVASQIDAPPEALDEYLAEERNRQR
                     HAIELQDKLGLRPFGTRPAAELLVWLLPHGIE"
     gene            complement(558..1838)
                     /locus_tag="VNR65_09480"
     CDS             complement(558..1838)
                     /locus_tag="VNR65_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HWJ18941.1"
                     /translation="MNRLITSTAIPARELKIEALASMSASFERLCLAAGLEALGEMME
                     VDARTACGPRHGRGKDRTAHRWGKTKGKIGFHGGKVEIARPRLRSFDGKEQALPSWKG
                     AMTEDWLGKWAMNQMLINVSTRKFERSMRLPEGDVPAPNGSGLSKSAASRRFVALSAA
                     RLKEWMGSDLSDLGLLVIQIDGIHMDEDMTLVAAIGVDVNGDKHPLGLVEGATENAAT
                     VQALIDNLVERGLNPTVPRLFIIDGSKALSKAIRATFGRDAAIQRCQIHKARNIINRL
                     PKSMHAQVRRVLRQAWEMDDADKAEKLLRNLARRLERDWEGVAASILEGLDEMLTVTK
                     LGLPPELRRSLACTNIIENVMGTVRRVSRNVKYWRSPSMALRWAGAAMQEAAKGFRRL
                     KAYKQLPLLQAALAVHKARATSSNAHLAQTTKAA"
     gene            complement(1908..2450)
                     /locus_tag="VNR65_09485"
     CDS             complement(1908..2450)
                     /locus_tag="VNR65_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWJ18942.1"
                     /translation="MGFPYMLVGYMRVSTDNDRQVLDLQRDALLSAGVDERQLFDDVS
                     GGQVDRPGLATLAFLRPGDCLVVWKLDRLGRSLPHLLATVNDLKSRGVAFRSLTEQMD
                     TTTPQGEFLFHVFGALAQFERSLIQERVKAGIAAARRRGRRGGRPPAVDAEKLAAMIA
                     ALDGGATKAAVWGMSRSLLK"
BASE COUNT          565 a          901 c          818 g          576 t
ORIGIN      
        1 ttttcaatcc catgtggcag cagccacacc aacaattccg cagcgggacg cgtgccaaac
       61 ggccgtagac caagtttgtc ttgtagttct atggcatggc gttgacggtt tcgttcctcg
      121 gcaaggtatt cgtcaagagc ctccggagga gcatcaatct ggctggcgac gagcgagaca
      181 agggccgatg gcggccgctc attggtcttc aagggccggc ctggatgacg cagatatccg
      241 agcatgagcg catagccgag acggttttga tcgccacggc atcgctggat catggcaagg
      301 tccgtggcgg acagcgtgta atggcgcacc aactcgcgtt gatcggtggg cggatcgaac
      361 aacgccgcga gttgggttcc agtcaaaatc tgttgtcgcg tcattcaggc ctcctgggtc
      421 tcgaggccag cactccagcc gatccgggcc agagagtcga tcagggtgct gcgattgatg
      481 ccgaaagtgc gacagagacc gcgacgatgt cccgcttgat gttgaaaagc gcgaagcggg
      541 cgtcgctgat tgagaaccta cgcagccttc gtggtttggg caagatgcgc gttcgatgaa
      601 gtggctcttg ccttgtgaac ggcgagcgct gcctggagaa gtggaagctg cttgtaagcc
      661 ttgaggcgcc taaagccctt ggcggcttcc tgcatggccg cgcctgccca acgcagagcc
      721 atcgaaggcg agcgccagta ttttacgttc cggctgacgc gccgcaccgt gcccatgaca
      781 ttctcgatga tgttcgtgca ggcgagcgaa cgccgcagtt ccggcggcag tccgagcttt
      841 gttacggtga gcatctcgtc gagcccttcc aggatcgagg cggcaacacc ctcccagtca
      901 cgttcgagac gccgggcgag attgcgaagg agcttttcgg ccttgtcggc gtcgtccatc
      961 tcccaggcct gccgcaggac gcgcctcact tgcgcgtgca tcgattttgg caagcgattt
     1021 atgatgttgc gggccttgtg tatctgacaa cgctgaatgg ccgcgtcgcg cccaaacgtc
     1081 gcccggatcg ccttggaaag agccttcgag ccgtcgatga tgaagaggcg cggcacggtg
     1141 ggattgaggc cgcgctcgac gagattgtcg atcaacgcct gcacggttgc cgcgttttcc
     1201 gtggcgccct cgaccagccc gagcggatgc ttgtcgccgt tcacatcgac gccgatcgcc
     1261 gcgacgaggg tcatgtcctc gtccatgtga atgccgtcaa tctggatcac cagcaacccg
     1321 agatcggaaa gatcggaccc catccattcc ttcagccgcg ccgccgacag cgcgacaaaa
     1381 cgccgggagg cggccgattt cgacagacca gagccattgg gcgccggtac gtcaccttcg
     1441 ggcaagcgca tcgaacgttc gaacttgcgc gtcgagacgt tgatcagcat ctggttcatg
     1501 gcccatttac cgagccagtc ttccgtcatc gcccctttcc aactgggaag ggcctgctcc
     1561 ttgccgtcga agctacgaag ccgcggacgc gcgatctcca ccttgccgcc gtggaaaccg
     1621 atcttgccct tcgtcttgcc ccagcgatgc gccgtccgat ccttgccacg cccatggcga
     1681 ggaccgcacg ctgttcgcgc atcgacctcc atcatctcgc caagcgcctc aagcccagca
     1741 gcaaggcaaa gcctctcgaa gctcgccgac atgctcgcca gcgcttcgat cttcaactct
     1801 ctcgcgggta tcgcagtgga tgtgataagt ctgttcatag cgttgctttc ctctcgtgga
     1861 atcagcaccc cgagcctaca ggctcgaggc gagcaacgcc gcttcttcta tttcaacaat
     1921 gatcgggaca tcccccagac cgcagccttt gtggcgccgc catcaagagc cgcgatcatc
     1981 gccgcgagtt tctcggcatc gacggcaggc gggcgacctc ctcgccgccc tcgccgcctg
     2041 gcggcggcga tgccggcctt cacccgctcc tggatcaagg atcgctcgaa ctgcgccagc
     2101 gcgccgaaga cgtggaacag aaactcgccc tgaggggtgg tggtgtccat ctgctcggta
     2161 agcgaacgga atgccacacc acgcgacttg aggtcattca ccgtggccag aagatgcggc
     2221 agcgaccgac cgaggcgatc cagtttccag acaaccaaac aatctcccgg ccggaggaag
     2281 gctagagttg cgagaccggg gcgatcgacc tggccgccgc tgacgtcatc gaacagctgg
     2341 cgctcatcaa ccccggcgga aagaagcgcg tcacgttgaa ggtccagcac ctgccggtca
     2401 ttgtcggtgg aaacgcgcat atagcccacc aacatgtacg gaaaacccat caggagccgt
     2461 ttccgtatac tatctcattc cgacatgagt ttccgtgcat attttcggcc cctggcaagc
     2521 cgtttcagcc gcttctgcag cctgccggaa aacaagtgtt ttccgttatc gccatttccg
     2581 cctcatccaa accgtcgaaa acctgcaacc ctttgctttt gctagaaaac taaatctgcc
     2641 gcgttcccct tatgtctgct tatgtgtacc agatgggcgt ttcattagtt gaggccctca
     2701 tggaaaccca aatcgtcgcg caatcctgcc cggccgaaac cattgcgcat aaatggcatg
     2761 tcttacacga cattcggaaa gccaaggacg cgattggcgt ttcagaccgg tccatcgcgg
     2821 ttctcaacgc tcttctttcc ttccaccagg aaacgaccct
//
LOCUS       DATMGX010000583         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_344577, whole genome shotgun sequence.
ACCESSION   DATMGX010000583 DATMGX010000000
VERSION     DATMGX010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344577"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /locus_tag="VNR65_09490"
     CDS             complement(<1..2003)
                     /locus_tag="VNR65_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWJ18943.1"
                     /translation="MKFNLSQWALKQRSFTIYCMIALVIAGTMSYLRLGRNEDPAFTF
                     RTMVVFAGWPGADLNETLKQVTERLERTLQETPSLDHVESYTKAGRTTIFVTLKGETP
                     PSKVPDIWYHVRKSVADMRHTLPAGVVGPGFNDEFGDTYGIIYGFTADGFSHRELRDS
                     VEDIRSKLLQVPDVSKIEILGAQDEKIFVEFSTEKLAGLGVDRAALAAALEAQNIVVP
                     AGTIQTGNEAFALRTTGAFDSEKDLENVTFVVNGRLIRLRDIATVRRGLADPPQPMFR
                     VNGEPAIGLAIAMREGGNILVLGRNIEKAMKEIKADLPLGVEAFLVADQPQTVKHAIG
                     DFMTSLWQAIAIVMAVSFVSLGVRAGTVVALSIPLTLAVVFPIMAMIGIDLQRISLGA
                     LIIALGLLVDDAMTTVDVMSSRLAQGDKKEDAASFAYTSLAFPMLTGSFVTAAGFVPI
                     GFAKSSAGEYTFSIFAVVSIALIVSWFVAVLYTPLLGVWLLKAPEKKETNKPGRIMQA
                     FRSLLVGAMRARWLTIGITLGCFVAALLASPFVARQFFPPSDRPELLVDVRLPQNASI
                     YASEDVATKFDELLKGDPDVKSWSTYVGRGAIRFYLPLNVQLANDFFSQAVIIAKDLE
                     ARDRLQKKLETSLPEKFPSAISRVTPLELGPPVGWPVQYRVSGP"
     gene            complement(2003..>2511)
                     /locus_tag="VNR65_09495"
     CDS             complement(2003..>2511)
                     /locus_tag="VNR65_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWJ18944.1"
                     /translation="GRTIVQVARDGGRDAVFNVPASVLRAAPGDTEVTVSLADDPKVQ
                     ATGRVREVAPQADPVTRNFQVKVGLEDPPAAMRLGSTVTGNARLQSAPVIAVPASALT
                     KYDNKPAVWVVDKSNQTVAIRNVEVKRHEPSSVVISNGLESGDIVVTAGVQALYPGRK
                     VRLLGVSS"
BASE COUNT          510 a          744 c          768 g          489 t
ORIGIN      
        1 gggccgctga cacggtattg aacaggccat ccgacgggtg ggccgagttc aaggggcgta
       61 acccgcgata ttgcgcttgg gaacttctcg ggcaaggatg tttcgagttt cttttgaagg
      121 cggtcccgcg cttccagatc cttcgcaatg atgaccgcct gggagaagaa gtcgttcgcg
      181 agctgaacat tcaggggcag gtaaaagcga atcgctcccc ggcccacata ggtgctccag
      241 cttttgacgt caggatcacc cttcaagagt tcgtcgaact tggtagcaac gtcttcgctg
      301 gcatagattg aggcgttctg cggaagtctg acatcgacga gaagttcggg ccggtccgat
      361 ggcggaaaaa actggcgcgc gacaaagggg gaggcaagaa gcgccgccac gaagcagccg
      421 agcgttatgc cgatcgtcag ccagcgcgct cgcatcgctc cgacaagcag gcttcggaaa
      481 gcctgcataa ttctgcccgg cttgttcgtc tctttttttt ccggcgcttt gaggagccag
      541 acgccaagaa gaggtgtgta aagaaccgcc acgaaccagg aaacgatcag cgcaatgctg
      601 acgaccgcaa agatcgaaaa ggtgtattcg cccgccgagc ttttcgcgaa accgataggc
      661 acgaaccctg ctgcggttac gaaagatccg gtcagcatcg gaaaagccag cgacgtgtag
      721 gcgaagctcg cagcatcctc cttcttatcc ccctgcgcga gccgggatga catgacgtcg
      781 accgtcgtca tggcgtcgtc gacgaggagg cccaaggcaa tgatcaacgc gccgagcgaa
      841 atgcgctgga gatcgatccc gatcatggcc atgatcggga agacaacggc aagcgtcagg
      901 gggatcgaaa gcgccacaac ggtgcccgcc cggacgccga gtgatacgaa gctcacagcc
      961 atgacaatgg caatggcctg ccagagcgac gtcatgaaat cgccgatggc gtgtttcacg
     1021 gtttgcggct ggtcggcgac gaggaacgcc tcgacgccga gcggcagatc ggccttgatc
     1081 tccttcatcg ccttttcgat gttccggccg agcaccagga tgttgccgcc ttcacgcatt
     1141 gcgatggcaa ggcctattgc gggttctcca ttgacacgga acatcggctg cggaggatcg
     1201 gcgaggccac ggcgaacggt cgcgatatcc cgaagccgga tcaggcgtcc gtttacgacg
     1261 aaggtcacat tctcgaggtc tttctcagag tcgaatgcac ccgtcgttcg aagcgcgaaa
     1321 gcctcgtttc ctgtctggat ggtcccggcc ggaacgacaa tattctgcgc ttcgagcgct
     1381 gcggcaaggg cggcccggtc gactcctaat ccggcgagtt tctcggtcga gaactctacg
     1441 aagattttct catcctgcgc gcctaggatt tcgatcttcg atacgtcggg cacttgcagg
     1501 agtttcgagc gaatatcctc gacgctatcc cgaagctcgc gatggctgaa gccgtcggcg
     1561 gtgaagccgt agataattcc gtaggtgtcg ccgaactcat cgttgaaacc ggggccgacc
     1621 acgccagcgg gaagcgtgtg gcgcatatcg gccacactct tgcgaacgtg gtaccagatg
     1681 tccggaactt ttgagggcgg cgtctcgccc ttgagcgtga cgaaaatcgt ggtgcgcccc
     1741 gccttcgtat aactctcgac gtgatcgagg ctcggcgttt cctggagcgt ccgctcaagc
     1801 cgctcggtca cctgtttcag cgtctcgttc aggtccgcgc ccggccaacc cgcaaacacg
     1861 accatggtgc ggaaggtgaa ggccgggtct tcgttccggc caaggcggag gtaggacatc
     1921 gtgcccgcaa tcacgagcgc gatcatgcag tagatcgtga aggaccgttg cttgagcgcc
     1981 cactgcgaga gattgaattt catcaggagg agaccccaag cagccggacc ttgcggcccg
     2041 ggtacagggc ctgaacgccg gcagtcacca cgatgtctcc actttcgagc ccgttggaaa
     2101 tgacgacaga cgacggctcg tggcgcttga cctcaacatt tcggattgca acggtctggt
     2161 tcgatttgtc gaccacccag accgcaggct tgttatcgta cttcgtaagg gcgctcgccg
     2221 ggacggcgat cactggggcc gactgcagcc gcgcgtttcc tgtcacggtt gaaccgagcc
     2281 gcatggctgc gggaggatct tccagcccca ccttcacctg gaagtttctc gttacagggt
     2341 ccgcttgcgg agccacctcg cggacgcggc cggtcgcctg cacttttgga tcgtctgcaa
     2401 ggctcacggt gacttccgtg tcgcccggag cggccctcaa gacgcttgca ggcacgttaa
     2461 aaacggcatc gcgtccgccg tcgcgcgcca cttgcacgat cgtacgtccg g
//
LOCUS       DATMGX010000584         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_208624, whole genome shotgun sequence.
ACCESSION   DATMGX010000584 DATMGX010000000
VERSION     DATMGX010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208624"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VNR65_09500"
     CDS             <1..600
                     /locus_tag="VNR65_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HWJ18945.1"
                     /translation="PRLPVLADGADHRSWYARSRSRVATAILLTAPGIPQLFMGQEFL
                     EDKPWDVLPTGPNLLSWESLDASADSARRDHLQFTKDLIRLRRDLPALRGDYVRAFFC
                     SDHDRVIAWHRWREGTGDDVIVVASFAETTWSGYEIGFPYPGVWQERFNSDAYDHFVN
                     PIVAGNGGGVVAVGRGLHGFAASAGIVIPANGVVVFARG"
     gene            756..1016
                     /locus_tag="VNR65_09505"
     CDS             756..1016
                     /locus_tag="VNR65_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18946.1"
                     /translation="MLKLAAAAILLLSSPAFAQTSSVDCPPTGQTAKGELVYGMDCKA
                     LKAENRAENRPNMPPTNMKDTVIPKSGATQNPESTPTTGVNK"
BASE COUNT          471 a          707 c          684 g          439 t
ORIGIN      
        1 ccgcggctgc ctgttctcgc cgacggtgcc gatcaccgct cctggtacgc gcgcagccgc
       61 agccgcgtgg ccaccgcgat cctgctgacc gcgcccggca ttccccaact cttcatgggc
      121 caggaattcc tggaggacaa accgtgggac gtgctgccga cggggccgaa cctgctctcg
      181 tgggaaagcc tcgatgcttc agccgattcc gccaggcgcg atcacctgca attcacaaaa
      241 gacctgatcc gcctgcgccg cgatctgccc gcgctgcgcg gcgactacgt ccgcgccttc
      301 ttctgctccg accatgaccg cgtgatcgcc tggcaccgct ggcgcgaggg caccggcgac
      361 gacgtcatcg tggtcgcctc cttcgcggag acgacatgga gcggctatga aatcggattt
      421 ccctatccgg gcgtctggca agagcgcttc aacagcgatg cctacgacca ttttgtgaat
      481 ccgatagtcg cgggcaatgg cggcggcgtg gttgcggttg gcaggggcct gcacggcttt
      541 gccgcgtcgg cgggaatcgt gattccggcg aatggggtgg tggtgtttgc gagggggtag
      601 gcggattgcc agcgcactgc acacaccgtc agcttgcgtc gctctgccaa cgataacggc
      661 tttcgcaggc ctttgccgcg gaaagtggtt cccgagggaa cttgtagccc cgacgtctgt
      721 tgcctccaac acaatcataa atcctggagg aaacaatgtt gaagctggcc gccgccgcaa
      781 tcctgctgct ctcgtccccc gccttcgcgc aaaccagctc ggtcgactgt ccaccgacag
      841 gacaaaccgc aaaaggcgaa ttggtttacg ggatggattg caaggcgctg aaggcggaga
      901 accgcgcgga gaatcggccc aacatgccgc cgacgaacat gaaagacacg gttattccga
      961 agtcgggtgc aacgcagaat ccggagagca ctccaacaac cggcgtgaat aaataaatca
     1021 aacccccggt ctggctggga cggaccgtgg gattggcgaa accgtcatga ccggaagaac
     1081 caaaacgaaa ctaaagggca tcgctgcccg gaaagctcaa acgcgattag accgtgagct
     1141 gagagcccta agccgcgctg caggcgagac ctcgcatggc gaagcgaacg agacccggac
     1201 cgagagtagt ccgggtctct aacaaacacc ggaacagctc cctcatgccg ctcggcaaat
     1261 gctccgatga acggtagtgc ctagatcacg tgccgcgttg atctaggaca agccgcaacg
     1321 cgcgagtatt gaaaaggtaa aaccgcacgg ccccattggg gctgggaaac agaaccgcgc
     1381 ggtcgcccta cgcccatcga aagcataggc aaggccatca tgaccgggag tgggattgcc
     1441 gcctgtgcaa tatcgctcac cgcgaggata tttctggcgc gcgcaccctc tacttggttg
     1501 ccagccatcg gttcatctcg gcggccttgc gcgataacct ccgtcgctcg ttcgtcccaa
     1561 gcgcggctat caggtgggcg ctccgcatct tgctccacgg tcgttactcc gaacgctttg
     1621 acgtccatca cctcttactt cggcgcgaat aattggcctt ggagccaatc cattgtcgct
     1681 ttggactgca tggggtcact gagcctcacc gccgtcttaa gttgatgcga gcaacgacga
     1741 cattcaaagg tccgaaggtc ataacctgac ggaccttctt ggatgcgtcc taggagcatt
     1801 tcggattggc actttgaaca gattatcttc cggaactggg gcatgatgct catccctatg
     1861 atctaggcgg gagcacaaca ctctctgtca ccggaatttg cctagtggtg tagcggtgat
     1921 gcagccagca ttgaacatgg agttgcgatt cgctggtcag tattgatcgg tctccgacag
     1981 atgatgaaat atgttcaaca cgcctcttct gcagtcgtcg agattttacg caatttgagt
     2041 gcagccgcca gcgcatccgc ctgccgactt gtccacaccc acgagcggcg acggctcgcc
     2101 tccgttccaa gcgcaaccca tggcgtgatt cgcagagcgg gtcagctcgc gctttgatgc
     2161 gcgcatgtcc gacacccgca cgatctccca gcctgaaggc gaaggctatc cgtggatcgg
     2221 ttgcgagtgc tgcaagagca cggtgtgggt gccggcgcgg atttcacctt cggcggcggc
     2281 cgtcacggcg gcgaggccga g
//
LOCUS       DATMGX010000585         3140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_287851, whole genome shotgun sequence.
ACCESSION   DATMGX010000585 DATMGX010000000
VERSION     DATMGX010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287851"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VNR65_09510"
     CDS             complement(<1..886)
                     /locus_tag="VNR65_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWJ18947.1"
                     /translation="MSYAVAIALLLLLGLLTLVSYVDRVYQEVGKFLSREFQDNIDVF
                     EQKVEPELGVGRTRAALSMAVLTQLTMAAIALLIGFIVFTDLTWNIYEILQTTISLIL
                     IVIVFNRFLPFVFFSRTKGEWLIHWTLVLKVLIYTVMPVTIALGFLQSVAALTKQNTG
                     EQPETSAEAVDALIEAGQEEGIIQEGDRDLIQSVVEFSGKTVREAMKPRPQMFAVSSD
                     TTVERFIEMLRVKHYSRVPVYEGSIHNIKGIVYTQDVLQVPDTEAHVRVLETIMRRDV
                     YFVPESKLGSDLLREMQKQ"
     gene            complement(883..1380)
                     /gene="ybeY"
                     /locus_tag="VNR65_09515"
     CDS             complement(883..1380)
                     /gene="ybeY"
                     /locus_tag="VNR65_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWJ18948.1"
                     /translation="MVILQKKVAGLSASTLERFVVRARKAVGLRSPVNVLVTSSSALR
                     SLNRQFRSQNKATDVLSFPPPSPSTASRNKPSLAGEVAISADIALQNSIRLGHPVAQE
                     IKILALHGILHLAGFDHEDDNGEMARKEQTLRRALKLPAALIERVQATNQRGARRATG
                     ARRMA"
     gene            complement(1453..2454)
                     /locus_tag="VNR65_09520"
     CDS             complement(1453..2454)
                     /locus_tag="VNR65_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWJ18949.1"
                     /translation="MRLLEDGLNITINLRSNAIELEGAAKDVARVEQVFADYETLLRA
                     GHTFNNGDLNSMLRVLISDPNSTLRGLADAGRQRSFGRRTVQPKSSNQRRYLEAIEAH
                     DMVFGIGPAGTGKTYLAVAMAISALLAKRVNRIILARPAVEAGERLGFLPGTLQDKID
                     PYLRPLYDALYDMLDPEKVDRYLEKNVIEIAPIAFMRGRTLNDSFVILDEAQNTTSEQ
                     MKMFVTRLGFNSKAVITGDATQIDLPNARRSGLLEAMDVLKSVEGLAFVHFDETDVVR
                     HHLVQRIVRAYDEHKTRVAEQQMLLLAEPRHADAASAEKQAAAAAKESKSASAEAKG"
     gene            2778..2987
                     /locus_tag="VNR65_09525"
     CDS             2778..2987
                     /locus_tag="VNR65_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18950.1"
                     /translation="MKKPRLHVTKWLGEIPVEIECTSCPEWRFRAAPSSHRPNREECS
                     QQLQRAFGQHFKTVHAPEGSAKVSE"
BASE COUNT          627 a          903 c          960 g          650 t
ORIGIN      
        1 tctgcttctg catttcacgc agcaggtcgc ttcccagctt gctctcgggg acgaagtaga
       61 catcgcgacg catgatggtt tcgagcacgc gcacgtgtgc ttcggtgtcg ggaacttgca
      121 gcacatcctg tgtgtagacg attcccttga tgttgtggat gctgccttca tacaccggca
      181 cgcgcgagta gtgcttgacg cgcagcattt cgatgaaacg ctcgaccgtc gtatcggagg
      241 aaacggcaaa catctgcgga cgcggcttca tcgcctcgcg caccgttttg ccgctgaatt
      301 cgaccaccga ctgaatcagg tcgcggtcgc cttcctgaat aatgccctct tcctgcccgg
      361 cctcgatcaa cgcgtccacg gcctcggccg atgtttccgg ctgctcccct gtattctgct
      421 tggtgagcgc cgctactgac tgcaggaagc ccaacgcgat tgtcaccggc atgaccgtgt
      481 agatcaatac tttcaagacc agagtccagt gaattaacca ctcacctttg gtacgcgaga
      541 aaaagacgaa cggcaggaag cggttgaaca cgatgacgat caggatgagg ctaatcgtcg
      601 tttgcaggat ctcgtagatg ttccaggtca gatcggtgaa gacgataaag ccgatgagca
      661 gcgcaatcgc tgccatggtc agttgcgtga gcaccgccat ggagagtgcg gcccgggtgc
      721 gtcctactcc tagttccggc tcgacctttt gttcgaagac gtcgatgttg tcctggaatt
      781 cgcgggaaag gaacttgcca acttcctggt agacgcggtc tacgtaggag acaagcgtca
      841 atagtcccag caggagaagc agcgcgatgg caacggcgta actcatgcca tcctccgcgc
      901 tcctgtcgca cgtcgtgcgc cgcgctggtt ggtggcttgg actcgctcga tcaaggccgc
      961 cggcagcttg agcgcccggc gcagtgtctg ctctttgcgc gccatctcac cattatcgtc
     1021 ctcgtggtca aagcccgcca gatgaaggat cccatgcagc gccagaatct tgatttcctg
     1081 ggcgacagga tgcccgagtc ggatcgagtt ttgcagggca atatcggcgg agatggcgac
     1141 ttcgccggcc aggcttggct tattgcgaga tgccgtactc ggggatggag gcggaaacga
     1201 caacacatcc gtggccttgt tctggctgcg aaactggcgg ttcagcgaac gcaaagctga
     1261 actgctggtg acgagcacgt tcactgggga gcgaaggccg acggccttgc gcgcgcgcac
     1321 gacaaaacgc tccagcgtgc tggcactcaa tcccgcgact ttcttttgca gaataaccaa
     1381 gtccttttct ccgcgaggtg caaaagcaaa caggagggcc aagtcgcggc cctcccgccg
     1441 aatatgtatt gcttatccct tggcttcggc cgatgccgac ttcgattcct tggcggcggc
     1501 tgccgcctgc ttttcggcac tggccgcgtc cgcatgtctg ggttcggcca gcaaaagcat
     1561 ctgctgctcg gccacacgcg ttttgtgttc gtcgtaggcg cgaacaatgc gttgcaccag
     1621 gtgatggcgt acgacatccg tttcatcgaa gtgcacaaag gccagccctt ccacgctttt
     1681 cagaacatcc atggcctcga gcagtccgct gcggcgcgcg ttcggcagat cgatctgcgt
     1741 ggcgtcgccg gtaatgaccg ccttcgagtt gaagcccagg cgggtgacaa acatcttcat
     1801 ctgctccgag gtcgtgttct gagcctcatc gagaatgacg aaggaatcgt tcagcgtgcg
     1861 tccgcgcata aaagcgatcg gcgcgatctc gatgacgttc ttctcgagat agcgatccac
     1921 tttttcggga tcaagcatgt catacaaggc gtcgtacaag gggcgcaggt aaggatcgat
     1981 cttgtcctgc agagtgccgg gcagaaaacc cagtcgctcg ccggcttcga ccgcagggcg
     2041 cgccaggatg atgcggttca cgcgcttggc cagcagcgcg gagatcgcca tggccacggc
     2101 gaggtaggtc ttcccggttc cggctggacc aatcccaaat accatgtcgt gggcctcaat
     2161 ggcttcgaga tagcggcgct ggttgctgct cttgggctgg acggtgcggc gtccaaagga
     2221 gcgctggcgc ccggcatcgg ccaatccgcg cagggtcgag ttgggatcgg agatcagcac
     2281 gcgcagcatg ctgttgaggt cgccgttgtt gaaggtgtgg ccggcacgga gcagggtctc
     2341 gtagtcagcg aagacctgct cgacccgagc gacgtctttg gccgcgccct ccagttcgat
     2401 ggcattcgac cgcagattga tggtgatgtt caggccgtct tccagcaggc gcacattctc
     2461 atcccgtgtg ccgaagaagg tctcgatgtt ggggctgacg ccgatgcttt tcttcattca
     2521 gtttatttga ggaaccttat ggagacagcc gctccttcga cttcgctcaa gacaagcctc
     2581 ggctctccgg aagccgcgga acattcgaga tgcacgagtg caactcacgg acaaagcagg
     2641 gtttcgcttg gagacgggac caaccggaat gcgcatgccc agaaggcaag tcactaccat
     2701 taagttaaag cctaaccccg gggcgaacag ggagtcaatg ggagcaaaat gtagggcgcc
     2761 tgtgggaaac ttgcaggatg aagaagccga ggttgcatgt caccaagtgg cttggggaga
     2821 ttcctgtaga gatcgagtgc acatcctgcc cggagtggag gttccgcgcc gcgccctcga
     2881 gccatcggcc aaaccgggaa gaatgtagcc agcaattgca gcgcgcattt ggccaacact
     2941 tcaagacggt tcatgcgccc gaaggctccg ccaaggtcag cgagtgaccg caccgtcatc
     3001 gggaaccgaa gcgggcacag ccctggtgcg ggcccactcg cgcagggtct ggatctcttc
     3061 tgcgcgcgtg gtggagagcg ggaccgtctg catgagggcg tcgagcagcg actgcgtgga
     3121 gagttgcttc ttctcggaat
//
LOCUS       DATMGX010000586         2247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_206299, whole genome shotgun sequence.
ACCESSION   DATMGX010000586 DATMGX010000000
VERSION     DATMGX010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206299"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..529
                     /locus_tag="VNR65_09530"
     CDS             281..529
                     /locus_tag="VNR65_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWJ18951.1"
                     /translation="MLGNCRRAMRSGGRLLLVETVLPPGNVMHPGMILDMAMLAGLGG
                     QERTEAEYRALLHKAGFQLMRVVPTSSAVSVIEATVRG"
     gene            complement(718..1722)
                     /locus_tag="VNR65_09535"
     CDS             complement(718..1722)
                     /locus_tag="VNR65_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWJ18952.1"
                     /translation="MAANRRDVIALGGAALAFGVGASGTPAAARAARRWGGQHPTNLN
                     YATIESSAGGLGLGLRTQRGIVHVAAYEKAQNRGLPTTVEQLIAKDGDLEALPGLAAS
                     APAHYVRAEAAVRFGPVVSHAPKIFCVGLNYRAHILEGNPSGEVPKEPILFNKFDSCL
                     NSHNGVIPVSRQRGSQWDYEAELVMIIGTGGSNIPKAQALSHVFGYCNGNDFTERARQ
                     RTSGQWMLGKAGDGWGPLGPWLVSADQIDPTDLDLKCIVNGEVRQNSNTRLMIFDCAT
                     LVSYISENWELKPGDVIFTGTCEGVIAGYPPDKQVWLKPGDKISTEISKLGRLDFTLT
                     "
     gene            complement(1747..>2247)
                     /locus_tag="VNR65_09540"
     CDS             complement(1747..>2247)
                     /locus_tag="VNR65_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18953.1"
                     /translation="DGKRAGEFMHINPREHSLAIVAGSMIPGGNDRPQLGHIMMEMQY
                     LDDVGQSYDIVTKDYYNSLFLTLGRHNNDLMTSFYVHTPSEFLIECGWGGLLIDPTRW
                     QAEELPLVSIWGHQMMQDGKLVKSPFPPPTVRALATPLQVYGENFESNHRPTRLSQVL
                     KTEPFR"
BASE COUNT          415 a          708 c          679 g          445 t
ORIGIN      
        1 aagcttacgc cgagatagct caatagattg gcacgccggt cactgtccaa aagtcgcgca
       61 ggtttacacc gtaaacaccg ttttcgtgcg acaccgtaga gaccaggtcc aaaatcatgc
      121 gcaaactcat ctcgctaaga aacctgagga agagttccat gaacaggccc agttcgacct
      181 atgcaatcct tgcagcaacg gtggtctgcc tcgggggcgc tgctcgcgcg gaagcggcca
      241 cgtctcgtga agcccaggca ctggccagcg ccgccttacg atcctcggaa attgccggcg
      301 ggcaatgcgc agcggaggtc gactgttgct ggtcgagaca gtgctgccgc ccggcaacgt
      361 catgcatccg ggcatgatcc tggacatggc catgctggcc gggctgggcg ggcaggagcg
      421 cactgaggcc gagtatcgag cgttgctcca caaggccgga ttccaactca tgcgcgtcgt
      481 gccgacgagt tccgccgtca gtgtcatcga agcgactgtc cgtggctgac catgcagcga
      541 gcggccctgt gcgtcggatc agcccttggc acgagcttgc agtcgcctga gtgacgtcag
      601 ctggcgtgca gtgctcaagc cgggacggcc ggcagaacga cgagcggatc atcgcgcata
      661 aacgcgatgg cgcgaaatat cgctcgcctt ggttgaaagc cttacgcccg cgagacgcta
      721 ggtcagcgta aaatccagcc gtccgagttt tgatatttca gtggagatct tgtctccggg
      781 cttcaaccac acctgtttat ccggcggata gccggcgatc acgccttcgc acgtgccggt
      841 gaagatcacg tcacccggtt tgagctccca attctcggag atgtagctca ccagcgtggc
      901 gcaatcgaag atcatcagcc tggtgttgga attctgccgg acctcgccat tgacgatgca
      961 cttcaggtcg agatcggtgg ggtcgatctg gtcggcgctg accagccatg gtcccagcgg
     1021 gccccagccg tcgccggcct tgccgagcat ccattgcccg ctggtgcgct ggcgggcgcg
     1081 ctccgtgaag tcgttgccgt tgcaatagcc gaacacgtgc gacagcgcct gcgccttggg
     1141 gatattggag ccgcccgtcc cgatgatcat caccagttcc gcctcgtagt cccattggga
     1201 tccccgctgc cgggagacgg ggatcacgcc attgtggctg ttgaggcagg aatcaaattt
     1261 attgaacagg atcggctctt tcggcacctc gccgctcgga ttcccctcca ggatatgcgc
     1321 gcggtagttc aggccgacgc agaagatctt gggcgcgtgc gagacgaccg ggccgaacct
     1381 cacggcggct tccgcgcgca catagtgcgc tggcgccgaa gcggccaggc ccggcagcgc
     1441 ctccagatcg ccgtccttgg cgattagctg ctcaaccgtc gtcggcaggc cgcggttctg
     1501 agccttttca taggcggcga catggacaat cccgcgttgc gttcgcaggc caaggccgag
     1561 gccgcccgcg ctcgactcga ttgttgcgta gttcaggttc gtcggatgct gcccgcccca
     1621 acggcgtgcc gccctcgccg ccgccggcgt gcccgaggcg cccaccccga acgccagggc
     1681 ggctcccccc agagctatca cgtctctacg gttggcagcc atcggcgctc ctttgcatgc
     1741 ccgcgctcaa cggaacggtt ctgtcttgag gacctggctc aaccgggtcg gacggtgatt
     1801 ggattcgaag ttctcgccat acacttgcaa cggcgtagcc aacgcccgca cagtcggcgg
     1861 cggaaatgga gacttcacca gttttccgtc ttgcatcatt tgatggcccc agatgctgac
     1921 caagggcaac tcttccgctt gccaccgcgt cggatcgatc agcagtccgc cccagccaca
     1981 ctcgatcaag aattccgacg gcgtatggac gtaaaacgag gtcatcaggt cattgttgtg
     2041 ccgaccgaga gtgaggaata ggctgttgta atagtccttc gttacgatgt cgtaggattg
     2101 tccgacatca tccaggtatt gcatctccat catgatgtga ccaagctggg gcctgtcgtt
     2161 gccgcccggt atcatgctgc ccgcaacgat cgccagactg tgttcacgcg ggttgatgtg
     2221 catgaactcc cccgcgcgtt tgccatc
//
LOCUS       DATMGX010000587         2486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_440627, whole genome shotgun sequence.
ACCESSION   DATMGX010000587 DATMGX010000000
VERSION     DATMGX010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440627"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            882..1358
                     /locus_tag="VNR65_09545"
     CDS             882..1358
                     /locus_tag="VNR65_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18954.1"
                     /translation="MESVSKRFKTVVFEDGTHAVVRTDPGKPRLIEVVATFYDAARAR
                     DYADSENGQSPEPQPQIAPAARKLAPNATPKQDSVKPTRISLDLSQRQSAVLTALRVK
                     MNGNKFVEVKAAVLAEAANTPLGSLHSVLQSLEKKQLILTTRGGSARAPAVYQVLG"
     gene            1402..1647
                     /locus_tag="VNR65_09550"
     CDS             1402..1647
                     /locus_tag="VNR65_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18955.1"
                     /translation="MRSTQTIVEVQPRDILTASELTKVHDFLCDADMRVTEVKALFFH
                     TGDAATAARLKDIQGRLADEIRAVERLITAAKPAFRQ"
BASE COUNT          572 a          749 c          697 g          468 t
ORIGIN      
        1 tgagacgccc acatcgccac agcgattcgg gcccgcagcc aacatgcaag gggtcaggct
       61 aggcgatggt atcactcaat cgcggtgcgc gatttcatcg tccgcaatgt ttcaccctat
      121 gatggtgacg agacatttct cgtagggcca tcgaagcgca caagggcggt ctgggagaag
      181 ctccagccct tccgaggcgg cgacaggggg agcggatctc agcgagcgcg aagtggaagt
      241 gctgcggtta acctcggtgg gtcatagcaa taaggcgatc gcaaatatct taaagatcag
      301 cgtcaggagc gtcgagacct acaaggcgcg agcaatggat aagctcggct ttgacaaccg
      361 agtcgatttg gtgggattat gcggttgaga agggctggct agccggaagc tgatgaacag
      421 cgtgttgatt ggaggcgctg ggccggttgg ggtcaaggac ttccagaacg gcgacgtctg
      481 gcttaagttc gaccgccatc cctccggagg aggcccgcgc ctatgctggc ctcgcaataa
      541 agcagccccg cgagaattgc cggatcgatg ctgcctaaag agccttggct ggaggcgtcg
      601 aggcgccgaa cagagctgcc gaacagaaga gcgaatacga cgccacgata gcgcacgtca
      661 ccttacgggg actgccgtaa aaagaaagag gtcgcgtccc cagtcgcctc acgaactccc
      721 ctgaccctgt caatcactcc ccaccccata tggtgtcgtg taggtagttt ccccagcttg
      781 ccgactgatt gccttgttgt catgttacaa ctgagcgatt tttcgcttca caaggcatat
      841 tgtcgactgg cgcgatgttc gccgccgaaa aggaaatggg catggaaagc gtgagtaaga
      901 ggttcaagac cgtcgtgttc gaggacggca cccatgccgt ggtgcgcact gatcccggca
      961 aacctcgcct catagaagtc gtcgcgacgt tctacgacgc cgcacgcgcc cgagactacg
     1021 cagacagcga aaacggtcag tctcccgagc cgcagccgca aatcgcaccg gcagcccgta
     1081 aactcgcacc taatgcaact ccgaaacaag acagtgtcaa gccaacgcgc atcagcctcg
     1141 atctcagtca gcgccagagc gcggtgctaa cggcgctccg tgtcaaaatg aatggcaaca
     1201 agttcgtaga agttaaggcc gccgttctgg ccgaagcagc gaacacaccg ctggggtcgc
     1261 ttcactcggt tcttcagtcg ctcgaaaaga agcagctcat tctgaccacg cgtggaggct
     1321 ccgcacgagc gcccgcggtc taccaggtct tggggtaagc ggtccgaccg aaaggaagaa
     1381 agcgccgttg aggatgcgcc gttgcgtagc acgcagacga ttgttgaagt tcaaccccgg
     1441 gatatactca ccgcttcgga gctcacgaag gtgcacgact tcctctgcga cgcggatatg
     1501 cgcgtgaccg aggtgaaggc gctcttcttt cacaccggtg acgccgcgac ggccgctcgg
     1561 ctcaaggaca ttcaggggcg ccttgctgat gagattcgag ccgttgagcg attgatcacc
     1621 gccgccaagc cggcgtttcg ccaatgaact cgccgcccaa ggcgacaaaa aagaagccgc
     1681 gacaccaagg tgtgcggctc ataagtctag ggaggaacct agacagtgcc attcttctga
     1741 taaattatcg ggcaaagcaa tcgcttcaat tggcgatacc gcagcattcc ccgtcgggtg
     1801 tcgtaaatat gccgccctcc ctcgttcgct tctcggcgcg tgggcagcac aggcgacttc
     1861 atcaaggcat ttttcttaca ttttcaccga acacgaaatg cggctgtcgt tgtagcaaca
     1921 ggacgagttc gtctcactcg ccggctgtta tacgacagag acaaaaaaaa gcgttcaacg
     1981 acatgccgat agcgctccca ataagggctt tgatctcatc gcccatggtg cacccaagcc
     2041 gcaatccacg cagcacctcc ggaaccggac tacttcggat cttgcgtaat ccaactgagt
     2101 ggaaatgcgc cgccacggtg gcgcttgtct tcttcgttgc ttcgctcacc cttgcgcttg
     2161 ccctcacccg cgatcagaag acctcagaga tcaatgccaa aacgctcaaa gccgcagccg
     2221 caatcaaaat ctctcggagg cagccgctcc gcgtccaaaa ttgagcggtt ccgcgcgtat
     2281 cgcatataat ctcggccgac aaattgctcc acgccacaac gatccgctcc ccccgtggcc
     2341 gcgctcagcg aacactgatc tgcaagcgcc ccgccgactg ccctgtcccc agcgcaatcg
     2401 aaaactggaa ggtaccgtgt aggcagtatc cgcagcttgt ggaatgaccg cctcgttatc
     2461 atgccaccca tgagcggttc cacccc
//
LOCUS       DATMGX010000588         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_389828, whole genome shotgun sequence.
ACCESSION   DATMGX010000588 DATMGX010000000
VERSION     DATMGX010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389828"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..600)
                     /locus_tag="VNR65_09555"
     CDS             complement(316..600)
                     /locus_tag="VNR65_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18956.1"
                     /translation="MTDDDIIQLIETFDFDAGPVAVERRNRGFTLIHAETGVPIARLR
                     PIGRDDLVDILYWSLWTERWMPFGPFGRTAAPVEQAVRIIAEASIFWAGV"
     gene            729..2114
                     /locus_tag="VNR65_09560"
     CDS             729..2114
                     /locus_tag="VNR65_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ18957.1"
                     /translation="MTRHRSSSSSAAAPILPLIVISLMAPFQSFFTTPVWEHVLVLVT
                     GMVLAPGKRTVSAALRVMGLGATRDFALYHYVLSRARWNSRAIARTLLTTILDRFLPT
                     GPVVIGIDDTIERRWGRKIAARGIYRDPVRSSHGHFVKTSGLRWLSLMAMVPIPWTRR
                     RWALPFLTILAPSKRYHVARNHRHKKLTDWTRQAILQVRRWLPDRKIVIVADSSFAAL
                     DLIAAVRRHVCLVTRLRLDANLFAPAPKRRPGQRGRTAKKGRPLPKLSKLLENKTTRW
                     TKLSMPYWYGDERCILEIMTGTAIWYHSGLPPAPIRWVLVRDPTGVRDPQAFLCTDLD
                     ATPVEILGWFVSRWSIETTFQESRAHLGVETQRQWSDLAIARTTPALFGLFSLVTLWA
                     ADPTIAGSLRPRSAAWYHKREPSFSDAMAAVRRLFWSAPNLSMSRHPPDSVEIPTALM
                     ERLTEALCYAA"
BASE COUNT          532 a          763 c          723 g          540 t
ORIGIN      
        1 aatcgctgcc taaatttcgt tcaaccgtta ttgttacatt tcaggctgcg gaagcgcctt
       61 attagctgga ggccgctaga gtggccaaaa ttggacgcga acaatcaaat tcaacgacat
      121 gatagatttc ggcagcgttc ggtggctcaa tttgtcaatg atcgacgaaa ctgtacccca
      181 tattttcgcc aaaagggatc taacacgagc acgaggcgcg cgcccgatca ggcgtagacg
      241 gtcggggttg gcggagatcg tggatcagtt cgaacaaagc ctcagctcga ctttgcggat
      301 ttttgtccgc ttgtttcaga cgccggccca aaagattgat gcttccgcga tgatgcgcac
      361 ggcctgttcg acgggagcgg cagtgcgtcc aaaggggccg aacggcatcc atcgttctgt
      421 ccagagtgac caatagagga tgtcgacgag atcgtcgcgt ccgatgggac gcaggcgagc
      481 gatgggaacg ccggtttcgg catgaatcag cgtgaagccg cgattgcgcc gttcgaccgc
      541 cacgggaccg gcgtcgaaat cgaaggtttc gataagctgg atgatgtcgt catcagtcat
      601 gccgccattg tgccgtatcg gcgcgtggct gtccttgtct ggcctcgaac agggccgatt
      661 cgaaatgccg cggcctgatt gcgaagagta tcgaggacaa ttcacggcga ctcgattcta
      721 cctttgcgat gacccggcat cgctcctctt cttcatccgc cgcggcgccg atcctgccgc
      781 tgatcgtcat ttcgctgatg gcgcccttcc agagcttctt tacgaccccg gtttgggagc
      841 acgtgctggt gctcgtcacc ggcatggtgc tcgcacccgg caagcgcacg gtgagcgccg
      901 ccctgcgcgt catgggcctc ggcgcgaccc gcgatttcgc gctctaccac tatgtgctca
      961 gccgggcgcg atggaacagt cgcgccatcg cccgcacgct gctgacgacg atcctcgaca
     1021 ggttcttgcc caccgggccg gtggtcattg gaatcgacga tacgatcgag cgtcgctggg
     1081 gccgcaagat cgccgcgcgc ggcatctatc gcgacccagt gcgctcctcg cacggtcatt
     1141 tcgtcaagac gagcggcctg cgctggctct cgctcatggc catggttccc attccatgga
     1201 cgcggcggcg ctgggccttg cccttcctga ccattctggc cccctccaaa cgttaccacg
     1261 tcgcacgcaa tcaccgtcac aagaagctga cggattggac ccgccaagcc attctccagg
     1321 ttcggcgatg gttgccggat cgcaagatcg tcatcgtcgc cgactccagt ttcgcggcgc
     1381 tcgatctgat cgccgcagtt cgccgtcatg tctgtcttgt cacaaggctg cggctcgacg
     1441 cgaacctctt cgcgccagcg cctaaacgcc gtcctggaca gcgcggacgg acggccaaga
     1501 aaggacgccc gctacccaag ctctccaagc tgctcgaaaa caaaacgacg cgctggacga
     1561 agctgtcgat gccctactgg tatggcgatg aacgatgcat tctcgagatc atgaccggaa
     1621 cggcgatctg gtatcattcc ggattgccgc cggcgcctat ccgctgggtg ctcgtgcgcg
     1681 accccacggg cgtgcgcgat ccgcaagcat ttctgtgcac tgatctcgat gcgacgccgg
     1741 tcgagattct cggatggttc gtgagccgtt ggtctatcga aacgacattc caggaaagcc
     1801 gcgcgcatct cggcgtcgag acgcaacggc aatggtccga tctcgccatc gcgcggacga
     1861 cgccggcgct gttcgggttg ttttcgctcg tcacgctttg ggcggccgat cccacaatcg
     1921 caggcagcct tcgcccgaga tcggccgcgt ggtatcacaa acgcgaacct tccttcagcg
     1981 acgccatggc cgccgttcga aggctctttt ggagcgcgcc gaatttatca atgtcccggc
     2041 atccgcccga cagtgtagaa attccaaccg ccctcatgga aagactcacg gaagcgctct
     2101 gctacgcggc ataaaatccg caaagtcgag cttaggcgcg atcaatttgt tgccaatctt
     2161 ctcccggtga tccgtaacca tgaattcgta ggtgtcgtga taggcatcaa tttgaacaat
     2221 ctcaacgaag cgacagaagg aaaagaagaa ctaattataa tgtttggcac tccttatgga
     2281 gcatgctttc aatgggtcat cacgacaatt ttggatattg cagatgggta tggaagcaca
     2341 gaaaggatcg ccttcatcca cgagaccaat ggattcaaga acgaagcctt cgaatctttc
     2401 gagttcgcca aacagtaact taatcgtgga aaaatcccga tgaccctggg atttggcggc
     2461 aagcaggatt acccgccatt gcaggcggct gacgtactcg cttatgaagg cggcaaattc
     2521 ctaaagaatc tggtcccaac agaattgcct cgtcgtac
//
LOCUS       DATMGX010000589         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_6814,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000589 DATMGX010000000
VERSION     DATMGX010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6814"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VNR65_09565"
     CDS             <1..990
                     /locus_tag="VNR65_09565"
                     /inference="COORDINATES: protein motif:HMM:NF044545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18958.1"
                     /translation="ESLGPQTWLAEARGETFYAVGGTWRALAKLHMHQTGYPLTAIQG
                     YRMDARKALQVTGRYANSSPSSLKRVYGLSAGREETVPFGALLLNTIIRKMQPEDVVF
                     SAFGVREGMIYHLLSEAERTRDPLLSACEGIARLRARSDVHSKELIVWTDGLFVDPGP
                     RETKEERRLRQAACLLSDISWRAHPDYRGEQSAALIAQSGLVGVDHPGRAFLSLCAYH
                     RYERKLVGELLPKLSAMISRQQQKRALIIGQAVRLAHTLSAGMKGVLPQTSLAYEGGR
                     LVLRLPQSLAALDGEQLKRRLRSVARELGLQSDVSISSEQKPSKTTILGALLG"
     gene            1077..2027
                     /locus_tag="VNR65_09570"
     CDS             1077..2027
                     /locus_tag="VNR65_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWJ18959.1"
                     /translation="MNKDLYAILGVKRTATNDEIRRAYRKLAKDLHPDQNKNDKKAEE
                     QFKKVSAAFSVLGSPEKRKRYDAGEIDAMGNERANPFYREYAGGAQGFGKEHAGGFGG
                     AGSGGGAGDFSDIFGDLFGRRGAGGGAFTMRGMDYRYNLEISFLESVNGVKKRVQFPD
                     NETLDISVPCGVETGQTLRLRHKGGPGAGRGEPGDALIELKVQPHPLFSRDGDNITLE
                     LPISLNEAVLGARVEVPTIGGRVTVAVPKGASSGQVLRLRGKGVKNNRSGAHGDQLIT
                     LKIVLPAVIDSDLERLMQEWSSKRPYDPRARFREAAGAGG"
BASE COUNT          408 a          608 c          655 g          380 t
ORIGIN      
        1 gagagtttgg ggccccagac ctggctcgcc gaggctcgtg gcgaaacatt ttacgccgtt
       61 ggcgggacgt ggcgcgcgct ggccaagctc catatgcatc agacgggcta tcccctcacg
      121 gccatccagg gctatcgcat ggacgcgcga aaggccttgc aggtcactgg gcgctacgcg
      181 aacagctccc cctcatcctt gaagcgtgtt tacggcttat cagcagggcg agaggaaacc
      241 gtgcctttcg gtgcgcttct cctgaacacg atcatcagga agatgcagcc ggaagacgtc
      301 gtcttctcgg cgttcggagt gcgggagggt atgatttatc atctcctgag cgaggctgag
      361 cgcacccggg accccctgct gtcagcctgc gagggtatcg cgcggcttcg ggcacggtcc
      421 gatgtgcact ccaaagaatt gatcgtgtgg acagatggcc tcttcgtcga cccgggtccg
      481 cgtgaaacca aggaagagcg ccggttgcgg caggcggcgt gcctcctttc cgatatctcc
      541 tggcgcgcac accccgacta tcgaggcgag caaagcgccg cgctcattgc gcaatcgggg
      601 ctggtcggcg tggatcatcc gggccgtgca ttcctctcgc tctgcgccta tcatcgctac
      661 gagcgcaagc ttgtcggcga actcctgccc aagctttccg ccatgatttc gcgtcaacag
      721 cagaagcgcg ccttgatcat cggacaagcc gttcggctcg cgcatacact ttccgcgggc
      781 atgaaggggg ttctgccgca aacctctctt gcgtacgaag gcggaaggct ggtcctgcgt
      841 ctgccgcaaa gcctggcggc gctcgatggc gagcagctca aacgccggct tcgttcggtc
      901 gcccgcgagt tgggacttca gtcggatgtc tcgatctcat cggagcagaa accctcaaaa
      961 acaacaattt tgggcgctct cttgggataa ggggggcgta agctcattta tgacgtatct
     1021 gtaagaggag ttttccgtca taactttgcg caagcagtcc aatcgctcca ggttccatga
     1081 ataaagatct gtacgccata ctgggtgtaa aacgcacggc aacaaacgac gagatccgca
     1141 gggcctaccg aaagcttgcg aaggatctcc atcccgacca gaacaaaaac gataagaagg
     1201 ccgaggagca gttcaaaaag gtttcggcgg cgttctcggt gcttggaagc ccggaaaagc
     1261 gtaagcgcta cgacgcgggc gaaatcgacg cgatgggcaa tgagcgcgcc aacccgttct
     1321 atcgcgaata tgccggcggc gcgcagggct ttggcaagga acacgccggc ggcttcggcg
     1381 gcgcaggcag cggcggcgga gcaggcgatt tctccgatat tttcggcgat ctgttcgggc
     1441 ggcgaggggc gggcggcggc gcgttcacca tgcgcggcat ggactaccgc tacaacctcg
     1501 aaatcagctt cctggagtcg gtcaacggcg taaagaagcg cgtgcagttt ccggacaatg
     1561 agacgctcga tatttccgtt ccctgcggcg tagagaccgg gcaaacgctg cgtcttcgac
     1621 acaagggcgg cccaggcgcg ggccgcggcg agccgggcga tgcgctcatc gaactcaagg
     1681 ttcagcccca cccgcttttc tcgcgcgacg gtgataacat cacgcttgag ttgccgattt
     1741 ccttgaacga ggccgttctc ggcgcgcgcg tcgaggttcc gaccatcggc gggcgggtga
     1801 cggtcgcggt tcccaaggga gcgtccagcg gacaggtttt gcggctgcgc ggcaagggcg
     1861 tgaaaaacaa tcgctcgggc gcccatggcg atcagctcat cacgctgaag atcgtgctgc
     1921 cggctgtcat cgacagcgat ctggaacgcc tcatgcagga gtggtcgagt aagcggccct
     1981 acgatccgcg cgcacgattc cgtgaagccg caggcgccgg aggctgacgc agcttccgag
     2041 ggaaagaagg a
//
LOCUS       DATMGX010000590         5521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_109839, whole genome shotgun sequence.
ACCESSION   DATMGX010000590 DATMGX010000000
VERSION     DATMGX010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109839"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VNR65_09575"
     CDS             complement(<1..529)
                     /locus_tag="VNR65_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWJ18960.1"
                     /translation="MIPQDLSFAFTQVFWWTVPFLVVLGVVVLVHELGHFLAARALGI
                     KVETFSIGFGPEIAGFYDRSGTRWRIACIPLGGYVKFKGDESIASMPSAEVLETLTPE
                     QRRGNFHTSAIWRRVLIVLAGPVANFVLGIVIFTGMVLASGIVYQEARITCVEPDTPA
                     AKAGLEAGDRIVSVAG"
     gene            complement(649..1905)
                     /gene="dxr"
                     /locus_tag="VNR65_09580"
     CDS             complement(649..1905)
                     /gene="dxr"
                     /locus_tag="VNR65_09580"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWJ18961.1"
                     /translation="MKKNGHGSIGEPEGAGQTKRVSVLGATGTIGQHTCDLLALHPDR
                     FQTVALTANRSAKKLASLAIALKAEYAALADESMADELRALLEGTGIACGAGKSALVE
                     AGARSADLIMAAIVGSAGLAPTVAALRQGTTVALANKECLVSAGAFFMKERERCGTTL
                     IPVDSEHSAVFQSLDSLECEHVERIVLTASGGPFRTWTAEQIAAARPEDALKHPNWTM
                     GQKITIDSATMMNKGLELIEAFHLFNVGADQLSAVIHPQSIIHALVYYSDGSVIAQAS
                     LPDMRTPIAYSLAWPARLKADFKRLDLAAAGHLTFDEPDPARFPALRIAKESLRAGGA
                     APTVLNAANEVAVEAFLNRSTSFPGIAALVERTLNKAASDLSGVVPDSLEAVMYLDSR
                     AREIAKTALLEQTGACTGPSAYSVPI"
     gene            complement(1935..2768)
                     /locus_tag="VNR65_09585"
     CDS             complement(1935..2768)
                     /locus_tag="VNR65_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWJ18962.1"
                     /translation="MAKPISSELVLRINSALVLVALTLALTYAGTQTFAALILFAATL
                     MSWEWGRVVRGTGIDGIFAIQSAAIIGAGYFTLKHQSAAAVAIIFVATWLVFRLHQKL
                     DYRSDPWWSAAGVYYAGLPAIALIAIRQDPDYGFHAILYLFLVVWSADTGAFFTGKLI
                     GGPKLAPKISPNKTWSGFIGGALFACIAGVLFAAWFGHTSIPTVAALSVVLALISQGG
                     DLGESFIKRFFGVKDSSGLIPGHGGVLDRLDGLVFAAIGAGLIAAAADPLKPGRALLV
                     W"
     gene            complement(2811..3548)
                     /locus_tag="VNR65_09590"
     CDS             complement(2811..3548)
                     /locus_tag="VNR65_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HWJ18963.1"
                     /translation="MQEQAFALGKEHPYPKHIAIIMDGNGRWAAQRGLPRMEGHRRGV
                     ESTRECVRAASDLGIGYLTLFSFSSENWRRPPREVDYLMGLLRRFIRRDLAELHQNNI
                     KVRVIGSEVGVPPDLVTMFREAVALTSGNTGLQLIIAFNYGARDEIVRAAKSLAQRVE
                     SGELSAEAITQETLAEHFDTAGIPDPDLLIRTSGEQRISNFLLWQCAYSEFLFVDNFW
                     PDFTRQTLESAIASYQQRERRFGGLAG"
     gene            complement(3717..4283)
                     /gene="frr"
                     /locus_tag="VNR65_09595"
     CDS             complement(3717..4283)
                     /gene="frr"
                     /locus_tag="VNR65_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWJ18964.1"
                     /translation="MAAGSFDIKEIEKRMKGAVSALKSEFGGLRTGRASTTLLDPLMV
                     NTSYGAKMPIHQVASINAPEPRLITVQVWDKSNLHAVDKAIRESDLGLNPVVDGLTLR
                     LPIPELNQERRQELVKIARKYTEQARVAARNVRKDGMDQIKKLEKDGKISEDDSGSLH
                     TKVQDLTDRTIKEIDGLLSGKEHEIMQV"
     gene            complement(4330..5058)
                     /gene="pyrH"
                     /locus_tag="VNR65_09600"
     CDS             complement(4330..5058)
                     /gene="pyrH"
                     /locus_tag="VNR65_09600"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWJ18965.1"
                     /translation="MQPLPYTRPLIKISGEALMGSGRYGIDADTLAEIVMDIAEVVVR
                     GAGVAVVVGGGNIFRGMSGAAHGIDRVKGDFMGMLATVMNGLALEQAFKRAGQAVSLY
                     SGLPVPTICRTFSRDDAVADLEKGAVVILAGGTGNPFFTTDTTAALRAAELGCDAILK
                     ATQVDGVYSEDPKTNPRAERYPSLTFSEVLARDLKVMDAAAIALARDNAIPIVVFSLH
                     QKGAIGKVMRGEARFSVVGSDPAA"
     gene            complement(5225..>5521)
                     /gene="tsf"
                     /locus_tag="VNR65_09605"
     CDS             complement(5225..>5521)
                     /gene="tsf"
                     /locus_tag="VNR65_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HWJ18966.1"
                     /translation="ERERAIFTEQAKESGKPEKVIKQMIEGRIKKFYQEVILLKQAFV
                     VNPDLTVEKALKEAETAVGAPIVVTAFVRYELGEGIKADKGDFAAEVAAAAGKQ"
BASE COUNT         1169 a         1662 c         1540 g         1150 t
ORIGIN      
        1 tgccagccac ggacacaatc ctgtctcctg cctcaagccc agccttcgca gcaggagtat
       61 caggctctac acaggtgatt cgcgcttcct gatagacgat gccgctcgcg aggaccattc
      121 cggtgaagat gacgataccc aaaacgaagt tcgcaaccgg gcccgccagc acaatgagaa
      181 cgcgccgcca aatggccgag gtgtggaaat tgccccttcg ctgctctgga gttagcgtct
      241 caagaacctc ggcggatggc atgctggcaa tgctttcgtc gcccttgaat tttacataac
      301 cgccaagggg aatgcaggcg atccgccatc gcgtgccgct gcggtcgtaa aaccccgcta
      361 tctccggccc gaagcctatc gaaaacgttt caaccttgat cccgagcgcc cgtgctgcca
      421 agaaatgccc gagctcgtgg acgaggacga ccacgcccag cacgaccaga aatggcacag
      481 tccaccagaa gacctgggtg aatgcgaatg acaaatcctg aggaatcata gggtaacctt
      541 ccgggcaggc aggcgcttag cctgttgcgg cgcgccagcc tctcatcgtg ggagcatgaa
      601 acggaaatac gctcgaagaa gctccagata ggaagcttct tcgtgtactt aaataggaac
      661 tgaataagcc gaaggcccag tacaagcgcc ggtctgttcg agaagagcag tttttgcgat
      721 ctcgcgagcg cggctatcga gatacatgac cgcttccagg ctgtcgggga ccacccccga
      781 aagatcggat gccgccttgt tcaaagtgcg ctccaccagc gctgcaatgc cggggaacga
      841 tgtcgacctg ttgagaaacg cttcaaccgc gacttcattt gccgcattga ggacagtggg
      901 cgcggctccc cctgcccgca gcgactcttt tgcgattcgc agagcgggga agcgagccgg
      961 gtccggttca tcgaaggtca agtggcctgc ggcggcgagg tccagcctct tgaaatcggc
     1021 cttaaggcgc gccggccacg ccaggctgta agctatcgga gtgcgcatgt cgggaaggct
     1081 ggcttgagcg atgacggaac catcactgta atagacgagc gcgtgaatga tggactgcgg
     1141 atggattacc gccgaaagtt gatcggctcc cacattgaaa aggtgaaagg cttcgatcag
     1201 ttcgaggccc ttgttcatca tggtggcgct gtcgatggtg attttctggc ccatcgtcca
     1261 attcggatgc ttgagagcgt cttcggggcg tgccgccgcg atttgctcag ctgtccaggt
     1321 gcggaaaggc ccgccggaag cggtgaggac gatcctctcg acatgttcgc actcgaggct
     1381 gtctagcgac tggaacaccg ccgaatgctc agaatcgacg gggatcagtg tcgtgccgca
     1441 gcgttccctt tccttcatga aaaaggcgcc ggcggaaaca aggcattctt tgtttgcaag
     1501 cgcgaccgtc gttccctggc gaagggctgc cactgtgggc gcgaggccag cggagccaac
     1561 aatcgcggcc atgataaggt cggcggaccg ggccccagcc tccaccagcg ctgattttcc
     1621 ggctccgcac gcgatgccgg ttccctcaag gagcgcgcga agctcgtcgg ccatgctttc
     1681 gtcggcgagc gccgcatatt cggccttcaa agcaatcgcg aggctcgcca gctttttggc
     1741 gctccggttt gccgttaacg ccacagtctg aaaacggtcc gggtgaaggg caaggaggtc
     1801 gcaggtgtgc tgcccgatcg tgcccgtcgc gccaagcacg ctcacgcgtt tcgtttggcc
     1861 cgcaccctcc ggctccccga tggagccatg cccgtttttc ttcatctttg ctccatttgc
     1921 cagcgactct atgctcacca aaccaagagc gcgcgtcccg gctttagcgg atccgccgcc
     1981 gctgcgatga ggccggctcc gatcgctgca aaaacaagcc catcaagacg gtccagcaca
     2041 ccgccatgtc cgggaataag accgctcgaa tccttcaccc caaaaaagcg cttgatgaaa
     2101 gattccccaa gatcgccgcc ctgggagatg agtgccagca caacgctgag agcggccact
     2161 gtagggatgg acgtatgccc gaaccatgcg gcaaaaagca cgcccgctat gcaagcgaaa
     2221 agcgccccgc cgataaaacc agaccacgtt ttatttggcg agattttcgg ggcaagctta
     2281 gggccgccaa tcagttttcc ggtaaaaaag gcacccgtgt ccgccgacca tacgacgaga
     2341 aacaggtaga gaatggcgtg aaatccgtag tcggggtcct gccggatcgc gatcaacgcg
     2401 attgcaggga gaccggcgta gtagacgccg gcagccgacc accagggatc actccggtag
     2461 tctagctttt gatggaggcg aaataccagc catgtcgcga cgaaaatgat ggcgacagcg
     2521 gccgcagact gatgcttcaa agtgaaataa cccgcgccaa taattgccgc ggattgaatt
     2581 gcgaaaatcc cgtcgatccc ggtgccgcgg acgacgcgtc cccattccca gctcatcagg
     2641 gttgcggcga acaggatcag cgccgcaaag gtttgcgtcc ccgcataggt cagtgcaagc
     2701 gtcagcgcca ccagaacgag cgctgagttg atcctgagaa ccagctctga actgataggc
     2761 tttgccatta tccttcgccg ttaaagggcg ggccgtagat aacgcgggcg ctaccctgca
     2821 agcccgccga aacggcgttc acgctgctgg taggatgcga tcgcgctttc caatgtctgt
     2881 cttgtaaagt caggccagaa attatcaacg aataaaaact cagagtaggc acactgccac
     2941 aagagaaagt tgctgatacg ctgctcgccg ctcgttcgaa tgagcaggtc ggggtcaggt
     3001 atgcccgctg tgtcgaagtg ctccgccaac gtttcctggg ttatggcctc ggcagagagc
     3061 tctcctgatt ctacacgctg cgccaaagac ttagcagcac gcacgatctc atcacgagcg
     3121 ccataattga aagctataat gagctggagg ccggtgttcc cactcgtcag cgcgaccgcc
     3181 tcgcggaaca tggtgacgag gtccgggggc acgcccacct ctgagccaat aacacgcacc
     3241 ttgatgttgt tctggtggag ttcggcgagg tcgcggcgaa tgaaacggcg caaaagcccc
     3301 atcaggtagt cgacctcacg gggaggccgt ctccaattct ccgacgaaaa gctgaaaagc
     3361 gtgagatagc caatccccag atccgaagca gcccgcacac attcccgcgt gctttcgacg
     3421 ccgcgacgat ggccttccat gcgtggtaac ccacgctgcg ccgcccagcg gccgttgccg
     3481 tccataatta tagcaatatg ttttgggtaa ggatgctctt tcccgagcgc aaacgcttgt
     3541 tcctgcaatc tcactcttgg ccacccatta ttccctcgcg gtccagccgc tgtctctaac
     3601 ccatatcaat gagacaataa atcaaaccta cttattggct gacggttttc cgcagttgca
     3661 gccagctgcg caaatttttt aagagcctga gcctgtgtga gcttgcctta agcactctac
     3721 acttgcatga tctcgtgctc tttcccagac agaagtccat cgatctcctt aatggtacgg
     3781 tcggtgagat cctgaacctt ggtatgcagc gagccgctgt cgtcttcgct gatttttccg
     3841 tctttttcga gcttcttgat ctgatccatg ccgtccttgc ggacgttcct tgcggcgacg
     3901 cgagcctgct ccgtgtactt tctcgcaatt ttcacgagtt cctggcgccg ttcctggttg
     3961 agttccggga tcggcaagcg aagcgtcagc ccatccacca ccggattaag gcccaagtcc
     4021 gattctcgga tggccttgtc caccgcgtga agattgctct tgtcccaaac ctggacagtg
     4081 atcaggcgag gctcgggtgc gttgatcgaa gccacctgat ggattggcat ttttgcgccg
     4141 taggaggtat tcaccatcaa aggatcgagc aaggtcgtgc tcgcgcggcc cgttctgagg
     4201 ccaccgaact ccgacttgag cgcgctcacg gcgcctttca tgcgtttctc aatttccttg
     4261 atatcgaaac tgccggctgc catttccgat ccccttttat tcttgaggcc acgtattcct
     4321 taccttcatt tatgccgccg gatcgcttcc cacaacgctg aagcgggctt ctccgcgcat
     4381 aacttttcca atagcgcctt tctgatgcag agagaacacc acgattggta ttgcattgtc
     4441 gcgggcaagt gcaatagcgg cggcgtccat cactttcaga tcccgggcca gcacctctga
     4501 gaaggtgagg ctggggtagc gttcggcacg cgggttcgtc ttcggatcct cagagtagac
     4561 gccgtccact tgggtggctt tgaggatcgc atcgcagcca agttcggcgg ctctgagcgc
     4621 cgccgtcgtg tctgtggtga agaatggatt tccagtaccc ccggcaagaa tgacaactgc
     4681 gcctttctcg agatcggcga ccgcgtcgtc acgcgagaat gtcctgcaaa tcgtagggac
     4741 gggcaggccc gaatagagcg aaacggcctg tcctgcgcgc ttgaaggcct gttccagggc
     4801 gagaccgttc atcactgtcg ccagcatgcc catgaagtca ccctttacac ggtcgatgcc
     4861 atgggccgcg cctgacatgc cccgaaagat attcccgccg ccgacgacaa ccgcaacgcc
     4921 ggcgccgcgc acgacaacct ccgcgatgtc catgacgatt tcggcgagcg tgtccgcgtc
     4981 gatgccataa cggccacttc ccataagcgc ctcacccgag attttgatca gcgggcgggt
     5041 atagggtaat ggctgcatca tcttttcctt tggcgagcgc acggctacgg caccttgccg
     5101 tcgctttgat aggcgtggcc gctcgatcaa cccgccttta atcggaatcg attaaaggcg
     5161 aaaaggtgat cgattctgaa gtgctagagc agcttatccg catccaaacg gacgcgggcc
     5221 cgctctattg tttgccagcg gccgccgcaa cctctgctgc gaagtctccc ttatcggcct
     5281 taatgccttc accaagctcg tagcggacaa aagccgtgac cacgatcgga gcgccaaccg
     5341 cagtctcagc ttccttgagt gccttctcaa ccgtcaggtc gggattgacc acgaaagcct
     5401 gcttcaggag aatgacttcc tggtagaact tcttaatccg gccctcgatc atctgtttga
     5461 tgaccttctc aggtttgccg ctttccttcg cctgctcggt aaaaatggca cgctcgcgtt
     5521 c
//
LOCUS       DATMGX010000591         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_35898, whole genome shotgun sequence.
ACCESSION   DATMGX010000591 DATMGX010000000
VERSION     DATMGX010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35898"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VNR65_09610"
     CDS             complement(<1..459)
                     /locus_tag="VNR65_09610"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18967.1"
                     /translation="MNNIAASSCETKAAQIGPTPLTPDELRRMDAYWRACNYLCAGML
                     YLRENPLLREPLKPEHIKNRLLGHWGSDPGQTFTWVHLNRLIKKYDLDLIYISGPGHG
                     APATLANSYLEGVYSEIYPEMGEDASGMQKFFKQFSFPGGIGSHCTPETPG"
     gene            925..1125
                     /locus_tag="VNR65_09615"
     CDS             925..1125
                     /locus_tag="VNR65_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18968.1"
                     /translation="MPQSPHDRLAELHNLAAHAHAAAATAHGKGDHLTAHELSEQAHQ
                     HSMNAYKHAKKLMTVATQSKKP"
     gene            1174..2418
                     /locus_tag="VNR65_09620"
     CDS             1174..2418
                     /locus_tag="VNR65_09620"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HWJ18969.1"
                     /translation="MNVFVLNSGSSSLKFQIIATDLDRIKQHQDERVCRGEVERIGGE
                     AIVTFQKGADAKQKFTAPLRDISAALEYLIRYIASDKSGVAEIKSSGDMHAVGHRVVH
                     GGEMFSESALIDAKVLQDIEDCIDLAPLHNPNNIKGIVAARELFGKDVPQVAVFDTSF
                     HHSIPEQAFLYALPYHLYRRHRIRRYGFHGTSHRYVAFRYRALRELTRDQTNIITLHL
                     GNGCSATAIRRGNSVDTSMGMTPLEGLVMGTRSGDLDPAIVNLVATKEGMSPSEVDTL
                     LNTQSGLLGISGLSNDMRMLQAESAENHARRVHLAIEVFCYRARKYIGAFLATMGGAD
                     AVIFTGGIGENSPEIRARICEGMDWAGLKIDTAKNQQTVGREGLISTDGSRLLAYAIP
                     TDEELLIARDTVRVILGEPHPS"
BASE COUNT          568 a          750 c          704 g          539 t
ORIGIN      
        1 accaggcgtc tcgggtgtgc agtggcttcc gattccgccg ggaaacgaga attgcttgaa
       61 gaacttctgc ataccggaag catcctcgcc catctccgga taaatctccg aatacactcc
      121 ctcgaggtag ctgtttgcga gcgttgctgg agctccatgg cccggccctg aaatatagat
      181 gaggtcgagg tcgtacttct tgatgagccg gttcagatgc acccaggtga aggtctgtcc
      241 aggatcggaa ccccagtgtc cgagcagcct gttcttgatg tgctcgggct tcaagggctc
      301 gcgcagcagc ggattctcgc gcaggtacag catgcctgcg caaagatagt tacaggcacg
      361 ccagtaggcg tccatcctcc gcagttcatc gggagtcaat ggcgtaggtc cgatctgagc
      421 ggccttggtc tcacaggaag aagcggcgat gttattcaag tggttacctc gtgcaatgga
      481 gtgggttggg ttctcgatgg cctacattcc ttgttggatt gacccgcacg gtacacttca
      541 tgccaatcat gagatgttcc tgtgcaggaa cggcgaagac agccgtctcc cactcaaatg
      601 agccgattcg gcggacgccg cgtccgtgat ctcatcatgg atgtcacgct tcgccctccg
      661 gatgcacgac acgatatcca accgcctgca tcgcaggcat ccgcgggtca cattacgctt
      721 cgagaaacat cctgacgcgg agcctcgtcg attactgtgc cgaatcgcac agcgcgtcat
      781 cttctagggt gggacacacc gagtgcagcg ttatcacagc acgcgcgaca ttctgttttg
      841 ttcatccctc acctgcaata ataggcgaat ggagtgacaa tccgcgctcc cacttcaata
      901 cattcgattc aacaggagaa ttccatgcca cagagtccgc acgatcgact cgcagaactt
      961 cacaacctgg cagctcacgc ccatgctgcc gccgccaccg cccatggcaa gggagatcat
     1021 ctgaccgctc acgagctgag cgaacaggcg caccagcact ccatgaacgc gtacaaacac
     1081 gccaaaaagc tcatgacggt tgccacccag tccaaaaaac cataggaccc cgggacttgc
     1141 tggtacgata catcccaaaa gagaaagccc ggcatgaacg tctttgtact caattccgga
     1201 agttccagcc tgaagtttca gatcatcgca accgatctgg atcgtatcaa gcagcatcag
     1261 gatgaacgcg tctgccgcgg cgaagtagaa cgcatcggtg gggaagcgat tgtcacattc
     1321 cagaagggcg ccgacgcaaa acagaagttc accgctcctc tgcgagacat ttcggcggcg
     1381 ctggaatact tgattcgtta catcgcctcc gacaaatcgg gagtcgctga gatcaagagt
     1441 tcgggtgaca tgcacgcggt tgggcatcgc gttgtacacg gcggcgagat gttctcggag
     1501 tcggccctta tcgacgccaa ggtattacag gatattgaag actgcataga ccttgcgcca
     1561 cttcacaatc cgaacaacat caagggcatc gtggctgcgc gagaactctt cggcaaggat
     1621 gttccccaag tggccgtatt tgatacttcg tttcatcatt cgatccccga gcaagctttc
     1681 ctgtacgccc tcccatatca cctgtatcgg cgtcaccgca tccgccgata cggcttccat
     1741 ggaacctcgc atcgttacgt tgcgtttcgc taccgcgcgt tgcgcgaact gacgcgggat
     1801 cagaccaaca tcatcaccct tcatctcggg aacggctgct cagcaaccgc aattcgccga
     1861 ggaaattcgg tggatacctc catgggcatg accccgctcg agggtctggt gatgggcacg
     1921 cgttcgggag atctcgaccc tgcgatcgtg aatctcgtag cgacaaaaga agggatgtcg
     1981 ccgtcagagg tcgatacgct tctgaatacg caatcgggtt tgctgggtat ttccggactg
     2041 tccaacgaca tgcggatgtt gcaagccgag tcggcggaaa accacgctcg tcgcgtacat
     2101 ctcgcgattg aagtcttttg ctatcgcgca cggaagtaca tcggcgcatt cctcgccacc
     2161 atgggtggtg ctgacgcggt gatcttcact ggcggaatcg gagaaaactc gccggagatc
     2221 cgcgcccgta tttgcgaagg catggattgg gccggtctta agatcgacac cgcgaaaaac
     2281 cagcagacgg ttggccgcga aggcctcatc agcacggacg gttccaggct gctcgcctat
     2341 gccatcccca cggatgaaga attgctcatc gctcgcgata ccgtccgggt catactcggt
     2401 gagccgcacc cctcgtgatg cggtttgacc acgcaggggg ccgtcgcggg ctctcttgca
     2461 tgggttcatc ggcaggtgca cttactccga ttgtcgcgca atacattcct gagcaacgct
     2521 ctcatgggat aatgcggcag ttactggagg gccccggatg g
//
LOCUS       DATMGX010000592         4665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_261684, whole genome shotgun sequence.
ACCESSION   DATMGX010000592 DATMGX010000000
VERSION     DATMGX010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261684"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1373
                     /locus_tag="VNR65_09625"
     CDS             312..1373
                     /locus_tag="VNR65_09625"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HWJ18970.1"
                     /translation="MARWHDSHLRHYLCVAAALPAVLNAGTIMAFECGQAKSPAEKAI
                     CSDPAAKASDEAMGGAFTDLKSLLDPDQAKALLVNQRDWLAQRNEYCTTGSDAPACIK
                     EMNDARVLLLGGKPKSDPGSSVRLIPFFVDTPGTKTAPEIHVIMYKHHGDSGPLAKTL
                     DAAIGTILDSAKKKENFLADKEVTFERKAKVFYASDRLISIEIDGFDYVQGGAHGAVT
                     TQMLNIDPQSGRELKITDLLDGAGRTKVINLCIDQLLREKTDRFAQAAKETAESEHVQ
                     ADPVEPLSADDVKTIRKYAKPVIETLSSWRFDAEGAAILFDQDEIGAHAEGAFECNFP
                     YAQLRTLAKASFPLPPHRS"
     gene            complement(1601..1879)
                     /locus_tag="VNR65_09630"
     CDS             complement(1601..1879)
                     /locus_tag="VNR65_09630"
                     /inference="COORDINATES: protein motif:HMM:NF018350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HWJ18971.1"
                     /translation="MTIALRPRPKHVIGPDGSPLTMADLPAPGTKRWVIRRKAEVIAA
                     VRGGLLSLEEACSRYMLTVDEFLSWQFSIDQHGLAGLRTTRIQQYRQQ"
     gene            complement(2068..2265)
                     /locus_tag="VNR65_09635"
     CDS             complement(2068..2265)
                     /locus_tag="VNR65_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18972.1"
                     /translation="MSNVIQFRCRDTAQDEAPDIDLLTAVDVAIRDLQDILQGCGASV
                     CEQVMQCRQMLERAFNAAVQS"
     gene            2491..2688
                     /locus_tag="VNR65_09640"
     CDS             2491..2688
                     /locus_tag="VNR65_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18973.1"
                     /translation="MAKKKRTIRAWTNQEFRDLKRFAKDKVPVATISKALKRTAGALR
                     QKAGALGITIGHQRRKKKRTK"
     gene            complement(2731..2859)
                     /locus_tag="VNR65_09645"
     CDS             complement(2731..2859)
                     /locus_tag="VNR65_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18974.1"
                     /translation="MHIANLVLPVCAIIRSASARASGGWSGKSHVWVTLVVELRRA"
     gene            complement(2902..3285)
                     /locus_tag="VNR65_09650"
     CDS             complement(2902..3285)
                     /locus_tag="VNR65_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HWJ18975.1"
                     /translation="MRSVIFAAMLTVVSISGAQAQDAAADEKIFGVCKACHQIGETAK
                     NLFGPVLNGVIGRPAGTYPGYSYSAANKNSGITWDEATFREYIKDPRAKIPGTKMIYA
                     GLRDEQKVNDLLAYLKQFGPDGKKK"
     gene            complement(3385..3657)
                     /locus_tag="VNR65_09655"
     CDS             complement(3385..3657)
                     /locus_tag="VNR65_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18976.1"
                     /translation="MIAPEANSAFVANMEDVLEVYQRPRDPERRVVCLDEASKQLIVE
                     TRAAVPAKPGRKARHDYEYERNGVANLFMLFAPLEGWRRVKVTDRS"
     gene            complement(3654..4148)
                     /locus_tag="VNR65_09660"
     CDS             complement(3654..4148)
                     /locus_tag="VNR65_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ18977.1"
                     /translation="MAAKEVSVKKYVVRLSAKERARLDDLVHAGRRPAQLLTKARILL
                     KADVSDAGDGWSDSRIAEALDTSIANIERTRRQLVEEGFESVLTRKYNPNSARPRIFD
                     GAAEAKLIALTCGPAPAGHAKWSLRLLEEKVVELNIVQRASDNTIGPTLKKTFSSRIS
                     SSNG"
     gene            complement(4269..>4665)
                     /locus_tag="VNR65_09665"
     CDS             complement(4269..>4665)
                     /locus_tag="VNR65_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18978.1"
                     /translation="FAEKSGQPEPALHVYVSDPPTVLDEDLAIIQALGTAQQTVGGES
                     PAAIIRRPGADAVHLTAYGVPCVAFGPGGRTHPDTNGASMHAFGEHRPIKTEVHKRLN
                     TWLKRRLVGLHDRAISPAANEHDDSCGLR"
BASE COUNT          961 a         1380 c         1341 g          983 t
ORIGIN      
        1 cccgccgtgt cttgggggca gaggatcatc actataccca ttgagcggct cctcctcttg
       61 cgtttgggcc gagatacggg ccggcgaact gctggtggag atgaaggaga agggcgaaag
      121 agccaaacaa gggcagcccg agttgacggt agccgtccga acctcgcttc tttcttgcag
      181 gaaggccgga aaaacgcgag tattcacgcc aatatgggag ccatctgggg aatcccggtt
      241 tctatcttga gcagcccgaa tgatcgcgcc cgactttcct gctcggcaca gcgcgcgtgg
      301 acacgactga aatggcccgt tggcacgact ctcatctccg ccactatctc tgtgtcgccg
      361 cggcgctccc tgcagtcctg aacgctggaa cgatcatggc cttcgagtgc gggcaggcca
      421 agagcccggc ggagaaagcg atctgctccg atccggcagc caaggcatcg gatgaggcga
      481 tggggggagc cttcaccgac ctgaaatcct tgctcgaccc ggaccaggca aaagcgcttc
      541 tggtaaacca gcgcgattgg ctggctcagc gtaatgaata ttgcacaacg ggctccgatg
      601 cccccgcttg tatcaaagag atgaacgatg ctcgtgtcct gcttctcggg ggcaagccca
      661 agtcggatcc aggttcgtcg gtgcgactca ttcccttctt tgtagacacg cccggaacca
      721 agaccgcgcc cgagattcac gtcatcatgt acaagcatca tggcgattcg gggccgctcg
      781 ccaagacgct ggatgctgcg atcggaacaa ttctggattc tgctaagaaa aaggaaaact
      841 tcctagccga taaagaggtc acttttgagc gaaaagcaaa agtcttctac gcatccgatc
      901 gtctgatttc gattgagatc gatggcttcg attatgtgca gggcggcgcc cacggcgctg
      961 tcacaaccca aatgctcaat attgatcccc aatccggccg cgagctgaaa ataacggatc
     1021 tcctggatgg cgccggccgg acaaaggtca ttaatctctg catagatcag cttctgcgtg
     1081 agaaaacgga tcgatttgct caggctgcaa aagagacagc cgagtcggaa catgttcagg
     1141 ctgatcccgt cgagcccctc tcggcggacg acgtgaagac tattcgcaag tatgccaaac
     1201 ctgtgatcga aactctgtcg tcatggcggt ttgacgctga gggcgccgcc atccttttcg
     1261 atcaggatga aatcggcgca cacgccgaag gggctttcga gtgtaacttt ccatacgcgc
     1321 agctccgcac gctggcgaag gctagttttc ctctgccccc acacaggtcg tgagcctgcc
     1381 agttacggac attagccgca cagaactccg ccgtcgtcat cgtagcgacc gacaggtgct
     1441 cgcggtcggc ctcgaccatg accccacttg tttcctgaag accgcttgtt gcttagagcc
     1501 ttgcggcgga attggctaag cgagtgaaca gcttgcggac aagcgatgct gacgcgacgg
     1561 tggtcgaacg ggggtctgat gaagatgcgg gattaagccg tcattgctgc cggtattgct
     1621 ggatgcgcgt agtgcgtagc ccagcgaggc cgtgctggtc gatcgagaat tgccacgaaa
     1681 gaaattcgtc gaccgtaagc atataacggc tgcaggcttc ctcaagggag agcaagccac
     1741 cacgcacggc cgcaatcacc tcggccttgc gccggatgac ccagcgtttc gtacccggcg
     1801 ccggcaaatc cgccatcgtc aatgggctgc cgtccgggcc gatcacgtgc ttgggcctcg
     1861 gtcgaagggc gattgtcatg aaaactctca cgcagataac tgaacaactc agggaatgct
     1921 acctgcattc ggctaaaaaa cacttacgta catcggacca aatttaagtt tggaaatgca
     1981 gacgtgctga caccgtgctg acaccgtgct gacaccgtgg tggccttggc ggctcggatt
     2041 gaatcccggc tttgccccgg cggccgacta agactgaacc gcggcgttaa atgctcgttc
     2101 aagcatttgc cgacactgca taacctgctc gcaaaccgaa gcgccgcagc cttgcaaaat
     2161 gtcttgaaga tcgcgaatgg cgacgtccac ggcggtcaga agatcgatat cgggcgcctc
     2221 atcttgcgcc gtatccctgc atcgaaactg tatgacgtta ctcatgggta ctcttgccgg
     2281 cgaatcacac tggtttgcga tcgtcattag gtcggtaaaa ggttaatcgc tccgttacgg
     2341 ccggcttccc tcgaaacgcc tatgcgagac ggacctgccg tggcagagag ggggactgca
     2401 acgacatcca ccggttagga taaaattgat ggatgtcatt gtagagcgtg catcgtgaca
     2461 cttactgttc aaccctcaat gaggacgata atggccaaga agaagcgcac catccgtgcc
     2521 tggacgaacc aggaatttcg cgacctgaag agatttgcga aggacaaagt gccggttgcc
     2581 accatttcaa aagcgctgaa gcggacggct ggagcgctac gtcagaaggc gggcgcgctg
     2641 ggcatcacga ttggccatca gcgccgcaag aagaaacgaa ccaagtaggg tccgcgcggc
     2701 ctattcctcg taccaccacg ttcgttcagc tcaagcgcgg cgtagctcga caacaagggt
     2761 gacccagaca tggcttttcc ccgaccagcc tccgctcgca cgcgcgcttg cggagcggat
     2821 gatggcgcag accggcagga cgagatttgc gatgtgcatc ccgtatctct aagccggaga
     2881 tgatgctaag gcgccgtggt attacttctt ctttccatcc ggcccgaact gcttaaggta
     2941 cgccagcaga tcgttcacct tctgctcatc cctcaatccg gcgtagatca ttttggtgcc
     3001 gggaattttg gccctcggat ccttgatata ttcgcggaaa gtcgcctcgt cccaggtgat
     3061 gcccgagttc ttgttcgcgg ccgaatagga gtagcccggg taggtccctg cggggcggcc
     3121 gatcacgccg ttcagcaccg gtccgaacaa attttttgcg gtctcgccga tctgatggca
     3181 cgccttgcac acgccaaaaa tcttttcgtc ggccgcagcg tcctgcgcct gggccccgct
     3241 tatgctgacc accgtcagca ttgcagcaaa tatcacactt ctcatctcat tttccttctt
     3301 ccggtttagc ctccctcacg gtcggcacct ttcgtctttg gttgcggttc acaatacggt
     3361 cgagattccg ggctccacgg caaatcaaga acggtcggtg acttttacgc gccgccagcc
     3421 ttcaagcggg gcgaacagca tgaagagatt cgcgacgcca ttgcgctcgt actcgtaatc
     3481 gtgacgcgcc ttgcgtccag gcttggcggg aacagccgcg cgcgtctcga cgatcaattg
     3541 tttcgaggct tcgtcaaggc acacgacgcg gcgctcggga tcacgcggtc tctgataaac
     3601 ttccagcacg tcttccatgt tggcgacaaa ggcggagttg gcctccggcg cgatcaccca
     3661 ttgctgcttg agatgcggct tgagaatgtt tttttgagtg tcggcccaat cgtattgtcg
     3721 ctggcgcgct gaacgatgtt gagttcgacg actttctcct caaggaggcg caggctccat
     3781 ttggcgtgcc ccgccggggc cggcccacat gttagcgcga tcagttttgc ctcggccgcg
     3841 ccgtcgaata tccgcggccg ggcggagttt gggttgtatt tgcgggtcag cacggactcg
     3901 aacccctcct cgaccagttg gcgtctcgtc cgctcgatat tggcgatgct ggtgtccagt
     3961 gcctcggcga tccgactgtc gctccaccca tcgcccgcgt ccgacacgtc ggcctttaat
     4021 agaatgcggg ccttggtcaa caactgcgcg ggacgcctgc ccgcgtggac caagtcgtca
     4081 agtcgcgcgc gctctttcgc gctcaaccgg acaacatatt ttttgactga aacttctttc
     4141 gcggccacga atcctgcagg cgcagctgca tccggaggca ttggaggcag cagcggcgga
     4201 atctggacag aagcttaccc tgcgccgcca tcccggatac gatcactcct actggtttat
     4261 ccaaagcttc atcgaagacc acatgaatca tcatgctcgt ttgctgcagg gctgatagcg
     4321 cgatcgtgta atcccacgag gcgccgcttc agccaagtgt tcaagcgctt gtgtacttct
     4381 gttttgatcg gcctgtgctc gccgaacgcg tgcatggagg caccgtttgt atccggatgc
     4441 gtgcggccgc ccggcccgaa cgcgacacac ggcacgccgt aggcggtcaa atgcacggcg
     4501 tcggcgccgg ggcggcgaat gatcgccgcc gggctctcgc cgccgactgt ctgctgcgct
     4561 gttccgagcg cctggatgat tgccagatcc tcgtcgagca cggtcggcgg atcgctgaca
     4621 tagacatgaa gtgcgggctc tggctgcccg ctcttctcgg cgaaa
//
LOCUS       DATMGX010000593         4702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_177201, whole genome shotgun sequence.
ACCESSION   DATMGX010000593 DATMGX010000000
VERSION     DATMGX010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177201"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VNR65_09670"
     CDS             complement(<1..535)
                     /locus_tag="VNR65_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HWJ18979.1"
                     /translation="MQEMKLQDLKVKSPTELLSFAEEFAVENASALRKQELMFAILKQ
                     LAAREIEITGNGVVEVLQDGFGFLRSPDSNYLPGPDDIYVSPSQIRRFGLRTGDTVEG
                     QIRGPKDGERYFALLKVNKINFDDPEKIRHKIHFDNLTPLYPNDWLKMETDRPESKDL
                     SPRVIDIVAPLGKGQRGL"
     gene            complement(789..1025)
                     /locus_tag="VNR65_09675"
     CDS             complement(789..1025)
                     /locus_tag="VNR65_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWJ18980.1"
                     /translation="MNSLTNFMSNLLLYSKAVHLIAAFDGVAGLFYLPRLFAYHAQTG
                     VVTTQAETFKAMERQLLNGIMNPAIVFFVIVQPF"
     gene            complement(1050..2108)
                     /gene="hemE"
                     /locus_tag="VNR65_09680"
     CDS             complement(1050..2108)
                     /gene="hemE"
                     /locus_tag="VNR65_09680"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWJ18981.1"
                     /translation="MKTPSEAAVKQTTPKFLEALAGKAHKAPPMWLMRQAGRYLAEYR
                     AVRAQAGSFLNLCYNPMLAAEVTIQPIHRFGFDAAIIFSDILVVPHALGQHLEFREGE
                     GPHLEPIKSLSDVSKLSVSKAQSTFEIVYEAIHKAIVGLEGRAPLIGFCGAPWTVASY
                     MVEGRGSTDYKAAKLLAYREPKTFGAILDLLVEASSAYLIGQANAGARALQIFDSWAG
                     ALADNDFEAFVIEPTARIVKQVKAAHPHVPIIGFPRGSSPQYEEYVAKTGVDALGCDT
                     AAPLKVMAKMQAHLTVQGNLDPLLLLAGGDAMDRRVKAILDSLASKPYVFNLGHGILP
                     ETPIENVERLIRLVRAEA"
     gene            2431..3318
                     /locus_tag="VNR65_09685"
     CDS             2431..3318
                     /locus_tag="VNR65_09685"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HWJ18982.1"
                     /translation="MADDGAKPGLGGKEGLSSPESTPIPTPKHFHLHLVSDASGETLT
                     TMAKAAVVQLPGAKPNRHMHPLVRNPKQLATVLQHIESAPGIVLYTLMNPDLAELLEK
                     RCAELEVPCIFVLKPVLQAFEQYLKMPARAVVAGQHTLDADYFRRIEAMNFTLIHDDS
                     QSTEDLEKADIVLVGASRTSKTPTSIYLANRGFKTANVPLVPNIPLPPTLKELKTPFV
                     VGLVASPERLAEIRRNRLLALNSNKFDDYIDADAIKDEIAFTKRLCAQHGWQVLDVTR
                     KSIEETAAAIIALYEGSRR"
     gene            3372..3974
                     /locus_tag="VNR65_09690"
     CDS             3372..3974
                     /locus_tag="VNR65_09690"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWJ18983.1"
                     /translation="MQSDYPEIILASASSARGAILAHAGVRFHQRPSMVDEAEEQKHF
                     AGNTTPTEVALALARLKADSVLEMDPGAIVIGADQVLAIGNELLQKPGSREEARDQLL
                     KLRGRTHYLHSGAVLLYKDHAATFSDIATMTVRDFSDEFLDWYLETAGEGVLTSVGAY
                     HIEGLGIHLFSEVKGDYFTILGLPILPVLAELRRLSILMK"
     gene            3986..>4702
                     /locus_tag="VNR65_09695"
     CDS             3986..>4702
                     /locus_tag="VNR65_09695"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWJ18984.1"
                     /translation="MTRACVIGWPISHTLSPAIHRYWLKAYGLDGEYGKAAVEPKEFE
                     GFLMNLAANGFSGGNITVPHKIEAHRFCERRDAAAEAIGAVNTVWLERGRLAGSNTDA
                     FGFLANLDSEASGWDRGGPAVVIGAGGAARAIIWALRQRGFEDIRIVNRTKPRAEELA
                     APFPPASAHGLEELGPALEGANFIVNTSTLGMNGAPPLNVDLSAAAREATVCDIVYSP
                     LATDLLNEARRRGLRAVDGLG"
BASE COUNT         1014 a         1314 c         1359 g         1015 t
ORIGIN      
        1 tcagaccgcg ctggcctttg ccgagaggcg ccacgatgtc aatgacgcgc gggctcaaat
       61 ccttcgactc cggccggtct gtctccatct tcagccagtc gttaggataa agcggcgtta
      121 gattgtcgaa gtggatcttg tggcggatct tctccggatc gtcgaagttg atcttgttga
      181 ccttcaagag cgcgaaatac cgctcgccgt ctttggggcc gcggatttgc ccctccaccg
      241 tgtcgccggt cctgagacca aagcgccgga tctggctcgg gcttacatag atatcgtccg
      301 gtcccggaag atagtttgaa tcgggggaac gcaggaagcc aaagccgtcc tggagcacct
      361 cgacaacacc atttccagta atttcgatct cccgcgcggc gagctgcttc aggatagcga
      421 acatcaactc ctgctttcgc agggcgctcg cattctcgac tgcaaactct tcggcgaagc
      481 taagaagctc cgttggagac ttcactttaa gatcttgaag tttcatttct tgcatgatgg
      541 gggcgatttc cgggaggtga aaaatctggt gcagggaaaa cctgcgggcc ggatgaaatg
      601 aggactgtcg ctaggccgct tgtctcggag ggtagattgc ggcttcttca ctcaaatcga
      661 gcaccggcaa atctgatccc attgttattg gtgaccgccg gaaatttacg acattgtgaa
      721 tatagcagcc ttctacgctt tcggaaagat aggaacgtgc gtggaccgcg ttttccagtg
      781 aattcagatc aaaatggctg aacaatgacg aagaatacga ttgccgggtt catgatcccg
      841 ttgagaagct ggcgttccat tgccttgaat gtttcggctt gagtcgtcac gacaccggtt
      901 tgcgcgtgat aagcaaaaag tctcggcaaa tagaataaac cggccacccc gtcgaaggcc
      961 gcaatcagat gcacggcctt cgagtataaa agaagattgc tcatgaaatt cgtgaggctg
     1021 ttcatctcgc gcactcgagt ttgcgcgcgt caagcttccg cgcgaacgag acggataagg
     1081 cgctcgacat tctcgatagg cgtttcaggg agaataccat gaccaagatt gaagacgtat
     1141 ggtttcgacg ctagagagtc gagaatcgcc ttcacccgcc gatccatagc atccccgcct
     1201 gcgagcagca acagcggatc aagattgccc tgaaccgtca ggtgggcttg catcttcgcc
     1261 atgaccttca agggcgccgc cgtgtcgcag cccagcgcat ccacgccagt ttttgcaaca
     1321 tattcctcgt attgcggcga acttcctcgc ggaaacccga tgatcggaac gtggggatga
     1381 gccgctttca cctgcttcac gatcctcgcc gtcggctcga tgacgaaggc ctcgaagtcg
     1441 ttatccgcaa gagctccagc ccagctgtcg aagatttgca gggcacgggc gccggcgttc
     1501 gcctgcccga tcagataagc ggaggaagct tccacaagca ggtcgagaat ggcgccaaag
     1561 gttttgggtt cgcggtaggc caaaagtttc gcggccttgt agtctgtgga gccgcggcct
     1621 tccaccatgt agcttgcaac ggtccagggt gcaccgcaaa acccgatcag cggcgctctg
     1681 ccttcaaggc cgacgatggc cttatggatc gcttcgtaaa cgatctcaaa ggttgattgc
     1741 gccttggaaa cagaaagctt ggaaacatcg gagaggctct tgatgggctc cagatgcggc
     1801 ccctcccctt cacgaaattc caagtgttgg ccgagagcat ggggtacgac aaggatgtcc
     1861 gaaaagatga tggcggcatc gaatccgaag cggtggatcg gctggatggt cacttccgcg
     1921 gcaagcatcg gattatagca gaggttgaga aacgagcctg cctgcgctct gaccgcgcga
     1981 tactcggcta gatacctacc cgcttgccgc atcagccaca tgggaggagc cttgtgagcc
     2041 tttcctgcca gggcttccag gaacttcggc gtggtttgct tgacggcggc ttcggagggc
     2101 gtcttcatca aaaaaaattc cacttaagat ctgttgtttt tactgggggg aggtaacacg
     2161 tgcaaacttc atgttttgcc acggtcatcc cctggttcca gctgcgggtc cggctttgga
     2221 ttgcaagcag ctgggcttct gttaaaagct gcgcttgcat cgggttcatc ctacgcctgt
     2281 ttagccttgc tttgcccttt tcagcaatga ataactagag gaacaagctg tgaagactca
     2341 gcgcaggtca aaggcagggt gcaaacctag agcttttttt ccatagcacg atgagctgtg
     2401 cgctcttttt taattagtgt ggaggactca atggcggatg acggagccaa gccgggcctg
     2461 ggtggcaagg aggggctttc atccccggaa tccacaccaa tccccacccc gaagcacttt
     2521 catctgcacc tcgtttcgga cgcctcgggc gagaccttga cgacgatggc gaaggcggcg
     2581 gtggtacaat tgcccggggc caaacccaac cggcatatgc accctctggt tcgcaatccg
     2641 aaacagctcg caaccgtttt gcagcacatc gaatcggcgc cgggcatcgt gctttacacg
     2701 ctcatgaacc cggaccttgc cgaacttctc gaaaaaagat gtgcggagct tgaggtgccc
     2761 tgcatcttcg tcctgaagcc tgttcttcag gctttcgagc agtatttgaa aatgccggcg
     2821 cgcgctgttg tcgcaggcca gcatacgctc gacgccgact acttccgtcg gatcgaggcg
     2881 atgaacttca cgctgatcca tgatgacagc caaagcaccg aagacctgga gaaggccgac
     2941 attgtgctgg tcggcgccag ccgcacgtcg aagacaccga cgagcatcta tctcgcaaac
     3001 aggggcttca agaccgcaaa cgtgccgctc gttccgaaca taccgctgcc gcctaccctg
     3061 aaagaactca agacgccttt tgtggtcggc ctggttgcaa gcccggaacg gcttgccgag
     3121 atcaggagaa atcggttgct tgctttgaat agcaacaaat tcgatgatta tattgatgct
     3181 gatgctataa aggacgagat agcattcaca aagagactgt gcgcgcagca cggctggcag
     3241 gtccttgatg tcacgagaaa gtccatcgag gagacggctg cggccataat cgctctttac
     3301 gaaggaagcc gccgttagcc aagctgttgt ctttatcaag cttgagctca aaaaaggtta
     3361 caattcgcgc gatgcaatcc gactacccag agatcatact tgcgtccgcg agttccgcgc
     3421 ggggggccat cctggctcac gcaggcgtca gatttcatca gcgcccttcg atggtggacg
     3481 aggcggagga gcaaaagcac ttcgccggca acaccacgcc cacggaggtt gccttggcgt
     3541 tggcccggct caaggccgac agtgtcttgg agatggaccc cggggccatc gttatcggcg
     3601 cagaccaggt tctggcgatc ggaaatgaac tcttgcagaa gcccggaagc cgggaagagg
     3661 cgcgtgatca gcttctgaaa ctacggggta ggactcacta cctccattcc ggggcggtcc
     3721 tgctttacaa ggatcacgcg gcgaccttct ccgacatcgc gaccatgaca gttcgcgatt
     3781 tctcggatga gttcctcgat tggtatcttg agaccgcggg cgaaggcgtc ctcacctccg
     3841 tcggcgccta tcatattgaa ggcctcggca ttcacctttt ctcagaggtg aagggcgatt
     3901 atttcacgat cctcggtttg ccaatccttc cggtgttggc cgagctgcgc cggctgtcga
     3961 ttctcatgaa ataggagatg ccggcatgac gcgcgcttgt gtgatcggct ggcccatatc
     4021 ccacactcta tcgcccgcga tacaccgcta ttggctgaag gcatacggat tggacggcga
     4081 atatggcaag gcggccgtcg aaccgaagga gtttgaaggc tttcttatga atctggccgc
     4141 caacggcttt tccggcggca acatcacggt gccccacaag atcgaggcgc accgcttttg
     4201 cgagcggcgc gacgcggctg cggaagccat cggagccgtg aacacggttt ggctcgagag
     4261 aggcaggctg gcgggctcaa ataccgatgc attcggattc ctcgccaacc tcgacagcga
     4321 agcatcagga tgggaccgag gcggcccagc tgtggtcatc ggcgcgggcg gcgcggcgcg
     4381 cgccattatc tgggcgcttc gacagcgcgg cttcgaggac atccggatcg tcaaccggac
     4441 gaagccccgg gccgaggagt tggcggcgcc ctttccgcct gcatccgctc acggtcttga
     4501 agagttgggc ccggctctgg aaggcgcaaa tttcatcgtg aacacctcga cgttgggcat
     4561 gaatggcgct ccgccgctca atgtcgatct ttccgctgct gcgcgggaag ctacggtttg
     4621 cgacatcgtt tattcaccgc ttgcaacgga cctcttaaac gaggctcgcc gtcgtggatt
     4681 aagggccgtc gatgggctag gc
//
LOCUS       DATMGX010000594         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_383198, whole genome shotgun sequence.
ACCESSION   DATMGX010000594 DATMGX010000000
VERSION     DATMGX010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383198"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2655)
                     /locus_tag="VNR65_09700"
     CDS             complement(<1..2655)
                     /locus_tag="VNR65_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWJ18985.1"
                     /translation="MRNLPFVEAFQAVVGIAVSVIPEGLPALITITLAIGVQRMAQRN
                     AIIRRLPAVETLGSVSRICSDKTGTLTLMEMMVASVVTSESSYRVTGNGYANEGEVLR
                     ESGSTDQDQALRHMAQVSVHCNDAELRKVDGTWKVEGDPTEAALYPFAAKLGLDRQAE
                     KQGLPRIDAIPFESEHKFMATLHSASDGGQVLFVKGAPEVILEHCTLQETDGGPVALQ
                     RQYWTEASDRLAAQGERVLALAWLPKPGLQARSLGPADLPRQLVLLGLVGLMDPPRKE
                     AIEAVTECHNGGIRVTMITGDHKITAAAIAKMLGIGDGKTAVAGAEIEEMNDSALRER
                     VRDVDVFARASPEHKLRLVKAIQDNGQIVAMTGDGVNDAPALKRADIGVAMGIKGTEV
                     TKEAAAMVLADDNFASITAAVREGRTVYNNIEKALLFMLPTNVAQALVILVAIIVGFT
                     LPITAPQILWVNMVTSVALGLIISFEPHESDVMQRPPRMVSRPLLDSFAVWRVVFVGL
                     ALLALTLGAFFWMKSQGAADELARAVAVNALVIGQVFYLLNSRYKTESSFSIRAHLGN
                     RYLPLGVGAVVLLQILFTYAPPVQRLFGTASVPSYVWPWLLLGGFLFFLVVEAEKFII
                     RLRRSSQGGSSAIGSSGQRPTVPLAMNAAPLSAPGRIQGASWSSWQGLIGALAVVGLV
                     GGEVYSFWRQRDGGGYIADVAERSAMAHNISARGTIDIQTKALVKAPVSGKIEKLHCE
                     PGAKVQASQLCASINPRPDQLALDRQKSVLAAAQLQRDRDAAALARAKSEFEHQRTLV
                     GRAAVSPAALARSRSGLEHARAEAEASEAALTRSQAAFEEAQSDLGLTPVLSPTDGTV
                     LSRGAEIGQWVDSGKDQALFVIAPVSASL"
BASE COUNT          535 a          858 c          791 g          506 t
ORIGIN      
        1 cagtgacgcc gagaccggcg cgatcacaaa cagagcctga tccttgcctg aatcgaccca
       61 ctggccaatt tcagctccgc gcgaaaggac cgtgccatca gttggcgaaa gcaccggggt
      121 aaggccgaga tcggattgtg cctcctcaaa agcggcttgg cttcgggtaa gcgccgcttc
      181 agacgcttcc gcctccgcgc gcgcgtgttc aaggccagag cgggatcgcg ccagcgcggc
      241 aggagagacg gcggcgcgtc ccaccaaggt ccgctgatgt tcgaattcac ttttcgcgcg
      301 cgcgagcgcg gccgcgtctc tgtcacgttg cagttgcgcg gccgcaagca cgcttttctg
      361 gcgatccagc gcaagttgat cgggccgagg gtttatgctc gcgcaaagtt gactcgcctg
      421 aaccttggcg ccgggttcac agtgaagctt ttctattttg ccggaaactg gggctttgac
      481 caatgccttg gtctgtatat caatcgttcc tctcgcgctt atgttatgcg ccatcgcact
      541 acgctccgcg acatccgcaa tgtatccgcc gccgtctctt tggcgccaaa atgagtaaac
      601 ctctccgccg accagaccta ccacggcgag agcgccaatc aatccttgcc acgagctcca
      661 ggacgcgcct tgaattcgac caggcgcgct tagcggggcg gcgttcatcg ccagagggac
      721 cgtcggacgc tgtccgcttg agccgatcgc cgaggagccg ccctgggatg aacgtctgag
      781 gcgtatgatg aatttttccg cctccacgac caaaaagaag aggaagccgc ccaacaacag
      841 ccatggccag acataggacg gcacggatgc ggtgccgaac aagcgctgga cgggcggcgc
      901 ataagtgaac aggatctgca gcagaaccac tgctccgacg ccaagcggca aatagcgatt
      961 gccgagatgc gcccgtatcg agaacgagga ttccgtcttg tagcgactgt tgagcaggta
     1021 gaacacctgg ccaatcacca gcgcgttcac agcgacggcg cgtgccagtt cgtcggcggc
     1081 gccttgagac ttcatccaga agaaagcgcc aagtgtcagc gccaggagcg ccaatccgac
     1141 aaaaacgacg cgccacacgg cgaagctgtc gagaagcggg cgcgagacca tgcgcggcgg
     1201 acgctgcatc acgtcacttt cgtggggttc aaacgagatg atcagcccca gcgccaccga
     1261 ggtcaccata ttgacccaca aaatctgggg cgccgtaatc ggaagagtaa agccgacaat
     1321 gatcgccaca aggatgacca gcgcctgagc gacgttggtc ggcagcatga agagaagcgc
     1381 cttctcgatg ttgttgtaaa ctgttcgccc ttcacgcacg gctgcggtta tagacgcaaa
     1441 attatcgtcg gcgagcacca tcgcggccgc ctccttggtg acctcggtgc ctttgattcc
     1501 catggcgact ccgatatcgg cccgcttcag ggccggcgcg tcgtttacgc catcgccggt
     1561 catcgcaacg atttgcccgt tgtcttggat agccttcacc agacgcagct tatgctcggg
     1621 gctcgcgcga gcgaacacgt cgacgtcgcg cactcgctcc cgcagcgcgc tgtcgttcat
     1681 ctcctcgatc tcagccccag cgaccgccgt cttcccgtcg ccaatgccca acatcttcgc
     1741 gatcgccgcg gccgtgatct tgtgatcgcc cgtgatcatt gtcacgcgaa tgccgccgtt
     1801 gtgacattcg gtcaccgcct cgatagcttc cttgcgcggc ggatccatga ggccgacgag
     1861 tccaagcaga accaactgcc tgggcagatc cgccgggccg aggctccgcg cctggaggcc
     1921 tggctttggc agccatgcga gcgcgagtac gcgttcgcct tgcgccgcca gacggtccga
     1981 cgcctccgtc caatattggc gttgcagggc gacgggcccg ccgtcggtct cctgtaaggt
     2041 gcaatgctcg agtatgactt ccggcgcgcc cttgacaaaa agaacctgcc cgccgtcgga
     2101 cgcggaatgc aaggtcgcca tgaacttatg ctcggactcg aacggaatgg cgtcgatccg
     2161 cggaagcccc tgcttttcag cttgtcgatc taagccgagt ttggcggcga acgggtatag
     2221 cgccgcctct gtcggatcgc cttccacttt ccaggttcca tccaccttgc ggagttccgc
     2281 gtcattgcag tgaacggaga cttgcgccat gtggcgcagc gcctggtcct gatccgtgga
     2341 accgctttcg cgcaggacct cgccctcgtt ggcgtagcca ttgccggtga cgcgatacga
     2401 ggattcagag gtcacgacgg aagcgaccat catttccatg agcgtgagcg tgccggtctt
     2461 gtcggagcag atccgcgaaa cagagcctag cgtctccacg gccggaagac gccgaatgat
     2521 tgcattccgt tgcgccatgc gttgtacgcc aatcgccagc gttatcgtta tgagcgctgg
     2581 caacccctct ggaatgacgg acacggcgat gccgaccacg gcctgaaacg cttccacgaa
     2641 aggcaagttc cgcacccatc ttccataggc gaagaccagc acgctgacga
//
LOCUS       DATMGX010000595        13608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_79752, whole genome shotgun sequence.
ACCESSION   DATMGX010000595 DATMGX010000000
VERSION     DATMGX010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 13608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13608
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79752"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VNR65_09705"
     CDS             complement(<1..949)
                     /locus_tag="VNR65_09705"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HWJ18986.1"
                     /translation="MRIHIPALIALIGLSALPASAQTSYPNQRPITIIVPFAAGSGTD
                     TLARILTASMAEGEFKKATFVIENRPGADGIIGASAAAKAAPDGYTLFLTTNTTHSVN
                     PYIHKTLPYNPNADFVPIGLLGETAPALLTAADNPAATVKDVVELVKQKPGDLNFAAS
                     NTSSLASTQMLERRIGGKVVIVKYKAAPEALTDTMTGRINYFFGDLASGGSLVRGGKL
                     KALAVLSDRRLPGFDQVPTMAEAGYPGMEIPIWIGMFAPKGTPPEIIERISRALVAAQ
                     GTQKFIDALARGAVNVRTTTPAVFAKFVADQYQMWGRLAK"
     gene            complement(1080..1631)
                     /locus_tag="VNR65_09710"
     CDS             complement(1080..1631)
                     /locus_tag="VNR65_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWJ18987.1"
                     /translation="MAAIAIFLVAFSDGTQAFELTSPDVRPDQPLPEKFTFSGLGCQG
                     QNISPALSWTNPPEGAKSFALMVHDPDAVTGGAGVWHWVVINIPATARSIEQGAGTAD
                     GAKLPEGSRQINNDYLGFINSPGWGGPCPPKGQKAHSYNFTLYALKVEKLNLPPNATG
                     SHAGFVVNLNALDKAKLTMTYGR"
     gene            1935..3119
                     /locus_tag="VNR65_09715"
     CDS             1935..3119
                     /locus_tag="VNR65_09715"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWJ18988.1"
                     /translation="MTGQSDVWPIDAELASGPLRGVRILDLTTVVMGPFATQMLGDLG
                     ADVVKIESQAGDSMRWIGPWRHEGMGPLFLQANRNKRSVVLDLKTAEGKQALIALANR
                     ADVLVSNVRPQALQRLGLDYDSIRAGNERIIYCAAVGYGSGGPDAGKAVYDDLMQAAS
                     GIAGLFGAVDGAPRYAPINICDRVVGLYVANAISSALYHRAMTGEGQAIEVPMFETMA
                     QFVLADHMGGGAFKPPLGEMGYKRLLSRTRGPYATRDGHISLVVYTDQHWRDFTQLVG
                     CPTLMDTDDRFRSQESRTRYAEDIGRFLSDHLPARKNEEWLKVLHEIDIPACPVNSIG
                     DLFDDPHLKAVHFFEEMNHPTEETVMVCRHPVQFSRTPASVRRLAPNLGEHTAEVLGN
                     KT"
     gene            complement(3229..3972)
                     /locus_tag="VNR65_09720"
     CDS             complement(3229..3972)
                     /locus_tag="VNR65_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWJ18989.1"
                     /translation="MSKIVERTLDLLELFARERRPLTLSDIAHLLKIPLSSCHDVVQA
                     MQARGYIYELAPRAGYYPTSRLHLLGKEIGNNDPVLMRAELLLRSLRDTFDESVLLSK
                     VGGLTATYLLAFEPTHPLRMLVKVGDTIRSLHATSAGKALLSSLDEHTLTAFLKSEKL
                     LPLTKQTITSKTVLRKDIDLGKKRRWFLNRGESLNGVTTVSAPFKWNSSIYIVTIAGP
                     SSRLDQKLEKTVDLLTETCQLLEMQQAAA"
     gene            4346..5524
                     /locus_tag="VNR65_09725"
     CDS             4346..5524
                     /locus_tag="VNR65_09725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ18990.1"
                     /translation="MKRFEPLATTPERQEICTAIRALCDNFDDGYWAEKDRAHQFPFE
                     FAKAVADGGWLGIAMPTKFGGAGLGVTEAAIMMQEIGRSAGVFAACSTVHINIFGLHS
                     IVRHGTDKQRAAWLPSVINGTSRACFGVTEPDAGLDTSRIKTRAVRTGDQYLVNGQKI
                     WTSAAQQADKIVLLARTTPIDECARPTDGLSLFYTDLDRSAVEIHEIQKMGRHAVNSN
                     QVFFNDLRVPVECRIGDEGKGFRYILDSLNPERILNAAEVVGIGRRALEKAAAYAGER
                     VVFGRKIGQNQSIQHPLAECWAELYAAELMTLHAAELYDAGKPCGAEANAAKLLAADA
                     GFRACDRAIRTHGGMGYAADYHVERYFREMVAMQLAPVSREMILCFIAERELGLPKSY
                     "
     gene            5544..6347
                     /locus_tag="VNR65_09730"
     CDS             5544..6347
                     /locus_tag="VNR65_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWJ18991.1"
                     /translation="MTENNDKPDIRTERRGQSLWIWIDREERRNAINKRVISGIQKAV
                     TEAQQDPVLRGIVLTGAGRKAFCAGADLGGGTETFTLGLDEPMTDFGKLARVVRNIGI
                     PIIGRINGDCVAGGMGLMSLCDLVVVADHARFGLPEAKVGVFPMQVLVFLRGMIGARH
                     INELCLTGELISATRAREIGIANYVVPFEELDARVDALMSKIGEMSPVALRRGKYAIA
                     AMESMAFAEAMAFAETMISVTSLTNDAAEGIAAFNERRPPKWRQKVNDA"
     gene            6340..8142
                     /locus_tag="VNR65_09735"
     CDS             6340..8142
                     /locus_tag="VNR65_09735"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HWJ18992.1"
                     /translation="MPEKLVRIGGASGAWGDSPGALGQLVGGGVDYLMMDYLAEVTMS
                     LLARARMKDPHAGYPPDFIAYLKSVLPQIAKQGVKVSTNAGGVNPSGCKKALEAVIAE
                     LGLSLRVAVVEGDDVMLLTDRLRAGGLREAVSGDRLPDRLLTANAYLGAIPIKAAFDG
                     GADIVITGRCADSALALGILMHEFGWAADDYDRLGAGSLVGHILECGPQATGGTFSDW
                     ETVPDWHNIGYPIAECQSDGSFVVSKPDGTGGLIVPGVIAEQALYEIGDPAAYLLPDV
                     TADFSNVRLTQVGPDKVLVENARGRPPTNQYKVSATYQDGYRAVATVSIVGPNAALKA
                     ERTGKTLIDRARMIFKQKGLPDFTATHIEALGAEASYGAKSATRASREVLLRLVVEHA
                     DRGALEILARELGSVGLSCAPGTTGIYTGRPKPTPVVRLFTFFLDKSLLGRPRIQIGD
                     GPMMDVDVPFQGGYAPAKPTTVPTADIVPNETTIELPLARLAYARSGDKGNSANIAII
                     ARKPKYVPLLRRELTPERILEHLGHLVGGPAVRFEAPGLNALNFLISDALGGGGMASS
                     RIDPQGKAYGQMALEMIVNVPKSWAEELAVYETA"
     gene            8331..9362
                     /locus_tag="VNR65_09740"
     CDS             8331..9362
                     /locus_tag="VNR65_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ18993.1"
                     /translation="MRAIGRNIVAATFAVTLVVSPAAFGADAKPEKADVKIAVGGKSF
                     MIYLPLTLTERLGYFKDAGLNVEIIDFGSGTKSLQALVGGNVDATTGSFDHTIQMQAK
                     GQPILAVVETGRYPGIVLGLVTSKKLDYRSPKDLKGLKIGVTAPGSQTNFMVNYILTQ
                     NGIKPEDVSFIGVGGPTTAVAAVKKGEIDALSHADPAITQVEMSGELKAIFDTRTTAG
                     TQAVFGGEYPSSVIYVSPAFAEQNPNTVQAIVTATVRTLRWIAKSTPEEIAKAMPPEY
                     QGKDLALYVEIVRKSKAMWPADGRINKAGAENAYHVLSAFDKAVSAAKIDIGKTYTDR
                     FVDKALAAH"
     gene            9413..10819
                     /gene="leuC"
                     /locus_tag="VNR65_09745"
     CDS             9413..10819
                     /gene="leuC"
                     /locus_tag="VNR65_09745"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWJ18994.1"
                     /translation="MPGRTLFEKIWAQHVVAQLSNDLSLLSIDRHYLHDLEGGPNFIR
                     LAKLGYRVHDPDLTYAMPDHAIASSPGRTTDTNPIGGRLLREMRRGAFASGIKLFDIG
                     DEGQGIVHVAAPEMGLTLPGATVVCPDSHTCTHGAFGALAIAIGSSEVAHVLATQTLR
                     QKKPKMMRVHFNGSPAPGVTAKDVILATIGRLGTAVGRGYAVEYAGALVGELTIEERL
                     TVCNLSIELGSKTGIMAPDDTTYEYLAGLPYAPKNALFDRAVAQWRQLPSDEDAIFDA
                     DHSINVADIAPQITWGTSPQDVIDITGRIPDPSNETNAERRKSMDAALKYMGLRPGDA
                     IEGTPIDWVFIGSCTNGRLSDLREAAKALVGRKVAANIRAWVVPGSVAVQRAAEREGL
                     HRQFRDAGFEWREPGCSMCLGANGDIIAPGERSVSTTNRNFVGRQGAGARTHLASPAM
                     AVAAAVAGCIADVRKLGH"
     gene            10822..12552
                     /gene="leuB"
                     /locus_tag="VNR65_09750"
     CDS             10822..12552
                     /gene="leuB"
                     /locus_tag="VNR65_09750"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWJ18995.1"
                     /translation="MNKFTRVTGAAAPLMRINIDTEVVIPINRLIGHKRGELGPYCFE
                     AWRYALDGRENPEFVLNQPHYREAKFLLAGDNFGCGSSREHAVWSLADFGIRCIIAPS
                     FGDIFYWNCFQNGMLPIRLPDDQVKELAAEIEAAEQPLVTVDLETQAITMPSGRQVTF
                     TTDPERRAALLEGLDEIGMTLKLDAQIRGHQSRDRESRPWIYRREETARVARLLILAG
                     DGIGPEILAEVRKVAEWFIAKRQLSVELREELFGISAWKTHGDLMRQQTWDEIVAADA
                     ILFGATGSPEYEKIPAAARKVDQLLRMRKELDLFCNLRPVRTLPALGASSSLRPEVIA
                     DCDMMIVRELCGGIYFGKPRGREKLNTGGERAVNTCMYTTGEIERIARAAFDLARVRS
                     GRVCSVDKSNVLTETGGLWRDTVQALQDRHYPDIELSHMYVDNCTMQLVRAPKQFDVL
                     VTENLFGDILSDCAAMVAGSLGMLPSASLGPVRPDGGRHALYEPIHGSAPDIAGRGIA
                     NPLGAIMSFALCLRFSLGLAQEADRLERAVDAAISHGARTADIMQPGEKSISSKAMGD
                     AVLAELEQMQ"
     gene            12888..13124
                     /locus_tag="VNR65_09755"
     CDS             12888..13124
                     /locus_tag="VNR65_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18996.1"
                     /translation="MSAYDPKRTLGVHRGNDFDADFSPYQFTRLLACLFAFNKRNENP
                     TQNRNRNACGDNLRPIDVALRHVFLLGIAPVDVA"
BASE COUNT         2924 a         3781 c         4035 g         2868 t
ORIGIN      
        1 ctttcgcgag gcgtccccac atctgatatt ggtcggccac gaatttggcg aagactgcag
       61 gcgttgtggt tcggacattg accgcgcctc gcgccaaggc gtcgatgaat ttctgcgtgc
      121 cttgagccgc aaccaaggcg cggctaatgc gttcgataat ttccgggggc gttcctttcg
      181 gcgcgaacat accaatccag atcggaattt ccattccggg atacccggcc tcggccatgg
      241 tcggtacctg atcaaagccc ggcagccggc gatcgctcaa cacagcaagc gcctttagtt
      301 ttccaccccg caccagcgat ccgcctgagg caagatcgcc gaaaaaatag ttgatcctac
      361 cggtcatggt gtccgtcagt gcctccggcg cggctttgta tttgacgatc acgaccttgc
      421 cgcctatgcg tcgctcaagc atctgggtcg aagcaaggct ggaagtgttg ctcgccgcga
      481 aattcaggtc tcccggcttc tgtttgacca actccacaac atctttgact gtcgccgccg
      541 ggttgtcggc cgctgtcaga agagctggcg ccgtttcccc gagcaatcca atcggaacaa
      601 agtccgcgtt tgggttgtac ggaagcgtct tgtggatgta agggttgacc gaatgtgtgg
      661 tgttggtcgt gaggaacaac gtgtatccat ctggggcggc cttggctgct gccgacgcgc
      721 caatgatgcc atcggcgcct ggccggtttt caatgacaaa ggtagccttc ttaaattctc
      781 cttccgccat gctggcagtc aatatgcgcg cgagcgtgtc agtgccgctg cctgcggcaa
      841 aaggcacgat gattgtgatc ggcctttgat tgggatagct agtctgagcg ctcgcgggca
      901 gcgcgctcag tccaatcaag gcaatgagtg ccgggatgtg tattctcatt tgcgcctcct
      961 atttgtcatt tgtcagttct gtctgaccgg gcgtgctgtg gcactgttat atggagcgaa
     1021 agtgcagaga ttgaatccaa gccgtcgcgc gcgaaaatcg acacttagcg ccaccattcc
     1081 tagcggccgt aagtcattgt cagtttggcc ttgtccaacg cattgagatt caccacgaag
     1141 ccggcatgtg agcccgtggc gttcggcggc aagttcaatt tttcgacctt gagcgcatac
     1201 agagtgaagt tgtagctgtg cgccttttgt cccttgggcg ggcaaggccc gccccagccc
     1261 ggcgagttga taaatcccaa atagtcgttg ttgatctgac ggctgccttc cggcagcttg
     1321 gcaccgtcgg ccgttccggc accctgctca atggagcggg cggtggcggg aatattgatg
     1381 actacccagt gccagacgcc agcaccgccg gtcacggcat ctgggtcatg caccatcaaa
     1441 gcaaagctct tggcgccttc gggcggattc gtccagctta gtgcgggcga aatattctgc
     1501 ccttggcagc cgagaccact gaaggtgaat ttttcgggca aaggttggtc aggcctaacg
     1561 tcggggctgg tgagctcgaa agcttgcgtg ccgtcgctga atgctacgag aaagatggcg
     1621 atcgcggcca agtattttgc tcgacgcact gaaattacgc gggacatgga tttgcgattc
     1681 gatctcatgt ggacgctttc ccgatatggt cgccggcgct ctcgactgca aacaaaatat
     1741 tcgcaatagc gaagataaat tcgacatagc gaataatcta cgatatcatg ggtgtgtcaa
     1801 ggtggaatcc gccgtgacga tttgggctgc ggaattgatc gccccttggg aataatgttg
     1861 ccactgcgaa gtcgctcggc gcgcttgatg cttcgcggca atcatccgcc ggccaataag
     1921 agaaaggctt ctccatgacg ggccaatcag atgtttggcc aattgacgcc gagctggcaa
     1981 gcggcccgct gcgcggggtg cgcatcctgg atcttacgac tgttgtgatg gggccatttg
     2041 ccactcagat gcttggcgat ctaggcgccg atgtggtcaa gatcgaatcg caagcgggcg
     2101 attcaatgcg ctggatcgga ccctggcggc acgagggcat gggtcctttg tttttgcagg
     2161 ccaaccgcaa caagcgcagc gtcgttcttg atctcaagac tgcggaaggc aagcaggccc
     2221 tcatagcgtt ggcgaaccgg gcggacgtgc tcgtgtccaa cgtccggcca caagccctgc
     2281 agcgtctcgg cctagactat gacagcatcc gcgcgggcaa cgaacggatc atttattgcg
     2341 cggcggtcgg ctacggaagc ggcggacctg atgcgggaaa ggcggtttac gatgacctga
     2401 tgcaagcggc ttccggcatc gccggcttgt tcggtgccgt ggatggagca ccgcgttacg
     2461 ctcccatcaa tatttgcgac cgagtggtag gactctatgt tgccaatgca atcagcagtg
     2521 ccctgtatca tcgggcaatg acgggcgagg gccaggcgat tgaggtcccg atgtttgaaa
     2581 ccatggcgca attcgttttg gccgatcata tgggcggcgg ggcattcaag ccgccattgg
     2641 gcgagatggg gtacaaacgc ctgttgtctc ggacgcgcgg accgtatgcg acacgcgacg
     2701 gccatatctc actcgtggtt tacaccgacc agcactggcg cgacttcacc caacttgttg
     2761 gctgtcccac actcatggat acagacgacc gctttcgctc acaggaaagc cggacacgtt
     2821 acgcggaaga tatcggccgg tttctttccg atcacctccc agcgcgtaaa aacgaggaat
     2881 ggcttaaggt tctgcatgaa atcgatattc cggcgtgccc ggtaaactca attggcgatc
     2941 tattcgatga tccgcatcta aaggcagttc acttcttcga agagatgaac cacccgaccg
     3001 aggagactgt catggtctgc cgccatccgg tccagtttag ccgcacgcct gcctccgtcc
     3061 ggcggctggc gccgaaccta ggtgaacaca ccgcagaggt attgggtaac aagacgtagg
     3121 cctttgagcc gcgatcccgt taaacctcaa agaattaaat ggccgttagc tggcggttca
     3181 actcggcagg tcgtgctgac gcagttactc ttaagtcgtg cccctggctc aggcggcggc
     3241 ttgctgcatt tcgaggagct ggcaggtttc ggtcaggaga tcgactgtct tttctaattt
     3301 ttgatcgagc cgggacgatg ggcccgcaat cgttacaatg taaatcgatg aattccactt
     3361 aaatggtgcg gaaacggtgg tcactccgtt caagctttcg ccacggttaa gaaaccaacg
     3421 tctttttttg ccgaggtcta tgtccttacg aagcaccgtc ttagacgtga tggtttgctt
     3481 tgttagcggc agaagttttt ccgacttaag aaatgcggtg agggtgtgct cgtccaaact
     3541 gctcaaaagc gcctttcctg ctgaggtcgc gtgcaggctg cgtatagtgt cgccaacctt
     3601 aacaagcatt cgtagtggat gggttggttc gaatgccaac aggtaggtgg cagtcaatcc
     3661 accgaccttg gacaacaaaa cagattcatc aaaagtgtcg cgcaacgatc gaagcaacaa
     3721 ctctgctcgc atcaagacag gatcattgtt tccaatctcc ttaccgagca agtgaagtcg
     3781 cgatgtcggg taatagccgg cccgcggtgc cagttcataa atatagccgc gagcttgcat
     3841 ggcctgcacg acatcatggc agctggagag tgggattttc agcagatgcg cgatatctga
     3901 aagtgtcagc ggtcgccttt ctctcgcgaa tagctccaaa agatccagtg tgcgctcaac
     3961 aatcttcgac atccaacgtt tctccgctat ctcaacacgt gagatagcaa actatattct
     4021 tgatttcggc ggtagcaatc gatgttcggg agggctgatc ttttccgcct aagggtggcc
     4081 agctccaaat atgattcatc gccttgacgt ggtgaataag caagcttaaa gtttcgcatt
     4141 agcgaattat aattcgctat agcgtattga attgatagat ggcgacgcac gggcctaagg
     4201 ctgagacaga ggacgatgtc aggtgccgcc catacaatgg acctccggca gggcatattc
     4261 agccgcagca acccaccgaa gtttccgata ttccaacgaa tagggaatag gaatatcggg
     4321 ccggccaagc caaaggagcg caggtgtgaa acgtttcgaa cccctagcga ccacaccgga
     4381 acggcaggaa atatgcaccg ccatccgggc gctatgcgat aacttcgacg acggttactg
     4441 ggctgagaag gatcgggccc accaatttcc atttgaattt gccaaggctg ttgccgatgg
     4501 cgggtggctg ggcatcgcga tgccaacaaa attcggcggg gccggactag gcgttaccga
     4561 agccgccatt atgatgcagg agatcgggcg ctcggctggc gtttttgccg catgctcgac
     4621 ggtccacatc aacatttttg gcctgcactc aatcgtcagg cacggcaccg ataagcagcg
     4681 tgccgcgtgg ctgccgtctg tcatcaacgg gacatcgcgc gcctgttttg gcgtgacgga
     4741 acccgatgcg ggcctcgata cgtcacggat taaaactcgt gctgtgcgaa cgggtgatca
     4801 atatctcgtc aatggtcaga agatctggac cagcgctgct cagcaggcag acaagatcgt
     4861 cttgcttgcg cgcacgacac cgatcgatga atgcgcacgg ccgactgacg gtctttcgct
     4921 attctatacc gacctggacc gctccgccgt cgaaatccac gagatccaaa aaatggggcg
     4981 gcacgcggtc aattcgaacc aggtgttctt caatgatctc agagtacctg tcgaatgtcg
     5041 cattggggac gagggcaaag ggttccgcta tattctcgat agcttgaatc cagagcggat
     5101 tcttaacgca gccgaggtcg ttgggatcgg tcggcgcgca ctagaaaaag cggccgccta
     5161 cgcaggcgag cgcgtcgtat tcggacgaaa gatcggacag aatcagtcga ttcagcatcc
     5221 gctcgcggag tgctgggcag aattgtacgc cgccgaactc atgaccctgc atgcggccga
     5281 gttgtacgac gcgggcaagc cttgcggcgc agaggcaaac gccgcgaaac ttctcgcggc
     5341 cgacgcggga tttcgcgctt gtgatcgagc catccgtact cacggcggca tgggctacgc
     5401 cgcggactac cacgttgaaa gatacttccg cgaaatggtc gcaatgcagt tagcgccggt
     5461 atcgcgcgag atgatcctat gtttcatcgc agagcgcgag cttgggctac ctaaatccta
     5521 ctgagaaaag cgagagatcg ttcatgacgg aaaacaacga caagcccgac atccgaacgg
     5581 agaggcgtgg acagtctctt tggatttgga ttgaccggga ggagcggcgc aacgcgatca
     5641 acaagcgggt catttccggc attcagaaag cggttacgga ggcgcagcaa gacccggtgt
     5701 tgcggggcat cgtgcttact ggcgcaggcc ggaaggcgtt ttgcgccggc gctgacctcg
     5761 gtggcggcac tgaaactttt acgctcggcc ttgatgagcc gatgacagat tttggcaagc
     5821 ttgcccgcgt cgtccgcaat atcggtatcc cgatcattgg ccgtatcaat ggcgattgcg
     5881 tcgctggtgg tatgggcctg atgtcgctct gcgatctcgt cgttgtcgcc gaccatgcgc
     5941 ggttcgggtt gccggaggcg aaggtcggcg tatttcctat gcaagtgctg gtctttctgc
     6001 gcggcatgat cggcgcgcgg cacatcaatg agctgtgcct gaccggcgag ctgatcagcg
     6061 ccacccgcgc gagggaaatt ggcatcgcaa actacgtcgt gccgtttgag gagctggacg
     6121 cacgggtcga tgcactcatg agcaagatcg gggagatgtc gccggtggct ctacgtcgcg
     6181 gcaaatatgc cattgccgcc atggagagca tggcctttgc cgaggctatg gcattcgcgg
     6241 aaacaatgat ttccgtcacc tcgctcacca atgatgcagc cgaaggaatt gccgcattca
     6301 acgagcggcg tccgccaaag tggaggcaga aggtcaacga tgcctgagaa actcgtacga
     6361 atcggcgggg catccggagc ttggggcgac agtcccgggg cgcttggaca actcgtcggc
     6421 ggcggcgtcg actacctgat gatggattac ctggcagaag tgacgatgtc gctcctggct
     6481 cgcgcccgca tgaaagatcc gcatgctggc tatccacccg atttcatcgc ctatctgaaa
     6541 agcgtgctgc cgcagatcgc aaagcagggc gtgaaggttt cgacgaatgc aggcggcgtc
     6601 aatccatccg gctgcaagaa ggctctggaa gccgtcatcg ccgaacttgg tctttcactg
     6661 cgggtggcag tcgtggaagg tgatgacgtc atgctgctga ccgatcggct tcgagccggc
     6721 ggtctgcggg aggcggtgtc cggcgatcgt ttgccagatc ggctgctgac agcgaacgcc
     6781 tatctcggtg cgatcccgat aaaggcagcg ttcgacggcg gtgcggacat cgtcattact
     6841 gggcgatgcg ctgacagtgc gcttgcactc ggtatactga tgcacgaatt cggctgggct
     6901 gccgatgact acgatcggct cggagccggt agtcttgttg gccatattct ggagtgcggt
     6961 ccgcaggcga caggcggaac gttcagcgac tgggagacgg tgcctgattg gcacaatatc
     7021 gggtacccaa tcgcggaatg ccaaagcgac ggcagctttg tcgtgtcaaa gccagacggc
     7081 accggaggcc tgatcgtccc aggggtgatt gccgaacagg cgctctacga gatcggtgat
     7141 cccgccgctt atctattgcc agatgtgaca gccgattttt cgaatgttcg cctgacgcag
     7201 gttggtcccg acaaggtgct cgtggaaaat gcacgcggac gtccgccgac gaaccaatat
     7261 aaagtatccg caacatatca ggacggctac cgcgcggtcg caaccgtgtc gatcgtcggg
     7321 ccgaatgcag cgctgaaagc cgagcgaacc ggaaagaccc tgattgaccg cgcacggatg
     7381 atattcaagc agaagggctt gccggatttc acggcgaccc acattgaggc gctcggcgct
     7441 gaagcgtctt atggcgcgaa gtcggcaacc cgcgcatcgc gggaggtctt gctgagattg
     7501 gtcgttgagc atgccgaccg cggagcgctc gagattttag ctcgcgaact tggctcggtt
     7561 ggattgagtt gcgcgcccgg aacgactggc atctataccg ggcgtcctaa gccaacgccc
     7621 gttgtgcgac ttttcacctt ttttttggac aagagtttgc ttggacgccc acggattcag
     7681 atcggcgatg gtccgatgat ggatgtcgat gtgccatttc aaggtggata tgcacccgcc
     7741 aaaccgacaa ccgtcccgac agcggacata gtgccaaacg aaacgacaat cgagctgccg
     7801 ctggctcggc ttgcatatgc gcgatccggc gacaaaggaa attcggcaaa tatcgcaatc
     7861 atcgcacgca agccaaaata cgtgcctttg ctgcgtcgag agcttacgcc ggagcgcata
     7921 ctagagcacc ttggtcactt agttgggggg ccggcagtac ggttcgaggc ccccggtctc
     7981 aatgcactga atttcttaat cagtgacgcg ctcggcggcg gcggtatggc atcaagccgt
     8041 atcgatccac agggcaaggc atatggccag atggcgctcg aaatgatcgt caatgtgccg
     8101 aagtcgtggg ccgaggagtt ggctgtgtat gaaaccgcct agcggccgaa aagcttgcgc
     8161 gagaggcgca cgactcaaat gcgggcattc cgatttagtt cggcggcgct cgtcgcgatc
     8221 aaaccagcaa aaatatgcca caactattga ctagcattcg gccgtccggt gtcatctggt
     8281 gtttgtggag ctagccgaaa agtaaatgca ttcaaccaag gataagtgcg atgcgagcta
     8341 tcggtcggaa tattgttgcg gcgacgtttg ccgtcacgct cgtggtatcg ccagccgctt
     8401 tcggcgcaga cgccaaacct gaaaaagccg atgtcaaaat cgcggttggc ggcaagtcct
     8461 ttatgatcta cctgcccctc acactcacgg aacgcctcgg ctacttcaag gacgcgggac
     8521 ttaacgtcga gattattgac tttggcagcg gcacgaagag cctgcaggcg ctggtcggcg
     8581 gcaatgtcga tgccaccact ggttcgttcg atcacactat ccagatgcag gccaaagggc
     8641 agcccatttt ggcggtggtc gaaactggtc gttatcccgg catcgtgctc ggcttggtga
     8701 catcgaagaa gctcgactat cgcagcccta aagacttgaa agggctcaag atcggcgtca
     8761 cagcgcctgg ctcgcaaacc aacttcatgg tcaactatat tctgacgcag aacggcataa
     8821 agccggagga tgtttcgttt attggcgttg gcggcccaac aaccgccgtg gccgcggtca
     8881 agaagggcga gattgacgca ttgtcacacg ccgatccggc gatcacacaa gttgaaatgt
     8941 cgggtgagtt gaaggcgatc tttgacacgc gcacgacggc cggcacccag gccgtgttcg
     9001 gcggtgaata cccgtccagc gtcatttacg tcagccctgc attcgccgag cagaatccga
     9061 acacagtgca ggccattgtg accgccaccg tgcgcacctt gcgctggatc gccaaatcga
     9121 caccggagga aatcgccaag gcgatgccgc cggaatacca gggcaaagat ctcgcgctgt
     9181 atgtcgaaat cgtcagaaaa tcgaaggcga tgtggccggc cgatggccgc atcaataaag
     9241 cgggcgctga gaacgcttac catgtattga gcgcattcga taaggccgta agcgcggcca
     9301 agatcgacat cggcaagacc tacaccgatc gctttgtcga caaggcgctg gccgcccact
     9361 aagcgctcgc acggcggcgt cacgggccgc cgtgctgata cagggaccat tcatgccggg
     9421 ccgaacacta tttgaaaaaa tctgggcgca acacgtcgtt gcgcaattgt cgaacgatct
     9481 gagcctgctg tcgatcgatc ggcattatct ccacgatctc gaaggtggcc cgaatttcat
     9541 acgcttggca aagctcggct accgcgtgca cgaccccgac ttgacctatg cgatgcccga
     9601 ccatgcgatc gccagttcgc cggggcgcac taccgacacc aatcccatcg gcggacggct
     9661 tctgcgcgaa atgcgccgtg gcgcgtttgc ctccggcatc aagctgttcg atatcggcga
     9721 cgagggccag ggcatcgtgc atgtcgcggc gccggaaatg ggtttgacgt tgcccggcgc
     9781 caccgttgtc tgcccggaca gtcatacctg cacgcacggc gccttcggtg cgctcgccat
     9841 cgccattggc tccagcgaag tcgctcacgt tctggccacg cagacgcttc ggcagaaaaa
     9901 gccgaagatg atgcgagttc acttcaatgg ctcgcccgcg ccaggcgtca cggcaaaaga
     9961 cgttattttg gcgacgatcg gacgactcgg caccgctgtc gggcgtggct atgcggtgga
    10021 atatgcgggt gcgcttgtcg gcgagctgac aatcgaagaa cggctcacgg tctgcaatct
    10081 gtcgatcgag ttgggctcca agacgggaat catggcgccg gacgacacaa cttacgaata
    10141 tctcgccggc ctgccctatg cgccgaagaa tgccttgttc gatcgggccg tcgcgcaatg
    10201 gcgccagctg ccgagcgacg aggacgctat attcgacgcc gatcacagca tcaatgtggc
    10261 cgacatcgca ccgcagatca cctggggtac cagcccgcag gatgtgatcg acatcactgg
    10321 gcggattccc gatccatcga acgaaaccaa tgccgagcgc cgcaaaagca tggatgcagc
    10381 gctcaaatat atggggctac ggccgggcga tgcgattgaa ggcacgccga tcgattgggt
    10441 cttcatcggc tcatgcacca atgggcgctt gtcggatctg cgtgaggcag ccaaggcgct
    10501 cgtcggccgc aaggtagcgg cgaacattcg cgcctgggtc gtgcccggtt ctgtagcagt
    10561 gcagcgcgcc gcggaacgcg aaggcctgca tcgccaattt cgcgacgcgg gcttcgaatg
    10621 gcgcgaaccc ggctgttcca tgtgtctcgg cgccaacggc gatatcatcg cgccaggcga
    10681 acggtcggtt tccaccacca accgcaactt cgtcgggcgg cagggagcgg gcgcccgtac
    10741 ccatctggcg agcccggcca tggccgttgc cgctgcggtc gccgggtgca tcgcagatgt
    10801 acgaaagctc ggccattgac gatgaacaaa tttacacggg tgacaggagc cgccgctccg
    10861 ctgatgcgga tcaatatcga cactgaagtc gtcattccca tcaatcggct gatcggacat
    10921 aagcgcggcg agcttggtcc ctattgcttt gaagcctggc gctatgcgct ggacggccgc
    10981 gaaaaccctg aattcgtgct caatcaaccg cactatcgcg aggccaagtt tcttttagcc
    11041 ggggataatt tcggctgtgg ctcctctcgc gagcatgccg tttggtcgct ggcggatttc
    11101 ggaattcgat gcattatcgc accaagtttt ggcgatattt tctattggaa ttgttttcag
    11161 aacggcatgc tgccgatacg cttgccagat gaccaggtta aggagctcgc ggccgagatc
    11221 gaagcggcag aacagccgct ggtgacagtc gacctggaaa cgcaagccat cacgatgccg
    11281 tccggccgcc aagtcacatt cacgaccgat cccgagcgcc gcgctgcttt gttggaaggc
    11341 ctcgacgaga tcggtatgac actcaagctc gatgcgcaga tccgcgggca tcaaagccgg
    11401 gaccgcgaga gcaggccctg gatttaccga cgagaggaga ctgcgcgcgt ggcacgttta
    11461 ttgatcctgg ccggtgacgg catcggtccc gaaattcttg ccgaggtgcg gaaagtcgcc
    11521 gaatggttca tcgccaagcg gcaacttagc gtcgagctgc gtgaggagct gttcggcatt
    11581 tccgcgtgga aaacgcatgg cgatttgatg cgccagcaga cctgggacga gatcgtcgcc
    11641 gccgacgcca tcttgttcgg cgctaccgga tcgccggaat acgagaaaat ccctgccgct
    11701 gcgcgcaaag tcgatcagct tctccggatg cgcaaggaac tcgacctgtt ctgcaattta
    11761 cgtccggtgc gcacgcttcc ggcgcttggg gcctcttcaa gcttgcgtcc cgaggtcatt
    11821 gcagattgcg atatgatgat cgtgcgcgag ctgtgcggcg gcatctattt cggcaagccg
    11881 cgtggccgcg aaaagctcaa caccggcgga gagcgcgccg tgaacacctg tatgtacacg
    11941 acaggtgaaa tagaacggat cgcccgcgcc gccttcgatc tcgcgcgtgt ccgctcaggc
    12001 cgcgtatgct cggtcgataa gtccaacgtg ctgaccgaga ctggcggcct gtggcgcgat
    12061 actgtgcagg cgctgcaaga tcggcattat cccgacatcg agctcagtca catgtatgtc
    12121 gacaactgca ccatgcagct ggtacgcgca ccgaagcaat tcgacgtttt agtgactgaa
    12181 aaccttttcg gtgacatctt atcggattgc gccgccatgg ttgcagggtc gctggggatg
    12241 ttgccgtcag cgtcgctggg cccagtacgc cccgacggcg gccgtcacgc tctctatgag
    12301 ccgatccacg gcagcgcccc cgacatcgcc ggcaggggca ttgccaatcc gctgggtgcg
    12361 attatgagct ttgccttgtg ccttcggttt tcgctgggat tggcacagga agcggatcgc
    12421 ttggagcgtg ctgtcgacgc cgcgatctcc catggagcac gtacagcgga cattatgcag
    12481 cccggagaaa aaagtatctc gagcaaggcc atgggcgatg ccgtgcttgc ggagttggag
    12541 caaatgcaat gatggcgctt cgagttggaa accccagtag acgaccgccg aggccgctac
    12601 tggcgcggag tctgcatttt ggaagtggac attgaccaat tgttgcccaa aattatgatc
    12661 agaaattcgt cccgcggaaa cgggtgcaac acatttgcaa cattcgggcg taagccattg
    12721 ataaatcgat agtcaaaacg gtttcctaaa ccgaaggtcg gaggttcgac tcctctcggg
    12781 accaccaaac ctactttgaa tttccaagtg ttttaagacg aattgtcaaa ccgggactgc
    12841 aatgtctgct ttcgggggta aagcggacat gggctagtgc actgcaaatg tccgcatatg
    12901 acccgaagcg gacattgggt gtgcatcgcg gcaacgattt tgatgccgat ttcagcccct
    12961 atcaatttac tcgtttactt gcctgtcttt ttgcgttcaa caagcgaaac gaaaatccaa
    13021 cacaaaatcg caatcgcaac gcctgcggcg ataatctccg cccaatagat gtcgctctga
    13081 gacatgtatt tcttttaggt attgcgcccg tcgatgtcgc ataattattg cgtacaaaaa
    13141 gaagcctcgg cgcgcgaagc tgcgcctgaa cctaattgac tgtacgttta cgagaaagtt
    13201 atttgtaagt gcgctgctag tagcgttgat tgctccagtg cgcgctgctg ttttcttcca
    13261 gagggaaggc agcatgaatc ctccacgacc actgcacaaa cccgcgccct aaccggcgcg
    13321 gtttttttta tgtgatagga atgcaaagat agtcgcggct actctcacca gttcccttaa
    13381 aaatccgcta ttggacaaca acgcgcaatc caaccgttgc cgcgtttgtc cgctactgac
    13441 taaagcggac aaaggaccgc ccgggcgccg aatagctccg cagcgaatga tgtggtggac
    13501 ggcgcctgct cccggcatcg aggtgccata gggtggttcg ttggagacga atcacatgag
    13561 aggagccgtc cacatgcaag ttaccacgat cggtttggat ctcgccaa
//
LOCUS       DATMGX010000596         2910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_75495, whole genome shotgun sequence.
ACCESSION   DATMGX010000596 DATMGX010000000
VERSION     DATMGX010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75495"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VNR65_09760"
     CDS             <1..949
                     /locus_tag="VNR65_09760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HWJ18997.1"
                     /translation="SFLAQGAQMCLVLAIAPAVTGITRRVKARLTRRRGAPILQGYRD
                     LLKLVRKDVVLAENASWYFRSAPYLIFAFTWVAAALVPTLASGLMFNWAADVVALVAL
                     IGAARALLALAAMDVGTAFGGIGSSREMMIASLAEPATLLVALTIAFAAGTTRLPEVA
                     QYLINQPVELRVSFALAFVALVIVALAENARIPVDNPATHLELTMVHEAMVLEYSGRH
                     LAMIEAAGYLKLVLYISLIICLFLPFGMAPIDGGPAAWAVGLAAYLAKVSIAAVVLGV
                     WEVSIAKMRVFRVSEFLGGAFVFSFLAILLAFLSKEVLR"
     gene            946..1602
                     /locus_tag="VNR65_09765"
     CDS             946..1602
                     /locus_tag="VNR65_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ18998.1"
                     /translation="MTNAYDVAHFLAGMMLVCSFTLLYQDRIFAVLNTFAAQAMMLSL
                     AVAWQGYVQSSPDLFITAAMAFGLKGIVIPVALNRMIIRLGIQRDVDQVVNAGVAMFL
                     GLGLTSLALLLVLKVTPGAASFTREGLALALAIILLGFLMMIIRRNAVTQIVGFMSLE
                     NGLILAATGARGMPLVVEVSVAFSVLIALFVFAVFVFRIRERFDSVDIEALERVRGAR
                     "
     gene            1602..>2910
                     /locus_tag="VNR65_09770"
     CDS             1602..>2910
                     /locus_tag="VNR65_09770"
                     /inference="COORDINATES: protein motif:HMM:NF005043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HWJ18999.1"
                     /translation="MNQAVYAVVGIPFLSAILLALITSYRVTAIGNVLASGLTFAAGL
                     SFLALPPVVKNYVILDDLNVFFIILNTLVGFTTSVFSRSYIEHEIETGHLTPRFVRYY
                     HAMFQAMMGGMNLALLANNIGLMWVGLEFATLMTVIMVGIYRTPTALEAAWKYFILGI
                     VGISLAFFGTIIVYLAGQSVLGEGVAAMTWSRLMEVAPQLDAPLISLAFIFLLVGYGT
                     KVGLAPLHAWLPDAHAEGPTPVSAVLSGLLLNVALFALLRFKMLLAAHPGALKPGPIM
                     ITIGLISLIFAAFMLYQRRDIKRLFAYSSIEHMGLITFAFGMGGPLANFAGLLHMVMH
                     SLTKSAIFFSVGHVAQAKGTQKLADIRGLSVSHPALAVGLAAGVIAISGLPPFGAFMS
                     EFLILTSTFAREPLLALAVAFGLLVAFGALLLRLQNVLFGAPSE"
BASE COUNT          450 a          896 c          818 g          746 t
ORIGIN      
        1 atcctttctc gcccagggcg ctcaaatgtg tttggtgctc gccattgcac ccgcggtgac
       61 agggatcacc cggcgcgtca aggcaaggct gacgcgacga cggggagcgc ccatccttca
      121 aggctaccgc gacctcttga agctcgttcg caaagacgtc gtgctcgccg aaaacgcttc
      181 ctggtacttc cgctcggcgc cttacctgat cttcgctttt acctgggtgg cagcggccct
      241 cgttccgacg ctggcgtcgg ggctgatgtt caattgggcc gccgatgtgg tcgcattggt
      301 tgcgctcatc ggcgctgctc gggcgctgct tgcgcttgcg gccatggatg ttggcacggc
      361 ctttggcgga atcggctcat cgcgcgagat gatgatcgcc tcgctcgcgg aaccggcgac
      421 gcttctcgtg gcgctaacga ttgcgttcgc cgccggcacg acaaggctgc cggaagtcgc
      481 gcaatatctg atcaatcagc ctgtcgaact ccgggtttcc tttgcgcttg cttttgttgc
      541 gctcgtcatt gtggcgcttg ccgagaatgc tcgcattccc gtcgacaacc ccgccacgca
      601 tcttgaattg acgatggtgc acgaggccat ggtgctcgaa tattcgggcc gccacctcgc
      661 catgatcgaa gcggcgggtt atctgaaact cgtgctctac atttccttga tcatttgcct
      721 cttcctgccc ttcggaatgg cccctatcga tggcggccct gcagcctggg cggtcgggct
      781 cgccgcatat ctggcgaagg tctctatcgc agccgtggta ctcggtgttt gggaagtgag
      841 catcgcaaaa atgcgggtat tccgggttag cgagtttctc ggcggcgcct tcgttttctc
      901 cttcctggcg atcttgctcg cgttcctttc aaaggaggtg ctccggtgac gaacgcctac
      961 gacgttgcac acttcctcgc cgggatgatg ctcgtttgca gcttcacgct tctttatcaa
     1021 gaccggatct tcgccgttct caacacgttc gcggcgcagg ccatgatgct gtcgctcgct
     1081 gttgcgtggc agggctatgt tcagagttcg cccgatctct tcatcaccgc ggccatggcc
     1141 tttggcctca aggggatcgt tatacccgtc gccttgaacc gcatgattat ccgcctcgga
     1201 attcaacgcg atgtcgatca ggtggttaat gcgggagtag caatgtttct cgggcttggg
     1261 ctcacaagcc tcgcgctgct gctcgttctc aaggtgacgc ctggggcagc atccttcacg
     1321 agggaaggtc ttgcactcgc ccttgcgatt attcttctcg gcttcctcat gatgataata
     1381 aggcgcaacg ccgtcaccca gatcgttggc ttcatgtctc tcgagaacgg ccttattctc
     1441 gccgcgaccg gcgcgcgggg catgcctctc gtggttgagg tcagcgtggc tttctccgtg
     1501 ctgattgccc ttttcgtttt tgcagttttc gtcttccgca tacgggagcg ctttgacagc
     1561 gtcgacattg aggccctcga gcgtgtccgg ggagcacggt aatgaaccaa gctgtttatg
     1621 cagtcgtcgg catcccgttt ctatcggcga tcctgcttgc tcttataacg agctacagag
     1681 tgacggctat cggcaacgtg ctcgcctccg ggctaacctt tgccgcgggc ctctccttcc
     1741 tcgcccttcc tcctgtcgtt aagaattacg tcatcctcga cgatctcaat gtcttcttca
     1801 tcattctgaa cacgcttgtc ggtttcacca cgtctgtgtt cagccggagc tacatcgagc
     1861 acgaaatcga gacgggacac ctgacgcccc gcttcgtccg gtattaccac gctatgttcc
     1921 aggccatgat gggtgggatg aacctcgcgc ttctcgccaa caatattggc ctgatgtggg
     1981 tgggtctgga attcgcaacg ctgatgaccg ttatcatggt cgggatctac aggaccccga
     2041 ccgcgctcga ggcggcctgg aagtatttta tccttggcat cgtcggcatt tcgctcgcat
     2101 ttttcggtac gatcatcgtt tatctcgcgg gacagagcgt tctgggggag ggcgtcgctg
     2161 cgatgacctg gagccggctt atggaagttg cgccgcaact cgacgcgccg ctcataagcc
     2221 ttgcgtttat ctttctgctt gtcggctacg gcacgaaggt gggccttgcg cccttgcatg
     2281 cttggcttcc cgatgcccat gctgaaggcc cgacccccgt ttcggcggtg ctatcgggcc
     2341 tgctcctgaa tgtggcgctc tttgcgcttc ttcgcttcaa aatgctgctc gctgcgcatc
     2401 cgggcgcgct caagcccggc ccgatcatga tcaccatcgg gctgatctca ctgatcttcg
     2461 cggcgttcat gctgtatcag cgccgcgaca taaaacgctt attcgcatac tcctcgatcg
     2521 agcacatggg actcatcacc ttcgcattcg gcatgggcgg tccgctcgcg aatttcgcag
     2581 gtctcttgca catggtgatg cacagcctca cgaaatccgc gattttcttt tccgtcggcc
     2641 atgtcgcgca ggcgaaaggc actcagaagc ttgccgatat ccggggcctg agtgtctcgc
     2701 atcccgcgct tgccgtgggt ttggccgccg gcgtaattgc aatttccgga ttgccgcctt
     2761 tcggcgcgtt catgagcgaa tttcttatcc tcacgtccac cttcgcccgg gaaccgctgc
     2821 ttgcgcttgc ggtagctttc gggctgctgg tcgcgttcgg cgccctcctt ctccgccttc
     2881 agaatgtcct tttcggcgca ccttccgaac
//
LOCUS       DATMGX010000597         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_288160, whole genome shotgun sequence.
ACCESSION   DATMGX010000597 DATMGX010000000
VERSION     DATMGX010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288160"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VNR65_09775"
     CDS             complement(<1..631)
                     /locus_tag="VNR65_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HWJ19000.1"
                     /translation="MLYQAYQFYSDATVPARKIAELGLGAMVPFTGEANGAWLRKFTA
                     ALELVSRSGLTHTRPAYGITSVRVGNRDVAVTEEALATTPFGTLLHFKKDIEQAQPRV
                     LVVAPLSGHFATLLRSTVQTLLADHDVCITDWHNARDAPLSAGTFGFEDYIDHLIQWL
                     RVIGPGGHVLAVCQPCVQVLAAVAVMAEAGDPAKPKTMTLMAGPIDTRVN"
     gene            704..1384
                     /locus_tag="VNR65_09780"
     CDS             704..1384
                     /locus_tag="VNR65_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWJ19001.1"
                     /translation="MNHKRGLWSMAEAKLTISSKNYSSWSLRGWLLCKLADLDIEEEV
                     VSIDDPSIRAELLLLSPSFLVPCLSHNGIKIWDTLAIAEYLAEQNPRSQLLPKELKAR
                     SHCRSVSGEVHSGFYNLRSALPMNLRAHHEGFQVFPGARADIEHIFSIWRDCLQTYGG
                     PYLFGPLTMADAMYAPVCTRFLTYDLKLDQVCTDYCRSIMVWPAMEQWVADAKAEPEE
                     LEELDVEF"
     gene            1690..2676
                     /locus_tag="VNR65_09785"
     CDS             1690..2676
                     /locus_tag="VNR65_09785"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ19002.1"
                     /translation="MTMNPALSAFNHVVAFEVAKDSLVIHVLPADKQSVAANKPRAIR
                     KALLAEMRRNAECGLGPLLAVCEATGGYERHVLAICVELGIAVHKAHGARVRHFARYL
                     GLLAKTDPIDARVLALYGLKTQDLRLYVPLTPEVIALRDLKNRRDQVQQMLFAETNRL
                     EHARHASVSRSLKAHIASLRKTLAALEAEIAAHVKACETLARKARLMRTLKGVGPATV
                     AAILAYMPELGAFTKGEAACIAGLAPINNDSGTFQGSRHIEAGRAAVRKTLYMTALVA
                     IQHNPVMRPFADKLRRSGKPSKVVIAAVMRKLIVTLNAILRSGEPWRHAKAA"
     gene            complement(2885..>3207)
                     /locus_tag="VNR65_09790"
     CDS             complement(2885..>3207)
                     /locus_tag="VNR65_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWJ19003.1"
                     /translation="RKATGGTIAFEIMGTSDAVWTDPDDLWVQDVASVVQSVTGGASK
                     IGGASYFTDAAALKPGMGNPPTIILGPGEPSMAHQTDEWCSIKGIEGAEAIYSELVAR
                     WCGL"
BASE COUNT          633 a         1008 c          923 g          643 t
ORIGIN      
        1 ggttgacgcg cgtgtcgatc ggccccgcca tcagcgtcat ggtcttcggc ttggccggat
       61 cgcccgcttc cgccatcacg gccacggctg cgagaacctg gacgcaaggc tggcagaccg
      121 ccaacacatg cccgcccggc cctatgacgc gcagccattg gatgaggtgg tcgatataat
      181 cctcgaagcc gaacgtgcct gcggagagcg gtgcgtcgcg ggcattgtgc caatcggtaa
      241 tgcagacgtc gtgatcggcg agcaaggtct gcaccgtgct gcgcagcaag gtcgcgaagt
      301 ggccggagag tggagccacc accagcacgc gcggctgcgc ctgctcgata tccttcttga
      361 aatggagcag cgtcccgaac ggtgttgtgg caagcgcctc ctcggtcacc gcgacatcgc
      421 ggtttccgac gcgcaccgag gtgatgccat aggcggggcg cgtgtgtgtc agccccgagc
      481 gcgacacgag ttccagggcc gccgtgaact tcctcagcca cgccccgttg gcctctccgg
      541 taaatggcac cattgcgcca agccccagct ccgcaatttt tcgtgccggc accgtggcat
      601 cagagtagaa ttgataggct tgatagagca ttcttagcct gagcgctgga acggatgttg
      661 ggtgggccgc cgtgcccgga catcgtagct ggcgatgcaa atagtgaacc acaagcgagg
      721 gttatggtcc atggcggagg cgaagctaac cataagcagt aagaactatt cctcgtggtc
      781 gctgcggggc tggcttttgt gcaagctggc cgacctcgac atcgaggaag aggttgtctc
      841 catcgacgat ccttcgatac gcgccgagct gcttttgctg tccccgtcgt ttctggttcc
      901 ctgtctctct cacaatggca tcaaaatttg ggacacgctc gccattgccg aatatctcgc
      961 tgagcagaac cctaggtcgc agctccttcc caaggagtta aaggcgcgat cgcattgccg
     1021 ctccgtctcg ggcgaggtgc attccggctt ttacaatctg cgctcggcgc tgccgatgaa
     1081 ccttcgcgcc caccacgagg gctttcaggt gtttccaggc gcgcgagccg atatcgagca
     1141 catcttttcg atctggcggg actgcttgca gacctatggc ggcccctacc ttttcggccc
     1201 gcttaccatg gccgacgcca tgtatgctcc ggtgtgcacg cgttttttga cctacgatct
     1261 gaagctcgat caggtctgca ccgattattg ccggtccatc atggtctggc cggcgatgga
     1321 gcagtgggtc gcggacgcga aggcggagcc ggaggaactc gaagagcttg acgtcgagtt
     1381 ctgatcgccg ccgccgtcat cccggaaggg cggagctcta tccgggatcg taacacattg
     1441 acatgcttat tttttgcgat cccggcttgt atcttgggac ggctgacctt tctatcgcca
     1501 acgaatcgga aagcatcagc ccgtgatgag ggtggccacc ctcatcacgg gcggcgcgcg
     1561 gtcgtatccc ggatagacca tacacggtcc agccctatct ttgacccgcg ccgccgagtt
     1621 ccatgttcat ccgaacggta gaagggcctt gcacggccgc atacaatcca ggatcacgga
     1681 ctctccatca tgactatgaa cccggcttta tctgcattca accatgtcgt cgcgttcgaa
     1741 gtcgccaaag acagtttggt tattcatgtt ctacccgccg ataaacagag cgttgcagcg
     1801 aacaagccga gggcgatccg caaagcgctg ctcgccgaaa tgcggcgcaa cgccgagtgc
     1861 gggcttggcc cgctcctcgc cgtctgcgag gcgaccggcg gctacgagcg ccatgtcctt
     1921 gcgatctgcg tggaacttgg aatcgccgtg cacaaagccc acggcgcacg ggttcgccat
     1981 ttcgccaggt atctcggcct tctcgctaaa accgatccga tcgacgcccg cgttctcgcc
     2041 ctctatgggc tcaagaccca ggatcttcgc ctttacgtcc cgctaacccc tgaggttatc
     2101 gcactcagag atttgaaaaa ccggcgtgat caggttcagc agatgctctt cgccgaaacc
     2161 aaccggctgg agcatgcccg ccacgcgagc gtcagccgaa gccttaaagc gcatatcgcc
     2221 agccttcgca aaaccttggc agcgcttgag gccgagatcg cggcccatgt caaagcctgc
     2281 gagacgctcg cgcgcaaagc gcgcctcatg cgaaccctca agggcgtcgg ccctgcgacc
     2341 gtcgcggcca tcctggctta catgcccgaa ctgggcgcat tcacaaaggg cgaggccgcc
     2401 tgtatcgcgg gcctcgcgcc aatcaacaac gatagcggca cgttccaagg ctctcgccat
     2461 atcgaggcag ggcgagctgc tgtccgcaag acgctttaca tgacggcgct cgtcgccatc
     2521 caacataacc ccgtcatgcg gcccttcgcc gataaactca gacggagcgg caagccgagc
     2581 aaagtcgtta tcgccgccgt catgcgaaaa ctcatcgtaa ccttgaacgc catactccga
     2641 tccggcgaac cttggcgcca tgcgaaagcc gcttgacgcc taagacggta gatagggctc
     2701 cgcccttccg ggatgacgcc cgcggcgatc cgttgacgcg cccgaaagac tttgctcagg
     2761 ttccggcgcc tttgccgatc gccgttctta ggtagagaca aaacgtactt atatgcttac
     2821 ttatagctga tctacaaaac gaaaccaatt ttaactggct gtaaactttg aaggcaggaa
     2881 aggctcagag cccgcaccaa cgggcgacga gctcggagta gatggcctcg gctccttcga
     2941 tcccttttat ggagcaccac tcatcggtct ggtgcgccat ggaaggttcg ccaggaccca
     3001 atatgatcgt cgggggattg cccattccag gcttcagcgc cgcggcgtcg gtgaagtagc
     3061 ttgcgccgcc gatcttggaa gcgcctcccg taacggattg tacaacgctc gcaacatctt
     3121 ggacccaaag atcgtcggga tccgtccata cggcgtcgga agtgcccatg atctcgaacg
     3181 cgatagtgcc gcccgtcgct ttcctga
//
LOCUS       DATMGX010000598         2947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_244410, whole genome shotgun sequence.
ACCESSION   DATMGX010000598 DATMGX010000000
VERSION     DATMGX010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244410"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VNR65_09795"
     CDS             <1..504
                     /locus_tag="VNR65_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19004.1"
                     /translation="YHDALELEKRSSEPCSMPEIDPVVEELAPHPVPGPEAPAVVDVI
                     EAASAREVAEATHDFGIPAAVAEPVPDYAAPHEEPAPPAEPPVEFKPEPVVAVEPAPE
                     PAPAPEPAAAPVIVTKAEPPRAAPVPESPPVTAVKKTDATPKQPQKEPEKTFFGLWLD
                     MVFGRRK"
     gene            663..2354
                     /locus_tag="VNR65_09800"
     CDS             663..2354
                     /locus_tag="VNR65_09800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ19005.1"
                     /translation="MSLAADMSHATSSLLAPDLLRLCDEAAENAERLVRRAVVAVRAK
                     VSAGDALSSELMEREQHACHGLAWLATYCEGIKQMASYARRLNEAHRLGEIEALLTQI
                     GIGEYAAQVLGGIPMSQSEFIRSFDLGLEAKDREMFASEAVVTLIKANSPKARTRLVE
                     LVQHAAGTGHFGDGGLDDTLEAMRGEIIRFVDAEVKPYAHEWHMKNEYIPLETIAKMN
                     ELGVFGLTIPEEFGGLGLGKEAMCVVSEELSRGYIGVGSLGTRSEIAAELILGGGTPE
                     QKEHWLPKIASGEVLPTAVFTEPNTGSDLASLKMRGVREGDVYKITGQKTWITHPVRA
                     DLMTVLARTNSAEPGYRGLSMFLAEKPRGTDENPFPAQGMTGGEIEVLGYRGMKEYEL
                     SFDGFEVPANGLLGGEEGQGFKQLMQTFESARIQTAARAVGVAQSAMDIGLRYAMDRQ
                     QFGKAIINFPRISDKIAMMAAETMIARQLTYYAGREKDQGHRCDLEAGMAKLLAARVA
                     WSSADNALQIHGGNGFALEYPISRVLCDARILSIFEGAAEIQAQVIARRLLEVKS"
     gene            2503..>2947
                     /locus_tag="VNR65_09805"
     CDS             2503..>2947
                     /locus_tag="VNR65_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ19006.1"
                     /translation="MMDDVRGRTALITGASAGIGKAFAEVFAAKGWNVVLTARRLERL
                     EALAEELTKKHKVAVTPIAGDLAQPDGVMRLIEAISANRLIIDGLVNNAGYGVPGYYA
                     RTRWEDQEAFLRVMVTAPCELAHVLLTPMVQGRFGRIINVASLAGL"
BASE COUNT          583 a          888 c          965 g          511 t
ORIGIN      
        1 taccatgatg cgctggaatt ggagaaacgc agttccgaac cgtgctctat gccggaaatc
       61 gatccggtcg tcgaagagtt ggcgccgcac cccgttccgg gccccgaggc gcccgctgtg
      121 gtggatgtga tcgaagcagc atctgcacgc gaagtggccg aggcgacgca cgatttcgga
      181 atcccggctg cggtagcgga gccggtgccg gactatgccg cgcctcacga ggaacccgcg
      241 cctccggcgg agccccccgt agaatttaag ccggagccgg tggttgccgt tgaaccggca
      301 cccgaacccg ctccggcacc ggaaccggca gctgcgcccg tgattgtgac caaggccgag
      361 ccgccgcgcg ccgcacccgt gccagagtcg ccgccggtta cagccgtcaa aaagaccgat
      421 gccactccaa aacagccgca aaaagagccg gagaagacgt tcttcggtct gtggctcgac
      481 atggtgttcg gccgaaggaa ataactccgg ccctttggag gcgctccttg acgatgagcg
      541 cctccaaagc cggaaattgc tatagacgcg aacgaagcgg cggcaatccg gcatttcgaa
      601 acccggttgt ggcatgctac gctcgggaga atttaaagaa tcggacgaaa ggaaaggcaa
      661 gcatgagcct cgccgccgac atgagccacg ccacctcttc cctcctggct cccgatctgc
      721 ttcggctctg cgacgaggcc gcggaaaacg ccgaaaggct tgtccggcgt gccgttgtgg
      781 ccgtgcgggc caaggtcagc gcgggcgacg ctctcagcag cgagttgatg gagcgcgagc
      841 aacacgcctg ccacggcctc gcctggctcg ccacctattg cgagggcatc aagcagatgg
      901 cgtcctacgc gcggcgcctc aacgaagctc atcgcttggg cgagattgaa gcccttctca
      961 cgcagatcgg cattggcgag tacgcggcgc aagttctcgg cggcatccct atgagccagt
     1021 ccgagttcat acgctctttc gatctcggtc ttgaggccaa agaccgagag atgtttgcga
     1081 gcgaggcagt tgtcacgctc atcaaggcca atagcccgaa ggcacgcacg cgcctcgtcg
     1141 aactggtgca gcacgcggcg ggcacaggcc atttcggcga tggcggcctc gacgacacgc
     1201 tggaggcgat gcgcggcgag atcatccgct tcgtggacgc cgaggtgaag ccgtatgccc
     1261 atgagtggca tatgaagaac gagtacatcc cgctcgaaac catcgcgaag atgaacgagc
     1321 tgggagtatt cggcctgacc atcccggagg agtttggcgg gcttggcctt ggcaaagagg
     1381 cgatgtgcgt cgtatccgag gaattgagcc ggggttacat cggggtcggc tcgctcggca
     1441 cccgctcgga aatcgcagcc gagcttattc ttggcggtgg cacgcctgag cagaaggagc
     1501 actggctccc gaagatcgcg agcggcgaag tgctgccgac ggcggtcttt accgagccga
     1561 atacggggtc ggacctggcg tcgctcaaga tgcgcggcgt gcgcgagggc gacgtctaca
     1621 agatcaccgg ccagaaaacc tggatcaccc atcccgtgcg agcggacctg atgacggtgc
     1681 tggcgcggac taattctgca gagccgggct atcgcgggct ttccatgttc ctggcagaga
     1741 agccgcgtgg cacggacgaa aatccattcc ctgcgcaggg catgacgggc ggcgagatcg
     1801 aggtgctcgg ctatcgcggc atgaaggagt acgagctgtc gttcgacggg tttgaagtgc
     1861 ccgcgaacgg gcttctcggc ggcgaggaag ggcagggctt caagcagctc atgcagacct
     1921 tcgaatcggc gcgcatccag acggcggcgc gagccgtcgg cgttgcccag tccgcgatgg
     1981 atatcgggtt gcgctatgcc atggatcgcc agcagttcgg caaagcgatc atcaatttcc
     2041 cgcgcatctc cgacaagatc gccatgatgg ccgccgaaac catgatcgcc cgccaactca
     2101 cctattatgc aggccgggag aaggatcaag gccatcgctg cgacctggaa gcgggcatgg
     2161 ccaagctgct ggcggcgcgc gtggcgtggt cgtcggcgga caatgcgctg caaatccacg
     2221 gcggcaacgg ctttgcgctc gaatatccca tcagccgcgt gctctgcgac gcgcgcattt
     2281 taagcatttt cgagggcgca gcggaaatcc aggcgcaggt catcgcgcgc cgtctcctcg
     2341 aagtgaaaag ctaggcttac ttactccctc tcccacgggg agagggtttg gggtgaaagg
     2401 cgagatttta tctcttcacg ctctcccccc tcacccgaac gcgtggcggt ccggcagcga
     2461 atgccgccca gagcgtttgc tatttaagcc cattaggaga gcatgatgga tgatgtgcgc
     2521 ggccgcactg ctttgatcac tggcgcgtcc gcgggcatcg gcaaggcctt tgccgaagtc
     2581 tttgcggcca agggctggaa cgtggtgctc acagcgcgaa ggctggagcg gctggaagcg
     2641 ctggccgagg agcttaccaa gaagcacaag gtcgcggtca cgcccatcgc aggcgatctc
     2701 gcgcagccgg acggagtaat gcggctcatc gaggcgatca gcgccaacag gctgatcatc
     2761 gatggcctcg tcaacaacgc cggatatggc gttcccggat attatgcgcg cacaagatgg
     2821 gaggatcagg aagccttcct gcgcgttatg gtgaccgcgc cgtgcgagct tgcgcatgtg
     2881 ctgctgacgc caatggttca gggccgtttt gggcgcatca tcaatgttgc ctcgctcgcg
     2941 gggctgg
//
LOCUS       DATMGX010000599         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_297184, whole genome shotgun sequence.
ACCESSION   DATMGX010000599 DATMGX010000000
VERSION     DATMGX010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297184"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /gene="purH"
                     /locus_tag="VNR65_09810"
     CDS             <1..1550
                     /gene="purH"
                     /locus_tag="VNR65_09810"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWJ19007.1"
                     /translation="SVFDKTGIVDFARRLAGLKIEILSTGGTAKLLREEGVAVKDVSE
                     FTSWPEMLGGRVKTLHPKIHGGLLFRRSNAEDEKQAREHGIAPIDLVVVNLYPFEATA
                     AKGYLPAEELIENIDIGGPTMLRSAAKNFESVTVITDPADYDRVAKELEANRGTSLQL
                     RLRLARKVFGATSRYDGVITAELERLIEQNGKLEVAPREKLPERLYLSLRRKQELRYG
                     ENPHQAAALYIPAGRPAEGLAAAKQLQGKELSYNNYVDLEAARSLVAEFEKPAAVIIK
                     HNNPCGAAQQATLVEAYIQAFACDPVSAFGGVLAFNHIVDAVTAEEVVKLFVECIVAP
                     GFADRAQEIFSAKKNLRLLQLPQGGLHHEKELQLKRILGGMLVQQPDLGTLKGKELKV
                     VTKREPSESELADLKFAWKICKHVKSNAIVFAKDGATMGVGAGQMSRVDSVKIAVLKA
                     QKSLQGTAVASDAFFPFPDGVEEAAKAGATCVIQPGGSVKDPEVIAAADRLGLAMVFT
                     SMRHFLH"
     gene            1699..>2245
                     /locus_tag="VNR65_09815"
     CDS             1699..>2245
                     /locus_tag="VNR65_09815"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ19008.1"
                     /translation="MKPRRICLAISLAALTALPLLAQTSFRDTAKPPEKMSPRAEAYY
                     NFALGHLYEIQFEQTSQSEYATRSIEAYKKAYSIDPQSPIIGERLAEMYWKAQRVHDA
                     VTEANEILKRDPNNLATHRLLGRIYLRSLGDINNGSSGVQNEMVAKAIAEFSEVHRLD
                     PADMESALWLARLFRLHNQPEK"
BASE COUNT          491 a          679 c          621 g          454 t
ORIGIN      
        1 tcagcgtatt cgataaaacc ggaatcgtgg atttcgcgcg gcgtctcgcg gggctgaaga
       61 ttgaaatcct gtctaccggc ggcaccgcga agttacttcg cgaagaaggt gtggcggtca
      121 aggatgtctc ggaatttacc agttggccag aaatgcttgg cgggcgcgtc aagaccctgc
      181 atcccaaaat tcatggcggc cttttgttcc gccgcagtaa cgcggaggat gagaaacaag
      241 ccagggaaca cggcatcgcg cccatcgatc tggtcgtcgt taatctttat cccttcgaag
      301 ccacggcggc caagggctat ctgcccgcgg aagaattgat cgaaaacatc gatatcggcg
      361 gtcccaccat gctgcgttcc gcggcgaaga attttgagag cgtcacggtg attaccgacc
      421 ccgccgacta cgatcgcgtg gccaaggaac tcgaagcaaa ccgcggcacc agtttgcaac
      481 tccgtttgcg actggcgcgc aaggtgttcg gcgcaacctc ccgctacgac ggtgtgatca
      541 ccgccgaact ggaacgactt atcgaacaga atggcaagct cgaggtggct ccgcgggaaa
      601 agcttcccga gcgtttgtac ttgtcacttc gccgcaaaca agaattgcgt tacggcgaaa
      661 atccccatca agccgccgcc ctctacatcc ccgcaggtcg tcccgcggag ggtcttgctg
      721 ccgccaagca gctccagggc aaggaactct cctacaacaa ttacgtcgac ttggaagccg
      781 cgcgcagcct ggttgccgaa tttgaaaagc ccgccgccgt catcatcaag cacaacaatc
      841 cctgcggagc ggcgcagcag gccaccttgg tggaagccta tatccaggcg tttgcttgcg
      901 atcccgtctc ggccttcggt ggagtcctgg ccttcaatca catcgtggac gcggtgaccg
      961 ccgaggaagt cgtcaaactt tttgtggaat gcatcgtcgc gccgggattt gcggaccgcg
     1021 cccaggaaat tttctcggcc aagaaaaatc tgcggctgct gcaattgccg caaggcggat
     1081 tgcatcacga aaaagagctg cagctgaaac gcatcctcgg cggcatgctg gtgcagcaac
     1141 ccgacctcgg cacactaaaa ggcaaagagc tgaaagtggt gacgaaacgc gagcccagcg
     1201 aatccgagct ggccgacctg aaatttgcgt ggaagatctg caagcacgtg aagtccaacg
     1261 ccatcgtctt tgccaaagat ggcgcaacga tgggcgtcgg tgcgggacag atgagccgcg
     1321 tggactccgt caaaattgcc gtgctgaagg cccagaagtc gctgcagggc acggccgttg
     1381 cctccgatgc tttcttcccg tttccagatg gtgtggaaga ggccgccaag gcaggcgcca
     1441 cctgcgtcat ccaacccggt ggctccgtaa aagatcccga agtgattgcc gccgccgatc
     1501 gcctgggcct cgctatggtg tttaccagca tgaggcattt tctacattga gttttagcgg
     1561 caattgcgcc gtttttggcc catttgccgc tcggcagcat ctcaattctt gagccccgta
     1621 agcgtatcgc acgcatctgt tttttcgctt ttgaccgcct ttcccgctca tgggataatc
     1681 ttagtttatg gactccttat gaagccacgc cgtatctgcc ttgcgatttc cctggctgct
     1741 ctgacagccc tccctctgct cgcccagact tccttccgtg acaccgccaa gccaccggaa
     1801 aaaatgtcgc cccgcgccga ggcctactac aatttcgcgc tgggccatct ctacgagatt
     1861 cagttcgagc agaccagcca gagcgaatac gcgacgcggt ccatcgaagc ctacaagaag
     1921 gcctattcca tcgatccgca gtcgcccatc atcggagagc gcctggccga aatgtattgg
     1981 aaggcccagc gggttcacga cgcggttact gaagccaacg aaattctgaa gcgcgacccc
     2041 aataatctgg ccactcaccg gcttctcggc cgcatctatc tgcgcagcct cggcgacatc
     2101 aacaacggca gctccggcgt gcagaacgaa atggttgcca aggccattgc cgaattcagt
     2161 gaggttcacc gtctagaccc ggcggacatg gaatcggcgt tatggcttgc gcgcctgttt
     2221 cgcttacaca atcagcccga gaagg
//
LOCUS       DATMGX010000600         2291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_197027, whole genome shotgun sequence.
ACCESSION   DATMGX010000600 DATMGX010000000
VERSION     DATMGX010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197027"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VNR65_09820"
     CDS             complement(<1..1017)
                     /locus_tag="VNR65_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWJ19009.1"
                     /translation="MRYRPIALICLATLLGTSRLCAQQQPAQNPPAPAQPSPAKPETP
                     QQSGTTVVVKVNLVDVLFTVLNRRNKLVPDLEKQDFKIFDDNIPQDIRYFSKQTDLPL
                     RIGMLLDTSNSIRDRLKFEQDAANNFLFSVLRHGKDEAFLMTFDDEPQVIQGFTPDTG
                     KLRDQILRTRAGGGTAVYDAIYTACEKELSHPPRPPGDQPDVVRRVMILISDGEDNLS
                     THTRAEAIEMAQRTSVVIYTISTSTQWITLDDPSKEKSGNRKYHLTEGDKILQDLAEE
                     TGGRAFYPYHVDDLDQSFQDIGDELRNQYSIAYNPTNHAYDGRYHKIKIEMPDHKGYQ
                     ARARR"
     gene            complement(1027..2031)
                     /locus_tag="VNR65_09825"
     CDS             complement(1027..2031)
                     /locus_tag="VNR65_09825"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWJ19010.1"
                     /translation="MSEVQTIHLTITDADAGLRLDRVVAAHCAELSRTRVQELIELGL
                     VLLNGKPAKDSQKLRAGDVIQVDAQPRPPLQAEPESIPLNVLYEDDDVIAINKSAGMT
                     VHAGAGNAHGTLVNALLGRGLDLSKSDDPLRPGIVHRLDKETSGVLLVAKNDFAHAKL
                     ADSFRDRTIQKTYIALVQGLLTEDRGRIELPIARHPNHRTRMTADRAGLLPNSRPART
                     DWKVCARIGPTTLLEIHLHTGRTHQIRVHFSALHHPVVGDTLYGADTQLHVAHTTLPP
                     LERNFLHAAKIVFAQPRSGKPITLTAPLPQELRHFLHALATASGEDPTRIDDALAGFL
                     "
BASE COUNT          443 a          484 c          856 g          508 t
ORIGIN      
        1 gcgtctggcg cgggcttggt agcccttatg gtcgggcatt tcgattttaa ttttgtggta
       61 gcggccatcg taggcgtgat tggtagggtt gtaggcgatg gagtattgat tgcgcagttc
      121 gtcgccaatg tcctggaaag actgatccaa atcatcgacg tgatagggat aaaacgcacg
      181 gccgccggtt tcctcggcca ggtcctggag aatcttgtcg ccttcggtga ggtgatattt
      241 gcggttgccg gatttttcct tgctggggtc gtcgagagtg atccactgag tgctggtgct
      301 gatggtgtag atgaccacgc tggtgcgctg ggccatttca atagcctcgg cgcgagtgtg
      361 agtggagagg ttgtcttcgc catcgctgat gaggatcatg acgcggcgca cgacgtcggg
      421 ctggtcaccg gggggacgcg ggggatggct tagttctttt tcgcaagcgg tgtagatggc
      481 gtcgtacacg gccgtgccgc cgcccgcgcg ggtacgcaga atctgatcgc ggagcttgcc
      541 ggtatcggga gtaaagccct ggatgacttg cggttcgtcg tcaaaggtca taaggaaagc
      601 ttcgtctttg ccgtggcgga gaacgctgaa gaggaaattg ttggcggcat cctgttcgaa
      661 tttcagccgg tcgcgaatac tgttggaggt atcgagcaac atcccgatgc ggagaggaag
      721 atcggtttgc ttgctgaaat agcggatgtc ttggggaata ttgtcgtcga aaatcttgaa
      781 atcttgtttt tcgagatcgg ggacaagttt gttgcggcga ttgaggacgg taaatagcac
      841 gtcaacgaga ttcactttaa cgacgacagt ggtgccgctt tgctgaggag tttccggctt
      901 ggctggagaa ggttgagcgg gagcgggcgg gttctgtgca ggttgttgct gggcacaaag
      961 ccgcgaggtt cctaggagag ttgcgaggca gatgagagca atagggcggt agcgcatgcc
     1021 ggatgattat aggaagcccg caagggcatc gtcaattcgg gtggggtcct caccggaggc
     1081 ggtggccagt gcgtgcaaga aatggcgaag ttcctggggc aggggggcag tgagggtgat
     1141 gggcttgccg gagcgaggtt gcgcgaaaac gattttggcg gcgtggagga agttgcgctc
     1201 gaggggtggc agggtggtgt gtgcgacgtg gagttgcgtg tcggcaccat agagagtgtc
     1261 gcccacgacg ggatggtgca gcgcggaaaa gtgcacgcga atctggtgag tgcgtccggt
     1321 gtggagatgg atttccagca gcgtggttgg gccgatgcgg gcgcaaactt tccaatcggt
     1381 gcgggcggga cgggagttgg ggagcagccc ggcgcggtcg gcagtcatgc gggtacggtg
     1441 gttgggatgg cgcgcgatgg gaagttcgat gcggccgcgg tcttcggtga gcagtccttg
     1501 aacaagagcg atataggttt tctggatggt gcgatcgcgg aagctgtcgg ctagcttggc
     1561 gtgagcgaaa tcatttttgg cgaccagaag cacgccggag gtctccttat cgaggcggtg
     1621 gacgatgcct ggacggaggg ggtcgtcgga tttggagaga tcgaggccgc gtccgagcaa
     1681 ggcgttgacc agcgtgccgt gggcatttcc ggcaccggcg tgaacggtca tgccagcgga
     1741 cttgttgatg gcgatgacgt cgtcatcttc atataggaca ttgaggggga tggattcggg
     1801 ttcggcctgg agaggaggac gcggttgggc atcgacttga atgacatcgc cggcgcgaag
     1861 tttttgtgag tcttttgcgg gcttgccgtt cagaagcacc aggcccagtt cgataagctc
     1921 ctggacacga gtacgggaga gctcggcgca atgggcagcg acaacgcgat ccaggcgtag
     1981 accggcgtcg gcatcggtga tggtgaggtg gatggtttgg acttcggaca cctagagagt
     2041 gtagcgcggg ataagggcga atcgaaattt tcgtgattgc gctcgagaag gagcaattga
     2101 tagcggaata ggaaaagatg gaagggccgc agcgctgact ggcgagtcaa agctcggcgg
     2161 cggcgaaaag aaaagttatt cgaccaggga ccaggagtcg tcggggtcga aggcggtggt
     2221 ggacttggcg atgtccgcgg tttccgggcc tagatctttt tcgtagatgc cgccgtcctg
     2281 attgacgatg a
//
LOCUS       DATMGX010000601         2257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_17050, whole genome shotgun sequence.
ACCESSION   DATMGX010000601 DATMGX010000000
VERSION     DATMGX010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17050"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VNR65_09830"
     CDS             <1..444
                     /locus_tag="VNR65_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19011.1"
                     /translation="APGAQPKSSHIGPAEIYPDSMMTPGAANIDVTQQNIQDNICSRR
                     WSTKQIRPPSEYTSKLKRKQLRQYGDTVHQTRAELINPNTGKLDTTRCVAHSDPNPPI
                     TEERLGFRITQQGICVEKTPLGGMRRAMRVGVSGRKPPQVVQGQG"
     gene            complement(520..1953)
                     /locus_tag="VNR65_09835"
     CDS             complement(520..1953)
                     /locus_tag="VNR65_09835"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HWJ19012.1"
                     /translation="MRKLTKPSPTASPLLFELDPEPLQETLTALGGIPLVVQAFRSLG
                     LPGSIQEHVRVKQRQRGYDDATFVESFVILNAAGGECVEDFERLRQDPGLAEMIGHAV
                     PSPRAALEFLQAFHQEEKIEEAQQRRLPEQIAYIPEETSALEGLGRVNRDLVQRFGER
                     VPDQRIATVDQDATIIESHKQQALTTYEGVRGYQPMLAAWAETGLVLADQFRDGNVPA
                     QMEPLEVARRAFAALPGTVQEYYYRGDAACHESGLVNWLRDEQRAGGPCGRIGFAISA
                     RMSEPLHAALEALPEAAWETYGDPHPAEIRECADIPFVPSEKTEKKDLQPLRYVAIRI
                     RHKQGGLFEDGSAVRQFAVLSNRWELKPARLIEWHREKAGTIELVHDVIKNDLGGGVL
                     PSKYFGANAAWLRLAVIAHNVLQALKRLALPAELLTARPKRLRFLLFNMPGRLVHHAR
                     KLILRVAAAGKWTGTYGDGLRLLPVIT"
BASE COUNT          448 a          724 c          662 g          423 t
ORIGIN      
        1 gcgccggggg ctcagccaaa gtccagccac attggacccg cggaaattta tcctgactct
       61 atgatgactc ccggcgctgc aaacattgat gttacgcagc agaacatcca agacaacatc
      121 tgctcccgcc gctggagcac aaagcaaatt cggccgccat cggagtacac cagcaagctg
      181 aaaaggaaac agcttcggca atacggagac actgtccatc agacaagggc ggaactcatc
      241 aatcccaaca cggggaagct cgataccact cggtgcgtgg cgcactctga ccccaatccg
      301 ccgataacgg aggagcgttt gggatttagg ataacgcagc agggaatctg cgttgagaaa
      361 actccgctcg gtggaatgcg ccgtgccatg agggttggag taagcggccg gaagccgccg
      421 caggttgtgc agggccaagg atgaagacac tgcgaacgga gttttgaaaa ggtggtttac
      481 aaatccgcgg ataagcacga ccttttggtg caggtaggtt caggtgatca cgggcagcaa
      541 gcgcaggccg tcgccatagg tgcccgtcca cttgcccgcg gcggccactc gcaggattag
      601 ttttcgggca tggtgaacca gccgccccgg catattgaag agcaggaagc gcaagcgctt
      661 ggggcgcgcc gtgagcagtt cggcgggcag ggccagccgc ttcagtgcct ggagcacgtt
      721 gtgtgcgatc accgccaggc gcagccacgc ggcgttggct ccgaagtatt tcgagggcag
      781 cactcccccg cccaagtcgt tcttgatcac gtcatgcacc agctcgatgg tgccggcctt
      841 ttcgcggtgc cactcgatca agcgcgccgg ctttaattcc caccggttcg acaacaccgc
      901 gaactggcgc accgcgctcc cgtcctcgaa cagtccgcct tgcttatggc gaatgcggat
      961 ggctacatag cggaggggtt gcaaatcttt cttctcggtt ttctcgctgg gcacaaaggg
     1021 gatatccgcg cactcgcgga tttcggccgg gtgcggatcg ccgtaggtct cccaggcggc
     1081 ttcaggaagg gcctcaaggg cggcgtgcaa gggctcgctc atccgcgcac tgatggcaaa
     1141 gccgatgcgc ccgcacggtc cgccggcccg ctgctcatcg cgcaaccaat tcaccagccc
     1201 gctttcgtga cacgccgcat cgccgcggta gtaatactcc tgcacggtgc ccggcagggc
     1261 ggcaaaggcc cgtcgcgcca cttccagcgg ctccatctgg gcgggcacgt tgccatcgcg
     1321 aaattgatcg gccagcacca gcccggtctc cgcccacgcc gccagcatgg gctgatagcc
     1381 tcgcacccct tcgtaagtgg tcaaggcctg ctgcttgtgg ctctcaatga tcgtggcatc
     1441 ctgatccacc gtggcgatgc gctggtcggg cacgcgctcc ccgaagcgct gcaccagatc
     1501 gcggttcact cttcccaacc cctccagcgc ggacgtctct tccgggatat aggcgatctg
     1561 ctccggtaag cgccgttgct gggcttcctc gatcttctcc tcctggtgaa acgcctgcaa
     1621 aaactccagc gccgcgcgcg gcgaaggcac cgcgtgccca atcatctccg ccagcccggg
     1681 gtcttgccgc aggcgctcaa aatcttccac acattcgccc cccgccgcat tgagaatcac
     1741 gaagctctcc acgaacgtcg cgtcgtcgta accgcgctgc cgctgtttca cccgcacgtg
     1801 ctcctggata ctcccgggca gccccagcga gcggaaggcc tgcaccacca gcgggattcc
     1861 tcccagcgcg gtcagggttt cttgcagcgg ctcgggatca agctcgaaca acaagggcga
     1921 ggcggtgggc gaaggcttgg tgagtttgcg cataggggta agaacccagc aattttcaca
     1981 cccaacgggt gaaaactctt agaacattcc cggacttccc cccaactccc accgcccttg
     2041 tttacggccc gcgcccacac tgcgccccag ctcagcctgg acaagccgcc ccttatcggc
     2101 ggatcgggga ttgtctggag gcctttaatt tccctgggtg tatgatagcc aaatgaaaag
     2161 gactgcttcg cgggaacgcg gttactccta ggagaataac catgagagtc tattacatca
     2221 tcattggaat ggctgtggtc gtgattttgc tgcttgg
//
LOCUS       DATMGX010000602         2598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_124809, whole genome shotgun sequence.
ACCESSION   DATMGX010000602 DATMGX010000000
VERSION     DATMGX010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124809"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..2138)
                     /locus_tag="VNR65_09840"
     CDS             complement(111..2138)
                     /locus_tag="VNR65_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWJ19013.1"
                     /translation="MRPDPIELEVFKHLFHSIAEEMGAALRRTAFSPNIKERRDYSCA
                     LFDAAGNVLAMGDHMPVHLGSMPMSVRAAIAELPLAPGDVAMLNDPFRGGTHLPDITV
                     VAPVFVRSAQHKMRRARRDADPEFFVAARAHHADVGGTYAGSMGPCREIYQEGFRIPP
                     VKIVRGGTLDRDILALLLNNVRTPEEREGDLSAQLAACHTGSARLLELCARYGIARVQ
                     RAAPALLDYSEKMMRAFLATVPPGCYAAEDVLDDDGVTDEPVRIAVRITFPRATKVAS
                     RNARPVIIDFTGSSPQVEGSINAVEAITYSACFYVFRCLLREDVPATAGLMRPIQLIA
                     PEGTIVNARPPAAVAGGNVETSQRIVDVLLRALAQALPEHIPAASSGTMNNLTIGGMD
                     DRHLGVPHSSHPERSASGVEGPAAFARAGRSDVRSSVILSDKSGRHGDRTRSRRTPIL
                     PTNLPLARFSTERSASGVEGPAALARAGRSDVRWSVILSDESGRHDDRTRSRRTPIPP
                     TTLPLTRSYIERSASAVPAVPFAYYETIAGGSGASAAHDGVSGVHTHMTNSLNTPAEA
                     LEYSYPLRVMKYALRSGSGGEGKHRGGDGIVREIEVLADATVTMLSDRRKSRPYGLAG
                     GGDGAPGRTEIFRADGSIELMPGKGSTRLRRGERVRIETPGGGGWGKVEQD"
BASE COUNT          495 a          773 c          831 g          499 t
ORIGIN      
        1 gctcagggca gggtctaaag gtgcgcccct tcaaaatcgg tctgccgatc acgctgaacc
       61 attttcgctc ttttccgttc ggctgcggct ccaaggaaac gcgcaagatg tcagtcctgt
      121 tccactttgc cccagccgcc gccgccgggg gtttcgatgc gaactcgttc gccgcgccgc
      181 aagcgcgtgc tgcccttgcc gggcatgagt tcgatggaac catcggcgcg aaatatctcg
      241 gtgcgtcccg gcgctccgtc gccgccgccg gcgagaccgt agggtcgaga cttgcgccgg
      301 tcggagagca tggtcacggt ggcgtcggcg agcacttcta tttctcgcac gatgccgtcg
      361 ccgccgcgat gttttccttc cccgccgcta cccgaacgca gcgcatactt catcaccctt
      421 aaagggtagc tgtattccag cgcttcggcg ggcgtgttga gcgaattggt catgtgggtg
      481 tgcacgcccg agacgccgtc gtgggccgcg ctggctcccg agccgccggc gatggtttcg
      541 tagtacgcga agggaacggc tggtaccgcg cttgcgctcc gctctatgta agaacgtgtt
      601 aagggaaggg ttgtaggtgg tataggggtc cttcgactcc tcgtccggtc gtcatggcga
      661 ccggactcgt cgctcaggat gacagaccaa cgaacgtcag agcgccccgc cctagccaaa
      721 gcggcaggtc cctcgactcc gcttgcgctc cgctccgtcg aaaaacgtgc caagggaagg
      781 ttagtaggca gtataggggt ccttcgactc ctcgtccggt cgccatgacg accggacttg
      841 tcgctcagga tgacagacga acgaacgtca gagcgccccg ccctagcgaa agcagccggt
      901 ccctcgactc cgcttgcgct ccgctcggga tgactagaat ggggcacccc caaatgacga
      961 tcatccatgc cgccgatggt gagattgttc atggtcccgg acgatgccgc ggggatgtgc
     1021 tcgggcagcg cctgggccag tgcgcgcaga agtacgtcaa cgatgcgctg cgaggtctcg
     1081 acatttccgc ccgccacggc tgcgggggga cgcgcattga cgatggtgcc ctcgggcgca
     1141 atcaactgaa tgggccgcat caatccggcg gtcgcgggaa cgtcttcgcg cagcaggcag
     1201 cggaaaacgt agaagcaggc ggaatacgtg atggcttcca cggcgttgat gctgccctcg
     1261 acttggggcg agctgccggt gaaatcgatg atgacgggtc gcgcattccg gctcgcgact
     1321 tttgttgcgc gcgggaacgt gatgcgcacg gcaatgcgca caggttcgtc ggtgacgcca
     1381 tcgtcatcaa gcacgtcctc ggcggcgtag catcccggag gcaccgtcgc caagaatgcg
     1441 cgcatcatct tctcggagta atccagcagc gctggcgcgg ctcgctgaac tcgcgcgatg
     1501 ccgtaacgcg cgcaaagttc gagcagtcgc gcgctgccgg tgtgacacgc cgccagttgc
     1561 gcgctcaggt cgccttcacg ttcttcggga gtgcgaacat tgttcagcaa cagggcaagg
     1621 atgtcgcggt cgagagttcc gccgcgcacg atcttcaccg gcggaatgcg aaatccttcc
     1681 tggtagatct cgcgacacgg gcccatcgaa ccagcgtagg ttccaccaac atcggcgtga
     1741 tgcgcgcgag ccgcgacgaa gaattcggga tcggcgtcgc gcctggcgcg tcgcatcttg
     1801 tgctgggcgc ttcgcacgaa caccggagcg acgacagtga tgtcgggcag atgcgtgcct
     1861 ccgcggaagg ggtcgttgag catcgccaca tcgccgggcg cgagcggcag ctccgcaatg
     1921 gcggcgcgca ccgacatggg catcgagccg agatgcacgg gcatgtgatc gcccatggca
     1981 agcacgttgc cggctgcgtc aaagagcgcg caggagtagt cgcgacgctc tttgatgttg
     2041 ggcgagaagg ccgtgcggcg cagcgctgcg cccatctctt ccgcgatgga atggaagaga
     2101 tgcttgaaga cttcgagctc gatcgggtcg ggccgcatga cggagcgagt gtacctcaga
     2161 ggctggcgaa ggggagattt tgtcctgtcg cggtgggagc gaatcaccaa attccaaagc
     2221 gcgcaataga tttttaaaaa tatttgttga cattccgctc cagtaaccgc aatatctggg
     2281 gttagacata ctgctcgtgc aacatcttgg gcgggtatgg cattttgggt caaaaaaatc
     2341 aaccttcgag ggagaaggtt acacaaggag gaatacccaa tggcaactaa gaagaaggcc
     2401 acgaagaaag ctgctaagaa gaaaaagcgg taactgtttc tggcgaggca ctggcctcgc
     2461 ttaacttttg ccaataaact gggcctccgc aaattgcagt tcgcggaggc ctttcccttt
     2521 gcgcttcccc tcactgtggc ggattcggcg agctctccag cgtctcatcg caagtgccat
     2581 tggccggttg cgtcggct
//
LOCUS       DATMGX010000603         2105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_415356, whole genome shotgun sequence.
ACCESSION   DATMGX010000603 DATMGX010000000
VERSION     DATMGX010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415356"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VNR65_09845"
     CDS             <1..185
                     /locus_tag="VNR65_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19014.1"
                     /translation="TSRTDPEQRDRARDIYEENIRQSSIHSSKAIWQSVRDRRSEAIE
                     WESGCIRDPTNNATTR"
     gene            202..654
                     /locus_tag="VNR65_09850"
     CDS             202..654
                     /locus_tag="VNR65_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19015.1"
                     /translation="MSLADQNWLAVALAYLIQIDESRRAENAYGSSYCIFEAGRAYFQ
                     CLAPSFGTYLRCEAVSEKFVPEMGAILTPEKRDRLVQEFGFTAPGYSKNFSQKIEIKD
                     KDDLAYVARMAFRVLRDVYAVTDFGATKFKVLLPEPAKPVSVQIRDHR"
     gene            713..1384
                     /locus_tag="VNR65_09855"
     CDS             713..1384
                     /locus_tag="VNR65_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HWJ19016.1"
                     /translation="MCGRFTQSYTWRELYELYRLTCQPVNIEPRYNIAPTTMINVVVP
                     RETGRDLPTMRWGLIPSWWKKTAKEAPPTFNARAETVATKPMFRTAFKQRRCLIPASG
                     YYEWKATPTGKQPYYLSASDGSVLSFAGLWDEWRDIETGEAVKSCTIIVTDANALTRQ
                     IHDRMPVVIAPEQFEPWLTGEAGTELLKSAPEEMLQMWPVSRKVNRVGNDSDPTLIDL
                     IRYSA"
     gene            1396..1677
                     /locus_tag="VNR65_09860"
     CDS             1396..1677
                     /locus_tag="VNR65_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19017.1"
                     /translation="MSWYQGFFDPIILPGRKPLVTLRDAAHYIMSLPQAEQQAPEWQA
                     AIEVLMLIGENGGDPMMAHIGMMRALHRHKPKAAPAPRRKRAKAYKIIR"
     gene            complement(1841..>2105)
                     /locus_tag="VNR65_09865"
     CDS             complement(1841..>2105)
                     /locus_tag="VNR65_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19018.1"
                     /translation="IDGSNQIGYLSAQFAEIILKNCGDELTRENLLKQATNLSGISLP
                     LLLPGITVSVSPDDYSTFSTFRLAKFDGKTWKFFGENISTASK"
BASE COUNT          490 a          572 c          598 g          445 t
ORIGIN      
        1 caaccagccg aaccgacccc gagcagcgag accgtgcgcg agatatttat gaggaaaata
       61 tccggcaatc ctcgattcat tccagcaaag ccatctggca aagcgttcgt gatcgtcgga
      121 gcgaggccat cgagtgggaa agcggctgca ttcgagaccc tactaacaac gctacgacac
      181 gatgagccag ccctcggcag cttgtcgctt gcggatcaga actggcttgc agttgcgctc
      241 gcttatttga tccagatcga tgaaagccgc agggctgaaa atgcttacgg gagctcgtat
      301 tgtatttttg aggctggtcg cgcctacttc caatgcttag caccttcatt tggcacatat
      361 ctgcgatgtg aagcggtttc agaaaagttc gttcccgaaa tgggggcgat cctcacgccg
      421 gaaaagaggg atcggttggt gcaggaattt gggttcactg cgccgggcta ttccaagaat
      481 ttctctcaga agatcgagat taaggacaaa gacgatcttg cttacgttgc tcgaatggcg
      541 tttcgggtgc tgagggatgt atatgccgta acagacttcg gtgcgacaaa gtttaaggtg
      601 ttgctcccag aacctgcgaa gccggtttcc gtacaaattc gtgatcacag ataattacga
      661 cgtcattggc gatatccacg gactttaatt ttcacaagac cagcatcgat caatgtgcgg
      721 acgcttcacc caatcctaca cttggcgcga gctctatgag ctttaccggc tcacttgcca
      781 gccggtaaac attgagccac gctacaacat cgcgccgacc acaatgatca acgtggtggt
      841 gccgcgtgag actggccgtg atctgccaac gatgcgctgg ggcctgatcc cgtcatggtg
      901 gaagaagacg gctaaagaag cgccgccgac cttcaatgcc cgcgccgaga cggtcgccac
      961 gaagccgatg tttcgcaccg ccttcaagca aaggagatgc ctcattccgg cctcgggcta
     1021 ttacgagtgg aaggccaccc cgaccggcaa gcagccctat tacctttccg cgagcgatgg
     1081 ctcggtgctg tccttcgccg ggctgtggga tgaatggcga gacatcgaga ccggcgaagc
     1141 ggtgaagtcc tgcaccatca tcgtcaccga tgccaacgct ttgacccggc aaattcacga
     1201 ccgcatgccg gtagtgatag cgcctgagca attcgaaccg tggctaaccg gtgaggcagg
     1261 cacagaattg ctcaagtccg cgccggaaga gatgctgcag atgtggccgg tttcccggaa
     1321 ggtgaatcgg gtcggcaatg acagcgaccc gactttgatc gatttaatcc gatacagcgc
     1381 ttaatggagt tgggcttgtc ctggtatcaa gggtttttcg atccgatcat attgcccggc
     1441 cgtaagccgc tggtcacgct tcgcgacgcc gcgcactata ttatgagcct gccccaggct
     1501 gaacaacaag cgccggaatg gcaggccgcc atcgaggtgc tgatgctgat cggcgagaac
     1561 ggtggcgacc cgatgatggc gcacatcggc atgatgcgag cgctgcacag gcataagccc
     1621 aaggcggcac cagcaccgcg ccggaagcgc gcgaaagcct ataagatcat tcgatagcca
     1681 agagggcacc aacggcggtc ctacttgttc gcttctccca gcagcttgcc ggtcaagcta
     1741 ttgacgaaaa gcagcttggc gcaggtgccg gaaaatacac agagttcagc tccgcgagga
     1801 aacgcccgac tgccgatcga ggcaaccggg cgtcgtttgt ttatttcgaa gcggtgctga
     1861 tgttttcacc aaagaacttc caggttttcc cgtcgaattt cgccaaccgg aatgtgctga
     1921 acgttgaata gtcatccgga gacaccgaaa ccgtgatgcc cggaagcaga agaggtaatg
     1981 aaattccgct caggttggtt gcctgcttga gaaggttctc gcgagtgagc tcgtcgccgc
     2041 agtttttcag gataatctcg gcgaattgag ccgataggta gccgatctga ttgcttccat
     2101 cgatc
//
LOCUS       DATMGX010000604         4368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_160836, whole genome shotgun sequence.
ACCESSION   DATMGX010000604 DATMGX010000000
VERSION     DATMGX010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160836"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /locus_tag="VNR65_09870"
     CDS             <1..1867
                     /locus_tag="VNR65_09870"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HWJ19019.1"
                     /translation="ASIEEHIDARKTTDWTGPELTLPHWQPSAVLPDSQETLFSPRST
                     PLQIEKERPWSSQSPALPIELSAGNEMELTLPEITQAFHLLEMDAGEGSSLEVSYVLP
                     EGQQSGRCTYIARSGVQTWMGGDTFAFVKLRLRVTIGRIRLTRASAYEVRYPFERAAS
                     FTCSDPFLSQLWGICARSLELLSEDAYVDCADRERVEWTDDSPPAFDCTRVMMRGPDG
                     RGRAYWGDARLLGSLLRRIALTQQPDGQLKAHSCSERFDIHAIMEDRSCDWVVLLREY
                     LESSGDTELVRELWPTLTRLLDWYRKRRTDRGLVLAREWEVWDNPLRYQVCEGAGLNA
                     MVYRALVDASVLATSIGKNHDAKTLAQEAERLQGDYNHLLWNEQEGAYDGALFGPGSQ
                     IRSQLGSKFSGPIVAGRFRPAAQANLFALYSGIVPPQHLTSVRKWVLNHLEEVREPMS
                     HYYLFRMLYAMDDEQQDTEVLRLMRTEWKNQVESEWQTAWEELEKGGGSKVHIYGMHP
                     GYFLTAYVLGARREGPVDLRTILIEPRFSGLDWARGVCVTEFGPVEMEWKMNHARRPE
                     IVCTVPKKVRAKLRLRSREENDLLKVDGIDVYSRSSNGWLEIMLQPGKHTVQFRS"
     gene            2352..3479
                     /locus_tag="VNR65_09875"
     CDS             2352..3479
                     /locus_tag="VNR65_09875"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HWJ19020.1"
                     /translation="MIKLNGSVSLRVAAACVATALSTAAGADLTVYIGTHTAAPGKGF
                     SVAHFDTVTGALSKPEFLLETPAPAYFVIAPGGRRLYSCNSTGFVSAYSIDPATARLA
                     LINQEPSGGGDPSYISLDRTGHYVFVANYDGGNIAVWAIQPDGSLGDRTAFVQHTGSS
                     VDPRRQTHAYAHSIVVDPTNRFVLVADLGLDKLFVYRFNVKDGSLSPNDPPFARSAPG
                     SGARHVVFHPNGRWVYLITEMGNTIILFNWDTKNGTLSEVQTTPTLPKDFQGVSTCAE
                     VRVHPSGRFVYASNRGHDSIAVFSVDAHTGRLTPIQHVPSGGKTPRNFEMDPTARWML
                     VTNHGNEKPMVFRIDQETGKLTPVGQPVDVPAPFCPRFLAR"
     gene            complement(3519..>4368)
                     /locus_tag="VNR65_09880"
     CDS             complement(3519..>4368)
                     /locus_tag="VNR65_09880"
                     /inference="COORDINATES: protein motif:HMM:NF037199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HWJ19021.1"
                     /translation="VNRVAEAGKREFPLPMFLNVWTDIQDGFYDPGFSHPSGGATTRM
                     LPLYKAIVPAIDWISPDVYKPSYVQFIEEATPYSRPDNPLFIPETGHDLGFCRRMFYA
                     LGDLKGIGVSVFGVEGSADGNNSSVPEELRELEATYRVVSAATPLLADAKHFGKLRSM
                     VEEQGITTLVADFGEFEAMAQFGPLHWGYGGARAGGTPRTSGRALVGQTGPQEFVVAG
                     FDATVNFRPRFGSAAPRADFVSAEEGAYEAGVWKPRRQLSGDEIFFGLRLPAEGTVVK
                     VKLMKY"
BASE COUNT          856 a         1379 c         1327 g          806 t
ORIGIN      
        1 ggcgtcgatc gaagagcaca tcgacgcccg caaaaccacc gactggaccg ggcccgagct
       61 gactcttccc cactggcagc cgtcggcagt tctccccgat agtcaggaga cgctattttc
      121 cccacgcagc actccgctcc aaatcgagaa ggagcggcca tggtcctcgc agtcgcccgc
      181 attgcccatc gagctctccg cgggcaacga gatggaactc accctccctg agatcacgca
      241 ggcgtttcac ttgttggaga tggacgccgg agaaggtagc tcgctagagg tctcctacgt
      301 cttgcccgag gggcaacaga gcgggcgatg cacctacatc gctcgctccg gcgtgcagac
      361 ctggatgggt ggcgacacct ttgccttcgt gaagcttcgc ctgcgcgtga ctattgggcg
      421 gattcgcctg acgcgcgcgt cggcctacga ggtgcggtat ccctttgagc gggccgcgtc
      481 gtttacatgc agcgatcctt ttctcagtca gctatggggt atctgcgctc gctcgctaga
      541 gctgttgagc gaggacgcct acgtcgattg cgcggaccgg gaacgtgtcg agtggacgga
      601 cgatagccct ccggccttcg attgcacgcg cgtgatgatg cgcggccccg atggccgggg
      661 tagagcgtac tggggtgacg cccgcctgct gggcagtctg ctgcggcgca tcgcactcac
      721 ccagcagccg gacggccagc tcaaagctca tagctgctcg gaacgattcg acatacacgc
      781 cattatggaa gaccggagct gtgattgggt ggtgctgctg cgtgagtatc tcgagagttc
      841 cggcgacaca gagctggtgc gcgagctatg gcctacgctg acccggctgc tcgattggta
      901 ccgcaagcgg cgcaccgatc gtggcctggt cctcgcgcgg gagtgggaag tctgggacaa
      961 tcccttgcgc taccaggtct gcgagggcgc aggcttgaat gcaatggtct atcgggccct
     1021 cgtcgatgct tccgtgctgg ccacgagcat cggcaaaaac cacgacgcca aaactcttgc
     1081 tcaggaggcg gagcgtctgc agggcgacta caaccatctt ctttggaacg agcaggaagg
     1141 ggcctacgac ggtgctctct tcggacccgg ctcccaaata cgttctcagt tgggcagtaa
     1201 gttctccggc cccatcgtcg ccggtcgctt tcgccccgca gctcaggcaa acctcttcgc
     1261 gctctacagc ggtattgttc cacctcaaca ccttacctcg gtgcggaagt gggttctgaa
     1321 ccatctggaa gaagtgcgcg aaccgatgtc gcactattac ctcttccgaa tgctgtacgc
     1381 aatggatgac gagcaacagg acactgaagt cctgcggctc atgagaacgg agtggaaaaa
     1441 tcaagtcgag tccgaatggc agacggcctg ggaagagctg gaaaaaggag gagggtccaa
     1501 agtccatatc tacggcatgc accccggcta ttttctgacg gcctatgtat tgggggcacg
     1561 aagagagggt ccggtggatc tgcgaacgat tttgattgag ccgaggttct cgggcctcga
     1621 ttgggcaagg ggcgtatgcg ttaccgagtt cggcccggtc gaaatggaat ggaagatgaa
     1681 tcacgctcgc agacctgaga tcgtctgcac cgtgccgaaa aaggtaagag ctaagctccg
     1741 gcttcgctcc cgtgaggaaa atgatcttct gaaggtcgac ggaatcgacg tatattctcg
     1801 cagctcaaac ggctggttgg aaatcatgct gcaaccgggc aaacacacag ttcagtttcg
     1861 gtcctgatgt tgcccgcgaa gagccgttgc ggcaacgctg ccgatcttgc ccaagcgagc
     1921 tgtgccgaac caagagatcg tgcatctggc cggcagtagt ttgcgcaaaa tcgccaacga
     1981 tcaaaccgac tgctcgcaaa cagcagccct ggggattaat cccggcaaac ccgcttgaga
     2041 gaacgcatcg gagctctctg ggtggtttgt cggcaacgca caaggggtct ctggcgtttc
     2101 gattcggccg ctgatcccct cccgcacggg gggccggcgg taactcggaa gcgatcaggc
     2161 aagtgcagcc gtggcgggat tcgttgcagc cgcggcgcga cccgtttccg gaccccctac
     2221 gatcgggagg gcgctctcga tccgggacgc gcacgaagcc accaccaggg caaccaaact
     2281 gtccttcacg ccggccgaac gcaccgtgca ccggagtaag ctggtaaggt tcggagaagg
     2341 agtcagtcca gatgatcaag ctcaatgggt ctgtttcgtt aagagtagcg gccgcctgcg
     2401 ttgccacggc gctctccacc gcggcagggg cagacctgac ggtctatatc ggcacccaca
     2461 ccgcggcgcc cggcaagggc ttttcggtcg ctcatttcga taccgtcacg ggcgcgctga
     2521 gtaaacccga gttcctcttg gaaactcctg ccccggccta tttcgtgatc gcgccgggcg
     2581 gccgccgcct ctattcctgc aactcgacgg gttttgtcag cgcctattcc atcgatccgg
     2641 ccaccgcgcg cctggcgctc atcaaccaag agccgagcgg cggcggcgac cccagttaca
     2701 tcagcctgga caggaccggt cactatgtct tcgtagccaa ttacgatggc ggcaatattg
     2761 ccgtttgggc gattcaaccc gatggcagcc tgggcgaccg caccgccttc gtgcaacaca
     2821 cgggcagcag cgtggacccg cggcgtcaaa ctcacgcgta cgcgcattcc atcgtggtgg
     2881 accccaccaa ccgatttgtc ctggtggccg acctgggcct ggacaagcta ttcgtttacc
     2941 ggttcaacgt gaaggacgga tcgctttcgc ccaacgatcc gccgttcgcc aggtcggctc
     3001 cgggctcggg cgcgcgtcac gttgtgtttc atccgaacgg ccgctgggtt tacctgatca
     3061 cggaaatggg aaataccatc atcctgttca attgggacac gaagaacggc actctgtccg
     3121 aagtacagac cacgccgacg ctacccaagg acttccaggg cgtcagcacc tgcgccgagg
     3181 tgcgggtgca tccgagcggc cggttcgtgt acgcttcgaa tcgcgggcac gacagcatcg
     3241 cggtcttttc cgtcgacgct cacaccggcc ggctcacgcc catccagcac gttccctctg
     3301 gcggcaaaac gccgcgcaat ttcgagatgg accccaccgc gcgctggatg ctcgtcacca
     3361 accacggcaa cgaaaaaccg atggtgtttc gcatcgacca ggagaccgga aaactgacgc
     3421 cggtgggaca accggtcgac gtccctgccc ccttctgccc gcgcttcctg gcgcggtaac
     3481 cggcggcgga ggactgcccg ccgccggagg ccgcacgatc agtatttcat cagcttgacc
     3541 ttgacgacgg ttccctccgc cggcaatcgc aagccgaaaa agatttcatc gccactcaac
     3601 tgccggcgcg gcttccacac gcccgcctca taagcgccct cctcggcgct gacaaaatcg
     3661 gcgcggggcg cggccgagcc aaacctggga cggaagttca ccgtcgcgtc gaaccccgcc
     3721 accacgaact cctgcggccc ggtctgccct accagcgcgc gtccgctggt ccgcggcgta
     3781 cctccggcac gggcgccgcc gtagccccag tgcaaggggc caaactgcgc catagcctcg
     3841 aactcgccaa aatcggcaac cagcgttgtg atgccctgct cctccaccat ggatcgcagc
     3901 ttgccaaaat gcttggcatc cgccagcagc ggcgtggcgg cgctgaccac gcgataggtg
     3961 gcctcgagtt cgcgcagttc ttctggaacc gaagaattgt tcccgtcggc ggaaccctcc
     4021 acgccgaaca ccgagacacc gatgcccttc aggtcgccaa gagcgtagaa catgcgccgg
     4081 cagaaaccca ggtcgtggcc ggtttctgga atgaacaggg ggttgtccgg acggctgtag
     4141 ggtgtggcct cttcgatgaa ctgcacatag ctcggcttgt agacatcggg agagatccag
     4201 tcgatggccg gcacaatggc tttgtacagc ggaagcatac gggtggtcgc tccgccgcta
     4261 gggtgcgaaa agcccgggtc gtaaaagccg tcctgaatgt cggtccagac gttcaggaac
     4321 atgggcagcg ggaactcccg cttgccggct tcggccacgc ggttgacg
//
LOCUS       DATMGX010000605         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_109822, whole genome shotgun sequence.
ACCESSION   DATMGX010000605 DATMGX010000000
VERSION     DATMGX010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109822"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..>2127
                     /locus_tag="VNR65_09885"
     CDS             164..>2127
                     /locus_tag="VNR65_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWJ19022.1"
                     /translation="MNTSDTAKSNDIITAKNVDLSTCDRELVQYPEAIQPHGVMLTVD
                     DHTNLIVHASANCAQLLGGGPDAIIGKSADNVLGPTGRDLIGKLRRMALDGGPVHVAR
                     ESFVRSDRGFHLFAHRSGGLIILELESAPPSAQPLPNLYSELRADIARLQETKSLAAF
                     FDLAVEKIRKFTGFDRVMAYRFDEDGSGHVVAESKRDDLEAYLGLHYPATDIPSPARR
                     LFSLAWVRHLPDVDYVPVSLVSAKTYTSGPVDMSFASIRSVSVMYSDYLKNMGVKATM
                     VMPLMKEGKLWGLISAMHHAAPRHVPHETRMAAEFLAHTLSLLMSAKEDTEVFERVLA
                     MSETTDRLIQALSVEPDFGKTLGTPETLADVLNQVEAAGAAVVSQSKVSLTGKTPPEE
                     AVRDLAQWIGNEGGPLFATDRLPSLYPPAEAFARAASGVLAVRISPDRPEFLLWFRPE
                     QIEVVTWAGDPHKPVDVSEADGSVRLRPRGSFALWKESVRNRSTPWHENEKTAALKLR
                     VGIADLVLGGAGKIDRISRELEASHADLHSFTHAASHDLKDHLHGIHHLASTLKRRQG
                     DVLDEEGRQQIATILKLTQRMDGLIDALLDHARVDKTAMALESVNLDEVVDSALLGLG
                     HSLAEGGVDVRRPSRLGTALCNRERIRDVLV"
BASE COUNT          394 a          662 c          656 g          415 t
ORIGIN      
        1 agctcaagcg gctccggttg ccaaagacgg tcgcaggtgt caaatttgct agcggatcga
       61 gatcgtgtcg cccatccggc cgcgcgtcat tgccctggcc tgatccataa ctccgtcagc
      121 aggaccggtg acgcctcgcg tgcgaggtca gggaacgatc atcatgaata caagcgacac
      181 cgccaaaagc aacgacatca tcacggcaaa gaatgtcgat ctctcgacgt gcgaccgtga
      241 gttggttcag tatccggagg ccatccagcc gcatggcgtc atgctcacgg tggacgacca
      301 caccaatctc attgtgcatg ccagtgcgaa ttgcgcgcag cttttgggcg gcgggccgga
      361 tgccatcatc ggcaagagcg ccgataatgt cctcggtccg accggacgcg atctgatcgg
      421 caagttgcgc cggatggcgc tcgacggcgg cccggtgcat gtcgcgcgcg agtctttcgt
      481 caggtcggac cgtggttttc atctcttcgc gcatcgctcc ggcggcctca tcattctgga
      541 actcgaaagc gcgccgccca gcgcccagcc tttgccaaat ctttattccg agctgcgcgc
      601 cgacatcgcg aggcttcagg agacgaagag cctggccgcg ttcttcgatc ttgcggtcga
      661 aaaaattcga aagtttaccg gcttcgaccg ggtgatggcc taccggtttg acgaggatgg
      721 cagcgggcac gtcgtcgccg agtcgaagcg cgacgatctt gaggcgtatc tcgggctgca
      781 ttaccccgcg accgacattc cgtcgcccgc gcggcggttg ttttcgctcg cctgggttcg
      841 tcatctcccg gatgtcgatt atgtgcctgt atcgctcgtt tcggcaaaga cctatacctc
      901 cggccctgtc gacatgagct tcgcgagcat cagaagcgtc tcggtgatgt actccgacta
      961 tctcaagaac atgggcgtga aggccaccat ggtcatgccg ctcatgaagg agggcaagct
     1021 ctgggggctc atttcggcca tgcatcacgc tgcgcctcgg cacgtccccc atgagacgcg
     1081 catggcggcc gagttcctgg cgcatacgct ctcgctcttg atgtcggcca aagaggatac
     1141 ggaggtgttc gaacgcgtcc tcgccatgag cgagacaacg gaccggctca tccaggcgct
     1201 atccgttgaa cccgatttcg gcaagacgct cgggacgccg gaaactctcg ccgatgtgct
     1261 gaaccaggtg gaggccgccg gggcggcagt ggtctctcag tccaaggtct cccttactgg
     1321 caagacgccc ccggaggagg cggtgcgcga tctggcgcaa tggatcggta atgagggcgg
     1381 gccgcttttc gcaacggatc gactgccttc gctctatccg ccggccgaag cattcgcgag
     1441 ggctgcaagc ggggtgctgg cggttcgcat ttcgcccgat cgccccgaat ttctcctgtg
     1501 gtttcgcccc gagcagatcg aagttgtcac ctgggccgga gatccgcata agccggtcga
     1561 tgtcagcgag gccgacggtt cggtgcgctt gcgcccgagg gggtctttcg cgctctggaa
     1621 agaaagcgtg cgcaatcgct ccacgccatg gcacgaaaat gaaaagaccg ccgccctcaa
     1681 attgcgcgtt ggcatcgccg atctcgtcct cggaggcgcg ggcaagatcg atcgtataag
     1741 ccgggagctt gaggcctccc acgccgatct ccacagcttc acccatgccg cgtcccatga
     1801 tctgaaggac catctgcacg gcattcacca tctcgcgtcc acgctgaagc gcaggcaagg
     1861 ggatgtgctg gatgaagagg ggcggcagca gattgccacc atcctcaagc tcacccaaag
     1921 gatggatggc ctgatcgacg cgcttctcga tcatgcgcgg gtggacaaaa ccgccatggc
     1981 gctcgaaagc gtcaatctgg acgaggtcgt cgattcagcg ctgctcggct taggccactc
     2041 tctggccgag ggcggcgttg atgtgcgccg gccctcccgg cttggcaccg cgctatgcaa
     2101 tcgcgagcgg atccgcgatg tcctcgt
//
LOCUS       DATMGX010000606         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_167918, whole genome shotgun sequence.
ACCESSION   DATMGX010000606 DATMGX010000000
VERSION     DATMGX010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167918"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(603..1376)
                     /locus_tag="VNR65_09890"
     CDS             complement(603..1376)
                     /locus_tag="VNR65_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19023.1"
                     /translation="MGKANGKARERSFTVDMWWTDLNDVEIGFFIHSRHRAKSREFTQ
                     DSDVIGEVLEGGERTALISYREGLWKSEKPGEKRLVLKLFTPSMNFRASMDMLVGRSV
                     QLSAGAGGFPVTAYSIHIDGLDQVIQLERSARKWPGMPEQFSFLLMENGEATLYTLRH
                     DWIDISGSYTLFDQTGATIARLTGHLFDLGGRWDVVLSEGCSPYLVTILQLVSAMLKF
                     NAAARRHVDRLTHGVTSGAIKLALEKHEADLYLNPRRSH"
     gene            1894..>2160
                     /locus_tag="VNR65_09895"
     CDS             1894..>2160
                     /locus_tag="VNR65_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ19024.1"
                     /translation="MRDDALQKMSPLKLEAVLYALSDPVRLHIVHSLAKRGELACFAA
                     VAGLDIAKSTQSHHYRILREAGVIAQRKEGVCFISTLRKSDLDTR"
BASE COUNT          485 a          620 c          617 g          438 t
ORIGIN      
        1 gggtcagtgc aacgcaacgg gtcagttcgt gcgcaatcaa aatctaactc aaaaaccgca
       61 agaaaaaacg cgaaaacgaa ccctcgcacg gctgcttacc aagtcggccg cgtccacgac
      121 cggcttcctg acttataaga atctacattt tccggcggct cttggaaaga gtggaacgcg
      181 ggccgggaaa aaggaggggg acggagcaac gccgatgggc gcctgggtct gcgcgaaggt
      241 ctattaccgc cccgacaaac tgaggcggcc acagatctcg ctgcataccg aacctctgcg
      301 cgtcaattac ggctggtgcg atgcgccagg cgaccggaat tataaccgcc aagtcacgct
      361 gccttatgcg gcgagcgctg aaaggctttg gcgcgacgat tatctgtacg atgtgatcgt
      421 cgtgctcaac tataacagca tgccgcgatc gcgaggccgg ggaagcgcaa tcttccttca
      481 tgtcgcccgc ccatcccttt cgccaacgga agggtgcatc gcgatgaagc gcgagcacct
      541 gctccggctg ctgcggatac tcacgccggg tgcggcgatc gccgcaggca aaacgcttgc
      601 gatcaatgcg agcgccgcgg gttcaagtag agatcggcct cgtgcttttc gagggcgagc
      661 tttatggcgc cgctcgtcac tccgtgggtc agccggtcca cgtggcggcg ggccgcagcg
      721 ttgaacttca gcatcgcgct gacgagctgc aggattgtga caagatatgg agagcaaccc
      781 tcgctcagga ccacatccca gcggcctccc agatcaaaga ggtgccccgt caaacgcgct
      841 atcgtggcgc cggtttgatc gaaaagcgtg taactgccgc tgatgtcgat ccaatcatga
      901 cgcaaagtgt agagcgtggc ctccccattc tccatcagca aaaacgaaaa ctgctccggc
      961 atacccggcc acttgcgggc ggagcgctcg agctggatga cttgatcgag cccatcgatg
     1021 tggatggaat aggctgtcac cggaaagccg cctgctcccg ccgaaagctg gacgctgcga
     1081 ccgacgagca tgtccatgct ggcgcgaaag ttcatggatg gcgtgaaaag cttgagcaca
     1141 agacgctttt cgcctggttt ttccgatttc caaaggcctt cgcggtaact gatcagcgcg
     1201 gtccgctcgc cgccctccag gacttccccg ataacatcgg aatcctgtgt aaattctcgg
     1261 gatttcgcgc ggtggcggga gtggatgaaa aatccgattt cgacatcgtt gagatcagtc
     1321 caccacatat cgacggtgaa tgagcgctcc cgcgcctttc cgttggcctt ccccatgtcg
     1381 ccccctatgc tcgaatcatt cgaagcaagc ttgcggtata ttggcgcaag tggcgcaaga
     1441 gaaagctcca caatccacaa ctggtttttg gagttggctc tgatacgcgc cccaatactt
     1501 cctcatgcca gccgtgagaa gattcgactc ctaccgtcca atggaacttc ccaagccagg
     1561 ggtcgtcttg tgatggtgca cattcggtta aaattggttt cgttttgtaa agccgcatat
     1621 aagtaattat ataagtagtt ttcctccacg acaacgggca cgggcaaagg cgcagtaact
     1681 tacgatccgt atgacacatc agaaatggag aacgtggctg cgcaaaatcg gggcggagac
     1741 gccgaaagac gaaaaagccg gagcgtgccc gtcctggagc aagcgtgcgc ttggagcgct
     1801 ggcattgcac ttgtagttta gtccctgccc ttctgcgcgg ctcgaaacaa aactaactgt
     1861 tgaactcacg gtaaggcgcg ccatcttttc gccatgagag atgatgcgct tcagaagatg
     1921 tcgcctctga agctcgaagc ggttctttac gcactgagcg atccggtgag gctgcatatc
     1981 gtgcatagct tggcgaaacg cggcgagctt gcatgcttcg ccgccgtggc gggccttgac
     2041 atcgcgaaat caacccaatc gcatcactac cggattttgc gtgaggccgg cgtcattgcg
     2101 cagcgcaaag agggcgtgtg cttcatttcc acgcttcgca agtcagacct cgacacgcgg
//
LOCUS       DATMGX010000607         2757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_417886, whole genome shotgun sequence.
ACCESSION   DATMGX010000607 DATMGX010000000
VERSION     DATMGX010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417886"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VNR65_09900"
     CDS             complement(<1..323)
                     /locus_tag="VNR65_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19025.1"
                     /translation="MVIADAAKEFDPATPPMEARQVDRLPSEPGWQFEPKWDGFRCLF
                     FRAGNRAELAGKSGKSLARFFPEIVSLVERLEPENFALDGELIVEANGALSFDALQMR
                     LHLAA"
     gene            470..1012
                     /locus_tag="VNR65_09905"
     CDS             470..1012
                     /locus_tag="VNR65_09905"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HWJ19026.1"
                     /translation="MKKSIIIMAACALITGPAAAQSIGEKTGINEVLGITPSTKDFVK
                     EVATSDMFEMDSSALAEANATDEATKSFAKQMVTDHEKTTKELTEAVGTNPSTPIPSE
                     LDSSHKSKLEKLKSLTGANFTKTYLDDQVSAHKDAVNLFERYAKGGDNATLKSWAEKT
                     LPTLKHHLEMAQSLDNKARS"
     gene            complement(1174..2541)
                     /locus_tag="VNR65_09910"
     CDS             complement(1174..2541)
                     /locus_tag="VNR65_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HWJ19027.1"
                     /translation="MTDDSPVTVSLPAVSRKKVTAAFDGGRLSSDSGVILLSLAERRR
                     AVARTLAARIDDPRDPAHITHTVEDVLTARMFAIACGYPDGNDFHFLRSDPAFKLACG
                     RLPDTGADLCSQPTISRWENAPTLKEIVRLTYALVDIWCASYSKPPVSVVLDIDDTLD
                     VVHGHQKLAEWNAHYDERCFLPIHIYDTATSRPVAVILRPGKTPSGKEVRGWLRRLIR
                     RIRRHWPNTRITIRGDSHYGREEAMSWCEANGVDYIFGLSGNVVLDRLVDAAADDIRV
                     RRAEGQYAVLRGYAETRYAAKSWAKKRRAVARIEASASQEDDMLRRGLDIRYVVTSLN
                     KGSAEYIYATLYCARGQAENLIKQHKAQTASDRTRCRSPLANQFRLILHTAAYWLLLD
                     LRDCAPSWNPARQSEFATIRLRLLKIAGRITETASRIRVSLASCCPEAELFSLVAFEL
                     QKSGP"
BASE COUNT          567 a          820 c          843 g          527 t
ORIGIN      
        1 ctcgcggcga ggtggaggcg catttgcagg gcgtcgaagg agagcgctcc gttcgcctcc
       61 acgatcaact ctccgtcgag cgcgaagttt tcaggttcaa gccgctcgac cagcgacacg
      121 atttccggga agaaccgcgc cagtgatttg cccgacttcc cggcaagctc agcccggttc
      181 ccggcccgga aaaagagaca tcggaagccg tcccatttcg gctcgaactg ccagccgggt
      241 tcggatggca ggcggtccac ttgccgcgct tccattggcg gcgttgcagg atcgaactcc
      301 tttgctgcgt cagcgatgac catcgcgagg ccttctcata atggcggtcc gcgtagcttt
      361 taggcgcttt gggccaccgg tttggcacga agatgtgaac agtagacgga actttggagg
      421 caggggcgca ttcgcccctt agtaatgttt cacttagatc gaaggtttca tgaaaaagag
      481 catcatcatc atggctgctt gcgcccttat cacgggtcca gcagctgcac agtccatcgg
      541 cgagaaaacc ggcatcaacg aggttctcgg catcacccct tcgacgaagg atttcgttaa
      601 ggaagtcgcg accagcgata tgtttgagat ggactcgagc gcgcttgcgg aggcgaatgc
      661 caccgacgaa gcgaccaaat ccttcgccaa acaaatggtt accgaccatg agaagacgac
      721 caaggagctg acggaagcag tcggaaccaa tccaagtacg ccgattccga gtgaactcga
      781 cagctcgcac aagagcaagc tcgagaaact caagagcctc acgggcgcga acttcacaaa
      841 gacgtacctt gacgaccaag tgagcgccca caaggatgcg gtcaacctct tcgagcgcta
      901 cgcaaagggc ggcgacaacg caaccttgaa atcctgggcc gaaaaaacgc tccccacact
      961 caagcaccat cttgaaatgg cgcaaagtct ggataataag gctcgtagct agcccacctt
     1021 attcgcccca cagcccgatt gcatgggggc ggtgactgga caatgccctt cggttccagc
     1081 aaaggtcata cctcgcgggc tgatttgagc gggtacgctg tgaacgcgtt gaaggttgtc
     1141 gatccgggct ccaggggctt cgcgccccgc cggtcacggc ccggatttct gcaattcgaa
     1201 cgcaacgagg ctgaacaact ctgcttccgg gcagcatgag gcgagcgaga ccctgatgcg
     1261 gcttgcggtt tcagtaatgc ggccggcgat tttcagcagc cgcaggcgga tggtggcgaa
     1321 ttcgctttgc cgggcggggt tccaggaggg cgcgcaatcg cggagatcga gcaacagcca
     1381 ataggcggcg gtgtgcagaa tgaggcggaa ctggttagca agcggcgaac ggcaacgggt
     1441 gcgatccgaa gcggtttgcg ccttatgctg cttgatcagg ttttcggctt ggccgcgtgc
     1501 gcaatagagc gtcgcgtaaa tgtattccgc gctgcctttg ttcagcgagg tgactacata
     1561 gcggatatcg aggccccggc gaagcatgtc gtcttcctgg ctcgcgcttg cctcgatgcg
     1621 cgccacggcg cggcgcttct tggcccagct tttcgcggcg tagcgcgtct cggcataccc
     1681 acgcagcacg gcatactgcc cttcggcccg gcgcacccgg atatcgtcgg cggcggcatc
     1741 cacaagcctg tcgagtacga cattgccgga aagtccgaag atgtaatcga cgccgttcgc
     1801 ctcacaccaa ctcatagcct cttcgcggcc atagtggcta tcgccgcgga tggtgatgcg
     1861 cgtgttgggc caatgccggc ggatgcggcg gatcaggcgg cgcagccagc cgcggacttc
     1921 cttgcccgat ggcgtcttgc cggggcgcag gatcaccgcc accggccggc tggttgcggt
     1981 gtcgtagatg tggatcggca gaaagcagcg ctcgtcgtaa tgagcgttcc attccgccag
     2041 tttctgatga ccgtgcacaa catcgagcgt gtcgtcgatg tccagaacca ccgagaccgg
     2101 cggcttcgag tagctggcgc accagatatc aaccagcgcg taggtgagcc ggacgatctc
     2161 tttcagcgtg ggcgcattct cccagcgcga gatcgtgggc tgggaacaca aatccgcgcc
     2221 cgtatctgga agtcgtccgc aggcgagctt gaaggctgga tcggacctga gaaagtggaa
     2281 atcattgccg tccgggtagc cgcaggcaat cgcaaacatg cgcgctgtga gcacatcctc
     2341 gactgtgtgg gtgatgtgcg ccgggtcgcg cggatcgtcg atccgagcgg caagcgttct
     2401 ggccaccgcc cggcggcgct cggcaagcga aagcaagatc acgccgctgt cagaggaaag
     2461 acggccgcca tcgaatgcgg ctgtgacttt cttgcgcgac acggctggca aggaaacggt
     2521 aactgggcta tcgtccgtca tggcgggtgt ggctgggctg tttgacctgc gctggatggt
     2581 cttgacaccc atttcctacg caaattcaac cccttgcgct acccccgcca gcccgattcc
     2641 tccgccccgt gaataagaac ggctagaagc gcttcccaca ttctcgctaa gggccatcca
     2701 ggcataaact tagggggctc cacggccccc ctttttttgt tgatacccat tggagtc
//
LOCUS       DATMGX010000608         4775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_205544, whole genome shotgun sequence.
ACCESSION   DATMGX010000608 DATMGX010000000
VERSION     DATMGX010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205544"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..698)
                     /locus_tag="VNR65_09915"
     CDS             complement(96..698)
                     /locus_tag="VNR65_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWJ19028.1"
                     /translation="MNHAQKRTLIAVAAVTLLTAACAKKVAVGPPPPPAPGPTPAVAS
                     KPAPPPPVHAATAKPPAATTPAPKYPNAVTRARIDTLLTKIEDAYFDYDKYSLRPDAM
                     KALQADSTELRDILKDYPDYKLTIEGHCDERGSAEYNVALGDRRAGAAKEYLVGIGIP
                     SAQLSLISYGKERPVCQEEDEACWQRNRRIHIVATAQAQK"
     gene            885..2210
                     /locus_tag="VNR65_09920"
     CDS             885..2210
                     /locus_tag="VNR65_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWJ19029.1"
                     /translation="MILSLGFGGLLICIVAAAVGTLVVLQRVRNTEAQSRKAFLERLS
                     ALDQIRSQIYLSGTYVRDFLLSPDPDTAKTQAAHLATLERETHTALDAYRRELEPEER
                     EPFLALRNEIDAYWRVLDATVQWSPQERNRLRFSFFYNELVPRRTAMLQVADRIASVN
                     ERGLTRSEERTTASADSLRRYLIWTFVGTLSGGLILALVTIGFTLRLERELDLRRADL
                     QELSTLLLRAQESERRALARELHDEIGQSLSAILMETEGAEYADPPAGVREHLDSIKG
                     LAEKTVGQVRDLALLLRPSMLDDLGLTPALNWHARETSKRTGLNVVVSADEAIDSLPD
                     EHRTCIYRLVQEAVNNAVRHANARTVEVAVRKEYQKVDVTVQDDGAGFDTRFLRGLGL
                     LGMEERVRRLGGRLTVGSEPGRGTLVHAVLPMAELDQRNGHDANSYLVG"
     gene            2182..2832
                     /locus_tag="VNR65_09925"
     CDS             2182..2832
                     /locus_tag="VNR65_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ19030.1"
                     /translation="MTPIRILLADDHTVVRDGLRAMLERETDMTVVAEAADGRESIRL
                     AESEAPDVVVMDLAMPNMNGMEATRRILAANSRVGVVILSMHQDESYVLGALKAGAKG
                     YLLKDSMRGEVVQAIRLVAQGRSFLTRKVARIMQEDYVNRLQQRGLEDRYDLLSDRER
                     EVLQIIAEGRANKEVANLLNISPTTVETHRAHILQKLDIHSVPELILYAVRKGIIS"
     gene            2933..4516
                     /locus_tag="VNR65_09930"
     CDS             2933..4516
                     /locus_tag="VNR65_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ19031.1"
                     /translation="MKNRIGEVLKTLRRFVLVAPSPALRYGLAGIAALAAVGARSALD
                     PVLGPYSQFPLGSLAVIVAARFGGRGPGLATTALSAFGILRFVIEPSDSLVVPNREAS
                     AGLALFVVVGTLLSLLVGQLRESLLSTARAEGDLRRASEQRELALAAAKLGAWDYRFE
                     RGEMFLDERCRNILGVTADLCFDDAVARIHGEERAAVIEAMERALAGVNGGAFEREFR
                     VVWPDNSVHWVASHGQVFFEGDGARRRAVRFVGVSTEITERRLADERLRQTQKLESIT
                     LLAGGIAHDFNNLLTVIMGSASSALAERPSCEHSQAILSASKRASYLTKQLLAYAGKG
                     AVIVRLLDLTELVSQSKGLLTLSVPKCVDISYNLSEDLPCLEADPSRIEQILMNLVVN
                     AGESIPSGSNGLIEIATSSCEVTAELARQRSEAYDVASGAYVCLQVRDNGIGMDQATL
                     SRVFDPFYTTKFTGRGLGLAAVHGIVRSRHGFIDVRSLPGAGTTFRVLLPASPKRRPT
                     EFAQPTTDQQRKWYANWRA"
BASE COUNT          896 a         1429 c         1520 g          930 t
ORIGIN      
        1 atatcctccg ttcgcctgcg tttcggatct tggggccgct cggaagttcg cgagggacca
       61 gtgccagagc gcttccaaac cacggcccca tccagctatt tctgtgcttg agccgtggcc
      121 acgatgtgga tgcggcgatt ccgctgccag caggcttcat cttcctcctg gcagaccggg
      181 cgttctttgc cgtaactgat caggctgagt tgagccgacg ggataccgat cccgaccagg
      241 tactctttgg cggcgccagc acgcctgtcg ccaagggcga cattgtactc ggcggagccg
      301 cgctcatcgc agtggccctc gatcgtcagt ttgtagtcgg gataatcttt caggatgtcg
      361 cgcagttcgg tcgaatcggc ctggagggct ttcatggcat cgggccgaag agagtacttg
      421 tcgtagtcga aatacgcatc ttcaattttg gtcagcagag tgtcaatgcg ggcgcgggtc
      481 accgcgttcg gatatttcgg cgccggcgta gtcgcagcgg gaggctttgc cgtcgcggcg
      541 tgcacgggtg gtgggggagc cggtttcgag gcaaccgcgg gcgtaggccc tggagccggc
      601 gggggcggtg gtccgaccgc gactttcttg gcacaagcgg cggtcagtaa cgtgacggca
      661 gcaacggcaa ttaaggttcg tttctgggcg tgattcatgt tggtccttcc tacttacgat
      721 tggacccgtt atcggccgcg agcggaatcc tgtctggaca ggattttttt gaaggcaagg
      781 cttgaactac actcagaagt ggaagaggtc gtgctcccat tttgcattcc tactcgggca
      841 gtccggtggc cggctcggcg gaaaagaacc gaaagccttc atggatgatc ctgtctctcg
      901 gctttggggg attgctgatc tgtatcgtgg cggccgctgt aggcacgctt gtggtgttgc
      961 agcgggttcg taacacagag gcccaatcgc ggaaggcgtt tctggaacgg ctcagcgctc
     1021 tcgaccagat tcgctcccag atctacttgt cgggcaccta cgtgcgcgac ttcctgcttt
     1081 caccggaccc cgatactgcc aaaacgcagg ctgcgcacct ggccacattg gagcgtgaaa
     1141 cccatacggc tctagatgcc taccgccgcg aacttgagcc cgaggaacgc gagccatttc
     1201 ttgcgctgcg caacgagatt gatgcctact ggcgggtact cgatgctacc gtccagtggt
     1261 cgccgcagga acggaaccgc ctgcgattct cgttctttta caacgaattg gtcccgcggc
     1321 ggacagccat gcttcaggtg gcggatcgca tcgcgtcggt caacgaacgg ggcctgacgc
     1381 gttccgagga gcgtaccacc gcctcggcgg acagtctgcg gcgttacctc atctggacct
     1441 tcgtgggcac cctcagcggc ggtctgatcc ttgcgctcgt gaccatcggc tttacgctcc
     1501 ggctcgaacg ggaactcgac ttgcgccggg ccgatctgca ggaactgtcc acgctgttgc
     1561 tgcgcgcgca ggaaagcgag cgtcgcgcgc tggcacgcga gttgcacgac gaaatcgggc
     1621 aatctctgtc ggcgatcctg atggagacgg aaggcgccga atatgcggat ccgccggccg
     1681 gcgtccgcga acacctcgat tcgatcaagg gcttggcgga gaagacggtg ggccaggtac
     1741 gagacttggc actgctgctt cgaccgtcga tgctggacga cctcggtcta acgccggcgc
     1801 tgaactggca tgcgcgcgaa accagcaagc gcactggact caacgtggtg gtctccgccg
     1861 acgaagccat cgacagcctg cccgacgaac accggacgtg catttaccgg ctggtgcagg
     1921 aggcggtgaa caatgcagtc cgccacgcca acgcgcgcac cgtagaagtc gcggttcgga
     1981 aggagtacca gaaggtggac gtgactgtac aggatgacgg agcgggtttc gatacccgtt
     2041 tcctgcgtgg cctgggcctc ttgggtatgg aagagcgggt acggcgactg gggggacgcc
     2101 tcacggttgg ttctgagccc gggcgaggga ccctggttca cgcggtactg ccgatggccg
     2161 aactggacca aaggaacgga catgacgcca attcgtatct tgttggctga cgaccacacg
     2221 gttgtccgcg acggcctccg ggccatgctg gagcgcgaga ccgacatgac cgtggtggcg
     2281 gaggcggccg acgggcgtga aagcatccga ttggccgaga gcgaagcgcc cgatgttgtg
     2341 gtgatggatc tggcgatgcc caacatgaac ggcatggagg ccactcgccg cattctcgcg
     2401 gcgaactcgc gcgtgggcgt cgtgatcctg agcatgcatc aggacgagag ctacgtgctt
     2461 ggggcgctca aggccggtgc gaaggggtac ctgctcaagg attcgatgcg cggcgaggtt
     2521 gtacaggcaa ttcggctggt ggctcaaggg cgctccttcc tgactcgcaa ggtcgcccgg
     2581 atcatgcagg aggactacgt caaccggctg caacagcgcg gactggaaga ccgctatgac
     2641 ctgctctcgg accgcgagcg cgaggtcctg cagattatcg ccgaaggacg cgccaacaag
     2701 gaagtcgcaa acctgctgaa tatcagcccg accacagtcg agacgcatcg cgcccatatc
     2761 ctccagaagc tcgatatcca cagcgttccg gaactcatcc tctatgccgt acggaagggc
     2821 atcatctctt gacgccttgc gcgaagccta gcccgtccgt cgatctggat cgttttcgcc
     2881 acgttaccga gcgatgcctg tctgaattcg gaatagggga gtggtagccg agatgaagaa
     2941 tcgaatcggc gaggtactca aaacgcttcg gcggttcgtc ctggtagccc cctcaccagc
     3001 gttaaggtat ggcttggccg gaattgcggc actggctgcc gtcggtgccc gttcagccct
     3061 ggatccggta cttggcccgt actcacaatt cccgcttggg tccctcgccg tgatcgttgc
     3121 agcgcggttc gggggtcgcg gacccggcct ggcgaccacg gcgctcagcg cgttcggcat
     3181 tctgcgcttc gttattgaac catccgattc cctggttgtc cctaatcgcg aggctagcgc
     3241 tggcctggcc ctgttcgtgg tggtcggaac cctcctcagc ctcctggtcg gacaactccg
     3301 cgaatcgctc ctctcaaccg cccgcgccga aggggatctg cgaagggcat ccgaacagcg
     3361 cgagctggct ttggctgcag cgaagctcgg agcctgggac taccgattcg aaagaggcga
     3421 gatgttcttg gacgaacgat gccgtaatat actcggcgtg accgcggacc tgtgcttcga
     3481 cgatgctgtt gcccggatcc acggcgaaga gcgcgcagcc gtcatcgaag caatggagcg
     3541 cgcgctggcg ggcgtcaacg gaggcgcgtt cgagcgggag tttcgcgtcg tatggccgga
     3601 caactcggtg cattgggtgg catcgcacgg gcaggtgttc ttcgaaggcg acggcgctcg
     3661 gcgccgcgcc gtccgcttcg tgggagtcag cacggagatc accgagcgca ggctcgccga
     3721 cgaacgtctg cgtcaaacgc agaaactgga gtccatcact cttctggccg gcggcatcgc
     3781 gcacgatttc aataatctcc tgaccgtgat catgggcagc gccagctccg cgctcgccga
     3841 acgcccgtct tgcgaacact ctcaggcaat actctctgcg tcgaaacggg cctcctacct
     3901 gacgaagcag ttgttggcgt atgcaggcaa gggcgcggtc attgtcaggc tattggatct
     3961 aacggaacta gtatcgcaat cgaaggggct tctcacgctt tccgtcccga agtgtgtgga
     4021 catcagctac aacctctcgg aggatctgcc ctgccttgag gcggacccaa gccggatcga
     4081 gcagatcctg atgaatctcg tcgtcaacgc gggtgaatcg ataccatccg gcagcaatgg
     4141 gctgatagag atagcgacca gcagttgcga agtcacggca gagttggccc gccagcgctc
     4201 cgaagcgtac gatgtcgcca gcggagccta cgtctgcctt caggtgcgag acaacggcat
     4261 aggcatggac caagctaccc tatcgcgggt cttcgatccc ttctacacga ccaaattcac
     4321 cggccgcgga ctcggcttgg ccgccgtcca tggaatcgtg cgcagccgcc atggctttat
     4381 tgacgtccgg agtttgcccg gcgctggcac tacatttcgc gtgctcctgc cagcctcccc
     4441 aaagagacgg ccaacagagt tcgcccagcc caccacggac cagcagagga agtggtacgc
     4501 aaactggcgt gcatgactct gagagcgcgg ataggaggtt ctgccgccaa tgacggctat
     4561 ttcctggcgt gtcctcgttc gggcccacgg catcagcaga tccattacgg gatcgttcgc
     4621 acgggagcgt gatgccggcg gatactatcg acctaatgca gacgcccaga ctgcattacc
     4681 tgggccactg cgatcagcag gagggccacc aaaagtccaa ccgccgcagt ccaaatgtag
     4741 agggggtcca cgttccgcca gacgccaagg tgtct
//
LOCUS       DATMGX010000609         2312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_360221, whole genome shotgun sequence.
ACCESSION   DATMGX010000609 DATMGX010000000
VERSION     DATMGX010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360221"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VNR65_09935"
     CDS             <1..681
                     /locus_tag="VNR65_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWJ19032.1"
                     /translation="RELTIKGQEILTADKVAIRVSIIVQFRVSDPKAAIQEVEKYEER
                     LYSDVQLAARRSLASMTLEEILTNRNRLSEDIRTDVKESAARYGVLVLRADVKDLIFP
                     GNLQEIMNKVLAAERTSQAQLIEARTKAQTQEIEAEAQAGIRKVRAAAEAEAARLQAQ
                     ASAEAQRIKTGAEVKALRESQEAAQAYATHPALLRLRELEALKDIAQSANARIYIDFK
                     KHEGGEGE"
     gene            complement(717..1085)
                     /locus_tag="VNR65_09940"
     CDS             complement(717..1085)
                     /locus_tag="VNR65_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ19033.1"
                     /translation="MTPPASILVVEDEFLVAMQIEAVLRGVGWSVIGPAGTLAKAVSI
                     AQTATCDGAVLDVNLRGERVDDVAAILSERGIPFVFASGYGRENLPAAFRDSTAFLAK
                     PFSDQTLVQAVRGLLQHKAS"
     gene            complement(1082..>2312)
                     /locus_tag="VNR65_09945"
     CDS             complement(1082..>2312)
                     /locus_tag="VNR65_09945"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWJ19034.1"
                     /translation="ADLKTDLAAMHQLLAGSIPNYTMEKRYIRKDGGIIWGNLTVSLV
                     RKADGSTDYFVSVIDDITVRKQAATELAESRSRLSVVVDSAMDAIISIDSRQNVVLFN
                     EAAERMFQRKAADVIGHSLEQLLPTRFASRHSRHIDAFAQTGVSNRAMDNLGALSALR
                     ADGAEFPIEASISQTSIAGEKLFTAIIRDITERKRAENIQRLLLAELDHRVKNTLATV
                     QAIAMQTLNAKMEPAAFVESFSGRIQALGRAHGLLSQSGWQGADLAMLVNEQLMLGVP
                     GVDSRIFTNGPHVMLEPQAALHLGLVLHELGSNARKYGCLSQPQGRLAAEWWLNGDSG
                     GPMLRLRWLESDGPHVDAPKRRGFGTVLIERSLHYALGGEAELDFAPGGLTCNIYLPL
                     AKAGRGAYSVKPSESAS"
BASE COUNT          473 a          709 c          705 g          425 t
ORIGIN      
        1 cgcgagctta ccatcaaagg tcaggagatc ctgacggccg acaaggtcgc catccgggtc
       61 agtatcatcg tgcagtttcg cgtctccgac ccgaaggctg cgatccagga ggttgagaaa
      121 tacgaggagc gcctctacag cgacgttcag cttgcggccc gccgctcact cgcatcgatg
      181 acgctcgaag agattctgac gaaccgcaat cgcttgagcg aggacattcg taccgacgtg
      241 aaggagagcg ccgcccgcta cggcgtcttg gtccttcgcg cggacgtcaa ggacctcatt
      301 tttcccggca acctgcaaga gatcatgaac aaagtgctgg ccgcggagcg gacgagccag
      361 gcgcagctta tcgaggctcg caccaaagcg cagacgcaag agatcgaggc cgaagcgcag
      421 gcggggatcc gaaaggtcag ggctgccgcc gaggcggaag cggcgcggct tcaggctcaa
      481 gcaagcgccg aggcacagcg gatcaagacg ggggccgaag tcaaagcgct ccgcgagagc
      541 caggaggcgg cccaggccta cgccacgcac ccggcgcttc tgcgattgcg tgagctggaa
      601 gcgctaaaag acatcgcaca atccgccaat gcccggattt atatcgattt caagaagcat
      661 gagggaggcg aaggcgaata gcactggccg gattcgtcaa cggcgctggt tagctgttat
      721 gaagccttat gctgaagaag gccgcgcacg gcctgcacga gcgtttgatc gctaaacggc
      781 tttgcaagga atgccgtgct gtcccgaaag gcggccggca gattttcgcg cccatagccc
      841 gatgcaaaca cgaaggggat gccgcgctct gaaagaatag cggcaacgtc gtcgacgcgc
      901 tcgccccgga ggttgacatc gagcaccgca ccatcgcacg tggcggtttg cgcgatactc
      961 acggccttgg cgagcgtacc cgctggcccg atcacgctcc accctacccc tcgcagaaca
     1021 gcctcgatct gcatggcgac gagaaactcg tcctcaacca cgagaatgct tgcaggtggc
     1081 gtcatgatgc gctctccgaa ggtttgacgc tataagcgcc gcgccctgct ttcgccaggg
     1141 gaaggtaaat gttgcaggtg agcccaccag gggcgaagtc aagctccgcc tctcccccca
     1201 gtgcatagtg caagctgcgt tcgatgagga cggtgccaaa ccctcgtcgt ttgggtgcgt
     1261 ccacatgcgg tccgtcgctc tccagccacc gcagcctcag cattggacct cccgagtcgc
     1321 cattcaacca ccattccgcc gccaagcgcc cctgcggttg cgacaggcag ccatattttc
     1381 gcgcgttgct tccgagttcg tgcaaaacca agcccaaatg cagcgccgcc tgcggctcga
     1441 gcatgacgtg cggtccgttg gtgaaaatcc ggctatccac acccggcacg ccaagcatga
     1501 gttgctcatt cacgagcatg gcgagatcgg cgccttgcca gcccgactgg cttaacaagc
     1561 cgtgggcgcg tcccaaggcc tggatacggc cgctgaaact ctcgacgaat gcggcgggct
     1621 ccatcttcgc attcagggtc tgcatggcga tcgcctgcac ggtggccagc gtgttcttga
     1681 cgcggtggtc gagctcggcc agcaacagac gctgaatgtt ctcggcccgt tttcgctcgg
     1741 tgatgtcgcg tatgatggcg gtaaagagct tttctccagc gatggacgtt tgcgagattg
     1801 acgcttcgat cggaaactcc gcgccgtccg cccgaagcgc gctcaaagcg ccgagattgt
     1861 ccatggcgcg gttgctgacc ccggtttggg caaaggcatc gatatgccgc gaatgacggc
     1921 ttgcaaaccg cgttggcagg agctgctcga gggaatgccc gatgacgtcc gcggccttgc
     1981 gctgaaacat gcgctccgcg gcctcgttga aaagaacgac attctggcgg gagtcgatgc
     2041 tgattatcgc atccattgcg gaatccacga ccacgctgag gcggcttcgg ctctccgcaa
     2101 gctcggtggc agcctgtttg cgcaccgtga tgtcgtcgat gacgctaaca aagtaatccg
     2161 tgcttccatc agccttgcgg acgagtgaga ctgttagatt gccccagatg atccccccat
     2221 ctttgcggat gtagcgcttc tccattgtgt aattggggat gctgccggcc agcaactgat
     2281 gcatggccgc aagatcggtc ttgagatcgg cc
//
LOCUS       DATMGX010000610         2669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_32996, whole genome shotgun sequence.
ACCESSION   DATMGX010000610 DATMGX010000000
VERSION     DATMGX010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32996"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VNR65_09950"
     CDS             <1..1122
                     /locus_tag="VNR65_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19035.1"
                     /translation="TELLAKDSAEKSQTAIREIERLRQDAQSHTQKAVAELQANFTSL
                     SDQVSTQLSTLSSKFSDTTRVVRDTTRRAAVDLESAQNELQRHAKVLPETAKQSATAM
                     RRALQDQLSALDALSDLANRHAYSSAVSAPEQRGEPQRREQLPPPPPPPTPEAALPPP
                     PRPAFDYGQPPSEKPSWSEPQPQQLPRPAPAAPVEVAQIPAPQPSFPEGQSPERRNQW
                     SLGDLLARASEDDTAFFDRDESFGLPPQTQPYAPRGGNMPDDLGMDFTMSDIASCIDE
                     RRVMEIWQRLKRGEMDVLTQRGLYPRQGQQIVDRVQRRYETDTTFRGIVDRYLSDFEK
                     MLQDLSRNDPRGGTVQSRLASDEGRIYFVLAHISGRLGA"
     gene            complement(1214..2002)
                     /gene="xth"
                     /locus_tag="VNR65_09955"
     CDS             complement(1214..2002)
                     /gene="xth"
                     /locus_tag="VNR65_09955"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWJ19036.1"
                     /translation="MLTVATWNVNSIKQRETACAQWLRRASPSILCLQELKCMTEGFP
                     RAAFEELGYNCAVSGQKSFNGVAILSKFPIDETIVGLPGDPSDEQSRYIEAVISIPGG
                     KAIRVASIYAPNGNPAPSPKLDYKLAWLARLKAHTETLLAFEEPFVLAGDYNIIPRPG
                     DVYNPANWVEDALFRLESRQAFRRIMNLGLTDAVLACDSSAGQYTFWDYQAGAWQKNL
                     GLRIDHILLSPHALAHLRCTEIDRRPRAWDKPSDHTPVSATLDL"
     gene            complement(2012..2347)
                     /gene="erpA"
                     /locus_tag="VNR65_09960"
     CDS             complement(2012..2347)
                     /gene="erpA"
                     /locus_tag="VNR65_09960"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HWJ19037.1"
                     /translation="MTTDANISVSDRAANRIREIMAGDGGQNALRISVQGGGCSGFRY
                     DFEIVSQTNADDLIIEKSGARVAIDPVSVPFMAGSEIDFVDDLIGASFKIVNPNATAS
                     CGCGTSFSV"
     gene            2429..>2669
                     /locus_tag="VNR65_09965"
     CDS             2429..>2669
                     /locus_tag="VNR65_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837597.1"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HWJ19038.1"
                     /translation="MKRMPELASYAAHPEESRGRLYDEAPCPTRNVYQRDRDRIVHST
                     AFRRLIHKTQVFIYHEGDHYRTRLTHSLEVAQIGRT"
BASE COUNT          557 a          850 c          765 g          497 t
ORIGIN      
        1 acggaactcc tcgccaagga ttcggcggaa aaatcgcaga ctgcgatccg cgagatcgaa
       61 cggttgcgcc aggatgctca gtcgcatacg cagaaagcgg ttgcggaact tcaggccaac
      121 ttcacctcgc tttccgatca ggtctccacc caactctcga cgttgtcttc gaaattctcc
      181 gacaccacgc gcgtggtccg tgatacgacg cgccgtgctg ccgtagacct cgagtcggct
      241 cagaacgagt tgcagcggca cgccaaggtt ctgccggaaa cggcaaagca gagcgccacc
      301 gctatgcgcc gcgcactgca ggatcaactt tcggcactcg atgccctgtc cgatttggcc
      361 aatcgccacg cgtactccag cgcggtttct gcgccggaac agcggggcga gcctcagcgg
      421 cgcgagcagc ttcctccccc tcctccgccg ccgaccccgg aagcagcgct cccgccgccg
      481 ccccgacctg ccttcgacta cggccagccc ccttcggaga agccctcgtg gtccgagcca
      541 cagccccagc agcttccccg cccggcaccc gcagcgcccg tagaggtagc gcaaatccct
      601 gctcctcagc cgtccttccc tgaaggccag tctcctgagc gccgtaatca gtggtccctg
      661 ggcgatctat tggcccgtgc ttccgaggat gacaccgcct tcttcgacag agacgagtcc
      721 ttcgggcttc cgccccagac ccagccctat gcgccgagag gcggaaatat gcccgacgat
      781 ctgggcatgg atttcaccat gagcgatatc gcatcctgca tcgatgagcg gcgcgtcatg
      841 gaaatctggc aaagattgaa gcgcggagag atggatgtgc tcacccagcg tgggctatac
      901 ccccgccagg gccagcagat cgtggatcgc gtgcagcggc ggtatgaaac ggatacgacc
      961 ttccgcggga tcgtcgaccg ttatctgagc gacttcgaga agatgttgca ggatctgagc
     1021 cgcaacgacc caaggggagg aactgtccag tcgcggctcg cttcggatga agggcgaatc
     1081 tacttcgtgc tcgcccatat cagtggaagg ctcggcgctt agatcaaccc gcctctaatt
     1141 ggaaccgaaa gtgctagaga agcccgcgtc caaatggagc gggcttctat aatttggggg
     1201 cgctgaagcg cgctcacaaa tcgagtgtcg cggaaactgg cgtgtggtcg gaaggcttat
     1261 cccaggcgcg agggcgccgg tcgatttccg tacatctgag atgcgcaagg gcatgaggcg
     1321 ataacaggat gtgatcgatc ctgagaccca ggttcttctg ccaggcgccc gcctgataat
     1381 cccagaatgt atactgtccg gcgctcgagt cgcaggcgag cacggcatcg gtaaggccaa
     1441 ggttcatgat ccgccggaag gcctgtcgag actccagcct gaacagagcg tcctcaaccc
     1501 agttggccgg attatagacg tcgcccggac gcggaatgat attgtagtct ccggccagca
     1561 cgaacggctc ttcaaaggcg agcagcgttt ccgtgtgcgc cttcaaccgg gccagccatg
     1621 caagcttata atcgagcttc ggagagggcg ccggattgcc gttcggcgca taaatggaag
     1681 ccacccggat cgcctttcca cccggtatcg agatcacagc ctcgatgtag cgggactgtt
     1741 catcgctcgg gtcaccgggc aatcccacaa tcgtctcgtc tatgggaaac ttggaaagga
     1801 tggccacgcc attaaaagac ttctgacccg acaccgcgca attatagcca agctcctcga
     1861 aagccgcgcg cggaaacccc tccgtcatgc acttcagttc ctgcaagcaa agtatggacg
     1921 gagaggcacg tcgcagccat tgcgcgcaag ctgtctccct ctgcttgatc gagtttacat
     1981 tccatgtggc gacagtgagc acggcagcgc cctagacgga aaaggatgta ccgcagccgc
     2041 aggacgctgt cgcgtttgga ttgacgatct tgaaggaggc gccgatcagg tcgtccacaa
     2101 aatcgatttc cgaacccgcc atgaacggaa ccgataccgg atcaatcgcc acccgcgcgc
     2161 cggacttttc gatgatgaga tcatccgcat ttgtttggga tacgatctcg aaatcgtagc
     2221 ggaaccccga acagccgccg ccttggacgc tgatgcgcaa cgcattttgg ccgccgtcgc
     2281 ccgccatgat ctcccgaata cggttggcgg cgcggtcgct cacgcttatg ttggcatccg
     2341 tggtcattgg aattgttcgc ctctcttgaa cattgcctag aagtttggac gttcgcgcct
     2401 cgttgtcaac cggcgtgagc cgaaggggat gaaacgcatg ccggaattag catcctacgc
     2461 tgcgcatccg gaagagagcc gcggacgcct ttacgacgag gccccctgcc ccacccgcaa
     2521 tgtctatcag cgcgaccgcg accgcattgt ccactcgacg gcgttccggc ggctcatcca
     2581 caagacccag gtcttcattt atcacgaggg cgatcactac cgcacaaggc tgacccacag
     2641 cctggaagtg gctcagatcg gccgcacgc
//
LOCUS       DATMGX010000611         4688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_352845, whole genome shotgun sequence.
ACCESSION   DATMGX010000611 DATMGX010000000
VERSION     DATMGX010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352845"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /locus_tag="VNR65_09970"
     CDS             <1..1959
                     /locus_tag="VNR65_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HWJ19039.1"
                     /translation="NAQRLWGQPNASPYLKDAFHAHLIAGRGEAVNPAKTGTKAAAHY
                     VLDVPAGGSKTIRLRLAAAKTKDPFGGLEKTFQSRIADADEFYQRIAPNNFTEDQRRV
                     HRQALAGMLWSKQFYYFDLEKWLREHKSHPLLESARHDVRNTEWFHMLNADVISMPDK
                     WEYPWYAAWDLAFHTVSLALVDFDFAKEQLLLMLRSLYFHPSGQIPAYEWNFSDVNPP
                     VHAFATLWLFKYEKQLGRADLRFLERSFQGLMLNFNWWVNRKDPAGRNVFAGGFLGLD
                     NIGLFDRSAPLPTGGSLEQADGTAWMAFYCHNMLEIALILAEHDEQYEDIAFKFLQHF
                     MWIAYAMDRLGEHHDEMWDEQDGFFYDVLRLPDGQAMRLKVRSLVGLLPLCASTVFDA
                     DALTRYPKLMELIALFRKRHPELVSQVAPTDEGYIGYKGRRLLSILNKKKLERVLGYM
                     LDENEFLGPYGIRSVSRRHLDHPFVFHVGAEEYKVQYLPAESNTGMFGGNSNWRGPVW
                     MPVNTLIVRGLVNLYSFYGDEFKVQCPTGSGRYMTLFEVAQELVRRLSGTFLRDANGR
                     RQVYGGMAKFQDDPHWRDLILFHEYFHGDDGAGLGASHQTGWTGLIAPLLDLFARVDA
                     KTLLESERERLMARVVKEQVGGEPIGGK"
     gene            1969..3450
                     /locus_tag="VNR65_09975"
     CDS             1969..3450
                     /locus_tag="VNR65_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWJ19040.1"
                     /translation="MSDLRYPSLYQINTRPWLTELSHALGKRATLDDIPDAELARLAE
                     TGFDWVWFLSVWQTGPAARAISRANADWRREFAATLPDLKDEDIGGSGFAIQSYTVHR
                     DLGGDAALARLRQRLQKRGLKLMLDFVPNHMAPDHPWIDEHPEYFVAGSEADLARSPR
                     NYRRVQTKDSPLVLAYGRDPYFDGWPDTLQLNYGNPDLQWAMIGELQRIAGQCDAVRC
                     DMAMLVLPEVFERTWGMRADLFWPKATESVRSQHPNFKFMAEVYWDLEWTLQQQGFDY
                     TYDKRLYDRLREGHARPVREHLHAGLDYQNKLARFLENHDEPRAAATFPREVHEAAAV
                     ITFLSPGLRFFHQGQFQGRKKRISPHLVRAPEEAIDQPLEQFYERLMSVLRRPVVRDG
                     QWQLLDCTPAWDGNWTWDCYLAFAWQGSDGERLLVTVNYAPNQSQCYVRLPFADLGNG
                     QWRLQDLLGDATYDRDGNDLQARGLYLDEPPWRCHAFTMSNAK"
     gene            3645..>4688
                     /locus_tag="VNR65_09980"
     CDS             3645..>4688
                     /locus_tag="VNR65_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19041.1"
                     /translation="MIDNSKPPTDGDLKGSEGIVYREASVMTANKGCMRLWSQFRRGL
                     LVLLAVAVAGCAAPVGVTKVSVEQATRARTQSVLTNGRPSPASLQFLTRLDLVERARE
                     DPEGTLTILHAGLGKADDSARLLALAELSFMYGQRSGDRTYDLAAAAYAWAFLFPKEL
                     GKRPGRYDIRVPLAIGIYDWAITEGLSEGEGEAEVLDLSARSVDLPFGTLELSRAAAG
                     FYYGKSQLTDFVALADLEVRGLRNRYRQPGIGAALAAKASPTGDDRVDRWLSPRGWVP
                     VTVVLRFDDLRQGMAGGTLRATIEVHDALSDPVIAIDGHDVPLESDYTAAIAWGLEGS
                     PFWDFEFAGFRRGD"
BASE COUNT          862 a         1409 c         1501 g          916 t
ORIGIN      
        1 aatgcgcaac gtctctgggg gcagcctaac gcatcgccct acctcaagga tgcattccac
       61 gcccacctca tcgcgggacg cggcgaggcg gtcaatcccg ccaagaccgg gaccaaggcc
      121 gcggcccact atgtcctcga cgtgcccgcc ggcggcagca agaccatccg gttgcgtctg
      181 gcggcggcca agacaaaaga ccccttcggc ggtttggaga aaaccttcca gagccgcatc
      241 gccgacgccg acgaattcta ccagcggatt gcgcccaata acttcaccga agatcaacgc
      301 cgggtgcatc gccaggcgct ggcgggaatg ctgtggagca agcagttcta ctacttcgac
      361 ctggagaaat ggctgcgcga gcataagagt catccgctgc tcgagtccgc tcgacacgac
      421 gtgcgcaaca cggagtggtt ccacatgctg aacgcggacg tgatctccat gccggacaag
      481 tgggagtatc cgtggtacgc ggcctgggat ctggcgttcc acacggtttc tctggcgctg
      541 gtggatttcg atttcgccaa ggagcagctg ctgctgatgc tgcgcagcct gtatttccac
      601 ccgagcggtc agattccggc ctacgagtgg aatttcagcg acgtgaatcc gccggtgcac
      661 gcctttgcca cgctgtggct ctttaaatat gagaagcagc tgggccgggc tgacctgcgg
      721 ttcctggagc gctcgtttca ggggttgatg ctcaatttca actggtgggt caatcgcaag
      781 gacccggcgg ggcgcaatgt cttcgccggc ggctttctgg ggctcgacaa cattggcctt
      841 ttcgaccgca gtgccccgct tccgacgggc ggatcgctgg agcaggcgga cgggacggcg
      901 tggatggcgt tttactgcca caacatgctg gagatcgccc tgattctggc cgaacacgac
      961 gagcagtacg aggatatcgc cttcaagttc ctccagcact tcatgtggat tgcctacgcg
     1021 atggaccgcc tcggcgagca tcacgacgag atgtgggatg agcaggatgg ctttttctac
     1081 gatgtgctgc ggctgccgga cggtcaggcg atgcgtttga aagtgcgctc gctggtggga
     1141 ctgctgccgc tgtgcgcatc cacggtgttc gatgcggacg ccctgacgcg atatccgaaa
     1201 ctgatggagc ttatcgccct gttccgcaaa cgccaccccg aattggtctc tcaggtggcc
     1261 ccgaccgatg agggttacat cggatacaag ggacggcggc tgttgtcgat cctcaacaag
     1321 aagaagctgg agcgggtgct cggttacatg ctggatgaga acgagtttct cgggccgtac
     1381 ggcattcgtt cggtctcgcg ccgtcacctg gatcatccgt ttgtgtttca tgtcggtgcc
     1441 gaggagtaca aggtgcaata cctgccggct gaatcgaata ccggcatgtt cggcggcaac
     1501 tccaactggc gggggccggt gtggatgccg gtcaatacgc tgatcgtccg ggggctcgtg
     1561 aacctgtact ctttctacgg cgacgaattc aaggttcaat gcccgaccgg ttccggccgc
     1621 tacatgacgt tgttcgaagt ggcgcaggag cttgtccgcc ggttgagcgg aaccttcctg
     1681 cgcgatgcca atggacgacg gcaggtctac ggcgggatgg ccaagttcca ggacgatccg
     1741 cattggcgcg atctgatcct gtttcatgag tacttccacg gtgacgacgg cgctgggctc
     1801 ggggccagcc accagacggg gtggaccggt ctcatcgccc ctctcctgga cttgtttgcg
     1861 cgtgtcgacg ccaaaaccct gctggagagc gaacgggagc gcctgatggc ccgggtcgtc
     1921 aaggagcagg tgggtggaga gccgattggc gggaaatagg cagggaacat gtccgatctt
     1981 cgctatcctt cactgtacca aatcaacacc cgcccctggt tgaccgagct gtctcacgct
     2041 ctgggcaagc gcgcgacgct ggatgacatt ccggacgccg agcttgcgcg cctggccgag
     2101 acgggttttg actgggtctg gttcctgagc gtctggcaga ccggccctgc ggcgcgggcg
     2161 atctcgcgcg cgaacgccga ttggcgccgc gagttcgcgg cgacactgcc ggatcttaag
     2221 gacgaagaca tcggcggctc cggattcgcc atccaaagct acaccgtgca tcgcgatctg
     2281 gggggcgatg cggcgctcgc acgtttgcgg cagcggctgc aaaagcgcgg gctgaagctg
     2341 atgctcgact tcgttccgaa ccacatggct ccggatcacc cgtggatcga tgagcacccg
     2401 gaatactttg ttgccggcag cgaggccgat ttggctcgct cgccgcgcaa ctaccggcga
     2461 gttcaaacaa aggacagccc gttggtgctc gcctacggcc gcgatccgta cttcgatggc
     2521 tggcccgaca cgctgcagct caactacggc aacccggact tgcagtgggc gatgatcggc
     2581 gagcttcaga gaatcgccgg gcaatgcgac gcagtccgct gcgacatggc catgctggtc
     2641 ctgcccgagg tgttcgaacg cacgtggggc atgcgggccg atctgttctg gcccaaggcg
     2701 accgagtccg tgcggagcca acatccgaac ttcaagttca tggccgaggt ttactgggac
     2761 ctggagtgga cgctccagca gcagggtttc gactacacct acgacaagcg cctctacgac
     2821 cgcctgcgcg agggtcacgc ccggccggtg cgcgagcacc tgcatgcggg attggattac
     2881 cagaacaagc tggcccgctt cctggaaaac cacgacgagc cgcgcgcggc ggcaacgttc
     2941 ccgcgggagg tgcatgaagc ggcggcggtc attacgttct tgtccccggg tctgcgcttc
     3001 tttcaccagg ggcaattcca ggggcgcaag aaacgcatct ccccgcattt ggttcgagct
     3061 ccggaggaag cgatcgacca gccgctggaa cagttttacg agcggctgat gtccgtgctg
     3121 cgtcggccgg tggtgcgcga cggccagtgg cagctgctcg actgtacccc tgcctgggac
     3181 ggcaactgga cctgggattg ctatctggct tttgcctggc aaggctctga cggcgagcgg
     3241 ctgctggtga cggtgaacta cgcgcccaac cagagccagt gttacgtccg gctgcccttt
     3301 gcagacctcg gcaacggcca atggcggctg caagacttgc tcggtgatgc cacctacgat
     3361 cgggacggca acgacttgca agcccgcgga ttgtatctcg acgagccgcc ctggcgttgc
     3421 catgccttta cgatgtccaa tgcaaaatag ggccaggcac ggcatgatgc agatgcggca
     3481 atgccgccca cgagcaggtc cgttcgagca tgcgtcaaat tcgatattta cccattttat
     3541 atgcttggca gatcgttggc aggtcgcacc tcggcaatct atcgggtttg acgattgttc
     3601 tgcgagttca tcatcgacac aggaaagcgc taagggatac ggacgtgata gacaatagta
     3661 agccgcctac tgatggcgac ctcaaaggct cggaggggat cgtctaccgc gaggcaagcg
     3721 tgatgactgc aaataagggg tgcatgaggc tatggtcgca attccggcgc ggactccttg
     3781 tgctgcttgc ggtggcagtc gccggctgtg cggcgccggt cggtgtcacc aaggtaagtg
     3841 tcgaacaggc tacccgcgcc cgtacccaga gtgtgctgac caacggcagg ccgagcccgg
     3901 cttcgctgca gtttctcacc cggctcgatc ttgtcgagcg tgcccgggag gatcccgagg
     3961 gcacgctcac gatcctgcac gccgggcttg gaaaagcaga cgattccgcc cgcctgctgg
     4021 ccctcgcgga gctgtctttc atgtacggcc aacgaagcgg agatcgcacc tatgatctcg
     4081 cggcagcggc ctatgcctgg gcgttcctat tccccaaaga actcggaaaa cgcccgggcc
     4141 gctacgatat tcgggttccg ctcgcgattg gcatctatga ctgggcaatc actgaaggct
     4201 tgagcgaagg ggagggcgag gcagaagtcc tcgatctgtc cgctcgctca gttgacctgc
     4261 cgttcggaac cctggaactt tcgcgcgctg cggccggctt ctactacggt aaaagtcaac
     4321 tgacggattt tgtcgcgctc gccgatcttg aggtgcgcgg gctgcgcaac cgctaccggc
     4381 aaccgggcat cggcgcagcg ctggccgcga aggcatcgcc caccggcgat gatcgggtgg
     4441 accgttggct ttccccgcga ggctgggttc cggtcacagt cgtcctgcgc ttcgatgatc
     4501 tccggcaggg catggcaggc ggaactttac gagccacgat cgaggtgcac gacgcgctgt
     4561 ccgatcccgt cattgcaatc gacggtcacg atgtgccgct cgaatccgat tacaccgcgg
     4621 cgatagcctg ggggctcgag ggctcgcctt tctgggattt cgaattcgct ggcttccgcc
     4681 gcggcgac
//
LOCUS       DATMGX010000612         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_79948, whole genome shotgun sequence.
ACCESSION   DATMGX010000612 DATMGX010000000
VERSION     DATMGX010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79948"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1626
                     /locus_tag="VNR65_09985"
     CDS             115..1626
                     /locus_tag="VNR65_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWJ19042.1"
                     /translation="MKAIQLLQARNTISRYDQRVHQELVFVMLVENIGFDKLVEVHWA
                     GEDKVWHTLRAEYHSSGGTNREVWRAQATFSPSDEASLPGDVEFALRYHVLGEDYWDN
                     NESRNYFSNADSGVLLEQSARLLNIDFNPILQAGQRYCPITVAVRHSLQPKRVEIHWT
                     TDNWRSNQVTPCFFYRMHWDKWRRSRARNPNRYDTSIWISQINADDAFRVQYAIGCKT
                     PSQTFWDNNFGHNYVARRRRLKILTLNLHCYQEENQDAKFSQIARAIDDLDVDVVCLQ
                     EVGEQWGNGAGDWESNAAKIIRDRLRQHYHLQTDWSHIGFDRYREGIAVLSRYDFLMT
                     DAGYVSSSQDVHSIDSRKVVMVQLHVPYMGVVNVFSAHLSWPSGGFFEQFDRLRAWAN
                     HKHGNHLAATFLCGDFNIKTGSEGYQAVVRTREYEDQYLAASSPNAFEKIFRQQSSDI
                     DRRLAKDGRIDFVFMQKHSSLQAVAARELFTNDDDYGRVSDHTGYCVEFEPNW"
     gene            2009..>2317
                     /locus_tag="VNR65_09990"
     CDS             2009..>2317
                     /locus_tag="VNR65_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19043.1"
                     /translation="MPFAERYAFPIDAEIGTSIPRTNDFKDLFYVRWGSIRFDVLWGD
                     ELNLKVVLNIYRPDNICERFIVDTDAYHVQWNRHKRVTRDFYLHPFPLKLGPVRRVTF
                     S"
BASE COUNT          508 a          698 c          660 g          451 t
ORIGIN      
        1 ctccttcggg tcacgggctg ggattttgtg gcgcctgcga gctaggccgc tcaccctcta
       61 aaggcagacc gacccgagat tcggcgaaaa agaagtgttg gggcccctga gaccatgaaa
      121 gccatccaac tcctccaggc aagaaacacc atctcgcgat acgatcagcg ggtccatcag
      181 gaactggtgt ttgtcatgct cgtcgagaac atcgggttcg acaagctggt ggaggttcac
      241 tgggccggcg aggacaaggt ttggcatacg ctccgggccg agtatcacag ctccggtggc
      301 acgaaccgag aggtctggcg cgcccaggca acgttcagtc cgtcggacga agcttcgctg
      361 cctggcgatg tcgagtttgc gctgcgctac cacgttctgg gagaggacta ctgggacaac
      421 aacgaatcgc gcaactactt ttcgaacgcc gactccggcg tgctgctgga gcagagcgcc
      481 cggttgctca acatcgattt caatccgatc ttacaagccg ggcaacgcta ctgtccgatt
      541 acggtcgccg tacgccactc cctgcagccc aagcgggtag aaatccactg gacgacggac
      601 aactggcgca gcaatcaggt tacgccctgc ttcttctata ggatgcactg ggacaagtgg
      661 cgtaggagcc gcgcccgaaa cccgaatcga tacgatacaa gcatctggat cagccagatc
      721 aacgccgacg atgcatttcg tgttcaatat gcgatcggtt gcaaaacgcc gagccagacg
      781 ttctgggata acaacttcgg ccacaactac gtggcccgtc gccgccggtt gaagatcctc
      841 acgctgaacc tgcactgcta tcaagaggaa aaccaggatg cgaagttcag tcagattgcc
      901 agggccatcg acgacctcga cgtcgatgtc gtctgtctgc aggaggtggg cgagcaatgg
      961 gggaatggcg ccggggactg ggagtccaac gcggccaaga tcattcgcga ccggctgcgc
     1021 caacactatc atctgcagac cgattggtcg catatcggct ttgaccgata ccgggaaggc
     1081 atcgccgtcc tgagcaggta cgatttcctc atgaccgacg ccggctatgt gtcttccagc
     1141 caagatgtcc atagcatcga ttcccgcaag gtcgtgatgg tccagctcca cgtgccatac
     1201 atgggcgtgg taaacgtgtt ttccgcccat ctgagttggc caagcggcgg ttttttcgag
     1261 cagttcgatc ggctgcgcgc gtgggcgaac cacaagcatg gcaaccacct cgcagccact
     1321 tttctgtgtg gagacttcaa catcaaaacc ggctcggagg gatatcaggc ggtcgtgcgg
     1381 acccgcgagt atgaagatca atacctggcg gcgagttcac cgaacgcgtt cgaaaagatc
     1441 ttccgacagc aatcgtcgga catcgaccgc cgcttggcca aggatggtcg catcgatttc
     1501 gttttcatgc aaaagcacag ctcgctgcag gccgtcgcgg ccagggaact gttcacgaat
     1561 gacgatgatt acggtcgcgt ttccgatcac accggctact gcgtcgaatt cgagccgaac
     1621 tggtgattcc gctatgcgcg gtcctcggaa gccttgtcag acccgcgtgc aaaaagatgt
     1681 ggtcgggtgg ccgagaccgt cacaccacac cgcaaatacg gataggcgtc cgcactttcg
     1741 caatctcccg cccggagcac gcgccacgtc ggcgccctta catgccccgc ccgcacccag
     1801 cggtcggcct aattcaacaa ccgcgaggtc gcgcaggcgc ggtgacggcg gtcattccga
     1861 gccggacgct cgccgtcgcg ctgcacgacg tcgagccgag ccacgagccc cataatgctg
     1921 catcgtgagc ggcaaaacgt gtgctctccc tcctacggtc tcgcatacgt gcctcggaca
     1981 cgctccgagt agtaaagaaa gttacacaat gccattcgcc gaacgatacg catttcctat
     2041 cgacgccgag atcggcacga gcattccgcg tacgaacgac ttcaaggatt tgttctacgt
     2101 acgctgggga agcatccgat tcgacgtact ctggggagac gagctcaatc tgaaggtcgt
     2161 gttgaatata tatcgtcctg ataacatctg cgagcgattc atcgtcgaca cggacgctta
     2221 ccacgtacag tggaaccgtc acaagcgtgt caccagggat ttctatctcc acccgttccc
     2281 gctcaaactg gggcccgtca ggcgggtcac gttctcg
//
LOCUS       DATMGX010000613         4122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_438586, whole genome shotgun sequence.
ACCESSION   DATMGX010000613 DATMGX010000000
VERSION     DATMGX010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438586"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1628
                     /locus_tag="VNR65_09995"
     CDS             117..1628
                     /locus_tag="VNR65_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HWJ19044.1"
                     /translation="MKDTDYPGIATDAVALTDTIDGIDDGPDGYARLEAAEALWTGLC
                     KGCAAGWHDLSALWFDNGRMHMALSEPQTRRRAIVSFEANYGAYPAVGRHHTPAMRLE
                     RAMRDLYGTNPIGLPDGRAWLDHGVWPSRPAEKGYSFLPVEGVGLHQIPVGPVHAGII
                     EPGHFRFTANGETVARLEERLGYVHKGIEALCRGASIDQAGKVAARISGDSTVAYSFA
                     FSRAVEKALGWEIPVRAHMLRGVMAELERLANHVSDVGAICNDASVIALQARCAIIRE
                     EILRINVGCFGHRMMMDCIVPGGVACDVDKDCSAAILALMDTIEASFVRVARVYDQSP
                     SLQNRTVGTGIAESEFVREFAAGGYIGRAAGRAFDARAAFAYPPYDSVTFDIPTRTKS
                     DVDSRVWVRIDEIGQSISIVRQLLAKLETGPVFSPPPPFVPGTGAALIEAFRGDLFLS
                     VRLEVDGGVAHLHARDASWFQWPLLETAIKGNIVADFPLCNKSFNCSYSGHDL"
     gene            1637..2161
                     /gene="nuoB"
                     /locus_tag="VNR65_10000"
     CDS             1637..2161
                     /gene="nuoB"
                     /locus_tag="VNR65_10000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HWJ19045.1"
                     /translation="MHILLLDALLKGPATTAAPKPPEAMLADLAKELGERARSRLGRS
                     LAIREVDAGSCNGCELEINALNNPIYDVERFGLKFVASPRHADVLLVTGPVTWNMREA
                     LERTYAATPDPKWVVAVGDCGANCGVFAGSYACVGPVSSVIPVDLTVSGCPPQPVELL
                     KGLLGLMVETNKST"
     gene            complement(2314..2670)
                     /locus_tag="VNR65_10005"
     CDS             complement(2314..2670)
                     /locus_tag="VNR65_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19046.1"
                     /translation="MSNSSKTLTEVRRQLVTGIILMLAGVIWFSVILYGIRPDQILPT
                     NENVHTALDALKAQLGLPFKQWDIDAVRLVVTKSIVLFAANVLFIITRYGGLTLFFLG
                     GITIFDAISKAPRASH"
     gene            3351..>4122
                     /locus_tag="VNR65_10010"
     CDS             3351..>4122
                     /locus_tag="VNR65_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HWJ19047.1"
                     /translation="MHGLKLSRLLCIFLLAFAFFAARAEEAPPNPHAAQAKPIGGDPA
                     SHLPPDSVTQHTITLGGQQLTYKAMAGTLPLFGAKGEIAAKIFYVSYVLDNAPARPVT
                     FAFNGGPGASAAFLHMGAIGPRVVPFTENGAEPVLPVELTDNADSWLAFTDLVFVDPV
                     GTGYSRAATGGEDGEHAFWGVDKDADSITEFVNLYLARNGRELAPVYLAGESYGGFRA
                     ALLSDRLLADGVQVKGAIMISPALEFSMIRDGDLTLLPL"
BASE COUNT          829 a         1201 c         1191 g          901 t
ORIGIN      
        1 caggtcaatg cctccccagc ccccttgatc gtccatctct ccctggttct cattgcgggg
       61 ctctatctgc cggcggcttt ggtggccggg ttccaccgcg ccgcggagtt gctcggatga
      121 aagacacaga ttacccagga atagcgacgg acgccgtcgc gcttaccgat acaatagacg
      181 gtatcgatga cgggccggac ggctatgcgc gccttgaggc ggcggaagcg ctctggacgg
      241 ggctttgcaa agggtgcgcc gcaggctggc acgacctcag cgcactttgg ttcgacaatg
      301 gccgaatgca catggctctg tcagaacccc aaactcggag gcgagccatc gtctcatttg
      361 aggcgaacta tggcgcttac cccgcggttg ggcgtcatca tacgcccgcg atgcggcttg
      421 aacgggcgat gcgcgatctt tacggcacga atcccatcgg gcttcccgat ggccgcgcgt
      481 ggctagatca tggcgtgtgg ccaagccggc cagccgaaaa agggtacagc ttcctgcccg
      541 tcgaaggcgt cggcttgcat caaattcctg tgggcccggt tcacgcgggg attatcgagc
      601 caggacattt tcggtttaca gccaacggcg agacagtcgc ccgcctcgaa gagcggctcg
      661 gctatgtgca caagggcata gaggcgctct gcaggggcgc ttctatcgat caagcaggga
      721 aagtcgccgc gcgcatctcc ggcgacagca cagtcgccta tagtttcgcg ttctcgcgag
      781 ccgtggaaaa agcgctgggc tgggagattc cggtgcgcgc ccacatgctg cgtggcgtca
      841 tggccgagtt ggagcgtctt gcgaaccatg tgagcgatgt gggcgcgatc tgcaacgacg
      901 ccagcgtcat cgcccttcag gcccggtgcg cgataatccg cgaggaaata ctgcgcataa
      961 acgtcggatg ctttggccat cgcatgatga tggattgcat cgttccgggt ggagtggcct
     1021 gcgatgtgga caaggattgc agcgccgcaa tccttgcgct catggacacc attgaagcca
     1081 gcttcgttcg agttgcgcgc gtctacgatc agtcgccctc tttacaaaac cgcacagtgg
     1141 gtacgggcat cgcagagagc gagttcgtcc gggagttcgc cgccggcggc tatatcggca
     1201 gggccgcggg ccgcgccttc gacgcacgag cagcattcgc ttacccgccc tacgacagcg
     1261 tcacattcga tataccgacg cgcacgaaaa gcgatgtcga ctcgcgtgtt tgggtccgta
     1321 tcgacgaaat cggccagagc atctcgatcg tgcgccaact tctggcgaag cttgaaacag
     1381 gaccggtgtt ctcgccgccg ccaccgttcg tccccggcac aggagccgcc ctaatcgaag
     1441 cgtttcgcgg cgatctgttt ctctcggttc ggctcgaagt ggacgggggg gtcgcccatc
     1501 ttcatgcccg cgacgcatcc tggtttcagt ggcccctcct cgagaccgct atcaaaggca
     1561 acatcgtcgc cgactttcct ttgtgtaata aatcgttcaa ctgctcctat tcgggccatg
     1621 atttgtaggg ccgtccatgc atatcctcct tctcgacgcg cttctcaaag gcccagcgac
     1681 aaccgctgcc ccaaaacccc ccgaggcgat gcttgcggac ctggcgaagg agctgggtga
     1741 gcgcgcgcgc agtaggctcg gacgttccct cgcaatccgc gaggttgacg ccggctcgtg
     1801 caacggctgc gagcttgaaa tcaacgcgct caacaacccc atttacgatg tggagcgctt
     1861 cgggctgaaa ttcgtcgcgt cgccccggca tgctgacgtg ctgctggtaa ctggccccgt
     1921 cacatggaac atgcgcgagg cgctggagcg cacatacgcc gcaactcccg atccgaagtg
     1981 ggttgtagcc gttggtgact gcggggcaaa ttgcggggta tttgcgggaa gctacgcttg
     2041 cgttggcccg gtgtcttccg tgattcctgt ggacctgact gtgagtggct gtcctcccca
     2101 gcctgtggaa cttcttaagg gtctgctcgg gctcatggtg gaaaccaaca agtcaactta
     2161 gatgaagctg cttgccagca gcggttgctc atttgcaata agttgacgcg aatggcttgc
     2221 gctccttaga aacgctcagc cgcgctaagc gggcgtatat ggatgggttc ggggcataag
     2281 gacgtacagc aataccgaat gatcttcctg taactaatgg ctagctcttg gagccttgga
     2341 aatagcgtcg aaaatagtga tgccacccag gaaaaacaga gtgagtcctc catatctagt
     2401 gataatgaac aagacattgg cagcgaacaa gacaatggac ttggtgacca ccaaccttac
     2461 agcatcaata tcccattgct tgaatgggag gcccaattgc gctttcaaag catcgagtgc
     2521 cgtgtgtaca ttttcatttg ttggtaagat ctggtccggc cgtattccat agaggatgac
     2581 actgaaccaa atgacgcctg cgagcatgag aattatgcct gttacgagtt gtcttcgaac
     2641 ttctgttagc gtctttgatg aattgctcat gataagtctc taagacaatg tcgcctttct
     2701 aagacaagtc cgccaggacc acttatattt gtaatgacct ggacctcgcc cccggtcgac
     2761 tttgtcattt cgatcagcga tcaagtgacg ggtgcttctt gctcttcact caacaggaaa
     2821 tgcgaagtgt cgatcccgga acccgctctc agctggtaca aagatctgtg ttcaggctct
     2881 tcctcaacgg cgagcctcat gggtttaaat caatggcgaa gctaagttct cgcgttttgc
     2941 aattgagagt tgaacgcctc aaaatgcgac gcagcgttct gatatctgac tgggtaacga
     3001 cacctcacag cgaatcgccg agtgcccctc aatcggagat accattggtg acctgatcag
     3061 gtcactcata ggaggaacgt ccgccagcct ttgacggata tcgccgctct catgaagccg
     3121 cttgaagcgg aacttctcat cgccaagcgg gtgcatcgag caaaaatagc gaagtgaacg
     3181 gatccgcccg cttagcagaa gtttgaccta cgcctctgcg gcctccgtga ccggccaagg
     3241 attggaaatc tgagcctcgc ttgctatctc tttccatcaa gggaagtatc tcgatttcgc
     3301 tgactaagta tttcctgatt tccacctgcc cgggattgaa ggcagctgcc atgcatggac
     3361 tgaagttgag ccgtttgctt tgtatttttc ttctcgcgtt tgcattcttt gccgcgcgcg
     3421 ccgaagaggc gccccccaac cctcacgcgg ctcaagcaaa gccgatcggt ggcgatcccg
     3481 cctcccacct cccgccggat agcgtaacgc aacacaccat cacgctcggg gggcagcagc
     3541 taacctataa agcgatggca ggcactttac cgctgttcgg cgcaaaggga gagattgccg
     3601 cgaagatctt ctatgtttcc tacgtgctgg acaatgcacc ggctcggccg gtgacgttcg
     3661 cttttaacgg tggcccgggc gcttcggccg cctttctgca catgggcgcg attgggccgc
     3721 gcgtggtccc ctttacagaa aacggcgcag agccggtgct gcctgttgaa ctcacggaca
     3781 atgccgatag ctggcttgcc tttacggatc tggtcttcgt ggatcccgtc gggacgggct
     3841 acagccgggc tgcgaccggc ggcgaggatg gcgaacacgc cttctggggt gtcgacaagg
     3901 acgcggattc catcaccgaa ttcgtgaacc tctatctcgc gcgcaacggc cgcgaacttg
     3961 ctccggtcta tctggccggg gaaagctatg ggggatttcg cgccgccctt ctttctgacc
     4021 ggctcctcgc cgacggggtg caggtgaaag gcgcgatcat gatctcgccc gcacttgagt
     4081 tctccatgat acgcgacggt gatctgacgc ttctgcctct ca
//
LOCUS       DATMGX010000614         2421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_96917, whole genome shotgun sequence.
ACCESSION   DATMGX010000614 DATMGX010000000
VERSION     DATMGX010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96917"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VNR65_10015"
     CDS             complement(<1..680)
                     /locus_tag="VNR65_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ19048.1"
                     /translation="MSLGTPPERDEILRVEHLTMRFGGLVAVDGLSFSARRGDITAII
                     GPNGAGKTTVFNCITGFYKPTEGRIALAHGTGAAFEALPKVTFGGKQWVATPEGAVFL
                     LERMPGFEIARTAHVARTFQNIRLFAGMTVLENLLVAQHNVLNAASGYTIGGLLGLDS
                     YKRAESEAIERSAYWLGEIGLLDRADDPAGDLPYGAQRRLEIARAMCTAPVLLCLDEP
                     AAGLNPRES"
     gene            complement(680..2266)
                     /gene="livM"
                     /locus_tag="VNR65_10020"
     CDS             complement(680..2266)
                     /gene="livM"
                     /locus_tag="VNR65_10020"
                     /inference="COORDINATES: protein motif:HMM:NF008450.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HWJ19049.1"
                     /translation="MTDTAKPESAPVVHRMPGWLPTDALRDAAITFLIALALFGSLVG
                     LRADTVNNALEITTHFGKALLLSGIVAGLRLAVDLLVWRRVADGETIASLIPALIPVG
                     RALAGFGLVLGFVLYVFPTLRSDLIAWASGSFWWLGSIADLVAFACIAGFVVAAAMVL
                     SRVARIAFTRRTPVSGIAARLTTDFGREFPVALLVIAIALPLLITGASQRYTFDTATQ
                     ILIYIMLGWGLNVVVGLAGLLNLGYVAFYAVGAYTYALISTELGWSFWVCLPLAGMLA
                     VLSGIALTFPVLRLRGDYLAIVTLAFGEIIRIVILNWQSLTGGANGVNRIPKPTFFGL
                     SFDKTAHPSFHSFFGLEYNPVYRIIFLYYVILVLALITNFVTLRLRRLPIGRAWEALR
                     EDEIACRSVGINTVITKLTAFAIGSMFGGFAGAFFSARQGFISPESFTFMESAMVLAI
                     VVLGGLGSQLGVVIAAVVLVGGLERFRELQDYRMLIFGLSMVLVMIWRRHGLMAGRTP
                     SALLHEARSISANLVKERRG"
BASE COUNT          553 a          708 c          789 g          371 t
ORIGIN      
        1 gactcgcgtg gattgaggcc ggctgcgggt tcatcaaggc acagcaaaac cggcgctgtg
       61 cacatggcgc gcgcgatctc gagccgcctc tgcgcgccgt agggaaggtc gccagcggga
      121 tcgtcggcgc ggtccagaag gccgatctcg ccaagccaat aggccgagcg ctcgatcgct
      181 tcactttccg cccgcttgta gctatcaagg ccgagaaggc cgccaatggt gtagccggag
      241 gcggcattta agacgttatg ctgggcgacg agcaggtttt cgagcacggt cataccggca
      301 aacaagcgga tgttctggaa ggtgcgggcg acatgggcgg tgcgtgcaat ctcgaagccc
      361 ggcatgcgct ccaggagaaa gacggcgcct tcgggtgtcg cgacccattg ctttccgccg
      421 aatgtgacct tgggtagcgc ttcaaaggcc gcgcctgtgc catgggcaag ggcaatgcgt
      481 ccctcggtgg gcttatagaa accggtgatg cagttgaaga ccgtggtctt gccagcgcca
      541 ttcgggccga tgatcgcggt gatatcgccg cgccgcgcag agaaggagag cccgtcgacc
      601 gccacgaggc cgccgaagcg catggtgaga tgctccacgc gcaggatctc gtctctttcc
      661 ggaggcgtgc ccaggctcat cagccgcgtc tctccttcac caagttcgcc gaaatgcttc
      721 gtgcctcatg cagaagggcg gaaggcgtcc gccccgccat gagtccgtgc cgccgccaga
      781 tcatgacgag caccatggac aagccaaaaa tcagcatgcg gtaatcttgc agctcgcgga
      841 agcgctccag gccgccaacc agaaccaccg ctgcgataac gacgccgagc tggctgccga
      901 ggccgccaag cacgacgatt gcgagaacca ttgcggattc catgaatgtg aatgactcgg
      961 ggctgatgaa gccttgccgc gcggagaaga acgctcccgc gaaccctccg aacattgagc
     1021 cgatggcgaa ggcggtgagc ttcgtgatga cagtgttgat gccgaccgag cggcaggcga
     1081 tttcatcctc gcgcaaggct tcccaagccc gcccgatggg caggcggcgc aggcgcaacg
     1141 tcacaaagtt ggtgatgagc gccagaacga ggataacgta ataaaggaag atgatgcgat
     1201 agacgggatt gtactccagt ccgaaaaagc tgtgaaaact cggatgcgcc gtcttgtcaa
     1261 aggagagacc gaagaaggtc ggcttcggga tgcggttgac gccgtttgcc cctcctgtca
     1321 ggctttgcca gttcaggatg acgatgcgga tgatctcgcc aaaggccagc gtcacgatgg
     1381 cgaggtagtc acctcgcagg cgaagcacag gaaaggtaag ggcaatgccc gagagcaccg
     1441 ccaacatgcc ggcgagcggg agacacaccc agaaagacca gcccaactcg gttgagatca
     1501 gcgcataggt gtaggccccg actgcgtaga aggccacata accaagatta agcaatcccg
     1561 cgaggcccac gaccacgttc aggccccagc cgagcatgat gtaaataagg atctgggtcg
     1621 cggtgtcgaa tgtatagcgc tgcgaagcgc cggtgatgag gagcggcagg gcaatcgcaa
     1681 tgacgaggag cgcaaccgga aactcccgcc cgaaatccgt ggtgaggcgg gcggcgatcc
     1741 cgctcaccgg agttcgcctt gtaaaagcga tgcgagcaac tcgggagagg accatcgcag
     1801 cggccacgac gaagccggcg atgcaggcga aggcgacgag atccgcaatc gagccgagcc
     1861 accagaatga accgctcgcc catgcgataa ggtccgaccg cagcgtggga aagacgtaga
     1921 ggacaaagcc aagaacaagg ccgaagcctg ctaaggcgcg cccaaccggt atgagggcag
     1981 ggatcagaga cgcgatggtc tcgccatcgg caacgcgtcg ccagacgagg aggtcgacag
     2041 cgagccggag gcccgcgaca atgcccgaaa gaaggagggc cttgccgaaa tgcgtcgtga
     2101 tttcaagcgc attgttcacc gtatcggctc tgagtccgac gagcgatccg aacaaggcca
     2161 gcgcaatcag gaaggtgatg gcggcgtccc gcagggcgtc cgtggggagc cacccgggca
     2221 tgcgatggac gacaggcgcc gattcgggct tggccgtgtc cgtcatcgct cagacctttt
     2281 ccacttccgg ccgtccgagg agtcccgatg gcatgaagat gagaacgacg acgaggatgg
     2341 agaacgccgc cacatccttg tattgtgccg tgaaatagcc tgcccagaaa acttcgatca
     2401 gcccgatggc gaggccgccc a
//
LOCUS       DATMGX010000615         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_203266, whole genome shotgun sequence.
ACCESSION   DATMGX010000615 DATMGX010000000
VERSION     DATMGX010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203266"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VNR65_10025"
     CDS             complement(<1..530)
                     /locus_tag="VNR65_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19050.1"
                     /translation="MSIRPVVSCVGAGAIAVCFAATPPDVAHAQVNVLKECGAQFQAA
                     KTENALKGQGWQDFLRACRVRLAEQPPPAAAPAASDAPNAQSNILKECGTQYQAAKAA
                     NKLQGQGWQDFLKICRAHVAEVPAPAKTPAAAEPAPTAAPASPSKPAAEAVAPAVAPT
                     TVPDVVSTKLVSEGKAA"
     gene            complement(643..1632)
                     /locus_tag="VNR65_10030"
     CDS             complement(643..1632)
                     /locus_tag="VNR65_10030"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ19051.1"
                     /translation="MATIIRRREIAAHPYEEIEHSVAFAYNLAGEKYGVYADGDPGEL
                     NAFRGQHAYCDQRIWALLDEKLRAVRSSGARSIRILDLGCGPGAWLRRIATRAWALGF
                     TSIAARGIDLADAQVREARTQSRKLTELGGVSLTFEVGDICGQLSEADGCVDLCLCLY
                     GVLNHIPTDRLPAVLSEAARVTSGQFIMTARAIGSTPTICVDALEQARWFWRNNRTNR
                     LEIELQNGRYVSFDYKLFRAEELRALAAPHFELNDLKGLDLFHGRFAGDRRWNSPTST
                     VDAQFVHELDRLEEFYCRDAKFIDHATHLLLVATPRKHSELNPEELPTFGFAD"
     gene            complement(1725..1991)
                     /locus_tag="VNR65_10035"
     CDS             complement(1725..1991)
                     /locus_tag="VNR65_10035"
                     /inference="COORDINATES: protein motif:HMM:NF005480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWJ19052.1"
                     /translation="MLETIQRLIDEKGSLSIAARNLPRDADLYRAGLTPFSAIQVMLA
                     LEKEFNIEFPKWMLNRQSMSSINAIVFRLCELQEQQRKMPAHAA"
BASE COUNT          490 a          616 c          656 g          489 t
ORIGIN      
        1 gctgctttgc cctcgctcac cagtttggtg gaaacaacgt caggaacggt cgtcggcgcg
       61 accgcgggcg cgacagcttc cgcggcgggt ttcgatggac tggcgggagc ggcggtcggg
      121 gccggctccg cggctgcggg agtctttgcc ggcgctggta cttcggcgac atgcgcgcgg
      181 caaatcttca ggaagtcctg ccacccctgg ccttgtagtt tatttgcggc cttagccgct
      241 tgatattgcg tgccgcactc cttcaaaata ttcgactgtg catttggtgc gtccgatgcc
      301 gcaggagctg ctgctggcgg cggttgctcg gcgagacgaa cgcggcaagc ccgtaggaaa
      361 tcctgccagc cctggccttt caaagcgttc tcagttttgg cggcttgaaa ttgcgcgccg
      421 cactctttca agacattgac ctgcgcatgc gctacgtcag gaggggtggc tgcaaaacac
      481 acggcgatgg cgccggcccc aacgcaagag acaacgggac ggatggacat aagatttctc
      541 ccataagtga caggacctag acaagccgaa cctgatggag cgaatgcgac attctctgcg
      601 cctcgccgat agagcgttat ccgtacgaat cgaacctggc gctcagtcag cgaatccgaa
      661 ggttggcagc tcttcgggat taagctcact gtgctttcga ggggtggcga cgagcagaag
      721 atgcgtagcg tggtcgatga acttcgcatc tctgcagtaa aattcttcga gccggtcgag
      781 ctcgtggacg aattgcgcgt cgaccgtcga ggtaggcgaa ttccagcggc gatcgccagc
      841 aaatcgacca tggaagaggt cgagcccctt gaggtcattc aactcaaaat ggggcgcggc
      901 aagggcccgt aactcctccg cccgaaagag cttgtaatcg aacgaaacgt agcggccatt
      961 ctgaagctca atctcgagcc ggttggtccg gttgttccgc cagaaccagc gtgcctgttc
     1021 aagcgcatcc acacaaattg tcggcgtgct gccaatggcc cgcgcggtca taatgaattg
     1081 gccgcttgta actcgtgctg cttcggagag aacagcagga aggcggtccg tcggaatgtg
     1141 atttagaact ccgtagagac aaaggcagag atcaacgcag ccgtcggcct cagaaagctg
     1201 cccgcaaata tcgcccacct cgaacgttaa actaactcca ccaagttctg tcagcttgcg
     1261 gctctgggta cgcgcttcgc gcacttgcgc gtcagcgagg tcaattccac gcgcggcgat
     1321 cgaggtaaat ccgagggccc atgcgcgcgt tgcgatacgg cgaagccagg cgccaggtcc
     1381 gcaccctaag tcgaggatcc gaatcgatcg agcgccggaa gaacgcactg cgcgcagttt
     1441 ctcatcgagg agagcccaga tgcgctgatc gcaataggcg tgttgcccac ggaatgcatt
     1501 gagctcgccc ggatcgccat cagcgtagac tccatacttc tcaccggcaa gattgtaggc
     1561 aaacgcgaca gaatgctcga tttcttcgta aggatgagca gcgatttcac gtcttcggat
     1621 tattgtggcc atctcttttc cacttctggc ttgtttagct aagtgacaac gcgatccgac
     1681 tctgtctgcc tcagtttgct atggggaagg ttacgcgtga gaattcatgc cgcatgggcg
     1741 ggcattttcc tctgttgctc ttgcaattca cagaggcgga agacgattgc attgatggac
     1801 gacatgcttt gacgattgag catccacttg gggaattcga tgttgaattc tttttcaagc
     1861 gcgagcatca cttgaatggc cgagaacggc gtcaggcctg ctcgatagag atctgcatcg
     1921 cgcggaagat ttcgcgccgc gattgacagg cttcccttct catcgatcag ccgttggatg
     1981 gtttcgagca tggctttcat gttccaatga agctgatgga acatatccgc aaacgacata
     2041 aaggctccat tcggaaaatg ggcgccgcac ataaaagtta tataaaggcg aagatccaat
     2101 tcggctttat gcggcgctta tacacgcgag caaaaatctg aatgggacct ttgtgccgct
     2161 tcgatttacg ctgactcgat agcaccctat cgcagcgggt gatgctcaat cccgttgatg
     2221 tgagtccaag gctccggaac ggtcgaagct g
//
LOCUS       DATMGX010000616         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_423753, whole genome shotgun sequence.
ACCESSION   DATMGX010000616 DATMGX010000000
VERSION     DATMGX010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423753"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..980
                     /locus_tag="VNR65_10040"
     CDS             21..980
                     /locus_tag="VNR65_10040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWJ19053.1"
                     /translation="MSDLLFDQDVAYANFESPITTQPLIKEVIGDREPPTECCSRAQF
                     DVLKGHKGRVFDVLNTSNNHAFDMGVEGLETTLGVFAEEGILETGTNRNAQQSSQGRL
                     LVKDGIKLGFVSSTFGLNGRPVPPEDAYRINAARLLSKHVDPELDLLKQQIDWCKREG
                     CDFIIASLHWGFEFEFFPRKRQVEAARELVEYGADTILCHHPHVIQPVEYYRPHRDPA
                     RAAVIAYSLGSLTWGFTAPHLVLSLLLNLKLTKGSSQGQERTYIEDVCVTPIFRSVVN
                     NAEGVETRLEKLADHLDGRSTRHSADYIAQIDSYARLVLGKTI"
     gene            995..1948
                     /gene="serB"
                     /locus_tag="VNR65_10045"
     CDS             995..1948
                     /gene="serB"
                     /locus_tag="VNR65_10045"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HWJ19054.1"
                     /translation="MFVIGLKLVMEIAITVTALPSQDKLLLDVAAALQRELNGALEFR
                     WLAQGEAIDLILPSPDGAPETPQPADISNADLAYIKEMVAHAAGNRPLDWCVQRTVGR
                     KKRMLIADMDSTTIGQESIDEMAELKGIRPEIAAMTERSMRGELEFESGLRERMRLLR
                     GMSEADLQRVLGRLTLNPGARTLVQTMKAHGAHTVLVSGGFTFFTSAVAERAGFAANR
                     GNVLLWDDGRLAGAAEPIQGREAKLTALNEEAAAHSILPADVIAVGDGANDLAMLKAA
                     GLSIAYRAKPIVAAEADAQVRYTDLTALLYFQGYMRDSFAA"
     gene            complement(1945..>2127)
                     /locus_tag="VNR65_10050"
     CDS             complement(1945..>2127)
                     /locus_tag="VNR65_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19055.1"
                     /translation="RFLFGAFTIADAMYAPVATRFRTYGIDLGQFGDDGTAADYAETL
                     LAMPEIQEWGEGAARG"
BASE COUNT          457 a          603 c          622 g          445 t
ORIGIN      
        1 aagacatcct ttttgagaat atttctgacc tgttgttcga tcaggatgtt gcttacgcca
       61 attttgaatc tccgataacc actcaacccc tgataaagga agtgatcggt gaccgcgaac
      121 cgcccactga atgctgctca cgagcccaat ttgacgtctt gaagggacat aaaggcagag
      181 tcttcgatgt gttgaatacg tcgaataacc acgcctttga tatgggcgtt gaaggccttg
      241 aaacgacctt aggcgtattt gccgaggaag gcattctgga aaccggcaca aatagaaacg
      301 cacaacagag cagccaagga agacttctcg ttaaggacgg cataaagctt ggctttgtct
      361 cctcgacatt cgggctcaat ggccgtcctg tacctcctga ggatgcctac cggattaatg
      421 cggccaggct cctctcgaaa catgtcgatc ccgagctaga ccttctcaag cagcagatcg
      481 attggtgcaa gcgcgaggga tgcgatttta ttattgcttc actgcattgg ggcttcgagt
      541 ttgaattctt tccgcgcaag cggcaggttg aagcggctcg tgaattggtt gaatacggcg
      601 ccgatacgat cttatgccat cacccccatg tcatacagcc ggttgaatat taccgtcccc
      661 accgagatcc cgcgcgggcg gcggtcattg cctattccct gggaagtctt acctggggtt
      721 tcacggctcc gcatctcgtc ttgagccttc tcttgaatct caagctgacc aaaggatctt
      781 cgcagggtca ggaacgaacg tacattgagg acgtgtgcgt tacgccgatt tttcggagtg
      841 tcgtcaacaa tgccgaaggc gttgaaactc gtctggagaa gcttgccgat cacctggatg
      901 gccgcagcac aagacacagc gcagattaca ttgctcagat agactcctac gcgcggctgg
      961 ttctaggcaa gactatctag ttcggcgagg gattttgttc gtcatcggat tgaagctcgt
     1021 catggaaatc gcgatcaccg ttaccgccct cccttcgcag gacaagttgc tgctcgatgt
     1081 cgccgccgcg ctgcaacgcg aattgaacgg ggcgctggaa ttccgctggc tggcccaagg
     1141 cgaggccatt gatttgatcc ttccatcgcc tgacggcgcg ccggaaacgc cgcagcccgc
     1201 ggacattagc aatgcggacc tcgcctacat caaggagatg gttgcccacg ccgctgggaa
     1261 caggcccctg gactggtgcg tgcaaaggac ggtcggccgc aagaaacgca tgctcatcgc
     1321 cgacatggat tccaccacca tcggccagga gagcatcgac gagatggcgg agctgaaagg
     1381 catccggcct gaaatcgcgg caatgacgga gcggtcaatg cgcggggagc tggagttcga
     1441 gtccggcttg cgcgagcgca tgcggctgct tcggggaatg tcggaggccg atttgcaacg
     1501 cgtgctgggg cgtctcacgc tcaatccggg cgcgcgcacg ctggtacaaa caatgaaggc
     1561 gcatggcgcg catacggtgc tggtttcggg cggcttcacc tttttcacga gcgccgtcgc
     1621 cgagcgagcc ggattcgctg cgaacagagg caacgtcctc ttatgggacg acggccgcct
     1681 tgcgggcgcc gcggagccta ttcaagggcg ggaagccaag cttaccgcat tgaatgaaga
     1741 ggccgccgcg catagcattt tgcccgctga tgtgatcgcg gttggcgacg gcgcaaacga
     1801 cctcgccatg ctgaaggcgg ctgggctcag cattgcctat cgggcaaagc ctatcgtcgc
     1861 ggccgaagcg gatgcgcaag tgcgctacac cgacctcacc gcgcttctct atttccaggg
     1921 ctacatgcgc gacagtttcg cggcttaacc gcgggctgcg ccctctcccc attcctgtat
     1981 ctcgggcatg gccagaagcg tctccgcata atcggcagct gtgccatcgt caccgaactg
     2041 gccgaggtcg atgccatagg ttcggaaacg cgtcgcgact ggcgcgtaca tggcatcagc
     2101 gatggtgaaa gcgccgaaca gaaaacg
//
LOCUS       DATMGX010000617         2114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_133617, whole genome shotgun sequence.
ACCESSION   DATMGX010000617 DATMGX010000000
VERSION     DATMGX010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133617"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VNR65_10055"
     CDS             <1..669
                     /locus_tag="VNR65_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19056.1"
                     /translation="ANSIPTRAFDTESPRGTLKRFHPEITEIVDMTTTSDQMKIFDTL
                     NAATPSVTVIDVRAGLMSPALASLRDIGFLDAAKSGQITFAAFHLLGPSIASLDEIAE
                     TANFMQGAKYFLVKNFINDTNFFEWDKTTYNFYFRKLQDAIELSIPKLNEMASEQVEV
                     ASVPFLKFVANKGVDDKPADYSFVLRGYVRHWLMNVWREFDRIKLTDLVGSSKSARIE
                     RKAS"
     gene            complement(1013..1408)
                     /locus_tag="VNR65_10060"
     CDS             complement(1013..1408)
                     /locus_tag="VNR65_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HWJ19057.1"
                     /translation="MTTDLRYLVYTSMLTAALWIPYVICQVMTNGPLQPKNYVDPALP
                     RPVPAWGQRAHRAYLNAIEVFAPFAALVVVTQLAGKANAMTAFWSASFFYLRLAHAIV
                     YWAGLPYLRTILFTLAFVAEAGIFWEVIK"
     gene            complement(1898..>2114)
                     /locus_tag="VNR65_10065"
     CDS             complement(1898..>2114)
                     /locus_tag="VNR65_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19058.1"
                     /translation="SASKRFATKGFLMNQTHHGRGTEMWGRPAASLKLQHHWALASRT
                     VLLNPFSIAGRERYRTLSHRWKPMGER"
BASE COUNT          481 a          606 c          600 g          427 t
ORIGIN      
        1 gcaaacagca tacccacccg ggccttcgat accgaatctc cgcgcggtac gctgaagcgc
       61 tttcatcctg aaattaccga aatcgttgat atgacgacga cttcggatca gatgaagatt
      121 ttcgacacgc tgaatgcagc aaccccctct gtcaccgtga tcgatgtccg cgccggtctg
      181 atgtcgccgg cactcgcctc gctgcgcgac attggatttc tcgatgccgc gaagtcgggc
      241 cagatcacct ttgcagcatt tcacctgctc ggcccctcaa tcgcctcgct ggacgagatc
      301 gcggagaccg cgaactttat gcaaggcgcg aaatattttc tcgtaaagaa cttcattaat
      361 gatactaact ttttcgagtg ggataagacg acctataatt tctatttccg caagctccag
      421 gacgccatcg agctcagcat ccctaaactc aacgaaatgg cgtctgagca ggtcgaagtc
      481 gcttcggtgc cgttcctcaa attcgtcgcc aacaaaggtg tggatgataa gcccgcagac
      541 tattcatttg tgttgcgcgg ctatgtccgc cattggctga tgaacgtctg gagggaattc
      601 gaccgtatca aactaaccga tcttgtcggc tccagcaaaa gcgcgcgcat agagagaaaa
      661 gctagctaaa cgttatttgg ctggaccgcg agccgttcct tcaatcttgt gtttgcgcgc
      721 tagtcctcga ccagacggcg ggcatttccg cacgacgaag ggtcgaatgg ttctgctatc
      781 tgcggcaggg cgagagtccg gtaatggcac gtttgagaca tgccggctga cactgagaat
      841 gtctgcgtgt tggggaagac cgaaagttgc cgaccgccgg tcagaacgac gtatgcccca
      901 caacggactc gccattgtgt cgatcaaatt ccaagccggc cgcgcggcag cgccacttgg
      961 tggctccgcg cgtcccttgt tcgatcatga ccccggatct tccgggagaa aactacttga
     1021 tgacttccca gaaaatccca gcctcggcaa cgaatgccaa agtgaacagg atggttcgaa
     1081 ggtagggaag cccggcccag taaacgatgg cgtgtgcaag cctcaagtag aagaagctcg
     1141 cggaccagaa cgcggtcatc gcgttcgcct tcccggccaa ttgtgtgacg acaacgagtg
     1201 ccgcgaaggg cgcaaaaacc tcgatcgcgt tcagataggc ccgatgcgcg cgctgccccc
     1261 atgcaggcac cggccgcggc aaggcggggt cgacatagtt cttcggctgc agcggcccgt
     1321 tggtcatcac ctgacagatg acatagggaa tccacagtgc ggcggtcagc atcgacgtat
     1381 aaacgagata tctgaggtcg gttgtcacct tagcctcctg agatggggtg cctcagttcg
     1441 cggttgcgac ctgatcggcc tctatagacg ttcgagacgg agccaaatcg acacggcctg
     1501 aacatttgtt tgcgaggcaa aagaagccgg tcctggcagc accgcaaaaa cggtcgccat
     1561 cgatctggca atgagccagc cgtggccgct tcaaggtccc ggcacttcag cgaaatgaag
     1621 ggccagcacc gaagctgtcc gatcgccgac gcagacccga agtgaccggc ggccggtcag
     1681 gacgacgtga ttgaccgatg tctcttcatg gcacgaagct gccagtccgg gatgtcagct
     1741 gtctggtcgc tgtcgggggt aaaccggaca tggcgcggac agcctatttc ggtggcgatt
     1801 gacccgaagc ggtcgtggcc acaatattag cgctcgccac gctgtttgag tcggggaccg
     1861 aacagacacg caaaggatca cgagctagtt tgaaacatca ccgctcgccc atcggcttcc
     1921 atcggtgact gagagtccgg tagcgctccc gcccagcaat ggagaagggg tttagcaaaa
     1981 ccgtacggga tgccagcgcc caatgatgct ggagcttcaa cgaagcggca ggacgccccc
     2041 acatttcagt gcctcgaccg tgatgggtct gattcattaa aaagcccttg gtagcgaacc
     2101 ttttggacgc gctc
//
LOCUS       DATMGX010000618         3115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_33388, whole genome shotgun sequence.
ACCESSION   DATMGX010000618 DATMGX010000000
VERSION     DATMGX010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33388"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VNR65_10070"
     CDS             <1..502
                     /locus_tag="VNR65_10070"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWJ19059.1"
                     /translation="DGQDVLAKYKIDAYVDHAAYQKGQRLDPTQIDWSSDAPLRYFYV
                     QNAGEDNPLGYAKINFASPHGVYMHDTPGQSIFSKTFRAESSGCVRVQNVHQLVAWLL
                     EDNGWSVQQVLRMKQTGERLTVRLKKRVPLYWTYITAWATPDSTVQFRNDIYRKDGLE
                     AIASAY"
     gene            complement(669..1070)
                     /locus_tag="VNR65_10075"
     CDS             complement(669..1070)
                     /locus_tag="VNR65_10075"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HWJ19060.1"
                     /translation="MMHADPRHLFVYGTLMRASRSPYAQLLRTRAQFVGEAWTPGRLY
                     HLGRFPGAVFGDDCTNRVHGELFLLRGNTLLAALDAYEGCGGQGTKPDLFYRDVVEVR
                     LARGGRIPAWVYPFAGTVRGRSHIASGRFPL"
     gene            complement(1067..2041)
                     /locus_tag="VNR65_10080"
     CDS             complement(1067..2041)
                     /locus_tag="VNR65_10080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HWJ19061.1"
                     /translation="MKAVLCKSLDGPDALEVGDLPASEPGAGEVAIRVSAVALNFFDT
                     LIVRGRYQTKPELPFSPGGELAGTVARLGPEVEGLSVGQRVLAYIGYNGCREEAIAPA
                     GRVVALPDGISDEAAASIPIAYGTALHGLEDRGHLWPGRTVLVLGASGGAGLAAVEVA
                     ACLNAEVVAAGTSDEKLKICAERGAAQLLNLTGINVKEAVRALNGGKGPDLIYDCIGG
                     PYAEPALRGIAWGGRYLVVGFAAGDIPKIPLNLVLLKGCEIAGVFWGRHVASNLPGFR
                     DQIARIIQWCVEGKIRPHIDRVFSLAETADALRAMEARQIKGKVVVKP"
     gene            2089..2598
                     /locus_tag="VNR65_10085"
     CDS             2089..2598
                     /locus_tag="VNR65_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWJ19062.1"
                     /translation="MRGYFAIGAERISKPMNMGNLLRSAHAFGAKFFFTIGAHPRAFD
                     AKSDTSKAVHHLPVYHWANADEIILPFRCKLVGVELIEGATDLPSFRHPLHAAYVLGP
                     ELGVLSEGLLSRCDHVIKIPTAFCINVAMAGAIVMYDRVRSLGSFPDRMEAPAPHLRA
                     RIDKLPTDR"
     gene            2675..>3115
                     /locus_tag="VNR65_10090"
     CDS             2675..>3115
                     /locus_tag="VNR65_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19063.1"
                     /translation="MRGGLIIPASFLILLAPCALAQQSDIEQVTPQPSQQPKAAPAKP
                     KPKPAPAKKPVPVAAPEVKARLLERIKDWTVYIYEGAEGRICFAASAPTDMQPKTAKR
                     TPVIFYVTTWQKDGVHTEVSVRQGYAMKANAVATVTVGGQNFSLS"
BASE COUNT          657 a          989 c          907 g          562 t
ORIGIN      
        1 cgacggtcag gacgtgctcg cgaaatacaa gatcgacgct tacgttgacc acgcagccta
       61 tcagaaaggt cagcggctcg atccgacgca aatcgattgg tcatcggacg caccgcttcg
      121 ttatttctat gtgcagaatg cgggggagga taacccgctc ggctacgcca agatcaattt
      181 cgcgagcccg cacggcgttt atatgcacga cactcccggg caaagcattt tctcgaagac
      241 cttccgggcg gaaagctcgg gctgtgtgcg cgtgcaaaac gtccaccagc ttgtcgcgtg
      301 gcttttggaa gataacggct ggtcggttca gcaggttttg cgcatgaagc aaaccggtga
      361 gcggctgacc gtgcggctga agaaacgggt gcccctctac tggacgtata tcaccgcctg
      421 ggcaacgccc gatagcaccg ttcagttccg gaacgatatc taccggaagg atggcctgga
      481 ggctatcgcc tcagcctact gacgcatgtt gcgatgagct aaatccgatg tcatggccgg
      541 gtaagcgatg tcatggccgg gcttgacccg gccatcagcc tataatccaa aaagatggga
      601 atttgttatc ctggatagcc gcctcggagg gcggccatga caaaaacacg ctctgaagcg
      661 agccggattt ataaaggaaa acggcctgac gcgatgtgcg aacggccgcg aaccgtaccg
      721 gcgaacgggt aaacccaggc ggggatcctc cccccgcgcg cgagccgcac ctccaccaca
      781 tcgcgataaa agaggtcggg ctttgtgcct tgcccgccgc agccttcata cgcgtccagc
      841 gccgcgagca gggtattgcc gcgcaggagg aaaagctcgc catgcaccct gttcgtgcag
      901 tcatcgccga acaccgcacc gggaaagcgg ccgagatgat agagccgccc cggcgtccat
      961 gcttcgccca cgaactgcgc ccgcgttctc agaagctgcg catagggact gcggctcgcc
     1021 cgcataagcg tgccgtacac aaacaaatgc cgcggatcgg cgtgcatcat ggcttcacga
     1081 cgaccttgcc tttgatctga cgcgcttcca tggcgcgcaa cgcgtccgcc gtttcggcga
     1141 gagagaaaac gcgatcgata tgcggccgga tcttgccctc tacgcaccac tggatgatcc
     1201 gggcgatctg atcgcgaaag ccggggaggt tggacgcaac gtgccgtccc cagaaaacgc
     1261 ctgcaatttc gcaacctttc aacagcacga ggttcagtgg gattttcgga atgtcgcccg
     1321 ccgcgaatcc cacaacgagg tagcgtccgc cccaggcgat gccacgcagg gctggctctg
     1381 catacgggcc gccgatgcag tcgtaaatca agtccgggcc tttgccgccg ttcagggcgc
     1441 gaaccgcctc tttcacattg atgccggtta gattgagaag ctgcgccgcg ccccgttccg
     1501 cacatatctt gagcttctcg tccgaggtgc ccgcggccac gacctcggcg ttcaggcaag
     1561 cagccacctc gacagcagcg agtccggcgc cgccgctcgc gccaaggaca agcactgtcc
     1621 gccccggcca aagatggcca cgatcctcca gcccgtgaag agcggtgccg tacgcgatgg
     1681 ggatggacgc agccgcttca tcggagatgc cgtcgggcaa agcaacgacg cggcccgcgg
     1741 gcgcgatcgc ctcttcccgg cacccattat agccgatata ggcgagcacc cgctggccaa
     1801 cggaaagccc ctccacctcc ggcccgagcc gggcgaccgt ccccgcaagc tcaccacccg
     1861 gcgagaatgg caactccggt ttcgtctgat acctgccgcg aacgatcagg gtatcgaaga
     1921 aattcagtgc gaccgccgaa acgcggatcg caacctcgcc cgcccccggc tcggaagcag
     1981 gaagatcgcc aacctcgagc gcgtcgggcc cgtcgagaga cttgcataga acagctttca
     2041 tcgcatacct cagtccgaac acctcatttt acgcgaaaag caagtgacat gcgtggatac
     2101 ttcgcaatcg gagccgaacg gatatcgaag cccatgaata tgggcaacct tctgcgttcg
     2161 gcccacgctt tcggcgcgaa gttcttcttt acgatcggcg cgcatccgcg tgctttcgat
     2221 gcaaagtccg acacgtccaa ggccgtccac catttgccgg tctatcattg ggcgaacgcg
     2281 gacgagatca tcctgccttt ccgctgcaag ctcgtcggcg tggaactgat cgagggcgcg
     2341 acagatttgc caagcttccg ccatccgctc catgcggcgt atgtgcttgg ccccgagctt
     2401 ggcgttctgt ccgaggggct cctctcgcgg tgcgatcatg tcatcaaaat tccgacggct
     2461 ttctgcatca acgtcgcaat ggcgggcgcg attgtcatgt atgaccgcgt gcggagcctt
     2521 ggctcgttcc cggatcgcat ggaagcgcca gccccacatc ttcgggcgcg gatcgacaag
     2581 cttccgacag accggtgacc gaggcattga tttgccttga cggacaggcc gagatccaga
     2641 aagggtgaaa agcattgaaa aacgagaatt tggcatgcgc ggcggcctga ttattccggc
     2701 atcatttctt atcctgctgg ctccttgtgc gctcgcgcaa cagtcggata tcgagcaagt
     2761 cactcctcag ccttcacagc agcccaaggc agcgcccgcc aagccgaaac ccaagcccgc
     2821 tccggcaaaa aagcctgttc cggtcgccgc tcccgaggtt aaagcacgcc ttctcgaacg
     2881 aataaaggat tggacggtct acatttatga gggcgccgag ggacggatct gtttcgcggc
     2941 ctcggcaccg accgatatgc agcccaaaac cgcgaagcga acgcccgtca tcttctacgt
     3001 gacgacttgg cagaaggacg gcgttcatac cgaggtcagc gtgcggcagg gctacgccat
     3061 gaaggcgaac gccgtggcca cggtgacggt cggcggccag aatttctcgc tctcg
//
LOCUS       DATMGX010000619         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_170814, whole genome shotgun sequence.
ACCESSION   DATMGX010000619 DATMGX010000000
VERSION     DATMGX010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170814"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /gene="mscL"
                     /locus_tag="VNR65_10095"
     CDS             complement(<1..236)
                     /gene="mscL"
                     /locus_tag="VNR65_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWJ19064.1"
                     /translation="MLKEFREFIIKGNAFDLAVGVIIGAAFGGIVTSLVGDILMPPIG
                     ALMGDLNFSDYFVDLTSLKAEALGSEAVQVPASY"
     gene            complement(360..896)
                     /locus_tag="VNR65_10100"
     CDS             complement(360..896)
                     /locus_tag="VNR65_10100"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ19065.1"
                     /translation="MKTTLTAVAALMIAVSPLAAQTPNFIPEQNQSEVLGTDFVGTQV
                     VSKDNQPLGKIANLVFDQSGHIELAVIGVGGFLGIGEKEVAVPFDALKSDVVNNKHVF
                     TVDVTKDQLKAAPAFKTLNDQARRDLIAKWRTKAEQSWADLKSKTGKAYEDTKERVNE
                     AREKVEHKLEKAEEPKAQ"
     gene            complement(1123..1611)
                     /locus_tag="VNR65_10105"
     CDS             complement(1123..1611)
                     /locus_tag="VNR65_10105"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWJ19066.1"
                     /translation="MSGQDHKGQEPVNPRIVPLPPVVLLLFLAAGYGLQRLFPIGGGE
                     PAIESVIAGNALMAIAFVIAFLGAWEMFKTRTTIRPGGSATALVTSGIYRRTRNPMYL
                     SFLFFIIGLGLAFANPWMILFAPLLLLYVQERIIKREEGYLTARFGPEYIDYRKKVRR
                     WF"
     gene            1637..>2336
                     /locus_tag="VNR65_10110"
     CDS             1637..>2336
                     /locus_tag="VNR65_10110"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HWJ19067.1"
                     /translation="MDNAEAIAFVTPLERELEPRLLAAIDAKAKPQGSLGRIETLAVH
                     IGLVTKSPKPNLGKAALLVFAGDHGIVAEGVTAYPSEVSTLIAQMVLDGKAGANIAAK
                     GAGAEVFLIDSGLKTPPQPHKALIGKSVRRSTRDSLLEPAMTDSECRDALRAGYDVAT
                     RLKTERFGILALGEIGIGNTSAASLVAHCVTGLPLEKLVGPGAGAPPGGLAHKRDVLS
                     RAHARAPVRDGMEAM"
BASE COUNT          531 a          684 c          638 g          483 t
ORIGIN      
        1 tcatacgaag cgggtacctg aactgcctca ctgccaaggg cttctgcctt cagagacgtg
       61 aggtcaacaa agtagtcgga gaaattcaaa tcgcccatca gcgcgccgat tggcggcata
      121 aggatatcac caacaagcga cgtgacgatc ccgccaaagg cggcgccgat gatcacaccg
      181 accgcaagat cgaatgcatt tcccttaatg atgaactctc ggaattcctt caacatctca
      241 aataacccct cccagattga tctcaagccg agactcacta ttgggagaaa tgggcaggga
      301 caggtcaagg caatgaaaaa ggaggccgaa gccccctttt ttgcgtccgg atcacagagt
      361 tactgagcct ttggctcctc tgccttctcc aacttgtgct cgaccttctc gcgcgcctcg
      421 ttgacgcgtt ccttggtgtc ttcgtaagcc ttgccagttt tagacttgag atcggcccag
      481 ctctgttcgg ccttggtgcg ccactttgcg atcagatcac ggcgtgcctg gtcattcagg
      541 gttttgaagg ccggggccgc tttgagctga tcttttgtta cgtcgacagt gaagacgtgc
      601 ttgttgttga ccacatccga cttcaacgcg tcgaacggca cagccacttc cttttcaccg
      661 ataccaagaa agccgccaac gccgatcacg gcgagctcga tgtgtccgct ttggtcgaac
      721 acaagattcg cgatctttcc gagtggctgg ttgtccttgg aaacaacctg ggttccgacg
      781 aaatccgttc caagaacctc agattgattc tgctcgggga tgaagttcgg cgtttgtgcc
      841 gcaagagggc tcaccgcaat catcaaggcg gctacagccg taagcgtcgt tttcatgcaa
      901 aaactcccat gttcgccgtg catcaaatga cacgaggtaa gacacgatgg tgtagaaaaa
      961 gttccggtac ggatgaatgg caatgctgtg cgcaaattag ggcaaagcct gccacctaac
     1021 cgtcaaaaac cctggctcgg gtttgggtaa ccattcttac actgacgata tggaggcggg
     1081 caagcaggat ggcaacggcg ccccaaaaag gaagtggatg ccttaaaacc aacgccgtac
     1141 cttcttgcgg taatcgatat actccgggcc gaagcgggcg gtaaggtagc cctcttcgcg
     1201 cttgatgatg cgttcctgga catacaagag cagaagtggc gcgaatagaa tcatccaggg
     1261 attcgcgaag gccagtccaa ggccaataat aaaaaagaga aagctcagat acatcggatt
     1321 gcgtgtgcgc cgatagatgc cggacgtaac cagcgcagtc gccgagccgc ccggacgtat
     1381 cgtggttcgc gtcttgaaca tctcccaagc gccgagaaac gcgatcacga aagcgatagc
     1441 catgagtgcg ttgcctgcaa tcacgctctc gattgccggc tcaccgccgc cgatgggaaa
     1501 aagtctctgc agcccatacc ctgccgccag aaaaagcaga agcacgacag gggggagcgg
     1561 gacaatccgt gggttgactg gctcctgtcc cttgtgatct tgtccgctca tcgtctcccc
     1621 tccttccggt gtctccatgg ataatgctga agcaatcgcg tttgtcacgc ctcttgaacg
     1681 cgagttggag ccgcgcctcc tcgccgccat cgatgctaaa gcgaagcccc aaggctcgct
     1741 cggacgaatc gaaacgctcg cagtccatat cggacttgtc acgaaatcgc caaagccaaa
     1801 tcttgggaaa gcggctcttc tcgtatttgc gggcgaccac ggcatcgtgg cagaaggcgt
     1861 gacggcctac ccttccgaag tttcgacttt gatcgcccag atggttctcg atggcaaagc
     1921 cggggcaaac atcgcggcaa agggagctgg cgccgaggtt ttcctgatcg attcgggcct
     1981 gaagacgcct ccccagcccc acaaggcgct gatcggaaag agcgtgcgcc gctcgacgcg
     2041 tgattccctg cttgaaccgg caatgacgga cagcgagtgc cgcgacgcac ttcgtgccgg
     2101 ttatgatgtg gcaacccgtc tcaaaaccga acgcttcggt atccttgcat tgggtgagat
     2161 cggcatcgga aatacaagcg cagcctccct tgtcgcgcat tgcgtaaccg gcctgccgct
     2221 cgaaaagctt gtcggtcccg gtgccggagc accccccggc ggcctcgctc acaagcgcga
     2281 cgtgctttcc cgcgctcatg cccgcgctcc tgtccgcgac ggcatggaag ccatgc
//
LOCUS       DATMGX010000620         2416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_180494, whole genome shotgun sequence.
ACCESSION   DATMGX010000620 DATMGX010000000
VERSION     DATMGX010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180494"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /gene="pstS"
                     /locus_tag="VNR65_10115"
     CDS             <1..801
                     /gene="pstS"
                     /locus_tag="VNR65_10115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWJ19068.1"
                     /translation="DFGASDMPLRPDELAKAGLGQFPLVIGGVVPVVNIDGIGPGQLR
                     FTGGALADIFMGKIKNWDDPALRALNPDVALPAAPITVVHRTDGSGTTFNFANYLSKV
                     SSEWKEKVGEGVNVEWPVGVGGKGNEGVAAYVGLAKNSISYVEYAYAIRNNLAFGLVQ
                     NRAGRFVQPGAQTFQTAAAAAAWENTKDFYRVMTDAPGDEAYPITATSFILMPKQPKD
                     RGRSKIAIDFFRWSLENGQADADALNYVPLPADLIEKIEKYWKSDFGS"
     gene            complement(902..2188)
                     /gene="glp"
                     /locus_tag="VNR65_10120"
     CDS             complement(902..2188)
                     /gene="glp"
                     /locus_tag="VNR65_10120"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWJ19069.1"
                     /translation="MNARPELSTVRAMDDCLVNLRLLDLADAREIILHDVEEIRGNET
                     SPLGSLVGRVAAADVTSAVCLPRFDNSAVDGFGLHAADLQKHAPITLSIVGRVAAGHD
                     TSTKLEPGKTFRILTGARIPDGVAAVILEERTTRSTADVTLHFVPEMGANIRGRGEDV
                     SSGTIIVPRGTLLDARHLAILAASGSARISVKRRLRVGVLSTGDELVEPAVKTAPSAI
                     IDTNRPMLLALLGSQAIDVVDLGITADDRGGIARTLREAASHLDLLITSGGVAGSDAD
                     HLEPAILAAGGRCRTLKLALRPGKPIGKGVVDNMQVLALPGNPVAALVNFLLFGRPLV
                     RTLLGAMDGAPLAPIARAAGMFGHRAGRTEFVPVRIERFGADGLPIVSKLGRGGSARL
                     LPLVMSDGFAEIGPEVGDLDDGAIVRFHPFSTGFAL"
BASE COUNT          497 a          720 c          758 g          441 t
ORIGIN      
        1 gatttcggcg cctcggatat gcctcttcga ccggacgaat tggccaaggc cggtctgggt
       61 cagtttccgc tcgtcatcgg cggcgtggtt ccggtcgtga acatcgacgg aatcggtcct
      121 ggtcagctcc ggttcaccgg aggcgcgctc gcggacattt tcatgggaaa gatcaagaat
      181 tgggacgacc ctgctctaag ggctctgaat cccgacgtgg cgctgcccgc agcgccgatc
      241 acggtcgtgc atcgcaccga cggctccggc accaccttca acttcgcgaa ctacctttcc
      301 aaggtcagtt ccgaatggaa ggagaaagtg ggagagggcg tcaacgtcga gtggccggtg
      361 ggggtgggag gcaagggtaa tgagggcgtc gccgcatatg tcggcctggc caagaactcc
      421 atcagctacg tggaatacgc ctatgccatc cggaacaatc tggcgtttgg cctggtccag
      481 aaccgggctg ggcgcttcgt gcagcctgga gcccagacct ttcagacggc cgctgccgct
      541 gccgcttggg aaaacaccaa ggacttctac cgcgtcatga ccgacgcgcc gggcgacgaa
      601 gcatatccga tcacggcgac ttcattcatc ttgatgccga agcaaccgaa ggaccgcggc
      661 agatcaaaga tcgcaatcga tttcttcagg tggtccctgg aaaacggtca agccgatgcg
      721 gacgccctca actacgtgcc gttgcccgcg gacctgattg agaaaatcga aaaatactgg
      781 aagagcgatt tcggatcatg atgccgaaat ggatgacgtc atctaccaca atccggactg
      841 cggaacgtcg cgcgaccaag ccccgacgac gtccacgaca gacccttgaa gcttgtgagg
      901 gctaaagagc gaaccctgtc gagaacggat gaaacctgac gatcgcgccg tcgtcgaggt
      961 cgcccacctc gggaccgatt tcggcgaagc cgtcggacat gaccagaggc agcaggcggg
     1021 ccgagcctcc tcggccaagc ttgctgacaa tcggaaggcc atctgcgcca aatcgttcga
     1081 tgcgaaccgg gacgaactct gttctgcccg cccggtgacc gaacatcccg gcggcgcggg
     1141 caattggggc gaggggggcg ccgtccatgg caccgaggag cgttcggaca agtggccgac
     1201 cgaacaggag aaagttcact agcgcggcta ccggatttcc cggaagcgcc aagacctgca
     1261 tgttgtcgac gacgcctttg ccaatgggct tcccggggcg aagtgcaagc ttcagcgttc
     1321 tgcagcggcc cccggcggct agaatcgccg gctcgaggtg gtcagcgtcg ctgccggcga
     1381 cgcctccgga ggtaatcagg agatcgagat gcgatgctgc ttcccgcagc gtgcgagcga
     1441 tgccgccgcg gtcatccgca gtgataccga ggtccacgac gtcgatggcc tgggagccga
     1501 gaagtgcaag caacatcggc cgattggtgt cgatgatggc gctcggcgcg gtcttgaccg
     1561 cgggctcgac gagttcgtcg ccggtcgaca gaacgccgac tcgaagccgt cgcttcacag
     1621 aaatgcgcgc acttccagat gcagccaaaa tggccaaatg tctggcatcc agtaacgtgc
     1681 ctcgtggaac gattatcgtt ccggatgaga cgtcctcgcc gcgccccctg atgttagcac
     1741 ccatttccgg cacgaagtgc agggttacat cggctgtcga tctggtcgtg cgctcctcca
     1801 aaatgacggc ggcgaccccg tcaggaattc gcgctcccgt caggatccgg aaggtcttcc
     1861 ccggctccaa tttcgttgat gtgtcgtggc ctgcagcgac cctgcccacg atgctcaacg
     1921 ttataggcgc gtgcttttgc aggtcggcgg cgtgaaggcc gaatccgtcc acggccgagt
     1981 tgtcgaagcg gggcaggcag acggcggagg tgacgtcagc ggcggccacc cggccaacca
     2041 acgaaccaag gggggaggtt tcattgccac gaatttcctc cacgtcatgc aggatgattt
     2101 cgcgggcgtc ggcgagatcg agaagccgca ggttaacaag gcaatcgtcc atcgcccgca
     2161 cggtggaaag ctctggtcga gcattcattt gaaaccctca atccggagca agagcaggag
     2221 agggcgccga tgtccggcgc cctctaagtt tttaagcagt atattccttc gacttgaacg
     2281 tcagatgccg gacgccttcc ggggcgtcag agatcttgcg gatgcttccc caagtctgct
     2341 tatagttcgg gatgatcagc tcgttcacgc ccttggaaac aacgttgcct tgaacgccgg
     2401 tgggggatgc gaacag
//
LOCUS       DATMGX010000621         2000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_428204, whole genome shotgun sequence.
ACCESSION   DATMGX010000621 DATMGX010000000
VERSION     DATMGX010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428204"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..919)
                     /locus_tag="VNR65_10125"
     CDS             complement(140..919)
                     /locus_tag="VNR65_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ19070.1"
                     /translation="MQLEGKRALITGAGSGIGQRLAIEAAERGVKLIITGRRKEALDN
                     TLSKLPAKAHYAFASDVTKPEDRTALMEAVRRHWGGLDILINNAGVVPVGPLAQSTDE
                     ELEAAVATNLVAPISLVRQALPLLKRGESSRVVNIGSVLGDIPYPLMAIYSATKSGLR
                     GFSIALRRELEPLGIGVTYAAPRTTRTAAASALGPLVQAFGLKFDKPEAVAKLIWDAV
                     ERDAKSVYPAGPEKMFVLVQRLFPHLVDRSVAEQLAKVGAA"
     gene            complement(909..1580)
                     /locus_tag="VNR65_10130"
     CDS             complement(909..1580)
                     /locus_tag="VNR65_10130"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWJ19071.1"
                     /translation="MKPYDRLYAETAASRNEFLSIPLVKQALVAGGSRALYLDFLTQA
                     YHHVKHTFPLLSLAAARTKDERYQDALFQYMKEERGHDKWILDDIQAFGGDAKRVAND
                     KPGLACEIMVGYAYYCIERVSPYAILGMVHVLEGLSTMLAHKVAGAVKNSLKSDGSAG
                     FSYLMSHGSLDVEHVALFKNLLNGIEDKETVDIMVEASKVFYRLYGAIFIDLGAAHLE
                     VAHAA"
     gene            complement(1577..>2000)
                     /locus_tag="VNR65_10135"
     CDS             complement(1577..>2000)
                     /locus_tag="VNR65_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19072.1"
                     /translation="ADGYLTVHGRRDNLIITPFGRNLSPEWIETILLGDPRLRACIIA
                     QLGEPAQLTALLIPSPAGEAWLKAAAQPDIVKHLAHALHAAPDYARPKAAIVATFAEV
                     AASGLFTPNGRIRRKAALKYLEERIESHPAENAKKAVA"
BASE COUNT          371 a          565 c          642 g          422 t
ORIGIN      
        1 tgtcgagtgc tggatcactc gcggcgttgg acatggaaga gccggcggcg agtaggatta
       61 ggccaatgcc tcccgcgata atggcgcgta gattcactct cattattcaa tccctttctt
      121 caatgatgtg attttgattt caggcggcgc cgactttggc gagttgctcg gcaacggacc
      181 tgtcgacgag gtgcgggaac aggcgctgga ccagcacgaa catcttttcc gggccagccg
      241 gatagacgct cttggcgtcg cgctctaccg cgtcccagat gagttttgcg acggcttcag
      301 gcttatcgaa cttcagcccg aaagcttgca caagtgggcc aagcgcgctc gctgccgccg
      361 tcctggttgt gcgcggcgcg gcgtaggtga cgccaatgcc gagcggctca agctcgcggc
      421 ggagcgcgat ggagaagccg cgcaagcccg atttcgtggc cgagtaaata gccatcagcg
      481 ggtaagggat gtcgccgagg accgacccga tgtttaccac gcgggaggac tctcctcttt
      541 tgagcagagg cagagcttgg cgaacgagag atataggcgc cacaagattg gtcgcgaccg
      601 cggcttccag ctcttcatcc gtggattgcg cgagcggtcc gaccgggacg acgccggcgt
      661 tgttgatgag aatatcgagg ccgccccaat gccggcggac cgcctccatc agtgcggtgc
      721 gatcctcggg cttcgtcacg tcgctcgcga acgcataatg cgccttggcg ggaagcttcg
      781 acaatgtgtt gtccagcgct tccttgcgcc ggccggtgat gatcagcttc acgccacgct
      841 ccgccgcctc gatggcgagc ctttgtccga tccccgatcc tgcgcctgtg atgagcgcgc
      901 gttttccctc aagctgcatg agcgacctcc aagtgagcgg cgcccaagtc gataaagatt
      961 gcgccataaa gccgatagaa aaccttcgag gcctcgacca tgatgtcgac ggtctctttg
     1021 tcttcgatgc cgttcagaag gttcttgaaa agagccacgt gctcgacgtc gagcgagccg
     1081 tgagacatga ggtaggagaa gccggcgctg ccatcggact tcaggctgtt cttgacggcg
     1141 ccggcaacct tatgcgccag catggttgag aggccttcca gcacatggac catgccaagg
     1201 atggcgtaag ggctgacgcg ctcgatgcag taataggcgt agcccaccat gatttcgcag
     1261 gcgagccctg gcttgtcgtt cgcgaccctt tttgcgtcgc cgccgaaggc ttgaatgtcg
     1321 tccaggatcc atttgtcgtg gccgcgctcc tctttcatgt attggaacag cgcgtcttga
     1381 taacgctcgt ctttcgtgcg cgccgcggcg agcgagagga gcggaaaggt atgcttcacg
     1441 tgatggtagg cttgcgtgag aaaatcgaga tagagcgcgc gcgagccgcc agccaccagc
     1501 gcctgtttca ccagcggaat ggacaggaac tcatttcgtg aggcggctgt ctcagcgtaa
     1561 agacggtcgt atggtttcat gcaactgctt tctttgcgtt ttctgcgggg tgtgactcga
     1621 tgcgctcttc gagatatttc aaggccgcct tacgccggat tcggccattg ggcgtgaaca
     1681 ggccgcttgc ggcgacttct gcgaaggtcg ctacgattgc ggctttgggg cgcgcataat
     1741 cgggcgcggc gtggagcgcg tgtgcgagat gcttcacgat gtcgggttga gcagccgctt
     1801 tgagccacgc ttcgcccgca ggcgacggga tgagaagggc tgtcagctgc gcgggttcac
     1861 ccaactgggc gatgatgcac gccctgagcc ttggatctcc gagaagaatc gtctcgatcc
     1921 attcagggct caagttgcgg ccgaagggcg tgataatgag attgtcacgg cggccatgaa
     1981 cggtgaggta gccgtcggcg
//
LOCUS       DATMGX010000622         2483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_438521, whole genome shotgun sequence.
ACCESSION   DATMGX010000622 DATMGX010000000
VERSION     DATMGX010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438521"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1352)
                     /locus_tag="VNR65_10140"
     CDS             complement(90..1352)
                     /locus_tag="VNR65_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ19073.1"
                     /translation="MPQSARPPSTSWVARPKSTLILREILSIAVDSFRADKVRATMTA
                     LGMVIGTFSLVLVVTIALTGKQYVLNEIQNIGANLIWAEYAGSANAGASAGTADYLTV
                     NDMLAVEQQVPGVQEASPVLNLHEPILVGNGKQRDMLILGVDPEYVTVRRIAVSSGRF
                     FDRQDSQSHHKVALVTEDFAARQYGGIEFAAGHEIDIQGLPFVVIGAFREGVETFGQS
                     EIQDDTVLIPYSVARYLTDTNAVNQIYFSMSDAGSVPRASQRILAVLKARHRAESVYT
                     VGDLTQVLGLVAKTANAFTIILLLFATITLVAGGVGIMNIMLATVSARIREIGIRKAV
                     GATRREIQMQFLSEAVMISLIGGTIGTLAGLALPFSLRIFTQYRLPVSALSALIAMLV
                     SCLIGITFGTLPAKNAARLDPVESLRYE"
     gene            complement(1359..1733)
                     /locus_tag="VNR65_10145"
     CDS             complement(1359..1733)
                     /locus_tag="VNR65_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWJ19074.1"
                     /translation="MSEFIFFFFIVVFGTTGEIFMSRAMKETGEVKDFRPVALVEAIF
                     RALRVRWMWFGVGMMAIGFFSLLAVLSFENVSFVVPVTALSYVVGALGGRFFLGERVS
                     LQRWTGILLVCAGVTLVLIGRS"
     gene            complement(1736..2113)
                     /locus_tag="VNR65_10150"
     CDS             complement(1736..2113)
                     /locus_tag="VNR65_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19075.1"
                     /translation="MILIMVLAGPLGNVLLGKAMKQVGPVHPWPPAELLHVFIRVFTS
                     GTIWLGIGSQLVFFAAYMIVLSWADYSYVQPASSIAYAVVALLASTWLGEVVPPLRWV
                     GIAVICLGVFLVGHTHPSTTESG"
BASE COUNT          609 a          729 c          675 g          470 t
ORIGIN      
        1 gaaagggtag gactggcaac tgactactga ctactggcta ctgctttacg aatcaccttc
       61 acgccactca aaatatctta cgtctcatct cattcgtatc ttaacgactc cacgggatct
      121 aatctcgcag cattcttggc cggcagggta ccgaaggtaa tgccgatcaa acaggagact
      181 agcattgcga tcagcgcgga aagcgccgat accgggagcc ggtactgggt gaagatccgg
      241 agggaaaacg ggagcgccag cccggccaag gtgccgatag ttccgccgat cagggagatc
      301 attaccgcct cagaaaggaa ttgcatctgg atttcgcggc gcgtcgctcc aaccgctttg
      361 cggatgccga tttctcgaat acgggcgctc acggtggcca gcatgatgtt cataatacct
      421 actccaccgg caaccagagt gatggttgca aacagcaaga gaatgatggt aaatgcattt
      481 gccgttttcg ccaccaggcc aagcacctgg gtgagatcgc cgacggtata aaccgattcg
      541 gctcgatgcc gcgcctttag caccgcgaga attcgttgcg aggcacgggg aacgctgccg
      601 gcatccgaca tcgaaaaata aatctgattg acggcgttcg tatctgtcaa ataacgcgcc
      661 acggaatatg gaataaggac cgtgtcgtct tgtatctccg actggccaaa cgtctccacg
      721 ccctcgcgaa aggcgccgat cacaacgaac ggcaatcctt ggatgtcgat ttcgtgtccc
      781 gccgcgaact cgattccgcc gtattgccgc gcggcaaagt cttccgtgac cagagcgacc
      841 ttgtggtggg attgggagtc ttgtcgatcg aaaaacctgc ctgaactcac ggcgatgcgg
      901 cgcacggtca catattccgg gtccacaccc aggatcaaca tgtcacgttg cttgccgttg
      961 ccaaccagta tcggttcatg caaattgagc accggcgacg cttcttgcac gcctggcacc
     1021 tgctgttcca cggctaacat gtcgttcacg gttagataat ccgccgtgcc ggcgctcgcg
     1081 cccgcatttg ccgacccggc gtattcggcc cagatgagat tggcaccaat gttctggatt
     1141 tcattcagaa cgtactgttt tcccgtcagc gcgatggtga cgaccagcac cagcgagaaa
     1201 gtgccgatca ccattcccag ggcggtcatg gtggcgcgca ctttgtcggc gcgaaacgaa
     1261 tccacggcga tcgagagaat ctcccgcaag atcaacgtgc tcttggggcg cgcgacccat
     1321 gaggttgaag gtggtctcgc cgattgcggc ataaaccgct aactcctgcc gatgagcact
     1381 aacgttacgc ccgcgcaaac cagaagaata ccggtccaac gttgcaagct gacacgttct
     1441 ccgaggaaaa acctgcctcc caacgcgccc acgacgtagc tcagggccgt caccggtacc
     1501 acaaagctta cattttcaaa ggaaagcacg gcaagcagcg agaaaaaccc gatggccatc
     1561 atcccgaccc cgaaccacat ccaccgaacg cgtaaagcgc ggaagatcgc ctcgacgagc
     1621 gctaccggcc tgaagtcttt cacttcgccc gtctccttca tggcgcgcga catgaatatc
     1681 tcgcccgtcg ttccgaagac aacgatgaag aaaaagaaga tgaactcaga caccactagc
     1741 cactctccgt ggtactggga tgggtgtggc cgacgaggaa caccccgaga caaatgaccg
     1801 cgatgccaac ccatcgtaat ggagggacga cttcgcccaa ccacgtcgag gccaatagcg
     1861 caactacagc gtaagcgata gaagaggctg gctgcacgta gctatagtca gcccacgaca
     1921 gaacgatcat ataggctgcg aaaaacacca gctgggaacc gattccgagc catatcgtgc
     1981 cagaggtgaa cacgcgaata aagacgtgca gcagttccgc gggcggccag gggtgcaccg
     2041 gccccacttg cttcatggcc ttgcccagca gaacgttgcc taaaggaccg gcaagcacca
     2101 tgattaagat caagataaag gttttgagat gcagtcgcct gggagattcc attggccgtg
     2161 gtcagtcgct ccaaatgcag agaggcatag ctgtatggta tatcaaggaa cacccggccg
     2221 ggaggcagca agcgcgtacc catatcggag cggccaaccg aggtgtacaa ccaccccgcc
     2281 gcggaatcgt gcggcgctac aggacgcgga aatcgagcca cccggctgtc atcctccaaa
     2341 tcctaagatg ctatcctaga gtagctgttc tttgcaggat tcccatcgcc atgggagatg
     2401 aaaattgctg ggccgcattg tcgggtcgcc ctcaccttgc tcaaggcggc aagtcgcggt
     2461 aggctgatca attcggcatc gag
//
LOCUS       DATMGX010000623         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_387159, whole genome shotgun sequence.
ACCESSION   DATMGX010000623 DATMGX010000000
VERSION     DATMGX010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387159"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /gene="ppk2"
                     /locus_tag="VNR65_10155"
     CDS             <1..652
                     /gene="ppk2"
                     /locus_tag="VNR65_10155"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HWJ19076.1"
                     /translation="AGKGGVIKRITQSLNPRICRVVALGTPTERERGQWYFQRYIAHL
                     PAPGEIVLFDRSWYNRAGVEHVMGFCSLDEYHEFLRSCPLFEEMLVRSGIYLLKYWFS
                     VNDEEQERRFQERIRNPAKRWKLSTMDLESRRHWVEYSKAKDVMLAHTDHEKTPWWVV
                     NADNKKRARLNCISHLLKQIPYHDMTPVEIEVPPRQDDIGYKRPKKSTQRFVPDVY"
     gene            complement(926..1957)
                     /locus_tag="VNR65_10160"
     CDS             complement(926..1957)
                     /locus_tag="VNR65_10160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWJ19077.1"
                     /translation="MPSGSVDLVFADPPYNLQLGGDLLRPNNTRVDGVNHAWDRFDNF
                     ASYDQFTRAWLSECQRVLKPNGAIWVIGTYHNIFRLGTALQDLGFWLLNDIIWLKTNP
                     MPNFKGTRFTNAHETLIWAARDEAARPTFNYDSLKAFNDDLQMRSDWLLPICSGPERL
                     RDDGGRKAHPTQKPEALLARVMLATTKPGDLVLDPFFGTGTTGAVAKTLSRRYLGIER
                     DPDYVAAAKARIAAVTPCDAKTAFPIVSKRSEPRVPFGMVLQLGMLRPGDVLFDAKAK
                     IRAQVMADASVVWDGVRGSIHSVGAKAQGRGACNGWTFWHFEKPKAGLAPIDLLRAEV
                     RRTIMPAAA"
     gene            3173..>3441
                     /locus_tag="VNR65_10165"
     CDS             3173..>3441
                     /locus_tag="VNR65_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19078.1"
                     /translation="MLQPIFHPAMMIMSRLRFALKLGLIGALFLAPLAGMTYFLDGQI
                     TKDIKFALVERLGVRQLIPARQLLQIMQTHRRISQLMAAGDQEAR"
BASE COUNT          746 a         1003 c          992 g          700 t
ORIGIN      
        1 ggccggcaag gggggcgtca tcaagcgcat cacccagagc ttgaacccgc gcatctgccg
       61 cgtcgtggcg ctcggcacgc cgacggagcg cgagcgcggt caatggtatt ttcagcgcta
      121 catcgcccac ctgccggcgc caggagaaat cgtcctcttc gaccgcagtt ggtacaaccg
      181 cgccggcgtc gaacatgtca tgggcttctg ctcgctggac gagtaccacg agtttctgcg
      241 ctcttgcccg ctctttgagg aaatgctggt tcgctccggc atctacctct tgaagtactg
      301 gttttcggtc aacgacgaag agcaggaacg gcgtttccag gagcgcatac gcaatcccgc
      361 caagcgctgg aagctcagca cgatggacct cgagtcgcgc aggcactggg tcgagtactc
      421 caaagcgaaa gatgtgatgt tggcccacac cgaccacgag aagacgccat ggtgggtcgt
      481 gaatgccgat aacaaaaaga gggcaaggct gaattgcatc tcgcatttgc tgaagcagat
      541 tccctatcac gacatgacgc cggtcgaaat cgaagtgcct ccgcggcagg atgacatcgg
      601 atataagcgg cccaagaagt cgacccagcg ctttgtgccc gatgtttatt gacctggcat
      661 tggactcgta gagagaattg gaagcgatag gtcatcgggg ccaacgcgaa caaattccgc
      721 tgctgaatgt caagctgcca agatgcgttt tcacgcggcc aggacccaac acagcccaag
      781 acggaaccta tctatccacg gccgggctga cggagggcgc cgcaatgccg tgcccatttt
      841 tggcaaagcc gagaatgttg gcaacagcga gtggcgcccg cgacgacaat ccgttggcgc
      901 gtccggtgga ggctgcttag gtgatttaag ccgccgcggg catgattgtg cgccggacct
      961 ccgcccttaa gaggtcaatc ggcgcgagcc ctgccttcgg cttctcgaag tgccagaacg
     1021 tccagccatt gcaggcgccg cgcccctgtg ccttcgcccc cacggagtgg atggacccgc
     1081 gcacgccgtc ccacacaaca gatgcgtcag ccataacctg agccctgatc ttggccttgg
     1141 catcgaacag cacgtcgccg gggcggagca taccaagctg aagcaccatg ccgaagggca
     1201 cgcgcggctc ggatcgtttc gaaacaatcg ggaaggcggt cttggcatcg caaggcgtca
     1261 cggcggcgat gcgagccttc gcggcggcga catagtctgg gtcgcgctcg atgcctagat
     1321 agcggcggct gagcgttttc gcgacagcgc ccgtcgtccc ggttccgaag aacgggtcga
     1381 gcacgagatc gcccggtttc gttgtcgcca gcatcacccg cgcgagcagc gcttcgggct
     1441 tttgcgtcgg gtgggccttg cggccgccgt cgtcgcgcaa ccgctcgggg ccggagcaga
     1501 tcggcagaag ccaatccgaa cgcatctgaa ggtcgtcgtt aaaggccttc agcgaatcgt
     1561 aattgaaggt cggccgcgca gcctcgtcgc gggcggccca gatcagcgtc tcatgggcgt
     1621 tggtgaagcg cgtgcctttg aagttcggca tcgggttcgt cttgagccag atgatgtcgt
     1681 tcaagagcca gaagccgagg tcctgaaggg ccgtgccaag ccggaagatg ttgtggtagg
     1741 tgccgatcac ccatattgcg ccgttcggct tcagcacgcg ctggcattcc gagagccacg
     1801 cgcgggtgaa ttgatcgtag gaggcgaaat tgtcgaagcg gtcccaggcg tggttcacgc
     1861 catcgacgcg cgtgttgttg ggccggagaa gatcgccgcc aagctgcaga ttataaggag
     1921 gatcggcgaa gaccagatcc acggagccgg atggcattcg gcgaagcacc tcgatgcact
     1981 cgccttgaac aatgctcccc tcggaatcgg ggctgaaact acgctccaac gactgaacgc
     2041 aacgcatgac tcaccatgac ataaaacgct tactgcgatc tctttatcag aagcttgagc
     2101 ataaagggtt aacgtaaacg cggagttcac aaccgtcgtg aaaatgcatc taaccagtca
     2161 cttctgtggg cgctgacagg gcttcccgcg gacaaggcct gcgcgtataa tcgggcgcct
     2221 ccccgatcgg ctctccatcg tcaaaaaatc gagtttggac caattcctgt ctgcttgaac
     2281 gtcaaggaag gcgtcatttt ggtccaaact cctccttact tggcctgcgc cgctatttta
     2341 caaaaatgcg ctttactggg acgttaactt atataagtca ttgatataca tggataaaat
     2401 agctcatggc gtgcgccatc caagccgcgg acggactgcg gcatggttaa ccggcgcggc
     2461 tggcatataa ttaacggact ttagcgtgtc ccatctctgg gaaaaataca atcagttaac
     2521 cctgtcgaag tggcccaatg aagggaaaac agccaacctt ttcaaggtga taggcgcatc
     2581 gccattttct gggccgaaga ggccgccaca attcgagcac atttcgttgg gccaaatgga
     2641 ccagaaataa ttccggcccg cttccgttaa ccaaaccccc gttaacactc cgcttggcga
     2701 cgcaccgctt gccagccctg cgccggacga tgggggaggc gtggccgcga acggatctga
     2761 actcccgcgg ggcgtactcg ctcaaaagta aaacccgcca acgatattct gccctttcgc
     2821 atacgcttca acagaacgga caagcgctag cgagagccac actttaagcg cgcttttcgc
     2881 ttggcctttt atctttcttt ttcggtccgg agcggcgcct tctcatgatc gcgttcgcga
     2941 atttccgcag gtgtcaggct tgcgcatcat gtcgtcccgt caaaacggga aatccagcac
     3001 gccgctgcta ttttgcaatt ggctcaacta tagtttcagc gttgctgcga tttgaaccag
     3061 ccttgcagct catcttcgct tcagcttaat tttatgggtc atcgcaacga gaacccaacc
     3121 tcgataaggc acacctttag atcgatgttt ctgcgatgga gcgcggtcaa caatgctgca
     3181 gccaatcttt catccggcga tgatgatcat gagccggctc cgttttgcgc tgaaactcgg
     3241 attaatcggc gcgctgtttc tcgcgcctct cgcagggatg acctatttcc tcgacgggca
     3301 gattacgaag gacatcaagt ttgcgctggt ggagcggctt ggcgttcgac agctcatccc
     3361 ggcgcgccaa cttctgcaaa tcatgcagac ccatcgcaga ataagtcagc tcatggcagc
     3421 gggtgaccaa gaggcgcgta a
//
LOCUS       DATMGX010000624         2726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_104836, whole genome shotgun sequence.
ACCESSION   DATMGX010000624 DATMGX010000000
VERSION     DATMGX010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104836"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(561..1232)
                     /locus_tag="VNR65_10170"
     CDS             complement(561..1232)
                     /locus_tag="VNR65_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19079.1"
                     /translation="MPYQFQRTAIFLPLTHSKSRCANKFKVKKHIDLDIKDGHFAFSR
                     KAEKIAKEAELDGIYILRTSLSDAACPSGDVVRSYKQLSRVERSFRTLKGVDLQIRPF
                     YHYLEKRVRAHILLAMLAYYVEWHLREAWAPLLFKDEHPPVADDPVSKALTSETAQRK
                     ASHQTTADGSIVHSFKTLLSELATRARTTTRIGQSEATFTRLVKATPLVETALKLVQQ
                     LPVAT"
     gene            1231..2457
                     /locus_tag="VNR65_10175"
     CDS             1231..2457
                     /locus_tag="VNR65_10175"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HWJ19080.1"
                     /translation="MRDTELLRGLLRVEDPWDVTESQLDLKLKRVDVRLEWKGPGRCP
                     KCDRECPKHDHRERVWRDLDLCSDQLFLHALVPRVDCPEHGVITVAVPWSTGRSEFTA
                     RFERLAIALLVEMSVKAVARRLDIGWDSVDGIMMRAVERGKQRRIKTVVRHLGIDEKA
                     FKKGHKYFTIVSDLDKGEVLWVGRGRKRESIDAFYHQLSQEQLDGIEGVAMDMWQPYF
                     ESTITHVPRAADKIVFDKFHITAYLTKAVDLTRRALMRDTSLDRTALKGTKYKWLHAY
                     RNLDRAERRELAVLRHEYKRLGRAWSIKENFAEFWRYRSESHARRFFAEWYRWATHSQ
                     LPEMVSVARTIKRHFENIITYIRKPITNAASEGLNSKVQMIKYRARGYRNEDRFEAAI
                     LFHCGGLDLYPTHSNS"
     gene            2466..>2726
                     /locus_tag="VNR65_10180"
     CDS             2466..>2726
                     /locus_tag="VNR65_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19081.1"
                     /translation="MGNWHNMPTKEKVSLEIQRFEGFAVRIRPAAGTTEKSSLKSYTR
                     NHERIARQSFSVADWKRRRFDTLYPSHEVDVLLANGKPAPPRT"
BASE COUNT          567 a          764 c          796 g          599 t
ORIGIN      
        1 tgctcagccg cacgacggcc gcgatctctt tgctctgcag cccgtcggct aatccctgaa
       61 tgatcgcgac ctctttgggt gaaacgatga tcctcatctg gttcctcagc gtgcggacgc
      121 cgctcgccgc caacgcgagc gccgccgaca tgaactccaa ttcgcggatg ccgcgtcact
      181 cgccggacca acgaggacgc tcgaactagg ggctcagtgt agacccgtcc gcggcatccc
      241 gaacatccgt actcgtactt atcttagcgt ttgtcgcagt ccgaacgccg gcggaaactg
      301 cgtaggcgcg cgacgggcgc cgcgcggtcg tcgcttgctc ttgcacggcg gacgagcaag
      361 gagcccgcct gtggtgctgc gtcggattgg gcttcggcgg ccggttaggc tcggcgtata
      421 ccatgagccg cttgcggctt cgcgccggcg tgtttgcgcc tatccggcgc aggatttcta
      481 acctgaactt acccctgggc tttccggtga aagccttata aatcaatggt tttcgccatt
      541 gttcgttccg ctcttggtgt ctacgtagcg acgggcagct gttgtacgag cttcaaggcg
      601 gtctcgacaa gcggtgtcgc cttgaccagc cgggtaaatg tggcctcgga ctggccgatg
      661 cgtgtcgtgg tgcgcgctcg cgtggcgagc tcgctgagca atgttttgaa cgagtgaaca
      721 atcgaaccat cagcggtggt ttgatgactc gccttgcgtt gcgcggtttc ggatgttaac
      781 gccttactca ccggatcgtc ggccaccggc ggatgctcat ctttgaataa gagcggggcc
      841 caggcttcgc gcagatgcca ttcgacgtag taggcgagca tagccaaaag gatgtgcgcg
      901 cggactcgct tctcaaggta gtgataaaac ggccgaattt ggagatcgac gcctttgagc
      961 gttcgaaacg aacgttcaac acgagagagt tgtttgtatg aacgaacgac gtcaccgctc
     1021 ggacacgcgg catccgagag gctcgtacgc aggatgtaga ttccgtcgag ttccgcctcc
     1081 ttcgctatct tttcggcttt gcggctgaac gcgaagtgtc cgtctttaat gtccaggtca
     1141 atatgcttct ttaccttaaa cttgttggcg catcgggact tcgagtgggt gaggggaagg
     1201 aaaatggcgg tcctttggaa ttggtagggc atgcgcgaca ccgaactgct gcgaggactg
     1261 ctccgagttg aggatccctg ggacgttacg gaatctcaac tcgacctcaa gctcaagcgc
     1321 gtcgatgtcc gactggagtg gaagggcccg ggccgttgcc cgaagtgcga tcgcgagtgc
     1381 cccaagcatg accaccgaga gcgtgtatgg cgtgatctgg atctctgtag cgaccaactc
     1441 tttttacatg cgctcgtgcc ccgcgttgac tgccccgagc acggcgtaat caccgtagcc
     1501 gtaccttggt caacgggccg ctctgagttc actgcccgat ttgagcgact cgccatcgcg
     1561 ttgttggtgg aaatgtccgt gaaggccgta gcccgccgct tggatattgg ctgggattcc
     1621 gttgatggga ttatgatgcg tgctgtcgag cgcggcaagc aacgtcgaat caagacagtc
     1681 gttcgtcact taggcattga cgaaaaggcg tttaagaaag ggcacaagta ttttacgatc
     1741 gtgtccgacc tcgacaaggg tgaggtctta tgggttggtc gaggccgtaa gcgtgaatcg
     1801 atcgacgcct tttatcatca gctttcgcaa gaacagcttg acggcatcga gggtgtggcg
     1861 atggacatgt ggcagcccta tttcgagtcg accatcacgc atgttccgag ggctgccgac
     1921 aagatcgtct tcgataaatt ccatatcacg gcttacctta ccaaagccgt tgacctgact
     1981 cgtcgggcgc tgatgcgtga cacgtcattg gacaggacgg cattgaaagg aacgaagtac
     2041 aagtggttac acgcctatcg gaatctggat cgcgcggaac gtcgcgagct cgccgttctg
     2101 cgtcacgaat ataagcggct tggcagagca tggtcgatca aagagaactt cgcggaattc
     2161 tggcgatatc gcagtgaatc acatgcgcgc cgcttcttcg ccgagtggta tcgttgggcg
     2221 acccactcgc aactgcccga gatggttagc gtagcccgga ccatcaaacg gcacttcgag
     2281 aacatcatta cttacattcg taagccgatc acgaatgccg cctctgaagg cctcaacagc
     2341 aaggttcaga tgatcaagta tcgtgctcgt ggctaccgca acgaggaccg atttgaggct
     2401 gcgatcctat tccattgcgg cggcctggac ctctacccga cccactcgaa ctcctgaagg
     2461 gccttttggg taattggcac aacatgccaa caaaagaaaa agtgtcccta gaaatccaac
     2521 gattcgaagg cttcgccgta cgaatccgac ccgctgctgg aacgacggag aaaagctccc
     2581 tcaagagcta tacgcgtaat catgaacgga tcgcgcggca atccttcagc gttgccgatt
     2641 ggaagcgccg gcgcttcgac accctctacc ccagccatga agtcgatgtt ctcctagcga
     2701 acggaaaacc agcacccccg cgtacg
//
LOCUS       DATMGX010000625         3468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_164866, whole genome shotgun sequence.
ACCESSION   DATMGX010000625 DATMGX010000000
VERSION     DATMGX010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164866"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VNR65_10185"
     CDS             <1..373
                     /locus_tag="VNR65_10185"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWJ19082.1"
                     /translation="LLLKKAWIRGTEMTKTQAAAAPKTAAKKPEKADIVTLRHLATEV
                     AETHGISQKQANEVLAEAITSIGKHLKKGARIRIAGLGTLEVRKRAARMGRNPATGEA
                     IKIKASKKVAFRAAKDLSEAI"
     gene            complement(418..714)
                     /locus_tag="VNR65_10190"
     CDS             complement(418..714)
                     /locus_tag="VNR65_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19083.1"
                     /translation="MTTVAPEPNPLSPHTEAGLNGRQGISLTRIYLPFVMVITIGVVL
                     TVAGYRVGGIVSDITRDKTETEKRLSAIEKEVTAIKNILAERTEYPQSCFPRSK"
     gene            complement(711..1055)
                     /locus_tag="VNR65_10195"
     CDS             complement(711..1055)
                     /locus_tag="VNR65_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19084.1"
                     /translation="MSVFLAFIASLGGRFIAGAAKLGLSTAGLALLGPLAPIITGIMQ
                     FVGAAVTAVGEILASLSKSAEGRVALALAVALLGFLYLRFHYIEEGKAIGRAEIAATH
                     KPCHVKTERRSR"
     gene            complement(1052..2113)
                     /locus_tag="VNR65_10200"
     CDS             complement(1052..2113)
                     /locus_tag="VNR65_10200"
                     /inference="COORDINATES: protein motif:HMM:NF017638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HWJ19085.1"
                     /translation="MASNNFERCLAITLKWEGGYSNHPDDPGGPTMRGIIQREYDAWR
                     KKNGKRLRPVHQIDEDELQSIYRNEYWDAMGCEELPAGFDLCVFDAAVNSGVGRARQW
                     IEAIPGNDIDAFCDLRLEFLQRLGRLWRVFGAGWHRRVVSIRVDARGMTGQLEAQPPD
                     DGCLHAGMRGAAVVALQEKLRALGYPAGTIDGIFGEQTYRAIVLFQHDHDLGGDPGIW
                     QPSYDSVLVKAAPMLPRRHNVTHRDLEKAGDKPIQRMNLLQRIFAWLFGGAAASETFG
                     SDNVLDSINGIRAVLEPLQNLWYWASTNRWLLLCAVFIAVIALIRVLRAEHVKAYRNF
                     DYQGPAQGPMYSSKTETVS"
     gene            complement(3064..>3468)
                     /locus_tag="VNR65_10205"
     CDS             complement(3064..>3468)
                     /locus_tag="VNR65_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19086.1"
                     /translation="SLDDELVAGHVRSLLASHLTDVVSSDQHTVKPWFLGKLDFAPPV
                     VDLSKKDFPLIGGRLDYIGGRVVAAIVYKRHGHVINLFVWPAGKQQAIPETADGYHLL
                     SWTRGGFSYAAISDLNPEELRDFKRALEESLA"
BASE COUNT          812 a          933 c          970 g          753 t
ORIGIN      
        1 attgctcctt aagaaggcat ggataagagg aactgagatg acgaaaacgc aagcggcggc
       61 ggcacccaag acggcagcaa aaaaaccaga aaaggccgac atcgtcaccc ttcgccatct
      121 agcaacggaa gtcgccgaaa cacacggcat ttctcaaaag caggcgaatg aagtgctcgc
      181 cgaagcgatc acgagcattg gcaaacatct gaaaaaaggc gcgcggattc gtattgccgg
      241 gcttggaact ctggaagttc gcaagcgggc tgcacgcatg ggccgcaatc ctgccactgg
      301 cgaagcgatc aagatcaaag ccagcaagaa ggtggctttc cgcgccgcca aagaccttag
      361 cgaagcgatt taaggaggcc ggtccggcct cctgtttctg acgctgcaat cccgtactta
      421 tttagatcgg ggaaagcagc tctgcggata ttcggtgcgc tcagcaagaa tgttcttgat
      481 tgcggtcact tctttctcga tggcgctcag tcgcttctct gtttcggttt tgtcgcgtgt
      541 aatgtccgac acaattccac cgaccctata gcccgccacg gtcagtacga caccaatggt
      601 gatgaccata acgaagggca ggtagatgcg agtcagggag atgccttggc ggccattcaa
      661 accggcttcg gtgtgcggcg acaaagggtt tggctctggc gcgactgttg tcatcgactg
      721 cgcctttctg tcttcacatg gcagggtttg tgtgtggctg caatttccgc ccttccgata
      781 gccttgcctt cctcgatgta gtggaagcgg agatacagga agccgagtag tgccaccgct
      841 aaggctagcg caacgcgacc ctcggcgctt tttgacaatg aggcgaggat ttcaccgacc
      901 gctgtgacag cagcgcctac aaactgcatg attccggtga tgataggggc caacggtccc
      961 agcagagcta agcctgccgt tgagagtccc agtttggcgg cgccggctat gaaacgtccc
     1021 cccagcgacg caataaatgc caagaatacg ctcatgatac ggtctccgtc tttgaggaat
     1081 acatcggtcc ttgcgccgga ccctgatagt cgaagttccg ataggccttg acgtgctcgg
     1141 cgcgcaaaac ccggatcagg gcgatgactg caatgaaaac ggcgcagagc aggagccacc
     1201 gattggttga ggcccaatac caaaggttct gcagcggctc caaaaccgcg cgaatgccat
     1261 tgatgctatc aagcacgtta tcgcttccga aagtttcgga tgcggctgct ccaccaaaga
     1321 gccaggcaaa aatacgttgg aggaggttca tcctctggat cggcttgtct ccggcctttt
     1381 caagatcgcg gtgcgttaca ttatggcgcc ggggcaacat tggcgcagcc ttgaccagaa
     1441 cactgtcata agaaggctgc caaatgccgg gatcgccacc aagatcgtgg tcatgctgga
     1501 agagaactat ggctcggtaa gtctgctcac caaaaatgcc gtcgatcgtt ccagcaggat
     1561 aaccgagcgc gcgaagcttt tcttgaagag cgacaacggc tgcacctctc atgcccgcgt
     1621 gcagacatcc atcgtctgga ggctgggcct ccaactgtcc tgtcataccg cgagcgtcta
     1681 cgcgtatcga aacgacacgc cggtgccagc cagcgccgaa tacgcgccat aaccttccga
     1741 gacgttggag gaattccaga cggagatcgc agaaggcatc gatgtcattg ccggggattg
     1801 cttcaatcca ttgcctcgcc cggccgacgc ccgaattgac cgcggcgtca aaaacgcaga
     1861 ggtcgaaacc tgctggcaac tcttcgcaac ccatcgcatc ccagtactca ttgcggtaga
     1921 tggactgcaa ctcgtcctcg tcgatttggt ggactgggcg caaccgcttg ccattcttct
     1981 tgcgccaggc atcgtactcc cgctggatga tccctcgcat agttggaccg ccgggatcgt
     2041 cgggatgatt ggaataaccg ccttcccatt tcagtgtaat ggcaaggcag cgctcaaaat
     2101 tgttcgaagc cattggctcc tctcttgtct gtttagatct ttgggtgtga ggaagtaatc
     2161 ggcggcaata agtgctgccg gaaagccggc agcgcataag cttctggccg caggctcatc
     2221 cttagcgtcg acaaaacata tgcagcacct gccttcccat cgcggttaca aaaatggatt
     2281 cgctcggaag ctggcgtact tccagtcatc gccgccagct gcggagatcg gcgtgttaat
     2341 aagttgcgta aagtttagag acgtcggcgg aagacagaac gcggtcgtag acaaccacct
     2401 gcttcagtgc tccattgaaa taacagtgct gcgcttcaca tttccggacg gaagttattg
     2461 gtggcgctta atggcgttta gctgtccaca tcgggtgaat tcgatgcaac gcaactacaa
     2521 atatacatag taatttttga ttagttataa gtaaaatgac gttagtgcat gtgtcaatag
     2581 attaattact gatgctgtcc gtgtaaacaa tttggtggca ttggaatggt tgctagcgca
     2641 caccatgagg cgcgcggcct cgtctgattt gaagtgctga ctagcttgag tttgtagaag
     2701 agctgatgat ggagggacta gcggaaggta taccgtttag tttgcccatt tgaataggga
     2761 gaacggtgcc accggcatcc attgctaggg gatcgtgaca taggcagcgg ccttagaatg
     2821 cacttgccgt atccgtccct tgcggtccag tggtgctcgt aacgcccgcg gtcgctgtac
     2881 cgttaaggca acccgcaaca tcataaatcg tggtgccgct gacatttccg ttatctcggc
     2941 caacggtgga tgagccctga tgtggccctg ctcaaccgct acaaaagcga tacagctcaa
     3001 ggctgcctca aggcgagggg agcctggttt ctgttcctgt cgtccgatta aacggacgac
     3061 gctctaagcc aggctttctt ccaatgccct cttgaaatcg cgtaactcct caggattgag
     3121 gtctgaaatg gcggcgtaag agaagcctcc tcgcgtccag ctcaggaggt gataaccatc
     3181 cgcggtttcc ggaatggctt gctgttttcc tgcgggccag acgaaaagat tgatgacatg
     3241 gccgtggcgc ttgtaaacga tagcggccac tacccggccg ccgatgtagt cgaggcggcc
     3301 accgatcagc ggaaagtctt tcttcgacag gtccacgacg ggaggcgcga agtcgagctt
     3361 tccgaggaac cagggcttga cggtgtgctg atcagaagaa accacatcgg tcaagtgcga
     3421 agccagcaaa gatctcacat gcccggcaac aagttcgtcg tcaaggct
//
LOCUS       DATMGX010000626         2285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_116373, whole genome shotgun sequence.
ACCESSION   DATMGX010000626 DATMGX010000000
VERSION     DATMGX010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116373"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..2253
                     /locus_tag="VNR65_10210"
     CDS             73..2253
                     /locus_tag="VNR65_10210"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWJ19087.1"
                     /translation="MENVNLESENRSELHFDGIAGGSEAVRRVVNEIKTVAPTDSIIR
                     ISGENGNRRDALYRAVMALSRSIAGCTDLRSLLAGAAESVRQIVSFDHVALILHDSNS
                     DTMQGYILNEPCNPVITSLRLPVDQDPAGWVWLNQRPLVVTSVQSETRWPEFVSRARD
                     FNITTLMLVPLTTGDRRLGAFGFGSVAHLEPSPAEIAFLERVASEFAVAVESFLAKQA
                     AVRERDRLRTLFDITDALVSKLDRDELFSAISDQLSKVIRHDSALLTICNETGSLDVY
                     ALHSTIPQLLQPLKGPFNPVGMPAEEVLASGKPVIAGEADVDRYPNPNFRRFLELGYK
                     SICSVPLIARNRIIGTLALNRMTDAPWTSEEVEFLVQVAGQVAMTVSNSLAFRELGEI
                     KERLATVKLYLEDEIRLDQNIGNMIGEGPGFQAVVNGIQTVAPTGATVLITGETGTGK
                     ELVARAIHDLSGRGKGSFVKVNCAAIPASLLESELFGHEKGSFTGAVAQKIGRFELAH
                     GGTLFLDEIGEMPLELQPKLLRAIQDQEFERVGGNRTIRTDARLVAATNRDLKAMVDD
                     NKFRADLYYRLHVFPLHVPPLRDRREDIPLLTRYFIQKHAHRMGRNIDTIPTCVLDAL
                     TNYHWPGNIRELQNVLERSVILTHGSALQVPMTELTGKTIPTTLTGTPAAHPHHAERG
                     TILKALEEARGQVGGPDGAAARLGLKRTTLQSRMRKYNISREFS"
BASE COUNT          489 a          688 c          685 g          423 t
ORIGIN      
        1 gctccacacg atttcgcagc aagagcgtgg agtcgcagga ggcgtcgtgg gctgcgaaaa
       61 ggaggatagg tcatggaaaa cgtgaatctg gaatccgaga atcggagcga gctccatttt
      121 gacggaatcg ccggcggcag cgaagccgtg cggcgcgtgg tgaacgagat aaagacagtg
      181 gcgccgacgg attccatcat ccggatcagc ggcgagaacg gcaaccgccg ggacgcactc
      241 tatcgggccg tcatggcgct ctcgcgttcg atcgccggtt gcacagacct gagaagtctg
      301 ctggcgggcg cggccgaatc ggtccgccaa attgtcagct tcgatcatgt cgcgctcatt
      361 ctgcacgatt ccaacagcga caccatgcaa gggtatatct tgaatgagcc ctgcaatccc
      421 gtaatcacaa gccttcggct gccggtcgac caggatcccg ctggttgggt atggctaaac
      481 cagcggccac tcgtcgtcac atctgtccag tcggaaactc gctggccgga gttcgtcagt
      541 cgcgctcgcg acttcaacat caccactttg atgcttgtcc ccctgactac gggggataga
      601 cggctgggcg cgttcggctt cggctccgta gcgcatctcg agccaagccc ggccgagatc
      661 gcgttcttgg agcgcgttgc cagcgagttc gccgttgccg tcgagtcctt cctggcaaag
      721 caggcggcag tccgggagcg ggaccggttg cgaaccctgt tcgacatcac cgatgcgctt
      781 gtgtcgaagc tggatcgaga cgagctattc tctgcgatat ccgaccagct ctcgaaggtc
      841 atccgccacg atagcgcgct actcacgatc tgcaatgaga ccggcagcct cgatgtatac
      901 gcgctgcatt ccaccattcc gcagcttctt cagccattga aagggccctt caaccctgta
      961 ggcatgccgg cggaagaggt gctcgccagc ggaaagcccg taatagccgg cgaagccgat
     1021 gtcgaccgct atcccaatcc aaacttccgg cgttttctgg agctcggcta taaatccatt
     1081 tgctcagttc cactgatcgc acgaaatcgt attatcggca cgctcgcgct caatcgaatg
     1141 acagacgctc cctggacttc cgaggaagtc gagttcctgg tgcaggtcgc gggccaggtt
     1201 gccatgacgg tatcgaactc actcgctttt cgagagctgg gagagatcaa ggagcggctc
     1261 gcgaccgtga agctctacct cgaagacgag atccgcctcg accagaacat cgggaacatg
     1321 ataggcgagg ggcccggttt tcaagccgtc gtgaacggca ttcagaccgt ggccccgacg
     1381 ggtgcaacgg tcctcatcac gggcgaaaca ggcacgggaa aagaactcgt tgcgcgtgcc
     1441 attcacgact tgagcggccg cggtaagggc agtttcgtaa aagtgaactg tgcggcgatt
     1501 cccgccagcc tgctcgagag cgagctattc gggcacgaga agggatcgtt caccggagcc
     1561 gtggcgcaga agatcggtcg cttcgaactg gctcacggag gcacgctgtt cctcgacgag
     1621 atcggggaaa tgccgctgga actccaaccg aagctactgc gtgccattca ggaccaggag
     1681 ttcgagcgtg tgggcggcaa tcgcacgatc cgcaccgacg cgcgcttggt cgccgccaca
     1741 aatcgggatt tgaaggcgat ggtggacgac aacaagtttc gcgccgacct gtactatcgc
     1801 ctgcatgttt tcccattgca cgttccgccc ttgcgggacc gccgggaaga tataccgttg
     1861 ctgacgcgct acttcatcca gaagcacgcg caccgcatgg gccgcaacat cgatacgatt
     1921 cccacgtgcg ttttggacgc actcacgaac taccactggc cgggcaacat ccgcgagttg
     1981 cagaacgtgt tggagcggtc cgtcattctg acccatggga gtgcccttca agtgcccatg
     2041 accgagctca cgggcaaaac cattcctact acgttgaccg gaaccccggc ggcgcacccg
     2101 caccacgcgg aacgggggac gatcctgaag gcgctggaag aagcaagagg ccaggtcgga
     2161 ggtccggacg gcgccgcggc tcgactggga ttgaagcgaa ccaccctgca atcccgcatg
     2221 cgcaaataca acatctcacg cgagtttagt tgagccaacg gaacctgcat caagcatcag
     2281 gaagt
//
LOCUS       DATMGX010000627         3064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_163435, whole genome shotgun sequence.
ACCESSION   DATMGX010000627 DATMGX010000000
VERSION     DATMGX010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163435"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(685..1023)
                     /locus_tag="VNR65_10215"
                     /pseudo
     CDS             complement(685..1023)
                     /locus_tag="VNR65_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1384..2577
                     /locus_tag="VNR65_10220"
     CDS             1384..2577
                     /locus_tag="VNR65_10220"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HWJ19088.1"
                     /translation="MPDKSAKGIDRRDLIMASIAAVGGAASVAVNAGAANAQAAAIPS
                     GNPASGTIYTGDVIEGKKVVSALDVNELEPGKKHLLYFQGVEMPTGQHWYVSVTVAKG
                     AKPGKRGILTSGVHGDEMSSVHTVQTVMNQLDPAQMSGTVMAVTDVSRPALESMQRRW
                     PNQGRGIDLIDMNREWPGNENGATAPSRHAGLLFNRLLRPNADFAIDFHTGTTGFKVT
                     AFNIGGMDVPDVKAMVELYPVGQIFDNHVYPGVLHNAFMDVGIPSFTPEIGAARVLDP
                     QMISLFVEGTMNVLKHHGIVAGPMGRTGKDVTVFVGNSAYPILATAGGLVEHLVNLND
                     KVEAGQKVAIQRDSFGKVVAEYASGVAGAITGQRSDAMSEPGNPLVFILFNKPGQEDV
                     QVYLE"
BASE COUNT          644 a          887 c          907 g          626 t
ORIGIN      
        1 ctcccggcgc gatactccag ctggtcagtg tcactccggc aaccgcaatg atggagccag
       61 gcaggatatc tgtcagcatc ttgcggcggt tcacggtttc cactatgtct tgttaccttg
      121 tcctaaaaat tcccggggct atcagtccaa ccacaactta catccgcgtt ggaccactcg
      181 ccccaaaggc cttcgttaga tcgttgagag acgacgtcgg ttaaactaaa agaaggataa
      241 cgctggaaag cagggagaag caaatgttgc gaaccatatt tgcgatgacc tttgcgggtc
      301 tggttctcgc ggccattcct ggcactgccc aggctgcacc gatcgccccg attacgacag
      361 gtatcgccgc tgaggtgagc gatgtgttgg acgtcgcatg gcgccggtgt tggcgagacc
      421 gctggggccg cgtgcattgc cgtacatgtt ggcgcgatcg ctggggccgc gtgcgctgca
      481 ggtgattgtg ggcgccgttg cggcggcgat tacttcaaac ttaggatcag tcgccgcggg
      541 acggttcaaa gacgaggtca ataaatgcaa cggtttgctc aggtcgtcat gatcctcgct
      601 tccttggcgg ctctggtgtc caccgttcag ccggcgaccg cgggaaagta ctgtttgcag
      661 ggaaggcaat ggggctttcc ggagccggtt ctccgagaac gtcgagtggt gcgggatcct
      721 gtcattaaga tcgagcctgc agaaccagcg gtaggccaga tgtagctcaa cttcctgggt
      781 caacttgcgc tccgagcgaa ggccgtagca atagccaact atcagcatcc ggatcatcag
      841 ctcggggtct atcgagggcc gaccgatctc gctgtaataa ggagccaatt gctggtgcat
      901 gccgcccaaa acaggcgtca aatatagatt gatccgcctg agtaaatggt ctttcgggat
      961 acgatcatcg agccgaaact cgtagaacaa gctggtttga tcaccggact gccgacccat
     1021 catcgccgcc tccgacgcag tggtcccatg aatcaccgga atcactgttc ggagatattc
     1081 tcaagccttt ttcgacagca tcgaccccat gcagacatcg ctcggcgcgg cgggggaact
     1141 tgatagatac cttccatcct actctgtcgc cctactagct gggccacaag gaaagagttg
     1201 atccagatca acatgactcg aacgagagcg gttagctatc ccgcaacctt gcgagttcag
     1261 tggcatacgc gcgtatttgg aagggccgcg cgggattttg tgaccgatgt gcataccgag
     1321 ctgtcgtacg aacgttgcct ttcacgatga cagttgcctt tcacaatcac taaggacccc
     1381 atcatgccgg acaagtctgc aaaaggcatc gaccgccgcg atctgataat ggcatccatt
     1441 gccgcggtcg gcggcgcggc ttccgtcgcc gtcaacgctg gtgctgcgaa tgcccaggcc
     1501 gcagcgatac cctctggcaa tccggcatca ggaacaatct ataccggcga tgttatcgaa
     1561 ggcaaaaagg tcgtcagcgc gctggacgtc aacgaacttg agccgggcaa gaagcacctt
     1621 ttgtattttc aaggcgttga gatgccaacc ggacagcact ggtatgtgtc tgtgacggtc
     1681 gctaaaggag caaagccggg caagcgtggt atcctgacca gtggcgtgca tggcgacgag
     1741 atgagttctg tgcatacggt ccagacggtg atgaaccaac tcgacccggc gcagatgtcg
     1801 ggcacggtga tggcggtcac ggacgtgtcc cgccctgccc tggaaagcat gcagcgcaga
     1861 tggcccaacc agggcagagg catcgatcta atcgatatga accgggagtg gcccgggaac
     1921 gagaacggcg ccaccgcacc cagccgacac gccgggcttc tcttcaaccg gctgctgcgg
     1981 ccgaacgccg acttcgcgat cgacttccac acagggacaa ccggattcaa agtcaccgca
     2041 ttcaacattg gcggaatgga tgtgcccgac gtgaaggcga tggtggagct ctatcctgtc
     2101 ggccaaatct tcgacaatca tgtgtatccc ggtgtccttc acaacgcgtt tatggacgtg
     2161 gggatcccgt ccttcacacc ggaaatcggc gctgcgcgcg tcctagaccc ccagatgatc
     2221 tcgctcttcg tggaaggcac aatgaacgtc ctcaagcatc acggcatcgt tgccgggcca
     2281 atgggacgta caggcaagga cgtgactgtt tttgtcggta acagcgcgta ccctattctg
     2341 gccaccgcgg gtggtctcgt tgagcatctg gtcaatctca acgacaaggt cgaagccgga
     2401 cagaaggttg ccatccagcg cgatagcttc ggcaaggtgg ttgcggagta tgcaagcggc
     2461 gtggccgggg cgataacggg ccagcgcagc gatgcgatgt ccgagcccgg caacccgttg
     2521 gtattcatcc ttttcaacaa gccagggcag gaggacgttc aggtctatct cgagtagttg
     2581 ccgcatcgaa ctctcgtcct tgatcaatgt cacctattcc gcagcactgc ggctggactc
     2641 aacccactgg cctgcgccga cgaggctgaa cgatgtcagc tcctggcaca ctgaacggac
     2701 ctgagcgtgt cgcctgacga tgtctgtttc cgggatggca tctctcactg acgggcaaaa
     2761 aggggggcaa gaagtgaaag gcgttgccgc gctgtatagc ctatcgaatt cgtaggccaa
     2821 gaagtcttgc cacatttggc cccggcgggg gccgtcggtc gcacctgttt gcgtcacggc
     2881 tcacgccttc cccggccctg accgggggag gcgtatggtg gtcgtcaatg acgcattgca
     2941 gtcgccgata agtcgctcag ttaccgttcc tcacgccgct cgtggcgtcc ttcacgccgc
     3001 ccatgacgcc gctggcggcg tccttcacgc cgctcacgac gccgctggcg gcgtgaagga
     3061 cgcc
//
LOCUS       DATMGX010000628         3048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_388705, whole genome shotgun sequence.
ACCESSION   DATMGX010000628 DATMGX010000000
VERSION     DATMGX010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388705"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VNR65_10225"
     CDS             complement(<1..326)
                     /locus_tag="VNR65_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19089.1"
                     /translation="MLSIDAVQQANSGHPGPPMALAPLVYTIWNRLLRFDPKDPIWPD
                     RDRFLLSNGHASMLLWSVLHLTGTRAVNAEYERLGQPSVTLDDIRHFRPFGASAPLKE
                     LQKKFG"
     gene            complement(377..1099)
                     /gene="pgl"
                     /locus_tag="VNR65_10230"
     CDS             complement(377..1099)
                     /gene="pgl"
                     /locus_tag="VNR65_10230"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWJ19090.1"
                     /translation="MKIEVFADPDGAAREAARFVAAEARKAVADRGQFAAAFSGGHTP
                     WVMLRALADEDVPWDKVHIVQVDERVAPSGDRDRNLTHLRENLLDHAPLSPDHIYAMP
                     VEENDLTAAAGQYDLILQMLAGTPPVLDLVHLGLGPDGHTASLVPGDPVLDITSADVA
                     VTRAYQGHPRMTLTYPILNRSRRLLWLVTGAEKAGMLVRLINGDSSIPAGRVHQDVAL
                     VLADRAAADQLGQIADRSRDHE"
     gene            complement(1187..2833)
                     /gene="pgi"
                     /locus_tag="VNR65_10235"
     CDS             complement(1187..2833)
                     /gene="pgi"
                     /locus_tag="VNR65_10235"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HWJ19091.1"
                     /translation="MPMIKESAPWKALEDHQKKMRDVSLRDLFADDPRRGERMTAEAA
                     GIFLDYSKNRITDETLKLLIDLAEQSGLRARIDAMFRGEKINVTEGRAVLHVALRAPK
                     SETISLDGQNVVPEVHAVLDKMADFSRRVRDGHWKGHSGKRIKTIVNIGIGGSDLGPV
                     MAYEALKHYSDRALTFRFVSNIDSTDLVEATIDLDPEETLFIVASKTFTTLETMTNAH
                     SARDWLLKGFGGDVNAVSKHFVAVSTNASKVAEFGIDTANMFGFWDWVGGRYSMDSAI
                     GLSTMLAIGPEHFRAMLDGFHEMDEHFRTTPFERNLPVLMALLTVWYTNFFGAETIAV
                     LPYEQYLKRFPAYLQQLTMESNGKHVTLDGVEVDYQTGPIYWGEPGTNGQHSFYQLMH
                     QGTRLIPCDFIAFEQPLNPLGRHHDMLLANVFAQAEALAFGKTSEEVKAEGTPDWLVP
                     HRTFEGNRPSNTLLMDRLTPAALGRLIALYEQNVFTQGVIWDIDSFDQWGVELGKVLA
                     QRIIPELEGQPGTELRHDTSTNDLIDRYRNAKAAHGRNPV"
BASE COUNT          592 a          898 c          921 g          637 t
ORIGIN      
        1 aagccgaatt tcttttgcag ttccttgagc ggagcggagg cgccgaaagg acggaaatgg
       61 cgaatgtcgt cgagcgtgac ggatggttgc ccgagccttt catattcggc attgaccgcg
      121 cgcgttccag tcaggtgaag cacggaccac aacagcatcg atgcgtggcc gttcgacagg
      181 aggaaacggt cgcggtccgg ccagatcgga tccttgggat cgaaccgcag caagcgattc
      241 caaatcgtgt agacaagcgg cgcgagcgcc atcggcggcc caggatgtcc tgagtttgcc
      301 tgctgaacgg cgtcgattga gagcatgttc ggcggtcatc gaactcgcgg gatttttctg
      361 aaacgcctca gcggtctcac tcatggtctc gactccgatc cgctatctgg ccgagctggt
      421 cagccgcagc ccggtcagcg agcacgaggg ccacgtcctg gtggacgcgc ccagccggga
      481 tcgatgaatc gccattgatc aggcgaacca gcatccccgc cttctcggcg cctgtcacga
      541 gccacagaag tcgccgggaa cggttcagga tcggataggt cagggtcatt cgcggatgcc
      601 cttgataggc gcgggtcacg gcgacatccg cgcttgtgat gtcgaggaca ggatcgccgg
      661 gcacgagcga agcggtgtgg ccgtccggcc cgagcccgag atgaacgagg tcgagcaccg
      721 gcggcgtgcc ggctagcatt tgcaatatga gatcatactg gccggccgcc gcggtgagat
      781 cgttttcttc gaccggcatg gcatagatgt gatcgggaga cagcggagca tggtcgagca
      841 ggttctcgcg caaatgcgtg agattccgat cgcggtctcc tgagggtgcc acccgctcgt
      901 ccacctggac gatatgaacc ttatcccagg gcacgtcttc gtcggcgagc gcgcgcagca
      961 tcacccatgg tgtatgcccc ccgctgaacg cagcggcgaa ctgcccgcga tcggcaacgg
     1021 ctttccgagc ttctgctgca acgaagcgag ccgcctcgcg ggcagctcca tcagggtcag
     1081 caaagacttc gatcttcatg acggcctccc gatcgggatg ggactttcgt ggactcttcc
     1141 cccttctaac ttccgacatt ccttaaaggt cccccgattg tctccgtcaa accgggttac
     1201 gaccgtgagc cgcctttgcg ttgcggtacc ggtcgatcag atcatttgtc gaagtgtcat
     1261 gacgtagttc cgtgcccggc tgtccttcga gctctggaat gatgcgctgc gcaagaacct
     1321 tgccgagttc cacgccccac tgatcgaacg agtcgatgtc ccagatgacg ccctgggtga
     1381 acacattttg ctcgtaaagc gctataagcc ggccgagtgc ggccggtgtc agccggtcca
     1441 tgagaagagt gtttgacggc cggttgcctt cgaaggtccg gtgaggaacg agccagtccg
     1501 gcgtgccttc cgctttcacc tcttcgctcg tcttgccaaa ggcgagcgcc tccgcctgcg
     1561 cgaagacatt tgctaagagc atgtcgtgat ggcgtccgag cgggttaagc ggctgctcga
     1621 aggcgatgaa atcgcacggg ataagccgcg tcccctggtg catcaactgg tagaacgagt
     1681 gctggccgtt ggtgcccggt tcgccccagt agatcgggcc ggtctggtaa tcaacctcga
     1741 cgccgtcgag cgtcacatgc ttgccgttgc tctccatggt gagctgctgg agataggcag
     1801 ggaagcgttt caaatactgc tcgtagggca ggaccgcgat ggtctcggcg ccgaagaagt
     1861 tcgtgtacca gaccgtcagc agcgccatca gcacaggcag attccgctcg aacggagtgg
     1921 tgcggaagtg ctcgtccatc tcgtggaagc catccagcat ggcgcggaaa tgttcggggc
     1981 cgatggcgag catggtcgaa aggccgatcg cggaatccat ggaatagcgg ccgccgaccc
     2041 agtcccagaa tccgaacata ttggccgtgt cgatgccaaa ctcggcgact tttgaagcgt
     2101 ttgtcgacac ggcaacgaaa tgcttggaca ccgcattcac atcgccgcca aatcccttca
     2161 gcagccagtc gcgcgcgctg tgcgcgttgg tcatcgtttc cagcgtcgtg aatgtcttcg
     2221 aggcgacaat gaaaagcgtt tcctcgggat cgagatcgat ggtcgcctca acaagatcgg
     2281 tgctgtcgat gttggagaca aagcgaaagg tcagggcgcg atcgctgtaa tgtttcaggg
     2341 cctcgtacgc catgacgggc ccgagatcgg agccgccgat gccgatattg acgatggtct
     2401 tgatgcgctt tcccgagtgt cccttccaat ggccgtcacg gacacgcctc gaaaaatcgg
     2461 ccattttgtc gagcacggca tgcacctccg gtacgacgtt ctggccgtcc agcgaaattg
     2521 tttcgctctt cggcgcacga agcgcgacat gcaaaacggc ccgtccttcg gtcacattga
     2581 tcttctcgcc ccggaacatg gcgtcgatcc gcgcgcgcag gccggactgc tccgccagat
     2641 cgatcaggag cttgagcgtt tcatccgtga tgcgattttt cgagtagtcc aggaaaatgc
     2701 cagccgcctc agcggtcatc cgctctccac gtctgggatc atccgcgaag agatcgcgca
     2761 gactgacgtc gcgcattttc ttctgatgat cttcaagggc cttccacggc gcgctttcct
     2821 tgatcattgg cataccaccg gtcctgtctt gtattgagct aaccagcttt ttccagcatc
     2881 gtggacttgg atgcaataat gcctatcagc tcatgccagg atttcacgag ggacttcgct
     2941 cctgtcgagc ctcaggctct gctcgggatc gtaaggtgat tgagtgcgct catgatgcca
     3001 gagtccttca gcctttgcga tagagcaagt atcgcgactc tcaacgtt
//
LOCUS       DATMGX010000629         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_320597, whole genome shotgun sequence.
ACCESSION   DATMGX010000629 DATMGX010000000
VERSION     DATMGX010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320597"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(648..1046)
                     /locus_tag="VNR65_10240"
     CDS             complement(648..1046)
                     /locus_tag="VNR65_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ19092.1"
                     /translation="MLLARVIGIGIETADMLVREILSRKLRDRRALARYAGLTGSPDE
                     SGLKRREKGLAKAGNARVRRGLIQLAWRFLMFQKDSALARWYRTRTEGPSGARKTTMI
                     VALARKLLIALWRLVTTGEVPDGVELRPAA"
     gene            complement(1885..>2064)
                     /locus_tag="VNR65_10245"
     CDS             complement(1885..>2064)
                     /locus_tag="VNR65_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19093.1"
                     /translation="GRHNERRSLKGTQQFRPGPSAGDGSPMTVRGGGDPMDSMAYHAV
                     TENGSAASELRNRCV"
BASE COUNT          334 a          707 c          577 g          446 t
ORIGIN      
        1 ccgccttgcg ctctctagcc caaataccac gccgcaccgc attaggaaga cggggtctca
       61 tcaggtcccg gccactttcg cggtagggac gcgagttacc tcgcgccccc cgcacagatc
      121 cgtacgggcc caattcgggc atacggcttg tatggggctt tctttgtcaa gggggcgtca
      181 cgccattttg acgtcgtcct ttgcattcca ttcttttgtt cgacccgcgc cgcgagcatt
      241 cttccgtccc gaccgcatat ttccatcgcc gtcgcgcgca gcggcggtca aggacggcgc
      301 ttcttcagcg ccgccgaagg cttgtccttg acggacgcga gcacggcggc aggctgccgg
      361 cgatcgggtg gttggccgcg cgatgatccg aggggagcct gccattggcc gcgatcaatc
      421 gcgcgaacca cgcttgtatt cggttgaacc cgtatggggt cccgaccatg aagcagtcat
      481 acgcttctgc gggcaaggct ccgaggcggc gggtccattc tctcagcggc attgcagcca
      541 tagccgaatg cggattgccg ccacctcgga tcgtcatcgg ctacccggcg catcaagtgc
      601 ggtcaggtgc cgaaccctgt ttgtcctttc tcgttctaga gtgttgctca cgccgccggc
      661 cgcagctcga caccgtccgg cacctcgccg gtcgtgacca ggcgccacag cgctatcagc
      721 agcttgcgcg ccagtgccac gatcatcgtc gtcttgcgcg cgccgctcgg cccctcggtc
      781 cgcgtgcgat accagcgcgc cagcgcactg tctttctgga acatcaggaa gcgccacgcc
      841 aactggatca gcccacgtcg tacccgcgca ttgccggcct tggccaggcc cttctctcgg
      901 cgtttcaagc cgctctcgtc gggcgacccc gtgagcccgg cgtagcgcgc cagcgctctt
      961 cgatctcgaa gcttccgcga caggatctcc cgcaccagca tatccgccgt ctcgatgccg
     1021 ataccgatca ccctggcgag cagcaatgcg ctgcttgtcg tccggtttgc atccgccctt
     1081 cgtgcacctc gtagtcacga cgtttgtccc gcactgcgtc gggccgtgga ctcgataatg
     1141 taccgccgac cgccccagag gccctcgctc gggtccgggt tatagtgtcc cggtccgtca
     1201 tcacttaatc ggcctcatcc gccccactcg caggcacatc gcattttcac cgcatggcgg
     1261 cttatacgcg atgccttcgc tgtgcgggag cgccgaggcg acccgcgagt ggttccgggc
     1321 tttcactgca cattccttcc tggcatgccg tcctctccga ccccggggag ttcgacatcg
     1381 atatccagaa acttcgatgc cgacattggc cttcgccgaa tgaccaccgg ctcggcactc
     1441 ccgacactcc cgcaatccgt tccgcgcggg tttccggatt tcgtggcttc actggttcgc
     1501 ttccgctacg gcctgccagg ttgctcgctc ccctgtacgg atcagactgg atcgcccagc
     1561 cttcggggac ttttacttcc aggctttcaa cggatcagtc tccctctccg ttgctggata
     1621 taactacaac agcgactgga ctccttgtat ggggcttttt atgtcaagaa gtttaagaag
     1681 ggcagcgcat ctttttcctt tcatcagttt cgttgttcga tttgaaagtt cgtttcttcg
     1741 tcctgacccg ccacggcggc accatgcaaa tgcagtcggc tgggtcagca aaacgacggt
     1801 cagaggggag cccgcgttcg agcgcggatc tatcgcgcca caaccttgta ttcggtgggt
     1861 ccaagaaccc gaaccatgat gcagtcatac gcatcggttg cgaagctccg aagcggcgga
     1921 cccattctct gttacggcgt gataagccat ggagtccatc gggtcaccgc cacctcgaac
     1981 cgtcatcggc gacccgtcac cagcggacgg accgggccga aactgttggg tgcccttcag
     2041 tgacctccgt tcattatgcc ggcc
//
LOCUS       DATMGX010000630         9979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_279595, whole genome shotgun sequence.
ACCESSION   DATMGX010000630 DATMGX010000000
VERSION     DATMGX010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279595"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1283
                     /gene="gap"
                     /locus_tag="VNR65_10250"
     CDS             258..1283
                     /gene="gap"
                     /locus_tag="VNR65_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWJ19094.1"
                     /translation="MALKIGINGFGRIGRLVFRAAADNPNIEIVGINDLVPSDNLGYL
                     LKYDTTHGRFAGTVEAKEDGLVVNGKHVPCYSVRNPAELPWGKNGADYVIESTGLFTD
                     YAGAEAHLKAGAKRVVLSAPTKDPEKVKTLLMGVNHTKFDPATDKVVSNASCTTNCLA
                     PVAKVINDNFGLTEGLMATIHAVTATQPTQDGPSRKDWRGGRNGYMNIIPSSTGAAKA
                     VGLVLPELKGKLTGMAFRVPTTDVSVVDLTFKTAKATSYKEICAKMKEASEGAMKGIL
                     GYTDEEVVSSDFIHDAHSSIFDAGAGIELNANFFKVVSWYDNEWGYSTRVVDLITHMA
                     KKDGLLK"
     gene            complement(1699..2256)
                     /locus_tag="VNR65_10255"
     CDS             complement(1699..2256)
                     /locus_tag="VNR65_10255"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEMT/PEM2 methyltransferase family protein"
                     /protein_id="HWJ19095.1"
                     /translation="MLLLTHLIFDVAFAALVILNVLLLLTVFSSSIKLWPTPSTKSWQ
                     HYTFWPLFRGGLGLTLLYAILTAHSPQDGGALRFLIGMICVVVGFGFTIYGYFDLGIE
                     NTYGADEGLVTKGLYRYSRNPQYVASILGFFGTALANGETHAVILCGLAILVYVIMPY
                     TEEPWLQRAYGDVYLAYKERTPRFL"
     gene            complement(2521..3522)
                     /gene="pip"
                     /locus_tag="VNR65_10260"
     CDS             complement(2521..3522)
                     /gene="pip"
                     /locus_tag="VNR65_10260"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HWJ19096.1"
                     /translation="MRHDVFHVALTGSMTNRRHLFPEIEPYRTGWLKVSNTHDIYFEE
                     CGSPLGKPVLIVHGGPGGGCNPAMRRYHDASRYRIILFDQRGCGRSTPHACLEENTTW
                     DLVADIERLRMHLGIGAWQLCGGSWGSTLALAYAEAHPDRVTELLLRGIFLLRKAELD
                     WFYQEGGSWIFPDAFEQYAAVIPSAERGNMIEAFHRRLTSGNREIELAAARAWSIWEG
                     TTLSLFHDQERVQRFGSDSYALAFARIECHYFINKGFFRSDNQLIEDAGRIADIPGTI
                     IHGRYDVVTPLKNAWDLAKAWPKAELRIVPDSGHAMTEPGIIHEIVTATDKYASAPK"
     gene            complement(3605..5479)
                     /locus_tag="VNR65_10265"
     CDS             complement(3605..5479)
                     /locus_tag="VNR65_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19097.1"
                     /translation="MTLNYSTPVINPRLGSSFAIFASAYTCLVVMLIVLEQLGLSVPT
                     IDELIIVTPALFYIAIGFMTRTTSTDDFLLAGQRVPPFYNALAFTSMVFGGSIMLGSI
                     GSLFFLGIDAIAIPIGCFAGVVLTGVLFVPHLRKAGANTLPGFLHLRFGHRGVRLAAA
                     ILMLVPCLIILVAEAALGGKLLGFFLPLPQTLGIKLSPPAFFLMLVLASVVLTVALGG
                     MRSATWTQCVQFVVVLGILAPLIAVSVIRTKLPLPQLTYGSQLEEIKAREQTKGFVTV
                     TQPQPLSDVLPKPAAQPVIRPVERAFSAFRPMDFLLLVLCLATGVAAHPAFFPRLSMT
                     PTIPASRRSMGWVTVIAGFVVLTIPAYAFFTKAMVVEALVGVPASDLPAWARALQQLG
                     LINLPANQFDSFNAAAQRVTFHRDSLALILPVAGNLPRVFFGLAAASALAALSACAAG
                     HLVAVANTVSDDVYHAFLNKTASPGRRLMVARLTMLLFAVAVFIITQNSAADPLRWAI
                     AAFCLSSGTFFSVLLLSVWWRRLTSRGALAGLISGFVVTALYLRMGGTPLFGIDALTA
                     AALGVPVSFVAAVLVSLLSKAPEQSALEAIDDLRVPAGETLQSRMLRLAARAKPLGKV
                     "
     gene            complement(5481..5741)
                     /locus_tag="VNR65_10270"
     CDS             complement(5481..5741)
                     /locus_tag="VNR65_10270"
                     /inference="COORDINATES: protein motif:HMM:NF025305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/substrate symporter small subunit"
                     /protein_id="HWJ19098.1"
                     /translation="MSEDDIKQAYWRRIRRLTFGMIVVVAGLTLLLPIFMPTLNAYRF
                     LRFPLGYFLVGNAAILVLAILIYGFFGGQQRSDRAYNMTIQF"
     gene            5830..6579
                     /locus_tag="VNR65_10275"
     CDS             5830..6579
                     /locus_tag="VNR65_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ19099.1"
                     /translation="MPAAAIYVDEENAHEKALCSGENAMTAHHVIIADDHPLFRDALK
                     QTLATSLPGIGFTEVGSVEKLLDALSVHPNVDLVLLDLKMPGEKGAASLSGLTGLMYL
                     RSQHPEIPVVIVSASDNPSMIRRCANLGAAGFISKSLPVDKIRLAVKTVLDGGVWFSD
                     EMQREGGDELGDLAARLATLTPQQVRVLSMLGQGLLNKQIAYELNVSEATVKAHVSAI
                     LQKLGVDSRTQAVIAVNRIAAVDDHADEPRV"
     gene            7038..7676
                     /locus_tag="VNR65_10280"
     CDS             7038..7676
                     /locus_tag="VNR65_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ19100.1"
                     /translation="MATGDASFGAYKPVRSAVELTTLELALPPSARDLQAKIEECQVC
                     HQPSGQGFRGVPPVPRLAGQQIEYFVNQLRAFIEHRRVHPIMVNVAQFLSPAMQRALA
                     EHFRSLNPRPVGGVRKELAGSGKKIYEEGVPDADVPPCAVCHGPEAKGNGPFPRLAGQ
                     LHDYILSKLENWNQERGQNPAKQDTSAIMQPIAHNLTKAQMEAVAAYLNYLE"
     gene            complement(7821..9305)
                     /locus_tag="VNR65_10285"
     CDS             complement(7821..9305)
                     /locus_tag="VNR65_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWJ19101.1"
                     /translation="MGQPKASNPLVFLSCLALATACSPASLRAQDTNKDRTVQSSVRS
                     GAYTEAAALLETQARAGNAEAQYQLASLYRIGRGVAPNETEAFRWMQQAASAGHVRAQ
                     YSLASMYLGGRGTPVDFKAAEMWAQRAADKGHDKARDILAEIAAKRSAPKEVPAWKAA
                     ASDDPQAISGRAAAVSSDTGKALIDAARRGNLEATKALIKRGAPLESRDDDGNTALGL
                     AAGAGHARIVEALLSAGAKANARNSSQETPLIRAASQNRGPEVELLAKISDVDAKRAD
                     NATALTLALRRCSEKTVNALLAAHANLNDGSGEPSPLSLASLGCGAGIVKAIMERGAH
                     VNEADSLGRTPLWHAADRGNVDAALVLMHGAGNPGQPDKNGVTAFLHALERGHAELAS
                     RMLAEGADPNQATASGNTPLMLAAKLGSAPLVEALIRAGAKVDARNAFGNSALMIAAS
                     EGQTAAARTLLAAGADRSLRNKKREKAVDVASAAGHSDLAEVLK"
     gene            complement(9292..>9979)
                     /locus_tag="VNR65_10290"
     CDS             complement(9292..>9979)
                     /locus_tag="VNR65_10290"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWJ19102.1"
                     /translation="VAHFPRRKLVMTQPAELPLAGKMNFTEVYKEELLEFWTKVVRDE
                     KLRIRYGEQVEAIERTATGFEVRTGRGTFKSRAVLLAIGRRGTPRRLDVPGEERSKVV
                     YRLTDPEQYAGQHVLVVGGGDSALEAAGSIALETDAKVTLSYRSEAFSRAKPKNRTRV
                     DQAIKSGRLNLMLKSQVAEIGESHVDLEWQGKRLRMKNDAVIVCAGGILPTNFLKSIG
                     VEIETKYGTA"
BASE COUNT         2215 a         2866 c         2932 g         1966 t
ORIGIN      
        1 ttgaacgaac gccttcgtca gaacagcctg cccgagaagc tgacgaggct gtggatcgac
       61 cgcgcgctca agaagatcgg cgagcggccg gcgattgcag cggaatagcg gtgcagttcg
      121 tcccttacat ggcaattttt cggaacatgg ctctcagtct tccggcgtgt tggccagtcg
      181 tcttgtctgc ttgacatcat ccgcgccccg tggccctttt tgcgggggac ttgagctaca
      241 cacaaggagg acgtcctatg gcattgaaaa tcggtatcaa cggatttggc cgtatcggcc
      301 ggttggtatt tcgcgcagca gcggacaatc cgaacatcga gattgtcggc ataaacgacc
      361 tcgtgccctc cgacaatctc ggctatctgc tgaagtatga caccacccac ggcaggttcg
      421 ccggcacggt cgaggcgaaa gaagatggct tggtcgtgaa cggcaagcac gtgccgtgtt
      481 actcagtcag gaacccggcc gagcttcctt ggggaaagaa tggcgcggat tatgtgatcg
      541 aatcgaccgg cctgtttacg gattacgccg gcgcggaagc gcatctcaaa gccggcgcga
      601 agcgagtggt tttgtcggcg ccgaccaaag acccggaaaa ggttaagacg ctcttgatgg
      661 gcgtgaacca caccaagttt gacccggcca cggacaaggt cgtttccaat gcaagctgca
      721 cgacgaattg tctcgcgccg gtcgcaaaag tcatcaacga caatttcggc ctgaccgaag
      781 ggctgatggc gacgatacac gcggtgactg cgacgcagcc gacgcaggac ggcccgagcc
      841 ggaaggattg gcgtggcggt cgaaacggtt acatgaacat catcccctcc agcacaggcg
      901 ccgccaaggc agttggtctg gttctcccgg agctgaaagg caagctcacc ggcatggcgt
      961 tccgcgttcc gacgaccgac gtctcggttg tggatctcac attcaaaacc gcaaaggcca
     1021 cgagctacaa agaaatctgc gccaagatga aggaagcgag cgaaggcgcg atgaagggca
     1081 ttctcggcta cacggacgag gaagtcgtca gcagcgattt catccacgat gcgcacagca
     1141 gcatcttcga cgccggggcc ggcatcgagc tgaatgcgaa cttcttcaag gtcgtgagct
     1201 ggtacgacaa cgaatggggc tacagcacgc gagtcgtcga tctgatcaca catatggcga
     1261 agaaggatgg gctgttgaag taaggtccct ccatctgaca tcggcctcgc cgcagtacgg
     1321 acagccagaa accgcctaaa taggtaatga cattcgcgtc ttgcggatgg cggtggaatg
     1381 gctgactgcg gtggcaatct taagacttaa ccttgcgcgc agcgccgaag tctgttagtt
     1441 aggcggagat ccattgccct gacagggtgg atttactgac cctggctctt acaagagcga
     1501 gggttttttt tattgtcgac cgtgaacttc tgattttttt gcagccgctt cacaagcgcg
     1561 gaatcgtcgc cggtggcgcc atagttactg ccgcttattc cactctcggc ctgttgggtg
     1621 tatcgcgccc tttcaacgtc ggtcgatcaa ccggctcgca cagcggcgtt aaatatttgg
     1681 ggccgcgcgg cgtccgcctc aaaggaagcg cggcgtccgt tccttgtagg cgagataaac
     1741 atcgccatac gctcgctgaa gccacggctc ttccgtgtaa ggcatgatga cgtagactag
     1801 gatcgcgagc ccgcagagga tgacggcgtg tgtttcgccg ttcgccagtg ccgtgccgaa
     1861 aaagcccaga atggacgcca catattgcgg gttgcggctg taccggtaga ggcccttcgt
     1921 cacgaggccc tcgtccgcgc cgtaagtatt ctcgatgcca agatcgaagt agccgtatat
     1981 cgtgaagccg aagccgacca caacgcaaat catcccgatc aagaagcgca gggccccacc
     2041 gtcttgcggc gagtgtgccg tgaggatcgc gtaaagcagc gtcaatccaa gcccgccgcg
     2101 aaaaagcggc cagaacgtgt aatgctgcca gctcttggtc gagggcgttg gccaaagttt
     2161 gatcgagctt gagaagaccg tgagaaggag aagcacgttc aggataacga gcgcagcaaa
     2221 agccacgtca aagatgaggt gcgttagaag caacattgat aaagccggtt ataaaatatt
     2281 gtcggactca cgccctgcgg caattctatt aggtctcaca gggtgcgagc gcgggtcaac
     2341 cccccactcc taagccgcgt cgaaattatt cggaatcccc ttattgtcat ggccgccctc
     2401 cgaggcggcc atccataaca aattccatct tttttggatc ttgaggctgg attggtcggg
     2461 gcaagcccgg ccatgacatc ggttcaagct aatcgcaaca ggttttagga taaaaagccg
     2521 ctacttcggg gccgaggcgt atttatcagt tgcggtcaca atttcatgga tgatgcccgg
     2581 ctccgtcatg gcgtgcccag agtcgggtac gatgcggagt tccgctttcg gccaagcctt
     2641 ggccaagtcc caggcgtttt tcagcggagt cacgacatca taacggccgt gaatgatcgt
     2701 gccggggata tccgcaatcc ggccggcatc ctcgatgagc tgattgtcgc tgcggaaaaa
     2761 gcccttgttg atgaagtagt gacattcgat ccgcgcgaag gcgagcgcat aactatcgct
     2821 gccgaacctc tgcacgcgct cctgatcatg aaaaagcgaa agggtggtgc cctcccaaat
     2881 gctccacgcg cgggctgcag ccagctcgat ttcgcgattg cctgacgtca agcggcggtg
     2941 gaaggcttcg atcatgttgc cgcgttcggc ggaggggatc accgcagcgt actgctcgaa
     3001 ggcatcaggg aaaatccagc tgcccccctc ttgatagaac caatcgagct cggccttccg
     3061 caggaggaag atgccgcgca agagcagctc ggtgacgcgg tccggatggg cctccgcgta
     3121 ggccaaagcc agcgtcgagc cccaggagcc cccgcaaagc tgccacgccc cgatgccgag
     3181 atgcatgcgc agccgttcaa tatcggcaac gagatcccag gtggtgtttt cttccaggca
     3241 cgcatggggt gtggagcgcc cgcagccgcg ctggtcgaaa aggatgatcc ggtatcgcga
     3301 cgcatcatga taacgccgca tggcaggatt gcagccgccg cctgggccgc catgaacgat
     3361 cagcacaggc ttcccaaggg gcgagccgca ttcctcgaaa taaatatcgt gcgtgttcga
     3421 aaccttgagc caaccggttc ggtacggttc aatttccggg aataggtgcc tgcggttcgt
     3481 catggagccg gttaaggcta cgtgaaagac atcatggcgc atgcaaaggg cctcaaggcg
     3541 ttaccccaca gaaaacgctc tccgggatat aatagatgta acgtcgcccc agccgatagc
     3601 ctagttatac tttgccgaga ggcttcgccc gcgcggctaa acgcagcatc cgcgattgaa
     3661 gcgtttcccc ggcggggacc cgcaggtcat caatcgcttc cagcgcagat tgctccgggg
     3721 ctttggacaa aaggctgacg agaactgcgg caacgaagct gacgggaacg ccaagcgccg
     3781 ccgccgtcag cgcatcgatc ccgaaaagcg gcgttccccc catccgcaaa taaagcgcgg
     3841 tgacaacaaa gccggaaatc aatccggcta aagcgcccct tgacgtcaat ctccgccacc
     3901 agacactcaa gagaagaacg gaaaagaaag tcccggagct gagacagaag gcggcgatag
     3961 cccaccggag cggatcggcc gccgagttct gcgtgatgat aaagaccgcc acagcgaaaa
     4021 ggagcatcgt cagacgagcc accatgagac gccgccctgg agaagccgtc ttgttcaaaa
     4081 acgcatgata gacgtcgtcg ctgaccgtgt tggcaaccgc aacgagatga ccggcggcgc
     4141 atgcggagag ggcggcaagc gccgatgccg cggcgagacc gaagaaaacc ctaggcagat
     4201 ttccggccac cggcaggata agggcaaggc tgtcccgatg aaacgtcact cgttgcgccg
     4261 cggcgttgaa ggaatcgaat tgattcgccg gaagatttat cagcccgagt tgctgaagcg
     4321 cccgcgccca tgctggcaaa tctgaagctg gcacgccaac aagcgcctca acgaccatcg
     4381 ctttcgtaaa gaaggcatag gccggtattg tgaggaccac aaatcctgcg atcacggtta
     4441 cccagcccat ggatcggcgg ctcgccggaa ttgtgggtgt catgctcagc ctggggaaga
     4501 aagcggggtg cgcggcgacc cctgttgcca gacacaacac caggagcagg aaatccatcg
     4561 gcctgaatgc gctgaaggct cgctcgacgg gccgaataac gggctgcgct gccggctttg
     4621 gcaggacgtc gctgagcggc tgcggctgcg taaccgtcac aaaacctttc gtctgctctc
     4681 gcgcctttat ctcttcgagc tggcttccgt aggtaagctg cgggaggggc agtttcgtcc
     4741 gaataaccga gactgcgata aggggcgcga gaatgccgag aacaacgacg aattggacac
     4801 actgagtcca cgttgcggaa cgcatcccgc caagagccac cgtgagaacg acgctggcga
     4861 ggacgagcat caggaagaat gccggaggtg aaagttttat accaagcgtt tggggcagcg
     4921 gcaggaaaaa gccgaggagt tttccgccga gcgcggcttc cgcaacgaga ataataaggc
     4981 acggcacgag catcaggata gcggcggcga ggcgaacccc ccgatgtccg aaccgcaaat
     5041 gaaggaatcc gggcagcgta ttggctccag ccttccttaa gtgcgggacg aacaaaacgc
     5101 ctgtcagcac aacacctgca aaacacccga tagggatcgc aatcgcatcg atccctagga
     5161 aaaagaggct gccgatgctg ccgagcataa tgctgccgcc gaacaccatc gaggtaaagg
     5221 caagcgcgtt gtagaaaggc gggacgcgct gccccgcaag cagaaaatcg tcggtgctgg
     5281 tggtgcgcgt catgaagccg atggcgatat agaacagcgc gggcgtaacg ataatcagtt
     5341 catcgatcgt cggcaccgaa aggccgagct gctccaagac gatgagcatc acaaccaggc
     5401 aggtgtaggc gctggcgaag atcgcgaagc tggaaccgag ccttggattg atgaccggcg
     5461 tgctgtaatt aagcgtcata tcagaattgg atcgtcatgt tgtaggcgcg gtcgcttcgc
     5521 tgctggccgc cgaagaagcc ataaatgagg atcgcaagca ccaaaattgc agcgttgcca
     5581 acaagaaagt atccaagtgg gaagcgcagg aacctgtaag cgttcagcgt cggcatgaaa
     5641 atcggaagaa ggagagtcag tcccgcgacg acgacaatca taccaaatgt aagccgccga
     5701 atgcgccgcc aataggcttg cttaatgtcg tcttcagaca tacctttgga atctcaagtg
     5761 aactttcatt gaatcttggg aatcgatcct gctatcctac tccagtcccg ggatgagaca
     5821 aagcctgccg tgccagcggc ggcaatctat gtggacgagg agaacgcgca tgaaaaggct
     5881 ctctgttctg gtgagaacgc gatgaccgcc catcatgtca tcattgcgga cgaccatccg
     5941 ctttttcgcg atgctttgaa gcaaacgctg gcaacgtcgc ttccgggcat cggcttcacg
     6001 gaggtgggat cggtcgagaa actgctcgac gcgctttccg tgcatcccaa tgtcgacctc
     6061 gtgcttctcg acctgaagat gccgggtgaa aagggtgcgg cgtcgctctc cggcttgacc
     6121 gggctgatgt atctcagaag ccagcacccc gagatcccgg tggtcatcgt ctcggcgagc
     6181 gacaacccca gcatgatccg gcgctgcgca aacctcggcg cggccgggtt tatctcgaaa
     6241 tccttgccgg tcgacaagat ccgcctggcc gtgaaaaccg tcctcgacgg cggcgtctgg
     6301 ttctccgatg agatgcaaag ggagggcggc gacgaacttg gcgatctcgc ggcgaggctc
     6361 gcgacgctga ccccgcaaca agtccgggtg ctgtccatgc tcgggcaggg cttgctcaac
     6421 aagcagatcg cttacgagtt gaacgtctcc gaagcgaccg tgaaggcgca cgtctcggcg
     6481 atcctgcaaa agctcggggt cgacagccgg acgcaggccg tcatcgcggt gaaccgcatc
     6541 gcggcggtgg atgaccacgc cgacgagccg agggtttagt gaagtatctt taataaaagt
     6601 ttatcctgat ggcacaggtg aaaagcgcat agctaaaaag gttggcgaga aattcgatat
     6661 gaattgctat gagagaactt tgcgcgaatt tactatttag ttgatgaccg ctatctccga
     6721 taggtagcga ggcggagacc aagcgttcct ctcaactatc aagggttaga gcgggctgga
     6781 gctttcgtta acctgccggc aaggcgattt cgctgaagat ctttcagaag aaggcatgcg
     6841 gacgaaatcg tcagggacaa gttgacaagc agatgcggcc ttgcgcaaca tgcatacaat
     6901 catccagacg cccgcgttcc cattaaaggc gcgatgtctt tagacaaaaa ttgtgcaacc
     6961 aatacggttt tcatttgcat tattatcgaa taacgtattt tagccttata atcgggttgg
     7021 ctttcgcgag ccccgctttg gcaacgggtg acgcttcttt tggcgcttac aaacccgttc
     7081 gatccgctgt cgaactcaca acattggaac tcgcgctccc tccctcggcg cgagaccttc
     7141 aggctaagat cgaagaatgc caagtttgcc accagccttc ggggcaaggc tttcgtggcg
     7201 ttcctcctgt accacgcctc gcaggacaac agatcgaata ctttgtaaac cagctacggg
     7261 cctttattga gcatcggcgc gtgcatccta tcatggtcaa cgtggcgcag ttcctcagtc
     7321 cggcgatgca aagggctctt gcggagcatt tccggagtct caatccgagg cccgttggcg
     7381 gagttcgaaa ggaactcgcg ggctctggga aaaaaatcta cgaagaggga gttcccgatg
     7441 ccgatgtgcc tccctgcgct gtctgtcacg gaccggaggc caaaggtaac ggaccatttc
     7501 ctcgtttggc aggtcagctc catgactaca tcctcagtaa actggaaaat tggaaccagg
     7561 agcgtggtca gaatccggca aaacaagata catcagccat catgcagccg attgcgcaca
     7621 acttgaccaa ggcacaaatg gaagccgtcg cggcctatct caattatttg gagtaaagcc
     7681 ttgcctttac accgggtctc ctttccttgg agcgtgcggc gctcttaact cctccttcaa
     7741 ggcgaacccg acgaaccacc tttgcggcga tcttgcgcag caggtgaaac tgccatttga
     7801 cattctgaag atgcgggctc ctatttcagc acctcagcca aatcggaatg cccggcggcg
     7861 ctcgcaacat cgacggcttt ctcgcgcttc ttgttgcgca ggctgcggtc cgctccggct
     7921 gccagcagag tccgagctgc cgccgtctgc ccctcgctcg cagcaatcat caaggcgctg
     7981 ttgccgaatg cattcctcgc atcgactttg gcgcctgccc tgatcaaagc ctcgaccagc
     8041 ggcgcagaac caagtttcgc ggcaagcata aggggcgtgt ttccagaagc cgtggcttgg
     8101 ttcgggtcgg cgccttcggc taacatgcgc gatgccaact cggcgtgacc tctttccaac
     8161 gcatgcaaga aggcggtgac accgttcttg tcgggctgcc cgggattgcc tgccccgtgc
     8221 atcagtacta gagcagcatc cacgtttccc ctgtccgcgg catgccaaag gggcgtccgg
     8281 ccgagcgagt cggcctcgtt tacatgggcg ccacgctcca taatagcttt cacgatgccg
     8341 gcaccacaac cgaggctagc gagcgaaagg ggtgacggct cgccactacc gtcgttcaga
     8401 tttgcgtgcg ccgcaagcaa agcattgaca gtcttttcgc tacatcggcg aagcgcaagt
     8461 gtcagggcgg tcgcgttgtc agcacgtttt gcatccacat ccgaaatctt ggccagaagc
     8521 tccacctcgg gcccacggtt ctgggaggcc gcccgtatca atggcgtttc ctgggagcta
     8581 ttgcgcgcgt ttgccttggc gccagccgac aagagcgcct cgacgatgcg agcgtgcccc
     8641 gcgccagcgg caagtccaag ggcggtgttg ccatcgtcgt cgcgagattc gagcggcgct
     8701 cctctcttga taagagcttt tgtggcttcg agattgccgc gacgggccgc atcgatcaaa
     8761 gcttttccgg tatccgaact cacagcggcc gcgcggccag atattgcttg cggatcgtcc
     8821 gatgcggcgg ccttccaagc gggcacttcc ttcggcgcag atcgtttcgc agcgatctcg
     8881 gcgaggatat cgcgagcctt gtcgtggcct ttgtctgcag cgcgctgcgc ccacatctcc
     8941 gcagccttga aatcaaccgg cgtcccacgt ccccccagat acatgctggc aagactgtac
     9001 tgcgcccgga catgccccgc actcgctgct tgctgcatcc agcgaaaggc ttcagtttca
     9061 ttcggggcca caccccggcc aatccggtaa agagatgcga gttgatattg cgcctccgca
     9121 tttcctgccc gcgcctgcgt ttcgagaagg gcggcggcct ccgtataggc tcctgaacga
     9181 accgaggact gcaccgtacg gtctttattt gtgtcttgcg cccgtaggct tgctggcgag
     9241 catgccgtcg caagggcaag gcaggagagg aaaacgagag gattgctggc tttaggctgt
     9301 cccatacttc gtctcgatct ctacaccgat ggattttagg aagttcgtcg gcagaatgcc
     9361 gcccgcgcag acaattaccg catcgttctt catgcgcagg cgcttgcctt gccattcgag
     9421 gtcgacatgg ctttcgccga tctccgcgac ctggcttttg agcatgaggt tcagtcggcc
     9481 cgacttgatg gcctgatcga cgcgtgtgcg gttcttgggt ttggctcgcg agaaggcctc
     9541 ggagcggtaa gacagtgtca ccttcgcatc tgtctcaagc gcgatgctgc ccgcggcttc
     9601 gagagcgctg tctccgccgc cgacaacgag cacatgctga ccggcatatt gctcggggtc
     9661 tgtaaggcgg tatacgactt tggaccgctc ctcccccggg acatccagcc gtcgcggagt
     9721 accgcgcctg ccaatggcga ggagcacggc gcggctctta aaggttccgc gacctgtgcg
     9781 aacctcaaaa ccggttgccg tcctttcgat tgcctcgacc tgctctccat aacggatcct
     9841 gagtttctcg tcgcgaacga ccttggtcca gaactcgagc agctcttctt tatagacctc
     9901 agtgaagttc atcttgcctg cgagaggcag ctctgcgggc tgtgtcatca caagtttgcg
     9961 gcgtgggaaa tgcgcgacc
//
LOCUS       DATMGX010000631         6624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_116473, whole genome shotgun sequence.
ACCESSION   DATMGX010000631 DATMGX010000000
VERSION     DATMGX010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116473"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VNR65_10295"
     CDS             <1..297
                     /locus_tag="VNR65_10295"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWJ19103.1"
                     /translation="CHCFDINDEVTGKSPLTGARVRTWDNCQFPDFTMHSSGHNPRED
                     TGARLRQRICHKLLYFVENHKMQQCTGCGRCITHCPVGIDIVKIANVMEEGVWQ"
     gene            294..1118
                     /locus_tag="VNR65_10300"
     CDS             294..1118
                     /locus_tag="VNR65_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HWJ19104.1"
                     /translation="MSENAYLPQLARIVNIKEEVPGARAIRTFRVQFLNGGGFDHEPG
                     QCAMISVFGKGESMISIASAPVITDYKQFSIMRSGRVTTAIHDMKVGDVIGVRGPYGN
                     KFPVNDWKGRNLVFIGGGCGLAPIWPVIQTAVTNRKDFGTITVFYGGRTSRDIMYAAE
                     LEKLRGAADVHLSIDSPEEGWEGYCGFVPANVMTKPQSPDNTIAITCGPPIMIKFVIN
                     NLREMGFADDQIYTTIENKMKCGIGKCGRCNVGKDYVCVKGPVYSWAQLKTLPQEY"
     gene            1149..2423
                     /locus_tag="VNR65_10305"
     CDS             1149..2423
                     /locus_tag="VNR65_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HWJ19105.1"
                     /translation="MNLAGMLYGAELLKSVDFPTSDVLGPEASDAEIKALIVKCGEIF
                     IKPNFRGGVGKKGKAGLVGRAKDLRTAKKEKERLYFVEHCHGNAVAKAHGVTFEGAVP
                     AEHEVYVCIADSTRYRAPTITITHRGGVEIEDLPKDQIAEIPFDPLTGLKSFVVTNAL
                     TDIKAPKEIISPLAQVLPKLWDLFHYNGMTTLELNPIRMMPTGKGGLTPVACDFKCGF
                     DRDDPRVKRLNLPKQLFVEDYTDFEYEINQLRTYQGQSDVSVINANGTILAPVFGGGA
                     NSLVTEFLGDDAIISSDFGGNPPYDKMREVMRICLKHWLKQSNVLFIIGGKANNTDIF
                     VTFRAMADALREYFSEHGPTPVYVVIGRGGPNLARGMLAFKDVCDLIGLPYRFFGFDS
                     AMTDVVRYAQAADRWMKNGGREMLAHKLGVAP"
     gene            2481..5177
                     /locus_tag="VNR65_10310"
     CDS             2481..5177
                     /locus_tag="VNR65_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19106.1"
                     /translation="MNQGIERFKYFVGIRSLADIATREDRVCVLNILGNESRAVAPTS
                     HAYSRGNIVFGTSPGRRGEVLETPIGPIPVYNNVRDGLDDGHNFNVGVIHLPPAAVRD
                     GAHELMRVNKHVQKIIILTEKVPVHDSREIRAMAQQRHVDLFGPNCLGVADAWNHVRI
                     GGPLGGERPEESLRKGSVAIYSNSGNFTTTIASYLASSGWGTTTSISSGKDVYIGFAP
                     AEFACAFDYDSRSKAAVMYIEPGGYYEEDLEFSKPVIACVVGRWKAKLTRAVGHAGAM
                     GGDGDSAEAKEKWFQRILNVDGIFTPERPIISRAGAVVTNISHIPAALTAVMKLHGVR
                     LDFAPEGDLTLKPWFGNNQGLVLPPELDIPLIEPIQPYKDQISELSKQVGTIFPREAL
                     KDRSGSSIMDAKTQVTRVSGVSILDTAKYPLESNYCLALTHEHNDDNDNALFNTAIAA
                     EVNLNETAVKVADAARESGSAPNTVLASACALAGPKTVAGARKAADVLIELFGPSGLK
                     TGDEEGFDFSGIQANAGQLAALVSKSPDTKGAAMLTGLKARGAKSVFVDYLHSLNGYP
                     TADAVLAAITTSISWGALMKKKVTRNTVRNFPWFARLFATIIGASVESSRHKENTFCG
                     VPVSDMINSWTATEMAYLALLGETPTPEKLFPFQVLLGLIISNGPGTISAQGCKGAVS
                     ADGPETPERVQINKAMIGFLTHTGFSHGGNGFEGMRFLISQFGKTDLKDPGDPNHGLD
                     LAAMAGRFADEFAANKKARKGVGGDVQAIPGVHHPIFRNQTINKDPRELFLSQLFKER
                     GESNVFHDFYSALVQALYDHGVSPNVFCVNIDAVIAALLLKTLWPRYRSGAFSEEALE
                     SAAFTAFLFGRTVGCAGEIDDHINRGRNMDTRTPASQCSYVV"
     gene            5210..>6624
                     /locus_tag="VNR65_10315"
     CDS             5210..>6624
                     /locus_tag="VNR65_10315"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWJ19107.1"
                     /translation="MTKHEPKIVWTMVDEAPALATYSLLPIVQAFTRVAGIDVETRDI
                     SLAGRILANFPENLTPAQQQSDDLAFLGELAKTPEANIIKLPNISASIPQLKAAIKEL
                     QGKGYDIPDYPEEAKTDAEKDIKTRYARTLGSAVNPVLREGNSDRRAAPSVKQYARAH
                     PHKMGAWSADSKTHVAHMSGGDFYGNEKSITVSEAGSFRIEFLGDDGHATVLKEKTAY
                     KAGEVIDAAVMSKRALREFFDAEIHDAKKKGVLLSLHLKGTMMKVSDPIMFGHAVSTF
                     FKDVFAKHAEALQKIGFNPNNGLGDLYAKLSKLPEAERAAIEAGIKTVYETHPELAMV
                     NSDKGITNLHVPSDVIVDASMPAMIRDSGKMWGKDGKLHDTKAMIPDRCYATVYQAVI
                     EDCKKHGAFDPKTMGTVPNVGLMAQKAEEYGSHDKTFEIPAEGKVRAVDASGKVLLEQ
                     DVEPGDIFRMCQVRDAPIQDWV"
BASE COUNT         1445 a         2018 c         1935 g         1226 t
ORIGIN      
        1 tgccactgct tcgacatcaa cgacgaggtc accggcaagt cgccgctcac cggcgcgcgc
       61 gtacgaacct gggacaactg ccagttcccg gatttcacca tgcactcgtc gggccacaat
      121 ccgcgcgagg acaccggcgc gcggctgagg cagcggatat gccataagct tctgtatttc
      181 gtggagaacc acaagatgca gcaatgcacg ggctgcggcc gctgcatcac gcactgtccg
      241 gtcgggatcg acatcgtcaa aatcgcaaat gtcatggaag agggcgtctg gcaatgagcg
      301 agaatgccta tctgccgcaa ctcgcgcgca tcgtgaacat caaggaagag gtgcccggcg
      361 cgcgcgcgat caggacgttc cgcgttcagt tcttgaacgg cggcggcttc gatcacgagc
      421 ccggccaatg cgccatgatc tcggttttcg gcaagggcga gtcgatgatc tcgatcgcgt
      481 ccgcgccggt gatcacggac tacaagcagt tctcgatcat gcgctcgggc cgcgtcacaa
      541 ccgccatcca cgacatgaaa gtcggcgacg tgatcggcgt gcgcggtcca tacggcaaca
      601 aattcccggt caatgactgg aaggggcgca atctcgtctt catcggcggt ggttgcggcc
      661 tggctccgat ctggcccgtg atccagaccg cggtcacgaa ccgcaaggac ttcggcacga
      721 tcaccgtctt ctacggcggg cgtacctcac gggacatcat gtacgccgcc gaactggaaa
      781 agctgcgcgg cgcagcggac gtgcacctct ccatcgactc gccggaggaa ggttgggaag
      841 gctattgcgg cttcgtcccc gccaatgtga tgaccaagcc gcagagtccc gacaacacca
      901 tcgccatcac ctgcgggcca ccgatcatga tcaagttcgt gatcaataat ctgcgcgaga
      961 tggggttcgc ggacgaccag atctacacaa ccatcgaaaa caagatgaag tgcggcatcg
     1021 gcaaatgcgg gcgctgcaac gtcgggaaag attatgtctg cgtgaaggga ccggtctact
     1081 cctgggcgca gctgaaaacc ttgccccaag aatattgata aagaagaaca ttttggagaa
     1141 aagacgctat gaaccttgca gggatgcttt atggggctga gctcctcaag tccgtcgatt
     1201 ttccgacctc cgatgtgctt gggccggagg cgagcgacgc tgaaatcaag gcgctgatcg
     1261 tcaaatgcgg tgagattttc atcaagccca atttccgagg cggcgtcggc aaaaagggca
     1321 aagccgggtt ggtcggccgc gccaaggatt tgaggacggc caagaaggaa aaagagcgcc
     1381 tctacttcgt cgagcattgt cacggcaacg ccgtggcgaa ggcccatggc gtcaccttcg
     1441 aaggcgcggt cccggccgag cacgaggttt atgtctgcat cgcggattca acccgctatc
     1501 gcgcgccaac gattacgatt acacaccgtg gcggcgtgga gatcgaggac ctgccgaagg
     1561 accagatcgc ggagataccg ttcgatccgc tgaccgggct caagtccttc gtggtcacca
     1621 acgctttgac ggacatcaag gcgccgaagg agatcatctc gcccttggcg caggttctgc
     1681 cgaagctgtg ggaccttttc cattataacg gaatgacgac gctcgaactc aatccaatcc
     1741 gcatgatgcc gaccggcaag ggtggcctga cacccgtggc ttgcgacttc aaatgcgggt
     1801 tcgaccggga tgacccgcgc gtgaagcgcc tcaatttgcc gaagcaattg ttcgtcgagg
     1861 actacaccga cttcgagtac gagatcaatc agctgcgcac ctatcagggc cagtcggacg
     1921 tttctgtcat caacgcgaac ggcaccatcc tcgcccccgt ctttggcggc ggcgccaact
     1981 cgctggtgac ggaattcctt ggcgacgatg ccatcatttc atcggacttc ggcggcaacc
     2041 cgccctacga caaaatgcgc gaggttatgc gcatctgcct caagcactgg ctgaaacagt
     2101 ccaacgtgct cttcatcatc ggcggcaagg ccaacaatac cgatattttc gtcacctttc
     2161 gcgcaatggc ggacgcactg cgcgagtatt tcagcgagca tggaccgacg cccgtctatg
     2221 tcgtcatcgg ccgcggcggt cccaatctgg cgcgcggcat gctggccttc aaggatgtct
     2281 gcgatttgat cggtttgccc tatcgcttct tcggcttcga ttcagcgatg accgacgtgg
     2341 tgcgctacgc gcaagccgcg gatcgctgga tgaagaacgg cggacgcgaa atgctcgccc
     2401 acaaactcgg tgttgcccca tgaaccgcgc cgcctttgat cgtttgaaac atttgcgaga
     2461 cggggttttt gggggttgct atgaatcaag gcattgagag gtttaagtat ttcgtgggca
     2521 taagatcgct ggccgatatc gctacgcgcg aggatcgggt ctgcgtcttg aatatccttg
     2581 gcaacgagtc acgcgccgtc gctccgacca gtcacgccta ttcccgcggc aacatcgtgt
     2641 tcggcacatc gccggggcgc cgcggcgaag tcctcgaaac gccaatcggc cccattcccg
     2701 tctacaacaa cgtgcgcgat gggctcgacg acggacacaa ctttaatgtg ggcgtgatcc
     2761 atttgccgcc tgcggccgtg cgcgatggcg cccacgagtt gatgcgcgtc aacaagcacg
     2821 tccagaaaat catcatcctg acggagaagg tgcctgtcca tgattcccgg gagatccggg
     2881 cgatggctca gcagcgccac gtcgatctct tcggcccgaa ctgtcttggc gtggctgacg
     2941 cctggaacca cgtgcgcatc ggcggtccgc tcggcggaga aaggcccgag gagtcgttgc
     3001 gaaaaggctc cgtcgccatc tattccaact ccggcaattt caccacgacc atagcgtctt
     3061 acctcgcgtc cagcggctgg ggaacgacga ccagcatttc cagcggaaaa gacgtctaca
     3121 tcggcttcgc gccggcggaa ttcgcctgcg ccttcgacta cgattcccga agcaaggccg
     3181 ccgtcatgta catcgagccg ggcggctact acgaggaaga ccttgagttt tcaaagccgg
     3241 tcatcgcctg cgtcgtggga cgctggaagg cgaaactgac gcgcgcggtg ggccacgccg
     3301 gcgcgatggg cggtgatggc gacagcgccg aagccaagga aaaatggttc cagaggatcc
     3361 ttaacgtcga cgggatattc acccccgaac ggccaattat ctcaagggcc ggtgcggtcg
     3421 ttacgaacat ttcgcacatc ccggcggcat tgaccgcggt gatgaagctt catggtgtgc
     3481 gtctggattt cgcgcccgag ggcgacctga cgctgaagcc gtggttcggc aacaatcaag
     3541 ggctcgtcct gccgcccgaa ctcgacatcc cgcttatcga gcctatccag ccttacaagg
     3601 atcagatctc ggaactctcc aaacaggttg gcaccatatt cccacgcgaa gccctgaaag
     3661 accgttcggg cagctcgatc atggacgcca agactcaggt cacgcgggtc agcggtgtgt
     3721 cgatcctcga caccgccaaa tatcctctcg aatcgaatta ctgcctcgcg ctcacccatg
     3781 agcacaacga cgacaacgac aacgccttgt tcaacacggc catcgctgcc gaggtcaacc
     3841 tcaacgaaac tgccgtgaag gtggctgacg cggcgcgcga gtcaggaagc gctcccaaca
     3901 ctgtcctggc ttcggcctgc gcactcgccg ggccaaagac cgtggcgggc gcacgcaagg
     3961 cggccgatgt cctgatagaa cttttcggcc cctccggcct gaagacgggc gacgaggagg
     4021 gcttcgattt ttcgggcatt caggcgaatg cagggcaact ggccgcgctc gtgagcaaga
     4081 gccccgatac caagggtgca gcgatgctga ccgggctgaa ggcgcgcggc gcgaagtccg
     4141 tattcgtgga ttatctccac tcgttgaatg gctatcccac cgccgacgcg gtgctggcag
     4201 ccattacgac gtcgatctcg tggggcgcgc tgatgaagaa gaaggtcacg cgcaacacgg
     4261 ttcgaaattt tccatggttt gcgcgcctct tcgcgacgat catcggagcc tcggtcgaga
     4321 gcagcaggca caaggaaaac accttctgcg gcgttcctgt cagcgacatg atcaacagct
     4381 ggaccgccac ggaaatggct tacctggcgc tgctcggcga aacgccgacg ccggagaagc
     4441 tcttcccgtt ccaggtgctg ctaggcctta tcatctccaa cggccctggc acgatctccg
     4501 cacaaggatg caagggcgca gtgtctgcgg atggtcccga gacgccggaa cgcgtgcaga
     4561 tcaataaggc gatgatcggc ttcctcacgc acaccggctt ctcgcacggg ggcaatggct
     4621 tcgaaggcat gcgattcctg atcagccagt tcggcaaaac cgacctgaag gatccgggcg
     4681 atcccaatca tgggctcgac cttgccgcca tggccggacg gttcgccgac gaattcgccg
     4741 caaataagaa agcgagaaaa ggcgttggcg gagacgtgca ggccattcct ggcgttcacc
     4801 acccgatttt ccggaaccag accatcaaca aagacccgcg cgagctcttc ctttcgcagc
     4861 tgttcaagga gcgcggcgag tcgaacgtct tccacgactt ctacagcgcg ctcgtccagg
     4921 cgctttacga tcacggagtc tcgccgaacg tcttctgcgt gaacatcgac gctgtgattg
     4981 cagcgttgct cttgaagact ctttggcccc gctaccgttc gggtgcattc tccgaggaag
     5041 ccctcgagag cgccgccttc accgccttcc tcttcggacg cacggttggc tgcgctggcg
     5101 agatcgacga tcacatcaac cgcgggcgca acatggatac ccgcacgcca gcatcgcaat
     5161 gctcctatgt cgtgtaaggt aggctgacac aatttttcga ggttaacgga tgaccaagca
     5221 cgaaccgaag atcgtctgga caatggtcga tgaagccccg gcgcttgcga catactcgct
     5281 tttgccgatt gtccaggcgt ttacgcgggt tgctggaata gacgtcgaga caagggacat
     5341 ctcgcttgcg gggcgtatcc tcgcgaactt ccctgaaaat ctgaccccgg cccagcagca
     5401 atccgacgat ctcgcattcc tgggagaact cgcgaagacg cccgaagcca atatcatcaa
     5461 gctgccgaac atcagcgcct ccattcccca gttgaaggcg gccatcaagg aactccaggg
     5521 caaaggctac gacatcccgg attatcccga agaggcgaag accgacgccg aaaaggatat
     5581 caagacgcgc tatgcgagga ctctcggcag cgccgtgaac cctgtcttgc gcgaaggtaa
     5641 ctccgaccgg cgtgccgccc cctcagtcaa gcagtacgcc cgcgcccatc ctcacaaaat
     5701 gggcgcatgg agcgccgact cgaaaacgca cgtcgcccat atgagcggcg gcgatttcta
     5761 cggcaacgaa aaatccataa cagtctccga ggcaggcagc ttccggatcg aattccttgg
     5821 cgatgatggg cacgcgaccg tgctcaaaga gaaaacggca tacaaagccg gtgaggtgat
     5881 cgacgccgct gtcatgagca agcgcgcctt gcgcgagttc ttcgatgctg agattcatga
     5941 cgcgaagaag aaaggcgtgc tgctgtcgct gcacctcaaa ggcacgatga tgaaggtctc
     6001 cgaccccatc atgttcggcc acgcggtttc cacctttttc aaggatgtgt tcgcaaagca
     6061 cgccgaagca ctgcaaaaga tcggcttcaa tcccaataat ggcctcggcg acctttacgc
     6121 caagctgtcg aagttgccgg aagcggagcg cgccgccatc gaagccggca tcaagaccgt
     6181 gtacgagacg caccccgaac tcgcgatggt gaattccgac aagggcatca ccaatctgca
     6241 cgtgccgagc gacgtgatcg tcgacgcctc catgcccgcc atgatccgcg attccggtaa
     6301 gatgtggggc aaggacggca agcttcacga cacgaaggcg atgatccccg accgctgcta
     6361 cgcaacggtc tatcaggcgg tcatcgagga ctgcaaaaag catggcgcct tcgacccgaa
     6421 gaccatgggc accgttccga atgtcggact tatggcgcaa aaggctgagg aatacggctc
     6481 gcacgacaag accttcgaga tcccggccga aggtaaggtc cgcgccgtcg atgcctcagg
     6541 caaggttctg ctcgaacagg atgttgagcc gggcgacatc ttccgcatgt gtcaggtgag
     6601 agacgccccc atccaggatt gggt
//
LOCUS       DATMGX010000632         4629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_218786, whole genome shotgun sequence.
ACCESSION   DATMGX010000632 DATMGX010000000
VERSION     DATMGX010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218786"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VNR65_10320"
     CDS             complement(<1..222)
                     /locus_tag="VNR65_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19108.1"
                     /translation="MCQAAPMSWQHVRISRFGGPEVLELAEESTIPNPGPGEVRIKVL
                     AAGTGFTDSFIRRGRYPDFKGPLPFTPGYE"
     gene            complement(482..658)
                     /locus_tag="VNR65_10325"
     CDS             complement(482..658)
                     /locus_tag="VNR65_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19109.1"
                     /translation="MAEYRVHIVDQDSNSRKTIRLECDNDDDAKGMANQLVNGLDVEL
                     WQRGRLIAKFQKSK"
     gene            complement(702..848)
                     /locus_tag="VNR65_10330"
     CDS             complement(702..848)
                     /locus_tag="VNR65_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19110.1"
                     /translation="MEKFIHDENLKLLRKCLAEATSDEERIVIRKLIAEKEAKNPRGT
                     KDRP"
     gene            complement(1129..1536)
                     /locus_tag="VNR65_10335"
     CDS             complement(1129..1536)
                     /locus_tag="VNR65_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19111.1"
                     /translation="MLALLFISDAYLPKLPVAERAYTDLPVIRIHSDRKWPERVVYDT
                     SLPTIIPAQIANTDASVPPPEAVADISAKAQVREAFAQLQPSDAEQLRQSKPKTPEPK
                     LQRKRKIAKRRVAPSMVLVARQPQFGFFGNSIW"
     gene            2646..3794
                     /locus_tag="VNR65_10340"
     CDS             2646..3794
                     /locus_tag="VNR65_10340"
                     /inference="COORDINATES: protein motif:HMM:NF006010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWJ19112.1"
                     /translation="MSIKGKAYLAGIYEHPTRHAPDKSTSQLHAEVAKGALEDAGLSK
                     ADIDGYFCAGDAPGGVMAMVDYLGLKVRHFDSTDTGGCSYLVHLGHAAEAIAAGKCSI
                     ALITLAGKPRTGAMPPRATGAEVDFEAAYGATTHNVYGMCAMRHMHEFGTTSEQLAWI
                     KVAASHHAQYNPHAMLKEVVTVEDVLNSPMISDPLHRMDCCVVTDGGGALIVTTEAIA
                     KSLKKPLVRMIGHGEALKGPRGGKDLNLTYSAGVWSGPRAFEEAGVTPKDIKYASIYD
                     SFTITVLMQLEDLGFCKKGEGGKFVADGNLISGVGKLPFNTDGGGLCSNHPVNRGGMT
                     KIIEAVRQLRGEAHPKVQVPNCDLAIAHGTGGLLGVRHAASTCILERV"
     gene            3794..4183
                     /locus_tag="VNR65_10345"
     CDS             3794..4183
                     /locus_tag="VNR65_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HWJ19113.1"
                     /translation="MDNPVKYPTPQGNPETKHFWDQAVEGKLLIKRCTACGEAHYFPR
                     AICPFCFSDKTVWEESSGEGEIYTFSLMRKSATGPYAIGYVTLKEGPSLLTNFVDCDQ
                     TKLKIGQKVKVVFKTTDGPPLPFFTPV"
     gene            4343..>4629
                     /locus_tag="VNR65_10350"
     CDS             4343..>4629
                     /locus_tag="VNR65_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19114.1"
                     /translation="MPIKYDELMALKNLGQKYAYGDREVMLYAYGIGMGANPMDEKEL
                     AFVNEAVATPRPLKVVPTFASVAAWGAGPGEMNLNRVMVVDGERDITFHKPL"
BASE COUNT          922 a         1410 c         1379 g          918 t
ORIGIN      
        1 ttcatagccc ggcgtgaacg gcagcggccc cttgaagtca ggataccggc cacgtcgaat
       61 gaagctgtcg gtgaaaccgg tcccggctgc gagaaccttg atgcgcacct cgccgggtcc
      121 tggattgggg attgttgact cttccgccag ttccaagacc tcggggccac cgaaccgcga
      181 tatgcgaacg tgttgccacg acatgggagc agcttggcac attcgactcc cgccagcgag
      241 attaatcggt tgtcagctgc tgcctttgga tgcctgtcgt cgctgtgggc cttgtcaccg
      301 ccgaaggata atcgacgtcg cttatgggtc tcaatcgctt cattttggcg accgaccagc
      361 cacttctggt ctaccccgat gaacggacat catcagaccg gcccggctgg tccgccaaga
      421 gcggaagtac gcactccgtc gtcccgatgg tttcaccctt gccctgatcc gtccgagcgc
      481 ttcattttga tttttggaac tttgctatca acctaccgcg ctgccaaagc tcgacatcaa
      541 ggccgttaac aagctggttc gccattccct tagcgtcgtc gtcattgtcg cattccagtc
      601 gaatggtttt tcgagaattg ctgtcttggt caacgatatg aacgcgatac tccgccatgc
      661 ttatatttcc gatggccgga cttcgctgtt cagaagcgtc atcacggccg gtcctttgtt
      721 ccgcgcggat tttttgcttc cttttccgcg ataagcttgc ggattacgat gcgttcttcg
      781 tcgctcgttg cctccgctag gcacttacga agcagtttga ggttctcatc atgaatgaat
      841 ttttccatga cggcgttccc tgacttcgcg tgtaacttgc aggccaccga tagaaggcgg
      901 tggtcattcc tagattttat ccggtcattc gtcttgggcg tcactgcacg tcgacccaaa
      961 cgaaagaggc cgtcaaccgg ggcggcctta ctttaagcgc ggcaattctc cagttatggg
     1021 ttttgcgaca tcgggcggcg gcaacgcgtt gttgccgtcg cagtcgaggc actggaactt
     1081 gcggccgccc aagtttttgc ggggtaagaa ccgtgccagt tagcctgctc accagatact
     1141 gttgccaaaa aagccaaatt gcggttgccg cgccaccaag accatcgacg gcgccacgcg
     1201 tctcttcgca attttacgtt tgcgttgcag cttcggctcc ggcgtttttg gtttggattg
     1261 tcgtagctgc tcggcatcgg atggttgcag ctgtgcaaat gcctcccgca cctgcgcctt
     1321 ggccgagatg tcggcgaccg cttccggggg cgggacgctg gcatccgtgt tcgcgatctg
     1381 cgcaggaatg atcgtcggga ggctcgtgtc gtaaacaacg cgctccggcc atttccgatc
     1441 agaatgaatg cggatgacgg gcaaatcggt atatgccctc tccgcaaccg gcaatttcgg
     1501 cagatacgca tccgaaataa acagcagcgc gagcaatact ccgccaacaa agaaaaaata
     1561 tcgcgccagg ggcattcgtc actccatccg acccctcgcg cgtactacag ggtttcagct
     1621 ggcaatcatt gatcgatatc aagcgccaat gaggtgtcta tttgtcttgc gtctgtacgg
     1681 tcccagactc agaaacatat cgatacggcg caccggtcgt tctgctcaaa atgcctgcaa
     1741 cgggtcaatt gcgccccggg cgagccagca gcaagtgcgg ccatgtccgc gacgccccga
     1801 aagcggaaat agctcaaagc atgagaggct tcgcaacggc tgcgcaccca ccgggcaacg
     1861 gttacggact gacttgcgcg gacgagctgc tcagccttcc tgcgcgagcg cgcggcgcgt
     1921 tgcgcgaaaa tccgatttgt cgaggcggat caacgtgatt tgctgtccag tccccttggc
     1981 aaaaagattt cgggttttcc gaaatgcaaa tcatcctatg tggtttgtca tcccgtcccg
     2041 aaagaggggc gtttgccatc gtcacgaacg ttgggacggg ttgcggtgga cgcggcagtg
     2101 cccgcgcgca aggtgtgatc gcagggcggg tcttaaccga tccctgagcg gtcggaccgc
     2161 gcgcagacga acggtgctgc ggcggacggc aaaagcgtgt ggtcctgacg cctcagcggt
     2221 tggcgtcaag tcttgcggag gttttgcgag cccgaccggg gcggacaaaa ccatattcgc
     2281 gaggcgatgg tgtcaaacaa gcccgatcac cagggagagc gcgctataag ccgtaaaacc
     2341 attgcgcagg gaatgccgga ttgcctccgc tgaacctgta tgctcgtgtg cgctttcttt
     2401 tgcacatctt gcacacgaga ccgcgggtgc agcgcgcacc cggcattccc tgcgccctct
     2461 ttttgcagag ggtaaggata ttccagcaaa ccccgggcgc atcgcgccgc gggacagtag
     2521 acgcacgccc gcattccgtg tcgcgggatt gcgctccctg gattttcggc agtcgccgct
     2581 tgccaagcct gccccggcag gccacaaaaa ccgccagcga aaaagaaacg ccagcggaga
     2641 acgacttgag catcaagggc aaggcctacc tcgccgggat ctacgagcat cccacccggc
     2701 atgcacccga taaatccacc tcacagctcc acgccgaagt cgccaagggc gcgctggaag
     2761 acgccggcct ctccaaggcc gatatcgacg gctatttctg cgccggcgac gctcccggcg
     2821 gggtgatggc gatggtcgat tatctcggcc tgaaagtccg gcatttcgat tccaccgaca
     2881 ccggcggctg ctcctatctg gtgcatctcg gccatgccgc cgaagcgatc gccgccggca
     2941 aatgctcgat cgcgctgatt acgctggctg gcaagccgcg caccggcgcc atgccgccgc
     3001 gcgcgaccgg cgccgaggtc gatttcgagg ccgcttacgg cgccaccacg cacaatgttt
     3061 acggcatgtg cgccatgcgt cacatgcatg agttcggcac caccagcgag caactggcct
     3121 ggatcaaggt cgcggcctcc caccacgcgc aatacaatcc gcacgcgatg ctcaaagagg
     3181 tcgtcaccgt cgaggacgtg ctcaactcgc cgatgatttc cgatccgctg caccggatgg
     3241 attgctgcgt cgtcacggac ggcggcggcg cgctgatcgt caccacggaa gcgatcgcga
     3301 aaagcctgaa gaagccgctg gtgcggatga tcggccacgg cgaggcgctg aagggcccgc
     3361 gcggcggcaa ggatctcaat ctgacctatt ccgccggcgt ctggtcgggt ccgcgggcgt
     3421 tcgaagaagc cggcgtgacg ccgaaggaca tcaaatacgc ctcgatctac gacagtttta
     3481 cgatcacggt gctgatgcag cttgaagatt taggcttctg caaaaagggc gagggcggca
     3541 agttcgtcgc cgacggcaat ctgatttccg gcgtcggcaa gctgccgttc aacaccgacg
     3601 gcggcggcct ctgcagcaat catcccgtca accgcggcgg catgaccaag atcatcgagg
     3661 cggtgcggca gttgcgcggc gaggcccatc ccaaggtcca ggttccgaac tgcgatctcg
     3721 ccatcgcgca cggcaccggc ggcctgctcg gcgtccgcca cgccgcatcg acctgcattc
     3781 tggagcgcgt gtgatggaca accccgtgaa atacccgacg ccgcagggca atcccgagac
     3841 caagcatttc tgggaccagg cggtcgaagg caagctgctg ataaaacgct gcaccgcctg
     3901 cggcgaggcg cattactttc cgcgcgcgat ctgcccgttc tgcttctccg acaagacggt
     3961 gtgggaggaa agctcgggcg agggcgagat ctatacgttc agcctgatgc gcaaatccgc
     4021 gaccgggcct tacgcgatcg gctatgtcac gctgaaggaa ggcccgtcgc tgctgaccaa
     4081 tttcgtcgat tgcgaccaga ccaagctgaa gatcggccag aaggtcaagg tggtgttcaa
     4141 gaccaccgac ggcccgccgc tgccgttctt tacgccggtg tgacgggggt tgtctctatc
     4201 tgcacagaga gatttatccg ctgagccaac ccctcaccac agcgaagttt gtggccggcg
     4261 ccggtgtagc cctctcccac aaggggagag ggcgcagcaa cgggcgccgg tagagaaaag
     4321 aagttcggga gaagatgaca agatgccgat caagtacgac gagctgatgg cgctgaagaa
     4381 tctgggccag aaatacgcct acggcgatcg cgaggtgatg ctgtatgcct atggcatcgg
     4441 catgggcgcc aaccccatgg acgagaaaga gctggctttt gtcaatgaag ccgtcgccac
     4501 gccgcgcccg ctgaaggtgg tcccgacctt tgcctccgtc gcagcgtggg gggcagggcc
     4561 cggcgagatg aacctcaatc gcgtcatggt ggtcgatggc gagcgcgaca tcaccttcca
     4621 caagccgct
//
LOCUS       DATMGX010000633         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_77922, whole genome shotgun sequence.
ACCESSION   DATMGX010000633 DATMGX010000000
VERSION     DATMGX010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77922"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VNR65_10355"
     CDS             complement(<1..424)
                     /locus_tag="VNR65_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWJ19115.1"
                     /translation="MVLIKSSRLNDQNIQGSALTSVVFGCVIIACLYFGREVLVPIAL
                     AVLMSFLLAPPVRLLQGWRVPRVLAVIVVVVVAFAGIFSLGGLMVGELNELARDLPRY
                     QLTLHEKIQSLRGATAVTGTLQRASEVLQELNSDLNMPK"
     gene            609..809
                     /locus_tag="VNR65_10360"
     CDS             609..809
                     /locus_tag="VNR65_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19116.1"
                     /translation="MNKLTKNNAAPPVSRSPARLPSSATGRVRAPCVRGGKTDASWIE
                     LVDHVVFGARPVVGLAIELMEA"
     gene            1302..2282
                     /locus_tag="VNR65_10365"
     CDS             1302..2282
                     /locus_tag="VNR65_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HWJ19117.1"
                     /translation="MSPHFLLSAAARSLSIGKVMRMSDQGAENVFARLRWPTTDGKPV
                     CPECGCTTCYDCRRGAYSRWRCKACGCDFSVTSGTLFAWRKLPLKTYLLSIVVFCNEV
                     KGKSMLALSRDLGVQYKTAFVLAHKLREAMASALRGLTIGGEGKVAEIDGAYFGGHVR
                     PENLAADRIDRRLAENQSGKRKVVVVMRERDGRTLPQVFSSEDAAVSFIRARIAKGTV
                     VHADESPAWNSLHAKFSMKRVNHQQEYSSDGACTNGAESYFSRMRRGELGHHHHIAGV
                     YFVRYAQEAAWREDHRGISNGDQVHGVIGLAMGLGPSVDFCGYWQRAKAA"
     gene            complement(2279..>2690)
                     /locus_tag="VNR65_10370"
     CDS             complement(2279..>2690)
                     /locus_tag="VNR65_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19118.1"
                     /translation="IISTLQDKRLEVADAIERLERQADQHRADLAHLEATMRLFDPNV
                     EPETVQSTPPRRRNEWFRPGECRRRIHDVLRDAARPMTTREIVEDVMAAKKLPDDDAR
                     TRELIHKTVLGSLNRATDTIERVEAMGSIAWRVI"
BASE COUNT          536 a          783 c          809 g          562 t
ORIGIN      
        1 gtttcggcat attgaggtcg ctgtttagct cttgcagcac ctcggacgcc cgttggagcg
       61 tgccggtcac ggccgtcgcg ccgcgtagac tttgaatctt ttcgtgcagc gtcaattgat
      121 aacgcggcag gtccctcgcc agttcgttga gctcccccac catcagccca cccaggctga
      181 agatccccgc gaatgcgacg accaccacga cgatgacggc gagaacccgc ggcacgcgcc
      241 agccttggag tagccggacc gggggggcga gcaagaagct catgagcacc gcgagcgcga
      301 ttggcaccag gacctcgcga ccaaaataga ggcaagcaat gattacgcag ccaaagacga
      361 cgctcgtgag cgccgatcct tgaatattct ggtcgtttag ccgtgaagat ttgatgagaa
      421 ccatttgggg gagatggtgc gaaagcgcga cggaggcaag gccgatcgaa gaactgccgc
      481 caaagtcagc gattggatta acctgggagc atcaactgag atagtgccga ccacagtgag
      541 tctcgacatt gcgcaaaatc gccgagcctc tacgagtcga agatttcgcc gatttccagg
      601 aaagggggat gaacaagctc acgaaaaaca acgcggcgcc tcccgtttcc cgcagccccg
      661 cccgccttcc gtcctccgct actgggcgcg ttcgcgcgcc ttgcgtccgc ggcggaaaga
      721 ccgatgcgtc ttggatcgaa ttggttgacc acgtcgtatt cggcgcgcgt ccggttgtcg
      781 gtctcgcgat cgaattgatg gaggcgtgaa ggtcggacgc gttccgcttc gcgcggtcgt
      841 tctcgcgatg ctgatcatct gagtatggca gattccggga ggcgtcgttt accatcaaca
      901 tcagctggcg cgaccttctg tcgcacgact cttcaccgcg ctgaatgttc cgaaaagatt
      961 tatgcgccgt taacctctga ggggcttcac tacggagtct ttgattgaca attcacatct
     1021 cgggacacac atagcgaaat atcgccgagg gctttcgttc tccgacgatt cagcaacctc
     1081 agaaagagac atgggcagta gcagctttgg cgccccgacc gcagctttgt ttgcgcgagc
     1141 gacgccgttc gaaatcgaaa ccgtccacgg tcgcggagac cgtgtgagag gcgtcgcgag
     1201 agcctaaagc tcggtcttat gtatcaaccg ccaccatcgc ttgacagcca tcacgatctc
     1261 gccgataacg ttcgtgttat gttcttgttg gcgagggtct catgtccccg cattttctcc
     1321 tgtcggcggc ggcgcggtcg ctcagcattg gcaaagtcat gcggatgtcc gaccaaggcg
     1381 ccgagaacgt tttcgcgcgc ctgcgttggc caacaaccga cggcaagcca gtgtgtccgg
     1441 agtgcggctg cacgacctgc tacgattgtc gtcgaggagc ctattcgcgt tggcgatgca
     1501 aggcgtgcgg ctgcgatttt tcagtgactt cgggcacgct gttcgcttgg cgtaaattgc
     1561 ccttgaagac ctatctcttg tcgatcgtgg tgttctgcaa cgaggtcaag ggcaagagca
     1621 tgctcgcctt gtcgcgggat ttgggggttc aatataaaac ggcgttcgtt ctggcgcaca
     1681 agctgcgcga agcgatggcc tcggcgttgc gtggcttgac gatcggcggc gagggaaagg
     1741 tcgcggaaat cgacggcgcc tattttgggg gccatgtccg accggaaaac ctggcggctg
     1801 atcggatcga ccgccggctg gccgagaacc agtctggaaa gcgcaaggtc gtcgtcgtga
     1861 tgcgcgaacg cgacggccgc acgttgcccc aagtgttctc ctccgaggac gccgccgttt
     1921 cgttcatccg cgctcggatc gccaagggaa ccgtcgtcca cgccgatgag agcccggcct
     1981 ggaattcatt gcacgccaaa ttctcgatga aacgggtcaa ccaccaacag gaatatagca
     2041 gcgacggcgc atgcaccaac ggtgccgagt cgtacttttc gaggatgcgc cgcggcgaac
     2101 tggggcatca tcatcatatc gcgggagtct atttcgtccg atatgcccag gaagcggctt
     2161 ggcgcgaaga tcatcgcggc atcagcaacg gagatcaggt ccatggcgtt atcggattag
     2221 caatgggcct tgggccatcg gtcgattttt gcggttattg gcagcgggcg aaagccgcct
     2281 aaatcacccg ccaagctatg ctccccatcg cctcgacgcg ttcgatcgta tcggtcgccc
     2341 ggttcaacga gccgagcacg gttttgtgaa tcaattcgcg cgtgcgagca tcgtcgtccg
     2401 gcagtttctt cgccgccatg acgtcttcga cgatttcgcg cgtcgtcatc ggtcgcgctg
     2461 catcacgcag gacgtcatga atgcgccggc gacattcgcc tggacgaaac cactcgtttc
     2521 gcctgcgcgg tggcgtcgat tggactgttt cgggctcgac gttcggatca aacagccgca
     2581 tggtcgcttc gagatgcgcc agatcggcgc gatgctgatc agcttgacgc tcaagacgtt
     2641 cgatcgcgtc cgcgacttcc aatcgcttgt cttgcaaagt cgaaataatc
//
LOCUS       DATMGX010000634         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_160661, whole genome shotgun sequence.
ACCESSION   DATMGX010000634 DATMGX010000000
VERSION     DATMGX010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160661"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1636)
                     /gene="dnaA"
                     /locus_tag="VNR65_10375"
     CDS             complement(167..1636)
                     /gene="dnaA"
                     /locus_tag="VNR65_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWJ19119.1"
                     /translation="MEALDLSTSIEHKWRRIQSRLRAELGEDVFTSWFGRVELEGYDG
                     RVVQVSVPTKFLRNWLQSHYSEKLIACCGSELGGVNCLEFRVRQPHLAQVEKLKPAVP
                     ATDMTAPRTQGSPATVTPLRALNMPARDGRTGLDGFEGSPLDPRYTFHSFVVGAPNRL
                     AHAAALQVAENGPDQPLKYNPLYIHARVGMGKTHVLHAIAWEMKQRNPSAKVLYLTAE
                     RFMYSFAEALQTRDAPAFKEKLRTIDLLLIDDMEFLQGRTFQQEFCHTLNSLIDGGKQ
                     VVVAADCAPLLLESFDARMRSRLSGGLVAELGALDYELRLKILHRRAGEKAAASIGFE
                     VPTNVLEYLATQLTENGRELEGAITRLHATYQLTGERISIDAAEGTIGDLIRSKEPRR
                     VKIDDILRIVAKHYGVQRGDLLSSRRNQSIVRPRQIGMYLAKTLTARSLPEIGRRFGG
                     RDHTTVLHAIRKIESLKQNDGTLKDELESLSRMLKDGTI"
BASE COUNT          593 a          750 c          742 g          570 t
ORIGIN      
        1 actatccccg tgatatgccc gagagcacgg agaaacgcat ttcggtcaac tacaaagcgc
       61 atattgacaa ctaccccgct tgggcggtgt tttgatttgg aacgccagtt tcacggttgg
      121 aagacgtctg tcaagtccag ccgctttaag agtgcatccc cgccagtcag atcgtgccgt
      181 ctttcagcat gcgcgagagc gactcgagtt cgtctttcag cgtgccgtcg ttctgcttca
      241 gcgactcgat tttgcggatg gcgtggagca cggtggtatg gtctcgcccg ccgaagcgcc
      301 tcccgatttc cggcaaagaa cgcgcggtca gggtcttcgc aagatacatt ccgatctgcc
      361 ggggccgaac gatggattga ttccggcggc tcgacaggag atcgccccgc tggacgccgt
      421 aatgcttggc gacaatgcga aggatgtcgt cgatcttaac gcgccttggc tccttcgacc
      481 ggatcaaatc gccgattgtg ccttcagcag cgtcgatcga tatgcgctcg cccgtcaatt
      541 ggtaggtcgc atgcaagcgc gtgatggcgc cttccagctc acggccgttc tcggtcagtt
      601 gggtcgcgag atattcgagc acattggtcg gaacctcgaa gccgatcgaa gctgcggctt
      661 tttcgccagc gcgtctgtga agaatcttca agcgcagctc gtagtcgagg gctcccaatt
      721 cggcgaccaa tccgcccgac aggcgagacc gcatccttgc gtcgaagctc tcgagaagaa
      781 gaggagcgca atcggcagcc acgaccacct gcttgccgcc gtcgatcaag gaattcagcg
      841 tatggcaaaa ctcctgctgg aacgtgcggc cttgcaggaa ctccatgtca tcgataagca
      901 ggagatcgat ggtccgaagc ttctctttga aggccggagc atcccgggtt tggagcgcct
      961 ccgcgaagct gtacatgaac cgctcggccg taagatacag caccttcgca ctcggattgc
     1021 gctgcttcat ctcccacgca atcgcatgca gaacgtgcgt tttgcccatg ccaacgcggg
     1081 cgtgaatgta aagcgggttg tatttcagag gctgatccgg cccgttctcg gcaacctgca
     1141 atgcagctgc gtgagccagc cggttcggcg cgccgacaac aaaggaatga aacgtatagc
     1201 gcggatcgag gggggatcct tcaaagccat caagacccgt gcgcccatcg cgagcgggca
     1261 tgttgagggc gcgaaggggc gtaaccgttg ccggcgaacc ttgggtccgg ggcgcagtca
     1321 tatcggttgc cggcacagca ggcttcagct tctcaacctg cgcaaggtgc ggctggcgaa
     1381 cccgaaattc caggcaattc acgccgccaa gttcgctgcc gcagcaggca ataagctttt
     1441 cggaatagtg agattgcagc cagttgcgca ggaatttggt cggaaccgac acctgcacca
     1501 ctcgaccgtc gtagccttcc agttcaacgc gtccaaacca gctcgtgaat acgtcttcgc
     1561 caagttccgc gcggaggcgg ctctgaatac gccgccattt atgctcaatc gaagtcgaga
     1621 gatccaacgc ttccatttca aaattccttc gccgccctat gggcatcgcg ttttcatcgg
     1681 aaaaagcttt tattctgcgg aagatattcc gggtctacgc tgccaaagcg agccccggtg
     1741 cctgattctc cgctttattg cgcggagcct cactggcttc cggcaaaaca gaatccagta
     1801 gcgacccagc ggaaggctgg ccagctgcct gcgtaaggac gctcaagctg gaaggacgct
     1861 cagtctctga ggaggaatgg cttgtggaag acggcgaaaa aacggcgtca gcaagatccg
     1921 atgctaaatt ctgaaattct cttgatgcaa gatacgtctt ccatgggatg gaagtatgag
     1981 ccgatctggg agctttacgc cgcgaccgcg atccggctcg agacgttgca tgatgggctt
     2041 gatcgactaa aaccggccgc atctgaagcg ttactggtaa cttccaagtg taagaacatg
     2101 tggagtgcgg caatgccgct atgcaattac ttcgcattca tttcctcctg cccaatgcct
     2161 tgcggacaga gaggtgagcc gtaaatagca cccggcgcgc accatccatc caatgcctga
     2221 tcatggacaa acacacactc gcgcacaggg gccaccccac acaacgagtt ctggtacgca
     2281 ttacaaggtt acaaattcag gagggaaaac tcccccgttc ggaagtctca ccatacaaag
     2341 ggcgctgaac ggacgcaaga gtggacacat gtcgcgaaaa tcgcccgatt ttcgtaatct
     2401 ttcgggatcg tcggcatgct tgcgatccct ttgtagggcg taaggctctg gaggaattaa
     2461 cgatttatta attacctacc ggagagttct acttagaatt gcgtgatcga cttgcgctcg
     2521 gaaaaagtgt caattcgacg ctggtccgcg cggttgtttt cagacatgcc aaaaggccca
     2581 gagatttgcc ttccctaggg ccttgattca acaacaattt tcaacttctt ggtctggcta
     2641 catcagaggg tggac
//
LOCUS       DATMGX010000635         3674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_44038, whole genome shotgun sequence.
ACCESSION   DATMGX010000635 DATMGX010000000
VERSION     DATMGX010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44038"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VNR65_10380"
     CDS             complement(<1..1589)
                     /locus_tag="VNR65_10380"
                     /inference="COORDINATES: protein motif:HMM:NF009379.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWJ19120.1"
                     /translation="MSGNGNAEGRSPRCVALVGPHPSGKTSLFEAILLRTGAAHEAAD
                     ARKSVGDTSAESKSHGMSVEMNVANFDFLGDKFTLLDCPGSVEFQYDGMGAIAACDAA
                     IVVCEADEKKLHALQIVLKSLETAGVPHFLFINKIDKVGIDKVGQDIGEVLTMMQPAS
                     RLPLVLRQIPIWEEGVVSGYVDLALERAFVYREHAPSEMIAIPSDLAKFEADQRFSML
                     EKLADYDDELMEQLLSDVTPPRDKIFQDLVTEFKQGQIVPVFFGSAQEGHGVGRLLKA
                     LRHDVPFAQETAKRLGLGSAKSAAYIMKTHHTSHAGKLSLARVLAGQFADGGSVYAPD
                     GEERISGVFSVVGTDVKKRGPGTVGETVALGKLESFPTGTTLTGEKSAPLSIKTSVPP
                     KPVYGLAIAAGERKDEIKLTAALNKLIDEDPSLVLIQDRDSNQIVLWGQGEMHLRVAL
                     ERLNRRFGIKAETKKRDIGYKETIRKSVEIRGRHKKQSGGHGQFGDVVVEIKPQPRGE
                     GFAFSEEITGGAVPRNYFPAV"
     gene            complement(1838..3439)
                     /locus_tag="VNR65_10385"
     CDS             complement(1838..3439)
                     /locus_tag="VNR65_10385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWJ19121.1"
                     /translation="MDALNQVNIPAQSKFTLKSLDCKSKSPAPAKTILAFIVACALAK
                     LFAAWVNGFTGDEAYTVVISRSFQLSYFDHPPLHQWIVHSFGALFGEGWWLCIPFLMM
                     AEAINLPLYGLTYRLFGRDAALWALFAFNATAYFAVWPDGLILPDVPLFLFLTIGVWA
                     IAEILFGPPRKRGAAWCLWLAAGAAFGLAGLSKYAAIFVPIGLFGFLAFSPRHRHWFQ
                     RPQPYLAAALALVIFSPALMWNYQNNWVSFAFQSGRAAPGASLSASAFATFGGALGAQ
                     VALLSPWVGVPLVFAVAHALRTAGAASASRFLLWLVAIPLLLFLSMPFMGKTAIPHWF
                     NSAWLFAFPLLGHSLSERSAKWLRPWAEASVALTAVCFALFVSYVTVGPFWRTASAKA
                     LKQDPTQWSYDWRGLKESPAWRGFSTETHAFVVVDNWRVGGKIGEAFGPDVPVCAFSQ
                     DPREFAFVCRENALLGQDALIVIPKERSQEALAALAPYFQRLDPSEEIAVGRGGRSER
                     LVTLTHAHVLLRPYPLPFGDMHQKL"
BASE COUNT          815 a         1045 c         1105 g          709 t
ORIGIN      
        1 tctacggcgg ggaaatagtt gcgcggcaca gcaccgccgg ttatctcctc ggagaaagcg
       61 aaaccctcgc cgcgtggttg cggcttgatc tccaccacga cgtcgccgaa ttggccatgg
      121 ccgccgctct gcttcttatg ccggccgcgg atttcgacgc tcttgcggat ggtttccttg
      181 tagccgatat cgcgcttttt cgtttcggcc ttgatgccaa agcggcgatt gagtcgctcc
      241 agcgcgacgc gcaggtgcat ctcaccctgg ccccagagaa cgatctggtt cgaatcgcga
      301 tcctgaatga gcaccagcga tgggtcttcg tcgatcagct tattgagcgc agccgtgagc
      361 ttgatctcgt ccttgcgctc cccggctgcg atggcgaggc cgtaaacagg cttcggcggg
      421 accgacgttt tgatggacag cggtgctgat ttttcacctg tgagcgtcgt gccggtcggg
      481 aagctctcaa gcttcccaag ggcgactgtt tcaccgacag taccagggcc gcgcttcttg
      541 acgtccgttc caacgacgga aaacacgccg gaaatcctct cttcgccgtc gggggcgtaa
      601 acagagccgc catccgcgaa ctgcccggca agcacacgcg ccagcgaaag tttgcccgcg
      661 tgcgacgtgt ggtgcgtctt catgatgtag gctgccgatt tcgcagaccc gaggccaagg
      721 cgcttggctg tctcttgcgc gaacggcacg tcatggcgaa gggccttcag cagccgtccg
      781 acgccgtgac cctcttgcgc cgagccgaag aagacaggga caatctgacc ttgcttaaac
      841 tcggtgacga ggtcctggaa gatcttgtcg cggggcggcg taacatcgct caggagttgc
      901 tccatcaact cgtcgtcgta gtcggcgagc ttctcgagca tggaaaaacg ctgatcggcc
      961 tcgaatttgg cgaggtcgga gggaatggcg atcatttcgc tcggcgcatg ctcgcgatag
     1021 acgaaagcgc gctcaagagc gaggtccaca tagccgctca cgacaccttc ttcccaaatc
     1081 gggatttgtc tgagcacaag cggaagccgg cttgcaggct gcatcatcgt caagacttcg
     1141 ccgatgtctt ggccaacctt gtcgatgccg accttgtcga ttttgttgat gaaaaggaaa
     1201 tgaggaacgc ctgcggtctc aaggcttttc agcacgatct ggagggcgtg cagttttttc
     1261 tcatcggcct cgcagaccac gatggcggca tcgcacgcag caatggcgcc catgccgtca
     1321 tactggaatt cgaccgagcc cggacagtcg agcagcgtga atttatcgcc aaggaagtcg
     1381 aaattggcga cgttcatctc gacgctcatg ccgtggctct tcgattccgc ggatgtgtcg
     1441 ccgaccgact ttcgcgcgtc cgcggcttcg tgtgccgcgc ccgtgcgaag taatatggct
     1501 tcgaaaaggc ttgtcttgcc gcttggatgg ggaccaacca atgcgacaca gcggggactt
     1561 ctgccctccg catttccatt tccgctcatg ctgttgttcc ttacgcggaa tatcttctat
     1621 tttgaagggt gaggcgcttc aaagtggagc tccgaatggg ccatggtggc aagagggctc
     1681 gtgctttgca agccccatca taagaaatcg acacatcgag aagggggctt attgacgcca
     1741 taggttccgg tagctacgga tcacgcgacg cgcttttggc ggagcctcgc ttccgcgctt
     1801 aacggcccac aatcgggggc tgaaaccgcc gagcaggcta cagtttctgg tgcatatccc
     1861 cgaagggtag gggatagggg cggagaagca catgcgcgtg cgtcagagtt acgaggcgct
     1921 cggagcgccc tccgcgtccc accgcaatct cctcgcttgg atcgagccgc tggaaatagg
     1981 gtgcgagcgc cgccaaagct tcctgggacc gttccttggg gatgacgatg agggcgtcct
     2041 gtcctaacag cgcattttcg cggcagacaa atgcgaactc gcgtgggtcc tgggaaaagg
     2101 cgcagaccgg cacatccggt ccgaaggctt cgcctatctt gccgccgacg cgccaattat
     2161 cgacgacaac gaaagcatgc gtttcggtgc taaagccgcg ccacgccgga gattctttga
     2221 gccctcgcca gtcatagctc cactgggtgg gatcttgctt caaggccttt gcgctcgccg
     2281 tccgccaaaa aggacccacg gtgacgtagc tcacaaataa agcaaaacac acggcagtca
     2341 gcgccacact cgcttcagcc caggggcgga gccattttgc gctcctctcg ctcagactat
     2401 ggccgagcag cgggaaggcg aacagccacg cggagttgaa ccagtgtggg attgcggtct
     2461 tccccatgaa cggcatcgag aggaagagta aaagcggaat cgcgacaagc caaagcagaa
     2521 aacggctggc gctggcggca cctgcggttc gcagggcatg cgcaacagca aagacgagcg
     2581 gcacgcccac ccatggcgag agcagcgcaa cttgcgcgcc gagcgccccg ccgaatgtcg
     2641 cgaaggcgga tgcgctcaaa gatgcgccgg gggctgcgcg gccggattgg aaggcgaatg
     2701 atacccagtt attctggtaa ttccacatca acgcggggga aaaaatcacg agcgcaagcg
     2761 ctgccgcgag ataaggctga ggacgctgga accagtgcct gtgccgaggc gagaaagcga
     2821 gaaatccgaa aaggccaatg ggcacaaaaa tcgccgcata tttcgaaagt cctgccaatc
     2881 cgaatgcggc gcctgccgcc agccagagac accaggctgc acctcgtttg cgtggcggcc
     2941 cgaacaggat ttccgcgatg gcccatacgc caatggtgag gaacaggaaa agcggcacat
     3001 ccggcaggat cagcccgtcg ggccacactg cgaaataggc cgtggcattg aatgcgaaca
     3061 aggcccacaa cgcagcatcc cggccaaaca gccggtaggt caggccgtat agaggaaggt
     3121 tgatcgcctc tgccatcata agaaagggaa tgcaaagcca ccagccttcg ccgaacagcg
     3181 cgccgaagct gtgtacgatc cattgatgca gcgggggatg gtcgaaataa gagagctgga
     3241 atgaccgcga gatgacgacg gtataggcct cgtctccagt aaatccgttt acccaggcgg
     3301 caaaaagctt ggcaagggcg caagcaacaa taaaggcgag aatggttttt gcgggggcag
     3361 gactcttgct cttacaatcg agggatttga gagtgaattt gctttgagcg ggaatattga
     3421 cctgattaag cgcatccatt tcaaacgcag ccaatgaaat ggttcaaatc acaggctgca
     3481 acgggtgcgt gcaaagaacg attcctcttt ggcattcagt gaagacgcag tcacacttct
     3541 taagaagaag catcagctac atccgtatga ttgcggcaaa gatgcggcat tgcatacagg
     3601 ctggaaaatt tcatgtcgcc acgtggggcc gctaacctag agtgcgtccg gaaaagtgcg
     3661 aagcggtttt ccga
//
LOCUS       DATMGX010000636         3149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_128798, whole genome shotgun sequence.
ACCESSION   DATMGX010000636 DATMGX010000000
VERSION     DATMGX010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128798"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VNR65_10390"
     CDS             complement(<1..1356)
                     /locus_tag="VNR65_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWJ19122.1"
                     /translation="MFDKTLGFVLRARRAEAPFWAERYVVAILLVLAAILLRLGFLQS
                     LGMSAAFITFYPAVILAALDGGFRAGMLASLLSAAAADYLWLEPAGTFSIAEPRDWVA
                     LGVFVASCTLISGVAEKLRQSNERLRWMKRTQRCELEGKIAERTALLRESEERLAAAL
                     RAGKLGVYDYNPRTNIIKWDSTVYRLFGVPEGESVTYETFEAGVHPEDIAAVREAVKQ
                     SLDPGGNHHYECEYRVISRGDGTMPWVFADGDVTFDADGPCRLVGTVQDITGRKQAEA
                     TLRESEERIRIAQQAARIGTFEWNIETGVNTWTLELEALYGLPPGAFGRTQPAFESLV
                     HPEDRAGTIRLVEQAMASGELTTGEWRVQWPDGSIHWLSGRWQVFKDSSGKPLRMTGI
                     NLDITDRKQAEEALRQSEMRYRMLHESLRDAFVQVSMDGRIVECNDLYCQLVGYSREE
                     VS"
     gene            1936..2970
                     /locus_tag="VNR65_10395"
     CDS             1936..2970
                     /locus_tag="VNR65_10395"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWJ19123.1"
                     /translation="MSYRASRLALCRRAFLVRQAQVLAIAAAAWFCVAPLHAQTTRTL
                     KIGYILSKDSQLGAGATAFAEEVAKRSGGRLAVEQYPNSALGGEVEMLKALQLGTLDL
                     AFITGAPLPTVVPEVGAFNIPFIFRDLDHAHAVLDGPIGQDYLKKFESKDLVALAWGE
                     NGMRHLTNSKREIHTPADLKGLKLRLPQSEVMLIGFKALGADPALLPFPQVFGALQSG
                     QFDGQENPIATIMSAKFAQVQKYLTLSGHVYDPAVFLASPDMFNELPDEDKKIFIEAA
                     RAGAQASRKYAAEAQEKGVAALRQAGMTVTTDVDRTLFSKAMASAMPDYEKRFGAEVI
                     QRMRQTGAGL"
BASE COUNT          655 a          948 c          859 g          687 t
ORIGIN      
        1 actcacttct tcacgcgagt atcccaccaa ctggcaataa agatcgttgc attcgacgat
       61 tcgcccgtcc atcgaaacct gcacaaaagc gtcacgcagg ctttcgtgaa gcatccggta
      121 ccgcatctcg gattggcgca atgcttcctc tgcctgcttg cgatccgtaa tgtccaggtt
      181 tatgcccgtc atccgcagcg gcttgccaga gctgtctttg aaaacctgcc agcggccaga
      241 gagccagtgg atgcttccat ccggccactg cacccgccat tcgcccgttg tcagctcgcc
      301 cgatgccatg gcctgttcaa cgagccggat tgttccggcg cggtcctctg gatgaaccag
      361 actctcgaac gcgggctgcg ttcgcccaaa cgcaccgggc ggcaagccgt acagcgcttc
      421 gagttccagc gtccaggtat tgaccccggt ttcgatattc cattcaaacg tgcctatgcg
      481 agccgcctgc tgcgcaatac ggatccgctc ttcgctttcg cggagcgtcg cctcagcctg
      541 cttgcggcct gtgatgtcct gcactgtgcc cacaaggcgg catggcccgt cagcgtcgaa
      601 cgtcacgtcg ccatcggcga agacccaagg catcgttcca tcgccgcggc tgataacgcg
      661 atattcgcat tcatagtgat ggttgccgcc aggatcgaga gactgcttta ccgcctctct
      721 gacagcggca atgtcctcgg gatgcacgcc tgcctcgaac gtctcgtagg tgacggactc
      781 gccctccggc acgccgaaca ggcgatagac agtggagtcc cattttatga tattcgtgcg
      841 cggattgtag tcatacacgc cgagctttcc agcacgaagc gccgcagcga gtcgctcctc
      901 gctttcacgg agcagcgccg tgcgctcggc aatcttgcct tcgagttcgc agcgctgggt
      961 tcttttcatc cacctcagcc gctcattgct ctggcgcagt ttttcggcaa ccccggatat
     1021 gagagtgcaa ctggcgacaa atactcccag agccacccaa tctctcggtt cagctatgct
     1081 gaaggtccca gccggctcca gccacaagta atcggcagct gccgccgaga gcaggctggc
     1141 caacattcca gcccgaaagc cgccatcgag cgccgccagt atcacagcgg gatagaaagt
     1201 tatgaaggcg gcgctcatcc cgagactttg caggaaccct agccgcagga ggatggcagc
     1261 gaggacgagg aggatggcaa cgacgtatcg ttcggcccag aatggcgctt cagcccttct
     1321 agccctcaag acaaaaccca aggtcttatc gaacatagtt attcggttcg accgtgctgg
     1381 aggggtgata catgttttgc cgccgcgatt cctcagtcga gtactttcta gcgctgcatt
     1441 tagctcaaac cgttgctccg acagttacat ccgagagata aactctgaat ccggaactgc
     1501 tcttcggccg atccgttatc ggacgaaggc aaacggccat gccagagttc agcttcaata
     1561 attgataagc agccatcgag caagagcttg cgccgaaacg gcggccactt gcggtcacga
     1621 tttcgagcgg ccagctcggg gtcgctgttt actcaccgga acgctccaag caatccgtgg
     1681 aatgcgaacg gttcggtagc ggaagaggat tttgttaaat aacaaccgtc agccgatcgg
     1741 gtttgttctg cgatgataca cgcactcgta cacgaaacta ccggtccttc cgtgccggaa
     1801 ctccggctta agcaaagtat aaatttaaat aaacaatcgt acggaaacgt atagttccca
     1861 ttttaaggca ggtgccgatg tattattaac tcacttctgt gaatgtacgg cgacagctat
     1921 tctttggagg ttccaatgtc ataccgtgct tcccggctcg cattgtgcag aagggctttt
     1981 ctagtccgcc aggcgcaagt cctcgcaatc gcggcggcgg cttggttttg cgtggcgccg
     2041 cttcatgcgc aaacgacccg cacgttgaaa atcggttata ttttatcgaa ggattcccag
     2101 ctcggggctg gcgccacggc gttcgccgaa gaggttgcca agcgcagcgg cggccggctc
     2161 gccgtggagc aatacccgaa ctccgccctc ggcggcgaag tcgaaatgct aaaggccctc
     2221 caactcggca cgctggatct ggcctttatt acaggtgcgc ccctgccgac tgtcgtgcca
     2281 gaggttggcg cattcaatat ccccttcatt ttcagggatc tcgaccatgc ccatgctgtg
     2341 ctcgatgggc cgatcgggca ggactatctc aagaagttcg agtcaaaaga cctcgtggcg
     2401 ctcgcctggg gcgaaaacgg catgcgccac cttaccaact ccaaacgcga aattcatacg
     2461 ccagcggact tgaaaggact gaaactccgt ttgccgcagt ccgaggtcat gcttattggc
     2521 ttcaaggcgc tgggcgccga tcccgccctt ctgccattcc cgcaagtatt cggcgcgctg
     2581 cagtcgggac agttcgacgg tcaggaaaat ccgatcgcaa ccatcatgtc ggcgaaattc
     2641 gctcaggtgc aaaaatacct gacgctttcg ggccacgtgt acgatccggc tgtcttcttg
     2701 gcctcgcctg acatgttcaa tgaattgccg gacgaggata agaagatctt tattgaagcc
     2761 gctcgcgccg gcgctcaggc ctcgcgcaaa tacgcggccg aagcccagga gaaaggtgtc
     2821 gccgcgctac gtcaggctgg aatgaccgta acgaccgatg tggaccggac gctgttttcc
     2881 aaggccatgg cttccgcgat gcctgactat gagaagcgtt tcggcgccga agtcatccag
     2941 cgcatgcgcc agaccggtgc cggcctatag aggcgatcct cggcaaggtt tcgagactct
     3001 ctgcgggaat gacggctgcg ctgttcttcc cgcgtttcac gctttgattt tgggactgtg
     3061 aacaatgaca tggacatcca tcagtcatcg gttgtcgatc aggctccaga tctttggcgg
     3121 gttcggactt ctcatcgcgg tctttcttg
//
LOCUS       DATMGX010000637         2059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_70018, whole genome shotgun sequence.
ACCESSION   DATMGX010000637 DATMGX010000000
VERSION     DATMGX010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70018"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VNR65_10400"
     CDS             <1..872
                     /locus_tag="VNR65_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWJ19124.1"
                     /translation="LGAIATLVFIKAILLTGLATLFGVPRGAALETALLLGPAGEFAF
                     VGIGLATKLGLLSGSLSSFVLAVTSLSMAFIPVLSMAGRRISLKLQERDRHLDPNLSA
                     VPRKRQGHAIVVGHGRVGQVLCSLLERHVYPFIASDRDPDLVTEHRRCGRDVYFGDAT
                     NPAFLKSCGIDHAKAVIVTATGCKDIDAIVKAARLARPDILIVSRARDAAHASHLYEI
                     GVTDAVPETVEASLQLSEASLVGLGLPVGLVIASIHEMRDVFRTELQNAARRSGRPIT
                     RAIRRKTLRDRFH"
     gene            1623..1748
                     /gene="ykgO"
                     /locus_tag="VNR65_10405"
     CDS             1623..1748
                     /gene="ykgO"
                     /locus_tag="VNR65_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HWJ19125.1"
                     /translation="MKVKNSIKTLRTRHRDNQVVRRRGRLYIINKKVRRFKARQG"
BASE COUNT          424 a          579 c          607 g          449 t
ORIGIN      
        1 tgctcggcgc gatcgcaacc ttggttttca tcaaggcgat tctgctcacg gggctcgcca
       61 cgctgttcgg agtgccgagg ggagcggcgc ttgagactgc gctcctgctt ggccctgcag
      121 gcgagttcgc ctttgtgggc atcggcctag cgaccaagct cgggttattg agcggtagcc
      181 tgtcgagctt tgtgcttgcg gtgacttcgc tatccatggc cttcattcct gttctcagca
      241 tggctggcag acgaatctcg ctgaagctgc aggaaagaga ccggcatctc gatccaaacc
      301 tttccgctgt cccgcgtaag cgtcaagggc atgccatcgt tgttggccac gggcgagtgg
      361 ggcaggtgct ttgctcgctc ctggagcggc acgtctatcc cttcattgca agcgacaggg
      421 accccgacct cgtcacggaa cacagacgct gcggccgaga cgtctacttt ggcgacgcga
      481 ccaatccggc tttcctcaag agctgcggga tcgaccacgc caaggcggtt atcgtaaccg
      541 ccacagggtg caaagacatc gatgcaatcg ttaaagccgc gcgcctggcg aggccggata
      601 ttctgatcgt gtcccgtgcg cgagacgcag ctcacgccag ccacctttat gaaataggcg
      661 ttacggatgc cgtgcccgaa accgtagaag cgagccttca gctttcagag gcttcgctcg
      721 ttgggcttgg tcttccggtg gggctggtga tcgcttcgat ccacgagatg agggacgtat
      781 tccggactga gttacagaac gcggcacgga gatccgggag gccgatcacg cgggcgatcc
      841 gaaggaagac actgcgcgac aggtttcatt gagccggacg atccgttcag cttcgagtgg
      901 ttgttatgcg aatcgccgtc ctttgcccat cgccgttctc ggcaaaaagc aaattactta
      961 tatgtttact tatagcctct ttacgaaaca aaaccaatct tagccgatcg ttaactctaa
     1021 cagcctgacg tgcgtgaaga tgccgaacga cggttcgtat cagtggccgg ccgctggccg
     1081 ggtggacgtg attgcattgg ctggcaggaa cgggttcctg acaatcgctg gcgctaatgc
     1141 attttccttc ttggacggaa tgcgtcggtg aaaggcagag ggtgaatcgc aaacactgct
     1201 gctgttggtc ctttggcttt gcaaagggat cagcgcttga acggggctgg aagggcctag
     1261 ctgagtgcac gccgcagggg cgttcgccgc actcgccgcc gtggtagcgg ctgccgagac
     1321 aatcacggct aaaaaaacgc gggacaaaat catggtacgg agcacgcaac gcatcgacga
     1381 cgctaacaga ctgatttccg gacaatagcg ctcctcgcgt ctgccgtcgc catcaatttg
     1441 atggggatca gtatggcttg cgatttttct ttagaacggg aaagggttgg aaacggtgaa
     1501 aaagcgtgat agcaattctg cctgacggcg cttcagacct gcgcgcttcg caaacctcaa
     1561 cttattggct tgcgcttcca ccggcccaac cggagactgc gtggaccgct tggaatattg
     1621 ctatgaaagt caagaactcg atcaagaccc ttcgcacgcg gcaccgcgat aaccaagtcg
     1681 tgcgccgccg gggcaggctt tatatcatca acaagaaggt gcggcgtttt aaggcgcggc
     1741 aaggctaggc ccagcatcat cttctaaacg gcgcgattcg caattagagc gaattcggcg
     1801 atcttcgagt cgccgaattc gctctaagct tttatcgtgt cgcgcttccg ggcggaaaac
     1861 cgcatacact tttcctggaa agcgctctag gtcgcgccgt tcgattcgcc gatcttgaag
     1921 acgcgcagca tgttggtcgt accgggtctc ttaaagggaa cgccggcgca aacaatcacg
     1981 cggtcgccat tggccgcgta gccttcggca cgaacgaccg cctccgcgcc tttcaccatt
     2041 tgcgttgtat tcgccggat
//
LOCUS       DATMGX010000638         3399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_132649, whole genome shotgun sequence.
ACCESSION   DATMGX010000638 DATMGX010000000
VERSION     DATMGX010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132649"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..1042
                     /locus_tag="VNR65_10410"
     CDS             452..1042
                     /locus_tag="VNR65_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19126.1"
                     /translation="MPSPYLNYALAALSFGLAVAFFRISDENSALTQAKIAVEMRLET
                     AEGEAKSFREKYATEEKAREFAEAAQTLAETSERAVRSQLAHETKAHQAAEKSLEEAE
                     NARHSVEAALSDALEQVRVLNAKLVEPSAAKEQSERKADASASDKGDAAAADAKEPAA
                     HIGDGSPAVSAVPAGAMAAQASMAGSRPWYSFLGLF"
     gene            1230..1754
                     /locus_tag="VNR65_10415"
     CDS             1230..1754
                     /locus_tag="VNR65_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HWJ19127.1"
                     /translation="MHFWSTVLAVVAATAIAVLAVFFIVGPERIWALFGPADLGPVTF
                     ETLNRRSSPNDSLACPQNICTAKSDVIPPIFALSAPELAKVFAKVISSEPSVEQVALD
                     DGGLTRRYVQRTKIMRFPDTIVVRFIDLGDGRSTIALYSRSQLGEGDFGVNRARIERW
                     LEKLSKEAPAVKTA"
     gene            complement(1768..2172)
                     /locus_tag="VNR65_10420"
     CDS             complement(1768..2172)
                     /locus_tag="VNR65_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19128.1"
                     /translation="MADTFHFELTSPEQTVVSADLSEAILPGQEGDLAAMPGRALMIM
                     KLRPGILTTRSKEGEQQYFLRGGFADVGPEQTVVLTEYAIPLAQLTGDILADEIAQAQ
                     QALDEAPEDGRRISAWDRLERLQTLRERLALK"
     gene            complement(2265..>3399)
                     /gene="atpD"
                     /locus_tag="VNR65_10425"
     CDS             complement(2265..>3399)
                     /gene="atpD"
                     /locus_tag="VNR65_10425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWJ19129.1"
                     /translation="GPIKSNQRRPIHAAAPAFVDQSTEVEVLVTGIKVVDLLAPYSKG
                     GKVGLFGGAGVGKTVLIQELINNIAKAHGGYSVFAGVGERTREGNDLYWEMIESHVNV
                     DPHEHNGSSKGSKCALIFGQMNEPPGARARVGLTGLTVAEYFRDQGQDVLFFVDNIFR
                     FTQAGSEVSALLGRIPSAVGYQPTLATDMGALQERITSTHKGSITSVQAIYVPADDLT
                     DPAPATSFAHLDATTVLSRSIAEKGIYPAVDPLDSTSRALEPRIVGEEHYQVARRVQS
                     ILQRYKALQDIIAILGMDELSEEDKRIVARARKIERFLSQPFHVAEVFTGKSGVLVDV
                     KDTVKGFKGLCDGDYDHLPEQAFYMVGTIDEAIKKAEELAAAA"
BASE COUNT          744 a          962 c          983 g          710 t
ORIGIN      
        1 tacgtctttc cgattgtttt cgacgatgga ccggagcaca tttcgaccga tacggccgaa
       61 gccgttgatt gctaccctca cagtcatgtt gctctatccg ctggaaacga ggttcgcgcc
      121 ctacgcattt ttgcgcggta accttgaccg catacgcccc gaaacgcaag agcaagcttt
      181 ctgccggagg ctgaattggc aattcattgc gcgttgcttt gcagacaagg tgtgcaggag
      241 ggtatcgagt aacagatcgc ctattccaca cagtcagcag gtaattaaga agccgctaat
      301 tgcttaatcc tcccagcact gttgccatga cagcacaagg ttacgctttt gcagataaga
      361 cttgataaaa gtcagacgtt aacatttaag tcatccgaat aatgaagaat caatcctgcc
      421 aaacgccgtt aacacttcct aaggaaatac catgcctagc ccatatctta actatgcgct
      481 cgctgctctg agcttcggcc ttgcagtcgc attttttcgc atcagcgacg aaaatagcgc
      541 gctcacacag gcaaagatcg ccgtcgaaat gaggctcgaa acagcggaag gggaagcgaa
      601 gtccttccgt gagaagtatg caaccgaaga gaaagcacgc gagttcgctg aagccgctca
      661 gaccttggcg gaaacatccg agcgcgcggt tcgctcccaa cttgcccatg agacgaaagc
      721 ccatcaggcc gcagaaaaga gcctggagga ggcggagaat gcaagacatt ctgtggaagc
      781 cgcgctttca gacgccctgg aacaggttcg cgtgttgaat gcgaagctgg tagaacccag
      841 cgcagcgaaa gagcagtccg agcggaaggc tgacgctagc gccagtgaca agggcgacgc
      901 cgctgcggcc gacgctaaag agccggctgc gcatatcgga gatgggtcac ccgcagtaag
      961 tgctgttccc gcgggagcga tggccgccca ggcatccatg gccggcagca gaccctggta
     1021 ttctttcctc gggcttttct aagaacccgc cgaccgtcag tgaagtgtgt cgcaagcgtt
     1081 agaatgatat atgtgcgagg cgtcggcaga cgcgtaaagg agccttttcg ggctccttct
     1141 tgtttctttg atcttatgaa agcggatgtc ttctttccga aatagtgtta gaggagcggt
     1201 ttcgatagca ccgcagaccg gggtttccga tgcatttctg gtcgacagtt cttgccgttg
     1261 tggctgcaac cgcgattgca gtcttagccg tattcttcat cgtgggaccg gagcgcattt
     1321 gggccttgtt cggacctgcg gatctcggcc ccgtcacttt tgagacgctc aaccggcggt
     1381 catcaccgaa cgattcgctt gcctgccccc aaaacatttg caccgcgaaa agcgatgtga
     1441 tcccgcccat tttcgcgctg agtgctcctg agttggcaaa ggtcttcgcg aaggtcatct
     1501 cctcggagcc aagtgtggaa caggttgctt tggacgatgg cggcctcacc cggcgttacg
     1561 ttcagcgcac aaaaatcatg cgctttccag acacgatcgt cgtgcgattt atcgatctcg
     1621 gggatggacg gtctacaatc gccctttata gccgctcaca gcttggggaa ggcgactttg
     1681 gcgtgaaccg cgctcgcatc gagcgctggc tggagaaact gtcgaaggaa gctccggctg
     1741 tgaaaacggc ctgagcaggc aagcgagtta cttgagagcc aagcgctctc ttaatgtttg
     1801 gagacgctca aggcgatccc acgccgaaat acggcgtccg tcttctggcg cctcatcgag
     1861 ggcttgctga gcctgagcga tttcatcagc gaggatgtcg ccggtcaatt gagcgagagg
     1921 aatagcgtac tcggtgagga ccacggtctg ctcggggcca acatcagcaa aacctccgcg
     1981 caggaaatat tgctgctcgc cttcctttga gcgcgtggtc agaatgccgg ggcgcagctt
     2041 catgatcata agcgcacgcc cgggcatggc cgcaaggtca ccttcctggc cagggagtat
     2101 agcctcggaa agatcggcgg agacaaccgt ctgctccggc gaagtaagct caaagtgaaa
     2161 tgtgtcggcc atataagtcc gctctcggct tagtcatgtg ggaaaccgat atccggaccc
     2221 gtggcgacgg ctacacgatc gccacgagac cgaaaaggtg aaggttacgc cgctgcggca
     2281 agctcctcag ccttcttgat tgcctcatcg atcgtgccga ccatgtagaa ggcctgctcc
     2341 gggagatggt catagtcacc atcgcacaat cccttgaagc ctttcacggt gtccttgacg
     2401 tccacgagaa cgccagactt gccagtgaac acttccgcga cgtggaaggg ctgcgaaagg
     2461 aaacgctcga tctttcgggc gcgcgcgacg atgcgcttgt cctcttcgga gagctcgtcc
     2521 atgcccagga tcgcgatgat gtcctgaagc gccttatagc gttgcagaat cgattgcacc
     2581 ctgcgggcta cctgatagtg ctcctcgccg acgattcggg gctcaagcgc gcggctcgtc
     2641 gagtcgagcg ggtccaccgc aggatagatg cccttttcag cgatggaacg cgacagcacc
     2701 gtcgtcgcat ccaagtgcgc gaaggacgtg gccggggcag ggtcagtcaa gtcgtcggca
     2761 ggcacgtaga ttgcctgcac cgacgtaatc gaacccttgt gtgtggaggt gatgcgctct
     2821 tgcaaggcgc ccatgtcggt ggcgagcgtc ggctggtagc ccaccgctga aggaatacgg
     2881 ccgagcaaag ccgacacttc ggagcctgcc tgtgtgaagc ggaagatgtt gtccacgaag
     2941 aacaacacgt cttgcccctg atcgcggaaa tactctgcga cggtcaagcc ggtaaggcca
     3001 acacgcgcgc gtgctccggg cggctcgttc atctggccaa agatcagcgc gcatttcgaa
     3061 cccttcgacg agccattgtg ctcgtgggga tcgacgttca cgtgagactc gatcatttcc
     3121 cagtaaaggt cgttgccttc acgcgtccgc tcgcccacgc cagcgaatac cgaatagcca
     3181 ccgtgggctt tcgcgatgtt gttgatcagt tcctggatga gcacagtttt gcccacgccg
     3241 gcgccgccga acaggccgac cttgccgccc ttggaataag gcgcgagcaa atccacaacc
     3301 ttgatgcccg taaccagcac ttcgacctcg gtcgattgat cgacaaaggc aggcgcggct
     3361 gcatgaatgg gtcggcgctg gttcgacttg attggccct
//
LOCUS       DATMGX010000639         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_47725, whole genome shotgun sequence.
ACCESSION   DATMGX010000639 DATMGX010000000
VERSION     DATMGX010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47725"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VNR65_10430"
     CDS             <1..245
                     /locus_tag="VNR65_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWJ19130.1"
                     /translation="LGLSVTEGAKVLGVTRQALNNIVNGKSGISPEMAIRLTKAFGST
                     PETWLGIQIAYDLAAARKHENKIKVRRQRVEELRAG"
     gene            332..1570
                     /locus_tag="VNR65_10435"
     CDS             332..1570
                     /locus_tag="VNR65_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ19131.1"
                     /translation="MTTIPWQGRHPSHKARLLAWLLLLLPIAVIAIYPLARDYLQAAS
                     LLQRISDPNATGWLTNYEVHPVDVRDATFNFRGKPVPVHVYTPRGIGFAPGIVIVHGM
                     HRLGIDDPRLVNSARAVASSGFFVMTPLVSGIADYRVERESADLIGAAAHSFAQELNV
                     PKVGILAISFSGGLSLLAASNPQFSPSIAWVASVGGYYDLAHVLRFFATGEAVRPDGS
                     IEPMQPHEYGPLIVIYDEPQDFFAPEDVQTAHEALRLLLSEDGKASEALTKTMTPDDQ
                     EIMQRIYRKQRESFTPAILAEIDKRREKLAAASPAGHLQSLHVPVLLLHGSDDVIIPP
                     TEMLWLQRDIPKQYLLDALISPAIGHVNFDAKVTLHDRLALVHWMSLMIHEARTAGDG
                     QDSMHLPAGLWLASVATTVH"
     gene            1578..>2267
                     /locus_tag="VNR65_10440"
     CDS             1578..>2267
                     /locus_tag="VNR65_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HWJ19132.1"
                     /translation="MVSLEQIQLAQKRLRGVAARTPLIAYFPPARRENSAADAKSADE
                     STRGQLWLKAESLQPIGSFKLRGAYNKIASFSEEERQRGVISYSSGNHAQGVAYAARA
                     LGVKAVIVMPRNAPKIKMDSTAALGAEIVTVGPASAKRLQKAEELARQHGYMIVPPYN
                     DEQIIAGQGTVGIEILEDCPDVDLVLAPVGGGGLISGVSAAVKLSGSKAKVIGVEPEL
                     ANDAQQSFRMGA"
BASE COUNT          448 a          687 c          687 g          445 t
ORIGIN      
        1 ctttgggact gtcagtgacg gaaggggcaa aggtgctcgg ggtcacccgg caggcgctca
       61 acaatattgt taacggcaag tccggaatca gcccagagat ggcgattcga ctgaccaagg
      121 cctttggcag tacgccggaa acctggctgg gaatccaaat tgcgtatgat cttgccgcgg
      181 cgcgtaagca cgagaacaaa attaaggtgc gccgccaacg cgtagaagaa ctacgcgcgg
      241 gctagtcgta ttagatggtg caaaatttct ggctcagacg tgcagcagag caccgactca
      301 aaatggcttc gaacgtcccc actctctacc catgaccacc atcccctggc aaggccgcca
      361 tccatcgcac aaggcacgcc tcctcgcctg gctgctgctg ttgcttccca tcgccgtgat
      421 cgccatctat ccgctcgcgc gagactatct gcaggctgct tctctgctgc agcgcatttc
      481 cgatccgaat gccaccggat ggctgaccaa ctatgaagtg catccggttg acgtgcgcga
      541 tgccaccttc aacttccgag gcaagccggt tccggtgcat gtgtacacgc cgcgaggaat
      601 cggcttcgcg cccggcatcg tcattgtcca tggcatgcac cggctgggga tcgacgatcc
      661 tcggctggta aattccgcgc gcgccgtggc gtcgagcggg ttctttgtga tgacgccgtt
      721 ggtctctggc atcgccgact atcgcgtgga acgcgagtcc gccgacctca tcggcgcggc
      781 tgcgcacagc ttcgcgcagg aactcaacgt gccgaaagtg ggcatcctgg cgatcagctt
      841 ctccggaggc ttgtcgttgc tggccgccag caatcctcaa ttctcgccgt cgatcgcatg
      901 ggtggcttcg gtgggcggat actacgatct cgcccatgtt ctgcgcttct ttgcgacggg
      961 cgaggcggtt cgtcccgatg gatcaatcga gcctatgcag ccgcatgagt acggtccgct
     1021 gatcgtgatt tatgacgagc cgcaggactt cttcgctccc gaggacgtcc agacggcaca
     1081 cgaggccctc aggctgctgc tctcggaaga cggaaaagct tccgaagcgc tgaccaagac
     1141 catgacgcct gacgatcagg agatcatgca gcgcatttat cgcaagcagc gtgagagctt
     1201 cacgcctgcg attctggctg aaatcgacaa gcggcgggag aagttggctg ccgcttctcc
     1261 ggccggtcac ctccagtccc tgcatgtccc tgtcctgttg ttgcacggct ccgacgatgt
     1321 gatcatcccg ccgactgaaa tgttgtggct gcaacgtgac atcccgaaac agtatttgct
     1381 ggatgcacta atcagcccgg cgattggtca cgttaatttt gacgccaagg ttacgttgca
     1441 cgatcgtctg gcacttgtac actggatgtc attgatgatc cacgaggcgc ggaccgccgg
     1501 cgacggccag gactcgatgc acctgcccgc cgggctgtgg cttgcgtccg tggcgacaac
     1561 ggtgcactga cccagccatg gtgagtctcg aacagatcca actcgcgcag aagagattgc
     1621 gcggcgtagc ggcccgcacg ccgctcattg cctactttcc gcccgcccgc agggagaact
     1681 ctgctgccga tgcgaaatcc gccgacgaat ctacgcgcgg gcagctgtgg ctgaaagccg
     1741 agagtcttca gcctatcggc tcgttcaagc tgcgcggggc ttataacaaa atcgcttcgt
     1801 tcagcgaaga agagcgccag cgcggagtga tctcctattc cagcggcaat cacgcgcagg
     1861 gagtggccta tgcggcacgc gccctgggag tgaaggcggt cattgtaatg ccgcgcaacg
     1921 cgcccaagat caagatggac agcaccgccg cgctgggagc ggagattgtc accgtcggtc
     1981 cggccagcgc caagcgtttg cagaaggccg aagaactggc gcggcaacat ggctacatga
     2041 tcgtgcctcc gtacaatgat gagcagatca tcgctggaca gggtaccgtc gggatcgaga
     2101 tcctggaaga ctgccccgac gttgacctcg tgctggcgcc ggtcggcggc ggcgggctga
     2161 ttagcggggt ttccgctgcc gtgaagctga gcggctccaa agcgaaagtt attggcgtcg
     2221 agcctgaact cgccaatgat gcgcagcaaa gcttccgcat gggcgcg
//
LOCUS       DATMGX010000640         2256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_295869, whole genome shotgun sequence.
ACCESSION   DATMGX010000640 DATMGX010000000
VERSION     DATMGX010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295869"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VNR65_10445"
     CDS             complement(<1..186)
                     /locus_tag="VNR65_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19133.1"
                     /translation="MLPRTAMGKWIASEDLRGYPGNTRSSEDCWMAGASPAMTRKQIR
                     HERSLTPPRNPASARHPL"
     gene            681..1265
                     /locus_tag="VNR65_10450"
     CDS             681..1265
                     /locus_tag="VNR65_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWJ19134.1"
                     /translation="MIGKLKHCALGTALAAVLPLAPAAAQEFPIDTAGISKAVISETN
                     AYRASKNLPAVQENAALQAAATAYAKFLAEKEAAGHTADGQTPAKRVKAQGYQYCHVA
                     ENMWGGWRRPDPMSDDQAASKAMDDWKKSPGHNANLLGKQAVDIGIGAAAWRQSGNRV
                     VFRIVQVFGKDCSGKSGPTASFGDVVNAVGKQLR"
     gene            complement(1288..>2256)
                     /locus_tag="VNR65_10455"
     CDS             complement(1288..>2256)
                     /locus_tag="VNR65_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HWJ19135.1"
                     /translation="ERTSSFSQNGKGLQSGDAPLVVYASDQGHSSIEKAALLAGFGKA
                     HLRLLSSDENHALRLDLLEAAIAKDLEAGLRPCALVACIGTTGTTAVDPLPQMADIAA
                     RYGMWLHVDTAMVGTAMVLPECRVHFAGVERADSLVLNPHKWMGVGFDFSAYYVRDPE
                     HLIRVMSTNPSYLQTKQDGEVKNYRDWHIQLGRRFRALKLWFYLMDVGVEGLQARLRR
                     DLANAQWFKDQVDAGADWERVAPVPFQTICIRHVPGALAGDEDALKAHNLEIARRVNE
                     GGGAYLTPSILKGEQILRVSIGAEGTERAEVEALWTLLNEAAAASP"
BASE COUNT          471 a          673 c          678 g          434 t
ORIGIN      
        1 gagcggatgc cgggcggaag cgggattgcg gggaggcgtg aggcttcgtt catggcgaat
       61 ttgtttcctc gtcatggccg ggcttgcccc ggccatccag cagtcctcgg aactgcgcgt
      121 gtttccagga tagccgcgca agtcctcgga cgcgatccat ttgcccatcg cagtccttgg
      181 caacatggga aagtttcgcg cgtgaagttt cggttaacag acctgattaa aaaagcgttt
      241 cgccctcacg cgcgaggagg tttggatgcc cttcccgagc aacgcgattt tccggaaata
      301 ttaaatgaac gatatttatt gccataaaat tgcaaatttg cccatagtac tactccataa
      361 attacttgta tttctactta tggccgtttc tacgaaacaa aaccaatttt aaccagctgt
      421 caactctcgt aggatcggag ggagcgttcc agcgaagcgg caaatcccgg acgaggcatt
      481 ttccgccccc tcgccctcgg tgccttaaat gacctcctga cagtatggag ctgcctcaac
      541 gggcgagcca aatggcgaca tcccatcggc agcgcaaatc atgtttgtcc acagccggaa
      601 gacgattttt ggagatatgt tatttgcgcc aaagaatccg gctagacgct ctaacttcgt
      661 ttgaaagctg gatgagaaga atgatcggca aactcaagca ctgtgccctg ggtaccgcct
      721 tagcggcagt tctcccattg gccccggctg ccgcacagga atttccgatc gacacggcag
      781 gcatctcgaa agcggtgata tccgaaacca acgcctacag ggcgtccaag aatctgccgg
      841 ccgtgcagga gaatgcggcg ttgcaagccg ccgcgacggc ttacgcgaag tttctcgccg
      901 agaaagaagc cgcaggccat accgccgatg gccagacgcc tgcgaagcgg gtgaaagcgc
      961 agggctacca gtattgtcac gtcgcggaaa atatgtgggg cgggtggcgc aggcccgatc
     1021 ccatgagcga cgatcaggca gccagcaagg cgatggacga ctggaagaag tcgccggggc
     1081 ataacgccaa tcttctcggg aaacaagccg tggacatcgg tatcggggcg gctgcctgga
     1141 gacaaagcgg caaccgcgtt gtgttccgca tcgtgcaggt tttcgggaaa gactgctcag
     1201 ggaaaagcgg gcctacagcg tccttcggag acgtggtcaa cgccgtcggc aaacagctca
     1261 ggtaagccag agccctgagc cgaggattta tggcgatgcg gccgccgcct cgttcagcag
     1321 cgtccataag gcctccacct cggctcgctc cgtgccctcg gcgccgatgc tcacgcggag
     1381 aatctgctct cccttcagga tggaaggggt gaggtaggcc ccgcctccct cgttgactcg
     1441 ccgggcgatt tccagattgt gggccttgag cgcgtcctcg tcccctgcaa gcgcgccggg
     1501 aacatggcga atgcagatag tctgaaaggg taccggggca acccgctccc aatctgcgcc
     1561 ggcatccacc tgatccttga accattgcgc attggcaaga tcccggcgga ggcgggcctg
     1621 aagcccttcc acgcccacat ccatgaggta gaaccaaagc ttcagggctc tgaagcgccg
     1681 acccagctgg atgtgccagt cccggtaatt cttgacctcg ccgtcctgct tcgtctgcag
     1741 gtagctcgga ttggtgctca tgacgcggat caggtgctcc ggatcgcgca catagtaggc
     1801 gctgaagtcg aaccccacgc ccatccactt gtgcgggttc aaaacaaggg agtcggcgcg
     1861 ctcgacgccc gcgaagtgca cgcggcactc gggtaggacc atcgccgtcc cgaccatggc
     1921 ggtgtccacg tggagccaca tgccgtagcg cgccgcaatg tccgccatct gcgggagggg
     1981 atcgaccgcc gtcgtgcccg tggtgccgat gcaggctacg agggcacagg ggcgaagccc
     2041 ggcttccaag tccttggcga tagccgcctc caggagatcc aggcgaagcg cgtggttctc
     2101 atccgaggaa agcagccgca aatgtgcttt gccgaaacct gccagaagcg ctgccttctc
     2161 gatgctgctg tggccctggt ccgaggcgta gaccacgagg ggcgcgtcgc ccgactgtag
     2221 ccctttgcca ttctggctga agctggatgt ccgctc
//
LOCUS       DATMGX010000641         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_312519, whole genome shotgun sequence.
ACCESSION   DATMGX010000641 DATMGX010000000
VERSION     DATMGX010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312519"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..532)
                     /locus_tag="VNR65_10460"
     CDS             complement(119..532)
                     /locus_tag="VNR65_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ19136.1"
                     /translation="MPHLNGILETAIYVDDLRRAQRFYEDILELKPVFSDGRLTAYGV
                     GGRSILLVFQRGASIETATLPGGVIPGHDGAGPLHYAFAIGAEELPAWKERLNAHSIP
                     IEGCMNWPRGGKSIYFRDPDGHLLELATPGLWPID"
     gene            672..1712
                     /gene="moaA"
                     /locus_tag="VNR65_10465"
     CDS             672..1712
                     /gene="moaA"
                     /locus_tag="VNR65_10465"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWJ19137.1"
                     /translation="MTLQHSASNPIKPAGGAMVDPFGRHITYLRVSVTDRCDLRCVYC
                     MAENMTFLPRSDLLTLEELDRLCTAFIRKGVRKIRITGGEPLVRKNIMWLFSALSGHL
                     QSAALDELTLTTNGSQLKKFAADLAAAGVRRINVSLDTLDAQKFRAITRWGDLAQVMD
                     GIRAAQSACLQIKINMVALKNVNEHEIEKMLHWCHGEGMDLTLIETMPLGDIEGDRTD
                     QYLPLSVVRARLMDRLTLEDIPYRTGGPARYVRVRETGGRLGFITPLTHNFCESCNRV
                     RITCTGTLYMCLGQEDAADLRAPLRANASEELLGAAIDEAISRKPKGHDFVIDRRAQK
                     PAVGRHMSTTGG"
     gene            1716..>2066
                     /locus_tag="VNR65_10470"
     CDS             1716..>2066
                     /locus_tag="VNR65_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19138.1"
                     /translation="MRGWGRLVFVCLAFLFGPAIACLAEDGDTKLTPTETETLFLDRL
                     MRAESAGRQYAKNRASSALGPFQFLGTTFLDVARRNFPALASGKTDADILALRTDFQV
                     SRDVALIYTRENAGA"
BASE COUNT          416 a          637 c          594 g          419 t
ORIGIN      
        1 tgcttcccct gtcacgagcc tgtcaaagct cgcgacttcg tcttcacacg atacgcacct
       61 tgacgatcag tcacggtgcg attgcgttcc aggaaccttc tgggaagcga atctccatct
      121 aatcaatcgg ccaaagcccg ggcgtcgcga gttcaagaag gtggccatcg gggtcgcgga
      181 aatagatgct tttaccgccg cgcggccaat tcatgcagcc ttcaatcgga atactgtgcg
      241 cgttcaggcg ttctttccaa gcgggaagtt cttcggcgcc gatggcgaag gcgtaatgca
      301 acggaccggc gccgtcgtgg cctggaatca cgccgccggg aagcgtcgcg gtctcgatcg
      361 acgcgccccg ctggaaaacg agaagaatgc tccggcctcc gactccatac gcggtcaacc
      421 ggccgtctga gaagacgggc ttgagctcca gaatatcttc ataaaagcgc tgtgcgcgcc
      481 ggaggtcgtc gacgtagatg gccgtctcaa ggatcccatt caggtggggc atatctttcc
      541 tccctggctc acggtgaaag ccacgaatgc ttgtacaccc cacatcatga cagcttgttt
      601 ggcttcccta aataacgcat ctcatgtctt gccggggtgt taccctatca atcccagttc
      661 ggacgacgcc tatgaccctt cagcattccg cctcaaaccc tatcaagcca gccggcggag
      721 ccatggtcga tccgttcggg cggcacatca cctatttgcg ggtttcggtc accgatcgct
      781 gcgacctccg ctgcgtttat tgcatggcgg agaacatgac gttcctgccg aggtccgacc
      841 ttctcacgct cgaagagctc gatcgcctat gcacagcatt catccggaaa ggcgtccgga
      901 agatccgcat cacgggtggc gagcctttgg tgcgaaagaa tattatgtgg ctcttttcgg
      961 ccttgtcggg tcatctccaa agcgcggcac tggacgagct tacgctcacg accaatggca
     1021 gccagctcaa gaagttcgcc gccgatctgg ctgccgcggg cgttcgccgc atcaacgtct
     1081 ctctcgacac actcgatgcg cagaagttca gggccatcac ccgttggggc gacctcgcgc
     1141 aggtcatgga cgggattcgg gctgctcaga gcgcctgtct tcaaatcaaa atcaacatgg
     1201 tagcgctcaa gaatgtgaat gagcatgaaa tagaaaaaat gttacactgg tgtcatggcg
     1261 aagggatgga cctgacactg atagagacga tgccgctcgg agatatcgaa ggcgaccgca
     1321 ccgatcaata tctgccgctt tccgtggtgc gcgcgcggct catggatcgg ctgacgctgg
     1381 aggacattcc ctaccgcacg ggcgggcccg cgcgctacgt ccgcgtgcgc gaaaccggcg
     1441 ggcggctcgg cttcatcacg ccgctgaccc ataatttctg cgaaagctgc aaccgcgttc
     1501 gcatcacctg caccggcacc ctctatatgt gccttggcca ggaagacgcc gcggatttgc
     1561 gcgcgcccct tcgcgccaac gcctctgagg aattgcttgg cgcggcgatt gacgaggcca
     1621 taagccgcaa gcccaaaggc cacgacttcg tcatcgaccg gcgcgcccag aaacctgccg
     1681 tcggacgcca catgagcacg acgggcggat aagcgatgcg cgggtggggg cggcttgttt
     1741 ttgtttgtct tgctttcctc ttcggccccg caatcgcgtg ccttgctgag gatggcgata
     1801 cgaaactcac ccccaccgaa accgagacgc ttttcctgga tcgcttgatg cgcgcggaat
     1861 cagcggggag acaatatgcg aagaaccgcg cgtcgtccgc gctcggtccc ttccagtttc
     1921 tggggaccac gtttctggac gtcgctcgcc gcaatttccc cgccctagca tcagggaaaa
     1981 cggatgcgga cattctggcg ttgagaaccg atttccaggt ctcgcgcgac gtggcgctga
     2041 tctatacgcg tgaaaacgca ggcgcg
//
LOCUS       DATMGX010000642         4342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_230896, whole genome shotgun sequence.
ACCESSION   DATMGX010000642 DATMGX010000000
VERSION     DATMGX010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230896"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..904)
                     /gene="cpaB"
                     /locus_tag="VNR65_10475"
     CDS             complement(95..904)
                     /gene="cpaB"
                     /locus_tag="VNR65_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HWJ19139.1"
                     /translation="MNPRRLLVALLAALLLSGGVTYILTRRINRRPAPAQTTQKYVAA
                     SRPLQAGEVLKPENLTLVEWPAKMPLEGAFTKIEEVSGRAVIYPVATQQPVLEGYLAV
                     VGSGIGLTVKIPEGMRAASVRSDEIIGVAGFLFPGSHVDVLVTFRSDSIPTPATQIVL
                     QDVEVLTVGQKIEPEPGGKAENVGVVTLLLTPEDAQKIVLASTQGTIHFVLRNGADRG
                     RVAAVPVRVAEISSGARSPDTAPAARAHVTAKHAYEVETIIGDKHVTSQFE"
     gene            complement(906..1448)
                     /locus_tag="VNR65_10480"
     CDS             complement(906..1448)
                     /locus_tag="VNR65_10480"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HWJ19140.1"
                     /translation="MANLSTETVYLGAGVICAFSGAICDFRSRRIPNWLTGSSVLLGL
                     VLHLVLGGWHSVANALLAGLVAGGAFLLFYLAGGMGGGDVKLIAAVGCCAGLSHVTGI
                     LIATAIAGGIFALALALVAGRLKQTLSNVVQLLFHHGSAGLQPHPDLNVQNPRTLRLP
                     YGVAIGAGTAMTLFGALLGQ"
     gene            1483..1623
                     /locus_tag="VNR65_10485"
     CDS             1483..1623
                     /locus_tag="VNR65_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19141.1"
                     /translation="MRSDRLPGRQTPAWRTGEEAAPAVLQSAFSSVRQADGYGLTNLV
                     AR"
     gene            complement(1790..1954)
                     /locus_tag="VNR65_10490"
     CDS             complement(1790..1954)
                     /locus_tag="VNR65_10490"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HWJ19142.1"
                     /translation="MWKLLKNLRQDESGQDLIEYALIAALIALGAVVSMKAVTTAIGA
                     EFTKISAALT"
     gene            complement(2363..3112)
                     /locus_tag="VNR65_10495"
     CDS             complement(2363..3112)
                     /locus_tag="VNR65_10495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ19143.1"
                     /translation="MAPRRPELNHDKSGALAWIGDTIRSRGLFGALRCYAAGTVELLR
                     DLAPARRKSRYGDIEYDFEHGVDTTWATVSLRTRLRELLSGGQYQPSEPELFHEILNS
                     MPIAVDGFTFIDLGSGKGRTLLMASEYPFRRIIGVEILAELNEVAQQNIARYRSERQK
                     CSAIESQAGDARCFEFPREAIVLYLFNPFPEYVLREVLANLHRSFAALPREVYVVYHN
                     LVHESVFADRPWLQPVHRTQQFAIYKAVVRD"
     gene            complement(3180..3884)
                     /locus_tag="VNR65_10500"
     CDS             complement(3180..3884)
                     /locus_tag="VNR65_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWJ19144.1"
                     /translation="MITIRPSEERGHNHHGWLDTHFTFSFADYFDPKHMGFRDLRVIN
                     EDWISAGKGFGMHPHRDMEIITYMIEGELSHRDSTGRGAAIKQNDVQRMSAGAGVMHS
                     EVNQSKKPAHLLQIWILPEAEGLKPSYEDRTFPVEEKRNQLRLIASHDGREGSTTINQ
                     DTSVYASLLEDGQKLELELQPGRHAWVQLVRGELDVNGARLSKGDGAAISGETRLQLT
                     STATDGEAEFLLFDLA"
BASE COUNT          906 a         1274 c         1271 g          891 t
ORIGIN      
        1 cagctgaccc aacaggggaa cgcccgtcaa taaaatggcc atcaatacga ttcgcggcac
       61 ggttagtcca cgctttcttc ttccacgtac gctattactc gaactggctc gtaacatgtt
      121 tgtcacctat gatggtttcc acctcgtaag cgtgcttggc ggttacatgc gcacgggccg
      181 cgggtgcagt gtccggggag cgtgccccgc tcgaaatctc agccacccgc accggcaccg
      241 ctgccacccg cccgcgatcg gccccattgc ggagcacgaa atggatggtg ccctgggtgc
      301 tcgccagaac gatcttctgc gcgtcctccg gcgtcaacag caaggtcacg accccaacgt
      361 tttccgcctt tcccccaggc tcgggctcta ttttttgacc caccgtgaga acttcgacat
      421 cctgcagaac gatttgcgtg gcaggagtgg gaatcgagtc gctacggaag gtgaccagca
      481 catccacatg agaaccgggg aacaggaatc ctgccacccc tatgatctcg tcggagcgga
      541 ctgaagcggc ccgcatacct tcggggattt tcaccgtgag acctattccg gagcccacta
      601 ccgccaaata tccttcgagg accggttgct gcgtggctac cggatagatc acggcccggc
      661 cggagacctc ttcgatcttg gtaaacgctc cttcaagagg catctttgcc ggccactcca
      721 ccaacgtgag gttctctggc ttgagcactt cgccggcctg caaagggcgc gacgcagcca
      781 catatttctg ggttgtttgg gcaggggcag gcctccggtt gatccggcgg gtgagaatgt
      841 aggtgactcc gcccgagagc aacaaggcgg ccaaaagggc aacaagaagt cggcgggggt
      901 tcatattatt gacctagtag ggcaccgaat agagtcatgg cggtgcccgc tccaatcgcg
      961 acgccgtaag gcagtcgaag agttcgggga ttttggacat tcagatcggg atgcggctgt
     1021 agaccagccg atccatgatg gaacaacagt tgcaccacat tagaaagagt ctgtttgagc
     1081 cgccccgcga ccaaggcgag agcgagggcg aagatcccac ccgcgattgc ggttgcgatg
     1141 aggatcccgg ttacatggct taggccggca cagcagccga cagcggcaat cagcttcacg
     1201 tcgccgccgc ccattcctcc ggcaaggtaa aagagcagaa atgccccgcc tgcgacaagg
     1261 cctgccagta aagcattggc gacggaatgc catcccccca ggacaaggtg cagcaccagg
     1321 cccagcagga ccgaggaacc ggttagccag ttaggaattc ggcgagagcg aaagtcgcag
     1381 atcgcgccac taaacgcgca gataacgcct gctccgagat ataccgtttc cgtactgagg
     1441 ttcgccacgt ttaagacacc ttaagtttgg ggacgagcga ccatgcggtc cgatagactg
     1501 ccgggccggc aaactccggc ttggcgtacc ggcgaggaag cggcccccgc cgttttgcag
     1561 tccgcgttca gttcggttcg ccaggctgac ggctacggcc tcacgaacct ggttgcgcgg
     1621 tgacgaccag gtccaccacc gcacaaccca accacttgag agccttaact tatctcctta
     1681 cgcgtttccc gcctgtatgg cccggttcag gcgagcgtgt tggtgagttg ggtgttgatt
     1741 gtcgcaaacg cactggcaat cgcgccggcg acgactttca tgctgcctac tacgtcagcg
     1801 cggcgctgat tttggtaaat tcggccccaa tcgcagttgt gaccgccttc atgctgacta
     1861 cggcgcccag ggcaatcagg gcagcgatga gagcgtactc gatcaggtcc tgaccggact
     1921 cgtcctgtct caggttcttg agaagtttcc acataatgca tcctccaaat acgggctttg
     1981 tctaaaactg cacatgctac tgtgctgcta tacctatcgg actagcggcc aaataacttg
     2041 agccgcgctc tggccgcacc cgacgttgcg agaaagctct cgccttcgcc atctcacctc
     2101 agttatggga aaacgggttg aagaacgggc tttatgggca ggacctggcc ttcgggaccc
     2161 ttatgccagc gtggttaggc cgatgctgtg cattgcgtca cggctgtccg gggaaacttt
     2221 cgcgatgaac gcgcaagcgg cgcagattga gcatgataca tccgctttgg aacaaaaacg
     2281 atttgccacg cggaagcgcg gtgaaggcgt gcttggcggc attgttcgga gcataggtcg
     2341 ccaacatcga gttcaactgc catcaatcgc ggaccaccgc tttatagatc gcaaactgct
     2401 gcgtgcggtg gacaggctgc aaccatggcc gatcggcgaa gacactctcg tggaccaggt
     2461 tgtgatagac gacataaacc tcgcgcggaa gagcagcgaa agatcgatgc aggtttgcca
     2521 gcacctcgcg cagcacatat tcgggaaagg gattgaacag gtaaagtacg atggcctcgc
     2581 gtgggaattc aaagcagcgg gcgtcgccgg cctgtgactc gatggcggaa cacttctgcc
     2641 gctcgcttcg gtagcgcgcg atgttctgct gcgcgacctc gttcaattcc gcaagaattt
     2701 ccacgccgat gattcgccgg aagggatact ccgatgccat cagcagggtt cgtcccttgc
     2761 cggagccgag atcaataaac gtaaacccat caaccgcgat gggcatggaa ttgagaatct
     2821 cgtgaaacag ttcgggttcc gagggttgat actgccctcc ggaaagcaac tcgcgaagac
     2881 gagtgcgtag gcttacggtt gcccaagtcg tgtccacgcc gtgctcgaag tcgtattcga
     2941 tgtcgccgta gcgcgatttt cggcgggctg gcgcgaggtc gcgcaacaac tcaactgtgc
     3001 cagccgcata gcagcgcaag gcgccaaaca ggccgcgact gcggattgtg tcgccgatcc
     3061 acgccaacgc accggatttg tcgtgattga gttctggtct tcgcggcgcc acaggaagat
     3121 agtaatcggg cgcggcacga tgcgacagta tcgcgcatcg cgccgcagcc gcactggaat
     3181 tacgcgagat cgaacagcaa gaattcagcc tcgccgtcgg tggcggtgct ggtcagttgc
     3241 agcctggttt cgccgctgat cgccgcgcca tcgcctttgc tcagcctggc gccgtttacg
     3301 tccagttcgc cgcgcaccaa ctgcacccat gcgtgccggc cgggctgtag ttcgagctcc
     3361 agcttctggc cgtcttcgag cagcgaagcg tacacgctcg tgtcctgatt gatggtggtc
     3421 gagccctcgc gcccgtcatg cgacgcgatg agtcgcaatt gattccgctt ctcttcgacc
     3481 gggaaggtgc ggtcctcgta gctgggcttc aacccttccg cctcaggcaa aatccatatc
     3541 tgcagcaggt gcgccggctt cttggactga ttgacctcgc tatgcatgac accggcaccg
     3601 gccgacatgc gctgcacgtc gttctgcttg atagcagctc cgcgtcccgt gctgtcgcga
     3661 tggctcagtt cgccctcgat catgtaggtg atgatctcca tgtcgcggtg gggatgcatt
     3721 ccgaaaccct tgccggcact gatccagtcc tcattgatca cgcgcaagtc gcgaaaaccc
     3781 atgtgcttgg gatcgaagta atcggcgaac gagaacgtga agtgcgtgtc cagccagccg
     3841 tggtggttgt gtccgcgctc ttcggacggc ctgatggtga tcatgatgtt cctcctgaat
     3901 ccggtatcca taagatgctt gaacgttaaa gaaagattcg gaactggcaa agcggctaaa
     3961 gcaattccag agtggttgta ccttgggtag tgcgggcctg aaacccggat tattcatgaa
     4021 cttgaggatg tcatcctgag cgaagcgccc gcaaagactt tccgacccgc ttcttttgcg
     4081 ggtcgggcgg gcgcgcagtc gaaggatctg cggttgttcc cgacggcgta acgataccag
     4141 cgaaccgcag atccctcgac tcgggccgcg cagcgcatcc agcaaaacgc aggctggatg
     4201 cgtttttggc gcggccctcc ctcgggatga caatattatc gtttgttcct tatgaataat
     4261 ccggactagt actacagttg tagatagaat gcgggcagag gcgatccgcg ctttcgatag
     4321 tggcagcaca tagctcggaa tt
//
LOCUS       DATMGX010000643         3566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_183861, whole genome shotgun sequence.
ACCESSION   DATMGX010000643 DATMGX010000000
VERSION     DATMGX010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183861"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VNR65_10505"
     CDS             <1..259
                     /locus_tag="VNR65_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWJ19145.1"
                     /translation="DTPFDCGAVATALVNAAWSRGLGAVINSQGIMQSPVVREHAGIA
                     DDQVIMKSIALGWPDDSFPANAVVSERKSVDEATVFVGFDN"
     gene            540..737
                     /locus_tag="VNR65_10510"
     CDS             540..737
                     /locus_tag="VNR65_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19146.1"
                     /translation="MPGETILDILRADEAMNTWFVVMIILLGVGALTLHRYDVQRVLI
                     WIAFGWFVLGALLLGYRGEHL"
     gene            complement(1463..1876)
                     /locus_tag="VNR65_10515"
     CDS             complement(1463..1876)
                     /locus_tag="VNR65_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ19147.1"
                     /translation="MINEQGRINERKINLIQYDDAYSPPKTVEQVRKLVESDEVFTTF
                     QIIGTPPNAAVQKYLNEKKLPQLLASTGATRFTDPKNFPWTIAFNPNYQSEAHIYAKF
                     ILDNHPNCRQRTFGYPRHAPSFVSTARRRSPWVKV"
     gene            2004..2375
                     /locus_tag="VNR65_10520"
     CDS             2004..2375
                     /locus_tag="VNR65_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19148.1"
                     /translation="MGRHASFDSDQAPRHIGEPGCDPAAGDLLPQNDCPPVVEADQMQ
                     GVLARIDADGVCACSGCLMGHSDVLLVLLSPRSFSERFWAGARPVHPVLGCRTAEAAL
                     LRIRHGLLYEIKFLAGSAKLG"
BASE COUNT          719 a         1005 c         1036 g          806 t
ORIGIN      
        1 cgatacgccc ttcgattgcg gcgccgtggc gaccgccctg gtcaatgccg cctggtcccg
       61 cgggctgggc gcggtgatta acagtcaggg catcatgcaa tcaccggtgg tgcgtgagca
      121 cgccggaata gctgacgatc aggtcatcat gaaaagtatc gcgctgggtt ggcccgatga
      181 cagcttcccg gcaaatgcgg tcgtgtccga acgaaagtct gtcgacgaag ctacggtgtt
      241 cgtcggtttc gacaattagg cgcgcggcct attggttgcc aaaagggcac tggcggccac
      301 ggtcgcgcga accgccgttg ccttcgagtc tatcaccttc atcgtcggcc ccccgtgaaa
      361 tgcaaaaagg cgacgctgaa ttggcgccgc ctagttacgt gatcccatag tcgtttgagc
      421 catgccacat ggcccatcac tcccgcactg tcggcgcgat gcatagcgcc cacatagcgt
      481 gggcgcaatg tgttaatctt tccatcggag cgaacgggat tcttaatctc gtagctcaca
      541 tgcccggcga aacgatcctc gatatccttc gggcggatga agccatgaac acctggttcg
      601 ttgtcatgat tatcctgctc ggggtcggcg cgctgacctt gcacaggtac gacgtgcagc
      661 gcgtcctgat ctggatcgcg ttcggctggt tcgtgctggg cgcgctcctg ctcggttaca
      721 ggggcgagca cctgtgatct cacagccgag cttgcagtct gcacattagc gctatgcaga
      781 caacgacgcg ccgtttcgat ttcggcatcg ctggcgccgt gacctcgtgc ccacgtttct
      841 gcggctgatt gcgagtattt ggcgacatag aaacgaacaa cggcgcaaga cggcacgcct
      901 gctgtggccg tttggctgag tgcaatcaaa gttgcaaagg ctgggatcgc tcgtctcatc
      961 atcatcacga tccaccgtga aggaacgcag caatttcgaa cgtctctgtt tttggttcgg
     1021 ctccgccatc ggtcttcgga tgaaggtgcg cggattccac tggctaggca tttctggcac
     1081 ccatccagct ccgccgtcca gtcactggcg gagagcgcag cgacaaatcc agtccgaacg
     1141 tttacaagtt atctatagaa tcgagcaaat gatgtattga tttacccgac ttattcccac
     1201 gaaagttcat tcaaacactt ttcggaaacc tctcgcaatg catgcaagtc tcggccttga
     1261 agggttggtg ttgaagcggc gcgaccggcc ttatcagctg gccggtcgaa ggactgggta
     1321 aatgtgaaga accggaagca cccggcgagg tgcttgggtg agaccagaca caccgtgttt
     1381 gttctaatag cgcgcaacag gacgaggagc gtcgaaaaat ctgcgcaccg agagaaccct
     1441 ccctcggaaa cgaaagcgtc ccctagactt taacccaagg agaccgtcgc ctcgccgtcg
     1501 aaacaaacga cggcgcatgt cgcggatagc caaaggtcct ttgtcggcag ttcggatggt
     1561 tgtcgagaat gaatttggcg tagatatgtg cctccgactg gtagttgggg ttgaaagcga
     1621 tcgtccacgg gaaattcttc ggatcggtga agcgcgtcgc gccagtcgag gccagaagct
     1681 gcggtagctt tttctcgttg agatatttct ggaccgcggc atttggcggc gtgccgatga
     1741 tctggaaagt ggtgaaaact tcgtcacttt cgacaagctt gcgtacctgc tcgaccgtct
     1801 taggcggact gtaagcatcg tcgtactgaa tgagattgat ctttcgctcg ttgattcgac
     1861 cctgctcgtt gatcattcgg atataggcgg cctacacctt gccgatgctg ccgtaggcgg
     1921 aagccggtcc ggaacggttt ggcataagct ggtgggacct gccttacgtc atgaccgagc
     1981 gcaattagtt cgcgagccac gtagtgggtc gccatgccag cttcgattcc gatcaggcac
     2041 cgcggcacat tggcgaaccg ggctgcgatc cggccgcggg cgatcttctc ccgcaaaacg
     2101 attgcccccc tgtcgtcgag gccgatcaaa tgcagggtgt tcttgcccgt atcgatgccg
     2161 atggtgtgtg cgcctgtagc ggctgtctta tgggacatag cgatgtgctc ctcgtgcttc
     2221 taagcccccg ctcattctct gagcggttct gggcgggagc acggccggtc catcccgttc
     2281 tcggatgtcg caccgctgag gcggcattgc tgcgcatccg gcacggcctc ttatacgaga
     2341 tcaagttcct cgcgggatcc gccaagctcg gctagttcct tcgccaacca atgcaattca
     2401 gctgtccggt cgagcaacgt agcttttgcg gtccgatcaa cagtcctctt ggcctgctgt
     2461 tcgcactcgt cggcagctct tcgcaacgct tcgaacgtct tggtcatctc gatgctcacc
     2521 gttacctgct agatagtctc gctttgaatg tggaagctta caactagggc acaatacgga
     2581 ccgggaaagc acgggtcacg taattcacgc ccccttaagc ataaaccgac gacaattttt
     2641 ggtgtctgaa gggattgtca tgacacacaa agaccaatgc gcatcgtgcg gttcagaaat
     2701 ggcgttcgtc cacctgaagc gtgttgcaaa agggtttgcg atggaaacta ccgaatgccc
     2761 gaaatgcagg ttcgcgatga acctcgtagt gaggcatcgg tttccacgag ctgcgactct
     2821 cgcccgccgc ttggccaacg agcgcgcagc gtagtgagga ataaagaagc aagctgccat
     2881 cccaacgcgt ggctggatac cgtgccttct ggcaaggaca cgtcgcacgc agaatttagc
     2941 gaacgtggag ttcagtaggc atattcctcg caacgtatcc gctgcaggtg cagcattgga
     3001 tctaaattgc ggaatggcca gaggatcgcc tctgccttcc cgggtaattt ggcgcggcaa
     3061 aagacgactg ggtgttgctt tcgaatgagg cgggcgaccg tccagccgat gtcgcacttt
     3121 ggcaccgaac ggacgcaccg cgcaggtctg ttgatgtccg ttgatcgggg tgatccggaa
     3181 gtcgcatgct aaggccgcca agaccgtttt tgaccctgag cggatatcgg cctcgcgtcg
     3241 gataacgaga cattcacgcc tatcaatacg ccaccgacgt ccctttatct tggagctctt
     3301 agcatgagag caaattgggc agcttccgcg cctctgttta ttcttgtctg gtagcgcaca
     3361 aacccatttc gcagcgacct gctgccggct gtttgtgtag cggtctcagt ctctgctcga
     3421 cggcgctgcc gtgggggtct ttacagaacc tctatttata cggttcacgt ttcggcgcgt
     3481 gacgcctcgc acgggctctg ccgggcattc aaggccgagg gagagcctga gcgcacgcat
     3541 ttggctgggg gacaccgaag ttccta
//
LOCUS       DATMGX010000644         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_113617, whole genome shotgun sequence.
ACCESSION   DATMGX010000644 DATMGX010000000
VERSION     DATMGX010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113617"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VNR65_10525"
     CDS             <1..255
                     /locus_tag="VNR65_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19149.1"
                     /translation="PPDAGHLRHPGGADLHLGKDDRVAAAWMGGVERDGSAVCCGVKM
                     AMLVTLVFPFIILVFTATSVLKGFGTSSIANCCCNEKMSY"
     gene            complement(286..1545)
                     /locus_tag="VNR65_10530"
     CDS             complement(286..1545)
                     /locus_tag="VNR65_10530"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HWJ19150.1"
                     /translation="MSQKEFQRVKVIENAAGGRLSVREASRLLQVSERQVQRLKRRYR
                     PDSIGWVRHGNRGRSMPWALPLPQKQLILNLARGKYQGFNDSHLAEKLRAEENLAVSR
                     ETVRRILRAAKLSSPQQRRPRQYRSRRPPRPRFGMMALTDASRHDWLEGRGPVLTLLG
                     FQDDATGQILAAHFQLEPENTFGYLRALHAMIATHGVPLSLYRDRHSIFQRNDPHWTL
                     AEQLAGKQSPTQLGRALEELGIEQIPAYSPQAKGRIERAWRTCQDRLVSELRLARATT
                     LPEANAVLARFCADYNQRFARPAAESARDFRSLPRPFDLARCLALHYQRVVAADHTVT
                     LGAHSIALPPLPGHRGYAGETVELAHHLDGMLRVYRGDQLLLALPLPLEEHAERRPKL
                     LTSAQKRKTTLPRIYNLSGRPALAAVT"
     gene            1602..1985
                     /locus_tag="VNR65_10535"
     CDS             1602..1985
                     /locus_tag="VNR65_10535"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWJ19151.1"
                     /translation="MEHLHSSIANPGPHGFSEILYGFVSTAGNNGSAFGGLNGNTPWY
                     NTTLGLNTLIGRFFMIIPMLAIAGNLARKQAVPPSAGTFPVTTPLFTVLLVGVIVIVG
                     ALTFFPALSLGPILEHLLLHAGKAF"
BASE COUNT          382 a          534 c          708 g          415 t
ORIGIN      
        1 cctccagatg ctggccatct tcgccatccc ggcggggctg acctacacct tgggaaagat
       61 gaccgggtcg cagcggcatg gatgggcggt gtggagcgcg atggctctgc tgtttgctgc
      121 ggggtcaaga tggcaatgct cgtcacgctg gttttcccct ttatcatcct ggtgtttaca
      181 gcgacctcgg ttctgaaggg attcggaacc tccagcatcg cgaactgttg ttgcaacgag
      241 aaaatgtcgt attaactgca acgagactct gtcaccccct gtcgattaag taacggccgc
      301 taaagcgggg cggccggaga ggttgtagat gcggggcagg gttgtttttc ttttctgcgc
      361 cgaggtgagg agcttgggac ggcgctcggc atgttcttcg agcggtagag gcagcgcgag
      421 aagaagctga tctccgcgat agacgcgcag catgccatcg aggtgatggg cgagctcgac
      481 cgtttctccg gcatagccgc gatggccggg caagggtggc agggcgatgg agtgggcgcc
      541 caaagtgacg gtgtgatcgg cggcgacgac gcgttgatag tgcaaggcca gacagcgggc
      601 gagatcaaag gggcgcggca aggagcggaa gtcgcgagcg gattcggcgg cgggacgggc
      661 aaagcgctgg ttgtagtcgg cgcagaagcg ggcgagcacg gcattggcct cgggcaaagt
      721 ggtggcacgg gcgaggcgaa gctcgctgac cagacggtcc tggcaagtgc gccaggcacg
      781 ctcgatgcgg cctttggctt ggggggaata ggcggggatc tgctcgatgc cgagttcttc
      841 gagtgcgcgt ccgagttggg taggagattg ttttccggcg agttgttcgg cgagggtcca
      901 gtggggatcg ttgcgctgga agatggagtg gcggtcgcgg tagaggctga gcgggacgcc
      961 atgagtggcg atcatggcgt ggagggcgcg gagatagccg aaagtatttt cgggttcaag
     1021 ctggaagtga gcggcgagga tctggccggt ggcgtcgtcc tgaaagccga ggagcgtgag
     1081 cacggggccg cggccttcga gccaatcgtg acggctggca tcggtgaggg ccatcatgcc
     1141 gaagcgtggg cggggcgggc ggcgggagcg gtattgacga ggccggcgct gctgcggaga
     1201 agaaagtttc gcggcgcgca agatgcggcg cacggtctcg cggctgacgg caaggttctc
     1261 ttcggcgcga agtttttccg ccagatgcga gtcgttgaag ccctggtact tgccgcgggc
     1321 taagttcaag atcaactgct tctggggaag aggcaaggcc caaggcatgg agcgtccgcg
     1381 attgccgtgc cggacccagc cgatggaatc gggtcgatag cggcgtttga ggcgctgcac
     1441 ctgacgttca ctcacttgca gcagacggga ggcttcgcgt acgctcagcc tcccgcccgc
     1501 tgcattctca atcactttca cccgttggaa ctctttctga ctcatcgtca ccatccttca
     1561 agggtgacaa agtctcgttg cagttatggg gtgacattat cttggagcat ctacactcca
     1621 gcatcgcgaa ccccggacct cacggatttt cggagattct gtacgggttt gtttccaccg
     1681 cagggaacaa cgggtcggcc ttcggcggtt tgaatggaaa tactccctgg tacaacacga
     1741 ccctggggct caacacgctg attggccgct tcttcatgat cattccaatg ctcgcgattg
     1801 ccgggaatct cgcacgcaaa caagccgtgc ctccatcggc cgggaccttt ccagtgacaa
     1861 caccgttgtt caccgtgctg ctggtgggcg tgattgtcat cgtaggtgct ctgacgttct
     1921 tcccggcgct gagtctcggt ccgatattgg aacacttgct gctccacgca ggtaaggctt
     1981 tctaacccaa tgagcaaatc aaaccaatcg aaacgggcag tctgggacgc acgcatcgt
//
LOCUS       DATMGX010000645         3893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_163226, whole genome shotgun sequence.
ACCESSION   DATMGX010000645 DATMGX010000000
VERSION     DATMGX010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163226"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            389..1126
                     /gene="aqpZ"
                     /locus_tag="VNR65_10540"
     CDS             389..1126
                     /gene="aqpZ"
                     /locus_tag="VNR65_10540"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HWJ19152.1"
                     /translation="MRKFFAEFIGTFWLVFGGCGSAILAAKFPEVGIGLLGVALAFGL
                     TVLTMAYAIGHISGCHLNPAVTIGLTAGGRFPIGQALPYIVAQVLGGICAAYILFAIA
                     SGGPGFDLSQGFAANGYADHSPGKYTLFSGFLSEFILTMMFLFIIMGATHGKAPVGFA
                     PLAIGLGLTLIHLIGIPVTNLSVNPARSTGPALVVGGWALQQLWLFWLAPILGGIAGG
                     LLYRLAVDEPAAQVVGFDETRLKRSRA"
     gene            complement(1324..1776)
                     /locus_tag="VNR65_10545"
     CDS             complement(1324..1776)
                     /locus_tag="VNR65_10545"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWJ19153.1"
                     /translation="MATPFTLSQQSNLQPIAPNLSVHSAGDSNISNDLTIIGDVSSSG
                     SVTLDGIIEGNIYCTSLIVTANGRVNGGIVANQEVTVQGKVSGTIRGRRVMLQSSAKV
                     DGDIFHQGIGIEMGTRYDGTLRWTEDEHVFDEPSFPAPGKQENGDYHS"
     gene            2313..3506
                     /locus_tag="VNR65_10550"
     CDS             2313..3506
                     /locus_tag="VNR65_10550"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HWJ19154.1"
                     /translation="MRAGFFSKWGAIAAIAILVAVQAAAINYLGRNNYLPEGSEHWTE
                     DWLIHYFSKRLDQPHKNIALVMVDAESLEKSSLPATVPVDRGWMAELITSVSERGPAA
                     IGLDFYFTSAIDPIKDEQLVAAIRDSKTPITLAAVNDEFLRTDSQRSFQRAFHQKVNR
                     PAGHIYLQQSKEIFTLGDRATRGVDHGLAGGRYRSFSSTLARLPEVEAVFGPRRIPDG
                     VQRIDWLLAPEGGETFTRIPAHEILSQGDAANPASLKGKIVLIGPDFAGLDQHSVPFS
                     LGSQQDLYPGIFVHAQALAQILDKRFFFNWNSEEQFFLLFVIGLLGAAAGWAFHNTRA
                     DLVIGVGGSLLIIALSIPFFIAHVPLPTALAILSWALSISIAQRIRSWRESSHSQVKT
                     FQGAG"
BASE COUNT          777 a         1119 c         1088 g          909 t
ORIGIN      
        1 aaacgtcagg aataaattga cacacagatt ccgccatacg cagtttgtat acaaaaatct
       61 ggtcgctcgt gtgcgattaa gcgcacgatg ctccagagca gatttgcggc tcagacatga
      121 gcagggccga tttatccgat attcggactg cctgggttca atccgcctcc cactcctttc
      181 agatctgctg tcaagcctcc gaagtggtgg agtgatccgg atcgcctttg tgcccggcaa
      241 tgaatgggag gtcgcggcgg tgaaggcgtt gctctctgga taaggaatca gtgatgagcg
      301 catcagccaa aaatgcaata taacgctcat gggcgtaagt taaggaatgg cgccgaaaag
      361 ggatgaaaca cagaggcaag gacggcacat gcgcaaattt tttgctgagt tcatcggaac
      421 attctggttg gttttcgggg gatgtggcag cgcaattctt gcggccaagt ttccggaagt
      481 cggaataggc cttcttggcg ttgcgctagc ctttggctta acagttctca ccatggccta
      541 tgctattgga catatctccg gatgccactt gaatcccgcg gtgacgatcg gcctcacggc
      601 cggaggacgc ttcccgatcg gacaggctct cccgtatatc gtcgcgcaag tccttggcgg
      661 catttgcgct gcttacattc tttttgcgat cgccagcgga ggacccggtt tcgacctgtc
      721 tcagggcttc gccgcgaacg gctatgcgga ccattcgcca gggaagtaca cgctcttttc
      781 cggtttcctg tcagagttta tcctcacgat gatgtttctg ttcatcatca tgggcgcgac
      841 acacgggaag gcgccggtcg ggttcgcccc cctcgcgatc ggcctcggtt tgactctcat
      901 tcatctcatc ggcatccccg tcaccaacct ctccgttaat ccggcacgca gcaccggacc
      961 ggctctcgtt gtcggcggct gggctcttca gcagttgtgg ctattttggt tggcgccaat
     1021 tctcggcggc atcgcgggcg gccttctcta caggcttgcc gtcgacgagc ccgcagcgca
     1081 agtcgtgggt tttgatgaga cccgcttgaa gcggtcccgc gcctaagtcg cgaaggcgtc
     1141 atcccggaag ggcggagccc tatccgggat cgcaacgcat tgaaacgctt aattttttgt
     1201 gatcccggct cggcgcttcg cttagccggg atgacgaagg gctcaagtga ggcgtccgcg
     1261 atccgttgct agattggcga agtcagctag cagcaactgc gcatggctcg tttatcagat
     1321 tgatcagctg tggtagtcgc cgttttcctg cttgccgggc gctggaaagc tcggctcatc
     1381 gaaaacgtgc tcatcctccg tccagcgaag cgtcccgtcg taacgggtgc ccatctcgat
     1441 gccgattccc tggtggaaaa tatcgccatc aaccttcgcg gaagactgca gcatcacgcg
     1501 gcgtccgcgg attgtacccg acaccttacc ctgaacggtc acttcctgat tggcgacgat
     1561 accgccgttc acgcggccgt tcgccgtaac gatgagcgac gtacaataga tattgccctc
     1621 aatgatccca tcaagggtga cactgcccga ggacgacaca tcgccgatga ttgtcagatc
     1681 gttactaatg ttcgaatcgc ccgccgagtg cacgctgagg ttgggtgcga tgggctgcaa
     1741 attggactgc tgcgaaaggg taaacggagt cgccatggct gaccttcaag cgtaacaaca
     1801 cggtattaaa cccagagcgg gaccggccgc gggttaggct gccgttctct tctctctagc
     1861 ccgtcattcc aggcatttgc aaggcaaagc tccgcgcaat gcccaggcta cttgcgttcg
     1921 gcgagctcgc aggctgagag cctgagtaaa tatctcggcg cactccaaac acagtttcca
     1981 gacgtttcca aataggaaca atcatgagtt gattgaggtg caaccttagg ttaaacgcct
     2041 gacggcgttg gttcacgcgc tagagcctga tgtcgagttg aagcatcggg tcgtggagaa
     2101 cttggctgcg gatttccttt aatccgacga gaattatacg ccccctacga gaaggcgctg
     2161 aatagcaaga gtatattacg ctgggttgac gctcgcgagc gcgagcgtgc ccgacatatt
     2221 tgcggtggcg acccttccat cccggcgttg ttctgttgaa atgagcattc tggagcttgc
     2281 gctttccggg ccgttcgata aaagcgcaag ctatgagggc cggttttttc tcgaagtggg
     2341 gcgctattgc tgcgatcgcc attctggttg cggtgcaggc tgccgccatc aattaccttg
     2401 gtcgcaacaa ttaccttccc gagggctccg agcattggac cgaagattgg cttatccatt
     2461 atttttcgaa gcgtctcgat cagccccata agaatatcgc tctcgtcatg gtcgatgcgg
     2521 agagcctcga aaagtccagt ctcccagcga cggttccggt tgatcgggga tggatggcgg
     2581 agcttatcac gtcggtatcg gagcgggggc cggctgcgat cggcctcgat ttttacttca
     2641 cgtccgcgat tgatcccata aaagacgagc agcttgtggc cgcaattcgg gattcgaaga
     2701 ccccgatcac gctcgccgcc gtgaatgacg aatttctgcg cacagacagc cagcgcagct
     2761 ttcagcgcgc attccatcag aaggtgaacc ggcccgccgg acacatttat cttcagcagt
     2821 cgaaagagat atttacgctc ggcgacaggg caacgcgagg cgtggaccac ggcctcgccg
     2881 gaggccgcta tcgatcgttt tcgagcactc tcgcgcgctt gcctgaggtg gaagccgttt
     2941 tcggcccgag gcggatcccc gatggggtgc agcggatcga ctggcttcta gcgcccgaag
     3001 gcggcgagac cttcacccgc atccccgcgc acgaaatcct ttcccaaggc gatgctgcaa
     3061 atcccgcttc cctgaaagga aaaatcgtcc tcatcgggcc agatttcgcc ggcctcgatc
     3121 aacatagcgt tccattttcc cttggaagcc agcaggatct ctatcccggc atttttgtac
     3181 atgcccaggc tctcgctcag attctcgaca agcgattctt ctttaactgg aactcagagg
     3241 agcagttttt tctgcttttt gtgattgggc ttctgggagc tgccgcaggc tgggcatttc
     3301 acaacacgcg cgccgatctc gtcattggag tcggcggttc tcttttgatc attgccttaa
     3361 gcataccctt ttttattgca catgtgcctc ttccaacagc gttggcgatt ctttcctggg
     3421 ctttatcgat ctcgatcgct cagcgcatcc gttcatggcg agagtcctcg cattctcaag
     3481 ttaagacctt tcagggagca gggtgacatg aaatttccag ccttcgtttt tctcgctctg
     3541 tcagcggcat tcttaggaac cagcgtatgt caggcgcagc agcatacagt cgaaaaggcc
     3601 ccgccggctg tggctgtccc cgcccctgcg tctcagcccg ccccagccca tcactcaagg
     3661 tcgttgcaga aagctcctgt cttcgaagcc ccggctcccg cgccagcgtc gaaacctgat
     3721 tccggcaatc ctgatgtcgt cgttggcggc ctgccaggtg caaaatccgc gaccgcgggc
     3781 gagaagcaac cttaggtcag ccaaggctta gcttgcaggc ccgcgcgact ttcgcgccaa
     3841 agaggaggag tgcgccttta cgccgcgccg cgacttgttt agaaaatcgg tcg
//
LOCUS       DATMGX010000646         2685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_476769, whole genome shotgun sequence.
ACCESSION   DATMGX010000646 DATMGX010000000
VERSION     DATMGX010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476769"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VNR65_10555"
     CDS             complement(<1..810)
                     /locus_tag="VNR65_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ19155.1"
                     /translation="MASWRVGSLILCAFFGCCISGAFGQEAAKVKIGFLRTTEQRTTI
                     SLIEQPSPNDGLAGAELAVDDNNTTGRFLNQSFSLGDVKLRPQDDAATAVNALADQGV
                     SLVIADLPPDQLLKAADAGRAKGMLLFNAGATDDRLREEDCRANVIHVAPTRSMLADG
                     LAQYLVWKSWRKWFFVVGSHAKDKLWGDALKRSAKRFGAKVVDERTFADTGGARQTDS
                     GIAQIQRQIPSFTQGAPDYDVLVTADESEVFAAYFPYRTYAPRPVAGSAGLV"
     gene            961..1476
                     /locus_tag="VNR65_10560"
     CDS             961..1476
                     /locus_tag="VNR65_10560"
                     /inference="COORDINATES: protein motif:HMM:NF023113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HWJ19156.1"
                     /translation="MTLRFPILLLGVLALFAWQPVATAAQRVAVFDFELIDTSLEGET
                     YGTRADEKERLKKLSDQLRQALAKSGQFEVVDIGPVEAAAQGANLQACGGCDGTLAQK
                     VGADLSITGTVQKISNLILNMNIYVRDVSSQKLIASRSADFRSNTDESWSRTLKYIVK
                     NYLLAPNFGSN"
     gene            2041..>2685
                     /locus_tag="VNR65_10565"
     CDS             2041..>2685
                     /locus_tag="VNR65_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWJ19157.1"
                     /translation="MTLAKMRFAASVVGLCVTALWYDAAAQDMLQYLDLNSPDMTRAE
                     MTRSDVEAMIKAAQGNALDLSGKRLSGLDLSGLDLTRANLSSTRLNNTSFAKARLDGA
                     LLDLSWALKANFSGASLKGASLFSAQMQGSKFDHADLSGARIAANLTGASLRDANITG
                     ANGSPDMKNQSMGLMRTVLKSANLEGADLSGSNLTRVDLAFANLQGADLSGANLR"
BASE COUNT          594 a          767 c          749 g          575 t
ORIGIN      
        1 aacgaggcca gcagaacccg ccacgggtcg aggtgcgtat gtccggtagg gaaagtaggc
       61 cgcaaagacc tcgctctcgt cagccgtcac gagcacatcg taatccggcg caccttgcgt
      121 gaaggatgga atctggcgct ggatttgtgc gatgccgctg tccgtttgcc tcgcgcctcc
      181 cgtgtcggcg aaggttcgct cgtccacgac cttggccccg aaacgttttg cggaccgctt
      241 cagcgcgtcg ccccaaagct tgtcctttgc atgcgagccg acaacgaaga accacttgcg
      301 ccagctcttc cagacaagat attgcgcgag cccgtcggcc agcatcgagc gcgtgggcgc
      361 gacgtgaatg acgttggcgc ggcaatcctc ttctctcagc ctgtcgtctg tcgcgcccgc
      421 gttgaaaagc agcatccctt tggcgcgtcc cgcatcggcc gccttcagca gttggtcggg
      481 gggaagatcg gcgatcacca acgacacgcc ctgatcggct agcgcattga ctgccgtcgc
      541 cgcatcgtcc tgcggcctga gcttgacgtc acccagagag aaactctggt tcagaaagcg
      601 gccggtggtg ttgttatcgt cgacggcaag ctccgcgccc gccagcccat cattgggcga
      661 gggctgctcg atgagcgaga tcgtggttct ttgctctgtc gtccgcagga agccgatctt
      721 gaccttcgcc gcttcctgcc cgaaagcccc cgagatgcaa cagccaaaaa aggcgcagag
      781 gatcaagctg ccgacgcgcc aacttgccaa gacgttcccc cttcttatca aggctttgtg
      841 acggggatga tggctgttgg aacccggcac cgcaagggtt ttgagactat agtccttccg
      901 catagtgcag ggcctgagga caaaccccat tattggcgca tcagcttacg gaattggcac
      961 atgaccttac gattccccat tctccttctc ggcgtgcttg ccctgtttgc atggcagccg
     1021 gtggccacgg cagcgcagcg cgttgcggtc ttcgatttcg aactgatcga caccagcctc
     1081 gaaggcgaaa cctatggcac gcgcgccgac gagaaggagc ggctgaagaa gttgagcgat
     1141 cagctgcgcc aggcccttgc caagtctggg caatttgagg tcgtcgatat cgggcctgtc
     1201 gaagccgctg cgcaaggagc gaacttgcag gcttgcggtg gctgcgatgg aacgctcgcg
     1261 cagaaagtgg gcgccgatct ctcgatcacc ggcacagtgc aaaaaatatc gaacctgatc
     1321 ctgaatatga atatctacgt tcgtgatgtc tccagtcaga aacttatcgc gagcagaagc
     1381 gccgattttc ggagcaatac ggatgaatcc tggtctcgca ctctcaaata tatcgttaaa
     1441 aattatctac ttgcacccaa tttcggaagc aattaaagct tgctattctt gatattctgt
     1501 gcacggaaag acgagttccg tacacagaaa atatctttca agtctcaaag ttttgaacgt
     1561 cacagtgtac ggaacactat ccctttttga aatgagatcc ctttagataa agaccgcaaa
     1621 atgcgtcaat gccgcatgcg ctgttattgc ccgtctgcaa aatatgcagt ggctggcata
     1681 ctaattgctg agcttattca ggctcaacag aaaggaacaa acaggaatgc tggacctcgt
     1741 cttgacggtt tgcgcgatcg cacagccggc cacgtgccgg gatcaacacg tattaatcga
     1801 ttccgatacc acaccatggc aatgcgcaat gagcgccccg ccgttcatag caaaatgggg
     1861 ttccgagcat ccggattggc gtgtccagcg gtggacttgt caatatccta agaaggaaaa
     1921 ggatatctag taagccgggg tgcggacgac ctacgaagtg ttgagtattc tcctaacgga
     1981 gaaaggtgca ttgtaagatc acgcttagac gaacggcttc ccgtgtagaa ggaaacgagc
     2041 gtgacacttg ccaagatgcg ctttgccgca agcgtcgtgg gtctctgtgt gacggcactg
     2101 tggtacgacg ctgcagctca ggacatgctg cagtatctgg atttgaattc gcccgacatg
     2161 acgcgagccg agatgacgcg ctccgatgtc gaggccatga taaaagcggc gcagggcaac
     2221 gctcttgatc tgtccggcaa gcgcctttcc gggcttgacc tgtccggtct cgacctgacg
     2281 cgcgccaatc tcagctcgac caggctgaac aacacgagtt ttgcaaaagc caggctcgat
     2341 ggtgccctcc tggatttgtc ctgggcgctc aaggccaatt tttcgggcgc ttccctcaag
     2401 ggcgcatcct tgttttctgc ccagatgcag ggctctaaat tcgatcatgc cgatctttca
     2461 ggcgcacgca tcgcggcaaa tctcacaggc gcaagtttgc gcgacgccaa catcaccggc
     2521 gcaaatggca gcccggacat gaaaaaccag tccatgggtc ttatgcggac ggtgctgaaa
     2581 tcggccaatc tcgaaggtgc ggacctttca ggcagcaacc ttacgcgcgt cgatcttgcc
     2641 ttcgctaatt tgcagggagc cgacttatcg ggagcgaacc tccgc
//
LOCUS       DATMGX010000647         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_400679, whole genome shotgun sequence.
ACCESSION   DATMGX010000647 DATMGX010000000
VERSION     DATMGX010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400679"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VNR65_10570"
     CDS             <1..254
                     /locus_tag="VNR65_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19158.1"
                     /translation="LKRSGVALVTYVGASGTVLVRAAAILGVSFGIALLSRKYLENPF
                     LSLKDRFTEADALRPQAHGPKVASDPAGIETLADGVNSN"
     gene            280..1506
                     /locus_tag="VNR65_10575"
     CDS             280..1506
                     /locus_tag="VNR65_10575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ19159.1"
                     /translation="MRLAYLLSEYPTLGHTYLLREVRQLRELDWDIQTISIRRPGNRT
                     SPLSPAEVEEWRSTWYILGGDVLGLLMSHVVTFVTRPLRYLRGLATAWRFGRFNPRQT
                     FLATAYFVEAVGAGHRLRRAGINYVHSVYSTTVALILSRVFDVNLSMTFHGPAEFTDP
                     EGFAIREKVRAAQLVCSISYFGKSQIMLWSSSSDWQKLEVAPLGVEISGWQTADFREH
                     PAPFELVSVGRLVEIKGFPLLLDAVAQLRSEGREVRLTLAGDGPDRAGLESQARRLGI
                     ADRVVFAGWKNQDDLRQLYLASDVCVLSSFAEGVPVVLMEAMATGVPCVAPRINGIPE
                     LIRDGVDGLLFTASDVQELVAAIGKLMDNPGLRRCMAESSPRRVADKYNVQKNVRRLS
                     EIFKHWMRRQATSSGI"
     gene            complement(1463..>2272)
                     /locus_tag="VNR65_10580"
     CDS             complement(1463..>2272)
                     /locus_tag="VNR65_10580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ19160.1"
                     /translation="AGTPAVISSQRGHRDLTGDLNKRFLRITDRLTDAVVVNCFAMQR
                     YLVDEEGVPGSKIRICRNAVDVELYRRHAVASPWAGKSVIGVVCALRPEKGLDTLLRA
                     FAKLSRQDTVLAIVGSGPEAPGLKTLAAELGIIERCHFEPATSEVAEWLSRIDIFVLP
                     SRTEALSNSLMEAMACGCGVIASRVGGNPELVDDGENGLLFDVDNVEQLAEQMEALLA
                     RPERRQRLAAAASSKMSGGFTYSQAAATMQEIYDSLLLRSRTKWPAASSNV"
BASE COUNT          416 a          710 c          655 g          491 t
ORIGIN      
        1 tactaaagcg ctccggtgtt gccctcgtta cttacgtcgg cgcttctggc acggtgctcg
       61 tgcgtgctgc ggcaatcctg ggtgtatctt tcggtatcgc cctattgtcc cgaaagtacc
      121 ttgagaaccc gttcttgtcg ttaaaagata gattcaccga agcggacgct ctgagacctc
      181 aggctcatgg accgaaggtc gcgagcgatc ctgctggtat tgagactctc gctgacggag
      241 tgaactcaaa ctaacgctcc actgagaagc accctcctta tgagactggc ctatcttctg
      301 agcgaatatc caacgctggg gcacacgtac ctgctgcgcg aggtgcggca actacgcgaa
      361 cttgactggg acatccagac gatttcaatt cgccggccag gcaaccggac ttccccgctc
      421 tctcccgcag aagtcgagga atggcgttcc acctggtaca ttctcggcgg cgatgtactt
      481 ggccttctga tgagccatgt ggttacgttt gtaacccgtc ctttgcgcta tctccgcggt
      541 ctggcaaccg catggcggtt cggccgattc aacccccggc agacatttct ggcaacggct
      601 tacttcgtcg aagcggtggg tgccggccat cgactgcgcc gggctggcat caattacgtt
      661 cactcggttt actccacgac ggtcgccctg attctctcgc gcgtattcga cgtcaatctg
      721 tcaatgacct ttcatggtcc ggcggaattc accgatcccg aaggtttcgc cattcgcgag
      781 aaagtccggg ctgctcagtt ggtgtgctcc atcagttatt tcggaaaaag ccagatcatg
      841 ctctggtcgt cttcctccga ctggcagaag ttggaggtcg cgccgcttgg ggtcgaaatt
      901 tccggatggc agacggcgga tttccgcgag catcctgcac cctttgaatt ggtatccgtc
      961 gggcgcctgg tggagatcaa gggctttcct ctgctcctgg atgccgtcgc ccaattgcgc
     1021 agcgagggcc gcgaggttcg cctgaccctg gcgggagacg gcccggaccg cgccggcttg
     1081 gaatcccagg cccggcggtt ggggattgcc gatcgcgttg tcttcgccgg atggaagaac
     1141 caggatgatt tgcgccagct gtacttggcc agcgatgtct gtgtcctgtc cagcttcgcg
     1201 gagggcgttc ccgtggtgct gatggaagcc atggcgaccg gtgtgccctg cgtcgctccc
     1261 cggatcaacg gcattccaga gttgatacgc gacggagttg acggccttct tttcaccgcg
     1321 tcggatgttc aggaactagt cgccgctatc ggcaaactca tggacaaccc cggcttgcgc
     1381 cgttgcatgg ccgaatcgtc tccccggcgg gtcgctgaca agtataacgt tcagaagaat
     1441 gtgcggcgtc tgtcagagat tttcaaacat tggatgaggc ggcaggccac ttcgtccggg
     1501 atctgagcag caacgaatcg taaatctcct gcatcgtggc cgccgcctgc gaataggtga
     1561 agcctcccga catcttagat gacgcggctg ccgctaaccg ttgccggcgt tcgggacggg
     1621 ccagaagcgc ttccatctgc tcagccagtt gttcgacatt gtctacgtcg aacagcagtc
     1681 cgttttcgcc gtcatcgacg agttccggat tgcccccaac ccgcgacgcg ataactccac
     1741 atccgcacgc catcgcttcc atcagggagt tagagagggc ttcggtgcgc gacggcaaca
     1801 caaagatgtc gatccggctg agccactcgg ccacctcgga cgtcgctggt tcgaagtggc
     1861 accgctcgat gatgccaagc tccgcagcca gcgttttcaa acccggcgcc tccggcccgc
     1921 tccccacgat cgccagcacc gtgtcctgcc gtgacagctt agcgaacgcc cgcagtagcg
     1981 tatccagccc cttctctggt cgaagcgcgc acaccacgcc aattaccgac ttgcccgccc
     2041 atggcgacgc gaccgcgtgg cgccggtaca gttcgacatc caccgcgttg cggcagatgc
     2101 ggatcttaga ccctggcacg ccttcctcgt caaccaagta ccgttgcatg gcgaagcaat
     2161 tcaccaccac cgcatcggtc aggcgatcgg taattcgcag gaagcgcttg ttcaggtcgc
     2221 cggtcagatc acgatggccc cgctggctcg agatgacggc aggcgtgcca gc
//
LOCUS       DATMGX010000648         2225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_193145, whole genome shotgun sequence.
ACCESSION   DATMGX010000648 DATMGX010000000
VERSION     DATMGX010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193145"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..500)
                     /locus_tag="VNR65_10585"
     CDS             complement(60..500)
                     /locus_tag="VNR65_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19161.1"
                     /translation="MIAVIIIACGAASPSKPEDAAPTTSFRVFVDMKTFMEHVLGPAA
                     AIIWKVNAVVIDQNGEHDLSPKSDKDWEQFVSGAGTLAEATNALMIPQRVRDPSWIPY
                     VRKLADAADKAYRAAEAHDLKSISEVSDELDGICAACHHHYGLE"
     gene            complement(529..1035)
                     /locus_tag="VNR65_10590"
     CDS             complement(529..1035)
                     /locus_tag="VNR65_10590"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HWJ19162.1"
                     /translation="MMQQLRGFIAYFENSTWADSIRENDVLFPLIESVHVVAICLVIG
                     SILAVDLRLLGLASINRSVTRVSSGILPLTWAAFAVAVASGSLLFISNASKYLENGYF
                     VAKMCLIGAAGLNMAIFHLRSAKDLPRWENETSPPFKARLAGGLSILLWITVVTCGRW
                     IGFTMPIR"
     gene            complement(1032..1535)
                     /locus_tag="VNR65_10595"
     CDS             complement(1032..1535)
                     /locus_tag="VNR65_10595"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HWJ19163.1"
                     /translation="MDAELIAVCKWLAATSLSHAIQTAGWIIPTVQTVHILCVAVLLS
                     SAVLVDLRLLRLLGRDLPLPEIMRRYLPTIWPVLLILLITGSVLIIGEPRRSLVNATF
                     YLKMALLAVAILLTGGLQWSISTSSNFWDLNRTRRVAGRIAATVSILVWCGVLFAGRW
                     IAYTQAG"
     gene            complement(1543..1938)
                     /locus_tag="VNR65_10600"
     CDS             complement(1543..1938)
                     /locus_tag="VNR65_10600"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWJ19164.1"
                     /translation="MVSPKSVLIAVSLIMGLFGIAAAHHSNAAYDVDHPQTMQGTVKT
                     VNWTNPHISFVIEKDVKAGEPAETWVFEVSSPGVLTRSGWTKHSLQPGDHAIFHYAPL
                     RDGKPGGYLQKVTLPSGQELSYSLTPPGQ"
     gene            complement(1950..>2225)
                     /locus_tag="VNR65_10605"
     CDS             complement(1950..>2225)
                     /locus_tag="VNR65_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19165.1"
                     /translation="GIPNNGDMHILERLYLAKPNVLYDDLTITAPKVLATTWKTRRIF
                     QRYPERHYEITEGECVQEDLIAGKDEFGNDIYVPNPQGPYGSVHTLK"
BASE COUNT          504 a          633 c          618 g          470 t
ORIGIN      
        1 gcatctcctt gcgcccgtga cgccgagatt caaactataa attcccgccg acagttattt
       61 tattcgagcc cgtagtgatg gtgacaggcc gcgcaaattc catcgagttc gtcgctgact
      121 tccgagatgg atttcagatc gtgcgcctcc gcggcgcgat atgccttgtc ggctgcgtcc
      181 gccagtttcc tgacgtaggg gatccaactc ggatcgcgca ctctctgcgg aatcattaac
      241 gcgttggtgg cttcagccag tgttccggcg ccgctcacga attgctccca gtctttgtct
      301 gacttgggag acagatcatg ctcaccgttt tgatcgatga ccacggcatt tactttccag
      361 atgatcgctg cggccggacc aagaacatgc tccatgaagg tcttcatgtc gacaaaaacc
      421 cggaatgaag tcgtcggagc cgcgtcttcg ggtttcgacg gcgatgcggc gccgcacgcg
      481 atgatgatca ccgcgatcat cgtcggtaga aatcggatcc tggccatctc agcgaattgg
      541 catggtaaag ccgatccagc gcccgcaggt gaccaccgtg atccatagca ggatcgacaa
      601 tcctcccgcc aagcgcgcct tgaacggcgg cgacgtttcg ttctcccagc gcggcaggtc
      661 cttggcgctg cggagatgaa agattgccat gttcaggccg gcagcgccaa tcaggcacat
      721 cttggcgaca aaatagccat tctccaggta ctttgaagca ttcgaaatga aaagcaggct
      781 ccccgaagca acggcaactg cgaaggccgc ccaagtgagc ggcaaaatcc cgcttgaaac
      841 gcgggttact gaacgattga tggatgcgag accgagcaat ctcagatcaa ccgccaggat
      901 ggatccgatg accaggcata ttgcaaccac atgcaccgat tcaatgaggg gaaacaggac
      961 atcgttctca cgaatactgt ccgcccatgt gctgttttcg aaataggcaa tgaatccacg
     1021 aagctgctgc atcatccggc ctgcgtatac gcgatccagc gaccggcgaa tagaacgccg
     1081 caccagacca ggatcgaaac ggttgccgca attcggcccg ccacccgccg ggttcgattc
     1141 agatcccaga agttcgacga cgtggatatc gaccactgca ggccgcccgt cagtaaaatt
     1201 gcgacggcaa gcagcgccat cttgagatag aaggtcgcat tgaccagcga tcgccgcggt
     1261 tcgccgatga tcagcacgct tccggtgatt aagaggatca gcagcacagg ccagatagtc
     1321 ggaaggtaac gccgcatgat ttcaggcaac ggcaggtcac gcccgagcaa gcgcaacagc
     1381 cgcaagtcga ccagcacggc cgaagacaat agaaccgcca cgcaaagaat atggacggtc
     1441 tgcacggttg gaatgatcca gccggcggtc tgaatcgcat ggctcagcga ggtcgccgcc
     1501 agccacttgc aaaccgcgat gagctcggca tccatcttgc cgttattgcc ccggaggcgt
     1561 taaggaataa ctgagttcct gaccgctcgg tagcgtaact ttctgtaggt acccacctgg
     1621 cttgccgtcg cggagcggcg catagtggaa aatcgcgtga tcccccggct ggagcgagtg
     1681 tttggtccag ccagagcgtg ttaatactcc gggactcgat acttcgaaca cccaggtttc
     1741 tgccggctcg cccgctttga catccttctc gatgacgaag gagatgtgag ggttggtcca
     1801 gttgacggtc ttgacggtgc cttgcattgt ttgcggatga tcgacatcgt aagccgcatt
     1861 cgaatgatgt gcggccgcga tgccgaagag acccatgatg aggctcacgg caatcaaaac
     1921 actcttgggt gataccattt cgatcgcgct cacttcaacg tgtggaccga tccatacgga
     1981 ccttgtggat ttggaacgta gatgtcgttg ccgaattcat ccttcccggc gatgagatcc
     2041 tcctggacac attcgccttc agtgatctca tagtgccgtt cgggatagcg ctgaaaaatt
     2101 ctcctggtct tccaggtggt cgccagtact tttggcgccg tgattgtcag atcgtcataa
     2161 aggacattgg gcttcgcgag atagagccgc tcgagaatat gcatgtcgcc gttattcgga
     2221 attcc
//
LOCUS       DATMGX010000649         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_410602, whole genome shotgun sequence.
ACCESSION   DATMGX010000649 DATMGX010000000
VERSION     DATMGX010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410602"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VNR65_10610"
     CDS             complement(<1..256)
                     /locus_tag="VNR65_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19166.1"
                     /translation="MFKRITLAAFASTAILSLAALSPASAQHHPGAGGMHLGGSGMHH
                     PGGFGGFHPGGFHPGGFHPHHPRWWRWHWRHYPYSQVFVEP"
     gene            636..1424
                     /locus_tag="VNR65_10615"
     CDS             636..1424
                     /locus_tag="VNR65_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWJ19167.1"
                     /translation="MGSYPLQSGSWLQRIVNRFPSRIMIVTALVASLCACSVNKKSHT
                     VDETQALSPRVIKVGDPVPKGGGEYKLGSAYLAGGKWYVPVNDQSYDETGIASWYGDF
                     FHGRRTANGEIYDMNALTAAHPTLPMPTYATVTNLANDRTIVVRINDRGPYHDGRIID
                     LSHKAAELLGFFGRGSAAVRVRYLSPAPLSGDDTLEQSMLAKQPWAPRVVRDKQAHAA
                     PSPKVSFLSAATVTAVPPLVTKKGGSEKAQKDISGGWVTNAQAD"
     gene            1545..>2113
                     /locus_tag="VNR65_10620"
     CDS             1545..>2113
                     /locus_tag="VNR65_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWJ19168.1"
                     /translation="MRLTILFLLLLLCPHSAGAQSAGTFQTSAGEAILVDVDTGSVLF
                     EKNADKLFAPASMAKIMTLEVVFKKLKAGEISLDTEFPVSLRAWKTGGAPSRTTSMFV
                     PLNQTAKVGDLIQGITVQNGNDACIAVAEGLSGSEDGFAQAMTEEARALGLEKSTFGN
                     STGLPNPTNQMTARELAKLSVYVIKEYPN"
BASE COUNT          460 a          598 c          623 g          432 t
ORIGIN      
        1 ccggttcgac gaagacctgg ctatatgggt agtgacgcca atgccagcgc caccagcgcg
       61 ggtgatgcgg gtggaagcca ccagggtgga acccgccagg atggaagccg ccaaagccac
      121 cgggatgatg cattccgctc cctccaagat gcataccgcc cgccccagga tggtgctggg
      181 cggaggccgg ggagagcgct gctaacgaga gaatggcggt cgaggcaaaa gccgcgaggg
      241 tgatgcgctt gaacatggtc gtctcctttg attgggtgtt ctaagagcgg gtggcttcag
      301 ctttttctgc acgctcaccg tcatcccatt tgtcgaagcg accgttcgag aggttcacga
      361 accggttcaa aaaattttcg gggccaagcg ctggacgcaa accggcgtca aacgaaacct
      421 gctcttgtga ccgtgatgcc ctgacgaaag tctgcgctcc cgcaaaagtc cgttaaccgt
      481 cctcgggtaa acctgagcgc agcgtttggt tgtgttttgg gtgtatgttt accatgagta
      541 gagagggtga cttctcgggg ggcgcttggg cccaaaaccc cgacagaacc ggctcaaggc
      601 cgtggtctgt gccccgcatt tgcccgaatc ggagcttggg atcctacccg ctgcagagtg
      661 ggtcttggtt gcagcgcatc gtcaaccgct tcccaagcag gatcatgatc gttacggctc
      721 tcgttgcatc tctttgtgcc tgttcagtga acaagaaatc gcacaccgtc gacgaaaccc
      781 aggctttgag cccgagggtc atcaaggttg gcgatcccgt tcctaagggc ggcggagaat
      841 ataagcttgg cagcgcgtat ctcgctggcg gcaaatggta tgtgccggtc aatgaccagt
      901 cctacgacga aactgggatt gcatcctggt atggggattt ctttcacgga cggcgcaccg
      961 ccaacggtga aatctacgac atgaacgccc tgacggcggc tcaccccacc ttgccgatgc
     1021 caacctatgc gaccgtcaca aaccttgcga acgatcgaac gatcgtcgtg cggatcaacg
     1081 accgcgggcc ttatcacgat ggccgtatca tcgacctttc gcacaaggca gcggagcttc
     1141 tcggcttttt cggacgcggc tcggccgccg tgcgcgtccg ctatctctcg ccggcgcctc
     1201 taagcggcga cgatacgctt gagcaatcca tgctggcgaa acagccctgg gcgccgcgtg
     1261 tcgtgagaga caagcaagcc catgctgccc cttcacccaa ggtgagcttt ctttcggcag
     1321 caaccgtaac ggcggtcccg cctctcgtca cgaagaaagg cggcagcgaa aaagctcaga
     1381 aggacatttc cgggggctgg gtgacgaacg cgcaagccga ttaacaaatg gcgttgcttc
     1441 agaagtcgca cagtcagatt atttgaaggc tcgctcgccg aacatttacc aaatcggggc
     1501 tgcagttgct atataatgca agcgattcag cctggattga gccgatgcgc ctcaccatac
     1561 tttttttact ccttctttta tgtccgcact cagcaggcgc acagtctgct ggtacgtttc
     1621 agacttctgc cggggaagcg atcctggttg atgtcgatac ggggtcggtg ctcttcgaga
     1681 agaacgccga caagcttttt gcgccagcga gcatggcaaa gatcatgacg cttgaggttg
     1741 tgttcaaaaa gctcaaggcc ggggaaatct cgctcgatac ggagtttccg gtgagcttgc
     1801 gcgcctggaa aacgggcggc gctccctcgc gtaccacgtc gatgttcgtt cccctcaacc
     1861 agacggcgaa agtcggcgac ctgatccaag gcatcaccgt gcagaatggt aatgacgcct
     1921 gcatcgccgt tgccgaaggg ttgtcaggga gcgaagacgg gttcgcacaa gccatgaccg
     1981 aagaggcgcg agcgctcggg cttgagaaat cgacgttcgg gaattcaacc ggactgccaa
     2041 atcccacgaa ccagatgacg gcacgggaac tggcaaagct ctcagtttat gtgatcaagg
     2101 aatatcctaa cta
//
LOCUS       DATMGX010000650         2193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_322636, whole genome shotgun sequence.
ACCESSION   DATMGX010000650 DATMGX010000000
VERSION     DATMGX010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322636"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1028)
                     /locus_tag="VNR65_10625"
     CDS             complement(99..1028)
                     /locus_tag="VNR65_10625"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ19169.1"
                     /translation="MRNITLKHFRVLSAVTKMGSMAGAARRLNVTPPAITVQVQQLEQ
                     AVGLPLVERFADKTTPTEAGEIILRTAHRIEALLAECGGEIAQLKGIEGGTVAVGVVS
                     TAKYFAPKALAAFRQKHPGVDVRLSVGNRQETIRALRDLELDLVIMGRPPENVDVDAA
                     VLGEHPHIIVAPAGHPLAGAKLTIADLAKETFFVRESGSGTRLLMERLFEQADFDPVV
                     GMEIGSNETIKQAVIAGLGIAFISAHTVAVEIADGRLEMLNVAGLPMVREWHAVRLRD
                     KAVMPAGQALWKFLSTEGQHFLPRPVSSPKKSL"
     gene            1189..>2193
                     /locus_tag="VNR65_10630"
     CDS             1189..>2193
                     /locus_tag="VNR65_10630"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF006780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HWJ19170.1"
                     /translation="MGAYATLREHLAEWAGDDTQRRAIAETITNLAKACVEVAHIVAL
                     GPLAGDMASKRGDHSDGDTQKELDFLSNKIVTNALKSSPVAWMGSEEDEKAVLLNPDA
                     PLVVNIDPLDGSSNIDTNVSIGTIFSILPSKATSPLLQPGSNQLAAGYVIYGPQTGMV
                     LTLGQGTHKFWMDTRNGEFVLVKANVQVPSVTREYGVNSSNFRHWDDAIKAYVLDCTL
                     GEDGPRKEDFNTRWIGSLVADAFRILVRGGIYIYPADGRKGYTSGRLRLLYEANPIAM
                     IMEQAGGACTTGIQRMMDIEPLSLHQRVPLIFGSKTEVEEVVHYYRDPHTLGDHSPLF
                     S"
BASE COUNT          475 a          659 c          614 g          445 t
ORIGIN      
        1 agatcgggcg cggatacagt catcttgcct tcctcatgca tcatcatctc gcatgagagt
       61 aaacccatct caggccgtga gaactccggc ccttgctatc agagtgactt ctttggggat
      121 gagaccggcc gcggaagaaa gtgctgccct tcggtcgaga ggaacttcca cagcgcctgg
      181 ccggccggca tcactgcctt gtccctgagc cgcacggcat gccactcccg caccataggc
      241 agccccgcga cattgagcat ctccaatcgg ccatccgcga tttcgacagc taccgtgtgc
      301 gctgagatga acgcgatccc aagccccgca atcaccgcct gcttgatcgt ttcatttgag
      361 ccgatttcca tccccacaac cggatcaaag tcagcctgct cgaacaggcg ttccatcagg
      421 aggcgagtgc cggatcccga ctcgcgcacg aaaaacgtct ctttggcgag gtccgcgatc
      481 gtcagctttg cccccgccag cggatgcccg gctggcgcga cgatgatgtg aggatgttcg
      541 ccgagcaccg cggcatcaac atcgacattc tccggaggac gccccatgat aacgaggtca
      601 agttccagat cacgcaaagc gcggatggtt tcctgcctgt ttccgaccga tagccgcaca
      661 tccacgcccg gatgtttttg gcggaaggca gccaaggcct tgggcgcgaa atattttgcc
      721 gtgctgacga cgcccaccgc gaccgttcca ccctcgatgc ccttcagctg agcgatttcg
      781 cccccgcatt cggcaagcag cgcctcgatg cggtgcgccg tcctcaggat gatttcgcct
      841 gcttctgtcg gggtggtctt gtcagcgaag cgctccacga gaggcagccc cacggcctgc
      901 tcaagctgct gaacctgcac ggtaattgca gggggtgtga cattcagtcg tcgcgctgcg
      961 cctgccatcg agcccatttt cgtgacggca gagagaacac ggaaatgttt cagggttatg
     1021 tttctcacgg agccagaata tgccattaga aaaaattaat caaacggaaa agaaattaaa
     1081 atttactttc tgctggatgc tccccatagt gcaaaaagtt gtcggtcaca accgagcgat
     1141 gagataagaa tagcggtgtg tcaaagaccg gcaagggagg taaacggcat gggcgcttat
     1201 gccacgttgc gtgagcattt ggcggaatgg gcgggcgacg acacgcagcg tcgcgcgatc
     1261 gccgaaacta tcacaaatct agccaaggcc tgcgtcgagg ttgcgcacat cgttgcgctc
     1321 gggccgctcg caggcgacat ggcgtcaaag cgtggcgacc actccgacgg ggacacccaa
     1381 aaagagctgg atttcctcag taacaagatt gtgaccaatg cgctgaagtc ttccccggtg
     1441 gcctggatgg ggtcggagga agatgaaaag gccgtgctgc tcaatccgga cgcgccgctg
     1501 gtggtcaata tcgatcccct cgacggttcc tccaatatcg atacaaacgt ctcgatcggc
     1561 acgatttttt cgatcctgcc ctcgaaagct acctcgccgc ttcttcagcc gggcagtaac
     1621 cagcttgccg cagggtatgt catctatggt ccgcaaacgg gaatggtgct gacccttggc
     1681 caaggaacgc acaaattctg gatggacacc agaaatggtg aattcgtgct cgtgaaggcg
     1741 aacgttcagg ttccctccgt aacgcgcgaa tacggcgtaa actcctcgaa cttcaggcat
     1801 tgggacgacg ccataaaggc ctacgtcctc gattgcacgc tcggcgagga cggcccgcgc
     1861 aaagaggatt tcaacacgcg ctggatcgga tcgcttgttg cggacgcctt ccgcatcctg
     1921 gttcgcggcg gcatctacat ttatccggcc gacggccgga agggatatac gagcggccgc
     1981 ttgcgcctcc tgtatgaagc caacccgatc gcgatgatca tggaacaggc cggcggagct
     2041 tgcacgaccg gtatccagcg gatgatggac atcgaacctt tgtcgctcca tcagcgcgtt
     2101 cccctcatct tcggctcaaa gaccgaagtc gaggaagtgg tgcactacta tcgcgatcct
     2161 cacacgcttg gcgatcattc acctctgttt tcg
//
LOCUS       DATMGX010000651         2567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_15520, whole genome shotgun sequence.
ACCESSION   DATMGX010000651 DATMGX010000000
VERSION     DATMGX010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15520"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VNR65_10635"
     CDS             complement(<1..489)
                     /locus_tag="VNR65_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="with PdxT forms pyridoxal 5'-phosphate from
                     glutamine, either ribose 5-phosphate or ribulose
                     5-phosphate, and either glyceraldehyde 3-phosphate or
                     dihydroxyacetone phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HWJ19171.1"
                     /translation="MSENNGNNTSLRLKTGLAEMLKGGVIMDVMSVEQAEVAEKAGAT
                     AVMALERVPAIIRAEGGVSRMANPKLIRQIMATVSIPVMAKCRIGHFAEAQILQELGV
                     DYIDESEVLTPADEEHHVDKHAFKTPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGD
                     VVH"
     gene            complement(740..1225)
                     /locus_tag="VNR65_10640"
     CDS             complement(740..1225)
                     /locus_tag="VNR65_10640"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWJ19172.1"
                     /translation="MPRHEKIHGHEVKKQQYTNNCFGCGPANAAGLRLKFVLDKKRER
                     FLCRFRLQRRFVGPPAHAHGGIIATILDEAMGKVNKLHNLIALTSQMEVTYLKPVPLF
                     KPLIVEGWEVRVHGREHYRAAEIRNREGEVLASSTSKFIEVDAHRMFAKYLKKKHSDA
                     T"
     gene            complement(1400..1975)
                     /locus_tag="VNR65_10645"
     CDS             complement(1400..1975)
                     /locus_tag="VNR65_10645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ19173.1"
                     /translation="MEENARIEWDRRHAEGSHSSLTPDPFFVSAYEQFVAPNFKYVGR
                     ALDIAGGIGRHAIHLAERGWLVTLMDISDVALKQARRHAEERDVHILAEQIDLTEAQL
                     PASAFDLVLVFFYLERSLLPQIADALRPGGILIYKTYTREQLKMVGGPTHPMHLLEPN
                     ELQGAFSQLRILHYVETIEDKAVAELVARKE"
     gene            complement(2138..2440)
                     /locus_tag="VNR65_10650"
     CDS             complement(2138..2440)
                     /locus_tag="VNR65_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19174.1"
                     /translation="MKRIIIIVVVLAGILYLADFLSVRFRIPARDTFGTVTLHTYYVV
                     KLKNGRTEYDYAGDHNASCSNSVFPQFGVKPCWYASRHTEEQINIDSGTPNNPHLF"
BASE COUNT          518 a          751 c          730 g          568 t
ORIGIN      
        1 atggactaca tccccggttc cagcctcgcc tttggtgcgg atcatagcgg cgccttcggc
       61 aatgcgccgc agtgcttcgc ccaaattgcg cgcgccgcaa acgaacggag tcttgaacgc
      121 atgcttgtcc acatgatgct cttcgtcggc tggcgtcagc acttccgact cgtcgatata
      181 gtcaaccccg agttcctgca gaatctgcgc ctcggcgaag tggccgatgc ggcacttggc
      241 cattaccggg atcgacactg tggccatgat ctggcgaatc agcttgggat tggccatgcg
      301 cgagacaccg ccctcggcgc ggatgatggc gggcacgcgc tccagcgcca tgaccgcggt
      361 tgcgccggcc ttctcggcga cctcggcctg ttcgacgctc atcacgtcca tgatgacgcc
      421 gcccttgagc atctcggcca aaccggtctt caggcgcagg ctggtgttgt tgccattgtt
      481 ctctgacata aacttctccc cggatcgcag catttagcac ttggcagttg gcatttggtg
      541 ttggtcgtga agctttggcc aaatgctaac cgctaactgc ctagcagcag cgcgatcctg
      601 tatctcgcgg aaccctttat tttactctgc gcggacgcgc cgcgtaaccc cgtgccatgg
      661 aacgcggacc cgaaggtccg cactacccga acgtcgcttt tttcggcagc ttctaaagcc
      721 gagtggcccg ccgttcgcgc tatgtcgcgt cgctgtgctt cttcttcagg tacttggcga
      781 acatgcgatg cgcatcgacc tcgatgaact tcgacgtact gctcgccagc acctctcctt
      841 ctcgattgcg aatctcggcg gcccggtaat gttcgcggcc gtggacgcgc acctcccatc
      901 cttcgacaat gagcggtttg aacagcggta ccggcttcag gtaggtgact tccatctggc
      961 tcgtcagggc aatcagattg tgcagcttgt tcaccttgcc catggcttcg tcgaggatgg
     1021 tggcgatgat gcctccgtgg gcatgggctg gcggcccgac aaagcgccgt tgcaggcgaa
     1081 agcggcagag gaagcgctcg cgtttcttgt cgagaacaaa cttcagccgg agtccggctg
     1141 cgttggcggg accgcaaccg aagcaattgt tggtgtactg ctgtttcttt acctcgtgac
     1201 catgaatttt ctcgtgccgg ggcatgtaca caagatgaag cagactcgat caggatgcaa
     1261 gtgcgaagac gcttttcgct tttcgccatt cgctatttgc tgaggagatg gcacagtgtc
     1321 gccatggact gtcttgcgaa gagcgaaaag cgaagagcca aatgcgaaaa gcgaacagcg
     1381 aagggcggcc agccgacagc tattccttcc gcgccacaag ctcggcaacc gccttgtcct
     1441 cgatcgtctc aacgtagtgc agtatccgta gctgggaaaa cgcgccctgc aattcgttcg
     1501 gctccagcaa gtgcatagga tgtgtcggac cgccgaccat cttgagctgt tcccgggtgt
     1561 aagtcttgta gatgagaatg ccgccgggcc tgagcgcgtc cgcgatctgt ggcaacagcg
     1621 agcgctcgag atagaagaaa accaggacca gatcgaaggc tgacgcgggc agttgggctt
     1681 ccgtcagatc gatctgctcg gcaagaatat gaacgtcccg ctcctcggcg tgtcgcctgg
     1741 cttgtttcaa ggccacgtcc gaaatatcca tcagcgtcac cagccaaccg cgttcggcca
     1801 gatgaatcgc atgacggcct atgccgccgg cgatatcgag cgctcgcccc acatacttaa
     1861 agtttggcgc gacgaactgc tcgtaagcgc tgacaaagaa aggatcgggc gtaagcgagc
     1921 tgtgagagcc ctcggcgtgg cggcggtccc attcgatgcg ggcattctct tccatagttt
     1981 tctggagctg tcagatctca gctgtcagct atcggttgtc agcgatctca acagacgctt
     2041 ttcgcttttt gctcttcgct tttcgcaaga cagtccgtgt cgacaccagg ccatcacctt
     2101 agcgaagagc gagaagcgaa tagcgaaaag cgactcctca aaacagatgc ggattattcg
     2161 gggtgccaga gtcgatgttg atctgttcct cggtgtgccg gctggcgtac cagcagggct
     2221 ttaccccgaa ctgcggaaac acggagttcg agcaacttgc gttatggtcg cccgcgtagt
     2281 cgtactcggt ccttccattc ttcaacttga cgacgtagta ggtatgcaat gtcacggtgc
     2341 caaaggtgtc gcgggccggt atgcgaaagc ggaccgagag aaagtcggcc agatacagaa
     2401 tacccgctag aacaaccacg atgatgataa tgcgcttcat atgccggaca gcgattcgcc
     2461 gccgtattgt aagtcagcaa ccagttgtag gttcggggat cgccccagct ttcggccggg
     2521 caaaatctct gcccgccaaa atattccttg tctccaaaat atacctc
//
LOCUS       DATMGX010000652         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_330207, whole genome shotgun sequence.
ACCESSION   DATMGX010000652 DATMGX010000000
VERSION     DATMGX010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330207"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VNR65_10655"
     CDS             complement(<1..216)
                     /locus_tag="VNR65_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19175.1"
                     /translation="MRQTIPTATGERLFFKRLVIGVISFLTLVDLFAAQAILPSLAAA
                     YGVSPAAMAFAVNASTFGMAAAGIAVAL"
     gene            complement(396..1901)
                     /locus_tag="VNR65_10660"
     CDS             complement(396..1901)
                     /locus_tag="VNR65_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ19176.1"
                     /translation="MAEGLVERYDDRIAGVLSCYDRIVITGTLPGVCHARGMTSFLYS
                     NAIRIFDYPQFAATLRDTVRENAAKVAEAAGVAIEHISKSHIRKEDIVAKAIARRGEH
                     PGLAHVISAMEACGAYEPWHDKKTHKTFLRPDSGKCLHYYFYFIDEELGLIYVRVPTW
                     APFRLQVYCNGHSWLARKLTAEGIGFTAGDNAFLRIGDWERAQELADGFSPGMLHRIL
                     DRYAALCCPVLDTFKQSYHWSLMQVEYSTDLVFHSKPTLAPLYEQLARHSVLTVKAET
                     IATFLGRSIAPNLAQEIGSQFSTRIEGTCIKHRFGKSSIKMYDKLGIVLRIETTTNDV
                     SSFKHYRKVEHRNGPSSMGFAPMKKTVYSLGDLAGLLFACNRRYLAHLSALDDFSAGV
                     RLLDRITRPRKVEGKTVKGLDFFNPTERTVLGALQNPKFNIAGVRRADLAPLLKKISP
                     ATLSRQIRRLRYLGLIKRAAKTYRYYLTCAGRAVIAAASHITEYGIVPALA"
BASE COUNT          412 a          551 c          676 g          400 t
ORIGIN      
        1 aagcgccaca gcgatgcctg cggcggccat gccaaaggtg ctggcgttga cggcaaacgc
       61 catggcggcc gggctgaccc catacgctgc cgcgagagac ggcaggatgg cctgtgctgc
      121 gaacaggtcc acgagcgtaa ggaagctgat gacgccaatg accagccgct tgaagaagag
      181 gcgttcgccg gttgcggtgg ggatcgtttg tctcatgttt gcgctgccag ggttgaaagg
      241 cgaccgcttt gaggatgaca gcgcgcggcg aaaggcggaa atgccgattg gctccaaaga
      301 caatgcctgc tggctatacc ggcttaacgc aacggttgta gctcagagtt tagtccctta
      361 ccaaccaaat cttgtcactt tgcgcacgga atgcgtcagg cgagtgcggg aacgatgccg
      421 tattcagtga tgtgagaggc ggcagcgatg acggcgcgtc cggcgcaggt gaggtagtag
      481 cggtaggttt tggcggcgcg tttgatgaga ccgagataac gcaatcggcg aatttggcgt
      541 gagagcgtgg cgggggagat tttcttgagc aatggcgcga gatcggcgcg gcggacacca
      601 gcgatattga acttggggtt ctgcaaggcg ccaagcacgg tgcgttcggt ggggttgaag
      661 aaatcgaggc ccttgacggt cttgccctca accttccgcg gccgcgtgat acggtccagc
      721 agtctgacgc cagctgagaa atcgtcgagc gccgagagat gggcgagata acggcggttg
      781 caggcaaaga gaaggcctgc caggtctcca aggctgtaga cggtcttttt catgggagca
      841 aagcccatgc tcgatgggcc gttgcgatgt tctaccttcc ggtagtgctt gaaggacgag
      901 acatcgttcg ttgtcgtctc gatacgcaaa acgatgccga gtttatcgta catcttgatg
      961 gaggacttgc cgaaccggtg cttgatgcag gttccctcga tccgggtgga gaactggctg
     1021 ccgatctcct gcgccagatt gggggcgatg ctgcggccga ggaatgtggc gatggtctca
     1081 gccttcacgg tgagcactga atgccgcgca agttgttcgt aaagcggcgc cagcgtcggc
     1141 ttggagtgga agaccagatc ggttgaatac tcaacctgca tcaggctcca gtgataggat
     1201 tgtttgaagg tgtcgagcac cgggcagcac agggccgcgt agcggtcgag gatgcggtgg
     1261 agcataccgg gcgagaagcc gtcggccaat tcctgggcgc gctcccaatc gccgatgcgc
     1321 aggaatgcgt tgtccccggc cgtgaagccg atgccttcgg cggtcagctt gcgcgccagc
     1381 cagctgtggc cgttgcaata gacctgcaaa cggaacggcg cccaggtcgg aacgcggaca
     1441 tagatcaggc caagctcctc atcgatgaaa tagaaataat agtgcaggca tttgccgcta
     1501 tccgggcgca ggaaggtctt gtgagttttc ttgtcgtgcc acggctcgta ggcgccgcag
     1561 gcctccatgg cggagatgac gtgcgcgaga ccggggtgct cgccgcgccg ggcgatcgcc
     1621 ttggcgacaa tgtcctcctt gcggatgtgg ctcttcgaaa tatgctcgat ggcgacgccc
     1681 gcggcttccg caaccttcgc cgcgttctcc cggaccgtat cgcgcagcgt tgccgcaaac
     1741 tgcggataat cgaaaatgcg gatggcgttc gaataaagaa acgatgtcat gcccctggcg
     1801 tggcagaccc caggaagcgt gccggtgatg acgatccggt cgtaacaaga aagaacccct
     1861 gcgatccgat cgtcgtagcg ttcaacaagc ccctccgcca ttgtcccctc gccaagtggc
     1921 tgcgcccgcc accactatag cgtaacctct ttggttccgg ctccgccggc ttaggtattg
     1981 gggaaaatca ggcttcctgg ccgccaaggt tgcggcgaaa ggctttttcg agggcaacg
//
LOCUS       DATMGX010000653         2842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_256869, whole genome shotgun sequence.
ACCESSION   DATMGX010000653 DATMGX010000000
VERSION     DATMGX010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256869"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..2104)
                     /locus_tag="VNR65_10665"
     CDS             complement(209..2104)
                     /locus_tag="VNR65_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ19177.1"
                     /translation="MLSWFEKLIDPFKEVPVEQPPSKIGAFYWHFLRPVWWVYALIML
                     FGLIGALIEVSLFAFVGKIVDLAKTAKDPASFFEEHGTTLIWMAFVAMVARPVAFAVH
                     ALLINQTANANVMSKVRWQQHRYMLRQSLAFFQNDFAGRLANKIMQTGGALRQTVVEV
                     VDALWFVAIYWTSAAVIFFDLDWRLVIPLVLWLLTFLGMIWFFVPRLMRYATIVSDAR
                     SMLTGRIVDSYTNILTVKLFAHTEGEDAYAKEAIADHLNKLKKQLRWITGMELTQWVL
                     TGMLITGTTGLALWLWTQGAITLGAIAVITGLVIRINNMAGWIMWVVTGIFENLGTVQ
                     EGMEAISKPWTVVDAPDAKPLRVSQGEIRYEGVRFHYGRDSGLIENLSLTIRPGEKVG
                     LVGPSGAGKSTLINILLRFHDLEAGRILVDGQNIAAVGQDSLRAQIGLVTQDTSLLHR
                     SVMDNIRYGRPGATEDDVIAAAKQSHAHEFILDLEDYKGRRGYKAQVGERGVKLSGGQ
                     RQRVAIARVLLKNAPILILDEATSALDSEVEAAIQEQLNNLMKNKTVIAIAHRLSTIA
                     AMDRLIIMEKGSIVEDGSHEELLRHGGLYASLWARQSGGFLFEDLKPTDPMLHPDRMA
                     EIEGFAE"
BASE COUNT          611 a          832 c          780 g          619 t
ORIGIN      
        1 atggctggaa ttgcggcggt ccgtcttgtg ccccttccta cgccgaccgt atggcggcgt
       61 gtgcagccaa ctttgtgtcc ttcgatccgt ttaccgggac ctacacgacc tatgaaggct
      121 tccaggttct ttgccccttc cttcgttaag tcgactgtgt cctgaggcgg cctagcggtt
      181 ccaggcgcag ctatgtgact ggaaccgcct actcggcgaa gccctctatt tcagccattc
      241 gatccgggtg cagcatcgga tcggtcggtt tgagatcctc gaacaggaag ccacccgatt
      301 gccgcgccca aaggctcgcg taaagcccgc catggcgaag cagctcctcg tggctgccgt
      361 cctcgacgat gctgcccttc tccatgatga tgagccggtc catcgccgcg atcgtggaaa
      421 gccggtgcgc gatcgcgatc acggttttgt ttttcatcag attgtttagc tgctcttgga
      481 tcgcagcctc gacctcggaa tcgagcgctg atgtggcctc gtccaagatc aagattggcg
      541 cattcttcaa gagcacgcgc gcaatggcaa cgcgttggcg ctgcccgccg gaaagcttca
      601 cccctcgctc gccgacttgt gccttgtagc cgcgcctgcc tttgtaatcc tcgagatcga
      661 gaatgaactc atgggcatgg gattgtttcg cggcggcaat cacatcgtct tccgtagccc
      721 ctggcctgcc gtaacggatg ttgtccatga cggaccggtg caaaagcgac gtatcctgcg
      781 tcacaaggcc gatctgcgcg cgcaagctgt cctggccgac cgcggcaata ttctgcccgt
      841 cgaccaaaat gcgccctgcc tccaaatcgt gaaagcgcag gaggatattg atcagcgtgg
      901 acttgcccgc gcctgaaggc cccacgaggc cgactttttc gccgggcctg atcgtgagcg
      961 acaggttctc gatgagcccg ctgtcgcgcc cgtagtggaa gcgtacaccc tcgtagcgga
     1021 tttcgccctg cgagacgcgc aaaggcttcg cgtcaggagc gtccaccacc gtccaaggct
     1081 tcgaaatcgc ctccatgccc tcctgcacgg tgccgagatt ctcgaaaatg ccggttacga
     1141 cccacatgat ccatccggcc atattgttga tgcggatcac aagcccggtt atcactgcaa
     1201 tcgcgccgag cgtgatggct ccctgcgtcc aaagccaaag cgccagccct gtcgttccag
     1261 tgatgagcat acccgtcagc acccactggg tcagttccat cccggtgatc cagcgcagtt
     1321 gctttttcag cttgttcaga tggtctgcga tcgcctcctt cgcataggcg tcctcaccct
     1381 ccgtgtgagc aaacagcttt acggtcagga tgttggtgta gctgtctacg atgcggccgg
     1441 tcagcatgga gcgcgcgtcc gagacaatgg ttgcgtaacg catcagccgc ggcacgaaga
     1501 accagatcat gcccaggaaa gtcaggagcc acaaaacaag tgggattacg agccgccagt
     1561 cgaggtcgaa gaagatgacg gcggcgctcg tccaatagat cgcgacgaac caaagcgcgt
     1621 cgacgacctc gacgacagtt tgccgaagcg cgccgccggt ttgcatgatc ttgttggcga
     1681 ggcggcccgc gaaatcgttc tggaaaaagg cgaggctttg gcgcagcata tagcggtgct
     1741 gctgccagcg caccttgctc atgacgttag cgttcgccgt ttggttgatc aggagcgcgt
     1801 gcaccgcaaa ggcgacgggc cttgcgacca tcgcgacaaa cgccatccag atcagcgtgg
     1861 tcccgtgctc ctcgaagaac gaggcagggt ctttcgcagt cttggccaga tcgacgatct
     1921 ttccgacaaa agcgaagagc gaaacttcga tcagcgcgcc gatcagcccg aaaagcatga
     1981 tgagcgcata cacccaccag actgggcgca agaaatgcca gtagaaagcc ccgatcttgc
     2041 tcggcggctg ctccacgggt acttctttga aagggtcaat aagtttttcg aaccaggaaa
     2101 gcatggcttc gctcgcatga attgagattt agcaaatggc gcggcgaagc gaagctgacg
     2161 acctcgcgat agtgctcgcg cttaattacc ggacaatggc aattgtcatc ggcgagggcc
     2221 tcctgccgtt ttggaacttg gggaacaggg cttgtaccac gacagccaca gttggggaag
     2281 agtcatatca aaaaatgacc cgcgatccgc tctatctaca actttcggcc cagctcgttt
     2341 ctggagccac gttctcgcct ttatctataa ctacccacgt cccctccctg atcgttacgc
     2401 ttggatgtgg gtcaaagagt agcaaaacct tgggttgtaa accggttaag gggcagttta
     2461 tatcattgag aagcttatct cttttttcac gcttttgcta agagcaagac ctcagccgtg
     2521 aaaaaggtgg tatggtaaca aatcctcgag gaggggattg gcctgtggcg ttggcgaaaa
     2581 acttacgcta tcttgaaagt ttattccgca atctgttgct taaaaaacgc agattgttga
     2641 gtagccacat gctaacctta aagccgagtc acctatagaa actaaaccgc gttgaagcaa
     2701 agacgaaagt ctcatgctag ctataactgt tcttccgctt gagcaaactc cattccttcg
     2761 ggtacccagc gtcggctgca ccgtggaact gagacgctcg ccgcgcgcca aacgattttc
     2821 cctcaaggtt agccacactg ag
//
LOCUS       DATMGX010000654         4181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_300796, whole genome shotgun sequence.
ACCESSION   DATMGX010000654 DATMGX010000000
VERSION     DATMGX010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300796"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /gene="dxs"
                     /locus_tag="VNR65_10670"
     CDS             <1..1310
                     /gene="dxs"
                     /locus_tag="VNR65_10670"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HWJ19178.1"
                     /translation="KVRDIAKYFAHKLPKVWERRAARAEEFTRHLFTGGVLFEELGLY
                     YIGPIDGHNFDHLLPVLRNVRDVHEGPVLIHVVTQKGKGYEPAENSPDKYHGVTKFNV
                     VTGAQDKGKGGPPSYTKVFAQSLIEEARKDNKIVAVTAAMPAGTGLDLFEKEFPDRCF
                     DVGIAEQHAVTFCAGMATEGLKPFAAIYSTFLQRGYDQLVHDVALQHLPVRFVLDRGG
                     LVGADGATHAGSFDLAYLCCLPDFVVMAPADEVELKNMVATAAAIDNGPSAIRYPRGE
                     GFGYEMPAEGSVLPIGKGRIIREGSTVAILSLGTRLSESVKAADKLAAMGLSTTVADA
                     RFAKPLDYDLIRQLARHHEVLITVEEGSGGGFGSHVLEFIARDGLLDHGLKVRTLVLP
                     DVFMDHGKPEVMYENAGLNASGIIKAVVAALGTSGAVSRDGRA"
     gene            complement(1411..2046)
                     /locus_tag="VNR65_10675"
     CDS             complement(1411..2046)
                     /locus_tag="VNR65_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWJ19179.1"
                     /translation="MMLPRAIFWIAGGVAAAVVAGIAAGPVLSRVEQPDYKVEKQDGS
                     IEIRSYGPMIAAEAQVQGERKSAINEGFRLIAGYIFGKNEPQAKIEMTTPVEQQKSQK
                     IAMTSPVTQQGQENSWTVRFIMPKSWTMQTLPAPSDPRVKLEPLPARRFLVVTFSGLA
                     RDEAIESRTAELRQYAASNKLATVGEPLLAFYNPPWTLPMLRRNEIMLELA"
     gene            2171..2497
                     /locus_tag="VNR65_10680"
     CDS             2171..2497
                     /locus_tag="VNR65_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19180.1"
                     /translation="MRVRLHGELVEPWAASSLSVRPFRHCFANLVILDGARSKPDGKQ
                     FVATLSFNTKVVNRSAPDLLAVTDMINKQFIPAAITSRQAHFPAGSKSDRTLKSNSYL
                     PSMTSH"
     gene            2897..3325
                     /locus_tag="VNR65_10685"
     CDS             2897..3325
                     /locus_tag="VNR65_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWJ19181.1"
                     /translation="MKILRTRIEFETVEPIQILDITGQVRDWLKTTGVRDGLLTVTSL
                     HTTARVNVNEREPQLQKDMVTWLKRFVPCDGDWLHNLAAVDGRDNAHSHILGLFMNSS
                     ECIPVAGGEMILGGWQSIFFIELDGPRERRGLELQIMGEA"
     gene            3329..>4181
                     /locus_tag="VNR65_10690"
     CDS             3329..>4181
                     /locus_tag="VNR65_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HWJ19182.1"
                     /translation="MTPDDIAGFYAKRQDLNPEDYIELDFTFECAGDPREAAAHLCSE
                     QSTAQWRRVGVDEDFRPRFGAKVLDLESEPRPSGFSIPVECAVQGPVHAVRVTMAHPH
                     GNFGPRIPNLLSAVCGEGVFFSPGIPLIRLQDIRFPDTYLAAFEGPRFGVAGLREHLQ
                     AFDRPIFFGVIKPNIGLAAEPFAELGYESWLGGLDIAKDDEMLADVGWCPLTERAAAL
                     GEARRRAEHETGVPKIYLANITDEVDRLASLHDIAVAKGANALLVNAMPVGLSGMRML
                     RKHATVPL"
BASE COUNT          813 a         1223 c         1259 g          886 t
ORIGIN      
        1 tcaaggttcg cgatatcgcg aagtatttcg cgcacaagct tcccaaggtg tgggaacggc
       61 gggctgcgcg cgccgaggag ttcacccgcc atctgttcac gggcggcgtg ctgttcgagg
      121 aactcgggct ctattatatc ggccccatcg acgggcacaa tttcgatcat ctcttgcccg
      181 ttctgcgcaa tgttcgcgac gtccatgagg ggccggttct gatccacgtc gtaacccaga
      241 agggcaaagg ctacgaacct gccgagaatt cgcccgacaa gtatcacggt gtgaccaagt
      301 tcaacgtcgt caccggcgcc caggacaagg gcaagggcgg cccgccgagt tacacgaagg
      361 ttttcgcgca aagccttatc gaagaagcgc gcaaggacaa taagatcgtg gctgtcacgg
      421 cagctatgcc tgccggaact ggactcgatc tcttcgagaa ggagttcccc gaccgatgtt
      481 tcgacgtcgg catcgcggag cagcatgcgg tgaccttctg tgccggtatg gcaaccgagg
      541 ggctcaaacc ctttgccgcc atctattcaa ccttcctgca gcgcggctac gatcagcttg
      601 tgcatgacgt ggccttgcag catttgccgg tccgctttgt gctcgaccgc ggcgggcttg
      661 tgggcgcgga cggcgccact catgcaggtt ccttcgacct cgcctatctt tgctgcctgc
      721 cggatttcgt ggtgatggct cccgccgacg aggttgagtt gaagaacatg gtggcgaccg
      781 ctgccgctat cgacaatggg ccaagcgcga tccgttatcc gcgcggcgag ggatttggct
      841 acgaaatgcc ggctgagggc agcgtgctcc ctatcggcaa aggccgcatc atacgcgaag
      901 gctcaacggt cgcgatcctc tcgcttggca cgcgcctgtc cgagagtgtg aaggccgcag
      961 acaaactcgc cgcaatgggg ttgtcgacga cggttgcgga cgctcgcttc gccaaacctc
     1021 tcgattacga tctcattcgc cagttggcgc gccatcatga ggtgctgatc actgtagagg
     1081 aaggctcagg cggtggcttt ggctcgcatg tgcttgaatt tatcgcgcgc gatgggctcc
     1141 tggatcacgg cctgaaggtg cgtacgctcg tgcttcccga tgtgttcatg gatcacggca
     1201 agccggaagt catgtatgaa aacgccgggt tgaacgcgag cggcatcatt aaggccgtgg
     1261 ttgcggcgct tggcacatca ggagccgtgt cgcgggacgg gcgcgcgtag ccgcccgtgt
     1321 catggccgca cttgatgcgg ccatccattg aagcttagtc gatcggacgg caagcccggc
     1381 caagaccatt tccagccaag cgatgttgct ttaagcaagt tcgagcatga tctcgttgcg
     1441 gcggagcatt ggaagcgtcc agggcggatt gtagaaggca agcagcggct cgccgacggt
     1501 ggcgagcttg ttcgaagctg cgtactgccg caactctgcg gttctactct cgatggcctc
     1561 gtcgcgcgct aagccggaaa acgtcacaac aagaaagcgc cgcgccggca agggctccag
     1621 ttttacacgc gggtccgaag gggcaggcaa cgtctgcatc gtccatgatt tcggcatgat
     1681 gaaccgcacg gtccaggaat tctcctggcc ttgctgcgtg accggtgagg tcatcgcaat
     1741 cttctggctc ttctgctgct cgaccggggt ggtcatctcg atcttggcct gcggctcgtt
     1801 cttcccaaag atgtagcccg cgatcagccg gaatccttcg ttgatcgcgg acttgcgctc
     1861 gccctggact tgcgcctcag ccgcgatcat cggcccatag gagcgaattt cgatcgaacc
     1921 gtcctgtttt tcgaccttgt agtcgggctg ctcaacgcgg ctcaaaaccg gtcctgccgc
     1981 aatgccagca acgacggcgg cggcgactcc gccagcgatc cagaaaatag cgcggggtag
     2041 catcatgcat tcggttctcc aacgggtcgc tcatgcgtca cagatgttgt ttttcgcttc
     2101 cttccgcaag gcgggcagca ggctgctcag gacacgcaag gatcacacct gcgcgaagtc
     2161 ttagcgcggc gtgcgcgttc gacttcatgg cgaacttgtc gagccatggg ctgcatcgtc
     2221 tttgagcgtc cgccctttta ggcattgctt cgccaatctg gtcatcctgg acggcgctcg
     2281 cagcaagcct gatggtaaac aattcgtcgc aacgctgagc tttaatacaa aggtggttaa
     2341 caggagtgcg ccggatttac ttgcggtcac cgatatgatt aacaagcaat ttattcctgc
     2401 cgcaattact tcccgccaag cccacttccc ggccggaagt aaatccgata gaacattgaa
     2461 atcaaatagc tatctaccgt ccatgacctc ccattaaata gaattaaatg ctggaatttt
     2521 ctcatttact gggacgagca aaaatccgtt aaatatgttt cggcttcgtg aattaacgct
     2581 cgcgcacagc cgggacagct gcgggacagg cggcgcgacg ccgtcccgtt cggtattttt
     2641 cctcatattt atcagggttt ggagcgggct cacgctctcg ttaacctggc ggcaaagcga
     2701 tatcgccgat gatctttcat aggaaggaag acggacgaaa gcgtaagggg aaagttgaca
     2761 cacggatgcc gataattgcg agttgcatac aaaaaccgca cgcccgcgtc cgattacgcg
     2821 cacgatgctg tagcgtttgc ctcgacgagg ctcgcgcgat aacatccgtt tcgcccctcc
     2881 gcctcctagg tgccccatga aaatcctaag aacgcggatc gagttcgaga ccgtcgaacc
     2941 gatccagatc ctcgacatta caggacaggt ccgcgattgg ttgaaaacga ccggggtccg
     3001 cgatggcctt ctgacagtga catccttgca tacgaccgcg cgcgtgaatg tgaacgagcg
     3061 cgaaccgcaa ttgcagaagg atatggtgac ctggctgaag cggttcgtac cctgcgacgg
     3121 ggactggctg cacaatctgg ccgccgtgga cggacgcgac aacgcgcatt ctcacatcct
     3181 cgggcttttc atgaattcat ccgaatgcat tccggtcgcg ggcggcgaga tgatcctggg
     3241 cggttggcag tcgattttct ttatcgaact cgatgggccg cgggagcggc gcgggctcga
     3301 gcttcagatc atgggcgagg cttgattcat gacgccggac gacattgcgg gcttctacgc
     3361 caagcggcag gatctcaatc ccgaagacta catcgaactc gacttcacct tcgaatgcgc
     3421 tggtgacccg cgcgaggcag ccgcgcatct ctgcagcgag cagtccacgg cgcaatggcg
     3481 gcgcgtgggc gtggacgaag acttccgtcc gcgcttcggc gcgaaggtgc tggacttgga
     3541 atccgagcca cgtccttccg gttttagcat tccggtggaa tgcgcggtgc aggggccggt
     3601 gcatgcggtg cgcgtcacga tggcccatcc gcatggtaat ttcgggccgc gcattcccaa
     3661 tctgctctct gctgtctgcg gggagggcgt tttcttctct cccggcattc ccttgatccg
     3721 gcttcaggac atccgcttcc cggacactta tctggcggca ttcgaggggc cgagatttgg
     3781 tgtcgcgggc ttgcgcgagc acctgcaagc ctttgatcga ccaatcttct tcggcgtcat
     3841 caagcccaat atcgggctgg ctgccgagcc attcgccgaa ctgggctatg agagctggct
     3901 tggcggcctc gacatcgcca aggacgatga gatgctcgcc gatgtcggct ggtgcccgct
     3961 caccgagcgc gccgccgcgt tgggtgaagc gcgccgccgc gccgagcacg agaccggtgt
     4021 gcccaagatt tatctggcca acatcaccga cgaagttgac cggttggcca gccttcatga
     4081 catcgcggtc gcgaaaggcg cgaatgcgct tctggtcaac gccatgcctg tggggttgag
     4141 tggcatgcgg atgctgcgta agcacgcgac tgtgccgctg a
//
LOCUS       DATMGX010000655         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_169918, whole genome shotgun sequence.
ACCESSION   DATMGX010000655 DATMGX010000000
VERSION     DATMGX010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169918"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2066
                     /locus_tag="VNR65_10695"
     CDS             <1..>2066
                     /locus_tag="VNR65_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWJ19183.1"
                     /translation="GVTVTQYAFQSGEVGGTGEEAGQFKLRVMALEASGASVWEWDAR
                     RNEINTGSEVEHALGFSPGTLRCAADSWLQHLHASDRERMRLTLWTLRERQGGDINQD
                     FRMKRADGGYLWFELRAAVTQQRATRTLRGVGLLRNVNAQKRAQERLFHNAIRDGLTG
                     LPNRELFLDRLETAVARAKNEQTKPTVFFIDLDALKNQRSPDFTTNDGVLLTIARRLT
                     RHISPQDTLARVSALQFAILISEDTEPRHIAMLAERVRRSLRTPMKVGGRDLVLTGSI
                     GIAMYDGTQEHGEDLLREGESAMYRAKRAGSDRIELFKPEMRGAADERSIVQSDLKHA
                     IERRQIRVLYQPVMRIADERLAGMEAFVLWEHPTLGRLSLPEFEAAAESAGLSGELWA
                     YVFERCIRQGARWHRILQREDPIYISINMSSQQLFHNDLVQNLRLIIGRETLPKGALR
                     LEIAETLINSNPEQAIEILDWLKSLNVSVALDEFGVSFSSLSYWHRLSIDAIKIDRSL
                     VAMSDKERSSAMVLKAVLTIAHELAKDVIAVGVDNEEDLAYVRALGCDFGQGFYYADL
                     MTEREVVNLLNAIARSTRRDNKEQEKEERRAERKAEKEREREEKEREKTGALSNSEAR
                     SEVADSGSPDKKAGRTIRRRRGSSEESKSTNGNGAANGASPREIQQPPSPGEGFRTAS
                     QPETSRP"
BASE COUNT          453 a          629 c          638 g          346 t
ORIGIN      
        1 ggcgtcacgg tcacgcaata cgcttttcag agcggcgagg taggaggcac gggcgaagag
       61 gcaggccaat tcaagctgcg cgtcatggct ctggaggcat ctggcgcaag cgtttgggaa
      121 tgggatgccc gccgcaacga aatcaacacc ggttccgaag tggaacacgc acttggtttc
      181 tcgcccggga cacttcgatg cgccgccgac agctggctcc agcatcttca cgcctctgac
      241 agagagcgga tgcggctcac tctgtggacg ttgcgcgaac gccagggcgg cgacatcaac
      301 caggacttcc gcatgaagcg cgccgacggc ggctatctct ggtttgagct acgggcggcg
      361 gtgacgcaac agcgggcgac gagaacgctg cgcggcgtgg gcttgttgcg aaacgtgaac
      421 gcccagaagc gcgcgcaaga gcgtctgttc cataacgcca ttcgggatgg cctgacaggc
      481 ttgccaaaca gggagctttt cctcgacagg ctggaaacag cggttgcgcg cgcgaagaac
      541 gaacagacca agcccacggt gttcttcatc gacctggacg ccctgaagaa ccagcgctcg
      601 ccggacttta ccacaaatga cggcgtgctc ctcaccatcg cccggcgtct tacccgccac
      661 atctccccgc aggatacgct cgcgcgggtc agcgcgctgc aatttgcgat cctcatctcc
      721 gaagacaccg aaccgcgcca catcgcaatg ctcgcggaac gtgtccgccg gtccttgcgc
      781 acgcccatga aggttggcgg ccgcgatctg gttctgaccg gcagcatcgg catcgcgatg
      841 tatgacggca cccaggaaca cggcgaagac ctgcttcgcg aaggcgaaag cgccatgtat
      901 cgcgccaagc gcgccggttc ggaccggatc gagctgttca aaccagaaat gcggggcgcg
      961 gccgacgagc gctccatcgt gcagtccgat ttgaagcacg caatcgagcg ccgccagatt
     1021 cgcgtcctct accagccggt gatgcggatt gcggatgagc gactcgccgg catggaggcc
     1081 ttcgttcttt gggagcatcc gacgcttggc cgcctgagcc tgccggagtt cgaggccgcg
     1141 gccgaatcag ccgggctctc gggcgaatta tgggcctatg tcttcgagcg gtgcatccgg
     1201 cagggcgccc gctggcatcg cattctccag cgggaagacc cgatctatat cagcatcaac
     1261 atgtcgagcc agcaactctt tcacaacgac cttgtgcaga acttgcggct catcatcggt
     1321 cgcgaaacgc tgccgaaggg cgcattgagg ctggaaatcg ccgagacgct gatcaactcg
     1381 aatccggaac aggccatcga gatcctggac tggcttaaga gcttgaatgt gagcgttgcg
     1441 ctcgacgagt tcggcgtcag cttctcatcc ttgtcctatt ggcaccgcct ttcgatcgac
     1501 gccatcaaga tcgaccgctc gcttgtcgca atgagcgaca aagaacggtc cagcgcgatg
     1561 gtgctgaagg cggttttgac gatcgcccat gagcttgcaa aagacgtcat cgccgtgggc
     1621 gtcgacaacg aagaagacct cgcttatgtc cgcgcgcttg gctgcgactt cggccagggc
     1681 ttctactatg ccgaccttat gacggagcgc gaggtcgtca atcttctgaa cgcgatcgcc
     1741 cgttccacgc gccgggacaa taaggaacag gaaaaagagg aaagacgagc cgagcgcaaa
     1801 gccgaaaagg aaagggagcg ggaggaaaag gagcgggaaa aaactggagc tctttccaac
     1861 tccgaggcga ggtccgaagt ggctgattct ggttccccgg acaaaaaagc aggcaggacc
     1921 atcaggcgcc gccgaggctc cagcgaagaa tcgaagagca cgaacgggaa tggagcggca
     1981 aacggggcat ccccgcgcga aattcaacag cccccgtcac ctggcgaagg ttttcgcacg
     2041 gcctcacagc ccgaaacctc aaggcc
//
LOCUS       DATMGX010000656         2009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_261122, whole genome shotgun sequence.
ACCESSION   DATMGX010000656 DATMGX010000000
VERSION     DATMGX010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261122"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VNR65_10700"
     CDS             complement(<1..181)
                     /locus_tag="VNR65_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19184.1"
                     /translation="MNLLDISPDEFVRLSERVVGIAADYLRGIDSRSIVPAEGGAEIE
                     KLYRTELPEQGLAGKA"
     gene            647..1186
                     /locus_tag="VNR65_10705"
     CDS             647..1186
                     /locus_tag="VNR65_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWJ19185.1"
                     /translation="MHTLSQEPLAATERVFIDQVVARLADRPGSLLGILEAVQEHNPH
                     KYLPPETLEYVAARTETPLSRIYSVATFYALFNLQPQGDNTICICRGTACHTRGSRNL
                     LQSVRLALGIGSLDSSEESEADKILQTTPDNKFTVRTVACFGQCALAPVVEVNHRICG
                     HVNERTLQREIRTIERESE"
     gene            1186..>2009
                     /locus_tag="VNR65_10710"
     CDS             1186..>2009
                     /locus_tag="VNR65_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19186.1"
                     /translation="MPRILDIGAFNAVRESGLAKLVPSVPRIAVGMGTCGRGNGAEGL
                     YHAFAEAIERSGINAVLANVGCFGACFQEPLANVRLPGAPLVIYRRVQAADVTRILHD
                     ISLGSLTPDLVYCKVEEWDHITAHVKYGRGYPEVPAWNDVPFFKGQKKIVLRNCGFIN
                     PDDIEEYIAVGGYQALYKVLIDGRPENVIEQIKAAKLRGRGGAGYLTGNKWEFLAKAK
                     ADQKYIICNADEGDPGAYMNRNEIESDPHSLLEGMIIGAYVMGATEGIIYVRAEYPL"
BASE COUNT          433 a          614 c          520 g          442 t
ORIGIN      
        1 cggcctttcc cgccaaacct tgttctggca attctgtcct ataaagtttt tcaatttctg
       61 ctccaccttc ggcaggaaca atcgagcgtg aatcgattcc gcgtaaatag tcagcggcaa
      121 ttcctacgac acgttccgat agtcgaacga attcatccgg tgaaatgtcc agtagattca
      181 tcgaactcga tcttacacta cagtccaacg cgtagatgag ccctgtattt cgaccttcga
      241 atgaaaatgc aatcctgatg ttttgcaacg ttcccatcca gatcagctgt gaaataagag
      301 aagtgaccca tctggatcgc gtcttgtgcc taagtgtcac ctcgactgat ggcggcaagc
      361 gctttggatt gatagaggta gacctgccac gaatgcaaga gcccagccct cgtcctaact
      421 cacccctctg ggtgactccc atcactgcgc tatcgcccgc ccgggcctta actctctact
      481 aggcacgtcc tttgcctgcg gtaggcgttt gtgagcgaac acctttcgcc cgcctccgat
      541 tcccgtgtgc cgcattcgct tcgcgctcgc tcctgcgatt ttgtcgcgcg atgcgaatgc
      601 attgttggat ttgcagagaa gtgctggtcc cttgtgaagg agcaccatgc acacactgtc
      661 ccaggaaccg cttgccgcca cggagcgggt ttttatcgat caagtcgttg ccaggctcgc
      721 cgatcgtcct ggatcacttc tcggaatcct tgaagccgtc caagagcaca acccgcacaa
      781 atatctcccg ccggaaacct tggagtatgt tgccgcaaga acggagacgc cgctctcgcg
      841 catctacagc gtcgccacgt tttatgcgct cttcaatctg cagccgcaag gtgacaatac
      901 gatttgcatt tgccgaggca ccgcctgtca cacccgcggc tcgcgcaacc tgctgcaaag
      961 cgtgcgtctt gcactcggta tcgggtctct cgattccagc gaagagagtg aagccgacaa
     1021 gattctgcaa accactcccg acaacaaatt taccgtgcgc actgtcgcct gtttcgggca
     1081 atgcgcgttg gctccggtgg tggaagtgaa tcaccgcatt tgtggccacg tgaacgagcg
     1141 caccctgcag cgcgaaatcc gcaccatcga acgggagagt gagtgatgcc gcgcatcctc
     1201 gacattggcg cattcaatgc cgtccgcgaa tcgggactcg ccaagctggt cccctccgtc
     1261 ccgcgcattg ccgtcggcat gggcacctgc ggccgcggca acggtgccga gggcctctac
     1321 cacgccttcg ccgaggccat tgagcgcagc ggaatcaacg ccgttctcgc caacgtcggc
     1381 tgcttcggcg cctgttttca ggaaccactc gctaatgtcc gcttgcccgg cgccccgctc
     1441 gtaatttacc gccgcgtgca agctgccgat gtaacgcgta ttcttcacga catttccctc
     1501 ggcagtctca cgcccgatct cgtttactgc aaagtcgaag agtgggacca catcactgcc
     1561 cacgtcaagt acggccgcgg ctatcccgaa gttccggcct ggaatgatgt tccattcttc
     1621 aaaggtcaga agaaaattgt tcttcggaac tgcggcttca ttaatccgga tgacatcgag
     1681 gaatacatcg ccgttggcgg ctaccaggca ctctacaagg tcttgatcga tggacgcccc
     1741 gaaaatgtca tcgagcaaat caaggccgcc aagctgcggg gacgtggcgg cgcaggctac
     1801 ctgaccggca acaaatggga gtttcttgct aaagccaagg ccgatcagaa atacatcatc
     1861 tgcaatgccg acgaaggcga tcccggcgcg tacatgaatc gcaatgagat cgaaagcgat
     1921 ccacactcgc tgctggaagg catgatcatc ggcgcctacg tgatgggtgc aacggaaggc
     1981 atcatttatg tgcgcgcgga gtatccgct
//
LOCUS       DATMGX010000657         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_36427, whole genome shotgun sequence.
ACCESSION   DATMGX010000657 DATMGX010000000
VERSION     DATMGX010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36427"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VNR65_10715"
     CDS             <1..301
                     /locus_tag="VNR65_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19187.1"
                     /translation="NVSIDSRHLMKNNLQRKPQPGRTAYRTLSGWALGTLIEHGAVAE
                     CEHHGHRRDRRDPDAWNRARDAAREAPFLGTSEEQSLAALDEVMRSIGENCPDCD"
     gene            370..1224
                     /locus_tag="VNR65_10720"
     CDS             370..1224
                     /locus_tag="VNR65_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWJ19188.1"
                     /translation="MAPRANWKGFLRLSLVTCPIALFPATSESEKISFNQINKKTGNR
                     IRYLKVDADTGDEVENADIIKGYQVDKDRYLEVTKDELENIALESTRTIEIDEFVPRS
                     EIDDLYLVRPYYIVPDGKVGHDAYAVIRETIRSLDKVAIARVVLTSREHIIALEARDK
                     GLMGMLLRYPYEVRDAAEYFDDIQDVKITKDMLDLAKHIVEQKSGHFEPDKFEDHYEA
                     AMQELLTRKQKGLPITAAKREAPGNVVDLMSALKASIAGGKRTPAAGKPPAKAAKKSK
                     PAARRKAS"
     gene            2105..>2351
                     /locus_tag="VNR65_10725"
     CDS             2105..>2351
                     /locus_tag="VNR65_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19189.1"
                     /translation="MPLDAIDEEALRTGRPVGRFVGDTYRYSMPIIQGSTVAARKEVC
                     AACHTALMSENDGEVIAVFSSSLSTTEGMAALHRSLKL"
BASE COUNT          528 a          672 c          694 g          457 t
ORIGIN      
        1 caacgtgtcc attgattcga gacacctcat gaaaaacaac cttcaacgca agccgcagcc
       61 gggccgcacc gcctatcgca ccctgagtgg ctgggccctc ggaactctga tcgaacatgg
      121 cgccgtggcg gaatgcgagc accatggcca tcgccgcgac cgcagagatc ccgacgcctg
      181 gaaccgggcc cgcgatgcgg cacgggaggc gccatttttg ggaacctcgg aggagcaatc
      241 gctggcggct ctggatgagg tcatgcgtag catcggcgaa aactgcccgg attgcgactg
      301 atcggactcc acttccggcc gcgcgcgcct atatcctgag tctgtttcag actagggagt
      361 attgcgtaca tggccccgcg cgccaactgg aagggatttt tgcggctatc cctcgtgacc
      421 tgtccgatcg cgctgtttcc ggcgacgtcg gaatccgaaa agatcagctt caaccagatc
      481 aacaagaaaa ccggcaaccg gatccgctat ctgaaggtcg acgcggacac cggcgacgag
      541 gttgagaacg ccgacatcat caagggctac caggtcgaca aggaccgcta tctggaggtc
      601 accaaggacg agctggaaaa catcgcgctc gaatccaccc gcaccatcga gatagatgag
      661 ttcgtgccgc gcagcgagat cgacgatctc tatctggttc ggccttatta catcgtgccg
      721 gatggcaagg tcggccacga cgcctatgcc gtgatccgag agaccatccg ctcgctcgac
      781 aaggtggcaa tcgcgcgcgt ggtgctgacc agccgcgaac acatcatagc gctggaagct
      841 cgtgacaagg gcctgatggg aatgctgctg cgctacccct atgaggtgcg cgacgcggca
      901 gaatatttcg acgatatcca ggacgtcaag atcacaaagg acatgctgga tctggcgaag
      961 catatcgtcg aacagaaatc cggccatttc gagccggaca agttcgagga tcactatgag
     1021 gccgcgatgc aggagttgct gaccaggaag cagaaggggc tgccgatcac agctgccaag
     1081 agggaggcgc ccggtaatgt cgtcgacctg atgtcagcgc tgaaggccag catcgcgggc
     1141 ggcaagcgca cgcccgcggc tggcaagcct cccgccaagg cagcgaagaa atcgaaaccg
     1201 gccgccaggc ggaaggctag ctaacatcgc cgtggcaatt tcctcgcgct acctcccctg
     1261 cattccgagc agcgggactc ttgccgatta tctcaccacg cgcatttggt aaaaattggg
     1321 agccgagtcc gacgcccctg ggccatagat cgtccaactg ggtaagaact tgcttactgc
     1381 tgcttcatcg ccacgctgcg ggattgggct cgtttgggat tgatgttggc aaacgatggt
     1441 gtctggaatg ggcaccagat cattccgcgc cagtgactac gggatgctac ctcacttgat
     1501 tcgagggcgg tgccgtcggg tagttactta cgcaaatcag accggccctt tggtccgttt
     1561 cgtgctgcta ggaacagact catgcaccgc agcaaacagc ggagcgaaca ccttttcggt
     1621 gcgctcctaa ggccgctagg acaaggtact tgggcagcta cttgactttg atcaatggcg
     1681 gatcgttttc ccccatttaa tatctaatca atgcgggtct tgccccttga ttcaaagagc
     1741 ccgcgcgggc ggaaacattg ttctcttggt gggaaaatat agggctccag gtccgcttca
     1801 tggtcattgc cagcgttggc cttttgacac ttgcgatcgg tgccttagct gttgtcgatt
     1861 ggtacgaatt ttcaagtttt gaagaaaggt taaaagcgtt ctccgagaat gaactccgat
     1921 cattaaactc tctggtcgag agcgcgatgg aacgacgacc gggagaccag aaaaacgttg
     1981 ccatcaaggt cttcaatggc tggttcgaga gccgcaacaa ggagtatttc gggaagctct
     2041 ggagcgtttg ggccccaaga cggcggccta cattgcgaga acggctccgg agctcgcgcc
     2101 aaaaatgcca ctggacgcta ttgacgagga ggcgctgcgc accgggcgtc ccgtcggacg
     2161 cttcgtcggg gacacctatc gctatagtat gcccatcatt caaggcagca ccgttgctgc
     2221 ccgcaaagaa gtctgcgctg cctgccacac agcgctgatg agcgagaacg acggcgaagt
     2281 gatcgcggtg ttctccagca gcctgtcgac gaccgaggga atggcagctt tgcaccgctc
     2341 gctaaagttg a
//
LOCUS       DATMGX010000658         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_437188, whole genome shotgun sequence.
ACCESSION   DATMGX010000658 DATMGX010000000
VERSION     DATMGX010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437188"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /locus_tag="VNR65_10730"
     CDS             complement(<1..1729)
                     /locus_tag="VNR65_10730"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWJ19190.1"
                     /translation="MKNYEGANIRNVALVGHSHSGKTSLASAMLFTAGATQRLGRVDE
                     GTTVTDYDEEEISRGMSISAATAFVEWGKAKINLVDTPGFNMFIHEAEMVMPAVEAAL
                     VVVDAVSGVEVVTERIWHYYDRVALPRVIVCTRTDRERADFNRVMESLTTAFGRTVVP
                     VQLPIGSEKSFTGVVDLVKMQAYTYEMGGNGKSTVGPIPAALEEQAKAAHERLVELVA
                     EGDDALMEEFFDKGTIGEEHIVAGLHNAIREDKIFPVLFASGLGNIGTDEVLDFIVDY
                     LPTAVERGGVEAAAAANNGEPPTRKISDAEPLSLFVFKTISDPFSGRISFFKVYSGVL
                     KNDATLQNFSRGSTEKFAHLSVMQGKTAVPVNELHAGDIGAVAKLKDTLTGDTLGDKG
                     SPIEYPKVTFAEPAITFAIEPKTRADEDKLSNGIHKLMEEDAMLRFFRDAQTKEFLIA
                     GTGQQHIEVIVAKLKNRYHTEVNLKAPKVPYRETIRGKADVQGRHKKQSGGHGQFGDC
                     KIKMEPMPRGGNFEFVNEIFGGSIPKNYIPAVEKGIVEAAQRGFLAGFPVVDFRVILY
                     DGSYHDVDSN"
BASE COUNT          399 a          611 c          633 g          444 t
ORIGIN      
        1 cgttggagtc cacgtcgtgg taggagccgt cataaaggat gaccctgaag tccaccaccg
       61 ggaagccagc gagaaagccg cgttgtgcgg cttcgacaat gcccttctcg acggcgggaa
      121 tatagttctt gggaatcgag ccgccgaaga tctcattgac gaactcgaag ttgccgccgc
      181 gcggcatggg ttccatcttg atcttgcaat cgccaaattg gccgtgtccg ccgctctgct
      241 tcttgtggcg tccctgcacg tcggccttgc cgcgaatggt ctcgcggtag ggaactttgg
      301 gggccttgag gttcacttcg gtgtgataac ggttcttgag ctttgcaacg atgacttcga
      361 tgtgctgctg gccggtgccc gcgatgagga actccttggt ctgggcatcg cggaagaagc
      421 gcagcatggc atcctcttcc atcagcttgt ggatgccgtt gctcagcttg tcttcgtcgg
      481 cgcgggtctt gggctcgatg gcgaacgtga tcgccggctc ggcgaacgtg accttcggat
      541 actcgatcgg agatcccttg tcgcccaagg tatcgccggt cagggtgtcc ttcaacttcg
      601 caactgcgcc aatgtcgccg gcgtggagtt cgttgaccgg gaccgctgtc ttgccttgca
      661 tcaccgaaag gtgggcgaac ttctcggtgc tgccgcggct gaagttttgc agggtcgcgt
      721 cgttcttcag cacgccggaa taaaccttga agaaggagat gcgtcccgag aacggatcgc
      781 tgatggtctt gaatacgaac agggagagcg gttccgcgtc gctgatcttg cgggttgggg
      841 gctcgccatt gttcgcggcg gcagcggctt ctactccgcc acgctcgacc gccgtcggca
      901 agtaatcgac gatgaagtcg agcacctcat cggtgccaat gttgcccaga cccgaggcga
      961 agagaacggg gaagatcttg tcctcgcgaa tggcattgtg cagtccggcg acgatgtgct
     1021 cctcgccgat cgtgcccttg tcgaagaact cttccatcaa ggcgtcgtcg ccttcggcaa
     1081 ccagttccac cagccgctcg tgcgcagcct tggcctgctc ctccagggcg gctggaatcg
     1141 gcccgaccgt tgacttgccg tttccgccca tctcgtaggt gtaagcctgc atctttacca
     1201 ggtccacgac gccggtgaaa cttttctcgg agccaatcgg caactgcact ggcacaaccg
     1261 tccgtccgaa ggccgtagtg agcgattcca tgacgcggtt gaagtcggcg cgctcgcggt
     1321 ccgtccgagt gcacacaatg acccgcggca acgcgacccg gtcgtagtaa tgccaaatgc
     1381 gttcggtcac cacttccacg ccactgaccg cgtccaccac gaccagggcg gcttccaccg
     1441 caggcattac catctcggct tcatggatga acatgttgaa accgggcgtg tcaaccaggt
     1501 tgatcttggc cttaccccac tcgacgaaag cggtggcggc ggagatggac atcccgcgcg
     1561 agatctcttc ttcgtcgtaa tcggtgacgg tggtgccttc atcgacccgg ccgaggcgct
     1621 gggtggcgcc ggcagtgaaa agcatcgccg atgcgaggga agttttgccc gaatgtgaat
     1681 gcccgaccag ggcgacgttg cggatgtttg cgccttcgta attcttcacc tagtgactcc
     1741 ttcgagtggg ccgtggcaac accgtgcgac ggcggcatct ccacctggtg ctcagaggca
     1801 ttgttcccca gtagcacgcg attctcgtgc ttgcacaacc ccgaggttgc cggtgaggca
     1861 aaaaggcggg gcaccatgcc agcctgcaca tggtgaaact gaacaaactt agaatcctag
     1921 cacaacgagt ttaccgcttc caccgcaagt cttttccaat acaagatgag cctgaaggag
     1981 gaggcagttt ccaatacaag atgagcctgg agcgggtggc atgctgagga ttgacaacca
     2041 gcgtgcacga agcagagaag ctgaacctgg agcagatcga ggctttt
//
LOCUS       DATMGX010000659         3641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_179181, whole genome shotgun sequence.
ACCESSION   DATMGX010000659 DATMGX010000000
VERSION     DATMGX010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179181"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VNR65_10735"
     CDS             complement(<1..1073)
                     /locus_tag="VNR65_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWJ19191.1"
                     /translation="MSFERILRWALVSIAVTGLAAGIAAHAVGRQDLADLCWWFATAP
                     VVGGLAVSIVRDFMAGRLGVDAIALVSMSAALALGQPLAGAVVALMYSGGNVLEDIAV
                     ARAEHDLRSLVDRAPREAHRLIDDHIEEVPISDVAVGDRLLVRAGEIIPVDGVLGSSA
                     ATIDESALTGEPIPVVKTRGAAILSGSLNAGETFELAVSAAAGESTYAGIVRLVTAAQ
                     TAKAPFVRLADRYALIFLPVTLVLAFVAWLISGDLIRSLAVLVAATPCPLILAAPVAF
                     IAGVAQAARRGILVKGGGPLEALARAHTVLFDKTGTLTVGGARLLSVEVAPGESADEV
                     LMLGASLEQASHHVLAAAIVQAG"
     gene            complement(1693..2052)
                     /locus_tag="VNR65_10740"
     CDS             complement(1693..2052)
                     /locus_tag="VNR65_10740"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HWJ19192.1"
                     /translation="MKTITALIALAVGIALATPVLAEDDQTSGNYWLHECKKDPKQLA
                     QSSGSFQYGWCFGVVYGLSIHPLICVPTQVPVVQEMDVVLKYLDVHPETRHKSFRLLA
                     LEALKEAFPCADGEKSK"
     gene            complement(2432..3043)
                     /locus_tag="VNR65_10745"
     CDS             complement(2432..3043)
                     /locus_tag="VNR65_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWJ19193.1"
                     /translation="MRKLLCKSGIGIGLALVSSAALAATQEELLGALLGQIQMYVLIA
                     FVIVVIAGVYFMRSRDKRQTPLNEIFKGSRTIHSVGPNASITECVRLMTASKIGALMV
                     MDDGRLIGIFTERDALNKVLASNLDPRNTKVSEVMTKDPYCISPATTVSDAMQLITER
                     RFRHLPIVDNGKVLAVVSSGDLTRWLVQDQLGEVQELVNFAAR"
BASE COUNT          828 a         1119 c         1001 g          693 t
ORIGIN      
        1 atcccggcct gaacgatggc ggcggcgaga acatgatgcg acgcctgctc cagcgaagcg
       61 ccgagcatca gcacctcgtc ggccgattcg ccaggcgcga cttcgacgga aagcagccgc
      121 gcgccgccga ccgtcagcgt cccggtcttg tcgaacagca cggtgtgggc gcgcgccagc
      181 gcctccagcg gcccgccacc cttgaccagg atgccgcgcc gcgccgcctg cgcgaccccg
      241 gcgatgaagg cgacgggtgc agccagaatc agcggacacg gcgtcgccgc gaccagcacg
      301 gcgagcgagc ggatcagatc gcccgagatc agccaggcga caaaggcgag caccagcgtc
      361 accggcagga agatcagcgc ataacggtcg gcgagccgca cgaacggcgc cttcgcggtc
      421 tgcgccgcgg tcaccagtcg cacgatgccc gcataggtgc tttcgcccgc cgccgccgac
      481 accgccagtt caaaggtctc gccggcgttc aaggagcccg aaagaatggc ggcgccgcgc
      541 gtcttgacca ccgggatcgg ttcgccggtc agcgccgact cgtcgatcgt tgccgcgctc
      601 gagccgagga caccgtcgac cggaatgatt tcgcccgccc gcaccagaag ccggtcgccg
      661 acggcgacat ctgatatcgg cacctcctcg atgtgatcat cgatgagacg atgcgcctcg
      721 cgcggcgcgc ggtcgaccag tgagcgcaga tcatgctcgg cgcgtgcaac agcaatatcc
      781 tcaagcacgt tgccgcccga atacatcagc gcgacgaccg cgccggcgag cggctggccg
      841 agcgcgagtg ctgcgctcat cgacaccagc gcaatcgcat cgacgcccaa ccgaccggcc
      901 atgaaatccc ggacgatcga gaccgccagt ccccccacca caggcgcggt cgcgaaccac
      961 cagcagagat cggcgaggtc ctgccggccg acggcgtggg cggcaatacc ggccgccaac
     1021 ccggtaaccg cgatcgagac gagtgcccac cgcaggatgc gttcaaatga cattgcttga
     1081 tccgtgctga tgcagcttac gctgcttttc gcggaaaatg cgctcctcgc gcttgaggga
     1141 aatcaatcgc gcggagccga attgcagcga cttgcaagcg gggaatgata cgcaaatgcg
     1201 gcagaagcac cactgacgcc caccatctgc ggttcgccca gccccgggcg atggggcgca
     1261 aggtcagcga tgagttcacc gtcccgcttt tgtcgaactc agcacgggac aaccatcgat
     1321 tcggagatcg attcggagat gaagtcgctt ggggggagaa aggctattga tcctatcgcg
     1381 acgtcctgga tgctctgggt ctcgacccgg tatatcgaat gaaggccgtt aaaaaatcgt
     1441 ctcacgcacg acgaagctgt ggatggggac gttcgtatct cctgtagcgt cgagtcccag
     1501 atttagaagg actgccacac cagcaactca agagcagtaa ctcgaccacg cgtcggttcc
     1561 ctggtttgtg caattggacc gatcccataa ggaattgcac acgcaaggtg ccgaaatttt
     1621 tgtgcttggc taaccgcatt cagcgggttc agaattgctt ccctcagcgt ctcgctaagc
     1681 gatagctcat gatcatttgc ttttctcacc atccgcacag gggaaagcct ctttgagagc
     1741 ttccagcgcc agaagcctaa atgacttgtg acgcgtttca ggatgaacat cgaggtactt
     1801 caatacgacg tccatctcct gcacaacggg aacttgagtg ggaacgcaga ttagcggatg
     1861 tatgctgaga ccataaacga ccccaaagca ccacccatat tggaaagacc cactcgattg
     1921 tgccagctgc ttagggtctt tcttgcactc gtgtagccaa tagtttccgc tggtctggtc
     1981 atcctccgcc aagacaggcg tcgccagcgc aattccgaca gccagcgcga ttagcgcggt
     2041 gatcgtcttc ataccaaatc tcccttggct caaacgccac gaagcgtcgt cgtttgcgta
     2101 aatcctcgca cattagatcg gttcaaggaa acgaaagcgg acgcttctgg gtatggaaaa
     2161 acaacccaaa agcgtccgca ttttgcatcg aaaatggctc ggaagctcct cagctacaac
     2221 ctctctagtg aaatcatccg cgcagcagcg tctttgactt gaatcaaacg gcgctattcg
     2281 gatggcgcgg ttcgatcata tccctgtttt ggctgaacgc ctcgcagtca gatctaagtc
     2341 gggagcacga ccggcgctac gccgtaagcg gacgttcaac cgacattttt gtatcacgca
     2401 aacatgccat tagcgcaccg cacgattgtc cttaccgggc ggcaaagttg acgagctctt
     2461 gaacctctcc taactggtcc tgtaccagcc agcgggtcag atcgccgctc gatacaacgg
     2521 caagcacctt gccgttgtcc acaatcggca agtgccggaa tcgccgctcg gtgatcaatt
     2581 gcatcgcgtc gctgactgtc gtcgccggac taatgcaata tgggtctttc gtcatcacct
     2641 cggacacttt cgtgttgcgt ggatcaaggt tgctagccaa caccttgttc agcgcgtccc
     2701 gctctgtgaa aatgcctatc agccgaccat catccatgac catcagagcg ccgattttgc
     2761 tagcggtcat taaacgcaca cactcggtga ttgaggcgtt aggcccaact gagtgaatcg
     2821 tcctgctccc cttgaatatt tcattcaatg gcgtctgacg cttgtctcgt gacctcatga
     2881 agtaaacgcc ggcgataacg acgatcacga atgcaatgag cacatacatt tgaatttggc
     2941 ccaaaagcgc ccccagcagc tcttcctgag tggcagcaag tgccgcagag gaaaccagcg
     3001 caagaccgat tccaatgccc gatttgcaaa gaagctttct catcaccgtt gatcctcgcc
     3061 attctccgcc tcggcgcccg ccccgatgaa gcagcggacc caatggtaaa aaaagccgtg
     3121 aacttaacca gaccgcgcag cgatcaagct gctacagtga tcgggactgg aagtccgcca
     3181 agagtagcgt tgctacaaag gccaatacgg cgactaggac gactgaaaac aaaatcgaat
     3241 cgatgggcat gattatctcc tcctcggacg tttttttgtc gttaatcaag gcccagccac
     3301 acctgatgac tcatgagcaa cttggttgca ccgctggtct cggcattctc tgaagagaga
     3361 agcggcgccg cccgcggaac tgaaccggcc aacaaccttt cgccgagccg aaagttgatt
     3421 cgaaaattag gctttcagca tgaaagggcg aggacgcgct tcgcttttgc atgacgctct
     3481 ccttgtttgt tttcaactaa aacagtcgtg cgcgggcata acgagattgg tagactatgg
     3541 ttgcaatgca acaatcagca cgcagaacca gcactgctgc aagacccttt tgactactgc
     3601 ggcaagcgcc gcatcgacag aagtcggtgt acggcggcga t
//
LOCUS       DATMGX010000660         2382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_330779, whole genome shotgun sequence.
ACCESSION   DATMGX010000660 DATMGX010000000
VERSION     DATMGX010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330779"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VNR65_10750"
     CDS             <1..518
                     /locus_tag="VNR65_10750"
                     /inference="COORDINATES: protein motif:HMM:NF023711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3623 family protein"
                     /protein_id="HWJ19194.1"
                     /translation="FAALLGVVICFSTMNGQNHVGSWTYTIIWLMHTSAKLNAVLGAR
                     NLNEHFFPEQLRYLSSFIKKRPMNLLFPVSITVSTVAVAVLVNKAMMAESNSFELLAY
                     TFYIVILALGILEHWFMMLPLPSEALWNWAVKAKKALSGPEHEGVLSDALDATPQPAR
                     ARVPAVAGQDI"
     gene            complement(827..1156)
                     /locus_tag="VNR65_10755"
     CDS             complement(827..1156)
                     /locus_tag="VNR65_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19195.1"
                     /translation="MTSSLFFCGACAGGSPELKADEKLAQIPLFPEFCDEGAGGAGAL
                     DGMSEELLPPMLVRDVMPPDVAEQPAKAVQNNNKRTARNIVTPTYMHTMPNYCYNSLN
                     SALQEAD"
     gene            1155..1550
                     /locus_tag="VNR65_10760"
     CDS             1155..1550
                     /locus_tag="VNR65_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWJ19196.1"
                     /translation="MTGLDPDEALRLVNEYRSSKGLPALSLESHATAAAAILAKDMAA
                     HDRMSHAGPNGADLQKRLTTAGYRYKVAAENVGVGQKSFAEIVEGWKKSMPHSRNMLL
                     AEAKHMGVAYEYKPDTQYKTYWTLVVAAP"
     gene            1640..>2382
                     /locus_tag="VNR65_10765"
     CDS             1640..>2382
                     /locus_tag="VNR65_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWJ19197.1"
                     /translation="MSSPSSTAYLPQAVAAHPHGHARALALPALGVVFGDIGTSPLYT
                     LKVVLGAAGSHAADRAIVLGSLSLILWTLIIVTTIKYVGVAMRVDNHGEGGIMALMAL
                     LTGRKGLGSGRTKKKAFIVGVGLIGAALIYGDGVITPAISVLSALEGLSVITSRLEHF
                     VVPATVLILILLFLFQPQGTARIGKLFGPVMALWFLVLGGLGLWGIVQNPMVIAAANP
                     IYGLNYLASGGMTSFLVLGAVFLCVTGAEA"
BASE COUNT          484 a          681 c          680 g          537 t
ORIGIN      
        1 tctttgcggc cttgcttggg gttgtgatct gcttctcgac gatgaatggc caaaatcatg
       61 tcggctcctg gacctacaca attatctggc tcatgcacac gagcgcgaag ttaaatgcgg
      121 tgctgggcgc aagaaatctg aatgaacact ttttcccgga gcaattgcgc tacctgtcga
      181 gctttatcaa aaagaggccg atgaaccttc tgttcccggt gtcgattacc gtttccacag
      241 tcgcggtcgc ggtcctcgtg aacaaggcga tgatggcgga gagtaactca ttcgagttgc
      301 ttgcctatac cttctatatt gtgatcctgg ctttggggat tttggaacac tggttcatga
      361 tgctccccct tccgtcagaa gccctttgga attgggccgt caaagccaag aaggcgcttt
      421 cgggacctga acatgaggga gtgctgtcgg atgctcttga cgcaacccct caaccagcac
      481 gcgcccgtgt tcctgccgtg gccggacagg atatataata gagccgtcat gcgagttcgc
      541 ataaccgctt ggcactcatt cgatatctga aaaacaaata ggttagctcg gcctctagcg
      601 aggccgacgc caatttaaga ccccgccatg atagacgctc agcgcctgcc aggaacgcca
      661 gccgttcggc gcatacaaaa tgccggttga ctgcaagctg ttctcccgcc agcggcgctt
      721 gggcggacgc aggtccatcg tcacaggccg ggagggtgcg ccgcggtcgg aacgttaagc
      781 gattccctat ttagtgaaat tatcatcatc ttgacgctat ccgggtctaa tccgcctcct
      841 gcaaggctga attaagcgag ttgtaacagt aattcggcat tgtatgcata tatgtggggg
      901 tcacgatgtt gcgtgctgtc cgtttgttgt tgttttgcac ggccttggcc ggttgctcgg
      961 cgacgtcggg cggcattacg tctctgacga gcataggcgg aagcagctct tccgacatgc
     1021 cgtccaaggc gcccgcgccg ccagccccct cgtcgcaaaa ctccgggaat agcggtatct
     1081 gggctaattt ttcgtcggct ttcagctctg gcgagccgcc ggcacaggct ccgcaaaaaa
     1141 atagcgatga ggtcatgacg ggtctggacc cggacgaggc acttcgcctt gtcaacgaat
     1201 accggtcgtc aaagggcctg cctgccctct ccttggaaag ccatgccacg gcagcggccg
     1261 caattctcgc caaggacatg gccgcgcacg accgcatgtc tcatgccggt cccaatggag
     1321 ccgatcttca aaagcgcctg accacggcgg gatatcgcta caaggtggcg gccgagaatg
     1381 tcggcgtcgg ccagaagtcc ttcgccgaga tcgtggaggg atggaaaaaa agcatgcctc
     1441 acagccggaa tatgctgctc gccgaagcga agcatatggg tgttgcctac gagtataaac
     1501 cagatacgca atataagacg tactggacac tcgttgtggc tgcgccttaa tcagagtcaa
     1561 agaaactgac ttaatgcggc tgctataatg ccatggtaaa agctgcacat ctcctcgcag
     1621 caccataccc ggatcaagaa tgagtagtcc aagctccacg gcttatctgc cgcaagccgt
     1681 cgcagcgcat cctcatggcc atgccagagc actggccttg ccagccctcg gcgtcgtttt
     1741 cggcgacatc ggaacaagcc cgctctacac gctgaaagtt gtgctcggcg cggctggctc
     1801 gcacgcagcg gaccgcgcga tcgtccttgg cagcctgtcg ctgattttgt ggacgctcat
     1861 tatcgtaacc acgatcaagt atgttggcgt cgccatgcgc gtcgataacc atggtgaggg
     1921 cgggatcatg gcgttgatgg cgcttctcac cggccgaaag ggtctgggaa gcggccgcac
     1981 gaagaagaag gcctttatcg tcggcgtggg cctgatcggc gccgcgctca tctacgggga
     2041 cggcgtgatc accccggcaa tctccgtgct gtccgccctc gaaggacttt cggtgataac
     2101 gagccggctg gaacactttg ttgtcccggc aacggtcctc attcttattc tcttgtttct
     2161 ctttcaaccg cagggcacgg cgcggatcgg caaattgttt gggccggtga tggccctatg
     2221 gttccttgtt ctcggcggtc tcggcctctg ggggattgtt cagaacccca tggtgatcgc
     2281 agcggcgaac ccgatctacg gccttaacta tcttgcatcc gggggaatga catccttcct
     2341 cgtgctcggc gcggtctttc tctgcgtcac cggtgcggaa gc
//
LOCUS       DATMGX010000661         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_186288, whole genome shotgun sequence.
ACCESSION   DATMGX010000661 DATMGX010000000
VERSION     DATMGX010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186288"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..192)
                     /locus_tag="VNR65_10770"
     CDS             complement(1..192)
                     /locus_tag="VNR65_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HWJ19198.1"
                     /translation="MRAGDERRDCHDIVQQLGAARTALERATAQLMVSSLSQCIRTAK
                     NGADEAEIQKLMDTFIKLL"
     gene            complement(439..1230)
                     /locus_tag="VNR65_10775"
     CDS             complement(439..1230)
                     /locus_tag="VNR65_10775"
                     /inference="COORDINATES: protein motif:HMM:NF018580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OBAP family protein"
                     /protein_id="HWJ19199.1"
                     /translation="MAAFDHLKNRRGFCRSSILSVLALPITMLLGRTALAEQTEAPVP
                     GSSVSPHSGMLDMAAKTVQTRRPIDAMSEYLNGFHFYANDMGRQVEANHFCTHLSDDF
                     HQCVIYDSNLPNAKLIGIEYIVSERVFKSLPSEEKKLWHSHHFEVKSGELIAPGVPEL
                     AEHDFMNSLVTTYGKTWHTWQIDRDHSFPMGIPQLMMGFTSEGQIKQSILQARDKRFN
                     VSTAERKKNRANIPMPTVDAGANFWQSGKAMQLRLEEVPARTRRA"
BASE COUNT          426 a          635 c          580 g          544 t
ORIGIN      
        1 tcagaggagc ttgatgaacg tatccatcag cttttggatt tccgcttcgt cggcgccgtt
       61 tttggccgtg cgaatacact ggctgaggct gctgaccatg agctgagccg tggctcgttc
      121 aagtgcggtg cgcgctgcgc cgagttgctg cacgatgtca tggcaatcgc gccgttcgtc
      181 gccagcgcgc atcgcgcgca acctgagcgc gacatgacgg gcgatggaag gggttggggg
      241 gaaccgtcgt cggcgagtcg ccgatcactt atattcagcg ccgagtgcgc gagatcgttg
      301 ctaagatgcg cgatttggaa cggccgccgt agcaaacgca tctgccgaag ccttgcatgg
      361 cttcccgatg ctcttccagc aaccgcgaag tgtcttccgc cgatcgtagg tcatccagct
      421 tgtcaggatc agtctccatc acgctcgtcg cgtacgtgcc ggaacttctt caagcctgag
      481 ctgcattgct tttccgctct gccaaaaatt ggcccccgca tcgacggtcg gcatcggaat
      541 attcgcgcga ttcttttttc gttctgcagt agatacgtta aaccgcttgt cgcgcgcttg
      601 aaggatcgat tgctttattt ggccctcgct ggtgaagccc atcatcagtt gcgggattcc
      661 catagggaaa ctgtgatcgc ggtcgatctg ccacgtgtgc cacgttttgc catacgtcgt
      721 gacgagactg ttcatgaagt cgtgctctgc caattctgga acacctggcg cgatcagctc
      781 tcccgacttg acttcgaaat ggtggctatg ccagagtttc ttttcttcgg agggcaacga
      841 cttaaagacg cgctcgctaa cgatgtattc gatcccgatc aatttcgcgt ttggaaggtt
      901 tgagtcgtaa atgacgcatt ggtgaaaatc gtcgctcaga tgggtgcaga aatgattggc
      961 ctccacctgt cgccccatgt cattggcata aaagtggaag ccattaagat attcgctcat
     1021 cgcatcaatg ggtcgtctgg tttgcaccgt ctttgcagcc atatcgagca tgccgctgtg
     1081 cggggaaacc gatgacccag gaacaggcgc ttcggtctgt tcagccagcg cggttcgacc
     1141 gagaagcatg gtgatcggca aggcaaggac cgacaggatg ctggatcgac agaacccgcg
     1201 gcgatttttc agatggtcaa atgccgccat aacgcctccg ttctgagacc gtcgttcggg
     1261 aggtgcatgc ggcagccctc cggcctgatt cttagtggtc ttcccaatgg gctatttttg
     1321 ccgccaggtc ggccggaggg atttcacctc cagccgccta cagaaccgtg cgtaaatctc
     1381 tcgacttaca cggctcgtct caactatttc tcccgcaacc tcacgatcac gggcctacgc
     1441 agaaagatga tcgctcctct cgatttctcg attggcgaag actttctttg agttgatccc
     1501 tccccttcgc tccacgctca ttacaagcgc ttcggcgcta catacgggag agtccgcccc
     1561 cgtcatgtgc atcggtactt tcccccttcg tggttcgcac ttataggatt ttcccttcgc
     1621 atcacatgac gagttcccaa gttccgtacc caagcctgaa tgagagtcat gccgcctgaa
     1681 caccggacac cacatggccg gtaggtaggt ttccgccatg ctgctcccgg ggcagggatg
     1741 cgaccccggt ttcgatgtcg tctatctttc tttcgatgcg tcgtcggtcg gtttgctttc
     1801 gctcatctct ctcactcata catgccgtga tcttccacgg cttttaccgt aacgttcacc
     1861 accgccgtct ttcgacagaa gcagcttacg gctgtttgaa gcatgttccc acaaaccggc
     1921 ttcgaagggc catccttcat cttgggtaca gcatcacgct tgctagcgtg ttcatggcac
     1981 agcatcccct cgaaaagttc cgaagcgtta aaactacgac ttcagtaggc gtttatcgct
     2041 tctcgataag ccgccgcgcg gtcacttttg acatttcgcc ggtcggatcg gtcgaaaaac
     2101 gcgccggaat cctccgttcg gcggcgctcc agcgcggaca ctttgaacaa aaagcatgtt
     2161 ttgtcaaaag ctccgggcgg aaatc
//
LOCUS       DATMGX010000662         2200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_291239, whole genome shotgun sequence.
ACCESSION   DATMGX010000662 DATMGX010000000
VERSION     DATMGX010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291239"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..804
                     /locus_tag="VNR65_10780"
     CDS             97..804
                     /locus_tag="VNR65_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ19200.1"
                     /translation="MPDNILDVRGLWVAYGATPILQGVDMTVRRGEIVGVIGRNGAGK
                     TTLMRCLIGLIRATRGSVSLFERDITSLPADARARAGIGYVPQGRDVFPQMTVEENLE
                     VGKLIGGPDARKLPDLVYTYFPQLKERRRQIAGTMSGGEQQQLAIGRALIGNPILMLL
                     DEPSEGVQPSIVQMICEVLKSIRDEIGTTIVFVEQNLDTILAIAERCYVMDKGRISTT
                     LSEDCIDADHVRAQLLI"
     gene            1056..1508
                     /locus_tag="VNR65_10785"
     CDS             1056..1508
                     /locus_tag="VNR65_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19201.1"
                     /translation="MRGRSQLTSNDRWFFIQLYRWFPSILQVLTIIRPDTLVRWHRAG
                     FRRYWRWKSRPRGGRPQIETELRVLIRRMSVENPLWGAPRIHGELLKLGFELAQSSVA
                     KHMVKRRGPPSQGWRTFLHNHAPDIAAMDLFVVPTMASTCSMPSSSSG"
BASE COUNT          454 a          622 c          644 g          480 t
ORIGIN      
        1 ccgtgcttca tctgggcaag gtgttcgcga gaggctcgct cgacgagatc attcgcgacc
       61 cgggggtggc tgaaatctat ctcgggaaga cacatcatgc ctgacaatat cctggatgtt
      121 cgcgggttgt gggtcgccta cggcgcgact ccgatcctgc agggcgtgga catgacggtg
      181 cggcgcggcg agatcgtggg cgtcatcggc cgcaacggcg cgggcaaaac cacgcttatg
      241 agatgtctga tcggactgat ccgtgcgacg cgcggctctg tcagcctgtt cgagcgcgat
      301 attaccagct tgccagcgga cgcgcgggcg cgcgccggca taggatacgt tccgcaaggc
      361 cgcgatgtct ttccgcaaat gaccgtcgag gaaaatcttg aggtcggcaa gctgatcggc
      421 ggtcccgatg cgcgcaagct gccggatctc gtctacactt attttccgca gctgaaggag
      481 cggcggcgtc agattgccgg caccatgtca ggcggggaac agcagcagct tgcgatcggg
      541 cgcgccctga tcggaaaccc gatcttgatg ctgctggatg aaccgtccga aggcgtccag
      601 ccgtcgattg tccagatgat ctgcgaggtg ctgaaatcca tccgagacga aatcggaacg
      661 actatcgttt tcgtggagca aaatctcgac actattctag cgatcgcaga acggtgctac
      721 gtcatggaca agggtcgcat cagcacgaca ctttccgagg actgcatcga tgcggatcat
      781 gttcgcgcgc agcttctgat ctagacgatc gattccaaga aagctgtcat atgggtggga
      841 ctcatctgtc gtttcgaact cctatttagc ggtggagggt gtgtgtaccg aaaaccttat
      901 ccacgttgat gaggtgacag aatcgcctaa gctagtggaa tgatcgggct gctacgtttc
      961 ggtttggcca ttttaatttc gctattcagg tcgaagagcc ggcttgaagc tgagaatgcg
     1021 gtacttcgac accagttgat cgtttgcgac gcaagatgcg cggccggtct cagctcacga
     1081 gcaacgaccg ctggttcttt atccagctgt atcgctggtt tccgtccatc ctgcaggttc
     1141 tcacaatcat ccggcccgac accctcgtgc gttggcatcg ggccggcttt cgccgttatt
     1201 ggcgatggaa gtcacgccct cggggagggc ggccacagat cgagaccgaa ctgcgcgtgc
     1261 tgatccggcg gatgagcgtc gagaacccgc tttggggagc gccacgcatc cacggggaac
     1321 tgctcaagct tgggtttgaa ttggcgcagt cgagtgtcgc caaacacatg gtcaagcggc
     1381 gtggcccgcc aagccaggga tggcgtacct tcctacacaa tcatgcccca gatatcgccg
     1441 ccatggactt gttcgttgtt ccgaccatgg cttcaacctg ctctatgcct tcgtcatcat
     1501 ccggctagac cgaagagacc ttgtctggat caacgtcaca acaagtccga ccgcggagtg
     1561 gattgcgcat cagataacgg aggcctttcc ttgggatgag gctccgcgat acctcattcg
     1621 cgatcgggat cggatctacg gcaccatcgt cacacgccga ttgcgcgcca tgggcatccg
     1681 ggacaagccg acggcatcag cttcaccctg gcagaatggc ttttccgaac ggctgatcgg
     1741 atcgatccga cgcgagtgtg cggaccacat cattgtcttt ggcgaggcgc acctgcgccg
     1801 gattctgcga tcgtatgctt gctactataa caggatcaga actcaccggt cattggacaa
     1861 ggatgcgccg atctctcgcc cggttcagcg gaccggcgtc attaattcac gcgccatcct
     1921 cggcggactc catcaccact acgctcgagt ttaggttttc ggtacagaca cccgataaaa
     1981 tcaggacaca tcggtctctg gacaaagcgc tgatctggcg gccagtacag cggatcggag
     2041 tcatcgcttc aaattcgata cttggtggac gtcagcaccg ctacctccaa acctaggttt
     2101 tcggcataca cgaaggctgg cgtgcgatcg ggacaggtat cgccgacact tttcagtttc
     2161 agatcgagat agttgtacct ttcatgcata tcgagacgta
//
LOCUS       DATMGX010000663         4500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_449293, whole genome shotgun sequence.
ACCESSION   DATMGX010000663 DATMGX010000000
VERSION     DATMGX010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449293"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VNR65_10790"
     CDS             <1..1009
                     /locus_tag="VNR65_10790"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HWJ19202.1"
                     /translation="KDESPPFSDPPIRAVPQEWVASLPGKLLVAANIMVARANSSLPN
                     LDDISARYFDGNTLVGSGVSSGAARAFTDFRIHGDGFSRILIQDLTLTPRQAGRLVQR
                     LEEIDSYRMMALLALPFAQELAPLLTSWETELVEVTSAMVRSGDKEEPVLLDRLTKLE
                     AEIESRRAASAYRFSAAAAYYDIVRRRIDELREERIQGLQLFQEFTERRLEPAMSTCS
                     ASTARQEALSTRVSRATNMLATKVDVTLEQQNQAVLASMDRRAKLQLRLQETVEGLSV
                     AAITYYIVGLVGYAAKAAHAGGFEVNPEIVMGISIPIVAGVIAYGLSRFRARLAAAEK
                     H"
     gene            1086..1661
                     /locus_tag="VNR65_10795"
     CDS             1086..1661
                     /locus_tag="VNR65_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HWJ19203.1"
                     /translation="MSVIFSLAAAALLLIGAIGLPAAIFIGLIVYSIGLIGMFGASAA
                     YNLVSHAGLKEILRRLDHAAIFVMIAGSYTPFALVVGGTAGYILLVAVWAIAALGVVM
                     KLCFPRRFDKLSVLLYLAQGWIVLFALDSIIAALPSRALTLLVAGGIVYTLGVPFHLM
                     ERLRFHNVIWHIFVLGGAACQFIAIRDAVMS"
     gene            complement(1770..2075)
                     /locus_tag="VNR65_10800"
     CDS             complement(1770..2075)
                     /locus_tag="VNR65_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HWJ19204.1"
                     /translation="MTETDKDQTREKFAVALQYELGTDAAPRVTAKGKGDVAERIVET
                     AKASGVHVEHNEPLAQSLSQLELDQQIPKELYRAVAEVIGFILRRAAVHDSPKQLRE"
     gene            complement(2095..3159)
                     /locus_tag="VNR65_10805"
     CDS             complement(2095..3159)
                     /locus_tag="VNR65_10805"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HWJ19205.1"
                     /translation="MLDQNPIALWTVDRVEPLALQLGRSGLQALLRNGDVVEARVIAM
                     LDKDVAQLEILGQKVEVSTPQALRAGSTISVAINRSGQSLELVIRPETNTSRPPSVPQ
                     TGSGSGRGSGVVAETIGSFLSVVASIEDRVLAAQAAINGAVLSSEANFQSANPGLAQP
                     SFNSVMQAYSAATLPSQVQLQAEVRARYELDSTPWPPHSAEDYDSGAPSSPYEASQLR
                     PQSALPSAPGQQSSDSALAIVVPFQLPQMPHPILMTIQQEDEDEAQPTSRSPAAKRWT
                     VNFSLDAGSVGQVNVTIALSAAAVSVRLSSDEAASATFLSTWLPELKTTLEEADFAVE
                     ELSVRGVRCSDTANNAPVLL"
     gene            complement(3545..3901)
                     /locus_tag="VNR65_10810"
     CDS             complement(3545..3901)
                     /locus_tag="VNR65_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19206.1"
                     /translation="MKVDVKIVPSRDGTGLLSSNETYELQLTIKFSDAEKATIENNTL
                     RDSLKLRRSDLNSGLQTRPWTDCVVREHEPANYDTFVAAQGAMVEFMAAVADILREEA
                     PKQNSASSSECPPRLQ"
     gene            4283..>4500
                     /locus_tag="VNR65_10815"
     CDS             4283..>4500
                     /locus_tag="VNR65_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19207.1"
                     /translation="MPDLLSRLISEETLCGVVVEYIRDDGLKPEIAAFGLSGFISDAC
                     ANEYLASPGPHFEFTLLDRALKQKPAFLS"
BASE COUNT          856 a         1304 c         1312 g         1028 t
ORIGIN      
        1 gaaggacgaa agcccgccgt tttccgatcc gccgatcagg gccgttccgc aggagtgggt
       61 cgcgagcttg ccgggcaagc tcctggtcgc ggccaatatc atggtcgcgc gggcaaactc
      121 gtcgcttccc aatctcgatg atatctccgc gagatatttc gatggtaata ccctggtcgg
      181 atcgggcgtt tcgagcggcg ccgcgcgcgc tttcaccgat tttcgcatcc atggcgatgg
      241 cttcagccgc atcctcattc aggacctcac gctgacgccc cgtcaggcgg gacgacttgt
      301 tcagcgcctg gaggagatcg acagctaccg aatgatggcg ctgcttgctt tgcccttcgc
      361 gcaggagctt gcgccgcttc tgacctcctg ggaaaccgag cttgtcgagg tcacctcagc
      421 aatggtgcgg tcgggtgaca aggaggagcc tgtgcttctc gatcgcctga caaagctcga
      481 agcggaaatc gaaagccgcc gtgccgcaag cgcctatcgt ttcagcgccg cagcggccta
      541 ttatgacatc gtgcggcggc gcatcgacga gcttcgggag gagcgcatac agggcttgca
      601 gcttttccaa gagtttaccg agcgccgtct tgagcctgca atgagcacct gctctgccag
      661 caccgcgcgc caggaagcgc tgtcgacccg ggtgtcgcgg gcgacgaata tgcttgcgac
      721 gaaggttgac gttacgcttg agcagcagaa ccaggctgtg cttgcctcga tggaccgccg
      781 cgcgaagttg cagcttcgct tgcaggaaac cgtcgagggg ctatcggttg ccgcgatcac
      841 ctattacatc gtcggtctcg tcggttacgc cgcgaaggcg gctcacgcag gcggcttcga
      901 ggtgaatccg gagatcgtca tgggcatcag cattccgatc gttgcgggcg ttatcgccta
      961 cggactaagc cgctttcgcg cccgcctcgc tgccgctgag aaacattaaa gcgggggacg
     1021 tcctgacgca cgatttcccg tcctatacgc gccttgagcg cattgctgat ggtagcgttc
     1081 atgcggtgag cgttattttc agcttagcgg cagccgccct ccttttgatt ggcgctattg
     1141 ggttgcccgc ggcgattttc atcggcctga tcgtctacag tatcgggctc attggcatgt
     1201 ttggggcatc ggcagcctac aatctggttt cgcatgccgg cctgaaggaa attcttcggc
     1261 gcctggacca tgccgcgatt ttcgtcatga tcgcgggcag ctatacgccc ttcgcgttgg
     1321 tcgtcggagg cacggcaggg tacatattgc ttgttgccgt gtgggcaatc gctgccctgg
     1381 gcgtggtcat gaagctttgc ttcccgagac gattcgataa gctttcggtt ctgctttacc
     1441 tagcccaagg ctggatcgtg ctgtttgcgc tcgattccat aattgccgca ctgccaagcc
     1501 gcgctcttac cctgctcgtg gcgggcggca tcgtttacac gctgggcgtc ccattccatc
     1561 tgatggagcg cctgcggttc cacaatgtaa tctggcacat cttcgtgctt ggtggcgcgg
     1621 cctgccagtt catcgcaata agagacgccg ttatgagtta acgccgcagc tacggcaagg
     1681 ttcatcagcc gcctgcgcat atgaggcggg cacctgcgga gatcaccgat ccgaagcgga
     1741 agccggcgcg atacggttgc ttgcttcgtt cactctcgaa gctgctttgg actgtcatgg
     1801 acggccgcac gcctcaagat gaagccgatc acctcggcca ccgccctgta gagttccttc
     1861 ggaatttgct gatcgagttc aagctgcgaa agcgattgcg ccaaaggttc gttatgctcg
     1921 acgtgaacgc ctgaggcttt ggctgtttct acaatcctct ccgcaacatc gcccttgccc
     1981 ttcgcggtca cccgcggggc ggcatccgtt cctagctcgt attgaagagc cacagcgaac
     2041 ttctcgcgcg tttgatcttt atccgtttca gtcaccgaag gcctcaatca aggattaaag
     2101 gagaaccggc gcattgtttg ctgtatccga gcagcggacc ccgcggaccg agagctcctc
     2161 gacagcgaaa tcggcctctt cgagggtggt tttcagctcc ggcagccaag tgctcaaaaa
     2221 ggtcgcggat gcagcctcgt cgcttgacag cctcacgctg acagcggccg cggatagcgc
     2281 gatggtcacg ttgacctggc cgacgctccc ggcgtcaagg gagaagttta cggtccaacg
     2341 ctttgctgct ggtgagcgtg acgtcggctg tgcctcgtct tcatcttcct gttggatcgt
     2401 catcaagatt gggtgcggca tttgcgggag ctgaaatggc accactatcg cgagagcaga
     2461 atcggaagat tgctgtccag gcgcggaagg gagcgctgac tgcggcctga gctgcgacgc
     2521 ttcgtaaggt gacgacggcg cgcccgaatc ataatcctcg gccgaatggg ggggccaggg
     2581 cgttgaatcc aattcatagc gcgcccgaac ttccgcctgc aattgcacct gagaaggcag
     2641 ggtggctgcg gaatacgcct gcatcactga gttgaagctt ggctgagcca agcccggatt
     2701 ggcgctttga aagttcgctt cgctgctcag gactgctcca ttgatcgcag cctgcgcggc
     2761 aagaacccga tcttcaatcg aggccaccac agatagaaaa ctgccgattg tttcggccac
     2821 gacaccggag ccccgaccgg agccgctccc ggtttgtggt actgatggcg ggcgagaagt
     2881 gttggtctcc ggtcggatga caagttcgag gctttggccg ctgcgattga ttgcaacgct
     2941 gatggtcgag cccgccctaa gcgcctgagg cgtgctgact tccactttct ggccgaggat
     3001 ctccagctgc gccacgtctt tgtcgagcat ggcaatgacc cgcgcctcaa ccacatctcc
     3061 gttccggagc agcgcctgca ggcccgaccg gcccagttgc aaggccaaag gctcgacacg
     3121 gtctacggtc cagagtgcga tggggttttg atcaagcaaa gcggtcgagc ctcagaaact
     3181 gcttcggaca cgttcactcg ttcgatgcta tcccggcaag caggagcgaa tcttgcgctt
     3241 agcagactct gaaaaatgtg cgtgcagatt gggtcgggct ggggatctcg tggatttttt
     3301 cgccggcgtg aagccgccct tggctgtgcg ggccaaacga gcgaaacatc tctgatctgc
     3361 gcgacgaacg gaagaatgca cccgttaagc cgctcctgcc gattgaaaag aagctgatcg
     3421 gctggagctt gggatcgggc tgacgctctt ggccgctctt ctcgccttga actccctcta
     3481 tcccaccaca gtgggagaat gatacgaggt gaaaaccacg acaggacaag ctgcttgcgg
     3541 gagattactg caagcgcggc gggcattcag acgatgatgc ggaattttgc ttcggtgctt
     3601 cctctcgcag aatatcggcc acggcggcca tgaactcgac cattgcaccc tgggctgcaa
     3661 cgaaggtatc ataattcgca ggttcgtgtt ctctaacaac acaatctgtc cacgggcggg
     3721 tctgaaggcc tgaattgaga tcgcttctcc tcagcttaag tgaatctcgc agagtattat
     3781 tctctatagt ggccttttcc gcgtcgctga atttaattgt cagctgcaat tcataagttt
     3841 cattggatga taaaagtccc gttccgtccc tgcttgggac gatttttaca tccactttca
     3901 tagcccactc caattctgga aaaccctaag gtccgccgtt ctgatggcgt ctcgtcattc
     3961 ctgccgcgat gaggttgtta ctatcgcagg gctgccggct tgagggtggc tttccccaca
     4021 gtggatagtg tttttatgta tcgctgggat acacagtgcc attacacaaa gctaaacccg
     4081 ccgtccccac cttgccgcgg catcctgcgc cccctatact cgacgaacaa caccgataaa
     4141 tggtggtaat tgcaaagatg gtgggggcac tgtggaggtt tcttttgttc gaagactcag
     4201 aacgatcaat gcgggcgacg cgccggaagc cgccgcattc atccagcgtt gttatccttt
     4261 tgcggatcaa cttggacact cattgcctga tcttctcagt cggttgattt cggaagagac
     4321 cttgtgcggc gtggtcgttg agtacatccg cgacgatggc ttaaagccgg agatcgcagc
     4381 ctttggcctc tcgggcttca tcagtgatgc ctgtgcgaac gagtacctcg cttcgccagg
     4441 tcctcacttc gaattcacct tgttggaccg cgctctcaag cagaaaccgg ccttcctatc
//
LOCUS       DATMGX010000664         3546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_164575, whole genome shotgun sequence.
ACCESSION   DATMGX010000664 DATMGX010000000
VERSION     DATMGX010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164575"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VNR65_10820"
     CDS             <1..258
                     /locus_tag="VNR65_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19208.1"
                     /translation="SIDKPGNRAKAFRVLAKLSFPGALLNDASRNGFGMPEAVPVSYL
                     VDAQGIVRDKFINVDEGLLDDVVTPLLKERPAGPIPAEMRQ"
     gene            255..764
                     /locus_tag="VNR65_10825"
     CDS             255..764
                     /locus_tag="VNR65_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19209.1"
                     /translation="MKVILKGVAFVMALLLLPISISGAIAGDWAVAGDGCKVWNPNPT
                     TGENIRWTGPCKDGIVEGMGTLEWQRSGKTYERDEGQWRGGHQISGSQVWPGGQYDGQ
                     FADSLPNGRGVLVFGDARYEGQFVNGKPNGKGTLTNASGSFDGNWADGCFNDGKHRAA
                     IGVSLQSCP"
     gene            804..959
                     /locus_tag="VNR65_10830"
     CDS             804..959
                     /locus_tag="VNR65_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19210.1"
                     /translation="MRTLFAFGLIGSVFAAAINVTPTSAEPRCRMEKQCRWVNFKKVC
                     TYVQVCR"
     gene            1188..1646
                     /locus_tag="VNR65_10835"
     CDS             1188..1646
                     /locus_tag="VNR65_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19211.1"
                     /translation="MTISRVSIVSFIALALTAIALIPAGAHVFEMSAKMKLGRDAYFV
                     VQGLYRGWAWFGIAIVGGLLACVANAALPGNRRDRILSLFAALMIAASLAIFFTYVYP
                     ANQSTQNWTTIPANWKNLRYQWEWGHVVSAVFTFLAFLALAWSAVTRKTA"
     gene            complement(1656..1958)
                     /locus_tag="VNR65_10840"
     CDS             complement(1656..1958)
                     /locus_tag="VNR65_10840"
                     /inference="COORDINATES: protein motif:HMM:NF018545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HWJ19212.1"
                     /translation="MSEMDPRIEIELEAAAFRRLLQHLRSRTDVQNIDMMNLAGFCRN
                     CLSNWYEEAAAERGIAVSKEEAREHVYGMPYAEWKARYQTETTPEAKAKFGETHGH"
     gene            complement(1921..2817)
                     /locus_tag="VNR65_10845"
     CDS             complement(1921..2817)
                     /locus_tag="VNR65_10845"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HWJ19213.1"
                     /translation="MQLISTPPEPQPGKCAGFEIFRRSPGNSQFLILCDHATNRLPPE
                     YGTLGLDEGQLKRHIAYDIGALAVALQIGRRLQATVISSCFSRLLIDPNRGEDDPTLI
                     MQISDGALVPGNVRVDEAERQKRISRFFVPYHAAIAEEIEAMLSRSVIPVIASIHSFT
                     ENWRGVPRKWEASVLWDKDPRLAVPLLRELRERTGFEIGDNEPYTGHLRNDSIFRHAT
                     VRGLPNALIEVRQDLIRDARGQREWAELLADCFAAILADPASGRQLSQVDYFGSHSDI
                     EEEHLKARTAHERNGPEDRDRA"
     gene            3088..>3546
                     /locus_tag="VNR65_10850"
     CDS             3088..>3546
                     /locus_tag="VNR65_10850"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HWJ19214.1"
                     /translation="MPLNTRRFLQTLFDAAVAAVLPAKCLPRHFPEAPADGKIIVIAC
                     GKAAAGMARAAEKHYGQQEKPLLTGVAITRYGFGEPLKSIGLIEAGHPVPDKASEYAA
                     AEAMKLARSAKPDDLILFLVSGGASALCAAPAGDVTLSEKQALTKALLNSG"
BASE COUNT          703 a         1032 c         1062 g          749 t
ORIGIN      
        1 agcatcgata agccggggaa tcgagccaaa gcctttagag tgctggcgaa gctctcattc
       61 ccaggggcgc tgttgaacga cgcgagccgc aacgggttcg gtatgcccga ggcggtgccg
      121 gtcagttatc tcgtggatgc tcaaggtatc gtgcgggaca aattcatcaa cgttgacgaa
      181 ggccttctag atgacgtggt caccccgctt ttgaaggaaa ggcctgccgg gccaatccca
      241 gcagagatgc gacaatgaaa gtcattctta aaggcgtggc cttcgtcatg gcgctcctgc
      301 tccttccgat ctccatctcg ggtgcaatcg caggcgattg ggccgtggca ggcgacggat
      361 gcaaagtctg gaatcctaat ccaacaaccg gtgaaaacat tcgctggacc ggcccttgca
      421 aggacgggat tgttgaaggt atgggcacgc tcgaatggca aagaagcggc aagacctacg
      481 agcgcgacga ggggcaatgg cgcggcgggc atcaaataag cggttcacaa gtgtggcccg
      541 gcggtcaata cgatggccag ttcgccgaca gcctccccaa tggccgcggc gtccttgttt
      601 ttggtgatgc tcgatacgaa ggccagtttg tgaacggcaa gccgaatgga aaagggacac
      661 tgaccaacgc gtccggctcg ttcgacggca attgggcgga tggttgtttc aatgatggga
      721 aacatcgcgc cgccattggc gtttcgttgc aatcctgccc ttagttttcc ctgagtgagt
      781 ttcaaatcct tgggagtcag atcatgcgaa cccttttcgc tttcggcctc atcggcagcg
      841 ttttcgcagc cgctattaac gtaacgccaa cgtctgccga gccgcgttgc cggatggaaa
      901 agcaatgccg ctgggtgaac ttcaagaaag tttgcaccta tgtgcaggtc tgccgataag
      961 agcgcctcag gcgggatcgc gcgcgcgttt actgggcaaa ggcacgctcc tggccccccc
     1021 ccgctgcagt gaccttggca tcgattgagc cggttttggc gaaggtggag caaagctccc
     1081 gatttgctcc aacccgccgg gaagcgggcc gagtctccat ttcgactcgt tttgcagcct
     1141 ataagcctcc gtgattctcc gctttggcga tgacaaggag aggcgtcatg acgatttccc
     1201 gcgtttccat tgtgtcgttt atcgctcttg cgctgacggc gatagcgctc ataccggcgg
     1261 gtgcgcatgt cttcgaaatg tcggcgaaga tgaaacttgg ccgggacgcc tacttcgtgg
     1321 tgcagggcct ttatcgcggc tgggcctggt tcgggattgc cattgtcggc ggtctcctgg
     1381 cttgcgtggc gaacgcagcc ttacccggca accgacgcga taggatcttg tcgctcttcg
     1441 ccgcgctcat gatcgccgcg tcgctggcga tcttcttcac atacgtctat cccgcgaacc
     1501 agagcacgca gaactggaca acaatccccg ccaactggaa aaacttgcgc tatcagtggg
     1561 agtgggggca cgtcgtgtcg gcagtattca ccttcctcgc attcctggct ttggcgtggt
     1621 ctgcggtaac gaggaagaca gcttaagccg cccgttcaat gcccatgcgt ctcgccaaat
     1681 ttcgccttcg cttccggggt cgtctcggtc tgatatcgag ccttccactc ggcgtagggc
     1741 atgccgtaaa cgtgctcgcg ggcctcttcc tttgaaaccg cgatgccccg ttccgcggcc
     1801 gcctcctcat accaattcga aaggcagttc cggcagaagc cggcaaggtt catcatgtcg
     1861 atattctgca cgtcggtgcg tgagcgaagg tgctgcagga gcctgcgaaa ggcagcggcc
     1921 tcaagctcga tctcgatcct cgggtccatt tcgctcatgt gctgtcctcg cctttaaatg
     1981 ttcctcctct atgtcggaat gcgatccgaa ataatccact tgggacaact gcctcccgga
     2041 ggcggggtcc gcgaggatgg cggcgaagca atcggcgaga agctcagccc actcccgctg
     2101 ccccctggcg tcgcgaataa ggtcttgccg gacttcgatc agcgcgttcg gcaggccgcg
     2161 aaccgtcgcg tggcggaata tgctgtcgtt gcgcaaatgc ccggtatagg gctcgttatc
     2221 gccgatctcg aacccggttc gttcgcggag ctctcgcagg agagggactg cgaggcgcgg
     2281 gtctttgtcc cacagcaccg aagcctccca cttcctcggg acaccacgcc aattctcggt
     2341 gaagctgtgg atcgaggcga tgaccgggat cacgctccgg ctaagcatgg cttcgatttc
     2401 ttcggcgatg gcggcgtgat agggcacaaa gaaccgtgaa atgcgctttt gccgctccgc
     2461 ttcatcgacg cgcacattac ccgggacgag cgcgccgtcc gaaatctgca tgatgagcgt
     2521 cgggtcgtcc tcaccgcgat tgggatcgat gagaaggcga gagaagcacg aagagatcac
     2581 agtagcctgc aaacggcgcc cgatttgcag cgcgacggcg agagcgccaa tatcgtacgc
     2641 gatgtgccgc tttagctgcc cttcgtcgag gcccagcgtc ccatattcgg gtggcaacct
     2701 gttcgtggcg tgatcgcata gaataaggaa ctgggagttg ccgggcgatc ggcggaaaat
     2761 ctcgaagccc gcgcatttcc cgggctgcgg ctccggtgga gtggatatca gttgcatcga
     2821 tgcatcattt agtggggaca gtcggagttt tggagaaata cggcgcacag ctcccgcatg
     2881 agtgatcatg gctcgttggc tggcgtcaca tgcgatgtta atgccacctc tgggtgtgga
     2941 agttcaagcc ttttggccaa gcctcccccg taaacacaaa gccgagccat actttttggt
     3001 gagcgactta attccaatat cagccgttcc agagacgcag tagttttccg aaatgctgaa
     3061 gccgggccaa agcagcgagt gtttgccgtg ccgctaaaca ccagacgctt tctccaaacc
     3121 cttttcgacg ccgccgtcgc ggctgtcctg cccgcaaaat gcctgccacg ccacttcccg
     3181 gaagctcccg cggatgggaa aatcattgtc atcgcctgcg gtaaggcggc ggcgggcatg
     3241 gccagggccg cggaaaaaca ctatgggcaa caagaaaagc ctctcctgac gggcgtcgcc
     3301 atcacgcggt acgggttcgg cgagccgttg aaatcgatcg ggcttatcga ggcgggccat
     3361 cccgttcctg acaaggcgag cgaatatgcg gcagccgaag ccatgaagct ggcgcgctcg
     3421 gcaaagcccg acgatctcat tcttttcctg gtgtccggcg gcgcttctgc gctttgcgcg
     3481 gcgcctgcgg gcgatgtcac cctctcagag aaacaagctc tcacaaaggc gcttctcaat
     3541 tccgga
//
LOCUS       DATMGX010000665         3643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_182346, whole genome shotgun sequence.
ACCESSION   DATMGX010000665 DATMGX010000000
VERSION     DATMGX010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182346"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(611..1213)
                     /locus_tag="VNR65_10855"
     CDS             complement(611..1213)
                     /locus_tag="VNR65_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19215.1"
                     /translation="MRDLLPATFKMLSFCCLAMAVNGCAGPSMSGRPENSYTPVITEE
                     SMLTAARANSDGPIEAAASNGCPLVQVEGGQRSVTIYERNRVGDGASVVHRAEITKTA
                     RECQATMGVVQVKYGIAGRVLLGPKGKPGTVTLPAVMQVFDRAKNQVKSDPVSVTVTI
                     TRENPLSYFSIVRDVTIPVKDGTTPQDYTISLAFEKKGAA"
     gene            1465..2427
                     /gene="ispH"
                     /locus_tag="VNR65_10860"
                     /gene_synonym="lytB"
     CDS             1465..2427
                     /gene="ispH"
                     /locus_tag="VNR65_10860"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002190.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWJ19216.1"
                     /translation="MTTKPHLDVLLAAPRGFCAGVDRAIQIVEMAIEKFGAPVYVRHE
                     IVHNRTVVESLEAKGAVFVEELSEVPNTDAPVIFSAHGVPKSVPAEAAKRNFFYLDAT
                     CPLVSKVHVEAERHFEAGLEIILIGHSGHPEVIGTLGQLPEGAVTLIEKVQDVEAFTP
                     RDPSRLAYITQTTLSIDDTIEMVKALKARFPLIQGPHKDDICYATTNRQRAVKAIADK
                     CDAILVVGAPNSSNSKRLVEVAFRSGCPKAMLVQKASEIPWDNLHHIKTLGLTAGASA
                     PEILVEEIIEQFKARYDVTVEIVRTATEDVIFKIPRALQQAAAQ"
     gene            2439..3398
                     /locus_tag="VNR65_10865"
     CDS             2439..3398
                     /locus_tag="VNR65_10865"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HWJ19217.1"
                     /translation="MAVYTEVTAEDLYALLERYDIGSLTAFKGIAEGVENSNFFLGTG
                     TGQFILTLYERRVSAHDLPFFLGLMEHVAQSGVRCPTPVHDREGRVLQVLAGKPAAIV
                     TFLPGYSLQKPKPAHCRALGAVLAALHEAGLSFPLKRENALSLSGWNKLYVSVGGAAD
                     KIAPGMYAQLEHVLNALSEKWPEDLPRGVIHADLFPDNVLFLNDAVSGVIDFYFACND
                     LLAYDLAICMNAWCFELDGAFNVTKAKALFNGYNSVRRLQPEEISALPILARGAAVRF
                     FLTRLYDWFNTPDTAFVKRKDPMEYWRKLTFHQGVETPRAYGL"
     gene            3388..>3643
                     /locus_tag="VNR65_10870"
     CDS             3388..>3643
                     /locus_tag="VNR65_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HWJ19218.1"
                     /translation="MASDNASEQESTVTIYTDGGCKGNPGPGGWGAILIYKGREREIY
                     GGEPLTTNNRMELMAAISALEALKRGCPVDLHTDSQYLRNG"
BASE COUNT          762 a         1111 c         1008 g          762 t
ORIGIN      
        1 cttgcccgca gacaagccct tgcttgcatg gtctgcattc cgctgacagt catcccctcg
       61 ccccccactc aattgcccaa actcaaggca cggctcgaga tgccctatca aagagcgcag
      121 cgagcgagtt aggtaaaacc cgactcaata cgccgtatta tgttgtagct aacccagctt
      181 tggcgatcaa cgcgcaagct cgtacttgac cacactgaaa ccgttgcctg tgccttttga
      241 gcaccagaaa agcgtcttcc tgccgtagtt tagccacaag agagccaagc ttgagcgctg
      301 ccgacaactg tcgagcctcg aacaagcttc gaactgcaga attacctcca acttcgtact
      361 gaatcattaa aattggcgac acttagataa cggagcgagc gaaagggtat tcccggccag
      421 cttgctcgca cccaatccca ttcctgagca aaacggcgtc cgcagtgagg cgatgacggg
      481 aacgttacag cattgccgct gcgcaggagg ccggtctccg atcgcaatcg atgaagcctt
      541 gcgccgacaa cgctgaaagc gcctgtccga acgccggggc gcagctatca ggagcttgat
      601 cgaccgcctt ctaagcggcg cccttcttct cgaaggcgag ggaaatggtg taatcctggg
      661 gtgtcgtgcc gtcctttacg ggaatggtca cgtcgcggac aatcgagaaa taggagagcg
      721 gattttctct cgtgatcgta acggtgacgg agaccggatc ggacttcacc tggttcttgg
      781 ccctgtcgaa aacctgcatc acagccggca gagtgaccgt tccgggcttg cctttcggcc
      841 cgagcaggac gcgccccgcg atgccgtact tgacctgaac cacgcccatc gtggcctggc
      901 attcgcgtgc cgtcttggta atctccgccc gatgaacaac ggaagcccca tcgccaacgc
      961 gattgcgctc gtagatggtg accgagcgct ggccgccttc aacctgaacc agcggacaac
     1021 cattagaggc cgccgcttcg atcggaccgt ccgagttggc gcgcgccgcc gtaagcatgc
     1081 tttcctcggt gatcaccgga gtatacgaat tctcgggtct cccactcatc gaaggtccgg
     1141 cgcagccatt tacggccatc gcgagacaac agaagctcag cattttgaag gttgccggta
     1201 acagatccct catttgcccc ttcattttcc aacaccaaga tttcgatgct ttcaaagccg
     1261 ccgcgacttt gagtagcgtt gcttttttat ggtttgacgc gctcattatt gttacaagaa
     1321 ggcactggat ttgaatgcgc tcggggaccc tagcgggccg cggaaagggc cgcaagactg
     1381 gacgctcccc ttccctgcca aattggacct ttttgcggag ttccgcacgc cttttgcgat
     1441 tgctaagtct cggagatggc ccctatgacg acaaaaccac acctggatgt gttgctcgcc
     1501 gcgccaaggg gattttgtgc gggcgtagac cgagcgatcc agatcgtgga aatggctatc
     1561 gagaagtttg gggctcctgt ctacgtccgc catgaaatcg ttcacaaccg taccgttgtc
     1621 gaaagcctgg aagcaaaagg agcggttttc gtcgaggaac tgagcgaggt cccgaatacc
     1681 gatgcgcctg taatcttctc ggcacatggc gttccgaaat cggtcccggc ggaagcggcg
     1741 aagcggaatt tcttttatct cgacgcgact tgtccgctcg tgtcgaaggt tcacgtcgag
     1801 gccgagcgcc atttcgaagc cggactcgag atcatcctga tcggccactc cggccatccc
     1861 gaggtcatcg gcactctggg ccagcttccc gagggcgcgg tgacgctcat cgaaaaagtg
     1921 caggatgtcg aagccttcac gccgcgcgat ccttccagac ttgcctatat aacgcagacg
     1981 acgctgtcga tcgacgacac catcgagatg gtcaaggcct tgaaggcgcg cttccccctc
     2041 atccagggtc cgcacaagga cgacatctgt tacgcgacga caaaccggca gcgcgccgtg
     2101 aaggcgattg ctgacaaatg cgacgccatt cttgtcgtcg gcgctcccaa cagctccaac
     2161 tccaagcggc tcgtggaagt cgctttccgg tccggttgcc cgaaagccat gcttgtgcaa
     2221 aaggcttcgg aaattccgtg ggataacctc caccacatca aaacattggg actcaccgcc
     2281 ggcgcctcag cccccgaaat cctcgtggag gagatcatcg agcagttcaa ggcgcgctac
     2341 gacgtgacgg tcgagattgt gaggaccgcg accgaggatg tgatcttcaa gattccgcgg
     2401 gccttgcaac aggccgcggc gcagtaagcc gccgaagcat ggcggtctac accgaagtca
     2461 cggccgagga tctgtacgcg ctactcgagc gctacgatat cggctcgctt accgccttca
     2521 aggggatcgc ggaaggcgtc gagaacagca acttctttct tggcaccggt accggtcagt
     2581 tcatcctgac cctgtatgag agacgcgtct cggcgcacga tcttcctttc tttctcggcc
     2641 tcatggaaca cgtcgctcaa agcggcgtca ggtgccccac acccgttcac gaccgcgagg
     2701 ggcgcgttct tcaagtgctt gcgggcaagc ccgctgctat tgtcacattc ctgcccggat
     2761 attccttgca gaagccgaag ccggcgcatt gccgcgcgct tggcgcggtg ctcgccgcgc
     2821 ttcatgaagc aggcctgtcc ttccctctga agcgcgaaaa cgcgctctcc ctttcgggct
     2881 ggaacaagct gtacgtctcc gttggcggcg ccgcagacaa gatcgcgccg ggtatgtacg
     2941 ctcagctgga gcatgtcctg aatgcgttgt ctgaaaaatg gcccgaagat cttccgcgcg
     3001 gggtcattca cgcggatctc tttcccgaca atgtcctgtt cttgaacgac gccgtttccg
     3061 gcgtgatcga cttttatttc gcttgcaacg atcttcttgc ttatgatctc gcgatctgta
     3121 tgaacgcctg gtgtttcgaa ctcgatggtg ctttcaacgt gacgaaggcg aaggcgctct
     3181 ttaatggtta taattccgtc cgccgccttc agccggagga gatttccgcc ctgcccattc
     3241 tcgcgcgcgg cgcggcggtt cgctttttcc tgacccgcct ctacgactgg ttcaatactc
     3301 ccgacaccgc cttcgtaaaa cgcaaagatc ccatggaata ttggcggaag ctcaccttcc
     3361 atcagggcgt ggagacgcct cgcgcctatg gcctctgaca acgcgagcga gcaggaaagc
     3421 accgtcacaa tctacactga cggcggctgc aaaggcaatc cgggccccgg aggctggggc
     3481 gctatcttga tctacaaggg gcgcgagcgc gagatctatg gcggggaacc gcttacgacc
     3541 aacaatcgaa tggaactgat ggcggcaatt tccgctctcg aagcgctgaa acggggctgc
     3601 ccggtcgacc tgcacacgga ctcacaatat ttgcgaaacg gca
//
LOCUS       DATMGX010000666         4591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_333063, whole genome shotgun sequence.
ACCESSION   DATMGX010000666 DATMGX010000000
VERSION     DATMGX010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333063"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VNR65_10875"
     CDS             complement(<1..258)
                     /locus_tag="VNR65_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWJ19219.1"
                     /translation="MTGKAKRGAAAHQRSPTGAQRVYEALRYKIIALDVAPGAALEEE
                     QLCRYYKVSRTPVREALIRLASEDLVELQPNRGAKVASLQFV"
     gene            442..1797
                     /locus_tag="VNR65_10880"
     CDS             442..1797
                     /locus_tag="VNR65_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ19220.1"
                     /translation="MSEEMISARVERLPVARWYVRTMLIVGAAGFFDAFDALTIAFVV
                     PVLIGIWHLAPQEIGPKIGLLVSIGYVGQLIGAIFLSGLAEKYGRLSVLRWSVAIIGL
                     LSVACAFSRSYESLFWLRFVQGLGLGAEVPIAATYMNEISKAEMRGRLVVLFQAIFAF
                     GVMVTAFVAIWVVPHLGWEWMFIIGAVPALLAIWLRRLVPESPRWLANRGRLAEADVI
                     LSTIEAEVTSSGMTLPPLPADIPPIKKERVHWTELFSKDYLGRTITAWFIAFATSFLG
                     YGLLVWLPTLYRTVYHLDVSQTLQYAFISYFIGMFGAASGALVIDRFGRKPCFIISFI
                     GAGIPLLALWMIGIPPVKTVVVLASVALFFISILLAAIYVYMPEIYPTRMRALGAGTA
                     SAWFRFASIVGPPTVGAILSSAGSGTVFLVFGATGIVGSIIVLFFAVEPRGQVLEAIA
                     Q"
     gene            1835..2833
                     /locus_tag="VNR65_10885"
     CDS             1835..2833
                     /locus_tag="VNR65_10885"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWJ19221.1"
                     /translation="MTTRGIGDHRYIAVLLPYDDRLKIDEQAYRRFIRYFLKEPRFLK
                     DGGLCINPEAGEIFYLTRQEKRRVLEIAMEEAKGKVPIIAGTWAITTDEMVDTAKEAK
                     AMGVDGIFVTPPGGAQDVTSCWDADAYPEIWGDQIKAQDRAVDLPIVTHPVGGARPPF
                     YPGLPLGAAMTLCREIPNIVGWKMTYTYEGFRLIAKGLRSLPRHVAIMGALASNFHEY
                     RATGNFDGTLSGFWNFAFEPMLDHLEAWDKMDVVRATQIWNSGLAELHSYIADMGRLH
                     IRYKTATWLRGLIPSPFMRPPMPQPKQVEIDTIYGLLKKLGLPVIELNDTKLELAA"
     gene            2916..4079
                     /gene="pyrD"
                     /locus_tag="VNR65_10890"
     CDS             2916..4079
                     /gene="pyrD"
                     /locus_tag="VNR65_10890"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HWJ19222.1"
                     /translation="MGLYRWVVRPLLFHLDAERSHSATLAACQRFGDLALVRTVLARK
                     FGCRDSRLRIAVAGIPFPSPVGLAAGFDKNAYALEVTSRLGFGALEIGSVSEHPSAGN
                     LGRPRVWRLELDQSLRIHYGCPNDGAEAIAARMRRHRIAIPLGINLVETNTGVTSSAA
                     HVITELSQAISHFIGIADYITLNLSCPNMPQGSQGLFDDPAMLTLLLEECARHDELPP
                     IFLKVAPPGNPEDPRVIDTILEAVDPFAFVKGFILNIHNRKPHAMLRTPAAKLDRMRG
                     QITGPSLRGPVNAAIRSWYARIDRARHALIGVGGIASAEDAYETIRLGASLVQLYTAL
                     VYKGPGLIKCINEGLCHLLDRDGLRSIRDAVGLDNGGNAKRGYAAARLATAKA"
     gene            4081..4584
                     /locus_tag="VNR65_10895"
     CDS             4081..4584
                     /locus_tag="VNR65_10895"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HWJ19223.1"
                     /translation="MTATKLDHPSKWWDRLFAPSSCLAVITTVDRAGQINAASYGTCT
                     RVKHEPVYIAFTANIGSDTAQNVLDTSEFVVNLPRFDKESLERVRIVGLPFAHGVNEL
                     EKAGFTALPSTVVKPPAFWNAHGTLSAGSNGPRSGQAGLWSLASSSRLRSTPIALTRK
                     DMFFGNK"
BASE COUNT          917 a         1336 c         1330 g         1008 t
ORIGIN      
        1 gacgaattgg agcgacgcca ccttggcacc acgattcggc tggagctcaa ccaggtcttc
       61 cgaggcgagg cggatcaacg cctcgcgcac cggcgttcgc gataccttat aatatcggca
      121 gagctgctcc tcctcgagcg cggcacctgg agcaacgtca agcgcgatga tcttataacg
      181 cagggcctcg taaacgcgct gcgctccggt cgggctgcgc tggtgcgccg ccgcgccgcg
      241 tttggctttt cctgtcattc acatatccac actcaaatcg cattatttat atgtattctt
      301 atagtataca tcgttatcga catccaccag agagcttgct aacttatcgg tgccgaccgg
      361 agtccaaaaa tacggcggcc atgcccaggg tggaaacgaa tgcagaccat gacatcgaag
      421 gctcaatcgt ccgcggcggc attgtccgag gaaatgatat cggcgcgggt cgagcggctg
      481 cccgttgcac gctggtacgt acgcaccatg ctgatcgtcg gcgcggcggg gtttttcgac
      541 gccttcgatg cactgacaat cgctttcgtg gtcccggtgc taatcggtat ctggcatctg
      601 gcgccacagg aaattggacc gaagattggg cttctggtct cgatcggcta tgtgggacag
      661 ttgatcggcg cgatttttct gagcggactc gccgagaaat acgggcgact cagcgtactg
      721 cgctggagcg tagccatcat cggtctgctc agtgtcgcct gcgccttctc acgaagctat
      781 gagtcgctct tctggctccg tttcgtccag ggtctcgggc tcggcgccga agtgccgatc
      841 gcggcgacct acatgaacga aatcagcaaa gccgaaatgc gcggccggct ggtggtgttg
      901 ttccaggcta tcttcgcgtt cggcgtgatg gtgacagcct ttgttgcgat ctgggtcgtg
      961 ccccatttgg gttgggaatg gatgttcatc atcggcgccg taccggcctt gctcgcgatt
     1021 tggctgcgcc gactcgtgcc ggaatcgcct cgttggctcg ctaatcgcgg cagattagcc
     1081 gaggccgacg tgatcctgtc gacgattgag gccgaagtca cgtccagtgg catgacgctg
     1141 ccaccgctac cggccgacat tccgccaatc aaaaaagagc gtgtccattg gactgagctt
     1201 ttctccaagg attatctcgg gcgcacgatc acggcttggt ttatcgcctt cgcgacctcg
     1261 ttcctcggct atggcctgtt ggtctggctg ccaacgctct accgtaccgt ctaccacctc
     1321 gacgtcagcc aaacattgca atatgccttc atcagctatt tcataggcat gttcggcgcc
     1381 gcttccgggg cgctggtgat cgatcgcttc ggccgcaagc cctgctttat catctcgttc
     1441 atcggcgcgg gcattccgct gttggcgctg tggatgattg ggatcccgcc ggtcaagacc
     1501 gtcgtcgtgc tggcctccgt cgccctgttc ttcatttcca tcctgctcgc cgcaatctac
     1561 gtctatatgc cggaaatcta ccccacgcgc atgcgcgcgc ttggcgccgg cacggcgagc
     1621 gcatggttcc gttttgcatc gattgtcggc ccaccgacgg tcggcgcgat cctcagcagt
     1681 gctgggagcg gcacggtatt cctggttttt ggtgcaaccg gcattgtcgg ctctatcatc
     1741 gtgctattct tcgccgttga gccgcgcggc caggtgctcg aggcaattgc gcaatgagtg
     1801 caagcaaaac ttttcgattt gcaaggagac tcccatgacc actagaggaa tcggcgacca
     1861 ccgttacatc gcagtgctgc tgccttatga tgataggctc aaaattgacg aacaagccta
     1921 tcgtcggttc atccgctatt tcctcaagga gccccgcttt ctcaaggatg gcggattgtg
     1981 cattaatccc gaggccggtg aaatttttta cctgacgcgg caggaaaagc ggcgcgttct
     2041 agagatcgcg atggaggagg caaaaggcaa ggtgccgatc atcgccggca cctgggcgat
     2101 taccaccgac gagatggtgg acaccgccaa ggaggccaag gccatgggcg tcgatggaat
     2161 cttcgtgaca ccgccgggtg gcgcgcagga tgtgacttcg tgctgggacg ccgacgccta
     2221 ccccgagatt tggggcgacc agatcaaggc gcaagaccgc gccgtcgacc tgccaatcgt
     2281 cacccatccg gtaggcggcg ctcggccacc attctaccca gggctaccat tgggcgccgc
     2341 catgactttg tgtcgggaaa tcccgaacat cgtcggctgg aaaatgacat acacctacga
     2401 aggattccgc cttatcgcca agggattgcg ctcgctgccg cgccatgttg caatcatggg
     2461 tgcgctcgca tccaacttcc atgaataccg cgccacgggc aatttcgacg gcacgctatc
     2521 aggcttctgg aacttcgctt tcgagccgat gctcgatcac cttgaggcct gggacaaaat
     2581 ggacgtcgtc cgcgcgacgc aaatctggaa cagcgggctg gccgagctcc acagctacat
     2641 cgccgacatg ggccggctcc atatccgcta caagaccgcg acctggttgc gaggcctgat
     2701 tccgagccca ttcatgcgac cgccgatgcc ccaaccgaaa caggttgaaa tcgacacaat
     2761 ctatggcctg ctcaagaaac tcggcctgcc tgtgatcgag cttaacgata cgaaacttga
     2821 gctcgcggcc tgaggagtag cggccgcgac catagcgaag acaccgataa gatacgtgtg
     2881 gccgctaaga ccatccaacc acggatttgg ggtaaatggg cctttatcgc tgggtcgttc
     2941 gtccgcttct ctttcatctc gatgctgaac ggagccatag cgcgaccttg gctgcgtgcc
     3001 agagattcgg agacctagcg ctcgtacgaa ctgtgttggc gcgcaaattc ggctgcagag
     3061 attcgcgcct acggatcgct gtagccggga ttccgtttcc cagtccggtt ggtctcgctg
     3121 ctgggttcga caagaacgca tatgcgctcg aagtcacatc gaggcttggg tttggtgctc
     3181 tcgaaatcgg gtcggtttcg gagcatccgt cggccggcaa ccttgggagg cctcgggtct
     3241 ggcggttgga gctcgaccag agccttcgga ttcattatgg ctgtcctaac gacggggcgg
     3301 aagcaatcgc agcccgcatg cgccgtcacc gaatcgctat accgctcggc atcaaccttg
     3361 ttgagacgaa tactggcgtt acgtcatcgg ctgcccatgt cattacggag ctgagccaag
     3421 cgataagcca cttcattggg atcgcggatt acataactct caatctaagc tgcccgaaca
     3481 tgccgcaagg cagccaaggg ctctttgacg atcctgcgat gctcacgttg ctcctggaag
     3541 aatgcgcgcg gcacgatgaa ttaccgccta tcttcttgaa ggttgcgccg cccggaaacc
     3601 cggaggatcc gcgggttatt gatacgattc tcgaagcggt ggatcccttc gcgtttgtga
     3661 aaggattcat cctgaacatc cataatcgaa agccccacgc gatgctgcgt acgcctgccg
     3721 cgaaacttga ccgtatgcgc ggccagatca cagggccttc gctacgcggg ccagttaatg
     3781 ccgctattcg cagctggtac gctcgaatcg accgcgcccg gcacgccctg attggggtgg
     3841 gcggcattgc atctgccgaa gatgcttatg aaaccatccg gctgggagcc tcgcttgtgc
     3901 aactctacac agcgctggtc tacaaaggac cgggtttgat caaatgcatc aacgagggat
     3961 tgtgccatct gctcgatcgg gatggactgc ggagcatccg cgacgcggta ggtttggaca
     4021 atgggggcaa tgcgaagcgc ggttatgcag ctgcgcggct tgccactgcg aaagcttaaa
     4081 atgacagcga caaaattaga tcaccctagc aaatggtggg atcgactatt tgccccaagc
     4141 tcatgccttg cggtgataac gaccgtggat cgtgctggcc agataaacgc ggcgtcttac
     4201 ggaacctgca cacgggtcaa acacgagccg gtctatattg catttaccgc taatatcggg
     4261 agcgataccg cccagaatgt tttggacacg agtgaatttg ttgtcaattt accccgcttt
     4321 gacaaggaaa gccttgagcg cgtacggatc gttggcttac cctttgcaca cggcgtgaac
     4381 gaactcgaga aggcgggctt tactgccctg ccctccactg tcgtaaagcc ccccgcattc
     4441 tggaatgccc acggcacttt gagtgcaggc tcgaatggac caaggagtgg gcaggccggc
     4501 ttatggtcgt tggcgagctc gtcgcggctt cggtcgacgc cgattgcgtt gacgagaaag
     4561 gatatgttct ttggcaacaa gtaagatctg c
//
LOCUS       DATMGX010000667         2028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_323354, whole genome shotgun sequence.
ACCESSION   DATMGX010000667 DATMGX010000000
VERSION     DATMGX010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323354"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VNR65_10900"
     CDS             complement(<1..815)
                     /locus_tag="VNR65_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWJ19224.1"
                     /translation="MTEFSKLVPAHVQKLASYKPGKSLRQAELESGMRCIKMASNENP
                     FGPSPLAIEAIKAAAREVNFYPDNGVTELTARLAEIHEIEPAQILVTAGSSALLDIMA
                     RTLLGPGLNAITSKLSFIVYPIVTRATGAQFIEVPTVNDGYDLDRILAAVDVNTRLIY
                     LSNPNNPTGTILDANAIDRFLDRLPPHVITVLDEAYYDYASYFAAERGFDYSHALDYV
                     RQERRVVVLRTFSKAHGLAGVRVGYGMGPAELMGYFARRKSAFMVSSLGEAAAL"
     gene            complement(870..1691)
                     /locus_tag="VNR65_10905"
     CDS             complement(870..1691)
                     /locus_tag="VNR65_10905"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWJ19225.1"
                     /translation="MPAERLQKIIAAAGIASRRKAEELITSGLVSVNGQIVTELGSKA
                     DIELDHIKVNGKLLKGPERPVYLLLNKPKGYVTTVTDPEGRPTVLDLVRGVGARIYPV
                     GRLDYLSEGLLLLTNDGELAQKLMHASSHVAKTYLVKIAGKATEEQIDKLRAGIMLPA
                     ERKSLKTPADRVPKTGWKRKSTAVKTAPVRIKLVRDAANPWYEVTLVEGRNRQIRRMF
                     EQIEHHVEKIKRVRYGPLVLDVEPGEFRHLTAQEVAQLKNPTAAPPRTHNRDRAR"
     gene            complement(1696..>2028)
                     /locus_tag="VNR65_10910"
     CDS             complement(1696..>2028)
                     /locus_tag="VNR65_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19226.1"
                     /translation="KQVIGRPILYKTTKEFLLRFGLNDVSELPSMEEFEKLGDPQADL
                     FAAAGTEKAEPSAERTLEVSGADESEPVTEAVPEDSNANSLVGDSAMPADGTYATEAE
                     AGSSPEKP"
BASE COUNT          363 a          611 c          599 g          455 t
ORIGIN      
        1 agcgccgcag cctcgcccag cgaggagacc atgaatgccg atttccggcg cgcgaaatag
       61 cccatcaact ccgccgggcc catcccataa ccaaccctca ccccagccaa gccgtgagcc
      121 ttggagaaag tgcgcagcac cacgacccgg cgttcctggc gcacataatc cagcgcgtgc
      181 gaatagtcga aaccgcgctc cgccgcgaag tagctggcgt aatcgtaata agcttcatcc
      241 agcaccgtga tgacgtgcgg tggcagccga tcgaggaaac ggtcaatcgc gttcgcgtcc
      301 agaattgttc ccgtgggatt gttcgggttc gagaggtaga tcagacgggt attgacgtca
      361 accgccgcga gaatgcgatc aagatcgtag ccatcgttca cggttggcac ttcgatgaat
      421 tgcgcgccag ttgcacgcgt aacgatggga taaacgatga atgagagttt gctggtaatg
      481 gcgttcagcc caggccctag cagcgtccgc gccatgatgt cgagcagggc gctggaaccc
      541 gccgtcacca ggatctgcgc cggctcgatc tcgtggatct ccgccagacg tgcggtcagc
      601 tcggtaacgc cgttgtccgg ataaaaattg acctcgcggg cagccgcctt gatcgcttca
      661 attgccagag gagagggccc gaaggggttc tcgttcgaag ccatcttgat acaacgcatg
      721 ccgctctcca attccgcctg ccgcagcgat ttgccgggct tatagctggc cagcttttgc
      781 acgtgcgccg ggactagctt gctaaattct gtcattctat cctcaattat cttggcgact
      841 gatggcgctc gtcgcaaagc ggcggtccgc taccgggcac gatcgcgatt gtgcgtccgg
      901 ggcggagcgg ctgtcggatt tttcagttgc gccacttcct gcgccgttag atgccgaaac
      961 tcgcccggtt cgacgtccaa caccagcggc ccgtagcgca cccgcttgat tttttccacg
     1021 tggtgctcga tttgttcgaa catccggcga atctgccggt tgcgtccttc caccagcgtg
     1081 acctcgtacc agggattggc ggcgtcacgt accagcttta ttcgcaccgg cgcggtcttt
     1141 accgctgtgg acttccgctt ccagcctgtt tttggcacgc ggtccgcagg ggtcttgagc
     1201 gacttacgtt ccgcgggaag catgatgcct gcgcgcaatt tgtcgatttg ctcctcggtg
     1261 gccttgccag cgatcttgac caggtatgtc ttcgccacgt gcgacgaggc atgcatcagc
     1321 ttctgcgcca gctcgccgtc gttggtcagc agcagcaatc cttcgctcag gtaatcgaga
     1381 cgaccgaccg ggtaaatgcg cgctccaaca ccccgtacta aatctaatac cgtaggacgg
     1441 ccttccgggt cagtgaccgt ggtcacgtag cccttaggtt tgttgagcag caggtagacg
     1501 gggcgctccg gccctttcag cagtttgccg ttgaccttga tgtggtcgag ttcgatatcg
     1561 gccttgctgc ccagttcggt gacgatctgt ccattgaccg acaccagacc gctggtgata
     1621 agttcctcgg ccttgcgccg cgaagcgatt ccggcggcgg cgatgatctt ctgtagtcgt
     1681 tcagcaggca tgcggctacg gcttctccgg cgaacttccg gcttcggctt cggtggcgta
     1741 ggttccgtcg gcgggcattg cactgtcgcc tacgagggag ttggcattgg agtcttcagg
     1801 aacagcctcg gtcaccggtt ccgattcatc tgcccctgaa acttcgagtg tacgctcggc
     1861 gctgggttcg gctttttccg taccggccgc ggcgaacaag tcagcttgcg gatcgccgag
     1921 cttctcgaac tcttccatgc tgggtagctc ggagacgtcg ttgagcccga agcggagcag
     1981 aaattctttg gttgtcttgt agagaatcgg ccggccgatg acctgctt
//
LOCUS       DATMGX010000668         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_38253, whole genome shotgun sequence.
ACCESSION   DATMGX010000668 DATMGX010000000
VERSION     DATMGX010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38253"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1845)
                     /locus_tag="VNR65_10915"
     CDS             complement(301..1845)
                     /locus_tag="VNR65_10915"
                     /inference="COORDINATES: protein motif:HMM:NF008737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HWJ19227.1"
                     /translation="MTTTAAEDRLLFKGADWDFDTLARIYDGVAEIGLGEMGLNVYRP
                     RIEVITTEQMLDAYASTGMPLFYRHWSFGKQFVRNETLYRKGWQGLAYEIVINSNPCV
                     VYLLEENTATMQTLVLAHAAFGHNHFFRNNYVFKQWTDADGILDYLSFAKNFITACED
                     KYGAAEVERVLDAAHALQNQGVHRYPGKRQMDLRSEEKRERERREHGERLFNDLWRTV
                     PEQAKKAKRRSERDRRRALLGLPEENVLYFLEKTSPRLKGWQREILRIVRLIAQYFYP
                     QRQTKMMNEGCATYVHYQIMQRLLDEGRITEGSFLEFLHSHTSVVMQPDFDDRRYGGL
                     NPYALGFAMMKDIERICVDPTQEDRHWFPEMAGNGDAMGTLKDIWANYRDESFVSQFL
                     SPHVIRKMGLFLVVDDHREKDLNVGAIHNERGYNRVRRALARHYDVSRHDPDIQIVDV
                     DLEGDRRLELRHHVVDGVTLEEDDAERVLQHLANLWGYEVHLVETNDQGDLATKHHAE
                     PIHPFV"
     gene            complement(1845..>2510)
                     /locus_tag="VNR65_10920"
     CDS             complement(1845..>2510)
                     /locus_tag="VNR65_10920"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HWJ19228.1"
                     /translation="RLAKRSRDIPFIDPVDVRYRRFEQDPKPVAQAVMFCLMDVSGSM
                     DEHLKDLAKRFYSLLYLFLSRRYRYVEVVFIRHTHEAKEVDEDTFFNSRETGGTVVST
                     ALEEMARVVADRFPPANWNIYAAQASDGDNLPIDNEKTVALLKSAILPLCQYYAYIEV
                     GREPEERRRGIEPPSTLWRAYDLILSPQLPMAMRKVRDRRDIYPVFRELFERKSNAVS
                     VGD"
BASE COUNT          482 a          725 c          781 g          522 t
ORIGIN      
        1 gggggcgcac agcaggcttt ggagccgtca tagcggtcta ccctttcaga tagttgcctc
       61 ccggtggggg gaggtgtccc gctgcgagcg atatccgcgg ttgtagattg agtcaagcga
      121 aaccggcccg ctataaacct aagtcatagc tgttggccga tctgccaagg atcgtcatcc
      181 cggaagccgc ttgcggctat ccggatcgtt acaagcgctg aggtttgaga cgatcccgga
      241 taacgcctgc ggcgtttccg ggaagacggc ggctcttttg gcgcgaccgg caaactttgc
      301 ttaaacgaaa ggatgaatgg gctcggcgtg atgctttgtt gcgaggtcgc cctgatcatt
      361 cgtctcgacg aggtgcacct cataccccca aagattggcg aggtgctgca gcacgcgctc
      421 ggcgtcatcc tcttccaggg tgaccccatc gacgacgtga tggcgcaact ccaggcgacg
      481 gtcgccttcc agatcgacat cgacgatctg aatgtcgggg tcatgccggg agacgtcgta
      541 atggcgggcc agcgcgcgcc gcacacggtt gtaccctcgc tcattgtgga tggcgccgac
      601 attgagatcc ttttcccgat ggtcgtcgac cacgaggaac aggcccatct tgcggatcac
      661 gtgcgggctc aggaattgcg agacaaaact ctcgtcgcgg tagttcgccc agatatcttt
      721 cagcgtcccc atcgcatcgc cattacccgc catctccggg aaccagtggc ggtcttcctg
      781 cgtcgggtcc acgcaaatgc gctcgatatc cttcatcatg gcgaagccga gcgcgtaggg
      841 gttcagtcca ccgtagcgcc ggtcgtcgaa atcgggttgc atgaccacac ttgtgtggga
      901 atgcaggaac tcaagaaacg agccctcggt gatacgcccc tcatcgagaa ggcgctgcat
      961 gatctggtag tggacgtagg tggcgcagcc ctcgttcatc atcttcgtct ggcgctgcgg
     1021 atagaaatat tgcgcgatca ggcggacgat ccggaggatt tcgcgctgcc agcccttgag
     1081 gcgcggcgag gtcttttcaa ggaaatagag gacgttctct tcgggcaagc cgagaagggc
     1141 gcggcggcgg tcgcgctcgg aacgccgctt cgccttcttg gcctgttcag ggaccgtgcg
     1201 ccacagatcg ttgaacagac gctccccgtg ctcgcggcgc tcgcgctcgc gtttctcttc
     1261 ggagcgcaga tccatctggc gcttgccggg atagcggtgg acaccctggt tctgcaacgc
     1321 gtgggccgcg tcgagcacgc gctcgacttc agcggcgccg tatttgtcct cgcaagcggt
     1381 gatgaaattc ttcgcaaagg agagataatc gaggatcccg tcggcgtcgg tccactgctt
     1441 gaagacatag ttgtttctga agaaatggtt gtggccgaag gctgcatgcg ccagaacaag
     1501 cgtctgcatt gtcgccgtgt tttcttcgag caggtagacg acgcaggggt tcgaattgat
     1561 gacgatctcg taggcgaggc cctgccagcc cttccggtaa agcgtctcgt tgcgcacgaa
     1621 ctgcttgccg aaagaccagt ggcgatagaa gagcggcatg ccggttgagg cgtaagcatc
     1681 gagcatctgc tcggtcgtga tcacctcgat ccggggccgg tagacgttga gccccatttc
     1741 cccgaggccg atttcggcaa cgccgtcata gatgcgcgca agtgtgtcga agtcccagtc
     1801 cgcgcccttg aagagaagcc ggtcctcggc tgcggttgtc gtcatcaatc ccccactgat
     1861 actgcgttac ttttcctttc gaagagctcc cgaaagacag gatagatatc gcgccggtcg
     1921 cggaccttcc gcatcgccat tggaagttgc ggagacagga tcaggtcgta ggcgcgccag
     1981 agcgtgctgg gcggctctat cccgcggcgg cgctcttccg gctctcggcc cacttcgata
     2041 taagcgtaat attggcacaa gggcagaatg gcgctcttga gcagggcaac ggtcttttcg
     2101 ttgtcgatgg gaagattgtc gccgtccgac gcctgggccg cgtaaatgtt ccagtttgcc
     2161 ggcgggaaac gatccgcgac aacgcgcgcc atttcttcaa gcgctgtcga gacgacggtg
     2221 ccgcctgtct cgcggctgtt gaagaacgta tcctcgtcga cctccttcgc ttcgtgcgta
     2281 tggcgaatga agacgacctc cacatagcga tagcggcggc tcaggaacaa atagagcaaa
     2341 ctgtaaaagc gcttcgccag atccttgaga tgctcgtcca tggagcccga cacgtccata
     2401 aggcagaaca tgacggcttg cgcgacgggc ttcgggtcct gttcgaagcg ccggtaacgg
     2461 acgtcgaccg gatcgatgaa cgggatatcg cgggaccgct tggcgaggcg
//
LOCUS       DATMGX010000669         3589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_218314, whole genome shotgun sequence.
ACCESSION   DATMGX010000669 DATMGX010000000
VERSION     DATMGX010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218314"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VNR65_10925"
     CDS             <1..656
                     /locus_tag="VNR65_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWJ19229.1"
                     /translation="DHGQPFKVVGNDSAASGGTPLRKLIRLIPNVLTLAAVVLGLTAI
                     RLSGEGEFALAMTAILGAALLDAADGFVARKLSAESAIGAELDSLADFLNFGVAPAML
                     LYDRHLFSLGMAGWLIAAIYVIATGLRLARFNVQSKAVLPVPADPSRPRKKWFCGLPS
                     TGAAMAIMGADAAVLRLHPAEIPMVIAGAAVTASALMVSKLPVPSLAALLSGSSLKR"
     gene            complement(664..987)
                     /locus_tag="VNR65_10930"
     CDS             complement(664..987)
                     /locus_tag="VNR65_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19230.1"
                     /translation="MSRILLSAALALAVLGTTVETQAAENGYQHRRAHHHSYAPVQRS
                     YEFFSFGSSSEYYAHRPVGGHSYYWQHRAWPFVNGASYNYPGHYNNQTFWERVQTQAN
                     YPIQY"
     gene            complement(1274..1510)
                     /locus_tag="VNR65_10935"
     CDS             complement(1274..1510)
                     /locus_tag="VNR65_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19231.1"
                     /translation="MPAIQMSRGERFSLEKIIKRPCLSTDVPSEHAEKLLNYGLVKRQ
                     VMLLHATPLGQLEILRRRFRRDDFGSSFAMRKAG"
     gene            1689..2063
                     /locus_tag="VNR65_10940"
     CDS             1689..2063
                     /locus_tag="VNR65_10940"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HWJ19232.1"
                     /translation="MTQNGTQEPFASELRLNPQKSELSITFTDGTAGRLSAELLRVIS
                     PSAEVQGHSPSERKLVPGKRDVTIRAIEPVGNYAVRLVFSDGHNTGLYSWLYLHEIAH
                     RKEELWNGYLQELAAAGASRDR"
     gene            2176..3549
                     /locus_tag="VNR65_10945"
     CDS             2176..3549
                     /locus_tag="VNR65_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ19233.1"
                     /translation="MLNSYDKGSDAQSPDILGRPADLAQAIDESEAGTLTTRRAVTRL
                     VEQRRLVLLFALILFSLAIVYGLPWEAQAGALFAAAGGAALIPRSAMKQVIPKVIAAP
                     HPQVRPLHIAASVIDALPEPAVLLSRDGSILASNGKARDLFGGLRPGFHISSATRSPQ
                     VLDAIMECSPEKAQRTVSFSERVPVERYMAATVSHLEGVPENEPVILLFLRDLTEQRR
                     LDQLRSDFIANASHEIKTPLASLVGFIETLQGAARHDEAARDRFLPIMAKQAERMARL
                     IDNLMSLSRVEMRAHLKPRDVVVISELVRHTCDALEPMASQANIDVQVFSELDGATVL
                     GDRDELAQVFINLIHNAIKYGRPGGSVVVKLERQQSGASSVIAISVEDDGPGIGAQHL
                     PRLTERFYRVPGSAAMEKGGTGLGLAIVKHVVNRHRGELRISSTVGAGTKFTVVLAEQ
                     LAAQVTR"
BASE COUNT          770 a         1035 c         1029 g          755 t
ORIGIN      
        1 atgatcatgg ccagcccttc aaggtggtcg gcaacgatag tgccgcttcg gggggaaccc
       61 cgcttcgcaa gctgatacgg ctcattccga acgtgctgac gctcgcggcg gtagtcctcg
      121 gtttgactgc gatacgcctc tcaggcgagg gcgagttcgc tctcgcaatg acggctattc
      181 tcggagcggc tctgcttgac gcggccgatg gttttgtcgc gaggaagctt tcggctgagt
      241 ccgcaatcgg agccgagctc gattctctcg cagatttcct gaatttcggc gtcgctccag
      301 cgatgcttct gtatgaccgc cacctgtttt cgctgggaat ggcaggctgg ctcatcgcgg
      361 cgatatacgt gattgcaacg ggccttcgtc ttgcccggtt caatgttcaa tccaaggccg
      421 ttttgccagt cccggcagac ccttcccggc cgcgaaagaa atggttttgc ggcctgccat
      481 ccacgggcgc cgcgatggcg atcatggggg cggacgcggc ggtgctgcgc ctccaccctg
      541 cggaaattcc gatggtgatt gcgggcgccg cagtcacggc cagcgcgctg atggtgagca
      601 agcttccggt gccgtctttg gcggcacttt taagcggaag ctcactcaag cgttgatgac
      661 aggctaatat tgaatgggat aattcgcctg ggtttgaacc cgctcccaga aggtctgatt
      721 gttatagtgg ccgggataat tgtaggaagc cccattcacg aatggccatg cccggtgttg
      781 ccagtaatac gaatggcctc caaccggacg atgagcgtaa tattcggaag aggagccgaa
      841 cgaaaagaac tcgtaagagc gctggaccgg ggcgtaggag tgatgatggg ctctcctgtg
      901 ttggtagcca ttttcggctg cctgggtctc aaccgttgtg cccaggacag caagggccaa
      961 ggctgcactc aataaaattc tcgacatgaa taatctcccc gctagcgtgc cctttaaagc
     1021 ctgaccggaa ccgaagtttc atcggcccat ccttaccaga cattataggt gcgatggtga
     1081 atcctactag tgaggtttta caacggcgta aatatattgc tgtcctggtc cgattccggc
     1141 aggcacttgc agcgcacaca agagcaaatg ccgcaatcaa aataaccacg agcaccattt
     1201 ttgaatgaac ttttgaattt gacacttgag aacgaacttt tggcaggcga aagccaaatg
     1261 tcaaattcgc tcctcagcct gcttttctca tcgcgaaaga tgagccgaaa tcatctctcc
     1321 tgaaccgtcg ccggagaatt tcgagctgac ccagcggggt cgcgtggagg agcatcacct
     1381 gccgtttgac aaggccgtaa ttcaagagtt tttccgcatg ctccgacgga acgtccgtgg
     1441 aaaggcaggg gcgcttgatg atcttctcaa gcgaaaaacg ctcaccgcga gacatctgga
     1501 ttgctggcat ggatacctac gcaacggtct ggataataca ggcttggaac aaaacgccgc
     1561 ctgattgaga gcctttatgg cggcttcgcc ggcgagcact taagtgtatt aagctggatt
     1621 acggcaaaaa agcgtaatgc ggaaagctga aagccccgta aacggtggac acccagagga
     1681 agaaaagaat gacccaaaat ggcacgcagg aaccattcgc ctccgagttg cggctgaacc
     1741 cgcagaaatc cgagctttcc atcacgttca ccgatgggac cgcgggcagg ctttccgccg
     1801 aactcctgcg cgtgataagc cccagcgcgg aagtccaagg ccactcgcca tcggaacgca
     1861 agctcgtgcc cggcaagcgc gacgtgacca tccgcgccat cgaacctgtc ggaaattatg
     1921 ccgttcgcct cgtgttcagc gacggtcaca acaccggcct ttattcctgg ctctatcttc
     1981 acgagattgc gcaccggaaa gaggagcttt ggaacggcta tctccaagag cttgccgctg
     2041 cgggcgccag ccgggaccgc tgaatccggg ccgccaaatc gagcgttgaa aaaatgtcgc
     2101 aggtttgctg tatgctcgcc tcatgcggca acaattcgtg cgaccacgaa ttctgcacca
     2161 gacaattcat gagacatgct gaacagctac gataagggct cagatgccca atcgccggac
     2221 atcctaggcc gtcccgccga tttggcacag gccattgatg agagtgaagc gggaacgcta
     2281 acaactcgcc gcgccgtcac ccggctggtc gagcagagaa ggcttgttct tctcttcgcc
     2341 ctcatccttt tcagtctagc catcgtgtac gggctgcctt gggaagctca ggcgggcgcg
     2401 cttttcgccg ccgccggcgg tgcggctctc atcccgcgtt ccgccatgaa acaggttatc
     2461 cccaaagtga tcgcagcgcc gcacccgcag gtgagacccc ttcacattgc ggcatcggtc
     2521 atcgatgcac ttccggaacc cgcagttctt ctctcacgcg acggaagcat cctggcatcg
     2581 aacggcaagg cgcgtgatct tttcggcggg ttgagacctg gattccacat ttcgagcgcc
     2641 acccgcagtc ctcaagttct cgacgccatt atggagtgca gccccgaaaa agcgcaacgc
     2701 actgttagct tcagcgaaag agttcctgtc gaacgctaca tggcggcgac ggtttctcac
     2761 ctagaagggg tacccgaaaa cgagccggtt atcctgctct ttctgcggga tttgacagaa
     2821 cagcgccgtc tcgatcagct ccgctccgac ttcatcgcga atgccagtca tgagatcaag
     2881 acgccgcttg catcgctcgt ggggttcatc gagacccttc aaggcgcggc tcgccatgac
     2941 gaagcggcgc gcgaccgttt tctgccgatc atggcgaagc aggccgagcg gatggcacgc
     3001 ctgatcgaca atctcatgtc gctgagccgc gtcgaaatgc gagcgcatct gaagccgcgc
     3061 gacgtcgtcg tgatctctga acttgtccgc cacacctgcg acgcgttgga gcccatggcg
     3121 tcgcaagcca atatcgatgt tcaggttttt tccgagcttg atggcgcaac tgttctggga
     3181 gaccgtgacg agttagcgca ggtctttatc aacctcatac acaacgccat aaagtatggc
     3241 cgcccgggcg gtagcgtcgt ggtcaagctt gagcgtcagc agagcggtgc ctcaagcgtg
     3301 attgccataa gcgtcgagga tgacggcccc ggcattggcg ctcagcatct gccgcgattg
     3361 accgagcgtt tttaccgtgt tccggggtcg gcagccatgg aaaaaggagg caccggcctt
     3421 gggctcgcca tcgtgaagca cgtcgtgaac cgccaccggg gcgaacttcg tatttcgtcg
     3481 acagtgggag ccggcaccaa gtttaccgtc gttctggccg agcagcttgc ggctcaggtt
     3541 acgcggtaag ctgtgacaat tgatacacga gcggagctcg aattgcgcc
//
LOCUS       DATMGX010000670         2013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_226350, whole genome shotgun sequence.
ACCESSION   DATMGX010000670 DATMGX010000000
VERSION     DATMGX010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226350"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VNR65_10950"
     CDS             complement(<1..630)
                     /locus_tag="VNR65_10950"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWJ19234.1"
                     /translation="MIEAHSFAGKTVAVFGMGMSGLATGRSLLAGGASVLAWDDGEKG
                     RDAAKAAGLPLVDLKTIDWGTVDALILAPGVPLTHPDPHWTVKLANAHGVEIIGDTEI
                     FMRERRRAGTASPVIAITGTNGKSTTTALTHHLLEAAGLDAVRGGNIGTAVLDLPPFE
                     DGRYYVLELSSYQIDLTPSLNPDAGILLNITPDHLDRHGTLENYAAVKEK"
     gene            complement(632..1723)
                     /gene="mraY"
                     /locus_tag="VNR65_10955"
     CDS             complement(632..1723)
                     /gene="mraY"
                     /locus_tag="VNR65_10955"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWJ19235.1"
                     /translation="MLYLLGELSKESQIFNLFRYITFRTGGAVVTAMLVVFVLGPWLI
                     DSLRLRQGKGQPIREDGPKSHLLTKKGTPTMGGLMILLGLCVATVLWANPFSPYVWIV
                     LAVTLSFGLIGFYDDYLKVTRRTASGFSGRFRLFLELLVAGLATYAIVAAAGWKPFAT
                     SLTVPFFKDFVIQLGPLLFVCLGIFVIVGAGNAVNLTDGLDGLAIVPVMIAASAFGII
                     AYLVGNKIFADYLQIHFVPGAGELAVMCGALLGAGLGFLWFNAPPAMIFMGDTGSLAL
                     GGALGSIAVAVKHEIVLAIVGGLFVLETVSVIVQVVSFKLTGKRVFRMAPLHHHFEQK
                     GWAEATVVVRFWIIAVILALIGLTTLKLR"
     gene            complement(1758..>2013)
                     /locus_tag="VNR65_10960"
     CDS             complement(1758..>2013)
                     /locus_tag="VNR65_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19236.1"
                     /translation="DLASAVDSNGIDLVFCAGPLMAHLYDRLPLQKKGGWAQKSEDLR
                     AVLLDAVQGGDAVMIKGSLGSRMGHLAEALRLRFSNLATA"
BASE COUNT          451 a          623 c          597 g          342 t
ORIGIN      
        1 cttctctttc accgccgcgt agttctcaag cgttccatgc cggtcgagat gatcgggcgt
       61 gatgttcagc aagatgcctg cgtcaggatt gaggcttgga gtcaggtcga tctgatagct
      121 cgacagttcc agcacgtagt agcgcccatc ctcgaaggga gggaggtcga gaacggcggt
      181 gccgatattg ccgccgcgaa ccgcatcgag gcctgccgcc tcaaggagat ggtgggtcag
      241 cgccgtcgtg gtggattttc cattcgtgcc tgttatggcg atgacaggcg aagcggtacc
      301 agcgcgccgg cgctcgcgca tgaaaatctc ggtgtccccg atgatctcga cgccgtgcgc
      361 attggcaagc ttcacggtcc agtgcgggtc tggatgggtg agcggcacac cgggcgccag
      421 gataagcgcg tcgaccgtac cccaatcgat tgttttcaga tcgacaagag gcaatccggc
      481 cgctttcgcc gcgtcgcgtc ccttctcgcc gtcgtcccag gccagaaccg aagccccgcc
      541 cgcgagcaaa gaacgccccg tcgccagccc ggacattccc attccgaaaa cggcgacggt
      601 cttgcccgca aaggagtgcg cttcgatcat gttacctcag cttcagcgtg gtcaatccga
      661 taagggcaag aatcacggca atgatccaga aacgcacgac aacggttgcc tcggcccagc
      721 ccttctgctc gaaatggtga tgaagcggtg ccattcgaaa gacgcgtttc cctgtcagct
      781 tgaaggacac cacctgcaca atcaccgaaa cggtctcaag cacgaacaag ccgcccacaa
      841 tggcgagaac gatctcatgc ttgacggcaa cggcgatgga gccgagcgcg ccgcccagag
      901 ccagcgagcc ggtgtcgccc atgaagatca tggcaggcgg cgcgttgaac cataagaatc
      961 cgagccccgc cccaagaagc gccccgcaca taacggccaa ttcgcctgcg ccgggtacaa
     1021 agtggatttg aaggtagtcg gcgaagattt tgttccctac gaggtacgcg atgatcccaa
     1081 aggccgacgc cgcaatcatg accggcacaa tggcgagccc atcgagcccg tcggtcaaat
     1141 tcaccgcgtt gccggcgccc acgatgacaa agatcccgag gcaaacgaaa agcagcggtc
     1201 cgagctggat cacaaaatct ttgaaaaacg gcacggtcag cgacgttgcg aacggcttcc
     1261 agccagcggc ggcgactatg gcgtaggtgg cgaggccagc aacgagcagt tccaggaaaa
     1321 gccggaaccg cccagagaac cccgatgcgg tgcgcctcgt gaccttcaga tagtcgtcgt
     1381 agaagccgat gagcccgaac gagagcgtaa cagccagaac gatccacaca taggggctaa
     1441 agggattggc ccaaagcacc gtcgccacgc acagcccgag caggatcatg aggccgccca
     1501 tggtgggcgt gcccttcttg gtgaggaggt gtgattttgg tccgtcctcg cggatcggct
     1561 ggcctttgcc ttggcggagg cgcagcgaat cgatcagcca ggggccgagc acgaacacga
     1621 cgagcatcgc cgtcacgacc gctccgcccg tccggaacgt gatgtaccgg aacaggttga
     1681 agatttgcga ttccttgctc aattcgccga gtagataaag catccgatgt ccaatttaag
     1741 ccgcaccgcg gcgaacgtta tgcggtagcc agattcgaga agcgcaaacg cagcgcctcc
     1801 gccaagtggc ccatccggct tccgagtgac cccttgatca tgaccgcgtc gccaccctgc
     1861 accgcatcca gcagaacagc gcgcagatcc tcggattttt gagcccagcc gcccttcttt
     1921 tgcagcggca gccggtcata aagatgggcc atcaatggtc cggcgcagaa aacgagatcg
     1981 atgccgttcg agtcaaccgc gctcgcaaga tcg
//
LOCUS       DATMGX010000671         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_323390, whole genome shotgun sequence.
ACCESSION   DATMGX010000671 DATMGX010000000
VERSION     DATMGX010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323390"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VNR65_10965"
     CDS             <1..291
                     /locus_tag="VNR65_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ19237.1"
                     /translation="DRRSDRAPLRKQPPRDLGHRGLASVMRGLVTEIVRRSDRAKGFV
                     VVHKRWIVERTIGWLGRCRRLAKDWENLNRNALAFLRLASIRLMLRKLCNPS"
     gene            413..1477
                     /locus_tag="VNR65_10970"
     CDS             413..1477
                     /locus_tag="VNR65_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ19238.1"
                     /translation="MKYFAGMDVSLEETAICVVDETGQMIKEARATSEPDALHALLAS
                     FNLPFERVGLEACSLTAWLHGELTSAGWPAICIESRHAKAAMGAMPNKTDRNDARAIA
                     QIMRTGWFKAVHVKTPTCRSWRSLLVARRAVLNQMRTIENVVRAILRETGLKLGTPAR
                     KDFIVRAREFVAGEKGLAMMIDPLLEILATMLREFTRLTKQVLVIVRDEPVCRRLMST
                     PGVGPMTALAFRATIDRPERFKKSRDVGAHLGLTPRRYQSGETDIQGRISRCGDELAR
                     TALYEAAHSLLIRSKKWSALRAWGMKIAKRRGMARARVAVARKLATILHRMWVDNTEF
                     RFGREQAANPTIKAITVVAA"
BASE COUNT          466 a          612 c          645 g          399 t
ORIGIN      
        1 gatcggcgtt cagaccgtgc gccgcttcgc aaacagccgc cgagggatct cgggcatcgc
       61 gggttggcga gcgtcatgcg gggcctcgtc accgaaatcg tccgccgttc cgatcgcgcc
      121 aaaggctttg tcgtcgtcca caaacgatgg attgtcgaaa gaaccatagg ttggctgggt
      181 cgctgccgcc gcttggcaaa ggactgggaa aacctcaacc gaaacgccct cgccttcctc
      241 cgcctcgcct ccatccgcct gatgctcaga aaactctgca atccctcatg aagtcttcgg
      301 acagatgggg tgattggggt ctcggcccgg agacgacgaa ggcggctgct cccgtcagga
      361 tgtgagaacg tggtcggtgt cgaatcgcca caaacatcga gaaggagcag ccatgaagta
      421 ctttgccgga atggacgtgt ctttggaaga gaccgcgatt tgcgttgttg atgagaccgg
      481 tcaaatgatc aaggaggctc gcgcaaccag cgaaccggat gcgctccatg ctctccttgc
      541 cagcttcaat ttgcccttcg aacgcgtggg actggaagcc tgttcgctga cggcttggtt
      601 gcatggcgag ctgacttccg ctgggtggcc ggcgatctgc atcgagtcgc gacacgcgaa
      661 ggccgcgatg ggcgcgatgc cgaacaagac cgaccgcaat gacgcgcgcg ccatcgccca
      721 gatcatgcga accggctggt tcaaggcggt tcatgtgaag acgccgacat gtcgctcctg
      781 gcgttcgctg ctggttgcgc gccgcgcggt cctcaaccag atgcggacga tcgagaatgt
      841 cgtgagagcc atccttcgag agaccggcct gaaacttgga actcccgcgc gcaaggactt
      901 cattgttcgc gcccgcgaat ttgtcgctgg agagaagggc ctggcgatga tgatcgatcc
      961 gctgctcgaa atcctcgcca cgatgctgcg cgagttcacc aggctgacca agcaggtgct
     1021 cgtcatcgtc cgggacgaac cggtctgccg acgactgatg agcacgccgg gcgtcggtcc
     1081 gatgacggcg ctggctttcc gggcgacgat cgaccggcca gaacgattca agaagtcgag
     1141 ggatgtggga gctcatctgg gcttgacgcc acgacgctac caatccggag aaaccgatat
     1201 tcaagggcgg atcagccgat gtggcgatga gctggcgcgc acggcgctct acgaagcagc
     1261 ccattcactt ctcattcgca gcaaaaaatg gtcggccttg cgagcgtggg gcatgaagat
     1321 cgccaaacgt cgcggaatgg cgcgagcccg cgtcgcggtc gcgcgaaagc tcgcaaccat
     1381 tctgcaccgc atgtgggtcg ataacaccga attccgtttt ggccgcgaac aggcggctaa
     1441 cccaaccatc aaggcgatca cggtggtcgc ggcctgagga ggtaaaccta aagccatcga
     1501 atagtccgcg ccagcggaag gaatcgtccc cgtggggacg atgggcaagg cgatcccgtt
     1561 gagagtccgg agccgaaggg cgcgagcccc gaaggtcgtg aaacagattg ggacgcctcg
     1621 ctcttctgac cccaccatgc ggcggccaat gcgccgaccg cgaagagaag cgagtagccc
     1681 acgggaacga gatcaaccgc cagcaaggaa aaaagcagga gcagcttgac ctcagggacc
     1741 ccaatcagag aggactctaa gcccgtcaat ctgcaatcgg tgtcaaattg aattctaaga
     1801 atttggcctt gtttggccaa tatattttga ttcgagatga cgttaatgtc gatatagcaa
     1861 aataattcca gacgtcgatt cagtgctttg cgaacgaaat acacaattat aacacgatta
     1921 ccctcagttt tgccacgttg ttattatgga gtggaagggt ccggactcca gcttcatctg
     1981 gaggaagttg cggggcgcag caagagaaca aagcgcgcaa cccggcgttg acctcgtgtg
     2041 ctaaggcccc ccgcgccgtt ctgcgctccc gtccagaggg ctttctcgct tctgttgtgc
     2101 agttccaacc ccttgaagac ga
//
LOCUS       DATMGX010000672         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_296379, whole genome shotgun sequence.
ACCESSION   DATMGX010000672 DATMGX010000000
VERSION     DATMGX010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296379"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VNR65_10975"
     CDS             complement(<1..436)
                     /locus_tag="VNR65_10975"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWJ19239.1"
                     /translation="MLGLVEHKAMPVYPDEAMKKGIHGDVIFKIVVDDTGTIVWSTPV
                     EGDPLLVAASVDALRDFRFRPYLLNGSPVAFVESQLGFHFSVGKNAGEASGHVECMSS
                     IPYRTEFRTGVVSDKGVLVLAPRKVSGVEPQLPPELAGKSGTV"
     gene            complement(708..>2132)
                     /gene="lepA"
                     /locus_tag="VNR65_10980"
     CDS             complement(708..>2132)
                     /gene="lepA"
                     /locus_tag="VNR65_10980"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWJ19240.1"
                     /translation="IPVINKIDLQSADIESTKEMIESAVGLDASDALLISAKTGQGVP
                     DVLEAIVKRVPPPKGAPERHLQALLFDSWFDPYRGVVVLARVFEGRLRKGQKIRLWSN
                     GHVFQVETLGVLTPKPVEIDELVAGEVGFIIANIKNVADTKIGDTITDDDRPAIQALP
                     GFEEIKPMVFAGLYTVDAHEHTQLREALEKLKLNDSSFFFEPESSVALGFRFRCGFLG
                     LLHMEIIQERLEREYNLDLITTAPGVRYLITRTDGTVIEVDNPSRWPDVSEIAKVEEP
                     VILAVILTNEEYVGGILKLVEEKRGKQKSFEYVSSSRVMLQYELPLNEIVLDFYDRLK
                     SVSRGYASLDYHLSGNWTSPMSKLDIMVAGEPVDALSTIIHRDFAYDRGRMLAAKMKE
                     LIPRQMFEVAIQAAIGAKIIARETVPAIRKNVIAKCYGGDISRKRKLLEKQKAGKKRM
                     KRIGRVDIPQEAFLAVLKVGEGSS"
BASE COUNT          448 a          675 c          551 g          458 t
ORIGIN      
        1 ataccgtccc cgacttaccc gctaattccg gcggcaactg cggctcaacg cccgacacct
       61 tgcgaggcgc gagcaccagg acacccttgt cgctcacgac gcctgtccga aactccgtcc
      121 gatagggaat cgacgacatg cactcgacat gcccactagc ctcgccggca tttttcccca
      181 ctgagaaatg aaaacccaat tggctctcga cgaatgcgac cggagatcca ttgagcaggt
      241 agggccggaa gcggaaatct cgaagcgcat caacactcgc agctacaagc agagggtcgc
      301 cctcgacggg cgtactccat acgatcgttc ccgtgtcatc gacgacgatc ttgaaaatta
      361 cgtcaccatg aattcccttc ttcatggcct cgtcggggta caccggcatc gctttgtgtt
      421 cgaccagtcc cagcatcact ccgccagaaa cgcgaaccct caagggccga gggggttgaa
      481 cttgggattg agaatcagcg ctattttggg cacgcccaat cgttgtgcag catgcgagca
      541 acgcagcgat catcttgatt cgtaacatgc aaccctccta ccagtggccc gccatcccat
      601 tagtcccaac tctgagcgtg cctcgtccaa gctcggcttg ggcgggtctt ttctcggacc
      661 tggctatgcg gcaagtcgag agtcttgacg gcccttcgaa tggaatccta gctgctgcct
      721 tcgccgactt tcaggacggc gaggaatgct tcctgcggaa tgtccacccg cccgatgcgc
      781 ttcatccgtt tcttgcctgc cttttgtttc tccagcagct tgcgtttgcg ggaaatatcg
      841 ccgccgtagc acttcgcgat cacgttcttg cggatcgcgg gaacggtttc gcgggcgatg
      901 atcttcgccc cgatcgccgc ctggattgcc acctcaaaca tctggcgcgg gatcagttcc
      961 ttcattttcg ccgccagcat ccgcccgcga tcataagcaa agtcgcgatg gatgatggtc
     1021 gacaacgcat cgaccggctc gcccgctacc ataatgtcga gcttcgacat cggagaagtc
     1081 cagttgcccg acagatgata gtcgagcgaa gcgtatcccc gggacacgga cttcaggcgg
     1141 tcataaaaat cgagcacgat ttcattcagc ggcaactcat actgcagcat aacccgcgac
     1201 gagctgacgt attcaaaact tttctgcttc ccgcgtttct cctcgaccag cttgagaatg
     1261 ccgccgacat attcttcgtt ggtcaaaatt accgcgagaa tcaccggctc ttccacctta
     1321 gcgatctcgc tcacatccgg ccagcgcgaa ggattatcca cttcgatcac cgtgccgtcc
     1381 gtcctggtga tcaggtagcg cacgccggga gcggtcgtga tcaggtcgag attgtactcg
     1441 cgctcgaggc gttcctggat gatctccatg tgcaagaggc cgagaaagcc gcagcggaag
     1501 cggaagccga gcgccaccga actttccggc tcaaagaaga atgatgaatc gttcagcttc
     1561 aatttttcca gcgcctcgcg caactgtgta tgttcatgcg cgtccacggt atacagtccc
     1621 gcgaaaacca tgggcttgat ttcttcaaag cccggcaatg cctggatcgc gggacgatca
     1681 tcgtccgtaa tggtgtcgcc gatcttcgta tccgcgacgt tcttgatatt cgcgatgatg
     1741 aatccaacct cgcccgccac cagttcgtcg atctccaccg gcttcggcgt gaggacgccc
     1801 agagtttcga cttgaaaaac gtgcccgttt gaccacagcc ggatcttttg ccccttgcgc
     1861 aaccgaccct cgaaaacgcg ggccagcacc actaccccgc gatagggatc gaaccaggaa
     1921 tcaaacagca acgcctgaag atgccgctcc ggcgcgcctt tcggaggcgg aacgcgtttc
     1981 acaatcgcct cgagaacatc cggaacgccc tgcccggtct tcgcgctgat cagcagagcg
     2041 tcggatgcgt cgagaccgac tgcgctctcg atcatctcct tggtgctctc aatgtcggcg
     2101 ctctgcaagt cgatcttgtt gatcactgga at
//
LOCUS       DATMGX010000673        20411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_195298, whole genome shotgun sequence.
ACCESSION   DATMGX010000673 DATMGX010000000
VERSION     DATMGX010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 20411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20411
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195298"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VNR65_10985"
     CDS             complement(<1..1427)
                     /locus_tag="VNR65_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWJ19241.1"
                     /translation="MTGLAQYGADLALPGMLVGRILRSPHAHARIRSIDTAKAAVLPG
                     VKAVVTSADFPEQKFEYVGPERVAQNFWHMTRNIMAREKVLYEGHAVAAVAAISKAVA
                     DEALSLIAVDYEVLPHVIDVDEAMKPDAPLLFPDLITRGVDPPPKPSNISKRAEFNIG
                     DLEAGFAAADEIVEMSFKTAPVHQAYIEPQGCVARFDADGQSELWSSSQGHFVVRAYT
                     ARLLGMKLGDLRVYPAEIGGAFGGKTVIYVEPVAVILARKSGHPVKIVMSREDVFRAT
                     GPASGSSMTVKIGVKRDGTIVAADGVFKLQAGAFPGSPMMSTCMCAFAPYDIANARSV
                     GYDVVSNRPKVAAYRAPGSPIGAFAVESVLDVVAQKIGMDPLKLRLKNAAKKGTQMLS
                     GTKLSHNGYAETIEAVLNHPGYKAPLRKNQGRGVASGYWFNGGGESSATMHVNEDGTI
                     VVATGSPDIGGSRASMAIMAAETLG"
     gene            1704..2570
                     /locus_tag="VNR65_10990"
     CDS             1704..2570
                     /locus_tag="VNR65_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWJ19242.1"
                     /translation="MLSYTAPSTVEEAVRILAGASGTAKVLSGGTDLLVQLRSGRAKP
                     DLIVDIKNISGISDIREQDGVFIIGAATPGAVIGECEALRQAWPGVVEAANLIGSTQV
                     QGRASLAGNLCNASPAADSVPALIAARATCVVAGAKGRREVPVERIPTGPGRTCLRPD
                     EFIVEFHLPKRLPHSADAYLRFIPRTEMDIAVVGVGVSVTLDTRGVCTEASVVLGAVA
                     PTAILVPEAAAALVGHNCDETALSELEQAARRACKPINDKRGTIEYRTKVAGVMARRA
                     AVIAYERAGERR"
     gene            2567..3043
                     /locus_tag="VNR65_10995"
     CDS             2567..3043
                     /locus_tag="VNR65_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWJ19243.1"
                     /translation="MSKYHISTTINGEPAEFLCEAQQTLLDVLRDEVHLTGSKEGCSS
                     GDCGACSVTVDGRLVCSCLMLAVEAEGHSIETIEGMAQGQDLHPLQRKFLEHAALQCG
                     FCTPGVLVAAKALLDRNPNPTETETRYWLAGNLCRCTGYDKIIRAVLDAAAELRGV"
     gene            3648..4961
                     /locus_tag="VNR65_11000"
     CDS             3648..4961
                     /locus_tag="VNR65_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HWJ19244.1"
                     /translation="MKGGGGFGWLGDCYLGGHGFRRAMIAATASGFFNSLLSGKRTGP
                     GIVGTGQPIPWPATMGCQAGTAVVRAASPSCQCAEKKAKIHTKSGVIWGIPDKIGPWE
                     CDFDLGGEPMKILRRKWLHLAALSVVLAGVAAINEPAWPQTGRPIRIIIPFPPGGAAD
                     ILARILSQQIGDANNQTMIVENHPGAGASIAYELTARATPDGNTIVVAANSLVINPIL
                     RKTNYDPVTSYEPICNLVTSPMVFVVNSVSSYKTIGDLIAAAQAKPGTLTLAALGPAT
                     TQHIAVEQFKLVTKVDMIFVPYPGGAPVINALLGDQVTAGLVNYSEAVQQIKAGKLRA
                     IATASNKRIEPLPDVPTVAESGYPSYESEVWLGLFAPAKTPKKSIAQLIAWFTAAMKT
                     PVVEAKLLPLGLYSAVVCGKDFGAFVKQRSDEYARIIHDANIKME"
     gene            complement(5510..7465)
                     /locus_tag="VNR65_11005"
     CDS             complement(5510..7465)
                     /locus_tag="VNR65_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HWJ19245.1"
                     /translation="MTWSNDRSHTLGHYDATNAADAGIGPQGTALICELRRLQKFLNK
                     ESYQEREQFTHAVEARPANWLFDNRRGTRIEPTRTAVDGAVNFVLKMIGLNRRKSIGY
                     GSKSRQRGKTPALPRRSGPQRVKVFSQPRAGQPTEGATPPLTTAKIESRDTHQASAYS
                     SIKRPSPIPSPAETTNSLRRGFQSGLEFLRSGSAFEHLRSACAFLISRAGATRLDETS
                     GAGLTARLTHAFEGELKIGLRVLILGVGILGGWATLVPLSGAVVVPGTLVVESDVKKI
                     QHPAGGVVAYIPVRDGMHVRAGDLLLRLDDTQLQANYQVLMQQLDQVRVRLARLAAER
                     DGVDQPRKPQEMSTRLGEDNVSHLWASEISLFSSRAAARRSAKELLRSHVGQLGEQIS
                     GLDAQVKSKAAQHDLISRELEGVDSLYQKGLVPLTRKTSLEREAARLDGDRGQTTAAI
                     AEAKSKISEAELQAVRIDQDFRSEVMKDLREAQDKEAELVQKTVAAQDLLKRVDLRAP
                     TNGVIHQLSVHTVGGVIAPGEVAMEIVPESDELQIEAKLPPRDIDQVGSGQRAYVRFS
                     AFNQRTTPQLEGVVSYVSADLSHDRQANAAYYTVRVTLPSSERRRLGSLQLVAGMPVE
                     VFLQTGSRTMTSYLLKPITDQLLRTFN"
     gene            complement(7462..9216)
                     /locus_tag="VNR65_11010"
     CDS             complement(7462..9216)
                     /locus_tag="VNR65_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HWJ19246.1"
                     /translation="MLSLEGDDPVAIQLRASGRRLAGVAVFSGVINLLTLSGSLYMLQ
                     VYDRVIPSRNLATLFGLSAIVMVAYLLQGYFDALRGRMLARIAALFDVQLQTRIHLAL
                     VTLALRGTKPILAQQPLRDLDQVRAFLSGAGPTAFLDMPWIPLFLLALFLFHPVIGVI
                     ATGGAAAIIAMTFLTERQSKSAAKMSMEGNAQRQVLADALRQNAEVIRALGMTGRLAD
                     RWSLTNEHYIHHNTRLADVHANLSAAAKVLRFVLQSAVLGVGAYLVVIEQASGGIMIA
                     SSIIMGRALAPVEVALANWKQLVAAREGINRLRTVLKVTAAPAAPAVVLQRPNRILTT
                     ENLSVIVPGTQKAVLSQVSFGLRAGMGLALLGASAAGKSSLAKALVGIWPAATGAVRL
                     DGAAIDRWHPDDLGRHIGYLPQDVALFDGTVAMNIARFDEEATSEAILEAAQVAGVHE
                     MILRLPDGYDTRIGERGASLSAGQRQRVGLARAVYGRPFLVVLDEPNANLDAEGENAL
                     VKAIETLRSEQSIVIVVSHRSNALAVLNMTMVIHEGRSIAFGPSEEIFARVARTVDRS
                     AEAPKQSGASQAVAGAAR"
     gene            9578..10888
                     /locus_tag="VNR65_11015"
     CDS             9578..10888
                     /locus_tag="VNR65_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19247.1"
                     /translation="MAHDTQGHHGDTNTPTPPEKPHLPMTFAEIGTLYNSISDELEGG
                     VTHQNQQLLLNQVSTVQTQLQSLINSGALNNLDGAGNPAVSVVHAQNVADQMNFLKTE
                     IGAFGTGTFVPKFINDVVRDVQDIVAADPALTALAHQANHSGFQQVSYLLTNPTPFAD
                     SGAQTDALLKFITDSNSLAARAQVLAGTDPNSAPVHQLESDIQAFSTAADSYSTEQGG
                     LFSARFNNEFTLNGVQGTASREMITGLETGNADLVNGAASVLTANANDVRGNMLTQGN
                     TFTPAPNGGIPDFIDTVHDAGVVFNDAMTKLIGGVYSGNQQSILNDLNATAVGLHNAI
                     MDQGITGQALHDINHVISLLGQQASLVGGIGAASPTQTSAVNGQINQIQAQILNVVNH
                     DATLAAMAVGADGTVGFVALPPGTTPSHSDAVASAGHHFEHMWG"
     gene            11115..11855
                     /locus_tag="VNR65_11020"
     CDS             11115..11855
                     /locus_tag="VNR65_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWJ19248.1"
                     /translation="MSRCASRNRRTDYGTVILHWCLVVALTVAVLSGLRIASESPDRS
                     WINALDLILPRRLVWTTHMQAAVALIPIAIAYPIYVSLARLWGRVRFDRIRLSGLFGR
                     HQARWGAVNVALYWIFYLMLLSEIATGILVYFDYGNSTIITLHWLGMSFICGCTIAHV
                     LVHWKIGKASQLLRIVRPARLLPQPPPFDPIDLLELLDQRASEPQESQVSRAMHLRPE
                     LYNDALQQFVPANDHFPLGRREPKQNLF"
     gene            11909..13048
                     /locus_tag="VNR65_11025"
     CDS             11909..13048
                     /locus_tag="VNR65_11025"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HWJ19249.1"
                     /translation="MLQSNPFIVALVVAVAGTSLIVTAERQTSESLLIRRVGAPDIPV
                     IDGDTSDRAWQMTKPLYVLTAHGDNFNGKGETTVEIRAVHDGQWAYFLFVWDDPTRSL
                     KQLPLLKTAEGWKLLHDGYEAGAEHAYSEDKFSVLLTKLNVILAGDRTFHAGTQPLDG
                     KPRTLTGRGLHYTERPGLFADVWEWKATSTSPAGFMDDDHFGPPVEATSAQAEGKIPY
                     RGGFAADPGTASYVDNFAVEDPNDYRKFVTPLRLPKDVSTMTTALGVIDLDPNHGEGV
                     DARWFMTMEESTPYSRDLDAKIPVGTIVPGVIFSGTYSGDRADVRCAARWAAGRWALE
                     VARRLDTGSPYDVPISTGTFMRVAAFDHTQIGHTRHVRPIRLEVE"
     gene            13048..14061
                     /locus_tag="VNR65_11030"
     CDS             13048..14061
                     /locus_tag="VNR65_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWJ19250.1"
                     /translation="MGKVCSLEVNGNSISVHRGDLLLDAALLNGIDIPHDCRSGNCGT
                     CRVRLLDGRVFGGQTDDPEMVYACQSRIVTDLRVHVEDVPEIVTMPGVVADLIPLAPD
                     VCEVCIETRRPVEYFPGQYYAVQFRGFPARYFSPTSALDWPCNEALVRFHIRIVPNGR
                     ISSALGQRIGSGHRVKLEGPFGSAYLRAFPDQRLVLVASGSGFAPIWSIAETAIKSAP
                     RRELVLIVTARSLDSLYMIPALCRLALFPNVVIIPVVSENQDVTAAVRRGKPTDHLPN
                     LTPRDIVFAAGAPEMVRAVARMSGAAGAKCYTDPFEAASHRTATDLWSLAVSWLTNTS
                     GRR"
     gene            complement(14509..16011)
                     /locus_tag="VNR65_11035"
     CDS             complement(14509..16011)
                     /locus_tag="VNR65_11035"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWJ19251.1"
                     /translation="MLTRRTDRPPPTPKHAKEILERIRQDGYDGSEKTLKNHLGDLSR
                     RRYQFWGELYEVLSALDRDRAVRFLFYLYRADTENQTRAASLLQACKLITNVEWRMDP
                     RDSARRLDSEWKCRLAFGLLKIEEVREDVGDIPDLKQLMATLRNGKHYNRMKAIAFLA
                     HRKGIKKTTICSFAGMNKSFVDKNLKICDRDGVKAMLAPPAGHPRRREEEGLRRSVFR
                     ILHAPPRDHGFNRAAWSMQTLREALINDGNPACADVIRQILKSAGYSWRKARIVLTSN
                     DTFYKEKVARVREVASQLRNDEALFSIDEFGPFHVRKMGGRSIVAPGKTPVVQQRQKS
                     RGLIILTAAIELSSNQVTHFYSDRKNSAEMIKMLRLLRKKYRRKRKLWISWDAASWHK
                     SDAIDTFIDHNNGGKWPKIESLLLPASGQYLNIIESIFSGMARAIIHNSDYVSLEECR
                     KAIDEYFLERNTNFRLKPQRAGKNIWGKEREPVAFSEGNDCKDPAYHWAR"
     gene            complement(16301..16570)
                     /locus_tag="VNR65_11040"
     CDS             complement(16301..16570)
                     /locus_tag="VNR65_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19252.1"
                     /translation="MLKTLPIKLVVLATLIIGSTFPAIASLTLFSSADQAQQHCPNDV
                     VVWLNLPTHIYHWKGMRWYGHTKNGAYVCKKEADDNGNRGTLNGQ"
     gene            complement(17052..17861)
                     /locus_tag="VNR65_11045"
     CDS             complement(17052..17861)
                     /locus_tag="VNR65_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ19253.1"
                     /translation="MIVQVNGSSFNCQTDGDRGPWVMLSHGLATDMSMWDELVEALSD
                     RYRVLRYDARGHGGSAAPAGDYTLDMLVADAVGILDALGVEQCHVAGLSMGGMIAIGM
                     LINHPKRVKSAVIADSRHTTTPEFTEAWLQRADAVRKQGIEAIVESTVSRWSSAGLAE
                     RNPAAIAQMDSMIRKTSANGYCGCAAALARLDYGSRLKEINAPALVICGSEDHGAPPA
                     NSRQMQAMIKDARFLEINQAGHISNIEQPIIFNAAVRNFLDDVESRQALSN"
     gene            18121..19134
                     /locus_tag="VNR65_11050"
     CDS             18121..19134
                     /locus_tag="VNR65_11050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HWJ19254.1"
                     /translation="MKREKAALRSVAEFRIVSLAIAIVAVMVGLASPARAQADYPNRP
                     VQLLIAYGAGTIGDVSMRILAQKLSSELGKSFVVENRPGAAGVIAAKAAAAAAPDGYT
                     LLLTGNSYAISTALFKSLPYDVLKDFAPISTVATFDFLVATRKDSKFKSMKDVIAYAM
                     ANPGKLNIATLSPGTTQYLAVELIKVAAGVNVTAVPFRSSPDAVAALLRGDVDIDMDS
                     YAPLKSLLEADKIGVIATTGRKRVGFLTGVPTVMESGLPNFDVSSWNGIAAPAGVPAP
                     IVAKLSKAVNEALQSSEAQATGRRLGMEMRGSTPQELEARLKGDIAKWSDLIEKAHIP
                     KHD"
     gene            19391..19921
                     /locus_tag="VNR65_11055"
     CDS             19391..19921
                     /locus_tag="VNR65_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWJ19255.1"
                     /translation="MKILAAIGSLAIVLAVVAAVYFFGGFYSVAGSQEDPAILHWALV
                     KVRTNSIVRHANDQPPAGFDDPAKVQAGAREFAEHGCVNCHGAPGVKWAKFSEGLHPD
                     PPDLKEVVAEATPSQLFWVIKNGINMTGMPGFGAAGATDEDIWAIVAFLQKLPNVSEA
                     DYKTWTAPAPAPPSKP"
     gene            complement(19936..>20411)
                     /locus_tag="VNR65_11060"
     CDS             complement(19936..>20411)
                     /locus_tag="VNR65_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19256.1"
                     /translation="VQTMDDESRRKSAFPFLAQLADTIGMHANPRMISGAILLTMLVY
                     AVETTKFAGAWIDYKAAVRALATGTDSDPALGDPQFVSSQRIGAGLNRLAWNSTTPYL
                     SVLVAPGLKPTRLVVDPDAGYFWLSCKTATQSELNSTAIPAAARRLIRVHACLHR"
BASE COUNT         4273 a         5987 c         5734 g         4417 t
ORIGIN      
        1 atccccagcg tttcagccgc catgatcgcc atcgaggcgc gcgacccgcc gatgtctgga
       61 ctaccggtcg cgacgacgat ggtgccatcc tcgttgacat gcatggtggc gctcgattcg
      121 ccgccgccgt tgaaccaata gcctgaggcg acgccgcgcc cctggttctt gcggagcggc
      181 gccttgtagc ccggatggtt gagcaccgcc tcgatggtct cggcgtatcc attgtgtgaa
      241 agtttggtgc cagacagcat ctgggtgccc ttcttggcgg cattcttgag ccgcagtttc
      301 agcgggtcca tgccgatctt ctgagccacg acatcaagca cgctctccac cgcgaaggcg
      361 ccgatcggcg atcccggcgc gcgataggcc gcgaccttcg ggcggttgga cacaacgtcg
      421 taaccgaccg agcgcgcgtt cgcgatgtcg taaggcgcaa aggcgcacat gcacgtgctc
      481 atcatcggag agcccggaaa tgcgccggcc tgcaatttga agaccccgtc ggcggcgaca
      541 atcgtgccgt cgcgcttgac accgatcttc acggtcatag acgagccgga cgccgggccg
      601 gtggccctga acacgtcctc tcgactcatc acgatcttca ccggatggcc ggattttcgc
      661 gcgagtatga cagcgaccgg ctcgacgtag atcaccgtct tgccgccgaa agcgccgccg
      721 atctccgccg ggtagacgcg caggtcgccg agcttcatgc cgagcagtcg tgcggtataa
      781 gcgcggacga cgaaatgtcc ctggctcgaa ctccaaagct cgctctggcc gtcggcgtcg
      841 aatcgcgcca cgcaaccttg cggctcgata taggcctgat gcaccggcgc agtcttgaac
      901 gacatttcga cgatctcatc ggccgccgcg aacccggctt ccaaatcgcc gatgttgaat
      961 tcggcccgct tcgaaatatt ggagggtttt ggcggcggat cgaccccgcg ggtgattaaa
     1021 tcgggaaaca gaagcggcgc atcgggcttc atcgcctcat cgacgtcgat gacgtgcggc
     1081 agaacctcgt agtcgaccgc gatcagcgac agcgcttcgt cggcgacggc cttgctgatg
     1141 gccgccaccg cggccaccgc atgaccctca tagagcacct tctcacgcgc catgatgttg
     1201 cgcgtcatgt gccagaagtt ctgcgcgacg cgttctgggc cgacgtattc gaacttctgc
     1261 tctgggaagt ccgccgaggt gactacagcc ttgacaccag gcaggaccgc cgctttcgcg
     1321 gtatcgatcg atcgaatacg cgcatgggcg tgcggtgagc gcaggatgcg gcccaccagc
     1381 atgccaggca acgccaagtc tgcgccgtac tgcgcgaggc ccgtcacctt cggaacgccg
     1441 tccgggcgaa ccggccgcgt tccaacccac ttgaagggtt tgattgtgtc gcgtgaagtg
     1501 atgtcgtcca cagctccctc gcctgattgc cgcgttgcgc ttggctctgc aggagagttg
     1561 cgcggggagg gctcaaatgc aagtcgtcct ccaatcgggt tggcagtcca ccccccgccg
     1621 tcgcgaagcc tcgcttcggc ctagaccgct aaccagcact ggactagcgt tccggttggc
     1681 tcgtttagga gacccctaat ccaatgctta gctacaccgc cccgtcgacg gtcgaggagg
     1741 ccgttcgcat tttggctggc gcgtccggca cagcgaaggt cctatccggc ggcaccgatc
     1801 tgctggttca actgcgttcc ggccgcgcca agcctgatct tatcgtcgat atcaagaaca
     1861 tctccggaat atccgacatc cgcgagcaag atggtgtctt tataatcggt gctgcgacgc
     1921 ccggcgccgt gatcggcgaa tgcgaagccc tccggcaagc ttggccaggc gtggttgagg
     1981 cggccaattt gatcggctcg acccaggtgc agggccgggc gtcgcttgcc ggcaatctct
     2041 gcaatgcatc gccggcggcc gacagcgttc cagcgctgat cgcagcccgc gcaacctgcg
     2101 tggtcgcagg cgcaaaaggg cggcgcgagg ttccggtgga gaggattccc accggtccgg
     2161 gacggacttg cttgcgcccg gatgagttca tcgtcgaatt tcacctgccg aaacggctac
     2221 cacactcggc ggatgcttac ctgcgcttta ttccgcggac ggagatggat attgccgtgg
     2281 tcggtgtggg agttagcgtt acgcttgaca ctaggggcgt ctgcaccgaa gcgagtgtcg
     2341 tgctcggtgc tgtggcgccg accgcaatcc tggttcccga agccgccgcc gctttggtcg
     2401 ggcacaactg cgacgagaca gccctctccg agcttgagca ggcggcgcga cgcgcctgca
     2461 aaccgatcaa cgacaagcgc gggacgatcg aataccggac taaagtcgcc ggcgtgatgg
     2521 cgcgtcgggc ggcggttatc gcctacgagc gtgcgggaga gcgtcgatga gcaagtatca
     2581 catatcgact acgatcaacg gcgagcccgc ggaatttctg tgtgaagcgc agcagactct
     2641 gttggatgtg ttgcgtgacg aagttcattt aaccggcagc aaggagggat gcagttcggg
     2701 cgattgcggc gcctgcagcg tgacggtcga cggtcgactg gtctgctcgt gcctcatgct
     2761 tgcggtcgaa gccgaagggc attcgattga gacgatcgag ggcatggcgc aaggtcaaga
     2821 tcttcatccg ctgcagcgga aatttcttga gcacgcggcg ctgcagtgtg gcttctgtac
     2881 gccgggcgta ttggtcgcgg ccaaagcctt gctggaccgc aatccgaatc cgacggaaac
     2941 cgagacgcgc tactggctcg ccggcaacct ttgccgatgc accggctacg acaagatcat
     3001 ccgcgccgtt ctcgacgccg ccgccgaact gcgaggagta tgatatcttt tccgagtcat
     3061 tcattcggga gagatgcttt ttagtcggca cagtttggcg atgctagtgt gtgtatcgaa
     3121 aattagattc tgcaatattg gtggtgaagg ccgccgagaa ttgggtgtgg cgtgatggca
     3181 ccgaggcgct caatcgcgcg atataatggc gccactcaac cactgttgac caacgatacg
     3241 gcgcaaccaa acaagaacaa acgtcgtcaa acgtgatcga acgccacgct aatcatcaat
     3301 gttttccggc ttttccgttt cgatacgaca aggacttcaa aaagaagaaa catgggatga
     3361 ccagtttgaa aaatcaaaaa agcaggtcgg caaacgccac atagtggcca gtccctcagg
     3421 ggccgcgttc gctcggtgtc agcttgtggt atttagcgga caatccaaac gcagccgcgt
     3481 ttgttcgctg ctggactaaa gcgggcaaaa ggtaacctct ggccgtcggc ggtttgtccg
     3541 cttgtgaccc ggagtggaca ccccgcctta ggggacactg cacctaattc atcagacgaa
     3601 taggcggcaa catttcttca agggctgaaa cgccgccggc gcccaacgtg aagggcggcg
     3661 gcggcttcgg ctggttaggc gactgctatc tcggcggtca cggctttcgc cgcgcgatga
     3721 tcgctgccac ggcctccggc ttcttcaatt ccctgctatc agggaaaaga acagggcccg
     3781 gcattgtcgg gactggccaa ccaataccgt ggcccgccac catggggtgc caggcgggaa
     3841 cggcagtcgt ccgcgccgcc agcccgtctt gccagtgcgc ggaaaaaaaa gcaaaaattc
     3901 acacgaaatc gggagtcatc tggggaatcc cggataagat tgggccatgg gaatgcgatt
     3961 tcgacttggg gggtgaaccg atgaaaatcc tgcgccgcaa atggctgcat ttggcagcgc
     4021 tgagcgtcgt tctcgcgggc gtcgccgcga ttaatgagcc ggcatggccg cagaccggca
     4081 gacccattcg gatcatcatt ccgttcccgc ccgggggcgc cgccgacatt ctggcgcgca
     4141 tcctgagcca gcagatcggc gatgcgaaca atcagaccat gatcgtcgaa aatcacccgg
     4201 gcgctggcgc ctcgatcgcc tacgagctga cggcgcgcgc cacgcccgac ggcaatacga
     4261 tcgtggtggc ggcgaattcg ctcgtcatca atccgatttt gcgaaagacg aattacgatc
     4321 cggtcaccag ctatgagccg atttgcaatc tcgtcacgtc gccgatggtc ttcgtcgtca
     4381 acagcgtgtc gtcctacaaa acaatcggcg atctcatcgc cgcggcgcag gccaaacccg
     4441 gcacactcac gcttgctgcg ctcgggccgg ccaccacgca gcatattgcg gtcgagcaat
     4501 tcaaactcgt gacgaaggtc gacatgattt tcgtgcctta tccgggcggc gcccccgtga
     4561 tcaacgctct cctaggtgac caggtcacgg caggattggt caattactcg gaagcggttc
     4621 agcagatcaa agccggcaag ttgcgggcaa tcgcgaccgc ctcaaacaaa cgcatcgagc
     4681 cgttgcccga cgtgccgacc gtcgccgagt ctggctaccc gagttacgag tcggaagtct
     4741 ggctcggcct gttcgcgccg gccaagacgc cgaagaaaag catcgcgcaa cttatcgcct
     4801 ggtttaccgc ggcaatgaaa acgcccgtgg ttgaagcaaa attattgccg ctcggcctct
     4861 attcagcggt cgtctgcgga aaagatttcg gtgcgttcgt caaacagaga agcgacgagt
     4921 acgcacgcat cattcacgac gcaaacatca agatggagtg accatgagct tgcagcatat
     4981 cctcgtcgcc ctcgtcctgt tcaggctggt gaccaccggc tgggcgatcg ccattaatag
     5041 gtcctgagcc agtaagtcag agagaagcaa ctaccatact ctaccgcaag ggaagtcccg
     5101 aagatcccac gacgaatgaa gaactcatgg acaagtttcg gacgcttaca aagagacttg
     5161 atggtcggcg gcctgaacag atttccctaa tcgtatctca cattgaggat tgtgagaatt
     5221 tggcggaact ttcgcacctg ctgatggccg aggaattgat tgctacctag ccatcgtttc
     5281 tatgtcgagt gaggctcgcc ggtgttgagg cgcatggcgg cgtcggtggc gcggcgattc
     5341 cactttggtg tcagtctctc gatgaagtcg tacatgtagc cgggaagata ttttctctgc
     5401 cgcagaacga tatggcaggc cgcacaaatg caataatctc tccatcgtgc tggtacaaaa
     5461 tttccgtcat gtcatagtgg caaccctctt cgcgttcaaa ctctagcaat caattgaacg
     5521 ttcgcaggag ctggtcagta atcggcttaa ggagatagct cgtcatcgtg cgactgccgg
     5581 tctgcagaaa tacctcgacg ggcattccgg ccaccagttg taggctgcca agccgccgac
     5641 gctcgctact gggcagcgtc acccgcactg tatagtaagc tgcattggcc tggcggtcgt
     5701 ggctcaaatc ggccgagaca tacgacacga ctccttcgag ctgtggggtg gtgcgttgat
     5761 tgaatgccga aaagcgcacg taagcacgct gtccagagcc tacttgatca atatcccgag
     5821 gcggaagctt ggcttcgatc tgcaattcgt ccgattccgg cacaatttcc atggcgacct
     5881 cgccgggagc aataacgccg ccgacggtgt gaaccgaaag ctggtgaata accccattgg
     5941 taggcgcgcg caaatccacc cgcttgagca agtcctgtgc ggctacggtc ttctgaacaa
     6001 gttcggcctc tttatcctgc gcctcgcgca gatccttcat gacctcacta cgaaagtcct
     6061 gatcaatccg cacggcctgc agctccgcct cactgatctt ggattttgcc tctgcgatcg
     6121 ctgccgtcgt ttggccgcgg tcgccgtcga gcctagcggc ctcgcgctcc agcgatgttt
     6181 tacgtgtcag cggcaccagt cctttctggt acagactatc gacgccctcg agctcacgag
     6241 agatcaggtc gtgctgcgca gctttcgatt tcacctgagc atcgagaccg gaaatctgct
     6301 ccccgagttg cccgacatgg ctacgcaata gctcttttgc gctgcgacgc gccgccgcgc
     6361 ggctactgaa cagcgaaatc tccgatgccc ataggtgact gacattatct tcgcctaacc
     6421 gggtgctcat ttcttgcggc tttcgcggct gatccacgcc atcacgttcg gccgccaggc
     6481 gcgccagtct gacgcggacc tgatcgagct gttgcatcag aacctgataa ttggcttgca
     6541 gttgcgtgtc gtcgaggcga agcagcagat caccggcccg cacatgcatc ccgtcgcgca
     6601 caggaatata cgccaccacg ccgccagccg gatgctgaat cttcttcaca tccgattcca
     6661 ccacaagtgt gcccggtacg acgacggcac ccgatagcgg caccaacgtc gcccacccgc
     6721 ccaggattcc gacacccagg attaggacac gaagtcctat tttgagttcg ccctcaaaag
     6781 cgtgcgttag ccgcgctgtg agcccggcgc ctgacgtctc gtccagtcgg gtagcgcccg
     6841 cacgactgat caagaatgca caggcggagc gtaaatgttc aaacgcactg ccagaccgca
     6901 gaaactcaag gccggactgg aaaccgcggc ggaggctatt tgtggtctct gccggcgacg
     6961 gaatcggcga tggcctttta atcgaagagt aagcgctagc ctggtgcgtg tcacggcttt
     7021 cgatctttgc agtggttagc ggaggagtcg ccccttcagt gggttgcccc gcccgtggtt
     7081 gggaaaacac cttcacgcgt tggggccctg atcgccttgg tagcgccggt gttttgccgc
     7141 gctgcctcga cttgcttccg taaccaatag acttgcgccg attcaagccg atcattttga
     7201 ggacaaagtt gaccgcgccg tcgactgcgg ttcgagtcgg ttcgattcgg gtcccgcgcc
     7261 gattgtcaaa aagccagttg gccggccgcg cctcaacggc gtgcgtaaat tgctcgcgct
     7321 cttggtaact ttccttgtta agaaatttct gaaggcgcct cagttcgcaa atgagagcgg
     7381 ttccctgcgg cccaattccc gcatccgcgg cgttggtcgc gtcgtagtgt ccgagcgtat
     7441 ggctacgatc attggaccaa gtcatcgcgc ggcacctgcg accgcttgcg aggccccgct
     7501 ttgcttcggt gcttccgcgg aacgatcaac ggtccgcgcg acccgtgcga aaatttcctc
     7561 actcggcccg aacgctattg accgcccctc atgtattacc attgtcatgt tcaacactgc
     7621 caacgcgtta gaccggtgcg aaacgacgat gacgatactc tgctcgctcc gcagtgtttc
     7681 gatcgccttg acgagcgcat tttctccttc ggcgtcgaga ttggcatttg gttcgtcgag
     7741 tacgactaga aatgggcggc catacacggc ccgagctaga ccaacccgct ggcgctggcc
     7801 ggccgatagc gaggcgcctc gttctccgat ccgggtgtcg tagccgtccg gcagacgcaa
     7861 gatcatctcg tgaacgccgg ccacctgcgc ggcttccaga attgcctccg aggtcgcttc
     7921 ctcatcaaag cgcgcgatgt tcatcgctac cgtgccatcg aacaacgcta cgtcctgggg
     7981 taggtagccg atgtgtcgcc caagatcgtc gggatgccat cgatcgattg cggcgccgtc
     8041 gagacgcacg gcgcccgtcg ccgcgggcca gatgccaacg agcgccttcg cgagcgaaga
     8101 ttttccggcg gcgctggcgc cgagaagcgc tagtcccatt cccgcccgca ggccaaagga
     8161 cacttgggag aggactgctt tttgagtgcc gggcacgatc acgctgagat tctcagtcgt
     8221 caggatgcga tttggcctct gcaggacaac ggcgggggct gcgggcgccg cagtgacctt
     8281 tagtacagtg cgtaggcggt taatcccctc tcgagctgca acgagttgct tccagtttgc
     8341 cagcgcgact tcgaccggcg ccagggcgcg ccccataatg atcgaagacg caatcatgat
     8401 accgccagag gcctgctcta taactaccag ataggcaccg actccgagca ccgccgattg
     8461 cagaacgaag cgcaagacct tagccgcagc gctaaggttc gcatgcacat ccgcgagccg
     8521 cgtgttgtga tgaatgtagt gctcattcgt gagcgaccat cgatcggcca gacgacctgt
     8581 catgccgagg gcgcgaatga cctccgcgtt ctgacgcagt gcgtcggcca ggacctgccg
     8641 ctgggcattt ccctccatcg acattttcgc cgcgcttttc gattgacgct cagtcaggaa
     8701 cgtcatggcg atgatggccg ccgccccgcc ggtcgctatg acgccaatca ccggatgaaa
     8761 gaggaatagc gcaagcagga aaagcgggat ccacggcatg tcgagaaatg ctgtcgggcc
     8821 agcacccgac aggaatgcgc gcacctgatc gagatcgcgc agcggctgct gcgccagaat
     8881 tggttttgtg ccccgcagtg ccagagttac gagcgccaag tgaatccggg tctgcagttg
     8941 aacgtcaaac agggcggcaa tccgcgccag catacggcct cgaagcgcgt cgaaatagcc
     9001 ttgcagcaag taagcaacca ttactatcgc cgatagccca aacaatgttg caaggttgcg
     9061 actcggaatc acacgatcgt acacttgcag catgtagagc gagcccgaaa gtgtcagcag
     9121 gttgatcaca ccactgaaca ccgcgacccc cgctaatcgt cgtccgcttg cacgcaactg
     9181 aatcgcaacc ggatcgtccc cctcgagcga cagcatgctc ccccacaatg ctttcatgct
     9241 gcagcggcag tgtcccggcg ccacagcagt cggtcatgcg cctgccaatc gcgaatgctg
     9301 aacttcgatc gtgacaaaag ttcggcgggt ttgagattgt agaacgcgtg tccctgatcc
     9361 cgacggacaa tcttatttgc ataaggtttt tgcggttgac gcaatcggtt tcgaccgagc
     9421 gctagcgcct tctaattgcc tacgaatggt cacagtgtct gcctgctaat ttctcatttc
     9481 agtccgaacg agaccatgat tagccagcac catgtgacca ctcgaatttc acgtcgcagt
     9541 ttcattctca cttaaagcac taacacgggg gaaactcatg gcgcatgata cacaagggca
     9601 tcacggcgac accaacactc cgacgccacc cgaaaagcct catctgccga tgactttcgc
     9661 ggaaattggc acgctttaca attcaatatc cgatgaattg gagggtggtg tcacgcacca
     9721 aaaccagcaa ctactcctca atcaagtctc gactgtccaa acgcaacttc aaagcctgat
     9781 taacagcgga gccttgaata atctggacgg tgcaggaaac ccggctgtaa gtgtcgtcca
     9841 tgctcagaat gtcgccgatc aaatgaattt cttgaaaaca gagatcggcg cctttggtac
     9901 aggcaccttt gttcctaagt tcatcaacga cgtcgtcagg gatgttcaag acatcgtggc
     9961 cgccgacccg gcactgactg cactcgccca tcaggccaat cattccggtt tccaacaggt
    10021 ctcctatctc ctaacgaatc ccacgccgtt cgctgactca ggcgcacaaa cggacgccct
    10081 tcttaagttt atcaccgact cgaattcgct ggccgcgcgt gctcaggtgt tggccggcac
    10141 cgatcccaat tcagcaccgg tccatcagtt ggaaagcgac attcaagcgt tttccacggc
    10201 tgccgattct tattctacag aacaaggggg tctgttctcg gcccgtttca acaacgaatt
    10261 caccctcaat ggtgtccagg gaacggcctc gcgtgaaatg atcaccggcc tcgaaaccgg
    10321 caacgcagat ctcgtcaacg gcgcagcatc ggtgttgacg gcgaacgcca atgatgtgcg
    10381 gggaaatatg ttgacacaag gaaacacctt tacgcctgcg cctaatggcg gcatccccga
    10441 tttcattgac acagtgcatg acgctggggt cgtcttcaac gatgccatga ccaaactcat
    10501 tggcggcgtc tattccggta accagcaatc catcctcaat gatctgaacg ctaccgcggt
    10561 cggtctccat aatgctatca tggaccaggg gatcaccggg caggccctcc acgatatcaa
    10621 tcacgtcatc agtctcttgg gtcagcaagc ttccctggtg ggcggcatcg gcgccgcgtc
    10681 gcccactcag acctccgccg tcaatgggca aatcaatcaa atccaggcgc aaattctgaa
    10741 cgtcgtcaat catgacgcga ccctggcagc gatggcggtc ggtgccgatg gaaccgtcgg
    10801 cttcgtagcc ttgccgccgg ggacgacgcc aagccatagc gacgctgttg cttcggccgg
    10861 gcaccatttc gaacatatgt ggggctgagc caagacgcat ctatcgttgt ctgagcctgc
    10921 ggcaacatag ccgcacctcg gaatgcaagt gcgctcagtc gtacgcttgt tttgcggccg
    10981 gttgtggctc aaatcggccg atacgtacga cgcaactcct tcgagctgtg gcgtggcgcg
    11041 ctgattgaat gcggaaaagc gggcgggata cgtagaaagt tattccgaat ctggtcgaaa
    11101 tagctgggtg gggcatgagt aggtgcgctt cgcgtaaccg aagaactgac tacggcacag
    11161 ttatactgca ttggtgcttg gtcgtcgcgc tgacagtggc agtcctcagc gggcttagga
    11221 ttgccagcga gtcgcctgac cgctcatgga tcaatgctct tgatctcatc ctgccgcgcc
    11281 gcctcgtgtg gacaacgcat atgcaggccg cggtcgcact gatcccgatc gccatcgcct
    11341 acccaattta tgtctcgctc gcgcggctgt ggggacgcgt ccgattcgat cgaattcggc
    11401 taagcggcct attcggccgt catcaggcac gctggggcgc tgtcaacgtt gccctctatt
    11461 ggatcttcta tctcatgctg ctttcggaga tcgcgaccgg catcctcgtg tatttcgatt
    11521 acggcaacag caccataatc acacttcact ggctaggaat gtcgttcatt tgcggatgca
    11581 cgatcgccca cgttctggtt cattggaaaa ttggcaaggc gtcgcaactg ctgcgtattg
    11641 tgcgcccggc gcgtttgttg ccgcaacctc cgccgttcga tccaatcgac ctgttggaac
    11701 tgctcgatca acgggcttcc gagccgcagg aatctcaggt ttcgcgagca atgcacctcc
    11761 ggccggagct ttataacgat gcattacagc aatttgtgcc ggccaatgac catttcccgc
    11821 tgggccggcg ggagccgaag cagaaccttt tttaaaccaa agaaatacgt tcgcgagcag
    11881 gcacggcgga tcccgcggag tggatacaat gcttcaatcc aatcctttta ttgtggcgct
    11941 ggtggtggcg gtcgccggca cctcccttat tgtgacggct gaacgtcaga cgtcggaaag
    12001 cctattgatc cgccgcgtgg gtgcgcctga catcccagtc atcgacgggg acacgtccga
    12061 ccgagcgtgg caaatgacca agccgctcta tgtcctgacc gctcatggcg acaatttcaa
    12121 tggcaagggg gaaaccaccg tggaaatccg cgcggttcat gacggtcagt gggcctattt
    12181 tttgttcgtc tgggacgacc caacccgctc gttgaagcag ctgccattgt tgaaaacggc
    12241 ggagggttgg aaactccttc acgatggata cgaggcagga gcagaacatg cgtatagcga
    12301 agacaaattc tcggtcctgc tgaccaagct taatgtcatt ctggcgggcg atcgtacatt
    12361 ccatgccggt acccagccgc tagacggaaa gccgcgcact cttaccggtc gcggcctgca
    12421 ctacacggag cggcccggcc tcttcgcaga cgtttgggaa tggaaggcaa ctagcacaag
    12481 cccagccgga ttcatggacg atgatcattt cggtccacca gtcgaggcca caagtgcgca
    12541 ggctgaaggc aaaatcccct accgtggcgg attcgctgcc gatcctggca ctgccagtta
    12601 tgttgacaat ttcgcggttg aagacccgaa cgactaccgc aagttcgtca ctcccctccg
    12661 cctgcccaaa gacgtcagca cgatgacgac cgcgttgggg gtgatcgatc tcgaccccaa
    12721 ccatggcgag ggtgtcgacg cacgctggtt tatgactatg gaggaatcga cgccctattc
    12781 gcgcgacctc gatgcaaaga ttcccgtcgg cactatcgtt ccaggcgtca ttttctccgg
    12841 tacttattcc ggggaccgcg ccgatgttcg ctgcgccgcg cgctgggctg ccggacgatg
    12901 ggcactcgaa gtggctcggc gactcgatac cggaagtccc tacgacgtac ccatcagtac
    12961 gggaaccttc atgcgggtag cggccttcga ccatacgcag atcggtcaca caaggcatgt
    13021 tcgtccgata cgtttggagg tcgaataatg ggcaaagtgt gcagcctgga ggtgaatggc
    13081 aattcgatct ctgtgcaccg cggcgatctg ctgctcgacg ctgcgctact gaatggcatc
    13141 gacatcccgc atgattgccg gtccggaaat tgcggcacct gtcgcgtacg cctgctcgac
    13201 ggccgcgtgt ttggtggaca gacagacgat ccggagatgg tctatgcctg ccagtcccga
    13261 atcgttacgg atctgcgcgt ccatgttgag gacgtgcctg aaatcgtcac gatgccgggc
    13321 gtggtcgccg atctcatccc attggcgcca gatgtgtgcg aagtttgcat cgaaacgcgg
    13381 aggccggtcg aatactttcc cggtcagtac tacgcggtgc aattccgcgg cttccccgct
    13441 cgctacttca gcccgacttc ggcgctcgac tggccgtgca acgaagcact cgtgcgattt
    13501 catatccgaa ttgtgccaaa cgggcggatt tcctccgcgc tcggtcaacg tattggctcc
    13561 ggacaccggg tcaagctgga gggtccgttc ggttcggcct atctgagggc atttcccgac
    13621 caacgccttg tgttggtcgc gagcgggagt ggctttgctc caatctggtc gatcgccgaa
    13681 actgcgatca agagtgcgcc gcggcgcgaa ctcgtgctca tcgtcaccgc acgatcgttg
    13741 gactctctgt atatgatccc tgcgctttgt cgattggcgc tgttccccaa tgttgtcatc
    13801 attcccgtgg tttcggaaaa tcaggatgtc acggcggcgg tccgccgcgg caagccgacc
    13861 gatcacctcc ccaacttgac gccgcgggac attgtcttcg cggccggcgc cccggaaatg
    13921 gtccgcgccg tcgcgcggat gtcaggcgct gccggggcga aatgctacac cgatccgttc
    13981 gaagcggcat cgcaccgcac ggcgaccgat ttgtggtcgc ttgccgtgag ttggctcacc
    14041 aacacctcag gtagaaggtg agggtaggaa ttttggtacg ctgttcagag ttacgaaaag
    14101 ctccgaaact tcaaatgaaa taagcggatg gcttctccgc ccgcctgcaa ctctcgcacc
    14161 aacgggtgtc gggagccaac tggctccgat aagcgcgatt atcgatgcca agctgccgag
    14221 ccagcctaag cactggcgga tcgctaaaga aggtcgtcta ttgtcggaga gcgtacttct
    14281 tcgaaaacct gtgtgtaccg aaaaccccat ccgcgatgat ttcaattttg aacgacaaaa
    14341 ttccctgttc ttgcggcaga attccctgct ccgccgaaaa aattccctgt ttcattgagt
    14401 agggggtata gtgatagtga agaaagtagg ccacctgcta agtgcagatc atgtcgtagc
    14461 gttgattttt tatttgaaat tgaaaatgag attagcccgg gcttttgttt accttgccca
    14521 gtgatacgca gggtctttac agtcattgcc ctccgagaat gcgaccggct cgcgttcttt
    14581 accccaaatg ttttttccgg cgcgttgcgg cttaagtctg aaattagtgt tccgctctag
    14641 gaaatactca tcgatggctt tacgacattc ttcaagcgac acataatcgc tgttgtgaat
    14701 aatcgctctc gccatgccac tgaaaataga ttcaataatg ttcaagtatt gaccgcttgc
    14761 tggcagcaac aaactctcaa tcttaggcca cttaccaccg ttgttgtgat cgataaaagt
    14821 atcgatcgcg tctgacttat gccaagaggc cgcgtcccac gaaatccata gtttccgctt
    14881 gcgtctatac tttttgcgaa gtagccgaag catcttgatc atttcggcgg agtttttacg
    14941 gtcactgtag aaatgtgtca cttgattgct tgatagttcg attgctgcag tcaggattat
    15001 caatccccga gatttctgac gttgttgcac cacgggtgtc ttgcctggag caacaattga
    15061 acgccctccc atctttcgaa cgtggaaggg accaaactca tcgatcgaaa agagtgcttc
    15121 gtcatttctc aattgcgagg cgacctcacg gacgcgtgcg accttttcct tgtagaaggt
    15181 atcattagaa gtcaacacaa tccgcgcctt gcgccagcta taaccagccg atttcagaat
    15241 ctgtctgatc acatcggcac aagctggatt cccgtcattg ataagagcct cccggagcgt
    15301 ctgcattgac cacgccgccc ggttaaaccc atgatctcgt ggcggcgcat gcaagattct
    15361 aaatacactg cgccgaagcc cttcttcttc gcgccttcgt ggatgcccag ccggaggggc
    15421 caacatcgcc ttaacgccgt cccgatcgca aatcttcagg tttttatcga caaaactttt
    15481 gttcattcca gcaaagctgc aaattgtagt ctttttgatc cctttgcgat gggcaagaaa
    15541 agcaattgct ttcatgcggt tataatgttt tccatttcga agcgtcgcca tgagctgctt
    15601 caaatcaggg atgtcgccaa catcctcacg aacctcttca atttttaaaa gtccaaatgc
    15661 gagcctgcac ttccactcac tatcgagccg ccgcgcagaa tctcgagggt ccatccgcca
    15721 ttcaacattt gttataagct tacaagcctg taagaggctt gctgcgcggg tttgattctc
    15781 ggtgtctgct cgatacaaat aaaagagaaa acgtactgct ctatcgcggt cgagcgcact
    15841 taagacttca tagagttctc cccagaactg atatcttctt cggctaaggt cgcccaaatg
    15901 gtttttgagc gttttttcag atccatcgta tccatcttgt cgaatcctct cgagaatttc
    15961 ctttgcatgt tttggtgtag gaggcggccg gtctgtccga cgggtcaaca ttttctctat
    16021 tgtgcttagg tatgggccga tcttagagcg tgaactccga tcccgaattg gagatggcgg
    16081 cgtcgggtgt gccagtattt tatgaagtgt ggcgtagctt aacttctgct ctcgcccaac
    16141 ttggcgtcca gagagcccat caataagcac acggttccgg atagtggtcc acctcagttt
    16201 atcccggtac atggcccaag cccttaccct gaaagtgaag ctccccaatc ctcacgtaaa
    16261 ttctgcaatt gccttatgga ccctcaactc ctgtgatctt tcattgtccg ttcagcgtgc
    16321 cgcgattgcc attatcatcc gcctctttct tgcatacgta ggctccattc tttgtgtggc
    16381 cgtaccagcg catgcctttc cagtgataga tgtgggtagg tagattgagc cagacaacga
    16441 catcgttggg gcaatgctgt tgcgcttgat ccgccgaaga aaaaagcgtg agtgaagcaa
    16501 tcgccggaaa ggttgagcca attatgaggg tcgctaaaac gacaagctta ataggtagag
    16561 tttttaacat acaagacctc tgtgcgtgca aagcatctgg gcattggcgg ccgcgcgttt
    16621 ctattggcac gcgctttcgt agtcagagac tgccgattcg aagtcgtctt gtgctgaatg
    16681 cagcgttaag aactctgttg aacagtcgtc atgtccatca ctgccagaaa tacagctagc
    16741 atagattctc agcgcgtcag atacgtcgct ccttgccgaa ttatattggt ctactgcatt
    16801 tcgacattcc tcgggatcgg cttttgtggc cgttatcgaa attagcaata gggcgagtgt
    16861 gaaacaaatt acagcgaatt gttttcgcat gatcggcatc cctcagattt agccaacctc
    16921 ttgttttaat gatgctgttc aaagaacatt gtgtatttac tgccactcga tctattggcc
    16981 gaatagctag cgtaaggctt ttttgattcg tttaagccga agcgcatcgc acgtccgcat
    17041 tacccctgcg atcaattcga aagcgcttgc ctggattcaa cgtcgtccag gaaattgcgg
    17101 accgcagcgt tgaaaatgat gggctgctcg atattggata tatggccggc ctgattgatc
    17161 tcaaggaaac gcgcgtcttt gatcatcgcc tgcatctggc ggctgtttgc cggcggcgcg
    17221 ccatggtctt cgctgccgca aatcacaagc gccggagcat tgatttcctt cagcctggag
    17281 ccgtagtcga gccgcgctaa tgcggccgca cagccgcaat agccgttggc ggatgtcttg
    17341 cggatcatgg aatccatctg tgcaatcgcg gctggatttc gctcggccaa tccggcgctc
    17401 gaccagcggg ataccgtcga ctccacgatt gcctctatcc cttgcttgcg aacggcatcg
    17461 gcgcgctgaa gccatgcttc ggtaaattcc ggcgtggtcg tatgccggga atcggcgatg
    17521 accgcgctct tcacacgctt cggatgattg ataagcatgc caatggcgat catgccgccc
    17581 attgaaaggc cagcgacgtg gcattgctcc acgccaagcg cgtccagaat gccgacggca
    17641 tcggcgacaa gcatatcaag cgtgtaatcg cctgccggtg cggctgaccc gccatggccg
    17701 cgcgcgtcat atcgcagcac tcgataacgg tcgctgagag cttcgacgag ttcgtcccac
    17761 atcgacatgt cggtcgccag gccatgactc agcatgaccc agggaccgcg atcgccgtcg
    17821 gtctggcaat tgaacgacga cccattgacc tgaactatca tgagaagcct ctttcagcat
    17881 tggcgttgac tcgcgccaat atgcagtaac tgggccgtta cgggtaggga aatgggtaag
    17941 tcccgcgagc agagaagcac acggcctctg tttgctgaag ccgcagagaa ttgccgcgct
    18001 ctcgcggcac caaatcgatt ccgggccgcg ttatcgccgc ccaatccggc ttctagatcc
    18061 ctcgacagtg gtttcaatgg cttgctagtg tcgtgttcac aagatgccgg ggaggtcgct
    18121 gtgaaaagag agaaagccgc gttgcgatct gttgccgaat ttcggatcgt ttcccttgcg
    18181 atcgcaattg ttgcggtcat ggtgggtctg gcgtctccgg caagagcgca ggcggattat
    18241 ccaaatcgtc ccgttcaact cctcatcgcc tacggtgcgg gcactattgg cgacgtgagc
    18301 atgcggatct tggcccagaa gttgagtagc gaactcggca agagctttgt tgtcgaaaac
    18361 cgtcctggcg ccgctggtgt tattgccgcc aaggcagccg cggcggcagc ccccgacggt
    18421 tacacgctgc ttctgacggg caacagctac gccataagca ccgcgctctt caaatctttg
    18481 ccctatgacg tgcttaaaga ctttgcgccg atatctaccg tcgcaacttt cgattttctt
    18541 gtcgcgacca ggaaagattc aaaattcaag tcgatgaagg atgtcattgc ctatgcaatg
    18601 gcaaatcccg gaaagctgaa cattgccacc ttgagcccag gcaccaccca atatcttgcc
    18661 gtcgagttga ttaaagtcgc cgccggagtc aacgtaaccg ccgttccgtt ccgtagttct
    18721 cccgacgcgg tggccgcact gctgcgcggc gacgtagaca tcgatatgga ctcctacgct
    18781 ccgctaaagt cgttactgga ggccgacaaa attggtgtta ttgccaccac cggccgcaaa
    18841 agggttggtt tcttgaccgg ggtgccgacc gtgatggaaa gcggcctgcc aaatttcgac
    18901 gtatccagtt ggaatggtat tgccgctccg gccggcgtgc cggcgccgat cgtcgcgaaa
    18961 ctcagcaaag ccgtgaacga agcgcttcag tcatcggaag ctcaggcgac cggccgcagg
    19021 ctcggtatgg agatgcgcgg cagcactccg caagaactgg aggcgcgatt aaaaggcgat
    19081 atcgccaaat ggtccgacct catcgagaag gcgcatatcc caaagcacga ttaaagcggc
    19141 agacggcttg cggcaggagt tcgaacgctt cgggcgcgcc gtcaagttat tgatatattg
    19201 attgttttat aaaccgcgac gctctcaata ttctggcttg taaactagtc tggtgccatt
    19261 cgcgtcccaa gcgagatctg tcggcacgcc aatgtgaata ggatatctgc aaatccgccg
    19321 cgtattgggc gccgaatatc ttaggatcgg ccgggataag cgcagcaccc ggcgccacag
    19381 gagatgacga atgaaaattt tggcagcaat tggttcactt gcaattgttc tcgcggtcgt
    19441 cgctgcagtc tatttcttcg gaggcttcta cagcgttgcc ggcagccaag aagatcccgc
    19501 gatcctccat tgggcgctgg tcaaggttcg gaccaattcg atcgttcgcc atgcgaacga
    19561 tcagccgccg gccggtttcg acgatcctgc caaggtacag gccggcgcac gcgaatttgc
    19621 cgagcacggc tgtgtcaatt gccacggcgc ccccggcgtt aaatgggcca agttctcgga
    19681 aggcttgcac ccggatccgc cggacctcaa ggaggtcgtt gcagaggcca cgccgtcgca
    19741 gctgttctgg gtgatcaaga acggcatcaa catgactggg atgccaggct tcggagccgc
    19801 cggtgcgacg gatgaagaca tctgggccat agtggccttt ctacagaagc tgccgaacgt
    19861 ttccgaggcc gactacaaaa cctggaccgc gccggcacct gcgccgccct caaaaccgta
    19921 gcgccgggta cgacactagc gatgcaggca ggcatggacc cgaattaggc gccgggcagc
    19981 ggccggaatg gcggtgctgt tcagttcgct ctgcgttgcg gttttgcagg aaagccaaaa
    20041 gtagcccgcg tccggatcga ccacgagacg ggtaggtttg aggcccggcg ccacgagcac
    20101 cgataggtag ggggtcgtcg aattccaagc gagccggttg aggccggcgc caatccgttg
    20161 cgacgaaacg aattgcgggt cgccaagagc cggatcggag tccgtgcctg tagcgagcgc
    20221 gcgcaccgcc gctttgtagt ctatccacgc cccggcgaat ttcgtcgtct cgacggcata
    20281 aaccagcatc gtcagcagga ttgcccccga aatcatccgc ggattcgcgt gcatgccaat
    20341 tgtgtccgcc aactgcgcca agaacggaaa cgcagacttt cgccggctct catcgtccat
    20401 tgtctgcacc g
//
LOCUS       DATMGX010000674         2427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_359146, whole genome shotgun sequence.
ACCESSION   DATMGX010000674 DATMGX010000000
VERSION     DATMGX010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359146"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VNR65_11065"
     CDS             <1..1169
                     /locus_tag="VNR65_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWJ19257.1"
                     /translation="RAGNSRELQPTIPQGVKTRRTAIRESGKFMRKILPTSWTRFFGL
                     AAIGTALIGSVVILVIESMPTRSEQAVRPDAAWAAAAPGRVEPKGRELRIVAPGPAVI
                     KEVLVGLNDRIQKGDLLIRLDDEELKAKLAAAKAQVAVRTTDRDNVKASDAALDRRKA
                     EDNLYNAERGAFDARMDLDHLISEARGGKVSLGDVQNGRAAVAAADEKVLQDQQKLKK
                     VLAKDMPALKREEAALSAARADVSGVSASLERMRIRAPIDAAVLALNAKVGEMAGASA
                     ENPLLTLGDTVRLQVRAEVEERDVGKIYQGQSAVVKSDAFPNRTFEVRVATIAKALGA
                     PQFSSRNRRKQADVDALEVVLDLEQGVPLLPGMRGDVLFREAATAPKSSSAGLQ"
     gene            complement(1174..1623)
                     /locus_tag="VNR65_11070"
     CDS             complement(1174..1623)
                     /locus_tag="VNR65_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19258.1"
                     /translation="MFEVARRQPDAALTNITPVSANKFRFLFIWPGSNPAMTYDRVGR
                     SFAERFAYFAEQWKLLAAGFCLSGANLAFGTTSYGEQDVNIAYRDIEVRYAMGKQANC
                     EGRYISLAEVEKTLKSSSASNGYSIGALPAAPEEALKPFLTPLTPSE"
     gene            1965..2312
                     /gene="rplS"
                     /locus_tag="VNR65_11075"
                     /pseudo
     CDS             1965..2312
                     /gene="rplS"
                     /locus_tag="VNR65_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
BASE COUNT          524 a          694 c          753 g          456 t
ORIGIN      
        1 aaagagcggg caattcgcgc gaattgcagc cgacaatacc gcagggagtg aaaacgcgcc
       61 gaacagcgat cagagaaagc ggcaagttca tgcgtaagat cctacccact tcctggaccc
      121 gtttcttcgg cctggccgcc atcggaacgg cactcatcgg ctcggtcgta attcttgtca
      181 tcgagtcgat gcctacgcgc tcggaacagg ctgtcagacc cgacgcggca tgggcggcgg
      241 ccgcccctgg ccgcgtcgaa ccgaaggggc gtgagcttcg catcgtcgcg ccaggccctg
      301 ccgtcatcaa ggaggttctc gtcggtctga acgaccggat tcaaaagggc gacctcttga
      361 tccggctgga tgatgaagag ctgaaagcga aactggccgc cgccaaggca caggtcgcgg
      421 tccggacgac cgatcgggat aatgtgaagg cctccgacgc agcgctcgac cggcggaagg
      481 ccgaggataa tctttataat gccgagcgcg gcgctttcga cgccaggatg gatctggatc
      541 acctgatttc agaggccaga ggaggcaagg tttcgcttgg cgatgtccaa aacggccggg
      601 ccgctgtcgc tgccgccgac gaaaaagtgc tgcaggacca acaaaagcta aaaaaagtgc
      661 tcgcaaagga tatgcccgcg ctcaagcgcg aggaggccgc gctgtcggcc gcacgcgccg
      721 acgtttccgg tgtcagcgca agccttgagc gcatgcgcat ccgtgcgccc atcgatgccg
      781 ccgtcctggc gctgaatgca aaggtcgggg aaatggcggg agcttccgcg gaaaacccgt
      841 tgcttacctt gggggatacg gtccgcctgc aagtccgcgc cgaggtggaa gagcgcgacg
      901 tcggcaagat ctaccaaggg cagtcagccg tcgtgaaatc ggacgcattc ccgaaccgga
      961 cgttcgaggt gcgggttgcc acgatcgcca aagcgcttgg cgcgccgcag ttctcatcgc
     1021 ggaaccggcg caagcaggcc gacgtcgatg ccctcgaggt tgttctggac ctcgaacaag
     1081 gcgttccgtt gctaccggga atgcggggtg atgtcttgtt tcgcgaggcc gcaactgctc
     1141 cgaaaagctc gtctgcgggt ttgcaataaa tacttactcc gagggagtga gaggggtgag
     1201 gaacggcttc agcgcctcct ccggcgcggc cggcaaggca ccaatgctgt atccgttgga
     1261 cgccgaagaa gacttgagtg ttttttcaac ttctgcaagc gagatgtacc ggccctcgca
     1321 attagcctgc tttcccatag catagcgcac ttcgatgtca cggtaggcga tgttcacatc
     1381 ttgctcgccg taggaggttg tgccgaaggc gaggttggcc ccggatagac aaaagccggc
     1441 cgcaagaagc ttccattgct ctgcaaaata ggcaaaacgc tccgcgaagc ttcgtcccac
     1501 ccggtcgtaa gtcatggccg ggtttgagcc gggccaaata aacaggaacc ggaacttatt
     1561 agcggaaacc ggggtaatat tcgtgagcgc ggcgtctggc tgtcttcgcg cgacctcgaa
     1621 caactcgttc atgctcttcg ggtcgagccc atttataatc tctttcgccc ggtctccggc
     1681 ggatgccacc gagaccggga cgcataatgc caataagctg gcggcaaagc ttttaagtgt
     1741 gagttgtcgt atcatggtgc cgatggtgcg tttaagtgag cctattcgtc aacatattcg
     1801 ctgaaacttg cgcggaaatc gggcaggtag cgaaatgttg caatcgacaa gctcactttt
     1861 ttgccttaag taatcgcaat tccagctcaa ctggcgcggc gggcttgcgc tgctttttca
     1921 gcgcggcata aaggcccttt gatgtgccgc agaggaattc aggtatggac atcatcgaga
     1981 cgctcgaaaa agagcaaatg gcggagcttg aaacgaagcg ccgcatcccg acctttcagc
     2041 ccggcgacac cctgcgcgtc agcgtcaagg tcgtcgaagg cacccgcgag cgtgtccagg
     2101 cctatgaagg cgtctgtatt gcccggtccg gcaggggctt caatgaaagc ttcacggtgc
     2161 ggaagatctc ctacggcgag ggcgtcgagc gcgtgttccc gctttactct ccgctgatcg
     2221 acagcatcac ggtcgtgcgc cgcggcaagg tacgccgcgc gaagctctac tatctgcgcg
     2281 gcctgcgtgg caaagcggcc cgtattcccg agcgcacgga caaccgggcg aaggcgctaa
     2341 aggcggagtt caaaggcttc aagcggccaa ggggcaatgt tgacaacctc aagcttatca
     2401 aggggcttga ggccaacgac gagaagc
//
LOCUS       DATMGX010000675         3434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_107311, whole genome shotgun sequence.
ACCESSION   DATMGX010000675 DATMGX010000000
VERSION     DATMGX010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107311"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNR65_11080"
     CDS             <1..210
                     /locus_tag="VNR65_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19259.1"
                     /translation="VYVLDDRTQKIPLANLDVPATVKTNDDRGMEFKIPPGQHPAQKK
                     NSDLQPNGTPDQNPPPAANVASIMP"
     gene            756..3398
                     /gene="gyrA"
                     /locus_tag="VNR65_11085"
     CDS             756..3398
                     /gene="gyrA"
                     /locus_tag="VNR65_11085"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWJ19260.1"
                     /translation="MADDPNQQLPLDPTRPGGGNVQSVNIEEEMRRSYLDYSMSVIIG
                     RALPDIRDGLKPVHRRILYAMHDMGVLHNRKHMKCAGVVGECLKKYHPHGDTAVYDAM
                     VRLAQPWSLRYPLIDGQGNFGSVDGDPPAAYRYTESRLTEIAEELLSDIDKDTVDFVA
                     NFDDTTVEPMVLPTRIPNLLVNGSNGIAVGMATNIPPHNLTEIVDACITLVNNPHATL
                     ADLLKHVQGPDFPTAGFIYGRSGITEAYRTGRGRFMMRAKAAIEHLTKDKDAIIVTEI
                     PYQVNKARLIERIAELVNNKVIDDISDVRDESDRDGMRIVIELKRSAEAQIVLNQLFK
                     HTSMQESFSMIFLAVVNNQPKEMGLVQAIQHFVDHRIDVVRRRTAYLLMKAREREHIL
                     EGYQVALDRVDDVIRIIRGSENRAEARTNLVDYFSGKTVNVSESNANRQAGKPLALKG
                     LVPSQLTRPFDTVQADAILELQLHRLTRLSIDEILKEMAEVRGRIAEYESILASEKKL
                     RGLIVKELEEVKKKYGDARRTIIQDEAAEISLEDLIADEQVAVTVSHSGYLKRTPIST
                     YRQQRRGGTGRKGMSTREEDFVEYLFVASTHDYILVFTNTGRVYWLKVYEIPELGAAG
                     KGKAIPNLVSLQPGESMRALLTVRDLEEEGKYVFFATRSGTVKKTPLKDFSNVRSIGI
                     NAIAIEKEDELVAASCTDGSQIVFLATHDGKAIRFDEEDVRPMGRQAHGVRGMNLGKG
                     DYIVGMAVTPKARGKAKNGDEQEASLILSVTEQGYGKRTDVDEYRLQTRGGKGIINVK
                     TTSRNGKVVSIMLVNEKSEVMVISQFGKIIRIGTKTIRECGRSSQGVRLLHMEEGDRV
                     AATSVIPPEEVVENGNGESRLLLQ"
BASE COUNT          803 a         1014 c          994 g          623 t
ORIGIN      
        1 gtttacgtcc tggatgaccg cacccagaaa atcccgctcg ccaacctcga cgtccccgcc
       61 accgttaaga cgaatgacga tcgcggtatg gagttcaaga ttccccccgg ccagcatccc
      121 gcgcagaaaa aaaattcaga cctccagccg aacggcacgc ccgaccagaa cccgccccca
      181 gcggccaacg tggcttcgat aatgccctaa ggcagtttca cacttggtgt atagggtgga
      241 gcaccgcttc agcggtgcag tttgagattc agaaatgggc cggctttagc cactgaggtg
      301 acggaaacat tacctcaggc gctaaagcgc gatccaggga ctgacggccg ttatgcacgc
      361 ctgaaaggcg tgctccaccc atcgcctata caccagatgt gaaactgcta taagggattt
      421 tccacccaga tcgctattgc ccgcaattag cttaattcca gcgaggcgac gaggggtcta
      481 ccctaccctc gaatcccgcc accaacctca tgtaatcctc tgaaaagagg tggtttaaca
      541 tgatccaaaa gcatgtctgc agtgttataa tttgaagtgc tcttcgaacc aataatctcc
      601 tgaaatcgca atgatttaca tgcaacccgg gcggaactcg cagagagaac ggcagtgccg
      661 cttctgcgtc caccaccaaa gcaaatcgcg ggcccttgtg aagcatagat gccgcgtaaa
      721 ttcgggaggc taatggctaa tagctaaagg caatcatggc agacgatccc aaccagcaat
      781 taccgctcga ccccacgaga cccggcgggg gcaacgtgca atccgtcaac attgaagagg
      841 agatgcggcg ctcgtatctc gactattcca tgtcggtcat cattgggcgc gccttaccgg
      901 acatccgcga cggattgaag ccggtgcatc gccgcattct gtacgccatg cacgacatgg
      961 gcgtgctgca taaccgcaag cacatgaagt gcgccggtgt ggtgggcgaa tgtttgaaga
     1021 agtatcatcc gcatggcgac accgcggttt acgacgccat ggttcgcctg gcccagccct
     1081 ggagcctgcg ctatccgctg atcgatggcc aaggcaactt cggttcggtc gatggcgatc
     1141 cgcctgccgc ctaccggtat accgaatcgc gcctcaccga gatcgccgaa gagctgttgt
     1201 ccgatatcga caaagacacc gtcgatttcg tcgctaactt cgacgacacc acggtcgagc
     1261 caatggtgtt gccgacgaga atccccaacc tgctggtgaa tggatcgaac ggtatcgccg
     1321 tcggcatggc gaccaacatt cctccgcaca atctgacgga gatcgtggac gcctgcatca
     1381 cgctggtgaa caacccgcac gccacgcttg ctgacttgct gaagcacgta cagggccccg
     1441 atttcccgac ggctggcttc atctacggac gcagtggcat caccgaagcc tatcgtaccg
     1501 ggcgcggacg cttcatgatg cgcgccaagg ctgctatcga gcatctcacc aaggacaagg
     1561 acgcgatcat cgtcaccgag atcccctacc aggtcaacaa ggcaaggctg atcgaacgca
     1621 tcgcggaact ggtgaacaac aaggtcattg acgatatcag cgatgtccgc gacgagagcg
     1681 accgcgacgg catgcgcatt gtgattgaac tgaagcgtag cgccgaagcg cagatcgtgc
     1741 tcaaccagct cttcaagcac acttccatgc aggaaagctt cagcatgatc ttcctggccg
     1801 tggtcaacaa ccagcccaag gagatgggcc tggtgcaggc catccagcac ttcgtcgatc
     1861 accgcatcga cgtagtgcgg cggcgcaccg cttatctgtt gatgaaggcc cgagagcgcg
     1921 agcatatcct cgagggctac caggtagcgc tggatcgggt cgatgacgtc atccgtatca
     1981 tccgtggcag cgagaaccgc gccgaggccc ggaccaacct ggttgactat ttttcaggca
     2041 agaccgtcaa cgtctccgag agtaacgcga accggcaagc cggcaagccc ttggcgttga
     2101 agggcctggt tccgtcgcag ctcacgcgtc ccttcgacac tgtgcaagcc gatgccattc
     2161 tggaattgca gttgcatcgg ttgacccgtt tgtcgatcga cgaaatcctc aaggaaatgg
     2221 cggaggtccg cggccgcatc gccgagtacg agtcgatcct cgccagcgaa aagaagctgc
     2281 gtggcctcat tgtcaaggaa ctcgaggaag tcaaaaagaa atacggcgac gcgcgccgta
     2341 ccatcatcca ggacgaagcc gccgagatca gcctggaaga cctgatcgcg gacgagcagg
     2401 tcgcggtcac ggtcagccac tcgggctacc tgaagcgcac tccgatttcg acctatcgtc
     2461 agcagcgtcg cggcggaacc ggacgcaagg gcatgagcac gcgcgaggag gatttcgtcg
     2521 aatatctctt cgtggcgtcc actcacgact acatcctggt gttcaccaac acgggtcgcg
     2581 tgtattggct caaggtgtac gagattcccg agctgggcgc cgccggcaag ggcaaagcca
     2641 tccccaacct ggtgtcgttg caaccgggcg agagcatgcg ggcgttgctt accgtgcgcg
     2701 acctcgaaga agagggcaag tacgttttct tcgccacgcg cagcgggacg gtgaagaaga
     2761 cgccgctgaa ggacttcagc aacgtgaggt cgattggcat caacgccatc gccatcgaga
     2821 aggaggacga actggtcgcc gccagttgta ccgacggcag ccagatcgtt ttcctggcga
     2881 cgcacgacgg caaggcgatt cgcttcgatg aagaggacgt acggccaatg ggtcgccagg
     2941 cccatggcgt tcgaggcatg aacctgggca aaggcgacta catcgttggc atggcggtca
     3001 cccccaaggc gcgcgggaag gccaagaacg gcgacgagca ggaagcgagc ctcatcctct
     3061 cggttacgga gcagggttac ggcaagcgca ccgacgtcga tgaataccgc ctgcaaacgc
     3121 gcggtggcaa gggcatcatc aacgttaaga cgacctcgcg caacggcaag gttgtttcca
     3181 tcatgctggt gaacgagaag tcggaggtca tggtgatcag ccagttcggc aagatcatac
     3241 gcattggcac caagacgatc cgtgaatgcg gacgctcgtc gcagggcgta cgtctcctgc
     3301 atatggagga gggcgatcgt gtggccgcca cctcggtcat tccgccggaa gaggtcgtcg
     3361 agaatggcaa cggcgagagc aggttgctgt tgcagtagtc aagagtaatt aggagcggcc
     3421 ctcaatctgg atta
//
LOCUS       DATMGX010000676         2649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_463423, whole genome shotgun sequence.
ACCESSION   DATMGX010000676 DATMGX010000000
VERSION     DATMGX010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463423"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..83
                     /gene="rrf"
                     /locus_tag="VNR65_11090"
     rRNA            <1..83
                     /gene="rrf"
                     /locus_tag="VNR65_11090"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(162..1244)
                     /gene="apbC"
                     /locus_tag="VNR65_11095"
     CDS             complement(162..1244)
                     /gene="apbC"
                     /locus_tag="VNR65_11095"
                     /inference="COORDINATES: protein motif:HMM:NF008669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HWJ19261.1"
                     /translation="MTETLTKDDVLKALKSVKAPNGQDIVTLGILSEIVINAGKVYFA
                     LTVPQRDAKAYEPVRKSAETVVKALPGVAGAMVTLTADTKEVPEKGASGQGIGGIKHI
                     IAVASGKGGVGKSTTAVNLALAFRENGLRVGVLDSDIYGPSVPRLLAIKSRPELIGDN
                     LLAPIEKYGLKVMSIGFLVEEETPMIWRGPMVISALTQMLKDVAWGELDMLVVDMPPG
                     TGDAQLTMAQQVPLSGAVIVSTPQDLALIDARKGLNMFKRVNVPVLGIIENMSYFLCP
                     KCGHRSDIFSHGGARHEAERLGVPFLGALPLHMAIRETSDSGHPIVAADPSSMNAKVY
                     KTIAEALWQRLSSEDATRQLPSIIIE"
     gene            1518..1712
                     /locus_tag="VNR65_11100"
     CDS             1518..1712
                     /locus_tag="VNR65_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19262.1"
                     /translation="MTIGDYSDLLAAGMIKSSGEMRRELDTVVVRNGLHDQFRGETQE
                     EVPTSGLCERHSFPRSYGRN"
     gene            1909..2223
                     /locus_tag="VNR65_11105"
     CDS             1909..2223
                     /locus_tag="VNR65_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWJ19263.1"
                     /translation="MALNNIAAHDKLIYTIGRLASEIVIKTVKIGLLILASRSSFTAW
                     GVELARRSGLTLIGRMLGKRFVALSGEVPILYDHDVMPLRMRMHGIEARAPGMKAHKW
                     NR"
     gene            2211..>2649
                     /gene="mobA"
                     /locus_tag="VNR65_11110"
     CDS             2211..>2649
                     /gene="mobA"
                     /locus_tag="VNR65_11110"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HWJ19264.1"
                     /translation="MEQVAGVILAGGLSRRMGGGDKSLRTLNGKPLILRAIERLGGQA
                     GPIALNANGDASRFSAFGLPVIPDATADFAGPLAGVLAGLRWAAEAAPDARFIVTAAC
                     DTPFFPSDLVEKLLASTGPTYPCIALAASGENAHPVFALWPVAL"
BASE COUNT          567 a          774 c          725 g          583 t
ORIGIN      
        1 cactcgatcc catctcgaac tcgaccgtga aacgttcccg cgccgatggt actacactgt
       61 gggagagtag gtcgtcgcca ggcatacaaa ggatgtttaa atccccccct cacccttaaa
      121 ttaaagcccc gtccctacag acggggcttt ttttgtgtct gctattcgat gatgatcgaa
      181 ggtagttgcc gggttgcatc ttcgctgctg aggcgttgcc acaacgcttc agcgatggtc
      241 ttgtaaacct tcgcattcat ggaagatgga tcggccgcga cgatcggatg accggaatcg
      301 gaggtttccc gtatcgccat atgcaacggc agcgcaccga ggaacggcac gccaagccgc
      361 tcggcctcgt gacgcgcccc accatgacta aaaatgtcgg agcgatgccc gcattttggg
      421 cacagaaagt agctcatatt ctcgatgatg cctagaaccg gcacattcac gcgcttgaac
      481 atattaagtc ccttgcgcgc atctatgagc gcgaggtcct gcggcgtcga cacgatgacc
      541 gcgccagaaa gaggcacttg ctgagccatg gtgagctgag cgtcgccggt gccgggtggc
      601 atatcaacga caagcatatc caactcgccc cacgcgacat ccttcagcat ttgtgtcaat
      661 gccgagataa ccatcggccc acgccaaatc atcggcgtct cttcctccac gagaaagccg
      721 atggacatca ccttcaaacc atacttctcg ataggagcga ggagattatc gccgatgagt
      781 tcgggcctgc tctttatggc gagaagccgc ggtactgagg ggccgtaaat atccgaatcc
      841 aagactccaa cgcggagccc attttcccgg aacgcgagcg ccaagttgac tgccgtggtc
      901 gatttcccca caccaccctt tcccgaggcg actgcaatga tgtgcttgat accaccgatg
      961 ccttgaccag aggcaccttt ctctggaact tctttcgtgt ccgcggtcag cgtgaccatt
     1021 gcgcccgcca cccccggaag agctttcacc acggtttctg ctgactttcg gacaggctca
     1081 taagccttcg cgtcccgttg gggaaccgta agcgcgaagt aaaccttacc cgcgtttata
     1141 acgatctctg aaagaatgcc caaggtcacg atatcctgac cgttcggtgc cttgacgctt
     1201 tttagcgcct tcaaaacatc gtctttcgtg agagtttctg tcatcgaatc cgccggtgag
     1261 aggggtgaaa aagcgagtga tctctatcta atgggtggcg cgcgattgcg aaagtcacct
     1321 gatcgagatt cgtagttcat gagccgcccg tggttgggct accccaacag aaacttcgag
     1381 agtttgcgat cgcccccgat atggacagcc tagcggtctg gtcaaggggc gcgatgatct
     1441 cctcctggcc gttgacattc gtgttgtcgc tgaagaaccg ctcacgcttt ttctgaaccg
     1501 ccaggagatt gtcaccaatg acgattggcg attactccga ccttcttgcc gcaggaatga
     1561 tcaagtcgag tggcgaaatg cgacgcgagc ttgatactgt agtcgtgcgc aacggcctgc
     1621 acgaccaatt tcgaggcgaa acccaagaag aggtgcccac ctcggggctg tgcgaacggc
     1681 acagttttcc gagatcctat ggacgaaatt gaaggcgtcc aactcagtaa ggaggcgcgc
     1741 cttataatag aacgttcggc tctatgccct cagaaaaaga tcaatactgc gccaagcctt
     1801 tatctcaagg cgggagcgat caaggctgcg tattctgcaa agaagatcgc ccgccaatct
     1861 ctgtggagga cggtggcagg cacaatgccg tcgataaaat agccctgtat ggcgctgaat
     1921 aatattgcgg cccatgacaa gctcatttac acgattggcc gccttgcgag cgaaattgtc
     1981 attaagaccg tgaagatagg tctcctgatc ctggcctctc gctcaagttt tacggcctgg
     2041 ggcgttgaac ttgctcgccg ctccggcttg accttgatcg gtcgcatgct gggaaagcgc
     2101 tttgttgccc tttccggcga ggtgcccatc ttatatgacc acgacgtaat gccgttaagg
     2161 atgaggatgc acggcatcga ggcaagggcg ccagggatga aggctcataa gtggaacagg
     2221 tagcaggcgt tatccttgcg ggcggtcttt cccgccggat gggaggcggc gacaaatccc
     2281 tgcgcaccct caatggcaag ccattaattc tccgcgcgat cgaacggctt ggcgggcaag
     2341 ctggcccaat cgccctcaac gccaatggcg acgcttcccg cttttctgct tttgggcttc
     2401 ctgtgatccc ggacgcgacg gcggatttcg ccggaccgct cgctggcgtt ctggccggac
     2461 tgcgctgggc ggcagaagca gcgccggatg cgcgctttat cgtaaccgcg gcttgcgaca
     2521 cacctttctt cccttcggat ctcgtggaaa aactgctggc ctccacaggg ccgacctacc
     2581 cctgcatcgc gctcgcggct tcgggcgaaa acgctcatcc ggtcttcgcg ctctggcccg
     2641 tagctcttg
//
LOCUS       DATMGX010000677         3660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_240242, whole genome shotgun sequence.
ACCESSION   DATMGX010000677 DATMGX010000000
VERSION     DATMGX010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240242"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VNR65_11115"
     CDS             complement(<1..180)
                     /locus_tag="VNR65_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19265.1"
                     /translation="MATLGEGGLLATAYVAEKVQTLLMHGVGFCFGLSSWLGVPDNPD
                     HIRADDSHSPLHLINC"
     gene            complement(232..720)
                     /locus_tag="VNR65_11120"
     CDS             complement(232..720)
                     /locus_tag="VNR65_11120"
                     /inference="COORDINATES: protein motif:HMM:NF021165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HWJ19266.1"
                     /translation="MKTRLTGLSFAASVAMASACWAPLALADRPTIIPDSTVTKECGS
                     CHMAFPAEMLPARSWTAIMEGLSSHFGENAALDAAKGKEILDWLKAHAGDARNSNGHY
                     LRGLANGETPLRITETPYWVREHRKEVKESDYANPKVKSKANCVACHKDAAKGTFEGD
                     DD"
     gene            complement(745..1161)
                     /locus_tag="VNR65_11125"
     CDS             complement(745..1161)
                     /locus_tag="VNR65_11125"
                     /inference="COORDINATES: protein motif:HMM:NF020652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HWJ19267.1"
                     /translation="MKKTVIAFVCLLGSAPLAAEAGPAQTAILEKYTAEAKAADPAFS
                     GFSGERGKSLFFSEPAAGKPDTPSCTSCHTKNPKEPGQTRAGKEIGPMALSVKSDRYS
                     DSQETEKWFGRNCDSVLGRPCTPIEKGDFITFMASQ"
     gene            complement(1158..2303)
                     /locus_tag="VNR65_11130"
     CDS             complement(1158..2303)
                     /locus_tag="VNR65_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWJ19268.1"
                     /translation="MTATDFRQTAGDADRTIIVWDWGVRVFHWGLVLCVGVCICTGFL
                     AAPNWLNLHLAAGTVIAGLIVFRLVWGWTGSGYARFSSFAVSAPRLRQHFSDIMKRRR
                     VRHLGHNQAGALMVYALLAVLILLVISGAIALGGAVKQGPLAFALSFAAGRTAREIHE
                     LLAFGLLAMIAAHWAGVAFESWRLRENLVRAMFTGRKSDCSASSEAPAPARPAEAATI
                     VLGIAAIAVLGVIALSQIPAKGVPTAPLHPAYVKECGDCHFAYPPSLAPAATWKAVMD
                     GMADHFGENATLDPTKASSIRDWLTANASERWDTRAANMLRRVNVADPQRITSTPGWT
                     RVHEDIPDSVFKSKAVGAKGACKACHSDAETARFDPQSIAIPKEAQP"
     gene            complement(2891..3592)
                     /locus_tag="VNR65_11135"
     CDS             complement(2891..3592)
                     /locus_tag="VNR65_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HWJ19269.1"
                     /translation="MSRLHTHTENHLVERIGWLRAAVLGANDGIIATASLIVGVAAAA
                     ATQHDVLIAGVAGLVAGAMSMAAGEYVSVSSQADTERADLAREAAELSGNPEFELQEL
                     SEIYVKRGVDQALARQVAQQLMAKDALTAHARDELGISEITTARPVQAALTSAAMFTV
                     GAAMPLLMVVLSPANLLVPVVSAASLGFLALLGAMGAKAGGANILRATARVTFWGALA
                     LALTAGIGKLFGTVV"
BASE COUNT          683 a         1122 c         1104 g          751 t
ORIGIN      
        1 gcagttgatg aggtggagcg gcgaatgaga atcgtccgcc cggatatggt ccggattatc
       61 gggcacgccg agccaactcg aaaggccgaa gcaaaagccc acgccgtgca tcagtaatgt
      121 ctggactttc tctgccacgt aagcggtggc gaggagacct ccctccccga gggttgccaa
      181 cctcggagag ccagaggtaa tcgctttctt gtcgccagca ccgccgaaag atcaatcgtc
      241 gtcaccctcg aaggtgccct ttgctgcatc cttatggcac gccacgcaat tcgccttgct
      301 cttgaccttg ggattggcgt agtcggactc cttcacctct ttcctgtgct cgcggaccca
      361 gtaaggcgtt tccgtgatcc gcaagggcgt ttcgccgttt gcgaggccgc gcagataatg
      421 tccgttggag ttgcgcgcgt cgcccgcgtg cgccttcagc caatcgagga tttccttccc
      481 cttggcggcg tcgagtgcgg cattctcgcc aaagtgactg gataggccct ccatgatagc
      541 cgtccatgac ctcgcaggaa gcatttccgc cgggaaggcc atatggcatg agccgcattc
      601 tttcgtcacc gtggagtcgg gtatgatcgt gggcctgtct gcaagggcca gaggcgccca
      661 gcatgcgctt gccatcgcca cgcttgccgc aaacgatagt ccggtcagtc tcgttttcat
      721 tttcaatccc tctcccttga gccgctactg gctcgccatg aatgtaatga agtcgccctt
      781 ctcgatgggc gtgcagggcc gtccgagcac gctgtcgcaa ttgcgtccga accatttctc
      841 ggtctcctgg ctatcgctgt agcggtcgga tttcacgctc agcgccatcg ggccgatttc
      901 cttgccggcg cgcgtttggc ccggctcttt cgggtttttc gtgtggcacg atgtgcagga
      961 gggggtatcc ggcttgccgg ctgccggttc ggaaaagaac agcgacttcc ctcgctcccc
     1021 tgaaaagccg gagaaggctg ggtcggcggc cttggcctcg gcggtgtatt tctcaagaat
     1081 ggccgtctgt gcaggtcctg cctcggccgc caacggcgcg gaaccgagca gacagacaaa
     1141 ggcgattacg gttttcttca tggctgtgct tccttcggaa tggcgatact ttgtggatcg
     1201 aagcgcgctg tttccgcgtc gctgtggcag gccttgcaag cgcccttcgc gccaacggct
     1261 ttcgatttga aaacggagtc tggaatatcc tcgtggaccc gcgtccagcc cggcgttgag
     1321 gtgatccttt gcggatcggc tacgttcacg cggcggagca tgttggcggc tcgcgtatcc
     1381 cagcgctcgc tggcattggc ggtcagccag tcccgaatgc tcgaagcttt cgtgggatcg
     1441 agagtggcat tttctccaaa atggtccgcc atcccatcca tgaccgcctt ccaagtggcg
     1501 gcaggagcga ggctcggcgg gtaggcaaag tggcagtcac cgcattcctt gacgtatgcc
     1561 ggatggagcg gcgcggtcgg aacgcctttt gccgggatct gcgataaggc gatcacgccc
     1621 agaaccgcga ttgccgcgat gccgagcacg atcgtggcag cctccgcggg gcgggcaggt
     1681 gccggtgcct cagaactcgc tgagcagtca ctcttgcgcc ccgtgaacat ggcgcgcacc
     1741 agattctcgc gcagccgcca gctttcgaag gcgacacccg cccaatgggc ggcgatcatg
     1801 gcgaggagac cgaaagccag aagttcatga atttcgcggg ctgttcttcc tgcagcgaag
     1861 gaaagcgcga aagcgagtgg tccctgcttc acggctccgc caagcgcgat cgcgccggaa
     1921 atgacgagaa ggatgagaac cgccagcagc gcataaacca tcagcgctcc ggcctgattg
     1981 tggccgaggt gacggacgcg ccgccttttc atgatgtcgc tgaagtgctg ccgtagcctg
     2041 ggggccgaga ccgcgaagga actgaaacgc gcgtaccctg atccggtcca gccccaaacc
     2101 agccggaaaa cgataagccc ggcgataact gtgcccgccg caaggtgaag gttcagccag
     2161 ttcggcgcag caaggaagcc tgtgcaaatg caaacgccta cgcagagaac aaggccccaa
     2221 tgaaagaccc gcacgcccca atcccatacg atgatagtgc ggtctgcgtc tccggctgtt
     2281 tgcctgaagt ctgttgcggt cacgttgcca ccgttggttc gatacgtgac cgctttttat
     2341 ccaagtggag ctgtcgagtt cctgtcaggc atttacagcg gggctttagg aagctctccg
     2401 ttgatcaact ctgtctccat tcccgttcgt tagcggcgtt ctcaggccac tgcggtaggt
     2461 ttttgactgg tggcgtaggc ccacggcatc cgctcatcga tgcggcgcat gggtgttttt
     2521 gaacaaggca agaaacagtc aggcagtcgg cactccggca gcaatatctt gagtttcagc
     2581 gcaatgacag ccgccttcgc cgcaggcttt cttgcatcat cgctctgcta cctggccatt
     2641 ttgacagtac gggcttgagt tggtgcctct ctcgaccacc ttgccgccgc ccgttatgtc
     2701 cccaagcgat gcttctcgcg acgctaccgg actgcggtca acaacgtcag gatcggaaac
     2761 tacttacgtc atcgggaaat gatgaaccag gtgatcttcg gcccgctttt ggggcgggaa
     2821 tagtggtcgc atacacgagg cctgggcgcc aatcatgcgc catactgcat cccgcctgcg
     2881 gtcccgactt tcagacaacc gtgccgaaaa gcttcccaat accggcggtt agagctaaag
     2941 ccaacgcgcc ccaaaacgtg acccgggccg tggcgcgcag aatgttggcg cctccagcct
     3001 ttgcgcccat cgcccccaaa agggcgagaa agcccaacga cgcggcggac acgacgggaa
     3061 ccagaagatt ggcgggagat aggacgacca tcagcaaggg catcgccgcg ccgaccgtaa
     3121 acatggcggc cgaagtcagc gcggcttgga caggccgcgc ggtggtgatt tccgaaattc
     3181 caagctcatc gcgcgcatgg gcggtcaagg catccttcgc catcagttgc tgagcgacct
     3241 gcctggcaag cgcctggtcg acgccgcgct tgacgtagat ctctgaaagc tcttggagtt
     3301 cgaactctgg gttcccgctt aattcagcgg cttcgcgcgc cagatcggcc cgctcagtgt
     3361 ccgcctgcga actgacggat acatactcgc cagcggccat cgacatggct cccgccacca
     3421 gtcccgcgac gcctgcgatc aagacgtcgt gctgggtcgc ggccgccgcg gcgacgccga
     3481 cgatcaagct tgcggtcgcg atgatgccat cgttggctcc gagcacggct gcccttaacc
     3541 aaccgatgcg ctcgacgagg tgattttctg tgtgcgtgtg aagacggctc atcttgttcc
     3601 tcggatcggt ttcgcctgcg gccgcgcgta gaactgttgt gggatctcaa cagcgatgag
//
LOCUS       DATMGX010000678         5865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_448270, whole genome shotgun sequence.
ACCESSION   DATMGX010000678 DATMGX010000000
VERSION     DATMGX010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448270"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(779..1552)
                     /locus_tag="VNR65_11140"
     CDS             complement(779..1552)
                     /locus_tag="VNR65_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWJ19270.1"
                     /translation="MEGHHVQVALFVTCLVDVFRPSAAFSVVKLLEEAGCEVAVPATQ
                     TCCGQPAYNSGDRETAREIAKQVIAAFETFPYVIVPSGSCAGMLSKHYPQLFDPETEP
                     SWHARATGFASRVRELTAFLADDLGVDFTGRPYPRRVTYHDSCSSIREMGVASQPRKL
                     LKSLDGIELVEMTDRDVCCGFGGAFSVKYPEISNAMATQKLDRVLETKADVLTGPDLG
                     CLMNIAGKAAREGKAIEVRHIAEILAGDVDEPGLCQPGS"
     gene            complement(1601..2569)
                     /locus_tag="VNR65_11145"
     CDS             complement(1601..2569)
                     /locus_tag="VNR65_11145"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ19271.1"
                     /translation="MTSIADLEIFARVVTAGSMSAAGRELDLSPAVVSKRISHLETRL
                     GTRLFHRTTRQLQLTETGRGFYERVVQILATVQEAEAFVSSGHQRAGGSLKITAPTGF
                     SRMHIAPYLGKFQKQYPDLGIEIIATDNILDIVREGIDVAIRVSELDDSSMVAKKLAP
                     CRRLFCAAPDYLKEHGTPKTLADLSKHKILTENNTAWRLQGPDGITSLRLSGDIKTNS
                     SDVVHEALLAGCGIALRSTWQVRDDLLSNKLVQILPQYSEAPGVAVYAIYPDKQYIPA
                     RLRVFVDFLADIYGPAPYWDEGLDLDGASRLAPKKTLLKAEEPAGV"
     gene            2929..4233
                     /locus_tag="VNR65_11150"
     CDS             2929..4233
                     /locus_tag="VNR65_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWJ19272.1"
                     /translation="MPLAVVLPRTTAEVCRILKFCHENTVRIVGRGAGTSLSGGALPT
                     GDAVVIGLSRMNRVLDVNFLDRTATVEAGITNLGISNAVAHARFFYAPDPSSQLACTI
                     GGNIAMNSGGAHCLKYGVTTNNVLKVKMVLMNGEIVELGGDYLDPQGYDLLGLVIGSE
                     GQFGIVTEVTVRILRQPEGARPILIGFPSTESAGGCVAAIIADGLIPVAIEFMDKPAI
                     HVCESFAKAGYPMDAEAMLIVEVEGSESEIEDQIGRITEIASRFDPTTLRVSQSEEEA
                     AAIWKGRKSAFGAFGRISDYYCMDGVIPLGKLAAVLKQVQDVGEKHNLKVANIFHAGD
                     GNLHPLILYNANDEREKATAEQCGADILKLCVDAGGCLTGEHGVGIEKRDLMRYQFSE
                     ADLLQQMRVKAAFDPQWLLNFGKVFPLDLQTRFAAEAAKNHG"
     gene            4226..5449
                     /locus_tag="VNR65_11155"
     CDS             4226..5449
                     /locus_tag="VNR65_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWJ19273.1"
                     /translation="MADIFMPGDEAELAGFVEAAKAGNYPLEVCGFRSKREAGRPVNP
                     AAVVSTAKLSGITFYEPGELVISAKAGTPLHEVEAALARKNQELPFEPADFSRLYGPD
                     PMAASLGSVAAMNISGPRRILRGAARDYLLGIRAVNGEGVAIKSGGRVMKNVTGVDLV
                     KGLCGSWGTLAVLTELTFKVLPKAPDTSTLVFLGLPDESGVGVLSEAMGTPYEVSGTI
                     HLHRSLVSRLSDPEIASANMALTALRLEGDPQSVAYRMEKLRRALSPFGETYVLDRQK
                     SRSFWADIRSLAFLSANFERPLWRITVAPSKAAQIVRALSAFIDLSAAYEWSGGLIWL
                     ETPQSSEASVTELRRVLAEFEADSMLMRAPRSVRSSIEVFHPLPLTKMKLVQAVKKAF
                     DPVGVLNPGRMYAGV"
     gene            5455..>5865
                     /locus_tag="VNR65_11160"
     CDS             5455..>5865
                     /locus_tag="VNR65_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWJ19274.1"
                     /translation="METHFTLEQLRDPRLKEADDILRSCVHCGFCIATCPTYLLLGDE
                     RDSPRGRIYLIKSMLEGKAAPRQVRPHLDRCLSCYSCMTTCPSGVDYMHLSDYARQLI
                     EKSSMRAPNDESRRKLLRNVLPHPKRFRFALLALF"
BASE COUNT         1235 a         1697 c         1688 g         1245 t
ORIGIN      
        1 gccgcctgcg cggcacctgt tccggccgcg cgcgcagtta attcgcgaag ccgcaacata
       61 tttaacggac ttttgcttgt cccagttaat gagaaaattc cggcattaaa ttctatttaa
      121 tgggcggtca caggcgaagg atagtcgttt gttctcaatg gtctatcgga tttacttccg
      181 gccgggaaac aggctcggcg ggaagtaatc gcggcaggaa taaattgctt gttaatcatc
      241 ttggtaaccg gaaataattc cggcgcgctc ctgttaaccg tggccggtta atccatccgc
      301 gagcgaagga agcgtttacc aaaacgcgtg ctgcgagcgc cgtatgcttc gatgcattcc
      361 gacgcggttg attgcgccgc aaccggcttc gtttagcgca cgccgcgtga gccactacaa
      421 cgcggaaaat tattctcgag cgggacagtt ttgctgcgcc gcacaaacga aatcatttgc
      481 acaagtgaat gaaacgcacg atcatttgac tagtccgcag agaaacgcag ccaagtcgaa
      541 ggatacgcta cgatgtctgt tgcgagtgag ctaagcatca tcacaaccct gatggtcttc
      601 ggatttctgg caggagttat tcttggtgtg ctctaagcca gtctcctatc cggtcttgat
      661 taccgggatg ttcctgcccg cagaagaagc cttgaaaacc ttggcgaatt ttcgctaacc
      721 tgtcatggcc ggcttgaccc ggccatgaac gacgggattg gcggagacat cccggtaatc
      781 aagatcccgg ttgacacaac ccgggctcat cgacatcacc cgcgaggatc tcggcgatgt
      841 gacgaacctc gatcgctttg ccctcgcggg ccgcttttcc cgcaatgttc atcaggcagc
      901 cgagatcggg tccagtcagc acatcggcct tggtctcaag gacgcggtcc agcttctggg
      961 ttgccatcgc atttgaaatt tcggggtatt tgaccgaaaa cgcgccgccg aagccgcagc
     1021 acacatcgcg gtcggtcatt tcgacgagtt cgatcccatc caggcttttc agaagttttc
     1081 gcggctgcga ggcgacgccc atctcccgga tggatgagca ggaatcgtga taggtgacgc
     1141 ggcgcgggta ggggcgtccg gtaaagtcga cgcccaaatc atcggcaaga aacgccgtca
     1201 actcccgcac acggctcgcg aagcccgtag ccctggcgtg ccaggacggc tctgtctccg
     1261 gatcgaagag ttgagggtag tgcttgctca gcattccggc gcaagagccg gacggcacga
     1321 tcacataggg gaaagtctcg aatgcggcga tgacctgctt ggcgatctct cttgcggtct
     1381 cgcggtctcc ggaattatac gcgggttgcc cgcaacaggt ctgcgtggca ggcacagcga
     1441 cttcgcatcc agcttcttca agcaacttca caacagagaa ggctgccgat ggccggaata
     1501 catcaacgag acaggtgacg aagagagcaa cctgaacgtg atgaccttcc atggatgcta
     1561 tccttcaggc tctcgctggg catgctgcag aaggtcgatg tcaaactcct gccggttcct
     1621 cagccttcaa gagagttttt tttggagcca gacgcgatgc gccgtccaga tcaagaccct
     1681 cgtcccagta tggagcaggg ccgtagatat ccgcaaggaa gtcgacgaac acgcggagcc
     1741 tcgccgggat atattgctta tccgggtaga ttgcatagac ggcgacgccg ggggcctcac
     1801 tatactgcgg aagaatttgt acaagcttat tggacagaag gtcatccctg acctgccatg
     1861 tcgagcgtag cgctattccg cagcccgcca agagcgcctc atgaacgacg tcgctgctgt
     1921 ttgtcttgat atcccccgag agcctgagcg aggttatgcc gtcagggcct tgaagacgcc
     1981 aggccgtgtt attctccgtg aggattttat gcttggagag gtccgcaagc gtttttggcg
     2041 ttccgtgctc cttcagatag tccggcgctg cgcaaaacag acggcggcaa ggcgcaagtt
     2101 tcttcgcaac catgcttgaa tcgtctaatt ccgatacgcg gattgcaacg tctattccct
     2161 cgcggacgat atcgaggata ttatcggtcg caataatctc gatgcctagg tcgggatatt
     2221 gtttctggaa cttcccaaga tagggagcga tatgcatacg gctgaaaccg gttggcgccg
     2281 tgatcttgag cgatccgccc gcgcgctgat ggccggagga tacgaatgcc tcagcctcct
     2341 gcaccgtcgc caggatttgc accactcgtt cataaaagcc gcggcctgtt tcggtaagct
     2401 gaagttggcg cgtcgtgcga tgaaacaacc ttgtgccgag ccttgtttca agatggctga
     2461 tacgcttgct gactacggct ggcgaaaggt ccaactcgcg tccagcggcg gacatactgc
     2521 ccgcggtgac cacccttgca aaaatctcca gatctgcaat cgaagtcacg agcccctcct
     2581 aaccccagcg gaaattgtat acaatccaat gcgatagata cgcaaagctg cttataagaa
     2641 ctcaccccgc ccgcatgtca agcatttctc tagctacata actaaagata atgttcctgc
     2701 atttatataa tgcacccttt gcacccacaa catgttgtgg gtagagtcat gagaagatag
     2761 ggaacaccta tgtcgccaat ccaaatgccc aaagccgacg cctcagtcct tggccgccgc
     2821 gaacaaatct taagcgggct ctcccgtgcg cttcgatcga attctgtcat tagcgatgaa
     2881 gccggccttc gcgccttcga aacagatgcc ctcacagctt atcattctat gccgcttgcc
     2941 gttgtattgc caagaacaac ggcagaagta tgccgaattc tcaagttttg ccatgaaaat
     3001 accgtaagga ttgttgggcg gggcgcagga acatctctct ctggcggtgc tctgcccaca
     3061 ggcgacgcag tcgtcatcgg cctttcgcgc atgaatcggg ttctcgacgt gaatttcctt
     3121 gaccggacgg caaccgtcga ggctggcata acgaatctcg gtatctcgaa cgcggttgct
     3181 catgcacggt ttttttatgc tcccgacccg tcgagccagc ttgcgtgcac gatcggcggt
     3241 aatatcgcta tgaattcagg cggcgcgcat tgcctgaagt atggagtgac aaccaataac
     3301 gtcctgaaag tcaagatggt tttgatgaac ggcgagatcg ttgaacttgg cggcgactac
     3361 ctcgatccgc agggctacga tctgctaggg cttgtcatcg gctccgaagg ccagttcggc
     3421 atcgtgacag aggtgaccgt gcgcatcctg cggcaaccgg aaggggcgcg cccgatcctc
     3481 atcggcttcc cgtccaccga atcagcaggg ggttgcgttg cagcgatcat cgccgatggt
     3541 ctcatccccg tggcgatcga gttcatggat aagcccgcca tccacgtttg cgagagcttc
     3601 gcgaaggccg gatatccgat ggacgccgag gcgatgctga tcgtcgaagt ggagggctcg
     3661 gaaagcgaga tcgaggatca gatcggccga atcactgaga ttgccagccg attcgatccg
     3721 acgacgctgc gcgtaagcca gagcgaagag gaggccgccg cgatctggaa gggccgcaag
     3781 tccgcgttcg gcgcgtttgg gcgcatttcg gattactact gcatggatgg cgtgatcccg
     3841 cttggcaagc tggcggcagt gctcaaacag gttcaggacg ttggagagaa gcacaacctc
     3901 aaggtcgcaa acatcttcca tgcaggggat gggaatttgc acccgctgat cctttacaac
     3961 gccaacgacg agcgcgaaaa agcgaccgcc gagcaatgcg gcgcagacat tctcaagctg
     4021 tgcgtggatg cgggcggctg ccttacgggc gaacatggcg ttggaatcga gaaacgggat
     4081 ctcatgcgct atcaattttc ggaagcggac ctgctccagc agatgcgcgt gaaggcggcc
     4141 ttcgatccgc aatggctttt gaacttcggg aaagtgtttc cgctggacct ccaaacgcgt
     4201 ttcgccgcgg aggccgcgaa aaatcatggc tgacattttc atgccaggcg atgaggcgga
     4261 gcttgccggc ttcgtcgagg ccgcgaaggc cggcaattat ccgctcgagg tctgcggctt
     4321 tcgctcgaag cgcgaggcgg ggcgccccgt caatcctgcg gcggttgtgt ccacggcaaa
     4381 gctgtccggc atcacgttct acgaacccgg cgaacttgtg atttccgcca aggccgggac
     4441 gccgcttcac gaggtcgaag ccgcgcttgc aagaaaaaac caggagcttc cattcgaacc
     4501 tgcggacttc agccgccttt atggacccga cccaatggct gcgagcctcg gctcggtcgc
     4561 ggccatgaat atttccggcc cgaggcgcat cctgcgcggg gccgcgcgcg attatctcct
     4621 gggcattcgg gcagttaacg gcgagggcgt ggccataaag tcaggcggcc gcgtgatgaa
     4681 gaatgtgacg ggcgtcgatt tggttaaagg tttgtgcggc tcctggggca cgctcgctgt
     4741 attgaccgaa ctcactttca aagtgttgcc caaggcccct gacacttcaa cactcgtctt
     4801 tctcggcctt cccgatgagt cgggcgtcgg tgtcctaagc gaagccatgg gaacgcccta
     4861 cgaggtgtcc ggcacgattc acttgcaccg ttcgctcgtc agcaggttga gcgatccgga
     4921 aatcgcttca gcgaacatgg ccttgacggc ccttcggctg gagggcgacc ctcaaagcgt
     4981 cgcctatagg atggagaagc tccggcgcgc actgtcgccc ttcggcgaga cctacgtgct
     5041 cgatcgccag aaatcccgca gcttctgggc cgacatccgc tcgctcgctt ttctgtccgc
     5101 gaacttcgag cggcccttat ggcggattac cgtcgcgccg tcgaaggccg cgcagatcgt
     5161 gcgcgctctc tccgccttta tcgacctcag cgccgcctat gagtggtccg gcggtctcat
     5221 ctggctggaa acaccgcaat cgtcggaggc aagcgtcacg gaattgcgcc gggtgctcgc
     5281 ggaattcgag gcggattcga tgctcatgcg cgcgccacgg tccgtgcgct ccagcatcga
     5341 ggtctttcat ccgctgccgc tcaccaaaat gaagctggtt caggcggtca agaaagcctt
     5401 cgatccggtt ggcgtgctca atcccgggcg catgtatgcg ggggtctgaa aaccatggaa
     5461 acgcacttca cgctcgaaca actccgcgat cctcgcctca aagaagccga cgacatattg
     5521 agaagctgcg tgcattgcgg cttctgcatc gccacctgcc cgacctacct tctgctcgga
     5581 gacgagcgcg acagcccaag gggccggatc tatctcatca agtccatgct tgaggggaag
     5641 gcggcgccga ggcaggtccg cccccatctc gatcgctgcc tgtcctgcta ctcctgcatg
     5701 acgacatgtc cgagcggcgt cgattacatg catctgtcgg actatgcgcg ccagctgatc
     5761 gagaaatcga gcatgcgcgc gccgaacgat gagtcgcggc gcaagcttct ccgcaacgtt
     5821 ctgccgcacc cgaagcgatt ccggtttgcg cttctcgccc tgttc
//
LOCUS       DATMGX010000679         3584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_224303, whole genome shotgun sequence.
ACCESSION   DATMGX010000679 DATMGX010000000
VERSION     DATMGX010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224303"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VNR65_11165"
     CDS             <1..1193
                     /locus_tag="VNR65_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWJ19275.1"
                     /translation="PIRLGWFAIAFPALILNYLGQGALMLGHPPAHDGELFYNMVPRE
                     LLIPMVVLATLATVIASQGLISGSFSLASQAVALGLFPRLRIVHTHDAHEGQIYIPFV
                     NWALFAGCAALVLGFRSTESLAALYGLAVSGVMVITSAAMIPVGTRYWGWSPLASSAL
                     FGFFTLVNAVFCVASSLKLLEGGYIPLGIGLAVFAIMLTWRWGRRATFAAYTAKHTMT
                     MRRLVEFHKREPAYMERIGLLMTPKHLSSLNDNAPALLQLLYDRYGILPRHLIFVQVA
                     HRKIPYIHDGRYGITVFHKDDSSSIIAVTLQFGFMEEPNVEAALEDMARHREIDLPID
                     QHRWIVHVSIEHLLPSRNMRAWGRARLRLFRILRRISQPAHYFYGLGDNVQLSAEIIP
                     VRLK"
     gene            1345..3498
                     /locus_tag="VNR65_11170"
     CDS             1345..3498
                     /locus_tag="VNR65_11170"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HWJ19276.1"
                     /translation="MNQLLWIIIACGALSIVYGALTTRSLLAASAGNARMQEIAAAIQ
                     EGASAYLSRQYTTIAIVGVVIFGVLAYLLSFTVAIGFLIGALLSGAAGFIGMLVSVRA
                     NVRTTQASSESLAKGLSMAFKAGAVTGLLVAGLALLGVTIYYGILTGYLGYAPNDRRV
                     VDALVGLGFGASLISIFARLGGGIFTKGADVGGDLVGKVEAGIPEDDPRNPATIADNV
                     GDNVGDCAGMAADLFETYAVTVVATMVLSSIYFAYDPALLARMMIFPLAIGGICVVTS
                     IIGTFFVRLGSSNSIMGALYKGFIATGILSLIALYPLTSYIVGLDTTLKANGTSFTGL
                     DLFYCGIVGLVVTTLIVVITEYYTGTGYRPVKSIAQASVTGHGTNVIQGLAVSLESTA
                     LPALVIMAGILITFNLAGLYGIAIAVTTMLALAGMVVALDAFGPVTDNAGGIAEMADL
                     PSSVRKTTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYTEDLRYFTAQAASDTTH
                     FQYFRGLNVDFSLSNPYVVVGLLFGGLLPYLFAAMGMTAVGRAASSIVEEVRRQFREK
                     PGIMQGTEKPDYGRAVDMLTKAAIKEMVIPSLLPVLSPIVIFVLITFIGGKAAGFAAL
                     GAMLLGVIVTGLFVAISMTSGGGAWDNAKKSFEDGFVDKDGRRHMKGSEAHKASVTGD
                     TVGDPYKDTAGPAVNPMIKITNIVALLILAVLAHQ"
BASE COUNT          632 a         1097 c         1032 g          823 t
ORIGIN      
        1 gacccattcg gctgggctgg ttcgccatcg catttccggc gctcatcctg aattacctcg
       61 gtcagggcgc gcttatgctt gggcatcccc cggcccacga tggcgagctt ttctacaata
      121 tggtgccgcg cgagttactc attcccatgg tggtgttggc cacgctcgca acggttatcg
      181 cctcgcaggg gcttatttca gggtcgttct cgctggcctc gcaggcggtc gctctcggcc
      241 tgttcccgcg ccttcggatc gtccataccc acgacgcgca tgaaggccaa atttatatcc
      301 cctttgtgaa ctgggccttg ttcgccggtt gcgcggccct ggtgctcggt ttccgctcta
      361 cagaatcgct ggccgcgctt tatggccttg ccgtctcggg cgtgatggtc atcacatccg
      421 ccgccatgat tccagtgggg actcgttatt ggggatggag cccgctcgca agcagcgctt
      481 tattcggctt cttcacgctc gtgaatgccg tgttctgcgt ggcgagttcg ttgaagctcc
      541 tggagggcgg atatatcccc ttgggcatcg gcctcgcagt cttcgccatc atgctcacct
      601 ggcgttgggg acggagggca acgttcgcag cctacaccgc caaacacacc atgacgatgc
      661 gcaggctggt cgagttccat aagagagaac ccgcctacat ggagcgcatt ggccttctga
      721 tgacgccgaa acatctgtcg tcgctcaatg acaatgcgcc cgcattgctc cagcttctct
      781 atgaccgcta cggtatattg ccgcgccatc tgatcttcgt gcaggttgcg catcgcaaga
      841 ttccctacat ccatgatggc cgttatggca tcacggtttt ccataaggac gactcttcca
      901 gcatcatagc cgtcaccttg caattcggct tcatggagga gccgaacgtg gaagcggcgc
      961 ttgaagacat ggccaggcat cgcgaaatcg acctgccaat cgaccagcac cgctggatcg
     1021 tgcacgtatc gattgaacac ctgctaccgt ctaggaacat gcgcgcgtgg ggccgagcgc
     1081 gtctgaggct cttccgcata ctgcgccgca tatcgcagcc cgctcattat ttctatgggc
     1141 taggcgacaa cgtgcaattg tcggccgaga ttatacccgt ccgtttaaag tagttagtaa
     1201 cctttctcgc gaacctgcgc cctttagcat attgcattgc aatagcaagt tttctaaggt
     1261 cgggcggctt aagccattcc cgttcttatc ccggtgggcg gcaaccaagc ccagaatcac
     1321 acgtttcagg accacgaaaa acccatgaac caattacttt ggatcatcat agcgtgtggc
     1381 gcgctctcca tcgtgtatgg ggcgctcacc acacgctcgc tgctggcggc aagcgccggc
     1441 aatgcgcgca tgcaagaaat tgcagccgcc atccaggaag gcgcgtcagc ctatctctcg
     1501 cgccaatata caaccatcgc catcgtaggc gtggtcattt ttggcgtact cgcctatctt
     1561 ctctcattca cagtggcgat cgggtttctg atcggcgctc tcctgtccgg cgcggcgggc
     1621 tttatcggca tgctggtctc ggtgcgggcg aatgtgcgca cgacccaggc ctcgtcggag
     1681 agcctggcaa aagggctctc catggcgttc aaggccggcg cggtgacggg gcttcttgtt
     1741 gcaggcctcg cccttcttgg ggtcaccatt tattacggta ttttaaccgg gtatctcgga
     1801 tacgcgccca atgacaggcg cgtcgtcgat gccctcgtcg gtctcggttt cggcgcatcg
     1861 ctgatctcga tctttgcgcg tcttggcggt ggcatcttca ccaagggcgc tgacgtgggc
     1921 ggcgacctcg tcggcaaggt tgaagccggt atcccggagg acgatccgcg caacccggcc
     1981 acgatcgcag acaacgtcgg cgacaacgtg ggcgactgcg caggcatggc ggctgacctt
     2041 ttcgaaacct atgctgtgac ggttgtggcg acgatggtgc tgtcatccat ctatttcgcc
     2101 tatgatcccg cgcttctcgc gcgcatgatg atcttccctc tggccatcgg cggcatttgt
     2161 gtcgtgacct cgatcattgg cacattcttc gttcgcctcg ggtcgagcaa ctcgatcatg
     2221 ggcgcgcttt acaagggctt tatcgcaacg ggcatcctgt cgctcatcgc gctctatccg
     2281 ctgacgagct acatcgtcgg cctcgacacg acgctcaagg cgaacggcac ctccttcaca
     2341 ggcttggatc tgttctactg tgggatcgtc ggccttgttg tcaccaccct gatcgtggtc
     2401 atcaccgagt actatacggg cacgggctac cggccggtaa agtccattgc gcaggcttcg
     2461 gtgacgggac acggcacgaa cgtgatccag ggccttgcgg tctcgcttga gtcgacggcg
     2521 cttcccgctc tcgtcatcat ggccggcatc ctcatcacct tcaacctcgc tggcctttac
     2581 ggcatcgcga ttgcggtcac gacgatgctt gcgcttgcgg gcatggtggt tgcgctcgac
     2641 gctttcggcc cagtgaccga caacgcgggc ggcattgcgg aaatggccga ccttccttcc
     2701 tcggtccgca agaccacgga cgcgctcgac gcagtgggca acacgaccaa ggcggtgacg
     2761 aagggctatg ctatcggctc tgctggtctc ggtgctctcg tcctcttcgc ggcctacacc
     2821 gaggatctcc ggtacttcac agcgcaggca gcctcggata ccacgcactt ccagtacttc
     2881 cgcggtctga atgtcgattt ctcgctgtcg aacccgtatg tggtggtcgg cctcctgttc
     2941 ggcggccttc tgccctacct gtttgctgcc atgggcatga cggcggtggg acgcgcggcg
     3001 agctccatcg tggaggaagt tcgcaggcag tttcgcgaga agcccggcat catgcaaggc
     3061 accgagaagc cggactacgg cagggccgta gacatgctga cgaaggcagc gatcaaggaa
     3121 atggtcattc cgtcgctgtt gccggttctg tccccgattg tgatcttcgt gctgatcacc
     3181 tttatcggcg gcaaggccgc aggcttcgcg gcgctcggcg cgatgctcct cggcgtgatc
     3241 gttacgggcc tgttcgttgc gatctcgatg acatcaggcg gcggcgcttg ggacaacgca
     3301 aagaaatcct tcgaggacgg cttcgtcgat aaggacggca ggcgtcacat gaagggttcc
     3361 gaagcgcaca aggcttccgt gaccggcgac accgtcggcg atccatacaa ggatacggct
     3421 ggccccgctg ttaaccccat gatcaagatc acgaacatcg tggcgctctt gatcctcgcg
     3481 gttctcgcgc atcagtagga tctcgcaaac ctcaagacta taagggccgc atctggagcg
     3541 gcccttattt gttatggcaa tgcaccaatt agatgtttgt catg
//
LOCUS       DATMGX010000680         2319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_301711, whole genome shotgun sequence.
ACCESSION   DATMGX010000680 DATMGX010000000
VERSION     DATMGX010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301711"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /gene="nadE"
                     /locus_tag="VNR65_11175"
     CDS             complement(<1..835)
                     /gene="nadE"
                     /locus_tag="VNR65_11175"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HWJ19277.1"
                     /translation="MSTTFSAAALELDAAAACAEIETAIHDQVLRELRRKGVVLGLSG
                     GIDSSVVASLCVRALGAERVFALFMPEEESAKESLELGALLASKLGVSSATESITPIL
                     KAAGCYRRRDEAIRSVIPEYREGWKSKLVATGGGRYSFFKIVVESPEGVRKSARLSLP
                     AYLGVVSATSFKQRTRIMLEYYHADRLNYAVAGTPNRLEYDQGFFVKSGDGAADLKPI
                     AHLYKRQVYQLAEWLGVPEEIRRRPPTTDTYSLPQSQEEFYFCLPFDVLDLCLLGKDH
                     GI"
     gene            complement(832..>2319)
                     /locus_tag="VNR65_11180"
     CDS             complement(832..>2319)
                     /locus_tag="VNR65_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ19278.1"
                     /translation="IFDRRRITYGEIETCSNRLAHALIAQGLQHGDRVAIWLENSPDV
                     AIALFAVLKAGGVFLVLNSTTKGDKAEHILNDCRARALFTDGHRAHEFRGHWSAVPHL
                     EFVWSSEASLNGDIQTPGKSVASLPAALADEGLPARPPEKGRIDIDLAALIYTSGSTG
                     KPKGVMLTHRNMVSAAISITTYLENTCDDIILSTLPLSFDYGLYQVLMAFLVGATLLL
                     EPSFAFPHRVLEVARRERVTGLPIVPTISALLLQMDLSRYDLSSLRYITNTAAALPAE
                     HIRRLRDLLPRVRIYSMYGLTECKRVSYLPPDQIDVRPTSVGRGMPNEEVYVVDERGC
                     RVGPGIVGELVVRGSNVMKGYWEDPAETAKCLRPGRYPSEKVLYTGDLFKTDSEGYLY
                     FVGRKDEMIKSRGEKVSPREIEEVLYSLDGVVEAAVVGVPDPVLGQAIKVFITRRAES
                     KITERAVLQHCAQRLEDFMVPRAVEFHDCLPKTPSGKIDKREMVQ"
BASE COUNT          477 a          725 c          644 g          473 t
ORIGIN      
        1 aaatgccatg gtcttttcct aacaggcaaa ggtccaggac atcgaaggga aggcaaaagt
       61 agaactcttc ctgtgattgc ggcaacgaat aagtgtccgt ggtgggcggc cgccggcgga
      121 tttcctcggg cacccccaac cattctgcga gttggtacac ctgccgcttg tacagatgag
      181 caatgggctt caggtcggcg gcgccgtcac cggatttcac gaaaaatccc tgatcgtact
      241 ccaggcggtt gggagttccg gctaccgcgt aattgagccg gtcggcgtgg tagtactcca
      301 gcattattcg ggtgcgctgt ttgaagctgg tggcggaaac cacccccagg tacgccggga
      361 gactgagccg ggcagacttg cggacaccct cgggcgactc tacaacgatc ttgaagaacg
      421 aatatcttcc tccgccggtg gccaccagct tcgacttcca accttcccga tactcgggta
      481 tgacgctgcg gatggcttca tcccggcggc gatagcaccc ggcggctttc agaatcggcg
      541 tgatcgattc ggttgccgaa cttactccaa gtttcgaggc aagcaacgcg cccaactcaa
      601 gactttcctt cgccgactcc tcttccggca tgaacagcgc gaacaccctc tccgcgccca
      661 gcgcgcgaac gcacaggctt gccaccacac tactgtcgat accgcccgac agcccaagca
      721 cgactccctt gcgacgtagc tctcgcaaca cttggtcatg gattgccgtc tcgatctcgg
      781 cgcaggccgc agccgcatcc aattcaagcg cagcggcaga gaaggtcgtg ctcactgcac
      841 catctcccgt ttgtcgatct tgccgctcgg tgtcttcggc aggcaatcgt gaaactccac
      901 cgcccgcggc accatgaaat cctcaagccg ctgtgcacag tgttgcagca ccgcccgttc
      961 ggttatcttg ctttctgccc ggcgtgtgat gaagaccttg attgcctgcc ccagcacggg
     1021 gtccgggacg cccaccacgg ccgcctcgac gacgccgtcg aggctgtaca gcacttcctc
     1081 gatttccctt gggcttacct tttcgccgcg gcttttgatc atttcgtcct ttctcccgac
     1141 gaagtagagg tatccctcgg aatccgtctt gaaaaggtca cccgtataaa gaactttctc
     1201 cgatggatat cggcccggac ggaggcactt ggccgtttcc gccggatctt cccaatatcc
     1261 cttcatcacg ttggatccgc gaaccaccag ttcgccaacg atgccggggc ccacccgaca
     1321 gcctctttca tcgacaacgt agacttcttc gttgggcatg ccacgcccca ccgacgtcgg
     1381 ccgaacatca atctgatccg gtgggagata cgagacacgt ttgcactcgg tcaggccata
     1441 catcgagtag atccgcactc gcggcaacag atctcgcagc cgacgaatgt gttctgccgg
     1501 cagggctgcc gcggtattcg tgatgtagcg cagggaggac agatcgtagc gactgaggtc
     1561 catctgcaac aacagcgcag agattgtcgg aacgatgggc agtcctgtga cacgttcccg
     1621 cctcgcgact tccagaacgc gatgtggaaa ggcgaatgac ggttccagaa gcaacgtggc
     1681 accgactagg aaggccatta ggacctggta aaggccgtaa tcgaacgaca gagggagagt
     1741 actcaggatt atgtcatcgc aggtgttctc cagataagtc gtaatcgaga tcgcggccga
     1801 aaccatgttg cggtgcgtca acatgactcc tttcggcttt cccgtggaac ccgacgtgta
     1861 aatcaatgcc gcaagatcga tatcaatacg gcccttctcc ggcggacgtg ccggcaggcc
     1921 ctcgtcggcc agggctgcgg gcaggcttgc taccgacttg cccggtgttt gaatatcgcc
     1981 gttcagggac gcctctgaac tccacacgaa ctcgaggtga ggtacggctg accaatgccc
     2041 gcgaaactca tgggcacggt ggccatcggt gaacagcgcc cgggcccggc agtcattcag
     2101 aatgtgctcc gccttgtctc ccttggtcgt tgagttcaac accaggaaca cacctccggc
     2161 cttgagtaca gcgaagagcg cgatggccac atcgggggag ttttccagcc aaatcgctac
     2221 gcgatcgccg tgctgcaatc cctgcgcgat gagcgcatgc gccagccggt tcgaacaagt
     2281 ttcaatctcg ccgtacgtga tgcgccgtcg gtcgaagat
//
LOCUS       DATMGX010000681         2203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_414399, whole genome shotgun sequence.
ACCESSION   DATMGX010000681 DATMGX010000000
VERSION     DATMGX010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414399"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..814)
                     /locus_tag="VNR65_11185"
     CDS             complement(266..814)
                     /locus_tag="VNR65_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWJ19279.1"
                     /translation="MKRVKRIPAAVLFAAATFIGCQQASAECGIASTYAGGSRTANGE
                     HYNHMGVSAAHKSLPFGTRVVVRNQRTGRSITVRINDRGPFVGGRIIDLSTGAKNALG
                     MDGLASVCLEVVSYGSGKKGFRNNELSEPLQEANATQARQGVRVARHQARSTAKVRYA
                     HKGRSHYAHRSEHRGRRKYASN"
     gene            1481..>2203
                     /locus_tag="VNR65_11190"
     CDS             1481..>2203
                     /locus_tag="VNR65_11190"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWJ19280.1"
                     /translation="MSAATAKHHPSQAPHSQEGKFFSAPLREADPEIFGVLESELLRQ
                     QDEIELIASENYVSRAVLEAAGSVLTNKYAEGYPGKRYYGGCQVVDVAEELAINRAKQ
                     MFGCDFANVQPHSGSQANQGVFLAVLKPGDTILGMGIDAGGHLTHGAAPNMSGKWFHA
                     VHYGVRREDGFIDYEQVERLAREHKPRLIIAGGSAYARIIDFKRFRQIADEVGALLLV
                     DMAHFAGLVAGGAHPSPFPHAHI"
BASE COUNT          489 a          633 c          569 g          512 t
ORIGIN      
        1 gccgctgatg ttgaacagcc aagcgcaaag ctgaacggaa tcatatagat cagacggagt
       61 aaactcatcc gaagtcattt cctcaagatt gtcagtgtca attgtatcaa catgtacaac
      121 cgagaaaagt tcgagaacta cgttaactaa tgctcaaaaa agaaattaag gaaaactttg
      181 acgccgcagg agatgtcttc caaacaagga aaaatgcggg gtgctgatat tttgacgcac
      241 cgccgcatta agttttgctt cgcttttagt tggacgcgta tttcctgcgc ccgcgatgtt
      301 cggaacgatg cgcgtaatgg ctcctgccct tatgggcata gcgcaccttg gcggtgcttc
      361 gagcctgatg acgggccaca cgaaccccct gacgcgcctg cgtcgcgttt gcctcttgca
      421 aaggctcact aagctcgttg ttccggaaac ccttcttccc gctgccgtag cttacgactt
      481 cgaggcagac ggacgcgagc ccatccatgc cgagcgcgtt cttggcgcct gtcgaaagat
      541 cgatgatccg ccccccaacg aagggaccgc ggtcgttgat acgaactgta atgctgcggc
      601 cagtccgctg attgcgaacg accacgcgtg ttccgaaagg caagctctta tgagctgccg
      661 atactcccat gtggttgtag tgttcgccgt tcgccgtgcg tgagccaccg gcataggtgg
      721 aggcgattcc acattcggca gacgcctgct ggcagccaat gaaagttgcc gctgcgaaca
      781 aaacagccgc tgggatacgc ttcactctct tcatcacaac ctttccttgt cgtgtcgatt
      841 acgaggttgt gatcaaactg gtaaatgagg cgtaaatttg gagcactggt gaattatctt
      901 gcacgcgaca tccgagccac tcagattcac gatggttacg ccttcattta tggatgcttt
      961 tctaaacttg tttcagcact ttaatgttgc acgatactcg cgattattag tttttagtcc
     1021 atttcatcgc gcagccattc gcttgatctt gaacctttgg tgcgtcaatc gaacgcacaa
     1081 ataccttgca ctttggtatc gtgactctca gcgtatttcg cccgcaagga cgcattcccg
     1141 aaggaccctg ccaaagagct gcatcaaaac tcgtgggcgc tcccttcaag gcacttcttt
     1201 tgaagaaaca acctatatcc aggcactctg gctattatac ttgcgatata catgctgtta
     1261 catatgtaac atacgtttcg caaagataaa cttgcctcat attacctggc ttttcacggc
     1321 gctttgcgga agcttcgcgt gcccacgcgc tccggggttt cgcggcgagg gcgaacctct
     1381 ggtcccctaa aacaggcggc gaaaggatca ccggcctcga ttgcattctg accggaagtt
     1441 cctatactgc gcgcacttca ttccatccca tagagaccca atgtcagccg caacagcgaa
     1501 acatcaccca tcgcaagctc cgcattctca agaaggcaaa ttcttttccg cgccgcttcg
     1561 ggaggccgat cccgaaattt tcggcgtcct cgagagcgag cttctccgcc agcaggatga
     1621 aatcgagctt atcgcctcgg aaaactacgt atcgcgcgcg gtgctcgagg cggcaggctc
     1681 ggtcctgacc aacaaatacg cggaaggcta ccccgggaag cgctattacg gcggctgcca
     1741 ggtcgtcgac gtcgccgaag aattggcgat caaccgcgcg aaacagatgt tcggctgcga
     1801 ttttgcgaat gtgcagcccc actcgggcag ccaggccaac cagggcgtct ttcttgctgt
     1861 cctcaaaccc ggcgatacca ttctcggcat ggggatcgac gcgggcggcc acctgacaca
     1921 cggggccgcg cccaacatgt ccggcaaatg gtttcatgcc gttcattatg gcgtgcgccg
     1981 cgaggatggg tttatagact acgagcaggt tgagaggctt gcccgtgaac acaagccgcg
     2041 cctgatcatc gcgggcggtt cggcctatgc gcgcatcatc gatttcaagc ggttccgtca
     2101 aatcgcggac gaggtcggcg ctcttcttct tgttgacatg gcccacttcg ccgggcttgt
     2161 cgcgggcggc gcgcatccaa gccccttccc gcatgctcat att
//
LOCUS       DATMGX010000682         2190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_339744, whole genome shotgun sequence.
ACCESSION   DATMGX010000682 DATMGX010000000
VERSION     DATMGX010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339744"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..446
                     /locus_tag="VNR65_11195"
     CDS             132..446
                     /locus_tag="VNR65_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19281.1"
                     /translation="MRNPSWRISESTSSGPVGDDAAVTDYPDRFPHIISPFYEKTLTV
                     SGMREHHTQLLDIQNALITMSEDGAIEGIRDKGLRVYSWGIHDKYLSREKTLLPALPR
                     KF"
     gene            complement(913..1659)
                     /locus_tag="VNR65_11200"
     CDS             complement(913..1659)
                     /locus_tag="VNR65_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ19282.1"
                     /translation="MRCNLLPVCYVAPPELRDLRRLLRYRNLVVGQAVQMKNRMSGLL
                     MEVGAEYSKQRLHGAKYFSELLDQLEEVPESVKDLLRLSRGALETFAATQRQLLDRLQ
                     KDPLLVKRVNLLKSIRGVGEVTALTWALEICDPRRFPSIGDAESYCGLTSALKSSADK
                     QQRGPISKQRNAHLQTVLIEAAKLAPRWNKQLAELHERELKRGDRNRATLAVARKLVA
                     YLLAVDKSGKRFEVRNPPAKEMQEEVQQVA"
     gene            complement(1551..1967)
                     /locus_tag="VNR65_11205"
     CDS             complement(1551..1967)
                     /locus_tag="VNR65_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19283.1"
                     /translation="MNIKHYIGFDVHKKSISYCVKTADGKIIEEGKLRATHQVLREWA
                     QKRTEPWHGAMEATLFSSWIYDVLKPYAAELQMGNPSMMKAIGAAKKKTNWMRARSPT
                     WCAAICCRSAMWRRRSCAICGGCCVTETWWWGRRCR"
BASE COUNT          412 a          704 c          600 g          474 t
ORIGIN      
        1 ctatcggccg ttgcgtgtcg gctgggccgt gcgtcgcggc gactttgatt cgctgcgccg
       61 aattgtccgc tggtcacaca cgctttgggg ggggcgccac aaccccatac ttgtcgtaga
      121 cgatgcaggt catgcgaaac ccatcgtgga gaatttccga atcgacgtca tctggccctg
      181 tcggtgatga cgcggccgta acagattatc cggatcgctt tccccacatc atcagccctt
      241 tttacgagaa aacgctgacc gtcagcggca tgcgcgagca ccatacgcaa ttactcgaca
      301 ttcaaaatgc cctcatcacg atgagcgagg acggtgcgat cgaaggtatc cgcgacaagg
      361 gcctcagagt ttattcatgg ggcatccatg ataagtactt gtcaagagaa aaaacacttc
      421 tccctgcact ccccagaaag ttttgaagcg cctggatgat gaagccgacc gttagcgcga
      481 cgattgaccg tttgcgatct ctttggaaag gggtcaacgc atcagcggga ccagacatcc
      541 atttgcggga acagcataac gccgtccacg aagattgaat ttgggggaac cggccggcct
      601 cgaaacagac cagttggcac atcgggagga gggcctccgt cgtgcagtcc cagggaagtg
      661 aggaacggcc ggtggatcgc tgatcaatcg ttgcgtcgct acacactgcc gggtctgggt
      721 gattttcccg ccggagccgc ccgtcgcatc gatatttgcg acggaacggc ccggagccgt
      781 ccagggcgcg cccggttttg gcgcggcgta gcgaaccctt gacggcgagg accgttctgg
      841 catcgaactc ctcgcgacgg gcggctttgg tgggaaaatc acctttcccg ctggtgagct
      901 cagagcgtgt ttttaagcca cttgctggac ctcctcctgc atctcctttg ccggcgggtt
      961 gcgcacctcg aaccgcttgc ccgatttgtc caccgccaac aaatacgcca ccagcttccg
     1021 cgccaccgcc aaggtcgccc ggttgcgatc gccgcgcttc aactcccgct cgtggagttc
     1081 ggccaactgt ttgttccacc gcggcgccag tttcgccgcc tcgatcagca ccgtctgcaa
     1141 atgcgcgttg cgctgcttgc tgatcggccc ccgctgctgt ttgtcggccg acgacttcag
     1201 cgccgaggtc aatccgcagt agctctccgc atcccctatt gaggggaacc gccgcgggtc
     1261 gcagatctcc aacgcccagg tcagcgccgt cacctcgccc acgcctcgga tgctcttcag
     1321 caggttcacc cgcttcacca gcagcggatc tttctgcaat cggtcgagca gctggcgctg
     1381 cgtcgcggcg aacgtctcca gcgccccccg gctcagccgc aacaaatcct tcaccgattc
     1441 cggcacttct tccaactggt ccagcaactc gctgaaatat tttgccccgt gcagtcgctg
     1501 tttgctgtat tccgctccca cttccatcaa cagcccgctc attcggttct tcatctgcac
     1561 cgcctgcccc accaccaggt ttcggtaacg cagcaaccgc cgcagatcgc gcagctccgg
     1621 cggcgccaca tagcagaccg gcagcagatt gcagcgcacc aggtcggcga tcttgcgcgc
     1681 atccagtttg tcttcttctt ggccgcgccg atcgctttca tcatcgaggg gttccccatt
     1741 tgcaactcgg cggcataggg tttcagcacg tcgtaaatcc aactgctgaa cagcgtcgct
     1801 tccatcgcgc cgtgccacgg ttccgtgcgc ttctgtgccc actcccgcag cacctggtgc
     1861 gtggcccgca acttgccctc ttcgatgatc ttgccgtcgg cggtcttgac gcagtagctg
     1921 atgcttttct tgtgaacgtc gaatccgata tagtgtttga tgttcattgg gttctcctga
     1981 ttgaggtttc tgagccggtt cgcaaagacc ggtctcggca ttataccctt caggagaacc
     2041 cgacagcacc atacttatac attcaaacag acagccagtt tccggcaaac tgcgccggaa
     2101 acttggtgtc agtcccgggg tttgcggcat gacgccgacc acctccgcaa aatcggtgag
     2161 gcacagggag aagggatgac gaaacgtttg
//
LOCUS       DATMGX010000683         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_87749, whole genome shotgun sequence.
ACCESSION   DATMGX010000683 DATMGX010000000
VERSION     DATMGX010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87749"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /gene="ltrA"
                     /locus_tag="VNR65_11210"
     CDS             complement(<1..1216)
                     /gene="ltrA"
                     /locus_tag="VNR65_11210"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HWJ19284.1"
                     /translation="MNPQSKPFLIDKRLVYEAYLEVRSKEGAGGVDGVTIEQFEKDLK
                     GNLYKVWNRLSSGTYFPPPVRAVTIPKKNGGERILGVPTVGDRVAQTVVKQMIEPDLD
                     KIFLADSYGYRPDKSALDAVGVTRERCWKYDWVLEFDIKGLFDNIDHSLLMRAVRKHV
                     TCPWALLYIERWLTASMLREDGTLVERTRGTPQGGVVSPVLANLFLHYTFDLWMARAF
                     PHLRWCRYADDGLVHCRTEKEARAVWDALNARMTECRLELHPTKTKIVYCRDNRRKAN
                     YENVAFDFLGYCFRPRSVKGPVSQRMFCGYTPAVSKPALNAMRSTIRNMRLRKRTQVT
                     WDDIARELNPITRGWIAYYGRYTRSALYPLVRYIDQTLAVWIKRKYKRFHRRLGQARE
                     FLAKIARESPRLF"
     gene            complement(1775..2113)
                     /locus_tag="VNR65_11215"
                     /pseudo
     CDS             complement(1775..2113)
                     /locus_tag="VNR65_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427389.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          381 a          717 c          566 g          449 t
ORIGIN      
        1 cgaacagccg tgggctttcg cgcgcgatct tcgcgagaaa ctcgcgcgct tgacccagtc
       61 ggcggtggaa gcgtttgtac ttgcgcttga tccagacggc caatgtctgg tcgatgtagc
      121 gaaccagggg atacagcgcc gaacgggtgt atcgcccata gtacgcgatc catccccgag
      181 tgatcgggtt cagctcgcgg gcgatgtcgt cccacgtcac ctgtgttcgc ttgcgcaacc
      241 tcatgtttcg gatcgtcgac cgcatcgcgt tcagcgcagg cttgctcacc gcaggcgtgt
      301 acccgcagaa catcctctgc gataccgggc ctttgaccga gcgtggcctg aagcaatatc
      361 cgagaaagtc gaaggcgaca ttctcataat tggcctttcg ccgattgtct ctgcaataaa
      421 cgatcttggt cttggtcgga tgcagttcca ggcgacactc cgtcatccga gcgttcagcg
      481 cgtcccatac ggcccgcgcc tccttctcgg tccggcagtg caccagcccg tcatccgcgt
      541 accgacacca ccgtaggtgc ggaaaagccc gtgccatcca aagatcaaac gtatagtgca
      601 agaaaaggtt ggccaagact gggctgacga cgcctccttg cggagtaccg cgcgtccgct
      661 caaccaatgt tccgtcctct cgcagcattg aggctgtcaa ccatcgttcg atgtagagca
      721 gcgcccaagg gcacgttacg tgttttcgga cggcccgcat caacagcgaa tggtcgatgt
      781 tgtcgaacag acccttgata tcgaactcta ggacccaatc gtatttccag caccgctcgc
      841 gcgtaactcc gacagcatcg agcgccgact tgtcgggccg ataaccgtag gaatccgcca
      901 gaaagatctt gtccagatcc ggctcaatca tctgcttgac gacagtctgc gccacgcggt
      961 cgcctacggt cggaacacca aggattcttt cgccgccatt cttcttcgga atggtcaccg
     1021 cgcgcacagg gggcgggaag tacgtgccag aactcagtcg attccagact ttgtagagat
     1081 tgcccttcaa atccttctcg aactgctcga tcgtcacgcc gtcgacgccg cctgcaccct
     1141 ccttggatcg cacctccaga tatgcttcgt acaccagtcg cttgtcgatc aagaatggct
     1201 tgctttgcgg attcatcgac gtcctcctgt cgccagttgc gtcaacgatc cgccgactcg
     1261 atctgacccc ttcgctccag ccccattacg ggccttcatc gctactacgg gtcggtccgt
     1321 cccagcgccc cgcatcggta ctctcgcctc acggtcttct ccgcttgagc ttctcccttt
     1381 gcatcggggc ggctggttcc tgcggttcca cggcaacgcc tgcatccgct tcacgccccc
     1441 tcaacgccgg tcgtcgctcg cccagtcctc aggcatccgg cgagctgatc ccaggaagac
     1501 gacacatccc tggttttgac gacgttttcc acgtcacgac gcttcatcgg tgggttcgct
     1561 ttcgctcgtc tctcggacgc atacctgttc aaggtgctcc ctgaactttg cctccaacgc
     1621 tcacgaccac gcctcttaag cgcagccgct tggagtggtt tgggacctgc tcctgaaagc
     1681 cgatcccgag gggccatccc tcatcgttcc cgcagcttca gcacacagac tttcagatca
     1741 tgcagatcat cctttctgcg tgcttccgca gcacactgtc gtcgccgtgc aggcgctcgg
     1801 cagccatgac atgggcctcg acgaggcgcc ggaggggatc gagagccgcg cagaccgcgc
     1861 ccactgcgtc ggccatggtg gacagtgcga tcggcgcgcc ctccagcgca tagcgctccg
     1921 cctgccggtt cagaggttgg tgctggccga acttctcgaa cacgatcatc gccaagaggc
     1981 tcggacccgc ccatcccctc gggatcacat ggaacggcgc cggcgtctgg ctgatcgtct
     2041 cgcagtcccg gcacgagaac ttctcccgca ccgtctcgat caccttccat cgccggggcg
     2101 tcgtctccag cgt
//
LOCUS       DATMGX010000684         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_197162, whole genome shotgun sequence.
ACCESSION   DATMGX010000684 DATMGX010000000
VERSION     DATMGX010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197162"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VNR65_11220"
     CDS             <1..189
                     /locus_tag="VNR65_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19285.1"
                     /translation="GQCQDLNLVKKIARMAADPRSDRPYDPPKIIAIKIIDPRHPAAK
                     PGTAAHKPTAKPATPPSQ"
     gene            complement(253..1623)
                     /locus_tag="VNR65_11225"
     CDS             complement(253..1623)
                     /locus_tag="VNR65_11225"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HWJ19286.1"
                     /translation="MKRTDNMPTSKKQGNTNTAKGEIAPAKGKLGIMIPGMGAVATTF
                     VAGVEAVRKKIASPIGSLTQMGTVRLGKRTEGRFPKINEFVPLAGLNDLVFTGWDIFE
                     DDMYSAAGNAGVLERSLLEQVKPFLSSIKPRKAVFDKNYVKKLDGPNVKKGKNKMELA
                     EQLRDDIREFKQSSGAKRLVMIWCGSTESFIKAGPVHQSLAAFEKGLRASDENIAPSM
                     IYAYAALKEGVPFANGAPNLTVDIPVMHDLSKQMEAPICGKDYKTGQTLIKTILAPGF
                     KARMIGLNGWFSTNILGNRDGEVLEDPGSFKTKEESKLSVLEHILQPELYPELYGHIC
                     HKVRINYYPPRGDNKEGWDNIDIFGWLGYPMQIKVDFLCRDSILAAPLVLDLVLFLDL
                     AKRSSELRGIGIQEWLSFYFKSPMTAPGLYPEHDLFIQSMKLKNTLRHLKGEEMITHL
                     GLEYYD"
     gene            1738..>2030
                     /locus_tag="VNR65_11230"
     CDS             1738..>2030
                     /locus_tag="VNR65_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ19287.1"
                     /translation="MAREKVAIVTGSSSGIGLLTAVELALNGYRVVATMRNLESRGRL
                     EEAAQKAGVRDRLDLRRLDITEFDSLPGAVDAMVRDHGRVEVLVNNAGFSVAG"
BASE COUNT          420 a          575 c          591 g          444 t
ORIGIN      
        1 ggacagtgcc aggacctcaa tttggtgaag aagatcgcac gcatggcggc cgatcctcgc
       61 agcgatcggc catacgaccc gcccaagatc atcgccatca agatcatcga cccgcgtcat
      121 cccgcagcga agccgggaac cgcggcccac aagccgacgg cgaagcccgc gactccgcca
      181 agccagtaac gtgccgtgga ggttgcaagg aactcaggcg aggatctcct cgccttgctt
      241 ccgaatcaag cctcagtcgt agtactcgag ccccagatgg gtgatcatct cttcgccctt
      301 cagatggcgc agggtgttct tcagcttcat ggactggatg aacaggtcat gctcgggata
      361 caaccctggc gccgtcatcg gcgatttgaa gtagaagctc agccattcct gaatgccaat
      421 accacgcagc tccgacgagc gcttcgccaa gtcgaggaac aacaccaggt cgagcacgag
      481 cggagcagcg aggatagagt cgcggcacag gaaatcgacc ttgatctgca tcggatagcc
      541 gagccatccg aagatatcaa tgttgtccca accctcttta ttatcaccgc gtggcgggta
      601 atagttaatg cgaaccttgt ggcagatgtg gccgtaaagt tcgggataaa gttccggctg
      661 aagaatgtgt tccagtaccg acagcttcga ctcttccttg gtcttgaatg aaccaggatc
      721 ttcgagcact tcgccgtcgc gattgcccag aatgttggtg gaaaaccatc cgttcaggcc
      781 gatcatgcgc gctttgaatc ccggcgcgag gatcgtcttg atcaacgtct ggccagtctt
      841 gtagtccttg ccgcagatgg gtgcttccat ttgctttgac agatcgtgca taacagggat
      901 gtcgaccgta aggttcggcg caccattagc gaaaggcaca ccttccttca gcgcagcata
      961 ggcgtaaatc atcgaaggcg cgatgttctc gtcgcttgcc cgcagaccct tctcaaacgc
     1021 cgccagggat tgatgcaccg ggccggcctt gatgaaggat tcggttgagc cgcaccagat
     1081 catcaccagg cgcttggccc cgcttgactg cttgaactcg cggatatcgt cgcgcagttg
     1141 ctcggcaagc tccatcttgt tcttgccctt cttcacgttg gggccatcga gcttcttgac
     1201 gtagttcttg tcgaacacgg ctttgcgagg cttaatcgag gaaaggaagg gctttacctg
     1261 ctccagcagc gagcgctcga gaacgccggc gttgcccgcg gcggaataca tgtcgtcctc
     1321 gaagatgtcc cagccggtga agacgagatc gttgagcccg gccagcggca cgaattcgtt
     1381 gatcttcggg aagcggccct cagtgcgctt tcccaggcgg acggtgccca tctgggtcag
     1441 cgatccgata gggctggcaa ttttcttgcg caccgcttcg acgcccgcaa caaacgtagt
     1501 ggcaaccgcg cccataccgg ggatcatgat cccgagcttt cctttggcgg gcgcgatctc
     1561 gcctttggcg gtattcgtat ttccctgctt cttgctggtc ggcatgttat cagtccgttt
     1621 catgaaagaa tgtacaaacc tgttatcttc tcgtatctgt actgccattg caattgaagg
     1681 cgtgcgaatg ggccggtttt accgttttgc agcaatctga aggatttggc gggagttatg
     1741 gctagggaaa aagtcgcgat tgtgaccgga agttccagtg gcattggact gctaaccgcc
     1801 gtcgagttgg cgctcaacgg ctatcgcgtg gtggccacca tgcgcaatct cgaaagccga
     1861 ggccgtcttg aggaagcagc gcaaaaggcc ggcgtacgtg accggctcga cctgcgccgg
     1921 ctggacatca cggaattcga ttcgctgccg ggcgcggtgg acgcgatggt ccgcgaccat
     1981 ggacgcgttg aggtgttggt gaacaatgcg ggattcagcg tggcaggttt
//
LOCUS       DATMGX010000685         3103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_333810, whole genome shotgun sequence.
ACCESSION   DATMGX010000685 DATMGX010000000
VERSION     DATMGX010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333810"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /gene="nifN"
                     /locus_tag="VNR65_11235"
     CDS             <1..1270
                     /gene="nifN"
                     /locus_tag="VNR65_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HWJ19288.1"
                     /translation="PLLHGSQGCTSFGLVLFVRHFREAIPMQTTAMSEVATVLGGYEN
                     VEQAVVTIANRTNPAIIGVCSTGVTEIKGDDVDGFIKLIRQSRPELDHLKLVFVSTPD
                     FDGAFQDGWAKTVTRMVEQLVETPPLGAARNTQRVNLLPGCHITPGDLDELRDIFEAF
                     GLEPLILPDLSGSLDGHMPDDFTPTTLGGISVEDIASMGRSAWTIAIGEQMRDAAVAL
                     EKKASVPFRLFDRLTGLAPNDAFIAFLSKISGRPVPNKFRRQRSQLLDAMLDGHFSFG
                     NKKIAVGAEPDLLFSVSTWLTEMGCTIEAAVTTTQSPMLEKVPADEVLIGDLEDLENR
                     AADCDLIVTHSHGRQMSERLGVPLFRYGLPTFDRLGAAHKLSVGYRGTRNLIFEIGNI
                     FMAEVHEPTPDIWRNPRSEEDHHAAPVTAH"
     gene            1245..1739
                     /gene="nifX"
                     /locus_tag="VNR65_11240"
     CDS             1245..1739
                     /gene="nifX"
                     /locus_tag="VNR65_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifX"
                     /protein_id="HWJ19289.1"
                     /translation="MPRRLRLIRAVRIATNADETKERAGTVKIAFATEDLKRVDAHFG
                     WAKNIAVYELSDEECKLVKVFQFEGDLKEDGNENKLTPKVEAVMDCAILYVASIGASA
                     AARIVQKGIHPIKVNQPEEIADLLAKLQAVLKGNPPPWLRKALLKGKEREFDFDEEDE
                     GAHA"
     gene            1732..2199
                     /locus_tag="VNR65_11245"
     CDS             1732..2199
                     /locus_tag="VNR65_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HWJ19290.1"
                     /translation="MPETAKVETAPVPDSIFLNELIKQWRAQDAHGAWDKKSDLDLIA
                     PYIITKEQRREVPIIGDPDPETLWRLELFYNAVGLAIERATGIMVSPIMKMHHEGFGR
                     MVLTAGRLVVVNKHLRDVHRFGFETLGKLAEEGDKLVRAGVEMIEKFPEVAKY"
     gene            2211..2411
                     /locus_tag="VNR65_11250"
     CDS             2211..2411
                     /locus_tag="VNR65_11250"
                     /inference="COORDINATES: protein motif:HMM:NF016938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HWJ19291.1"
                     /translation="MSEIDALKAEIKKLSAKATQAKMDLHDLSEELPINWQQIPEVAQ
                     RAHDAYAILEKKRAQLKHLETA"
     gene            2436..2759
                     /gene="fdxB"
                     /locus_tag="VNR65_11255"
     CDS             2436..2759
                     /gene="fdxB"
                     /locus_tag="VNR65_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HWJ19292.1"
                     /translation="MDSETRDGRGWEPEYLIAIDSKTCIGCGRCFKVCGRNVMTLKGL
                     TEDGELVAIDASSDEDDDDYDDDEFERKIMTMADAGACIGCGACARVCPRACQSHSKA
                     PPPLG"
BASE COUNT          654 a          948 c          941 g          560 t
ORIGIN      
        1 gccgctgctt cacggttcgc aaggctgcac ctcgttcggg ctcgtcctct tcgtgcgcca
       61 tttccgcgaa gccatcccga tgcagacgac tgccatgagc gaagtcgcga ccgtgctcgg
      121 cggctacgag aacgtcgagc aggctgttgt caccatcgca aaccgcacga accctgccat
      181 cataggcgtc tgctcgaccg gcgttaccga gatcaagggc gacgacgtgg atggcttcat
      241 caagctcatc cgccagagtc gtcccgaact cgatcatttg aagcttgtct ttgtctccac
      301 gcccgacttc gacggcgcct tccaggatgg ctgggcgaaa accgtgaccc gcatggtgga
      361 gcagcttgtc gagacgccgc cgcttggcgc ggcccgaaat acacagcgcg tcaacctact
      421 gccgggctgc catatcacac ccggggatct ggacgagctg cgcgacattt tcgaagcatt
      481 cggcctcgaa ccgctgatcc tgccagacct gtccggctcg ctcgatgggc atatgcccga
      541 cgacttcacg ccgacgacgc tgggcggcat ttccgtggaa gatatcgcaa gcatgggccg
      601 ctccgcgtgg accatcgcta tcggcgaaca gatgcgcgat gcggcggtgg ccttggaaaa
      661 aaaggcaagc gttcccttcc ggcttttcga tcgcctgacc ggcctcgcac cgaatgatgc
      721 tttcattgcg tttctgtcga aaatcagcgg ccgcccggtg ccgaacaagt ttcgccgcca
      781 gcgcagccag cttctcgacg cgatgctcga cgggcatttc tccttcggta acaagaagat
      841 cgctgtcggc gcggagcccg atcttctctt cagcgtctcg acctggctca cggagatggg
      901 ctgcacaatc gaggcggccg taacgacgac gcaatcgccg atgctcgaaa aagtgccagc
      961 agacgaggtg ctgatcggcg acctcgaaga cctcgaaaat cgcgccgccg actgcgatct
     1021 gatcgtgacc catagccatg ggcggcagat gtccgaacgg cttggcgtcc cgctgttccg
     1081 ctacggcctc cccaccttcg accggctcgg cgcggcccac aaactttcgg tcggttatcg
     1141 cggcacgcgc aatctcattt tcgagattgg gaatatcttc atggcggagg ttcacgaacc
     1201 gacgccggac atatggcgca atcctcgcag cgaggaggat catcatgccg cgccggttac
     1261 ggctcattag ggcggtacga atagcgacga atgcagacga gacaaaggaa agggccggca
     1321 cagtgaaaat cgcctttgca actgaggacc tgaagcgcgt ggacgcgcat ttcgggtggg
     1381 ccaagaacat cgcggtctat gagctttccg acgaggagtg caagctcgtc aaagtctttc
     1441 agttcgaggg cgacctcaag gaggacggca acgagaacaa gctgaccccc aaggtcgagg
     1501 cggtcatgga ttgcgccatc ctctacgtcg cctccatcgg cgcgtcggcc gctgcccgca
     1561 tcgtccagaa gggcatccac ccgatcaagg tcaaccagcc cgaagagatc gcggacctcc
     1621 tcgcaaaact tcaggcagtg ctcaaaggca atccgccgcc ctggctgcgc aaggcgcttc
     1681 tgaagggcaa ggaacgcgaa ttcgatttcg atgaagaaga cgagggtgcc catgcctgag
     1741 acagccaaag tcgaaaccgc gcctgttcct gactcgatct ttctgaatga gctgatcaag
     1801 cagtggcgcg cccaggatgc acacggcgcc tgggacaaga aaagcgatct cgatctgatc
     1861 gcgccctaca tcatcaccaa ggagcagcgc cgcgaggtcc ccatcatcgg cgaccccgac
     1921 cccgagacgc tgtggcggct ggaactcttc tacaacgccg tcggtctcgc catcgagcgc
     1981 gcgaccggca tcatggtctc gccgatcatg aagatgcatc acgaaggctt cggacgcatg
     2041 gtgctgactg cgggccggct cgtcgtcgtg aacaagcatc tgcgcgacgt gcaccgcttc
     2101 gggttcgaga cgctcggcaa gctcgccgag gaaggcgaca agctcgtccg ggctggcgtg
     2161 gagatgatcg agaaattccc tgaagtcgca aagtattgag cgagggacgc atgagcgaaa
     2221 tcgacgccct caaggcggaa atcaagaaac tctcggccaa ggccacgcag gccaagatgg
     2281 atctgcatga cctgtccgaa gaactgccga tcaactggca gcagatccct gaagtcgcgc
     2341 aacgcgcgca tgatgcgtat gcgatcctgg aaaagaagcg cgcgcagttg aagcatctgg
     2401 agaccgcgtg agccgcggag tttaaggaga gggcgatgga tagtgagacg cgcgacggga
     2461 ggggctggga gccggaatac ctgatcgcga tcgatagcaa gacctgcatc gggtgcgggc
     2521 gttgtttcaa ggtttgcggc cgcaatgtca tgacgctgaa aggtctgacg gaggacggcg
     2581 agcttgtggc tatcgacgcc tcctccgacg aggacgacga cgattacgat gacgacgagt
     2641 tcgagcgcaa gatcatgacc atggccgacg ccggggcgtg catcggctgc ggagcctgcg
     2701 cgcgcgtctg tccgcgggca tgccagagcc actcgaaagc gccgccgccc ttgggttaac
     2761 cgatgaaatt agaaagtcgc ccctcatcct gtccttctcc cggcgaactc gccaaggcga
     2821 gttcgcaact caacatgatc gcatccgcaa gcggatgcga tgtgggagaa gggacgccgt
     2881 tgcaatcggc acaacctgct ccctctcccc ttgcgggaga gggctggggt gagggggttc
     2941 gacgttttgc gaatcaatga ttctgggtgc tagccaggtt ggaccaccct tgtatggatc
     3001 gcgacattcg ccgtctcccg atggtccgta acgcgccaac agtccgacaa acttaggtct
     3061 gctcgtcttg cgcttttccc gccgcactga tactcctgca tag
//
LOCUS       DATMGX010000686         3080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_36765, whole genome shotgun sequence.
ACCESSION   DATMGX010000686 DATMGX010000000
VERSION     DATMGX010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36765"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1465
                     /locus_tag="VNR65_11260"
     CDS             194..1465
                     /locus_tag="VNR65_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ19293.1"
                     /translation="MEVMHERVAGLDVHKETVVCCVRIVAGGKVSRECRSFETTTAGL
                     EALLAWLTQSGCTHVAMEATGVYWKPVWNILSDGKFELILANAAHIKNVPGRKTDVSD
                     AAWIADLAAFGLIKASFVPEEAIQDLRSLMRARKQLVREQTRHIQRIQKTLEEANIKL
                     DSVISDIMGVSGRRMIEAMIQGVRSPQKLALLADRRIKASPKALYDALHGRLTDHHRF
                     MLRLHLGQYDALASSIAEIDGQVDEAIARIDEEAAAGRGSFRELTALLDTIPGIASLA
                     ATSILAEIGTDMSRFPTAGHLVSWAGLSPGQNESAGKRKSSRLRKGAPWLKTLLVQCA
                     WAAIKKKDSYYRAQFNRLKARRGPKKAICAVAASMLTAIYHMLKDGVEHNDLGAGHFD
                     RRPAVIKANRLVAQLAKLGFKVELQPLAEAA"
     gene            complement(1536..2606)
                     /locus_tag="VNR65_11265"
     CDS             complement(1536..2606)
                     /locus_tag="VNR65_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19294.1"
                     /translation="MRYLALLLAAAGGLFSCERAEAAFTKAFQSGSWEGTAYYRDDNG
                     AVSVCSARTDYENGFSLSLALRPSGSWALLLVRPEGFSHPPSQYTLYADGKVIHSGPG
                     RPESNGQLLRIDIPYSEETVQSLQHGTTLRISSSYGDTAFSLKGSADAIAELRSCVKA
                     EGKGTFGSGWEKDQSSLVDSKPAAKNEADAPRPVTRDQLIPYANQILQEAGFADYRFL
                     PQSTGEKSNALIWQFKDGTLGSLAAAEKAGSIDLDRYIGQMTIDDTVSCKGEFASGKR
                     APRYVNGAEVRKVFASCNSGPRSFYAEYALVRMPNGFLVKLTSLKEGSSVMFGRGSRE
                     AQDDLDKVTRTEESTLAVFSKH"
     gene            complement(2818..>3080)
                     /locus_tag="VNR65_11270"
     CDS             complement(2818..>3080)
                     /locus_tag="VNR65_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWJ19295.1"
                     /translation="EREHLYWQLEALVAPYDAILTPSSTGPAPLGLHATGNPVLCTLW
                     TFFGVPAMNLPLLTVDGLPLGVQLTGLRFEEERLFRAAASLC"
BASE COUNT          629 a          900 c          910 g          641 t
ORIGIN      
        1 cttttataat agggtttgcc gtcagcaaag aggccggctt ctctatggtg cgcaatagcc
       61 cgtctgttag ttgggtccgg ggcgcctcga ttacccccaa tggagccaag gagccgcaac
      121 gctcccggag cacgtttctt agaattcttt ttcgacgggt tccccacgct ggcggcagcc
      181 gagggagaac gtaatggaag tcatgcatga gcgtgtcgcc gggcttgatg ttcacaagga
      241 gacggtcgtt tgctgtgtgc gcattgttgc tggcgggaag gtttcgcggg aatgccggag
      301 cttcgagacg accacagccg gcctggaggc cttgctggct tggttgacgc agagcggatg
      361 cacgcatgtg gcgatggagg ctacgggtgt ttattggaag cctgtgtgga acatcttaag
      421 cgatggcaag ttcgagctga ttttggccaa tgcggcgcat atcaagaacg ttcctggccg
      481 caagaccgac gtgagcgacg ccgcctggat cgccgatctg gcggcctttg gcctgatcaa
      541 ggcgagcttt gtcccggaag aagcgatcca ggacctgcgc tcgctgatgc gggcgcgcaa
      601 gcagctggtc cgcgagcaga cgcggcacat acagcggata caaaaaacgc tcgaagaagc
      661 caacatcaag ctcgattcgg tgatcagcga catcatgggg gtgtccgggc ggcggatgat
      721 cgaggcgatg atccagggcg tgcgcagtcc ccaaaagctg gcgctattgg cggaccggcg
      781 gatcaaggcc tcgcccaaag ccctctacga tgccctgcat ggccggttga cggatcatca
      841 ccgcttcatg ctgcggcttc acctcggtca atacgacgcc ttggcctctt ccatcgcgga
      901 gatcgacggc caagtggatg aggcgatcgc caggatcgat gaggaggcgg ccgctggccg
      961 cggctccttt cgcgagttga ccgcgctttt ggataccatc cctggcatcg ccagcttggc
     1021 ggcgacctcg atccttgccg agatcggaac cgatatgagc cgctttccaa cggctgggca
     1081 tcttgtgtcc tgggcgggcc tgtctcccgg ccagaatgaa agcgccggca aacgcaaatc
     1141 ctcgcggctg cgcaaggggg cgccctggct caagacgctg cttgtccaat gcgcctgggc
     1201 cgccatcaag aagaaggaca gctactacag ggcgcagttc aatcgcctca aggccaggcg
     1261 cggccccaag aaagcgatct gcgctgtagc cgcctcgatg ctcacggcga tctatcacat
     1321 gctgaaagac ggggttgaac acaacgatct gggggccggc catttcgacc gccgtccagc
     1381 cgtaatcaaa gcgaatcgcc tggttgctca actcgccaag cttggtttca aggtcgaact
     1441 tcaacctcta gcggaggccg cttgatggca ttcaaaaacc agtggctgcg cctgctccgg
     1501 ccgcccagcc gcgttgcggc cgggtgagtt tcttcttagt gtttcgagaa aacggcgagc
     1561 gtcgattctt ccgttcgggt caccttgtcg aggtcatctt gcgcctcccg gcttcctctg
     1621 ccgaacatca cgcttgaacc ctcctttaac gaggtgagct tgacaagaaa gccgttcggc
     1681 atgcgtacaa gcgcatattc cgcataaaac gaccgaggcc cggagttgca agacgcgaaa
     1741 accttcctga cctcggcccc gttcacgtaa cgcggtgcgc gcttcccgct ggcgaactcg
     1801 cccttgcaac tcactgtatc gtcaatggtc atctggccga tgtaacggtc aaggtctatg
     1861 gaccctgctt tctccgccgc ggcgagtgac ccaagcgtac catccttgaa ttgccagatc
     1921 agggcattcg acttttcgcc tgtgctttgc ggcaggaacc ggtaatcggc aaagcccgct
     1981 tcctgaagaa tttggtttgc atagggaatg agctgatcgc gggtcacggg gcggggcgcg
     2041 tcggcttcgt ttttcgccgc tggctttgag tcaacgaggc tgctctgatc cttctcccat
     2101 ccggagccga aggtgccttt cccttcagct ttcacgcagc tgcggagttc ggctattgcg
     2161 tccgcgcttc ccttcaggct gaacgcggta tcgccgtagc tcgatgagat gcgaagcgtc
     2221 gttccgtgct gcagactttg tacggtctcc tcggaatagg gaatatcgat tcgaaggagt
     2281 tggccgttgc tctccggcct gcccggcccg gaatggatga ccttgccgtc agcgtaaaga
     2341 gtgtattggc taggggggtg cgagaagcct tcaggtctca caagcaacag cgcccagctc
     2401 ccactgggcc gaagggcgag tgataggctg aacccattct cgtagtccgt tctcgcggag
     2461 catacggaga cagctccgtt atcgtccctg taataggctg tgccttccca tgagcctgat
     2521 tgaaaagcct tcgtgaaggc ggcctcggct ctctcgcagg aaaacaggcc cccggccgca
     2581 gccaaaagta aggcaagata acgcatacaa cctcacaaca acacacgaca accaagcccg
     2641 cgagtaaatc gcgagaattc gcacaccggc cgccatcatt attattgtgg gcttgccgcc
     2701 cgccttacgc tctccacttc gccttgaaga gcgcacgcac caagagtacg gactaccata
     2761 aaaagatttc tgttgataga tgtattttct gctttgatta attttttgag taagagctta
     2821 gcacaagctc gccgcggcac gaaaaagcct ttcttcttcg aatcttagcc ctgtcagttg
     2881 gacccccagc ggcaagccgt cgacggtcaa aagcggcagg ttcatggccg gtacgccgaa
     2941 gaaagtccac agagtgcata acacggggtt gcctgttgca tgcaatccga gtggcgccgg
     3001 gcccgtggaa gacggcgtca aaatggcatc atagggcgca accagggcct ccaactgcca
     3061 gtaaagatgc tcgcgttcgc
//
LOCUS       DATMGX010000687         2899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_282026, whole genome shotgun sequence.
ACCESSION   DATMGX010000687 DATMGX010000000
VERSION     DATMGX010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282026"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1091)
                     /locus_tag="VNR65_11275"
     CDS             complement(147..1091)
                     /locus_tag="VNR65_11275"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ19296.1"
                     /translation="MEMHEIKYFIVLAEELNFTRAAERCNVSQPSITRAIKMLELELG
                     GPLFHRERSNTHLSELGRMMKPYLEQVYNEAQNAKRRAAEFMNLKKTPLRLGLMCTIA
                     PDNLIDFIKAVLERHPGVPLKIADAPARQLEDDLLAGELEVAIYATPGKPLDSRLHYI
                     PLYEEDFVAVAPRGHSICAHNVVPIKSLNGQCYLSRINCEFGDSIDHVFDEQQVEWPT
                     VYESERDDWILAMVAAGLGLAFMPISSVKHDGVEARPLMPSFSRQVCLVTVRGRPYSP
                     AVGALVAEAMRSAWLSKRASAVRDMMQKEAANPEPDAR"
     gene            1375..2076
                     /locus_tag="VNR65_11280"
     CDS             1375..2076
                     /locus_tag="VNR65_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWJ19297.1"
                     /translation="MPGSEIGQDRRIRVHSRFVRATHWINAVAILIMIGSGWRIYNNM
                     PIFSWLTFPEWATLGGDPEITYKLNKDVGFSNALLWHFAAMWLFFVNGVVALGVGLAS
                     GRLQRKWAPISLSELVHDIRQALSFNLAHDDITVYNAVQKLLYSGVILATILMLVSGL
                     AIWKPVQFQALTWLFYDFQGARLAHFIGMAAIALFVLVHVMLAILVPKTITAMTRGSI
                     PAEGPAGISPVRAGE"
     gene            2079..2870
                     /locus_tag="VNR65_11285"
     CDS             2079..2870
                     /locus_tag="VNR65_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWJ19298.1"
                     /translation="MAIIRPRRSAKRPQGGVDQSALIEHRELIAGIERRAFLRGSVSL
                     GALTLLTGCDISNDSALQGALRAMSGWNDRVQAWLFDPKKLAPTYSDADVLNPPRFNA
                     FYGIDEVKKVDGATWRLELAGAIENKQPWTKDALYALPRETQITRHICVEGWDYIGKW
                     SGPPLRTFLDRIGADKTAKYVGFKCSDNYWGSIDMATALHPQTILATHYAGDPLPDAY
                     GFPVRLKTPLKLGFKQPKWIYAMEVTNVYPGGYWEDGGYNWFSGL"
BASE COUNT          609 a          862 c          831 g          597 t
ORIGIN      
        1 cgcaaaagag catgggagcg aagacgtaag cgcaaaatcc tctgcaaggt ccttttctct
       61 gccctttgca gcaaaaaagt taaagcgcct cagggcgctg ttccattatc gagtgtgcat
      121 ggaatccata gctctgagct atggcctcag cgggcatcag gctcgggatt ggccgcttct
      181 ttctgcatca tgtcccgcac cgcggacgcc cgcttcgaca gccaggcgct ccgcatcgcc
      241 tccgcgacga gcgcgccgac ggcgggcgaa tacggcctgc ctctgacggt cacgagacag
      301 acctgccgcg agaaggacgg cataagcggc ctcgcctcga cgccatcgtg cttcacactc
      361 gatatgggca tgaaggcaag gccgaggccc gccgcgacca tggccaggat ccagtcgtcg
      421 cgctcgcttt cataaactgt cggccactcc acctgctgct cgtcgaaaac atggtcgatg
      481 ctgtcgccga attcacagtt gatgcgcgaa agatagcact ggccgttgag cgacttgatg
      541 ggaacgacat tatgcgcaca aatcgagtga ccccgcggcg cgacggcgac gaagtcttcc
      601 tcgtaaagcg gaatgtagtg caagcggcta tccagcggct tgcccggcgt cgcgtagatt
      661 gcaacctcta gctcccccgc tagcagatca tcctccaatt gccgcgcggg cgcgtcggcg
      721 atcttgaggg ggacgccggg atggcgttcc agaaccgcct tgatgaaatc gatcagattg
      781 tcgggcgcaa tagtgcacat cagaccgagc cggagcggcg tcttcttcag gttcatgaac
      841 tcagcagctc gccgcttggc gttttgcgct tcattataca cttgctccag atagggcttc
      901 atcatgcgcc ccagctcgga cagatgagta ttcgagcgtt ctcgatgaaa aagcgggccg
      961 ccaagttcga gctcgagcat tttgatggcg cgggtaatcg agggctgaga aacgttgcac
     1021 cgttccgcag cccgggtgaa attcagctct tccgcgagga cgataaaata cttgatctcg
     1081 tgcatttcca tattaaaggc ccctgccaga tggaacttca gtgagtccca gtcatgttcg
     1141 cgctttcgag gcttcgtgca aaacaaatct cattcatagc tttcctgcat tgaatcaata
     1201 tccaatccgc atttaaagag ctgtctctcc tcgcctaaat aagctggagc agtgcaagac
     1261 tcttacgatc gccgcccgtc acgatccagt gtccttgacg tttcatcttt gacacggctc
     1321 ggcgcttgga tgaggttggg gacaacaaag ttgatttcac gagggttatc aaagatgccg
     1381 ggcagtgaaa tcggccagga tcgccggatt agggtgcatt cgcgctttgt gcgcgcgacc
     1441 cactggatca acgcagtcgc aatcctcatc atgatcggaa gcggctggcg catctacaat
     1501 aatatgccga tcttcagttg gctcacattc cccgaatggg cgacgctcgg cggcgatcct
     1561 gagatcacct acaagctcaa caaggacgta ggcttctcga acgcgcttct gtggcacttc
     1621 gcggccatgt ggctcttctt tgtgaatggc gttgtggcgc tgggcgtggg cctggccagc
     1681 ggccgattgc aaaggaagtg ggcgccgatc tcgttgtccg agttggtcca cgatatccgg
     1741 caggcgctca gtttcaacct cgctcatgat gacatcaccg tctacaacgc ggtgcagaag
     1801 ctcctctata gcggcgtaat cctggcgacg atcttaatgc tcgtatccgg cctcgccatc
     1861 tggaagccgg tgcagtttca agcgctgacg tggctcttct atgatttcca gggcgcaagg
     1921 ctcgcccact ttatcggtat ggccgcgatc gcgctgttcg tactggtgca cgtcatgctc
     1981 gccatcctcg ttccgaaaac aattaccgct atgaccaggg gctcgatccc tgctgaaggc
     2041 cccgcgggca tctcacctgt ccgggcggga gaatagccat ggccatcata cgcccgagac
     2101 gatccgctaa gcgcccgcaa ggcggcgtcg atcaatcggc cttgatcgag catcgcgagc
     2161 ttatcgccgg gatcgagagg cgcgcattct tgcgcggatc ggtaagcctc ggcgcgctga
     2221 cgctgctaac gggctgcgac atcagcaatg acagcgcctt gcaaggcgcg ctccgcgcca
     2281 tgtcgggctg gaacgaccgc gtgcaggctt ggcttttcga cccgaagaaa ctcgcgccca
     2341 cctattcgga cgccgacgtg ctcaatcccc cgcgctttaa tgccttctac ggcatcgacg
     2401 aggtgaagaa ggtcgatggc gcgacatggc gtctggaact cgcgggcgcc atcgagaaca
     2461 aacagccctg gaccaaggac gcgctttatg cgcttccgcg ggaaacgcag atcacgcggc
     2521 atatctgcgt cgaaggctgg gactatatcg gcaagtggtc cggtccaccg ctgcgcacgt
     2581 ttctcgaccg gattggcgcc gacaagactg cgaaatatgt cggcttcaag tgcagcgaca
     2641 attattgggg cagcatcgac atggcgacgg cgctgcatcc gcaaaccatt ctcgcgacgc
     2701 attacgcggg tgatcccctg ccggacgctt acggctttcc ggtgcgcctc aagacgccgc
     2761 tgaagctcgg cttcaagcag cccaaatgga tctacgccat ggaagtgacg aacgtttacc
     2821 ccggcggcta ctgggaagac ggcggctata actggttcag cggcctctaa agcaaaaaag
     2881 gcaggcgcaa agcaaagat
//
LOCUS       DATMGX010000688         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_388325, whole genome shotgun sequence.
ACCESSION   DATMGX010000688 DATMGX010000000
VERSION     DATMGX010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388325"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VNR65_11290"
     CDS             <1..1127
                     /locus_tag="VNR65_11290"
                     /inference="COORDINATES: protein motif:HMM:NF019944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HWJ19299.1"
                     /translation="RREPQAPSASVVIRTETFEAPSAAAIRQIVAAAVPGMRPDNVAV
                     LNTDGTILAAGGESAGATTGKMLDLEKQVSKEITENIRKTLTPMLGLGNFEASVSTRL
                     NLDKKETNETAFDPESKVERSTRTLKETSNSQTANTKWNVSVEQNIPNDQGAAKPSDQ
                     NRKSSERKEETNNFELSSKTTTTISDGYRIENIAVAVVVNRKPLLTSSGDTKPAAIDE
                     QIKSIEKLVASAAGLQPGRGDKATVVAVDFAAGTEFEPVASSSTFWDQIAGQSGNTLI
                     AAAIVLSTIIFVSFGLRPSLRLILENRKPALEAVGAEPRLAQGVAEPALEVDAAQIAQ
                     PTLAGPIVPKKPSPIRQVEKAIDENEEMAAEIVKQWIREG"
     gene            1129..1734
                     /locus_tag="VNR65_11295"
     CDS             1129..1734
                     /locus_tag="VNR65_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19300.1"
                     /translation="MAVVPLMLFLARRDGTHGNTASGGPEIPDIRAASEAWYQKGIAE
                     GMEKAREVSEAAIAQKEEECKSRLIQARRSWSQTQAALLAQLTTASIAGLKSEIEETV
                     ARILRPLVEKALVEDALSKFAIEIGKLLSDDDAIRLRISGPSDLVSQLGSLIPQNVPV
                     TIIEGDTPEVTVFANKTVIETRLHEWLECTGVDSHAAQEEG"
     gene            1712..>2180
                     /locus_tag="VNR65_11300"
     CDS             1712..>2180
                     /locus_tag="VNR65_11300"
                     /inference="COORDINATES: protein motif:HMM:NF025057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HWJ19301.1"
                     /translation="MPPRKKGNESTAPTIIIIKKKVHDSEDGHHGGTWKIAYADFMTA
                     MMAFFLVMWLVSTASKEQITRLASYFNPVKLSDRNPAKGVHDNEAGTPEEKAEAAKAE
                     KAEKKAEKPSKKSEAEKKPDAEKKPETEKHQSEDEQLFTNPFGVLTQLASQAEG"
BASE COUNT          575 a          623 c          605 g          377 t
ORIGIN      
        1 tccgccgcga gcctcaggct ccctcggcgt ccgtcgttat ccgcaccgaa acttttgaag
       61 cgccttccgc cgctgcaatc cgccagatcg tcgctgcagc ggtgccggga atgaggcccg
      121 acaacgtcgc cgtactcaac accgatggaa cgatccttgc cgctggcggc gaaagcgctg
      181 gcgcaacgac tggcaaaatg ctcgatctcg aaaagcaggt gagcaaggaa atcacggaga
      241 acatccggaa gacgcttact ccgatgctcg ggcttggtaa tttcgaagcc agcgtgtcga
      301 cgcgcctcaa cctcgacaag aaagagacga acgaaacggc cttcgatccg gaatccaagg
      361 tcgaacgttc cacgcggact ctcaaggaaa catccaactc tcagaccgcg aacacaaaat
      421 ggaatgtcag cgtcgagcag aacattccga acgatcaggg agcggcgaag ccctccgatc
      481 aaaaccgcaa atccagcgaa cgcaaagaag aaaccaacaa ctttgaactg agctctaaga
      541 ccaccaccac catcagcgac ggatacagga tcgaaaacat cgccgttgcc gttgtggtca
      601 accgcaagcc gctgctcacc tcttcaggag acaccaagcc agccgcgatc gacgagcaga
      661 tcaaatcgat cgagaagctt gtcgcctcag ccgccgggct acagcctggg cgcggcgaca
      721 aggcgacggt cgtggccgtc gatttcgccg ctggcaccga gtttgagccg gtcgcgagtt
      781 cgtccacgtt ctgggaccag attgctggtc agagcggcaa caccctcatc gcggctgcga
      841 tcgttctgtc gacaatcatc tttgtttcct ttggcctccg cccctctctg cgcctcattc
      901 tcgagaatag aaaacccgct ttggaggcag ttggggcaga gccacgactt gcacaaggcg
      961 tggcagagcc ggcgctagag gtggacgctg cgcagatcgc ccagccgacc ttggccgggc
     1021 ctattgttcc gaagaagcca tctccgatca gacaggtgga aaaggcgatc gatgagaacg
     1081 aggagatggc agccgagatt gtgaagcagt ggataaggga gggctgaaat ggcagtcgtt
     1141 cccttgatgc ttttcctcgc acgacgtgac gggacccacg gcaataccgc cagcggcggc
     1201 cccgagatcc ctgacatccg cgccgcatcg gaggcgtggt atcaaaaagg tatcgcggaa
     1261 gggatggaaa aggccagaga agtctctgaa gctgccatag cccagaagga agaggagtgc
     1321 aagtcacggc tcattcaggc cagaaggagc tggtcacaga cacaagcagc actccttgcc
     1381 cagctaacca ccgcctcaat cgctggcctg aaaagcgaga ttgaggaaac cgtcgcccgc
     1441 attctaaggc cccttgtcga aaaggccctc gtcgaggacg ctttatctaa atttgcaatc
     1501 gagatcggta agctcctttc agatgatgat gcgatacgct tgaggatctc cgggccttcc
     1561 gacctggttt cccaattggg cagtcttatt ccgcagaatg tccctgtaac tattatagag
     1621 ggagacaccc ctgaagtaac agtatttgcg aataagactg ttattgaaac tagattgcat
     1681 gaatggcttg agtgtacagg agtagatagc catgccgccc aggaagaagg gtaacgaatc
     1741 aaccgcgcca acaataatta tcatcaagaa gaaagttcac gattccgaag acggccatca
     1801 cggcggaact tggaaaatcg cctacgcaga tttcatgacg gccatgatgg cctttttcct
     1861 tgtgatgtgg ctcgtcagta ccgcctcaaa ggagcaaatc acaaggctcg cgagttattt
     1921 caaccccgtc aaactgagcg atcgcaatcc tgcaaaaggc gtgcacgaca acgaagccgg
     1981 cacgccggag gagaaggctg aagccgcgaa agccgagaaa gccgagaaga aggcggaaaa
     2041 gcccagcaag aagtccgagg cagagaaaaa gcccgacgcc gagaaaaagc ccgagaccga
     2101 gaagcatcag tctgaagacg aacagctctt caccaatccg ttcggtgtcc tcacccagtt
     2161 ggcgtcccag gcagaaggcg
//
LOCUS       DATMGX010000689         2071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_75514, whole genome shotgun sequence.
ACCESSION   DATMGX010000689 DATMGX010000000
VERSION     DATMGX010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75514"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VNR65_11305"
     CDS             complement(<1..284)
                     /locus_tag="VNR65_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HWJ19302.1"
                     /translation="MAASLPFGLAIGMALARRDFWGKGLVNGIVHLPLVMPPVVTGYL
                     LLLTFGRKGPIGAFLDQYFGIVFSFRWTGAALACAIMGFPLMVRAIRLSA"
     gene            complement(356..1165)
                     /gene="modA"
                     /locus_tag="VNR65_11310"
     CDS             complement(356..1165)
                     /gene="modA"
                     /locus_tag="VNR65_11310"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF007958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWJ19303.1"
                     /translation="MSIGTRLVASFSATLLASVLLVGQTASAQDGDANAPVIVFAAAS
                     LKNALDKVSANWEQESGQTVKTSYAASSTLAKQLEQDAPAQIFISADLDWMDYAAAKG
                     LIKKESRSDLLGNKLVLIAPKDSSASVDLKLGAVLAKALGDGRLAIGNVDSVPAGKYG
                     KAALGKLRLWDTVSNKLAQAESVRAALLLVSRGEAALGIVYQTDAAADPNVKIVATFP
                     ADSHPTIIYPVALTLKANAAAASFLDYIKSPKAAPAFEAQGFTVLNTEKRS"
     gene            1419..>2071
                     /locus_tag="VNR65_11315"
     CDS             1419..>2071
                     /locus_tag="VNR65_11315"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HWJ19304.1"
                     /translation="MIDTFNSGVSGLPEMEGLLLDDIHLLTSLLSETIREQEGVQTFE
                     TIETIRRLSTAFEHEADPEAGLELDRLLGWLKPHEAVQVARAFCYFSYLTNLAEDRHR
                     IRCTAASLNGNPGEEAQGSLDWTFKRLAEAGITPEQTCEALKGSLVSPVLTAHPTEVQ
                     RRSILDATSAIEHLLAARETLSNERDLKRNESLMRARIVQLWQTRLLRFAHLTVADE"
BASE COUNT          426 a          614 c          602 g          429 t
ORIGIN      
        1 tcggcggata gccttatggc ccgaaccatc agcgggaacc ccatgatggc gcaagcgaga
       61 gccgcgcccg tccaccggaa cgaaaagacg atgccgaaat actgatcgag aaaggccccg
      121 atagggcctt tcctgccgaa ggtgagaagg agcaaatagc cggtcacgac cggcggcatg
      181 accaggggga gatgcacgat cccgttcacg agcccctttc cccaaaagtc gcgccgcgct
      241 aacgccatgc cgatcgccaa gccgaacggc aggctcgccg ccatggcgac ggtcgcaata
      301 agaaggctca gcctgatggc agtccattcg tcagggctaa gagtccacca actgctcagg
      361 agcgtttctc cgtatttaga accgtaaagc cctgggcttc gaatgccggc gctgctttcg
      421 gagacttgat gtaatcgaga aacgatgccg ctgctgcgtt cgccttcagg gtcagcgcaa
      481 ccggatagat gattgtcggg tgcgagtcag ctgggaaagt cgcaacgatt ttcacattgg
      541 gatcggcggc ggcgtcggtc tggtagacga tgccaagcgc tgcctcaccg cgcgaaacga
      601 gaagaagtgc ggcgcgaacg ctttcggcct gggccagttt gttcgagacg gtgtcccaga
      661 gcctaagctt tcccagtgcg gctttgccat atttgcccgc aggaaccgaa tcgacattgc
      721 ctattgcaag ccggccgtcg ccgagggcct ttgccaggac cgcgcctagc ttgagatcga
      781 cgcttgcaga gctgtctttc ggagcaatca acacaagctt gttcccgaga aggtccgagc
      841 ggctttcctt cttgatcagt cccttggctg ccgcatagtc catccaatcg aggtctgcgg
      901 agatgaagat ttgcgcaggc gcatcctgct ccagctgctt tgcgagcgtt gaactcgcgg
      961 cgtagctcgt ctttaccgtt tggcccgact cttgctccca attcgctgaa actttgtcga
     1021 gcgcgttttt cagactggcg gctgcaaaaa cgatgacagg agcattggca tcgccgtctt
     1081 gcgctgaagc ggtttggcca acgagaagga cagaagccaa tagtgttgcg gagaaagacg
     1141 ccaccaagcg tgttcctatg ctcattcttg tacccctttt atcgatcacg gtgtatccgt
     1201 ctgtatatag ctcatgcagc actctgtcga tctcaatacc cagggctcgt gctttatgag
     1261 cgggcttcgc cccttgtgcg caggatttcg gcacatgcat ccggtcggcg aggcaggttc
     1321 gattcagggc agactgccac taagtacttg cgcgaagagc cgtttttcag ttctctcttc
     1381 gttggccggt tgtggccgcg ctctcctgcg gtgatacgat gattgatacg ttcaactccg
     1441 gcgttagcgg ccttccagaa atggaggggc tgctcctcga tgacatacac ctcctgactt
     1501 cgcttctcag cgaaacgatc cgtgaacagg aaggcgtgca aacctttgaa acgatcgaga
     1561 ccatccgtcg cctcagcacc gccttcgaac atgaagccga ccccgaagcc ggcctggaac
     1621 tcgaccggct tcttggctgg ctcaagccgc acgaagctgt gcaggtcgcg cgggcgttct
     1681 gctatttttc ttatcttacg aatcttgccg aggaccggca ccgcatccgc tgtaccgctg
     1741 caagcctgaa cgggaatccg ggcgaggagg cgcaaggcag tcttgactgg actttcaagc
     1801 gtctggccga ggctggaatt actccggaac agacatgcga agcgctcaag ggaagcctgg
     1861 tgtcgcccgt gctgacggcg catcccaccg aggtgcagcg ccgcagcatt ctcgacgcca
     1921 caagcgctat cgagcatctg ctcgccgctc gggagacctt gagcaacgag cgcgacctca
     1981 aacgcaacga gtccctgatg cgggccagga tcgtccagct ctggcaaacg cggcttttgc
     2041 ggttcgcgca tctcactgtc gcggacgaaa t
//
LOCUS       DATMGX010000690         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_2277,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000690 DATMGX010000000
VERSION     DATMGX010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2277"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VNR65_11320"
     CDS             <1..360
                     /locus_tag="VNR65_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWJ19305.1"
                     /translation="SAKDPVNFAGMVAADVLRGDMPLSHWGAVDGAFVLDVREPFELA
                     VENVPGAVNIPLGQLRARLKELPRDRDIHVICRSGQRAYMATRILRQNGFKAWDVSGG
                     MLSLAHNGVLVASADAA"
     gene            complement(412..660)
                     /locus_tag="VNR65_11325"
     CDS             complement(412..660)
                     /locus_tag="VNR65_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ19306.1"
                     /translation="MVVNDDDLVREAIRAVLEDDGRVVESLGSCEPFSRPMIRAKSAC
                     PLVDAYLPGMSGLELLEKLHHEGHSLPAIMITGKPMFP"
     gene            complement(723..1763)
                     /locus_tag="VNR65_11330"
     CDS             complement(723..1763)
                     /locus_tag="VNR65_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HWJ19307.1"
                     /translation="MIQPPYANIILWIGIGFCIAQTGIFSGLNLAIFSVSRLRLEVEA
                     TGGNGDAAKLLRLRANSNFTLATILWGNVATNVLLTLLSESVLAGVGAFVFSTVVITF
                     LGEIIPQAYFSRNAVRLAARFSPFLRFYQVILFVVAEPTALFLNLWLGPESIALLHER
                     DFHTLLLKHVEAGSTDVGQLEATGALNFLDLDDIVAVDEGEPVDPRSIIRLPIVSGWP
                     VLPAFERTPRDVFLRQLDASGRKWVIFVDDSGAPRIVLDAHHFLRDVLFNEVSVGPEP
                     YWHRPIVVTDMGTRLGDVIGRMKTKPEHAEDDVIDHDLILVWGKQKRIITGADLLGRL
                     LRGIATREVRQS"
     gene            complement(2062..>2433)
                     /locus_tag="VNR65_11335"
     CDS             complement(2062..>2433)
                     /locus_tag="VNR65_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19308.1"
                     /translation="ARVAGFKSECLRVQIGPAPQKATAWDDAGSIEAGLNEAAGSLAA
                     SYGRWSFAAHPLELAGWARPTMSESRLDRADFKLDRTNGNRDPKSSVLPFDNNNYVQV
                     TLTRRHGSRLEVAVGSWRTIR"
BASE COUNT          533 a          711 c          722 g          467 t
ORIGIN      
        1 agcgccaagg acccggtcaa tttcgccggc atggtggccg ccgacgtgct gcgcggcgac
       61 atgccgctca gccattgggg ggcggtcgac ggcgcattcg tccttgacgt gcgcgagcca
      121 ttcgaactcg cggtcgagaa cgttccagga gcggtcaaca tcccgctcgg gcaattgcga
      181 gcccgtctca aggagcttcc gcgcgaccgc gacatccatg tgatctgccg atccggacag
      241 cgcgcctaca tggcgacgcg catactgcgg cagaacggat tcaaggcctg ggacgtgtcg
      301 ggaggcatgc tttcgctcgc ccacaacggt gtgctcgtag cgtcggctga cgcggcgtag
      361 ccgcgttgcg gacgtccacg cggcgacggg ttcaacaaag gaaagaagcg atcatgggaa
      421 catcggcttg ccggtgatca ttatcgccgg caggctatgg ccttcatggt gcagcttctc
      481 gagcaattcg agcccgctca ttcccggcag ataggcgtcg acgagcgggc atgcggattt
      541 tgcccggatc ataggccttg agaaaggctc gcagcttcct aaggattcga cgacgcggcc
      601 gtcgtcttcg agcacggcgc gtatcgcctc gcgaacgagg tcgtcatcgt tgacgaccat
      661 gatcgcagct gcgcccgaag cgctcggggc ttcatgagag tgatgagggg gcaagggata
      721 tttcaactct gtctgacttc ccgcgttgca attccgcgca ggagccgccc caagagatcg
      781 gcgccagtga tgatgcgctt ttgctttccc cacaccagga tcaagtcgtg gtcgatcaca
      841 tcatcttcag catgctccgg cttcgtcttc atgcgcccga taacgtcgcc cagtcgcgtg
      901 cccatgtcgg tgacgacgat cgggcgatgc caatagggtt ccggaccgac cgacacttcg
      961 ttgaagagga cgtcgcgcag aaaatggtgg gcgtccagaa cgattcgcgg cgcccccgag
     1021 tcgtcaacga aaatcaccca ctttcttccc gaagcatcaa gttgccgtag gaacacatcc
     1081 ctcggcgtgc gctcgaacgc gggcaacacc ggccatccgc tcacaatagg aaggcggatg
     1141 atgctgcggg ggtcgacagg ctctccttcg tctaccgcga cgatatcgtc gagatcgaga
     1201 aagttcagcg cgccggtggc ttccagttgt ccgacatcgg tgcttccagc ttccacatgc
     1261 ttcaacaaaa gggtgtggaa gtcccgctcg tgaagcagtg cgatactttc cggcccaagc
     1321 cacaagttga ggaacagtgc cgtgggttct gcgaccacaa acagaattac ttggtagaat
     1381 ctgaggaagg gagaaaaccg ggcggcaagg cgaacggcat tccgcgaaaa ataggcctgt
     1441 ggaatgatct caccgagaaa cgtgatgacg acggtcgaga atacgaaggc gcccacgcca
     1501 gccaagaccg attcggataa taaggtcaga agcacgttgg tagcgacgtt accccaaaga
     1561 attgtcgcca atgtgaaatt agaatttgcg cgcagacgaa gcagcttggc ggcgtcacca
     1621 ttcccaccag tagcctcgac ctccaggcgc aacctactga cgctgaaaat tgccagattc
     1681 agtcccgaaa aaatgcctgt ctgcgcgatg cagaatccga tcccgatcca gagaatgata
     1741 ttcgcataag gcggttgaat catatgccga cattctcctg atggtcgacg tttaaacaac
     1801 aggaattaac cattgcactc gacggggcga tgtcaccggc gttgctgcag gctcatgggg
     1861 tgcggaaata gccgcgcggg ggaaaggcgt gtccgtcatc agtaccctgg agactggtcg
     1921 agggttatca cgaagggacc gttttctgga tgatcgttac cgcaaggagc gaagaggaga
     1981 caaaaatccg gggggcgaag caacctgcgg aagtcgcaat caaggatatc ggcgagcgcg
     2041 aagtgcgttt ggcctcgttg gttacctgat ggtccgccag ctcccaaccg caacttccag
     2101 ccgcgatccg tgacgacggg ttaacgtcac ctgtacgtaa ttgttgttat cgaaggggag
     2161 cacgctgctt ttcgggtcgc gatttccgtt cgtccggtcg agcttgaaat cagcgcgatc
     2221 aaggcggctt tccgacattg tcggccgcgc ccaccccgcc agctctaaag gatgcgctgc
     2281 gaacgaccaa cgcccatagc tcgcggctaa cgaccccgca gcttcgttca atccggcttc
     2341 aatagatccc gcatcatccc acgcagtagc cttttgcggc gcgggaccta tttgaaccct
     2401 caggcattcc gatttgaagc cggccacccg agc
//
LOCUS       DATMGX010000691         2373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_314821, whole genome shotgun sequence.
ACCESSION   DATMGX010000691 DATMGX010000000
VERSION     DATMGX010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314821"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..249
                     /locus_tag="VNR65_11340"
     CDS             25..249
                     /locus_tag="VNR65_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWJ19309.1"
                     /translation="MGDVGRMLIFVGGLLLVFGVVLILAGKVNLPIGRLPGDIVYRDK
                     NTTFYFPLVTSILLSVILSLVLYVVNRMRR"
     gene            426..1889
                     /gene="xseA"
                     /locus_tag="VNR65_11345"
     CDS             426..1889
                     /gene="xseA"
                     /locus_tag="VNR65_11345"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWJ19310.1"
                     /translation="MADTQQLGLAFQAPSRKIYPVRDLVAAVRTQVERAFTDIYVEGE
                     ISNYRPADSGHLYFTLKDGSAQLRVVMWRTQARLLRFRPENGLQIIARGRVTIYDERG
                     DLQFQAEYLEPKGAGALQIAFEQLKTKLAAEGLFDPSRKRPIPALPRRIGVVTSPRGA
                     AIQDILNILRRRHESVSVLIYPAQVQGESAAAEVSAGVRYFNRHGKVDVIVVARGGGS
                     FEDLFAFNDEALARTIAAAHVPVISAVGHETDFTICDFVADLRAPTPSAAAELVIGSK
                     QELAERLVSLRRRMAQAVNYKLLRAHNALSRLAQHAVFARMQDSIARRQQRVDDLVSR
                     MAQSQSQGLKNLSRRLEDVEAQLRRHDPRVRVGVMRRQLDGQTAALHSSVMRRLVGRS
                     NELGGLTVALGRASETILLRRRAHWERLHSSLEALSPKAILARGYALVFDDEGRLVKQ
                     AAQLKPGSRVRAQLGRGEFTAEVDQVKDEPANNLDNE"
BASE COUNT          452 a          725 c          719 g          477 t
ORIGIN      
        1 tgtgacctga tttaatctag agccatgggc gatgttggcc gcatgctgat ttttgttggt
       61 gggctgctgc tggtctttgg ggtggtcctc atcctcgccg gcaaagtaaa cctgcccatt
      121 ggacgcttgc cgggggacat cgtctatcgc gacaagaaca cgacgttcta tttccccctg
      181 gtcacctcca ttctgctgag cgtcattctg tcgctagtgc tgtatgtggt gaaccgcatg
      241 cggcggtaaa acaaccgtca gctctcagcc gtcagctttc agctaagcct caactccgaa
      301 agatcatcgg catgtcaaag cgccgacagc gagccagcga gtctgaaagc cgatggccga
      361 tagctgacgg ctgaaagctg agagctgacg gcttcagttg atacactgcc tccagccgct
      421 cttccatggc cgacacgcaa caactcgggc tggccttcca ggcgccctcg cgcaagatct
      481 atccggttcg cgatctggtc gccgcagtgc gtacccaggt agagcgcgcc ttcaccgaca
      541 tttacgtcga aggcgaaatc tccaactacc ggccagccga ttctggacat ctttacttca
      601 cgttgaagga tggctcggcg cagttgcggg tggtgatgtg gcggacgcag gcgagactgt
      661 tgcgctttcg gccggagaat ggcctgcaga ttatcgctcg cgggcgagtc accatttacg
      721 acgagcgcgg cgacctgcag tttcaggccg agtacctcga gcccaagggc gcgggcgcgc
      781 tccagattgc cttcgagcag ttgaagacca agctggctgc cgaagggctt ttcgatccca
      841 gccgcaagcg gcctatccca gccctgcctc ggcgcatcgg agtggtcacc tccccgcgtg
      901 gcgccgctat ccaggacatc ctgaacattc tgcgccgccg gcacgagtcc gtgagcgtgc
      961 tcatttatcc cgcgcaggtc cagggcgaga gcgccgccgc cgaagtaagt gcgggcgtcc
     1021 gctacttcaa tcgtcatggc aaggtggatg tgatcgtggt ggcgcgcggc ggaggttcgt
     1081 tcgaagacct cttcgctttc aacgatgaag ccctggcgcg cacgattgca gccgcgcacg
     1141 ttcccgtgat ctcggccgtc ggccacgaga ccgacttcac catctgcgat ttcgtcgccg
     1201 acctgcgcgc accgactccg tcggcagcgg cggaactggt cattgggtcg aagcaggaac
     1261 tggcggagag gctcgtttcc cttcgccgac gcatggcgca agcggtgaac tacaaactgt
     1321 tgcgcgccca caatgctctt tcgcgtctgg cccagcacgc cgtcttcgcg cgcatgcagg
     1381 acagcatcgc gcggcgccag cagcgggtcg acgatctggt gtctcgcatg gcgcaatcgc
     1441 aatcgcaggg gctgaagaac ctgtcgcgcc gcctcgagga tgtcgaggcc cagcttcgcc
     1501 gccatgatcc tcgggtgcgc gtaggcgtga tgcgccgcca gctcgatgga caaacggctg
     1561 cgctacattc ctcagtgatg cgacggttgg ttgggcgaag caacgaactc ggcgggctga
     1621 ccgtcgcttt gggccgggcg agcgagacca ttctgttgcg ccggcgggcc cactgggagc
     1681 gtttgcacag cagtttagag gcgctctcgc ccaaggcgat cctggcccgc ggttatgcgc
     1741 tcgtcttcga cgatgagggc cgcctggtga aacaagccgc ccagctcaag ccaggtagcc
     1801 gggtccgcgc ccagctcggt cgcggtgagt ttaccgcgga agttgatcaa gtgaaagatg
     1861 agccagcgaa taacctggac aatgaatagt gttggaacac gagattccca aacaagccag
     1921 caacccatcc agaatccaag caacgcgcgc aattctggaa atcatctgtc cgtgcgtccc
     1981 taaagcggtt ccaggaattg gtgcatccgc tcgtccgggt agtgcgggcc ttcaggcccg
     2041 cggacagggc aggaagagaa attgcttaag ccgctgaggt cgtcgacctc agcggcttaa
     2101 gcggggcgtt tgtccgcggc gcacgcggcg ctgaagcgcc gtactaaccc gtacttgcca
     2161 gcgtgcaccc attctgacgc tgctttagta ctcgctaatc actttccact atccactgat
     2221 cacttccggt agctctgcat tagaatgaag acactcagcg cggactctgg ccgcgagagg
     2281 aaaccgccaa aatgccaagc ctcagctaca ttccgcaaat gtcgatgact gcggtcttcg
     2341 atattctggt ggtggcgttt ctggtttacg agt
//
LOCUS       DATMGX010000692         2641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_228422, whole genome shotgun sequence.
ACCESSION   DATMGX010000692 DATMGX010000000
VERSION     DATMGX010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228422"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VNR65_11350"
     CDS             complement(<1..469)
                     /locus_tag="VNR65_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWJ19311.1"
                     /translation="MVPQDRLSQASPANINEKSIESLRENAEHLRLFVEQAPVGIAMF
                     GRDMRYLAASGRWLTDHALSEASVVGRSHYEVSPEIPQRWKDVHRRCLAGETVREEQD
                     PYKRADGRLLWLRWEVRPWRAAKGSIGGILIFTEDVTARVEMERALRESREDLD"
     gene            complement(674..970)
                     /locus_tag="VNR65_11355"
     CDS             complement(674..970)
                     /locus_tag="VNR65_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19312.1"
                     /translation="MSTLLHSYTVTNAIVEEMAALGEQPSKQAYDKPAAENAATAKAL
                     INSLMQDKFFLALFRSNDPSVRAAALDKLNEAHYQAYGDAPVGWPGGSFTPPRF"
     gene            1784..>2641
                     /locus_tag="VNR65_11360"
     CDS             1784..>2641
                     /locus_tag="VNR65_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWJ19313.1"
                     /translation="MHNAADVGHKDVAEFNKAEVNAEARTRFTPLHAAALRGHKDVAD
                     LLLAKGAEVNARANDEWTPLNIAALHGHKDVVALLLAKGAEVTAKSNLGQTPLHYAAS
                     AGDKDVVELLLANGAVVNARDTFHRTPLYDAAEKGHKDVAELLLASGAEVNAEANTRF
                     TPLHAAAAHGHKDVAALLLANGADVNAKSKHGQTPLELAARSDRYDVVELLRNHGDKV
                     SMIIDTTIHDAAKDGDLEKVRALLKGNPGLVSRHDDARNTPLHAAAFGGHGDVARFLL
                     GSEAEVNAEN"
BASE COUNT          546 a          760 c          782 g          553 t
ORIGIN      
        1 ggtccaggtc ctcgcggctt tcacgcagcg cgcgctccat ctcgacacgg gcggtcacgt
       61 cctcggtgaa gatcaggatc ccgcctatgc tccctttagc cgcacgccaa ggccgcacct
      121 cccatctgag ccataacaag cgcccatcag cgcgcttgta cgggtcctgc tcttcccgca
      181 cggtctcgcc ggcgagacag cggcgatgca catccttcca gcgctgcgga atctcagggg
      241 agacctcgta gtgcgagcgg ccaacgacgc tcgcttcgct aagtgcatga tcggtaagcc
      301 aacgtccgct ggcggcgaga tagcgcatgt cgcgaccgaa catagcgatg ccgaccggcg
      361 cctgctcgac gaacaagcga agatgttccg cgttctccct caaggactcg atcgactttt
      421 cattgatgtt ggctggactt gcctggctca gcctgtcctg aggcaccatt gctgtttcgt
      481 cgctccctct cctcctgctc ttgattggag acgatcctgc ggcagaagtg ttttttcagg
      541 caatgatttt gcattcatgg tcgatgctgg agttgacctg gatcaaaagc acgagagcga
      601 tctgaagcct gattggaaac agcgcgttcc ggttaggccc caggcgggct tcgggtcttc
      661 gccgactttc ggatcagaag cggggcggtg tgaaggagcc gccaggccac cccactggcg
      721 cgtcgccgta agcttgatag tgagcctcgt ttagtttatc gagggcagcg gcacgcacac
      781 tcggatcgtt actacgaaaa agagcaagga aaaacttatc ctgcataagg gaattgatta
      841 atgctttcgc cgtcgcagcg ttttctgccg ccggcttgtc gtaagcctgc tttgaaggct
      901 gttcccctag agctgccatc tcctcgacga ttgcattagt gacagtatag ctgtgtagca
      961 aagtggacat aatgctctcc ttattctaga aattgactgt ggctccgcta ccttttgccc
     1021 tcaccgtaac cgccgaaatc aaagctcccc cgggcgccta cccggatggc aaactgaatg
     1081 actagagccg ttaattggtt ccgctgaagt tgattgacgt tttgccgcag gctcatcctg
     1141 gcgggggcct tccagcccgt cggcgcatgg cgagcaacgc gggcaacgtc gcacctcgcc
     1201 attcccggat tgatctggca gacgcctttt ggggcatgaa cttgagaacg gctggaatat
     1261 tgccttgtag aaccgtgaat gttcagctct tgcccaagag cgtcttctcc ggatcattca
     1321 ggcggattga tggagggcga tggtgttgct gcggtcgcca taggccacga tctccttcgc
     1381 cggatcggcg ccaacttgtt tgtttgcggc ggcattcaca ccgaggaaat gccgctgggc
     1441 cgaacattgt gcgtctcgac atcccaagcg tcagggttgc tcagccgctg tcgaggcgct
     1501 caagcttgtt tgaatgggcg cgccatctcc cggacggggc cggtcaatac caaccccatc
     1561 gggattatgc actggcgtcg gcatcgtata atgcgtaaag atccagccct tcgctcccga
     1621 ggattagaaa cggaccaaga acatgcggct ctggaacaag ctattcggca tcaaaccgcc
     1681 agcctccttc gagtcgcgca gtggcggtta caccgccgca aacaactagt agacaaccaa
     1741 tggcggtctt gttttccgct aaaacaacct aaggaaaacg cctttgcaca acgcggcgga
     1801 tgtgggccac aaggatgtgg cggagttcaa caaggccgag gtgaatgccg aagccagaac
     1861 ccggtttacg cctttgcacg cggctgccct tcgcggccac aaggacgtgg cggatttgtt
     1921 gctggccaag ggggcggagg tcaatgccag ggccaacgat gagtggacgc ctttgaacat
     1981 tgcggcgctt catggccaca aggatgtggt ggcattgctg ctggccaaag gcgctgaggt
     2041 cactgccaag agcaacctcg gccagacgcc tttgcactat gcggcgagtg cgggcgataa
     2101 ggatgtggtg gaattgttgc tcgccaatgg ggctgtcgtc aacgccagag acaccttcca
     2161 caggacgcct ttgtacgacg cggcagagaa gggccacaag gacgtggcgg aattgctgct
     2221 cgccagcggg gctgaggtca atgccgaagc taacacccgg tttacgcctt tgcacgcggc
     2281 agcggctcac ggccacaagg atgtggcggc attgctgctt gccaacgggg ccgacgtcaa
     2341 tgccaagagc aagcacggcc agacgccttt ggagttagca gcccgatccg accgctatga
     2401 cgtggtggaa ttgttgcgta atcatggtga caaggtttcg atgattatcg acaccacgat
     2461 tcatgacgca gctaaggacg gtgacctgga aaaggtcagg gcgctgctca aaggcaatcc
     2521 tggccttgtt tcgcgccacg acgacgcaag aaacacgcct ttgcacgctg cggcttttgg
     2581 tggccacggc gatgtggccc gattcctgtt aggcagcgaa gcggaggtca acgccgagaa
     2641 c
//
LOCUS       DATMGX010000693         2291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_142827, whole genome shotgun sequence.
ACCESSION   DATMGX010000693 DATMGX010000000
VERSION     DATMGX010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142827"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VNR65_11365"
     CDS             <1..204
                     /locus_tag="VNR65_11365"
                     /inference="COORDINATES: protein motif:HMM:NF025438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndB"
                     /protein_id="HWJ19314.1"
                     /translation="QMPNWESYADKLSSIDWRKSGPLWDGNIIQGGKVMTQQGPVKGA
                     VAKVRAAIGLAGPTPAEAESIAA"
     gene            complement(323..685)
                     /gene="dksA"
                     /locus_tag="VNR65_11370"
     CDS             complement(323..685)
                     /gene="dksA"
                     /locus_tag="VNR65_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HWJ19315.1"
                     /translation="MNERQRAYFRKKLTAWRDELLRESKETLDNLQESAQHPDVTDRA
                     SYESDRGIELRARDRQRKLIAKIDAALERIEDGSYGFCEETGEPIGVKRLDARPVATL
                     SLEAQERHERRERIYRDD"
     gene            1026..2150
                     /locus_tag="VNR65_11375"
     CDS             1026..2150
                     /locus_tag="VNR65_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ19316.1"
                     /translation="MIVKLAIRNLLHDRARFLVTLIGVLFAVVLLAVQLGLFLGARKM
                     ISGMVAHTTGDIWIAAYGANSFEQAPILTGRERFLALSVPGVDSVNPLVVAFSGWRKA
                     DSGTVNVIVIGSEKGKGSLKPWNIVDGAAAPALHDGVIVDRTYAKALGVSGVGAVAEI
                     NGQRVRVEGLTDGIRSFTTSPYVFTSMNNAREILSAPADQATFYIVNLLPGADPAQVK
                     RELAAKLPDVSVYTKAEFLKLNLDYWLFGTGAGVALLGGALLGLVIGTVIVAQTLYSS
                     TKDHLTEFATLRALGSSSSYIHKVILVQAILSAIIGFTIGIAVSLVVAQFSEVTPMPI
                     VLTPLLALLLLGATVAMCAISALSSIYKVTKIDPATVFAR"
BASE COUNT          442 a          666 c          655 g          528 t
ORIGIN      
        1 cagatgccta actgggaaag ctacgcggat aagctgagtt caattgattg gcgcaaatcc
       61 ggcccgctgt gggatggcaa cattatccag ggcggcaagg tgatgaccca acagggaccg
      121 gtaaaagggg cggtcgcgaa ggttcgtgcc gcgatcggcc ttgccggacc tactccggcc
      181 gaagccgaaa gtattgccgc ttaagcgact ctaaagcgtc cccgcaaaga cgtttggccc
      241 cctgctccgc gggggccata ttttttggcg cccacgccaa ccggggattg aaagcggtga
      301 accccgagta aaggatgctt cgtcaatcgt cgcgatagat gcgctcgcgg cgctcatgcc
      361 gctcctgggc ttccaggctc aaagtcgcca ccggcctcgc gtcgaggcgc ttcacgccga
      421 tcggctcgcc tgtctcttcg caaaaaccgt aggagccgtc ctcgatgcgc tccagagccg
      481 catctatttt tgcgatcagt ttgcgctgcc ggtcgcgggc gcgaagttcg ataccccgat
      541 cggactcata gctcgcccgg tccgtgacgt cggggtgctg cgcagactcc tgaaggttgt
      601 ccagagtctc cttgctttct cgaaggagct cgtcccgcca ggcggtaagc ttttttctaa
      661 agtaagcgcg ctggcgctca ttcataaaag gttcgtcttc acacggctta taatctggag
      721 gcagatctag gccgcccatg tcgcccccta ttcgcacgaa cacccttact gatcatagcg
      781 agaacggatc aacacttaga aatctccatc gcactgactg cagcgttatg accttcaacg
      841 ccttccaagt caagaaggtg ccatcgacgc cccctccccg gctttgaccg gttgccgaca
      901 cggttttttg ttcaatgatt gaccgaaatt aaatgttagg cataacattg ccacaaaggc
      961 gctcttattg cgccgccctc gcgagtcgcc catctcccgc ttaacctggg actttgtcag
     1021 taccgatgat tgttaaactc gcgatacgca acctgttgca cgatcgtgca cgctttcttg
     1081 taaccctgat cggcgtcctt tttgccgtgg ttctgctcgc cgtgcagctt gggcttttcc
     1141 tcggtgcgcg gaagatgatc tccggcatgg ttgcgcacac aacgggtgac atatggatcg
     1201 cggcttatgg tgcgaacagc ttcgaacagg ctccgatcct gaccggacgt gaacgttttt
     1261 tggcgctgtc ggtgcctggc gtcgactccg tgaaccccct cgttgtggct ttttcgggct
     1321 ggcgtaaggc tgactctggc accgtgaacg tcattgttat cggctcggag aaggggaagg
     1381 gaagccttaa gccgtggaac atcgtcgacg gggccgcagc acccgccttg cacgacggcg
     1441 tgatcgtcga ccggacctat gccaaggccc ttggcgtttc gggcgtaggc gcagtggcgg
     1501 aaatcaacgg acagcgggtg cgcgtggaag gcctgacgga cggcattcgg tcgttcacca
     1561 catctcccta tgtttttaca agcatgaaca atgcgcgaga aattctttcg gcgccggccg
     1621 atcaagcgac cttttatatt gttaaccttc tgccgggcgc cgatccagcc caggtgaagc
     1681 gggagttggc ggcaaaactg ccggatgtgt cggtctacac caaggcggaa ttcttgaaac
     1741 tcaatctcga ttattggctg tttggtacgg gtgccggcgt tgctctcctc ggcggcgcac
     1801 ttctgggcct tgtgatcgga acggttatcg tcgctcaaac gctttattcg agcaccaaag
     1861 accacctcac cgaattcgcc acgttgagag cccttggctc atcctcctcc tacattcaca
     1921 aggtgatact cgtgcaggcg atcttaagcg ccatcatcgg ttttactatt ggcattgcgg
     1981 tttcgctggt ggtggcccag ttcagcgagg tcacgccgat gcccattgtg ttgacgccgc
     2041 tcctggcgtt gctgcttttg ggggcaacgg tggcgatgtg cgccatttcg gcgctctcat
     2101 ccatatacaa ggtgacgaag atcgaccctg cgacggtgtt tgcccgatga cagcgggccc
     2161 tattcttgaa gcggaccaca tcgtcaaaga acttggcgaa ggcgacggca aggtcctcgc
     2221 gctgaaaggc gtcagtttgt ccctttaccc gggcgagttt acattgctca tgggaccgtc
     2281 agggagcgga a
//
LOCUS       DATMGX010000694         2890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_165566, whole genome shotgun sequence.
ACCESSION   DATMGX010000694 DATMGX010000000
VERSION     DATMGX010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165566"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VNR65_11380"
     CDS             complement(<1..362)
                     /locus_tag="VNR65_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWJ19317.1"
                     /translation="MVAIAKTSWIRVWDPLVRFGHWALVIAFAVAYLSGEEEGETSEL
                     HEWAGYIVGGIVVWRVVWGLTGPQRARFSDFVTGPMESLRYMVNLMTGRAKRYAGHSP
                     AGGAMVVALLISLALTVVT"
     gene            736..2178
                     /locus_tag="VNR65_11385"
     CDS             736..2178
                     /locus_tag="VNR65_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWJ19318.1"
                     /translation="MTGQDLAETDEHAASAALKERVALSSTAASAGITIAKLVAGLLS
                     GSLALLSEAAHALVDTAATIMTYLAIRTANKPADEGHQYGHGKFESMAALAETIILFI
                     LATVVVTQAWERLKTGGGEFEPTVLAFGVLIVSIIVDITRVYTLRRVARETGSQALAA
                     DAIHFASDMVGSVLVLLGLGAAALGFKYGDALAAMGVAGFIAIAGWRLGRQTVDTLLD
                     KAPAGVSDEIRAAVSKVPGVVEIETLRLRPGGTHLFGDVSVVVSRTLPLDQVAQVKRR
                     ILAAVKTAVPNIALSVDVIPKALDDETVLERVLLTAAQQRVPIHGVMVQNLDSGLSVS
                     LHIEVDGRMSIGAAHAKASKLESAIRAELGPEVEVETHIEPLMVKHLEGHDAPVGVRE
                     TVSAALMASARDIDGLVEIHDVRVRETPAGLVVIYHCHFPADQSVAAVHNAVDEVERR
                     MRIVRPDIVRIIGHAEPTRKSEAKAHAAHP"
     gene            complement(2303..2797)
                     /locus_tag="VNR65_11390"
     CDS             complement(2303..2797)
                     /locus_tag="VNR65_11390"
                     /inference="COORDINATES: protein motif:HMM:NF021165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HWJ19319.1"
                     /translation="MKTRLTGLSFAASVAIASACWAPSALADRDRPTIIADATVTKEC
                     GSCHMAFPAAMLPVRSWTAIMEGLSSHFGENAALDAAKTKEIQDWLTAHAGDARNSNG
                     HYLRGLAKSETPLRITETPYWVREHRKEVRESEYANPKVKSKANCVACHKDATKGNFE
                     GDDD"
BASE COUNT          555 a          873 c          871 g          591 t
ORIGIN      
        1 gtcacgacgg tcagcgcaag tgaaataaga agagccacca ccatcgctcc gccggctgga
       61 ctgtggccgg cataccgttt tgcacggccg gtcatgaggt tgaccatgta gcgcaacgat
      121 tccatcggac cggtcacaaa atcgctgaac cgggcacgtt gcggtcctgt cagaccccac
      181 acaacgcgcc aaacaacgat acctccaacg atgtagccgg cccactcgtg cagttcgctg
      241 gtctcgcctt cctcttcgcc gctgagatag gcgacggcaa aggcgataac gagcgcccag
      301 tgcccgaaac gcaccagcgg atcccaaact ctgatccatg aagtcttggc aatcgccacc
      361 atcgttggct ctccagctgc gattttctga gcggcccagt tacttgagcc gctgcgttat
      421 atcgggcatc ttgacgtccc cttcctgtca actcgctgac aacggcgctc acgctgccga
      481 cagggataat tcgttaatca ggcccgcgcg agcagagcag agtaatgcga gcaagcgtcg
      541 tacgtgcctc ttgggccaat ggcttcgccc cattcctaag ttttcaagtg ctgcttgatg
      601 atgataaatc ggttgccggg accattcctg caagggaaac cggcgctcgc aaaaccaacg
      661 gcacaacgtg ggagtcgtct ccgcttcatg cggcactgat agtatggcaa aacagtcccg
      721 gcaaaggagt gcgttatgac agggcaggat ctcgccgaaa ccgatgaaca tgccgctagc
      781 gccgcgctaa aggaacgtgt ggcgctgtcg tcgacggctg ccagtgcggg tatcaccatc
      841 gccaagctgg tggcgggctt gctctccggc tcgttggcct tgctctcgga agccgcccac
      901 gccctggtcg atacagctgc cacgatcatg acctatctgg ctatccgaac cgcgaacaag
      961 cctgcggatg aggggcacca gtacggccac ggcaaattcg agagcatggc ggcccttgcc
     1021 gagacgatca tccttttcat cctggcaacc gtggtggtca cgcaagcatg ggagcgcctc
     1081 aagaccggcg gcggagaatt cgagccgacc gtcctcgctt tcggcgtgct gatcgtctcc
     1141 attattgtcg atatcaccag ggtctacacc cttcgccggg tggctcgcga gaccggcagc
     1201 caggccctgg cggccgatgc catccacttc gcttccgaca tggtgggctc ggtcctggtt
     1261 cttcttggtc tgggtgcggc ggcgctgggg ttcaaatatg gcgacgccct ggccgccatg
     1321 ggggttgcgg ggttcatagc catcgcaggc tggcggctgg ggcgacagac cgtcgatacg
     1381 ttgcttgaca aggctcctgc cggggtcagc gacgaaatcc gagccgccgt atcgaaggtt
     1441 ccaggagtcg tcgaaatcga gactctgagg ctccgtcctg gcggcactca tctcttcggg
     1501 gatgtttccg tcgttgtgtc gcggaccttg ccgctcgatc aggtcgcgca ggtgaaacgc
     1561 cgtatccttg ccgcggttaa gacggcggtc ccgaatatcg cattgagcgt agacgtcatc
     1621 ccaaaggcgc tcgacgacga aaccgtgctg gagcgggtgc tgctgacagc ggcccaacag
     1681 cgtgtgccga tccacggggt gatggtccag aacctcgata gcggtctgtc ggtcagtctg
     1741 cacatcgagg ttgacggccg gatgagcatc ggcgccgctc atgccaaggc atccaagctc
     1801 gaaagcgcga tccgggcaga gcttggtccc gaagtcgagg ttgaaactca tatcgagccg
     1861 ctgatggtga agcatctgga gggacacgat gcaccggtgg gggtcaggga aacggtttca
     1921 gccgcgctga tggcttcggc aagggatata gacgggctcg ttgagattca cgatgtgcgg
     1981 gtccgggaaa caccggcagg ccttgtggtc atttaccact gccattttcc ggccgaccag
     2041 agcgtggcgg ctgtccataa cgcagttgat gaggtggagc ggcgaatgag aatcgtccgc
     2101 ccggatatag tccggatcat cgggcatgcc gagccaacgc gaaagtccga agcaaaagcc
     2161 cacgccgcgc atccgtagtg tctggacttt ctctgccacg taagcggtag acatgggaga
     2221 gctatcaccc tcgctccccg agggttacca acctcggaga gccagaggta atcgctttct
     2281 tgccgccggc gccgccgaaa gatcaatcgt cgtcgccctc aaagttcccc ttggtggcat
     2341 ccttgtggca ggccacgcaa ttcgccttgc tcttgacctt gggattggcg tactcggact
     2401 cgcgcacctc tttcctgtgc tcgcggaccc aataaggcgt ttccgtgatc ctcagaggcg
     2461 tttcgctctt tgcgaggccg cgcagataat gaccattgga gttgcgcgcg tcgcccgcgt
     2521 gcgccgtcag ccagtcctgg atttccttcg tcttggcggc gtccaatgcg gcattctcgc
     2581 caaagtggct ggataggccc tccatgatgg cggtccagga cctcacgggg agcattgccg
     2641 cggggaaggc catatggcat gagccgcatt ctttcgtcac cgtggcgtcg gctatgatcg
     2701 tgggcctgtc tctgtctgca agggctgaag gcgcccagca tgcgcttgcg atagccacgc
     2761 ttgccgcaaa cgacagtccg gtcagtctcg ttttcatttt caatccctct tccttgagcc
     2821 gctactggca atcgttgccg gagcttattg attaaaatgg ctccatcctc gctcgaaccg
     2881 ggtgagcaag
//
LOCUS       DATMGX010000695         2446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_383453, whole genome shotgun sequence.
ACCESSION   DATMGX010000695 DATMGX010000000
VERSION     DATMGX010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383453"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VNR65_11395"
     CDS             <1..721
                     /locus_tag="VNR65_11395"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HWJ19320.1"
                     /translation="RHYKTLASCLDKLKQTSGSSDLSRHPVPSYSNRIAAVPYFLGAE
                     QFANLQREVEALSGAERSYVPSHKKGATIAYETLIEHAPGVTAFYHSEAMAATISRMT
                     GVAVRPTPLHDQNSLSILIYERSGDHIGWHYDHNFYRGRHFTVLLPVVNHGNAADGHS
                     HARLKAMIGDAEAELQTPPNTLIVFEGARIMHKVTPLAAGERRVLISMTYCVDARANF
                     AQAIARRFKDTAFFGVRALWT"
     gene            892..2193
                     /locus_tag="VNR65_11400"
     CDS             892..2193
                     /locus_tag="VNR65_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWJ19321.1"
                     /translation="MAKKTSGEEKKTVVEKLNYPKCVEDGCWAPIPVPPPEGALMLKL
                     KDVDADLSSRIKDDEVMSFHLTGCTGHYGNDAPGQAVADAMAAQAENARLGGGSDAAK
                     KAAFFFHLGDIIYKDEDKKNPERADMQKLFNEQFYETYKHYPREIFAIPGNHDGKIKD
                     NEGKSAIDHFMENFCTSDRHLSNDDLNSSPRKTMIQPYPYWVFRTPLAYFVCLYANDI
                     NGGQLDDPESDATPQFNWLVDTLKAIRKEDGDRAVFLAIHYPPFSGGSNFPERGNPNL
                     GPTPHNRVLKPLAKLLREAFEASKLYPDAVFSAHAHLYQRLTYFCADGRQIPYLIVGS
                     GGHAPVEALAERCDGSFDAPPQLPVLSVAPAGYVFPAGESAQMVQYNDKEHGFLRMTL
                     DLKCRTLLGEYFAAYNAASGSTGVPALRDSFTLDLSKHRVS"
     gene            complement(2367..2443)
                     /locus_tag="VNR65_11405"
     tRNA            complement(2367..2443)
                     /locus_tag="VNR65_11405"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2407..2409),aa:Pro,seq:tgg)
BASE COUNT          523 a          733 c          704 g          486 t
ORIGIN      
        1 gcgacattat aagacgctgg caagctgtct ggacaagctg aaacagactt cgggtagctc
       61 ggatctttcg cggcacccgg tgccatccta cagcaaccgg atcgcggcag ttccctattt
      121 ccttggcgcc gagcagtttg cgaacctgca gcgcgaagtc gaggcgcttt cgggagcgga
      181 gcgcagctat gtgcccagcc acaagaaggg cgccaccatt gcctacgaaa ccctcatcga
      241 gcatgcacct ggcgtgacgg cgttttacca ttccgaagcc atggcggcca ccatttcgcg
      301 catgaccggc gttgcggtcc ggccgacccc gctgcacgat cagaattccc tttcgatcct
      361 gatctacgag cgttcgggcg accacatcgg ctggcattac gatcacaatt tctatcgcgg
      421 ccgtcacttc acggtgctgc tgccagtggt gaatcacggc aacgcggcgg acgggcattc
      481 gcatgcacgg cttaaggcca tgatcggcga tgccgaggcc gagctgcaaa caccgccgaa
      541 cacgctcatc gtattcgagg gtgccaggat tatgcacaag gtcacgccgc tggctgcggg
      601 tgagcggcgg gttctgatta gcatgacgta ctgcgttgac gcccgggcga acttcgctca
      661 ggcaattgcc cggcgattca aggacaccgc ctttttcggg gtgcgcgcgt tgtggactta
      721 aattgggagc gtaagccgga ttgccggtgg gttcccgaag atttcccgga acgattcctg
      781 cgcccgcaac cctataggca gggctgcggt tatggatgtt tgggaacttc gtcgccggca
      841 gccctcagcg tgcgaaggtc atgaaaggca ccgatgggag cgggaaacag aatggcaaag
      901 aaaacctcag gcgaagaaaa gaaaaccgta gtcgaaaagc tgaattatcc aaaatgcgtg
      961 gaggatggct gctgggcgcc gatccccgtc cctccgcccg agggtgcgct catgctcaag
     1021 ctcaaggacg tggacgcaga cctctcaagc cgcatcaaag atgatgaggt gatgtccttc
     1081 cacctcaccg gctgcacggg ccattatggc aatgacgccc cgggccaagc cgtcgccgac
     1141 gcaatggccg cgcaggctga aaatgcgcgt ttaggcggtg gaagcgacgc cgcgaagaag
     1201 gcggcatttt tctttcatct gggcgacatc atttacaagg atgaggacaa gaaaaatccc
     1261 gaacgagcgg acatgcaaaa gctgtttaat gaacagttct atgagaccta caaacattac
     1321 cctcgagaaa ttttcgccat cccaggaaat cacgacggca aaatcaaaga caatgaagga
     1381 aaatccgcca tcgatcattt catggagaat ttttgtacct cggatcggca tctatcgaat
     1441 gatgacctga acagtagccc gcgtaaaacg atgatccaac cctatcctta ttgggtcttc
     1501 cggacgccgc tcgcctattt tgtttgcctt tatgccaacg acatcaatgg ggggcagctc
     1561 gacgatcccg aaagcgacgc gacgccgcaa ttcaactggc tcgtcgacac gctgaaggcg
     1621 atccggaagg aagacggcga ccgcgcggtt ttcctcgcga tccactatcc gcccttttcg
     1681 ggcgggagca atttccccga gcgcggcaat cccaatctcg gccccacccc gcacaatcgc
     1741 gtgctcaagc cgcttgcaaa actcctgcgg gaggcgttcg aggcgagcaa gctctacccg
     1801 gacgccgttt tctccgccca tgcgcatctc taccagaggc tgacctattt ttgcgccgat
     1861 gggcggcaga tcccctatct catcgttggc agcgggggcc acgcgcctgt tgaagccctg
     1921 gcggagcggt gcgatggcag ctttgacgcc ccgcctcagc ttcccgtgct ctccgtcgcg
     1981 cctgccggat acgtctttcc cgcaggcgaa agcgcccaga tggtgcagta caacgataag
     2041 gagcacgggt tcctgcggat gacgctagac ctcaaatgcc gcacgctctt gggggaatat
     2101 ttcgcagcct ataacgcagc cagcggctca accggtgtcc ccgcgttgcg cgacagcttc
     2161 acgctcgacc tgagcaagca ccgcgtttcc tgattgcgcg cgaaggtgag ggggagatcg
     2221 gccgcttatg tggaggcttt cggactccgg ccaaacttcg ggattgcagg cgtagccggc
     2281 gcagttacga cgcccgcttc cgttccgctg aggctttctt gcggaactca gactttgaaa
     2341 tttctggctt aagtctttgc ttttattggt cggggcggag agattcgaac tcccgaccct
     2401 ctgctcccaa agcagatgcg ctaccaggct gcgctacgcc ccgact
//
LOCUS       DATMGX010000696         2117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_444895, whole genome shotgun sequence.
ACCESSION   DATMGX010000696 DATMGX010000000
VERSION     DATMGX010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444895"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..720
                     /locus_tag="VNR65_11410"
     CDS             352..720
                     /locus_tag="VNR65_11410"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ19322.1"
                     /translation="MVEATSITSGTLIAADKVKGTNVYNLAGDKLGSVDDIMIDKVTG
                     RAIYAVMSFGGFLGMGDQHHPLPWATLKYDTQKGGYVVNLDKKQLEGAPNYDRKSEFK
                     WTPEYGRKVDAYYKAPSYWT"
     gene            805..978
                     /locus_tag="VNR65_11415"
     CDS             805..978
                     /locus_tag="VNR65_11415"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWJ19323.1"
                     /translation="MDKDRVVGSEKQVKGAVKQAVGKAVGDAKLEAEGNADKKEGKVQ
                     NAIGGLKDTLKGE"
     gene            1442..1846
                     /locus_tag="VNR65_11420"
     CDS             1442..1846
                     /locus_tag="VNR65_11420"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ19324.1"
                     /translation="MRLYLLAAATFLAVVQFASAQAPSTAVPVGPSTERFIRLGDTAQ
                     LSSNIVGLDVYNNKDKIGDIKDIAMDNSGVRAYILSVGGFLGMGAHYVAVVPREINIG
                     YDAGDKKWHANMTATKAELLAAPEFKYEGRWQ"
BASE COUNT          478 a          544 c          626 g          469 t
ORIGIN      
        1 tcgacggcgg ccaagcgccg attcttggac tttttcaaca ttaccgaccc aaagcggtcc
       61 aacggcacgt gagatcacac ctggcgtccg tcgccgcgcg ccagcgcgag gtcctcgaac
      121 agctgggggg cacgcggtgg caggggacag atgggattca cctcacatcg ccaagcgttc
      181 cgtgtctgga gatacggctt gaagcgacat ttcgtcggcg cgattccaat cttggcgcgc
      241 tcttgattct gatcatcgcg cgtcttggtt tttccgttat tatttcattt gggttcgggc
      301 cggtcgaagt cgaaggcatc ttacccgtcg cttttgcaat ggagtttgac tatggtcgaa
      361 gccacttcga ttacctctgg aaccctcatc gcggccgaca aggtgaaggg caccaatgtt
      421 tataatctgg ccggggacaa gctcggtagc gtcgatgaca tcatgatcga caaggtcacc
      481 ggtcgcgcga tctatgcggt gatgtcgttc ggcggctttc tcgggatggg cgaccaacac
      541 catccgctgc cgtgggcaac cctgaagtac gatacgcaga agggcggcta cgtggtcaat
      601 ctggacaaga agcagctgga aggcgcgccg aactacgatc gcaagtcgga gttcaaatgg
      661 acacccgagt acggccgcaa ggtcgacgcc tactacaagg caccaagcta ttggacgtaa
      721 gctgctttgg tgcctgaacg cggcaaacgt ccgttgccgt cgaatcatca gaaatcgctc
      781 gtagatatat tggaggaaga aatcatggac aaggatcggg tcgtcggatc ggagaagcaa
      841 gtcaaaggcg cagtcaagca ggcggtcggc aaggcggtcg gtgacgccaa gttggaggcg
      901 gagggcaacg ccgacaagaa agagggcaag gttcagaacg ccatcggcgg tctcaaagac
      961 acgctcaaag gggaataggc gttgcggctg cgtgcttcgt tccttccgat cttggacatc
     1021 ctgccgggtc gaaggccaag gaaacggctg acgcagcccg gaaagtaggg attaccactt
     1081 ctcttctcat ggtcctgtct ctgatagtcg gcgcattcgt cgcgagcacc gcagcggcct
     1141 taggtggccg ccggcgtgat gaagtctgac agctactccc tcccgtcaat gtaactgtgc
     1201 ggggtcgtcc ggaatcggac gaccctttct tgtgaggttg ctcgttggct caggaacgag
     1261 gcatcgcaat ttttggtcct tgatatcgcg caaggacatt cggatcgttg ctgggtatta
     1321 ttagtttatg agccaaagcg tcacacttgg actcaaaagc tcagttcttc gaggtgtcct
     1381 cataggattt atcctcaatg cgatggttac acttggatgt gacagttcac ggagattgaa
     1441 tatgcgactt taccttctcg cggcagcgac gtttctcgct gttgttcagt tcgcttccgc
     1501 gcaggcacct tcgaccgctg tgccggttgg accgagcact gagcggttca tcaggctcgg
     1561 cgatacagcg cagctcagtt ccaatatcgt tgggctggat gtatacaaca acaaggataa
     1621 gatcggcgat ataaaagata tcgccatgga taattccggc gttcgagcat acatcctttc
     1681 agtcggcgga tttctgggca tgggcgcgca ctatgttgca gtcgttcccc gggagatcaa
     1741 catcggctat gatgccggag acaagaagtg gcacgcgaac atgactgcca cgaaggccga
     1801 actactggct gcgccggagt tcaaatacga gggccgttgg cagtaaagca aaagctgagt
     1861 cactcaacat ctacatcgac gaaaggccca gccttaccgc tgggcttttt tatactaaag
     1921 agtggttgtc cgcgatctcg tgagcttagt tcatatagca cccagggctc tcggagcgga
     1981 gccgaaaagt ggatgaacgg cttgaggggt tgcggcgtag cgtccgaagt cgcgagccaa
     2041 tcggcgattg aggctgagcc aggataagga gattcaaaga atgtcaattt ggaccatcat
     2101 tcttatcatc taagtct
//
LOCUS       DATMGX010000697         3774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_206872, whole genome shotgun sequence.
ACCESSION   DATMGX010000697 DATMGX010000000
VERSION     DATMGX010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206872"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /gene="tal"
                     /locus_tag="VNR65_11425"
     CDS             complement(<1..1233)
                     /gene="tal"
                     /locus_tag="VNR65_11425"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HWJ19325.1"
                     /translation="MSTNPLLQLHALGQSIWLDQMSRSLLTTGGLKRLIDEDGLRGVT
                     SNPTIFQKAISGSQDYAEQLEKLARGGSNVSQIYEEIVLQDIGNGSDVLRGVYDANDG
                     ADGFISLEVSPLLANDTKATIEEAKKLFAHLNRPNVMIKVPGTPAGLPAVEELLFSGL
                     NINITLIFAVDVYEQVAEAYIKGLERRVAAGLPVDRIASVASFFVSRIDGMVDKQLGQ
                     LAEKATDDQQKQRINALMGKAAIANAKVAYESFKRIFGSDRFAKLKEKGARVQRPLWA
                     STSTKNPAYPDTMYLDTLIGADTVNTVPPATLDAFRDHGTAKPTLDAGIEEAHRVFDE
                     LKAVGVDMTAVTTKLTEEGVASFSESFDELFEVIEARRVEVMRSIIARHGAALGKYQG
                     DFESALKSLDKQKAVSRMW"
     gene            complement(1230..1703)
                     /gene="rpiB"
                     /locus_tag="VNR65_11430"
     CDS             complement(1230..1703)
                     /gene="rpiB"
                     /locus_tag="VNR65_11430"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HWJ19326.1"
                     /translation="MTMKIAVGADHAGVPLNETVITELKRLGHEVVDCGTHDPSKPDD
                     YPDYAEAVGRAVLEGKAERAVLICGSGVGASVAANKLPGIRAALCHDCYSAHQGVEHD
                     NMNVLCLGSRVIGPELALDLVRAFLGAKFSGEQRHRRRLAKITALEEQDLRGLIK"
     gene            complement(1719..>3774)
                     /gene="tkt"
                     /locus_tag="VNR65_11435"
     CDS             complement(1719..>3774)
                     /gene="tkt"
                     /locus_tag="VNR65_11435"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWJ19327.1"
                     /translation="SIAVGPKDGFDNETQEQCELNRLCINTIRTLSMDAVQKANSGHP
                     GTPMALAPLAFTLWNRYLRHNPNNPHWPSRDRFILSNGHACMLLYSMLYLTGYDLSLD
                     DIKQFRQWGSKTPGHPEYGVVPGAEATTGPLGQGVSNSVGMAIAQRWLASQFNKDGHD
                     LFDYRVYAFCGDGDLMEGVSNEAASVAGHLGLSNLIWFYDNNHITIEGNTSLAFSDDV
                     GTRFLGWHWNVQQVSDVNDLPEVDRAIQTALKETERPSLIIVDTHIAYGSPHKQDTKE
                     AHGEPLGEEEVRLTKMFYGWPPDAQFLVPDEVKAYMGKAVERGAQGEKDWDGRYNAWA
                     KEFPDLAKTCQQMLKQELPDGWDKDIPSFPADAKGLATRDSSGKVLNAVAKNVPWLLG
                     GAADLDPSTKTKLSGTTSFEKGSYAGRNFHFGIREHAMGSILNGMALSKLRPFGATFF
                     VFSDYMRPVLRLAAMMDQPVIWIYTHDSFCLGEDGPTHQPIEHLMSLRAIPRLLLFRP
                     ADANEVAETWRYIMPLKTQPVALVLTRQAVPTFDRTKYASAAGLAKGAYIMADSGGTP
                     DIILMGTGSEVQLCVGAYEKLTANGVKARVVSMPCWRLFEEQSDEYKRQVLPPEVRAR
                     VAVEAGTDFGWKEYIGNDGYVVARTDFGESAPYKDLLKHFGFTVDDVVAKAQEVMKKI
                     KP"
BASE COUNT          713 a         1113 c         1124 g          824 t
ORIGIN      
        1 ccacatgcgg gagacagcct tctgcttgtc gaggctcttg agggcgcttt cgaaatcgcc
       61 ctgatatttg ccgagcgcgg cgccgtgccg cgcaatgatg gagcgcatca cctcgacacg
      121 acgggcttct atcacttcga acagctcatc gaacgattcc gaaaaagacg ccacgccctc
      181 ttcggtcaac ttggtggtga ccgccgtcat gtcaacgccg actgccttca gttcgtcgaa
      241 gactcgatgg gcttcctcaa ttccggcatc gagcgtcggc ttcgccgtgc cgtgatcgcg
      301 gaaggcgtcc aaggttgcgg ggggcacggt gttgaccgtg tcggcgccga tcaaggtgtc
      361 caggtacatg gtgtcgggat acgccggatt cttggtgctg gtggacgccc aaagcgggcg
      421 ctgtaccctg gcgccttttt ccttcagctt ggcaaaacgg tcgctgccaa agatgcgctt
      481 gaacgattcg taggctacct tcgcattagc gatcgcagcc ttgcccatga gggcattgat
      541 gcgctgcttt tgctggtcgt cggtggcttt ctcggcgagt tgtccaagct gcttgtccac
      601 catgccgtca atgcggctga cgaagaagct tgccaccgag gctatacggt ccaccggtag
      661 tccggcggcg acgcggcgct cgagtccttt gatgtaggcc tcggcgacct gctcgtacac
      721 atcaaccgcg aagataagcg tgatgtttat gttcaggccg gagaagagca gttcctcgac
      781 ggccggaagt ccggcgggcg tacccggaac cttgatcatt acgttgggcc gattcagatg
      841 ggcgaacagc ttcttggcct cttcgatcgt cgccttggtg tcgttggcaa gcagcggaga
      901 aacttccagg ctgatgaatc cgtcggcgcc gtcattggcg tcgtaaacac cccgcagcac
      961 gtcgctgccg ttgccgatgt cctggaggac gatttcttca tagatttggg agacattgct
     1021 gccgccacgc gccagttttt ccagttgctc ggcatagtcc tggctgccgc tgatggcctt
     1081 ttggaagatg gtagggttcg aggttacccc gcggagaccg tcttcgtcga tgaggcgctt
     1141 gaggcccccg gttgtcaaaa gagaccggct catctgatcg agccaaatac tttgacctaa
     1201 ggcatgaagc tgaagtaagg ggttggtgct cattttatta gccctcgcaa atcttgctct
     1261 tcgagcgcgg taattttcgc cagccgccgg cggtggcgct gttctccgct gaatttcgcc
     1321 cccagaaacg cacgtaccaa gtccaatgcc agctccggtc cgatcacgcg cgagcccagg
     1381 cagagcacat tcatgttgtc atgttcgacg ccctgatgcg ccgaataaca atcgtggcaa
     1441 agtgcggcgc gaatgccggg aagcttgttg gccgccactg acgcgccgac tccactaccg
     1501 cagatgagta ccgcgcgctc agctttgcct tcgagtacgg cgcggcccac ggcctctgcg
     1561 taatcgggat agtcatcggg cttactgggg tcgtgggtgc cgcaatccac cacctcatgc
     1621 cccagccgtt tgagctcggt aatgacggtc tcgttgagtg gaactccggc gtggtcagcg
     1681 cctacggcta tcttcatggt catggtacgc ctgcaagatc aaggtttgat ttttttcatg
     1741 acttcctggg ctttcgccac gacgtcatcc accgtgaatc cgaagtgctt caataggtct
     1801 ttataaggag cagattcccc aaagtccgtc cgagcgacaa catatccgtc atttccaatg
     1861 tattccttcc agccaaaatc ggtgcccgcc tcgaccgcca cccgagctcg cacctcaggt
     1921 ggaagcacct ggcgtttgta ctcgtcggac tgctcttcga acagtcgcca gcagggcatg
     1981 gagaccacac gcgccttcac gccattggca gtgagctttt cataagcgcc gacgcagagc
     2041 tgtacctccg agccggtccc catcaagatg atgtcagggg tgcccccgga atccgccatg
     2101 atgtatgcgc ccttggcaag gccggctgcc gacgcatact tggtgcggtc gaacgtgggg
     2161 actgcctggc gggtaagcac cagcgccacc ggttgcgtct tcaaaggcat gatgtagcgc
     2221 caggtctcgg cgacctcgtt ggcgtccgcc ggccggaaca acagtagccg cggaatggcg
     2281 cgcaaggaca taaggtgttc gatcggctgg tgcgtcggtc catcctcgcc gaggcagaag
     2341 gaatcgtgcg tgtagatcca aatgaccggc tggtccatca tcgccgccag acgaagtacc
     2401 ggccgcatgt aatcggaaaa gacaaagaag gtagctccaa atggacgcag cttggatagc
     2461 gccatgccgt tgaggatgga gcccatggcg tgctcgcgga tgccaaagtg gaagttgcgg
     2521 cccgcatagc tgcccttctc gaagcttgta gtgccgctga gcttggtctt ggtcgaagga
     2581 tccagatcag ccgcacctcc cagcagccac ggcacgttct tcgccactgc gtttagcacc
     2641 ttgccactcg agtcgcgcgt ggccaggcct ttggcatcgg cagggaagga cggaatgtcc
     2701 ttgtcccagc catcgggcaa ttcttgtttc agcatctgct gacaggtctt cgcgagatcg
     2761 gggaattcct ttgcccaggc gttgtagcgg ccatcccagt ctttctcccc ctgggcgccg
     2821 cgctcgacgg ccttgcccat gtaggccttc acctcgtcgg gaaccaggaa ctgcgcatcg
     2881 gggggccagc cgtaaaacat tttggtgagg cgaacctctt cttcgcccag aggctcgccg
     2941 tgagcttcct tggtgtcctg cttatggggg gagccgtagg cgatgtgggt gtcaacgata
     3001 atcagcgacg gacgctccgt ctccttcagt gcggtctgga tggcgcgatc gacctccggg
     3061 agatcgttca cgtcgctcac ctgttgcacg ttccaatgcc agccgaggaa gcgcgtgcct
     3121 acgtcatcgc tgaaggccag cgaggtatta ccctcgatgg tgatgtggtt gttgtcatag
     3181 aaccagatga ggttcgagag tccaagatgc ccggcgactg aggcagcttc gttgctcacg
     3241 ccttccatca aatcgccgtc accgcaaaag gcgtaaacgc ggtagtcgaa gagatcgtgg
     3301 ccgtccttgt tgaattgcga ggccagccag cgctgggcga ttgccatgcc gactgaattg
     3361 ctgacgccct gacccagcgg gcccgttgtg gcttcagctc ccggcaccac tccgtactca
     3421 ggatgtcccg gagtcttgga tccccattgc cggaactgct tgatgtcgtc gagcgaaaga
     3481 tcgtagcccg tcaggtacaa catggagtaa agcagcatgc acgcgtgccc attggacaga
     3541 atgaagcggt cacggctagg ccaatgggga ttgtttggat tgtgacgcag atatcggttc
     3601 cacagcgtaa acgccagtgg cgccaacgcc atcggagttc ccggatgccc gctgttggcc
     3661 ttctgcacgg catccatcga gagcgtacga atggtattga tgcagaggcg gttcagctca
     3721 cactgctcct gcgtttcgtt atcgaaccca tctttggggc ccactgcgat ggag
//
LOCUS       DATMGX010000698         4696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_8161,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000698 DATMGX010000000
VERSION     DATMGX010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8161"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VNR65_11440"
     CDS             <1..235
                     /locus_tag="VNR65_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19328.1"
                     /translation="VQWSCSWSRLSNSPNCAAGGSTFTGLPTGLLNPGQSLIIGQTSY
                     PYTPAIGEFLTGGLTFSSTFYFRPRLVTLVVKQ"
     gene            complement(259..1143)
                     /gene="cpaB"
                     /locus_tag="VNR65_11445"
     CDS             complement(259..1143)
                     /gene="cpaB"
                     /locus_tag="VNR65_11445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HWJ19329.1"
                     /translation="MIFRNALLILGVICIVSGLALGYVWVTSQDAPVEAAPEVRAPEP
                     QPAKIAVLSASHDIASGTLLQQGDMLWKDVAPLEIRPGNLVRGQVTEAEFVGALTKRD
                     FASGEGLISGDLLKHNDRRFLAAALKPGNRAISIPVDAAQSAYGLIEPGNYVDVILTQ
                     SLGDQSSDARRKVVAETVLQNIRVIAVDQTLTQQVKVPAGLQFSSEPRLPKTVTLELT
                     KQQAETVFVALQLGRLQLSVRSTEEVPGGAADHAEQQSPTWASDVSAALKDMPRAPLQ
                     PSTSSLEGSIRRPPGSLQ"
     gene            complement(1140..1679)
                     /locus_tag="VNR65_11450"
     CDS             complement(1140..1679)
                     /locus_tag="VNR65_11450"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HWJ19330.1"
                     /translation="MSFVIIPKILWMISLAALLLSAATDLKERIIPNELVIVIALSGL
                     LLCLTMRPGQICISLMAAALVFLATGVLCRYEALAGGDVKLMAAVTLLVPPGGVGKLM
                     VGIALAGGLLSCVYLGASYMVRNACSASGNRPRDNAVSSVGEWARVESIRIASRKQVP
                     YAVAILGGVIWYVARENLT"
     gene            complement(1770..1961)
                     /locus_tag="VNR65_11455"
     CDS             complement(1770..1961)
                     /locus_tag="VNR65_11455"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HWJ19331.1"
                     /translation="MRRFSDMCRSAYAWFKVESEDGVAAIEYGLLAALIAVVIITAVT
                     LVGTNLTGVFNSIAAKVHS"
     gene            complement(2048..3643)
                     /locus_tag="VNR65_11460"
     CDS             complement(2048..3643)
                     /locus_tag="VNR65_11460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWJ19332.1"
                     /translation="MFVKDGFAQAAKPSRCAGTFGTLRRPVAIMAGLTACTVLVRLPF
                     LSDAGSDECFYLVVARQWLEGLPPYANAFDVKPPLLFALLAGAEALFGPTLFAAKALI
                     MATVSATACALYLFGRRFLGEISGVAAALFYIAASLTLGGTFSPAELIMAPFTAFGML
                     AGFTAIARVRPPLLLVAGAGALLGAGACVKPTVVFEALPLAAFLVFQRPRGDGLKALA
                     SLAAGCLVVPAVFALLFFAEGHFGALFEDVVLSAAGRMGATYVPWGEAVIRFAIELML
                     VLPLVILGASAWVFRGAFRAQRVRPAIGFLAAWTAGALAGVLLGRAMCDFYMLAALPP
                     LCLLSGVFLDHGIARSLAKNTLALARMVAFASVTIFFATVLWCASYNIADSRAADQAA
                     TAMRNAGLRKDDRILVADRDLAVYLASGANPPAPVFHPIQLLCDFAFEKAATAFEDSL
                     KSRPAYIIVADPPYALGCEKPERRALLVLTLANDYRAVGHFGSPRRAKGGMTVYGLKD
                     NEPHPAVASSSDVPQKQLKLYWH"
     gene            complement(3634..>4696)
                     /locus_tag="VNR65_11465"
     CDS             complement(3634..>4696)
                     /locus_tag="VNR65_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19333.1"
                     /translation="LGCLAALTQIRPRLWLLLAAGLCLGAAACVKQTALFEALALAAF
                     LFVGRPLADGLKSVVLLSAGFLVVPLGFAFYFLADGHFAAFFADAVIAAVGRVSVFYL
                     GWNETLTGLLLGLMLMLPVIILAAAAWVFPGGSKSSALPFVGAWTAGALSGVLATRAA
                     LVIYFLPLLPPLCLMAGAFVQHWGTRIPGRKRLVMAAAITAVAAYSAFFTAPLFFAGR
                     DNLASAHEAAAAMRGAGLKSDDRILVADRDLIVYLASGANPPKVVFHPLHLLCGFPLP
                     DNANALADSLKSRPAFIVEANPLYARACEIPERRLLLDGALSRDYCQLGQFRNTLTGA
                     GSGSLVVFGLKERAGARCL"
BASE COUNT         1116 a         1448 c         1361 g          771 t
ORIGIN      
        1 cgtccagtgg agctgctctt ggagccggct gtcgaactct ccaaattgcg ctgctggcgg
       61 aagcaccttt acggggcttc ccaccgggct tctcaaccca ggccaaagcc tgatcatcgg
      121 ccagacatcg tacccctata cgccggcaat cggagaattc ctgacgggcg gcctcacctt
      181 ttcgtctacg ttctatttca ggccgcgcct cgttaccttg gtagtgaagc agtagtcaat
      241 agtcgaccgc atgtgacatt attgtaggga gccaggcggc cgtcgaatgg aaccctcgag
      301 tgagcttgtc gagggctgaa ggggcgcacg aggcatatcc ttcaaggctg cggagacgtc
      361 gctcgcccat gtcggggact gctgctccgc atggtccgct gccccccctg gaacttcctc
      421 cgtggaacgg acggaaagct gaagccgtcc aagctgcaat gcgacaaaga cagtctccgc
      481 ctgctgtttc gtaagctcaa gtgttaccgt tttgggaagc ctgggctccg atgagaactg
      541 aagacccgcg ggtaccttca cctgctgggt gagtgtctgg tcgaccgcga tgacccgaat
      601 gttctgcagg acggtttcgg caacaacctt ccgcctggca tcgctcgact gatcgcccag
      661 gctttgcgtt aatatcacat cgacgtagtt gcctggctcg atcagcccgt acgcgctttg
      721 agccgcatcg acgggaatcg atatagcgcg gttcccaggt ttcagggccg ccgccaggaa
      781 ccgcctgtca ttatgcttca agagatcgcc cgagatcagc ccctcgccgc tggcgaaatc
      841 gcgcttcgta agagcaccaa cgaactcggc ctctgtaacc tggccacgaa cgaggttgcc
      901 tggacgaatc tccaaaggcg caacgtcctt ccaaagcatg tctccctgct gaagcaaagt
      961 gccagaggcg atatcgtggg aggcgctcag tacggctatc ttggcgggct gcggttccgg
     1021 cgcgcgcact tccggcgcgg cttcaacagg agcatcctgg ctggtcaccc agacataacc
     1081 gagcgccaat ccgctcacga tacagatgac gcccagaata agcagggcat tgcggaatat
     1141 catgtcaggt tctctctagc gacataccag atcacaccgc caagaatagc gacggcatag
     1201 ggcacctgct tgcgcgaggc gatccgaatg ctctcaaccc tcgcccattc gccaacactg
     1261 ctcacggcgt tgtccctggg tcgatttccc gaagcgctgc aagcgtttcg gaccatgtaa
     1321 ctcgcgccga ggtacacgca gctcaaaagc cccccggcca gagcgatccc gaccataagc
     1381 tttccaacgc ccccaggcgg cacaagaagc gtcacagcgg ccatcagctt cacatctccg
     1441 ccggcgagtg cctcgtagcg gcagagaacg cccgtggcca aaaagacgag cgcggccgcc
     1501 atcagactga tgcagatttg accgggccgc attgtgaggc atagcaagag ccccgatagg
     1561 gcgatcacaa tcaccagttc gttcggtatg atgcgttcct tcagatcagt tgccgcgctc
     1621 aaaagaagag cagccagact gatcatccag aggatcttcg gtatgatgac aaaactcatg
     1681 tcgtcctcga cgcgatcaaa ggagcgccgc ggagagggct catgcttctc cgggcgcgat
     1741 cgcggtcagc ctacccagac aacaagattc tagctgtgaa ccttggctgc gatggagttg
     1801 aagacgccag taaggttcgt tcccaccagc gtcacggcag tgatgatcac cacggcgatc
     1861 aaagcggcga gcaagccata ttcgatcgcg gcaacaccat cctcggattc gaccttgaac
     1921 catgcataag cggacctgca catatcagaa aaacggcgca tacaactctc cctgctacta
     1981 gttgtggaac cttactggta gcacggataa attgctgttt tgccaagccg acgctggtcc
     2041 tttgccatta atgccagtat agttttaact gcttttgagg tacatcggac gacgacgcaa
     2101 ccgcaggatg aggctcattg tccttcagtc cgtaaaccgt catgccaccc ttggctcggc
     2161 ggggagaccc gaaatgccca acagcgcggt aatcattcgc aagagttaag acgagtaacg
     2221 cacgccgctc cggcttctcg caaccgagcg cgtagggggg atcggccacg ataatgtaag
     2281 ccggacggct tttgagggaa tcctcaaatg cggtcgccgc cttttcgaac gcgaaatcgc
     2341 aaaggagttg aatcgggtga aagacaggag ccggtggatt tgccccggac gcgagataaa
     2401 cggccaaatc gcggtcggct acgaggatac gatcatcctt gcgaagaccg gcgtttcgca
     2461 ttgccgtagc ggcctgatct gcagctctgc tatcggcaat gttgtaagac gcgcaccata
     2521 gcactgtcgc aaagaaaatc gtgacgctgg caaatgccac catccgcgcc agcgcgagcg
     2581 tgttcttcgc cagagagcgc gcgatcccat ggtccaggaa cacgcccgac aaaaggcata
     2641 agggaggaag cgccgccagc atgtagaagt cgcacatcgc tctccccagg agcacgcctg
     2701 cgagcgcgcc cgcagtccag gccgcgagga agccaatggc gggacgaact cgctgcgcgc
     2761 gaaatgcccc gcggaaaacc catgccgacg cccccaggat cacgagtggc agcacgagca
     2821 taagctcgat cgcaaaccgt atgaccgcct cgccccaagg cacgtaagtc gcacccattc
     2881 gcccggcagc cgaaagcacg acatcttcga acagcgcgcc gaaatgtccc tcggcaaaga
     2941 aaagaagggc aaataccgcc gggacaacga gacacccggc agccaatgaa gccagcgcct
     3001 ttagaccatc gcctcgtggg cgctgaaaaa ccaggaatgc cgccaacggc aaggcttcga
     3061 agacgactgt cggctttaca caggcccctg cgccgaggag cgcccccgcc cctgctacta
     3121 gcaacaaggg aggacgcacc cgcgctatcg cggtaaagcc cgcaagcatg ccgaaagccg
     3181 tgaacggagc catgatgagt tcggctgggg agaaggtgcc gcccagcgtc aagctagccg
     3241 cgatatagaa aagcgccgcc gcgacacccg aaatctcacc gagaaagcgc ctcccaaaaa
     3301 gatacagagc gcaagctgtc gccgacacgg tggccatgat gagggccttg gccgcgaaca
     3361 acgtcggtcc gaaaagggct tccgccccgg ccagaagagc aaacaaaagg ggcggcttca
     3421 catcgaaggc gttggcgtaa ggtggcagcc cctcaagcca ttggcgcgcg accacgagat
     3481 agaagcactc gtccgagccc gcgtcagaga gaaaaggaag ccggaccagc accgtgcaag
     3541 ccgttagccc cgccatgatc gcaactggcc ggcgcaaggt tccaaatgta cccgcgcacc
     3601 gggacggctt ggcagcctga gcaaagccgt ctttcacaaa catcgtgcgc cagcccgctc
     3661 cttgaggccg aagacaacca atgagcctga ccctgctcct gtcaaagtat ttctgaactg
     3721 cccgagttga cagtaatcgc gactgagcgc gccatcgagg agcaggcgcc gttcggggat
     3781 ttcgcaggct cgagcgtaga gggggtttgc ctccacaatg aacgctgggc ggctcttgag
     3841 cgaatccgcc agagcgttcg cgttatcagg caagggaaag ccgcacagaa ggtggagggg
     3901 atggaaaacg actttcggcg gatttgctcc cgacgccagg taaacgatca gatcgcggtc
     3961 tgcgaccagg atgcgatcgt cgcttttaag gcccgcgcca cgcattgccg ccgccgcctc
     4021 atgggctgac gcaaggttat cgcgccctgc gaaaaacaag ggagccgtaa agaaagcgga
     4081 ataggccgcc acggcggtaa tcgcagccgc catgacgagg cgtttgcgtc ctggaatgcg
     4141 agtcccccaa tgctggacaa acgcgcctgc catgaggcac agcggcggaa ggagcggcag
     4201 aaagtaaatg acaagcgctg ctctcgtcgc cagcacgccg ctaagcgcac ccgccgtcca
     4261 ggccccgacg aatgggaggg cggaactctt cgatccgccc ggaaaaaccc atgctgctgc
     4321 agccaggatg atgacgggca gcatgagcat cagaccaagc aagagcccgg taagggtctc
     4381 gttccatccg aggtaaaaca cgctcacgcg tcccacagct gcgatcacgg catctgcaaa
     4441 aaacgccgca aaatggccgt cggcaagaaa gtagaaggca aatccaagag gcacgacgag
     4501 gaagccggcg gatagcagaa ctaccgactt caggccatcg gccagcgggc gcccaacaaa
     4561 gaggaaggca gccagagcca acgcctcgaa aagggctgtc tgcttcacgc aggccgcagc
     4621 gcccaggcaa agccctgcag cgagtaagag ccatagacgc ggccgtattt gcgtaagggc
     4681 agccaggcag ccgagc
//
LOCUS       DATMGX010000699         3061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_229061, whole genome shotgun sequence.
ACCESSION   DATMGX010000699 DATMGX010000000
VERSION     DATMGX010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229061"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VNR65_11470"
     CDS             <1..1352
                     /locus_tag="VNR65_11470"
                     /inference="COORDINATES: protein motif:HMM:NF041166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment cargo
                     protein cysteine desulfurase"
                     /protein_id="HWJ19334.1"
                     /translation="PMNVPPSAGGFSSSAPGSHFYFLDESRISSARTSPSASQAGYDV
                     HAVRRDFPILDQRINGHPLIWLDNAATTQKPQAVIDRLVHFYSFENSNVHRGAHELAA
                     RSTDAYEDAREKVRGFLGASSSDEIVFVRGATEAINLVAQSWGRQNIGKDDEIVITWL
                     EHHANIVPWQQLCTETGAVLKVVPVDDHGQVRLDAYEKLLGPRTKLVSFTEVSNALGT
                     VTPAKQMVEMAHRYGARVLVDGAQAVSHMPVNVQELGADWYAFSGHKVFAPTGIGVLY
                     GRKDLLDSMPPWQGGGNMIRDVTFERTEYHDAPGRFEAGTGNIADAVGLGAAVDYLGK
                     IGMEKIAAHEQGLIAYATKLLADVPGLRIIGTAAEKAGVVSFVLNGQQSEGVGAALNQ
                     EGIAVRSGHHCAQPILRRFGLESTVRPSFALYNTYADIDALAAALRKIQSGHNPYMG"
     gene            1548..1727
                     /locus_tag="VNR65_11475"
                     /pseudo
     CDS             1548..1727
                     /locus_tag="VNR65_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028169.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(1847..2299)
                     /locus_tag="VNR65_11480"
     CDS             complement(1847..2299)
                     /locus_tag="VNR65_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19335.1"
                     /translation="MAGNSSKFKVVAFEDGTHAVVRTDPSNPCFMEVVAAFYDASRAR
                     DYADVENHQASEQQRSIAPSTSPKKEGVAASEGAPEVSQRQRAVLEAFRRKMNGSNLV
                     EVKAAVLAEAAQIPLGSLHSVLQSLEKKQLIKTARGGSARAPAVYQVL"
     gene            complement(2616..2870)
                     /locus_tag="VNR65_11485"
     CDS             complement(2616..2870)
                     /locus_tag="VNR65_11485"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWJ19336.1"
                     /translation="MDPIAIIVILVIGAVAGWLAGVIYTGAGFGLVGNIIVGILGALV
                     AGALLPGFLHFSGIVGQIISAAIGAIILLALVNFVSRRAA"
BASE COUNT          633 a          907 c          902 g          619 t
ORIGIN      
        1 ctccaatgaa tgttcccccg tcggctgggg gcttcagttc cagtgcgccg ggctcccact
       61 tctacttcct cgacgaaagc cgcatatcct cggccaggac aagcccatca gcctcgcaag
      121 ccggctacga cgtccatgcc gtgcgccgcg actttcccat cctcgaccag cggatcaacg
      181 gccatccgct gatctggctc gacaacgccg ccaccacgca aaagccgcaa gcggtcatcg
      241 accggctcgt gcatttctac agcttcgaga actcgaacgt gcatcgcggc gcgcatgagc
      301 ttgccgcgcg ctccaccgac gcttacgagg acgcccgcga aaaggtccgc ggttttcttg
      361 gcgcgtcgtc ttcggacgag atcgtgtttg tccgcggcgc aaccgaggcc atcaacctcg
      421 tcgcgcaaag ctggggccgc cagaacatcg gcaaggacga cgagatcgtc atcacctggc
      481 tcgaacatca cgccaacatc gtgccctggc agcagctgtg cacggagacg ggggcggtgc
      541 ttaaagtggt gcccgttgac gaccatggcc aggttcggct cgatgcctat gagaagctgc
      601 tggggccgcg taccaagctc gtctccttca cggaggtttc gaacgcgctc ggcaccgtca
      661 cgcccgcaaa gcagatggtg gagatggcgc accgctatgg cgcgcgcgtt cttgtcgatg
      721 gcgcgcaggc cgtctcgcac atgcccgtca atgtgcagga gctcggcgcc gactggtacg
      781 cgttttcggg ccacaaggtg ttcgcaccga ctggcatcgg cgtgctttac ggcaggaagg
      841 acctgctcga ctcgatgccg ccatggcagg gcggcggcaa catgatccgg gacgtgacct
      901 tcgaacgcac cgaatatcac gatgcgccgg ggcgtttcga agccgggacg ggcaacatcg
      961 cggacgcagt gggcctcggt gctgccgtcg actatctcgg caagatcggg atggaaaaga
     1021 ttgccgccca cgagcagggg ctcatcgcct atgcgacgaa gctcttagcc gatgtgcccg
     1081 gcctgcgcat catcggcacg gccgccgaga aggccggcgt cgtctccttc gtgctgaacg
     1141 gccagcagag cgaaggggtc ggcgcagcgc tcaatcagga aggcatcgcg gtgcgttcgg
     1201 gccatcactg cgcgcaaccg atcctccgcc ggttcgggtt ggaaagcacg gtgcgcccgt
     1261 cgtttgcgct ctacaatacc tacgccgaca tcgatgcgct ggcggcggct ttgcgtaaga
     1321 tccagagcgg gcataatccc tatatgggct gagacatgga caagcctggt gcttattgcg
     1381 ccgggcttgc tatcagcctg tccctgcgtg gcaacggctc tcggagcgac gccccttgac
     1441 cgctgacgcg atcgatgcgc cgccgaagac cccgacaccg tcgccgccaa ctcgctccag
     1501 caatcgcatt cggtgcgcga agcggcgcgc cagattgggt cgccaagaac acctctttcc
     1561 gctggcgcca ccgctttcta cgcctggaca cagacacgct taaacagaag cttaccggca
     1621 tcgtcgaggt tgacgagagt tttattctgg aatgccgcaa aggcgagcgc gggctgccaa
     1681 gagaggcgcg caaacgcggc ggcaaggcta aaaagcctgg gctttcaatg gcgtagccag
     1741 caaatacctg ccgaattatc tcgcttggcg aaggcggttg gagaccggaa aaattccctt
     1801 cgctgcaata ccttgctgaa tacaacgttt aactacgaca gagccatcac aaaacctggt
     1861 agaccgcagg cgctcgtgcg gagcccccac gggcagtttt gatgagctgt ttcttttcta
     1921 gcgattgcag aaccgaatgt agagagccga gaggtatttg tgccgcctcg gccaagacgg
     1981 cggcctttac ctcaaccaag ttgcttccgt tcattttcct gcgaaacgct tctagcactg
     2041 cgcgttggcg ctggctaacc tccggcgcgc cttcacttgc tgccactcct tctttttttg
     2101 gagaggtgga aggcgcgata cttctctgct gctcggacgc ttgatggttc tctacgtctg
     2161 cgtagtctcg ggcgcgtgag gcgtcgtaga aagcagcgac cacttccata aagcaagggt
     2221 tgctcggatc cgttcgcact acggcgtgag tgccgtcctc aaatgcgacg actttgaact
     2281 tcgaactatt gcctgccatg atcttatcct tttctgacgg cgagcgtggc gctccctttc
     2341 cctgttgtcg tttgtatcgt tcgagatgtt agcggggcaa tatacgatta atgaatgata
     2401 acttttgcaa aactaatatc ggtggactgg tcgcggggac atagttcaag cggcggagca
     2461 cggaagggta atatagcggg ggaggatggg ggaggggtgc aacgtgcgcg ttcggactgg
     2521 agcaagttgc attggagagc ggaagaaatg taagggcaag tatggcgctt gccctttttc
     2581 gacggtaatt ttctgcggca gccgcagcta acgcttcaag ccgctctgcg agaaacgaag
     2641 tttacgagtg ccagcagaat gatagcgcca atcgcggcgc tgatgatctg gcctacgatg
     2701 cccgaaaagt gcaagaagcc cggcaacaaa gcgcccgcga cgagagcgcc gagaatgccc
     2761 actatgatgt tgccgacaag cccgaatccc gcgccggtat agattacccc ggcaagccat
     2821 cctgcaaccg cgccgatgac taagatgaca ataattgcaa taggatccat aggaactctc
     2881 ccttgaagca gttcgcgttc tgaaactctg ggtgttgaag atagcgcatc aacaacgctt
     2941 tactttctcg cgcttgagca agctctccta tgacgaaagt caataccaac tatctttttg
     3001 gatggaacaa gctacgcttt cgttcgtttg gcgatgttgt ttttcgcaac tcaagcggaa
     3061 a
//
LOCUS       DATMGX010000700         2701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_171487, whole genome shotgun sequence.
ACCESSION   DATMGX010000700 DATMGX010000000
VERSION     DATMGX010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171487"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            680..865
                     /locus_tag="VNR65_11490"
     CDS             680..865
                     /locus_tag="VNR65_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HWJ19337.1"
                     /translation="MSYDQFPDRLSRPMSRGQAATLRTLSVEAYQPKLFEKNLTVEEA
                     DRRIEALRQEIALANSF"
     gene            1045..1239
                     /locus_tag="VNR65_11495"
     CDS             1045..1239
                     /locus_tag="VNR65_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19338.1"
                     /translation="MDTNFLKAHAERCRSLAENADEFTKRRLLDLAATYDKRVWASGP
                     SAATRILNAPLNLVETPKQI"
     gene            complement(1441..1704)
                     /locus_tag="VNR65_11500"
     CDS             complement(1441..1704)
                     /locus_tag="VNR65_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19339.1"
                     /translation="MLDHDHALCVPITPVSIMVSFTTHFDTHTAAVTISIAMHLAAVT
                     ITVVAITTDADAKLFGTRDGRSSDRNSREGGNNKSKFFHLVSF"
     gene            2030..2275
                     /locus_tag="VNR65_11505"
     CDS             2030..2275
                     /locus_tag="VNR65_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19340.1"
                     /translation="MSHRSAIVSLHVEARILLTTVAGRSSMDRMAEGIEHMVIETLLF
                     IAFIVIVGAAIVKAYEWHHDVLYGPYIKTGRLKQKPR"
BASE COUNT          637 a          748 c          734 g          582 t
ORIGIN      
        1 cggatatttc atccgctgct cgcggacaca cttgtcccgt tcggcacata ggttgtcgac
       61 ggtggcctgc atgcgtgcca acagagcttc cgcaggcaac gtgctgatcc cggcgcggct
      121 atgctcaagg ttcattgcga agtccaattt tcggtactgg ccgaaatagg ccacccgtga
      181 agcaaaacaa accacgcgac gaaaaacccc gccggaggca gccagcgggg tttcttctta
      241 tttgtggtca ggtgcgccgc tgagaactgg cgcaagggta tactagccaa aagtattaaa
      301 ttacagtccc ggggaacggc agtcgaagtg gcccgttatc gcgtgtcgcg cccccccaaa
      361 catctcgaca tcccagaacg gccccagctg ctcccccgca ctggggccgt tgctttgggg
      421 accactcggg aaagcgaaac ggcccactcg tatgggctag ttgaccgggc acctcaagga
      481 accgatctcc gtatttatac ggagaacgtt taggggtgga ttaactactc ctgctagggt
      541 gcatcattca aattaggcca gtgttgattt caacttacgc cgaggccgga aacaggccag
      601 acccaatttt tcttttagaa caaaaagaga acatgtttgc aagttacaaa ttaggataga
      661 tgctgaaatt ggagccgcca tgtcttacga ccaattcccc gatcgcctct cccgcccgat
      721 gagccggggc caagctgcta cattgcgcac gctctcggtc gaggcgtacc agccaaaact
      781 gttcgagaaa aatttgaccg tagaggaagc cgaccgacgc atcgaagcgc tacgccaaga
      841 gattgcgctg gcaaattcat tttaataccg gcaacgctgg gcgtttcgca atgagcgttc
      901 acctgaaacc atgctttgtt ctcgtggaac gaacaggggt caggatttct cattcatcca
      961 cagcttaaca aagtctctcc gaagcccatg gattgaccgg gtcggctgtg caaaattgaa
     1021 tgcgttgttg agtgggggac tgccatggac accaattttt tgaaggctca tgccgaacgc
     1081 tgtcgcagcc tcgcggagaa cgcagacgag tttactaagc gacgattgct agacctcgcg
     1141 gcaacctacg ataagcgggt ttgggcttca ggaccctcgg cagcaacacg catcctcaac
     1201 gcaccgctca atctcgtcga gacgccaaag caaatctgag ctttgctgct gaggttgatt
     1261 tctccctgaa ggccgcgcgg aatgcgcaca ttccggtttg acttgaagct tgcgcctcgt
     1321 tgaggcggcc ccggctttgc cggcataacg taattactga acgaccattc ataaaaaacc
     1381 acgtatccgc gacggaacgt tccggttctc cggcaatttc ctttgtgtcg aacatccagt
     1441 tcagaaggaa acaagatgaa aaaacttact tttgttattg ccgccctcgc gactattgcg
     1501 gtcgctgctc ctaccatcgc gagtgccgaa gagtttggcg tccgcatcgg tggtgatcgc
     1561 gaccacggtg atcgtgaccg cggcgagatg catcgcgatg gagatcgtaa ccgcggcggt
     1621 atgcgtgtcg aaatgcgtgg tgaacgacac catgatggag actggcgtaa tcggcacgca
     1681 gagagcgtgg tcgtggtcaa gcatcgtcgc catcatcacg aagactgaaa atgcggaaga
     1741 tggcccctct caggggccat tttttgaccg cgatcctttt tcagagtggc tgaacgcatg
     1801 ccctaccttg aaggccgctt tctagtagca aggtgacaag ttaaaacgct cgaacgccat
     1861 gtgctcgctg agaactaacc ttgagcgccg cgccgacctc caagataact tttgtggcaa
     1921 ggacgggcga ccggcgtcga gccgcaaaca tctgtccgtt ccttctcgct gaacggaacc
     1981 aaattcaagc ctctcgatca gttccagccc gtgagcaggc gctgcaaaaa tgtcgcatcg
     2041 ctcggcaata gtctcgcttc acgtcgaagc ccggatttta ttgaccactg tggccggtcg
     2101 atcctctatg gatcgtatgg cggaaggtat tgaacacatg gtcatcgaaa cgcttctgtt
     2161 tatcgctttc atcgtcatcg tcggggccgc catcgtgaaa gcctacgagt ggcaccatga
     2221 cgttctgtac ggcccctaca taaagacggg tcgattgaaa cagaagccac gatgatagcc
     2281 atcagcttca cccttggatt gcgccaactc cccgcaacga caatggtctg gcatttatgc
     2341 acggtcatca tcccgagacg cacaatcacc ggccggcttg tgcgcggaca agtatggcgg
     2401 cgccacgacg gacgtcactg gttatataaa aaattcatcg cttagggatt ggcgttgcaa
     2461 actattggcg ggggcgtgcg atgatgaaat agccctccca cgagcaccaa gacggtggcg
     2521 gcgggggagg gaaagccgcg agcgcgtcca acccgctaat gaacggcgcg tctgccgaag
     2581 gtgtagtgat aactcgtcaa tcatattgac tgttgagcgg ttgctaacca ccgctgttaa
     2641 gcgtttcgac ggtcccatct gcgatgttgc tgaaacacgg gcaccaagac ccgtcaggga
     2701 a
//
LOCUS       DATMGX010000701         3665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_263461, whole genome shotgun sequence.
ACCESSION   DATMGX010000701 DATMGX010000000
VERSION     DATMGX010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263461"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VNR65_11510"
     CDS             complement(<1..456)
                     /locus_tag="VNR65_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19341.1"
                     /translation="MKRKAKKSTRQKGKFVTVNDSMQKGYCYALSTRVGRNFDPEFKP
                     ELTPQQMLTLGVFCGKYMTDTRKEFPKSWFKRAKLSPSGRDCSLNYFGVDASRPLSEW
                     RNKGWINPDDPRGWFQWYCRYYMGRRVPDEDRRQIKRWKAFRRHIAQVRM"
     gene            complement(509..1012)
                     /locus_tag="VNR65_11515"
     CDS             complement(509..1012)
                     /locus_tag="VNR65_11515"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HWJ19342.1"
                     /translation="MTQSYERSTFKCDVAFAVFAVGAVAATSILGQIATYPNLSPWYA
                     GLAKPSFNPPNWVFAPVWTILYALMAFALWRILRMYGPSGIRRSAIALFLILLAFNAA
                     WSWMFFWAHSPLLGLVNIVPQSAVIVATIVVFAQLDGLAAICLVPLAAWVGFASVLNI
                     AIWRLNG"
     gene            complement(1317..1922)
                     /locus_tag="VNR65_11520"
     CDS             complement(1317..1922)
                     /locus_tag="VNR65_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19343.1"
                     /translation="MSQESSMHVYLNWTKERIDEMDATLASLEAKATQVKADSKVKAD
                     QLIADLKKRRDEFRANAKAQAQAGEATLHAAKAQLESQWKGFETQVKAYFDTVGKQIE
                     QQQATFRDVAAAQAKAWREAADKLQAEAVKVTAAKRADIDAAVKQIKADATEAEARLQ
                     KLKQAGGESWAVLSGALAESRKTFDQASQKAWETLKRASAA"
     gene            complement(1969..2547)
                     /locus_tag="VNR65_11525"
     CDS             complement(1969..2547)
                     /locus_tag="VNR65_11525"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HWJ19344.1"
                     /translation="MTSQASSSVTVIAFPQLVARNWWLFLLRGFAAITFGVLSLIWPG
                     ISLVTLILLFGAYALVDGVFALAAAIVGRGNAELRWWLVLVGLLGVGIGITIFLWPGL
                     TALTLLYFIAGWIVATGLLQVIGAIELRKAIENEWWLILDGILSVLFGVLLFIMPGAG
                     AVALIWLVAVFAIAFGILMVGFAFKVKNFEKA"
     gene            2719..3426
                     /locus_tag="VNR65_11530"
     CDS             2719..3426
                     /locus_tag="VNR65_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ19345.1"
                     /translation="MAKTQSHERPYHHGDLRRAIVKAALEILHETQSLEFSLRDLARR
                     AGVSHNAPYKHFADKRELLTAVSAAGFEMLTKRMAHKTAGLSNAREQLFAMLRAYIDY
                     GVESPALYNLMFGGYLGGPDRSRPAIELAEAEKTKALLKGVIVAGGLGRAIPDTSRNE
                     RKIAGAILACWSLVHGLTLLLADGLVGPRKKSGALSDSLVQGMLDGLAVELPVLPPGT
                     WVGPQVPESPRPATSNA"
BASE COUNT          791 a         1085 c         1022 g          767 t
ORIGIN      
        1 cattcgcact tgagcaatgt ggcgccggaa ggccttccag cgcttgatct ggcgcctgtc
       61 ttcatccggc acccggcgac ccatgtagta acggcaatac cattggaacc agccgcgcgg
      121 atcgtccggg ttaatccatc ccttgtttcg ccactctgag agcggtcggc tggcatcaac
      181 accgaaatag ttgagcgaac aatcgcgacc ggatggcgaa agcttcgcgc gcttgaacca
      241 gcttttcgga aactctttcc gcgtgtccgt catgtactta ccgcagaaca cgccgagggt
      301 gagcatttgt tgcggtgtca actccggttt gaactctgga tcaaagttgc ggcccacgcg
      361 cgtcgagagt gcatagcagt accccttttg catgctgtca ttcactgtga cgaacttgcc
      421 tttctgcctt gttgatttct tggcttttct tttcatcgaa caattctgaa agcaggacgt
      481 ttcttcctcg atatggccac gtagcgcttc agccgttcaa ccgccagatc gcgatgttta
      541 gaacactcgc aaagccaacc caggccgcga gcggcacgag acaaatcgct gctaggccat
      601 caagctgcgc aaagacaacg atggttgcca caatcacagc cgattgtgga acgatgttta
      661 ccaggcccag tagtggacta tgcgcccaga agaacatcca cgaccacgcg gcgttgaatg
      721 cgagcaaaat gagaaacaga gcaatcgctg agcgacgtat gccggaaggc ccgtacatac
      781 gcaaaatgcg ccatagcgcg aaggccatca gagcatagag tatggtccag accggggcga
      841 atacccagtt cggcggattg aaagagggct tggcgaggcc agcataccac ggcgaaagat
      901 ttggataggt cgcgatctga ccgaggatcg aggtcgcggc aacggcacct acggcgaaca
      961 cggcgaatgc gacgtcgcac ttaaatgtcg atcgctcgta tgactgggtc atgcgaatgt
     1021 caccttatcc tgagccactc tagctctcca tcgaaaacgg ccataggccg agacagtctg
     1081 aaaattgaag tccgtcattg gagcccgcga ccaatcgaag tgtcgcttta gggtcattag
     1141 cggacacggc gagaggtcag cgctatgtct gctttacccc cgaaagtgga catttgcgga
     1201 cgcggttgga atgtccgcta agtgccaaat ccggacatca caggcttgat tgaaatgaaa
     1261 gaggccgcca gctgaggcgc cctctggaat tgatctagcg cagctcagtt ttgcgttcag
     1321 gctgccgaag cgcgcttgag agtttcccaa gccttctggc tcgcctggtc aaaggtcttg
     1381 cgcgattccg ccaaggcccc gctcagcacc gcccaggact caccgcccgc ctgcttcagt
     1441 ttctgaagcc gtgcttcggc ttccgttgcg tccgccttaa tttgcttgac ggcggcatcg
     1501 atgtcggccc gcttcgcggc cgtgaccttg actgcctcgg cctgaagctt atcggccgcc
     1561 tcgcgccaag ccttcgcttg agcggcggca acatcacgga atgtggcctg ttgctgctcg
     1621 atctgcttgc cgactgtgtc gaaataggct tttacctgcg tttcgaatcc cttccactgc
     1681 gattccaatt gcgctttggc cgcgtgcaaa gtggcttcgc ctgcttgcgc ctgcgccttg
     1741 gcgtttgctc tgaactcgtc acgccgtttc ttcagatcgg cgatcagctg gtcggctttc
     1801 actttcgagt ccgctttcac ctgtgtcgcc ttggcttcga gggatgccaa tgtggcatcc
     1861 atctcgtcaa tgcgctcctt cgtccaattc aggtaaacgt gcatgctgct ttcctgtgac
     1921 atcgctatct ccatttgttc aaacatattc cctgatacct tgattggttc aggccttttc
     1981 gaagtttttg accttgaagg cgaatccgac catcaagatt ccgaaggcga tcgcaaagac
     2041 tgcgaccagc catatcaaag ccaccgcgcc ggcccccggc atgatgaaaa gaagaacacc
     2101 gaacagcacc gacaatatgc cgtcgaggat cagccaccat tcgttttcga tggccttgcg
     2161 aagctcaatg gcgccgatga cctgcaacag gcctgtcgcg acgatccagc cagcgatgaa
     2221 ataaagtagc gtcagcgcgg taaggccggg ccacaagaat atcgttatgc cgatgccgac
     2281 gccaagcagg cccacaagca cgagccacca tctgagctcg gcgttgcccc tgccgacgat
     2341 ggccgccgcc agcgcgaaga cgccgtcgac tagagcgtag gccccgaaaa gcaaaataag
     2401 cgtcacaagg ctgatgccgg gccatatcag tgacagcact ccgaaggtga tggccgcgaa
     2461 gccgcgcaga agaaaaagcc accagttgcg tgcgaccaat tgcggaaacg cgataacggt
     2521 tactgaggac gatgcctggc tagtcatgac taatactccc acccggtcgc ttcatatgtt
     2581 gacagtgaca acttctaggc ctaaaagttg gcgccgtcaa cattaggcca gcctacaaag
     2641 tggaatggct gccatgcgtt atgttgacac cgacaacttc gtggcgtaac cgcgtggcaa
     2701 caaaggtgat tttcatccat ggccaaaacc cagtcccacg agcgtcccta ccaccatggc
     2761 gatctgcgcc gtgcaatcgt caaggccgcg ctggaaatcc tgcatgaaac ccagagcctt
     2821 gagttttccc tgcgtgacct cgcccggcgc gccggcgtca gccacaacgc cccgtacaag
     2881 cactttgccg acaagcgtga gctattgacg gccgtctcgg cagcggggtt tgagatgctg
     2941 acgaaacgca tggcccataa aacggcaggg ctcagcaatg cccgcgagca gttgttcgca
     3001 atgttgcgtg cttacattga ctatggcgtt gaaagtccgg cgctctacaa tttgatgttc
     3061 ggaggctatc tgggcggtcc ggatcgtagc cgccccgcta tcgaactggc cgaagccgag
     3121 aagaccaagg cgctgcttaa gggcgtgatc gtcgccggcg gtctaggacg cgccattccg
     3181 gatacctccc gcaacgaacg gaagatcgcc ggcgcgatcc tggcgtgctg gtccctcgtg
     3241 catggactca ctctgctgct ggcggatggg ctcgtcggac cgagaaaaaa gtctggcgcg
     3301 ctgagcgaca gcctcgtgca gggcatgctc gacggattgg cggtcgaatt gccggtgttg
     3361 ccgcccggca cctgggtcgg cccgcaggtg cccgaatcac cgagaccggc aacctcgaac
     3421 gcatgaccga ctacagcgcg atgttcgatt ccggtaatcc ggcggacctt tgaagttgtt
     3481 gaggttcgag caagcttgca atgcgctgag aactttaagg cctcgaggga gggatattcc
     3541 gccgattacc cacttgttcc caatttaccg gcactttcgc taacgcatgc gacgccgttg
     3601 gtacttacgc tggtacaatg gcgccgcggt ctgcaaggcg caagccccgc ggtgcgcggt
     3661 gcgcg
//
LOCUS       DATMGX010000702         4716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_457316, whole genome shotgun sequence.
ACCESSION   DATMGX010000702 DATMGX010000000
VERSION     DATMGX010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457316"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /locus_tag="VNR65_11535"
     CDS             complement(<1..1558)
                     /locus_tag="VNR65_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19346.1"
                     /translation="MHQIARRIRYVLRWAGILAGSLIIAAGALAVILPVVVDAEAIRG
                     TLSQSLSAWSGGPVSITGPIRIGSFANLSVEATGIALKSMPEQGPIRIARAKSVTAVV
                     RLSSLLRGKLEFKKFVVASPHVVFRRGFPEPAQTAYGLGAARLAMALSAGSPFADVEL
                     RDPVLFFTASANGAYERAELSRVRLSKDAVSSISTGVFVKNAPGSESALYALDVTSRG
                     FEVSFRGQCAGACQTAFGSLRLRAALGVGGGARILAAVAPWERPETIALSGELTLSRR
                     RAALDNAAISFGDHAAKGSLALDMTGVRPRLEGTIAYDTLDVTPAWISGSGSFAENPP
                     LAALPLARRETDRPLDFDLRVSADRFRAASFETGPLALALTHIQDRLSVDIASVAVFG
                     GHATGRLDINPGQPASLFLRTSGSQLDAKALASAFQLPFGFSGPVMLQAALTMPVTMR
                     EPAEDVSAAAGTFSIRFPAGGSLEGDVAQTLGTALLEQDLGWGLRGSSFPFTVASIDG
                     IVKPGSLDLKV"
     gene            1977..2708
                     /locus_tag="VNR65_11540"
     CDS             1977..2708
                     /locus_tag="VNR65_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HWJ19347.1"
                     /translation="MKKVFPDAHAAVAGLIKDGMTIMSGGFGLCGIPENLIEAVRDSG
                     AKNLTIISNNCGVDGIGLGVLLETRQIKKMISSYVGENKLFAQQFLSGELELEFTPQG
                     TLAERIRAGGAGIPAFFTKTGYGTIIAEGKETREFDGEHYVMERGLTADIALVHAFKG
                     DNEGNLTYRKTARNFNPMMATAAKITIAQVEHLFHAGEISPDEVHTPGIFVQRLIRVS
                     DAKKHIEQRTTRKHTSPAAATGGDL"
     gene            2708..3346
                     /locus_tag="VNR65_11545"
     CDS             2708..3346
                     /locus_tag="VNR65_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HWJ19348.1"
                     /translation="MAWTREEMAARAAKEMRDGFYVNLGIGIPTLVANYIPANITVQL
                     QSENGMLGMGPFPYEGEEDADLINAGKQTITELPTTSYFSSADSFAMIRGGHIDLSIL
                     GAMQVAENGDLANWMIPGKMVKGMGGAMDLVAGVKKVVVVMEHVAKDGPKFLHRCNLP
                     LTGARVVDLLITDLAVFSIDKHHGGAALIEIAKNVTLDEIKAKTEADFTVAV"
     gene            complement(3568..4224)
                     /locus_tag="VNR65_11550"
     CDS             complement(3568..4224)
                     /locus_tag="VNR65_11550"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ19349.1"
                     /translation="MIPRLAAALRNDGVLPELLGMKKIVSEDAVRRAFKAIGADEGRD
                     WLQRHLDYCTAPLLSEPWILDADSTVKPLYGHQEGALLGYNPKKPGRPCHVYHTYTIA
                     GLRLVLDVDVAPGSEHASKHAAPGLWQLLDRIPRDCWPAFLRGDSGFGTEAIMSEAEQ
                     RGLPYLFKLRLTANVKKLIKKTFSKSGWADAGGANVYRERQALTYGWQGRHETLRLEG
                     "
     gene            complement(4229..>4716)
                     /locus_tag="VNR65_11555"
     CDS             complement(4229..>4716)
                     /locus_tag="VNR65_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ19350.1"
                     /translation="LLVSPLQPTEIMIGKAVPGLIIGVVLGTVITGIVTLVFGIPLTG
                     SIFVLYAGLIVFTLAIIGVGLFISSLVSTQQQAMLGAMVYMMPAMMLSGFTSPVQNMP
                     DWLQPVALLNPLTHFLVIVRGVFLRDMPMALVVERIWPMVVIAVFTLTAASWLFRRKL
                     G"
BASE COUNT         1032 a         1437 c         1381 g          866 t
ORIGIN      
        1 gaaccttgag atcaaggctc cctggcttca caattccatc aatgctcgca acggtgaatg
       61 gaaatgaact tccgcgaaga ccccagccga ggtcctgctc gagaagagcg gtgccaagtg
      121 tctgggccac gtcaccctcg agggagccgc ctgcggggaa ccggatcgaa aaggtccccg
      181 ctgcagcgct cacatcttcg gccggttctc tcatggtcac cggcatggtc aatgcggctt
      241 gaagcataac gggaccgctg aagccaaacg gcagctgaaa agcgcttgca agcgctttag
      301 cgtccagctg tgacccgctc gtgcgcaaga aaagcgaagc cggctggccc ggattgatat
      361 caagccttcc ggttgcatgt ccgccgaaaa ccgcgacgga ggcgatgtcg acgctcagcc
      421 gatcctggat gtgggtcaaa gcgagcgcca aaggtccggt ttcaaaggaa gccgcccgga
      481 aacggtcggc ggagacgcgt agatcgaaat cgaggggacg atccgtctcc cgccgggcaa
      541 gcggcagagc agcgagagga ggattttcgg cgaagcttcc gctgccggat atccaggccg
      601 gggtcacgtc caacgtgtcg tatgcgatcg tcccttcgag gcggggccga acgcccgtca
      661 tatccagagc gagggaacct ttggcggcat ggtctccgaa cgagattgcg gcgttatcga
      721 gcgctgcgcg ccttctcgac aaggtcaact ctccggacaa tgcgatcgtc tcaggccgct
      781 cccacggcgc cacggccgcc agaattcttg cccctccgcc cacgcccaaa gccgctctca
      841 ggcgaagcga cccgaacgcc gtctgacacg cgcctgcgca ttggccccga aaggatacct
      901 caaaccccct gctcgtcacg tccaaggcgt aaagtgcgct ctccgaccct ggtgcgttct
      961 tgacgaatac gcccgtcgaa atagaggaga cggcatcctt actgagcctc acccggctca
     1021 actcggctcg ctcgtaggcg ccgtttgcgc tcgcggtgaa gaacaacacg ggatcgcgca
     1081 attcgacgtc tgcgaaaggg cttcctgccg aaagagccat cgccagacgt gcggctccaa
     1141 gcccataagc cgtctgagcc ggttccggaa agccacggcg gaacacaacg tgcggtgaag
     1201 cgaccacgaa tttcttgaat tcgagcttgc cgcgaagaag cgaggaaaga cggacgacgg
     1261 ctgtgacgga cttggcccgc gcaatgcgaa tggggccctg ctccggcatg gatttgagag
     1321 cgatgccggt agcctcaacg gaaaggttgg cgaagctgcc gatccgaatc ggtcccgtga
     1381 tcgacaccgg gccgccgctc caggctgata agctctgcga gagcgtaccg cggatcgcct
     1441 cggcatcaac aacgacaggg agaatcacgg cgagtgcgcc tgccgcgata attaggctcc
     1501 ctgccagaat accggcccag cgtaaaacat atcgaattct acgggcgatc tgatgcatct
     1561 ttccttggac tatcgggatg tgaacagtcg cgctgaagcc gtcatgaatt tttgacaagc
     1621 tgcacccggt cgcccgcttt gttcctgtta aggttttagc gcatcgccag accttggcga
     1681 ctccgtacta ctgagcttgc acatgaaaac gacaccgctg tggacgcccg ctgtcaaaac
     1741 ggtggaaaac tcacgcatgg cccaatccat ggcggagatc aacacaactc agggacagag
     1801 ctgccggtcc tccacacgat ttcagaattg ccaatcagga gctttttggg cgaaggcatg
     1861 gaattcttca tgtcataagc gagcgcggcg acgggcccca tctcggacta cgccgaaaat
     1921 ttgctccgca agaggcctcg gtataatatc atttcctcga actgagggat ttcttgatga
     1981 aaaaagtatt tccggacgcg cacgcagcag tggctggact catcaaagac ggcatgacga
     2041 tcatgtccgg cggcttcggg ctttgcggca ttcccgagaa cttgatcgaa gcggtccgag
     2101 attcgggcgc gaaaaatctg acgatcatct cgaataattg cggcgttgat ggaattggcc
     2161 ttggcgtcct gctcgagaca agacagatca aaaagatgat ctcatcctat gtgggtgaga
     2221 ataagctttt tgcgcagcaa tttctttccg gcgagttgga gctggagttc actccgcagg
     2281 gtactttggc cgagcgcatc cgcgccggcg gcgcgggtat cccggccttc ttcaccaaga
     2341 ccggctacgg tacgatcatt gccgagggca aggaaacgcg cgagttcgac ggcgagcact
     2401 acgtcatgga acgcggactg accgccgaca tcgctctcgt gcacgcgttc aagggcgaca
     2461 acgaaggcaa tctcacctat cgcaagacgg cgcgcaattt caacccgatg atggcgactg
     2521 cagcgaagat cacgattgcc caggtcgaac atctgttcca cgctggcgag atcagtccgg
     2581 acgaggtcca cacgcctggc atttttgtgc aacgcctcat ccgggtatcc gacgcgaaaa
     2641 aacacatcga gcagcgaacc acgcgcaaac acacctcgcc cgcggcggca accggaggag
     2701 acctttaatg gcctggaccc gcgaagaaat ggccgcgcgc gccgcgaagg aaatgcgtga
     2761 tggcttctac gtcaatctcg gcatcggcat tccgacgctt gtcgctaatt atattcccgc
     2821 caacatcacc gtacagctgc agagtgagaa cgggatgctc ggcatgggcc ccttccccta
     2881 tgagggcgag gaggatgccg atctcatcaa tgccggcaag cagacgatca cggaattgcc
     2941 gacgacgagc tatttctcct cggcagattc ctttgcgatg atccgcggcg gccatatcga
     3001 cctttcgatc ctcggggcca tgcaggtcgc agaaaatggc gatctcgcca attggatgat
     3061 ccccggcaag atggtcaaag gcatgggcgg ggcgatggat ctcgtcgcgg gcgtcaagaa
     3121 ggtcgtggtg gtgatggagc atgtggccaa ggatggcccg aagtttttgc accgttgcaa
     3181 tctgccgctg actggtgcgc gtgtggttga cctgctgatc accgatctgg cggtattttc
     3241 gatcgacaag catcacggcg gcgcagcgct catcgagatt gcgaaaaacg tgacgcttga
     3301 cgagatcaag gcgaagaccg aggcagactt cacagttgcg gtgtaaccga gggggtcgtc
     3361 gtcccggaaa ggcgaagcct tgtccgggat cgttacacat tgaaatacct gctttttgcg
     3421 gtcccggctc gcgcttcgct tggcctaggc gtccgcgtcc gggccgattt caacgaagcc
     3481 aagcgccatc tggttttcac cgcgcccggc gatggtcagc ccctctttca gccggcggcg
     3541 caggatcaca acctggcgct ggcggcttca gccttccagc cgcagcgttt cgtgtctgcc
     3601 ttgccagcca taagtgagcg cttgtcgctc acgataaacg ttcgctcctc ccgcatcagc
     3661 ccagccgctt ttggaaaaag tcttcttgat cagcttcttg acgttggcgg tgagccgaag
     3721 cttgaacaga tagggcagcc cccgctgttc ggcctcgctc attatcgctt ccgtgccgaa
     3781 accgctatcg ccgcgcaaaa acgccggcca gcaatcgcgc gggatgcggt ccagaagctg
     3841 ccaaagccct ggcgccgcat gcttcgaagc atgctcgctt cccggagcaa catccacatc
     3901 gagcaccagg cgcaaaccgg cgatggtgta ggtgtggtag acatgacagg gccggcccgg
     3961 tttcttggga ttatagccaa gcagcgcgcc ttcctgatgg ccataaagcg gcttgaccgt
     4021 cgaatccgca tccagaatcc agggctcgga caaaagcggc gccgtgcagt aatccaaatg
     4081 acgctgcagc caatcccggc cttcgtccgc tccgatggcc ttgaaggcgc ggcgcactgc
     4141 atcctcgctc acgattttct tcatgcccaa aagctcaggc agcactccat cgttccgcag
     4201 cgctgctgca agccggggga tcaaggcctc accccagctt ccgccggaac agccagctcg
     4261 ccgccgtcag cgtgaacacc gcgatgacca ccatcggcca gatgcgctcc acgacaagcg
     4321 ccatcggcat gtcgcgcagg aacacgccgc gcacgatcac gaggaaatgc gtgagcgggt
     4381 tcaggagcgc cacgggttgc agccagtcgg gcatgttctg cacggggctc gtgaagcccg
     4441 acagcatcat ggctggcatc atgtagacca ttgcgccgag catcgcctgc tgctgcgtcg
     4501 agacgagcga cgagatgaac agccccacgc cgatgatcgc cagcgtaaag acgataagcc
     4561 ccgcataaag cacgaaaatc gagccggtga gcggaatgcc aaacactagc gtcacgatcc
     4621 ccgttatgac cgtcccgagc accacgccga tgattagccc cggcaccgct ttaccgatca
     4681 tgatttccgt cggctgcaaa ggcgagacga gaagct
//
LOCUS       DATMGX010000703         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_83821, whole genome shotgun sequence.
ACCESSION   DATMGX010000703 DATMGX010000000
VERSION     DATMGX010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83821"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..1337
                     /locus_tag="VNR65_11560"
     CDS             360..1337
                     /locus_tag="VNR65_11560"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ19351.1"
                     /translation="MHRREFIMLLGGAAAWPLTARAQQPERIRRVGVLTPKPDFVDRD
                     DVAAFTQEFKRLGWEEGRNFHLEYRAVAPDVEQLRSAAADLVGLKPEVILVVSSPVLR
                     VVQVNTDTIPIVFVGVTDPVDRGFVASLEHPGGNITGFASFEFSFGSKWLQLLKEISP
                     HLRHFAVIYHPGTTPGGWLPVIKSAAPVFGLEVNEAAVHDDKEIDGAIDALKTSSDAG
                     VIVLPSSFTMLHRQRIIDFAARYRLPAVYWDRRFVNDGGLMSYAYSLDEEERQGATYV
                     DRILRGEKTGDLPVQETTKFELVINLKTAKALGLTVPPTLLATTNMVIE"
     gene            1673..>2066
                     /locus_tag="VNR65_11565"
     CDS             1673..>2066
                     /locus_tag="VNR65_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19352.1"
                     /translation="MKKQRTMTTEPKRRNAPKVARRRGSVAGGLQEKLDLRTRELRAA
                     LEQQTAAADVLKVISRSAFDLNAVFETVAESSVTLCGADRAIIFRFDGEMLRAAAAFN
                     APQKLTDWLERNPIQPGRHSVAARAALEH"
BASE COUNT          426 a          604 c          607 g          429 t
ORIGIN      
        1 tacaatatga aacgcgtgat gaggattcta ggcgttggcg ggttgatgga ggcaatccac
       61 gcatgagaca ggcttcacag gcctcaataa acccgcgttc ttcgcctcaa ggagccaata
      121 agcgttcgca cggaccaatt ctgccgaatt ggccgcgccg agccgcacaa tcccgccggg
      181 atctctgctt acgccgccca caaacaacac cgaccgcgtt tttacacggc ctggacccat
      241 agcggacatc gcgcctactg aggaataggc gcacggttgt gtgccatttc tcgctcgccg
      301 cctcgtcgca aagtgctagg cttcagccat tgcaaaaggc gtgttctcag aggaggccga
      361 tgcaccggcg cgagttcatc atgcttctcg gcggcgcagc ggcgtggccg ctcacggcgc
      421 gtgcgcagca gcccgagcgg attcggcgcg tcggggtgct gacgcccaag ccggattttg
      481 ttgatcggga cgatgtcgct gcctttacac aggagttcaa gcgcttaggt tgggaagaag
      541 gccgaaattt ccaccttgaa taccgtgcgg tggcacccga cgtggagcaa ctgcgatccg
      601 ccgccgcgga cctggtgggc ctgaagccgg aagtaatcct tgtcgtgagt tcgccggtat
      661 tgagggtcgt gcaagttaac accgatacga tccccatagt cttcgtcggc gttaccgacc
      721 ccgtggaccg aggctttgtt gcgagcttgg agcatccagg cggcaacatc acgggtttcg
      781 cctcgttcga attctccttc gggtcgaagt ggctccaatt gctcaaggag atttcgcccc
      841 atctccgcca cttcgcggtc atttatcatc ctggaaccac gcctggaggc tggttgcccg
      901 tgatcaaatc ggctgctcca gtctttggtc ttgaagtgaa tgaagctgcg gtccacgacg
      961 acaaagaaat cgacggcgcc atcgatgcgc tcaaaacatc ttcggatgcc ggcgtgattg
     1021 ttctgccaag ttctttcaca atgctgcatc gccagcggat catcgatttt gccgcacgct
     1081 atcggctccc agcggtctat tgggataggc gtttcgtcaa tgatggtggc cttatgtcct
     1141 atgcctacag tctggatgaa gaggagcgcc agggcgccac gtatgtcgac cgcattctta
     1201 ggggcgagaa gacgggcgat ctccctgtcc aagagacgac aaaattcgag ttggtcatca
     1261 acctcaagac cgctaaggcg ctcggcctca cggtgccgcc gacactgctc gccaccacca
     1321 acatggtgat cgaatagaga tgctgtttgc tgcggtgcgt cggtcgctat tggcacttaa
     1381 cggacaaacc gcccgcgccc gagtttgtcg cctattggac taacaacgga caaagggcgg
     1441 cgctggggtt ggatatctcg gcagcgattg acccataagc gacattggct gtgcactgcg
     1501 gcaatggttt tgatgccggt ttcagcccct ttcaaagtac ccgtttaaac cgatacgatg
     1561 tctagtccta agctaggggg gcaggcatga ggtggcggga agttattgcg ctgcctggtg
     1621 gaccgagagc ggccttttcg atcacttcaa gcctgcgaca accggcgaag caatgaaaaa
     1681 gcaacgcacc atgacgacag agcccaagcg ccgcaatgcg ccgaaggtcg ctcgtcgtcg
     1741 cggttctgta gcaggtggct tgcaagaaaa actcgacctt cgcacccgcg agctccgcgc
     1801 agctctggag cagcagaccg ccgctgccga cgtgctcaag gtcatcagcc gctcggcgtt
     1861 cgacctcaac gcggtgtttg aaactgtagc cgaaagttca gtcacgttgt gtggggccga
     1921 cagggcgatc attttccgtt tcgatggcga gatgctgcgg gcggcggcgg cgttcaacgc
     1981 tccccagaaa ctaacggatt ggcttgaacg aaatccaatt caacccggcc ggcacagcgt
     2041 tgccgcccgt gccgcgctcg aacacc
//
LOCUS       DATMGX010000704         2905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_2928,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000704 DATMGX010000000
VERSION     DATMGX010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VNR65_11570"
     CDS             <1..414
                     /locus_tag="VNR65_11570"
                     /inference="COORDINATES: protein motif:HMM:NF025473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HWJ19353.1"
                     /translation="LFSFANALALTAWLGLLFLPHVAAIRNVIAAAVIPCLFAAAYVV
                     ILARFFEPGGFAKFSTLEGIASLQSSPWLLLAGWLHYLAFDLFVGAWEVRTARRDGIA
                     HLAIVPSLILTFIVGPAGLLAFTVTRLLLRKRQGG"
     gene            476..1033
                     /locus_tag="VNR65_11575"
     CDS             476..1033
                     /locus_tag="VNR65_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19354.1"
                     /translation="MTLAREFWFSFFIFLPNFLMAQFVMAADQVQSSKVDDRATFLDG
                     EWTGTGSFFLGKDWGNQIMSCSEVKLKYVGTKTKYEVHEGFAVCNGKLNPFNDDSKFS
                     IKEDGTIFYVSSKDHTLIPDTIAGSVRDGTLHTATVHGNSTDDVTIMRKGDILIYRQW
                     HAASEGNPAYVFTAILTQDPSLIKK"
     gene            1239..1625
                     /locus_tag="VNR65_11580"
     CDS             1239..1625
                     /locus_tag="VNR65_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ19355.1"
                     /translation="MRLGYVIIYVSDLEATVVFYEKAFGIVRRFIHESGYAEMDTGAT
                     ALAFASETLGQTNGMAYRPNRLKDDAAGAEIAFVTDDVAGAYAKAVAAGASAYKEPTK
                     KPWGQTVAYVRDNNGFLVEICSPIVA"
     gene            1634..1972
                     /locus_tag="VNR65_11585"
     CDS             1634..1972
                     /locus_tag="VNR65_11585"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWJ19356.1"
                     /translation="MVSRQSTVDFIVEQMAAAGDVSAKKMFGEYAIYCDGKLVALVCD
                     GQLYIKPTISGRSFIRNCVEAPPYKGAKPSLLISGDQIEDAQWLSKLVGISAAELPQP
                     KKNKRRKRKD"
     gene            complement(2163..>2905)
                     /locus_tag="VNR65_11590"
     CDS             complement(2163..>2905)
                     /locus_tag="VNR65_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HWJ19357.1"
                     /translation="TKPFFGMGDPIFGNSSASPDSARGIKRSSPPLAAIYRNGVADLR
                     ALAGLVPLPETAGELQAVAQTLNAPDEDVNLREAATETRVKSAPLKDYRILHFATHGL
                     VAGDLTGLNEPALVLTLPRVITEADDGLLTASEVATLQLNADWVVLSACNTASGDKVG
                     ADALSGLARAFFYAGARALLVSHWAVDSQATVDLTTRTFAALAADPRLTRAEAFQNAI
                     LMAIDEGAPPNYWAPFVIVGEGGGGLSR"
BASE COUNT          635 a          781 c          837 g          652 t
ORIGIN      
        1 cttttttcgt tcgccaacgc tctcgccctc accgcatggt tggggctcct gttcctgcct
       61 catgtcgcgg cgatcaggaa cgtcattgcc gccgctgtga tcccttgcct attcgcggca
      121 gcgtatgtcg tgatcctggc ccgttttttc gagccagggg gcttcgccaa attctctaca
      181 cttgagggga tcgcttcact tcagagtagc ccgtggctgt tgctggcggg ctggttgcat
      241 tatttagcct tcgatctgtt tgtaggagct tgggaggtcc gcaccgcgcg gagggacggg
      301 atcgcacatc ttgctatcgt tccgagcctc attctgacgt tcattgttgg gccagcgggg
      361 cttctggctt ttaccgttac ccgcctgctc ctccgcaaac ggcagggcgg ctagaaatgg
      421 gagatcgaag gaagcgatcg ctcagcttcc aggcaaatac gatggagcag acagtatgac
      481 tctcgctcgg gaattttggt tctctttctt tatcttcttg cccaactttc ttatggctca
      541 attcgtgatg gccgccgatc aagtacagtc ttcgaaagtt gacgatcgag ccacgtttct
      601 cgatggcgaa tggactggca cgggttcatt ctttttggga aaggattggg gaaaccaaat
      661 catgagctgt agcgaagtaa agttgaaata tgttggaact aaaacgaagt atgaggtcca
      721 cgaaggattt gcggtttgca atggtaagtt aaatccattc aatgatgact caaaattcag
      781 catcaaagag gacggaacaa tcttctatgt aagttcaaag gatcatacgt tgatccctga
      841 tacaatcgcc gggagcgtgc gcgacggcac tctccacacg gcgaccgtcc atggaaactc
      901 gacagatgat gtaacgataa tgagaaaagg ggatattttg atttatcggc aatggcatgc
      961 ggcttcggaa ggcaacccgg cctatgtttt taccgcgatt ttgacgcaag acccatccct
     1021 gattaaaaag taaccaggtt cttcgcggtt ccaagctctc ctcttcggcc ccctccggtt
     1081 atatctcttg aaggccgggt gtgggctaaa gcggcctcgt ccccaatcga tggtagagaa
     1141 gaatcgaagg tagaatagag acttagtccg agtcggagag gcttcgcacg aagagcgaac
     1201 cttgatatgg tcaagcgatg aaattcgagg agggatcaat gcggttgggc tatgtcatca
     1261 tctacgtttc tgatcttgaa gccacggtcg tgttttacga aaaggcgttc ggcatcgtcc
     1321 gccggttcat ccacgagagc ggctatgccg agatggatac gggcgcaact gcgctggcct
     1381 ttgcatcgga aacgctcggg cagactaacg gcatggccta tcgtcccaac cgattgaagg
     1441 acgatgccgc tggagccgag atcgcgtttg tcaccgacga cgtggcaggc gcctatgcga
     1501 aagccgtcgc ggcaggcgca tcggcttata aggagccaac gaaaaagcct tgggggcaaa
     1561 cagtcgccta cgtccgggac aataatggat ttctcgtgga aatctgctcg cccattgttg
     1621 cttgacaacg tttatggtct cgcgacaaag caccgttgat tttatcgttg aacagatggc
     1681 cgctgccgga gacgtgtcgg ccaagaagat gttcggtgaa tacgcgattt attgtgatgg
     1741 aaaactggtc gcccttgtgt gcgacggcca gctctatatc aagccaacga tatctggtcg
     1801 cagcttcatt cgaaattgcg tcgaagcacc gccttataaa ggggcgaagc cgagcttatt
     1861 gatctcaggc gaccaaatcg aggacgcaca atggttgtca aagctcgtcg gcatctctgc
     1921 tgcggagctg ccgcagccaa agaaaaacaa acgccgcaaa cgcaaagact gaccccaagg
     1981 ccgatgcctc ttacggcaga agtgggtgat ttcgccagcg gattacagcg ttcaaaccct
     2041 gtcgatttcg tccgctcttg atgggcttgc tgccttaagt tcgaatttat cgaaggaccg
     2101 ctcgcgaccc gactcgccct tgtggctgtg agccgaagta tcaaaaactt gtgcttgatg
     2161 cgtcagcggc tcagcccgcc cccaccttct ccgacgatca cgaaaggcgc ccagtaattg
     2221 ggcggagcgc cttcgtcgat cgccatcaga atagcgtttt ggaaggcttc ggcccgcgtc
     2281 aatcttgggt cggcggcgag cgcggcgaaa gtccgcgttg tgagatccac agtggcttgc
     2341 gaatccacgg cccaatgcga gaccagaagt gcgcgtgcgc cagcatagaa gaaagcgcgc
     2401 gcgaggccgg acagcgcgtc cgcgccaacc ttgtctcccg aagcggtgtt gcaggccgaa
     2461 agcacgaccc aatccgcgtt taactgcagc gtcgccacct cggatgcggt caagaggcca
     2521 tcgtcggctt cggttatgac gcggggcagc gtcagcacga gcgccggttc gttgaggccg
     2581 gtcaggtcgc ccgccacgag gccatgcgtg gcgaaatgca aaatgcggta atccttcagc
     2641 ggcgccgatt tcacccgcgt ttcggtcgct gcctccctga gattgacgtc ctcgtccggc
     2701 gcattgagcg tctgggcaac ggcctgcagt tcacccgccg tttctggcaa gggcacaagt
     2761 cccgccagcg cgcgcaagtc cgcaacgccg ttgcgataaa tggcggccag cggaggggaa
     2821 ctgcgtttta tgcccctggc gctatcgggt gaagcggacg aattgccgaa aatgggatcg
     2881 cccatgccga agaaaggctt cgtcg
//
LOCUS       DATMGX010000705         4001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_257106, whole genome shotgun sequence.
ACCESSION   DATMGX010000705 DATMGX010000000
VERSION     DATMGX010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257106"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VNR65_11595"
     CDS             <1..737
                     /locus_tag="VNR65_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19358.1"
                     /translation="QFNEVFWDEDSGFYALALDGEKRKVLTVASNPGHCLLSGIVPAD
                     RARKVVERLMAPDMWSGWGIRTLSTLNPAFNPYNYQTGSVWPHDNGLIALGFKRYGFG
                     AEAARIARDISEAGTHFLLNRLPELYTGPPRDESTFPVQYLGANVPQAWAAGSVFSLM
                     QAMLGFMPDASQGKLYIDPLLPRWLPDLTVLDLRVGEHVFDISFWREGEDTKFEVLRG
                     DPQAVERSSFVVQSERLMHDALNLEL"
     gene            complement(1956..2327)
                     /locus_tag="VNR65_11600"
     CDS             complement(1956..2327)
                     /locus_tag="VNR65_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19359.1"
                     /translation="MSDKSLVDERGEPMKDPVDQFMRQLASSAAKHRRTVTGAPPVIV
                     ENALATKNEPARMPEIWKTEERLAPAIESVSVAEAEEVPGCWVVDACDSDNALLRAVF
                     DGPDAEQRAREYARWKYGADV"
     gene            2540..2743
                     /locus_tag="VNR65_11605"
     CDS             2540..2743
                     /locus_tag="VNR65_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19360.1"
                     /translation="MDKEIYLKLIVLGAVLILALLSPIVSALALTGIVAWTIRDKKIG
                     EIPLVKGLVEVAPPSVKAFFNPK"
     gene            complement(3170..3625)
                     /locus_tag="VNR65_11610"
     CDS             complement(3170..3625)
                     /locus_tag="VNR65_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HWJ19361.1"
                     /translation="MNKESDETEQPGEDDEVTLSISPEQVCFIIVKAREFDVKDAVSE
                     PDPGSNPSDDKDVAVLEDHEDDPVVEELTSFIDAMSEDAQIDLVALAWLGREDSTAKD
                     WEGVREEAARAHNARTAAYLLGMPLLGDFLEEGLSMLGYSCAEFEVGRL"
BASE COUNT          860 a         1124 c         1076 g          941 t
ORIGIN      
        1 agcaattcaa tgaggttttc tgggacgaag actccgggtt ttacgccctt gccctcgatg
       61 gcgagaagag gaaggtgctg acggtcgcgt ccaaccctgg tcactgtctc ttgtcgggca
      121 tcgtgcccgc cgaccgcgcc cgcaaagtgg tcgaacggct gatggcgccc gacatgtgga
      181 gcggatgggg catacgcacg ctttcgacgc taaatccggc gttcaatcca tacaattacc
      241 agacaggctc ggtctggcca cacgacaacg gcctcatagc gcttgggttc aaacgctatg
      301 gctttggcgc cgaagctgcg cggatcgcgc gggacatcag cgaagccggc acccattttc
      361 tgctgaatag gctccctgaa ctctataccg gacctccgag ggacgagagc acattccccg
      421 tccagtatct cggcgccaac gtacctcagg catgggcggc agggtcggtc ttcagcctga
      481 tgcaagccat gctgggcttt atgccggatg cgtcacaggg caagctttac atcgacccgt
      541 tgctgccgcg ctggctgccc gatctcactg tgttggacct tcgcgtggga gaacatgtgt
      601 tcgatatcag tttctggcgc gagggcgaag acacgaaatt cgaggtgctg cggggcgatc
      661 cgcaagccgt cgagcgctcc agcttcgttg tgcaaagcga gcgcctgatg cacgatgcgc
      721 ttaacttgga gctgtgactc cgggttcgcc aacatttgga tttgcgagtt ttcaggacca
      781 cacccgcgct ggcccacgat gaattttata aaaaccgcgc cttaaaggcg cggttgagca
      841 cggtcctcaa tgccgtttga gctgcaccag tacatagcag gaacggaatg aaggcaggag
      901 ttatcccagt tgcgtgtctt tctttggcta tcgtgatccg gattgtagta ggagggctcg
      961 ctttgaattt gaccttcccg actgaactcc ggtcttgcat cagatgtcgc cacacagaat
     1021 agcgtcagca aaataccgcc cgatattgta acaaagatct tcatagttat tccctccagg
     1081 cgatgtgcca ggattagaga ccaggagtgc acgtattagt cgcgcgccat gttcggatcg
     1141 gtcttaaaaa accgcgcctt taaaggcgcg gtttaatacg gctctcaacc gccgtttgaa
     1201 ctgcatgcat attgatcgga aaggccggta gcacgactga agcaggaact atcccggtcg
     1261 cgcgcggtat cgtaacccga gcgatagtag gaggactcgc tttgagttag agcttgccgt
     1321 tgggcatgct gtctagcatc cgacgacgtt gccgacgccg ccacaagcag catgcccgct
     1381 gaaaacgtca cgaacgtctt ggaaaggttg agagacatga tgatttcctc agattaggaa
     1441 gtcgatccat tcgagcttct tcgaaatgag tagaccatcg cttcgctatg gactgttccc
     1501 taggtaacaa tgtcgaagta ttgtgtgtcg ttttctttgc tacgcctaag cttagaacgt
     1561 gatttttcta atccgagttc agcaaatttt gagaatttcc tatattttat aagataataa
     1621 gcggcgcacc gggataagcc cctggtagct tcacgaggaa attgtaaaat gtcttcaact
     1681 tctaatgacg ctctgatccg tggaaggtgc aggcgcaaga gccaaagaag caacgaagag
     1741 gacgcgaagc cgctgtggca cgcctttcga cttcgccgga ttactcaaag gtgctggctc
     1801 cggttcgaaa agtgcttaca tgcttgcttg ctgaaggtgt ttttgccaaa caaacccaat
     1861 tttaaccggt tgtacaccct cgcagtgact ccggattaag cttgaacctg ctcgcctatc
     1921 agtgatgcag ccacgatgct ctgaacgcca tcgcgctaaa catcagctcc gtatttccag
     1981 cgggcgtatt cgcgtgcccg ctgctccgcg tccgggccgt cgaaaaccgc gcgaaggagt
     2041 gcgttgtcgc tatcgcacgc gtcgacgacc cagcatcccg gcacttcctc cgcttctgca
     2101 accgagacgc tttctatcgc gggcgccaat cgctcttccg tcttccagat ctccggcatc
     2161 cgcgcaggtt cgttcttcgt cgccaaagca ttttcaacaa taaccggcgg ggcgccggta
     2221 acggttcgcc tatgctttgc ggcgctggag gcaagctgcc gcatgaactg atccaccgga
     2281 tccttcattg gttcgccgcg ttcgtcgacc aaagacttgt cgctcattcc gattccctct
     2341 cagcagcgga cgaagacgca cgctagcagg agccgaaagc cgtggaaagc cggggctcac
     2401 gcctatcgac aggataagcg ccggggtaaa atgtttccgt ttgccttgaa tcgttttatt
     2461 tgctgaatcg ttctatgagt gttcccatgc agtcgatcgc tccggttttc gaccgcgctt
     2521 catatggagg caaaatcgaa tggacaaaga aatatacctc aagctcattg tcctgggcgc
     2581 ggttttgatc ttggcgcttc tgtccccgat tgtctcggcg cttgccttaa ccggcatcgt
     2641 cgcctggacc atacgagaca agaaaatcgg cgagatcccg ctggtaaagg gactggtcga
     2701 agtcgctccc ccgagcgtca aggcgttttt taatcccaaa tagagccaga ttcgttcatg
     2761 cgcggcagaa gtgccgttct tgttgtggat cgctcactct caacgcattc gacggacaaa
     2821 ctttttccgt tgagccttgc taatgagggt tcagcaacgg gtggcgaaag atttctcgga
     2881 tgcgctttgt agtcttggtt tggttgctct tgtcaggctg ctccctgcag cgcgcctccg
     2941 agcagccgga cgcttccatc tcctttccaa aacctgtggc ggaggccgct cccaagaaag
     3001 aggaaagctg cgtgcctagc tccacattgc tagcgatggt ggagtgcctc gcaaaatagc
     3061 cgccgctcgg cgccatgttc cgccaaacgg ctgcggcccg cactaagcta gcgttcgctg
     3121 ccttccatcc gtcaggccgt tcgccctgag cgcctctgcg tgaactctgt cataggcggc
     3181 caacttcgaa ttccgcgcaa gaatagccga gcatcgagag cccttcctcc aggaaatcgc
     3241 cgagaagcgg cataccgaga aggtaggcgg ctgtcctcgc attatgggcg cgggctgcct
     3301 cttcgcgcac gccttcccaa tctttggcgg tcgagtcctc gcgtccaagc caagcgagtg
     3361 caacaaggtc aatctgtgca tcctccgaca tggcatcgat gaaggatgtg agttcttcga
     3421 cgacggggtc gtcttcgtgg tcttccagca ccgccacgtc tttatcgtcc gacggattcg
     3481 atccaggatc cggctcgctc acagcgtcct tgacgtcgaa ttcccgcgcc ttgacgataa
     3541 tgaagcacac ctgctccggc gagatcgaga gagtgacttc atcgtcctcc cccggctgtt
     3601 ccgtttcgtc tgactctttg ttcatgatta ccacctttgg atcctgccgg cgcatcaaaa
     3661 aatggccaaa cggatgacgg tttaccaaaa aataaagccc ccgaaggcac ttgataagag
     3721 gtgtcggctt tcatgttttc cgccaagcca ctcttggacg tgtaagacac tgctatttta
     3781 agttggcgca gaaacactac cgttgaacac aaatctgcag aagcgtcaca atatcggatg
     3841 acgcagccgt tcggggtatt cctgttacaa tcaaggcacg cgttttccct tgttccgtca
     3901 ccttgtgctt tcaattttcc gatatcgagg aagggaggcg gaaacggaga tagcatatga
     3961 aaagaacgac tttcctggcc cttgccatga gtttaggctt g
//
LOCUS       DATMGX010000706         2490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_300692, whole genome shotgun sequence.
ACCESSION   DATMGX010000706 DATMGX010000000
VERSION     DATMGX010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300692"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VNR65_11615"
     CDS             <1..670
                     /locus_tag="VNR65_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19362.1"
                     /translation="DYAFTALACLDAYEVTGAMSYFKFAQQIADEMVRRFGDATSGGF
                     FDTEATLEDKLKLGALSARRKPFQDAPTPAGNPSAAIALLRLHAYTNDPRYRDKAEDT
                     LQVFAAMAEQFGIYAGTYGIAATWMSRPHTQAVIVGEDQKAAELLRAAAAPFAVNKSV
                     VHLTDSEAVAQNLPPVLAETIPNLPALKAKPSVAIVCTNFACRPPVDSAEELSAMLRD
                     ALAG"
     gene            964..1545
                     /locus_tag="VNR65_11620"
     CDS             964..1545
                     /locus_tag="VNR65_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19363.1"
                     /translation="MPRKLKNKTSSKTISAVKKLRNSLIARSAAKKSSAKTPKKNSTS
                     PKTSSGKRAGGFHGGNYVTPKGNVSIGIPAFWTLRQTNDDLEVESPSCKTSVIVTAFQ
                     RENGVAKLDAREYLEHFLQSASMKGRAHAGKSGPSRAQSRFRDPEGDHWQIEVLTNGD
                     TLLLATLNSSLPPRSQEPRVGTEILGTITLNGK"
     gene            1790..>2490
                     /locus_tag="VNR65_11625"
     CDS             1790..>2490
                     /locus_tag="VNR65_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWJ19364.1"
                     /translation="MDPSLIRNFAIIAHIDHGKSTLADRLLELTGSLTAREMQAQVLD
                     AMDLERERGITIKAHSVRMMYPAADGNIYQLNLIDTPGHVDFSYEVSRSLASCEGALL
                     VVDASQGVEAQTLANAYIAINNGLEIIPVINKIDLPSADIERTKEMIESAVGLDASHS
                     LAISAKTGVGVPEVLEAVVQRIPPPKGRASNQLQALIFDSWFDSYRGVVVLARIIQGT
                     LYKGQRIRLMSNGRT"
BASE COUNT          558 a          731 c          745 g          456 t
ORIGIN      
        1 cgattacgct ttcaccgcgc tggcgtgtct cgatgcctat gaagtcaccg gcgcgatgag
       61 ctacttcaaa ttcgcgcaac agatcgccga cgaaatggtc cggcgttttg gcgatgcgac
      121 ctcggggggc ttcttcgata ccgaagccac gctggaagac aagctgaagc tgggagcgtt
      181 gtcggcgcgg cgcaagccct ttcaggatgc gcccactcca gccggcaatc ccagcgcggc
      241 gattgcgttg ttgcgcttgc acgcgtacac caacgatccg agatatcgcg acaaagcgga
      301 agacacgctc caggtttttg ccgcaatggc ggagcagttc ggcatttacg ccgggaccta
      361 tggcattgcg gcgacctgga tgtcgcggcc gcacacccag gcggtcatcg tcggcgaaga
      421 ccagaaggcg gcagagcttc tcagggccgc agccgcgccg ttcgcggtca acaaatcggt
      481 ggttcacctg accgacagcg aagccgttgc tcagaacttg ccgccagtgc tggcggagac
      541 catccccaat cttcctgcgt tgaaggcgaa accgtcggtg gcgattgtgt gcacgaattt
      601 tgcctgccga ccgccggtcg acagcgcgga ggaattgtca gccatgttac gggatgcgtt
      661 ggccggatag taagggcctt tagaggccgt gccccttcaa agcgtgagat catgatgaat
      721 aatgcaggtt cagcgctggc tcaagtggga aaagaggccg agtcccgtaa gggacgacag
      781 tgagatccga aatgaagctt tgcagaaggt tgccgataaa gaaaacagcg atgacaaccc
      841 gccagccaga gggcggggta ggacagccca cggctgagcc aaagcgagcg ggcggcacga
      901 aactgtgtcg cccgtgaaga tcaacgagca tctacatacc atcccaacct caggacaacc
      961 atcttgcctc gcaaactaaa aaacaagaca tcctccaaaa cgattagcgc tgttaagaaa
     1021 ttgcgcaact ctttaatcgc tcgcagcgct gccaaaaaga gctccgccaa gacgccgaag
     1081 aagaattcca cttctccgaa aaccagcagc ggaaaaagag ccggcggctt tcacggcggc
     1141 aactacgtca cacccaaagg caacgtgtcc atcggaatcc ccgccttctg gacgctgcgg
     1201 cagaccaacg atgacctgga agtggaaagt ccgtcgtgca agacctcggt gatcgtgacg
     1261 gcgtttcagc gggagaacgg cgtggcaaaa ctcgatgcgc gcgaatacct ggagcacttc
     1321 ctccagagcg cgtccatgaa agggcgggcc catgccggga agagcggtcc ctcgcgcgcc
     1381 caatcgcgct tccgcgatcc cgaaggcgat cactggcaga tcgaggtcct caccaacgga
     1441 gacacattgc tgctggccac cttgaacagc agcctgccac cgcgctcgca agagccgcgg
     1501 gtgggcactg aaattctggg gaccattacg ctcaatggga agtagctgcg cgcgaagcaa
     1561 ggcggctatg tctggggctt tattcttgcg ccgagatggg attcaaaacg cgcgcgtgat
     1621 gtaggcatca tgagcggaat ccaactgcaa tgatagcagg cacaaacagc gtgaaaattg
     1681 ctgtcgcgat tgcgctgacc gctgtgcgcg tgactaaact tcgtactccg cgccctccgt
     1741 ggggcgctgt tttctcctgt agaatcgagg tagcgctcag ctatctcgca tggatccatc
     1801 actcatccga aacttcgcga tcatcgccca tatcgatcat gggaagagca cgcttgccga
     1861 ccgcctgctc gagcttaccg gctcgctgac cgcgcgggag atgcaggcgc aggtacttga
     1921 cgccatggac ctggagcgcg agcgcggcat caccatcaag gcgcattcgg tgcgcatgat
     1981 gtatcccgcg gccgatggaa atatttatca gctcaatttg atcgacacgc ccggccacgt
     2041 ggactttagc tatgaagtgt cgcgttcgct ggcctcttgc gaaggtgctt tgctggtggt
     2101 ggatgcctcg cagggcgtgg aagcgcaaac cctggcgaac gcctacatcg ccatcaataa
     2161 cgggctggag atcatccccg tcatcaacaa gattgatttg cccagtgccg acatcgagcg
     2221 caccaaggag atgatcgaga gcgcggtcgg cctggatgcc tcgcactcgc tggccatcag
     2281 cgccaagacg ggcgtgggag tgcccgaggt gctggaggcc gtggtgcagc gcattcctcc
     2341 acccaagggg cgggccagca atcaattgca ggcgctcatc ttcgattcct ggttcgactc
     2401 ctatcgcggt gtggtggtgc tggcgcgcat catccaaggc acgctataca agggccaacg
     2461 cattcgtctg atgtcgaacg gccgtacctt
//
LOCUS       DATMGX010000707         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_281336, whole genome shotgun sequence.
ACCESSION   DATMGX010000707 DATMGX010000000
VERSION     DATMGX010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281336"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1123
                     /locus_tag="VNR65_11630"
                     /pseudo
     CDS             1..1123
                     /locus_tag="VNR65_11630"
                     /inference="COORDINATES: protein motif:HMM:NF008979.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
     gene            1248..>2098
                     /locus_tag="VNR65_11635"
     CDS             1248..>2098
                     /locus_tag="VNR65_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ19365.1"
                     /translation="MTVKAAREPMAFAPFEWLLAFRYLRARRKKGGVSVIAFFCVLGI
                     ALGVATLIIVLSVMNGFRKELFDKILGLNGHVIIRPLARPFNDFVDTADRLNAVPGVK
                     FSLPVVEGQVMVSSQSNSSGIIVRGLRTQDLDHLKAISGNIRYGSLANFDQSGGIVIG
                     TRLANSLNAFVGGEITVLAPRGATTPFGTAPRVKRYPITAIFEMGMSEYDGTIAFMPL
                     KEAQSFFNVGDAVHVLEVVVDNPDNVDAMRASLQDAAGPDLIMNDWRQRNATFYNTLK
                     VERNVMF"
BASE COUNT          426 a          620 c          647 g          405 t
ORIGIN      
        1 tggatttcgc gttctgaaaa agatcgagca gatcgttcgc gaggagcaga accgggctgg
       61 cgccatcgag ctgttgatgc ccacgattca gtccgcggag atctggcagg aaagcgggcg
      121 ctatggcgat tacggcgcgg agatgctgcg catcaaggac cgtcacgagc gcgacatgct
      181 tttcgggccg acgaacgagg agcagatcac gcaaatcttc cgcgatgccg tgaagtccta
      241 tcgcgacctg ccgaaaaacc tgtatcacat tcaatggaag ttccgcgacg aggtgcgccc
      301 gcggttcggc gtcatgcgcg ggcgcgagtt cctgatgaag gacgcctatt ccttcgatct
      361 cgacgccgat agcgcgcgcc aatcctatta ccgcatgttc gtcgcctact tgcggacctt
      421 cgcgcgcatg ggtctgaagg ccgtgccgat gaaggccgat accggtccga tcggcggcga
      481 cctcagccac gagttcatca tcctcgccga tacgggcgag tcggaagtct tctgcgaccg
      541 cgatctcatc gagatgcccg tcccggatga gacgacgaat tacgagcagg atctgcgctc
      601 cgtggtcgag cgctggactt ccaaatatgc ggcgacgagc gagatgcacg atgccgaccg
      661 cttcgagcgc gacgtgccgc aggaacggcg gctttcggcg cgcggcatcg aggtgggaca
      721 catcttctac ttcggcacaa aatacaccgc gaagatgaag tgtcaggttc agggcccgga
      781 tgggcagctc atcacggtgc aaggcgggtc ttacggcgtt ggcgtgtcgc gcctcgtcgc
      841 cgccatcatc gaagcgagcc acgacaaggc aggcatcatc tggccggagc aagttgcgcc
      901 tttcctcgtt ggtttgatca atctgaagcc cggcgacagc gcgtgcgacg cggcctgcgc
      961 gaagctttac accgctcttg gcggcgagaa cgacgatgtc ctctacgacg atacagggga
     1021 gagcgcgggc gcgaaattca agaccatgga cctgattggc ctgccttggc aggtcatcgt
     1081 ggggccgaag gggttggcga acggcgagat cgaagtgaag caccgcgcaa gcggggaaag
     1141 aaacacgttc gcccttgatg cggccataaa taagctgcaa cagaccgtga ccggggatgc
     1201 aggcgcgccg ccgaaaccca gaagctagcc caggacgcga tcagcggatg acagtcaaag
     1261 ccgcaaggga accgatggcg tttgcgccat tcgaatggct gcttgccttt cgctaccttc
     1321 gcgctcggcg caagaaaggc ggcgtatcgg ttatcgcctt cttctgcgtg ctcggcattg
     1381 ctctcggcgt cgccaccctg attatcgtgc tctcggtcat gaacggtttt cgcaaggagc
     1441 ttttcgacaa gatcctcggg ctcaacggcc acgtgattat ccggccgctc gcgcgcccct
     1501 tcaacgattt cgtcgacacc gctgaccggc tcaatgccgt gcctggcgtc aagttctctc
     1561 tgccggtggt cgaagggcag gtgatggttt cttcgcaatc caactcctcc ggcatcatcg
     1621 tgcgcggctt gagaacgcag gacttggacc acctgaaagc catctccggc aatatccgct
     1681 acggatctct cgccaacttc gatcagtctg gcgggatcgt gatcggaacg cggctcgcca
     1741 attcgctgaa tgcctttgtg ggcggggaga taacggtgct cgctccgcgc ggcgcaacga
     1801 cgccatttgg aactgcgcca cgtgtgaagc ggtatccgat aaccgcgatt ttcgaaatgg
     1861 gcatgtccga gtatgacgga acgatagcct tcatgccgct gaaggaggcc cagtccttct
     1921 tcaatgttgg cgatgccgtc catgttctgg aggttgtcgt cgacaacccg gacaatgtcg
     1981 acgccatgcg ggccagcctc caggatgccg ccggacccga tctcattatg aacgactggc
     2041 gacagcgaaa cgcgaccttc tacaacacgc tgaaggtcga gcggaacgtg atgttcat
//
LOCUS       DATMGX010000708         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_287220, whole genome shotgun sequence.
ACCESSION   DATMGX010000708 DATMGX010000000
VERSION     DATMGX010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287220"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VNR65_11640"
     CDS             complement(<1..500)
                     /locus_tag="VNR65_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HWJ19366.1"
                     /translation="MERAAELLMPAGSLEKLKVAILYGADAVYMGTPDLSLRTRSNFS
                     LDEVLEGVAFAHAHGKRAYLTLNLFSHNRDIEKLPEYVQTIREVRPDGVIVADPGVFN
                     FVKRAAPELELHISTQANVCSWLSVQFWGELGASLIVLAREVSFEELKEIRERCPDIK
                     LEAFIHG"
     gene            704..1294
                     /locus_tag="VNR65_11645"
     CDS             704..1294
                     /locus_tag="VNR65_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19367.1"
                     /translation="MKIEIKADKDAVLGGPDHIPYLKLELREDSYAVLHASHRASGRT
                     ASPMEEQSERICAWSASLDPGSYAIPDLAAIEALADRLHPLLARVAAWHSMDWDGSNY
                     ASEASAEVEKLLCAAQWWDREHQVWDADDWLFELGYLGAAKDYDLGVDSNEAAYKAAG
                     KKIEADALRYGVVLTNTDAVLRRIRQALQAEAKEDA"
     gene            1388..2290
                     /gene="rfbD"
                     /locus_tag="VNR65_11650"
     CDS             1388..2290
                     /gene="rfbD"
                     /locus_tag="VNR65_11650"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HWJ19368.1"
                     /translation="MRILAAGWHGQIASAFMQIAPGRKDISAFAIGRPGLDICEPRSV
                     ERALGDIRPDVLINLAGYTDVDGAESEPELALALNSAGARLLAEAAARRDVPIIHIST
                     SYVFNGHKKSAYVETDQTAPSTIYGQSKLAGEVEVQKANSKHIILRTEWLHSPFGRCF
                     VSNILQRAQLGMPLKVVNDQYGNPTYAPHLVDAILAVAAHLTSSPSDAIPWGVYHAAG
                     TGTATWYDVAREVLIKSEQLRGLSFSLEAITSAEYLTRSHRSPNAQLDCSKLKQTFGV
                     ELPDWQEGVHECVGRLLAPDLVAV"
     gene            2376..2750
                     /locus_tag="VNR65_11655"
     CDS             2376..2750
                     /locus_tag="VNR65_11655"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWJ19369.1"
                     /translation="MADNPFEIPKNMRDLAEQNMKQAHAAYDQLTDFVTKAMGTWMDA
                     MPSNPVAAGFKDIQDRAVSMAKKNADSAFSVAEKMAKAQNLQEIVGLQTRFAQEQMQN
                     YTAQTQELHKLIGDAVQKTTRT"
     gene            complement(2883..>3521)
                     /locus_tag="VNR65_11660"
     CDS             complement(2883..>3521)
                     /locus_tag="VNR65_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19370.1"
                     /translation="PARIIILRHAEKLNRHELCDMGEERSQALSRQFLGRGASQSLFP
                     AGQKPDAFLAITLHTIETITPAAQSWNLPVIPYEVSGRKEDAEEKEAELNQRTQEAAH
                     DVLTDPRYDGKTVVIAWEHNHIAKSKLEKEYAGEQITFRQLLHLDQTANVPKTWPDSN
                     YDYFWIVDYAPGNPVPTGFQMVRQTFAAPFDKLPANEWDQPEPEHMEAGCKK"
BASE COUNT          793 a          987 c         1008 g          733 t
ORIGIN      
        1 ccgtggatga aggcttcgag cttgatgtcc gggcaccgct cgcggatttc cttcagttcc
       61 tcgaacgaca cctcgcgcgc gagaacgatg aggctcgcac caagctcacc ccagaattgc
      121 accgaaagcc atgaacacac attggcctgc gtcgagatat gaagctccaa ctcgggcgcg
      181 gctctcttga cgaaattgaa aacgccgggg tccgcgacga tgacgccatc gggcctcacc
      241 tcgcgaatgg tttgaacgta ttccggcagt ttctcgatat cgcggttgtg cgagaacagg
      301 ttgagggtca gataggcgcg cttcccatgg gcgtgagcga aggcgacgcc ttccaagact
      361 tcgtcgagcg agaagttcga ccgcgtgcgc agcgacagat ccggcgttcc catgtagaca
      421 gcgtcggcgc catacaggat tgcaaccttc agcttctcca gcgaccctgc cggcatcagc
      481 aactcggcgg cgcgttccat gggaaaagcc tcgcgaaatg atttctggtt accctagaaa
      541 tcgccagagt tccagggtag gtcaatggct gcaagcgggt ttctcgacga agaacaatca
      601 tacaatcaag catttcatct gctcgacatg ttcagagacg atattgagtc cgccgtaatg
      661 cgcttcaacg caacatccat cgcttagatc gaggagcgga atcgtgaaaa tcgaaattaa
      721 agccgacaag gacgcagtcc tcggcggtcc tgatcatatt ccatatttaa agctcgaatt
      781 aagagaggac agctatgctg tcttgcacgc gagccatcgg gcgagcggcc ggactgcgag
      841 tcccatggag gagcagagcg aacggatatg cgcatggagc gcgagtcttg atcctggatc
      901 gtatgcgatc ccggaccttg ccgcgattga agcgctcgcg gaccgcctgc atccgttgct
      961 tgcgcgcgta gcggcctggc actccatgga ttgggacggc tcgaactatg ccagcgaagc
     1021 cagcgctgaa gttgaaaaac tcctctgcgc cgcgcaatgg tgggatcgag agcatcaggt
     1081 gtgggacgct gatgattggc ttttcgagct tggatatctt ggcgccgcca aggactatga
     1141 ccttggcgtt gactccaacg aggccgcata caaggctgcg ggcaagaaaa tagaagccga
     1201 tgcccttcgt tatggcgttg tgcttaccaa caccgatgca gtactccgcc gcatcaggca
     1261 agccttgcaa gcagaggcta aagaggacgc ttagcctaag acagcacgag cggagatatg
     1321 ttcattgaca catggcacct ccttcttgta acaggccggg aggcctgcga acgtatcggg
     1381 agatttcatg cggatacttg ctgccggctg gcacggacaa atcgcaagcg cattcatgca
     1441 aatcgcgccc gggcgcaagg acatctcggc ctttgcaatc ggcaggcctg ggctggatat
     1501 ttgcgaaccc cgaagcgtcg aaagggcctt gggagacatc cggcccgacg ttttgatcaa
     1561 tctggctggc tacaccgacg ttgacggagc cgaaagcgag ccggagctgg cccttgcgct
     1621 caattcggcc ggagcgcggc tgctggccga ggctgccgcg cgaagagacg tgcctatcat
     1681 tcatatttca acgagttacg ttttcaacgg gcacaaaaag tctgcctatg tcgagacaga
     1741 ccagacggcc ccttcaacga tctacggaca atcaaagctc gcaggcgaag tcgaggtgca
     1801 aaaggccaac tcgaagcata tcattttgag aacggaatgg ctccacagcc ccttcggccg
     1861 ctgtttcgta agcaatattc tgcagcgtgc gcagctcgga atgccactga aagtcgttaa
     1921 cgatcaatat ggaaacccaa cctacgcccc tcacctggtt gatgcaatcc ttgcagtcgc
     1981 cgcgcatctg acctcaagcc cctccgacgc catcccctgg ggtgtctatc acgctgctgg
     2041 taccgggaca gcgacctggt acgacgttgc tcgtgaggtg cttatcaaat ccgagcagct
     2101 gagggggctt tccttcagcc ttgaggctat aacttccgcc gaatacttga cacgctccca
     2161 tcgctcgccg aatgctcagc tcgattgctc gaagttaaag caaactttcg gcgtcgagtt
     2221 gcctgactgg caagaagggg tccatgagtg cgtcgggcgc ctgctcgccc ctgatttagt
     2281 cgcggtctga atggcttttt gactaatctt gtcacgccgt tgctgcgggc gtgttataac
     2341 ttttcatcaa ttcagaccgc tacgaggagg agaccatggc cgataatcct tttgaaatcc
     2401 cgaagaacat gcgcgatctt gccgaacaga atatgaagca agcgcatgcc gcttatgacc
     2461 aacttacaga tttcgtgacc aaagccatgg gcacatggat ggacgcgatg ccctcaaacc
     2521 ctgtggccgc gggcttcaag gatattcaag accgcgccgt ttccatggcg aagaaaaatg
     2581 ccgattcagc cttctccgtg gccgagaaga tggccaaggc acaaaatctc caggaaattg
     2641 tggggcttca gacccgtttc gcgcaagagc agatgcagaa ctacaccgcg cagacgcagg
     2701 agcttcataa gctgatcggg gatgctgtcc agaagacaac gcgcacctga tcctccgaaa
     2761 cgagtggccg gtgacagacg ccggccctcg tgatgtccgg acgcaggacc acgctgagag
     2821 ccggacgctt ccccggcgga aagggacatt cgatccgcgc gcaggctcaa gtgcttgagg
     2881 gcctatttct tgcagcctgc ttccatgtgt tcaggctccg gctggtccca ctcgttggcc
     2941 ggcaatttgt caaagggggc ggcgaaagtc tggcggacca tttgaaaccc ggtcggaaca
     3001 ggattgcctg gagcgtaatc gacaatccag aagtaatcat aattcgagtc cggccaagtc
     3061 ttcggcacat tggcagtctg gtccaggtgt aagagttggc gaaacgtaat ttgctccccc
     3121 gcatactcct tttcgagctt cgatttggcg atgtgattat gctcccatgc gatgacgacg
     3181 gtcttaccgt cgtagcgggg atcggtgaga acgtcgtgcg ccgcctcttg cgtacgctgg
     3241 ttgagttcgg cctctttctc ctcagcatct tctttcctgc cggacacctc gtaagggatt
     3301 accggcaggt tccagctttg agccgccggt gtaatcgtct caatcgtgtg taaagtgatg
     3361 gctaggaagg catcaggctt ctgtccggcg ggaaacagcg attgcgatgc gccccggccg
     3421 agaaactgac gggagagagc ctgagagcgc tcttcgccca tatcgcaaag ctcatgacga
     3481 ttgagtttct cggcgtgccg cagaatgatg atgcgcgcag g
//
LOCUS       DATMGX010000709         2322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_50287, whole genome shotgun sequence.
ACCESSION   DATMGX010000709 DATMGX010000000
VERSION     DATMGX010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50287"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VNR65_11665"
     CDS             complement(<1..543)
                     /locus_tag="VNR65_11665"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ19371.1"
                     /translation="MSKVAFIGLGVMGYPMAGHIRTKGGHEVTVYNRSLGKAQAWVAE
                     FGGQMAPTPEAAAREADFVFSCVGNDDDLRSVTIAEHGAFHGLKRGAIFIDNTTASAN
                     VARELYAAAAERGAHFLDAPVSGGQAGAQNGVLTVMVGGDAEPFQKAERVISLYARAV
                     KLIGPAGSGQLTKMVNQLCIA"
     gene            complement(648..1415)
                     /locus_tag="VNR65_11670"
     CDS             complement(648..1415)
                     /locus_tag="VNR65_11670"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWJ19372.1"
                     /translation="MFWFIFIASIYLTILVLVSIFRRKAAPAERRVALVIGNAAYKLA
                     PLNNPTKDASDMADALKRLGFEVVECHDLGVAQMRSVFANFETKARAADWALVYYSGH
                     GMEWDGENWLIPIDANTPSQAVSVEHVLRHLRGAKKLRIVILDSCRVNPLHTRMVMNE
                     GLFRVMTQGLARMMPAFGEIVFFAARHNKVALDGKKGENSPFAKALLKHLGEEGLELG
                     RFFRKVTSSVLAATNNFQEPHVYGRIPDEDFYLKPRP"
     gene            complement(1593..>2322)
                     /locus_tag="VNR65_11675"
     CDS             complement(1593..>2322)
                     /locus_tag="VNR65_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWJ19373.1"
                     /translation="ARALQTAATSRRVALVIGNGAYTSVDRLKNPVNDASAVAGALTR
                     LGFDVDERHDLGVVPMQRVLRDFEDKAEGAEWALVYYAGHGLEQNGKNWLIPVDAELT
                     RATDLPDEAIPVERVLDRLSGGRKLRMVILDACRTNPYLARMVMNGATTRSVTQGLAA
                     IEPPHGEVVFYAARDGSVAADGPGANSPFATALLKHMEEEGVELGRFFRKVTSRVLAM
                     TKNQQEPFVYGRIPDEDFYFKPPQ"
BASE COUNT          462 a          708 c          657 g          495 t
ORIGIN      
        1 tgcgatgcag agctggttga ccatcttcgt cagctgcccc gagcctgcag gtccgatgag
       61 tttcacggcg cgcgcataaa gggagatgac gcgctcggct ttctggaagg gttcggcatc
      121 gccgcctacc atgacggtga gcacgccgtt ttgcgcgccg gcctgtccgc cggaaacggg
      181 tgcatccaga aagtgcgctc cacgctcggc cgcggcggca tagagctcgc gggcgacatt
      241 ggccgacgcg gtcgtgttgt cgatgaagat cgcgcctcgc ttcaagccgt gaaacgcgcc
      301 gtgctcggcg atggtgaccg agcgcaaatc atcgtcgttc ccgacgcagg aaaatacgaa
      361 gtcggcttcg cgcgcagccg cttccggcgt tggagccatc tggcccccga actcagcgac
      421 ccaggcctgc gccttgccga gcgagcgatt gtagacggtg acctcatgtc cgcctttcgt
      481 gcggatgtgc ccagccatgg gatagcccat gacgcccagt ccgataaatg caaccttgct
      541 cattggggct ctcctcgttt gctgacctga aatcgattct taatacgcga gagcaacgga
      601 tggacctgca ttttataaag cggcccaggg cttcccggct ctcgcgttca tggccgcggt
      661 ttgagataaa agtcctcgtc cggaatgcgc ccgtagacgt ggggctcctg aaaattgttg
      721 gttgccgcaa gcacgctgct cgtcaccttg cggaagaagc ggcctagctc aagcccctcc
      781 tcccccagat gtttcaagag cgccttcgcg aatggactgt tctcgccctt ctttccatcc
      841 aaggccacct tgttgtggcg cgccgcaaaa aacacgatct cgccgaaggc aggcatcata
      901 cgggctagcc cttgcgtcat aactcgaaaa aggccctcgt tcatcaccat gcgagtgtgc
      961 agcggattaa cgcggcatga atcgaggatc acgatccgca gctttttcgc tccgcgcagg
     1021 tgacgaagga cgtgctctac tgaaacggct tggcttgggg tgttggcatc aattgggatt
     1081 agccaattct ccccgtccca ctccatgcca tgaccggagt aatacaccaa tgcccagtcc
     1141 gctgctctgg ccttagtttc gaaattcgcg aatacgcttc gcatctgcgc taccccgaga
     1201 tcatggcatt caacgacctc gaatccgagg cgttttagcg cgtcagccat gtcagatgca
     1261 tctttcgtgg ggttattgag cggtgccaac ttgtaggcgg cgttgccgat gacgagcgca
     1321 acccgtcgct ccgctggagc ggctttccgc cgaaatatgg aaacaagaac caaaatcgtc
     1381 agatagatgg atgcgataaa aataaaccaa aacatgtcgg ctcattgttt tttttcggca
     1441 aacaatcata gcgctatagt cctttgacct atgctcaagc tacttcttta tgtcgggaaa
     1501 cggcggagca ggatgtttta ctcgggcgcg aagccgcacc gagtaccgtc tgcccttcgt
     1561 cccggcgtta tcggccgctt tttcggtggc gatcactgcg gcggtttgaa atagaagtcc
     1621 tcgtccggaa tgcggccata gacgaagggt tcttgctgat tcttggtcat ggcgagcaca
     1681 cgactcgtca ccttgcggaa aaagcggcct agctccacgc cctcttcttc catatgcttg
     1741 aggagcgccg ttgcgaatgg actattcgcg ccgggtccgt cggccgcaac gctgccgtca
     1801 cgcgccgcgt aaaacacgac ctcaccatgc gggggctcaa tggcggctag cccttgcgtc
     1861 acagaccgag tggtggctcc attcatcacc atgcgtgcca agtacgggtt ggtgcggcat
     1921 gcatcgagga tcaccatccg cagctttctc ccccccgaca gacgatccag cacgcgctcc
     1981 accggaatag cctcgtccgg gaggtcagtg gcgcgggtta gctcggcatc gactgggata
     2041 agccaattct tgccattctg ctccaagcca tgcccggcat aatacaccaa ggcccattcc
     2101 gccccttcgg ccttatcttc gaagtccctg agcacgcgtt gcatgggcac aacgccgagg
     2161 tcgtgtcttt catcgacatc aaagccgagg cgggtcagcg cgccagccac ggcagatgcg
     2221 tcattaacag ggttcttcag ccggtcgacc gaagtatagg ctccgttgcc gatgacaagt
     2281 gccacacggc gtgacgttgc agcggtctgg agcgctcggg cc
//
LOCUS       DATMGX010000710         5103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_164905, whole genome shotgun sequence.
ACCESSION   DATMGX010000710 DATMGX010000000
VERSION     DATMGX010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164905"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VNR65_11680"
     CDS             complement(<1..1187)
                     /locus_tag="VNR65_11680"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HWJ19374.1"
                     /translation="MTSPTTNAKLRAWVDEMTALCRPDHVVWTDGTQKEYDQLCQMMV
                     DTGTFIRLNPEKRPNSYLARSHPSDVGRVEDRTFICSVNKNDAGPTNNWAAPAEMRAT
                     LNGLFDGCMTGRTMYVIPFSMGPIGSPIAKIGVQITDSPYVVTNMRIMTRMGKAVLDA
                     LGPDGSFIPCMHSIGAPLKPGQKDVPWPCEGDINRKYIVHFPETYEIWSYGSGYGGNA
                     LLGKKCLALRIASVMARDEGWLAEHMLIAGIQSPDGEKTYLGAAFPSACGKTNLAMLV
                     PPKGFEGWKVYTVGDDIAWIKKAPDGSLRAINPEAGFFGVAPGTNTQSNPNAMATISE
                     NTIFTNCALTDDGDIWWEGMTDETPAHLIDWKGEDWKPGCGHPAAHRLCDGARRRLAR
                     RAHA"
     gene            1676..3271
                     /locus_tag="VNR65_11685"
     CDS             1676..3271
                     /locus_tag="VNR65_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HWJ19375.1"
                     /translation="MDSVAGSTAARPRSTGMPWLVRGDIDGFFGLAIDNLVQLLVIVA
                     LCRGVLGFSDNLILGTILPGVAVSLVVGNVFYAWQAKELSRRTGRSDVCALPYGINTV
                     SLFAFIFLVMLPALLAAKARGLGNEEATLAAWRMGLVACLGAGLIEVLAAPFAERLRR
                     ATPRAALLSTLGGIALGFISFSFLFRTYATPIVGFTTLGVILLVYFGKVRFRGGLPGG
                     FVAVMLGIALSFAMGLIPTSPPASQTLGLHFPAPVLGDILAALSWHDILTYLAVIVPM
                     GIFNVLGSLQNIESAAAAGDPFPTAASLAMNGVGTIAAALFGSCFPTTLYIGHPGWKA
                     LGARAGYSILNAALFTVLCLTGSLAFVAWLVPIEAGMAIVLWIGIMMAAQSFEATPRH
                     HAPAVVMGLLPGLGAWGVLMAKDGLRAGHATFSPELVARMTETGNSIGGGFALEQGFI
                     FTAMILSAVTVHLIEKRFASAALWSLAAAALSGVGLMHSYVFTPADTIGSFGVPAWQY
                     AAGYTAMAAVFLGARWLVVEVTH"
     gene            3510..3884
                     /locus_tag="VNR65_11690"
     CDS             3510..3884
                     /locus_tag="VNR65_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19376.1"
                     /translation="MHTLNAMPRGVSALSEKQMRAFRLSDWCCDDRCTLEYGGATRGF
                     RAARQAIPDVRIYSVVAMPKSLCCTAVVTGPRFVLRELGKYAIYGAMLSGFALPTLVP
                     KAERANAPARAARVHLNADKLL"
     gene            3970..4464
                     /locus_tag="VNR65_11695"
     CDS             3970..4464
                     /locus_tag="VNR65_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19377.1"
                     /translation="MDPTDEPFAPAPSEVSGPDHAMSPPPKSSGLERLLLVLAVLSAA
                     LVFLPLTAETALLHWNIIGAGYEFISPLRKLGLFSCLACMAASWLLEKEKEEGGHQAI
                     VFGLLAGLLLTAAWGIETLVPFEGWWQALRLTPAVVYGGLAYTFLAERSGRDRGAPPD
                     DEAA"
     gene            4820..>5103
                     /locus_tag="VNR65_11700"
     CDS             4820..>5103
                     /locus_tag="VNR65_11700"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HWJ19378.1"
                     /translation="MIRFYTKRLERDLKRWHEKGWVTGEGYQAIHAELAHGPKLSASA
                     ALAAIGAILFGFAAISFVAANWNEIPRLARVAMLVSALWAAYGSAAYLYKR"
BASE COUNT          889 a         1518 c         1555 g         1141 t
ORIGIN      
        1 gcatgtgctc ggcgagccag ccttcgtcgc gcgccatcac agaggcgatg cgcagcgggg
       61 tggccgcagc cgggtttcca gtcttcaccc ttccagtcga tgagatgcgc tggcgtctca
      121 tcggtcatcc cctcccacca gatatcgcca tcatcagtga gcgcgcagtt tgtgaagatg
      181 gtgttctcgc tgatggtcgc catcgcgttc gggtttgatt gcgtattcgt gcccggtgcg
      241 acgccgaaga atcccgcttc gggattgatg gcccgcaagg aaccgtccgg cgctttcttg
      301 atccaggcga tgtcgtcccc gacagtgtag accttccagc cctcgaagcc cttcggcggc
      361 accagcatgg cgagattggt cttgccgcag gcgctcggga acgcggcgcc gagataggtt
      421 ttctcgccgt ccggtgattg gatgcccgca atcagcatgt gctcggcgag ccagccttcg
      481 tcgcgcgcca tcacagaggc gatgcgcagc gccagacatt tcttgcccag gagcgcattt
      541 ccaccgtagc cagagccata cgaccaaatc tcgtatgttt ccgggaagtg aacgatgtat
      601 ttgcggttga tgtcgccttc gcacggccac ggcacgtctt tctgtccggg cttgagcggc
      661 gcgccgatcg agtgcatgca tggaatgaag gagccgtccg ggcctagcgc atccagcacg
      721 gccttaccca tgcgcgtcat aattcgcatg ttggtgacga cataggggct gtctgtgatc
      781 tgcacgccga tttttgcgat tggcgagccg atcggcccca tgctgaacgg gatgacgtac
      841 atcgtccgcc cggtcatgca accgtcgaac agaccgttaa gcgtcgcccg catctccgcc
      901 ggcgcggccc aattgttggt gggaccggcg tcgtttttgt tcacggagca tatgaacgtc
      961 cgatcctcga cccggccaac gtccgaagga tgcgagcgag cgagataaga gttcggacgt
     1021 ttctccgggt tgaggcggat gaaggttccg gtatccacca tcatctggca aagctgatcg
     1081 tattccttct gagtgccgtc ggtccacacg acatggtcgg gcctgcacag agccgtcatc
     1141 tcatcaaccc aggcgcgcag cttggcattg gttgtagggc tggtcatgac agcatccatt
     1201 gttttcgcct ccacgatttc gggcatgccg cccagatgcg ctccggtcgc ccctccctca
     1261 ttttgccttg aaaaagggcc ggtcagaggt attttgtcct tctcaacttg aactacaggg
     1321 ttgctgtgta ggcaagagta gattcgcact cgcgccaata ggtaccaggt gcagggtggc
     1381 gaacgagtaa gatttcctgc caacgagcaa gactgccaga ctttgcttct ccagtccccg
     1441 tgtcaagcgg ctgcatcctg aatcgagccc gtgaccgctc tcgatcctgg agagtctaat
     1501 ggcctggccc actttatcgt actcccggat gattccattt ctgcgaattc gttgctcccg
     1561 ctgcccgaca ctcccaggtt ggcggaacca acgcttggcg ccaagaaggt agacaagtca
     1621 acttagcccg cgatagatcg ataagcgaaa gacatgccac cgagggggtg aggcgatgga
     1681 cagcgttgct ggttcgacag cggcaaggcc gcggtcgaca ggaatgccct ggctcgtgcg
     1741 cggagatatc gacggcttct tcgggcttgc gatcgacaat ctcgtccaac tcctcgtgat
     1801 cgtcgcgctg tgtcgagggg tgctcggctt ctcggacaat ctgatcctgg gcacgatcct
     1861 accgggtgtt gcagtctcgc tcgtggttgg caacgtgttc tacgcttggc aggcaaagga
     1921 gctttcgcga cgcaccgggc gtagcgacgt ctgcgcgctg ccctatggca tcaatactgt
     1981 atcgctgttc gctttcatct tcctcgtgat gctgccggcg ctcctggcag ccaaggcgcg
     2041 cgggcttgga aatgaggagg ccacgctggc ggcttggcgc atgggccttg tcgcctgttt
     2101 gggcgccggc cttatcgaag tcctggcggc gccattcgcc gagcgcctgc gtcgcgccac
     2161 tccacgagcc gcgctgttgt ctacgctcgg cggcatcgca ctcggtttca tatcgttctc
     2221 gttcctgttc cggacctatg cgaccccgat tgttggcttc acgacgctcg gcgtcatcct
     2281 gctggtctat ttcggcaagg ttcgtttcag gggcggcttg ccgggcggtt tcgtcgccgt
     2341 gatgctgggt atcgcgctgt cgtttgcgat ggggcttatt ccgaccagcc cgccggcttc
     2401 acagacgctg ggcctgcatt ttcccgcgcc ggtgctaggt gacatcctcg ccgcgctcag
     2461 ctggcacgat atcctgacct acctcgccgt gatcgtcccg atgggcatct tcaacgtgct
     2521 gggctccctg caaaacatcg agtccgccgc tgctgccggc gatccgttcc cgacggcggc
     2581 atcactggct atgaacggcg ttggcaccat tgccgcggca ctcttcggat cgtgctttcc
     2641 caccacgctc tacatcggtc atccggggtg gaaggcgctt ggcgcgcgcg ccggctattc
     2701 catcttgaac gcggcgctgt tcaccgtgct gtgcctgaca ggctcgcttg cattcgtcgc
     2761 gtggctggtc ccgatcgagg ctggaatggc gattgtactg tggatcggca tcatgatggc
     2821 cgcccaatcc tttgaagcga caccgcgtca tcacgccccg gccgtcgtca tgggcctgct
     2881 gccgggtctc ggcgcatggg gtgtgctgat ggccaaggat ggccttcgcg cgggccacgc
     2941 aacattctcg ccagagcttg tggcgagaat gactgagacc ggcaactcca tcggcggtgg
     3001 ctttgccctt gagcagggct ttatcttcac ggcgatgatc ctgtcggccg tcacggtcca
     3061 tctgattgag aagcgcttcg caagcgccgc tctgtggagc cttgccgccg cagcgctgtc
     3121 cggagtgggg ttgatgcaca gttatgtctt cacgcccgcc gacacgatcg gcagtttcgg
     3181 cgttcccgcc tggcagtatg cggccgggta cacggcaatg gcagcggttt tcctgggagc
     3241 ccgatggctg gtcgtcgaag tcacgcacta acgcgaggtc cgagttatac caaccgtcgg
     3301 agcgttcggc tcatctcatc aattggagca tttccaggcg catcatccgg taaggtgtat
     3361 agggggttaa gattggtttt gtttcgtaaa acagctataa gtaaatatat aaatactttt
     3421 tttgccacca agaacggtga tgggcgaagt cgtcgggcct tatggcggtc cgtatgatac
     3481 gagccaaccg gccgacgaga agcgcgaacg tgcacacgtt gaatgcgatg ccacgcggtg
     3541 tgtctgcgct gagtgaaaag cagatgaggg catttcgcct cagtgattgg tgctgcgatg
     3601 accgctgcac cctcgaatac ggaggtgcga caagagggtt ccgcgcggct cgccaagcga
     3661 ttcctgatgt ccgtatctat agcgtggtcg caatgcctaa aagcttatgt tgcactgctg
     3721 tcgtgacggg gcctcgcttc gtgcttcgcg agcttggcaa atatgcaatc tatggcgcga
     3781 tgctttcggg ttttgcgctg ccgacgctcg tgcccaaggc cgagcgcgcc aatgcgcccg
     3841 ctagagcagc ccgcgtccat ttgaacgcgg ataagctgct ctaacgactt agaatcgatc
     3901 aacttttccg cctttaatcg gctccgatta aaggcgggtt gatctagcgt tgctttgcgg
     3961 tgaagccgaa tggacccaac agacgagcct tttgcgccgg cgccaagcga ggtaagcggg
     4021 ccggaccacg caatgagtcc tccgccgaaa agctccgggc tcgaaaggct gctgctcgtg
     4081 ctcgcggttc tttcggcggc gctggtcttt ttgccgctga cggccgagac ggccttgctg
     4141 cattggaaca tcattggcgc aggatatgag ttcatctcgc cgctccgcaa gcttgggctc
     4201 tttagctgcc tggcttgcat ggcggcatct tggcttctgg aaaaggagaa agaagagggc
     4261 gggcatcagg ccatcgtttt cggcctgctc gccggccttc ttctcaccgc tgcttggggc
     4321 atcgagacgc ttgtgccctt cgagggctgg tggcaggcct tgcggctcac tcccgcggtg
     4381 gtttacggcg gcctcgccta tacgttcctg gcagagcgtt ccggcaggga tcgcggcgcg
     4441 cctccggacg atgaggcggc atgatgggcg cgcggccgcc agcgcttggg gcatttggaa
     4501 agcgctctcc ctgaccaccc tgctcactgc tagcgatcga ggcacaggcg gctaaggtat
     4561 aaagcctgat gtcagaccgc ttttccgtca cacggcatcc acgatacggc cacggttcgc
     4621 ggaacaatgc tccgcacgcg ccctaagcgg acagttcttg gtcagccgac gaatgtcttc
     4681 ttagagcctt ttgtttacgc agagtttttt tccaaaaccg ggaaggccac ctttgggaac
     4741 tctgcttaga tcaacccgct attaatcgga atcgattgaa ggtggaaaag ttgatcgcta
     4801 gagcaaggag tgtaaaacct tgatccgttt ctataccaag aggctcgagc gcgaccttaa
     4861 gcgctggcac gaaaagggct gggtgaccgg ggagggatac caggcgatcc acgccgagtt
     4921 ggcgcatggc cccaagctat cggctagcgc tgcgctcgcg gccataggcg cgatcctgtt
     4981 cggcttcgcc gcgatcagct tcgtcgctgc caactggaac gagattccaa ggcttgcccg
     5041 cgtggcgatg ctcgtatccg cactgtgggc cgcctatggc agcgccgctt atttgtacaa
     5101 gcg
//
LOCUS       DATMGX010000711         2102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_11680, whole genome shotgun sequence.
ACCESSION   DATMGX010000711 DATMGX010000000
VERSION     DATMGX010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11680"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VNR65_11705"
     CDS             complement(<1..1175)
                     /locus_tag="VNR65_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWJ19379.1"
                     /translation="MAGLFLLGGCTLLNPHVPTPEVEVPDAYIGKSANPSKPAASPYE
                     FAVFKSKKLTELIQLGRGFNFDIGAAIARIQEAEAQVRIATQALIPLVQANGSASQSL
                     THVGGQAVKSTSVIAQLNASYTIDFWGQNRALVYAAIANQYNQSFAAATVAISTDASI
                     ANTYFEAIGTQEQIDIAKRNLEAAKRILKAIRDRFAAGTASGLDVAQQETLVDNIAVT
                     IPPLERQLEQFKHALAVLVGTAPEFFSYKGDKLFSIAVPTIPAGLPSELLCRRPDIAS
                     AEAQLSTAKFDVSSARAAMFPSIQLTGNGGFQSVALASLFQPQSVFYNAAVGVTQPIT
                     NEYQLQAQLDLQRATYGEFLQDYRKSIVSAFQNVEDALIAYSKDAEQERLQREAVMSA
                     "
     gene            complement(1459..>2102)
                     /locus_tag="VNR65_11710"
     CDS             complement(1459..>2102)
                     /locus_tag="VNR65_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HWJ19380.1"
                     /translation="PAMTRLQARLTLFTVMAIFAATASNALFLQDRARLLNGSGLPST
                     AVSLTQLSADKAAPDNAGASALLEEDPKAPRLHIALQRELARRGYANQLRARPNGLRL
                     AVLAYEFDNSLPLTGEPTEALLKRVLFDGNQGPRGSFTDRAEINAKLVMEAQRMLAGL
                     GFFRGTFSGRIDVWTSNAIKDFERHRGIPQTGRLTDQTLLELISYSGQPIRLS"
BASE COUNT          420 a          617 c          628 g          437 t
ORIGIN      
        1 gcggacatca ccgcttcgcg ctgcaggcgc tcctgctcag cgtccttgct gtaggcaatc
       61 aaggcatctt cgacattctg gaacgcggaa acgatactct tgcggtaatc ctgaagaaat
      121 tcaccgtagg ttgcacgctg gagatcgagt tgggcctgca gctgatactc gtttgtaatg
      181 ggctgggtga cgccgaccgc cgcattgtag aacacgcttt gcggctggaa cagggacgcc
      241 agcgccacgc tctggaagcc gccattgcct gtgagctgga ttgagggaaa catcgccgcc
      301 cgcgccgaag agacatcaaa ctttgccgtt gagagctggg cctccgcgct tgcgatatcg
      361 gggcggcggc acaaaagctc ggacggcaag ccggccggga tagtaggcac cgcgatcgaa
      421 aaaagtttgt cgcctttgta actgaaaaac tcaggcgccg tgcccacaag gacggccagc
      481 gcatgcttga attgctcaag ctgccgctcg agcggcggga tcgtgaccgc aatgttgtcg
      541 acaagcgtct cctgctgcgc cacatcgagc ccgctcgccg ttcccgccgc aaagcggtcg
      601 cggatggctt tcaggatgcg tttcgccgct tccagatttc gcttcgcaat atcgatctgc
      661 tcttgggtac cgatcgcttc gaaataggta ttcgcgatac tcgcgtctgt cgaaatggcg
      721 acggtggctg cagcgaagct ttgattgtac tggttcgcta tcgcagcgta cacgagcgcg
      781 cggttctgac cccagaaatc gatcgtgtaa cttgcgttca actgcgcgat gaccgatgtt
      841 gattttacgg cttggccgcc gacatgggtc aggctctggc tcgcgctacc gttggcctga
      901 acgagcggaa tgagcgcctg cgtcgcgatc cgaacctgcg cttcggcctc ctgaatgcgg
      961 gcgatcgccg cgccgatgtc gaaattgaag cctcgcccta gttgtatcaa ctcggtcagc
     1021 ttcttcgact taaacaccgc gaactcgtag ggcgacgccg ccggcttcga tgggttcgcg
     1081 ctcttaccga tataggcgtc gggaacctcg acctctggcg tgggcacatg cggattgagg
     1141 agtgtacacc cgccaagcaa aaaaagcccg gccactcccg ctattcgggc tccgctccgg
     1201 tttaccgccc tacccgattt atgtcgcgcg ccccagcgtt gcatgcccgc tcgatttgaa
     1261 tgagtttcgg tccggctctt cccaccctca aacaaagaga atcgctgcaa ttaagaacgc
     1321 tttgaattat gactgttaag gggttgttta cctcggacgc caccgcaaaa agtgtaacga
     1381 tttgcgattg ccgcgttcag gcgcggaggc gtgccttttt ggcaccaagt ttatgcttgg
     1441 gaaggcttta gcctcggctt atgacagccg gatgggctga cccgagtagg aaatcagctc
     1501 aagcagggtc tgatcggtca agcggcccgt ctgcgggatg ccgcgatgcc gctcgaagtc
     1561 tttgatcgcg ttggaggtcc agacatcgat gcggccggag aacgttccgc ggaagaagcc
     1621 aagccccgcg agcattcgtt gggcttccat cacaagcttc gcgttgatct ctgcgcggtc
     1681 ggtaaaagag ccgcgagggc cttgattgcc atcaaaaagg acgcgcttca gcaaggcttc
     1741 ggtgggttcc ccggtcaaag gcaagctgtt atcgaattca taggcgagaa cggcaagccg
     1801 aaggccattt ggacgcgccc gaagctggtt cgcatagccg cgccgcgcaa gctcccgctg
     1861 gagcgctata tgcaagcgag gcgctttcgg atcttcttcc aacaaagcgg atgcgccagc
     1921 attgtccggc gctgccttgt cggccgacag ctgcgtcagt gagaccgcgg tgctgggaag
     1981 gccactccca ttcagcaaac gcgcccgatc ctgcaggaac agcgcattac tcgccgtcgc
     2041 cgcgaagatc gccattactg tgaacaaggt caggcgcgcc tgtagccggg tcatggccgg
     2101 aa
//
LOCUS       DATMGX010000712         4418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_5233,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000712 DATMGX010000000
VERSION     DATMGX010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5233"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /gene="tig"
                     /locus_tag="VNR65_11715"
     CDS             <1..1070
                     /gene="tig"
                     /locus_tag="VNR65_11715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWJ19381.1"
                     /translation="LEKEAAEPDEDDVKKALERLMKANTSYEAKDGAAEEGDRLTIDY
                     KGSVDGEPFEGGTGEDAFVIIGSKRFIPGFEEGLEGAKSGEQREVRVTFPEDYQAKHL
                     AGKDAVFDVTVKEVAKPVETKADDELAKSLGMESFAKLEEAIRGQLQKELDTISRNKL
                     KKALLDALDEKHSFEMPPSLVDSEFEAVWRNITRQLEQSKGSFEAQGTTEEKAREEYR
                     KLAERRVRLGLILAEIGNVNKIQVGDDELRRAVIDRARQFPGQEKQVVEFYRKNPQAM
                     NELRAPVYEDKVINFALELVKADEKKVTPAQLMEFAAEQEREQALLEGGDDGHEHHHH
                     EGECGPECDHDHDHGHHHHHE"
     gene            1250..1891
                     /locus_tag="VNR65_11720"
     CDS             1250..1891
                     /locus_tag="VNR65_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWJ19382.1"
                     /translation="MRDPVEYYTSQLVPMVVEQTNRGERAFDIYSRLLKERIIFVTGV
                     VEDQMASLVTAQLLFLESENPKKEIAMYINSPGGHVTSGLGMYDTMQFIRPPVSTLCV
                     GQAASMGSLLLAAGEPGLRYSLPNARVMVHQPSAGFQGQASDIERHAEDILKTKRRLN
                     EIYVKHTGQPYEIIERTLDRDHFMTAEQAKAFGIIDEVITKRAPVEEAGSSRS"
     gene            2175..3437
                     /gene="clpX"
                     /locus_tag="VNR65_11725"
     CDS             2175..3437
                     /gene="clpX"
                     /locus_tag="VNR65_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWJ19383.1"
                     /translation="MTKESRGHDSKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVEL
                     CMDIIREENKNSYVKSRDGVPTPQEICNVLDDYVIGQEYAKRVLSVAVHNHYKRINHS
                     AKNNDVELAKSNILLMGPTGCGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVEN
                     IILKLLQAADYNVDRAQRGIVYIDEVDKISRKSDNPSITRDVSGEGVQQALLKIMEGT
                     VASVPPQGGRKHPQQEFLQVDTTNILFICGGAFSGLEKIIAQRGRTTSIGFGAKVISD
                     DERRTGEILRSVEPEDLLKFGLIPEFIGRLPVLATLEDLDEVALVKILTEPKNALVKQ
                     YQRLFEMEGVQLTLSTEALKSVSRKAIERKTGARGLRSIMESILLDSMFELPSLKGVE
                     EVVISPEVIEGNAKPLRIYAERLDESSA"
     gene            3676..>4418
                     /locus_tag="VNR65_11730"
     CDS             3676..>4418
                     /locus_tag="VNR65_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWJ19384.1"
                     /translation="MTNAHSDQSKTAGPDEVFPVLPLRDIVVFPYMIVPLFVGREKSI
                     NALDEVMRTDRQILLAAQKNAGDDDPEPDAIYTMGTLATVLQLLKLPDGTVKVLVEGT
                     TRAKIKRFSPNELYFEAEVDRVEETLGNQDEIEAFSRSAVSQFENYVKLNKKISPEVL
                     STLSQIDDYSKLADTIASHLAIKIGDKQEILELLSVSDRLERVFTLMESEISVLQVER
                     KIRSRVKRQMEKTQREYNLNEQMKAIQKEL"
BASE COUNT         1032 a         1224 c         1331 g          831 t
ORIGIN      
        1 cgctggagaa agaggccgcg gagccggacg aggacgacgt caagaaagcg ctcgagcgtc
       61 tcatgaaggc gaatacctcc tacgaggcga aggacggcgc cgctgaagag ggcgaccggc
      121 tgaccatcga ttacaaggga agcgtcgatg gcgagccttt cgagggtggc acgggcgagg
      181 atgcctttgt aatcatcggc tcgaagcgct tcattccggg cttcgaagag ggccttgaag
      241 gcgcgaagtc aggcgagcaa cgcgaagtcc gcgtgacgtt ccctgaggat tatcaagcaa
      301 agcaccttgc cggaaaagac gctgttttcg acgtgaccgt gaaggaagtc gccaagccgg
      361 tcgagacaaa ggctgacgac gaattggcga agtccctcgg catggagtcg ttcgcgaaac
      421 tcgaagaggc tatccgcggc cagctccaga aagagctcga tacgatctcc cggaacaagc
      481 tgaaaaaggc tctcctggat gcgctcgatg agaagcattc cttcgaaatg ccgccctcgc
      541 ttgtggacag tgagttcgag gcggtgtggc gcaatatcac gcgtcaactt gaacagtcca
      601 agggaagctt cgaagcgcag ggcacgaccg aggagaaggc ccgcgaggag tatcggaagc
      661 tcgccgagcg tcgcgtgcgg ctgggcctca tcctcgccga gatcggcaat gtgaacaaga
      721 tccaagtcgg agatgacgag ctgcgccgcg cggtcataga ccgggcgcgc caattcccgg
      781 gacaggagaa gcaggtcgtc gaattctatc gcaagaaccc gcaggcgatg aacgagctgc
      841 gcgctccggt gtacgaggat aaggtcatca acttcgcgct tgaactggtg aaagcggacg
      901 agaagaaggt tacaccggcg cagcttatgg agttcgctgc ggagcaggag cgcgagcaag
      961 cccttctgga agggggcgat gacggccacg agcaccacca tcatgaaggg gagtgcgggc
     1021 ctgaatgcga tcacgaccac gatcacggcc atcaccacca tcacgagtag gcaatgcgcc
     1081 gggccgccat catggctagt tgcgcccgcg ctcaaatcgt cccatttatg cggtgcgggc
     1141 cgggggtccg caccggtctt tttcagccgc cgagttctcc gcccgcgaga tcgcggagca
     1201 accatagaaa agccaatccg ccgcggcaga ttatgccagg gagaaacgca tgcgcgatcc
     1261 agttgaatac tacaccagcc agctcgttcc catggttgtc gaacagacaa accgcggtga
     1321 gcgtgccttc gatatctatt cccggcttct gaaggagcgc attattttcg taacgggcgt
     1381 cgtggaggac cagatggcct ctctcgtcac ggcgcagctt ctcttccttg aatcggaaaa
     1441 cccgaaaaag gaaattgcca tgtacatcaa ttcgccggga ggccatgtga cctccgggct
     1501 cgggatgtac gacaccatgc agttcatacg cccgcccgtt tcaacacttt gtgtcggtca
     1561 ggccgcatcg atgggctcgc ttttgctcgc ggctggagaa cccggcctgc gctattccct
     1621 cccgaacgcg cgggtgatgg tgcaccagcc ttccgcggga ttccagggac aggcgagcga
     1681 tatcgaacgg cacgcagagg acatcctcaa gaccaagcgc cgcctcaacg agatttacgt
     1741 gaagcacacg ggccagccat acgaaatcat tgaacgcacg ctggatcgtg accacttcat
     1801 gacagcggag caggcaaaag ctttcggcat tatcgacgag gtcataacca agcgcgcgcc
     1861 ggtcgaagag gcggggtcca gccgctcgta ggctgaacct gtgtcttcct tgcgacggca
     1921 aaaacagatg cgcgattttt aacggcctgg aaagtatagg cggttcaacc tagcgcttgc
     1981 ctctccgggc ttcgcgaatc gaaattatgg acaatcgacg cagaagccgt aagactatgg
     2041 ggtagcgtac aagggagagt gggacagcga gacaggcttg gggtggaagt tacgccggga
     2101 gaacggggtt gcagtcatca gtcgggcaca atcgaaggcc agctttcctg gtatgtgggt
     2161 gctaaggtag gtcaatgacg aaagaaagca ggggtcacga ctccaagaat acgctctact
     2221 gctccttttg cggaaagagc cagcacgaag tgcgtaagct cattgcaggg ccgaccgtgt
     2281 tcatctgcga tgaatgcgtt gagctttgca tggacatcat tcgcgaggag aataaaaatt
     2341 cctatgtaaa gtcccgcgac ggcgtaccga ccccccagga aatctgcaac gttctcgacg
     2401 attatgtgat cggccaggaa tacgcgaagc gcgtcctctc ggttgcggtc cataatcact
     2461 acaagcgcat caaccactcc gcgaagaaca acgatgtgga gttggcaaaa tcaaacatcc
     2521 tccttatggg ccccaccggc tgcggcaaga cgctgctggc gcaaaccttg gcacgcattc
     2581 ttgacgttcc gtttacaatg gcggacgcca cgacgctgac cgaggcgggc tatgtgggtg
     2641 aggatgtgga aaacatcatc ctcaagctcc tgcaggctgc ggattacaat gtcgaccggg
     2701 ctcagcgcgg catcgtctat atcgacgagg tcgacaagat cagccggaag tccgacaacc
     2761 catcgatcac gcgggacgtc tcgggcgagg gcgttcagca ggccttgctg aagatcatgg
     2821 aaggcacggt tgccagcgtt ccacctcagg gtggccgcaa gcacccgcag caggagttcc
     2881 tccaggtcga cacgaccaac atcctgttca tctgcggcgg cgccttctca ggcctggaaa
     2941 agatcatagc tcagcgcggg cgcacaacct ccatcgggtt cggcgcgaaa gtcatatctg
     3001 acgacgagcg ccgcacaggg gagatcctgc gcagcgtgga gccggaagac cttctgaaat
     3061 tcggcctcat accggaattc attggccgtc tgccggttct tgcgaccctg gaagacctcg
     3121 acgaggtggc tctggtcaag atcctcaccg agccgaagaa tgcccttgtg aagcagtatc
     3181 agcgcctttt cgagatggaa ggcgtgcaat tgacgctgtc gaccgaggca ctgaagtcgg
     3241 tctctcgcaa agccatcgag cgcaagactg gggcacgcgg tttgcgttcc atcatggaat
     3301 ccattcttct cgacagcatg tttgagctgc caagcttgaa aggcgtcgag gaagtcgtca
     3361 tcagtcccga ggtaatcgaa gggaatgcca agcccttgcg gatttacgcg gaacgcctag
     3421 atgaatcgag tgcataatgc aggggtttgt taacaacgaa ccgaagcgtt atgttccacg
     3481 atcccgccaa catttttcga agcacccttc cggtgcaagc gacgcgctgc aattaaccgg
     3541 ctttgcgcat tcctatgggg gtgcgcggcc tgccggtgta cccttgcccg ggcttggtgc
     3601 atcgggcaag tatccgggtt aagccggtga aagtaagcgg tggcacgatt tcgctgccgg
     3661 atttaggaga ctgacatgac gaacgcacat tcggatcaat ccaaaacggc tggtcctgac
     3721 gaagtttttc ccgtcttgcc gctccgtgat atcgttgttt ttccgtatat gatcgtgcct
     3781 ctttttgtcg gacgcgagaa atccatcaac gctcttgatg aggtgatgcg caccgatcgc
     3841 cagatccttt tggctgctca gaaaaacgcc ggagacgatg atcccgagcc agacgccatt
     3901 tacacaatgg gcacgctggc aacagtgttg cagcttttga agcttccgga cggcactgtc
     3961 aaggttctgg ttgagggcac cacgcgcgcc aagatcaagc gcttcagccc gaacgagctt
     4021 tatttcgagg ccgaggtgga ccgcgtcgaa gagacgctgg gcaaccagga cgagatcgag
     4081 gccttctccc ggtcggcggt gagccagttc gagaactacg tcaagctcaa caagaaaatc
     4141 tcgcccgagg tgttgagcac gctcagccag attgacgatt attcgaagct cgccgacacc
     4201 atcgcatcgc atctcgccat caagatcggc gacaagcagg agatcctgga gcttctttcg
     4261 gtctcggatc ggctggagcg cgtgttcacg ctcatggaga gcgagatctc tgtgctgcag
     4321 gttgagcgca agatccgcag ccgcgtgaag cgccagatgg agaagacgca gcgcgagtac
     4381 aacctgaatg agcaaatgaa ggccattcag aaggaact
//
LOCUS       DATMGX010000713         3072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_172011, whole genome shotgun sequence.
ACCESSION   DATMGX010000713 DATMGX010000000
VERSION     DATMGX010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172011"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VNR65_11735"
     CDS             <1..650
                     /locus_tag="VNR65_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWJ19385.1"
                     /translation="RGAVLKAARDALADGEPRMVSVQPENLLAELGVKPGENRDGVRF
                     ASNMCPSKGTMDVFVEPVLPHPSLVILGGSPVALSLAAQARQLGYHVTVAAPAADRTD
                     TPDADMLIDGYALGHLNQAKRFIVVSTQGKGDEAALRTAVKTEAAYHAFVGSRRKMAA
                     LREKLITEGIDATALDRVKAPAGLDLGAITPEEIAMSILAEITVERRRGQRSAAP"
     gene            complement(847..1785)
                     /locus_tag="VNR65_11740"
     CDS             complement(847..1785)
                     /locus_tag="VNR65_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19386.1"
                     /translation="MAPVTCDYGALGQHAWWLLLGFLIVGVFCGAALLKRDWAGFKIY
                     SFAGVGCFIAIFINPLGWHIYDGVAATLGHFVQAYITEWLSFYQNISMPGSMPGILYI
                     SIFVGLELRYRDSCPVESRLLAWLFLFLGLYQFRYLSFFFLFSAVPLAFHLDRLLPKQ
                     LNNLEVQKSLLAAGIIGACALPLTFMQVEPALGLPEMISEQDARYLQTHFPHARLLNH
                     WNVGGTLIFFTRGAVPVFVDGRAATAYPDDLLRDYFKLLELEIDETAWDTVLEKYQID
                     TVLWVKAHEELRRFLVGKRGWKEEYAGLYESIYVKP"
     gene            2661..2945
                     /locus_tag="VNR65_11745"
     CDS             2661..2945
                     /locus_tag="VNR65_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19387.1"
                     /translation="MGIGHPPRKKQDRLGVDNVVFLPRDEFFDVLSPLLEEAATAKDE
                     DSPRQELHKVLSLSKENARLRALAVGLSNLLGDLPVRAWEDAARSCRTKA"
BASE COUNT          755 a          877 c          853 g          587 t
ORIGIN      
        1 cccgcggcgc ggtgctcaag gccgcgcgcg acgcgctggc cgacggcgaa ccgcgcatgg
       61 tctcggtaca gccggaaaat cttctggccg aactcggcgt caagcccggc gagaaccgcg
      121 acggcgtgcg tttcgccagc aacatgtgcc cgagcaaggg caccatggac gtgttcgtcg
      181 aaccggtgct gccgcatccc tcgctggtca ttctcggcgg aagccccgtg gcgctgtcgc
      241 tggccgcgca ggcgcgccag ctcggctacc acgtcacggt cgccgcaccc gccgcagatc
      301 ggaccgacac gcccgacgcc gacatgctga tcgacggcta tgcgctcgga catctcaacc
      361 aggcaaaacg cttcatcgtg gtctcgacgc agggcaaggg cgatgaagcc gcattgcgga
      421 cggccgtgaa gaccgaagcc gcctaccacg ccttcgtcgg cagccgccgc aagatggcgg
      481 cactgcgcga aaaattgatc actgagggca tcgacgccac ggccctcgac cgcgtcaagg
      541 ctcccgccgg cctcgatctc ggcgccatca cgccggagga aatagcgatg tcgattttgg
      601 cggagatcac ggtggaacgc aggcggggac agcggagcgc ggctccttag cagccgtcat
      661 cgcgacgagc gaagtgtccg tcatgctcga tgagcaagga cggaatcggc gaagcgcgct
      721 ccatactttc ttcgtggcgc tttaccggga ttgcttcgcc cggttggcgg aacggacttg
      781 gcgtacgccg cccgaactct gaaattgagc aaaagcggat agccgcgcaa aaaacaccat
      841 gcggcgttat ggcttaacgt aaatgctctc atacagtcct gcgtattctt ccttccagcc
      901 tctcttgccc acgagaaacc gcctcaattc ttcatgcgcc ttcacccaaa gcaccgtatc
      961 gatttggtat ttctcaagca ccgtgtccca agcggtctcg tcaatctcca gttcgagtag
     1021 cttgaaatag tcacgcagca aatcatcggg ataggcggta gctgcccggc cgtccacgaa
     1081 cacaggtacg gcgcctcgcg tgaagaagat caaggttcca ccgacgttcc agtggttcag
     1141 aagccgcgca tgcggaaaat gtgtttgaag ataacgagca tcctgctctg aaatcatttc
     1201 cggaagtccg agtgccggtt ctacttgcat aaaggtcaat ggcagcgcgc acgctccaat
     1261 gatgccagca gccaacaacg acttttggac ttcaagatta ttcagctgtt tgggcagcag
     1321 ccgatcaagg tgaaaagcca gcggaaccgc cgagaataag aaaaagaacg acaagtatct
     1381 gaattgataa agtcctagaa acaaaaagag ccacgcaagc aaccgggact ctacaggaca
     1441 agaatccctg taccgcagtt caagcccgac aaatatcgaa atatacagga tgccaggcat
     1501 gctccccggc atggatatgt tctgataaaa agacagccac tcggtgatgt aggcttgcac
     1561 gaagtggccc agtgtagccg ctacgccgtc atagatatgc cagccaagcg ggttgatgaa
     1621 tattgcaata aagcaaccga ctcccgcgaa actgtagatt ttgaaacctg cccaatcccg
     1681 tttaagcaga gccgcgccgc aaaaaacccc aacaatcagg aagccaagca ggagccacca
     1741 tgcatgttga cccaaagcac cataatcgca ggtaacagga gccatctcgt tctcttcaaa
     1801 cattcaccgt agaatattac ggaaaacaac atggtcgaag tgtttggtga agcggcgaga
     1861 tagacattcg ggggcgttgc aaaagatggg tagagcaaac acgcggaaaa aaccgaaatg
     1921 ctgactgcag ccgccgaggc tccgctgctt aagcagacgg acgcaagata tccgacaatg
     1981 actgcgccac acgcaactac aaataaggtg aggccgccaa atttcatata ttgaaatagt
     2041 acgctggcaa tcacgtccca aagccaggaa agattatacc attgcctgcc tcccgacgtg
     2101 aatgaccatg gatccccgaa aggaatctta ccttggtctc tgatcaagtc tccagcggcc
     2161 aggtgccatc ccaagtcata atggccaagc atgagcgacg cgttcgaaag gtaatacacg
     2221 catgcgaacg aaatcaggaa aatataaatt ccggaccttt gaaagtaaga actgatcgtg
     2281 tttggccctc gatcaaactt ggagtcggcc caagcctcct gcgcgtccat aaatgagccc
     2341 ctcacgatcg aagcgagcct gctgcgtgct tttagggtct gcatggcttg atgcaaacaa
     2401 ccgtcgcttt aggacgatcg gaataatgtt aagtaaaatt cggttgtgta attcgatttt
     2461 ctcgcgcttg ggaatattcc tgcttaacca atgcttacga gggaaaacgc gggcgtacgg
     2521 gtaacgagag ccggtcatcg ccatcggtat gccggcgtgg ctcagttggt cctgttttgg
     2581 actcaaattc gcccccgact cacgtcaggc tgacgggatt cgattggatt aggagtacct
     2641 cctaaatcga gggacttgcc atgggcatcg gacacccgcc gcggaaaaag caggatcgcc
     2701 tcggtgtcga caacgttgtg tttctgccgc gtgacgaatt tttcgacgtt ttgtcgccgc
     2761 tgctcgaaga ggctgcgact gccaaagacg aagattcgcc acgtcaggaa ctccacaagg
     2821 ttctgtcgct ctcgaaggag aatgcccggc ttcgcgcgct tgcggttggg ctatcgaacc
     2881 tcctcggaga cctccctgtt cgcgcgtggg aggatgccgc tcgatcttgc cgcacgaagg
     2941 cgtaagcgcc atgcgctcag gggaaattgg gtgatcctct atcaggcgca cgtagttggt
     3001 tgggaccctt ggggtgggca aagccaacgg gtcgcgcgaa cgcgcgcccg atgatgccag
     3061 gcacggcgca ag
//
LOCUS       DATMGX010000714         2842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_195998, whole genome shotgun sequence.
ACCESSION   DATMGX010000714 DATMGX010000000
VERSION     DATMGX010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195998"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VNR65_11750"
     CDS             <1..515
                     /locus_tag="VNR65_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWJ19388.1"
                     /translation="IVYYPLSANSHADGVLHHSVAPYPNPALLRSARIYLKRVMSALD
                     YVGVLTIEFFVVKGRLIANEMAPRVHNSGHWTIEGCVTSQFENHLRAICDLPLGSTRT
                     LGHAAMINFLGTMPDRKRLLQIDGLAFHDYGKEARPGRKLGHCTILKAGIADRNRALL
                     HALNLIKPAD"
     gene            523..1755
                     /locus_tag="VNR65_11755"
     CDS             523..1755
                     /locus_tag="VNR65_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ19389.1"
                     /translation="MYLELFKLRELPFRLSPDPQFLYLSKQHARAKAYMESTIWFTDG
                     FVVITGEIGSGKTTLIETILRELQGNVVVAQINQTQLSPTEFLQTILVQFGFTPFDMK
                     KPEVLATLNQFLVEQHVNGRKVLLIIDEAQNLSNRVLEELRMLSGIETTKDKVLRIIL
                     AGQPELNDKLDSRELIQLAQRVRLRFHLSTLSQAETSAYIDHRLEVAGSQGRRIFEED
                     TYPVIYKYTGGVPRLINTLCDTAMMAAFSRDRDTVALHDLEAAIEELQWVEFNANTDR
                     MRVLRMEHAPPPHVKNGPVVGRILLASGGKTVMERELRPGRLVIGRTADNDIQIDSKF
                     ISRHHCQVVTLRDSCLIEDLNSTNGIYVQSKRVRRYNLNDGDVVHLGQHEIMYIDERA
                     PRLRDGTDPSGANDAEGH"
     gene            complement(1770..2006)
                     /locus_tag="VNR65_11760"
     CDS             complement(1770..2006)
                     /locus_tag="VNR65_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19390.1"
                     /translation="MRLAKPVYEILPVIYVVIGALAILLVCADPRGPYALMVLGIGLL
                     AEIAALTVFLHRQDCRAHSREYPGESIDLPTLNR"
     gene            complement(2112..2276)
                     /locus_tag="VNR65_11765"
     CDS             complement(2112..2276)
                     /locus_tag="VNR65_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19391.1"
                     /translation="MSDKSMTGTFQLNLPKELLSESARQSKRSKEQLQIEGWRNSWLS
                     RLSELLVGRE"
     gene            complement(2461..>2842)
                     /locus_tag="VNR65_11770"
     CDS             complement(2461..>2842)
                     /locus_tag="VNR65_11770"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWJ19392.1"
                     /translation="HAGGCLCGGVRYRIQGALRGVIACHCMQCRKTSGHHAAMTSVPA
                     ANLSLVSSHTLSWYKSSDTAERGFCRVCGGNLFWSEFGKDTISVTAGTLDAPTRLALS
                     EHLFVADKSDYYQINDDLPKKAQW"
BASE COUNT          626 a          870 c          817 g          529 t
ORIGIN      
        1 acatcgtcta ctacccgttg tccgccaaca gccatgcgga cggcgtcctg caccatagcg
       61 tggcgccata cccaaacccc gccctgctgc gcagcgccag gatctatttg aagcgcgtga
      121 tgagcgcgct cgactacgtc ggcgtgctca ccatcgagtt cttcgtcgtg aagggccggt
      181 tgatcgccaa cgagatggcg ccgcgcgtcc acaattcggg ccattggacc atcgaaggct
      241 gcgtcaccag ccagttcgag aaccacctgc gcgccatttg cgatctaccg cttggcagca
      301 cgcgcacctt gggacacgcg gccatgatca actttttagg aacgatgccc gaccgcaagc
      361 gcctgttgca gatcgacggc ctggcgttcc acgattacgg caaggaagcg cggccgggcc
      421 gaaaactcgg ccattgcacc attctcaagg cgggcatcgc ggatcgcaac cgggcgctgc
      481 tgcacgctct aaatttgatc aaacccgcag actgagcacg aaatgtatct agaactcttc
      541 aaactccgcg agctgccgtt ccgcttgagc ccggacccgc agttccttta tctcagcaaa
      601 cagcacgcgc gcgccaaagc gtacatggag tcgacgatct ggttcaccga cgggttcgtg
      661 gtgatcacgg gcgaaatcgg ttccggcaaa accacgctga tcgaaaccat cctgcgcgaa
      721 ttgcaaggca acgtggtcgt tgcacagata aaccagactc aattgtcgcc gaccgagttt
      781 ctgcagacga tcctggtgca attcggtttc acccccttcg acatgaagaa gccggaggtc
      841 ttggcgacct tgaaccaatt cttggtggaa cagcacgtca acggccggaa ggtcctgttg
      901 atcatcgatg aggcgcaaaa tttgagcaac cgggtgctcg aggagctgcg catgctgtcg
      961 ggcatcgaga ccaccaagga taaagtcctc cgcatcatct tggcgggtca accggaactc
     1021 aacgacaagc tggattcccg cgagttgatc cagcttgcgc agcgcgttcg gctgcgcttt
     1081 cacttgagca cgctgtccca ggccgagacc agcgcgtata tcgatcatcg actcgaagtc
     1141 gcgggctcgc agggacgcag aattttcgag gaagacacct accccgtgat ctacaaatac
     1201 acgggtggcg tgccgcgatt gatcaatacg ctatgcgaca cggccatgat ggccgccttc
     1261 agccgggaca gggataccgt ggcccttcac gatctcgagg ccgcgatcga ggaattgcaa
     1321 tgggttgagt tcaacgccaa cacggatcgc atgagagtgc taagaatgga gcacgcgcct
     1381 cctccccatg tgaaaaatgg tcccgtcgtc ggccggattc tgctggcaag cggcggcaag
     1441 acggtgatgg agcgcgaact tcggcccggt cgcttggtga ttgggcgtac cgcggataac
     1501 gacatacaaa tcgacagtaa attcatcagc cggcatcatt gccaagttgt gaccctgcgc
     1561 gattcgtgcc tcatcgagga tctaaacagc accaacggca tctacgtgca atcgaagcgc
     1621 gttcgacgat acaatttgaa cgacggcgac gtggttcacc tcggtcagca cgaaatcatg
     1681 tatatcgatg aacgcgcacc gcggctgcgc gacggcaccg atccgtccgg cgccaatgat
     1741 gcggaaggcc actgaggagc ggcggcggac taccggttga gagtcggcaa gtcgatggac
     1801 tcgccaggat attcgcggct atgagcgcgg caatcctgcc gatgcagaaa cacggtgagc
     1861 gccgcgatct cggccagcaa tccaatcccg agcaccatca acgcgtacgg accccgggga
     1921 tcggcacata cgagcagaat cgccagcgcg ccgatcacca cgtaaatcac cggcaggatt
     1981 tcatagactg gcttagcaag gcgcatggat gagaacctcc atgccccata gtaatgccgc
     2041 agccgccgcg gctgcggccg attttactta aggcgtcggc ggtttactca cccgcggatg
     2101 cgcgggacgg cctattcccg gccgaccagc agctccgaaa gacgcgacaa ccaggaattg
     2161 cgccaacctt cgatctgcaa ctgctccttg ctgcgcttcg actggcgcgc cgactctgat
     2221 aaaagctcct tcggcagatt gagctgaaac gtaccggtca ttgatttgtc cgacatggaa
     2281 tctccatcga ggatgacact gggaaacaga gtatcggtac cggcggtgcc cgggatgcga
     2341 cgcgccgcac gctattgtgt tccatccgcg aatcgcggcc tatgcgtcgc caataagcga
     2401 cctggccgcg gaaatatttt tgccataata cgcgaccccc gcaactacga cgccgctcca
     2461 ttaccactgg gctttcttcg gcaaatcgtc gttgatctgg taatagtccg atttgtcggc
     2521 gacgaatagg tgctcgctca gggcgaggcg cgtcggcgca tcgagggttc ccgccgttac
     2581 ggagatcgtg tccttgccga attcactcca gaacaaattg ccgccgcaca cgcggcagaa
     2641 cccgcgctcg gcagtatccg aggatttgta ccagctcaag gtgtgggacg agaccagcga
     2701 taaattcgca gccggcacgc tggtcatggc ggcatggtgc ccgctggttt tccggcattg
     2761 catgcaatgg caggcaatca ccccgcgcag cgcaccctga atgcggtagc ggactccgcc
     2821 gcagagacag ccgccggcgt gc
//
LOCUS       DATMGX010000715         2020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_76028, whole genome shotgun sequence.
ACCESSION   DATMGX010000715 DATMGX010000000
VERSION     DATMGX010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76028"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /gene="ccsA"
                     /locus_tag="VNR65_11775"
     CDS             <1..369
                     /gene="ccsA"
                     /locus_tag="VNR65_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWJ19393.1"
                     /translation="FLYLGLIALRQAIDEPGRAGRSAAILALIGAVNIPIIKYSVNWW
                     NTLHQPASVIRADGPTIAPSLLWPLLIMALAFTILFVVLHLKAMRAEVLRRRCDAMIA
                     VHVRGDSAQEPAAGEAISHV"
     gene            362..544
                     /gene="ccmD"
                     /locus_tag="VNR65_11780"
     CDS             362..544
                     /gene="ccmD"
                     /locus_tag="VNR65_11780"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF016856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HWJ19394.1"
                     /translation="MFDLGPHAGFIWLSYLAAALCVTGLVVWVWTDERGQRRRLADLE
                     RRGMRRRSSQQNQDRP"
     gene            541..1161
                     /locus_tag="VNR65_11785"
     CDS             541..1161
                     /locus_tag="VNR65_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HWJ19395.1"
                     /translation="MSETISNQETPAKKKSRAVLLLPLVLVVALLGLMYFALQSGDPS
                     RLPSVLIGKPVPEFNLAGVPRLQNDQGAPVPGFSSAALKNGKVSLINVWASWCAPCLA
                     EHPQLVDLAKQGVPLYGINYKGDSAEPARRFLTKHGNPFVAVGVDETGMTAIDLGVYG
                     VPETFIIDGSGHIVLRYPGPLTPEVIAEKIKPAMEKAAKENATPRS"
     gene            1355..1636
                     /locus_tag="VNR65_11790"
     CDS             1355..1636
                     /locus_tag="VNR65_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19396.1"
                     /translation="MMFRSMLALLLILTGVPLIVAALNGDPTFIETQDARLHGLYAGV
                     VLLIAGFIIGKFWSLKWKMGRSATAAGATEQRQEPVEEQIDLFEPRNKA"
     gene            complement(1644..1958)
                     /locus_tag="VNR65_11795"
     CDS             complement(1644..1958)
                     /locus_tag="VNR65_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19397.1"
                     /translation="MLFLRLLGKTLLLAAFAALAYDGARILASPGEGLLFTSLSTHLK
                     HIPQGRELLESFFLNYAPPSLWNGIIEPMLVLPVSILFGAAGALIFLAGYRRPPPEIV
                     RE"
BASE COUNT          407 a          594 c          597 g          422 t
ORIGIN      
        1 ttcctttatc tcgggctgat cgccttgcgg caggctatcg atgaaccggg gcgcgccggg
       61 cgttctgccg cgatcctggc cctcatcgga gccgtcaata ttcctatcat caaatattcg
      121 gtgaactggt ggaacacgct gcaccagccg gcgagcgtga tccgtgctga cggcccgacc
      181 atcgctccgt ctcttctttg gccgctcctc atcatggcat tggccttcac gatccttttc
      241 gtcgtgctgc atttgaaggc catgcgcgcc gaagttttga gacgcagatg cgacgccatg
      301 atcgccgtcc atgtgcgcgg cgactccgcc caagagcccg cagcagggga ggcgatatcc
      361 catgtttgat ctcggaccgc atgccggttt catctggctt tcctatcttg cagccgcgct
      421 ctgcgtaacc ggtctcgtcg tgtgggtttg gaccgacgag cgaggccaga ggcggcggct
      481 tgcggacttg gaacgccgcg gaatgcgccg gagatcgtca cagcaaaatc aggacaggcc
      541 atgagcgaga caatctcgaa ccaggaaacg ccggccaaga agaaatcgag ggccgtgctc
      601 ctcctgccgc ttgtccttgt ggtcgccctg ctcggcctca tgtacttcgc acttcaatca
      661 ggcgatccat cgcgccttcc ctcggtcctg atcggaaagc cggtcccaga gttcaacctc
      721 gcgggagtcc ctcgccttca gaatgaccaa ggcgcgccgg tgccggggtt ttcctctgcg
      781 gcgctgaaga acggcaaagt cagccttatc aacgtctggg cgtcctggtg cgcgccgtgc
      841 cttgccgagc acccgcagct tgtcgacctg gcgaagcagg gcgtacctct ctatggcatc
      901 aactacaaag gcgattcggc tgaacccgcg aggcggtttc tgaccaagca tggcaatcca
      961 tttgttgccg tgggcgttga cgagacgggc atgactgcga tcgatttggg ggtgtacggc
     1021 gttcctgaaa ccttcatcat tgacggctcc gggcacatcg ttctgcgcta tcccggccct
     1081 ttgaccccgg aggttatcgc cgagaaaatc aagcctgcga tggagaaggc agcgaaggag
     1141 aatgctacgc cccgctctta gggctggtct aaacgcgtgt tgcagccgcc gggtgagctt
     1201 gcggccagat tttcggcggt tgcgcagcac ggctgtcgga tctgcgtcga ttagacaaag
     1261 ctaagatcga aagcgcagtt tgaccctcgc gcggcacaga actacgatcc gcacaatcgg
     1321 ttggacttca gccgatccgc aagaaaaggg ttatatgatg ttcaggtcca tgctcgccct
     1381 tctgttgatt ttgacaggcg tcccattgat tgtcgcagcg ctgaatggcg accctacgtt
     1441 cattgaaact caagatgcga gattgcatgg cctctatgcc ggcgtcgtcc tccttattgc
     1501 gggcttcatc atcggcaagt tctggtcgct gaagtggaaa atggggcgtt cagcaacggc
     1561 ggccggagcg acggagcaga ggcaagagcc ggtagaggaa cagatcgacc tcttcgagcc
     1621 tcggaacaag gcgtaagctg cctttattcc cttacgattt ccggtggggg acgcctatac
     1681 cctgcaagga aaatgagagc cccagccgct ccgaacagga tcgagacagg aagcacgagc
     1741 atcggttcta tgatgccgtt ccacagagag ggcggcgcgt aattcaagaa gaagctctcc
     1801 aacagctcgc ggccctgcgg gatatgtttc aagtgcgtcg aaagagaagt gaaaagcagc
     1861 ccttcccctg ggctggctaa aatccgcgcg ccgtcatagg ccaaagctgc aaacgccgcg
     1921 agaagcagcg tctttcccaa gagccgtaga aatagcatcg ctcactcccg ctgtggcgtt
     1981 gacttcctta gctgcgtccg gaaaagcgcg aagcggtttt
//
LOCUS       DATMGX010000716         2711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_80651, whole genome shotgun sequence.
ACCESSION   DATMGX010000716 DATMGX010000000
VERSION     DATMGX010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80651"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..435)
                     /locus_tag="VNR65_11800"
     CDS             complement(292..435)
                     /locus_tag="VNR65_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19398.1"
                     /translation="MARISDMHKKWMKEPKYRKAYQALEEEFVLASAVIEARKKRKSA
                     CEN"
     gene            complement(514..1704)
                     /gene="ftsA"
                     /locus_tag="VNR65_11805"
     CDS             complement(514..1704)
                     /gene="ftsA"
                     /locus_tag="VNR65_11805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWJ19399.1"
                     /translation="MIDVGSAKTCVIVAEVNDYALRYRGHGISDSRGMRKGVIADLDK
                     AVDSIRKAVEDAENVAQAPVEHAVVGVAGSHIRGVNSHGGISLGSRSREISREDVRQA
                     VEKARSIAMPDDREALHLLPQEFILDGQGGVHDAAGLMGRKLEVRVHVVTAGTTATQN
                     VVSAMNRAGIHVDDTVFEALACADSVLQTDERELGVCLVDIGAGSTDLIVYYEGVVIH
                     TGAIPIGGDHFTNDVGVGFRTPIPDAENMKKMFGCAIVTRIPEGNEIEVPSVGDRPSR
                     LMQQRMLGEILEPRARELMELLRDHLRQAGVYDLLGAGLVFTGGGSRLSSLLEVADDI
                     LRRPVRLGYPVPIAKLPSNLLEPEFATAIGMVLYGHRARQARMNLDRGLGAKLKALFA
                     RQNA"
     gene            complement(1738..>2711)
                     /locus_tag="VNR65_11810"
     CDS             complement(1738..>2711)
                     /locus_tag="VNR65_11810"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWJ19400.1"
                     /translation="LDARMLDLDDEAESPFLRGQKRVPVRRGALPRKAASRIKIALIL
                     ILVLASVTLIWIRLHQYATRSWRFRIDSSDNIQVSGSSNVSRYQVMEVMASDLNRNVF
                     FVPLELRKQQLEQIPWVQSASVMRLLPDRLNIVIKERTPVAFVEVNSHIELIDANGVV
                     MDLPASQQARYSFPVVVGMPDTEPVSTRAARMKIYAQLMKELDAGGAHYSRDLSDVDL
                     SDPDDVKATVTDANGAVLVHLGSSNFLERFRIYVAHVQEWRAQFQKLESVDLRYEHQV
                     IVNPDSASGKAKVGAAPGNGASNIDAPAPPATVQSNSAVKHKTKKHS"
BASE COUNT          528 a          863 c          740 g          580 t
ORIGIN      
        1 agcgttgccg ccaccaccgc cgacgccaat gaccttgatc ttcgcgctat tcagtagctc
       61 ttcgttgaag tgaatgcgaa tgtcgttttc gggctggctc ttgtcgttga aatcttcgat
      121 catctttact ccaatatcgc gactggcaat tcgcgattgt ttgtgtcgcc ggctgaagcc
      181 ggctcagata atgtcttcgt tcctacccag ggcttacgcc cctgggctaa gagcaattcc
      241 gcccctgcgg ggctcgactt tgcgcaaccg ttccagcctg gcaacccaaa ctcaattctc
      301 acatgcgctc ttacgctttt ttcgcgcctc gataacggcc gatgccagga cgaactcttc
      361 ctccagggcc tggtacgcct tccggtattt cggttccttc atccatttct tgtgcatgtc
      421 ggatatgcgc gccattgcca gacctccatc atgcaaccga tttcgcgcgt gtcaaatccg
      481 aattctgcgc tgcttttcgg cctcgctgga acttcaggcg ttctgcctgg cgaacaatgc
      541 cttgagtttt gctccaagtc ctcgatcaag attcattcgc gcctgccgcg cgcgatgtcc
      601 gtagagcacc atcccgatcg ccgtcgcaaa ctccggctcc aacagatttg aaggcagctt
      661 ggcgatgggg accggatatc ccagtcgtac cggacgccgc aaaatgtcgt cggcaacctc
      721 caggagcgaa ctgagccgcg acccgccgcc ggtaaacacc agccccgcgc ccagcaaatc
      781 gtaaacgccc gcctggcgca ggtggtcgcg gagcaattcc atcaactcgc gcgctcgagg
      841 ctccaggatt tcgcccaaca tgcgctgctg cataaggcgc gacgggcgat cgcccaccga
      901 aggcacttcg atttcattgc cctccggaat gcgggtgacg atagcgcagc cgaacatctt
      961 cttcatgttt tcggcgtcag gaatgggtgt gcggaagccc actcccacat cgttggtgaa
     1021 atgatcgcca ccgatcggga tggcgccggt gtggatcacg accccctcgt agtacacgat
     1081 gaggtcggtg gaacccgcgc cgatgtcaac caggcaaacg ccgagttcac gctcgtccgt
     1141 ctgcagcacc gaatcggcgc aagccagcgc ttcaaagaca gtgtcgtcga cgtggatacc
     1201 ggcgcggttc atggccgaga ccacgttctg ggtcgcggtg gttcccgcag taaccacatg
     1261 aacgcgcacc tcgagctttc tgcccatcag cccggcggca tcgtggaccc cgccttgccc
     1321 atccaggatg aactcctgcg ggagcaggtg cagcgcctcg cggtcatcgg gcatggcgat
     1381 actacgcgcc ttctccaccg cctgacgcac atcttcccgg ctgatctcgc gcgaacgcga
     1441 ccccagcgaa atgccgccat gactattcac gccgcggata tgcgagccgg caactccgac
     1501 cacggcgtgc tccaccggag cttgcgccac gttctcggcg tcttcgaccg ctttcctgat
     1561 cgagtcgacc gccttgtcga gatcggcaat cacgcccttt cgcataccgc gcgaatcgga
     1621 gatgccgtgg ccgcgataac gcaaggcata gtcattcact tcggcgacga taacgcacgt
     1681 cttggcgcta ccaacatcaa tcactgcaag caggttttcc gtttgacttc ccatggttta
     1741 cgagtgcttt ttcgttttgt gcttcaccgc tgagttgctt tgtacggtcg cgggcggtgc
     1801 cggcgcgtct atgttactag ccccgttccc cggcgcggca ccaaccttgg ctttaccgct
     1861 tgcggaatcg ggattaacaa tcacctggtg ttcgtaacgc aaatccaccg attccagttt
     1921 ctggaactgc gctctccact cctgcacgtg ggccacgtag attctgaaac gctccagaaa
     1981 gttcgacgac cccagatgca ccagcacagc cccgttggca tcggtgacgg tcgccttcac
     2041 gtcgtcagga tcggaaagat cgacgtcgct gaggtcgcgc gagtaatgcg cgccaccggc
     2101 atcgagttcc ttcatcaact gagcgtagat cttcattcgc gctgcgcgag tcgagacggg
     2161 ctcggtgtcg ggcatcccca ccaccacagg gaaggaatat ctcgcctgct ggcttgcggg
     2221 aagatccatg accacgccgt tggcgtcaat cagttcaatg tgggagttga cttcgacaaa
     2281 tgcgaccggt gtacgctctt tgataacaat gttcagacgg tcggggagca gccgcatcac
     2341 cgaagccgat tgcacccagg gaatctgctc caactgctgt ttgcgtaact ccagcggaac
     2401 aaaaaaaacg ttgcggttga ggtcgctcgc cattacttcc atgacctggt agcggctgac
     2461 attgctgctc cccgagacct gaatgttatc gctggaatcg atgcgaaagc gccaggaccg
     2521 cgtagcgtat tgatggagcc taatccagat cagggtaacg ctcgccaaga ccaggatcag
     2581 gatgagcgcg atcttgatgc ggctggcagc cttgcgcggc agcgctccgc gacgtaccgg
     2641 aacgcgcttc tgcccccgta aaaatgggga ctccgcctcg tcatcgaggt cgagcatgcg
     2701 ggcatcgagc g
//
LOCUS       DATMGX010000717         2538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_292628, whole genome shotgun sequence.
ACCESSION   DATMGX010000717 DATMGX010000000
VERSION     DATMGX010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292628"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VNR65_11815"
     CDS             <1..245
                     /locus_tag="VNR65_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19401.1"
                     /translation="SPVLFWQERPGLGGRPIRLYKFRDGDGAGLAEAILELAGNPELA
                     AEQGRRARRLFETKYEFAHAVAAWERLIQKVSVRDF"
     gene            complement(524..1042)
                     /locus_tag="VNR65_11820"
     CDS             complement(524..1042)
                     /locus_tag="VNR65_11820"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HWJ19402.1"
                     /translation="METRSFAHAPNWMVLTTHAHREEFAIENLVRQDYEAYCPMIVKR
                     IKHARRAYDAKRPLFPGYVFVERPAQRHLWRPLLGTFGVRSVVCNGETPSLLPAGFVE
                     SLRARETDGAIRKPEMPFKTGQSVTINGGAFDGFVGQILEIRESDRVLLLLNLLNQQT
                     RVHIETKMLRSA"
     gene            complement(1144..2211)
                     /gene="gmd"
                     /locus_tag="VNR65_11825"
     CDS             complement(1144..2211)
                     /gene="gmd"
                     /locus_tag="VNR65_11825"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWJ19403.1"
                     /translation="MEKRALITGVTGQDGAYLTDLLLKKGYRVWGIKRRSSSFNTGRV
                     DSFYQDPHTGDPRFRLVHGDMTDATNLIRVVQEVQPDEIYNLAAQSHVQVSFETPEYT
                     ANADAIGPLRLLEAIRILGLQKKVRFYQASTSELYGKVAEVPQSEKTPFYPRSPYAAA
                     KIYAYWITVNYREAYGMHASNGILFNHESPLRGETFVTRKITRAVASISLGLQDKLYL
                     GNLDAKRDWGHARDYVKGMWAIVQQDQPDDYVLATGETRTVREFVEAAFAVANYRIDW
                     KGEGVNETGVDAKTGRTLVEIDPKYFRPTEVDILLGDASKARDILGWKPTVHFVELVK
                     EMVAEDMKTVLLERERVERDV"
     gene            complement(2214..>2538)
                     /locus_tag="VNR65_11830"
     CDS             complement(2214..>2538)
                     /locus_tag="VNR65_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HWJ19404.1"
                     /translation="PRRELLFVDDLADSCIFLMRAYSGPVALNVGTGEDLTIRSIAEV
                     MRSVVGYEGELAYDPSYPDGTPRKVLDVRRLSALGWKAKTSLHEGLTQTYRWYCDNLT
                     QLRAA"
BASE COUNT          580 a          714 c          687 g          557 t
ORIGIN      
        1 gttccccggt cttgttctgg caggagcgcc cagggctcgg tggcagaccc atcaggctgt
       61 ataaattccg cgacggcgat ggtgctgggc ttgccgaggc gatccttgaa cttgccggca
      121 atccggagtt ggccgccgag caaggccgtc gcgcaagacg gctttttgaa acgaaatacg
      181 agtttgctca cgctgtcgca gcttgggaac gtctgatcca gaaagtttcc gttcgcgact
      241 tttagttttc tttcgctcaa tggccacgca acaacttcca ggacaggcag cggcatttct
      301 tcctgcgcgc catctcaaga atggcttgct tcggtgagtt tgtggtcacg ccccatagca
      361 tccctgcggc ttcgagcgca cccactgatg tctccaaagc ggattaatgg cttcagaaaa
      421 taacagccgc acgtccacgg aaacccgcgc caaacgagtc agcgattcaa gcggttggca
      481 ttattcaaga gcgattgcga tctgaatgct gagatagccc gtttcacgcc gagcgcagca
      541 tcttcgtttc gatatggacg cgtgtttgct ggttaagaag attgagaagc agcaggacgc
      601 gatcactttc ccggatttcc aggatttggc ccacaaagcc gtcgaaggca cccccattga
      661 tggtgacgga ttgacccgtc ttaaatggca tttcgggttt tcgaatcgcg ccatccgttt
      721 cgcgcgccct taagctttca acgaaaccgg cagggagcag ggacggtgtc tccccattgc
      781 aaacaaccga gcgtactccg aaagtgccga gcagagggcg ccagagatgg cgctgagcgg
      841 gacgctcaac gaagacatat cccggaaaaa gcggccgctt cgcatcataa gcccggcggg
      901 catgcttgat gcgctttaca atcatcgggc agtaggcttc gtagtcctgc cggaccaaat
      961 tttcgatcgc aaactcttca cgatgagcat gggtggtgag aaccatccaa tttggagcat
     1021 gagcaaatga acgtgtttcc atgacactga aacctcacaa tatcaacaac aatagttttt
     1081 gcggcagagc atcggtttga ttgccccgac ctcgcggaaa cagtcaggag cgggcgataa
     1141 ctctcacaca tcccgctcga cgcgttcccg ttcgagaaga acggtcttca tatcctcggc
     1201 aaccatttcc ttcacaagct ctacaaaatg gaccgtaggc ttccagccga gaatatcgcg
     1261 ggcctttgag gcatccccaa ggagtatatc aacttcagta ggccggaaat atttcgggtc
     1321 gatttccacg agcgtccgac cggttttggc atcgacgccc gtttcgttaa ccccctcgcc
     1381 tttccagtcg atgcgataat tggccaccgc aaaggccgcc tcaacgaatt ctcgaaccgt
     1441 gcgggtttcg cctgtggcca gaacataatc gtcgggctgg tcttgctgca caatggccca
     1501 catgcctttg acgtaatccc tcgcgtgtcc ccagtcgcgt ttggcgtcca ggttcccaag
     1561 gtagagctta tcctgcagcc cgagcgagat ggaagcaaca gcgcgcgtaa ttttcctggt
     1621 tacgaaggtt tcgccgcgca aggggctttc gtgattgaag agaatgccgt tggaggcatg
     1681 cattccatac gcttcacggt agttcaccgt gatccagtac gcatagatct tggcggcggc
     1741 ataaggcgac cgcggataga aaggggtctt ctcgctttgc gggacctccg ccaccttgcc
     1801 atagagctcc gacgtcgagg cctgataaaa ccgcaccttc ttctgaaggc ccaggatccg
     1861 aatggcttca agaaggcgca acgggccaat tgcatccgca ttggccgtgt attcgggggt
     1921 ctcgaaactc acctggacgt ggctttgggc cgcaagatta tagatctcat caggctgaac
     1981 ctcctggaca acgcgaatga ggttcgtggc atctgtcatg tcaccatgga cgagcctgaa
     2041 acgtggatca ccggtatgcg gatcctgata aaatgagtcg acgcgcccgg tattgaacga
     2101 ggatgacctt cgcttaatgc cccaaacccg atagcctttt ttcaaaagaa ggtcggtcaa
     2161 ataggcgccg tcctggccgg taacgccagt gatcaatgct ctcttttcca taatcaagcc
     2221 gctctaagtt gtgtgagatt gtcgcaatac cagcgataag tttgggtgag gccctcgtgg
     2281 agagatgttt tcgctttcca gcccaaggcg gaaagccgac ggacatcaag cactttgcgc
     2341 ggcgttccat cggggtacga cggatcatag gcgagttcgc cctcgtaacc gacgacggac
     2401 cgcataacct cggcaataga ccggatcgtc aaatcctccc cggttccaac attgagcgca
     2461 accggcccgc tataggcccg catgagaaaa atgcaggaat ctgccagatc gtctacaaaa
     2521 agaagctcgc ggcgcggc
//
LOCUS       DATMGX010000718         2752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_421239, whole genome shotgun sequence.
ACCESSION   DATMGX010000718 DATMGX010000000
VERSION     DATMGX010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421239"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VNR65_11835"
     CDS             complement(<1..230)
                     /locus_tag="VNR65_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19405.1"
                     /translation="MTEFKADPSKGVFDSSETKNLSEAFEKADEIINRPETPLDSEID
                     RDDLARHIISEALEGETQPDLLSRSAVAKMLI"
     gene            462..650
                     /locus_tag="VNR65_11840"
     CDS             462..650
                     /locus_tag="VNR65_11840"
                     /inference="COORDINATES: protein motif:HMM:NF022890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HWJ19406.1"
                     /translation="MPAKSKAQQMAAGAALAAKRGEAKKSELKGASKSMVESMTEREL
                     QDLASTKRKGKPDHVKAS"
     gene            complement(1168..2544)
                     /locus_tag="VNR65_11845"
                     /pseudo
     CDS             complement(1168..2544)
                     /locus_tag="VNR65_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          533 a          802 c          835 g          582 t
ORIGIN      
        1 tcaatcaaca tcttggcgac ggcggatcgc gaaagcaggt ccggctgagt ttcgccttcg
       61 agcgcttccg aaataatgtg gcgcgcaaga tcgtcccggt caatctccga gtcgagcggg
      121 gtctcgggac gatttataat ctcgtcagct ttctcgaacg cctcggacag atttttcgtt
      181 tccgatgaat cgaacacgcc tttggaaggg tcggctttga attcggtcat tgtcacgctc
      241 tcgagttgct ggagttttga cgcgctgaac ctggcgtccg cccccgtact gaggcggacg
      301 gcatatgggt ctgatctcaa gaactaccag cggccggatc ctcgatacct gagccgtcgc
      361 gatggccgga ccccgcgctg aaccttctga cgtcgcggca gttaggcttg gcgtgatgat
      421 taacgcacgg aactgtggcg cgaacgtgaa aggagaggaa tatgccggcg aagtccaaag
      481 cccagcaaat ggccgctggc gcggctttgg ccgcaaagcg cggcgaagcc aaaaagagcg
      541 agcttaaagg cgcatcgaaa tcgatggtcg agtctatgac cgaaagagag cttcaggatc
      601 tggcctcgac gaagcgaaag ggtaagccgg atcatgtcaa agcctcttag tgctgtgctg
      661 aagatcaaac ccgaggagag aatgtgatgc accctcaagc gatgattaac acccatcccg
      721 cgggtcggac acgcgacctg gatgcgctgg tgagatgcgt tgaggcctgc tttgactgcg
      781 ctcagtgctg cacagcttgc gcggacgcct gtctcgggga agacatggtt gcggagttgc
      841 gcgcctgcat ccggttgaat ctcgattgcg ccggtatctg cgccgcaacc ggcgccatcg
      901 catcgagaca gacctttacc aacgaggcag tcctgggcgt ggcgctagag gcttgcgcgg
      961 aaatttgccg ggcctgcgca gcagagtgcc gccagcattc cgagcgtcat gaacattgcc
     1021 gggtctgcgc cgaggcatgc gaggactgtg atcaggcctg tttgcacgca atacaatctg
     1081 caggcgttca ctagccgcaa aggggcattg gctgtggcag tcggccgcga ttcgcaacgc
     1141 gttttgtcgg ttatcgaaga tcgatgatat gttatacgcc agaacgcgaa ggctcatctc
     1201 agttcgcacc ttttcgaggg tcttggtgag gaagtgcgcg cttcccgtcc atgatttcag
     1261 ggtgccgaag ggatgctcga cggtctgcct gcggatcagc atggcgtctg gccgatgctc
     1321 aaggcgggct tgcattttat cgagcacgcc ttcgtgacgc cagcgcttga gcctcttgag
     1381 tttgtccggg gtgcacttcg gcctgagggc gcaagtgaag caggcggtga gatgacggta
     1441 atggtcgatg tcgtcctttc gatctgatcg cacgagcccc ttggtcagcc tttgtcctgc
     1501 cgggcagatg tagtgatcct tctcagcatc gtagatgaag tcatggcggg ggaagaaacc
     1561 ctcttcgctc gtccacacgg ttttggggat acagggaagg atgcctgttc cttcacacgc
     1621 cagcacctgc tcgccattga agtaaccgcg atccgccaac gccgtgagct ctccgcaccc
     1681 ggtcgccgcc agcgccttca gcgccatggg cttcaactgg gttctgtcgt ggcccaggtt
     1741 ggtcacctcg tgcgcgacga tcagatggtg ctccgcatcg accgcggtct gcacgttgta
     1801 gccgaccatg cccgtgcccc ggccgcttgt agccattgaa cgggcgtcgg gatcggtcag
     1861 cgacacctgt ttgtcaggcg catcctgaac cttttgttcc atcgccttca gagcctgcat
     1921 ctgccgccgc aggccggcga tcttgtcctt gatgcggcgg gtcctggctt cggcgatgtc
     1981 gctctcttcg cggtcagctc gatccagggt cgtcagatag cgtgcgatgc tggcgtccac
     2041 ctgctccatg cgcttggcga ccttagtcag cgtaaagttc ttgtctcggt tgttgaccgc
     2101 tttgaacttg ctgccgtcga tggccacgat cgccttggtg aacaggttga gccggcggca
     2161 aagctcgatg aactgaccgc acgccgctct gatggccgga ccattgtcgc gacggaagtt
     2221 ggcgatggtc ttgaagtccg gcgccaggcg tccggtgagc cacatcagct cgatattgcg
     2281 ctgagcctcc cgttcaaggc ggcggctgga ctggatccga ttgagatagc cgtagaggta
     2341 gatcttcagc agtgtcgacg gatgataggc gggccgaccc gtcgccgccg ggtgaacgcc
     2401 ctcaaagcca agcgcgccga gatcaagctc ttcaataaag acttcgacca cccgaacagg
     2461 gttgtcctcg gtcacgtaat cgtcgagcgt ctccggcaat aacaacgact gccgccggtc
     2521 ttcaccctcg acgaaacgcc ccatgaccgg ccctccgatg tcgagtcgat tctagcaaat
     2581 tcgaaggcct ttttacacag tctggaccca accacgtcgc tcaggcgcgg atcagctcat
     2641 cccgaaaccg gtcttagcga gggcgaaccg accgacggta gagcgcccat ctacgtcgtt
     2701 cagtcactga tcagctcatc ttgaaaccag ccgcagaccg aaacgactga cg
//
LOCUS       DATMGX010000719         3403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_358343, whole genome shotgun sequence.
ACCESSION   DATMGX010000719 DATMGX010000000
VERSION     DATMGX010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358343"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VNR65_11850"
     CDS             <1..1403
                     /locus_tag="VNR65_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWJ19407.1"
                     /translation="ESEERLRAVIETAVDAIVVIDEKGLIQSINPATERIFGYAPNDV
                     VGKNVSILMPDRDAAVHDNFIAAYLRTGKAKIIGFGREVDHRRKDGSVFAADLAVAEW
                     RVGGKRYFTGNIRDITERKQYEKELQLLLREVNHRAKNMLAVVQAIARQTAAGTRDEF
                     MERFGERVRALGVSQDLLVKNEWRGVDLNELIRSQLAHFKDVIGTRIEIRGAPLFITA
                     SAAQTIGMAIHELATNAGKYGALSNHEGRVEVDWSLEHSEKGSKTLVMSWVELSGEVV
                     TAPTEQGFGTTVIVPMARMSLDAEVDLDYETTGLIWRLRCPAENVLEGNRSPATAKTL
                     SPDDERAGSGARPTILVVEDEVLIALDIKQILADAGFDIVGPARSAAQALDLIGRDGC
                     EAAVLDVNLGCETSEPVALELKERGLPFVALSGYSREQHPAAFTGAPALTKPLIPDLL
                     VTELRRLLETESRTNR"
     gene            complement(1669..3027)
                     /locus_tag="VNR65_11855"
     CDS             complement(1669..3027)
                     /locus_tag="VNR65_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HWJ19408.1"
                     /translation="MSNFRPIDRQTAYLLPPSVDDWLPEEHLARFVVDVVEQLDLSAM
                     SKAYRGSGSASYHPCLLVGLLVYGYATGVFSSRKLERATYDSIAFRFVAANQNPDHDT
                     IAAFRRRFLKEVEALFVKILLLAREMGLLKLGTVGLDGTKIHANASKHSALSYEHAAK
                     IEAKLKAEVAELLAKAEAADQADVPDGMSIPEELARREERLKKIAEAKAKIEARAKER
                     YERELAEHEAKLAARAAKEKATGKKPGGKPPAPPAEGPLPSDQVNLTDEESRIMPVSG
                     GGFEQCYNAQAAVAEGSLLVVANGVTQAPNDKRELEPMLAKTSALPEELGKTKTLLGD
                     NGYFSEANVKACEAAGVEPLLAMGRDSHHPSLSERFAGAPPAPANPTPVEAMAHRLRT
                     PEGKALYAKRKCTPEPVFGIIKSALGFRQFLLRGLENVRGEWSLVTMAWNVKRMFSLK
                     TA"
BASE COUNT          681 a         1046 c         1024 g          652 t
ORIGIN      
        1 gcgagagcga ggagcgtctt cgcgcggtca tcgagaccgc tgtcgacgcg atcgtcgtga
       61 ttgacgaaaa aggcctgatc cagtccatca atccggccac ggagcgcata ttcggctacg
      121 cgccgaacga cgttgtcggc aagaacgtct ccatactcat gcccgatcga gacgctgcgg
      181 tgcatgacaa cttcatcgcc gcctacctcc gtacgggaaa agccaagatc atcggcttcg
      241 ggcgcgaggt ggatcaccgc cgcaaggacg gctccgtttt cgccgctgac ctcgccgtcg
      301 cggaatggcg tgtcggcggc aagcgttatt tcaccggcaa cattcgcgac atcaccgagc
      361 gcaaacagta cgagaaggag cttcagcttc tactgcgcga ggtcaaccac cgcgccaaga
      421 acatgctcgc tgttgttcag gccattgccc ggcaaaccgc cgcggggacg cgtgatgaat
      481 tcatggaacg ttttggggaa cgggttcggg cgcttggcgt cagccaggac ctgctggtca
      541 aaaacgagtg gagaggcgtc gatctcaatg agctgatccg gtcgcagctc gcgcatttca
      601 aggacgtgat tggcacgcga attgagatca gaggcgcgcc gctctttatc actgcatctg
      661 ctgctcagac catcggtatg gcaatccacg agcttgccac caatgccggc aagtatggtg
      721 ccctttccaa ccatgaggga cgcgtagagg tcgattggag ccttgagcat tccgaaaagg
      781 gcagcaagac cttggtgatg agttgggtcg agcttagcgg agaggttgtt acggcgccga
      841 cagagcaagg ctttggcacc acggtgatcg tccccatggc caggatgagc ctcgatgccg
      901 aggtcgacct cgattacgaa acaaccgggt tgatttggcg gcttcgatgc cccgccgaaa
      961 atgtcctgga gggcaacagg tctcccgcca ccgcgaaaac tttgagtccc gacgatgagc
     1021 gcgccggatc gggcgcgcgc ccgaccattc ttgttgtgga agacgaagtc cttattgcgc
     1081 tcgatatcaa gcaaatcctc gcagacgcgg ggtttgacat tgtcggaccg gcgagatcgg
     1141 cggcccaggc ccttgacctt ataggccgag atggatgcga ggctgccgtg ctcgacgtca
     1201 acctcggctg tgagacatca gaacccgtgg ctctcgagtt gaaggagcgc ggcttgccct
     1261 tcgtcgcgct gtcgggctat tcgcgcgagc aacacccagc cgcattcacc ggtgcgccgg
     1321 cgcttacaaa accgctcata ccggaccttc ttgttacgga actcaggcgt ctgcttgaaa
     1381 cggaaagccg cacgaaccga tgagcgctgt ctagactgga gcaagcgctg agtgatgcaa
     1441 ttcctgccag aaagtgcgtt cgatacgccg cccatagcat tttgcagcac ttgacgcaca
     1501 taactctgga acaggtacgc gcatgcggct catctcgccc cctggtcgga ctgtaaatgc
     1561 ggggacggct ctagcagcct gtcggactgg gggtgggagc agcggcatca tgacagcgcc
     1621 ttgtagcgga gcttgcgcgt caggccaaca cagcggatga accgcccctc aggcggtctt
     1681 gagagagaac atccgcttca cattccaagc catggtcaca aggctccact cgccgcgcac
     1741 attttcgagc ccgcgcaaga gaaactggcg gaatccgagc gcggacttga tgatgccgaa
     1801 gaccggctcg ggcgtgcatt tgcgcttggc gtagagcgct ttgccttcgg gcgttcgcag
     1861 gcggtgagcc atggcttcaa caggtgttgg atttgccggc gcgggcggcg cgccggcaaa
     1921 gcgctcgctc aaggacggat gatgcgaatc ccggcccatg gcgagcagcg gctcgacccc
     1981 tgccgcctcg cacgccttta cgttggcctc gctgaagtaa ccgttgtcgc cgagcagcgt
     2041 tttagtcttg cccaactctt cgggcagggc gcttgtcttg gcgagcatcg gttcgagctc
     2101 acgcttatcg ttcggggcct gcgtcacacc gttggcgacg accaggaggc tgccttccgc
     2161 gacagcggct tgcgcgttgt agcattgctc gaagccgccg cctgaaaccg gcatgatgcg
     2221 cgactcttca tcggtcaaat tgacctgatc gcttggcagc ggcccttccg ccggcggcgc
     2281 gggcggcttg ccgccaggct ttttgcctgt cgccttctcc ttggccgcgc gggccgcaag
     2341 cttggcttcg tgctcggcca gttcgcgctc atagcgctct ttggcgcgcg cttcgatctt
     2401 ggctttggct tcggcgattt tcttcagccg ctcttcccgg cgcgccaact cctccgggat
     2461 cgacatccca tcgggaacat ccgcctgatc ggcggcttcg gccttcgcca aaagctccgc
     2521 aacctcggcc ttcaacttcg cctcgatttt ggccgcatgc tcataagaga gcgcgctgtg
     2581 cttgctggcg ttggcgtgga tcttcgtccc gtccaagcct accgtcccca acttcaagag
     2641 gcccatctcg cgcgccaaaa gcaaaatctt cacgaacagc gcctcgacct ccttcaaaaa
     2701 gcgcctacgg aaggcggcga tcgtgtcgtg atcgggattc tggttggccg caacgaagcg
     2761 gaaggcgatc gagtcgtacg tcgcccgctc cagcttgcgg ctggagaaca cgcccgtggc
     2821 gtagccgtaa accagaagcc caaccaacag acaaggatgg tagctggccg aacccgagcc
     2881 gcgataggct ttgctcatcg ctgagaggtc cagctgttcg acgacatcca ccacaaaccg
     2941 cgccagatgc tcttccggca gccagtcatc gacggacggc ggcagcaaat aggcagtctg
     3001 ccggtcgatc gggcggaaat tgctcatgaa ggcgcagcca agcttgagga atcaatcgca
     3061 agagattgcc ctattcaaat ggcgaggtta agtccgacag gctgctagaa caaatgaagg
     3121 cttacgccga gcagacgcag gagcttcaaa agctgatcgg ggaaactctc cagaagctcc
     3181 aacacggcta atgcgcggag gcatgcacaa gctaatcgtg tcgctgcgag ggcgttccct
     3241 ctccgccgcc caccagccca atggctcagc caaaacctgc accgcccgaa ggatgtgctg
     3301 cacttacggg atgaccaggg ccgtcgcaaa cctcatctca aacagcaatg ggtgattccg
     3361 cccgaggcca atgcggcctt tgtcgctgcc atggaggacg tgc
//
LOCUS       DATMGX010000720         7088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_39289, whole genome shotgun sequence.
ACCESSION   DATMGX010000720 DATMGX010000000
VERSION     DATMGX010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39289"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VNR65_11860"
     CDS             <1..457
                     /locus_tag="VNR65_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HWJ19409.1"
                     /translation="TMGAKALLIHPSYQGVCWALVLSSPAMAADYLGKVTAVADGDTF
                     TMESETGRVRVRICGIDAPERGQPGYGQAAGALSNMIEGKLVHCLQVGEGTVCDGKSK
                     PTSRDRIVAQCFLDKLDIAEEMTRSGTVCDWPKFSGGHYKISDNTCSRK"
     gene            485..772
                     /locus_tag="VNR65_11865"
     CDS             485..772
                     /locus_tag="VNR65_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19410.1"
                     /translation="MCNLYSMTRNVDAIRRLFKVDAAHDRTGNLPPLPGIFPDYPAPI
                     VCKDGKERELAMARWGMPSSQKAQLDAAKKCVQKLDVNRRGVPTPIGTLSY"
     gene            867..2423
                     /locus_tag="VNR65_11870"
     CDS             867..2423
                     /locus_tag="VNR65_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HWJ19411.1"
                     /translation="MRAGIRISSLVPSGLVIESVSDSSDSIILAVRSEGGVAECPLCG
                     ARSRRIHSRYDRQVADLPCAGKQIRLRVITRRFVCEVPHCRRQIFAERFGDDVLPTWS
                     RRTARLESIVHHLGLALGGRPAASFAKRLMLPVSNDTLLRVVRRRTASRADPLTVVGV
                     DDWAFRRNRRYGTIVCDLERRRIVTLLPDREISTVRAWLADHPGIKIVSRDRGGGYGE
                     AAAKALPNAVQVADRWHLMENASAAFLDAVRKSMRAIRSAIGTTTITPELLTSAEKLR
                     YESYLRREDTNTAIMTLARDGVSIKQIVRRLGHSRNLVRQILRGGRTDMYQTRQRSLD
                     AYYLFLEGQWSGGCRNGAELWRRLQARGFRGSLRSVGEWTTRRRRSEAVSKQQLQRIP
                     SARTIARLMTIRRDCMTKADAVVIAAVEAGVPSLVEARTLIDQFHDMIRKKDEAKLDA
                     WIAHAKASLVSSLATGVLKDRAAVRAAITQPWSNGQVEAQITKLKLVKRRMYGRAKID
                     LLEARLLGAT"
     gene            complement(2519..4429)
                     /locus_tag="VNR65_11875"
     CDS             complement(2519..4429)
                     /locus_tag="VNR65_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HWJ19412.1"
                     /translation="MTAVERGPEMSTAETVAAETAPQHGALGTMAIGALGVVYGDIGT
                     SPLYTFKTAIEWAGGEATPAVALGILSLVVWTLLITTSLKYVAIVMRADNDGEGGILA
                     LMSLLGIKRGERRFVIALGIFGAAMLYGDGAITPAISVLSALEGLKDPLPSFAPYVLP
                     LSVIVLIAVFALQPQGTARIGALFGPVMTIWFVTIGVLGLAGILRHPGVLAALDPRYG
                     LGYLFGHGFTGFLVLGGVFLCATGAEALYADMGHFGPRPIRLTWYGLVLPTLVLNYAG
                     QTAVLLDGGATSGSNPFFTLCPASLQLPLVALATVATIIASQAIISGTFSMTRQAIQL
                     GLCPRLHITQTSSQGYGQIYVGFVNWTLMLLTLALTVSFRSSDNLASAFGIAVALTML
                     LTSVLMFLAMRELWGWSLWASVAVAGLFVIVDLSFVAANMMKVMEGGWVPLVVGATLF
                     FLMSTWHRGRIALGEKLERDTLPLTDFIGQMRAKARVQGTAVYMTSRLDMVPVPLLHN
                     LKHNKALHQRIVLLHVVTASIPRVPPDRRLVLRDLGDNFFTIVAEYGFMQQPNVPRVL
                     HRCAARGLAFDMMETSFFVGRVTIVPARHSSFGRLRCVLFEIMHRNALTATDFFRIPP
                     NRVIELGSQTEI"
     gene            complement(4575..4772)
                     /locus_tag="VNR65_11880"
     CDS             complement(4575..4772)
                     /locus_tag="VNR65_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19413.1"
                     /translation="MGLRLWVFVAGVAGLAHLILYIADREVNASVDRLSQLRLARPGE
                     LDNDSNLKLVSARSPYMRNER"
     gene            complement(5168..5377)
                     /locus_tag="VNR65_11885"
     CDS             complement(5168..5377)
                     /locus_tag="VNR65_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19414.1"
                     /translation="MLTHTRGPSEGARYSAGSRDPARADVAAAYLAMIKRLFMRVLMI
                     LAAAAALAAIMALKFAIYLPRFIHH"
     gene            complement(5459..5941)
                     /locus_tag="VNR65_11890"
     CDS             complement(5459..5941)
                     /locus_tag="VNR65_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19415.1"
                     /translation="MIAVQLTSPDLSRRQALGRSTAAIERESAGMLPADGPATVTAQF
                     QPLDPGIVNEAIPAFFIGRNMKGFWVARDVKGQIGGIFLLENSAVSFARRNSRPAGCA
                     TIFPSERIELDLENRGNPFVVQLGSWRRVARRRRERLAVLVGKMTEAVKRRLKDLRVL
                     "
     gene            complement(5938..6132)
                     /locus_tag="VNR65_11895"
     CDS             complement(5938..6132)
                     /locus_tag="VNR65_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19416.1"
                     /translation="MAAITFNAAHQKVHIREQLKAVLAAFREMLDVFVSSRMRRAVAE
                     AEHIRPRRPHGTQSPSINAQ"
     gene            6378..6695
                     /locus_tag="VNR65_11900"
     CDS             6378..6695
                     /locus_tag="VNR65_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19417.1"
                     /translation="MAHFLTSDDKYRDLIHDDDYRSYLINRTDSPIGYRFGKTHGKRV
                     RVHARTALSRVNGYLKNMIEAIANAKLRRMERELELRGIRFDRPNNNWVARNPGRTSV
                     RED"
BASE COUNT         1486 a         2102 c         2154 g         1346 t
ORIGIN      
        1 cactatgggg gcgaaggcgt tactcattca cccatcgtac caaggtgttt gctgggcatt
       61 agtcctgtcg tctccggcga tggccgccga ttacttgggc aaggtaaccg cggtcgcgga
      121 cggcgacacc tttacgatgg aatcggagac ggggcgggtg cgcgttcgca tctgcgggat
      181 cgacgctccg gagcgaggcc agcccggcta cggccaggct gcaggagcgc tttccaacat
      241 gatcgaggga aagctcgtcc actgcctgca ggtgggcgaa ggcaccgtgt gcgacggcaa
      301 gtcgaaaccg accagccgcg accgcatcgt cgcgcaatgc tttctcgaca agctcgacat
      361 cgccgaggaa atgaccaggt cgggcacggt ctgcgattgg ccgaagttct ccggtggtca
      421 ttacaagatc agcgacaaca cgtgttcgcg gaaatagcgt ccgtcgggac gccgggcagt
      481 tttcatgtgc aatctctact ccatgacccg caacgtcgac gcgatccggc ggctgttcaa
      541 ggtcgatgcg gcgcacgatc gcaccggcaa ccttccgcct ttgccgggca tctttccgga
      601 ctacccggcg ccgatcgtgt gcaaggacgg caaagagcgc gagcttgcga tggcgcgctg
      661 gggcatgcct tcttcgcaga aggcgcaact ggatgccgcc aagaagtgcg tccaaaaact
      721 cgatgtcaat cggcgcgggg tcccgacgcc gatcggcacg ctaagctatt gatccaacta
      781 gcggtggagg gggcaatctt ggggtctgac tcacatctga tgattttgct gccgagcttg
      841 ggttctccaa cgggggaatc agcgccatgc gagccggtat tcgaatctcg tcgcttgtgc
      901 cgagtgggtt ggttatcgag agcgtaagtg attcatctga ctcgattatt ttggccgtcc
      961 ggtccgaagg cggtgtggcc gagtgcccgt tgtgcggggc gagatcacgc cgaatccata
     1021 gccgatacga cagacaggtc gcggatttgc cctgtgcagg caagcaaata cgcctccgcg
     1081 tgatcacgcg gcggtttgtt tgcgaggtac ctcattgtcg gcggcagatt ttcgctgaac
     1141 ggttcgggga cgatgttctc ccgacctggt cgcgccggac ggcacggctg gaatccatcg
     1201 tccaccatct ggggctggca ctcggcggca ggccggcagc gagtttcgca aaacggctga
     1261 tgctgccggt tagcaacgat acgttgcttc gcgttgtgcg acggcggacg gcgtcacgag
     1321 ccgatccttt gaccgtcgtc ggtgtcgacg attgggcctt tcgacgcaat cgccgctacg
     1381 gaacgatcgt ctgcgatctc gagcggcgac ggatcgtgac gctgttgcca gatcgcgaga
     1441 tttcgacggt tcgagcctgg ctcgccgatc atcctgggat caagatcgtg tcccgtgatc
     1501 gcggcggcgg ctatggcgag gcggccgcca aggcattgcc caacgccgtt caggtcgccg
     1561 atcgctggca tctaatggag aacgccagtg cggccttcct cgacgcggtg cgcaagtcca
     1621 tgcgcgcaat ccgcagcgcg atcggtacga caacaattac cccagagctg ctcacctcgg
     1681 cagaaaagct gcgatacgag agctatctgc gacgcgagga tacaaatacg gcaatcatga
     1741 cgctcgcgag ggacggcgtg tcgatcaagc agattgtccg ccgtctgggc catagtcgca
     1801 atctcgttcg ccagatcttg cgtggcggtc gaaccgacat gtaccaaacg cgtcagcgct
     1861 cgctcgacgc atattacctg ttccttgagg gccagtggag cggtggctgt cgcaacggcg
     1921 ccgaactctg gcgtcgcttg caggcgcgag ggttccgggg atccctgcgt tcagtcggcg
     1981 agtggacaac acgtcgacgt cggtcggaag cggtcagcaa acaacagctg caaagaattc
     2041 cgtcggccag aactatcgcc cgtctcatga cgattagaag agattgcatg accaaggctg
     2101 atgccgtggt catcgccgcc gtcgaggctg gtgtgccgtc ccttgttgag gcccgcacgc
     2161 tcatcgacca attccacgac atgatccgaa agaaggacga ggccaaactc gacgcctgga
     2221 ttgcgcatgc aaaggcaagc ctcgtctcat ccctcgccac gggagtcctc aaggacagag
     2281 ccgccgtgcg tgctgcgatc acgcagcctt ggtcaaacgg ccaggtcgaa gcacagatta
     2341 cgaaactcaa actcgtcaaa cggcgaatgt acgggcgcgc gaaaattgat cttctcgagg
     2401 cacgattgct cggcgccacc tgatctgcga cgtcgtcatc aaatatgcgt cagagccaat
     2461 cttggacgct tattcacaga atggtcggcg ccgcggtcgc gcaaagttct atcccaacct
     2521 agatttccgt ctggctgccg agctcgatga cgcgattcgg cgggatgcga aagaaatcgg
     2581 tcgccgtcag ggcgttgcgg tgcatgatct cgaacaatac gcaacgcaag cgcccgaagc
     2641 tcgaatggcg ggccgggacg atcgtcactc gtccgacgaa gaacgaggtc tccatcatgt
     2701 cgaatgcgag accccgcgcg gcgcaccggt gcaacacccg gggtacgttc ggctgctgca
     2761 tgaacccgta ctcggcaaca atggtgaaga agttgtcccc gagatccctc agtacaaggc
     2821 gccggtcggg gggcacgcgc gggatactgg cggtgacgac atgcaacaga acgatgcgct
     2881 ggtgcagcgc tttgttgtgc ttcagattgt gcaacagcgg caccggcacc atgtcgagcc
     2941 ggctcgtcat atagaccgcg gtcccttgga cacgcgcctt ggcgcgcatc tgaccgatga
     3001 agtcggtgag cggtagcgtg tcgcgctcga gcttttcgcc aagcgcgatg cggccgcgat
     3061 gccaggtgga catcaggaag aagagcgtcg cgccgaccac cagcggtacc cagccccctt
     3121 ccatgacctt catcatgttg gcagccacga aagacagatc gacgattacg aacaggccag
     3181 caaccgcaac gcttgcccaa agactccagc cccacagctc gcgcatcgcc aggaacatca
     3241 gtaccgacgt cagcaacatg gtcagggcaa cggcgatgcc gaaggcggac gcaagattgt
     3301 ccgacgagcg gaagctgacc gtcagcgcga gtgtcaacag catcagggtc cagttgacga
     3361 agccgacata aatctggccg tagccttgcg aggaggtttg ggtgatgtga aggcgcgggc
     3421 acagtccgag ctgaatggcc tgccgcgtca tcgaaaacgt gcctgagatg attgcctggc
     3481 tcgcgatgat ggttgccact gtcgcgagcg cgaccagcgg cagttgcagg cttgccggac
     3541 atagcgtgaa gaacggattc gatccggatg tggccccacc gtccagcagc acggcggttt
     3601 ggccggcgta attgaggacc aaggtcggca gcacgagacc ataccatgtc agccggatcg
     3661 gtcgcgggcc gaaatggccc atatcggcat agagcgcttc ggcgccagtg gcgcacagga
     3721 acacaccacc gaggacaagg aagccggtga agccgtgtcc gaacagataa ccgaggccgt
     3781 agcgcggatc gagcgctgcg agtaccccgg gatgacggag gatgcccgcc agtccaagca
     3841 cgccgatggt aacgaaccag atcgtcatga caggcccgaa cagcgcgccg atccgtgcgg
     3901 tgccctgcgg ttgcaacgcg aacaccgcga tcagtacgat gaccgacagg ggcagcacgt
     3961 agggcgcgaa ggaaggcagg ggatccttca ggccttccag cgcactcagc accgatattg
     4021 caggcgtaat cgcgccgtcg ccgtagagca tcgccgcacc gaaaattccg agcgcgatca
     4081 caaatcggcg ctcgccgcgc ttgatgccga gcagcgacat cagcgccagt atcccgccct
     4141 cgccgtcgtt gtcggcgcgc ataacgatcg cgacgtattt cagcgaagtg gtgatgagca
     4201 gcgtccatac cacgagcgac aggatgccca gggcgacggc cggcgtcgcc tcgccgccgg
     4261 cccattctat cgcggtcttg aaggtgtaga gcgggctggt gccgatgtcg ccatagacga
     4321 ccccgagcgc cccgatcgcc atcgtcccta acgcaccatg ctgcggagcg gtctcggccg
     4381 ctaccgtctc ggcggttgac atctcaggcc cgcgttcgac ggcggtcatc gtccgttccg
     4441 gcttgtcaat agcgcctcgc tggcttgggt ggccggagtg tgcttggtcg gaccggcaga
     4501 tgcaactgag catgggccaa tcgtagcggc ggccggtccc acagccttac tgaactctcg
     4561 ggatatcgct ggttttatcg ctcgttgcgc atatacggac tgcgcgcgct cacgagcttc
     4621 aggttggaat cgttatccaa ctcgccggga cgcgcaagcc tcaattggga gagccggtcc
     4681 acacttgcat tgacctcacg atccgcaatg tagagaatca ggtgagcgag ccctgccaca
     4741 ccggcgacaa aaacccaaag acgcaagccc atataaagtc tccacggttt gccaccggca
     4801 tgtccaaagc atgatcggct cccgtcgcga tgcagctcta tctgcggagt ctgctgcgaa
     4861 aaagccccga gcgaccgcgg gagtcgaccg caatctcgga cgttggaacg gtcatagccg
     4921 aacgcgtcga gatcagaacg agtcgctgag ccgaaggcct agcttggaat tgaggtgaaa
     4981 aacaaaatgg ccatcatcgc gacgaccaaa gcaaccttca gttcaaccat atcatcgaac
     5041 cccagtcccg atcggtgacg agaaagaact ttctcgtaat cgcaatcgca agtgttgatt
     5101 catgtcaacg ctcgagcctt cgttcgcgag atcgggatca aaaaggccat cctgcactga
     5161 gcgaaagtca atgatgaata aagcgcggaa gatagattgc gaactttagc gccatgatgg
     5221 cagcaagggc agctgccgcg gccaggatca tcaatacgcg catgaaaagc cgtttgatca
     5281 tcgccagata ggctgccgcc acatcggctc tggccggatc tcggcttccc gctgagtagc
     5341 gggccccttc ggaggggccg cgggtgtgcg tcaacatcgt tatctctaaa atcctttact
     5401 ggctgttcgc aagggctgag cgacgcagcc tgtgtcgccg tcctgcgccc gcttgacctc
     5461 agaggacgcg aaggtccttc agccgccgtt tgaccgcctc tgtcattttg ccaacgagga
     5521 cggccaatcg ctcccgtcgg cgcctcgcca cgcgcctcca cgatccaagt tgcacgacaa
     5581 acgggttgcc tctgttttcc aggtcgagtt cgatcctctc ggatggaaat attgttgcac
     5641 accctgccgg ccggctattc cttctggcaa acgacacggc ggagttctca agcaggaaaa
     5701 ttccgccgat ctgccccttg acgtcacggg caacccagaa gcctttcatg ttgcggccaa
     5761 tgaaaaatgc cgggatcgcc tcgttgacga tgccgggatc gagaggctga aattgcgcgg
     5821 tgacggttgc gggcccatcg gccggcaaca tgccggcgct ttcccgctcg atcgcagcgg
     5881 tcgaacgtcc cagcgcctgc cgccgtgaaa ggtctgggct tgttagttgc acggcgatca
     5941 ttgcgcgttt atcgatggcg attgcgtgcc atgggggcgc cgcggacgaa tgtgctcggc
     6001 ctcggctacc gcccgtcgca tccggctact gacaaagaca tccagcattt ctcggaaagc
     6061 ggcgagaacg gctttgagct gttctcgaat gtgcaccttc tgatgtgcgg cattgaaagt
     6121 aatcgcggcc atggcggcct cctcacgaat ctaacgtgca tcatgtgccg acgatgccat
     6181 aggaatgcga gatggatcgg cgtttagtct cataggagag atataagaat cccgggggag
     6241 atgatgccgc tctcatctgc tccaggtctt tataccgcgc ctatgagaga gggcaggagg
     6301 tcgcctcgat ttcctatact gtcaggcgca tcttcggacc aatagctcac aatatgagct
     6361 gactgtggag acggaagatg gctcactttc ttaccagcga cgataagtat cgcgatctga
     6421 tccacgacga tgattatcga tcgtatctta tcaaccgaac agactcaccg atcggttacc
     6481 ggttcggcaa gacgcatggg aagcgcgttc gcgtccacgc acgcaccgct ttaagccgcg
     6541 tcaacggcta tttgaaaaat atgatcgagg ctatcgccaa tgcgaagctg cggcgcatgg
     6601 agcgcgagct cgagcttcgc ggcattcgct tcgaccggcc caacaataat tgggtggcgc
     6661 gaaatcccgg tcgaaccagc gttcgcgagg attgatacgg tccatacccg acccgcagtc
     6721 cgggttcagt tcttcccgtg gagcatcaca tgcgacagct ttcgttcacc ggatctgttc
     6781 ttgtttccct tctgcgcagc gccgtcagct ctctgctgac gtttctgagt ccatcctggg
     6841 acggctcggt caggaaatac cgcccggagc aacattatat gcgtggtccc ggaccgaaat
     6901 ggcgcgaaaa gcgttataaa ggtgcaagcg ccacgtgacg ttgagggcat tctcacaaag
     6961 gtacctcggt ccgaaggcaa ttgaaggcaa cacagagaat caagcgatgc agatggactg
     7021 gctgacctcc tttggccttt tggctgttgc ctccatgctc gcttgctatg cattggaaga
     7081 ttgcagcg
//
LOCUS       DATMGX010000721         3027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_39478, whole genome shotgun sequence.
ACCESSION   DATMGX010000721 DATMGX010000000
VERSION     DATMGX010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39478"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VNR65_11905"
     CDS             <1..404
                     /locus_tag="VNR65_11905"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ19418.1"
                     /translation="AAELATFFEDLPYRMSNSHLIVSRSGATTVAELCALGRPAILVP
                     LPNAKDNDQLENATRMESAGAGYCYPQGSLTAEGLAGAVRRLFEDPAALVRAASAAHS
                     LANYRAVENLANLLEELAEKQGSAVGEEVTA"
     gene            468..1901
                     /gene="murC"
                     /locus_tag="VNR65_11910"
     CDS             468..1901
                     /gene="murC"
                     /locus_tag="VNR65_11910"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWJ19419.1"
                     /translation="MKDPLSGGRVHIIGIGGIGMSAIAEIMAARGLEVQGSDLKEGAN
                     LRRLSSKGIKVFVGQCAENLEDATTVVISTAVKDTNPELQAAREKGLPVFSRADMLAA
                     LMKGYRTISVTGSHGKTTTTAMIGWMMTEAGLDPTVLVGGVVRAWGSNARIGSSKWMV
                     VEADESDGTFIRLPTEIGVVTNIDPEHLDYYGTEAALHEAFLTFFRQIPKDGVVVPGI
                     DHPVVRSLLNRVDAEGYLPPMAAYGRALDAQTRIDDIVTNGGSVTFDTILAAGAGHHP
                     LRRDKFKLRVPGAHNALNAAAAITVGQHIGLTPDQIFEALSTFEGVDRRFTSTGEWNG
                     VSIYDDYAHHPTEIAAALSAARGAAKGHVIAVMQPHRYSRLKSLFDQFCECFKEADMV
                     LIAPVYAAGEAPNGFDRDRLVEGVRHTGHARVYPVEGEDTLTRTIAALARPGDLVIGL
                     GAGTISEWTHALPGRLSLYSAFAGAAE"
     gene            1904..2827
                     /gene="murB"
                     /locus_tag="VNR65_11915"
     CDS             1904..2827
                     /gene="murB"
                     /locus_tag="VNR65_11915"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWJ19420.1"
                     /translation="MFPDLVPSLLAAAPDLRGRLLPNVLLSDLTWLRVGGPAQVLFTP
                     ADEGDLAYFLSRCPLDIPVMVVGVGSNLLVRDGGVPGVVIRMARGFNEGLPVSATRIR
                     AGAALPDVRLAQIAASAGIAGLAFYRGIPGTVGGALRMNAGAHGGETKDVVTEARAVD
                     RSGKIHVFDNVGMGFTYRHCSVPGDHIFTHAIFEGREGESAEIEGEMKGVAEYRETHQ
                     PIKSRTGGSTFKNPPGQSAWKLIDAAGCRGLCMGGAHMSEMHCNFLINDQGATAADVE
                     QLGELVRERVFAQSGIMLEWEIKRIGVPAAQ"
BASE COUNT          585 a          903 c          929 g          610 t
ORIGIN      
        1 tggctgccga gcttgccaca ttcttcgagg atcttcccta caggatgtcc aattcacact
       61 tgatcgtgtc gcgaagcggc gccaccacag tcgctgaact ctgcgcgctc ggccgccccg
      121 ccatactggt tccgcttccg aatgccaagg acaacgacca gctcgaaaac gcaacccgga
      181 tggagagcgc gggcgccggc tattgctacc cgcaagggtc gttgaccgca gagggacttg
      241 ccggagcggt gcgccgtctc ttcgaggatc ctgctgcgct cgtgagggcc gcatcggctg
      301 cgcattctct cgcgaactac agagctgttg aaaatcttgc gaatctgctt gaagagttag
      361 cggaaaagca aggttcggct gtgggcgaag aagtaactgc ttaggaatca gcttctatat
      421 taggacaagc tgcccgcagg cagttaaggg gaagagagga cacggcgatg aaagatccgc
      481 tttccggcgg tcgcgttcat atcattggca ttggcggcat cggcatgagc gccatcgccg
      541 agattatggc ggcgcgtggc cttgaggttc aaggcagcga cctgaaagag ggagcaaatc
      601 ttcgccgcct ctccagcaaa ggcatcaagg ttttcgttgg ccagtgcgcc gagaatctcg
      661 aagacgcaac aacggtcgtt atttcgaccg cggtcaagga cacaaatccg gagcttcaag
      721 ctgcgcgcga gaagggtctg cccgtgttca gccgggctga tatgctcgct gcgctgatga
      781 agggttaccg gacgatctcg gttacagggt ctcacgggaa gaccacgacc acagccatga
      841 tcggctggat gatgaccgag gccggtctcg atccaaccgt tctcgtcggc ggcgtggttc
      901 gcgcttgggg atcgaatgcg cggatcggct cgagcaaatg gatggtcgtc gaagcggatg
      961 agtcggacgg cactttcatc cggctgccga ccgaaatcgg cgtcgtgaca aacatcgacc
     1021 cggagcatct cgattattat gggacggagg ccgccctgca cgaggcgttc ctgaccttct
     1081 tccgtcaaat tccgaaggac ggcgttgtcg tacccggtat tgatcatccc gttgtgcgca
     1141 gccttctcaa tcgggtcgac gcggaaggat acctcccacc gatggcagcc tatgggcgcg
     1201 ctctggatgc gcagacacgg atcgacgata tcgtgacgaa tggcggatcg gtaacctttg
     1261 acacgatcct tgctgcaggc gcaggtcacc atcccctccg gagagacaaa ttcaagcttc
     1321 gggttccagg cgcgcacaac gcgctgaacg cggcagcagc gatcacagtg ggacagcata
     1381 tcggccttac gcccgatcag atttttgaag ccctctcgac ctttgaaggc gtcgaccgca
     1441 ggttcacctc gaccggagag tggaacggcg tctcgattta cgacgattat gcccaccacc
     1501 ccacggaaat agcggccgcg ttaagcgccg cgcgtggcgc tgcgaagggc cacgtcattg
     1561 ccgtcatgca gccgcaccgc tattcgaggc tgaaatcgct attcgaccag ttttgcgaat
     1621 gcttcaagga ggccgacatg gtgctcatcg cgccggtcta tgccgctggc gaagcgccaa
     1681 atggttttga tcgcgacagg ctcgtcgagg gcgtcaggca tacgggacac gcccgcgtct
     1741 atcccgttga aggcgaggac acgctcacgc gcacgattgc tgctctcgca cggccgggcg
     1801 atcttgtgat cgggcttggc gctggcacca tcagtgaatg gacgcacgcc cttccgggcc
     1861 gtctcagcct atattccgcg tttgccggcg cggcggagta atcgtgtttc cggaccttgt
     1921 tcccagtctt ctggcggcag cgcctgacct tcgcggcagg ctcctgccga atgtcttgct
     1981 cagcgatctg acatggttgc gcgttggcgg tccggcacag gtcctcttca cgccagccga
     2041 cgagggcgat ctcgcttatt tcctctcgcg ctgcccgctc gacattccgg tgatggttgt
     2101 cggcgtcggc tcgaacctgc ttgtgcgcga cggcggcgtt cccggtgtcg tcattcgcat
     2161 ggctcgtggc ttcaacgagg ggctgcccgt cagcgccacg cgaattcgcg cgggtgcggc
     2221 tctgcccgat gtcaggctcg cccagatcgc tgcatcggca ggcatcgcag ggctcgcctt
     2281 ttatcgcggt attccgggaa cagtcggcgg cgcgctgcgc atgaacgctg gcgcgcatgg
     2341 cggcgaaacg aaggatgtcg ttacggaggc gcgcgcagtc gaccggagcg gcaagatcca
     2401 cgtcttcgac aacgtcggga tgggcttcac ctaccgccat tgcagcgttc cgggcgatca
     2461 cattttcacc cacgccattt ttgaaggccg cgaaggcgag tccgccgaaa tcgaaggcga
     2521 gatgaaaggc gtcgccgagt atcgcgaaac gcatcagccc ataaaaagcc gcacaggggg
     2581 ttcaaccttc aaaaatccgc cgggccaaag cgcctggaaa ctcattgatg cggctggctg
     2641 ccgcggtctt tgcatgggcg gcgcgcatat gtctgagatg cactgcaatt tcctcatcaa
     2701 cgatcaaggc gccaccgccg ctgatgttga gcagcttggt gagctagtcc gcgagcgtgt
     2761 cttcgcacag agcgggatca tgctcgaatg ggaaatcaag cgcatcggcg ttccggccgc
     2821 gcaataggac gttgctgctc cgggtgagca ctgctccatt cgaaatagga gcaacccatt
     2881 tcatttctag cgacataggc cggaacccgc ccttcaactt ctcagttgag gcgtctcttc
     2941 cttgggggaa gcgagcgcca gtttgccgcg agcgcgtgcg acctgcttcg taagttcttt
     3001 cgccctatcc ggcgggttcc ttaaaat
//
LOCUS       DATMGX010000722         2401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_228776, whole genome shotgun sequence.
ACCESSION   DATMGX010000722 DATMGX010000000
VERSION     DATMGX010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228776"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1571
                     /locus_tag="VNR65_11920"
                     /pseudo
     CDS             141..1571
                     /locus_tag="VNR65_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_054926070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(1869..>2401)
                     /locus_tag="VNR65_11925"
     CDS             complement(1869..>2401)
                     /locus_tag="VNR65_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HWJ19421.1"
                     /translation="TATTFGLVSRYAHWITATLMLCLVPMGLFVSVLKDESPDRAEFL
                     AAHQSLGLAVFGVVAVRLFWLLISPPPAPSPDLSPREHRLSRAVHIGLYGLILAFPLS
                     GFLMSAHGGDAIQFFGWALHSPIDPGKRAAAIFATTHDLVLPSLLYVLIFAHIGAVLK
                     HHFVDRKSSDVRRMLT"
BASE COUNT          557 a          679 c          723 g          442 t
ORIGIN      
        1 atgaggcggt attcgctggg cattgtaccc aacgaaaatt ggcgagacgc gcggttcagg
       61 ccgaggctgt gtgaaaagta cagctttcta agaaagtgat tctgcttcag aatctttcct
      121 gcccagagcg ggggatttgg atggcaagct ttgtaagcgg gatcgatcgc agccagggaa
      181 cgatgtttcc ggcgcagctg gaagattatg tcgccgaaga caacccggtg cgggtgatcg
      241 atctgtttgt cgatcagctt gatcttgcca agcttggttt cggcggcgtc actccgaagg
      301 acatggggcg gcccgcgtac catcccgcag tgatgctgaa aatctacgtt tacggctacc
      361 tcaaccgcgt tcaatcgagc cgccgcctcg agcgggaatg ccagcgcaat gtcgaggcga
      421 tgtggctgac gggctgcctg gctcccgatt tcaagaccat cgccgatttc cgcaaggaca
      481 acggtgcagc catccgtaaa gtttgccgcg agttcatcct ggtgtgcggg cgcgcggggc
      541 tgctcgccgc cacatcggtt gccatcgacg gcagcaagtt caaagcggtc aattcccgcg
      601 accgcaattt cacgcaaact aaggtggcca agcggctgga acagatcgag gcgagcatcg
      661 cgcgctacct ctccgaactt gaaaccgctg accgccagcc ctccacgccc gaaatcaaga
      721 tcgcccggct caacggcaag atcgccaagc tgaaacaaga aatcgaacgc atgaaagcga
      781 tcggggagca gctcgaaaaa tcagaagaca cgcagatttc gctgaccgat cccgatgcgc
      841 gctccatgca agggacgggc aaggcaactg gaaccgtggg ctataatgtg caatgcgcgg
      901 tggaaacgga gcaccacctc atcgtcgcgc atgaagttac caacgccgtc catgaccgcc
      961 atcagctctc cggcatggcc cagcaggcca aagaagccct tggcgccgaa gccatcgacg
     1021 ccctcgccga tcgcggcttc tacgatggca aagaaattct ggcttgcggg caagctggtg
     1081 tgacggctta tgtgccaagg ttttcgacct cgaacgccaa ggcggagggg cgtttcggca
     1141 agcaagattt cgtctacaac gccgatgaag atgtctatgt ttgccccaat ggcgagcgcc
     1201 tagcctaccg ttttacgagc gaagaagatg gtaaagtcat gcgctgttac tcgacgacgg
     1261 cctgctcagc ctgcccgctg aaagaacaat gcaccaccgg caaggagcgc cgcgttaagc
     1321 gctgggagca cgaggccgtt ctggatgccg cccaggagcg gctggataaa aatccagata
     1381 tgatgacggt gcggcgcagc acggtggagc atcccttcgg aacgctgaag ttctggatgg
     1441 gctcggcgca cttcctgatg aagaagctgc acaatgtgaa gacagaaatg tcgctccatg
     1501 tgcttgccta caacatgaag cgcgcgatga agatcctcgg cgtgcctgga ttgctgcagg
     1561 tgatgcaggc atggcgcata atttgttact ttttactcgt tttggcatgt gcggtgcgct
     1621 caaacagccc agatcgtggt ctgaaccgtt cccgcgcaat tctggcctgt ctcgcgaaat
     1681 ccgcgcctat gcatcaacgt ccctacgccg tggcctagca aatccgggtg aaacggactt
     1741 ttcccacagc ctcggcctat tcccgaagat aggctgctct ttttcaggga aggttgctgc
     1801 ctgtcgctca cgaagggagt cgttcgcaaa gttcgccttc atcctttggt gaaaccgaaa
     1861 cagccagctt aggtcagcat ccggcggaca tcactgctct tgcgatccac gaagtggtgc
     1921 ttcaggaccg cgccgatatg cgcgaaaatc aaaacataga gtaacgacgg cagaacgagg
     1981 tcatgggtgg tcgcgaagat cgccgcagcg cgcttgcccg ggtcgatggg agaatgcagc
     2041 gcccatccaa agaactgaat tgcgtcgccg ccatgagcgc tcataagaaa gccgctgaga
     2101 gggaacgcca aaatcaggcc gtagaggccg atgtgtaccg cgcggctgag gcgatgttcc
     2161 cgcggcgaca gatcgggcga aggggcaggc ggcgggctga tcaaaagcca gaacaagcga
     2221 acggccacga cgccaaacac ggccaagccg agcgactgat gggcggcaag gaactcggcc
     2281 cggtcggggg actcgtcctt cagcaccgat acaaacaggc ccatgggcac taggcagagc
     2341 atcagcgtcg cggtgatcca atgtgcatag cgcgaaacaa ggccgaaggt ggtcgccgta
     2401 t
//
LOCUS       DATMGX010000723         2366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_421602, whole genome shotgun sequence.
ACCESSION   DATMGX010000723 DATMGX010000000
VERSION     DATMGX010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421602"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VNR65_11930"
     CDS             complement(<1..1226)
                     /locus_tag="VNR65_11930"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucC family protein"
                     /protein_id="HWJ19422.1"
                     /translation="MSTLGHRVTGFWFRQGARFLPFADAATPELPLSRLLRLSLFQVS
                     VGMALVLIIGTLNRVMIVEYEVPASIVAIMISLPLFFAPFRAIIGFRSDTHKSVLGWK
                     RVPFIWQGTLVQFGGLAIMPFAVLVLAGQGEAEHLPAWIGQIAAGISFLLVGAGLHTV
                     QTVGLALATDLAPKESQPKVVGLMYVMLLFGMIASALLFGYLLLHPTWERLPPGQLIG
                     VIQGSAVATLILNTIALWKQEVRKPARLLTPEELAVETAPRPLFKESWAAYVHGGNAI
                     RRLVSVGLGTMAFSMEDVLLEPYGGQILHLSVSATTRLTATLAIGGLFGFALASRILS
                     RGFDPFKMALYGAIVGIPAFVFVILAAPFGSTVLFVLGTSLIGFGAGLFGHGTLTATM
                     SLAPKNQTGLALGAWGA"
     gene            complement(1688..1882)
                     /locus_tag="VNR65_11935"
     CDS             complement(1688..1882)
                     /locus_tag="VNR65_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting protein"
                     /protein_id="HWJ19423.1"
                     /translation="MADVDGQAGIWLVVPPKVGLPLFLGTVTLIAVLVHAALITHTAW
                     YPKYWEGGKKVAQIETTVVS"
     gene            complement(1909..2061)
                     /gene="pufB"
                     /locus_tag="VNR65_11940"
     CDS             complement(1909..2061)
                     /gene="pufB"
                     /locus_tag="VNR65_11940"
                     /inference="COORDINATES: protein motif:HMM:NF040862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting antenna LH1, beta subunit"
                     /protein_id="HWJ19424.1"
                     /translation="MADPNKVYPTGLTLAEAEELHGHVINGTRVFGAIAVVAHVLSYV
                     YSPWLH"
BASE COUNT          577 a          686 c          665 g          438 t
ORIGIN      
        1 gcgccccaag cgccgagcgc caaacccgtc tgatttttcg gcgcgaggct catcgtggcc
       61 gttaacgtcc catgcccgaa aagacctgcc ccaaaaccga taagcgacgt cccgaggaca
      121 aaaagtaccg ttgagccgaa cggcgccgcg agaatgacga atacgaacgc tgggataccc
      181 acgatcgctc cgtaaagcgc cattttgaat ggatcgaagc cgcggctcag aatgcgagag
      241 gcgagagcga agccgaagag accgccgatg gcgagagtcg ccgtcagcct cgttgtggcg
      301 ctaacgctga gatgcaggat ctggccgcca tacggttcga gcagcacgtc ctccatgctg
      361 aacgccatgg taccaaggcc gaccgaaacc aagcggcgaa ttgcgtttcc gccatgaacg
      421 taggcagccc aagactcttt gaacaagggg cgcggcgcag tctctacagc caactcttcc
      481 ggcgtcaaga gccgggcagg cttgcgcacc tcctgcttcc acaacgcaat tgtattcagg
      541 atgagagtcg cgacggccga cccctgtatc acaccgatca gctgcccggg gggaaggcgt
      601 tcccatgttg gatgcaagag cagatagcca aagaggagcg cgctggcgat catgccgaac
      661 agcagcatga catacatgag cccgacgacc ttgggctgcg actccttcgg cgccaagtcg
      721 gtggctagcg caagacccac agtttggacg gtatgcaagc ctgcgccgac aagaagaaag
      781 gatatgcctg ccgcgatctg cccgatccat gccgggagat gctccgcttc gccctgccca
      841 gccagaacga gcaccgcaaa cggcatgatc gcgagaccgc cgaactgaac cagcgtgccc
      901 tgccaaatga acggaacgcg cttccagccg agaaccgatt tgtgcgtgtc ggagcgaaag
      961 ccgatgatcg cgcggaaagg cgcaaagaaa agcggcaggg atatcatgat cgcgacgatt
     1021 gaagcaggca cttcgtattc aacgatcatg acgcggttga gagtgccgat gatcagcacc
     1081 agagccattc caaccgagac ctggaagaga gacaggcgca gcagcctcga cagaggcagc
     1141 tcaggcgtgg ccgcgtcagc gaagggaaga aaacgtgcac cttggcggaa ccagaaaccg
     1201 gtcactcggt ggccgagagt agacatcgat tgagcaactc caacagaaag ggcccggagg
     1261 ccacagtaga ccgtcgccga atagtgaccg aagccttagc ttttgtgccc cgtgaaagca
     1321 aaataagcgc tgctaggcaa ggtagcctca gcatcgcttt gcatccgtat tgggcttcag
     1381 accatagcat ctgatcacac cgatcagcac cctttggcat tgacctgccc gttcagggca
     1441 tcttccaagt tacctttacc tcagacatac aacgggaagc ttcacgcgat cgcctgcgcg
     1501 atagcaaaag ctttcagctc cgcgcaggct tcgacttccg gcacacggcg acccgctttt
     1561 gtcaagctgc tcactcatgt gtcacacccg tctcggctgt gctgccggaa gatatctgtg
     1621 ttccaccagt gtgtaggccc aggacctctt ggatccgggc cctctgacgg gctgtcgaaa
     1681 aagctactta gctaacgaca gttgtctcga tctgagcgac cttcttgccg ccttcccagt
     1741 acttcgggta ccaagcggtg tgcgtgatga gagcggcgtg cacgaggacg gcgatcagcg
     1801 tcacggtgcc cagaaagaga ggaagaccga ctttgggagg aacgaccaac caaatgccag
     1861 cttgtccatc aacatcagcc atgttcaatt ctcctagtta gagtgtgatt agtggagcca
     1921 aggggaatag acgtaggaca gaacgtgagc cacaacagca atcgcgccga aaacgcgcgt
     1981 gccattgatc acatgcccat gcaactcctc agcttccgcc aaggtcaaac cggtcggata
     2041 gaccttattt ggatcagcca taatgtacct cctaaaagga cctgctaggt ttttcgtgct
     2101 gaacccgcac gtaggtcgct cataacagct gctaattcga gcgagttttc ttcgtcgcag
     2161 cttgagtgta agcatatgct gacatttgca cgcgtcaact ccatttgaca cacctttgca
     2221 ttttaggtca atatgacgca aggttggttc tggctgaaaa ctgccggttt ccagcgtaaa
     2281 tggcaagaat agttcggagc gcttccaatt cgcccggttt tattggtatc cgggccagaa
     2341 aggacgcggc gggatcgtgc gccctg
//
LOCUS       DATMGX010000724         2010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_111807, whole genome shotgun sequence.
ACCESSION   DATMGX010000724 DATMGX010000000
VERSION     DATMGX010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111807"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..1765)
                     /locus_tag="VNR65_11945"
     CDS             complement(311..1765)
                     /locus_tag="VNR65_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWJ19425.1"
                     /translation="MNGYEVIHEKSPAFYDLYERKGELQQQTHYCPGCGHGIVHKLIA
                     EALTDLGLQDRTILVSPVGCSVFAYYYFDVGNVQVAHGRAPAAATALKRSHPESIVMA
                     YQGDGDLAAIGTAEIVHAANRGENISVFFVNNSVYGMTGGQMAPTSLMGEVTTTSPWG
                     RRASNEGLPLHVCELLAALETPTYIERVALNDMKNIMKAKRAVKKALEIQKNGGGFSL
                     VEILSPCPTIMKMDPVVARKWVGEALVKAFPLGVFRDHKPELPVGPEIPVKTVAQALE
                     VEEGVPEIASTRRHHTRDLTVKIAGFGGQGILLMGQLLAEMGLREHMEVSWLPSYGPE
                     MRSGSAHCHVSLSHERIGSPLISQPEVLIAMNELSLLKFAPSVVSGGLVLYNHTKLPA
                     GFSVPQARVVCVPASEIADRLGSAKVANVVMLAALLEETECLPTESAIAVLEATVKNP
                     KLLELDRQAMKAGRDYVDTEVEVGAVSQPDGYSY"
     gene            complement(1822..>2010)
                     /locus_tag="VNR65_11950"
     CDS             complement(1822..>2010)
                     /locus_tag="VNR65_11950"
                     /inference="COORDINATES: protein motif:HMM:NF028456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19426.1"
                     /translation="RKAAQHVKSVMVVELNTGMMLEDVKLTLNGTVPVEFYGRTGGNV
                     PAAEEITAQVFARMSALV"
BASE COUNT          380 a          657 c          560 g          413 t
ORIGIN      
        1 agccagcaat ttcgaattca aaaatcgctt ctggatatgt gcttcagcgc cgcactcgcc
       61 gcggccaaac gccccgctta tgccgcagct tcagagttag agcagattct gagttggcgt
      121 atccgggtgg agcaccgctt cagcggtgca tttgagagtt gcctctgaat ttggctttag
      181 ccgctgaggt gacttaagct cctaccgcag cggctaaagc ctcgcccttt ttctgcagcc
      241 tcggtatgca cgcctgaagg cgtgctccac ccagcacaag atacagaaac cctggaactg
      301 ccccagcttt tcagtacgag tacccgtcgg gctgcgatac cgcgcccact tcaacttccg
      361 tatccacgta atcgcgtccc gctttcatgg cttggcggtc gagttcgagc agcttgggat
      421 tcttcaccgt cgcctccagc accgcaatgg ctgactcggt gggcaaacac tcggtctctt
      481 ccagcagcgc cgcgagcatg accacattgg ccacctttgc cgaacccaag cggtcggcaa
      541 tctccgacgc cggtacgcac actacccgcg cctgcggcac gctgaaaccc gcgggcagtt
      601 tggtgtggtt gtagaggacc aatccgccgg aaaccacgct gggcgcgaac ttgagcagtg
      661 agagttcgtt catcgcaatc agcacttcgg gttgcgagat cagcggcgag ccgatgcgct
      721 catgcgacag cgaaacgtgg cagtgcgcgc tgccgctgcg catctccgga ccgtacgacg
      781 gcagccagct tacttccatg tgctcgcgca gacccatttc ggccagcaac tgtcccatca
      841 acaggatgcc ctgcccgccg aagccggcaa tctttaccgt caggtcgcgg gtgtgatggc
      901 ggcgtgtgga tgcgatttcc gggacgccct cctccacctc cagcgcctgg gcgacggtct
      961 tcaccgggat ttccggacca accggcaact ccggcttgtg gtcgcggaag acacccagcg
     1021 ggaaagcctt caccagcgct tcgccgaccc atttgcgcgc gaccacggga tccatcttca
     1081 taatggtcgg gcatggcgag aggatttcca ccagcgagaa gccgccacca ttcttctgga
     1141 tctccagcgc cttcttcacg gcacgcttgg ctttcatgat gttcttcatg tcgttcagcg
     1201 cgacccgctc aatgtaagtc ggggtctcca gcgcagccag aagctcgcac acgtgcagcg
     1261 gcaagccttc gttgctggcg cggcgtcccc agggcgaagt cgtggttacc tcgcccatca
     1321 gcgatgtcgg cgccatttgc ccgccagtca tgccgtagac cgaattgttg acaaagaata
     1381 cggaaatgtt ctcgccgcga ttggccgcgt gcacgatttc cgcggtgccg atggccgcca
     1441 ggtcgccgtc gccctggtac gccatcacga tggactcggg atgcgaacgc ttcagcgcgg
     1501 tcgccgccgc cggagcgcgt ccgtgggcta cctgcacatt gcccacgtcg aagtagtagt
     1561 aggcgaagac cgagcagccc accggactca ccaggatggt gcggtcctga agaccaagat
     1621 cggtgagcgc ttcggcgatc agcttatgga cgatgccgtg tccgcagccc ggacagtaat
     1681 gtgtctgctg ctgaagctcc cctttacgtt cgtagagatc gtagaatgct ggcgattttt
     1741 catggataac ttcgtagccg ttcacaattc ctcctagtac ccacccctac gccactgcgg
     1801 cgaagaatgg gggacctcgt cctacactaa ggccgacatg cgcgcaaaca cttgcgcagt
     1861 aatttcttcc gctgcgggga cattgccgcc cgtgcgccca tagaactcca ccggcaccgt
     1921 accgttgagc gtcagcttca cgtcttccag catcattccg gtgttcagct ccaccaccat
     1981 caccgacttc acgtgctgcg ctgctttacg
//
LOCUS       DATMGX010000725         3507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_4242,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000725 DATMGX010000000
VERSION     DATMGX010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4242"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VNR65_11955"
     CDS             <1..342
                     /locus_tag="VNR65_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19427.1"
                     /translation="FDQAAWNAHNYPALLDRYFTQSFRVPLYLVSGDNDKLGVAFETA
                     LLFKRVFERQPDLAELRVVDGDHSWKVWEATIDDAMRYVFQFADKPKLAPRTATATKG
                     LTSNQPESAHP"
     gene            complement(314..1153)
                     /locus_tag="VNR65_11960"
     CDS             complement(314..1153)
                     /locus_tag="VNR65_11960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ19428.1"
                     /translation="MDFAAFHEAALEADEIRHSLLLSIIGRLRTHPQSEIKTWTLGGP
                     GACAAQTPGYPIVLGNLSRAECHAFAEATSEADYPGVVGNGDMALWFAERACQLGVEF
                     VEKVPQEIQALWMPRQVPSAPGFPRQVSPKDFRLFRAWINAFIKEAVPSDPVPADEAL
                     MNDLRSRRHWLWTLEDSPVSMAAITRRTRTVAFVNSVYTPPEYRNRGFGAAITAIVSR
                     EIFEEGRRAACLYTDLRNPASNRCYAKLGFVPVCKSWLVIRSKEQARENPVSPTDAPI
                     PAD"
     gene            1257..2225
                     /locus_tag="VNR65_11965"
     CDS             1257..2225
                     /locus_tag="VNR65_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWJ19429.1"
                     /translation="MAIKDDNERQRKVLLDRREALTCMTWAGTGLLWALNGGVPLTKG
                     LIDVANAAPAHPEFTFVQISDSHIGFNKPANPDSQGTLREAIAKIKALPVKPAFMIHT
                     GDISHLSKDEEFDNADQLIKEAGLPVFYVPGEHDVIDDERGRSYLGRYGKNAQGAGWQ
                     SFDHNGVHYIGLVNVMDLKAGGLGNLGDEQLAWLEKDVAGLSSSTPIVVFAHIPLWTV
                     YPEWGWGTADAAKALSALKRFGSLTVLNGHIHQIMQKVEGNAVFHTARSTAFPQPAPG
                     TASSPGPMKVPAGELRNYLGIASVAFVVNSKPLAIIDTALSADPAL"
     gene            2240..2581
                     /locus_tag="VNR65_11970"
     CDS             2240..2581
                     /locus_tag="VNR65_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HWJ19430.1"
                     /translation="MKLNQSWILFLGSLAALTLTVCLGPIAKAEAASSVHIDNFTFTP
                     QEITITRGTTITWVNEDDIPHAIAANNKAFRSKAMDTEQNFSFTFSEPGSYEYFCSLH
                     PHMQGKVIVKE"
     gene            2636..3220
                     /locus_tag="VNR65_11975"
     CDS             2636..3220
                     /locus_tag="VNR65_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ19431.1"
                     /translation="MAKKPENASEFEAASLARRKAVFETAVLMHVNSAYTLARYLTRR
                     GDVADDIVQEALLRAYRSFDESRSSNVRAWLLTIVRNCFLTWKMRDHDKSAQRDFNDT
                     GTSCEQDGGAQEHETPESILIRREEDCTMRSLIEALPHPFREVLVLRDIEDMSYREIA
                     NITGVPMGTVMSRLARARKMFAADWKGLPMKEIL"
     gene            3220..>3507
                     /locus_tag="VNR65_11980"
     CDS             3220..>3507
                     /locus_tag="VNR65_11980"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWJ19432.1"
                     /translation="MNAVSRPCPERELLLHGLADGELDAANALAVEEHLGSCSACAAT
                     YSQIIRQKELFKNGGVRFNASPALRDRIRAAIAAEGEEEAVRAELLLKSLRG"
BASE COUNT          820 a          984 c          981 g          722 t
ORIGIN      
        1 ttcgatcagg cagcttggaa cgcccataat tatcctgctc tcctcgatcg ctacttcacg
       61 caatcattcc gagtaccgct ttaccttgtc tctggagata acgacaaact cggcgttgct
      121 ttcgagacag ctcttctttt caaacgcgtc tttgaacgtc agcccgacct ggccgagcta
      181 cgcgttgtcg atggcgatca cagttggaag gtctgggagg caacgatcga cgacgcgatg
      241 cggtacgttt ttcaattcgc cgacaagccg aagctggcgc cccgcacggc gaccgcgact
      301 aaagggctga cctctaatca gccggaatcg gcgcatccgt aggggaaact ggattttctc
      361 gcgcttgctc tttggaacga ataacgagcc agcttttgca gacaggaacg aaaccgagct
      421 ttgcgtagca tcggttagag gcggggttgc gaaggtcggt gtaaaggcag gcagctcggc
      481 ggccttcttc gaaaatctcg cgtgaaacga ttgccgtaat cgcagcaccg aagcctctat
      541 tacggtactc gggaggtgtg taaacagaat tgacgaaagc gacagtcctg gttcgacggg
      601 taattgcggc catggaaaca gggctgtcct caagagtcca aagccaatga cggcgggagc
      661 gcaaatcatt catcaaggcc tcgtcggcgg gaaccggatc ggaagggacg gcctccttta
      721 tgaaggcgtt gatccaggct cggaacagcc ggaagtcttt gggagatacc tgacggggga
      781 agccaggcgc gctcggtacc tggcggggca tccacagcgc ttggatttcc tggggaacct
      841 tttcaacgaa ttcgacgccg agctggcacg ctctctccgc gaaccaaagc gccatatcgc
      901 cgttaccgac aacgcccgga taatccgcct cgctcgtcgc ctccgcaaag gcgtgacact
      961 ccgccctcga cagattgcct agaacgatcg gatagcccgg cgtttgcgcc gcgcaagctc
     1021 cgggacctcc aagcgtccaa gtcttaatct cgctttgcgg gtgagttcgc aaacgcccga
     1081 taatgctcaa cagaaggcta tgcctgatct catcggcttc gagtgccgct tcatggaaag
     1141 ccgcgaaatc caaaggctgc atccgcaaac gactttctct gacaagaagc gatcgcgccg
     1201 ggaataaagc gcgctcgcga gaggtctacc ccataagttt aacagggaag gaccacatgg
     1261 caatcaagga tgataacgaa cggcagcgta aggtgctttt ggaccgccgc gaagctttaa
     1321 cgtgcatgac atgggccgga acgggcctcc tttgggcgtt gaacgggggg gtgcctctga
     1381 caaaggggct gatcgacgtc gcaaacgccg cgcccgcaca cccagaattt accttcgttc
     1441 agatcagcga cagtcatatc ggattcaaca aacccgctaa cccagactca caagggacgc
     1501 tgagggaggc tatcgcgaag atcaaagcgc tacctgtgaa gcctgcattc atgatccata
     1561 cgggggacat ttcgcatctg tccaaggatg aagaattcga caatgcggat cagctcatta
     1621 aagaggctgg cctgccggtg ttttacgtgc caggcgaaca cgacgtcata gacgacgagc
     1681 gcggccgatc ttacctcggg cggtacggaa agaatgccca aggcgcaggt tggcagagct
     1741 tcgatcataa cggcgtccac tatatcggtc ttgtgaacgt catggacctg aaagccggcg
     1801 gtttaggcaa tctcggcgac gagcaactcg cctggctgga aaaagacgtt gcaggccttt
     1861 cctccagcac gccaattgtc gtcttcgcgc atatcccgct ttggacggtc tatccggagt
     1921 ggggctgggg tacggctgat gcagcgaagg cgttatcggc gctgaaaagg ttcggttcgc
     1981 tgaccgtctt gaacggccac atccatcaaa tcatgcagaa agtcgaaggc aatgccgtct
     2041 tccacacggc ccgctcgaca gcgtttcctc aacccgcgcc agggaccgct tcgtcgcccg
     2101 gccccatgaa agtccctgcg ggcgagcttc gtaactatct cggcatcgcg agcgtagcct
     2161 ttgtcgtaaa cagcaagccg cttgcaatca tcgatacggc tctttcagcc gatccggcgc
     2221 tttgaaccga ggaaaagcta tgaagctgaa ccaatcttgg atccttttcc ttggatcgct
     2281 ggcagcgctg acgctcactg tatgtcttgg ccccattgcg aaagcggaag cggcgtcttc
     2341 cgtgcatatc gacaacttca cattcacacc gcaagagatc acgatcaccc gcggcacaac
     2401 catcacatgg gtcaacgaag atgatattcc tcatgccatt gctgcgaaca acaaagcgtt
     2461 ccggtcgaag gcaatggaca ccgagcagaa cttctctttc acgttcagcg aaccgggatc
     2521 atatgagtac ttctgttcgc ttcaccctca catgcaagga aaagtcatcg tgaaggagtg
     2581 actgcgatcc agagcctcat tgaggcggag aaaataaacg tgagcgatgc cgcaaatggc
     2641 aaaaaaacct gaaaacgcgt cagagtttga agcggcgtcg ctagcccgcc gaaaggcggt
     2701 ctttgaaaca gcggtgctca tgcacgtgaa cagcgcttat accctggctc gttatctcac
     2761 gcgccggggc gatgttgccg acgatattgt tcaggaagcc cttctgcgag cctaccgcag
     2821 tttcgatgaa tcccgcagca gtaacgtgcg ggcttggctt ctcacgatcg ttcgcaattg
     2881 tttcctgacc tggaaaatgc gcgaccacga caagtccgca caacgcgatt tcaacgacac
     2941 cggcacatca tgtgagcaag atggcggcgc ccaggaacat gaaactcctg agtctatttt
     3001 gatccgccgc gaagaggatt gcacaatgcg ctccctcatc gaagcgcttc cgcatccgtt
     3061 ccgtgaggtg ctcgttctca gggacattga ggatatgtct tatcgggaaa tagcaaacat
     3121 caccggcgtt ccgatgggca ccgtcatgtc tcgccttgcg cgagctcgta aaatgtttgc
     3181 tgcggactgg aagggcctgc cgatgaagga aattctgtga tgaatgccgt ttcccgtcct
     3241 tgccctgaac gcgagcttct tctccatggt ctcgccgatg gcgaacttga cgcggcaaat
     3301 gcgcttgcgg tggaggagca tttgggcagc tgcagtgcat gcgcagccac ctatagccaa
     3361 atcattcgac aaaaagagct attcaaaaat gggggcgtgc gtttcaatgc atctcctgcg
     3421 ctgcgagaca ggatacgagc ggcgattgcc gccgagggcg aagaggaagc agtgcgagct
     3481 gagcttcttc ttaagtcgtt gcgaggc
//
LOCUS       DATMGX010000726         2563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_419624, whole genome shotgun sequence.
ACCESSION   DATMGX010000726 DATMGX010000000
VERSION     DATMGX010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419624"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1286
                     /locus_tag="VNR65_11985"
     CDS             75..1286
                     /locus_tag="VNR65_11985"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWJ19433.1"
                     /translation="MRLLIDQAGLRSSLFAVATFSPVQILCFAAFLAAGGAIVYVLFG
                     YPLLLGWIAKRHNHPVKKDDQLRSVSIIIAVRNGEKFIRRKLESVLALNYPRELVEII
                     VVSDGSDDRTDEIAREFEAQRVRLLRVPRGGKAVALNAAVPCASGEILIFTDVRQQVD
                     PQALRNLIANFGDPAIGAVSGELAIVNDQTQEESDTSLYWKYELWLRKQMCRIDSSFG
                     CTGAFYGVRRSLFQPFPPDILLDDAYLPLTIFLKGYRVVYEPTAQMYDLPTALDSEFR
                     RKVRTQAGLYQFLRIMPELLSAKNRMCLHFLSGKFGRMIIPYCLILMAVATIGMSPGW
                     RMAAAIAQLGFYLLALLDYVLPASFPLKHLTSPIRTFVVLMTASLLGVKVLFVSSASL
                     WKETKVRHPAA"
     gene            1383..>2563
                     /locus_tag="VNR65_11990"
     CDS             1383..>2563
                     /locus_tag="VNR65_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HWJ19434.1"
                     /translation="MSGHDSGSPAFIFMQLRRQLLQVVLALALCAQALAGNGMHPVHA
                     PKAMVATLQPEASEAGVAIMRQGGNAVDAAVAVGFALAVVLPQAGNIGGGGFLLFRRA
                     DGEVHFLDFREKAPAKATANMYLDAQGNVVPGMSTLGYKSIGVPGSVAGLVFAQKHWG
                     KLTLKQVMEPAIRLARDGYVLGYDAARSLHDPNLAKFPESHRIFQRDGNFYQTGDVFK
                     QPALAKTLERIAENPDDFYHGAMARELVAAIEKGGGLITAEDLAQYEVKERQPIRGTY
                     RGYQIISAPPPSSGGVTLVESLNILEGYDLGKQGEGSAQNIHLTAEAYQRAFFDRAEF
                     LGDPDFSKVPVAQLLDKRYANAWRETIPLRRATPGNELRRPSVFSQLDSYASSHPQPK
                     A"
BASE COUNT          467 a          802 c          750 g          544 t
ORIGIN      
        1 acaatcgatt gtgactcgcg gagctgcacc ggggaacgaa gtccggcacc cacgagatct
       61 gcggctgcgc caacatgaga ctattgattg atcaggccgg gttgaggtcg agcttgttcg
      121 ccgtcgcgac gttctcacct gtccagattc tttgcttcgc cgcgtttctg gccgccggag
      181 gcgccatcgt ctacgtgctg ttcggctacc ccttgctgct gggatggata gcgaagaggc
      241 acaaccatcc ggtcaaaaag gacgaccagt tgcgttcggt gtccatcatc atcgccgtcc
      301 gcaatgggga gaagttcatt cgccgaaagc tggaatcggt gttggctctg aattaccccc
      361 gcgaactggt ggagatcatc gtcgtatccg acggttctga cgaccgcacc gacgagatcg
      421 cacgcgagtt cgaggcccag cgagtccgac tgctgcgagt tccgcgagga ggcaaggcgg
      481 tcgcgctcaa tgcagcagtg ccttgcgcct caggcgagat tctcattttc accgatgtcc
      541 gccagcaggt ggatcctcaa gcgcttcgga atctgatcgc gaacttcggc gatccggcga
      601 tcggcgccgt cagcggcgag cttgctatcg taaacgacca gacacaggaa gagtccgaca
      661 ctagtctcta ctggaagtac gaactttggc tgcgcaagca gatgtgccgc atcgactcca
      721 gcttcggctg taccggcgcg ttttatggag tgcggcgttc gctgttccag cccttcccgc
      781 cggacatcct ccttgacgat gcctatctgc cgctgaccat ctttcttaag ggctaccggg
      841 tggtttacga gccgacggcg cagatgtatg accttcccac cgctctggat tcggagtttc
      901 gtcgcaaggt gcgcacccag gccggcctat atcagttcct gcgcatcatg cccgagttgc
      961 tgagtgcgaa gaaccgcatg tgtttgcatt tcctctccgg caagttcgga cgcatgatca
     1021 ttccgtactg cctcatcctc atggccgtgg ctacaatcgg catgtcgccc ggttggagaa
     1081 tggccgcagc catcgcccag ttagggttct acctgcttgc actgctcgac tatgttttgc
     1141 ctgcaagttt ccccttaaag catctgactt cgcccatccg caccttcgtc gtgctgatga
     1201 ccgcgtcttt gctgggcgta aaggtcttgt tcgtgtcgtc ggcaagtttg tggaaagaaa
     1261 ccaaggttcg ccaccccgcg gcgtgattcc acgctcagga acatatttga ctcccctcgc
     1321 cgcgctgggc tagcctcaat gttttgtact tgcatggccg taacttcttg cgccgaacgc
     1381 atttgagcgg ccatgattcg ggttctcccg ccttcatctt tatgcaactg cgacgccaac
     1441 tgctgcaggt ggtacttgcg ctggctcttt gcgcccaggc tctcgccggc aatgggatgc
     1501 atccagtgca tgctcccaaa gccatggtgg ccacgctgca acccgaggca tccgaggcgg
     1561 gcgtcgcgat catgcgccag ggcggcaacg cagtggacgc cgcggttgcg gtcggcttcg
     1621 ccctcgccgt ggtgcttccc caggcaggca acatcggcgg cggcggcttc ctgctcttcc
     1681 gccgggccga cggtgaggtc cacttcctcg acttccgcga gaaagccccg gccaaagcca
     1741 ctgccaacat gtatctggat gcccagggca atgttgtccc aggcatgagc acgctgggct
     1801 acaaatcgat cggcgtcccc ggttcggtgg ccggcctggt attcgcgcag aagcattggg
     1861 gcaagctcac gctgaagcag gtgatggagc ccgcgatccg gctggcgcgt gatggctacg
     1921 tcctcggcta cgatgcggcg cgatcgctcc acgatcccaa cctggcgaag tttcccgaat
     1981 cgcatcgcat ctttcagcgc gacggcaact tctaccaaac gggcgacgtc ttcaagcagc
     2041 cagcgctggc gaagactctg gagcgcatcg ccgaaaatcc tgacgacttc taccatggcg
     2101 cgatggcgcg tgagctggtt gcggccatcg agaagggcgg cggcctgatc accgccgagg
     2161 accttgccca gtacgaggtg aaggagcggc agccaattcg tggcacctat cgagggtacc
     2221 agatcatcag cgctcccccg ccgtcttccg gaggggtcac cctggtcgag agcctcaaca
     2281 tcctcgaagg atatgaccta ggtaagcagg gtgaaggctc ggcgcagaac attcatctga
     2341 ccgccgaggc ttatcagcgc gcatttttcg accgcgccga gtttcttggg gaccctgatt
     2401 tctccaaggt cccggttgcg caactgctgg acaagcgata cgccaatgcc tggcgcgaaa
     2461 ccattccgct gcgccgcgcc acgcccggca acgagttgcg gcgtccttct gtcttctcgc
     2521 aactggacag ctatgccagt tcgcatccgc agccaaaggc gtt
//
LOCUS       DATMGX010000727         2572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_38360, whole genome shotgun sequence.
ACCESSION   DATMGX010000727 DATMGX010000000
VERSION     DATMGX010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38360"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VNR65_11995"
     CDS             <1..460
                     /locus_tag="VNR65_11995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWJ19435.1"
                     /translation="IVGGFAILWTGQYWIDSALSIGIGALILWSSFGIVRETLNILLE
                     GTPRGMSLEAVESAMRTIAGVSDVHDLHLWSIGSENHALSAHVKIADMATSESDTILR
                     EINEKLGQGFGIHHTTIQLEHVVCETAHACVIAVPEEGREHDHSGHDHAR"
     gene            608..1573
                     /locus_tag="VNR65_12000"
     CDS             608..1573
                     /locus_tag="VNR65_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HWJ19436.1"
                     /translation="MRFLAAVQLLPALESGEETLVGGQAVLEGVMMRSPHAWGIAVRK
                     PSGEIVTHSEPLERPSEQHKWMGWPVVRGVITLGHAMTLGFRALKFSANAALDEIPAN
                     ENGKKMEITGWMAGLNMVFSIAFFIFMYKYLPLLATTELKKVNPVFGQQFVFNLVDGL
                     IRIAMFLLFVWGVSLWKDIRRVYEYHGAEHKTVYAFENGDPLKTAAVQKYSTYHPRCG
                     TSFLMTVMIISMVVYMFIPVTTFWARFAERIILLPLIAGVSYEIIRFAAKHRGSLFAL
                     MTAPGLWLQRITTQPPDDSEVECAIVALDEAMKLEKQHGGELVIA"
     gene            1721..>2572
                     /gene="prfA"
                     /locus_tag="VNR65_12005"
     CDS             1721..>2572
                     /gene="prfA"
                     /locus_tag="VNR65_12005"
                     /inference="COORDINATES: protein motif:HMM:NF001859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWJ19437.1"
                     /translation="MFERLDQIEIRYEELTHALLSPDITNDSAKYQKTAKAHSEVAGI
                     VEKYREYKDLKRGIAESRAVLVDERDAEMRAYAEEELAKLEARIVGVEEDLKVLLLPK
                     DPNDEKNVVLEIRAGTGGDEATLFVAEVFRMYNRYAETQKWKVEVLSTSESGVGGLKE
                     VIALIEGDRVFSRLKYESGVHRVQRVPATEQQGRVHTSAITVAVLPEAEEVDVKIEAK
                     DLRIDTFCSSGPGGQSVNTTYSAVRITHIPTNTVVSCQDEKSQIKNREKGMRVLRSRL
                     YEGEMQKQ"
BASE COUNT          540 a          631 c          853 g          548 t
ORIGIN      
        1 aattgtgggg ggctttgcga tcctgtggac aggacaatac tggatcgact cggcgctgtc
       61 gatcgggatc ggcgcgctga ttttgtggtc gagcttcgga atcgtgcgcg agacgctgaa
      121 tattctgctc gagggcactc cgcgcggcat gagcctggag gcggtggaat cggcgatgcg
      181 gacgattgcg ggcgtgagcg atgtgcatga tctgcatctg tggagcattg ggtcggaaaa
      241 ccacgcgctc tcggcgcatg taaagatagc ggacatggcg acgtccgaga gcgacacgat
      301 tctgcgcgag atcaacgaga aactcgggca gggatttggg attcaccata cgacgatcca
      361 gttggaacat gtggtgtgcg agacggcgca tgcgtgcgtg atcgcggtgc cggaggaggg
      421 gcgtgagcac gaccattccg ggcacgacca cgcccgctga atgccggggc agcacctgta
      481 tcttcttgat gagcaattgg ttacgtgatt gtgccacgag cggcaaccgg cttctgtggt
      541 atcctgatag ttggagtcct tcctccctga gtatcatcca atggctatgc gacgttggcg
      601 gcaaattttg cgttttttgg cggcggtaca gctgctcccg gctctggaga gcggcgagga
      661 aaccctcgtc ggcgggcaag cggtgctgga gggcgtgatg atgcgttcgc cccatgcctg
      721 gggaattgcc gtgcgcaagc cttcgggcga gattgttacc cacagcgagc cgctggagcg
      781 gccttccgag cagcataaat ggatgggctg gccggtggtg cgtggggtga taacgctggg
      841 acacgcgatg acgctggggt tccgcgctct gaaattttcc gcgaatgcgg cgctggatga
      901 gatcccggcc aacgagaatg gcaagaagat ggaaatcacg ggctggatgg cgggattgaa
      961 tatggttttc tccatcgcct ttttcatctt catgtacaag tacctgccgc tgctcgctac
     1021 tacggaactg aaaaaggtga atccggtttt cgggcagcag tttgtgttca atctcgtcga
     1081 cgggctgatc cggatcgcga tgttcctgct gtttgtgtgg ggcgtttcgc tgtggaagga
     1141 cattcggcgt gtgtacgagt atcacggcgc cgaacacaag acggtgtatg cgttcgagaa
     1201 tggcgatccg ctcaaaaccg cggccgtgca gaagtactcg acgtatcatc cgcggtgcgg
     1261 aacgagcttt ctgatgacgg tgatgatcat ctcgatggtc gtgtacatgt tcatcccggt
     1321 gactacgttc tgggcgcggt ttgccgaacg cattattttg ctcccgctga ttgcgggtgt
     1381 ttcctatgaa attattcgct ttgccgccaa gcatcgcggc tcgctgttcg cgctgatgac
     1441 ggcgccggga ctttggctgc agcggattac gacccagcct ccggatgata gcgaagttga
     1501 gtgcgcgatt gtcgctctgg atgaagcgat gaagctggaa aagcagcacg gcggcgaact
     1561 cgtcattgcc tagcggtgcc caccgcgcat tgttcccagt tctcggttct cagtaaaacc
     1621 ccttacaata gaattcacgg gaaccggcac gggactgatt ctgcgcggcc atcatggcgt
     1681 gtgattgttt tgagacctaa gaaccgagaa ctgagaactc atgtttgaac gtcttgacca
     1741 aatcgaaatc cgctacgagg aactgacgca cgcccttttg tcgccggata ttaccaatga
     1801 ttcggcgaaa taccagaaga ctgccaaggc gcatagcgaa gtcgcgggga tcgttgagaa
     1861 gtatcgcgag tacaaagacc tgaagcgcgg catcgccgag agccgggccg tgctggtgga
     1921 tgagagggat gcagagatgc gcgcctacgc ggaagaggaa ctggcgaagc tcgaagcgcg
     1981 catcgttggc gtcgaagaag acctcaaggt tctgctgctg ccgaaggatc ctaacgacga
     2041 gaagaacgtg gttctggaaa ttcgtgccgg gacgggtggc gatgaggcta cgttattcgt
     2101 agcggaggtc ttccgcatgt acaaccgcta tgccgagacg cagaagtgga aggtggaagt
     2161 gctctccacg tcggagtcgg gcgtgggcgg gttgaaggaa gtcatcgcgc tgatcgaagg
     2221 cgaccgggtc ttttcgcgcc tgaagtatga aagtggtgtg catcgtgtgc aacgcgtgcc
     2281 tgcgaccgaa cagcaagggc gggtacatac gtcggcgatc acagtcgcgg tgctaccgga
     2341 agcggaagaa gtggacgtca agattgaagc taaagacctg cgcatcgata cgttctgttc
     2401 gtcggggccg ggcgggcagt cggtcaatac gacgtattcg gcggtgcgga ttacgcacat
     2461 tcctacgaac acggtggtga gctgtcagga tgagaagtct cagatcaaga accgcgaaaa
     2521 aggtatgcgc gtgctgcgct cgcggctcta cgaaggcgag atgcagaagc ag
//
LOCUS       DATMGX010000728         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_430707, whole genome shotgun sequence.
ACCESSION   DATMGX010000728 DATMGX010000000
VERSION     DATMGX010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430707"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VNR65_12010"
     CDS             complement(<1..598)
                     /locus_tag="VNR65_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWJ19438.1"
                     /translation="MPSFSNSLEESLHRAIEFANERHHEYATLEHLLLSLIDDRDASS
                     VMRACNVDIERLRAKLTDFLDKELTNLILDQDSEAQPTASFQRVIHRAVVHVQSSGRE
                     EVTGANVLVAVFAERESNAAYFLQEQDMTRFDAVNFISHGISKRAGMSEPRGVRGVDE
                     DAATVEGADDKKRSTDALEAYCVNLNQKAKDGRIDPLIG"
     gene            complement(606..935)
                     /gene="clpS"
                     /locus_tag="VNR65_12015"
     CDS             complement(606..935)
                     /gene="clpS"
                     /locus_tag="VNR65_12015"
                     /inference="COORDINATES: protein motif:HMM:NF000672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWJ19439.1"
                     /translation="MAGNNGFKNGDDLKTGVVTKTRPKTKRPSLYKVLLLNDDYTPME
                     FVIYVLERFFNKQREEATRIMLHVHQKGVGVCGVYTYEVAETKVTQVMNFSRQHQHPL
                     QCTMEKE"
     gene            1424..>2045
                     /locus_tag="VNR65_12020"
     CDS             1424..>2045
                     /locus_tag="VNR65_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWJ19440.1"
                     /translation="MCVLTALLTLASNAEARRHHVRQYSSAPPAAMAVDLYTGRVLYS
                     KNADIQRYPASITKVMTLYLLFDALRDGKLTMRTQLRVSEHAADQSPTKLDLSAGDTI
                     SVADAIGAIVTKSANDMAVTVAEALAGSEDEFARRMTQKARTIGMLNTTFRNASGLPN
                     SEQRTTAQDLITLGKNVLADHPERSKVFSTKYFQYKGEIFRNHNTML"
BASE COUNT          435 a          590 c          557 g          463 t
ORIGIN      
        1 ggccgatcag cggatcaatg cggccatctt tcgctttctg gttgagattt acgcaataag
       61 cttcaagcgc gtctgtgctg cgcttcttgt cgtccgcacc ttccactgtc gctgcgtcct
      121 catcaacgcc gcgcacgcca cggggctcgg acatccccgc gcgtttggag atgccgtgcg
      181 agatgaagtt gaccgcgtcg aaacgggtca tatcctgctc ttgcaggaaa taggccgcgt
      241 tgctctcccg ctccgcgaag acggcgacga gaacattggc gccggtgacc tcttcccggc
      301 cggacgactg gacatgaaca acggcgcgat ggatgacacg ctggaagctg gctgtgggct
      361 gggcttcgct gtcctgatcg aggatcaggt tggtcagctc cttgtcgaga aaatcggtca
      421 gcttggctcg caggcgctcg atatcgacat tacaggccct catcacggat gaggcgtcgc
      481 gatcatcgat aagtgagaga aggagatgtt cgagggttgc gtactcgtga tgacgctcgt
      541 tggcgaattc gattgcgcgg tggagcgact cttccaggct gttcgaaaat gatggcactc
      601 cgcacctact ccttttccat cgtacactgc aagggatgct gatgctgacg cgaaaagttc
      661 atcacctgtg taactttcgt ctcagcaact tcgtaggtat aaacgccgca tactccgacg
      721 cccttctgat gcacatgaag cattatgcgc gtcgcctcct ccctctgttt attgaagaac
      781 cgctcaagca cataaatgac gaactccatt ggagtgtaat cgtcgttcaa aaggagaact
      841 ttgtacaggc tgggacgctt cgtctttgga cgcgtttttg tgacgacgcc cgttttcagg
      901 tcatcgccat tcttgaagcc gttattgcct gccatcgccg aaaccatcga gaaccaatca
      961 aaagccttaa aaagccttag catatcgggc gtttctcatt caccgcaggg gcaccccatc
     1021 aaggtagtca tcaccttaca tactcccgtt tatcggtttc ctctgtcaag gcccgcaagt
     1081 gtaaatgctt ggccgaatat atggttcgct tcgcgccttg gaacaggtct gccagcctcg
     1141 cggtgaccaa actttagaaa gtctcggtta ctttttgata gggaccagcc ggtcgctttg
     1201 cctgacgggg atgggtaaga aagtattaag gccctccctg gtatacaagt gtcgccgaac
     1261 aaccttgcgt agaggacatt ccggcggtac gcgctcgcct gccttgctca cgacaaagtt
     1321 tttgggtttt cacgctgggt agcgtttgga aaagtcgatg tcagtaccaa agcgggagcg
     1381 gggctcggct ccaagagttc ggtcaaggcc gcgagccctc atagtgtgcg tgttaaccgc
     1441 gttgctgacg ttggcttcga atgccgaagc gcgccgccat catgtgcggc aatactcgtc
     1501 agctcctccg gccgcgatgg cggtcgacct ctacacggga cgtgttctct actccaagaa
     1561 tgccgatatc cagcggtacc cggcctcgat cacgaaagtg atgacgcttt acctgctgtt
     1621 cgatgccctg cgcgacggga agctgacgat gagaacgcag ctccgcgtct cagagcacgc
     1681 ggccgaccaa tctccgacaa agcttgacct ttcggccggc gatacgattt ccgtcgctga
     1741 tgccattggt gccatcgtga cgaagtcagc gaacgacatg gccgtgaccg tcgccgaggc
     1801 tcttgccggt tccgaagacg aattcgcgcg gcggatgacg cagaaggccc gcacgatagg
     1861 aatgctgaac accacgttcc gcaacgcgtc aggccttcct aattccgagc agagaaccac
     1921 ggctcaggac ctgatcaccc tcgggaaaaa cgtcctcgcc gaccaccccg agcgcagcaa
     1981 ggtcttctcc accaagtatt ttcaatacaa gggcgagatc tttcggaatc ataacaccat
     2041 gctct
//
LOCUS       DATMGX010000729         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_288317, whole genome shotgun sequence.
ACCESSION   DATMGX010000729 DATMGX010000000
VERSION     DATMGX010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288317"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /gene="ubiG"
                     /locus_tag="VNR65_12025"
     CDS             complement(<1..329)
                     /gene="ubiG"
                     /locus_tag="VNR65_12025"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: protein motif:HMM:TIGR01983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HWJ19441.1"
                     /translation="MGIEAETRHSPSLDMAELRHFEGLAKEWWDERGKFGGLHAFNPA
                     RLAFIVSEVQSWRDQQNQSFRPLDGLSVLDIGCGGGILSEPLARLGGSVTGIDPVEES
                     IGVATLHA"
     gene            427..1671
                     /locus_tag="VNR65_12030"
     CDS             427..1671
                     /locus_tag="VNR65_12030"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWJ19442.1"
                     /translation="MKFGGTSVADIGRIRNSALHVKREAEAGNRVVVVVSAMAGHTNQ
                     LVAWTREAHAFHDTREYDAVVSSGEQITAGLMAIILQSMGVTARSWQGWQIPLKTDDA
                     HAVARIDRIESVELKRRLDEGEVAVVAGFQGLSPRGRITTLGRGGSDTTAVALAAALK
                     ADRCDIYTDVDGVYTTDPRIVPSARRLPRVSYEEMLEMASLGAKVLQTRSVELAMAQG
                     VRVQVRSSFDPPETCEPARAGIPEKCGTLVCDETEMLEQHVVSGIAYAKDEAKVTVLS
                     IEDKPGVAASIFMPLAEANINVDMIVQNISEDGRKTDLTFTVAASDLQRALQVLRDRK
                     DMIRYGDLRGDTDMVKVSVIGVGMRSHAGVAAEMFQALADKEINIQAISTSEIKISVL
                     LGAAHTEAAVKTLHSLFGLDRT"
     gene            1756..>2212
                     /locus_tag="VNR65_12035"
     CDS             1756..>2212
                     /locus_tag="VNR65_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWJ19443.1"
                     /translation="MPWTERAPWVLIRQLRAIMASASEPQAKLDSIVKVIASNMIAEV
                     SSIYVRLPDDALELFATEGLRKEAVHALRMKKGEGLVGLISAWGEPVNLSDAQKHPAF
                     SYKPETGEEIYHSFLGVPIVRERRTIGVLTVQNKIERKYTEEEVEALETT"
BASE COUNT          495 a          624 c          662 g          431 t
ORIGIN      
        1 gcgtggagcg tcgcgacgcc gatggactcc tcgaccgggt cgattcctgt tacacttccg
       61 ccgagccgcg ccagcggttc cgacaggatg ccgcctccgc agccgatgtc gagcacagac
      121 aggccgtcca gggggcgaaa ggattggttt tgctgatcac gccaactctg gacttcagag
      181 acaataaagg cgagccgcgc aggattgaag gcatgcagac caccaaattt cccgcgctcg
      241 tcccaccact ccttggcaag cccttcaaaa tgacgcaact ctgccatgtc aagcgacggg
      301 ctatgccgtg tttctgcctc aatccccatt gatggcgttc cttatgtccc tgggcgctca
      361 gcgttttcgc cgccgccgct ttttaatttc actcttgcac catagctcac gatcatgctc
      421 ctcgtcatga agtttggcgg aacctccgtc gctgacatag gccgtataag aaattcggcg
      481 ctccacgtga agagggaggc cgaggctggc aatcgcgtcg ttgtcgttgt ctcggcgatg
      541 gccggacata caaaccagct cgtcgcttgg actcgcgagg cgcatgcctt ccacgacacg
      601 cgggagtatg acgccgtggt gtcctcaggg gagcagatca cggccgggtt aatggccatc
      661 attctgcaat ccatgggcgt aaccgcccgc tcctggcagg gatggcagat cccactcaaa
      721 acggacgacg cgcacgccgt tgcgcggatc gaccgtatag aatcggtcga gctgaagcgc
      781 cgccttgatg agggagaggt ggccgtcgtg gcggggtttc aagggctcag ccccaggggg
      841 cggataacta ctctcggccg cggcggctcc gacacgaccg ctgtagccct tgccgccgcc
      901 ctgaaagcgg accgttgcga tatctatacg gatgtggatg gtgtgtacac aactgatcca
      961 cgtattgtgc cctcggctag gcgattacct agggtgtcgt atgaggaaat gctcgaaatg
     1021 gcgtctctgg gcgcaaaagt gctccagact cgttccgtag agcttgcaat ggctcaaggg
     1081 gtgcgtgtac aagtccgttc tagcttcgat ccaccagaaa cctgcgaacc cgctcgagcc
     1141 ggaattccgg aaaagtgcgg gacgctcgtt tgtgatgaaa cggaaatgtt agagcaacac
     1201 gttgttagcg gcatagctta cgccaaagat gaagcgaagg tgaccgtcct cagcatcgag
     1261 gacaagcccg gcgttgcagc gagcattttc atgccgctgg ccgaggcaaa catcaacgta
     1321 gacatgatcg tccagaatat ctcggaggac ggccgcaaga cggatctcac gtttactgtc
     1381 gcggcatccg atctgcagcg cgcgctacag gtgctgcgcg atcgcaagga tatgatcagg
     1441 tatggggatt tacgcgggga taccgatatg gtgaaggtat cggttatcgg cgttggtatg
     1501 cggagccatg ccggcgtagc ggcggagatg ttccaggcct tggccgataa agagatcaac
     1561 atccaggcca tatcgacgtc agaaatcaag attagcgtct tgctaggcgc cgcccatacg
     1621 gaggctgccg tcaagacact ccatagcctt ttcggcttag atcgaacgta aagaaacagg
     1681 caattcgaag aagaggcgcg acaaacggaa aaggcgccac acattcacaa cccaaaggtc
     1741 attggaacca ggcaaatgcc ctggactgag cgtgcaccat gggtgctgat ccggcaattg
     1801 cgggcgatca tggcgagtgc gtcagagccc caggcgaagc tggacagcat tgtcaaagtg
     1861 atagcctcga acatgattgc cgaggtgagc tcgatctatg tccggctgcc agacgatgcc
     1921 cttgagctct tcgcgacgga aggccttaga aaagaggccg tccatgcgct gcgcatgaag
     1981 aaaggcgagg gtcttgtcgg actcatctcc gcctggggcg agccggtcaa cctctcggac
     2041 gcccagaagc accctgcctt ctcatacaag cctgaaaccg gggaggagat ttatcactct
     2101 ttcctcggcg tgccgatcgt ccgtgagcgg cgcaccatcg gtgttctcac cgttcagaac
     2161 aagatagaac ggaaatacac ggaggaggag gtcgaggcgc tcgaaacgac ag
//
LOCUS       DATMGX010000730         2418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_282756, whole genome shotgun sequence.
ACCESSION   DATMGX010000730 DATMGX010000000
VERSION     DATMGX010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282756"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /gene="rplP"
                     /locus_tag="VNR65_12040"
     CDS             <1..410
                     /gene="rplP"
                     /locus_tag="VNR65_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWJ19444.1"
                     /translation="FRKAFKGRMNGNAKGGTSLNFGSFGLKALEPERVTARQIEAARR
                     AITRQMKRAGRVWIRIFPDLPVSKKPTEVRMGKGKGSPEFWAARVKPGKIMFEIDGVG
                     EETAREALRLGAAKLPIRTRVVTRLGELATCKE"
     gene            418..621
                     /gene="rpmC"
                     /locus_tag="VNR65_12045"
     CDS             418..621
                     /gene="rpmC"
                     /locus_tag="VNR65_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWJ19445.1"
                     /translation="MKIEDIRNFTLDQLEDELIKLKREQFNLRFQKATSQLTNTARVR
                     EVRRTIARIKTAERDKRVAEAQS"
     gene            633..884
                     /gene="rpsQ"
                     /locus_tag="VNR65_12050"
     CDS             633..884
                     /gene="rpsQ"
                     /locus_tag="VNR65_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWJ19446.1"
                     /translation="MPKRVLQGVVVSDKNDKTVVVQVERRLTHPILKKTVRLTKKYHA
                     HDEANAFHEGDVVRIQECPPVSKNKRWVVLDQVSARASE"
     gene            930..1298
                     /gene="rplN"
                     /locus_tag="VNR65_12055"
     CDS             930..1298
                     /gene="rplN"
                     /locus_tag="VNR65_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWJ19447.1"
                     /translation="MIQMQTNLDVADNSGARRVMCIKVLGGSMRRTATIGDVIVVSVK
                     EAIPKGRVKKGQVMKAVIVRTSFPVRRPDGSVIRFDSNAAVLINNQREPIGTRIFGPV
                     PRELRAKQHMKIISLAPEVL"
     gene            1298..1624
                     /gene="rplX"
                     /locus_tag="VNR65_12060"
     CDS             1298..1624
                     /gene="rplX"
                     /locus_tag="VNR65_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWJ19448.1"
                     /translation="MTQPKLKIKKGDRIVVLAGRDKGKRGEVLQVMPLENRALVQGVN
                     VVRRHQKQSAQQEGGIVSKEASIHLSNIAIEDPADGAPTRIGYKFMDDGRKVRFAKRS
                     GEAIDD"
     gene            1650..2189
                     /gene="rplE"
                     /locus_tag="VNR65_12065"
     CDS             1650..2189
                     /gene="rplE"
                     /locus_tag="VNR65_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWJ19449.1"
                     /translation="MPRLKEQYLEVVRPALLAEFKYTNPMRVPHIEKVVLNMGIGEAT
                     ADRKKVEQAARDMALIAGQKPVMMKARQSIAGFKLRDNMAIGCKVTLRKARMYEFLDR
                     LVTIALPRVKDFRGLNPKSFDGRGNYALGIKEHIIFPEIDYDKVEQIWGMDIIVCTSA
                     KTDDEARALLKQINFPFRQ"
     gene            2210..>2418
                     /gene="rpsN"
                     /locus_tag="VNR65_12070"
     CDS             2210..>2418
                     /gene="rpsN"
                     /locus_tag="VNR65_12070"
                     /inference="COORDINATES: protein motif:HMM:NF006477.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HWJ19450.1"
                     /translation="MAKTGMIERNNKRRRLCGRDAEKRAALKEIANNKELPMEECFGA
                     RLKLARLPRNGSPTRIRNRCEVTGRP"
BASE COUNT          592 a          615 c          745 g          466 t
ORIGIN      
        1 aattcaggaa ggccttcaag ggccggatga atggtaacgc caaaggcggc acttcgctta
       61 actttggcag tttcggcctc aaggctctcg aaccagagcg cgtgactgcg cgtcagatcg
      121 aggctgcgcg gcgggcgatc acgcgtcaga tgaagcgcgc cgggcgcgtt tggatacgga
      181 ttttcccgga cttgcccgta tccaaaaagc ctactgaggt tcgcatgggt aagggcaagg
      241 gatcaccgga attttgggct gctcgcgtga agccgggcaa gatcatgttc gagatcgacg
      301 gtgtgggaga ggagactgcg cgggaggcgc ttcggcttgg cgccgcgaag cttcctattc
      361 gcacacgcgt cgtgacccgc cttggcgaat tagcgacgtg caaggagtag ggcggccatg
      421 aaaatcgaag atattcgtaa ttttacgctc gaccaactcg aagacgagct cattaagctg
      481 aagcgcgaac aattcaattt gcgattccag aaggcgactt cgcaacttac caatacggcg
      541 cgtgtccgtg aagtccgtcg cacgattgcg cggatcaaga ccgccgaacg tgataagcgc
      601 gtcgccgaag cgcaaagtta aggaaaacgg aaatgccaaa gcgcgttttg cagggtgtgg
      661 tcgtctcgga caagaacgat aagaccgttg tcgtccaggt agagcggcgt ctgacgcacc
      721 cgatcctaaa aaagaccgtg cgtcttacga agaagtacca tgcccacgat gaggcgaatg
      781 cgttccacga gggcgacgtc gtacgtatcc aggaatgtcc tccggtttca aagaataagc
      841 ggtgggtggt tttggatcag gtgtctgccc gggcgtccga gtgaaataaa cgtgaggtgg
      901 gcttaagctc gcccgaaagg tgaactgcaa tgatacagat gcaaactaat ctggatgtcg
      961 ccgacaattc gggcgcacgc cgggtcatgt gcatcaaggt cctcggaggc tccatgcggc
     1021 ggaccgccac aatcggcgac gttatcgtcg tcagtgtgaa ggaggccatc ccgaaggggc
     1081 gtgtaaagaa aggccaggtg atgaaggcgg ttattgtccg tacctcgttt ccagtccgcc
     1141 gtccggacgg tagcgtgata cgcttcgaca gcaacgccgc tgttctcatc aacaatcagc
     1201 gtgaaccgat cgggacacgc atctttggcc cagtgccccg cgaactccgc gccaagcagc
     1261 atatgaagat aatttcgctt gctcccgagg ttctctgatg acgcagccga agcttaagat
     1321 aaaaaagggc gatcgtatcg tggtgcttgc gggccgggat aagggcaagc gtggcgaagt
     1381 cctgcaggtg atgccgttgg aaaatcgggc gctggtccag ggcgtaaacg tcgtccgtcg
     1441 gcaccagaag caatcagcgc aacaagaagg cgggattgtt tccaaagagg catctattca
     1501 tctctcgaat atcgcaatag aagatccggc agacggggca ccgacccgca tcggatataa
     1561 attcatggat gatgggcgga aggtgcggtt cgccaagcgt tcaggcgagg cgatcgatga
     1621 ctaagacagt gaataattca gccggctacg tgcctcgcct gaaggaacag taccttgaag
     1681 tcgtgcgtcc tgcgttgctt gccgaattca agtatacaaa tccaatgcga gttccgcata
     1741 tcgaaaaagt cgtgctcaac atgggtatcg gcgaggctac cgcggaccgt aaaaaggtgg
     1801 agcaggccgc acgtgatatg gcgttgatcg ccggtcagaa gcccgtgatg atgaaagccc
     1861 ggcaatcgat cgctggcttt aagctccgcg ataacatggc gatcggctgc aaggttacgc
     1921 ttcggaaagc gcgcatgtac gagtttctgg atcggctcgt aacaattgcg ctgccgcgcg
     1981 tgaaggattt tcgcggcctc aatccaaaaa gtttcgacgg ccgtggcaac tatgctctcg
     2041 gcattaagga acacatcatc tttccagaga tcgactacga taaggtcgag cagatctggg
     2101 gcatggacat catcgtgtgc acttcggcca aaacagatga tgaggctcgt gcgcttttga
     2161 aacaaatcaa cttccccttc cggcaatagg gcgaggcgga ggagagacta tggccaaaac
     2221 tggcatgatc gagcggaaca acaagcggcg gcggctgtgc gggagagacg ctgagaaacg
     2281 cgcggcgctg aaggaaatcg cgaataacaa agagctaccg atggaagagt gcttcggggc
     2341 tcgcctgaag ttggcgcgac tgccacgaaa cggatcgcca acgcgcattc gcaatcgctg
     2401 cgaggtcacg gggcgccc
//
LOCUS       DATMGX010000731         2686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_141910, whole genome shotgun sequence.
ACCESSION   DATMGX010000731 DATMGX010000000
VERSION     DATMGX010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141910"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VNR65_12075"
     CDS             complement(<1..1537)
                     /locus_tag="VNR65_12075"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWJ19451.1"
                     /translation="MNAATGARYTAASSATGAFDFELLAAGDYSARVTAQGMAPQVTP
                     QLHVDIGGTALLEFHLIVAGAQEKVTVSGAPALVEPQTSTVSTLLDERAVNDFPLNGR
                     RFSDLALFSPGVTQDPRSLTSSSNGDLSFGGIRGFQNTTLVDGADYNNAFFSQARGRY
                     RAPYQFSTEVVQEFRVSTNSYGAEQGRSGGAVVNVVTKSGGNHVHGTAFYYLRDSSFG
                     ASNPFLAFKPHNRQQQVGGTIGGPIKRNKIFFFAGFDQHIFHVPNVVEFLDGSSKVVP
                     AAETGPFTPGDYEATDQALVFAAASRLTSLAGEYPAGQIGNTAYGKLDLNLSSRQQLS
                     LRLNTTRYWGSNNVFLDPASPLTYDANNNNGEEVVSTETASLSLTSGLSQRWISHFRA
                     QFSRDRQQSYSNTSDVLVKIPTILDGMGRSNLLPRQTREHRLHLAETMSHEGSRNTWK
                     FGGDALLTWIYDFFPSQQSGEYLFYPVKVNPFTFEPMEARLQLSPLRAYAHQVPHYCL
                     QNFG"
     gene            2185..2646
                     /locus_tag="VNR65_12080"
     CDS             2185..2646
                     /locus_tag="VNR65_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWJ19452.1"
                     /translation="MDESAQIIGDVVLGEHASVWMNAVVRGDVHEIRIGAYSNVQDNS
                     VLHGMKQQWGVYLGEYVTVGHSVTLHGCVIEDRCLIGMGSIILNGARIGAGSIIAAGT
                     LIPEKTIVEPGSLWMGSPGKFRRMLGPGEDETIMRYARNYLGYKDEYLRER"
BASE COUNT          554 a          728 c          884 g          520 t
ORIGIN      
        1 tgccaaagtt ctgcagacaa taatgcggca cttgatgggc gtaagcacgc agagggctga
       61 gttggagcct ggcttccatg ggctcgaagg tgaacggatt gaccttgacg ggatagaaaa
      121 gatattcgcc gctctgctgg ctggggaaga agtcgtaaat ccaagtcagc agcgcgtcgc
      181 cgccgaactt ccacgtgttc cgcgaaccct cgtgactcat cgtttctgcg agatgcaagc
      241 gatgctcgcg ggtctggcgg ggcagaagat tcgagcgtcc catgccgtcg aggatggtag
      301 ggatcttgac cagtacatcg ctggtattgc tataggactg ctggcggtcc cgcgagaact
      361 gcgcgcgaaa gtggctgatc cagcgctgcg aaaggccgga cgtcagcgac aaagacgcag
      421 tctccgtgga gacaacttcc tcgccgttat tgttgttggc atcgtaggtg agtgggctgg
      481 cgggatcgag gaagacgttg ttcgatcccc aatagcgcgt ggtgttgagg cgcaacgaca
      541 gttgctggcg cgaagagaga ttgaggtcga gtttgccgta agctgtgttg ccgatttgcc
      601 ccgcgggata ttctcccgcc agcgaggtca ggcgtgacgc cgcagcgaag accagggctt
      661 gatcggtcgc ttcgtagtct cccggagtaa aaggtccggt ttcggccgcg ggcacgactt
      721 ttgaactgcc atcaaggaat tcgacgacgt tgggcacgtg gaagatgtgc tggtcgaatc
      781 cggcaaaaaa gaaaatcttg ttgcgcttga tgggcccgcc gatggtgccc ccgacctgct
      841 gctggcggtt gtgcggcttg aacgccagaa agggattcga tgcaccgaaa gaactgtcgc
      901 gcagatagta aaacgcggtg ccgtgcacat gattcccccc ggacttggta acgacgttga
      961 cgacagctcc gcccgagcgt ccctgctctg cgccgtagga attcgtcgac acccggaact
     1021 cctgcacgac ttccgtggag aactggtagg gcgcgcggta gcgtccgcgg gcctgcgaga
     1081 aaaacgcgtt gttgtagtcg gcgccgtcga ccagggtggt gttctgaaat ccgcgaatgc
     1141 cgccgaagga gagatcgccg ttgctggagg aagtgaggct gcgcggatcc tgcgtgactc
     1201 cgggcgagaa cagcgccaag tccgagaacc gccggccgtt cagtgggaaa tcgttcaccg
     1261 cgcgttcgtc gagcagcgtg gagacggtac tggtctgcgg ttcgaccaac gcaggcgcgc
     1321 ccgaaaccgt taccttctct tgtgctccgg cgacgatcag atggaattca aggagcgcgg
     1381 tcccgccgat atcgacgtgc agttggggag tgacttgagg agccattcct tgcgccgtga
     1441 cgcgcgcgga gtagtcgccg gcagcgagca actcaaagtc gaacgctcca gtggcggaac
     1501 tggctgcagt gtagcgtgcc ccggtcgcgg cgttcaccag aacgatcgaa gcttgcgcga
     1561 tgactccgcc ggtggaatcg agcacggtgc cgcggaccgc tccgctcgcg tcctgtgccg
     1621 ccagcagcgt tggcatcatg atcagaaaaa agatgaaggc cggaagaaaa accattcgcg
     1681 tcgcagacat gagtcgcctc gaagttcggg atcgaactcg gagggacccg acgcgcgtta
     1741 ccggacgcga cgcgaggggc ccgcggagaa gtgccggagg gagcatccta gtcgggctcg
     1801 gccggaaact tctccgcggg tatggaaaac caagatttat atagcacgcg cagggcgccc
     1861 agttctgtga ttgaacgagg cttttcggga tgagttcccg gagtgggaat ggaagacaac
     1921 taaccacaga ggacacagag gacacggagg gaaccgggcg aatggtgatc gcattctctt
     1981 tggcggtgcg aaacgaaaaa cccagtcgag ctttgcccga cccttcggct gcgctcaggg
     2041 caggctagcc gcgggcggct gtccccacat gagttcttgc ggcccttacg ctggaatgcg
     2101 ttctcgtggt attttcaatt tctttctcga actatgattc gaccttataa aggtgtgcga
     2161 ccgacgattc ctgcgacgtg tttcgtggac gaatcggcgc agattatcgg cgacgtcgtg
     2221 ctgggcgagc acgcttcggt gtggatgaac gccgtggtgc gcggcgacgt ccacgagatc
     2281 cgcattggcg cgtactccaa cgtgcaggac aacagcgtcc tccacggtat gaaacagcag
     2341 tggggcgtct acctcggcga gtacgtgacg gttgggcatt cggttacgct gcacggctgc
     2401 gtgatcgaag accgctgcct gatcggcatg gggtcgatca ttttgaatgg cgcccgcatc
     2461 ggcgcaggtt caatcatcgc tgccggaacg ttgattcctg agaaaacaat cgtcgagcca
     2521 ggatccctgt ggatgggctc cccaggaaag ttccgccgaa tgttaggccc gggcgaggac
     2581 gagacgatca tgcgctacgc gcgcaattat ctcggctata aggatgagta tctgcgagag
     2641 agatgattcg cgagtccccg gtcctaaccc acgagcgccg gcgact
//
LOCUS       DATMGX010000732         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_460500, whole genome shotgun sequence.
ACCESSION   DATMGX010000732 DATMGX010000000
VERSION     DATMGX010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460500"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VNR65_12085"
     CDS             complement(<1..1136)
                     /locus_tag="VNR65_12085"
                     /inference="COORDINATES: protein motif:HMM:NF023151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWJ19453.1"
                     /translation="MSEPLVDPAVLEKVRGWRPYVEAKFGFRNHWYPAMFSYELLEDH
                     PVEAKLLGESILLNRLDGRPLAIRNRCLHRGVKFSKQIECYKKGTITCWYHAWTYNWD
                     DGKLSDIITNPNSSLIGKHQLKTYPTQEAKGLIFVFVGDIDPPDLASDVPPGFLDEGM
                     AIHGKRRMVKSNWRLGVENGFDASHIFIHKRSKLVAGNDIALPLGFAPIDAGDSTRAV
                     VGDTGPTGLYDLLGEKSIPVFEGSLGGEPVIEGHFGAKKVADNISIWLPGVLKVDPWP
                     QAGMSQFEWYVPVDATTHCYLQVLGRMTANKEEAAAFRQEVSDKWIELALDGFNDDDV
                     WAREATEEFYGDNDRGWIEERLFETDMAIVEWRKLASQRNRGIQR"
     gene            1386..>2205
                     /locus_tag="VNR65_12090"
     CDS             1386..>2205
                     /locus_tag="VNR65_12090"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HWJ19454.1"
                     /translation="MNVVWQSDVVPEQNQFASWREVICQHVYGVTPERVNGRGFRGKI
                     LARRFGGLDITDLECEGHLVSRRREDIARTASDTYYIYCQLAGSAWFSQSGRELVAET
                     GDIVIADPNVPFMTGTPGSFNFRIWRMPTKMLDPLRASSGQLMMTRLRHDDPICSVLS
                     SYLVALAIQAGRMGEIAEGYIADNVARLVALALGIAPDIQGSGRDALRQAKFARVLRY
                     IDSHLAEPDLLTPHAVSEKLGMSVRSLHLLFEIEGVSFGQWVTRRRLEEIKSLLT"
BASE COUNT          429 a          651 c          642 g          483 t
ORIGIN      
        1 cgctgtatgc cacgattgcg ctggctggct agtttccgcc actccacgat cgccatgtct
       61 gtctcgaaca atcgctcctc gatccagccg cgatcattgt ccccgtagaa ttcctccgtg
      121 gcctcgcgtg cccagacgtc gtcgtcgttg aaaccatcca gggcgagttc gatccatttg
      181 tcgctcacct cctggcggaa cgccgccgcc tcctccttgt tcgcggtcat gcgaccaaga
      241 acctgaaggt aacaatgcgt ggtggcatcg accgggacgt accattcaaa ctgactcatt
      301 ccggcctgcg gccacggatc gaccttgagt actcccggta gccagatcga gatgttgtcg
      361 gcgaccttct tggcgccgaa gtgcccttcg atcaccggct ctccaccgag cgagccttcg
      421 aataccggga tggatttttc gccgagtaga tcgtaaaggc cggtcggccc ggtatcgccg
      481 accacggccc gcgtgctgtc gccggcatcg atcggcgcga acccaagcgg caacgcgata
      541 tcgttgcccg ccaccaattt cgagcgcttg tgaatgaaaa tgtggctggc gtcgaagcca
      601 ttctcgacgc cgaggcgcca gttgctcttc accatccggc gcttgccgtg aatggccatt
      661 ccttcgtcga ggaagcccgg cgggacatcc gacgccagat cgggcggatc gatgtcgccg
      721 acgaaaacga aaatcagccc cttggcttcc tgggtcgggt aagtcttgag ctgatgcttg
      781 ccgatcagcg agctattggg attggtgatg atgtcggaaa gcttgccgtc atcccagttg
      841 taggtccaag catggtacca gcaggtaata gtccccttct tgtaacattc gatctgcttc
      901 gagaacttga cgccacggtg caggcaccga ttcctgatgg caagcggcct gccgtcgagg
      961 cgattgagga ggatgctttc gccgagcagc ttggcctcga cggggtgatc ctccagcagt
     1021 tcgtacgaaa acatggctgg ataccagtgg ttccggaaac caaatttggc ttcgacatac
     1081 ggtcgccatc cccgcacttt ttcgagcacg gccgggtcaa cgagcggttc tgacaccctg
     1141 atctctcctc acgctgattt tcggggccgc cagcaagcgg agccggatta gcatcgggca
     1201 ttttccgacg cttcatgccg ctgccgtcga tcctaaccgc cggtatattg ggaggcctgc
     1261 aatgactttg tcttcgcatt gagtgcaacg accatttgct ggcagcgcaa acttgcgctg
     1321 atcgcggccg gcgacggtac gactttgatt gacgacgatg gaccgcgcgc atagtgtgtc
     1381 aaaccatgaa cgtcgtatgg caaagtgacg tcgttccgga acagaaccag ttcgcttcgt
     1441 ggcgcgaagt gatctgtcag cacgtctatg gcgtgacccc agagcgcgta aacggcagag
     1501 gcttccgcgg caaaatatta gcacgcaggt tcggtggact ggacatcacc gatctggaat
     1561 gcgagggcca tctcgtctcg cggcggcgcg aggacatcgc ccgcacggca agcgatacgt
     1621 attatattta ttgtcaactc gcgggttcgg cttggttcag ccagtcggga cgcgaactcg
     1681 tcgccgagac aggcgatatt gttattgccg atccaaacgt tcctttcatg acgggtaccc
     1741 cggggagctt caattttcgg atctggcgca tgcccacgaa gatgctcgac ccattgcgtg
     1801 cgagcagcgg tcaattgatg atgacccgtc tgcggcatga cgatccgata tgttcagtgt
     1861 tatcgagtta tctggtcgcc ctggcgatcc aggctggccg catgggcgaa atcgccgagg
     1921 ggtacatcgc agacaatgtc gcccgactgg ttgctcttgc gctcggcatt gcaccggaca
     1981 tacagggatc gggtcgagat gcgcttcgcc aagccaaatt cgcgcgggtg ctgcgctata
     2041 ttgactctca ccttgccgag ccggatctct tgacgccaca cgcagtctcc gaaaaactcg
     2101 gaatgtcggt gcggtcgctg caccttctgt ttgagatcga aggcgtgagc ttcgggcagt
     2161 gggtaacacg gcggcggctc gaggagatca aatcgcttct tacca
//
LOCUS       DATMGX010000733         2550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_101608, whole genome shotgun sequence.
ACCESSION   DATMGX010000733 DATMGX010000000
VERSION     DATMGX010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101608"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VNR65_12095"
     CDS             <1..669
                     /locus_tag="VNR65_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWJ19455.1"
                     /translation="VYAMSESDHSATPSVESFSSELDTVTPSCPDPIRRAVILTALAA
                     GTCVLASKSAAIAEGDQPGSDERPQKADVLVISEGENEGKVIEPQDLKLGGPPLRAWP
                     KDPKTSVVRNGSRLNEILVVRLDPAELDDATRARSADGIVAYSAICSHAGCPVTAWVQ
                     AAAGDKDVLKCVCHNSEYDPRQGAQVVFGPAPRRLAALPLTTVGGSLTIAGTFVGKVG
                     GQQG"
     gene            752..2476
                     /locus_tag="VNR65_12100"
     CDS             752..2476
                     /locus_tag="VNR65_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HWJ19456.1"
                     /translation="MKQWLLSSLVASTCLVPIIAGAGPIENYSPVTADRLKNPEPGNW
                     LLYRRTYDGQGYSPLDQINTTNVKSLTPVWTFSTGVIEGHEAPPIINNGVMFVATPMG
                     QVIALNAKTGDEYWRYKRQLPDDLFQLHPTSRGVGLWQDKLYLATTDDHVVALDAKTG
                     KVVWDTKVQDYKKGQYLTLMPLVVDGKVIVGGSGGEFGVRGYVVAFDAETGKELWRTF
                     TIPGEGEPGHDTWSGDDWKSGGGSAWMTGTYDPDTKTIYWGVGNAAPWPGSLHPGDNL
                     YTSSVLGLDPDTGKIKAFHQYHQNDSWDWDEVDAPMLVDLQRDGRTFKSLIHPGRDAI
                     FWVLERKPDSINYVAGWPFVHTDVWKGIEPETGRPILDPDHKPVVGKRVEFCPSLWGG
                     KDWPSAAYSQKTKFVYVPANENFCGGFTGEKVPLVPGQLWLGTKPEDIGLKVRPGADH
                     YGELQAWDPATGKKVWSYNFPKSQLFGSVTATAGDLVLVGGTNDRIFRAFHAKTGELL
                     WEQKTNSGIMGMPIAYEVDGTEYIAVQSGWGVDAQRIQDALATTNNIGVENNVPQGGV
                     VWVFAVKK"
BASE COUNT          550 a          730 c          786 g          484 t
ORIGIN      
        1 gtttatgcca tgtccgaatc cgatcacagc gcgacaccat cggttgagag cttcagctcg
       61 gaactcgata cggtgacgcc gtcatgtccg gatccgatac gacgagcggt tatcctgacg
      121 gctctcgccg cgggaacgtg cgtcttggcg tccaaatctg ccgctattgc cgaaggggac
      181 cagcccggca gtgacgagcg gcctcaaaag gccgacgttc tcgttatctc cgaaggggaa
      241 aacgaaggca aagtcatcga gccgcaggat ctgaaactcg gcggaccgcc gctgcgcgcc
      301 tggcccaagg atccaaaaac ctcggtggtc cgcaatggct cgcggttgaa cgagatactg
      361 gtcgtcaggc tggatccggc cgaacttgat gatgcgacgc gcgcgcgctc ggccgacggc
      421 atcgtcgcct attcggcgat ctgctcgcac gcaggctgtc cggtcacggc ctgggtgcaa
      481 gcggccgcag gcgacaagga cgtgctcaaa tgcgtttgcc acaattccga atatgacccg
      541 cggcaaggcg cgcaagtcgt gttcgggcct gcgccgcggc gtctggcggc gcttccgctg
      601 acgacagtcg gcggttcact cacgatagcc ggaactttcg ttggcaaggt tggagggcag
      661 caggggtgat ggcgaccgcc atcgcatggc attccgtata actaagaaac aaataggtga
      721 gtgaaaacaa acgggaggaa acgttgtgac catgaagcaa tggctattat cgagtttagt
      781 agcatccaca tgtcttgttc cgatcattgc cggtgccggc ccgatcgaga actacagccc
      841 ggtgaccgcc gatcggctca agaatcccga acccggtaac tggctgcttt atcgccgaac
      901 ctatgacggg caaggataca gcccgctcga tcagatcaac accacgaatg tgaaaagcct
      961 gacgccggta tggaccttct cgaccggagt gatcgagggc catgaagccc cgccgatcat
     1021 caacaatggc gtgatgttcg tcgcgacccc gatggggcag gtgatcgcgc tgaacgccaa
     1081 gaccggcgac gagtactggc gatacaaacg ccagctccct gacgacctct tccaattgca
     1141 tccgaccagc cgcggggtgg ggctgtggca ggacaagcta tatctggcca cgacggacga
     1201 ccacgtggtc gcgctcgatg ccaagacggg taaagtggtc tgggatacca aggttcagga
     1261 ctacaaaaag ggccagtacc tgactctgat gccattggtt gtcgacggga aggtgatcgt
     1321 cggcggttca ggtggtgagt tcggcgtgcg tggttacgtc gttgcattcg atgccgaaac
     1381 cggcaaggaa ctgtggcgga ccttcaccat ccccggcgaa ggtgagccgg ggcacgacac
     1441 ttggagtggc gacgactgga aatcgggtgg cggatccgcc tggatgacag gtacctacga
     1501 tccagacacc aagacaatct attggggcgt cggcaacgcc gcgccttggc cgggctctct
     1561 tcaccccggt gacaacctct acacaagctc ggtgcttggg ctcgatcccg ataccggcaa
     1621 gatcaaagcg ttccaccagt accatcaaaa cgattcctgg gattgggacg aggtggatgc
     1681 gccgatgctg gtcgacctgc aaagggatgg ccgcacgttt aagagtctca ttcatcccgg
     1741 ccgcgacgcg atcttctggg tactcgagcg caagccagac agcatcaact atgtcgccgg
     1801 ctggcctttc gttcataccg atgtctggaa aggcattgag cccgagactg gcaggccaat
     1861 cctcgacccg gaccacaagc cggttgttgg caagcgggtt gagttctgcc cctcgctgtg
     1921 gggtggcaag gactggccat cggcggcgta tagtcagaaa accaaattcg tctacgttcc
     1981 ggccaacgag aatttctgcg gcggctttac cggcgagaag gtgccgttgg ttcctggcca
     2041 gctttggctc gggaccaagc ccgaagatat cggtctgaag gttcgtcctg gcgccgatca
     2101 ctacggcgag ttgcaggcgt gggatccggc caccggaaag aaggtctggt cgtataattt
     2161 ccccaaatcg caactgtttg gttcggtgac ggcgaccgcg ggcgatctcg tccttgtcgg
     2221 cggcaccaat gaccggattt tccgcgcctt ccacgccaag acaggcgaac tgctttggga
     2281 acaaaagacc aactccggca tcatgggcat gccgatcgct tacgaggtgg atggcacgga
     2341 atatatcgcc gtccagtcag gctggggcgt cgacgcgcaa cgcatccagg acgcattggc
     2401 gaccaccaac aatatcggtg ttgagaacaa cgtacctcaa ggcggcgtag tctgggtgtt
     2461 tgccgtcaag aagtgatcgc gagtaggcaa aatcctggcc gcttccttca ggcagcggcc
     2521 agggtcactt tctgaagctt tcaaaagctg
//
LOCUS       DATMGX010000734         2105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_209403, whole genome shotgun sequence.
ACCESSION   DATMGX010000734 DATMGX010000000
VERSION     DATMGX010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209403"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VNR65_12105"
     CDS             complement(<1..617)
                     /locus_tag="VNR65_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19457.1"
                     /translation="MRAAAIASLVLFLMALSYPAMSEGSEKWVTSWAASAQGPYPSGN
                     AMMQPDLRGAFPVPEAGAKDQSFRLIVGPDVWGPEARIRLSNAFGTKPVTFDGVFAGL
                     QLDSSAVVPGTNRPVTFAGAKNVTVQPGRSVWSDAIALPFTGALPGAYLQSRKLAVSF
                     HVAGASGPMTWHAKGLTTSYVTEPGAGSLGGNEDEAAFPYGTTSW"
     gene            complement(637..>2105)
                     /locus_tag="VNR65_12110"
     CDS             complement(637..>2105)
                     /locus_tag="VNR65_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ19458.1"
                     /translation="IEDTQPKVIISASCGIEPNRIVKYKPLLDQAIELSSAKPDYCII
                     VQRPQETAPLIEGRDYDYAALMEAAKGRGLSAPCEPMASTDPLYILYTSGTTGRPKGV
                     VRDTGGHMVALKWTMKAHYDIDPGEVFFVASDVGWVVGHSYIVYGPLLHGATTILYEG
                     KPVGTPDAGAFWRVISEHKVKAMFTAPTAFRAVKKEDPKGTLYGQYDLSNFKTLFLAG
                     ERADPNTIHWAEDLLKVPVIDHWWQTETAWAIAGNCVGLGAMPIKYGSPTYPMPGYEL
                     HVLNESNEEVKPGQTGALSIKLPMPPGCLPTLWNNDTGFVESYLIEYPGYYKTADAGY
                     IDEDGYVFVMARTDDIINVAGHRLSTGAMEEVVSNHPDVAECAVIGIHDGLKGQVPAG
                     FVLLKSGVERDISVIEREIVALVRDQIGPVAAFKTVLTVNRLPKTRSGKILRGTMRKI
                     ADGEQYKMPATIDDPAILAEITTALKAKGFPSAPSDAA"
BASE COUNT          402 a          639 c          633 g          431 t
ORIGIN      
        1 taccacgacg ttgtgccata ggggaaggca gcctcatcct cgtttccgcc gagcgatcct
       61 gcaccaggtt ccgtgacata ggatgtcgtg agcccctttg catgccacgt catggggccg
      121 ctcgcacccg ccacatggaa actgacggcg agctttctcg actggagata agctccagga
      181 agagcgccgg taaagggcag cgcgattgca tcgctccaaa cgcttcgccc aggttggact
      241 gtgacgttct tcgctccggc gaatgtcacg ggccggttcg ttccggggac aaccgcagag
      301 ctgtcgagtt gcagccccgc gaaaacgcca tcgaaggtaa ccggcttggt tccaaaggcg
      361 ttggagagcc ggatgcgggc ttcgggcccc caaacgtccg gcccaacgat gagccggaag
      421 ctttgatctt ttgcacccgc ttcgggaacg gggaaggcgc cgcgcagatc gggctgcatc
      481 atcgcattgc cgctcggata aggcccctgc gcggaggcgg cccaactcgt gacccacttc
      541 tctgatccct cagacattgc cgggtaagac aatgccatca agaaaagaac aagtgacgcg
      601 atggctgcgg cgcgcatggc ctctccgaat gtttgcttaa gcggcgtctg agggagcgga
      661 ggggaatcct ttcgccttca gcgccgtcgt gatttccgcc aggatcgccg gatcgtcgat
      721 cgtcgcgggc atcttatatt gctcaccgtc ggctatcttc cgcatcgttc cgcgcaggat
      781 tttgccggag cgcgtcttgg gaaggcggtt cacggtgaga accgtcttga acgcggccac
      841 cgggccgatt tgatcgcgca cgagcgcgac gatctcgcgt tcgatgacgc tgatatcgcg
      901 ctcgacgcct gacttaagga gcacgaagcc cgccggcact tgccctttca ggccgtcgtg
      961 gatgccgatc acggcgcatt ccgcgacatc gggatggttg gacacaacct cctccatcgc
     1021 gcccgttgaa agccggtgcc ccgccacgtt gatgatgtcg tccgttcggg ccatcacgaa
     1081 gacatagccg tcttcgtcga tataaccggc gtccgcggtc ttatagtagc ccggatattc
     1141 gatcaagtag ctttcaacga aacctgtatc gttgttccag agcgtcggca gacatccggg
     1201 cggcatcggt aacttgatcg acagtgcgcc tgtctggccc ggcttgacct cctcattgct
     1261 ctcgttgagc acgtggagtt cgtatcccgg catcgggtat gtgggcgatc cgtacttgat
     1321 gggcatcgcg ccaagaccca cgcagttgcc cgcgatggcc caagccgttt cggtttgcca
     1381 ccaatggtcg atgacaggaa ccttcaacag gtcttcggcc caatggatcg tgttgggatc
     1441 ggcgcgctcg ccggccagga acagcgtctt gaaatttgaa aggtcatatt ggccgtagag
     1501 cgtgcccttc ggatcttcct tcttgacggc gcggaacgcg gtcggcgccg tgaacattgc
     1561 tttgaccttg tgctcgctga tcacgcgcca gaaagctccg gcatccggcg tgccgacggg
     1621 cttgccttca taaaggatcg ttgtcgcgcc atgaaggagc ggcccgtaaa cgatataaga
     1681 gtggccgacc acccagccca cgtcggaggc tacaaagaac acttccccgg ggtcgatatc
     1741 gtaatgggcc ttcatcgtcc atttcagggc gaccatatgg ccgcccgtgt cgcgcaccac
     1801 gcccttgggc cggccggtcg tgcccgaggt gtaaagaata tagagcggat cggtggacgc
     1861 catcggctcg caaggtgcac tcaacccgcg gcctttcgca gcctccatca gagcggcata
     1921 gtcgtagtcg cggccttcga tgaggggcgc ggtctcctgc ggacgctgca cgataatgca
     1981 atagtcgggc ttcgccgagg acaactcaat cgcctgatcg agcaaaggct tgtacttcac
     2041 gatcctgttg ggctcgatgc cgcaagacgc agaaataata accttgggct gcgtatcttc
     2101 gatgc
//
LOCUS       DATMGX010000735         2265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_175919, whole genome shotgun sequence.
ACCESSION   DATMGX010000735 DATMGX010000000
VERSION     DATMGX010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175919"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VNR65_12115"
     CDS             complement(<1..909)
                     /locus_tag="VNR65_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19459.1"
                     /translation="MRDSSFFVRSTDLTRAKAALINGRPAAASYNSLIGIIERRAGAE
                     AASLFAEPVFPAGNDTDFKHIVSWYSVHEGAVVELDALDEAARKPAVNRLNQHLQSLS
                     PLLQDKQLGTALSSWLNLTSPKDILLIGGEPVLINWGFIPEEAAPSKDLRQHQFLRTL
                     GRFAEWLPLPALEASTPPSEAPLRRSDSNQFAVEGVQSMQDNSPGGDRNGAERPWLAP
                     LVASAIALAVLLVLLIPGVLRYPDANAGLAANRLEEERLRASNDSLEQQLKALEAEST
                     DRTCRPAGLSIPVPGLGNGQDPAKMEA"
     gene            1302..>2265
                     /locus_tag="VNR65_12120"
     CDS             1302..>2265
                     /locus_tag="VNR65_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19460.1"
                     /translation="MSLRVSDDDRPGYAILDFGRDTGTDDLLISLQSKLFNTGEYLGP
                     GGKWSKAPHFFKAVRLTSAGPGIYRIGPEIVNQQDLAHDTIEVSVGSGGVVEEATWPE
                     LTPAPFEPGTAIYREPVPPPPQEPIRPASKPAAESPPRVVEERPAKEDAGTPVTKTPA
                     WLKFGVPAAALFFLVMLSSWLFCTPFGFLCPTPVALDLAIKVAFQNAKSCAESNRADP
                     CAARQCFEEFLKDPQAPRENRADAERLAANLKLTCEAKKRDDADKDAAAKAETCIGEN
                     GRPPCRNSENCLAAYVASSPSGGERERLSRLARETFEKCKGEAVR"
BASE COUNT          492 a          656 c          676 g          441 t
ORIGIN      
        1 cgcctccatt ttcgcagggt cttgcccatt gccgaggccg ggcactggaa tcgaaagacc
       61 cgccgggcgg cacgtgcgat cggttgattc agcctccagc gccttcagct gctgctccag
      121 cgagtcgttg gaggcgcgca ggcgttcctc ctccaacctg ttggcggcaa gtcccgcgtt
      181 cgcgtccggg tagcgaagga cgccggggat gaggaggacg aggaggacag ccagcgcaat
      241 tgcgctggcc acaaggggcg ccagccatgg ccgttcagcg ccattgcggt ctccgccggg
      301 gctattatcc tgcatggatt gcactccttc cactgcaaac tggttggaat ccgatctcct
      361 taggggagcc tcagaaggcg gcgtagacgc ttcgagggca ggcaaaggca gccattccgc
      421 aaaccggcca agcgttcgaa ggaattggtg ttgccgcaaa tccttggagg gcgccgcctc
      481 ttcggggatg aatccccaat tgatgagaac aggctctcca ccgatcagaa gaatgtcctt
      541 cggtgaagtg agattgagcc aggaagaaag ggcggtgccc agttgtttgt cctgcagcaa
      601 cggggagagc gactgcaaat gctgatttag ccggttgacc gcaggcttgc gcgcagcttc
      661 atcaagcgcg tcgagttcta cgactgctcc ttcgtggacg ctgtaccacg agacgatatg
      721 cttaaaatca gtgtcatttc cagcgggaaa gaccggttcc gcgaaaagcg acgcggcttc
      781 agcccccgcg cggcgttcaa tgatgccgat cagcgaattg tagcttgccg ccgcaggccg
      841 tccgttgatg agagctgcct tcgcgcgcgt caaatctgta ctgcggacaa agaaagaact
      901 atcccgcatc gcgtcctgtg gaaatgtcaa cagtttgagt gcttagaaaa aaagaaaatg
      961 cgcgcgttta acgcagcccc tgataatagt gatccgtgta ttttggtcaa agcaattaca
     1021 atgacacttt catgacattc caacgcggtg acttgattga gattaaggca tgatcggggc
     1081 cggctcgccg atcccagccg cggcagccct gaatgaaagc ggaagttcat acgccgggac
     1141 attccgcagc aggagttgta ttttaccacg acgcgcctag aggctgtaaa gccggatatg
     1201 acagttccat gacatcgctt gacggtgaca cgactcttgg cgcgcgttat gagcattaag
     1261 ctgagtatcc gctaggcggg atccagcgct ggagatgcgg aatgagcctt cgagtttccg
     1321 atgacgatcg tcccggctat gcgatcctcg attttggacg cgataccggc acggatgatt
     1381 tgctcatttc gctgcagtcc aagctgttca atactgggga ataccttggc cccggcggga
     1441 aatggtcgaa agcgcctcac ttcttcaaag cggtccggct cacaagtgcc ggcccgggga
     1501 tctacagaat cggccccgaa atcgtcaacc agcaggatct cgcgcacgat accattgagg
     1561 tgagcgtcgg aagcggcggc gttgttgagg aagcaacctg gcctgaactc actccagcgc
     1621 ctttcgagcc ggggaccgcc atttatcgtg agccggtacc gcccccgcca caggagccaa
     1681 tccggcccgc atcgaagccc gctgcggagt caccaccacg ggtggtggaa gagaggcccg
     1741 ctaaagagga cgccggaacg ccggtaacca aaacgcccgc ctggctcaaa ttcggtgtac
     1801 ccgcggcagc cctatttttc ctcgtcatgc tgtcgtcatg gctgttttgc acgccgttcg
     1861 gtttcctgtg ccccacgcca gtcgcgcttg atctcgcaat caaagtcgcg ttccaaaatg
     1921 caaagtcctg cgcggagagc aaccgggccg atccctgcgc cgcgcggcag tgctttgagg
     1981 aatttctcaa agacccgcaa gcgccgcggg agaatcgtgc cgacgctgaa cgattggctg
     2041 caaatctcaa gctaacgtgc gaggccaaga agcgagacga tgcagataag gacgccgcag
     2101 ccaaggcgga aacttgtatt ggagagaatg gccgcccccc gtgccgcaac tccgaaaact
     2161 gccttgccgc ctacgttgct tcttccccct ccggtggtga gcgcgaacgg ctgtcgcgat
     2221 tagcgcgcga aacattcgaa aagtgcaagg gcgaggcggt acgca
//
LOCUS       DATMGX010000736         4223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_166918, whole genome shotgun sequence.
ACCESSION   DATMGX010000736 DATMGX010000000
VERSION     DATMGX010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166918"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..3221)
                     /locus_tag="VNR65_12125"
     CDS             complement(120..3221)
                     /locus_tag="VNR65_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWJ19461.1"
                     /translation="MNPSALFIRRPIGTILLSVGIFLAGVVAYFKLPVASLPSVDMPA
                     VRISAQLPGADPETVAATIAAPLERHIGEIAGVNELTSTSQLGSTSIQVQFDLSRNGD
                     DAARDVQAAINAARADLPTTLTRRPTMRKSNTAGAPIMILAMTSPIRRPTDLYDLGDL
                     IVSTRISQINGIAEVLIGGAQTPSVRVVADPGALQSRSLGFEDVRTAITQANSLVPIG
                     TLNGPEKSTIVTTNGQIDKPSDYASLVLKTKSGNVVRIGDIAQVSTGTSNRLASGYFN
                     KDNAVIFIIFKTPDANVVNTVDQIYALFPELHRLLPADVKLEVLNDRTKTIKASIADI
                     QMTLLASIALVMMVVFIFLRRIIPTVAAGIAVPLSLAGTVGLMWLSGYSLDNISLLAL
                     TISVGFVVDDAIVVIENCYRNMEAGMKPLEAALQGSRQIGFTIISISLSLVAAFIPLL
                     FMGGLIGRLLGEFAWTLTYAILISAVVSLTLTPMICGRFIRRLPRPRETWLDRRIEPF
                     FEGMIRAYERSLTYALRHQWLMLLITLTTLALTINLYIVLPKDVIPAGDTGLVIGYAQ
                     AAPEVSFDALEGIQKRVTEILDTDPAVDGVAVSIGGTSGFGQSNQARFYMSLKPIEER
                     KESAMAVIDRLRKKLAAVKGVDVRMNPGQEIQFGGRPSQAKYQFTLWGTDLKEIAEWL
                     PKLQERVKQVPGVVDVNNDRQAAGPQAMVTIDRVKAGKFGVSVKDIDAALNDAFSQRA
                     VSTIYSYRNQYNVVLEANLDVQRSLEGLNTVYVKGSQGQQVPLSSIATISVTSMPLVV
                     NHTGQFPAATISFNTAPTVTLGQVQPQILQIAQDIQLPESLHFEAAGETLALQQQQSS
                     MVLLIVAALVAVYLVLGILYEDLVHPLTILSTLPSAGFGALLTMQATGQSLSVIAFIG
                     IILLIGIVKKNGIMLVDFALDAERSRGLSSEDAIRQACLERFRPILMTTLAAMFGALP
                     LVLAAGPGSELRTPLGYTIVGGLAVSQFLTIYTTPVIYLWLDRLRRRGRRRQAPSVVA
                     APS"
     gene            complement(3226..>4223)
                     /locus_tag="VNR65_12130"
     CDS             complement(3226..>4223)
                     /locus_tag="VNR65_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWJ19462.1"
                     /translation="PKFQTDLAALGSIYVPGNAISNSTTGNSVAGLSSGSSGPTGSSI
                     TAVPTIGQGRGAQVLLSSFATIERSVAPLSVNHVQQYPAATISFDVAPGVSLDAAVQA
                     VADATARLGMPSSIVGSYTGAAAEFNASLKSTPFLVLAALVTIYIILGVLYESFIHPF
                     TILTTLPSAGIGALLALNYFGMEFSFIALIGVILLMGIVKKNAIIMIDFALDAERHRG
                     MTPFEAIREACLLRFRPIMMTTVAALLGALPLVLGSGPGAELRTPLGVTIIGGLLLSQ
                     FLTLYTTPVIYLAMDGLKRRIERLLGMGEPAAAPPGGSAGGPPPAGAPAAPETRG"
BASE COUNT          890 a         1205 c         1329 g          799 t
ORIGIN      
        1 aacgattggc taaaattggt tttgtttcgt aaaaccactt ataagtaaat atataagtaa
       61 ttttcccgcc ataaaagagc atccagaggc aaaggtgtcc tgacgtatgg cggttcgttt
      121 tacgagggtg ctgcgacaac gcttggcgct tgccgtcttc ttccccgcct acgcaggcga
      181 tcgagccaaa ggtagatcac aggggtcgta tagattgtca ggaactggga gaccgccagc
      241 ccaccgacga tggtatagcc gagcggcgtt ctaagctccg agcccggccc ggcagcaaga
      301 accagcggca aagcgccaaa catggctgct aacgtggtca tgaggatcgg gcgaaatcgc
      361 tcgaggcaag cctggcggat tgcgtcctcg ctggacagcc ccctgctacg ctccgcgtcc
      421 agcgcaaaat cgaccagcat gatgccgttc tttttcacaa taccgatgag aagaataatg
      481 ccgatgaagg cgataactga gagggactgc cctgtcgcct gcatcgtcag caacgcgccg
      541 aagcctgccg acgggagcgt cgacaggatg gtgagagggt ggacaaggtc ttcatagagg
      601 atgccgagca cgagatacac cgcgacaagc gccgcaacga tcagcagcac catggaggat
      661 tgctgctgct gaagggctaa cgtctctccc gcggcctcga aatgaagcga ttcgggaagc
      721 tggatatctt gggcgatttg caggatctgc ggctgaacct gtccgagcgt aacagtcggt
      781 gcggtgttga agctgatcgt cgcggcgggg aactgccccg tatggttcac gacgagcggc
      841 atggacgtga cgctgatcgt cgcaatggag ctaaggggca cctgctgccc ctggctgcct
      901 ttcacgtaga cggtgttaag cccttccagg ctgcgctgca cgtccagatt cgcttccagt
      961 acgacgttgt attgattgcg gtacgaatag atggtcgata ccgcacgctg cgagaacgcg
     1021 tcgttgagcg cggcgtcgat gtccttgacc gaaacgccga atttgccggc tttcacccgg
     1081 tcgatagtca ccatcgcctg cgggccggcc gcctggcgat cgttgttgac atcgacgacg
     1141 cccggcactt gcttgacacg ctcttgcagc tttggaagcc attcggcgat ttcctttaaa
     1201 tccgtccccc aaagcgtgaa ttgatatttc gcctgagatg gccgtcctcc gaactgaatt
     1261 tcctggcccg gattcatcct cacatcgacg cccttcaccg cggccaactt cttccgcaac
     1321 cggtcgatca cggccatcgc ggactctttg cgctcctcga tcggcttcag cgacatgtaa
     1381 aagcgggctt gattgctttg tccgaagccg gacgtcccgc cgattgatac ggccacgcca
     1441 tccacggcag ggtcggtgtc caaaatctcc gttacccttt tttgaatacc ttccagagcg
     1501 tcgaaagaaa cctctggagc agcttgggca tacccgatga caaggcctgt gtcgcctgcc
     1561 ggaatgacgt ctttcggcag cacgatgtag aggttgatcg tcagcgccag cgtcgtcagg
     1621 gtgataagaa gcatcaacca ttggtgccga agcgcgtagg tgagcgagcg ctcgtaagcc
     1681 cttatcatcc cttcgaaaaa gggttcgatc cggcggtcga gccaggtttc tcgcgggcgc
     1741 ggcaagcggc ggatgaagcg gccgcaaatc atcggcgtga gagtgagaga caccaccgca
     1801 gaaataagga tcgcgtaagt cagcgtccac gcgaattcgc ccaaaagccg gccgatcagg
     1861 ccacccatga acagaagcgg gataaaagcg gcaaccagcg acaggctgat ggatatgatg
     1921 gtaaagccga tctgccgcga gccttgcagc gcggcctcca gcggcttcat ccccgcttcc
     1981 atgttgcggt agcagttctc gatcacgacg atggcgtcat cgacgacaaa accgacggag
     2041 atggtcagag cgagaagcga aatgttgtcg aggctataac cggaaagcca catgagcccc
     2101 acggtgcccg cgagcgaaag tggcaccgcg atgccggccg cgaccgtcgg aatgatgcgg
     2161 cgaagaaaga taaaaaccac catcatgacc agcgcgatgc ttgcgagaag cgtcatttga
     2221 atgtcggcga tgctcgcctt gatggtcttg gttcggtcgt tcagcacctc aagtttcacg
     2281 tctgccggaa ggagccggtg aagctcgggg aacagagcgt aaatctggtc gacggtattg
     2341 acgacgtttg catcgggcgt cttgaagatg atgaaaatga cggcgttatc tttgttgaaa
     2401 tagccagacg cgagcctgtt gctcgtgccc gtggaaacct gagcgatgtc gccgatgcga
     2461 acaacattgc cactcttcgt cttcaggacc agcgacgcat aatccgatgg tttgtcgatc
     2521 tggccgttgg tcgttacgat cgttgatttc tctggtccgt tcagcgtccc gatcggaacg
     2581 agcgagttgg cttgcgttat ggcggtgcgg acatcttcaa agccaaggct gcgcgattgc
     2641 agcgcccctg gatcagcgac gacgcgcacc gaaggcgtct gcgctcctcc gatcagcacc
     2701 tcggcgatgc cgttgatctg gctgatacgc gtcgagacga tgaggtcgcc gagatcgtaa
     2761 agatcggtag gccgcctgat cggagaggtc atagccagga tcatgattgg cgcgccggcg
     2821 gtgttcgact ttctcatcgt cggccggcgg gtcaaagtcg tcggaaggtc ggcccgcgcc
     2881 gcgttgatgg ctgcctgcac atcgcgggcg gcgtcgtctc cgttccgcga caggtcgaac
     2941 tgaacctgga tgcttgtgga gccgagctgg ctcgtggacg tcagctcgtt gacgcccgca
     3001 atctctccga tgtgccgctc aagcggagcg gcgatggtgg ccgctacggt ctcgggatcg
     3061 gcgcccggca attgcgcgct gatccgcacc gcgggcatgt cgacgctggg aaggctcgcg
     3121 accggcagct tgaaataggc gacgacgccc gcaagaaaga tcccgactga aagcaggatg
     3181 gtcccaattg gccgccggat gaagagcgct gacgggttca tgggcctagc cccgggtttc
     3241 cggcgcggct ggcgcgcccg ctggcggagg cccccctgcc gatccgcctg gaggcgcagc
     3301 ggcgggctct cccatgccga gtagccgctc gatgcggcgt ttcaagccgt ccatggcgag
     3361 gtagatcaca ggcgtcgtat agagggtgag gaattggctc aacagcaaac cgccaatgat
     3421 ggtgacgcca agcggcgtcc gcaactcggc ccctgggccg ctgccgagca cgagcggaag
     3481 cgcgcccaag agcgcagcga cggttgtcat catgatcgga cgaaagcgaa gcaggcaggc
     3541 ctcccggatg gcctcgaacg gcgtcatgcc ccggtgacgt tcggcatcaa gcgcgaagtc
     3601 gatcatgatg atggcgttct tcttcacgat gcccatcagg agaatgacgc cgatgagcgc
     3661 gatgaacgag aattccatgc cgaaatagtt gagagcgagc agcgcgccga tgcctgcgga
     3721 gggaagcgtc gttagaatgg tgaaggggtg aatgaaactc tcgtacagca cgccgagaat
     3781 aatgtagatc gtcacgagcg cggcgagcac gagaaacggc gtacttttca gcgaggcatt
     3841 gaactcagcc gcggcgccgg tgtagctgcc gacaatcgag cttggcatgc caaggcgggc
     3901 cgtcgcatcg gctacggcct gaacggcggc gtcgagagag acgccgggcg caacatcgaa
     3961 gctgatggtc gcggcgggat attgctgcac gtggttgacg gatagcggcg caacgctccg
     4021 ctcgatcgtc gcaaaggacg acagcagaac ttgcgccccg cgcccctgcc cgatggtggg
     4081 cacggccgtg atggacgaac cggtcggccc gctgctcccg ctggaaaggc cggcgaccga
     4141 attgccggtc gtggagttgc tgatggcatt gccggggaca tagatggacc ccagcgccgc
     4201 aagatccgtc tggaacttcg gat
//
LOCUS       DATMGX010000737         2035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_300769, whole genome shotgun sequence.
ACCESSION   DATMGX010000737 DATMGX010000000
VERSION     DATMGX010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300769"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VNR65_12135"
     CDS             complement(<1..737)
                     /locus_tag="VNR65_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19463.1"
                     /translation="MQIAARSLVRVVLVLSVFGLVTPCFAVEKQRIEVNDYAIQAVIS
                     PQTHQIKAQARVRFTALDDITIAIFELHDALRPTRVLEASGQPLSVERVSQDSTIRIS
                     LPNGLTKGSSDTLTFEYEGAIQSGDDSPVPGLKLAYIGDPTTYLLYAGRWFPMVGYGI
                     DRFTSTISISVPSEYSVIGSGKQTSGAAPEAGADASARKGSTAHVPLGKLAVPTGYKT
                     VTFSYEDRPSFPGTILIGKYEQTKNNEG"
     gene            complement(962..>2035)
                     /gene="lpxB"
                     /locus_tag="VNR65_12140"
     CDS             complement(962..>2035)
                     /gene="lpxB"
                     /locus_tag="VNR65_12140"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HWJ19464.1"
                     /translation="HQTSAPDLEFFGVGGDQMRAAGCDLLVDAREIAEVGIVEVIKHI
                     PLIYRRFREVVREAERRRPDAAVLIDFPDFNLRLARKLHKLGVPVIYYVSPQLWAWKQ
                     GRIEQVRKYVRKMLVIFPFEERFYRERGIEAQFVGHPLADLPPPQVTREQFAAEHKLD
                     PAREWIALLPGSRRGEVSRIFPTLLEAAQLLGPEYVYIVPVASTLDAAWVASFLRDSA
                     GPRITFTGDARASLLHARAAAVASGTSTLEAALIGTPFTMVYRVAPLTWSLGRRLVKV
                     DRFAMVNLIAERDVVPELVQGEFTPQRVCAELRRLIPEGEARETMIAGFGEVRRRLRP
                     SDPGGNASERAAQAVLALLGSSG"
BASE COUNT          412 a          616 c          621 g          386 t
ORIGIN      
        1 ccttcgttgt tcttggtctg ctcgtacttg ccgatcaaaa tggtccctgg aaagctcggc
       61 ctatcttcgt agctgaaggt gacggtcttg tatccggtgg gaacagccag cttgccgagc
      121 ggcacgtggg cagtactgcc cttgcgcgca ctggcatcgg ccccagcttc cggagcggcg
      181 ccggaagtct gcttgccgct gccgataacg ctgtactcgc tgggtacgct gatgctaata
      241 gtcgaagtaa agcgatcgat gccatagccg accatgggga accaccgccc ggcgtagagc
      301 agatacgtcg tgggatcgcc gatgtaggcg agcttcagcc ccggcactgg gctgtcatct
      361 cccgactgga ttgccccttc gtattcgaag gtcagggtgt cgctgctgcc cttggtcaac
      421 ccattcggca aggagatgcg gatggtcgag tcctgactga cccgctcaac cgaaaggggt
      481 tggccgctgg cctcgagcac gcgcgtcggc cgcagcgcat cgtgtaattc aaagatggca
      541 atggtgatgt cgtcgagcgc ggtaaagcgg acgcgagcct gtgccttgat ctgatgggtc
      601 tgcggagaga tgaccgcctg gatggcgtag tcgttgacct cgatccgctg cttctcaacc
      661 gcgaagcacg gggtaaccag accgaaaacc gaaagcacca ggacgacgcg cacaagagat
      721 cgcgctgcga tttgcattag ggaggttgct cctgctctgc tgctcactat aaatgatgag
      781 aaaccagccg caaaggtgcg cacggatcta ccacagagac agagagaagt ttcgagtttc
      841 cagtttcgag tttcaagttg tgaactggaa cgatgcaact ctaaagaatt ctctgtgtct
      901 ctgtggtgaa tgttttcaaa ttggccggct caccctgacg gtccgaaact cgaaacttct
      961 ctcatcctga cgatcccagc aatgcaagta cggcctgggc cgcccgctcg gatgcattgc
     1021 cgcccggatc gctgggccgc aagcgacgcc gcacttcgcc gaagcccgcg atcatggtct
     1081 ctcgcgcttc tccttctgga atgagccgcc gaagctcggc gcaaacccgt tgcggcgtga
     1141 actcgccctg gaccagttcc ggaaccacgt cacgttccgc gatcaggttc accatcgcga
     1201 agcgatccac ctttactaga cggcggccga ggctccaggt gagcggcgcg acccggtaca
     1261 ccatggtgaa cggcgttccg atcaaggctg cctcgagcgt ggacgtgccg ctggcgactg
     1321 ctgccgcccg cgcatgcagc aaactggccc gggcgtcgcc cgtaaacgta attcgcggcc
     1381 ccgcagagtc gcgcaagaaa gaagctaccc aggctgcatc gagggtggaa gcaactggaa
     1441 ctatgtaaac gtattctggg ccgagaagtt gcgctgcttc taatagtgtc gggaagatgc
     1501 gcgaaacttc gccgcgccgg ctgccgggca acaaggcaat ccattcccgg gccggatcaa
     1561 gcttatgttc cgcagcaaac tgctcccgcg tcacctgcgg aggaggcagg tcggcaagcg
     1621 gatggccgac aaactgcgcc tcaataccgc gctcgcggta gaagcgctcc tcgaaaggga
     1681 aaatcaccag catcttgcga acgtatttcc gcacctgctc gatgcggccc tgcttccacg
     1741 cccagagctg cgggctgacg tagtaaatga cgggcacgcc gagcttgtgc agcttgcgcg
     1801 ccaggcgcag gttgaaatcg gggaagtcga tgagcaccgc ggcgtctggc cgccgccgtt
     1861 cggcctcgcg caccacttcg cggaagcggc gatagatgag cggaatgtgc ttgatgacct
     1921 cgacaatgcc gacttcggca atctcgcgcg catccaccag aaggtcgcaa ccggcggcgc
     1981 gcatctggtc tccgccgacg ccgaagaact ccagatcggg cgccgaagtt tggtg
//
LOCUS       DATMGX010000738         4212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_393016, whole genome shotgun sequence.
ACCESSION   DATMGX010000738 DATMGX010000000
VERSION     DATMGX010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393016"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VNR65_12145"
     CDS             <1..935
                     /locus_tag="VNR65_12145"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HWJ19465.1"
                     /translation="ENAVLRHQLIVLRRKVQGRVRLTNNDRWFLIQLYRWFPSILQVL
                     MIVQPDTLVRWHRAGFRYYWRWKSRPRGGRPQIETDLRALIRRMSMENPLWGAPRIHG
                     ELLKLGFEVAQSSVAKYMAKRRGPPSQGWRTFLRNHAPDIAAMDLFVVPTIGFDLLYA
                     FVIVRLDRRDLVWINVTTNPTAEWIARQLIEAFPWDEAPRYLIRDRDRIYGTIVTRGM
                     RSMGIRDKPTARASPWQNGFAERLIGSIRRECVDHFIVLGEAHLRRILRAYVGYYNDI
                     RTHRSLDKDAPVSRSVQRTGIINSRPILGGLHHH"
     gene            1014..1208
                     /locus_tag="VNR65_12150"
     CDS             1014..1208
                     /locus_tag="VNR65_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19466.1"
                     /translation="MQTITTIGLDIAKSVFQVHGVDAQGSPKDCSASSSNPMMEGPLV
                     PIPKRSTSPRATSTLQWWTV"
     gene            1318..2991
                     /locus_tag="VNR65_12155"
     CDS             1318..2991
                     /locus_tag="VNR65_12155"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWJ19467.1"
                     /translation="MRGNEQRLLSEVAIVGGGPVGLMLALFLDRYGIRTIVFNVEDHV
                     RMHPKGSSHNSRTMEHYRRLGISEPIRRLGLPADHPKDVAYFTRLSAWEVARFRMPSD
                     IEVAHLTKVAMQTDQIPEPMHRGNQMYVEEFLLKHARTRPNITLRFGWEAADFSQTAD
                     AISLTAVRAADGQTEHWDAGYLIGCDGGHSAVRRSLGIRYEGYDKIEQAYLGGRMTST
                     YLRAPTLYREHLGRRRAWIYWVINPELRSTLFALNGKDEFMFWTKPADANAPPDEAAV
                     KQALYRCVGAEIPVEFISHQPWTAGAALVAERFADRRIMLAGDAVHLFTPTGGFGMNT
                     GVDDAANLSWKLAALLRGWGGNSLLDSYELERRPIATRNTRFARALAKQVGIVEIPAG
                     MEDDTPSGAVQRQEVGAFVSTYLGAQFAPIGVELGAHYSGSPIIASEEAAPTDSIVQY
                     TPTSVPGGRTPHVWLGAGRGIGDSLFDRLGAAFTILRLGPNPPSAASLATAAQDRGVP
                     IEIIDLPDAAVRDLYDCDLALVRPDQHIAWRGEASPADAGVVLDCVIGA"
     gene            complement(3167..3856)
                     /locus_tag="VNR65_12160"
     CDS             complement(3167..3856)
                     /locus_tag="VNR65_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWJ19468.1"
                     /translation="MGAIEVTYFSDVLCIWAYASQARVDAVKEQFGDAVQIKHRFCSV
                     FGDTAGKITSTWKDKGGYDGFNSHLRKVAEQFPHIEVHPEIWLKTRPSTSSSAHLFMK
                     AVQQWDRAYRVARTPNAGNIFDQVLWEFRCAFFRDCRDIACWDVQCEIAKAHGVDIVA
                     IEQCIHDGDAFARLAADYQDADKMRIEGSPSFVLNEGRQKLYGNIGFRLIEANIQELL
                     REPRTDEASWC"
BASE COUNT          876 a         1210 c         1183 g          943 t
ORIGIN      
        1 cggagaatgc ggttctccga catcagttga tcgttttgcg gcgtaaggtg cagggcagag
       61 tccggctgac gaacaacgac cgctggttct tgatccagct atatcgatgg tttccgtcga
      121 ttttgcaggt cctcatgatc gtccaacctg acacgctcgt gcggtggcat cgggccggct
      181 ttcgctacta ttggcgttgg aaatcgcgcc ctcggggagg gcggccgcag atcgagacgg
      241 acctgcgggc gctgatccgg cggatgagca tggagaaccc gctttggggc gcgccgcgta
      301 tccacggcga actgctcaag ctcgggtttg aagtcgcgca atcgagcgtc gccaaataca
      361 tggccaagcg ccgcggaccg ccaagtcagg gatggcgaac ctttctgcgg aaccacgcgc
      421 cggacattgc tgccatggat ctgttcgttg tcccgaccat tggcttcgac ctgctctatg
      481 ccttcgtcat cgtccgtctg gaccgcagag acttggtctg gatcaacgtc acaacaaacc
      541 caacggctga atggatcgca cgccagttaa tcgaggcctt cccctgggat gaggcgccgc
      601 gctacctcat ccgcgatcgg gatcggatct acggcaccat cgtcacacga gggatgcgct
      661 ccatgggcat ccgggacaag cccaccgcgc gggcctcgcc ttggcagaat ggctttgccg
      721 aacggttgat cgggtctatc cggcgcgagt gcgtggacca tttcatcgtc ttgggtgagg
      781 cgcatctgcg ccggatcctg cgagcctatg ttggctatta caacgacatc agaacgcacc
      841 gatcattgga caaagatgcg ccggtctcgc gctccgttca gcggaccgga attataaatt
      901 cacgccctat ccttggcggg cttcatcacc actaggtccg gatttaggtt ttcggtacac
      961 acagccatga atgtgggtgc tgttaaggcc cccacgattc agaggagcca aacatgcaga
     1021 cgatcacgac aattggtctc gatatcgcca agtcggtttt tcaggtccac ggcgttgatg
     1081 cgcagggcag tcccaaagat tgttccgcct cgtcttcgaa tcccatgatg gaagggcccc
     1141 tggtgccgat tccgaagaga agcacgagcc cgcgagcgac atcgacactg cagtggtgga
     1201 cagtctgaaa gcgcttgacc ccaaccggcc gattagagaa gcggacattg gcccgcgcta
     1261 ttgcaatgcg atgttcatga cgggtacaga tattcccgtg ggggcgcttg agcaatcatg
     1321 aggggcaacg aacaaagact gctgagcgag gtggcgattg ttggcggagg accggttggc
     1381 ctcatgctgg cactgtttct cgatcgctac ggaatccgaa cgatcgtctt caatgttgaa
     1441 gaccatgtgc ggatgcaccc caaaggcagt tcgcataatt cgcgtacaat ggaacactat
     1501 cggcggctcg gtatttccga acccatccgc cgcctgggtc taccagcgga tcacccaaaa
     1561 gacgtcgcct atttcacgcg tctctcagct tgggaggttg cgcgctttcg catgccctct
     1621 gacatagagg tggcgcattt gactaaggtt gcgatgcaaa ccgatcagat tccggagccc
     1681 atgcatcgcg gtaatcagat gtatgtcgag gagttccttc tcaagcacgc gcggacacgt
     1741 ccgaatatca ccttacgttt cggatgggag gcggctgatt ttagccaaac tgccgatgct
     1801 atttcgctta cagctgttcg cgccgccgac ggacaaaccg aacactggga tgctggttac
     1861 ctcattggat gcgatggcgg ccatagcgca gttcgtcggt cactcggcat ccgatatgaa
     1921 ggttacgaca agatcgagca ggcctatttg ggcggacgca tgacatctac ttatctgcgc
     1981 gcacctaccc tctatcgcga acatctcggc agacggcgcg cctggatata ctgggtcata
     2041 aatcctgagc tgcgcagcac gttgttcgcg ctcaacggca aagacgaatt tatgttttgg
     2101 accaagcctg cggacgcaaa tgcgccaccc gatgaggctg cggtcaaaca ggcgctgtac
     2161 cgttgcgtcg gagctgaaat tcccgtcgag tttattagtc atcaaccgtg gacagctggc
     2221 gctgctctag ttgccgagcg gtttgctgac cggcggatca tgctggccgg cgatgccgta
     2281 catctattca cgcccaccgg gggattcgga atgaacaccg gagtcgatga tgcggcaaat
     2341 ctgtcctgga agcttgccgc actgctgcga ggctggggcg gaaattcctt gctcgattcc
     2401 tatgagctcg aacgtaggcc catcgctacg cgcaatacga ggttcgcacg cgccctcgca
     2461 aagcaggtcg ggatagttga aattcctgct ggaatggagg acgacacgcc gtccggcgcg
     2521 gtgcaaagac aggaagttgg cgctttcgtt tcgacctatc ttggcgctca attcgcgccg
     2581 atcggcgtcg aacttggggc acattatagc ggctcaccta tcatcgcgtc ggaggaagcc
     2641 gcgccgaccg acagcattgt gcaatataca ccgaccagcg tgcctggcgg ccgcactccg
     2701 catgtttggc tcggagcggg gcggggcatc ggagattcgc tatttgaccg cttaggcgcg
     2761 gctttcacaa ttttgcgcct tggacccaat ccgccttccg ccgcaagtct agccacagca
     2821 gcacaggatc ggggcgtgcc gattgaaatc atcgacttgc cagacgcggc ggtgcgggac
     2881 ttgtacgact gtgacttggc tctcgttcgg cctgatcagc atatcgcctg gcgcggcgaa
     2941 gcgtcaccag cagacgccgg tgtcgtcttg gattgtgtca ttggtgccta ggcggcgccc
     3001 caaatcgtat caatttgcgg aacgcgattt tgtctgctct tggcacatcg cgtcattcgt
     3061 ggcgctgcag cattctcggt cgctattgag gcaacaacgg acatcgatcg gcacgagcgc
     3121 tggattgctc ggtcgcgaat gacccaaagc ggacattcgt tggcggtcaa caccaactgg
     3181 cttcatcagt gcgaggctcc cgaagaagtt cttgaatatt tgcctcgatc aagcgaaacc
     3241 cgatatttcc gtatagcttc tggcgcccct cgttcaacac gaagctcgga ctgccttcga
     3301 tccgcatctt gtcagcgtct tgatagtccg cagccaacct cgcgaatgcg tcaccatcgt
     3361 gaatgcattg ctcgatagcg acgatatcga cgccatgggc ttttgctatc tcacattgaa
     3421 cgtcccagca cgcaatgtcc cggcagtctc ggaaaaacgc acagcggaat tcccacagta
     3481 cttgatcgaa aatgttcccc gcattgggcg ttcgtgccac tcgataagcg cggtcccatt
     3541 gttggactgc tttcatgaat agatgtgcac ttgacgacgt cgaggggcgc gttttcaacc
     3601 aaatctctgg atgaacctcg atatgaggaa actgctccgc caccttccgc aaatgcgagt
     3661 taaatccgtc atatccgcct ttgtctttcc aggtcgatgt gatcttaccg gctgtatcgc
     3721 cgaagacgga gcagaaccga tgttttatct gaacggcatc gccaaattgc tctttgacag
     3781 catccacgcg agcctgcgag gcgtatgccc agatacataa gacatccgag aaatatgtaa
     3841 cctcgatcgc gcccatctta ttattccctt tgtttccgtc atcgccctca acgtcggtcc
     3901 gtgctgcgtt ataagtctat cttctagtgc ctttcgttcc aatttgatcg cgcaactgtc
     3961 ttcatttgaa tttggactgg aaccatttcg agccggcgga acttgtcctt ccgccaacat
     4021 agaattccta gtcaagaccg gcagcgctct ttgccgcagg cggcgaagcg tccgtaaagt
     4081 ctattgctca ggtctaggct atggctcgca catcaagccg cgctatgcgc gcgaaggaat
     4141 caaacatgcg taaaattctg cttctcacga cattggccgc ggccgcagtg gtcgccgccg
     4201 gaatcacggt ac
//
LOCUS       DATMGX010000739         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_321116, whole genome shotgun sequence.
ACCESSION   DATMGX010000739 DATMGX010000000
VERSION     DATMGX010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321116"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VNR65_12165"
     CDS             <1..544
                     /locus_tag="VNR65_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWJ19469.1"
                     /translation="TTTASLNSSGAIYGGQIGCDYQFAPSWVVGVEGAVSAGSLDGST
                     RVPFQPLVAPGDTELVRVRTDFIPTVTARLGYAMDHWLFYGKGGIAWTNDKYSVTGVF
                     QGTPFDAEGLGMRTGWTAGAGVEWAFTDDWSARLEYDYYDFGHSNVLMSDPTNGFASI
                     VTYRQSAQMVKLGLNFRVGP"
     gene            638..1285
                     /locus_tag="VNR65_12170"
     CDS             638..1285
                     /locus_tag="VNR65_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ19470.1"
                     /translation="MKILGFKQTGWKAHLKMAIIAAAFAAVSPSTGQADELQAKVQYC
                     KTCHGLSGQGYHGYFPMPRLAGQQPQYIENQLRAFIEHRRKNLIMANVAHALSPSMLS
                     ALAHHFKGLNPPPLGGGPRGDVTTGKRIYEEGVPESNVPACSACHGTDAHGQDQIPRL
                     AGQLPDYFYSKLVNWTSERGQNTSMPDSSAVMGPTSHNLTKSQISAVAAYVSNLR"
     gene            1346..1936
                     /locus_tag="VNR65_12175"
     CDS             1346..1936
                     /locus_tag="VNR65_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ19471.1"
                     /translation="MKRIGLKLTILAILVSSWAGGSKAASIRNCTWCHGTGAQGYAAA
                     PRLAGQRSMYIEAQLRGYAAHIRDNPFSKQYMWGAVANLRSDAVRDLADYFASLPPKA
                     AHDGRRALVDKGQTIYDQGIPEANIVSCLACHGPGGQGVREIPRLGGLSYFYLKRRLE
                     EWGRGYHSAAYPMPTVASTLGPDEIEALASYLSFVK"
     gene            2259..2594
                     /locus_tag="VNR65_12180"
     CDS             2259..2594
                     /locus_tag="VNR65_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWJ19472.1"
                     /translation="MKKICLTVLMVCAWGNASYALDREQQHGKELLGTLCGRCHAVGQ
                     TGRSPHVDAPPFRTLGDSKLYDNDFAARLEEGLSTIHPDMPTFRFSREDAEASVNYLK
                     SIQGHKQPK"
BASE COUNT          552 a          815 c          835 g          540 t
ORIGIN      
        1 aacgaccacc gccagtctca actcgagtgg cgcgatctat ggcggccaga tcggatgcga
       61 ctatcagttt gcccccagct gggtggtcgg cgtcgagggc gcggtctccg ccggatcgtt
      121 ggatgggagc acgcgggtgc cgtttcagcc cttggtggcc cccggagaca ccgagttggt
      181 cagggtaaga accgatttca tcccaaccgt caccgcgcgg ctcggctatg cgatggacca
      241 ctggctattc tacggcaagg gcggcattgc ctggaccaac gacaagtaca gcgtgaccgg
      301 cgtgttccag ggcaccccgt tcgacgccga gggactgggc atgcgcaccg gatggactgc
      361 gggcgctggc gtcgaatggg ccttcacgga cgattggtcg gcaagactcg aatatgacta
      421 ctacgacttc ggccactcga atgtgctgat gtccgacccg accaacgggt tcgcgtcgat
      481 cgtgacctac aggcagagcg cgcagatggt caagctgggg ttgaattttc gtgttgggcc
      541 gtagggtcgg cgagagagtc caattggcgc gcagcagtcc gggcgcgaag ttttcggcag
      601 gatcatgagt ctccagcgta acggcgaaag cgagataatg aagattctcg gttttaagca
      661 aactggctgg aaggcgcatt tgaaaatggc gattattgcg gcggcattcg ccgctgtatc
      721 gccatcgaca ggtcaagctg atgagctcca ggccaaggtg caatattgca agacctgcca
      781 cggtctgtcg gggcagggtt atcatggcta ctttcccatg ccccggctcg cgggacagca
      841 gccccagtac atcgaaaatc agttgcgggc cttcatcgag cacaggcgaa aaaacctgat
      901 catggccaat gtggcgcatg ctctgtcgcc gtcgatgctg tcggcgttgg ctcatcattt
      961 caagggtctc aatccacccc cgctgggcgg cggaccgagg ggagacgtga ccacgggaaa
     1021 acgcatttac gaagagggag tccccgaatc taatgtcccc gcatgctccg catgccatgg
     1081 tacggacgcc cacggtcagg accagattcc gcgcttggcc ggccagcttc ctgactactt
     1141 ctacagcaag ttggtgaact ggacctcgga gcgcggtcag aacacctcaa tgccggattc
     1201 atcggccgtc atgggcccga cctcgcacaa cttgaccaaa tcccagatct cagccgtcgc
     1261 tgcctatgtc agtaatctca ggtagttttc accatttgac cggtcgccgg tttcgaggaa
     1321 tgcagaaagt gcggaagctc agatcatgaa gcgcatcggg ctgaaactaa cgatcctggc
     1381 catcctcgtt tcctcatggg cgggaggctc gaaggcggcc agcattcgca attgcacctg
     1441 gtgccacgga actggggcgc agggctatgc ggccgcgccg cgactggccg gacagcggtc
     1501 catgtatatc gaggcacagc ttcgcggcta tgccgcgcat atccgcgaca atccgttttc
     1561 gaaacaatac atgtggggag cggtggcgaa tcttcgtagc gacgcggtcc gggatctggc
     1621 cgattatttt gcatcattgc cgccgaaagc cgcccatgat ggccgtcggg cgctcgtaga
     1681 caaggggcaa acgatttacg atcaaggaat tccggaggcc aacattgtct cctgtcttgc
     1741 ttgtcatggt ccgggtggcc agggcgtgcg agagatcccg cgtctcggag gattgtccta
     1801 tttctacctg aagcggcggc tcgaagaatg gggccgagga tatcattcgg ccgcatatcc
     1861 aatgcctacg gttgcgagca cgttgggccc ggacgaaatc gaagctctcg cttcgtacct
     1921 gagctttgtc aagtaagcct gccgtcgtcg cgctgttcag ccaacgctgc atcttcgcta
     1981 aacgcgcccc gggctccagc caggacatcg tccgcgaaaa aaaacggcgg gccttgcggc
     2041 gcgccgactt cctgaattct tggtaacttc gatcgtggct tcgcttttgt ttgttgcaat
     2101 caagcgcaaa cagcccctcc atagacgcag tctgtctgcg ccaaacgacc gcccaattca
     2161 tcaatcgcgc ggcatggacg cgtccgtttg actgaggtca atacggtcga tgttttccgc
     2221 tttaccattg ccaaggcgcg gtatcgcagg gagcggtcat gaagaagata tgccttaccg
     2281 tcttgatggt ctgcgcctgg ggcaatgcca gttacgccct cgatcgcgag cagcagcacg
     2341 gaaaggaact gctgggaacg ctatgcggac ggtgccatgc cgtcggccag accggccgga
     2401 gcccgcacgt cgatgccccg ccgtttcgca cgctggggga cagcaagctt tacgacaatg
     2461 actttgcggc acggttggag gaagggctga gcaccattca cccggatatg ccgactttcc
     2521 ggttcagccg ggaggacgcc gaggcttcgg tcaactacct caagtccatc caggggcaca
     2581 aacaacccaa gtagcgtcgg cccatcaaaa agccggcggt ctcgcgacgc gccggcttca
     2641 cgattggcat tgtcagccgc tcacaccttg accagcggac ctcgcgaggc cgggcccttc
     2701 gagccgccgc ccggacctcc cgacttcggc ttgcgcggcg cg
//
LOCUS       DATMGX010000740         2692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_245374, whole genome shotgun sequence.
ACCESSION   DATMGX010000740 DATMGX010000000
VERSION     DATMGX010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245374"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VNR65_12185"
     CDS             <1..251
                     /locus_tag="VNR65_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19473.1"
                     /translation="AIVAPKIVSGFACNAEVLPPGEALRALAPSTLFQSPGDRMQSFA
                     RMSALIRNIPCWRLYVGGDPSAAQPLLEAIIAGRSPNP"
     gene            248..1012
                     /locus_tag="VNR65_12190"
     CDS             248..1012
                     /locus_tag="VNR65_12190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWJ19474.1"
                     /translation="MTVKDLVSIIIPAYNAEAFIKDAIESVLAQDYRPIEVIVVDDGS
                     SDETACIAERFGPPVICHRQKNGGPPVARNRGLLIARGDYIGFLDADDLYEPGRMKLQ
                     FDKLQQNPGLAIVMGRLMREQLDSEPGGPIAFKPLVSDDYICLQLGLCLFRRTVFERV
                     GVMDESLRHSDDWDWFMRARELQVPMLLHEDLVLRARLHRNNMTRDRKAGMHFLPIML
                     KRSIDRRRTIAKGQPQSLTKLAVHRETAKPKDGGGK"
     gene            1009..1734
                     /locus_tag="VNR65_12195"
     CDS             1009..1734
                     /locus_tag="VNR65_12195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ19475.1"
                     /translation="MIRNVTPSMVSVIVPTYNAEAYLAETMASVLEQNWEPLELLVVD
                     DGSSDRTVAVAKGFGDLARVIACSHRGLAATRNSGVAAARGEFLLHLDADDLLTPKSI
                     FTRMQHFSADPTFDMVVGKLMCFLSPELSDKERTRYAAPPEPQQGHLPGAAIIRAAAF
                     DKYGRIDERFAVNADLDWSVRARDEGARIRLIDDVVVKRRIHGKNLTLTSKQELDASR
                     LHIVRASLARRRRGAQGGQGGDA"
     gene            1731..>2692
                     /locus_tag="VNR65_12200"
     CDS             1731..>2692
                     /locus_tag="VNR65_12200"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWJ19476.1"
                     /translation="MSLLAVLDESEQFLTLAQKRMLDAVLGEGAVSDAAIRDWAAAVD
                     INALDAASYRLVPALCARAGSNPALRPLYGRMKGIYRYFFYRNNRFLTFVERVFSALV
                     AAGIDFIVFKGTSTLLQYYRSAALRSFGDCDILVQRRDKERAEEVLVTCGLSYRYDAE
                     RKLRDRHSYDFVDAAENGFDLHWYSLLESCEEGIDDGLWNRSHHIEWKSLRLRVLAPE
                     DELLVAGMGWIREPMNARVDWIYDAWLILKATPDFDWRLLHEELKRRKLQLSFMSAIG
                     LLHRFVPHFPNVAVEKELFHEIRSVVERLVAENRTFGLDPETDR"
BASE COUNT          547 a          772 c          795 g          578 t
ORIGIN      
        1 gagctatcgt ggcgccaaaa atcgtgtcgg gctttgcctg caacgctgaa gtcctcccac
       61 ccggcgaagc gctgcgggcc ttggctccga gcacactctt ccagtcgcca ggggaccgga
      121 tgcaatcctt cgcgcggatg agcgcgctca tccgcaacat accttgctgg cgtctatacg
      181 tcggcggcga ccccagtgcc gcgcaaccgc ttcttgaagc catcattgca ggaaggtctc
      241 cgaatccatg acggtcaagg atttggtcag catcattatt cccgcctaca atgccgaggc
      301 tttcatcaag gacgccatcg agagcgtgct ggcacaggac taccgtccga tcgaggtgat
      361 cgtggtagat gacggctcaa gcgacgagac ggcgtgcatt gccgaaaggt tcggcccgcc
      421 tgtgatctgc catcggcaga agaacggcgg gccgccggtt gcgcgcaatc gtggccttct
      481 aatagcgcgc ggcgactata ttgggtttct ggacgccgac gatctgtatg agccaggtcg
      541 catgaaactt cagttcgata agctccagca aaatcccggc ctagccatcg tgatgggacg
      601 cttgatgcgc gaacaactcg acagcgaacc gggcggaccg atcgctttca aacccttggt
      661 gtcagacgac tatatatgcc tccaattggg gctctgcctc ttccgccgca ccgtgttcga
      721 gcgagtcggc gtgatggatg agagcctccg gcattctgac gactgggact ggtttatgcg
      781 cgcgcgtgaa ttgcaagtgc caatgctttt gcatgaagat ctcgtgctgc gcgcccgatt
      841 gcaccggaat aacatgacgc gcgaccggaa agcaggcatg catttcctgc cgatcatgct
      901 caaacgttcc atcgatcgtc gtcgtaccat agcgaagggg caaccccagt ccctgacaaa
      961 gctcgccgtg catcgtgaaa ccgccaagcc gaaagatggt ggcggaaaat gatacgaaat
     1021 gtcacaccga gcatggtgag cgtcatcgtt cctacctaca atgccgaagc ctaccttgcc
     1081 gagacaatgg cgagcgtgct ggagcagaac tgggaaccgc tcgaactact cgtcgtggac
     1141 gacggctctt ccgaccgcac cgtcgccgtt gccaaaggct tcggcgacct cgcaagagtg
     1201 atcgcatgtt cgcatcgcgg ccttgccgct acccgcaaca gcggcgtagc ggctgcgcgc
     1261 ggcgagttct tactgcatct ggatgccgat gatctgctga caccaaagtc catcttcacc
     1321 cgcatgcagc atttctcagc cgatccgacg ttcgacatgg tggtgggcaa gctgatgtgt
     1381 tttctgagcc cggaactgtc ggacaaagag cgtacccgct acgcggcgcc gcctgaacca
     1441 cagcaaggac atttgccggg cgcagccatc atccgcgccg ctgcatttga caagtatggc
     1501 cgcattgatg agcgctttgc cgtcaatgcc gatcttgatt ggtcggtgag agcgcgggat
     1561 gaaggtgcgc gcatccgcct tatcgacgac gtggttgtca aacgccgtat ccatgggaag
     1621 aaccttacgc tcaccagcaa acaggagctc gacgcgagcc gcctgcatat cgtgcgcgcc
     1681 tcgctcgcac gccgccgaag aggcgcgcag gggggtcaag ggggcgatgc atgagtcttc
     1741 tcgccgttct tgacgagagc gaacagtttc tcaccctggc tcagaagcga atgttagatg
     1801 ccgtgttggg cgaaggcgct gtgagcgacg cagcgatcag ggactgggcc gctgcggtgg
     1861 atatcaatgc tctcgatgcc gcttcgtacc gcctcgttcc ggcgctctgc gcgcgcgccg
     1921 gttccaaccc agcgcttcgg ccgctttatg ggcggatgaa aggcatctac cgttactttt
     1981 tctaccgtaa caatcgcttt ctcacctttg tcgaacgcgt cttctcggcg ttggtggcgg
     2041 ctggaatcga cttcatcgtg ttcaagggga cttcaaccct gctgcaatac tatcgcagcg
     2101 ctgcgcttcg gtcgttcggc gattgtgata ttctcgttca acggcgcgac aaagagcgcg
     2161 ccgaagaggt gctggtcact tgcgggctct cttatcgcta tgacgctgag cgtaagctta
     2221 gagataggca ttcctatgac ttcgttgacg cggccgaaaa tggtttcgac ttgcattggt
     2281 actccttgct ggaaagctgc gaggaaggaa tcgacgacgg gttatggaac cgttcgcacc
     2341 acattgaatg gaagagcctg cggctccgcg tcctagcacc ggaagatgaa ttgcttgtgg
     2401 ctgggatggg ctggattcga gagcccatga acgcgcgtgt cgattggatt tacgatgctt
     2461 ggctgattct caaggcgacg cccgatttcg attggcgtct gcttcatgag gaattgaagc
     2521 gtcgaaagct tcagttatcc ttcatgagcg cgattggttt gctgcatcga ttcgtaccgc
     2581 attttccgaa cgtcgcggtg gagaaggaat tgttccacga gatacgaagc gtcgttgagc
     2641 gactagttgc ggagaaccgt acctttggcc tcgatccaga gaccgaccgc ca
//
LOCUS       DATMGX010000741         6603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_257804, whole genome shotgun sequence.
ACCESSION   DATMGX010000741 DATMGX010000000
VERSION     DATMGX010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257804"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VNR65_12205"
     CDS             complement(<1..484)
                     /locus_tag="VNR65_12205"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HWJ19477.1"
                     /translation="MNIRSMTGYSQVKMNADDQLTFTLSLKAVNHRFLDLHLRLPPEM
                     DPVEMKIRRVLKERLHRGHIEVTLSLERSGGVAFSVNRDLVGGYLRTFREVAEEFGVS
                     AEPDLNAVLKMPGALNAAENGDAMAALEKHVTEALEQCIERLNHMREEEGRGAAVELR
                     E"
     gene            complement(492..2327)
                     /locus_tag="VNR65_12210"
     CDS             complement(492..2327)
                     /locus_tag="VNR65_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ19478.1"
                     /translation="MRQLSRLLRYFKPYWLHMLAAVVSMALVGLLDAFRLLLIGPIFD
                     LVLNPSSKSHSLPLFKMPISHKPIELNWFVPEHFHNVWTMVAFALVASTALKGIFDYG
                     GSYLANYAGYGMVTDLRNELYSATLRRSVAFFQKYSTGTLLSAIVNDIERVQFALSSV
                     LAEFLQQFFTFLFTACVVVLLGGRLTWVLIIFVPVVVMAARRIGHRVRTTTRRGQDKL
                     AEIQNILQETITGVRIVKAFSMEAWETRRFRDAGRRLFKANLRSVSAYAVSSPMMDIF
                     GAIAIAILILIGREYIKHGFFTEGVFLAFIIAVFKLYEPVRKFGIFNNNFQQALGASS
                     AIFDFLDAQDEVREKPGAQKLPPFHQGIRFEHVDFSYQQEDDDREVLHDINLEVAPGE
                     LVAIVGSSGAGKTTLVHLLPRFFDVTRGRLLIDGRDVRDLTVASLRSQIAIVTQDTIL
                     FNDTVRNNIAYGQPDVSAETVEEAARAALAHEFIMALPEGYDSVIGERGLRLSGGERQ
                     RISIARAILKNAPILVLDEATSALDTESESLVQVALQNLITGRTVFVIAHRLSTVRRA
                     DRILVVENGMIADEGTHECLLTHPGTYRRLYDLQFVDVETTTGTE"
     gene            complement(2479..3384)
                     /gene="rapZ"
                     /locus_tag="VNR65_12215"
     CDS             complement(2479..3384)
                     /gene="rapZ"
                     /locus_tag="VNR65_12215"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWJ19479.1"
                     /translation="MAGLKKKSIRAPKSRDQHELVIISGMSGAGKASALKTFEDLGYY
                     CVDNLPVGLISNFAELVLDSLEIQRAALVVDIREGARLDKLPQVLGSLRRMLKTTVVF
                     LEADTDVLVRRYSETRRPHPLGRDASVRSSLTAERRRLQPLRALADLVVDTTRFNVHE
                     LRGQLTERFQGGNRDKNLLISCVSFGYREGVPEDADLVFDVRFLPNPHFIPKFRPLTG
                     RHPEVAKYIRSFPQTREFINRISELLIYLIPHYIREGKSYLTIAFGCTGGKHRSVMIA
                     EDVKKRLAKAGYNVKVVHRDSPAAS"
     gene            3608..6031
                     /locus_tag="VNR65_12220"
     CDS             3608..6031
                     /locus_tag="VNR65_12220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HWJ19480.1"
                     /translation="MTLRLSMNLRTLPLLLLACLGIFSAEPAIADRIPQAIDDTSVWR
                     LRGSVPPIARPEFDRGKVNAALAMQGMKLVFRLTPAQQSSLDTLLRQQLDPASANYHR
                     WLTPEQYADRFGLSVTDIKRAGTWLRQQGFTNVTPARSRTWIAFSGRAGQVESAFRTP
                     IHQYVVNGNTHYANTGEPSLPGAFRGVVLGIGALNDFRPKPRGIVKSVHPNFTSEISG
                     KHFLAPDDFATIYDIQGLYGSGTTGAGQTIAVVGQSDLSTDTNHNNQYDVLTFRSVSN
                     LPTVTLQVVLVPADKDPGIVSGDVDEANLDLEWAGAVAKDASLIYVNSQNALFNAMQY
                     AVDQNLAPVISVSYGLCEAQFSSTEIGTLTALTQQANAQGQTIVASSGDSGPADCDFS
                     TDPNNPVKSATHGYAVDVPASLPNVTGMGGTEFSEGDTLGATQYWSGSNNGNNGSALS
                     YIPEMVWNDTVTDGSLSASGGGASKLFSKPSWQTGAGVPADGQRDVPDLSLSSSADHD
                     GYLICSQSSCVTGYRKSDQTLTVIGGTSVAAPTFAGIVALIVQKTNDRQGNVNQYLYS
                     LAASSPNVFHDITTGDNMVPCTAGSTDCPASGMIGYSAGPGYDLTTGLGSVDAAALVA
                     AWNGPTNPDFQISAQSLSLTVTRGTPVTDTLTVTGLAGFSQTVNLSCVVSSTLTGTTC
                     SVSPSSVAPGGTATLTVTASPLAGMLRVNPLFLHPGGWMGTGFAFAAGLLLTRAKPTR
                     TRRKQSGRWSAMLMLLLIGILTLTTSCGGGSSSQQASPPPAPQSGTVTVQASSGDLNH
                     NLSIAVTVN"
     gene            complement(6050..>6603)
                     /locus_tag="VNR65_12225"
     CDS             complement(6050..>6603)
                     /locus_tag="VNR65_12225"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWJ19481.1"
                     /translation="RLLAEAVKKANDAILDYAEEHKNTTGMGTTLVAARFSDGVFSIA
                     NVGDSRIYLFREGQLLQLTEDHSLVMEQVRRGMLTLEEAKRSSAQNIITRALGTDEST
                     LPDLGEFPAQEGDILLLTTDGVLRHVADQEMRNILLQLPSLEAACQTLIDAANEGGGE
                     DNATCVLVRVRKGKPIDDQKNES"
BASE COUNT         1338 a         2027 c         1943 g         1295 t
ORIGIN      
        1 gttcgcgcaa ctcgaccgct gcgccacggc cctcttcctc gcgcatgtgg ttcagccgct
       61 cgatgcactg ctccagcgcc tccgtcacgt gcttctccag cgcggccatg gcatcgccgt
      121 tctcggcggc attcagcgcg cccggcattt tgagcaccgc gttcaggtcg ggctccgcgc
      181 tcacgccaaa ctcttcggcg acttcgcgga aggtgcggag gtatccgcct accaggtctc
      241 ggttcacgga aaacgccacc ccgccgctgc gctccaagct cagcgtcact tcgatgtgtc
      301 cgcggtgcag gcgctccttc agcacccggc ggatcttcat ctcgaccggg tccatctccg
      361 gaggaaggcg cagatgcaga tcgagaaaac gatggttcac tgccttcagg ctgagggtga
      421 acgtgagttg atcgtccgcg ttcatcttca cttgcgagta gccggtcatg gaacggatgt
      481 tcatagtgga gtcattcagt tccggtggtc gtctctacat cgacgaactg caaatcatac
      541 aaccggcggt aggtgccggg gtgagtcagt aaacactcgt gtgtgccctc gtcggcgatc
      601 attccgtttt ccaccaccaa aatacggtcg gcgcggcgca ccgtggacaa ccggtgcgcg
      661 atcacaaaga cggtgcgccc ggtaatcagg ttttgcaggg ccacttgtac cagagattcg
      721 ctctcggtgt cgagcgcgga agtggcttcg tcgaggacca ggatgggcgc gtttttcaga
      781 atggcgcgcg caatcgagat gcgctgccgt tccccaccgc tcaggcgcag cccgcgttcg
      841 ccgatcaccg agtcgtatcc ctcgggcagg gccatgatga attcgtgggc cagcgccgcg
      901 cgggccgctt cttccacggt ctcggcggaa acgtcaggct gtccataggc aatgttattg
      961 cgcaccgtat cgttgaacag gatagtgtcc tgggtgacga tggcaatttg cgagcgcagg
     1021 gaagcgacgg tcaagtcgcg cacgtcgcgc ccgtcgatga gcagccgccc gcgagtgaca
     1081 tcgaagaaac gcggcagcag atgcaccaac gtagtcttgc ctgcaccgct ggagccgaca
     1141 atcgccacca gctcgcccgg cgctacctcc aggttgatgt cgtgcagcac ttcgcggtcg
     1201 tcgtcctcct gctgatagct gaagtccaca tgctcgaagc gtatgccctg atgaaatggc
     1261 ggcagcttct gcgcgcccgg tttctcgcgc acttcgtcct gggcgtcgag gaaatcgaaa
     1321 atcgccgagg aagcgcccag cgcctgctga aagttgttgt tgaatatccc aaacttgcgc
     1381 accggttcgt atagtttgaa caccgcgatg atgaatgcca ggaagacgcc ttcggtgaaa
     1441 aagccgtgtt taatgtactc gcgtccaatc aggatcagga tggcgatagc gatggcgccg
     1501 aaaatatcca tcatcggcga gctcacagca taggcgctca cggagcgcag gttggctttg
     1561 aacagccgcc gcccagcatc gcggaagcgg cgcgtctccc aggcttccat cgaaaaggct
     1621 ttcacgatgc gcacgcccgt gatggtttcc tgcaggatgt tctggatctc agccagcttg
     1681 tcctggccgc ggcgggtcgt agtgcgcacg cggtggccga tccttctggc cgccatcacc
     1741 accaccggaa cgaagatgat gagcacccac gtcaaacgtc cccccagcag gaccaccacg
     1801 caggccgtaa agaggaaggt aaaaaactgc tgcagaaatt ccgccagcac cgacgagagc
     1861 gcaaactgca cccgctcgat atcgttcacg atcgccgaca gcagtgtgcc cgtggaatat
     1921 ttctggaaga acgccaccga acggcgcagc gtggccgaat agagttcatt acgcaaatcc
     1981 gtaaccatgc cgtacccggc atagttggcc aggtagctgc cgccgtaatc gaagatgccc
     2041 ttcagcgccg tggaggcgac cagcgcaaac gcgaccatcg tccacacatt gtggaaatgc
     2101 tcgggaacaa accagttcag ctcgattggc ttatggctga ttggcatttt gaacaagggg
     2161 aggctgtgcg atttggatga aggattcagt accagatcga agatgggccc gatcaacagc
     2221 aaccggaagg cgtcaagtag ccccaccagc gccatggaca ccacggcggc cagcatgtgg
     2281 agccaatacg gtttgaagta gcgaagaagg cggctcagtt gacgcatagt tcccgcaggt
     2341 tcgattgctg acttagctcc gcgaagaaat aggccttggg agaatcggga tgcaacgtta
     2401 attctactgg ataacgcggc taccacgaag cattaccccg cgcccgggtg taacacagac
     2461 tcagcagcgc tctgcttatt agctcgccgc cggggagtcg cgatgcacca ccttcacgtt
     2521 gtacccggcc ttggccaaac gcttcttcac gtcttcggcg atcattaccg agcgatgctt
     2581 gcctcccgtg catccgaagg cgatggtcag gtagctcttc ccttcccgga tgtaatgcgg
     2641 gatcagatag atcagcagct cggagatacg attgatgaac tccctggttt gcggaaacga
     2701 gcgaatgtac ttggccactt cgggatgccg cccggtcagg ggacgaaact tggggatgaa
     2761 gtgcggattg ggcaggaagc gcacgtcgaa caccaggtcc gcatcttccg gaacgccttc
     2821 gcggtagcca aagctcacgc aggaaatcag caggttcttg tcgcgattgc cgccctggaa
     2881 gcgctcggtg agctggccgc gcaattcgtg aacattgaac ctggttgtgt ccaccaccag
     2941 gtcggccagt gcgcgcagcg gttgcagccg gcggcgttca gccgtgagcg acgaccttac
     3001 ggaggcgtcg cgcccgagcg ggtgagggcg ccgcgtctcg ctataacgcc gaaccagcac
     3061 atcggtatcg gcttccagaa agaccaccgt ggtcttgagc attcgccgca gggagccgag
     3121 aacctgcggc aacttgtcga ggcgcgcgcc ttcgcggatg tccaccacca gcgcggcgcg
     3181 ctggatctcc agcgaatcca gcaccagttc ggcaaaattg gagatcagcc caaccggcag
     3241 gttgtcaacg cagtagtagc cgaggtcctc gaacgttttc agggccgagg ctttcccggc
     3301 tccgctcatg ccagagatga tgaccagctc gtgctggtcg cggcttttag gcgcgcgaat
     3361 cgatttcttc ttcaggccag ccaccttccc tcagacccat ctcgtgttgg gtttggcaga
     3421 cggcgtcgct cgtagctgcc tcattggggc gagagtacca gagttgctcc ccggcgtcag
     3481 ccgaaacctt cgtcaccaaa tggatgcaac aaccgtgggt gaagtctcac cttcatcgga
     3541 catcctggtc gcgtccgcgt ttcgcttcgg ttaatatgca gtcagcgcga tagcgtccgg
     3601 aaaacccatg actctccgtt tgtcgatgaa cctacgcacc ctgccactgc tcctgttggc
     3661 atgcctcggc atcttctcgg cggaaccggc tatcgccgac cggattccgc aggcgatcga
     3721 cgacaccagc gtgtggcgcc tgcggggcag cgttccaccg atcgcgcgcc ccgagttcga
     3781 tcgcgggaag gtgaatgctg cgctagccat gcaaggcatg aagctggtct tcaggctgac
     3841 gccggcgcag cagagcagcc tggacacact acttcgacaa caactggatc ctgcgtccgc
     3901 gaactatcac aggtggctca cccccgagca gtatgcagac cgcttcggcc tgagcgtcac
     3961 cgatattaag cgggccggga cgtggttacg ccagcaaggc ttcaccaatg tcacgccagc
     4021 gcgcagccgt acctggatcg ctttcagcgg ccgggccggt caagtggaat cggctttccg
     4081 cactccgatt catcagtatg tcgtcaacgg caacacgcac tatgccaaca cgggcgagcc
     4141 gtcgctgccc ggcgcattcc gcggagtcgt tttggggatc ggcgctctca acgattttcg
     4201 tcccaagccg cgaggcatcg tcaagagcgt gcatcccaat ttcacctcgg agatctccgg
     4261 aaagcacttc cttgcgcccg acgacttcgc caccatctac gacatccagg gactgtatgg
     4321 cagcggaacc accggcgcgg gccagaccat cgccgtagtc ggtcagtcgg atttgtccac
     4381 ggataccaac cacaacaatc aatacgacgt gctgacgttt cgtagtgttt ccaatctgcc
     4441 gacagtaacc ctgcaagtgg tgctggttcc cgccgacaaa gatcccggaa tcgtcagcgg
     4501 cgatgtggac gaggccaacc tggatctgga atgggcaggc gcagtcgcga aagacgccag
     4561 cctgatctat gtgaattcgc agaatgccct gttcaacgcg atgcagtacg cggttgacca
     4621 gaacctcgcg ccggtcatca gcgtcagcta cggactttgc gaagcgcagt tcagcagtac
     4681 cgagatcggc accctgactg cgctgaccca gcaggccaat gctcaaggac agaccatcgt
     4741 tgcttcgtcc ggagatagcg gccctgccga ttgcgatttc agtaccgatc cgaataaccc
     4801 ggtaaagagc gccacccatg gctatgccgt ggatgtacca gccagccttc cgaatgttac
     4861 cgggatgggc ggcactgaat tcagcgaagg ggatacctta ggagcaacgc aatactggag
     4921 cggcagcaat aacggcaata atggctcagc cctttcctac attccggaga tggtctggaa
     4981 cgataccgtg acggatggct ccttgtcggc gagtggcgga ggagccagca agctcttcag
     5041 caaaccatcg tggcagactg gcgcgggtgt gccggccgat ggccagcgcg acgtacccga
     5101 tctttcgctc agttcttcgg ccgaccacga tgggtatctc atctgctcgc aaagcagttg
     5161 cgtaaccggg tacaggaaat ccgatcagac gttgaccgta atcggaggga cgtctgtcgc
     5221 ggccccgacc tttgccggca tcgtggcact gatcgtgcaa aagaccaatg atcgccaagg
     5281 taacgtcaat caataccttt actcgttggc ggccagctca cccaacgttt tccacgacat
     5341 caccaccggc gacaacatgg tgccctgcac ggcgggttca acggattgcc ctgccagcgg
     5401 catgattggc tatagcgcgg gaccaggtta cgacctgaca accgggttgg gatcggtgga
     5461 cgccgctgcg ctggtggctg cgtggaatgg gcctacgaat ccggacttcc agatttcggc
     5521 gcagagcctg agtcttaccg tcacccgagg aactccggta accgacacgc tcaccgtaac
     5581 cggactggcc gggttctccc agaccgtgaa cctctcgtgc gtcgtgtcgt ccacgctgac
     5641 cgggaccact tgctcggtga gtccgagcag cgtcgctcct ggcggaacgg caacgctaac
     5701 tgtaaccgcc agtccgctag ccggaatgct gcgggttaat ccacttttcc tgcatcccgg
     5761 cggttggatg ggaacaggct tcgcatttgc cgccggtctt ctgcttacgc gcgctaaacc
     5821 cacacgcacg cggcgcaagc aaagcggccg gtggagtgcg atgctcatgc tgctgctgat
     5881 cggaatactg acgctcacga cttcctgcgg tggaggcagc agcagccaac aagccagccc
     5941 ccctccggct ccccagagtg gcacggtgac ggtgcaggcg agcagcggcg acctcaatca
     6001 caacctatcg atcgcagtga cggtgaatta gtgcgattcc aggactgccc tagctttcgt
     6061 tcttttggtc gtcgatcggt ttgccttttc gcacgcggac aagcacgcaa gttgcgttgt
     6121 cttccccacc accttcatta gctgcatcga tcagggtttg gcaggccgcc tccagcgatg
     6181 ggagttgcag cagaatgttg cgcatctcct gatcggcgac gtggcgcagt acgccgtcgg
     6241 tggtcagcag caggatgtct ccctcctgcg ccggaaactc gccaagatcg ggaagcgtac
     6301 tttcatcggt gccgagggcg cgggtaatga tgttctgcgc cgacgagcgc ttggcttctt
     6361 ccagggtgag catcccgcgg cgtacctgct ccatcaccag ggagtggtcc tcggtcaact
     6421 ggagtaactg tccttcgcga aacaggtaga tgcggctgtc gcccacattg gcaatactga
     6481 aaacgccatc cgagaagcgc gccgccacca gggttgtccc catccctgtc gtgttcttat
     6541 gctcttcggc ataatccaga attgcatcgt tggccttctt gacggcttcg gcgagcagac
     6601 gtg
//
LOCUS       DATMGX010000742         3650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_368188, whole genome shotgun sequence.
ACCESSION   DATMGX010000742 DATMGX010000000
VERSION     DATMGX010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368188"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /gene="nuoF"
                     /locus_tag="VNR65_12230"
     CDS             complement(<1..891)
                     /gene="nuoF"
                     /locus_tag="VNR65_12230"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWJ19482.1"
                     /translation="MLSDKDRIFKNLYGQYDWRLNGARERGAWNSTKGLIQRGQDWIV
                     DEVKASGLRGRGGAGFSTGLKWSFMPKKSDGRPNYLVVNADESEPGTCKDREILRHDP
                     HLLVEGCLLASFAMRANTCYIYIRGEFIHERQRLQAAVDEAYAARLIGKNNVHGWDLD
                     IYIHHGAGAYICGEETALLESLEGKKGQPRLKPPFPANAGLWGAPTTVNNVESIAVVP
                     DILRRGGEWFAKLGKPNNTGTKLFCISGHVNEPCNVEEEMGITLRELIDTHAGGVRGG
                     WDNLLAVIPGGSSVPCVPAHM"
     gene            complement(895..1572)
                     /gene="nuoE"
                     /locus_tag="VNR65_12235"
     CDS             complement(895..1572)
                     /gene="nuoE"
                     /locus_tag="VNR65_12235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005724.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HWJ19483.1"
                     /translation="MSVRRLDPNQPSDFEFTPENLAWAMEQIKKYPEGRQQAAVIPIL
                     WRAQEQHDRWLPEPAIRYVADLLEMPYIRVYEIATFYTMFNLAPVGRYHVQLCGTTPC
                     MLRGANEIKAVCEREIGPQGQVTREGVFSWMEVECLGACVNAPMAQINDDYYEDLTPE
                     NFKALLDGLRKGRDMKPGPQTARQFSAPVSGPTTLKNGALIEADSPGGAAPDAAGTRQ
                     GKTKDED"
     gene            complement(1678..2136)
                     /locus_tag="VNR65_12240"
     CDS             complement(1678..2136)
                     /locus_tag="VNR65_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19484.1"
                     /translation="MVAFNNEISSAEKIGLHLSEIGRLQNNAGSLLQFTAILFAICIY
                     FYNDMLYPSTDNRVIYASLLAVTASFILAACFLYLRCLTLSVGWSAKVKHPKTKFHLL
                     STLSEEEIEETLYLIVRSFRRGTYCFFLSFFSSFVLLGMVALGQLKMPSQ"
     gene            complement(2557..3468)
                     /locus_tag="VNR65_12245"
     CDS             complement(2557..3468)
                     /locus_tag="VNR65_12245"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19485.1"
                     /translation="MLLDIRQRLEYGLVLAISLLMRAMPLKMATAFSASAWRFIAPRT
                     RRHRRALKNLQKAMPEKSSAEHEAIALGMWDNLGRVMAEIFQLDRLLKDPSRFEFENP
                     ALISRYRDKTGPLVSAALHTGNWEVAILPLHAINARPAGVYRIVENPYVDRWLRNKRS
                     ALYPSGLFATKSDEGLATVMRISAHLRAGGALGMLADLVDWKGIQVPFFGQMMWATVA
                     PAWLARRAGTRLCVGRCIRIGKESRFKIAFKELKVPRTDKADEDIRAMTAAIQKQFEA
                     WIREHPEQWMWSNKRWPDNELPGGTQD"
BASE COUNT          762 a         1079 c         1064 g          745 t
ORIGIN      
        1 catatgcgcc ggcacgcaag gcacggaaga cccgcctgga atcaccgcga gaaggttatc
       61 ccagccgccc cggacgccgc ccgcgtgcgt atcgatcagc tcgcgcagag tgatgcccat
      121 ctcctcttcg acattacaag gctcattcac atgccccgaa atgcaaaaga gcttcgttcc
      181 cgtattgtta ggcttgccca gcttcgcgaa ccattcaccg ccgcgcctca ggatgtcagg
      241 tacgacggca atggattcga cattgttgac ggtcgtcggc gcgccccaca gccccgcatt
      301 tgccgggaac ggaggcttca gccgcggctg ccccttcttg ccttccaggc tttcgagaag
      361 cgccgtttcc tctccgcata tgtacgcgcc cgcgccgtgg tggatgtaga tatccaagtc
      421 ccaaccgtgg acattgttct tgccgatcag gcgcgcggcg tatgcctcat cgacagcggc
      481 ctgcaagcgc tggcgctcgt ggatgaattc gccccggata tagatgtagc aggtgttggc
      541 ccgcatcgcg aaagaggcga gcaggcagcc ttcgacgagc aggtggggat cgtgccggag
      601 gatctcccgg tccttgcagg tgccgggctc ggattcatcg gcgttgacca cgaggtaatt
      661 cgggcggccg tcagactttt tcggcatgaa ggaccatttc aggcccgtcg agaatcccgc
      721 gccgccgcgc cctcgaagcc cgcttgcctt cacctcatca acgatccaat cctggcccct
      781 ttgaatgagc cccttggtgc tgttccaggc tcctcgttcg cgagcgccgt tcagccgcca
      841 gtcgtactgc ccgtacaggt ttttgaaaat gcgatcttta tcggacagca tggatcaatc
      901 ctcgtccttc gtcttgccct ggcgcgttcc agccgcatcc ggagcggccc cgcccggcga
      961 gtccgcctcg atcagcgcgc cattcttaag tgttgtcgga ccgctgacgg gagcggagaa
     1021 ctggcgggcg gtctgcggcc caggcttcat gtcgcgccct ttccgcaaac cgtcgagcag
     1081 cgctttgaag ttctcgggcg tcagatcctc gtaatagtcg tcgttaatct gagccatggg
     1141 cgcgttcacg caagcgccaa ggcactcaac ctccatccac gagaagacgc cctcgcgtgt
     1201 gacctgcccc tgcggcccga tctcccgctc gcaaacggct ttgatctcat tcgcgccacg
     1261 gagcatgcaa ggcgtcgtcc cgcaaagctg cacatggtat cggccgacgg gcgccagatt
     1321 gaacatggtg tagaaggtcg cgatctcata gacgcggatg tatggcatct caagaagatc
     1381 ggcgacgtag cggatcgcag gctccggcag ccagcgatca tgctgttcct gcgctctcca
     1441 aaggatcggt atgaccgcag cctgctgacg cccttccggg tactttttga tctgctccat
     1501 ggcccacgca aggttctcag gcgtgaactc gaagtcgctc ggctggttgg ggtcaagacg
     1561 gcggacggac atgcggcgtt tccatgggtt cttgtttgtt aaataaccga gataccggac
     1621 aagagctaga ggttaagagg gcgcagcgaa gacacggggg ttggcatctc cgcgctgcta
     1681 ttgagaaggc attttcagct gacccaaagc caccatgccg agcaagacaa aggagctgaa
     1741 gaaacttagg aagaagcagt aggtgccgcg ccggaaagag cgcacgatga gatagagcgt
     1801 ctcctcaatc tcttcctcgg acagcgtaga gagcaggtga aacttcgtct tggggtgctt
     1861 gaccttcgcg ctccaaccga cggaaagcgt caagcatcgg agatatagga agcatgccgc
     1921 gagaatgaag gacgctgtca ccgcgagcaa ggaggcataa atcacccggt tgtcggtcga
     1981 aggatagagc atatcgttgt agaagtagat gcagatggca aagaggatcg ccgtgaattg
     2041 caggagggaa ccggcattgt tctgcaggcg gccgatctcg gacaaatgca gaccgatctt
     2101 ctcggcggac gaaatctcat tgttgaaggc gaccatctct cccccgacgc gcccagctga
     2161 tcaagcgccg ctttcgcttg tcacataccg gggaaagtcc ggcccggcaa ggctgactaa
     2221 gccgccgtca acgcgaatgc caaccgtaaa accgttcgcc ctatcatcaa atggagcata
     2281 tccaaagcgt cggcgtaatc ctggtctgat acagatggtt aacttcattg ggtttgtttc
     2341 gtaaaagtgg ccataagtaa atatgtaagc aattaaacgc aaaagtttca tgccgaatac
     2401 gtatttttat aacgctcgta ttatcaattt ggaattttag gaaattcgcg ttgcttggga
     2461 agagcctcca aaccttcgcg cgcgcggggg ttgaaacgct tttttaagtg aatatgcaag
     2521 gtctgttaac cgagaaagcg ttgcgcgctt gcgcgatcag tcttgcgtcc cgcccggcaa
     2581 ctcattgtcc ggccagcgct tattagacca catccattgc tccgggtgct cgcgaatcca
     2641 ggcctcgaat tgcttttgga tggcagccgt catcgcccgg atgtcctcgt cggccttgtc
     2701 cgtgcgcggc actttcagct ccttgaaggc gattttgaag cgcgattctt tgccaatgcg
     2761 gatgcagcgg ccgacgcaaa gacgcgtgcc ggcgcggcgg gcaagccagg cgggtgcgac
     2821 cgtcgcccac atcatttgac cgaaaaacgg aacctggatg cccttccaat ccacgaggtc
     2881 ggccagcatg ccaagcgctc cgccagcgcg caaatgagcg ctgatacgca tcaccgtcgc
     2941 caggccttca tcgcttttcg tcgcgaacag gccgctcggg taaagggctg agcgcttgtt
     3001 tcggagccag cgatccacgt aggggttctc cacgatcctg tagacgccag cgggccgcgc
     3061 attgattgcg tgcaagggaa gaatggcgac ctcccaattg cccgtgtgaa gcgctgcact
     3121 gaccagaggg cctgtcttgt cccgatagcg tgagatcaag gccggatttt cgaactcgaa
     3181 gcgcgatgga tccttgagca ggcgatccag ctggaagatc tcggccataa cgcgcccgag
     3241 gttatcccac atgccgagcg caatggcctc gtgttccgca gacgatttct cgggcatcgc
     3301 cttctggaga tttttaagcg cgcgccggtg acggcgggtt ctcggggcta tgaagcgcca
     3361 tgcgctagcc gaaaaggccg tcgccatctt gagcggcatc gcccgcatga gcaggctaat
     3421 ggccagcaca aggccatatt cgagccgttg gcggatatcg agcagcataa atccaggctg
     3481 ggttagcgtg cgagcacgcc tgtcttgcga ggccccggcc ggaacgtcaa gacattaaac
     3541 aaagcccacc ggacgcgcca acgcatcgca gcagtcgtcg tcccggaaag gcggagcctt
     3601 atccgggatc gtaacagatt gaagctacta gtttttggcg atcccggctc
//
LOCUS       DATMGX010000743         2021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_257831, whole genome shotgun sequence.
ACCESSION   DATMGX010000743 DATMGX010000000
VERSION     DATMGX010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257831"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VNR65_12250"
     CDS             <1..518
                     /locus_tag="VNR65_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19486.1"
                     /translation="REALIHALNDYEGAVILISHDRHLLEATVDRLWVVRDGTVTPYD
                     GDIESYRQESLGGRGKQASSAARANGGQTADVKRTRDEERRQTAQRRSELAPLRKLLT
                     EHERRVEALQKKIAAIDFSLGDHSLYEKQPDKARSLTLERATLTKELKLAEDAWFEAS
                     FAYEEAAAADG"
     gene            1008..1439
                     /gene="bfr"
                     /locus_tag="VNR65_12255"
     CDS             1008..1439
                     /gene="bfr"
                     /locus_tag="VNR65_12255"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HWJ19487.1"
                     /translation="MKGEPKVIEYLNAALRHELTAVNQYWLHYRLLDNWGYKSLAKQW
                     RKESIEEMEHADKITVRIIFLEGFPNMQVLDPLHIGQTVKEVFDADLAAEHKARDLFE
                     KLMHDEEHHIDFLETQLDLVDKLGIQLYSQYHIGGLEGGKD"
BASE COUNT          482 a          560 c          573 g          406 t
ORIGIN      
        1 gccgggaggc gctgatccac gcgctgaatg actatgaagg cgcggtcatt ctcatcagcc
       61 acgaccgtca tttgctggaa gcgactgtgg atcgcctctg ggtcgtgcgc gacggcaccg
      121 taacgcccta cgatggcgac atcgaaagct accggcagga aagccttggc gggcgcggaa
      181 agcaggcgag ttccgccgcc agagcgaacg ggggccagac ggcggacgta aagcggacac
      241 gcgatgagga gcgccgccag accgcccagc gccgctcgga gcttgcgccg cttcgcaagc
      301 ttctcaccga acacgaacgg cgcgttgaag cgttacagaa gaaaattgcc gccatcgatt
      361 tcagtcttgg cgatcacagt ctctacgaaa agcagcctga caaagcccgt tcactcactc
      421 ttgaacgcgc aacactgacg aaggagctta agctggcaga agacgcttgg tttgaagcct
      481 cgttcgccta cgaagaagca gccgccgctg acggctgata gcttagaatt attataagaa
      541 caacaactcg aagatcttct caggtatgaa ttggtagggt agaagtgtaa gaccttagaa
      601 tcattataaa ttatgttttt cattgccttc ttgagccgaa cagctaatct gtacttctgt
      661 tcctgaatcg aaggcaggct ccggcgaatg atcatctgct cctgtagtgt gttgagcgac
      721 aagcaggttc gggccgcgct agacgctccc aaccctcctc gcacgccgtg ccaggtacac
      781 aggtgcttgg gctgcgagcc gcagtgcggg cgctgcgtcc ggtccatgcg ggagctcatt
      841 gaaagggcgc gtctggaaac tgcctccgag acgctcggtg cgaaagttgc ctgaagcctt
      901 accttgattt tctcacgaga tcctacaaga tcgccggtca tagtccccgc aggccgatcc
      961 aactcggcct cacgcgttac accttgcgct gaagaccgga gaaaaaaatg aagggcgagc
     1021 ccaaagttat cgaatatctt aacgccgcgc tgcgtcacga gctgacggcg gtcaatcaat
     1081 actggctgca ttatcgcctc cttgataatt ggggctacaa gtcgctcgcc aagcaatggc
     1141 gcaaggagtc catcgaggag atggagcacg cggacaagat caccgtgcgc atcatcttcc
     1201 tggaaggctt ccccaacatg caggtgctcg acccgctgca tatcgggcaa accgtgaagg
     1261 aagtgtttga cgccgatctc gcggccgaac acaaggcgcg cgaccttttc gagaagctca
     1321 tgcacgatga ggaacaccac atcgacttcc tcgaaacgca gctcgacctt gtggacaagc
     1381 tcggcatcca gctctactcg cagtatcaca tcggcgggct cgagggcggc aaggactgag
     1441 cagccgcctt gtggggcgcc gggcgctcca tttgtcatgg ccgggctcgg accccggcca
     1501 tccagcaaac actctgatct tatgccaacc atgactctcg ccgggccgaa caggtctgtg
     1561 cggtaaggtc agcctcggcg cggcccacag gatcggtaag ctgtgctttg gcgcgggttc
     1621 ctgaaatctg catggccatt tccccatgga ccagacaatg gtccaaaata attgtggctt
     1681 tggaccaaaa tcgagtgcgg cttcaacgaa aaccagctgc cattctggct gagcgagatc
     1741 cttgcggaac gcgctttgac gagaggttaa ttgcgataag tctttgattt gtatggaata
     1801 aattgaggga ggatgacggc gccgcgcggc tgcgacaggc acggacatgg ttaacaggca
     1861 caacggaaac atatttaacg gactttagcg cgtcccagta aagggaaaaa ttccggcatt
     1921 aagttccatt taatgggaag caattgagat aaaaatatat tgttttcaag atcttatcgg
     1981 cgtcagccaa aggcgcgcgg ctcctcgtct tgcgccgaat a
//
LOCUS       DATMGX010000744         2718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_129586, whole genome shotgun sequence.
ACCESSION   DATMGX010000744 DATMGX010000000
VERSION     DATMGX010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129586"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /gene="rlmN"
                     /locus_tag="VNR65_12260"
     CDS             <1..885
                     /gene="rlmN"
                     /locus_tag="VNR65_12260"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWJ19488.1"
                     /translation="SHQHSALSKGRGVLSGGTESIAVSGTIQSGKLETRNSKLETSSP
                     QRATICVSSQVGCAVECTFCMTALLGLKRNLSAGEIVGQILAVLNDQQVELQRDRVNL
                     VFMGQGEPFLNYDNFMQAVRLVVEAVGIPESRMTVSTSGIVPRIADFGAEPVRPKLAV
                     SLNAPNEALRTQVMPITKKWTIEKLMAALREFPLRNRERLTFEYVLLGGVNDGVQHAR
                     EMVDLIRGLRAKVNLIALNSGPEIPFSTPSEERVLAFQSVLTRAGIPAFVRRPRGRDI
                     YAACGQLKHTTELVQVTG"
     gene            1155..>2718
                     /gene="dnaK"
                     /locus_tag="VNR65_12265"
     CDS             1155..>2718
                     /gene="dnaK"
                     /locus_tag="VNR65_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWJ19489.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGGEPKVIPNEEGGRTTPSVVGFTK
                     NGERLVGQVAKRQAITNPENTVYSIKRFMGRRYDEVNEEMKMVPYKVVQSGDHVAIVA
                     QGKEYTPPQVSAMILQKLKKSAEDYLGQTVTDAVITVPAYFNDAQRQATKDAGKIAGL
                     DVKRIINEPTAAALAYGLDKKKDETIAVYDFGGGTFDISILEVGEGVIEVKSTNGDTH
                     LGGDNIDQRIVDWLIDEFKKDEGLSLRDKGNEMALQRLRDGAERAKIELSTTMETEIN
                     LPFITADASGPKHLVKKLTRSKLEEMVKDIIERSMGPCKAAIKDAGISPDKIDEVVLV
                     GGQTCMPRIQQLVKELFGKEPHKGVNPDEVVAIGAAIQGGVLGGEVKDLLLLDVTPLT
                     LSIETMGGVATNMIPRNTTIPTKKTETFSTAADNQTSVEVHVLQGERPMARDNRTLGK
                     FHLTGLPPAPRGLPQIEVTFDIDANGILNVNAKDMATGKDQKITITSSSGLSKDEVER
                     MAKDADAHAAEDK"
BASE COUNT          612 a          798 c          833 g          475 t
ORIGIN      
        1 agccaccagc attcagcact tagcaaaggc aggggtgtcc tgagcggggg aactgagtct
       61 attgcggttt caggcaccat ccagtcgggc aaactcgaaa ctcgaaactc gaaacttgaa
      121 acttcatccc cgcagcgcgc caccatctgc gtttccagcc aggtgggatg cgccgtcgag
      181 tgcaccttct gcatgaccgc gctgctggga ttgaagcgca acctcagcgc gggcgaaatc
      241 gtgggacaga ttctcgccgt actcaacgac cagcaagtcg agctacagcg tgaccgcgtc
      301 aacctggtct tcatgggaca gggtgagccc ttcctgaact acgacaactt catgcaagcc
      361 gtgcgcttgg tggtggaagc ggttggcatt cccgagtcgc gcatgacggt ttcgacttcg
      421 ggcatcgtgc cgcgcatcgc cgatttcggg gccgagcctg tccgtcccaa gctcgcggtg
      481 tcgctgaacg ctcccaacga ggcgctgcgc acgcaagtca tgccgatcac gaagaagtgg
      541 accatcgaga agctgatggc agcgctgcgc gagttccctc tgcgcaatcg cgagcggctg
      601 accttcgagt acgtcctgct gggcggggtc aacgatggcg ttcagcacgc ccgcgagatg
      661 gtcgacctca ttcgtggtct gcgggccaag gtaaacctga ttgcgctgaa ttccggcccg
      721 gaaattccct tctcgacgcc gtcggaggag cgcgtgctgg cgtttcaaag cgtgctcacg
      781 cgagccggaa ttccggcgtt cgttcgccgt ccccgtggcc gcgacatcta tgccgcctgc
      841 gggcagctca aacacacaac ggaactggtc caggtgacgg ggtagatccg agacgaggga
      901 caccagcgga cgtgactgcc ccgccctcgc caaaatcagg ctagaacgtg cccccagatt
      961 tgactttcgt ttggacactt ttacctttct ggtccttccc aatttcgtgc aacgtcggcc
     1021 cctcccagca catctaaacc ttgacaacga ttcactcaag ttctatagtc ggtttgttat
     1081 aaaggcttga gatgcaatca agtgcattta ttatcagtaa cttacaagct aattaagcca
     1141 ggagggtggc tgcaatggga aagattatcg gaattgactt gggaaccacc aactcggtgg
     1201 ttgccgtcat ggaaggcggc gagcccaaag tcatccctaa cgaagagggc gggcgaacca
     1261 cgccttcggt cgtgggattc accaagaatg gggaacggct ggtcggccag gtcgccaagc
     1321 gccaggccat taccaatccg gagaacaccg tctactccat caagcgcttc atgggacgcc
     1381 gctacgacga agtcaatgaa gagatgaaga tggtgcctta caaggtggtg cagtcgggcg
     1441 atcacgtggc catcgtggcg caggggaagg aatatacccc gccgcaggtc tcggcaatga
     1501 ttctgcagaa gctgaagaaa tcggcggaag actatctcgg ccagacggtg acggatgccg
     1561 tgatcacggt cccggcctac ttcaacgacg cgcagcgcca ggcgaccaaa gacgcgggca
     1621 agattgccgg tcttgacgtc aagcgcatca tcaacgagcc gacggcggcg gcgctggcct
     1681 acgggctcga caagaagaag gacgaaacca tcgccgtgta tgacttcggc ggcggcacct
     1741 tcgacatttc gattctcgaa gtcggcgaag gcgtcattga ggtgaagtcc accaacggcg
     1801 acacgcacct gggcggcgac aacatcgacc agcgcattgt ggactggttg atcgacgagt
     1861 tcaagaagga cgaaggcctg agcctgcgcg acaagggcaa cgagatggcg ctgcaacgtc
     1921 tgcgcgacgg ggccgaacgc gccaagatcg agctttccac caccatggag accgaaatca
     1981 acctgccctt tatcacggcg gatgcgagcg gtcccaagca cctggtcaag aagctgactc
     2041 gctccaagct ggaagagatg gtgaaggaca tcatcgagcg gtcgatgggg ccttgcaagg
     2101 cggccatcaa ggacgccggc atctctcccg acaaaatcga cgaagtagtc ctggtaggcg
     2161 gacagacctg catgccgcgc atccagcagt tggtgaagga gctgtttggc aaagagccgc
     2221 acaagggcgt gaaccccgac gaagtggtcg cgattggcgc agccatccag ggcggagtgc
     2281 tcggcggcga ggtgaaagac ctgctgttgc tcgacgtgac cccgctgacg ctgtcgattg
     2341 agaccatggg cggcgtggcc acgaacatga tcccgcgcaa cacgaccatt ccgacgaaga
     2401 agacggaaac cttctcgacg gcggccgaca accagacgtc ggtcgaggta catgtgctgc
     2461 agggcgagcg tccgatggcg cgcgacaacc gcactctcgg caagttccac ctgaccggac
     2521 tgcctccggc tccgcgtggc ctgcctcaga tcgaggtgac gttcgacatc gatgccaacg
     2581 gtatcctgaa cgtcaatgcc aaggacatgg ccacgggcaa ggaccagaag attacgatca
     2641 cgtcgtcttc cggcctgagc aaggacgagg tggagcgcat ggccaaggac gccgatgcgc
     2701 acgcggccga ggacaagg
//
LOCUS       DATMGX010000745         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_292741, whole genome shotgun sequence.
ACCESSION   DATMGX010000745 DATMGX010000000
VERSION     DATMGX010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292741"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VNR65_12270"
     CDS             complement(<1..379)
                     /locus_tag="VNR65_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWJ19490.1"
                     /translation="MMKRVNQVGLWFVTALFLTSGVSHFLLTRFFVAIMPPYLPLPYA
                     AVYVSGAFEIIGAIGLLLPAYRRAAGIGLFVMTILVTPANIHMALNPELFPAFSKSLL
                     YWRLVAQVGLLALIYAVAIRRPHP"
     gene            1172..1546
                     /locus_tag="VNR65_12275"
     CDS             1172..1546
                     /locus_tag="VNR65_12275"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWJ19491.1"
                     /translation="MIRTLTFAAALAAAIALPAVAEDVKSPSNAGTVLTAQTAITEQE
                     AKAWVGRPVYSSDGKKLGEVAAILRTPDNKITELRADMGGFLGIGEHQIALPAALFAL
                     HNDRVVLNLTAAAAKGLPKVAK"
     gene            1856..>2332
                     /locus_tag="VNR65_12280"
     CDS             1856..>2332
                     /locus_tag="VNR65_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19492.1"
                     /translation="MIGFVFAVLNAGQEKFLATAPGTQPSSLLGLSYLAVILLLIVFT
                     NVRLRGINSVVALLAVAFIAVVLAWFGWWDDIAKLIPYLSVHMNTGFYVVFSTALFII
                     WLLMFFVFDRLTYWRIRPAQMTEEHLIGGGAESFDTNGLRFQKLAPICSGRRLVSAP"
BASE COUNT          518 a          639 c          671 g          504 t
ORIGIN      
        1 gtgggtgcgg ccgtcggatt gcgacggcgt agatcaaggc cagcaggccc acctgtgcca
       61 caaggcgcca gtagagcagg ctcttggaaa aagcagggaa tagctctgga ttgagcgcca
      121 tatgaatatt ggccggcgtc acgagaatag tcatgacaaa gagcccgatg cctgctgcgc
      181 ggcggtaggc tgggagaagc aatccaatcg cacctatgat ttcgaaggcc ccgctgacgt
      241 aaaccgcagc ataaggcaat ggcagataag gcggcatgat cgcgacaaaa aagcgggtca
      301 gcaggaaatg gctcaccccg cttgtcagaa acagcgctgt gacaaaccat aggccgactt
      361 ggttgacacg tttcatcatt tgcctcccgc gtcgtcgacg gccgcgcgcc agcgcaaggg
      421 acgtccaagc gctgtgatga gcccgagatg ttgattggct cgcggccagg ggtcgatcat
      481 catgctggaa ccatttgaaa atgtacccgc agtagatagt cgcgtagtgg gaatgcattc
      541 gggagtattg gcgtaggatt tcgggaagct tctggccccc ataagccgct acatcgcggt
      601 gtccacgatg cgatgcaatg gcctggcgaa taactggggc gcagaacctc gctttgagga
      661 agcccaggac tcaacttggc tttggttggc gcgccgaaca agaaagcggc cgcgagaatc
      721 cgcagcggct ttcccttccg cgtatccgca tctgcgcagc caaacaacgc atacttccca
      781 acaccggctt tgctgaagtt taaaaccagc gcccccggcc gtaataaccg ccgccaagca
      841 ggagtaccac aatgacgata agcaaaagtg tgtcgatgtt catagcgttt tccttgagtg
      901 tcgggtcaac tttgccacag cgcgacggtt taaaaatcgc gcgagcggtg ctcatcagga
      961 tgcggaggag atggccatta ggatgccgag taaaaagatt cggaatctac tcgcaattag
     1021 gagattgttt tcacacgaat tacgccatgt gttcagtaga gctctcggtt tctatcaata
     1081 catatccacg aaacctgagc actttccgag tctagacgct taagcgtttc tgtgaggaga
     1141 tgattttcat atcatattag gagttcacgt gatgatacgt acactgacat ttgcggccgc
     1201 gcttgccgcc gcgatcgcgc tcccggcagt tgctgaggac gtcaaatctc cctccaatgc
     1261 cggtacggtc ctcaccgctc aaacggcgat caccgagcaa gaggccaagg cctgggtcgg
     1321 tagacccgta tatagcagcg acggcaaaaa actcggtgag gtcgcagcaa tcctgcgaac
     1381 gccagacaac aagatcaccg aattgcgcgc cgatatgggc ggcttcctgg gcatcggcga
     1441 gcatcaaatc gccctcccgg cggccctttt tgccttacac aatgaccgcg ttgtgctcaa
     1501 cctgacggcg gcggcggcaa agggacttcc gaaggttgcg aagtaatcat ccaacgcaaa
     1561 ggggggtggg cgttcggcgt gctcccccaa tttccttttt gcggcggcgc aatctcagaa
     1621 atctgcgacc gagctcaagc gccccgggcc ctattgaggg ctgcgcggca ggcctcgatg
     1681 ccggaaggcg gggccgagtt aagctcgatc cggacttaac ctcaggggat atgaagccat
     1741 gacacaccag ctcacgagcg atcaaccgaa taccgaggcg ttgcctcatc aggcggctcc
     1801 gcgtgagatt gtcatttatg gacactccac gcttttcttc tggtggccgg cttgggtgat
     1861 aggcttcgtg tttgcagtct taaacgccgg gcaggaaaag tttctagcga ccgctccggg
     1921 gacccagcca agttcgttgc ttggtcttag ttacctggcg gtgattttat tactgattgt
     1981 tttcacaaat gtccggctgc gtggcatcaa ttcggtcgtg gcgttgctcg ctgtcgcatt
     2041 tatcgcggtc gtgctcgcct ggttcggctg gtgggacgac atcgccaaac tgatcccgta
     2101 tctatcggtg catatgaaca ccggctttta tgtcgtgttc tcgacagccc tattcatcat
     2161 ctggctgcta atgttctttg tgttcgaccg tctaacctac tggcgcatac ggccagcgca
     2221 gatgaccgaa gagcacctga tcggcggcgg cgcggagagc tttgacacca acggattgcg
     2281 ctttcaaaag ctagcgccga tttgttccgg tcgacgcttg gtttcggcgc ca
//
LOCUS       DATMGX010000746         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_473504, whole genome shotgun sequence.
ACCESSION   DATMGX010000746 DATMGX010000000
VERSION     DATMGX010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473504"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..560
                     /locus_tag="VNR65_12285"
     CDS             99..560
                     /locus_tag="VNR65_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWJ19493.1"
                     /translation="MAKPLHPHSPSFDAISELILEVFRLNGRLLEAGDALVADIALTS
                     ARWQVMGAIALSPLSLPVAHLARNMGLSRQSVQRLANEMEKDGLLMFAPNPHHERAKL
                     VLLTGRGTEVYREAMARQSPWAAKLASGIEAKDLEATRSLLHELRMRLSHE"
     gene            611..1081
                     /locus_tag="VNR65_12290"
     CDS             611..1081
                     /locus_tag="VNR65_12290"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HWJ19494.1"
                     /translation="MEYSILRFLHVLGAVLIGGGLIGVWMADLRSRQLRELHGFAEAV
                     RNIAVFYDGIVVPGALLLMASGTWLIIQYHGGWAFLDVPWLAGMVALFAFEFIEGNTI
                     TRLYFMRLRRLTKTALAKGSFTPELEAARAEGLPTFTHFLDLPLVFLIIALGVM"
     gene            complement(1310..>2240)
                     /gene="fabF"
                     /locus_tag="VNR65_12295"
     CDS             complement(1310..>2240)
                     /gene="fabF"
                     /locus_tag="VNR65_12295"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWJ19495.1"
                     /translation="GSGIGGLQGIERATMTVHEKGPRRLSPFFIPGRLINLASGYVSI
                     KHGLKGPNHAVVTACSTGAHAIGDAARLIALDDADVMVAGGTESPLCRLALAGFAACK
                     ALSTHFNDDPLRASRPYDRDRDGFVMGEGAGVVVLESYEHAKARGAKMYAEVIGYGMS
                     GDAYHITAPAENGDGAFRCMKAAMKRANVGPADIDYVNAHGTSTPLGDEIELGAVERL
                     FGDAAGTLAMSSTKSATGHLLGAAGAVEAIFSVLSIKNNIAPPTINLDNPSVNTSIDL
                     VPHTPKQKQIDTVLSNSFGFGGTNASLILRRVD"
BASE COUNT          430 a          672 c          660 g          478 t
ORIGIN      
        1 tgtacaccgg ccgtacgggt ctccggcgac ggagggagaa aggtcagctt tttgctacta
       61 tttgacaaca tgctgtctaa ctgacatgat tgtgtcaaat ggctaagccc cttcatcccc
      121 actccccgtc tttcgacgcg ataagcgaac tcattctgga ggtttttcgc ctgaatggtc
      181 gattgctgga agccggcgac gcgcttgtgg ccgatatcgc gctgacgagc gcccgctggc
      241 aggtcatggg agcgatcgcg ctttcgccct tgtcgctgcc ggtcgcgcat cttgctcgca
      301 atatgggcct ttcgcgccaa tcggtacagc ggctggcgaa cgagatggag aaggacgggc
      361 tcttgatgtt cgctccgaac cctcaccatg aacgagccaa actcgtactt ctcacggggc
      421 gaggcactga ggtctatcgc gaggcgatgg ctcggcagtc gccatgggcc gccaaattgg
      481 cctccggcat cgaggcgaag gatcttgaag ccactcggag cctgcttcac gagcttcgaa
      541 tgaggctttc ccatgaatag aggacggata ggcagcgccg gcgagcgggc ttgtcggaca
      601 ggagagtgtt atggagtaca gtattctcag atttctccac gtgctcggcg ccgtgctgat
      661 tggcgggggc ctcattggcg tgtggatggc tgaccttcgc tctcgccagc tgagggagct
      721 tcacggcttt gccgaagcgg tccgcaacat tgcagttttc tacgacggaa tcgtggtgcc
      781 gggcgctctc ctgctcatgg cctcgggcac atggctcatc atacaatatc acggaggctg
      841 ggcctttctg gacgtgccgt ggctcgcggg aatggttgcg cttttcgcct tcgagttcat
      901 cgaaggaaac acgataaccc ggctctactt catgcggctt cggaggttga caaagaccgc
      961 gctggcaaaa ggctcgttca cgccggaact tgaggctgcc cgcgccgagg gccttcccac
     1021 ctttacgcat ttcctcgatt tgccgctggt gttcctgatt atcgccttag gggttatgta
     1081 gcccgacagt tggtcggtgt tcctcttcgg ctgcgcgaca gccatactcg ttgcgaccgt
     1141 tttgaccgtg tcaattccca ggctctatat ttggcaggaa aaggcggggt agccggcaac
     1201 ccaggctgag catggggcgc caaacgaagc accattcgtc gctctaacac ttcagaatcg
     1261 agcaactttt ccgcccttta atcgatttcc gatttaaggg cgggtcgatc taatcaaccc
     1321 gccgcagaat caagcttgca ttcgtcccgc cgaagccgaa ggaattgctg agcacggtgt
     1381 cgatctgctt ctgtttcgga gtgtgaggca caagatcaat cgaggtattg acggaagggt
     1441 tatcgagatt gatcgtgggg ggcgcgatgt tgttcttgat agagagcact gaaaagatcg
     1501 cctcaacagc tccggccgcg ccaagcagat gcccggtcgc cgattttgtc gatgacatcg
     1561 ccagcgtgcc cgccgcatcc ccgaacaacc gctcgaccgc cccaagctcg atctcgtcgc
     1621 ccaagggtgt cgaggttccg tgcgcgttca catagtcgat gtcagccggc ccgacattcg
     1681 cccgcttcat cgccgctttc atgcagcgga acgcgccatc gccattctcg gctggcgcag
     1741 tgatatgata cgcatccccc gacatgccgt agccgatgac ttcggcgtac atcttcgccc
     1801 cgcgcgcctt tgcatgctca taactttcga gcacaacgac gccggcgccc tcccccatca
     1861 cgaagccgtc gcgatcacgg tcataaggcc gggaggctcg cagcggatcg tcgttgaaat
     1921 gggtcgacaa cgctttgcag gcggcaaacc ccgccaaagc aaggcggcaa agcgggctct
     1981 cggtcccacc cgcaaccatc acgtcggcat cgtcgagggc aatgaggcgt gcagcgtccc
     2041 cgatggcatg tgcgcccgtc gagcaggccg tgaccacagc atgattcggc cctttcaagc
     2101 cgtgctttat ggacacgtaa ccggacgcga gattaataag ccgaccgggg atgaagaacg
     2161 ggctgagacg gcgcggcccc ttctcgtgaa ccgtcatcgt ggcgcgttca attccctgaa
     2221 ggcctccgat gccggacccg
//
LOCUS       DATMGX010000747         2462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_755,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000747 DATMGX010000000
VERSION     DATMGX010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VNR65_12300"
     CDS             <1..1160
                     /locus_tag="VNR65_12300"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWJ19496.1"
                     /translation="RFKVVDTGTALEAAKAFAEGQTDLAVVRGDVGDLSKGGSVVVVA
                     HVVALVIAPPGSSISSMDALKGHSVGVVGGEANSKIVDVLAKEYGLVRTKVFKDIALA
                     DARRALQSKEVSAILVVIPLSEKYLTLVRGLFQQNAKTSPALIPIESAGAIAEADRAY
                     ESFDVPKGTLRGSPPVPEDDLTTLRTSLYLIADKKLSTDLVGSLTQTIMKVRRDLLIE
                     DPIFAQITAPSTDADANVPLHPGAAAFYNGTQQSFMDEYGNWIYLAPMILGGVATVLA
                     AAWKFLGIGQPASDGPLDTLYALARRIRKADTEAELSDTEEEIDGILNAERARAASGE
                     EGAVDATTLNVAAHRLEDLTHDRRSIITGKPPIAIIPGGGSARQVDAIRAK"
     gene            2150..>2462
                     /locus_tag="VNR65_12305"
     CDS             2150..>2462
                     /locus_tag="VNR65_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19497.1"
                     /translation="MSKLLSLSIILASALSSGATAQQINGTPGSPSATITIDGKQLPP
                     PPQKFGGVIKETAKESQPWWPPRVVPPKGAPNVLLIMTDDQGYGVSGTFGGVIPTPAL
                     DR"
BASE COUNT          563 a          672 c          745 g          482 t
ORIGIN      
        1 tgagattcaa ggtcgttgat actggtaccg ccctggaggc tgcaaaagcg tttgcggaag
       61 ggcagaccga cctggctgtc gtccgtggcg acgtcggtga cttgtcgaag ggcggctcgg
      121 ttgtggtcgt ggctcacgta gtcgcattgg tcatcgcgcc cccgggatcg tccatttcta
      181 gcatggacgc actaaagggc catagcgttg gcgtggttgg aggggaagcg aattccaaaa
      241 tcgtcgacgt cctggcgaag gaatatggcc tggtccgtac gaaggtcttc aaggatatcg
      301 ctctggcgga cgctcggcgg gccctccaat cgaaggaagt aagcgccatt cttgtcgtaa
      361 tacccctgag tgaaaaatat ttgacactcg tgcgaggact ctttcagcag aatgcaaaga
      421 cctccccggc actaattccc atcgaatccg ccggcgcaat agcagaagcg gaccgcgcct
      481 atgaaagctt cgacgtgccg aaaggtacgt tgcgcggatc tccacctgta ccggaggacg
      541 atcttacgac gctgaggacc tcgttgtacc tgatagcaga caagaagctt agtactgact
      601 tggttggcag cctgacgcag accatcatga aggtgcgaag ggaccttttg atagaggatc
      661 cgatcttcgc gcaaatcacg gcgcccagca ccgacgccga cgccaacgtg cccttgcatc
      721 cgggagcggc tgctttctac aacggaacgc aacaaagctt catggatgaa tacggcaact
      781 ggatctatct ggcgccaatg attttgggcg gcgtcgcaac ggtcctcgca gcagcctgga
      841 aatttctggg aattggccaa cctgcaagtg atggtccgct tgacaccctc tacgccctgg
      901 cccgccgaat acggaaagcc gatacggagg ccgaactgtc agacaccgaa gaggaaatcg
      961 acggcatact caacgccgaa cgggccaggg ctgcaagcgg tgaagagggg gcggtcgacg
     1021 ccaccacatt gaacgtcgcg gcccaccgat tggaagacct gactcacgat cgacggtcga
     1081 tcattactgg aaagcccccg atcgcgatca tcccaggagg ggggagcgcg aggcaagtgg
     1141 acgcaattcg cgcgaaataa gcagttggat cgaataaatg gctggcaacg tggttccctt
     1201 gagacgcagc agccagattg agctggcgaa actctatttg catttgctga atgctgtact
     1261 tgacgaacgg gcgtcaaccg cttccgatgc gtcggcaagt cagccatcgt gttccgatga
     1321 tgacacgtcg tcttcgttcc gctgtccacg gcaaccggaa tgcggcgctt gagccgtgat
     1381 gggctaacct tatccgagat ctggggaagc aaccagagac catccacagg aggcctctgg
     1441 ccgcaagcgg ttcgttggat gcggtgtggt ccgcatcgcg cttatacgca tcggcgaacg
     1501 cgaacgccgt tcacaacgac atagtgacag gcggcgaccg gcggcggcct gacaacgacg
     1561 gctgccccgc gggccagcgc aacccgcacg atagacgccg gcggcacaga cgacggcatc
     1621 ggcgttagtg gttgcgattg tcattgttga ggtaaacgca gcgacggcga gtatggcgat
     1681 cgaaagcggg cgcatattga acctccttgc agtatgacct gccaccagga attcttgcct
     1741 cgcaagaagc aggcaaaagc tggtccaaga aaattacggc tcaatcgccc gtcaaatcaa
     1801 agaacaggcg aatatccggc gcaatcacgg cgctctccgg tcaatcacaa cccacgaaat
     1861 gtcggagtga ctccgaaaat ttggagggag ttgaactcgg gagcgtgtgg gatccgcgcg
     1921 gagatctcat tgccaaactg aatttgcgga cgttttctac aaaggtccgt cccatcgcgt
     1981 gctggcccgc ttcttgcgaa ggggtgcgtg agattgtgat ctacggactg cgtgtcagag
     2041 aaatggactt ggttgagtcc cttacgttga tgtgatcgaa cgggcggtct tgtgatcgtg
     2101 agtgtgaccg gatgttttag gtgccaaccg ggagaataag atgaccgaaa tgtcaaagct
     2161 gctgtcgttg agcattattc ttgcttcagc cctttcgtcc ggagcaacgg cgcagcaaat
     2221 caatggcact ccgggttcgc ccagcgcgac aatcaccatc gatggtaaac agctcccgcc
     2281 gccgcctcag aaatttggag gagtcatcaa ggaaaccgcc aaggagtcgc agccgtggtg
     2341 gccgccgcgc gtggtgccgc cgaaaggcgc gcccaacgtg ctgcttatca tgaccgacga
     2401 tcaggggtat ggcgtaagcg gtacctttgg tggtgttatt ccgacgcctg cgctcgatcg
     2461 aa
//
LOCUS       DATMGX010000748         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_256256, whole genome shotgun sequence.
ACCESSION   DATMGX010000748 DATMGX010000000
VERSION     DATMGX010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256256"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..515)
                     /locus_tag="VNR65_12310"
     CDS             complement(42..515)
                     /locus_tag="VNR65_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWJ19498.1"
                     /translation="MSKDSLYLDDLSPGMRFTAGPITLTEDAIIAFAREFDPQSFHTD
                     PETAATSFFRGHVASGWQTAAVTMRMLVDGAMPFAGGAIGLGVEISWPRAVRPGDALS
                     VVLEIMDITPSSSKPGRAVATLKTTTTNQNGEIVQVMTSKNIVFKRGYLPGERKG"
     gene            complement(640..1590)
                     /locus_tag="VNR65_12315"
     CDS             complement(640..1590)
                     /locus_tag="VNR65_12315"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWJ19499.1"
                     /translation="MIVKADKAAITEAARIILAGGLVAFPTETVYGLGADATQGQAVA
                     RIFEAKGRPRFNPLIAHVLDLQAALGLAEFDEKALALARDFWPGPLTLVLPLSQHSAH
                     RISELVTAGLDTIAIRIPAHPVARELLAAVAVPIAAPSANKSGRVSPTQAEHVETDFG
                     GEVDMILDGGSSNAGLESTIVTLAPQPTLLRPGAIAREDIEAVLGCRLADFRQGKVQS
                     PGQLLSHYAPRAALRLNAVAALPGEALLGFGPLAPKTALNLSPTGDLREAAANLFASL
                     RTLDAQKPSGIAVMPIPEAGLGEAINDRLRRASVPKQSKF"
     gene            1829..>2008
                     /locus_tag="VNR65_12320"
     CDS             1829..>2008
                     /locus_tag="VNR65_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HWJ19500.1"
                     /translation="MSGIRLLSVALALMAAVAFQVSQADARIGGGSSFGSRGSRTYTA
                     PPSTSTAPGQAAPMNR"
BASE COUNT          411 a          594 c          627 g          376 t
ORIGIN      
        1 cttcgcgctc tcatcgacat tccgtcccac tccgagcggg actagccttt tctttcgccc
       61 ggcaagtaac cgcgcttgaa gacaatgttc ttcgaggtca tcacctgcac gatttccccg
      121 ttctggttcg tcgttgtcgt cttgagagta gcaacggccc ggccgggttt cgatgatgac
      181 ggcgtgatgt ccatgatttc cagcacgacg gaaagggcat cgccgggccg gaccgccctt
      241 ggccaggaaa tttcgacgcc caacccgatc gcgccgcccg cgaagggcat cgcgccatcg
      301 accagcatcc gcatggtcac tgccgctgtc tgccagccac tcgcgacatg ccctctaaag
      361 aaagacgtcg ccgcggtctc agggtccgta tgaaaggatt gcggatcgaa ctctcttgca
      421 aaagcgatga tcgcatcctc cgtgagggtg atcgggccgg ctgtaaagcg catgccgggc
      481 gagagatcgt cgaggtagag cgaatccttg gacattgggg agcaccttgc ggaaaagctt
      541 gcggcgtcga actgttcgaa aatagtgaac gacgatctca cggcaatagg ggatgcagac
      601 cacacccgga atgcaaaacg gcgtttcgac gcagattgat cagaacttgg actgttttgg
      661 tacacttgcc cggcggagac ggtcgttaat ggcttcgccg agaccggctt ctggaatggg
      721 catgaccgct atcccggagg gcttctgcgc gtcgagcgtt cgcagagacg caaaaagatt
      781 ggctgcggct tcgcgcaaat ctccggttgg actgaggttg agggccgtct tgggcgcgag
      841 cggtccaaac cccaaaagcg cctcgcccgg caaagcggcc acagcgttga gccgcagcgc
      901 cgcccgaggc gcataatggc tgagaagctg gcccggcgat tgcactttac cctggcggaa
      961 gtccgcgaga cggcagccaa gaaccgcttc gatatcctcg cgggcaattg cccccgggcg
     1021 caagagggta ggctgtggcg cgagcgtcac aatcgtggat tcaaggcctg cgttcgacga
     1081 gccgccatcg aggatcatgt ccacttcgcc gccaaaatcg gtttcgacat gttccgcttg
     1141 cgttgggctc acgcgcccgg atttgttggc gctcggagca gcaatcggga cggcaacggc
     1201 tgcaagaagc tcgcgggcga cagggtgggc tggaatgcgg atggcgatcg tgtcgagccc
     1261 ggcggtgacg agttcgctga tgcgatgggc ggaatgctgc gacagcggca gaacgagggt
     1321 gagcggcccg ggccagaaat cgcgggccaa cgccagcgct ttttcgtcaa actcggcaag
     1381 gccgagggcc gcttggagat ccaagacatg cgcgatcagg ggattgaaac gggggcggcc
     1441 cttggcttca aaaatgcgcg cgacggcttg gccctgtgtg gcgtccgcgc cgagcccata
     1501 aaccgtctcg gtcgggaacg cgacaaggcc gcccgcgagg atgatccgtg cggcctctgt
     1561 aatcgccgcc ttgtccgcct taacgatcat gtcagctcaa tgtgccgctg caaaggatag
     1621 gtctctacta caaatccgta gaggaccgaa atgtgtcctc ttcgacgccg atgggctaac
     1681 gatccgctgc aagcgcaaat tgccgtcgtg agctttcgcg ctcaattttg agtggccaca
     1741 aaaatgccat cgtgcaacct cgatctggtt aaaattgcgg catgacactc cacatagcct
     1801 ctaagaagtg agaacggaga ttccccacat gtcaggcatt cggttattga gcgtggcgtt
     1861 ggctttgatg gccgcggttg cgtttcaagt cagccaagcg gatgcgcgca tcggcggcgg
     1921 cagcagcttt ggcagccgcg gaagccgcac ctacaccgcg ccccccagca catccacagc
     1981 tcccggacaa gcggctccaa tgaaccgg
//
LOCUS       DATMGX010000749         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_305033, whole genome shotgun sequence.
ACCESSION   DATMGX010000749 DATMGX010000000
VERSION     DATMGX010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305033"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VNR65_12325"
     CDS             <1..976
                     /locus_tag="VNR65_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWJ19501.1"
                     /translation="NTITYAHAAEMPFAFAWVSDTHLYPKSLNSRFVDKAARAAKEVQ
                     AMNPPADFLIFGGDIAQLGRADELELGNQILQEVKIKKHFIPGEHDWYFDMGKKWGQL
                     YGQPNWTFDHKGVRFIGLDTVTRGPDYWSATKMTPEMRMDHMATLDGTVAGAWAGVGD
                     DQIGWMQSVLSDWNRDAPVIIFSHNPLYECYPPWNFWVRDWRQVHEVLKPYTKVTNIH
                     GHVHQVLYNEIGTTRSIGMLATAWNWPYPPEGVPKATRMMMRVDPGDIYDGVGWGIIS
                     QSENMRFEPQYVMWGRKEVFSSNPADVSSAHPEILRPRIADNGWFWKD"
     gene            1047..1502
                     /locus_tag="VNR65_12330"
     CDS             1047..1502
                     /locus_tag="VNR65_12330"
                     /inference="COORDINATES: protein motif:HMM:NF044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19502.1"
                     /translation="MNALYQTRCLLAGAGLTVIAFSICGPVLAERKPTTPQQLKQYED
                     AFMEEVKKGDLLFHGNAELAKKMGVTLSTTGMACAMCHPMASDTHPGSFPKFQPTMGK
                     FAALRDMVNWCIEKPMQGEQIETDSAAMKAIEAYIYWSNSGSVLDTGKY"
     gene            1548..>2370
                     /locus_tag="VNR65_12335"
     CDS             1548..>2370
                     /locus_tag="VNR65_12335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HWJ19503.1"
                     /translation="MLSRRHFVAGSAALGVGAAIGRPALAGTKPKVVIVGGGPGGGSV
                     MRALAAAAPGKIDITLIEAQQAYTACFQSNLYVGGFRPLDAFTFSFEALRRLEGVTVI
                     NDTAARISPETRTVQLLSGSEVPYDLLVTAPGIDLDYTSVPGWSKDAEERMPHAYKGG
                     AQLRLLKEQLNVVSDRGLIVIIAPPNPYRCPPGPYERASMMARALKAAGKTARIVILD
                     AKEAFSKQPLFEAGWQRHYPGMIEWLPPSIHSGIKSVNPATMTVETGFEVYANAAL"
BASE COUNT          502 a          689 c          708 g          471 t
ORIGIN      
        1 caacaccata acttatgcgc acgccgccga gatgcccttc gccttcgctt gggtctcgga
       61 cacgcatctc tacccgaaaa gcctgaacag ccgtttcgtc gataaggcgg cgcgtgcggc
      121 gaaggaagtt caggccatga atcctccggc cgactttctg attttcggcg gcgacatcgc
      181 ccagctcgga cgcgcagacg aactcgaact cgggaaccag atcctgcagg aagtgaagat
      241 caagaaacac ttcattcccg gcgagcatga ctggtacttc gacatgggca agaagtgggg
      301 ccagctttac gggcagccca actggacttt cgaccataaa ggcgtccgct tcatcggcct
      361 cgatacggtg acgcgcgggc cggattactg gtccgctaca aagatgacgc cggaaatgcg
      421 aatggaccat atggccacgc tggacggaac ggtggcaggc gcctgggcag gcgtgggcga
      481 cgatcagatc ggctggatgc aaagcgtcct gtcggattgg aatagggatg cgcccgttat
      541 catcttcagc cataacccgc tctatgaatg ttatccacct tggaatttct gggtccgcga
      601 ctggcggcaa gtccatgaag tgctaaagcc ctacaccaag gtgacgaaca tccatggtca
      661 cgtgcaccag gttctctata acgagatagg cacgacacgc tcgatcggca tgctcgccac
      721 ggcttggaac tggccctatc ctcctgaagg cgtgccaaag gctacgcgga tgatgatgcg
      781 cgttgatccg ggcgacatct atgatggcgt cggctggggt atcatcagtc agtcagaaaa
      841 catgcgcttc gaaccgcaat atgtgatgtg gggccgcaag gaggtgtttt cctccaatcc
      901 ggcggacgtc tcgtccgccc accctgaaat tttacggccg cgcatcgcag acaacggctg
      961 gttctggaaa gattagaaat ggtggcacgt ggcagcttgg cctcgggccg cgccgcgctg
     1021 catcggatcc tcattgggag agtcttatga acgccctcta ccaaacccgc tgccttctcg
     1081 ccggtgctgg gctgacagta atcgcattca gcatctgcgg cccggtcctt gcagagcgca
     1141 aaccgacgac gcctcagcag ttgaaacagt acgaagacgc cttcatggag gaggtgaaaa
     1201 aaggagacct tctcttccac ggcaatgctg agttagcgaa gaagatgggc gtcacgctct
     1261 ccacgacggg gatggcctgc gccatgtgcc accccatggc gtccgatacg catccaggct
     1321 ccttccccaa atttcagccg accatgggca aatttgcggc tctgcgcgat atggtgaact
     1381 ggtgtatcga aaagcccatg cagggcgagc aaatagaaac cgattcggca gcgatgaagg
     1441 cgatagaagc ctatatctac tggtccaact cagggtcggt gcttgatact ggcaaatatt
     1501 agcggcgttg gaatggttct gaccactatg tccttttcct gcagacgatg ctgagccgga
     1561 ggcattttgt agccgggagc gcggcgcttg gcgtcggcgc tgccatcggc aggcctgccc
     1621 ttgcggggac aaagccgaaa gttgttatag ttggcggggg ccccggcggc ggctcggtga
     1681 tgcgtgcgct cgccgctgcc gcgcctggga aaatagacat tacgctgatc gaggcccagc
     1741 aggcttatac ggcttgcttc cagtccaacc tctatgttgg cgggttccgg ccgctggatg
     1801 cattcacgtt ctccttcgag gctctaagaa ggctcgaggg cgtgaccgtc atcaatgata
     1861 ccgctgcgcg catcagcccg gaaacccgca ccgttcagct gctcagtgga tccgaggttc
     1921 cgtacgatct cctcgtgacg gcgccgggca tcgatcttga ttacacatct gttccgggct
     1981 ggtctaagga cgcggaagag cgcatgccgc atgcttacaa gggcggcgcg cagttgaggc
     2041 tgctgaagga acagctcaac gtcgtttccg accgcggatt aatcgtcatc atcgcgcccc
     2101 caaatccata tcgctgcccg cccggaccct atgagcgcgc atccatgatg gcgcgtgccc
     2161 ttaaagcggc cgggaaaacg gcgcggatcg tcatcctcga cgccaaggag gcgttttcaa
     2221 agcagccctt gtttgaggcg ggctggcagc ggcattaccc ggggatgatc gagtggctcc
     2281 ccccatccat ccacagcggg atcaagtcgg ttaatcccgc aacgatgacg gtcgaaaccg
     2341 ggttcgaggt ctacgcgaat gcggcgctgg
//
LOCUS       DATMGX010000750         2815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_223430, whole genome shotgun sequence.
ACCESSION   DATMGX010000750 DATMGX010000000
VERSION     DATMGX010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223430"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..781
                     /locus_tag="VNR65_12340"
     CDS             320..781
                     /locus_tag="VNR65_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ19504.1"
                     /translation="MPQLQLPMFAAGLTPITEDLAFQQEDGKVVYFHGLMPVFQHDEK
                     DLKSFRMFTSQLIANGTARQRDIARAFGVPLATVKRYMKVHREQGAAGFFRPRRRRSA
                     SVLTPEVKQRAQALLDEGKSVPEVSLAVGVAGDTLHKAIDAGRLHQSKKKN"
     gene            844..2559
                     /locus_tag="VNR65_12345"
     CDS             844..2559
                     /locus_tag="VNR65_12345"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19505.1"
                     /translation="MGYGATRSLERVAAAMGALEAASVIFEAARDIPQGGVLLALPAL
                     LAVGLLRHSEELCALPPGFYGQSSVMVLLALMALARIGSVEQLRYAAAGEWGNLLGLD
                     RIPEVRTLREKLKLLCTEPGRAARWNAVLAQEWIGQHETQQSSGLAFYIDGHVRVYHG
                     DLSKLPRHYVPRERFYLRATVDYWVNALDGQPFCYFNQAVDHGLVQALRGDVLPWLTA
                     NVPVSAEHEQRMAADPRVPRFTVVVDREGYSPDLFLELRPQRIAVLTYHRYPEGEDWR
                     AEEFQEQTVKLANGESVRMQLAERGTRLGRRPGVWVREVRKLGTAGHQVSIVSTNFSG
                     EAVSQAVALMARWSQENFFKYMREHFGLDALVQYGTEQIPATVTVLNPAWQGLNREVR
                     KKHRELRSLQKRQQNVGIQQPLSEAVVRTYEQHQGNLQESIEQLQPALDLLKQQRRQT
                     PRQIPVQDLPEPDRFTRLRTERKYFLDTIKMIAYRAETSMACTIREQLKRTDDARALL
                     RQIYDTEIDLMPDLEAKTLTVHLHHLTQAAHDIAVRHLCNELTATETIFPGTDLRLVY
                     KLGSP"
BASE COUNT          567 a          802 c          915 g          531 t
ORIGIN      
        1 tggctacgat cggtgtctag atcggctcga atcgtggcaa atgtcggcat cttgcacttc
       61 tagattctac agatctgccg ccgagttcat ttttactgaa ttattgcgtc cgagggccgg
      121 ctcgccgccg tgctccaact ctcgcgaagc ctaccgagcc gcgactggcc cacgtggcgc
      181 gggctttagc ctgctgagcc gagattccga actcctgaag tcatcccggc attttgggca
      241 gtccaggaaa atgacctgac ccaaattggg gtcatcctgg gtggttgcgc ggcacggctc
      301 gcaaaggaga aactgagcca tgccgcagct tcagttgccg atgtttgccg ctggtttgac
      361 ccccatcacc gaggacctgg cgtttcagca agaggatggg aaggtggtgt acttccacgg
      421 gctgatgccg gtgtttcagc acgacgaaaa agacctgaag agcttccgca tgtttaccag
      481 ccagttgatc gccaatggga cggcgaggca gcgggatatc gcgcgggcgt ttggggtacc
      541 gttggcaacg gtgaagcgct acatgaaggt gcatcgggaa caaggagccg ccggtttttt
      601 ccggccacgg cggcggcgtt cggcgagcgt gctcacgccg gaagtgaagc agcgcgcgca
      661 ggccttgttg gacgagggca aaagcgtgcc cgaggtgagt ctggcagtgg gcgtagcggg
      721 cgacacgctg cacaaagcga ttgacgccgg acggctgcac caatctaaaa aaaagaactg
      781 atacaggacc aagacggggc cagcaacaag agcgagcgta gcgaggtcga cagcgcggcg
      841 ccgatggggt acggcgccac ccggtcgctg gagcgggtgg cggcggcaat gggcgcgttg
      901 gaagcggcgt cggtgatctt cgaagcggcg cgggacatcc cgcaaggggg cgtgttgttg
      961 gcactgccgg cgctgttagc ggtgggcctg ctgcgccaca gcgaggagtt gtgcgccctg
     1021 cccccaggct tctacggcca gagcagcgtg atggtgctgc tggcgctgat ggcgctggcg
     1081 cgcatcggct ccgtcgagca gttgcgctat gcagcagcgg gagagtgggg caacctgctg
     1141 gggctggacc ggatcccgga agtgcgcacg ttgcgcgaga aactgaagct gctgtgcacc
     1201 gaaccgggcc gggcggcgcg ttggaacgcg gtcttggccc aagagtggat cggccagcac
     1261 gagacccaac aatcctccgg gctggcgttt tacatcgacg ggcacgtacg ggtctatcac
     1321 ggcgatttga gcaagctgcc acggcactat gtgccacgcg agcggttcta cctgcgcgcc
     1381 accgtggact actgggtgaa tgcgctggac ggtcaaccgt tctgttattt caaccaggcg
     1441 gtcgatcacg gtctggtgca ggccctgcgc ggagacgtgc tgccgtggct gacggccaac
     1501 gtaccggtgt cggccgaaca cgaacagcgc atggcggccg acccgcgggt gccgcgcttc
     1561 accgtggtcg tcgaccggga aggctacagc ccggacctat ttctggaatt acggccgcag
     1621 cggatcgcgg tgctgaccta ccatcgctac ccggaagggg aagactggag agcggaggag
     1681 ttccaggaac aaaccgtgaa actggcaaac ggcgagagcg tgcgtatgca gttggccgag
     1741 cgcggaacgc gtctgggccg gcgaccgggg gtgtgggtgc gggaagtgcg caagttgggc
     1801 accgcgggcc accaggtttc tatcgtgagc accaattttt cgggggaagc ggtttcgcag
     1861 gcggtggcct tgatggcgcg ctggtcgcag gaaaacttct tcaaatatat gcgggaacac
     1921 tttggtttag acgccctggt gcaatatggg accgaacaga ttcctgctac tgtgaccgtt
     1981 ctcaacccgg cgtggcaggg actgaatcgc gaagttcgca aaaagcacag ggagctgagg
     2041 agccttcaga aacggcaaca gaacgtgggc atccagcagc cgctatcaga agccgtggtt
     2101 cgtacttacg agcagcatca ggggaacctg caggaaagca tcgagcagct ccagccggca
     2161 ttggatctac tcaagcagca gcgccgacag actccccgcc agatcccggt tcaggacctg
     2221 ccggaaccgg accgcttcac gcgattgcgc acggagcgaa aatatttcct cgacaccatc
     2281 aaaatgatcg cgtatcgcgc cgaaaccagc atggcttgca ccatccgcga gcagctaaaa
     2341 cgcaccgacg acgcgcgtgc tttgctgcga caaatctacg acaccgagat cgatttgatg
     2401 cccgatctcg aggccaagac attgacggtc catctgcatc atctaacgca agccgctcat
     2461 gatattgcgg tccgccatct gtgcaacgaa ctcaccgcca cggagacgat ttttccgggc
     2521 accgatctcc ggctggtcta caaacttggc tcaccctaaa ttccacgaga tcaggagttc
     2581 ggagacttat ttcggctttt cttcccatcc cgatgcgaag cgactggtgg agacttgcaa
     2641 cttgcctcgc gggagttcat actccgacca tgaccctcaa gaatgcggcg ctactcgcgc
     2701 tggtcgggac tttgatcatt gcggcccttc gggtttggga tttcattttc accacccttg
     2761 gcttcgtgcg atgggcggtt tcggcggcgg aatttctttc gtcgttgatc tacgc
//
LOCUS       DATMGX010000751         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_70009, whole genome shotgun sequence.
ACCESSION   DATMGX010000751 DATMGX010000000
VERSION     DATMGX010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70009"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VNR65_12350"
     CDS             complement(<1..432)
                     /locus_tag="VNR65_12350"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWJ19506.1"
                     /translation="MSILVRIHWHLLSILFLGLLGTPVHAQQRIKLAVEPSQGVLAAG
                     QNSKAYLRVKLEGLPYEESASRPPVNVSLVIDRSGSMTGPKIEQAKEAAILALSRLSP
                     QDRVSVVAFDHRVEIPVPAGPFENFEEMKRRIEAIGPGGQTA"
     gene            complement(516..1070)
                     /locus_tag="VNR65_12355"
     CDS             complement(516..1070)
                     /locus_tag="VNR65_12355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ19507.1"
                     /translation="MADIVPLTETDAAERARLIHEATQIFFETANIKVFESVSAQDAF
                     YQRWFGHYLKTDPASFLLALDGNGAAIGYLAGCLDSFSEEARIIIDDISFYTPAFCSA
                     LRDYPSHFHINVKPGHQGQGVGRLLLTRFFQLCRERGSPGIHVVTGPKSRAVDFYRAC
                     GFVPLAAPEADSDLALLVYAIPAP"
     gene            complement(1109..1984)
                     /gene="fabI"
                     /locus_tag="VNR65_12360"
     CDS             complement(1109..1984)
                     /gene="fabI"
                     /locus_tag="VNR65_12360"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF005078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HWJ19508.1"
                     /translation="MTVVDIAKPSPLMAGKRGLIMGVANDRSIAYGIARVLAGHGAEL
                     AFTYQGDAFGRRAVPLAQGLGAKIILPCDVQDLNSVDAVFSELQSQWGSIDFLVHALA
                     FSDRNELKGRYADTTRENFVNTMVISCFSFTEIAKRAAALMKRGGSMITLTYGGSMRV
                     VPRYNVMGVAKAALEASVRYLAVDFGQQNIRVNGLSPGPMRTLAGSGVSDARVIFNYQ
                     KQHSPLGRTPALDEVGGAALYLLSDLSTAVTGEIHYVDAGYNIVAMPRIESLTESGEG
                     LADESANSHHKRAAE"
     gene            complement(2024..>2208)
                     /locus_tag="VNR65_12365"
     CDS             complement(2024..>2208)
                     /locus_tag="VNR65_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HWJ19509.1"
                     /translation="LMMNNGFVCESANIDELDPAFSDMPIVRERLDNVELNCVMSNSF
                     GFGGTNATLIFKRYDR"
BASE COUNT          486 a          641 c          655 g          426 t
ORIGIN      
        1 cgcagtctgg ccgcctggcc cgatagcctc gattcgtctt ttcatctcct cgaagttttc
       61 gaaggggccc gcgggaacgg ggatctccac gcggtgatcg aacgcgacga ccgacacgcg
      121 gtcctgcgga gacagtctcg aaagagcaag aatcgccgct tcttttgcct gctcgatttt
      181 agggcccgtc atagaaccgg accggtcgat cacaagcgag acgttgaccg gcgggcgcga
      241 cgccgattcc tcatagggaa ggccctcgag cttgaccctg agataggcct tactgttctg
      301 gccagcggcg agaacgccct ggcttggctc gaccgcgagc ttgatacgct gctgtgcatg
      361 aaccggagtg cccagcaaac caagaaaaag gatgcttaag agatgccaat gaatgcggac
      421 aagtatggac atggtgccat gacctttctt tgctccaagc caaagctatc gctattgttc
      481 ggcttgggcg gcaatcgggt cacgattggg ccaattcacg gcgcaggaat ggcgtaaacg
      541 agaagggcaa ggtcggagtc agcctcggga gccgcgagag gtacgaaccc gcatgcccgg
      601 tagaagtcga cggcgcgcga tttcgggccg gttacgacat gaatgccggg cgagccccgc
      661 tcgcggcaga gctgaaagaa gcgcgtaaga aggagccgtc cgacaccctg tccctgatgt
      721 ccaggcttca cattgatatg gaaatgagaa ggataatccc ggagcgcgga gcagaaagca
      781 ggcgtgtaga acgagatgtc atcgatgatg attcgcgctt cctccgagaa gctgtcgagg
      841 caacccgcca gatagccgat tgcggcaccg tttccgtcca gggccaaaag gaaagacgct
      901 ggatcggttt tgagatagtg gccgaaccag cgttgataga acgcatcttg cgccgacact
      961 gattcgaaga ccttgatgtt cgcggtctcg aagaagattt gcgtcgcctc gtggatcagc
     1021 cgcgcccgct ccgccgcatc agtctcggtc agcggaacga tatcggccac ggctgaagcc
     1081 tcacaagtgg ctattccgtt gagtgccgct attccgcggc gcgcttatgg tggctgttcg
     1141 ccgactcgtc cgcgagccct tcgccactct cggtgaggct ttcgatccgc ggcattgcga
     1201 cgatgttgta gccggcatcc acatagtgga tttcgccggt gacggctgtg gaaagatcgg
     1261 aaagaaggta aagcgctgcg ccgccgacct cgtccaaagc cggtgtccgg ccgagcggag
     1321 aatgctgctt ctgatagttg aaaataacgc gcgcatccga gacgccggag cctgccagcg
     1381 tccgcatcgg tcctggcgat aagccgttga cgcggatgtt ctgctggccg aagtccaccg
     1441 cgagatagcg cacggaggcc tcaagcgcag ccttggctac gcccatcacg ttgtaccgcg
     1501 gcacgacgcg catcgacccg ccataggtca gcgtgatcat cgagccgccg cgcttcatca
     1561 gcgccgccgc acgctttgca atctccgtga acgagaagca cgaaatgacc atcgtgttga
     1621 cgaagttctc gcgggtcgta tcggcgtagc gtcccttgag ctcgttgcgg tcggagaagg
     1681 cgagggcgtg gacgaggaaa tcgatggagc cccactgcga ttgaagttca gaaaaaacgg
     1741 catcgaccga attgaggtcc tgcacatcgc acggaagaat gatcttcgcg ccgaggcctt
     1801 gcgcgagcgg gaccgcgcgc cgtccaaaag catcaccctg atacgtaaaa gcgagttccg
     1861 cgccgtggcc tgcgagcacg cgggctatgc cgtaggcgat cgacctgtca ttggccacgc
     1921 ccataatcag cccgcgttta cccgccatca atggcgacgg ctttgcaata tcgaccacag
     1981 tcatcgatcg aggcctttct aaaccctgcc tgcagctacg ctcttatcgg tcgtagcgct
     2041 tgaagatcaa agtagcgttt gttccaccaa agccaaaact attcgacatc acacagttga
     2101 gttcgacatt atcgagtcgc tcacgcacaa tcggcatatc cgagaacgct gggtcaagct
     2161 cgtctatatt tgcggattca cacacaaatc cgttattcat catcagga
//
LOCUS       DATMGX010000752         2232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_164241, whole genome shotgun sequence.
ACCESSION   DATMGX010000752 DATMGX010000000
VERSION     DATMGX010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164241"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..616
                     /locus_tag="VNR65_12370"
                     /pseudo
     CDS             1..616
                     /locus_tag="VNR65_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
     gene            complement(828..1223)
                     /locus_tag="VNR65_12375"
     CDS             complement(828..1223)
                     /locus_tag="VNR65_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19510.1"
                     /translation="MLKKTVAGATLMAAGLAISAGPALASAAPGAIAIHAQAITDNAV
                     QPVHYRRGGYAVRRPYAGGGWGYRRSVEWGAPYSYGAYNPGYYSGSPGYYGGYSGWGS
                     PAGYWGGYSGWGPGVSIGVAPGFGIGFGF"
     gene            1487..>2232
                     /locus_tag="VNR65_12380"
     CDS             1487..>2232
                     /locus_tag="VNR65_12380"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HWJ19511.1"
                     /translation="MTTNLQNVIDAAFENRQNIKIGQKGEIAEAVEAALALLDSGQAR
                     VAEKKDGEWIVHAWLKKAVLLSFRLNDNRIMGPAVGAPVTGPTSPGPWWDKVPTKFSG
                     WTEDKFRAAGFRAVPGVVVRHSAYIAPGAVLMPSFVNVGAYVGEGTMVDTWATVGSCA
                     QIGRNCHISGGAGIGGVLEPLQANPVIIEDDCFIGARAEVAEGVIVGEGSVLSMGVYL
                     GQSTKIVDRATGEVLYGRVPPYSVVVSGAMP"
BASE COUNT          463 a          672 c          678 g          419 t
ORIGIN      
        1 catgagcaac agttcagaat cggtggcaat cccccgtgag acggcaaccc caggccaaag
       61 aacggcgttg gacaggccgg ggcgttccca cggccctctg aggagggcct tgcgcaatac
      121 ccttcattac ctgtcttatc atttcgtgct gagccggccg aagacgcagg tgtctagggt
      181 tgccggtttc cagcttaccg tgccgccaac cgtcttccat ccaggctact ttatcagcag
      241 tgaggccttc gccaagttcg tcgaccgcct cgacctttcc ggtaagacgg tggtcgacgt
      301 cggaacagga accggcatca ttgcccttgc cgcagcgcgc gcaggtgctg agacggttat
      361 cgctaccgat atcaacccca acgccgccgc tgccgcccag gagaatggca atgcgaacgg
      421 tctcggagag aaggtgaggg gactttgctg caatcttctc gaagcgattg agccgaaacc
      481 ggtgttcgat gtgatcatgt cgagcccgcc gaagcatgcg ggcgagccaa aggatctcgc
      541 cgacgcgggc tggcacgcgg gcgcaggcaa caagaacatc ctcaatcttt tcgagcaggc
      601 gcgacagagg ctgaagccgg gcggccgtgt ctatctcatg atctcttcgg actccgacct
      661 cgacatgtat ggcaaagcca tcgccaaggc cggattcaag gcgcggctcg ctttcgagca
      721 ctcgatcatg ttcgaatctt tcccgctgta cgaactcacg caataactaa aaaaggcccg
      781 ctcccggccg ttaagagcag gggcgggcct tgatcttagg gggaatgcta gaacccgaaa
      841 ccgatcccaa agccaggcgc tacgccgatt gagacgcccg gtccccatcc agaatagcca
      901 ccccaatatc cagccgggga gccccatcca gaataaccac catagtagcc cggagagccg
      961 ctgtagtatc ccggattata ggctccgtaa gagtacggcg caccccattc gacggagcgg
     1021 cgatagcccc agccgcctcc tgcatagggc ctgcggactg cgtaaccacc gcgcctgtaa
     1081 tggactggct ggaccgcgtt gtcagtgatg gcttgggcgt ggatggcgat cgcgcccggg
     1141 gctgcgctcg caagagcagg ccctgcggat attgcgagac ctgcagccat cagggtagcg
     1201 cccgctacgg ttttcttcaa cattaagact ctctttccgt tgtggtttgc gacgctcatg
     1261 gatcacgacc cttcaaggtg aggacacaag gtgagcaatt gagttccagc cacccgaaga
     1321 tgtatcaaat tgctcttaaa ggccgccgtt accgtgagta aaagatcagc gtcggtccat
     1381 ttcgcctgag aactgaggag gctctgcgtt cgatccgacc aaccgacttg acaaaaagcc
     1441 ctgccgaaag gcacgcaatc aagacagcca tagccggagg cagcgcatga caacgaactt
     1501 gcagaacgta atcgatgccg cattcgagaa ccgccagaac atcaagattg gtcagaaagg
     1561 cgagatcgcg gaagcggtgg aggcggcact tgcgcttctc gacagcgggc aggctcgcgt
     1621 cgcagaaaaa aaggacggcg agtggatcgt gcatgcctgg cttaagaagg cggtgctgct
     1681 ttcgttccgg ctgaacgata accgcatcat ggggccagcc gtcggcgccc ccgttaccgg
     1741 gccgacctcg cctggaccgt ggtgggacaa ggttccgacg aaattttcgg gctggacaga
     1801 agacaagttc cgtgcggcgg gctttcgcgc agttccggga gtcgtggtgc ggcattcggc
     1861 ctacatcgcg ccgggggccg tcctcatgcc ctccttcgtc aacgtcggcg cctatgtcgg
     1921 ggaaggcaca atggtcgaca cctgggcgac ggtcgggtcc tgcgcgcaaa tcggaaggaa
     1981 ctgccatatc tcgggcggcg ctggtattgg cggcgtcctg gagccgcttc aggccaatcc
     2041 cgtcatcatc gaggatgatt gcttcatcgg cgcgcgcgcc gaggtcgccg agggcgtgat
     2101 cgtcggcgaa ggctcggttc tgtccatggg cgtctatctc ggccaatcga cgaaaatcgt
     2161 cgatcgcgcc acgggcgagg ttctctatgg tcgcgtgccc ccctattccg tggttgtctc
     2221 cggtgcgatg cc
//
LOCUS       DATMGX010000753         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_227290, whole genome shotgun sequence.
ACCESSION   DATMGX010000753 DATMGX010000000
VERSION     DATMGX010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227290"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1073)
                     /locus_tag="VNR65_12385"
     CDS             complement(228..1073)
                     /locus_tag="VNR65_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ19512.1"
                     /translation="MSSDAIIHCSGVGKAFQLYGHQNDQLKQILFGTWKKFYKEKWVL
                     RDVSFDVAKGERLGIIGRNGAGKTTLLQIICGITQPTKGACDVKGRIAPILALGSAFD
                     GLLTGRENARIGAAILGLNRREIERRMDSIAAFADIGSTFEQPMRTYSSGMVARVAFA
                     ICAHVDADIMIIDEALSVGDQQFQEKCEKFILDFAKTGTILVVSHSLEFLSALCDRIL
                     WLENGVLRMAGDPKTVIKQYSKEMSTNSVNPATVTKSAAKAQRQSLLRAKPIPIRQRK
                     RPKMH"
     gene            1305..1673
                     /locus_tag="VNR65_12390"
     CDS             1305..1673
                     /locus_tag="VNR65_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19513.1"
                     /translation="MRLTGNLALSTAALDYDSRMIGALELSSKKWLFAVQLPGMKKHS
                     RHVVEPNGLALAELIERLKSRSAAAGRVIARGIIPHEAGRDGFWLARFLKRRGVEIHV
                     MQSSILPVDRRAGRAAPRPM"
BASE COUNT          453 a          572 c          612 g          452 t
ORIGIN      
        1 agcgaccaaa tgccgtgaga tggctacatc ggcaatcctc catcgcgtcg aaccattcat
       61 cgaaaagcaa aatgtcggcg gcgacagcag ccgccttcat ggtatccttc gtcatgccgt
      121 ggtctccact ccagcgctgg aatcatttat tgggttagga aacccggaga ctacgccaat
      181 caaattcgac acctccgcga cgggaccatg gcgtcagctc gaaaactcta atgcattttg
      241 gggcgttttc tttgccgaat cggtatcggc tttgcacgca acaggctctg gcgctgcgcc
      301 tttgccgcgc ttttcgtaac cgtggccgga ttgactgaat tcgtgctcat ctccttgcta
      361 tattgcttaa tcacggtttt cggatcgccc gccatccgca gcacgccatt ttcgagccat
      421 aggatgcgat cgcaaagcgc gctcagaaat tcgagactgt gggagaccac caggatcgtc
      481 cccgtcttgg cgaaatcgag gatgaatttc tcgcacttct cttgaaattg ctggtcgcct
      541 acggacaggg cctcgtcgat gatcataatg tccgcatcga catgtgcgca gatcgcgaag
      601 gcgacacgcg cgaccattcc gctggaataa gtgcgcatcg gctgttcaaa ggtcgagccg
      661 atgtcggcga aagcggcgat ggagtccatg cgccgttcaa tttcgcggcg attgaggccg
      721 agaatcgccg cgccgatcct ggcgttctcg cgcccggtca agaggccgtc gaaagcggag
      781 ccgagcgcca ggatcggcgc aatgcggccc ttgacgtcac aggcaccctt cgtcggctgg
      841 gtgatgccgc aaatgatctg caagagcgtc gtcttgccgg caccattgcg cccgatgatg
      901 ccgaggcgtt cgcccttggc gacatcgaag ctgacgtcgc gcagaaccca tttctccttg
      961 taaaatttct tccacgtgcc gaagaggatc tgcttcaact gatcgttttg gtgcccataa
     1021 agctggaacg ccttaccaac gccagaacag tgaatgatgg catcagaaga cattgatcgg
     1081 cacgatttat gcgcgttcaa gaattgcgga ccgcgtgaac atggacaaca aacgaacgag
     1141 tctttccatg gaggatcttc tgcttaaaga gaaaccaacc aagctgccct tagcgcagcc
     1201 aaacggcttt gcacatagat cagttcgacc tggccaatgt caataggtaa tgccgaggat
     1261 tgggtaccct attggtttgt tgctcgaacc gaagggatcc cgatatgcga ctgactggaa
     1321 atctagcgct ttcgaccgcc gctctcgatt acgatagcag gatgatcggc gctttggagt
     1381 tgagttcgaa gaagtggctg ttcgccgtcc agcttccggg catgaagaag cattcgcggc
     1441 atgtggtcga gccgaatggg ctggcgctgg cggagttgat cgaacggctg aagtcgcgtt
     1501 ctgcagccgc cgggcgggtg atcgcgcggg ggatcatacc gcatgaggct gggcgcgacg
     1561 gattttggct tgcgcgtttt ttgaagcggc gcggggtcga gattcatgtg atgcagtcgt
     1621 cgatcctgcc ggtggatcgg cgagcggggc gcgccgcacc aagaccgatg tgatcgacgt
     1681 cgagatcctt tttgcgcacc gattggcatt tgaaccgcga cggaaatttc tgggcgatgg
     1741 atgcggtcgc gcaacagctg ttagcgggca atctcctcca taacggcgta aaaccctaac
     1801 ggtccttccc tggggggcca gggtcgccgc cgctgcggtc cttcgtcgaa tgggccacat
     1861 gatcgaacca acctgcatga tccgcccggg ttaacttaca aaagataacg gacctcgcgc
     1921 agtactcgag cagattgcga ttcctcgact tacaggttaa tgttcggcga tgagagatcg
     1981 ccggggagcg gccaagcatg gtcgccgccc gggtgagccg aaccgatctt ataagaagtc
     2041 caaaacaggt ggattatagc cgcgtggctc gatgtggaat atagagaaa
//
LOCUS       DATMGX010000754         4292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_86537, whole genome shotgun sequence.
ACCESSION   DATMGX010000754 DATMGX010000000
VERSION     DATMGX010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86537"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VNR65_12395"
     CDS             <1..645
                     /locus_tag="VNR65_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWJ19514.1"
                     /translation="ALDVLFILHADHEQNCSSTTMRVVGSSQADPYLSMAGAASALSG
                     PLHGGANEEVLRMLDEIVSVKNIPAYIKRVKAGELKLMGFGHRVYKNYDPRARIIKSV
                     ADEVFEVTGRNTRLDIALELERIALADDYFVTRKLYPNVDFYSGIIYQAMGFQPEMFT
                     VLFAIPRAAGWLAQWNEMIQDPEQKIARPRQIYTGVGQRKIPTTQDEKSAVKVG"
     gene            994..1389
                     /gene="mscL"
                     /locus_tag="VNR65_12400"
     CDS             994..1389
                     /gene="mscL"
                     /locus_tag="VNR65_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWJ19515.1"
                     /translation="MFKGFREFVLRGNVVDLAVGVVIGAAFGTVVTAFVKDLLTPVIA
                     AIAKKPDFSGFFLEVNGAKFLYGDFVNAIIAFLLIALAVYYFVVLPVNALMKRFEKQA
                     LPDKKKCPECESDIPIAARRCAFCSSQIA"
     gene            1786..>4292
                     /locus_tag="VNR65_12405"
     CDS             1786..>4292
                     /locus_tag="VNR65_12405"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HWJ19516.1"
                     /translation="MSPKDSESAAAAAPENNKSLAVPNEKLTQQNGKNGHGKSDGEYT
                     ADSIKVLGGMEAVRKLPAMYIGSTGELGLHHLVYEVVDNSVDEALAGFADHIDVSIHI
                     DNSITVVDNGRGIPTEEMDVDGERLSAAQVVMTVLHAGGKFDTSTYKVSGGLHGVGVS
                     CVNALSHLLELEIWREGSVWEQSYSKGEPITKLKKTGTTKKRGTKVHFLPDKEIFTVS
                     EYNYDTLAQRLREMAFLNKGLLITLTDERTTDQKTGEPKRAEFKYNGGIAEFIKHLNR
                     GKSVLHDKPIYMEAERDDVAMEIALQYNDGYSETVFSFANNINTVDGGSHLSGFRTSL
                     TRTVNYAGQQLGLFKDVKENLTGDDVREGLVAVVSVKLSQPQFEGQTKGKLNSDIAGT
                     VQAFVNERLGAFFEQNPSVAKKIINKAVEAARAREAARKARDLTRRKGALDGGGLPGK
                     LADCSERNPDRCEVYLVEGESAGGTAKQGRDRRFQAILPLKGKILNVEKARYDKMLGH
                     EEIRAMITALGTGIGKDDFDVSKLRYSKIILMTDADVDGSHIRTMLLTFFFRHMQELI
                     KRGNIYIAQPPLYRIKKGKFEQYIKNDAEFVRVMVKRAADGLVVRYGEGAAVLEGAPL
                     TKFMTTLNDFIGFFDKVDKRIRDEKITELLPKADLLKRVDFEGDSKNPSKKIVALEKA
                     LKAIAKDCDIKSVAQRFEEEHSLWECVFVNSQGAEHVINWELASTPEYRQMMSKYKQI
                     ELYMQPPYSIESVKKDAGSGANGKEELSDAERADQEKAEQKSPKTSKRKSEAETVEKA
                     SARELFDYVLNEGRKDYTVQRYKGLGEMSSQQLWETT"
BASE COUNT         1000 a         1189 c         1275 g          828 t
ORIGIN      
        1 gcgctggatg tgctgttcat cctgcacgcc gaccatgagc agaactgcag ctccactacc
       61 atgcgggtgg tgggcagctc gcaggctgat ccctatctct cgatggcagg agcagcgtcg
      121 gcgctctccg gcccgctaca cggcggcgct aacgaagagg tgctgcgcat gttggacgag
      181 atcgtctcgg tgaaaaacat tcctgcctac atcaaacgcg taaaggcagg cgagctcaag
      241 ctgatgggct tcgggcatcg ggtttacaag aactacgatc ctcgcgcacg catcattaag
      301 tctgtcgccg acgaagtatt cgaggtgacc ggacgcaaca ccaggctgga catcgcactg
      361 gaactcgaac gcatcgcgct ggccgacgat tacttcgtca cccgcaagct ctatcctaac
      421 gtcgacttct actcgggtat catttaccag gccatgggct tccagccgga gatgtttacc
      481 gtgctgttcg cgattccgcg agcggccggc tggctggcgc agtggaacga aatgatccag
      541 gacccggagc agaagatcgc gcgtccgcgg cagatctaca ctggagtggg acaacgcaag
      601 attccaacca cacaggatga gaagtcagct gtgaaagtgg gctagttgct ggaaatgtaa
      661 atttgaaaga catggggcgg agcgatccgc ccccattttt tttggcgagc gatcatagtg
      721 tggcgctgga atagcgctgg cggtcctcgc gatctccagc gcagtggcag acagtcgcgt
      781 gtccgacgat cttgcgcgaa ggcgcgcaga tcatgattcg ggtgatggtg attttctatt
      841 tgagccaggg gccagtgaaa gaatggttcg cgaaccacga acttcaactt gcgaatgccg
      901 agcccagcga cgatactaca cagcgcggca gtaacttgaa actagagttg ctggactcgc
      961 gtaaattccg ccgatcctgt tcgggaggag ccaatgttca aaggatttcg ggaattcgtc
     1021 ctgcgcggca acgtggttga tctggcggtg ggcgtcgtga tcggcgccgc gtttggcacg
     1081 gtggtcaccg cgtttgtcaa agatctgctt actccggtca ttgctgctat cgccaagaag
     1141 ccagacttct cgggcttctt ccttgaagtg aatggcgcga agttcctcta cggcgatttt
     1201 gtgaacgcca tcatcgcgtt cctgctgatc gcgctggccg tctactactt cgtggttttg
     1261 ccggtgaatg cgctgatgaa gcgattcgag aagcaagccc tgcccgataa gaaaaaatgc
     1321 cctgaatgcg aaagcgatat acccatcgcc gctcgccgct gcgcgttctg tagctcgcag
     1381 attgcttagc agccgatcga gtggcattgc ccaacgtccg atggactgcg gcgctgccgg
     1441 ggcgaccctt atcttgtcat tgagcttcac ccaaccgggc cgaaaatatt actagagtca
     1501 acttgtatag tgggagtcca atcccccaaa tctgagttgt gctcatcgca tcccaaactc
     1561 tcccatccta cgtctgcaag ctattgaaaa aaggcagttt acggccggta aaagtgatcg
     1621 cggagggagt aattctgtgc tacaatctac acacagcgcc taacccttaa gttgttgact
     1681 tttatagtag ataagggttg tctaagaagg taacggaccc tttgcggcgt ccgggtgtga
     1741 gcaacggaaa cgcacgctcg acgtgtcccg ttggaatgga gcaggatgtc cccgaaagat
     1801 tccgagtcgg ccgccgctgc ggctcccgag aacaacaaat ctctcgcagt tccgaacgaa
     1861 aaattaaccc agcaaaacgg gaagaatggc cacggtaagt cggatggaga gtacaccgcc
     1921 gattcgatca aggtgcttgg cggcatggag gcagtacgca aactcccggc catgtacatc
     1981 ggatcaaccg gcgaactcgg actgcaccac ctggtttacg aagtggtgga caactcggtg
     2041 gacgaagccc tcgcgggatt cgccgaccac atcgatgtct ccattcacat cgacaactcc
     2101 atcacggttg tagataacgg ccgcggcatt ccgacagaag aaatggacgt ggacggcgag
     2161 cgcctgtccg cagctcaagt ggtaatgact gtgctgcacg ccggcggaaa gttcgatacc
     2221 tcgacctaca aagtttctgg cggcctgcac ggggtcggtg tttcctgcgt caatgcgctc
     2281 agtcatctgc tggaacttga gatttggcgc gagggctcgg tttgggagca gagctactcc
     2341 aagggcgagc ccatcacgaa gctgaaaaag accggcacca ccaagaagcg cggtaccaag
     2401 gtccacttcc tgcccgacaa ggaaatcttc accgtatcgg aatacaacta cgacaccctg
     2461 gcccagcgct tgcgcgaaat ggcgtttctg aacaaggggc tgctcattac gctcaccgac
     2521 gagcgcacta ccgaccagaa gaccggcgag cccaagcgcg cggagttcaa atacaacggc
     2581 gggatcgccg agttcatcaa gcacctgaat cgtggcaaga gcgtgctcca cgacaagcct
     2641 atctacatgg aagccgagcg tgacgacgta gccatggaga tcgcgctgca gtacaacgac
     2701 ggctattccg agacggtgtt cagcttcgcc aataacatca acacggttga cggcggtagt
     2761 cacctgtcgg gctttcgcac gtcgctgacg cgcaccgtta actatgcggg ccagcaactc
     2821 ggtttgttca aggacgtgaa agagaacctt acgggcgacg acgttcgcga aggtctggtg
     2881 gcggtggtga gcgtgaaatt gtcgcagccg cagttcgaag gccagaccaa gggaaagctc
     2941 aactccgata tcgccggcac cgtgcaggca ttcgtgaacg agcgcctggg agctttcttc
     3001 gagcagaacc cttcggtcgc caagaaaatc atcaacaagg cggtcgaggc cgctcgtgct
     3061 cgcgaggccg cgcgtaaggc ccgcgatctg acccgccgca aaggagctct cgacggcgga
     3121 ggcttgcccg gcaagctggc cgactgctcc gagcgcaacc ccgatcgctg cgaggtctat
     3181 ctggtcgagg gtgagagcgc aggcggcacc gctaaacaag gacgtgaccg ccgctttcag
     3241 gccattcttc cgctgaaggg caagatcctg aacgtggaaa aggcgcgtta cgacaagatg
     3301 cttggccatg aagaaattcg cgctatgatc acggcgctgg gcacgggcat tggcaaggac
     3361 gatttcgatg tttcgaagtt gcgctacagc aagatcatcc tgatgaccga cgccgacgtg
     3421 gacggctcgc acattcgcac gatgctgctc acgttcttct tccgccacat gcaggaactc
     3481 attaagcgag gcaacattta catcgcgcag ccgccgctgt atcgcattaa gaagggcaag
     3541 ttcgagcagt acatcaagaa cgacgcggag ttcgtgcggg tcatggtgaa acgcgccgcc
     3601 gacggcctgg ttgtccgcta cggcgaaggc gccgcggtgc tggaaggcgc tccgcttacc
     3661 aagttcatga ccacgctgaa cgactttatc ggcttcttcg acaaggtgga taagcgcatt
     3721 cgcgacgaga agattaccga gctattgccg aaggctgact tgttgaagcg cgttgacttc
     3781 gagggtgaca gcaagaatcc ctccaagaag atcgtcgcgt tggaaaaggc gctgaaggcg
     3841 attgccaagg actgcgatat caaatcggtt gcgcagcgct tcgaagaaga gcacagcctg
     3901 tgggagtgcg ttttcgtgaa ctcgcagggc gccgagcacg ttatcaattg ggaactggcg
     3961 tccacgcctg agtatcggca gatgatgtcg aagtacaagc agatcgagct gtacatgcag
     4021 ccgccgtact ccatcgagag cgtgaagaaa gacgctggct ccggcgcgaa tggcaaggaa
     4081 gaactcagcg acgccgagcg cgccgaccag gagaaggctg agcagaagag ccctaagacc
     4141 tccaagcgca agtccgaggc ggaaaccgtg gagaaggcct cggcgcgcga actcttcgac
     4201 tacgtgctca acgagggccg caaggactac accgtgcagc gctacaaggg cctaggagag
     4261 atgagctcgc agcagctttg ggagaccacg at
//
LOCUS       DATMGX010000755         4635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_111593, whole genome shotgun sequence.
ACCESSION   DATMGX010000755 DATMGX010000000
VERSION     DATMGX010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111593"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VNR65_12410"
     CDS             complement(<1..324)
                     /locus_tag="VNR65_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19517.1"
                     /translation="MSLQKRIAFAFILALVAAQPAFADQEKHHALSLVRPPKYPADFK
                     HFDAVNPNAPKGGAVKLSSPAQYDNLNPAVFRGALAAGLATGFALIFDPLMIPSREEG
                     STHYCL"
     gene            complement(431..1024)
                     /locus_tag="VNR65_12415"
     CDS             complement(431..1024)
                     /locus_tag="VNR65_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWJ19518.1"
                     /translation="MKIAYFVGSVLAGMLVLVGLANAIEKFYPKGFENSDKGGQIQVT
                     AVPIARALAAQPEKKAIVTPVSREMEAPDASLSVLLARADPEAGQEKAKTCAICHTWT
                     KGGGVRVGPNLYGVVGRDIARDPGFSYSPALQQKGGRWSFNEIYEWIGDPGELVPGNN
                     MYKMGFAGLKDPQQRANVIAFLAKQSDSPVPLPRSRW"
     gene            complement(1255..1812)
                     /locus_tag="VNR65_12420"
     CDS             complement(1255..1812)
                     /locus_tag="VNR65_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HWJ19519.1"
                     /translation="MNSFYFNKIAGAVLASLLVLVGANTAIHLFYPKGTANLSKGRIV
                     VAPEAAAPASTTPAPAKPEEKDLPVESLLASANVEAGQSVAKACGTCHNWTKGAGAKI
                     GPDLYGVIGREIGKEPGFVYSNALQSKGGKWTFKDLYEWIKDPKAFIPGNKMAFGGIK
                     SSQERANIIAFLNKQSDHPAPLPQK"
     gene            1914..2681
                     /locus_tag="VNR65_12425"
     CDS             1914..2681
                     /locus_tag="VNR65_12425"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HWJ19520.1"
                     /translation="MRFQVAKLFPSKATFNPIVIIPARLGSTRLPRKPLLHISGAPMI
                     VHVWRRAMEADVGPVFVAADSAEIADAVRARGGQAITTVGDHASGSDRIYEALCRIDP
                     EEKFDAVINLQGDLPTLEPRLLGACGAALNRGADIGTLAAVITDPAEINDPNVVKAIA
                     SPLPGGLMNALYFTRASAPHGEGPYYHHIGVYAYRRAALARFVALPPSPLEKRERLEQ
                     LRALEAGMRIHISVVDTVPLGVDTEPDLAKARDILAR"
     gene            2727..3650
                     /locus_tag="VNR65_12430"
     CDS             2727..3650
                     /locus_tag="VNR65_12430"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWJ19521.1"
                     /translation="MAFESNGKSQTTPASVAYQGEPGANSHIACIAYDKRLEPVACAT
                     FEDAISAVRSGAVRCALIPVENSVAGRVADVHHLLPNSGIHVIGEHFERIYHQLMALP
                     GADFSTLKTVHSHTQALGQCRGALQELGLKPVPEADTAGSARMVAEEGDLTKAAIASK
                     LAAEIYGLHILKAGIEDAAHNTTRFLVLANEAEVPPAGNGPVLTTFIFRVRNIPAALY
                     KALGGFATNGVNMTKLESYQLEGSFNASMFYADVEGHPDDRPLRLALEELEFHTAELI
                     ILGTYPASPFRESLKRQSTDDDDVPRARAAC"
     gene            complement(4404..>4635)
                     /locus_tag="VNR65_12435"
     CDS             complement(4404..>4635)
                     /locus_tag="VNR65_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19522.1"
                     /translation="SQPWPFPHSLMIGCWGEAMTDAIELDRTEIADARWFSREETAAM
                     LALAHPDGLFVPPSISMAHTLIRGFVDGLLGN"
BASE COUNT          982 a         1331 c         1311 g         1011 t
ORIGIN      
        1 aaggcaataa tgcgtcgagc cttcctccct cgaagggatc atgaggggat cgaagatgag
       61 cgcgaaaccc gtcgcgaggc ctgctgcaag ggcgcctcga aagacagctg gattcaggtt
      121 gtcgtattga gcgggcgacg aaagcttgac cgctccgccc ttgggagcgt tcgggtttac
      181 cgcatcgaaa tgcttgaaat cagccgggta cttaggtggc cggaccaggg aaagcgcgtg
      241 gtgcttttcc tggtcggcga aagctggctg agccgcgaca agcgccagga taaaggcgaa
      301 ggcaatccgt ttttgaagtg acatgagccg gagggggaac ggtgtttctg atggcgggaa
      361 tttagtcccg cataaagcac cgttccacta aaagatccgt catgctcaca aggttcggtt
      421 aacggctgcg ttaccacctt gatcttggaa gggggaccgg gctgtcgctc tgcttggcca
      481 ggaaagcgat gacgttcgcg cgctgttgcg gatctttcag accggcgaaa cccatcttgt
      541 acatgttatt gcccggcacc aactcgcccg gatcgccgat ccattcatag atttcgttga
      601 aggaccaccg accgcccttc tgctgcaaag cgggcgaata ggagaaacct gggtctcttg
      661 cgatatcacg cccgacaacc ccatagaggt tcgggccaac cctgacgccg ccgcccttag
      721 tccaggtatg gcagatcgcg catgtctttg ccttttcctg cccggcttcc ggatccgctc
      781 tcgccagaag cacgctcagc gatgcgtccg gcgcttccat ttcacggctg accggtgtta
      841 cgatcgcctt tttttcaggc tgcgcggcaa gtgcgcgggc gataggtacg gcagtcacct
      901 ggatctgccc ccctttgtcc gagttttcaa aacctttcgg gtaaaatttt tctatcgcat
      961 tagccagccc aacaagcacc aacattcctg caagcactga cccaacaaaa taggcaatct
     1021 tcattcctgc ctcccatgcc gacgttttgc atcggctttg ttttctggcc gcttgagcag
     1081 cctgttttct tttttgaaat ccgcgtcccc gaggagggac ggcagaatgg aaagcttaag
     1141 ccaaaaaaca tagcttggct atgccaagta ttaagctata tgaataaaaa tcggggtcta
     1201 gcgtaatcct cgcgtaaccg ttcggtaaca gcacaatcca gggagaggag cgcgctattt
     1261 ctgaggaagg ggggctggat gatcgctttg tttgttgaga aaagcgataa tgttggccct
     1321 ctcctgagaa ctcttgatcc ctccaaaggc catcttatta ccgggaataa atgccttcgg
     1381 atccttgatc cactcataaa gatctttgaa ggtccatttt ccgcctttgc tttgcaaggc
     1441 gtttgagtaa acaaatcccg gttccttgcc gatctcccga ccgatgaccc cgtaaaggtc
     1501 tggccctatc ttcgcaccgg cccctttggt ccagttgtga caggtgccgc aggctttcgc
     1561 gacgctctgt ccagcttcta cgttcgcgct ggccaggagg ctctcgacag ggagatcctt
     1621 ttcttcaggc tttgcgggag caggcgttgt cgatgcgggg gcagcggctt caggggcgac
     1681 gacaatgcgg cctttcgaca aattcgccgt gcccttggga taaaaaaggt gaatggctgt
     1741 gttcgcgcca accagcacaa gaaggcttgc cagcacggca cccgctattt tgttaaagta
     1801 gaaggaattc atggtcgctc atgtgctata gtgacgcggc ttagccaccg ccttgatggg
     1861 ctcaaaatct tagggactgc aagacgtcaa cggaaaaacc gtcgacaagt tctatgagat
     1921 ttcaggtggc gaaattgttc ccttcgaaag cgacttttaa cccaattgta atcattccgg
     1981 cgcggcttgg atcgactcgt ctgcctcgta agccgcttct tcacattagc ggcgcgccga
     2041 tgatcgttca tgtctggcgg cgggcgatgg aagcggacgt tggtcccgta ttcgtagccg
     2101 cagactcagc cgaaatcgca gatgctgtga gggcgcgcgg gggtcaggcg ataacgaccg
     2161 tgggcgacca cgccagcggc tccgaccgca tttacgaagc tctctgccgc atcgatcccg
     2221 aggaaaagtt cgatgctgtg atcaacctcc aaggggacct tccaaccctt gagccgcggc
     2281 ttcttggagc atgcggcgcg gcgctgaaca ggggcgcgga cattggcacc ctcgccgccg
     2341 tcatcacgga cccggccgaa atcaatgatc cgaatgtcgt aaaggccatc gcctcgccct
     2401 tgccgggcgg gttgatgaac gcgctctatt tcacgcgcgc atcggcacct catggcgaag
     2461 gcccctatta tcaccatatt ggcgtttacg cctatcgccg cgcagctctc gctcgtttcg
     2521 tggcgctgcc gccgtcgccg ctggaaaagc gcgaacgcct ggagcagctc cgcgcgctcg
     2581 aggctggcat gcgcatccat atttcggtgg ttgacacggt gcctttggga gtagacaccg
     2641 aacccgatct tgcaaaagcg cgcgacatac tcgcaaggtg agcgccgaat ttcctcgccc
     2701 ccaaagtggc gagcttaggg tacaaaatgg cattcgaatc gaacggaaaa tcgcagacaa
     2761 cgcccgcaag cgtcgcctat cagggcgagc ccggcgctaa ttctcacatc gcttgcatag
     2821 cctatgacaa aagacttgag ccggttgcct gtgccacgtt cgaagatgca atttcggcag
     2881 tgcgtagcgg tgctgtgcgc tgtgcgctaa tccctgtgga gaattcggtg gcaggccgag
     2941 tcgccgacgt tcaccacctt ttgccgaatt ccggcattca tgtcatcggc gagcatttcg
     3001 agcgcattta ccatcagctc atggcgctgc ccggcgctga tttctcaacc ctgaagacgg
     3061 tgcacagcca tacgcaggcg ctcggccagt gccggggcgc gctgcaggag cttggtttga
     3121 agcccgttcc cgaggccgac acggccgggt ctgcgcggat ggttgccgag gaaggcgacc
     3181 tgacgaaagc ggcgattgcg tcgaagctcg ccgccgagat ttacggcctt cacattctta
     3241 aagctggcat cgaggatgca gcccacaaca cgacgcgctt cctggtgctc gcgaacgaag
     3301 ccgaggtgcc acccgcgggc aacggcccgg tgctgaccac gttcatcttc cgcgtgcgca
     3361 acattccggc agcgttatac aaggcgctgg gcggcttcgc caccaacggc gtgaacatga
     3421 cgaagctcga gtcctatcag ctggaaggca gcttcaatgc ctcaatgttc tacgccgatg
     3481 tggagggcca ccccgacgac cgcccgttgc ggctcgcgct tgaggaactg gagttccaca
     3541 cggcggagct gattatcctc ggcacctacc cggcgagccc gttccgcgaa agtttgaagc
     3601 ggcaatctac ggacgatgac gacgtgccgc gcgctcgtgc ggcttgctga ggccccaagg
     3661 cgagcttgcg agttacagtg accgcatccg cgagcggatg cggttggaga gaaggaacgc
     3721 ctgcgcgaac cctctcccat tgcgggagag ggtggcgtcc ggctttgcgg ggcgccgggt
     3781 gaggggggtt gcgaaagagg ttcgcaaaat ctgccccgac catcttcgac ggcgcatttc
     3841 cgcaatcaca ttggtaaacc gtttgtcacc ccgtacagag gttaatgcca acatgattta
     3901 caaatgtaag ccggatttat ttcccgccgt gtcttgctta agaagtcgtt tactcagtgc
     3961 gagacagagg agcgccgcgc ctttgaccgg acattagcaa atctatacta agccattgag
     4021 aaaacttgct ttttcatcct taacaaatcg cccattaaat ttaacttaat gccggaattt
     4081 ttccctttat tgggacgcgc taaagtccgt taaatatatt tcaggttcgg ctgttaaccc
     4141 tgctcgcgtc cttggcagcg gaggtagagc aagcgctctt tagcattttc tttgttatat
     4201 caatggcttg agttttgtta acctatcaaa aaagcgcatt gtcctaagat ctccgggtca
     4261 cgcaaccggc gcgaacgcca gcgcaggttg aatccggaga tgaaacagat gccacgatgc
     4321 atacagaaag cagcagcttg gcctttcgag tccgggtgcc catgactgcc gaatcgtctg
     4381 tggccttggc cttgcacacc gtctcaattg cccaaaaggc catcgacaaa gccgcggatc
     4441 agcgtatgcg ccatcgatat ggacggcgga acgaaaagtc catctggatg tgcgagagca
     4501 agcatcgccg ccgtctcctc gcggctgaac cagcgcgcat ccgcaatctc ggttctgtcc
     4561 agctcgattg catcagtcat cgcctcgccc cagcagccga tcatcagcga atgcgggaac
     4621 ggccagggct ggctc
//
LOCUS       DATMGX010000756         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_38243, whole genome shotgun sequence.
ACCESSION   DATMGX010000756 DATMGX010000000
VERSION     DATMGX010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38243"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(404..1444)
                     /locus_tag="VNR65_12440"
     CDS             complement(404..1444)
                     /locus_tag="VNR65_12440"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HWJ19523.1"
                     /translation="MHTTAPTRPLTAADFASSQEVRWCPGCGDYSIIKAVQRVLAEIG
                     SKPENTVFVSGIGCSSRFPYYVETYGYHTIHGRAPAVATGIKLANPDLDVWIVTGDGD
                     GLSIGGNHTAHLLRRNLDCQVLLFNNEIYGLTKGQYSPTSRQGTRSPSTPFGSVEQPA
                     NPSGFALGAGARFVARSVDMAQKELLTLLKRAHENKGTSFVEIYQNCAVFNDGAFDEY
                     ADKKTQPNTVVWVKDGEPMLFSKGTKGLKLNRDELRLEVVDVTDGDWKGAGVLVHDET
                     SRTVAQLLIDMPADGFPLALGVIYCDPMPTFDGAMVALNQKVAASKTRDLQKLLRSGE
                     TWSIGGDTPANQ"
     gene            complement(1458..>2339)
                     /locus_tag="VNR65_12445"
     CDS             complement(1458..>2339)
                     /locus_tag="VNR65_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HWJ19524.1"
                     /translation="EALGLAIMSELPLVVVNCQRGGPSTGLPTKTEQSDLYQAVYGRN
                     GDAPLVVIAARSPADCFESAIEAVRLAVQFMTPVILLTDGYMANAAEPWRLPDLAALE
                     SFAAEFYDQVPSEGRINPYSRDAETLKRIWIKPGTPGLTHRIGGIERSFSTGEISYDP
                     ANHQKMTDTRKAKIDGVANFIPEQDVLLGEPGGKLAVVGWGSTFGAIQQGVQRARANG
                     ADVSHIHIRHLWPLPRNLGTLLKSYERILVPEMNTGQLKTVLRDQYLVDAKSLSKVSG
                     QPFRIAEIEAAIHQALA"
BASE COUNT          460 a          690 c          704 g          485 t
ORIGIN      
        1 ccgtccacgc tcggcgaccc gatgcgacct ctgatctggg tgtcgaagag catggacaag
       61 ctcgctgaga cactaacttg aaaccgggca acgcgaagtg taaattcccg ttctcttcat
      121 tgaccaactc gcacttgtca tggccgcatg acgggcagtt gcgggaaatg cctttggctt
      181 ctagaaatcg aatcgcatct gataatttga tgggcttcat cgttttcgtg ctctcgcgac
      241 cggcgaaggg gggtggccag cagaaatggg ggcccgaccc ctatggctgg ccgtgtcatt
      301 cgaaccgcgg aaccaccctt tacgtgctgg caaagattga tctctgctca cgaaagctca
      361 agcgccgata gccaaagacc acggcaccaa gacgctcagt cattcattgg ttggccgggg
      421 tgtctccgcc aattgaccat gtctctcccg aacggagaag cttctggagg tcgcgtgtct
      481 tgcttgccgc aaccttctgg ttcagggcga ccatggcccc atcgaaggtc ggcatcggat
      541 cgcaatagat aacgccaagg gcgaggggga aaccgtcggc cggcatgtcg ataaggagct
      601 gagcgactgt cctgctcgtt tcatcgtgga caaggacgcc ggcacccttc cagtcgccat
      661 cggtgacgtc caccacctca agccgcaact catcgcgatt gagctttaat cctttcgtgc
      721 ccttggaaaa gagcatcggt tcgccatcct ttacccagac gacggtattg ggctgggttt
      781 tcttgtccgc atattcgtcg aatgcgccgt cgttgaagac cgcgcagttc tgatagattt
      841 cgacgaagct cgtccccttg ttctcatgcg cacgcttaag gagggtgagc aactccttct
      901 gcgccatatc gacggaccgc gcgacaaacc gcgcaccggc gccaagagcg aaaccggacg
      961 ggttcgcggg ctgctcgact gagccgaatg gcgtcgacgg cgagcgcgtg ccttgccggc
     1021 tggtaggcga gtattggccc tttgtcagac cataaatttc gttattgaac aggagcacct
     1081 ggcagtcgag attccggcgc agcaggtgtg ccgtatgatt gccgccaata gacaggccgt
     1141 cgccatcgcc ggtgacgatc cacacatcga gatcgggatt ggcgagtttg atgcccgtgg
     1201 ccaccgccgg cgctcggccg tggatcgtgt ggtacccata ggtttcgaca taatagggga
     1261 agcggctgga acagccgatc cctgacacaa agaccgtgtt ctctggcttc gacccgatct
     1321 ctgccagcac gcgctgaacg gcctttatga tcgaataatc gccgcagccg gggcaccagc
     1381 gcacttcctg gctggaagcg aaatcggcgg ctgtcaatgg gcgggtggga gcggttgtat
     1441 gcatcgaaat cttcctctta cgccagcgcc tgatgaattg ccgcctctat ttcggcgata
     1501 cggaagggct ggccggaaac cttgctcaag gacttggcat cgacgagata ctggtcgcgc
     1561 aggaccgtct tgagctgacc agtattcatt tcgggaacga ggattctttc gtagcttttg
     1621 agaagcgttc ccaggttgcg gggtagtggc cagagatggc ggatgtgaat gtggctgaca
     1681 tcggccccgt tggcgcgcgc gcgttgcacg ccctgctgga tcgcgccgaa cgtggagccc
     1741 cagccaacga cggcgagctt gccgccgggc tctcccagca gaacgtcctg ctcgggaatg
     1801 aagttggcga cgccgtcgat cttggctttt cgcgtgtcgg tcatcttttg atggttagcg
     1861 gggtcgtatg aaatctcgcc cgttgagaag ctccgctcga taccgccgat ccgatgggtc
     1921 agaccgggcg tccctggctt gatccagatg cgtttcaacg tctcggcatc ccgcgaatag
     1981 ggattgatcc gcccttcaga cggcacctgg tcatagaact cggcggcgaa gctttccagc
     2041 gccgccagat ccggaagccg ccatggctcc gcggcgttgg ccatgtagcc atccgtcagc
     2101 aagatgaccg gcgtcatgaa ttgcacagcc aaacgcaccg cctcgatggc cgattcaaaa
     2161 cagtcggccg ggctccgcgc cgcaataacc accagcggag cgtcgccgtt gcgcccataa
     2221 actgcctggt agaggtcgga ttgctcggtc ttggtcggca atccggtgga tggaccgccg
     2281 cgctggcagt tcacgactac gagaggcagc tccgacatga tggcaagccc gagcgcctc
//
LOCUS       DATMGX010000757         3163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_226020, whole genome shotgun sequence.
ACCESSION   DATMGX010000757 DATMGX010000000
VERSION     DATMGX010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226020"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /gene="dnaK"
                     /locus_tag="VNR65_12450"
     CDS             <1..310
                     /gene="dnaK"
                     /locus_tag="VNR65_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWJ19525.1"
                     /translation="EIGDKVSETDRKAVESALAELKTAAAGDDASLISAKAEELTKVA
                     MKLGEALYRQAGGGGADAGGGQAPGGGTTQGGGSAPSDGVVDAEFEEVKDDEGKKKSA
                     "
     gene            462..1601
                     /gene="dnaJ"
                     /locus_tag="VNR65_12455"
     CDS             462..1601
                     /gene="dnaJ"
                     /locus_tag="VNR65_12455"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWJ19526.1"
                     /translation="MKRDYYEVLGLKKGAADHEIKSAFRKLAKEFHPDRNPGSSTAEI
                     NFKEVNEAYEALKDPQKRAAYDQFGHAAFEGPGPNGAAYGFGNDFSASMSEIFDDLFG
                     EFMGGRRTRGRSSKERGSDLKYNMEITLGEAFNGKTAQIRVPASVTCDACGGAGAESG
                     SSPITCPTCQGFGKVRASQGFFTIERTCMTCQGRGEIIQKPCQVCAGSGRVTRERTLS
                     VNIPAGVEDSTRIRLAGEGEAGIRGGPAGDLYIFLSILPDDIFQRDGADLFCRVPITM
                     ATAALGGQVEVPTIDGGRARVKIPEGTETGKQFRLRGKGMPVLRSKLTGDLYVQVEVE
                     TPQNLNRKQKELLKEFEKASTDATHPESANFLSKIKSFWDRAGKD"
     gene            complement(1635..2105)
                     /locus_tag="VNR65_12460"
     CDS             complement(1635..2105)
                     /locus_tag="VNR65_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWJ19527.1"
                     /translation="MLDHRIELLQQVPLFFGLSEDLLSAIADAGQKSFFGAGENLITE
                     EEAGGAAYLIMTGKAGCSRIEGGEALTEDLWPGTLVGELGMLVETVHNVTVTAQERLR
                     ALAFHRDAFRTVMEAHPDLAQHIAERLLVRLHGLAAEIRTVDEKLAQLELEEAA"
     gene            complement(2222..2908)
                     /locus_tag="VNR65_12465"
     CDS             complement(2222..2908)
                     /locus_tag="VNR65_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ19528.1"
                     /translation="MSNTRNILVVDDDQDLALSLAEQLALHEEFETHVAHSAAQAFSA
                     VKGKHTDLVLLDVGLPDMDGREACRLLRKNGFKHPIIMLTGQDSDSDTILGLESGAND
                     YITKPFRFAVLLARIRAQLRQHEHSEEAVFTIGEYSFRPAAKILIDKKGGKVRLTEKE
                     TSILKYLYRAGEKVISRDVLLHEVWGYNAGVTTHTLETHIYRLRQKIERDPSRAELLI
                     TEAGGYKLIP"
     gene            2978..>3163
                     /locus_tag="VNR65_12470"
     CDS             2978..>3163
                     /locus_tag="VNR65_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19529.1"
                     /translation="MRNQNLPQKPQEKTRKRTIARLLTKSDAATTGFLTYKNLHFSAA
                     LGKSGTRAGKKEGDGATP"
BASE COUNT          726 a          872 c          944 g          621 t
ORIGIN      
        1 ggaaattggc gacaaggtat ctgaaacgga ccgcaaggcc gtcgaatccg ctctggcgga
       61 actcaagacc gccgcagctg gggatgatgc gtcgctgatc agcgcaaaag cagaagaact
      121 cacgaaggtc gccatgaagc tcggagaggc actctatcga caggctggcg gcggcggtgc
      181 tgatgctggc ggcggccagg ctccaggtgg tgggacgact caaggcggtg gttctgcgcc
      241 gtccgatggc gttgttgatg ccgagttcga ggaagtcaag gacgacgaag gcaagaagaa
      301 gtcggcgtag cttcatggtt tggggagagc gcggtgctct cccattttgc cgcgcgggct
      361 agccagcagg ctcagcgtgc aatatacgta tactccaaaa gcttaagctg atctttttaa
      421 ttgccacgaa gcttcgatga tcgaggatct caaaacagac catgaagcgc gactattatg
      481 aagtgctcgg actgaagaag ggcgcagccg atcacgaaat caaaagcgct ttccggaagc
      541 ttgcaaaaga attccacccg gatcgtaacc caggaagcag cacggccgag atcaacttca
      601 aggaagtgaa cgaggcctat gaggctttga aggaccccca aaagcgggcc gcttacgatc
      661 agttcgggca tgccgcattc gaaggtcccg gtccaaatgg cgcggcctac gggttcggaa
      721 atgatttctc ggcctccatg tccgagatct tcgatgatct cttcggcgag ttcatgggcg
      781 gccgccgcac tcgcggacgg agttcgaagg agcgcggctc cgatctcaaa tacaacatgg
      841 agatcactct cggggaggcc ttcaacggaa agactgcgca aatccgcgtt ccagcttccg
      901 taacctgcga tgcttgcggt ggcgcgggcg cggaatcagg atcgagcccc atcacctgcc
      961 cgacctgcca aggcttcggc aaagtccgcg cgagccaggg cttcttcacg attgagcgta
     1021 cctgcatgac ctgccagggc cgcggtgaga tcattcagaa gccctgccaa gtctgcgcag
     1081 gcagcggtcg agttacacgc gaacgaacct tgtcggttaa catcccggcg ggtgtcgaag
     1141 attcgacgcg gatcagattg gcgggcgaag gcgaggccgg gattcgcggc ggccctgcgg
     1201 gcgatcttta catcttcctc tcaatcctgc cagatgacat cttccagagg gacggcgcag
     1261 acctgttttg ccgggtcccg atcacgatgg caactgcggc gctgggcgga caggtcgagg
     1321 ttcccaccat cgacggcggt cgcgcgcggg tgaaaatccc tgaaggcacg gaaaccggga
     1381 agcagtttcg tttgcgcggg aaaggcatgc cggtgcttcg gtcgaagttg acgggcgatc
     1441 tttacgttca agtcgaagtc gagacgcccc agaacctgaa ccgcaagcaa aaagagcttc
     1501 tcaaggagtt cgaaaaggcg tccaccgatg cgacgcatcc cgagagcgca aacttcttga
     1561 gcaagattaa gtcgttctgg gaccgagcgg ggaaagactg aggctttagg cgcgattggc
     1621 ggccttctac aggcttaggc agcctcttcc agttccagct gcgccagctt ctcatccacc
     1681 gtacgaattt ccgccgcgag cccgtgaagg cgcacgagaa gcctctcggc aatgtgttgc
     1741 gccaagtccg ggtgcgcttc catgacagta cgaaatgcat cgcgatgaaa ggcaagcgcg
     1801 cgaagccgtt cttgcgccgt gacggtgaca ttgtggacgg tttcgaccag cattccaagc
     1861 tcgcccacga gcgtgccagg ccaaagatcc tctgttaggg cttcgccgcc ctcgattctt
     1921 gagcacccgg ccttaccggt catgataaga taggcggcgc cgccagcttc ttcttcagtg
     1981 atcagattct caccggctcc gaagaaagac ttctggccag cgtcggcgat tgcgctcagc
     2041 agatcctccg acagtccaaa gaacaaagga acctgctgca aaagctcgat tctgtggtcg
     2101 agcatcggaa tcaccttccc ggttgttagc gcatatttaa ctcctcacaa caacgagcat
     2161 agatttgttt gattcgaaag atttcgcaac gcagagtggg ccacacctct tggcgaaagc
     2221 gtcaggggat gagcttatat ccgcctgcct cggtgatgag gagctcggcg cgggatgggt
     2281 cgcgctcgat tttctgacgt agacgataga tgtgggtttc gagcgtatgg gtcgtgacgc
     2341 cagcgttata tccccacact tcatgcagga gcacgtcacg cgaaatgacc ttttcgcccg
     2401 ccctgtagag atatttaagg atcgacgtct ccttctcggt aaggcgaact ttgccaccct
     2461 ttttatcgat cagaattttg gccgcggggc ggaatgaata ttccccgatc gtgaagacgg
     2521 cttcctcgct atgctcgtgc tggcgcagct gagcgcgaat gcgagccagc aaaaccgcaa
     2581 accggaaggg cttggtgata tagtcgtttg cgcctgactc caggccgagg atggtgtcgg
     2641 agtcgctatc ctggccggtg agcatgatga tggggtgctt gaaaccgttc ttgcgcaaaa
     2701 ggcggcaggc ttcgcgccca tccatatcgg gaagtccgac atcgagaaga acgagatcag
     2761 tatgcttgcc cttcacggcg gaaaaggcct gagcagcgct gtgagccacg tgcgtctcaa
     2821 actcttcatg gagagcgagt tgctccgcca gcgacagcgc aaggtcttga tcatcgtcga
     2881 caaccaggat attgcgcgta ttgctcataa ttggctccga tcaaagctta cgcttctgat
     2941 ttaagaggcg gggctgcgca acgcaacggg tcacctcgtg cgcaatcaaa atctacctca
     3001 aaaaccgcaa gaaaaaacac gaaaacgaac catcgcacgg ctactcacca agtcggacgc
     3061 ggccacgacc ggtttcctga catataaaaa tctacatttt tccgcggctc ttggaaagag
     3121 tggaacgcgg gccgggaaaa aggaggggga cggagcaacg ccg
//
LOCUS       DATMGX010000758         2508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_385220, whole genome shotgun sequence.
ACCESSION   DATMGX010000758 DATMGX010000000
VERSION     DATMGX010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385220"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..479)
                     /locus_tag="VNR65_12475"
     CDS             complement(18..479)
                     /locus_tag="VNR65_12475"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HWJ19530.1"
                     /translation="MSCLTGIIANLCSPLRTGACAGFPQTSRSVTLSREPYNALDPPG
                     SLALCALVWSLAAAGIVLLLAFPRCFARVSLALYLGISWIVLGVIRTLIDRLPIEAVA
                     LLVGGGVVYSVGALIHTRERWRFHTPVWHALVLLAAGLHLTALYAAFLSVT"
     gene            457..879
                     /locus_tag="VNR65_12480"
     CDS             457..879
                     /locus_tag="VNR65_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ19531.1"
                     /translation="MIPVRQDKSARDLRRAAQSERGGKIVRRLLGIAHVLDGRGREES
                     ATLVGLAPQSLARAIKRYNAEGIAGLKDRIIPGRPPRLTPEQAVELKQIMLARRDGRQ
                     GDHGKFTVREVVELIEQKWGARISAEAVRTKLHSMHLK"
     gene            968..2035
                     /locus_tag="VNR65_12485"
     CDS             968..2035
                     /locus_tag="VNR65_12485"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWJ19532.1"
                     /translation="MIAVRQDQGAQGLRLAARLEKMGKVVLRLLGIAHILDGRGRAES
                     AALVGLEPQSLARAVDRYNAEGIAGLKDRTIPGRPCKLSSEQSAELKRIVLAGPEDRE
                     NGHPEFKIRHIVDLIEKKWGIRMSAEAMRTKLHSLKLEKLVCRPVHHKTDPVAQAEFK
                     ATFPERLAKIAKDHPEADSLEVWVQDEVRVGQKGKVVRRWAEKGSSPRAVVHGGFKSA
                     WLFGAFCPARDTAVAIVVEAVNAEAMSAHLAVLSQAVPPKVHAVVLVDRAGFHTVAKD
                     LIVPSNMTLVDLPAYSPELNPAEGVWEFLKGGVLAHRLYRTVDEIIDKLCWAWQSLIN
                     EPGRIRSLCSYDWLMVPPAPT"
     gene            complement(2274..>2508)
                     /locus_tag="VNR65_12490"
     CDS             complement(2274..>2508)
                     /locus_tag="VNR65_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HWJ19533.1"
                     /translation="LFAFITMNWRAKPLVSYLVIIQLIANTTTDTGLTVTCQIDANTY
                     TKGIKVSDAEMASLNIKPATFHGEWNYTVPGKF"
BASE COUNT          521 a          693 c          840 g          454 t
ORIGIN      
        1 agagcggcca atctccgtta cgtgacagat aggaacgccg cgtaaagagc tgtcagatgc
       61 aggcctgcag ccaggagcac gagagcgtgc cagacggggg tatgaaaccg ccagcgctca
      121 cgcgtatgga tcagcgcgcc aaccgaataa acaaccccgc caccaaccag cagcgcgacc
      181 gcctcgattg gcagccggtc aatcagcgtg cggatgacgc cgaggacgat ccagcttatg
      241 cccaggtaga gtgccagcga aacgcgcgcg aagcaccgag ggaaggcgag cagcaggacg
      301 atcccggccg ccgccagcga ccacaccagc gcacacagcg ccaggctccc gggcggatca
      361 agcgcgttat acggttcccg cgaaagagtg acggatctgg atgtttgcgg gaaaccggcg
      421 cacgctccgg tccggagagg cgagcagagg ttggcgatga tcccggtgag gcaggacaag
      481 agcgcccggg acctgcgtcg ggcggcacag tctgaaaggg gcgggaagat cgttcgccgc
      541 ttgcttggga ttgcgcatgt gctggatggg cggggacgcg aagagtcggc cacgctggta
      601 ggtctggcgc cgcagagcct ggcgagagcg ataaagcggt acaacgccga gggaattgcc
      661 gggctgaagg accggatcat ccccgggcgt ccgcccaggc tgactccgga gcaagctgtc
      721 gagttgaagc agatcatgct ggcgcggcgt gatggacggc aaggtgacca tggcaagttc
      781 acggttcgtg aagtcgtgga gttgatcgag cagaaatggg gagctcgcat cagtgccgaa
      841 gcggtacgca cgaagctgca ttcgatgcat ctgaaatagc gctgcctatc gaaacggggg
      901 aatcgaattt ggatatttcc aggaagtgtg cgcacgctcg gattggataa tcgagcggag
      961 gctgacaatg attgcggtgc ggcaggacca gggtgcccag ggtctgcgtt tggcagctcg
     1021 gcttgagaag atggggaagg tggttctccg cttgcttggg attgcgcaca tcctggatgg
     1081 gcggggacgc gcagagtcgg ccgcactggt cggtttggag ccgcagagcc tggcgagagc
     1141 ggtggatcgg tacaacgccg agggaatcgc tgggctgaag gaccggacca tccccgggcg
     1201 accatgcaag ctgtcttcgg aacaaagcgc cgaattgaag cggatcgtgt tggccgggcc
     1261 cgaagaccgg gaaaacggac atccggaatt caagattcgc cacatcgtcg atctgatcga
     1321 gaagaaatgg ggcattcgca tgagtgccga ggccatgcgc acgaagcttc attcgctgaa
     1381 gttggagaaa ctggtgtgcc gaccggtgca ccacaagacg gatccggtcg cccaggccga
     1441 gttcaaggcg acgttccctg agcgccttgc caagattgcg aaggaccacc ctgaggcgga
     1501 cagtctcgaa gtgtgggtgc aggatgaagt gcgggtcggc cagaagggga aagtggtgag
     1561 gcgctgggcc gagaagggca gcagtccgcg ggccgtcgtg catggcggct tcaaatcagc
     1621 ctggttgttc ggggcgttct gccccgcacg cgacactgcg gtggcgatcg tcgttgaggc
     1681 ggtaaacgca gaggcgatga gtgcgcacct tgccgtgctc tcccaggcgg ttccgccgaa
     1741 agtccatgcc gtcgttctgg tcgatcgcgc aggctttcac accgtggcga aggatctcat
     1801 tgtgccgtcc aacatgacgc tggttgacct gcctgcgtac agcccggaac tgaaccccgc
     1861 tgaaggggta tgggaatttc tcaaaggcgg agtccttgct catcggctct atcgcaccgt
     1921 ggacgagatc atcgataagc tttgctgggc ttggcagtcc ctcatcaatg aacctggccg
     1981 cattcgcagc ctctgctcct atgactggtt gatggtcccg cccgcgccga cgtaaccgtc
     2041 ctcgccgccc aagcacaccg cgctctctca cgacctacat tttacgccgc aaacagccgc
     2101 ggcgtgcttt cgcatgccca ttttgtgaga tcatttcgtt aaccgtctgt cggttgtcct
     2161 ccatgcttcc gccatctggc tccttcatca ccggattccg ctctcttgtc accggaaacg
     2221 atgcctcaca ggaggaactc gactcgggcg gtcagaaccg ggccggaaaa ttattaaaat
     2281 ttcccgggaa ccgtataatt ccactcgcca tgaaaggtgg cgggtttgat attgagggag
     2341 gccatctcgg cgtcggagac cttgatgccc ttcgtgtagg tgttggcgtc gatctggcag
     2401 gtgacggtaa gtccggtgtc ggtggtggtg ttggcgatca actggatgat gaccaggtag
     2461 ctgaccaacg gcttcgcacg ccagttcatg gtgatgaagg cgaacagc
//
LOCUS       DATMGX010000759         2186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_295766, whole genome shotgun sequence.
ACCESSION   DATMGX010000759 DATMGX010000000
VERSION     DATMGX010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295766"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1041)
                     /locus_tag="VNR65_12495"
     CDS             complement(100..1041)
                     /locus_tag="VNR65_12495"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ19534.1"
                     /translation="MNISLRLLRYVTAAADCANVTEAARRLQVSQPSVSAAIAELEQM
                     LGITIFVRHHAKGVTLTPAGQRLIAEARLLLKHAEEFSKTAEALGDTDRGEITVGCFP
                     TLASLYMPSIMLEFAESHPLITLRLNEGDQEQLMRDLAQGRIEMAFSYIHGVPPGFDT
                     KPLLSLPAVVSLPADHRLAKAPDVSLTELEKEPLIYFDTPLIRDWVASVFDALSIQPN
                     VRLRIRSYELMRGLVARGHGYAVHASVPLTNTTYDGGQVVVKKLRDKVPAAQVVSVTP
                     GSHLMRPAVKIFEDFLEDSLQGRRGDKRSILKSTAAA"
     gene            complement(1255..1428)
                     /locus_tag="VNR65_12500"
     CDS             complement(1255..1428)
                     /locus_tag="VNR65_12500"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWJ19535.1"
                     /translation="MDKDRIKGSAQQAKGSIKEAVGKATGDSKTQAQGKADKASGKAR
                     NTVGGIKDALKGK"
     gene            complement(1528..>2186)
                     /locus_tag="VNR65_12505"
     CDS             complement(1528..>2186)
                     /locus_tag="VNR65_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ19536.1"
                     /translation="RGHGRSSQPWTGNDMDTYADDLAALTEALNVRDAIHVGHSTGGG
                     EVARYIGRHGTRRVAKAALIGAVPPLMLKTEANPSGTPIEAFDQLRASVVSDRSKFWK
                     DLSLPFYGYNRPGAKVSEGVRESFWLQSMMAGFPASYFCIKAFSETDLTDDLKKFDVP
                     TLILHGDDDQIVPIQASALLSSKLIKGAHLKVYPGAPHGMCTTLKNEVNAELLGFIQG
                     "
BASE COUNT          463 a          614 c          632 g          477 t
ORIGIN      
        1 ttatcgccta gagcagcccg catccactcg ggcgcgggtg agctgctctc tagcgcttta
       61 gaaccgatca actctcccgc cttaaatcgg cgggttgatc tacgcggcag ccgtcgattt
      121 gagaatggac cttttgtcgc cgcggcgtcc ctggagcgaa tcctcgagga aatcctcaaa
      181 gattttcacc gcggggcgca taagatgcga cccgggtgtg acactcacga cctgagccgc
      241 aggcacttta tcccgcaact tcttcactac gacctggccg ccgtcatacg tcgtgttggt
      301 gagcggcacc gaggcgtgga cggcatagcc gtgaccgcgg gcgacaaggc cgcgcatcag
      361 ctcgtaagat cttattctca gccggacatt cggctggatg gaaagcgcat caaagacgct
      421 cgcgacccaa tcgcgtatca gcggtgtgtc gaaatagatc agcggctcct tttcgagctc
      481 cgtcaagctc acgtcaggcg ctttggccaa acgatgatcc gcaggcaagg aaacaacggc
      541 aggaagggat aaaagcggct tcgtatcgaa gcctggcggg acgccgtgaa tataggagaa
      601 ggccatttcg atccggcctt gggccagatc ccgcatcagc tgctcctggt cgccctcgtt
      661 gagccgcaag gtgatgagtg ggtggctctc ggcaaattcg agcatgatgg agggcatgta
      721 gagagacgcg agggtcggaa agcatccgac cgttatctca ccgcgatcag tgtccccgag
      781 cgcctcggcc gttttcgaga actcctcggc gtgtttcaaa agcagccttg cctctgcaat
      841 cagccgctgc ccggcggggg tgagcgtcac cccttttgca tgatggcgta caaatatggt
      901 gataccgagc atctgctcca attcagcaat ggctgcggag actgacggct gggatacctg
      961 gaggcggcgg gcggcttccg tgacattagc gcagtcggcg gcagccgtga cgtatcttaa
     1021 aaggcgtaaa gagatgttca tgtgccaatg cttagcctaa gtcgtggctt ataggccaca
     1081 aaagaaagtc taatactttc gttgccaaac gatcgtatac aaattaccgt gagcacaaat
     1141 ttagcttagc aaccttatgc gagaccgaaa tggttgcaca ctagtgtgat atggaaatat
     1201 ttatgtgcaa taagtaaaaa atgcccccgg cttagcgggg gcattcaggc tatcttactt
     1261 gccctttaag gcatccttaa tgccgccgac tgtgtttcgt gctttcccgg aagccttgtc
     1321 ggccttccct tgggcttgtg tcttggaatc gcccgttgcc ttgccaactg cttccttaat
     1381 cgagcccttg gcctgctggg cggaaccctt gatgcgatct ttgtccatcg tcaaactcct
     1441 gtgcgttagc tgaatggtaa ctctcgaaac ggcggagagt tgccaaagat gagttccgcg
     1501 acttcgacct tatttgagcc gcgaggctca accctggatg aagccgagca actcggcatt
     1561 gacctcattc ttaagagtgg tgcacatgcc atgcggagcg cccggataaa cttttagatg
     1621 cgcgcccttg ataagttttg aggacagaag cgcggaggcc tggatcggaa cgatctgatc
     1681 atcgtcgcca tgcaggatga gggtcggcac gtcgaatttt ttcagatcgt cggtcagatc
     1741 ggtctcggag aacgccttga tgcagaagta agacgcgggg aacccggcca tcatgctctg
     1801 cagccagaac gattcacgca cgccttccga cactttcgca cccggtctgt tgtagccgta
     1861 gaagggaagg ctgaggtctt tccagaactt ggatcggtcg gaaacgaccg aagcgcggag
     1921 ctgatcgaat gcctctatcg gcgtaccgct cggattcgcc tcggttttca gcatcagcgg
     1981 cggcacagcg cctatgagcg ccgccttggc gacgcgcctt gtcccgtgcc ggccgatata
     2041 gcgagcaacc tcgccgccgc cggtggaatg ccctacatgg atcgcatccc gcacattgag
     2101 cgcttctgtc aaggccgcaa gatcgtccgc gtaggtgtcc atgtcattgc cggtccaggg
     2161 ctggctagag cggccatggc cgcgcc
//
LOCUS       DATMGX010000760         2237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_446013, whole genome shotgun sequence.
ACCESSION   DATMGX010000760 DATMGX010000000
VERSION     DATMGX010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446013"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VNR65_12510"
     CDS             <1..867
                     /locus_tag="VNR65_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513337.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HWJ19537.1"
                     /translation="ALEKLRLNDASFFFEPESSVALGFGFRCGFLGLLHMEIIQERLE
                     REFNLDLITTAPGVRYKIYKTDGKMVEIDNPSRWLDPSEIDKIEEPVILATILTNEEY
                     VGGILALVEEKRGKQKTFEYVSSSRVMLHYELPLNEIVLDFYDRLKSVSRGYASLDYH
                     LADYWESPMVKLDILVAGEPVDALSIIVHRDFAYERGKALVSKMRELIPRQMFEVPIQ
                     ASIGAKIIARETVHAMRKNVIAKCYGGDISRKRKLLEKQKEGKKRMKRIGRVDIPQEA
                     FLAVLKVGESSN"
     gene            890..1327
                     /locus_tag="VNR65_12515"
     CDS             890..1327
                     /locus_tag="VNR65_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19538.1"
                     /translation="MNQYPLAFIPLIFLSWCAVSFLIAAVSGWMDLAQRLRLSSPFAG
                     STWSFQSASMRWMSRYRSCLNVGADPSGLQLSMFFLFRPGHPPLFIPWSEVTVARRWT
                     FLFVPQVKLLLGREEQIPFVISETLADRIQAAAGASWPIEQVS"
     gene            1413..>2237
                     /locus_tag="VNR65_12520"
     CDS             1413..>2237
                     /locus_tag="VNR65_12520"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ19539.1"
                     /translation="MQKLLLALLLLSASMTVAQSDAPTFTAELFRYEVHSNIVYHTAN
                     NYEAKLDVYQPAGKSQPTPVVIVIHGGGWIAGTKEERVLEIMPYLQMGFAAVNVEYRM
                     AQVSLAPAAVEDCRCALHWVFANAKKYNFDPNRVVLQGGSAGGHLALTTGMLTPAAGF
                     DHECYTGEDNVWSQNPGTDKDPRVAAIVNWFGIADVLDELHGPNAKGYAVAWLGDQPN
                     ADELAKRLSPIHYVDKNTPPIITIHGDKDALVPYEQSVRLHKALDAAGVPNQLYTVP"
BASE COUNT          468 a          731 c          575 g          463 t
ORIGIN      
        1 gctctcgaaa aactccgcct caacgacgcc agctttttct tcgagcccga aagctccgtc
       61 gcactcggct tcggttttcg ctgcggcttc ctcggcctgc tccacatgga gatcattcag
      121 gagcgtctcg agcgcgaatt caatctcgac ctcatcacca ccgcccccgg cgtccgctac
      181 aagatctata aaaccgacgg caagatggtc gagatcgaca atccttcgcg ctggctcgat
      241 cccagtgaaa tcgacaagat cgaagagccc gtcatcctcg ccactatcct caccaacgag
      301 gaatatgtcg gcggcatcct cgccctcgtc gaagaaaaac gcggcaagca gaaaaccttc
      361 gagtacgtaa gctcctcgcg cgtcatgctc cactacgaat tgccgctgaa cgaaatcgtc
      421 ctcgactttt acgaccgcct caaatctgtc tcccgcggct acgcttccct cgactaccat
      481 ctcgccgact actgggaatc gcccatggtc aagctcgaca tcctcgtcgc cggagagccc
      541 gtcgacgccc tctccatcat cgtgcaccgc gacttcgcct acgagcgcgg caaggctctg
      601 gtttccaaaa tgcgcgaact cattccccgc cagatgttcg aggtccccat ccaggcctcg
      661 atcggcgcca agatcatcgc ccgcgaaacc gttcacgcca tgcgcaaaaa cgtcatcgcc
      721 aaatgctacg gcggcgacat ttcccggaag cgcaagctgc tcgagaaaca gaaggaaggc
      781 aaaaagcgca tgaagcgcat cggccgcgtc gacatcccgc aggaagcttt cctagctgtg
      841 ctcaaggttg gcgagagcag caactagagg aaatctattg caaggtttct tgaaccaata
      901 tcctctcgca ttcattccgc tgatcttttt gtcctggtgc gccgtttcat ttctgatcgc
      961 ggccgtcagc ggctggatgg atttggctca acgacttcgc ctctcgtcgc ccttcgcagg
     1021 atcaacctgg agtttccaga gtgcaagcat gcgttggatg tcccgctatc gcagctgcct
     1081 caatgtcggc gccgatccaa gcggtcttca gctgtccatg ttctttcttt ttcgcccagg
     1141 ccacccgccc ctgttcattc cctggtccga ggttaccgtc gcgaggcgat ggacgttctt
     1201 gtttgtcccg caagtcaaat tgctgttggg ccgcgaggag cagataccgt tcgtgatcag
     1261 cgaaactctg gccgaccgca ttcaggcggc tgccggagca agttggccca ttgagcaagt
     1321 ctcttagctc ctgcccatat cctcacttag aggagtatca ttccgtttcg cgccgtacgc
     1381 cgacgcaaaa ctgaattcat ttcaaaggac ctgtgcaaaa actcctgctt gccctcctgc
     1441 tgctttctgc atcgatgacc gtagcgcaat ccgacgcgcc gacattcacc gccgagttgt
     1501 tccgttacga agtgcattcc aacatcgtct atcacactgc gaataactat gaggccaagc
     1561 tcgacgtcta tcaacccgcc ggcaagtcac agcccacgcc cgtcgtcatc gtgatccatg
     1621 gcggcggttg gatcgcgggc acgaaggaag agcgcgtcct cgaaatcatg ccctacctcc
     1681 agatgggatt cgctgccgtg aatgtcgaat accgtatggc gcaggtgtcg ctggctccgg
     1741 ccgcggtgga agattgccgc tgcgcgctgc attgggtatt tgccaacgcg aagaagtaca
     1801 acttcgatcc caaccgcgtg gtccttcagg gcggatcggc cggaggtcac ctggcgctca
     1861 ccactggcat gctcacgccc gcggcgggat ttgaccacga gtgctacacg ggtgaggaca
     1921 acgtctggag ccagaatccc ggcacagaca aagatccccg cgtcgccgcg atcgtgaact
     1981 ggttcggcat cgccgatgtt ctggacgagt tgcacggacc gaacgccaag ggatacgcag
     2041 tcgcatggct gggcgatcaa ccgaatgctg acgagcttgc gaagcgtctc tcgcccatcc
     2101 actacgtcga caagaacacg ccgcccatca tcacgattca cggtgacaag gacgcgctcg
     2161 ttccctacga gcagtccgtg cgcctgcaca aagcgctcga cgccgccggc gtgcccaatc
     2221 agctctatac ggttccc
//
LOCUS       DATMGX010000761         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_102707, whole genome shotgun sequence.
ACCESSION   DATMGX010000761 DATMGX010000000
VERSION     DATMGX010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102707"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1124)
                     /locus_tag="VNR65_12525"
     CDS             complement(63..1124)
                     /locus_tag="VNR65_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWJ19540.1"
                     /translation="MESIAADLAQRLAACAEAVCHHYLPNGVRRGSYWIVGDVSGAKG
                     RSLYVRLRSSFSAAAGNWTDAESGQYGDLLDLIRLNRDLPTLREAFEEARMFLNEPPQ
                     ARRGTCVSTAETSDRRKPSEAAARLFALTSPIPGTTAETYLRGRGMTGDLDDPALGFL
                     ANCYLRAEGSIGPKPLPALIARVTDLNGRITGVHRTWLRADGSGKADLPEPRKALGEL
                     HGHGVRFGIPSAVLAAGEGIETMLALRSVLLHLPSVAALSAAHLGALRLPDGLKRLYI
                     ACDNDRAGLDAGLALASRAVSQGIAVRLLVPAGKDFNDDLKSLGIASLVQRLGPHLWR
                     DDQGILLPRLRLGAESAGP"
     gene            complement(1161..>2805)
                     /locus_tag="VNR65_12530"
     CDS             complement(1161..>2805)
                     /locus_tag="VNR65_12530"
                     /inference="COORDINATES: protein motif:HMM:NF025240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="strawberry notch C-terminal domain-containing
                     protein"
                     /protein_id="HWJ19541.1"
                     /translation="IDKLASLPAVPSGLDQIVQHFGTDQVAEVTGRSRRIVRKLDSQG
                     QPILQVENRPSMANLSETQAFMDDDKRILVFSDAGGTGRSYHADRGVKNQRKRIHYLL
                     EPGWRADAAIQGLGRSNRTNQAQPPLFRPVATDVRGEKRFLSTIAKRLDSLGAITRGQ
                     RQTGGQGLFRPGDNLESVYARDALRHLYDLIYRGKCSCLTLEAFEEATGLSLCDGDGT
                     LREELPPITTFLNRLLALPIDIQNKLFEEFETLLTVRIEGAIAAGLYDQGLETLTGEK
                     LIVSVRETLAHHGDSGAETCLLTIEQHEKLNPIALESILGSRKHGDRLLVNERTGRAA
                     LATSAPSLLGEAGEVIERLRLQRPLSRESMALADLEHSFWNEADAGAFAAAWAEELKS
                     LPETRVNVLHLVTGLLLPVWQKLPRKGCRIFRLTTTEGERLIGRLIRHDDLAELRNAF
                     GLDAKDGALSPEVAIDLLLSEARPIRLHDGLTLRKAKVMGRDRVELTGFGESSVPALK
                     ALGLVSEIIAWRLRLFVPLGEGCAAIFSRLFEHSPPTAH"
BASE COUNT          528 a          853 c          920 g          504 t
ORIGIN      
        1 ctgcgcgccc tcttactgtc ctcgaagctg agacccgcct tccgcgaaga gcgtttctct
       61 gttcaaggcc cagcgctttc cgcaccaagc cggaggcgag ggagcaaaat gccctggtca
      121 tcacgccaga gatgcggccc gagccgctgc accagcgatg caatgccgag ggacttcaga
      181 tcatcgttga agtctttccc cgccggcacg agcagccgga ctgcgatgcc ttgcgaaaca
      241 gcccggctcg cgagcgcaag tcctgcgtca aggcccgcgc ggtcgttatc gcaggcgatg
      301 taaagacgct tgaggccgtc tggcaggcgc agcgcgccaa ggtgcgcggc ggacaacgct
      361 gcaacactcg gaaggtgtag aagaacggag cgcaacgcca gcatggtctc gatgccttcg
      421 cccgcggcga gaacggcgct cgggatgccg aagcggactc catggccatg caattcgcca
      481 agcgcttttc ttggctccgg caaatccgcc ttgccgcttc cgtctgcgcg aagccaggtg
      541 cgatgcacgc cggtgatgcg gccgttaaga tcggtcacgc gggcaatgag cgcaggcaga
      601 ggcttgggcc cgatgctgcc ctcggcgcgg agatagcagt tggccaggaa gccgagggct
      661 gggtcgtcga gatcgccggt catgccgcgg ccccgcaggt aggtctcggc tgtcgtgcca
      721 ggaatggggc tcgtcagcgc aaacaggcgt gcggcggcct cggagggctt gcgcctgtcg
      781 ctcgtttccg cagtggaaac acaggttccg cgacgagcct gagggggctc attcaggaac
      841 atgcgggcct cttcaaaggc ctccctcagg gtaggaaggt cgcgattgag acggatgagg
      901 tcgagcaggt cgccatattg tcccgattcg gcatccgtcc aatttcccgc tgccgcgctg
      961 aatgaggatc gaagacggac atagagactt cggcctttgg cgccggacac gtcgcccaca
     1021 atccagtagc tcccgcgtct tacgccattc ggcaaataat ggtggcatac ggcttcggcg
     1081 cacgcagcca gccgctgcgc cagatcggcg gcgatgctct ccatgatcgt tactcccatt
     1141 gaaagaggaa aacggccagc tcaatgggcg gtgggcggag agtgctcgaa gagccggctg
     1201 aaaatcgctg cacagccttc gccaagcgga acgaacaggc gcaagcgcca ggcgatgatt
     1261 tcgctgacaa ggccaagggc tttcaaggca gggaccgaac tctcgccaaa gcccgtgagc
     1321 tcgacgcggt cgcgccccat gaccttggct ttgcgcagcg tgagaccgtc gtgaagcctg
     1381 atcgggcgcg cctcgcttaa aagaaggtcg attgcaacct cgggcgaaag cgctccgtcc
     1441 ttggcgtcaa gcccgaaggc attccgaagt tcagcaagat catcgtgtct gatgaggcgg
     1501 ccgatcagcc gttcgccctc ggtggtggtg agccggaaga tgcggcagcc cttccggggg
     1561 agcttctgcc agaccggaag aagcagtccc gtcacgagat gcaggacatt gacccgcgtc
     1621 tcgggaaggc tcttgagttc ttctgcccac gccgccgcga aggcgccggc atcggcctca
     1681 ttccagaagg agtgttcgag atcggcaagc gccatgcttt cgcggctcaa gggccgttgg
     1741 aggcgcaatc tctcgatgac ttcgcctgct tcaccaagaa gcgagggggc agatgtggca
     1801 agcgccgcac gtccggtgcg ctcgttgacc agaagcctgt cgccatgctt tctggaaccg
     1861 aggatgctct ccagggcgat ggggttcagc ttctcatgct gctcgatggt gaggaggcag
     1921 gtctcagcgc cgctgtcgcc atgatgagcg agagtttcgc gaacgctgac gatgagcttt
     1981 tcgccagtca gcgtctcgag gccctggtcg tagaggccgg ccgcaatcgc accctcgata
     2041 cgcaccgtga gcagcgtctc gaactcctcg aacagcttgt tctggatgtc gatgggcagg
     2101 gcaagcaggc ggttcaggaa cgtcgtgatg ggcggaagct cttcgcgcaa agtgccgtcg
     2161 ccgtcgcaga gagaaagtcc ggtcgcctcc tcgaacgctt cgagcgtgag gcaggagcat
     2221 ttgccacgat agatgagatc atagagatgg cgaagcgcat cgcgggcata gacgctttcg
     2281 agattgtcgc cgggcctgaa caagccctgg cctccggtct ggcgctggcc gcgggtgatg
     2341 gcgccgaggg agtcgagccg cttcgcaatc gtcgagagga agcgcttctc cccgcgcaca
     2401 tccgtggcca caggccggaa aaggggcggc tgcgcctgat tggtgcggtt cgagcggcca
     2461 agcccctgaa tggcggcatc tgcccgccaa ccaggctcca gcaggtaatg gatgcgcttt
     2521 ctttggttct tcacgccgcg atcggcatgg tagctgcggc cggtgccccc cgcatcggag
     2581 aagacaagga tgcgcttgtc gtcatccatg aaggcctgcg tctcggagag attggccatg
     2641 gatggacggt tctcgacctg aaggatcggc tggccttgcg agtccagctt ccgcacaatg
     2701 cggcggctgc gtcccgttac ttcggccact tgatccgttc cgaagtgctg tacgatctga
     2761 tcgagcccac tcggcaccgc cggaaggctt gccagcttat cgatc
//
LOCUS       DATMGX010000762         2028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_81259, whole genome shotgun sequence.
ACCESSION   DATMGX010000762 DATMGX010000000
VERSION     DATMGX010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81259"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(624..1145)
                     /locus_tag="VNR65_12535"
     CDS             complement(624..1145)
                     /locus_tag="VNR65_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19542.1"
                     /translation="MTTKNETLKVVALEDGSSAVVKIDPANPRLLEVVATFYDAARAQ
                     RYAQMTEGQETEPAANVPEPKLPVAKVSEPKLHVTKVSEPKLPKASNGKSAEANGAML
                     DLSVRQNAVMNALRSKMDDNKQVEAKAASLAQAAQIPLGSLHSVLGSLEKKQLIINTR
                     PGTPRAPAVYQVL"
     gene            1519..>2028
                     /locus_tag="VNR65_12540"
     CDS             1519..>2028
                     /locus_tag="VNR65_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19543.1"
                     /translation="MGRNAIVGIVALGLLIASLGGAWHVLSENVEMRLTAGILSNYVR
                     NHPPKPWRGFEIEILNVSVDREVHIKAHVAGNLIHTPIEISGTPEYDANARAIFLHVS
                     RAGVPRDSARPMLSRFNSMLSPLATYIARNLAEIIPAKRIRAETPAGAMFLTTVRSVR
                     VDGDAVVVAL"
BASE COUNT          405 a          547 c          609 g          467 t
ORIGIN      
        1 atttcgataa gtatgtagaa agcgccgtga aaaggcccct aaacgatccc taacggttgc
       61 cccgtgatcc ccctcaccgc cccccgttag ggattttttc ttccccgccg ctgggcgtgt
      121 gtgcattggc gtgtgtgaga ggcctgcggg agaaggctcg aaaagtggat ttctcacggg
      181 caacgatcgc aataatccgt tagaaggctg gaacaagtca ggctttggct tgctcgcggg
      241 ggtcaggaca gctccacagg cggcgccttt ttgctcgggc gcggcttttt ttcgcgaaaa
      301 ctagcccctt cgtcgcgggg cgttcggctc ggcggcggtc tcatcaaggc gcctgaatcg
      361 cgattgccgg ccagcttcat gccctgcggc gcctaatgca agcgatcgcg ccttcgaaca
      421 cttaacagtc ggcgtcttcg agcttccaaa cagggccggt cacttttgcg atgaggtgtg
      481 ggtagcaatc ggggtgtgcg tgaccggcgc cgtcagaaga ttttgcaaag gtatcctgag
      541 ccggcgctac gaggacgtaa gccggatctt cctggcgctt gataggccct cagttgctcc
      601 tcgcaactga ggggaggggc tgttcagaga acttggtaga ccgcgggcgc gcgtggagtt
      661 ccggggcgcg tgttgatgat aagctgtttt ttctcaagcg atccaagaac cgagtgaagc
      721 gagcccagcg gtatttgcgc agcctgggcg agggaggcag ccttcgcctc cacctgctta
      781 ttatcgtcca ttttcgagcg aagcgcattc atcactgcgt tctggcggac gctgaggtcg
      841 agcatcgctc cgtttgcttc ggcgctcttt ccattcgagg ctttgggcag ttttggctcc
      901 gagacctttg tcacgtgcag tttcggctcc gagacctttg ccacgggcag tttcggctct
      961 gggacattcg ccgctggctc ggtttcctga ccctctgtca tttgcgcata gcgttgtgcc
     1021 cgtgcggcat catagaacgt cgccaccact tccagcaggc gagggtttgc gggatcgatc
     1081 tttacgacgg ctgaactgcc atcctcaaga gcaacgacct taagcgtttc atttttggta
     1141 gtcacagtta tttcctttcc cgcagacatc gcgcggcagt ccttatctcc gactgagcag
     1201 tccttggagc aaaaacagcg caatctgcat ggagtgataa cggattgtca gaacacagcg
     1261 ctacgcttgg tcttccaaac ccaactgggt agctaaacaa acttcgttga gtgggatttg
     1321 tcaatagatg acgagggaaa cactcacacc tgtgacacca gaggtggaaa acatgaggcg
     1381 actcaggccc gtgacgccta ggatccgcct gctggttgaa gaaggtctct ccattttcaa
     1441 ccctggcaag gtctggtcaa acgtcgcgca gttacattgc cgcaggcggc aggaccgtga
     1501 gccggagggg caaaacagat gggcaggaac gctatcgtag ggatagtcgc gcttggattg
     1561 ctgattgcga gccttggcgg cgcatggcac gtgctctcgg aaaatgtaga aatgcgtctg
     1621 acagccggta tactttctaa ctatgtgcgt aatcatccgc ccaagccctg gcgtggcttc
     1681 gagatcgaaa tcttgaatgt ttcggttgac cgcgaggtgc atatcaaagc ccatgtggcc
     1741 ggaaatttga ttcatacccc gattgagatc agcggtactc cggagtatga cgcgaatgcc
     1801 cgcgcgatat tcctccatgt gtcgagggcg ggagtgccgc gagattcggc gcgcccaatg
     1861 ctgagccggt tcaattcgat gctgagtccg ctcgcgacct atatcgccag gaacctggct
     1921 gaaatcattc cagccaagag gataagagca gaaacgccag ccggggcgat gtttctgaca
     1981 acggtgaggt cggtgcgtgt ggacggtgat gctgtggtcg ttgcgctc
//
LOCUS       DATMGX010000763         2962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_425402, whole genome shotgun sequence.
ACCESSION   DATMGX010000763 DATMGX010000000
VERSION     DATMGX010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425402"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..905
                     /locus_tag="VNR65_12545"
     CDS             177..905
                     /locus_tag="VNR65_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWJ19544.1"
                     /translation="MSLRSRHNLRLLGAAAVHVLTATGGVLGLLSLLSACDGQWTAAF
                     VWLGAALIVDGVDGPLARLVDVKRVLPRFSGEELDHLVDYITYVTVPAFMVARAEIVP
                     ESLRLPLAAVIMLVSLYHFSDTESKTADGYFVGFPALWNVVVLYCFVLGLAPAVSASI
                     IAICAVFTFIPLYWLHPLRVRRLRLVTFAVVSAWGAAAAGAILHGFPGTRVEQIIFAG
                     AAIYVIAIGVSAGAGSDRRFDRVK"
     gene            946..1812
                     /locus_tag="VNR65_12550"
     CDS             946..1812
                     /locus_tag="VNR65_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWJ19545.1"
                     /translation="MELLFDPAAWASLITLTALEVVLGVDNIVVLSILTHALPPKEAQ
                     RARAVGLGLALALRLALLSAISWITRLTYPVFSTGGQDFSWRDLILIGGGLFLMVKAT
                     QEIHAEIEGGEDPSGVPRKKASFGAVISQIAIMDLVFSLDSIVTAVGLARDIEVMVVA
                     ICISIGIMYAAAAPVGGFIKRHPTTKMLGLSFLILIGVALVADGFEIHIPRGYIYFAM
                     AFAGVVETINIWAKGSSSRRRPASSASGETFELEAEPQNPPALQAPLTKGGSMPHPAR
                     KMKAPRRRARKK"
     gene            1926..2837
                     /locus_tag="VNR65_12555"
     CDS             1926..2837
                     /locus_tag="VNR65_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ19546.1"
                     /translation="MRALIVSALVALSIAAATERCAAEQVTLDYLGLELSANLEIVSG
                     KSIKNEVVLLVHDTLGHDRMELMAALQDSLRDQSISSLAITLGLGMDKRHGMFDCAME
                     QDHRHEDAVDEIAGWVQWLKEKGATSITVAGQGRGANQVALYAINRLDKTVKRAVLMS
                     PLMQGEEKAEAEYSARFGQSLRQELAKAEELVASGRGNLFVDVPGFLTCSKPQVTAGT
                     FANYYSANAKFKTTNLLQSIKVPVLLAVGDADPVLSEVKAAEPEFSSLKTVTMVVIPG
                     ADQDFRDMGADDLARKIKEFIGRKLQG"
BASE COUNT          590 a          853 c          860 g          659 t
ORIGIN      
        1 ggtttgggac tctcgccact cttccgaaat tggatttcgg gcttgagctg ttgtacggcc
       61 cgaagagcgg atcggaagcg cttcagctcg atcagcatgc gcccgacaac gacatgcaga
      121 tcaagggcac gctgacccac aagttttaac tcggagccct gcgcgagttc ccttccatga
      181 gcttgcgctc aagacataat ctcaggcttc ttggcgctgc ggcggttcat gttttgaccg
      241 cgacgggcgg tgtgctcggt cttctctcgc ttctgtcggc ctgtgacggc cagtggacgg
      301 ccgccttcgt ctggctgggt gctgccctga tcgttgatgg tgttgacggc ccgcttgccc
      361 gcttggtcga tgtcaaacgc gttctacccc gtttctcggg agaagaactc gaccaccttg
      421 tcgactacat cacttacgtg acagtgccgg cgttcatggt tgcgcgcgcc gagatcgtgc
      481 cggagagcct gcggcttcct ttggcggcgg tgatcatgct cgtgtcgctt taccacttct
      541 ccgacaccga aagtaagacc gcggacggct acttcgttgg tttccccgcg ctctggaacg
      601 tcgttgtgct ctattgcttc gtgcttggtt tagcgccagc cgtttccgcg tccatcatcg
      661 caatctgtgc tgtcttcacg ttcataccgc tctattggct tcacccgctg cgggtgaggc
      721 gtctgcggct tgtcacattc gccgttgttt cagcctgggg cgcggccgct gccggcgcaa
      781 tccttcacgg ttttcctgga acgcgagtcg aacaaattat attcgctggt gcggcgatct
      841 atgtcattgc aatcggtgta agcgccggag ctggaagcga ccgccgtttc gatcgcgtga
      901 agtgagaaaa ctcccgaaca gaaggccgca tcggcggcag cttagatgga acttctcttc
      961 gatccggccg cttgggccag cctgatcaca ctgacggctt tggaggtcgt gctcggggtc
     1021 gacaatattg tcgtcctgtc gatcctcacg catgccctcc ctccaaaaga agcgcagcgt
     1081 gcaagggcgg tggggcttgg tctagcactt gccttgcggc ttgccttgtt gtcggcaatc
     1141 agctggatca cccgcctcac ctaccccgtc ttcagcaccg gtggacagga tttctcctgg
     1201 cgcgacctta tcttgatcgg tggcggcctc ttcctgatgg tgaaggctac tcaggaaatt
     1261 catgccgaaa tagaaggtgg agaagacccc tccggcgttc cccggaaaaa ggccagcttc
     1321 ggcgcggtca tatcccagat cgcgatcatg gacctcgtct tctcgctcga ttcgatcgtc
     1381 acggcggtcg gcctcgcgcg cgacatcgaa gtcatggttg tcgcgatctg catctcgatc
     1441 ggcatcatgt acgctgcggc tgcacccgtt ggagggttca tcaaacgcca cccgaccaca
     1501 aaaatgctcg gcttgtcctt tttgatcctg attggcgtcg cacttgtcgc agacggcttc
     1561 gagattcaca ttccgcgggg atatatctat ttcgcgatgg ccttcgcggg tgtcgtcgag
     1621 accatcaata tctgggcgaa aggctcctca agccgaagaa ggccagcatc ctccgcaagc
     1681 ggggaaacct tcgagcttga ggctgagcca caaaacccgc cggcgcttca agcaccgctg
     1741 accaaagggg gctcgatgcc ccatcccgca cggaaaatga aggcgccgcg gagaagagct
     1801 cgaaaaaaat aggccttgcc gccattttat caccatcagt gaggccatct tggcgccttt
     1861 ggcccagatt tcgtccataa gcgcgattag cgagagcact ggaaaagcct caaccggaaa
     1921 gaaccatgcg cgccttgatt gtctccgcct tggtggcgct ttcgattgct gctgcaaccg
     1981 agagatgcgc cgccgagcag gttaccctgg attatctcgg tcttgaactc tcggccaatc
     2041 ttgagattgt ctcgggcaag tcaatcaaga acgaagtcgt tcttttggtg cacgacacgc
     2101 tcggccatga ccgaatggag ctgatggcag cgcttcagga tagtctgcgc gatcagagca
     2161 tcagttcgct cgccataacg ctcgggctcg gcatggataa acgccacggc atgttcgatt
     2221 gcgcgatgga acaggatcac cgccatgagg acgccgtgga cgaaatcgca ggctgggttc
     2281 aatggctgaa ggagaaaggc gcgacctcca tcacagtcgc gggccaaggg cgcggcgcca
     2341 atcaggttgc gctctatgcc atcaaccgct tggacaagac tgtgaagcgc gccgtgctca
     2401 tgtcgccttt gatgcaaggc gaagagaagg ctgaggcgga atatagcgca cgttttggac
     2461 aatcccttcg gcaagagcta gcgaaggccg aggaacttgt ggcaagcgga cgcggcaacc
     2521 tgttcgtcga cgtgccagga ttcctgacat gctcgaagcc gcaggtaacg gccgggactt
     2581 tcgcgaatta ttacagcgcg aacgccaagt tcaagacgac gaacttgttg caaagcatca
     2641 aggttcccgt gctgcttgcg gtcggcgatg cggacccggt cctgagcgag gtcaaggcgg
     2701 ctgaaccgga attctcaagc ctcaaaacag taacgatggt cgtgatcccc ggagcggatc
     2761 aggatttcag agatatggga gccgatgacc tcgcccggaa aatcaaagag ttcatcggcc
     2821 gtaaattgca gggttagggg ttagcccgcg tccaaataga cgcggataag ctgctctgca
     2881 ctttagaacc gatcaggctt tccgcccttt taatcgattc cgattaaagg cgggtcgatc
     2941 taggatcgcc gcagtactac tt
//
LOCUS       DATMGX010000764         3152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_859,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000764 DATMGX010000000
VERSION     DATMGX010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..407)
                     /locus_tag="VNR65_12560"
     CDS             complement(27..407)
                     /locus_tag="VNR65_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19547.1"
                     /translation="MSIRKAASVAISICVFAALLFAGSDAGKSVSVNGYVLDSACAFT
                     KGLQKPISAQCAQACAKAGSPLVILADNGTIYWPIADTTPSAGQNDKLMPFAGQRVTA
                     SGKVYARGGSSAIVIEKIAAEAAK"
     gene            567..965
                     /locus_tag="VNR65_12565"
     CDS             567..965
                     /locus_tag="VNR65_12565"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HWJ19548.1"
                     /translation="MFLSMKQPPHKPTASELEILRVLWTRGPSTVREVHESLSEKKEL
                     GYTTVLKLLQIMTTKGTVKRNETQRAHVYEACLPAEQTKRQLAGDMLQRVFEGSASQL
                     MMHALAGSKSSPQEIEELRRLLDEYERSRK"
     gene            962..2923
                     /locus_tag="VNR65_12570"
     CDS             962..2923
                     /locus_tag="VNR65_12570"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HWJ19549.1"
                     /translation="MNTLVNWLSPSAMQATGWALLHFLWQGTALAALAAAAMALCRRA
                     STRYLLGVGALVLMLLVPLATFFVYSQQHSGVADTAKSSPLAVAQLTARDRGAASGST
                     QPSSSIRSSFAPSLDTLPWLVEAWLLGVAFFSLRSAGGFLLLERERRRQSSVVSERVL
                     EICYTLQDQLGLNRAIAYCECKWLQAPAVIGWFRPIVFLPVTALTGLSEEQLQVVIAH
                     ELAHIQRFDPFVNVFQVFVETLLFYHPAVWWLNKRIRAEREHCCDDMAVALCGNAVEY
                     ARALTLMEEWRSAPVFAMAANRGPLTERIVRVLGLKTLGAGMRGIGLTGSILCLTAAL
                     IAGNALRGIAHPPSVAAIWQAAAQPASASSPATPNSSAVAKPSAAHPQSTEQTTASPT
                     ASSYIDEMKAAGLTDLTVDQLIALKTQDVTPEYVKELRDQGLHPDANTLIAMRVQDIS
                     PEYVKGLRDAGLNPDQNQLIGLKVQGADGAYYRAMKEAGLQPDVNTLIGLKVQDVTPE
                     YVREIRAAGLAVNANQVIGMKVQDVTPAYLKAMHDLGIELRTDDVIGMKVQDVTPEYV
                     RGIQALGLKPSAGELIGLRVQDVTPEYIKALQSAGFKLDISDIIGAKVQDVTPEFIER
                     AKAHGFKDLTLQKLIQLRQLGILDSKADL"
BASE COUNT          641 a          925 c          937 g          649 t
ORIGIN      
        1 gagccgcgat gtgcgctggg atcgccctac tttgctgctt ccgccgcgat cttttcgatc
       61 acgatcgcgg atgatcctcc acgcgcgtat accttcccac tggccgtgac tctctgtccg
      121 gcgaaaggca tcagcttgtc attttggccc gcggagggcg tggtatcggc gatcggccag
      181 tagatggttc cgttgtctgc caggatcacc agcggcgatc cggctttcgc gcaagcctgc
      241 gcgcactgcg cgctgatggg tttctgcagg cccttggtaa acgcgcaagc tgaatcgaga
      301 acataaccgt ttaccgacac ggatttgccg gcatcgctgc cggcaaacag cagggccgca
      361 aacacgcaga tggaaatggc tacgctggct gcttttcgaa tgctcatgtt gaaccccttt
      421 tccttcacga ttcctgaatt caggtcaggt tccccagatc ggagcataag gtagcagact
      481 agggtggagg cgcaatagta aaaaccgact atacgttttc ctgtgtctac gaagattttc
      541 gttgacacta cgaatatctt cgtagtatgt tcctgtccat gaagcagcct ccgcacaaac
      601 cgaccgcttc ggaactcgaa attctgcgcg tactttggac tcgcgggcct tcgacggtac
      661 gcgaagtgca tgaatctctc agcgagaaaa aagagctggg ctacaccacg gtgctgaagc
      721 ttctgcaaat aatgacgaca aaaggcaccg tgaagcgaaa cgaaacccag cgcgcgcacg
      781 tctatgaagc atgcctccct gccgagcaga ccaagcgcca gcttgccggg gacatgttgc
      841 agagggtctt cgagggttcg gcgagccagc tcatgatgca cgccctcgca ggcagcaagt
      901 cttcgccgca agagattgaa gagttgcgcc gtctgctgga cgaatacgaa aggagtcgca
      961 agtgaacact ctcgtgaatt ggctctcgcc gagcgcaatg caggctacag gttgggcgct
     1021 cttgcatttc ttatggcagg gaactgctct cgctgccctg gcggcggctg cgatggctct
     1081 gtgccgtcgc gcgtcgacgc gctacctgct gggagtgggc gcgctcgtgc tgatgttgct
     1141 ggttccgctg gcgacgttct tcgtctattc gcagcagcat tccggtgtgg cagacaccgc
     1201 gaagtcgtcg ccgctcgccg tggcccaact aactgcgagg gacagaggcg cggcgagtgg
     1261 ctcgacccaa ccgtcttctt ccatacgatc gtctttcgcc ccttcgctcg acacgctccc
     1321 atggctggtt gaagcctggc ttctcggcgt cgcgttcttc agcctgcgtt ctgccggtgg
     1381 attcctgctg ctcgagcgcg aacgccgcag acagtcgagc gtggtcagcg agcgagtgct
     1441 ggagatttgc tataccctgc aggaccaact ggggttgaat cgagccatcg cctactgcga
     1501 gtgcaagtgg ctgcaggcgc cggctgtgat cggatggttt cgtcccattg tgttcttgcc
     1561 cgtcacagct ctaactggcc tttcagaaga gcagcttcag gtggtgatcg cccacgaact
     1621 ggcgcacatc cagcgcttcg atccttttgt gaacgtgttc caggtgttcg tcgagacgct
     1681 gctcttctac catccggcgg tgtggtggtt gaacaagcga attcgggcgg aacgcgagca
     1741 ctgctgcgat gacatggcag tggcgctctg cggtaacgcc gtcgagtatg cccgcgctct
     1801 tacgctgatg gaagaatggc gcagcgcgcc ggtctttgcc atggccgcga accgaggccc
     1861 gctgaccgag cgcatcgtcc gcgtcctcgg actgaagacc ctgggcgcag gaatgcgcgg
     1921 catcggacta accggcagca tcctctgcct gactgccgcg cttattgctg gaaatgcttt
     1981 gcgtggaatc gcgcatccgc cgtcggtagc cgcgatctgg caagcagcgg cccagcctgc
     2041 gagtgcttcc tctccagcca ctcctaacag ctccgctgtc gcaaagcctt cggcggctca
     2101 tccgcaaagc accgagcaga caacagccag tccaacggcg tcgtcttaca tcgacgaaat
     2161 gaaggccgca gggctcaccg atctgaccgt cgaccaattg atcgcgctga agacgcaaga
     2221 tgttactccc gagtacgtca aggaacttcg ggatcaaggc ttgcaccccg acgcgaacac
     2281 cttgattgcg atgcgggtgc aggacatctc tccagaatat gtgaagggac tgcgcgacgc
     2341 gggcctcaat cccgatcaga accaactcat cggtctcaag gtccagggcg cggacggcgc
     2401 ctattaccgc gctatgaagg aagccggtct gcagcctgac gtgaacacgc tcatcgggct
     2461 gaaggtgcag gacgtcactc ccgaatatgt tcgcgagatt cgcgccgctg gcctcgcggt
     2521 aaacgctaat caagtgatcg gcatgaaggt gcaggatgtt acgcccgcgt acctgaaagc
     2581 tatgcacgat ctggggattg agcttcggac cgatgatgtg atcggcatga aggtgcagga
     2641 cgtaacaccg gagtacgtgc gcggcatcca ggctctgggc ctcaaaccgt cggccggcga
     2701 gttgattggc ctgcgggtgc aggatgtaac gcccgaatac atcaaggcgc tgcagtcggc
     2761 aggattcaag ctcgacatca gcgacatcat cggggccaag gtgcaggatg taacgccaga
     2821 gttcatcgag cgcgccaagg cgcacggctt caaagatctg actctgcaga aactgataca
     2881 gcttcgacag ctgggaattc tggattcgaa agctgacctc taaacgcagt tgtggtgagt
     2941 gcgcggggct tcgcccacgc tggacggccg aggcggccat cgccacataa acaattaacc
     3001 cacttcatct gaggagtctt catgagaatg cgccgaactc tcgcgtacct ggttttcgca
     3061 tcgtgtgtga tcgtattcgt ggtcaagaat cgcgccgccg aagtgcacag cggcgactgg
     3121 accatcagca agtcagacat tcccggcaag gt
//
LOCUS       DATMGX010000765         2376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_39661, whole genome shotgun sequence.
ACCESSION   DATMGX010000765 DATMGX010000000
VERSION     DATMGX010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39661"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VNR65_12575"
     CDS             <1..367
                     /locus_tag="VNR65_12575"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWJ19550.1"
                     /translation="TGLLLGTVSLGSAQEPASQQAAPAADNTKMNQGDQNKRVPTADQ
                     QKENRSDRDITQQIRQSIMNDKSLSTYAHNVKIITQNGQVTLKGPVRSESEKQTIETK
                     ATEVAGANKVTNDLNVKSQ"
     gene            398..955
                     /locus_tag="VNR65_12580"
     CDS             398..955
                     /locus_tag="VNR65_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HWJ19551.1"
                     /translation="MAGKNTAVFGIYPNRASVDNAVDALKSADFRNTDISVLFPENSG
                     TKDFAHEKNTKAPEGTATGAGTGALLGGGLGWLVGIGALAIPGLGPFIAAGPIMAALA
                     GAGVGGAVGGLTGALIGMGIPEYEAKRYEGRVKDGGILLSVHSDDSAWTKKAKEILER
                     TGAQDVSSTGEASAEKDANTPVAVA"
     gene            1154..1741
                     /locus_tag="VNR65_12585"
     CDS             1154..1741
                     /locus_tag="VNR65_12585"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWJ19552.1"
                     /translation="MALKEVLIDELRDMYSAENQLVKALPKLAKGAKSPKLQELFAAH
                     LEETKGQVERLKEVFEKLEEKPTGSHCNGMEGVIEEGADALEKDEEGASFDSGLIGAA
                     LRTEHYEIAGYQASIAMAKALGMTEIASLLTDNLDEEVAAAEKITAAARPILAQSAGE
                     PEAHKKPLSAKEKYSAEKSREDEKKAAPDLKKRAS"
     gene            1935..>2376
                     /locus_tag="VNR65_12590"
     CDS             1935..>2376
                     /locus_tag="VNR65_12590"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWJ19553.1"
                     /translation="MSIFDQHTARVLATIAALMAVAAFLYGVRHTLVAMLFAVLFAYL
                     LEPIVFRIEKSMFARGSRWLAILETYLVIASILTALGILFGPTLVEDTRLLSQSLPGL
                     LEKVTTGKIVWQFGNRYGWSYDTQIKIEQLIAAHQTEILAWSGRA"
BASE COUNT          592 a          665 c          692 g          427 t
ORIGIN      
        1 caccgggctc ttgctcggga ccgtgtcctt gggaagcgcg caggaacccg ccagccagca
       61 ggctgctccc gctgcggaca atacaaagat gaaccagggc gatcagaaca agcgcgtgcc
      121 cacggcggac cagcaaaaag aaaatcgttc cgaccgcgac atcacccagc agattcgtca
      181 atccatcatg aatgacaaat cgctgtcgac ctatgcccac aacgtcaaga tcatcacgca
      241 aaacggtcag gtcacgctga aagggccagt gcgctccgag agcgagaaac aaaccattga
      301 gaccaaggct accgaagtcg cgggcgcaaa caaggtgacc aacgacttga acgtcaaatc
      361 acagtaatac cacccggcac caaaaaggag aaacaccatg gcaggcaaaa acacagcagt
      421 atttggtatc tacccgaacc gcgcaagcgt ggacaatgca gtcgatgcat tgaagtcagc
      481 agactttcgg aataccgaca tttcagtgct attccccgag aattccggca cgaaggactt
      541 cgctcacgaa aagaacacca aggcccctga aggcacggca actggcgccg gaaccggtgc
      601 tttactgggc ggcgggttgg gctggttggt aggcatcggc gcactcgcaa tacccggcct
      661 ggggccgttc attgctgccg gcccgatcat ggcggctctc gcgggagccg gcgtcggcgg
      721 cgcagtcggt ggcctcacgg gcgctctcat cggcatggga atcccggaat atgaagccaa
      781 gcggtacgaa ggccgcgtca aagatggggg gattctcctg tcggttcact ctgacgattc
      841 cgcgtggaca aagaaagcca aggaaatctt ggagcggact ggtgcccagg atgtctcctc
      901 gaccggcgaa gcaagtgccg aaaaggacgc gaacaccccc gttgcagttg cctaacctgg
      961 ggtagcgcta cttagtcgcg gagttgggcg gcggcggaat agtctgccgc cgcccttcta
     1021 taaatctttt cttgaccggg caggtctttg ccgacggcgt gccctgaagg cgcgcgatgg
     1081 gaccaagtta cagcctctcg attgacgctg aaagtttaat aacccagaaa aacagactta
     1141 ggagacagct aacatggcac taaaagaagt tttgatcgac gaactacgtg atatgtacag
     1201 cgcagaaaac cagctagtaa aggctctgcc gaaattggcg aaaggcgcca agagccccaa
     1261 gctgcaggag ctttttgcgg ctcacctgga agagacaaaa gggcaagtgg agcggctgaa
     1321 ggaagtgttt gagaagctcg aagaaaagcc caccgggtcg cactgcaatg gcatggaagg
     1381 cgtcatcgag gaaggcgcgg acgctttgga gaaggacgaa gagggcgcgt cctttgactc
     1441 aggtctgata ggcgctgccc tccgcacaga gcattatgaa atcgccggct accaggcgag
     1501 cattgcgatg gcgaaggctc tcggcatgac tgagattgca tcgctgctga ccgacaattt
     1561 ggacgaagag gtcgctgcag ccgaaaagat caccgccgcc gcccggccca tcttggccca
     1621 aagcgccggg gagccggaag cacacaagaa gcccctgtcc gctaaggaga agtactccgc
     1681 ggaaaagagc cgcgaggacg aaaagaaggc agctccggat ctcaagaaac gcgcatccta
     1741 gagaaatcag ttctggcagc cccgcggcgg cggggctttc ctcttggaca agaaaccgcc
     1801 cagccgaaaa aagaccgcga tgtgccacaa ctttccccct tttcacgcca tcgatttcag
     1861 ttggcaggtt ggcctcgcgt tgttcgagcg gtctataaga actctggctg acatctaagg
     1921 accgtaaaca gtcaatgtcc atattcgatc aacatacagc aagggtactg gcaacgattg
     1981 ccgctcttat ggctgtcgcg gcctttcttt atggtgtgcg ccacacactc gtcgccatgc
     2041 tgttcgctgt cctcttcgct tacctgctgg aacccatcgt cttccgaatc gaaaagtcga
     2101 tgtttgccag gggctcgcga tggcttgcaa tcttggagac ctacctggtg attgcgtcga
     2161 ttttgacggc gctcggcatt ctgttcggac cgacgctggt ggaagacacc cgcctgctaa
     2221 gccaatcgct gcccgggttg ttggagaagg ttacgaccgg aaagatcgtc tggcaattcg
     2281 gcaaccggta cggctggagc tacgacactc agattaagat cgaacagctg attgcggccc
     2341 atcagaccga gattctcgcc tggagcggac gggcag
//
LOCUS       DATMGX010000766         9202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_180610, whole genome shotgun sequence.
ACCESSION   DATMGX010000766 DATMGX010000000
VERSION     DATMGX010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9202
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180610"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VNR65_12595"
     CDS             <1..968
                     /locus_tag="VNR65_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ19554.1"
                     /translation="QVFRFAAPSLMAGNTAVLKHASNVPGCALAIEDVFKEVGFPANV
                     FSTVLIPSRDAEALIANPAIAAVTLTGSVAAGQSVAAAAGRNLKKSVLELGGSDAYLI
                     LEDADIERAASVSTAARMVNGGQSCIAGKRFIVVSAVKEAFEKAFTAKMHAIQMGDPS
                     DPETKLGPLVSVEARDAVHRQVQESIAKGAFLLCGGKIPEKPGAWYPPTVLSDVKPGQ
                     PAHDAEVFGPVAAIIEARDEEDAIRIANDSQFGLGSGVITSDIARGERIAAEMLEAGM
                     SFVNDNVRSDPRLPFGGVKHSGYGRECSHFGIREFVNIKSVLVRD"
     gene            1196..2536
                     /locus_tag="VNR65_12600"
     CDS             1196..2536
                     /locus_tag="VNR65_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057144789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HWJ19555.1"
                     /translation="MRQERTTQVSLFDGFAGHETGRELKAMSEWLDGHRELLDLVTKD
                     LCRRRVTNTGRHGLPAESALRCGLLKQHRQLSYEELAFHLEDSASFRSFARLPVAWTP
                     KKSVLQKTIGAIRAETWEAINQTLLGGARRQKVEDGSVVRQDSTVTAALMHEPSDSSL
                     LWDAVRVMVRLLKEADGLFGGGALVWRDRRRAAKKRARAIQFIRGQPKRVQLYRELIG
                     IARAARADLRQTQARLAKQEDPQSVRWLAKASHYQPLIERIIAQSERRVLKGQQVPAG
                     EKLISLFEPHADIIVKGGRDASYGHKLNLVTGRSGLVLDIVIEAGNPADSDRLLPMLE
                     RHIAFYGQAPRQAAADGGYASHANLDKAKARGVSDMAFHKKCGLRIEDMVKSRWVYRK
                     LRNFRAGIEAGISCLKRAYGLARCTWRGLEHFKAYVWSSVVAYNLALFARLTAA"
     gene            2955..4514
                     /locus_tag="VNR65_12605"
     CDS             2955..4514
                     /locus_tag="VNR65_12605"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: protein motif:HMM:NF010584.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HWJ19556.1"
                     /translation="MKAYLIGGGIGSLAAAAFMVRDGGVPGSAITIYEKLNILGGSLD
                     GARLPDGAYVVRGGRMLTFGHYECTWDLFDMIPSLEHPGKTVRQDSEAFNEEFKSHSR
                     ARIVDRNRHKVDVSKMGFTMSDRAELLALTEASEEELGADRITDWLSPAFFENNFWHL
                     WQTTFAFQPWHSAVELKRYLHRFMNEFPRIDTLAGVRRTVYNQYDAMVRPLSVWLGKQ
                     GVQFISGAEVVDIGVVEEGGLVRVNELKVQKGADLTTVAVAEDDLVFLQNASMTDASS
                     LGSMTTPPQQLTKTDSQGWALWEKLAKGRPEFGNPAAFNSSIPEAWWESFTVTCPTTT
                     FFDQMEAFSGNVAGTGGLVTFKDSNWLMSVVLCHQPHFTGQPDNLQVFWGYALHPDRI
                     GNFVAKPMADCTGAEILIEVCGHLNFDRNVFDGAICIPCRMPYITSMFMPRRRSDRPL
                     PVPKNSKNLAFVSQFVEIPEDVVFTVEYSVRAAQMAVYQLLGIKREVPPVTRYDHSFR
                     TILDSVAKALS"
     gene            complement(4718..5218)
                     /locus_tag="VNR65_12610"
     CDS             complement(4718..5218)
                     /locus_tag="VNR65_12610"
                     /inference="COORDINATES: protein motif:HMM:NF017277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HWJ19557.1"
                     /translation="MDSNETNLTSQTADIVSAYVASNALSSDKLADFISSVYGALSRA
                     SVHSAEPQKVDLKPAISIKKSVTPEFIICLEDGKKFKSLKRHLRSQYDMSPEEYREKW
                     GLPHDYPMVAPAYAAARSDLAKNMGLGRRGTAVAGTQPEVVAKVDPEKPGKPQRKQTR
                     KAGSRK"
     gene            complement(5736..6056)
                     /locus_tag="VNR65_12615"
     CDS             complement(5736..6056)
                     /locus_tag="VNR65_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ19558.1"
                     /translation="MIAMELQESLEKAGATVVGPVGRLDRALSKAKNDGLNAALLDVD
                     LNGERCWPVADLLSRAGVPFAFTTGFSADIVMPERFAGRPVLAKPYREGEILSAIRKL
                     LSLP"
     gene            complement(6136..8376)
                     /locus_tag="VNR65_12620"
     CDS             complement(6136..8376)
                     /locus_tag="VNR65_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HWJ19559.1"
                     /translation="MIIVIARAEKEYTLQHATVESAEQLWWGMKELAAARRISNLTDC
                     DREPIHIPGSIQPHGVLLAVDPASLKVVQIAGDTKRLLGLDTKDLLGHGVEAFVTQAE
                     VESLKALAAQEVTIPRSVFAFEMKVQHRGETLDATVHQSGGALVVELEPCLARPPINP
                     LLLVQGMVTRVHKAPDMAGFLQAITEEVRSATGFDRVMVYQFLPDNSGAVIAEAKHQD
                     LEPFLGLRYPASDIPRQARDLYLLNRMRIIPDATYTPAALIPPINPITGNPLDLSFSV
                     LRSVSPLHLEYLANMGVDASMSLSLVIEDRLWGLIACHHRMPRYVPHAVRSACELFAQ
                     MISLQLGEKLLSEVQAERFRMKEVHAGLVEAMVGQDDLGERLVDARPNLLDYIPAEGV
                     AVWWEGKVTTRGTTPTDQQLGSLVEWLNTSVPEGVFLTDCLASHFAPARNYTGVASGL
                     LALSVSRTPRDYVLWFRPEVSKTVTWAGNPAKPIDVTGDGLRRSPRKSFAAWKEIVRE
                     HSQPWGEPVADAAQALRLSILDVVLRHQDKVMREREEARRHQDFLMAELDHRVKNTLA
                     TIQALVKYTSSGAESLEEFAQKVQERVHAMARAHSHLTHSRWEGVNLKTVVAEQFAPF
                     GERVSAAGPDLALRPKAALSISLAFHELMTNAAKYGALSVPSGGVTISWEVQSRDGGR
                     GLLFRWEEHGGPPVAPPVRMGFGRMLLERSLAYDIDGEVTLDFRPEGLACEAFIPFAH
                     VIGLEE"
     gene            8791..9084
                     /locus_tag="VNR65_12625"
     CDS             8791..9084
                     /locus_tag="VNR65_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19560.1"
                     /translation="MVGAFIMAIGVALSAVFCWRQIMGQPAQAVQKANGSIALPVLKL
                     LLIVAALVAIGFSMIVIDLPNKNYLWIGAEVVVAVLFIMSGVSYLKQIFGGKR"
BASE COUNT         1956 a         2586 c         2673 g         1987 t
ORIGIN      
        1 ggcaggtgtt ccgcttcgcc gcgccttcgc tgatggcggg caacacggcc gtcctcaagc
       61 acgccagcaa cgtgcccggc tgcgcgcttg ccatcgagga tgtgttcaaa gaggttggtt
      121 ttccagcgaa tgtcttcagc accgtgctca tccccagtcg agacgcagag gctctcatcg
      181 caaatccagc cattgcagcg gtgacgttga cgggcagcgt cgcggcgggg cagagcgtag
      241 cggcggcagc gggccgcaac ctgaaaaaga gcgtgctcga actcggcgga agcgacgcct
      301 acctcattct cgaggatgcc gacatcgagc gcgccgctag cgtctcaact gccgctcgca
      361 tggtgaacgg cgggcagagc tgcatcgcgg gcaagcgctt cattgttgtc tcagctgtca
      421 aggaggcctt cgagaaggcc ttcacggcca agatgcatgc catccagatg ggcgatccca
      481 gcgaccccga gaccaagctt ggcccgctcg tgagcgtcga ggcccgcgat gccgttcacc
      541 gccaagttca ggagagcatc gccaaaggcg cgttccttct ctgcggcggg aaaatccctg
      601 agaagccggg cgcgtggtat ccaccgaccg tgctcagcga cgtgaagccg ggtcagcctg
      661 cccatgacgc ggaggttttc ggcccggtcg cggccatcat tgaagcgcgt gacgaggaag
      721 acgccattcg catcgccaac gactcgcagt tcggtctcgg ttccggcgtg atcacgtcgg
      781 acatcgcgcg gggcgagcgc atagcggccg agatgctcga agctggcatg agcttcgtga
      841 acgacaatgt gcgctccgat ccgcggctgc cctttggcgg cgtaaagcat tccggctatg
      901 gccgcgaatg ctcgcatttc ggcatccgcg agttcgtgaa catcaagagt gtgctcgtcc
      961 gcgactagag ttagcccgcg ccatctgtac gtggcttagc tgctcccatt catctgctct
     1021 gggtcgagag caaagatgac cttaggactc tcttgtgtcc atccgtgaag gcataagcca
     1081 ttgaaaagac tgaggcaatt tggcatttcc ctgatgcgca tccggctgga ttcgagtact
     1141 ctctaatagc aagtcattga ttctaaagac aagcttttgc cgggggaact cacaaatgcg
     1201 ccaagaacgc accacgcaag tgagcctgtt cgatggtttc gccggtcacg agaccggccg
     1261 ggagttgaag gctatgtctg agtggctgga tggtcatcgt gaactgctgg acttggtgac
     1321 gaaagatctg tgccggcgcc gggtgacaaa caccggacgg catggcttgc cggcggaatc
     1381 ggcgctgcgt tgcgggctac tcaagcagca tcgtcagttg agttacgagg aactcgcgtt
     1441 ccatctggaa gattcggcgt cgtttcggtc gtttgcgcgc ctgcccgtgg cgtggacccc
     1501 gaaaaagtcc gtgctgcaaa aaaccatcgg cgctatccgg gccgaaacgt gggaggcgat
     1561 caaccagacg ctgcttggtg gcgcaaggcg acaaaaggtg gaagacggca gcgttgtgcg
     1621 gcaggacagc acggttacgg cggcgctcat gcacgagcca agcgacagca gcctgctctg
     1681 ggacgccgtg cgggtgatgg tccggctgct gaaggaagcc gatggcttgt ttggcggcgg
     1741 cgcccttgtc tggcgcgatc gccgccgcgc ggcaaagaaa cgggcgcgag ccattcagtt
     1801 tattcgcggc cagcccaaac gggttcagct ctatcgtgag ttgatcggaa tcgcgcgcgc
     1861 ggcaagagcc gatctgcgcc agacgcaggc gcggctggca aagcaggagg atccacaaag
     1921 cgtgcgttgg ctggccaagg ccagccatta ccagccgctc attgagcgga tcattgcgca
     1981 gagcgagcgg cgggtgctga agggccaaca ggttccggcc ggcgagaaac tcatcagcct
     2041 gttcgagccg cacgccgata ttatcgtcaa aggcggtcgc gatgccagtt acggccataa
     2101 gctcaacctt gttacaggca ggagcggctt ggtcctcgac attgtgatcg aagcgggcaa
     2161 tccggccgat agcgaccggc tgctgccgat gctggagcgc cacatcgcct tttacggaca
     2221 ggcgccgcgg caggcagccg ccgatggcgg ctatgcgagc cacgccaatc tcgacaaggc
     2281 gaaggcccgt ggcgtcagcg acatggcctt ccataagaag tgcgggcttc gcatcgaaga
     2341 tatggtgaaa agccgttggg tctaccgcaa gcttcgtaat ttccgcgccg gcatcgaggc
     2401 gggcatatct tgcctgaaac gcgcttacgg gcttgcccgc tgcacgtggc gcgggcttga
     2461 gcacttcaag gcttacgtct ggtcctcggt agtggcctat aaccttgccc tgttcgctcg
     2521 cctaacagcc gcttgaggcg ccagcgatcg cgcagcccag gttaagcggc agtagccgac
     2581 gtttcctatt gtcaggaagc gccggcgggc ggtcatctga atgatttgca cccgatccgc
     2641 cgcctccgat ccatgaacaa gcacgcgtcg cgaaacaata agcgcttgat ctaacaacgg
     2701 aaacagccgt ttatggacgc gaactagcta ggcggcgacc gccgtggacg gcatgcagat
     2761 cacgctgcag gtagcgctgt cgaagtatgc tctacacgat acaagcaaac ccaaggggcg
     2821 ttgccgaata tcgatcgacc caagcacccg cagcgccgtt gactccaagc tcggatgacc
     2881 cgatgcggcc ataataaaga gttagagcgc aatgggatat ttagctcttt agataagcct
     2941 gaatgaggtt tctaatgaaa gcgtatctta tcggcggggg catcggttcg ttggccgctg
     3001 ctgccttcat ggtccgggac gggggcgttc ctgggagtgc catcacgata tatgaaaagt
     3061 tgaacattct cggcggcagt ctcgacggag cgcgcctgcc cgacggtgct tatgtcgtgc
     3121 gaggcggccg catgctgacc ttcggccatt atgaatgcac ttgggatctg ttcgacatga
     3181 ttccgtccct tgagcatcct ggcaagacgg tgcggcaaga ctcagaagca ttcaacgaag
     3241 aattcaagtc gcattcgcgg gcgcgtattg tcgaccggaa cagacataag gtcgatgtct
     3301 cgaagatggg tttcaccatg tccgaccgcg cagaacttct tgcgctgacg gaggcttccg
     3361 aagaggaact cggcgcggat cgcatcaccg attggctttc gcctgcattt tttgagaata
     3421 atttttggca tttatggcag acgactttcg cttttcagcc ctggcacagc gctgtcgaac
     3481 tcaagcggta tctgcaccgt ttcatgaatg aattcccgcg tatcgacacg ctcgctggcg
     3541 tgaggcgcac ggtttacaat cagtatgacg cgatggttcg ccccctgtct gtgtggcttg
     3601 ggaaacaggg cgtgcagttt atttccggcg cggaagtcgt tgacattggc gtcgttgaag
     3661 aaggtggcct cgttcgcgtg aacgaactca aagtgcaaaa aggtgccgat cttacaactg
     3721 ttgctgtcgc agaagacgat ctggtctttc tgcagaatgc gtcgatgacc gacgcgtcaa
     3781 gcctgggctc gatgacaact ccgccgcagc agttgaccaa gaccgatagc cagggctggg
     3841 cgctttggga gaagctcgcg aaggggcggc cggaatttgg caaccccgca gccttcaatt
     3901 caagcatccc tgaggcctgg tgggaatcct tcaccgtgac ctgcccgacc acaaccttct
     3961 tcgaccagat ggaggcattc agcggcaacg tcgcgggaac aggcggtctt gtgacattca
     4021 aggattcgaa ctggctgatg tccgtggtgc tctgtcacca gccgcatttt accggtcaac
     4081 cggacaatct gcaggttttt tggggatacg ccttgcatcc ggaccgtatc ggcaatttcg
     4141 tcgccaagcc aatggcagac tgcacgggcg cggaaatcct cattgaggtc tgcggccatc
     4201 tcaatttcga ccgcaacgtc tttgacggcg cgatctgcat tccctgccgc atgccctata
     4261 tcacatccat gttcatgccg cgcagaaggt cggaccggcc cctgccagtg ccgaaaaact
     4321 ccaagaacct cgcctttgtc agtcagttcg tcgagatccc ggaagacgtc gtgttcacgg
     4381 ttgaatattc tgtgagggca gcgcagatgg cggtctacca gctcttaggg atcaagcgcg
     4441 aggtgcctcc tgtcacaaga tatgatcact cttttcgaac cattctcgac tctgtcgcca
     4501 aggctctttc ttgagcgcca tcggagcgat tgatcggcct gaatctcacg ggtgagcaaa
     4561 agttcacacc agacccggcg cgctcatttc cgccgccgga caggccaccc tgctctgatc
     4621 aggcgctata gcgcgcggcc acaaagtcga ccgtaaagtg ctagagcagc tattcgcgtc
     4681 caaatggacg caggctgctc tagcgaccaa tgcgcaacta cttccgcgag ccagcttttc
     4741 gagtttgctt gcgttgcggc tttcctggtt tctcggggtc caccttagcg accacttcgg
     4801 gttgagtgcc cgctactgcg gtgccgcgcc ggcccagacc catgttcttg gccagatcag
     4861 aacgcgcggc ggcataggcc ggtgcgacca tcggataatc atgcggcagt ccccactttt
     4921 cccggtattc ttcgggggac atgtcgtact ggctccgcaa atgccgcttg agagatttga
     4981 atttcttgcc gtcttcaagg caaatgatga actccggcgt caccgatttc ttgatggata
     5041 tggccggttt caaatcgact ttctgcggct cggcagaatg gacggaggct ctgctcaaag
     5101 cgccatagac cgagctgatg aagtcggcaa gcttgtcact gctaagcgca ttactggcta
     5161 cgtacgccga cacgatatcg gccgtttgcg acgtgagatt cgtttcattg ctgtccatat
     5221 tcttgctcca gacctaagtg agggcacaga accaagaaac ggataaatgc gcccaatctt
     5281 gtcgataacg tttaccttgt gcttcgaacc tgtatttcga gaaggcaacg ccgttctatc
     5341 attattattt tcgcttacga aagagtaaaa ggcctttgcg cttctgattc taccaaatta
     5401 cgttcattca tgggcaatga cctgtattgg ttggttcttg caggccgatt gatgccggtt
     5461 gaccatgata atgttcaagg aagtaatatc tgcgaaggca agctcgccgt cgccaaaatc
     5521 aaagtggagg ttttcgtaac aaggccggcc cgtcttcgcg cagcggcgtt aggcctcggc
     5581 cggattgctt tcgtcttcgc ccgagatgcc ttcgaagtcg aggggccgtc ctaatctctc
     5641 ccccaaggcc tgccgacctc aatgaaacga acggtcccgt caggatgaag gagcggtcgg
     5701 gttgacaatt cgcttgccgg attagcgacg tatgctcagg gtaagctcaa cagcttgcgt
     5761 atggcggaca agatctcgcc ttcccggtag ggtttagcga ggacgggacg tcctgcgaac
     5821 cgttccggca ttacaatgtc tgccgaaaaa cccgttgtga aggcaaaagg aacgccggca
     5881 cgcgataaga gatcggccac aggccaacac cgttcaccgt tcagatcaac atccagcagg
     5941 gcggcattca gcccgtcatt tttggctttg cttaacgctc ggtcgagccg tccaacggga
     6001 ccaacgacgg ttgcgcctgc tttttcaagg ctttcctgca actccattgc gatcaaaaat
     6061 tgatcctcta cgaccaaaat cctctgtccg gcgagtgaca aagttggcgt actcgcttct
     6121 gccaagggcg acatcctatt cctcaagtcc gatgacatgt gcaaaaggaa tgaaggcctc
     6181 gcaggccaag ccttcagggc gaaaatccaa ggtaacttcg ccatcaatat cgtaagcgag
     6241 actgcgctct aacaacatgc ggccgaaccc catgcgcacc gggggcgcga ccggcgggcc
     6301 accgtgctct tcccaacgga aaaggagccc acgccctcca tcgcggcttt gcacctccca
     6361 gctgattgtg acgccccctg aagggacgga aagcgcgcca tactttgcgg catttgtcat
     6421 caattcatgg aatgcgagcg aaatgcttag cgcagccttg ggtctcagcg caaggtctgg
     6481 tcccgcggca cttacccgct ctccgaaggg cgcgaactgc tcagcgacca ctgtttttag
     6541 attgacgcct tcccaacggc tatgagtgag gtggctgtgg gcgcgcgcca tggcgtggac
     6601 gcgctcttga actttttgcg caaattcttc aaggctttcg gcaccgctcg aggtatattt
     6661 aacaagagct tggatggtgg ctagcgtgtt cttcacccta tggtcaagct ccgccatcag
     6721 aaaatcctga tgcctgcgcg cctcttcgcg ctcgcgcatg actttgtctt gatgccgtag
     6781 aacgacgtcc aggatcgata ggcgaagcgc ctgcgcggca tctgcgacag gctcgcccca
     6841 gggctgcgaa tgctcgcgca caatttcttt ccaggccgca aaggattttc ttgggctgcg
     6901 acgcaaacca tcgccggtaa cgtcgatggg cttagccggg ttgccggccc aggtcaccgt
     6961 tttcgatacc tccggacgga accagagcac gtagtcacgc ggcgtgcgcg agactgagag
     7021 cgcgagaagt ccgctcgcca cgccggtata gtttcttgct ggggcaaaat gtgacgcgag
     7081 gcaatcggtc aagaacacac cttctggcac agacgtgttc agccactcca ccagggaacc
     7141 caattgttga tccgtaggcg ttgttccacg tgttgtgact tttccctccc accaaacggc
     7201 aaccccttct gcagggatgt agtcaagcaa gttcgggcgt gcatcaacca atctttcgcc
     7261 aagatcatct tggccgacca tggcctcaac aaggcccgcg tggacctctt tcatgcgaaa
     7321 tcgttcggcc tgaacttcgc tcaagagttt ttcgccaagt tgcaaagaaa tcatctgagc
     7381 gaataactcg caggccgaac ggactgcatg cgggacatat cggggcatac gatgatgaca
     7441 agcgatcagc ccccaaagcc tgtcttcgat aacaagggag agcgacatcg aggcgtcaac
     7501 gcccatattg gccaaatatt cgaggtgcag aggggaaacg gagcggagca cactgaagga
     7561 gagatcgagc gggtttccgg tgatcggatt gatagggggt atcagggcag ccggcgtata
     7621 agtggcgtct ggaataatcc gcatccgatt gagcaagtaa aggtctctcg cttgtcttgg
     7681 aatatcggaa gccgggtagc gcagtccgag aaaaggttcg aggtcttgat gcttcgcctc
     7741 ggcgatgacg gcgccgctgt tgtccggcag aaactggtaa accatcacgc gatcgaagcc
     7801 ggttgctgag cggacctctt ctgtaattgc ctgtaaaaag ccggccatgt ctggggcttt
     7861 gtgaacgcgc gtgaccatgc cttgcacaag caagagcggg ttgatgggcg gcctcgccaa
     7921 acagggttcc agttccacca ccaaggcgcc gccgctttga tggacggttg cgtcgagcgt
     7981 ttctccacgg tgctgaactt tcatttcaaa ggcgaagacg ctgcggggga tcgtgacctc
     8041 ctgtgcggca agcgccttga ggctttcgac ttcggcctga gtgacaaacg cttccacccc
     8101 gtggccaaga aggtccttcg tatccaggcc aagcaaccgt ttcgtatcgc cagctatctg
     8161 tactaccttg agcgaagcag ggtcgacggc gagaagaaca ccatgaggtt gtatggaccc
     8221 cggaatatgg attggctccc gatcgcaatc ggtaagattg gagatcctcc ttgctgccgc
     8281 cagctccttc attccccacc agagttgttc agcgctctca actgtcgcgt gttggagcgt
     8341 atattctttt tcggccctgg ctatgacgat aatcaaattc tttcgcagtt gcaaagaagt
     8401 tggcctttgg cgctcgttct ctcgcgccaa aagccagtac atcaaatcaa atggtcaagg
     8461 tccagctagt agagttcggc ctaacggttc ataacgaggg acttaataca atagatttat
     8521 attaacgata ttagacatag gaaaaaggaa tgtcggcgaa gcggggggtt aaaacggcgc
     8581 cgtcttgact gatccttttc caagctcgtg cgtatgttct gcgattttac tttgtaaccg
     8641 tgccatcaat gcaaaggtcg gtcgcggcgt tcctgcgttc ccagcaggtc tgtgactgcg
     8701 cgaagcgaca ttacgcctct gcttcgaagt gcgcttgcat agtcgagccg agcaggccta
     8761 tcagtaaacc caacgcggcg gcgcaaaggc gtggtcggcg ccttcattat ggcgatcggc
     8821 gtcgctttat ctgcggtttt ttgttggagg caaataatgg gtcagcccgc tcaagcagtt
     8881 caaaaagcaa atggaagtat agcgcttccg gtcctcaagc tgttgctgat cgttgcagcg
     8941 ctcgtagcga tcggcttttc aatgatagtc atcgacctac cgaataaaaa ctatctatgg
     9001 ataggcgctg aagttgtggt tgctgttctc tttattatgt ctggcgtcag ctatcttaag
     9061 caaatttttg gaggcaagcg ctgaaataag cggcttgccg aatttgcata gacgcggtct
     9121 cgcgggactg gcggctcact cggatatctg gaacgagtcc acgaagcggt ccacttgctg
     9181 ctggccgttc ggcttcaccg ac
//
LOCUS       DATMGX010000767         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_326760, whole genome shotgun sequence.
ACCESSION   DATMGX010000767 DATMGX010000000
VERSION     DATMGX010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326760"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..>2412
                     /locus_tag="VNR65_12630"
     CDS             326..>2412
                     /locus_tag="VNR65_12630"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HWJ19561.1"
                     /translation="METLEISSVPPAEDSDDEMSAPGLRKNFERHLRRTLARDRFSAT
                     DRDRYYALALAVRDHLIERWIATQQEHHRKNVKRVYYLSLEFLIGRMMGNNVINMQME
                     DICRKAMAQEGLDWEDLREYEIDAGLGNGGLGRLAACFMDSIATLDIPAVGYGLRYDF
                     GIFKQNIKNGYQVEQPDEWLKHRYPWEIPHPEFSFDVQFEGRVEARYGPKGPEYHWLD
                     TKTVIGIPYDLPVVGYGGGTVNTLRLWSAKSAEEFDLDDFNRGEYVEAVANKVLAENL
                     TKVLYPNDSVSAGKELRLKQEYFFVSCTIQDIVRRFRADGNSWESFPDKVFVQLNDTH
                     PALVVPELMRLLMDGEGLEWDEAWQISTASTGYTNHTILPEALEKWPISMMERLLPRH
                     LQIIYEINSRFLREVAIHYPYDADRLRRMSIIEEGEPKHVRMAHLAIVGSSSVNGVSQ
                     LHTNLLQTKVLRDFAEYWPSKFNNKTNGITPRRWLLKANPSLAHLITEAIGDRWIRDL
                     EELHKLERFADDSAFRESFRAAKHQNKQALARIVDSTLGVSISPESLFDVQVKRLHEY
                     KRQLLLVLYIIVLYNRLKSDPVLDIVPRTFIIGGKAAPGYAMAKLIIKLIHQVAGVIN
                     SDAQVNEKMKVVFLPNYRVSLAERIIPAANLSEQISLAGTEASGTGNMKFMLNGAVTI
                     GTLDGANVEILEEV"
BASE COUNT          561 a          682 c          675 g          494 t
ORIGIN      
        1 atctgaaaca gcagcatgtc ggctattgcc actcgtcctt tttggggatg gcatacttca
       61 gcgggacgaa agatactgtt tgccggagac ataagtgtct gctccccggc tttttgctgc
      121 acaacgcgaa gaaacacacg cgacttggct cgactcaggg gcgcagcacg tccccaacgc
      181 gcagtgctat ccgagcaaca ttagtgtaag cttaacagca gcggctgctc tttataacgc
      241 agcacagctt tcgagtgact agccactcag tcaacgcggc tgggacgcgc aagcgaagcg
      301 gtaaccggga gattcaaggt tcaaaatgga aacactcgag atcagcagcg taccgccggc
      361 cgaggacagc gacgacgaaa tgtcagcccc cggtctcagg aagaatttcg agcgccatct
      421 ccgtcgcaca cttgcaaggg accgcttcag cgcgaccgac cgggaccggt actacgcgct
      481 tgcgcttgcg gtgagggatc acctgatcga gcgatggatt gcgacccagc aagagcatca
      541 ccggaagaat gtcaaaaggg tctattacct ctcgctcgag ttcctgatcg ggcggatgat
      601 gggcaacaat gtcatcaata tgcaaatgga agatatctgt cgcaaggcca tggctcaaga
      661 aggactcgat tgggaggatc tcagagaata cgaaatcgac gcgggcctcg gcaatggcgg
      721 cctcggccgg ctcgctgcgt gttttatgga ctcgattgcc actttggata ttcccgctgt
      781 cggctatggc ctccgatatg atttcgggat tttcaagcag aacatcaaaa acggttacca
      841 ggtcgagcag cccgatgaat ggctgaagca ccgctatccc tgggagattc ctcatcctga
      901 gttctcgttc gacgtccagt tcgaaggccg agtagaggcc agatacggtc cgaaagggcc
      961 ggagtaccac tggctcgaca caaaaaccgt tatcgggatt ccttacgatc tccccgtcgt
     1021 gggctacggg ggaggcaccg ttaacacgct gcggctgtgg tcggccaaat cagcggaaga
     1081 attcgacctg gatgacttca accgcggcga gtacgttgaa gcggtcgcga acaaggtgct
     1141 ggccgagaac ctgaccaagg ttctttatcc gaacgactcc gtctctgccg gcaaggaact
     1201 ccgtctcaag caggaatact tcttcgtttc ttgcacgatc caagacattg tgcggcggtt
     1261 cagggcggac ggcaactcat gggaaagctt tccggataag gttttcgtgc aactcaacga
     1321 cacgcacccg gcgcttgtcg ttcccgagct catgcgcttg ctcatggatg gcgaaggatt
     1381 ggagtgggat gaagcctggc agatttcgac cgcttcgacg ggctatacca accatacgat
     1441 cctgcccgag gctctagaga aatggccaat ctccatgatg gagcggctgc ttccgcgtca
     1501 cctgcagatc atctatgaaa tcaattcccg ttttctccgc gaagtcgcca ttcactatcc
     1561 ttacgacgcc gaccgcctcc gccgcatgag catcatcgag gagggtgagc cgaagcatgt
     1621 ccgcatggct catctggcga ttgtcggatc gtcctcggtc aacggcgtct cgcagcttca
     1681 caccaacctt ctgcagacca aggtactgag ggatttcgcc gaatattggc cgtccaagtt
     1741 caacaataaa acgaacggaa ttactccaag gcgctggctc ttgaaagcca atccgtcact
     1801 cgcgcatctg atcaccgaag cgattggcga ccgatggatc agggaccttg aagagctcca
     1861 taaacttgaa cggttcgccg atgactcggc ctttcgcgag agctttcggg cggcgaagca
     1921 ccaaaacaag caggcgctcg cgcggattgt cgacagcact cttggcgttt ccatttcgcc
     1981 tgagtcgctg ttcgatgttc aggtgaaacg gctccacgaa tacaagcggc aactgctgct
     2041 cgtgctctac atcatcgttc tctacaaccg gctcaagagc gatcccgtcc tggacatcgt
     2101 tccaagaacc ttcatcatcg gcggcaaagc ggcgcccggc tatgcgatgg ccaagctgat
     2161 catcaagctc attcatcaag tcgccggcgt catcaatagc gacgcccagg tgaatgagaa
     2221 gatgaaggtg gtgtttctgc ccaactacag ggtttcgctg gcggagagga tcatcccggc
     2281 cgcgaacctg agcgagcaga tttcgcttgc cggaaccgag gcgtccggta ccggcaacat
     2341 gaagttcatg ctcaacgggg cggtgaccat cggcacgctc gacggcgcga acgtggagat
     2401 cctcgaagaa gt
//
LOCUS       DATMGX010000768         2383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_219825, whole genome shotgun sequence.
ACCESSION   DATMGX010000768 DATMGX010000000
VERSION     DATMGX010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219825"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VNR65_12635"
     CDS             <1..278
                     /locus_tag="VNR65_12635"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HWJ19562.1"
                     /translation="IGFSPAYVAASVATGVMISTFVGMAMQSLRRGLTMLGIFGVLFG
                     LLYMILQLEDYALLAGSIAGFVLLTATMFATLRVNWSGDTGEAHTAS"
     gene            complement(424..1299)
                     /locus_tag="VNR65_12640"
     CDS             complement(424..1299)
                     /locus_tag="VNR65_12640"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HWJ19563.1"
                     /translation="MLAAAIIVFREVIEAGLIVGIVLAATRGVRSRAVYVLGGIAAGV
                     LGASLLAAFAGTLSNALEGVGQEVFSASILGVAVLMLGWHNVWMAQHGRELAAELTAA
                     GQAVVAGSKTLTALAIVVAIAVLREGAEVVLFLYGVVIANGESGWGLFAGGMLGLAAG
                     AAMSALTYAGLVKIPPRYLFSVTSMLIAFLAAGMASQCVAYLERAELLDLWSTTLWDT
                     SWLLPSKSLAGRVLHTLIGYDDRPSIMQAVAYIGTLATILVAMRVFAPAKPKPKPKLS
                     DASAPSKPRVATERA"
     gene            complement(1309..1650)
                     /locus_tag="VNR65_12645"
     CDS             complement(1309..1650)
                     /locus_tag="VNR65_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWJ19564.1"
                     /translation="MPFTRLLIVLLTAAFASYPVSVRADDPFPIGLSLKDHVFDPAEL
                     KAPAGRDIAITLKNEDESFEEFDSDALRTEKIVTAKGKVTIKLKPLAAGKYPFRGEFH
                     DATAQGVLIVE"
     gene            2030..2293
                     /locus_tag="VNR65_12650"
     CDS             2030..2293
                     /locus_tag="VNR65_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19565.1"
                     /translation="MFIELHDTGGNITLICVEDIQRVRKGAHGGSDIFMNELEGWVQV
                     AESVAAVRDLIVKAGDIVTASHVEGGAKPIRLQPVDSPRDKDQ"
BASE COUNT          470 a          714 c          709 g          490 t
ORIGIN      
        1 atatcggctt ttcgcccgcc tatgtcgccg cctctgtggc gaccggtgtc atgatttcca
       61 cctttgttgg catggccatg caaagcttga ggcgcggtct taccatgctc ggcattttcg
      121 gcgtgctgtt cggtctactc tacatgatcc ttcagctgga ggactatgcg ctgctcgcag
      181 gctccatcgc cgggttcgtt ttgctcaccg cgaccatgtt cgccactctg cgggtgaact
      241 ggtccggcga caccggcgaa gcccataccg cgagttagca tgctgcagca aaggagggcc
      301 ggagcgttct atgcccggcc gtcaatcttt tccaaacggt atcacgtccg caagcaccct
      361 tgcgttctat cagacgcgag catcactatg attgtacccg gggacgctgg aacgaaccgt
      421 caatcaagcg cgctcggtcg cgacgcgcgg tttcgagggt gcagacgcgt cgctgagctt
      481 cggcttcggc ttcggcttcg ctggcgcgaa cacgcgcatg gccacgagga tcgtcgcaag
      541 cgtcccgatg taagctacgg cctgcatgat ggacggccgg tcgtcatagc cgatgagcgt
      601 atgcaggacg cgccccgcga gactcttcga cggaagaagc caggatgtat cccaaagcgt
      661 tgtgctccag aggtcgagga gctcggcgcg ttcaaggtag gcaacgcatt gcgatgccat
      721 tccggcggcg aggaacgcga tcagcatgct cgtcacactg aacagatagc gaggggggat
      781 cttcacgagc cccgcatagg taagagcgct catcgcggct ccggcagcga ggcccagcat
      841 gccgccggca aatagacccc agccgctttc tccgttcgcg ataacgaccc cgtagaggaa
      901 caaaacaacc tccgcgccct cgcgcaaaac agcgatggcg acgacaatgg cgagcgcggt
      961 caatgtcttg ctgcctgcaa ctaccgcctg cccggcagcc gtaagctcgg cggcgagttc
     1021 ccttccgtgc tgcgccatcc agacattgtg ccagccgagc atcagcacgg ccacgccgag
     1081 gatggaggcg ctgaagactt cctgcccaac accttcgagt gcgttcgaaa gcgtgccagc
     1141 gaaagcggcg agcaaggacg cgccgagcac gcctgcagcg atgccgccaa ggacatagac
     1201 ggcccgcgac cgtacgcccc ttgtagccgc aagcacgatg ccgacgatca gcccggcttc
     1261 gatgacctca cgaaaaacga tgatggcggc ggcaagcata gttcgctcct attcgacaat
     1321 cagcacgccc tgtgcggttg cgtcgtgaaa ttcgcccctg aatgggtatt tgcccgcggc
     1381 gagcggcttc agcttaatcg taacctttcc ttttgcggtt acaatcttct cggtgcgaag
     1441 cgcgtcgcta tcgaactcct cgaagctctc gtcctcgttc ttgagcgtga ttgcgatgtc
     1501 tcttccggcg ggagctttga gttcggcagg atcgaacaca tgatccttga gactcagccc
     1561 aatcgggaag ggatcgtcgg cgcggacgct tacggggtag ctggcgaacg ctgcggtgag
     1621 gaggactatc aggaggcggg tgaatggcat tataaagcgt tttcgttgtc ttggccgccc
     1681 gcgccgcctc ctgaacatat ctgttgctga gcgactatcc tggcgcgaat agaagtaaaa
     1741 gatcggtaac gttggtattt tctagtgtcg gcgaaccagg agcgcaatct ttttcttttt
     1801 taattagagc ggttctaaaa tgcgcgtttg gcaaccttag ggtttttgtg agactactcg
     1861 gttatattgc gttcggcttc tggaaacctg ccgtatcgcg ccttcgcgcc ccgctctagg
     1921 cccctcaaaa aaatacgcct catttcccgc ggcagtgcta taagtcgggg ccttcggcgg
     1981 agcgagcctg gcaagcgagc tgttatgcct gttcgggagg atcacatcca tgttcatcga
     2041 gcttcacgat accggtggga acatcacgct gatttgtgtc gaggacatcc aacgcgtgcg
     2101 aaagggcgcg catggcggct ccgacatctt tatgaacgaa ctcgaaggct gggttcaagt
     2161 ggctgaaagc gtcgcggcgg ttcgcgatct catcgtaaag gcgggcgata tcgtcacggc
     2221 ttcccacgtt gagggtggcg cgaagcccat ccgcctgcag cccgtcgata gcccgcgcga
     2281 taaggatcag taaaacgccg cccgcagggc acagcgggca gcgcttccgg caagtctcgg
     2341 caatttaccc gttccagccg ccgtgatgat ggccccagcc gcc
//
LOCUS       DATMGX010000769         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_360188, whole genome shotgun sequence.
ACCESSION   DATMGX010000769 DATMGX010000000
VERSION     DATMGX010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360188"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VNR65_12655"
     CDS             complement(<1..758)
                     /locus_tag="VNR65_12655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWJ19566.1"
                     /translation="METHELDQSATGAVRADAAANRVSRRDTSTPANSEVPFAVEEIF
                     FSRTNEKGHILFGNSVFQKISHYSWDELCRRPHNIIRHPDMPRAVFWLLWDTIEKGEP
                     VGAYVKNMAKDGRYYWVFAIVTPIQGGYLSIRIKPTSPLLPLIEREYAALLAAEKAHR
                     MKPLESARILLARLSELGFPDYGAFMATALSHEIKARDEKLGRTLDKATVHFDGLAKE
                     AGALLKYAEGAVSDHTAHRYVPFNLRVHAAQLGEA"
     gene            1393..>2264
                     /locus_tag="VNR65_12660"
     CDS             1393..>2264
                     /locus_tag="VNR65_12660"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HWJ19567.1"
                     /translation="MFRCVPGWTAIFLTLALVVAIQDSDRAAAQAPHDHDAQTGGAPP
                     SQSSGPTPSPAGATVYFVDAKNGATLQTKTILHFGLRNMGVAPAGVVKSNSGHHHLLI
                     DADLPPLNQPIPNDPQHLHFGGGQTEAEVVLPPGHHTLQLLLADKDHIPHSPPVMSDR
                     IMVDVVDSSQPASVAGREPTPQGARVYFEFPTSGACIQQKFVVKFGLSGMGVAPAGFE
                     KKNTGHHHLVIDSDLPKFDQPIPNDDNHLHFGTGQTEAEVTLQPGPHKLQLLFADARH
                     TPHDPPLYSRPISV"
BASE COUNT          476 a          664 c          627 g          497 t
ORIGIN      
        1 gcttctccga gttgcgcggc gtgcactctg agattgaagg gcacatagcg gtgagccgtg
       61 tgatcggaga cggcgccctc ggcatatttg agcagcgccc cggcctcttt cgcaagccca
      121 tcgaagtgaa cggtagcctt atccaaggtt cggccaagct tttcatcgcg cgcttttatc
      181 tcgtggctga gcgcggttgc cataaacgct ccatagtccg gaaagccgag ttctgaaagg
      241 cgcgcaagca atatgcgcgc gctctcgaga ggtttcatgc gatgggcttt ttccgctgcg
      301 aggagcgctg cgtattcgcg ctctatcaga ggcaagagcg ggctggtggg cttgatgcgg
      361 atggaaaggt agcccccttg gatcggcgtt acgatcgcaa aaacccagta atagcggccg
      421 tctttcgcca tgttcttgac gtaggcgcca accggttccc ccttttcaat cgtgtcccat
      481 agaagccaga agactgcccg gggcatgtcc ggatgccgga tgatgttatg cggtcttcgg
      541 caaagctcat cccaggaata gtggcttatc ttttggaaga cgctgtttcc gaaaaggatg
      601 tgccctttct cgttggttct ggagaagaag atttcttcga cggcgaaagg aacttccgag
      661 tttgcgggcg ttgacgtatc ccgtcgcgaa acgcggttgg ccgccgcgtc cgcgcggacc
      721 gcccccgtcg cgctttggtc tagctcgtgg gtttccacct tcgccaagtc cccgtctgat
      781 aaatacgaaa gccacttgtg cagaggcgtt cccaattaag cgtgcgcctc tttccgccag
      841 gaagctatcc cgggagcgac gcgaaaataa tgagaaggcg catcacacaa ccagaagctg
      901 tcacaccttg cttaaagccg cgttagcgaa agcggcaggg ctcgcggcct cacaaacata
      961 aagccttgcc cggaccaaag tccccggcgg cgttatccgt ctttgcgcca gacatcttac
     1021 cagttttgct cactaacaga caggttgcat cagttatgca atttcacgac gaggccggcg
     1081 aaccggtggc atcgcttgag ttcgtcaaag gagccgcccg tgaacgcgtg caatcgcaga
     1141 tgctcgagca gtctcgcggt ttgctccccg aaggcgcaat cgctggtcag gggctaacgg
     1201 ttccggcaga cctatcgcgg ccaagaagaa caaaagaggc gggtcgaaaa atcatgatta
     1261 taccatccgc caggggaagg gcctcggtca ccgctatcgt gtcaactcaa tgcaatactt
     1321 ctcgaaaaat tgggttgtta ggctttgcag cgcgatgcaa tctgaacatg gttttgcttt
     1381 cggagaaagt atatgtttag gtgtgtaccg ggatggactg ctattttttt gacgctcgcg
     1441 ctcgttgtcg caatccaaga tagcgataga gctgccgctc aagcgcctca tgatcatgac
     1501 gctcagaccg gcggcgcacc gccctcgcag tccagcggac caaccccctc gcctgctggc
     1561 gcaaccgttt attttgtcga cgccaagaac ggcgccaccc ttcaaacaaa gacgatcctg
     1621 cacttcggtt tgagaaacat gggcgtcgcg cccgccggcg tcgtcaagtc caactcggga
     1681 caccaccacc ttttgatcga cgcggatttg cctcccctca accagcccat tcccaacgat
     1741 ccgcagcacc tccatttcgg cggcggccag acggaggctg aggtcgtcct gccccccggc
     1801 catcatacgc tgcagcttct ccttgcggac aaggaccaca ttcctcatag ccctccggtt
     1861 atgtcggacc ggattatggt cgatgtcgtt gatagcagtc agcctgcttc tgtagcggga
     1921 cgtgaaccga ctcctcaagg tgcgcgtgtg tatttcgagt ttccgaccag cggagcgtgc
     1981 atacagcaaa agtttgtcgt gaagtttgga ttgtcaggta tgggcgttgc cccggccgga
     2041 ttcgaaaaga agaacacggg tcatcaccac ctggtgattg actcggacct gccaaagttc
     2101 gaccagccca ttccgaacga tgataaccac ttgcactttg gcaccggtca gaccgaggcg
     2161 gaggtcacat tgcaacccgg acctcataag cttcagcttc tgttcgccga tgcgcgacac
     2221 acaccgcatg atcccccgct ctactccagg ccgatttcgg tgtt
//
LOCUS       DATMGX010000770         2909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_414377, whole genome shotgun sequence.
ACCESSION   DATMGX010000770 DATMGX010000000
VERSION     DATMGX010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414377"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VNR65_12665"
     CDS             <1..462
                     /locus_tag="VNR65_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWJ19568.1"
                     /translation="IAIFRQGDFFGEGCLGAPSLRMSTATAVHPATIARVEKPIIVRI
                     IHEDPVFARLFIAHLLSRVARIQDDLLDQLFNSSEKRLARTLLLLAGLGTQSKPYSAD
                     LKVSQGTLAEMVGTTRSRVSFFMNRFRKMGLINYNGSVRVHKELLTFLLHE"
     gene            complement(546..1919)
                     /gene="mtaB"
                     /locus_tag="VNR65_12670"
     CDS             complement(546..1919)
                     /gene="mtaB"
                     /locus_tag="VNR65_12670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HWJ19569.1"
                     /translation="MATYHVENFGCRATQADGAAIERQLLGRGLERASAAVEAEVVIL
                     NTCTVTASADQDVRAAIRRIHRENPAARILVTGCYAQRAPEELSVLPGVSLVVGNSHK
                     HDLASYAAPLASDGGFVALDQVGLNPASTVIVGDIFAHTELMAAPVFEAGSFEKTRPN
                     LKVQDGCNNRCSFCIIPSVRGRSRSLKLEQILREVDGLVANGYREIVLSGINLGRWGR
                     ELTPRMNFAAMLRAILERTAIEKVRISSVEPMDWTNEVIELVASSPRIAKHAHVPLQS
                     GSDRILRKMHRKYRPWHYADRIERIRRAMPDAAIGADVMVGFPGETDDDFEQTRALTE
                     RLPFTYLHVFTYSSRPGTPSAEMPEQVPVHIARERNRVLRELAVQKKREFMQSFVGRE
                     LQAITLTHYDGECTEALTDNYLKLKLRGKHEANRWVRSLIEAVHSDALMGLLAVPASA
                     IPAGAPA"
     gene            2108..2572
                     /locus_tag="VNR65_12675"
     CDS             2108..2572
                     /locus_tag="VNR65_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ19570.1"
                     /translation="MKRRILLVDDELAILLTLRAILEMNDFEVDTASSAKEAEGKLKN
                     GVFEMVITDMRMKTETAGYDVIRAARQLAYNPATAILTAYPSLGNEWQSHGAQSLLVK
                     PVNTAELLRQIEALLISHQDHKMQAASANARKVPNPNNSKKRRPATAGKKAM"
     gene            2663..>2909
                     /locus_tag="VNR65_12680"
     CDS             2663..>2909
                     /locus_tag="VNR65_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19571.1"
                     /translation="MMAKTKSLTAHPSVDEALSGLTIPITEIVAVGGGETKGTQRMRR
                     ALSNLGWKKFEFVIRKIINGVERESTSHEIDHVKEFDG"
BASE COUNT          640 a          838 c          858 g          573 t
ORIGIN      
        1 atcgctattt ttcggcaagg cgactttttc ggtgaaggct gtttaggagc gccgtctttg
       61 cggatgtcca ccgcgaccgc ggtacacccg gccacaattg cgcgagtgga aaagccgatc
      121 atcgttcgca tcatccacga agatcctgtg tttgcaaggc tatttatcgc gcacctactt
      181 tctcgcgttg ctcgaattca agacgacctc ctcgaccaac tcttcaattc cagcgagaag
      241 cggctggcgc gaactcttct gttgctcgcg ggcttgggga cgcaatccaa gccatattcc
      301 gccgacctga aggtgagcca gggaaccctg gccgagatgg ttggcaccac ccggtcgaga
      361 gtaagcttct ttatgaaccg gtttcgcaag atgggcctca tcaattacaa cggcagcgtg
      421 cgggtgcaca aggaactgct caccttcctt ctccacgaat aggcgccaca cctcgcgtgc
      481 gctccgctca aggaaaaagc gaaagtgttt aaattccgac cagttttgag acgagcaaga
      541 gctcgttagg cgggagcacc ggccggaatt gccgacgccg ggactgcaag caagcccatc
      601 agcgcatcgg aatgcaccgc ttcgatcagg ctccgcaccc accgattcgc ttcgtgcttg
      661 ccgcgcagct tgagcttcag atagttgtcg gtaagcgcct cggtacattc gccgtcgtaa
      721 tgcgtgagtg tgatcgcttg tagttcgcgg ccgacaaacg actgcatgaa ctcgcgcttc
      781 ttctgcaccg ccagctcgcg cagcacgcga ttgcgttcgc gcgcaatgtg taccggaacc
      841 tgctccggca tctcggcgga cggagttccc ggacgcgacg aatacgtaaa cacgtgcaga
      901 taggtgaacg gcaggcgctc ggtgagtgcc cgcgtctgct cgaagtcgtc gtcggtctca
      961 ccgggaaagc caaccattac gtcggcgcca attgcggcat cgggcatggc ccggcggatt
     1021 cgctcgatgc gatcggcata atgccagggg cggtacttgc ggtgcatctt gcgcagaatg
     1081 cgatcgctgc ccgattgcaa cggcacgtgc gcgtgcttgg cgatgcgcgg tgacgacgcg
     1141 accaactcga tcacttcgtt cgtccagtcc ataggctcga cggagctgat gcggaccttc
     1201 tcgatcgccg tccgctcgag gatggcgcgc agcatggcgg cgaaattcat tctcggcgta
     1261 agttcgcgtc cccagcgccc caggttgatg cccgacagca caatctcgcg atagccgttg
     1321 gcgaccaacc catcgacttc gcgaagtatc tgctccaact tcaacgaccg gctgcggccg
     1381 cgcaccgacg ggatgatgca gaacgagcag cgattattgc agccgtcctg cactttgaga
     1441 ttgggacgtg tcttctcgaa ggagccggct tcaaaaactg gagcggccat caactcggtg
     1501 tgagcgaaga tgtcgccgac gatcacggtt gacgcgggat tcaggccgac ttgatccagc
     1561 gctacgaatc caccgtcgct tgccagcggg gcggcataac ttgccaggtc gtgtttgtgc
     1621 gaatttccca cgaccagact gacgccgggc agcaccgata gctcctcagg cgcgcgctgg
     1681 gcgtagcatc cggtaaccag gatcctggcg gcgggatttt cgcgatgaat gcggcggatg
     1741 gcagcacgca cgtcctgatc ggcagatgcc gtgacggtgc atgtgttcag gatgacgacc
     1801 tcggcctcta cggcggcgga ggcacgctcc agaccccggc cgagcaattg gcgttcgatg
     1861 gcggcgccat cggcctgggt cgcgcgacat ccaaaattct caacatgata agtcgccacc
     1921 aagctattat actggcgctt acttcttaag catgttgagc atggcagcgc gtccggcgtg
     1981 gtggaagggc aaaataatta gtgggacgcg cacggaatga gaatgcggtt aggagcaact
     2041 tggcggcagg aacttgcatt acacccgccg cacgttgatg atgagacttt ccgcgtggag
     2101 ttccccgatg aaacgtcgca ttctgttagt tgacgatgag cttgcaattc ttctcaccct
     2161 gcgcgcaatc ctcgaaatga acgattttga agtggacacc gcctcctccg ccaaggaagc
     2221 tgaggggaaa ctgaagaacg gcgtcttcga gatggtgatt actgatatgc gcatgaaaac
     2281 ggaaactgcg ggctacgacg tgattcgggc ggcgcgtcaa ttggcttaca acccagccac
     2341 cgcgatactg actgcatacc cgtccctggg caacgaatgg cagagccatg gagcgcaatc
     2401 gttgctggtg aagccggtca acacggcgga actgctgcgc cagatcgaag cgcttttgat
     2461 cagccatcag gaccacaaaa tgcaagcagc cagtgcgaat gccaggaaag ttcccaaccc
     2521 caataactcc aagaaaaggc gccccgccac ggcgggcaag aaggccatgt agcattaccg
     2581 ggaagttcgg ctgtccagcg ctgcatctcg tgatcggtct ggaacaggct gcgggaaaat
     2641 ccgaaaagca ggtccgctgc tcgtgatggc aaaaacaaag agcttaacgg cgcacccttc
     2701 ggtagacgaa gcactcagtg gcctgacgat tccgataacc gagatcgtcg ccgtcggtgg
     2761 tggagagacg aagggcacac agcgaatgcg ccgtgccctc tcgaatcttg gttggaagaa
     2821 gtttgagttc gtcatcagaa agatcattaa cggagtggag cgcgaatcaa cttcccacga
     2881 aatcgatcat gtcaaggaat ttgacggca
//
LOCUS       DATMGX010000771         4015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_3690,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000771 DATMGX010000000
VERSION     DATMGX010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3690"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VNR65_12685"
     CDS             <1..844
                     /locus_tag="VNR65_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HWJ19572.1"
                     /translation="ESLRAHGIKRIYPFLGPMDREGWPGWRSKAGFVRYDPDRAARFL
                     AEIHRIAPEIRVIPWTGGLLDSDVNLKDLGQRKAFADHARRLVEAGADGVHLNIEPLS
                     SGTSDYVQLLREVKAAIGDRILSVAAYPPPIPGQEVEDTHWELRFLREVCRNANEIAV
                     MAYNTGLTSAPAFEKLVATWTEELAATLPPPRAGGCEWLMGVPAYDDDKDYHRPDVET
                     IEHSLNGIVAGLRAVKNSEHFRGVAIYASFTTDERKWAVFDRLWRGVDPASSPPPDPR
                     NTTE"
     gene            complement(857..2602)
                     /locus_tag="VNR65_12690"
     CDS             complement(857..2602)
                     /locus_tag="VNR65_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19573.1"
                     /translation="MVRGVRLLVRLGLAIAILSGVMAGQLTADTNPVIGLFLGYSRAV
                     NTIALSANGRQALSGGQDNVLRLWDIPTGLLLRTFDAQASRINAVAFSPDGHLILSGG
                     SDKLVRIWAVSTGQAVRTIEGHAGEVLSVAISPDGKRALSGSADKTVKLWDVETGDLI
                     RTFEGHSDEVLSVAFSSDGQKALSASKDKTLKLWDVETGQLLKTLEGHADAVTSAALS
                     ADGAVALSGSKDKTIKLWDLASGQPVKTFVGHSDEVLSVVLSSDGRVLSAGKDKTLKL
                     WDAKTGELIKTFEGHSDSVTSVGFSPDGQNALSGSWDKTLKLWDLKTGQLQTTIDLQS
                     ITFWPSGYRNFFSGVSATKRADPAQVEERLKEKGLKRGAPVFLRIFKGDLEVELWMKR
                     GDRYDLFATYPICAWSGQLGPKVREGDLQAPEGFYTIGKGQLNPNSKYHRAFNLGYPN
                     ALDRAHNRTGANLMVHGACASVGCIAITDAAIDELWLLVTAALDSGQERVPVHVFPFR
                     MTDERLAAFAWHPWADFWRDMKPAYDLFEERHVPPQVSVCNKRYSVQGRTAGGWTPVS
                     QSECPSRRGGSLPLP"
     gene            complement(2743..>4015)
                     /locus_tag="VNR65_12695"
     CDS             complement(2743..>4015)
                     /locus_tag="VNR65_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19574.1"
                     /translation="AEERRTQLVAITGIGAEKPNGIEDAKHEERSNTLSTLPEAKGSE
                     NELREREDLREEIRDLALRALANDPLNAGAYRALGEVISGADQVRLLMQKAFEHSRRE
                     SIAVFWLLNDSYYRNDFKATLHYSDILLRTRPELGTYIYSYLSLLSAEPEGRSLLADR
                     LASEPTWRKQFFQVFPRASKNADATLLVMTTLRDSGKPVLDKELAPYLNFLVSINRID
                     AAYNARLQFLAADQLEKIELLTNGRFERKPSGLPFDWKIDPGVNAIADFVPLGKKSTE
                     NAFHIKFSDGRIQFPQLSQVLFLPPGRYRLEGNLRGSISGKRGLRWQIRCASGAQKIL
                     GETEMLLGQSPQWRVFAFDAEVPEPSECAGEILRLIHDSRSASEELLSGEVWFSNLRL
                     THLHPVTALWSPVQTAEKGAFGMRSASDRAK"
BASE COUNT          807 a         1189 c         1143 g          876 t
ORIGIN      
        1 cgaaagcctc agagcgcacg gaatcaagcg catctacccg tttcttggcc cgatggatcg
       61 cgagggctgg cccggctggc ggtccaaggc gggctttgtt cgctacgatc ctgaccgtgc
      121 agcgagattt cttgccgaga tccatcggat cgcacccgaa atccgggtca ttccatggac
      181 cggtggcctc ctcgacagcg acgtaaacct gaaggacctt ggccagcgca aggcgttcgc
      241 agaccatgcg cgccgtctcg ttgaggcagg cgccgatggg gtccatttga acatcgaacc
      301 cctttcgagc ggaacatccg attacgttca gcttcttcgg gaggtgaagg cggccatcgg
      361 cgaccgcatc ctctcggttg cggcctatcc tccgcccata cccgggcagg aggttgaaga
      421 tacgcattgg gagctgcgct ttttgcgcga ggtttgccgg aacgcgaacg agatcgcggt
      481 catggcctac aacaccggtt tgacgtccgc gcccgccttc gagaagctgg tcgcgacctg
      541 gactgaggag cttgccgcca ctctgcctcc accccgagcc ggcggttgcg aatggctcat
      601 gggtgttcct gcttatgatg acgacaagga ttatcaccgg cccgacgtag agacgatcga
      661 gcacagtctc aacggcatcg tggcaggtct tcgcgccgtc aagaattccg agcactttcg
      721 aggtgtcgcg atctatgcgt ctttcacgac ggatgaacga aaatgggccg tcttcgatcg
      781 gctttggcgc ggcgttgacc ccgcgtcgtc cccgccgccc gatccgcgaa atacaacaga
      841 gtagctggtt ccctcgtcag ggaagaggaa gcgaaccacc cctcctcgat ggacattcgg
      901 actgcgacac gggtgtccag cctcctgccg ttcgcccctg aacgctgtag cgcttgttgc
      961 agacgctaac ctgcggcgga acatggcgtt cttcaaaaag atcataggcc ggcttcatat
     1021 cgcgccagaa atcagcccaa gggtgccagg caaaggccgc caaacgctca tccgtcattc
     1081 tgaagggaaa cacgtgcact ggcacgcgct cttgcccact gtcaagcgct gcagttacga
     1141 gcagccacag ctcatcgatt gccgcatcgg ttatcgcgat gcagccgacc gacgcgcatg
     1201 cgccatggac catcaggttc gcgccggtcc ggttatgggc ccggtcgagg gcattggggt
     1261 atccgaggtt gaaagcgcga tggtatttgc tgttcggatt gagctggcct ttgccgatcg
     1321 tgtaaaagcc ttccggggct tgcagatcgc cttcgcgcac ttttggaccg agctgccccg
     1381 accaggcgca gatgggatag gtcgcgaaca ggtcgtatcg gtcgccccgc ttcatccaga
     1441 gttccacctc aaggtcgccc ttgaagatgc gcagaaacac aggcgcgccg cgcttcaggc
     1501 ccttctcttt gagccgctcc tcgacttggg cggggtcggc tcgtttggta gccgaaactc
     1561 cagagaagaa gttgcgatag ccgctgggcc agaatgttat cgattgcaga tcgatcgtag
     1621 tttgaagctg acccgtctta aggtcccaaa gcttcagtgt tttgtcccag cttcccgaaa
     1681 gagcgttctg tccatcgggc gaaaatccaa ctgaggtgac gctatcggaa tggccttcga
     1741 aagtcttgat cagctctccc gtctttgcat cccagagctt cagcgtctta tccttgccag
     1801 cagatagcac gcggccatcg ctggacaaga caaccgacag gacctcatcc gaatgtccca
     1861 cgaaggtttt gacaggctgg ccgctcgcca aatcccaaag cttgattgtc ttgtccttgc
     1921 tgccggagag cgccactgcg ccgtctgcag agagcgctgc cgacgtcacc gcatccgcgt
     1981 ggccctcaag cgtcttgagg agctggcccg tttccacatc ccacagcttg agtgtcttat
     2041 ccttgcttgc cgagagtgcc ttctgcccgt cggacgagaa ggcaacggac agcacctcgt
     2101 cggagtgccc ctcaaaggtc ctgatgagat cgccagtttc cacgtcccac agtttgactg
     2161 ttttgtcagc gcttccggaa agcgctcgct tgccgtcggg cgagatggcg accgatagca
     2221 cctcgcccgc gtgcccttcg attgttctga cggcctgccc ggtcgagacg gcccaaatgc
     2281 ggacaagctt gtcgctgccg cccgacagga ttagatgccc atccggagaa aatgcgactg
     2341 cgttgatcct gctcgcttgg gcgtcaaagg ttctgaggag gagccctgtc ggtatgtccc
     2401 acagtctcag aacattgtcc tgaccgccgg agagcgcctg ccggccattt gcagagagtg
     2461 caatcgtatt gacggctctt gagtagccca agaagagccc gataaccgga ttcgtgtcgg
     2521 ccgtgagttg tccggccatt acgccagaca aaattgcaat cgccagaccc agccgtacca
     2581 agagtcgcac accgcgcacc atgtctcgtg cctcccagca acacgcggta tcatcttaaa
     2641 tgccaacttc gcaagaactc aggtctggat tcgttgcgcc ccaggatggt attggccacc
     2701 ataaccgcca tcaaagtgcg cggcaacgac cgaggccaag attcacttgg cgcggtcgga
     2761 ggcactccgc atgccaaaag cgcctttttc tgcggtttgg accggcgacc agagtgcagt
     2821 gaccggatgc aaatgggtca gacggagatt gctgaaccag acttctccgg acagaagttc
     2881 ctccgatgcg gatctggaat cgtgaataag ccgcagtatt tcgccggcgc attcagaggg
     2941 ttccggcacc tcggcatcaa acgcgaaaac ccgccattgc ggagactggc cgagcagcat
     3001 ttccgtctct cccagaatct tttgcgctcc ggaagcacaa cggatttgcc agcgcagtcc
     3061 gcgtttgccg ctgatcgatc cgcgcagatt gccctcaagc cggtaacggc ccggcggaag
     3121 aaacagaacc tggctgagtt gcggaaattg gatgcgccca tcgctgaact tgatatgaaa
     3181 cgcattttct gtgctcttct tcccgagcgg tacgaaatct gcaatcgcgt ttacgcctgg
     3241 gtctatcttc caatcgaaag gaagcccgct cggttttctt tcgaagcgtc cgttcgtcag
     3301 gagttcgatt ttttcgagct ggtccgccgc taagaattgc agcctcgcat tataggctgc
     3361 gtctattcga ttgatgctga ctagaaagtt gaggtaagga gcaagctcct tatccaatac
     3421 gggttttcct gaatctctca gtgtcgtcat gaccaggaga gtcgcgtccg catttttgga
     3481 tgcgcgcgga aagacctgga agaactgctt acgccaggtg ggttccgaag ccagccgatc
     3541 agcaagaaga ctgcggcctt ccggttcagc tgagagaagg gagaggtagc tgtaaatgta
     3601 tgtccccaat tctggccgtg ttcgaagcaa gatatcggag taatgaagtg tggccttgaa
     3661 atcattgcga taatagctgt cattgagcag ccagaacaca gcaatcgatt cgcgccgaga
     3721 atgctcaaac gctttttgca tgagcaggcg gacttggtct gcgccgctga tcacctctcc
     3781 cagtgcccgg tatgcccctg cgtttagtgg atcatttgca agtgcacgca gcgctagatc
     3841 gcgaatttcc tcgcgcaggt cttcgcgttc gcggagttca ttttcgcttc cctttgcttc
     3901 gggcagggtg gagagtgtat tggaccgttc ctcatgcttg gcatcctcga ttccgttggg
     3961 cttttctgcc ccgatgccgg tgatcgctac aagctgtgtc ctgcgctctt cggct
//
LOCUS       DATMGX010000772         2540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_135504, whole genome shotgun sequence.
ACCESSION   DATMGX010000772 DATMGX010000000
VERSION     DATMGX010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135504"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2540
                     /locus_tag="VNR65_12700"
     CDS             <1..>2540
                     /locus_tag="VNR65_12700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWJ19575.1"
                     /translation="VRPWRAANGSIGGILIFTEDVTARVEIERALRESREDLDHAQAV
                     ARMGSWRLDINRNKLTWSAETFRIFGISPETPLTYESFLAAVHPDDREQVDRSWKGAL
                     EGARYDIEHRIVVGTKLKWVRERAELEFDAAGKLLGGFGTVQDITDKKQIEWQLHESE
                     ERLRLSNEAGGIGTFTIDLESNCAIYSPELSAMLGFPGVSKAPVEVAFALVHRDDQAR
                     IRAAFLAALQAPGKGRVREEFRFVLPGGEVRWMRWNGRVDFREGPSGPTPSMMFGACV
                     DITGLKRTEMALRESEKLFRATFETAAVGVAHVAPDGSWLLANSRMGEILGYQAEELT
                     NKKFQDITHPDYLEADLVQLGRLLRDEIDNYGVEKRYLRKDGSIVWARLTVATVRKAD
                     RAVDYFISVVEDIDERKRAEEKLRQSEARYRMLHESLRDAFVQVSMDGRIVEFNDLYC
                     QMLGYSPEEIRGLTFQELTPERWHAFEDLIVREQIIPRGYSDIYEKEYRRKDGTIIPV
                     ELRAVLSRDGFGSDTMWAIVRDITERKRAERALRESREDLDHAQAVAHIGSWRIDVTR
                     NEVTWSAETFRMFRISPETPLSYESFLAAVHPDDRERVNNAWKAALGGALFDIEYRIM
                     AGAETKWVRARAELERDDTGAVLGGFGTVQDITDRKRAEEQLRESEGRFRGIFQHAGT
                     GIAILDLQGRFRSCNPAYSAMLGYTEDELRSLVFPYLIHPDDRDRNLAEVERLLAQEV
                     PAIEITNRYIGKDGQPLWVRKFVSLLRDASGKPTSFITLVTDITERKRNEEQIKLLMN
                     EVNHRSKNLLTLVQAIARQTVATTPDDFIDRFQKRIEALSASQDLLVR"
BASE COUNT          539 a          725 c          798 g          478 t
ORIGIN      
        1 aggtgcggcc ttggcgtgcg gctaatggga gcataggcgg gatcctgatc ttcaccgaag
       61 acgtgaccgc ccgtgtcgaa atagagcggg cgctgcgtga aagccgcgag gatctggacc
      121 atgcgcaggc cgtggcccgc atgggcagct ggcggctcga tatcaaccgc aacaagctta
      181 cctggtctgc agaaacgttt cgtatcttcg gcatttcccc cgagacgccg ctgacctatg
      241 agagcttcct ggccgccgtc catcccgacg accgggagca agtcgaccga agctggaagg
      301 gggccctcga aggcgctcgc tacgacatcg aacatcgcat cgtcgtgggc acaaagctta
      361 aatgggtccg ggagcgggcc gagctggaat tcgacgctgc cggtaagctt cttggcggtt
      421 tcggcaccgt gcaggatatc acggacaaaa aacagataga gtggcagctt cacgaaagcg
      481 aggagcggct gcgtctctcc aacgaggcgg ggggcattgg cacattcacc atcgatctcg
      541 aatccaattg cgcgatctac tcaccggaat tgtcggccat gcttggattt ccgggggtaa
      601 gcaaagctcc cgtcgaggtc gcgtttgccc ttgtgcaccg cgacgatcag gcgaggatcc
      661 gcgccgcttt cctggcggct ctgcaagcgc cgggcaaagg tcgggtgaga gaggagttcc
      721 gctttgtcct tccaggcggc gaagtccgct ggatgaggtg gaacggccgc gtcgatttcc
      781 gcgaaggtcc ttcgggcccg accccatcca tgatgttcgg cgcctgcgtg gacatcaccg
      841 ggcttaagcg caccgagatg gctctgcgcg agagcgaaaa actgttccgg gcgacgttcg
      901 aaactgctgc ggttggggtc gctcacgtcg cgcctgacgg ctcctggctt ctggcaaata
      961 gccgcatggg tgagatactt ggctaccaag ctgaggagct taccaacaaa aaattccaag
     1021 acattactca tcccgattac ctcgaggccg atctggtgca actcggacgc ctgctccgtg
     1081 acgaaatcga caactacggc gttgaaaagc gctacctgcg caaagatggc tcgatcgttt
     1141 gggcgagact gacggtggcc acggtgcgca aagcggaccg cgccgtcgac tatttcatca
     1201 gcgtcgtcga ggatatcgac gaacgtaagc gcgcagagga gaagctgcgc cagtccgagg
     1261 cgcgttaccg gatgctgcat gaaagcctgc gcgacgcctt cgtgcaggtc tctatggatg
     1321 ggcggatcgt cgaattcaac gacctctatt gccagatgct tggttactcg cctgaggaga
     1381 tccgggggct gactttccag gagctgacac cggagcgctg gcatgccttc gaagatctca
     1441 tcgtccgcga acagatcata ccgcgagggt actccgacat ttacgagaag gagtatcggc
     1501 gcaaggatgg gaccatcatt cccgtcgagt tgcgagccgt gctgtcgcga gatgggttcg
     1561 gatcagacac gatgtgggct atcgtgcgtg acataaccga gcgcaagcgg gcggagcgcg
     1621 ccctgcgtga aagccgcgag gatctggacc atgcgcaggc cgtggcgcac atcggcagct
     1681 ggcggatcga cgttacgcga aacgaagtca catggtctgc ggagacgttt cgtatgttcc
     1741 gcatctcgcc tgagacgcct ctaagctacg agagcttcct tgccgctgtg catcccgatg
     1801 accgcgagcg tgtcaacaac gcctggaagg cggctcttgg cggagcgctc ttcgacatcg
     1861 aataccgcat catggcgggc gcagaaacga aatgggtccg agcgcgggcc gagttggagc
     1921 gcgatgatac cggcgctgtg ctcggggggt tcggtacggt gcaggacata acggacagga
     1981 aaagagcgga ggagcagctt agggaaagcg aggggcgatt ccgcggcata ttccagcatg
     2041 ccggcaccgg catcgccatt ttagacttac aaggccggtt tcgctcctgc aatccggcct
     2101 actccgccat gttgggctac accgaggatg agcttcgatc gctcgttttc ccatacctta
     2161 tccatccgga cgatcgggac aggaatctgg cagaggtcga acggctgctt gcgcaagagg
     2221 tgccggcgat cgaaatcacg aatcggtaca ttggcaagga cggccaaccc ttgtgggtgc
     2281 gcaaattcgt ctccttgctt cgcgacgcct ccggcaagcc aaccagcttt atcacgcttg
     2341 tgaccgacat aaccgaacga aagcgcaacg aggagcagat caaactgctc atgaacgagg
     2401 ttaaccatcg ctcgaagaac cttctcaccc ttgtgcaggc gattgcgcgg caaaccgttg
     2461 cgacgacccc ggatgacttc atcgaccgct ttcagaaacg catcgaggcg ctatcggcga
     2521 gccaagatct gcttgtgagg
//
LOCUS       DATMGX010000773         5327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_248339, whole genome shotgun sequence.
ACCESSION   DATMGX010000773 DATMGX010000000
VERSION     DATMGX010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248339"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VNR65_12705"
     CDS             <1..556
                     /locus_tag="VNR65_12705"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWJ19576.1"
                     /translation="FGAHVSHRPQPLGEAFLTLLDRDQMRLLRLCATLEKIADGLPGT
                     GLHRRTGKVLAFLDKAFARHVFLHEKCLFPLISSLEDKKESVELILRELEFEHAADRG
                     LIVEITSAFMGRAGVETQMLGYLLRAFFENYRRHCSWERNVLHPIVRLHLAGGTPRKQ
                     HDALLRASMGANVSDAILKTAALC"
     gene            complement(586..4245)
                     /gene="putA"
                     /locus_tag="VNR65_12710"
     CDS             complement(586..4245)
                     /gene="putA"
                     /locus_tag="VNR65_12710"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HWJ19577.1"
                     /translation="MDAKPMDVLQEANNAQDRGEEKRGAIRAAYRVPEPVCVPPLVEE
                     ARLDPALSAKVQNLAHGLVNKMRAERGHAFGVEALMKEFSLSTPEGMALMCLAESLLR
                     IPDDETRDVLIRDKLSQGDWRAHVGASQSWFVNASTWGLLITGKLVATSSEHGIAASL
                     SRALARGGEPVIRKAMDLAMRLLGRQFVTGKTIEEALGHAKPFEERGFLYSFDMLGEA
                     ALTEEDAANYMDAYEHAINAIGSANEEPGIHESAGISVKLSALHPRYNYTQMERVKTE
                     LYPRLRKLALLSIEQGIGFNIDAEEADRLEPSLDLFEALANDSALGRWQGLGFVVQAY
                     QKRAPFVIDTLIDLAERTKRRLMIRLVKGAYWDSEIKRAQVDGLSGYPVYTQKSYTDV
                     CYLACAKRLLARRDAVFPQFATHNAQTLAAIYHMAGPEFRIGDYEFQCLHGMGETLYE
                     HVVGEKNLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSFVHQLVDERIPIEELIAD
                     PVAEARRQGGAPHPQIVLPGDLFAPERKNSKGLDLSDAGARGELERTVADSTGASYEA
                     EPILATPATTGQPWKAICNPANHADIVGHAVAATKMDVEAAMASAAAAAPRWANTSAG
                     ERAAILERGGDLLEHAMEQLSARLIREAGKSMPNAVSEVREAVDFCRYYAAQARNELS
                     APHTPLGPTVCISPWNFPLAIFTGQIVAALAAGNPVLAKPAEQTPLIAAEAVRLLHEA
                     GIPRDVLQLVPGRGSVIGAALVADPRTQAVVFTGSTEVARSIQGTLAERGNITLIAET
                     GGQNAMIVDSSALPEQVVGDVLASAFDSAGQRCSALRVLCLQDDIADPVIEMLKGAMA
                     ELKMGDPASLETDVGPVIDSSAQTALSAYVESKRSRILFQTPLAPSSTSGTFVAPTLL
                     RIDSISELTHEVFGPILHVIRFKRDDLPSLIDGINDTGYGLTLGIHSRIDETIDFIVN
                     RARVGNIYVNRNMIGAVVGVQPFGGGGLSGTGPKAGGPLYIHRLLREAPPARLEGERD
                     DKKLDHLDALAKWIEGGASGLLSEAEGAQLSETFGRYRKDSPLPVEMTLKGPVGEDNR
                     LRFLPRGTILGIVNSIPEALHQFGAALATGNRFLLQDDEAARRVLDGLPKPLRARIAS
                     AAAWDTSSFDAVLLSDKARLQEIAQSLARCRGPIIPVLHGNPKYSLEMLVKERTVSVN
                     TAAAGGNASLMTIGK"
     gene            4407..5084
                     /locus_tag="VNR65_12715"
     CDS             4407..5084
                     /locus_tag="VNR65_12715"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ19578.1"
                     /translation="MIPKPTTKDQAAPKRGAPDLSERRRPTQDERRAAILAAALDVFA
                     ENGFAAARLDDVAQKAGVAKGTLYLYFPDKQALFEALLQGLAGPVLQRFEALSADPAL
                     PASAILGGILAVFQTEIIGTNRERLMRLIIAEGPRFPKIAEFYHREIICKGREIIRAI
                     AARGYERGELPSDAMARFPQLFFAPLLTAVLWRSLFSQFDPLDVNAMIECHRKLMLGL
                     GDEEIPK"
     gene            5081..>5327
                     /locus_tag="VNR65_12720"
     CDS             5081..>5327
                     /locus_tag="VNR65_12720"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HWJ19579.1"
                     /translation="MKRPPKAVLIALLIAGGLAGAWWYKTRHVGTGELVLQGNVEVRQ
                     VNLGFKVPGRIETLNVDEGDRVRTGQVLASLDKIYFQD"
BASE COUNT          977 a         1622 c         1664 g         1064 t
ORIGIN      
        1 cttcggcgct catgtgagcc acaggccgca acctttgggg gaggcgtttc taacgcttct
       61 ggatcgggac caaatgcgcc tgctgcgact ttgcgccacc ctggaaaaga ttgccgacgg
      121 ccttcccggt acggggctgc atcgaaggac cgggaaggtt ctggcctttc tcgacaaggc
      181 cttcgcaagg catgtcttcc ttcatgagaa gtgcctcttt ccgctcatca gcagcctgga
      241 agacaagaag gaatccgttg agctcatcct tcgcgagctg gaattcgagc acgccgccga
      301 tcgtggcctg attgtggaaa tcacgtccgc tttcatgggc cgcgctggcg tcgaaacgca
      361 gatgctgggc tatctgctgc gggcgttttt cgagaattac cgccgccatt gttcgtggga
      421 gcgcaacgtt cttcatccca tcgtgcgcct gcatcttgct ggcggcacgc cccggaagca
      481 gcatgacgcg ctgttgcgtg cctccatggg cgcgaacgtc tcggacgcca tcctcaagac
      541 agcggctctc tgctgaaccg cgttttcgct tgccaatgga cgaggtcact ttccaatcgt
      601 catgaggctt gcgttgccgc ccgcggcggc ggtgttgacg gagaccgttc gctctttcac
      661 gagcatctca aggctgtatt tcggattccc atgaagaacc gggatgatcg ggcctcggca
      721 acgcgccaag ctttgcgcaa tctcttgaag ccttgcctta tcggagagca gcactgcatc
      781 gaagctggag gtatcccatg cggcggcgct tgcaatgcga gcgcgtaaag gtttcgggag
      841 gccatcgagc acgcgccttg ccgcctcatc gtcttgcagc agaaagcgat tgccagtcgc
      901 aagcgcagcc ccgaattgat gcagcgcctc ggggattgag ttcacgatgc cgaggatggt
      961 tccgcgcggc aggaagcgga gccggttgtc ttcaccgacc ggccctttga gcgtcatctc
     1021 aaccggcaga ggggagtctt tccggtagcg gccgaaggtt tcggaaagct gcgcgccttc
     1081 agcttcgctc aagaggccgc tcgcgccgcc ctctatccat tttgcaagcg cgtccagatg
     1141 gtcgagtttc ttgtcatccc gctcgccctc aagccgcgca ggcggcgctt cccgaagcag
     1201 ccgatgaatg taaagaggcc cgccagcctt cggccccgtt ccggaaaggc ccccgccgcc
     1261 aaacggctga acgccgacca ccgcgccgat catgttccgg ttcacataga tgtttccaac
     1321 gcgggcgcgg ttcacgatga agtcgatggt ttcgtcgatg cgagagtgaa tgccgagcgt
     1381 aagtccatag ccggtgtcgt tgatgccatc gatgaggctt ggcaggtcgt cgcgcttgaa
     1441 gcggatcaca tgcaggatcg ggccgaagac ttcgtgcgtg agttcgctaa tcgagtcgat
     1501 gcgaagcagg gtgggggcta cgaaagtgcc gctcgtggag gaaggagcga gcggcgtctg
     1561 aaacaggata cggctgcgct ttgactctac ataggcgctg agggcagtct gggcgctcga
     1621 atcgatgacc ggcccgacat ccgtttccaa acttgccgga tcgcccatct tcagttccgc
     1681 cattgcgccc ttcagcatct cgatcacagg atcggcaatg tcgtcctgaa ggcacagcac
     1741 gcgaagagcg gaacagcgct gccccgcgga gtcgaaggcg gaggcaagca catcgcccac
     1801 aacctgctct ggaagcgcgg atgagtccac gatcatggcg ttctgtccgc cagtctctgc
     1861 gatcagtgta atattgccgc gctcggccag cgtgccctgg atcgagcgcg ccacctccgt
     1921 ggagcctgtg aagaccacgg cttgcgtgcg cgggtcggca acgagagccg cgccgatgac
     1981 ggagccgcgc ccgggaacaa gctggagaac atcacgggga atgccagcct catgaagcag
     2041 ccggactgcc tccgctgcaa tcaggggcgt ttgctccgcg ggtttggcca gcacgggatt
     2101 tccggcggcg agagcggcca cgatctgccc cgtgaagatc gcaagcggga agttccacgg
     2161 gctgatgcac accgtggggc caagcggcgt atgcggtgca ctcaactcgt ttcgagcctg
     2221 cgccgcataa tagcggcaaa aatcgacggc ctcgcgtact tccgagacgg cgttcggcat
     2281 ggacttgccc gcctcccgga tcagacgggc gctgagttgc tccatcgcgt gttcgagcag
     2341 gtcgccgccg cgttcgagga tggcggcgcg ttcgcccgcg cttgtgttgg cccagcgcgg
     2401 ggccgccgct gcggcggagg ccatcgccgc ttcgacatcc atcttggttg ccgccacagc
     2461 gtggccgacg atgtccgcgt gattggcggg gttgcaaatt gccttccagg gctgcccagt
     2521 tgtggccggt gtcgcgagga tgggctcggc ctcatacgaa gcgccagtgc tatcagcgac
     2581 agtgcgctcc aactcgccgc gcgcacccgc gtcagagaga tcgagcccct ttgaattttt
     2641 gcgttctggc gcgaacaaat cgcctggcag gacaatctgc ggatgcggcg cgccgccctg
     2701 ccgcctagcc tcggcgaccg gatcggcgat caactcctca ataggaattc tttcgtccac
     2761 gagttgatgg acgaaggacg tattggcccc gttctccaaa aggcgccgga cgagataggc
     2821 gagcagcgtc tcatgcgtcc cgaccggcgc gtagatacgg cagggccggt tgaggttttt
     2881 ctcgccgacc acgtgctcat aaagcgtctc gcccatgccg tgcaggcact ggaactcgta
     2941 gtcgccgatc cggaactctg gcccggccat atgatagatt gccgcgagcg tttgggcatt
     3001 gtgcgtcgcg aactggggga aaacggcgtc gcggcgcgcg agcaggcgct ttgcgcaggc
     3061 gagatagcag acgtcggtat aagacttctg cgtataaacc ggatagccgc tcaagccatc
     3121 gacttgtgcg cgcttgatct cgctgtccca atacgccccc ttgacaaggc ggatcatcag
     3181 acggcgcttc gtccgctcgg cgagatcgat aagggtgtcg atcacgaaag gcgcgcgttt
     3241 ttgataggct tgcacgacga aaccgagccc ctgccagcgc ccaagcgcgc tgtcatttgc
     3301 gagcgcttca aaaagatcga gcgacggctc cagtctgtcc gcctcctccg cgtcgatatt
     3361 gaagccgatg ccctgctcga ttgaaagcag ggcgagtttt cggagcctgg gataaagctc
     3421 ggttttgacg cgctccatct gcgtgtagtt gtagcgcgga tgcagcgccg acagctttac
     3481 cgagatgccg gcggattcat gaatgcccgg ctcttcgttc gcgcttccga tagcgttgat
     3541 ggcatgctcg taagcgtcca tgtaattggc ggcgtcttct tcggtgagag cggcctcgcc
     3601 cagcatatcg aaggagtaaa ggaacccgcg ttcctcgaag ggcttggcgt gcccgagcgc
     3661 ttcctcgatc gtcttgccgg tcacgaattg acggccgaga agacgcatcg cgagatccat
     3721 ggcctttctt atgaccggct cgccaccccg agcgagggct cggctgaggg acgcggctat
     3781 gccatgctcg ctgctcgtcg cgacgagctt gccggtgatc agcaatcccc aggtcgaggc
     3841 attcacgaac caggactggc tggcgccgac atgcgcgcgc cagtcgccct gcgagagctt
     3901 gtcgcgaatg agaacgtcgc gcgtctcgtc gtcgggaatg cgtaagagcg actctgcgag
     3961 gcacatcagc gccatgccct cgggtgtcga gagcgagaac tctttcatca gcgcctcgac
     4021 cccgaaggca tgaccgcgtt cggcccgcat tttgttgacg aggccgtggg cgaggttttg
     4081 aaccttggcc gacaaggcag gatccagccg cgcttcctcg accagcgggg ggacgcagac
     4141 cggctcgggc acgcggtacg cagccctgat tgcgccgcgc ttttcttcgc cgcgatcctg
     4201 cgcgttgttt gcttcctgca aaacatccat cggcttcgca tccatggaag aggccctcgc
     4261 gtcgagcatg ccgctgaaag cggtggaaaa gcttaatcac caccttggat atcggtcctc
     4321 gtccgcgcgt cacaagacaa attcccttgc gttcgcggtt taccggttta aaatgactgg
     4381 ctggtcagtc atttcttccg gatgcgatga taccgaagcc tacaacgaag gatcaggccg
     4441 cccccaagag gggcgcccca gacctcagcg agagaaggcg cccgacccag gacgagcgcc
     4501 gcgcggcaat cctcgctgcg gccctcgatg tgttcgctga gaacggtttc gccgctgcga
     4561 gactcgacga tgttgcgcaa aaggcaggtg tcgcgaaggg cactctttat ctgtatttcc
     4621 cggacaagca ggcgctgttc gaagcactgc tccagggtct tgcaggtccc gttctgcagc
     4681 ggttcgaggc gctgtcggcc gatcccgcat tgccggcgag cgcgatactc ggtggtatcc
     4741 tcgccgtgtt tcaaaccgag atcatcggca cgaaccggga gcgtctgatg cgcctcatca
     4801 tcgcggaggg tccccgcttc ccgaagatcg cggaattcta ccatcgcgaa atcatctgca
     4861 agggccgcga aatcatccgc gccatcgccg cccgcgggta tgaacgcggc gagcttccct
     4921 cggatgcgat ggcgaggttt ccgcagctgt tttttgcgcc gctcctgact gcggtgcttt
     4981 ggcggtcgct gttctcgcag ttcgatccgc tcgacgtgaa cgccatgatc gaatgccatc
     5041 gcaagcttat gctcggcctc ggagatgagg agattcccaa atgaaacgtc caccgaaagc
     5101 ggttctcatt gcccttctta ttgctggcgg gctggcaggg gcttggtggt acaagacgcg
     5161 tcacgttggg accggcgagc ttgtcttgca aggcaacgtc gaggttcggc aggtcaatct
     5221 aggtttcaag gttccgggcc gcatcgagac tttgaatgtc gatgaaggcg accgtgtgcg
     5281 caccgggcag gtgctggcgt cactcgataa aatctatttc caggaca
//
LOCUS       DATMGX010000774         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_167383, whole genome shotgun sequence.
ACCESSION   DATMGX010000774 DATMGX010000000
VERSION     DATMGX010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167383"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VNR65_12725"
     CDS             <1..807
                     /locus_tag="VNR65_12725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ19580.1"
                     /translation="QDPSHVEGSLAAILTTFENAFRGYVDANRKLAAQNQALATAKAV
                     TEAANRELEAFSHSVAHDLRSPLRSVDGFCQILDEEYAEKLDDTGRNYLSRVRRAAQH
                     MGTLIDDLLHLARITQGDLARSTVNLSQIAAQIGDSLRDHAPERAVDFTIANDLIVNG
                     DPRLLRVVLENLFSNAWKFTATQPAARIEFGSEPIDGQTALYVRDNGVGFDMAYAGHL
                     FRAFQRLHDVREFPGTGIGLATVQRIINKHGGRIWAKAAPDEGATFFFVL"
     gene            complement(861..1238)
                     /locus_tag="VNR65_12730"
     CDS             complement(861..1238)
                     /locus_tag="VNR65_12730"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWJ19581.1"
                     /translation="MAEAKMEVPQEMREFVAKNIDQAREACNQLMEATSKAQDMMKNL
                     IPANPAVEGLGEVTDRAMRFTRQNLDAGFSLAHELAKAKDLTEMLQIQTRHAQLQMHA
                     CALQAQELGTLVTAAIQKAKPGS"
     gene            1614..2126
                     /locus_tag="VNR65_12735"
     CDS             1614..2126
                     /locus_tag="VNR65_12735"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HWJ19582.1"
                     /translation="MNPIGRAIETVISQSRWLIVPFLVGLVIGLAALAYSFVLKLATF
                     LSSIGKATEDEVIVGILKLVDLSLTANLLLIVIGAGYMNYVSRVDPAEHPNWPPGLIG
                     IGFSDLKQKLLGSIIAIAAVNVLEWFMDIDRSVDNTKLGWVVGILLAFALAMLMLAIA
                     DRVGNSHKNE"
BASE COUNT          468 a          717 c          667 g          543 t
ORIGIN      
        1 caggaccctt ctcatgtcga aggaagcctc gcggctatcc tcaccacgtt cgagaacgct
       61 tttcgcgggt acgtcgatgc gaaccgcaag ctcgccgccc aaaatcaggc gcttgcgaca
      121 gccaaggctg tcaccgaggc agccaatcgc gagttggaag cattcagtca ctccgtcgcc
      181 catgaccttc gctcgccgct ccgaagcgtg gatggattct gtcaaattct ggatgaagag
      241 tatgcggaaa agctggatga cacaggccgc aattatctga gccgggtccg ccgggctgcc
      301 cagcacatgg gcacgctgat cgacgatctc ctgcaccttg cccgcatcac tcaaggcgac
      361 ctcgcgcgct ctacggtcaa tctgagccag atagcggcac agatcgggga cagtctacgc
      421 gatcatgcgc ccgaaagagc cgtcgacttc acgatcgcaa acgatctcat cgtcaatggc
      481 gatccgcggc tgctgcgcgt cgtgttggag aatttattct ccaacgcctg gaagtttacg
      541 gcaacccagc cggcagcgcg catcgagttc gggtccgagc ccatcgatgg gcaaaccgcg
      601 ctttatgtcc gcgataatgg agtcgggttc gatatggcat acgcaggcca tctcttccgc
      661 gcgtttcagc ggcttcatga tgtccgcgag tttcccggca caggcatcgg gcttgccacc
      721 gtgcagcgta tcatcaacaa gcatggcggg cggatatggg cgaaggcggc gccggacgag
      781 ggcgcgacct tcttctttgt tctttagcaa gggacggcgc atccttgcac catcgcctgt
      841 gcttttggcc tgcgaaccgc ttagctgccc ggttttgcct tctgaatagc agcggttacc
      901 agcgtgccaa gctcctgggc ttgaagagcg caagcatgca tttgcaactg ggcatgcctg
      961 gtttgaattt ggagcatctc cgtcaggtcc ttcgccttgg caagctcatg tgccaacgag
     1021 aaaccggcat caaggttttg ccgcgtgaac ctcattgcgc gatccgtgac ctcgcctaat
     1081 ccttccaccg ccggatttgc cggaatcaag tttttcatca tgtcctgcgc tttggacgta
     1141 gcttccataa gctggttgca cgcctcgcga gcctggtcga tgttcttggc tacgaactcg
     1201 cgcatctcct gcggaacttc catcttggcc tcggccatgt tcttcctccc tttgctgcta
     1261 gatctcgcag cattgcccta cggtgtacga ttcaggcgtt gcaagagctc agcgagcctt
     1321 ctgtccccgc tcgcggccca atatgcatca ccgacaaaga atcatactag cgaaacaaag
     1381 ccctttggga ccatgttttt tttgagcgat tgtcttccga gcactcggaa tccatggtgg
     1441 cgtctgaggc gtcggatttt ttggcggccc atccatgaca gacagcggaa caaattaccg
     1501 ctatgatcgg gcttccagtc tggactgctc cggctttcgc tggcaggtta agccacacag
     1561 tactccattt agccgcttcg ccgcatcccg aaccagcccc cgaagagacg ctcatgaatc
     1621 cgataggacg tgcgatcgag acggtcatct cgcaaagccg ctggctcatc gtgccattcc
     1681 tggttgggct ggtgattggg ctggccgcgc ttgcctactc attcgttctg aagctcgcca
     1741 cctttctgag ctcgattggg aaggccacag aagatgaagt gatcgttgga attctgaagc
     1801 tcgtggacct ttccctgacc gccaatctgc ttctgattgt gattggtgcg ggttacatga
     1861 attacgtttc ccgcgtcgat cctgccgagc atcccaactg gccgcccggc cttattggga
     1921 tcggcttttc agacctgaag cagaagctgc tcggctccat tatcgccatt gccgcggtta
     1981 acgtgctcga atggttcatg gacatcgatc ggagcgtcga caataccaag ttgggttggg
     2041 tcgtaggcat tctgctcgcc ttcgctctcg ccatgctgat gctcgccata gcggaccggg
     2101 ttggcaactc acataagaat gaatagcagg ctcggaatgg cctgctgtcc tcgatcacgt
     2161 catcagcgca ggtgcattga aagtcacgcg gcgccgaaga caatagccag ggtattggtg
     2221 cgtccggctg tgaggcaaga gctgctaagg gcggctcgaa gccatcaagg gacgcagctc
     2281 aatcccggcg ctttcgaggg cacggcggac cgcagcggca accggaacag ctcccggcac
     2341 atcgccgtgg atgcacagcg tatcgacttc gagtttgatc ttcgcgcctg ttccc
//
LOCUS       DATMGX010000775         2769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_357689, whole genome shotgun sequence.
ACCESSION   DATMGX010000775 DATMGX010000000
VERSION     DATMGX010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357689"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..596)
                     /locus_tag="VNR65_12740"
     CDS             complement(120..596)
                     /locus_tag="VNR65_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HWJ19583.1"
                     /translation="MNLMKMFAAGTLALMISTSASKAADIVDTAVAAGSFKTLVAAVK
                     AADLVGTLKSPGPFTVFAPTDAAFKKLPKGTLANLLRPENKDKLVKILTYHVVPGKVM
                     AADLAGKTIAVATVEGSRVQVNGTHGVRVNDAHVVKADIVADNGVIHVIDKVLLPN"
     gene            740..973
                     /locus_tag="VNR65_12745"
     CDS             740..973
                     /locus_tag="VNR65_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19584.1"
                     /translation="MIWITAINHRRVSGSTRAKYELHPIKTAQREIEARVWSLIHDHP
                     TLKASLSRASGAYEIAKFTKLEGRPNLDNGMDN"
     gene            1285..1917
                     /gene="folE"
                     /locus_tag="VNR65_12750"
     CDS             1285..1917
                     /gene="folE"
                     /locus_tag="VNR65_12750"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWJ19585.1"
                     /translation="MNELVRLARSSSHAVSSEAMPSQLEIEQSFREVLRWIGEDPDRD
                     GLRETPGRLVRAFQEYFSGYRQDPELILQKTFEETDGYDEMVVLRGVPFESHCEHHVA
                     PIIGRAFVAYVPNSRVVGISKLARVVEAYAKRLQIQERMTAQIANTIEKVLKPQGVAV
                     VIKAAHHCMSSRGVRVHGTDLVTSRMLGCFRDNPLTRQEFMAMVHSGGQE"
     gene            1954..2364
                     /locus_tag="VNR65_12755"
     CDS             1954..2364
                     /locus_tag="VNR65_12755"
                     /inference="COORDINATES: protein motif:HMM:NF021462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HWJ19586.1"
                     /translation="MMTYITGYVSALLTFFFADMAWLGTMAPRFYRPTLGDIAISGVN
                     LAPAMVFYALYPVGLLIFATSPALKSGSITTAVGYGALFGFFTYATYDLTNYATLRNW
                     TLQLTLVDVAWGTILAAITSAVTFWVVSKFVGAG"
BASE COUNT          576 a          743 c          790 g          660 t
ORIGIN      
        1 agatcgaagc cgttggtcaa gaggatatgc cggatgctac gcagaaatag tcgcttttca
       61 gccagtcaaa gcgctttcgt ctacgccccg actggctgcc actttagctc aacaaatcat
      121 taattcggca gcagcacctt atcgatgacg tgaatcacac cgttatctgc gacgatgtcg
      181 gcctttacga catgggcgtc gttcacgcgc acgccatgcg taccgttgac ctggacccgg
      241 ctcccttcga ccgttgctac tgcgattgtc ttgcctgcga ggtccgcagc catgactttg
      301 ccgggcacaa cgtgatatgt gagtatcttg acgagcttgt ccttgttctc tggcctgagc
      361 aggtttgcca acgttccctt gggcagcttc ttgaaggctg catcagtcgg cgcgaagacg
      421 gtaaatggtc ccgggctctt caacgttccg acaaggtccg ccgccttgac ggctgcaacg
      481 agtgtcttga acgaaccggc agcgaccgcg gtatcaacga tatcggcggc ttttgatgcc
      541 gacgttgaga tcattagggc aagcgtaccc gcggcaaaca ttttcatgag attcataaga
      601 ttagatcctt tagagatagt tgttgggaaa cgcccgaact gagggctgtt gagccgaggc
      661 ttcagcagct tccgcgaatg gttctggatc gtgccgcggg agttagaaac ggactcatgg
      721 ggctgttacg gcgctgccag tgatttggat caccgcaatc aatcacagac gcgtgagtgg
      781 gtcgacccga gcgaaatatg aactgcatcc gatcaagacg gcccagcgcg agatcgaagc
      841 aagagtttgg tccttgatcc atgaccatcc aacgctcaag gcttcactgt cgcgcgctag
      901 tggcgcctac gaaatcgcca aattcacgaa attggaaggt cggccgaatt tggacaatgg
      961 aatggacaac tgacgatcgg ctggccaaag ctgcctttga acacgtgatc ctgtcagttc
     1021 aaacgcaaat agagttggaa taagcggttt cgcctacgtt gagcagcgtc gggatgcttc
     1081 gcccctcgcc gtcacagtct agatgtaggt agtctcggtt tcggggtcaa acatcgggtc
     1141 gaattccttg gacaaaaata ttcattccga atggccgggt ccgggtgatc catcagcgtc
     1201 gaaccagcgt taccgaaact gtgtcgcatg aaacaacggg cttggtggcg ctctgccggc
     1261 gtcccccgca attggagctc tgatatgaac gaattagtcc gtttggcccg gtcatccagt
     1321 catgcggtgt cttcggaagc aatgccttcg caactggaga tcgagcagtc gttccgagag
     1381 gtccttcgct ggatcgggga agatcctgat cgcgacggcc ttcgcgaaac ccccggtcgt
     1441 ctggttcgtg cctttcagga gtacttttcc ggctatcgtc aggaccccga gctaatcctt
     1501 cagaagacct tcgaagagac cgacggctat gacgagatgg tcgttctccg cggtgttccg
     1561 ttcgaaagtc attgtgaaca tcacgtcgcc ccgatcatcg gccgtgcttt tgtcgcctat
     1621 gtgccaaata gccgcgtagt gggtatctca aagcttgcgc gggtcgttga ggcctatgcc
     1681 aagcggctgc agatccagga acgcatgacg gcgcagatcg ccaacaccat tgaaaaggtg
     1741 ttgaagcctc aaggtgtcgc cgtggtgatc aaggcggcgc accattgcat gtcaagccgg
     1801 ggcgtccgcg ttcatggaac cgatctggtg acaagccgaa tgcttgggtg tttccgcgac
     1861 aatccgctga cccggcagga gttcatggcg atggtgcatt ccggcgggca agaatgaacg
     1921 ttgccactcg cggcagacat cagtgaggcg ataatgatga cctatattac cggatatgtt
     1981 tccgctctat tgacgttctt ttttgccgac atggcgtggt tagggacgat ggcgccgcgt
     2041 ttctatcggc caacactggg agacatcgcc atatctggcg ttaaccttgc gcctgcgatg
     2101 gtgttttatg cgctctatcc ggtcgggctt ctcatctttg cgaccagtcc ggccctaaag
     2161 agcggatcga tcaccacggc tgttggttat ggcgcgctgt tcggcttttt cacctatgcg
     2221 acttatgatt tgacgaacta cgcgacgctt cgaaattgga ctttgcaact gacgctggtt
     2281 gacgttgcgt ggggcactat tttggcagcc atcacgtcgg ccgtgacctt ttgggtagtc
     2341 agcaagtttg ttggcgccgg ttgatacttg tatttgaact tacatcacga catttcgagt
     2401 cgaagccttc gccataatct cagggggatg gcatgctagc cgatgcgacc agcgcgacgc
     2461 catcggcatt ggtaatcgag gtggatcaac gctttctttc cgacggtctc gaccgtgcat
     2521 cggtgatcgt ttgcggctcg aactcgcata aaaatcaggc acgatctccg cagcggcgac
     2581 gactaatagc gacccgcaca attgagtcga ttgcccatcc ctgacaagcc gaaggcgcct
     2641 cagtggaatc gtgccgggtc tttcgctgga aagggcggcc gaggttctcg gcgttcggca
     2701 tggaacggct gcgatcctgg gtggcacaga aattttcggg ctcttttcta ccccgctatg
     2761 ccgtgtttc
//
LOCUS       DATMGX010000776         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_185724, whole genome shotgun sequence.
ACCESSION   DATMGX010000776 DATMGX010000000
VERSION     DATMGX010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185724"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1941
                     /locus_tag="VNR65_12760"
     CDS             115..1941
                     /locus_tag="VNR65_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HWJ19587.1"
                     /translation="MQRAAALMEVIHERGKKGLPLERLYRHLFNPELYLRAYGKIYRN
                     GGSMTPGVNEETVDGMSLKKIQAIIDALRNERHRWAPARRVYIDKKGASGKRRPLGLP
                     SWSDKLLQEVIRSLLEAYYEPQFSDHSHGFRPGKGCHTALAEVSDSWRGTTWFVEGDI
                     SQCFDSLDHAVMRSILAEDIHDNRFLRLIDGLLQAGYLEEWRYHETLSGAPQGGVLSP
                     LLSNIYLNRLDRYVETNLLPVYNRGARRKPYLPYMQMHKAVWKLEKKGQRERTRQMRR
                     QLQQLPSRDPHDPDFRRLHYVRYADDWLLGFTGTRREAEDIKEKIAEFLGDHLNLELS
                     ERKTLITHARTEAARFLGYEIVVHSNDAKHDRHGHRSINGQIGLKVPMDVVRAKRKPY
                     LRRGKPAAVLARAHDSDFRIVARYQAEFRGIAEYYQLAYNRHRLGLLRYVMERSMTKT
                     LGHKSKISVNKVWNRYRATWPTPAGPRRGLQVTVERGAGRRPLVARWGGVSLARRTTK
                     VVLKDELPTIWRKRPAELIDRLMSGRCELCQAQADVEVHHVRRLQDLQLGNRAEQPDW
                     AQRMAARHRKTLIVCRHCHDGIHHGSPERQGSRNPTLESRVR"
BASE COUNT          495 a          643 c          661 g          361 t
ORIGIN      
        1 cggagtgcca atagtagtcg tgggagtaac gccccaccaa ggagagcggg aaagccgctc
       61 acagggccaa gtggcacagg tgtccacgat tatccggaaa ggcaggtatg cgtaatgcag
      121 agagccgcag ccctgatgga agtcatccac gaacgcggca agaaaggact accactggag
      181 agactgtacc ggcacctgtt caacccggag ttgtatctgc gtgcctacgg gaagatctac
      241 cgcaatggcg gctccatgac gcctggcgtc aatgaggaga cagtggacgg catgagtctg
      301 aagaagattc aggcgatcat cgatgcgcta cggaatgagc gccatcggtg ggccccagcg
      361 cggcgtgtat acatcgacaa gaaaggagca tcagggaaac gccgcccgct gggtctgcct
      421 tcttggtcgg ataaactgtt gcaggaagta atccgctcgc tgttggaagc gtattacgaa
      481 ccacagttct ccgatcactc ccacggtttc aggccgggca aggggtgtca caccgctctt
      541 gcggaagtat ccgactcatg gcgcgggaca acatggttcg tcgagggaga catctcccag
      601 tgtttcgatt cgctggatca cgcagtgatg cgttccatcc tggccgagga cattcacgac
      661 aaccgcttcc tgcgattgat cgatggacta cttcaggccg gatatttgga ggaatggcgc
      721 taccacgaaa cactgagcgg agcgccgcaa gggggcgtgc taagcccctt gctctccaat
      781 atctacctga accggctaga caggtatgtc gagacaaacc ttctccccgt ctacaaccgg
      841 ggtgcccgac gtaagccata tcttccgtac atgcagatgc acaaggcggt ctggaagctg
      901 gagaaaaagg ggcagcgtga gcgaacgcgg cagatgcgtc ggcaactgca acaacttccc
      961 tcacgcgatc cccacgaccc agacttccgg cggctgcatt acgtccgcta cgcggacgac
     1021 tggctgttgg ggttcaccgg cactcgacgg gaagccgagg atatcaagga aaagatcgca
     1081 gagttcctgg gcgatcacct caacttggaa ctctccgaac ggaagacctt gataacccat
     1141 gcgcggaccg aagcggcccg cttcctcggc tacgaaattg tggtccacag caacgacgcc
     1201 aagcacgatc ggcacggtca tcgctccatt aatggacaga tcggactgaa agtaccaatg
     1261 gacgtcgtgc gcgccaagcg caaaccctat ctacgccgcg gtaaaccggc cgccgtgctg
     1321 gcccgcgctc acgattcgga tttccgtatc gtggcgcggt atcaggcgga gttccggggc
     1381 atcgcggagt actaccagtt ggcctacaac cggcaccgtc tcggcctgct caggtatgtc
     1441 atggaacggt cgatgaccaa gacgctgggt cacaagagca agatcagcgt caacaaggtc
     1501 tggaaccgct accgtgccac ctggccgacc ccggcgggtc ctcgcagagg tctgcaggtc
     1561 acggtcgaac ggggcgccgg caggcggcca ctggttgccc gatggggtgg ggtctcgctg
     1621 gcccgcagga ccacaaaggt agttctcaaa gacgaactcc cgacgatctg gaggaaacgc
     1681 ccggcagaac tcatcgaccg gctcatgtcc ggccgctgcg agctctgcca agcgcaagca
     1741 gatgtcgaag tgcaccacgt tcggcgtctg caagacctcc agctcgggaa tcgggccgaa
     1801 cagcccgact gggcacagcg catggcagca cgccatcgca agaccctgat cgtctgccgt
     1861 cattgccacg acggcatcca ccacggaagc ccagaaaggc agggttcgcg gaatccgaca
     1921 ctggagagcc gggtgcggtg ataagtcgca tgcccggttc ggcggggggc cgtcggaaaa
     1981 ggatccgcct catacgcgga tacctcgccg acggcctacc cagcgggcac aggtgatcgg
     2041 atactacgac cgaaaggcat gcggaatgca gaacgccgac acggtactgg gtgtcctccg
     2101 cgaccgtggc agtaaaggcc tgccgtgcaa cgagctgtat cgacagatgt tcaaccgcga
//
LOCUS       DATMGX010000777         4437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_6101,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000777 DATMGX010000000
VERSION     DATMGX010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6101"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /locus_tag="VNR65_12765"
     CDS             <1..1728
                     /locus_tag="VNR65_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWJ19588.1"
                     /translation="RGAARLRAKRAGSQSAHNGRRGRKTKALARRSRGRRHGEIPIGV
                     EKRQAALSGKALAFGITTLSEPVRLKKKSARHDDNAALDENAGSAPPAGKSAEPGGDK
                     PADKDDKSGKKTLQSKAAENAFKLSKESAGGPATLLARLKSLRREGADDDLWSLLRSL
                     NPDTADLADPEKWWDFRRSEVRRALSGDHPATAYAIAKAHGPLDDETRSEAEFMAGWI
                     ALRFMKEPRLAEPHFEAARLIKGYARDEARAAYWLGRTKLELGARKEAEVHFREAASR
                     FYTFYGSLARQALHKAGTCEFRAPPHPTQDSVAAFVNEDAFKAVMIAKQLDLQPLLLN
                     YLLDLTRQLNDPQQMTLALELIERLMPAHVVVRAAKIALLRGFPVDAYAFPTLLPKFD
                     SAGGNGKIELALLNALTRQESDFHTGSVSRVGARGLMQLMPQTAKLVAATYKLKYEPP
                     RLISDPSYNVTLGSAFLAQLLSGYDGSYVLSLAAYNAGPGRVKQWIKDFGDPRSKAAD
                     PIDWVERIPFTETRTYIQRILESTQLYRCRFENNKARFQIVEDLHRGRPGKIPDFDVS
                     GSGELEQTP"
     gene            1725..2687
                     /locus_tag="VNR65_12770"
     CDS             1725..2687
                     /locus_tag="VNR65_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ19589.1"
                     /translation="MTTDRSNDAPGLETEDIFYRSHDDLVLYARKYGSEGAPGRPLVC
                     LAGLTRNARDFHDLAVALSTHPTHPRLVYCLDCRGRGRSEWDKDWRNYSPYVELLDVA
                     AFLTLRNLTNVAFLGTSRGGIITMLLAVTRPSAIGCAILNDIGPVIETAGLARIMGYA
                     GKIPLPRDWDEAINIVRDINIRQFTALDGAGWQKLTHQLFNELDGRPTAAYDPKVGAA
                     LSEVDIAKPVPSMWEHFESLFQVPVMVLRGENSDLLSTSTVQDMQARHPALTSVVIRN
                     EGHAPLLLDRLSQRLIRDFLAKTDKTWVASPPLPASLHNEIQEV"
     gene            complement(2817..3785)
                     /locus_tag="VNR65_12775"
     CDS             complement(2817..3785)
                     /locus_tag="VNR65_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19590.1"
                     /translation="MGIFSSALLCFATFVTTPSEAVPQRPWYLAAFDRVLRVVSNDTD
                     VVASQKDAAKQIEQAYLSRLTKAEEGTITLRGQVPSESDLKILQGVAAATSPGAAVVD
                     RSRVNANVPDRDTWLAAMTFALRQLGKLQSGSAVLRNTAIIIEGVTKADDDFTAMQRK
                     LREEAPKGLYLQVALKPHDVHPFVWIAQLQQGTLNLTGYVPDQQDQDLCSYARALFQN
                     VKVNNGMEVAKGEPQDWLAATKLSLEMLSLLFSGNVAISDNVIKLDGIYSSPAMVELF
                     HAYSKRLPPGFKLETRITEPVAKAPAPAEDVSLAVHNSQPQTSMNT"
     gene            complement(4109..>4437)
                     /locus_tag="VNR65_12780"
     CDS             complement(4109..>4437)
                     /locus_tag="VNR65_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19591.1"
                     /translation="VLLGITKASLQTRSFISAASFQETTRVLTEAALNGKVDTLDGLK
                     ENVIVGRLIPAGTGGMISQLRRVASHRDDLILQERSKAGAKALLPAGEGESPAPDLEP
                     QVQAAE"
BASE COUNT          955 a         1275 c         1261 g          946 t
ORIGIN      
        1 aggggggccg ccaggcttcg cgctaagaga gccggcagcc aaagcgctca taatggccgg
       61 cgtggccgga agacgaaggc cctcgcacga cgcagcaggg gacggcgtca cggcgaaatc
      121 ccaatcggcg tagagaaaag acaagccgca ttaagtggaa aagcgcttgc gttcgggatc
      181 acaacccttt ctgagccggt tcgtctcaag aagaaaagcg cgaggcacga tgacaatgcg
      241 gcgctggatg agaacgcggg aagtgccccc ccggccggca aatccgcgga accgggcggc
      301 gacaagcctg cggacaagga cgataaatct ggcaagaaga cgctccagtc gaaagcggcg
      361 gagaatgcct tcaagctttc gaaagaaagt gccggagggc ccgcgacgct tctcgcgcgg
      421 ctgaaatcgt tgcggcgcga gggagcggac gatgacctct ggtcgctctt gcgctcgctc
      481 aatcccgaca ccgctgatct tgccgatccc gagaagtggt gggatttccg gcgaagcgaa
      541 gtaaggcgcg cgctaagtgg cgaccatccg gcaaccgcgt acgcaatcgc caaggcgcac
      601 ggcccgcttg acgatgaaac ccgctccgaa gccgagttta tggcgggctg gatcgcgctt
      661 cggttcatga aggagccgcg cctcgccgag ccgcatttcg aggcggcacg cttgataaag
      721 ggctatgctc gagatgaggc gcgggcggca tactggctcg gtcgcaccaa gctcgaattg
      781 ggcgcgcgaa aggaagcgga ggtccacttt cgcgaggcgg cgagccgctt ttatacgttt
      841 tatggttctc ttgcccggca agctctccac aaggcgggca cctgcgagtt ccgtgcaccg
      901 cctcatccaa cacaagactc tgttgccgcc ttcgtgaacg aggacgcatt caaggctgtc
      961 atgatcgcga agcagctcga tctccagccg cttctgctga actacttgtt ggacctgacg
     1021 cggcagctca acgatcctca gcagatgaca ctggcgcttg aactgataga gcgcctgatg
     1081 cccgcccacg ttgtggttcg tgcggcaaaa attgcgttgt tgcgtggatt ccctgtggat
     1141 gcctatgcct tcccgacact cttgccgaaa ttcgattccg ctggcggaaa cgggaagatc
     1201 gaactcgcgc tcttgaatgc cttgacccgt caggaaagcg atttccacac gggctccgtc
     1261 agccgggttg gcgcgcgcgg gctcatgcaa cttatgccgc agacggcaaa gctcgtcgcg
     1321 gcgacctaca aattgaagta tgagccgccg cggcttatct cagacccgtc ctataacgtc
     1381 accctgggct cggcttttct ggctcagctg ctttccggtt acgatgggtc ctacgttctt
     1441 tctttggctg cgtacaatgc agggccaggc cgggtgaagc agtggatcaa ggattttggt
     1501 gatccgcgga gcaaagccgc ggacccgatc gattgggtcg agcgcattcc atttacagag
     1561 acgcgcactt acatccagcg cattctcgaa agcacgcagc tttaccgttg ccggtttgaa
     1621 aacaacaagg caaggtttca gatcgtagaa gatttgcatc gcgggcgtcc cgggaaaatc
     1681 cctgatttcg atgtgagcgg aagcggcgaa ttggaacaga ccccatgact acagatagat
     1741 cgaacgacgc tccggggttg gaaacagaag acatctttta tagaagccac gacgatctgg
     1801 ttctctacgc tcgtaaatat ggtagcgaag gagcccctgg ccggccgctt gtttgcctcg
     1861 caggtctgac ccgcaatgcc cgggattttc acgatctcgc tgtcgccctc tcaacccatc
     1921 cgactcaccc ccggcttgtc tactgcctcg attgcagagg ccgcgggcgc tccgaatggg
     1981 acaaggattg gcgcaattat tccccttatg ttgaactgct tgatgtcgcc gccttcctta
     2041 cgctacgcaa cctgacgaac gtcgccttcc ttggaacgtc acgcgggggc atcatcacca
     2101 tgcttctcgc ggtgacgcgg ccttcggcca ttggatgcgc gatcctgaac gacatcggcc
     2161 cagtcatcga gaccgcaggc ctcgcccgca tcatgggcta cgcggggaaa attcctctgc
     2221 cccgggactg ggatgaggcg ataaacattg ttcgcgacat caacatcagg cagttcacgg
     2281 ctctggacgg tgcagggtgg cagaagctga cgcaccaact tttcaatgaa ctggatggac
     2341 gccccaccgc ggcttacgat ccgaaagtgg gagcagcctt atccgaagtg gatatcgcga
     2401 aaccagtccc gtcgatgtgg gagcacttcg aatcgctttt tcaggtgccg gtgatggtgc
     2461 tgagaggaga aaactcggat cttctatcga cttcaaccgt ccaagacatg caagcccgtc
     2521 accctgcttt gacatcggtt gtcattcgca atgagggcca cgcacctctc ttgctggatc
     2581 gcctcagcca gcgcctgatc cgcgatttcc tagccaagac ggacaagacc tgggtggcgt
     2641 cacccccgct gcctgcttct ctgcacaacg agatccagga agtttgaagg caccgctaga
     2701 ccaacccgct cttaatccgg aatcgattaa gagcagaaaa ttgaccgatc ctaaagtata
     2761 gagcagctta gcttatccgc gtccaagtgg acgtgggttg ctaggcttcc gagttttcag
     2821 gtgttcattg atgtctgagg ctgagaattg tgaactgcca gactgacgtc ttctgcagga
     2881 gcgggcgcct ttgcgactgg ctcggtaatc ctggtttcca gcttgaagcc gggcggcaat
     2941 ctcttgctgt aggcgtggaa caattccacc atcgctggcg atgaatagat cccatcaagt
     3001 ttgatgacat tatcgctgat tgcgacattg ccggagaaca gcaaagacaa catctcaagc
     3061 gacaacttcg tggcggcgag ccaatcctgc ggctcccctt tagccacctc catcccgtta
     3121 ttaactttca cgttttggaa aagcgcacga gcataggagc acaaatcctg atcctgctga
     3181 tctggcacat agccagtcaa attcagagtg ccttgctgca gttgcgcgat ccagacaaag
     3241 ggatggacat catgtggctt gagggcgact tgtaaatata ggcctttggg cgcctcttcg
     3301 cgcagcttcc tctgcatcgc tgtaaaatcg tcatcggcct tagtcacacc ttcgataata
     3361 atcgcggtgt tacgcagcac tgccgaaccc gactgaagtt tgccaagctg gcgaagcgcg
     3421 aaggtcatcg cagccaacca ggtatcgcga tctggtacgt ttgcgtttac gcgactccta
     3481 tcgacgaccg cggccccagg cgaggttgcc gccgcaacgc cctgcaaaat cttgagatca
     3541 ctttcggacg gtacctgtcc cctgagcgtg atcgttcctt cttctgcttt tgtcaggcga
     3601 ctgaggtaag cctgttcgat ctgcttagcc gcgtctttct gcgatgctac aacatctgtg
     3661 tcgttgctga cgacgcgaag aacacgatca aaggccgcga gataccaggg gcgctgagga
     3721 acagcttccg atggtgttgt aacgaacgtc gcaaagcata acaaagcgga tgaaaagata
     3781 cccaattctg agtttcctaa cctcattgcg cgagttctcc accgcattca cattcagggt
     3841 cctgcatctg ttgaggccgg tttttcacca aggcgtttgt gtcacggttt gtcatccttc
     3901 acccgccgaa acgaataggc cgccctctcg gcccctttat cggccgatgc aaagcagcac
     3961 catttgtcgg taaatgagac tttaacggag agtttccgat aatccaagcg cgtcttggcg
     4021 ctttttcgcc tctttttttg tttgcagcgt gacatgaatc aaagaagccc ggcagatacc
     4081 cgggcttctt aagtgtttga gatgcaatct attcggcggc ctgcacctga ggctccaggt
     4141 caggggcagg tgattcgccc tcgcccgcag gcagaagagc tttcgcccca gccttgctac
     4201 gctcctggag aatcagatcg tcgcgatgcg acgcaacgcg ccggagttgg ctgatcatgc
     4261 cgcccgtgcc ggctgggatg aggcgaccca caatgacgtt ttccttgagg ccgtcgagcg
     4321 tgtcgacctt accgttgagc gccgcttcgg taaggacgcg cgttgtctcc tgaaaggaag
     4381 ctgccgagat gaacgaccgc gtttggaggc ttgctttggt gataccgaga agaaccg
//
LOCUS       DATMGX010000778         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_262232, whole genome shotgun sequence.
ACCESSION   DATMGX010000778 DATMGX010000000
VERSION     DATMGX010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262232"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VNR65_12785"
     CDS             complement(<1..888)
                     /locus_tag="VNR65_12785"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HWJ19592.1"
                     /translation="MEPFVLGWREWVALPQLGLPALKAKIDTGAKTSALHAFSIETFG
                     PIDAPRVRFGVHPVDYHNEIEIWCTAKIVDRRHVRSSNGQAENRYIIHTPIRIGDREW
                     PVEISLSNRYTMAHRMLLGRTALEAQAASILPWSEYRQEKLSYDAYADQERVAPVKRS
                     LRIALLTMEPGNFSNKRFIEEAEKRDHVIEPINTLRCYMNIKSIASAVHYEGKTLPDF
                     DYVIPRIGASITAYGTAVVRQFEMTGAHVLNSAQSIVQSRDKLFAHQLMAMHKLPMPD
                     TAFASSHHDKDDLIKMVGGT"
     gene            1454..2173
                     /locus_tag="VNR65_12790"
     CDS             1454..2173
                     /locus_tag="VNR65_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ19593.1"
                     /translation="METTLHILVVDDHKDIRDLLAKFLVKHGMRVTTAADATEARRHL
                     KAGAFDLIVLDIMMPGEDGISLCRSLREGAAPPIIFLTAVAEDTDRIIGLELGADDYV
                     TKPFNPRELLARIRAVIRRARSVPQSRDAPAGERLKFGQFIFDADRRELINGQDIATP
                     LSYGEYLLLSAFLHRPNIVLNRDQLLDITRGRAANLFDRSIDNQVSRLRRKIEDDPKD
                     PKIIQTIWGGGYKFVGVVERL"
BASE COUNT          492 a          632 c          627 g          565 t
ORIGIN      
        1 tgtgccgcca accattttta tgaggtcatc cttatcgtga tgcgagcttg caaacgctgt
       61 atccggcatc ggcagcttgt gcatggccat gagctgatgg gcgaacagct tgtcccggct
      121 ttgcacgatg gattgagctg aatttagcac atgcgcaccg gtcatctcga attgccgcac
      181 cacggcagtt ccgtaggccg tgatcgatgc gccgatccgc gggatcacat agtcgaaatc
      241 gggtagcgtc tttccttcgt aatgaacagc gctcgcgatt gatttgatgt tcatatagca
      301 gcgaagcgtg ttgatcggct cgatcacgtg gtcccgtttc tcggcttcct caatgaagcg
      361 tttgttcgag aaatttccgg gctccatcgt gagaagggcg attctgagcg agcgcttcac
      421 aggcgcaaca cgctcctggt ccgcgtaagc gtcgtagctc aacttttcct ggcggtactc
      481 ggaccaaggc agaatgctgg cagcttgggc ttcgagagct gtgcggccca gcagcatccg
      541 gtgcgccatc gtatagcggt tggagaggga tatttctacg ggccattccc ggtcgccgat
      601 gcgaatgggc gtgtgaatga tgtagcgatt ctctgcctgc ccgttcgagc ttcgaacatg
      661 gcgccggtcc acaatcttcg cggtgcacca gatctcgatt tcgttgtggt aatcgacggg
      721 gtgaacgccg aaccttacgc gcggcgcatc tatcggccca aacgtctcga tcgagaacgc
      781 atgcagcgcg gaggtcttcg cgccggtatc gattttcgct ttcaaagcgg gtaagccaag
      841 ctgcggaagc gcgacccact cgcgccagcc caaaacaaat ggctccatca aaccttcccc
      901 ggttttctca atccaatccg atacgaacga tgcttttttg ccgcgggctt acgcagggtc
      961 ttatgaagcc tctgtcttaa tacgtcaaag ctaacgcaat gccggaagct cttgttccgc
     1021 tccttcaatt cgcggcagac gctagaggag aaacaattcc attttatgac aagtgatttg
     1081 aattcatttt tgtcgaagct ctctagttac tccgattggg caaccataga tgcatttttg
     1141 cctttcgaaa gcaatacaag tttacgaatt ttaagctggt atggatgcac cgcaacttta
     1201 tattgttcag atcgtcgacc atgcggttga tcggtcctcg ctgccgtcat cacactcgct
     1261 tatatgccgc tgaatcgata tctcgcgtct aacttagact tgacccggtt tgcaggcgga
     1321 cagataggtg gtaacccttt ttaagcatca aagtgcgtcg cattgcgtaa gacagcctct
     1381 ttgccacagg tgcttacaca tttgatgctg cattgttatg cgggcgctct aaacatgggc
     1441 cagaggagag gccatggaga ctacgctaca cattcttgtt gtcgatgatc ataaagacat
     1501 tcgtgatctg ctcgcgaagt ttcttgtgaa gcacggcatg cgggtgacga cagcggctga
     1561 cgctacggaa gcgcggcggc atctcaaagc aggcgcgttc gatcttatcg ttctcgatat
     1621 catgatgccc ggcgaggacg ggatctcgct ttgccgctct ttacgcgaag gcgcagcgcc
     1681 tccgatcatt tttcttacgg cggttgcgga ggatacggat cgcatcatcg gtctggagtt
     1741 gggggccgat gactacgtca cgaagccgtt caacccaagg gaacttttgg cgcgcatccg
     1801 cgccgtcata cgccgcgcac gaagcgtgcc ccaaagccga gacgctcctg ccggcgagag
     1861 gctgaaattc ggacagttca ttttcgatgc ggatcgccgc gagttgataa acggtcagga
     1921 cattgcgacg ccgctctcct atggcgaata tctccttctc tcggccttcc ttcatcgccc
     1981 caacattgtg ctgaaccgcg accaactcct cgatatcacg cgggggcgcg ccgcaaatct
     2041 gttcgaccgg agcatcgaca accaggtcag ccggcttcgc cgaaaaatcg aggatgatcc
     2101 aaaagacccg aaaatcatcc agacgatctg gggcggcggc tacaagtttg ttggtgtggt
     2161 tgagcgccta tgacgcgctt tttgccacga agcctgaagg gccagctcat cctcctcatc
     2221 ctggttgggc ttttgctttc gcaagcggcc ggcttggtat tcatcctcaa cgatcagaag
     2281 tccaggataa aaagcgagtg gttgcacaac attttt
//
LOCUS       DATMGX010000779         2014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_194978, whole genome shotgun sequence.
ACCESSION   DATMGX010000779 DATMGX010000000
VERSION     DATMGX010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194978"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..480)
                     /locus_tag="VNR65_12795"
     CDS             complement(211..480)
                     /locus_tag="VNR65_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19594.1"
                     /translation="MPRTPDIPRDFGSAQPLTRFFLRLVIFSIFAAFGSQAFGKTLEG
                     LLVWAVFYCIFAAAVRREQPFGPALTHFDEAAAYAVIAGLAAWTS"
     gene            complement(501..971)
                     /locus_tag="VNR65_12800"
     CDS             complement(501..971)
                     /locus_tag="VNR65_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19595.1"
                     /translation="MLSDPKHEDRLDRLTEQLRLAPALTPNLIGAVIADACTRLPVLH
                     RAGMAARIDQLIEAGAWSDAALALIELELPAWKLRRLIYEDGEWFCSLSKQPNLPVAF
                     DDTADARHEVLPLAILSAFLEARRATSAVRETSSPTVPQVQPTSGIVICCDNFA"
     gene            1614..1703
                     /locus_tag="VNR65_12805"
     CDS             1614..1703
                     /locus_tag="VNR65_12805"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HWJ19596.1"
                     /translation="MIFDYSLAALVTAGLLFYLTYALLRPERF"
     gene            1714..>2014
                     /locus_tag="VNR65_12810"
     CDS             1714..>2014
                     /locus_tag="VNR65_12810"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWJ19597.1"
                     /translation="MTIIGWIQIILFCAIIVALTPVLGAYMTRVFNGERTFLSPVLRP
                     IEAGMYWAGSIDEKREQHWLTYTVAMLLFHAGGFLIIYAILRLQDVLPFQAQLNPA"
BASE COUNT          416 a          566 c          594 g          438 t
ORIGIN      
        1 aggttttcga gcacaagggc agtcatcgaa gtctctcctt gttgttcttg cggaagctct
       61 tttgcctccg tgccgctaat gtgcccacga ccgcatatga attcgagacg gaacggcgct
      121 gcttctcata aaggcgatat aaagagaccc tgcactgcac cggttaccgc cagaatttga
      181 ttggggcttg gcattgcttg ttccgccgaa ctacgaagtc cacgcagcca ggccggcaat
      241 tacggcatat gccgctgcct cgtcgaaatg agttagtgcc gggccgaacg gctgctcgcg
      301 tcggaccgcg gcggcgaaaa tgcagtagaa cacggcccac acaagcaggc cctcaagggt
      361 cttgccgaac gcttggctgc cgaacgccgc gaaaatgcta aagatgacca atcgcagaaa
      421 aaagcgggtc agcggttggg ctgacccgaa gtctcgggga atgtccggag tgcgcggcat
      481 tttaactcgg cagaggccgc tcaggcgaag ttgtcacaac agatcacaat gcctgatgtc
      541 ggttggactt gcggcactgt tggcgaactg gtttcgcgca cggcgctagt cgcgcggcgc
      601 gcttcaagaa atgcgctcaa gattgccaga ggcaatactt cgtgcctcgc gtctgcagtg
      661 tcatcgaacg ccacaggcag atttggctgc ttggagaggg agcagaacca ttcaccgtcc
      721 tcatagatga ggcgacgaag cttccatgct ggcagttcga gctcgatcag cgcaagcgcc
      781 gcgtcactcc aagcgccggc ctcgatcaac tggtcgatcc gagcagccat tccagccctg
      841 tgcagcaccg gaaggcgtgt gcaggcgtcc gcgatgacag cgccaatgag attgggggtg
      901 agcgccggcg caagccgaag ctgctcggtc aaccgatcga ggcggtcctc gtgttttgga
      961 tcggacaaca tgacaccatt tccaccggaa cagcggtctt atgttgtgcg accgcacccg
     1021 taagatgggg ccaaaggtca taaggaagcg agatggaccg aaacgccctg atatatggat
     1081 gtcataggaa tcggaatggc ctttcataaa gatccaggcg cgcgagctct gcaggattgc
     1141 gcttctgcaa gcgcggccac acgaagctcc gatgcactgg acccggtgtc gcgttccgca
     1201 tccgagtgat ggtgttgcgg ccacaggctt tgctgtctgc aaggaatggc aaactatcgt
     1261 gttatttcaa atggttaatg tcgaaaatta agcgcgtcat ggggaggtca tgacgctgca
     1321 tctttatgag attcctataa cgtcggcctt gtttccatct agaaatttta tggcgatgag
     1381 tgccaagtaa gcgccgctcg cacggggcga gaaatgcttc gggcgggaaa tacttcgcgc
     1441 ttttgccgct tgccaatgcg catcggtaaa ggcgccatct agtgcccacc agggactgcg
     1501 tgtcggaagg agcatccaaa tgttggacct gatcttgctg gccctcgggc tcggcttttt
     1561 cgcgctgtcg gtgggctacg cctacgcttg cgacaggctt tgaggaggct gccatgattt
     1621 tcgattattc gctcgccgcc ctcgtgaccg cggggctgct gttctacctc acctacgctc
     1681 tgctgcgtcc cgagcgattc tgagaggcgt gccatgacca taatcggctg gattcagatc
     1741 atcctttttt gcgcgatcat cgtcgcgctg acgccggtgc tcggcgccta catgacgcgc
     1801 gtcttcaacg gcgagcgcac attcttgtca ccggtgttgc ggcccatcga ggccggcatg
     1861 tactgggccg gtagtattga cgaaaaacgt gaacagcatt ggctgaccta tacagtcgcc
     1921 atgctgctgt ttcacgccgg cggtttcctc atcatttatg caatcctgcg tttgcaggac
     1981 gtgctgccgt tccaggccca gctcaaccca gccg
//
LOCUS       DATMGX010000780         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_394696, whole genome shotgun sequence.
ACCESSION   DATMGX010000780 DATMGX010000000
VERSION     DATMGX010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394696"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1348
                     /locus_tag="VNR65_12815"
     CDS             308..1348
                     /locus_tag="VNR65_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWJ19598.1"
                     /translation="MGQIKKPGQKPEVVTLKAVAQHIGLTPGTVSAVLNDSPSARSIP
                     QETKNRIHAAAKELNYRPNFFARTLRNKRTYTIGVIAEEIGDSYSSPIISGIEQYLRK
                     RDYFFLTVVHRHDPVLLNRYSQLLSERGVEGIITVDTTVQEAPALPTVAIAGHKKLKG
                     VTNITLDHVRAARLALNHLKEMSHERVAFMKGNPVSSDAKDRWDAICQVAAEIGMKID
                     PELTVQIDTDDPTPMLGYPFTKQLLARKKPFTALFAYNDISAIGAIRAILEQGLRVPH
                     DISVMGFDDIPGAAFHTPSLTTVQQPLNRMGEVAAQVLLERIEGKNEDPSDIAIEPEL
                     VVRESTAKAPGA"
     gene            1393..>2069
                     /locus_tag="VNR65_12820"
     CDS             1393..>2069
                     /locus_tag="VNR65_12820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ19599.1"
                     /translation="MPPSDSPNASTSAKALTFAAYASFVPIGIATVLLGPMLPTLSAR
                     WSLNYSQAGALFTAQYLASTLAVALSGVLVSRWGFRFAIKTGLLLMSAGVGLLLTGPK
                     VLGIFCIGAYGGGLGLAVPAANLLVAEVNPGRRSATLNLLNFCWSAGAVACPFLVAAA
                     VKVHQVPLFLGAVAGFALLMAVGIAAMPASIVEPSAKRDVTQKTGLAIPWGHGSLLML
                     AALFFLYV"
BASE COUNT          407 a          553 c          650 g          459 t
ORIGIN      
        1 gatcgttggc ggagccatta ttccggtggc gcaagggtgg attgccgacc atatcgggat
       61 tcaccacgcg ttcttcctgc cagtgatttg ttatctgtac attttgttct tcgctctgag
      121 cggatcgaag ccgaacagcc agcgttatgc caaggcctga ggtccgctgg caagagagtg
      181 tataggttct tcgcttcgct cagaatgaca acggtgtgcc gaatcacaga cactcgtcag
      241 gcatttggta acatggtttg cgacgtaatg tttcgggaaa tacaggatta aaacgattta
      301 agcgatcatg gggcaaatta agaagcctgg ccaaaaacca gaggtcgtta ccctcaaggc
      361 cgtcgcccaa catattggac tgactccagg gacggtatca gcggtcctga acgattcgcc
      421 ttcggcccgg tcgattccac aggagaccaa gaaccggatc cacgcggccg ccaaggaact
      481 caactaccgg cccaattttt ttgcccgcac gctgcgcaac aagcgtacct acaccatcgg
      541 agtaattgcg gaagagattg gcgactccta tagctcgccg attatcagcg gcattgagca
      601 gtatttgagg aagcgcgatt atttcttcct gacggtggtg caccgacacg atcccgtgct
      661 gctgaatcga tattcgcaac tgctatcgga gcggggagtc gagggcatca ttacggtgga
      721 tacgaccgta caggaagcgc ctgcgctgcc tacggttgcc attgccggcc acaagaagtt
      781 gaagggcgtc accaacatta ccctcgatca cgtgcgagcc gcgaggctgg cgctgaacca
      841 tctcaaggaa atgtcccacg agcgagtcgc cttcatgaag ggcaatcctg tgagttcgga
      901 tgcgaaggac cgctgggacg cgatctgcca ggtggcggcg gagatcggga tgaagatcga
      961 tcccgagctg acggtgcaga tcgataccga cgatccgact ccgatgttgg gctatccgtt
     1021 taccaagcag ctgctggcgc gcaagaagcc gtttacggcg ctgtttgcct acaacgatat
     1081 ttcggcgatc ggcgcgattc gggcgatcct ggaacaagga ctgcgggttc cccatgatat
     1141 ctcagtgatg ggcttcgacg acattccggg ggccgcgttt catacgccca gcctgaccac
     1201 ggtgcaacag ccgttgaacc gcatgggaga ggtggcggcg caggtcctgt tggaacgaat
     1261 tgagggcaag aacgaagacc cttcggatat tgcgattgag ccagagctag tcgtgcggga
     1321 atcgacggcg aaggctccgg gagcttaggg tcacggttcc gatatagtag cggcagagcg
     1381 tcctcgccat tcatgccccc ctctgactca cccaacgcct caacctccgc caaggcgctt
     1441 acgttcgccg cctacgcgtc gtttgtgccg atcgggattg cgaccgtact tctggggccg
     1501 atgctgccga ctttgtcggc gcgatggtca ttgaattatt cccaagcggg agccctgttt
     1561 acggcgcagt atctggcttc gaccttggct gtcgcccttt cgggcgtgct ggtttcgcgc
     1621 tggggattcc gctttgcaat caagacggga cttctgttga tgagtgcagg agtgggtctg
     1681 ctgctgaccg gtcccaaggt gctggggatt ttttgcatcg gcgcgtacgg aggaggactg
     1741 gggctggcgg tgccggcggc gaacctgctg gtggcggaag tgaatcccgg gcgacggagc
     1801 gcgacgctga atctgttgaa tttctgttgg agcgcgggtg ctgtggcgtg tccgtttctg
     1861 gtggcggcgg cggtgaaggt ccaccaagtt cctctcttcc tcggagcggt agcagggttc
     1921 gcgctgctga tggcggttgg gatcgcggcc atgccggcgt ccattgttga gccgtcggcg
     1981 aaaagggatg tgacccagaa gacgggcttg gcgattccat gggggcacgg ctctttgctg
     2041 atgttggcgg cactcttctt tctttacgt
//
LOCUS       DATMGX010000781         2260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_202511, whole genome shotgun sequence.
ACCESSION   DATMGX010000781 DATMGX010000000
VERSION     DATMGX010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202511"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            711..>2260
                     /locus_tag="VNR65_12825"
     CDS             711..>2260
                     /locus_tag="VNR65_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWJ19600.1"
                     /translation="MIFRQLGKKLFARLFGDGDFRVFISIFREYFPAYKYQYGLIFLL
                     IGVASVATAAAAWLVRDVVNGLFLTKEGSMLIPIFLLVLFAFLLKGLSTYWQSVLSAR
                     IANDMVAKTQERMISHILMQRAEFFDRFSSDDLIMRISMGAGSFGTILNRVVLAGARD
                     LATVLSLAFVMVRQDLILTLICLIAVPPIFLCISLLLKRIKSLMKQEMQSIAELNKHV
                     REAVQGFKVIKAYNLEPVIKNEVDSVIESIKDRSNKVSALENAPVPIIDTLGGVAVGL
                     TILYAGYRTIYGTYDPGTFMSFITAMLLAMDPARRVSQLRVALRSSLVGVGMVHDMLE
                     DNAPEIEAPTRRGPGDVVPALLRPKGGLGVVMEDVTFAYSPGAANVLDAFTLDVKPGD
                     MVALIGPSGAGKSTVFSLLLRFHRHQAGRILVGGRDIEQTPVTEIRDLIAYVGQANFI
                     FSGSIRDNLTLRRSGVSDDAVIAACKTVGLHDHIMKLPGGYDTFVGELGSLISGGQAQ
                     RLNIARAI"
BASE COUNT          448 a          678 c          634 g          500 t
ORIGIN      
        1 caaaaccggc agcagcgcgc gccaccatgg acaaaggctc ggtcgggcat ttcgatcaat
       61 tgtgcctttt tagcttccca gtccatgggc gctgaacacg ggcgaggttc cgagcaaagt
      121 ggagtcacct gattctcttt acattcgaga acgggatacc atcttgcagg caggaacaac
      181 gaacgaaagg aggtgatccg atgtctagtg ggatatctgc gcttaagccg cggttactgc
      241 gtgtcacgga aacgttggag cagcctctgg cgtcagccta gcactcaagg ctaaagcccc
      301 ttgccggttg gcagggcaaa gccggctgtg gcaaacagcc agtctcacgg gccgcggaaa
      361 ctccgcggcc tttttgctgt cctggatgag cgcggcacgt ttgcgcccat gacgattcgg
      421 aacattatcc ccaaaagaga agcaacggct ggaggccact cagcgcccgt tacccggcac
      481 cttatcgagc cgtacctgac ggtcccactc atgcgccgca ataacatctc attgatccta
      541 atatggttcc agatcaatgg atgctgccgt catgcaaact gctttctgta tccgccatgc
      601 tgtcgatggc gattgagcct tttgcgcaac aggtgctcct cccccggcct tgccccgccg
      661 ccgggcgcgg tgtatcgctg gcgtcgagct tagcagaggg cgggtaatcc atgattttcc
      721 ggcaactcgg gaaaaaactg ttcgcgcgcc ttttcggcga tggcgacttc cgcgtcttca
      781 tctcgatctt ccgcgagtat ttcccggctt ataaatacca atacgggctg atctttctct
      841 tgatcggcgt ggcatcggtg gctacagccg cggcggcttg gctggtccgg gacgtcgtca
      901 acggcctttt cctgacgaag gaagggtcga tgctgattcc catttttctg ctcgtcctct
      961 ttgcgttcct gctgaagggc ctctcgacct attggcaatc cgtgctctcg gcacgcatcg
     1021 caaacgacat ggtcgccaag acgcaagaac ggatgatcag ccatattctt atgcagcgag
     1081 ccgagttttt cgatcgcttc tcgtcggatg acctgatcat gcgcatcagc atgggcgcgg
     1141 gctcattcgg cacaatcctc aaccgcgttg ttctcgcagg cgcaagggat ctggcgacgg
     1201 tcctctcgct cgcattcgtg atggtgcggc aagacctcat cctcacgctt atatgcctga
     1261 tcgcggtccc tccgatcttc ttatgcatct cactgcttct gaaacgcatc aaaagcttga
     1321 tgaaacagga gatgcaatcc attgccgagc tgaacaagca cgtccgcgaa gccgttcagg
     1381 ggttcaaggt catcaaagct tacaacctcg agccggtgat caagaacgag gtcgactccg
     1441 tcatcgaaag tatcaaagac cggtccaaca aggtgtcggc gctcgagaac gcgccggttc
     1501 ccatcatcga cactttgggc ggcgttgccg tcgggctgac aattctttac gccggatacc
     1561 ggaccatata cggcacctat gaccctggca cgttcatgtc gttcatcacg gcgatgctgc
     1621 ttgcgatgga tccggcccgc cgcgtgagcc agctcagagt tgccctcagg tcgtcattgg
     1681 tcggcgtggg catggtgcac gacatgcttg aagacaatgc ccccgaaatc gaggccccca
     1741 caagacgtgg ccccggagac gtcgttcccg ctctgcttcg cccgaaggga ggcttaggcg
     1801 tcgtcatgga ggatgtcacc ttcgcatatt caccaggggc ggcgaacgtg ctcgacgctt
     1861 tcacgctcga cgtgaagccc ggagacatgg tggcgctgat cgggccatca ggcgcaggaa
     1921 agtcgaccgt gttttcgctt ctgttgcgct tccaccggca tcaggcggga cgcatccttg
     1981 ttggcgggcg cgacattgag caaacgcctg taaccgagat ccgcgatctc atcgcctatg
     2041 tcgggcaagc caatttcatc ttcagcggtt cgatccgcga taatcttacc cttcgccgca
     2101 gcggcgtttc agacgacgcc gtcattgcgg cctgtaagac cgtcgggctg cacgatcaca
     2161 tcatgaagct gcctggaggc tacgacacct ttgtgggcga actcggctcg ctcatttctg
     2221 gcgggcaggc tcagcggctt aacatcgcgc gcgccatcat
//
LOCUS       DATMGX010000782         2663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_8239,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000782 DATMGX010000000
VERSION     DATMGX010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8239"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /gene="istA"
                     /locus_tag="VNR65_12830"
     CDS             <1..1424
                     /gene="istA"
                     /locus_tag="VNR65_12830"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HWJ19601.1"
                     /translation="YYRRRNRMLSQAQRTTILELNTQQVSKREIARVLGISRVAVRKV
                     LRSNSTALPELHRTEKAEPYRQQILELFDQCKGNLVRVHEELVAAGAELSYTALTAFC
                     RRHGIGYAPPTPSGLYDFSPGEEIQHDTSPHELELGGKKRKVQTASAVLCYSRMLFFQ
                     CYPNFRRFDCKVFLTEALRFMGGATTRVMIDNTHVVVLRGSGRDMVPVPEMAAFGERF
                     GFQFVAHAIGNANRSGRVERPFHFIENNFLAGRTFRSWEDLNQRAREWCDKVNSTYKK
                     HIRAIPRELFAVERLHLKPLPAWIPEVYRLQQRLVDIEGYVALNSNRYSVPVEWIGRR
                     VEVRETRDKIEIQLDARRLVTHRRIAEAEHQRVMLAEHRPPRGHRDARPDPHPEERAI
                     VTAAPELADYVAGLKQRSRKVVTLALRQLLRLVREYPREPLLGAVREAGRYGLYDLDR
                     LEHMILRRVTREYFLLDGEADDD"
     gene            1417..2142
                     /gene="istB"
                     /locus_tag="VNR65_12835"
     CDS             1417..2142
                     /gene="istB"
                     /locus_tag="VNR65_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HWJ19602.1"
                     /translation="MTEELEQLLKNLKLRRILEIYEEQLRAADKDDISYSEFVTRLVR
                     AQFQAKQEGALEWRIQRANLPERWTLETFPFTRQPGVNRKQIRGFAELEFIAKAENIV
                     LTGDTGRGKTGLACGLLLKALENGYRCQFVKAQNLFDEMYASLADRSTRQLLKRLARL
                     DVLLIDELGYLNLKPEQSNIFFKLMEERYRQHSTIITTNLVYDEWPNFLGSRPMVEAL
                     LSRLRHYCHTVHIDGPSLRVPQG"
     gene            2154..2486
                     /locus_tag="VNR65_12840"
     CDS             2154..2486
                     /locus_tag="VNR65_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19603.1"
                     /translation="MDDGCGQEEYIRQVLETYRKTPGTAGTIRRPDRVLATQLYQRGV
                     SVSVIENAFVLAAARRLMRPTDALPLGTIRSLAYFLPVIEEVLGMRVSPEYFQHVRRK
                     LERIARER"
BASE COUNT          534 a          762 c          852 g          515 t
ORIGIN      
        1 aatactaccg gagaagaaac cgcatgttga gccaagcaca acgcaccacg attctggagt
       61 tgaacacgca gcaagtgagc aaacgcgaga tcgcgcgggt gctgggaatc tcccgcgtgg
      121 cggtacgcaa agttctgcga tccaactcga cagctctacc ggaactgcac cgaaccgaga
      181 aggcagagcc ttaccggcaa cagatcctgg aacttttcga ccagtgcaag gggaatctcg
      241 tgcgagtcca tgaggagttg gtggccgcag gcgcggagct gtcgtatacc gcgttaacgg
      301 cgttctgccg gcggcacgga atcggctacg cgccgcccac gccgtcgggt ctgtatgatt
      361 tttcgcccgg cgaggaaatt cagcatgaca cgtctcccca cgagctcgag ttgggcggca
      421 agaagcgcaa ggtgcagacc gcgtctgcgg tgctgtgtta ttcgcggatg ctgttcttcc
      481 agtgttatcc gaactttcgg cgtttcgact gcaaggtgtt tctcaccgag gcgttgcgtt
      541 tcatgggtgg cgcgaccact cgcgtgatga tcgataacac gcacgtggtg gtgctgcggg
      601 gcagcggacg cgacatggtg ccggtgccgg agatggccgc ctttggagag cgtttcggct
      661 ttcagttcgt ggcgcacgcg attgggaatg cgaaccgttc ggggcgcgtg gaacggccct
      721 tccatttcat cgagaataac tttctggccg gacgcacctt ccgcagttgg gaggatctga
      781 accagcgggc gcgggagtgg tgcgataagg tcaactcgac ttacaagaaa catatccgtg
      841 cgattccccg tgagctgttc gcagtcgagc ggctgcatct gaagccactg ccggcgtgga
      901 ttccggaggt ataccggttg cagcagcggc tggtggatat cgaaggatat gtggcgctga
      961 acagcaaccg ctattcggtc ccggtggagt ggatcggccg ccgcgtggaa gtgcgcgaga
     1021 ccagggacaa gatcgagatt caactcgatg cccgccgcct ggtgacgcac cggcgcatcg
     1081 ccgaagccga acaccagcga gtgatgctgg ccgagcatcg gccgccgcgc ggtcacagag
     1141 acgcgcgtcc cgatccgcat cccgaagagc gggccatcgt cacggccgca cctgaactgg
     1201 ccgattatgt cgcggggttg aaacaacgca gccgcaaggt ggtcacgctt gccctgcggc
     1261 aactgctgcg cctggtgcgg gagtatccac gagaaccgct gctgggggcg gtgcgcgagg
     1321 ccggccgtta tggtctttac gatctggacc ggttggaaca catgattctg cgccgcgtga
     1381 cgcgcgaata ctttctactc gatggggaag cggacgatga ctgaagaact ggaacaactg
     1441 ctgaagaatc tcaagctgcg gcgcatcctg gagatctatg aggaacagtt gcgcgccgcc
     1501 gacaaggacg acatcagcta ctcggagttc gtgacgcggt tggtgcgcgc gcagtttcag
     1561 gccaagcagg agggcgctct ggaatggcgc atccagcgcg cgaatttacc cgagcgttgg
     1621 acgttggaaa cgttcccctt cactcgccag ccgggagtga accgcaaaca gattcgcggg
     1681 tttgcggagt tggagttcat cgccaaggcg gagaatatcg tgctgaccgg cgacaccgga
     1741 aggggcaaga ccggattggc ttgtggcctg ctgctgaagg cgctggagaa tggctatcgg
     1801 tgtcagttcg tcaaggccca gaacttattc gatgagatgt atgcgtcgct ggccgatcga
     1861 tcgacgcggc agttgctcaa gcgcctcgcg cggctggatg tccttctgat cgacgagctt
     1921 gggtatttaa atctgaaacc ggagcaatcc aacatctttt tcaagctcat ggaggagcgc
     1981 taccgccagc attccaccat tattacaacg aatctcgtgt atgacgagtg gccgaatttc
     2041 ctgggcagcc ggccgatggt ggaagcattg ctcagccggc tgcggcatta ttgccacacg
     2101 gtgcacattg acggtccgtc gttgcgcgtg ccgcaaggtt gaggagcacc atcatggacg
     2161 acggctgcgg ccaggaagaa tatattcgcc aggtcctgga gacgtatcgc aaaacgccag
     2221 gaacggcggg cacgattcgc cgtccggacc gggtgctggc cacgcaactc taccaacgcg
     2281 gcgtctccgt gagcgtgatc gaaaacgcat tcgtgctggc ggcggcgcgg cgcctgatgc
     2341 gtcccaccga tgccctgccg ttaggcacga ttcgttcgct ggcctatttc ctccccgtga
     2401 ttgaggaagt gctcggcatg cgcgtcagcc cagagtattt tcagcacgta cgccgcaagc
     2461 tcgaacgaat cgcccgcgag cggtagggcc gccacgcgcg ccattgtcgc ggatgatgga
     2521 acaccaaccc gcacgcctca ggcgtggatg atactgcttg ctcgtttcac tcccacaaga
     2581 acacgccttc ggcgtggatg atggccccct gatctcgctc ccgttcccag tcccgttctc
     2641 agagtgggtc acttcagccg aga
//
LOCUS       DATMGX010000783         2424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_357718, whole genome shotgun sequence.
ACCESSION   DATMGX010000783 DATMGX010000000
VERSION     DATMGX010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357718"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1036..1317
                     /locus_tag="VNR65_12845"
     CDS             1036..1317
                     /locus_tag="VNR65_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19604.1"
                     /translation="MENNGKKTSRREILKLGAYGLGAIGAMSVASSANAQGQVAKKAA
                     QKAVMYQQTPKDGKQCDGCQHFQAPSSCRIVDGEINPNGYCILFAKKPA"
     gene            1527..>2424
                     /gene="ccmI"
                     /locus_tag="VNR65_12850"
     CDS             1527..>2424
                     /gene="ccmI"
                     /locus_tag="VNR65_12850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HWJ19605.1"
                     /translation="MIWLIFAVLTACVVLWITRSLRRASSSGNTRSSEIEAYKLQLNE
                     LDREEERGTLGKAEAQQTRDEVSRRLLRASRQSVEAAPKKPRILSAAISFIAVAAVVS
                     LGSIGTYVYYGKPGIPDQPLEARLSAPLGEQSLDVQVANMERRLRQNPADVTGWAALA
                     SIYFKSGVFEKAADTYQRAIDAGGDNEALLLGLAESLTFANDGFISEQAAQALQTALK
                     RNPSSTRGRFWSALLAEQDGKKAEAERIYRDLLAAQIHPTLRNIVTQRLEALSAVADN
                     GTASEERKSAAQDALSDRGEEQK"
BASE COUNT          588 a          650 c          648 g          538 t
ORIGIN      
        1 atcgcgtcat tcgatgcacg gaatatacgg acctaactgt gtgtcgattg tcagttcgca
       61 aacaagctca attgctaaac ctgtctgtaa gttcgcagct ttacgataat cctttctggt
      121 ttctgatgta cagcggataa attttgtagc ttcaaaaata agccatcatt cacgcttatt
      181 gttccataaa cgatatatta atctttttaa taaaaaaata gcttttggta tattgtattt
      241 tactttttat acttcagctt aactataata ctctctcggt atagctagtg cttctggcgc
      301 ctcgttcaaa gacggcgatt atcctcgcac ccaagattcg cgtaaggcct gccctcgccg
      361 ggtatgcatt tcgccgtgag atttttgatt ataatcaaga cgccgcttcc tgcgtcagtt
      421 tgactgtgct tcgctcagag attgtggcag ggcgaggaag gtcggagagt ccggtgtatt
      481 aaccataggg gggccaccaa atgatgcctc ctgatcgaga acatgccttc ggttattaag
      541 ttgctcaaag acttcaaaaa tattaagaat atcaccttta gatataaatg acggaaagaa
      601 acaactccaa atcgaaagag ttgggcatta acatacccta agcttagaag gttaccttaa
      661 acttccgcta gaactggcaa tacgagtggc aggcttgcac tcttcctgca gccagtgatt
      721 gcattggatg cgtcttagca ctttgcggcg gcactccatg aggctccggc accttgcttc
      781 ttacccaagt acaagttagg ctctgaccgg tcaccgagct tggacgggga cgtcttctca
      841 gatgtcaaat acggtcgcag cctcccgcca atgagaaatg cagaacagac tcaccagcag
      901 ttgacgatgt ggcaaggcca cttgagccag ggcgttcttg tggcgaaagc ctcgttcgcg
      961 gatcgaaggt gacatatcga taaggaccgg aaaacggcca aggaagacca gcaacttaag
     1021 aatcaaggac gacccatgga aaacaacggc aagaagacct cgcggcgcga aattctcaag
     1081 ctcggcgctt atggcctcgg cgctatcggc gcaatgagcg tcgccagcag cgcgaatgct
     1141 caaggccagg tggcaaagaa ggccgcgcag aaagcggtca tgtaccagca gaccccgaaa
     1201 gacggcaagc agtgcgacgg ctgccagcat ttccaagccc cctcttcgtg ccggattgtt
     1261 gacggcgaga tcaatcccaa cggatattgc attctcttcg ccaagaagcc tgcttaagcc
     1321 agcaggattc tccgaaagct gcggcttgat cgcagcagcg atagcgatca ttcaggcggc
     1381 ttcatcgaac cattgagccg cctctttata tttcggggcc gcgaacgcgg tctcccgatg
     1441 gatccgcatt ccgcttttcc tcttatccga atggtaacct tggaccgtct tgactcccta
     1501 ggcgggcata gtagtgtcgc accgcgatga tttggctgat atttgcagtg ctgacggctt
     1561 gcgtggtcct ctggataaca cgctcgcttc gtcgcgcctc atcgtctgga aatacccgat
     1621 cttccgaaat cgaggcatac aagcttcagc tcaacgagct tgaccgtgaa gaagagcgcg
     1681 gcaccctcgg caaggcggaa gcgcagcaaa cacgagacga ggtgtcgcgc cgattgctga
     1741 gagcgagccg gcaaagcgtc gaggccgcgc caaaaaaacc aagaatttta agcgccgcca
     1801 tttcctttat cgccgtggcg gcggtggttt ccctcgggtc gatcggcact tacgtttatt
     1861 atggcaagcc cggcattccg gatcagccgc tggaggcgcg tctgagcgcc ccgctcggag
     1921 agcagagtct cgatgtccag gtcgccaata tggagcgccg cctgaggcag aacccggcag
     1981 atgtaaccgg ctgggcggct ctggcgtcca tctattttaa gagcggtgtg tttgagaagg
     2041 cggctgacac ttaccaaagg gcgatcgacg caggcggcga caacgaagcc ctccttctcg
     2101 gtctcgccga atcgctgact ttcgccaatg acggcttcat ctcggagcag gctgcccaag
     2161 cgttgcaaac ggcactcaag cgcaatccga gctcgacgcg cggccgcttc tggagtgcac
     2221 tcctcgctga gcaggatggg aagaaagctg aggcggagag gatataccgc gacctgcttg
     2281 ccgcacaaat ccatccgact ttgaggaaca tcgtaacaca gcgcctggaa gcgctttcgg
     2341 cggttgcgga taatggcacc gcctccgaag agcggaaatc cgccgctcag gatgcgctgt
     2401 cagatcgcgg cgaggaacag aaga
//
LOCUS       DATMGX010000784         2803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_430256, whole genome shotgun sequence.
ACCESSION   DATMGX010000784 DATMGX010000000
VERSION     DATMGX010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430256"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..>2803
                     /locus_tag="VNR65_12855"
     CDS             75..>2803
                     /locus_tag="VNR65_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HWJ19606.1"
                     /translation="MNRLLRGATDTTFLAVGGGLPLVPLGVRYVITVDTDTRLPIGAA
                     KRLIGKMAHPLNRPHVDPARRRVVEGYGVLQPRVTPSLPTARDGSIYQRVFSSPTGID
                     PYAAAVSDVYQDLFEEGSYSGKGIYDVDAFEAALAGRIAESAVLSHDLLEGIFARAGL
                     VSDVEVFEDFPTRYDAAASRQHRWARGDWQLLPWILGRGSATDREIDGAGIPLLGRWK
                     LVDNLRRTLSAPAGFLALLTGWVSLPPAAALLWSGFGLLPFLLTAFLPLVARVYPHRS
                     GITPGSHLRAFRQDLRVALLQFGLQVALLAHQAWLMVDAIVRTLSRLHRRRQLLEWVT
                     AAAASSDRELNLAGFYRRMAGAVVLAAGSTIAVAYADHGTWVIAAPLIAAWALSPAIA
                     WWVSGAPRPDQKSLSDAESESLRLVARRTWHFFETFVTAEDHMLPPDNFQEDPRPVLA
                     HRTSPTNLGLYLLSVLAARDFGWLGLTSTVDRLEETLRTMDGLDRFRGHFFNWYDTRD
                     LHPLEPKYISSVDSGNLAGHLIVLRNACREMISAPVIGPQALAGIADAVTLARISLDA
                     VRSNHQAQDIARKQLQTDLESLGASLKSSAGSLEDFVNRLSNLKREAGGLPDLARILV
                     EEGEGFVSGKADVLTWTEAVVSSVQCHQRDIQGLLPWIQFTADATVWSAALTLEDLPD
                     RCQILIEKLGQQRAALTSMNAAEDTAQIDGRIALLKQSAANAKALVRRIEALNESAAK
                     MFDEMEFGFLLDPARQLLSIGYQSAEGALDPSCYDLLASEARLASFIAIAKGDIAPKH
                     WFKLGRAVTPIDRGSALISWSGSMFEYLMPELVMREPGGSLLHQTARLIVNRQMEYGA
                     QLGVPWGISESAYNFRDLELTYQYSNFGVPGLGLKRGLSENIVVAPYATALAS"
BASE COUNT          507 a          815 c          844 g          637 t
ORIGIN      
        1 atcgccgacg ggtctggaac gagggccaga gcaagtggat cggatgggaa cgcaagcgag
       61 gaaaactgca tgaattgaac cgactgcttc gcggggccac ggacaccact ttcctggcgg
      121 tgggcggcgg cctgccactc gtacccctcg gagttcgtta cgtcattacc gtggacactg
      181 acacgcgcct gcccattgga gcggcaaagc gcctgatcgg gaaaatggcg caccccctca
      241 atcgaccgca cgtggatccc gcgcggcgcc gtgtggtcga gggatatggc gtgctgcagc
      301 cccgggtgac gccatctctt cctaccgcgc gggatggctc aatttatcag cgcgtgttct
      361 cgagcccgac cggcatcgac ccatatgcag cggccgtttc agatgtttat caagacctgt
      421 tcgaagaggg ctcctacagc ggtaagggta tttacgatgt ggatgccttc gaggctgcgc
      481 tcgcaggacg catcgcggaa agcgccgttc tcagtcacga ccttttggag ggcatttttg
      541 ctcgcgcggg gctggtttca gatgtcgagg tctttgaaga ttttccgacg cgttacgacg
      601 ctgctgcgtc gcgccaacac cggtgggcgc gcggcgactg gcagttgctt ccttggatct
      661 tgggcagggg gtccgcgacg gatagagaaa ttgatggggc gggaattccg ttacttggcc
      721 gctggaaact cgtagacaac cttcgtcgta ctctgtccgc cccggcaggt tttctcgctc
      781 tcttgaccgg atgggtctcg ctgcccccgg cggccgcctt gctctggtcc ggcttcggcc
      841 tgcttccttt cctgttaacc gcattcctgc ctctggttgc ccgcgtgtat ccgcaccgct
      901 cggggatcac gcccggcagc catctccgcg catttcgtca ggatctgaga gtcgccttgt
      961 tgcagttcgg cctacaagtg gcgctgctcg cacatcaggc ttggctgatg gtcgacgcca
     1021 tcgttcggac gctgtcgaga ttacatcggc gtcgacaatt gctggagtgg gtgactgccg
     1081 cagcggcaag ctccgatcga gaactcaacc ttgccggctt ctatcggcga atggccggcg
     1141 ctgtcgtgtt ggctgccggc agtaccattg ccgttgcata cgccgaccat gggacttggg
     1201 tgatcgccgc tccgttgatc gctgcttggg cactgtcacc ggcaatcgca tggtgggtga
     1261 gcggggcgcc caggcccgac cagaaatcgc tgtcggatgc ggaatcggaa agtcttcgcc
     1321 tcgttgcgcg gcgcacttgg catttctttg aaacattcgt tacggcggag gatcatatgc
     1381 ttcctccgga caattttcag gaggatcccc ggccggtcct ggcccaccgc acctcgccga
     1441 caaatcttgg cctctatctt ctttcggtcc ttgccgcgcg ggactttggc tggctcggcc
     1501 tcacgtcgac ggttgatcgg ttggaagaaa cgcttcgtac gatggacggg ctcgatcgct
     1561 tccgcggtca tttcttcaat tggtatgaca cgcgtgatct tcatccgctc gagcccaaat
     1621 acatatcatc tgtcgacagc ggtaaccttg ccgggcatct gattgtgctc cgcaatgcct
     1681 gccgagagat gatctcggct cctgtgatcg gtccgcaagc gcttgccggg atcgctgatg
     1741 cagtgacgct cgctcgaatt tctctggacg ccgtaagaag caatcaccag gctcaggaca
     1801 tcgctcgcaa acaacttcaa accgatctgg agtcgctcgg ggcatcacta aaatcttctg
     1861 ccggctcact tgaggatttt gtcaatcgat tgtcgaacct gaaacgagag gccggtggac
     1921 ttcctgacct cgcccgaatt cttgttgagg aaggggaagg cttcgtttcc ggcaaagcgg
     1981 atgtgctgac ctggactgag gccgttgttt catctgtcca gtgccatcag cgtgatattc
     2041 agggtttgct tccgtggatt cagttcaccg ccgacgccac ggtgtggtcg gcggcgctga
     2101 cattagagga cctaccggat cgttgtcaga tattgattga aaaactcggc caacagaggg
     2161 ccgccctcac atcaatgaac gctgctgagg ataccgcgca aattgacggc cgcatcgcct
     2221 tgttaaagca atcggcggca aatgcaaaag cgctcgtgcg acgcattgaa gctctcaacg
     2281 agagcgcggc aaaaatgttc gacgagatgg aattcgggtt tctgttggat cctgccaggc
     2341 agttgctctc catcggttac caatctgccg agggcgcgct ggatcctagc tgttacgacc
     2401 tgctggcgtc agaggcgcgc ctggcgagct tcatcgccat cgccaaaggc gacatcgccc
     2461 cgaaacattg gttcaaattg gggcgcgcag tgaccccgat cgaccgcggg tcggctctga
     2521 tctcgtggtc gggatcgatg tttgagtact tgatgccgga actggtgatg cgcgagcccg
     2581 gggggagctt gttgcatcag acggcccggt tgatcgtgaa ccggcagatg gagtacggcg
     2641 ctcagcttgg cgttccgtgg ggaatttccg agtccgctta taattttcgc gatctcgagc
     2701 tcacttacca atactccaat tttggcgtcc ccgggctcgg actaaagcgt ggcctcagcg
     2761 agaacatcgt cgttgcaccc tatgcgacgg ctcttgcatc cat
//
LOCUS       DATMGX010000785         4800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_202579, whole genome shotgun sequence.
ACCESSION   DATMGX010000785 DATMGX010000000
VERSION     DATMGX010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202579"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VNR65_12860"
     CDS             <1..693
                     /locus_tag="VNR65_12860"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWJ19607.1"
                     /translation="LMARIFSPYDLNPMGLNPLRQVLAESIDFQRLVTSPIKLFITAT
                     NVHTGRGRIFRNSQITPDVLLASACLPTMFQAVEIEGEPYWDGGYAGNPTITPLVRES
                     DAHDTILVQINPRESRKTPKTAGDILDRLNEISFNSPLMKELRMIALLRQAADPGHGE
                     GARWAGMRTHRIMNDRLAEFGASSKLNAEWEFLAMLKAEGQKSAGDFLTRNGDDLGSK
                     SSADLDILLEEC"
     gene            742..2184
                     /locus_tag="VNR65_12865"
     CDS             742..2184
                     /locus_tag="VNR65_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19608.1"
                     /translation="MSVVGILIALGLLVWLAYRGWTILLLAPLCALIAALLAGQPLLA
                     NWTQTFMSNAASFVAQFFPIFLLGALFGKLMEASGSVEAIAKYLTERLGTKRAMLAIV
                     LAGAFVTYGGVSLFVAFFVLAPMGHALFKAANIPHRLMPAAIALGTSTFTMSAMPGTP
                     AIQNAIPMPFFGTNPFAAPGLGLTASAIMLGFGLWWLARAEAKARRAGEAYDYAHPTD
                     PAAAAADGIVRERATTAREFDPEEIARGARAAALPPFVLAALPLVTVVSVNLIMSFGV
                     LPNIDASFLNETQWGATSLAAVGGVWSVIVALAVAIVVLIATNFNRLPTLRATMDAGA
                     NAAVLPIISVASLVGFGAVVASLPAFEAVKAWVLGIEGGPLVSLAVATNILAALTGSA
                     SGGLTIALDALGPTYMQTGLDPGLMHRVAVIGAGTLDSLPHNGAVVTLLAVCGSRHRE
                     SYGDIVMAAIVGPIVALAAVIALGSTLGSF"
     gene            2698..3162
                     /locus_tag="VNR65_12870"
     CDS             2698..3162
                     /locus_tag="VNR65_12870"
                     /inference="COORDINATES: protein motif:HMM:NF017950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HWJ19609.1"
                     /translation="MRKICFATILCAAMVVFIPALAQAQPTRVGGLTCDAAPRVGLVV
                     GSRQQLRCVFSSNAAGHCPYSGTIGRIGLDIGVTGGGRLVWAVFAPTSHVGCGALRGS
                     YVGASGSASLGVGLGANVLVGGSHRTISLQPLSVEGQLGVNLALGVSRLTLR"
     gene            complement(3696..>4800)
                     /locus_tag="VNR65_12875"
     CDS             complement(3696..>4800)
                     /locus_tag="VNR65_12875"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ19610.1"
                     /translation="RVIVLICRIRLLTTAALASAIFCSGCSGRLAQGELVPVAQVDGE
                     GISLVPVLVVTTRSRSTADAGEMFNRRPAEEVSYASVTVSIPPDILRRTGEVQWPASV
                     PGDPRQSFVTVSANVLDRQSFVSDISAATKRNGRGKVLVFVHGFNNRFDRAVYRFAQI
                     THDSRAPAVPVLFSWPSRGIAGLRAYQEDVGSANASRDAIEQLLDTIGKNPNVREVTV
                     LCHSMGCSPTLLALHARAQRAGSIGSKVKNVMLVAPDVDADLFRTQMRDMGSTRPRFA
                     LFVSQDDVALKLSASIWGGTTRLGQLDPDQEPYKTDFRREGVLVFDLTGMRGRAHSRA
                     FREVNSVMGMIEQRFAEGQQMTDTGSEPEAASQ"
BASE COUNT          935 a         1431 c         1413 g         1021 t
ORIGIN      
        1 ctgatggcgc gcatcttctc accttacgac ctcaacccaa tgggtctcaa cccgcttcgg
       61 caagtactag cggaaagtat cgatttccaa cgacttgtaa catctccgat caagctattt
      121 atcacagcta caaacgttca caccggccgc ggacggatct ttcgcaacag ccaaatcact
      181 cccgacgtac tgctggcctc ggcgtgcctt cctactatgt tccaggctgt cgagatcgaa
      241 ggcgagcctt attgggacgg cggctatgca ggtaatccta ccatcacgcc tctggtccgc
      301 gagagcgacg cgcatgatac catcctggtc cagatcaatc cacgagaaag ccggaagacg
      361 ccgaagacag cgggcgatat tctggaccgc cttaacgaaa tttccttcaa ctctccactg
      421 atgaaggaat tgcgcatgat cgcgctgctg cgccaggctg cagatcccgg acatggcgaa
      481 ggggcacgct gggcgggcat gcgcactcac cggatcatga acgacaggct agccgaattc
      541 ggcgcgtcat caaagctcaa tgccgaatgg gaattcctcg caatgctaaa ggccgagggc
      601 caaaagagcg ctggcgactt cctgacccgt aacggcgatg atctcggcag caagtcgagc
      661 gccgatctcg atattctgct cgaggagtgc taagagcatg agtatttttt tgacgctttc
      721 tcaccatgac gttgcgacgc gatgagtgtc gttggaatcc tgatagctct aggtctgctg
      781 gtctggctcg cctatcgggg gtggaccatt ctcctgctcg caccgctgtg tgccctgatt
      841 gctgcgcttt tggcgggaca gccgctgctg gcaaactgga cgcagacttt catgagcaac
      901 gctgcaagtt ttgtcgcgca gtttttcccc atcttcctcc ttggcgcgtt gttcggcaag
      961 ctgatggaag ccagcggttc ggtcgaggcg atcgccaaat atcttaccga gcgcttgggc
     1021 accaagcgcg ccatgcttgc catcgtgctg gcgggagcat tcgtcaccta tggcggcgtc
     1081 agcctgtttg tcgcattttt cgtgctggcg ccgatgggcc acgccctgtt caaggctgcc
     1141 aatataccgc atcgcctgat gcccgcggct atcgctctcg gcacctcgac cttcacgatg
     1201 tcggcgatgc ccggcacgcc tgcgatccag aacgcgatac cgatgccgtt tttcggtacc
     1261 aacccgtttg ccgctcccgg cctcggcctc acggcatcgg cgatcatgct cggatttggc
     1321 ctttggtggt tggcgcgggc ggaagctaag gcgcggcgcg ccggcgaggc ctatgactac
     1381 gcccatccga ccgatcccgc cgctgccgcg gccgacggga tcgtccgcga gcgcgccacc
     1441 acggcgcgag aattcgatcc cgaggagatt gccaggggcg ctcgcgccgc cgcgctgccg
     1501 ccctttgtgc tggccgcgct tcccctcgtc accgtcgtat cggtcaattt gatcatgtcc
     1561 ttcggggttc tcccgaatat cgacgcctcc tttcttaacg aaacgcagtg gggcgcgacg
     1621 tccctcgctg cagtcggcgg cgtctggtct gtcatcgtcg cgctggcggt ggctatcgtg
     1681 gttttgatcg ctaccaattt caaccgcttg cccaccctgc gggcgacgat ggacgccggg
     1741 gcaaacgccg cggtgctacc aatcattagt gtggcaagct tggtcggttt cggcgcggtg
     1801 gtggcatcgt tgcctgcctt cgaagcagtg aaggcatggg tgctcggcat cgagggcgga
     1861 ccgctggttt cgcttgcggt ggcgaccaat attctcgccg cgctcacggg ctcggcatcg
     1921 ggtggcctga ccattgccct tgacgcactc gggccgacct acatgcagac cggcctcgat
     1981 cccggcctga tgcaccgcgt ggccgtgatc ggcgcgggta cactcgacag cctgccgcac
     2041 aatggtgcag tggtcacgct gcttgctgtt tgtggatcga ggcaccgcga gagctatggc
     2101 gatatcgtca tggcggcgat cgtcggcccg atcgtcgcgc tagccgccgt aatcgcgctg
     2161 gggtccacgc tcggctcgtt ttaatctact gcaagtccat ttctgggcga gcccgacgta
     2221 ggccaactag tgacccggag gcggcagatc aacgcatttc cctaagtctg acgccaggat
     2281 ttatctattc tcgacgaaac atttttcgag actgcagctt tcaaggctcg tgaatgacag
     2341 ccgatggcac accaaggatt tcgaccggaa tcgctgctgc cgtgctgctt ctggtagcgg
     2401 tgtactatgg gagcagcgtc cttgcgcctt tggcgctcgc cctcttcgga tagccaggta
     2461 aacctgaaag acagctgaga tgtttgtgtc gttgttgacg ggtcatcccg cgctcaaatt
     2521 aattgacttc gaccaatgta aactgatcta agggaatgct gatggatgaa aatttcccag
     2581 ccggctgcaa gattgcgttt gggagaaagc ggagcgttca gccggcgcgt agatgaggcg
     2641 ccgagcacgg gctcccgaaa atcgaaaagt tgcaggaaaa tgccattgga gggacgaatg
     2701 aggaaaatct gctttgcgac cattctctgt gcggccatgg tcgtcttcat accagctttg
     2761 gcacaggcgc aacccacgcg tgtgggcgga ttgacttgcg atgctgctcc gcgggtgggg
     2821 cttgtggtcg gctcgcggca gcaactgcga tgtgtattta gctcgaatgc agcgggacac
     2881 tgtccttaca gcgggactat tggccgaatc ggactcgata tcggcgtgac cggtggtgga
     2941 aggctggtgt gggccgtctt cgcaccgacc tcgcatgtgg gatgcggcgc gctgcgagga
     3001 agctatgttg gggcaagcgg cagcgcgtcg cttggtgtgg gtctcggtgc caacgtgctc
     3061 gtcggcggat cccaccgcac gatttccctg cagccgctgt cggttgaagg ccagctcgga
     3121 gtaaatctgg cgcttggcgt atcgaggctg acgctgcgat aagcggttgg ggcggcttgc
     3181 caggactttc caagtgcatc cgcgcaccaa caccaattgg tgtgcggatc acgaaaggtg
     3241 attagtaagt tcagcggcaa tattgatgcg ataccggatg cgatcgtccg cttgacgtgc
     3301 ggacccagca actgccgtga ctccagtaat agaaattatg cccgcgccaa taaccataat
     3361 ggccgcgcca gtaagctccc aacgcggcgc cagtgactgc accgatcgcg acacctcggc
     3421 gaccgcctat cgcggctcca acgattgcac ccccacggca gtttgcgcaa ccgcatcctc
     3481 cagcggtatc gtcaaggcca gccgcaacag gaataccgac gttactaccc ggcgcagtgt
     3541 cgacaatgtt tccaatcgct tcttttttag attatgaggc cgtcttttta acagcatgcg
     3601 agttgtaacg ccatgcattt ggtcgcggaa ttctttcgcg cggctcccgg agggagcttg
     3661 gcccgctttg atttcgcaga attttcaggg aaaatttatt ggctggcggc ttcgggctca
     3721 gagcccgtgt cggtcatctg ctggccttcg gcgaaccgtt gctcgatcat gcccatcacc
     3781 gagttgacct ctcgaaacgc ccgcgagtgc gcacgcccgc gcatgcccgt caaatcgaag
     3841 accaaaacgc cttcgcgtcg aaaatcggtc ttgtacggct cctgatcagg atcgagctgg
     3901 ccgagccgtg ttgttccacc ccagattgac gcggatagtt tgagggcgac gtcgtcctgg
     3961 gacacaaaca atgcaaagcg aggtctggtg ctccccatgt cgcgcatttg cgtgcggaag
     4021 agatcggcgt cgacgtcggg tgcgacaagc atcacgttct tgaccttgct tccaatgctg
     4081 ccggcgcgct gcgccctagc gtggagcgct agcagggtgg gagaacaccc catcgaatgg
     4141 cacagaaccg ttacctcccg cacatttgga ttttttccga tcgtgtcgag caattgctcg
     4201 atggcgtcgc gagaggcgtt tgcgcttcca acatcctcct gataggcccg cagtccggca
     4261 atgcctcgcg agggccagga aaacaacacg ggaacagcgg gtgccctgga atcgtgagtg
     4321 atctgcgcaa agcgatagac cgcccgatcg aaccgattgt tgaaaccatg aacaaagacc
     4381 agcaccttgc cgcgtccgtt gcgtttggtg gcggccgaaa tatcggaaac aaaggactgc
     4441 ctgtccaaaa catttgccga aaccgtcaca aaactttgcc ggggatcgcc cggcacggaa
     4501 gcgggccact gtacctcgcc ggtcctgcgc aatatgtcgg gaggaatcga aacggtgacc
     4561 gaggcgtacg acacttcttc cgcaggtctc ctgttgaaca tttcaccggc atcggccgtc
     4621 gagcggcttc gtgtggtcac gacgagcacc gggacaagcg atatcccttc cccgtcgacc
     4681 tgtgcgaccg gaaccaattc gccctgcgcc agtcgaccac tacatccgct gcaaaaaatc
     4741 gcggaagcta aagcggctgt agtcagcaaa cgaatcctgc agatcaaaac aatcactcgc
//
LOCUS       DATMGX010000786         3880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_140445, whole genome shotgun sequence.
ACCESSION   DATMGX010000786 DATMGX010000000
VERSION     DATMGX010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140445"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VNR65_12880"
     CDS             <1..483
                     /locus_tag="VNR65_12880"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWJ19611.1"
                     /translation="SVAPLGLIGVVVALLLSDKPLGFVAILGVLALTGIIIRNSVILV
                     AQIDEMSRDGLEPWAAVVAATKHRMRPILLTASAASLGMIPIAPQVFWGPMAFAMIGG
                     IFAATFLTLLFLPALYVGWYGIHEVQSAKRTTPMAAAESGARASTRIDSEPARDEPHL
                     "
     gene            complement(530..1117)
                     /locus_tag="VNR65_12885"
     CDS             complement(530..1117)
                     /locus_tag="VNR65_12885"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HWJ19612.1"
                     /translation="MRSEKVFSLVLKTLLKVSGLMASSWVVPWCVYRLHGIRLIGPPG
                     EEIWYFAYGANMHDSAFREWRGMRPREWRPGRVRGYRLRFNLMAPRGTATYANLCADP
                     AAEVWGVLYKITRRDLVRLEATEGVPWGGYRPHWLDVEDLGGTVLQAATLMAQGHDDD
                     RTPSLRYVTLLREGARAHGLPDHYIRFLEQVEHAV"
     gene            complement(1308..2243)
                     /locus_tag="VNR65_12890"
     CDS             complement(1308..2243)
                     /locus_tag="VNR65_12890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HWJ19613.1"
                     /translation="MTERRGEKAAAPDSKLPFPASEYELPAGQKSPDLVRAERTVWRL
                     SHIHFGWDINKALEFALLNTFAVPSISGLLDRTGEFSSRTLKRYDDTAILIREALSNG
                     LDSDRARRAFARMNDMHGRFRITNDDFLYVLSTFVFTPIDWLDRFGRRAMSDEEKEDW
                     FLYWREFGRRMGIANLPTDIGAFRHLAKEFERKRFAQAPSNRAVAQPTIDLLLGQYFV
                     PPFFYPAGRAFVMALCSPHLVEALGFPAPSSALRLIADGAMGLRRTILRLLPEGTRPK
                     YIRFGKQTYPEGYTIEELGTFQLNTNHHKSGRFRP"
     gene            complement(2248..3474)
                     /locus_tag="VNR65_12895"
     CDS             complement(2248..3474)
                     /locus_tag="VNR65_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWJ19614.1"
                     /translation="MNDSHLKANAAGLIFNPMSPDFLRDPYPTYHRLRRDDPMHRSPL
                     GFTMVSRHADVAFISRDARFGKDVAARVKRTSGEKALEEPVYQSMLRWMMSQNPPEHT
                     RLRGLVVKAFSNRAVEDMRPRIQQIVDQTIDRVASQGHMDLIADFAFRLPVTVISEML
                     GIPEEDQGMVFKGEGPGARLLDPVPLSRAELDAANAGHLAVAQYFHALFELRRRKPAN
                     DIITLLVQAEEEGSKLSNEELTANIILLYVAGHVTTVHSIGNALLALHRNPDQLELYK
                     RNPLLATALDELLRYESPVQVSGRAALEDVSVGGLDLKKGDTILCLLGAANRDPAVYA
                     DPDRLDITRENVRPLSFGGGVHYCLGAHLARIETEIAIATLLRRLPNLTLDHPDAPDW
                     RQTFVFRGLKTLPASW"
BASE COUNT          785 a         1094 c         1162 g          839 t
ORIGIN      
        1 agcgtggcgc ccctcgggct gatcggcgtc gtggtcgctc tcctgttatc tgacaagccc
       61 ctgggttttg tggccattct cggtgtgctc gccctcacag gaattatcat tcgcaactcc
      121 gtcatcctcg tcgcccagat cgatgaaatg agtcgggatg ggctggagcc ttgggcggcg
      181 gtggtggccg ccaccaagca ccgtatgcgc ccgatcctgc tcacggcgtc cgctgcgagt
      241 cttggcatga tccccatcgc cccgcaggtg ttctgggggc cgatggcatt tgcgatgatt
      301 ggcggcatct tcgcggcgac cttcctgacg ctgctctttc tgccggctct gtatgtgggt
      361 tggtacggca ttcatgaggt gcaatcggct aaacgaacca cgccaatggc ggccgctgaa
      421 agtggcgctc gtgcttcaac gcgaatagac tcggaaccgg ccagggacga gcctcacctc
      481 taaaccgccc agaggaacaa gacgggcgag aaccgtggta tccgtccggc tataccgcgt
      541 gctcgacctg ctccaggaag cgaatgtaat ggtcgggcag gccgtgcgct cgtgcgccct
      601 cccgcagcaa tgtgacgtat cgaagcgatg gggtgcggtc gtcgtcatgg ccttgggcca
      661 tgagagtagc ggcctgaagc accgtgccgc caaggtcttc gacatcgagc caatgcggcc
      721 gatatcctcc ccatgggacg ccttcggtgg cctccagacg aacgaggtcg cggcgggtga
      781 tcttgtaaag gacgccccaa acctccgcgg ccggatcggc gcataaattg gcataggtgg
      841 ccgtgcccct gggcgccata aggttgaagc gcagccggta tcctcggacg cggcccgggc
      901 gccactcgcg aggacgcatg ccgcgccact cgcgaaaggc gctgtcatgc atgttcgccc
      961 cgtaggcgaa ataccagatt tcttcgccgg gcggtccgat gaggcggatg ccgtgcaggc
     1021 gatacacgca ccagggcacc acccatgagc tggccatgag tccactgacc ttgagcaggg
     1081 tcttgagcac cagactgaaa accttttcgc tcctcaccta tcttgccctc gccttgcgag
     1141 acattggttc ccctcttctg ccaatcatcg gaggggcgac gcatatctta aattggagct
     1201 tttccaggcg cctcatcctt taagacttac aaacggttaa aattggtttc gttttgtaaa
     1261 acgggcataa gtaaatatat aagtagtatt gtggcgaccc agggcggtca tgggcggaag
     1321 cgtcctgact tgtggtggtt tgtattaagc tggaacgtgc caagctcttc gatagtgtag
     1381 ccttccggat atgtctgctt gccgaaccgg atgtactttg gccgcgtacc ttccggaagc
     1441 agcctcagta tcgtccgccg cagccccatg gcgccgtcgg caatgaggcg cagggcggac
     1501 gagggggcgg gaaatcccag cgcctcaacg agatgcggcg aacacagcgc cataacgaac
     1561 gcgcgtcccg ccgggtaaaa gaacggcggc acgaagtact ggcccagcag aaggtcgatg
     1621 gttggttgcg caaccgcccg gttggatggt gcttgcgcga agcgttttct ctcgaattct
     1681 ttcgccaaat gccgaaatgc gccgatatct gtaggcaaat tggcaatgcc catgcgccgg
     1741 ccgaactccc gccaataaag gaaccagtcc tctttctctt cgtccgacat tgcgcgccgg
     1801 ccgaacctgt ccagccaatc aatgggggta aagacgaagg tcgagagcac gtagaggaaa
     1861 tcgtcgttcg tgatccggaa gcgcccatgc atgtcattca ttcgggcgaa tgcgcgcctc
     1921 gcccggtcgc tgtcgagtcc gttgctcaag gcttctctta tgaggatcgc cgtgtcgtcg
     1981 tagcgtttga gcgtcctgct ggagaattcg cccgtccggt ccagaaggcc ggaaatagac
     2041 ggtacggcaa aggtattgag aagagcaaac tcaagcgcct tgttgatgtc ccagccaaaa
     2101 tggatatggg agagccgcca gaccgtgcgc tctgcacgga cgaggtccgg agatttctgc
     2161 ccagccggga gttcgtattc gctcgcggga aagggcagct tcgaatcggg agccgcagcc
     2221 ttttcccctc gccgctctgt cataacttta ccagctggct ggcagggtct tgagaccgcg
     2281 gaacacgaat gtttgacgcc aatccggcgc gtcgggatga tcgagcgtta ggttcggcaa
     2341 acggcgcaaa agcgtggcta tggcgatttc ggtctcaatg cgggcaaggt gagcgccaag
     2401 gcaatagtga acgccgccgc cgaatgacag gggcctgacg ttctcgcgcg tgatgtcgag
     2461 ccggtctgga tcggcataga cggccgggtc gcggttggcc gcgccaagaa ggcacaatat
     2521 cgtatcgccc ttcttaaggt ccaaaccgcc cacgctcacg tcctcaagtg ctgcacgccc
     2581 gctgacctga acaggggatt cgtatcgtaa aagctcatcc aaagctgtcg ctagcaatgg
     2641 attgcgcttg tagagttcga gctgatcggg attgcgatga agcgccagaa gggcattccc
     2701 aatggaatgg accgtggtga catgcccggc cacatataaa aggatgatgt tggcggttaa
     2761 ttcctcgttg gacagtttgc tgccctcttc ttccgcctgc acgagcagtg ttataatatc
     2821 gttagccggt tttcgtcgcc gaagctcgaa gagggcgtgg aaatattgcg ccacagccaa
     2881 gtgcccagca ttggcagcgt cgagttctgc gcgcgatagt gggacaggat cgagaagccg
     2941 cgcacctggg ccttcgccct tgaaaaccat tccttggtct tcttcaggaa tgcccagcat
     3001 ctcgctgatc acggtgaccg gaagacggaa ggcgaaatcg gcgatcaggt ccatgtggcc
     3061 ttgcgatgcc acacggtcaa tcgtctggtc cacgatctgt tgaatgcgcg gacgcatgtc
     3121 ctccacggcg cgattgctga aggctttgac aaccagacct cgaaggcgcg tgtgttcggg
     3181 cgggttttga ctcatcatcc agcgcagcat ggactgatag acaggctctt cgagtgcctt
     3241 ctctcctgag gtccgcttga cccgggcggc aacgtctttc ccgaagcgcg catcccgaga
     3301 gatgaacgcg acatcggcgt ggcggctcac catcgtgaag ccgagcggcg accggtgcat
     3361 cgggtcgtcg cggcgcaagc gatggtaggt tggatagggg tcgcggagga aatccggcga
     3421 catgggattg aagatcaacc cagccgcgtt ggccttaagg tgtgagtcgt tcacagcatc
     3481 gctcgcggtt gggtgttagg ataaccgaat tctatgataa actagcgcag ctcttttgat
     3541 gatttgaagt aaaattctcg tgcctgtttg attggcactg aacaaactct tgttttcgat
     3601 gtccgcgcag gccgggaaac cgctaacttt cctttatgct cgccggacaa aggcttagta
     3661 taggtcaacc cagtttcata agcgctggcg accttgacct cgtgctccgc aagccgttcc
     3721 accttgtgtt gtttgtgcaa ctactgcgta tgaatttttc tgtgtgcacg tataagctgt
     3781 tgcctaggca ttcagttgct aatatattga taccagcagg atatcagttc tgccagttct
     3841 ccaaagacgt gttctatgca aatattagct tgccgacgcc
//
LOCUS       DATMGX010000787         2441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_473572, whole genome shotgun sequence.
ACCESSION   DATMGX010000787 DATMGX010000000
VERSION     DATMGX010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473572"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VNR65_12900"
     CDS             <1..1041
                     /locus_tag="VNR65_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWJ19615.1"
                     /translation="SKQLGLGTNILDAGALGDTKKQESSQMAEAIEKADGFAQVFPEH
                     KFHIIDVLQKRGHIVGMTGDGVNDAPALKKADCGIAVSGATDAARAAAAIVLMTPGLS
                     VIIAAIKESRKIFQRMNSYAIYRIAETLRVLLFMTLAILVFNFYPLTAVMIVMLALLN
                     DGAIPSIAYDNVHYKDQPEAWNMPLVLGIATALGIIGVVSAFGLFYLGERVFHLDRAH
                     IQTLMYLKLSVAGHLTIFLTRTRGPFWSIRPARILWGAVLGTQIVATLIAVYGLFMTP
                     LGWGWALFVWGYALAWFLVNDRVKLLAYRFLDPVKVERKPAAKAEPKPDAEPKREARA
                     KTPSDSTPQAAK"
     gene            1121..1990
                     /locus_tag="VNR65_12905"
     CDS             1121..1990
                     /locus_tag="VNR65_12905"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HWJ19616.1"
                     /translation="MKIDSKDFRVREGDKINLKKWPTIVKPVYKSKEQYKKLLEEHVA
                     QLSLQQQLLYASNRHAVLLIFQAMDAAGKDGAIRHVMSGVNPQGCQVFSFKHPSAAEL
                     EHDFLWRTTRDLPERGRIGIFNRSYYEEVLIVRVHPEILRSEAIPDTPHHDRKIWHDR
                     YRSIVDLEKHLHANGTRIVKFYLHLSKEEQRTRFLARIDEPDKNWKFSLADIAERKFW
                     TQYMKAFEECLSATSTQDSPWYVVPADDKENARLIVSQIVIDTFEGLKMAYPKTSAKR
                     RKELQSIRERLVK"
     gene            2100..>2441
                     /locus_tag="VNR65_12910"
     CDS             2100..>2441
                     /locus_tag="VNR65_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19617.1"
                     /translation="MSKKPSVKSSELSLMSVPAFWPMAMAAAMMEEGTELYAKNVKFA
                     EEEIKIHDELRPKLATPNQIRIDLRTMVLRDYGKPGGLPTLVDAPHAGHTAMIADYHK
                     GQSLVETLLANG"
BASE COUNT          574 a          681 c          704 g          482 t
ORIGIN      
        1 tcgaaacaac tcggactggg cacaaacatc ctggatgctg gtgcgctggg tgacacgaag
       61 aagcaagagt cttctcagat ggcggaggcc atcgaaaagg ctgacggttt tgcccaagtg
      121 ttcccggaac acaagttcca catcatcgac gttctgcaaa agcgcggcca catcgtcggc
      181 atgacgggcg atggggtgaa tgacgccccc gcgctgaaga aggccgactg cggcatcgct
      241 gtctcgggcg cgacggacgc ggcgcgcgcg gcagcggcca ttgtactgat gacgcccggc
      301 ctgtcggtga ttattgctgc tatcaaagag agccgaaaaa tcttccagcg gatgaacagt
      361 tatgcgatct accgcatcgc cgaaacgcta cgcgtgctgt tgttcatgac gctggcgatc
      421 ctggtcttca acttttatcc attgacggcg gtgatgatcg tgatgctggc gctgctcaac
      481 gacggcgcga tcccgtccat tgcctatgac aacgttcatt acaaggatca gccagaggcc
      541 tggaacatgc ccctcgtgct ggggatcgcc acggcgctgg gcatcatcgg agtcgtctcc
      601 gcgttcggcc tgttttatct tggcgagcgc gtatttcacc tcgaccgcgc acacatccag
      661 acgctgatgt acctgaagct gtccgtggcc ggacatctga cgatcttcct gacgcgcacg
      721 cgcggcccgt tctggtcgat acgcccggcg cggatcttat ggggcgcggt gctcggcaca
      781 caaattgtgg caaccctgat tgcggtttac gggctgttca tgacgcccct cggctggggt
      841 tgggccctgt tcgtctgggg ctatgcgctt gcctggttcc tcgtgaacga ccgcgtgaaa
      901 ttgctcgcgt accggttcct tgatccggtc aaagttgagc gcaagccggc agccaaagcc
      961 gagcctaagc ccgacgccga gcctaagcgt gaagccagag ccaagacgcc gtccgattcg
     1021 acgccgcagg ccgctaagtg agctgatgaa tgttgcggca acgccgtcgc gaaaatgaat
     1081 cgacgcggcc catgcggcca gcacgactga aaggaagaag atgaaaatag actccaaaga
     1141 ctttcgcgtg cgggaaggag ataagatcaa tctcaagaag tggccgacga tcgtgaagcc
     1201 cgtatacaag tcgaaggagc agtataaaaa gctattggaa gaacacgttg cacaactaag
     1261 tttacaacag cagcttctct acgcatccaa ccgccatgct gtcctgctga tctttcaggc
     1321 gatggatgcg gcgggaaaag atggcgccat ccggcatgtg atgtccgggg tgaatccgca
     1381 aggctgccag gtcttcagct tcaaacatcc cagcgccgcc gaactggaac acgattttct
     1441 ctggcgcacc acgcgcgatt tgccggaacg tgggcggatc ggcatcttca accgatccta
     1501 ttacgaggaa gtgctgatcg tccgtgtgca tcccgagatt cttcgcagcg aagcgatccc
     1561 ggacaccccc catcacgaca ggaaaatctg gcacgacagg tatcgttcga tcgtggattt
     1621 ggagaagcat ctccacgcca acggaacccg catcgtcaag ttctacctcc acctatcgaa
     1681 ggaggagcag cgaacgcgct ttctggcgcg tatcgacgag ccggacaaaa attggaaatt
     1741 cagccttgca gatatcgcgg agcggaaatt ttggacgcaa tacatgaagg cgtttgagga
     1801 atgtctcagc gcgacaagca cccaggattc gccttggtat gtcgttcccg ccgacgacaa
     1861 agagaatgct cgactgattg tgtcccaaat tgttatcgat acgttcgaag gccttaaaat
     1921 ggcttatccc aaaacgagcg caaagcgtcg aaaggaattg cagtcgatcc gcgagcgact
     1981 cgtgaagtga gatctgttgt atcggtatgg gatctagaac gcgcaaacga aggcaacgga
     2041 ggcacgcgcc cgggttagat gcctgagagc gacccaattc agcaataggt ggaagaatca
     2101 tgtcgaagaa gccgtctgtc aaatcctctg aactgtcttt aatgagcgtg ccggcattct
     2161 ggccgatggc gatggcggca gccatgatgg aggagggaac cgagctttat gccaagaacg
     2221 tcaaattcgc cgaggaagaa atcaagatcc acgacgagct acgccccaag cttgccaccc
     2281 caaatcaaat tcgcatcgac ctgcggacca tggtgctgcg cgattacggc aaaccgggcg
     2341 ggcttccgac gctcgtcgat gctccccatg ccggtcacac agccatgatc gccgactatc
     2401 ataaaggtca gagcctggtt gagactctgc tggccaatgg a
//
LOCUS       DATMGX010000788         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_20164, whole genome shotgun sequence.
ACCESSION   DATMGX010000788 DATMGX010000000
VERSION     DATMGX010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20164"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..2165
                     /locus_tag="VNR65_12915"
     CDS             165..2165
                     /locus_tag="VNR65_12915"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWJ19618.1"
                     /translation="MSVEEAPVWLRHHGGRITTVALILALLYVGRTVLIPLALAIMLS
                     LLVAPLVRTLRRIRVGRTSSVLIAVLALTLSCMAVAVVLGTQILRIAESLPQYQANVQ
                     HKIRVVDEVTVGRMRLLTKEASRLTEIHDPSDTRSGPNGEVKRSVAAQPPGPVLLEPR
                     DLPPQPLQLMGKLLTSAWKPLQATGIVLIVLIFVLLEHESLRDRIIRIAGLTDIRSTT
                     VALNDAGERLSRFFVSQFAVNLAFAVIITVVLSVLRVPQAILCGTLAGVMRFVPYVGV
                     GIAALFATALAFAVDPGWSLAASTLGAFILLDIVAAQLLEPRLYGHATGLSPLSVVVA
                     AIFWSALWGPVGLILSTPLTLCLFVAGRHVKALNILELLLGDVQPLSLPQNFYQRALS
                     ADPHEIIANARAFLKRDSLATYCDRVLLPALHLAQLDAEPGATTENQQMKIRRVIVDV
                     LSAVGSDSLKLPRRRHRGSVLRDVSAGRWLRQERERLSGRWQGPLGVPPGSVVVCAGV
                     GSSFDDLAAELLVRMLRTQTIDARHFSSGDINLGLPPGADPDGVSMVYLVSAFPGAER
                     ARADSLSKQVRELLPRACVVRVFCPGVTALSESGKGAGHTEPTVNCLGQAIEICVSWQ
                     EVRSTRDPAPDLTSLMARERRSALQHSEGLRRRREHTEEVLQ"
BASE COUNT          383 a          703 c          675 g          483 t
ORIGIN      
        1 atgtcaccgc cgtcggaaca atatggcgcg ccgggattca ggcgtcctgg caacgaggag
       61 ccgcccatac tttgtccccg cgctgggctc gaccggctgc acttgaattt gagcgtcgtg
      121 cggcgtgaac aagtccaaca gacatcggag tagaggtaac tagcgtgagt gtcgaggaag
      181 cacccgtttg gctgcgccat catggcggcc gcatcacgac tgtggcactc atccttgccc
      241 tgttgtacgt cggtcggacg gtgctaatac cgctcgcgct cgccattatg ctgagcttgt
      301 tggtggcgcc gctggttcga acacttcggc gcatccgagt cggccggacc tcttcagtgc
      361 ttatcgcagt tctggcgctc acactttcgt gcatggccgt cgcagtggtg ctcggcacac
      421 agattctgcg catcgccgag agccttcctc agtaccaagc aaacgtgcaa cacaagatca
      481 gggtcgttga cgaagtcact gtcggacgga tgcgcctgct tacgaaggaa gcgagccgcc
      541 tgacggagat ccacgatcca tcagacaccc gctccggccc caatggcgag gtcaagcgct
      601 ccgtggcggc acagccaccg ggccccgtgc tgttggagcc gcgcgacctg ccgccccagc
      661 cgctccagct catggggaag ctcctgactt ccgcttggaa accgctccaa gccacaggta
      721 tcgtgctgat cgtcctcatt ttcgtgctgc tcgaacatga atccctgcgc gaccgcatca
      781 tccggatcgc gggcctaacc gatatccgct cgacgactgt ggcgctcaac gacgccggtg
      841 agcgtctctc aaggttcttc gtctcccagt tcgcggtcaa tctggccttc gcggtaataa
      901 ttacggtggt gttgagtgtc ctgcgcgttc cgcaggcaat tctctgcggc acgctcgctg
      961 gggtgatgcg ctttgtacct tatgtgggtg tgggaattgc ggctctcttt gcaaccgctc
     1021 ttgcctttgc cgtcgatccg ggatggtcgt tagcggcgag cacacttggt gccttcatac
     1081 tcctcgacat cgtcgcggca caattgctgg aaccgcgcct ttatggtcat gccaccggac
     1141 tatcgccgct ctcggtagtg gttgcggcca tcttctggag cgccctctgg ggtcccgtcg
     1201 gcctaatact gtccaccccg cttaccttgt gcctgttcgt cgccggacgt cacgtgaagg
     1261 cgctcaatat cctggaactc ctgttgggtg atgtccagcc gctgtcgctc ccccagaact
     1321 tctaccaacg ggcgctttcc gcagaccccc acgaaatcat cgctaatgcg cgagcattcc
     1381 tgaaacggga ttctcttgcg acgtattgcg atcgcgtctt gcttccagca ctgcaccttg
     1441 cgcagctcga tgccgaacca ggtgcgacca ccgagaatca gcagatgaag atccgccggg
     1501 tgatcgtcga tgtcctttca gccgtgggca gcgacagcct caaacttccg cgacggcgac
     1561 atcgaggctc ggtgctccgg gacgttagtg ccgggcgctg gctgcgtcag gagcgcgagc
     1621 gattgagcgg gagatggcaa ggcccactcg gcgtgcctcc cggatccgtg gtggtctgcg
     1681 ctggagtggg gtcatccttc gatgatcttg cggccgagct gctggtacgg atgttgagaa
     1741 ctcagactat agatgctcgg catttctctt ccggagacat aaatctcggg ctgccacccg
     1801 gggcggaccc tgatggcgtc tccatggtgt atctggtgag cgccttcccc ggcgcggagc
     1861 gcgcccgcgc cgattcgctc agtaagcaag ttcgcgagct actgcctcgc gcctgcgtag
     1921 tgagggtttt ttgtcccgga gtcaccgctc tgtccgagtc gggcaagggc gccggccata
     1981 ccgaaccgac cgtgaattgc ctcgggcagg cgatcgaaat ctgcgtgtcc tggcaggagg
     2041 tgcgtagcac acgagacccg gcgccggatc ttacatcgct gatggcgcgc gaacggcgta
     2101 gtgcattaca gcattctgag ggacttcgtc gcaggcgcga gcataccgag gaggtactcc
     2161 agtaacccag cagtgagttc caagtgccag tcttctcctt gatatgcgcg acccgagctt
     2221 cggtcgacag cgcccgttgc ttgc
//
LOCUS       DATMGX010000789         2693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_79775, whole genome shotgun sequence.
ACCESSION   DATMGX010000789 DATMGX010000000
VERSION     DATMGX010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79775"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1047)
                     /locus_tag="VNR65_12920"
     CDS             complement(175..1047)
                     /locus_tag="VNR65_12920"
                     /inference="COORDINATES: protein motif:HMM:NF020478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HWJ19619.1"
                     /translation="MKHRLLPQAALSILAASITLFPEPGASHAQLAPAAKAAQLAAHR
                     AVYDLTLDKTGAGSNVSDIRGQLTYDFSGSECQGYTLNTRLITEIFDREGKPSTTDIR
                     SESQEDAESRHFRFNTSQYVNDKLSESTKGLAVRPSRSREIVNVELEKPKRGMLTLSG
                     NIYFPTQHSLAILKAAISGENRLQADIYDGSEKGTKVYETTTVIGAPLELAANAQLPP
                     IKNSEALDVIQSWPVVVSYYDQSPKKDGLPAYEVSFRMYANGVSRKLKLDYGTFSLNG
                     ELSAIEFSKTKPCP"
     gene            complement(1128..>2693)
                     /locus_tag="VNR65_12925"
     CDS             complement(1128..>2693)
                     /locus_tag="VNR65_12925"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ19620.1"
                     /translation="GSQQLMQPFAGTLARNGFIAVTFDCLGHGHNPQPMTGDVTKVEE
                     GPTPALVREFGKVAAFAQGLPGNDGRIAVLGHSMASDIVIRYAQAHPEVAATVAVSMF
                     SPVVTPTSPRNLAVIVGDNEPGKLKDEGRRVVSMVSGGTPDIGVTYGSFADGTARRVS
                     FSPGVEHIGVLYSPESMRQARDWLDTAFGRNGNGYLDERGPALGLLFFGLTLLGWPLA
                     GLLPRVSVPPTGAGLRWRRLLPVAIAPAILTPLILWKMPTNFLPILLGDYITMHFALY
                     GLLTLAGLWLISRGGAPKQAKASPRPDYAVATWADKQPPKEATLIAPPPSNLMLPGPT
                     TGVRTNEIVAINQRDRSLTLTTGASAPPPPPVQNARSTKKYLVAKYIVATLLVAAYSI
                     FAFGVPLDRYVLSFSPIPERVPLILAVLAGTLPFFLADEWLTRGQGAARGSYPVTKLL
                     FLLSLALAVALNFKKLFFLILIIPIILIFFIIYGLFSGWVNRRTGNPLVGGAAIALAF
                     AWAIAVTFPLLRL"
BASE COUNT          549 a          759 c          842 g          543 t
ORIGIN      
        1 gatcgagggc gtgatcaagc gcatcgaggg ggtatgattg ggggtatttt ttgcgcaacg
       61 ggataagatc cctcatgtat tgcagaaggt tacagttttg attcgaatcc tggcacccca
      121 gccattccct tccgttacca gctgtttgcc gaagagttct tgatcaccgc tgtctcaagg
      181 gcaaggcttc gtcttcgaaa actcgatcgc cgacaactcc ccgttgagtg agaatgtgcc
      241 gtaatcgagt ttcagcttcc ggctgacgcc attcgcgtac atgcgaaaag acacttcgta
      301 agccgggagg ccgtccttct taggactctg gtcgtaatag ctcaccacga ccggccagga
      361 ctggatcaca tccagcgctt ccgagttctt gatgggcggc aattgcgcat tcgccgcaag
      421 ttccagcggc gcgccgatga ccgtcgtcgt ctcgtagacc ttcgtgcctt tctccgatcc
      481 gtcgtaaata tcggcctgaa gacggttttc gccggagatc gcagctttca ggatcgcgag
      541 tgaatgctgc gtgggaaaat agatattccc ggacaaggtc agcattcccc ttttgggttt
      601 ttccagctcc acattcacga tttcgcgcga ccggctcggg cggaccgcga ggcccttcgt
      661 gctttcgctc agcttgtcgt tgacatattg agaggtgttg aaccggaaat gcctgctttc
      721 cgcatcctcc tggctttcgg accggatgtc ggttgtgctt ggttttcctt cgcgatcaaa
      781 gatttccgta atgagacgcg tgttgagcgt atagccctgg cactccgatc cgctgaagtc
      841 gtaggtgagc tgtccacgaa tgtcgctgac attggacccc gcacccgttt tgtcgagcgt
      901 gaggtcgtaa accgcccggt gcgcggcgag ttgggcggcc tttgccgcag gggcaagctg
      961 agcgtgagaa gctccaggct cggggaagag ggttatgctc gcagcaagaa ttgataatgc
     1021 cgcttggggc agtaaacgat gcttcattga agtgtccgct gttctaaagc ttattgataa
     1081 gcgatttccc ggaaaaaaac agacgtttga gcgtccttgt tgcgccatca cagcctgagc
     1141 agcggaaagg tgacggcgat ggcccaggcg aaggcaagtg caatcgcggc cccgccaaca
     1201 agagggttcc ccgtccgccg attgacccaa ccgctgaaca agccgtagat gatgaagaag
     1261 atcaggatga tcgggatgat caggatcaga aagaacagct tcttgaagtt cagcgcaaca
     1321 gccagcgcca gtgagagaag gaagagaagt ttcgtcacag gatagctgcc tcgcgccgcc
     1381 ccttgccctc gggtgagcca ttcgtccgcg agaaagaatg gcagggtccc ggcaagcaca
     1441 gcgaggatga gcggaacacg ctcggggatc ggtgagaagg acagcacgta ccggtcgaga
     1501 ggcacgccga aggcgaagat gctgtaggcc gccaccagaa gcgttgcgac aatgtatttt
     1561 gcgaccaagt atttcttggt gctccgcgcg ttttgaaccg gcggaggggg cggggccgag
     1621 gcgcctgttg tcagggtgag gctgcgatca cgctgattta tggcgacgat ttcattcgtt
     1681 ctgactccag tcgtgggccc aggcagcatg agattggacg gaggcggagc aataagcgtc
     1741 gcctctttgg ggggctgttt gtctgcccac gtggcgacgg catagtccgg ccgtggcgaa
     1801 gcttttgcct gtttgggagc accgccgcgc gagataagcc aaagcccagc cagcgtaaga
     1861 agcccataca aagcgaaatg catggttatg tagtcgccga gcaggatggg aagaaagttc
     1921 gtcggcattt tccataggat gagcggcgtc aggatcgcgg gcgcgatcgc gacgggcaga
     1981 agcctgcgcc acctaagccc cgcccccgtt ggaggaaccg acacgcgcgg aagcagcccc
     2041 gccagaggcc agcccagaag cgtcagcccg aagaacagga ggccaagcgc cggaccgcgt
     2101 tcgtcgagat agccattccc gttgcgcccg aacgccgtgt cgagccagtc gcgagcctgc
     2161 ctcatgcttt cggggctgta gaggactccg atatgctcga cgccgggcga gaaagaaacg
     2221 cggcgcgcag ttccgtcggc gaagctgccg taggttacgc cgatgtcggg cgttccaccg
     2281 gacaccatgc tcaccacacg ccgcccctcg tccttgagct tgcccggctc gttgtcgcca
     2341 acgatgaccg cgagattgcg cgggctggta ggggtgacga ccggcgaaaa catcgagacg
     2401 gccaccgttg ccgccacttc ggggtgcgcc tgcgcatagc ggatcacaat gtcgctcgcc
     2461 atggaatgac cgagcacggc gatacgcccg tcgttgcccg ggaggccctg cgcgaatgcc
     2521 gcgaccttcc cgaattccct gacgagcgcg ggagtggggc cttcctctac cttcgtcaca
     2581 tcgcctgtca tgggctgcgg attgtgtcca tggccgaggc agtcgaatgt cacggcgata
     2641 aagccgtttc gcgcgagtgt cccggcaaag ggctgcatga gttgctgcga gcc
//
LOCUS       DATMGX010000790         2476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_345880, whole genome shotgun sequence.
ACCESSION   DATMGX010000790 DATMGX010000000
VERSION     DATMGX010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345880"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VNR65_12930"
     CDS             complement(<1..513)
                     /locus_tag="VNR65_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HWJ19621.1"
                     /translation="MAAKDVRFAQDARERMLRGVDILADAVKVTLGPKGRNVVLDKSF
                     GAPRITKDGVTVAKEIELEDKFENMGAQMVREVASKTNDIAGDGTTTATVLAQAIVRE
                     GMKSVAAGANPMDLKRGIDLAVAAVVKDLESKAKKVTSSAEVAQVGTISANGDTEVGA
                     KIAEAMEKVGN"
     gene            complement(562..882)
                     /gene="groES"
                     /locus_tag="VNR65_12935"
     CDS             complement(562..882)
                     /gene="groES"
                     /locus_tag="VNR65_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWJ19622.1"
                     /translation="MKFRPLHDRVVVRRLEEEERTKGGIIIPDTAKEKPQQGEVIAVG
                     PGARNEQGQLVALDVKEGDRILFGKWSGTEVKIDGEDLLIMKESDILGVLENHEGKSK
                     KKAA"
     gene            1147..1947
                     /locus_tag="VNR65_12940"
     CDS             1147..1947
                     /locus_tag="VNR65_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19623.1"
                     /translation="MSNTLSSASSLEDRKQPEKRKRRGVAWWWLLVFLWMGALVYVPI
                     WYASLYDKKPAPVAAAPVPAPVVAAPAPAVEAPVKKHAEKQKVENAFFFRVDGKDSRG
                     QPATFDFIMLTNEFTWAKGSTTQVISGDKVIPEAEAADRVLTQQVRDSLASASDLIAV
                     GLASSEGERTQEEARALDRAKTVSHWMSKVSKPEMALWTLTLGQYDKACKEQEDADSS
                     FERPVIFAGVRAKADGANLQEALADAISGHDNLPSRGCYSRFDLDKIR"
     gene            2263..>2476
                     /locus_tag="VNR65_12945"
     CDS             2263..>2476
                     /locus_tag="VNR65_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19624.1"
                     /translation="MSDSPASDPNKKQPGDEKRRIEIPAWWSIVALIMTCLLIVPIHY
                     ATTHTKKNVPAEAEAKPNAAETASPAK"
BASE COUNT          512 a          710 c          699 g          555 t
ORIGIN      
        1 gttaccgacc ttttccatgg cctcggcgat cttcgcgccg acttctgtgt cgccattcgc
       61 ggagatcgtg ccgacctggg cgacctcagc tgaggacgtc accttcttgg ccttgctttc
      121 gagatccttc accacggcgg caacggcgag gtcgataccg cgcttcagat ccatcgggtt
      181 cgcgccagcg gcaacggact tcatgccttc gcgaacgatc gcctgggcga gaacggttgc
      241 ggtggtcgta ccatcgccag cgatgtcgtt cgtcttggaa gcgacttcgc gcaccatctg
      301 cgcgcccatg ttctcaaact tgtcctcaag ctcgatctcc ttggcgactg tcacgccgtc
      361 cttcgtgatg cgcggggcgc caaaggactt gtcgagcacg acgttgcggc ctttcgggcc
      421 aagcgtcacc ttcacggcat cggcaaggat gtcgacgcca cgcagcatcc gctcgcgggc
      481 atcctgggca aaacgtacgt ctttagcagc caattctatg actcctcgaa taagttgaat
      541 ttgttcagag attagagaat gttacgcggc ttttttcttg ctcttgcctt cgtggttctc
      601 aagaaccccg aggatgtcgc tttccttcat gatcagaagg tcttcgccgt cgatcttgac
      661 ttcggtgccg gaccatttgc cgaagagaat gcggtcgcct tccttcacat cgagagcgac
      721 cagctggccc tgctcattgc gagcgccggg gccgactgcg ataacttcgc cttgctgagg
      781 cttctccttg gccgtatccg gaatgatgat cccgcccttc gtgcgctctt cctcttcgag
      841 acggcgcact accacgcggt catgcagcgg cctgaatttc atggcatcct cctaagacga
      901 taaatagatc ggttggaacg ggtgcggcgt ttacgctcgc gtggccctgg gctatgagat
      961 atttagaact gttagcactg tcaaggggcg agtgctaatg tctcctctgg ttttcacatc
     1021 ctaaagcgct tcagtaaatt ttgaccaaac gcggtcatat tcgtgtcgcg gtgccgtggc
     1081 agacggcgca aaggggagca ttttcgctgc caagccatac cggaatttca taggagagca
     1141 tttctcatgt cgaatactct ttcttcggcg tcgtcgctgg aagataggaa gcagcctgaa
     1201 aaaaggaagc ggcgcggcgt cgcttggtgg tggctgctcg tctttctttg gatgggagcc
     1261 ctcgtttacg tcccgatctg gtacgcaagc ttgtatgaca aaaagcccgc tccggtagcc
     1321 gctgcgcccg ttccagctcc ggtagtcgct gccccggccc ccgcggtcga agcgccggtg
     1381 aaaaagcatg ccgagaagca gaaggtcgaa aatgcgttct tcttccgcgt cgacggcaag
     1441 gacagccggg gacagcccgc cacgttcgat ttcatcatgc tcacgaacga attcacctgg
     1501 gccaagggaa gcacgacaca agtgatttcc ggcgacaaag tcattccgga ggccgaagca
     1561 gctgatcgcg ttcttactca gcaagtgcgc gactcccttg cgagcgcaag cgatttgatc
     1621 gcggtcgggc ttgcatcctc cgagggcgag cgcacccagg aggaggcgcg tgcgctcgac
     1681 cgcgcaaaaa cggtctcgca ttggatgtcg aaggtcagca agcccgagat ggcactctgg
     1741 accctgacgc tcggccagta tgacaaggct tgcaaagagc aggaggatgc agactcgagc
     1801 ttcgagcgcc ctgttatttt tgctggcgtc cgcgccaagg ccgatggtgc gaatttgcag
     1861 gaagctttgg cagacgccat cagcggtcac gataatctgc cgagccgcgg ttgctattcc
     1921 cgctttgact tggacaaaat ccggtaagag ctggttctgc gcctgaattc gtcaaatgga
     1981 agaagcacga gccgctcagt ctgttgggcg gctcttgttc gttatgcact gctcgcaggt
     2041 tcgcaaccat cgctggtgat gaagattgcc gcgtttttgt tcgttcacct gctaataaaa
     2101 taggcggaac tgtcagccgg acgaatgctc aatgcttgca gcgtcaaata gagctgtcgc
     2161 aaaacctggc aaaacatgag cataatcatg tctcagccgt ggcgaggcgc tcacaaagca
     2221 ctgtgctaat gctatcgccg ttttcatagc ggagcgttca taatgtcaga ttcccctgca
     2281 tccgatccaa acaagaagca gcccggcgat gagaagaggc gcatagagat acctgcatgg
     2341 tggtctattg tcgcactcat catgacctgc ctgcttattg tgcctatcca ttacgcaacg
     2401 acacacacga agaaaaacgt accggcggag gcagaggcaa agccgaatgc cgccgagaca
     2461 gcgtctccgg ccaagg
//
LOCUS       DATMGX010000791         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_429522, whole genome shotgun sequence.
ACCESSION   DATMGX010000791 DATMGX010000000
VERSION     DATMGX010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429522"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..299
                     /locus_tag="VNR65_12950"
     CDS             114..299
                     /locus_tag="VNR65_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19625.1"
                     /translation="MAKAKVTFKTVRNSADDWNIVAEYPGVEPREITGLKSKADADDW
                     MNGDRRVAWLRSQGYAK"
     gene            671..1120
                     /locus_tag="VNR65_12955"
     CDS             671..1120
                     /locus_tag="VNR65_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ19626.1"
                     /translation="MRYLHTMLRVRNLDAALKFYQDALGLKEVRRVDNEKAKFTLVFL
                     CAPEDEGILKASKGRGAPLVELTYNWDEEKYGEDRYFGHLAYEVDDIYATCDRLMKMG
                     ITINRPPRDGQMAFVRSPDLHSIELLQKGDAKPPAEPWTSMPNTGHW"
     gene            1257..1427
                     /locus_tag="VNR65_12960"
     CDS             1257..1427
                     /locus_tag="VNR65_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19627.1"
                     /translation="MSQSQHYKFQAEQAKRLARQVTDSAVRERLLEMAGEYGRYAELM
                     EARERPREPAFG"
     gene            1766..2413
                     /locus_tag="VNR65_12965"
     CDS             1766..2413
                     /locus_tag="VNR65_12965"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWJ19628.1"
                     /translation="MPTDNQGQLTIWGRANSVNVQKVLWCLRELDLAFRRIDAGMAFG
                     RNHDPDYLAMNPNARVPTLVDGDFVLWESNSIMRYLVLAYGKGSPVYPEAPKRRAGVD
                     RWLDWTLSTVQPVDRPVFWALVRTPAEKRDMAAIQRDADAEAEVWRVAEAQLKSRRFI
                     EGDDFTIADIALGAYARRWFGVEGISKPKLPHLERWFAQFAERPGFAQFIAPPMS"
BASE COUNT          503 a          747 c          727 g          487 t
ORIGIN      
        1 caagaccagt tgagccagga cctcggccat tctccttata tcggaagcga aattctaatt
       61 tccagttccc aagtgccgct tctctttttg tcgaattacc tgcataacag cgcatggcaa
      121 aagcaaaagt aaccttcaag accgtccgaa actctgcgga cgactggaat atcgtggctg
      181 aatatccggg cgttgagccc cgagaaatca ccggcttgaa aagcaaagcc gatgctgatg
      241 actggatgaa cggcgaccgc cgcgtagctt ggctgcgctc tcagggctac gctaagtgac
      301 ctgataactt tccatcgtat cgccttcagc ttcgcccagg cggcggcggc attgtcccta
      361 ctccagacag acgccgtccc cgtcctggca ctttggcgga cggcttgcgc ggggcgctga
      421 aacgagagcc actctctgcc tcgaccgaaa gctctttctg tcgggttgag acccgttcag
      481 ccaacggaaa gggattcgat ttgcatgatg gcctcaggtg gccacaagca ctcacgcgaa
      541 cgcaaagggc acggactggc acgcaaacgg cacgcagccc cctgcccttt ggcgcatttt
      601 gctgctatct gatacccgaa ccgcccaaac caaaaccaaa tctccaaacg ttctgaacgg
      661 agtaagaaac atgcgttatc tccacaccat gctgcgcgtg cgcaatctcg atgccgcgct
      721 gaaattctat caggacgcgt tgggattgaa ggaagtccgc cgggtcgaca acgaaaaggc
      781 caagttcacg ctggtgttcc tgtgtgcgcc cgaggacgaa ggcatcctga aggcgtccaa
      841 ggggcgcggc gcgccgctgg tcgagctcac ctacaattgg gatgaggaga aatacggtga
      901 ggaccgctat ttcggccacc tcgcctacga agtcgacgac atctacgcca cctgcgaccg
      961 gctgatgaag atgggtatca ccatcaaccg tccgccgcgc gacggccaga tggccttcgt
     1021 gcgctcgccc gacctgcact cgatcgagct cctgcaaaag ggcgacgcca agccgccggc
     1081 ggagccatgg acctcgatgc cgaacaccgg ccactggtaa cgcccttcga agccggcgtg
     1141 cacgaattcc ccgtgcatcg ccggcatccc ggcggtgtgc cagcagcgcc cctgattctc
     1201 gttcacgatc ggtgaacctt ttgccctcag actcgacgta gattctgagg tgacccatga
     1261 gccaaagcca gcactataag ttccaggccg agcaagccaa acgtttggcc cgccaggtca
     1321 cggactcagc agtgcgcgag aggctcctgg agatggctgg agaatacggc cgctacgccg
     1381 aactgatgga agcccgcgaa cgaccgcgcg aaccggcctt tggctgattt tggttccagt
     1441 attccggaaa agttttctgt tcccgtcgca ggaactccac gttgggcttc gcgttcttct
     1501 ctcgattgag gaattcgaag gaggcgaaca tgggacgagg aatactgctc tggctactgg
     1561 gcgtgccgat accggtaatc attctgctgt ggctgttctt cggccgctga catcaggcaa
     1621 tccgcacatt ctcgcgcaaa ttagggttcg ccattggcgg gccttttttg cggagcggca
     1681 ttgttgaaat cgacaggtgg cacgggcttt ttcgcgcgtt ttccgatatg acccgctcaa
     1741 agcggatcac cgggagcgaa atatcgtgcc gaccgacaat cagggacagt tgaccatctg
     1801 gggccgggcc aattcggtca atgtccagaa agtgctttgg tgcctgcgcg aactcgatct
     1861 cgcttttcgg cgcatcgacg ccggcatggc ctttggcaga aatcacgatc cggattacct
     1921 cgcgatgaat cccaacgcgc gggtgccgac gctcgtggat ggcgattttg tcctttggga
     1981 atccaattcc atcatgcgtt atctcgtgct cgcctacggc aagggatcgc ctgtctatcc
     2041 cgaggcgccg aagcggcgcg ccggcgtcga tcgctggctg gactggacgc tgtcgactgt
     2101 acagccggtc gatcggccgg tgttttgggc cctggtgcga acccctgcgg aaaagcgcga
     2161 catggccgcg atccagaggg acgcggacgc cgaagccgag gtatggcggg tagccgaggc
     2221 gcagcttaaa tcgcggcgct tcatcgaagg cgatgacttt acgattgccg atatcgcgct
     2281 cggcgcctat gcgcggcgct ggttcggcgt cgaaggcatc agcaagccga aactccccca
     2341 tctggaacgc tggttcgcgc aattcgcgga gcggccggga tttgcccaat tcatcgcgcc
     2401 gccgatgtcg tgaggcgcag atccgcggtt cgccgctcag cgcgcgccgc cggaggtgcc
     2461 ggcg
//
LOCUS       DATMGX010000792         3397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_389263, whole genome shotgun sequence.
ACCESSION   DATMGX010000792 DATMGX010000000
VERSION     DATMGX010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389263"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VNR65_12970"
     CDS             complement(<1..640)
                     /locus_tag="VNR65_12970"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWJ19629.1"
                     /translation="MTILGWVQIALYALVIILIAKPLGWYMTRVFSGQRVPLYWPLRP
                     IERALYWVSGVNERQEQSWITYAVSMLFFSVAGFVTLYALQRFQASLPFNPQGQAAVT
                     PDLAFNTSVSFITNTNWQSYTPETTMSYLVQMAGLTVHNFLSAATGVALALALIRGFS
                     RRTSNTVGNFWVDMTRCTLYVLLPVSIVVGLFFVWQGMPQNLNPYTEVATLEG"
     gene            complement(653..742)
                     /gene="kdpF"
                     /locus_tag="VNR65_12975"
     CDS             complement(653..742)
                     /gene="kdpF"
                     /locus_tag="VNR65_12975"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HWJ19630.1"
                     /translation="MSFDYILAGAVTALLLGYLVYALVRPERF"
     gene            complement(1123..2178)
                     /locus_tag="VNR65_12980"
     CDS             complement(1123..2178)
                     /locus_tag="VNR65_12980"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWJ19631.1"
                     /translation="MSIQLGHETGKSKASPNWLQRMAANLGFTNGVSLRETLETAIQE
                     MGAEDDQSLSQQERLMLLNVLEFGETKVSEVMIPRADIVAVEEHQTVAELLNLFARIG
                     HSRVPVFRDSLDNPVGMVHIKDAIAWVTRGAKLSLQDETVVERAVQSLDLSRSALKTT
                     IASAKLVREALFVPSSMSALALLAKMKQKQIHLAIVVDEFGGTDGLATFQDVVEAIIG
                     DIADEHDDSKALAIRVENEAFIASARTPIEDVEQALGMSLTTNGIPEEVETLGGLIMA
                     TVGSVPKRGQIIPHPSGVTFEILEAGPRRLHKVRIFKQLALAAPDKPLLLAAPEGGPA
                     PESIERDGATLRDARAA"
     gene            complement(2206..2703)
                     /gene="ybeY"
                     /locus_tag="VNR65_12985"
     CDS             complement(2206..2703)
                     /gene="ybeY"
                     /locus_tag="VNR65_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWJ19632.1"
                     /translation="MDIVIEDTRWGSVTGLEDLIPNLAAETLRAVQLSTGSHSVSVAL
                     LSDAEVRGLNKAFRGKDAPTNVLSFPSPAAMHIGGRNGEPLFLGDVALAYETVVSEAS
                     AQGKTVLQHAAHLVVHGVLHLAGFDHDGDGDAERMEAAEGAILARFGIPDPYRDDAAS
                     PAAAL"
     gene            complement(2809..>3397)
                     /locus_tag="VNR65_12990"
     CDS             complement(2809..>3397)
                     /locus_tag="VNR65_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWJ19633.1"
                     /translation="RALQSRELVFGTGPAGTGKTYLAVAYAAGCIERGEVDRLGLSRP
                     AVEAGERLGFLPGDMKEKVDPYLRPLYDALYDVLPPERVERGLETKMIEIAPLAFMRG
                     RTLAHSIVILDEAQNCTAMQMKMFLTRLGEGSKMIITGDPSQVDLPMGQSSGLTDALS
                     ILADVEGIAHIAFTRADVIRHPLVGRIIEAYETKR"
BASE COUNT          714 a         1015 c         1003 g          665 t
ORIGIN      
        1 cgccttcgag agtggcaacc tcggtgtagg ggttgaggtt ttgtggcatg ccctgccaca
       61 cgaagaagag gcctaccacg atggagacgg gcagcagcac gtaaagcgtg cagcgggtca
      121 tatcgaccca gaaattgccg accgtgttgg atgtgcgccg cgagaaaccg cggatcagcg
      181 ccagtgcaag cgcgacgccg gttgcggcgg agaggaagtt gtgcaccgtg agccccgcca
      241 tctgcacgag atagctcatg gtcgtttccg gcgtgtagga ctgccagttc gtgttggtaa
      301 tgaagctgac agaagtgttg aaggcgaggt cgggtgtgac cgcggcctgc ccttgcgggt
      361 tgaacggcag cgaagcctgg aacctctgca aagcatagag cgtcacaaac ccagccacgc
      421 tgaagaacag catggacaca gcgtaagtga tccagctctg ctcctgcctt tcgttgacgc
      481 cgctaaccca gtacagcgcc cgctcgatcg gccgaagcgg ccagtaaagc ggaacccgct
      541 ggccgctgaa cacgcgggtc atataccagc ccagcggctt cgcgatgaga atgatcacga
      601 gcgcatagag tgcaatctgc acccagccta gaattgtcat ggatgacgct cctcagaacc
      661 gctcaggacg gacaagcgcg tagacgaggt agccgagcaa aagcgcggtg acagcccccg
      721 ccagaatgta atcgaaagac ataaggtcct cacaatcggt cgcaggccgc gacataggcg
      781 atggcgataa cgaagaagcc gactcccatg gccagcatca caatgtcagt catttcgagc
      841 ctcagatttc tgccgcacgc gaggcccggg agggaggcac tcggtgcggc tatcgcggca
      901 aatcggcaat cagacatata aaaacaattc gagcgctcac gcgctcctat aggaatttca
      961 tagtcgtgtg atgagcgcgt cccttaccgt cacgccggaa tggctgcgcc atatccgaga
     1021 tcgtcagaat ccccgtctgg ggggatgtaa ggttcccgga taacgcctgc ggcgtttccg
     1081 gatgacgaag gtggaggctg aatggctcgg taaagctccg ccttaggcgg ctcttgcatc
     1141 ccggagagtc gcgccatccc tctcgataga ctctggcgcc ggtccaccct cgggggccgc
     1201 gagcagtagc ggcttatcag gggccgccag ggcgagttgc ttgaaaatgc gaaccttgtg
     1261 caagcgcctc ggccctgctt ccaggatctc gaaggtcacg ccggacggat gcggaatgat
     1321 ctggccgcgc ttcgggacac ttcccaccgt ggccatgatg aggccgccca aggtctccac
     1381 ttcctccggg atcccgtttg tggtcaggct catgccgagc gcctgctcga cgtcctcgat
     1441 gggcgtgcgt gcgctcgcta tgaacgcctc gttctcgaca cggattgcga gagctttgct
     1501 atcgtcgtgc tcatcggcga tgtcgccaat gatcgcctcc accacgtcct ggaaggtggc
     1561 gaggccatcc gtccccccga actcgtccac gacgatggcg aggtggatct gcttctgctt
     1621 catcttcgcc agcaacgcca aggcggacat cgaggatggt acgaagagcg cttctcgaac
     1681 aagcttggct gaggcaatgg tcgtcttcaa agccgaccgg ctcaagtcga ggctttgaac
     1741 agccctttcc acgacggtct catcttgcaa gctcaatttc gcgcctcgcg tgacccaggc
     1801 gatcgcatcc ttgatgtgaa ccataccgac cggattgtcg aggctgtcgc ggaagacggg
     1861 cacgcgcgaa tggccgatgc gcgcaaagag gttcaggagc tccgcgacag tttggtgctc
     1921 ctccacggcg acgatgtcgg cgcgcgggat catcacttct gagactttgg tttcgccaaa
     1981 ttcgaggaca ttgagcagca tcaggcgttc ctgctgcgac agagactggt cgtcctccgc
     2041 acccatctcc tggattgcgg tttcgagcgt ttcccgcagg ctgacgccgt tggtgaaccc
     2101 gaggttggcc gccatgcgct gaagccagtt tggcgatgcc ttcgatttcc ccgtttcgtg
     2161 cccgagttgt atagacatag cttttgtgtc gttaccgtgt tgtgattaaa gagcagctgc
     2221 aggcgaagca gcgtcgtccc ggtagggatc gggaatgccg aagcgggcga gaatagcgcc
     2281 ctcggcagct tccatgcgct cggcatcgcc gtcgccgtca tggtcgaacc ctgcgagatg
     2341 caagacgcca tgcacgacga ggtgagccgc gtgctgaagg acagttttcc cctgggccga
     2401 agcttcgctc acgaccgtct cataggccaa agccacgtct ccaaggaaaa gaggctcgcc
     2461 attgcggccc ccgatatgca ttgcggccgg cgagggaaat gacagaacat ttgtcggggc
     2521 atccttgccc cggaaggcct tgttcagccc gcgcacttca gcgtcggaaa gcagcgcaac
     2581 gctgacgctg tggcttccag ttgaaagctg caccgcacga agagtttcag ccgcaagatt
     2641 cggaataagg tcttccagcc ccgtcacgga tccccagcgt gtgtcttcaa tgacaatatc
     2701 catgcacgtt cgggatggct cgccgtcacc gctcgaaccg tctttctccg aagacgccgc
     2761 agactcaata gttgtatcca taccgcttgg tatcataata gtcgtaactt agcgctttgt
     2821 ttcgtaagct tcaataatac ggcccacaag tggatgacga atcacatccg cgcgggtgaa
     2881 ggcaatatgg gcgatcccct cgacatcggc gaggatcgaa agcgcgtctg tgaggcccga
     2941 tgattggccc atcggcaggt ctacctgcga agggtcaccg gtaataatca tcttgctgcc
     3001 ttctccaagg cgggtcagaa acatcttcat ctgcatcgca gtgcaattct gcgcttcatc
     3061 gagaatcacg atcgaatgag ccagcgtccg gccacgcata aacgcgagcg gtgcgatttc
     3121 gatcatcttc gtctcgagtc cacgctccac cctttccggg ggaagaacat catacagggc
     3181 gtcatagagc ggacgcaaat aaggatcgac tttttccttc atatccccag gaagaaagcc
     3241 aagacgttca cccgcttcga ctgcaggccg cgataagccc agccgatcca cctcgcccct
     3301 ttcgatgcac ccagccgcgt aggcgacggc aaggtaggtc ttgcccgtgc cggccggccc
     3361 ggttccaaag accagttcgc ggctttggag cgcccgg
//
LOCUS       DATMGX010000793         5127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_429612, whole genome shotgun sequence.
ACCESSION   DATMGX010000793 DATMGX010000000
VERSION     DATMGX010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429612"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VNR65_12995"
     CDS             <1..456
                     /locus_tag="VNR65_12995"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HWJ19634.1"
                     /translation="APSYLARHGEPSHWEELALARCLNYGHTTTLQRWQLTQNGETIA
                     VPINSALCSNNGDVLRAAALAGRGITKLPTFLVGPDIKAGRLSIVLAEYPPTDLTIYA
                     LYTPNRYLAAKTRVFIDFLTRRFGGEPEWDSHRMPGAGKAAFARGEPLR"
     gene            739..1137
                     /locus_tag="VNR65_13000"
     CDS             739..1137
                     /locus_tag="VNR65_13000"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HWJ19635.1"
                     /translation="MGNLIAFGFDRSWAASEVLKKLRTDTAVDEAYVVERSASGRCDI
                     RWASNQIAPEAADPSSEGLWGALVRLIYLNSSLDLTIPRGSSALFVHLTNAAERRVLR
                     EVKPYRARILETSLSREAEQRLKAEFTKAA"
     gene            complement(1166..2035)
                     /locus_tag="VNR65_13005"
     CDS             complement(1166..2035)
                     /locus_tag="VNR65_13005"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWJ19636.1"
                     /translation="MSDSHNTIAPQICILGYGRLAEALADRLSQVCSRVSVYARRSPP
                     EGTNKVHFFNDVTQAINGARFVLAVTSDFASIVAILQDIDRLDGKTFIQISTLAPSQS
                     LAVEREVTGRGGRYVENPVLGSRPEARSGTLIAMAGPAANIDDEVSAFIDLYSNRRIV
                     FDAVGQASALKLIFNFLIGTLTASFSVALNAAQENRIDVDQFMGLLRESALYAPTFDK
                     KLNQMLTGQYTNPNFPAEHLKKDLNLFGEVAHLSPLATAFVSALSDIAAAAADRQETK
                     LDYSSLHEVVKGR"
     gene            2922..3329
                     /locus_tag="VNR65_13010"
     CDS             2922..3329
                     /locus_tag="VNR65_13010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase-related protein"
                     /protein_id="HWJ19637.1"
                     /translation="MTADTFDLITRYYAAFNAGDMETFLSMLSDDVVHDINQGEREAG
                     KVAFAAFMERMNLHYREKLVDITVMVAADGTRAAAEFTVLGEYLSTDEGLPPAEGQTY
                     RLNAGAFFVVRDGKVARVTNYYNLQDWLRQVGG"
     gene            complement(3356..4003)
                     /locus_tag="VNR65_13015"
     CDS             complement(3356..4003)
                     /locus_tag="VNR65_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HWJ19638.1"
                     /translation="MESRTPVGTPTLNLEMLLDILRATAQEGLSYAENDYDRARYQKL
                     LNLASEKYASITGLYPEAIREMFLRDHGSITPKVGVDIAVPNHNGEILVLQLPNGKWC
                     LPGGWMDVGESPFDTAQRETREETGLQITPLGIITVGHRSPLNHPGAISQINICVGAQ
                     SLPNDAEVTVSHEHSAYRWISDVNEVEDWRRGHKRFFPLILRAYRDQAFFPIVND"
     gene            4402..>5127
                     /gene="gluQRS"
                     /locus_tag="VNR65_13020"
     CDS             4402..>5127
                     /gene="gluQRS"
                     /locus_tag="VNR65_13020"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HWJ19639.1"
                     /translation="MPLTTRFAPSPTGYLHLGHAYSAHFAYEAARTEAGTFLLRVEDI
                     DPIRCRPEFAQALFEDLRWLGLTWEEPVQRQSEHMEDYAAALAKLRTMGLLYPCFCTR
                     REIEHEVMAAAAAPHLTPQGPDGWVYPGTCRNLDPGERARRLLQNEGANWRLDVAKAF
                     AITGPLYWSDRAAGRVEAAPLDFGDVVLARKDVPASYHLNVTVDDHLQHVTLVTRGKD
                     LFRATDIHVLLQAILGYATPDYHH"
BASE COUNT         1102 a         1427 c         1462 g         1136 t
ORIGIN      
        1 gcgccatcct atcttgccag gcatggggag ccctcgcact gggaggaact cgcgctcgcc
       61 cgctgcctca actacggcca tacgaccacg ctgcaacgct ggcagttgac gcaaaatgga
      121 gagacgattg ccgtgccgat caactcggcg ctatgctcga ataatggcga tgtgctgagg
      181 gcggctgcac tggcgggccg cggcatcact aagctgccca catttctcgt cggcccggac
      241 atcaaagccg ggcgcttaag catcgttctt gcggaatatc cgccaacgga cctcaccatc
      301 tacgcgcttt acacgccaaa ccgctatctt gccgcgaaga cgcgcgtttt catcgacttc
      361 ctgactcgcc gctttggtgg cgaacccgag tgggactcgc atcgaatgcc cggagcggga
      421 aaggccgcct tcgcacgagg cgagccactg cgctgaaggc gcacatcgcc gtgcgtcgaa
      481 accattggct cgctttgaga tcccactccc cccaaaaggg tttcgctata tatttcaagt
      541 tgatagcggg tcgccaccaa aacgccccaa agtcattcaa tgaatctcga acatcctgaa
      601 aaccctggaa taaattcttg agcttaaccg cgcaaatatt ctagcttttg aattggcatt
      661 tgctttctgt atcaacttat ttgatcagga caaccgggta tattcacctc aacacacaag
      721 atgaaagtga gatgagctgt gggaaatctt attgcatttg gattcgatcg ttcctgggcc
      781 gcgagtgagg tcttaaaaaa actgcggacg gacactgccg ttgatgaagc ctacgtggtt
      841 gagcggtctg cctccgggcg atgcgacata aggtgggctt ccaatcagat cgcacccgag
      901 gcggccgatc cctcgagcga agggttatgg ggtgcgctgg taaggcttat ctacctcaat
      961 tcgagccttg atctaaccat cccgcgcggg agctcggcgt tgtttgttca cctcacgaat
     1021 gcggcggaga gaagggtcct cagggaggtc aaaccgtata gggcgcgcat ccttgaaacc
     1081 tcgctctcgc gggaggctga acagaggctc aaagccgaat ttacaaaggc ggcatgatcc
     1141 tgtcccgccg ctcttggcct ttcggtcatc ggccttttac aacctcatgc agcgaggaat
     1201 aatcgagttt ggtctcctgc ctgtcggctg ctgctgcggc gatgtcggag agcgcactta
     1261 caaacgccgt tgccaggggc gacaggtgcg cgacttcgcc gaagaggttc aggtctttct
     1321 tcaaatgttc tgcgggaaag ttcggattgg tatattgccc cgtcagcatt tgattgagct
     1381 tcttatcgaa ggtcggcgca taaagcgcgc tttcgcgcag gagacccatg aactgatcga
     1441 catcgatgcg gttttcctgg gcggcgttaa gagccacgga gaaggatgcc gttaaagtcc
     1501 cgatcagaaa gttgaagatc aatttcaggg ccgaggcctg accgacggcg tcgaacacga
     1561 tcctcctgtt cgaatagaga tcgatgaaag cgctgacctc atcgtcgata ttcgcggccg
     1621 gtcccgccat ggcgatcagg gtgccgctgc gagcctcggg acggctgcca agaaccggat
     1681 tttccacata tctgcccccg cggcccgtca cctccctctc gacagccaag ctttgcgacg
     1741 gcgccagcgt cgagatttgt atgaaggtct tgccgtcgag gcgatcgata tcctgaagaa
     1801 tggcgacgat ggatgcgaaa tccgaagtta ccgccagaac gaatcgggcg ccattgatcg
     1861 cctgcgttac gtcgttgaaa aaatgaacct tattcgtgcc ctccggcggg gagcgtctgg
     1921 catagacgct gacgcggctg cacacttggc tgagccggtc ggcgagagct tcagccaggc
     1981 gtccatagcc caaaatgcaa atctgaggcg caatggtgtt gtgactgtcg ctcatttcag
     2041 ctccgaccaa gccaacattc gggatcgcag cgcccccggg ttgcgacagc gcttcgggtg
     2101 ccggaaaaaa tggccaaccg gcatgcgatc ctgttgtaac acagcaagaa gctgccgcat
     2161 ccttggacct gactgcccgt tgcttccgtt ggcgaagacc ttgcacatgc gcatcgtacg
     2221 ataggcggca tctcgctcga acccttgatc ggctttgcgt tttcctccgc atcgctcggg
     2281 cgggatctta tgagaatgcc ctttgccaag aggttaattg cgataagcta ttgatttaaa
     2341 atataaaaat accggaaagc acttacgccg cagcgtggac gaaaggcgtg acatggttaa
     2401 caagcgaagc ctcaacatat ttaacggaca tttgctcgtc ccagtaaagg gaaaaattcc
     2461 ggcattaaat tctatttaat gggcgatttg ttaagtataa aatgacgatt gatttcagta
     2521 agttaggatt agattgatgg cgtccaaact taaggtgcgg tggtccaagt ttcgcgcctg
     2581 ataaaagaac ttttaaccaa atacatgacc ggaaataatt ccggcgcgtg gaaattaacc
     2641 accaagggtt aagtgcgcca cgagggcctt gacgtctact ggaggaaagc tccatccgga
     2701 gacatatttt tgggctccgg tagctgacaa acggcttggc cgggcaactg gctgcgacga
     2761 agacgcgtga caaaggccgc ccttgggcgg tcgaccgagc ttgtcctttg gacgactgcg
     2821 gcgatctccc gttgtgggcc ggtcatgata tctgccatca ttccggctgt tcagtaccat
     2881 tctgccgatg acgacacgct gacagctcct tgagagacgc catgaccgct gacacttttg
     2941 acctgatcac ccgctattat gccgccttca atgccggaga catggagacg ttcctatcga
     3001 tgctgagcga cgatgtcgtg cacgacatca accaggggga gcgagaagcc ggcaaagtcg
     3061 cgtttgccgc gttcatggag cgcatgaacc tccattaccg ggaaaagctg gtcgacatca
     3121 ccgtgatggt cgcggccgac ggcacccggg ctgctgctga attcacggtc ttgggcgaat
     3181 acctctcgac agacgagggg ctgccccctg ccgaaggaca aacctaccgc ctgaacgcgg
     3241 gcgcgttttt tgttgtccgc gacggcaagg tggcccgcgt caccaactat tacaacctcc
     3301 aggattggct gagacaggtg ggcggctgaa cgctgcgcgc cgacggcgct cattcttagt
     3361 cgttcacaat cgggaaaaag gcctggtccc ggtaggcgcg gaggatgagg gggaaaaaac
     3421 gcttgtggcc gcgccgccaa tcctccacct cgttgacgtc gctgatccag cggtaagccg
     3481 aatgctcgtg gctgacggtc acttcggcat catttggcaa tgactgtgcg ccgacgcaaa
     3541 tgttgatctg gctgatcgcg ccgggatggt tcagggggct gcgatgccca accgtgatga
     3601 tgcccagagg ggttatttga agccccgtct cctcacgggt ttcgcgctga gcggtgtcga
     3661 aaggagattc tccgacgtcc atccagccgc cggggaggca ccatttgcca ttcggcagtt
     3721 gcaggaccag gatttcccca ttgtgattgg gaacggcgat atccacgccg accttgggtg
     3781 tgattgaccc gtggtctctg aggaacattt cgcgtattgc ttcagggtac aggcctgtaa
     3841 ttgaggcgta tttctcgctg gccaggttga gcagcttctg ataacgggcg cggtcgtagt
     3901 cattctcggc gtaggaaaga ccttcctggg ctgtagcacg cagaatatcg agaagcattt
     3961 ccaagtttaa cgtcggagtt ccaacagggg ttctactttc catttagccc aacttccttg
     4021 atttcatata catccaatat ttctagcgca gagttgccca atggagcgcc aacttggacg
     4081 gggataccca cctctggtcc cgataatgat atgcggtgca tgcggcacga tcatcagcat
     4141 aatctgtgat tgctgtcagg tgcattggcg tgttcttata tgaagcgcca taagtcacga
     4201 cgcttttgcc catggccgcc cttggtggca acaatttact tatatattta cttatggcat
     4261 gtttacaaaa cgaaaccaat tttagccgat cgttaaccct cacgggctgg cgcccatgga
     4321 gaagctccag ctgataatat gagtcgaacg ttctgacggt tgctattact gcgtggtccc
     4381 ctgattgctc attctctcgt catgcctctc accacccgct tcgctccgag cccaaccggt
     4441 tatctgcatc tcggccacgc ttattccgcg cactttgctt atgaggcggc gcggactgaa
     4501 gcggggacgt tcctgctcag ggtcgaggac atcgatccca tacgatgcag gcccgaattt
     4561 gcgcaggcgc ttttcgagga tttgcgctgg ctggggctga catgggaaga gccggtacag
     4621 cggcaatcgg agcatatgga ggattatgcg gcagcgctcg cgaagctacg gaccatgggt
     4681 ttgctgtacc cctgcttttg cacccgccgc gagatcgagc atgaagttat ggccgctgcc
     4741 gccgcgccgc atttgacccc tcaaggcccg gacgggtggg tgtatccggg gacgtgccgc
     4801 aatcttgatc ccggcgaacg cgcgcggcgg ctgctccaga acgaaggggc gaactggcgg
     4861 ctggatgtcg caaaggcgtt tgcgataacg gggccgctat attggagcga ccgcgccgca
     4921 ggacgcgtcg aggcggcccc tttagatttc ggcgatgtgg tgctggcccg caaggatgtg
     4981 ccggcgagct atcatctcaa cgtcaccgtg gacgatcacc tccagcacgt cacgcttgtc
     5041 acgcgcggca aggatctctt ccgcgccacg gacattcacg tgctgctgca ggcgatcctt
     5101 ggctacgcaa cgcctgacta tcaccat
//
LOCUS       DATMGX010000794         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_47710, whole genome shotgun sequence.
ACCESSION   DATMGX010000794 DATMGX010000000
VERSION     DATMGX010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47710"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VNR65_13025"
     CDS             complement(<1..277)
                     /locus_tag="VNR65_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19640.1"
                     /translation="MSKGWSVQYRLPSVDEIEEDTSLIGDVLAYMNSPQGELFLEVGE
                     ATSGVLESADVDAKERQIIWDDGEPLSISASAQRIGVEYPEYPLDMIE"
     gene            complement(301..615)
                     /locus_tag="VNR65_13030"
     CDS             complement(301..615)
                     /locus_tag="VNR65_13030"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HWJ19641.1"
                     /translation="MATTRIEQIHKRIERIKAELVAIDEMRPGSVSKQFKEPDLERGA
                     YYQLNYFSGGRSRTDYVAPAVLRDVRRQVSNYKRFKALTAEWVELSIEQSRLKIKAAS
                     KL"
     gene            683..2083
                     /locus_tag="VNR65_13035"
     CDS             683..2083
                     /locus_tag="VNR65_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ19642.1"
                     /translation="MPLWVLWWNALHLLQPAFSRLRAFFWFATTVAGFTVRTEHLGVS
                     SIVRALGLNAGCYDNLNKNMHSSAVRIDDLNALWTRVVLKLFPSPVRVNGRRVLVGDG
                     IKAPKRGKKMPGVKLLHQQSESNNKSEYIMGHSIQAVCVLVHVLNSVVAVPLGMRIHE
                     GLVWSNRDRRTLLDKMLSLINLLGVNEPFYFVGDAYYAAGKMAQGLLEQGNHLITRVK
                     SNAVAHEPYICERQKRRGRPRLYGPKIALKTLINATTGIQQTPSLVYGEQDVIISYVV
                     RDLLWRPTGQLARFVAVMHPTRGACLLMSTDTTLGAADIIRLYGLRFKIEHTFKQAVR
                     SIGTFAYHFWMKEMKPLRRRSGDQYLHRETQKYRDDIKRKTHAYHVFMQAGVVAHGLL
                     QYLAAVAPTQVWNSFGSWLRTIRPGVAPSEFVVAGALRRTLPEFLLNSRQEAAIAKFI
                     GDHQDRDIMAMFGIAA"
BASE COUNT          455 a          610 c          564 g          480 t
ORIGIN      
        1 tctcgatcat gtcgagagga tattcgggat actcaactcc aatgcgctgc gctgacgcgg
       61 atatggagag cggttcgccg tcgtcccaaa tgatctggcg ctcttttgca tcgacatcgg
      121 cgctttcgag cacgcctgat gtggcttcac cgacttcaag gaagagttcg ccttgcggag
      181 agttcatata cgccagaacg tcgccgatga gcgacgtgtc ttcctcgatc tcatcgactg
      241 atggcagtcg gtattggacg ctccagccct tgctcacggg ctaaatatac cataaggatc
      301 tcaaagtttg ctagcggctt tgatcttgag gcggctctgc tcgatgctga gctcgaccca
      361 ttctgccgtg agtgctttga accgcttgta gttgctgact tggcggcgga catcgcgtaa
      421 cactgcgggc gcgacatagt cggttcggct tctgcctccg gaaaagtagt tgagctggta
      481 gtaggcaccg cgctcgaggt cgggttcctt aaattgcttg ctcactgacc ccggtcgcat
      541 ctcatcgatc gcgacgagtt ccgccttgat ccgctcgatg cgtttgtgga tctgttcgat
      601 tcgcgttgtc gccatgttta cagtatagct tatatctgct acactgtaaa caattacgcg
      661 gcagctaaag atcggggttc cgatgccgtt gtgggtcttg tggtggaacg cgctgcacct
      721 cttgcagccg gcgttctctc gattacgcgc gttcttttgg ttcgcgacga ccgtggccgg
      781 ctttaccgtg cggaccgaac acctgggcgt ttccagcatc gttcgggcgc tcggacttaa
      841 cgccggatgc tatgataatc tgaacaagaa catgcacagt tcagccgtgc gaatcgatga
      901 cctcaatgca ttgtggacac gtgtggtctt gaaactcttc ccaagtcccg tacgcgtcaa
      961 cggccgccgc gttctcgtcg gagatggcat caaagcgccc aagcgcggca agaaaatgcc
     1021 gggcgtcaag ctgctgcatc agcagtccga atccaataac aaatcggaat acatcatggg
     1081 ccactcgatc caggcagtat gcgtgctcgt acacgtgctc aacagcgtcg tggctgtgcc
     1141 cttgggtatg cgcattcacg aagggctcgt ctggagcaat cgcgaccgcc gcacgctgct
     1201 ggacaaaatg ctttcgctca ttaatcttct gggcgtcaac gaaccattct actttgtcgg
     1261 tgacgcctat tacgctgccg ggaaaatggc tcaagggcta cttgagcaag gtaatcacct
     1321 catcacgcga gtcaaatcca atgccgttgc tcacgaacct tacatctgcg agagacaaaa
     1381 acgacgaggc cggccgcggc tctatggacc caagatcgca ctcaaaacct tgatcaacgc
     1441 gaccaccgga atacaacaga cgccaagcct cgtttacggc gagcaagacg tcatcatcag
     1501 ctacgtcgtt cgtgatctct tgtggcggcc aacggggcaa ctcgcacgat tcgtcgcggt
     1561 catgcacccg acacgcggag catgcctgct catgtcgacc gacaccaccc ttggcgcagc
     1621 agacattatt cggctctacg gcctgcgttt caaaatcgag cacaccttca aacaagcggt
     1681 ccgttcgatc ggcacgttcg cgtatcactt ttggatgaaa gagatgaagc cgctacgccg
     1741 ccgcagtggc gatcagtatc tccatcggga gacccagaaa tatagggacg atatcaagcg
     1801 caagacccac gcctatcatg tattcatgca ggccggtgtc gtcgcgcacg ggctccttca
     1861 atacttagca gccgtcgcgc cgacccaagt gtggaattca ttcggatcgt ggctgcgaac
     1921 gattcgacca ggcgttgcgc cttctgaatt tgtcgttgcc ggcgccctac gacgcacgct
     1981 accggaattt ctcctgaata gccgccaaga ggctgccatc gcgaaattca tcggcgacca
     2041 tcaagaccgc gacatcatgg cgatgtttgg aatcgcggca tgataaaact cgccactcac
     2101 gagtatatc
//
LOCUS       DATMGX010000795         5327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_456292, whole genome shotgun sequence.
ACCESSION   DATMGX010000795 DATMGX010000000
VERSION     DATMGX010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456292"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /gene="bamA"
                     /locus_tag="VNR65_13040"
     CDS             <1..2085
                     /gene="bamA"
                     /locus_tag="VNR65_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HWJ19643.1"
                     /translation="EGGAVIVEVAEAQLIRNVAFEGAKDVTSDTLAKEAQLKASGLYT
                     SARAQADVQRVLEVYRRQGYYAAQVEAKLIELDHNRVDLVYEIREGAVTKVAGINFIG
                     NKSFSDAELRGAITTTESGLLDFLKPTSIYDPDRLNLDRELLRRFYLRNGFADMRIVS
                     AGADADQEKKGFFLTFVVEEGARYTFGTIDVELALKSLNADALRKRVLGNSGDIYNAD
                     LVEKSVEALTAAAAEEGEPFGQIRPRIDRDTKARTISVVYAVEQGPREYIERIEITGN
                     HITKDEVIRREFRIAEGDAYNKVMVEQAKQRVMRTGFFKTVKIEKENGSTADRTVLTV
                     NVEEQQTGELSFAAGYSTANGVIGDVTYTERNLMGTGQFLQVQLNGSLSGSEGVTVSW
                     TEPRFLDRNLSFGVDAFIKNSDYTQASGYTVAGYEDFRVGGALRFGVPVTDEFSVGFN
                     YTLMLDDVYNIDPNASLAVKQIAGTAIISSVGYNLIYDTRNNRKKPTRGFFFKGTQDF
                     AGAGGDVNYIRSTAEMRAYYPISDEITLAGRTTGGTIMGWGGQGVRVVDAFYKGNDTI
                     PGFQPAGLGPRDAATGDALGATTFYSATTELRFPLPFVPQDLGLSGAVYTAAGSAFGT
                     DAQKFAKAYAAQHGTANTLVIQDSSALRSSVGASLIWDSPVGPLRADFSNVLTKAPQD
                     KTQVFGFGYNPW"
     regulatory      2229..2315
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2457..3602
                     /gene="gcvT"
                     /locus_tag="VNR65_13045"
     CDS             2457..3602
                     /gene="gcvT"
                     /locus_tag="VNR65_13045"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF010093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWJ19644.1"
                     /translation="MSEAEALINPVSSGANLCVTPLNALHRELGAKLVPFAGYEMPLQ
                     YASGIVKEHIHVRTAAGLFDVSHMGQAKLAGGDFETTAAALEKLVPGDVLGLRPGQMR
                     YTQLLNESGGIIDDLIVARDADGVGVSLVVNASRKEIDFAHIAERLPLTTRLRPEPDR
                     ALLALQGPQAAAVLSRLIPGVEALGFMTCGVFGPVSVSRSGYTGEDGFEISVEADGAE
                     ALARRILSAPEVLPVGLGARDTLRLEAGLPLYGQDMDETVSPVEAGLAFSIGKRRRSQ
                     GGFLGSERVLRELAEGPKRLRVGLKLEGRAAARHGMRILEPAGKPSGEITSGAFTPTA
                     AASIATGYVHTEVSKPGTRLAIEIRGAAIPAQVNNLPFVPHRYVRKA"
     gene            3619..3996
                     /gene="gcvH"
                     /locus_tag="VNR65_13050"
     CDS             3619..3996
                     /gene="gcvH"
                     /locus_tag="VNR65_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWJ19645.1"
                     /translation="MALIKYTEDHEYISVDGDIGTVGVTNYAQEKLGDVTFVELPASG
                     EKVKQGGPIAVVESVKAASDIYAPASGVIIEVNATLNDKPELINQDAEGAAWILKLQL
                     TDTSELDKLMDLDAYLAYAKSEG"
     gene            3996..>5327
                     /gene="gcvPA"
                     /locus_tag="VNR65_13055"
     CDS             3996..>5327
                     /gene="gcvPA"
                     /locus_tag="VNR65_13055"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWJ19646.1"
                     /translation="MRYLPHTEADRRSMLDAIGVTGIEALFADVPASKLRSELPDLPR
                     RQSEMAVERALSAMAARNMSAGSVPFFAGCGAYKHHVPATVDYVIQRSEFMTAYTPYQ
                     PEIAQGTLQYLFEFQTQVAALTGMEIANASMYDGSTAAAEAVLMAHRLTKRKKAVLAG
                     GLHPHYRAVIETVSHHSGFQVDAAAADPATTFDVTCDADTSCIVVQMPDVFGRVNDLA
                     AIAAKAHECGALLIVAVTEAVSFGALKSPGAMGADIVVAEGQSIGNGLNFGGPYLGLM
                     ASRRKFVRQMPGRLCGETVDEDGRRGFVLTMSTREQHIRREKATSNICTNSGLCALAF
                     SAHLTLLGEAGLRKLAALNHANAVALAKALGGVPGVEVITPAFFNEFTIETPKASGAV
                     IDALADRGIIGGVPASRLWPSVTALENLIVVAATEVTTPNDIAAYKTKLIEV"
BASE COUNT         1090 a         1570 c         1647 g         1020 t
ORIGIN      
        1 gaaggcggcg ccgtcatcgt cgaggttgcg gaagcgcagc tcatccggaa cgtggcgttc
       61 gagggggcca aggacgttac ctccgatacg ctggcaaaag aagctcagct caaagcttcc
      121 gggctataca cgtcggctcg cgcccaggcc gatgttcagc gggttctcga agtctatcgc
      181 cggcagggtt actacgccgc acaggttgaa gcaaagctca tcgaactcga ccacaaccgc
      241 gtcgatctcg tctacgaaat tcgcgaaggc gcggtgacaa aggtcgccgg cataaacttc
      301 attgggaaca agtccttcag cgatgccgaa ctgcggggcg cgataacgac gacggaatcg
      361 gggcttctcg atttcctgaa gccgacttcg atctacgacc ccgaccgcct taatctcgac
      421 cgcgagcttc ttcgacgctt ctatctgagg aacggtttcg ccgacatgcg catcgtctcc
      481 gcgggcgcgg acgccgatca ggaaaagaag ggtttcttcc ttaccttcgt tgtcgaagag
      541 ggcgcgcgat acactttcgg gacgatcgac gttgagttgg cgctcaagtc tttgaatgca
      601 gatgcgttgc gaaagcgcgt gctcggtaat tccggggaca tctataacgc ggacctcgtt
      661 gaaaagagcg tcgaagcgtt aacggcggca gctgcggagg agggagagcc cttcggacag
      721 atccgcccga ggattgaccg cgatacgaaa gcccggacga tttcggtcgt gtatgcggtc
      781 gagcaaggtc cacgcgaata tatcgagcgg atcgagataa ccggcaatca catcacgaag
      841 gacgaggtca tccgccgcga attccgcatc gccgagggcg acgcctacaa caaggtgatg
      901 gtggagcagg cgaagcagcg cgtgatgcga acgggcttct tcaagacggt gaagattgaa
      961 aaggagaatg ggtctacggc ggatcgcacg gtcctgacgg tcaatgtgga agaacagcag
     1021 actggcgagc tttccttcgc ggcgggctac tcgaccgcca acggcgttat cggcgatgtg
     1081 acctatacgg aacgcaatct catggggacg gggcagtttc tccaggtgca gctcaatggg
     1141 agcctttccg gcagcgaggg ggtgacggtg agctggaccg agccgcgttt tctcgaccgc
     1201 aacctctctt tcggcgtgga tgccttcatc aagaactccg actacacgca agcctccggc
     1261 tataccgttg cgggctacga ggatttcagg gtggggggcg ctctccgctt cggcgtgccc
     1321 gtaacggacg agttttccgt cggcttcaat tacactctga tgcttgacga cgtgtataat
     1381 attgatccga acgcctctct tgccgtgaag cagatcgcag gcacggcgat tatttcctca
     1441 gtcggctaca acctgatcta cgacacgcga aacaacagga aaaagcccac gcgcggtttc
     1501 ttcttcaagg gaacacagga tttcgcaggc gcgggcgggg acgttaatta catccgttcc
     1561 accgccgaaa tgagagccta ttaccccata tccgatgaga tcacgcttgc cgggcgcacc
     1621 accgggggta cgatcatggg ctggggtggc cagggcgttc gcgtcgtgga tgccttctat
     1681 aagggaaacg atacaatccc cggctttcag cccgcgggcc tcggtccccg cgatgcggcg
     1741 acgggcgatg cgctcggagc caccacgttc tattcggcaa caacggagtt gcgatttcca
     1801 cttccctttg tgccgcagga cctcggtctc agtggcgcgg tctacacggc tgcaggtagc
     1861 gctttcggca cggatgcgca gaaattcgcg aaagcctacg cagcgcaaca cggcacggcg
     1921 aacactcttg tcatccagga ttcttccgct ctgagatcat cggttggcgc aagcctgatt
     1981 tgggattcgc ccgtgggtcc gctccgcgcg gacttttcta acgtcctgac gaaagcacct
     2041 caggataaga cgcaggtttt cggtttcggc tacaacccct ggtgatgctg ggcaggtcga
     2101 gcggcccatg gaacaggcaa agcgtggcgc ggttgtcgca tcctcgccgg ccaagtgtgt
     2161 attcaagaat ttattacccc gccagaggtt gacaggacgg gccgagcagg cgacttaaaa
     2221 ggggctgaac gatgcaagcg ggagagacca gcagtgctgg cgccgaagga gcaaccgccc
     2281 cggaaactct caggcaaaag gaccgcgcgc agacagcact ctggaaagca gccgccgcaa
     2341 cccggcgtct caccgaaggg gtagacccgg aaatcccggg tgaaatctct caggtcccgt
     2401 gacagagggg gcgcagtgcc gcgaaagcgg agcgccttaa cgttacggaa ccgatcatga
     2461 gtgaagccga agccctcatt aaccctgtat cgtccggcgc gaacctttgc gtgacgccgc
     2521 ttaacgcgct ccatcgcgag cttggggcca agctggtgcc ttttgctggc tatgagatgc
     2581 ccctccaata tgcaagcggc atcgtcaagg agcatatcca cgttcgcacc gcagccggac
     2641 tcttcgacgt ctcgcatatg gggcaggcga agctcgctgg aggagatttc gaaaccacgg
     2701 ccgccgcgct cgaaaagctc gtccccggcg atgtgctcgg gcttaggccc ggccagatgc
     2761 gctacacaca acttctcaac gaaagcggcg gcataatcga cgatctcatt gtggcgcgcg
     2821 atgcagacgg cgttggcgtc tcgcttgtgg tcaacgcctc gcggaaggag atcgactttg
     2881 cgcatatcgc tgagcgactg cctttgacta caaggctccg gcccgaacct gaccgtgcgc
     2941 tcctcgccct gcaggggccg caagccgccg ccgtgctttc gcgattgatt cccggcgtcg
     3001 aggcgcttgg cttcatgaca tgcggcgtgt tcggccccgt ttcggtttcg cgctccggct
     3061 atacgggcga agacggtttc gaaatctcgg tcgaggctga tggcgcggaa gcactggcta
     3121 ggcgaatcct ttcagcgccc gaggtcttgc cggttgggct gggcgcgcgc gatacattgc
     3181 gtctcgaagc cggccttccc ctttacggtc aggacatgga tgaaacggtg tcgccggtcg
     3241 aggccgggct tgccttctcg attggtaagc gccgccgctc acaaggcggc ttcctcggct
     3301 ccgaacgtgt gttacgggag cttgcggagg ggccgaagcg ccttcgggtt gggctgaagc
     3361 tggaaggccg cgcggccgcc cggcatggca tgagaattct ggaacctgcc ggaaagccca
     3421 gcggcgagat aacaagcggt gcttttacac cgacagcggc tgcctcgatt gcgacgggtt
     3481 atgttcacac agaggtaagc aaacctggca cgagacttgc gatcgaaatt cgtggcgcag
     3541 ccattccggc gcaagtcaac aacctacctt tcgtccctca tcgttacgtt cgcaaagcat
     3601 agaacaaggt cagacactat ggcgctcatt aaatacaccg aagatcacga atatatcagc
     3661 gttgacggcg acatcgggac tgtcggcgtg acgaattacg cccaggaaaa gctcggcgac
     3721 gtgaccttcg ttgaacttcc ggcgtcggga gaaaaggtta agcagggggg cccaattgct
     3781 gttgtggagt cggtcaaggc ggcaagcgat atctatgccc cggcgagcgg cgtcatcatc
     3841 gaagtcaacg ccacgctcaa cgacaagccg gaactcatca atcaggacgc cgagggcgca
     3901 gcctggattt taaagctcca gctcacggac acgagcgagc tggacaagct gatggacctt
     3961 gacgcctatc tcgcctacgc gaagtcggag ggctgatgcg ctatcttccc cacactgagg
     4021 ctgatcgccg ctccatgctc gacgcgattg gtgttaccgg catcgaggcc ctttttgcgg
     4081 acgtacccgc gtccaaattg cggagcgaat tgcccgatct gccgcgcagg cagagcgaaa
     4141 tggctgtgga gcgcgcgctt tctgcgatgg cggcgcgcaa catgtcggcc ggttccgtgc
     4201 cgttcttcgc gggctgcggt gcttacaagc accacgtccc agcaacagtc gactatgtga
     4261 tccaacgctc cgagttcatg acggcgtaca cgccctatca gccggaaatc gcccaaggca
     4321 cgctgcaata tctgttcgag ttccagacgc aggtcgcggc cctgaccggc atggaaatcg
     4381 cgaacgcctc gatgtatgac ggatcgacgg ccgcagccga ggcggtgctg atggcgcatc
     4441 ggttgacgaa gcgcaagaag gccgtgcttg cgggcggctt gcatccgcat taccgcgctg
     4501 tgatcgagac ggtttctcat cattccggtt tccaggtgga tgccgcggcg gccgatccgg
     4561 cgaccacgtt cgatgtgacc tgcgatgccg atacgtcctg catcgtcgtg caaatgcccg
     4621 atgtgtttgg acgcgtgaac gacctcgccg cgattgcggc caaagcgcac gaatgcgggg
     4681 ccttgctgat cgtcgccgtc accgaggcag tctcgttcgg agccctcaaa tcgccgggcg
     4741 cgatgggggc agatatcgtc gtggcggaag gacaatccat cgggaacggc ctcaacttcg
     4801 gcggccctta tctaggtctt atggcctccc gccggaagtt tgtccggcag atgcccggcc
     4861 ggctctgcgg cgagaccgtc gacgaggatg gacgccgagg ctttgtactg accatgtcga
     4921 cgcgcgagca acatatccgc cgcgaaaaag cgaccagcaa catttgcacg aattccggcc
     4981 tttgtgcgct cgcgttctca gcgcatctga cgcttctcgg cgaggccggg ctgagaaaac
     5041 ttgccgcgct caatcatgcg aacgccgtcg cactggccaa ggccctgggc ggggttccag
     5101 gcgtcgaggt gatcacgccc gctttcttca acgagttcac gatcgagacg ccgaaggcga
     5161 gcggcgctgt catcgatgcg ctcgcggatc gaggcattat cggcggcgtt cctgcgtccc
     5221 gcttgtggcc cagcgtgacc gcgctcgaga atttgatcgt cgtcgcggcg acggaagtaa
     5281 caaccccaaa tgatattgct gcctacaaga ccaaacttat tgaggtc
//
LOCUS       DATMGX010000796         3899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_79990, whole genome shotgun sequence.
ACCESSION   DATMGX010000796 DATMGX010000000
VERSION     DATMGX010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79990"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VNR65_13060"
     CDS             complement(<1..999)
                     /locus_tag="VNR65_13060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HWJ19647.1"
                     /translation="MTSLRVLAVASEIYPIIKTGGLADVVGALPIALRAYGIETRTLV
                     PGYPDVIKALPAAEVLLHWPHFYGGPIRLLGGSHEDLDLLVLDAPHLFARPGNPYVTP
                     DGKDWPDNGVRFAALSRMAAKIGQGAIPSFVPDIVHAHDWQAGLAPAYLHYANGPRPG
                     TVMTVHNLAYQGKFPHEILDTFGLPPESFTIHGVEYYGMVSFLKAGLQFADRITTVSP
                     TYAIEIQGDEGGMGLGGLLRERSQVLSGILNGIDTSVWNPETDPHIAARFSAKELESR
                     AANRAALQRRLGLDPSPDALLLGVISRLSWQKGLDLLLDNVATITGEGMQLALLGSG"
     gene            complement(996..2258)
                     /gene="glgC"
                     /locus_tag="VNR65_13065"
     CDS             complement(996..2258)
                     /gene="glgC"
                     /locus_tag="VNR65_13065"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWJ19648.1"
                     /translation="MATVINEPLSRHALAFVLAGGRGSRLLELTDRRAKPAVYFGGKS
                     RIIDFALSNAVNSGIRRIAVATQYKAHSLIRHLQMGWNFFRPERNESFDILPASQRIS
                     ETNWYLGTADAVYQNIDIIEPHRARFVVLLAGDHIYKMDYELMLRQHVEQRADVTIGC
                     LEMPRSESSGFGIMHIDDHGVIQSFLEKPADPPPMPGKPDKSLASMGIYIFDSKFLFD
                     ELRRDANDPNSSHDFGKDIIPYIVKNGRAVAHQFSSSCVRSGDDPRTYWRDVGTVDAY
                     WAANIDLTDVLPELDLYDRAWPIWTYAEITPPAKFVHDEEGRRGQAVTSLVSGGCIIS
                     GAALSRSLLFTGVHINSFANVENAVVLPYVNVGRHARLKNVVIDRGVRIPDGLVVGED
                     PELDAKRFRTTPQGISLITQPMIDGLNA"
     gene            2816..3454
                     /locus_tag="VNR65_13070"
     CDS             2816..3454
                     /locus_tag="VNR65_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19649.1"
                     /translation="MSVIGGRAENICSFRVFLSLTRSGSSKEGRYFILIAQDQCWQSA
                     VLLILFLRIVYFAPETSMTEPTKETVIIVHGTWAAPKLEEPAKRRWYEPVDDRPGDEP
                     FPTKLDTALQERGSPARCWKHCTQDDQIFHWSGDNSWIARTRAASELADYVAKLRNEG
                     WCCHIVAHSHGGNVLVDALSQLMAPSNFVEPRGNLATLGTPFMDAMSPVLDR"
     gene            3610..>3899
                     /locus_tag="VNR65_13075"
     CDS             3610..>3899
                     /locus_tag="VNR65_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19650.1"
                     /translation="MDWLSRSGTEIYMAIVFESSPTSVSRLIFWDLIEIPELVAVLYI
                     ANSVLFRFAQAGRSAFWLHLAKPGQAKPQPILAIGSPMDEAWQVLHYVRQCD"
BASE COUNT          846 a         1074 c         1173 g          806 t
ORIGIN      
        1 gccactgcca agcaaggcaa gctgcattcc ctcgcctgtg atggtggcga cgttgtcgag
       61 aagcaggtcc aatcccttct gccaggacag ccggctgatg acgccgagca gaagcgcatc
      121 aggagatgga tcaaggccga gccgccgctg cagggcagct ctgttcgccg cacgggattc
      181 cagctccttt gcgctgaagc gagcggcgat gtgcggatcc gtttcggggt tccagacgga
      241 ggtatcgata ccgttgagga tcccgctcag gacctgcgat cgctcacgca acaatccgcc
      301 cagtcccatg cccccctcat ctccctgaat ctcgatcgca taggtaggag agaccgtcgt
      361 gatgcgatcc gcaaattgca ggcccgcctt caggaagctg accattccgt aatattcgac
      421 gccgtgaatc gtgaaggact cgggaggcaa gccgaacgta tcgagtattt catgcggaaa
      481 cttcccctgg taggccagat tgtgaaccgt catcacggtg ccgggccggg gaccattggc
      541 atagtgcaaa taggcaggcg cgagtcccgc ctgccaatcg tgggcgtgca cgatatcagg
      601 cacgaatgac ggaatggcgc cctggccgat tttggccgcc atccgggaaa gcgccgcaaa
      661 gcgcacgccg ttgtcgggcc aatccttgcc gtccggcgtg acataggggt tgccgggccg
      721 cgcaaaaaga tgtggcgcat cgagcacgag cagatcgagg tcctcatgtg agccgccgag
      781 caggcggatc ggcccgccgt agaaatgcgg ccaatggagg agcacttccg ccgccggaag
      841 cgccttgatg acatcgggat atcccggcac cagggtccgc gtttcgatgc cgtacgctcg
      901 caacgcgatc gggagcgcgc cgacgacgtc ggcaaggccg ccggtcttga tgatcggata
      961 gatttccgag gcgaccgcaa gaacgcgaag cgacgtcatg cgttcagccc gtcgatcatc
     1021 ggctgcgtaa tgagagagat gccctgcggg gtggttcgaa accgtttcgc atcgagttcg
     1081 ggatcttctc cgaccaccag accgtccgga attcgaacac ctcgatcgat gacgacattc
     1141 ttcagccgcg catgccggcc cacattcacg taaggcagga caaccgcatt ttccacgttg
     1201 gcgaacgaat tgatgtgcac gccggtaaag aggagggagc ggctcagagc tgcgccggat
     1261 atgatgcatc ctccggaaac aagtgaggtc accgcctgtc cgcggcggcc ctcctcatcg
     1321 tgaacaaatt tggcgggagg cgtgatctcc gcataagtcc agatcggcca tgcgcggtcg
     1381 taaagatcaa gctccggcag gacatcggtg agatcgatgt tggcggccca gtaggcatcg
     1441 acggttccca cgtctcgcca gtaggtgcgc ggatcatcgc cggacctgac gcaggagcta
     1501 gagaattgat gggccaccgc ccggccgttt ttcacgatat agggaatgat gtccttgccg
     1561 aagtcatggc tcgagttcgg gtcattcgcg tcgcgccgca gctcgtcgaa caggaacttg
     1621 gaatcgaaga tgtagattcc catgctggcc agtgatttgt caggcttgcc gggcattggc
     1681 ggcggatcgg ccggtttctc gaggaaggat tggatcacgc catggtcgtc gatgtgcatg
     1741 atcccgaagc cgctcgattc cgagcgcggc atctccaggc aaccgatcgt gacgtccgcg
     1801 cgttgctcga catgctgcct cagcatgagt tcgtaatcca tcttgtagat atgatctccc
     1861 gcgagcagca cgacgaatct ggctctgtgc ggctcgatga tatcgatgtt ctgatagacg
     1921 gcgtcagccg tgccaagata ccagttcgtc tccgagatgc gctggctcgc cggcaggata
     1981 tcgaagctct cattccgctc ggggcgaaag aagttccagc ccatctgcag atgtcggatc
     2041 aagctgtggg ccttgtattg ggtcgcaacc gcgatacggc ggattcccga gttgaccgca
     2101 ttggagaggg caaaatcgat gatgcgcgat tttccgccga aatagaccgc cggcttggcg
     2161 cgcctgtcgg taagctccaa aagccggctt ccgcgcccgc ccgcaagaac gaaggcgagg
     2221 gcgtggcgtg aaagcggttc gtttatgacg gtggccatct catcctcccg ggagcctcaa
     2281 ggcatggccc cagaaaacct tctcttgtcc ggaaggcgag gttgagggcc actgggccga
     2341 ttgtagcggg acgaatggtc gatcggaaag tggtatgtag tacgtgccgc taccgaggtg
     2401 cggacaccga acattcagga ctgccttgaa atgagccaag ctgaatacgc cgcagtcgat
     2461 tggggcacca gtaggtttcg gctttggctc atcgatcggg ccggcggtgt cctggcggag
     2521 cgacgcagcc aggaaggcac tcaatgtaaa agggccaaat gaccggcctg ggtcaaaggt
     2581 tgacttgtcg ggaatgaccg cgcaaagcgg ccgttcatac gcaaacggga tctcgtggtc
     2641 gataacgaat gagtagaaaa ctatccagtt tccaaccggc aaggagatcg taaaaccttg
     2701 tcagtcggct caggcgcggg cagggcgttc gccaaggggc attcgcggaa tattggatat
     2761 ggaatgaaaa cactcccttc cgcctgaact aagcctagcc aaacggccgc cgaggatgtc
     2821 tgttatcggc ggaagagcgg aaaatatctg ctcgttccga gtatttctca gtttgacccg
     2881 aagcggaagt tccaaagaag ggcgttactt catccttatt gcgcaagatc aatgctggca
     2941 gagtgccgtg cttctaatct tatttctcag gattgtatac ttcgcgccgg aaaccagcat
     3001 gacggagccc acaaaagaga ccgtgattat tgtgcacggt acttgggcag cgcccaagct
     3061 ggaagagccc gcgaaacgca gatggtatga acctgttgac gaccggccag gagacgaacc
     3121 gtttcctacc aagcttgaca ccgcattgca ggagcgcggc tctccggctc ggtgctggaa
     3181 gcactgcacg caagacgatc aaatcttcca ctggtccggt gacaacagct ggatcgcgcg
     3241 cactcgtgcg gcatcggaat tagcggacta tgtcgctaag ctccgtaatg aaggttggtg
     3301 ctgtcacatt gtcgcgcaca gccatggcgg aaacgtcttg gtcgacgcgt tatcacaatt
     3361 aatggcgcca tcgaatttcg tggagccgcg aggcaatttg gcgacattgg gcactccgtt
     3421 catggacgcc atgtcaccag tattagacag atagcgattt ccagcggcat cgcgatgatc
     3481 attatctttg tatatagttt gatttctagg caccgcagca taccggtctc taagccgcaa
     3541 gcgaaatgga agcgttttgg tgcccattac gttttttggt tcctctgcgt attggcgttc
     3601 ggaacaataa tggactggct ttcgcgaagc gggaccgaaa tctacatggc gattgttttt
     3661 gaatcttcgc cgacgtcagt ttcgaggctt atattttggg accttataga gatccctgaa
     3721 ctcgttgcgg tgttatatat cgcaaacagt gttcttttca gatttgcgca ggcaggtcgt
     3781 agtgcattct ggctgcactt ggctaagccc ggccaagcaa aacctcaacc cattctcgcc
     3841 atcggcagcc cgatggatga ggcttggcag gtcctacatt acgtgcgcca gtgtgatga
//
LOCUS       DATMGX010000797         2641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_262421, whole genome shotgun sequence.
ACCESSION   DATMGX010000797 DATMGX010000000
VERSION     DATMGX010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262421"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2253
                     /locus_tag="VNR65_13080"
     CDS             <1..2253
                     /locus_tag="VNR65_13080"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWJ19651.1"
                     /translation="PGCGKPSPARVKSIMATLRQQIATNVLTVTRFRELMKKLRENRP
                     NDDLDLVKRAYEFSQKHHAGQTRASGEPYLVHPLEVGNVLADMKMDSVSIAAGLLHDS
                     VEDTSVTTVEIRQEFGEQVAHIVEGVTKISKIDFSSREEAQAENLRKMMLAMVDDIRV
                     VLIKLADRLHNMRTLEHLEPERQQKIAKETLEIYAPIAHRLGMGKIRGELEDLGFKYL
                     DPIGFEQVRVAVESRRKEGAAFMARVEGVLRDGLKEAGITGRVESRVKRLYSIHRKLI
                     RQRISVDQVYDLYAMRVITNSVQDCYAVLGIIHNLWRPVPGRIKDFIAMPRPNLYQSL
                     HTSVITETGETFEIQIRTEEMHKMCEEGIAAHWKYKDGPVSAEDEQRLAWLRQVVEWQ
                     RDVSDPNEFLSTLKVDLYPEEVYTFTPKGKVLVLPRESTPIDFAYSIHTEVGHSCTGA
                     KVNGRMVPLRHKLHSGDIVEVMTQPGHKPSRDWLAIVKSSRARNKIKHWLNVHQRERA
                     IEIGRKLIEKEARKYRISLKEIKEADLQRAASEYGLGRPDDLMAGIGYGKYSARQVLA
                     KFAPAGAQPMSETEEQAATHGFTSVVRRVFGGDSNAIVVRGSGDLLVYRAKCCNPIRG
                     EAIVGYVTRGKGVAVHSSNCPNVTNLLYEPERRIDVLWAGDKDGQPISYPVKITLLCD
                     DRFGMLKQITAVISDTHTNIRNIEARSQNGQASVDVVLEIADLKHLQKIMDGVRQIPG
                     VHDVQRLQKI"
BASE COUNT          642 a          870 c          672 g          457 t
ORIGIN      
        1 ccagggtgcg gcaagccttc cccggctagg gtaaaatcca tcatggcgac gttgcgtcag
       61 caaatcgcga ccaacgtgct cacggtgacc cgcttccgcg agctgatgaa gaagctgcgc
      121 gagaaccgtc ccaacgacga tctcgacctg gttaaaagag cctacgaatt ctcccaaaag
      181 caccacgccg gacaaacccg cgcttccggc gagccctacc tcgtccatcc gctcgaagtc
      241 ggcaacgttc tcgccgacat gaaaatggat tccgtttcca tcgccgccgg cctcctccac
      301 gactccgtcg aagacacctc ggtcaccacg gttgaaatcc gccaggaatt cggcgagcag
      361 gttgcccaca tcgtcgaagg cgtcaccaag atcagcaaaa ttgatttctc ttcccgagaa
      421 gaagcgcaag ccgagaacct ccgcaaaatg atgctcgcca tggtcgacga catccgcgtc
      481 gtcctcatca agctcgccga ccgcctgcac aacatgcgca ccctcgagca tcttgaaccc
      541 gagcgccagc agaaaatcgc caaggagacg ctcgaaatct acgcccccat cgcgcaccgt
      601 ctcggcatgg gcaagattcg cggcgaactc gaagacctcg gttttaagta tctcgatccc
      661 atcggattcg agcaggtccg cgtggccgtc gagtcgcgtc gcaaagaagg agcagccttt
      721 atggcgcgcg ttgaaggcgt gctgcgcgac ggcctcaagg aagctggcat taccggccgc
      781 gtcgagagcc gcgtcaagcg cctctacagc atccacagga agttaattcg ccaacgcatc
      841 agcgtcgacc aggtttacga cctctacgcc atgcgcgtca tcaccaactc ggtgcaggac
      901 tgctacgccg ttctcggcat catccacaac ctctggcgcc ctgtgcccgg acgtatcaaa
      961 gacttcatcg ccatgccgcg gcccaatctt taccagtcgc tgcacacctc cgtcatcacc
     1021 gagactggcg agactttcga aatccagatc cgcaccgaag aaatgcacaa gatgtgcgag
     1081 gaaggcatcg ccgcccactg gaaatacaaa gacggccccg tctccgccga agacgagcag
     1141 cgactggcat ggctccgtca ggttgtagag tggcagcgcg acgtctccga tcccaacgaa
     1201 ttcctctcca ccctgaaagt agatctctat cccgaagagg tttacacctt cacacccaaa
     1261 ggcaaagtcc tggtcctgcc gcgtgagtcc acgcccatcg acttcgccta ctcgattcac
     1321 accgaggttg gacacagttg caccggcgcc aaggtcaacg gtcgcatggt tcccctccgc
     1381 cacaagcttc attctgggga catcgtcgaa gtcatgaccc agcccggaca caagcccagc
     1441 cgcgactggc tggccatcgt caagtcctcg cgcgcgcgta acaagatcaa gcactggctc
     1501 aacgtgcacc agcgcgagcg cgccattgaa atcggaagaa agctgatcga gaaagaagcg
     1561 cgcaaatacc gcatctcgct gaaggaaatc aaggaggccg acctgcaaag agcggccagc
     1621 gaatacggac tcggccgtcc cgacgacctg atggctggca tcggctacgg gaagtactcg
     1681 gcccgccagg tgctggcgaa attcgccccc gccggcgctc aaccgatgtc cgaaaccgaa
     1741 gagcaagccg ccacccacgg cttcaccagc gtggtgcgcc gcgtcttcgg cggagacagt
     1801 aacgccatcg tagtccgcgg atcgggcgac ctgctcgtct atcgcgccaa gtgttgcaac
     1861 cccattcgcg gcgaggccat tgtcggctac gtcacccgcg gcaaaggcgt tgccgtccac
     1921 tccagcaact gtcccaacgt caccaatctt ctctacgaac ccgagcgccg cattgacgta
     1981 ctctgggccg gagacaaaga cggccagccc atttcctatc ccgtaaaaat cacgctgctc
     2041 tgcgacgacc gcttcggcat gctcaaacaa atcaccgccg taatcagcga cacccacacc
     2101 aacatccgca acatcgaagc ccgcagccag aacggccaag ccagcgtaga cgtagtcctg
     2161 gaaatcgccg acctgaaaca cctccagaaa atcatggacg gagtaagaca gattcctgga
     2221 gtgcacgatg tgcagagatt gcagaagatc tagcgcttcg tatcagggca tcgcttcagc
     2281 gatgccgccc gccttccacc tgtagagacg gggcttgccc cggtttgccg cgttctctac
     2341 gggaaatcag gtccgacgca aacgctataa acagaaccat agcagatccg cagttattat
     2401 aagagcaact atattgttgc atatatagcg tttataattt agcctgaaac taggaagatc
     2461 cgattcggag gcgtcgaaac ccatgccaca agacagcgaa acgtcccccg tggacatttc
     2521 tccaaaagag caacgggaaa tcgtcgacat ctattccaag ctgcgcgaac tggaagcgaa
     2581 gttcatcggc cccgacggca aaaccgagat tctgcccaac aacctttact cgttcttgct
     2641 g
//
LOCUS       DATMGX010000798         2466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_47825, whole genome shotgun sequence.
ACCESSION   DATMGX010000798 DATMGX010000000
VERSION     DATMGX010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47825"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VNR65_13085"
     CDS             complement(<1..288)
                     /locus_tag="VNR65_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19652.1"
                     /translation="MTASPLSPRETRARERMRGRPLWRRGALAIVAILQMLVLFTPPF
                     GARAFAEERIKGEIKVSTEGGYARLAFRFEKEVPANIQITYPIMVVTFKKPV"
     gene            complement(599..>2466)
                     /locus_tag="VNR65_13090"
     CDS             complement(599..>2466)
                     /locus_tag="VNR65_13090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HWJ19653.1"
                     /translation="IGIQRDTASAQYRWTPTDAWDIKADLSHMRRTGTQVDGVVGFFP
                     NTASASSFPYGPTSVPKPVADTTQNYGLNGEYAGTSPWNKKFTFKLAYSGSQYTDDYS
                     SYTVQNPFCTSASTCGTNTATPPGTQSQFARLSTWPSNNANAFSGTLGADLPWQSRYA
                     GTVSYTMMRQNDAFIPMSNNPSITGAANIAATVLPASSLNGAINTLLSNNVVTTKITP
                     ELTNKLSYRYYNFQNDTPQLMFGTPGSTTGWISYDQNVAAERTIQSLSIAYIKQNAGE
                     ELVWRPSREWNLGAAYGYERYDYTQVDATSTDENSGKVFADWKPLRTLTVRASGYYSD
                     RTANNYNYVANVGSIQFPGSTLAQNGYYYNPAYQQLMIDNRQRWKANFAVDVVVARGL
                     TITPTFKYQDDYYGLNPGTQMGLTDSRSWNAGIDATYAINPDMFLMVGYVREYYTQLL
                     LGQSCDLNNVTAFGITNNCVSHSTSTNISVQTNDRTVVDTFTAATRYAAIPDKLDLGL
                     RYTASRATDHQMLNLGSGANPSGGQFPDVTTWFQRLDATAVYTFDKAAVAQAGLTGVV
                     KAKLRYTWERNSVANWQNDPLAPFSPTVSTAGIWLAQDNPNYNVHLLSASLAYTW"
BASE COUNT          494 a          668 c          740 g          564 t
ORIGIN      
        1 gaccggcttc ttgaacgtca ccaccatgat cggataggtg atctggatgt tggccggcac
       61 ttccttctcg aagcggaacg ccaggcgcgc atagccgccc tcggtgctga ccttgatctc
      121 gcccttgatc cgctcttcgg caaacgcgcg ggcgccgaac ggcggcgtga acagcaccaa
      181 catttgcagg atggcaacga tcgccagcgc gccgcggcgc cataacggcc gcccccgcat
      241 gcgctcgcgc gcgcgggtct cccttgggct gaggggcgat gccgtcatgc agccgttcca
      301 accggcgaca atgccggtcg cgacagccta gggtttggcg tttaacgtcg cgttaaccgt
      361 ggggccttcc agccaaacga ttttttcgcg gagagtagat attacaactg gaatcaagca
      421 catccgcgag tattggcgag gccgccacgg gtatctgtaa ggcgcgtaac tttcaattaa
      481 cctgcaatcg gaggggacca tgtgcgtgcg cattttccgc acagtgcagt caccgcaaga
      541 ccgaacttgg tttttagatc atcacggcac gcgtgccacg gcaacccacg gctcgcggct
      601 accaagtgta ggcgagcgac gccgacagca ggtggacatt gtagttcggg ttgtcctgcg
      661 caagccatat ccccgcggtt gatacagtcg ggctaaacgg cgccaatggg tcattctgcc
      721 agttggcgac cgaattgcgc tcccatgtgt agcggagctt ggctttgacc acgcccgtta
      781 agccagcctg cgcgacggca gccttgtcga acgtgtagac cgcggtcgca tccaggcgct
      841 ggaaccaagt cgttacatcg gggaattgac cgccactcgg gttggccccg ctaccgagat
      901 tgagcatctg gtggtccgta gcgcgcgatg cggtatagcg cagacccaag tcgagcttgt
      961 ctggaatagc cgcatatctc gtcgcggcag tgaacgtgtc cacgacggtg cggtcgttcg
     1021 tttgcaccga aatgtttgtg ctggtggaat gggacacaca gttgttggtg atgccgaagg
     1081 ctgtaacatt gtttaagtca cagctttggc ccaacagcaa ctgagtataa tattccctca
     1141 cgtagccaac catgaggaac atatcgggat tgatcgcata ggtcgcgtcg atacccgcgt
     1201 tccaggagcg gctgtcagtc aatcccattt gggtgccggg gtttagcccg taataatcat
     1261 cctgatattt gaacgttggg gtgatggtga ggccacgagc aacaaccacg tcgacggcaa
     1321 aattagcttt ccatcgttgg cgattgtcga tcatcaactg ctgataagcg ggattataat
     1381 aatagccatt ctgcgcgagg gtcgaaccgg ggaattggat gcttcccaca ttcgctacgt
     1441 aattataatt attggcggtt cgatcggaat agtagccgct tgcgcggacg gtgagcgtcc
     1501 tcaatggctt ccagtcggca aagactttgc cggaattctc gtcggtgctg gtggcgtcga
     1561 cttgcgtgta atcatatcgc tcatagccgt aagcagcgcc caaattccat tcgcgcgacg
     1621 gccgccagac caactcttcg ccggcgttct gcttgatata ggctatcgac aagctctgga
     1681 ttgttctttc ggcagcgacg ttttgatcgt aggagatcca acccgtcgtg ctgcccggcg
     1741 tgccgaacat gagctgcggc gtatcgtttt ggaagttgta gtagcggtag ctcagtttat
     1801 tcgttagctc cggcgtgatc ttggtggtca ccacgttgtt gcttaacaac gtattgatgg
     1861 cgccgttcag gctcgatgcc ggaaggacag tggcagcaat gtttgctgcc ccagtgattg
     1921 acgggttatt cgacatcgga atgaacgcgt cgttctgccg catcatcgtg tagctgaccg
     1981 tgccggcata gcggctctgc cacggcagat cggcgcccag cgttccgctg aaggcattgg
     2041 cattgttgct gggccacgtc gatagccgag cgaattgact ttgggtgccc ggtggcgtcg
     2101 cggtattagt gccacaagtg cttgcgcttg tgcagaaagg attttgcacc gtgtaggatg
     2161 aatagtcgtc ggtatattgc gaaccactat aggccagctt gaacgtgaac ttcttgttcc
     2221 acggtgacgt gccggcatat tcgccattca ggccgtaatt ctgggtggta tcggccaccg
     2281 gcttcggtac cgaagtcggg ccatatggaa atgaactggc gctagcggta ttgggaaaaa
     2341 agccaacgac accgtcaacc tgggtgccgg tgcgacgcat atgcgacaag tccgccttga
     2401 tgtcccaggc atcggtcggg gtccagcgat attgcgccga tgcggtgtcg cgttggatgc
     2461 cgatgt
//
LOCUS       DATMGX010000799         4783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_203006, whole genome shotgun sequence.
ACCESSION   DATMGX010000799 DATMGX010000000
VERSION     DATMGX010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203006"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..996
                     /locus_tag="VNR65_13095"
     CDS             214..996
                     /locus_tag="VNR65_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWJ19654.1"
                     /translation="MTDTDSVVCEKRGQAYWITINRPDKRNALNGEVIAGIARGYRTA
                     HDDRDVRVIVLTGAGDKAFCAGADLQNSGAAFAMDFAKPNVDYADLLRLSQNATKPAI
                     ARVGGVCMAGGMGLLCMTDMAVSADHVIFGLPEVKVGVFPMQVMALLQGIAPRRLISE
                     WALTGEPFDARTAQAAGLLNYVVPSAELDAKLDWLISRIVDKSPTAIRRGKYAMRAIA
                     SMSFDESIAYTESQIALLAMTEDAKEGLKAFGEKRRPSWPGK"
     gene            complement(1019..1291)
                     /locus_tag="VNR65_13100"
     CDS             complement(1019..1291)
                     /locus_tag="VNR65_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19655.1"
                     /translation="MIERSMDGLLFFRGGRKLYACVAYNVTQTSAQVHSDELGLLPID
                     FYITFDKFRTIARCRLVWRYQNRIGVAFEKWVIVRGKVAGSDSDPG"
     gene            1421..1810
                     /locus_tag="VNR65_13105"
     CDS             1421..1810
                     /locus_tag="VNR65_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19656.1"
                     /translation="MPALLSNGAVSGRSLCHQRNSFGHFTLAQGKRLGSNSITETVRK
                     ERNMLRKMMLAVLVVTFAGGAALAQEACDSKAVGKDGKPLAGAAKTSFMKKCMADTCA
                     TKAVSQDGKPLSGAAKTSFMKKCEKGA"
     gene            complement(2531..2803)
                     /locus_tag="VNR65_13110"
     CDS             complement(2531..2803)
                     /locus_tag="VNR65_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19657.1"
                     /translation="MRFPSFKLGRADTFLARMQRARSEGYSRGAREAKLFRLSVGKSF
                     DREQVLDWLSTAADEGRAELESQHALEREVEAWDMSFRIAFLVEIA"
     gene            complement(3494..3628)
                     /locus_tag="VNR65_13115"
     CDS             complement(3494..3628)
                     /locus_tag="VNR65_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19658.1"
                     /translation="MGDETASPAVMISDVIPERTYKSDPQEIHPHAGPAERINGDANK
                     "
     gene            complement(3763..4218)
                     /locus_tag="VNR65_13120"
     CDS             complement(3763..4218)
                     /locus_tag="VNR65_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19659.1"
                     /translation="MADDHSDDYKLTLAQQFFGKTVAILRGNPPGSSTDGISEAEATS
                     VVEGHSSFSGSSPPTASHAKAGEGKAQQKQLKTAADLAEMIELDLARHPDCPKVGFRV
                     TVYGGTHWRAMLTITPAAGGIRNPQEWRDLTNDLAERLRQRYNLKVSPP"
     gene            complement(4398..4619)
                     /locus_tag="VNR65_13125"
     CDS             complement(4398..4619)
                     /locus_tag="VNR65_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19660.1"
                     /translation="MTHKVISPSFVIPTALAIVLGIVLAVGGVYLGHYLGPVDQAEAN
                     QSVAGSRGSTELPPDGRLSRIHLLLSNMK"
BASE COUNT          978 a         1433 c         1371 g         1001 t
ORIGIN      
        1 gggtctgggt gaagaaggcc gcgtcgccgg ccgtctagag cctcggttct gattgaatca
       61 gaaccgaagc tctgatcttt tggttgacgc gttttcttca cgcgaaccgg tgcccacatc
      121 gctcgaaaac gccatggaac agctaaatcg cccttgcggt cctgccccgt cctgccgtta
      181 atgtccgccg aaaccggcaa aacgggcagg gcaatgaccg acaccgacag cgtcgtatgc
      241 gagaagcgcg ggcaggcata ctggatcacc atcaaccggc cggacaagcg caacgccctc
      301 aacggcgagg tgatcgccgg cattgccagg ggctatcgca ccgcgcatga cgacagggac
      361 gtccgcgtca tcgtcctgac cggggccggc gacaaggcat tttgcgccgg cgccgatctg
      421 cagaactcgg gtgctgcctt tgcgatggat tttgccaagc ccaacgtcga ctacgccgat
      481 ctgctgcggc tttcgcaaaa tgcgacaaaa cccgcgatcg cgcgggtcgg cggcgtctgc
      541 atggccggcg gcatgggctt gctgtgcatg accgacatgg cggtatcagc cgatcacgtg
      601 atcttcggcc tgcccgaggt gaaggtcggc gtgttcccga tgcaggtaat ggcgctgctg
      661 cagggaatcg cgccgcgccg cctgatcagc gagtgggcgc tgaccggcga gcccttcgac
      721 gccaggaccg cacaggccgc cggccttctc aattatgtgg tgccgtcggc cgagctcgac
      781 gccaagctcg actggctgat cagccgcatc gtcgacaagt cgccgaccgc gatccggcgc
      841 ggcaaatacg ccatgcgcgc gatcgcctcg atgtcgttcg atgaaagcat cgcctacacc
      901 gaaagccaga tcgcgctatt ggcgatgacg gaagacgcca aggagggcct caaggcgttc
      961 ggcgaaaagc gcagaccctc gtggccgggg aagtgagagc gccgtcatcg gacgtggctc
     1021 agcccggatc agaatccgaa ccggcgacct ttcctcgaac aatgacccat ttttcgaaag
     1081 cgacgccgat acggttttga taccgccaaa ccaggcggca tcgggcaatt gttcgaaatt
     1141 tgtcgaacgt gatgtagaaa tctatcggaa gaagtccgag ttcgtctgaa tggacttggg
     1201 cgctggtttg cgtgacgttg taagcgacac atgcgtacag ctttcggccc ccgcggaaga
     1261 acagcaggcc gtccatcgat cgttcgatca tctgcagtcc aatcttcagt acatcgccgc
     1321 gctggctttg gcactttgag gaactacatc atcgacccca gaacggcgaa gatatcgcgc
     1381 cgaacgctgc tgacaatcgc gaacccgaat tacgattaac gtgccggccc tgttgtcgaa
     1441 cggcgcagtt tccggccgtt ctctgtgtca ccagcgcaac agctttgggc atttcaccct
     1501 tgctcaaggc aaacgtctgg gatcaaattc gatcactgaa accgttcgaa aggagagaaa
     1561 catgctcagg aaaatgatgt tggccgtgct ggtcgtgaca tttgccggcg gtgccgccct
     1621 cgcacaggaa gcctgcgaca gcaaagccgt cggcaaggac ggcaagccgc tcgcgggtgc
     1681 ggcaaagacc tcattcatga agaaatgcat ggccgacacc tgcgcgacca aagcggtgag
     1741 tcaggatggc aagccgcttt caggtgccgc gaagaccagt ttcatgaaga agtgcgaaaa
     1801 gggcgcgtag cctggcgccc ttgcgacttg gaaaggccgc cttgggtggc ctttcctttg
     1861 gccattactg tcgccgtttt gtctgcggaa ggctcccgaa aatgacccat accggccgac
     1921 gatctccggc aaatttaact gctcgcgcgg cggtgaagta ggagccgcga ctttctggca
     1981 gtgtttgatc gcattcaaaa aagaccccta gttctcagtc ccgaaccagc aaattcgact
     2041 ggcgttgatc caaatcaagt gggccgatat ctatctttcc tattgttgta gggcggacag
     2101 accaaggctc tgtgcgaatg ttcagctctc acaaaaggga ggccgtcatg gagaaaagcg
     2161 ccgctttccg tgccgaggaa gaaggttaat tgagtgccta cgacgtcggc gttcggtttt
     2221 gagtaagatc agatgaaaca gatcccggtt gacgccatcc tgccgcccgt cgttgcaacg
     2281 ttgttgccaa agaattcaat cgcgcgccgt tgggctgggc acgccgttgg accaactcct
     2341 agaagaaaca ttgcgagggt cccacagcca tggcctcgca gacttgcaag cttcacgctg
     2401 attcctgcga tctgcgcagc aatgttgagt tggcttggtt ttctttcatg gggaatgtat
     2461 tcagcggccc gctggctttg gctatggatt tagcaacgga tcgcttgggc agcttctcga
     2521 agtcgtgtcc tcaggcgatc tcgaccaaaa acgctattcg aaatgacatg tcccacgcct
     2581 cgacttcacg ctccagcgcg tgctgagact ctagttcagc gcggccctcg tcggccgctg
     2641 tacttagcca gtcgagtacc tgctctcggt cgaaggactt gccgacggac agcctaaaca
     2701 gctttgcctc ccgcgctccg cgtgagtagc cctcacttcg cgctcgctgc atccgggcta
     2761 ggaaagtgtc cgcccggcct agtttaaagg aggggaaccg catgcgccat cctgcgggca
     2821 tgctgacggg atgacaagcc ccgtaaaaca cgggttgacc agttgggtgc gaggaaagtc
     2881 aacatccgcg tcaatgacgg gccacagtgg ctgctcggtc gcgtactcct cgatccaggt
     2941 cgacgtcggg agccgaccag acctcggatc tcgggcaaac ccgattgcgg cgaccgatca
     3001 acggcatcgc cccagtggag gggcaatgcc cgcatccctc ctatctcccc tgaacatctt
     3061 ggttagaccc ggcgcgcgtc gtccgtaact cagcgcgcgc cgggccgcac ctagaagttt
     3121 ttatcggact atccataagc ccgcgcccgc ttccggcacc gaccggtcgt gggactttgc
     3181 ttcccgtggc tcgcttcaca ctatgcgggc ggacattgtt ggcctgtcgt gttcagcaac
     3241 cagcatcgct gccttggccc cgctttggaa actcaatcga gtgtcaactt cgcagttggc
     3301 gcagtccccg atgtgatgcg gcagtccctg tcaggagggt cgacgttccg aattagaatc
     3361 gtgcctaacg atagcagcgt gccgaaaacg agcgcgctga cggtcactgc caggtaggac
     3421 ttcttggcgg acatttgcga catctactaa cccctctact acgcactcgt actagaacga
     3481 attctgagtc gggctatttg ttggcgtcgc cgtttattcg ctccgctgga cctgcatgcg
     3541 ggtgaatttc ctgtggatcg gacttgtagg tgcgttcggg gatcacatcg gagatcatga
     3601 ccgcggggct tgctgtctcg tcccccattt cggcccgcgc gccacgatct tgagcgcttt
     3661 gtcgggtaat ggcctttgcg cgttggcctc atcccaaggc gtgcgtatcc tgacgtcgcc
     3721 agcctaatat caagatcgcc gaatggcctc atcccagtgg ctctatggcg gcgacacctt
     3781 caaattatac cgctgacgga gccgctcagc gagatcgtta gtaaggtctc gccattcctg
     3841 cggattgcga atgcctccag ctgccggggt gatcgtcaac atcgctcgcc aatgcgtccc
     3901 accgtagacc gtgactcgaa atccgacctt aggacaatca gggtgtcgcg ccaggtcaag
     3961 ttcaatcatt tcggcaaggt ccgccgccgt cttcagttgt ttttgctgcg ctttaccttc
     4021 tcccgccttt gcgtgggagg cggtcggtgg cgaacttcca ctgaatgaag aatgtccctc
     4081 aacaacggag gttgcctcag cttccgaaat accgtccgta ctggagcctg gcggatttcc
     4141 tctgaggatg gcaacagtct ttccgaaaaa ttgctgtgcc aaagtgagct tgtagtcgtc
     4201 actgtggtcg tctgccatgg ccgtcgctcc tcaccgagtg tcagctcaga gaatcttcac
     4261 ttttcgctcg gcaccggtcc agacacctgc gtggcaatca gtccccgaag agagccccca
     4321 cagcatcatg aaacagtaac ccagcgatag gcatcaagaa tagcggcgta cgaaacggcc
     4381 gcgcttggcg gcgcggctca tttcatattg gaaagcagca ggtgaatgcg cgaaaggcgc
     4441 ccgtcggggg gcagctcagt actcccgcgg ctcccggcca cggactgatt ggcttccgcc
     4501 tgatcgacgg ggccaaggta gtgccccaaa tagacgccgc cgaccgcgag aacgattccc
     4561 agcacgatgg ccagcgccgt tggaattaca aaacttggac tgatcacttt gtgggtcatc
     4621 gcggatactc gctttgacct acccccacga gaaagatgcc accttccgct gagtcgggga
     4681 accagtaatt gaacggcgac attccttatt tccctttcgc cttctgcagt ccgatcttcc
     4741 tttttttcca ttcaagtcga aactccacct gtgcctcgtc aag
//
LOCUS       DATMGX010000800         2691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_211459, whole genome shotgun sequence.
ACCESSION   DATMGX010000800 DATMGX010000000
VERSION     DATMGX010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211459"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VNR65_13130"
     CDS             <1..208
                     /locus_tag="VNR65_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19661.1"
                     /translation="SGMVTVSMKASLVTSKGKVLWENPNYLYREQYQVSRDAPSFFEE
                     EEPALGRIANSFAQSLVSDILEAY"
     gene            209..1333
                     /gene="holA"
                     /locus_tag="VNR65_13135"
     CDS             209..1333
                     /gene="holA"
                     /locus_tag="VNR65_13135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWJ19662.1"
                     /translation="MRSFAASDRFVADVKARKLAPGYVFIGDETFFRKRCRDAILQHL
                     VPAEVRELSVYDMDLSEVELQHILDQARTPSLMSPFQVFFIRGVKNLYGRGKHDDEFG
                     AIEDYFKDPNPDALLIFVADHINIPTDVRRMDLTDKDRYERIRETLGEYCTVLEFARV
                     DESDAVKWAMETASGEDVKLDNDAARELVDSLGSDMMMVANELEKLVLFVGDKKRITL
                     GDVETLVLAAKQRSFYELTDAISARDRARALGVLDAILSTGDGDEAAIGHLYMLARTF
                     RQMLVILERNVRDSRALWQVLWQGFRIPPFAAEDIIRQARRYKSRRELTSAIRRVARA
                     DLALRSNPPSKRLILENLVLDLTAEPKLQEAGWLQGELPV"
     gene            1336..2127
                     /locus_tag="VNR65_13140"
     CDS             1336..2127
                     /locus_tag="VNR65_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HWJ19663.1"
                     /translation="MDVEQYLRRIEYDGPRQPSSAALRGLHRQHLFAVPFENLDIALK
                     TPICLDLALLYDKIVRRHRGGFCYELNGLFCELLIALGFRVQMLSARVRRDNGGFGPE
                     FDHMLLRVDLEEPWLVDVGFGDSFVDPIMFRAGGADQVNGHRYLVSPFGEEWQLLRED
                     DKGEVPLYVFRDVPRHLGEYEEMSAFHQTSPESGFTKRWICSKATRDGRFTVANRRLI
                     ITAADRREETVLSTDDEVRRCLRDHFGIEFDDTADLSRLDSFPNG"
     gene            complement(2226..>2691)
                     /locus_tag="VNR65_13145"
     CDS             complement(2226..>2691)
                     /locus_tag="VNR65_13145"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HWJ19664.1"
                     /translation="DKKPLSPPGEASVKFGDGKTVTIQYSRPSMRSRKIFGGLVPYDQ
                     VWRTGANSATSLKTDADLTIGGANVPAGSYTLYTLPGMKGWKLIINKQTGQWGTEYSE
                     GQDLARVDMKVAQLPSGMETFTVSLDKTSATSAMLKLDWENTIASVEVKEKK"
BASE COUNT          501 a          804 c          816 g          570 t
ORIGIN      
        1 gtcgggcatg gtgacagtct cgatgaaagc ttcgctggtc acaagtaagg gcaaggtact
       61 ctgggagaac cccaattatc tctaccgcga gcaataccaa gtctcgcgtg atgctcccag
      121 ttttttcgag gaagaagagc cggctttggg ccgcatcgcg aatagttttg cccagtcgct
      181 ggtcagcgac atcctggagg cctactaggt gcgcagtttt gccgcttcgg accgcttcgt
      241 cgccgacgtg aaggcgcgca aactggcgcc ggggtatgtc ttcatcggcg atgagacgtt
      301 tttccgcaag cgttgccgcg acgccatcct gcaacacctg gtgccggcgg aggtgcggga
      361 actcagcgtc tatgacatgg accttagcga ggtggaactg cagcacattc tcgatcaagc
      421 gcgcactcct tctctgatgt cgcccttcca ggttttcttc attcggggcg tcaagaatct
      481 ttacggccgc ggcaaacacg acgacgaatt cggcgccatc gaagactact tcaaagaccc
      541 caatcccgat gcactgctta tcttcgtggc cgatcacatc aacattccca ccgacgtgcg
      601 ccgcatggac ctgaccgata aagaccgcta cgagcgcatc cgcgagacct tgggcgagta
      661 ttgcacggtg ctcgagttcg cccgcgtgga cgaaagcgac gcggtgaagt gggcgatgga
      721 aaccgccagc ggcgaggacg tgaagctgga caacgacgcg gcgcgagagt tggtggactc
      781 tctcggcagc gacatgatga tggtcgccaa cgagctggag aagctggttc tcttcgtcgg
      841 cgacaagaag cggattacgc tgggcgacgt cgagaccctg gtgctcgctg ccaagcaacg
      901 gtcgttttac gaattgaccg acgccatctc ggcgcgcgac cgcgcccgtg ccctgggtgt
      961 actcgacgct atcctgagta ccggggacgg cgacgaggcg gccatcggcc acctgtatat
     1021 gctggcgcgc accttccgcc agatgctggt catcctggaa cggaatgtgc gcgactcgcg
     1081 cgctctgtgg caggtcttgt ggcagggctt ccgcatacct cccttcgccg cggaagacat
     1141 tatccggcag gcgcgacgct acaagtcccg ccgcgaactg actagcgcga tccggcgcgt
     1201 ggcgcgtgcc gacctcgctt tgcgctccaa tccccccagc aagcgactca tcctggagaa
     1261 ccttgtgctc gacctgaccg ccgaaccgaa gttgcaggag gctggctggc tgcaaggtga
     1321 gctgcctgtg taggcatgga tgtcgagcag tatctgcgcc gcattgaata cgacggcccg
     1381 cgtcaaccct cgtctgccgc gttgcgcggc ttgcaccgtc agcatctgtt tgcagtccct
     1441 ttcgaaaacc tcgatattgc gctgaagact cccatttgtc ttgaccttgc tttgctgtat
     1501 gacaagatcg tgcggcgtca tcgtggcggc ttctgctacg aactgaatgg gcttttttgc
     1561 gaactgctga tcgccttggg cttccgggtt caaatgctct ccgcccgggt gcgccgcgac
     1621 aatggcggct tcggccccga gttcgatcac atgctgctac gggttgactt ggaagagcca
     1681 tggttggtgg atgtcggatt cggagactcg tttgtcgatc cgatcatgtt tcgcgccggt
     1741 ggcgccgacc aggtgaatgg gcatcgctat ctggtgtcac catttggaga ggagtggcag
     1801 ttgctgcgcg aggatgacaa aggcgaggta ccgctttacg tctttcgcga cgtgccgcgc
     1861 catctcggcg agtacgagga aatgtcggcg ttccatcaaa cgtcaccaga gtccggcttc
     1921 accaaacgct ggatctgtag caaggcaaca cgggatggcc gcttcaccgt ggccaaccgg
     1981 cggctgatca tcacggccgc agaccgtcgc gaggagaccg tcctcagcac cgatgacgaa
     2041 gtgcgccgat gcctgcgcga tcatttcgga atcgagttcg acgacacggc ggacttgtcg
     2101 cgactggaca gctttccgaa tggctaggcc cgcagcgggc ggaaaaagat tcaccacaga
     2161 gacacagaga aatagaatca ggatcggccc cggatctcca ttcggctccg gcttcgtccc
     2221 gggcgctact tcttctcttt cacctccacg ctggcgatgg tgttttccca atccagcttc
     2281 aacatggccg aggtcgcgct cgttttgtcc agagagacgg tgaaagtctc catgcccgat
     2341 ggcagttgag ctaccttcat gtccacccgc gccaagtcct ggccctcgct gtattccgtg
     2401 ccccattggc cggtctgctt gttgatgatc agcttccagc ctttcatgcc gggcaaggta
     2461 taaagcgtgt agctgcctgc cgggacattg gcgccgccga tggtcaggtc ggcatcggtc
     2521 ttgaggctgg ttgcggagtt cgcgccggtg cgccagacct ggtcgtacgg cactagtccg
     2581 ccaaagatct tccggctgcg catggaagga cggctgtact gaatggtgac ggttttccca
     2641 tcgccgaact tgaccgaggc ttcgcccgga gggctcagcg gcttcttgtc t
//
LOCUS       DATMGX010000801         2450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_234776, whole genome shotgun sequence.
ACCESSION   DATMGX010000801 DATMGX010000000
VERSION     DATMGX010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234776"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VNR65_13150"
     CDS             complement(<1..751)
                     /locus_tag="VNR65_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19665.1"
                     /translation="MNIRFYRFVDRFLGPPICAALSAIDRIHSRPVAAAPPRKILVIL
                     LSEMGSMVLAQYMFAQLKQQYRGASLHVLLFARNREAVELLGLIPAENVLALEDDSFS
                     QFAWGALRVLRSLRALKVDVVIDCELFARVSSIFSYLSGAPVRVGFHRHTQEGLYRGT
                     FLNRPVLYNPYRHMSQQFLGLAAAIESCTVPAGKQVLRPIGETLPSQAIAESELRERI
                     AKLHADFPPLQNKPLILVYASGGILPIRAWPL"
     gene            complement(948..1550)
                     /locus_tag="VNR65_13155"
     CDS             complement(948..1550)
                     /locus_tag="VNR65_13155"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWJ19666.1"
                     /translation="MLPGSSGKVIIVGAHFDHISEGNGVVDNWSGASLLPSLYQALRT
                     DPRKHTYIFIGFTDEEKGEVGSRFYAQHLTKEEVVATEAMVNLDTLGLAPTEVWESHS
                     DKRLTGELVYLAKQLSLPVAGVNVDQIGSTDSVQFSERKIPSIAIHSLTQETWNAHIL
                     HTQKDKLSALRLDDYYQTYRLLAAYLVFLDQAAGAPAPAH"
     gene            complement(1578..1793)
                     /locus_tag="VNR65_13160"
     CDS             complement(1578..1793)
                     /locus_tag="VNR65_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19667.1"
                     /translation="MESLASRCNHLARALGVEALADGFTYNTIRREVVEARLQKYQGN
                     DQQREATLKQLFAEAGCDAQHLSEQPV"
     gene            1924..>2450
                     /locus_tag="VNR65_13165"
     CDS             1924..>2450
                     /locus_tag="VNR65_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HWJ19668.1"
                     /translation="MKAEIIAVGSELLTPDRIDTNSLFLTEELNKLGIEVLRKTIVGD
                     NRELLAAVFREALNRVSLVIASGGLGPTEDDLTRETVADLLGRRLQRSDEVVRAIEER
                     FRSFKRDMPAVNLRQAMVPEGAEVLNNPRGTAPGLWLEDGGRMIALLPGPPRELKPLF
                     LEEVWPRLQRRVSGVR"
BASE COUNT          535 a          688 c          732 g          495 t
ORIGIN      
        1 cgaggggcca cgcgcgaata ggcaaaatgc cgccgctcgc gtacaccagg atcaagggct
       61 tgttttggag cggggggaaa tccgcgtgca gtttagcaat tctctcccga agctccgact
      121 ccgcgatggc ttggctcggc agcgtttcgc ctatcggacg tagcacctgc ttcccggccg
      181 ggacggtgca ggactcgatt gccgccgcga gccccaggaa ctgctgtgac atgtgccggt
      241 acgggttgta aagcacgggg cgattcagga aagtgccgcg gtacaggcct tcctgcgtat
      301 gccggtggaa cccgacgcgc accggcgcgc cagacaaata agagaagatg ctgctgacgc
      361 gggcgaacag ctcgcaatca atcaccacgt ccactttcaa agcgcgcaga ctgcggagca
      421 cccgcaaggc tccccacgcg aactgagaaa aagagtcatc ctcaagggcc agcacattct
      481 ccgcagggat caggccaagc aactccacgg cttcgcgatt ccgggcaaac agcaagacgt
      541 gcaacgatgc gccgcgatat tgctgcttca gttgggcaaa catgtattga gcgagaacca
      601 tgctgcccat ctctgaaagc aaaataacca gtattttccg tggaggcgcc gctgcgaccg
      661 gccggctgtg aatgcgatca atggcagaga gggccgcgca gatgggcggg ccgaggaagc
      721 ggtcgacaaa gcgatagaat cggatattca tgcttgcgaa tcccggcggc acccgtaatc
      781 cctgtcgaga acctgtttcg ccggccctgt ggcgctccgc tacacgggag tgcgcgaatc
      841 cgccggttcc aagaatagtg cggcaaagat atcccaagag caaggaacac ttcttgcctt
      901 gggatcattt caaacaaaaa taaaacgagc ttcggcaagt cctgctccta gtgcgccggg
      961 gccggggcac ccgcagcttg atccaagaaa accagatatg cagcgagcag tcggtaggtc
     1021 tggtagtaat cgtcgagccg taaggccgat agcttgtctt tttgcgtgtg aaggatgtgg
     1081 gcgttccacg tttcttgcgt tagcgaatga atcgctatgc tggggatttt gcgttcggaa
     1141 aattgcacgg aatcggtgct gccgatttga tcgacattga cgccggctac cggcaggctt
     1201 agttgcttcg caagatagac gagctctcca gttaatcgct tatccgaatg cgattcccat
     1261 acttccgttg gcgccaggcc cagcgtgtcc aagttaacca tggcttcggt cgcaaccact
     1321 tcctctttgg tcaaatgttg cgcgtagaag cgcgagccga cctcgccctt ctcttcgtcc
     1381 gtgaacccaa taaaaatata ggtatgtttg cgcggatcag ttcttaaggc ttggtaaagc
     1441 gagggtaaca aggatgctcc gctccagttg tccaccactc cgttgccctc agaaatgtga
     1501 tcaaagtgcg ctccaacgat aatcaccttg ccggagctgc cgggcagcag gcaaacgacg
     1561 ttgggctgct tcgatgccta cactggctgt tcggaaaggt gctgggcgtc gcaaccggct
     1621 tcagcaaaca actgtttcaa ggtagcttca cgctgctggt cgttgccttg gtacttctgt
     1681 aaccgggctt cgacgacctc gcgccgaatg gtgttgtaag taaaaccatc ggcaagggcc
     1741 tctacaccga gggcgcgagc gagatgattg cagcgagagg ccagtgattc catcggtgga
     1801 ttaggtgtcg catagctaga gaataccgcc tcaggggaaa aggtagaact cttgacttgg
     1861 ggttcggttg ttaccaggtc tcgcacgggc tctcgccttc ggtgagatac aatagtcgag
     1921 gttatgaaag cggaaatcat tgccgtgggt tcggagttgc tcactcctga ccggattgac
     1981 accaactcac ttttccttac ggaagagttg aataagctgg gcattgaagt gctccgcaaa
     2041 accatcgtgg gcgacaatcg cgaattgctg gcggcagtgt ttcgcgaagc gttaaaccgc
     2101 gtttcgctgg tgatcgccag cggcggcctg ggccccacgg aagatgacct gacgcgcgaa
     2161 acggtggccg atttgctcgg acgccgcttg cagcgcagcg acgaagtggt ccgcgccatc
     2221 gaagaacgct tccgcagctt caagcgagat atgcccgccg taaatcttcg ccaggccatg
     2281 gtgccggaag gcgccgaagt gctcaacaat cctcgcggca ccgcgcccgg cttgtggttg
     2341 gaggacgggg gccgcatgat cgcgctgcta cccggcccgc cccgcgaact gaaaccgctg
     2401 ttcctggaag aagtttggcc gcgactgcag cgccgcgtct ccggcgtgcg
//
LOCUS       DATMGX010000802         3479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_429858, whole genome shotgun sequence.
ACCESSION   DATMGX010000802 DATMGX010000000
VERSION     DATMGX010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429858"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VNR65_13170"
     CDS             <1..595
                     /locus_tag="VNR65_13170"
                     /inference="COORDINATES: protein motif:HMM:NF019120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucuronidase"
                     /protein_id="HWJ19669.1"
                     /translation="TFGSDPLVVKTITAMQLNSWHVYESYTGPLGAGTLTNIVGNHYD
                     PGPESSEENGWGQWHRADHQGIGMDRTIATGTGYIAQYPPEVQKLYESLANCPDNLLL
                     FFHHVPYTYVLHSGKTVIQHVYDSHYEGADRARDFIAQWKSLAGHIDDERYSDILTRL
                     QYQAEAAIVWRDAICNWIYRLSGIPDDKGRIGSQPQK"
     gene            667..2313
                     /locus_tag="VNR65_13175"
     CDS             667..2313
                     /locus_tag="VNR65_13175"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HWJ19670.1"
                     /translation="MNRFSRSRRTFLKQASAAAIAGSAGVLGSRPLAAFAQSKSPSDP
                     AAPARVFIDSRRSIAPLDRNLFGSFLEHLGRAIYGGIYDPGSKLSDSNGLRKDVLNEI
                     RQLGIPIVRYPGGNFVSGYHWLDGVGPKQDRPRVLDKAWNSIETNQFGTNEFMAWCQA
                     VGALPLMGLNLGTGTPEQAAALVEYCNVEKGTKWSDLRRQHGIAEPYGARYWCLGNEM
                     DGPWQIGHMSATEYGVKAADAAREMRSVDPSLKLVACGSSGPFMPTYLEWDREVLEQC
                     YEYVDGLSLHRYFGNSQSDTGGDSAKFLAMNLSMERQIAETAAVCDLVRGHKRSPKKL
                     WLSFDEWNVWYRANSGDAVDGHRKEAPPLLEEVYNLEDALLVGGLINSLVRNADRVKI
                     ACLAQLVNVIAPIMTNAGGLFRQTIFYPYSWALQYAKGAVLNLLVESAGYSVPGFDNV
                     PYVDVAGTLDESAGTVSLFVLNRDLAKSRIVEVQWQDHFPSKVLASWLLTGDDLKASN
                     SFAAPQRVAPQAFAAPSTKGSRSVLELPARSYAAMQWSLA"
     gene            2479..>3479
                     /locus_tag="VNR65_13180"
     CDS             2479..>3479
                     /locus_tag="VNR65_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWJ19671.1"
                     /translation="MGKAPTTLRDIAKALGTSIGSVHRALHDNPGVSPLTKDRVLQMA
                     RTLGYRPNLAARYLSSKKNLRISVNTLQGTTSFWDEVRTGIREAASSILLENVEVEFR
                     TYPRLGEGEEEAFEAAINDKVDGIISFPSRPQVLRTWIRRASRGRIPVVCVATDAPNS
                     GRLGIVAIDTLASGSIAADLLGRFLGPKEGSIAITLFEMAITEHAEKYAAFENTLRSF
                     YPNLRLLKPIEDHDVETEAYSKCRKLFEKCPDLAGIYVSTEASIPVLNAARDANMLDR
                     LTIITTDLFPDLVPQIRSGAVAATIYQRPRAQGEIAFRMLHEFLVEGVSRSHQVALAP
                     "
BASE COUNT          726 a         1019 c          994 g          740 t
ORIGIN      
        1 cacttttggc agcgacccgc tcgtggtgaa gaccatcacc gcgatgcaac tcaactcgtg
       61 gcacgtgtac gagagttaca ccggtccgct gggcgcggga accctcacca acattgtcgg
      121 aaatcattac gatcccgggc cggagtcttc ggaagaaaac ggctggggac aatggcaccg
      181 cgccgatcat cagggaatcg gcatggatcg caccatcgcg accggcaccg gatacatagc
      241 tcagtatccg ccggaggtac aaaagctgta cgaatcgctg gccaactgtc ctgacaacct
      301 gttgttattt ttccatcacg tgccctatac ctacgtcctg cattccggca agaccgtaat
      361 tcagcacgtg tacgattcac actatgaagg cgcagatcgg gcccgcgatt tcatcgcgca
      421 atggaagtcg ctcgcagggc acatcgacga tgagcgttat agcgatatcc tgacgcgcct
      481 gcaatatcag gcggaagctg ccatcgtgtg gcgcgacgcc atctgcaact ggatctatcg
      541 cctctcgggc attccagacg acaagggacg cattggtagt cagcctcaga agtagctccc
      601 aagtctgagc cgagttttgc aaacaattcg atgtgcgtta gattggactc cgattggagc
      661 tcgcgcatga atcgcttttc tcgttccaga cggacttttc tgaagcaggc ctcggccgct
      721 gccatcgccg gttccgcggg tgtactcgga tcgcgaccgc tggctgcctt tgcgcaatcg
      781 aaatcgccat cggaccctgc tgcgccagca cgagtcttca tcgacagccg aagaagcatc
      841 gcgccgcttg atcgcaatct cttcggctct tttctcgagc atctcggtcg tgccatctac
      901 ggcggcattt acgatcccgg ctcgaagctt tccgattcga acggccttcg caaagatgtc
      961 ctcaacgaga tccgccagct cggcattccc atcgtccgtt atcccggagg caatttcgtt
     1021 tcgggttatc actggctcga tggcgtcggt ccgaagcagg atcgtccccg cgtgcttgac
     1081 aaagcctgga actcgatcga gaccaaccag ttcggcacca atgaattcat ggcgtggtgc
     1141 caggccgttg gcgcactccc tttgatggga ctcaacctcg gcaccggcac tcccgaacag
     1201 gctgccgcgc ttgtcgaata ttgcaacgtg gagaagggaa ccaagtggag cgacctgcgg
     1261 cggcagcacg gcatcgccga accatatggg gcgcgatact ggtgtctcgg caacgaaatg
     1321 gacggccctt ggcagatcgg ccacatgtcc gccaccgaat atggagtcaa ggccgccgat
     1381 gctgcccgcg aaatgcgcag tgtggatccc tctctcaagc tggtggcgtg cggatcgagt
     1441 ggccccttta tgccgaccta cctcgaatgg gatcgcgagg tcctggagca gtgttacgaa
     1501 tatgtcgacg gactctcgct gcatcgctat tttggcaaca gccagagcga caccggcggg
     1561 gattccgcaa aatttctggc gatgaacctg agcatggagc ggcagattgc cgaaaccgca
     1621 gcggtctgcg atctggtgcg aggtcacaag cgatcgccga aaaaactgtg gctttcgttt
     1681 gacgaatgga acgtgtggta ccgcgccaat agcggcgatg ccgttgacgg ccacagaaaa
     1741 gaagcaccgc cgctgctcga agaggtctac aacctggaag atgcattgct cgttggcgga
     1801 ctcatcaatt ctctggtacg caacgctgat cgcgttaaga tcgcgtgcct ggcgcagttg
     1861 gtgaacgtga tcgctccgat catgacgaac gcgggcggcc tgtttcgcca gacgatcttc
     1921 tacccgtata gctgggctct gcaatatgcg aagggcgcgg tgctgaatct tcttgtggag
     1981 tccgcgggct actcagttcc gggcttcgac aacgtcccgt atgttgacgt ggccggaaca
     2041 ttggatgaaa gcgcaggaac agtctcgctc tttgttctta accgcgacct ggcaaaatct
     2101 cgtatcgttg aggtgcaatg gcaagatcac ttcccgtcaa aggtattggc ttcatggctg
     2161 ctcacgggcg atgatctaaa agcctcgaac agttttgctg cgccgcaacg tgtggcgccg
     2221 caggctttcg cagcgccttc gaccaaggga tcgcgtagcg tcctggaatt gcccgcacgt
     2281 tcttacgcgg cgatgcagtg gtcgctggcc tgacctcggg ttcaccgaac tctcggcgcc
     2341 attttaggat ttattttgcg gacgccttgc ggatttggaa gacttccgtt aacgtttacg
     2401 gttccggcga ccgggcagcc ctcccccagg ctattctgat ggacctccgg tcgccggcag
     2461 gaacacgggc caggaaaaat gggcaaagct cccacgactc tccgtgacat tgcgaaggcc
     2521 ctgggcacct cgatcggatc ggtgcatcga gcgctgcatg acaatccagg ggtaagtcca
     2581 ctcaccaaag acagagtctt gcagatggcg agaactctgg gataccggcc aaatcttgcg
     2641 gcccggtatt tgtcttccaa gaagaatctg cgaatctcgg tcaacactct gcagggaacc
     2701 acatcgtttt gggacgaggt gcggaccggc atccgcgaag ccgccagttc gattctgctc
     2761 gaaaatgtcg aggtcgagtt tcgaacctat ccgcgtctcg gcgaaggaga agaagaagct
     2821 tttgaagcgg ccatcaacga caaggtcgac ggcatcatca gcttccccag ccgtccgcag
     2881 gttttgcgga cctggatacg ccgtgcctct cgcggccgga ttcctgttgt gtgtgtcgcc
     2941 actgatgctc ccaacagtgg ccgcttgggc atcgttgcga tcgacacgct ggccagcggt
     3001 tccatcgctg ccgatctcct aggcagattt cttggcccaa aggagggatc gatcgcgatc
     3061 accttgtttg agatggccat caccgagcac gcggaaaaat atgctgcatt tgaaaacacg
     3121 ctgcgatcct tctatcccaa ccttcgcctg ctaaagccca tcgaagatca tgatgtggag
     3181 acggaagcgt acagcaaatg ccgcaagctg tttgaaaaat gtcctgactt ggccggaatc
     3241 tacgtttcga cggaggcctc gatccccgtg ctgaacgcgg cccgtgacgc aaacatgctg
     3301 gatcgtctga ccatcatcac gacggatctg tttcccgacc tcgttcctca gatacgatcg
     3361 ggcgcggtag ctgcgaccat ttaccagcgt ccgcgcgcgc agggcgagat cgccttccgc
     3421 atgttgcacg agttcctggt ggagggcgtc agtcgatcgc atcaggtcgc gctggctcc
//
LOCUS       DATMGX010000803         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_262909, whole genome shotgun sequence.
ACCESSION   DATMGX010000803 DATMGX010000000
VERSION     DATMGX010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262909"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VNR65_13185"
     CDS             complement(<1..780)
                     /locus_tag="VNR65_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWJ19672.1"
                     /translation="MPGFPIPANERARLRELQSLRFEEWGSRGALDDLCAVTARLLDT
                     PIAGLSLVDRNEQLFAGKTGLGADRISRAIAFCPHTIMTPAPFIVEDAANDPLFCQNP
                     LVTECPNVRAYLGIPLETTPGLRVGALCAVDQKPRHFTDGDIQTLTKLAGIAVSMLKS
                     HRATLELDDQLTGAVALQEKMLPGKTRIEHIQAACPLDVASYYIARDGIGGDIWGIES
                     TGPQRVLLYVADFTGHGLAAALNTARFHSFVHIASQRTDKPA"
     gene            complement(969..1514)
                     /gene="hpt"
                     /locus_tag="VNR65_13190"
     CDS             complement(969..1514)
                     /gene="hpt"
                     /locus_tag="VNR65_13190"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWJ19673.1"
                     /translation="MPIVRGRNIEVLFSADMIARRNLELAKEIATHEFHDLLVIAILK
                     GSFVFAADLIRCMHGAGLAPEVEFILISSYGAGKESGGVRILRDIDASVKGRDVLLID
                     DILESGNTLAYTRYLALNRGARSVSIAVLLDKRGRRQLDIEAQFVGFACPDRFVVGYG
                     MDVAHTFRELPFVGVITGEDD"
     gene            1688..>2136
                     /locus_tag="VNR65_13195"
     CDS             1688..>2136
                     /locus_tag="VNR65_13195"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HWJ19674.1"
                     /translation="MRLLVVGAGSTGGYFGGRLAQAGRDVTFLVRAARADQLKAKGLH
                     IVSPHGDVTLAPKLVTRDAIDAPYDAILLTVKAYSLDGALKDIAPAVGPNSMIVPMLN
                     GMRHLESIAAAFGRDALVGGLCRIAAALDEQGGIVHLSHFHDLAYGE"
BASE COUNT          445 a          622 c          617 g          452 t
ORIGIN      
        1 tgccggcttg tccgtgcgct ggctcgcgat atgcacgaag gagtgaaacc gggccgtgtt
       61 caacgcagcg gcaagaccat gacccgtgaa atcggcgacg tacagcaaaa cgcgctgggg
      121 gcctgtggat tcgattcccc aaatatcgcc gccgatgccg tcgcgcgcta tatagtagct
      181 tgccacgtct aaaggacagg ccgcctgaat gtgctcgatc cgcgtctttc ccggcagcat
      241 cttttcttga agcgcgaccg cgcccgtcaa ttgatcgtcg agctcaagcg tcgcgcggtg
      301 gctcttaagc atcgacacgg caatgccggc aagctttgtc agggtttgga tgtcgccatc
      361 cgtgaagtgg cgtggttttt gatccacggc gcacaaggcg ccgacgcgaa ggccgggcgt
      421 tgtttcaagc ggaatgccaa gataggcccg gacgtttgga cactcggtta cgagcgggtt
      481 ttggcaaaag agcggatcgt ttgcggcatc ctcgacgatg aatggcgccg gggtcatgat
      541 ggtgtgcggg cagaacgcga tcgccctcga tatccgatcc gctccaaggc cggtcttgcc
      601 ggcaaagagc tgttcgttgc ggtcgacaag cgagagaccg gcgatgggcg tatcgagaag
      661 ccgtgcggtg acggcgcaaa gatcgtcgag cgctcctctc gagccccatt cctcaaaacg
      721 caggctttgt agctctctca ggcgcgccct ttcattggcg ggaatcggaa agcctggcat
      781 gtcgctctct ccttgaccct tgcccgggca gcgcttcatg cgcttcctca gcactatgca
      841 cgcattcggt tcatcgcctg tttcacgatc ggctaaaatt cgaacgtctt gagcgagacc
      901 ccgtgcgcgc acgtcctttt tggcactgac tgcggcgaat tcatcaggag gttgagatct
      961 acgtggcgct agtcgtcttc tcccgtgatc acgcccacga agggaagttc gcgaaaggtg
     1021 tgagcaacgt ccatcccgta gccgacaacg aagcgatcgg gacacgcaaa tcccacgaac
     1081 tgtgcctcta tatccagctg gcggcggcca cgcttatcca ggagcacggc aatcgaaacg
     1141 cttctcgctc cccggttcag agctagatag cgcgtgtatg ccagcgtgtt gccggattcc
     1201 agaatgtcgt cgatgagaag gacatcccgg cccttcacag aggcatcgat atcccggaga
     1261 attctgacgc cgccgctttc ctttcccgcc ccatagcttg atatgagaat gaactccacc
     1321 tccggtgcga gcccggcgcc gtgcatgcag cggatgagat cggcagcgaa gacaaaggag
     1381 cccttcagga tcgcaataac gagaagatcg tggaattcat gcgtcgcgat ttcctttgca
     1441 agctcgagat tccggcgcgc gatcatgtct gcggaaaaga gcacctctat atttctgcct
     1501 cggacaatcg gcatcgctca agctccaatg gcggtcgagc ataatcttaa ttgaaagtta
     1561 tatgccggcc gggttgcccc aagatttcgt ctcccataaa ggtcgcgaag ccaatagaca
     1621 attttagtct ttccattgtg acatcataac cccaggtaat agcttaaaac tgagggattt
     1681 tcggacaatg cgcttattgg tcgtgggagc aggttcaacc ggcggatatt tcggaggacg
     1741 cctcgcccag gccgggcgcg acgtcacctt tctggtgcgc gctgcccgcg cggatcagct
     1801 gaaggcgaag gggttgcata tcgtcagccc tcacggtgac gtcacgctcg caccgaaact
     1861 tgtcacaaga gatgcgatag acgcgccata cgacgcaatc ctgcttacgg taaaggccta
     1921 ctctcttgac ggagccctga aagatattgc tcccgccgtc gggccgaaca gcatgatcgt
     1981 gccgatgctg aacggcatga gacacctcga aagcatcgcc gcggctttcg gccgggacgc
     2041 gctcgtcggc ggtctttgca gaatagccgc cgcgctcgac gagcagggag gtatcgttca
     2101 cctctcccac tttcatgatc ttgcttatgg cgaatt
//
LOCUS       DATMGX010000804         2156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_203098, whole genome shotgun sequence.
ACCESSION   DATMGX010000804 DATMGX010000000
VERSION     DATMGX010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203098"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VNR65_13200"
     CDS             complement(<1..287)
                     /locus_tag="VNR65_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19675.1"
                     /translation="MSNLFVFGFGYSAQAVAAILQTGRWHVSGTVRSADKTSELARDG
                     VSAILFGDRAAVEHALQTATHLLVSTPPGSTGDPALTTYRQAVRDAPALKW"
     gene            complement(284..1441)
                     /gene="queG"
                     /locus_tag="VNR65_13205"
     CDS             complement(284..1441)
                     /gene="queG"
                     /locus_tag="VNR65_13205"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWJ19676.1"
                     /translation="MQTSISEGEAASTLQPELTDWLKERVLAEGFEAVGIASPSAVRN
                     ASRNLFAFLEAGFHGDMGWLATNADRRADPRVLWPDVRSIVMAGMNYAPEADPLAATK
                     RPSHAAISVYAQGADYHDVIKGKLKAIAADFAHRTSEDVKVFVDTAPVMEKPLAEATG
                     IGWQGKHTNLVSRDFGSWLFLAAIFTTAELTPDAPAAGNCGSCNRCLDACPTRAFPAP
                     YRLDARRCISYLTIEHKGQIPHEFRAAMGNRIYGCDDCLAVCPWNKFAEAAREMRFRP
                     GEGAHNPPLAELLQLDDAQFRARFRGSPIKRVGRDRFVRNVLVAAGNSGNPALLHDIM
                     LLLSDTSPLVRGMAVWASRRLADADREAELKERYASKESDVLVCAEWDRTP"
     gene            complement(1422..2111)
                     /locus_tag="VNR65_13210"
     CDS             complement(1422..2111)
                     /locus_tag="VNR65_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWJ19677.1"
                     /translation="MANLLHYPLCPASRAVRLALVESGIDPTFSEVKPWAPTLDFLNI
                     NPAGTLPVLMIEGKTLCGAYPIVEYLSETVTRDGLPSARSALWTGTPSERAEERRVAE
                     WFLRKFDAEVTQTILEEKFYKPMSRGRLAPDLGTIRTGRSNLRYHLSYVSFLSDQRKW
                     LGGDHLSFADLAAAAQMSVMDYLDEMPWADFPEAKALYQRLKSRPSFRPLLQDRIPGF
                     SPPEHYANLDF"
BASE COUNT          448 a          653 c          646 g          409 t
ORIGIN      
        1 atccatttca gcgccggggc gtcgcgtacc gcttgcctgt aggtggtcag cgccggatcc
       61 ccagtgctgc ccggcggcgt tgacacgaga agatgggttg cagtctgaag cgcatgctcc
      121 accgcggcgc ggtcgccgaa cagaatggct gaaacgccgt ccctcgcaag ttcgctcgtc
      181 ttgtccgccg agcggacggt ccccgaaacg tgccatctgc cagtttgaag tattgcggcc
      241 acggcctgcg ccgagtatcc gaatccgaaa acgaaaagat tgctcatggc gtcctatccc
      301 attccgcaca caccagaacg tcgctctctt tagaagcgta tctctctttc agctccgcct
      361 cgcgatcagc gtctgcaagc cggcggctcg cccagacggc catgcctcgc accaaaggcg
      421 aagtatcgct caggaggagc atgatgtcat ggagaagagc cggattgcca gaattgcctg
      481 cagcgacgag aacatttcga acgaagcggt cgcgcccgac ccgcttaatt ggcgaacccc
      541 tgaaccgcgc gcggaattgg gcgtcgtcaa gctgcaacaa ctcagcgagc gggggattat
      601 gggcgccttc gccgggtctg aacctcatct cgcgggccgc ttcagcgaat ttgttccatg
      661 ggcagactgc tagacaatcg tcgcaaccgt aaatgcggtt tcccatcgca gcgcgaaact
      721 catgcgggat ctgccccttg tgctctatcg tgagatagga aatgcagcgg cgtgcgtcga
      781 gccggtacgg ggctgggaaa gcgcgcgtcg gacaggcatc gaggcaccgg ttgcaggacc
      841 cgcaattgcc ggccgccggc gcgtccggcg tcagttccgc agttgtgaaa atcgccgcga
      901 ggaacagcca cgatccgaag tcacgcgaga caaggttggt gtgtttgccc tgccagccaa
      961 tcccggtcgc ctcagcgagc ggtttctcca tgacgggggc ggtgtcgacg aacaccttga
     1021 cgtcctcgct cgtccggtgc gcgaagtctg ccgcgatcgc tttcagtttg cccttgatga
     1081 cgtcgtggta gtccgcgccc tgggcgtaga cggagatggc tgcatgagat ggtctcttgg
     1141 tcgccgcgag cgggtcggcc tcaggcgcat agttcatgcc tgccatcaca atcgagcgca
     1201 cgtcaggcca aagcactcga ggatctgccc ggcggtcggc gttcgtcgcg agccatccca
     1261 tatcgccatg aaacccggct tcgagaaagg caaacaggtt ccgagaggca tttcggacgg
     1321 cggacggcga tgcaattcca accgcttcga agccttccgc gagaactcgc tctttgagcc
     1381 agtccgtcaa ctcgggctga agagtggaag cggcttctcc ctcagaaatc gaggtttgca
     1441 taatgctccg gcgggctgaa acccggtatg cggtcttgaa gcagcggacg gaaggacggt
     1501 ctcgatttca ggcgttgata caaggctttt gcctcaggaa aatcagccca aggcatctca
     1561 tcgaggtaat ccatcaccga catctgtgca gcggcggcca aatccgcaaa gctcaaatga
     1621 tcgccgccga gccattttcg ctgatcgctc agaaaactca cataagaaag atgatagcgc
     1681 agattgcttc tgccagtcct tatcgtgcca aggtcaggcg ctaggcggcc gcgagacatt
     1741 ggcttgtaaa acttttcctc gaggatggtt tgtgtcacct ccgcatcgaa cttgcgcagg
     1801 aaccattcgg ctacgcgccg ctcctcggcg cgttcggagg gcgtccccgt ccaaagggcc
     1861 gaccgcgcgg aaggcagccc gtctcgcgtg accgtctccg acagatactc cacaataggg
     1921 taggctccgc acagggtctt accctcgatc atcagaacgg gcagcgttcc cgccggattg
     1981 atgttgagaa aatccagcgt gggcgcccat ggtttgacct ctgaaaaagt cgggtcgatc
     2041 ccgctctcga caagagcaag ccttacggcg cgagacgcag ggcaaagcgg gtaatggagg
     2101 agatttgcca tcgatcaata cccgccaaaa cgcgcagcgc taaagaacgc tggcga
//
LOCUS       DATMGX010000805         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_232239, whole genome shotgun sequence.
ACCESSION   DATMGX010000805 DATMGX010000000
VERSION     DATMGX010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232239"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..687
                     /locus_tag="VNR65_13215"
     CDS             289..687
                     /locus_tag="VNR65_13215"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWJ19678.1"
                     /translation="MTTDRKEPSYVFSEKQVAEKSELARHFLENRTRLNENVGQTDNL
                     LVKRFYNLDHNTYLEGALPAKYKELMGLVASASLRCDDCINFHVIQSYRLGVTRAEQE
                     EAINIALIVGGSIVIPHMRRAYELMTELYG"
     gene            complement(776..1957)
                     /locus_tag="VNR65_13220"
     CDS             complement(776..1957)
                     /locus_tag="VNR65_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ19679.1"
                     /translation="MPAIKFRGVDFIDFDSLLSDDERLVRDTARKFIEDNLVPIIEEC
                     NRAGRFPRELVKPMAGLGFFGASLNGYGCAGMSNVEYGLVTQELERGDSGVRSFVSVQ
                     SALVMYPIYAFGSDEQKNTWLPAMQKGEKLGCFGLTEPDFGSNPGGMRTHAKKIGKEY
                     VLNGEKMWITSGTIADVAVIWAKVEDEGNRVRGFLVETDRPGFKADDIHGKWSLRASV
                     TSGLSLQDVRIPESNLLPGTGGLKSPLMCLNQARYGIAWGAVGAAMSCYDCALQYSLL
                     RKQFRDQPIASHQLVQEKLAWMISEITKAQLLVLQVGRLKDAGKVQHQHISMAKRNNV
                     WMALECARLSRDILGANGVADDYPIMRHLMNLESVKTYEGTHDIHTLIIGASVTGIDA
                     F"
     gene            complement(2115..>2317)
                     /locus_tag="VNR65_13225"
     CDS             complement(2115..>2317)
                     /locus_tag="VNR65_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19680.1"
                     /translation="SKQIIVLREAVSRAIAIKGRCFFVSPNTFQIFSSISLTLSNAAE
                     AVWAEYERYRESVPCWMPNSRR"
BASE COUNT          526 a          689 c          617 g          485 t
ORIGIN      
        1 gttctccacc acgcgcaaaa attccagcgc cagatcgctt tcaatatttt ccccaaaatt
       61 cttggcatgt ggttcggtac gggtttgggt tggcatcggg gcctcctgta tataaaacgc
      121 gaccttggaa tatattccct tcggagccaa gagggaagta gtgtggcgcg ggccccccgc
      181 ccgcgaacgc gtccagagag atggaccggc gacaaccccg gatgttgctc gtgccgcaag
      241 gccacagttg gcatcctcaa cggcaaaacc gtctgctaat cttcacccat gaccaccgac
      301 cgcaaagaac caagctacgt cttctccgag aagcaggttg ccgaaaaaag cgaacttgcc
      361 cgccatttcc tggagaaccg cacccgtctg aacgaaaacg tcggccaaac cgacaacctc
      421 ctcgtcaaac gcttctacaa cctggatcac aacacttatc tcgaaggcgc tctgcccgcg
      481 aaatacaaag aattgatggg gctggttgcg tcagcatctt tgcgttgcga cgactgcatc
      541 aacttccatg taattcagtc ctaccgcctg ggcgtgacgc gcgccgagca ggaggaagcg
      601 atcaacatag cgctgatcgt tggcgggagc atcgtgatac cgcacatgcg gcgagcgtat
      661 gaactgatga cggagctgta cgggtgagtc ccaaccgctg ctgcgtgcgt gaccggatcg
      721 cggagacgat cgttgtcagc ggcaagaaat accaagcgaa gcttgaggct ggatctcaga
      781 acgcgtcgat cccggtcacg ctcgccccga tgatcagcgt gtggatgtca tgcgtgccct
      841 cataagtctt caccgattcc agattcatca gatggcgcat gatggggtag tcgtcagcca
      901 caccgttcgc ccccagaata tcgcgggaga gacgcgcgca ctcgagcgcc atccagacgt
      961 tatttcgttt tgccattgag atgtgctggt gctgtacctt tccggcgtct ttcaagcggc
     1021 cgacttgcag cacgagcaac tgtgctttcg tgatctcgct gatcatccag gcgagttttt
     1081 cctgcacgag ttgatgcgag gcgatgggct gatcgcgaaa ctgtttgcgc aacaacgaat
     1141 actgcagcgc gcagtcgtaa cacgacatcg ccgcgccgac ggcgccccac gcgatgccat
     1201 agcgcgcctg gttcaggcac atcagcggag acttcaatcc gcccgttccc ggcagcaggt
     1261 tcgactcggg aatccgcaca tcttgcagcg acaagcccga cgtcaccgac gcgcgcagtg
     1321 accacttccc atgaatgtca tcggccttga atccaggacg gtcggtctcg acgagaaatc
     1381 ctcgtactcg attgccttcg tcctcgacct tggcccagat caccgccacg tcggcgatcg
     1441 tgcccgacgt gatccacatt ttttcgccgt tcaacacata ctctttgcca attttcttcg
     1501 cgtgcgtcct catgccgccc ggattcgatc cgaagtctgg ttcggtcagt ccgaagcaac
     1561 cgagcttttc gcctttttgc atcgcgggca gccaagtgtt cttctgttcg tcgctgccga
     1621 aggcatagat tggatacatc accagcgccg actgcacgct gacgaacgag cgcacgccgg
     1681 aatccccgcg ttccagttct tgcgtgacca gaccgtattc gacgttcgac attccggcgc
     1741 agccgtagcc gttcagagaa gcgccgaaga atcccagacc ggccatcggc ttcaccaatt
     1801 cgcgtgggaa gcggccggcg cggttgcact cctcaatgat cgggacgagg ttgtcttcga
     1861 tgaacttgcg agcggtgtcg cgcaccagtc gctcgtcgtc gcttagtaac gagtcgaagt
     1921 cgatgaagtc cacaccacgg aatttgatgg ccggcatttt acgtttcacc ccacaaagtc
     1981 agcagactgc gaacaggaaa ctaatcaaag tagcagagcg gcggggaagt cagcaatgcg
     2041 ggttgggggc gaaccgcaaa atcttcaaac cctcagcgtc atagacaaaa tttagaagac
     2101 ggcagcaaga gatcctagcg ccttgagttt ggcatccagc agggtaccga ttcgcgatag
     2161 cgttcgtact ctgcccaaac tgcttccgca gcgttggaca atgtaagaga tatcgacgaa
     2221 aatatttgaa aagtattagg tgaaacaaaa aagcagcgcc cttttattgc aatcgctctc
     2281 gacaccgctt cccgcaagac gataatctgt ttcgacc
//
LOCUS       DATMGX010000806         3017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_841,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000806 DATMGX010000000
VERSION     DATMGX010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VNR65_13230"
     CDS             complement(<1..1017)
                     /locus_tag="VNR65_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19681.1"
                     /translation="MLSLASLFVITAAAGISVRALGGSRTAAAFGAFWLFATLTQFFQ
                     YYVGVNDPSLLAIALMALGLAYFLYCLQAGRAVEPAIALMVIAGFFKHNFPMIPAVAL
                     IWLTFIDKRAALRATVFGALLCIIGVLHCIMAFGPNFLNEMMMPREITFNHILSAANR
                     LQWIAPAVAAWGLWAWPNRRQPAAQFTALLIVMTLFNGLYTAAGAGVTYNAYLAVTFA
                     TAIAIGVAIERIDATKIWRRLTPSAVKIIIVSLLLLRVVLWPRYEYYLVLASPSFREE
                     YRQDSIVLNNEIARIRAIQGPVACEPLIICYRAGKAFVYERYWVEQLIATGRWTKDAI
                     DKGVR"
     gene            complement(1628..2758)
                     /gene="modC"
                     /locus_tag="VNR65_13235"
     CDS             complement(1628..2758)
                     /gene="modC"
                     /locus_tag="VNR65_13235"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HWJ19682.1"
                     /translation="MIEVSAFQRLGGFTLDATFAAESRFIALFGQSGSGKTTLLNVVA
                     GLLRPERGRVIVDGTTLFDSDRGIFLPPYRRQLGAVFQEGRLFPHLSVRQNLLYGAWF
                     ARLSGKEAKLARVADLLGIEPLLTRYPNRLSGGEKQRVAIGRAILASPKLLLMDEPLA
                     SLDDPRKQEIMPYLERLRDEARIPIIYVSHSVAEVARLSDTLVILAGGKVRAAGPTIE
                     LMQRLDLLPVIDGGGTGAVIEAAVESHDDIYGLTTLVSRAGQWRVPRLEIAPGKRVRM
                     QIKASDVMIALRQPDDMSALNVIPAVVAEIGPLDSPGVDIKLDCGGEALVARLTRYSA
                     ERLSLRTGTPVFAIVKSVSFDRAALGAAWSDIRAADAAAGSQ"
     gene            complement(2755..>3017)
                     /locus_tag="VNR65_13240"
     CDS             complement(2755..>3017)
                     /locus_tag="VNR65_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HWJ19683.1"
                     /translation="LPGIIAGMILSFARSMGEFGATITFVSNIPGETQTLPSAIYTLT
                     QVPGGDAGALRLTVISIFISMAALLLSEFLARRAGRSLHVAT"
BASE COUNT          661 a          867 c          934 g          555 t
ORIGIN      
        1 tcggacgcct ttatcgatcg catccttggt ccacctgccc gttgcaatga gctgctcgac
       61 ccaatagcgt tcatagacga aggcttttcc tgcgcgataa cagatgatta gaggttcgca
      121 tgcgacaggg ccttgtatcg cgcggatgcg cgcgatctcg ttattcagca cgatggaatc
      181 ctgacggtat tcttcccgga acgatggact tgcgagcaca aggtagtatt catatcttgg
      241 ccacaagacg accctaagca gcagcaggga tacaatgatt atttttacag cactcggggt
      301 caagcgccgc catatttttg tggcgtcgat tctttcaata gctactccta tcgcgatggc
      361 ggtcgcaaag gtcactgcca aatatgcgtt ataggtgacg ccggcgccgg ccgcggtata
      421 aagcccgttg aagagcgtca tcacaatcaa gagtgcagta aattgcgctg ccggctgcct
      481 ccggttcggc caagcccaga gcccccaagc agccaccgcc ggtgctatcc attgcagcct
      541 gttggcagcc gataaaatat gattgaaggt tatttcgcgc ggcatcatca tttcattgag
      601 aaaattaggg ccgaacgcca taatgcagtg caaaacccca atgatacaaa ggagtgctcc
      661 aaatactgtt gcgcgcagcg ccgctctttt gtctatgaac gtcagccaga taagcgcgac
      721 ggctggaatc attggaaaat tatgcttgaa gaagccggcg attaccatca gcgcaatggc
      781 cggctctacg gcgcgccctg cctgcaagca gtacagaaaa tacgcgagcc ccagggccat
      841 cagggctatc gctaacaagc tcggatcgtt gacgcccaca tagtattgaa agaactgtgt
      901 cagggttgca aacaaccaga aagcgccgaa agcggccgca gtgcgcgacc cgcccagcgc
      961 acgcaccgaa atgcccgctg ctgctgtgat gacgaacagc gaggcgagcg agagcaaccg
     1021 tccggcgagg atagcgtcgc ccgtcagcct cgcgactgcc gccgtgaggt agaacgagag
     1081 gggcggatag ttgttgacgc gcatctcatc cgggccaggg taaagcggca agccttgcgt
     1141 tatcgcgtcg atataccagg cgtgccagcc ctcgtttctg ttggcgtcga ggggaaagcc
     1201 gatgcgccag aggggccaca caaggtagat cgcgccgaga atggcgaaga tccacgtagc
     1261 gatttttacc gcggaaagac gggaggctgg cgccttagcg atcgagtcgg ggagcgaggc
     1321 ggaagagaaa gacatgataa gcttttgtga gagctgagaa ttgatgcgcg tagaggattg
     1381 aggaattaag tccgaagcgt ggcaataacg gcgtgtatcc gtcgttcggg cagccgccac
     1441 tgtggcgagt tgtgcataaa agcatggctt ggcaatgagt tctctcacga cacctgtccc
     1501 gtttctagag ccaaacgcgc gaggtgattt cgggtgaatc cgagtcggcg cgccaaaaat
     1561 tgcaataatc cgacgtgccg ctctcagcct attgggtggg ttcttggcga ggccggagca
     1621 accctgctta ttgcgatccg gctgccgcat ccgccgcccg gatgtcgctc caagccgcgc
     1681 cgagggcggc acggtcgaag gaaaccgact tcacgatggc aaaaacgggc gtccccgtgc
     1741 gcaaggaaag ccgctcggcg gagtagcgcg tgagccgcgc caccagcgcc tcgccgccgc
     1801 aatcgagctt gatgtccacg ccagggctat cgagcgggcc aatctccgca accacggcgg
     1861 ggatgacgtt cagggcgctc atgtcgtcag gctgacgcaa tgcgatcatc acatcgctgg
     1921 ccttgatctg catccgcaca cgctttcccg gcgcaatttc gagccgcggg actctccatt
     1981 ggcctgcgcg cgagacaagc gtggtcaggc cgtaaatgtc gtcgtggctt tctacagcgg
     2041 cctcaatcac cgcccctgtt cccccgccgt cgatcacggg cagaagatcg aggcgttgca
     2101 tcagctcgat cgtcggaccg gctgcgcgca ccttgccgcc cgcgaggatc accagcgtat
     2161 cggacagccg cgcaacctct gcaacggagt ggctgacata gatgatgggg atgcgggcct
     2221 cgtcgcgcag gcgttcgaga tagggcatga tctcctgctt gcgcggatcg tcgagcgagg
     2281 caagcggctc gtccatcagg agaagtttcg gcgaggcgag gatcgcgcgt ccaatggcga
     2341 cgcgctgctt ctcaccgccc gagagccggt tcggatagcg ggtcagcaga ggctcgatgc
     2401 cgagcaagtc agcgacgcga gcgagcttcg cttccttgcc ggacaggcgt gcgaaccatg
     2461 cgccgtagag cagattctga cgcacgctca gatgcgggaa gaggcgccct tcctgaaaca
     2521 cggcgccaag ctgacgccga tagggtggca ggaatatccc gcgatccgaa tcgaaaagcg
     2581 tggtgccatc aacgattacg cgtccgcgct cgggccgcag caatcctgca acgacgttga
     2641 ggagcgttgt tttgccggag cctgactgtc cgaacagcgc gatgaagcgg ctctccgccg
     2701 cgaacgtggc gtcgagcgtg aaaccgccga gcctctgaaa agcgctgact tcgatcatgt
     2761 tgcgacgtgg aggctgcggc cggcgcggcg cgcgagaaac tcggacaaca atagtgccgc
     2821 catcgagatg aagatggaga tgactgtaag ccgcagcgcg cccgcgtcgc cgccgggcac
     2881 ttgcgtcagt gtgtagatcg cggacggcag cgtctgggtt tcgcctggaa tgttcgaaac
     2941 gaaggtgatg gtcgcaccga actcgcccat ggatcgcgca aatgagagga tcatgcccgc
     3001 gatgatgccc ggcaaga
//
LOCUS       DATMGX010000807         2189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_207927, whole genome shotgun sequence.
ACCESSION   DATMGX010000807 DATMGX010000000
VERSION     DATMGX010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207927"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VNR65_13245"
     CDS             <1..266
                     /locus_tag="VNR65_13245"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ19684.1"
                     /translation="QPDNARYSYVYAVALNSAGHADGAIAVLQQAHQRRPADRQVLMG
                     LISFERDKGNLPSAITYAQQLVQLVPDDPNAKAILAQLVAQRR"
     gene            complement(571..>2189)
                     /locus_tag="VNR65_13250"
     CDS             complement(571..>2189)
                     /locus_tag="VNR65_13250"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWJ19685.1"
                     /translation="SQFEQSHSHKAEDSSKQIEGTVIAVSAESVFLDIGFKSEGILPL
                     AAFQSAGEEVKPGDKLVVSVKGRDTDGYYELSRFKTALPKNWSSLEQAFADKTTIMGT
                     VTGVIKGGLSVDVGVRAFMPGSRSGARDAAEMEKLVGQEIRCRIIKLDVTDEDVVVDR
                     RAVTEEEERSGKERRYSEVKEGETVHGTVRSLTDYGAFVDIGGVDGLLHVGDIAWSRV
                     AKPADVLSVGQEIEARVLKIDPEKRRISLGMKQIQPDPWHSAAEKYTAGERVRGTVTR
                     VVDFGAFVELEPGVEGLIHISEMSWAKKARQASDVVKPGETVEAVVLAVSPSDRRISL
                     GLKQALGDPWAEVTQKFGVGSVIEGPVTSLTNFGAFVQLSEGVEGMIHVSEISAEKRI
                     NHPQEVLKVGQLVKTQVLAIDKERRLLRLGMKQLVPTSLDEYIAEHKEGDVVTGRLIE
                     VSDGLARVELGEGIQGTCPIVAERPGKEETRAESKADLSALSSMLKARWKGGAAGGAS
                     KPEAIRAGQIRSFRIAKLDAVAKKIELELA"
BASE COUNT          399 a          731 c          586 g          473 t
ORIGIN      
        1 tgcagccgga taacgcccgc tatagctatg tttacgccgt ggcgcttaat tccgccggac
       61 acgccgacgg cgcgattgcc gtgttgcagc aagcccacca aagacgtcca gccgaccgcc
      121 aggtcctgat ggggttgatc tccttcgagc gcgacaaagg aaacttgccg tcggcaatta
      181 cctacgcgca gcagctagtt caacttgtcc ccgatgaccc caatgcgaag gcgattttgg
      241 cgcagttggt cgcacagcga cgttgaatcg gcgagccccg ctcacaacag cagcttcacg
      301 gcagcgggga tgacagtgtc gaatggaatg gaagcgatca tgaccggaat tgccgggatt
      361 gtccttagaa ggaatgtgct gccaagtcaa agagtgggcg aattgcactc cgcagtgata
      421 gtggacgctg tcttcggtat tgcagcccga tgtggccttg cacagccttc aggtcgtcga
      481 cctcttgcga gcgaacggat tgcgatatcg cacgcgctga gcccgcactg cgactcctgc
      541 attcaaaggc ctcattgcgg cattagctta ttacgctaat tccaattcaa tcttcttcgc
      601 gaccgcatcc agcttcgcaa tccgaaaact gcgaatctgt ccggcacgaa tggcttccgg
      661 cttggacgcg ccgccggcag ccccgccctt ccagcgcgcc ttcagcatgg aactcagcgc
      721 tgacaggtcc gccttcgatt cggcccgcgt ctcttccttc cctggacgct ccgcgacgat
      781 tgggcacgtg ccctggatgc cttcgcccag ttccacgcgc gcgagcccgt ctgaaacctc
      841 gatcaggcgt ccggtgacca catcgccttc tttgtgctct gcgatgtatt catccaggct
      901 ggtgggaacc agttgcttca tccccagccg caggagccgc ctttccttat cgatcgcgag
      961 cacctgggtc ttaaccagtt gcccaacctt cagcacttct tgcggatggt tgatgcgctt
     1021 ctcggcgctg atttcgctga cgtgaatcat cccttcgacg ccttcggata gctgcacgaa
     1081 cgcgccgaag ttggtaaggc tggtgaccgg tccttcaatc accgagccaa ccccaaactt
     1141 ctgtgtcacc tcggcccagg gatctcccag ggcctgcttt agtcccagcg agatgcggcg
     1201 gtcactcggg ctgactgcca gaaccaccgc ttcaacggtc tcgcccggct tgaccacgtc
     1261 gctcgcctgc ctcgccttct tggcccacga catctcggaa atgtggatca agccttcaac
     1321 cccgggctca agttctacaa aggctccgaa gtcgacgacg cgcgtcacag tgccccgcac
     1381 gcgctcgcct gccgtatatt tctcggccgc cgaatgccag ggatcgggct gaatctgttt
     1441 catgcccagc gaaatgcgcc gcttctcagg atcgatcttg agaactctgg cttcgatctc
     1501 ctgccccacc gacagcacgt cagcaggttt cgcaacgcgg ctccaagcga tgtcgccgac
     1561 gtgcagcagt ccgtccactc cgcctatatc gacgaaagcg ccgtaatcgg tgagactgcg
     1621 gaccgtgcca tgcaccgtct cgccttcctt gacctcggaa taccggcgct ccttcccgct
     1681 gcgttcttcc tcttccgtca ccgcacggcg gtccaccacc acgtcttcgt cggtcacgtc
     1741 gagcttgata atccggcagc ggatctcctg ccccaccaac ttctccattt cggcggcgtc
     1801 gcgtgctcca ctgcgcgagc ctggcatgaa cgctcgtact cccacatcca cgctcaagcc
     1861 gcctttgatc acgcccgtca ccgtgcccat gatcgtcgtc ttgtcggcga aggcctgctc
     1921 cagcgatgac cagttcttgg gcagggcggt cttgaagcgg gaaagctcgt aatagccgtc
     1981 ggtgtcgcgg cccttcaccg aaacgaccag tttgtcgccg ggtttcactt cttcgccggc
     2041 gctctggaac gctgccagcg gcaggatgcc ttcgctcttg aaaccgatat cgaggaaaac
     2101 cgactcggcg gagacagcaa tcaccgtgcc ttcgatttgt ttactgctgt cttcggcctt
     2161 gtgcgaatgg ctctgctcga actgggaaa
//
LOCUS       DATMGX010000808         2157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_63470, whole genome shotgun sequence.
ACCESSION   DATMGX010000808 DATMGX010000000
VERSION     DATMGX010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63470"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VNR65_13255"
     CDS             <1..291
                     /locus_tag="VNR65_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19686.1"
                     /translation="RDSPIRAGLLQQKEGAARVECPILFGFGKFGLAPSNSSAAWTLQ
                     EYIAKSPLAHDVEPVHHELNRPVGLPFEDWPGGVQQFFAKPRALRWLSDALC"
     gene            663..1088
                     /gene="arfB"
                     /locus_tag="VNR65_13260"
     CDS             663..1088
                     /gene="arfB"
                     /locus_tag="VNR65_13260"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HWJ19687.1"
                     /translation="MIEITDRIALNEAEIAVTFIRASGPGGQNVNKVSSAAQLRFDVR
                     RSPSLPDAVRLRLERLAGTRLTKDGEIVFTAGNYRTQEANRRDAIDRLVRMIRQAAIE
                     RPVRRPTRPTLGSKQRRLTGKTKRGEVKRQRRAPGAIGD"
     gene            complement(1358..>2157)
                     /locus_tag="VNR65_13265"
     CDS             complement(1358..>2157)
                     /locus_tag="VNR65_13265"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HWJ19688.1"
                     /translation="TRLDLKLYPVPKSILASTYIFPLSEVEKVMNNLDAIRKANRICD
                     NVEPIAVLMHHPEVPADAPPEKSKIVFFTAFAFEDTEENARASLKPFEQLPEGAKLAI
                     KMEYQPFDFEGLYARYFSLNDPPGRCARYAVDNILTNEGGKALVALADHFKKAPAKDS
                     HILTALNLRLEEKKDSCFSWSADTFVACYSIWDDEKDDPANYAWLLENLPIMDPFAVG
                     HYVNEIEGRGHPERYPQCFSAANWERLQQLRKQYDPAGVFHSYLGHS"
BASE COUNT          440 a          609 c          673 g          435 t
ORIGIN      
        1 cgcgacagtc caattcgagc cggattgctt cagcagaaag aaggagccgc cagggtcgaa
       61 tgccccattc ttttcggctt cggcaaattt ggtcttgctc caagcaattc ttctgccgcc
      121 tggacgctgc aggaatacat cgccaaaagc ccactcgccc atgacgttga gccggtgcac
      181 cacgaactca accggcccgt cggtctcccg ttcgaagact ggcctggcgg cgtccaacag
      241 ttctttgcga agccgagagc cctgaggtgg ctgagtgacg ctctgtgctg acgcacatcc
      301 aaatcctaga atcaaaagta caagcgatag aacgagtttg cgcatggaag tcattttccg
      361 cttaatgaaa ggatcggccg aggattacga gatcaacccg ccttttaatc ggaatcgatt
      421 gagggcgcaa agttgatcga ttctaagagc tgcgtcctgc gcggtcccca tccgagcccc
      481 ctgaatcgag ggtgttagca agcggtttcc agatcaaatg ttcctcgcag agattggcca
      541 gccttaaaca tcgacggcac tcagtttgca tcaaaagtgc tagaagagaa gaggcgcgct
      601 ttgaggtttc ctctagcgaa cagcgttgaa agcgaatggc ggacgagtgg gcaggatcac
      661 ctatgatcga aattaccgac aggatcgccc ttaacgaggc cgaaatcgcc gtgaccttta
      721 tcagggcgag cggccctggc gggcagaacg tgaacaaagt atcgagcgcg gctcaattgc
      781 gcttcgatgt gcgccgctcg ccaagcctgc cggacgcggt gcgcctgcgc cttgagcgcc
      841 tcgcgggcac gcggctgacg aaggatggcg agatcgtttt cacggccgga aactaccgca
      901 cgcaggaggc caatcgcagg gatgccatag accggcttgt ccgcatgatc aggcaggcgg
      961 ccatcgagcg ccccgttcgc aggcccacgc ggccaacgct tggctcgaag cagcggcggc
     1021 tgaccggtaa gaccaagcgc ggcgaggtga agcgccagcg gcgcgcgccg ggtgccatcg
     1081 gcgattaggg cctcatgggg agtgatgtct cctgaacctg atggcgttcc taaaattaaa
     1141 ttgcttatat tcacaggatg atgcgcctgg aaaagcttag aatatatgag gcgttgccgc
     1201 tgccagcctc attgcgccgg cagtttggct ctcttttcgg gcgaaccgct ccttaccgga
     1261 tggcgattgt cgctgaaccg acagcggctg agtgcgatct gcaaggcggc gttagccgcc
     1321 attgcgatcc acaagcggaa gaggttctct ctccgcctta gctgtgcccc aggtagctgt
     1381 ggaacacgcc cgctgggtca tattgcttgc gcaactgctg caggcgctcc caattcgccg
     1441 cgctgaagca ttgcggatag cgctctggat ggccgcgccc ttcgatttcg ttcacatagt
     1501 gaccgacggc gaaggggtcc atgatcggca ggttttcgag cagccacgcg tagttggccg
     1561 ggtcgtcctt ctcgtcgtcc cagatggaat agcaggcgac gaaggtatct gccgaccagg
     1621 agaagcagga gtccttcttt tcctcgagcc gcagattgag cgcggtcagg atgtgggaat
     1681 ccttggcggg cgccttcttg aaatggtcgg cgagcgcaac aagcgccttg ccgccctcgt
     1741 tggtcaggat gttgtccacg gcgtagcgcg cgcagcggcc gggcggatcg ttcaggctga
     1801 aatagcgcgc atagaggccc tcgaagtcga agggctggta ctccatcttg atcgccagtt
     1861 tggcgccttc cggcagctgc tcgaagggct tcagggaggc gcgcgcattc tcttcggtgt
     1921 cctcgaaggc gaacgcggtg aagaagacga tcttcgactt ctccggcggc gcgtcggcgg
     1981 gaacctcggg atggtgcatg agcaccgcga tgggctcgac gttgtcgcag atgcggttcg
     2041 ccttgcggat cgcatcgaga ttgttcatca ccttctccac ctcggacagc gggaagatat
     2101 aggtgctggc caggatggat tttgggaccg gatagagctt gagatcgagg cgcgtca
//
LOCUS       DATMGX010000809         2169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_124067, whole genome shotgun sequence.
ACCESSION   DATMGX010000809 DATMGX010000000
VERSION     DATMGX010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124067"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VNR65_13270"
     CDS             complement(<1..366)
                     /locus_tag="VNR65_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWJ19689.1"
                     /translation="MKLSRYFLPVLRDDPKEAEIVSHRLMLRAGMIRQASAGIYSWLP
                     LGFRVLKKIEQIVREEQNRAGAIELLMPTIQSAELWQESGRYGDYGAEMLRIKDRHER
                     DMLFGPTNEEQITQIFRDAV"
     gene            895..1215
                     /locus_tag="VNR65_13275"
     CDS             895..1215
                     /locus_tag="VNR65_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19690.1"
                     /translation="MGRFSQNASHAFARPKSRGHRSAHLANVVEYGLLSMLIIIVVSI
                     GATLAGNKLNGVFGTVAMAMEQPGRSQNVLPSRPTSFGQTTNRKGAPVERDVSGKLSA
                     LSPF"
     gene            complement(1627..>2169)
                     /locus_tag="VNR65_13280"
     CDS             complement(1627..>2169)
                     /locus_tag="VNR65_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HWJ19691.1"
                     /translation="GLVLLLVLYLFAGLLFSMLRSLTAIGAGAGRLAQGDLSKRVDSH
                     STDELHDVGGAVNHVAETLQKFTKAQLDMARAHNHYGSISATIDEAQFSGAYRDIARN
                     LNEMVQAHIDVQTQFADLMVEYVDGKFNSRMAKLPGEQQKISDAAEKVRAGLAAAAEA
                     CAKCTRTAALACQHPAEHRQ"
BASE COUNT          443 a          669 c          617 g          440 t
ORIGIN      
        1 caccgcatcg cggaagatct gcgtgatctg ctcctcgttc gtcggcccga acagcatgtc
       61 gcgctcatgg cggtccttga tgcgcagcat ctccgcgcca taatcgccgt agcgcccgct
      121 ctcctgccag agctccgcag actggatggt gggcatcaag agctcgatgg cgccagcccg
      181 gttctgctcc tcgcgaacga tctgctcgat ctttttcaga acgcgaaatc caagcggcag
      241 ccacgaataa atcccggcgc tagcctggcg gatcatgccc gcccgcagca tgagccgatg
      301 ggagacgatt tcagcctctt tgggatcgtc gcgcaaaacc gggaggaaat aacggctgag
      361 tttcatgagt ctgctccgcg ggtcgcctgc ggtggcggcc taagccgagg gacatagacc
      421 tttccaaaag cgagtgcaat gcggggggca attttatcaa aaagatggaa acgattttca
      481 gacacattca gaatctgtga attggcaccc tcggaaccgg actcagcctc aaggccgcat
      541 aaaacgacga gagagggcag cgcctatctg gccagttaga tacttacgta gcccgcccat
      601 gaccattgcc gaactcccgc ctcgccagtc cagccggcat tacctcgtcc gcttctggga
      661 aatgctgaca accgcgacga gggagagccg gaaagcccaa gggcttgcca gccgttccct
      721 tgcttcgaac catgagcgcg ccccaaggct gatgcgctgg gccgtaacaa gcgtattcgt
      781 gaggacggtt aaatcatcat tttaaatcag gcgcaaaaac agagccgcct cttggcgaat
      841 aagaaattcg cgggtggtaa gaataatata gcaagagagc caagtgagtg tgccatgggt
      901 cgcttcagcc aaaacgcaag tcatgccttc gctcggccca agtctcgagg ccaccgatca
      961 gctcatctgg ccaatgtggt cgaatatggc ctcctgtcga tgctgatcat catcgtggtt
     1021 tcgattggcg cgacactggc gggtaataaa ctcaacggcg tctttggcac cgtggccatg
     1081 gcaatggagc aacccggcag gtctcagaac gttctccctt cgcggccgac atcgttcggc
     1141 caaacgacga atcgcaaagg cgcgcctgtc gagcgcgacg tgagcggcaa gctttccgct
     1201 ctcagtccct tttaactttt ccgggaacct tctcacacgc cgaagggacc atacgcgcct
     1261 cagggctgat gcgctggcgt ggcaagcttt gcctgcgccc cctcgcccta atgtttcctc
     1321 cgtggccaag ctaacaccct attcgcgggc tctcttgccg acgcacggcc caaggtcaaa
     1381 tcccttaagc ggcccagcgc ctcagctgcc gctttcgcca tcatgagcga aagcgcgggc
     1441 ggcattggtt gcctgtgatc tcttcgaggc ttgacgcctg cgttcagtgc gctccgagag
     1501 atcgttgttg ccgatcacgg ctttgcacaa cgccgcacgg cgccaagggt cgcgttcagc
     1561 tcctgcgccg ccctccacga aagccgccac ctcctggcgc ggctcatagt ggggatttgt
     1621 ccctcgtcat tgacgatgct cagcggggtg ctgacatgcg agcgctgccg ttcgcgtgca
     1681 tttggcgcag gcttcagccg cagctgccag cccggcgcga accttttcag cggcgtcgga
     1741 aatcttttgc tgctcgccag gaagctttgc catgcggctg ttgaatttgc catcgacgta
     1801 ctcaaccatc agatccgcga actgcgtttg cacatcgatg tgagcttgca ccatctcgtt
     1861 gagattgcgc gcaatgtcgc ggtaggctcc agagaattga gcttcatcga tcgtcgcgct
     1921 gattgagcca tagtgattat gcgcgcgggc catgtcgagc tgagccttgg tgaacttttg
     1981 gagggtctcc gccacgtggt tgaccgcgcc gcccacgtca tgcaactcgt ccgttgaatg
     2041 gctgtcgaca cgctttgaga ggtcgccttg cgccaaccgc ccggcaccgg ctccgatggc
     2101 cgtcagcgag cgcagcatcg aaaagagcag gcctgcgaag agatacagca ccaggagaag
     2161 caccaaacc
//
LOCUS       DATMGX010000810         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_169644, whole genome shotgun sequence.
ACCESSION   DATMGX010000810 DATMGX010000000
VERSION     DATMGX010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169644"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            571..1485
                     /locus_tag="VNR65_13285"
     CDS             571..1485
                     /locus_tag="VNR65_13285"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HWJ19692.1"
                     /translation="MKFGTATINETQHQFIKDYKEALEKASGGRIEVQVYPASQLGPI
                     PREIEGVQLGTIQGYIGPVDFYVGLEPRYGVFSAPMLFRDEAHVAATIHDPEMQKIIL
                     DLAKPKRMVGIATLSIGVVEYGAKSPLMRLADFDGKKIRINGTALERAKMSKLGATGI
                     AMPLSEVMPALSQGTIDGTISGMSVFVGFKMNNLVKVISVTNDTMLVSLAVVSQAWLD
                     KLPPDLQKIVLDTAQAVQPKVQAWEVEFTKKLADDWVKMGGQVHTLPPEDLAKMKTLL
                     SGVADDVTKDQPAVHDMLVKVRAIAAKH"
     gene            1573..2085
                     /locus_tag="VNR65_13290"
     CDS             1573..2085
                     /locus_tag="VNR65_13290"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HWJ19693.1"
                     /translation="MINTLVRSAHALMAVIRVASGALLVGSVAINFANIIGRYFLSVS
                     IPWAEEVMLFMMIACVFLNCGLVGWSGRQIRMDAFVALLPPKLRSIVELLGEIVLMAT
                     SLLIAILAWPVVSMLAELDQRSQAANIPLVLPQAMIPVGFLLMALLIAVRLISQGTSG
                     ADAHLKNSDH"
BASE COUNT          467 a          623 c          641 g          471 t
ORIGIN      
        1 ggggacgagt attgcttcct gcccccagca caaataaatt gtttcgaatg ctgcaaagca
       61 cactcaatgt ccgctctggg tcattagcgg acaaaccatc acgagctaaa atccagtttt
      121 gtccgctttg gtccaaaagc ggacaaacgc gggtacgctt ggattgtccg ttttgtgcaa
      181 gagcggacgt tggcgtacta tgactgtatg caacgtctag ggatcatccg ccttgggccg
      241 taattgatgt aaacctcgaa tgatggatct attttgtcga aacgcggcac gaattagtgg
      301 cgcccaccgc cagcttggct gatacaaaca tcaaggcatc aatgatagct tgaggaggac
      361 gcgcaagatc cctaacatca cccgcaatcg acaccgtccg tcgggtcaaa ggcccaacca
      421 agggccccta cgaggcggtg acaaaagtaa ctaggggagg atgtgatgat cagagtacgc
      481 agcatcacgc cgtgggcgtg gacgattgga ctactggctg cacttgcgtg cgctgccccg
      541 agcagcccgg ccgcgtccgc agaatacgta atgaaattcg gcaccgcgac catcaacgaa
      601 acgcagcatc agttcataaa agactacaag gaagcgctcg aaaaggcgtc gggtggccgc
      661 atcgaggtgc aggtctatcc cgccagccag ctcgggccga tcccgcgcga gatcgaaggc
      721 gtgcagctcg gcacaatcca gggctatatc gggccggtcg atttctacgt cggtctggag
      781 ccgcgctacg gcgtattcag cgcgccaatg ttgttccgcg acgaggcgca tgtcgcggcg
      841 accattcacg atcccgagat gcaaaaaatc attctcgacc tcgcaaagcc aaagcggatg
      901 gtcggcatcg ctacgctcag catcggggtc gtagagtatg gggccaagag tccgctgatg
      961 cggcttgctg acttcgacgg caagaagatt cgaatcaatg ggaccgcgct cgagcgcgcc
     1021 aagatgagca agctcggtgc caccggcatc gcgatgccac tgagcgaagt gatgcccgca
     1081 ctcagtcagg gcaccatcga cggcacgatc agcggaatgt cggtctttgt cggattcaag
     1141 atgaacaatc tggtcaaagt catttcggtt accaatgata caatgctggt gtcgctggct
     1201 gtcgtcagtc aggcatggct cgataaattg ccacccgatc tgcagaagat cgtcctcgat
     1261 actgcgcaag ccgtccagcc caaggtgcaa gcctgggagg tggaattcac aaagaagctg
     1321 gcggacgatt gggtgaaaat gggcgggcag gttcacacgt tgccgccgga ggacctcgcc
     1381 aagatgaaga cgctgcttag cggcgtcgcc gacgatgtga cgaaagacca gccggctgtg
     1441 cacgacatgc tggttaaggt ccgcgccatc gccgcgaaac attgatccgc tcgggcagct
     1501 acggcggttg atctggccgc cccagcctcg cgcccgatcg cccgaagggc gtattccgta
     1561 aataggtgag ggatgatcaa cactcttgtg cggagcgcgc atgcgctgat ggcagtcatt
     1621 cgcgtcgctt cgggagcgct gctggtcggc agcgtcgcga tcaacttcgc caacatcatc
     1681 ggtcgttatt ttctctccgt gtccatcccc tgggccgagg aggtcatgct tttcatgatg
     1741 atcgcctgtg tctttctcaa ttgcggcctg gtcggctggt cgggccggca gatccgcatg
     1801 gatgcatttg ttgctctgct gccgcctaag ctgcggtcga tcgtcgagct tttgggcgaa
     1861 atcgtgctga tggcaaccag cctgctgatc gcgatcctgg cctggcccgt ggtttccatg
     1921 ttggcggagc tagatcagcg gagccaagcc gccaatattc ccctagtcct gccgcaggcc
     1981 atgattccag tcggcttcct gttgatggcg ttgctgatcg ccgtgcgatt gatttcccaa
     2041 ggcacgtccg gcgcggacgc gcatttgaaa aattctgatc actgaacgat tcagcgcatg
     2101 cgggcccaat tcgtctactt cgttctgccg atcattctta tggtggtcgg ccttccgatt
     2161 ttccttgttc tcatcctgac ctcactcgcc gcgattgtct tt
//
LOCUS       DATMGX010000811         2567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_453672, whole genome shotgun sequence.
ACCESSION   DATMGX010000811 DATMGX010000000
VERSION     DATMGX010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453672"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..497)
                     /locus_tag="VNR65_13295"
     CDS             complement(162..497)
                     /locus_tag="VNR65_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19694.1"
                     /translation="MQAPLQISFEGVLRSDAIEGDIREEWETLNDLGAPIISSVVVVT
                     QPRAKHFSGDPCRLRVHLTVEGGPDINVSHDPGPGRRHDAMRVAVRDTFRIVRRRLED
                     SMRRREPLS"
     gene            complement(598..924)
                     /locus_tag="VNR65_13300"
     CDS             complement(598..924)
                     /locus_tag="VNR65_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19695.1"
                     /translation="MERKRDHVQLISEDILKELLNVEGRYIEWEWDPRTLTNDYITMR
                     EPDGLQVSIRNMGGRSRYLNVTLRALEELIQEHLVSEDPTRKVTGFRLYRLNAEGRKR
                     GHRVLA"
     gene            1326..1559
                     /locus_tag="VNR65_13305"
     CDS             1326..1559
                     /locus_tag="VNR65_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19696.1"
                     /translation="MATSRSKAEDRYAQLRKREQGLTQEVDAAARQRAEKTAKLRQLR
                     LAKEAEDAAEKQAAKDIKAAGKSAFKKPRSSAP"
     gene            complement(1815..2273)
                     /locus_tag="VNR65_13310"
     CDS             complement(1815..2273)
                     /locus_tag="VNR65_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HWJ19697.1"
                     /translation="MTLQGVKPAFLAIIATVAAVVAPAPAFAQNAAGAQKQRQAPTPT
                     PPKPAATPDDRPYDAQLFRLAEILGTLHYLRELCGANEGQVWREHMRELTASEGTSAL
                     RRAKLVESFNRGYRDYSRTYRTCTQPAIVAIQRYIEQASAITDGLIANDK"
     gene            complement(2270..>2567)
                     /locus_tag="VNR65_13315"
     CDS             complement(2270..>2567)
                     /locus_tag="VNR65_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWJ19698.1"
                     /translation="TAADAARRELQEETGLLALNLTLGDVADGILRDGIGTVTAHYTI
                     IVFATSDVQGILMPASDAAAAGFFGPEERTRLQRTPGLENAIQNARCALERDSR"
BASE COUNT          577 a          699 c          705 g          586 t
ORIGIN      
        1 cataacgcaa acctttctgt atgttcagtt tcgtgaaact taagaagtag aaacttttat
       61 atcaggaaga aagcgtttcg attttctcat ctgtcgcaaa tagtctggtt tatatttgca
      121 agtgacgacc ctcaatcggt ccgcacgatc tgtcgccgtc gctatgaaag gggttctctt
      181 ctccgcatgg aatcctccaa gcgccgccgc acaatgcgga aggtgtcgcg gactgctaca
      241 cgcatggcgt catggcgtct tcccgggccg ggatcgtgac tgacgttgat atcggggccg
      301 ccttcgaccg taagatggac ccggagacga cacgggtctc cggaaaaatg cttcgcgcgc
      361 ggttgggtaa cgacaacaac cgaagaaatt atgggcgcgc cgagatcatt gagagtctcc
      421 cactcctcgc gaatgtcccc ttcgattgca tcggagcgca ggacgccttc aaagctaatc
      481 tggagcggag cttgcatgca ggagcgcctt ttgttggcag ccgtccgaga tgacggcgcg
      541 ggattgaaaa atcgaattgg cgagagaatt gcgatctgcg tgatcgcctt aatccactta
      601 cgctaacacc cgatggccgc gtttccgccc ctcggcatta aggcgataaa gccggaaacc
      661 agtgactttt ctcgttggat cttcggaaac aagatgttcc tggatcagct cctccagagc
      721 cctgagcgtc acgttgaggt aacggctgcg tccgcccata tttctaattg atacctgcaa
      781 gccgtcgggt tcgcgcatgg taatgtagtc gtttgtcaat gtgcggggat cccactccca
      841 ctcgatgtag cggccttcga cgttcaggag ctccttgagg atgtcctcgg aaatgagctg
      901 cacatgatct cgctttcttt ccacggtttt agcgcatatg gggtggccgc gccaaattaa
      961 aacgagactg gctggcaccc agggctctaa attttataac gattgcattc gtctaatctc
     1021 acccatgcca agcgatgtgc aaaacgaaga aggccagatt acgaagaatt tcaacttttt
     1081 taacgagaaa atacgaaagt ttcctacctg taaaacacct gaagacttgc aataaacatt
     1141 ccgtcaacga tatataaaat cgagccacgg cggggacgtc aatctttctt cgatatggta
     1201 tggtcggtca gtcgttgtgt tctgcggcat attttgcaga cggcccagct ggaatttggc
     1261 tgacaagtcg ccccaaccat ctttacaaaa ttttcggcat gcgccgcacg aagggactct
     1321 agataatggc cacatcccgg tcgaaagctg aggatcgcta cgcccaactt cggaagcgcg
     1381 agcagggcct gactcaggag gtcgatgccg cagcacgcca gcgggctgaa aaaacagcca
     1441 aactgcgcca attgcggctg gcaaaagaag ctgaagatgc tgctgagaag caggccgcga
     1501 aagacatcaa ggccgccggc aaaagcgctt tcaagaagcc gcgttcaagc gcaccatgat
     1561 ccttcaaggg cgaggtattt actgctagct ttcggaagtc gagcagacgc ttttcaactt
     1621 tcctgccatg atctgatctg agcctgccaa tctgggtcca gcacaccaag gatggtccgg
     1681 ctgggagcga gcttaatacc gactttcagt tcggtcacag ccccattccg tgggaccagg
     1741 aaaactagtt atatcttacg aaacaaaccc aacttggtta accgacagtg tcccctcata
     1801 ggaggcggtt ggtattattt atcgttggcg atcagcccgt cggtgatggc cgatgcctgc
     1861 tcgatgtagc gttggatcgc gacgatcgca ggctgagtgc atgtgcgata ggttcgcgaa
     1921 tagtcgcgat agccgcgatt gaagctttcc acaagcttcg cacggcgcag cgcgcttgta
     1981 ccctcgctcg ccgtaagctc gcgcatgtgt tcgcgccaga cctgtccctc attggctcca
     2041 catagctcgc gcaggtaatg caaggtgccg agaatttcgg cgagtcggaa aagctgtgca
     2101 tcgtaagggc ggtcatcggg cgtcgctgca ggctttggcg gggtaggcgt aggcgcctgg
     2161 cgttgtttct gcgctcctgc tgcgttctgg gcgaaagcag gggcaggcgc tactactgca
     2221 gcaacagtcg cgatgatggc cagaaaggcc ggttttaccc cttgcaatgt catcggctgt
     2281 ctctttccaa ggcgcatctc gcgttctgga tcgcgttctc caaccctggc gttcgttgca
     2341 gtcgcgttct ctcttcgggt ccaaagaagc ctgccgctgc cgcgtcggaa gccggcatga
     2401 ggatgccctg aacgtcgctc gtggcaaaga cgatgatcgt ataatgcgcg gtcacggtgc
     2461 cgatgccatc ccttagaatc ccatcggcaa catcccccaa agtgagattc aaggcaagca
     2521 gccccgtttc ttcctggagt tcgcgccgtg cggcgtcagc ggccgtc
//
LOCUS       DATMGX010000812         3214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_418521, whole genome shotgun sequence.
ACCESSION   DATMGX010000812 DATMGX010000000
VERSION     DATMGX010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418521"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VNR65_13320"
     CDS             complement(<1..1415)
                     /locus_tag="VNR65_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWJ19699.1"
                     /translation="MKFLRSEERNAKHSGPAWAGFTALIAIAALGCSMNAAASAEPDR
                     TVLPLVEPPRQVSTELDVRNATPPPRFEVKAPSGAPNVLLILIDDLGFAGTSAFGGPV
                     STPSFDKIAREGLVYNDFHTQATCSPTRAALLSGRNHHVANMGGIAESGTAFPGNTTQ
                     IPKEVAPLAEMLRLNGFSTAAFGKWHQTPPWETSISGPFDRWPTGQGFDKFYGFIGFS
                     TNHWSPAIIEGTSRIEPPQRPGYNFLTDMTNQAIDWLSFQKALTPDRPFFLYFAPGAV
                     RVPHHVPEEWAQRWKGKFNQGWDKMREQALARQIELGVVPKDTKLTPRSPAIPAWDSL
                     TDDERRLFTRQVEVFAGFLEYTDHEIGRLIEAVRKTGQLDNTLIIFVAGDNGSSAEGG
                     RNGTLNEFTYFNNVEPTVKELLQHLDDWGGPKTLPHMAAGWSQVFGTPFEYVKQVASD
                     YGGTKVGMAIRWPQGGVPGNG"
     gene            complement(1412..1624)
                     /locus_tag="VNR65_13325"
     CDS             complement(1412..1624)
                     /locus_tag="VNR65_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19700.1"
                     /translation="MLSKIVVMALLIASIGITAVFGSAAAQQAIMGSLYPFCERTPMA
                     QAASNNSRSPSGNVGLQAMELMGGGR"
     gene            2443..2603
                     /locus_tag="VNR65_13330"
                     /pseudo
     CDS             2443..2603
                     /locus_tag="VNR65_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011653555.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
BASE COUNT          681 a          926 c          901 g          706 t
ORIGIN      
        1 tcgccattac ctggcacacc tccctgtggc cagcgaattg ccatgccaac cttcgtgcca
       61 ccataatccg aggcgacctg cttcacatac tcaaatggtg ttccgaagac ctggctccaa
      121 ccggccgcca tatgaggaag agtcttcggt ccgccccagt catcgaggtg ttggagcagt
      181 tctttcacgg tcggctctac attgttgaag taggtgaatt cgttgagtgt gccgttgcgg
      241 ccgccttcgg cactgctccc attatcacca gccacgaaaa ttatcagcgt gttgtcgagt
      301 tgcccggtct tgcgcacggc ctcgatcaag cgaccgatct cgtggtcagt gtattccaga
      361 aatccggcaa acacctccac ctggcgggtg aacaggcgtc tttcgtcgtc tgtgagcgaa
      421 tcccaagcgg gaatggcggg cgaccggggc gtcagctttg tgtctttcgg aaccacgcca
      481 agctcgatct ggcgtgccag cgcctgctcg cgcatcttgt cccagccctg gttgaacttg
      541 cctttccatc gctgggccca ttcttccggt acgtgatggg gaacccgcac tgcgcctgga
      601 gcaaaataca agaagaatgg gcgatcgggc gtgagggctt tctgaaaact caaccagtcg
      661 atggcctgat tggtcatgtc cgtcaaaaag ttgtaacccg gacgctgcgg cggctcgatg
      721 cgcgacgtgc cttcaatgat cgcgggggac cagtggttgg tcgaaaagcc gataaagcca
      781 tagaacttgt cgaagccctg accggttggc cagcggtcga aaggcccaga aatactcgtc
      841 tcccacggtg gggtctggtg ccacttgccg aaggccgcgg tgctgaagcc attcaacctg
      901 agcatctccg caagcggcgc cacctccttg ggtatttgag tggtgttgcc agggaacgcg
      961 gtgccggact ccgctatgcc gcccatgttg gccacgtgat gattgcggcc gctcagcagt
     1021 gctgcacgtg tcggcgaaca ggtcgcctgg gtgtgaaaat cgttataaac cagaccctct
     1081 cgagcaatct tgtcaaagct gggtgtcgag accggtccgc cgaatgcgct cgtgcccgca
     1141 aaaccaagat catcgatcag gatcagcaga acgttgggcg ctcctgaagg cgctttgacc
     1201 tcgaagcgtg gcggcggagt ggcgttgcgg acgtcgagtt ccgtagacac ttgccgcggc
     1261 ggctcgacca gcggcaatac ggtgcgatcg ggttcggcgg acgccgccgc attcatcgag
     1321 cagccgagcg ccgcgatcgc aatcaatgcg gtgaacccag cccaagcagg accggaatgc
     1381 ttcgcattgc gttcctcgga ccgcaagaat ttcatcgtcc ccctcccatc agttccattg
     1441 cctgaagtcc gacattcccc gacggactgc ggctgttgtt ggatgcagcc tgcgccatcg
     1501 gagtcctctc gcagaatggg tacagagaac ccatgatcgc ctgctgtgcg gccgccgagc
     1561 caaaaacggc ggtaatgccg atgctggcga tcagaagcgc catgacgacg atcttgctca
     1621 gcacagagta atcttgatca tggcggcctc ttcgactgag ggattatccg cgcgaacgtg
     1681 ccgtccgcaa tccgggaatg gttgtggtgc tgagctaggg cgcgggagcg gacagcgcga
     1741 gtgcggttgt tttcaccaga ccgatcttcc cccagagata tttttattgc attatcgaga
     1801 ttaaagcata gcagctgaag cgatacaagg cagattcgat cttgccaata tagacaggtc
     1861 gagatcgcca gcttcggtga aaccggggat tgtgctcaag tatacgaaag ccagaatatt
     1921 tggcaataaa gaaacatgtt tttctaatac ctcaacaaga aggttcattc accttgccca
     1981 ataaaggcga ctcatcaacc agtgaatcag gagatcaacc tgggcggctg atgaggggaa
     2041 aatgcacggc gctcttcgac tttgaccgct attcgcatca acgctggctc ttttaacgct
     2101 tagtgccggt tcgacagcgg ccgctgacct ctcctcgccg cagccgcaag ccgttccgaa
     2161 cgccatctcc agcggctggg agtttcgttc acgccttacc tttggcgccg tggttctcgg
     2221 ggaaatgcca ccatcaaaga tcgtactttc gatgtccagg cgcacccttc cgatttcggt
     2281 gctttgaagt tcgcagtcat gggcgatctg ggggcacgca aaggcccttt cagtcttatg
     2341 gtggatggtt tttacggcaa ccaatgagac aaaggcgcgt gcgtattatc catcgaggcc
     2401 cttcgccacg gcccgcgagc cgatcaaaca ggccggatac atcgcaattc ccgcgccggc
     2461 atctaggcgg gcatatcctg tctgaagcgc gccttcgggc tcgcccgttg catgtggcgc
     2521 gggctcgagc gatgcaagga ttacgtctgg tcctcggagt ggcctataac ctcgccctgt
     2581 tcgcacgctt cgcggctgct tgaagcttcg gcggccgcga agctcaggcc aaccggcaat
     2641 agccggcgcc cccatttttc cggaagcatt taacactcgc tccgccgcct cccggtccgg
     2701 gagaaagcac gcatcccgta acaataagcg cctaaaaacg gaaacagcca tttatggatg
     2761 gacacttgct cgttagccac tctgtgaaag tcgccgtctc ggcacctcgc agccgctcaa
     2821 aattgtgggt tgggtaaacc gggtctcggc tcgcggatat cgtgaaataa gcgcgaatct
     2881 cgctcggatt tgttcgggaa aactggtgta actgattgat ttgtctgagc tccaaaaaca
     2941 gacattcggg agctaagcta ttgttctgct gaaagaaagc agggcatcat aatccgcgtg
     3001 tcgggggttc gagtccctcc tccgctacca ataatttcaa tgagttagcc tcattttcag
     3061 ccttttgcgg ttaattttcc gccaaggact tgggttaaca aaatcaatag tttacgggcg
     3121 ttgcaaaccg ttgtcggtca ttccatgcaa cacgcctgca acacgaaaag cgcaaatgtt
     3181 cctgccatgt tccatgcgca agccttcgtc gatc
//
LOCUS       DATMGX010000813         3145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_203464, whole genome shotgun sequence.
ACCESSION   DATMGX010000813 DATMGX010000000
VERSION     DATMGX010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203464"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VNR65_13335"
     CDS             <1..295
                     /locus_tag="VNR65_13335"
                     /inference="COORDINATES: protein motif:HMM:NF004366.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWJ19701.1"
                     /translation="MKSAYQVLRRPVITEKGLGVKETEATLVFEVSLKATKTEIKQAV
                     QSIFKVKVDSVRTSTFAGKERRRGKFTGYRPDWKKAYVRLKAGEKMPEYAENL"
     gene            345..1169
                     /gene="rplB"
                     /locus_tag="VNR65_13340"
     CDS             345..1169
                     /gene="rplB"
                     /locus_tag="VNR65_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWJ19702.1"
                     /translation="MAIKTYRPVTPSRRFTTSTVNDDLTTNRPHKPLTEPKQRTGGRR
                     NSGDLTIWHRGGGHKRKLRIIDFKRDKFGIPATVASIEYDPNRSARIALLNYADGEKR
                     YIVYPVGLKVGAKIVSGPEADILVGNALPLRNIPPGTTVHNVELKPGKGAQMARSAGA
                     QAQLVAKEGDYALLKLPSGETRKVLVDCMATVGQVGNIDHENISIGKAGRTRWLGIRP
                     TNRGVVMNPVDHPHGGGEGKTSGGRHPVTPWGKPTRGYKTRNNKRTDKFIVNRRQK"
     gene            1182..1481
                     /gene="rpsS"
                     /locus_tag="VNR65_13345"
     CDS             1182..1481
                     /gene="rpsS"
                     /locus_tag="VNR65_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWJ19703.1"
                     /translation="MARSTKKGAFIDSHLQAKIDGMNARNEKKVVRTWSRRSTVIPEM
                     VGHTLAVHNGKKFIPVYVTENMVGHKLGEFSMTRSFKGHSVRAATETAARPAPGR"
     gene            1492..1971
                     /gene="rplV"
                     /locus_tag="VNR65_13350"
     CDS             1492..1971
                     /gene="rplV"
                     /locus_tag="VNR65_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWJ19704.1"
                     /translation="MEFTAKLNYVRVSPQKVRLVLDLIKGRRVEEALDTLLYAKKGIA
                     PKLYKLLRSAVENANYASAEKGLDIDVDRLYVKRAVANDGPRMKRIRPAPMGRAYHYQ
                     RRISHVEIALEEKASGNGHAGLVQTVEGAPAKKPVRKIVKKAPVKKKPAAKKPAKKK"
     gene            2031..2699
                     /gene="rpsC"
                     /locus_tag="VNR65_13355"
     CDS             2031..2699
                     /gene="rpsC"
                     /locus_tag="VNR65_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWJ19705.1"
                     /translation="MGQKVHPYGFRLGVTKPWKSRWFVVRDYDKLLLEDVKLKAELKE
                     KLKSAGVSSIEIERPGNKLRIIIRTARPGIIIGRKGTEIDKLKQDIQKRTNRDVFVDI
                     QEVHKPELDAQLVAENISLQLEKRVGFRRAMRKSVDSALRFGCKGIKVRVSGRLNGNE
                     IARSEWYLQGRLPLHTLRADIDYGFAESHTTYGIIGVKCWVYKGEIFKEKRREPQGVQ
                     SGAF"
     gene            2791..>3145
                     /gene="rplP"
                     /locus_tag="VNR65_13360"
     CDS             2791..>3145
                     /gene="rplP"
                     /locus_tag="VNR65_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWJ19706.1"
                     /translation="MLMPKKVKYRKQQRGRMTGKAWRGGELSFGDYGLKVMEPGWITD
                     RQIEASRVAMTRFVKRGGKIWIRLFPDKPVTKKPAETRMGKGKGAPDHWVAVVRPGKI
                     LFEMEGVSVSDATEAM"
BASE COUNT          764 a          860 c         1005 g          516 t
ORIGIN      
        1 aatgaaatcg gcatatcagg tcctgcgccg cccggtgatt accgaaaagg gtttgggggt
       61 aaaggaaacc gaagcgaccc tggtcttcga agtttccctg aaagcaacta agaccgagat
      121 caagcaggcg gtgcaaagca ttttcaaggt gaaagtggat tcggtgcgca cctcgacttt
      181 tgcaggcaaa gagcgtcggc gcggcaagtt tacgggatat cgcccggact ggaagaaagc
      241 ttacgtgcgg ttgaaggcgg gcgagaagat gcccgagtac gccgaaaacc tctagttagc
      301 gcctagtggt tagaggctag aagtattgag aaacgagtag acacatggct atcaagactt
      361 acagaccggt tactccgtcg cggcggttca ccacgagcac ggtcaacgat gacctgacga
      421 cgaaccggcc gcacaagccg ctgaccgaac ccaagcagcg caccggcgga cgccgcaact
      481 ccggcgatct gaccatctgg caccgcggcg ggggacacaa gcgcaagctg cgcatcatcg
      541 acttcaagcg cgacaagttc ggaattcccg cgaccgtcgc ctccatcgag tacgatccca
      601 atcgctcggc gcgtatcgcc ttgctgaact acgcggatgg cgagaagcgg tacatcgtgt
      661 acccggtcgg cctgaaggtt ggcgccaaga ttgtcagcgg ccccgaggcc gacatcctgg
      721 tgggcaatgc gctgcccttg cgcaacattc ctcccggcac cacggtccac aacgtcgagt
      781 tgaagcccgg caagggagca cagatggcgc ggtcggcggg ggcgcaggcg cagttggtcg
      841 cgaaggaagg cgactacgcg ttgctcaaac tgccttccgg cgaaacccgc aaggtgctgg
      901 tcgactgcat ggccaccgtt gggcaggttg gcaatatcga tcacgaaaac atttccatcg
      961 gcaaggccgg ccgcacgcgc tggctgggaa tccgcccgac gaaccgcggc gtggtcatga
     1021 acccagtcga tcacccgcac ggcggcggcg aaggcaagac cagcggcgga cgtcacccgg
     1081 tgactccgtg gggcaagccg acgcgaggct acaagactcg caacaacaag cggaccgata
     1141 agttcatcgt gaaccgcaga cagaagtagg actgagacga tatggcacgt tcaacaaaaa
     1201 aaggcgcgtt catcgattcg cacctgcagg ccaagattga cggcatgaac gcacgcaacg
     1261 agaagaaggt tgtgcgcacc tggtcgcgcc gctccaccgt gatccccgag atggtgggac
     1321 acaccctggc ggtgcacaac ggcaagaaat tcattccggt gtatgtgacc gagaacatgg
     1381 ttggtcacaa actgggcgag ttcagcatga cgcgcagctt caagggccat tccgtgaggg
     1441 ctgcgaccga gaccgcagcc aggccggctc cggggagata aggaaaacga catggaattc
     1501 acagccaaac tgaattacgt gcgagtctct ccgcagaaag tgcgactggt attggacctg
     1561 atcaagggcc gccgggtgga agaggcgctg gacacgctcc tgtatgccaa gaagggcatc
     1621 gctccgaagc tgtataagct gctgcgttcg gcggtcgaaa acgccaacta cgccagcgcc
     1681 gaaaagggcc tggatatcga tgtcgaccgg ctgtacgtga agcgcgccgt ggctaacgat
     1741 gggccgcgca tgaagcgtat ccgtccggcg ccgatgggcc gagcctacca ctatcagcgg
     1801 cgcatctcgc atgtggagat tgccctggag gaaaaggcgt caggcaatgg ccatgccggt
     1861 ctggtgcaga cggtggaagg cgcaccggca aagaagccag tcagaaaaat tgtgaagaaa
     1921 gcgcccgtca agaagaagcc tgcggctaag aagccggcca agaagaagta gtagagattt
     1981 ataaaagcgg cgatgccaga gtgtggccgt cgcggagaag aaacgagact atgggacaga
     2041 aagtccatcc atacggattc cgcctcggcg ttaccaagcc gtggaagtcg cgctggttcg
     2101 tggtgcgcga ctacgacaaa ctgctgctgg aagacgtgaa gctgaaagcc gagctgaagg
     2161 aaaagctgaa gtcggctggc gttagctcca tcgagatcga gcgtcctggc aacaagctgc
     2221 gcatcatcat ccgcaccgcg cgtccgggga tcatcatcgg ccgcaaggga acggagatcg
     2281 acaagctgaa gcaggacatc cagaagcgca ccaatcgcga tgtctttgtc gacattcagg
     2341 aagtgcacaa gcccgagctc gatgcacaac tggtggcgga gaacatttcg ctgcagctgg
     2401 agaagcgcgt gggattccgg cgtgccatgc gcaagtccgt ggattcggca ctgcgcttcg
     2461 gctgcaaggg aatcaaggtg cgggttagcg gccgcctgaa tggcaacgaa atcgcccgct
     2521 ccgagtggta cctgcagggc cgcttgccgc tgcacactct gcgcgccgat atcgattacg
     2581 gttttgccga gtcccacacc acctacggca tcatcggcgt gaagtgctgg gtctacaagg
     2641 gcgagatttt caaggagaaa cgccgcgaac cgcagggtgt acagagcgga gcgttttaaa
     2701 gcagttgtca gtggccagtt gccagttgtc agaaagaggg cagcgaacca tggttactga
     2761 gaactgacaa ctgattactg ggaactgatt atgttgatgc caaagaaagt gaagtatcgc
     2821 aagcagcagc gcggccgcat gaccggcaag gcttggcgcg gcggcgaact gtcgttcggc
     2881 gattacggcc tgaaggtcat ggaaccggga tggatcaccg atcggcagat cgaagccagc
     2941 cgcgtggcca tgacccgctt tgtcaaacgc ggcgggaaaa tttggatccg gctgttcccg
     3001 gacaagcccg tcaccaagaa gccggctgaa acccgtatgg gcaaaggcaa gggagctccg
     3061 gatcattggg tggccgtagt gcgtccgggc aagatcctgt ttgaaatgga aggggtctcg
     3121 gtgagcgatg ccactgaggc gatgc
//
LOCUS       DATMGX010000814         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_234976, whole genome shotgun sequence.
ACCESSION   DATMGX010000814 DATMGX010000000
VERSION     DATMGX010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234976"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VNR65_13365"
     CDS             complement(<1..752)
                     /locus_tag="VNR65_13365"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HWJ19707.1"
                     /translation="MKKNQPRRAHPAPPQPAGSAAGHSHAAQKEEKKAGVVSGTPVDA
                     YHDAQELKHDKKVAVYISVLAVLLAIAATGSNDAMKNAQQAGFQSNDHYAYYQSKYVR
                     QSQLKLANDQLQLKLDETPNVESLPEAARKLIQTKKAEYDKEIQKLETDGRHGKTELL
                     AMAESCDNQRKLALAQHPFYDYSMAMLQIAIVLASASIIMGTPLLLFGSFGVGAFGLL
                     LLLNGFTLLYGNPELKHDRADALEKAGVHISL"
     gene            complement(769..1389)
                     /locus_tag="VNR65_13370"
     CDS             complement(769..1389)
                     /locus_tag="VNR65_13370"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF002339.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HWJ19708.1"
                     /translation="MDNVLVLVRHGESEWNKLNLFTGWRDPDLTETGVEEAHRAGVLL
                     KKEALVFDIAFTSALKRAQHTLDIILDELGQQGLETIKDQAINERHYGDLCGLNKDDA
                     RKKWGEEQVHIWRRSYDVPPPGGESLKDTAARVLPYYDSTILPQVLAGKNVIISAHGN
                     SLRALIMRLDKLSPEEILKLNLPTGEPYVYRLNADGSVASKATLTA"
     gene            complement(1429..2226)
                     /gene="dapB"
                     /locus_tag="VNR65_13375"
     CDS             complement(1429..2226)
                     /gene="dapB"
                     /locus_tag="VNR65_13375"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWJ19709.1"
                     /translation="MRLAIMGAGGRMGRELIRTIHESPHCGVAGAVERPDSPFIGKDA
                     GEVAGVGPLGVKITSEALPLFTHIEGVLDFTSPATSVATAALAAQARVVHVIGTTGFS
                     ADDEAKIEAAARHATIIKSGNMSLGVNLLSALVEEAARALDERFDIEIVEMHHKHKVD
                     APSGTALLLGKAAAAGRRIALDEHSVRGRDGHTGERLLGSIGFASLRGGSVVGDHTVI
                     FAGAGERLELTHRAEDRQIFARGALAAALWGRGRDPGLFSMRNVLGI"
     gene            complement(2367..>2637)
                     /locus_tag="VNR65_13380"
     CDS             complement(2367..>2637)
                     /locus_tag="VNR65_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19710.1"
                     /translation="YLILQMTFLLFSASQIRALGVLDSVLAIAPVVAGLVLLVQTIIS
                     PTFHLSSYQFNSLAVLIVAGASEFLLTIWIRFVLNRRTIGFGGDN"
BASE COUNT          514 a          788 c          773 g          562 t
ORIGIN      
        1 ttgagcgaga tgtgaactcc ggccttctcc agcgcatcgg ccctgtcgtg tttcagttcc
       61 gggttgccat aaaggagggt gaagccattc agtaaaagca ggagaccgaa ggctcccacg
      121 ccgaagctcc cgaacagcag cagtggcgtt cccatgatga tggaagcgga agccagcacg
      181 atcgcgattt gcagcatcgc catcgaatag tcgtagaatg gatgctgcgc gagcgccaac
      241 ttgcgctggt tatcgcaaga ttcggccatc gccagaagtt ccgtcttgcc gtgccggcca
      301 tccgtttcaa gcttctggat ttccttgtcg tactcagcct tttttgtctg gatgagtttg
      361 cgcgccgcct ccggaaggct ttctacgttc ggcgtctcgt cgagtttcag ctgcaactgg
      421 tcgttagcga gcttgagctg cgactgtcgg acgtatttcg attgataata ggcgtagtga
      481 tcattgctct gaaaaccggc ctgctgggcg tttttcatcg cgtcgttgct gccggtcgcg
      541 gcaatcgcaa gcaggaccgc aagcacggaa atatagactg ctactttctt gtcgtgctta
      601 agctcctgcg catcgtggta agcgtccacg ggcgtgccgc tgacgacgcc cgctttcttt
      661 tcttcctttt gggcggcgtg cgaatgcccg gcagcactgc ctgcgggctg cggtggcgcc
      721 ggatgcgcac gtcttggctg attctttttc atgcccctaa gcccgcgctt atgccgtcag
      781 agtggctttg gacgcgaccg aaccgtcggc gttcagccgg tacacgtaag gctcgccggt
      841 gggcaggttg agtttcaaaa tctcttcggg cgacaatttg tcgagccgca tgatgagcgc
      901 gcggagcgag ttgccgtgcg ccgagatgat cacattcttt ccggccagaa cctggggcaa
      961 tatcgtgctg tcgtaatagg gaaggacgcg cgcggcggta tccttgaggc tctctccgcc
     1021 gggaggcggc acgtcatagg accggcgcca gatgtggacc tgttcctcgc cccacttttt
     1081 gcgcgcgtca tccttgttca agccgcacag atcgccatag tggcgctcgt tgattgcctg
     1141 gtccttgatg gtttccagac cttgctgacc caactcgtcc aggataatat cgagcgtgtg
     1201 ctgagctctc ttgagcgcgc ttgtaaaggc tatgtcgaac accaacgctt ctttcttgag
     1261 aagcacgcca gccctgtgag cttcctcaac gccggtctcg gtgagatccg gatcgcgcca
     1321 gccggtaaag aggttcagct tgttccattc gctctcgccg tggcgaacaa gcacgagcac
     1381 gttgtccatt ttctctatcc tcgctttacg caaatcggct taggcttatc agatcccaag
     1441 cacgttccgc atcgaaaaca ggccgggatc tcgccctcgg ccccaaagtg ccgcagctaa
     1501 agcgccgcgg gcgaatatct ggcgatcttc ggcgcgatgg gtcagttcca gtctctcgcc
     1561 agcccccgcg aaaatgacgg tgtgatcgcc gacgacgctt ccgcctctga gcgatgcgaa
     1621 gccgatggaa cctaagagcc tctccccggt gtggccgtcc ctgcccctga cgctgtgctc
     1681 gtcgagcgca atgcgacgcc ctgcagcagc ggccttgccg agcaaaagcg ccgttcctga
     1741 aggcgcatcg accttgtgct tgtggtgcat ttccacaatt tcgatgtcga aacgctcatc
     1801 gagcgcgcgc gcagcctcct cgacgagcgc cgaaagaagg ttcacgccga ggctcatatt
     1861 gccggatttg atgatcgtcg catggcgcgc cgccgcttcg attttcgctt cgtcgtcggc
     1921 ggaaaacccg gtcgtgccga taacatggac gacgcgggcc tgcgcggcga gagcagcggt
     1981 cgcaacgctc gtcgccggag acgtaaagtc cagcacccct tcgatatggg tgaagagcgg
     2041 aagcgcctcg ctcgtgattt tcacgccgag aggcccgacg cccgcaacct cgccagcatc
     2101 cttgccgata aagggagagt ccggcctttc aacagcaccg gctacaccgc aatgaggcga
     2161 ttcatgaatg gtgcggataa gctcgcgccc catccggccg ccagctccca tgattgctag
     2221 ccgcatgacc tacctcgact gtgtccctgc cgacgaccgt tcagagagca ttcacccatg
     2281 atccagagtg ccagaactgc agttgccgga gaaggcttta aaagcctttc cggcattgtt
     2341 tcgggcacag ccaaatgatg agccgcttag ttgtcgccgc cgaagccgat ggttcgacgg
     2401 ttgaggacga acctgatcca gatcgtcagg agaaactccg acgctcccgc gacaatgaga
     2461 accgcgagcg aattgaactg ataagatgac aagtggaagg tggggctgat gatcgtctga
     2521 acgagcagca ccaagccagc gaccacggga gcgatggcca gcaccgaatc gaggacgccc
     2581 agcgcccgga tctggctcgc ggagaacagc agaaaggtca tctggagtat caggtag
//
LOCUS       DATMGX010000815         2338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_176064, whole genome shotgun sequence.
ACCESSION   DATMGX010000815 DATMGX010000000
VERSION     DATMGX010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176064"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1856)
                     /locus_tag="VNR65_13385"
     CDS             complement(153..1856)
                     /locus_tag="VNR65_13385"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19711.1"
                     /translation="MMIGSVMASAGEQLATAVPDRAHLQKMTARFAPTELNVDISNLS
                     EGDRKALVRLVEAARVIDDVYMTQYWSGDLALYAKLQKDTSPLGKARLHYFWINKSPW
                     SELDGSTAFIPGVPEHKLPGANFYPETMTKEQFENWVKTLPEKQQEQAKGFFTIIRSD
                     GKSGFKIVPYNEAYKTDLTRAANLLKEAASLTDNTTLKRFLNTRAAAFLSNDYYESDV
                     AWMDLDAPLDITIGPYETYNDELFGYKASYEAYVNLRDEEESAKLGAFSKNLQEIENH
                     LPIDPKYRNPKIGGQAPIRVVNEVMSAGDGNHGVQTAAYNLPNDERVVAEKGSKRVML
                     KNVQQAKFDKTLIPISRLVLGGDEQDRVEFEAFFTHILTHELCHGLGPHQITVNGKPT
                     TPREQLKELYSAIEEAKADVTGLFALQYMLDHAQAMGLADVLKPNRASEKRLYATYLA
                     SSFRTLRFGLNDAHGKGMAIQVNYLMDKGAFVMRPDETFAVDYAKIKDAVRDLTHDLL
                     TVEAEGNYAGAKKMLDTLGVLRPEFKRALDKLGDIPVDIEPIFVTAEKIAPHTTAPHR
                     Q"
BASE COUNT          439 a          680 c          678 g          541 t
ORIGIN      
        1 aatgttttcg caggatccaa aatgaagtac ctcagcggct gaagccgctt gtcatcgatt
       61 ccgctcaacg cgggcctgaa ggcccgcact acccgagcag tgttcaaaat ttttccgcag
      121 tctgttcagc cgtattcttt caaacaactt gttcactgcc ggtgcggcgc cgtggtgtgt
      181 ggcgcaatct tctccgcggt cacgaagatg ggctcgatgt ccaccggaat gtcgcctagc
      241 ttgtcgagcg cccgcttgaa ctcgggacgc agcactccca aggtgtcgag catcttcttg
      301 gcgccggcgt aattcccttc cgcttccacc gtcagcaggt catgggtcag atcgcgcacc
      361 gcgtctttga tcttcgcgta gtccacggcg aaggtttcat cgggacgcat cacgaacgcg
      421 cctttgtcca tcaggtaatt cacctggata gccatgccct ttccgtgcgc gtcgttcagt
      481 ccaaagcgca aggtgcgaaa cgacgatgca agatacgtgg catacaagcg cttctcggaa
      541 gcgcggttcg gcttcagaac atcggccagg cccatggcct gagcgtgatc gagcatgtac
      601 tgcaacgcga acagtcccgt aacgtcggcc ttggcttcct cgatcgcgct gtagagttcc
      661 ttcaactgct cgcgcggcgt agtcggcttt ccattcaccg tgatctggtg cggccccagt
      721 ccgtggcaaa gctcgtgggt caggatgtgg gtgaagaagg cctcgaactc gactctgtcc
      781 tgctcgtcgc cgcccagcac cagcctggag atcgggataa gcgtcttatc gaactttgcc
      841 tgctgcacat tcttcagcat gacccgcttg ctgcccttct ccgcgaccac gcgctcgtcg
      901 ttgggcaggt tgtaggcggc ggtctgcacg ccgtggttgc catcgcctgc gctcatcact
      961 tcattgacca cgcggatggg cgcttgtccg ccgatcttcg gattgcgata cttcggatcg
     1021 atcggaagat ggttttcgat ctcctgcaaa ttcttggaga acgcgcccag cttggcgctc
     1081 tcctcctcgt cgcgcaggtt gacgtaggct tcgtacgacg ccttatagcc gaacagctcg
     1141 tcgttgtacg tttcgtatgg cccgatggta atgtcgagcg gcgcgtcgag gtccatccac
     1201 gcaacgtcgc tctcgtagta gtcgttggag aggaaggccg cggcgcgcgt gttcaggaag
     1261 cgcttcagcg tcgtattgtc ggtgagcgac gccgcttcct tcaacaggtt ggcagcgcgc
     1321 gtgaggtcag tcttgtaggc ttcgttgtag ggaacgatct tgaatccgct cttgccgtcg
     1381 gagcgaatga tggtgaagaa tcccttggcc tgctcctgct gtttctcggg cagcgtcttc
     1441 acccagtttt cgaactgctc cttggtcatg gtctcgggat agaagttcgc acccggcagc
     1501 ttgtgttccg gtacgccggg aatgaatgcc gtgctgccgt ccagctccga ccacgggctc
     1561 ttgttgatcc agaagtagtg caggcgggcc ttacccagcg gagaagtatc tttctgtagc
     1621 ttggcgtaga gcgccaagtc gccgctccaa tactgcgtca tgtaaacgtc gtcaatgacg
     1681 cgcgccgcct ccaccagcct gaccagcgct ttgcggtcgc cctcggagag attgctgatg
     1741 tccacgttca actccgtggg agcgaaacgc gcggtcatct tctgtagatg cgcgcggtcg
     1801 ggaactgcgg tggcaagctg ttcaccggcg gatgccatga cggagccaat catcattatt
     1861 aacgcgacaa aggcaaggtg cttcacggga tgagtctcct gcgttcgcgg tgggtgcacc
     1921 ggcaagacgc gccgccgcga acaatgcaaa ctgatgatga taacgcaagg cagagggcga
     1981 cgcagggggg cgcattcgcc tccaaccgta tccggcgatc ccgaagctga gtgaagaaac
     2041 aatgtcaaaa ctgcaatctc agcgttcttt accaccgatt cagtgctaaa gttctgtcgc
     2101 cgcctcgtct gagcaaatct ttgtcttcaa gctcgaagag atttttgaat cgaggagatc
     2161 gaaaatggga cgcaagaatt tctgctactt catcagtcgg cctgctgcgg tttttgcact
     2221 ggttgcaatg ctgctcagca gcgcctgggc ggccaccgag aagccgcttt atagctttac
     2281 cggcgggaat gacggaggcg atccggcttc gcgattgacc ttcgatagct caggcaat
//
LOCUS       DATMGX010000816         2465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_48521, whole genome shotgun sequence.
ACCESSION   DATMGX010000816 DATMGX010000000
VERSION     DATMGX010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48521"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /gene="creD"
                     /locus_tag="VNR65_13390"
     CDS             complement(<1..1228)
                     /gene="creD"
                     /locus_tag="VNR65_13390"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HWJ19712.1"
                     /translation="MAASGFFANLVRSPSFKFVLVGVLVLLISALTGVVWLLSAERQS
                     RANAVQAEIAHDWGGNQRILGPYLIVPYTLKAQRLVDGKAVDIEVTRQAVFLPDTLNV
                     SGNVQSQMRHRSIYDVTVYQGKLTLEGQYRPADLSLVEPETPASIRWQDAFIALGIQD
                     VSGLKNNVQLQIGGQKIDFEPSAGLADEAGRVNGIHARLKPLAVAGGLNGRAPLPYKI
                     ELELNGSSSLAFAPAGRENTVALDSNWPHPGFTGFLPVSRTITGQGFSANWSVPYLAR
                     DVPQAWAGVPQGIVLDRFMGKDLGVNLFVPVDFYALVERALKYGFLFVAAAFGGMFVL
                     ELLSGRRVHPIQYIFVGLAIVIFYVLLLSLSEHIGFSPAYLAASVATGAMISTFVGMA
                     MQSLRRGLTMLGIFGVL"
     gene            2016..>2465
                     /locus_tag="VNR65_13395"
     CDS             2016..>2465
                     /locus_tag="VNR65_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWJ19713.1"
                     /translation="MPELPEVEIIRRGLVPAMEGASFDAVTLNRPDLRFPFETDFAKR
                     LLSERVSRLTRRAKYIVVETESGLGLAMHLGMTGRFTIQHNAVGHAETPGAFYYDYDA
                     NPRHDHVVFGMSNGDVIRYNDVRRFGSMSLFEAKGMAEHPLFKALGVE"
BASE COUNT          572 a          729 c          687 g          477 t
ORIGIN      
        1 acagcacgcc gaaaatgccg agcatggtaa gaccgcgcct caagctttgc atcgccatac
       61 cgacaaaggt ggaaatcatg gcaccggtcg ccacagaagc ggcgagatag gcgggcgaaa
      121 agccgatatg ctccgacagc gagagcagaa gcacgtagaa gatcacaatc gcgaggccca
      181 cgaagatata ttggatcgga tgtacgcgcc gcccgctgag cagctcaagc acgaacattc
      241 cgccaaatgc cgccgccacg aacagaaagc cgtacttcag cgcacgctcg accagcgcat
      301 agaaatccac cggcacaaac agattcacgc ccaggtcttt gcccatgaac cggtcgagca
      361 caattccttg cggaactccg gcccaggctt gtggcacatc gcgcgcaaga tagggaacgc
      421 tccaattggc cgaaaacccc tggccggtaa tggtccgcga caccggaagg aagcccgtga
      481 agcctggatg cggccaattc gaatccagcg cgacggtatt ctcacgaccc gcaggtgcaa
      541 aggcgagcga agatgagcca ttcagctcca gctcgatctt gtaaggcagc ggcgcccttc
      601 cattcagtcc gccagcaacc gcaagcggtt tcagccgcgc gtgaatgccg ttgaccctcc
      661 ctgcctcatc ggcaaggccg gcgctcggct cgaaatcgat cttctgcccg ccgatctgaa
      721 gctgcacatt gttcttgagc ccggaaacgt cttggatgcc gagcgcgatg aacgcatcct
      781 gccagcgaat ggaggccggc gtctccggct caacgaggct tagatccgcc gggcggtatt
      841 gcccttccaa cgtgagcttg ccctgataaa ccgtcacatc gtagatcgag cggtgccgca
      901 tctgcgattg cacgttaccg cttacgttca gcgtatccgg caggaacacg gcctgccgcg
      961 tcacctcaat atcgacggcc ttgccatcaa cgagccgttg cgcctttagc gtatagggca
     1021 cgatcaaata cgggccgaga atgcgctgat tgccacccca atcatgggcg atttcggcct
     1081 gtacggcgtt ggccctgctc tggcgttcgg cgctcaacag ccaaaccacg ccagtgagag
     1141 ccgagatgag caggacgagc acgccaacga gcacaaattt aaacgacgga gagcggacaa
     1201 ggttggcaaa aaatcccgat gcagccatgg ggcgatcctt tgcagaagtt tcccttccat
     1261 ggagggcggt gcaagcaggc aaatgtaagt ctagaatccg cctggatcgc ggccgaattt
     1321 gccctcgggc cgcaaagaga cactcagcaa caattggggg agcgcctgct atctgggttg
     1381 cccagacgca ccagatcggc gcgcgctgtt tgaggctcgc cgcgtgctcg ccggtagaca
     1441 gttcgttatc gcgtccggcg ttaatacaaa ggcagttaac aggagtgcgc agtatttatt
     1501 tccagtcacc aatatgctta acaaccattt tatccctgcc acaattactt cgcgccgagc
     1561 ctgcttcccg gccggaagta aatccgataa gccattgata tccaaaaagt atccgtcgcc
     1621 catgacctcc cattaaatag aatttaatgc cggaattatt tcatttactg ggacgagcaa
     1681 aagtccgtta attatgtttt ggcttcgcga attaacgctt gcgctgagcc ggatcggctg
     1741 cggggcaggc gcacgatggc gtctcgttcc gtatttttcc ccatgtttat caagggttgg
     1801 agcgggctct cgcttccgtt aacccgccgc aaaggcgagt tcgctgaaga tctttcatag
     1861 gaaggaaggc ggacgaaagt gtaagggaaa agttgacatg tggatgccga taattgcgag
     1921 atgcatacaa aatgaagacc tggacgcccg cgaccgatca tgtcacgacg ccttaaagcc
     1981 cttgccaagc atcggcgttg tggcaagagt ctgccatgcc tgaactgccc gaagtcgaga
     2041 tcatccggcg aggcctcgtc ccggccatgg aaggcgcttc attcgatgcg gttacgctta
     2101 accgccccga tttgcgcttt cctttcgaaa cggatttcgc aaagcggcta ttgagcgagc
     2161 gcgtgtcccg cctcacccgc cgcgccaaat acatcgtcgt ggaaacggaa agcgggctgg
     2221 gcctcgcgat gcatctcggc atgaccggac gcttcacgat ccagcacaac gcagtcgggc
     2281 acgccgagac gccgggtgca ttttattacg attacgatgc caatccgcgc cacgaccatg
     2341 tggtgttcgg catgagcaat ggcgacgtga tccgttacaa cgatgtccgg cgcttcggtt
     2401 ctatgtcgct tttcgaagcg aagggcatgg ccgagcaccc gctcttcaaa gcgcttggcg
     2461 tcgag
//
LOCUS       DATMGX010000817         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_185648, whole genome shotgun sequence.
ACCESSION   DATMGX010000817 DATMGX010000000
VERSION     DATMGX010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185648"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VNR65_13400"
     CDS             <1..402
                     /locus_tag="VNR65_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWJ19714.1"
                     /translation="RLGIVRPRIAVAGLNPHSGENGLFGTDEIERIKPAIEAARSEGI
                     VVTGPISGDTVFYRALHGEFDLVVAQYHDQGHIPTKLIAFDTTINVSLGLPILRVSVD
                     HGTAFDIAWQGVARHENMNATIAYARRLASS"
     gene            442..1677
                     /locus_tag="VNR65_13405"
     CDS             442..1677
                     /locus_tag="VNR65_13405"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HWJ19715.1"
                     /translation="MTHRRLDVAIVADDLTGALDAAAPFAGRGLETRILLDFEQAAAA
                     VETKTRVLSLTSESRHLSPTAADERVWNATRAALAGAPRILLKKIDSTLRGNIAAEIM
                     AGLRATGLQHALIAPAVPRHGRIMREGEVFMNGVPLRQTEIGADALSPSPSATLPQIL
                     RAASKDLIVHVWTRGAAFALNAEAGIHAYVADCETEADLDAIAQFVISHCDEVLPVGA
                     SGLATAIARQLAHTPSSELLSVIQRISEQAQNPILFVIGSRTAASAEQAARLCAAGAE
                     ELRMSFSAHTRDGDWILDRSVNESTPPVALIVRPESSRESNLISASEVAQALGRTAAD
                     IVRRLQVDAVVMIGGDTAFETFQALGVSEASVAGEIMPGVAIGTMQVDGRPLTFITKA
                     GGFGDANALVEIIRYLKNQ"
BASE COUNT          444 a          570 c          566 g          426 t
ORIGIN      
        1 cggcttggca ttgttcgccc gcgcatcgcg gtcgccggat tgaatcctca tagcggagag
       61 aacgggctgt tcggcaccga tgaaatcgaa cgaataaaac cggctatcga agcggcacgc
      121 agcgaaggca tcgtagtgac agggccgatt tccggcgata cggtgttcta tcgtgccctg
      181 cacggtgagt ttgacctggt cgtcgcgcaa tatcacgacc agggtcacat cccgaccaag
      241 ctgattgcgt tcgataccac cattaacgta agcctcggcc ttccgatctt gcgcgtatcg
      301 gtcgatcatg ggaccgcgtt tgacattgcc tggcagggcg tcgcgcgtca tgaaaatatg
      361 aatgccacga ttgcctacgc gcggcggctc gcaagttctt aaccgcccaa aacgaaaacg
      421 ccaagccgat cgggcttcga tatgacgcat cgtaggctcg acgttgccat cgtcgccgat
      481 gatctcacgg gagccctgga tgctgcagcg ccgtttgcag ggcgcggcct tgaaacacgt
      541 atattactcg attttgagca ggccgcggct gctgtcgaga ccaaaactcg ggtattgtcc
      601 ttgaccagcg aaagtcggca tctctcgcca accgcagccg acgagcgcgt ctggaatgcg
      661 acacgtgctg cgcttgcagg tgcgccccga attttattga aaaaaataga ttccactctg
      721 cgcggcaaca ttgccgccga gattatggcc gggcttcgtg cgaccggact gcagcatgcc
      781 ctgatcgcac cagctgttcc caggcacggc cgcatcatgc gcgaaggcga ggtgttcatg
      841 aatggcgtgc cacttcggca gacggagatc ggggccgacg ccctatcgcc gtcgccttca
      901 gcgacgctac cgcaaatctt gcgagcagcg tcgaaggacc tgatcgtcca tgtgtggacg
      961 cgcggcgcgg ccttcgcgct caacgccgaa gccggaattc acgcctacgt cgccgattgc
     1021 gagaccgagg ccgatcttga tgccatcgcc caatttgtca taagccactg cgacgaagtc
     1081 ctacccgttg gagcgtcagg tcttgctaca gcgatagcca ggcagctcgc gcatacaccg
     1141 tcatcggaac ttttgtcagt aatccagagg atcagcgagc aagctcagaa tcctattctc
     1201 tttgtcatcg gctcgcgaac cgcggcgagt gccgaacaag cggcgcgatt gtgcgcagcc
     1261 ggcgccgagg aactgagaat gtctttttcg gcgcacacac gagatggcga ttggattttg
     1321 gatcgcagcg tcaacgagag tacacctccg gtagcattaa ttgtccggcc agagtcgtcg
     1381 cgagagtcaa atctaattag tgcttccgaa gttgcacaag cacttggacg cacggcggca
     1441 gacatagtcc gccgtctgca agtagatgcg gtcgttatga tcggagggga taccgcgttc
     1501 gagaccttcc aagcccttgg cgtttccgaa gcctcggtag caggagaaat tatgccaggt
     1561 gtcgcgattg ggacaatgca ggtagacggg cgcccgctga ctttcattac caaagccggt
     1621 ggatttggtg acgcgaacgc actagttgag ataattagat atttgaaaaa tcagtaaatc
     1681 gcgccgcgca aatagacttg cgccggcggc cattgcggcg cgatggctgt atcgggtctt
     1741 agccgattct gttccgtagc cttccattct gcgaatcgaa tcccaatcag ggatacccca
     1801 gataatttgc tgtatgcgct ttttaatcgc gaaacaaact gacttttcga cgctgcatcg
     1861 ttcgctcaaa gctgagcaag cggtgcaagc acatcggcgc atttaagcgt taacctatgc
     1921 tagcgagcat ccattgttct tctccactac gcctatccag gccggataga attttcggaa
     1981 ggaacaatca gagccggcac catcta
//
LOCUS       DATMGX010000818         3871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_430515, whole genome shotgun sequence.
ACCESSION   DATMGX010000818 DATMGX010000000
VERSION     DATMGX010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430515"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VNR65_13410"
     CDS             <1..971
                     /locus_tag="VNR65_13410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWJ19716.1"
                     /translation="SIGAIAGGAATGSSDTAQAGTAMMFGGQQVLMRSILSYRRAQES
                     SADQAGVDYLTKSHQSPAGMLKTFRYFADQSVGTLKVDPYIQSHPVPMERIAQLQEIA
                     QRSPYLNEKDTPQLQMRHDLVRAKIYAYTYKNNPQRVLRQYSDNGLPARYARAIVKYH
                     GGGYEAAAPALDELISANPQNPWFYELKGTFLMESGNAKDAVGPFRKAVSLVPKSGFM
                     RIELAQAILESGGGTADEALKLLRVALIDEDQSYLGYRLQSQAYGKLKRYPEADLSSA
                     LASFHQGDVKNAKMLAERAKDGLPSGTPGWVKADDIVNFKMEKVRG"
     gene            1021..1845
                     /locus_tag="VNR65_13415"
     CDS             1021..1845
                     /locus_tag="VNR65_13415"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWJ19717.1"
                     /translation="MKKFTKVAATVLMAAGLTLALSRGGVPAMAEADSFSEGQVKSIE
                     KIVKEYLISHPEIMLEVQEAYEKKVEAKRGEATRSRMPEFYGALSSMNGELAPLSIGS
                     GDVTVVEFFDYNCGYCRQTLPELIKLIDSDKKIKVQFMEYPILAPGSTEAAKVAIAAA
                     KQGKYLEFHKAMFAAGRANKESALKVAEQIGLDMEKVKADSASPETEALVTKISELGK
                     RIFIDGTPTFIVGDKTNPGAADYDQLKQLVDETRKDGCKACAEATAPAGVKDEKKS"
     gene            2018..2488
                     /gene="aroQ"
                     /locus_tag="VNR65_13420"
     CDS             2018..2488
                     /gene="aroQ"
                     /locus_tag="VNR65_13420"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWJ19718.1"
                     /translation="MKPIFILNGPNLNLIGDREPEIYGVGTLAGIEKGLREQASAAEL
                     EIVFRQTNHEGVLVDWIQEARHASSALILNAGAYTHTSIAVMDALRALSIPIAEVHLS
                     NIFKRESFRHHSYVSPAATGVICGFGAQSYRLALAAMIEIVKSSSAASNRGLHA"
     gene            2481..2966
                     /gene="accB"
                     /locus_tag="VNR65_13425"
     CDS             2481..2966
                     /gene="accB"
                     /locus_tag="VNR65_13425"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HWJ19719.1"
                     /translation="MPEPAKKSMPESAQKKSASEDLIRSLAELLNETGLSEIEIEENN
                     VRVRVARTLMMPTHLMHSPYAGPATAGVSSTNSAASLENHPGVVTSPMVGTAYRAPEP
                     GAPFFVEVGSVVREGDTLLIVEAMKTMNQISAPRAGTVKRVLVENGQPVEYGEPLMII
                     E"
     gene            2975..>3871
                     /locus_tag="VNR65_13430"
     CDS             2975..>3871
                     /locus_tag="VNR65_13430"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWJ19720.1"
                     /translation="MFDKVLIANRGEIALRIERACKELGIATVAVHSTADADAMHVRL
                     ADESVCIGPPAASESYLNIPRLLAACEITGADALHPGYGFLSENARFAEILEEHGVTF
                     IGPTPEHIRLMGDKIRAKETAKKLGIPVVPGSGGAVTSDAEAARIARDIGYPVLVKAA
                     AGGGGRGMKVARTAEDLSLALSTARAESKAAFGDDALYLERYLMRPRHIEVQVIGDGK
                     GNAVYLGERDCSLQRRHQKIMEETPSPALNEAQRRGIGETVANAMRTLNYRNAGTVEF
                     LYEDGEFFFIEMNTRLQVEHPVT"
BASE COUNT          875 a         1079 c         1129 g          788 t
ORIGIN      
        1 tgagcattgg cgcgattgcc ggtggcgcgg cgacgggaag cagcgacacg gcgcaagcgg
       61 gaactgccat gatgttcggc ggccagcaag tccttatgcg ctcaattctg tcctaccgcc
      121 gcgcgcagga atcgtccgcc gatcaggctg gcgtggacta tctgaccaaa tctcatcaat
      181 cgccggccgg aatgctgaag acgttccgct acttcgcgga tcagtcggtg ggtacgctta
      241 aggtcgatcc ttatattcaa tctcaccctg ttccgatgga acgcattgcg caactgcagg
      301 agatcgcgca gcgcagccct tacctcaatg aaaaggacac gccgcaactg cagatgaggc
      361 acgatctcgt ccgcgcgaag atttacgctt acacctacaa gaacaatcca cagcgggtcc
      421 tgcggcaata ttccgacaat ggactgcccg cgcgttacgc ccgcgccatc gtgaaatatc
      481 atggcggcgg ctatgaggct gcggctccag cgctcgacga actcatatcc gcgaatcccc
      541 agaacccttg gttttatgag ctgaagggta catttcttat ggagagcgga aatgcgaagg
      601 atgccgtcgg gccgtttcga aaggctgtgt cgctcgtgcc gaagtcggga ttcatgcgta
      661 tagagcttgc acaggccatc ctcgaaagtg gcggcggaac tgcggatgaa gcgctcaagc
      721 tgcttcgtgt cgcccttatc gacgaggatc aatcctacct cggctatcgc cttcaatctc
      781 aggcttatgg gaaactgaag cgctatccgg aagccgacct ttcgtcagcg ctggcaagtt
      841 ttcatcaagg cgacgtcaaa aatgcaaaaa tgttggcgga gcgtgctaaa gacggcctcc
      901 catcggggac tcccgggtgg gtcaaggctg atgatattgt gaacttcaaa atggaaaaag
      961 tgcgtggtta gacgcttcga actcagctaa cccgttggcg atcggaattc cggaggaaat
     1021 atgaaaaaat ttactaaagt ggcggccaca gttctcatgg ccgcgggctt gaccttagcg
     1081 ctctctcgcg gcggagttcc agcgatggcg gaagcggata gcttcagcga agggcaggtg
     1141 aagtcgatcg aaaagatcgt caaggagtac ctgatcagtc accctgagat catgctcgaa
     1201 gtccaggagg cctacgaaaa gaaggtcgag gccaagcgcg gcgaggcgac ccgctcccgc
     1261 atgccggaat tttacggcgc cctgtccagc atgaacggtg aactggcgcc gctgagcatc
     1321 ggcagtggag atgtcaccgt cgtcgagttc ttcgactata attgcggcta ttgccggcaa
     1381 acccttccgg aactcatcaa gctgatcgat agcgacaaga agatcaaggt gcagttcatg
     1441 gagtacccga tcctggctcc agggtcgacg gaggcggcaa aggttgccat cgccgcagcc
     1501 aagcaaggca aatatcttga atttcacaaa gcgatgtttg ccgctggccg cgcgaacaaa
     1561 gagagtgcgc tcaaggttgc cgagcagatc ggtctcgaca tggagaaggt gaaggcagat
     1621 agcgcctcgc ccgaaaccga ggctctggtc acgaagattt ccgagctcgg taagcgcata
     1681 tttattgatg gaacgccgac attcatcgtc ggtgacaaga caaatccagg cgctgccgac
     1741 tacgatcaac ttaagcagtt agtagatgaa acacgcaaag acggctgcaa agcttgtgcg
     1801 gaagccaccg ctccggccgg tgtaaaagac gaaaaaaaat cctgacgctt tttgcactgc
     1861 gtcataaaaa ctggtaacag agagccacag ccggttcggt gtggcttgcc cgttttggcg
     1921 gcacgctgag ccatgctttc caatgaggca tacctcagct tccgtgaggg ctgtgggatg
     1981 tctctgtttc caccggaaaa accttgcatt cgtgtcaatg aagccgatct ttatcctcaa
     2041 tggccctaac ctgaatctca ttggcgaccg ggagcctgag atttacggcg tcggcacgct
     2101 ggccggcatc gaaaaaggcc ttcgagagca agcgtccgcg gcggagttgg agatcgtttt
     2161 ccgtcagacg aaccacgaag gtgtgcttgt cgactggatt caggaagcgc ggcacgcgtc
     2221 gtcggcgctc attttaaacg caggggccta cacgcacacc tctattgctg tcatggacgc
     2281 gctgcgggct ctttcaattc ctatagcaga ggtccacctt agcaacatct tcaagcgcga
     2341 gtcgttccgg catcactctt atgtctcgcc cgccgcgacc ggtgtaatct gcggatttgg
     2401 tgctcaaagc taccgtttgg cgctcgctgc catgatcgag attgtaaagt cttcttctgc
     2461 tgcttccaac cggggtcttc atgcctgaac cggccaaaaa atcgatgcct gaatcggcgc
     2521 aaaaaaaatc agccagtgag gatctcatcc gtagcctcgc tgaattgctg aatgagactg
     2581 gactttccga gatcgagatc gaggagaaca acgtgcgtgt gcgtgtggcg cgtacgctta
     2641 tgatgccgac gcatctgatg cattcccctt atgcagggcc cgcaacggca ggggtctcct
     2701 ccacgaattc cgctgcaagt ttggagaacc atcccggcgt tgtgacatcg cccatggttg
     2761 gcacggcata tcgcgctccc gagccgggtg cgccattttt tgttgaggtc ggttccgtgg
     2821 tccgcgaggg cgatacgctg ctgatcgtcg aggcaatgaa gacgatgaat cagatctctg
     2881 cgcccagggc aggcacggtt aaacgtgtcc ttgtggaaaa tggtcagcct gtcgagtacg
     2941 gggaacccct catgataatc gaataggcgc ggccatgttc gataaggtcc ttatcgccaa
     3001 tcgcggagag atcgctcttc ggattgagcg cgcctgcaaa gagctgggca tcgcaaccgt
     3061 cgccgtccat tccaccgcgg atgccgatgc catgcatgtc cgcctcgccg atgaaagcgt
     3121 ttgcatcggc cccccggcag cgagcgaaag ctatctgaac attccacggc tgctggcggc
     3181 ctgcgaaatc acgggcgcgg acgcgttaca tcccggctac ggttttctgt ccgagaacgc
     3241 gcgcttcgcc gaaattctcg aagagcacgg ggttacgttt atcgggccca cccccgaaca
     3301 tatcaggctc atgggcgata agatccgggc gaaagagaca gcaaaaaagc ttggtatccc
     3361 ggtagtccct ggctctggcg gggccgtcac cagcgacgca gaggccgcca gaatagcgcg
     3421 tgatatcggc tatccggtcc tggtcaaggc ggcggcaggc ggcggcggtc gcggcatgaa
     3481 agttgcccga acggcagagg atttgtcgct cgcgctgtct acggcgcgtg cggaatccaa
     3541 ggcggccttc ggcgacgacg cgctttatct cgaacgctat ctcatgcggc cgcgccacat
     3601 cgaggttcag gtcatcggag atggcaaggg aaacgccgtt tatctcggcg aacgcgattg
     3661 ctcccttcaa cgccgccacc agaagatcat ggaggaaacg ccgtcacccg ccttgaatga
     3721 ggcgcagcgc cgcgggatcg gcgagaccgt cgccaatgcg atgcgcacgt tgaattaccg
     3781 caacgccggc accgtcgagt ttctttacga agatggggaa ttcttcttca tcgaaatgaa
     3841 cacgagactt caagtcgagc atccggttac c
//
LOCUS       DATMGX010000819         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_389833, whole genome shotgun sequence.
ACCESSION   DATMGX010000819 DATMGX010000000
VERSION     DATMGX010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389833"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1529)
                     /locus_tag="VNR65_13435"
     CDS             complement(195..1529)
                     /locus_tag="VNR65_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWJ19721.1"
                     /translation="MSQTKTALPNELDLSAYWMPFTANRQFKAHPRIFVAAEGMHYTS
                     ADGRKILDGVAGLWCVNAGHGRKEIAQAVERQLETLDYATAFQMGHPGEFELAARLAR
                     IAPEGLDRVFFVNSGSEAVDTALKIALAYHRARGEGQRTRFIGRERAYHGAGFGGMSV
                     GGLMNNRRAFGSMLPAVDHLPHTVDIARNAFSKGLPEHGAELADHLERLIGLHGADTI
                     AAVIVEPMSGSAGVLLPPKGYLEKLRAICDRHGLLLIFDEVITGFGRLGTPFAAQFFG
                     VTPDMMTVAKGLTNGAIPMGAVAAKRGIHDALMVGPEHLIELFHGYTYSGHPVACAAG
                     LATLDIYEREGLFERAASLSPLWEEAVHTLRGSPHVIDVRNLGLVAGIELEPRPSEPG
                     KRAYEVLLDCFENGVLVRQAGDILALSPPLIIEEGQIHHIVDGLAAALRRVH"
     gene            1607..2335
                     /locus_tag="VNR65_13440"
     CDS             1607..2335
                     /locus_tag="VNR65_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ19722.1"
                     /translation="MKANDAKVAIITGAGHGIGRACAGELTPLGWSCVCADVDPAQAE
                     ETAASAEGRAITCDVSKEADVKRLIEETAGTFGRIDAIISNAGIMDRKPLSETTLEDW
                     NKVIATNLTAAFLLAKFGEQHLRRSKGGIVLIASTRAHMSEPNTFAYSASKGGLAALT
                     HSLALTLGPDVRVNCISPGWINVDENAKLSAEDHAQHPAGRVGTPSDIAAAVVFLLSE
                     AAGFMTGAELIIDGGMTRKMIYVE"
     gene            2344..>2534
                     /locus_tag="VNR65_13445"
     CDS             2344..>2534
                     /locus_tag="VNR65_13445"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HWJ19723.1"
                     /translation="MRIDLNADMGEGYGAYRIGRDEDLLHIVTSANVACGFHGGDATI
                     MHKLAALAKKHGVGLGAHPG"
BASE COUNT          511 a          753 c          811 g          459 t
ORIGIN      
        1 acccctcatc ctgtccttct cccgcaaggg gagaagggac gcggttgcaa aaggtgcgac
       61 ctgccctctc tcccaaccgc atccgcttgc ggatgcggtc attttgagtt gcgaactcgc
      121 caaggcgact tcgcccggaa gagggttggg gtgaagtgga gcttagctca gcaagcctcc
      181 aatgacacgc ctcatcaatg cacgcggcgc aaagcggcgg cgagaccatc gacgatgtga
      241 tgaatctggc cttcctcgat gatgagcggc ggagagagcg caaggatgtc gcccgcctgg
      301 cggaccagaa cgccgttctc gaagcagtct agcaaaacct cgtaggcgcg cttgccgggc
      361 tcgctcggcc tgggctccag ctcgatcccg gcgacaagcc cgagattgcg aacgtcgatc
      421 acatgcgggc taccccgcag cgtatgcacc gcctcttccc acaagggaga gagcgacgcc
      481 gccctctcga acaatccctc acgctcataa atatcgagcg tcgcgagtcc ggctgcgcag
      541 gcgacagggt ggcccgaata ggtgtagccg tggaagagct cgatgaggtg ctcggggcca
      601 accatcagcg catcgtggat gccgcgtttc gcggcgaccg cgcccatcgg gattgcgccg
      661 tttgtgaggc ccttcgcgac ggtcatcatg tcgggcgtca caccgaaaaa ctgcgccgcg
      721 aacggcgtcc cgaggcgacc gaaaccggtg atgacttcat cgaagatgag gaggaggccg
      781 tgacggtcgc aaatcgcacg cagcttttca agatagccct tgggaggcaa tagaacgccc
      841 gccgatccgg acatcggctc gacgatgacg gcggcgatgg tgtccgcgcc atgcaggccg
      901 atcaggcgct ccaggtggtc ggcaagctcg gcgccgtgct cgggcaggcc tttgctgaag
      961 gcattgcggg cgatgtcgac cgtatgcggc aggtgatcca cggcgggcag catggaccca
     1021 aaggcgcggc ggttattcat gaggccgccg accgacatgc ctccgaagcc cgcgccgtga
     1081 taggcgcgct cgcgtccgat gaagcgcgtg cgctggcctt cgcctcgggc gcgatgatag
     1141 gcgagggcga tctttagcgc ggtatcgacc gcctctgagc cggagtttac aaagaagacc
     1201 cggtcaagtc cctctggcgc gatccgtgcc agacgcgccg ccagctcgaa ctcacccgga
     1261 tggcccattt gaaatgcagt ggcgtaatcg agggtttcga gttgtcgctc tacggcctgg
     1321 gcaatctcct tgcgcccatg ccctgcgttc acgcaccaaa gcccggccac gccgtcgagg
     1381 attttgcggc catccgcgct cgtgtagtgc atgccctcgg cggcaacaaa aatgcgggga
     1441 tgagctttga actgccggtt tgccgtgaac ggcatccaat aagcggaaag gtcgagctcg
     1501 ttcggaaggg cggtcttggt ctggctcatg gcatcaacct ctccagcagg ggtttgaact
     1561 tcgatacact ccatcacaac atgattggac agaaaggaaa gttggcatga aggcgaatga
     1621 tgccaaggtt gcgatcatca ccggcgcagg gcatgggatc ggccgggcat gtgccgggga
     1681 gcttacgccg ctaggatggt cctgtgtttg cgcagatgta gacccggcac aggctgagga
     1741 aacagctgct tcggctgagg gacgcgccat aacatgcgat gtctcgaaag aagcggacgt
     1801 aaagcgcttg atcgaggaaa ctgcgggcac tttcggacgt atcgatgcca tcatctcgaa
     1861 cgccggtatc atggaccgga agccgctttc cgaaacaacg ctggaagact ggaacaaagt
     1921 catcgccacc aatcttacgg cggccttcct tctcgcgaag tttggcgagc agcatctcag
     1981 gcgtagcaag ggcggcattg tgctgatcgc atcgacgcgg gcgcatatgt cggagccaaa
     2041 caccttcgcc tactccgcga gtaaaggcgg tctcgccgcg ctcacccatt cgctggcgct
     2101 cacgcttggc ccggatgtgc gcgtaaactg catctcgcca ggatggatca acgtcgatga
     2161 aaacgcgaaa ctctcggcgg aagatcatgc gcagcatccc gccgggcgcg tgggcacgcc
     2221 atccgacatc gctgctgccg tcgtttttct cctttcggag gcggcaggtt tcatgacagg
     2281 cgcggagctt atcatcgatg gtgggatgac ccggaaaatg atttacgtcg aataggaggg
     2341 gctgtgcgga tcgatctcaa cgcggatatg ggcgagggat acggtgctta tcgcatcggg
     2401 cgcgatgagg acctgctcca tatcgtgacc tcggccaatg tcgcctgcgg cttccatggc
     2461 ggcgacgcga cgatcatgca caaactcgcg gccctggcga aaaagcatgg tgtggggctt
     2521 ggcgcgcatc cggg
//
LOCUS       DATMGX010000820         2501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_113115, whole genome shotgun sequence.
ACCESSION   DATMGX010000820 DATMGX010000000
VERSION     DATMGX010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113115"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VNR65_13450"
     CDS             complement(<1..422)
                     /locus_tag="VNR65_13450"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWJ19724.1"
                     /translation="MNCAVLHLFRKSVPAGVLLIVVLLLPGMVLGQSYRGSIRGKVSD
                     PSGGLLAGAKITSKNVDTGLTREGVTNQEGTYVLAELPAGSYTVTVNAPGFAPVAQNV
                     VVSVGLDTTADFAMLKVEQHQESVTVTENAPLVEATRDV"
     gene            complement(489..1385)
                     /locus_tag="VNR65_13455"
     CDS             complement(489..1385)
                     /locus_tag="VNR65_13455"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HWJ19725.1"
                     /translation="MVVAILLVLALMTQAPVCARAQTAQKYPPTIVFMTDFGVLDDSV
                     AICRGVMYGIMPEVRIVDLTHQVTPFSIFDGARYLYGATPYFPAGTVFVVVVDPTVGS
                     TRKAIVARSKRGQYFVLPDNGLLTLVEQRDGIESVHEITNTEWMIGSKRSSTFHGRDI
                     FSPVGAHLARGDDWTKVGPEMAVKDLVRLDWKVAKLDGSGLTASVIATDGPFGNLVTN
                     VDGEDFLKLGYQRGQEVPVKVGDKELKMKFVKTFSDVPVNQSLLYIDSRGHLGLAVNQ
                     NSFAATYGIKPPATLFIPKAGK"
     gene            1647..2045
                     /locus_tag="VNR65_13460"
     CDS             1647..2045
                     /locus_tag="VNR65_13460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWJ19726.1"
                     /translation="MSQVIPEKYRDLFQKRAFASLGTLMPDGRPQVTPVWCDFDGEHV
                     IFNSAKGRQKDRNVRRDPRVSLAIVDPENPYRYLEIRGRVVEITEDGADDSINKLAKK
                     YLGVDKYPYAQPSEVRVIYKIKPEHTTVMG"
     gene            2317..>2501
                     /locus_tag="VNR65_13465"
     CDS             2317..>2501
                     /locus_tag="VNR65_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19727.1"
                     /translation="MNVDEVCGAGAPAREKPAADPNRSASQLAASTVRDWLAAAQADV
                     LFEATSLNVKDGEPAVA"
BASE COUNT          569 a          838 c          628 g          466 t
ORIGIN      
        1 acgtcccgcg tcgcctccac cagaggcgcg ttttccgtga ccgtcacgct ctcctgatgc
       61 tgctccactt tgagcatcgc gaagtcggcg gtggtatcga ggccaacact caccaccacg
      121 ttctgcgcaa ccggtgcgaa tcccggcgca ttcaccgtga cggtataact accggccggc
      181 aattccgcca gcacatacgt accttcttgg ttggtcacgc cttcgcgcgt caggccagtg
      241 tccacgtttt tcgatgtgat tttcgctccg gcaagcagcc cgccgctcgg atcgctgact
      301 ttgccccgga tcgaccctcg atagctttgc cccagaacca tccccggtag caacaaaacc
      361 acaatgagta acactccagc gggcacgctc ttgcgaaata aatgcaaaac ggcgcaattc
      421 ataatgaaaa gctcctcctg tatataaaca atggacgcat taagacgcgg tatggtgtga
      481 ctttgcgtct attttcccgc ctttgggata aacagcgttg ccggcggctt gatcccatat
      541 gtcgcggcaa aactgttctg gttcaccgcc agccccagat gcccgcgtga gtcgatgtac
      601 aacaaggact ggttcaccgg cacgtcgctg aaagtcttca cgaacttcat cttcaactct
      661 ttgtcgccga ccttgaccgg aacctcctgg ccgcgctggt atcccagctt caggaagtcc
      721 tcgccatcca cattggtcac caggttgcca aacggcccgt cggtcgcgat caccgaagca
      781 gtcagcccgc tcccgtccag ttttgcaact ttccaatcca gccggaccag atccttcacc
      841 gccatctccg gcccgacctt cgtccagtca tctccgcgcg ccaaatgcgc gcccaccggc
      901 gaaaaaatgt cgcgcccgtg aaacgtggac gaccgcttgc tcccaatcat ccattccgta
      961 ttggtaatct cgtgaacaga ttcgatgcca tcgcgctgct ccaccagcgt gagcagtccg
     1021 ttatccggca gcacaaaata ctgcccgcgc ttggagcgcg ccacgatcgc tttgcgcgtg
     1081 ctgccaacag tcggatccac cacaacaaca aacaccgtgc ccgccgggaa atacggcgtc
     1141 gccccataca ggtagcgcgc cccatcgaaa atggagaacg gcgtcacctg gtgggtcaaa
     1201 tccacaatgc gcacctcggg cataatgccg tacatcaccc cgcggcaaat ggccaccgag
     1261 tcgtcgagca cgccaaaatc cgtcataaac acgatcgtcg gcggatactt ctgggccgtc
     1321 tgcgcccgcg cgcacactgg cgcctgcgtc attaacgcca aaacgagaag gatcgcaacg
     1381 accatggccc ctcgcgacga gaggctccgg ctacggagca aagcagtcat agttcagtcc
     1441 tgaggcagaa attgatgcaa gtctacgaac ccaccgtagt tcacggcgat ggacaccgcg
     1501 agcgcagaat cagcgccccc aaaagcaggt gaatttgatt agacctgtca ttttaaacaa
     1561 agacgcaaaa aaacgggaag tgacgatggt acgcaccgcc gtgtcttgcg ttctataatg
     1621 ctgctgcaat ctaattggag gctcttatgt cgcaagtcat ccccgagaag taccgcgatc
     1681 tgtttcagaa gcgggccttt gccagcctgg ggacgttaat gcccgacggc cgcccgcaag
     1741 tcacgcccgt atggtgcgac ttcgacggcg agcacgttat cttcaactcc gccaagggac
     1801 gccagaagga ccgcaacgtc cgccgcgatc cccgcgtctc gctcgccatc gtcgatcccg
     1861 aaaacccgta ccgctatctc gagatccgcg gccgcgtcgt cgaaatcacc gaagacggcg
     1921 ccgacgacag catcaataag ctcgcgaaaa agtatctcgg cgtagacaag tatccctacg
     1981 cgcagcccag cgaagtccgc gtgatctaca agatcaaacc cgaacacacc acggtgatgg
     2041 gataggaatc gaggtttcaa ggctgcaagg tttcagagtt ccaaggttct tttggaaacc
     2101 ttgaaacctt gagaccttga aaccttcccc tcaacactcg aggagattcc catccgcacc
     2161 tacaacctct gcttcctagg ctacggcaac gtcaaccgga ctctcgtccg cctcctggaa
     2221 gaccgcgccg acgaactccg cacccgcctt ggcatcgcgt accgcatcac cggactcgcc
     2281 tcaagaaccc taggctggat cgcggattcg caaggcgtga acgtagacga agtatgtggc
     2341 gcgggcgccc ccgcccgcga aaagccggca gcggatccaa atcgcagcgc cagccaactc
     2401 gcagcgagca ccgtccggga ctggctcgcc gccgcccaag ccgacgtcct ctttgaagcc
     2461 acctctctca acgtgaaaga cggcgagccc gccgtcgccc a
//
LOCUS       DATMGX010000821         2414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_463825, whole genome shotgun sequence.
ACCESSION   DATMGX010000821 DATMGX010000000
VERSION     DATMGX010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463825"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VNR65_13470"
     CDS             complement(<1..1208)
                     /locus_tag="VNR65_13470"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWJ19728.1"
                     /translation="MRFTDVFIRRPVLAFVISALILLLGLRSLTTLPVRQFPLLTNTV
                     VTVTTVYPGATAELMQGFITTPLQQAIATADGIDYITSSSVLGASTITAYIRLNFDPD
                     AAVTEVMSKVQQVRYLIPPGANDPVINKSTGQTTAIMYIGFSSPSLALPAISDYLNRV
                     VQPILATVEGVASADVIGGQTFAMRIWLDPVRMAARGLSAAEISAAIRANNYQTAPGQ
                     QKGYFTIANITANTDLQSVDAFREMVVKVSEGGLIRLRDIATVSLDAQSFDASVSMDG
                     QNAVFIGVQPTPTGNPLTVVTGVRKKLESIRNTLPPTLMMNVAYDASKFIQASIDDVI
                     WTLGLAVAVVVVVIFLFLGSIRTVLIPVVTIPLSLIGAGMLMLALGFSINLLTLLAMV
                     LAIGLVVDDA"
     gene            complement(1226..>2414)
                     /locus_tag="VNR65_13475"
     CDS             complement(1226..>2414)
                     /locus_tag="VNR65_13475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWJ19729.1"
                     /translation="KTRIGPCSGLMSPFPQTSLGVEAVRWRVLLALLFVGVVGYALYF
                     FVGFKNAAMKSYFAAHRPPPAPVEVAVAKRADVPQSLAAIGTLTADQQVNVTTQIAGQ
                     VRNIYFESGQRVKKGDKLVQLDDAPEHGDLANFAAQAQLAEANLSRTMTLLKSDFAAR
                     QTVDQNRSIMDQAKAQMQKTQAIIDQKLIIAPFDGVLGIRQINLGQYIGPGTNIVSLT
                     NLDILHVDFRLPEQAASKLTLNQDVDLAVDPVPGKRFKAKITALEPRVNEASRMLSIQ
                     ATLANTGEILRPGMFAEVSVVLPPKEDVVTVPVTALDATLYGASVLVLEPAGNPGEKI
                     FKLKRETVTPGLYFGNQVEIAGGLKGGETVVASGQTKLQTGALVTPVDATALIPPAKV
                     ALP"
BASE COUNT          510 a          670 c          734 g          500 t
ORIGIN      
        1 atggcatcat caacgaccaa gccgatggca agaaccatag caagcagcgt gagaaggttg
       61 atgctgaagc ctaacgccag catgagcatc ccggcgccaa tgagcgacaa aggtatcgtc
      121 acaaccggga tcaggaccgt acggatcgac ccaaggaaca ggaatatcac gacaacgacg
      181 accgccaccg ctaggccaag cgtccaaatc acgtcatcga tcgaggcttg gatgaacttg
      241 ctggcgtcgt aagccacgtt catcatgagc gtcggcggga gcgtgttcct gatgctttcg
      301 agttttttgc gaacgccggt cacgacggtc aggggattgc cggtcggcgt gggctgaacg
      361 ccaataaaga ccgcgttctg gccgtccatc gaaacgctgg cgtcgaaact ttgcgcatcg
      421 agcgacaccg tggcgatatc gcggagcctg atgagcccgc cctcactcac cttcacgacc
      481 atctcgcgga aagcgtcgac gctttgcagg tccgtgttgg cggtgatgtt ggcgatggtg
      541 aagtagccct tctgctggcc gggcgcggtc tggtagttgt tggcgcggat tgcggccgag
      601 atttcagcag cgctcagacc gcgcgcggcc atgcgaaccg ggtccagcca gatacgcatt
      661 gcgaaggttt gcccgcctat cacatcggcc gaggcgacgc cctccaccgt ggctaggatg
      721 ggctgcacaa cgcggttcag ataatcggag atggcgggca gcgcgagcga ggggctcgaa
      781 aacccgatat acatgatcgc ggtcgtctga cccgtactct tgttgatcac aggatcgttg
      841 gcgcccggcg ggataaggta gcggacctgc tgcaccttcg acatgacctc ggtgacagcg
      901 gcatcgggat caaaattcag tctgatatag gccgttatcg tgctcgcgcc caagaccgag
      961 gaggacgtga tgtaatcgat cccatcagcc gtcgcgattg cttgctgcag gggcgtcgta
     1021 atgaagccct gcatcagttc ggctgtggct ccgggataga ccgtcgtcac cgtgacgacc
     1081 gtattggtga gaagcgggaa ttgccgaacg ggcagcgtcg tgagcgagcg caacccaagg
     1141 agaaggatca gggcgctgat gacaaaagcc agaacaggcc ttcggataaa gacatcggtg
     1201 aagcgcatcg tgtcgggcct gactgttatg gaagggcaac ctttgcaggc ggaatcagcg
     1261 ctgtcgcatc gaccggagtt acaagggcgc ctgtctgcag tttcgtttgc ccggacgcga
     1321 ccaccgtctc accgcctttc aaacctccgg cgatctcaac ctgattgcca aagtaaagac
     1381 cgggtgtgac tgtctcacgt ttcagtttga agattttttc gcccggattt cctgcaggct
     1441 cgagcactaa gacactcgcc ccataaaggg tcgcatcaag cgccgttacg ggaactgtca
     1501 cgacatcttc ctttggcggc aagacaaccg aaacctccgc gaacatgccg ggcctgagta
     1561 tttctcccgt gtttgcgagc gtggcctgga tgctgagcat gcgggacgct tcgttcacac
     1621 gcggttcgag ggctgtgatc ttggccttga atcgtttgcc ggggacggga tcgacggcaa
     1681 gatcgacatc ctgattgagc gtcagcttcg aagcagcttg ctcaggaagt ctgaagtcga
     1741 cgtgaagaat gtcgagattg gtcaggctga cgatgttcgt tcctggaccg atatattgtc
     1801 ccagattgat ttggcggatg ccgagcacac catcgaatgg ggcgatgatg agcttctggt
     1861 ctatgatcgc ctgcgtcttt tgcatctgcg ctttggcttg atccatgatg gatcgatttt
     1921 gatcgactgt ctgacgcgcc gcgaaatcgc tcttaaggag cgtcatggtc cggctcagat
     1981 tggcttccgc aagctgagcc tgcgctgcga aattcgccag gtccccgtgt tccggcgcgt
     2041 catcgagctg cacgagtttg tcgccctttt tcacgcgctg cccggattca aaatagatat
     2101 ttctcacctg gccggcgatc tgcgtggtca cgttgacctg ctggtcggcc gtaagtgtgc
     2161 cgatggcagc gagagattgg ggcacgtccg cgcgtttggc gacggcaact tccacagggg
     2221 ccggtggcgg ccggtgggct gcaaaataac ttttcatcgc ggcgttcttg aagcccacga
     2281 agaaatacag ggcatatccg acgacgccga cgaacagcaa agcaagcaag actctccaac
     2341 gcactgcttc caccccaagc gatgtttgcg gaaagggcga cattagcccc gagcaaggtc
     2401 cgatgcgagt ctta
//
LOCUS       DATMGX010000822         3381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_336008, whole genome shotgun sequence.
ACCESSION   DATMGX010000822 DATMGX010000000
VERSION     DATMGX010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336008"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3352)
                     /locus_tag="VNR65_13480"
     CDS             complement(<1..3352)
                     /locus_tag="VNR65_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ19730.1"
                     /translation="MLAERSADIARLGRSLPGADAHLLQPLDEQKLLGRLAEMVVSPG
                     EDHAAPEPAALRIEGCRINLPGRTFVHADGREVALTRAESALLIAFVRNPGRVLSRDQ
                     LSQAIAGHGEELYGRSIDMHVGRLRRKIEPDPKAPRFIITVSGAGYKFAAGRRTVEED
                     RESPAAIEPREQMEAAPAQARSPRINLPHSGSERRQLTVLSCELMGSMPAAIDADPED
                     LAGVVHHFQEACAGAITDMGGSIAAFTGHEVLALFGYPQAYEDDAERAVHAGLDLAAR
                     MEELVWPSGRPLQVRIGIATGLVVVADQAVVGEPSTVAPRLRSLAPAGSILIAASTRK
                     LLGGAFICDDASSYELAGISEKVTACLVAGRRSIENRFSSTRGPRLTQFVGRQHELQQ
                     LLTLWDRAKANQGQVALLCGEAGIGKSRVCEVFLERINAKPHVTIRYQCAPHHANSPF
                     YPIIGQIEHAAHIKRGDTPNAKLEKLRVALSESGAPTPADVRSLAALLSIQTGEVPAQ
                     GVTPQRRKDLTIAALIRHTLGLARDQPLVIELADAHWADSSTLELFSRIIASIKAAQV
                     FVLISFRPEFFPQWLNEPHVTMLRLDRLGREQIEAMIFDVAGQRALPSEMYAQIISKT
                     DGVPLFVEELTKSVLESGLLEDAGAQLTSVRPLPLLAIPMTLLGSLTARLDRLGPVKE
                     IAQVGAAIGREFSYRLLAAVAPISGPPLHAAIAQLAAPELIFVRGEPPDSTYVFKHAL
                     VQDAAYGTLVRSKRQQLHRRIADALEQGFPETVETQPELLAHHLIQAGLTERAIDYLR
                     KAGQRTIERSANAEAIRHLTQALELLQSRPEGPARRRAALGLEVMLSQAMIAVYGYAA
                     RKTAEVLLRAKDLIDEVTDPAQKLAILYGIWAGLYVGGEVAKQTDAAGEFLKEAERNN
                     DMAALSVAHRIMGTTYATKGEFAAALPHLEQARALGDSQHGAQLQYQYGQDVGAAALC
                     YLSWTLWHLGSVDQASQVAVDAVKRAEELSHPHTQAFTICHARGMIDIFRRSSEDMRS
                     YARSVISLCQEHGLSHWMACGRILEGWATVNEGEIDRGTELLRAGVAAWREAGARLWL
                     PLFLVLEAQACNEAGRNEAALGAIEQAIA"
BASE COUNT          653 a         1051 c         1029 g          648 t
ORIGIN      
        1 tagcgattgc ctgctcaatc gcacccagcg cggcctcgtt gcggcctgct tcgttgcaag
       61 cctgggcctc gagcaccaga aagaggggca gccacagccg cgctcctgcc tctcgccagg
      121 cggccacacc cgcgcgaagc agctcggtcc cgcgatctat ctcgccctca tttacggttg
      181 cccatccctc gagaatgcgc ccgcaggcca tccagtgtga cagaccgtgt tcctggcaga
      241 gtgagatgac tgagcgcgcg taggatctca tgtcctccga gcttcgccgg aaaatatcga
      301 tcatgccgcg ggcatggcaa atggtgaagg cctgggtgtg cggatgggac agctcctccg
      361 cgcgtttcac tgcatcgacg gcaacctgcg atgcctggtc gacggagccc agatgccaca
      421 acgtccagct caggtagcac agggcagccg cgccgacatc ctggccatac tggtattgca
      481 gctgagctcc gtgttgggag tcgccgagcg ctcgggcttg ctcgagatga ggcagtgcgg
      541 cggcaaattc cccttttgtt gcgtacgtag tgcccattat gcgatgagcg acagacagag
      601 ccgccatgtc attgttacgt tcggcctctt taaggaactc gccggctgcg tccgtctgct
      661 tggcgacctc accgccgaca taaaggcccg cccaaatccc gtaaaggatg gccagcttct
      721 gtgcgggatc ggttacctcg tcgatgaggt ccttcgcccg gagcaacact tcggcggtct
      781 tgcgtgcggc gtagccgtag acggcaatca ttgcctggct gagcattacc tccaacccga
      841 gcgccgcacg tctgcgtgcc ggaccctcgg gacgagattg cagcaattcg agtgcctggg
      901 tcaggtgacg gatcgcttcg gcgttcgccg aacgctcgat cgtacgctgg ccggcttttc
      961 gcaagtaatc gatggcccgc tcggtgaggc cagcctggat gagatgatgt gccaggagtt
     1021 cgggctgggt ctcgaccgtc tcggggaaac cctgctcgag agcatcggca attcggcgat
     1081 gaagttgctg gcgcttgctg cgcaccaggg tgccatatgc cgcgtcctga accagcgcgt
     1141 gcttgaatac ataggttgaa tccggcggtt cgcctcggac aaagatcaac tcgggagccg
     1201 cgagttgggc aatagccgcg tgcaacggtg gccccgatat aggtgcgaca gcggcgagca
     1261 gccgatagga gaattcgcga ccgatggccg cgccgacctg agcgatctcc ttaaccggcc
     1321 caagacgatc caggcgggcc gtcagcgagc caagcagcgt catgggaatc gcaagcagcg
     1381 gaagtgggcg gacggaggta agctgagcgc ctgcatcttc aagcaggccc gattccagca
     1441 ccgacttcgt gagttcctcg acgaacagcg gaaccccatc cgtcttgctg atgatctgcg
     1501 cgtacatttc cgagggcagg gccctttgtc cggctacgtc gaatatcatg gcctcgatct
     1561 gctcgcgccc tagtcggtca agccgcagca tggttacgtg cggctcattg agccattgcg
     1621 ggaagaactc cggcctgaag cttatcagga cgaatacttg ggccgctttg attgaagcga
     1681 tgatacggct gaacagttcg agggtgctgg aatcagccca gtgtgcgtcg gcaagctcga
     1741 taaccagcgg ctggtcgcgt gcaagaccca atgtgtgccg gatcagcgcc gcaatcgtga
     1801 ggtccttccg ccgctgaggt gtcacgcctt gcgccgggac ttcacctgtt tggatcgaga
     1861 gcagggcagc aagggaacgc acgtcggcag gggttggggc tccggattcg gacagggcaa
     1921 ctctcagctt ctcgagcttg gcattagggg tgtcccctcg cttgatatga gccgcatgct
     1981 cgatctgccc gataatcgga taaaacgggc tgttcgcatg gtgcggagcg cactgataac
     2041 ggattgtgac atgcggcttt gcgttgatcc gctccagaaa gacttcgcaa actcgcgact
     2101 ttccgatgcc ggcttcaccg cacagcagcg cgacctgacc ttggttggct ttggctcgat
     2161 cccataaggt caatagctgc tgcaattcgt gctgccggcc gacaaactgt gtaagtcgcg
     2221 gcccacgagt agagctgaac cggttctcga tggaccgcct tcccgcgacc aggcaggcgg
     2281 tcaccttctc ggaaattccc gcaagctcgt acgagctggc atcatcgcaa atgaaagcgc
     2341 caccaagcag cttgcgcgtg ctcgcggcaa taaggatcga acctgcggga gctaagcttc
     2401 gcaaacgagg agcaacggtt gaaggttctc cgaccacggc ttggtctgcg accacgacca
     2461 ggcccgtagc gattccgatt cgtacctgca acggtctgcc ggagggccat acgagctcct
     2521 ccatccttgc cgcaagatca agccccgcgt gcacggcacg ttccgcatca tcctcgtaag
     2581 cctggggata accgaataac gccaaaacct cgtgccctgt gaaagcagcg atgcttcccc
     2641 ccatatcggt aatcgcgcct gcgcaagcct cctggaaatg atggacgaca ccagcgaggt
     2701 cctcggggtc ggcatcaatg gcggccggca tcgaacccat aagctcacag gacagcacag
     2761 tcagctgccg tctctcggac ccggaatgcg gcaggttgat gcggggactg cgtgcttgcg
     2821 ccggcgcagc ctccatttgc tctcgcggtt cgatggccgc cggtgactcg cgatcttctt
     2881 cgacagttcg gcggccggcg gcaaatttgt agccagcacc tgacaccgtg atgatgaacc
     2941 gcggcgcttt tgggtccggc tcgatctttc gccgcagccg gccgacatgc atatcgatgc
     3001 tgcggccata cagctcctcg ccatgcccgg ctatagcctg actcaactga tcgcgcgaca
     3061 acacgcggcc cggatttcgc acgaatgcga tgagcagcgc agactccgct cgggtcagcg
     3121 ccacctcgcg tccgtccgca tgcacgaaag tccgaccggg caggttgatc ctgcagccct
     3181 cgatgcgcag agccgccggt tctggtgcgg cgtggtcttc cccaggggaa actaccatct
     3241 cggcgaggcg accgagaagc ttctgttcat ccaatggttg caacagatgg gcgtccgcgc
     3301 caggaagaga acgacccagc ctggcaatat ccgctgatcg ctcagccagc acgatcaatc
     3361 tcggaactcg gtcgctcagt c
//
LOCUS       DATMGX010000823         2652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_393098, whole genome shotgun sequence.
ACCESSION   DATMGX010000823 DATMGX010000000
VERSION     DATMGX010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393098"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1043)
                     /locus_tag="VNR65_13485"
     CDS             complement(24..1043)
                     /locus_tag="VNR65_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19731.1"
                     /translation="MRSSFAIVVTLLLALTAPAQMTPAQNQAPSPNAQPQTLGGAPNQ
                     SPSGAPSASSNNPQAPSSKAGGHKLPQAKTQDEFKAYQDAASKTDPAQMEAAANAFAA
                     KYPNSELKAALYVRAMNMYGQANNSEKVIETGRKAIAADPANPVPLVQVGSALVEGTR
                     DTDMDRDQRLAEAAKDAQGAIDNIGGLLVPPNAPPDRVAAVKASILTMAYDSLGMVNL
                     NKKDYAAAEQELLKAVDASKAQPEAVVFLRLSVAQDQLKQYPQALESATKAAQYAPQG
                     SAAQNLAKQQQARLQKLIAGGTPAVPSGTAPATPAPAAPATQAPAQTPPAQTSPAQTP
                     PNPPH"
     gene            complement(1098..2579)
                     /locus_tag="VNR65_13490"
     CDS             complement(1098..2579)
                     /locus_tag="VNR65_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19732.1"
                     /translation="MLPLVLASFACAARAQSKATLDVSETLFSVVAAMNVCGYDQELQ
                     SSSPIRMEVRADLVEASKSPAAAAAAKEMCNFYREHRQSDGAHDLAQYVSLALYLGPP
                     PDFTPKVKEADMPPDSTYVLGFVPLLKQYYAAANLHSIWLKHQSEYLALIDRYHEPVA
                     HMISSTDTYLRMPLAGYLGRSFTVYLEPMSAPGQVNSRNYLQDYYYLVISPSGDNIHM
                     DALRHTYLHFVLDPLIAKRATALQRLKPVLVAVQKAPMAEDYKLDPGLLVVECLIRAI
                     EARNPSAPKLPEKERQAMLQRDEAEGFIFTRFFYDELRDFEKENTGLQDAFPNWLHNI
                     DVDGEKKRASETAFASQAAPEVMRAAKPASQQKVDLAERELVSGDPAGAQKLAEEALD
                     EKEDPARAYFVLARAATMSGNMQGAQDNFQKALNATKDPRTAAWCHIYLGRILDLQDE
                     RENAIAQYRAALGAGDASPDTMKAAERGIEEPYQPPNVKREQQ"
BASE COUNT          442 a          798 c          821 g          591 t
ORIGIN      
        1 aagccatcct tgtcttttgc gcctcaatgc ggcgggttcg gcggtgtctg ggccggcgaa
       61 gtctgagccg ggggtgtctg agcgggcgct tgcgttgccg gcgcggctgg agcaggcgta
      121 gccggcgcag tgccgcttgg aaccgctggc gtaccgcccg caatcagctt ctgcaatcgg
      181 gcttgttgct gcttggccag gttctgggcc gcactgcctt gcggcgcata ttgcgcggct
      241 ttggtggcgg actcgagtgc ctgcggatat tgctttagct ggtcctgcgc taccgacagg
      301 cgcaagaata cgaccgcttc cggttgcgcc ttgctggcat ccactgcttt caggagctcc
      361 tgctcggcag cggcatagtc tttcttgttc aggttaacca tgcccagcga gtcgtaggcc
      421 atggttagaa tgcttgcctt cacggccgcg acccgatcag gcggcgcgtt tggcggcacc
      481 aggagtccgc caatgttgtc gatcgcgccc tgggcatcct tggcagcttc ggcgagacgc
      541 tggtcgcggt ccatatcggt gtcgcgagtg ccctccacta acgcggagcc gacctgcacc
      601 aggggaaccg ggttggccgg atccgccgct atggctttgc gcccggtttc gatcaccttc
      661 tccgaattgt tggcctgacc gtacatgttc atggcgcgga cgtagagagc cgccttcagt
      721 tcgctgttcg ggtacttcgc agcgaaagcg tttgccgcag cttccatttg ggcagggtca
      781 gtcttgcttg ccgcatcctg gtaggcctta aattcgtcct gcgtcttcgc ctggggtagc
      841 ttgtgtccgc cagccttgga agacggcgcc tgcgggttgt tggaggaagc gcttggcgca
      901 ccgctcggcg attggttcgg agcgccgccc agcgtctgcg gctgtgcgtt aggactgggg
      961 gcctgattct gggccggcgt catctgggcc ggcgccgtca gggccagcag cagcgtcacc
     1021 actattgcga aactcgatct cattactacg tgtcctttct ggggggaaga aacgctggcc
     1081 ttcccgtcaa tgcgaagcta ttgctgctcc cgctttacgt ttgggggctg gtagggttct
     1141 tcgatcccgc gctcggccgc cttcatggta tccggcgacg catccccggc gccgagtgcc
     1201 gcccgatatt gcgcgatcgc gttttcccgc tcatcctgca agtccagaat gcgccccaaa
     1261 tagatgtggc accaggctgc ggtgcgaggg tccttggtcg cattcagcgc cttttgaaag
     1321 ttgtcctgcg ccccctgcat gttgccgctc atggtggcgg cccgagccag cacgaaataa
     1381 gctcgcgccg gatcttcctt ctcatcgagg gcctcttccg ccagcttctg cgctcccgcg
     1441 ggatcgccgc tcaccagctc tcgttcggcc aaatccacct tctgttgaga tgcaggtttc
     1501 gccgctcgca tcacctcggg cgcagcttgc gatgcaaacg cggtctcgct cgcgcgcttc
     1561 ttctcgccat cgacatcaat attgtgcagc cagtttggga aggcgtcctg caggccagtg
     1621 ttttccttct cgaagtccct caattcgtca tagaagaacc gggtgaagat gaagccttcg
     1681 gcttcgtccc gctgtaacat ggcctggcgt tccttctctg gcaatttggg agcggaggga
     1741 ttgcgcgctt caatcgcccg aatcaagcac tcgacgacta gcagccctgg atccagcttg
     1801 tagtcctccg ccatgggcgc tttctgtact gccaccagaa ctggtttcag gcgctgcagc
     1861 gccgtcgccc gtttggcgat cagcggatcg agcacgaaat gcagataggt gtgccgcagg
     1921 gcgtccatgt ggatgttatc gccgctggga gagatcacca ggtagtaata atcctgaagg
     1981 taattgcgcg agtttacctg accgggcgcg ctcatcggtt ccagatacac ggtgaagcta
     2041 cggcccagat atccggccag tggcatccgc aggtaagtgt cggttgaact gatcatgtgc
     2101 gcgaccggct cgtggtagcg gtcgatcagg gcaagatatt cggattggtg cttgagccag
     2161 atggaatgca ggttggcggc ggcgtaatac tgcttgagca agggtacgaa tcccaataca
     2221 tacgtcgaat ccgggggcat gtcggcttct ttaactttgg gagtgaaatc tggcggcggc
     2281 cccaggtaca aggccagcga aacgtattgc gccaggtcgt gcgctccatc gctctgccgg
     2341 tgctcgcgat agaaattgca catctccttg gcggctgccg cagcggcagg cgatttgctc
     2401 gcctcaacga ggtccgcccg aacttccatg cggatgggcg atgacgactg cagttcctga
     2461 tcgtatccgc agacgttcat ggcggcgacc acgctgaaca acgtctcact tacgtcaagc
     2521 gtggccttgg actgcgccct agcggcgcag gcaaacgacg ccagcacgag cggcagcaca
     2581 aacagacggc gaaaacagcc cagaagagcc tcctgacaga atttcggcgg gaatcacttt
     2641 ctaccagtgc ct
//
LOCUS       DATMGX010000824         2584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_447707, whole genome shotgun sequence.
ACCESSION   DATMGX010000824 DATMGX010000000
VERSION     DATMGX010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447707"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1619
                     /locus_tag="VNR65_13495"
     CDS             192..1619
                     /locus_tag="VNR65_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWJ19733.1"
                     /translation="MVIERASELVQFLLIVIYVLLACAVTVHVLLTKSDVRAALGWIA
                     VAWLSPVFGALFYFVFGINRVTRRALHFAKLEHERGSPAGPDARPPVPPNIAALSAIA
                     QRVTESPLIAGNAVSVLRGGDEAYPSMLAAIRNARYSVALGSYIFRDDSVGRSFIGAL
                     IDAHKRGVEVRVLVDGIGSGYILSSPLRDLEAAGIPTARFLHTWVPWRMPFLNMRNHK
                     KLLIVDGTVGFTGGLNIGADHSRWRAAKDYVDGVQVRVEGPVTRQLMDTFARDWSFTT
                     DEVLDKDAWWPPIDAAGSVSARGIHSGPDADMYKLETILGAALAQAQNRVRIVTPYFL
                     PDRRLQFAIAQAGLRGVSVEILIPQRCDYFFMDWAMLAHLRFFKDVLATVYFTPSPFN
                     HAKLMTVDGQWCLIGSSNWDTRSLRLNFEFDLECYDSAVTANVDALIEQKISQGRKVS
                     PDELASRPRWTQLRDAATRLLLPYL"
     gene            2171..2383
                     /locus_tag="VNR65_13500"
     CDS             2171..2383
                     /locus_tag="VNR65_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19734.1"
                     /translation="MSPDQDGKKSASTDPEAPAEQDTAQDQDGGLEARKPHLTGLIGM
                     HIDQPQEDTETGSPGADIDRDLADGK"
BASE COUNT          546 a          744 c          735 g          559 t
ORIGIN      
        1 caataagact atagtcgttc aaaaaacgga gtctgtgcaa tatttagtga aacggacgag
       61 cgattcaagg tcgccataga cgaaagaatc caaccgcata cgcgacgccg gccaaggtcg
      121 gcgctagaat attatgcaca catctctagc gatggttagg caaccggccc accgagctgg
      181 gagactgccg agtggtcatt gaacgggcat ccgaattggt gcagtttctg cttatagtga
      241 tctatgttct cctcgcgtgc gccgtcacgg tccacgtgtt gctgacgaaa agcgacgtcc
      301 gcgctgcgct gggatggatc gcggtcgcct ggctgtcccc agttttcggt gcgctattct
      361 atttcgtctt cggcatcaat cgcgtgacgc gccgggcctt gcattttgcg aagcttgagc
      421 acgagcgtgg aagccctgcc ggccccgatg cgcggccgcc ggtgccgccg aacatcgccg
      481 ccctttccgc catcgcccag cgcgtgaccg aaagtccgct gatagccggc aacgccgttt
      541 cggtcttacg gggaggcgat gaagcgtatc cgtcgatgct ggccgccatc cgcaatgccc
      601 gctacagcgt cgcgctcggc tcttacatct tccgcgatga ctcggtcgga cgctccttca
      661 tcggtgcact gatcgacgca cacaagcgcg gcgttgaggt gcgtgtgcta gtcgacggca
      721 tcggcagcgg ctacattcta tcgtcaccgt tgcgcgatct ggaagccgcc gggatcccca
      781 ctgctcgttt cctccatact tgggtgcctt ggcgcatgcc tttcctcaat atgcgcaacc
      841 acaagaagct cctgatcgta gatgggacgg tgggattcac tggcggattg aacatcggag
      901 cggaccactc acgctggcgc gcagcgaaag actatgtcga cggtgtgcag gtccgggtgg
      961 aaggtccggt cactcggcag ctcatggaca cctttgcgcg agactggtcg tttacgacgg
     1021 atgaggtgct ggacaaggat gcctggtggc ctcctatcga cgctgcagga tcggtctccg
     1081 cccgcggcat ccattcggga ccggatgcgg atatgtacaa gctggagacg atcctcggtg
     1141 cggcccttgc ccaggcacaa aatcgggttc gtatcgtcac accctatttt cttcccgacc
     1201 ggcgtctcca gttcgcaatc gcacaggcag gtctgcgcgg ggtttccgtc gagattctga
     1261 ttccgcagcg atgcgactac ttcttcatgg attgggcgat gctggcgcat ctgcgctttt
     1321 tcaaggatgt gctcgccact gtctatttca caccatcgcc cttcaaccat gccaagctca
     1381 tgacggtgga cgggcaatgg tgcctgatcg gaagctcaaa ctgggacacg cggagcttac
     1441 ggctcaattt cgagttcgac ctggaatgtt acgatagcgc ggtgaccgcg aatgtcgatg
     1501 ctctgatcga gcagaaaatt tcacaaggcc gcaaggtcag cccggacgaa ctcgcctccc
     1561 ggccgcgatg gacccagttg cgcgacgcgg ccacgcggct tctgctgccg tatctttgac
     1621 cgggcgtaat aaaagtcacg ccgctttcta actattttct tcttagccat ttcggctcct
     1681 ctttcgaaag agccggacgt tttatctccc gtttccgatg ttgcagccga cctgctggcg
     1741 gaacagcgta ccgtgggaga cgacgagaac acgggacgaa gccgtgagcc agcagacatt
     1801 agggcgcggc agacagaaag tgtattccca caaccataac accaagtata acgagcagga
     1861 tcagaacagc cattattatg acgcgtgaga actgccggcg gtttaagcgg tcgctcactg
     1921 aatgctcctg gatccagtct cggaaacgta acctatccgc agttcaactt cagagctctg
     1981 cggtagatca agcgctcgga acatcaatat caatgtaact ctcgtcctca gctgccgcgt
     2041 cataactatt caataacaaa gaacatctgg caacaacggc cttgatttcg atcaaggccg
     2101 gtatgagcaa actggactta gcttacctct ccggcgagta ggtcaatgtt cgagagcaaa
     2161 ggaagatgga atgtcgcccg atcaggacgg aaagaagtcc gcaagcaccg atcccgaggc
     2221 gccggctgag caagacacgg cgcaggatca ggacggcggc ctcgaagcgc gcaagccgca
     2281 tctgacaggg ttgattggaa tgcacatcga tcagcctcaa gaggatacag aaaccggatc
     2341 gccaggtgcc gatatcgatc gcgatctagc cgacgggaag taacgtcggc catcgttatt
     2401 gcgcaactta acgttcttca gcggctgtct cgcgcacccg gccattgcgg ttagtctagt
     2461 tctcggactt cctcgtcatc aaggacttgt gtgtcatctg gttgtcgagc gcctgcagct
     2521 gcgcaatcat gtcgtcggcg tttaaaatta tagcccttgc gggcattggt gttcgcgttc
     2581 tcga
//
LOCUS       DATMGX010000825         2469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_263030, whole genome shotgun sequence.
ACCESSION   DATMGX010000825 DATMGX010000000
VERSION     DATMGX010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263030"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1390
                     /locus_tag="VNR65_13505"
     CDS             212..1390
                     /locus_tag="VNR65_13505"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HWJ19735.1"
                     /translation="MLLAAVSAVSLVLAGLAGNPGFAQAHTATPAKGFRPPHTLPLTT
                     FYDAPHPLPPGEPGELIRSEPINQYNLPFELSALRILYHSRTANREDVAVSAVVLIPD
                     GKPPASGWPVIAWAHEFRGAARQCAPSLMKNLGAGPLLAMFANLGYAVVATDYSGWGA
                     DSGKPVLDMQSNALDVIYSVAAARAAGKQIGAKWIALGSFQGGLAAVALAESEVRDSN
                     YLGSVATSGLADAQETYEHYAQGSSNRMLLVLASTVKALYPEFKVSDMLNDKALPAYQ
                     RVAETCGGETEQEFTKEMLKPGWENNKLVKELFSSNTPGQKRAHSPLLVISGEADLAI
                     PAAMSGRTVARMCKQGDRILFLKYPDLDAGGVMGASVADQISWIKARFAGYAAPSNCP
                     "
     gene            complement(1439..1729)
                     /locus_tag="VNR65_13510"
     CDS             complement(1439..1729)
                     /locus_tag="VNR65_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19736.1"
                     /translation="MLRKKFKIESPFLLIALALVGLAMLAAPPPAFSQSCSLCYTQAA
                     SAGARMIQALRSGILVLIVPPTAMSVGMIFIVYRKRNQFRQDDVAPDSDPKW"
     gene            complement(1783..2103)
                     /locus_tag="VNR65_13515"
     CDS             complement(1783..2103)
                     /locus_tag="VNR65_13515"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWJ19737.1"
                     /translation="MTTAEEHKSNGAGKDLVIYVCLLALAGLQFLIAYQNIDISQMFT
                     RMLAVACIEAGLAILFFMHLWTEKRAFLIFVVVFTISVLLGMQYGWTDSTRMLVGAPF
                     SRMM"
     gene            complement(2129..>2469)
                     /locus_tag="VNR65_13520"
     CDS             complement(2129..>2469)
                     /locus_tag="VNR65_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWJ19738.1"
                     /translation="AGAFRWTMITAALGIVFSLLHIREWMGLIAEGMTLFKNPWGTGL
                     FGASFFSITGLHLTHVVSGVIALLVVGVRYKGGRYNADDIEILGLYWHFVDLVWMFVV
                     PLVYLLNLTH"
BASE COUNT          581 a          658 c          723 g          507 t
ORIGIN      
        1 cgcggaggag aagattaaac gttgggcaat tcctcttttt gcataaaacc agctctgggc
       61 aaggacttaa gggcggtcgg actgagataa ggaccaggat ttcgcccgta cgcgggcggt
      121 tgtggcctct acgaatctta aggtgatacc atcccctact gacaccatga caaaggggcg
      181 cttgttccgg ttatcaaatt gccatctaca aatgcttctg gcggcggtca gtgccgtttc
      241 tttggttttg gcagggttgg ctggaaatcc aggatttgcc caagctcata ccgcaacacc
      301 ggccaaagga ttccggcccc cccacactct tccgctgacg actttttatg acgcgccaca
      361 tccgctgccg ccgggcgaac ctggagaact gatacgctcg gaaccgatca accaatacaa
      421 ccttccattt gaattgtcgg cattgcggat tctttatcac tcccgtaccg caaataggga
      481 agacgtggcc gtctcggcgg tggtgttgat tccggatggc aagcctccgg caagcggatg
      541 gccggtaatc gcgtgggcgc acgagttcag gggagcggcg cgacagtgcg ctccgtcact
      601 gatgaagaat ctcggcgcag ggccgctgct tgctatgttt gccaatctgg gttatgcggt
      661 ggttgcgacc gactattccg gttggggggc cgattccggc aagccggtgc tggacatgca
      721 gtcgaacgcg ctagacgtca tctattcggt tgcagcggcg cgcgcggctg gcaaacagat
      781 tggagccaaa tggatcgctt taggatcttt ccagggaggt ctggcggccg tggcactggc
      841 ggagagcgaa gtacgcgatt ccaactatct tggcagcgtc gccacttcgg ggctggctga
      901 tgcgcaggag acctacgagc actacgcgca gggctcttcc aatcggatgc tgctagtgct
      961 ggcatccacc gtgaaagcgc tttatccgga gttcaaagtg agcgacatgc tgaacgacaa
     1021 ggcgctgcct gcgtaccagc gtgtggccga gacctgcggc ggagaaacgg agcaggagtt
     1081 cacgaaggaa atgctcaagc cgggttggga gaataataaa ttggtaaagg agctcttcag
     1141 cagtaacact cccggtcaaa aacgggcgca cagcccgttg ctcgtgatca gcggcgaggc
     1201 ggaccttgcc ataccggcgg cgatgtcggg aaggaccgtg gcgcggatgt gcaagcaggg
     1261 agaccgaatt cttttcttga agtatccgga tctcgacgcc ggcggcgtga tgggagcttc
     1321 ggtggcagac cagatcagtt ggatcaaggc gcggtttgcc ggctatgccg ccccgagcaa
     1381 ctgcccttga ctccggagac ggggcaagcc cgactctaca ggtggaagat ctacgcggtt
     1441 accacttagg gtcagagtca ggcgcgacat cgtcctgcct gaactgattg cgtttgcgat
     1501 agacgatgaa aatcatgccc accgacatgg ccgtcggagg gacgataaga accagaattc
     1561 cgctgcgcaa agcctggatc atgcgagcgc cggcggaggc cgcctgcgtg tagcagaggg
     1621 agcaactctg ggagaaggcc gggggagggg cggcgagcat ggccagtcca accagcgcaa
     1681 gagcaatcag caggaatgga ctctcgatct tgaacttttt tcttaacatc ttttctctga
     1741 actcttctcc gaactgggca ctcaactggc gccttttgct tcttacatca ttcttgaaaa
     1801 tggagcgccg accagcatgc gagtgctatc ggtccatcca tattgcatcc ccagcagaac
     1861 tgaaatcgta aagacaacga caaaaatgag aaaggcccgc ttttctgtcc aaagatgcat
     1921 gaaaaacaat attgccaggc ccgcctcaat acaggcgacg gccagcatgc gggtgaacat
     1981 ttgcgatatg tcaatgttct ggtaggcaat gaggaactgc aagcctgcga gagcgagcag
     2041 gcaaacataa atgaccaaat cttttccggc tccgttactc ttgtgctctt cggctgttgt
     2101 catagctttc gctccctaag tttctgatct agtgggttag attcaaaagg tagaccaacg
     2161 gtacgacgaa catccaaacc agatcgacga agtgccagta aaggccgagg atctcgatat
     2221 catccgcatt gtagcggccg cccttgtagc gcacaccgac gacgagcaga gcgattacac
     2281 cgctcacaac gtgagtgaga tgcaagcctg tgatgctaaa gaatgacgcc ccaaacaatc
     2341 ccgttcccca gggattctta aaaagggtca tgccttcagc gatgaggccc atccattcgc
     2401 gaatatgcag caaagagaaa acaataccga gagccgcggt gatcatggtc cagcggaagg
     2461 ctccggctt
//
LOCUS       DATMGX010000826         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_8227,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000826 DATMGX010000000
VERSION     DATMGX010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8227"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="VNR65_13525"
     CDS             <1..1436
                     /locus_tag="VNR65_13525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ19739.1"
                     /translation="TEEPFEAEYRVRGAANSRTVLSRGHVYTGSSGQKMFSGALIDIT
                     EEKAAEQALQRELEYRRLAEAKLQAINSTLEQRVETEVAERIKAEEALRQAQKMEAIG
                     QLTGGVAHDFNNLLTVIMGGLDIIRRSKPEDHARIKRATDMALQGTQRAASLTGRLLA
                     FSRRQPLEPKPLELTALVREMTELLHRTLGERIELEAVMASRLWPVEVDLNQLESAIL
                     NLAVNARDAMPDGGKLTIEAANTALDESYSARDTEVIPGQYVMLSVSDSGSGMSKETL
                     DHAFEPFYTTKEVGRGTGLGLSMVYGFVKQSGGHVTLYSERGQGTTVKLYFPRYKGKL
                     SAAEIEERPSTPTASHEEVVLVVEDNDDVRSYSVTILRELGYKVLEAADAEKALQILE
                     GTDRVDLLFTDVVLPGKNGRVLADSAKRLRPALRVLFTTGYSRNAIVHQGRLDADVHL
                     ISKPFTFDQLAARVRDIIDLPVPKQLS"
     gene            1707..2462
                     /locus_tag="VNR65_13530"
     CDS             1707..2462
                     /locus_tag="VNR65_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19740.1"
                     /translation="MKTTLHSFAEGISKLGLTEVGHAIALLWYFDLQEGKPELRPAFL
                     AGLMHDLSLRRQVNATRLGRQLLANRAVMRGKEMGTVRLRLASRSRLSSRYNEIAGRV
                     VAPAPGAHIIPAGLFDNTRPYLQTLVVQINGTYQNGFYDACAVMCRRLLNCLVLLAFE
                     SAAKGKVIRDASGEYRAFAHIIELASSNRHLKLARGAGAVMGKMDYVGDLAAHHPTYT
                     TRQKDIDEQRLAFHRVILELADLAEIKSQATAD"
     gene            complement(2485..>2719)
                     /locus_tag="VNR65_13535"
     CDS             complement(2485..>2719)
                     /locus_tag="VNR65_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19741.1"
                     /translation="FLAASTAHRAPGGLAVGQLLEGNGSIVMKDGKPIGQDASGKAVI
                     RLASGTFAALSGKTLKWVSKPTGPNRFEQEYSE"
BASE COUNT          600 a          782 c          808 g          529 t
ORIGIN      
        1 aaaccgaaga gccgttcgaa gcggaatatc gcgttcgagg tgcggccaac tcgcgtaccg
       61 tattgtcacg cgggcacgtc tataccggat cgtccggtca aaaaatgttt tcgggcgcat
      121 tgatcgacat caccgaggag aaagcggccg aacaggcgct tcagcgcgaa ttggaatatc
      181 gacgacttgc cgaggcaaag cttcaggcaa tcaacagcac gctggagcag cgggtcgaaa
      241 ccgaggttgc ggagcgaata aaggccgagg aggctttgcg ccaggcccaa aaaatggagg
      301 cgatcgggca attgaccggc ggcgtcgccc atgacttcaa caacctgctc acggtgatca
      361 tgggcggtct cgacattatc cgcagatcga agccggaaga tcatgcccgc ataaaacggg
      421 cgaccgacat ggcacttcaa gggacccagc gtgccgcgag tctgacgggg cggctgctgg
      481 ccttttcaag gcgccaaccg ctggagccaa agccgctgga attgaccgct ctcgtgcggg
      541 agatgactga actgcttcat cgaacgctgg gtgagaggat cgaactcgaa gccgtgatgg
      601 cctccaggct ttggcccgtt gaggtcgacc taaaccagct ggagagcgcc atcctcaact
      661 tggccgtcaa cgcgagggac gcgatgcctg acggcggcaa gttgaccatc gaagcagcaa
      721 atacggcgtt ggacgaatcc tactccgcca gggatacgga ggtcattccg ggtcagtacg
      781 tgatgctatc cgtcagtgac agtggttcag gaatgtcaaa ggaaacgttg gaccatgctt
      841 ttgaaccatt ctacaccacc aaagaggtgg gacgcgggac gggccttggc ctcagcatgg
      901 tttatggatt tgtaaagcag tctggcggtc acgttacgct gtatagcgag cgaggtcagg
      961 ggacgacagt gaagctctat ttcccccggt ataagggaaa attgagcgcg gccgagatcg
     1021 aagaacggcc atcaactccc acggcgagcc acgaggaagt tgttctggtc gttgaggaca
     1081 atgacgacgt acgctcctac agcgtgacca tcctgcgaga gctcggatat aaagtacttg
     1141 aagctgcaga tgccgaaaag gcgctgcaga tcctcgaagg taccgaccgc gtcgatctgc
     1201 tgttcaccga cgtggtattg cccgggaaga atgggcgcgt attggccgat tccgctaagc
     1261 ggttgcgtcc tgccttgcgt gtcctgttca cgacaggcta ctcgcgtaac gcgattgttc
     1321 atcaaggccg cctggacgcg gatgtccacc tgatcagcaa gccattcact ttcgatcagc
     1381 tcgcagcccg ggtacgcgac atcatcgatc taccggtgcc gaagcaactc tcgtgaacgg
     1441 ccgcttcgcg cgccgcaacc ggacgttccc tcggcgcttt ttgacccggt gcggaggtcg
     1501 actgtccagg gaagcaatag ccgaggaata gaccgcgctt tccggggttc acacccagga
     1561 cttggaccgt tcaggtcgat aatgcagttc ggacatcaac ccgcgcattg caacgacgaa
     1621 gtccgcgtcg aagagaagcg actccggtcc cgcataattt gacgggtaag cccactcaga
     1681 attttagaaa cggcgctttg caggcaatga aaactaccct tcattctttc gccgagggca
     1741 tctcgaagct cggcctgacg gaggtcggcc atgccatcgc gctgctttgg tactttgatc
     1801 tgcaggaagg caaaccggag ttgcgtcctg ctttcctggc cgggttgatg catgatctct
     1861 cgcttcgccg ccaggtcaac gccacccgcc ttggaaggca gctgcttgca aaccgtgccg
     1921 tgatgagggg taaggagatg ggcaccgtga gacttcgcct tgccagcagg tcgcgcctgt
     1981 catcccgcta taacgaaatt gcgggaaggg tcgttgcacc cgcgcccggc gcacacatca
     2041 ttccagcggg actgttcgac aataccagac cgtatctgca aaccctggtg gtgcagatca
     2101 acggaaccta ccagaacggt ttctacgacg cctgtgccgt catgtgccgg cgcttgctga
     2161 attgcctggt gctccttgcg ttcgaaagtg cggcaaaggg caaagtcata agagatgcct
     2221 ccggggaata ccgggcgttc gcccacatca tcgaattggc ttcctccaac cggcacttaa
     2281 agctcgcccg gggcgcgggc gcggtgatgg gaaagatgga ttatgtgggt gatcttgccg
     2341 ctcatcaccc gacttacacc acccgacaga aggacatcga cgagcagcgg ctggcgtttc
     2401 accgcgtcat tttggaactg gccgatcttg ccgaaatcaa gtcgcaggcc acggctgact
     2461 gaaagaggcg gcctgtcttt gaggttactc cgagtattcc tgctcgaacc gattcgggcc
     2521 cgtaggctta gagacccatt tgagcgtctt tccagacagc gcggcaaatg tgcctgatgc
     2581 caacctgatc acggccttcc ccgacgcatc ctggccgatt ggcttgccgt ccttcatcac
     2641 gatcgacccg ttgccctcca acagctgccc gaccgccaac ccaccgggtg cgcggtgagc
     2701 ggtggaggca gccaaaaag
//
LOCUS       DATMGX010000827         3639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_151196, whole genome shotgun sequence.
ACCESSION   DATMGX010000827 DATMGX010000000
VERSION     DATMGX010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151196"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VNR65_13540"
     CDS             <1..459
                     /locus_tag="VNR65_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWJ19742.1"
                     /translation="GPGDPAATGDYTLPVIQKVIDSGIPLFGICLGHQLLGLALGGRT
                     IKMPHGHHGANHPVMDYATGKVEITSMNHGFAVERESLPHSVEETHVSLFDGSNCGIR
                     LKGLPVFSVQYHPEASPGPQDSHYLFIRFVNMMRERRSLASQDERSSRLS"
     gene            672..1955
                     /gene="hemA"
                     /locus_tag="VNR65_13545"
     CDS             672..1955
                     /gene="hemA"
                     /locus_tag="VNR65_13545"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HWJ19743.1"
                     /translation="MDYHGKFAQALEALTSEGRYRVFADILRHRGEFPRAVHHTPEGT
                     KPITVWCSNDYLGMGQHPSVLSAMHEAIDTVGAGSGGTRNISGTTHYHVELEQELADL
                     HGKEAALLFTSGYVSNEATLSTLVKILKGAIIFSDEKNHASMIEGVRQGGGPKRIWRH
                     NDLEHLESMLQEYGPDVPKIVAFESVYSMDGDISPISDICDLAKKYGALTYIDEVHAV
                     GMYGEHGAGVAERDNAMHKIDIIEATMAKGFGLMGGYIASSKACVDAIRSYAPGFIFT
                     TSLAPVICAGALASIRHLKTSQVEREMHQLRAAVLKERLALENLPVMHTPSHIVPVLV
                     GDPVQCKALTDSLLDRFGIYVQPINYPTVPRGTERIRLTPGPNHTDEQMDHLVSSLRV
                     MWDEMGLRSGYEWCQLWRGRIAVAGQLDLAVAAGG"
     gene            2164..2603
                     /locus_tag="VNR65_13550"
                     /pseudo
     CDS             2164..2603
                     /locus_tag="VNR65_13550"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            2940..3524
                     /locus_tag="VNR65_13555"
     CDS             2940..3524
                     /locus_tag="VNR65_13555"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWJ19744.1"
                     /translation="MTRNRLAILAVSTLLATTLDASADPSAYAFDPMHTEVRFCWNHL
                     GISRQCAHFTGYDGELLYDEANPEKSQLKVTFKTDSIETLVPIFNDHMKGDKLFDTKK
                     FPEATFQSTKFERTGEKAGRVSGDLTLHGVTKPVTLDVTLNFAGIQPMSKKPTLGIGA
                     KTNVKRTDFGVSAAAPYVSDEVSIEIQTEMFKKS"
BASE COUNT          845 a          983 c         1013 g          798 t
ORIGIN      
        1 ggaccggggg atcctgccgc gactggagac tatacattac ccgtcatcca aaaggtcatc
       61 gattctggga taccgctgtt tggcatatgt ctcggccatc agcttttggg gctcgcgctc
      121 ggcggccgaa cgataaaaat gcctcacggg catcatggag caaatcatcc cgtcatggat
      181 tatgcgaccg ggaaggtcga aattacttca atgaaccatg gttttgcagt tgaacgcgag
      241 agcctgcctc attctgtgga ggaaacgcac gtctctttat tcgatggaag caattgcggc
      301 atccggctta aggggctgcc tgtgttctcg gtccaatatc atcctgaggc gtcacccggc
      361 ccccaggaca gtcactacct attcattaga tttgtaaata tgatgagaga gaggcgatcc
      421 cttgcgtcgc aggatgagcg cagctcaagg ttgagctaat gagccggcgt cgactgtcta
      481 gttgcgttta cacctcaaaa tgtcaaaaaa agttgacgtg cgtcagtttg taatgcttct
      541 aatgcctgga cttccgttcc tgttcgcgta attatctggc gctacaggcg gaattcctgc
      601 cagtaagact taagctggct ctttcgccag gcgcatcagt gtgtgcgccg acccgccaac
      661 gaggaatgct tatggactac cacgggaagt ttgcccaagc tctggaagca ctgacttccg
      721 agggccggta tcgcgtattt gccgacattc tgaggcaccg cggagagttt ccgcgcgcgg
      781 ttcaccacac tccggaaggc acgaagccca tcaccgtatg gtgctctaac gactatcttg
      841 gcatgggaca gcatccatcc gtgttgtccg ccatgcacga ggcgatcgac actgtcggag
      901 cgggctccgg cggaacgcgc aacatttctg gaacgaccca ctatcacgtt gagctggaac
      961 aagaactggc cgatctgcac ggcaaggaag cggctcttct cttcacgtca ggctatgtct
     1021 cgaacgaggc gacgctttcg acgcttgtga agatcctgaa gggcgcgatt atcttctcgg
     1081 acgagaagaa ccatgcgtcg atgatcgaag gcgtgcggca aggcggcggc ccaaagcgca
     1141 tttggcgcca caacgacctt gagcacctgg aaagcatgct tcaggaatat ggcccggacg
     1201 ttccgaagat cgtggcgttc gaatccgttt attccatgga tggcgatatc tcgccgatca
     1261 gcgacatctg cgatctggcc aagaaatatg gtgcattgac ctatatcgac gaagttcatg
     1321 ccgtcggtat gtatggcgaa catggcgcgg gcgtagccga acgcgacaat gcgatgcata
     1381 agatcgacat catagaagcg acgatggcca agggcttcgg cttgatgggc ggttacatcg
     1441 cttcgagcaa ggcctgcgtt gacgcaattc gctcttacgc acccggtttc atcttcacga
     1501 cttctctcgc accggtcatc tgcgccggcg cgctcgcaag cattcgtcac ctcaagacga
     1561 gtcaggtgga gcgcgaaatg caccagctcc gcgcggctgt actgaaagag agactggcgc
     1621 tcgagaacct gccggtaatg cacacgccaa gccatatcgt gccggtgctc gtcggcgatc
     1681 cggtgcagtg caaggctctg acggattcac tgcttgatcg gtttgggatc tatgtacagc
     1741 cgatcaacta tccaacagtt cctcgtggaa cggaacgcat ccgcctgacg ccaggcccaa
     1801 atcacaccga cgagcagatg gaccatctgg ttagttcgct tcgcgtgatg tgggacgaaa
     1861 tggggcttcg cagcggttac gagtggtgcc agctgtggcg cggacggatc gcggtcgcgg
     1921 gtcagcttga tctcgcggtt gcagcaggcg gctaagactt tccccctctg gggcatttga
     1981 aaccaccctg gcgaaagcca gggtggtttt ctattgcgtg aactccagct cgaagcgaag
     2041 gagtgcgacc ttttcgggaa gaacggaacg cttggcaaat cctcttgttt gccaaacaaa
     2101 tcggtccccc ggaggatgac aatgctggac acgcatcagc aaggctttgc gaagcttgtt
     2161 gccatgattg aagctctcga cgtggggatg ctcacgaccg aggatgaaaa cggacacctt
     2221 cggtcaaggc caatggcaac acagagggca gaggagggcg ttctctggtt cttcacaggc
     2281 aaagactcgc caaagatcga ggaagtcaaa aaagacgccc aagtgaatgt gagctacgcc
     2341 gaccccaaca accaggttta cgtttctgtc tcgggtcccg cgcgggcgtt tgtgatccgg
     2401 caaaggtccg cgagctatgg agcagcgcgg ctcagcggtg gtttccggaa ggaccggatg
     2461 atccgcatct gatgatcctt aaggtggaga tcacaggggc tgaatattgg gatgtcgaca
     2521 agcgtgcgat gatccagctt ttggaaaaag gtgcaggcgc ggcggaaatc gtgtcggcga
     2581 ccgatcataa gaaattctct tagagcctat ccgcttttga tggaatcaaa agcgggctct
     2641 aagtttttgt ttgccttgcg tctttatcgg aaaatcgctt cgccgaggcg cattccgggg
     2701 tgacgatcat cgggacgctc gcatccgatt aaacgcacga tgctctagac ttcgctgatt
     2761 ttacccgaag tccgcaataa aggatcgtgt ccgtttctaa tctgcagcat tgtcttcgcc
     2821 aggttgccat tacttgcgcg cacaaatact tgtgagtgca atcgtggact atatccttgc
     2881 caatcttcca ccgccgagct aggccttcgc cgatcatcac cattttggag acatttcgaa
     2941 tgacgcggaa ccggcttgcg atccttgccg tctcaactct tctcgcgacg accttggacg
     3001 cctcggcaga tccttcggcc tacgccttcg acccgatgca caccgaggtg cggttttgct
     3061 ggaaccatct cgggatcagc cgtcaatgcg ctcacttcac aggctacgac ggcgaacttc
     3121 tatacgatga ggccaaccct gaaaaaagtc agctcaaggt aacgttcaag acagacagca
     3181 tcgagaccct tgtgcccatt ttcaacgacc atatgaaagg cgataagctt ttcgatacga
     3241 agaaattccc ggaagcgact ttccaaagca cgaaattcga acggacaggt gaaaaagccg
     3301 ggagagtcag cggcgatttg acccttcacg gcgtcacgaa gccagtgacg ctcgacgtta
     3361 ccctcaattt tgcaggcatt cagccgatgt ccaaaaagcc aactctcgga atcggagcaa
     3421 aaacgaatgt gaaacgcacc gatttcggtg tatccgcggc cgccccttat gtgtcggatg
     3481 aggtctcaat cgagattcag accgagatgt ttaagaagag ttagattaac ccgcctttat
     3541 ccgactctat tagaggcgaa aaagtcatcg attctgcagt gctggagcag cctatccgcg
     3601 tccaccaacg cgggctgctc cagaccggca gacagggat
//
LOCUS       DATMGX010000828         3919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_204336, whole genome shotgun sequence.
ACCESSION   DATMGX010000828 DATMGX010000000
VERSION     DATMGX010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204336"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VNR65_13560"
     CDS             complement(<1..454)
                     /locus_tag="VNR65_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HWJ19745.1"
                     /translation="MISAKRITGLSLALGASLCATFPAFAAAVEGAVEKQPTNWPAIG
                     MFAGFVLMTLVITYRAAARTKSAADFYAAGGGITGFQNGLAIAGDYMSAASFLGISGL
                     VFASGFDGLIYSVGWLVGWPIVLFLIAERLRNLGKFTFADVASFRLDQT"
     gene            complement(451..735)
                     /locus_tag="VNR65_13565"
     CDS             complement(451..735)
                     /locus_tag="VNR65_13565"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HWJ19746.1"
                     /translation="MSEHPKFQELVRRRSRFAWTLSWIMLLIYQGFILLVAFAKGFLA
                     TPINGGIITIGIPIGLGVIISAFLLTGIYVWVANSRFDEMTRELIKEVTK"
     gene            complement(1230..2369)
                     /gene="zapE"
                     /locus_tag="VNR65_13570"
     CDS             complement(1230..2369)
                     /gene="zapE"
                     /locus_tag="VNR65_13570"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HWJ19747.1"
                     /translation="MSALLLERYRRLLAEGKITPDSAQALAVEKLQMLTNRLAYYTPP
                     ARTDFFSFFTRKRGEVPKGLYIFGSVGRGKTMLMDLFFQEAPVQQKRRSHFHEFMAEV
                     HARLAVARREAKDDPIVAVGASIASEARLLCLDELFVTDIADATILNRLFGAVFESGT
                     VVVFTSNSKPSDLYKGGLNRDQFLPFIHLVEHNVELLQLEAAHDYRLEQLSNSTLYFT
                     PDDDAAKDAMDELWQKLTFGEPCASETILVLGHKLHVPCACLGVARFSFADLFEKPLG
                     TQDYLALVRHYHTIFVDGIPKMGRHDQNPARRFLTFIDTLYDHRIGFVASAAVEPGEL
                     CQAGDDAEYFKRAVSRIMEMRSPAYLEQTPGASADKGASSGGSIS"
     gene            complement(2378..>3919)
                     /locus_tag="VNR65_13575"
     CDS             complement(2378..>3919)
                     /locus_tag="VNR65_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19748.1"
                     /translation="GGEEGETSASAWVAPSGVSEETSDAPAEQTTRDHFGTAGGDFEE
                     GVWSPHQDPAAVHKETVADREAPLNDGGEAYREWRAGESEANENTGKVSWFSSFRRKK
                     NLKPTDEADENAANTLTQPTAETIPFPRTNFSVAQQETETKEEQHTIDSARQAVRSVF
                     SSLGHGGPVATAAMSEDDDERAVIGPAKPHPQNGWSERLAGLSDHFKRAGETGGDQSL
                     AEADGKRSGRDLDTVEDDAWFAEKKDDASSDSDPDDALREAMRAHFLSPSKRTSVGPS
                     LRDEELAEKLETHLKSTAVTAAEEHPRPRGIAGLWRKVSPAAEEEAYEPVPAVVEEPV
                     EVKDDDTAFDQRLYREIEETREKLGEAPRRSHRGGLAVAAAWGLFLCVASGLIVGFFA
                     FRDIIADAAPGLAPLYRILGMPVTVQPLAFEAVEYEWSTSDNRPVLTVRGNVFNRANR
                     NVRVPQFFITVKDQETAHDREYSANLRINGSKIRSNERADFDIELVSPNPTVTAVELE
                     LRNVR"
BASE COUNT          820 a         1153 c         1113 g          833 t
ORIGIN      
        1 aggtctggtc gagcctgaag gacgccacgt ccgcgaaggt gaacttgcca aggttacgaa
       61 gccgctccgc gatcaggaac agcacgattg gccagcccac gagccaacca acggaataaa
      121 tgaggccatc gaatccgctg gcgaacacga ggccggaaat tccaaggaac gatgccgccg
      181 acatgtagtc gccagcgatg gcgaggccgt tctggaaccc tgtgatcccg ccgcccgccg
      241 cgtagaagtc ggctgcggac ttggtccgag cggccgcgcg ataagttatg accaacgtca
      301 tgaggacgaa gcccgcgaac ataccgatcg caggccagtt tgtgggctgc ttttcaactg
      361 cgccctcaac ggcagccgca aaggctggga acgtcgcgca aagactcgcg ccaagagcga
      421 ggctcagtcc tgtgatgcgt tttgcggaaa tcatttcgtc acctctttga tgagctcgcg
      481 ggtcatctcg tcaaagcggc tgtttgcaac ccaaacgtaa ataccggtca gcagaaaggc
      541 agaaatgatg acgccaaggc cgatcggaat gccaatggtt atgatgccgc cattgatcgg
      601 cgtcgcgaga aaccccttgg caaaagcgac cagaagaatg aacccctggt agatgagaag
      661 catgatccag gacagcgtcc atgcgaagcg cgatcggcga cgcacaagct cctgaaactt
      721 tggatgctca gacatccatg tattagattc ctgctgcacg gcttttcctc ttttaatatg
      781 tgtgccttcc ttttggccag cgaggcacgt tagtgattac tatcagctag gcacttgact
      841 tggcgcaaat ttctttctca gcctttcgtt ggatcggcag gcaaatatga agcgattgtg
      901 ggtgcggacg ccagctttgg acgcgaaaat ccttaacctg tgcaaagaag atagacttcc
      961 atccagggtt gcataagaat cgccgtaacg ttgttgggtt caggcggaaa aagccgctgg
     1021 aaaaggcggg aatggagtgg aagccatggc aatccaaagc cgcaatcacg ggaagcccgt
     1081 cgcaacgctg aggcccttga gcctcagacg ccctggtatc gactccgccg caggcaaaag
     1141 tgccttcagc gctgacacaa ccgctgcctt cgcgcccatc atgagcgaag cggttctcgt
     1201 ctgtcttcgc ctattcgatc ccgcgacggc taggaaatag agccccccga gctggcccct
     1261 ttgtcggcac tcgctccggg ggtttgctcc aggtatgccg gcgaacgcat ttccataatc
     1321 cgcgacacgg cccgtttaaa atattcggcg tcgtcgccgg cttggcacaa ctcaccgggc
     1381 tcgaccgcgg ccgacgccac gaagccgatc cggtgatcgt aaagagtatc gataaaggtc
     1441 agaaagcggc gcgcggggtt ctgatcgtgc cgccccatct tcgggatgcc gtcgacgaaa
     1501 atcgtgtggt aatggcggac aagcgcaagg tagtcctgtg tgcccaaggg cttctcgaag
     1561 agatcggcga atgagaagcg cgccaccccc aggcaagcgc aaggcacatg cagcttgtgc
     1621 ccgagcacga ggatcgtttc gctggcgcac ggctcgccga aggtcaattt ctgccagagc
     1681 tcgtccatcg cgtcttttgc cgcgtcatcg tctggcgtga agtagagcgt gctgtttgac
     1741 agctgttcaa ggcggtaatc gtgagccgct tcgagctgca gaagctccac attatgttcg
     1801 acaagatgga tgaaaggcag aaactgatcg cggttgaggc cgcctttgta gagatcgctc
     1861 ggcttcgagt tcgaggtgaa gaccacaacc gtgccagatt cgaaaaccgc gccgaaaaga
     1921 cggttcagaa tcgtagcgtc cgcgatgtcg gtcacgaaga gttcgtccag gcagaggagg
     1981 cgtgcttcgc ttgcaatgct ggcgcccact gcgacgatgg gatcgtcttt cgcctcccgg
     2041 cgcgcgacgg caagccgggc gtggacctcc gccatgaact cgtgaaagtg ggagcgacgc
     2101 ttctgctgaa cgggcgcttc ctggaagaag aggtccatca gcatcgtctt gccgcgtccg
     2161 acggaaccga aaatatagag gcccttcggc acttcgccgc gtttgcgcgt gaaaaaggaa
     2221 aaaaagtcgg tccgcgccgg aggcgtataa taagccagcc ggttcgtgag catttgaagc
     2281 ttctcgaccg cgagcgcctg ggcgctgtcg ggcgtgatct tcccctccgc caataagcgg
     2341 cggtatctct ctaacagcaa tgcgctcatg accggggcta gcggacgtta cggagttcca
     2401 gctctaccgc cgtgacggtt gggttcggcg acaccaattc aatatcaaaa tcggctcgct
     2461 cattcgatct tattttcgag ccgtttatcc tcaaattagc cgagtattcc cggtcgtgcg
     2521 cagtctcctg atcctttacg gttatgaaaa actgtggaac cctgacgtta cggttcgcgc
     2581 gattgaagac attgccgcgt acggtaagaa caggcctgtt atctgacgtg gaccattcat
     2641 attcaactgc ctcaaaggcc agaggctgca ccgtaacagg cattcccaaa atgcgataaa
     2701 gcggagccaa tccaggagcg gcatcggcga taatatcccg gaaagcgaaa aatccgacga
     2761 taagcccgct tgccacgcag aggaaaagcc cccacgccgc tgctactgcg aggccacccc
     2821 ggtggctgcg acgtggggct tcgcccaact tttcccgtgt ttcctcgatt tcgcgataga
     2881 gacgctgatc aaaagcggtg tcgtcgtcct taacttcgac cggctcctcc acgactgccg
     2941 gcacaggctc gtacgcttcc tcttcggcgg ctggggacac tttgcgccaa agcccggcta
     3001 tgccgcgcgg cctcggatgt tcttccgcgg cggtcacagc tgttgatttg agatgggttt
     3061 cgagtttctc ggccagttct tcgtcgcgaa gcgaagggcc gacggacgtt ctcttggaag
     3121 gagaaagaaa gtgtgccctc atcgcctcgc ggagagcgtc gtcagggtcg ctgtcgctgc
     3181 ttgcatcgtc tttcttctct gcgaaccaag catcgtcctc tactgtatcg agatcacgcc
     3241 cgctgcgctt gccatcggcc tccgccaagc tctgatcgcc gcctgtctca cccgctcttt
     3301 tgaagtgatc tgacaaccct gccagcctct ccgaccagcc attttgaggg tgaggttttg
     3361 caggcccgat cacagccctc tcgtcgtcat cttcagacat tgcggcagtc gcgacggggc
     3421 cgccatggcc caaactggaa aagacgcttc gaacggcctg ccgtgccgaa tcgattgtgt
     3481 gctgttcctc ttttgtttca gtctcctgct gcgcaacgga aaagttggtg cgcgggaacg
     3541 ggattgtctc cgcggttggc tgggtcagcg tgttcgccgc attctcatcg gcttcatctg
     3601 tgggcttcaa gttctttttt cgcctgaaac tgctgaacca actcactttc ccggtattct
     3661 cgtttgcctc gctctcgcca gcgcgccatt cccgataagc ctcgccccca tcgttaagag
     3721 gtgcttccct atcagcgacg gtttctttat gaactgcagc ggggtcttgg tgcggcgacc
     3781 aaacgccttc ttcaaaatcg cctcccgcag tcccgaagtg gtcacgagtt gtttgctcag
     3841 caggggcatc gcttgtttct tcgctcactc ccgaaggagc gacccaggcg gacgcgctag
     3901 tctcaccctc ttccccgcc
//
LOCUS       DATMGX010000829         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_336310, whole genome shotgun sequence.
ACCESSION   DATMGX010000829 DATMGX010000000
VERSION     DATMGX010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336310"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1051..1689
                     /locus_tag="VNR65_13580"
     CDS             1051..1689
                     /locus_tag="VNR65_13580"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWJ19749.1"
                     /translation="MPPASRTSKPTRRPDLALQRKYGGLIAGVDEAGRGPWAGPVVAA
                     AVIFIGKPPSGINDSKQLSAEEREALFPKIQATAHVGIGISAVELIDGINIYHATHAA
                     MADAIKNLACAPCAVLIDGNRYPKLDMPAEALVGGDAISISIAAASIIAKVTRDRMMR
                     ELACTFPQYAWERNKGYGTPEHAAALTLHGVTCHHRRSFRPVWERLMLEASA"
     gene            1824..>2274
                     /locus_tag="VNR65_13585"
     CDS             1824..>2274
                     /locus_tag="VNR65_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19750.1"
                     /translation="MRRLIAWTLTWAAIASLVTQAPLVAYAQSPVVVTVDECRNLSDT
                     DVRDRIRDLASTSLKTELTAIDYAALVNTYWAKAGVDTRIDQDIDDAVAFVRSDTTWA
                     DRAYSTVSRSTAARYATAVADKAYNSQGFKDALNEVATGVARDAGTKI"
BASE COUNT          516 a          666 c          622 g          470 t
ORIGIN      
        1 tccgaagtgg aagtctgtgc cgcccgagcc agcatggccg acgagggctt gtggttctgc
       61 gggaaaggaa aaaccgcgct tgtttgcgcg gttttcggtg gggcatattt acaaaaagtc
      121 tttaaaatac cacgtggtga acatcagtgg cttcacgcca ccattatact gacataactt
      181 gattacatta accgccatta cttgtcgcca caatcgctgg caggttttgg atgctgaaag
      241 actgttgctt agacagcaac gcttcgaata cgataagcgc cgcaagacga attgtaacgt
      301 ggtgtgataa cagactggtg gtgatctaat aggttggcgg cagagggatt cgaactcagc
      361 cgccccaaaa ggattcgaaa tccacagctc gatccggggg cgagagggat atcggctccc
      421 gaccacaggt cgcacgcgtg catgctggaa gggaatacaa aatacagggc ggccgttggc
      481 gaacccggca atgtaacggg gcgcgcgcgg agatctcttt ccgcacctgc tttgggcaat
      541 gacatgcgat gtccggcgaa aaaccgcgaa ggctaggcaa gagttagtta acttacaaag
      601 gctttcttga ccatgcgccc gccgcgcgcg tcaaccatta ttgcggcgcg gggcatttca
      661 acgtttttac actttaaatc aactgcttat cgagccaacc tcgggacaag cgatcccttt
      721 cggccaaagc acgcggatgc aaacgcaaag cgcaagccgg tctaatggga tccgtcagaa
      781 ctcaagcgcc gcaggctccg gcgtcctttc cggcagcaaa agtatctata taggtatata
      841 tatgcatttt accaaacaaa accaatttta accccctgta cacctcgcag ggtgacacgc
      901 ctcgaaaagc tctcatggtg gtataaggga acttcggcgg gaggcatcac aggcggcccg
      961 atcaggatgt tgttcttaag cttgggaaaa agctcgtatt tcgctctttc gccgcttgat
     1021 tgcgcgagct tggcggttca agaacaggcc atgcctcccg cctcacgaac atcaaagccc
     1081 acacgccgac ccgatctcgc ccttcagcgc aaatatgggg gcctcatcgc tggcgtcgat
     1141 gaagccggcc gtggaccttg ggcggggcct gtcgtagccg cggccgtcat tttcatcggt
     1201 aaaccgccga gcggtattaa cgattccaag cagctgtccg ccgaggaacg agaggctctc
     1261 ttccccaaaa tccaggccac agcccatgtc gggatcggta tttcagccgt ggaactcata
     1321 gacggcatca atatctacca cgcaacgcat gccgcgatgg ccgacgccat caaaaacctc
     1381 gcctgcgcgc cgtgcgccgt tctcatcgat ggcaaccgct atccaaagct cgacatgccc
     1441 gccgaggccc ttgtcggggg cgacgcaatt tccatttcga ttgcagccgc gtcgatcatc
     1501 gccaaggtta cgcgagacag gatgatgcgc gaactggcgt gcacgttccc gcaatacgcc
     1561 tgggagcgca acaaaggcta cggaacccca gagcacgccg ccgccttgac gcttcatggc
     1621 gttacctgcc atcacaggcg gagttttcgt cccgtgtggg agcgcttgat gctggaagcg
     1681 tcggcctgaa ccccgggaaa gtttagccag actttccccg accccttttc tttcacattt
     1741 gcatggctag gtaatacgca gtatctcgtc gcatgatcct caccattcca ttcacgcgtt
     1801 ctccactcag ccggggccca attatgcgta ggctaattgc ctggactttg acctgggccg
     1861 cgattgcaag tcttgtcact caagctccgc ttgtcgcgta tgcccaatcg cctgtggttg
     1921 taaccgtcga tgaatgccgc aatctgagcg ataccgacgt gcgcgaccgt attcgcgatt
     1981 tggcctcgac gagcctgaag accgagctca cagcgatcga ctatgccgct cttgtcaata
     2041 catactgggc caaggccggc gtggatacgc gcatcgacca ggatatcgac gatgcggtcg
     2101 cctttgtccg gtcggacacg acctgggctg accgcgccta ctcaaccgtc agccgttcaa
     2161 cagctgcgcg ctatgcgaca gctgtcgccg acaaagccta caattcgcaa gggttcaagg
     2221 acgcgctgaa cgaggtcgcc acgggcgtcg cgagggacgc cggaaccaag atcg
//
LOCUS       DATMGX010000830         3705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_119414, whole genome shotgun sequence.
ACCESSION   DATMGX010000830 DATMGX010000000
VERSION     DATMGX010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119414"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VNR65_13590"
     CDS             <1..205
                     /locus_tag="VNR65_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19751.1"
                     /translation="RSDRAVAVLRQNTLLEATITQDLPGQFDEIKGPQPFKIGFGLDV
                     LDHHLADCFKVASAFAWEQVARL"
     gene            complement(377..1567)
                     /locus_tag="VNR65_13595"
     CDS             complement(377..1567)
                     /locus_tag="VNR65_13595"
                     /inference="COORDINATES: protein motif:HMM:NF016302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HWJ19752.1"
                     /translation="MHRSCDTIGAIAAALAKAQGELANPEKSLSATIRSPFPREGDRT
                     FRYASLASGLDLVRKSLGQHEIATIQTTAIDQDSGQIRLTTLLAHASGEWISSDWPVC
                     AVSETATPHRMGAALTYARRYALFALVGIAGEDDLDVPDLLTEPSPVMRSGPNQPPQI
                     SRNPSNDSIHKPRHPKPVLGTEPSATLRDQLVSEIQGLKDGDDLALWAHRRLPAKNTL
                     TAGDALTVEAAYQAILNVANRDVKVQPKVLGPSSEPTSTSSENGAAVAIGSAVDGQQP
                     PTARTVFPLRKLVRRRNKAHLAFVAAQPCLICQRSPCDSHHLKFAQPRTLGRKVSDEF
                     TVPLCRDHHRDLHRYGNETAWWANVQIAPIEVARDLWQTTLLQPNTSIAKDGVGEMAM
                     TLKL"
     gene            complement(1551..2378)
                     /locus_tag="VNR65_13600"
     CDS             complement(1551..2378)
                     /locus_tag="VNR65_13600"
                     /inference="COORDINATES: protein motif:HMM:NF021128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HWJ19753.1"
                     /translation="MRPATQPNKIERRSFIGGSDARMIMGSDEAMLVRLWREKRGEVE
                     PEDLSDNLIVQLGTVTEDLNRRWFERNSGHVIKDVQRRVQHPVLEWMAATLDGLVDPG
                     GAVFEAKFMLPWTFSEEAAAEKHMAQLQHNMWVANARSAVLSIITGGGKWVEMSVHAD
                     PLYQYLLLTAEKNFWRCVMSGEEPRLFGVEPPRPRLEAVRIVDMSGSNSWAEFAATFR
                     RTRPAYQEHESAKADIKKLVPEDAKEAIGHGLRAKRSKSGAISFELMDVEVADAPLL"
     gene            complement(2413..2931)
                     /locus_tag="VNR65_13605"
     CDS             complement(2413..2931)
                     /locus_tag="VNR65_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19754.1"
                     /translation="MQYLDEALKLDVRSLMAVAMKTGKQSAYSITLSCPTGQVICRDL
                     RSSPDRVATVSITVNWPTKTGDPVSGGIFLTFRGWADRATEQTLYLSGTPAKVGGWLW
                     RATCPETRQSVQALYLAPDGDRFLSRDATGLKYRRADPGAARYLRRCFKLAQKLKTDH
                     FGPGIGKPRGIA"
     gene            3089..3670
                     /locus_tag="VNR65_13610"
     CDS             3089..3670
                     /locus_tag="VNR65_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19755.1"
                     /translation="MTTERKTDFSLIFGEPALLKGESREKYDLLRAHVAAQLNANDDV
                     FSQLRVQEVTDGIWEGHRFKRLGFQMIESAFVTALQYLLQPACAGIARSAELMAHDYY
                     FGKPEKQTLAKEIAAELGITAERILGQAIAMSGEFRMFDRLADNRATTYKGLLKDHER
                     QLRKTEKRKAAADRQREPARVNDNSAAAKKDVA"
BASE COUNT          755 a         1032 c         1107 g          811 t
ORIGIN      
        1 tcgctctgat cgcgcagtgg cggtactccg ccagaacacg ctgcttgagg ccactataac
       61 gcaggatctc ccaggacaat tcgatgagat caagggtcca caaccattca agattggttt
      121 cgggctggac gttctcgacc atcatttggc ggattgcttc aaagtcgctt cggctttcgc
      181 ctgggagcag gtcgcacggc tctgaaaacg cctgtagctc cgcgggcatc tttagccggg
      241 aaggtgaatt ggatcggttg tcagtctggt cgaccatggt cgttaactcc tggccgcggt
      301 tttttggcga gcgttggtgg tgccaaaaag atggcgtttc ggcctagtga tggaaggaca
      361 aatgcagaac gaatggttat aactttagcg tcatcgccat ttcgccaacg ccgtcctttg
      421 cgatcgaagt atttggctga agcaacgtgg tctgccagag atcccttgcc acctcgatcg
      481 gggcaatttg aacgttggcc caccacgcgg tttcgtttcc atagcggtgc aggtcccgat
      541 ggtgatcccg gcacaatggc acggtgaatt cgtcgctgac ctttcggcct agtgttcggg
      601 gttgcgcaaa cttcagatga tgcgaatcgc agggtgagcg ttggcaaatc aggcaaggtt
      661 gggctgcgac gaaagcgaga tgggccttgt ttctccgccg gactagtttg cgtaagggaa
      721 agactgttct agccgtcggc ggctgttgac cgtcaaccgc cgaaccaata gcgaccgccg
      781 ctccgttttc tgagctggtc gatgtcggtt ccgacgacgg acctagaacc ttgggctgaa
      841 ctttgacatc gcgatttgct acgttcagga tcgcttgata ggccgcctcg accgtaaggg
      901 cgtcgcccgc tgtgagcgtg ttcttcgcag gcagtcgccg atgcgcccac agcgcaagat
      961 cgtcgccgtc tttgaggcct tgaatctcgg agaccaactg atcgcgcaat gttgctgaag
     1021 gttcagttcc aaggaccggc tttgggtgcc ggggtttgtg gatggagtcg ttggacgggt
     1081 tgcggctgat ctgaggaggt tggtttggac cagagcgcat cacaggcgat ggctccgtga
     1141 ggaggtcggg gacatcgaga tcgtcctcac cggcgatgcc gaccagggca aacagcgcat
     1201 agcgtcgggc ataggttagc gccgctccca tccggtgcgg ggtagcggtc tcgctgacgg
     1261 cgcagaccgg ccagtccgag gatatccatt cgccagaggc atgcgctaga agcgtcgtca
     1321 gtctgatctg gccactgtcc tgatcgatgg cggtcgtctg gatcgtggcg atctcatgct
     1381 ggcccagact tttgcggacg agatcaagcc cgctagccag cgaggcatag cggaaggtcc
     1441 tgtctccctc gcgtggaaac ggcgagcgga tggtcgcact cagggacttt tcggggttag
     1501 cgagttcgcc ctgggccttg gccagcgccg cggcaatggc gccgatggtg tcacaggagc
     1561 ggtgcatcgg ccacctccac atccatcagc tcaaagctga tcgctccaga cttggagcgt
     1621 ttagcccgca ggccatgccc aatggcctcc ttggcatcct ccggtaccag ttttttgatg
     1681 tcggccttgg cgctttcatg ctcttgatag gccggccggg tccggcggaa ggtggctgcg
     1741 aactctgccc acgaattgga gccactcata tcgacgatgc ggaccgcctc cagccgcggt
     1801 cgtggcggct cgaccccgaa cagccggggt tcttcgccgc tcatcacgca gcgccagaag
     1861 ttcttctccg cggtgagcag caggtattgg tacagcggat cagcatgaac cgacatctcg
     1921 acccacttgc cgccgccagt gataatcgac aacaccgctg accgggcgtt tgccacccac
     1981 atattgtgct gtagctgcgc catgtgcttt tcggcggcgg cttcttccga gaacgtccag
     2041 ggcagcatga acttggcctc gaacaccgcg ccaccgggat ccaccaggcc gtctagcgtc
     2101 gctgccatcc attcaaggac cggatgctgg acccgtcgct gcacgtcctt gatgacgtgc
     2161 ccggagttgc gttcaaacca gcgccgattg aggtcctctg taaccgtacc aagctggacg
     2221 atcagattat cggaaagatc ctccggctcg acctcgccgc gcttctcccg ccacagacgg
     2281 accaacatcg cctcatcgct gcccatgatc atccgggcgt cggagccacc gatgaaagaa
     2341 cggcgctcaa ttttgttcgg ctgagtagct ggtctcattt tactctccag tgtaccgctc
     2401 caaagtgatc ggttatgcga taccgcgcgg ttttccgatt cccggtccga aatgatcggt
     2461 tttgagtttt tgcgcgagct tgaaacagcg gcgcaggtag cgagcagctc cggggtcggc
     2521 gcgccgatat ttcaggccgg tcgcgtcgcg cgatagaaag cggtcgccgt caggcgccag
     2581 ataaagcgcc tggaccgatt gcctcgtttc gggacaggtg gctcgccaca accagccgcc
     2641 gactttagcg ggcgtgccgc tgagatagag cgtctgttcc gtcgcgcgat cggcccaccc
     2701 gcgaaaggtt aggaaaatac cgccagagac cggatcaccg gttttcgtcg gccaattcac
     2761 tgtgatggac acggtagcga cccggtctgg actactgcgc aggtcgcgac agatcacttg
     2821 gccggttggg caacttagtg tgattgaata ggcgctttgc ttgcccgttt tcatggccac
     2881 ggccatcaat gaacggacat ccagtttcag cgcttcatcg agatactgca tggttacctc
     2941 caaaccggct cgggtttggt aactccgcag ccccaaggtt gaaagcgaat tattgcactc
     3001 agggagtgca tttttacaat ataacgactt gtagtcgatc tcgatcggag tgataattct
     3061 cactcgtttt caacaggagg atttggtcat gacaactgag cgcaaaaccg atttttcgct
     3121 gattttcggg gagccggctt tattgaaagg tgaaagccga gagaagtacg atttattgcg
     3181 ggcgcatgtg gcggcccagc ttaacgctaa cgatgatgtt ttctctcaat tgagggttca
     3241 agaggtcacg gacggcattt gggagggcca ccggttcaag cgtctcgggt tccaaatgat
     3301 tgagtctgca ttcgttacgg ctttgcaata tttgctgcag ccggcgtgtg ctggtattgc
     3361 gcgcagcgca gagttgatgg cgcacgacta ctacttcggc aaaccagaaa aacaaacgct
     3421 tgccaaagaa atcgcggccg aacttggcat cacggctgaa aggattttgg gccaggcaat
     3481 tgcaatgagc ggcgaattta gaatgttcga tcgcttggcc gataaccgcg cgacaacata
     3541 caaggggctg ctcaaagatc acgaacgaca gttgcggaaa actgaaaagc gaaaggctgc
     3601 agcagaccgt caacgcgaac cggctcgtgt caatgacaat tcggccgccg ccaaaaaaga
     3661 cgtcgcgtag gtaacccatg gccaccgaaa agcagattgc tgcca
//
LOCUS       DATMGX010000831         2685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_268017, whole genome shotgun sequence.
ACCESSION   DATMGX010000831 DATMGX010000000
VERSION     DATMGX010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268017"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VNR65_13615"
     CDS             <1..194
                     /locus_tag="VNR65_13615"
                     /inference="COORDINATES: protein motif:HMM:NF025051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HWJ19756.1"
                     /translation="QEAKRCAIKSKLSEGGYSVRKVQPGIGHYDVMATDNSGAKVDLD
                     VNPRTGEIMKPAGSGNEKY"
     gene            538..819
                     /locus_tag="VNR65_13620"
     CDS             538..819
                     /locus_tag="VNR65_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HWJ19757.1"
                     /translation="MLKKLSFALFFAAVSTAALAEPSDAECRVAWERADKDKNGVVTG
                     SEVTPYLIALMKRKDIYYEHAKDGKLTQDEFLKACRDGAFESINLQVWE"
     gene            984..1454
                     /locus_tag="VNR65_13625"
     CDS             984..1454
                     /locus_tag="VNR65_13625"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HWJ19758.1"
                     /translation="MRKILSIPAVAIAMAISAVALGGETVSNATRQDMLTAMKAEAFA
                     YAKYKLYSEQARKNGNEALAALFERTAADEFDKHFKEQAKYIGLVKSDHMNLVDAMSG
                     EYVEYTQMYLDMAKRAEAAGDASSARYFTEIAADESNHRDAFKAALATPSRGKD"
     gene            1959..2207
                     /locus_tag="VNR65_13630"
     CDS             1959..2207
                     /locus_tag="VNR65_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19759.1"
                     /translation="MIENYTQDNRTYPCPKCLRNSIMAIAALLHLEAAKTDEKGSGQN
                     RPEGTAPEAFAEAARERMLSVMDAVVDLDTAPGKRRLM"
BASE COUNT          702 a          674 c          763 g          546 t
ORIGIN      
        1 ggcaagaggc aaagcgctgc gcgatcaagt ctaagttgag cgaaggtggt tattcggttc
       61 gaaaggttca gccgggaatc gggcattacg acgtgatggc gactgacaat tcgggcgcaa
      121 aagtcgacct cgatgtcaat cccaggaccg gcgagattat gaagcccgcc ggcagcggca
      181 acgagaagta ttgagcgtat tgcgagacac gattcttaga aaagcgggcc gccattcgcg
      241 cgttctgagc agacttgagc aataccctct taagggagga aacggatacg gtctttgtga
      301 ccaggaatgc cgtactcgat gcgatgccag ctgccgccga tgagactctc ataggcgata
      361 aagcagcaag gaagtccgtt cgccgtccaa agatcggggc tgtcccgcat cgagctgcat
      421 caagcaacca ggaccatggg cagaaattca taatacgtag ttaagtttgt tataataggc
      481 gaaatctaga agtgtgttat aaggtttttg ataaaaatca attctaggag agtccgaatg
      541 ctgaaaaagc tcagtttcgc attgtttttt gccgctgttt ctaccgcggc tttggctgag
      601 ccatcggatg ctgaatgcag ggtggcctgg gagagggcgg acaaagataa aaatggcgtc
      661 gtaaccgggt cagaagtaac gccgtatttg atagccctga tgaagaggaa ggacatctac
      721 tacgaacacg ccaaggacgg aaagcttact caggacgaat ttctgaaggc ctgcagagat
      781 ggggcttttg agagtatcaa cctgcaagta tgggaataaa gaaggaatta aagaggcgca
      841 accgagggcc tgaagataat tcaatcagca catcacacag attgaaagag caataaatct
      901 ccgactgtgc tcgccagaaa ttgtccaaat ctgtcgtata gctatggctg gcatcgtttt
      961 aactacatcc gagggaggca catttgcgca agattctgag cattcccgct gtcgctatag
     1021 cgatggccat ttctgccgtc gccctaggcg gcgaaaccgt gtcgaacgcg acacggcagg
     1081 acatgctcac tgccatgaaa gccgaggcct tcgcctatgc gaagtacaag ctgtattccg
     1141 agcaagcccg caaaaacggc aacgaggcac tcgccgctct cttcgagcgc accgctgccg
     1201 acgagtttga caagcatttc aaagagcagg ccaaatacat aggtctcgtt aaaagcgacc
     1261 acatgaacct agtggacgcc atgtccggag aatacgtcga gtacacccag atgtacctcg
     1321 acatggcgaa gcgggccgag gcggccggcg acgcctcctc agcgcgctat tttaccgaga
     1381 tcgctgcgga cgagtctaat catcgcgatg ccttcaaggc tgcgctcgca acgccaagcc
     1441 gcggcaagga ttaggaatcg gagggctggc tccggcgatc catgcgctgt catcggcgga
     1501 atgatttcgg ccagttcgga tgacgatggc ttaagcataa agcgccaccg cgactgaaca
     1561 gcgggcgcaa ggcggtaaga aaattatccg cctcgcagcg cgccgtcagg aaattgtcag
     1621 caacgcttcg atattatccc tatttggcgg cgcttgcgct gagcggggaa ttatatgggg
     1681 ccggcgcggc aagatctgct cgccgcgaca ctgtgcggct tcgcttgcga gcccatcatt
     1741 tcgattgatt tacgtatata gcaagaaaaa tgcacgccta ttgccgtgac ctactgttaa
     1801 tattgcgtcg ataacgtaat aataaattga cccaatccat cagatcgaga agggctttat
     1861 aggaaaccat gagtgagcat gatagttgtc caaacgaccg tccggactgc gactttccag
     1921 agaaacatga gcttgcttta gctggaagaa tactgggaat gattgagaat tatacccagg
     1981 acaaccggac ctatccttgc cccaaatgtt tgcgtaattc gatcatggct atcgccgcgc
     2041 tgctgcatct ggaagcggca aagaccgatg agaaggggtc tggccaaaac cggccggaag
     2101 gcaccgcccc ggaagctttt gccgaagctg cccgggaacg gatgctctcc gtcatggacg
     2161 ccgtcgtcga tttagacacc gcccctggca aacggcgact gatgtaaccg ccgcccaaat
     2221 caatcttttg caacatcggg gggatagaga atccatgaaa attagaccaa tagcctagac
     2281 agacccgacc gcttccgctg cggtgaagca aggccgtcag gcgcgtgtaa aggacccttc
     2341 acgcgacaga cggcttgcta gaaatagatt ggggccaacg gggcagggac aagtcaagga
     2401 tgaagcaagc gacaagcgcc ttggtaggaa tagggagggt ccgaccggcc aaggacgcgt
     2461 aaagaatcct tcgcgcgaca gacggcttaa caaggagcgc tgtgacctgg gcgattaaga
     2521 agaggaatta ttcgcaggtg cgcgcctgtg cgctcgtcgc gcttttgccg aacacgtgcc
     2581 gttatcaagc acgcttgtgt tcaagagtcg gaatgttcgc gcttttgagc gggatctctc
     2641 cgctattgaa gtcgatatcg atcacgaggc ccctggcgtg cactt
//
LOCUS       DATMGX010000832         2655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_462696, whole genome shotgun sequence.
ACCESSION   DATMGX010000832 DATMGX010000000
VERSION     DATMGX010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462696"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..1334)
                     /gene="lgt"
                     /locus_tag="VNR65_13635"
     CDS             complement(447..1334)
                     /gene="lgt"
                     /locus_tag="VNR65_13635"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWJ19760.1"
                     /translation="MLGEASMDLMAIAFPFDNPVAIHLGPLAIRWYGLAYMAGLLLGW
                     LYMRRLCTDEHLWGGPSPMRAEQTDDFLFWATIGTVIGGRLGFFLFYEPGMLFSHPLQ
                     FLRVWEGGMAFHGGLLGVGLAILLFARRNRIPVRSLMDLSAAVVPFGLFFGRIANFIN
                     GEIYGRLSDLPWAVEFPARVLAKGDLFGPRHPTQLYEAALEGVVMFFLLRYFTHSRLS
                     LRSPGLTTGVFLMAYGVFRIFAEYFKEWDMAQFFTTDYFSEGVVYSLPMIALGAYFAA
                     TAKPLRSSRHAGHEAAAKA"
     gene            complement(1392..>2655)
                     /locus_tag="VNR65_13640"
     CDS             complement(1392..>2655)
                     /locus_tag="VNR65_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWJ19761.1"
                     /translation="VIVPAFLPPPQRAAHIWTNLTLFAQFVFWGIWWPFVLLSMVLVG
                     RLWCGVLCPEGALSEAVSRHGRGRSIPHWVKWKGWPFVAFASTTAYGQFVSVYQYPKP
                     VLLILGGSTLAAIAVGYSFGRDKRVWCRYLCPVNGVFGLLAKLAPVHFRVDHEAWDRA
                     PKPSSRTRLNCAPLVPVRTMRGGADCHMCGRCSGFRGAVTLARRSPGQEIVHVAGSET
                     KPWETWLIAFGLMGLATGAFHWASSPWFVAAKQAAAEWLIDRGFTWPVTAQAPWWVLT
                     SYPDLNDVMTPLDGIVLFAYLLAATLAIGGAVLLLVAAASRVIGARHQRARFHHLAQS
                     LIPVAGCGVFLGLFALTTTMLHAEGITLPFIATARTLLMAAACLWSIALAWRICGLYA
                     RNWSTRLAASGFMAAAAFVGFAAWAVRF"
BASE COUNT          631 a          786 c          842 g          396 t
ORIGIN      
        1 aacgcgaaaa gtggtccgcc gcgcctttga gcgggccgcc gatgatagac aataacctat
       61 tgaaaaagaa cagattttta tcattaacaa attggccatt aaattcaatt taatgccgga
      121 atttccgcat taactgggcg cgcaaaagtc cgttaattat gtttttggac cagcgggtta
      181 acgccgggcg cagccggaac agctgcggcg caggcggcag cgatggcgtc ccgttcggta
      241 tttttctcgc aattatctca ctattatcag gggttggagc aggctggggg tctcgttaac
      301 ctgccggcaa ggcgaattcg ctgacgatct ttcagaagaa ggtaatgcgg acgcaggtgg
      361 ccggaagaaa atgacaagag ttgccgacaa ccgcgagatg tatacaaaaa acaccggacg
      421 cccacgtccg attaagggcc gaggctctac gctttcgctg ccgcctcatg ccccgcgtgt
      481 cttgaagagc gaagcggttt ggcggtggcg gcgaaataag ccccgagcgc aatcatgggc
      541 agcgaataga caacgccctc agagaaatag tctgtggtga aaaactgcgc catatcccat
      601 tccttgaagt attcggcgaa gatgcggaag acgccatagg ccatgaggaa gacgcccgtc
      661 gtcagccccg gagaacgcag agacaggcgg ctgtgcgtga aatagcgcaa gagaaagaac
      721 atcacgacgc cttcgagagc cgcctcgtag agctgggtcg gatggcgcgg gccgaagaga
      781 tcgcccttgg cgaggacgcg agccggaaac tccacagccc agggcagatc ggacaggcgg
      841 ccatagattt cgccgttgat gaagttggcg atgcggccga agaacaggcc gaagggcacc
      901 accgccgccg agaggtccat cagcgagcgg acggggatgc gattgcgccg cgcgaagagc
      961 aagatggcga ggcctacgcc caggaggccg ccgtggaagg ccattccgcc ctcccaaact
     1021 cggaggaact ggagcgggtg gctgaacagc atgcccggtt catagaagag aaagaagccg
     1081 aggcgccctc cgatcacggt gccaattgtc gcccagaaga ggaagtcgtc ggtctgctcc
     1141 gcgcgcatcg ggctcggtcc gccccacaag tgctcgtccg tgcaaagacg ccgcatgtag
     1201 agccagccaa ggagaagccc cgccatatag gcgaggccgt accaccggat cgcaagcggc
     1261 ccgagatgga tcgccaccgg attgtcgaag gggaacgcaa tcgccatcag gtccatggaa
     1321 gcctcgccga gcatcgcttt tgcgccctct tagcaggaag gcgtgccagc cgccaaggcg
     1381 tactgaaaag cttagaagcg aacggcccag gcggcgaacc ccacgaaagc cgcggcggcc
     1441 atgaatccgg acgcggcaag gcgcgtgctc cagttccgcg catagaggcc gcaaatgcgc
     1501 catgcaagcg cgatggacca taggcatgcc gcagccatca gcagggtccg agccgtggcg
     1561 ataaagggca gggtgatccc ttccgcatgc agcattgtgg tggtcaaagc gaagagcccg
     1621 agaaacacgc cgcaccccgc gaccgggatt aggctttgcg cgagatggtg aaagcgcgcc
     1681 cgctggtgtc tagcgccgat gacgcggctg gcagcagcca caagcagaag caccgcgccc
     1741 cctatcgcca aagtagctgc gagcaaatag gcgaagagca caatgccatc caaaggcgtc
     1801 atgacatcgt tcaagtctgg atagctggtg agcacccacc agggcgcctg cgccgtaacg
     1861 ggccaagtga acccgcgatc gatgagccat tcggcggcgg cctgcttggc cgcgacaaac
     1921 cacgggctcg acgcccagtg aaaagcaccc gtcgcaagcc ccatcaggcc aaatgcgatg
     1981 agccacgttt cccaaggttt cgtctccgag cctgcaacgt gcacgatctc ctggccgggc
     2041 gagcggcgcg ccaatgtgac cgctccacgg aaaccgctgc aacggccgca catatggcaa
     2101 tcggcgccgc cgcgcatggt gcggactgga accagcgggg cgcagttgag gcgcgtcctg
     2161 gaagatggct tcggcgcgcg gtcccaagcc tcatggtcga cccggaaatg aacgggagcg
     2221 agtttcgcga gaaggccgaa cacaccattg acggggcaca gatagcggca ccagacccgt
     2281 ttatcccggc cgaaggaata gccgacggct atcgccgcga gcgtagagcc gcccaggatc
     2341 aggagcacag gttttggata ctggtagacg ctgacgaatt ggccgtaagc cgtggtactg
     2401 gcgaaggcga cgaacggcca tcccttccat ttcacccagt gcggtattga acggccccgt
     2461 ccatgccggc tcaccgcctc cgacagtgcg ccttcggggc agagcactcc gcaccagaga
     2521 cggccgacaa gcaccatgct caggagcacg aacggccacc agatacccca gaagacgaac
     2581 tgggcgaaaa gcgtgagatt ggtccagata tgcgcggcgc gttgcggcgg cggcagaaag
     2641 gccggaacga tgacg
//
LOCUS       DATMGX010000833         4319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_437173, whole genome shotgun sequence.
ACCESSION   DATMGX010000833 DATMGX010000000
VERSION     DATMGX010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437173"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VNR65_13645"
     CDS             <1..482
                     /locus_tag="VNR65_13645"
                     /inference="COORDINATES: protein motif:HMM:NF020803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HWJ19762.1"
                     /translation="SGHHQKLAIALVNVIPRQTAGLIARASGLADASGYCPIDAYTMK
                     SKADPRIFVVGDGCTPGDMPKSAFAASSQAQVAAEAIYAELFQEPQVEAEYRNICWSL
                     IDSGDSVKIGGVYKPTQQKIQQASLFVSKLDDAPDTRGINFGDSAAWYSTLTARLYS"
     gene            complement(825..1910)
                     /locus_tag="VNR65_13650"
     CDS             complement(825..1910)
                     /locus_tag="VNR65_13650"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HWJ19763.1"
                     /translation="MSFLEDNEEYESWLAKQCDVVKRDLKKKHKRMSESPFRFLRATY
                     FRWARKIEELLPALADTPQVLAVGDAHLENFGTWRDAEGRLVWGVNDFDDAALMPYAF
                     DLVRLTTSAKLSPDVAQPDEAAAAILAGYARGLETPHPVIVDEHAAWLYPVVTPSPSK
                     REEFKEEIKADPECQPPAQAVDGFIKSFPPGVTIDGYATMSKGGGSLGRPRFAAVGEW
                     RGGRTVREAKALVPSGWDWAHKSEGASQFLKLAQGAYRSPDPFLCVHNGFVFRRIAAD
                     TGKIDFHAQIPEGLQSMLLEAMGQDLGSIHAASAVGLQIASHLKTLPAEWLQDAANKA
                     KSFVQKDFAEWLEAHPKLVPDQNGSVA"
     gene            2392..2988
                     /locus_tag="VNR65_13655"
     CDS             2392..2988
                     /locus_tag="VNR65_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HWJ19764.1"
                     /translation="MLVESGGGAMPYEIERKFLVRDDSWRASAGGAISIRQGYLTRNG
                     TMSLRIRLIDGVRATLTIKSPVSEIRRLEFEYEIPMTDGAALIGLREGGVIEKLRHKI
                     PWQGLVWEVDLFQGENRGLVIAEIELPHEDTIFEKPEWLGREVTSDPRYSNAHLAKMP
                     FRGWTDPMQDAHILGQGNGCANQSPELQAQCSGTRRNR"
     gene            complement(3124..>4319)
                     /locus_tag="VNR65_13660"
     CDS             complement(3124..>4319)
                     /locus_tag="VNR65_13660"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWJ19765.1"
                     /translation="RLSEESSQGTGSDTLQLIRSVHEHTSENLGIDQHICAQDLRALE
                     LLVKESRGVPLDAKRLDLFQSFGERMCAKIVAAKLSKDGIAARAYNAWDIGMITDDHF
                     GAAEPLASSYALIKEAIAKVNEVPVVTGFIGKTEAGEVTTLGRGGTDYTTSIIGAAIG
                     AETIQIWKEVDGIMTTDPRIVPEARVVPELAFEEACELAYFGAKVLHPKTIMPAMKAH
                     IPVEVRNTFKPELPGTTIVSTFAERANKSATLEALTIKRRVTVLHIYSPEFFEGSGLM
                     ARIFSALEKRRISVDVIATSVASVSLTIEHDEHLEEIAKELSGIGEVSIEAHKAIVCA
                     VGGSVNAAGVVGQMFSVLGDAGVAISVEMISQAAGGVSITFVVDEKDAEQAVKVLHKK
                     YIERP"
BASE COUNT          928 a         1245 c         1188 g          958 t
ORIGIN      
        1 catcaggcca tcaccaaaaa ttggcgatag ctctggttaa tgtcattccc cgccagacgg
       61 ctggacttat agcgcgcgcc tctggcctcg ccgacgcaag cggatactgc cctatcgatg
      121 cgtacaccat gaaatcgaaa gcggatccgc gcattttcgt ggtgggcgat gggtgcacac
      181 caggagatat gcccaaatcc gcgttcgcgg ccagttcgca agcacaggtc gcggctgaag
      241 cgatctacgc cgagcttttt caagaaccgc aggtcgaggc agagtaccgc aatatctgct
      301 ggagcctgat cgatagcggc gacagtgtca aaatcggggg cgtctacaag cccacgcagc
      361 aaaagataca acaagccagc ttgttcgttt ccaaactcga cgatgccccc gacacgcgcg
      421 gcattaactt cggagactcc gccgcttggt attccacatt aacggccagg ctgtattcgt
      481 aagtggggtt tgaacgcgcg ggtttgacct gccgagacta acgttcaaat accaccggcg
      541 aaaatgtcct cctcagggcc ctgtatagcc cgccagagca tcgagaaagt ttcaaagccg
      601 cgaccaaggc tgaggccgat gcgtccaagg agcacttcga cggcaagacg acggaatgat
      661 tcatagttcc gccgccctgt gcactcgtcc ggtcaaatca tctcgcggca ctggccggaa
      721 cgacaagtgt tagcgcccat gcagtgatgg ctgcttcaca caacaacgac ccccattggg
      781 ggaggcctgc aggtttgggg cagccggttg tgtcaccaga tggatcaagc gaccgatcca
      841 ttttgatcgg ggaccaactt gggatgagct tccagccact cggcgaagtc tttctgaacg
      901 aaagacttcg ccttattcgc cgcatcctga agccattctg ctggcaaggt cttcagatga
      961 ctggcaattt gcaaacctac tgcacttgcc gcgtggattg atccaaggtc ttgccccatc
     1021 gcctctagaa gcatcgactg aaggccctct gggatttgcg cgtggaaatc gattttgcca
     1081 gtgtcagccg caatgcgccg gaatacgaag ccgttgtgca cgcacagaaa tggatcgggc
     1141 gaccggtaag ctccctgggc caacttaagg aactgcgaag ctccttcact cttgtgcgcc
     1201 caatcccatc cagacggaac gagggccttg gcctcacgaa cggtacgacc gcctcgccat
     1261 tcgccaacgg cggcaaaacg aggccggcca agacttcccc cgcctttcga catagtggcg
     1321 tatccgtcaa tcgtgacgcc tggagggaag ctcttgatga acccatctac ggcctgggcg
     1381 ggcggctgac actcgggatc ggccttgatt tcctctttga attcctcacg cttcgacggc
     1441 gatggggtta cgacgggata cagccatgcg gcatgttcgt cgacaataac gggatgtggc
     1501 gtttctagcc cccgtgcata ccccgctaga atggccgctg ccgcttcatc gggttgagcg
     1561 acgtcgggag acagcttcgc gctcgtggtc aaccggacga gatcgaaggc atatggcata
     1621 agcgccgcat catcaaaatc attgacgccc caaacaagcc gtccttccgc gtcacgccag
     1681 gttccgaagt tctcaagatg ggcgtcgccg actgcgagca cctgcggcgt atcggcaagt
     1741 gctggcagaa gctcttcgat ctttcttgcc caacggaaat aggttgcacg caggaaacgg
     1801 aacgggcttt ccgacatgcg cttgtgtttc tttttgagat cgcgtttgac gacatcgcat
     1861 tgtttcgcaa gccagctctc atattcttcg ttatcttcga gaaacgacat ggttgacgac
     1921 ctctcctcat cgacaggcaa ctcggacgct ggctccttaa catctgtttt tctaagcgta
     1981 attcaatggg atgttgccgg tgcaagcaaa tagccccgga gcttgcatca ttgccgcccg
     2041 gtaggctgtc acataaacac cttagaagta agttgtggcc gagccgctta ttctgaccga
     2101 cgagattatt ggcgagcgta aagagttcgt cccgctcgcc cttcgcacaa cgtctcaagc
     2161 tgtggttgtg atatggctgg tatataaact acccgcgaac ttcaaccctc aaacgcgatg
     2221 ccgtttgttg ggtttgcgag gttgcaacta gactatggcg ctagttgaaa accgcgcgac
     2281 catccagcat attagttatc cgggaaaatt ggtgggtggc ggtttcgtaa ttggaagcct
     2341 gcgcatctgc caaaatgatc tctgccgcgg gaagctctca ataagcggtg cgtgttggtc
     2401 gaaagcggcg gcggagcgat gccttacgaa atcgaacgga aattccttgt tcgggatgac
     2461 agttggcgtg cgtctgcagg tggcgcaata tccattcgcc agggctatct cacgcgtaac
     2521 ggcaccatgt cgcttcgcat ccggcttatc gatggggttc gagccacact tacgatcaaa
     2581 tctcctgtat cggaaattcg tcggcttgag ttcgaatacg agatcccgat gacagacggg
     2641 gccgctttga tcggcttgcg agagggcggg gttatcgaga agcttcggca caagattccg
     2701 tggcagggct tagtttggga ggttgacctg tttcaggggg agaaccgagg cctggtcatc
     2761 gccgaaattg agcttcccca cgaagacacg atttttgaga agcctgagtg gcttggccgg
     2821 gaagtaacgt ccgacccgcg ctactcgaac gcccacctag ccaagatgcc gttccgaggg
     2881 tggacagatc cgatgcaaga cgcgcatatc cttggccagg gaaatgggtg tgctaatcag
     2941 tctccggagt tgcaggcgca gtgtagcggt acacgacgga atcgatgatc tgattgcggc
     3001 ttcttaagtg gcggggcgga agggcctcct caggctacaa ccctgctttg gctgcgttgc
     3061 gactcgttgc tcccataacg ctgtctctcg ggttaagtct tagctaatgc agcttccact
     3121 tcctcatgga cgctcgatat acttcttatg caataccttg accgcttgtt cggcatcctt
     3181 ttcatcaaca acgaaggtga tgctgacgcc gccagcggcc tgggaaatca tttccaccga
     3241 aatcgcaacg ccggcatctc ccaagacgct gaacatctga ccgacaaccc ccgccgcgtt
     3301 cacgctgcca ccgacggcgc agacgatcgc tttgtgcgct tcaatcgaca cctcgccgat
     3361 gccggagagt tccttcgcga tctcttcaag atgctcgtca tgttcgatcg tgagcgagac
     3421 gctcgcgacc gacgtcgcga tcacatcgac actgatccga cgcttctcca aggcgctgaa
     3481 gatgcgcgcc atgaggccgc ttccttcgaa aaattcgggc gagtagatgt ggagcaccgt
     3541 cacgcgccgc ttgattgtaa gcgcctccag agtcgcgctt ttattggcgc gctccgcgaa
     3601 ggtcgagaca atcgtcgtgc ctggaagctc gggtttgaac gtattgcgca cctccaccgg
     3661 gatatgcgct ttcatcgcag gcatgatggt tttgggatgc aggacttttg cgccgaagta
     3721 agccaactcg catgcctctt caaaggcaag ctccggcacg acgcgcgcct cgggtacgat
     3781 gcgcggatcg gtcgtcatga tgccgtcgac ttctttccaa atttggatcg tctccgcacc
     3841 aatcgcggca ccgatgatgg atgtcgtgta gtcggtgcct cctcggccta aggttgtcac
     3901 ctctcccgct tcggtttttc cgatgaatcc cgtgacgacc ggcacctcat tgactttcgc
     3961 aatcgcttct ttgatgagag cgtacgacga agccaaaggc tccgcagcgc caaaatgatc
     4021 gtcggtgatc atgccgatat cccacgcatt gtaagcgcgc gcggctatgc catccttcga
     4081 taacttcgcc gcgacaatct tcgcgcacat gcgctcgcca aacgactgaa acaggtcgag
     4141 acgcttcgca tcaagcggca cgccccgcga ctccttcacc aacagttcga gtgcgcgcaa
     4201 atcttgtgcg cagatatgtt gatctatgcc aaggttctca ctggtatgtt cgtggactga
     4261 acgaataagc tgcagcgtat cgcttcccgt tccctgcgac gattcttctg agagccgtc
//
LOCUS       DATMGX010000834         4202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_440478, whole genome shotgun sequence.
ACCESSION   DATMGX010000834 DATMGX010000000
VERSION     DATMGX010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440478"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VNR65_13665"
     CDS             <1..1206
                     /locus_tag="VNR65_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWJ19766.1"
                     /translation="NHLLFFRVDQFSGIALGVLRDIDERHSLVAILGINSYVFDIMPF
                     ELRAHSEIILELNNGSDIGRFSLKPPTEFSQLVSFNSVSSRYPLHATIRVERNALQRW
                     SGEAYWPILLIALGLGAAFGLLLTRTTTRLEGPISDIDRGLARREFKPFYQPTFDLRT
                     GTILGCEVLARWIREDGTVIPPMSFIPLAESSGRIEPMTWQILETALEELYPRLREDK
                     QFKLSINVVPRHMLSDGFIETLRRVVVDAKVSARQIVLEITERNELPDLDRAAAVVKD
                     LRELGFRVAMDDVGVGHSGLSQMKGLGANTIKIDKFFVDTITESGSAATIIETLARLA
                     RDLHMTVIAEGIETVEQVQALISCGVEEGQGYIISPPLPFRKFDELLEIRRSRVFAEA
                     VVRQAALVA"
     gene            complement(1211..1642)
                     /locus_tag="VNR65_13670"
     CDS             complement(1211..1642)
                     /locus_tag="VNR65_13670"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HWJ19767.1"
                     /translation="MKNAIYLIAAIALIVGAPNGVPSAQAETITLKADLKGTNEVPPN
                     ASSATGKAEAKLDAETRVLTWTVTYADLTGPAVGAHFHGPGEPGKNAGIVLPFKTAQS
                     PIEGTATITETQAADLLAGKWYANIHTAANPGGELRGQMMK"
     gene            complement(2042..2320)
                     /locus_tag="VNR65_13675"
     CDS             complement(2042..2320)
                     /locus_tag="VNR65_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19768.1"
                     /translation="MQLRLADDRDEPQGGGRTAGRVDPAGNDGLLIRPHRRLQVSPDS
                     GDCAVRCRDRGDAEELIGGDAPISSAVSAPMRNAFPMKPYETLSVSQP"
     gene            2371..2694
                     /locus_tag="VNR65_13680"
     CDS             2371..2694
                     /locus_tag="VNR65_13680"
                     /inference="COORDINATES: protein motif:HMM:NF017815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HWJ19769.1"
                     /translation="MGTWKSPLAALASPGDCKSYAVTKYAALGAAGIVPDDRRLVMVW
                     DNARPTQTHLIVVVRVDQRWLILDSRPLIMVDSSRAPAYQPLLTLDHSGVREFPPLPP
                     VGGPL"
     gene            complement(2720..3787)
                     /locus_tag="VNR65_13685"
     CDS             complement(2720..3787)
                     /locus_tag="VNR65_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ19770.1"
                     /translation="MSLTVTIVAFAIVFGVLLATTAFGIGWIEAAHPPVGQFIDLESV
                     QLHVAELGLARGRPGAEPAVVLIHGASGNMEDMRLALGEKLAESHRVILIDRPGQGWS
                     SRPGGDIFASPARQAEVVAEALEQLGVRRAILVGHSWGGAFVTSYALAFPDRTAGLVL
                     LSAVTHPWPADLDWYNNLASLPFVGPLFLQTLVYPVGFFLIDGISRSVFAPQAVPEDY
                     VRHAAIRLVLRPKTFFANARDLVLLKNFIATQVPRYVDLRAPTIIITGDRDAIVPPEI
                     NAYAFAATLPRAKLVVLKGVGHMPHHAAPEAVAAAIDELGRRYFYYRARQRRTSKSRM
                     GRAMSVHLSRGDIHQRDRHVG"
BASE COUNT          910 a         1193 c         1225 g          874 t
ORIGIN      
        1 aatcaccttc tattttttcg ggtcgatcag ttcagcggaa tcgctcttgg tgttctcagg
       61 gatattgacg agagacattc cctcgtcgca attctcggga tcaactctta cgtatttgac
      121 atcatgccct tcgaattgcg cgcccatagc gaaataatac ttgagctgaa caacgggtcc
      181 gatattggtc gattctctct gaaacctcct accgagttct cgcaactggt cagcttcaac
      241 agcgtttcta gccgataccc tctccatgcg acgatccgcg ttgaacgtaa cgctttgcag
      301 cgatggagcg gtgaggctta ttggccaata ttgctgatag ctttggggtt aggagcggcc
      361 tttggtctgc tcttgacaag aacaacgacc agattggaag gcccaataag tgatatcgat
      421 cgagggctag ctcggcgcga atttaagcca ttctaccaac cgacctttga tctgcgcacc
      481 ggcaccattc ttggatgtga agtgttagct cgatggatac gcgaggatgg caccgtcatt
      541 cccccaatga gctttatccc gctcgcagaa tccagcgggc gcatcgagcc aatgacatgg
      601 cagattctag aaactgcgct tgaggaactg taccctcgtc ttcgagaaga caagcaattc
      661 aaattgtcta tcaacgtggt gccccgccat atgcttagtg acggtttcat cgagacgctc
      721 cgtcgcgtcg ttgtagacgc caaagtttcg gcgcgtcaga tcgttcttga aattacggag
      781 cgcaatgaac tgcctgacct cgaccgggcc gcagcggtgg tgaaggactt gcgcgaactt
      841 ggctttaggg tagcgatgga cgacgttgga gttgggcaca gcggcctgtc ccaaatgaag
      901 ggacttggtg ccaacacaat caagatcgac aaattctttg tcgatacgat cacagaaagt
      961 ggatcagccg cgaccatcat tgagacgctc gcgcggctcg cgcgagactt gcacatgacg
     1021 gttatcgccg aagggatcga aaccgtcgaa caagttcagg cgctcataag ctgtggcgtc
     1081 gaagaggggc aaggttatat catttcgccg ccactgccgt tcaggaagtt cgatgaattg
     1141 ctggaaattc gtcgatcaag agtgtttgcc gaagcggtag tccggcaggc cgcgctggtc
     1201 gcctagcatc tcatttcatc atttgaccac gcagctcgcc tccgggattc gctgcagtgt
     1261 ggatgtttgc ataccatttg ccggcgagga gatcggccgc ctgggtctcg gtaatcgttg
     1321 ccgtcccctc aatcggactc tgcgcggtct tgaaaggcag gacgattccg gcgttcttgc
     1381 ccggctcacc gggaccatga aagtgagcgc cgacagccgg cccagtcaga tccgcgtaag
     1441 taacggtcca agttaaaact cttgtctcgg catctaactt cgcctcggcc tttcccgtgg
     1501 ccgagctagc gttaggtgga acttcgttgg tgccctttag atctgccttt agggtgatcg
     1561 tttcagcttg agcagacggt acgccgttcg gagctccgac aattagtgca atggctgcaa
     1621 tcaaatatat ggcgtttttc atggtgtcct cccggcgaat tgccctaata gcacattaac
     1681 accggacgca gcgggaaatt tcaaccggga gattgcgtgc aggcagggcg gacgcggctc
     1741 agcgaatgcg cgggcgtgct gtcttcaggc gcccagccta cccatgcccc accgccttgg
     1801 cggccgagca actctatgac tgttcctggc acttttcgga agtcgtcggt caaactgacg
     1861 acgtccgttt ttgggggcaa agcggaaaac atatactcgc gttgagcttt tcagctttta
     1921 tcggcccgtt ttgaagaaaa gcagcaggct ggcattcggc aaccaagcca gcgaacgcag
     1981 ccatgacggc gcgggctgca atcggcaatc gaaaatgccg gatcaggtcc attgatccgc
     2041 gtcaaggctg ggagactgat agcgtctcat aaggtttcat gggaaacgcg ttccgcattg
     2101 gtgcactgac cgcgctgctg attggcgcgt caccgccgat gagctcttcg gcgtcgccac
     2161 ggtcccggca ccggacggcg caatctccgc tatctggcga gacctgcaac cggcgatgcg
     2221 gacggatgag cagaccgtca ttgcctgccg ggtcaaccct gcctgcggtt cgcccgccgc
     2281 cctgcggttc atcgcgatcg tcggccaggc gaagctgcat gaagggctcg cacgggtcgg
     2341 ccactagcga tccaagcgca gcgcgacggc atgggaacgt ggaagtcgcc gctagcagcg
     2401 ctggcgtcgc ccggcgactg caagagctac gcggtgacaa aatacgccgc gctcggtgcc
     2461 gctggcatcg taccagatga ccggcgactt gtgatggtgt gggataacgc ccgcccaacg
     2521 caaacccacc ttatcgtcgt ggtgcgggta gaccagcgct ggctcatcct cgacagccgg
     2581 ccattgatca tggtcgatag ctcccgcgcg ccagcctatc agccgttgct gacgctcgac
     2641 cattcagggg tgcgcgaatt cccaccattg ccaccggtcg gcgggccact ctaaaattgc
     2701 gacttccacg tttggggact taaccgacat gccgatcgcg ctgatgaatg tctcctcttg
     2761 agagatgaac agacatggcg cggcccatcc gcgacttgct ggtccttcgc tgccgggcgc
     2821 ggtagtagaa atacctgcgt ccaagttcat ctattgctgc ggccacggcc tcgggtgcag
     2881 catggtgcgg catgtgtccg acgcctttta gaacaaccaa cttcgcgcga ggtaaggtgg
     2941 ccgcgaatgc gtacgcattt atttccggcg ggacaatggc gtcgcggtcg ccggttatga
     3001 tgatggtcgg cgcgcgcaga tcgacgtagc gcggtacttg ggtggcaatg aaattcttga
     3061 gcagcacaag atcacgcgcg ttggcgaaga atgtcttggg ccgaagcact agccggatgg
     3121 cagcgtggcg cacgtagtct tcaggcaccg cctggggcgc aaacacacta cgcgagatac
     3181 catcgatcag gaagaatccc acgggataaa caagcgtttg caggaacaac gggccaacaa
     3241 agggcaacga cgcgaggttg ttataccagt cgaggtcagc gggccaagga tgggtaacgg
     3301 cggatagcaa gacaagaccc gcagtgcggt cggggaacgc aagcgcgtag gacgtcacaa
     3361 aggccccacc ccaggaatgg cccaccagga tcgcgcgcct gacgccgagt tgctcaagcg
     3421 cctctgcgac gacctccgcc tggcgtgcgg gcgaggcaaa gatgtcaccc ccaggccgcg
     3481 aactccaacc ttggccgggg cgatctatca gaatcacgcg atgcgattcc gcaagcttct
     3541 cgccgagcgc aagccgcatg tcttccatat ttccgctcgc gccgtggatg agaaccactg
     3601 cgggctcggc gccaggtcgg ccccgcgcaa gaccgagttc ggccacgtgc agttgcacac
     3661 tctcgagatc tatgaactgg cccactggtg gatgcgcggc ttcaatccac ccaatgccga
     3721 acgcggtcgt agccaagagc acaccaaaca caatggcaaa tgcaactatg gtgaccgtca
     3781 gtgacattgt ccgctccaga atccttcaaa ataatggcgc gatggctgga tcgccttgag
     3841 ctatgtcaac gtcccgggac aaagcggggg ttcagatcga gctcccgcaa cttctccaga
     3901 gatcgccgca tggcggcgtg aatccagttg tcttgattgc cgaatgcgcc tccgtcattg
     3961 gattaggcta cgagacgctc ctgaaggcac atagtggaag ccggaagccg tcatggggcg
     4021 cagtgggtct aaaaaagtaa tcccaaaggg cttgtcatcc tcggtgaaag aaatcaggat
     4081 tagctgaaac ttgaggcggt tctttacccg gagcgtcgag cgaaacgagg tggctacctc
     4141 agagctgggc gacttccgca gtgactttca aaaccacgca cctaatttgg caggtgagtt
     4201 ta
//
LOCUS       DATMGX010000835         2207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_340612, whole genome shotgun sequence.
ACCESSION   DATMGX010000835 DATMGX010000000
VERSION     DATMGX010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340612"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VNR65_13690"
     CDS             complement(<1..803)
                     /locus_tag="VNR65_13690"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWJ19771.1"
                     /translation="MALVEDSSASHVSRVGSAASEGETALRVGLAAIAVLILLRFALA
                     ATANLAEDEAYYWLWSTHLASGYYDHPPMIAYWIRAGTAIFGQTEFGVRFIGLLSAIA
                     GSYLLYKTSLSLFRDRNAAWLCVFWMNATLLCNAAAIVATPDTPLALFTTAALFALAK
                     LIETRRGAWWYAIGASLGLAFMSKYTAALLLPGLFLWMIASAEGRRWFLQPEPYIGAV
                     IAGLIIAPVVSWNYAHDWASFAKQAEHSVKDIPANALLSVLEFFGGQAGL"
     gene            951..1988
                     /locus_tag="VNR65_13695"
     CDS             951..1988
                     /locus_tag="VNR65_13695"
                     /inference="COORDINATES: protein motif:HMM:NF018017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HWJ19772.1"
                     /translation="MTELTPHKTDTGLTWPHPLPTCWCLTDGRPGMENQAVGLAEALG
                     LTPVVKRVILKTPWRIASPHITAFKRYAFSKKGDPVDPPWPDILIATGRPSILPSLYV
                     KGQSGGRTFTVQLQDPISLRWRFDRIVVPAHDDLSGENVLVMNGALHRITPQKLADEA
                     PRWAEAFAAIPKPRIAVLLGGDNSRYRFTEAEARVLARQLKALAGQGYGLLITGSRRT
                     GESNLNVFREALAGCAAFISDGSGDNPYFGMLAHADAFIVTCDSVNMITEACSTGKPV
                     HVVPLPAQGRRVSERDKFSRFLRGLEESGRIRFFKGEIETWAYEPLREMERIASLIQQ
                     AYLSRQWPTML"
BASE COUNT          479 a          667 c          645 g          416 t
ORIGIN      
        1 aggccggcct gccccccgaa gaactccaga acgctcaaga gcgcattcgc gggtatgtcc
       61 ttgacgctgt gctcagcctg tttggcgaag gaagcccaat cgtgggcata attccaggag
      121 acgacaggcg cgatgatgag cccagcgatg acggccccga tatagggctc cggctggaga
      181 aaccagcgcc gtccctccgc cgacgcgatc atccatagga acaaacccgg caggagcagc
      241 gcagccgtat atttgctcat gaaagcgagg cccaggcttg cgccgatcgc ataccaccag
      301 gcgccccgcc tcgtttcgat gagcttcgcc agcgcaaaca gggccgcggt tgtaaagaga
      361 gcaagcggcg tgtcgggggt ggccacgatc gccgcggcat tgcagagcag cgttgcattc
      421 atccagaaaa cgcaaagcca ggcggcgttg cggtcacgga aaaggctgag gctcgttttg
      481 tagagaaggt acgatccggc aattgcgctc agcaatccga tgaagcgaac gccgaactcc
      541 gtctgcccga aaatagccgt tcccgccctg atccaatacg caatcatcgg cggatgatcg
      601 taatatcccg aagcgaggtg cgtcgaccaa agccagtaat aggcttcgtc ctcggcgaga
      661 tttgctgtcg ccgcgagcgc gaatcgcaag agaatgagaa ctgcgattgc ggcaaggccg
      721 acacgtagcg cggtctcgcc ttcggacgcg gccgagccaa ccctgctgac gtgtgatgcc
      781 gaactgtcct cgaccaaggc cacttgcgaa tcccccgttg atgctggccg atggcacaga
      841 cagaaaaccg cttcgtggca aaagcgcggc tttctctctt gcaggacgga ctttcgttca
      901 tcgtagcgcc gtggtagggc ggagcgcatc cagcaactgg aaacgactcg atgactgaat
      961 tgacgcctca caaaaccgat acaggtctaa cttggccgca tcccttgccg acatgctggt
     1021 gcctgacgga tggccggccc ggcatggaaa atcaagccgt cggtctcgcg gaagccttgg
     1081 ggctcacgcc tgtcgtaaag cgggtgattc tcaaaacgcc atggagaatt gccagcccgc
     1141 acatcaccgc tttcaagcga tacgctttca gcaagaaagg cgatcccgtc gatccgccat
     1201 ggccggatat cctgatcgcc acgggcaggc cgagcattct gccatctctt tacgtgaagg
     1261 ggcaaagcgg cggacgaacc ttcacggtgc agcttcaaga ccccatttcg ctgcgctggc
     1321 gattcgaccg catcgtggtt ccggctcacg atgacctttc cggcgaaaac gtgcttgtga
     1381 tgaatggcgc gctccaccgg ataacgccgc aaaagctcgc agacgaagcc ccgcgctggg
     1441 ccgaagcctt tgccgcaatc ccaaagccca ggatcgctgt gctacttggc ggcgacaact
     1501 cacgctacag gtttaccgaa gctgaagccc gcgtgcttgc tcgccagctc aaagccctcg
     1561 ccggacaagg atacgggctt ctgatcaccg gatcgcgccg cacgggcgaa agcaatctca
     1621 acgttttccg cgaagcgctg gccggatgcg ccgcattcat ttccgatggc agcggcgaca
     1681 acccctattt cggcatgctg gcccatgccg atgcgttcat tgtcacctgc gattccgtga
     1741 atatgatcac cgaagcctgc agcaccggta aacccgttca cgttgtgccg ctccccgcgc
     1801 aagggcggcg ggtcagcgag cgggacaagt tttcacgctt cctgcgcggc ctcgaagaaa
     1861 gcggacgcat ccgctttttc aaaggcgaga tcgagacctg ggcttatgaa cctttgcgtg
     1921 agatggagcg catcgcgagc ttgatccagc aggcctactt gtcgcgccaa tggcctacga
     1981 tgctttgaca aacaaggccg agaagagagt gatcaattct cctgttcgga atggcccgaa
     2041 cagactcagg ctgagttgtc atttggcttg tctcttgaaa agaatttctt cgacataaag
     2101 cgtttttgtg cctgggggca gatgcagact cgcgccacgc gcttgctttc ctaaaggagc
     2161 cgctagggcg gcgtgcgctg aatcggacgc gcgcgtccga tgttttt
//
LOCUS       DATMGX010000836         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_176823, whole genome shotgun sequence.
ACCESSION   DATMGX010000836 DATMGX010000000
VERSION     DATMGX010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176823"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1083
                     /locus_tag="VNR65_13700"
     CDS             85..1083
                     /locus_tag="VNR65_13700"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ19773.1"
                     /translation="MASEMAQLIGTTRNPVPNGAVAGAFNARGGVRIRYARWDTTAER
                     RLGTVCLFHGRAEFIEKYFETITDLRRRGFAVATMDWRGQGGSSRLLRNPSKGHAENF
                     SQFDGDVRQFMSEIVLPDCPPPYYCLAHSMGGHLVLRLAQTKVCWWDRMVLTAPKIQF
                     AGTDSQKTWTCTAAEAAALIGFGDCFIPGGKPHLWEDVAFEGNPVTSDRLRHERAQEV
                     LRAAPHLAIGSPTIGWVAAACNSISIVNSSAFMESVKVPILIVAAANDAFVCTATIER
                     FASRLKNCAHIVVAGAKHEILQERDQYREQFWAAFDAFVPGSILTPRRSEQAEVVR"
     gene            complement(1276..>2122)
                     /locus_tag="VNR65_13705"
     CDS             complement(1276..>2122)
                     /locus_tag="VNR65_13705"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HWJ19774.1"
                     /translation="IVEIIRRYTREAGVRSLEREIAKLARKAVKLIESGKETQVHVTG
                     ETVRDFLGVQKHRYGEAELEDQVGVVTGLAWTEVGGELLTVEGVMMPGRGRMNVTGNL
                     KDVMKESIQAANAYVRSRAVAFGIEPPMFEKKDIHVHVPEGATPKDGPSAGVAMVTAI
                     ISVLTGIPVRRDVAMTGEITLRGRVLPIGGLKEKLLAALRGGIKRVIIPEENAKDLAE
                     IPDNVKNALEIIPVARMDEVLGIALVRKPEPIEWDEAAALAAAQLQNLSTQNAADGDG
                     AALPH"
BASE COUNT          388 a          678 c          613 g          443 t
ORIGIN      
        1 cgggaagctc ggctttcaag gggggcttga gcatccctgc gcaggtgaag tatgaatttt
       61 ttcatcgatg aggaggagcc gcaaatggcc tcagaaatgg cgcagctgat cggcacgacc
      121 cgcaatccgg tcccgaatgg agccgtcgcc ggcgccttca acgcccgcgg aggggttcgc
      181 atccgctacg cgcggtggga cacgacggct gagcggcggc tcggtaccgt gtgccttttc
      241 catggccgtg ccgaattcat cgagaagtac tttgagacga tcaccgacct gcgccggcgc
      301 ggctttgcgg ttgcgaccat ggactggcga ggccagggag gctcgtcgag actcctcaga
      361 aatccatcga agggccacgc cgagaatttc tcgcaatttg acggggatgt tcgccagttc
      421 atgagcgaaa tcgtactccc cgactgcccg cctccctatt actgccttgc ccactccatg
      481 ggcggccatc tggtcttgcg tcttgcccag acaaaggtct gctggtggga tcgcatggtg
      541 ctcacggctc ccaaaattca gtttgccgga accgattcgc agaaaacctg gacctgcacc
      601 gccgccgaag ccgccgccct tatcggtttt ggtgactgct tcattccggg cggcaagcct
      661 cacttatggg aggatgttgc cttcgaaggc aacccggtga cttcggacag gcttcgccat
      721 gagcgcgccc aggaggtgct tcgcgctgcg cctcacctgg caatcggctc tccgacgatc
      781 ggctgggttg cggctgcctg caattccatc tcgatcgtca actcctccgc cttcatggaa
      841 agcgtcaaag ttcccattct gattgtcgca gccgcaaacg acgctttcgt ctgcactgcg
      901 acgatcgaac gcttcgcaag ccgccttaag aattgcgctc acatcgtcgt cgcgggggcc
      961 aagcacgaga ttcttcagga acgcgatcaa tatcgcgagc agttctgggc tgcgttcgat
     1021 gcgttcgtcc ctggctcaat cctcactcct cgccgctcgg agcaggcgga agtggttcga
     1081 tagcaggcat gaaaaaaggg atgaggcagg ctcatccctt ggttcgttac tcgagcactc
     1141 gtcatcccgg ccaagcgaag cgccgagccg ggatcgtaaa aaatcaggcg cttgaaagcg
     1201 ttacggtccc ggatagggct ccgcccttcc gggatgacgc ctgcggcgat ccgttgacgc
     1261 gcccgaacac tttgcttagt gaggcaaggc tgctccgtct ccgtccgcag cgttttgcgt
     1321 tgaaagattc tgcaattgag cggcggcgag tgccgcagcc tcgtcccatt cgatcggctc
     1381 gggcttgcgg acgagcgcga tacccagcac ctcgtccatc cttgcgacgg ggatgatctc
     1441 cagcgcattt ttcacgttat ccggaatttc agcgaggtcc ttcgcgttct cttccggaat
     1501 gatcacgcgc ttgatgccac cacgaagcgc agccaacagc ttctccttga ggccgccgat
     1561 aggcagcact cgcccgcgaa gtgtgatctc gccggtcatg gcaacatcgc ggcggactgg
     1621 aatgcccgtc agaacggaaa tgatcgccgt caccatggcc acgcccgcgg aaggcccatc
     1681 tttcggtgtc gcgccctccg gcacgtgcac gtgaatgtcc ttcttctcga acatcggcgg
     1741 ttcgattccg aaggccaccg cccggctgcg gacgtaagcg ttggccgcct ggatcgactc
     1801 cttcatcaca tccttgaggt tgccggtgac gttcatccgg ccgcggcccg gcatcatcac
     1861 gccttccaca gtcagaagct cgccgccaac ctcggtccaa gccaagccgg tgacaacgcc
     1921 gacctgatcc tcgagctcag cctcgccgta acgatgcttt tgcacgccaa ggaagtcgcg
     1981 aacggtttcg ccagtgacat gcacttgcgt ctctttgccg ctctcgatca gcttcactgc
     2041 cttgcgagcg agttttgcga tctcgcgttc caggctacgc actccggcct cgcgggtgta
     2101 acgccgtatg atctcgacga tg
//
LOCUS       DATMGX010000837         2184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_64250, whole genome shotgun sequence.
ACCESSION   DATMGX010000837 DATMGX010000000
VERSION     DATMGX010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64250"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VNR65_13710"
     CDS             <1..556
                     /locus_tag="VNR65_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19775.1"
                     /translation="FEYHVFPDQDCIQFTKKKDVVTIISGQQGWEITYRGKKDLPKEQ
                     LESALRRRDHSVEVAVKVWLKDPRTILFYDGQRTVQRHLADKVRIVNSENDSITIETD
                     AESHLPVRRTFQWRDPIYKDKNETSEEYDDYHAIDGFPTPFTITDYENGEMVGQRFLF
                     KANYNMSLPPDIFNPDATAAKIKK"
     gene            666..1175
                     /gene="fabZ"
                     /locus_tag="VNR65_13715"
     CDS             666..1175
                     /gene="fabZ"
                     /locus_tag="VNR65_13715"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HWJ19776.1"
                     /translation="MDPQTATPETSQLDIAAIMSILPHRFPFLLIDRVVEVVRRERIA
                     AIKNVTINEPFFQGHFPGYPVMPGVLVIEAMAQAGACLLLTEFPDRDEHLAFFTGIER
                     AKFRRPVVPGDQIRFEVKVLNWRSKACRMEGTAKVDGKLACEAVIMCQLVPRHRQSAA
                     SPTPAASTE"
     gene            1172..1948
                     /gene="lpxA"
                     /locus_tag="VNR65_13720"
     CDS             1172..1948
                     /gene="lpxA"
                     /locus_tag="VNR65_13720"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWJ19777.1"
                     /translation="MSVHATAIVAPSAVVPSSCTVGPYCTIGPNVALGEECDLISHVV
                     LDGHTTIGARNKFYPFASVGVAPQDISYAGEPTQLEMGDDNVIRESVTISRGTVKGGG
                     ITRIGSDCLFMTCAHIGHDSQVGSHCLFANAATLAGHVVVEDSASVGAFSAVHQFCYV
                     GRYAFIGGGTICTQDVLPYSLTAAKREARAYGINKIGLQRRGFSPERLKALQHAYKTL
                     LASRRNTTQAIAQLRSEGTSTDDVAYLIECVERSRRGVIK"
     gene            1951..>2184
                     /locus_tag="VNR65_13725"
     CDS             1951..>2184
                     /locus_tag="VNR65_13725"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19778.1"
                     /translation="MKLGLIAGNGRFPFLLLEAARAHGDEVIVAAIKEETDPEMDAVA
                     ARDQGVTVFWVSLGELSRLIETFRARGVTRAVMA"
BASE COUNT          454 a          704 c          599 g          427 t
ORIGIN      
        1 ttttgaatat cacgtcttcc ctgaccagga ttgcatccag ttcaccaaga agaaggacgt
       61 cgtcaccatc atctctggcc agcagggctg ggagattacc taccgcggaa aaaaagatct
      121 gcctaaggag caattggaga gcgccctgcg gcgccgcgac cattccgttg aagtggcagt
      181 caaggtgtgg ctgaaagatc cccggactat cctcttttac gacggacagc gcaccgttca
      241 gcggcatctc gccgacaagg tacgcatcgt gaactccgaa aatgactcca tcacgatcga
      301 gacggatgcg gagagccatc ttcccgttcg ccgtaccttc cagtggcgtg atcccatcta
      361 caaggacaag aacgaaacct ccgaagagta tgacgattac cacgccatcg acggctttcc
      421 tacacccttc accatcaccg actacgagaa cggcgagatg gtcggccagc ggttcctgtt
      481 caaggccaac tacaacatga gtctgccgcc ggacatattc aatccggacg caaccgcggc
      541 gaagataaag aagtagccgc cggattgtga ctgcatctct atgtcgcgca agcaccttta
      601 cctgctgcac gtaactcatc attcagatcg acgcgcgtaa attgtataat cggcgctgca
      661 aacctatgga cccgcaaacc gcaactcccg agacctccca gttagacata gccgccatca
      721 tgtcgattct gccgcaccgc ttcccgtttc tgctcatcga ccgcgtggtt gaggtagtcc
      781 gcagggaacg catcgcggcg atcaaaaacg tcaccatcaa tgagcctttt tttcagggcc
      841 actttcccgg ctacccggtc atgcccggag tgctcgtcat tgaggcgatg gctcaggcag
      901 gtgcgtgcct gctgcttacc gagtttcccg atcgtgacga acacctcgcc ttcttcaccg
      961 gcattgaacg cgcgaagttt cgccgcccgg tagtgcccgg cgatcagatt cgcttcgagg
     1021 tcaaggttct caactggcgc tcgaaggcct gccgcatgga gggcaccgcc aaggtggatg
     1081 gcaagctcgc atgcgaggct gtcatcatgt gccagctagt tccgcgccat aggcagtcgg
     1141 cggcatcccc aactcctgcg gcgagcactg agtgagcgtt cacgccactg ccatcgttgc
     1201 tccctcggcg gttgtcccgt cgagctgcac ggtcggcccc tactgcacca tcgggccaaa
     1261 cgtagctctc ggcgaggaat gtgatcttat ttcccacgtg gtgctcgacg ggcacaccac
     1321 cattggcgcg cgaaacaagt tctatccctt cgcctccgtt ggggttgcgc cgcaagatat
     1381 cagctacgcc ggtgagccca cccaactgga aatgggcgac gacaacgtca tccgcgaaag
     1441 cgtaaccatc tcccggggca ccgtaaaggg cggaggtatt acgcgcatcg gcagcgactg
     1501 tctcttcatg acctgcgccc atatcggcca cgacagtcag gtcggaagcc attgcctctt
     1561 cgcgaatgcg gccacgctcg ctgggcacgt ggttgtggag gactccgcct ctgtcggagc
     1621 cttctccgcg gttcatcaat tctgctacgt gggtcgctat gctttcatcg gcgggggcac
     1681 catctgcacc caggacgtgc tgccctactc cctgaccgcc gccaagcgtg aagcgcgcgc
     1741 ctacggcatc aacaagatcg gcttgcagcg ccgcggcttt tcgccggagc gcctcaaggc
     1801 cctgcagcat gcctacaaga cactgctcgc ctcgcggcga aacactacgc aagctattgc
     1861 gcaactacgc tcagaaggca cctccaccga cgatgtggcc tacctgatcg aatgcgtcga
     1921 gcgctcccgc cgcggagtca tcaagtaaca atgaaacttg gcctcatagc cggcaatgga
     1981 cgcttcccct tcctgctgct cgaggccgcg cgcgctcacg gagacgaagt catagtcgcc
     2041 gccatcaagg aagagaccga tcctgagatg gacgccgttg cggcccgcga tcagggcgtg
     2101 acggtcttct gggtatcgct cggcgagctg tccagactga ttgagacctt ccgggcgaga
     2161 ggcgtgaccc gcgccgtcat ggcc
//
LOCUS       DATMGX010000838         2345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_35757, whole genome shotgun sequence.
ACCESSION   DATMGX010000838 DATMGX010000000
VERSION     DATMGX010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35757"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VNR65_13730"
     CDS             complement(<1..314)
                     /locus_tag="VNR65_13730"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWJ19779.1"
                     /translation="MDTLVPRDERDTVEIVTAAIANATPLEVSGAGTKAGLGRPVTAQ
                     LRVSTGGMSGVSLYEPSELVLSAGVGTPIREVTDLLAQRGQELAFEPMDYGALYGVEA
                     GSG"
     gene            complement(319..1800)
                     /locus_tag="VNR65_13735"
                     /pseudo
     CDS             complement(319..1800)
                     /locus_tag="VNR65_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
     gene            complement(1939..>2345)
                     /locus_tag="VNR65_13740"
     CDS             complement(1939..>2345)
                     /locus_tag="VNR65_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HWJ19780.1"
                     /translation="AAIARQVANRGQPCETPCALISGGETTVTVRGNGRGGRNAEFLL
                     SLGLALNGQAGIAALAADTDGIDGSEDNAGAFLLPDTPARAAALGIDLKAHLANNDAY
                     TVFEKLGDLLITGPTLTNVNDFRVILIDDRSL"
BASE COUNT          458 a          743 c          677 g          467 t
ORIGIN      
        1 ccggaaccgg cttctacgcc gtacagcgcg ccgtaatcca taggctcgaa ggcaagttcc
       61 tgcccgcgct gcgccaggag atcggtaact tcgcgaattg gcgtgcctac ccccgccgag
      121 aggacgagtt ccgacggctc ataaagcgat acgccgctca tcccgcccgt gctgacacgc
      181 aactgcgccg tgacgggccg tccgagtccg gccttcgtcc ctgctccgct aacctccaag
      241 ggagtcgcgt tggcaatggc agccgtcacg atttcaacgg tgtcgcgctc atctcttgga
      301 acgagagtgt ccatcggctc aaaacctcgg cagatgggga aagggcaact tgcccctgtg
      361 aacgtgcatc tttccgagtt ccgcacagcg tctgagaatg ggaaacactt tgcctgggtt
      421 cagccggtga tgggggtcga aggcgcattt cacccgctgc tgctgttcga gatcgatctc
      481 gttgaacatc gtgggcatga gatcgcgctt ctcgacgcca acgccatgct cgccggtaag
      541 gacaccgcca acctcgacac agagcctgag gatggcggcg ccgaaggctt cggccctctc
      601 cgtctcgccg ggggcgtttc cgtcgtagag gatgagcggg tggagatttc cgtcgccggc
      661 atggaacacg ttggcgacgc gcaggccaaa ttcgcgcgac atgtcctgca tctttgccag
      721 cacgaagccg agtttgttgc gcgggattgt gccgtccatg cagaggtaat ccggaccgat
      781 ggcagcgacg gccgggaacg cgtttttgcg tcctgcccaa aattgcagcc gctcgtccta
      841 gctctgcgag agtctaagga aggtggcgcc ctcgctgcgc gcgatgtccg atacgcgatc
      901 gatcagatag tcgacctcgg cgggcgtgcc atccacttcg cagatgatga tcgactccgc
      961 atcgcgcggg tatccgcaag gagaaaagtc ctcggcggcg acaatggcca tgcggtccat
     1021 catttcgagg ccgcccggta ttattccgtg ggcgatgatc gcagccacgc agtcggcccc
     1081 ggcttcgaca ctcggaaacc cgattagaat gccgcgggcg cactggggct ttcgcaggat
     1141 acgcaccgtg acctcggtga tgacgccaag caaaccctcg gaccccgtca ttaggcccat
     1201 gagatcgtaa aactcggaat cgagatgctt gccgccaagc cgcacgacct cgccgcccat
     1261 caatacgatt tcgagaccaa gaatattgtt ggtcgtcagg ccgtatttca gacagtgcac
     1321 accgcctgaa ttttctccga cattgccgcc aatagagcag gcgatctgcg atgacgggtc
     1381 aggcgcgtaa taaaagcctt cgtcctctac agccttcgtg atcgcgagat ttgtcacgcc
     1441 gggctgcaca cgcacgcagc ggttcggaaa atcaacttca agaatggaat tgaactttcc
     1501 cattccaagc aaaatgccgt cggcgagcgg cagcgctccg cccgacagcg atgtgcccgc
     1561 tcctcgcgga accaccttca cgttcatctc ggcggcaagc gccatgatgc tcgagacctg
     1621 ttcgacggtc tccggcagga cgacgatcag cggcagttgc cggtacatcg tgaaggcgtc
     1681 gcactcataa gcgcgaagct cggtttcctg gtctatgaca ttctcggatg gcacgatccg
     1741 acgcagcgcg gctgccaacg ccttccggtt gccgaggatc gcttcctcgg gcttcggcat
     1801 caggagcgcc acaacgcacc cccgccaccc gacagcggct ggctggccat ttccgtttcc
     1861 tcctcctcga accggcgggc ttttttttcc cttgccggaa ttctgcttat taaaccatcg
     1921 cttgaactcg cgcggaaatc acaaagacct gtcgtcgatc aagatgactc tgaagtcgtt
     1981 tacgttcgtc agggtcgggc cagtaatcag gagatcgcca agcttctcga aaacggtgta
     2041 ggcatcgtta ttggcaagat gcgccttgag atcgatcccg agcgccgctg ctcgcgctgg
     2101 cgtatccggc aagaggaacg cgcctgcatt gtcctcgctg ccatcgatgc cgtccgtgtc
     2161 tgcagcgaga gcagcgatcc ccgcctgccc gttaagcgcc agaccgaggc ttaagaggaa
     2221 ctcggcgtta cgcccgccac gtccgtttcc acggaccgta acggtcgttt ccccgcccga
     2281 tatcaaagcg cacggggttt cacacggctg tccgcgattt gcgacctgtc tggctatcgc
     2341 agcat
//
LOCUS       DATMGX010000839         2929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_8297,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000839 DATMGX010000000
VERSION     DATMGX010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8297"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2729)
                     /locus_tag="VNR65_13745"
     CDS             complement(<1..2729)
                     /locus_tag="VNR65_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19781.1"
                     /translation="MANNHYRGVAAEITDRVASTVVEAPPHDAELAEHAQEWAHTLVW
                     LPAVGKSTHFKERLKALSAKLDAVLAAVEVRTSSQTSLPEDVQWLDDNSRLVRSVQRE
                     LHEATNPLRRVPHVRTPSQIIMPRVIAVAQDLLKSVEYRYSDRAFATYMEAFQTVTPL
                     EMRELSLIVPALKLVLLEELAGRAEKALASAGSPQQRSELIASLRDLTEAPWKELLER
                     LIVFERILAADPAGAYSRMDFPSRELYRHTVAHLAEHSNCSEPEIAQLALDLARDSKN
                     HNVPDSRLALRKSHVGCYLIGEGSDELRRRAGVRLPFGERAQAFLRRHPEEFYLGGIE
                     MLTLLIVISIMSPVYNAFNTSYGRILAILLLLLPCSQSAVEVMNYLTTALLHPRILPK
                     LDFCEGIPNDCVTAVVVPTLLLNEKQVHHLVDDLEVRYLGNTSPNLHFALLTDLPDSA
                     ETPNHDDPLVDLCQELIRGLNEKYSANGFSAFAMFHRHRVYNPREGVWMGWERKRGKL
                     LDFNRLIKGEFDSFPVKVGDLSLLQRVRFVLTLDTDTELPRGTAHRLVGALAHPLNQA
                     IIDPRANMVTAGYGILQPRVGIGVTSAARSRLASIYSGQTGFDIYTRAISDVYQDLNG
                     EGTFTGKGIYEVQTLNQVLEHRFPRNALLSHDLIEGAYARAGLVSDVELIDEYPSHYS
                     AYNRRKHRWLRGDWQIVSWLFGRVPDEKGRRVPNPISFLSRWKIFDNLRRSLVEPGMF
                     LLLILGWTVLPGRPIYWTLVTIAVLFVPPWFEFVFSVTRALMSRRLEPVREAFIGLGT
                     AMVSEFLTLTFLAHQTLLSADAVLRTFYRRIVSRQRLLQWETAAEAEMGTGELAFVDV
                     LLNWTPVVALIVSAIVYFSHRHSFAAALPVLVLWAFSKPVSSWLNCPPRRLQKA"
BASE COUNT          673 a          863 c          853 g          540 t
ORIGIN      
        1 gccttctgca accttctggg tggacaattc aaccatgagg agactggctt gctgaaggcc
       61 caaagaacga gcaccggtaa cgccgctgcg aaggaatggc gatgggagaa atacacgatc
      121 gcactaacga tcaaggccac gacgggcgtc cagttcagca ggacatccac gaaagcgagc
      181 tcgccagtcc ccatctcagc ctcggccgct gtctcccatt gcagcaggcg ctgccgcgac
      241 acgatgcggc gataaaaggt gcgcaatacc gcatcggcag acagcaacgt ttgatgggcc
      301 aggaatgtga gcgtgagaaa ctcgctcacc atggcggtac caagcccaat gaaagcctcg
      361 cgcaccggct ccagccggcg agacatcagc gcacgcgtga cagagaatac aaactcaaac
      421 cacggcggca cgaataggac cgcaatcgtg accagcgtcc aatagatggg ccggcccgga
      481 agcacagtcc atcccagaat aagcagaaga aacattcccg gttccaccag gctgcggcgc
      541 aggttgtcaa aaatcttcca gcgcgagagg aaggaaatgg gattgggaac gcgacggccc
      601 ttctcatccg gtacccgccc gaataaccag ctcacgattt gccaatcgcc gcgcagccag
      661 cgatgcttgc ggcgattgta ggcgctgtag tgcgacgggt actcgtcgat gagctcgacg
      721 tcgctgacta atccggcgcg ggcgtaggcg ccctcgatca gatcatggct gagcagcgcg
      781 ttgcgcggga agcgatgctc cagcacctgg ttcaaggtct gaacttcgta gatgcctttc
      841 ccggtaaagg taccttcgcc gttgaggtcc tggtaaacat ctgagatggc gcgggtgtag
      901 atgtcgaacc cggtctggcc ggagtaaatg cttgccagcc gcgaccgcgc cgcgctcgtc
      961 acgccgatgc caacgcgtgg ctgcaaaatc ccatagccgg ccgtaaccat gttcgcccga
     1021 ggatcgataa tggcctggtt caaagggtgg gccagcgcgc cgaccaggcg atgagcggtg
     1081 ccgcgcggca gttcggtgtc cgtgtccaag gtcagaacaa agcgcacacg ctggagaagc
     1141 gaaagatcgc caaccttgac tggaaaactg tcgaactctc cctttattag gcgattgaaa
     1201 tcgagcagct tgccccgctt gcgctcccag cccatccaga ctccttcacg cgggttgtaa
     1261 acccggtgcc gatggaacat cgcgaaggcg ctaaatccat tggcagaata cttttcgttc
     1321 agcccccgaa ttaattcctg gcaaagatcc accaggggat catcgtggtt gggcgtctcc
     1381 gcagaatcgg gcaagtccgt cagcaaggca aaatgcaagt tggggctggt gttgccgaga
     1441 tagcgtactt ccagatcgtc caccaggtga tggacctgct tctcattgag tagaagcgtg
     1501 gggaccacga ccgcggtcac gcaatcgttg ggaatgccct cgcagaagtc cagcttggga
     1561 agaattcgag gatgcagcaa ggcggtcgtg agatagttca tcacttcaac cgcgctttgg
     1621 ctgcacggca acagaagcaa gagaattgcg agaatccgtc cgtaagacgt gttgaaagcg
     1681 ttataaaccg ggctcatgat cgagataacg atcaacagcg tgagcatttc aatcccgccg
     1741 aggtaaaact cctcgggatg gcggcgcaga aacgcctgcg cgcgttctcc gaacggaagc
     1801 cgcacaccag cccgccgccg aagttcatct gaaccttctc cgatcaggta acagccgaca
     1861 tgggacttgc gcagcgccag acgcgaatcc ggtacattat ggttcttgga atcgcgggcg
     1921 aggtccaagg caagctgcgc aatttccggt tccgaacaat tcgaatgttc ggcgagatgc
     1981 gccaccgtat ggcggtagag ctcccggctt ggaaaatcca tgcgcgagta agcgccggcg
     2041 ggatcggcag ccagtattcg ctcgaagacg atcaaccgct ccagtaactc tttccaaggg
     2101 gcttcggtta gatcgcgcag gctggcaatc aactcgcttc tctgttgcgg cgaacctgcg
     2161 ctcgccagcg ccttctcagc acgccccgcg agttcttcga gcaatacgag tttcagagcg
     2221 ggcacgatca gagaaagctc acgcatttcg agaggtgtca ccgtctggaa cgcttccatg
     2281 taggtagcga aggcgcggtc ggaatagcga tactcaaccg atttcagcaa atcctgggcg
     2341 accgcaatca cccgaggcat gatgatttgg ctgggagtcc gaacgtgagg aacccgccgc
     2401 aaaggattcg ttgcctcgtg caactccctt tgcacagagc gaaccagacg cgaattgtca
     2461 tccagccact ggacgtcttc cggaaggctg gtttgggagg aagttcggac ctccaccgcc
     2521 gccagaactg catcgagctt ggcgcttagg gccttgaggc gctccttgaa gtgagtggat
     2581 ttgccaactg cgggaagcca aaccagggtg tgcgcccact cctgagcgtg ctccgccaac
     2641 tccgcgtcgt gcggcggcgc ttcgaccacg gttgaagcca cccggtcagt gatttcagcc
     2701 gccacgcccc gataatggtt attggccatt gcgagttcca agcaaatttc tcagcgatag
     2761 ctcgcggcct gcatttcaaa cattgcagcg taggagccgc cgagcgccat caactgctca
     2821 tgactgccat cttcggcgat ctgtccatcg gccagcacca caattcggtc ggccatgcgc
     2881 acggttgaaa accggtgcga gatgaagagc gccatcttgc cccccgtca
//
LOCUS       DATMGX010000840         3495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_303309, whole genome shotgun sequence.
ACCESSION   DATMGX010000840 DATMGX010000000
VERSION     DATMGX010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303309"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VNR65_13750"
     CDS             <1..204
                     /locus_tag="VNR65_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19782.1"
                     /translation="HSHAITNVKENLLVELTHVSLGLLAVFAGWARWLEIRLPPKDRA
                     IPAWIWPVCFALIGVGLLNYREL"
     gene            complement(437..2641)
                     /locus_tag="VNR65_13755"
     CDS             complement(437..2641)
                     /locus_tag="VNR65_13755"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HWJ19783.1"
                     /translation="MVFKLPESREIKARLQRVLRPKSKLGRTTLWFGGLALVLELLRL
                     IFRAPNGTMLSGWASFLVFIFAVCATLMGVRWVRRQFMWRLRNRLIVTYVFIGVIPIF
                     LLMTMAILACYLFGGQFATYVAMSDLQSELQHLESANRSLAMQFRSLARSGKLNEQLA
                     AEIASASDENFRNRVVSVWDGDKPFVLAAGRPAPGVEPVRESAAIQGDFAGFVLDGKR
                     LHLRVVKHVEAGSHRLTVISNVPVTPQLLRMATSQLGSVTVIPPDEDKKTESDTPSSS
                     SPRAPKSSSVPDKDKLIIDMGDSHATVSLSGENASSRVEAGRLPPPANSFDKHIPFAT
                     RFSAIDWHTGKSLTGLILVDTRPSMLYATLFSALGDKAEIFLDVLVGIAIFFGVIELI
                     ALFIGVRISRSITKSVADLYVATQHVNRGDLAHRIKVRTHDQMAALEQSFNSMTQSLS
                     NLMSEQKEKQRLESELAIAHEVQDLLFPADLSGLATLEVHGVCRPARTVSGDYYDFIP
                     LHEDKLVLAVGDISGKGISAALLMATVHAFVRAYSLEPERTLASVALDAGLPRDSALA
                     LPMYYRGDGTSDSQLAPGLLMATLNYQLYRSTPPEKYATMFLGCYDAASRSLTYSNAG
                     HLPPLVLGHNGQVRRLDTSGTVVGLFDGMSYDESTIGMEPGDMFVAYSDGVTEPENEF
                     GEFGEERLIELIQRHRDQPLARISEVVTGAVTEWIAGGEQPDDVTLVLARAR"
     gene            complement(2758..2958)
                     /locus_tag="VNR65_13760"
     CDS             complement(2758..2958)
                     /locus_tag="VNR65_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19784.1"
                     /translation="MAKLTLVYGMRLLPSEEVAEVGEGAVKLKNGREAHVTLHIIEGS
                     KEEIERQLRTSVEAFFDLYPEI"
     gene            3123..>3495
                     /locus_tag="VNR65_13765"
     CDS             3123..>3495
                     /locus_tag="VNR65_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ19785.1"
                     /translation="MPDNVRNVIIIGSGCSGLTAAIYAARANLSPLVIDGHEPGGQLA
                     ITTLVENFPGFPDGIQGPELIENMRKQAARFGAEFLAGHVAAVDLSQKGPAGSKKPIE
                     VNLGRETLLTRTLIIASGASAR"
BASE COUNT          702 a         1017 c         1020 g          756 t
ORIGIN      
        1 cactcccatg ccattaccaa tgtgaaggaa aacctgctgg tggagttgac ccacgtatcc
       61 ctgggcttgt tggcggtgtt tgccggctgg gcgcgctggc tggaaattcg ccttccgccc
      121 aaagatcgcg ctattcccgc ctggatctgg ccggtctgct tcgcgctgat cggtgtcggt
      181 ttgctgaact accgcgagct gtaggctgcc cgcctaggaa ttgtcttgac gagcagctca
      241 tgggcctggg gcctgctctg tcaaaaatca tttaccacgg agacacggag gacacggagt
      301 ttttggtttc tatcgggttt tctccgtgat ctccgtgcct ccgtggttga ttttcatttc
      361 tatgattggg ccgtaggaca atgtgaacct cagggtgaca accaatgaac caagtcacag
      421 cgccgctgga actattctag cgtgcgcggg cgagcaccag ggtcacatcg tccggctgtt
      481 cgccgccggc tatccactcg gtaactgcgc cggtgaccac ctcgctgatg cgcgccagcg
      541 gttgatcgcg atgccgctgg atcagctcaa tcagacgctc ctcgccgaat tccccaaact
      601 cgttctcggg ctcggtgacg ccgtcgctgt aggcgacgaa catatctccc ggctccatgc
      661 cgatggtgct ttcgtcgtaa ctcatcccgt cgaatagacc tacaacggta ccggaggtgt
      721 ccagacgcct gacttggccg ttgtggccca ggaccaatgg aggaaggtgt ccggcgttgg
      781 aataagtgag cgagcgcgat gcggcatcgt agcagcccag gaacatggtc gcgtactttt
      841 ccggcggcgt gctgcgatag agctgatagt tcagggtggc catcaacaat cccggggcca
      901 gttgggaatc gctggtgcca tcgccgcgat agtacatggg gagagcgaga gcgctgtccc
      961 tgggaaggcc ggcgtccagc gccacggagg ccagagtccg ctccggctcc agcgaatagg
     1021 cgcggacgaa cgcgtgcact gtggccatca gcagcgccgc cgaaatacct ttgccgctga
     1081 tatcgcctac cgccagcacc agcttgtctt cgtgcaaggg gatgaaatcg taatagtcgc
     1141 cgctcacggt ccgcgccgga cggcacactc catgcacctc cagcgtggcc aggccggaca
     1201 aatcggcggg gaagagcaag tcttgcacct cgtgtgcaat cgccagttcg ctctccagcc
     1261 tctgcttctc tttttgctcg ctcatcaagt tcgacagcga ctgggtcatg gagttgaacg
     1321 attgctccag cgcggccatc tgatcgtggg tgcgcacctt gatgcgatgg gccaggtcgc
     1381 cgcgattcac gtgctgggtg gcaacgtaga gatcggccac ggatttggtg atgctgcggc
     1441 tgatgcgcac gccgatgaac agcgctatca gctcgatgac accaaaaaaa attgcgatgc
     1501 caaccagaac atcgaggaag atctcggctt tgtctccgag agcgctgaac aacgtggcgt
     1561 acagcatcga gggccgagta tccaccagta tcagcccggt cagggatttt ccggtatgcc
     1621 aatcgatcgc gctgaatcga gtggcgaatg gaatgtgctt atcgaagctg tttgcgggag
     1681 gtggcaggcg tccggcctcc acccttgagc ttgcgttttc acccgacagg cttaccgtcg
     1741 cgtgtgaatc tcccatgtcg atgatcagct tatctttatc gggtactgaa gaggatttag
     1801 gagcgcgagg ggagctactc gatggagtat ccgattctgt ttttttgtcc tcgtctggag
     1861 gaatcacagt taccgatcct aactgcgacg tcgccatccg cagcagctgc ggagtgacgg
     1921 gcacattgga gatcactgtc aggcgatgag aaccggcttc cacgtgtttg accacgcgaa
     1981 ggtgcagacg cttgccgtcg agcacaaatc cggcgaaatc tccctgaatt gccgcggatt
     2041 cacggaccgg ttcgacgcct ggagctggtc tgccagccgc caggacgaaa ggcttgtctc
     2101 cgtcccagac gcttaccacg cgattgcgga aattctcatc ggaggcgctg gcgatctcgg
     2161 cggccagttg ctcgttcaat ttcccggagc gcgccaagct gcggaattgc atggccaggg
     2221 accggttggc tgattccagg tgctgcaatt ccgattgcag gtccgacatg gcgacatagg
     2281 tcgcaaattg tcccccaaag aggtagcaag cgagaattgc catcgtcatc agcaggaaga
     2341 tgggaatgac gccgatgaag acgtaggtga cgattaagcg gttacgcagg cgccacatga
     2401 actgccgacg cacccagcgt acgcccatca gcgtggcgca gacagcaaag ataaagacga
     2461 gaaagctggc ccagccgctc agcattgtcc cgttaggcgc acggaagatc aagcgcagca
     2521 gttccaggac cagggccaat ccgccaaacc acaacgtggt tcgccccaac ttgcttttgg
     2581 ggcgaaggac cctttgcagc cgggccttga tttctctgga ctctggaagc ttgaacacca
     2641 taagctcttg gaattattgc ttttcggtgg cgttcgccgc cagcattaag tttttacggt
     2701 tatgcgccgg gaaacgtttc aatgtttgcg aggccgtacc gctcccgaca agctttctca
     2761 aatctcggga tagagatcga aaaacgcctc cacgctcgtt cgcaactgcc gctcgatctc
     2821 ctctttcgag ccttcgataa tgtgcagcgt cacgtgcgcc tcgcgcccat tcttcagctt
     2881 taccgcaccc tcgcctactt cggcgacttc ttctgacggc agaaggcgca ttccatacac
     2941 cagggtcaac tttgccatgt cctgattcta aacgttccac aaagctcagg cacttgttag
     3001 aatccggacg gtacattctg ttcgttcgag ctttcgagta gcaagccatt atttcccttg
     3061 tgcgcctcgc ggacgggtcg ggttgcatct tccggcgcgg cgcggaatct aagtccagtc
     3121 ctatgccaga caatgtgcgt aacgtcatca tcatcggttc cggatgttcg ggactgaccg
     3181 cagcgatcta tgctgcccgc gccaatctta gcccgcttgt catcgatggg catgagcccg
     3241 gcgggcagct tgccatcacc acgctggtgg agaattttcc gggatttcca gacggcatcc
     3301 agggaccgga actcatcgag aacatgcgta agcaggccgc gcgttttggc gccgagtttc
     3361 tcgctggaca tgtggctgcg gtggacctca gccaaaaggg ccctgccggc agcaagaaac
     3421 cgatcgaggt gaatctaggg cgcgagaccc tattgacccg tactctcatc attgcgagcg
     3481 gcgcgtcggc gcgct
//
LOCUS       DATMGX010000841         2490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_119835, whole genome shotgun sequence.
ACCESSION   DATMGX010000841 DATMGX010000000
VERSION     DATMGX010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119835"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1121
                     /locus_tag="VNR65_13770"
     CDS             318..1121
                     /locus_tag="VNR65_13770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ19786.1"
                     /translation="MSASNPFFAPKTALITGASSGIGLELAHLFARDGYRLVLVARSR
                     GALRELGDDLQSRYSVTVRISPKDLAHPATPNELYQELQEAGIVLDVLVNNAGFGGGG
                     PFLSTEWTNEAEMMQVNMVALTQLTKLFLPQIRAREGEILNVASTAAFQPGPFMAVYY
                     ASKAYVLHFTEALAEELRGTGVTVTCLCPGPVKTNFQKRAHIGDTRLMTSPLLVDVRE
                     VARVAYEGMKQGKRVVIPGWKNRFGVQMLRLSPREMVTKVVRKIQESKK"
     gene            complement(1203..1973)
                     /locus_tag="VNR65_13775"
     CDS             complement(1203..1973)
                     /locus_tag="VNR65_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HWJ19787.1"
                     /translation="MSLSAVIVDDEQLARDELAYLLKELDVDVVAQGKNGMDAVNLIK
                     EFSPELVFLDVQMPGLDGFGAIKKLIDKKVPLPQIVFATAYDQYAVKAFEVNAVDYLL
                     KPFDKKRVAKAVEKARQKLETAGSPGEQIGTIVKMLEQQARPPAAKVLLKSGGRLFLV
                     DQKDICYASIEDGIITVVATGMEGQSNCRTLEELLDSLDPNMFWRAHRSYLVNINRIK
                     EVVPWFKSSYQIRMEDRKQTEVPVSRAQTKRLRELFGL"
     gene            2236..>2490
                     /locus_tag="VNR65_13780"
     CDS             2236..>2490
                     /locus_tag="VNR65_13780"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HWJ19788.1"
                     /translation="MVVIYGIHSVSEALKSGARSFEYVGIARERHDQRVQRLIDDCRA
                     SGVQVRFLPREQLDRVANTNTHQGVIAVTSSKQYADVDTLL"
BASE COUNT          522 a          731 c          722 g          515 t
ORIGIN      
        1 gcaaagcccg gagagatatt ctggttcatc accaagggaa agctggaatc gggcatgcct
       61 tcgtgggcta gcctgcctca acagcagcgc tggcagatcg tgactttcct ggagtcgcag
      121 ggcaacgcga aggccgaagc gaagtgagcg tccgcttcgt cgaaaatcat tcaccacaga
      181 ggacacggag gttccggttt ctgtcgagtt ttctccgtga cctccgtgat ctccgtggtt
      241 gattttcatt gtatgcagct cgggatttgc ccttcttgcc actgctgcga tgacccgaaa
      301 tccgctataa cagagttgtg agtgcaagca atcccttctt cgcccccaag acagccctga
      361 tcacaggcgc gtccagcggc attggactgg aactggcgca cctgtttgcg cgcgacggct
      421 atcgtttagt gctggtggcc cgcagtcgcg gcgcgctgcg tgaactcggc gacgacctgc
      481 aatcgcgcta cagcgtgacg gtacgtatct cgcccaagga cctggcacat ccggctacgc
      541 cgaatgagct ctaccaggaa ttgcaggaag ctggcatcgt cctcgacgtg ttggtgaaca
      601 atgccggctt cggcggcggc ggaccgtttc tttccaccga atggaccaac gaagcagaga
      661 tgatgcaggt gaacatggtg gcgttgaccc agctcaccaa gttgtttctg ccgcagattc
      721 gcgcgcgcga gggggaaatc ctcaatgtcg ccagcaccgc cgcgtttcaa cctgggccat
      781 tcatggccgt gtattacgcc agcaaggctt acgtactgca tttcaccgaa gcgctcgcgg
      841 aagagttgcg tggcaccggc gttaccgtga cttgcctctg tcccggaccg gtgaagacca
      901 acttccagaa acgcgcccac atcggcgaca cgcgtttaat gaccagccct ctgctggtgg
      961 acgtgcgcga ggtggcgcgt gtggcgtatg aaggcatgaa acagggcaaa cgcgtggtca
     1021 ttccgggatg gaagaaccgg tttggcgtgc agatgctgcg cttgtccccg cgcgagatgg
     1081 tgaccaaggt ggtgcggaag attcaggaga gcaagaagta gcaggcagca actagcaatt
     1141 agcaactagc aaaacagccc agcccgcgaa aattgctagc tgctagctgc tagctgctgc
     1201 tcttacagcc caaacagctc ccgcaggcgt ttggtctggg cgcgactgac agggacctca
     1261 gtctgttttc tgtcttccat gcggatctgg tacgagctct tgaaccaggg cacgacttcc
     1321 ttgattcggt tgatattcac cagatacgag cgatgcgcac gccagaacat gttgggatcg
     1381 agcgagtcga gcagctcctc tagcgtgcgg cagttcgact ggccttccat cccggtcgcc
     1441 acaacggtga tgatcccgtc ctcaatcgag gcgtagcaaa tgtctttctg gtccaccaga
     1501 aaaagcctgc cgcccgactt gagcagcact ttcgcggctg gcggacgcgc ctgctgctcc
     1561 agcatcttga cgatggtccc gatttgctcg ccgggcgagc cggcggtctc cagcttttgc
     1621 cgggccttct ccacggcctt cgcgacccgc ttcttgtcga acggcttcag caggtagtca
     1681 accgcgttca cctcgaacgc cttgacggca tactgatcgt acgcggtggc gaatacgatt
     1741 tgcggcagcg gcactttctt gtcgatcagc tttttgatgg cgccgaagcc gtcaagtccg
     1801 ggcatttgca cgtcaaggaa gacgagttcg ggcgagaact ccttgattaa gttcactgcg
     1861 tccatgccgt tcttgccttg cgccacgaca tccacatcaa gctctttcaa cagataggcg
     1921 agttcgtcgc gcgcgagttg ctcgtcatcg acaatgacag cggatagtga catccaatct
     1981 cctcaatcca gaactgctca agcataacta tacccagatg gagcgccgga accaaccggc
     2041 gaaggaaatc gggctactcg gaacaactca catgcggcat cctcaaagag gatagcggtt
     2101 cagtcgaaag caggtccctc cgctcgggat gacaacatca gggaattcga cagctctggc
     2161 tgacagctga aagctgacgg ctgatagctg acggctgaca gctgacagct cccgtgtcgt
     2221 acactaattt caaacatggt cgtcatctac ggtatccatt cggttagcga ggcgctgaag
     2281 tccggggcgc gcagctttga atacgtcggc attgcgcgcg agcggcatga ccagcgggtg
     2341 cagcgcctga ttgacgactg ccgcgccagc ggcgtgcagg tgcgcttcct gccgcgcgag
     2401 caactcgatc gcgtcgccaa caccaatacc catcagggcg tcattgctgt taccagcagc
     2461 aagcaatacg cggacgtgga cacgctgctg
//
LOCUS       DATMGX010000842         2165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_49334, whole genome shotgun sequence.
ACCESSION   DATMGX010000842 DATMGX010000000
VERSION     DATMGX010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49334"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VNR65_13785"
     CDS             complement(<1..1226)
                     /locus_tag="VNR65_13785"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ19789.1"
                     /translation="MKVIIVGGVAGGASCAARLRRLDEKADIMMVERGPYISYANCGL
                     PYHVGGVIEKESSLLVASEQLFRERFAIDVRTGCEAIGISPKEKKVKLRSVKTGEVTE
                     HTYDKLVLSPGAVSIRPPLPGIDLPGIFQVRTVPDARQIKEWIRERRNGERQMRAVVV
                     GGGFIGLEMADNLVHLGIEVTLVEMATQVLPPVDEEFARIVEARLEREGVRLALGDGV
                     AAFEQAQNGGIDVRTKSGKAHTGDLVILALGVRPDTALAKAAGLQIGERGGIRVDDQM
                     RSSDPDIFAVGDAIEVKDFVTGESSLIALAGPANRQGRIAADVIAGRNSRFRGTQGTA
                     IVGLFGAAIAWTGVSEKALKRLGDSDYEKIYLYPTSHAGYYPGAKPIAMKVLFRKSDG
                     RLLGAQAFGEDNVDKR"
     gene            complement(1509..1964)
                     /gene="trxC"
                     /locus_tag="VNR65_13790"
     CDS             complement(1509..1964)
                     /gene="trxC"
                     /locus_tag="VNR65_13790"
                     /inference="COORDINATES: protein motif:HMM:NF008229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HWJ19790.1"
                     /translation="MNTIETIACTSCGTVNRAPASKLAAGARPTCGSCHRPLFTGEPY
                     EVATDEEFDRFLSQTSLPVLVDFWAPWCSPCLQMAPHFKAAAQTLEPRLKLLKADTEN
                     LPGAAVRHGIRSIPTMILFANGSEIARQSGAMPAKGIEQWVKQQLPGGR"
BASE COUNT          423 a          687 c          631 g          424 t
ORIGIN      
        1 atgcgcttgt cgacattgtc ttcgccaaag gcttgtgctc caaggagacg cccatcggat
       61 tttcggaaca gcaccttcat cgcgatcggc ttggcgcccg gataatagcc agcgtgcgaa
      121 gtcgggtaga ggtagatttt ctcgtaatcg ctgtcgccga gccgcttcaa ggctttctcg
      181 ctgacgccgg tccaggcaat cgccgcgccg aaaagaccaa cgatggcggt tccctgcgtg
      241 cctctgaacc gcgaattgcg gcccgctata acatcggctg cgatgcgccc ctgtctgttt
      301 gcagggccgg ccagtgcaat gaggctcgac tcgccggtga cgaagtcctt gacctcaatg
      361 gcgtctccga cagcaaaaat atccggatcg ctcgaccgca tctgatcgtc gacgcggatg
      421 ccgccgcgct cgccgatttg aagcccagcc gctttggcga gagccgtatc ggggcgcacc
      481 ccgagtgcaa ggatcacgag gtcgcccgta tgggccttgc cggatttcgt tcgcacatca
      541 atcccgccgt tctgggcttg ctcgaatgcg gcgaccccat cgccgagcgc gagccggacg
      601 ccctcgcgtt ccaggcgcgc ctctactatg cgggcgaatt cctcatcaac gggaggcaga
      661 acctgggtgg ccatctctac aagggtgact tcaatgccca gatgaaccag gttatcggcc
      721 atctccagac cgatgaaccc gccgccgacg acaacggcgc gcatctgccg ctcaccgtta
      781 cggcgttctc taatccattc tttgatctgc cgggcgtcag gcacggtgcg aacctgaaag
      841 atcccaggca ggtctatccc cggcaaaggc ggacgaattg acaccgcccc tggcgacaga
      901 accagcttgt cgtaggtgtg ctcggtcacc tcgccggtct tcacgctgcg cagcttgacc
      961 tttttctcct tcggcgaaat ccctatggcc tcgcagccgg tccggacatc aatggcgaac
     1021 cgctcgcgaa acagctgctc gctggcaacc aggaggctcg attccttctc gatgacgcca
     1081 ccgacgtgat aaggcagccc gcaattcgcg tacgatatgt aaggcccccg ctccaccatc
     1141 atgatgtcgg ctttctcgtc gagcctgcgc aggcgcgcgg cgcaggaggc tccgccagcc
     1201 actccaccga caatgatgac tttcatgggc ttctccctct tatcgtgaat ctcaaatgcc
     1261 tcggggatcg cgaaacgaaa acctgagggg cggatgaaga gcgcgaaacg accgcggcag
     1321 ggcctataat tgcgagccaa tctacacccg cagtggcgat aatttcctgg cgctccttcc
     1381 tgccatcttg cagcaaggaa cagctccttg aggcagtgag agaaaagccg ctcttggaag
     1441 aggtgtctgt aactaagtca cagacgctca atgcctgatg cgctgccggg caatagcggc
     1501 acgacaactc agcgtccgcc aggaagctgc tgtttgaccc attgctcgat ccccttcgcc
     1561 ggcatggcgc cgctctgccg cgcgatctcg ctcccgttcg caaacaggat catcgtcgga
     1621 atgcttctga tgccgtggcg gacggccgcg ccgggaagat tttccgtatc ggccttcaag
     1681 agcttgaggc gaggttcaag cgtctgcgcc gcggccttaa aatgaggcgc catctgcagg
     1741 caagggctgc accagggcgc ccagaagtcg acgaggacag gcaacgaagt ttgagacaga
     1801 aacctgtcga actcttcgtc cgttgcaacc tcgtacggct cgccggtaaa gagcggccgg
     1861 tggcagctgc cgcatgttgg gcgggcgccg gcggcaagct tcgaggcagg ggctctgttg
     1921 acagttccgc aggaggtgca agcaatcgtc tcaatcgtgt tcatttgctt ttgcctcatg
     1981 attcttgcgt catttcaccc ggaagacggc gtggcaggca ttgcaccgca ccgtaatctt
     2041 ggcgaggcca tccaccagct ttgaccagcc cgccgcatcg ggcggctccg ggacctcaga
     2101 ggcgatacgg gcaaagtcgt cggctgctcg atggtactca aaacccatct cgcggaactc
     2161 gggcg
//
LOCUS       DATMGX010000843         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_308041, whole genome shotgun sequence.
ACCESSION   DATMGX010000843 DATMGX010000000
VERSION     DATMGX010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308041"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..548)
                     /locus_tag="VNR65_13795"
     CDS             complement(78..548)
                     /locus_tag="VNR65_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWJ19791.1"
                     /translation="MTKENTTPVNITREQMIQLLNEDLAGEYQAIIAYTVYSQVLKGA
                     AYTDIARELELHAGEELAHAIKIAKQIDYLGGMPCVTPKPVKTSNDPVTMLRADLENE
                     RTTVGRYRERIRQAEAMGEFALSETLRAIIVQEQEHEIDLSAALDINVPLPKAT"
     gene            complement(661..1632)
                     /locus_tag="VNR65_13800"
     CDS             complement(661..1632)
                     /locus_tag="VNR65_13800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWJ19792.1"
                     /translation="MKSATAATVLASATPQVLLAETKKEMPQRLLGRTGEKVSAIGLG
                     GFHIGNQADEAESITIVRSAIDQGITFMDNCWDYNGGTSEIRMGKALRDGYRQKVFLM
                     TKCDGRTKAAATRQIDESLKRLQTDRIDLMQLHEIIRLEDPDRIFAAGGAIEAMVDAR
                     QAGKIRYIGFTGHKDPFVHLRMLEVAAAHKFRFDTVQMPLNVMDAHFRSFEHQVLPRL
                     IADEIGVLGMKSMGDPFILGSKTVTPIECLHYALNLPTSAVITGIDSLKILDQALEAA
                     RTFKPMSKETLNRLLARTATAAATGRYELFKTDSRFDGTAQNPQWLG"
     gene            complement(1863..>2098)
                     /locus_tag="VNR65_13805"
     CDS             complement(1863..>2098)
                     /locus_tag="VNR65_13805"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HWJ19793.1"
                     /translation="IAYVGGRDVLNAIEKSCPGFDLTPSRQVLRECGNMSSPSVLFAL
                     ERALRDGTPDETGDWWLTSFGAGFSAHGCRVGL"
BASE COUNT          413 a          588 c          574 g          523 t
ORIGIN      
        1 ttctgtttgg cagtatagct ggacttgtcg ccttgaggca tggttgtctc cttcctggtt
       61 tcaaatgttc acgggcccta agtcgccttg ggaagaggca cgttgatgtc cagcgcggcg
      121 ctcagatcaa tttcatgctc ctgctcctgc acgatgatgg cgcgcagggt ttcgcttaac
      181 gcaaattccc ccatcgcctc ggcctggcgg atgcgttcgc gataacgccc caccgtggtc
      241 cgctcgtttt ccagatcggc gcgcagcatg gtgaccggat cgttggaagt tttgactggc
      301 ttgggagtca cgcacggcat gccgccgaga taatcaattt gtttggcaat cttgatggca
      361 tgagccagtt cctcgccggc atgcaattcc aattcacgcg cgatgtccgt gtaagcggcg
      421 ccttttagca cctggctata caccgtgtaa gcaatgatgg cctgatattc tccggccaga
      481 tcctcgttca acaattggat catctgttcg cgggtgatgt ttactggggt cgtgttttct
      541 tttgtcataa tgttttaaaa attaatctgt ggtttctcac ttgatggcgt tcagtctttc
      601 acggcaaagc cgactttgat ggtcacctgc cagtgaagtt catgattatt gccaaagaac
      661 tcaacccagc cactgcggat tttgagccgt cccatcaaaa cggctgtcgg tcttaaagag
      721 ttcatacctg ccagttgcgg cagccgtcgc cgtgcgggcg aggagccggt tgagagtttc
      781 cttgctcatc ggcttgaacg tccgcgccgc ttcgagcgcc tgatcgagga tcttcaggct
      841 gtcaatgccc gtgatcaccg ctgaggtcgg caggttaagt gcataatgca aacattcaat
      901 cggcgtcacg gttttgctcc caagaataaa gggatccccc atagatttca ttcccagcac
      961 cccgatctcg tccgcaatta agcggggaag aacctgatgc tcgaaactcc gaaaatgcgc
     1021 atccattacg ttcaaaggca tctgcacggt gtcgaagcgg aatttgtgag cggcagcgac
     1081 ctccagcatc ctgagatgaa cgaacggatc cttatggccg gtaaaaccga tgtaacggat
     1141 cttccctgct tgcctggcgt ccaccatggc ttcgatcgcg ccgccggctg caaagattcg
     1201 gtccgggtct tctaatcgga tgatctcatg caactgcatc agatcaattc gatccgtctg
     1261 caaccgcttc agggattcat caatttgccg cgtggccgcc gccttggtgc ggccgtcgca
     1321 cttggtcatg agaaagactt tttggcggta gccgtcacgc aaagccttcc ccatgcgaat
     1381 ttcgctggtc ccgccattgt aatcccagca gttgtccatg aaggtgatcc cctggtcaat
     1441 ggcgctccga acaatggtta tgctctccgc ttcgtcagcc tggttgccga tatgaaagcc
     1501 acccaggccg atggctgaga ctttctcgcc cgtccgtccc agcaagcgct ggggcatttc
     1561 cttcttggtc tcggccagca aaacctgtgg cgttgcgctg gccaataccg tcgccgccgt
     1621 ggcggacttc aagaacgagc gtcggctcat gactgtgtca agttgacttg gcggatcgag
     1681 ctgggacatc ttgtcagaag ttgtgcgcat attttgtctt gggagaagtt atggtttttc
     1741 gtgctttgag aatagactgc aacctggggc gttcgctatg gggtgttcac cccagctcgt
     1801 ctccatggaa tcgagcggct caaagttggg atattcgcgg cggggcattt cgtccgtcac
     1861 catcaaagtc cgacgcggca gccgtgggca ctgaagccgg caccaaagct cgtcagccac
     1921 cagtcacctg tttcatcggg cgttccgtcg cgcaaggccc gttccaaggc gaacagcacc
     1981 gaagggctgc tcatgttgcc gcattcgcgc aatacctgtc ggctgggggt taggtcaaag
     2041 ccggggcagg atttttcaat cgcattgagc acatcgcgtc cgccgacgta cgcgatca
//
LOCUS       DATMGX010000844         2594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_81448, whole genome shotgun sequence.
ACCESSION   DATMGX010000844 DATMGX010000000
VERSION     DATMGX010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81448"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..1717
                     /locus_tag="VNR65_13810"
     CDS             476..1717
                     /locus_tag="VNR65_13810"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWJ19794.1"
                     /translation="MPSLALSDLIGAPVIDNTGTTAGKVREVTLVPQENSSQIAGFVV
                     KTRHGDRLLPTKSLSLINGGLRVATPASEWGPYAGGEGMLLLERDLLDQQIIDVHGRK
                     VVRVNDIDIHEELGHDHIVLKLGAVDVGARGAVRRLLKGVIPAAVLQLLLTKIPPRMI
                     PWEFVDLIEIDPARRVKLKISLDRLAKLHPADIADIVEDLAPAEREAIFETLDDGVAA
                     ETLEEIDPKMQVSILASLDSDRAADIVEEMDPDAAADLLGDMHPDDSQEILTEMQPEE
                     RAEVAGLMNFEEDTAAGRMTTDYMALGPDGKVEDAIEMLRNFEGGVESISTIYIVGDK
                     NKLLGAVPLAKIVLAPPGTLLMMLSTGHLTTVHPGADEKTVAELFDKYNLLTLPVLDE
                     DGKLTGVITADDVINMLRAKL"
     gene            1752..2042
                     /locus_tag="VNR65_13815"
     CDS             1752..2042
                     /locus_tag="VNR65_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19795.1"
                     /translation="MTQTVIRISEAEAESDFGSVLARVRAGAEVIIESESGGPPIAVV
                     RPVGSRPGRLLSESIALAEAHASTATLDADFSHDLEAIINSHREPLNPPAWD"
     gene            2033..2446
                     /locus_tag="VNR65_13820"
     CDS             2033..2446
                     /locus_tag="VNR65_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWJ19796.1"
                     /translation="MGLILDSSVAIAAERRGDNVTQLLDQIVAAAGNQEAVLSSIGLT
                     ELVHGIYRAQTPQARARRESFIHELLDDVDVYPYTKDAALVAGKIDGEQQERGITIPF
                     SDLLIGAAALVHGYSVLTVNVRHFRLIPGLKVVSL"
BASE COUNT          528 a          794 c          761 g          511 t
ORIGIN      
        1 tacggaactg aacgggaact acgtttccag ggtagccttg agcggctata ggggcccctt
       61 cgactcggcc acccgccgcg gcggggcgcg gcctggctcc gtcgaaagtc attcaccacc
      121 cttcggcttc ggtcagggca ggctcagata cggagcgcac ggagttttgc cttctacgga
      181 gttttctcgg tgatctccgt gcctccgtgg ttaattttaa tttctatggg tgggccgcag
      241 acccatgtga tactcaggac gacaaacatg aaaaacaagc gttccgaacc ctcccgaaac
      301 ccaggtcaaa caggatggcg tggcaagcga acgaggacat gattcagttt cgcaaacgcc
      361 gatctcctct ggcaaagggt gacggcctcc cataatgggt cgtatgggca gtccctcctc
      421 atcgccggtg cgaattcgct gaacatttcc gaacgcatcg aatatattga ctgcaatgcc
      481 ttcactcgca ctttccgacc tgattggcgc gccggtaatc gacaacaccg gaacgaccgc
      541 cggcaaagtg cgcgaagtca cgctggtgcc tcaggagaat tccagccaaa tcgcgggctt
      601 tgtggtgaaa acccgccacg gcgaccgtct gctgcccacc aagtcgctca gcctgatcaa
      661 cggcggcctg cgggttgcta cccccgcttc agagtggggc ccttacgccg gtggtgaagg
      721 catgctcctg ctggagcgcg acctgctcga ccagcagatc attgacgtcc acgggcgcaa
      781 ggttgtgcgg gtcaatgaca ttgacatcca cgaggagctt ggccacgacc acattgtgct
      841 caagcttggc gcggtggacg tcggcgcgcg gggcgcggtc cgccgcctgc tgaagggcgt
      901 gattccggca gccgtgctgc aattgcttct caccaagatt ccgccgcgga tgatcccgtg
      961 ggaatttgtt gacctcatcg agattgatcc cgcccgccgc gtcaagctga agatttccct
     1021 tgatcgtctg gccaagctgc acccagccga cattgccgac atcgtcgaag acctcgcacc
     1081 cgccgagcgc gaagccatct tcgagactct ggacgatggg gtagccgccg aaaccctgga
     1141 agagatcgat cccaagatgc aggtttcgat tctcgcctcg ctcgactccg accgcgccgc
     1201 cgacattgtg gaggagatgg atcccgatgc cgccgccgac ttgctgggcg acatgcatcc
     1261 cgatgactcg caagagatcc tgaccgagat gcagccggag gagcgggccg aagtcgccgg
     1321 gttgatgaac ttcgaggaag atacggccgc cgggcgcatg accaccgact atatggcctt
     1381 gggaccggat gggaaggtgg aagacgcgat cgagatgctg cgcaacttcg agggcggcgt
     1441 ggaaagcatc agcacgatct atattgtcgg cgacaagaac aaactgctgg gcgccgttcc
     1501 gctggccaag attgtgcttg cgcccccggg cacgctgctg atgatgttga gcaccgggca
     1561 cctcaccacc gtgcatcccg gcgccgacga gaagacggtt gccgagctct tcgataagta
     1621 caacctgctc accctgcccg ttctggatga ggacggtaag ctgaccggcg tcatcaccgc
     1681 tgacgatgtc atcaacatgc tgcgggcgaa gctgtaacgg gaacgaagag gggtaagggt
     1741 ataatctcgg catgacccag acggtgatac gcatctcgga agcggaagcc gagagcgact
     1801 tcggctctgt gctggcgcgg gtgcgcgctg gcgcggaagt tatcatcgaa agcgagagcg
     1861 gcgggccgcc cattgcggtc gtacggccgg ttgggtcgcg tccgggccgc ctgctttccg
     1921 aatcgattgc cttggccgag gcccatgctt ctacggctac tctcgacgca gacttctccc
     1981 acgatctcga agcaattatc aatagccacc gtgagccgct caatccgccc gcatgggact
     2041 aatcctcgat tccagtgttg ccatcgccgc tgagcgccga ggcgataatg tgacccagct
     2101 tctcgatcaa attgttgcag ccgctgggaa ccaagaagca gtgctctctt ccattggttt
     2161 gacggaactc gtgcatggca tctatcgtgc gcaaacgcct caggctcgtg ctcgccgcga
     2221 atccttcatc catgaactat tggatgacgt cgacgtgtac ccctacacta aagacgcagc
     2281 actggtggct ggcaagatcg acggcgagca gcaggagcgc ggcataacca tccccttcag
     2341 cgacctgctg attggggcag cggctctcgt acacggttac tccgttctta ctgtcaatgt
     2401 gcgccacttc cggctaatcc ccggcctgaa ggttgtctct ttgtgacgac ggcctcgcga
     2461 ggcgacatcc cgcgactcgg cttcgcacac tcttcacgac tcaagtgcgc cgcttcctcg
     2521 aaatctacag gccgaagatc ggccttcccc acctcctgat agtcttttct aatcgaagaa
     2581 tgagcctgaa ggaa
//
LOCUS       DATMGX010000845         2159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_204505, whole genome shotgun sequence.
ACCESSION   DATMGX010000845 DATMGX010000000
VERSION     DATMGX010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204505"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="VNR65_13825"
     CDS             <1..1733
                     /locus_tag="VNR65_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWJ19797.1"
                     /translation="ALDDANRIAPNIYFNVKYPETLGRYKGDLIPVPLKDYVTGKLRR
                     SLIALWCAVGAILLIAGVNLSNLLLARAVARAKEFAVRGALGASRGRIVRQLLMESLV
                     LSSAGAALGLGLAVILIAWLAHQGSVALPLLGALRIDGQALGWTVLVAVFTAMIFGLL
                     PALRIASGNLQEMLKDSGPGAGLGRKHERVRAALVIFEVALACVLLVSAGLLLRSFVK
                     VLDVDLGFQPDHAASIKVDYDDSAPSEEARQAKRGEVFQQIIARVSALPGVEAAGMSD
                     YLPLGPNREWDTPVPKGNIFSPGELPDPLVYVITPGFIRALGIRMHGRDFTWADGPHS
                     ERVVLINASAARVYWPGEDAVGKILMRDKEEDRVVGVVDDIHEETVEGGTGSQIYYPA
                     MQQGPSGAQLVVRTSLPPATLAASVLRALRELNPRQPSAEFRPIQTIVDRAVSPRRFF
                     MLLVAAFAGLGLVLAALGIYGVISYSVTQQAQAIGIRMALGASVGHVQRQVLGGTLRL
                     AIAGMALGTLVALAVAKLISSLLFATSSWDLPTYLGMALALLLVAAISGYIPARRASG
                     VNPVEVLRSN"
BASE COUNT          372 a          699 c          631 g          457 t
ORIGIN      
        1 aggctctcga cgacgccaac cgcatcgcgc ccaatatcta tttcaacgtg aaatatccgg
       61 agacgctggg ccgctataaa ggtgatctca ttcctgtgcc gctcaaggat tacgttacgg
      121 gaaaactccg tcgatccctg attgcgttat ggtgcgcggt aggtgcgatt ctgctgatcg
      181 ccggcgtgaa cctctcgaac ctccttctgg ctcgtgccgt cgcgcgcgcc aaggagtttg
      241 ccgtccgtgg agcacttggg gcaagccgcg gtcgcatcgt gcgccagcta ctgatggaaa
      301 gcctcgtgct ctccagtgca ggagcggctc tcgggctagg attggctgtc attctgattg
      361 catggctcgc gcatcaggga tccgtggctc tgccgctcct cggcgccctt cgtatcgatg
      421 gccaggcact gggctggact gttctcgttg ccgtcttcac tgccatgatc ttcggcctgc
      481 ttccagcgct caggatcgcc agcggcaatc ttcaggaaat gctcaaggat tccggcccgg
      541 gcgcaggact tggccgcaag cacgaacgtg tccgcgccgc gctggtgatc ttcgaggtcg
      601 ctctcgcctg cgtgctgctg gtgagtgccg gcttgctgct gcgcagcttt gtcaaagtgc
      661 tcgacgtcga cctcggcttc cagcccgacc acgccgcctc catcaaagtc gattacgacg
      721 atagcgcccc ctccgaagaa gccaggcagg ccaaacgcgg cgaagtcttc cagcagatca
      781 ttgcccgcgt aagcgcgctg cccggcgtcg aggccgccgg catgtccgat tatcttccgc
      841 tgggaccgaa ccgcgaatgg gatacgcccg tgccgaaagg gaacatcttt tctcccggcg
      901 aactgccgga cccgcttgtc tatgtgatca ccccgggatt cattcgtgct ctcggcattc
      961 ggatgcacgg ccgcgacttc acctgggctg atggcccgca cagcgagaga gtcgtcctca
     1021 tcaacgcctc ggccgctcgc gtctactggc ccggtgagga cgccgtcggc aagattctca
     1081 tgcgcgataa ggaggaggac cgagtggtcg gcgtcgtcga cgatattcac gaagagacag
     1141 tggagggtgg aaccggttcg cagatttact atccggcaat gcagcaaggg ccgtcgggcg
     1201 cgcaactggt ggtccgcacc agcctgccgc ccgcaaccct cgctgcgagc gtcctgcgcg
     1261 ctttgcgcga gctgaatccc agacaaccat ccgccgagtt ccggcctatc cagactatcg
     1321 tcgaccgagc cgtctcacca cgccgcttct tcatgctgct ggtagcggca ttcgccggac
     1381 tcgggctggt gctggccgca ctcggcatct acggggtcat ctcgtattcg gtcacgcagc
     1441 aagcgcaagc catcggcatc cgcatggcgc tcggcgcaag cgtcgggcac gtgcagcgcc
     1501 aagtgctcgg cggtacgctg cgcctggcga ttgccggtat ggcgctgggc acattggttg
     1561 cactcgcagt cgccaagctg atttcttcgc tattgtttgc cacctcgtcc tgggatctgc
     1621 cgacgtatct cggaatggcg ctggctctgc tgctggtggc agccatctcc ggctacattc
     1681 ccgcccgccg cgcgtctggc gttaaccccg tggaggtgtt gcgcagcaat tgactgtaga
     1741 acgtggtgtc gccttcacgg caaacctttg atcgaacctc ggagtcgaaa cgctcggaga
     1801 agaccgcgcg tagcccttga tccaataaac ggtttttgag agcggagtct cgcctcgaag
     1861 actacaataa tgaaggccgc tgatgggtag gctgttatgc tctcttgcct ctttactccc
     1921 ggcaatccct ttcccggtgc tcgcatcgcc ttcgtcaccc tcctaaccct gttgctgtcc
     1981 ggatggacca cctgcactgc catcgttaac ttcggcagct gtcccggcac gatgcctcag
     2041 ccccaaatcg catcactcat gccggacacc gttccgaccg acgcggaatc agttccactg
     2101 atcgtgaatg gaagcggctt tgttccccaa tcgcagatca tgtggaacga aagcccgct
//
LOCUS       DATMGX010000846         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_149362, whole genome shotgun sequence.
ACCESSION   DATMGX010000846 DATMGX010000000
VERSION     DATMGX010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149362"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VNR65_13830"
     CDS             <1..222
                     /locus_tag="VNR65_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19798.1"
                     /translation="RWAEAASDQRAGVRVEGAPYIRLEGRNLRVLPSVVGRSEKNRRP
                     EAPEYRLDTSATAVFQGGFSVSLIGGEKK"
     gene            219..1805
                     /locus_tag="VNR65_13835"
     CDS             219..1805
                     /locus_tag="VNR65_13835"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HWJ19799.1"
                     /translation="MNVRHWPAAVFLAALCAMQAADQSPKWGDWPKWGDQGDGSYRNP
                     VIPADYSDIDCIRVGGDYYAISSTMQFSPGMVVLHSTDLVNWSILGHVVSDLTQIGPE
                     LNWSRMNRYGRGVWAGSIRYHARKFWVYFGTPDEGYFMTTAINPAGPWQPLHRVLEGA
                     GWDDPCPFWDDDGQGYLAGTSFRDSYKTHLWKLTPGGRDLVQESDRIIHQSKGSEANK
                     LYKFNGLYYHLFSEVHAEGRVVMMERSKNIFGPYTEIRQLNYADRPAMEPNQGGLVQT
                     ESGAWYWFTHHGTGDWEGRCASLVPVKWIDGWPIIGEPDEKSIGHFVWSGKTPVGGAQ
                     IPALQTSDEFGEARLSPQWEWNHQPRAEKWSLTERPGWLRLHGFRPLQPGDLRKAGNT
                     LTQRSFRTTANEVVVKFDLRGMADGQRAGLCHFSKDDSALGVFQSGAIRTIQYEHSGK
                     ITSGPAVKGTDLWVRSRWGLDGRSQYSYSLNGRSFTEFGEPYQLSWGNYRGDRIGLYS
                     YNEREEAGYVDVDYLHYTYR"
     gene            1867..>2178
                     /locus_tag="VNR65_13840"
     CDS             1867..>2178
                     /locus_tag="VNR65_13840"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HWJ19800.1"
                     /translation="MVDWYRTAAKKAAEHHIMIDFHGAFKPDGMRRTFPNVMTREGVM
                     GAEYNKWSGRETPKHDVMLAFTRMLAGPMDYTPGGFDNVTWENFTPRNRAPMVMHTRA
                     HA"
BASE COUNT          454 a          630 c          695 g          399 t
ORIGIN      
        1 cggtgggcag aggctgcttc cgaccagcgc gccggcgtcc gcgtggaagg cgcgccgtac
       61 atccggttgg agggacggaa tctacgcgtg ctgccctcgg tggttggacg ttccgagaag
      121 aaccggcggc cggaagctcc cgaataccgt ctggatacaa gcgctacggc cgtattccag
      181 ggcggctttt ccgtgagcct gattgggggc gagaagaagt gaacgttcgg cactggcccg
      241 ccgccgtgtt ccttgccgcg ctgtgcgcga tgcaggcggc ggaccagtcg cctaagtggg
      301 gcgactggcc gaagtggggc gaccagggag atggtagcta tcggaatccc gtaattcccg
      361 ccgattacag cgacatcgac tgcattcgcg tcggcgggga ttactacgcc atctcctcga
      421 ccatgcagtt ttctcccggc atggtcgtcc tgcactccac ggacctggtc aactggtcca
      481 tcctcggcca tgtcgtcagc gatctgacac agatcggtcc agaactgaat tggagccgga
      541 tgaaccgcta tggcaggggc gtttgggcag gatcgattcg ctatcacgcc cggaagttct
      601 gggtttactt cggcacgccc gacgaagggt atttcatgac tacggccata aacccggcag
      661 gaccgtggca gcctttgcat cgcgtgttgg aaggcgccgg ctgggacgat ccctgcccgt
      721 tctgggacga cgacgggcag gggtatctgg ctggaacttc tttccgcgat agctacaaaa
      781 cccatctctg gaaactcacg cccggtggtc gcgacctggt tcaggaatcg gaccgcatca
      841 tccatcagtc caaggggagc gaggccaaca agctgtacaa gttcaacggc ctctattacc
      901 acctgttcag tgaagtgcac gcggaggggc gcgtggtcat gatggaacgc tccaagaaca
      961 tcttcggtcc gtataccgag atccgccaac tgaactacgc cgacagaccg gcgatggaac
     1021 ccaaccaggg tgggctggtg cagaccgaaa gtggcgcgtg gtattggttc acccaccacg
     1081 gcacaggcga ttgggaagga cgttgcgcga gtttggtccc agtgaaatgg atcgatggct
     1141 ggccgatcat cggcgaaccg gatgagaaga gcattggcca ttttgtttgg tcgggcaaaa
     1201 cgcctgtggg gggcgcccag attcccgcgc tccagaccag cgacgaattc ggcgaagcgc
     1261 gcctgtctcc gcagtgggaa tggaatcacc aaccgcgtgc ggaaaagtgg tcgctgacgg
     1321 agcgtccagg atggttgcgc ctgcatgggt tccggccgct ccaacccggc gatttaagga
     1381 aggccggcaa caccctgacc caacgctcgt ttcggaccac agccaatgaa gtggtggtca
     1441 agttcgatct tcgcggcatg gcggacgggc agcgagccgg cctgtgccac ttctccaaag
     1501 acgactccgc tcttggcgtc ttccagagcg gggcgattcg aacgatccag tacgaacata
     1561 gcggcaagat cacatccgga cctgcggtaa agggcactga cctatgggtg aggtcacggt
     1621 ggggattgga cggcaggagc caatattcct acagcctgaa tggccgctcc ttcacggagt
     1681 ttggagagcc ttaccagctt tcctggggca actatcgggg agatagaatc ggcctctaca
     1741 gctacaacga gcgggaagag gccggctatg tcgacgtgga ttacttacac tacacttaca
     1801 ggtagattgc cggccacctg acatcagttg cctgcgccga ttagttccat aagcagcgac
     1861 cagtggatgg tggactggta ccgcaccgcg gcgaagaaag cggcggagca tcacatcatg
     1921 atcgacttcc acggcgcgtt caagccggac ggaatgcggc gcacctttcc caacgtgatg
     1981 acgcgcgaag gcgtgatggg cgccgaatat aacaaatgga gtggacgcga aacgccgaag
     2041 cacgacgtca tgctggcgtt cacgcgcatg ctggccggac cgatggacta tacgccgggc
     2101 ggcttcgaca acgtcacctg ggagaacttc acgccgcgca accgggctcc gatggtgatg
     2161 cacacgcgcg ctcacgcc
//
LOCUS       DATMGX010000847         2806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_263339, whole genome shotgun sequence.
ACCESSION   DATMGX010000847 DATMGX010000000
VERSION     DATMGX010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263339"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2460
                     /locus_tag="VNR65_13845"
     CDS             <1..2460
                     /locus_tag="VNR65_13845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWJ19801.1"
                     /translation="AAPSEPQQHPEEGDQEPAGRGEPMALEAILPPTIYEEFTAAVAR
                     AEEARDEIPAHPDDVEEAPAHKEEDKGAGPATEANGELAAKPKPDQASSTDEDHTNWE
                     NGSASDDGGIISVPETPSPAPVEVVGSEDALEELPQRHKRRWRHYKIQEVIKRNQIVL
                     IQVVKEERGSKGAALTTYLSLAGRYTVLMPNTASGGGISRKITVPADRKRLKEIALEL
                     EVPEGMGLIIRTAGASRTKAEIKRDFEYLLRLWENVRDLTLKSTAPCLVYEEGDLIKR
                     SIRDLYNKDIDEILVAGEESHRDAKEFMRMLMPSHAKNVLLYKEPEPLFIKYEVERQL
                     NQMFSPVVTLRSGGYIVLNQTEALVAIDVNSGRATREYSIEDTALKTNLEASEEVARQ
                     VRLRDLAGLIVIDYIDMDERRNNRAVERKLKECLRHDRARIQVGHISHFGLLEMSRQR
                     LRQGVVEVSTMPCPVCQGVGHIRSSESVALMILRSIEDHLRTQGPANLTVSASNEASL
                     YILNHKRAYLRDIEMRYGVTIVIQLDEKAHGAHFSLERGAEAVPPAQGETKAVHMEAP
                     SLPSAEEEEEAVEEGDDKRPARRKRRRRRGRGEQSFEPMLNGSGALSSEAESDEEDEA
                     EDEVQIPVQAQAEAQHGLQSAPLQNGVEGETEGQLRRNRRRGRRGGRRHRDEVGSPEY
                     ARSHTAPIPGLGEQPTIDYNHDFPKNLMLGPISPRASLPEPEQLMEPEIEQAPTQVAA
                     IPQTEAPADQQAAGEPEIPTVAEADGSADQPVEAAAASEQDEEPAVELTVVPHARAPE
                     PKPKPVRTAPPRKGWWQRRIG"
     gene            2477..2659
                     /locus_tag="VNR65_13850"
                     /pseudo
     CDS             2477..2659
                     /locus_tag="VNR65_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015887115.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          652 a          855 c          838 g          461 t
ORIGIN      
        1 gcggccccgt cagagcctca acagcatccc gaggaaggtg atcaggagcc tgccggtcgg
       61 ggcgagccga tggcgttgga agctatcctt cctccaacga tctatgagga attcaccgcc
      121 gcagtagctc gtgcagagga agcgcgcgat gaaatccccg ctcaccccga cgacgtcgag
      181 gaagctcccg ctcataaaga agaggataag ggggcgggac ctgcgacgga ggcgaacggc
      241 gagctagccg ccaagccaaa gccagaccaa gcctcttcca cagacgaaga ccacaccaat
      301 tgggaaaacg gatccgcttc agacgatggg gggatcatct ccgttcctga gactccaagt
      361 cctgctccgg tcgaggttgt cggctctgaa gacgcgttgg aagaactacc ccagcgtcac
      421 aagcggcgct ggcgccacta caaaattcag gaagttatca aacgcaacca gatcgtcctg
      481 atccaggtcg tcaaggagga gcgcggcagc aagggcgcgg cgctcaccac ctacctgtcg
      541 ctcgcgggac gatatacggt cctgatgccg aatacggcga gtggcggcgg tatttcgcgc
      601 aagatcactg tcccggccga ccgcaagcgc ctgaaggaaa ttgcgctcga gcttgaagtt
      661 ccggaaggca tgggcctcat tatccgcaca gccggcgcct cccggaccaa ggcggaaatc
      721 aagcgcgact tcgagtatct gttgcggctt tgggaaaacg ttcgtgatct taccctgaaa
      781 tcgactgccc cctgcctcgt ttacgaggaa ggcgacctta tcaagcgatc catccgcgac
      841 ctttataaca aggacatcga cgaaatcctt gtcgccggcg aagaatcaca ccgcgacgcc
      901 aaggagttta tgcgcatgct catgccgagc catgcgaaaa acgtgttgct ctacaaagag
      961 ccggaaccgc ttttcataaa atacgaggtt gagcggcaac tgaaccagat gttcagccct
     1021 gttgtgacat tgcggtccgg cggctacatc gttctcaacc aaaccgaggc cctcgtcgcg
     1081 atcgacgtga actccggccg agccacccgc gaatattcaa tcgaagatac cgcccttaaa
     1141 accaacctcg aagcctctga ggaagtggcc cggcaagtgc gcctgcggga cttggcggga
     1201 ctgatcgtta tcgactacat cgacatggat gagcggcgca acaaccgcgc cgtcgagcgc
     1261 aagctcaagg aatgtctgcg ccacgaccgg gcgcgcattc aggtcggcca tatcagtcat
     1321 tttggccttc tggaaatgtc gcggcagcgg ctgcgccaag gcgtcgtcga agtgtccacc
     1381 atgccctgcc cggtttgcca aggagtgggc catatccgct cgagcgagtc tgtggcgttg
     1441 atgatccttc gctcgataga ggaccatctc cgcacgcagg gaccggccaa ccttacggta
     1501 agcgcctcaa acgaagcctc cctttacatc ctcaaccaca agcgcgccta ccttcgcgat
     1561 atcgaaatgc gctatggggt aacaattgtc atccaactgg atgagaaggc gcatggggct
     1621 cacttctccc tcgagcgcgg agcggaagcc gttccgccag cccagggtga gacgaaggcc
     1681 gtgcatatgg aagcaccgtc gcttcctagc gccgaagaag aggaagaggc tgtcgaagaa
     1741 ggtgacgaca aacgcccggc acgccgcaag cgcaggcgca ggcgtggccg tggagagcaa
     1801 tctttcgaac ctatgctgaa tggaagcggt gctctttcca gcgaagccga aagcgacgag
     1861 gaggatgaag ccgaagacga ggtacaaatt ccagtccaag ctcaggccga ggcgcagcac
     1921 gggcttcaat ctgcccctct tcaaaacggc gtggaaggcg agactgaagg ccaactccgg
     1981 cgcaatcggc gcagaggcag aagaggaggg cgccggcatc gcgacgaggt gggctcgccg
     2041 gagtacgctc gctcccatac cgctcccata ccgggtctcg gggaacaacc cacaatcgat
     2101 tacaatcatg attttccgaa gaatctgatg cttggcccga taagcccgcg cgcaagcctg
     2161 cccgagcctg aacagttaat ggagccggaa atcgaacaag ctccaacgca agtggcggcc
     2221 ataccgcaaa ccgaagctcc tgccgaccag caagctgcgg gggagccgga aatcccaact
     2281 gttgcagagg cggatgggtc tgcggatcag ccggtagaag cggcagccgc atcagagcag
     2341 gacgaagagc ctgccgtgga actcactgtc gtcccacacg ctcgggcgcc cgagccaaag
     2401 ccgaagccgg tcaggaccgc gccgccccgc aagggctggt ggcagcgccg gatcggctaa
     2461 ggctactcgc aggcggggca gcccgccctt gccgacgcgg ttgatggcgg ggctgcacat
     2521 cctgaagtac agcgagaacc tgtcggaaga gcgtctgtgc gaggtttggg ccgagaactc
     2581 ctactttcag tatttctgcg gcgaggagtt cttccagcac aagctcgtct tcgatcgctc
     2641 gttgctgacc cgctggcggc agcatggacc gagttcggcg cgaaagtcag cctcgccacc
     2701 accgttgcgc cgtcaaaaag gcggctagtt catccgacgt cggaaaaagc gtcaccccgg
     2761 cgataaaacg caagctcaaa cggcgcgcct ccgtcgagcc tgtcat
//
LOCUS       DATMGX010000848         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_460189, whole genome shotgun sequence.
ACCESSION   DATMGX010000848 DATMGX010000000
VERSION     DATMGX010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460189"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..729
                     /locus_tag="VNR65_13855"
     CDS             190..729
                     /locus_tag="VNR65_13855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HWJ19802.1"
                     /translation="MNRRAFLQAALVAAAGAGVACTTNGTPWRFLTLDEARTLAAISD
                     QIIPSDQDPGAAWAGVVNYIDRQLCGPFENLQQTYRRGIAGIDESSRLISGRAFADLN
                     AQQQIDVLHRLEGGQAPGAVWKQASSSEFFALVIDHTMQGYYGDPRHGGNRDGVSWKM
                     LGLPYPPIRGRQKYDLTKP"
     gene            902..>2064
                     /locus_tag="VNR65_13860"
     CDS             902..>2064
                     /locus_tag="VNR65_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWJ19803.1"
                     /translation="MPLKHVNAIVIGGGAGGGIVAKELAEAGLSVVLLERGKWYTSYD
                     DRKDDLRNQRTCVLGNSFGPDEQRNPRVSVDAKGTGHIVRSNQGAYSNNAACVGGGTL
                     SYGAMAWRFLEKDFRLRSTYGAVEGSTLEDWPISYSDLEPYYEKAEWEIGVSGDDSNN
                     IFRAPRRRPLPMPPLPPNREHQILKPAAERLGLHPFDLPMLRNSVPYNGRGACMRCRW
                     CVGFACEVDAKCGTQNTVIPKALKTGNCELRSECIAKEVLTNDHGRATGVAYFDSKGR
                     LQEQSADLVVVSCSATESARLLLNSWSRLFANGLGNRYDWVGRNLQGHAYTGAAGLFE
                     EETYDDLGPGASIALCDYNHGNPGFIGGGLLANEFIRLPYQFSGLRAPGVPRWG"
BASE COUNT          417 a          649 c          609 g          389 t
ORIGIN      
        1 cagtcgccgg cgttcctgct caggatcaat cgtcgccatg gctggaaatc atactccact
       61 tgacggcccg ggaattccgc gtctgcccac gattccagca aatcgagcag cccacactcc
      121 ctcgtgatgc tgatcacaga cttcgccgct cctgcatgta acaaacggaa agtggaagcc
      181 agcctcaaat tgaaccggcg tgccttcctg caggctgcgc ttgtcgcggc ggccggagcg
      241 ggtgtggctt gcaccaccaa cggaactccg tggcgattcc ttacgctgga tgaagcccgc
      301 actctcgccg cgatcagcga ccagatcatt ccgtccgatc aggatccggg cgcggcttgg
      361 gctggcgtcg tcaattacat cgaccgccag ctgtgcggcc cgtttgaaaa cctgcagcaa
      421 acgtatcgcc gtggaattgc cggcatcgac gagagcagcc gcttgatttc tggcagggcc
      481 ttcgcagatc tgaatgctca acaacaaatc gatgtgctgc atcgcctgga gggcggccaa
      541 gctcccggcg cagtctggaa gcaggcttcc tcatcggagt ttttcgcgct tgtaatcgac
      601 cacaccatgc agggctacta cggcgatccc cggcacggag gcaatcgcga cggggtgagt
      661 tggaagatgc tgggccttcc atacccgccg attcgcggac gtcagaaata tgacctcaca
      721 aagccgtagt gcgacattga gtcgaggcca acaagaatgc aggctcttga atgccgaaag
      781 atcagtcctg aaatgagtgt cgaaggaatc gattttgggt ggcgcagcgc tttaagcgct
      841 gcgatcacgc gctcttttca gcggcggctt tagccgctgg ggacgggtag tcaggaatcc
      901 gatgcctctc aagcatgtaa acgcgattgt gatcggtggc ggcgccggag gcggcatcgt
      961 cgccaaagag cttgccgaag ccggcctcag cgtcgttctg ttggaacgcg gcaaatggta
     1021 cacgtcctat gacgatcgca aagacgacct gcgcaaccag cgcacctgcg tcttgggaaa
     1081 ttcctttggt cccgacgagc aacgcaaccc gcgcgtttcc gtcgacgcca aaggcacggg
     1141 acacatcgtc cgctcaaacc aaggcgctta cagcaacaat gccgcctgcg tcggcggtgg
     1201 cacgctcagc tacggggcga tggcgtggcg ctttctggaa aaagatttcc gcctgcgttc
     1261 cacctatgga gcggttgaag gcagcacgtt ggaagattgg cccatcagct actcggacct
     1321 tgagccctac tacgaaaaag cagagtggga aatcggcgtc tcgggcgacg actcgaacaa
     1381 catctttcgc gccccgcgcc gccgcccgct gcccatgcct ccgctgccgc ccaaccgcga
     1441 acaccagatt ctgaagcctg ccgccgagcg cctcggcctg catccgttcg accttcccat
     1501 gctgcgaaac tccgttccct acaatggacg cggcgcctgc atgcgctgtc gctggtgcgt
     1561 gggcttcgcc tgcgaggtcg acgccaagtg cggcacccag aacacggtca tcccgaaggc
     1621 actgaagacg ggcaactgcg aactgcgctc ggaatgcatc gccaaggaag ttctgacgaa
     1681 cgaccacggc cgcgcgaccg gtgtcgccta cttcgactcc aaggggcgcc tgcaagaaca
     1741 gagcgcagat ctggtggtgg tttcctgttc ggccacagag tcagcccgat tgttgttgaa
     1801 ttcctggagt cgtctcttcg ctaacggcct cggcaaccgc tacgactggg tgggaagaaa
     1861 cttacaaggt cacgcctata ccggcgccgc gggcctgttc gaggaagaga cctacgacga
     1921 cctgggcccc ggcgcgtcga tcgcgctctg cgactacaac cacggcaatc cgggattcat
     1981 cggcggcgga ctgctcgcca acgagttcat tcgcttgccg tatcaattct ccggattgcg
     2041 tgctcccggc gttccgcgct gggg
//
LOCUS       DATMGX010000849         3150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_454553, whole genome shotgun sequence.
ACCESSION   DATMGX010000849 DATMGX010000000
VERSION     DATMGX010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454553"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="VNR65_13865"
     CDS             <1..1339
                     /locus_tag="VNR65_13865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ19804.1"
                     /translation="IKSEWLHNIFTRIRTVKDVIETTPGEIHSKILQSSSSWALKFSI
                     DTEPLSRTEEETHSEAVQQELRKVFGESAEEVDVTVLPSSNDESFLEAILESLWRDIK
                     RTFSLERTHRSAPPARPTYANVSAPLKSGQWLNVVVTPRGGGPHASPLLVQFATMAAI
                     SAIGIVLVLSRLTKPLKELAQAAAALGRGESAAKLEERGPREVQDTIRAFNDMQERLS
                     RFVLDRTKMLAALGHDLRTPITTLRLRAEFIEEDEVRQKILQTLDEMLEMSEATLSFA
                     REEAAQEKTRLVDVGALISTVCADLADTGYPIECADTGSFTIRCRPSGLKRAFRNIVE
                     NAVAYGRRARVFVEHKRGDVAVMIDDDGPGIPTSDMERVFGPFVRLENSRNMDTGGVG
                     LGLAIARSIVRNHGGDIALQNRAEGGLRVTITLPGVTVIETPGNLPSESVAAV"
     gene            1432..1758
                     /locus_tag="VNR65_13870"
     CDS             1432..1758
                     /locus_tag="VNR65_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19805.1"
                     /translation="MYQFAYSEICEELPSQNVPKTRRLSDAIELIEAVQASPSRSHEW
                     LGVLSDFRKLWLTIVEDLPAGNHDGADVQPQDVWLADGVLQEIERCRFHKPEILAYAW
                     GGMWPQ"
     gene            1755..2216
                     /locus_tag="VNR65_13875"
     CDS             1755..2216
                     /locus_tag="VNR65_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19806.1"
                     /translation="MTSRGAFAPENTMSSVSLAEIERTLGAALKDLASELRLIDATDF
                     AAFIRIGHMANLRSLVQSSAELHFKPGTLELAELGEIDLGWLKPPLITLPMKFRHSGV
                     RVYFRLRLAALVAAVEVESITAENGVEGEDLNLKLRDALRDARTKRPAPPA"
     gene            2503..>3150
                     /locus_tag="VNR65_13880"
     CDS             2503..>3150
                     /locus_tag="VNR65_13880"
                     /inference="COORDINATES: protein motif:HMM:NF026047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19807.1"
                     /translation="MGPFPHDGPPATISDDNPMGTDGFEFVEFAHSEPAKLGALFELM
                     GFSAVARHRSKDVTLYRQGGVNYVVNAEPDSFAVEFAKGHGPCACAMGFRVVDAAHAL
                     KRAVALGAKPVSAPVGPMELNIPAIEGIGGALIYFVDRYGEKGSIWDVDFRWTGPRDP
                     QPDGAGLYYIDHLTHNVQRGRMDWWANWYRRFFNFRQIRYFDIEGKLTGLHSRAMT"
BASE COUNT          646 a          892 c          957 g          655 t
ORIGIN      
        1 gataaaaagc gagtggttgc acaacatctt cacacggatt cgcacggtca aagatgtgat
       61 cgagacgaca ccgggcgaaa ttcacagcaa gatactgcaa tcctccagtt catgggcttt
      121 gaagttctca attgacacgg agcctttgtc gcgcaccgag gaagaaacgc attcggaagc
      181 tgttcagcag gaactcagga aggttttcgg agagagcgcc gaggaggtcg atgtcaccgt
      241 tctcccatcc tcaaacgatg aatccttctt ggaggcgatt cttgaaagtc tctggcgaga
      301 catcaagcgc acattttctc tggaacgcac tcaccgttcc gcacctcctg cgcggccgac
      361 ctatgcgaac gtatccgctc cattgaagag tgggcaatgg ctgaatgtcg ttgtaacgcc
      421 ccgagggggc gggcctcacg cttcgccatt gctcgtccag ttcgcaacca tggcggcgat
      481 ctcggcgatt ggcatcgtgc tggttctaag ccgcctcacc aagccgctca aggaattggc
      541 gcaagcggca gcggcattgg gccggggaga gtcggctgcc aagctcgaag aaagagggcc
      601 gcgcgaggtg caggacacaa ttcgcgcctt caatgacatg caggagagat tgagccggtt
      661 cgtgcttgat cgaacgaaga tgctcgccgc ccttggacac gacctgcgca cgccgataac
      721 gactttgcgc cttcgcgctg aattcatcga ggaagatgag gtcaggcaga agatattgca
      781 gacgctcgat gaaatgctcg agatgtccga ggcgacgctc tctttcgctc gcgaggaggc
      841 agcgcaggaa aaaacccggc ttgtcgatgt cggggccttg atttccaccg tttgcgccga
      901 cctcgccgat accggctacc cgatcgaatg cgccgacacc ggcagcttta cgatccgctg
      961 ccggccttcg ggattgaagc gggcctttcg gaacatcgtt gagaatgcag tggcctacgg
     1021 ccgcagggcg cgcgtcttcg tcgaacataa acgcggggat gttgcagtca tgatagacga
     1081 cgacggtccg ggcattccaa caagcgatat ggagcgagtc ttcggcccgt tcgttcgtct
     1141 ggagaattcg cggaatatgg acacgggcgg cgttggtctg ggcctcgcaa ttgcccgctc
     1201 catcgttcgc aaccatggcg gcgatattgc gctgcaaaat cgcgccgaag gtgggctgag
     1261 agtcacgatc accctccccg gcgtcacggt cattgagacg cccggtaacc tcccctcgga
     1321 gagcgtggcc gccgtctgag acctcctcca atcgcatcca gcgactcacg tttggcataa
     1381 gcgtcacgtg cgataagcgc aacgcccaca gtactcaatg gagaggaagt catgtatcag
     1441 ttcgcttatt cggagatttg cgaagagttg ccctcacaaa atgtcccgaa aacgcgcagg
     1501 ctctccgacg cgatcgaact gatcgaggcg gtgcaagctt cgccctctcg atcccatgaa
     1561 tggctcggcg ttctttcgga cttccggaag ctgtggctta cgattgtgga ggaccttccc
     1621 gcgggcaacc atgacggggc cgatgtccag ccgcaagacg tgtggcttgc ggacggggtt
     1681 ctgcaggaga tcgagcgttg ccgcttccac aagcctgaaa tccttgcata tgcttggggg
     1741 gggatgtggc cccaatgaca tcgcggggcg catttgcgcc tgaaaacacc atgagttcgg
     1801 tttctcttgc cgagatagag cgaacgcttg gggcggccct gaaggatctg gcttcggaac
     1861 tcaggctcat cgatgccacg gacttcgcgg cgttcatccg gatcggccat atggcgaatt
     1921 tgcgaagcct tgtgcaatcc tcggccgagc ttcatttcaa acccggaacg ctggagcttg
     1981 ccgagcttgg ggaaatcgat ctcggatggc tcaagccgcc gctcatcacc ttgccgatga
     2041 agtttcgcca tagcggcgtt cgggtctact tccgcctgcg tttggccgcc ctcgtggcgg
     2101 ccgtggaagt ggagtcgatc acggccgaaa atggagttga gggcgaggat ttgaatctga
     2161 agctccggga tgcgcttcgg gatgctcgca ccaaacggcc ggcgccgccc gcgtaaagtc
     2221 cggcatcttc gcaggaaaga taattgttta catatgtagt tacccccagt ttacaaaacg
     2281 aaaccaatct taagcctatt ttaagtatcg caaaggtcac agaataacag gcagaggctt
     2341 gggctctcgc gcgggtttag ccgcctgagg ggcgcctgga tgactttcgc gtgaaggtcc
     2401 ggaaggggca ggcgtcgagc gcaggcgcgc catttcaaac ctgtccccgt gacccatatc
     2461 atcatcgggg gaggagaggt ccggcgaagg aggcgttctg cgatggggcc gttcccccac
     2521 gatgggccgc cggcaaccat atcggacgac aatccgatgg ggaccgacgg ttttgagttt
     2581 gtcgagtttg cccattcgga gccagccaag ctcggcgccc ttttcgaact gatgggcttt
     2641 tcggccgtcg caaggcatcg ttccaaagac gtcaccctct atcggcaggg cggcgtgaac
     2701 tacgtcgtaa acgccgagcc ggacagcttc gccgtcgaat ttgcaaaagg tcatggaccg
     2761 tgtgcctgtg caatggggtt ccgcgttgtc gatgcggctc acgcgctcaa acgggcggtt
     2821 gcgcttggcg ccaagccggt ttcggcccct gtcggtccga tggaactcaa catcccggct
     2881 atcgagggta taggcggcgc gctcatctac tttgtcgacc gctatggtga gaagggttcg
     2941 atctgggatg tcgattttcg ctggacgggt ccgcgcgatc cgcagccgga cggtgccggt
     3001 ctctattata tcgatcacct gactcataat gttcagcgcg ggcgtatgga ttggtgggcc
     3061 aactggtaca ggcgcttttt caacttccgc cagatccgtt acttcgacat cgagggcaag
     3121 ctcacaggcc tgcactcgcg ggccatgacg
//
LOCUS       DATMGX010000850         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_177653, whole genome shotgun sequence.
ACCESSION   DATMGX010000850 DATMGX010000000
VERSION     DATMGX010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177653"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..886)
                     /locus_tag="VNR65_13885"
     CDS             complement(68..886)
                     /locus_tag="VNR65_13885"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWJ19808.1"
                     /translation="MSALADKALVLLEWYRAMGVDEAIAEAPQDWFARQPQAVGSPAP
                     LGGVSAQEIKRKPLERASAPLPQPASQTTLETSADVMSARELARRAASLDELRSLLEN
                     FEGCGLKKTASRLCLSRGSPKARLMLIGEAPGKDEDVQGEPFVGRAGQLLDRMLAAVN
                     LTEADVYITNLVFWRPPGNRTPSPEEVQVCQPFVERQIELLSPKILVFLGNAAAKQLT
                     GATEGIMKLRGKWLNYSGIRAMATLHPAYLLRTPIAKRLAWRDLLAIRDALDQS"
     gene            995..>2208
                     /locus_tag="VNR65_13890"
     CDS             995..>2208
                     /locus_tag="VNR65_13890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ19809.1"
                     /translation="MEANGVDVIAEELELPERETMEFDVVIVGAGPAGLAAAIRLKEL
                     AAAKGTDLSVVVLEKGSEVGAHILSGAVFDPIGLNALFPDWKEKGAPLTQHVTKDKFY
                     YLGERGATSIPHFLMPPLMSNKGSYIVSLGNVVRWLGEQAEALGVEIYPGFPAVEVLK
                     DSEGRVIGVATGDLGVGRDGKPKDEFVRGMALLGKYTLIAEGARGSLAKKLVRDFGLD
                     TNCTPQKYGIGLKELWQVKPEKHKPGLVQHTLGWPLEDRTGGGSFMYHYGENLVSLGF
                     VVHLNYENPYTSPFHEFQRLKTHPLIATVLEGGKRIGYGARAIVEGGWQSIPKLTFPG
                     GALIGCSAGFVNVPRIKGSHNAILSGVAAAESAFEALQAGRSGDELLAYEERVRTGPI
                     ARDLKPVRNVKPL"
BASE COUNT          430 a          659 c          722 g          397 t
ORIGIN      
        1 aagcaggcgc agaagggagg tcgtggatcg cggctttgaa atcataagag atcaacacgc
       61 aaaaagttta agattggtcc aacgcgtcgc ggatggcgag gaggtcgcgc caggcgagcc
      121 gcttcgcgat gggcgtgcgt aggagataag cggggtgcag cgtcgccatg gcacggatcc
      181 ccgaataatt caaccacttc ccgcgcagct tcataatgcc ctcggtcgcg ccggtgagct
      241 gcttggcggc ggcattgccg aggaaaacga ggattttcgg gctgagcagc tcgatttggc
      301 gctcgacgaa aggctggcag acctgcacct cctccggcga tggcgtgcga ttcccagggg
      361 ggcgccagaa tacgaggttg gtgatgtaga catcggcctc ggtgaggttg accgcggcga
      421 gcatgcggtc gagaagctgc ccggcccggc cgacgaaagg ctcgccctgg acgtcctcat
      481 cctttcccgg cgcttctcca atcagcatga ggcgcgcctt cggggagcca cgcgagagac
      541 agaggcgcga ggccgttttc ttcagcccgc agccctcgaa attctctaaa agcgagcgca
      601 actcatccag gctcgcggct ctcctcgcga gttcgcgggc gctcatcacg tcggcggagg
      661 tttcgagcgt ggtttgagat gcaggctgcg ggagcggagc gctggcccgc tctaaaggtt
      721 tccgtttgat ctcctgcgcg gagactccac ctaaaggggc agggctgccg actgcctgag
      781 gctggcgggc aaaccaatcc tgtggcgcct cggcgatggc ctcgtcgacg cccatcgccc
      841 tgtaccattc gagcaggacc aaagccttat ccgcaagcgc gctcatcggg gcacaatgcc
      901 acggtgcgcg gattaatcaa gttggcgcag ttgtgcgttt aaatgtaaac aagacttgcc
      961 agccgcgtaa cgtccggagc agggggagga cgatatggaa gccaatggtg tggacgtgat
     1021 cgccgaggag cttgagcttc cggagcggga gacgatggaa ttcgacgtcg tcatcgtcgg
     1081 cgccggacct gccgggcttg cggctgcgat ccggctgaag gagcttgcgg cggcgaaagg
     1141 caccgatctt tccgtcgtcg ttctggaaaa aggctctgag gttggcgcgc acattctttc
     1201 gggcgcggtt ttcgatccca ttgggctcaa tgctcttttc cctgactgga aggaaaaggg
     1261 cgcgcccctg acccagcatg tgacgaagga caagttctac tatctcggag agcgcggcgc
     1321 gacgagcatt ccgcatttcc tgatgccgcc gctgatgtcg aacaagggca gttacatcgt
     1381 cagcctcggc aatgtggtgc ggtggctggg cgagcaggcc gaggcgcttg gcgtcgaaat
     1441 ctacccaggc tttcccgccg tcgaggtcct gaaggacagc gaaggacgcg tcatcggcgt
     1501 tgccacgggc gatctcggcg ttggccgcga cggcaagccg aaggacgaat tcgtgcgcgg
     1561 catggcgctt ctcggcaaat acacgctgat cgccgaaggc gcacgcggat cgctcgccaa
     1621 gaagctcgtc cgcgatttcg gcctggacac gaactgcacg ccccagaaat acggcatcgg
     1681 cctcaaagag ctgtggcagg tcaagccgga aaagcataag cccggattgg tacagcacac
     1741 attgggctgg ccgctcgaag accgaacggg tggcggctcc ttcatgtatc actacggcga
     1801 gaacctcgtc tcgctggggt tcgtcgttca cctcaactat gagaacccgt acacaagccc
     1861 cttccacgaa ttccagcgcc tcaagacgca ccccctcatt gcgacggtcc tggagggtgg
     1921 caagcgcatc ggctatggcg cgcgcgccat cgtcgagggc ggctggcagt ccattccgaa
     1981 gctgaccttc ccgggcggcg cgctcatcgg atgcagcgct ggcttcgtca atgttccccg
     2041 catcaagggg tcgcacaatg ccatcctgtc tggcgtcgcg gccgcagaga gcgcattcga
     2101 ggcgcttcag gcaggcagaa gcggggacga actcctggcc tacgaagagc gtgtgcgcac
     2161 gggtccgatc gcgcgcgacc tcaagcctgt tcgcaacgtc aaaccgct
//
LOCUS       DATMGX010000851         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_119460, whole genome shotgun sequence.
ACCESSION   DATMGX010000851 DATMGX010000000
VERSION     DATMGX010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119460"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VNR65_13895"
     CDS             complement(<1..699)
                     /locus_tag="VNR65_13895"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HWJ19810.1"
                     /translation="MSLKSLTRLLIGAFICGAILVSARAFAAEEAFVTNQNDNTVAVV
                     DLDRMAVFTRLAIPGNPVGVAVSRDGNRVYVTSSEGKTLSVIDAVERKVVNVIHLKGG
                     PFGVAVHPSGRPVYVTDWFEHRIWIISPETGEVTAEVHVGNSPSGIAVTADGSTIVTA
                     DRDSDQLSFIDAKTLKVKATAKVGSKPFGVTIDDKGGRAFTANVGSNSVSVVDIASGT
                     VLAEVKTGERPYAVA"
     gene            complement(696..1250)
                     /locus_tag="VNR65_13900"
     CDS             complement(696..1250)
                     /locus_tag="VNR65_13900"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWJ19811.1"
                     /translation="MQTTLTLRMTGSLVAAALLAFPTLAMAHGPSRQKLIESVEINAP
                     AEKVWAKIGNFQDMSWHPAIEKTEGQGGNEKGATRKLTLKGGGVIEESLEKHDNGKMS
                     YTYRITNVDVKVLPVTNYSSWLTVEPEEGGKSKVEWKGAFYRGFPNNEPPADLNDEAA
                     IKAVTGVYRAGLDTLKKSLEAGGS"
     gene            1872..2270
                     /locus_tag="VNR65_13905"
     CDS             1872..2270
                     /locus_tag="VNR65_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19812.1"
                     /translation="MSIENFSAVLGKLSENGGNPELDGPAFRVSTKVAEVMLSLGAAI
                     ARNAVRASPYYEETLDSAPVPAPLSLSELKEQVTRATSIAELRGLRRRFARCSHPDRH
                     NDSEDSTATSEMAAANQLIDKAISSMRCPR"
     gene            2429..2701
                     /locus_tag="VNR65_13910"
     CDS             2429..2701
                     /locus_tag="VNR65_13910"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWJ19813.1"
                     /translation="MFHRCVLDGDLNASLLQDVWSNVEKLSEASEDVILDLSRVNFMD
                     SHGVGAVVALMRRLGLKGLQLKVIGLHGQPLRLFLDLHLVPVSGGC"
BASE COUNT          578 a          757 c          779 g          634 t
ORIGIN      
        1 agccacggcg taaggacgct cgccggtttt cacttcggcc agaaccgtcc cgctcgcgat
       61 gtcgaccacc gagaccgagt tgcttccgac gttcgccgtg aaggcgcggc cgcccttgtc
      121 gtcgatcgtc acgccgaacg gcttggagcc tactttggcg gttgctttga ccttcagcgt
      181 cttcgcatcg atgaaagaga gctgatcgct gtcgcggtcg gcggtcacga tggtgcttcc
      241 gtctgcggtc acagcgatgc cggaaggcga atttccgaca tggacctcgg ccgtgacttc
      301 gccggtttcg ggcgagatga tccaaatgcg gtgctcgaac cagtccgtga cataaaccgg
      361 ccgccccgag ggatgcaccg ctacgccgaa ggggccgccc ttgagatgaa tgacgttcac
      421 gaccttgcgt tcaaccgcgt ctatgacgga aagcgtcttg ccttccgaac tcgtaacata
      481 gacccggttt ccgtcacgcg aaacggccac gccaacggga ttgccgggga tcgccaacct
      541 tgtgaacacg gccatgcggt ccagatcgac gacagcaacg gtattgtcat tctggtttgt
      601 gacgaaggct tcctcggccg cgaaggcgcg ggcgctgaca aggatcgcgc cgcagatgaa
      661 ggctccgatg aggaggcggg ttaagctctt caaactcatg aaccgccggc ttcaagcgat
      721 ttcttcaatg tatcgagccc ggcccggtag acgcccgtga cggccttgat cgcggcctcg
      781 tcgttgagat cggccggtgg ttcgttgtta ggaaagccgc gatagaaggc gcccttccac
      841 tccaccttgc tcttcccgcc ctcttccggt tcgacagtca gccaggagga gtagttcgtg
      901 accggcaaga ccttcacatc gacattcgtg atccggtagg tgtaggacat cttcccgttg
      961 tcatgctttt cgagggactc ctcgatcacg ccgccgccct tcaaggtcag cttccgcgtg
     1021 gcgccctttt cgttgccgcc ctggccctcc gtcttctcaa tcgcgggatg ccaggacatg
     1081 tcctggaaat tgccgatctt cgcccagacc ttttcggccg gagcattgat ttcgacggac
     1141 tcgatgagtt tctgacggct tgggccatgc gccatcgcaa gcgttgggaa tgcgagaagt
     1201 gcggccgcaa caagggagcc ggtcatacga agcgtgagag tggtctgcat gtaggggttt
     1261 ccgtttcctg cccatgaaga ggtcttaagc tttcgcacag gaaatccctc ctgcgcactt
     1321 aatcaaggca ccttggactt aagaactaag acaaaccgtc ggagcatccc ctcatatcac
     1381 cacttggagc ttttccaggc aggtcatcct gtaaggttta caaagagcta agattggttt
     1441 tgttttgtaa acaccccata agtaattata caaataattt tatttctggc caccgagaag
     1501 ggcgatgggc gtcgtgactt atggcggtcc gtataactca gccgcaacgg caagactgaa
     1561 atcgcgtcca gccgggaagc aactgtggaa gtgcaaacac cgcatttggc ctatctccaa
     1621 tttgagcaaa atcagatctg taagtgaaag tattaataaa aggtttgatc attaactacc
     1681 ccgtcaattt gttattcccc aggggtttgt taagtgtaga tttagtatgt gtaacgtatt
     1741 tgagtgtgtg tcatgacccg gcgtacgttt agaccgcctt gaggcagcag acaaaaaact
     1801 tttgggcttg gtattcgccc cgatatcagg gggcggctga aaatgggcat gtgattgcac
     1861 ctgggtctgt tatgtcgata gaaaacttca gtgcggtgtt gggcaagctt tcggaaaacg
     1921 ggggaaatcc tgagctggat ggacctgctt tccgggtttc cacaaaggtg gccgaagtca
     1981 tgctttcgct tggtgcggcc atcgcaagga atgcagtgcg tgcctctccc tattatgagg
     2041 agacgctaga cagtgcaccc gtgccggctc cactctccct ttcggagctg aaggagcagg
     2101 ttaccagggc cacatccatc gctgagctcc gcggcctgag acggcgcttc gcgcgttgca
     2161 gccacccgga tcgccacaac gatagcgaag attccaccgc tacctccgaa atggcggcgg
     2221 ccaatcagtt gatcgataaa gcgatcagct caatgcgctg cccgcgctga cgcctatctg
     2281 cacatggggg catttgcgag tcgttaagta cacttgccaa caaattactt caagctccag
     2341 cgggagcgct aactccggct ccatagcctt atgccagact atggtttgtt attcgggata
     2401 gagtgggttg gcctcagggg cgtagcgtat gtttcacagg tgcgttctag acggagacct
     2461 caacgcaagt ttactccagg atgtctggtc caacgtcgag aagctctcgg aagcaagtga
     2521 ggatgtcatc ctcgacctct ctcgcgtaaa tttcatggat agccatggcg taggcgcagt
     2581 ggtggcccta atgcggcggc tgggcctcaa agggcttcag ctgaaggtca tcggtttgca
     2641 cgggcagccg ttgcggctgt tcctggactt acatctcgta ccggtctcgg gagggtgcta
     2701 acgatcgttt ctgctaagca aactccgcta atcaagccaa cggattat
//
LOCUS       DATMGX010000852         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_255797, whole genome shotgun sequence.
ACCESSION   DATMGX010000852 DATMGX010000000
VERSION     DATMGX010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255797"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..477
                     /locus_tag="VNR65_13915"
     CDS             259..477
                     /locus_tag="VNR65_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWJ19814.1"
                     /translation="MPVTNPLKCILSERDKEVLLHIADGLSDREIAAKLNLSPKTVNF
                     RVEEMKRKLDAKTRVQVVAIALRQGWID"
     gene            complement(474..1478)
                     /locus_tag="VNR65_13920"
     CDS             complement(474..1478)
                     /locus_tag="VNR65_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19815.1"
                     /translation="MSALLPLYYPTTCLVIDDDRLFLDSFYYNYADVMLCATEQRPEQ
                     AIMRLLKDAERTGLTIEEANLAPVDEGEASDPIIRLPASRIAAMARDPARFSHVSVVV
                     VDFAMPSMTGVEFLQKVKHLPVKKVLLTGKAGDSTAVAAFNEGLINLFLVKQDPDLPD
                     KLKRIIRELQYGYFKDITAPLEPIAKLEDMAFLDDPGIASWYHKLAGRIRAVEHYLLP
                     SPPGLMLVDEAGQVTIAWINNQDRMRAQLEIAIESGAPAALVKELRSGDVILTCPTPT
                     GFYKEQHMSNWQKYVLPCEEIRGNTLWKAAIQRDQALSLSAVSLTQFRRSQAGHIYPT
                     "
     gene            1601..>2894
                     /locus_tag="VNR65_13925"
     CDS             1601..>2894
                     /locus_tag="VNR65_13925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWJ19816.1"
                     /translation="MTGDQRPKGRALLGRLRDLVRRLRTAYVEYHAVAAFKLPALGLL
                     AVIAFPLYYFIWTYAFPQPYESFPLRLAGTVLCVPVALQRHWPQRLVSYYLTYCYWAI
                     LFAGPAFFTLMLLMNRANSVWLMSETVIIMFTFLLYDLANGVLVSVLGALIGLIGYWL
                     LSGEANVPPEFLLALPIYGFILSAVVFLSHSERVIAREKLMAARALASSIAHEMRTPL
                     LGIRLDASKTGDHLGRLSAANQWARERGCEGSLSDRDMAGLSGALKRIAKHAVAANLV
                     IDMLLTNLKDEGYSQEREEFYAIASTIDEAISRFQFRPGERELIRVRVYDNFIYRGVE
                     VLMVHVIFNLVKNALRAIAAAGEGQILIEARTTPGGHLLSISDTGRGIEPAILPYIFV
                     PFTTAHAETGGTGIGLSFCRRVVEGFGGSIFCSSEPSKG"
BASE COUNT          577 a          831 c          844 g          642 t
ORIGIN      
        1 gttcgcggca tcgaggctcg cgccgaatac agatcctgcg ctacctgcat tcgttagatt
       61 tcgctctgca cggccacaac gcgagctatg tccaattatg gcacttgccc tgcattatgg
      121 cgtacgcacc cggctcttgg actggtcgag aagtctgtac gtagctgctt actttgcggt
      181 aacgtaagct atgcgtattt atacaagcaa ttctgacgct aaggagccaa agctggccgt
      241 ttggaagggc gaggccatat gccagtgacc aaccctctaa agtgtattct gagcgagcgc
      301 gacaaggagg ttctgttgca tatcgcggac gggctcagcg accgggagat cgcggcgaag
      361 ctcaatctca gcccgaagac ggtcaacttc cgtgtggagg agatgaaacg caagcttgac
      421 gccaagacgc gcgttcaggt cgtggcgatc gcgttgcgcc agggctggat agactaagtt
      481 gggtagatgt gcccggcctg tgaccgacga aattgcgtca gcgagacagc cgacaagcta
      541 agtgcctggt cccgctggat cgccgctttc cagagagtgt ttcccctgat ctcctcgcac
      601 ggcaaaacgt acttctgcca attggacatg tgctgctctt tgtagaaccc ggtcggcgtt
      661 gggcaggtga gtattacgtc gcccgagcgt agctctttca caagcgccgc gggagcgcca
      721 gattcaatcg caatctcgag ttgggcgcgc atccgatctt ggttgttgat ccatgcgatg
      781 gtgacctgac ccgcctcgtc gaccagcatc aggcctggcg gagatggcag gagatagtgc
      841 tcgaccgcac gaatgcgccc ggcgagcttg tggtaccaac tcgcaatccc tggatcgtcg
      901 aggaacgcca tgtcctcaag ctttgcgatg ggctcgagcg gggcggtgat gtccttgaag
      961 tagccgtact gaagctcccg gatgatccgc ttcagtttgt cgggtaggtc tgggtcttgc
     1021 ttgaccagga acagattaat caagccctcg ttgaaggcgg cgacggccgt cgaatccccg
     1081 gccttgccgg taagcagcac cttcttgacc ggcaggtgct tgaccttctg gaggaactcg
     1141 acacctgtca tactcggcat ggcaaagtcg accacgacca cggaaacgtg tgaaaagcga
     1201 gcgggatcgc gggccattgc agcgatgcgc gatgccggca ggcgaataat aggatcgctg
     1261 gcctcccctt cgtcaaccgg agcaagatta gcttcttcga tcgtcaagcc ggttcgctct
     1321 gcatccttta gaaggcgcat aatggcctgc tccggccgct gctcggttgc acacagcatc
     1381 acatcggcat aattgtagta gaagctgtcg aggaagagcc ggtcatcatc aatgacgagg
     1441 catgtcgtcg ggtaatagag gggcagcaag gcgctcataa gtccggtcct gatcctcgcc
     1501 agggcgctcg taaggcactt ccggcagctt gcgcaaacct ttataattag cgcacgtgat
     1561 atcgtgcaag tgaaagtttg tagtcgtgcg acttggtata atgaccggcg accaacgccc
     1621 gaaggggcgg gccttactcg gccgcctgcg ggatcttgtg cgccgtcttc gcaccgccta
     1681 tgtcgaatac catgccgtcg cagcattcaa gctgccggcc ctggggctgc tcgcggttat
     1741 tgccttccca ttgtattatt tcatctggac atatgctttt ccgcaacctt acgagagctt
     1801 tccgctaagg cttgcaggaa cggtgctctg cgtgccggtg gcccttcagc gccattggcc
     1861 ccagcggctc gtatcatatt acctgaccta ctgttattgg gccatcctat tcgcagggcc
     1921 agcgtttttt acgctgatgt tgctcatgaa ccgtgccaat agcgtctggt tgatgtcgga
     1981 gaccgtgata atcatgttca cgtttctgct ctacgatctc gccaacggcg ttcttgtctc
     2041 ggtcctggga gcgctgatcg ggctgatcgg ctattggctc ctgtccgggg aggccaatgt
     2101 tcctcccgaa tttctgcttg cgctgcctat ttacgggttc atcctcagtg cggtggtttt
     2161 cctctcgcac agcgagcgcg tgatcgcgcg tgagaagctc atggccgcac gcgctcttgc
     2221 gagcagcatc gcgcatgaga tgcgcacgcc ccttcttggc atccggctcg acgctagcaa
     2281 aaccggcgac caccttggac ggctgagcgc ggccaaccaa tgggcgcgag agcgtggctg
     2341 cgaaggcagc ctcagcgatc gggatatggc tggacttagc ggtgctctaa aacgcatagc
     2401 gaaacacgct gtcgccgcga acctcgtcat cgatatgctc ctgaccaacc tcaaggatga
     2461 gggctattcc caagagaggg aagagttcta tgccatcgcc agcaccattg atgaggcaat
     2521 cagccgcttt caattccgcc caggcgagcg cgagttgatc agggttcgcg tctacgacaa
     2581 cttcatctat cggggtgtcg aggtgctaat ggttcacgtc attttcaacc tcgtcaaaaa
     2641 cgcgctccgt gcgatcgctg cggcaggaga agggcaaatc ctcatagagg cgagaacgac
     2701 gccgggtggt cacttactct cgatcagcga caccggccgc ggcatcgaac ccgcgatatt
     2761 accctatatc ttcgtaccgt tcacgaccgc tcacgcggaa accggcggca ccggcatcgg
     2821 tctgtccttc tgccgccgtg tcgtcgaggg tttcggcggc agcatcttct gctcatcgga
     2881 gcctagcaaa ggca
//
LOCUS       DATMGX010000853         3088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_143606, whole genome shotgun sequence.
ACCESSION   DATMGX010000853 DATMGX010000000
VERSION     DATMGX010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143606"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VNR65_13930"
     CDS             complement(<1..311)
                     /locus_tag="VNR65_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19817.1"
                     /translation="MYPAIVFLPLAGAVIAGFFGRTLGDKTSGFLTSLFLVAAAGLSW
                     IAFYHVGFLGEEARVPLFPFIGVGDLQTSWSLRIDTLTAVMLVVVTTVSSLVHIYSVG
                     Y"
     gene            complement(321..662)
                     /gene="nuoK"
                     /locus_tag="VNR65_13935"
     CDS             complement(321..662)
                     /gene="nuoK"
                     /locus_tag="VNR65_13935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWJ19818.1"
                     /translation="MPNTISPIEAATGIALQQYLTVAAILFTLGAFGIFLNRKNVIII
                     LMSIELMLLAVNINLVAFSAYLNDITGQIYALIVLNVAAAEAAIGLAIIVVYYRNRGS
                     IAVDDINSLRG"
     gene            complement(668..1270)
                     /locus_tag="VNR65_13940"
     CDS             complement(668..1270)
                     /locus_tag="VNR65_13940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005164.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWJ19819.1"
                     /translation="MTIAGFFFYLFSIVLLGSGAMVITARNPVHSVLFLILAFFNAAG
                     LFVLLGAEFLAMILVIVYVGAVAVLFLFVVMTLDIDFSELRAGTGRYLAVGLGIGLIL
                     LTELILMVGGFAVAPGAVQPIPAEQVTNAAAIGRVLYTKYVYFFELSGGVLLVAMIGT
                     IVLTLRHRVGAKRQDVAEQVGRRRQDAIEVVKVQTGKGIS"
     gene            complement(1556..2047)
                     /gene="nuoI"
                     /locus_tag="VNR65_13945"
     CDS             complement(1556..2047)
                     /gene="nuoI"
                     /locus_tag="VNR65_13945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWJ19820.1"
                     /translation="MGGFGQSLQSAVLKEFVAAFWLGMKYFFKPKATINYPYEKNPVS
                     PRFRGEHALRRYPNGMERCIACKLCEAICPAQAITIEAGPRRNDGTRRTTRYDIDMVK
                     CIYCGYCQEACPVDAIVQGPNFEFATETREELLYDKEKLLANGDRWERVLAMNLKLDA
                     PYR"
     gene            complement(2079..3059)
                     /gene="nuoH"
                     /locus_tag="VNR65_13950"
     CDS             complement(2079..3059)
                     /gene="nuoH"
                     /locus_tag="VNR65_13950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWJ19821.1"
                     /translation="MLGQSLLTLVALLLFIAYILLADRKIWAAVQLRRGPNVVGAFGL
                     LQSFADFLKFVLKEPIIPAGSDKIVFLLAPMVTAVLALSAWAVIPVADGWVVSDLNVG
                     ILYIFAISSLGVYGIIMGGWASNSKYPFLGALRSAAQMVSYEVSIGFVMITVLLCAGS
                     LNLTEIVKAQHGPYGLFSWYWLPLFPMFIIFFISALAETNRPPFDLPEAESELVAGFM
                     VEYSSTPYLLFMLGEYVAITVMCALITILFLGGWLPPVDVAPFTWVPGIIWFILKFCF
                     VFFMFAMVKAIVPRYRYDQLMRLGWKVFLPTSLAMVVIVAAVLQFGGLAR"
BASE COUNT          771 a          906 c          870 g          541 t
ORIGIN      
        1 atatagccga cggagtagat atggacgaga gaggagaccg tggttaccac caccagcata
       61 acagccgtga gtgtatcgat gcgcagcgac cacgaggtct gcagatctcc gacgcctatg
      121 aagggaaaaa gaggaacacg agcctcctcg cctaaaaagc cgacgtggta gaaagcgatc
      181 caggacagcc cagcagcggc gacgaggaaa aggctcgtca aaaatccgga ggttttatcg
      241 ccaagcgtgc gcccgaaaaa gcctgcgatg accgcgccgg ccaagggcaa gaatacaatc
      301 gctggataca ttcgcgcgcc tcaccctctc agcgagttga tatcatcgac ggcgatggag
      361 ccgcggttcc ggtagtaaac gacgatgatg gcaagcccta tggccgcctc ggccgcggcg
      421 acgttcagca caatcagcgc gtaaatctgc cctgtgatgt cgttcaggta ggccgagaag
      481 gccacgaggt tgatgttcac ggcgagaagc atcaactcga tcgacatcag gatgatgatg
      541 acgttcttgc ggttgaggaa aattccgaac gcgccaagcg tgaacaggat cgccgccacc
      601 gtcagatatt gttggagcgc gatgccggtc gctgcctcga taggagagat cgtgtttggc
      661 atgacggtca ggaaatcccc ttccctgttt ggactttaac cacttcaatt gcatcctggc
      721 gacgccggcc aacctgctcg gccacgtcct gccgcttggc gccaacgcga tggcgaagcg
      781 tcagcacaat cgtcccgatc atcgcgacga ggagcacccc acccgacagc tcgaagaaat
      841 aaacatactt cgtgtaaagc acgcgcccga tcgcagccgc gttcgttacc tgctccgcgg
      901 ggatgggttg gacagccccc ggcgctacag cgaagccacc aaccatgaga atgagctcgg
      961 tgagcaggat gaggccgatc ccaaggccca cggccagata gcgaccggtt ccggcgcgca
     1021 actccgaaaa atcgatgtcg agcgtcatca cgacgaagag gaaaagaaca gcgacggctc
     1081 cgacatagac gatcacaagg atcatcgcca gaaactccgc gcccaaaaga acgaaaagcc
     1141 ccgccgcgtt gaagaaggcg aggatcagga agagaacgga gtgaaccgga ttgcgcgcgg
     1201 tgatcaccat cgcgcccgac ccgaggagca cgattgaaaa caggtagaag aagaacccag
     1261 caattgtcat gcgcggccga tctcgttttg tgcggcgtaa accgtgtgtc ttttaagcgg
     1321 aacccctcat gcggctttct tcaagggaag gaatgccagt tcctttgtcc ctttcggatc
     1381 aacgtttcgg cgaggggagt tcttgtacgg agcccgggtc ggcccggctc ctgccccacc
     1441 ccgcccctcg tcagcgtaga gcatattttg atttccgaac ttccgcatta ccgacctcgg
     1501 caataaggac gaacgcaatt ttcaaacgga cttgttggac gaaagagccg aacgattagc
     1561 gatagggcgc atcgagtttg aggttcattg cgagaacgcg ctcccacctg tcgccgtttg
     1621 ccagcagttt ctccttatcg taaagcagtt cctcccgcgt ctcggtcgcg aactcaaagt
     1681 tcggcccttg gacgatggca tccaccgggc atgcctcctg gcaatagccg caatagatgc
     1741 atttgaccat atcaatgtcg tagcgcgtcg tgcgccgcgt cccgtcattg cggcgcggtc
     1801 cggcttcgat cgtaatcgcc tgagccgggc agatcgcttc gcaaagctta cacgcgatgc
     1861 agcgctccat cccgttggga tagcggcgca atgcatgctc tccgcgaaag cgcggactga
     1921 cggggttttt ctcgtacgga tagtttattg tcgccttcgg cttgaagaaa tacttcatcc
     1981 caagccagaa cgcggcaacg aactccttca aaaccgccga ttgaagagat tggccgaagc
     2041 ctcccattca tgcactcctt gacgaaaaag ctttgacgct agcgcgccaa gcctccgaat
     2101 tgtagaaccg ccgccactat caccaccatc gcgagcgacg ttggcaggaa aactttccag
     2161 cccagccgca tcagctgatc gtagcggtag cgcggcacga tcgccttcac catcgcgaac
     2221 atgaagaaga cgaagcagaa tttcagaatg aaccagatga tcccgggcac ccacgtgaag
     2281 ggcgctacgt cgacgggcgg aagccatccg ccgaggaaga gaatagtgat caatgcgcac
     2341 atcacggtga tcgcgacata ctcgccgagc atgaagagca agtaaggcgt cgacgaatat
     2401 tcgaccatga agcccgcgac cagttccgac tctgcttcag gcaggtcgaa cgggggcctg
     2461 ttggtttccg ccagcgccga tatgaagaat atgatgaaca tcggaaaaag cggcaaccag
     2521 taccagctga aaagcccata cggcccgtgc tgcgctttca caatttccgt caggtttagc
     2581 gaccccgcgc agagaagcac cgtaatcatc acaaagccaa tcgagacttc gtatgacacc
     2641 atttgcgccg ccgagcgcag cgctccaagg aagggatatt tcgagttcga cgcccaaccg
     2701 cccatgatga tgccgtacac gccgagcgag gaaatggcga aaatatagag gatgccgacg
     2761 ttcagatcgg atacgaccca cccgtccgcg accggaatca ccgcccaggc tgaaagcgcc
     2821 aataccgctg tcaccattgg cgcaagcaaa aagacgatct tgtcagagcc cgcgggaata
     2881 atgggttctt tgagcacgaa cttgaggaag tccgcgaagg actgaaggag gccgaacgcg
     2941 cccacaacat tcgggccgcg ccgcaactgg acggccgccc aaatcttccg gtcggcgaga
     3001 aggatgtagg caatgaacag gagcagcgcg acgagcgtca gcaggctctg tcccagaatg
     3061 agcgccgtcg ggaagccata cttgcccc
//
LOCUS       DATMGX010000854         3130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_24940, whole genome shotgun sequence.
ACCESSION   DATMGX010000854 DATMGX010000000
VERSION     DATMGX010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24940"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..376)
                     /locus_tag="VNR65_13955"
     CDS             complement(35..376)
                     /locus_tag="VNR65_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19822.1"
                     /translation="MNFNENFVNGSDSSQGVHKGRLFDREDRESYFRPKSENARRNEK
                     PASSTLDWVSPAAKEPNEREQERLQRLEREFVDRLIENYERAVEDGLPPNTAIASMLE
                     WVSRECPRLLP"
     gene            complement(549..1274)
                     /locus_tag="VNR65_13960"
     CDS             complement(549..1274)
                     /locus_tag="VNR65_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ19823.1"
                     /translation="MPDPIYIAVVDDESEVRHVLRQGLEAEGFAVVEASDKASCLLSL
                     EIYPIKLITLDLKLDHQDGLGLAREIRASRNVPIIMITGCDTPLDRVAGLELGADDYI
                     TKPFHIKEVVIRVRRVLATYGVLDPAPPMVSNPSCYAFDSFLFEARKQELRFASGPLI
                     ELTETEVRLLELFLTYPGRVLSRDEISQMLRGHEWSPLDRIIDGHVSRLRRKIDSPGK
                     EETSLIKSVRGVGYVFAAEVRHV"
     gene            complement(1557..>3130)
                     /locus_tag="VNR65_13965"
     CDS             complement(1557..>3130)
                     /locus_tag="VNR65_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWJ19824.1"
                     /translation="LRESEELLRAIIEHAPVPILLFREDRKILLINPALTKLTGYTAS
                     DIPTRDEWEAWAYRENAPSVKKEVREVFEQGIPTDRGALWVYTKSGEKRLWTIKTAPA
                     GCDASGKRLMVTVGLDITERKKSEDALASALRLEAVGKLAGGVAHDFNNLLAVITGNL
                     ELAQDRISDETIRDPIRRALNAGEKGSDLNRRLLSLARKRTLKPEHLNLNTRVEDITK
                     LLEATLSEHIEVATELSADLWMTLADPGEIDSAILNIAANARDAMPGGGRLRISTGNV
                     TVDASAAARLHPDSRPGDYVQLIIADNGVGMSKDVLRKAIEPFFTTKEPGAGTGLGLA
                     SVASFARHTGGFAAVESASGCGCAVSLFLPRSVEAPPAPKIVEDRLPLGDGELVLVVE
                     DDDQVREITLKRIESLGYSVTEARTGAEAVQQLKSEEPVQIVLSDVVMPGAMTGYDVA
                     RWVASNKPEINVILCSGYNEEDPRGDSQSSIRGIVVLGKPYSRAQLARALSDAVSPPK
                     ILQSEWIKGEDTDGF"
BASE COUNT          645 a          913 c          854 g          718 t
ORIGIN      
        1 ttattgccgt caaggtgatg cgggcgtttc ccgctcaagg gagcaaacgc gggcactcac
       61 gggacaccca ctccagcata ctggctatag ctgtattcgg aggaagcccg tcctccaccg
      121 cgcgctcata gttctcgata agccggtcga caaactccct ttccaacctt tggagccgtt
      181 cctgctcccg ctcgttcggt tccttggccg ctgggctcac ccaatcgaga gttgatgacg
      241 ccggtttctc gttccgacgg gcgttctccg atttcgggcg aaaataggac tccctgtcct
      301 ctctatcgaa aagccgccct ttgtgcacac cttgcgaaga atctgaacca ttgacgaaat
      361 tttcgttgaa gttcattgcc tgggcccttc tctaccgcgt gcaagagcgg cctatatcgc
      421 ccagccaacg acggcccgaa agattagaat tgtgcagaat tgtgtatcag ttgttgcgat
      481 agtgaatgca aggaatcgct cgacaccaga cgtcaacgcg tgcgcataag tgcttcaaaa
      541 cgacggcatt aaacatgcct gacttcagcg gcgaagacat accccacgcc tcggacgctc
      601 ttgattaaac tcgtttcctc tttcccggga ctgtcgatct ttcggcgcag ccgcgagacg
      661 tggccgtcga ttattcgatc gagcggagac cactcatgcc cgcgcagcat ctggctaatc
      721 tcatcgcggg agagaacgcg gccggggtat gtcagaaaca actcaaggag gcgcacctct
      781 gtttcggtca gctcgataag agggccgctg gcgaacctta attcctgctt gcgagcttca
      841 aaaaggaaag aatcgaaagc gtagcatgaa gggttgctca ccattggcgg cgccggatcc
      901 agaacgccat aggtggcgag cacacgccgg accctgataa cgacctcctt gatatgaaac
      961 ggctttgtaa tgtagtcgtc ggcgcccagc tcgagcccgg caacccggtc aagcggagta
     1021 tcacagcccg taatcatgat gatcggcacg ttgcgagatg ctctgatctc tcgggcaagc
     1081 ccgagcccgt cttgatgatc aagcttcaga tcgagggtga tcagcttgat cggatatatc
     1141 tcaaggctca gcaagcaaga agctttgtcg ctcgcctcga ccacggcgaa gccctccgcc
     1201 tcgagacctt gccgcagcac atggcggact tccgattcgt catcgacaac tgctatgtag
     1261 atcggatcgg gcaaatcctc taacctctag cagaagctcc agcccagagc tttgaacaag
     1321 gcttagcgcc tgggagcata gtgtcccgcc ccacggcttg cctgggtttc tttataattt
     1381 ttctgtcacc gatttgctac tattatatcg gattttttga tcgatccttc gtgttttgtc
     1441 aaaggttaag gaaatgaact tcaagctgac gcactccctg agggtggcgg gccactctaa
     1501 gcgctctcct ggagcagccg gcgtccgttg gtagcggata agctgctctc tagactttaa
     1561 aatccatccg tgtcttcgcc tttaatccat tccgattgaa ggattttggg tggcgagaca
     1621 gcatcgctca aagcccgcgc gagttgggca cggctatatg gcttgccaag aaccacaata
     1681 ccccggatgg aactttggct atcacctcgg gggtcttctt cattgtagcc agagcataat
     1741 atgacgttga tttcgggctt atttgacgcc acccagcggg ccacgtcgta gcccgtcatg
     1801 gcgccgggca tgacgacgtc gctcagcaca atttggaccg gctcctcgga ttttagctgc
     1861 tggacagcct ccgcacccgt tctcgcttcg gtgacggaat agcctaagga ttcgatccgt
     1921 ttgagcgtaa tctcgcgaac ctgatcgtcg tcctcaacca cgagcacaag ttcgccatca
     1981 ccgagcggaa gccggtcttc gacgatctta ggcgcgggcg gagcttcgac cgagcgcggg
     2041 agaaaaagac ttacggcaca accacatccg gaagcacttt ccaccgcggc gaatccgcct
     2101 gtgtgcctgg caaagcttgc aacgctggcg agtccgagtc ccgtccccgc gcctggctcc
     2161 ttcgtggtaa aaaagggctc tatcgccttt cggagcacgt ccttggacat tccgacgccg
     2221 ttatcggcga taatcaactg aacgtagtct cctggccggg aatcaggatg aagcctggcg
     2281 gcggccgatg catcgacggt gacgtttccc gtggagatcc gaagcctgcc cccgcccggc
     2341 atcgcatcgc gtgcgttcgc ggcgatgttc aatatggcgc tgtcgatttc gcccggatcg
     2401 gccagcgtca tccagaggtc agcactgagt tccgtggcga cttcgatgtg ttcgctcagg
     2461 gtcgcttcaa gaagcttggt aatgtcctcg acgcgggtgt tcagattgag atgctccggc
     2521 ttaagagtgc gcttacgcgc cagcgaaagc agccgacggt tcaggtcgct gcccttctcg
     2581 ccggcattta aagcacgccg gatgggatcg cggatcgttt catcactgat ccggtcttgg
     2641 gcaagttcaa gattgccggt aattacggcc agaaggttat tgaaatcgtg cgcgacgccg
     2701 cccgcaagtt tgcccacggc ttctaatctg agggcgcttg cgagagcatc ctcgcttttc
     2761 tttctttcgg tgatgtcgag tccgacggta accataaggc gcttaccgga ggcatcacat
     2821 ccggctggcg ctgtcttgat cgtccaaagg cgcttttcgc cggatttcgt gtagacccaa
     2881 agagcgccgc gatctgtagg aatgccttgc tcgaaaactt cgcgcacttc cttcttcacc
     2941 gacggtgcat tttcacggta ggcccacgcc tcccactcat ccctggtcgg tatatcggac
     3001 gctgtgtagc ccgtgagttt cgtcagagcc gggttgatga gcaggatctt ccgatcctct
     3061 ctgaacaaga gaattggaac gggcgcgtgc tcaatgatgg cgcgcaatag ctcttcgctt
     3121 tcgcgcagcg
//
LOCUS       DATMGX010000855         4743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_8725,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000855 DATMGX010000000
VERSION     DATMGX010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8725"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1005
                     /locus_tag="VNR65_13970"
     CDS             136..1005
                     /locus_tag="VNR65_13970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ19825.1"
                     /translation="MSARFEPIIGRYMHLDLFGRKHRIYVEQAGEGTPLLCLHTAGAD
                     GRQYRALMNEARVTQRHRVIAFDMPWHGKSSPPSGWHNEEYQLTSAQYTTMVLEIMTA
                     LELDRPILIGCSIGGRIALHLALEHPERFRAIIGLQAGAHVDPYYDLNFLHRPDVHGG
                     EVSAAIVSGLVGPDAPDSERWEALWHYMQGGPGVFKGDLYFYKLDGDIRGRVAQIDTR
                     CCPLFLLSGEYDYSCTPEETLAVARSVPGSEAVIMKGLGHFPMSENPTEFMEHLLPVL
                     EKIIALEAAVAAK"
     gene            2099..2236
                     /locus_tag="VNR65_13975"
     CDS             2099..2236
                     /locus_tag="VNR65_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19826.1"
                     /translation="MILARIEPAKPGFDLRTFECSKCNNADQYIIEYGTAAPWALHVR
                     A"
     gene            2242..2430
                     /locus_tag="VNR65_13980"
     CDS             2242..2430
                     /locus_tag="VNR65_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19827.1"
                     /translation="MSGRNSHDWDARASDALAEALAMPKGADRSDALKKAGRLRVAAD
                     MQQNLRSQTVGRSGRNAP"
     gene            3078..3362
                     /locus_tag="VNR65_13985"
     CDS             3078..3362
                     /locus_tag="VNR65_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19828.1"
                     /translation="MDNKLNEIRRKIQLLRSEMLTAGKSIRNQISQDEDCTEASLRLM
                     AMRNIMLGLIHERDSLGGSERLLDVEERLKADYRAARRKPLKGALGRRER"
     gene            3352..3534
                     /locus_tag="VNR65_13990"
     CDS             3352..3534
                     /locus_tag="VNR65_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19829.1"
                     /translation="MNDEPLAWKIAALRGQLLELGSTIRQLNQSGLDSATAQLLLARK
                     RAELDDLMKKGRSNAS"
     gene            3524..3754
                     /locus_tag="VNR65_13995"
     CDS             3524..3754
                     /locus_tag="VNR65_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19830.1"
                     /translation="MPHEPAKWYDHNTDNPPWIRALAEVRLLAPREGYCFHHVQAIIV
                     AIDQYAEKALGNREFFLNKPYGIGGNRKDNTP"
     gene            complement(4057..4473)
                     /locus_tag="VNR65_14000"
     CDS             complement(4057..4473)
                     /locus_tag="VNR65_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HWJ19831.1"
                     /translation="MIFGLRRRRLLVLGLLFTMTLAGCASWITYGDGDLKGHVVVVWN
                     RQDKFIYVPDNNDLFSFQPSFMKATNTRIVPGLMYTDGGSIPRVFWSVPGLSPWAFGP
                     AYIVHDWLFVVHRCHWPAPPEVQAITSSSRLRSWPK"
     gene            complement(4544..>4743)
                     /locus_tag="VNR65_14005"
     CDS             complement(4544..>4743)
                     /locus_tag="VNR65_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19832.1"
                     /translation="GKWPGINRVSGLLSGADIAGLIAKPDGNADRWAGRVDHARLNTR
                     ATCAGRHLPLPREVENEADAL"
BASE COUNT         1016 a         1419 c         1408 g          900 t
ORIGIN      
        1 gcccggatcc aacgacctga tggcgctgat ccggcggcgc gtattgaagg ccgagggcga
       61 cctgcagatc ttcatggcca acctgcgcta cttcaaggag atgctggcaa aacttcgcac
      121 ccgggcagag gcggcatgag cgcgcgcttc gagccgatca tcggccgcta catgcatctc
      181 gacctgttcg ggcgcaaaca ccgcatctat gtcgagcaag ccggcgaggg cacaccactc
      241 ctgtgtctcc acaccgccgg cgccgacggg cgccaatacc gggcgctgat gaacgaggcg
      301 cgcgtcaccc agcgccatcg cgtcatcgcc ttcgacatgc cctggcacgg caaatcctcg
      361 ccgccttcag gctggcacaa cgaggaatac cagctcacct cggcgcaata cacgacgatg
      421 gtcctggaga tcatgaccgc gctcgaactc gaccggccga tcctgatcgg ctgttcgatc
      481 ggcggccgca tcgcgttgca tctggcgctc gaacaccccg aacgctttcg cgccatcatc
      541 ggcctgcagg ccggcgcgca tgtcgatccc tattacgatc tgaatttcct gcaccggccc
      601 gacgttcatg gcggcgaggt ctccgcggcc atcgtctccg gactggtcgg gcccgatgcg
      661 ccggacagcg agcgctggga agcgctgtgg cactacatgc agggcggtcc cggcgtcttc
      721 aagggtgacc tgtatttcta caagctcgac ggcgacatcc gcggccgcgt cgcgcagatc
      781 gacacacgct gctgcccgct gtttctcttg tcgggggaat acgattactc ctgcacgccg
      841 gaggagacgc tcgcggtcgc caggagcgtg cccggctcgg aggcggtcat catgaagggc
      901 ctcggccatt tcccgatgag cgagaacccg acggagttta tggagcacct cctgccggtg
      961 ctggagaaga tcatagccct cgaagcggcg gttgcggcga agtaacgcgc gagacttcga
     1021 gcgggtcacc aaaatcaacg gggatagcgt cagctaagca tcctgcaagg cagaccagat
     1081 cgcgccaatc gcaccaaatc tcggtatgtc gcgattcgtt tcgggtggac tttattgctc
     1141 gccgcgtatc gagatatcct cccttaactg cccggtggcc tcgaagctgc cgggtttttt
     1201 tgcgttgacg tcgtcccgta aaactacggt gttctggcca cagccgcgcc caaatctttt
     1261 cgcgtgtata atatcgaaca gacaacctgt atcttagtag ctgattgccg tcgcctcatg
     1321 cttctggcgg agagcggagc cgaagccacc attctgacga gcctcacccg aagctggaag
     1381 atgatcgcca accagaccga ccgatatgtc gcgatcatga aaagggagac cgctcgaaaa
     1441 ggcaggcccg cccgatagtc gggacaactc gacttagcaa gtttcggaat gtgagcaata
     1501 ttgactagcg actccgggaa cctgaaaggc attctggctc gatcaccgtc cccgcgctcg
     1561 gcatctaccc ggtgactgag agttctgcgc tctcgcctga tttagggtgc atgcgatgga
     1621 cggtcctaaa tctacagccg caacaccgcc gatcgtcgtt gagagcgatc catgtccgaa
     1681 atgccagggc tcgatgatgc tggtccgtat caagccggga cgcctgaatt ttgacctgcg
     1741 cacattcgag tgcgtcaagt gcaaccacgt tgaaaaaacg ctggtgggga ccgatccgat
     1801 cagatcagcc gatgcactgg gatggctctt gagcgagctt aggccgccga cgtaagcgcg
     1861 ctgacgttgg atcgtccatc aaggcgctcc gggttgttcg tcgtcgcccg attccggcga
     1921 gctttttagg cccggatgag caatattgac cgataaccgg cggcgcgatg ggtgcaagta
     1981 acgctatcgc gtcaacggga aaggggggcc gatgtctgca agccccagaa ctcaagcaaa
     2041 ttcgctgggc gaaatcatcg aatccaggcc aaagccctgt accaagtgcg gggctccgat
     2101 gatcctggca cgcatcgagc ctgccaaacc cgggttcgat ctgcgaacct tcgaatgctc
     2161 gaagtgcaac aacgccgatc aatacatcat cgagtacgga acggccgcgc cctgggcgct
     2221 gcacgttcgg gcgtaaccgc catgtcaggc cggaatagtc acgattggga cgcacgcgcg
     2281 agcgacgcgc tggcggaggc tttggcgatg cccaaaggtg cggatcgaag cgacgcgctg
     2341 aagaaagcgg gcaggctgcg tgtcgccgcc gacatgcaac agaaccttcg atcgcagacg
     2401 gtggggcgct caggccggaa cgcgccgtag agcgcgagat cgctcgatac gtcgcagacg
     2461 acatcgcctt ggaggaagcc cgagccttga aagctcgcca acgcgttgcc gcggtcttcc
     2521 cgcaacgccg atctagctga gtgcactgac cgcccgggtc acgagatgcc aggatagccg
     2581 gccgagaaac cagagccatc cgcccatggc gaacaccacc atcaaggcaa aaagcgacaa
     2641 gacggcgcga ccccgaagca gggatcgagg accgttctga ttccggagcc gttggtcttc
     2701 ggtttcgctc atgctcgcag agtcatcgat cgttaccttg ccgtataagc gcgttcgggg
     2761 cgcttggtgt taattgaggc cggttgtctg cctgatcttg cggcttaacc taaccggtca
     2821 agcttaccgc ctgcgccgcg acagaactgt gaagtcgatc acacaaccga agtctcatca
     2881 tcacttttgg ggtcagtgga acaagcgtgc acgactgtgt tttgacaccg gcttgaaatc
     2941 caatttcctg ggaatgacgg tgtcctgtgt cgtcacggaa tttcctcggt ggcgatcatg
     3001 cgaacctgca acctgcagcc gcttgcaatt tgttcatctt ttgttcttat actaccctcc
     3061 ctgaacgagg gtgtgccatg gacaacaaac tgaacgaaat ccgtagaaaa atccaactct
     3121 taaggtcgga aatgctgacc gcggggaaga gcattcgcaa ccagatcagc caggatgagg
     3181 attgcaccga ggcctccctc cggctgatgg cgatgcgaaa catcatgctg ggtcttatcc
     3241 acgagcggga cagccttggc ggaagcgagc gcctgctcga tgtcgaggag cggctgaagg
     3301 cggactatcg cgcggcgcgc aggaagccgc tgaagggtgc gctgggccgc cgtgaacgat
     3361 gaaccgctcg cttggaagat tgcagcactg agagggcaac tgctcgaact cggctcgact
     3421 atccgacagc tcaatcaatc cggattggat agcgccacgg cgcaacttct tctcgcgcgc
     3481 aaacgggcgg agctggacga cctgatgaag aaaggccgct ccaatgcctc atgagcctgc
     3541 aaaatggtac gaccacaaca ccgacaaccc gccgtggata cgagcgctgg ccgaggtcag
     3601 gctcctggcg ccgcgggagg gctactgctt ccaccatgtc caggcgatca ttgtcgcgat
     3661 cgaccaatat gcggagaagg cgctcggcaa tcgggagttc ttcctgaaca agccctatgg
     3721 cattggcggc aacaggaagg ataatactcc ctgaggtcta cctcttcgca actcgcgcct
     3781 gaaaagaccg cctgtaccga caggcaggcg gtctttgcga ggtgggttgt agcagcgcgc
     3841 gttatggtct tcgcggcgga ggaattttga aatccacaac tgtcatcccc tggccgcccg
     3901 cgcgtagcga ttttatgttg gtcggaggcg gatgatcgca atcatgatcg ccgggaggac
     3961 tttcccaaat gtcgcgagcg tattttgtct ggatcgccca aacaattgct tcgagcttgt
     4021 tgtcatcgat taaattcgat ttgaccaatg acttgcctac ttcggccagg atctgagccg
     4081 actgctcgac gtgatggcct ggacttcggg cggagccggc caatgacaac gatgcaccac
     4141 gaacagccag tcgtggacta tgtaggcggg cccgaaagcc cagggcgaga gtcctgggac
     4201 actccagaat acgcgaggta tgctgccgcc gtcggtgtac ataaggccag gaacaatcct
     4261 ggtgttcgtg gccttcatga acgacggctg gaagctgaat aggtcgttat tgtccggaac
     4321 gtagatgaat ttgtcctgcc gattccatac aacgaccacg tgacccttca gatcgccgtc
     4381 gccgtaggtg atccaggagg cgcatccggc caaggtcatc gtgaacaata agccgagcac
     4441 caaaaggcgc cggcggcgca atccaaaaat catggcgacc cccaaagaga cacgtcgccc
     4501 gccatcccca ttgtcaggat aatgcagcct ccttaacgga catctaaagc gcgtctgctt
     4561 cgttttcgac ctcgcgcggc aagggcaagt ggcgtccagc gcaggtggcg cgcgtgttca
     4621 agcgggcatg atcaacgcgt ccagcccagc ggtccgcatt gccatcgggc ttggcgatca
     4681 aaccggcgat gtctgctcct gagagtaaac cggaaacgcg gtttatgccg ggccacttcc
     4741 ctt
//
LOCUS       DATMGX010000856         3438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_432245, whole genome shotgun sequence.
ACCESSION   DATMGX010000856 DATMGX010000000
VERSION     DATMGX010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432245"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1341..>3438
                     /locus_tag="VNR65_14010"
     CDS             1341..>3438
                     /locus_tag="VNR65_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HWJ19833.1"
                     /translation="MTEPAFGTADLTNCDREPIHIPGSIQPHGVLIVLDPDSLAVIQV
                     AGDTERLLGLAPHEMLGQGLEARIGPAALERLRTFGAQDIAIPRPILAFETAVQYHEQ
                     PLEAAVHMSGGNLVVELEPCLSEPSANPGAIAQRMIMRVHGEAELPGFFQAVANEVRA
                     ATGFDRVMVYQFQADDSGAVIAEAKAEELEPYLGLHYPASDIPKQARELYLRNWIRLI
                     PDVRYTPAPVTPALNPVTGRPLDLSFSVLRSVSPLHLEYLGNMGVVASMSLSLVVDGK
                     LWGLIACHHREPRHLRQTLRSVCELFAQMISLQLRERLTTEAQGDRLRMRRIHAELVE
                     AMVRENHLGEALIRHQPNLIDYIPADGAAVWCNGQATRLGRTPTDEQLGALVEWLNVS
                     LGEGMFMTDCLAEQFAPAKDYTDVASGVIALSASRSPRDYVFWFRPEALRTVTWAGNP
                     AKSVETGDDGVRLSPRKSFAAWKETVRGRSQPWDERIAEAARDLLISMLDVVVRHMDQ
                     VMREREQARMQQDFLMAELDHRVKNTIATIQALVRFSASGVPGLEAFTRNIQERLHAM
                     ARAHSMLTQSRWKGLDLQKVIEEQFAPFGGIDRVKITGSKLMLRPKAALSASLALHEL
                     TTNAAKYGALSVAAGWVEVSWAEQTREGKRWLALRWTEHGGPVVAPPGRSGFGRVLVE
                     RSLAYDVNGHVDLDFRP"
BASE COUNT          712 a          996 c         1018 g          712 t
ORIGIN      
        1 atcgcccgaa agccgacagc tcggtgaata attcaggcta ggaggcaaat atggatccgg
       61 gtggtttgat tgtggttaac ataatcggtc tgtcagcagt gctcatggtt gggctgtttc
      121 tttgggatgg acccagcaaa aagagatagg gtgcgcggct tgtagtggcg ccgcataggg
      181 ccggtgtggg ccttattgag gtgtggccag cgaaaactgc gtgccagtgg aaaaagaggc
      241 gccttgggct gcctgcgagc ccaagacgcc tatgcgagac tggccattga taccacgggt
      301 cttaaggggg ctacactacg gtgccgattt acacgcggta ccattttgga agaaaaaatg
      361 ccactcaagc aaacgagctc cccactctcg cggctcagtt gcaacaaaag gtgaattgcg
      421 gcgaaggcac acaagcgtta ccattattct ccttcaagga tttcttatca cggacctgcg
      481 agaccctgct caatgagcgg ggtctttggc tctttggccg atgttagcca agttattcgc
      541 gacgggtctt tcgaacgctt gatgctagcg ataacccact gcgaagctgg ccgtgttcat
      601 ccagatgacg catacattcg ccaacactct gaccagcttt aggcttaacg ccgaattgga
      661 aatcaggacg cggttcgcgt ccggacatgc tgctgtgccc ttttcactgc aaccaaaaca
      721 cggcagccaa gcaaaggctc agcttgagca ccacccacac cggtcaagcg attggcaagc
      781 gcccaaatga gccagtcttg gctcagcgcg ctcgccgtgt cgcggtttcg gacatgaata
      841 gcttaactca atattgccgg gctagagaga aggcaacgca accacatcca cagtaaaagc
      901 gcgaaattga ggatgtaatt gcttcaaaac cccacatcga gagtattaaa ttttctcatg
      961 tcgggctatc agagaccata acgaactcgt gtggtcgaca aggctggcgc ggcgattaat
     1021 cggcgatttt tccgtcgaag ggttgggacc tttaccgacc cggttcgggc acgccgtaga
     1081 acaatccgtg agggaccgct tgcgcgccgg ccgggccgac cccggcaccg ctcccaatgg
     1141 cacaaaaaaa gggagctttt tcaaggtcaa ttcttctcgc ctcgctgccg tgtcgaaggc
     1201 accgtatgcg caacctacgg cagtgagaag atttttgcag tcctgatata tctcgtctat
     1261 tgtccatccc ccatcagtcc ggagaaggtc acccgcgttt ccatcccact aatgagctcc
     1321 tccaacgaaa gtcttaggcg atgactgagc ccgcctttgg aacggcagac cttaccaatt
     1381 gcgaccggga gccgatccat atcccggggt ccatccagcc ccacggcgtt cttattgtgc
     1441 tcgatcccga cagtctcgcg gttatccagg ttgcgggcga taccgagcgt ctgctcggcc
     1501 tcgcgccgca tgagatgctt gggcaagggc tggaagcgcg tatcggcccg gctgcgctcg
     1561 agcgcttgcg cacttttggg gcgcaggaca tcgccatccc gcggcccatc ttagctttcg
     1621 aaacggccgt acaataccat gaacagccgc tcgaggccgc cgtgcacatg agcggcggca
     1681 atctggtcgt cgagctggaa ccctgcctgt ccgagccatc cgctaacccg ggggcgatag
     1741 cacagcgcat gatcatgcgt gtgcacggcg aggcggagtt gcccggcttt ttccaggcgg
     1801 tcgctaacga agtgcgtgcc gcgaccggct tcgatcgggt tatggtctat caatttcaag
     1861 cggacgatag cggggcggtg atcgccgagg ccaaagccga agagttggag ccctatctcg
     1921 ggctgcacta cccggcttcc gatattccca agcaagcccg ggaactctat ctgcgtaatt
     1981 ggatccggct cattcctgat gtgcgctaca cccccgcacc ggtcacgcct gcgttaaatc
     2041 ccgttaccgg cagacccctg gacctctcct tcagtgtttt gcgctccgtt tcgcccctgc
     2101 atctcgaata tctcggcaac atgggcgttg ttgcgtcgat gtcgctttcc ctggtggttg
     2161 acgggaagct gtggggcctg atcgcgtgtc atcatcgcga gccccggcat cttcggcaga
     2221 cgctgcgttc ggtatgcgag ctgttcgccc agatgatttc gctgcaactg cgcgaaagac
     2281 tcaccaccga agcgcagggc gatcgcctgc gaatgagacg cattcacgcg gagcttgtcg
     2341 aagcgatggt tcgcgaaaac catctcggag aagctctgat tcgacaccag cctaacctga
     2401 tcgattacat ccccgcggac ggggccgccg tgtggtgcaa cgggcaggcg accaggcttg
     2461 ggcgaacgcc cacggatgag caactcggcg cattagtcga atggctgaac gtttctttgg
     2521 gggaagggat gttcatgacg gactgcctcg cagagcagtt tgctcccgct aaagactata
     2581 ctgatgttgc aagcggtgtt attgccctct cggcatcgcg atcgccccgc gactatgtct
     2641 tctggttcag gccggaggcg ttacgcaccg tgacctgggc tggtaatcct gccaaatcgg
     2701 tcgaaaccgg cgatgacggt gtgcggctta gcccaagaaa atcttttgcg gcgtggaagg
     2761 agaccgtgcg gggacgttcg cagccatggg acgaacgtat agccgaagcg gcgcgtgact
     2821 tactcatatc catgctcgat gtggtcgtcc gtcacatgga ccaggtcatg cgcgagcgcg
     2881 agcaggctcg gatgcagcag gattttctga tggcggaact cgaccaccgg gtcaagaaca
     2941 ccatcgccac tatccaggct ctcgtgagat tttccgcgtc aggcgtcccg ggcctggagg
     3001 ccttcacacg gaacatccag gagcgcctgc acgcaatggc ccgcgcccat agcatgctta
     3061 cccagagccg ctggaagggg ttggacctgc aaaaagtgat cgaggagcaa ttcgcccctt
     3121 tcggtgggat cgatcgcgtg aagattacag gatcaaagct catgctaagg ccgaaggcag
     3181 cgctcagcgc ctctctcgct ctccacgaac tgacaacgaa tgcggccaaa tacggtgcgc
     3241 tttcggtcgc ggcgggctgg gttgaggtca gctgggcgga gcagacccgc gaaggcaagc
     3301 gctggctcgc gctgcgctgg accgagcatg gcggtccggt ggtcgcgccg ccgggccgaa
     3361 gcggctttgg acgcgttttg gtcgagcgca gtctcgctta cgacgtcaac ggccatgtcg
     3421 atctcgattt caggccgg
//
LOCUS       DATMGX010000857         6311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_458966, whole genome shotgun sequence.
ACCESSION   DATMGX010000857 DATMGX010000000
VERSION     DATMGX010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458966"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VNR65_14015"
     CDS             <1..578
                     /locus_tag="VNR65_14015"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWJ19834.1"
                     /translation="DAAIYTHDHADHTHGIDDLRTLYYRQRRRIDVWADPRTLAILRK
                     RFDYCFETPPGSSYPAIVQGHVIENHAPLTITGPGGDLVFQPFRQIHGDIDSLGLRVG
                     GVAYSCDLNDLPDEALPCLRDLDVWILNALRYAPHPSHLTMTNALAWIGRMKPKRAIL
                     THLHVDMDYDELAAQLPDGVVPAHDGLVLEF"
     gene            complement(720..1391)
                     /locus_tag="VNR65_14020"
     CDS             complement(720..1391)
                     /locus_tag="VNR65_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ19835.1"
                     /translation="MIVIVDERETVLNGYTAWFCREGVSATGLAPQDFRQWVNTAPEP
                     DIDAVEAFLLGDFQNREEVPIKIKSRSAAAVIAMNETKSLSDTLDLFAAGVDDVVRKP
                     VHVREILARIRAISRRNQQEAQQPLAGRIRVFSDGRDPEIDGEPLPLPRRERRILEYL
                     MSNKGRRVTKSQIFNFVYGLFDDGIDENVIESHISKLRKRLRQRLGTDPIDSQRFLGY
                     RLTHE"
     gene            1598..2308
                     /locus_tag="VNR65_14025"
     CDS             1598..2308
                     /locus_tag="VNR65_14025"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWJ19836.1"
                     /translation="MMRRAITQASFVGVLCFALYAPTLEARECQGQSDVPIVNLLAPP
                     PCETCAETKAEMDELAGLERERTPEQESRVAADVKRSVDRFLDGAGITFDSEKLRPCE
                     KFFTMPRQEEKAAVEAAKDTFCRTRPFKTPGNTLHPVEGARPDESFSYPSGHSAYGAT
                     IGFLLVEMLPEKRAEIYRRINDYARSRMIAGAHFRSDVEAGKLIGVAVVASLFGKPDF
                     RPAFDEARTCVRKAVGLE"
     gene            complement(2432..3322)
                     /gene="folD"
                     /locus_tag="VNR65_14030"
     CDS             complement(2432..3322)
                     /gene="folD"
                     /locus_tag="VNR65_14030"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HWJ19837.1"
                     /translation="MAEAQILNGVAMAAEIKGEVAKRAAAFASASGRQPGLAVVLAGE
                     NPASQVYVRSKLKLARELGFHSVEHRPPADISKAGLLDLIASLNADEAIDGILVQLPL
                     PAHLNADDAIAAIAPEKDVDGFHVVNAGRLFIGKPAFVPCTPLGCLRLIKSVRDSLAG
                     LNAVVVGRSNIVGKPMAHLLMAENCTVTIAHSKTQDLGAIVSSADIVVAAVGRPGMIP
                     GVWIKTGAIVIDVGINRVPALDGRTKLVGDVDFESARHVAGAITPVPGGVGPMTVAYL
                     MANTCRAAFLRHGLPWEEGV"
     gene            complement(3327..3674)
                     /locus_tag="VNR65_14035"
     CDS             complement(3327..3674)
                     /locus_tag="VNR65_14035"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HWJ19838.1"
                     /translation="MTLPATRKADGIVLYVRLTPKSSAARIGGVEDHGGKLVLKAYVT
                     TPPEDGKANAALVALVAEWLGVPKSAVTLTSGQKSRLKSVAVDGEPDKLLEKISRLLA
                     DLSQGGAQSKKRR"
     gene            complement(3802..4083)
                     /locus_tag="VNR65_14040"
     CDS             complement(3802..4083)
                     /locus_tag="VNR65_14040"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWJ19839.1"
                     /translation="MHALIGFIAMVISLYIWVIIIGAILSWLIAFDVVNRRNRAVFTI
                     ADSLYRLTEPALRPIRKVLPDLGGVDISPVILILGLIFIRDVVLLSWLI"
     gene            complement(4263..4532)
                     /gene="rpsO"
                     /locus_tag="VNR65_14045"
     CDS             complement(4263..4532)
                     /gene="rpsO"
                     /locus_tag="VNR65_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWJ19840.1"
                     /translation="MSITAERKTALIGEYGTKPGDTGSPEVQVAVLTERIVNLTEHFK
                     THKKDNHSRRGLLKLVSQRRRLLDYVKSKNEGRYRDLIARLGIRR"
     gene            complement(4567..5490)
                     /gene="truB"
                     /locus_tag="VNR65_14050"
     CDS             complement(4567..5490)
                     /gene="truB"
                     /locus_tag="VNR65_14050"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWJ19841.1"
                     /translation="MARRKGNDINGWLILDKPQGLTSNQALGRLKRLFNPKKVGHAGT
                     LDPLATGILPIAFGEATKTVAHVVDCEKSYDFTIRWGIETDTDDAEGQPTQTSDARPS
                     REAIEAALPAFIGDIMQVPPCYSAIKVEGERAYDLARDGETVELAARQVSVYELDIEE
                     AISTDLTRLICACGKGTYVRSIARDLGRALGCYGHIAALRRTSVGAFDEEAAISLEKL
                     EHISNSDGGFDALMSTIIPVETALDGIPALAVSGDDAARLKRGQSILIRGGNAPILKG
                     PIYATSRGVLIAIGEVERGEMHPIRVFNIPA"
     gene            complement(5502..5894)
                     /gene="rbfA"
                     /locus_tag="VNR65_14055"
     CDS             complement(5502..5894)
                     /gene="rbfA"
                     /locus_tag="VNR65_14055"
                     /inference="COORDINATES: protein motif:HMM:NF001802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWJ19842.1"
                     /translation="MRHQSTKGPSQRQLRAGELLRHTLAQIFQTEDIEDTELKGEIIT
                     VGEVRVSPDLKHATVYTSALSGKDSNIIAKALNRHNRFVRGELARRIELKYVPELAFK
                     GDHILDEAGRIDDLLRSPEVARDLKSET"
BASE COUNT         1253 a         1840 c         1860 g         1358 t
ORIGIN      
        1 tcgacgcagc gatctacact cacgaccacg ccgaccacac gcatgggatc gatgatctac
       61 ggacgcttta ctacaggcag cgccgccgca tcgacgtgtg ggccgatccg cgaacgctcg
      121 ccattctgcg caagcgcttc gattattgtt tcgagacgcc gccgggatcg agttaccccg
      181 cgatcgtgca aggccatgtg attgaaaatc atgcgccttt gaccatcacc gggccgggtg
      241 gcgaccttgt tttccagcca ttccggcaaa tccacggaga catcgactcg ctcggattgc
      301 gcgtcggcgg tgttgcttat tcctgcgatc tgaacgatct gccggatgaa gcgctgcctt
      361 gtttgcggga tctcgacgtc tggatcttga acgcgctgcg ctatgcgcct cacccgagcc
      421 acctcacaat gacaaatgcg ctcgcctgga tcgggcgcat gaagccgaaa cgcgcgatcc
      481 tgactcattt gcatgtggac atggattacg atgagctcgc ggcgcagctt ccggatgggg
      541 ttgtgccggc tcatgatggc ttggttctgg agttttaggg atttgtgctg cgacgcagtt
      601 aagctcggcc tcacccttgg aacaggctta gggtgaggcc gagggccgct acgaagtgac
      661 atcatggcaa gcctgtaaaa ccatgtgccc ccggttcagc ggccaaacta cgtccctgct
      721 cactcgtgcg tcagccggta gccaagaaag cgctgcgaat caatggggtc tgtgccgagc
      781 cgctggcgca ggcgcttgcg aagcttgctg atgtggctct cgataacgtt ctcgtcgata
      841 ccgtcatcga aaaggccata gacgaaattg aagatctgcg atttcgtcac gcggcggccc
      901 ttgttagaca tcagatattc gaggatccgc cgctcgcgcc tcgggagcgg caacggctcg
      961 ccatcaatct cgggatcgcg cccgtcagaa aacaccctga tcctgccggc aagcggctgc
     1021 tgggcctcct gttgattccg gcggctgatc gcgcggatgc gggcgagaat ctcgcgcaca
     1081 tgcaccggct tgcggacaac gtcatcgaca ccagctgcaa acaggtcgag cgtatcactc
     1141 aaggattttg tttcattcat cgcgatgacg gccgcagcgg atcgcgactt gattttgatc
     1201 gggacttcct cccgattctg gaagtcgcca aggaggaacg cctcaaccgc atcgatatcc
     1261 ggctccggcg cggtgttgac ccactggcgg aaatcctggg gcgcgaggcc tgttgcggag
     1321 acaccctccc ggcaaaacca tgccgtatat ccgttcaata cggtttcacg ttcgtcgacg
     1381 atgacaatca tgaggtttcc ccgaatctat ccaatactgt tcccagcaac gattaggcgt
     1441 aatgattaac ttgtgcaact ttagattgag caaaccttgt ggactaactt cgaatcgggc
     1501 tgggcgtgtc tttagggacg cgcattccgc tttgcgctgg ttttgcggga aagatgggat
     1561 cagaaagagc ctcgggcgga aggccagagg agattcaatg atgcggcggg cgattaccca
     1621 ggcgagtttt gtcggagttc tttgtttcgc gctgtatgcg cctaccctcg aagctcggga
     1681 atgccagggt caatcagatg ttccgatcgt gaatctgctg gcccctccgc cttgcgagac
     1741 ctgcgcggaa accaaagccg aaatggacga actcgcaggg ttggagcgcg agcggacccc
     1801 ggagcaggaa agccgcgttg cagccgacgt taagcgcagt gtcgaccgct tccttgatgg
     1861 agccggcatc actttcgact ccgagaagct tcgaccgtgc gagaagtttt tcacaatgcc
     1921 gcggcaggag gaaaaggctg cggtcgaggc ggcgaaagat accttctgcc gtacgcggcc
     1981 cttcaagaca cccgggaaca cgctgcatcc tgtggagggg gcacgcccgg acgagagttt
     2041 ctcctatcct tcaggacact ctgcctacgg cgctacaatc gggtttctcc tggtggagat
     2101 gcttccagaa aaaagggcgg aaatctatcg taggatcaac gactacgccc gctctcggat
     2161 gattgcgggc gctcatttcc gcagcgacgt ggaggcgggg aaactcatcg gagttgcggt
     2221 tgtggcatcg ctttttggaa agcctgattt caggccagcc ttcgacgaag ccagaacatg
     2281 cgtgcgcaag gctgtcgggc ttgaataaag cggcgaatat gccttcgtca tcccggaagg
     2341 gctgcaggcg ttgtccggga tcgtaacata tttcgagcgc actgcttttt gcgatcccga
     2401 atacggcgct aacgccgttc agggatgact atcaaacgcc ttcttcccag ggcaggccgt
     2461 ggcgcaggaa cgcggcgcgg caggtgttcg ccatcaaata ggccacggtc attggcccga
     2521 cgccgcccgg cacaggtgtg atcgcgcctg cgacatggcg ggcgctttcg aagtcgacat
     2581 cgccgacgag cttcgtcctg ccgtccagcg ccggaaccct gttgattccg acatcgatga
     2641 caatggcgcc cgttttgatc cacacgcccg gtatcatgcc aggccggccc acagcggcga
     2701 ccacgatgtc agcgcttgaa acaattgcgc cgaggtcttg ggtcttcgaa tgcgcaatcg
     2761 ttacggtgca attctcggcc atgagaagat gcgccatggg cttgccgacg atgtttgacc
     2821 gccccacgac gacggcgttc aggccggcaa gactatcacg gacgctcttg atgagccgca
     2881 ggcagccgag cggcgtgcag ggcacgaaag ccggtttgcc aatgaagaga cggccggcat
     2941 tgaccacatg gaatccatcg acgtcctttt cgggcgcgat ggcggcgatg gcatcgtcgg
     3001 cgttgagatg cgcgggcagg ggaagctgga cgaggatgcc gtcgatagct tcgtctgcgt
     3061 tcagcgaggc gatcagatcg agaagtccgg ccttgctgat atcggcgggg gggcggtgtt
     3121 cgacagaatg gaagccaagc tcgcgcgcca gcttgagctt gctccgcaca tagacctgac
     3181 ttgcaggatt ttcgcccgcc agcaccacgg cgagtcctgg ttgacggccc gaagctgatg
     3241 cgaaagccgc ggcgcgcttg gccacttctc ctttgatctc ggccgccatg gccacaccat
     3301 tcaggatttg tgcttcagcc atcgccctac cttcgttttt tcgactgcgc gccgccctga
     3361 gagagatcgg cgagaagcct ggaaattttt tccaagagct tatcgggctc gccgtccact
     3421 gcaaccgatt tcagtctcga cttctggcct gacgtaagcg taacggcaga cttaggcacg
     3481 ccgagccatt cggcaaccag cgcgacaagc gcggcgttgg ccttgccatc ttcgggcggc
     3541 gttgtcacgt aggctttcag aaccagcttc ccaccatgat cttcaacacc gccaatgcgc
     3601 gccgcgctgc tcttgggcgt caagcgaaca tagagaacaa tgccatctgc tttccgcgtg
     3661 gcggggagtg tcatgaggga agtttaagag ttctctctcc cctggggaga gaggcagagt
     3721 gacgggcgat atccgcaatc atcggcagcg acctatctcc tgcattccgg ctcacccgcg
     3781 tcctctcgcc acaaagagga gttaaatcag ccagctcagc agaaccacgt cgcggatgaa
     3841 gatcagaccc aggatcagaa tcacgggcga aatgtccacg ccgcccagat cgggcagcac
     3901 cttgcggatc ggacggagag cgggctcggt caggcgatac aagctatcgg cgattgtgaa
     3961 gaccgcgcgg ttccggcggt tcaccacgtc gaaagcgatg agccagctca aaatcgcccc
     4021 gatgatgatc acccagatgt agagtgaaat caccatcgca ataaaaccta taagtgcatg
     4081 cattggcttc gcctccccgc aagctcgatg ccgattcttt agcttggtgc ataagtctct
     4141 tcaagggatt cgcgctgcgg cacacgcgct ttgcacctta tgcttcggca agttcgggat
     4201 gacgtcgctc tcacgggcga gcaggcgcag tgcgcgccag cccaaaccat gtggcgatta
     4261 agctaacggc ggatgccgag ccgcgcgatc aggtcgcgat agcggccttc gttcttgctc
     4321 ttaacatagt cgagcaggcg ccggcgctgg ctgacgagct tcaaaagccc gcggcgcgag
     4381 tggttgtctt ttttatgcgt cttgaaatgt tccgtcaggt tgacgattcg ttctgtgaga
     4441 acagccacct gaacttctgg ggagcccgtg tcacccggct tcgtcccata ttctccaatg
     4501 agcgcggttt tgcgctctgc ggtaatcgac atcggttatc tccgtccgct ttttgctgat
     4561 ccggcttcag gcggggatat tgaaaacccg gatcggatgc atctcgccgc gctccacctc
     4621 gccaatggcg atgaggaccc cgcgcgaggt cgcatagatt gggcctttca ggattggtgc
     4681 gttcccgcct cgtatgagga tggattgccc ccgctttagc ctggctgcat catccccgct
     4741 tacggcgagc gccgggatgc cgtccagcgc cgtctcaaca gggattatcg tgctcataag
     4801 agcatcgaag ccaccgtcac tattgcttat gtgttccagc ttttccaggg aaatcgcagc
     4861 ctcttcgtcg aatgctccca ctgaagtgcg ccggagcgcc gcgatatggc cgtagcagcc
     4921 gagcgcgcgg cccagatccc gggcgatgga gcggacgtaa gtgccctttc cgcaagcgca
     4981 gatgaggcgc gtcagatctg tcgaaatcgc ctcctcgata tcaagctcgt agaccgagac
     5041 ttgcctcgcc gccagctcaa ccgtctcgcc atcgcgagcg agatcgtagg cgcgctcccc
     5101 ttcgaccttg atcgcggaat agcagggcgg aacctgcatg atgtcgccga tgaaggctgg
     5161 cagcgccgct tcgatggcct cccgactcgg ccttgcgtcg cttgtctgcg tgggctggcc
     5221 ttccgcgtcg tcagtgtcgg tctcgatgcc ccagcggata gtgaaatcgt aggacttctc
     5281 gcaatcgacc acatgcgcga ccgtcttcgt ggcttcgccg aaagcaatcg gcaggatgcc
     5341 cgtcgcgagt ggatcgaggg tgcccgcgtg cccgaccttt tttggattga acaaccgttt
     5401 gaggcggccg agcgcctggt tcgaagtcag cccttgcggc ttatcgagga taagccagcc
     5461 attgatgtcg ttgcctttgc gccgcgccat ggtccttcgc tttacgtttc cgatttcaga
     5521 tcgcgggcga cttcgggaga gcgcagaaga tcatcaatgc gtccggcctc atcgagaatg
     5581 tgatcgcctt tgaatgcaag ctcgggcaca tatttcaact cgatgcggcg cgcaagctct
     5641 ccgcgcacaa atcgattatg gcggttaaga gccttggcaa tgatgttgct gtccttgccg
     5701 ctgagcgcgc ttgtgtagac agtcgcgtgc ttcagatcgg ggcttacccg cacctccccg
     5761 acggtgatga tctcgccctt cagttcggtg tcctcgatat cctcggtctg gaaaatctgc
     5821 gcaagcgtgt gccggagcaa ttcaccggcg cgaagctgtc tttgcgaggg gccttttgtt
     5881 gattgatgtc tcatgggtgt ttctccagaa cccgcataca taagcgaatc ccggcgagag
     5941 acaagcgcgt ttggtccatc tgattggcgg gtgtggtcgc ccacactgtc cacagcagga
     6001 agcgtaacca catattttaa attgcatttg aaaccaagtg tgctgaaatg acccaatact
     6061 ggagcagaca gcgtctattt ggacgcacat aagctgctct agaactttag aagcgatcaa
     6121 cttttgcgcc ttcaatcgat tccgattaaa ggcggggtat ctaaatctcc agctatcggg
     6181 tcttaactta tgagacgctt tttcatctca gtgatcgcgg tcaccctatc gatgctgcct
     6241 ctggcatcgg ggttatcgtc ggcggcgagt gcagctgtga atgcgcctgc gcttagcgcc
     6301 gttgatggac a
//
LOCUS       DATMGX010000858         2881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_258409, whole genome shotgun sequence.
ACCESSION   DATMGX010000858 DATMGX010000000
VERSION     DATMGX010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258409"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VNR65_14060"
     CDS             <1..602
                     /locus_tag="VNR65_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19843.1"
                     /translation="KIGVAGGPIDKSWLILRAYGMKSGVGDLAAAATPVFAAPPLLNE
                     QAAAGRIDALLNYWPFAVKLESTGYRKMMAVSQMMKSLGIENPLPLVGFVFPASLASD
                     SRGLLQSFSRAVQKGQSILATSDQEWERIRPLMKASSDEEYRMLRTRYREGLLHSWNE
                     RDREAARKLFAIVRETGGEEVTGTGVTFDPRAFWDGFVL"
     gene            586..1419
                     /locus_tag="VNR65_14065"
     CDS             586..1419
                     /locus_tag="VNR65_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ19844.1"
                     /translation="MALFSSPLSEEQERRAAKTAARREPSANARDRGRLLVSAVSLLV
                     FFAVWALAAWIAQSRHFPDPLAVGERIVAETASGQLPFHVGVTLARVTASFVLAVVIG
                     SAIGFAMGRSSRLNLFFDSWLILFLNLPALIVIILCFLWFGLNEAAAITAVALNKIPN
                     VAVIIREGTQALSPAYDELATVYRFGWWTRLREVHLPQLAPYFAAAGRSGLAIVWKIV
                     LVVELLGLSSGVGFQLNTFFQLFDVTGILAYAASFIAVIFLIETFVMRPWQLAASRWR
                     Q"
     gene            1416..2141
                     /locus_tag="VNR65_14070"
     CDS             1416..2141
                     /locus_tag="VNR65_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ19845.1"
                     /translation="MIAFAVNILRKAYESGEGPRLAIAGLSFEVQDGELVCVLGPSGA
                     GKTTALNIIAGLDSKFEGKVSFREAKPHRLSYVFQDPRLLPWRTLIENVALPLKGKAD
                     AVDIAASLLDRVGLSGGHHLYPGQASIGMQRRAAIARAFAFGPSVLLMDEPFVSLDEK
                     AARELRSLFIALWKAERVTTLFVTHNVAEAAQLATRVLVFSGSPARIAADIDLRGEEA
                     SGGNAELMLRSALEAAHASHSLS"
     gene            2230..2739
                     /locus_tag="VNR65_14075"
     CDS             2230..2739
                     /locus_tag="VNR65_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19846.1"
                     /translation="MKSALFLALASALVASTAFADDGASFVNGDWIGTGSFQMGGEIS
                     ACSEVKMRFGGSKTTYVVREASMICGNGPKQDFTAVDAFSIKDDGQITFEGGTNTKLA
                     KETKVGVVKDNKLRTINPIEADNVDDISMQKAGDFLIYNQIAGVPGKTPDYSLMAIMK
                     KDPSAAAKP"
BASE COUNT          541 a          805 c          874 g          661 t
ORIGIN      
        1 agaagattgg cgtcgctgga ggtccgatcg acaaaagctg gctaattttg cgggcttacg
       61 ggatgaaaag tggcgtgggc gatctggcgg ccgcggctac gcccgtgttt gctgcgccac
      121 cgctgttgaa cgaacaggcc gccgccgggc ggattgacgc gctcctcaac tactggcctt
      181 ttgcggtgaa gctcgagtcg accggctatc gcaaaatgat ggcggtttct caaatgatga
      241 agtcgcttgg catcgagaac ccgcttccgc tcgtaggctt tgtgtttccg gcaagccttg
      301 ccagtgacag tcgtggtctg ctgcaaagct tctcccgggc tgtccagaaa gggcaatcta
      361 tcctcgccac gtcggatcag gagtgggaac gcatcaggcc tcttatgaaa gcaagcagcg
      421 atgaggaata tcgcatgctg cggacacggt atcgcgaggg tttgctccat agctggaacg
      481 agcgcgaccg cgaggcggca cgcaaattgt ttgcgattgt gcgcgaaacg ggtggcgaag
      541 aggtaacagg gaccggcgtg acgttcgatc ccagggcttt ttgggatggc tttgttctct
      601 agtccactct ctgaagagca ggaaaggcgc gccgcgaaaa cggcggctcg ccgtgagccg
      661 agcgcgaatg ctcgcgacag gggccgcctg cttgtcagcg cggtctcgct cctggtgttt
      721 ttcgcggtct gggctttagc ggcatggatc gctcagtccc gacatttccc ggaccccctc
      781 gctgtcggcg aacgcatcgt tgccgagacc gcatcgggac agcttccctt tcatgtcggc
      841 gtgactttgg cgcgcgtcac tgcaagtttc gtgctcgccg ttgtcatcgg ctcggcaatc
      901 ggcttcgcga tggggcgctc ttcgaggctg aacctgttct tcgacagctg gctcattctg
      961 tttcttaatc tgcccgcgct catcgtcatc attctttgct ttctctggtt cgggctcaat
     1021 gaagcggcgg cgatcaccgc ggttgcgctg aacaagattc ccaatgtggc agtgatcata
     1081 cgggagggaa cgcaggcgct gtcgcccgcc tacgatgagc tggccaccgt ttaccgtttt
     1141 ggatggtgga cgaggctccg tgaggtgcat ctgccgcaac tcgctcccta tttcgccgcg
     1201 gcgggccgtt caggccttgc catcgtttgg aagattgtgc tcgtggtcga acttctgggg
     1261 ctttccagtg gcgtaggttt ccagctgaac accttttttc agctcttcga cgtcaccggc
     1321 atccttgcct atgcggccag ttttattgcc gtcatttttc tcatcgagac attcgtcatg
     1381 cgcccctggc aacttgcagc cagcagatgg cgtcaatgat cgcgtttgcc gttaacatcc
     1441 tgcggaaggc ctatgaaagc ggcgaggggc cgcggctggc catcgccggc ctctcattcg
     1501 aggttcagga cggcgaactc gtgtgcgtgc tcggaccttc gggcgcgggc aagacaaccg
     1561 cgctgaacat catcgccgga ctggattcca agttcgaggg caaggtctcg tttcgtgagg
     1621 ccaagcccca ccggctttct tacgtctttc aagacccgcg gcttctgccc tggcgaacgc
     1681 tgatagagaa tgtcgccttg cccttgaaag gcaaggccga tgcggttgac atagccgcct
     1741 ccttgctcga tcgcgtgggg ctttccgggg gccaccacct ttaccccgga caggcatcca
     1801 tcggcatgca gcggcgcgct gccatcgcga gggcctttgc tttcggacca tcagtccttc
     1861 ttatggacga gcctttcgtt tcgctggatg agaaagcggc gcgggaattg cgcagcctgt
     1921 ttatcgcgct ttggaaggcc gaacgcgtga ccacgctgtt cgtcacgcac aatgtcgccg
     1981 aggccgcaca actagccaca agggtgctcg tgtttagcgg ctctcccgcc agaatcgcgg
     2041 cggatatcga tctccgtgga gaagaggcaa gcggcggcaa tgctgagctg atgctgcggt
     2101 cagcgctcga ggcagcgcat gcgagccact ccctttcctg acatgcgggc cctcacagaa
     2161 ttttacttag ttcgcgcaca gaaccctctt acaattcctt tcaaggcgtt tttgcatcgg
     2221 agggagatca tgaaatctgc tttatttctg gccttggcga gcgctctggt ggcttcgact
     2281 gcgtttgcag acgacggagc gagcttcgtg aatggcgatt ggattggaac agggtctttt
     2341 cagatgggtg gagagatcag cgcttgttcc gaagtgaaga tgagattcgg aggctccaag
     2401 acaacctatg tggttcgtga agcctcgatg atctgcggga atgggccgaa gcaggatttt
     2461 acggcggtcg atgcattcag catcaaggac gatggccaaa tcactttcga gggagggacc
     2521 aacacgaagc tggcgaaaga gacgaaggtc ggcgtcgtga aagataataa gttgcgcacc
     2581 ataaatccga tcgaggccga taacgtggat gacatttcca tgcagaaagc cggggatttc
     2641 ctgatctaca atcagattgc tggcgttccc ggcaagacgc cggattacag tctgatggcg
     2701 atcatgaaaa aggatccgtc tgccgcagcc aaaccttgag cgccggtttc ctgcggttgg
     2761 cagtgagcgc ttctgaacaa taaagctttt cgcgttgcat cctcgcctcg gcaggacgca
     2821 attctgttgg gctgccatgt ctttgcctca acctcatgcg catgcgatgg ccccatccgc
     2881 g
//
LOCUS       DATMGX010000859         2144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_337278, whole genome shotgun sequence.
ACCESSION   DATMGX010000859 DATMGX010000000
VERSION     DATMGX010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337278"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VNR65_14080"
     CDS             <1..805
                     /locus_tag="VNR65_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWJ19847.1"
                     /translation="YLPLFIILAVLLFPFYWMALTAIKPDEQLLDLEKFNPFWTWNPT
                     FKHIYKLLFETQYPRWLWNTMLVAVSATALSIAASVLAAYAIVRLRYKGAQWVGGAIF
                     LAYLVPPSILFIPLASVVFQYGLFDTPFALILTYPTILIPFSTWLLMGYFKTIPYELE
                     ECALIDGASRWQILTKIVLPLAIPGLISSFIFCFTLCWNEFIYALTFLSSTQNKTVPV
                     AIVNEFVDGDIYRWGSLMAGALVGSLPLVILYAFFVEHYVSAMTGAVKE"
     gene            complement(1009..1257)
                     /locus_tag="VNR65_14085"
     CDS             complement(1009..1257)
                     /locus_tag="VNR65_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HWJ19848.1"
                     /translation="MFIGGGEFQNDKIMAAFRAFEAYDPASNTWDQSLPRMRIPRHGF
                     AGAVIGDQLHVVAGDIQTAGIPGVTAYTDTHDVFDFTK"
     gene            1918..>2144
                     /locus_tag="VNR65_14090"
     CDS             1918..>2144
                     /locus_tag="VNR65_14090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWJ19849.1"
                     /translation="MFQIRIHGRGGQGVVTAAAVLSVAAFLEDKHAQAFPSFGSERMG
                     APVVSFCRIDTKEIRLREPIVAPDALIVQDVT"
BASE COUNT          412 a          615 c          611 g          506 t
ORIGIN      
        1 ctatctgccg ctgttcatta ttctggcggt gctgttgttc ccgttctatt ggatggcgct
       61 gaccgccatc aaaccggacg agcaactgct cgatctggaa aaattcaatc cgttctggac
      121 ctggaacccg accttcaagc acatttataa actgctgttt gaaacgcagt acccgcgctg
      181 gctttggaac acgatgctgg tcgcggtgtc tgcgacggcg ctttcgatcg ctgccagcgt
      241 gctcgccgcc tacgccattg tccggctgcg ctataaaggc gcgcagtggg tcggcggcgc
      301 aatcttcctg gcttatctgg tgccgccctc gatcctgttc atcccgctgg cgtcggtcgt
      361 gttccagtat ggcctattcg acacaccgtt cgcactgatc ctcacctatc cgaccatcct
      421 catcccattc tcgacctggc tgctgatggg ctatttcaag accattccct acgagctcga
      481 agaatgcgcg ctgattgacg gcgcgagccg ctggcagatc ctgaccaaga ttgtgctgcc
      541 gctggcgata cccggactga tctcgtcctt tatcttctgc ttcacgctat gctggaacga
      601 attcatttac gcgctgacat tcctgtcctc gacgcagaac aagacggtgc cagtagctat
      661 tgtgaatgaa tttgtcgacg gcgatattta ccgctggggc tcgttgatgg ccggcgcttt
      721 ggtggggtcg ctgccgctgg ttatcctcta cgcattcttc gtagagcact atgtctcggc
      781 gatgacgggg gcggtaaaag aatgaaatac ctgggaactt gatctcgcgt gacacataat
      841 ctgaggttat gtgctgctca gcggcccgct cggcgctatc acgtagataa atttcgaaga
      901 cgtgcgtcaa cacagggcag cgaaacaaat ttggatgcag aaaagggtgc tcccaaccgt
      961 tctggtcgtg agcacccttg gtaatgattt agtgaggtcg gcagaccgtc acttcgtgaa
     1021 gtcaaagacg tcgtgagtgt cggtgtaggc cgtgaccccc ggaattccgg cggtctggat
     1081 atcgcccgct accacatgca gctgatcgcc gatgaccgct ccggcaaagc cgtgacgcgg
     1141 tatgcgcatg cgtggaaggc tctggtccca ggtgtttgaa gcagggtcat aggcctcgaa
     1201 cgcgcggaac gccgccatga tcttgtcgtt ttggaactcg ccgccgccga tgaacatcct
     1261 gcctttgtag atgctcccgg cgacgccgct tcgcgcgaaa ccggagccgg cggttagcgg
     1321 gctgatggct gcagcctcaa ttccctcggc tcgttcagcg ggccggaggc ggtttgcgtt
     1381 agatcaaaac cggttccccg ccatttggtt agtctgtcgt gcgcgcaccc tatgaaagcc
     1441 attttacgta gggggaggtc ttgtccaaca ttcacgacgc agaccaccgc tggcgaaata
     1501 tgtgcgagat gctcgatcga ttgggcttgg atgcatcgat gctcgcccat ggacgcctcg
     1561 ccaccgatct tcgttccgcg gttagtacat gtcagtcttg cagtgcggat caagcctgcc
     1621 aggaatggct tgtgcgcgcg ccggaatcta tcgataagcc gccggcgttt tgccccaatg
     1681 cagagctgtt cgcgtccgtg cgtgacttaa tcaatcgcgg catcgatgac gggcagatgg
     1741 cctgaaccgc caaacctcaa aaccacgcaa tatcaatgag ttgatttggc aacggagcca
     1801 aatcgttggc ggcggccaag attcgaattg cggcatatca atgcggcctc gtgcgctcgg
     1861 ctctatcttg attttacaac atcgagaaat gcagcggccg gagcgaggat tacgtcgatg
     1921 ttccaaatcc gcatccatgg acgtggcggc caaggtgtcg tcaccgcggc ggccgttctt
     1981 tccgtcgcgg cgttccttga ggataagcac gctcaggcct ttccaagttt tggttccgag
     2041 cgcatgggcg ctcccgtcgt ctcattctgt cggatcgata ccaaggaaat ccggctgcgt
     2101 gagcccatcg tcgcgccgga tgcgttgatc gtccaggacg taac
//
LOCUS       DATMGX010000860         3600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_50193, whole genome shotgun sequence.
ACCESSION   DATMGX010000860 DATMGX010000000
VERSION     DATMGX010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50193"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..1435
                     /locus_tag="VNR65_14095"
     CDS             473..1435
                     /locus_tag="VNR65_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ19850.1"
                     /translation="MNRVRDFVRFLAQAGVVPGPALPPAGPTVAPVVAEFLEWLRKHR
                     GLSERTIGFRGHLINRFLPALGPDPATYDAGRVRQVILDEARRSSTAYVRAMTSTLRG
                     YLRFLAARGACSPWLDQAVPTVPHWRLSALPRYLPVTDVERLIASCDLATPSGIRNKA
                     ILLLLARLGLRAGDILAMRLGDVDWKEGTIRVSGKGRREVCLPLPQDAGDALLYYITR
                     VRQSVVHDQIFLRSLAPFRPVRQISSVVGLGLKRAGITNAPSRGARLLRHSAATGMLR
                     AGATLDAIGAVLRHRSPDTTMQYAKVDTGMLGAIAQPWPGDVTC"
     gene            1429..2340
                     /locus_tag="VNR65_14100"
     CDS             1429..2340
                     /locus_tag="VNR65_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ19851.1"
                     /translation="MLSVDMARYVELHRSVGFKFRTQHGLLKSFTAFAEAHGDEFVRI
                     DRALDWAAQASSPPQRRNRLLEVRRFALAMRPEDARYEAPAADALGRAFYRRRIAHIY
                     SPAEIAHLIQEAGRLGPVGSVRPKMYATLFGLLATTGLRISEALALKLKDVTEDGLLI
                     RETKFCKSRLVPLHATTQQALNAYIGARSRLGTVDDSLFISICGRTPAYTTVDAVFLK
                     LARSIGLRGDPGQRGARIHDLRHSFAVRSLEQCQNGGDSVARHLVALSTYLGHAHATD
                     TYWYLQATPLLMEQIANVSEALHQRGE"
     gene            2342..3340
                     /locus_tag="VNR65_14105"
     CDS             2342..3340
                     /locus_tag="VNR65_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ19852.1"
                     /translation="MTALAPYLSAFLLDHLPRERNASPHTCDAYAYSFKLLVCFAAGR
                     LHVKPCQVELEQLDVPLVLAFLDHIESDRGNSARSRNARLAAIHSFFRFLESRLVSCL
                     NQARQIHAIPMKKADEALVGYLTPDELRALLDAPNRSTLSGIRDRAMLHLAFAAGLRV
                     SELVGLRMEGLDQATFATVHVMGKGRRERVLPLWKETVTAVKAWLAVRPCHGDQELFL
                     NAPGHAMTRSGFEYILAKHVSTAARTEPSIATKRVTPHVLRHTCAIHTLRATGDIRKV
                     SLWLGHATLQSTEIYLRADLTAKLEALAASVPPMVKAGRFRAPDQLLAMLRRAART"
BASE COUNT          680 a         1107 c         1066 g          747 t
ORIGIN      
        1 gcgccgccac ccgcttatgc agagctctgc ataagcgggt ttatgcggcg cgaatggtta
       61 tgcagagtct cgtggcggtt tgaaacggga actgtcgatt ttgctgggcc gtgccatcat
      121 gtgcgggatc tcgtgctcaa cataactttg ggcttctcct ggtggggcaa cgcacgccaa
      181 ccgaaggaga accaccgtgc caaacaccgc agattcgcca tcacgtcggc cgcatatgga
      241 tgcgggaaac atcaggcctt gggtcgagca gttcgtgagc cacctcgttg gcctgggcca
      301 cacgcctttc acagtgaatg gttattccga accggctcgt cactttgccc attggatctg
      361 ctagactggg gttacgctgt gtgatgttga tgacaggacg gtcgagcaat tcgcacagca
      421 caaatgtcaa tgcccaggcg gacgccgcct tcattgcgtc tcaaagagat acatgaaccg
      481 cgtacgtgac ttcgttcgct tccttgcaca ggccggcgta gtaccggggc ctgcactgcc
      541 tcccgctggg ccgacagtgg ccccggtcgt cgccgagttt ctggaatggc tgaggaaaca
      601 ccgaggcctt tccgagcgaa cgatcggctt tcgaggacac ctgattaaca gatttctgcc
      661 agcactcggc cccgacccgg caacctatga cgccggtcgt gttcgtcagg tcatcctcga
      721 cgaagcgcgg cgaagttcca ccgcctacgt aagagcaatg acgagcacgc tgcggggcta
      781 cctgcggttc ctggcggctc gcggcgcctg tagcccgtgg cttgaccaag ccgttccaac
      841 tgttccgcat tggcggttat cggctctgcc acggtatctg ccggttaccg atgtcgagcg
      901 gttgatcgcc tcctgtgatc ttgcgactcc gagcggtatt cggaataagg ctattctcct
      961 tctcctggca cgtcttggtc tgcgggccgg tgacattctg gccatgcgtc tcggcgacgt
     1021 cgactggaaa gagggcacaa tccgggtttc tggcaagggc cgacgggagg tctgcctgcc
     1081 gctaccgcaa gatgccggcg acgccctgct ttattacata accagggttc gtcagagcgt
     1141 tgtccacgac caaatctttc tacgatcact tgctcccttt cgacccgttc ggcagatatc
     1201 cagcgtcgtt ggactgggac tgaagcgggc cggaattacc aatgccccct cacgcggagc
     1261 ccgtctgctc cggcactcgg cggccaccgg catgctgcgc gctggtgcga ccctcgacgc
     1321 cattggagcc gtccttcgac accggtcgcc cgacaccacc atgcagtacg ccaaagtgga
     1381 cactggaatg cttggcgcga ttgcgcagcc gtggccggga gatgtcacat gctgagcgtc
     1441 gacatggcac gatacgtcga gcttcatcgt tcggttggat tcaagttcag aacgcaacac
     1501 gggctattga aaagctttac agccttcgcg gaagcccatg gagacgaatt tgttcgcatc
     1561 gaccgcgcgc tcgactgggc ggcacaagcg tcgtcgccac ctcagcggcg caacaggtta
     1621 ctcgaagtcc gacggttcgc actggccatg cgtccggagg atgctcgcta tgaagcgccg
     1681 gcggcagatg ccctgggtcg agcattctac cgacgacgga ttgcccacat ctactcgcca
     1741 gccgaaatcg cccacctcat tcaggaagct ggtcgactgg gacctgttgg ttctgtcagg
     1801 ccaaagatgt acgccacgct cttcggcctt cttgccacga ccggccttcg catttcggag
     1861 gcattggcgc taaaactcaa ggacgtgacg gaggacgggt tgctcatacg cgaaacgaag
     1921 ttctgcaaga gccgcctcgt gccattgcat gcgaccacgc aacaggcctt gaatgcctat
     1981 atcggcgctc gcagtcggct tggtacagtt gacgattctc tgttcatctc gatctgtggg
     2041 agaactccgg cttacactac ggtggacgcc gttttcctga agctggcccg atcgatcgga
     2101 ctgaggggcg accccggcca gcgcggtgcg cgcattcatg atcttcgtca ttcgttcgcc
     2161 gtgcgctccc tcgagcagtg ccagaacggc ggagattcgg tcgctcggca ccttgttgcc
     2221 ctcagcacct acctcggcca cgcgcacgcg acagacactt actggtacct gcaagctacg
     2281 ccgttgctga tggagcagat cgccaatgtc agcgaagcgc tgcatcagag gggagagtag
     2341 catgaccgcg cttgcaccct acttgagcgc atttttgctc gatcacctgc cacgcgaacg
     2401 caatgcaagt ccgcatacct gcgatgcata cgcctacagc ttcaagcttc tcgtctgctt
     2461 cgcagcaggc cggttgcacg tcaagccctg ccaggtggaa ctcgaacagc tcgatgtgcc
     2521 gctggtcctc gcttttcttg atcacatcga gagcgaccgg ggtaattcag cacggagccg
     2581 caacgccaga ctcgctgcta tccactcgtt cttccgcttc ctggagtccc ggttggtgtc
     2641 ctgtctgaac caagcgcgtc agatccatgc aattccgatg aagaaggctg atgaagccct
     2701 ggtcggatat ttgaccccgg acgagttgcg ggcgcttctc gatgcaccga accggagcac
     2761 gctgtcgggt atacgcgacc gcgcgatgct gcacctggcc ttcgcagcgg gcctacgtgt
     2821 ttccgagttg gttggcctcc gcatggaggg gcttgaccag gcaacgttcg ccaccgttca
     2881 cgtcatggga aaggggcggc gcgagcgcgt gctgccactg tggaaagaga cagtcacggc
     2941 agtaaaggcg tggctcgccg tgcgcccatg ccatggtgac caggaattgt tcctcaatgc
     3001 ccccggtcac gccatgacgc gatccggatt cgagtacatc ctcgcgaagc atgtatccac
     3061 ggccgcgcgc acggagccat ccatcgccac aaagcgcgtg accccacacg ttctgcgcca
     3121 tacctgcgcc atccacacgc tccgagcgac cggagacatt cggaaggttt ccctctggct
     3181 tggtcatgca acccttcaaa gcacagaaat ctacctgcgt gccgatttga ccgcgaagtt
     3241 ggaagcgctt gcggcgagcg tcccgcctat ggtcaaggca ggtcgcttcc gcgcaccaga
     3301 tcagctactg gcgatgctgc ggcgggcggc ccgaacctga aattatgccg agtgaaattt
     3361 gcctacaatg gcggcgggct ttcatgctcg ccgtcgactc tgcataacaa ttcgcgccgc
     3421 ataaacccgc ttatgcagag ctctgcataa gcgggtggtg gtgatggatg tggccacgtg
     3481 acgttcaggc ctgacgggga gatccgcggg gccggtagtc accgggtatg ggaggtgcgc
     3541 ggacgagggc ggcgttttcg gttctgttgc aaagtttgtt agaagtcgcg cttggccccc
//
LOCUS       DATMGX010000861         5575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_386796, whole genome shotgun sequence.
ACCESSION   DATMGX010000861 DATMGX010000000
VERSION     DATMGX010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386796"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VNR65_14110"
     CDS             complement(<1..937)
                     /locus_tag="VNR65_14110"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ19853.1"
                     /translation="MSATPSLNATTDDGRLELLASGAWTAVHAGELEAAIGRAADEAA
                     KAGDVSIDMRNVAAFDTYGAWLLERLIRRRSGAGQQTGVVGLREEFQGLLQDVHTVNR
                     KKEAPEAKRHGLIPALGEFERAVTEQARGFLPFLDMLGAIGVAAVRVLRRPRDFRFTS
                     LVHQLDRVCWQATPIIVLITFLIGCIIAQQGFFHFRRFGAENYVVDMVGILILREIGV
                     LLVTIMVAGRSGSSYTAELGSMKMREEIDALRTMGLDPVEVLILPRVLALVIALPILT
                     FLGSMAALYGGGLVAWLYDGMSPQIFIARLHEAITV"
     gene            complement(2090..2743)
                     /locus_tag="VNR65_14115"
     CDS             complement(2090..2743)
                     /locus_tag="VNR65_14115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ19854.1"
                     /translation="MTMTASPAEKMDVIYRRQRFIYDATRRYYLFGRDRLIEDLEPPA
                     NGTVLEIGCGTARNLIRAARLYPEARLYGLDVSEAMLRSARASVERCSLAARILLRKG
                     DATAFDPEQLFALHGFDRVFISYALSMIPAWRDVVRHAAACVAPGGKLLIVDFGDFSG
                     YPSLVQSAQSAWLRRFSVVPIPAMDRKIGMLAEQMGFSSGSEKLYGGYAMQFCLQHR"
     gene            complement(2749..3951)
                     /locus_tag="VNR65_14120"
     CDS             complement(2749..3951)
                     /locus_tag="VNR65_14120"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HWJ19855.1"
                     /translation="MIGAAVQRSKPLSAEGLRERLFTLAFSNLVYAQIWEDPAVDLEA
                     LALDRNCRVVTIASGGCNVLSYLPAGPAHIAAVDINAAHVALNRLKLAAARYLPNYES
                     FYRFFGAADHAANVTAFKFHILPHLDPLTANYWHGRHGLRRRRRIRRFTNNFYRAGLL
                     GTFIGASHLVARLNGVNPRMILEAETLEEQRRIFDREFAPLFNRRMLRWLVNRPATLY
                     GLGIPPAQYKALAGDDEGGMAAVLRKRLERLACGFPLSDNYFAWQAFGRRYAPAGGPL
                     PPYLNPMNFALVRENASRVSVHHASLTAYLAGSPSGSLDRYVLLDAQDWMGDATLAQL
                     WNEITRTARPGARVIFRTAAEQTILPGRIPETILDRWHYAAEVSHELTQKDRSAIYGG
                     FHLYTFKG"
     gene            complement(4010..4882)
                     /locus_tag="VNR65_14125"
     CDS             complement(4010..4882)
                     /locus_tag="VNR65_14125"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HWJ19856.1"
                     /translation="MQRQFRTLFVSDLHLGARACQAEAFLDFLRHYDADTIYLVGDII
                     DFWSVKRSPLWLQSHNDVLQKLLRKARKGTRIIFIPGNHDEAVRGYCGMSFGAIEIHH
                     DIVHETADGRRFLVMHGDEFDVIVRYAKWLAFLGDRSYEFALWCNQPLNLVRRRLGFG
                     YWSLSGYLKLRVKTAVNFIGEFEQAISQEAKRRSVDGVICGHIHHMADLMIDGIRYLN
                     CGDWVESCTAIAEDFDGRLSQIRWLPGSMETSPALAAPVPSFALEGIPSRAASGRMAT
                     TSPVRARQYTASIE"
     gene            complement(5036..>5575)
                     /locus_tag="VNR65_14130"
     CDS             complement(5036..>5575)
                     /locus_tag="VNR65_14130"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEMT/PEM2 methyltransferase family protein"
                     /protein_id="HWJ19857.1"
                     /translation="ELKVWPTPGKGSWQSRVFWSLFRTLNVATLALAFLDWQRWEGFS
                     LGRGVGASLAIVCFALYGWACYALGRRNLYCGRDGLVTGGIYSWTRNPQYATAIPAYL
                     GLAVASDSIAVFTIASLLTLSFVLMAFAEEHWLEAAYGADYPCYRLEVARFYNWRHAL
                     AVLKQEVAPAKEAPKEARF"
BASE COUNT         1269 a         1587 c         1642 g         1077 t
ORIGIN      
        1 tcaccgtaat cgcttcgtgc agccgcgcga tgaagatctg cgggctcatc ccgtcgtaga
       61 gccatgccac gagcccgcct ccataaagcg cggccatgga accgagaaac gtcagaatcg
      121 gcagagcgat gacgagcgcg agcacccgcg gcaggatcag cacctccacc ggatcgaggc
      181 ccatggtgcg aagcgcgtcg atctcctcgc gcattttcat ggagccgagt tcggccgtgt
      241 aagaactgcc ggaacgcccc gccaccataa tggtcacgag cagaacgccg atctcgcgca
      301 gaatcagaat gccgaccatg tcgacgacat agttctcggc cccgaaccgg cggaagtgga
      361 agaaaccctg ctgggcgatg atgcagccga tcaggaaggt aatcagcacg atgatgggcg
      421 tggcttgcca gcagacgcga tcgagctgat gcaccagcga ggtgaagcgg aagtccctag
      481 ggcggcggag gacgcggacg gcggcaacgc cgatggctcc gagcatgtct aggaaaggaa
      541 gaaagccccg cgcctgctca gtgacagcgc gctcaaactc gccgagggcc gggatcagcc
      601 cgtgcctttt ggcctccggg gcttccttct ttcggttgac ggtatgaacg tcctgcagaa
      661 gcccctggaa ctcctcgcgc agaccaacca cgccggtttg ctgcccggcg cctgaccttc
      721 tccggatcag ccgctccaga agccacgccc cgtatgtatc aaacgcggcg acattgcgca
      781 tatcgatgga cacgtcgcca gccttcgcgg cctcatccgc cgccctgccg atcgccgctt
      841 caagttcgcc tgcgtggaca gcagtccatg cgcccgatgc cagcagctcg aggcggccgt
      901 catccgtagt ggcattcaag gacggtgttg cgctcatggc tgcacacccc aaaagcttca
      961 cggggaagcc ttctatagcc aagatttagg atgcttcaga agtttccaca atcgctccga
     1021 taagcgctgg agtcaaaacc gctctgcgaa ggggttatac caaccgtccg gaggttgcgg
     1081 ctcgctcatg aaggtatacc gtcggttaaa attggctttg cttcgtaaaa tagccataag
     1141 taaatatcta agcaacttaa cagccatggg gaaaggcatc gtgacttatg gcggttcgta
     1201 tgagatctcg ccagcaactt ctcctgggcg gcggaccagg caaagagtgg ggatgcctga
     1261 tccgcccgac agcgccaact ggatacaacc cactcgctat acaaataggc gtgtcagaga
     1321 gcgattagcg ggcttatgtg aagctgcgat gaaaaacgca ccttgagcgc agcaattcac
     1381 cgtttattgc ggcgtaaggt tctttcattc cggagacccg ggcggtcccc tgattgccat
     1441 tgggcgggag cagttgataa cgctcgccct tgagcagcgc cgcaagaatt caaaggcgct
     1501 ggcggcagcg ggttacattc ttggacgcca cgaggcgaat gtgccctcgt ctggagcaag
     1561 gccaagcgcg aatgggagca attggaaaag ccttcgagct tttggcgata gggctttgtt
     1621 catcacactg cagtcccgcg cgcggcctgg ttgccaaggt gactactgtc aagccaaaaa
     1681 tgtcccctgg attggggata tgcgggtccc ccgaattggg gataactcat aattggcctg
     1741 taaacgcgcg cagaatttga tttatgtcaa gcctgaactt cgataaggta tgaggtgcaa
     1801 tagaggctcg cgggcattag acctaggttg caaacggcag gttcgaatga tccggcttcg
     1861 ctgccattat ataaccggac aaaagaaagc tacttgtgag tttttgagta ttggttcgga
     1921 gttgaagtca tgcctgtcca cgaagccatc aactttacct tgacgcttgg gaccgcatta
     1981 acattgttcc tgggcatgca atacataggg catgctttgg tacgatactt caacagttaa
     2041 gttttcggat cttaccaggc tttcctgata actgtctgca gttttgcgat cagcgatgct
     2101 ggagacagaa ctgcatggca tagccgccat agagtttttc gctgcctgac gagaacccca
     2161 tttgctccgc aagcataccg atctttcgat ccattgcggg aatgggcaca actgagaagc
     2221 ggcgcagcca cgcagactgc gcgctctgca caagcgacgg atagccgctg aaatcaccga
     2281 agtcgacgat aagaagctta cctcccggcg caacgcaagc ggctgcgtgc cggacaacgt
     2341 ctcgccaagc cgggatcatt gaaagggcgt atgaaatgaa gacacggtca aagccgtgga
     2401 gagcaaagag ttgctccgga tcgaaagcgg tggcgtctcc tttgcggaga agaatgcgcg
     2461 ctgcgagact gcaccgctca acagaggcgc gcgccgaccg cagcatcgct tccgagacat
     2521 ccagtccgta aagccgggcc tccgggtaga gccgggcggc tcggatgaga ttgcgcgccg
     2581 tgccgcaacc aatttcaaga acagtgccgt tagccggggg ttcaagatcc tcgattaggc
     2641 gatcgcggcc aaaaagatag tagcgccgcg ttgcatcata gatgaagcgc tggcggcggt
     2701 agatcacatc catcttttcc gccggcgaag cggtcatggt catctgcctc atcccttgaa
     2761 cgtgtaaaga tggaagccgc cataaatggc ggaacgatcc ttctgggtca actcgtgaga
     2821 gacctctgct gcatagtgcc aacgatcgag aatagtctcc ggaatacgtc ccggaaggat
     2881 cgtctgctct gcggcggtgc ggaaaatcac gcgtgcgccc ggtcttgccg tccgggtgat
     2941 ctcgttccaa agctgcgcaa gcgtcgcatc gcccatccag tcctgtgcat caagcagaac
     3001 gtagcgatca aggctgccgc tcggagaacc cgcgaggtag gcggtcagcg aagcatgatg
     3061 aaccgagacc cgcgacgcat tctcccgcac cagggcaaaa ttcataggat tgagatacgg
     3121 tggcagcgga ccgccggcgg gggcgtatcg gcggccaaac gcctgccagg cgaaatagtt
     3181 gtccgaaagc gggaagccgc aggccagccg ttctaggcgt ttccggagca ccgcggccat
     3241 gccgccctcg tcgtcgccag caagcgcttt atattgcgcg ggcggaatgc caagcccgta
     3301 gagcgtggcc ggacggttga cgagccagcg caacatgcgc ctgttgaaga gaggcgcgaa
     3361 ttcacggtca aagatccggc gctgctcctc cagcgtctca gcttccagga tcatccgcgg
     3421 attgacgccg ttcagcctcg cgacgagatg gctggccccg atgaatgtgc cgagaagacc
     3481 ggcacggtag aaattgttcg taaagcgccg gatgcggcgt ctgcggcgga gcccgtgccg
     3541 tccgtgccaa tagtttgccg tcaacggatc gagatgcggc aggatatgaa atttgaaggc
     3601 ggtgacattt gcggcatggt ccgctgcacc gaaaaaccga tagaaggact catagttggg
     3661 caagtaacgg gcggcggcca gcttgagccg atttaaggcg acatgcgctg cgttgatgtc
     3721 taccgccgcg atgtgtgcgg ggcctgccgg gagatagctg agcacattgc agccgccgga
     3781 ggctatggtg acaacacggc agttgcgatc aagcgccaga gcctccagat cgaccgccgg
     3841 gtcttcccag atttgagcat agacaaggtt cgaaaatgcc agcgtgaaaa gacgctcccg
     3901 taagccttca gccgacagcg gcttgctccg ttggaccgct gcgccgatca tggtggcgct
     3961 cctgcgagtt ttggcttctg cgaggtcgcc caaatcaacc ctcctgactt tattcaattg
     4021 aggcagtgta ttggcgtgcc ctgacggggc tggtggtggc catgcgtccg gaagcagcgc
     4081 ggcttggaat accctcaagg gcaaagcttg gaacgggagc ggccagcgcc ggcgacgtct
     4141 ccatcgaacc cggaagccag cggatctggc tgagccttcc gtcaaagtct tcagcgatag
     4201 ctgtgcagct ttcgacccag tcgccgcaat tcagatatcg aattccgtcg atcatcaggt
     4261 cggccatatg gtggatatgg ccgcagatca cgccatcaac gctccggcgc ttggcttcct
     4321 gcgaaatggc ctgctcaaat tcgccaatga agttcaccgc tgttttgaca cgcagcttca
     4381 gatagcctga gagggaccag taaccgaagc caagcctgcg ccggaccaag tttagcggtt
     4441 gattgcacca tagcgcaaat tcataggagc gatctcctag gaacgccagc cacttggcgt
     4501 aacgaacgat cacgtcgaac tcatcgccat gcatgacgag aaagcggcgg ccatccgctg
     4561 tctcatgcac gatgtcatgg tggatctcga tggcgccgaa actcattccg cagtagcctc
     4621 gaacggcctc gtcgtgatta cccggaatga aaatgatcct cgttcccttg cgggctttgc
     4681 gaagaagctt ctgcagcaca tcattatgcg attgcagcca aagtgggctc cgcttgacgc
     4741 tccagaaatc gatgatatca ccgacgagat agattgtgtc ggcgtcgtag tggcgcagga
     4801 aatcaagaaa ggcttccgcc tgacatgccc ttgcgcccag atgcaaatcc gagacgaaaa
     4861 gggtccggaa ctggcgttgc ataactgctc gatgttgatc gccgtgctga aagcttgcgg
     4921 aagtcatttc ttcaggtcct ctatctcgcc cgccaataac ccgcttttct ctgagcctgg
     4981 cgactatggt gagcagcgaa ggccgttcgg gattagctgg atcgcataac ggcattcaga
     5041 aacgcgcttc cttgggggct tccttcgcgg gcgctacctc ctgcttgaga accgccaacg
     5101 catgtcgcca attgtaaaag cgtgcaactt caagacgata gcacgggtaa tcggcgccat
     5161 aagcggcttc cagccagtgt tcttcggcga acgccatcag gacaaagctc agtgtaagca
     5221 gcgatgcgat cgtaaaaaca gcgatcgagt cggaggcgac cgccaggccg agataagcgg
     5281 ggattgcggt ggcatattgc gggtttcgcg tccagctata gatgccgcct gtgacgagac
     5341 catcgcgccc gcagtaaagg ttcctgcggc caagcgcata acaagcccaa ccatagagag
     5401 caaagcagac aatggcgagg ctggcgccca caccgcgtcc cagagagaag ccttcccagc
     5461 gctgccaatc aagaaatgcc aaagcgaggg tagcgacatt cagagttcga aaaaggctcc
     5521 agaaaacgcg gctctgccag cttccctttc cgggggttgg ccagaccttc agttc
//
LOCUS       DATMGX010000862         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_235833, whole genome shotgun sequence.
ACCESSION   DATMGX010000862 DATMGX010000000
VERSION     DATMGX010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235833"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VNR65_14135"
     CDS             <1..1023
                     /locus_tag="VNR65_14135"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HWJ19858.1"
                     /translation="PVYGQPAYGGPREPPVFGSSNPRCRELEAQLTNGSPNAGQDQLP
                     RIENDMRQADAEFHRAQSDADRSNCYEDMFLFGRSLRRTPHCMDLDRQVQAAKASLGQ
                     LRAQRDAIARTNSPRGRRDDIVAELARNHCGEQYVHERETQRSHSSSIFSFFSNEEQS
                     APDEPPPSSLAQPPAQGYGGGSSSYRTLCVRECDGFYFPVSNYASEAHFRDDEAKCHS
                     LCASPANLFYHRADQDVDQMVSLNGRPYASMPYAFRNRKVYIRGCSCNAREYSPEQIA
                     KSEEALKISKRADASAGKSDAAFARRISQAVQNAPAAAQPVAATPAPGDGATPLEPVP
                     QPNPTR"
     gene            complement(1007..1720)
                     /locus_tag="VNR65_14140"
     CDS             complement(1007..1720)
                     /locus_tag="VNR65_14140"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HWJ19859.1"
                     /translation="MTKREPNLDRSFKVKLKKTRGRTVSSKHWLERQLNDPYVAAAKR
                     EGYRSRAAYKLEEIAAKYGLLKPGDVVVDLGAAPGGWSQIAAKCVHAAEDKGAVVALD
                     FLPFDAIPGVTILLQDFSAPEADEKLLSALAGRRPNAVLSDMAAPTTGHRQTDHLRIM
                     GLAEEALAFAVQVLAPGGTFLAKVFQGGSEKALLVLLKRDFANVRHIKPKASRAESPE
                     LYVLATGFRGSAASPIESD"
     gene            complement(1683..>2548)
                     /locus_tag="VNR65_14145"
     CDS             complement(1683..>2548)
                     /locus_tag="VNR65_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19860.1"
                     /translation="GVTRSRLVATEACRIAKNGPDFIARVKRETGLALEIIGRETEAR
                     LAVSGCASLIDKQSDYALIFDIGGGSSEFVWLNLRNGRRSKRISIEDRLKIQDCIDAW
                     TSLPFGVVTLAERFGSNRAEGFQFEDMVNFVQAKLEPFSQTNGISEKVAHAGTHLLGT
                     SGTVTTIAGVYLGLSEYQRAKVDGCWLSTDSIRDVTKRIAAMNWEQRAAQPCIGAERA
                     DLILAGCAIMEAMLRVWPVKSLRVADRGLREGILATLIAEDAIRERASPAIHSPTFIH
                     DQARAKSGPVF"
BASE COUNT          497 a          795 c          750 g          506 t
ORIGIN      
        1 cccgtctatg gccagcctgc ctatggcggc ccgcgtgagc ctccggtgtt cggttcctcg
       61 aacccccgtt gccgggagct tgaggcgcag ctgacgaacg gctcgccaaa tgccgggcag
      121 gatcagcttc cccgcattga aaatgacatg cgccaggccg acgcagagtt ccatcgcgct
      181 caatcggatg ctgatcggtc taactgttat gaagatatgt ttctgttcgg ccggtcgctt
      241 cgccggacac cccattgcat ggatctcgac cggcaggtcc aggccgcgaa ggcctcgctc
      301 ggccaactga gggcgcagcg cgacgcaatt gcgcgaacca actcaccgcg tggaaggcgc
      361 gacgacattg tcgcggagct tgcgcgcaac cattgcggcg agcagtatgt gcacgaacgc
      421 gaaacacagc gcagccacag ctcctcgatt ttctctttct tcagcaatga ggaacaaagc
      481 gcgccggacg agccgccacc gtcgagcttg gcgcagcccc ctgcacaggg atatggcgga
      541 ggttcatcct cttaccggac attgtgcgtt cgcgaatgcg acggcttcta ttttcccgtc
      601 agtaactacg catcggaagc tcatttccgc gacgacgaag cgaagtgcca ctctttgtgc
      661 gcttctccgg ccaatctctt ctatcaccgg gccgatcagg atgtggacca gatggtctcc
      721 ctgaatgggc ggccctatgc atcgatgccc tatgcgttcc ggaaccggaa agtctatatc
      781 aggggctgtt cgtgtaacgc gcgcgaatat tcgcctgagc agattgcgaa gtcggaagaa
      841 gcgctgaaaa tctcgaagcg ggccgatgca agcgcaggca aatcggatgc cgctttcgca
      901 cggcgcatct cgcaagcggt gcagaacgcg cctgcagcag cccaaccggt tgcagccact
      961 ccggcaccgg gcgatggcgc aacgcccctt gagccagttc cccagcctaa tccgactcga
     1021 tagggctggc tgcgctccca cgaaatcctg ttgcgaggac gtaaagctcc ggtgactccg
     1081 cccggcttgc tttcggcttg atgtgccgga cgttcgcgaa atcgcgcttc aaaagaacaa
     1141 gcaaggcctt ctcgctccct ccctggaaaa ccttcgcaag gaaagttccg cccggcgcca
     1201 gaacctggac ggcgaacgcc agcgcttctt ctgcgaggcc catgatgcgg agatggtcgg
     1261 tttgccggtg cccggtagtc ggagcggcca tgtcggatag aaccgcattc ggcctgcggc
     1321 ctgccagcgc cgacagcaat ttctcgtccg cctctggcgc gctgaagtcc tgcagcagga
     1381 tggtcacgcc cgggatcgca tcgaatggca gaaaatcgag cgccacgacg gcacctttgt
     1441 cttcagccgc gtgaacacat ttcgccgcga tctggctcca accgcccgga gcagctccaa
     1501 gatcgacgac aacgtcgccg ggcttcaaca gcccatactt ggccgcgatt tcctcaagct
     1561 tgtaggcagc ccggctgcga tagccttcgc gcttcgccgc agcgacgtaa gggtcgttca
     1621 gctgccgctc aagccaatgc ttggacgaga ccgtgcggcc acgagtcttc ttcagcttga
     1681 ccttaaaaga ccggtccaga tttggctctc gcttggtcat gaatgaatgt ggggctgtgg
     1741 attgccggcg acgcacgctc ccggatggcg tcttcggcga tcaatgttgc aaggatgcct
     1801 tcccgcaggc cgcgatcggc aacgcgaagg gacttcaccg gccacactct cagcatggct
     1861 tccatgatgg cgcaaccggc gagtataagg tcggctcttt cagcgccaat gcaaggctgg
     1921 gccgcgcgtt gttcccagtt catggcggcg atgcgttttg tgacatcccg gatgctgtcg
     1981 gtggatagcc agcatccatc caccttggct cgttggtatt cggagaggcc gagataaacg
     2041 ccagcgattg tcgttaccgt tccagaggtg cccaggaggt gcgtgccagc gtgagcaacc
     2101 ttttcgctaa tgccgttggt ttggctgaaa ggctcaagct tcgcttggac gaagttcacc
     2161 atgtcctcga actgaaagcc ctccgcgcgg ttgctgccaa agcgttccgc gagcgtgaca
     2221 acgccgaatg gcaacgacgt ccaggcgtcg atgcaatcct gaattttcag gcgatcttcg
     2281 atcgaaatgc gctttgagcg gcggccgttc ctgagattga gccagacgaa ttcggatgaa
     2341 ccaccgccaa tgtcgaagat cagcgcatag tcgctttgct tgtcgataag ggatgcgcac
     2401 cccgagacgg cgagccgagc ctccgtttcg cgcccgatga tttcaagcgc aagccccgtc
     2461 tcgcgtttaa cgcgggcgat gaaatcaggc ccgttttttg caatgcggca ggcttccgtc
     2521 gccaccagcc gggagcgggt cacccctc
//
LOCUS       DATMGX010000863         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_114629, whole genome shotgun sequence.
ACCESSION   DATMGX010000863 DATMGX010000000
VERSION     DATMGX010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114629"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VNR65_14150"
     CDS             <1..488
                     /locus_tag="VNR65_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWJ19861.1"
                     /translation="AIEEQEIAGCRLELFVINFEKDKMTLRVPTSKSQSAGMRKLSDD
                     AVMTRALDTLRGRARVKRTMWSRRAQEYEAKINSGDLITIAEVVRDLYRSDSQPEQSY
                     SERQLYEAALDRMAREIAAAQDVDESIAVQKVAEALTKAGRLHRKHDGKPEEHEKKEA
                     A"
     gene            complement(514..1224)
                     /locus_tag="VNR65_14155"
     CDS             complement(514..1224)
                     /locus_tag="VNR65_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HWJ19862.1"
                     /translation="MAITGPQTLHLADGRMVHLAEVFTPASAEKAGLDPSSNATAYLR
                     STALGRKVEVKFGGSHHDRYGVTIAHIFVAGERPLWLQEGLVNAGLAQAFPQPDNHTC
                     SKQLASIEEKARDENRGYWGLALFRVLPARETRSILNVVQTYQIVEGDVTFVTHAAGR
                     TTLHFAENSKASFTATLEPAAQRRLSEPNPDDWQGEKVRIRGWIERKKGPTITIIQPE
                     QIEVIQQEPPKSAPDSPR"
     gene            complement(1592..1946)
                     /locus_tag="VNR65_14160"
                     /pseudo
     CDS             complement(1592..1946)
                     /locus_tag="VNR65_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046622600.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2034..2110
                     /locus_tag="VNR65_14165"
     tRNA            2034..2110
                     /locus_tag="VNR65_14165"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2068..2070,aa:Arg,seq:cct)
BASE COUNT          469 a          571 c          649 g          481 t
ORIGIN      
        1 ttgcgatcga agaacaggaa attgctggtt gcaggcttga actttttgtc atcaatttcg
       61 aaaaagacaa gatgaccctg cgcgtgccta cttcgaaaag tcaaagcgcg ggcatgcgga
      121 aactttcgga cgacgcggtt atgactcgcg ccctggacac cctgcgcggc cgcgcgcggg
      181 tgaagcgcac catgtggagc cgccgcgctc aggagtatga ggcgaagatc aattcgggcg
      241 accttatcac gatcgcggag gtggtccggg atctctatcg ttctgattct caaccggaac
      301 agtcgtattc tgagcgccag ctttatgaag ctgcattgga tcgtatggcg cgggaaattg
      361 ccgcggcgca ggatgtggac gagtccattg ccgttcagaa ggtcgccgag gcattgacca
      421 aggctgggcg tctccaccgc aagcatgatg ggaagccgga agaacacgaa aagaaagaag
      481 cagcgtaatc taatcaatcc aggcattgtt cttttagcgc gggctgtctg gtgccgattt
      541 gggcggctct tgctgaatca cttcgatctg ttctggttga ataatcgtaa tggtcggccc
      601 tttcttgcgc tcgatccatc cgcgaatgcg gactttttct ccttgccagt catcagggtt
      661 aggctcagag agtcttctct gggcggctgg ctcgagagtt gcggtgaagc tggctttgct
      721 gttttcagcg aaatgaaggg tagtgcggcc tgctgcatgt gtgacaaaag tcacgtcgcc
      781 ctcaacgatt tgatatgttt ggacaacgtt taggatggag cgtgtttcac gcgcgggcag
      841 aaccctgaac agcgctagtc cccaataccc acggttttcg tccctggcct tctcttcaat
      901 agaggccagt tgctttgagc aggtatgatt atcgggttgc ggaaacgctt gggcgagccc
      961 agcgttaacg agaccctcct gtaaccagag ggggcgttcg ccagccacga aaatatgagc
     1021 gatggttacg ccataacgat catgatggct gccgccaaat ttgacctcta ccttccgtcc
     1081 caacgccgtt gaccgtagat atgctgtggc gtttgaagat gggtctaagc ccgctttctc
     1141 ggcactcgca ggggtaaaaa cctctgcaag atggaccatg cggccatcgg cgagatggag
     1201 ggtctggggg ccggtgatcg ccaaaacaat gctctcgccc ccgggttcaa ggtgacagtt
     1261 ctcttcctcc gccctctgac ggtgctggcc gtaagccgat gaggcaacga agagcagaag
     1321 gcccagcaca agcgaagtgc gacataaaca ctcgagcaga agcaaagtaa ggcggaccaa
     1381 catagcctct tctttaggga ttgagtccat cgcactcaag tcaaaaggcc ggcgtccaaa
     1441 cactccctgc ggattccgac gaagtcgccc acctattccg atttcaacgc gcccacggtt
     1501 ccgattttaa tccgcgcacc attccgagat atgcccgccc actttcggcg ccttgctggc
     1561 gaacaaagtt gaagctggtt tctggtttcg gtcaatcctt ctttgcggct cgcgctttgg
     1621 tcttgcgaag gctttcgcct gagatctcga cgcggtgtgc gttgtgaacg aggcgatcga
     1681 ggatggcgtc cgcatgggtc ggatcgccga tcacctcgtg ccattgctgg acaggaagct
     1741 ggctggtcac gacggtcgag cggcggccat agcgctcttc gaggatttcg agcagatcgt
     1801 ggcgcgcggt cggcattgag cggttcgagg ccccaatcgt cgagaatgag aagttcgacg
     1861 cgtgcgagcg tttcgaggag cctggcgtaa cggccgtcgc cgcgggccag cgccagttcg
     1921 gagaacagtt tcgggatgcg ctgataaagg gcggaccgca acagcatccg gcgctgagtg
     1981 ggtagcaaac gcctgttgac gtttcttgaa agcgcgttct aatgggggag cgtgtcctcg
     2041 tagctcagca ggatagagca ccagattcct aatctggggg tcacaggttc gaatcctgtc
     2101 ggggacacca ataaaatcaa tgagataacg gaagaacgcc ggagaacatt tgcgccgttt
     2161 tacaatttcg
//
LOCUS       DATMGX010000864         2991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_23130, whole genome shotgun sequence.
ACCESSION   DATMGX010000864 DATMGX010000000
VERSION     DATMGX010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23130"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            483..836
                     /locus_tag="VNR65_14170"
     CDS             483..836
                     /locus_tag="VNR65_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19863.1"
                     /translation="MPLENGRQGKDRQNPEKLSPLIDGGESVDMVAASDPGETAPEKA
                     PKASVDPVTPQEAAPAIKEEMMPKSPEELEQINKSYYAEASLTKKITVFAVTTVLILI
                     ALGLIYVNVPLGIVK"
     gene            complement(829..>2991)
                     /locus_tag="VNR65_14175"
     CDS             complement(829..>2991)
                     /locus_tag="VNR65_14175"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWJ19864.1"
                     /translation="EADQSPFYIAAAGAPSRPRCILKQNDCFAVLDNHGAIGCARDEA
                     GGLFARDTRHLSRLAFLINGQEPLLLGSTLRDDNLNLRADLTNPDILAGEGISLLKDT
                     VHISRTIYIHNGLLSARSAIVNHGNVPVSLDVTIDFESDFADLFEVRGMRRAARGILQ
                     KEVVGKNKVILRYRGVDNKVRETALTFDPEPTELKTTSARYSLTLPPSVPWRQFVTVS
                     CDGRMRPAAPSFLRGLLQARRDLVGNPHDIVTIETSNAVVNEIVCRSLADLRMLMTAT
                     PEGKYPYAGIPWYSTTFGRDGLITAMQTLWFDASVARGVLQRLAQFQAKTFDDASDAQ
                     PGKILHEMRSGEMADLGEVPFGLYYGSIDATPLFIILAGLYVERTGDLKFLRQIWPNI
                     VRGLDWIEAHGDSDGDGFVEYERATDKGLANQGWKDSHDAVFHADGELAEGPIALVEV
                     QGYVYAALLAAAKCARILGEHDRASDLVRKAERLQQRFEKAFWCEDLGTYALALDGKK
                     HPCKVRTSNPAHAMFTGIMAPDRARKVAQDIMRPSFYSGWGIRTVAAGEARYNPMSYH
                     NGSIWPHDNAIIASGLARYGLREGIEPIFNGMMQTAAYMDQRRLPELFCGFPRRRSRG
                     PTLYPVACSPQAWASGAVFQLLQAVLGLEYDLSARAIRLRNPAVPMSVGQITVRNMRL
                     GDAAISFTVRPDPNGTVSLGVLESSGKIKISVILDSST"
BASE COUNT          665 a          890 c          849 g          587 t
ORIGIN      
        1 agcccgtgcc gtcattgtag gcgagtgaaa aatacttatg taattactta taccatgttt
       61 acgaaacaaa accaatttta accgatcgta caccctgact ggatgacccc tggcttcgga
      121 aattcccctg gacggtcgac gtcgagaatt actcggcaag ccttccgatc gccgtagcca
      181 agacaaccgg ccacccaccg cgtttaggga tgcatgatcg gcttggtctg gggattgctt
      241 ctcctcacaa ccgaaagtct tcacactgga cgatcccgtc aggatcgtgc gctccttgag
      301 cgaatcggca gaggaaagca cgcggcgcaa agcacaaccc catcgctctg caatgtctat
      361 gctgaccttc tatatcccga acctcacaaa aaatactcga aaaagcgaag gatgaattgc
      421 gcctccttta tggtagagtg ccgcgcagaa agcggtcaca taaaaatgat taggatgctc
      481 ccatgcccct cgaaaacggc cggcaaggca aggataggca aaaccccgaa aaactcagcc
      541 ctctgatcga cggaggagaa agcgtggaca tggtcgccgc cagcgaccct ggagaaactg
      601 ctcctgagaa ggctccgaag gcgagcgtcg acccagtgac accccaagaa gcagctcccg
      661 cgatcaagga agagatgatg cccaaaagcc ccgaggaact ggagcaaatc aacaaatcgt
      721 actacgccga agcctccctc acgaaaaaga tcaccgtttt tgccgtgacc accgtcctga
      781 tcctgatcgc gcttggcctg atctacgtca atgtgccact tggcatcgtt aagtagagga
      841 gtccaaaatc accgaaatct tgattttgcc gctggactcc aacacgccaa gagaaactgt
      901 gccattgggg tccggccgca ccgtgaaact gatcgccgca tcgcccagcc gcatgttgcg
      961 cacggtaatc tgcccaaccg acatcgggac tgcgggattg cgtaaacgaa tggcccgcgc
     1021 cgatagatcg tactcaagac cgagaacggc ttgaagcaat tggaacaccg cgccgctcgc
     1081 ccaggcttgc ggcgagcacg ccacgggata gagcgtcggc ccgcggcttc ggcggcgcgg
     1141 gaatccacaa aacagctccg gtagccgccg ctgatccata taggcagcag tctgcatcat
     1201 gccgttgaaa atcggctcga tgccttcgcg gaggccgtag cgggcgagac cgcttgcgat
     1261 gatggcatta tcgtgcggcc agatcgaacc attatgatac gacatggggt tatagcgggc
     1321 ctcgcccgcg gccacggtcc gaatgcccca acccgaataa aatgacgggc gcatgatgtc
     1381 ttgcgcaact ttgcgtgcgc ggtcaggagc catgatgccc gtgaacatgg cgtgcgccgg
     1441 attgctggtt cgcaccttgc acgggtgctt tttgccgtcg agggcgagcg cgtaggtccc
     1501 gaggtcttcg caccagaaag ccttctcgaa gcgctgttgc agcctttcgg ccttccgaac
     1561 gaggtcactt gccctgtcgt gctcgccaag aatccgggcg catttcgcgg cagcaagcag
     1621 cgccgcgtag acgtagccct ggacctccac caatgcgatc gggccttcgg caagctcgcc
     1681 atcggcgtgg aagaccgcat cgtgcgaatc cttccagccc tgatttgcca gccccttgtc
     1741 ggtcgcgcgc tcatattcga cgaatccatc gccatcgcta tcgccatggg cttcgatcca
     1801 atcgaggccg cgcacgatgt tcggccagat ttgccgcaag aatttaagat ccccggtcct
     1861 ttctacataa aggccagcga ggataatgaa cagcggggtg gcgtctatgc ttccataata
     1921 gagaccgaag ggcacctcgc ccaaatccgc catttccccg ctccgcatct catgcaggat
     1981 cttgcccggc tgagcatcgg aggcgtcgtc gaaggtttta gcctggaatt gggcgagccg
     2041 ttgcagtacg ccgcgtgcga ccgacgcatc gaaccaaagc gtttgcatgg ccgtgatgag
     2101 gccatcgcgg ccgaacgtcg tcgaatacca tgggatcccg gcatagggat atttgccctc
     2161 tggcgttgcc gtcatcagca ttctgagatc agcgagagaa cggcagacga tctcgtttac
     2221 gacggcgtta gatgtttcga ttgtcacgat gtcatgcgga ttgccgacga ggtcgcgtct
     2281 cgcctgaaga agcccccgca ggaatgaggg agctgccgga cgcattctgc catcgcagga
     2341 cacggttacg aattgacgcc acggaaccga cggcggcagc gtcagggagt atctcgcgct
     2401 tgtcgtcttg agctcggtcg gctccgggtc gaatgtcagc gcggtttccc gcactttatt
     2461 atcaacgccc ctatatcgca gaatgacctt gttctttccg accacttcct tttgcaggat
     2521 tcctcgggcc gcgcggcgca tcccgcgcac ctcgaacaga tcggcaaaat cgctttcaaa
     2581 gtcgatcgtc acgtcgagcg acaccggcac gttcccgtgg ttgacgatcg cgctccgcgc
     2641 ggacaacagc ccgttgtgaa tgtaaatggt tcggctgata tgaaccgtat ctttaaggag
     2701 cgagatgcct tcgccggcga gaatatccgg gttcgtcaaa tcggcgcgca ggttgagatt
     2761 gtcgtctcgg agcgtcgagc cgagaagcag tggttcctgg ccgttaatga ggaaggcgag
     2821 gcgggacaga tgacgggtgt cccgggcgaa caagccgcct gcctcatcgc gagcgcaccc
     2881 tatggcgcca tgattgtcga gcacggcaaa acaatcattc tgcttcagga tgcagcgggg
     2941 ccgtgacggt gcgcccgctg cggcgatgta gaacggcgat tggtctgcct c
//
LOCUS       DATMGX010000865         2888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_8811,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000865 DATMGX010000000
VERSION     DATMGX010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8811"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VNR65_14180"
     CDS             <1..227
                     /locus_tag="VNR65_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19865.1"
                     /translation="LLGGVAEFSREELTILAESASLAEDFDIAGLRTQIEELEVGLAK
                     TPAGDELDRAIATLDHYKSILTSLTVTSAF"
     gene            267..584
                     /locus_tag="VNR65_14185"
     CDS             267..584
                     /locus_tag="VNR65_14185"
                     /inference="COORDINATES: protein motif:HMM:NF018993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HWJ19866.1"
                     /translation="MTTFDKREQGFEAKFIHDEELRFKAVARCNKMLGNWAAAQLGLT
                     GDAAATYANELVTAHLGSQTTDETLHKVSKDLAGKSISEEQVAQKMDAFLHLALEQIE
                     AGN"
     gene            604..915
                     /locus_tag="VNR65_14190"
     CDS             604..915
                     /locus_tag="VNR65_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19867.1"
                     /translation="MFEIQAQLVIVQKCEPESAAHSSGCKLVEQQAVAIVRIFESTTC
                     NVSLRPDSHRLDQLSGAMSIIRTAGIRKTRFSILRLPSVLTCTQVSNTGGLPIRLIAP
                     A"
     gene            complement(961..1341)
                     /locus_tag="VNR65_14195"
     CDS             complement(961..1341)
                     /locus_tag="VNR65_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWJ19868.1"
                     /translation="MRISKAIGLAIIVATSGLVFISSAQAASECPATGTLSSWGVNST
                     GYFSVASGGTCLFPIRMEGVVKSSSISQKPAHGTLKRLNISTYEYTAKAGYKGSDSFA
                     VQATGKGPTGLSGTSVITMNATVQ"
     gene            complement(1486..2142)
                     /locus_tag="VNR65_14200"
     CDS             complement(1486..2142)
                     /locus_tag="VNR65_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19869.1"
                     /translation="MPENGPLTRARLKTPKSAAIAGIVFSLMMFAIFWLLRQSIPADP
                     LNSGTWLTSSTRTIALALNLVPFAGVAFLWFIGVLRDRLGQHEDRFFATVFLGSGLLF
                     LAMLFAAAALIGAMMLLASAAEPNELINSATFRLARAATYIIMNVYAIKMAAVFMFST
                     STVVVYTDIAPRWIAYTGFLLALLLLIGSCYISWSFAVLPAWVLLISVYVLTDNLRAG
                     "
     gene            complement(2210..>2888)
                     /locus_tag="VNR65_14205"
     CDS             complement(2210..>2888)
                     /locus_tag="VNR65_14205"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HWJ19870.1"
                     /translation="RHQGTSAIVLAANLARLMAKAEHADGIIEFVPEVGDFVAIDEPL
                     FNLYGGAHSIDEDTLRASVVFGSERTMDQDPTFAFRIVVDIALKALSPAINDPTTGVL
                     AIDQLHRMLRQVGNRHLRTDEILDLCGQLRVVFRTPNWEDFVHLAFREIRACGSNNLQ
                     IVRRLRAMIENLVQTLPAHRHTALRSELDLLDREIAQNFLYPEDLALAHIADAQGLGG
                     HSGKIKH"
BASE COUNT          656 a          794 c          859 g          579 t
ORIGIN      
        1 tccttctcgg aggcgtggcg gaattttcgc gggaggagct tacgatcctg gcggagtcgg
       61 cctcgttggc cgaagacttt gatatcgctg gactgaggac gcagatcgaa gaactggagg
      121 tagggttggc aaagacgccg gccggcgatg agcttgatcg cgccattgcg acgctggacc
      181 actacaagtc aatcctcacg agcctgaccg tcaccagcgc cttctagctg cgtgcaacag
      241 atgcggaaat aatcgggacg gaaatcatga cgaccttcga caaacgcgaa cagggctttg
      301 aggccaagtt catccacgat gaagagctga ggttcaaggc ggtcgcaaga tgcaacaaga
      361 tgctgggcaa ctgggctgcg gcgcagctcg ggttgaccgg agatgctgcc gcgacctacg
      421 ccaatgaact cgtcaccgcg catttgggca gtcaaaccac cgacgagact ttacacaagg
      481 tatcaaagga cctcgcgggc aagagcatct cggaggagca ggtcgctcag aagatggacg
      541 cgtttctcca cttggcattg gagcagatcg aagccggcaa ttgacttcag gcgataggcc
      601 cgcttgttcg aaatccaggc gcaactcgtc atcgttcaga aatgcgaacc tgagtcggcg
      661 gcgcattcga gcggctgcaa gttggtcgag cagcaggctg tcgcaattgt caggatattc
      721 gagtcgacga cctgcaacgt aagtttgagg ccggattccc atcgcctgga tcagctcagc
      781 ggcgcgatga gcatcatcag aaccgcagga attcgcaaaa ctcgcttcag cattttgcgg
      841 ctcccgagtg tgcttacttg cacgcaagtc tcgaataccg gcggattgcc gatccggctc
      901 atcgctcccg cctaacgatt acgacccgct ggtttgcagc gggccggaat ctgtggaacg
      961 ctattgaacc gttgcgttca tcgtgatgac cgacgtgccg gagaggcccg tcgggccctt
     1021 gcctgtcgct tgaacggcga aactgtcgct gcctttatat ccggcctttg ccgtgtattc
     1081 gtaagtggag atgttgagcc gtttcagcgt cccgtgtgcc ggcttttgtg aaatgctcga
     1141 actcttcaca acgccttcca tcctgatggg aaacaggcag gtacctccgg acgcgacgct
     1201 aaagtagccc gtcgagttta caccccaact tgacagcgtt cctgttgcag ggcactccga
     1261 cgcggcctgt gccgacgaga tgaataccaa acccgaggtt gcaacgataa tcgccaaacc
     1321 gatagctttt gatattcgca tgctgtcctc caattttcag tttatgaaaa acacaagtag
     1381 acttctgctt cttgcgccct gcattggctt ggatcaaaaa cgccaatcga atttatcgac
     1441 atcgatatat cgacataatg ggtggagagt ggcgcctggc attctttatc ccgcccgcaa
     1501 attatccgtc aggacataga cgctgatcag taagacccaa gcgggcaata ccgcgaaact
     1561 ccagctgatg tagcagctgc cgatcagaag aagcagcgcc agcagaaatc cggtataggc
     1621 aatccagcgc ggtgcgatgt cggtatagac tacgaccgtc gacgtagaga acatgaatac
     1681 agcagccatt ttgatggcgt acacgttcat gatgatgtag gtcgcggcgc gtgcgaggcg
     1741 gaaagttgcc gaattgatta gctcattggg ctcggcggca gaggcgagga gcatcatggc
     1801 gcctatcagt gccgctgccg caaatagcat ggccaggaac agcaggccgc tgccgagaaa
     1861 aaccgtggca aagaagcggt cttcgtgttg accgagccga tcgcgcagca cgccgatgaa
     1921 ccagaggaag gcaacgccgg cgaacggtac cagattaagc gcaagcgcaa tggtcctcgt
     1981 actgctcgtg agccatgtgc cggaattgag cgggtcggcc ggaatggact gccgcagcag
     2041 ccagaagatc gcgaacatca tgagggagaa gacgatcccg gcgatggcgg cggatttcgg
     2101 ggttttcaga cgcgcgcgag taagggggcc attttccggc atcgccaaat tccttattgc
     2161 ttcagcgcag tcgacggctc agagttcagg ctgaacgcgc acgatgcgtt cagtgcttga
     2221 tctttccgga atgccctccc agaccctgag catccgcgat atgggcgagc gcaagatcct
     2281 ccggatagag gaagttttgc gcgatctcgc ggtccagcaa atccaattcg ctgcgcagcg
     2341 ccgtatgacg gtgcgccgga agcgtctgga ccaggttttc gatcatcgcc cgtaaccgtc
     2401 ttacgatctg caggttattc gaaccgcagg cgcggatttc gcggaacgcg agatgcacga
     2461 aatcctccca gttgggagtg cggaaaacca cgcgcaattg cccgcacaag tccaggattt
     2521 cgtcggtgcg cagatgacga ttgccgacct ggcgcagcat gcgatggagc tggtcgatcg
     2581 cgagcacgcc tgtggtcggg tcgttgatcg ctggggacag tgccttcagt gcaatgtcga
     2641 cgacgatgcg aaatgcgaaa gtgggatcct ggtccatggt gcgttccgac ccaaacacaa
     2701 ccgacgcccg caacgtgtcc tcgtcgatcg aatgagcgcc gccgtacaga ttgaacagtg
     2761 gttcgtcgat cgcaacgaaa tcgccgacct cgggcacgaa ttcgatgatg ccgtcagcgt
     2821 gttcggcctt ggccatgagc cgtgcgaggt tggccgccag aacaatggcc gatgttccct
     2881 gatgcctg
//
LOCUS       DATMGX010000866         3193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_436580, whole genome shotgun sequence.
ACCESSION   DATMGX010000866 DATMGX010000000
VERSION     DATMGX010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436580"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VNR65_14210"
     CDS             <1..793
                     /locus_tag="VNR65_14210"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWJ19871.1"
                     /translation="ACALIEAAEEKPDFDAVSKAVGMSRHHFHRVFKKTTGVTPGAYF
                     NARRRTRALDGLSRAGTVTEAIYAAGYSSSSRFYETCAPQLGLKPGAFAKGGAGEVIL
                     FAVGECSLGSILVAATARGICAIQLGDTLEELLEALQDRFPKADLKGGDADFEQIVAR
                     VVGLVEEPRRAFDLPLHVRGTAFQQRVWEILRSIPPGGTMTYSEVAAKAGCPAAVRAV
                     ASACASNKLAVAIPCHRVVRKGGALSGYRWGVERKAALLKRERAL"
     gene            790..1311
                     /gene="cobU"
                     /locus_tag="VNR65_14215"
     CDS             790..1311
                     /gene="cobU"
                     /locus_tag="VNR65_14215"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HWJ19872.1"
                     /translation="MSGASAPISLVLGGARSGKSAYAEKLIRAMPSPWLYCATAQALD
                     DEMRDRIAHHRARRGEGWITLETPLDLAEALAAATHPILVDCLTLWLTNVVLTGRDVP
                     AESAKLLAAAEVSKAPIVFVSNEVGLGIVPDNPLAREFRDHAGRLNQEVAKIASHVVF
                     MAAGLPMILKEEL"
     gene            1335..2387
                     /locus_tag="VNR65_14220"
     CDS             1335..2387
                     /locus_tag="VNR65_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWJ19873.1"
                     /translation="MFEKATSSPNDIDPRFYQRGGRLDVALEVYPNAPQPWIDLSTAI
                     SPWSYPYPPLADSEMHRYPNEVALQRLTKAAKKAYRAPAVADAVPVPGVNLGLSVLPW
                     FFREPKRVAVLAPEHSGHAAAWDAAGHSVSQIESMDRVGNAAILIVVNPSNVDGSVVP
                     HGDLAASLPPLKRRDGLLIVDETFADADPSHSLLPEVARLDYTLVLRSLGEFYGAAGI
                     GLGFAVTSHPITERLRSALGAWPVSAQAMAFGEAALADTVWAESQRARLKEAAKAMDA
                     ALEAAGLRILGGQALFRLAACTSGRQLFSHLASQGILTWPFQDRNALRFGLPRDEEEL
                     SRIRQALQSWCAISAD"
     gene            2687..3112
                     /locus_tag="VNR65_14225"
     CDS             2687..3112
                     /locus_tag="VNR65_14225"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HWJ19874.1"
                     /translation="MNAPAYDPDNIFAKILRREAPCHFVCEDQDTFVFMDIMPRSDGH
                     ALVIPKQPARNILDAPQGVLVPLIEMTQKVAIAAKEAFGADGVTVSQFSEPAGGQVVF
                     HLHVHVIPRYEGVALRSAGVMGDSALLAAHAEKLRAAMG"
BASE COUNT          590 a          930 c         1012 g          661 t
ORIGIN      
        1 agcttgtgct ttgatcgagg cggccgaaga gaagccggac ttcgacgcgg tctcgaaggc
       61 cgttggcatg agtcgccacc acttccatcg ggttttcaag aaaacgacag gcgtgacgcc
      121 tggagcttat ttcaacgcac gccgaaggac tcgcgcgctt gatggactaa gcagggcggg
      181 tacggtgacg gaagcgatct acgccgcagg ctatagctcg tccagccgct tttacgagac
      241 atgcgcgccg cagcttggtc tgaaacccgg cgcctttgcc aagggagggg ccggcgaggt
      301 catcttgttt gccgtgggcg aatgctcgct cggcagcatt ctagtggctg ccaccgcgcg
      361 ggggatatgc gcgatccagc ttggcgatac actcgaagaa ctcctcgaag ccttgcagga
      421 tcgctttccc aaggcggatc tcaaaggcgg tgacgcggac ttcgagcaga ttgtggcccg
      481 ggtcgtcggc ctcgtagagg agccaaggcg ggctttcgat cttccccttc atgtccgggg
      541 aaccgcgttc cagcagaggg tctgggagat cctgcgcagc attccgccgg gcggaaccat
      601 gacctattca gaggttgccg ccaaggctgg ctgcccggcc gcggttcgtg ccgttgcgag
      661 cgcctgcgcc agcaacaagc tggccgtcgc gatcccgtgc catcgcgtgg tgcggaaggg
      721 cggcgctctt tccggttatc gatggggtgt ggaacgcaag gcggcgcttc tgaagcgcga
      781 gagggcacta tgagcggagc gtccgctccg atttcgttgg ttcttggcgg ggctcgctcg
      841 gggaagagcg cctacgccga gaagcttatc cgcgccatgc cttccccttg gctttattgt
      901 gcgaccgcac aggctctcga tgacgagatg cgcgatcgca ttgcgcacca tcgggcacga
      961 cggggggagg ggtggatcac gctcgaaacg ccgctcgatc tggctgaggc gctggccgcc
     1021 gcgacgcatc cgatcctggt cgattgcctc acgctctggc tgacgaatgt cgtgctgacg
     1081 ggaagggacg tgccagccga gagcgcaaag ttgcttgccg cggcggaggt ctcgaaagcg
     1141 cccatcgtct ttgtctcgaa cgaggttggg ctcggcattg tgccggacaa tccattggcc
     1201 cgcgagtttc gcgaccatgc cggacggctc aaccaggagg ttgcaaaaat cgcgagccat
     1261 gtcgtgttta tggccgctgg cttgccgatg attttgaaag aagagctata actgccatta
     1321 gcgaggctga aaaggtgttt gaaaaggcta cttcctcgcc gaacgatatc gatccgcggt
     1381 tctatcagcg cggcggccgc ctcgatgtgg cgctggaggt ttatccaaat gcgcctcagc
     1441 cgtggatcga cctctcaacc gcgatcagtc catggagcta tccctatccc ccactcgccg
     1501 acagcgaaat gcaccgctat cccaatgagg tggcattgca gcgcctgacc aaggcggcaa
     1561 agaaggctta tcgggcgccc gctgtcgccg acgccgtccc agtgccgggg gtgaacttag
     1621 gattgagcgt gctgccttgg ttcttccgcg agccgaagcg cgtggcggtg ctcgcacccg
     1681 agcattctgg ccatgccgcg gcctgggatg cagccgggca ttccgtatcg caaattgaat
     1741 ccatggaccg ggtcggtaat gctgccatcc tcatcgtagt caatcccagt aacgttgatg
     1801 gcagcgtcgt cccccatggc gatctcgcag cttcattgcc gcccctgaag cggcgcgacg
     1861 ggctcttgat tgtcgacgag accttcgccg acgccgatcc aagtcattcg ctcctccctg
     1921 aagtggcgcg cctcgactac acgcttgtct tgcggtctct tggtgagttc tacggggcag
     1981 ccggtatcgg gcttggcttt gcggtcacgt cccatccgat aacggaacgg ctcaggtcgg
     2041 cgctcggggc atggcctgtt tcggctcagg cgatggcttt tggcgaagct gcgcttgccg
     2101 acacagtttg ggccgagtct cagcgggcaa ggttgaagga ggccgcgaag gcgatggacg
     2161 ccgcgcttga ggccgccggg cttcgaatcc ttggcggtca ggcgctgttc cggttggcgg
     2221 catgcaccag tggccgccaa ctattttccc acctcgcatc tcagggcatc ctgacgtggc
     2281 catttcagga ccggaacgcc ttgcgtttcg gactgccaag agacgaagaa gaattgtctc
     2341 gtattcgcca ggccctacaa tcctggtgtg cgatatcggc agattaaccg gcagcttttt
     2401 aggataggcc agtcctctac ccgaggtggc aaggctttgc acttggcgac tttcggtctc
     2461 agctatctaa gcgcttcgat gtgtaaaatc gaacgcagaa caaaacgagg gaataagctg
     2521 cgcgcagaac cgttctctta caaagcagca tgttgcccca cataatgtgc tgcttaaaga
     2581 ggcggcaagc tcaacgcgtg ccgtctcttt ttatgcctcc ccgcgcttga ccagttatga
     2641 cagtgtgggg cagaacagcg cggtctcaaa gcggaaggag atcgccatga atgcgcctgc
     2701 ctatgatccg gacaatattt tcgcgaagat tttgcgaagg gaggcgccct gtcacttcgt
     2761 ctgcgaggac caggacactt tcgttttcat ggacatcatg ccgcgttcgg atggccatgc
     2821 gctcgtgatc cccaagcagc ctgcgcgcaa tattctggac gctccgcaag gcgttctcgt
     2881 tccgcttatc gagatgacgc agaaagtggc cattgccgcg aaggaagctt ttggcgcgga
     2941 cggcgttacc gtgtctcagt tctcggagcc ggcaggcggg caggtggtgt ttcacctcca
     3001 tgttcatgtc attccccgct atgagggcgt ggctcttcgt tccgcaggcg tcatgggcga
     3061 cagcgccctt ctggcagccc acgcagagaa gctcagggct gcaatggggt gatttcgcgt
     3121 ttgcgttagg gcgagaagaa cctgagcccg atgatcgagc acacgagcgt cgcgaggaag
     3181 acgaagcgcg gca
//
LOCUS       DATMGX010000867         3656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_50317, whole genome shotgun sequence.
ACCESSION   DATMGX010000867 DATMGX010000000
VERSION     DATMGX010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50317"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            424..897
                     /locus_tag="VNR65_14230"
     CDS             424..897
                     /locus_tag="VNR65_14230"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWJ19875.1"
                     /translation="MDYLWTPWRYSYITAGEAPACIFCELLRSGDDEKSLIVYRGEHN
                     FVVLNAFPYTSGHSMIVPNEHLNRLHKLPSPAATEMMALSQRLETALIELYRPDGVNV
                     GMNIGKAAGAGIADHIHMHVLPRWVADANFMTVVGETRMLPELLETTYQRLKAKF"
     gene            complement(1198..1659)
                     /locus_tag="VNR65_14235"
     CDS             complement(1198..1659)
                     /locus_tag="VNR65_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19876.1"
                     /translation="MAKKIKTKSYEDAVAWFRGHGFDVLEAPGTSNRVFLKKYTCSAA
                     IERTEDGGVKVFAYPGYLMNGEISKLVDHGYQKVLKTTKAEHPATADHLKALHQFTEE
                     LKEGIGTISLYNEGLGTVSESYMYDRLRGRDLPEAERPKRPWQRAAGKRPA"
     gene            complement(2297..3469)
                     /locus_tag="VNR65_14240"
     CDS             complement(2297..3469)
                     /locus_tag="VNR65_14240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HWJ19877.1"
                     /translation="MNHRGAARLRGGHLWVYRSDLVPPAEAPAGSLVHVHDERGRRLG
                     SALYSSASQIAIRLLTGDEIAAAQLPSLLLERIAAAVRFRQQVVRDTDSYRAIFSEAD
                     LLPGLILDKYNDVLSMQALTQAFDRQDLRQTVLDAIAEHFPEASVIERVDAHIRELEQ
                     LPPRESQLVRGEQSSTIFTMNGVRFHFDGLGGQKTGAFLDQRENYAAAARYAHGDALD
                     CFTYQGGFALHLARTCGTVAAVDSSRPALEVAERNEQLNRAAHRCGEIEWIEANAFDL
                     LKDYATAGRQYDTIVLDPPAFAKTKRAVQTALRGYKELNLRALKMLRPGGILVTCSCS
                     FHVSEAEFLAMLGSAAADAARRVRILEERGQSLDHPMLLNVPETAYLKCVICQVLR"
BASE COUNT          739 a         1041 c         1088 g          788 t
ORIGIN      
        1 gcctgctggg tttgcggctg ctcgggagcg ccggagtctt gggtcggggt ggagggagcg
       61 ttgggggttt cttcaaaaga catgtggttg cgtatataca ctcgcgcgct ttaagaacac
      121 gcagagagcc ttataaaaac ggactgctgg tttgaatctt caggtgtgat tgcccgctta
      181 tagcgggttt ggacaaccca gcggccggat cctatggagc gagctccgct tccatggaac
      241 ttcttcgaat ataacaaccg tggcggagca gtgtcaaact tgtaagtgca atattcgcgc
      301 tgattttaca agctttttca ggatttttaa cctccctgtc aagtcggttg gatcaccggc
      361 cactgtgacg gcgacacgcc gaccgttagg gacgctaaga cggtttgata ctctgctcat
      421 ggtatggatt atctgtggac cccgtggcgt tacagctaca tcactgccgg cgaggcgccc
      481 gcttgcatct tctgcgaact gctacgctcc ggcgatgacg agaagtcgct gattgtctac
      541 cgtggcgagc ataacttcgt tgtcttgaat gcctttccgt acacctcggg ccactccatg
      601 atcgtgccca acgagcatct caaccgcttg cataagctcc cgtccccggc tgcaaccgag
      661 atgatggccc tgtcgcagcg cctggaaaca gccctcatcg agctctatcg ccctgatgga
      721 gtgaatgtcg gaatgaatat tggcaaagct gccggcgccg gcatcgcgga ccatattcac
      781 atgcacgttc tgccacgctg ggtggccgac gccaacttca tgacggtagt aggcgagacg
      841 cgcatgcttc cggaactgct ggagaccacg tatcagcgcc tgaaggcgaa gttctaggca
      901 gccgtcagct ttcagctctc agccttcaga atggatctca cccgccgccg gcactctttg
      961 cagagctggt gatggtaatt gctaattgct agagcggttt cgcaacagat gtatccggtt
     1021 tgtacgggta gtgcggcgct tcagcgccgc gttcgtcgcg gccaaacgtc cgctaaagcc
     1081 gctgaggtcg aagacctcag cggctttagc gattttcttc ctgcccctat tacgcgggcc
     1141 taaaggcccg cactacccgc acgggcggat acaccaattg tggaatcgtg tgggaaccta
     1201 tgcagggcgc ttacctgctg cccgctgcca cggacgcttg gggcgttccg cttccggcag
     1261 atcgcgtccg cgcaagcgat cgtacatata tgactcgctg acggtgccca atccttcatt
     1321 gtagagactg attgtgccaa tcccttcctt cagttcctcg gtgaactggt gcaacgcctt
     1381 gaggtgatca gccgtggcag gatgctcggc tttcgtagtc ttcaagacct tctgatagcc
     1441 atgatcgacg agtttcgaga tctcgccatt catgagatat cccggataag cgaatacctt
     1501 gacgccaccg tcttcggtgc gttcgattgc ggccgagcag gtgtacttct tcagaaacac
     1561 acgattactt gtgccgggag cttccaatac gtcgaatccg tgaccgcgaa accacgcaac
     1621 ggcgtcttcg tacgatttcg tcttgatctt cttggccatc ttcagcaata acccttcgag
     1681 aattaccctc gttcaacaga gccgcaatgt ccgctctcgc atcgaatgcg ataccggagt
     1741 accgcgcttg cgttgccttg gcggcaaaca aaattttcgc gagccggcat tccccagaga
     1801 ttcacactgt cgcacactgc accgccaacc ggcaacttag ggtgaggtca atgcggaacc
     1861 tgaatcagca tgattaacga acctcgaggc tttagcccct gcaaaaacag ttccctcagg
     1921 ggctgaagcc caagtcgttg ttgacccatt gcggcacgtc ggaagacctg ccctgataca
     1981 aaacgattta tgagctggct tctagcctgg attatttatg aatgtgaggt gtcatcctga
     2041 gcggagcgcc cgcaaagaat ttccgacccg cctcttttgc gggtcgggcg ggtgcggagt
     2101 cgaaggaccc ctatagctgc gagaaatcac agtgtggccg cgtgggcagc aacaagtttg
     2161 agatggctgg cggccaaagg gtccctcgac tcctcgctgc gctcgttgct cgggatgaca
     2221 cccaaaaaaa acgtgcgctc atgaataatc cggactaggg gtaacgtctt gcagtcaaaa
     2281 tttggaccaa cccttcttat cgcaaaacct ggcaaataac gcacttcagg taggccgttt
     2341 ccggcacgtt caatagcatt gggtggtcga gagactggcc gcgttcttcc agaattcgca
     2401 ctcggcgggc cgcatcggca gcggcggaac cgagcatcgc gaggaattcc gcctcgctga
     2461 cgtggaacga acaggagcag gtgacgagaa tgccgcctgg ccgcagcatc ttcagggcgc
     2521 ggagattcag ctccttatag ccacgcagag cggtctgtac ggcgcgcttg gtcttggcga
     2581 acgccggtgg atcgagaacg atagtgtcat actgccggcc cgcggtagcg tagtccttga
     2641 gcaggtcgaa agcgttggcc tcgatccatt cgatttcgcc gcagcggtga gcggcgcgat
     2701 taagctgctc gttccgctcc gccacttcca gcgccggacg cgacgagtcc acggcggcga
     2761 cggttccgca tgtccgcgcg aggtgcagcg caaatcctcc ctgataggtg aaacaatcga
     2821 gcgcgtctcc gtgagcgtac cgtgcggcgg cggcgtagtt ctcgcgctgg tcgaggaagg
     2881 cgccggtctt ctgcccgccg agtccgtcga aatggaagcg gactccgttc atggtgaaga
     2941 tggtgctgga ttgctcgccg cgcaccagtt gcgactcgcg cggcggcaat tgctccagtt
     3001 cgcgaatgtg ggcatcgacg cgctcgatga cgctggcctc gggaaagtgt tcggcgattg
     3061 cgtcgaggac cgtctgccgc aggtcctggc gatcgaacgc ctgggtcaac gcctgcatgg
     3121 aaagcacatc gttgtacttg tcgaggatga gcccgggcag caagtcggcc tcgctgaaga
     3181 tcgcgcggta ggaatcggtg tcgcgcacga cttgctggcg aaagcggact gcggcggcaa
     3241 tgcgttccag caggagcgat gggagttgtg ctgcggcgat ttcgtcgccg gtcaggaggc
     3301 ggatcgcaat ctgcgatgca ctgctgtaga gcgctgaacc caggcggcgc ccgcgctcat
     3361 cgtggacgtg gaccagcgag ccggcgggag cctcggcagg cggcaccaga tcggagcgat
     3421 agacccacaa gtgtccgcct cgcaggcgcg cggcgccgcg atggttcacg gttatgactg
     3481 gaatggaatc ggccagggcg tcctcgctag gaaatcaaac gcctatctta cgcgggatgg
     3541 tgggtcatga ctggtcagtc ctcccagatg caaaatggtc acaggggtgc atcttcaccg
     3601 ctctgtaaga ctccgccaat tcagttgtgc ggtgtgtcag ctaaatagaa tgacag
//
LOCUS       DATMGX010000868         2610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_60644, whole genome shotgun sequence.
ACCESSION   DATMGX010000868 DATMGX010000000
VERSION     DATMGX010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60644"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VNR65_14245"
     CDS             complement(<1..1122)
                     /locus_tag="VNR65_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWJ19878.1"
                     /translation="MPAVPGSEDVAVSVQTLIAELGVPRSAVQSGPLVVHSPITGEAM
                     ARVMPASGHEIDATIRAAHSAFLVWRDIPPPKRGELVRLLGHELRANKAALGRLVTIE
                     AGKIISEGLGEVQEMIDICDFAVGLSRQLYGVTIASERGEHRMMETWHPLGVTGIISA
                     FNFPVAVWAWNAALALVCGNACVWKPSEKTPLTALATQGLFDRALARFKEAGNPAPEG
                     LSSVIVGARDAGEALADHHLVPLVSATGSTAMGRALAPRLAQRFARAILELGGNNAAI
                     VCPSADLDLTLRAVAFAAMGTAGQRCTTLRRLFVHESIYDRFLPRLKLAYASVKVGNP
                     LEPETLVGPLIDGNAFAAMQQALEAATAAGGIVTGGARMT"
     gene            1252..>2610
                     /locus_tag="VNR65_14250"
     CDS             1252..>2610
                     /locus_tag="VNR65_14250"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWJ19879.1"
                     /translation="MSEANGVTFGPEELARSRVLQERFCTTFPEVFGDPEAPRTVVIV
                     PSITFDREVMSRIAGVHHYEERLLCLLLLLRFPRTRVIYITSTPIADPIIDYYLHLLP
                     GIPAQHARQRLVLLSCYDRSDIPLTAKILARPRLIQQIKEAIPDPASAHITFFTVTPL
                     EKKLALKLDLPIYGTDPDLAHWGSKSGSRKIFKEAGIELPAGSEDLTSADDVVEALTE
                     LKKQKPELKRAVVKLNEGFSGEGNALFDFRGAPESGLKAWIAKRLPDLNFEAHGMTLD
                     AYLEKIQLMGGIVEEFLEGETKRSPSAQFRVDPTGNLEVISTHDQVLGGGNSQVFLGC
                     RFPADAGYRLEIQALGFRAAEVLASKGVLGRFGIDFISVPEGSGWRHYAIEINLRKGG
                     TTHPFLMLQFLTAGSYDTKTGLFLTAAGKSCSYYSSDNLHEDHYRGLTPSDLIDIAAT
                     KGL"
BASE COUNT          527 a          830 c          766 g          487 t
ORIGIN      
        1 cgtcatgcgc gcgccgcctg ttacaattcc gcccgcggcc gttgcggctt cgagcgcttg
       61 ctgcatcgct gcaaaggcgt tcccgtcgat cagcggcccg accagcgttt ccggctcgag
      121 cggattgccg accttcaccg aagcataggc cagcttgagc cgcggcagga agcgatcgta
      181 aatgctctca tgcacgaaga gacgccgcaa agtggtgcag cgttggcctg cggttcccat
      241 cgccgcaaac gcgacggcgc gcagagtcag atcaaggtcg gcggacgggc aaacgatggc
      301 tgcattgttg ccgccaagtt cgaggatcgc gcgcgcaaaa cgctgcgcca gccggggggc
      361 aagcgcgcgt cccatcgccg ttgagccggt ggccgagaca agcggcacga gatgatgatc
      421 cgccagcgct tcgcccgcat cgcgcgcgcc aacgatgacg gatgaaaggc cttccggagc
      481 cgggttccct gcttccttga accgcgccaa agcccggtcg aacaagccct gcgtcgcaag
      541 cgcggtgagc ggtgtctttt cggatggctt ccagacgcac gcattgccgc acacaagcgc
      601 cagcgccgcg ttccaggccc agacggccac ggggaagttg aaagcggaaa tgatgccggt
      661 cacgcccagc ggatgccacg tctccatcat gcggtgttcg ccacgctcgc tggcgatggt
      721 cacgccgtag agttgccgcg agaggccgac ggcgaaatcg caaatgtcga tcatctcctg
      781 aacttcgccg agcccctcgg atatgatctt gcctgcctcg atggtgacga gccttcctag
      841 cgccgccttg ttcgcgcgaa gctcatgtcc cagcaagcgg acaagctctc cgcgtttggg
      901 cggcggaatg tcgcgccaga caaggaaagc ggagtgcgct gccctgatcg tcgcgtctat
      961 ctcatggccg ctcgcgggca taacgcgagc catggcctct cccgtgatcg gggaatgcac
     1021 gacgagcggg ccggactgga cagcggagcg cggcacaccc aattccgcga tgagagtttg
     1081 aacgctgacg gcgacgtctt ccgaaccggg aaccgctggc atgatcttgt cttttcctca
     1141 aaggctcaag cacggcatca atcggctttg cttgatccgt gtaagcaaac ccgttagctt
     1201 ttctaaatat agtgtgagac gcgttgataa gcgaaatatc ggggcaaggc cttgagtgag
     1261 gcgaacggcg taactttcgg cccggaggaa cttgcccgct cgcgagtcct ccaagagcgg
     1321 ttttgcacca cattccccga agtcttcggg gacccggaag caccgcgcac cgtcgtcatc
     1381 gtcccgagca tcaccttcga tcgggaagtc atgtcgagga ttgccggcgt ccatcactat
     1441 gaggaacgcc tgctttgcct tcttctcctg ctccggttcc cacgcacacg ggtcatttac
     1501 atcaccagca cgcccatcgc cgatccgatc atcgactact atctgcatct cttgccaggc
     1561 atcccggccc agcacgctcg ccagcgtctc gtgctgttgt cctgctacga caggtcggat
     1621 atcccgctga cggcgaaaat cctggcaagg ccacggctta ttcagcagat caaggaggcg
     1681 atccccgatc ctgcatcggc ccacatcacc ttctttacgg tcacgccgct tgagaaaaaa
     1741 ctcgcattga agctcgacct gccgatctac ggcaccgacc ctgatctcgc ccactggggt
     1801 tcgaagagcg gctcgcgcaa aatcttcaag gaagcgggga ttgagctgcc cgccggttcg
     1861 gaagatttga caagcgccga cgatgtcgtc gaagcgctga cggaactcaa gaaacagaag
     1921 cccgaactga agcgcgccgt ggtgaaactc aacgaaggct tctccggcga gggcaacgcc
     1981 cttttcgatt tccgcggcgc gccggagagc gggctgaaag cgtggatcgc aaagagactg
     2041 ccggacctca atttcgaggc tcatggcatg acgctggatg cctatctcga aaagattcag
     2101 ctcatgggcg gaatcgtcga ggagtttctg gaaggggaaa cgaaacgctc gccctcggct
     2161 cagttccgcg tcgacccgac cggaaacctc gaagtcatct cgacccacga ccaggtctta
     2221 ggcggcggta acagccaggt gtttctcggc tgccgctttc cggccgacgc gggctatagg
     2281 cttgagatcc aggccctcgg cttccgcgcc gccgaagtat tggcctccaa gggcgtttta
     2341 gggcgcttcg gcatcgactt catatcggtg cccgagggca gcggctggcg gcattacgcg
     2401 atcgaaatca acctgcgcaa gggcggcaca acgcatcctt tcctcatgct gcagtttttg
     2461 acagccggaa gctacgacac gaagacaggc ctttttctaa ccgccgccgg caaatcctgc
     2521 tcctactact catccgacaa tcttcacgaa gatcactatc gcggactgac gccttcggac
     2581 cttatcgata tcgccgcgac gaaaggcctg
//
LOCUS       DATMGX010000869         4851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_208688, whole genome shotgun sequence.
ACCESSION   DATMGX010000869 DATMGX010000000
VERSION     DATMGX010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208688"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4780)
                     /locus_tag="VNR65_14255"
     CDS             complement(<1..4780)
                     /locus_tag="VNR65_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWJ19880.1"
                     /translation="MGEVYRATDTKLGRDIALKVLPAEMAQDPERLGRFRREAKALAQ
                     LDHPNIVTIHSVEECGGIHFLTMQLVDGLPLDRLIPQGGLPVEQIIEIASALGDALAA
                     AHEKGIVHRDLKPANVMVSNEGRVKVLDFGLAKDVRGSNLGDATMTSASRTEVGVVMG
                     TPAYMSPEQTSARPLDHRTDIFSLGVMLHEMATGRRPFEGTSSAELVSSILRDTPPSV
                     TDVRPDLPSDLARIVRRCLEKDPRHRLQTARDVSNEFRDLARQTSQKVTPAKTSTPRT
                     VAAPDSGSVRADEGFWVAVLPFKCAGASAELKALADGLSEEIVTGLSRFSYLRVIARG
                     STAKYSSESGDVRTIGKELGARYVMEGSIRQAGSKLRLAVQLVDAVSGAHLWAENYER
                     AFSPEAVFELQDDLVPRIVSTAADMNGVLTRDMSEAVRGRAPEQLSPYEAVLRSFGYL
                     QRVTPEELATSLAGLERAVEKAPNYADAWAMLAYLCVQDYAQGVNLQADALTSGLIAA
                     RRAVEASPSNPLAYVSLAQVLFFHKEFQSFRNAAERAVVLNPMDGNSIAFLGELLTYA
                     GDRDRGLALAGSAKQLNPNHPGWYWYADFYNAYRQGDYSGARDFALKINLPGQWASHA
                     MLAAACSQLGEREAAAKAIRDLLHLRPNLGQTVRQEFKKWWDSEYVESVIDGLRKAGL
                     EMADDPAKRAPPASKASTTDSGAARADEGFWVAVLLFKYAGASAELKALADGLSEEVI
                     TGLSRFSYLRVIARGSTAKYSSESGDMRAIGKELGARYVMEGSLRLAGSKLRLAVQLV
                     DATTGTHLWAETYERAVQPESVFELQDDLVPRIVATVADRHGVLAHSMSEAVRAKNPT
                     QLTPYEAVLLSFGYAERLTSDEQAAAKACLERSVAQAPGNADAWAMLAMVFCDEYSIG
                     LKPHFSLMDQGLEAAKRAIKAGPSNHMAYNNLAYVHFLRREFGPCRHAAERALALNPM
                     DGSNVWFVGLMLAYMGEWDRGCALTERAMELNPHYPGKYRYPLVFNLYRKGDYKGALS
                     EALRLNMPDLFYTPLIVAAAAGQLGEEEIAAKAARDLLALRPDMAVDVRENLGKWFQP
                     EFVEHVVEGLVKAGLEIPPAQGSTVQHASPATSGLIKKRESGATRADEGFWVAVLPFK
                     STAASAELKALADGLSEEVVTGLSRFSYLRVIARGSTAKYSSESGDTRAIGKELGARY
                     VMEGSIRQAGAKLRLAVQLVDAVSGAHLWAETYERGFNTEAIFEVQDELVLRIVSTVA
                     DQHGVLPHSMANLIRNKPDDQLSPYEAVLRVFSFHERMSPEEHATVRALLERTVQSAP
                     SDGDCWAMLATLYSDEYMFGFSGRPDSLGRARAAAQQAVELAPSSSLAYQALAQSLFF
                     RREWQAFRPVAERTITLNRADGALHAFIGMLIALAGDWDRGCAVVDAAIHLNPHHPGW
                     YWLASVFGTYHKRDYRASADIALRVNMPGYFWGPATSAAALGQLGEHERAQKALKELL
                     VIRPDFARTARQEFGKWFDDELAEHYVEGLRKAGLQIVDSTQTSTTVRASGSGATRAD
                     EGFWVAVLPFRGASGDADLEALADGLTEDVTTGLSRFPYLQ"
BASE COUNT          943 a         1517 c         1450 g          941 t
ORIGIN      
        1 cttgaagata cggaaatcgc gaaagccccg tggtgacgtc ttcggtcaag ccgtcggcca
       61 gagcttcgag gtccgcatcg ccgctcgccc cccggaacgg caggacggca acccagaagc
      121 cttcatcggc tcgcgtggcg ccagagccgg acgctctcac cgtagtcgag gtttgcgtgc
      181 tgtccacgat ctgcagccct gctttgcgta ggccttcgac atagtgttcg gccagttcgt
      241 cgtcgaacca ctttccaaat tcctggcggg cggtcctggc gaaatcagga cgaattacga
      301 gcagttcctt cagagctttc tgcgcccgtt cgtgctcacc caactgccct aatgccgccg
      361 ctgacgtggc gggtccccag aaatagcccg gcatgttgac tctgagagcg atgtcggcgg
      421 aggcgcggta atcacgcttg tgataggtgc cgaaaacgga tgcgagccag taccagcccg
      481 ggtgatgcgg attgagatga atagcggcat ccacgacggc acagccgcgg tcccagtcgc
      541 cggcgagcgc gatcaacatg ccaatgaaag cgtggagggc gccatcggcg cgattcagtg
      601 tgatggttcg ttcggcaacg ggacgaaaag cctgccattc ccggcgaaag aacaagctct
      661 gggcgagagc ctggtaggct aacgaactgg atggcgcgag ttcgacggcc tgctgcgcag
      721 ccgcccgcgc tcgacccagt gaatccggcc ttccgctgaa accgaacata tactcgtcgg
      781 aatagagcgt ggcgagcatc gcccaacagt cgccgtcgct gggagcgctt tgcacagttc
      841 gctccagaag tgcccttacg gtggcgtgct cctccggcga catcctttcg tgaaagctga
      901 agacccgaag tactgcctcg tagggactca actgatcgtc gggcttgttg cggatcaggt
      961 tggccatgct gtgcggcagc acgccgtgct gatccgctac ggtggagacg atgcgaagca
     1021 caagctcgtc ctgcacttcg aagatggctt cggtgttgaa cccgcgctca tacgtttccg
     1081 cccagagatg agcgccggag actgcatcga cgagttgcac ggcaagacgc agcttcgctc
     1141 ccgcctgacg gatgctgcct tccatcacgt agcgcgctcc gagttcctta ccaatggcgc
     1201 gtgtgtctcc cgactcgctg gaatatttcg cggtcgagcc gcgggcgatc acgcggagat
     1261 aagagaagcg ggacagcccg gtgacgactt cttctgacaa gccgtcagcc agggctttca
     1321 gttcggcgct tgcggctgta gatttgaacg gcagcacggc gacccagaag ccttcgtcgg
     1381 cgcgcgtggc gccgctttcg cgcttcttga tcagcccact cgttgcgggt gaagcgtgtt
     1441 ggacggtcga tccctgagcg ggcggaatct ccagccccgc tttgacgaga ccttcgacga
     1501 catgttccac aaattccggc tgaaaccatt ttccaagatt ctcgcgtaca tccaccgcca
     1561 tgtctggcct caacgccagc aagtcgcgcg cggctttcgc ggcgatttct tcttctccaa
     1621 gttgcccggc ggccgctgcc acaatcaatg gcgtgtaaaa aaggtcgggc atattgagcc
     1681 gcagcgcttc gcttaacgca cctttgtagt cgcccttgcg gtataggttg aagacgaggg
     1741 gatagcggta cttcccggga taatgggggt tcagctccat tgctcgctcg gtgagtgcac
     1801 aaccgcggtc ccattccccc atataggcca gcatcaagcc cacaaaccag acattgctgc
     1861 cgtccatagg attcagcgcc agcgcacgct ccgcggcgtg tctgcaaggg ccaaactccc
     1921 ggcgcaggaa atggacatag gccaggttgt tgtaggccat atggttggaa ggcccggcct
     1981 tgatcgctcg ctttgcggct tccagcccct gatccatgag tgaaaaatgc ggtttgagcc
     2041 caatcgaata ttcgtcacaa aagaccatgg ccagcatcgc ccacgcatcg gcgttaccgg
     2101 gagcttgcgc gaccgaccgt tcgaggcagg ccttcgctgc ggcctgctcg tccgacgtga
     2161 gccgctcggc ataaccaaaa ctgagcaaga ccgcctcgta aggcgtcagc tgcgtgggat
     2221 tcttcgctcg caccgcctcg ctcatgctgt gtgctagcac accgtggcgg tcggcgacgg
     2281 tcgcaacgat ccgcgggacc aggtcatcct gcaactcaaa aaccgattcg ggttgaaccg
     2341 cgcgctcata cgtctccgcc caaagatgag tgccggttgt tgcatcaacg agttggacgg
     2401 cgagccgcag tttggatcct gccagccgta agctgccttc catcacataa cgcgcgccga
     2461 gttctttgcc gatggcgcgc atgtcgcccg attcgctgga atacttcgca gtagaacccc
     2521 gcgcgatcac tcgcagatac gaaaagcgag agaggcctgt gataacttcc tcagacaggc
     2581 cgtcggcgag ggctttgagt tctgcactcg cgcctgcgta tttgaacagc aacaccgcaa
     2641 cccagaagcc ttcgtcggca cgtgcagcgc cggaatcggt ggtgctggcc ttcgaagcgg
     2701 gaggcgcacg cttcgccgga tcatcggcca tctccagccc cgccttgcgg agtccgtcaa
     2761 tcacgctctc gacgtactcg gaatcccacc atttcttgaa ttcctggcgc acggtctgcc
     2821 caaggttggg ccgcaaatgc aggagatcac gtattgcctt cgcggcagcc tcccgttccc
     2881 cgagctggct gcacgccgcg gctagcattg cgtgggaagc ccattgtccg ggcagattga
     2941 tcttcagggc gaaatcgcgc gccccgctat agtcgccctg gcgatacgcg ttgtagaagt
     3001 cagcgtacca gtaccatccg ggatgattgg gattgagctg cttggcgctc cccgccagcg
     3061 cgagtccgcg gtcccggtcg ccggcataag tcagcaactc gcccagaaag gcgatggagt
     3121 tcccgtccat ggggttgagt acaacagccc gctcggccgc attccggaag ctctgaaatt
     3181 ccttgtggaa gaagagcact tgggcaagac tgacgtacgc cagaggattg gacggcgacg
     3241 cctcaacggc ccgccgcgcg gcgattagcc cgcttgtcaa ggcatccgcc tgaaggttga
     3301 ctccttgcgc gtagtcttgg acgcacaagt acgccagcat agcccacgca tcggcatagt
     3361 ttggcgcctt ctccaccgcc cgctccagac cagccagcga agtggcaagc tcttcggggg
     3421 taacccgctg caagtagccg aagctgcgca acactgcctc gtaggggctc aactgttcgg
     3481 gagcacgacc gcgcaccgct tcgctcatgt cgcgggtcag gactccgttc atatcagcgg
     3541 ccgtcgaaac gatccgcgga accaagtcgt cctgtaactc gaataccgcc tcggggctga
     3601 aggcgcgctc atagttctcg gcccagaggt gagcgcctga gaccgcatcc acgagttgca
     3661 cggcgaggcg cagcttcgat cccgcctgac ggatgctgcc ttccatcaca tagcgcgctc
     3721 cgagttcttt gccaatggtg cgcacatccc ccgactcgct cgaatacttc gccgtcgagc
     3781 ctcgcgcgat cactcgcaga taggagaagc gtgacaagcc agtaacgatt tcttcggata
     3841 atccatccgc cagcgctttc agctctgcgc ttgcgcccgc gcacttaaac ggcagcaccg
     3901 cgacccagaa gccttcatcg gcgcggacgg agccggagtc gggcgcggct acagtgcgcg
     3961 gggtggaagt ctttgccggc gttactttct gagaggtctg tcgcgccaga tcgcggaatt
     4021 cgttgctcac gtcgcgcgca gtctgcagac gatgacgagg atccttctcc agacagcgcc
     4081 gaacgatgcg agccaaatcg ctgggcagat caggacgcac gtcggtaact gacggtggag
     4141 tatcacgcaa aatcgaagag accagctcgg ccgacgatgt tccttcgaac ggcctgcgcc
     4201 cggtcgccat ctcgtgaagc atgactccga gagaaaaaat atcggtgcgg tggtcgagcg
     4261 ggcgtgccga tgtttgttca ggagacatgt aggctggcgt ccccatgacc acgcccactt
     4321 ccgtacggct ggccgaggtc atcgtggcgt ctccgaggtt ggagccgcga acatccttgg
     4381 caaggccgaa gtcgagcacc ttgacccggc cttcattcga aaccatgaca ttagcaggct
     4441 tcaggtcgcg atgcacgatg cctttctcat gtgccgccgc cagcgcatcc cccagagcgc
     4501 tcgcgatctc gatgatctgc tcgactggga gtccaccttg aggaatcagg cggtccagtg
     4561 gcagtccatc aactagctgc atcgtcagaa aatggatgcc gccgcattcc tccacagagt
     4621 ggatggtgac gatgttcgga tggtcgagtt gggcgagggc cttggcttcc cgacgaaagc
     4681 gcccgagccg ctccggatct tgagccatct cggcggggag cactttaagc gcgatgtcgc
     4741 gaccgagctt cgtgtcggtg gcgcgataca cttctcccat cccgcccgcg cctaacggag
     4801 actggatttc atatgggccg agtttcgtgc caggagtaag agccatccgt g
//
LOCUS       DATMGX010000870         6769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_390503, whole genome shotgun sequence.
ACCESSION   DATMGX010000870 DATMGX010000000
VERSION     DATMGX010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390503"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VNR65_14260"
     CDS             <1..739
                     /locus_tag="VNR65_14260"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWJ19881.1"
                     /translation="LSIYRDFGFEDVIIKLSTRPEKRVGSDEIWDKAEAALKVTLDQI
                     GHPYSISPGEGAFYGPKLEYTLRDAIGRHWQCGTVQLDFNLPERLGAFYIGEDGAKHT
                     PVMIHRAMFGSLERFIGILTENYAGHLPLWLAPIQVMVATIVSDADPYAGRVLEACEK
                     AGLRSETDLRNEKINYKVREHSLAKIPVMFVVGKREAEEQTVSIRRLGSQAQETLPLT
                     EAIKRISAEAIPPDLAREKEPRKREAA"
     gene            1323..2582
                     /locus_tag="VNR65_14265"
     CDS             1323..2582
                     /locus_tag="VNR65_14265"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWJ19882.1"
                     /translation="MVELANALVTVNTPRLPARVRGRYTEIPKNALFSSLSRQQIRLV
                     LPHVKHKRFKSGRVILQEGTKNPGKIYIIMEGQVALTKEGLSPLESLPTCYELGILRR
                     GEIFGEMSFVDGKPSAVSFMTKSETVVAVVDLSGSRRRSTTKRLRDVVANKLRHHLTR
                     HADESMTLRVNTLQLENEFSTYRNGVGHIVVATLCLLSFYTLTLSFLPSFKSLAHANF
                     ALTPVVILLFGLCFVPIIATSGFPLKFFGLRFDNWRPALAYSLRASLFFMAIFVAAKW
                     VLISTSDSLAGVSLFDSADVEVSGHPVPMTAWYWLALAVYVLLTPMQEFVARSGIQAP
                     LYAFLQGSELKRRWFSILASNLVFAAAHAHISLAFAIAAFLPGMLWGWIFARTNSLLA
                     ATASHLLVGGMGMFLFGIEGLISRLSA"
     gene            complement(2587..2910)
                     /gene="yidD"
                     /locus_tag="VNR65_14270"
     CDS             complement(2587..2910)
                     /gene="yidD"
                     /locus_tag="VNR65_14270"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWJ19883.1"
                     /translation="MTSPLSASNTILKAAAKSPVYFYRYVISPILGPRCRHLPTCSQY
                     ALDAIDVNGVWIGVWLSLGRIGRCHPWGTHGYDPAPDLRQARIPFWAPWRYLRYYPRR
                     EKTEA"
     gene            complement(2903..4366)
                     /locus_tag="VNR65_14275"
     CDS             complement(2903..4366)
                     /locus_tag="VNR65_14275"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWJ19884.1"
                     /translation="MTIHQRFRLIIGVGLFTILCVAGASRSFAMDARSDLSFADIAAH
                     VKNSVVTVAAAAVDARQAGRLNRGRESRAEGGESQSFEDFSPLRPKRGPGEPVRQFTS
                     IGSGFIIDPSGLIVTNNHVIEGGNAVYVILSDGSELKVDKVIGRDPKTDVTLLKVTPK
                     ASNPLTAVPFGDSGRMRVGDWVIAIGNPFGLNGTVTAGIVSGRGRDINAGPYDDFIQT
                     DASINRGNSGGPLFNAVGEVIGINTAIFSPSGGSIGLGFAIPSNTAQRVIDQLKRYGE
                     TRWGWIGVRLQTPSDDLASGFHLASASGALIARVDKGGPADIAGLLEGDLVLSFAGSE
                     VKHARQLPRIVAQAPVGEETEIVILRGGERKTLKVKVGLLEEHQGPAASSPHGKPRRH
                     KISFQPLSNENRDQFSLAGDVEGAVVAGAQAQDVTNGQLRPGDLIIEAAHQRVRNLED
                     LENRLAELQRLSRSEALLTIQDRDGGIRFASLPLGDD"
     gene            complement(4522..5466)
                     /gene="hflC"
                     /locus_tag="VNR65_14280"
     CDS             complement(4522..5466)
                     /gene="hflC"
                     /locus_tag="VNR65_14280"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HWJ19885.1"
                     /translation="MRAGILGFLILTVIAAATAVGFSAFIVNQTHRALVLQFGEPVRA
                     IDEPGLYWRTPFIQTVEQFDRRILDLQTDEQEVIASDQKRLIVDAFARYRITDPLAFY
                     KAFRSEVGARQRLTAIVDSTIRSVLGRATFIDVVRNKRDALMKQTIKQVNDDVGNLGV
                     EIVDVRIRRADLPEANSQAIYRRMQTERQREAAELRAQGSEQSQRIKSTADKEVTVLI
                     ANANRDSERLRGDGDAERNHIYADAFSRDKDFFAFYRSMQAYEEALKGSHTRIVLSPS
                     SEFFRYFNGGGPKPGDGVKAPPPEPTGSSAAASTPPVR"
     gene            complement(5463..6449)
                     /gene="hflK"
                     /locus_tag="VNR65_14285"
     CDS             complement(5463..6449)
                     /gene="hflK"
                     /locus_tag="VNR65_14285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HWJ19886.1"
                     /translation="MPGGIGVFGSLLIFLVVAAAVGYFGFTVRINPDERGVVQRFGKY
                     DRELYNGINFRWPYPVEEVTVIPYTRQNRIEIGFSSGAGGPFGQVRAPNRSEESLMLT
                     GDENIVDVNFSVFWNVKDAAAFLFNVRNQGESLDANANVKAVAEAAMREVIGQNEIQP
                     ILTKSRQEVEESVKGLLQRTLDSYKAGINITQVVLQKVDPPAEVISAFRDVQAARADQ
                     ERARNEAEAYANRVIPEARGDAQRVLQAAQGYREQSVAEAKGRTDRFLKVYDEYQKAP
                     DVTRRRIYIETLERVLGGMDKLIIDEKTSASGVVPYLPLGDLQKSGQSGGQR"
     gene            complement(6468..6587)
                     /locus_tag="VNR65_14290"
     CDS             complement(6468..6587)
                     /locus_tag="VNR65_14290"
                     /inference="COORDINATES: protein motif:HMM:NF023642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK N-terminal
                     domain-containing protein"
                     /protein_id="HWJ19887.1"
                     /translation="MPWNQNSGGNWKGGPGGPWGQGPSGGGQQPDVEEILRRS"
BASE COUNT         1383 a         1942 c         1958 g         1486 t
ORIGIN      
        1 cctgtcgatc tatcgcgatt tcggcttcga ggacgtgatc atcaagctgt cgacgcggcc
       61 cgagaagcgg gtcgggtcgg acgagatatg ggacaaggcc gaggccgcgc tcaaagtgac
      121 gttggaccag atcgggcatc cctattcgat aagtccgggg gagggcgcgt tctacggtcc
      181 gaagcttgaa tacacgctcc gtgacgccat tggccggcac tggcagtgtg ggacggtgca
      241 actcgacttc aacttgcccg agaggcttgg cgccttctac atcggtgagg atggcgcgaa
      301 acatacgccc gtgatgatcc acagggccat gtttggcagc ttggagcgct ttatcggcat
      361 tctcaccgag aactatgccg gtcaccttcc gctgtggctt gccccgatac aggtaatggt
      421 ggcgaccatc gtctcagacg ccgatcccta tgcgggcagg gtgcttgagg cttgcgagaa
      481 agccgggctt cgctctgaga ccgaccttcg caacgagaag atcaactaca aggtccgcga
      541 acatagcctt gcgaaaattc ccgtgatgtt tgtggtgggt aagcgcgagg ccgaggagca
      601 gacggtttcc atccggcggc tcggcagcca ggcgcaagaa acactcccgc tgactgaagc
      661 gattaagcgg atatctgccg aggccatccc cccggatctt gcccgcgaga aagagccgcg
      721 caaacgggaa gccgcctaat ataacgagcg ataatgtgag aggggctggc ggctctcgcc
      781 gtcagcctgg gctttgccgt taaccaaagt gacacacttt ccaaccaagc acccgcttca
      841 cgggattttg aaacaggtgt gccgaagaat ttcagctatc attcacaatg agattgagag
      901 aatcgagccg aagcatttca aggaatgaag ggcggttcaa aaaactagag aagttaagag
      961 tcgtaaaact aaagtggtac tactttcagt ccaatgcaat gatgaagtgg catgcatagc
     1021 ctcgtctcaa gcggaattaa aagaaactga aggcttcgtt agcttaatat ttgcccagca
     1081 aacactatga tccttctctc ttttactggt atcatggcga ggaaacggga actaggcgta
     1141 tgggcggtag cgggcgagct gtgcagttga gagggaattc cttcgactcg ctgtacacat
     1201 aatcttgtac cttggaacag catagcagga atgtccgctg gatcgtttaa ccagcgcaag
     1261 gtttaacgtg catatacagg agctaaagtt aaactgaaac tcgggatcgg cctcgaaagt
     1321 ccatggttga attggcaaac gcgctagtta cagtaaatac acctcgctta ccggcgcgcg
     1381 ttagaggccg ttatactgaa atacccaaaa acgctctgtt ttcaagtctg tctcgtcagc
     1441 agataagact tgttcttcct catgtaaagc acaagaggtt caagtccggg cgcgtgattc
     1501 ttcaggaagg cacgaaaaac cccggcaaaa tctatatcat tatggaaggg caggtggccc
     1561 tgacaaagga aggtctctct ccgcttgaga gcctgcctac ctgctacgaa ctcggtattc
     1621 tccgccgcgg cgagatcttt ggcgaaatgt ctttcgtcga tggtaagccg agcgccgtct
     1681 ccttcatgac gaagtccgag acggttgttg cagtcgtcga tctgagcggg tctcgccgga
     1741 gaagcaccac aaaaaggttg cgtgacgtgg ttgccaacaa gctccgccac catctcacga
     1801 ggcacgccga tgaatccatg acgcttcgcg tcaatactct ccagctcgag aacgaatttt
     1861 cgacttatcg caatggcgtg ggccacatcg tggttgcaac gttgtgcctt ttgtcgttct
     1921 acacgctcac gctaagcttc ttgccaagct tcaaaagcct ggctcatgcg aatttcgcgc
     1981 ttacccctgt ggtgatcctg cttttcggcc tctgcttcgt tccgattatt gccacgagcg
     2041 gctttcctct gaagtttttc ggccttcgct tcgacaactg gcgtccggcg ctcgcttatt
     2101 cgctgcgcgc ttctctgttt ttcatggcga ttttcgtggc tgccaaatgg gtgctgatca
     2161 gcacttcgga cagccttgcc ggcgtttcgc tcttcgacag tgcggatgtg gaggtgagcg
     2221 gccatccagt accgatgacg gcttggtatt ggcttgccct tgccgtctat gttctgctta
     2281 ccccgatgca agagttcgtc gctcgctcgg gcattcaggc gccgctctat gcgtttctgc
     2341 aggggagcga actgaagcgg cgctggtttt cgatcctggc ttcaaacctc gttttcgccg
     2401 ccgcgcatgc gcatatcagc cttgcctttg cgatcgcggc ctttctcccc ggcatgctct
     2461 ggggatggat cttcgcgcga acaaactcgc ttctcgccgc aacggcttcg catctgcttg
     2521 tcggcggaat ggggatgttt ctcttcggaa tcgaaggctt gatttccagg ctttcagcct
     2581 gacctttcaa gcttcggtct tttcccggcg cggataatat ctcagatacc gccaaggcgc
     2641 ccagaagggg atcctggcct gtcgaaggtc gggggcaggg tcatagccat gggtgcccca
     2701 agggtggcag cgaccgatac ggcctagaga cagccagacg ccaatccaga ccccgttcac
     2761 atcgatggcg tcgagcgcgt attgcgagca ggtgggcaaa tggcggcagc gcggacccag
     2821 gatcggtgag atcacgtaac ggtagaaata cacgggcgac ttcgccgctg ccttcagaat
     2881 cgtgtttgag gctgataagg gactagtcat cgccgagcgg tagcgaggcg aagcgaattc
     2941 ccccatcgcg gtcttgaatg gtcaggagcg cttcggaccg cgacaggcgt tgcagttccg
     3001 ccagccgatt ctcaaggtct tccaggttgc gaacacgctg atgcgcggct tcgatgatca
     3061 aatccccagg ccgcagctgc ccattcgtaa cgtcttgggc ctgcgccccc gctacaacgg
     3121 ctccttcgac atcgccggca agagagaact gatcacggtt ctcattcgac aaaggctgga
     3181 agctgatttt gtgacgcctg ggcttgccgt gcggagagga cgctgccggc ccttgatgct
     3241 cctccaggag cccgaccttc acttttaagg tcttgcgctc gccaccccga aggatcacga
     3301 tttcggtctc ctcgccaacc ggcgcctgcg cgacgatgcg gggaagttgc cgggcatgtt
     3361 tcacctctga tcccgcaaag gaaagaacga gatcgccctc caaaaggccc gcaatatccg
     3421 caggtccacc cttgtccacg cgggcgatca gtgctcctga ggcgctggcg agatgaaagc
     3481 cgctcgcgag atcgtcgctt ggggtctgaa ggcgcacgcc gatccagccc cagcgggtct
     3541 cgccataacg cttgagctga tcaatgacac gttgcgctgt gtttgagggg atggcgaagc
     3601 cgagcccgat gcttcctccg gatggagaga agatcgccgt gttgatcccg atcacttcgc
     3661 ccacggcatt gaagagcggc ccgcctgaat ttccacggtt gatggacgca tccgtctgaa
     3721 tgaaatcgtc ataaggcccg gcattgatat cgcgtccgcg tccggaaaca atgccggcgg
     3781 tgacggtgcc gttgaggccg aaggggttgc cgattgcaat gacccagtct ccgacgcgca
     3841 tccgccccga gtcgccaaag ggcacggcag tcagcgggtt cgaggcttta ggcgtgacct
     3901 tgagaagtgt gacgtcggtt tttgggtcgc ggccaataac tttatcgact ttcagctccg
     3961 acccgtcgga gaggataacg taaacggcgt ttccgccctc gatgacgtga ttattggtaa
     4021 cgataagtcc gctcgggtcg atgatgaacc cggagccaat cgaggtaaat tgacggaccg
     4081 gctcgccggg acctcttttc ggccggagcg ggctgaagtc ttcgaaagac tgcgattcgc
     4141 cgccctcggc gcggctctcc cggcctcggt tgagccggcc ggcctgtctc gcgtcaaccg
     4201 cggcggccgc aaccgttaca acggaatttt tcacatgtgc cgcgatgtcg gcaaaggata
     4261 gatcggatct tgcgtccatg gcgaatgacc gtgaggcacc cgcgacgcat aatatagtaa
     4321 aaaggcccac gccgatgatc agccggaacc gttggtggat cgtcattagg gcctcagcca
     4381 ggagaacatg aaaacaggac tgcaaaagac gcctcgtcaa acgcagccca ttataaggtg
     4441 gggtataaac gtggcgagcg caatctcgct aattgccgat agcagacaaa ggttaactat
     4501 cttgctttgc gcctcaatct atcagcgcac cggcggggtg gaagcagccg cgctcgaccc
     4561 ggtcggctcc ggtggtggcg ccttcacacc atctcctggc tttggccctc ctccgttgaa
     4621 ataccggaag aactcggagc tcggcgaaag cacgatgcgc gtatgcgatc ccttcagagc
     4681 ttcctcataa gcctgcatcg agcgatagaa tgcgaagaaa tccttgtccc gggagaaggc
     4741 gtccgcataa atgtggttcc gttccgcgtc gccgtcgccg cgcagtcgct cggagtcgcg
     4801 gtttgcgttg gcgatgagca cggtcacttc cttatctgcc gtcgatttga tgcgctggga
     4861 ttgctcgctg ccttgtgccc tgagttcggc tgcctcgcgc tgacgttccg tctgcatccg
     4921 ccggtaaatc gcttggctgt tggcctcggg caaatcggcg cgtcttatgc gcacgtccac
     4981 gatttcgacg ccaaggttgc caacatcgtc gttgacctgc ttgatcgtct gcttcatgag
     5041 cgcgtcgcgc ttattgcgga cgacatcgat gaaggtcgcc ctgccaagca cgctccgaat
     5101 ggtggaatcg acaatggcgg tcaggcgctg acgggctcca acctcgctgc ggaaggcttt
     5161 atagaacgcc agggggtcgg taatccggta gcgggcgaag gcatcgacga tcaggcgctt
     5221 ctgatcggag gcgatgactt cctgctcgtc cgtttgcaga tcaagaatcc gacggtcgaa
     5281 ttgctccacc gtctggatga agggggtgcg ccaatacagg cccggctcgt caatagccct
     5341 gaccggctcg ccaaactgca gaacgagcgc gcgatgggtc tgattgacaa tgaaggcgga
     5401 aaacccgacg gccgtggcag ccgcgataac ggtcaggata aggaatccta aaatgcccgc
     5461 tctcatcgct ggcctcccga ctgcccggac ttttgcagat caccgagggg gagatagggg
     5521 acaacgccgc tggcgcttgt tttttcgtcg atgatcaatt tgtccattcc acccagcacg
     5581 cgctctagcg tctcgatata gatccgccga cgcgtcacat cgggcgcttt ctgatattca
     5641 tcatagacct tcaggaagcg atccgtccgg ccctttgcct ccgccaccga ttgttcccgg
     5701 tagccttggg ccgcctgcaa aacacgctgc gcatcgccgc gcgcttccgg aatgacgcgg
     5761 ttcgcatagg cttccgcctc gttgcgggcg cgttcctggt cggcgcgggc ggcctgaaca
     5821 tctcggaacg ccgaaatcac ctctgcgggc gggtccacct tctgaaggac gacctgcgtg
     5881 atgttgatcc cagctttgta gctgtcgagg gtgcgctgaa ggagaccctt caccgactct
     5941 tcgacttcct ggcggctctt tgtcaggatc ggctgaattt cgttctggcc gatcacttcg
     6001 cgcatcgcgg cctcggcaac ggccttcacg tttgcatttg cgtcgaggct ctcgccctgg
     6061 ttgcgaacgt taaaaagaaa ggcggccgca tccttcacgt tccaaaacac gctgaaattg
     6121 acatcgacga tattttcatc cccggtcagc atcaggcttt cctctgaccg gttcggggcc
     6181 ctaacttgtc cgaacgggcc acctgcaccg gagctgaaac cgatttcaat ccggttctga
     6241 cgtgtgtagg ggatgactgt cacttcctcg acggggtagg gccatcggaa gttgatgccg
     6301 ttatacaact cgcggtcgta cttgccgaaa cgctggacaa cgccccgctc gtcaggattg
     6361 atgcggaccg tgaatccgaa atagccgacg gcggccgcga cgacgaggaa aatcagaagc
     6421 gagccgaaaa ccccgatgcc gccaggcatg gcttggcgaa gcttatccta gctgcgccgg
     6481 aggatttcct ccacatcggg ctgttggccg ccgcctgacg ggccttgacc ccaggggccg
     6541 ccgggaccgc ctttccagtt gccgccgcta ttctgattcc acggcatttt gtgattcctt
     6601 cgcaatcggc aggatcgccg ctttcgggcc gcccgcttgg ctattcatca ggtctgtaaa
     6661 aggatgtcaa tgacggtcac gatttgacaa atctttgtct gtcgcgagat atcggcgttc
     6721 atatttaagg aataatgaat gccccgtttc ccgatgtgtc tcgcggata
//
LOCUS       DATMGX010000871         3260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_116454, whole genome shotgun sequence.
ACCESSION   DATMGX010000871 DATMGX010000000
VERSION     DATMGX010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116454"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VNR65_14295"
     CDS             <1..551
                     /locus_tag="VNR65_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWJ19888.1"
                     /translation="ASESSRANAKPGDISDGKPLVVLINGGAASASEIVAGALQDHKR
                     AEVVGTRSFGKGSVQTMIPLGANGGLRLTTARYYTPSGRSIQATGISPDYLVEPELPD
                     DLKAQMAALTPKGEASLRGHLKNDSAPEAKGSISYVPKEKEKDVQLKAAIDLLHGKKI
                     ETTKKLENAAAPASAEPAEVPN"
     gene            637..945
                     /locus_tag="VNR65_14300"
     CDS             637..945
                     /locus_tag="VNR65_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19889.1"
                     /translation="MMKALLIVAGLFFSIFGAVIAYFAASDPGQEYELKFVLPIDVRQ
                     MPKAVAPPSVQSWSAGQEESAAATDGRAEAGKAPAMPDRPPVEFGERSGAASESQLRR
                     "
     gene            1040..1543
                     /locus_tag="VNR65_14305"
     CDS             1040..1543
                     /locus_tag="VNR65_14305"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HWJ19890.1"
                     /translation="MPLIDPKTLPYRPCVGIMLLNLEGLIWIGRRSDGLVSHEFQQPW
                     QMPQGGIDKGEDPRVAAMRELQEETGVTSASIIAESKRWIQYDLPPETIGVAMKGKFR
                     GQEQKWFAMRFTGDEREINLNPEGHKPEFDAWRWAAASEVLDEIVGFKRDAYRAVIEE
                     FRPLLAP"
     gene            1731..2288
                     /locus_tag="VNR65_14310"
     CDS             1731..2288
                     /locus_tag="VNR65_14310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004406.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HWJ19891.1"
                     /translation="MASGGRESTVAERYAGALFNLAHESNSVESVERALDKFLGLLRD
                     SADLRRFVLSPMFSAVEQEQGLSAILKSAGITGLARDFLLVLAKNRRLFATENVIKTY
                     KSLCAKSRGEVEAEVISAASLTQAQTQDLTDTLRQKLGKTPTLTAKVDPRLLGGLIVR
                     VGSQMIDTSLRTKLKNLEKLMKEAS"
     gene            2297..>3260
                     /gene="atpA"
                     /locus_tag="VNR65_14315"
     CDS             2297..>3260
                     /gene="atpA"
                     /locus_tag="VNR65_14315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWJ19892.1"
                     /translation="MEIRPSEISSILREQIKNFGQEAEVSEIGQVLSVGDGIARVYGL
                     DNVQAGEMVEFPGGVRGMALNLEADNVGVVIFGDDRNIKEGDTVKRTGAIVEVPVGKG
                     LLGRVVDALGNPIDGKGPIVADKRARVDVKAPGIIPRRSVNEPRATGLKAIDSMIPIG
                     RGQRELIIGDRQTGKTAIALDTILNQKALNQSGDESKKLYCVYVAIGQKRSTVAQFVK
                     VLEEHGALEYTIVVAATASDPAPMQYLAPFSGCAMGEFFRDNGMHAVIIYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKVSDRLGGGSL"
BASE COUNT          698 a          974 c          978 g          610 t
ORIGIN      
        1 atgcgagcga gagcagccgc gcaaacgcca agccgggtga catctctgat ggaaagccgc
       61 tcgtggtgct catcaatggc ggggcggctt cggcgtcgga gatcgttgcg ggcgcgcttc
      121 aggatcacaa gcgcgcggaa gttgtcggca cacggtcctt cggcaaaggg tccgttcaaa
      181 ctatgatccc gcttggagca aatggcggcc tgcgcctcac gaccgcccgc tattacacgc
      241 cctcgggccg ctcgatccag gccacgggca tctcgccgga ctatctcgtc gagcccgagt
      301 tgccggacga tttgaaagca cagatggcgg cgctcactcc taaaggggaa gccagcctgc
      361 gcggtcacct taagaatgac agcgcccctg aggcgaaggg cagtatctcg tacgtcccga
      421 aagagaaaga gaaggacgtt cagcttaaag cagcgatcga tctcctgcac ggaaagaaga
      481 tcgagacaac gaaaaagctg gaaaacgctg cagcgccagc ctcggctgaa ccagcggaag
      541 tgccaaatta gactttcgca accgtcatca cccctctata aaaggctctt gtcgcaagac
      601 tagggccttt tatttggccg ggctcagagg agtggaatga tgaaagcgct tctgattgtg
      661 gcagggctgt ttttctcgat cttcggcgca gtgattgctt atttcgccgc ctccgatcca
      721 ggccaggagt acgagctcaa attcgttctg ccaatcgatg tgcgtcagat gccaaaggcc
      781 gtcgccccgc cttccgttca gtcgtggagt gccggccagg aggagagcgc tgccgccacc
      841 gatggacgcg ccgaggctgg gaaagcgccg gccatgccag atagaccacc cgttgaattt
      901 ggcgagcggt ccggcgccgc atccgagtcg caactgcgga gatagccgaa cagaggagcg
      961 gccgtttcgc tcaacttttt caccagtttc gcggcagaca ccagccgcgc tttcgtccat
     1021 gctgcaaatt ggattggcaa tgcctctcat agaccccaaa acgcttcctt accgcccctg
     1081 cgtcggcatc atgctcctga acctagaggg gctcatctgg attggccggc gctccgacgg
     1141 tctcgtatcg cacgaattcc aacagccatg gcaaatgccg cagggcggaa tcgacaaggg
     1201 ggaagacccg agagtcgccg ccatgcgtga gcttcaggaa gagaccggcg tcaccagcgc
     1261 aagcatcatc gccgaatcga aacgctggat tcagtatgat cttcctcccg aaactatcgg
     1321 cgtggcaatg aaagggaaat tccgcggaca ggagcaaaag tggttcgcga tgcgattcac
     1381 gggcgacgaa cgtgaaatca atctcaatcc ggaaggccac aagcccgaat tcgacgcctg
     1441 gcgctgggcc gctgcgagcg aagtgctgga cgaaattgtt ggtttcaagc gcgacgctta
     1501 ccgtgcagtc attgaagaat ttcgccctct cctcgcgcct taagcaattg tgctaacacg
     1561 ctgcaataac gacttccgaa ttgccgcagc gggttgcatt gcagcaaggg tctatgctag
     1621 aagcgctcca ggcataagcc gggcttgact ccggacattg gaagcttgac acgaagcacg
     1681 ctataacgcg cccgcgtttc agcggctttt tgactgttgg agagaacgtc ttggctagcg
     1741 gcggccgtga atcgacagtg gcggaacgtt acgcgggagc attgttcaat ctcgcccacg
     1801 agagcaattc cgtcgagagc gtcgagcgtg cgctcgataa gtttctgggt ttgcttcgcg
     1861 acagcgcaga tttgcgccgt ttcgttttga gcccgatgtt ctccgcggtg gagcaggagc
     1921 aggggctgtc cgccatcctg aaatcggctg gcatcacggg acttgcgcgc gactttcttc
     1981 tcgttctcgc gaaaaacagg cgtctgttcg ccaccgagaa cgtcatcaag acttacaaat
     2041 ctctttgcgc caaatcccgt ggcgaggtgg aagcagaggt catttcagcc gcatctctga
     2101 cacaggctca gacccaggat ttgacggata cgctgcggca gaaactcggc aagaccccga
     2161 cgctgacggc taaggtcgat cccaggcttc ttggggggct gatcgtccgc gtcggaagcc
     2221 agatgattga cacttcttta cgtacaaaac tcaagaacct cgaaaagctt atgaaagagg
     2281 ccagctgatg gagcttatgg aaatccgtcc gtccgaaatc tcctcgattt tgcgggagca
     2341 gatcaagaat ttcggacagg aagcagaagt ctccgagatt ggacaggtgc tgtcagtggg
     2401 tgacggcatc gcgcgcgttt acggtctcga taatgtgcaa gccggcgaaa tggtcgagtt
     2461 cccgggcggc gtccgcggga tggctttgaa cctcgaagcc gacaatgtcg gcgtcgttat
     2521 cttcggcgac gaccggaaca tcaaagaagg cgacaccgtc aagcggaccg gcgccatcgt
     2581 ggaggttccc gtcggcaagg ggctgctcgg ccgcgtcgtc gacgcgctcg gcaacccgat
     2641 cgacggcaag ggtccgatcg tcgccgacaa acgcgcccgg gtcgacgtca aggcgcccgg
     2701 catcattccg cgccgctcgg tgaacgagcc gagggcgacc ggcctcaagg cgatcgactc
     2761 gatgatcccg atcggccgcg gccagcgcga gctgatcatc ggcgaccgcc agaccggcaa
     2821 gaccgcgatt gcgctcgaca ccatcctcaa ccagaaggcg ctcaaccaga gcggcgacga
     2881 gagcaagaag ctctattgcg tctacgtcgc gatcggccag aagcgctcga ccgtcgccca
     2941 gttcgtcaag gtgctggagg agcacggcgc gctcgagtac acgatcgtcg tcgccgccac
     3001 cgcctccgac ccagcgccga tgcaatacct cgcgcccttc tccggctgcg cgatgggcga
     3061 attcttccgc gacaacggca tgcacgcggt catcatttac gacgatctct ccaagcaggc
     3121 cgtcgcctat cgccagatgt cgctgttgct gcgccgcccg ccgggccgcg aagcgtatcc
     3181 cggcgacgtg ttctatcttc actcgcgcct gctggaacgc gccgcgaaag ttagcgatcg
     3241 gttgggcggc ggttccctca
//
LOCUS       DATMGX010000872         2143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_114881, whole genome shotgun sequence.
ACCESSION   DATMGX010000872 DATMGX010000000
VERSION     DATMGX010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114881"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1012)
                     /gene="cysK"
                     /locus_tag="VNR65_14320"
     CDS             complement(29..1012)
                     /gene="cysK"
                     /locus_tag="VNR65_14320"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HWJ19893.1"
                     /translation="MAENDIQNTNRAPGRGRIYGSILETIGDTPIVRLDKLAKEKGVK
                     ANLLAKLEFFNPIASVKDRIGVAMIEALEKAGKIEPGKTILIEPTSGNTGIALAFVAA
                     QKGYRLILTMPETMSIERRKLLALLGAELILTPGPLGMKGAIAKAQELLGEYPNAIIP
                     QQFENPANPDIHRRTTAEEIWNDTAHTVDIIISGIGTGGTITGIGQVLKPRKPSLKVI
                     AVEPEESPVLSGGAPGPHKIQGIGAGFVPGVLDRSVIDEVVKVNSATALETARHVARL
                     EGVPVGISSGAAIAAAIEVGLRPSSEGKNIVIIIPSFAERYLSTALFEGLA"
     gene            complement(1563..>2143)
                     /locus_tag="VNR65_14325"
     CDS             complement(1563..>2143)
                     /locus_tag="VNR65_14325"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWJ19894.1"
                     /translation="PELRTNVVLWMASGGHFVHYPVSGGSALNGVLVLDDDYKYAVDG
                     AASDQRPQLLERLSNWAELPRRVIGLTNVWQPWPLYGVEKWDGGHGRIQAIGDAWHAM
                     RPFLASGGVMAIEDGAALASYLDGTTIEEGLKRFREKRGPRVWQVAERSALMGRIYHC
                     PQPFDAVRDLAIKHASPSSLLERNDWLYSAHV"
BASE COUNT          409 a          627 c          654 g          453 t
ORIGIN      
        1 cgggtcaagc ccggccatga cgaacagctt acgcaagccc ctcgaatagc gcggtcgaca
       61 aatagcgctc agcgaacgat ggaatgatga tcacgatgtt cttgccctcg gaggacgggc
      121 gcaggccgac ctcgatggca gcggcaatag ccgcaccgga cgagatgcca accggaacgc
      181 cttcgagccg tgcaacgtgg cgagcggttt caagcgcggt ggcgctgttc acctttacca
      241 cttcgtcgat gacgctgcgg tcgagcacac ccggcacgaa gcccgcgccg atgccctgaa
      301 ttttgtgcgg cccgggcgcc ccacccgaca gcacgggcga ctcctccggc tcgacggcaa
      361 tgaccttcag gcttggcttg cgcggtttca gaacctggcc gatgccggta atcgtgccgc
      421 cggtgccgat gccggagatg atgatgtcga cagtgtgcgc tgtgtcattc cagatctcct
      481 cggctgtggt gcgccgatgg atgtcggggt tggccgggtt ctcgaattgc tgcggaatga
      541 tggcgttcgg atattcgcct agcagttcct gcgctttcgc aatggccccc ttcatgccga
      601 gcggccccgg cgtcagaatg agttccgcgc caagaagcgc gagcagcttg cggcgctcga
      661 tcgacatggt ttccggcatc gtcaggatga ggcgataccc cttctgcgcg gccacgaaag
      721 cgagcgcgat gccggtattg ccggaggtcg gctcgatgag gatggtcttg ccaggctcga
      781 ttttcccggc cttctcaagc gcctcgatca tagcgacgcc gatacgatct tttacactcg
      841 cgatcggatt aaaaaattcg agctttgcca gaaggttggc cttgacgccc ttttccttgg
      901 cgagcttatc aaggcgcacg atgggcgtat cgccgatggt ttcgaggatg gaaccgtaga
      961 tgcgaccccg gcctggagcg cgattggtgt tctgaatgtc gttctcagcc atgacagcct
     1021 ctgggttctt gttgctggct cggctccgct gccgttagat aatcgccacg gctttcatcc
     1081 gatccatcga gagcgggcaa gcgagacatg caaccgcgca tgcctcccga atacttacgc
     1141 tgttgcagag tgagacgcca gggcccgcag catctgcagc aacgaaaaga ataagtccgt
     1201 tgtcagcgtt tcattgcgcg gaaagtccac ttacccctgc ggcccgaatg aagaaatata
     1261 tgctaacgga ggggcttttt tacttttttc tttacgcaga gcctgaaacg gggcgctgac
     1321 aggagaaatt ttacgaaggg tctggttacc ggccatgccc ttcgtccatt gcattttata
     1381 gaccaaagcg ccggctgcgc gctgtagcgg ctacgatccc gtgcgggttg tagtgcacac
     1441 agggtgcgcc gttagaggtc atccgctttt gatagaatca aaagcgggct ctaatttttt
     1501 gtgttgtcgt gcgtctttat cagaaaaccg cttcgcactt ttccggacgc gctctagcct
     1561 ttttaaacgt gcgcgctgta aagccaatcg tttcgctcaa gcaagctgga tggcgaggcg
     1621 tgcttgatcg cgagatcgcg tactgcatcg aaaggctgcg ggcagtggta aatccggccc
     1681 atcagtgccg agcgttccgc cacttgccat acgcgagggc cgcgcttctc ccggaaacgc
     1741 ttcagccctt cttcgatcgt ggtgccatcc aggtaagacg cgagcgcggc gccatcctcg
     1801 atagccatga cgccgcccga ggccaggaag ggacgcatgg cgtgccatgc gtcgccgatg
     1861 gcctggatgc ggccgtggcc tccatcccat ttctcgacgc cgtaaagcgg ccagggctgc
     1921 cagacattgg tcaagccgat gacgcgtcgc ggtagttcgg cccaattgct cagccgttcg
     1981 agaagctgag gcctctggtc ggaggccgcg ccgtcgactg catatttgta gtcgtcgtcg
     2041 aggacgagca cgccgttgag ggcggagccg cctgacacag gatagtggac aaaatggccg
     2101 cccgaagcca tccaaaggac gacgttggtc ctgagttctg gcg
//
LOCUS       DATMGX010000873         5403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_235988, whole genome shotgun sequence.
ACCESSION   DATMGX010000873 DATMGX010000000
VERSION     DATMGX010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235988"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(704..2071)
                     /locus_tag="VNR65_14330"
     CDS             complement(704..2071)
                     /locus_tag="VNR65_14330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HWJ19895.1"
                     /translation="MRVQWTLASGPVGKLRASAMDHPVLVVWLPILLVLAVQAVTRQD
                     SEWQDVYVAAARTLLSGKDLYAPGPYLYPPFSALLAVPFIALPTQAVRLVWYGMNVAA
                     LGVLLYSAWGLAAGPRIRGAGSMPSREWFSLALGTLGCAPYWLNTLAHQQTDLVIAAL
                     LTAGCFVMTRDRIISGAVLIGLAAAFKGPPLLFVGYLAFRRRWLAASVTTVVALGANL
                     LPDLIARPAGETWLGRWVGRILLPTISAPVGTWNADQYNIFNQSLGGTLQRLATSTWS
                     YDTSGLVFSARDMVIEPTTLKAIVYALMLAMLVLSVGAALIGERASPDVPADDRPGRA
                     AIEMTTMIVLVLLMSPMSGLSHFAALAAAMMCLSRMAVVERHRPSLIAFSIALIGALA
                     VNKDLVRATAYDLVLWSGVTTASTIALWLACLLVLICGKASPSTAAFSRTIFFRGPRD
                     LPSQR"
     gene            complement(2369..3313)
                     /locus_tag="VNR65_14335"
     CDS             complement(2369..3313)
                     /locus_tag="VNR65_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19896.1"
                     /translation="MRITAQLINAANGAHLWAERYDRDLTDIFAVQDDVTRHIVDALK
                     IKLTPDEAMRISDIPTTSIEAHDLFLRGREILFGRKWTREIFDTVVAFLRGAIELDPN
                     YAEAYAGLGWAHNFDFHNHLTGSPDALDVAEHFANLAIEKGPKVPFAHWLVATVAVWK
                     RNPERAKRETEIALELSPNYALAYATRGIVEIYSGNPLVAIPFLERAIRLDPAHTQQY
                     IHFLGSAYLVAGKYETAAASFRERIRLAPETDLSRGLLISALGHLGHIEEARRIWAEL
                     KHVNPKYTFAGHLARLPFSNPADVSRIKDGFAKAGLPD"
     gene            3774..3944
                     /locus_tag="VNR65_14340"
     CDS             3774..3944
                     /locus_tag="VNR65_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19897.1"
                     /translation="MTRPSARHPLDLFEGREDYPAQAGRGYGVPGAAKNTGDPARPNA
                     IDLVPRAEIHIP"
     gene            4037..4948
                     /locus_tag="VNR65_14345"
     CDS             4037..4948
                     /locus_tag="VNR65_14345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWJ19898.1"
                     /translation="MSMPLITEIIYCVAVVVWLAVLGIVLFEYVRNPRIFGTTRLLLL
                     VVAIDTCRNLIENVYFGLFFGGQYGFFPAELAESLGNPALVIIPKVLNIVAGGFVLGL
                     LLLRWLPQAVTERGLSDRLVVDLGMLATTDGLTALFNRRHFEELARAEWARFQRYGRP
                     LSLLLLDIDNFKSINDRFGHDAGDLVLKAVAFICKTTKRQTDVAARIGGEEFVLLLPE
                     TDEAAAETTAERLRNAIQVHTRALPGENLEVTVSIGVADASPAMSGFEVLLKRADEAL
                     YEAKRTGRNRVVRALKPALEIFPVAAE"
     gene            complement(4970..>5403)
                     /locus_tag="VNR65_14350"
     CDS             complement(4970..>5403)
                     /locus_tag="VNR65_14350"
                     /inference="COORDINATES: protein motif:HMM:NF018287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HWJ19899.1"
                     /translation="LIDVERRAGYAVLDFWEPMQARPDKSLILDPNEFYILASKEAVQ
                     VPPEYAAEMVPFDPLVGEFRVHYAGFFDPGFGYAGAGGKGARAVLEVRSREVPFVLEH
                     GQIVGRLVYEKMIAKPDKLYGAGIGSNYQAQSLKLSKHFKV"
BASE COUNT         1149 a         1625 c         1639 g          990 t
ORIGIN      
        1 gctgcgtaag cgttgcgggt gtcagtgcct ctacctcaaa gtcccccgcc gccaaatgaa
       61 gcgggcgcag tagaagggtt caatacatcg aggcaattgc cgcgctcttc gcatcgccga
      121 cttttgcaac aaaatctgcc ataagcggac aaactgttgc gggcaaaact ccaactttgt
      181 ccgctgttgt ccgaaagcgg acaaacgggg tgtgtttgga atgtccgctt agtgtggtgc
      241 aggtaaggtg attttatttg gtcaatggtc gggacctttc gggttcccgc gcgttggccc
      301 tgcaccatgg caaaaaccgt cgtaatttcg ctgcttgcag tcttggccgc ttgcccagcg
      361 gtggcgaggg acctctaccc aatcgccgtt cctggggagt gttacgagct agcgcagcgt
      421 gagggcgtac cagtcgtgat ttccaaccgg tacgaagccg caaaagcaaa agttaagttg
      481 gcgcggcttc atgacgcgga ccccaacgtg cacgaatgcc gcgaggcagt taatcgtgcg
      541 cgtcaaacaa taactcaagc ttcccaatag ctctatggcc aaagtcccga ttgtccgctt
      601 tggtccaaaa gcggacaaaa ccgagcgcaa ctggattgtc cgcttagtgc catgagcgtc
      661 gctttgcaca gcagtgaagg gcgaacgcac cgaatgatag cgatcatcgc tgggacggca
      721 aatcgcgcgg ccctctgaaa aaaattgtcc gcgaaaatgc cgccgtcgac ggactggcct
      781 tcccgcagat cagcacgagc aggcatgcca gccagagcgc gatggtactc gccgtggtca
      841 ctccgctcca caacactaga tcgtaggcgg tcgcccggac caaatccttg ttgacggcca
      901 gtgcgccaat cagcgctatg ctgaacgcga tgagcgatgg ccgatgtcgc tccaccacgg
      961 ccatgcgcga caggcacatc atggcggcag cgagcgcggc aaaatgcgac agcccggaca
     1021 tgggcgacat cagcaacacc aggactatca tggtcgtcat ctcgattgcg gcgcgaccgg
     1081 gacggtcatc ggccggcaca tcgggcgacg cccgctcgcc gatcagggcc gcgccaaccg
     1141 acagaaccag catcgccagc atcaatgcgt agacgatcgc cttcagcgtt gtcggctcga
     1201 tgaccatatc tcgcgccgag aacacgagac cgctggtgtc gtaagaccaa gtgctggtcg
     1261 ccaaccgctg aagcgtgccg cctagcgact gattgaaaat gttgtactga tcggcattcc
     1321 acgtcccaac tggagcgctg attgtcggca acagaattcg tccgacccag cggccaagcc
     1381 atgtctcgcc cgccggacgc gcgatcaaat ccggcagaag attggctccg agcgcaacca
     1441 cggtggtcac actggcggcc agccagcggc ggcgaaacgc tagataaccg acaaagagca
     1501 atggaggccc tttgaaggcc gccgcaagtc cgatcagaac cgcaccggag atgatgcgat
     1561 cacgggtcat gacgaagcac ccggcggtca gcagcgccgc gatgacaaga tcggtctgtt
     1621 ggtgggctag ggtattgagc caatagggcg cgcagccaag cgtcccgagt gctaaagaaa
     1681 accactcgcg cgaaggcatg ctgccagcgc cacgaatgcg cggaccggcg gccagacccc
     1741 aggcagaata aagaagcacc cccagcgctg cgacattcat cccgtaccac accaaccgca
     1801 cggcctgggt tggcagcgca atgaacggaa cggcaagcaa cgctgaaaag ggcggataga
     1861 ggtaagggcc tggtgcgtaa aggtccttcc cactgagtaa cgtgcgggcg gcggctacat
     1921 acacatcctg ccattcggaa tcttgcctgg tgacggcttg aacggcgagc acaagcagga
     1981 tcggaagcca gaccacaagg accgggtggt ccattgcgga agcgcgcagc ttgccgacgg
     2041 gtccggatgc caacgtccat tgcactcgca tgcgacagcc ccttgaggac cgtgccggcc
     2101 ctcaataatc gtgtgagtac tctaccttgt gaaattgacg ccgtccggac gaaatcaaga
     2161 aacaggcttc gctaccgcaa catgagatgc gggccgatgt ccgttttggg tcattagcga
     2221 ccgagcaatt caggccccgg acgaccgttt gtccgctatg cccccgatag cgaccgccgt
     2281 cgcggcaatg cagcgaatga cgcaaagtgc cacaagcgaa caaatcttag ggcacaatga
     2341 attgacgcta agtaccagga attcgcgttc agtccggtaa cccggccttg gcaaaaccgt
     2401 ccttgatcct gctgacgtca gcggggttac tgaacggcag tcgcgcaagg tgcccggcga
     2461 atgtgtactt cggatttacg tgcttcaact cagcccatat gcgccgggcc tcttcgatgt
     2521 ggccgaggtg gccgagcgcg gaaattagca agccccgcga taggtcagtt tctggcgcaa
     2581 gcctgatgcg ctcacggaaa gaagccgctg ccgtctcgta cttgccggcg acaaggtagg
     2641 ccgagccgag gaagtgaata tattgctggg tatgagctgg atcgagacgt attgcccgct
     2701 caagaaacgg gatcgctacc aacggattac ccgagtagat ctcgacgatg ccacgcgtag
     2761 cgtaggcaag ggcgtaattt gggctgagct ccaacgcaat ttcagtttct cgcttcgccc
     2821 tttcggggtt ccttttccaa accgccaccg ttgcgacaag ccaatgcgca aaaggtactt
     2881 tagggccctt ttctattgca aggttcgcga aatgctctgc gacatcgagc gcatctggag
     2941 agccggtcaa atgattatgg aagtcgaaat tgtgcgccca gccgagaccg gcgtacgcct
     3001 ccgcgtagtt cggatcgagc tcgatcgcgc ccctcagaaa cgcgacgacc gtatcgaata
     3061 tctcgcgagt ccatttccga ccaaatagga tctcgcgccc gcgtagaaaa aggtcgtgag
     3121 cctcaatgct ggtggttggg atgtcggaaa tgcgcattgc ttcatcgggg gtgagtttga
     3181 tcttgagtgc atcgacaatg tggcgggtga cgtcgtcctg gacggcgaag atgtcagtca
     3241 agtcgcggtc gtagcgctcc gcccataggt gggccccatt tgcagcgttg atcagctgcg
     3301 cggtgatcct cactctgtta ccgacacggc ggattgagcc ctccaaaacc gaccgtacgt
     3361 gatgctgttg cggcggcgac acagcaaaac aaaactcgac cgcaaactta tcctcgctcg
     3421 cttgggcgga tttattatgc gcgtgtccga tccaccgagg gcgctctcat gaggcgtcgt
     3481 gatggcggga tcggatgcgg cgcccgcggg caggagacga acgctcctcc tgctcccggg
     3541 cggcgccggg gttccgcccg ggggcactac gaccccccgc gaggagctcg ctgacggcgg
     3601 cccgtgaggc ttgcccgagg ctcgaaagac gcggccaccc aaaaggggtc gcaacttaga
     3661 gccggaggcg aggcgaaacg gaacgtcaga aagcgcggcc cggcgccgta tggacgtcgc
     3721 gatggcgcgc cgagaggcgc cacgcatcgg aaacggtgcg tgacttacac tgcatgacgc
     3781 gcccatcggc gcgccatccc ctcgatttat tcgaggggcg agaggattac ccggcgcaag
     3841 ccgggagagg ttacggcgta cccggcgccg ccaagaatac gggcgatcct gcgcgaccaa
     3901 acgccatcga cctggtcccg agggccgaaa ttcacattcc gtaaaccaag gcggacagaa
     3961 cagcgccttg aaaacgcccg ttgcgcgcgc ccgcgcgttg ataggtgggg cacaacaaat
     4021 caaatcaggg acagccatga gcatgccgct cataacggag atcatatatt gcgtggcggt
     4081 cgtcgtttgg ctggccgtgc tggggattgt tctcttcgaa tatgtccgca acccgcgcat
     4141 tttcggcacg acgcggctat tgttgctcgt ggtcgcgatc gatacctgcc gcaacctcat
     4201 tgagaatgtt tacttcggac tatttttcgg cggccagtat gggttctttc ccgcggagtt
     4261 ggcggaaagt cttggcaacc ccgcgctcgt cattatcccg aaagtgctca atatcgtcgc
     4321 gggcggcttc gtgctcggcc ttctgctctt gcgctggctg ccccaggcgg tgacggagcg
     4381 cggcctgtcg gaccggctcg tggtcgacct cggaatgctc gcgaccacgg acgggctgac
     4441 cgcgctgttc aatcgccgcc acttcgaaga actggcgcgc gcggaatggg cgcgctttca
     4501 gcgctatggc cgtccgctgt cgctgctgct cctggacatc gacaacttca aatcgatcaa
     4561 cgaccgcttc ggtcatgacg ccggcgactt ggtcctgaag gccgtcgcgt ttatctgtaa
     4621 gacgacgaaa cgccagaccg acgtggcggc gcgtattggc ggcgaagaat ttgtgctgtt
     4681 gctgccggaa accgatgaag cagccgccga gaccaccgcc gagcggttgc gcaatgccat
     4741 tcaagttcat acccgcgccc tgccgggcga gaatctcgag gtgaccgtca gtatcggcgt
     4801 ggccgacgcg agcccggcga tgtcgggttt tgaggttttg ctcaaacgcg ccgacgaagc
     4861 cctgtatgaa gccaagcgga cgggccgcaa tcgcgtggtc agagcgctga aacctgcgct
     4921 cgaaatattt ccggttgcgg ccgaataagg cagccgccaa gcatgaccct cataccttga
     4981 aatgcttgct cagtttcagg ctctgcgcct ggtaattgga gccgatgccg gcgccataga
     5041 gcttgtcggg cttcgcgatc atcttctcgt agaccaaccg cccgacgatc tgcccgtgct
     5101 cgagcacgaa cggcacctcg cgcgagcgca cttcgagcac cgcgcgtgcg cccttgccgc
     5161 cggcgccggc atagccgaag ccgggatcga agaagccggc atagtgcacg cggaattcgc
     5221 cgaccagcgg atcgaacggc accatctcgg cggcgtattc gggcggcacc tgcacggctt
     5281 ccttcgacgc caagatgtag aactcgttgg gatcgaggat caggctcttg tccgggcgcg
     5341 cctgcatcgg ctcccagaaa tcgagcaccg catagccggc gcggcgctcc acgtcgatca
     5401 atc
//
LOCUS       DATMGX010000874         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_50584, whole genome shotgun sequence.
ACCESSION   DATMGX010000874 DATMGX010000000
VERSION     DATMGX010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50584"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VNR65_14355"
     CDS             complement(<1..266)
                     /locus_tag="VNR65_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19900.1"
                     /translation="MDWENRIDFDRLRRERLQKAKDLLKKSSMGSLLCFDMNNVRYLT
                     ATHIGTWAQDKANRFTLLPQNDDPILWDFGSAAKHHALNCPWLGP"
     gene            complement(381..1367)
                     /locus_tag="VNR65_14360"
     CDS             complement(381..1367)
                     /locus_tag="VNR65_14360"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWJ19901.1"
                     /translation="MPERELTFAQAVREALAEEMRRDSRVCIFGEDVAEAGTPFKVLS
                     GLVEEFGAERVVDTPISEAGFTGVGVGAAMTGLRPVVDIMFGDFLTLTMDQMVNQAAK
                     AHYMSGGHWRVPMVMRTTLGATRRSAAQHSQSLHAWFSHVPGLKVVLPSTPYDAKGLL
                     KTAIRDENPVIFFEDKMMYKLKGPVPAEDYTIPLGVADVKRKGDDITLVATSSMVQVA
                     LGAANILEKEGISAEVVDPRTTWPLDEKTIIESAKKTSRVIVLDEGYGRYGVTAELAA
                     VIAEGAFYDLDAPVKRMGAMHVPIPFSPPLEDVTVPTEETVSEAARQMCKKT"
     gene            complement(1360..>2208)
                     /locus_tag="VNR65_14365"
     CDS             complement(1360..>2208)
                     /locus_tag="VNR65_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWJ19902.1"
                     /translation="LYSGEEAVAVGICEALNIDDYITSTHRGHGHCLAKGASPDRMFA
                     ELLGKEAGYCRGKGGSMHIADPATGNLGANAIVGGSVGIATGAAFAAKRLGNGRVAVC
                     FFGEGALGQGSLYEVMNLAQLWKLPVIYVCENNQYNEYTHFSETTAGTILGRARAFGL
                     ESALVDGQDVRAVSEVAMRLVKRARAGGGPAFLQADTYRYSGHHVGDINREYYRSKQE
                     EQLWKTERDPIKLHANWLLAQGHADAAALDQIAAEGRTEMEGAVKFAIAAPYPSIDQV
                     EQDVYA"
BASE COUNT          415 a          722 c          603 g          468 t
ORIGIN      
        1 ggcccgagcc aagggcaatt cagcgcatga tgcttcgccg ccgatccgaa atcccacaga
       61 atcggatcgt cgttttgcgg cagcagcgtg aagcggttgg ccttatcctg cgcccacgtg
      121 ccgatgtgcg tcgcggtcag gtagcggacg ttgttcatat cgaagcagag caacgatccc
      181 atcgacgact tcttgagcag atctttcgcc ttttgcagac gttcacggcg cagccggtcg
      241 aagtcgatcc ggttctccca gtccaccgcc atggttccgt gagtcttgag tcccataaat
      301 cagctcctag ctgctagctt ctagctccta gcttgaaacg agggcgagac gccctcggga
      361 cagccggcgg gacgccggcg ctacgtcttc ttacacattt gtcttgccgc ttctgaaaca
      421 gtttcttccg tcggcaccgt cacatcttcc agcggcggtg aaaacggaat aggcacatgc
      481 atcgctccca tgcgttttac cggcgcgtcg agatcataga acgcaccttc agcgatcacc
      541 gctgctagtt ccgccgttac tccatagcgt ccatagccct cgtccagaac gatcacgcgc
      601 gacgttttct tcgccgactc gataatagtc ttttcgtcga gcggccacgt cgtgcgcgga
      661 tcaaccacct ccgcgctgat gccttccttt tccaggatgt tggccgcacc cagcgcgacc
      721 tgcaccatgc tgctggtcgc caccagcgtg atgtcgtcgc ctttgcgctt tacgtcggcc
      781 acgcccagag gaatcgtgta atcctccgca ggaaccgggc ctttcagctt gtacatcatc
      841 ttgtcttcga aaaatatgac ggggttctcg tcgcggatcg cagtcttcag caagcctttc
      901 gcgtcatacg gtgtcgaagg cagcacaact tttaatccgg gcacgtggct gaaccacgcg
      961 tgcagcgact gcgaatgctg cgctgccgag cgccgggtcg cgcccagcgt ggtacgcatc
     1021 accatgggaa cccgccagtg cccgcccgac atgtagtgcg ctttggcggc ctgattcacc
     1081 atctgatcca tagtcagcgt caggaaatcg ccgaacatga tgtcgaccac cgggcgaagg
     1141 cccgtcatgg ccgcgcccac gcctactccg gtaaatccag cttcggaaat cggtgtatcg
     1201 acaactcgct cggcgccgaa ttcttcgacg agtccggaga gcactttgaa tggcgtcccg
     1261 gcttcggcca cgtcttcgcc gaaaatgcac acgcgcgagt cgcggcgcat ttcttccgcc
     1321 agcgcctccc gcaccgcctg cgcaaatgtg agctcacgct caggcataaa cgtcctgttc
     1381 cacctgatcg atgctgggat acggcgccgc aattgcaaac ttcacagcgc cttccatctc
     1441 agtacgtcct tcggcggcaa tctgatcgag cgctgcggcg tcggcatgcc cttgcgccag
     1501 cagccaattc gcatgcagct tgatgggatc gcgttccgtc ttccagagtt gctcttcctg
     1561 cttcgagcgg tagtattcgc gattgatgtc gcccacgtga tgcccgctgt aacgataagt
     1621 atcggcctgc aggaacgcag gtcctccacc cgcgcgcgcc cgcttcacca gacgcattgc
     1681 gacttcactt accgcgcgca cgtcctgacc gtcgaccaac gcggattcca gaccgaaagc
     1741 ccgggcgcgt ccgagaatgg ttccggccgt cgtctccgaa aaatgcgtgt actcgttgta
     1801 ctgattgttt tcgcagacat agatcacggg caacttccag agctgagcga gattcatgac
     1861 ttcgtagagc gatccttggc caagcgcgcc ttcaccaaag aagcagacgg ccacgcggcc
     1921 gtttcccaga cgctttgcgg caaacgccgc gccagtggcg atacccacac tgccaccaac
     1981 gatcgcattc gcgcccaggt taccggtcgc aggatcggcg atgtgcatcg atccaccctt
     2041 gccccggcag tagccggctt ccttgcccag caattcggca aacatgcggt cgggggaagc
     2101 gcctttggcc aggcagtgtc cgtggccgcg gtgcgtgctg gtgatgtagt catcgatgtt
     2161 gagcgcttcg caaatgccca ccgcgacggc ttcttcgccg ctgtaaag
//
LOCUS       DATMGX010000875         2018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_236225, whole genome shotgun sequence.
ACCESSION   DATMGX010000875 DATMGX010000000
VERSION     DATMGX010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236225"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="VNR65_14370"
     CDS             <1..1430
                     /locus_tag="VNR65_14370"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWJ19903.1"
                     /translation="SQTSSRTGRHVIYYVDPMHPAYRSDKPGIAPDCGMQLEPVFAED
                     AGNAPQVALMAQLPAGAVGIDGATQQLLGIRVAPAERSGSARTLRVVGRVVPEDARVY
                     RINSGMDGFIRETYNDSVGVQVRKDQKLASYYAPEILAPASGFLAANARVPGSAGNDG
                     ARTVPFPGALAQQGRSSLQGYTDRLRNLGMSDAQIKWMAGSGQLPESIDVVAPSDGFI
                     LARNISPGQHFMHETEFYRIADLSRVWVVAEVNEQEAPYLRSGGLAKITLKDEGLQLP
                     ARITDSLPQSEAGGGTVKLRLVVDNPQFLLRPEMLVDVELPVRLPPAVTVPLDALVDS
                     GERARVYVERGEGTFEPREVETGWRFGELVEIRRGLQPGERVVVAATFLVDSESRLKT
                     PLPGPAPARTTERQAVARDHLAAARTVRDPSCGMPVDPARAVASGNILAHHGTTYYFC
                     SSKCKQAFQSDPTVSASRHQGGDDD"
     gene            1423..>2018
                     /locus_tag="VNR65_14375"
     CDS             1423..>2018
                     /locus_tag="VNR65_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWJ19904.1"
                     /translation="MINRIIDFSVEHKLAVLAIFAVACIGGWWAMVTMPLDATPDLSD
                     TQVIIFSHWDRSPDILEDQVTYPIVTAMLGAPKVKTVRGFSDFGYSYVYVVFEDGTDL
                     YWARSRTMEYLSSVLPRLPEGVKTELGPDATALGWIYQYALVDTSGKHSLADLRSYQD
                     WYLGYYLRSVPGVAEVAPIGGYTRQYQVNLDPNRLRAYG"
BASE COUNT          400 a          607 c          624 g          387 t
ORIGIN      
        1 attcgcagac aagtagcagg acgggtcggc acgtaatcta ctacgtggac cccatgcatc
       61 ccgcgtacag atccgacaag cccggcattg cccctgactg cggaatgcag ttggagccgg
      121 tctttgccga agatgcgggg aatgcgccgc aagtcgcgct catggcgcaa ctgcccgccg
      181 gagcagtcgg cattgatggc gcgactcaac aactgctcgg tatccgggtc gctccggcgg
      241 agagaagcgg ctccgctcgc acgctccgcg tagtcggccg tgtggttcca gaagacgcac
      301 gggtgtatcg gatcaactcc ggtatggacg ggttcattcg agagacatat aacgattcgg
      361 ttggagtcca ggtcagaaaa gaccagaagc tggcgagcta ctacgcacca gaaatcttgg
      421 cgccagcttc cgggttcctg gccgcgaatg cgcgcgtgcc gggctccgct ggcaacgacg
      481 gggcacggac tgttccattt cccggcgctt tggcccagca ggggaggagc agtcttcagg
      541 gctataccga ccgcctgcgc aatctcggca tgagcgacgc gcagattaag tggatggccg
      601 ggagcggcca acttcccgag agcattgatg tcgtggcgcc ctcagacgga ttcattctcg
      661 cccggaacat ttcgccagga cagcacttca tgcacgaaac ggaattctac cgcatcgcgg
      721 atctcagccg cgtgtgggtg gtcgccgaag ttaacgaaca ggaggcgccg tatctgcgct
      781 ccggtggcct ggcaaagatc accttgaaag acgagggact ccagttacct gcgcgcatca
      841 ccgacagcct gccgcaatcc gaggccggcg gcgggacggt caaacttcgc ctggtagtgg
      901 acaatcccca gttcctccta cggcccgaaa tgttggttga cgtcgagttg cctgtccgtc
      961 ttcctccagc ggtcacggta ccactggacg cattggtcga ttccggagag agggcgcgcg
     1021 tctatgtcga gcgcggcgaa ggcaccttcg aacctcgcga ggtcgaaaca gggtggcgtt
     1081 ttggcgaact ggtcgagatc cggcgagggc ttcagccagg agagcgcgtc gttgttgcgg
     1141 ccacctttct cgtcgactca gaaagccgcc tgaaaacgcc actgcccgga cctgcgccgg
     1201 cgcgcactac cgagcggcaa gctgtcgcgc gcgatcatct ggcggcggcc agaacagtga
     1261 gagacccaag ttgcggcatg ccggtagatc cagccagggc ggtggcttcc ggcaacattc
     1321 tcgcccatca cggcaccacc tactatttct gttccagcaa gtgcaaacag gctttccaga
     1381 gcgatcctac cgtatcagcc agcagacacc agggaggcga cgatgattaa ccggatcatc
     1441 gacttctctg tagagcacaa gctggccgtg ttggccatct tcgctgtggc atgcatcggc
     1501 ggatggtggg cgatggtcac gatgccgctg gatgcgactc cggacctgag cgacacacaa
     1561 gtcatcatct tttcacactg ggaccgcagt ccggacattc tcgaagacca ggtcacatac
     1621 ccgatcgtca cagccatgct gggcgcgccg aaagtcaaga cggtccgtgg cttctccgat
     1681 tttggctact cgtatgtgta cgtcgtgttt gaggatggaa ccgacctcta ctgggcgcgg
     1741 tcgcgaacca tggagtatct ctccagcgtc cttccgcgtt tgcctgaagg ggtcaagacc
     1801 gagcttggcc cggatgctac cgcgctcggg tggatatatc agtacgcatt ggtagatacg
     1861 tcgggcaagc acagcctcgc cgatctgcgc tcttatcaag actggtattt ggggtattac
     1921 ctccgctcgg tgccaggcgt ggccgaggtt gcgccaatcg gcggctacac gcgccagtac
     1981 caggtgaacc tcgacccgaa tcgtctgcgg gcttatgg
//
LOCUS       DATMGX010000876         3255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_115181, whole genome shotgun sequence.
ACCESSION   DATMGX010000876 DATMGX010000000
VERSION     DATMGX010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115181"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..474
                     /locus_tag="VNR65_14380"
                     /pseudo
     CDS             328..474
                     /locus_tag="VNR65_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            675..1022
                     /gene="tnpB"
                     /locus_tag="VNR65_14385"
     CDS             675..1022
                     /gene="tnpB"
                     /locus_tag="VNR65_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HWJ19905.1"
                     /translation="MIGPPTNTRVWIAAGVTDLRRGFTGLSALVESKLEQKPFSGHVF
                     VFRGRRGDLIKVLWYDGDGLCLFAKRLERGRFVWPQAASGTVSLTRAQLSMLLEGIDW
                     RRPERTWEPQLSV"
     gene            1098..2654
                     /locus_tag="VNR65_14390"
     CDS             1098..2654
                     /locus_tag="VNR65_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWJ19906.1"
                     /translation="MSAVALPDLNALPADALRALILAQHEQLISRDREIEHLKLLLAK
                     LHRMQFGRKSEKLERQIEQLELRLEELESKRSEQEPSASTPAPVTASSTPVTKPTRRA
                     LPDHLPRQTRRHEPKETVCPECQGELRKLGEDVSEMLEYVPASFVVIRHVRTKLSCTK
                     CDCIVQAEAPSRPIARGLAGPGLLAHVLVSKYCDHLPLYRQSEIYARQDVELERSTLA
                     DWVGGSAHLLQPLVEALRHYVMAAGKLHADDTPVPVLAPGQGKTKTGRLWTYVRDDRP
                     AGDTASPAVWFAYSPDRKGEHPAQHLKTFRGTLQADAYAGFNQLYENGRIEQAACWAH
                     VRRKFYDLEQAHASPVAREALMRIGTLYGIEEQIRGKPPNERRAVRQAQSKPLLDSLR
                     QWFEATLSKLSRKSETTAAIRYALVRWDALTRYIEDGHIEIDNNAAERSLRGVALGRK
                     NYLFAGSDAGGERAAAIYSLIGSAKLNGLDPEAYLREVLTRIADHPINRIEELLPWNL
                     VASVPPQASV"
     gene            2612..2917
                     /locus_tag="VNR65_14395"
     CDS             2612..2917
                     /locus_tag="VNR65_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19907.1"
                     /translation="MESRRQRPATSQRVKKKANAKAESFLAALSLPNIAQLIEDGEIT
                     IGMLRPVGCVATASDEDCNYAMLVRRRGESLFQLLTRLDQAIDKALTLDIFTDEING"
     gene            complement(3034..>3255)
                     /locus_tag="VNR65_14400"
     CDS             complement(3034..>3255)
                     /locus_tag="VNR65_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19908.1"
                     /translation="WINPGRVITDLRIQLTPSDQGSTSTNIRYCYTGLSPEGNRELEH
                     YTQQWFEANMRNWETTINHYLRTGQKMSA"
BASE COUNT          660 a          917 c         1041 g          637 t
ORIGIN      
        1 tcaaagggac tttcaccctc aagctgtcga acatgctcgg cacacaagaa aaagggccat
       61 ccagatttct ctgaatggcc tttattaatg ccggcaacta cctactctcc cacacacttt
      121 cgcatgcagc acaatcggcc cagcggagca taccttcggt gttccgtaag cgtccgatta
      181 tgaccgtctg gacatctggc tggtaggaag tcattctgcc gaagtggaca cttacggaat
      241 aggtgtccac ttggagcgga gatggacact tcccggcaag ttacagatgg gatcgcgagt
      301 aagagtgagt tgttgagtgc ggctccgcgg caacggcgca gtatcgcgga gaagcggcgg
      361 atcgtcgaag aaaccctggt ggaaggcgcg tcggtggcgc gagtagcgcg ggggcatggc
      421 atcaatgcca accaggtatt tggctggcgt cggttgtacc gggcaggaag acttggggag
      481 cgcaagccgg ccatgaagtt gctgccggtt cgggtgagcg agagtttgcc ggcgcctctg
      541 ccggtgcgtg ggtccgccga cttctcgaaa ccacagccgg gcatgattca catcgaactg
      601 cggcaggcgc aggtacggat cgaaggcagc gccgatccgg cgttggtgcg cgtgttgttg
      661 gagtgcctac ggggatgatc gggccgccga cgaacacgcg agtttggatt gccgcgggag
      721 tgacggactt gcggcgcggg tttaccgggc tcagtgcgct ggtcgaaagc aaactggagc
      781 agaaaccgtt ctcggggcac gtgtttgtgt ttcgcgggcg gagaggcgat ctgatcaagg
      841 ttctgtggta cgacggcgac gggttgtgtt tgttcgcgaa gcgattggag cgcgggcgct
      901 tcgtttggcc gcaagctgcg agcggcacgg tttcgttgac gcgggcgcag ttatcgatgc
      961 tgctggaggg tatcgactgg cggcggccgg agcggacgtg ggagccgcag ctgtcggtgt
     1021 aacccagaag attctttaca caactttttt tgcgcgcaca aggatgaaaa cgtgcgcgtt
     1081 catgcgatac tccgcgcatg tcagcggtgg cgctccccga tctcaacgct cttcccgcag
     1141 atgcgctgcg agcgctgatc ctggcacaac acgagcaact gatctctcgg gatcgtgaga
     1201 tcgagcatct gaagttgctg ctggccaagc tgcatcggat gcagtttggg cgtaagtcgg
     1261 agaagctgga gcggcagatc gaacagttgg agctgcggct ggaggaactg gaatcgaagc
     1321 gcagcgagca agaacccagc gcgtcaacac cggctcccgt gaccgcctct tcaactcctg
     1381 taacgaaacc cacgcgccgc gcactgcccg atcatcttcc gcgacaaaca cgcagacacg
     1441 agccgaaaga aacggtatgc ccggaatgtc agggcgagtt gcgcaagtta ggcgaggatg
     1501 tctcggagat gctggagtac gtgcccgcca gcttcgtggt gattcgccac gtgcgcacca
     1561 agctgagctg cacgaagtgc gattgcatcg tgcaggccga agcgccgagc cgaccgatcg
     1621 cgcgtggcct ggccgggccg gggctgctgg cgcacgttct ggtgagtaag tactgcgatc
     1681 atcttccgct gtatcggcaa agcgagatct atgcgcgtca ggatgtagaa ctggagcgct
     1741 cgacgctggc ggattgggtg ggcggcagtg cgcaccttct tcagccgttg gtggaagcgc
     1801 tgcgccacta cgtgatggcg gcaggcaagc tgcatgccga tgacacgccg gtgccggtgc
     1861 tggctccggg ccaaggcaag accaagaccg gtcgattgtg gacctacgtt cgcgatgacc
     1921 gtcccgcggg cgacaccgca tctcccgccg tgtggtttgc ctactcaccc gatcgcaaag
     1981 gcgagcatcc cgcgcaacat ctgaaaacgt ttcgtggcac gctgcaggcc gatgcctatg
     2041 cgggattcaa tcagctctac gaaaatgggc gtatcgagca agccgcgtgt tgggcgcatg
     2101 tgcggcgcaa gttctacgat ctggaacaag cgcatgcctc gccggtggcg cgggaagcgc
     2161 tgatgcggat cgggacgctg tatggaatcg aagaacagat tcgcggtaag ccgccgaacg
     2221 aacgccgtgc cgtgcgccag gcgcagtcga aaccgctgct cgactctctg cgacagtggt
     2281 tcgaagcgac tttgtcgaag ctctcgcgga agtcggagac gacggcggcg attcgttatg
     2341 cgttggtacg atgggacgcg ctgacgcgct atatagaaga tggacacatc gagatcgaca
     2401 acaacgccgc cgaacgctct ttgcgcggcg tggccctggg ccgcaagaac tacttgttcg
     2461 cgggatcgga tgcgggcggt gagcgcgcag ccgccatcta cagcctgatt ggttcggcga
     2521 aactcaatgg cctcgatccc gaagcctatc tgcgcgaagt gctcacccgc atcgccgatc
     2581 atccaatcaa ccgcatcgag gaactgcttc catggaatct cgtcgccagc gtcccgccac
     2641 aagccagcgt gtaaagaaga aagccaatgc caaggcagag agtttcctgg ccgccttgtc
     2701 gcttcccaac atcgcgcagt tgatcgaaga cggcgagatc accatcggta tgctgcgccc
     2761 ggtcggttgc gtcgccactg cctccgacga agactgcaat tacgccatgc tggtgcgccg
     2821 ccgtggagaa agtttgttcc agttgctcac ccgcctcgac caagctatcg acaaggcgct
     2881 caccctggac atcttcaccg acgagatcaa tggctgatcc ctagctccaa gtgtccacct
     2941 ccatcctcgt ggacacttaa aaaccaccct tgtcaacacg gtctacttcg gacggttacg
     3001 gtgttcctaa ggatgagaac aatcctggag cagctaggct gacatcttct gaccagtgcg
     3061 caagtagtga ttgatcgtgg tttcccagtt tcgcatgttg gcttcaaacc actgctgcgt
     3121 gtagtgctcg agttcgcgat tgccttccgg cgagagcccc gtgtagcagt accggatgtt
     3181 ggtgctcgtc gacccctgat cggagggagt tagttggata cgcaggtcgg tgatgacccg
     3241 gcccggattg atcca
//
LOCUS       DATMGX010000877         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_420815, whole genome shotgun sequence.
ACCESSION   DATMGX010000877 DATMGX010000000
VERSION     DATMGX010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420815"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2173)
                     /locus_tag="VNR65_14405"
     CDS             complement(<1..2173)
                     /locus_tag="VNR65_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HWJ19909.1"
                     /translation="MTETAMHPGNRFNNVHGLPEPQGLYHPSNEHDACGLGLIANIKG
                     VKSHQIVQDGLKILLHLEHRGAVGADPLAGDGAGILVQIPHEFFAAEAERLGFKLPAP
                     GAYAVGQIFMPQDAALRARCEAITAEVFAEEGIPLLGWRDVPVNNEPLPEIVLATEPF
                     HRQVFVARPSSIPDETAFERALYLARRVIANRARAALGPVEVQNFYIVTLSCRTIVYK
                     GMFLSYQVGEYYLDLHDPRFASALALVHQRFSTNTFPSWRLAHPYRMICHNGEINTLR
                     GNVNWMAAREASVSSPLFGESISKLWPISFEGQSDTACFDNALEFLVQGGYSLAHAMM
                     LLIPEAWANNQLMDEKRHDFYQYHASLMEPWDGPAAIAFTDGRQIGATLDRNGLRPAR
                     YIITSDDRVILASEAGVLTVPEEMIVHKWRLQPGKMLLIDLEKGGIISDDEIKAQLAN
                     AHPYREWLNRGQLRVEALPHVERSQPKTNIALLDRQQTFGYTQESWKFLLSPMIETGQ
                     EAIGSMGTDTPISALSSKPKLLHTYFKQNFAQVTNPPIDPIREELVMSLVSFIGPRPN
                     ILDLEGSSNLKWLEVSQPILTNDELERIRRIAEVPDAPFKTITLDITYAADLGASGME
                     RSLDSLCTQAENAVLEGWNIIIASDRAAGPDRIPIPSLLATSAIHHHLIRKGLRTSVG
                     LVVETGEAHEIHQFAVLAGYGAEAINPYLAFETIEANLGELD"
BASE COUNT          488 a          741 c          788 g          517 t
ORIGIN      
        1 tatcaagctc gccgagattc gcttcgatcg tctcgaaggc gagatacggg ttgatggcct
       61 ccgccccata gcctgccaga acggcgaact gatggatctc gtgcgcttcg cccgtctcga
      121 ccacaaggcc gaccgacgtg cgcagaccct tgcggatcaa gtgatggtgg atcgcgctgg
      181 tggcgagaag gctcgggatc gggatgcggt cgggaccggc cgcgcgatcc gaggcgatga
      241 tgatgttcca gccttccagg acggcgtttt cggcctgcgt acacagcgag tcgagcgagc
      301 gctccatgcc ggatgcgccg aggtcggccg cataggtgat gtcgagcgtg atggttttga
      361 aaggcgcatc cggcacctcc gcgatgcggc ggatgcgctc cagctcgtca ttggtcagga
      421 tgggctgcga tacctcaagc cacttgaggt tcgaagagcc ctccagatcg aggatgttcg
      481 gccgcggacc gataaaggag acaaggctca tgaccagctc ctcgcggatc gggtcgatgg
      541 gcggattcgt cacctgggcg aagttctgct tgaaataggt gtggagcagc ttcggcttgg
      601 atgacagcgc cgagatcggc gtatcggtgc ccatcgagcc aatggcttcc tggcccgtct
      661 cgatcatcgg cgagagcagg aacttccagc tttcctgcgt gtaaccgaag gtttgctgcc
      721 ggtcgagcag cgcaatattg gttttcggct gtgaccgctc gacatgtggc agcgcttcaa
      781 cgcgcagctg cccgcggttc agccactccc gatagggatg agcattggcg agctgcgcct
      841 tgatctcgtc gtcagatata atgccgccct tttcgaggtc gatcagcagc atcttgcccg
      901 gttggagccg ccacttatga acgatcatct cctcgggtac ggtcagcacg ccggcttccg
      961 aagctaggat cacgcggtcg tcggatgtta tgatgtagcg ggctggccga aggccgtttc
     1021 ggtcgagcgt tgccccgatc tgccggccgt ccgtgaaggc gatggccgcg gggccgtccc
     1081 aaggctccat cagcgaggcg tgatactgat aaaagtcatg ccgcttctcg tccatcagct
     1141 ggttgttcgc ccaggcttcc ggaatgagca gcatcatggc gtgggcgagg ctgtagccgc
     1201 cctgaaccag gaattcgagc gcgttatcga agcaagccgt gtcggactgc ccttcgaagg
     1261 agatgggcca cagcttggaa atactctcgc cgaacagcgg cgaggatacg ctcgcctccc
     1321 gcgcagccat ccagttcaca ttgccgcgca acgtgttgat ctcgccattg tgacagatca
     1381 tgcgataggg gtgcgcgagc ctccacgacg ggaaggtgtt cgtcgaaaag cgctgatgaa
     1441 cgagcgcaag cgccgacgca aagcgcggat catgcaggtc gaggtagtat tcgcccacct
     1501 gataggacag gaacattcct ttgtagacga tggtgcggca cgacagggtg acgatataga
     1561 agttctggac ctcaacaggg ccgagcgcgg cgcgcgcccg attcgctatg accctccgcg
     1621 caagataaag agcgcgctcg aaggccgttt catctgggat gcttgaagga cgcgccacga
     1681 agacctgccg gtggaagggc tcggtcgcga gaacgatctc cggcaacggc tcgttgttaa
     1741 ccggcacatc gcgccagccg agcagcggga tgccttcctc cgcaaagacc tcggctgtga
     1801 tcgcctcgca gcgtgcgcgc agcgcggcgt cctgcggcat gaaaatctgg ccaacggcat
     1861 aggcgcccgg cgcaggcagc ttgaaaccga gccgctctgc ttccgcagcg aagaattcgt
     1921 gcggaatctg aacgagaatg cccgccccgt cgccagcaag cggatcggcg ccaaccgcac
     1981 ccctgtgctc caggtgcagc aggatcttca aaccatcctg cacgatctgg tggcttttca
     2041 cgcccttgat attggcgatc aggccgaggc cacaggcgtc gtgctcgttg gaaggatgat
     2101 agagaccttg gggttctggg aggccgtgga cgttattgaa cctgttacct ggatgcatcg
     2161 cggtttcggt catggctgct tatttggagc gaagtctttg aatgtctggc gatcttgatg
     2221 tagttcattc ttgcggcgtc tcttctttgg aggccgccct ttctcatttc acttctcctg
     2281 cgcgccttct aaggtgcggg cacgcaaaat ctcgccccga aacgcatttg tttcgctaaa
     2341 acaaatggcc accgggccga gctcgaccgc gtgggctttt cagcccatcg ctccgaagcc
     2401 tgccagaatt ccatgatgat gacaagcctg ctataatgcc gcgcgaccca cgtgcggctt
     2461 ataatttacg cccggcagtt tgcggatggg ggcagacgca aaccctttca ctcctatata
     2521 agagtgctta tccc
//
LOCUS       DATMGX010000878         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_390719, whole genome shotgun sequence.
ACCESSION   DATMGX010000878 DATMGX010000000
VERSION     DATMGX010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390719"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1479)
                     /gene="ltrA"
                     /locus_tag="VNR65_14410"
     CDS             complement(1..1479)
                     /gene="ltrA"
                     /locus_tag="VNR65_14410"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HWJ19910.1"
                     /translation="MMHGPEESDSVIVAVKPTNKAEQSVAEPVEPRTETKGNADQQST
                     RRAQDRGSVSQALERVRKVAKQGKKERFTSLLHHIDPAMLRTAFYAMKRDAAPGVDGM
                     IWKTYEQDLDRRIEELHARVKSGAYRALPSRRSYIPKEDGSKRPLAVAALEDKIVQRA
                     TAAVLSAIYEEDFLGFSYGFRPKRSQHDALDALIVGISSKKVNYILDADIRSFFTEVS
                     QQWVVRFLEHRIGDTRILRLVQKWLRAGVLEDGVVTIEEKGTGQGSVISPLLANVYLH
                     YVFDLWAERWRRREAKGDMIMVRYADDIVVGFQHESDARRFWDAMRERLREFSLSLHP
                     EKTRLIEFGRFAAQNCKRRGRSKPETFKFLGFVLICDKSRRGDFRIRRKSRRDRMGTK
                     LRGIKEELRQRTNRPIPETGKWLAQVVTGYFAYHAVPTNGLALSAFRYHVKHLWHRQL
                     CRRSQRAKVLWTQMTKLVDEFLPKPLILHPWPSVRFAVKHPR"
     gene            complement(1483..1806)
                     /locus_tag="VNR65_14415"
     CDS             complement(1483..1806)
                     /locus_tag="VNR65_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19911.1"
                     /translation="MQVSYSEGIANHTGPESCVCIREDVSEALTGERIGQPSSRENLI
                     VPGADVFIFTEGNMGSAITRAPAQPGVVGDPEHVRKLFAREPGGLGFGRRLDTAGPRR
                     EDEES"
BASE COUNT          359 a          656 c          597 g          469 t
ORIGIN      
        1 ctaccttggg tgtttgacgg cgaaccgcac gctgggccat ggatgaagga tcaggggctt
       61 cggaagaaac tcgtccacca atttcgtcat ctgcgtccat agcactttgg ctctctggct
      121 gcgccgacat agttgccgat gccagagatg tttgacgtga tatcggaacg cggatagcgc
      181 caggccattg gttggcacgg cgtgataggc gaaatagccg gtgacgactt gagcaagcca
      241 ttttcccgtt tcggggatcg gcctgtttgt tcgttgtcgc agctcctctt tgatcccccg
      301 gagctttgtg cccatgcgat ctcgccgtga cttcctccgg atgcggaagt cgccccgtcg
      361 ggacttatcg caaatgagca cgaagccaag gaacttaaag gtttccggct tggaacgtcc
      421 gcgcctcttg cagttctgcg ccgcaaagcg accgaactca attaggcggg tcttctccgg
      481 atgaagcgac agcgagaact cccgcagcct ttcgcgcatc gcatcccaga agcggcgggc
      541 gtcactctca tgctggaagc cgacgacgat atcgtcggca taccggacca tgatcatgtc
      601 gcccttggcc tcgcgccgtc gccagcgttc ggcccaaaga tcgaagacat agtgaaggta
      661 gacgttcgcg agcagtggcg aaatcaccga gccctgaccc gtcccctttt cctcaatcgt
      721 tacgacccca tcttcgagga cgccggctcg tagccatttc tggacgagcc ggaggatgcg
      781 cgtgtcgccg atccggtgct ccaggaagcg gacgacccat tgctgggaaa cttccgtgaa
      841 gaacgaccgg atgtcggcgt cgagtatgta gttcaccttc ttgctgctga tcccgacaat
      901 gagcgcgtcc agcgcatcgt gctggctgcg tttcggtcgg aacccatacg agaacccgag
      961 gaagtcttcc tcgtagatcg cgctcagcac cgccgccgtg gccctctgga cgatcttgtc
     1021 ttcgagggcc gcaaccgcga gcggacgctt gctgccgtct tccttcggaa tgtagctccg
     1081 acgggatggc agcgcccgat acgctccgct tttgacccgt gcgtgcagtt cctcaatcct
     1141 gcgatcaagg tcctgctcgt aggtcttcca tatcatgccg tccacgccgg gcgcggcgtc
     1201 gcgcttcatc gcgtaaaacg ccgttctcag catggcgggg tcgatatggt gcaggagcga
     1261 tgtgaaccgt tccttcttcc cttgctttgc gactttccgt acgcgttcca gcgcctgtga
     1321 cacgcttccc cggtcctgcg cccggcgcgt gctttgctgg tccgcgttcc ccttggtttc
     1381 cgtccttggc tccaccggct ccgcgacgga ttgctccgct ttgttcgtcg gcttcacggc
     1441 tactataacg gaatcagact cctcgggtcc gtgcatcatc ggctacgact cctcgtcttc
     1501 ccgacgcgga ccggcggtgt ccagccgccg gccaaacccg aggcctcccg gttcccgtgc
     1561 aaagagcttc cgcacatgct cagggtctcc gaccacgccg ggctgggcgg gcgctcgcgt
     1621 tatcgcgctc cccatgttgc cttccgtgaa aatgaacacg tcggcgcccg ggactatcaa
     1681 gttttcgcgg ctcgatggct ggcctatgcg ctcccctgtc aacgcttcgc tgacatcctc
     1741 gcgaatgcaa acgcatgact cggggccagt gtggttcgct ataccttcac tgtaagagac
     1801 ttgcactctc tactccttgc cggtcttccc ggcgcaccgt ttttcttgat ttccgctcgc
     1861 agtgcggaag gcagcgagat gttccatagt tcgcgtacgc gtagaggcga cggttgccct
     1921 gtggctgtcc gaagattagt attgtatgcg atggcaaagc cgagctttga atccgtggat
     1981 gaatatatcg catcgcagcc tgaggctgcg cagcgcgtgc ttgagcgcgt ccgcgtaacc
     2041 attcgcaaga cggttcccag cgccgaagaa acgatttcgt a
//
LOCUS       DATMGX010000879         5478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_432977, whole genome shotgun sequence.
ACCESSION   DATMGX010000879 DATMGX010000000
VERSION     DATMGX010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432977"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VNR65_14420"
     CDS             <1..678
                     /locus_tag="VNR65_14420"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ19912.1"
                     /translation="ECANRIASSHADEPGSTRPLFIGQKTAVINAIRAHLAEFGIVAP
                     VGRHGVEELLNVVADPNDKRVPEIARACFSALGAQLRRIKEQILEFDRLIRAWHRSNE
                     MSMRLEEAPGVGPVLATAVVATVADPKSFRTERNFSAWIGIVPKQHSSGGKNKLGNIS
                     KQGNRYLRGLFVAGALAVIRYAKIHGTKHRPWLTALLARRPTKVAAIALANKIARMVW
                     AMMVKGE"
     gene            complement(1171..2067)
                     /locus_tag="VNR65_14425"
     CDS             complement(1171..2067)
                     /locus_tag="VNR65_14425"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ19913.1"
                     /translation="MDKFGAMETFVRIVEKGSLTAAANALDTSLPTVVRTLASLERHL
                     GVMLLHRTTRRIRLTEEGALYLERCRVILSATHEAEETLISREAELRGNLTVGASVLF
                     GRRYVAPVVNDFLRRHPTVTIDLRFGDRVLNLSEEHVDVAVRIAHLKDSSLVAIPVGH
                     VRRVVCATGQYLRSNGIPKVPDDIRKHCCVRHTGLAPRSEWHFKVGRRNVSIPIICRV
                     TCNEIDSSLSACINGLGLGMFLSYQVAPLRKNGRLKYVLEQFEPEPIPVHVVYAHSNL
                     LSNRMHAFVDECVEKLRRTRFD"
     gene            2239..3822
                     /locus_tag="VNR65_14430"
     CDS             2239..3822
                     /locus_tag="VNR65_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19914.1"
                     /translation="MFRRRLTTIAVIAMAGTFAHPAFAAPAEDLGKVHFETSCKPDAQ
                     RQFDRAMLYQHSFWYRASQKSFEDALKADPECAIAYWGIALSLLYNPHVAPPVKNLAE
                     GAAALEKGNATGAKTQRERDYIDALGAMYADYERVDHRTRVLAYLKAMEQLAQRYPED
                     DEAQIYYALALNVGASPADKTYANQLKGAAILEKIWTRQPEHPGIAHYLIHLYDTPAL
                     AEKGLAAARRYAKVAPAAAHAQHMPSHIFTRLGYWQESIASNTESGRVAKEAGDFHDQ
                     LHSMDYLVYAYLQLGQDGKAKAIIDDMTKVTGFTETFLPGPYALAVSPARYAVERGDW
                     KAAAELQVRPSPLANVQAITYFARALGAARSGNPEAAKIDIAKLAELRDKLRDAKDGY
                     WSEQVDIQRQVATAWVLYAEGKRHEALNAMSAAADVEDKTEKHPVTPGVPKPARELYG
                     VMLLDSGNAKEALTAFEATLKKEPNRLGAYAGAATSAEKAGDKAKAKEYYEKIVAIGD
                     GADDNRTEVADARAYLKKL"
     gene            3856..4233
                     /locus_tag="VNR65_14435"
     CDS             3856..4233
                     /locus_tag="VNR65_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19915.1"
                     /translation="MRPLLLSIALGALVAPAHAQSLEVIGYSGYLGEWELTATVTETA
                     PGRKGYFGPLIMKHVGLCTQDGPEEKSGEIHLEISAASSRLDATLSVAGVECSYSGRL
                     SDSYTGTMTCPDREAVPLKLWVK"
     gene            4381..5361
                     /locus_tag="VNR65_14440"
     CDS             4381..5361
                     /locus_tag="VNR65_14440"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ19916.1"
                     /translation="MRRREFIAALAGLALCPVTTRAQQPSRKIPRIGWLVPSPRAEQD
                     NLEEYRRGMQELGYAEGRTVETKYLYAEGEPGRLDKLAATLVTENVDIIVTFSTPGCL
                     AAKRATTIIPIVFAASSDPLSTGIVTSLGHPGGNITGLSVMATDLSAKRLELLQMVVP
                     SIHNIAVLWDSSNPGMALRVRETKLAAEQLNVAFLDSGASDLNGLEASFTALSKQRPE
                     ALLVTAEPFTRLHRDRILDFTMKNRIPTIYEDGGFVRAGGLISYGPNIPSLFHSAAGY
                     VDKILKGAKPADLPVEQPTKFLLIINVKTAKALNLEIPPTLLARADEVIE"
BASE COUNT         1197 a         1568 c         1635 g         1078 t
ORIGIN      
        1 gaatgcgcca accgtatcgc gtcatcacac gccgacgaac caggtagcac gcgccctctg
       61 ttcatcggtc agaaaaccgc ggtgatcaat gcgatccggg cccatcttgc cgagttcggg
      121 atcgttgcac cggtcgggcg ccacggtgtc gaggagctgc tcaacgtcgt agccgatcca
      181 aacgataagc gagttccgga gatagctcgt gcgtgtttct cggcattggg ggctcaattg
      241 cggaggatca aggagcagat actggagttc gaccggctga tcagggcctg gcaccgatcc
      301 aacgagatga gcatgaggct cgaagaagcc cccggcgtcg gtccggtgct ggctactgct
      361 gtggtcgcta ccgttgctga tccaaagtcc ttccgaacag aacgcaactt ctcggcctgg
      421 atcggtatcg tgccgaagca gcactcaagc ggaggcaaga acaagctcgg caatatcagc
      481 aagcaaggta accgctatct gcgtggcctg ttcgttgccg gcgcgctcgc cgtcatccgt
      541 tacgccaaga tccatggcac caaacatcgg ccctggctca cggccttgct ggcgcggcgg
      601 ccgacaaagg ttgcagctat cgcgctcgcc aacaagattg cgcgaatggt gtgggccatg
      661 atggtcaagg gcgagtaagg aacccgtcgc gcttgcgcgg taaacgagat cgcgccggac
      721 aaccggcgtg atgtgaaagt tgggagggca aacagcacgt aatgcagagc cggtcgatcc
      781 ggcgatcgag acaacccact tgtgccaaag cattcttgaa tgcgagttgt tggttgggac
      841 ctgatccgcg gagggcatta tggccagcgg tcacatgaac cgcgtcaacc ggccggacac
      901 gtggctgcac cgaccgatgc tgcaaaacgt gaagaaagct cttgccaact cggagccgtc
      961 cacacatggc acttttcgga gatggcgctc gtgccccttg atgtccggtc attggggtaa
     1021 aaccgacttc atcaagaaac acggcgacgt ccgggtttga ccccagcgga catcaggccc
     1081 ttccaaaaag tgatgagccg tttgcgaatt gcccggatgg tcttctcttg ggtacacaag
     1141 aacataagcg agccaattcg atgctcttcg tcaatcaaat ctcgttcggc ggagcttttc
     1201 gacgcattca tcaacaaagg catgcattct attcgataga agattcgagt gtgcatagac
     1261 gacatgcacg ggaattggct ccggttcgaa ttgttccagg acgtacttga gtcggccgtt
     1321 ttttcggagc ggcgcgacct ggtaggaaag aaacatgccc agacccaacc cattaatgca
     1381 ggcgctcaac gagctgtcga tctcgttgca cgtgaccctg cagataatcg gaatcgatac
     1441 attccgccgc ccgaccttga aatgccattc gcttcgcggt gccaaacccg tatgtctaac
     1501 gcaacagtgt ttccggatat catccggcac cttcgggatg ccatttgagc gcaggtattg
     1561 cccagttgcg caaacaacgc gacgtacgtg gccgacgggg atagccacga gcgaagagtc
     1621 cttgaggtgc gcgattcgaa ccgcaacatc cacatgctcc tcgctcagat tcaagacgcg
     1681 atcgccgaat cgtagatcaa tggtcactgt tggatggcga cgcaaaaaat cattaacaac
     1741 tggggccaca tagcgtcgac cgaacaagac ggaagcgcct acggtcaggt tcccccgcag
     1801 ctccgcttcg cgcgagatta gagtttcttc agcctcgtgc gtggcagaca gaatgacgcg
     1861 gcagcgttca agatatagcg cgccctcctc agtaaggcgg attcgacgcg tagtccgatg
     1921 aagcagcata acgccgagat gccgttccag cgacgccagg gtccgcacca cggtcggcaa
     1981 ggaggtgtcg agggcgttcg cggccgctgt taggctgccc ttttcaacga tccgcacaaa
     2041 cgtttccatc gcaccgaact tgtccatgac ggcattatta cttcattttt tggagtagtc
     2101 gattgctctc agctgtattt attcagtcag attaagctgc gatagtcgcg ttctcaccgc
     2161 cggcggatgg ctgcgtccaa aatagggtgc gcatttgctg cggctctgca cgcatcatgg
     2221 ctagacagga gtccgacaat gttcaggagg cgtctgacaa caatcgcggt catcgctatg
     2281 gcagggacgt ttgcgcatcc ggcgtttgcg gcgccggcgg aagatctggg caaggtccat
     2341 ttcgagacgt cgtgcaaacc ggacgcccag cggcagttcg accgggcgat gctgtaccag
     2401 cattcgttct ggtaccgggc ttcgcagaag tcgttcgagg acgcgctaaa ggccgatccc
     2461 gaatgcgcca tcgcctattg gggcattgca ctgagcctgc tgtacaatcc gcatgtcgcc
     2521 ccgcctgtta aaaacctcgc ggaaggtgct gccgcgctcg aaaagggcaa cgctacgggc
     2581 gcgaagacgc agcgcgagcg cgactacatc gatgcgctcg gtgcaatgta cgccgactac
     2641 gagagggtcg accatcgcac acgcgtgctc gcgtacctca aggcaatgga acaactggcg
     2701 caacgctacc cggaggatga cgaagcgcaa atctattacg cgctcgccct caatgtcggg
     2761 gcttcacccg ccgacaagac ctatgccaat cagctcaagg gggcagcgat tctggagaaa
     2821 atctggacgc gtcagccgga gcatccggga atcgctcatt atctgatcca tctttacgat
     2881 acgccggcgc tcgccgagaa ggggctcgcg gcggcacgac gctacgccaa ggtggcgccc
     2941 gccgcggcgc acgcgcaaca catgccttca cacattttca cccggctcgg ctactggcag
     3001 gagtcgattg cttccaacac cgaatcgggg cgcgttgcga aggaagctgg agatttccac
     3061 gatcagctgc attcgatgga ctatctagtc tatgcctatc tgcagctcgg gcaggacggg
     3121 aaagctaagg cgatcatcga cgacatgacg aaggtcaccg gcttcaccga gactttcctt
     3181 cccgggccat atgcgctggc tgtttcgccg gcccgttacg cagtggagcg cggcgattgg
     3241 aaggccgcag cggagcttca ggttcgcccc agcccgctgg cgaatgtgca agcgatcacg
     3301 tatttcgcgc gtgccctcgg agcggcccgt tcgggcaatc ccgaggccgc caagatcgac
     3361 atcgccaagc ttgccgagtt gcgggacaag ctgcgtgacg ccaaagatgg ctattggtcg
     3421 gaacaggtcg atatccaacg gcaggtcgcg accgcctggg tgctttacgc cgagggcaag
     3481 cgccatgagg cgctgaacgc gatgagcgcc gctgccgacg tcgaggacaa gaccgagaag
     3541 catccggtga ccccgggcgt gcccaagccg gcccgcgaac tttacggtgt catgctgctt
     3601 gacagcggta atgccaagga agcccttacc gccttcgaag cgacacttaa gaaggagccg
     3661 aaccggctgg gtgcctatgc cggcgcggcg acatcggccg aaaaggcggg agacaaggcc
     3721 aaggccaagg aatattacga aaaaattgtc gcgattggcg atggcgcgga cgataaccgg
     3781 accgaggtcg ccgacgcgcg cgcctatctg aagaaactct gagtggcacg cggtggaagg
     3841 caacgggagc catcagtgag accactcctt ctgtcgatcg cgctgggcgc gcttgtcgcg
     3901 ccggcgcacg cccaatcgct tgaggtcatc ggatactcag gttatctcgg cgagtgggag
     3961 ttgaccgcca ccgttacgga aacggctccg ggccgaaagg gatatttcgg tccgttgatc
     4021 atgaaacacg tcggtctttg cactcaggat ggcccggagg agaagagcgg tgaaattcat
     4081 cttgagatat cggctgcatc atcccgattg gatgctacct tgtcggtggc cggggtcgaa
     4141 tgcagttaca gcggacgatt gtccgattct tataccggca cgatgacctg ccccgatcgg
     4201 gaagcggttc cgctaaaact gtgggttaaa tagtcacgcc cgacacgtgg tgcagcgtcg
     4261 cggtctcgca gcgaagcagc cggagaaatc aggcgcgtcg ggctcggaat tgttgccggc
     4321 catcgttgtt gcaatttggc aacgctgctt tcttcgccga cgttcgcaac aggtggcgcc
     4381 atgagaaggc gcgagttcat tgccgcactg gccggcctgg cattgtgtcc ggtgacgacc
     4441 cgcgcgcagc aaccatcccg gaaaattccg cggatcggct ggctcgttcc cagccctcgg
     4501 gcagagcagg ataatctgga ggagtaccgt cgcggcatgc aggagctcgg ctatgccgag
     4561 ggtcgtacgg tcgagacgaa gtatctttac gccgagggcg agcccggccg tctggataaa
     4621 cttgccgcca cgcttgtgac tgagaacgtc gacatcatcg tcaccttcag tacaccaggc
     4681 tgtcttgctg cgaagcgggc aacaacgatc attccaatcg tcttcgctgc cagtagtgat
     4741 cctctcagta ccggtatcgt tactagtctc ggccaccccg gcggaaatat taccgggcta
     4801 tcggtgatgg cgaccgattt gagtgcgaag cggctagagc tgttacaaat ggtggtgccg
     4861 agcattcaca acatcgcggt gctctgggac tcctccaacc ccggaatggc actgcgcgtg
     4921 cgcgaaacca agctcgcggc cgagcagttg aatgttgctt ttttggattc cggcgcaagt
     4981 gatcttaatg gcctcgaagc aagtttcact gcgctgtcaa aacagcgacc tgaggcgttg
     5041 ctggtaaccg cggagccatt tacgaggctc catcgagacc ggatccttga tttcaccatg
     5101 aagaaccgta ttcccacgat ctatgaagac ggtggtttcg tgcgggctgg cggccttatt
     5161 tcttatggac cgaatatccc gagcctgttc cacagcgcag cgggctatgt ggacaagata
     5221 ctgaaagggg ctaagccggc agatctcccg gtggagcaac cgacgaagtt tctactgatc
     5281 atcaatgtta aaacagcaaa agcgctcaat ctcgaaattc caccgacgct gctcgctcgc
     5341 gcagacgagg tgatcgaatg agcacgataa ctcccgttat tggcactttc gcatgtattg
     5401 gttgggccga cgcggaacga taccaatagg ggtgaacgtg taaaccgctt cgccgcgttg
     5461 attgtaggcg gtccattt
//
LOCUS       DATMGX010000880         3520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_252109, whole genome shotgun sequence.
ACCESSION   DATMGX010000880 DATMGX010000000
VERSION     DATMGX010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252109"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1749
                     /gene="secD"
                     /locus_tag="VNR65_14445"
     CDS             175..1749
                     /gene="secD"
                     /locus_tag="VNR65_14445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWJ19917.1"
                     /translation="MLALPNIIPISTYDSLPGWAQLPRMPLGLDLRGGTHLLYQIDTA
                     QLRKDWLQSIQTESRRALSEEKIAHSGVVIAGNSVRITMRDPDKTQAALTRLKTLAQP
                     LSASLLTGPNNGTGLDLSVQGGENGVITIEPTPAGLQHRVENAITRSIEVIRRRIDPT
                     GTTEATIQAQGGANGKDRILIQVPGLAPEEVKARVGTTAKLTFQLVDGSMSAEEAEAN
                     GVPPDDMLLPDAEHQGRKILLHKEVIVSGDDLVNAYAGYDNRAGGIGERAVDFEFNSK
                     GASSFARVTRENIGKPFAIVLDNKVISAPVIRSEIPGGRGQITGNFSQEEVNRLSLLL
                     RSGALPAALSIIEERSVGPSLGADSIESGKTAAIVGFAAVSALMIAGYGLFGLFSIFA
                     LVVNIAIIMACLSLFHATLTLPGIAGMVLTMGVAVDANVLIYERIREEMKGGRGIVTS
                     IEAGFDRAYGTIIDSHLTALLAALILYAFGSGTVRGFAVTLSFGIVSSLFTAITVTRL
                     MVSTWLRQTRPTVLEI"
     gene            1771..2964
                     /gene="secF"
                     /locus_tag="VNR65_14450"
     CDS             1771..2964
                     /gene="secF"
                     /locus_tag="VNR65_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWJ19918.1"
                     /translation="MLLKPLFRLPQKMHFHFVKYFQLCFALSIVAIVGTVILLFTNGL
                     NFGIDFKGGTLMQVQTPMAVDAPQLRTTLHGLGIGEIQLQNFGKNDELLIRFPEQEGG
                     PEAQKVAADKVLQALPAGTKELRREQVGSTVSKELINSAIWALVLTNLVIFAYVWFRF
                     EWQFALGAVVSLVHDVMVTLGVWSLFQLDFDLTIVAALLTILGYSINDTVVIYDRIRE
                     NLRKYKRMSLRDLLDLSVNETMSRTIMTVGTVLMALIALYFFGGEVIRGFTFAMLFGV
                     LVGTYSSVYIAAPFLVLIGVKRDWSGVEKGKGKVNQPGAKPSRPEPKSTPTQANAGSL
                     KPPGEDGLEIDSIGFSASIPLGEAETPEVPLPPAAPRKPPGQRTSKKGKGQARAGGGR
                     SKRRG"
     gene            2983..3381
                     /locus_tag="VNR65_14455"
     CDS             2983..3381
                     /locus_tag="VNR65_14455"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HWJ19919.1"
                     /translation="MSAPFERLGTRAPLSLTPGGALQVGGVVHRTALLISSAEIFAWN
                     HASSTSSEADILRFLDAQESRRGEFLLLGTGTALAFPSPAFRAEIDRRYMGLEVMDSS
                     AACRTYNVLIAEARKFTAALLPLANIRATF"
BASE COUNT          683 a         1056 c         1049 g          732 t
ORIGIN      
        1 cgagcaagtc caccacctcc gagggagacg aaagcgggaa gcagcccgcc gcgtaactgc
       61 cgcgagccgg taacgcgcca gcgataaaag ggacatttct ccatggtcgg gtttacgttg
      121 cacttttcgc gctggggcgc gttggtaacg cttttcattt cgcttgcgtc gattgtgttg
      181 gcgttgccga acatcattcc gatttcgacc tacgacagct tgccagggtg ggcgcagcta
      241 ccgcgcatgc ccttgggcct ggatcttcgg ggcgggacgc acctgcttta tcagatcgat
      301 accgcgcagc tccgcaagga ttggctacaa tcgattcaga ccgagtcccg gcgggcgctc
      361 agtgaggaga agatcgcgca ttccggggtt gttatcgcag gcaattcggt ccgcatcacc
      421 atgcgagacc cggataagac gcaagccgcg ctgacaaggc tcaagacctt ggctcagcca
      481 ctctcggcat cgcttcttac aggcccgaac aacggaacag gcctggacct ctcggttcag
      541 ggcggcgaaa acggtgtcat caccatcgaa ccgactccag ccgggctgca acaccgggtc
      601 gagaacgcga tcacgcgatc gatcgaggtt atccggcgcc gtatcgatcc tacgggcacg
      661 accgaggcca ccatccaggc tcagggcgga gcgaacggca aggaccgcat tctgatccag
      721 gtgccgggct tggcgcctga agaggtgaag gcgcgcgtcg gcaccacggc gaaactgaca
      781 tttcaactcg tcgatgggtc catgtctgcg gaggaggcgg aggccaatgg cgtcccgccg
      841 gatgacatgc tcttgcccga tgccgagcat cagggccgca agatcctcct tcataaggag
      901 gtgattgtta gcggcgacga tctcgtcaat gcctatgccg gatacgacaa ccgggcgggc
      961 ggcatcggcg agcgggccgt cgatttcgag ttcaacagca agggtgcatc ctccttcgcg
     1021 cgggtgacgc gcgagaatat tggcaagccc ttcgcgatcg ttctcgataa taaggtgatc
     1081 tccgcacctg tcatccggtc ggaaattccg ggcggacggg ggcagattac aggcaatttc
     1141 agccaggaag aggtgaacag gctgtcgctt ctcctgagat cgggcgcgct tcccgctgcc
     1201 ctttcgatca tcgaggagcg ctccgttggc ccgagcctcg gcgcggattc gatcgagtcc
     1261 ggaaaaacgg cagccatcgt cggcttcgcg gctgtctcgg ctctcatgat cgcgggatat
     1321 ggcctctttg gtctcttctc gattttcgcc cttgtcgtca acatcgccat catcatggcg
     1381 tgtctctcgc tgtttcatgc gactcttacc ttgccgggca tcgcgggcat ggtgctcacc
     1441 atgggcgtgg ccgttgacgc caacgtcctt atttacgagc gaatacgcga agaaatgaag
     1501 ggcggccgcg gcatcgtcac atccatcgaa gccgggttcg accgcgccta tggcacgatc
     1561 atcgactcgc atttgacggc ccttctcgcg gccctcatcc tctatgcctt cggttcaggc
     1621 acggtacggg ggttcgccgt cacgctgtct ttcggtatcg taagctccct ttttaccgcc
     1681 atcactgtta cacgcctgat ggtatcgaca tggctgcgac agacgcggcc caccgttcta
     1741 gaaatttagc gccgagcgag cccccaagcc atgctgctga aaccgctatt ccgccttcca
     1801 cagaagatgc acttccattt cgtgaagtac ttccagctgt gcttcgcgct gtcgatcgtc
     1861 gccattgttg gaaccgtcat tcttctgttc acgaacggct tgaatttcgg gatcgacttc
     1921 aagggcggca cgctgatgca ggtgcagacg ccaatggcgg tcgatgcgcc tcagctcagg
     1981 acaacgctcc acggacttgg catcggcgag atccagctcc agaactttgg aaagaacgac
     2041 gagcttttga tccgcttccc tgagcaggag ggcgggccgg aagcgcagaa agtcgctgcc
     2101 gacaaggtgc tccaggcgct gcctgcgggc acgaaggaat tgcgccgcga gcaggtcgga
     2161 agcaccgtat cgaaggaact catcaattcg gcgatctggg cgctcgtgct cacgaatctc
     2221 gtgattttcg cctacgtctg gttccgcttt gaatggcagt tcgcgttggg ggccgtggta
     2281 tcccttgtgc acgacgtcat ggttacactc ggcgtctggt cgctgttcca acttgacttt
     2341 gatctgacca ttgtcgccgc gctgctgacc attctcggct attcaattaa tgacaccgtc
     2401 gtcatctacg acaggattcg agagaacctg cgcaagtaca aacggatgtc gctgcgggat
     2461 ctgctcgacc tttcggtgaa cgagacgatg tcgcggacca tcatgaccgt cgggacggtt
     2521 ctgatggcgc tgattgcgct ttacttcttc ggcggcgagg tcattcgcgg gttcaccttc
     2581 gcgatgctct tcggcgttct tgtcggcacc tattcgtcgg tctacatcgc agcgcctttc
     2641 ctggtcctga tcggcgtcaa gcgtgactgg agcggcgtgg aaaaaggcaa gggcaaggtc
     2701 aatcaacccg gagccaagcc gagccgaccg gagccgaaat ccactcccac ccaggcgaat
     2761 gctggatcgc tgaagccgcc tggagaggat ggtttggaaa ttgacagcat cgggttctcg
     2821 gcgtccatcc cccttggaga agcggagacg cccgaagttc cgctcccgcc ggccgcaccg
     2881 cgcaagcccc ccggccagag gacttcgaag aaaggcaagg gccaggcgag ggcaggtggg
     2941 ggacgctcga aaaggcgcgg ctaggccgcg cccggtcgtg ctgtgagcgc accgttcgaa
     3001 aggcttggca cgagagcgcc tctgtcgctg actcccggcg gcgctctcca ggtgggcggc
     3061 gttgttcata gaacagcgct tctgatttct tcggcagaaa tcttcgcctg gaaccacgcc
     3121 agcagcacga gcagcgaagc agacatcctt cgcttccttg acgctcagga aagccggcga
     3181 ggagagttct tgttgctcgg cactgggacg gcactcgcct ttccatcgcc tgcattcagg
     3241 gccgagatcg accggcgcta tatgggcctc gaggtcatgg acagcagcgc cgcttgccgt
     3301 acctacaacg tccttatcgc agaagcgcgc aagttcacgg cggcgctcct tccccttgcg
     3361 aatatccgcg ccaccttcta gagcatttta gaatcgatca actttttcgc ctctcatcga
     3421 ttacgattaa gggcgggttg atccaaggct tttagttgaa aggttgcttg gagcgcccct
     3481 cttgacctcg gacgacgcaa aacaaagaaa acgtgatgag
//
LOCUS       DATMGX010000881         2493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_338068, whole genome shotgun sequence.
ACCESSION   DATMGX010000881 DATMGX010000000
VERSION     DATMGX010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338068"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VNR65_14460"
     CDS             <1..953
                     /locus_tag="VNR65_14460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ19920.1"
                     /translation="AGLAAAVYAASEGLSVLVLDERAFGGQAGASARIENYLGFPSGI
                     SGMALAGRAFNQALKFGVEIAIPLKVLRLDCSGASGKPMRLELNNGSSVQSRTIVVAS
                     GAHYRRPPIENLSAFEGRGISYWASPVEAKLCEGEEVALVGGGNSAGQAVVFLAPKVK
                     RLHLVVRGAGLEASMSRYLIERIAALPNVELHTGSEVIALDGDEVTGLKAATFRERAT
                     GATRTCPLRHLFLFIGADPNASWLKGCVAFDGKGFVITGANGSDRINSGPRLALPLET
                     NRPGVFAIGDVRAGSTKRVAAAVGEGAAVVAQIHSVLATA"
     gene            1133..1618
                     /locus_tag="VNR65_14465"
     CDS             1133..1618
                     /locus_tag="VNR65_14465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWJ19921.1"
                     /translation="MRRLIFTKPAIRSSKATTLRRAGAGAIATKWLSISGPIPRRRTG
                     LSRAFIERRGRNSPSPQSSTRRDGAGPPIPERDKFLEAIAEDAAATIRDRGCLQFDVL
                     VLANDPNHLFLYEVYESEAAFRAHRGSDYFKKYAATTANMVAKRVTRAMTAISSAQKD
                     R"
BASE COUNT          489 a          691 c          774 g          539 t
ORIGIN      
        1 cggccgggct cgccgccgcg gtctatgccg catcggaagg gctttcggtg cttgtgctcg
       61 acgagcgtgc ttttggggga caggctggag cgtccgcgcg tatcgaaaac tatctcggct
      121 ttccaagcgg catttcaggg atggcgctcg ccgggcgcgc gttcaatcag gccctgaaat
      181 ttggtgtcga gatcgccatt ccgctcaagg tgttgcgact ggattgtagc ggcgcgtccg
      241 gcaaacccat gcgccttgag ctcaacaatg gcagttcggt tcagtcgcgg accatcgtgg
      301 tcgcctcagg cgcccactac cgccggcctc ccatcgaaaa cctttcggct ttcgaaggcc
      361 gtggcatttc ttattgggcg tcgccggtag aggcaaagct atgcgaaggc gaggaggtgg
      421 cgctcgttgg cggcggaaat tcggccggcc aggcggtcgt gttccttgcg ccgaaggtca
      481 agcgcttgca tctcgttgtg cgcggcgcag gcctggaggc atcgatgtca cgttatctga
      541 tcgaacggat tgctgcctta ccaaatgtcg agctgcatac cggttccgag gtcatcgcgc
      601 tcgacggaga cgaggttacg ggccttaaag cggcgacgtt tcgcgagcga gcgacggggg
      661 cgacccgcac atgcccgctt cgtcatctct ttttgttcat cggcgccgac ccgaacgcct
      721 cctggctcaa gggctgtgtt gcgtttgatg gcaaggggtt cgtcatcacg ggcgcgaacg
      781 gctcagatcg gatcaactcc ggtccccgac tggcgttgcc gcttgagacg aaccgccccg
      841 gcgtgttcgc catcggcgat gtgcgggcag gttcgacgaa gcgcgtcgcc gcggctgtgg
      901 gcgaaggggc cgcagtggtc gcacaaatcc acagtgtcct tgcaaccgcc taacgggttg
      961 ccggaaagag atcgccatga gcaaacctgc tcgcacctcg gcagcataaa ggcggtaaaa
     1021 ccaagcgcgc gcggttgcga agaatgcctc aagatcggca gtccgtgggt ccatttacgg
     1081 atctgccgga catgcgggca cgtcggctgt tgcgattcct cgccgaaccg ccatgcgacg
     1141 gctcattttc acgaaaccag ccatccgatc atcgaaggct acgaccctcc ggagggctgg
     1201 ggctggtgct atcgcgacga agtggttatc gatctcggga ccgataccac gccgcaggac
     1261 gggcctatcc cgcgctttta ttgaacggcg cggtcgaaac tcgccctcgc cgcaatcgtc
     1321 gacgcggcgc gacggcgcgg gaccgcctat cccggaacgc gacaagtttc ttgaggccat
     1381 cgcagaagac gccgcagcga cgatcaggga tcgcggctgt cttcagttcg atgtcctcgt
     1441 gctggcgaac gatccgaacc acctcttcct gtacgaggtt tacgaaagcg aagccgcatt
     1501 cagggcgcat cggggatcag attatttcaa gaaatatgca gcaacgaccg ccaatatggt
     1561 cgcaaagcgg gtgacacggg cgatgacagc gatctcgtct gcgcaaaagg accgttaggg
     1621 gctggatgcc gtaagcactc agacgccgaa gagttgcgat ctcctgaaca aacggtgctc
     1681 tcgctttttt gatgggcggt ttactggaaa ggcgtagtaa cctatattta cgaatatttg
     1741 gatttctctt tttccttttg acaaaaatca atattcataa ttttcaacgc gttagtggtg
     1801 cggggtgacc gatgcttgag ttggtatcca cgatagtatg tccggtgtgc gggcatcacg
     1861 aaagtgagaa tatgcacgag ggcgtacgcc aatacttcta taagtgcaac gggtgcggca
     1921 gagtgatgaa gccgataaaa ggagattgct gcgtcttctg ctctcatggc gacgtgcctt
     1981 gtccggagac acagatgtat acgggtcggc gcgagaattt cgtctgtacg caatcgtggt
     2041 tcttgctgtg atatccttgc gcgccagatg ccgcccgaac tattcgtaga aatggcggtc
     2101 gggccgagag cggcagtata cgatccagcc gggaggctgg ctgtttagtc ccggagttga
     2161 cacctcagcg tccccttgct ttatgaaccc cggtggttcc ggccagccaa ggagcgtatc
     2221 gacaaaggtg tctctgccgt cctcgggcgg ttgagcagat tgccaatctg aaaatacgtt
     2281 ctccaaatct ggcgggctgc ggagccatca ttttctcatt ctgagggagc gatggccaag
     2341 cgggaaatca ttgtcaagga cacgcatcgc gcgctttggt acgagaatgg tgtgctggtc
     2401 aaagtccttg cagctggtcg gtataagctt cctcgctcgc gcctcccctg gattggcgag
     2461 caaccaatta tcgaggttgt gcttgtcgac gtg
//
LOCUS       DATMGX010000882         3050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_189379, whole genome shotgun sequence.
ACCESSION   DATMGX010000882 DATMGX010000000
VERSION     DATMGX010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189379"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VNR65_14470"
     CDS             <1..455
                     /locus_tag="VNR65_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19922.1"
                     /translation="PLEQTSFGRRDVCAENTVYFADVSHVPDARRFDMGERDHFISME
                     MASIGMEMMADWGAAAVARRLQMLTERIAEGVRGTGVSLPEPRVRAPHILSLGFEEGI
                     PEDLVAGLASEGVYVAARLGRMRISPHVFNDEADTDRFVAALARRLRG"
     gene            848..2791
                     /locus_tag="VNR65_14475"
     CDS             848..2791
                     /locus_tag="VNR65_14475"
                     /inference="COORDINATES: protein motif:HMM:NF011999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HWJ19923.1"
                     /translation="MYNDSLFNAFARSFEARSETEMSMAEYLESCRGDPMRYANAAER
                     LLAAIGEPQMIDTAKDPRLGRIFLNRTMRAYPAFAGFYGMEETIERIVGFFRHAAQGL
                     EERKQILYLLGPVGGGKSSLAERLKSLMEVHPIYVLKAGDELSPVFESPLSLFDPETL
                     GPMIEEKYGIPRRRLNGLMSPWCYKRLEAFGGDISRFRVAKIQPSRLRQIAIAKTEPG
                     DENNQDISSLVGKVDIRKLESFAQNDPDAYSYSGGLNRANQGILEFVEMFKAPIKMLH
                     PLLTATQEGNYIGTENIGAVPFTGVILAHSNESEWQSFKANKNNEAFIDRICVIKVPY
                     CLRVTEEQKIYEKLISGSELASAPCAPATLETLARFSVMSRLRKHENSTVFAKMRVYD
                     GESLKESDPKARSVQEYKDAAGVDEGMDGISTRFAFKILAATYNHDTSELGADPVHLM
                     YTLEQAIRREQLPDEVEKRYLEFIKAELVPRYAEFIGHEIQKAYLESYSDYGQNLFDR
                     YVDYADAWIEDQDFKDPDTGQLLNRELLNQELTKIEKPAGIANPKDFRNEVVKFSLRS
                     RAQHGGKNPSWTSYEKIREVIEKRIFSQVEDLLPVISFGTKKDGDTEKKHGEFVERMV
                     ARGYTERQVRRLVEWYMRVKQAG"
     gene            2811..>3050
                     /locus_tag="VNR65_14480"
     CDS             2811..>3050
                     /locus_tag="VNR65_14480"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HWJ19924.1"
                     /translation="MHIIDRRLNPGSKSLENRQRFLRRARALVQGAVKKSSQDRDIKD
                     VLEGGEVSIPLDGVNEPRFRREGGTRDMVLPGNKKF"
BASE COUNT          656 a          917 c          912 g          565 t
ORIGIN      
        1 ttcccctcga gcagacctcg ttcggccgcc gagacgtatg cgccgagaac acggtctatt
       61 tcgcggatgt cagccacgtt cccgatgccc ggcgttttga catgggcgag cgcgaccact
      121 ttatttcgat ggagatggcc tcgatcggta tggaaatgat ggccgactgg ggcgcggcgg
      181 ccgttgcgcg acgcttgcag atgctgacgg agcggatcgc ggagggcgtg cgcgggaccg
      241 gtgtcagcct gccggagcct cgcgtacggg cgccgcatat cctgagcctt ggcttcgagg
      301 aagggatccc ggaagatctc gttgccggcc ttgcaagcga aggcgtctat gtcgcggcgc
      361 gtctcggccg catgcgcatc tcaccccacg ttttcaacga cgaggccgat accgaccgct
      421 tcgttgccgc attggccagg cgactgcggg gctaactcgc cggacaacgc gaagcccgcg
      481 tggaaccgtg cgacagcgtc gatgccgcag cctcacttac cttgttccca aaggaatgca
      541 gatcgggcgg ccggggttcc cagttctccg cagcctagtt ttagagctaa tcaaggatgt
      601 tgcagcgcgg taacacctgc agaaatcatt tcaaaatctc gcgacggcgc tccgttcgca
      661 cacttattga gcaaattgcc tgcttataac ccaacggctg cacgcgccat gcattgcgtg
      721 ggtaagtctg tcgcgtgttc aattgaaccc atgttcgggt gctgattcgt gaaggggccg
      781 ggcagcggtg atacggaacc tttgcctcgt gttccagtag tcaactaacc gcatgaggag
      841 aaggatcatg tacaacgatt ctctgttcaa cgctttcgcc cgctcctttg aagctcgaag
      901 cgaaaccgaa atgtcgatgg cggaatatct cgaatcgtgt cgaggtgatc ccatgcgata
      961 tgccaacgcc gctgaacggc tgctggcagc gatcggcgaa ccgcaaatga ttgacacggc
     1021 caaggaccct cgccttggcc gtattttttt gaacaggacc atgcgggcct atccggcctt
     1081 cgccggattc tacggcatgg aagaaaccat cgagcgcatc gtcggattct tccggcacgc
     1141 agcgcaaggt ctggaagagc gcaagcagat cctctatctg ctggggccgg tgggcggcgg
     1201 aaagtcatcg cttgcagagc ggctgaagtc actgatggag gttcatccga tctatgtgct
     1261 gaaggccggc gacgaactca gtccggtctt cgagagtccc ctcagcctgt tcgatccgga
     1321 aacgctgggt ccgatgatcg aggaaaagta cggcattccg cgtcgccgcc tcaatgggct
     1381 gatgagtccc tggtgctaca agcggctcga ggccttcggt ggcgacattt cgcggttccg
     1441 cgtcgccaag atccagcctt cgcgcctgcg ccagattgcg atcgcaaaaa ccgaacctgg
     1501 cgacgagaac aatcaggaca tctcgtcgct ggtcggcaag gtcgatattc gaaagctgga
     1561 gagctttgcc cagaacgatc ccgacgccta cagctattcc ggtggcctga accgcgccaa
     1621 ccagggcatc cttgaattcg tcgagatgtt caaggcgccc atcaagatgc tgcacccgct
     1681 gctgacggcg acgcaggagg gcaactacat cggcaccgaa aatatcgggg cggttccctt
     1741 caccggggtc attctggcgc attccaacga atccgaatgg caaagcttca aggccaacaa
     1801 gaacaacgaa gccttcatcg accgcatctg cgtcatcaag gtgccctact gcctgcgggt
     1861 gaccgaggag cagaagatct acgaaaagct catttccgga tccgaactcg cctccgcgcc
     1921 gtgcgcgccg gcaaccctgg agacgctcgc gcgcttttcg gtgatgtcac gcctgcgcaa
     1981 gcacgagaac tcgaccgtgt ttgcgaaaat gcgggtctat gacggtgaaa gcctcaagga
     2041 atccgatccc aaagcccgca gcgttcagga atacaaggac gccgccggcg tcgacgaagg
     2101 catggacggc atctcgaccc ggttcgcatt caagattctc gccgcaacct acaatcacga
     2161 cacctcggag ctcggcgccg atcccgtcca cctgatgtat acgctcgagc aggcgatccg
     2221 ccgcgagcag ctgcccgatg aagtcgaaaa gcgctacctc gaattcatca aggccgagct
     2281 cgttccgcgc tatgcggaat tcatcggcca cgagatccag aaagcctatc tcgaatccta
     2341 ttcggactac ggtcagaacc tgttcgaccg ctacgtcgat tacgccgatg cgtggatcga
     2401 ggatcaggac ttcaaggacc ccgataccgg ccagttgctc aatcgcgagc ttctgaacca
     2461 ggagctgacc aagatcgaga agcccgccgg catcgccaac ccgaaggact tccgcaacga
     2521 ggtcgtcaag ttctcgctgc ggtcgcgcgc ccagcacggc ggcaagaatc cgtcatggac
     2581 cagctacgag aagatccgcg aagtcatcga gaaacggata ttctcccagg tcgaggacct
     2641 tcttcccgtc atctcgttcg gcaccaagaa ggacggcgat accgaaaaga agcacggcga
     2701 gtttgtcgag cggatggtcg cgcgcggcta tactgaacgt caggtgcgcc ggctcgtcga
     2761 gtggtacatg cgcgtgaaac aggccggcta gggcggatgc agcagtggcc atgcacatca
     2821 tcgacaggcg cctgaatccg ggcagcaaaa gcctggagaa ccgccagcgg ttcttgcgcc
     2881 gcgccagggc gctggttcag ggcgccgtca agaagtcttc gcaggaccgc gacatcaagg
     2941 atgtcctgga aggcggcgag gtcagcatcc cgctcgacgg ggtgaacgaa ccgcgctttc
     3001 gccgcgaagg cgggacgcgc gacatggtgc tgccgggaaa caagaaattc
//
LOCUS       DATMGX010000883         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_338156, whole genome shotgun sequence.
ACCESSION   DATMGX010000883 DATMGX010000000
VERSION     DATMGX010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338156"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VNR65_14485"
     CDS             <1..612
                     /locus_tag="VNR65_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19925.1"
                     /translation="ASAAAAGVDLERLLWVRCSERSSLSLHKDHPPRAALGRLEQVLK
                     VTDLLLQGGGFGMVVLDLGDIPTDSARRVPLTSWFRFRRAVEPTATVLLLIEQEPCAK
                     TCASLVVRLERTAVCARDSVAAPARIGLEGWQIVSHGGSGIPENNVVSHAALLRGMQL
                     RAEVVRSSVPPSAKGARDADPGSWTKRKPAQRASADFELHAAW"
     gene            640..>2208
                     /locus_tag="VNR65_14490"
     CDS             640..>2208
                     /locus_tag="VNR65_14490"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19926.1"
                     /translation="MFACIYIPDFPVEAIVRVEPWLRERAVAVLEGKPPLVRVVALNE
                     KARCLGMEVGMTKLQATVFSAPANGVAHSSEAKAENFMAAQIELSSRRPRSKPKPGPA
                     ILRQRSAEQENSAHAALLDVAHAFTPRVEDTASDTLLLDLAGLERLYGAPATMARDLA
                     SRVNAIALEANIALAANPEAALHAARGFSGTTVLPAGEEAHCLGVLPLQVLLDTFDIS
                     MNNGSASDREREKLRAQMLDTLERWGVRDFRTLALLPEHALASRLGEAGTRLQRLAQG
                     AGMRTLALCEPVTHFEEVMELESPVETLEPLSFVLNRLLEQLCARLEARALAVQELHL
                     RLRLERRVADEETTTAQELAGASSHIVGYGNAVERIVRLPVAMRDTKVFLKLLQLELA
                     AHPPGAPITQVWIAAEPAPPRSAQYGLFLPITPEAEKLEITLARISGIVGERRAGIAR
                     LLDTHRRDGFLMDRFVASAEETKQAAAPTMSDVEEQARLALRVFRPPWQLQVKLAEGR
                     PVALAAVTKPVRQGE"
BASE COUNT          409 a          635 c          718 g          446 t
ORIGIN      
        1 gcttcggcgg cggccgccgg cgtggatctc gagcgactgc tgtgggtgcg ttgtagtgag
       61 cgatcgagtt tgtcattgca taaagaccat cccccgcgcg ctgcgctagg ccgtctcgaa
      121 caggtgttga aggtgacgga cctgctgctg caaggcggtg gcttcggcat ggtggtgctc
      181 gatcttggcg atattcccac cgacagtgcg cggcgcgtgc cgctcacctc gtggtttcgt
      241 tttcgccgcg cggtggaacc tactgcgacc gtgctgttgc tcatcgaaca agagccctgc
      301 gcaaagacct gcgcttccct ggtcgtgcgg ctggaacgca cagcggtatg cgctcgcgat
      361 tcagtggcag cgcccgcgcg cattggactg gaaggctggc aaattgtgtc acacggcggc
      421 tcgggcattc ccgagaacaa tgtcgtgagc catgccgcgt tattgcgcgg catgcaattg
      481 cgcgccgagg tagtgcgctc ctccgtccca ccgagtgcaa aaggggcgcg cgatgcggac
      541 cccggttcgt ggacgaaacg caaaccagcg cagcgagcca gcgccgattt tgaactgcac
      601 gccgcgtggt gattctcaac cttcgacttc gaggcttgga tgtttgcttg tatctacatt
      661 cccgattttc cagtggaggc catcgttcgc gtggagcctt ggttgcgcga acgggcggtg
      721 gctgtgctgg aaggcaaacc tccactggtg cgtgtggtgg cgctcaatga aaaagcccgc
      781 tgcctgggaa tggaggtggg catgaccaag ttgcaggcca ctgttttctc agctcctgca
      841 aatggggtag cccattccag cgaagccaag gcggaaaatt tcatggctgc gcagatcgag
      901 ctttcatccc ggcgcccgag aagcaagccg aagcctggtc ctgcgatctt gcgtcagcgc
      961 tcagccgagc aggagaactc ggcccatgcc gcgctgctcg acgttgccca tgcttttacg
     1021 ccgcgcgtgg aagacaccgc tagcgacacg ttgctgctcg acttggccgg tctagagcgg
     1081 ctctacggtg cgcccgcgac catggcgcgc gatctggcgt cgcgggtaaa cgcgattgcg
     1141 ctggaggcaa acatcgcgct ggccgccaat cctgaagcgg ccttgcatgc cgcgcggggt
     1201 ttcagcggga ccaccgtgct ccccgccgga gaagaggcac actgcctggg agtgctgccg
     1261 ttgcaggtat tgctggatac attcgatatc tcgatgaaca acggttctgc ttccgataga
     1321 gaacgcgaaa agctgcgtgc acagatgctc gacacgctgg aacgatgggg cgtgcgcgat
     1381 ttccgcaccc tggcgctact gcctgagcat gcgctggctt cacggctggg cgaagcgggc
     1441 acgcgtttgc agcgcctggc ccagggagca ggcatgcgca cgctagcgtt atgcgagcca
     1501 gttacccatt ttgaagaggt catggaattg gaatctccgg tcgaaacgct cgagccgctg
     1561 tcgtttgtct tgaaccggct gctggaacaa ctatgtgccc ggctggaagc ccgggccctg
     1621 gctgtacagg aactgcattt gcgtttacgg ctggaacggc gagtcgccga tgaggagaca
     1681 acgactgcgc aggaactggc cggtgcttca tcccacattg tgggatacgg gaacgccgtg
     1741 gaacgcattg tgcgcctgcc ggtggccatg cgcgatacca aggtttttct caagctgctg
     1801 cagttggaat tagcggcaca tccaccgggt gctcccatca cacaagtatg gattgcagcc
     1861 gagccagcgc ctccccgttc ggcacagtat ggactttttt tgcccattac tcccgaagcg
     1921 gagaagttgg agattacgct ggcacggatc agcggcatcg tgggcgagcg gcgagcggga
     1981 atcgcgcgtc tgctggatac ccatcggcgg gatggctttc tcatggatcg ctttgtggct
     2041 tcggcagaag agacgaagca ggcggcagct cctacaatgt cggatgtcga ggaacaggca
     2101 cgattagctt tacgtgtatt tcgcccgcct tggcagctac aggtcaaact ggcagaagga
     2161 cgcccagtgg ctttggcggc cgtgacgaag ccagtccgtc aaggagaa
//
LOCUS       DATMGX010000884         5891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_275088, whole genome shotgun sequence.
ACCESSION   DATMGX010000884 DATMGX010000000
VERSION     DATMGX010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275088"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNR65_14495"
     CDS             <1..210
                     /locus_tag="VNR65_14495"
                     /inference="COORDINATES: protein motif:HMM:NF025862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 C-terminal
                     domain-containing protein"
                     /protein_id="HWJ19927.1"
                     /translation="TGWDARELETLLTFLGKGNWNAEIYTDGPNAAKEPKETVLEKRV
                     VTGATVLKCELAPAGGFAVRFTPAP"
     gene            complement(537..1571)
                     /locus_tag="VNR65_14500"
     CDS             complement(537..1571)
                     /locus_tag="VNR65_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19928.1"
                     /translation="MKNKQMLRFMNLILCVGAASTLATAQGQEAKPPASATSAGKPTF
                     PAVLPGKGLAQHDFFYAGEAKSQDMYLVRNGKVVWEFHDPTGKGEISDAVLLSNGNVL
                     FAHQFGVKLINPEKKVLWRYDAPPGTEIHTAQPIGKDRVLYVQNGEPAKVVVVNVVTG
                     KNEREFVVPVRNPKSVHGHFRHARLTEAGTLLVAHMDMGKIAEYDSTGKELWSHTPGT
                     ATWSAERLKNGNTLITGAKLIREVNPAGATIWEFTPADVPDYLFNSMQIAKRLPNGNT
                     LINTWVNQWDGPVNPATAPVQALEVTPDKKIVWALRAWGNPVDLGPSTTIQLLDVPGI
                     PEAVHFGDLK"
     gene            2027..4216
                     /locus_tag="VNR65_14505"
     CDS             2027..4216
                     /locus_tag="VNR65_14505"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HWJ19929.1"
                     /translation="MKPSRSSLWIALLLSSFTMRALQAADSFQDPTLPIEKRVENLVS
                     LLTLDEKIAMLGQVQPAIPRLNIKAFTNFTEGLHGLGWARGGNLTATQFPQSVGLGET
                     WDPEILKQVGAVEGYEARVYFQKFNGSRVGLAIRTPNVDLTRDPRWGRSEESFGEDPF
                     HVGTMAIGLIKGLQGDDPKYLLAASTMKHFLANSNEDGRTSSSSDFDEWNLREYYLVP
                     FEMGIKGANAQSYMASYNAVNKIPDTVSPFIRNIVEKEWGFDGMVCTDAGSLGNLVRQ
                     FHYYADATAAAAGCVKAGISVFLDPYATQVKDALEKKLIAETDIEHNIKGNIRMRMRL
                     GEFDPPEMVPYNKIAGTEEPWFSDKTRAIVRKATQESIVLLKNTPAPSQGAVVAGTED
                     LLPLDRTKMKSIAVIGPSANDVFIDWYAGNLPYAVTPLEGIRNKVGPRVRVRYAADNY
                     TNSAAANIAAESDVAIVFVGNNPTCNAPFGRGCPTSEGKEAVDRKTIELEQAQTQLVQ
                     RVARANPRTIVVVVSSFPYSMAAVQQSAPAILHMAHSSQEEGNALADVLFGDYNPGGR
                     VSVTWPKSLTDLPPMMDYDIRKGRTYMYSKAEPQYPFGFGLSYTTFEYANLKTSADTV
                     AGTGDITVSVEVKNSGKRAGDEVVQMYVSYPGSAVERPIEQLRGFQRIALQPNETRTV
                     RIPLKGSDLSYWDAEKHSFVVEAGKIEVLVGASSADIKFRKTVSVTP"
     gene            complement(4326..>5891)
                     /locus_tag="VNR65_14510"
     CDS             complement(4326..>5891)
                     /locus_tag="VNR65_14510"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HWJ19930.1"
                     /translation="RPGVKVYDFGQNFSGWPAITVRGRPGATLKMIPGELLDANGLVS
                     QRSSGSPQWFSYILKGSGEETWSPRFSYYGFRYLQMEGAGVDVVSVEGRFVHSSAPRI
                     GEFSCSKDLFNRIHNLIDAAIRSNMQSVLTDCPHREKLGWLEETHLLGSAIMYNYGVQ
                     SLYGKIADDMSEAQTAEGLIPDIAPEYTAFAGGFRDSPEWGSAVVLSPWLAYKHFGDR
                     GILAAHYDSMKRYVEYLGGKSAGGIVAYGLGDWYDIGPKPPGVSQLTNLGVTATAIYY
                     ADLTVLRDIARLLGKSGDAEQFSSQADVTRAAFNAKLYNSGSGVYDRGSQTAYAMPLA
                     LGIVPQERRTEALNKLVEDIRAHGNHTTAGDVGFHFVIQALCEGGRSDVIYDILANPD
                     PPSYAAQVAHGATTLTEAWDANPNSSQNHFMLGHAEEWFYRYLAGIDVDFSRRPEERI
                     VLRPTPVGDIDSAQAKYKTPLGPVSINWQRRQGRFFCDVEVPPGTTAWLLLPGKRGQP
                     VRPGRHHFEVPTR"
BASE COUNT         1193 a         1772 c         1814 g         1112 t
ORIGIN      
        1 accggttggg acgcgcgcga actcgaaacg ctgctaacgt tcctcggaaa aggcaactgg
       61 aacgcggaga tctacactga cggaccgaac gccgccaagg agccgaagga aacggtgttg
      121 gaaaaacgcg tcgtcactgg ggccaccgta ctgaaatgcg agctggcgcc ggccggcggc
      181 ttcgctgtcc ggtttactcc ggccccgtag aatggtgggg cagttccgcg ggcctgctgt
      241 cgcttcacaa cctccggacg gaagcaagcg aaatccgccg cggcgatccg cgccactttg
      301 tggtccgaga ctccgaccca cacgtcggcg gcggactttt cgtggaccag cgggaaatca
      361 ccggcaccgc gagtggtggg aagcgcgatg cggctttggc tcgcagcgta aacgcagcac
      421 tgccgcctgt gagcgatagc aggattgtgc cgaagatgct ccagccattt agagccgcgg
      481 tgatgggcag ttcctctatt tcctgaaccc gatcgcagcc ccaggggggg cgccgatcac
      541 ttcaagtctc cgaagtgaac cgcctcggga atgcccggca catcgagtaa ctgaatcgtg
      601 gtcgagggcc ccaagtccac cgggtttccc catgcgcgca aagcccagac aatcttcttg
      661 tcaggtgtga cttccagcgc ctgaacggga gccgtggcgg gattcaccgg cccatcccac
      721 tgattgaccc aggtattgat cagggtgttc ccattgggca accgtttggc gatttgcatg
      781 gagttgaaaa ggtagtccgg cacgtccgcc ggcgtgaatt cccagatcgt ggccccggca
      841 gggttcacct cgcgaatcag cttcgctccg gtgatcaggg tattgccgtt cttcaaacgc
      901 tccgccgacc aggtcgctgt tcccggtgtg tgggaccaga gttccttacc ggtggagtcg
      961 tactcggcga ttttgcccat gtccatgtgg gccacaagca atgtaccggc ctcggtgagc
     1021 cgcgcatggc ggaaatggcc gtgtacgctt tttggattcc tgacggggac tacaaactcg
     1081 cgctcgttct tgccggtgac cacattgacc accacaacct tggcgggctc gccgttttgt
     1141 acgtagagta cccggtcctt gccgatgggc tgcgccgtat gtatttcggt gcccggcggg
     1201 gcgtcatagc gccagagcac tttcttctcc gggttgatca gcttaacccc gaattggtgg
     1261 gcaaagagca cattgccgtt ggaaagcagc accgcgtcgc taatctcacc cttccctgtg
     1321 gggtcgtgaa actcccagac gaccttgcca ttccgcacca ggtacatatc ctgcgatttg
     1381 gcctccccag cgtagaagaa atcgtgctgt gccaggccct tgccgggtag gacggccggg
     1441 aaggtgggct tccccgccga agttgccgag gccggcggtt tggcttcctg tccctgggcg
     1501 gtagcgagcg tagacgcggc gccgacacac aggatcaggt tcatgaatcg tagcatctgt
     1561 ttgttcttca caggatggac tccttaatgt acatgtcact cttgggcacc cggcagtaga
     1621 tgatcgccct gccgaccgcg cctcgcaatc gcacagaacg tagtagcagt cggcccacgt
     1681 gccggtagta tgatttgcgg gccggattcg tgattccggc gatagacacc cagcctctta
     1741 tgtgtccgga acgagccagg taatacgtta ccagaactca ccgaggcaag acatcccggt
     1801 ctggtctggc agccgggccg ttgaaggtgg atgttcagga gcgtgccgcg gatccattcg
     1861 ggctaaaatg agttcttctg tcaaaaggaa gggcatgcca ggttggctgt caattgcaat
     1921 ctgcggtggg gctcgactgc gctcgcgcgt cagcctcagc gtcaccttca cgcataaggc
     1981 ggcccgttcg gcagagatac gacatgttca cacggagact ccatccatga aacccagccg
     2041 atccagtctg tggattgcac tacttctttc ctccttcacg atgcgggcgc tgcaagccgc
     2101 cgattcattc caagatccca ctctgccgat cgaaaagagg gtggagaacc tcgtctcgct
     2161 gctgaccctg gacgagaaga tcgccatgtt gggccaggtg cagccggcga ttccacggtt
     2221 gaacatcaag gccttcacca acttcaccga gggcctgcac ggcctgggct gggctcgcgg
     2281 aggcaatctc acggccactc agtttccgca atcggtggga ttgggcgaaa cctgggatcc
     2341 cgagatcctc aagcaggtag gcgccgtgga aggatatgaa gccagggtct acttccagaa
     2401 attcaacggt tcgagggtag ggctggctat ccgcacgccc aacgtggatc tgacgcgcga
     2461 tccgcgctgg ggccggtccg aggagtcttt cggcgaggat ccctttcacg tcggaacaat
     2521 ggcgatcggg ctgatcaagg gcctgcaggg agacgatccg aagtacctgc tcgccgcctc
     2581 gaccatgaag cactttctgg ctaacagcaa cgaggacggc cgcaccagtt cttcttccga
     2641 tttcgacgag tggaacctgc gcgaatacta cctggttcct ttcgagatgg gcatcaaggg
     2701 cgccaacgcg cagtcttata tggcctctta caacgcggtc aacaagattc ccgacacggt
     2761 gtctccgttc attcggaaca tcgtggaaaa ggaatggggt ttcgacggga tggtctgcac
     2821 cgacgcggga tcgttgggga acctcgtacg gcagttccac tactacgcgg acgcgaccgc
     2881 ggcggccgcg ggatgcgtca aagccggcat ctcagtgttc ctggacccgt acgcgacgca
     2941 agtgaaggac gcgctggaga agaaactgat cgctgaaacc gacattgagc acaacattaa
     3001 gggcaacatc cggatgcgca tgaggcttgg cgagttcgat ccgcccgaaa tggttcccta
     3061 caacaagatc gccgggaccg aggagccgtg gtttagcgac aagaccagag ccattgtgcg
     3121 caaggccacg caggaatcca tcgtgcttct caagaacacg ccggcgccct ctcagggggc
     3181 cgtcgtcgcg ggaacggagg acctgctgcc tctcgacagg accaagatga aatccatcgc
     3241 cgtgatcggg ccctcggcca acgacgtgtt catcgactgg tacgccggaa accttcccta
     3301 cgccgtgacg ccgctcgaag gcatccgcaa caaggtgggg ccgcgcgtgc gagtgcgata
     3361 cgcggcggat aactacacca acagcgccgc cgcgaatatc gcggcggaat ccgacgtcgc
     3421 gatcgttttt gtgggcaaca atcccacgtg caacgcgccc ttcggccggg ggtgcccgac
     3481 tagcgaaggc aaggaggctg tggaccggaa aaccatcgaa ctggagcagg cgcagaccca
     3541 actggtgcag cgcgtcgcgc gggccaaccc caggaccatc gttgtagtag tctccagttt
     3601 cccctactcg atggccgcgg tacagcagag cgctccagcc atcctccaca tggcgcactc
     3661 cagccaggaa gaaggcaacg cgctggccga cgtgctgttc ggagactaca acccgggcgg
     3721 gcgggtctca gtgacttggc cgaaatcgtt aaccgacctg ccacccatga tggactacga
     3781 catccgcaag ggccgcacgt acatgtactc gaaagctgag ccgcagtatc ccttcgggtt
     3841 cggattgagt tacacgacct tcgaatacgc caacctgaag acatccgctg acaccgtggc
     3901 gggaacgggc gacatcaccg tgagcgtgga agtgaagaac agcggcaagc gcgccgggga
     3961 cgaagtggtg cagatgtacg tgagttatcc gggttctgcg gtggagcggc cgatcgaaca
     4021 acttcgagga tttcagcgaa tcgccctaca gccgaacgaa acgcggacgg tgaggattcc
     4081 tctcaaaggg agcgacctct cctactggga cgcggagaag cacagcttcg tggtagaggc
     4141 gggcaaaatc gaagtgctgg tgggggcgtc ctccgcggat atcaagtttc ggaagacggt
     4201 gagcgtaacg ccgtgaccgg aaagcctcgt ttcggccgcg tccagagcgg ccggacacgc
     4261 ggtcccgact gcgccgatgc gtcagacgca cgcgccgcca aaatttatcc ccgaccggcg
     4321 accgctcagc gcgtcgggac ctcgaagtgg tggcggccag gtcggaccgg ttgccctcgt
     4381 ttccccggca acagaagcca ggctgtcgtt cctggcggca cctcaacgtc gcagaagaac
     4441 ctaccctggc ggcgctgcca attgatggaa accgggccga gcggggtctt gtatttggcc
     4501 tgcgcggaat cgatatcccc gaccggagtc gggcgcagca cgatgcgctc ttccggccgg
     4561 cgggaaaagt ccacgtcgat gcccgccagg tatcgataga accattcttc ggcatggccc
     4621 aacatgaaat ggttctgaga ggagttcgga ttggcgtccc aggcctccgt aagggttgtg
     4681 gctccatgcg ccacctgcgc ggcgtaactt ggcgggtccg ggttcgccag tatgtcgtag
     4741 atgacgtcgg agcgcccacc ctcgcagagc gcttgaatca cgaagtgaaa gcccacatct
     4801 ccggccgtgg tatggttgcc gtgcgcgcgg atgtcctcca ccagcttgtt cagggcctcg
     4861 gtgcggcgtt cttgaggcac aatgccaagg gcgagaggca tggcgtatgc agtctggctt
     4921 ccgcgatcgt aaacgccgct gcctgaattg tagagtttgg cattgaatgc ggcgcgcgta
     4981 acatccgcct ggctgctgaa ctgttcggcg tcgccgctct tcccgaggag ccgcgcgata
     5041 tcccgaagaa ccgtcaaatc cgcatagtaa atcgccgtgg cagtgactcc caagttggtg
     5101 agttgtgaaa cgccaggtgg tttggggccg atgtcgtacc agtcgcccag tccgtacgcg
     5161 acaataccgc ccgccgactt gccgccaagg tactcgacgt accgcttcat gctgtcgtaa
     5221 tgcgcggcca ggattccgcg gtctccgaaa tgcttgtacg ccagccacgg acttaggacc
     5281 accgcgcttc cccattcggg agaatcgcgg aaaccgcctg cgaaggccgt gtattccgga
     5341 gcgatatccg ggatcagtcc ctccgcggtc tgcgcttcgg acatgtcgtc ggcgattttg
     5401 ccgtaaaggc tctggacgcc gtagttgtac atgattgccg agcccagcag atgggtctcc
     5461 tccagccaac ccaacttctc gcgatggggg caatcggtaa gcacgctctg catgttgctg
     5521 cggatggcgg cgtcgatcaa attgtggatg cgattaaaga gatccttcga gcaggagaac
     5581 tcgccgattc gcggagccga ggaatggaca aatcgccctt caacggaaac gacgtccacg
     5641 ccggcgcctt ccatttgcag gtagcgaaat ccgtaatagc tgaaccgcgg actccacgtc
     5701 tcctcgccgg agcctttcaa tatgtaggag aaccactgcg ggctgccgga actgcgctgt
     5761 gaaaccaggc cattcgcgtc cagcaactcg cccggaatca tcttgagcgt cgcgccaggg
     5821 cgtccgcgga cggtaatggc cggccagccg gagaagttct ggccgaaatc gtagaccttc
     5881 acgccgggac g
//
LOCUS       DATMGX010000885         3314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_435885, whole genome shotgun sequence.
ACCESSION   DATMGX010000885 DATMGX010000000
VERSION     DATMGX010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435885"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..2705)
                     /locus_tag="VNR65_14515"
     CDS             complement(144..2705)
                     /locus_tag="VNR65_14515"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWJ19931.1"
                     /translation="MDESPSRSSRSPLDKSSGFVRVRGAREHNLKDISVDIPRDALVV
                     FTGVSGSGKSSLAFGTLYAEAQRRYLESVSPYARRLFHQMAVPQVDSIEGLPPAVALQ
                     QQRGSPTTRSSVGSVTTLSNLLRMLYSRAGDYPRGQPLLYAESFSANTPEGACPECHG
                     IGRIHEVTEKSMVPDDSLTIRQRAIAAWPTAWGGQNLRDILVTLGYDVDRPWRDLPKK
                     DRDWILFTDEQPTVPVYAGYTPAESRRAVRRKEEPSYQGTFTGARKHVLHTFANTQSA
                     LMKRRVTQFMTASICPLCRGKRLRAEALSVQFAGFDIADLSRLPLKRVEELLAPYSNE
                     LSASKRETLHPEKAMVVRRIADELIGRLSVLLDLGLGYLTIERSTPTLSPGELQRLRL
                     ATQVRSNLFGVVYVLDEPSAGLHPADTQALLRAFERLKASGNSLFVVEHELDVVRRAD
                     WIVDVGPGAGEQGGQILYSGPPDGLAQVVGSRTRQYLFGNAGRIEATRRHPKDWLRVR
                     AVTRNNLSRLDVDIPLGVFSCVTGISGSGKSSLISQFLVETLTDKLGQRLSSSESTIE
                     DFEEVVETLGGDVVDGMDHVKRLVVVDQKPIGRTPRSNLATYTGLFDHVRKLFASTKQ
                     ARARRYDAGRFSFNIAKGRCATCEGEGFICVELLFLPSVYAPCPTCKGSRYNDKTLEI
                     KIAGKSVADVLGMTVDEAFRFFAHDVGLQRSLGVIREVGLGYIKLGQSATELSGGEAQ
                     RIKLATELQRIQRGGTLYILDEPTTGLHPSDVEKLVRQLNSLVDGGSSVIVVEHDMMM
                     VAQSDWVIDMGPGAGDEGGLVVAVGPPTAVASVKESRTARYLKRALAEKVRDTQF"
     gene            complement(2765..3040)
                     /locus_tag="VNR65_14520"
     CDS             complement(2765..3040)
                     /locus_tag="VNR65_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19932.1"
                     /translation="MFDTSAQIKLKQPRGTALINMAVASLIIAALYLGREIFVPVALD
                     VLLSFVLAPFVGRLQSLRLQRVVAVLAVVILGFSIIFSLGGPPLAPF"
     gene            complement(3105..>3314)
                     /locus_tag="VNR65_14525"
     CDS             complement(3105..>3314)
                     /locus_tag="VNR65_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19933.1"
                     /translation="GRKPMPKENDLEGQQDQGGKHGGQAGMPKPEQSPPSLKTEIPGL
                     DRKGEVVGDPNTSRSKTAGALKKPL"
BASE COUNT          688 a          984 c          943 g          699 t
ORIGIN      
        1 atcgcaaaag accgggcgaa tgcctcttga gccagatatc cttcagcctt ttgctcggct
       61 tgcctggatt ttagatcggc aacatggccc tgccaacggc gccatcccta atatttaaaa
      121 gatgtctttt gatgtggcac acgttagaat tgggtatcgc gtactttttc agcaagagcg
      181 cgcttcagat accgtgcggt cctgctctcc ttcactgaag ctacagcagt cggaggcccg
      241 acggccacca cgagcccgcc ttcgtctccg gcaccggggc ccatatcgat gacccaatcg
      301 ctttgagcga ccatcatcat gtcatgctcg acaacgatga cggaactgcc gccgtcgacc
      361 aaactgttca actgtcttac aagcttttca acgtcggaag gatgcagacc ggtcgtcggt
      421 tcgtcgagaa tgtaaagggt tcctccgcgc tgtattcgtt gcagctcggt cgcgagcttg
      481 atcctttggg cttcgccgcc cgacagctcg gtcgccgact gtcccaactt gatgtaaccg
      541 agcccgactt ccctgatgac accgaggctg cgttgcaggc cgacgtcgtg ggcgaagaaa
      601 cggaatgctt cgtcgacggt cataccgaga acgtcggcga cggacttgcc ggcgatcttg
      661 atctctagcg tcttgtcgtt gtagcgcgac cccttgcagg tcggacacgg cgcatagacg
      721 ctcggcagga acagcaattc tacgcagatg aaaccctcgc cctcgcacgt cgcgcaccgg
      781 cccttggcga tattgaagga gaatcgtcct gcatcgtaac ggcgggcccg cgcctgcttt
      841 gtcgacgcaa acagctttct gacgtgatcg aacaagccgg tgtaggtcgc gagattcgaa
      901 cgcggcgtcc taccgatcgg cttctgatcg acgacgacga ggcgcttgac gtggtccatt
      961 ccgtcgacga cgtcgccgcc gagtgtttcg acgacttctt caaagtcctc gatcgtactc
     1021 tcggacgaag acaaccgttg ccctagtttg tcggtgaggg tctcgaccag aaactggctg
     1081 atcagcgaag acttgcccga tcccgaaatt ccggtcacgc aactgaaaac accaagcgga
     1141 atatcgacgt cgagccggga aagattgttg cgggtgacgg cgcgtacgcg aagccaatct
     1201 ttcgggtgac ggcgcgtcgc ttcgatacgg ccggcatttc caaacaagta ttggcgcgtt
     1261 cgcgatccta cgacctgcgc tagtccatcc ggcggcccgc tgtagaggat ttggccacct
     1321 tgttctccgg cacccggtcc gacatcgacg atccaatcgg cacgtcggac cacgtccagc
     1381 tcatgttcga caacgaacag cgaattgccc gaggccttca accgttcaaa tgcccgcaag
     1441 agcgcctgcg tgtcggcggg gtgcaggccg gcagaaggtt catccagaac gtacacgaca
     1501 ccaaacagat tggaccgaac ctgcgtcgcc aatcgaagcc tttgcagttc acccggagac
     1561 aaggttggcg tgctgcgctc gatggtaaga tatcctaatc caagatcaag aagcaccgaa
     1621 agccgaccga tcaattcgtc cgcgatcctg cgcacgacca tcgccttttc cggatgaagc
     1681 gtttcgcgtt tggatgcgct caattcgtta gaatatggcg cgagtaactc ctctacgcgc
     1741 tttagaggaa gtcgggacaa atcggcaatg tcgaacccgg caaattgcac ggacaatgcc
     1801 tctgcacgga ggcgctttcc gcgacacagc ggacatatgc ttgcggtcat gaattgagtc
     1861 acgcgtcgct tcatcaacgc gctctgcgta ttcgcgaacg tgtgcagcac atgcttcctc
     1921 gcaccggtaa acgtgccctg gtagctcggc tcctcctttc gtctgaccgc tcgtcgtgat
     1981 tctgccggcg tatatcccgc atacaccgga acggtcggct gctcatccgt aaacaggatc
     2041 cagtcgcggt ccttcttcgg cagatcgcgc cacggtctgt cgacgtcgta tccgagcgtt
     2101 acgagaatat cgcgcaggtt ctggccgccc cacgcggtag gccaggccgc gatcgcacgc
     2161 tgacggatgg ttaggctgtc gtccggaacc atggacttct cggtgacctc atgaatccgt
     2221 ccgataccgt ggcactcggg acaggcgcct tccggagtgt tcgcagaaaa ggattcggcg
     2281 tagaggagcg gctgaccccg aggataatcg ccggcgcgcg aatagagcat ccgtagcagg
     2341 ttcgagagcg ttgttacgct accgaccgac gatcttgtcg taggcgatcc cctttgttgc
     2401 tgaagcgcga ccgcaggagg cagtccttca atcgagtcta cctggggcac tgccatttga
     2461 tggaaaagac gcctcgcata cggtgaaaca gattccaagt agcgtcgctg ggcctcggcg
     2521 tacaatgtgc cgaatgcgag cgaggatttg cccgacccgg atacgccggt gaaaacaacc
     2581 agcgcgtccc gtgggatgtc gaccgatatg tccttcaggt tatgttcgcg ggcgccgcgt
     2641 acgcgcacga agccactcga tttatcgaga ggggaccggg atgatcggct cggggattcg
     2701 tccatgcgac tcgttccggc ggatttattc ggtagaacaa cgtgccacac aaagggaggt
     2761 tcagctaaaa tggagccaaa ggaggacccc cgagactgaa gatgattgaa aaccccagga
     2821 tcacgacggc cagcacagcg acaacgcgct gcaatctcag cgattgcaag cgaccgacga
     2881 acggggcaag cacaaagctt aggagaacgt cgagcgcgac cggtacgaag atctcgcgac
     2941 ccaaatacag cgcggcgatg atcaatgaag cgacagccat attgatcagg gccgtgccgc
     3001 gcggctgttt cagcttgatt tgagcggacg tgtcgaacat cttttagttc cggcttaggc
     3061 ggtcgtaggg gagttcacat cacgcgaatt ctcctgcggg ggcgttacag aggcttcttc
     3121 agcgccccgg ccgtcttgct tcggcttgtg ttgggatcgc ccacgacctc accctttcga
     3181 tccaagcccg ggatctcagt tttcagactt ggtggtgatt gttccggctt cggcattccg
     3241 gcctgaccgc catgcttgcc gccctgatct tgctggcctt ccagatcgtt ttcttttggc
     3301 attggctttc tccc
//
LOCUS       DATMGX010000886         2235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_83285, whole genome shotgun sequence.
ACCESSION   DATMGX010000886 DATMGX010000000
VERSION     DATMGX010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83285"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VNR65_14530"
     CDS             complement(<1..280)
                     /locus_tag="VNR65_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19934.1"
                     /translation="MDFVFMLTRSDQTVTDCLEVFEEIRPLGLTHIGFKDVGVSPEVL
                     AGLASRIKAAGSTTYMEVVSTSPEACLRSARVARDIGIDWLLGGTQVEE"
     gene            complement(494..1948)
                     /locus_tag="VNR65_14535"
     CDS             complement(494..1948)
                     /locus_tag="VNR65_14535"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19935.1"
                     /translation="MSPQTLSETVEGLPNISGSEDLIAEAMARSGPFYLPESGVDFGK
                     IRSAFAIALHMHQPLVPAGGGDLQTAAVISNLQSMMDNPGIGDNHNASVFVWCYKRMG
                     EFIPQLMGEGKQPRVMLDYSGELLYGLRAMGLDDVFDDLKRITIDPAYRGAVEWLGTA
                     WGHAVAPSTPAQDFRLHVRAWQHHFAAIFGFDALARVRGFSPPEMALPNHPDVAYEFV
                     RTLKDCGFRWVMVQEHTVENPDGSLIRCPHVPHRLVARNSRGETKSIIAIIKTRGSDT
                     KLVGQMQPYYEAKGLSRVDLDGHSVPPLVTQIADGENGGVMMNEFPPKFMDATRESSG
                     SDTPAVNVSEYLEYLDAIGVREEHLPQIQPIMQKPIWERFPEPAGPEALEKVIKELRE
                     QDGRFHMEGGSWTNNLSWVRGYESVLGPMEKVSALFAEKTQGVATSDPRYRNALFYLL
                     TSQTSCYRYWGSGIWTDYGRELTRRTNDILTYDF"
BASE COUNT          440 a          739 c          641 g          415 t
ORIGIN      
        1 cctcctcgac ctgagtcccg ccaaggagcc aatcgatgcc gatgtcgcgc gccacccgcg
       61 ccgagcgcag gcaggcttcg ggtgacgtgc tcaccacctc catgtaggtg gtcgaacctg
      121 ctgccttaat ccggctggcg agtccggcaa gcacctcggg gctcacgccg acgtccttga
      181 acccaatgtg ggtcaaccca agcggccgga tctcctcgaa cacttcgaga cagtcggtga
      241 cggtctggtc agaccgagtc agcataaaaa cgaagtccat gaccgtcgac ttcctggtct
      301 caagaaagcg gtatactaac tgatccggcc ccgtgaagtc tctgcgcggc acacttcact
      361 cataactaag agccgcgagg gcgatggctt cacgatcagc aatagccaaa ggccgcgaaa
      421 ccgtcgctcg tttacgtccg cttttccaac ctgtcacatc agcgcgagag cgacagcaag
      481 cgagccgcat caatcagaaa tcgtacgtca gaatgtcatt ggtgcgccgc gtgagttcgc
      541 ggccgtaatc ggtccagatc ccgctgcccc agtagcggta gcaactcgtc tggctggtca
      601 agagatagaa cagcgcattg cggtacctgg gatcggacgt cgcgacgcct tgcgtcttct
      661 ccgcgaacag cgcgctcacc ttctccatcg ggccaagcac gctctcatac ccgcgcaccc
      721 aggatagatt gttcgtccag cttccgcctt ccatgtgaaa gcggccgtcc tgttctcgca
      781 gttccttgat caccttctcg agcgcctcgg ggccagccgg ctctggaaat cgctcccaga
      841 tgggcttttg catgatgggt tggatctgcg ggagatgctc ctcccgaacg ccgatggcat
      901 ccaggtactc cagatattcg ctcacattga ccgccggagt gtccgacccg gacgactccc
      961 gcgtggcatc cataaatttc ggcgggaact cgttcatcat cacgccgccg ttctcgccat
     1021 ccgcaatctg ggtaacgaga ggcggcacgg agtggccgtc gaggtccacc cgcgaaagac
     1081 ccttcgcttc gtaatagggc tgcatctgcc cgaccagctt ggtgtcgctg cccctggtct
     1141 tgatgatcgc gatgatcgat ttcgtctccc cgcgcgaatt ccgcgccaca agccggtgcg
     1201 gcacatgcgg gcagcggatg agcgaaccgt ccgggttctc cacggtatgc tcctggacca
     1261 tcacccagcg aaagccgcaa tccttcagcg tccgtacgaa ctcgtatgca acatcgggat
     1321 ggttcggcag cgccatctcc gggggcgaaa aaccgcgcac gcgggccagc gcgtcgaagc
     1381 cgaaaatcgc cgcgaaatga tgctgccacg ccctcacatg cagccggaaa tcctgcgccg
     1441 gggtggacgg cgccaccgcg tgcccccatg ccgtcccgag ccactccacc gcgccgcgat
     1501 aggccggatc gatcgtaatc cgcttcagat cgtcgaacac gtcgtcgagc cccatcgcgc
     1561 ggaggccata aagaagctcg cccgagtaat ccaacatcac ccgcggctgt tttccttctc
     1621 ccatcaactg tgggatgaac tcccccatcc gcttgtagca ccacacgaat acggacgcgt
     1681 tgtgattgtc gccgataccc gggttgtcca tcatggactg caaattgctg atgaccgcgg
     1741 cggtctggag atcgccgcca cccgccggaa ccagcggctg gtgcatgtgg agcgcgatcg
     1801 cgaacgcgct gcgaattttg ccaaaatcga cgccggactc gggcaggtaa aagggtccgc
     1861 ttcgcgccat cgcctcggcg atgaggtcct ccgagccgct gatgttcggg agtccttcga
     1921 cggtttcgga aagggtttgg gggctcataa ctcggctact ccctcggtca aattcggtgc
     1981 taagcctcga ggcaatttta ccctgggcga ttcaagcgcg catctggcgg agtgaccaag
     2041 ccgggcgcgc cggccacgaa ggatgccgag acccgccccg tccaccgtac ctcgcagtgc
     2101 gccattccgt ccagggctaa ccaggctacg gtggctccgt caacgcaagg gcgatatcgt
     2161 aaggccttgc ccagccagca gcccagaagg ccgattcatc tgctcagggt aggcggccag
     2221 ggttgcaagc ggaaa
//
LOCUS       DATMGX010000887         5400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_31800, whole genome shotgun sequence.
ACCESSION   DATMGX010000887 DATMGX010000000
VERSION     DATMGX010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31800"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VNR65_14540"
     CDS             <1..991
                     /locus_tag="VNR65_14540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWJ19936.1"
                     /translation="LSYRNAEGQIVHNEARPILEDMDQLPFVTPVYKRDLKIEKYFIG
                     YLKHPYLSLYTGRGCKSRCTFCLWPQTVGGHRYRTRSVGHVIDEMRWAKAAFPQVKEL
                     FFDDDTFTDDPARAEAIARELGKLNITWSCNAKANVPRKTLEVLKDNGLRLLLVGYES
                     GNQQILHNIKKGMLVDVARRFTKDCHELGIAIHGTFILGLPGETKETIEETIRFAQEI
                     NPHTLQVSLAAPYPGTHLYKQAVDEGWLREDAIDFVNGNGVQLAPLSYPHLNHTEIFE
                     SVEDFYRRFYLRAPKIASIVSEMVRSPTMMKRRLREGVEFFNFLRDRRNNASC"
     gene            complement(1058..2110)
                     /gene="hpnA"
                     /locus_tag="VNR65_14545"
     CDS             complement(1058..2110)
                     /gene="hpnA"
                     /locus_tag="VNR65_14545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HWJ19937.1"
                     /translation="MNYEGSDRVLITGASGFIGSAIVRTFLNAGFKVRVLVRSSSPRE
                     NFADLPVEIAIGDLKERRSIADALKDVRYLVHAAADYRLWTRKPSELMAANVLGTSTI
                     MEEALKTGVEKIVYTSSVCTLAHSSPSDPADEEHSHDGAEAFNPYKRSKILAENAVRG
                     LIAQAGLPAVIVNPTAPIGPRDIKPTPTGRIIVECAAGRMPAYVDTGLNLAHVDDVAE
                     GHLAALKHGVVGERYILGGQNVRLWDMLAEIAHQTGRRPPRVRLPVPAVFPFAVASEA
                     VAWCTGKEPFATRDSLRMARQCMFFNDAKARKELGYTSRPYEQGIADALAWFYGVGML
                     ASPRGELRRGFRLMAR"
     gene            2493..2750
                     /locus_tag="VNR65_14550"
     CDS             2493..2750
                     /locus_tag="VNR65_14550"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HWJ19938.1"
                     /translation="MDGLEIRRKRAIYRALHRGTKELDLILGRYAKEHVSGMDEARLT
                     LFEHLLSLPDADIDQWIRGHETPDDVAVVVAEVREFYGLGA"
     gene            2802..>5400
                     /locus_tag="VNR65_14555"
     CDS             2802..>5400
                     /locus_tag="VNR65_14555"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWJ19939.1"
                     /translation="MASETETLGKKGAAQAATPSALDKLAGGEGTIVAAGVPEGYDAF
                     LLAAIARRLPADTAFQQAVLHIARDDQRLAAIRSQLEFFAPNAEVMAFPAWDCVPYDR
                     VSPDSDIEARRIATLARLAHAKGGKPAIIVLTTVNAVLQRVPPLDALKKAAARLTAGG
                     RVSMDQVIQRLDASGFHRTGTVMEPGEYAVRGGILDLFAPGHARPVRLDFFGDTLESI
                     RQFDPVTQRTQAAREQVTLLPISEIPSGEAAIKHFRQRYVELFGPAKGDDALYEAVSA
                     GSRYPGMEHWLPLFYERMDTLFDYLPNAVITLDHLGDDATARRLETIADHYEARKEGL
                     EQRSFGAPPYKPLPPDALYLTAMEWQGHLEARKLRLFTPFPQPSPKGLQIDFRGKQGR
                     SFAIERTESSEKLIPAVIDHIRTLQGLGKRSIVACWTNGARERLGNMISAHENIRIEK
                     VANIAEARKADKSAVSFALLGLESGFETPDLAIIAEQDILGDRLIRRRKAKKASDVLV
                     EASSLTPGDLMVHADHGIGRFIGLKTIAAAGAPHDCVELEYLGGDKLFLPIENMELLT
                     RYGSDETSANLDKLGGASWQGRKARMKKRIREMAEELIKIAAARETRPAPVFTVPDGA
                     YNEFVTRFPYEETDDQFASIDAVTEDLAKGKPMDRLICGDVGFGKTEVALRAAFLAVM
                     EGKQVAVVVPTTLLARQHFQTFTDRFRGFPVKVAQASRLVASKELAQVREGLKNGQID
                     IVVGTHALLAKSIGFKDLGLLIIDEEQHFGVGHKERLKSLREEVHVLTLTATPIPRTL
                     QLALTGVREMSVIATPPVDRLTVRTFITPFDPVTLREALLRERYRGGQSFYVVPRISD
                     LDEVATFLRE"
BASE COUNT         1093 a         1622 c         1646 g         1039 t
ORIGIN      
        1 cctatcttac cgcaacgccg aagggcagat cgttcataac gaggctcgcc ccatcctcga
       61 agacatggat caactgccgt ttgtgacgcc ggtctataag cgcgatctga agattgagaa
      121 gtatttcatc ggctatctga agcaccccta cctttcgctc tacaccgggc ggggatgcaa
      181 atcacgctgc accttctgcc tgtggccgca gaccgttggc gggcaccgct acaggacgcg
      241 aagcgtcggc cacgtcatcg acgagatgcg ctgggccaag gccgcattcc cgcaagtcaa
      301 agagctcttc ttcgacgatg acacgttcac cgatgatccg gcccgcgccg aggccatcgc
      361 gcgcgagttg ggcaagctca acatcacctg gtcgtgcaac gccaaggcca acgttcctcg
      421 gaaaacgctt gaagttctca aggacaacgg gttgcggctt ctgctcgtcg gatatgaaag
      481 cggcaatcag caaatcctgc acaacatcaa gaagggcatg ctcgtcgacg tcgccaggcg
      541 cttcaccaag gactgccacg aactcggcat cgcgatccac ggcacgttca tcctcgggct
      601 tcctggagag accaaggaga cgatagagga aacgatccgc ttcgcgcagg agatcaatcc
      661 gcacacgctc caggtttcac tcgctgcgcc atatcccggc acgcatctct acaagcaggc
      721 ggtggacgag ggctggctgc gggaagacgc catagatttt gtgaacggga atggcgtgca
      781 actcgcgccg ctaagctacc cgcacctaaa ccacaccgag attttcgagt ccgtcgagga
      841 cttctacagg cggttctatc tgcgggcgcc aaaaatcgcg tcgatcgtgt ccgagatggt
      901 gcgcagcccc accatgatga agcggcgtct gcgcgaaggc gtggagttct tcaacttcct
      961 tcgcgaccgg cgcaataatg ccagctgtta gatcaatctg cctataatcg gaatgggtca
     1021 aagagaacat ccctcgtcca aatgggcgcg ggatgttcta gcgagccatc agcctgaacc
     1081 cccgtctcaa ctcgccgcgg gggctcgcca gcatacccac cccgtagaac caggcgagcg
     1141 cgtccgcaat gccttgctca taaggcctgg acgtataacc caattccttg cgggccttgg
     1201 catcgttgaa gaacatgcac tggcgcgcca tccggaggct atcgcgggtg gcgaagggct
     1261 ccttgcctgt gcaccaggca acagcttcgc tcgccacggc gaatgggaag actgccggga
     1321 ccggaagcct cacgcggggc ggccgccggc ctgtctggtg cgcgatttcc gccagcatgt
     1381 cccacaggcg gacgttctga ccgccgagaa tataccgctc gccgacgacg ccatgcttca
     1441 atgccgcgag atggccctcg gccacatcgt cgacgtgagc aaggttcagc cccgtatcca
     1501 cgtaagcagg catccggccg gcggcgcatt caacgatgat gcgcccggtc ggcgtcggtt
     1561 tgatgtcgcg cgggccaatc ggggccgtgg gatttacgat gacagcggga agtccggcct
     1621 gggcgatgag ccctcgcacg gcattttcgg ccaggatctt actgcgctta tagggattga
     1681 aggcttccgc gccgtcatgg gaatgttcct catcagcggg atccgacggc gaagagtggg
     1741 cgagcgtgca gacgctgctc gtgtagacga tcttttcaac tccggtcttt aacgcctcct
     1801 ccatgatggt gctcgttccg agcacattgg ccgccatcag ctcggagggc tttctggtcc
     1861 aaaggcggta atccgcggcg gcatggacga gatagcggac gtccttcagt gcgtcggcga
     1921 tcgagcgtct ttccttcagg tcgccgattg cgatttcaac cggtaagtct gcgaagttct
     1981 cgcgcggact ggaggagcga acgagaaccc ggactttgaa acctgcattc aagaaagtgc
     2041 ggacgatggc cgacccgata aagccgcttg cgcccgtgat aaggacacga tcgcttccct
     2101 cgtaattcac gatgatttcc tcccagttca gcgccctacg gcttgcggca cggctcaggt
     2161 tcaacctcta tcaaattgag gcataatcga aaagaggtgt gctgttcctc acgtttcccg
     2221 cgttatttcc aagcagtcta attcaactgt ggccagagcg ggacgctcag tgaagtgctt
     2281 ttatgacggg atgtaagaac gcacacaaat ttttcgggcg atgcaaggcg gaaacgctcg
     2341 cggttgcaag ccgatgagct tatgcgatgc catgcgcgtt tgtgttggca agatttaagg
     2401 aacagcggtc acgaatgaat tgcgcgagcg cagaaattgc gggaaagatg caatcgcgcg
     2461 cctcggggcg tgtgattaat agggcttttt tcatggacgg gcttgaaatc agacgcaagc
     2521 gcgcaattta ccgcgccctt catcgcggca cgaaagaact ggatctgatc ttgggccgtt
     2581 atgcaaaaga gcatgtctca gggatggatg aggcgcggct gacactcttc gaacacctcc
     2641 tgtctttgcc cgatgccgat atcgatcaat ggattcgcgg gcacgaaaca cccgacgacg
     2701 tggctgtcgt cgtggcggaa gttcgcgagt tttatgggct cggggcttag cttgcgctga
     2761 tgcgtgggaa tttgagtgtc gtattcacag cgtagatcgt aatggcgagc gaaacagaaa
     2821 ccctcggcaa aaaaggcgcg gcccaggcgg cgacgccgtc cgcactcgat aaactcgctg
     2881 gcggcgaggg caccatcgtg gctgcgggtg tgcccgaggg ttatgacgcc ttcctcttgg
     2941 cggccatcgc gcgcaggctt cctgccgata ccgcgtttca gcaagcggtt cttcatatcg
     3001 cgcgagatga tcaacggctt gcggcgatcc gaagccagct cgaattcttt gcgccgaatg
     3061 ccgaggtgat ggcctttccg gcttgggact gcgttcccta tgaccgcgtt tcgcccgata
     3121 gcgacatcga agcgcgccgc atcgccacct tggcgcggct tgctcatgca aagggcggca
     3181 agcctgcgat tatcgtgctg acaacggtca acgccgtgct ccagcgcgtg cctccgcttg
     3241 acgcgctcaa aaaggcagcg gcgcggctta cggctggcgg ccgcgtgagc atggaccagg
     3301 tgatccagcg gctcgacgcg tcgggtttcc accgcacggg cacggtcatg gagccgggcg
     3361 aatacgcggt gcgcggcggc attctggacc tcttcgcgcc ggggcacgcg cgccctgtga
     3421 ggctcgattt cttcggcgat acgcttgaaa gcatccggca gttcgatcct gtcacacagc
     3481 gcacgcaggc cgcgcgcgaa caagtcacgc tcctgcctat cagcgagata ccgtcgggcg
     3541 aggcggccat caagcacttc cgccagcgct atgtggagct gttcggcccg gcaaaaggcg
     3601 acgacgcgct ctacgaagcc gtctccgcgg gcagccgcta tcccggcatg gagcattggc
     3661 tgccgctgtt ctacgaacgg atggatacgc tcttcgatta cctgccgaac gcggtcatca
     3721 ctctcgacca tctaggcgac gatgcgacgg cgcggcggct tgaaacgatt gcagaccatt
     3781 atgaggcccg caaagaaggg ctggagcaaa gatcgttcgg cgctcccccc tataagccgt
     3841 tgccaccgga tgcgctctat ttgacggcta tggaatggca gggccatctg gaggcgcgca
     3901 agctgcggct gttcacgcct ttcccccaac cctccccaaa gggccttcag atcgatttca
     3961 gaggcaagca gggccgcagc ttcgcgatcg agcgcacgga atcatccgaa aagctcatcc
     4021 cggccgtcat tgaccatatc cgcacccttc aggggcttgg gaaacgaagc atcgtggcct
     4081 gctggaccaa cggcgctcgc gagcggctcg gcaatatgat ctcggcgcat gagaacattc
     4141 gcatcgagaa ggtcgcgaat atcgccgaag cgaggaaagc tgacaagtcg gcggtgagct
     4201 ttgcgttgct cggcctcgaa agcggctttg agacgccgga cctcgccatc atcgctgagc
     4261 aggatattct aggcgacagg ctcatccgcc gccgaaaggc gaaaaaggca agcgacgtgc
     4321 tggtggaggc ctcaagcctc acgcccggcg atctcatggt gcatgccgat catggcatcg
     4381 gccgcttcat cggcctcaaa actatcgccg cggcgggggc gccgcacgat tgcgtggaac
     4441 tcgaatattt gggcggcgac aagctctttc tgcctatcga gaatatggag cttctgacgc
     4501 ggtacggctc cgacgagacg agcgcgaacc tggacaagct cgggggcgcg tcctggcagg
     4561 gccgcaaggc ccgcatgaag aagcgcattc gtgagatggc ggaggagctg atcaagattg
     4621 cggctgcgcg cgagacaagg ccagcgcccg ttttcactgt gccggatggt gcttataacg
     4681 agttcgtcac gcgcttcccc tatgaggaga ccgacgatca gttcgccagc atcgacgcag
     4741 tcaccgaaga cctcgccaag ggcaagccga tggaccggct catttgcggc gatgtcggct
     4801 tcggaaagac ggaagtggcg ctgcgcgcgg ccttcctagc ggtcatggaa ggaaagcagg
     4861 tcgcggttgt cgttccgacg acgcttctgg cgcggcagca ctttcagacc tttacggatc
     4921 gcttccgcgg ctttcccgtg aaagtggcgc aggcgtcccg gctcgtcgct tcgaaggagc
     4981 tggcacaggt tcgcgaggga ctgaaaaacg ggcagatcga tattgtcgtc ggcacccatg
     5041 cgctgctcgc caagtccatt gggttcaagg atctcggcct tctcatcatc gacgaggagc
     5101 agcatttcgg cgtgggtcac aaggagcggc tgaagtctct tcgcgaagag gtccatgtgc
     5161 tgacgctgac cgcgacaccg atcccgcgca cgctgcaact cgcgctgacc ggtgttcgcg
     5221 agatgtcagt catcgcaact ccgccggtgg accggctcac cgtgcgcacc tttatcacac
     5281 ccttcgatcc cgtgaccctg cgcgaggcgc ttttgcggga gcgctatcgc ggcggccaaa
     5341 gcttctatgt ggtgccgcgc atatcggacc ttgacgaggt cgccacgttc cttcgcgagc
//
LOCUS       DATMGX010000888         3864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_292291, whole genome shotgun sequence.
ACCESSION   DATMGX010000888 DATMGX010000000
VERSION     DATMGX010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292291"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VNR65_14560"
     CDS             <1..539
                     /locus_tag="VNR65_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ19940.1"
                     /translation="IAAWARFLSKVLTGFPSILAGVFVYAAVVLLMGTYSAIAGGIAL
                     AVLMLPTVTLTAEGAMGMVPQKMKDAAIGMGCTKAQVVWSVTLPTALPSVVTGIILAV
                     ARVAGESAPLLFTALFSNYWLRSLREPTASLSILIYNFSSMPFENQIELAWAASLVLV
                     MIVLGFNILSRFIGRPQV"
     gene            551..1372
                     /gene="pstB"
                     /locus_tag="VNR65_14565"
     CDS             551..1372
                     /gene="pstB"
                     /locus_tag="VNR65_14565"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWJ19941.1"
                     /translation="MSYVEVLTPNEAPQKAPEVVIDCDVKKIMYGAFQAVRDSRVPIE
                     KGKITGFIGPSGCGKSTVLRSLNRMNDLIKGFSLAGEVHFLGHNVYAKNIDPVVVRRH
                     IGMVFQQPNPFSMSIFNNVAFGLRLNRFKGDLHEKVEKSLRRAALWDEVKDKLKESGL
                     SLSGGQQQRLCIARAIAPEPTVLLMDEPCSALDPIATRRIEELMLELKKDYTIALVTH
                     NMQQARRVADKTAFFSVDISKGGRTGYLVEMGDTDQIFDAPEAQLTKEYIRGEFS"
     gene            1395..2552
                     /gene="pstS"
                     /locus_tag="VNR65_14570"
     CDS             1395..2552
                     /gene="pstS"
                     /locus_tag="VNR65_14570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWJ19942.1"
                     /translation="MLLPRMLTAQIAAALAGVLSIVAATLIGPAFAGGVELRGAGSTF
                     VAPLMNGWINFFENSQPDVGIHYDAVGSGEGVSRLLSGSVDFAASDNFLPPADAKKID
                     GGVVQVPSTAGMIVLAYNLPGVKGRLKLPQDVYVDIFLGKIQTWNDPRIRAANKDLNL
                     PAINIAVVARQDSSGTTYAFTEHLAAVNNSWSKGPGVGKLVQWPNVTMLARGNEGIAS
                     KIKISQGAIGYIEYGFALRLGLQMAVLENKAGQFVPPTPLSGAAALNPTMDVGLDALD
                     SSTANPPGMKAYPIVTYSWFLLYPSYQPEKARALMSFLNFAYDEGQSYAPYFGYLQLP
                     DPVMQQARAALTRFRANSAAVPEKASVLDKVTATTNGQDTEAPAAAPAGAK"
     gene            2590..3270
                     /locus_tag="VNR65_14575"
     CDS             2590..3270
                     /locus_tag="VNR65_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19943.1"
                     /translation="MSVLNLAAIDSALRELQRVFPQINLSLLDRRDSLDDEVILNMLE
                     GYALVDRLISDRVDIFEIGHSSYWLKLNATVLCGMDPNKLSRHHQMLEATDVRFYEEP
                     GAGIRYVMDWYSLNKGKDVWRRAAGVYSRILSDPQLFIEGNHRTGALIMSYLLAREGQ
                     PPFVLTSKNAKGFFDPSSVVKKTKKRNIFGEIKFKRITREFADFLEAHKNLDFLVTTR
                     MHSKDLRF"
     gene            3437..>3864
                     /locus_tag="VNR65_14580"
     CDS             3437..>3864
                     /locus_tag="VNR65_14580"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ19944.1"
                     /translation="MHDVIFNPVDPLPKVWDPNARIGDMLRGKRGLVVGIANEHSIAF
                     GCAAKLRAFGAELAITYANEKSEPYVRPLGEHIQASFMMPLDVEKPGELEAVFDRIKS
                     EWGRLDFLIHSIAFAPKDDLHGRVVDCSLPGFEKAMRISC"
BASE COUNT          823 a         1110 c         1082 g          849 t
ORIGIN      
        1 ggattgcagc gtgggcccga tttctctcaa aggttctcac gggcttccct tcgatcctcg
       61 ccggcgtgtt tgtctacgcc gccgtggtgc tgctgatggg cacgtactcc gcgatcgccg
      121 gaggtatcgc ccttgccgtc ctgatgctcc ccaccgttac gctgacagcg gaaggagcca
      181 tggggatggt gccgcaaaag atgaaggacg cagccatcgg catgggttgc acgaaagccc
      241 aggtcgtgtg gagcgttacg ctgcctaccg cgctgccgag cgtcgttaca ggcattattc
      301 ttgcggtggc gcgcgtggcc ggcgaatcgg cgccgctcct ctttacggcg ctgttcagca
      361 actactggct gcgcagcctg agagagccaa cagcgtcgct gtcgatcctg atctacaact
      421 tttcgagcat gccgttcgaa aaccagatcg agctcgcatg ggcggcgtcg cttgttctcg
      481 tcatgatcgt tttgggcttc aacattctga gccggttcat aggtcgtccg caggtctgag
      541 gagttagaac atgtcttacg ttgaggttct cacacctaat gaggcacccc agaaagcgcc
      601 ggaagtcgtc atcgactgtg acgtgaagaa gatcatgtac ggcgccttcc aggccgtgcg
      661 cgatagccgt gtgccgatcg agaaggggaa gatcaccggc ttcatcggcc cgtcgggctg
      721 cggcaagagc accgttctgc gcagcctcaa tcgcatgaac gatctgatca aaggcttcag
      781 cctcgctgga gaggtgcatt tcctcggcca taatgtctat gcgaaaaaca tcgatcccgt
      841 cgttgtccgg cgtcacatcg gcatggtctt tcagcagccc aaccccttct caatgagcat
      901 cttcaacaat gtcgccttcg gcttgaggct caatcgcttc aagggcgacc tccatgaaaa
      961 ggtcgagaaa tcgcttcgcc gcgccgcact ttgggacgag gtgaaagaca agctgaagga
     1021 aagcgggctc tcgctctcag gcggccagca gcagcgcctt tgcatcgccc gcgccatcgc
     1081 cccggaaccg actgtgctgc tgatggatga gccatgctcg gccctcgatc cgatcgcgac
     1141 gaggcgcatc gaagagctga tgctcgagct taagaaggac tacaccattg cgcttgtcac
     1201 ccacaacatg cagcaagcgc gccgtgtagc agacaagacc gctttcttct ccgtcgacat
     1261 ctcgaaaggc ggccggacgg gctatctggt tgagatgggc gacaccgatc agatcttcga
     1321 cgcgccagaa gcgcaactga ccaaggaata cattcggggc gagttcagct aagctaattt
     1381 tagaggatcg aataatgctt ctcccgagaa tgttgacggc tcaaattgcg gctgcgctag
     1441 cgggcgtact ttcgatagtt gctgcaaccc tgatagggcc agcctttgct ggcggggtgg
     1501 aactccgtgg ggcaggctcg acttttgtgg ccccgctcat gaatggctgg atcaacttct
     1561 tcgagaattc ccagcctgac gttggcattc actatgatgc cgtcggctcc ggcgaaggtg
     1621 tttcgcgctt gctgtccggt tccgtcgatt ttgcggccag cgataatttc cttccgccgg
     1681 ccgacgcaaa gaaaatcgac ggcggcgtcg tgcaagtgcc gagcacggcg ggcatgattg
     1741 tcctcgccta taatcttcca ggcgtaaaag gccggctcaa actcccgcaa gacgtttacg
     1801 tcgacatctt tctcggaaag atccagacct ggaacgatcc gcgcattcgc gccgcgaata
     1861 aggatctcaa tctgccggcc atcaatatcg ccgttgtggc ccggcaggac tccagcggaa
     1921 ccacctacgc ttttacggag catcttgcgg ctgtgaataa ctcctggtcg aaagggccgg
     1981 gcgtcggcaa acttgtccaa tggccgaacg tcacgatgct cgcgcgcggg aacgagggca
     2041 tagcttcaaa gatcaaaata agccaaggcg caatcggata catcgaatac ggcttcgcgc
     2101 ttcgccttgg gttgcagatg gccgtgctcg agaacaaggc tgggcaattc gttccgccta
     2161 cgcccctatc cggagctgcc gcgcttaacc cgacgatgga cgtaggcctt gatgcactgg
     2221 attcgtcaac ggctaaccca cccggcatga aagcctaccc gatcgtcacc tatagctggt
     2281 ttctcctcta tcccagctac cagccggaga aggctcgcgc cttgatgtcc ttcctgaact
     2341 tcgcctacga cgaagggcag agctatgcgc cttatttcgg gtatttgcag ctccctgatc
     2401 ccgttatgca gcaggcgaga gcagcgctga cgcgttttcg tgccaatagc gccgcagttc
     2461 ctgaaaaagc ttctgttctc gataaggtga ctgcaacgac gaacgggcaa gatacagaag
     2521 cgcccgctgc cgctccggcg ggggccaagt aagtacacat ccttttgact atacagcctg
     2581 gaggccgagg tgagcgtact taatctcgcc gcgatcgact cggcccttcg ggaactccag
     2641 cgcgttttcc cgcagatcaa ccttagcctc ctcgaccgcc gcgattccct tgatgacgag
     2701 gtgatcctca acatgctcga agggtatgcc ctcgtcgatc ggctgatctc ggaccgggta
     2761 gacatcttcg agatcggtca ttccagctat tggctaaagc tgaacgccac cgttctttgc
     2821 gggatggacc cgaacaaact atcgaggcat catcagatgt tggaagcgac tgatgttcgc
     2881 ttctacgagg agccgggcgc agggatccgc tacgttatgg actggtattc cctgaacaag
     2941 ggcaaagacg tctggcgccg ggcggctggc gtctacagtc gcattttgag cgaccctcaa
     3001 ctcttcatcg agggcaatca taggaccggc gctctgatca tgtcctacct gcttgcccgt
     3061 gagggccagc cgccgtttgt tttgacaagc aagaacgcaa aaggattttt tgatccgtct
     3121 tctgtagtca agaaaaccaa gaagcggaat atttttggtg agatcaagtt taagaggata
     3181 acacgagagt ttgctgattt ccttgaagcc cataaaaatc tcgattttct tgtcaccaca
     3241 agaatgcatt caaaagatct acgattttaa caccgtttct actcctcctc ccatgcgcta
     3301 aaaagatccc gtcctacgtt cccccaaaga ttcattgcga tgcgcgtcac gaagacgtca
     3361 tgggttgtga ttagcatcgc tcaagatggt gggcgctccg agagcgagca ctccgggtcg
     3421 tatgcaggag gccattatgc acgacgtcat tttcaaccca gttgatcctc tgcctaaggt
     3481 ttgggacccg aatgcccgca tcggagacat gctgcgcggc aagcgcgggc tcgtcgtagg
     3541 gatagcaaac gagcacagca tcgcgtttgg ctgcgcggcc aagctcaggg ccttcggcgc
     3601 tgaactggcg atcacttatg cgaacgagaa atcggagccg tatgtccgtc ctcttggtga
     3661 gcacatccag gcaagtttca tgatgccgct cgacgtcgaa aagccgggag aactcgaagc
     3721 tgtcttcgac cgcatcaaga gtgagtgggg ccgtctcgat tttctgattc actcgatcgc
     3781 gttcgcaccc aaagacgatt tgcatggccg ggtggtcgat tgctcgcttc caggtttcga
     3841 aaaagcgatg cggatatcct gcta
//
LOCUS       DATMGX010000889         2421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_51238, whole genome shotgun sequence.
ACCESSION   DATMGX010000889 DATMGX010000000
VERSION     DATMGX010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51238"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VNR65_14585"
     CDS             complement(<1..211)
                     /locus_tag="VNR65_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19945.1"
                     /translation="MAAKLKTAVLGATGYSGLELTRILLRHPRLEKPFLLRRSADNGT
                     ASDLADVFPALYGNGGYPLHALSWPA"
     gene            complement(211..654)
                     /gene="argR"
                     /locus_tag="VNR65_14590"
     CDS             complement(211..654)
                     /gene="argR"
                     /locus_tag="VNR65_14590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HWJ19946.1"
                     /translation="MNKTLRQRAVLDVLKHGSIASQEDLQRLLRKQGFKVGQATLSRD
                     IRDLNLSKTSHGYSLPQGESGAGVALPPVSRLVREFVLDIRTAQNQLVIKTIVGSAQP
                     VAAALDEADWSEVVGTIAGDDTILIVCPDKDDARKLAARIEEMLG"
     gene            872..1648
                     /locus_tag="VNR65_14595"
     CDS             872..1648
                     /locus_tag="VNR65_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HWJ19947.1"
                     /translation="MNPKPSLIAELIAEFLGTFVLILFGTGVVAMVVLFPSRNPGETI
                     HGGFTNITLGWGLGVTMGIYIAGKVSGAHLNPAVTLAFAVFRGFPWRKVVPYSIAQTA
                     GAFAAASLVYWNYLPAFRQTDPQLDHTAGVFTTFPAFPAILEAGFLDQLIGTALLLLL
                     VFAIIDEFNSPPGANIAPAMIGLVVVAIGMSFGGMHGYAINPARDFGPRLFTVVAGFH
                     NNGFTDGTRVWWIPVVAPLLGGLLGAALYDFGIRRFLRPS"
     gene            1688..>2421
                     /locus_tag="VNR65_14600"
     CDS             1688..>2421
                     /locus_tag="VNR65_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWJ19948.1"
                     /translation="MKYVLALDQGTTSSRALLFDEDGVVRAVAQREFDQIFPQPGWVE
                     HNPDQIASSQIAVALESLAQVKAQPSDIAAIGIANQRETTIVWDRETGEPVYNAIVWQ
                     DRRTADFCERLRSEGHGPLIQQRTGLLIDSYFSASKISWILENVPGARSRAEAGQLAF
                     GTVDTWLLWKLTAGRVHATDASNASRTMLFNIHTGAWDHELLDLFRVPLSMMPAVRSS
                     SEVYGEVSAVQGLERIPLGGIAGDQQA"
BASE COUNT          469 a          795 c          665 g          492 t
ORIGIN      
        1 tcgctggcca cgaaagcgcg tgcagcggat acccgccgtt gccatagagt gcgggaaaca
       61 catccgccaa gtccgaagca gtgccattgt ccgccgaccg gcgaagtaga aaaggcttct
      121 ccagccgcgg atgacgcaga agaattcgcg tcaactccag gccggaatat ccagtcgcgc
      181 ccaacacggc ggtcttgagt ttcgcggcca ctagcccagc atctcctcaa tccgggccgc
      241 caacttccgg gcgtcatcct tatcgggaca cacaatcaga atggtgtcgt cgccggcaat
      301 cgtcccgacc acctcggacc aatcggcctc gtccaacgcc gccgccaccg gctgcgcgct
      361 gcccacaatg gtcttgatca ccaactggtt ctgcgccgtt cgaatgtcga gcacaaattc
      421 ccgcaccagt cgcgacaccg gaggcagcgc aactcccgcc ccactctcgc cctgcggcag
      481 ggaatagccg tgggaggtct tgctcagatt cagatcgcgg atgtcccggg acagcgtagc
      541 ctgcccgacc ttaaagccct gcttcctcag cagccgctga agatcctcct ggctggcgat
      601 ggacccgtgc ttgagcacat ccagaaccgc ccgttgccgc agtgttttat tcataatgaa
      661 taaatatgca tgctaatgca taaatatttc tatatcttac ggacccctcc ccaattgtca
      721 agcgaaaagg tccgcacagg ttactttccg aggcctgatg ccaggacgaa aaagtcaggg
      781 ctcgccgctg aaaaacgcac gcacggcgcc gccgcttggt agactacttc gcggtctgga
      841 agcgcagtcc cgtcgagcac cggagatgcg aatgaacccg aaaccctcac tgatagcgga
      901 gttgatcgct gagttcctcg gcacttttgt cctcatcctg tttggcacag gagtagttgc
      961 catggtcgtc ctttttccat cccgcaaccc gggcgaaacc atccacggcg gcttcacaaa
     1021 tatcactctg ggatggggac tcggggtgac gatggggatt tacatagcag gcaaggtgtc
     1081 gggggcgcat ctcaatcccg cggtgacctt ggccttcgcg gtttttcgtg gcttcccatg
     1141 gcgcaaggtt gtgccctatt ccatcgcgca gaccgccggg gcgttcgcgg ccgcgtccct
     1201 tgtgtattgg aactacttgc cagcgttccg gcagaccgat ccccaactgg accacactgc
     1261 cggcgtattc acgacttttc cggccttccc tgcgatcctg gaggccgggt ttctggatca
     1321 actgatcggc accgcactgc tgctccttct ggttttcgcc atcatcgacg aattcaactc
     1381 gcccccagga gcgaacatcg cgcctgcaat gattgggctg gtcgtggttg ccattggcat
     1441 gagtttcgga ggcatgcatg gctatgccat caatcccgcg cgcgacttcg gccccaggct
     1501 cttcacggtg gtcgccggat ttcacaacaa cggcttcacc gacggaaccc gcgtatggtg
     1561 gattcccgtc gtcgctccac tgctcggcgg cctgctggga gcggctctct atgactttgg
     1621 gatccggcgc ttcctgcgcc cttcctagcc aactctcagc gcaccgcccg atgttcgagg
     1681 aggaactgtg aaatatgttt tggcgttgga ccagggcacg accagctcgc gagctctctt
     1741 gtttgacgag gacggcgtcg ttcgcgccgt cgctcaacgg gaattcgacc aaatctttcc
     1801 tcagcccgga tgggtcgagc acaatcccga tcaaatcgct tcctcccaga ttgcggtagc
     1861 cttggaatcc ctcgctcaag tcaaggcgca gcccagcgac atcgccgcga tcggcattgc
     1921 taaccagcgt gagaccacga ttgtgtggga tcgcgagacc ggagaacccg tctacaacgc
     1981 catcgtctgg caagaccgcc gcaccgccga tttctgcgaa cgcctccgca gtgaaggcca
     2041 cggcccgctc atccagcagc gcaccggact gctcattgat tcctatttct cggcaagcaa
     2101 aatctcctgg atcttagaaa atgttcccgg tgcgcgcagc cgcgcggaag cgggccagct
     2161 cgccttcggc accgtcgata cttggctcct ctggaaactg accgccggcc gcgttcacgc
     2221 gaccgacgcc agcaatgcct cgcgcaccat gctcttcaac atccacaccg gcgcctggga
     2281 tcacgaactc ctcgatctgt ttcgcgttcc gctctcgatg atgccggccg tgcgttcctc
     2341 cagcgaggtc tacggggaag tcagcgcggt acagggattg gaaagaattc cgctcggcgg
     2401 catcgccgga gatcagcagg c
//
LOCUS       DATMGX010000890         3933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_143019, whole genome shotgun sequence.
ACCESSION   DATMGX010000890 DATMGX010000000
VERSION     DATMGX010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143019"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            427..594
                     /locus_tag="VNR65_14605"
     CDS             427..594
                     /locus_tag="VNR65_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19949.1"
                     /translation="MIQLSLIETDPPALVQAAWEQFDPTYREALTRRLALVIAKVVAT
                     PAVAEDKSDDE"
     gene            587..2800
                     /locus_tag="VNR65_14610"
     CDS             587..2800
                     /locus_tag="VNR65_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWJ19950.1"
                     /translation="MSEITKVTPSHVSRLAIVYVRQSSATQVENNRESTARQYALKQR
                     AEALGWSVDRIVVIDDDLGLSGATAHQRNGFARLAAEVALAHVGIVLGLEVSRLARNN
                     TDWYRLLDLCSMTNTLIGDADGVYHPSLFNDRLLLGLKGTMSEAELHVLRARLDGGIR
                     NKAARGELRRGLPIGFVWGEADGEVLLHPDEAVRAAVRAVFERFQEFGSARRVWLWFR
                     SEGLDFPLQIQASEPIRWVAPTYTAIYNILRNPVYAGAYVYGKSRREHTFDEHGNRHK
                     RTRLLPREQWAVLLRAHHEGYIDWPTFEANEVRLAANIHPQSHRAGAGPLREGAALLQ
                     GLAVCGHCGRKLHTHYRGRLQTPGYHCSNKGIENGRGVYCLNVGGLQIDAAVTQAFLS
                     AIAPAGLLAVVEAAEHLQADHEGALAQWRLALERAEYDAQRAERRYRAVDPENRLVAR
                     GVEAQWEESLRVVQRARAELTRRQEQRPHAVTDEQRRRLLCLGEDLQLVWNAPTTSLR
                     DKKELLRTLLEEVSVSVYREEFRALLKLRWRGGMLNEFDVSLPRSRPATVRTDEDTVA
                     LVQRLAQYHDDAAIAGILSRQERTTARGLRFTQNHVGNLRRSWDIPRCTKSKDSSAGD
                     LMNIRQTAKELGIAASTIHRWLKDGFIVGEQITPGAPWQIRVNDALRRRLVQAAPEGY
                     LAMQEATKLLGVTRQTVLQRVKRGELEAVLLHEGRRKGLRIKVSNANPGLFERTS"
BASE COUNT          798 a         1197 c         1121 g          817 t
ORIGIN      
        1 cttgggcgct gtcagccatc gttggatgta gagcagaacc cacggaaccg tcgtctgttt
       61 ctttaccgcg cgcatcaaca actcatgctc gattgtctcg aatgaaacca cgacacacta
      121 cccatctcca ggacattccg caaacggtac gcgttgtgcg cagcggccac gcgtttgaag
      181 gccacgagct tgcagtgatc agccgtgccc gacggggcgg ccaactgtac ttactgctaa
      241 agctgccgga tcaatcgcgc tctctcattc ccgcaagctg gaccgacctg cgagcgcggg
      301 ccgatgtggc acccgtcaac ggcgccacgc caccgcaagc actactcgcc acgatggagg
      361 atttactcaa agcgcgtaac gtcatcgaca ctatgcttag tcgcaagacc gacgcaaagg
      421 agctcgatga tacagctcag cctgatcgag acagatccgc ctgccctggt tcaagcagcg
      481 tgggagcagt tcgacccgac ttatcgcgag gcgctcacaa ggcgcctcgc tctcgtcatc
      541 gccaaggtgg tcgctacgcc ggcggttgcg gaggacaaga gtgacgatga gtgagatcac
      601 caaagtcacg ccttcacacg tgagtcgcct ggcgatcgtc tacgtacgtc agtcatcggc
      661 cacgcaggtc gagaacaatc gcgagtccac cgcgagacaa tacgcgctca aacaacgcgc
      721 cgaggcgttg ggctggtccg tagatcggat cgtcgtcatc gacgacgatc tcggcctatc
      781 aggcgcgacg gcgcatcagc gcaacggctt cgcgcgtttg gctgccgaag tggcgctcgc
      841 gcacgttggc atcgtcctgg ggcttgaggt ctcgcgcctg gcacgcaata acaccgattg
      901 gtatcgcctg ctggacctgt gctcgatgac caacacgctc atcggcgacg cggacggcgt
      961 ttatcatccg tcgttgttca acgatcgcct cttgctggga ttgaagggca ccatgagcga
     1021 agcggaattg catgtgctcc gcgcccgcct ggacggcggc attcgcaaca aagccgcgcg
     1081 cggcgagctg cgccgtggct tgccgatcgg cttcgtttgg ggcgaagccg acggcgaagt
     1141 actgctccat cctgacgaag ccgttcgggc tgcggtacgc gcggttttcg agcggtttca
     1201 agagttcggc tcggcgcgtc gcgtgtggtt atggtttcgc agcgaaggtt tggattttcc
     1261 attgcagatt caggcctcag aacctattcg ttgggttgcg ccaacctata ccgctattta
     1321 caacatcctt cgcaatccgg tctatgcggg cgcatacgtc tatggcaagt cgcggcgaga
     1381 gcacaccttc gacgaacacg gcaaccgtca caaacgtact cgccttttgc cgcgtgagca
     1441 gtgggcggta ctccttcgtg cgcaccacga aggctatatc gattggccca cgtttgaagc
     1501 caacgaggtg cgtttggccg caaacataca ccctcaatcc caccgagcag gagcaggacc
     1561 attgagagaa ggggctgcgc tcttacaggg actcgccgtc tgcggtcatt gcggacgaaa
     1621 gctgcacacc cactatcggg gtcggctaca aacgcccggc tatcattgct ccaacaaagg
     1681 tatcgagaac gggcgcggcg tgtattgcct caacgtcgga ggtctgcaaa tcgacgcggc
     1741 cgttacacag gcatttctct cagccatcgc tcccgcagga ctgctcgcag tggttgaggc
     1801 agcggagcac cttcaggccg accacgaggg ggctcttgca cagtggcgtc ttgcgctcga
     1861 gcgcgctgag tatgatgcac agcgcgcaga gcgacgttac cgcgctgtcg atcccgagaa
     1921 tcgtctggtc gcgcgtggtg tcgaagccca gtgggaggaa agtttacgtg tcgtgcaacg
     1981 tgcacgcgct gaactcacac gtcgccaaga gcaacggccg catgcagtga ccgacgagca
     2041 gcggcggcga ttgctctgtt tgggcgaaga tctccagctg gtctggaacg ctccgaccac
     2101 atcgttacgc gacaagaaag aactgctgcg aacgctgctc gaagaggtca gtgtcagtgt
     2161 gtatcgcgag gagtttcgcg cgcttctgaa attgcgctgg cgcggcggca tgctcaacga
     2221 attcgacgtg agcctgccgc gctcgcgtcc agccacggtg cgtaccgacg aagacacggt
     2281 tgcccttgtg caacgcctcg cgcagtatca cgacgacgca gccatcgctg gaattttaag
     2341 tcgacaagag cgtaccaccg cgcgtggtct acgcttcacg cagaaccatg tcggtaactt
     2401 gcgccgcagc tgggatatcc cgcgctgcac taaatcaaaa gactcttcgg ccggcgacct
     2461 tatgaacatc aggcagacgg caaaagaact cggcatcgcc gcctctacca tccatcgttg
     2521 gctcaaggat ggcttcatcg tcggcgagca aatcacgcca ggcgcgccgt ggcaaatccg
     2581 cgtcaacgac gccttgcggc ggcgtttggt gcaagcagct cccgaaggct atcttgcgat
     2641 gcaagaggca acaaaacttc tcggcgtaac acgacagacg gtgttgcagc gtgtaaagcg
     2701 cggcgaactc gaggccgtgc ttctccacga agggagacga aaaggcttga ggataaaggt
     2761 ttctaacgca aatcccggtc tatttgaacg cacatcataa accagggggc aatgtaaaca
     2821 tggatccaag tatcgagata gatcgacatc aatgaccgtg aacatgcgtc gctgcacact
     2881 gcgtctgact tctgccagag cgcggtgagg tgaccgttga ggtcggaatc cgtaggagtt
     2941 cgggcagaaa tccgcctcga agatcgcttc gagtatcagc ttcaccgctc cctgcaccac
     3001 gcgatcgcga atgctgggaa tttgaagtgt gcggaccttg ccgttggcct ttggaatgtc
     3061 aacccgacgg ttcggcttgg gtttgtatct acctgatacg agttcttccc gtatttccgc
     3121 aagaaatgcg tcaagtcccg atgcctcgac gtcggcgaag ctttgacgat cgatgccggg
     3181 ggctccacga tttgttttgg cgatacggta ggactctttg agggtttcca tctttgtgat
     3241 atgcacaaag agcccccaga aacgatggga cttgtttgac ttcgtctttc gataaatccg
     3301 ccgccgcagc tcctgcagat ccgtgggtgc ctttatcatc tcacccctgc ctcgccttga
     3361 cgtacggaac actcacaggt ccgggccctt ccctccggac gcgttttagt gcacgtccat
     3421 caacggtatt acgacccgat ccgccaccct gatgcctacg ccggcatttc ccactcgcgg
     3481 ttatagccgt catctcctcg gcaagctttc cttgccggac atcgagggct tctccagttg
     3541 acactccatc cttcacacca tgtcgccgct gataccccgc cggtgagagc cgccgtttcg
     3601 gactcgtttc gattcggctc ttgttgcttt cgctctgtgg cgcgaccgtc tcagccaccg
     3661 aattttggta agtaacgagg ctacatctac gttcactgca cgttgcaacc tggtgcgttg
     3721 catagctcct ttcgagccat gtgtcggagg gctccgccgc cttgctttcg cgccacgacg
     3781 cctcccaagc tacgagactt gagtcttttg gtctcggtcg gactttcacc gactggatgt
     3841 tctgcccttg actggacacg ccacgccccc tatacgccgg ccgccgcccg cccagtaatc
     3901 aggtttccgg taggcttgtc ccaggagaac tac
//
LOCUS       DATMGX010000891         3857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_180463, whole genome shotgun sequence.
ACCESSION   DATMGX010000891 DATMGX010000000
VERSION     DATMGX010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180463"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VNR65_14615"
     CDS             complement(<1..1043)
                     /locus_tag="VNR65_14615"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWJ19951.1"
                     /translation="MDPRLKAFKSPDFDRVFGAVASLAGAADEERVDVPEIEAEASDA
                     LTAIVAQARAQNPDPASRILLIKGATGTGKTHTLLTAIRRMHQEGNVYAALFPMVDLV
                     AEKDLDAWLLRAMVSRLSERYLVAADAPSPLARLAAAVLQRAQPDLMAAFRRDVWRNE
                     GDIRDFDLRPLVVSIRLKLQKLSHLPVPSEAFVTALLGAAAGDDDCLAYLRGQPLNVS
                     LGGVRLSQLAEDHFARSHIDALVSVVGATGGALVLAFDQLEQSRVQGWEQRLRHLFSR
                     GALLAETLPPLCVAYAVLPALYDTISDGIDGSIRDRIERFGALPVRLRPLARRQVEAL
                     LKRRLAELFERSGT"
     gene            complement(1054..2013)
                     /locus_tag="VNR65_14620"
     CDS             complement(1054..2013)
                     /locus_tag="VNR65_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19952.1"
                     /translation="MMTKEDISRLPKAAAGCLHPLETAEIAAAFVAAAHRDGCSRADA
                     ASGLSELFAAEPVLDEALRLGLVQERGGLLYAPGRPSNWADARREFAFRALGFPASDN
                     THRELAGAALRLLYGLNNLPPSPSRAQARCELLRRIVASFGEELVAGVGQPGKAFSFD
                     PMSRSIYLAFAGLDSGTVLQADAALLSSAFGGPASTIAELSTTILRAALHANVTDAAK
                     REEVFDLDGFAESVRKIAGKLETKPYAGRVAIAQVYDAGLAQGLELGTLDDFKSHVAE
                     AAREGLLDLERYDIAGPFDAGLKERSRLRFGRDERHFIVNQWI"
     gene            2310..3164
                     /locus_tag="VNR65_14625"
     CDS             2310..3164
                     /locus_tag="VNR65_14625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWJ19953.1"
                     /translation="MTNRILCIGAFALALGAQLAYAGPVAKVNGTDITDAELAFAEAE
                     VGAEIAGLPAESRRRVLLEYLIEAHLFAEEANKGQLTSGKEFEDRLSYYKLRAMRDTF
                     YEKKVRGGVSDTQARAAYDEQIGKLKPEAEVHARHILVKTEDEAKDLVKQLRAGGDFN
                     ELAKKSSDGPSSHTGGDLGYFSRGQMVKSFEDAAFALEPGQVSDPIKSEFGWHVIKVE
                     DKRNRPAPSFEEVKDQITASLIQAQLKDTVQKLRSSASVDVIDPELKKAMEDDAKAAT
                     LEPQTQQK"
     gene            3188..>3857
                     /locus_tag="VNR65_14630"
     CDS             3188..>3857
                     /locus_tag="VNR65_14630"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HWJ19954.1"
                     /translation="MANIQKVSPFAPTELVDLPAINGVRLAVAEAGIKYANRTDVLLM
                     VFPEGTEVGGVLTRSKTCSAPVDWCREQLQHGSGRALVVNSGNANAFTGQRGRETVET
                     TATFAAEAAGCEPDRVFIASTGVIGEPLDASKFKDVLKEMAPRAREDLWADAARAIMT
                     TDTFPKLATRRALVGNVPVTLNGIAKGSGMIAPDMATMLSFIVTDAPIEGHVLTRLLQ
                     QHAKS"
BASE COUNT          827 a         1175 c         1192 g          663 t
ORIGIN      
        1 gtcccggagc gctcgaacaa ctctgcgagc ctgcgcttga ggagcgcttc gacctgacgg
       61 cgcgcgagcg gacgcagccg cacgggaagc gcgccgaaac gttcaatgcg atcgcggatc
      121 gatccatcga tcccatctga aatcgtatcg taaagcgcgg gcaaaaccgc gtaggcaacg
      181 caaagcggag gaagcgtttc cgcaagaagc gcgccccggc tgaagagatg ccggagccgc
      241 tgctcccagc cttgaacccg gctctgctca agctgatcga aggcgagaac gagcgcaccg
      301 cccgttgcgc cgacaacgct caccagcgca tcgatgtggc tccgcgcgaa gtgatcctcg
      361 gcgagctgcg agagacgcac cccgcccagg ctgacattca gcggctgccc gcgcagatag
      421 gcaaggcaat cgtcgtcgcc tgccgcagcg ccgaggagcg ccgtcacgaa agcctcggac
      481 ggaaccggaa gatgcgaaag cttctgaagt ttcaaacgaa tggaaacgac aagcggccgg
      541 agatcgaaat cccggatgtc gccttcgttt ctccagacat cgcgccggaa cgcggccatc
      601 aggtccggct gggctcgctg aagtacggcc gccgcaagcc gcgccaaagg cgacggcgca
      661 tccgccgcga caagatagcg ttcggagagc cgtgaaacca tcgcacgcaa aagccaggcg
      721 tcgagatcct tctcggcgac aagatcgacc atcgggaaaa gcgccgcgta aacgttgcct
      781 tcctgatgca tgcgacggat tgcggtcaga agcgtatgcg tctttcccgt gcccgtcgca
      841 cctttgataa gaaggatacg cgacgctgga tcgggatttt gtgcccgcgc ctgcgcgacg
      901 atggccgtca gcgcatcgct ggcctcggcc tcgatctccg gcacgtccac gcgctcttcg
      961 tccgccgcgc ccgcaagcga agccaccgcg ccaaaaacgc ggtcgaagtc gggacttttg
     1021 aaagccttga gacgagggtc cattgtgatg gcgttaaatc cattgattga cgatgaaatg
     1081 ccgctcatcg cgcccaaaac gcaaacgcga ccgctctttc aggcccgcat cgaacggccc
     1141 cgcaatgtcg taacgctcga ggtcgagaag cccctcgcgc gccgcctcgg cgacgtggga
     1201 cttgaagtca tcgagcgtgc cgagttccaa gccctgtgcg agccccgcat cgtaaacctg
     1261 tgcgatggcg acacgcccgg catagggctt tgtctcgagt tttcctgcaa tctttctgac
     1321 cgattcagcg aatccgtcca agtcaaagac ctcctcacgt tttgccgcgt ccgtcacgtt
     1381 tgcgtgcaga gcggcgcgaa ggatcgttgt gctgagctcg gcgatggttg atgccggacc
     1441 gccgaaggcc gatgacagca gagccgcatc ggcctgcaaa accgtgccgg aatcaagccc
     1501 ggcaaaggcc agatagatgc tgcggctcat cggatcgaag gagaacgctt tgccgggttg
     1561 ccccacgccc gcaacaagtt cctccccaaa tgaggcaacg atgcgccgga gaagctcgca
     1621 gcgagcctgc gcccgcgatg gcgaaggcgg caaattattc agaccataaa gcaggcgtaa
     1681 cgcggccccg gcgagttctc tgtgtgtgtt gtcggatgcc gggaagccca aggcacggaa
     1741 agcgaactcg cgtctggcat cggcccagtt ggacggtctg cccggcgcgt aaaggaggcc
     1801 accccgctcc tggacgagcc ctaggcgcaa ggcctcatcg agaacgggct cggctgcgaa
     1861 caactcagag agcccgcttg ctgcatctgc ccggctgcat ccgtccctat gtgccgccgc
     1921 cacaaaagca gccgcaatct cggccgtttc gagcggatga aggcatccgg cggcggcttt
     1981 tggcagacgg gagatatctt cttttgtcat catcctgcaa ttttggcggg cgagaaggct
     2041 agcccgcgca gcctaacctt ctgtcgaaat taaccgattc gtaacgttct ggaaccagca
     2101 agggcaatgc atacgttgct tcgtcacccg caaactcaac cgaggaaagg gcgactcaag
     2161 tcttgcctgc tttctacaaa ggccctaata gacgggtgaa cagacttccg atccagcgcg
     2221 cgctgtataa aaagctggca gaaaacatgg tgtgcgcggc ggacgcatct aaatttcatg
     2281 tttcccgaaa aggcgaggag ttctcagccg tgacgaacag gattttatgc attggcgctt
     2341 tcgcgctggc gcttggcgca caactcgcct acgcgggccc cgtcgccaaa gtcaatggaa
     2401 cggacattac cgacgcagaa ctcgcctttg cggaagccga ggttggagcc gagatcgcgg
     2461 ggcttcccgc cgagagccgc cgccgcgtgc tgttggaata tctcatcgag gcgcatctct
     2521 tcgcggaaga ggccaataaa ggccagctta cctcgggcaa ggagttcgag gatcggcttt
     2581 cgtattataa gctgcgggcg atgcgcgaca ccttctatga gaagaaggtt cgcggcggtg
     2641 tcagcgacac gcaggccagg gccgcttacg acgagcagat cggaaagctg aagcctgagg
     2701 cggaagtgca cgcccgtcac attctcgtga agacggagga tgaagcgaag gacctcgtga
     2761 agcagctcag ggcgggcggc gatttcaacg agctggccaa gaaaagctcg gacggaccga
     2821 gttcccatac cggcggcgat cttggctact tctcgcgcgg gcaaatggtc aagtctttcg
     2881 aggatgccgc tttcgcgctc gagcccggac aggtttccga tcccatcaag tccgagtttg
     2941 gctggcatgt gatcaaggtt gaagacaagc gcaatcggcc ggcaccaagt tttgaggagg
     3001 tcaaggatca gatcacggct tctctgatcc aggcccagct caaggatacg gtgcagaagc
     3061 tccggtcgtc cgcctcggtt gacgttatcg atccggaatt gaagaaggct atggaggacg
     3121 acgccaaggc agcgaccctc gaaccccaga cccagcagaa ataacaagag cggagccgcc
     3181 tcgcatcatg gcgaacattc aaaaggtttc gccgttcgcg ccaaccgaac tcgtcgacct
     3241 gcctgcgata aacggagtgc ggctcgcggt ggccgaggcg ggtatcaagt atgcgaaccg
     3301 caccgacgtg ttgttgatgg tgtttccgga aggcacggaa gtaggcggcg tcctgacccg
     3361 ttcgaaaaca tgctcggcac cggtggactg gtgccgggag cagcttcagc acggcagcgg
     3421 gcgcgcgctt gtcgtcaatt ccggcaatgc aaacgctttt acggggcagc gcgggcgtga
     3481 gacggttgag accacagcga cctttgcggc cgaagctgcg ggatgcgagc cggatcgcgt
     3541 tttcatcgcg tccacaggcg tcatcgggga gcctctcgat gcctccaagt tcaaggatgt
     3601 gctgaaagag atggcgccgc gtgcccgcga agatctctgg gcggacgccg cgcgcgcgat
     3661 catgaccacc gacacctttc cgaagctcgc cacgcgccgc gcgctcgtcg gcaatgtgcc
     3721 cgtcacgctg aacgggatcg ccaaaggctc cggcatgatc gcgccggaca tggcgaccat
     3781 gctgtccttc atcgtcacgg atgcgccgat cgaaggacac gtgctgacgc ggcttttgca
     3841 gcagcacgcg aagtcga
//
LOCUS       DATMGX010000892         3330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_436880, whole genome shotgun sequence.
ACCESSION   DATMGX010000892 DATMGX010000000
VERSION     DATMGX010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436880"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="VNR65_14635"
     CDS             complement(<1..1900)
                     /locus_tag="VNR65_14635"
                     /inference="COORDINATES: protein motif:HMM:NF039222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWJ19955.1"
                     /translation="MTIRNLNLLFQPRSVALVGASERPGSIGLHVAENLLRGGFAGEI
                     GFVNPGHDAILGKPCHRSIESLPFVPGLAVIATPPNTVPGIIDELGRKGCKAAVVITA
                     GITRDLAQRMQAAAKPFLLRIIGPNCMGIQIPSLKLDASFAHTLAKPGDIALVSQSGA
                     ITLAMLDWAAVEGVGFSHVVSIGDCADVDLGDMLDYLAGDITNRAVFLYIESVKDAAK
                     FMSAARRCSRAKPVIAIKAGRHPEGAKAALSHTGALAGSDNVYTAALRRAGILRVVDL
                     DEMFDAGEVLARVRCIPGSRIAIVTNGGGAGVVAADTVADLRGTLADLEPATIDALNK
                     TLPPTWSHGNPVDIIGDAGPDRYAAAMEAVMQDPNTDVVLVINCPTALASSVDAAKAT
                     IEAVEKQRRLGHKKPVVATWLAKSAVEEVRPLFAKANIPDFETPTEAIEGIMQLARYA
                     RAQTELMRTPPVTPENHVDPAAVDALIAHTLGQGRSMMTEPEAKAVLSAYGIPTVPTR
                     VAATPEEAARAAAELLRDYASVVVKILAPNLTHKSDIGGVRLDITSSEEVEKATRRML
                     SEIKAAHPDAIIEGVTVQPMIHRKDAYELILGISTDDVFGPVIVFGAGGTAVEAIGDS
                     AMSLTPLDL"
     gene            complement(2063..2398)
                     /locus_tag="VNR65_14640"
     CDS             complement(2063..2398)
                     /locus_tag="VNR65_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19956.1"
                     /translation="MDQVQPPLYALFAAIFGYLREAWLWWLGQVLSVPWGRIGELPGS
                     KVLLLIASAGIVAYFLYRAARELYSAGAKAFSAFVTLLRVFAKTVWPLLFAALASAAG
                     AWVVNHVQL"
     gene            2686..3300
                     /locus_tag="VNR65_14645"
     CDS             2686..3300
                     /locus_tag="VNR65_14645"
                     /inference="COORDINATES: protein motif:HMM:NF001268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HWJ19957.1"
                     /translation="MPRRLLNIGPLKTDGYLDGQLLIAMPSMTDSRFHRAVIYMCAHS
                     AEGAMGLVINQRAPNITLPRLLEQLEIIPEGRRSKAIRVSSMAVHMGGPVETSRGFVL
                     HSADYFAAESTLPINEGVCLTATIDILKAIAKGSGPNKAILALGYAGWAPGQLEHEIQ
                     TNGWLNCPADPDIIFNSDLESKYGRALDSLGIDPSHLVGVAGRA"
BASE COUNT          685 a         1002 c          997 g          646 t
ORIGIN      
        1 tgagatcgag aggggtaaga gacatggcgc tatcaccaat tgcctccacg gcggtgccgc
       61 cagcgccgaa tacgatgacc ggcccgaaga catcgtctgt cgatattcca aggatcagtt
      121 cataagcgtc cttgcggtgg atcatcggct gcacggtcac gccttctatg atcgcgtcgg
      181 gatgagccgc tttgatctcg ctcagcatcc gccgggtggc tttctccacc tcctccgaag
      241 atgtgatgtc aagacggacg cctccgatat cggacttatg ggtcaggttc ggcgcgagga
      301 ttttcacgac aaccgaggcg tagtcgcgca agagctctgc cgccgcccgg gccgcctctt
      361 ccggcgttgc ggccaccctc gtcggcacgg tcgggatgcc ataggcgctc agcacagcct
      421 tggcctccgg ttccgtcatc atgctgcgcc cctggccaag ggtatgagcg atgagggcgt
      481 cgacggcggc aggatcgaca tgattctctg gcgtgacagg cggcgtccgc atcaactccg
      541 tttgcgccct cgcgtagcgc gcgagctgca tgatgccctc gatggcttcc gtcggggttt
      601 cgaagtcggg gatgttggcc ttcgcgaaaa gcggccgcac ttcctccacc gcgcttttcg
      661 caagccacgt cgcgaccact ggctttttat ggccaaggcg gcgttgcttc tcaacggctt
      721 cgatcgttgc tttcgccgcg tcgaccgacg aggcaagcgc cgtcgggcag tttataacga
      781 gcacgacatc ggtattggga tcctgcatga cggcttccat ggccgccgcg taacggtccg
      841 gcccagcgtc gccgataatg tcgacggggt tgccatgcga ccatgttggg ggaagcgtct
      901 tattcagggc gtcgatcgtc gccggctcaa gatcggcaag cgtgcctctc aggtcggcga
      961 ccgtgtcggc ggcaacgacg cctgctccgc ctccgttcgt cacgatggca atgcggcttc
     1021 ccggaatgca ccggacacgc gcaagcacct cacccgcatc gaacatctcg tccagatcaa
     1081 caacgcgcag aatgccagca cgccggagcg cagcggtgta gacgttatcg gagcccgcaa
     1141 gcgcccccgt gtgtgaaagt gcggccttcg ctccttccgg gtgccggccg gccttgatgg
     1201 cgatcacagg cttcgcgcgc gagcagcgcc tcgccgcgga catgaacttg gccgcgtcct
     1261 tcacggactc gatataaagg aacacggctc tgtttgtgat gtcgcctgcg agatagtcga
     1321 gcatgtcccc gaggtcgacg tcggcgcaat caccgattga gaccacatgg ctgaagccga
     1381 ccccttcgac ggcggcccaa tcgagcatcg ccagcgtgat ggcgcccgat tgcgacacga
     1441 gcgcgatgtc ccctggcttt gccagcgtat gcgcgaagct cgcgtcgagc ttcaggctcg
     1501 ggatctggat gcccatgcag ttcgggccga tgatgcgcaa gagaaaaggc ttcgccgcag
     1561 cctgcatgcg ctgtgccaga tcacgcgtga tgcccgccgt gatgacgaca gccgctttgc
     1621 agcctttgcg gccaagctcg tcaatgatgc cggggaccgt gttcggaggc gtggcaataa
     1681 cggcgaggcc cggtacgaag gggagggatt caatgctgcg gtgacaaggc ttgcccagaa
     1741 tagcgtcgtg gcctggattg acgaaaccga tttcgcccgc aaagccgccg cgcagaaggt
     1801 tttcggcaac atgcaacccg atggaaccgg gccgctcgct cgcgcccaca agagcgacgc
     1861 tgcgaggctg aaacaggaga ttgagatttc ggatggtcat gtcgcgctcc ggcggccgcg
     1921 agtttggctg tcgcgctccg gcgataggcc cagcgcctcg gccttatctc atattgtcgc
     1981 cgcgaacgta agcgctgaca gcggtcaaaa aaacaatgca acccggaatc gcgccaggcc
     2041 gcaggattgc acaccggcaa ccttataatt gtacgtggtt cacaacccag gcacccgcgg
     2101 cggaagcgag agccgcgaac aagaggggcc acactgtctt ggcgaaaacc ctcagaagcg
     2161 tcacaaaagc ggagaaggct tttgctcccg cgctatagag ttcgcgggcg gcgcgataga
     2221 ggaaataggc aacgatcccg gcgctcgcga tcagcaacaa gaccttcgag ccgggcaact
     2281 cgccgatccg gccccagggc acggaaagca cctgcccaag ccaccacagc caagcttcac
     2341 gcaaataacc gaaaatggcc gcgaatagag catacagcgg cggctgcact tgatccatgt
     2401 cttgaccttc atttgcccaa ggcaattcgt tttgtgctat tatccgccta aaaacatgcc
     2461 gcgagcttgc gggacgcaga gcctgtgcgt aatcatcggg tgactttccg cgttgaggag
     2521 ttgccgttct cgctacggcg ggcgatgcct ggaacctgtc agtgtctctg cggttcaccc
     2581 cggtaggacc ggagattgac ctgcggcata aatcacggaa gcgcgtagaa aaaatatacg
     2641 cgattacact tcccaaaaag caggtagagg cgttaccatc aaagaatgcc aagacgactt
     2701 ttaaatattg gccctttgaa aaccgatggt taccttgacg gtcagctgct tattgccatg
     2761 ccgtcgatga cagattcccg attccatcgg gcggtcatct atatgtgtgc tcactcagcc
     2821 gaaggcgcga tgggattggt tatcaatcag cgggccccta atatcacctt gccgaggctt
     2881 ttggaacagc tcgagatcat cccagaagga cgccgctcaa aagcaatccg cgtgagttcc
     2941 atggctgttc acatgggagg cccggtggaa acaagccggg gcttcgtctt gcatagtgca
     3001 gactattttg ctgcggagag cacactgcca atcaacgaag gcgtttgcct gaccgcgacg
     3061 atcgatattc tcaaggcgat cgcaaaagga agcgggccga acaaagcaat actcgcgctt
     3121 ggctatgcgg gctgggctcc tggtcagctt gaacacgaaa ttcagacgaa tggctggctc
     3181 aattgcccgg ccgatcccga cattattttc aatagcgacc ttgaaagcaa gtatgggcgc
     3241 gctctcgaca gccttggcat cgatccctcg catctcgtcg gggttgcggg cagggcttag
     3301 ctagttcgcg tccataaacg gctgtttccg
//
LOCUS       DATMGX010000893         2067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_203805, whole genome shotgun sequence.
ACCESSION   DATMGX010000893 DATMGX010000000
VERSION     DATMGX010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203805"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1741
                     /locus_tag="VNR65_14650"
     CDS             <1..1741
                     /locus_tag="VNR65_14650"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWJ19958.1"
                     /translation="GSDDPSRYLNDIKTLAAPNMEGRGAGTKGLRRATKYIEHGYKSL
                     GLQPAGTNGYLQPFTVTTGAKLKSDNQFIVDEGDKKRPLPLNQDFVPVSFSSSGSLTA
                     PVVFAGYGASAEEFGYDDYAGLDVKDKIVVVLRYEPEIFGIKSGQHGLTQHSQLITKA
                     INARNHGAKALVVINGKLGDGEEDLLMRFGGVSGPQDVGIMLLQVKNAVADSWFKAAG
                     KSLSDVQQQINHSGKPDSFAFPGTLQLSLKVDIEATHARVNNVLAYLPGKTSEYVIIG
                     AHYDHLGRGDSNSLAPSQIGHIHPGADDNASGTAGVLELARLFAPMKGRLDRGILFMS
                     FSGEELGLLGSAEWVKDPTLPLEKCVAMLNMDMIGRIKDNKVYIGGVGTGSTFKTILE
                     QGQKQKQEPNFKIEYSAGGYSASDHTSFVAKKIPVLFFFSGLHSDYHKPSDTWDKINA
                     PSAAQLLNLVGDVALQLAEAPSRPTFITVVEDKPVSGGGGGYGPYFGSIPDFGEVKDG
                     VRFSDVRPGSPAAKAGLKAGDILVQFGDKPIHNLYDFTDALRRSKVGGVVAVKVLRDG
                     QPITASVKLEQRK"
     gene            1835..>2067
                     /locus_tag="VNR65_14655"
     CDS             1835..>2067
                     /locus_tag="VNR65_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWJ19959.1"
                     /translation="MRNDPALSALFPATRRSLLAATIVQPDKWWYLSELAQHLRTTPS
                     SLQRELSSLVAAGLLQKRREGTRTYFKAETRSPL"
BASE COUNT          443 a          589 c          642 g          393 t
ORIGIN      
        1 cgggtccgac gatcccagcc gctatctcaa cgacattaag acgctggccg cgcccaacat
       61 ggaaggacgc ggcgccggaa ccaagggcct gcgacgggcc acgaaataca tcgagcacgg
      121 ttacaagagc ctgggacttc agcccgcggg cacgaacgga tatctgcaac cgttcaccgt
      181 taccaccggc gcgaagttaa agtcggacaa ccagttcatc gtggacgaag gcgacaagaa
      241 acgcccgctg ccgttgaacc aggatttcgt gccggtcagt ttctcctcat cagggtcgct
      301 gaccgctcca gtcgtctttg ccggttatgg cgcgtccgcc gaagaatttg gatacgacga
      361 ctacgccgga ctggatgtga aggacaagat cgtggtggtg ctgcgctacg agccggaaat
      421 cttcggcatc aagagcggcc agcacggatt gacgcaacat tcgcagttga ttaccaaggc
      481 gatcaacgct cgcaaccacg gagccaaggc gctggtggtg atcaacggaa agctgggtga
      541 cggtgaagag gacctactga tgcgctttgg cggcgtgagc ggaccacagg acgtcggcat
      601 catgctgctt caggtgaaga acgcagtcgc cgatagctgg ttcaaggcag ccggcaaatc
      661 actttccgac gtccagcagc agatcaacca cagcggcaag ccggactcgt ttgcgtttcc
      721 cggaacactg caactgtcgc tgaaggtgga tattgaggcc acgcacgcgc gggtcaataa
      781 cgtgctcgca tatcttccgg gcaagacaag cgaatacgtc atcattggcg cgcattacga
      841 tcaccttgga cgcggcgatt ccaattcgct ggcgccgtcg cagattgggc atatccatcc
      901 tggggccgac gacaacgcct ccggaaccgc gggcgtgctg gaactcgcgc gtttgttcgc
      961 tcccatgaaa gggcggctcg accgcggcat tctgttcatg tcgttctccg gagaagagct
     1021 tgggctgctg ggctcggcgg agtgggtgaa ggacccgacg cttccgctcg agaagtgcgt
     1081 cgccatgctg aacatggaca tgatcggccg catcaaggac aacaaggttt atatcggcgg
     1141 cgtggggacg ggctcgacgt tcaaaacgat tctcgaacag gggcagaagc agaagcagga
     1201 accaaacttc aagatcgaat actccgcggg cggatattcc gccagcgatc acacctcgtt
     1261 cgtggcgaag aaaattccgg tgctgttctt cttctccggg ctgcactcgg attatcacaa
     1321 gccctccgac acttgggaca agatcaacgc gccgtcggcg gcgcagttgc tcaacctggt
     1381 cggtgacgtt gcgttgcaat tggcggaggc tccgtcgcgg ccaaccttca tcaccgttgt
     1441 ggaagataag cccgtgagcg gcggtggcgg aggctatggg ccgtacttcg ggtccattcc
     1501 cgacttcggc gaggtgaagg acggcgtgcg cttctccgac gtacggccgg gatcgccggc
     1561 agcgaaggct gggctcaagg cgggcgacat cctggtgcag ttcggcgata agcccattca
     1621 caatttgtac gacttcacgg atgcgctgcg ccgcagtaag gtgggcggag tcgtggcagt
     1681 gaaagtcttg cgcgatggcc agcccatcac cgcaagcgtg aagttggagc agcgaaagta
     1741 gtatggcgaa cggcgcgatt gagagggtcc aggtccgcag gaacggaagg cttggtttga
     1801 cttgttacgc ataatgcgta acaatttacg cattatgcgt aatgacccgg cactctcagc
     1861 tttgtttcct gcgacccgca ggtcattgct ggccgcgacc attgtgcagc cggacaagtg
     1921 gtggtaccta tccgaactgg cgcagcatct gcgtaccacg ccgtccagtt tgcaacggga
     1981 gctctcctct ttggttgccg ctggcctctt gcagaagcgc cgggaaggaa ccaggaccta
     2041 cttcaaggct gaaacgcgct ccccgct
//
LOCUS       DATMGX010000894         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_433454, whole genome shotgun sequence.
ACCESSION   DATMGX010000894 DATMGX010000000
VERSION     DATMGX010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433454"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VNR65_14660"
     CDS             complement(<1..235)
                     /locus_tag="VNR65_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19960.1"
                     /translation="MFEALSLSISDPATISLAAAMAFTFGAVFFFVVGANAYIRTRNT
                     VRRRSILDQGLASAELATSQGWFENTNSLRYQSI"
     gene            complement(235..1194)
                     /locus_tag="VNR65_14665"
     CDS             complement(235..1194)
                     /locus_tag="VNR65_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWJ19961.1"
                     /translation="MLTHNLASFFVFCTILTLVLAMYIGIRGWVAGRGRIRTRLTAQN
                     EKKIASEAELLGIRRSRSLTREGDYATPFVSLNSLILQSGIRFGLPGIIFAGLACSVA
                     AYVITTLAGLGILIQASCAVACGIGLPLIVLRAMREGRLRRFEEQLPEAMDTIVRSLK
                     AGHAISAAISAVSRHMPDPIGNEFRITAAEMSYGLDLETAMVNLHSRVGQADLGLLSL
                     AVSIQSKTGGNLAEILTTMSRVIRDRLKLRRKARALASEARVSAYILSALPVLLFGIL
                     WLISPGYYGEIWGNVYAKYTLTGAAFWMLLGDYIMYRMARIRV"
     gene            complement(1201..>2044)
                     /locus_tag="VNR65_14670"
     CDS             complement(1201..>2044)
                     /locus_tag="VNR65_14670"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWJ19962.1"
                     /translation="GSRINVAVRPVAVDGPLVSIRKFAEKPYDLKKLASFGTLSDRMS
                     DLLAMCVQSRLTILISGGTGSGKTTLLNALSAYIDDSERLITIEDAAELKLQQPHVGR
                     LETRPPNMEGRGEIRQRELVKNALRMRPDRIILGEVRGEEAFDMLQAMNTGHEGSLAT
                     IHANSPRDALGRLSQMVGMAGLPMSENSIRSQIASAMRLIVQLQRFSDGKRRLISITE
                     VSGMEGDVIQIQELFRFVRHGIAPDGQVLGAFVATGVRPFFVEELKVRGFSVPNTFFG
                     PGTE"
BASE COUNT          469 a          568 c          582 g          425 t
ORIGIN      
        1 gaatgctctg atatcgaaga gagtttgtat tctcgaacca tccttggctg gtggcgagtt
       61 cggctgaagc cagcccttga tccaagatcg aacgccgtct gacggtgttc ctggtccgaa
      121 tataggcgtt agctccgaca acaaagaaga aaacagcgcc gaatgtgaag gccatggcag
      181 ctgccaagct gatggttgcg ggatcagaaa tcgaaagtga aagcgcctca aacattacac
      241 gcggatcctc gccatgcgat acatgatgta gtcgccaagc agcatccaga aggctgcacc
      301 ggtgagggtg tatttcgcat agacattgcc ccaaatctca ccgtaataac cgggagaaat
      361 cagccaaaga atcccaaaca gaaggacggg gagggcgctt agaatatagg cggatactct
      421 tgcttccgac gccagagcac gagcttttct tcgcagtttt aagcggtcgc ggattacacg
      481 cgacatggtc gtgaggattt ctgccaggtt gccgcctgtc ttcgactgga ttgagactgc
      541 cagcgaaagc aggccaagat cggcttggcc aaccctggaa tgcagattga ccatggccgt
      601 ttcgagatcg aggccatacg acatttcggc agcagttatc ctgaattcat taccgatcgg
      661 atcgggcata tgtcgggaaa ccgcagaaat cgccgccgat attgcgtgtc cagctttgag
      721 gctcctgacg atcgtgtcca tggcttcagg cagttgctct tcaaaccgcc gcagccggcc
      781 ctcgcgcata gctcgcaaga caatgagcgg cagtccaatt ccgcaggcga cggcgcatga
      841 cgcttggatg agaataccga gcccagcaag ggtggtgatg acataggctg cgacggagca
      901 agccagccct gcaaagatga tacccggcaa tccgaatcgt attccagact gaagaataag
      961 actgttcaga gagacgaagg gtgttgcgta gtcgccctcg cgggtcaggc tccggctccg
     1021 tcggataccg agaagctcag cttccgatgc aatctttttc tcattctggg ctgtcagccg
     1081 ggttcggatc cgcccgcggc ctgcgaccca ccccctgatc ccgatataca tcgcaagaac
     1141 caaggtgagg atggtgcaga aaacaaagaa ggaggccagg ttatgggtca acatgctcgt
     1201 tcactccgtt cctggaccaa aaaatgtatt tgggacgcta aatcctctga ccttcaattc
     1261 ctcgacgaag aagggtctta cgcccgtcgc cacaaaggcg cctagaacct gtccgtcggg
     1321 ggcaatgcca tgccggacaa aacggaacag ctcctgaatc tggataacat caccctccat
     1381 tccggagact tcggtgatgc tgataagccg gcgtttgcca tcgctgaagc gctggagctg
     1441 aacaatcagc ctcatagctg atgcaatctg agagcggatg ctgttttcag acattggaag
     1501 acctgccatg cccaccatct gggacaggcg gcccagcgca tcgcgcggac tgttggcatg
     1561 gatggttgcg agcgagcctt cgtggccggt gttcatcgct tgaagcatgt cgaacgcttc
     1621 ctcgccgcgc acctcgccca gaatgatacg gtcggggcgc atgcgaagcg cattcttgac
     1681 gagttcgcgc tgacggattt cgccgcggcc ttccatgttg ggaggccgtg tttcgagccg
     1741 gcccacatgc ggctgctgca gtttcagttc ggcggcgtcc tctatggtga tgagccgctc
     1801 cgaatcgtcg atgtaagctg agagcgcatt caagagcgtc gtttttccgc tgccggtgcc
     1861 tcccgagatc agaatggtca gccgcgactg cacgcacatc gcgagcaggt ccgacatcct
     1921 gtcgctgagt gtcccgaaac tggccagttt cttcagatcg taaggtttct cggcgaattt
     1981 tctgatggaa accagtggtc cgtccaccgc gacgggacga accgccacgt tgatacgaga
     2041 cccg
//
LOCUS       DATMGX010000895         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_26098, whole genome shotgun sequence.
ACCESSION   DATMGX010000895 DATMGX010000000
VERSION     DATMGX010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26098"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..1711
                     /locus_tag="VNR65_14675"
     CDS             392..1711
                     /locus_tag="VNR65_14675"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWJ19963.1"
                     /translation="MKNSICSIVVLLLSLSAIGQSNPAQIPQSPQVTVIRAGTLIDPR
                     AAEPKRNQLIVIRGDKIEAVGDAASTQVPVGAKVIDMSNATVLPGLIESHTHIFLQGE
                     DPALGGYDIQLLKFPLAYRAARATVSARRALEQGFTTIRDMETEGAGYGDVGIKMAVE
                     QGYIPGPRIFTTTRGISTTGGYPLEGYAPEIQVPKGVQIIDGPVEARKAAREQMEHGA
                     DWIKVYMTHRSWADKDGHLVSQPTLTVEELKAIVDETHSWGRKVACHAYNGEGLHRAL
                     DGGCDSIEHGLDLDDAAIAQMKRQGTWYCPTIAIYYKEWSPENTPAGQRDRARAADHE
                     QSFKKAFQAGIKIVFGTDVGGFSWTEPIAQEFPYMVKFGMSPIEAIRSATISPAEMLN
                     MSGQIGVIAPGAYADVIAVAGDPLKDITELGRVKFVMHNGEVFESSF"
     gene            complement(1853..>2585)
                     /locus_tag="VNR65_14680"
     CDS             complement(1853..>2585)
                     /locus_tag="VNR65_14680"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HWJ19964.1"
                     /translation="FFRYLVRVLVLIVVFMASALTAMRFAIHGRQTTIPKLVGMAPLQ
                     AERLLADHGLVLERSDRFFSSDVPEGRIMSQAPLPGVQVRRGWRVRVAESMGPQRVVI
                     PNLIGGSERAAEINIRRRGLELGSIATATIPGGPTDQIVAQSPPANAVNVSAPKISVL
                     LAAPEERNSFVMPDFVGRGEDDAINAIVGAGFHVGGIASQPLPASDSTSEEAAALPSR
                     VVVRTTPAAGQRIYEGQGIGLEVTP"
BASE COUNT          532 a          773 c          796 g          484 t
ORIGIN      
        1 tttttcgtga catccgcccg gcggcgacaa tggtcgaagt atcgaagctg atctcgccgg
       61 aaatgctggt cgagatcgaa gtggacgcga tcatcgcgtc ggagatcgga tagtgcggcg
      121 cttcacctgc attattcaag agcgcaagta gttttttgat tgtcatcctg agcgacgagc
      181 gcagcgagga gtcgaaggaa ccctttggcc ggcggcaatc tcaaaacttg ttgctgcgcc
      241 acgcggccac attgtgattt ctcgcagcca aagggatcct tcggctctgc gcccgcccga
      301 cccgcaaaag aagcgggtcg gaaagccttt gcgggcgctg cgctcaggat gacactgagt
      361 tccggcaatt agcctttagt agaggagcac aatgaagaat tccatctgta gcatcgttgt
      421 cctgctgctc tcattgtccg caatcggcca atcgaatcct gctcaaatcc ctcaatcgcc
      481 gcaggtcacg gtgattcgcg cgggaacgct catcgatcca cgcgctgccg aaccgaagcg
      541 caaccagttg atcgtgattc gtggcgacaa gatcgaggcc gtcggcgacg ccgcttcgac
      601 gcaagtccct gtcggcgcaa aggtcatcga tatgtcgaac gccaccgtgc tccccgggct
      661 gatcgagtcg cacacccaca tctttctcca gggagaagac ccggcactgg gcggctatga
      721 cattcaactc ctgaagttcc cgctggcgta ccgtgcggca cgggcgacgg tttcggcgcg
      781 gcgcgctctt gagcagggct tcaccaccat ccgcgacatg gagaccgagg gcgccggcta
      841 cggcgatgtc ggaatcaaga tggcagtcga acagggttac attcccggac cgcgtatttt
      901 taccaccacg cgcggcattt cgacgaccgg cggctatccg ctggaaggtt atgcgccgga
      961 gatccaggtc cccaaagggg tccagatcat cgacggtccg gtggaggcgc gtaaggcggc
     1021 gcgcgaacaa atggagcacg gtgcggactg gatcaaggtg tacatgacgc atcggtcgtg
     1081 ggccgacaaa gacggccact tggtttcgca acccacgctg acagtggaag aactgaaggc
     1141 catcgtggac gagacgcaca gttggggccg caaggtcgcc tgccacgctt acaacggcga
     1201 gggtctgcac cgcgcgctgg acggcggatg cgactcaatc gagcacgggc tcgacctcga
     1261 cgatgctgct atcgcacaga tgaagcggca aggcacgtgg tactgcccga ccatcgcgat
     1321 ttactacaag gagtggtcac cggagaacac gcccgcaggc caacgcgacc gcgcccgagc
     1381 cgctgaccat gaacagtcct tcaagaaggc gtttcaggct gggatcaaga tcgtcttcgg
     1441 cacggatgtg ggcggcttct cgtggactga gccgatcgcc caggaatttc cttacatggt
     1501 gaagttcggc atgtcgccaa tcgaggcaat ccgctcggca acgatcagtc cggcagagat
     1561 gctcaatatg tcgggacaga ttggcgtgat cgcgcccgga gcctacgccg atgtaattgc
     1621 agtggcgggc gatccgctga aggacatcac ggaattggga cgagtgaagt tcgtgatgca
     1681 caacggggag gtgtttgaga gcagctttta gctcttagct cttagcattc tgggcattct
     1741 cttgcctttg ctagtgggcc gtttcaacat tcgaaatggt aggttccttg cagttcgcgt
     1801 ttgctaaaag ctaagagcta agagctaaga gctaacggct aagagctgtc cgctacggcg
     1861 tcacttccag cccaatcccc tggccctcat atatcctctg gcccgctgcc ggcgtggtgc
     1921 gcacaacaac gcggctaggg agagcagcag cctcctcgga agtgctatcg ctggcgggaa
     1981 gcggttggct cgcgataccc cccacgtgaa acccggcccc gacgatcgcg ttaatcgcgt
     2041 cgtcttcgcc gcgcccgacg aagtccggca tgacaaaaga gttgcgctcc tccggcgcgg
     2101 ccagcaggac gctgatcttc ggcgcggata cgtttaccgc gttggcaggc ggactctgcg
     2161 ccacgatctg atcggtcggg ccacccggaa tggttgcggt tgcgatgctt ccaagctcca
     2221 ggccgcggcg gcggatattg atctcggcgg cccgctcgct gcctccgata aggttgggga
     2281 tgaccacgcg ctggggtccc atgctttcgg cgacgcgtac acgccaacct cgccgcacct
     2341 gtacccccgg cagaggcgct tgtgacatga tccggccttc cggaacgtcg gagctgaaga
     2401 agcggtcact gcgttccagc accagcccat ggtcagcgag caaccgctcc gcctgaagag
     2461 gagccatccc aaccagtttg gggatggtgg tctggcggcc gtggatggca aagcgcatcg
     2521 ccgtcagcgc cgacgccatg aacactacga tcagcaccag cacgcgcacc agatagcgga
     2581 agaag
//
LOCUS       DATMGX010000896         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_338448, whole genome shotgun sequence.
ACCESSION   DATMGX010000896 DATMGX010000000
VERSION     DATMGX010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338448"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1148)
                     /locus_tag="VNR65_14685"
     CDS             complement(198..1148)
                     /locus_tag="VNR65_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ19965.1"
                     /translation="MSRHAKRKSSKLKRPKTKLGLPDLDHSKSAVLDSLRSPESKRGY
                     RHAIDEFIQWYCSEPRLSFNKVVVTRYRIFLENRRLAPGTINGRLAAVRRLAYEAADA
                     GLLSPELAAGIRRVKGARKLGVRLGNWLTTDEARKLWQAPDPDTLKGKRDRAILAILL
                     GCGLRRRELADLEFTHIQQREDHWAIVDLVGKGGHIRTVPMPGWVKAAVDLWVAAAGI
                     SAGRLFRCVCRAGKHWGDGVTERVVWHVVKQYAKILGFARLAPHDLRRSCAKLCHSAG
                     GELEQIQFLLGHVSVQATERYLGCKQRIQGAVNDHLGIAP"
     gene            1357..2241
                     /locus_tag="VNR65_14690"
     CDS             1357..2241
                     /locus_tag="VNR65_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWJ19966.1"
                     /translation="MTQLRQLMLEELRRRNFADTTIRSYLHGVEHFSRYFRRRPDQLG
                     PEDIRKYQAMLFTQLKFSPNTVILRLASLRFFYIHVLKRGWSIAETPYPKKVRHLPQV
                     LSQEEVARLIDAAESPFHRILLMTLYATGARRAEAAHLKIGDIDSQRMVVHIRGGKGG
                     LDRDVMLSPKLLEALRTYWRGLRRKPTEWLFPGNRWHTASYPVTTKVLWTACQKAAER
                     AGLAHKHIHPHTLRHCFATHLLEAGADLRTIQILLGHRDLEVTTVYLHLSQRHLSATA
                     SPLDALTFSAPGGHKHRS"
     gene            2238..>2637
                     /locus_tag="VNR65_14695"
     CDS             2238..>2637
                     /locus_tag="VNR65_14695"
                     /inference="COORDINATES: protein motif:HMM:NF025677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HWJ19967.1"
                     /translation="MSRPPLEVADIVRCAGQAFLEHSRKWINWQHQKVLLAITRCRTA
                     ALGGHRDQCSDCGHTAISYNSCRNRHCPRCQGNARLRWLQAREQELLPTRYVHAVFTL
                     PRELAPLALQNKRLIYNLLFHASAETLLGIA"
BASE COUNT          501 a          856 c          744 g          536 t
ORIGIN      
        1 tctgactgcc ttcttccgtg gcctaggctg aggccatgct tacgatccgc acttccagat
       61 gcaacaccat aatttaagaa gccatcctca gctctttttc tttggcttca caattcaaga
      121 ggctggtttg tcggctactc ggaagtaatc cagtttgacg ggttgtcggc gtcgcgctcc
      181 tgtccgaagc agccgcatta tggtgcgatg ccgaggtgat cattcacggc cccttgaatc
      241 cgctgcttgc agccgagata ccgttcggtt gcctgcacgg aaacgtggcc caaaaggaac
      301 tgaatttgct ccaattcgcc gcctgcggaa tggcacagct ttgcgcacga gcggcgaaga
      361 tcatgtgggg cgagccgcgc aaaaccgagt atcttcgcgt actgtttcac cacgtgccac
      421 accacccgct ccgttactcc atcaccccaa tgcttgcctg ctcgacagac gcatcgaaac
      481 aaccgtcctg ccgaaatccc ggccgccgcg acccacagat ccacggccgc cttgacccag
      541 ccgggcatcg gtacggtccg aatgtgtccg cctttgccca ccagatcgac gattgcccaa
      601 tggtcctcgc gctgctgtat atgcgtgaac tccaggtcag ccaattcgcg gcgcctcaga
      661 ccgcagccaa gcaggatggc gagaattgcc cggtcccgct tgcctttgag cgtgtcaggg
      721 tcaggcgcct gccagagttt gcgggcctcg tcagtggtga gccaatttcc aagccggaca
      781 cccaacttcc tggctccttt cacccgccgg atgccggcgg cgagctccgg actgagcaag
      841 ccagcgtcgg cagcctcgta ggcgagccgc cgcactgcag cgagtcgtcc attgatcgtt
      901 cccggcgcga gccggcgatt ctcgaggaaa atgcgataac gggtgaccac caccttgttg
      961 aacgacaagc gtggctcgga gcagtaccac tgaatgaatt catcgatcgc gtgtcgatat
     1021 ccgcgcttcg actcgggcga gcgaaggcta tcgaggaccg ccgatttgct gtggtccaga
     1081 tctgggaggc cgagctttgt tttcggccgc tttagtttcg aggatttccg cttggcgtgt
     1141 ctgctcattc cagccgcctc caccgcggct ggccaagagc ctatgcctgc acctagacgc
     1201 gtcgggcgtt ttacgtccaa gaggcggtgc caatgcggtt gccggatgct ggccgacttg
     1261 aacgaagccg ctttgcggcg gacgcattcg cgtgttgttg agccggcagt ctcgcggtga
     1321 tgtgagctag tttgtgtcgc agaggaggaa ctctctgtga cccaactacg tcagctcatg
     1381 cttgaggagt tgcggcgccg caacttcgcc gatacaacca tccgctcgta cctccacggg
     1441 gtggagcact tcagccggta cttccgccgc cggcccgatc agcttggccc tgaggacatt
     1501 cgcaagtatc aggcgatgct cttcacccag ttgaagttca gtcccaacac tgtgatcctg
     1561 cggctggctt cgttgcgctt cttctatatt cacgtgttga agcgcggctg gagcatcgcc
     1621 gagacgccat atccgaagaa ggttcgccac cttccgcagg tgctcagcca ggaggaggtt
     1681 gcgcgtctga ttgacgccgc agaaagcccc ttccaccgca tcctgctaat gacgctgtat
     1741 gccaccgggg cacgtcgcgc ggaagcagcg catctgaaga tcggcgacat tgacagtcag
     1801 cgcatggttg tccatatccg gggaggcaag ggaggcttgg atcgagacgt catgctgagc
     1861 ccgaagcttc tggaagcgct gcgcacttac tggcgcgggc tccgacgcaa gccgaccgag
     1921 tggctgtttc ccggcaaccg gtggcatact gcaagttatc ccgtcaccac caaggtcctc
     1981 tggaccgcct gtcagaaggc cgctgagcgc gccggcttgg cgcacaagca catccatccg
     2041 cacacgctac gccattgctt cgccacccac ctgctggaag ccggagccga cctgcgcacc
     2101 atccagatac tgctcggtca tcgcgacctc gaagtaacta ccgtctatct gcatctctcc
     2161 caacggcatc tcagcgcaac cgccagcccg ctcgatgccc tcaccttctc tgcaccagga
     2221 ggacacaagc accgttcatg agccggccgc cgctggaggt ggccgacatc gttcgctgtg
     2281 ctggacaagc atttcttgaa cacagtcgca agtggatcaa ctggcaacac caaaaagtgt
     2341 tgctggccat cacgcgctgc cgcaccgccg cgctgggcgg acatcgcgat cagtgctcgg
     2401 actgcggaca tactgccatc tcgtataact cgtgcagaaa ccgccattgc ccgcgctgcc
     2461 agggcaacgc gcgcctgcgc tggctccagg cgcgggaaca ggaactgctg cccactcgct
     2521 atgtacacgc cgtcttcacc ttgccgcggg aactagcccc gctggcattg cagaacaagc
     2581 ggctgattta caacctgctc tttcatgcca gcgccgagac cttgcttggg atcgctc
//
LOCUS       DATMGX010000897         2950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_387890, whole genome shotgun sequence.
ACCESSION   DATMGX010000897 DATMGX010000000
VERSION     DATMGX010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387890"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1035)
                     /gene="nifH"
                     /locus_tag="VNR65_14700"
     CDS             complement(142..1035)
                     /gene="nifH"
                     /locus_tag="VNR65_14700"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HWJ19968.1"
                     /translation="MALRQIAFYGKGGIGKSTTSQNTLAALAEMGNKILIVGCDPKAD
                     STRLILHAKAQDTILSLAAEQGSVEDLELEDVMKIGYRGIKCTESGGPEPGVGCAGRG
                     VITAINFLEENGAYEGVDYVSYDVLGDVVCGSFAMPIRENKAQEIYIVMSGEMMAMYA
                     ANNIAKGILKYANSGGVRLGGLVCNERQTDKELELAENLSAKLGTKLIYFVPRDNIVQ
                     HAELRRMTVIEYAPDSAQAQHYRNLATKVHANIGQGTIPTPITMDELEDMLMEHGILK
                     AVDESVVGKTAAELAAEAAAA"
     gene            1599..1985
                     /locus_tag="VNR65_14705"
     CDS             1599..1985
                     /locus_tag="VNR65_14705"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HWJ19969.1"
                     /translation="MTYFSKIGGAPAVDKIVEEFFRRMETLPEARTVRELHADDLAKT
                     KSVMKRYFSELLGGPMQFSESRGHPALKGRHAGMPIGPQERDAWMRCMKEALAKVVPD
                     ESLRLHIEERFYQLADWVRNDEEKPK"
     gene            1982..2572
                     /locus_tag="VNR65_14710"
     CDS             1982..2572
                     /locus_tag="VNR65_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19970.1"
                     /translation="MTKITDTAFARLEAEGRLVNPVLKAPTRKAGRFGFRGDVALKFA
                     PQFADEARPPELKAEQVMAIATEGVPTIPFFTAYLHSFEYLKTLADVLGETLSPTGKY
                     FVYCNNIDLSKKYSVPYNGARFYVLPIDEAGVYNEVLELLYLEKTALKKLDTAGKLDA
                     VADAALNFEESFPEITYEEGLKLMGPVRNKNENRPV"
     gene            2643..>2950
                     /locus_tag="VNR65_14715"
     CDS             2643..>2950
                     /locus_tag="VNR65_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19971.1"
                     /translation="MNTPHRRPAPRSFRSAFAVILGTNEIASAVAVYMHRAGWCTLLS
                     HDPFPPVIRRRMAFHDALFGEKADVDGLPGVSAETTFEAATLLGGGGCVVVTRLGLLD
                     L"
BASE COUNT          567 a          874 c          860 g          649 t
ORIGIN      
        1 tgccttgata tcttcaacgt tttcttcaac ttccaggctc atgccgctag cctttccaac
       61 ctgagtttct tgcttccggt gccggctctt cgctttgaag agccggtcgg atgcggatca
      121 atggcctgtc cggcctacgt actatgccgc tgctgcttct gctgccaatt cggcagcggt
      181 cttgccaacg acgctctcgt cgaccgcttt caggatgccg tgctccatca gcatgtcttc
      241 gagttcgtcc atcgtgatcg gggtcgggat ggtgccctgg ccgatattgg catgaacctt
      301 cgtcgcaaga ttgcggtaat gctgggcttg cgcggagtcc ggtgcatatt cgatcaccgt
      361 catgcggcgc agctcagcgt gctgcacgat gttgtcgcgc ggcacgaagt agatgagctt
      421 ggtgccgagc ttggcggaga ggttttccgc gagctcgagc tccttgtcgg tctggcgctc
      481 attgcagacc aggccgccca agcgcacgcc gccggagttg gcgtatttga gaatgccctt
      541 cgcgatgttg ttggccgcgt acatggccat catctcgccg gacatcacga tgtagatttc
      601 ctgcgccttg ttctcgcgaa tgggcatcgc gaagctgccg cacaccacgt cgccgagcac
      661 gtcgtaggag acgtagtcga caccctcata agcgccgttc tcttcgagga agttgatggc
      721 ggtgataacg ccgcggcctg cgcaaccaac tcccggctcg ggtccacccg actccgtgca
      781 tttgatgccc cgatagccga tcttcatcac gtcttcgagt tcgaggtcct cgacgcttcc
      841 ctgctcggct gcgaggctga ggatggtgtc ttgggccttg gcgtgaagaa taaggcgggt
      901 cgagtctgcc tttggatcgc agccgacgat gaggatcttg ttccccatct cggcgagcgc
      961 cgcgagcgta ttctgggagg tggtcgactt accgataccc ccctttccgt agaatgcgat
     1021 ctggcgtaat gccatgcgtg tctccttcgt cgtcgtaact cgcgaccggc cggcaaggcc
     1081 gagcgcactc cttagttcgc cagtgatcga aacgcccaac gagcgccgtc gctatcgttt
     1141 gcggggcttc tgctcatcct cggagggcgg agcatctggc aattccttgc ccgcagcccc
     1201 ctttcagcaa gcgccgtgcc aacacgacaa actgctgttt tgacgtggtt ttttaatttt
     1261 ctggcaaaaa ctgtggcatc aggcgtgttt caaagccgac atgcgctcat ggcttgtatg
     1321 aaagtgaaca gcccctcgtc cccctgtggt attttgaagg ccgtgcgcca agggcagaag
     1381 ttcggatcaa tcgtcgacaa aaactttgag cctatagaga acatagcgcc agagaagaag
     1441 tcggacgctg tgtgaagtcc gacaggagct gtcgccattg cgacaaactc agcccttttg
     1501 ccgcgtccgt cttagcaaaa cttgcgtcaa agctcaggtt gcgccatttg gtacggaggt
     1561 tgctagtaac cccctcaatg gctgaaaaga gctctacaat gacatatttc tcgaagatcg
     1621 gcggcgcgcc cgccgtcgac aaaatcgtgg aagaattttt tcgccgcatg gagactttgc
     1681 cggaagcccg aactgtccgc gaactgcatg ccgacgatct cgccaagaca aaatctgtca
     1741 tgaagcgcta cttcagcgaa ttgctcggcg ggcccatgca gttctctgaa tcgcgtggcc
     1801 atcccgcttt gaaggggcgc catgccggca tgcccattgg gccgcaggag cgtgacgcgt
     1861 ggatgcggtg catgaaagag gccctcgcaa aggtcgtacc cgacgagagc cttcgcctgc
     1921 acatcgaaga acgcttttac caactcgcgg attgggtccg caacgatgag gaaaaaccaa
     1981 aatgacaaaa ataaccgaca cggcgttcgc gcggcttgag gcggaaggcc gccttgtcaa
     2041 ccctgtgctg aaagcgccga cgcgcaaggc tggccgcttc gggtttcggg gcgacgtcgc
     2101 cttgaaattc gcgccgcagt tcgccgatga ggcgcgcccc ccggagctga aagccgagca
     2161 ggtgatggcg atcgccacgg aaggcgtccc tacgatcccg tttttcacgg cctatttgca
     2221 ttctttcgaa tacttaaaaa cccttgccga tgtgctgggc gagacgctgt cgccgacggg
     2281 caagtatttt gtttattgca acaacatcga tctctcgaaa aagtatagcg tgccttacaa
     2341 cggtgctcgc ttctacgtgc tgccaatcga cgaagccggg gtctataacg aggtcctcga
     2401 gcttctgtac ctggaaaaga ccgcattgaa gaagctcgac acggcgggga agctcgacgc
     2461 agtggccgac gccgccctga acttcgagga aagctttccc gagatcacct atgaggaggg
     2521 gctgaaactc atggggccgg tgcgcaacaa gaacgaaaac aggcccgtgt aggtcgccca
     2581 cacaagctgc tcgagagcgc ccggtcatgc gccggagtct cttcagctgc gggaggtacc
     2641 ccttgaacac gccacaccgg cggcctgcgc cgcgcagctt tcgatctgcg tttgccgtca
     2701 ttctcgggac aaacgagatt gcttcggcgg tcgcggtcta catgcatcgc gcgggctggt
     2761 gcacgctcct ctcgcatgac ccctttccgc cggtcatccg gcgacgcatg gcgtttcacg
     2821 atgccctctt cggggagaag gcggatgtcg atggcctgcc tggcgtaagc gctgaaacga
     2881 cttttgaagc cgccaccctt cttggtggag gcggttgcgt ggtggtcacc cggcttggcc
     2941 ttctcgacct
//
LOCUS       DATMGX010000898         2601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_433675, whole genome shotgun sequence.
ACCESSION   DATMGX010000898 DATMGX010000000
VERSION     DATMGX010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433675"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VNR65_14720"
     CDS             <1..1285
                     /locus_tag="VNR65_14720"
                     /inference="COORDINATES: protein motif:HMM:NF026213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HWJ19972.1"
                     /translation="TGLIAPTRYVEKPIEEFEVDGVRMIFQNTPNTEAPREMNTYIPD
                     MKALWMAENVIASLHNIYTLRGAPVRDPLNWSKYIGEALYRFGLEAEVMFASHHWPRW
                     GNARIQDVLSAQRDLYAHMNNQVLHFANQGVTINEIHNVYEMPKGLQTKWYCRGYHGS
                     PQHNSRGVIQRYLGFWDCNPTSLIPLPQRESAPLYVEMMGGAGKIMARGQELHNEGKY
                     LLASEIVNKLVQAEPQNQAAKDLLADIFEQLGYQQENPGLRNSFLAAAYELRTGIPQG
                     ETANSASPDVVRAMSTELFLNFLGIRMDSRKAEGMRFTINLITPDNGEKFVVELENAT
                     LTNIKGFLAKKPNLTLTINRSDLELTMMGAKTLDAQIADGTAKVDGDVNILKQLASTM
                     VDFDPRFEIMPGTKSRTIVAHEDPYKADPLQPIAE"
     gene            complement(1327..1599)
                     /locus_tag="VNR65_14725"
     CDS             complement(1327..1599)
                     /locus_tag="VNR65_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19973.1"
                     /translation="MATFCDGTAFTHAISDHSKQAFDKPSSEQAAKAKAVINFLMQDR
                     FFLALFRSNDPKVRAAALSELNEAHYQAYGDAPTQDEDACRESLRK"
     gene            complement(1813..2184)
                     /locus_tag="VNR65_14730"
     CDS             complement(1813..2184)
                     /locus_tag="VNR65_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19974.1"
                     /translation="MNKHNAYMAVPEARSSFQVNLLSAFDEIAQTFLQAAQGKVHTSL
                     QRYLDGNVAIGGDCFTRRAILLSIHDMKEDLEAPLALQEKVLAELRSLSVRPAPQLKL
                     YRDIATALLRMEVVIRSVAVH"
BASE COUNT          626 a          742 c          706 g          527 t
ORIGIN      
        1 gacgggcctg attgcgccca cccgctatgt cgaaaaaccc atcgaggagt tcgaggtcga
       61 cggcgtcagg atgatcttcc agaacacgcc gaacactgaa gcgccccggg agatgaacac
      121 ctatatcccg gatatgaagg cgctgtggat ggccgagaac gtcatcgcat cgctgcacaa
      181 catttacacc ttgcgcggcg cgccggtgcg cgacccgctg aactggtcga aatatatcgg
      241 cgaggcgctc tatcgtttcg gtctggaggc cgaggtgatg ttcgcctccc accattggcc
      301 gcgctggggc aatgccagga tccaggacgt tttgagcgcg cagcgcgatc tctacgccca
      361 tatgaacaat caggtgctgc acttcgccaa tcagggcgtc acgatcaacg aaatacacaa
      421 cgtctatgag atgccgaagg gcctgcagac caaatggtac tgccgcggct atcacggctc
      481 gccgcagcac aacagccgcg gcgtcatcca gcgctacctc ggcttctggg attgcaaccc
      541 gacctcgctc attcccttgc cgcagcgaga gtctgcgcct ctgtatgtag agatgatggg
      601 gggcgccggc aaaatcatgg cgcggggaca ggaactgcat aatgagggga agtatctcct
      661 cgcatcggag atcgtgaaca agctggttca ggcggagccg cagaaccagg ccgccaagga
      721 cctgcttgcg gatattttcg agcagctcgg ctaccagcag gaaaatccag gcctccggaa
      781 cagctttctc gccgctgcct atgagctccg taccggaatc ccgcaagggg aaaccgccaa
      841 ttcagcaagc cccgatgtgg ttcgggccat gtccaccgaa ctgtttctca acttcctggg
      901 catccgaatg gacagccgca aagccgaagg aatgcggttc acgatcaacc tgatcacgcc
      961 ggacaacggc gagaaattcg ttgtcgaatt ggaaaacgcc acgctcacga acatcaaggg
     1021 cttcctggcc aagaagccca acctgaccct taccatcaat cggtcggatc tcgaacttac
     1081 tatgatgggg gcgaaaacgc tcgacgcaca gattgcggac ggaaccgcaa aggtggacgg
     1141 cgacgtcaat atcttgaaac agctcgcgtc gacgatggtc gacttcgacc cgcgctttga
     1201 gatcatgccc ggcaccaaat cccgaaccat tgtggcccac gaagatccgt acaaagccga
     1261 tccccttcag ccgatcgcgg aatagggggc gcatatcgag tgtaggccga caacaagagg
     1321 gcgatgctat ttccgcagtg actcgcggca agcatcctca tcttgggtcg gggcatcgcc
     1381 gtaagcctgg tagtgagctt cgttcagctc actcaaggca gcggcgcgca ctttcggatc
     1441 gttgctacga aacagagcaa gaaaaaaccg atcctgcata aggaaattga tgaccgcttt
     1501 ggccttcgca gcttgctctg agctcggctt gtcgaaagcc tgcttggagt ggtctgatat
     1561 ggcatgagta aaagcggtgc catcgcaaaa agtagccatg ttttcctcct ctagagttct
     1621 acttcaattc cgtaccccgc ggcgaaacga ggtcagcgct caactgcctt acgcacgagg
     1681 cggagaatca gccctcaagc ggtccgcccg acgcattcat gtctagatca acccgccttt
     1741 aatcggaaga ttttgggcgg agacgttcat ggatagagca gattatccgc gtccaaaggg
     1801 acgcaggctg ctctaatgca cagcaactga ccttatgacg acttccatcc tgagaagcgc
     1861 cgtggcgatg tcccgataaa gcttcagctg aggggccggc cgcacgctta gcgatcgcag
     1921 ctccgcaaga accttttctt gaagcgcaag cggagcttcc aggtcttcct tcatgtcgtg
     1981 aatagagaga aggatggccc gacgcgtaaa acaatcgccg ccaatcgcga cattgccgtc
     2041 caagtacctt tgcagagagg tgtgaacctt cccttgcgcc gcttggagaa atgtctgcgc
     2101 gatctcgtca aacgcagaaa gcaggtttac ttggaaagag ctccgcgctt caggcacggc
     2161 catgtatgcg ttgtgcttat tcataagggc tcacattttg agcgcaaagc ctcatcgcct
     2221 tgcggattga gatcgaccgc ggccgcacgc gttggcggca cgcctaagca aatgcacacg
     2281 aacttgggtc aatcggaact ggtcaagttc atccgatcga gaaccaagtg gaaactcgct
     2341 caaacaggcc tactgccacc atttgaccga agctaaattt tcagctgatt tggattaaga
     2401 gtagcctaga ctgctagatc cagcaactac gaaaggctga gcggacctaa agaagtgctt
     2461 aacatgctga tacttgcatt aacacttgct attaagtctt tacagatttc gtgcaaaaac
     2521 ccggcaagtt cccttccggt gttgtaattt gaggtcgatt tggaagccgc ttctttagca
     2581 gccgtgcctt ccaacgcatt c
//
LOCUS       DATMGX010000899         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_254591, whole genome shotgun sequence.
ACCESSION   DATMGX010000899 DATMGX010000000
VERSION     DATMGX010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254591"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /gene="egtB"
                     /locus_tag="VNR65_14735"
     CDS             <1..783
                     /gene="egtB"
                     /locus_tag="VNR65_14735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HWJ19975.1"
                     /translation="ENPLCPAYRDAQPAVISGAAAPGHWRSVPGGICEIGHRGAGFAC
                     DNEGPPHQVLLRDFRIFSREVTNGEWIAFMDAGGYRTPLLWLSDGWTTVQREGWEAPG
                     YWDLRDGAWHQMTLHGLRKIEESRPVTHISYYEADAFARWSGHRLPTEFEWEIASRGQ
                     PAPAPIELSYLLPKPASNDGVSSLFGNVWEWTQSAYLPYPGYKPATGALGEYNGKFMC
                     SQMVLRGGSLATPDRHIRPTYRNFFYPHQRWQFMGLRLSSDV"
     gene            783..1739
                     /gene="egtD"
                     /locus_tag="VNR65_14740"
     CDS             783..1739
                     /gene="egtD"
                     /locus_tag="VNR65_14740"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HWJ19976.1"
                     /translation="MGQIVQFPEARVSAGKDFRDAVLMGLSLQQKSVPSRFFYDARGS
                     ELFERITELPEYYPTRTEIRLLKSCGPDIGKLLPSEAVVVEFGSGSSRKTELLLHALE
                     RPTAYIPIEISAAALYPAARRIAKAFPGLGVHPILGGFHDLDGMKLPLQDHFCVGFFP
                     GSTIGNFNRTEAVLFLRSVRRLLGKKAALLVGADLQKPLDVLLPAYNDAAGVTAAFNL
                     NLLSRINRELSGTFDLAAFEHSAIYNDRKERIEMHLRSVCDQRASVAGRSFSFQAGET
                     IHTENSHKYSLSGFRWIAARGGWETVRSWADENRHFSLHLLT"
BASE COUNT          375 a          638 c          574 g          429 t
ORIGIN      
        1 gaaaatccgt tgtgcccggc ttatcgcgac gcgcagccag cggtaataag tggcgctgcc
       61 gcacctggcc attggcgctc agtccctggc ggaatttgcg agatcggtca tcggggcgca
      121 ggttttgcct gcgacaatga aggcccgccg catcaggttc tcctgcgtga tttccgtatt
      181 ttcagccggg aggtcacgaa cggtgaatgg atcgccttta tggacgcggg cggctaccgg
      241 acgccgttgc tatggctgtc ggatggctgg accacggtgc agcgcgaagg gtgggaagca
      301 cccggctatt gggatcttcg cgacggcgca tggcatcaga tgacgctgca tggcctgcgc
      361 aagattgagg aaagccgccc ggttacgcat atcagctatt acgaagcgga cgccttcgcc
      421 cgctggtcag gtcatcgcct tcccaccgag ttcgagtggg aaatcgcctc gcgcggacaa
      481 cccgccccgg cgccaattga gctgtcctat ctcctgccga agcccgcatc caatgacggc
      541 gtttcgtccc ttttcggaaa cgtctgggaa tggacgcaga gcgcctatct accgtatccc
      601 ggctataaac ccgccactgg cgcgcttggc gaatataacg gcaaattcat gtgcagtcag
      661 atggtgttgc gcggcgggtc gctcgccacc ccagaccggc acatccgccc aacctaccgc
      721 aacttcttct atcctcatca gcgctggcag ttcatgggcc tccgcctctc gagtgacgta
      781 taatgggaca aattgttcag tttcctgaag ctcgcgtcag cgcgggaaaa gactttcgcg
      841 acgccgtgct catgggtttg tcgctgcagc aaaagagcgt accgagccgc ttcttctatg
      901 atgcgcgggg ctcggagctt ttcgagcgca tcaccgaatt gccggaatat tatccgaccc
      961 gcactgagat ccgccttctc aaatcctgtg gccccgatat tggcaagctc ctcccgtcgg
     1021 aagcggtcgt tgtcgagttc ggctcgggtt ccagccgaaa gaccgaactc ctgctccacg
     1081 ccctggaacg ccccaccgcc tatatcccga tagaaatctc ggcggcggcg ctttatccgg
     1141 cagcccgtcg catcgcgaag gcctttcccg gccttggcgt gcatcccatt ctcggcggat
     1201 tccacgacct cgacggaatg aagctgccgc tgcaggacca tttctgcgtc ggctttttcc
     1261 ccggatcgac tatcggcaat ttcaatcgca cggaggccgt tctttttctc cgctcggtgc
     1321 gccggcttct cgggaaaaag gccgcgttgc ttgtcggtgc cgacctgcaa aagccactcg
     1381 acgttctgct tcccgcctat aacgacgccg ccggcgtcac ggcggccttc aacctcaacc
     1441 tcttgtcgcg gatcaatcgg gagctctccg gcacctttga cctcgccgct ttcgaacatt
     1501 ccgcgatcta caacgatcgc aaagagcgca tcgagatgca tttgcggtcc gtctgtgatc
     1561 agcgcgcgtc cgtggctggc cgttctttct cgttccaggc gggcgagacg atccacaccg
     1621 agaactccca caaatattcc ctctcgggct ttcgctggat cgcggcacga ggtggatggg
     1681 agacggtaag aagctgggcg gacgagaacc gccatttttc tctgcatctt ctcacgtaag
     1741 agattcggaa gcccatcgac gggctttaac ggcgaggcag atgctcatca gcgacgatgt
     1801 cctgaaagga ccgtaatctg gaatgtcggc gtcctgagct tccgcgcttc gcatacgatc
     1861 ccggacgctc aaggctcacc gtgcgagccg gctgatcgat tgcgtgggcg tgtcctttga
     1921 tgcccgatga caaggccatc gcggaagaag caggcgcctt atgcaaagcc ttgcttgagc
     1981 cgagcgcccc tctctttccc ccaaagaaag ccgcct
//
LOCUS       DATMGX010000900         2201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_51534, whole genome shotgun sequence.
ACCESSION   DATMGX010000900 DATMGX010000000
VERSION     DATMGX010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51534"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..927
                     /gene="dps"
                     /locus_tag="VNR65_14745"
                     /gene_synonym="pexB"
     CDS             439..927
                     /gene="dps"
                     /locus_tag="VNR65_14745"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HWJ19977.1"
                     /translation="MTLHKTRNDLTSNTKTTVAALLNARVADAIDLALMTKQAHWNLR
                     GRQFIAVHEMLDGFRTEVDGHVDTMAERVVQLGGVVLGTTQTVAHCSTLKAYPTDLVS
                     VEDHIAALGERYGDLANATRASIDAADEAGDADTADIFTAFSRSLDKALWFLEAHDAG
                     KA"
     gene            994..1710
                     /locus_tag="VNR65_14750"
     CDS             994..1710
                     /locus_tag="VNR65_14750"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ19978.1"
                     /translation="MALSACTSFPAGGIAVVFGASGGIGSALACRLRAAPCFERVVSF
                     SRSSMPAIDLLTETSLRDAAAFAAGEGDMRLVVDATGFLHDETQRPEKSWRELDPAQM
                     ARSFALNAIGPALIMKHVLPLLPFEGKAVFATLSARVGSIGDNQLGGWYSYRASKAAL
                     NQLVRTASIELTRRQPEAICVALHPGTVATRLSSPFGKAGLDVQSPDVAAERLLAVIA
                     DLGPDDSGGFFDYRGERVPW"
     gene            1814..>2201
                     /locus_tag="VNR65_14755"
     CDS             1814..>2201
                     /locus_tag="VNR65_14755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ19979.1"
                     /translation="MAQDKFHTIVVGGGCLGCASAISVQRRLGGGSEGGHSSVCVIER
                     SVVGSGVTARHSGIVRAANAVPEAAVLAQKAIAQWRNLESLWGVAAPFEACGAIWIAK
                     DAGPGENSSWDALESRMKSAGIEFGRI"
BASE COUNT          406 a          665 c          702 g          428 t
ORIGIN      
        1 cgttattcat gtcatcgacg aggtgctcct gccgaagatg tgagcggagt gtccaacctc
       61 tctctaagac cgtgaaggtg gccgggcgac ggaacatttt cagtccatcg cttggcgtca
      121 tattgtcatt ggtccgccag atcatgatag cttttaagat cgcgacggtg gatacggacc
      181 ggccggcctt gggaagaacg tcgaagccgt tctgccgcgc tcgggccggg cggcccggat
      241 ggatgcaatc ccggcatgat ggcgcttctt ggatgcagct attgactctt cctaggtgcg
      301 caaaccttga tccaggaaag gtttaaggct ggcgcgacaa agacgccggc ctgtcgcaga
      361 ccgaggcgac atctagctcc ggccgcgccc gaccaaacgg ctcggccgcc gtggctcgta
      421 attattagga gttgcccgat gacgcttcac aaaacccgca acgatctgac atccaacacc
      481 aagaccaccg tcgccgcgct tctgaacgca cgcgtggccg acgcaatcga tctcgctttg
      541 atgacgaagc aggcgcattg gaatctgagg ggacgccaat tcatcgccgt gcatgagatg
      601 ctcgacggct tccgaacaga ggttgatggg catgtcgaca ccatggccga gcgcgtggtg
      661 cagcttggtg gggttgtgct cgggacgaca cagacggtcg cgcactgttc cacgctcaag
      721 gcctatccga ccgatctcgt cagcgtggaa gaccatatcg ccgcactggg agaacgctat
      781 ggcgatctcg ccaatgctac ccgtgcgtcg atcgacgctg cggacgaggc tggagacgct
      841 gacacggccg atattttcac ggccttctcg cgttcgctcg acaaagcgct ctggttcctg
      901 gaagcccacg atgcgggcaa ggcttgagat ctaggacata gaggcgcccg gcctttatgg
      961 aggcctggca ggtcatcaac ggcggcggct aacatggccc tcagtgcttg tacctcgttc
     1021 cctgccggag gcattgccgt cgtgttcggt gctagcgggg gcatcggcag cgccctggca
     1081 tgccgcctcc gcgccgctcc gtgctttgag cgggtggtga gcttcagccg gagcagtatg
     1141 ccggcaattg acctcttgac cgagacgagc cttagagacg cggcggcctt tgcggctggc
     1201 gaaggagata tgcgcctcgt tgtcgacgcg accggctttc tccatgatga gactcagcgc
     1261 ccggaaaaga gctggcgcga actcgatcct gcgcaaatgg cgcgttcctt cgcattgaac
     1321 gccatcggcc ccgccctgat catgaaacat gtccttcccc tgctgccgtt cgagggaaag
     1381 gcagttttcg cgaccttgtc ggccagggtg ggcagcatcg gcgacaacca actgggcggc
     1441 tggtacagct accgggcctc caaggccgcg ctcaaccagt tggtgcgcac ggcctcgatc
     1501 gagctcacgc gccggcagcc cgaggcgatc tgcgtggcgc ttcatcccgg cacggtcgcc
     1561 acgcgcctgt cgtccccctt cgggaaggcc gggctcgacg tgcaatcccc ggacgttgcg
     1621 gcagagcgcc tgctcgccgt catcgctgac ctgggcccgg acgattccgg cggctttttc
     1681 gattatcgcg gggagcgggt accatggtga gccagggcgg cgcctggccc aagcgctacc
     1741 acaagtccga ctttcaaagc actaaagggg tgcattctcc agcgtcaacg cgacgtccgt
     1801 cataggaggg atcgtggctc aagacaaatt ccatactatt gtcgtcggtg gcggctgcct
     1861 tggatgcgcg tcggcgatct ctgtacaacg tcgtctcggc ggcggttcgg aggggggaca
     1921 ttcgtcggtc tgcgtgatcg aaagatcagt cgtcgggtct ggcgtcaccg cccgccactc
     1981 cggcattgtg cgagcggcca acgctgtgcc agaggccgca gtgcttgccc aaaaagcgat
     2041 cgctcaatgg cggaatcttg aatcgctttg gggggtcgcg gcgcctttcg aggcctgcgg
     2101 agcgatctgg atcgccaagg acgccggacc aggcgaaaat tcgagttggg acgcgcttga
     2161 atcccgaatg aaatcagccg ggattgaatt tgggcggatc a
//
LOCUS       DATMGX010000901         2298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_50793, whole genome shotgun sequence.
ACCESSION   DATMGX010000901 DATMGX010000000
VERSION     DATMGX010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50793"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VNR65_14760"
     CDS             complement(<1..509)
                     /locus_tag="VNR65_14760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HWJ19980.1"
                     /translation="MSAYDHKIESLGFQGFVNTVFGRVASRYDLMNDLMSGGVHRLWK
                     DAAINWLAPSRSTASFLIDVAGGTGDITRRFLRAAGPGSKSVICDISGEMMEEGERRL
                     ADEHALGHVRFVQANAESLPFKENTSDFYTISFGIRNVPGIQSALNEAFRVLKPGGRF
                     LCLEFSRVEV"
     gene            885..>2298
                     /locus_tag="VNR65_14765"
     CDS             885..>2298
                     /locus_tag="VNR65_14765"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWJ19981.1"
                     /translation="MSFKSSAAPIPQGVAAIPQGRAGALALAALGVVFGDIGTSPLYT
                     LKVVLASSGAQAADRAMALGSLSLIFWTLIIVTSLKYVAVAMRVDNHGEGGIMALMAL
                     VGGRKGLRVRNRKRSIIMAAGLFGAALIYGDGVITPAISVLSALEGLSIVTTSLEAYV
                     VPATVGILVLLFLVQPRGSASIGKMFGPIMAVWFLCLGLLGLWGIVQNPSVIEAVNPA
                     LGLHYLVSGGTTSFLVLGAVFLCVTGAEALYADMGHFGRLPIRWAWTFIVFPCLVLNY
                     AGQSALVLNGAPLSENMFYALCPRPLLVPLVVLATAATIIASQAIITGAFSMTRQAIQ
                     LGWLPRLRITQTSALGYGQIYIGVVNWFMMAITIALTVAFRKSENLAAAYGIAVSATM
                     LMTTVLLYIAMVEKLKWSASASAPIAGSFAIVDSAFLIANSAKIAEGGYVPLALAVVI
                     FSLMWIWHSGRDAIMQVLRSK"
BASE COUNT          435 a          664 c          685 g          514 t
ORIGIN      
        1 acttcgaccc gcgaaaattc aaggcaaaga aagcggccgc ccggcttcag cacgcggaag
       61 gcctcgttga gcgcggactg gatgcccggt acattccgga tgccgaatga gatggtgtaa
      121 aagtccgagg tgttttcctt gaaggggagc gactcggcgt tggcctgcac gaagcggaca
      181 tgcccgagag catgctcatc tgcgaggcgg cgctcgcctt cttccatcat ctcaccgctg
      241 atgtcgcaaa taacggactt ggagccgggg ccagccgcgc gtaggaagcg gcgggttatg
      301 tcgccggtgc cgcccgcaac gtcgatcaag aacgaggccg tagagcgcga gggtgcaagc
      361 cagttgatgg ccgcgtcctt ccagagccgg tgcacgccgc ccgacatcag gtcgttcatg
      421 aggtcatagc gagaggcgac gcggccgaag acggtattga cgaaaccctg aaagccgagg
      481 ctttcgattt tatgatcgta ggctgacatt ccgttcgctc ccgcaaattc ccgcaagttc
      541 tgacgcaccg cttcgctatt gtaaacgcgg cattgtgttt gcgggctcag gaggcgccac
      601 ctgtggcaca gctctcatac tttcgtcggg catagtggtg cactttggtg caatgcggca
      661 agaagtgtct tgcggaattg gtaaaggaat gaaagtaaag cgcacttaga ccgcttttcg
      721 cagatgcgaa gcgcagaggc gccacttgcg aacaggaata ataagaccgt tctgcacgtt
      781 cgttctgtca gcgcccataa aaccaacaaa gcaccttaat gcacctgtta cgatcccatg
      841 ctagagctgc acagatgttt gcagcaagga tacctgggtc gatcatgagc ttcaagagct
      901 ccgctgcgcc cattccacaa ggcgtcgcag caattcctca aggacgcgcc ggggcgctgg
      961 cattggccgc gctcggcgtg gttttcggtg atatcggcac aagcccactc tataccctca
     1021 aggttgtgct tgcgtccagc ggcgcacaag ctgcggatcg agcgatggcg ctcggcagcc
     1081 tctcgctcat cttttggacg ctcatcatcg taacgtcgct caaatatgtc gccgtcgcca
     1141 tgcgggtcga taatcacggc gagggcggca tcatggcgct gatggcgctg gtcggaggcc
     1201 ggaagggtct gcgcgtccgc aacaggaagc gctcgataat catggctgcc ggcttgttcg
     1261 gcgcggcgct tatttacgga gacggtgtga ttacccccgc tatctctgtt ttgtccgcgc
     1321 tggaagggtt gtcgattgta acgacgagcc tggaagcgta tgtggtgccg gcgacggtcg
     1381 gcattcttgt gctgctcttt ctcgtgcagc cgcgcggttc agcatccatc gggaagatgt
     1441 tcggacccat catggcggtc tggttcctgt gcctcggcct tcttggattg tggggcattg
     1501 ttcagaatcc atccgtgatc gaggcggtga acccggcttt gggcttgcac tatctcgtgt
     1561 caggcggcac gacgtccttc ctggtgctgg gcgccgtctt cctctgcgtc accggtgcgg
     1621 aagcgctcta cgcggacatg ggccatttcg gccgcctgcc tattcgatgg gcctggacgt
     1681 ttattgtctt tccatgcctc gtcctgaatt atgccgggca gtcggcgctc gtcttgaacg
     1741 gcgctcctct ctccgagaac atgttttacg cgctttgccc aaggccgttg cttgttcccc
     1801 tcgtcgttct ggcgaccgcc gcgacgatca tcgcgagtca ggccatcatt accggcgcgt
     1861 tctcaatgac gaggcaggca atccagcttg gctggctgcc acgtctgcgc atcacgcaga
     1921 catccgcgct gggatatggg caaatctaca tcggtgtcgt gaactggttc atgatggcca
     1981 taaccatcgc cctcacggta gcctttcgaa aatcggaaaa tctcgccgcg gcttatggta
     2041 tcgccgtgtc cgcgaccatg ctgatgacca cggtgcttct ctacatcgcc atggttgaaa
     2101 aactgaaatg gagcgcatcg gcgagcgctc cgatagccgg aagcttcgca atcgtcgaca
     2161 gcgcctttct gattgccaac tcggccaaga tcgcggaagg cggctacgtg ccgcttgcac
     2221 tcgcagttgt catcttctcc ctgatgtgga tctggcattc cggccgcgat gccatcatgc
     2281 aggtgttaag gtccaaat
//
LOCUS       DATMGX010000902         2311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_306781, whole genome shotgun sequence.
ACCESSION   DATMGX010000902 DATMGX010000000
VERSION     DATMGX010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306781"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VNR65_14770"
     CDS             complement(<1..336)
                     /locus_tag="VNR65_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19982.1"
                     /translation="MAMIANLQYGWTYFVAPMAKANSWDVAGIQVAFSIFVALETWLT
                     PLEGWIVDSLGQRGPKLMVAAGGILVAIGWLINAKAGSLEMLYLGAVVSGIGAGGIYA
                     TCVGNSVKWF"
     gene            complement(682..1968)
                     /gene="oxlT"
                     /locus_tag="VNR65_14775"
     CDS             complement(682..1968)
                     /gene="oxlT"
                     /locus_tag="VNR65_14775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HWJ19983.1"
                     /translation="MTDMVHGAAPAAARVSDTYRWTQLAIGVAAMVMIANYQYGWTFF
                     VPDIQKKFGWDRASIQLAFTLFVLFETWLVPIEGWFVDKYGPRVVVLIGGILCAIGWA
                     INAEATTLNGYYLGMIVAGIGAGGVYGTCVGNALKWFPDKRGLAAGITAAGFGAGSAL
                     TVAPIQAMIKDSGFQSTFLYFGLGQGMIIVVLAFFLFAPKAGQVPAAVKNTHVIQTRR
                     DYEPSEVIRNPIFWLMYFMFVIVGAGGLMITANLKPIAVDWKIDNIPVTLMAMTMTAV
                     TFAATIDRILNGLTRPFFGWVSDKIGRENTMFIAFGLEGLGIWALYVFGQDPIWFVVL
                     SGLVFFAWGEIYSLFPSTCTDTFGSKFATTNAGLLYTAKGTAALLVPVANYMQQSSGS
                     WDRVFLIAASANILASLLALVVLKPWRRSVVANAIA"
     gene            complement(2074..>2311)
                     /locus_tag="VNR65_14780"
     CDS             complement(2074..>2311)
                     /locus_tag="VNR65_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19984.1"
                     /translation="QRFGNGPGQRGYFIESIAPRDVSTRAINRARSLPPERFTSCCML
                     QTAAGFARFLAWRLIIRCAQASTACDKLTLPATK"
BASE COUNT          514 a          677 c          689 g          431 t
ORIGIN      
        1 aaaccatttc acggaattgc cgacgcaggt tgcatagatg cctccggccc cgatgccgga
       61 caccacggca ccgagataga gcatctccag cgacccggcc ttggcgttga taagccagcc
      121 gatcgcaacc aaaataccac cggccgcgac catcagtttt gggccgcgct gaccgagcga
      181 gtcgacgatc catccttcaa gcggggtgag ccaggtttcc agcgcgacga aaatactgaa
      241 ggcgacctgg atgccggcga cgtcccagga attcgctttc gccatcgggg cgacgaaata
      301 agtccagcca tactggaggt tggcgatcat cgccatgcag acgacgccga tgataagttg
      361 catccagcga aagctgttgg acggtgcctg agttggcgcg tttgctgcgt tagcgatcat
      421 tgtttcctcc gaaattcgcg cttttttcaa tctgacccat gagtccgccg gcgcttcgcc
      481 ctaaagtctg gtatattata ttccaatgtg caagttcgaa tttagtcgag taggcgcgta
      541 cgctgtggcg ctacgcctcg ccaaaagaag catctccagc ccgtcacggt cacaaaaaag
      601 cgcccatccc ggcgggatgg gcgcgctatt cgcagttatg ccggcgtgac ttacgccagg
      661 gccttgtaac gcaagagcct gttacgcaat agcgttggcg acgactgagc gtcgccaggg
      721 tttgagcacg acgagcgcga gcagtgacgc aaggatgttc gcgcttgcag caatcaggaa
      781 aacccggtcc cacgaaccgg aagactgctg catgtagttc gcaactggca ccagcagggc
      841 tgcggtgccc ttcgccgtat agagcaggcc ggcattggtg gtggcgaatt tcgagccgaa
      901 tgtatcggta caggtcgatg ggaatagtga gtagatctcg ccccatgcaa agaacaccag
      961 tcccgacagc accacgaacc agatcggatc ctggccgaac acgtagagcg cccagatgcc
     1021 caggccttca aggccgaagg cgatgaacat ggtgttctcg cggccgatct tgtcggacac
     1081 ccagccgaag aacggccggg tcaagccgtt caggatgcga tcgatcgtcg cggcaaaggt
     1141 gacggcggtc attgtcatcg ccatcagcgt caccggaatg ttatcgatct tccagtcgac
     1201 cgcgatcggc ttgaggttgg cggtgatcat caagccgccg gcgccgacga tcacgaacat
     1261 gaaatacatc agccagaaga tcggattgcg gatcacctcc gagggctcgt aatcgcgccg
     1321 ggtctggatc acgtgggtat tcttcaccgc ggcaggaacc tgtcctgcct tgggcgcaaa
     1381 gaggaagaat gcgaggacga caatgatcat gccctgtccg aggccgaaat agagaaacgt
     1441 gctctggaag ccggaatcct tgatcatggc ctggatcggc gcaacggtga gcgccgaacc
     1501 cgcgccgaag cccgccgccg tgatgcccgc ggcgaggccg cgcttgtcag gaaaccattt
     1561 gagcgcgttg ccgacgcagg tgccatagac gccgccggcg ccgatacccg cgacgatcat
     1621 gccgagataa tagccgttga gcgtggtcgc ctcggcgttg attgcccagc cgatggcgca
     1681 aaggatgccc ccgatcagga ccaccacgcg tgggccgtat ttatcgacga accagccttc
     1741 gatcggcacc agccaggttt cgaacaggac aaaaagggtg aaagccagct ggatcgatgc
     1801 acgatcccat ccgaattttt tctggatgtc ggggacgaaa aatgtccagc cgtattggta
     1861 attggcgatc atcaccatgg cggcgacgcc aatggccagt tgtgtccagc gataggtatc
     1921 gctgactcgg gcggcggctg gtgctgcccc gtgaaccatg tccgtcataa atctccccac
     1981 gatacgcttc tctcaatcgg gcaagcgtta tcgcggtcat tctggtcttc gaatatgccc
     2041 gagggcaaga tttcgccggc gcgttcggcg tgttcacttt gtcgcaggca acgtcaactt
     2101 gtcgcaggcc gtggaagcct gcgcacagcg aattatcagc cgccaagcga gaaaccgcgc
     2161 gaaaccggca gccgtttgca gcatgcagca agacgtgaat cgctcgggcg gcagcgacct
     2221 cgcgcgattg atagcgcgcg tcgaaacgtc gcgcggtgca atggactcaa taaaatagcc
     2281 ccgctgaccg gggccatttc caaatcgctg g
//
LOCUS       DATMGX010000903         4614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_412488, whole genome shotgun sequence.
ACCESSION   DATMGX010000903 DATMGX010000000
VERSION     DATMGX010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412488"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /gene="dnaN"
                     /locus_tag="VNR65_14785"
     CDS             <1..1061
                     /gene="dnaN"
                     /locus_tag="VNR65_14785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWJ19985.1"
                     /translation="ERRNTIPILSNILIGADGGGSLTLKATDLDMEAVESVPAKVETA
                     GAVTVPAHTLHDIVRKLPEGAEIKIERKGDEGRLKVAAGRASFELAILPADDFPDMGQ
                     AELPSRFTVGSGELKRLFEKARFAISTEETRYYLNGIYLHSIESDGSKKLRAVATDGH
                     RLAQVQTDCPSGADGMPGVIVPRKAVGEVIKLLESDHAPVEIALSQAKIKFTVGELVL
                     TSKLIDGSFPDYARVIPTTNKKHLLLENADLVKAVDRVSTLSSEKGRAVKLNASEGRL
                     VVSASNPESGSATEEISADYRNEPIEIGFNARYLLDITAQIDGSQTLFKLADGSSPTL
                     ISDESDASALYVLMPMRV"
     gene            1108..2298
                     /gene="recF"
                     /locus_tag="VNR65_14790"
     CDS             1108..2298
                     /gene="recF"
                     /locus_tag="VNR65_14790"
                     /inference="COORDINATES: protein motif:HMM:NF000820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HWJ19986.1"
                     /translation="MEPSSRLWIGKLTLTDFRNYRASTMTVRPGPVVLVGPNGAGKTN
                     CLEAVSLLTAGRGLRSLPFSDLARGGGPGGWAIAARLSVAGEEVEIGTGVQPSADGIL
                     SARAARIVKIDHAIAKGSGALAAVRMLWLTPAMDGLFTGPASERRRFLDRLVLSLEPS
                     YASAAAIFDRAMRQRNKALETLSPPQLLNAIEAQMADAAVSIAAARTRAIEDLASEIL
                     EERDRDPDSVFPWAGLTLQGHLEGQVGTMDKGAMSDAYRLLLASARDRDRAAGRTLAG
                     PHRSDLEVAHGPKGTPAKMCSTGEQKALLIGLVLAHARLIKKTCGGVSPVILLDEIAA
                     HLDLGRRLALFSSIASLNAQVWMTGTDADIFAPLQNVTETQFFHVLSGAIVPANDAES
                     ASEH"
     gene            2348..>4614
                     /locus_tag="VNR65_14795"
     CDS             2348..>4614
                     /locus_tag="VNR65_14795"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HWJ19987.1"
                     /translation="MEAARRMDAAAEAYGADSIKVLKGLDAVRKRPGMYIGDTDDGSG
                     LHHMVYEVVDNAIDEALAGYADLVTVALNADGSVTVEDNGRGIPTEIHTEEGVSAAEV
                     IMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSTRLELTVWRGGHEHFMAFADGVPE
                     EPLKIVAKTPGKSGTRVTFTPSPATFTMVEFDYATLEHRIRELAFLNSGVNINLLDLR
                     GVEPVKSEFKYDGGLREFVQYLDRARTPLLAEPIAIKAERLLDGSPITKDTDPGEPRI
                     TIEAALWWNDGYHEGVFCFTNNIPQRDGGTHLAGFRAALTRTVNTFATSSGIAKKEKV
                     QLTGDDAREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPAVEGIMTEALGGWFEEHPV
                     ECKLIILKVAEAAAAREAARKARELTRRKGVLDVANLPGKLADYQERDPRKSELFLVE
                     GDSAGGSAKQGRSRSNQAVLPLRGKILNVERARFDKMLSSQEIGTLITALGTGIGRDD
                     FDIAKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPQLLENGHLYIAQPPLYKVKRG
                     SSENYLKDERAFEDFLIDGGLDGAILKAEGGEHRSGRDLRSNVERARSFAHVLDGLHM
                     RYSRTVVEQAAIAGLLNPKLVNDEGEAERLAGMVVERLKALSEETEQGWSWLRGSDGG
                     FVFSREVRGVRESHNIEWALIQSADARKLHTYHAEFSELFSGKLVLERKSAQTPVFGP
                     RSLLAAVFAAGRAGLT"
BASE COUNT          929 a         1377 c         1423 g          885 t
ORIGIN      
        1 tcgaacggcg taataccatc cctatcctct cgaacatcct gattggcgcc gatggcgggg
       61 gcagcctcac gctgaaagcg acagacctcg acatggaggc cgtagaaagc gtccctgcga
      121 aggtcgagac ggccggcgct gtgacggtcc cggcgcatac gctccatgac atcgtccgaa
      181 agctccctga aggagcggag atcaagatcg aacggaaagg cgacgaaggc cgtctgaaag
      241 tcgccgccgg acgggcaagt ttcgagcttg cgattctgcc cgccgatgac tttccagaca
      301 tgggccaagc cgagttgccc agccgtttca cagtcggaag cggtgagctg aagcgccttt
      361 ttgagaaggc gcgctttgcc atttcgaccg aagaaacgcg gtattacctg aacggcattt
      421 atcttcattc catcgagagc gatggcagca agaagcttcg ggctgtggcg accgatggtc
      481 acaggctggc gcaagtgcag accgattgtc cctccggtgc ggacgggatg cccggcgtga
      541 tcgtgcctcg aaaggcagtt ggagaagtca tcaagcttct cgaaagcgat catgcccccg
      601 tcgagatcgc gctctctcaa gcgaaaataa agttcaccgt tggcgagttg gtcctcacct
      661 caaagctgat cgacggttcc ttcccggact acgcgcgggt gataccgaca acgaacaaga
      721 agcatctgct tcttgaaaat gcggatctgg ttaaggcggt ggaccgtgtt tcgacgctct
      781 cctcggaaaa ggggcgcgcc gtcaaactca atgccagcga gggccgtctc gtcgtgtcgg
      841 cgagcaatcc cgaatcgggt tccgccaccg aggaaatctc cgcagactat cgcaacgagc
      901 ccatcgaaat tggcttcaac gcgcgctatc tgctcgatat cacagcgcag atcgatggct
      961 cgcagacgct gttcaagctg gccgatggtt cttccccgac ccttatttca gatgagagcg
     1021 atgcgtccgc attgtatgtg ctgatgccga tgcgcgttta gccctgactt acgcttcgat
     1081 aagacattgc attctgcgcc gcaggtattg gaaccatcat cgcgactctg gatcggcaag
     1141 ctgacgctca ccgattttag aaactatcgc gcctcaacga tgacggtgcg acccggcccg
     1201 gtcgttctcg tcggccccaa tggcgcgggg aaaacgaact gcctcgaggc tgtttcgctt
     1261 ctgacggctg ggcgtggctt gcggtcgctg ccattctctg acctcgctcg aggcggtggc
     1321 ccgggcggtt gggcgattgc cgcccgcctc tcggtcgccg gcgaggaggt cgagattgga
     1381 accggcgttc agccttctgc agatggaatc ttgagcgccc gcgccgcgcg catcgtgaaa
     1441 atcgatcatg ccatcgcgaa aggatccgga gcgcttgctg ccgttcgcat gctatggctc
     1501 acgcccgcca tggatggcct gtttaccgga cccgccagcg agcgccgccg cttcctggac
     1561 cggctggtcc tcagtctgga gccctcctac gccagcgccg ccgccatctt cgaccgcgcc
     1621 atgcggcagc gaaacaaggc gctggaaacc ttgagtccgc cgcagctttt gaatgcgatc
     1681 gaggcgcaaa tggcggacgc tgccgtgagc atagccgcag cccgtacgcg ggccatcgaa
     1741 gaccttgcct ccgaaatcct ggaggagcgc gaccgcgatc ccgactcggt gttcccttgg
     1801 gccgggctga cgctgcaggg tcatctggaa gggcaggtcg gcacgatgga caaaggcgcg
     1861 atgagcgatg cgtatcgcct gcttcttgcc agcgcgcgcg accgcgaccg cgccgccgga
     1921 cgcacgctgg caggtccgca ccggagcgac ctcgaggtcg ctcatggacc caagggcaca
     1981 ccggcaaaga tgtgctcgac gggcgagcag aaggctctcc tcatcggtct tgttctcgcc
     2041 catgcccgcc tcatcaagaa aacctgcggt ggcgtttccc ctgtgatttt acttgatgaa
     2101 atcgccgcgc atctcgatct tggccgccgc ctcgctctct tctcgtccat cgccagcttg
     2161 aacgctcaag tttggatgac ggggaccgat gcggacatat ttgcgcccct tcagaatgtc
     2221 acagaaaccc agtttttcca cgttttaagc ggcgctatcg tgcccgcaaa cgatgcagaa
     2281 tccgcttcgg aacactaaat aatagaaaag caagtccgcg agatccgcgg ctgatcaggt
     2341 gcattttatg gaagcagcaa gacgcatgga cgcggcggcg gaagcctatg gcgccgacag
     2401 tatcaaggtc ctgaaaggcc tcgacgcggt ccgtaagcgt cccggcatgt atatcggcga
     2461 cacggacgac ggctcgggcc ttcatcacat ggtctatgaa gtggtcgaca acgccatcga
     2521 cgaggcgctc gcgggctacg ccgatctcgt aaccgtggcg ttgaacgcgg acggttccgt
     2581 gaccgtggaa gataacgggc gcggcatccc aacggaaatc cataccgagg aaggtgtctc
     2641 cgccgccgaa gtcatcatga cccagcttca tgcgggcggc aaattcgacc aaaattcata
     2701 caaggtttcc ggcggcctgc acggagtcgg cgtgtcggtt gtaaatgcgc tttcgacgcg
     2761 gcttgagctc acggtctggc gcggcggcca cgagcatttc atggcgtttg cggacggcgt
     2821 ccccgaagaa ccgctgaaaa tcgtggccaa aacgccaggc aagtccggca cgcgcgtcac
     2881 cttcacgccg agccctgcaa ctttcacgat ggtggagttc gattacgcga cgctggagca
     2941 tcgcattcgc gagttggcgt tcctgaactc cggggtcaac atcaaccttt tggatctgcg
     3001 cggcgtcgag ccggtgaaga gcgagttcaa atatgacggc ggcctcaggg agttcgtcca
     3061 atatctcgac cgggcgcgca cacctttgct cgccgagcct atcgccatca aggccgagcg
     3121 gttgctggac ggttccccga taaccaagga caccgatccg ggcgaaccac ggattacaat
     3181 cgaggcggcg ctgtggtgga acgatggcta ccacgaaggc gtattctgct ttacgaacaa
     3241 catcccgcag cgggacggcg gaacgcatct tgccggtttt cgcgcggccc ttacacgcac
     3301 ggtcaacacc ttcgccacgt cttctggcat cgccaagaag gagaaggtgc aactcaccgg
     3361 cgacgacgcg cgcgaggggc taacctgtgt cctatcggtt aaagtgcccg acccgaaatt
     3421 ctcaagccag accaaggaca agctggtgtc ttcggaagtg cggcctgcgg tcgaaggcat
     3481 catgacggag gcgcttggcg gatggttcga ggagcacccc gtcgaatgca agcttattat
     3541 cctgaaagtg gccgaggcgg cggcggctcg cgaggcggcc cgcaaggcgc gcgagttgac
     3601 gcggcggaag ggcgtgctgg acgtggccaa ccttcccggc aagctcgcgg attatcagga
     3661 gcgcgatcca aggaagtcgg agctattcct cgtcgagggc gatagcgcgg gcggttcggc
     3721 caagcaaggc cgttcccgct ccaatcaggc tgttcttcca ctgcgcggca aaatcctgaa
     3781 cgtcgaacgg gcgcgcttcg ataagatgct ctcaagccag gagatcggga cgctgatcac
     3841 ggcgctcggc accggcattg gccgcgacga cttcgatatc gcgaagctgc gctaccatcg
     3901 catcatcatc atgacggacg ccgatgtgga cggcgctcac atccgcacgc tgctgctgac
     3961 ctttttctat cgccagatgc cgcagctatt ggaaaacggc catctctata tcgcacagcc
     4021 gccgctctac aaggtcaagc gcggcagctc agaaaattat ctgaaggatg agcgcgcttt
     4081 cgaagacttc ctgatcgatg gcggcctcga cggagccatt cttaaagcgg aggggggcga
     4141 gcatcgatcg gggcgggatc ttcgctcaaa tgtcgagcgc gcgcgatcct ttgcacatgt
     4201 gctggatggg ctgcacatgc gctattcccg cactgtggtg gaacaggctg ccattgctgg
     4261 tcttctcaat ccgaagctcg tgaatgatga aggagaagcg gaacgcttgg ccggaatggt
     4321 ggtggagcgt ttgaaggccc tctcggagga gacggaacag ggctggagct ggctccgcgg
     4381 ctccgatggt ggcttcgtct tttcgcggga agtccggggc gttagggagt cgcacaacat
     4441 cgagtgggcg cttatccaat ccgcggatgc tcgaaagctg catacgtacc atgccgagtt
     4501 ctctgaattg ttctcgggca agctcgtgct ggagcggaag agcgctcaaa cacctgtgtt
     4561 tgggccgcgc agccttttgg ctgcggtatt cgcggcaggc cgggcagggc ttac
//
LOCUS       DATMGX010000904         2073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_179216, whole genome shotgun sequence.
ACCESSION   DATMGX010000904 DATMGX010000000
VERSION     DATMGX010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179216"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..636)
                     /locus_tag="VNR65_14800"
     CDS             complement(7..636)
                     /locus_tag="VNR65_14800"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HWJ19988.1"
                     /translation="MTLFERWLTLWVALCIVVGIVLGHLFPGVFHTIDSAEIAKVNLP
                     VAALIWLMIVPMLIKIDFAALSQVKEHWRGIGVTLFINWAVKPFSMAALGWFFIGYVF
                     RPYLPAGQIDSYIAGLIILAAAPCTAMVFVWSNLTKGEPHFTLSQVALNDTIMVFAFA
                     PIVGLLLGLSSITVPWNTLILSVVLYIVVPVILAQMLRRRLLAAGARKL"
     gene            complement(683..1054)
                     /locus_tag="VNR65_14805"
     CDS             complement(683..1054)
                     /locus_tag="VNR65_14805"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWJ19989.1"
                     /translation="METEHAILALAALAQSTRLEVFRLLARHEPDGLAAGDIARALAV
                     PQNTMSSHLSILTRAGLVSSERHSRSIIYRANLDGFQSVALFLLKDCCGGRSEVCAPL
                     IDSLSPSCPPKKGKANVRARA"
     gene            1435..>2073
                     /locus_tag="VNR65_14810"
     CDS             1435..>2073
                     /locus_tag="VNR65_14810"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HWJ19990.1"
                     /translation="MGFGLDRRLDKRELLGATVSLLLASTVSSAAETPANGPIRFGLT
                     PVFLTNDLELLGRLQSYLQRATGHPVELITRRTYQEITTLLTTGQLDAAWICGFPYVA
                     HRTELQLVAVPLWHHKPLYQSYLIVDRDRKGSGILDLRGDIHAFSDPDSNSGFLVTRA
                     ALAERGLKPETFFTKTFFTYAHRDVIRAVASGLAGSGSVDGYIYDVVAEIDPD"
BASE COUNT          472 a          595 c          580 g          426 t
ORIGIN      
        1 gcctactcag agcttcctgg cccccgccgc aagcagccgg cggcgcagca tttgcgcaag
       61 gatcacgggc accacaatgt acagcacgac cgacaaaatc agagtgttcc aaggcacggt
      121 aatcgacgac agccccagca gcaggcctac aatcggcgcg aatgcgaaaa ccatgatggt
      181 gtcgttgagc gctacctggc ttagtgtgaa atgcggctct cccttggtga ggttggacca
      241 gacaaagacc atcgcggtac aaggcgctgc ggcgaggatg atcaatccgg cgatgtagct
      301 gtcaatctga ccggccggca gatatggccg gaacacgtag ccgatgaaga accagcctag
      361 cgctgccatc gagaacggct ttaccgccca gttgatgaaa agcgtcacgc cgatcccgcg
      421 ccagtgctcc ttgacctggc tcaaggcggc gaaatcgatc ttgatcagca tcggtacgat
      481 catcagccag atcaacgctg caaccggaag gttgaccttg gcgatttcgg ctgaatcgat
      541 ggtgtggaag acaccgggaa acaggtggcc aagcacgatg ccaaccacga tgcagagcgc
      601 cacccacagg gtcagccaac gttcgaaaag tgtcatgtcg gttccttctc aggcggagcg
      661 ggcagaattt ccgaaacgaa cgttatgcac gcgctcggac attcgctttt cccttcttcg
      721 gcgggcaact aggtgaaagg ctatcgatca gcggcgcaca gacttcagac cgaccgccgc
      781 aacagtcctt gagcaggaaa agagcgacac tctgaaagcc gtccagattg gcgcgataga
      841 tgattgagcg gctgtggcgc tccgaggaca ctaagcccgc ccgcgtcaga atcgacaggt
      901 gagaagacat cgtgttttga gggaccgcca gagcgcgcgc gatatcgccg gccgccaaac
      961 cgtcgggttc gtgcctagcc aaaagccgaa aaacctcaag ccgcgtcgac tgggcgagcg
     1021 cagccaacgc gagaatggca tgttctgttt ccatccatcg ataattctcg atatatggga
     1081 aagagccaag ccggcggcgg ccctgatctc atcaattcgg tcgtagaata ccgcgtacca
     1141 tctccgtatt cataagagtc tctcttggcg aagactgatg atgtcgccaa acctcaagct
     1201 tcgggaatcc gacgacactc cagttctatc gggcgctggt gcgccgttga ggccagtttg
     1261 tcgttcagga gccagcaagt ttagccgttt tggggagttc cgctgcaggg caaaggcgag
     1321 tttgggtgcg cgctagtaac ctccgcgcaa tcacgtcacc gcgttggcag cctgtttcaa
     1381 cgggtctcga tccgcgctaa cataagaaaa aacgttagaa atctgggagg ggtcatgggc
     1441 ttcggtttgg accgccgatt agacaaacgc gagcttttgg gcgcgactgt atcactgctg
     1501 ctggcgtcaa cggttagcag cgcagcagaa actccggcta acggacctat tcgatttggc
     1561 ctcacgcctg tttttctcac caacgatctt gagcttctcg ggcgcttgca atcgtacctc
     1621 cagcgcgcga ccgggcatcc ggtagagcta attactcggc gcacctatca agaaatcact
     1681 actctgttga ccacgggcca actcgatgcg gcgtggattt gcggctttcc ctatgttgcg
     1741 catcgtaccg aacttcagct tgtcgcggtg ccgctgtggc atcacaagcc actttatcag
     1801 tcttacctga tcgtcgaccg tgaccgcaaa ggcagcggca tcctcgatct gcgtggtgat
     1861 attcatgcgt tttccgatcc tgattcaaat tcaggatttc ttgtcacgcg agcagccctc
     1921 gccgaacgag gtctcaagcc agaaactttc ttcacgaaaa cgttcttcac ctacgcccat
     1981 cgcgatgtga tccgagcagt cgcgtctggg cttgctggat caggatcagt ggacggctat
     2041 atttatgacg tcgttgccga gatcgacccc gat
//
LOCUS       DATMGX010000905         2651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_359857, whole genome shotgun sequence.
ACCESSION   DATMGX010000905 DATMGX010000000
VERSION     DATMGX010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359857"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VNR65_14815"
     CDS             complement(<1..618)
                     /locus_tag="VNR65_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ19991.1"
                     /translation="MLQDRHGVIDYSEKGAGPTIVFVPGSWATMSAWRGVIAALDGRF
                     RTVTTSLRGYGGTRECRTRTDTSLDRQAEIVEAVIRRVGAPVHLVGHSYGGLVCLDVA
                     LCGLVPLMSLTLIEPVAFGLLRQEGELALHEQFIAMREDYVRSCENGDKEAARRVVDY
                     LDGRGSFDTLPYWMREYIVETTPTHVLDIRSSFDSPLSALANILLP"
     gene            1030..2595
                     /locus_tag="VNR65_14820"
     CDS             1030..2595
                     /locus_tag="VNR65_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ19992.1"
                     /translation="MRYLFEDFALDVDKRELFRGADAVSISPQALDLLVCLIRNRERV
                     ISKDDLISAIWGGRIITDAALATRLNSARVAIGDTGEKQRLIKTLQRKGFRFVGTVLE
                     VRKPDDATAASGAAQRPEIPDKPSIAVLPFENMSGDPEQEYFGDGLVDDIITALSRFR
                     ALFVIARTSSFTYKGKIVDIKQIGDELGVRYVLEGSVRKAGTRLRINGQLIDVTTGAH
                     LWADRFDGTLDDIFDLQDKVTQQVVGAIAPEVDRAEIERASRKSVGNIDAVTAYYRGL
                     PHIQFPTSPANNDAALRDFHDAMALDPGFMPAYGGAAGCLGWRRANNWPGSVEKDNAE
                     LLRLAERVKELGTDDALALGVLGFNLFWMLLDFDRGLEMVEQAIQSNPNYARAYNYRG
                     LLRAWHGASEAAVADLEQAMRLSPRDPVNYNAMMGLAVAHHNAGRHHEAAEWADKSLR
                     AFPPAFFVGMTQGILCYVAAGRMEDARKLMAECLRLVPKWRRSTFVAPQWFRSAKLRA
                     ELVEAFIAAGLPE"
BASE COUNT          560 a          779 c          766 g          546 t
ORIGIN      
        1 cggaagcaat atattggcca aagccgagag ggggctatcg aagctcgagc gaatgtccag
       61 aacatgggtc ggcgtggttt caacaatata ctctcgcatc caatacggga gagtatcaaa
      121 gcttccccgg ccatcgagat aatcgaccac ccggcgcgcc gcttctttgt caccattttc
      181 gcacgaccgt acatagtcct cacgcatcgc gataaactgc tcatggagcg cgagttcacc
      241 ctcttggcgc aaaaggccga acgccaccgg ctcgatcaag gtcagactca tgagggggac
      301 aagtccgcac agcgcgacat ccaggcagac tagtcctcca taggaatgtc cgacgaggtg
      361 cacaggagca cctacgcgac gaatcacagc ttcgacaatc tctgcctgtc ggtccaatga
      421 cgtgtcggtt cgcgtccggc attcccgcgt gccgccatac ccacggaggc tcgtcgtgac
      481 ggtccggaag cgaccgtcca gtgccgcgat cacaccgcgc caggccgaca tggtggccca
      541 tgaaccgggc acgaacacga ttgtcggccc tgcccccttt tcgctgtagt cgataactcc
      601 gtgcctatct tgcagcatct gcgtctcccg ggctgcgcat aatcgcgaac gggcctgccc
      661 agacgtccgg gcggtgcagg ccaaggtcgt caagctcgaa gtcgctgagc gcggaggtct
      721 ctgctcttcg cctacggcgc cggcgtaagg ctgagtcggc ccttgacggg accttgcgca
      781 atagctgaag cgccccgcat cgtgtccgaa aaagctcagg ggctccgatt ggccgggtag
      841 cctcgcttgc ccaaaaggtc gccttcattg ttacgatccc cggtcggata acttccgtct
      901 gaggagacga tgcacgaggc gcaggtccga cactttacta gccgcttaca tttgctttac
      961 tgccgactga tttcaggcct acgaggcgag cccgatctca ctcttatcca gtaggaagga
     1021 gttcctagat tgcgctattt gtttgaagac ttcgcgcttg atgtcgacaa acgggagcta
     1081 tttcggggag ccgatgctgt ttcgatttcg ccccaggcac tcgatcttct tgtttgcctg
     1141 atccgcaacc gcgagcgcgt gatcagcaag gacgacttga tctccgcgat ctggggaggc
     1201 cgcatcatca ccgacgcagc gcttgctacg cgccttaact cagcccgtgt cgccatcggg
     1261 gacaccggcg agaaacagcg tcttatcaaa acgctgcaac gcaagggctt tcgtttcgtt
     1321 gggacagtgc tggaggtccg gaaacccgat gatgccacag ccgccagcgg cgcagctcaa
     1381 cggccggaaa ttcccgacaa gccatcgatc gcagttcttc cattcgaaaa catgtcaggc
     1441 gatccggagc aggaatactt cggcgacggc ctggttgacg atatcatcac tgcgctctca
     1501 cgatttagag cactgttcgt aatcgcccgc acttcgtcgt tcacctacaa gggaaagatc
     1561 gtcgatataa agcagattgg agacgaactc ggtgtccgct acgtgctgga aggaagtgtg
     1621 cgcaaggccg gtacacgcct aaggatcaac gggcagctca tcgatgtcac gacaggcgcc
     1681 cacctctggg ccgatcgatt tgacggcacc ctggacgata tttttgatct tcaggacaaa
     1741 gtcactcagc aggtcgtcgg cgcgattgcg ccggaagtcg accgcgccga gatcgagcga
     1801 gcaagccgaa aatcggtcgg caatatcgac gcggtgactg catattaccg tggtttgccc
     1861 catatccaat ttccaaccag cccagcgaat aacgacgccg ctttgaggga tttccatgac
     1921 gcaatggctc tcgatccggg ttttatgcca gcctacggcg gcgctgcggg ttgtcttgga
     1981 tggcgtcggg ctaacaactg gcccgggagc gtcgagaaag acaatgctga actcttgcgc
     2041 ctggcggagc gcgtcaagga attgggcaca gacgatgccc tcgctctagg tgtcctgggc
     2101 ttcaatcttt tctggatgct gctggacttc gatcgcgggc ttgaaatggt cgagcaggcg
     2161 atccaatcga acccaaacta tgcgcgcgca tacaactaca ggggactgtt gcgcgcctgg
     2221 catggcgcgt ccgaggcggc ggttgccgac cttgagcaag cgatgcgtct cagtcctcgc
     2281 gatccggtca actacaatgc gatgatgggg cttgccgttg cgcatcataa cgccggcagg
     2341 caccacgaag cagctgagtg ggccgacaaa tcccttcgag cgtttccccc cgcctttttt
     2401 gtaggaatga cacaaggtat cctgtgctac gtggcggcag gaagaatgga agacgctcga
     2461 aaattgatgg cggaatgcct tcgtctggtt ccgaaatggc gtcgttcgac attcgttgct
     2521 ccacaatggt ttagatcggc caaacttaga gcggagttag tggaagcgtt cattgctgcg
     2581 ggactgcccg agtaggcctg ccttttgccc taccgaagga gatgcgggcg gatcggccat
     2641 tccctggcat g
//
LOCUS       DATMGX010000906         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_338991, whole genome shotgun sequence.
ACCESSION   DATMGX010000906 DATMGX010000000
VERSION     DATMGX010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338991"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VNR65_14825"
     CDS             <1..1379
                     /locus_tag="VNR65_14825"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWJ19993.1"
                     /translation="AGKKIAVKKYVVRLSAEEREQLASLIRSGNRAAQLLTKARIPLK
                     ADISEAGEGWSDDKIAEALDTSVANVERTRRQLVEEGFEAVPTRKYNPNSAPPRIFDG
                     VAEAKLIALLVFYEILLPFAAGFVLAYLFQPFVERMNGWGLDRGVAAFIIIAAVALIF
                     AIIAALIVPPLVDQLVQLIQEVPRYYQEARAYALQHYGNYLAPFQKQPGGPRASGPQM
                     PGGQFTQDLAPWLVNHLQTLLQGSLALFNSLALMFLTPVVTFFLLRDWDKMIAGIEKL
                     LPRQDAPAVRKIAQQIDFTISGYFRGMLIVLLIVSAFYMVALGAIGLNYGLVIGLGAG
                     LISFVPYVGSTSGLLVSGGVALSQFWPDYTRIALVCGVFVLGQIIEGNVLTPKIVGDK
                     VRLHPVWMLFALIASGYLFGFTGLLISVPMAAAIGVLVRFAVRKYYGSPIYEGEEKNE
                     RKAETIRG"
     gene            1389..2096
                     /locus_tag="VNR65_14830"
     CDS             1389..2096
                     /locus_tag="VNR65_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HWJ19994.1"
                     /translation="MSSQLALDLPHRQSWDEADFFITSRNEHAMGLVTAWPDWHAPAA
                     VIFGPPQSGKSYLANIWRNRSEGMFIETSHLAEHLWAAPYTPLIVEDVDTSAFCETAL
                     FHHLNLAREHSSFILLTAQTPPGQWEISLPDLRSRIRSYPAAEIQPPDQEHLAGLLLK
                     HFSDRQIEVAPDVISYLVARIERSMAAAQTVAERLDKLALAEQKKVTRAFAAKVLKDL
                     NAAVADGESGDQAEGDS"
     gene            2243..>2403
                     /locus_tag="VNR65_14835"
     CDS             2243..>2403
                     /locus_tag="VNR65_14835"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HWJ19995.1"
                     /translation="MREAQETSRPSPEALLELARSQEAGRGRLKVFLGAAPGVGKTYE
                     MLLSARSRRA"
BASE COUNT          431 a          751 c          734 g          487 t
ORIGIN      
        1 tggccgggaa aaagattgcc gttaagaaat acgttgtgcg gctgagcgca gaggagcgcg
       61 agcagcttgc gtccttgatc cgctcgggaa atcgggcagc gcaattgctg acgaaggcgc
      121 gcataccgtt gaaagcggat atctcggaag ctggggaagg ctggagcgac gacaagatcg
      181 ccgaggctct ggacaccagc gtcgccaatg tcgagcgcac gcgacggcaa ttggtggagg
      241 aagggttcga ggcggtgccg acccgcaaat acaatccgaa ctccgcgccg ccccggattt
      301 tcgacggcgt ggcggaagca aagctgatcg cgctgctcgt cttttatgag atccttctgc
      361 cgtttgccgc cggcttcgtg cttgcctatc tgtttcagcc cttcgtcgag cgcatgaacg
      421 gctggggcct cgatcgtggt gttgctgcgt tcatcatcat tgcggccgtg gcgctcatct
      481 tcgcgattat cgccgcgctg atcgtgccgc ccctcgtcga tcagcttgtt caactcatcc
      541 aggaggtgcc gcgctattac caggaggctc gggcctacgc cctccagcac tacggcaatt
      601 atctagcgcc gttccagaag caacccggcg gcccgcgagc gtccggcccc caaatgccgg
      661 gaggccaatt cacgcaagac ctggcgccat ggcttgtcaa ccatcttcag accctgcttc
      721 aaggcagcct cgccctcttc aactcgctgg cgctgatgtt cctgacgccc gtcgtcacgt
      781 tcttcctcct gcgcgattgg gacaagatga tcgccgggat cgagaagctg cttccgaggc
      841 aggatgcgcc ggccgtcagg aaaatcgccc agcagatcga cttcaccatc tccggctatt
      901 tcaggggcat gctgatcgtg ctcctgatcg tatccgcctt ctacatggtg gcgcttggcg
      961 cgatcggcct caactacggc ctcgtcatcg ggctgggcgc ggggctcatc agctttgtgc
     1021 cctatgtcgg ctccacgagc ggtctcctcg tttcaggcgg cgttgcgctt tcgcaattct
     1081 ggccggacta cacgcggatt gcgcttgttt gcggcgtctt cgtgctcggc cagatcatcg
     1141 aaggcaacgt gctgacgccg aaaatcgtcg gcgacaaggt gcggctgcat ccggtctgga
     1201 tgctgttcgc cttgatcgct tccgggtatc tcttcggctt caccggcctt ctgatttcgg
     1261 tgccgatggc cgcggccatc ggggttctgg tgcgtttcgc cgtccggaag tattatggca
     1321 gccccatata cgaaggcgag gagaagaatg agcgcaaggc ggaaaccatt cgtgggtagc
     1381 gctgcgtgat gtcttcacaa ctggcgctcg accttccgca ccggcagagc tgggatgaag
     1441 ccgacttctt cataacatcg cgcaatgagc acgcgatggg gctcgtcacg gcctggcccg
     1501 actggcacgc accggctgcg gtgattttcg ggccgcctca gtcgggaaaa agctacctcg
     1561 cgaatatctg gcgaaaccgg tcggagggga tgttcatcga gacgtcgcac ctcgcggagc
     1621 atctctgggc tgcgccctac acgccgctga ttgtggagga tgtggataca tccgccttct
     1681 gcgagacggc gcttttccat cacctcaacc ttgcgcgtga gcatagctcg ttcatcctgc
     1741 tgacagcgca gacgccgccg gggcaatggg agatttccct ccccgatctg cgctcgcgca
     1801 tccggtccta tcccgcagcg gaaatccagc cgccggacca agagcatctg gcgggcctgc
     1861 ttctcaagca tttcagcgac cgccagatcg aagtcgcccc cgacgtcatt tcctatctcg
     1921 tggcacgcat cgagcggtcc atggctgccg cgcaaaccgt ggcagagcgt ctggacaagc
     1981 tcgcccttgc cgagcaaaaa aaggttacgc gcgcttttgc ggcgaaggtc ctgaaagacc
     2041 tgaacgctgc cgttgcagac ggagaaagcg gtgatcaggc cgagggtgac tcgtagggca
     2101 gggcgcacgg acggcctgcg acaaaccggc cgccatgggc gtcgctcgcg cgctgtattc
     2161 ctaaatcata atccagcgcg ctgttgctgc gctcaggatg tcgaaaccct tccgtctgag
     2221 tgacggcccc caaggtgcaa ccatgcgcga ggcccaagag accagccgac catcgcccga
     2281 agccttgctc gaattggcgc gcagccaaga ggccgggcgc ggccgcttga aggttttcct
     2341 gggcgctgct cctggcgttg gcaaaaccta tgaaatgctg ctttccgcgc gctcgcgtcg
     2401 tgc
//
LOCUS       DATMGX010000907         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_347048, whole genome shotgun sequence.
ACCESSION   DATMGX010000907 DATMGX010000000
VERSION     DATMGX010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347048"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /gene="pnp"
                     /locus_tag="VNR65_14840"
     CDS             complement(<1..1861)
                     /gene="pnp"
                     /locus_tag="VNR65_14840"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWJ19996.1"
                     /translation="MKPEATSVTVHLSGGKEISFETGKLAKQAGGAAVVRLGENVVLA
                     CATANADPREGIDFFPLTVDYREYTYAGGRFPGGFIKREGRMSEREVLTSRQIDRPVR
                     PMFAEGFKCETQIIAFVLSADSNNDPDVLGINGASCALTLSDIPFLGPIGAVRVGLVD
                     GQFIVNPTYDEMRGGLVNIMVVGTADGIVMIESGAKEVKEETVVDAIEFGHTEIKKIC
                     KAISELRDKVGKPKRKVEPPVFDDAYFNALQKKVGAEVADALDTKKHPKAESYTLVKE
                     IKKRLAAELPTTGSEKEDEVAKKKLSHYYETLRERIFREQVIKQKRRPDARAFDEVRE
                     IWIEAGVLPRTHGSSIFTRGETQALVTATLGTSDDMQRLEGFEGESKKRFMLHYNFPP
                     FSVGEVGFMRGAGRREIGHGALAERAVSAVLPTEEKWPYAMRVVSDILESNGSSSMAT
                     ICGASIALMDAGVPLKAPVAGVAMGLVKEGDQYAILTDIAGAEDHYGDMDFKVAGTKE
                     GITALQMDIKVAGITAQIMREALAQAQRGRMHILGKMEEVITTGREKISDYAPRFYTV
                     QIPVDKIRDLIGPGGKMIRSIVEQTGCKIDVEDDGSVKIYSSDGVAADRCIQMI"
BASE COUNT          377 a          643 c          599 g          468 t
ORIGIN      
        1 tgatcatctg gatgcagcgg tcagccgcca cgccgtccga ggagtagatt ttcacgctgc
       61 catcgtcttc gacgtcgatt ttgcaaccgg tctgctcgac gatgctgcga atcatcttgc
      121 cgcccggtcc aatcagatcg cgaatcttgt cgaccgggat ctgcaccgtg tagaagcgcg
      181 gcgcgtaatc ggaaatcttc tcgcgccccg tggtgatgac ttcttccatc ttgcccagga
      241 tgtgcatccg tccacgctgt gcctgcgcca gcgcctcgcg catgatctgc gccgtgatgc
      301 cggcaacctt aatatccatc tgcagcgccg tgatgccttc cttcgtgccg gcaactttga
      361 agtccatgtc gccgtagtga tcttccgcgc ccgcaatgtc ggtcaggatg gcgtactgat
      421 cgccctcctt caccaacccc atcgccactc ccgccaccgg agccttcaac ggaacgcccg
      481 catccatcag cgcaatcgaa gccccgcaga tagtcgccat cgacgacgac ccgttcgatt
      541 ccagaatgtc agacacgacg cgcattgcat aaggccactt ctcttccgtc ggaagcacgg
      601 ctgaaaccgc ccgctccgcc agcgccccgt gcccaatctc ccgcctaccc gccccgcgca
      661 taaacccaac ttctccaact gaaaacggcg gaaaattata gtggagcata aagcgtttct
      721 tggattcgcc ttcgaagcct tcgaggcgct gcatgtcgtc gctggtcccg agtgttgcag
      781 tgaccagtgc ttgggtttcg ccgcgggtga agatcgagct gccgtgggtt cttggcagga
      841 ctcccgcttc gatccagatt tcgcggactt cgtcgaaggc gcgggcgtct ggacggcgct
      901 tctgcttgat cacctgctca cggaagatgc gttcgcgcag cgtctcgtag taatgcgaga
      961 gtttcttctt ggcgacctcg tcttcctttt cgctgcccgt cgtgggcagt tcggcggcca
     1021 ggcgcttctt gatctccttg accaaggtgt agctttccgc cttgggatgt ttcttggtgt
     1081 cgagggcgtc cgccacttcg gcgccgacct tcttctgcag agcgttgaag tacgcgtcat
     1141 cgaacactgg cggctcgacc ttgcgcttcg gcttgcccac tttgtcgcgc agctcgctga
     1201 tggccttgca gattttcttg atctcggtgt ggccgaactc gatcgcgtcg acgaccgttt
     1261 cttctttcac ttccttcgcg ccggactcga tcatcacgat gccgtcggcc gttccgacga
     1321 ccatgatgtt gaccaggcct ccacgcatct cgtcatacgt gggattcaca atgaactgtc
     1381 cgtcgaccag gcccacgcgc acggcgccga tgggaccgag gaacggaatg tcagaaagcg
     1441 tcagcgcgca cgaggcgccg ttgatcccca aaacgtcagg atcattattg ctgtcggccg
     1501 acaggacgaa cgcaatgatc tgcgtctcgc acttgaagcc ctcggcgaac atgggacgca
     1561 ccggccgatc gatctggcgg ctggtgagca cttcgcgctc gctcatgcgg ccttcgcgct
     1621 tgatgaatcc tccggggaaa cgtccgccgg cgtaggtgta ttcgcggtaa tcaacggtga
     1681 ggggaaagaa atcgatgcct tcgcgcggat cggcgttggc ggtggcgcag gcgagcacga
     1741 cgttttcgcc gaggcggaca acagcggcgc ctccggcctg cttggcgagc tttccagttt
     1801 caaatgagat ttctttgccg ccggatagat ggactgtaac gctagttgct tctggtttca
     1861 tgattcattc ctttgagttg aggcaggcag cgatgagata ggctgctgca cccctccgcc
     1921 aatgcgcgga agggccgggg caccgaatag gtgccgggtt gaggcgaggg atcagcgctt
     1981 gaaaaacgca cgtgtggaga attcctcgat gctggaccgt ctctccggag acggatctca
     2041 gaaagaagca agtttcgaag gtttcgaagg tttcgaaagt tccaagg
//
LOCUS       DATMGX010000908         2480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_236650, whole genome shotgun sequence.
ACCESSION   DATMGX010000908 DATMGX010000000
VERSION     DATMGX010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236650"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VNR65_14845"
     CDS             <1..637
                     /locus_tag="VNR65_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWJ19997.1"
                     /translation="RGSGGHFSAGADLKDMSVARASPIAHHSDPMAETNAKFGHVCAA
                     YAHSPLAIVAVLEGAVMGGAFGLSCAADVAIASHTVDFRLPETSLGVIPAQIAPFLVE
                     RLGYAEAKRLSVTGARFGAGEAHRIGLVHKVCENSVDLEAAVATTVQQILACAPGAIA
                     ETKKLLRRARFEDPAALVEHAAAVFAKAARGPEGEEGMRAFIEKRKPSWAS"
     gene            complement(832..1113)
                     /locus_tag="VNR65_14850"
     CDS             complement(832..1113)
                     /locus_tag="VNR65_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ19998.1"
                     /translation="MQRLALIGVVLFSPLVSTATLAAPSQEQCNDAWNKADKDADGDL
                     SGAELTRYLIAIKKDGRHNEAIKDGKIDVDEFMVVCKDGVFENMEEKHR"
     gene            1339..>2480
                     /locus_tag="VNR65_14855"
     CDS             1339..>2480
                     /locus_tag="VNR65_14855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ19999.1"
                     /translation="MKMKQEAPHPNPAPRKFALASLRLWSFSIRKRMLKAEGAGCSAS
                     MTSQGEAHRATVATRLRSIPAGVWTLGFVSMLMDISSEMIHALLPIYMVSVLGTSTFA
                     VGLIEGIAEATAAITKIFSGALSDWLGRRKELAALGYGLAAFTKPVFPLAPSLPWLVG
                     ARFVDRIGKGIRGAPRDALVADLSPPEIRGASFGLRQSLDTIGAFAGPLLAIILMLAT
                     ANSFTTVFWIAVIPGFLSFFLIAFAVKEPERAGGPKKVKSPLSAAQIARLGPAYWQIV
                     AIAAVFTLARFSEAFLILRAQSVGLPIALVPAVMVLMNVVYAISAYPAGVLSDRVDRY
                     TVLIAGLALLILADAVLALAGGIGGVAVGVILWGLHMGLTQGLLAAL"
BASE COUNT          426 a          768 c          735 g          551 t
ORIGIN      
        1 gcgcggaagc ggcggccatt tctcggcggg cgcggacctc aaggatatgt cggtcgcgcg
       61 cgcctcgccc atcgctcacc acagcgaccc catggccgaa accaacgcca aattcgggca
      121 tgtgtgcgcg gcctatgcgc attcgccgct cgccatcgtc gcggtgcttg agggcgctgt
      181 gatgggcggc gcgttcggac tttcttgcgc cgccgacgtg gccatcgcct cgcacacggt
      241 cgacttccgc ttgccggaaa cgtcgctcgg cgtcatcccc gcgcaaatcg cgccgttcct
      301 ggtggagcgc ctcggctatg cggaggcaaa acggctctca gtgaccggcg ctcgcttcgg
      361 cgcgggcgag gcccaccgca tcgggcttgt tcacaaggtc tgcgaaaact cggtagacct
      421 tgaagctgca gtcgccacga cggtccagca aattcttgcc tgcgctccgg gtgcgattgc
      481 ggaaacgaaa aaacttctgc ggcgcgcgcg ctttgaagat cccgccgccc tcgtcgagca
      541 cgccgccgct gttttcgcca aggcggcgcg cggcccggaa ggcgaagagg gcatgcgcgc
      601 cttcatcgag aaacgcaagc cgtcctgggc ctcgtaggcc ttccaagcag gtcaccgtgg
      661 ctagtcaacg gagggcggat cgcaaggcgc tgcccagcga tctacgcaac agcttgacct
      721 tcaggtgcgg aagtcaataa agcgcaaggc tggccgccac cgcttacaat atcaggctct
      781 gatttgcagc tccgctcatt gatgaaccgc atcgcaacgg tcatcaagac attagcggtg
      841 cttctcctcc atgttctcga aaactccatc tttgcagacg accataaatt cgtccacgtc
      901 gatcttgcca tccttgattg cttcgttgtg tctgccatcc tttttgattg cgatcaaata
      961 gcgtgtgagc tcggcgccgg acaggtcccc gtcagcgtcc ttgtcggcct tgttccaagc
     1021 atcgttgcac tgctcttgcg agggcgcagc aagggttgcg gttgaaacca aaggtgaaaa
     1081 taaaactacc ccaataagcg ctagacgctg catttttgtc tccctcttga ttctgatcgc
     1141 ttataatatc gaaaaccggg tacttgaaaa ggctgcggcg tgagcgtttt gctctttcac
     1201 cgacactcgt cttcgccctc aaaatgcttg taagtggttg atttggtcta gtggaaacgg
     1261 caggtttccc gtcagcatga gacatgccca gaacttctgc aatcggcttt ttgcgcgggt
     1321 tcaaccaggc gttcgggaat gaagatgaag caagaagccc ctcaccccaa ccctgcccct
     1381 cgcaagttcg ccttggcgag cttgcgactt tggagcttca gcatccgcaa gcggatgctg
     1441 aaggcagagg gagcaggctg ctccgcctca atgacgtccc agggtgaggc gcatcgggcc
     1501 acggtggcaa cccggctgcg ctccattccc gctggcgtct ggacgctcgg cttcgtgtcg
     1561 atgctgatgg atatctcgtc cgagatgatc cacgcgcttt tgcccattta catggtgagc
     1621 gtgctcggca cctcgacgtt cgcggttggc ctcatcgaag ggatcgcgga ggccacagcc
     1681 gccattacca agatattctc aggcgcgctg tcggattggc tcggccgccg gaaggaactg
     1741 gccgcgctgg gatatggctt ggcagcgttc acaaagcccg tgtttccgct cgcgccgagc
     1801 ctgccctggc tcgtcggcgc ccgctttgtg gaccggatcg gcaaaggcat ccgaggtgcc
     1861 ccgcgcgatg cgcttgtcgc cgatctctcc ccgcccgaga ttagaggtgc gagctttggc
     1921 ctgcgtcaat cgctcgatac gattggcgct ttcgctgggc cgttgcttgc gatcatcctc
     1981 atgttggcca ccgccaatag tttcaccacg gtattctgga tcgcggtcat tccaggtttt
     2041 ctctctttct ttctgatcgc gtttgctgtg aaggagccgg aaagggcagg agggccgaaa
     2101 aaggtcaagt ctcctctcag cgcagctcaa attgccaggc ttggccccgc ctattggcag
     2161 attgtcgcca tcgcagcggt gttcacgctc gctcgcttca gcgaggcatt cctgatcctg
     2221 cgcgcgcaat cggttgggtt gcccattgcg ctggtccccg ccgtcatggt gctcatgaac
     2281 gtggtctatg cgatctcagc ttaccccgct ggcgtgcttt ccgaccgcgt ggaccgatac
     2341 acggtcctga tcgcgggcct tgctcttctt attctcgcgg atgccgtgct tgctttggcg
     2401 ggcggcattg gcggggtggc agtcggtgtc atcctgtggg ggctgcatat gggactgacc
     2461 cagggcctgc tcgccgctct
//
LOCUS       DATMGX010000909         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_52286, whole genome shotgun sequence.
ACCESSION   DATMGX010000909 DATMGX010000000
VERSION     DATMGX010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52286"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..582
                     /locus_tag="VNR65_14860"
     CDS             118..582
                     /locus_tag="VNR65_14860"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HWJ20000.1"
                     /translation="MKITMRLLAKLGILPLLAILSTGSVSAQTDTGKAILEKLTAKVA
                     KLESACAKDIKKYCRNVTPGEGRMIYCMQAYEDKISTKCAFELEDAATRVLTAADALK
                     DGAIACKAEITGVCGKTQPGQGRIAACLLANKSTASTGCAEAIKKIEGMAAQ"
     gene            complement(666..1706)
                     /locus_tag="VNR65_14865"
                     /pseudo
     CDS             complement(666..1706)
                     /locus_tag="VNR65_14865"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1616..1897)
                     /locus_tag="VNR65_14870"
     CDS             complement(1616..1897)
                     /locus_tag="VNR65_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20001.1"
                     /translation="MAKTATRRRRLSDGYSFAGFRPKETVRGIFGDPDVRIVSLDRRS
                     KKQSAVVAGGSRRAGMIGGYGKFATCRVQDFGLFWNLKCGASRAGVVAP"
BASE COUNT          488 a          621 c          630 g          440 t
ORIGIN      
        1 acgcttgcct tggcacaatg aggatatgat cgaaaaacag ccctccgtct ttttcacgcc
       61 actacggcaa ttccgacgaa gtcgcacggt tcgtagccag tcatcagagg acgttcgatg
      121 aaaattacaa tgcgtttgct ggccaagctc ggaattttgc ccttgctggc tatcttgtcg
      181 acgggctccg tttccgcgca gacggatacc gggaaagcga tcctggaaaa actgactgct
      241 aaagtcgcaa aactcgagag cgcctgcgca aaggatatca agaaatattg ccgtaacgtg
      301 acgcccggag agggccgcat gatttactgc atgcaagctt atgaggacaa gataagcacc
      361 aaatgtgcgt tcgagctcga ggacgccgcg acgcgcgtgc taaccgcggc tgacgcactg
      421 aaagatggtg caattgcatg caaagccgag attaccggcg tgtgcggcaa gacgcagccg
      481 ggccagggcc gcatcgcggc atgtctgtta gcaaacaagt cgacggcttc gactgggtgc
      541 gctgaagcca tcaagaaaat tgaggggatg gccgctcaat aacccgtttg accggttctg
      601 ctcgtgggct caataggtca aagaaaaaag ggtcttcagg gaatcgagtg ggtgattctg
      661 ggggatcata gcggcggcag catgcatgag agaactttga ggcggagata ttcttcgtcg
      721 cgcaatccgt aggcgcgtcg ctggaagacg cggattttgt tgttgaggcc ttcgacgaag
      781 ccgagcgaga ctttgttttc cggtttgcaa taggcggcga taccgtccca gtggcgatca
      841 atcatatcgg cgaagcgttc atagggttcg aggcgctgcc atttgaggct ggcgcgccaa
      901 ttgtcgaaga agcgccgcgc ccagccctcg cgcccgtaat cccagagctg gccgaaagat
      961 tctttcagga ggtaggcggt gttcaaccgc ttgttggcgc tcagcaggag cttgagcgag
     1021 cgcttgccct ccagagtgag attatcgtgg cgcgacagca gcgtgtattt ctggcccttg
     1081 atgaagcgcc gttccttgcc gctgaggcgg gcgtactcgg ccttgcggac cttgtcgagc
     1141 gcttcgccga gatggcgcaa gatgtggaac ttgtcgaaca ggatagcggc ttgcggcgcc
     1201 ttgtccttgg cgacgttgcg gaacggcttc cacatgtcca tcacggcgag cttgatttga
     1261 cggctctttc tgggccccag ccaggcatag aactgcgcca tgctggcctc ggaacgatcc
     1321 gcgccgccga accagatcgg ccgcttgcga accaaatcgc tgaccacgat gcgatagctg
     1381 tggcccttgc ggatcgagat ttcgtcaacg ccgatcgcct tgggccccgg cgtgccggcg
     1441 cgcgcgatct gggccctcat gtactgcatc tccagtgtct tgaccgtgtc ccagtcgagc
     1501 ttcagttcct tggcgacgtc gcggatcgaa gcctgcgcgc agcggcgtcc gacatagaat
     1561 gcaaagcgtt tggtgaagtg cggattgtcc gcgagaaaat ccagtcgctc gcgcttcacg
     1621 gtgccacaac gccggcacgc gatgcgccgc acttcaagtt ccagaacaat ccgaaatcct
     1681 gcacacggca ggtcgcgaac ttgccgtacc cgccgatcat accagcccgt ctggacccgc
     1741 ccgcaacaac cgcagactgt ttttttgagc gccggtcgag gctgacgatc cgtacatccg
     1801 gatcaccgaa aatgccgcgc accgtttcct tcggacggaa acccgcgaac gaatatccat
     1861 ccgacagacg ccgccggcgc gttgctgttt tggccatcgc gtatcatgcc aaattccaac
     1921 accgctccaa gcgcataaaa gctaataccc acaagctgtg ccgccgctat gatccctcag
     1981 aatcacccac tcgattccct gaagacccaa aaaagaagga gatcagctcg tctacacaac
     2041 caagccagca ccatttccga gggacggaaa gatgagtgtg aataccctcg tgtgggaaaa
     2101 ggtaaaatag aaactaaggt tgcgacttca cctatcccgg tgttcattgc gacaataccg
     2161 cgcggaacgg tttagtgca
//
LOCUS       DATMGX010000910         2513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_297560, whole genome shotgun sequence.
ACCESSION   DATMGX010000910 DATMGX010000000
VERSION     DATMGX010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297560"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VNR65_14875"
     CDS             complement(<1..442)
                     /locus_tag="VNR65_14875"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HWJ20002.1"
                     /translation="MAVDPVCGMTVDPQTAKHKAIHQGRTYFFCRSGCKAKFESDPGH
                     YLREDEKSAPCCCTASEAEEDAVTDPVCGMTVNPHTAKYRADYEERPTYFCSAGCKAK
                     FESDPKRYLEPNASALPPVPEGAIYTCPMHPEIRQAGPGSCPICG"
     gene            717..917
                     /locus_tag="VNR65_14880"
     CDS             717..917
                     /locus_tag="VNR65_14880"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWJ20003.1"
                     /translation="MVGDDRYCIDILTQIAAVQAALRKVEGEILQNHVNTCVEHAIAS
                     GNAEEQRRKVAELMEVLNRSIG"
     gene            946..1362
                     /gene="arsC"
                     /locus_tag="VNR65_14885"
     CDS             946..1362
                     /gene="arsC"
                     /locus_tag="VNR65_14885"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065799.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HWJ20004.1"
                     /translation="MSIVIYHNPACGTSRNTLAIIRQSGEEPEVIEYLKTPPSRERLK
                     ELTAAMGIPVRALMREKGTPYHDLGLSDPKWSDDELIDFMIAHPILINRPIVVTPKGT
                     RLCRPSELVLEILPNASIGAFTKEDGEIVKAKGKRG"
     gene            1526..2230
                     /gene="phoU"
                     /locus_tag="VNR65_14890"
     CDS             1526..2230
                     /gene="phoU"
                     /locus_tag="VNR65_14890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWJ20005.1"
                     /translation="MIAHTVTSYDEDLKALDSKILRMGGLAEQQLAAALSAIARADPE
                     EARQAVASDPAIDAMQHEIEDLVVHIIARRQPVAVDLRNVVGAMRIAGDLERIGDMSK
                     NIGKRIASFDETAWLSPMTKSLGIMGNMALMQLKSVLDSYSQRDSALALKVWTQDEEI
                     DRQYNSLFRELLTYMMEDPRTIAHGAHLLFCAKNIERIGDHCTNIAEAVTYMVSGQSI
                     VDIRPKQDALPEDSET"
BASE COUNT          569 a          698 c          754 g          492 t
ORIGIN      
        1 tgccgcaaat cgggcatgac ccaggccctg cctgcctgat ttccgggtgc atcggacagg
       61 tatagatcgc cccctccggc acaggcggca acgcggatgc gttgggctcc agatagcgct
      121 tgggatcact ctcaaacttt gccttgcagc cagcgctgca aaaataagtg gggcgttcct
      181 cgtaatcagc ccgatacttc gccgtatgag ggttgaccgt catgccgcag acgggatcag
      241 tgaccgcgtc ttcttccgcc tccgatgctg tgcagcagca gggcgcgctc ttctcgtctt
      301 ctctcaggta atggccggga tcggattcga acttagcttt gcagccggag cggcaaaaaa
      361 agtacgttcg gccttggtgg atcgctttgt gcttcgcggt ttgcgggtcc acagtcatgc
      421 cgcagacagg gtccacagcc attgagccct ccgtctcgga gccacggtga cgatgtgaat
      481 cgctgccagt gtccatggtg tgctccgctt gttcgctctc atatataccc ccgtagggta
      541 ccttaccgca agaggaccac gaaggcgtca cgagccggag cgaatacagc aggccagctt
      601 ccttgattga tgggcatacc ctctggtggt acaagcgttc catgcagcaa gaggtgaagg
      661 aaaattgcct gcagcggctt gcccggatcg agggccaggt gcgaggcata gcgcgcatgg
      721 ttggggacga ccgttattgt atcgacatcc tcacccagat cgcggcggtt caggccgcgc
      781 tcagaaaggt ggagggtgag atcttgcaga accacgtcaa cacctgcgtc gagcacgcga
      841 tcgcgtctgg aaatgccgag gagcagcgcc gtaaggtggc ggaactcatg gaagtcctca
      901 atcgcagtat aggatgatgg aattgcagga aggcgggttc gaacaatgag catcgttatt
      961 tatcataacc ccgcttgcgg aacctcgcgg aacacgctcg ccatcatccg ccagagcggc
     1021 gaggagccgg aggtcatcga atatttgaaa acgccgccat cgcgggagag gctgaaggaa
     1081 ctgacggccg cgatgggcat tccggtgcgc gcgcttatgc gcgaaaaggg aacgccctac
     1141 cacgatctcg gcctgtcgga cccgaaatgg tcggacgacg agcttataga tttcatgatc
     1201 gcccatccga tcctcatcaa ccgcccgatc gtggtgactc cgaagggcac ccggctctgc
     1261 cgcccgtccg aacttgtgct tgaaatcttg cccaatgcga gtatcggcgc gttcacgaaa
     1321 gaggacggcg agatcgtcaa ggcaaaagga aagcgcggat aagtcaacgc gttcctgggc
     1381 gtcaagccgg cgcgcgaaaa tgttacgcta ttgtcttgtg gatggagtat cgtccgcgcg
     1441 gaaagcttgg cgtgatggag gccacaaggc gtcttgatcg cgcccagtgg gcgcacatat
     1501 ccatttaata aaaaggggac aggacatgat cgcccatacc gtcacctcct atgacgaaga
     1561 cctcaaagct ctggacagca aaatcctgcg aatgggaggc ttggccgagc agcaacttgc
     1621 ggccgcgctg agcgccatag cgcgcgcgga ccctgaagag gcaagacaag ccgtggcgag
     1681 cgatccggcg atcgatgcca tgcagcacga gatcgaggat ctcgtcgtcc acatcatagc
     1741 gaggcggcag ccggtagccg tggatctccg caacgtcgtc ggcgccatgc gcatcgcagg
     1801 cgatctcgaa cgcatcggcg atatgtcgaa gaacattgga aagcggatcg caagcttcga
     1861 cgaaacggct tggctttcgc cgatgacgaa gagtcttggc atcatgggca atatggcctt
     1921 gatgcaactg aaaagcgtgc tcgacagcta ctcgcagcgt gactccgcgt tagcgctcaa
     1981 ggtctggacg caggatgaag aaatcgaccg gcagtataat tctctgttcc gggaactctt
     2041 gacctacatg atggaagatc cgaggaccat cgcccacggt gcgcatctgc ttttttgcgc
     2101 gaaaaacatt gagcgcatag gcgatcactg caccaatatc gccgaggctg tcacctatat
     2161 ggtcagcggc caaagcattg ttgatatccg gccaaaacag gacgctttgc cggaagactc
     2221 ggaaacctaa ttcttcgccg ggtgacgctc gcggtaacgt gacgcataag aaacttaaag
     2281 ggactgaatg aaaagcgagt cttcgttgtc ccaggcgtca aaagataagt ggccggaagc
     2341 ctgtcgaacg catcgctcat cccggaaagg cgaagcctta tccgggattg taaggaattg
     2401 aagcgcctgc ctttttgcga tcccggttcg cgcttcgctt ggccgggatg acggcggcgg
     2461 aaatccgttc gcgaagccga caaaaccagc ttaggtcttg ctgcagctgt tcg
//
LOCUS       DATMGX010000911         3617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_308258, whole genome shotgun sequence.
ACCESSION   DATMGX010000911 DATMGX010000000
VERSION     DATMGX010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308258"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /gene="crcB"
                     /locus_tag="VNR65_14895"
     CDS             complement(<1..793)
                     /gene="crcB"
                     /locus_tag="VNR65_14895"
                     /inference="COORDINATES: protein motif:HMM:NF010802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HWJ20006.1"
                     /translation="MLTYFYIALGGAIGSVARAWTTNVMVRMAGAGFPWGTILINVVG
                     SFIIGFFGALTASDGRFQVHADARAFVMIGICGGYTTFSSFSLQTLDLFRDGKPGAAI
                     ANIGLSVILCLIAVSAGYASAMAFNNGSARTEAYQAPKPAGTRMMGEVALAVLDRPET
                     VGSVLSSAARLLHYGVGGRIEALAVRTPPVAEIMLADQSMSPAEEERFRTHEHDWADA
                     VKATVSRWEQSSAKGVQADFVDAEGDIGHLVAEHGRRSDVVVVPGG"
     regulatory      complement(908..967)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(1944..2696)
                     /locus_tag="VNR65_14900"
     CDS             complement(1944..2696)
                     /locus_tag="VNR65_14900"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ20007.1"
                     /translation="MLPPMFTQATLLHFIIAVALAVHAMKTGRSQWWLFILLLVPLIG
                     SIAYVVIELIPAMFRSQGAKKVATEIGSVLDPDKEWRERVKQAELVDSVDAKRALAEE
                     CEKKGMWADAIRLYETAATGIFADDPAVLTGLARARLGGGDAAAALETLDKLEDVQPR
                     TRNQEAHLLHARALEGLGRTDEALAEYDGVSRYYAGFEAKARYGLLLLKQGRVNQARD
                     MFMEVVRASSAKPVAIAPGDKEWIRIAKANLR"
     gene            complement(2780..3295)
                     /gene="msrA"
                     /locus_tag="VNR65_14905"
     CDS             complement(2780..3295)
                     /gene="msrA"
                     /locus_tag="VNR65_14905"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWJ20008.1"
                     /translation="MFGLGCFWGAEKAFWQLPGVLVTAVGYAGGYTPNPTYEEVCSGR
                     TGHNEVVRVVFDPKRISFGKLLKTFWESHNPTQGMRQGNDAGTQYRSGIYTYSEEQDR
                     EAQASKDAYQSELSARGFGAITTEVVPAQEFYFAEDYHQQYLAKNPRGYCGLGGTGVT
                     CPVGLGAGQTA"
BASE COUNT          699 a         1085 c         1094 g          739 t
ORIGIN      
        1 cgccgccggg gaccacgacc acatccgaac ggcggccgtg ctccgccacg agatgcccga
       61 tgtcgccttc agcgtcgacg aaatcggcct ggacaccttt cgcgctcgat tgctcccatc
      121 ggcttaccgt cgcctttacg gcgtccgccc aatcatgttc gtgggtgcgg aagcgttcct
      181 cttccgcggg gctcatgctt tgatccgcaa gcatgatctc ggcgacaggc ggggtccgga
      241 cggcgagggc ttcgatgcgc ccgccaaccc catagtgcag aagccttgct gcacttgaga
      301 gaacgcttcc cacggtttcg ggacggtcga ggacggcgag ggcaacctct cccatcatcc
      361 ttgtcccggc cggtttcggc gcctggtagg cttcggttcg agctgagccg ttgttgaagg
      421 ccatggcgct cgcatagccg gcgctcacgg caatgaggca aaggatgacg gacaggccga
      481 tgttggcgat cgccgcgccc ggcttgccat cgcgaaaaag atcgagggtc tgcaggctga
      541 aagaggagaa ggtggtgtac ccgccgcaaa tgccgatcat gacgaaggcg cgggcgtcgg
      601 cgtgaacctg gaagcggccg tcgctcgcgg tcagcgcgcc gaagaagccg atgatgaacg
      661 atccaacgac attgatgagg atcgtccccc aggggaatcc tgctccagcc atccggacca
      721 tgacattcgt cgtccaggcg cgcgccacgc tgccgatggc tcctccaagg gcgatgtaga
      781 aataggtcag catcaggtct cccgaacttg aagaggcgaa tgccccggtc gcgtgtccgg
      841 cccttgtttg cacgcgcgca taaaaatacc cgccttgcgg cgggttccgg acagtctccc
      901 gccgccagtt gcagcaggag tcatcagcca aaggcggttt cgggggtact ccatccccat
      961 cgagcgaagt tataaatact cccgtgcgtt ccgcaagccc taagcagtcg tgccaacgac
     1021 cccgaacggt gtgcagtttc tggttcgggg tgattgcatg acggcttaga atcgaagtga
     1081 aaccgcgcgc cagaactagg tctgtccgaa cgaacgaggg ctgctcgata gggcggatac
     1141 tcagcctcgg aagccgctta ctctggctga gggcttcgca cctcatgact ctgagcttca
     1201 agatccaagt cacactgacg tgatccagcc ggagggaata tccaagttgg cgtccaggtc
     1261 tagaggggta tcccagtgag caacaggagc catcgctccc atcgtcaact ggagcagagg
     1321 acagcgaaat gagaactcgc tcaagacctc gttatagatc gacttcgaga atcgcaggag
     1381 accatcatga cactcaatct atctgagaag atcttgacat tgtcagctag cattttgctt
     1441 gttgcagcgt gcggaacgcc ttcggatgca agaccgcata caaattatct caggcctcaa
     1501 atcggaaata tctatgcgaa cgctcctctt gcgccgcgcc aagaaaccag cccctacagg
     1561 gattcgtctt gtttcaatgt ccctgggctg ccggagatgt tcgcgtgcac cccgtacaat
     1621 taggcgtttg gtgacctgag atggagggca gaggctgggc ggtctttgag gagcgcacgc
     1681 ctcttgcgct cgggcgcggc gccattgggc gatgccgctt cggcggctca ggtgaatatg
     1741 ggccgaacgg tgggcggcct gcccggcaat gccgcgtgcc ttcttcagtc tcgtcgaggc
     1801 aacgaggatc gaccgcctcg ttgccggcga tcctgccggt ttcgttgcgg cgcattcggc
     1861 gatcttcgag tcgctaacct tttattctgt tgcgctttcc gggcagaaaa ccgcatacgc
     1921 tttacctgaa agctctagat atctcagcgc agattcgctt tcgcgatcct gatccactcc
     1981 ttgtcgccgg gcgcgatcgc cacgggcttt gcactcgatg cgcgaacaac ctccataaac
     2041 atatcgcgcg cctgattcac gcgcccctgc ttcagaagca gaagcccgta acgtgctttc
     2101 gcctcgaagc ccgcgtaata gcgcgaaacc ccatcatatt ccgccaaagc ctcgtcggtt
     2161 cggcctagcc cttccagcgc gcgtgcgtgg agcagatgag cctcctgatt tcgcgttcgc
     2221 ggctgaacgt cttccagctt gtccagagtt tcgagcgcgg ctgcggcgtc tccgcccccg
     2281 agccgcgcgc gggccagccc tgtaagcacg gcaggatcgt ctgcgaagat cccggttgcg
     2341 gcggtttcgt agagccggat cgcgtcagcc cacatgcctt ttttttcgca ttcctcggcg
     2401 agagcgcgct ttgcatccac cgagtccacg agttcggcct gcttcacacg ttctcgccat
     2461 tccttgtcgg ggtcgagcac ggacccgatc tcggtcgcga cttttttggc cccctgcgac
     2521 ctgaacatcg cagggataag ctcgatcacg acataggcga tagacccgat caagggcacc
     2581 agtagcagga tgaagagcca ccactgcgag cggcctgttt tcatggcgtg cacggccagc
     2641 gccaccgcaa taatgaagtg gagcagggta gcttgagtga acatgggcgg aagcattgac
     2701 ggcaatcctt aaggtatcgt cgcagtgcta accgagaaca tggctgcagg caagctggtg
     2761 caccttgaaa tacagaggtt taggctgtct gccccgctcc gaggccgacg gggcaggtga
     2821 cgccggtgcc tcccaggccg caatagccgc gcgggttctt cgcgagatat tgctggtggt
     2881 aatcttccgc gaagtagaac tcttgagcgg gtacgacctc cgttgtaatc gccccaaagc
     2941 cccgcgcgct cagttcggat tgataggcat ccttcgaggc ctgcgcctcg cggtcctgct
     3001 cctcggaata ggtgtagatg ccggagcggt attgcgttcc ggcgtcattg ccctggcgca
     3061 tgccctgtgt cggattgtgg ctctcccaga acgttttcag aagttttcca aagctgatcc
     3121 gtttgggatc gaagacgacg cgcacgacct cgttgtgacc ggtccggccg gagcacactt
     3181 cctcataggt gggattgggt gtgtatccgc ccgcatagcc gaccgcagtg acgagcacgc
     3241 ccggaagctg ccagaaggcc ttttctgcgc cccagaagca gccaagcccg aacatcgcga
     3301 tctcgctgcc ctccggatac ggacccttga gcggcgtgcc gctcacgaaa tgggttgctg
     3361 cggtccgaat cggggtgtcg cgtccaggca gagcgctttt gggatcgggc atctcggctt
     3421 tgtttttgaa gaagaacgcc atgcagaact cctgtctgtg cggatagccg cttttgacga
     3481 caggatatgg gagttcgggg gccacctcgc caagaaacga gggaaaaggg gcgagacgtt
     3541 cacggagtgt ttatagtgat ttggggggaa ggccgggagt gctcccggcc ttttggatca
     3601 atgggtctcg actttcg
//
LOCUS       DATMGX010000912         3031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_84534, whole genome shotgun sequence.
ACCESSION   DATMGX010000912 DATMGX010000000
VERSION     DATMGX010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84534"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..280)
                     /locus_tag="VNR65_14910"
     CDS             complement(17..280)
                     /locus_tag="VNR65_14910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ20009.1"
                     /translation="MSANGAAPGGHPWTDPSSQWMSAELVPLENDPSLILILSDINMP
                     GRSGLEMLPKVRAERPNVPAIMIAAYGDEDTKRHAIAREPLDC"
     gene            437..1711
                     /gene="oxlT"
                     /locus_tag="VNR65_14915"
     CDS             437..1711
                     /gene="oxlT"
                     /locus_tag="VNR65_14915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HWJ20010.1"
                     /translation="MSIALEAEGRPAATDTTRWSILACGILCMMMIANLQYGWTLFVN
                     PIAAKHGWSIAAVQVAFSIFIALETWLTPIEGWIVDLLGARRGSMIMVAFGGIMVAIG
                     WTMNSYAETLPMLYAAAVFAGIGGGAVYATCVGLAVKWFPDRRGLAVGLIAAGYGAGA
                     AITVIPIRYVIETSGYESAFLWFGLIQGGVVFILAWFLRSPEPGEMADIPAPKIAQSS
                     RSYNPKEVLSSPVFWLLYVLFVMVSASGLMATAQIAPIAKDFDVGNTIVFFGATTLTA
                     ALIIDNVANGSARPLFGWISDHIGREYTMAIAFGLGAAAYWSLGTLGTAPWAFVLFAA
                     LIFFTWGEIFSLFPSTCTDTFGTKFATVNLSLLYTAKGTSAFLVPVANMIKVSTGSWH
                     MVFLLTAIMNILVVALALFVLKPMRARMMTQD"
     gene            1922..2686
                     /locus_tag="VNR65_14920"
     CDS             1922..2686
                     /locus_tag="VNR65_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ20011.1"
                     /translation="MNDPARGNRPVAVDIAVRRDKEMQANVYMITLPVAILNHGNTVR
                     FTEYSFLANVFAAHGYLAISIQHDLSTDAPMVTKVGELYVGRQPQYLRGVANIKFAVE
                     EMKKVQPNADFDRLTLVGHSAGADISVYFAKMYPDHIKAVVTLDNLRVPFMTDGRFKI
                     LSFRSKDPRFKTDPGVIPPRGVRERSGITVVQTGYQHTDMSDRGPDDVKSAIHGMLDK
                     FLDDKSPLRPVVSNAPPLMTDAGPVAPYVWVAPVRH"
BASE COUNT          619 a          859 c          870 g          683 t
ORIGIN      
        1 aaatcgatcg gcttggtcag caatccaacg gctccctggc tattgcgtgc cgcttggtgt
       61 cctcgtcgcc ataggcggct atcataatcg ccggcacgtt cggccgttcg gcccgcacct
      121 ttggcagcat ctcaaggccg ctcctgccgg gcatgttgat gtcggacagg attaggatca
      181 gcgacgggtc gttttccagg ggaactaatt ccgcactcat ccattgtgat gatgggtcgg
      241 tccaaggatg accgccaggc gccgctccat tcgcggacat accttccatc agcgacaaag
      301 ctcccccgcc tcgaaccgac ggcctcacgc tcgcgcgtct cgcgagctgc aggtagctcg
      361 cgaattcgag aaaatgggct ccgtcaagga cgctgcaacc atggaaacgc aatagcgcaa
      421 aacagtagag ggcccgatgt caatcgcact tgaagctgaa ggccggccag cggcgacgga
      481 taccacgcgg tggagcattc tcgcctgcgg catcctttgc atgatgatga tcgccaatct
      541 gcaatatggc tggacacttt ttgtcaatcc cattgcggcc aagcatggct ggtccatcgc
      601 ggcagtccag gtcgccttca gtatttttat cgcgctggaa acctggctta cgccaatcga
      661 aggctggatc gttgacctgt taggtgcacg gcgcggatcg atgatcatgg tcgcgttcgg
      721 tgggattatg gtcgcgattg gttggaccat gaattcctat gcggaaaccc tgccgatgtt
      781 gtacgccgcc gctgtttttg caggtattgg tgggggcgcc gtttatgcca cgtgcgtcgg
      841 gctcgctgta aaatggttcc ccgatcggcg cggtctcgcg gtcggcctga ttgctgcggg
      901 ttacggtgct ggagcggcaa tcaccgtcat tccaattcgt tacgtgatcg aaacaagcgg
      961 atacgaatcg gccttcttat ggtttggttt gattcagggc ggtgtcgtct ttatcttagc
     1021 gtggttcctg cggtcgccgg aaccaggcga aatggccgac attcccgcac cgaagatcgc
     1081 ccaatcgtcc cgcagctata atccgaaaga ggtcttgtct tcgccggtct tctggctgct
     1141 ctacgtcttg ttcgtcatgg tgtccgcgag cggactgatg gcaacagcgc aaatcgcgcc
     1201 gatcgcaaaa gattttgacg ttggaaatac tattgtattt ttcggggcga caaccctgac
     1261 agcggccctg atcatcgata acgtcgcgaa cggatccgcc aggcctctgt tcgggtggat
     1321 ttcggaccat attggtcgcg agtatacgat ggcgatcgcg ttcggattgg gagcagccgc
     1381 ttactggtcg ctggggacgc tgggcacggc tccgtgggcc ttcgtgctct ttgctgcgct
     1441 catcttcttc acctggggcg agattttcag cctctttccg tcaacctgca ccgacacctt
     1501 cggtacgaaa tttgccacgg tgaaccttag cttgctttat acggccaaag gcacatccgc
     1561 attcctggtt ccggtggcca acatgatcaa agtctcgacg ggaagctggc atatggtgtt
     1621 cttgctgacc gccatcatga acattcttgt cgtggcattg gccttgttcg tcctcaagcc
     1681 gatgcgtgcc cggatgatga cacaggactg atcgggaact ctcagggcac gattctctgc
     1741 cctgcgccgg acatatctta gggaaaacgg acagcagacg tcttggcatc tgatccccga
     1801 ggtcaatgca ccgaggacga acatgaaacg tggaattgcc gttctggctt cgcttagcgc
     1861 gttggttacg attgggtact tcaccgcgag caaatggggg atccgtcacg agacgctgac
     1921 gttgaacgat ccggcacgcg gcaaccgtcc ggtcgcggtg gatatcgccg tgcggcgcga
     1981 caaggaaatg caggcgaacg tctacatgat cacgctgccg gtggcgatcc tcaaccatgg
     2041 caacacagtc agattcaccg agtattcttt tctggcgaac gttttcgccg cgcacggtta
     2101 tctggcaatc agcatccagc atgatctgtc aaccgatgcg cccatggtga cgaaggtcgg
     2161 cgagctctat gtaggccgtc agccgcagta cctgcgcgga gtcgccaaca taaaatttgc
     2221 cgtcgaggaa atgaaaaagg tccagccgaa cgcggatttt gaccgcctga ccttggtggg
     2281 acattccgct ggcgccgaca tctcggtgta tttcgccaag atgtatcccg atcatatcaa
     2341 agcggtcgtg acgctcgata acctgcgcgt gccgttcatg actgacggca gattcaagat
     2401 cctgtctttc cgttcgaagg atccgcggtt caagaccgat cccggggtca ttcctcccag
     2461 aggggtccgt gagaggtctg gcattacggt cgtacaaacc ggctaccagc acaccgacat
     2521 gagcgatcgc ggtcccgacg atgttaaatc ggcgatccat ggcatgctcg acaaattcct
     2581 cgatgacaaa agcccgctca ggccggtcgt ttcaaacgcg ccgccgctga tgaccgatgc
     2641 cggtccggtc gcgccctacg tgtgggtcgc accggtccgg cactgaggcc ggcatcacaa
     2701 gcttcgaatc gaagcaaaca taccagccgt gagtaggatt ggcgtgtcta taacacaata
     2761 tgatgcgcca ctagagacgt tgggcaattg cactagtcgg cctcagactt ggatccagcc
     2821 gatgtccgaa acgggtcaaa aatgcgaaat actcatcccg agtagatgtc ttccgctttg
     2881 cccccgacaa cagatgaggt ggatggctcc cgctcacggc atcttggtgc caaggtgtga
     2941 aggccatcag gcgttcacga gcgaaaaggg agccatccgc tatgcagatt accacgatcg
     3001 gcctcgactt ggccaagagc gtttttcagg t
//
LOCUS       DATMGX010000913         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_164537, whole genome shotgun sequence.
ACCESSION   DATMGX010000913 DATMGX010000000
VERSION     DATMGX010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164537"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2066)
                     /locus_tag="VNR65_14925"
     CDS             complement(<1..>2066)
                     /locus_tag="VNR65_14925"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ20012.1"
                     /translation="LTPDGRLKILDFGLAQLEPHASELGLTATLTKSQEITGTLPYMS
                     PEQLRGERADARSDIWSAGAVLYEMANGKRPFPQSNQAMLINAILNVPPEPPSKKITG
                     PVLMGAEQIILKCLDKDPGRRYQSARELAVDLERLSTGTLPVGVPQSAPTNRYFLAGV
                     VACLLVALTVGYFVINRKQRSAPATAPPSHRRSIAVLGFQNLAGKAEDAWLSTALSEM
                     LTTELAQGDQLRTIPGESVAQMKRSLSLPDADSFSQDTLSRIHENLGTDDVVIGSYVP
                     LAAGVIRVDLRLQETKAGEILASVSEKGSASEIDQLVSKAGAELRAKLGVSALSDAES
                     ASVRAALPSVPEAARLYSEGLKKLRLFDALEARALLERAAALDPNHAPTHSALAEALS
                     NLGYDEKAKQEAKRALDLASSSPREERLLIDGRYRSLTKDWSGAMSSYRTLWGFFPDR
                     IDYGLLLARAQTSNDQPNEALATIAELRELSLSKGESALIDLAESSVAAARSDYKLQQ
                     SSAEKAAQAGAAIGANLLVAGALRMEGNAWQRLSQPEKSLAALERAQELYTAAGDRNG
                     AAICLMLAGDNTYNQGQFADARKQFERALPVFREIGDLHHTRGTLERIGNTFYDQGNL
                     HVAIDFYQQALKIDLATGQTSDMASDYGNIANALDGLGDLTGSRKMQEQALAAFEKTS
                     DRRGAA"
BASE COUNT          396 a          552 c          680 g          438 t
ORIGIN      
        1 tgccgcgcca cggcgatcgc tggtcttttc gaaggcggcg agagcctgtt cttgcatttt
       61 gcgggaaccc gtcagatccc cgagaccgtc gagggcgttg gcgatattgc cgtagtcgct
      121 ggccatgtca ctggtttgcc cggtcgcgag atcgatcttc agcgcctgtt ggtaaaagtc
      181 gatagcgacg tgtaaatttc cctggtcata gaatgtattg ccgattcttt cgagagttcc
      241 gcgggtatga tgcaggtccc cgatctctcg aaaaactggg agcgcccgtt cgaactgttt
      301 tctcgcgtcg gcaaactgtc cctggttata ggtgttgtcg ccggccaaca tcaggcaaat
      361 ggcggctccg ttgcggtcgc cggcggcggt gtaaagctcc tgtgcgcgct cgagcgctgc
      421 cagggatttt tccggctgac tcaagcgctg ccaggcgttg ccttccatac gcaaggcccc
      481 ggcaaccagg agatttgcgc caatggcggc tccggcctgc gctgctttct ctgctgacga
      541 ctgttgcagt ttgtagtcgc tgcgagcagc ggcgaccgag gactcggcga ggtcgatgag
      601 ggcgctctcg cctttggaaa gagacagctc gcgaagttca gcgatggtgg caagagcttc
      661 gttgggctga tcgtttgagg tttgggcccg cgcgagaagc aagccgtagt ctatgcggtc
      721 ggggaagaat ccccacaagg tgcggtagct actcatggcg ccggaccagt ctttggtgag
      781 agaccggtag cggccgtcga tcaagagccg ttcctcgcgt ggggaagaag aagctaaatc
      841 gagggcgcgc ttcgcttctt gtttggcttt ctcgtcgtag ccaagattgg acagggcctc
      901 agccagtgcg gagtgcgtgg gagcgtgatt gggatcaagg gcggcggctc gttccagcag
      961 agcacgcgcc tccagagcat caaacagacg gagtttttta agaccctcgg aatagaggcg
     1021 ggcggcttcg ggaaccgagg gcagagccgc ccggacactg gccgactcag cgtcggaaag
     1081 tgcagagaca ccgagcttcg cccgcaactc ggcgcctgct ttgctgacca gttggtcaat
     1141 ttcggaggca cttcctttct cggaaacgga cgcgagaatt tcgcccgcct tggtctcctg
     1201 cagacggaga tccacgcgga tgacaccggc ggccagcgga acgtaagaac cgattacgac
     1261 gtcatcagtg cccagatttt cgtggatgcg ggatagcgtg tcttggctaa agctatcggc
     1321 atcgggcaag gagagactgc gtttcatttg cgcaacactt tcgccgggaa tggtgcgcaa
     1381 ttgatctcct tgggcaagct cggtggtgag catttccgag agtgcggtgg aaagccaggc
     1441 gtcttcggcc ttaccggcaa gattctggaa tccaagcacg gcgattgagc ggcggtggct
     1501 gggcggtgct gtcgcaggag ccgaacgttg cttccgattg atgacgaaat agccaacggt
     1561 caaggcaacc aggagacatg cgactacgcc ggcaaggaag taccgattcg tgggggcgga
     1621 ttgaggaaca ccgacgggta aagtgccagt gctcaggcgt tccaggtcaa cggcaagttc
     1681 gcgcgcgctc tggtagcgcc ggccaggatc cttgtccaga cacttcagga tgatttgttc
     1741 cgcacccatt aaaacggggc cggtgatttt cttactgggc ggctccggag gcacattcaa
     1801 aatggcattg atgagcattg cctggttgga ttgcgggaaa gggcgtttgc cgtttgccat
     1861 ttcatacagc acggctccgg cggaccagat gtcgctgcgc gcatcggccc gctctccgcg
     1921 cagctgctcg ggcgacatat aagggagagt cccggtgatt tcctgcgatt tggttaaggt
     1981 ggcggtcaag cccagctcgc tggcatgggg ctcaagttgc gccaaaccga agtcgaggat
     2041 tttgaggcgg ccgtcaggag tgaggc
//
LOCUS       DATMGX010000914         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_180008, whole genome shotgun sequence.
ACCESSION   DATMGX010000914 DATMGX010000000
VERSION     DATMGX010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180008"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..>2274)
                     /gene="phaC"
                     /locus_tag="VNR65_14930"
     CDS             complement(359..>2274)
                     /gene="phaC"
                     /locus_tag="VNR65_14930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HWJ20013.1"
                     /translation="VFNQYLENTEDEDGFRIMHPAIVSRAFQDMAIKALEHPAEIVKE
                     QIDYWNSMAELWQRGMAKFLFNKEFEPVATPAPADRRFKDEAWAENVVFDCIKQCYLL
                     SARHLQSAVSQVHGLDEHTTKKVQFYTRQFIDAISPTNFAATNPEVLKATIESKGENL
                     LEGVAHMLQDLDRNHGNFNVKMTNLEHFHLGENLATTPGKVIYQNEMMQLLQYNPSTE
                     TVRQRPLLVVPAWINRFYILDLQPKNSFVKWAVDQGHTVFLISWVNPDESYADKTFDE
                     YLIKGPIAALDAIEQATGEKEVNAVGYCIGGTLLGITAAYMAAKRDKRIVSCTYFTSM
                     FDFSEPGEIGVFIDEAQIDLLEKHMRFKGVLEGHHLAEAFSMLRENDLIWSFFVKDYL
                     LGQEPMPFDILYWNSDSTNMPAAMHGFYVRNMYLHNRLREPNGITLAGEGIDLHRITI
                     PQYFLSASEDHIAPWKSTYAGTQLVSGPVRFVLGASGHIAGVMNNPAAKKYCYWTKDK
                     IAATPDEWLAGAERHEGSWWGDWDQWVGQLAGESVPARQPGDGALTPIEDAPGSYVKE
                     LHTLDKPEQAFVERVQGALQSLDVPTKQDIDTLSEQVHALAKVANRLSAQLGETQSGA
                     KTKTRRTSRRAHR"
BASE COUNT          419 a          674 c          657 g          524 t
ORIGIN      
        1 caggaaggca tcgcggtgcg ttcgggccat cactgcgcgc agccgatcct gcgccggttc
       61 gggctggaaa gcaccgtgcg cccgtcgttt gcgctttata acacctacgc cgacatcgac
      121 gcgctggcgg cggctttgcg ccggatccag agttgccata atccctatat gagctaagaa
      181 atggataggc tcagtgctta ttgcgccggg ctttcgccaa tcatcaccgc tgattttgcg
      241 gtaccactta accaaccgcc gcagcatttc gtatggccga gcgggccccc ctgcaagggg
      301 gacgcccgac gagggattgc gtttgcctgg tctttgacca ccccttggtg cgcatggctt
      361 acctatgagc gcgtcttgac gtcctcctgg tctttgtttt ggcgccggat tgcgtctctc
      421 cgagctgagc cgagagcctg ttcgccacct tggccaacgc gtgaacctgc tccgacagcg
      481 tatcaatatc ttgtttcgtc gggacatcga ggctttgcaa ggcgccctgc acgcgctcaa
      541 caaaggcttg ctcaggcttg tccaaggtgt gcaactcttt cacgtaagaa cctggggcat
      601 cttcgatcgg cgtgagcgca ccgtctccgg gttggcgggc aggcacagac tctccggcta
      661 actgaccgac ccattgatcc caatcccccc accacgatcc ttcgtggcgc tctgccccgg
      721 caagccactc gtccggagtg gcggcaatct tgtctttcgt ccaataacaa tatttcttgg
      781 cggccgggtt gttcatcact ccggcgatat ggcccgaggc acccaggacg aatcgcactg
      841 gaccggacac aagctgagtt ccggcatagg tcgatttcca cggagcgatg tggtcctcgc
      901 tggccgacag gaagtattgg ggaatcgtga tgcggtgaag atcgatgcct tctcccgcga
      961 gcgtgatgcc gttcggttca cgcaaacgat tgtggaggta catgttgcgc acatagaagc
     1021 cgtgcatggc cgcgggcatg ttggtcgagt ccgagttcca gtacaggatg tcgaacggca
     1081 tcggctcctg gcccaggaga tagtccttga cgaagaacga ccagataagg tcgttctcgc
     1141 gcagcatgct gaaagcctcg gccagatgat ggccctcgag gacccccttg aaccgcatgt
     1201 gcttctccaa gaggtcgatc tgcgcctcgt cgatgaagac gccgatttct cccggctcgg
     1261 agaagtcgaa catcgacgtg aaataggtgc agctgacgat ccgtttgtcc cgcttcgccg
     1321 ccatgtacgc cgcggtgatg cccaacagcg ttccgccgat gcaatagccg acggcgttga
     1381 cctccttttc acccgtcgcc tgctcgatcg catcgagcgc ggcaatcgga cccttgatga
     1441 ggtattcgtc gaaggtcttg tcggcatagc tttcgtccgg gttgacccat gatatgagga
     1501 acaccgtatg gccctggtcg accgcccact tgacgaacga attcttgggc tgcaagtcga
     1561 ggatatagaa ccggttgatc caggccggca caaccaacag cggccgctgg cgcaccgtct
     1621 cggtcgacgg attgtattgc agcagttgca tcatctcatt ctggtagatg accttgcccg
     1681 gcgtcgtcgc gagattctcg ccgaggtgga agtgttccag attggtcatc ttgacattga
     1741 agttgccgtg gttgcgatcc aggtcctgca gcatgtgcgc gacgccctcc aaaaggttct
     1801 cgcccttgct ctcgatcgtc gccttgagga cttcgggatt ggtcgccgcg aagttggtgg
     1861 gcgatatggc gtcaatgaat tggcgggtat agaactgcac cttcttggtc gtgtgctcgt
     1921 cgagcccgtg gacttggctg accgctgatt gcaggtgccg ggcgctcaaa aggtagcact
     1981 gcttgatgca gtcgaaaacg acgttctcgg cccacgcttc gtccttgaag cggcggtcgg
     2041 ccggcgccgg ggtcgccacc ggttcgaatt ccttgttgaa cagaaacttg gccatgcccc
     2101 tttgccacag ctcggccatg ctattccaat aatctatctg ctctttgacg atctcggccg
     2161 ggtgttccag cgctttgatc gccatgtcct ggaaggccct gcttacgatt gccggatgca
     2221 tgatcctgaa tccatcttca tcttcagtat tctcgaggta ctgattgaac acat
//
LOCUS       DATMGX010000915         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_270292, whole genome shotgun sequence.
ACCESSION   DATMGX010000915 DATMGX010000000
VERSION     DATMGX010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270292"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VNR65_14935"
     CDS             complement(<1..299)
                     /locus_tag="VNR65_14935"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWJ20014.1"
                     /translation="MATNQSTVDYIVEQIAGAGAVAGRKMFGEYALFCDGKMVALICD
                     DQLYIKPTVAGRSHLSVVIEKPPYKGAKPYFWISGDRWDDSDWLTELVRLSAAELP"
     gene            complement(301..846)
                     /locus_tag="VNR65_14940"
     CDS             complement(301..846)
                     /locus_tag="VNR65_14940"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ20015.1"
                     /translation="MKLLDGSSHEPLALVGSRTTHWMRTTIDSELTNKVSWTRLRVII
                     IQERPMKLGYVILYVESVADTVAFYERAFGLERGMVTPTNEYGELKTGDTKLAFAANA
                     FVKTLTAVPFEVASPAKAAPPVELGLVTQEVEAAFAKAVAAGAVAVKQPEKKPWGQLV
                     GYVRDNNGFLVEICSPFGYLG"
     gene            complement(901..1176)
                     /locus_tag="VNR65_14945"
     CDS             complement(901..1176)
                     /locus_tag="VNR65_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20016.1"
                     /translation="MQLHTIDAGNLAFQCVAFAPEITGQPCQGAAERDVEFAHHAGLV
                     DEARQPPPRVGLAIQISQVARQQGAPGVDYGSGQVVLGLEVVEYIAR"
     gene            complement(1210..2193)
                     /locus_tag="VNR65_14950"
     CDS             complement(1210..2193)
                     /locus_tag="VNR65_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20017.1"
                     /translation="MAADVFNDGDGAKSYRIDGLHDVRYIEIYLVGKDPKTGKLVGEC
                     YNSSLLPGGIPASKDTSPQASVEGLDFDKMKTKYGVLSTSLNGPKLWMLDWVDIEVGV
                     TRDFNGIKAPWVAALNMETDADVSKIPAYKPVTIARKSAAGWNKGTMALLLAVVDGEA
                     VKSTSPFVARDYPRCRSCDGDTVPNRGPFHYVRVAVSCSSAQSGAGYGEFHVITICVA
                     SVQRTHDTGTTPNGNDRFSPAVVKGASALRVRFREPPHWPGAAVTGAKRQSLLQNSRF
                     MHEPISNLPQPLLEPCRLLVDERGIRLRVSQQYRAMIPGHQLARIFLRNGR"
BASE COUNT          473 a          746 c          771 g          547 t
ORIGIN      
        1 ggcaactccg ccgccgaaag ccgaaccagt tcggtcagcc agtcgctatc atcccatcga
       61 tcgcctgaaa tccagaagta gggcttcgct cccttgtagg gcggcttctc gatgacgacg
      121 cttaggtgag agcggccagc aacagtcggt ttgatgtaaa gctggtcatc gcagatcagc
      181 gcaaccattt tgccgtcgca gaacagggca tactcgccga acatcttgcg ccccgcgacg
      241 gctcccgcgc ccgcgatctg ctcgacgatg taatccacgg tgctttggtt cgtagccatc
      301 tcagcctagg tatccaaagg gagagcaaat ttcaacgagg aacccgttgt tgtcgcggac
      361 gtatccgacg agctgacccc aaggcttttt ctccggttgc ttgaccgcga ccgcaccggc
      421 ggcgaccgcc ttggcaaagg cggcttcgac ttcctgggtg accaggccga gttcgactgg
      481 cggcgctgct ttcgcgggac tcgctacttc gaatggcacg gcggtgagcg tcttgacgaa
      541 agcatttgcc gcgaaggcga gtttcgtgtc cccggttttc agctcgccat actcgttggt
      601 cggcgtcacc atccctcgct caaggccgaa cgccctttcg tagaaggcga ccgtgtcagc
      661 tacggattcc acatacagga tcacatagcc gagcttcatg ggtctctcct gaatgatgat
      721 gacgcgcagc ctagtccatg atactttgtt ggtcaactca ctatctatgg tggtgcgcat
      781 ccaatgggtc gtccggctac cgacaagcgc caaaggctcg tgactgctgc catcgagcag
      841 tttcatcaga atggatatgc ccggacctcc cttgccgatg tggcaaaggc agcgggccta
      901 tcagcgggca atgtattcta ctacttcaag gccaaggacg acctggccag agccgtaatc
      961 gacgcctggt gcgccctgtt ggcgggctac ctggctgatc tggattgcga gaccgacccg
     1021 tggcggcggc tggcgggctt catcaaccag gcccgcgtga tgcgcgaatt ctacgtcacg
     1081 ttcggctgcc ccttggcagg gctgacccgt gatctccggc gcgaaggcga cgcactgaaa
     1141 ggcgaggttg cccgcatcta tggtgtgcag ctgcaatggc tggccacgca gttcgcgcag
     1201 ggcggtgcct catcggccgt tgcggaggaa tattcgcgcg agctgatgac ccggtatcat
     1261 ggcgcgatac tgctggctta cacgcaagcg gatacctcgt tcatcgacca ggaggcggca
     1321 cggctcaaga agtggttgcg gcaagttcga gatgggttcg tgcataaatc ttgaattctg
     1381 caacagagat tgccgcttag cgcccgttac ggccgctcca ggccaatgtg gtggttctcg
     1441 aaaacggacg cgaagtgctg aggcaccttt tacgaccgcg ggtgaaaacc ggtcgttgcc
     1501 gttgggcgtc gttccagtgt catgcgtccg ttggacactg gcgacacata tcgtgatgac
     1561 atgaaattca ccatatccgg ctccggattg cgcggagctg cagctgacag ctacgcggac
     1621 gtagtgaaag gggccgcgat tggggacagt atcaccgtcg caagacctgc atcttggata
     1681 atcacgagca acaaacggtg acgtggattt caccgcttcg ccgtctacaa ctgcaaggag
     1741 cagcgccatc gtgcccttgt tccaacccgc ggcgctcttc cgtgcgatgg tcacgggctt
     1801 gtaggccggg attttgctca cgtcggcgtc ggtttccatg ttcagcgcgg cgacccacgg
     1861 ggccttgatg ccgttgaagt cccttgtgac tccaacttcg atatccaccc aatcgagcat
     1921 ccagagtttc gggccattca gcgacgtgct aaggacgccg tatttggtct tcatcttgtc
     1981 gaaatcgagg ccctcgaccg acgcctgagg ggaggtgtcc ttggaagccg gaatgccccc
     2041 gggcagcaag gacgagttgt aacattcgcc gaccagtttc ccggtcttcg ggtctttgcc
     2101 cacgaggtag atctcgatgt aacgcacatc gtgcaggccg tcgatccggt aggattttgc
     2161 gccgtcgccg tcgttgaaca cgtcggcggc catgctggcg tcgcaggtga aggcaagtcc
     2221 tgctgccagc agcgcgacgc cgaagatgct cttgtatgtg ttcgtcgcaa tgttttgctc
     2281 cggttcatgg tcatcggttt aacaggggtg agttcttagt tggcctttgt gagaggccgg
     2341 cggcccgcgt ggttcacagg cgaatcaatt tatttcgcca gctcgatatc gccgggccgc
     2401 caggtctggt tgaaccacgg ttccagcggg ccatagaggc gcagggcgac gttgtagccc
     2461 gtgccgggca tggtctcgcg cccagttctt ctcctggccc gccggcgcct tggggccgaa
     2521 ccagatggtg acgccgc
//
LOCUS       DATMGX010000916         2850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_436286, whole genome shotgun sequence.
ACCESSION   DATMGX010000916 DATMGX010000000
VERSION     DATMGX010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436286"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VNR65_14955"
     CDS             <1..241
                     /locus_tag="VNR65_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HWJ20018.1"
                     /translation="PRGFAHGFVVISDCADFFYKCDAPYSAKDEIVLQWDDPQLGIDW
                     GNTAPIVSQRDKNGRSLAELEPQLPRYEPSGCASS"
     gene            226..1125
                     /gene="rfbD"
                     /locus_tag="VNR65_14960"
     CDS             226..1125
                     /gene="rfbD"
                     /locus_tag="VNR65_14960"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HWJ20019.1"
                     /translation="MRVLVTGVTGQVGGALVARLKGLGTIVPANRERFDLSRPEALSR
                     ALDEIAPDLIINPAAYTAVDRAEHEYELALQVNGEAPGAMARWAAAHGIPLVHFSTDY
                     VFDGSGERPWVEDACPRPLSAYGASKLAGEDLVREAGGSSLIVRTSWVYAASGTNFLR
                     SIARLARERDELRIVADQVGAPTSAALIADAVAKIVAEGGEHLRKNFADANGLVHLAA
                     SGETSWYDFASAIVVGLRSRGVPLAVKRILPIRTEQYPTPATRPKNSRLDLGRLNKVF
                     GIAPPPWDAALAVELDRLAQELA"
     gene            complement(1475..1678)
                     /locus_tag="VNR65_14965"
     CDS             complement(1475..1678)
                     /locus_tag="VNR65_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20020.1"
                     /translation="MPALVVLNEDAVPSIYWQRSEPRLKRQNLADELKNGADIEGVAL
                     SDLERVLSVVMGFSAKQVQALRR"
     gene            complement(1732..2040)
                     /locus_tag="VNR65_14970"
     CDS             complement(1732..2040)
                     /locus_tag="VNR65_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20021.1"
                     /translation="MLANSTTFECRVGVFGFAAATYRAIRDRIRAQDPQIEEQTLADT
                     VEGITDFHEIVQAIIRAAVADEALARGLKCRLSDLHGRLDRLEERAAKRRQIAKDAMV
                     "
     gene            complement(2091..2212)
                     /locus_tag="VNR65_14975"
                     /pseudo
     CDS             complement(2091..2212)
                     /locus_tag="VNR65_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611857.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2302..>2850)
                     /locus_tag="VNR65_14980"
     CDS             complement(2302..>2850)
                     /locus_tag="VNR65_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20022.1"
                     /translation="AAILIRRFRLGPWGYSAIASIALVVAVGIVVPLHGFQKVDLTLA
                     FAARAPAPLIALTQRTLTETAWWGTGAGTYAAALPIYRGISELTAGPLAPTAAANIAV
                     ELGRPFFWAVLAAAMALFITFLSRALKRHRDSFYSIAGAGCIMTISLLAFTNISVLST
                     PVLVVTSAVIGLAIAQSKSRSA"
BASE COUNT          571 a          842 c          858 g          579 t
ORIGIN      
        1 gccgcgcggc tttgcccatg gcttcgtggt gatctccgac tgcgccgatt ttttctacaa
       61 gtgcgatgct ccatacagcg ccaaagacga gatcgttcta caatgggatg acccccaact
      121 tgggatcgat tggggcaata cggcgccgat cgtgtcacag cgggacaaga acggccgcag
      181 cctcgccgag ctggagcctc agttaccgcg atacgagcct tctggatgcg cgtcctcgta
      241 accggtgtca cggggcaggt gggcggcgcg ttggtcgcgc ggctcaaagg actgggcacc
      301 attgtgccgg cgaaccgcga gaggttcgat ctcagcaggc cggaagctct ctcacgcgcc
      361 ttggacgaaa ttgcgccaga tcttatcatc aaccccgctg cctacacggc ggtcgacagg
      421 gctgaacatg aatacgagct ggccctgcag gtgaacggcg aggcaccggg tgcaatggcg
      481 cgctgggccg cggcgcacgg catacccctg gtccatttct caaccgacta tgtgtttgac
      541 ggcagtggcg agcggccatg ggtggaggac gcttgcccgc ggccactgtc cgcttacggt
      601 gcgagcaaac tcgccggaga agatttagtt cgcgaagcgg gcggctcgtc gctcattgtg
      661 cgcacatcgt gggtctacgc cgcgtccgga acgaacttcc tgcgatctat cgcgcgtctg
      721 gcccgcgagc gagacgaatt gcgcattgtg gccgaccagg ttggggctcc cacctccgct
      781 gcgcttattg cagacgccgt cgctaagatc gtcgctgagg gaggggagca tctacgcaaa
      841 aactttgctg acgcaaatgg tctcgtgcat ctggcagcct ccggtgagac cagttggtat
      901 gatttcgcgt cagccatcgt agtcggcttg cgatcccgcg gtgtcccgct tgccgtcaag
      961 cgcattctgc caattcggac ggaacaatat ccaaccccgg cgacgcgacc aaaaaactcg
     1021 cggctggacc tcggtcgcct caataaggtt ttcggcattg cgccgccgcc atgggacgca
     1081 gcgcttgccg ttgaactgga ccggctggcg caggagcttg cttaagagct atcctgggaa
     1141 catcacgtgg aattacaggg cttgcaacgt gtgaatagac tgatgcgtgc gccgcttccg
     1201 ccttattgcc cgcggctcaa tgtgtttggc taccaaggcc ctcccggatg atggacgcgc
     1261 cgtcagtgtg tacggtgacc cttacgcagg cgaggtaaac cgccaggaaa ttcccgcggt
     1321 tttcgcgggc caacacacag cgggattcga ccgtctcccg gttccaacta tcaaagccga
     1381 agattcggtt ggccttcgaa atcgcttacc agccctcgag gtaggtcagt tcgcggccgt
     1441 gggcctcgcg ggtccggata tagcggctat tggactaacg ccgcagcgcc tgaacctgtt
     1501 ttgctgaaaa gcccattacc acgctcagga cccgttcgag gtcggatagg gcgacgcctt
     1561 cgatatctgc cccattttta agctcgtcgg ccaaattctg gcgcttaaga cggggctcac
     1621 tccgctgcca atagatactt gggacggcat cctcgtttaa caccaccagg gcgggcattc
     1681 cgggccgtat agtcgcggaa gggtcgggcg ccgttatttc ttcaggtcga actaaaccat
     1741 cgcgtccttg gcgatctggc ggcgtttggc ggcccgttct tccaggcggt ctagtcggcc
     1801 gtgcaagtcc gaaagccgac acttgagccc cctggccagg gcctcgtctg ccacggcggc
     1861 tcggataatc gcctggacga tttcatggaa gtcggtgatg ccctcgaccg tatcggcgag
     1921 ggtctgctcc tcgatctggg ggtcttgcgc ccggatacgg tcgcggatgg cgcgataggt
     1981 agctgcagcg aagccaaaaa cacctactcg acattcgaag gtcgtactgt ttgccagcac
     2041 gagtatattg actttcgtac cgttactatt tgttcccggc gttgctcggc aatccggttc
     2101 cagacctttg gcggcgccga tggtttatct tcaaggccat cgaagccatg ttcctgatag
     2161 cgatcgtacc actggtagaa ggcgatcttg gaatgcccaa atgctccagc gtcagacaac
     2221 ccgaaattct ggtcgatgtt tagcgtacca cgcgctgcgc gagtggcagt gaatagccgc
     2281 ttgtggactc atggttctaa ttcaagccga acggcttttg ctctgtgcaa tcgcgagacc
     2341 aatgacggcc gaggtgacca ccaaaacagg cgtgctcaac acgcttatat tggtaaacgc
     2401 cagtagcgag atggtcatga tacaacctgc tccggctatc gaatagaatg aatctctgtg
     2461 gcgcttcagc gcgcgggaca agaaagtgat gaagagtgcc atcgctgccg ccaaaaccgc
     2521 ccagaaaaac ggccgaccca actcaacggc tatatttgcg gcggcagtcg gcgctaaagg
     2581 ccccgcggtt agttcgctga tgcctcggta aataggcaga gcggcggcgt atgtccctgc
     2641 accagtcccc caccaggccg tttccgtcag cgtgcgttgg gtgagggcaa tcagtggtgc
     2701 gggagcacgg gctgcaaaag cgagcgttaa gtccaccttt tgaaagccgt gcagggggac
     2761 aacgatgcca accgcaacaa ccaacgcaat tgacgcgatt gccgagtatc cccacggccc
     2821 gaggcgaaaa cggcggatca agatcgccgc
//
LOCUS       DATMGX010000917         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500 k141_9836,
            whole genome shotgun sequence.
ACCESSION   DATMGX010000917 DATMGX010000000
VERSION     DATMGX010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9836"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VNR65_14985"
     CDS             <1..1094
                     /locus_tag="VNR65_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWJ20023.1"
                     /translation="GPTIGGYLTDLFSWHWLFLVNVVPGIVVTVVSWFLIDFDKPNFK
                     LLRNFDWIGFLTMAGFLGALEYILEEGPTDNWFDSDHITMGVIFSATCAIFFFWRVLS
                     AEHPVVDLSAFKDRNFATGSLFSFVLGVGLYGLTYLYPVYLARVRGYSALQVGETMFV
                     TGICMFLMAPISGKLMQRGVDPRLMLALGFTGLAAGTWQVSLMTVDWDFYELLVPQIL
                     RGVSLMLIMIPVTTTALGTTPPWKLKNASGLFNLTRNLGGAVGLALINTLLNKRLDLH
                     LARLHEQVTWGRAAAEEALAKLTEQMAHFGSDASLAATKKLALLVRRQATVMAIADVF
                     WALTVLFLLIALLAPIVRRPKAQGGGGGH"
     gene            2337..>2897
                     /locus_tag="VNR65_14990"
     CDS             2337..>2897
                     /locus_tag="VNR65_14990"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HWJ20024.1"
                     /translation="MSVLIAFEESSFICAMMNWDRLDFRRLGSAAALAFRLTAAALLA
                     LWLAHRLKVALPLWSVLTALIVTQISLGRSLKATLDYFAGTIGGVVWGACVALLVPHT
                     TEASLLLVLLLALAPLAFTAALHPWLSAGPITAAIVVLLPQMMHTSPIDSAIERVIEV
                     LLGGVAGLFVSFVLVPWSAFQHTREVA"
BASE COUNT          552 a          847 c          827 g          671 t
ORIGIN      
        1 tcggaccaac gatcggcggc tatctcaccg atctcttctc ctggcattgg ctgtttctcg
       61 tcaacgtggt gccggggatc gtggtgacgg tggtttcctg gttcctgatc gatttcgaca
      121 agcccaattt caagctcctg cgcaacttcg actggatcgg cttcctgacg atggcgggct
      181 tcctcggcgc gctcgaatac atcctggaag aagggccgac cgacaactgg ttcgacagcg
      241 atcacatcac catgggcgtc atcttctccg ccacatgcgc catcttcttc ttctggcgcg
      301 tgctctcggc ggaacacccg gttgtcgatc tgtcggcttt caaggatcgc aatttcgcga
      361 ccgggtcgct gttttccttc gtgctcggcg tcggccttta cgggctgacc tatctttacc
      421 ccgtttatct cgcccgcgtg cgcggttatt ccgccttgca ggtcggcgag accatgttcg
      481 tcaccggcat ttgcatgttc ttgatggcgc cgatctcggg caagctgatg cagcgaggcg
      541 tggacccgcg cctcatgctt gcgctaggct tcacgggcct cgcggccggc acctggcaag
      601 tctcgctcat gaccgtggat tgggacttct acgaactcct ggtgccgcag attctgcgcg
      661 gcgtctcgct gatgctcatc atgatcccgg tcaccaccac ggcgcttgga accacgcctc
      721 cgtggaagct caagaacgcc tcgggcctct tcaatctgac gcgcaacctc ggcggcgcgg
      781 ttggactggc gttgatcaat acgcttctga acaagcgcct cgatctgcat ctggcgcgcc
      841 tgcacgagca ggtgacctgg ggacgcgccg ccgcggagga ggcgctcgca aaactgaccg
      901 agcagatggc gcatttcggc tcggacgcct cgctcgccgc cacaaaaaag ctggcactgc
      961 tcgtgcggag gcaggcgacc gtcatggcta tagccgacgt gttctgggcg ctgaccgtcc
     1021 ttttcctgct gatcgcgctc ctggcaccta tcgtgcggcg gcccaaggcg caaggcggcg
     1081 gcggcgggca ctgatactgc ccctgaaatc gaaagcgttc agcccttcac cgagaattac
     1141 gtaaacgcgc ggcggccgcg gagatcaaac tgtcaattgt tacagggaag cgcaaaaagc
     1201 ggctgagcct gaagggtacg cagcctgcgg tagcgccgcg cgctaagctg gcgtggtcag
     1261 ctttttgctt cctgagatac cttaaataaa gctcttcaat ttcatcgtcg tccataatca
     1321 ttgcgtgaga ccgctgatct atgagtctat tgggccaagc tacttgttgg tgagaggacg
     1381 aaccaccaac cagaagttta tccgctcgaa agagtcctat cctgttgcaa ggacactatg
     1441 tagttcacca tgcttgattg ggtcacacac ccgacgccag gctctatcgc aagcgtgact
     1501 gtgcgaggaa aagctttcgc gtaacgagcg gatgtcggcc atgaacggca gctaaaccct
     1561 taacttgagg cgttcccaga aaatcgctcg atgcctgttc acggcgtcaa gcgctataac
     1621 gtcggcgtcg ccgttgccgc tttcggcgct ccacttacgc agaaatgaag agcgccttcg
     1681 tgaaagattt gccgagggca atgacaagaa caatgcctcc cgtgcattct taaaaggagg
     1741 atgggaaggg tcggcaactc ggtgcgagaa atgcctacaa ttatcggcgt cgcgttaagc
     1801 tttacacgcg gaagtggttg ccgatttatt aatatccacc tagatttgat atgaaagggc
     1861 ttggctgaac tgcgcgtaat tgagctaaaa agaggtgagc atgcgagatg ttcaatctta
     1921 tacttcaatc cctacgtggg tgtgggctct gatcacggtc gcgctattat ttctcactat
     1981 tctctacata gagcctgaag cgaggcgctc cttagcgcaa atgttgcttg atagtgagcc
     2041 gagcatttgg cgtcgctttg agcatgaggg ttatcggaaa ggtgggcctt acatcgtcag
     2101 ggctcctctt tactaagaaa aatacctgag acactcccta gaaaagccga atgggcttgt
     2161 cataattccg tttttcgata catcaacccc gtctcttgcg tggccttacc gtcaaactgc
     2221 catttccact ttgcccgcag caaaagattc tgcggccagt agttagaggg gaaaaagcac
     2281 tcggcctgct gcgcgcgtgc atgggttggg aaagcggaaa ccttgcttgg ggcatcatga
     2341 gcgttcttat cgcgtttgaa gaatcaagct ttatatgcgc catgatgaac tgggatcgat
     2401 tggatttccg ccgcctcggt agtgccgcgg ccttggcctt caggctgacc gcggcagcat
     2461 tgctcgctct gtggctcgcc caccgtctca aggttgcctt gccgctctgg tcggtgctga
     2521 cggcgctcat cgtaacgcaa atcagcctgg gacgctccct gaaagcaacc ttggactact
     2581 tcgccgggac catcggcggc gtcgtgtggg gcgcatgcgt tgcgctcctc gtcccgcaca
     2641 ccaccgaagc ctcgctgctc cttgttctgc tgcttgcgct ggcgccgctt gccttcacgg
     2701 cggctctcca tccgtggctg agcgccggcc cgatcacggc ggccatcgtg gtgctgctcc
     2761 cgcaaatgat gcacacatcg ccgatcgatt ccgccataga gcgagtgatc gaggtgttgc
     2821 ttggcggcgt ggcgggcctg ttcgtctcat tcgtgcttgt gccgtggagc gccttccagc
     2881 acacgcgcga agtggcc
//
LOCUS       DATMGX010000918         2824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_347677, whole genome shotgun sequence.
ACCESSION   DATMGX010000918 DATMGX010000000
VERSION     DATMGX010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347677"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VNR65_14995"
     CDS             <1..245
                     /locus_tag="VNR65_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20025.1"
                     /translation="SELSFTPVRRSSKHDAAAAPNLPNIRESASKTATIAPLECASTL
                     AQRARRAKRAGEAAPRLAFGPGVRKAGALKYEGRWG"
     gene            complement(220..2484)
                     /locus_tag="VNR65_15000"
     CDS             complement(220..2484)
                     /locus_tag="VNR65_15000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HWJ20026.1"
                     /translation="MTVENRESVLPANGNQLLQFAPNFTVYALSSDVLCLYSEDRKFL
                     LHGALYVAIGALIAENGKSSQDIVSVLDQYFPSEQIHEALKRLLDRRYVIPKSKSTNG
                     AVAAYWESLGLPVDSAEKNLEQCRIRVHAIDVQGEAELGAALSELGARVVKRSPDLTV
                     ALVSDYLGEPLAELNKRHLSDGTPWILVQPSGVFPLVGPVFTPGKSACWVCLADRMKR
                     NREVRGMLNRSDAKLLTVSPLVQHTVGQSGIQLAAMEITKAIATGFRTDLNNHILSLD
                     LTGSATVKHYVAARPQCRECGSVEFRDSGRAPVPVELKGGGRPVMTSGGYRTMSSHAT
                     VARYRKHISPLTGVVSRLERIETDLPLNTSYFAKHNFSAPSKTVHELRGALEGSSFGK
                     GSTAEQGQASALMEAIERYSGVFQGDEIRVKKRFKDFAQGEAILPNSVLNFSDEQYRQ
                     GLAEMPGPDDEPVSPYLFDTETEMEWSPVWSLRDKCFRYLPTSVLYFYYAGHVIPFHA
                     DSNGCAAGNTREEAIVQGFLELVERDAYAIWWYNRLKRPAVNLDQFNDSYVRDLQIQL
                     AESGKRLWVLDITSDLGIPTFVAIAHWVKDGSEYIDFGSGAHFDARIAMLRTLTELNQ
                     FMSIEIIAGGARMQESLDGVTPLRLADHPFLLPGDGEVLEAEFDSKFSHLETDKQVAA
                     CVAIAERQGLDFLVLDQTRPDIEVPVVRVIVPGLRHFYRRFGPGRLYDVPVKLGWLEQ
                     PLKEEELNPIFPHT"
     gene            complement(2621..>2824)
                     /locus_tag="VNR65_15005"
     CDS             complement(2621..>2824)
                     /locus_tag="VNR65_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20027.1"
                     /translation="GSAVAAVAAVAAAAAEGGVCLGVGADPGAKRKQRSLRSAIVGAG
                     ENCSDPALSLLCAVNAARYGTLN"
BASE COUNT          599 a          879 c          782 g          564 t
ORIGIN      
        1 cgagcgaact cagcttcacg cccgtgcgca gatcgtccaa gcacgacgcc gcagccgcgc
       61 caaaccttcc caacattagg gagtcagcct cgaagacggc aacgattgcg ccgctcgagt
      121 gcgcctcgac cctcgcccag cgggcaaggc gcgcgaagag ggctggcgaa gctgcccctc
      181 gccttgcctt tggtccggga gtgcgcaaag cgggagccct taagtatgag ggaagatggg
      241 gttgagttcc tcttccttga gcggctgttc gagccacccg agcttgaccg gaacatcgta
      301 aagcctgcca ggtccgaaac ggcgatagaa atgccgcaat ccgggcacga taactcttac
      361 aaccggcacc tcgatgtcgg ggcgcgtttg atcgaggacg aggaaatcga ggccctgccg
      421 ctccgctatc gccacgcagg ccgccacttg cttgtccgtt tccaagtgac tgaacttcga
      481 atcgaactct gcttccagca cttcgccgtc gcccggtagg aggaagggat ggtccgccag
      541 ccgtaaaggt gttacgccgt cgaggctctc ttgcattctg gcgccgcccg cgatgatttc
      601 tatcgacatg aactgattca gttccgtcag cgtccgcagc atggcgatgc gcgcatcgaa
      661 atgagcgccc gatccgaaat cgatatattc gctcccgtcc ttcacccaat gcgctatggc
      721 gacgaaggtc ggaatgccaa ggtcgctggt gatgtccagc acccagaggc gcttaccgct
      781 ttccgccagc tgaatttgga gatcccggac gtaggaatcg ttgaactggt caagattcac
      841 ggcggggcgc ttcaaccgat tgtaccacca gattgcgtag gcatcgcgct caaccagttc
      901 gaggaacccc tgcacgatcg cctcttcgcg ggtgttgccg gcagcgcaac cgttcgaatc
      961 cgcatgaaaa ggaatgacgt ggccggcgta gtagaaatac aaaacgctcg tcggaagata
     1021 tctgaagcac ttgtcgcgca aagaccatac aggcgaccat tccatctcag tctcagtatc
     1081 gaaaagatac gggctgactg gttcgtcatc cgggccgggc atttcagcca agccctgacg
     1141 gtattgctcg tcactgaagt tcagaacgct gttcggtaaa atagcctcac cttgagcgaa
     1201 gtccttgaac cgcttcttta ccctgatctc gtcaccctgg aagacgccgg aatagcgctc
     1261 gatagcctcc atgagcgcgc tggcttggcc ctgctcggcg gtgctgccct tgccgaagct
     1321 gctgccttcc agcgcgccgc gcagttcatg aactgttttc gagggcgccg aaaaattgtg
     1381 cttcgcaaaa taggatgtgt tcagcggcag atccgtttcg atccgctcaa ggcgagagac
     1441 cacgccggtg agagggctga tatgcttgcg gtagcgagcc accgtcgcat gcgatgacat
     1501 ggtgcggtag ccgccgctgg tcatgaccgg ccggccgccg ccctttaact cgaccggaac
     1561 tggcgcgcgg cctgaatctc gaaactctac agagccgcat tcccggcatt gagggcgggc
     1621 cgccacataa tgcttgacgg ttgctgaacc agtcagatcg aggctcagaa tgtggttgtt
     1681 caaatccgtg cggaaacccg tagcaatggc cttcgtaatc tccatcgctg cgagctgtat
     1741 gccgctttgg ccaaccgtat gctggaccag gggcgagacc gtgaggagct tagcgtcgct
     1801 tctattcagc atccccctga cttcgcggtt ccgcttcatc cgatcggcga ggcaaaccca
     1861 gcaggcgctc ttgccgggcg tgaaaaccgg ccccaccaag ggaaatacgc cagaaggctg
     1921 aacgaggatc caaggtgttc cgtccgacag atgacgcttg ttcaactccg ccagcggctc
     1981 accaaggtag tcgctgacca gtgccacagt cagatcaggg gagcgcttga cgacgcgtgc
     2041 acccaattcg ctcagcgccg cgcccagttc cgcttctcct tgcacatcga tcgcatgaac
     2101 gcgtatgcgg cactgttcca gattcttttc cgcactgtcc accggaagcc cgaggctttc
     2161 ccaataggca gccaccgcgc cattcgtgga tttcgacttg ggaatgacat agcgacggtc
     2221 aagcaatcgc ttcaaggctt cgtggatttg ctcggacgga aaatattggt cgagcacgct
     2281 cacgatatcc tgagaactct ttccgttctc agcgatcaag gcgccgatcg caacatagag
     2341 cgcgccgtgc agaaggaatt tccggtcctc ggaatagagg caaagcacat cggatgagag
     2401 cgcataaact gtgaaattcg gagcaaactg caggagttga ttgccgttag cgggcaagac
     2461 gctctctcgg ttctcgactg tcataacggg gaaataccct ctcgctggga ctcgtgaagc
     2521 acctatgctc cggccgccag aattccatgt atgttatttg aatcttccgc cagatatcct
     2581 gcgcctgccg ctccacgata cggtttctgc caccctcgcc ctaattgagc gtaccatacc
     2641 gtgccgcatt gactgcgcac aatagactaa gggccggatc gctacaattc tccccggccc
     2701 ccacgatagc gcttcttaag ctgcgctgct tgcgcttagc accagggtct gcacccaccc
     2761 caagacagac accaccctcc gccgccgcag ccgcaaccgc cgcaaccgcc gcaaccgccg
     2821 atcc
//
LOCUS       DATMGX010000919         3221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_443411, whole genome shotgun sequence.
ACCESSION   DATMGX010000919 DATMGX010000000
VERSION     DATMGX010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443411"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VNR65_15010"
     CDS             <1..376
                     /locus_tag="VNR65_15010"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HWJ20028.1"
                     /translation="INLRKGGTTHPFLMLQFLTAGSYDTKTGLFLTAAGKSCSYYSSD
                     NLHEDHYRGLTPSDLIDIAATKGLHFNAATQEGVVFHLIGALSEFGKLGVLAIGDTDE
                     KAYASYMRSVHILDQESTSNGD"
     gene            complement(393..1478)
                     /locus_tag="VNR65_15015"
     CDS             complement(393..1478)
                     /locus_tag="VNR65_15015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWJ20029.1"
                     /translation="MDVSIGTRVRIVLAGVAALFACLQQAAAGPALLTEPSTGLVLYA
                     EDADQPWRPASLTKLMTAYLTFEAIRDGQLSPEDVVTSTTLSQSQPPSKLGMPLGAQL
                     TVDLAIKALIVKSANDVAIMLAEKGGGSIESFVDMMNKTAMRLGMTNTHFVNPNGLPV
                     FELDGNTEAPEQAVTTARDMALLANMILREFPQYSGIFALTEVRIGRRLVTTHNGLLK
                     SYDGADGMKTGFVCAAGYNVVASATRNGRQLVAVVLGERSGAARTIRAAGLFEYGFEM
                     YPWKAVLAPTLATWPVETPQGAKAPDLRNIVCNGRKLAKLANKEKKGKRPALGPGHRM
                     PAQDKPAKGNKKKPIQADAGGSALRPR"
     gene            complement(1499..1584)
                     /locus_tag="VNR65_15020"
     tRNA            complement(1499..1584)
                     /locus_tag="VNR65_15020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1549..1551),aa:Leu,seq:caa)
     gene            complement(1788..3140)
                     /locus_tag="VNR65_15025"
     CDS             complement(1788..3140)
                     /locus_tag="VNR65_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWJ20030.1"
                     /translation="MAKSRSTPSAPLFESVAAIDNASRPLADRLRPASLGEVIGQDHL
                     LGEGGPLRRIAEGERPRSMIFWGPPGTGKTTVARLMARGAGMHFEQLSAIFSGVQDLR
                     KVFEEARDRQRFGQGTILFVDEIHRFNRSQQDSFLPYVEDGTIILVGATTENPSFELN
                     GALLSRAKVLVFKRLTDDDLEHLLRRAETVLGKTLPLTPDARQTLIGMADGDGRSVLN
                     LAEDVLATAHDIAKSIEPDELVSIVQRRAPLYDKNRDGHYDLISALHKAVRGSDPDAA
                     LYWFCRMIDGGEAPLYLARRMVRMAVEDIGLADPQALVQANAAKDAYDFLGSPEGELA
                     LAQCVIYLACAPKSNAGYTAFGAAMRYAKDGGSLPPPKHILNAPTKLMREEGYGAGYV
                     YDHSTEEGVSGQNYFPEGQERQQFYRPKGRGYEREIEARLKSWADVRRSAGKRGGKQD
                     "
BASE COUNT          628 a         1001 c          925 g          667 t
ORIGIN      
        1 aatcaacctg cggaaaggcg gtacaacgca tcctttcctc atgctgcagt ttctgacagc
       61 aggaagctac gacacgaaga caggcctttt tctaaccgcc gccggcaaat cctgctccta
      121 ctactcatcc gacaatcttc acgaggatca ctatcgcggg ctgacgccat cggaccttat
      181 cgatatcgcc gcgacgaaag gcctgcattt caacgccgcg acccaggaag gcgtcgtctt
      241 ccacttgatc ggcgcgctct cggagtttgg caagctcggc gtgctcgcga tcggagacac
      301 ggacgaaaaa gcctacgcct cttacatgcg aagcgtgcac attctcgacc aggaaagcac
      361 gagcaacggc gactaagccc cgcagggccg cgttatcgtg gtctaagcgc agagccgcca
      421 gcgtcagcct ggataggctt ctttttattg cccttcgccg gcttgtcctg agccggcatg
      481 cggtgaccag ggcctaaggc cgggcgcttg cccttcttct ctttgtttgc gagtttcgca
      541 agtttgcggc cattgcaaac gatgttgcgc aaatcgggag ctttcgcgcc ctgcggcgtc
      601 tccaccggcc atgtcgccag cgtgggcgcc aacaccgctt tccaggggta catctcgaaa
      661 ccgtactcga aaagccctgc tgcgcggatc gtgcgcgcgg ctccggaccg ttcccccagc
      721 accacagcga caagctgccg tccattccgc gtcgcgctgg caacaacatt ataaccagcc
      781 gcgcagacga agcccgtctt catgccatcc gcaccatcgt agctcttcag aagtccattg
      841 tgcgtggtca cgaggcggcg cccaatccgc acctccgtga gtgcgaagat gcccgaatat
      901 tgcgggaact ctctcagaat catgttcgcc aaaagagcca tatcgcgcgc cgtcgtgacc
      961 gcctgttccg gcgcttcggt gttgccgtcc agctcgaaca ccggcaggcc gttcggattg
     1021 acgaaatgcg tgttcgtcat gccaaggcgc atggccgtct tattcatcat gtccacgaag
     1081 ctttcgatcg acccgccgcc tttttcagcc agcatgatcg cgacatcgtt cgcggatttc
     1141 acgatcagcg ccttgatagc aagatcgact gtgagctgcg cgccaagcgg catgccgagc
     1201 ttgctcggag gctggctctg cgagagcgtt gtggaggtga caacatcttc aggcgaaagc
     1261 tgaccgtcgc gaatcgcctc gaaagtaagg taggccgtca tcagctttgt gagagacgct
     1321 ggccgccacg gctgatcggc gtcctccgca taaaggacaa gccccgtgga tggttcggtg
     1381 aggagcgcag gcccggcggc ggcctgctgc aagcaggcga ataaagccgc gacccctgcc
     1441 aatactatcc gaactctcgt tcctatcgat acgtccatct ataaaagaca ctcgattttg
     1501 gtgccccagg ccggacttga accagcacgc ccttgcaggc aacagatttt gagtctgccg
     1561 cgtctaccat tccgccactg gggcactccc atcgggatgc gggatcataa cgccgaacta
     1621 cggtgcgtca actcctgtca gtggtcagaa cacacaatag gcttgcacga gactggaagc
     1681 tttggctttt gggcttgatt tctaactcag ttcgtaactt ttgcaccgat cgtggtttaa
     1741 aaactgttga ttagtttgca taaacccagc ctgggcgggt gggccgtcta atcctgcttt
     1801 ccgccgcgtt tgcccgcgct tcgccggaca tcggcccagc ttttcagtcg agcctcgatt
     1861 tcccgttcat atccgcgtcc tttcggccgg taaaattgct gccgctcctg cccttcgggg
     1921 aaatagttct ggccggacac gccttcttcg gtgctgtggt cgtagacgta gcctgcgccg
     1981 tagccttcct ctctcatgag cttggtcggc gcgttcagaa tgtgtttcgg cggcgggagc
     2041 gatccgccgt ccttcgcgta gcgcatggcc gccccgaacg ccgtatagcc cgcattcgat
     2101 tttggcgcgc aagcgagata gatgacgcat tgcgcgagcg cgagttcgcc ttcagggctt
     2161 cccaggaaat cgtaagcatc cttcgcggcg ttcgcctgca cgagcgcctg cggatcggcg
     2221 aggccgatgt cctcgaccgc catgcgtacc atgcggcgcg cgaggtacag cggggcttcg
     2281 ccgccatcga tcatgcgaca gaaccaataa agcgcggcgt cgggatcgga gccgcgcacc
     2341 gccttgtgaa gagcgctgat aaggtcgtaa tgcccgtcgc ggttcttatc gtagaggggc
     2401 gcgcggcgct gcacgatgga gacgagttcg tccggttcga tggacttcgc gatgtcgtgc
     2461 gcagttgcaa ggacatcctc agccagattc agcaccgagc ggccgtcgcc atccgccatg
     2521 ccgatcaggg tttgtcgcgc gtcgggcgta agcggtagcg tcttccccag caccgtctct
     2581 gcccgcctca agaggtgctc aaggtcgtca tcggtgagac gcttgaagac cagcaccttc
     2641 gcgcgggaca gaagcgcgcc gttgagttcg aaagacggat tttcggtcgt ggcgccgacc
     2701 aatatgatcg tgccgtcctc gacataggga agaaaggaat cctgctggga acggttgaac
     2761 cggtggattt cgtcgacgaa aaggatagtg ccctggccga aacgctgcct gtcgcgcgcc
     2821 tcttcgaaaa ccttccgcaa atcctggacg cctgaaaaga tcgccgaaag ctgctcgaaa
     2881 tgcatgccag cgccgcgcgc catgagccga gcgaccgtcg ttttgccggt tcctggcgga
     2941 ccccagaaga tcatcgaccg cggtctctcg ccttcagcaa tgcgtcgcag cggaccaccc
     3001 tcgcccaaca ggtgatcctg cccaatcact tcgccaagcg aagcaggacg cagcctgtcc
     3061 gccagcggac gggatgcgtt atcgatcgcg gcaacgctct cgaaaagcgg cgctgacgga
     3121 gtggatcggg atttagccat gggagacgcc ccgttatgaa ggggcaactt tacacgattt
     3181 caggtagcgt ccagtttgaa ttttttcggc accgatttcc t
//
LOCUS       DATMGX010000920         2909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_207546, whole genome shotgun sequence.
ACCESSION   DATMGX010000920 DATMGX010000000
VERSION     DATMGX010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207546"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VNR65_15030"
     CDS             <1..1190
                     /locus_tag="VNR65_15030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWJ20031.1"
                     /translation="IDYKAVAAHVQGVIDAIAPNDSVERFNGLGVDVIKETARFIDKT
                     TVEAGDYQIKARRFIIATGSAPQIPPIPGLDRIAYFTNETIFANKHRIGHLIIIGGGA
                     VGLELAQAHRRLGSEVTVVEAGRALSRDDPELKEYLLKRLREEGVRVLENARVERIEP
                     FGSNLQVIFATLGKSFSIEGTDLLLATGRAPTVAGLNLEAASVTYSDRGIKVTKGLVT
                     SNKKIYAIGDVTGEAHLTHAANHHATVVIKNALFRLPAKADHETMPWVTFTDPEVAHV
                     GLTEEKARELYGKLAIMRWPYNENDRAQAERLTGGFLKVIASPRGKILGAGIVGAQAG
                     EIIQMWSLAMQKGISLSDMRSIVSPYPTLAEINKRAAVSYFEPRAQHPLVRRIITLLA
                     KFG"
     gene            1325..2773
                     /locus_tag="VNR65_15035"
     CDS             1325..2773
                     /locus_tag="VNR65_15035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWJ20032.1"
                     /translation="MWSIATSLPAKLLFLTIAFVMLAEVLIFVPSIANFRRNWLMERV
                     MAAKIASLALEASGGAEVPERLRQELLTTAGVHAVSVRRPESRRLVLGMPDEAQIVDV
                     YDLRTENFVQLIPDALQALAAPPGRLIRVIAAPDMMTSGDEIDVAIDETPLRAAMWRY
                     ARNIFWLSIVISIFTAALVYLALTGLLVRPMMRITRNIVTYRENPEDTSRIITPSGRH
                     DEVGVAEKELATLQMQLSGLLREKARLANVGLAVSKINHDLRNLLSSAQLVSDRLATV
                     ADPTVQRFVPRLIRALDRAIVLCTNTIKYGRSEEAPPKRTRVPLRALLTEVNESLGVE
                     EFSGVRMTISVPQTINLNADRDQIFRVLANLVRNALEALRQADPPVNDPEIEVTARQE
                     GETAIVKVRDNGPGVPAKVRETLFKAFQSSSKSDGNGLGLVIAAELVRAHGGDIRLED
                     TASGASFIVKLPASVLAGTPSKPPFAPPILTQ"
BASE COUNT          591 a          877 c          882 g          559 t
ORIGIN      
        1 gcatcgacta caaggcggtt gccgcgcatg tgcaaggggt catcgacgcc attgcgccga
       61 acgactccgt cgagcgtttc aacgggctcg gcgttgatgt cataaaggag acagcccgat
      121 tcatcgacaa gactacggtc gaggcgggtg attatcagat caaggcgcgc cgcttcatta
      181 tcgcgacggg gtccgcgccc cagataccgc ccattccagg gctcgaccgc attgcgtatt
      241 tcacgaacga gacgattttc gccaacaagc atcgcatcgg gcatctcatc atcattggcg
      301 gcggcgcggt aggtctggaa cttgcccaag cccaccggag gctcggcagc gaggtgaccg
      361 tcgttgaagc gggccgcgct ttgtcgaggg atgatccgga actcaaggaa taccttctga
      421 aacgcctgcg cgaggagggc gtacgcgtgc tcgaaaacgc gcgcgtggag aggatcgagc
      481 ccttcggctc gaacttgcag gtcatcttcg caacgctggg aaaatccttc tcgatagaag
      541 ggaccgacct gcttcttgca acaggacgcg cgccgacggt cgctggcctc aatctcgaag
      601 ccgccagcgt aacctatagc gaccgcggca ttaaagtgac caagggcctc gtaacatcca
      661 acaaaaagat ctacgccatc ggcgacgtta ccggcgaagc ccatctgacg catgctgcga
      721 accatcatgc aaccgtcgtc atcaagaacg cgctgttccg gctgccggca aaagctgacc
      781 atgagacgat gccctgggtg acgttcacgg atccggaagt ggctcatgtc ggcctgacgg
      841 aagaaaaggc gcgggagctc tatggaaagc tcgcgatcat gcgctggcct tataacgaga
      901 acgatcgcgc tcaagccgag cggctgacgg gagggttcct gaaggtcatc gcttcgccac
      961 gcggcaagat ccttggagcg ggcatcgtcg gcgcgcaggc aggcgagatc attcagatgt
     1021 ggagccttgc catgcaaaag gggatatccc tcagcgacat gcgcagcatc gtatcgcctt
     1081 atccaacgct tgcggaaatc aataagaggg ccgcagtgag ctatttcgag ccccgtgcgc
     1141 aacatccttt agtgcggcga attattacgt tgcttgcgaa attcggctaa tggtcgttat
     1201 ctaggaggag agtgcaacaa cgggctcttt gtcgcaattg gatacccgac cctcacataa
     1261 cccggagtat ccgggccagt tgctcccgga gcaaccccgc ccaccccggc agccatacgg
     1321 gggtgtgtgg agcattgcca cgagtctgcc cgccaagctt ctctttctca ccattgcctt
     1381 tgtgatgctg gccgaggtgc tcatcttcgt accgtccatc gcgaacttcc gccggaactg
     1441 gctgatggag cgcgtcatgg cggcgaaaat cgcatctctg gctctggagg cctcgggcgg
     1501 cgcggaagtt ccggagcgct tgcgacagga gctgttgacg acagccgggg ttcacgccgt
     1561 ctctgtgagg cggccggaat cgcggcggct tgtgctcggc atgcctgatg aagcccagat
     1621 cgtggatgtc tatgatctgc gcacggagaa tttcgtgcaa ctcataccgg atgctctcca
     1681 agcgctcgca gctcctccgg gccggctcat tcgcgttatc gcagcgcccg acatgatgac
     1741 gtccggtgac gagatcgatg ttgcgatcga tgagacgcca cttcgcgccg ccatgtggcg
     1801 ttacgcccgg aacatcttct ggctctcgat cgtcatttcc atcttcaccg cggcgttggt
     1861 ctatttggcg ctgacggggc ttctcgtgcg gcccatgatg cgcattacgc gcaatattgt
     1921 gacgtatcgc gaaaacccgg aagacacgtc caggatcatc acgccatcgg ggcggcacga
     1981 tgaggttggc gtcgccgaaa aggaactggc gacgctgcaa atgcagcttt ccggtcttct
     2041 aagggagaag gcgcggcttg cgaatgtcgg gctcgccgtc tcgaagatca accatgatct
     2101 gcgaaatctt ctgtccagcg cgcagcttgt ctccgacagg cttgccacgg ttgctgaccc
     2161 gacagttcag cgcttcgtgc cgcgcctcat tcgtgcgctc gaccgcgcta tcgtcctctg
     2221 cacgaacacg atcaagtatg gccgcagtga agaggctcca ccgaagcgca cgcgcgtgcc
     2281 ccttcgggcg ctgctcaccg aggtgaacga aagcctcggg gtggaggaat tcagcggcgt
     2341 tcgcatgaca atctccgtgc cgcaaacgat caatcttaac gccgaccgcg atcagatctt
     2401 ccgggtcctc gcgaacctcg tacggaatgc attggaagcg ctgcgccagg ccgatcctcc
     2461 cgtcaatgat ccggagatcg aagttacggc gcgtcaggaa ggcgagactg caatcgtcaa
     2521 ggtgcgtgac aacggcccgg gagtacccgc caaagtcaga gagactctgt tcaaggcgtt
     2581 ccagagctca tccaagagcg acggaaacgg cctcggcctc gtgattgccg ctgaactggt
     2641 ccgcgcccat ggcggcgata tccgtcttga ggacacggcc tccggggcgt ctttcatcgt
     2701 gaagctcccg gcgtctgttc tggccggcac gccgtcgaag ccgccgtttg cgccgccaat
     2761 attgacgcaa tagcgcggaa ggctgcgtgg cgaggcccgt gaagggtccc atgcgcgcga
     2821 gacttgcgaa gccagatcgc gcaggcctct aaagtgccgg agcagtccat ccgcatccaa
     2881 ctggacgttg acctgcctag cgggcggcg
//
LOCUS       DATMGX010000921         3450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_79462, whole genome shotgun sequence.
ACCESSION   DATMGX010000921 DATMGX010000000
VERSION     DATMGX010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79462"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..85
                     /gene="rpsK"
                     /locus_tag="VNR65_15040"
     CDS             <1..85
                     /gene="rpsK"
                     /locus_tag="VNR65_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWJ20033.1"
                     /translation="CGFQVTSIRDVTPIPHNGCRPRKRRRV"
     gene            156..1178
                     /locus_tag="VNR65_15045"
     CDS             156..1178
                     /locus_tag="VNR65_15045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWJ20034.1"
                     /translation="MKVLNKNWQDLIKPGKINVIPGANPKYLASIVAEPLERGFGLTL
                     GNALRRVLLSSLQGGAVTTVHIDGVLHEFSSIPGVREDVTDIVLNIKDIALRIHSEGV
                     KRMVLRKEGPGVARARDIDAGPDVEILNPDLVICTLDEGAAVRMELTANIGKGYVAAE
                     RNRPDDAPIGLVPVDSLYSPVKKVSYKIANTREGQILDYDKLTLEVETNGAVSPEDAV
                     ALAARIIQDQLSIFVNFEEPRKEVSEERIPQLEFNAALLKKVDELELSVRSANCLKND
                     NIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLAGMGLHLGMEVPNWPPENIEELAK
                     RFEDHY"
     gene            1231..1656
                     /gene="rplQ"
                     /locus_tag="VNR65_15050"
     CDS             1231..1656
                     /gene="rplQ"
                     /locus_tag="VNR65_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWJ20035.1"
                     /translation="MRHRTSGRKFNRTASHRKAMFANMAAALVKHEQIVTTLPKAKDL
                     RGVVEALITLAKRGDLHARRVVAARLRDETTVSKLFDTLGPRYRSRSGGYTRVLKAGF
                     RYGDNAPLAVIELVDRDENAKGLDSGPVAGEEDEQSAAA"
     gene            2020..>3450
                     /gene="uvrB"
                     /locus_tag="VNR65_15055"
     CDS             2020..>3450
                     /gene="uvrB"
                     /locus_tag="VNR65_15055"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWJ20036.1"
                     /translation="MPLFVPHRPSRPEKSEGGKPFVIKSDFDPKGDQPRAILELVEGV
                     GQLEREQVLLGVTGSGKTFTMAKVIEETQRPALILAPNKTLAAQLYGEFRSFFPDNAV
                     EYFVSYYDYYQPEAYIPRSDTYIEKDSSINEQIDRMRHAATRALLERDDVIIVASVSC
                     IYGIGSVETYSAMTFTIQVGDRVAQRQLLADLVAIQYKRNNVSFARGDFRVRGDTVEI
                     FPAHQEDRAWRICMFGDEVESIAEFDPLTGEKTQDLALVKVYANSHYVTPKPTLHQAI
                     KGIQQELQEQLQRFNATGRLLEAQRLEQRTQFDIEMIEATGSCNGIENYSRWLTGRLP
                     GEPPPTLFEYLPDNALVFVDESHVTVPQIGGMFRGDFRRKSTLAEFGFRLPSCIDNRP
                     LRFEEWDAMRPQTTFVSATPGKWEIEQTGGVFVEQVIRPTGLIDPPVEIRPVKTQVDD
                     LIDECRKVIKQGYRVLVTTLTKRMSED"
BASE COUNT          770 a          956 c         1035 g          689 t
ORIGIN      
        1 ttgcggtttt caggtcactt caatccgcga cgttacacct attccgcata acggctgccg
       61 cccgcggaag cggcgccggg tctaagcggt ctttgggcaa gccacccgtg ttagcaagat
      121 cgcggcgaag agcgtgagcg cggccttata aggaaataaa agtgcttaat aagaattggc
      181 aggatctcat caagccggga aagatcaacg ttatccccgg tgctaacccc aaatatctgg
      241 cgtcaattgt ggctgagccg ctggagcgcg gatttgggtt gacgcttggt aatgcgctcc
      301 gccgcgtcct gctgtcctct ttgcagggtg gagccgtaac gactgttcat atcgacggcg
      361 tgcttcatga gttctcgtcc ataccgggcg tgcgcgagga tgtgacggat atcgtcctca
      421 atatcaagga tatcgcgctt cgaattcact cggaaggcgt caaacgcatg gttcttcgca
      481 aagaaggacc gggcgtcgca cgggcgcgtg acatagacgc tggaccagat gttgaaatcc
      541 ttaatcctga tctggtgatc tgcacactgg acgagggtgc ggccgtccgc atggagttga
      601 ccgcgaatat cggtaagggc tacgtggcgg ctgagcggaa tcgcccagac gatgctccga
      661 ttggacttgt cccggtggac agcctttaca gtccggtgaa aaaagtttct tacaagatcg
      721 ccaatacacg cgaggggcag atcctcgact acgataagct gacgcttgag gtcgagacca
      781 acggggccgt gtcacctgag gacgccgttg ctcttgcagc gcggatcatt caggatcagc
      841 tgtcgatatt cgtgaacttc gaagagccgc gaaaggaagt ctcggaagag cgcattccgc
      901 agcttgagtt caacgccgcg ctcctgaaaa aggttgacga acttgagctt tccgtccgct
      961 cggcaaattg cttgaagaac gacaacattg tctatatcgg cgatctcatt cagaagaccg
     1021 aagcggaaat gttgcgtaca ccgaatttcg ggcgtaagtc gttgaacgag attaaagagg
     1081 tgttggccgg tatggggctg catctcggca tggaagtccc gaattggccg ccagagaaca
     1141 tcgaagagct ggcgaagcgg ttcgaggacc attactgatt agggatagac tatcgcggcg
     1201 ttggacgctg cgggctttga gggcgagaca atgcgtcacc gtacatccgg caggaaattc
     1261 aaccgaacgg caagccatcg taaggctatg ttcgccaaca tggcggctgc gctcgtaaag
     1321 catgagcaga tcgtaactac gcttcccaag gcgaaagacc tgcgcggtgt ggtggaggcg
     1381 ctcatcacac tcgccaagag gggtgacctt catgcacggc gggtcgtggc ggcgcgcctc
     1441 cgcgacgaga ccacggtgtc taagctcttt gatacgctag ggccacgata tcgaagccgc
     1501 tccggagggt atacccgcgt tctcaaggcc ggcttccgct atggcgataa cgcgccgctt
     1561 gctgtcatcg agcttgtcga tcgcgatgaa aacgccaaag gcttggattc aggcccggtt
     1621 gcaggcgaag aggacgaaca aagcgcagcg gcgtaacgat ccagacgcag aattaaggcc
     1681 ggcttgactg tgttaagcgg gccttttttg tgcctaaagc aagacacggt agcgaaatct
     1741 cgattcgaat gacatatatg gcgcatggca cgagcgcgcg gggctgagtc atctgcccgg
     1801 aacaagcaga ccttcaaaca agcgggagat ggcgacacga ttgggttcgc tgaagcgccg
     1861 caagctgcgt tcgagggcca agcctacacc tcggaaagta cgtcgcatct catccccgca
     1921 cttcgaaatt tgaagccgac gggcgcgatg ccgctgccat cttatctcca gggcaagctg
     1981 cctcctatcg gcgaccaccc gcttctcacc ggcaacgata tgccgctttt cgtgccgcat
     2041 cgtccatcgc ggccggagaa gagcgaaggt ggaaagcctt ttgttatcaa atcggatttc
     2101 gacccgaaag gcgatcagcc gcgggctatc ctcgaactgg tggagggggt gggccagctt
     2161 gagcgggagc aggttctgct tggtgtcacg ggttccggca agaccttcac catggctaag
     2221 gttatcgagg aaacacagcg acctgcgctg atcctggcgc cgaacaaaac gctagcagcg
     2281 cagctatacg gtgagttcag gtcgttcttc ccagacaacg ccgtcgagta tttcgtctcc
     2341 tattacgact actaccagcc ggaagcctat attccgcgga gcgacaccta tatcgaaaag
     2401 gatagctcga tcaacgagca gatcgaccgg atgcgccacg ccgccacgcg tgcgctgctc
     2461 gagcgtgacg atgttattat cgtggcgtcg gtctcttgca tttacggcat cggctcggtc
     2521 gagacctatt ccgcgatgac gttcaccatc caggtgggtg accgggtggc gcaacggcaa
     2581 ctcctggccg atctcgttgc catccagtac aagcgcaaca atgtgagctt cgcccgcggt
     2641 gatttccgtg ttcgcggaga caccgtcgaa atcttcccgg ctcaccagga ggatcgggcc
     2701 tggcgtatct gcatgttcgg cgacgaggtg gaaagcatcg ccgagttcga tccgctgacg
     2761 ggagagaaaa cgcaggacct ggcactcgta aaggtctacg cgaattcaca ctatgtgacg
     2821 ccgaagccga cgcttcatca ggcgatcaag ggtattcagc aggagttgca ggagcagttg
     2881 cagcgtttca acgccactgg gcggcttctc gaagcacagc ggctggagca acgtacgcaa
     2941 ttcgacattg agatgatcga ggcgaccggc tcatgcaacg gcatcgagaa ctattcgcgc
     3001 tggctcactg gccgcctgcc cggcgagccg ccgccgacat tattcgaata tctgccggac
     3061 aacgcgttgg tcttcgtaga cgaaagccac gtcaccgttc cgcaaatcgg cggaatgttc
     3121 cgcggcgact tccgccgcaa atcgacgctc gccgagttcg gcttccgcct gccatcctgt
     3181 atcgacaatc ggccgcttcg cttcgaagaa tgggacgcga tgcggccgca gaccacgttt
     3241 gtatcggcca cgcctggcaa gtgggagata gagcagactg gcggcgtgtt cgtggaacag
     3301 gtcatccgcc cgacaggcct tatagatccg cccgtggaaa ttcgcccggt caaaacgcag
     3361 gtggacgacc tgattgacga atgccgcaag gtcatcaagc agggctatcg cgtgctggtc
     3421 acgacgctca ccaagcgcat gtcggaggac
//
LOCUS       DATMGX010000922         5184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_86302, whole genome shotgun sequence.
ACCESSION   DATMGX010000922 DATMGX010000000
VERSION     DATMGX010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86302"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VNR65_15060"
     CDS             <1..280
                     /locus_tag="VNR65_15060"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HWJ20037.1"
                     /translation="DDHCSACGQDFTPHRADDLPAYLVIVIVGHIMVPTILLIETDYS
                     LSTALQLSIYLPLVLVLSLVLLQPVKGAVVGVQWALRMHGFDEQNPTE"
     gene            329..1069
                     /locus_tag="VNR65_15065"
     CDS             329..1069
                     /locus_tag="VNR65_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20038.1"
                     /translation="MTETENREKTEKEADHHPYRRPVDAATLILVDRTVSTPKVLVGK
                     RHDKVVFMPGKFVFPGGRVDKTDNRVPVAAPIPKALEENLLKGSPKITASRARSLTIA
                     AIREACEETGLCLGRKGNGAVPDLEGPWKSFTEAGLLPDPSGLFLIARAITPPGRVRR
                     FDTRFFTADASAIAHRVEGVIHADAELVELVWVELGSKPLADLHPMTRNVLNELEKRL
                     ATGPLGHDAPVPFFHFYGGKMQKDVLGG"
     gene            complement(1171..2388)
                     /locus_tag="VNR65_15070"
     CDS             complement(1171..2388)
                     /locus_tag="VNR65_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HWJ20039.1"
                     /translation="MAVDLFDNWFDPIETEVRARARQFIEELIRGELDTALARPRYER
                     SKMAGNEAGACVKGHRHGSRTRSLTGTFGPIEIAVPRARLTTPEGGTAEWRSQALRAY
                     QRRTLAADALIASSYLAGTNTRRVRRSLAALFGGTVGKDTVSRTWRKVKGDWDAWNSR
                     SLAAEPIVRLILDGTVVRVRLDRKATSISLLVVLGVRADGQKVLLAIKSMGGESAEAW
                     RTVLDDLIKRGLRRPEFLIVDGAPGLDKAIAVVWDGVPVQRCTVHKHRNLLAHAPERL
                     HDEITADYNDMIYATTPEEITVRRKAFIRKWRLKHRAVADSLEEAGDRLFTFARLPQG
                     QWRSLRTTNAIERLHEEFKRRIKTQTVLPSADTAAMMFWALLASGQISMRKVDGWNTL
                     ATKPTDQPIDLAA"
     gene            2420..3001
                     /locus_tag="VNR65_15075"
     CDS             2420..3001
                     /locus_tag="VNR65_15075"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HWJ20040.1"
                     /translation="MVSLIMAWSPVRRFNAGRFEVHHPGDYANLNSYQLRDGTREHPE
                     ITTQIQTSVADVTGELHSIFGPASKNYFCAKKAVSELHRYGNLHEKTVFEYAQSHKLQ
                     EVTIALSLLCSLPANVIERGLIDSTGEMPMIFAKALEFSWETMMSLLFLGAPNYKISA
                     RDLDALKVKFSHLTVETAKNLIRLYQSRKATSD"
     gene            3246..4220
                     /locus_tag="VNR65_15080"
     CDS             3246..4220
                     /locus_tag="VNR65_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20041.1"
                     /translation="MKVAAVKFLWAVAAGLVASAPIATITLSSVGAAEECLTTPKNET
                     PPGKHWYYRSEGGTKRLCWYLREEGETPSQTATSTPGRRTALEAAPNRETKLARSAAD
                     AHAELPLPQTRVEADPKISPTTPATSFNPRGAAQKLSSNASPETARPPVASRRPEPTG
                     VFSSPTEGPISPSFVVASPAPDTNSDASADTDLAPKVPPVVRTKVETSAMGTPASLRI
                     LLLGTFGAIAVLWLTGSAVMARMRRRPQRYTRSNPHTDLNPPERSTEEPTNHTGIPPS
                     RKPMPVNSPRRRNAGSQSSDRQRSGLGDNAREIEQLLARFTKQAQTEP"
     gene            complement(4395..4583)
                     /locus_tag="VNR65_15085"
     CDS             complement(4395..4583)
                     /locus_tag="VNR65_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20042.1"
                     /translation="MTDQWNEALRCPQCSNTGIASLSQFEAANMPTVDNVPDGFKVVQ
                     TEFGPDFYCGTCNVPALP"
BASE COUNT         1061 a         1558 c         1538 g         1027 t
ORIGIN      
        1 cgacgatcac tgctcggcgt gcggacagga cttcacgccg caccgcgccg atgacctgcc
       61 ggcctatctg gtcatcgtca tcgtcggcca tatcatggtg ccgacgatcc tgttgatcga
      121 aaccgattat tcgctttcga ctgcgctgca actctcgatc tatctgccgt tggtgctggt
      181 cctgtcgctg gtgctgctgc aaccggtcaa gggcgccgtg gtcggggtgc aatgggcgtt
      241 gcgcatgcat ggattcgacg agcaaaatcc gacagaatga tcggatgatt tcaacaacaa
      301 gaaacgagac tggggcagtg gaagcgaaat gaccgagacc gagaacaggg aaaagaccga
      361 gaaggaagcc gaccatcacc cctaccgccg cccggtcgac gccgccacgc tgatcctggt
      421 cgaccgcacc gtctcgacgc cgaaagttct ggtcggcaaa cgccacgaca aggtggtgtt
      481 catgcccggc aagttcgtct ttcccggcgg ccgcgtcgac aagaccgaca accgcgttcc
      541 cgtggctgcc cccatcccga aggcgctgga ggaaaacctc ctgaagggca gcccgaaaat
      601 taccgcatcc cgcgcgcgct cgctgacgat tgccgccatc cgcgaggcct gcgaggaaac
      661 cggcctttgt ctgggacgca agggcaatgg tgctgtgcct gatctggaag gcccatggaa
      721 gtcgtttacc gaggcaggcc tgctgcccga cccatccggc ctgtttctga tcgcgcgcgc
      781 gatcacgcct cctggacgtg ttcggcgctt cgatacccgc ttcttcaccg cggacgcctc
      841 cgccatcgcc catcgcgtcg agggcgtgat ccatgccgat gccgaactgg tcgaactggt
      901 ctgggtcgag ctcggttcga aacccttggc cgacctccat cccatgacca ggaatgtttt
      961 gaacgaactc gaaaagcgtc tcgccacggg gccgctgggc cacgacgcgc cggtaccgtt
     1021 cttccatttt tacggcggca agatgcagaa ggacgtgttg ggggggtaac gctccctccc
     1081 gttattccgg ggcgcgcgaa gcgcgggtgc cgtccggaat gcggttggaa ttcggtatgg
     1141 cgtattcccg ggtaacatga ggttatcgtt tcaggcggca aggtcaattg gctgatcggt
     1201 gggttttgtg gcgagcgtgt tccagccatc caccttgcgc atgctgatct gtcccgaggc
     1261 gagcagcgcc cagaacatca tcgcggcggt gtcggccgac ggcagtacgg tttgggtttt
     1321 gatccggcgc ttgaactcct cgtgcaatcg ctcgatcgca ttggtggtgc gtaggctgcg
     1381 ccattggcct tgcggcaggc gcgcgaaggt gaacaagcgg tctcccgctt cctccaggct
     1441 gtcggcaacg gcacgatgct tgagccgcca tttgcggatg aaggccttgc ggcgaacggt
     1501 gatctcctca ggcgtcgtcg catagatcat gtcgttgtaa tccgcggtga tctcgtcgtg
     1561 caggcgctcc ggggcatgcg ccagcaggtt tctgtgcttg tgaaccgtgc agcgttgcac
     1621 cggcactccg tcccagacga ccgcaatggc cttgtcgagc cctggtgcgc cgtcgacgat
     1681 gaggaattcg ggacgccgca gcccgcgctt gatgagatcg tccagcaccg tgcgccatgc
     1741 ctcggcgctt tcgccgccca tgctcttgat cgcgagcaga accttttggc cgtccgctcg
     1801 cacgcccagc acaacgagca gtgaaatcga ggtcgccttg cggtcgaggc gcacccgaac
     1861 aacggtgccg tcgaggatca ggcgcacgat cggctccgcg gccagcgagc ggctgttcca
     1921 ggcgtcccag tcgcccttca ccttgcgcca ggtccggctc accgtgtcct tgccgaccgt
     1981 cccgccgaac aaggcggcca gcgagcgacg cacccgcctg gtattggtgc cggccaggta
     2041 gctgcttgcg atcagcgcgt cggcggcaag cgtgcgtcgc tgataggccc gcagtgcctg
     2101 gctcctccat tccgccgtcc cgccctccgg cgtggtcagc cgggcccgcg gcaccgcgat
     2161 ctcgatcggc ccaaacgtgc ccgtcagcga gcgcgtccgg ctgccatgac ggtggccctt
     2221 gacgcaggct cctgcttcat tgccggccat cttgcttcgt tcgtaacgcg gccgtgccag
     2281 cgcggtatcg agttcgccgc ggatcagctc ttcgatgaac tgacgcgccc gagcccgtac
     2341 ctcggtctcg atcgggtcga accagttgtc gaaaagatca acagccatat cagtcgcagg
     2401 ctgcgtggaa ttaggcttcg tggtatcctt gatcatggcg tggtctccag tccgacgctt
     2461 caacgccgga cgattcgagg ttcatcaccc cggagactac gccaacctca attcctacca
     2521 actccgcgac ggcacccgtg agcatcctga gataacgact caaattcaaa cgtcggttgc
     2581 ggatgtcact ggcgaactgc attcgatatt cgggcctgca tccaagaact atttttgcgc
     2641 aaaaaaggcc gtttccgagc ttcatcgata cggtaatctg catgagaaaa ctgtttttga
     2701 atatgctcag tcccataaat tgcaggaagt aacgatagcg ctctccttgc tatgctctct
     2761 gcctgcaaac gtcatagagc gagggttgat tgacagtacg ggcgaaatgc caatgatttt
     2821 tgccaaagcg cttgaatttt cgtgggagac catgatgtcg cttctctttc tcggggctcc
     2881 aaactataaa atctctgcgc gcgaccttga tgcgttaaag gtgaagtttt ctcacctcac
     2941 ggtcgagacg gctaaaaact taattcgatt gtatcaatca cgtaaagcca cgtcggatta
     3001 gtttcaatta ggtggtgttt acaacacagt ggccgatgac actttaaccc accgtggatc
     3061 cgggcgcttg ccgaagtccg gcacatgggg acacgcgagg gatatccgtg ccgctccgcg
     3121 aacgtgctcc tacgctgcgg ccttatttgt ttttcgccaa caaatgggac cccaagggcc
     3181 atattgcgaa ccaacttatc ccaaaagttg aacctatagg gcgttgagta gcgttggagt
     3241 tccgcatgaa agtcgcggcg gtgaagtttc tatgggctgt ggctgccgga ttggtcgcaa
     3301 gtgcgccgat cgcaacgatc accctgagct cggtcggcgc agctgaagaa tgtctcacga
     3361 caccgaaaaa cgaaacccca ccgggcaagc attggtatta tcgcagcgaa ggcggcacta
     3421 aacgcctttg ctggtacttg cgggaggaag gcgaaacccc ttcccagacg gcaacatcga
     3481 caccaggtcg acgaactgcg cttgaagcgg cgcccaatag ggagacgaaa ctggcgcgct
     3541 cggccgcaga cgcacatgcg gaattgccat tgccacaaac ccgcgtcgag gctgacccaa
     3601 aaatctcgcc gacgacgccg gctacgtcgt ttaatcccag aggcgctgcg cagaagctgt
     3661 caagcaatgc ctcccccgaa accgcgcggc cgccggtggc ttcgcgtagg cccgagccaa
     3721 cgggcgtgtt ctcatcgccc accgaagggc cgatatcgcc ttcgttcgtt gttgcgtcgc
     3781 cggcgccgga tactaactcg gacgcgagcg cggacacgga tttagcgccc aaggtcccac
     3841 ctgtcgtgcg gaccaaggtg gaaacgtccg cgatgggaac gcctgcctct ctccgcattt
     3901 tactgttggg gacgttcggt gcgatcgcgg ttttgtggct cacgggaagt gcggtaatgg
     3961 cccggatgcg gcgacggccg cagcgttata ctagatcgaa cccgcatacc gacttgaacc
     4021 cgccggagcg gtcgacagag gagccgacca atcacaccgg cattccgcct tcgcgcaaac
     4081 cgatgccagt aaattctccc cgccgtcgca acgcaggcag ccaatcatcg gaccgccaga
     4141 ggagcggcct aggcgacaat gcgcgtgaga tcgagcagtt gttggcgcgg ttcaccaagc
     4201 aggcgcagac cgagccgtga aagtcgcgga cttctgtttg gggtcaaaag ctgaaaggct
     4261 caacgcgagg catatgtttt ccgctttgcc cctgagaacg gacattgccg agtgcggccg
     4321 gcatgtcgga aaagtgcaag aggcggcatt ggacagcaac gcctcagatg aaagacgccg
     4381 cacctaaggc gggcttacgg cagcgccggg acgttgcatg tcccgcaata aaagtcgggg
     4441 ccaaactccg tttgcaccac cttgaagcca tcagggacgt tatcaacggt tggcatattg
     4501 gcggcttcaa attgggacag gctggcaata ccagtgttgc tgcattgcgg gcagcgtagc
     4561 gcttcattcc attgatcggt cattggcgat cctatgaagg cgagtagatt cgtcgatggt
     4621 caagaaagga cccaatcatc ggtcatgccg agagaataag agcattttgc ttgttcgtcg
     4681 gttcggtccc ggacggaaac tccatagaat tgccgttccg ggtcaaaaac ggtcgcgacg
     4741 ctcttgaaat ggaaggccgt tggcatgttt ccggccggtc gacgagcgac aaaccgatat
     4801 aagcgagttt accagacttc tcgaccagac tccggtgcta agcgccaagc ccaattgcgt
     4861 tctccctgtt cggacggagc cgcgaagtag aacagtcacg gctgagacga ctatccagac
     4921 acaaaggcca acgcagccag cgccgaaaag gccgagtgaa tcccaaaacc acaagacttg
     4981 ggatgacaaa caactcataa gggccgcgac tgtgaggcct gtaataagca gcccgagggt
     5041 tccgagattg atgctgtgga cggctcctcc accggcacat cggtgccatg gatgtgggcg
     5101 ctgttgaggc tcccacgatt cagaggagcg agccatgcag acgatcacga caatcggttt
     5161 agatatcgcc aagtcggttt ttca
//
LOCUS       DATMGX010000923         2425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_52410, whole genome shotgun sequence.
ACCESSION   DATMGX010000923 DATMGX010000000
VERSION     DATMGX010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52410"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1724
                     /locus_tag="VNR65_15090"
     CDS             231..1724
                     /locus_tag="VNR65_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ20043.1"
                     /translation="MNLGDLLHSRALENPDKTVLFCQDRTMSLEELDKSTSRLARWFL
                     DQGLQSGDRVAIHWSNRIETVQLFFGLWKAGLIAVPVNIRLKAPEISYILEHSEARMC
                     FSEPTLAQLAAEAGAGSVLSGLPRLESGDAALPEVNPDQPAVILYTSGTTARPKGAIH
                     THRTLLENSRLMVAAVPLDSCDRTLVATQMMHAVGLYMDLLPAIHSRGSAVLLPVFEA
                     AAALDAIERFRCSYFTILPALLHTAAEEQARNPRDVGSLTKLLAGGDTVPVSLQERVR
                     ILLGVEVREGYGMTEAVPLIFNSQDTIRQGSLGVAITGVQVRIVDFSDRDVPEGEIGE
                     IAVLGPGNCIGYWNDPRATETLLRNGWMHTGDLACRDSDGYYWFQGRGKEIIVRGGSN
                     ISPQEVEEALYQHPSVMEVGVIGMPDPILGERVIAFVSVRPTDAPSEKELIEFARRRL
                     ADYKTPERVLFIEQMPRGLTGKVQRRAMKEMLIAQPDLIEQCAEAGI"
     gene            complement(1674..>2425)
                     /locus_tag="VNR65_15095"
     CDS             complement(1674..>2425)
                     /locus_tag="VNR65_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWJ20044.1"
                     /translation="AARNAVELDPLSAEAHCSLAMIENAWNWNTARCEAEFHRCMELN
                     PGYALAKAKYATSCLTPLGRFDEAHEWLLRALELDPLSPNMHSDLALNYAHRGLYEQF
                     EAEAAKVIDMNPEVALKVHWYQIMSRGSRGDWAGAVAAAERAMNTAAEDSATLGFAAW
                     AYAIGGLSERAENIRNQLEQHARICYVPPMVLVTAYAPSATVDVIFALFDRALEERDP
                     WLRYTRIIFPVHRFRSDPRFRALLDKVGLSD"
BASE COUNT          493 a          704 c          745 g          483 t
ORIGIN      
        1 cctcgagcgc atcgagtggt gcgactcagc ttctctcgac ctctcagtcc gcctggcgaa
       61 tgaagccacg cccctcgaac ctccgccaga aacgtgattg gattgcctgg ttgacgttgg
      121 ccatgcattt ggaagtcgac agggggccgt cgaattgacg ggaaaccatg taacgtccgc
      181 ctatcccgtt acttcttgct cagcaggacc atacgaccta cacttgatcc atgaatttag
      241 gcgacctcct gcactcgaga gccctggaaa acccagacaa aacagttctg ttttgccaag
      301 atcggacgat gtcactggag gaactggaca aatccacttc cagactggct cgatggttcc
      361 tcgaccaggg gttgcagtcc ggcgaccgtg tggcgatcca ttggtcgaat cgtatcgaaa
      421 cggtgcagtt gttttttggc ttatggaagg ccggattgat cgcggttccg gtcaatatac
      481 gtttgaaggc gccggagatc tcgtatatcc tggagcactc agaagctcga atgtgcttca
      541 gcgaaccgac actggcgcag ttggcggcgg aagcgggcgc gggaagcgtc ttgtccgggc
      601 tgccgcgcct ggagtcgggc gatgcggctc tgccggaggt aaatccggat cagccagcag
      661 tgattttgta tacgtccggc accacagccc gtcccaaagg cgcaatccat acgcatcgca
      721 ccctcctcga gaacagcaga ctgatggtgg cggcagttcc cctcgacagt tgcgatagaa
      781 cgttggtcgc gacccagatg atgcacgcgg tcggactgta catggacttg ctgccagcta
      841 ttcattcccg cggatcggcc gtactactcc cggtgtttga ggcggcagcg gcactggacg
      901 ccattgaacg gttccggtgc agctatttca ctatcctccc agcgttgttg cataccgcag
      961 ctgaggagca ggcacggaat ccgcgggacg tgggctcgct gacgaagttg ctcgcaggcg
     1021 gagacaccgt ccctgtcagt ttgcaggaac gcgtccgcat tttactcggc gtggaggtcc
     1081 gggagggtta tggcatgacg gaggccgttc cgctcatctt caattcgcaa gacacgattc
     1141 ggcagggatc gctgggcgtc gcgataacgg gagtgcaggt acggatcgtc gatttcagcg
     1201 accgcgacgt gccggagggt gaaatcgggg agattgccgt gctcggcccg ggaaactgca
     1261 tcggatactg gaacgatccc cgggcgaccg agacgctgct acgcaatgga tggatgcaca
     1321 cgggcgatct cgcctgccgt gatagcgacg gttactactg gtttcagggc cgcgggaaag
     1381 aaatcatcgt tcggggcggt tccaacattt ctccccagga ggtggaggag gccttgtacc
     1441 agcacccgtc cgtcatggag gtgggcgtga tcggcatgcc cgacccaatt ctgggtgaac
     1501 gcgtaatcgc gttcgtttcc gtccgcccaa cagacgcccc gagcgagaaa gaactgattg
     1561 agttcgcccg gcggcgctta gccgactaca agacgccgga gagagttctg tttatcgagc
     1621 aaatgcccag gggattgacg gggaaggtcc aacggcgcgc catgaaagag atgctaatcg
     1681 ctcagcccga ccttatcgag cagtgcgcgg aagcggggat ctgaccgaaa ccggtgcacc
     1741 ggaaaaatga tgcgtgtgta tcgcagccag ggatcgcgtt cctccagggc ccggtcgaac
     1801 agcgcgaaga tcacgtcgac ggtagcggag ggtgcgtacg ctgtcaccag gaccatcggc
     1861 ggaacgtagc aaattcgcgc atgctgttcg agctggttgc ggatgttctc agcgcgttct
     1921 gagagtcccc cgattgcgta ggcccaggcc gcgaatccca gggtagccga atcctcggca
     1981 gcggtgttca tcgcccgttc agccgcggcc acagcgccag cccaatcgcc gcgggaccca
     2041 cggctcatga tctgatacca gtgcactttc agggcgacct ccggattcat gtcgattacc
     2101 ttcgctgcct cggcttcgaa ctgctcgtat aatccccgat gagcgtaatt cagcgcgaga
     2161 tccgagtgca tgttcggcga tagggggtcc aactcaagcg cgcgcagcag ccattcatgc
     2221 gcctcatcga accggcccaa aggcgtaagg cacgaagtcg cgtatttggc cttcgccagc
     2281 gcatatccag gattgagctc catgcaacgg tgaaactcgg cttcacatcg ggcggtattc
     2341 cagttccagg cgttctcgat catggccagt gagcaatgcg cctccgccga tagagggtcg
     2401 agctccacgg catttctcgc cgccg
//
LOCUS       DATMGX010000924         2849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_460313, whole genome shotgun sequence.
ACCESSION   DATMGX010000924 DATMGX010000000
VERSION     DATMGX010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460313"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VNR65_15100"
     CDS             complement(<1..572)
                     /locus_tag="VNR65_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20045.1"
                     /translation="MSPHGIAAGFSKASTYLGTWAPKGLYTRALIIIIAPIVILQSIL
                     TFVFLDRHWQAVTRRLSTATSQDIAMLADAYELMKNRSPEELDRLAEIARDRLNLSVR
                     FLPGQELPLPRPRPFFGFLDRYVSDEVRYRIHKPFWIDTLGRSDFIEIRVKLDDVVMR
                     VVAQRAQLMPSNTHIFIVWMVVSATVLLIVA"
     gene            complement(842..1561)
                     /locus_tag="VNR65_15105"
     CDS             complement(842..1561)
                     /locus_tag="VNR65_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ20046.1"
                     /translation="MTVDQALDFEMPALADDAPHILVVDDDERIRDLLGGYLRQNGLR
                     VTQAENGAAARAKLRGLAFDCIILDVMMPGESGFDIAGSLRKTSDVPILMLTARSEPE
                     HRVRGLELGVDDYVAKPFEPRELLLRLQNILRRHSSAKTKHGEASFGPFTFDLRRNEL
                     KRGEEVIRLTDKEREVLREFALSGGELVTREDLARLGEYDNERAVDVQINRLRQKIET
                     DPANPAYLHTVRGKGYVLYLD"
     gene            1737..2645
                     /locus_tag="VNR65_15110"
     CDS             1737..2645
                     /locus_tag="VNR65_15110"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HWJ20047.1"
                     /translation="MAPIPYDQRDGFIWMNGALVPWRDAKVHVLTHALHYGSGVFEGA
                     RIYDTEVFALTKHSERLHASANILGFEIPYSVAEIDQACRDVIKAQGLVEGYIRPIAF
                     RGSEEIGVAAKHARINLAIACWDWGSYFPEEERLKGIRLCMANYRRPDPKTAPSTAKA
                     AGLYMICTIEKHRSMDLGYADALMLDYRGLVAEATGANVFFIKGETIHTPTPDCILNG
                     ITRRTVMGLAKRRGIEVIERQIQPEELAGFEQCFLTGSAAEVTPVSEIGEHRFTVGEI
                     TKTLMDDYSAFARPRTRAERNLELAV"
BASE COUNT          580 a          853 c          861 g          555 t
ORIGIN      
        1 gcgacgatca gcagcacggt ggccgacacc accatccaga cgatgaaaat atgcgtgttc
       61 gacggcatga gctgcgcgcg ctgtgcgacg acgcgcatca cgacgtcatc gagcttcacc
      121 cggatttcta taaagtccga ccgtccgagc gtgtcgatcc agaacggttt gtgaatccgg
      181 tagcgaacct cgtcagagac gtaacgatcg aggaacccga aaaaaggtct cggcctcggg
      241 agcggcaact cctggccggg gaggaacctc acggaaagat tgagccgatc ccgagcgatc
      301 tcggccaagc ggtcgagttc ctccgggctt ctgtttttca tcagctcata ggcgtcggcc
      361 agcatcgcaa tatcttggga tgtcgccgtg gacagccgcc gtgtcacggc ctgccaatgt
      421 ctgtccagga acacgaaagt caggatcgac tgcaaaatga cgatgggcgc gatgatgatg
      481 atgagcgccc gcgtatagag tccctttggg gcccaggtcc ctaggtatgt cgaagctttg
      541 gaaaacccgg cggctatgcc atggggggac aatgattttt tggttggcag gggcgagttg
      601 tccgcggctt cgggagcctt gcctgcccta tccttgggcg gcgtgcgttc ggtgaaatcc
      661 agcgaaatgg ctgacgcctc cggttattca agcgatcgtc gctcaactgg gccaacgaat
      721 tgtcatcccg gccaagcgaa gcgcgagccg ggatcgcaaa aggtaggcgc ttcgatttgt
      781 tacgatcccg gataaggctt cgcctttccg ggacgacacc ggagcgtccg ccaaactatg
      841 cttagtcgag atagagaacg tagcccttgc cgcgaacagt gtgcaaatag gctgggttcg
      901 ccgggtccgt ctcgatcttc tgccgcagcc ggttgatctg aacgtccacc gcgcgctcgt
      961 tgtcatactc gccaagccgg gcaaggtctt cgcgcgtgac aagctcgccg ccgctcagcg
     1021 cgaactcgcg cagcacctcg cgctccttgt ccgtcaaccg gatcacctcc tcgccgcgct
     1081 tcagttcatt gcggcgcagg tcgaatgtga acgggccgaa cgaagcttct ccgtgcttgg
     1141 ttttcgccga agagtgacgc cgcaggatat tttggaggcg caagagaagc tcgcgcggct
     1201 cgaagggctt cgccacatag tcgtcgaccc caagttccaa ccctctcacc cggtgctccg
     1261 gctccgaccg agccgtcagc atcaggatcg ggacgtcgga agttttccgc aaggaacccg
     1321 cgatgtcgaa cccgctttcg cccggcatca tgacgtcgag gatgatgcaa tcgaaggcca
     1381 gccccctgag cttcgcgcgc gccgcggccc cgttttcggc ctgcgtgacc cggaggccgt
     1441 tctgccggag ataaccgcca agcaagtccc ggatgcgctc atcgtcgtcc acgacaagga
     1501 tgtgcggcgc gtcgtccgcc aaagccggca tttcgaaatc gagcgcttga tcgacggtca
     1561 tgtgataatg cggagcctct gccgtttgga acgggcctac ctcagaacaa cttttgactg
     1621 gacgcaagcg ctattgaggc gctttactcc ggtctccggc tttttccagc ctggagtgcg
     1681 ccgcgatttg cggcttccac taacatcaag agcaaaaaca agcattggag atggcgatgg
     1741 cccccattcc ttatgaccag agagacggtt tcatttggat gaatggggca ctcgtgccgt
     1801 ggcgggatgc gaaggtccat gtgctgacgc atgctctgca ttacgggagc ggcgttttcg
     1861 aaggcgcgcg catctacgac acggaagttt tcgccctgac gaagcattcc gagcgcctgc
     1921 atgcctcggc gaacatcctc ggcttcgaga ttccttacag cgtcgccgag atcgatcagg
     1981 cctgccgcga cgtcatcaag gcgcaaggtc tggtggaagg ctatatccgg cccatcgcat
     2041 tccgcggctc ggaggagatt ggcgtcgccg cgaagcacgc aaggatcaac cttgctatcg
     2101 cctgctggga ttggggttcg tatttccccg aggaagagcg gctgaagggc atccggcttt
     2161 gcatggcaaa ttaccgccgc ccggacccaa agacagcgcc gtcaacagcg aaggcagccg
     2221 ggctttacat gatctgcacc atcgagaagc accgctcgat ggatctgggc tatgccgacg
     2281 cgctgatgct cgattatcgc gggctcgttg ccgaggcgac gggcgcgaac gtgtttttca
     2341 tcaagggcga gaccatccac acacccacgc cggattgcat cctgaacggg atcacccggc
     2401 ggaccgtcat gggacttgcg aagcgtcgcg ggatcgaggt catcgagcgc cagatccagc
     2461 ccgaggaact tgccggcttc gagcaatgct tcctgacggg atcggcggcc gaagtcaccc
     2521 cggtttcgga aatcggcgag caccgcttca cggtcgggga aatcaccaag accctgatgg
     2581 atgattacag cgcgtttgcg cggccacgca cgcgcgcgga gcgaaatctc gaactcgcag
     2641 tctaaagcgt gttgcaatca gctggaaccg atgtcatggc cgggcttgac taggcttgac
     2701 ccgacaatcc agtctcaaaa cccaaaagat ggagtttgtt atcctggatg gccgcctcgg
     2761 agggcggcca tgacaataag gggagtccga attcgacacg cttcaggcca tttcaggtca
     2821 ccctgaagtc taccgcgacc gcgcgcttg
//
LOCUS       DATMGX010000925         3260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_418688, whole genome shotgun sequence.
ACCESSION   DATMGX010000925 DATMGX010000000
VERSION     DATMGX010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418688"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..474
                     /locus_tag="VNR65_15115"
     CDS             178..474
                     /locus_tag="VNR65_15115"
                     /inference="COORDINATES: protein motif:HMM:NF021567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HWJ20048.1"
                     /translation="MATRKEIENALRFLAPRIPDHEFGAISDHAMDSTGLRNAAPETA
                     AWLSMVSFIRHVLTEYDSLLEEGYEQESARHFVAGDMQAVLNEWGVKRPLKPSE"
     gene            complement(880..2217)
                     /locus_tag="VNR65_15120"
     CDS             complement(880..2217)
                     /locus_tag="VNR65_15120"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ20049.1"
                     /translation="MDLTAAAANGPAANLGPSPSGFTDMRGRLKAIFIGSVGNLVEYY
                     DFYAYAAFALYFAKSFFPEGDETVQQLNAAMLFAAGFLARPLGGWLFGHIADRHGRRI
                     SLTISVLLMCLGSLVIAITPTYASIGVMAPIVLAAARILQGLSLGGEYGTSATYLSEV
                     ADEKNRGFYSSFQYVTLIGGQLCAILVLLVLQQWLLTPEQLRDWGWRIPFAIGGLLAI
                     FAAVMRSNLQETESYLAAQKKRPHESSIRELFRYPREVFLVIGLTMGGTAAFYTYTTY
                     MQKFLKLSVKLTDIETTAVTGGSLVFAMALQPIYGAVSDRIGRKWLLIGFGVLGTLFT
                     IPLLTALRDAHDAWTAFLLIAAAWCIVSGYTSINAVVKAELFPAGVRATGVGLPYALT
                     VSIFGGTVESVALWFKSIGHESWFYVYLTGCIAVSLVVYLFMRDTKRQSAIRE"
     gene            complement(2673..3131)
                     /locus_tag="VNR65_15125"
     CDS             complement(2673..3131)
                     /locus_tag="VNR65_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWJ20050.1"
                     /translation="MQSSILAHRSVSPTHKETAMERKFKITVEGREYNVTVEEQTDLD
                     SNSQFEAPISFPRSAPPASTYVPAVATNTYATPQVPAEPGDVVSTMGGVVESLLVTIG
                     QEIKAGDRVIVIEAMKMKTPITANQGGKVADILVEVGEGVQTGQVLVKLA"
BASE COUNT          670 a          972 c          970 g          648 t
ORIGIN      
        1 cgtcacgttc acctttcctc aacgtttgcc gttaagttta gggccacgct cgcacgacgt
       61 cgctctgaca cgaaccggat actacatgcg catgtcaatt ctgtgaaggc ctcactttgc
      121 ctccccctgg cgtgatgata acttgatgct ctcaggagag aacacgaggt tgttacggtg
      181 gccactcgca aagagatcga aaacgctctt cgctttcttg cgccgcgcat ccccgatcat
      241 gaattcggcg ccatttcgga tcatgccatg gacagcaccg gcttgcgtaa cgccgcaccc
      301 gagacggcgg cgtggctctc gatggtcagc ttcatccggc acgtcctgac cgaatatgat
      361 tctcttcttg aagagggcta tgagcaggaa agcgcccgcc actttgttgc aggcgacatg
      421 caggccgtgc tcaacgaatg gggcgtcaag cggcccctga aacccagcga ataacaggcc
      481 tttcgcccgc ttggatatcc cagcgaacaa gaaggctttg gcgccgcgca ggatttacct
      541 ttgatgccaa cagtccgctt acgcttgcaa cgcccttcct tctcccctca cgggagaaga
      601 ggatgaaggg ggctttgagt caggttgtat ggcggttggt atgagacggg ttcaagtccc
      661 gcgtcacgtg cgtgctgcgc tgagcgctga ggcttataac gcggaagcct cgcggcaggc
      721 catgtcggaa gccgaggagg cccatctcgc ctaataaact cttgcaggaa atgattacat
      781 ccgcagccgg gaaatcgggg gacagtcgac gctccccgac ttcccagccc ggtccgcgcc
      841 gccgccagtc taggaagagg gaatgcgcct ttccgtagct cactcgcgaa tggcggattg
      901 ccgttttgtg tcccgcatga agaggtacac cacgagcgat acggcgatgc agcctgtcag
      961 gtagacgtag aaccagcttt catgcccgat ggacttgaac caaagcgcca cgctctcgac
     1021 cgtgccgccg aagatggaga cggtcagggc atagggtagg ccgacgcccg tcgcacgcac
     1081 gccggctggg aacagctcgg ccttcaccac ggcgttgatc gacgtatagc ccgaaacgat
     1141 gcaccaggcc gctgcgatga gcaggaaggc cgtccaggcg tcatgagcat cgcggagcgc
     1201 cgtcagcaaa ggaatggtga acagcgttcc cagaacgccg aacccgatca gcagccattt
     1261 gcgcccgatg cggtcggaca cagcgccgta tatcggctgc agcgccatgg cgaacaccag
     1321 cgatcccccc gtgactgccg tcgtttcgat gtccgtcagc ttgaccgaca gtttcaggaa
     1381 cttctgcata taggtcgtgt aggtataaaa ggccgccgta ccgcccatgg tcaggccgat
     1441 caccaggaag acctcgcgag gatagcggaa gagttccctg atcgagcttt cgtgcgggcg
     1501 cttcttttgc gctgcaaggt aggactcggt ctcctgcaga ttgctgcgca tgacagcggc
     1561 gaagatggcc aaaagaccgc cgatcgcaaa gggaatgcgc cagccccaat ccctcaactg
     1621 ctcgggcgtc aacagccatt gctggaggac gagcagcacc aggatggcgc aaagttgccc
     1681 gccgatgagc gtgacgtatt ggaagcttga gtagaacccc cggtttttct cgtcggcgac
     1741 ctcgctcaga taggtggcgc tggtgccgta ttcgcctccg aggctgaggc cctggaggat
     1801 gcgggcagcg gccaacacga tgggcgccat gacgccaatg ctggcgtagg tgggcgtgat
     1861 agcgatgacg agcgagccca aacacatgag caagaccgat atggtcaagg aaatgcgccg
     1921 cccatggcga tccgcgatat ggccgaacaa ccagccgccc aacgggcgag ccaggaaccc
     1981 cgctgcgaac agcatcgcgg cgttcagctg ctgcaccgtt tcatcgcctt cggggaagaa
     2041 ggacttggcg aaataaaggg caaaggccgc gtatgcgtag aagtcgtaat attcgaccag
     2101 attgccgacc gagccaatga aaatggcttt caggcggccg cgcatatcgg tgaagccgga
     2161 tggggaaggg ccgaggttag ccgctgggcc gttggcggct gcggctgtca ggtccatgtt
     2221 gcctcccaat ttcgagcggg catcgacggc tcgttaacaa acggtattgc tgcagcactc
     2281 aaaccgccgc tgcaccttga gataacgcaa tttggtcctg ctagaagttc gtttgcgccc
     2341 gggtgacgcg tgagaggcat gagccgattt tcccaaacga ccaactctgc gtccgctcca
     2401 tccaaggccg gatccaggaa ggcgacggag ataatcgaat atggcctgct ttttaccttg
     2461 ggggctgtct tgattacagc agctgtgacg ctgctgggac taaacttgtt tgcctccttt
     2521 ggtaccttgg gagacaccct taatcagccg ggtcgagttg tgagggttgc acctgctagc
     2581 tcaccggcgg cctcgacgcg cgttcgctcc tccccccgta acgccttttc aacaatcttg
     2641 ccttgggcgg acccgctgcc atagccgtcg cgtcacgcca gcttgaccaa aacctgcccg
     2701 gtctgcacac cctctccaac ctcgaccaga atatccgcaa ccttaccgcc ctggttagcg
     2761 gttataggtg tcttcatctt catcgcttcg atgacgataa cccggtcacc ggctttgatc
     2821 tcttggccga tcgtgacgag aagagactca accacgccgc ccatagtcga caccacgtcg
     2881 ccaggctccg ccgggacctg gggcgtcgcg taagtgttgg tagctactgc tggcacatac
     2941 gtagaagcgg gaggcgctga tcgcgggaaa ctgatcggcg cctcgaattg ggaattgctg
     3001 tcgaggtctg tctgctcctc gacggtcacg ttatactcgc ggccctcgac tgttattttg
     3061 aatttgcgtt ccatggcggt ttccttgtgt gtcgggctga cgctgcgatg cgccaggatg
     3121 gaagattgca tgagatccat gatgggccgc gcggccctcg gcctgccacc ccaaaccatt
     3181 gtgggcaggc tcgatgcgaa cgatgcggtg ttgcccgatc atggctgcga cagtggccgt
     3241 gatcacagcg acgtgatccg
//
LOCUS       DATMGX010000926         3096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_111800, whole genome shotgun sequence.
ACCESSION   DATMGX010000926 DATMGX010000000
VERSION     DATMGX010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111800"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            550..1818
                     /locus_tag="VNR65_15130"
     CDS             550..1818
                     /locus_tag="VNR65_15130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWJ20051.1"
                     /translation="MDPGWQAATVASTATVIDDAFNLNQRLRMMMRFRENVPALVRPM
                     LVTGIALMLLACEDREPSRASSPSSPPEVGVVTVSASPVTLTKELPGRITPMRIAEVR
                     PRVSGIIVERTFQQGSTVKAGDILYRIDPAPLQVELEKARAALAKAEAVLYQADRQED
                     RLRTLLNGQTTTQAQYELALATERQAAAEVAAQKAEVSQTQLNLDYATVRAPINGRIG
                     RALITEGALVGKNEATHLATVQQLDPIYADFTQSVTEVNALKRDLALGRLESVSPDTA
                     KVHLLLEDGSVYEHPGRLLFSDVTVDPGTAKVTLRGEFPNPKFELLPGMYVRVQIEEG
                     IATSGITVPAQAVQRTDAGTSAVYVVNNEGRAVLQLVRAGRTLGNEVLIEDGLKPGYR
                     VVVDGFQKFTPGTLVKAVVLNEASAETVSN"
     gene            1859..>3096
                     /locus_tag="VNR65_15135"
     CDS             1859..>3096
                     /locus_tag="VNR65_15135"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWJ20052.1"
                     /translation="MSSFFIPRPVFAWVVAIFICLAGVLSLPFLPIAQYPIIAPPSIS
                     ITTVYPGASTQNLYYSVTRLIEEELNGASNILNYESTSDTTGEVEIVANFKPGTDIEL
                     ASVDVQNRIKRIEPRLPEPVRKEGIFITEASSAILQFITLSSSDGSLDEVALGDIATR
                     YVLPEIRRVPGVGKARLFSTERAMRIWLDPDKLLGLNLTADDVQAAIAAQNAQVASGL
                     LGIQPSPKDQPVQNMVLVRGQFESPEEFGDIILKANPNGSNVRLRDVARLEVGGLSYS
                     FSTRLNGKPAAAVAVQLAPTGNALATADTVKAKMTELASFFPPGVKYDVSYDITPVIQ
                     ASVKKVLMTLAEAVVLVFVVMYLFLQNIRYTLIPTIVVPIALLGTCATLLIMGFSINV
                     LTMFGMVLAIGILVDDAIVVV"
BASE COUNT          638 a          933 c          904 g          621 t
ORIGIN      
        1 tggacgattt ggcgggtggg agcgtcccca caacttctga ccggatccta ttcggcgccc
       61 gttcaaagaa gaaacgcgat aagtgagatc gacggtcgcg aggggtcgcg acctgacatg
      121 ccggttttct agcaattccg tgcgcgtcta ggcggcttcg agatccgaat gcggtcttct
      181 ccggcaaggc caaaagaatc ccgcgcctga cccataagag acatgaccga ccgtggaatt
      241 agtctcatgg acaaaagcgg tcgcagcgcc tgatctctgc aatcggaaat ttggcccgga
      301 ccggctttcg cgagagatgc atggcaccac cgagcgccac gacattcgtt cgaaatggac
      361 gggacgagag gggactactc tttgcccgac aggttccgac gtgctccggc aagcaagtat
      421 tatccctggg cacttgtacg gaggaaacca ctcctgtggg tggctcaatt ctcattcccg
      481 tgaaccatat caggggtcct ggacacggct ttgcgtcttg ccgcttgccg aaatcacgtg
      541 ttcgtgaaca tggacccagg atggcaagcg gccactgttg cgagcacggc cacggtcata
      601 gatgacgcct tcaaccttaa ccaacgcttg aggatgatga tgaggtttcg ggaaaatgtc
      661 cccgcgcttg tacgcccgat gctcgtgacc ggcatagcat taatgctcct ggcctgcgag
      721 gacagggagc cctcgcgggc atccagccct tcaagccctc ccgaagtcgg ggtcgtcacc
      781 gtatccgcat cgcccgtcac actcacaaag gagttgccgg gccgcattac tccgatgcgc
      841 atcgcggaag tgcgtccacg cgtttccggc attatcgtcg aaaggacgtt tcaacaaggc
      901 agcaccgtaa aggctggcga catactctac cgaatcgatc ctgccccgtt gcaggtcgaa
      961 ctcgagaaag cgcgtgcggc gctggccaag gcagaagcgg ttctgtatca agccgatcgc
     1021 caggaagacc ggctgcgtac acttctgaat gggcagacca caacgcaggc gcaatatgag
     1081 cttgcgctcg ccaccgagcg gcaggccgca gcggaagtgg cggcccagaa agcggaggtg
     1141 agccagacgc agctgaattt ggactacgcg acggtgaggg cgccgatcaa cggccgcatc
     1201 ggccgcgcgc tgattacaga gggagcgctc gtcggcaaga acgaagccac tcaccttgcg
     1261 acggtccagc agctcgatcc gatttatgcc gacttcaccc agtcggtgac cgaggtgaac
     1321 gcgctcaagc gtgatctcgc gcttggccgc ctcgagagcg tatcgccgga cacagccaaa
     1381 gtgcacctac tgttagagga tggttcggtt tatgagcacc ccggccgcct ccttttttcg
     1441 gatgtcaccg tagaccccgg cacggcgaag gtgacgctgc gtggtgagtt ccccaatccg
     1501 aaattcgagc ttctgcccgg catgtacgtt cgcgtgcaga tcgaagaagg catcgcgacc
     1561 tcaggcatta ccgtgcccgc ccaagccgtt cagcgcacag atgcgggcac cagcgcggtc
     1621 tacgtcgtaa acaatgaagg gcgtgccgtc cttcagctgg tgcgagcggg tcgaacactc
     1681 ggaaatgagg tgctgataga ggacggcttg aagcctgggt atcgagtcgt cgtcgacgga
     1741 tttcagaaat tcacgccggg tacgctcgtg aaggctgtgg tgttgaacga agcctctgcc
     1801 gaaaccgttt cgaactgagc ccagccccaa cttaaagcct gcccggagaa cgacaaacat
     1861 gtcgagcttt tttattcccc gcccagtctt tgcctgggta gtggctatat tcatctgtct
     1921 ggccggtgtg ctttcgctgc cctttctgcc gattgcgcaa tacccgatca ttgcgcctcc
     1981 ctcaatctcg atcacgaccg tctaccctgg cgcatcgacg caaaacctct attacagcgt
     2041 cacccggctc attgaggagg agctaaacgg ggcgtccaac atcctgaact acgagtcgac
     2101 gagcgacacg accggtgagg tggagatcgt cgccaacttc aagccgggca cggacatcga
     2161 actcgcctcc gtcgacgtgc agaaccggat caagcgcatc gaaccgcgcc tgcccgaacc
     2221 ggttcgcaaa gaaggcatct tcattaccga ggcgagcagc gccatcctgc agttcatcac
     2281 cctctcttcg agcgatggca gtcttgatga ggtggcgctt ggcgacatcg caacccgcta
     2341 tgttctgccc gagatccgcc gcgtgcccgg tgtcggcaag gcgaggttgt tctcgacgga
     2401 gagggcgatg cgaatctggc tcgatcccga caagctcctt ggtctcaacc tcacggccga
     2461 cgatgttcag gcggccatcg cggcgcagaa tgcgcaagtg gcgtcaggcc tcctcgggat
     2521 acagccgagc cccaaggacc agcctgtgca gaacatggtg ttagtcagag ggcagttcga
     2581 atcgccggaa gagttcggag atatcatcct caaggccaac ccgaacggct ctaacgtccg
     2641 cttgcgcgat gtcgcgcgct tggaggtcgg cggcctgtcc tattcatttt ccacgcggct
     2701 caatggcaag cctgctgctg cggttgccgt gcagcttgcg cccacaggta atgcgctcgc
     2761 gacggccgac actgtgaagg cgaagatgac ggaactcgct tcgttcttcc ctccgggcgt
     2821 caagtatgat gtctcctacg atatcacgcc agtgattcag gcttcggtaa agaaagtgct
     2881 gatgacgctt gccgaggccg tcgtgcttgt cttcgtcgtc atgtatctct tcctgcagaa
     2941 catccgctat acgctaattc ccaccatcgt ggttccgatt gcgcttctgg gcacctgcgc
     3001 caccctgttg atcatgggct tctcgatcaa cgtgctgacc atgtttggga tggtgctcgc
     3061 aatcggcatc ctcgtcgacg acgccatcgt ggtggt
//
LOCUS       DATMGX010000927         4226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_10095, whole genome shotgun sequence.
ACCESSION   DATMGX010000927 DATMGX010000000
VERSION     DATMGX010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10095"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..862
                     /locus_tag="VNR65_15140"
     CDS             join(108..429,429..862)
                     /locus_tag="VNR65_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611877.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWJ20053.1"
                     /translation="MQRYALRDDQWTKIKDFLPGRVGHVGGTAADNRLFVDAILYRYR
                     TGIPWRDLPERYGDWKATHKRLRRWCESGVFSRILKALAFDADAEYMSIDSTSVRAHQ
                     HSAGAPKKGGEDQAIGRSRGGLTTKIHAIVDALGNPVALSLTPGQAADITQAVALLDQ
                     VEPEAFLADKGYDSDALIETLKERGITPVIPSKANRREARKTDFALYRERNLVERFFN
                     KLKQYRALATRYDKLANTFLAAVALVCVLLWLN"
     gene            1192..3006
                     /locus_tag="VNR65_15145"
     CDS             1192..3006
                     /locus_tag="VNR65_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWJ20054.1"
                     /translation="MRLTIKVKLAIAFTFLAVLAGCVSLLGISSLESFKTTMAHLIRG
                     PIARLKLTEELRVAAIHVGMAEKNMLIASSPEEVIRYRTEMQKRRNSFMAQYENALNI
                     ATDEGRQKLEAVTPLWMRWNAALDQVGDLMERGHQPEAKSLAMGESRDLRLEIQRRFE
                     EIAKTEERAGQAATTNADAQYDRAWQLLAGAAIAAFLISIISGVLLSFSIGRGLRRAV
                     SLADAVALGDLHREVKPASNDEIKDLIDSLNRMTANLRATAEMAEAIANGDLSVEPKP
                     LSDKDMLGIALQCMTGKLREVVAGALAAAGNVSSGSEQMSAAIQELSAGANDQAASAE
                     EVSSSMEQMAANIKQNADNATQTEKIARQSSADAQASGVAVNRAVQAMQTIAEKIAFV
                     QEIARQTDLLALNAAVEAARAGEHGKGFAVVASEVRKLAERSQTAAAEISALSGQTMT
                     TAREAGAMLAKLVPDIRKTAELVEEISAACREQDIGANQVNQAIQQLDKVIQQNAGAA
                     EEMSATSEALSGQAETLQTSIAFFRIGDAGHGGAATPAAPASTVKSPAKPKAAVVAKR
                     PSSAAKARPVHASRAGNGVALNLSNGSADVLDADFERY"
     gene            3022..3522
                     /locus_tag="VNR65_15150"
     CDS             3022..3522
                     /locus_tag="VNR65_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWJ20055.1"
                     /translation="MTKPAARSAAEEGQYVTLGIEHEVFAVPVESVLEILDMRRFCRI
                     PEAPSYMLGLLDLRGRSVPVLDLRAKLGLPSQSATDTSRILVIEVIMNGRPLVLGLVA
                     DRVIEVLAMNSSEIEPPPEIGVRWRSQYISGVGHRGENFIIIFDLPRLFSTEDATAIQ
                     KSVGAS"
     gene            3573..>4226
                     /locus_tag="VNR65_15155"
     CDS             3573..>4226
                     /locus_tag="VNR65_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HWJ20056.1"
                     /translation="MPRPGTSPGSAEHLSARDFDRLGQFIQLRSGIKMPAEKKTMLES
                     RLRRRILALGKTSLAEYCHYLFEEGRLDEEETNLIDAVTTNKTDFFREGEHFRFLTEN
                     AVPLLLAERSELRRSPLKVWSAACSNGAEPYTLAMVLSELRDAIGAFTILATDICSEV
                     LKTAVRGIYPASMAEPIPHDLRRRYLLRSKESAADSVRVVPDLRRSVQFGRLNLIAPP
                     "
BASE COUNT          888 a         1310 c         1286 g          742 t
ORIGIN      
        1 cgccggatca agaatcgtgc gcggcctttg ttgccgtcgt cagtctaggg attcccaaat
       61 cactttttct ctgactcatg acctgtcggc aatggggagg ccgacagatg caacgctacg
      121 ccttgcgcga cgaccaatgg acaaagatca aggactttct gccgggtcgg gtgggccatg
      181 tcggcggcac ggcggcggac aaccgcttgt ttgtcgatgc catcctttat cgataccgaa
      241 ccggcattcc atggcgtgat ctgcccgagc gctatggcga ctggaaggcg acgcacaagc
      301 gcttgcgcag atggtgcgaa agtggcgttt tttcccgcat tctcaaggct ttggcgtttg
      361 atgccgatgc ggagtacatg tcgatcgatt caaccagcgt ccgcgcccac caacatagcg
      421 caggcgccca aaaaaaggcg gcgaagatca agccatcggc agatcccgcg gcggcctgac
      481 gaccaagatc cacgccatcg tcgatgcgct cggcaacccc gtggcgctgt cgctcacgcc
      541 gggccaagcg gccgatatca cacaggcggt cgcgttgctc gaccaagtcg agcccgaagc
      601 ctttctcgcc gataagggtt atgactccga cgcgttgatc gagacgctca aggagcgtgg
      661 catcacgccc gtcatcccct cgaaagccaa tcgtcgcgag gcgcgcaaaa ccgatttcgc
      721 cctctaccgt gagcgcaatc tcgtcgagcg cttcttcaat aagctcaagc agtaccgcgc
      781 tctcgccacc cgctacgata agctcgccaa cactttcctt gccgccgtcg ccctggtttg
      841 cgtactcctc tggctcaact gacgacaggc gctaatgcgg tcttccccgc aaaggctaaa
      901 atagaactcc tagaacgccc tccacttcgg gttattttga taagagcgcg atcgatgggc
      961 ttcttgagag ggctatgatc cggcagcgcg atagctgaaa ttaactccgt ttgaaaagcc
     1021 ctcagcgttc gcgctgtgcc cgccgtccaa cttttcccgc acgaacgact tgttaggttc
     1081 aattttgctc gagactgtaa cggcatccca atctgaatag gacaaagctc aagctctccc
     1141 taccagcggg cgaatcgcgc cttggcctag cgagggaaag gacgaggaat catgcgccta
     1201 acaataaagg tcaagctcgc gattgccttt acgtttcttg ccgtgctggc gggatgcgta
     1261 tcattgctcg gcatcagcag cctcgagtcc ttcaagacga cgatggcgca tctcatccgc
     1321 ggtcccatcg cccggctgaa actcaccgaa gagctgcgcg tggccgctat ccatgtcggc
     1381 atggcggaga agaacatgct tatcgcaagt tcgccggagg aagtgatccg ctaccggacc
     1441 gagatgcaga agcggcgcaa ctcgttcatg gctcagtatg aaaacgcgct gaacatagcg
     1501 acggatgagg gacggcagaa gctcgaagcc gtaactccac tgtggatgcg gtggaatgcg
     1561 gcgctggatc aggttggaga cttgatggag cgcggccatc agcccgaggc aaagtcgttg
     1621 gccatgggcg agagccggga tttgaggctt gaaattcagc ggcggtttga ggaaatcgcg
     1681 aaaacggagg agcgggcggg tcaagccgcg actacgaacg ccgacgctca atacgaccgc
     1741 gcctggcaac tgctggccgg cgcagctata gcggcgttct tgatctccat catctccggc
     1801 gtcctcctct cattcagcat cggccgcggc ttgcgccggg cggtttcgct cgccgacgcg
     1861 gtagcgctcg gcgacctcca tcgggaggtg aagcccgcga gcaacgacga gatcaaggat
     1921 ctcatcgact cgctgaaccg catgacggcg aacctgcgcg ccaccgccga gatggccgaa
     1981 gccatcgcca acggcgacct ttccgtagag ccgaaaccct tgtccgacaa agacatgctc
     2041 ggcatcgcgc ttcagtgcat gaccgggaag cttcgcgaag tggtcgccgg cgccctcgcc
     2101 gccgccggca acgtgtcatc aggcagcgag cagatgtccg ccgccatcca agagctttcc
     2161 gcaggcgcca acgatcaagc cgcctcggcc gaagaggtgt cctcctcgat ggagcagatg
     2221 gccgccaaca tcaagcagaa tgccgacaac gccacccaga ccgagaagat cgcgcggcag
     2281 tcgtcagccg atgcgcaggc gagcggagtc gccgtcaacc gcgcggtgca ggcgatgcag
     2341 accatcgccg agaagatcgc cttcgtacag gagatcgcgc ggcagaccga tttgctggcg
     2401 ttgaacgcgg ccgtggaggc ggcgcgcgcg ggcgaacacg gcaagggctt tgcggtggtg
     2461 gcctctgaag tcaggaaact cgcggagcgt agccagacgg ccgccgctga gatcagtgcg
     2521 ctgtccgggc agaccatgac gacggcgcgc gaggcgggcg cgatgctcgc caagctcgtg
     2581 cccgatatca ggaagacggc cgaacttgtg gaggagattt cggcggcctg ccgcgagcag
     2641 gatatcgggg ccaaccaggt gaaccaggcc atccagcagc tcgacaaggt gatccagcag
     2701 aatgcgggcg cggctgagga aatgtcggca acttcggagg cgctctcggg ccaggccgag
     2761 acgctgcaga cgagcatcgc gttcttccgg atcggcgatg cgggccacgg gggtgcggca
     2821 accccggcgg cgcccgcgtc gaccgtaaag tcacccgcga agccgaaagc ggccgtggtg
     2881 gcaaaaaggc cgtcctcggc ggcaaaggcc cggcctgtcc atgcaagccg ggccggcaat
     2941 ggcgtcgccc tcaatcttag caacggcagc gccgacgtcc tcgacgccga cttcgaaagg
     3001 tactgagcga tgaacctcgc gatgacaaaa ccagcggcga ggtccgccgc tgaagagggg
     3061 cagtatgtaa ccctcggcat cgagcatgaa gtgttcgccg tgccggtgga gtcggtgctt
     3121 gaaattctcg atatgcgccg cttctgccgg atacccgaag cgccgtccta catgctggga
     3181 ctgctcgacc tgcgcggacg aagcgtcccg gttctcgatc ttagggccaa gcttggcctg
     3241 ccttcgcaaa gcgccacgga tacctcccgc atcctggtta tcgaagtgat catgaatgga
     3301 cgccccctcg ttctcggcct cgtcgcggac cgggtcatcg aggtgctcgc catgaacagc
     3361 agcgagatcg aaccaccgcc ggagatcggc gtgcgctggc gatcccagta tataagcggc
     3421 gtcgggcacc gtggcgaaaa cttcatcatc atcttcgatc tgccgcgtct gttctcgacc
     3481 gaggacgcaa ccgcaattca gaaatcagta ggagcgtcat gagtcttgcg ttgaggaaga
     3541 cggattttga aagtcctgta aggcgtgccg ccatgcctcg tcccgggacg agtcccggaa
     3601 gcgccgaaca tctgagcgcg cgcgactttg accgcctcgg gcaattcatc caactcagaa
     3661 gcggcatcaa aatgccggca gaaaagaaaa ccatgctgga atcccgcctg aggagacgga
     3721 tactagcatt gggaaagacg tcccttgccg aatattgtca ttaccttttt gaggagggca
     3781 ggcttgatga ggaggaaacg aatctcatcg atgccgttac aaccaacaag accgattttt
     3841 tccgcgaagg ggagcacttc cgttttctga ccgaaaacgc ggtgccattg ctcctcgccg
     3901 agcgcagcga gctgagacgc tcgccgctca aggtctggag cgcggcgtgc tcgaacgggg
     3961 cggagcctta cacgctggca atggtcctgt ccgagcttag agacgccatc ggcgccttca
     4021 ccattctcgc gacggacatc tgctccgagg tcctgaaaac ggcggtgcgc ggcatctacc
     4081 ccgccagcat ggccgagccc ataccgcacg acctcagacg acgctacctg ctccgatcaa
     4141 aggaaagcgc ggcggacagc gtgcgcgtcg tcccagacct tcgccgcagc gtccagttcg
     4201 ggcggctcaa cctcatcgcg ccccca
//
LOCUS       DATMGX010000928         2409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_348848, whole genome shotgun sequence.
ACCESSION   DATMGX010000928 DATMGX010000000
VERSION     DATMGX010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348848"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VNR65_15160"
     CDS             complement(<1..380)
                     /locus_tag="VNR65_15160"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWJ20057.1"
                     /translation="MKVKKPNDKPEPNVFVIFGGAGDLTWRKLVPALFDLSQDRSMPD
                     NFSVIAVDRVDLTDEKLRRRLREGVRRFSRQGKVRSGNWGDFARHIHYQKGDFKKLET
                     YTALGEQCAKLEKEWGEKVHRVFY"
     gene            complement(377..1804)
                     /gene="gndA"
                     /locus_tag="VNR65_15165"
     CDS             complement(377..1804)
                     /gene="gndA"
                     /locus_tag="VNR65_15165"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HWJ20058.1"
                     /translation="MTNKQAQQQYEIGMVGLGVMGRNLVLNMADHGFLVAGYDKDQTK
                     VEALRKESADLTVRGATSIIDFIALLRKPRAIMMLVPAGAPVDSVIKNLLPHLEKGDL
                     IIDAGNSYFKDTDLRARNLAAESIQFLGVGVSGGEEGARHGPSIMPGGPKEAYERVRP
                     VFEAAAAKVNGDPCVTWLGPGSAGHFVKMVHNGIEYGVMQLIAETYDMMKRGMGLNDD
                     KIQEVYASWNKGELNGYLVEITGHIFSRKDEKTGNRLIDEILDVARQKGTGMWTSQSA
                     MELQVPTPTIDVAVAMRDLSVFADERKQAGAIYQRLLRPLTDDRDKLLTQLGRALYAG
                     MITIYAQGMALLLVASDKYGYHLDPEAVARIWRGGCIIRAALLEDICEAFRARRDLPN
                     LLLDPGLSQKLIQHQEDLRQVVCQAAVSGVPIPGLMASLGYLDAYCSVWLPANLIQAQ
                     RDYFGAHTYERIDARGMFHTEWGKA"
     gene            complement(1815..>2409)
                     /locus_tag="VNR65_15170"
     CDS             complement(1815..>2409)
                     /locus_tag="VNR65_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HWJ20059.1"
                     /translation="VFSFKQPSAEELKHDFLWRTTRHLPERGRIGIFNRSYYEEVLVV
                     RVHPETLRGQGLPDESLDEKTIWEERYRSIVDLEEHLHRNGTRIIKFYLHLSKEEQRK
                     RFLERIDEPEKSWKFSLSDIHERKFWQDYVKAYEACLNATSTPHSHWYVVPADDKENA
                     RLIISQIVLDALNGLKMAYPKITEKRLRELKSIRKQL"
BASE COUNT          514 a          734 c          593 g          568 t
ORIGIN      
        1 atatagaaga cacggtggac tttctcaccc cattcttttt ccagtttcgc gcattgttcg
       61 cccagggccg tatacgtttc cagcttcttg aaatcgccct tctggtagtg gatatgcctg
      121 gcaaagtcgc cccaattgcc acttctcacc tttccctggc gcgaaaacct cctgacgcct
      181 tcgcggaggc gccggcgcag cttttcgtcg gtcagatcta cccgatccac cgcgataacc
      241 gaaaagttgt ccggcatact cctatcctgg gagaggtcaa acagggctgg cacgagcttc
      301 cgccaggtca ggtctccggc tcccccaaat atgacgaaca cattgggctc aggcttgtca
      361 ttaggttttt tgaccttcac gccttccccc attcggtgtg aaacatcccc ctggcatcta
      421 tcctttcata ggtgtgggct ccgaaatagt cgcgctgggc ctggatgaga ttggccggca
      481 gccaaacgct gcaataggcg tcaagataac ccagcgaagc cattagcccc gggatcggca
      541 cccctgacac agccgcctgg cagaccacct ggcgcaaatc ttcctgatgc tgaatcaact
      601 tctgcgacag gcccggatcc aggagcaggt tcggcagatc gcgcctcgcg cgaaacgctt
      661 cacagatatc ttccagcaac gcagcgcgta tgatgcagcc gccccgccaa atccgcgcca
      721 cggcttcagg atcgaggtga tacccgtatt tgtctgaagc gaccagcagg agcgccatac
      781 cctgcgcgta tatggtaatc atcccggcat agagcgcacg gcccagttgc gtgaggagtt
      841 tgtcacggtc gtctgtaagg ggccgcaaca atcgctgata gatcgcaccg gcttgtttcc
      901 gctcgtctgc gaacacggac aaatcccgca tcgcaaccgc gacgtcgatt gtcggagtcg
      961 gcacctgaag ctccattgcg ctctgcgagg tccacattcc cgtccctttc tgcctggcca
     1021 cgtccaggat ttcatcgatc aatcgattac ctgtcttctc gtcctttctg gagaatatgt
     1081 ggcctgtgat ctccacaaga tagccattga gctcgccctt gttccatgaa gcatagacct
     1141 cctggatttt atcatcgttc agacccatac cacgtttcat catgtcgtac gtctcggcga
     1201 tcaattgcat gacgccatat tcaatgccgt tgtgcaccat cttcacgaag tgcccggcag
     1261 atcccggacc cagccaagtg acgcaaggat caccgttgac cttcgccgca gcggcttcaa
     1321 acaccggacg gacccgctcg taggcctcct tcggaccgcc cggcatgatg ctcggtccgt
     1381 gacgcgcgcc ttcttcgcca cccgacacgc ccacgcccag aaattgaata ctttccgccg
     1441 ccaggttgcg ggcgcgcaaa tccgtgtctt taaagtagga attgccagca tcaatgatga
     1501 gatcgccctt ctccaaatgc ggcagcagat tcttgatgac cgaatccacc ggagcgccgg
     1561 cgggcaccag catcatgatt gcgcgcggtt tacgaagcag tgcgatgaaa tcaatgatgc
     1621 tcgtcgcgcc gcggacggtg aggtccgccg attccttccg cagcgcctca accttggtct
     1681 ggtctttgtc ataacctgcc accaggaaac catggtcggc catgttaagc acgagatttc
     1741 gtcccatgac gcccaagccg accatgccga tttcatactg ttgttgagct tgcttattag
     1801 tcatggtttt accttcagag ctgtttacgt attgatttca attctcgcaa acgtttttcg
     1861 gtgatcttgg gatatgccat ctttagtcca ttgagcgcat cgaggactat ttgggaaata
     1921 atcaaccggg cattctcctt atcatcggcc ggaacaacat accagtgcga gtgaggggtg
     1981 cttgtcgcat tcaggcatgc ctcataagcc ttcacgtaat cctgccagaa ttttctctca
     2041 tgaatatccg agaggctgaa tttccagctc ttttccggct catcaatgcg ctccagaaat
     2101 cgctttcgct gctcctcctt cgacaggtgg agataaaact tgatgatccg ggtgccgttt
     2161 cggtggagat gctcctccaa gtcgacaatg gaacgatacc gctcctccca aatggtcttc
     2221 tcgtcaagcg actcatccgg aagaccttgt ccgcggagcg tctccggatg cacacgaacg
     2281 acgagcactt cctcataata ggaacgattg aagatgccga tacgcccgcg ttccggcaga
     2341 tgacgagtcg ttcgccaaag aaaatcgtgt ttcagctctt cagcgctcgg ctgtttgaaa
     2401 ctgaaaacc
//
LOCUS       DATMGX010000929         3428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_116867, whole genome shotgun sequence.
ACCESSION   DATMGX010000929 DATMGX010000000
VERSION     DATMGX010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116867"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VNR65_15175"
     CDS             <1..616
                     /locus_tag="VNR65_15175"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HWJ20060.1"
                     /translation="VYYIGPMFTAFRGTAEGVAYYRTLHEEVEERLRSGLGPLTPEGS
                     QEKERYRLVMEGPPNWTSFREFWKMFYDEGAVVVASSYTKVGGLYDGGFRHDPARPLE
                     TLAEYCMGCYTNLGLPTRVELLERYIREYRADGFMINSIKSCNSFSVGQLLMLRQLEQ
                     RTGVPGGFIESDLVDPRYFSAANIKNRIESYLQMLEQKRAGANP"
     gene            613..1944
                     /gene="bcrA"
                     /locus_tag="VNR65_15180"
     CDS             613..1944
                     /gene="bcrA"
                     /locus_tag="VNR65_15180"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit A"
                     /protein_id="HWJ20061.1"
                     /translation="MSTLRVHVGIDLGSTTTKAVVLNDAGEVIGRGITNSRSNYDVAC
                     QVVREEAMVRTRFTLTRQAIGGDPALAQVEKAFLSSLQRNFRFRQHLDQLARLREALL
                     RATGDARHSAHRSELTAALESILDGMVERTAVHFSELAVRKSDFFRDLATADYMQLAE
                     QHSDSRGITFDMLCGLFDAAILVVENSTGQGSFETQLRGALEPTLASATRAVQEQAPR
                     LRAVVEATCRAEIVEAGTVGTGYGRQRLPFPKEQIRSEILCHGLGAHAMFPGTRTVLD
                     IGGQDTKAIQVDADGIVVSFQMNDRCAAGCGRYLGYIADEMNLGLHELGPLAQQSQRR
                     VKINSTCTVFAGAELRERLSLGQNREDILAGLHRAIILRAMSLLARSGGVSDEFTFTG
                     GVARNPAAVDALRQLVQENYGERTLNISADSIYTGALGAALFARRSAQANP"
     gene            1941..2819
                     /gene="bcrD"
                     /locus_tag="VNR65_15185"
     CDS             1941..2819
                     /gene="bcrD"
                     /locus_tag="VNR65_15185"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HWJ20062.1"
                     /translation="MTTTTIASRTRSAEGQITAGVDVGSSAVKIAVLLTTSGGETEVL
                     TLLTERIRRRELRKVVNEGYEAALRAAGVAAQEVCYVASTGEGELVDFRRGHFYGMTT
                     HARGAIFLMPEVRAVLDVGALHARAMRIDERSRVLSYRMTGQCASGSGQFLENIARYL
                     GIALQDVGSLSCGAGNPQVVSSICAVLAETDVINMVSRGISTPDILKGIHLSMAGRYL
                     KLLRAIDVHGQVAITGGLSADTGLAQALQESARKDGLDVEIRTHPNAIYAGALGAALW
                     GAYRHRMLAGQVEVSQ"
     gene            2816..>3428
                     /locus_tag="VNR65_15190"
     CDS             2816..>3428
                     /locus_tag="VNR65_15190"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ20063.1"
                     /translation="MTSAKELAGKTAVVTGGSGGIGSAIVRTLQQAGAVATSLDLSPP
                     PDNDIPWLHCDVRSDDSVGSAVQQFFQQNGRLDLVIHAAGISREAVVWKLTVEDWDAT
                     QAVNLRGAFLLLRHAIPLMRQGQGGRVVLIGSINGSRGKFGTSAYSASKAGLLGLAKS
                     VARETGRFGILLNVIEPGWVRTPLTEALPAAIRQAAEAETLLGS"
BASE COUNT          638 a         1038 c         1106 g          646 t
ORIGIN      
        1 tgtgtattac atcgggccca tgttcaccgc gtttcgtgga acggccgaag gtgtggccta
       61 ctaccgcacg cttcacgaag aggtagaaga gcgtctgcgc tcaggtttgg gtccgctgac
      121 gccggaagga tcgcaggaga aggaacgcta ccggctggtg atggaaggtc ctcctaactg
      181 gaccagcttc cgcgagttct ggaagatgtt ctacgacgaa ggcgcggtgg tagtggccag
      241 cagctatacc aaggttggag ggctgtacga cggcggcttc cgccacgatc ccgcacgccc
      301 cttggagacg ctggcggagt attgcatggg ctgttacacc aatctcggct tgccaacgcg
      361 tgttgaactg ctggagcgct acatccgcga gtatcgtgcc gacggattca tgatcaacag
      421 catcaagagc tgcaattcct ttagcgtggg ccagctcctg atgctgcgcc aactggagca
      481 gcgcaccggc gtccccggcg gctttatcga aagcgacctg gtggacccac gttacttctc
      541 cgccgcgaac atcaagaacc gcatcgagag ctatttgcag atgctggagc agaagcgtgc
      601 gggcgcgaac ccatgagtac actgcgggtc catgtcggca ttgatctggg ttccactacc
      661 acgaaggcag tcgtcctcaa cgacgccggc gaggttatcg gccgcggcat caccaacagc
      721 cgcagtaact acgatgtcgc ctgccaggtg gtgcgggagg aagcgatggt ccgcacccgc
      781 ttcacgctca ctcggcaggc catcggcggc gatccggccc tggcccaggt cgagaaagct
      841 ttcctttcct cgctgcaacg aaacttccgg tttcggcagc atctcgatca gctcgcgcga
      901 ctgcgggaag ctctgctccg cgccaccggc gatgcgcgcc acagcgctca tcgtagcgaa
      961 ctaactgctg cgctcgaaag catcctggat gggatggtcg agcggaccgc ggtccacttc
     1021 agcgaattgg cggtgcgcaa gagcgacttc ttccgcgatc ttgccaccgc cgactacatg
     1081 cagttggcgg agcagcacag cgattcacgt ggaatcacct tcgacatgct atgtgggttg
     1141 ttcgacgcgg cgatcctggt ggtggagaac tcgaccggtc agggtagctt cgaaacacaa
     1201 ttgcggggcg cgctggagcc tactctggcg tcggcgacga gggcggtgca ggagcaggcg
     1261 ccgcggctgc gcgccgtggt ggaggcgacc tgtcgcgccg agatcgtaga ggccggcacc
     1321 gtgggcacag gatatggccg ccagcgcttg cctttcccca aggaacagat tcgcagcgag
     1381 attctttgcc acggactggg cgcgcatgcc atgttccccg gcacccggac ggtgctggac
     1441 atcgggggac aggacaccaa ggcaatccaa gtggacgccg acggaatagt tgtcagcttc
     1501 cagatgaatg accgctgcgc tgccggatgc gggcgttacc tggggtacat cgccgatgaa
     1561 atgaacctgg ggttgcatga gttgggaccg cttgcccaac agagccagcg ccgggtgaag
     1621 atcaacagca cgtgcacggt ctttgccggt gcggagttgc gagagcgcct ctccctcggc
     1681 cagaatcgcg aggacatact ggcgggactg catcgcgcca tcattttgcg cgcgatgtcg
     1741 ctgctggcgc gctccggagg tgtcagcgat gagttcacgt tcaccggcgg ggtggcccgc
     1801 aatccggcgg ccgtggacgc actgcgccag ttggtgcaag agaattacgg cgagcggacg
     1861 ctgaacattt ccgccgattc gatttacacc ggcgctctgg gcgcagctct gttcgcccgg
     1921 cgtagcgcac aggccaaccc atgacgacca ccaccattgc gtcgaggact cgatctgcgg
     1981 aggggcagat taccgctggc gtcgatgtgg gttccagcgc ggtgaagatt gccgtactgc
     2041 tcactaccag cggcggtgag accgaggtgc ttaccctcct caccgagcgc atacggcgcc
     2101 gggagttgcg caaggtagtc aatgagggat acgaggcggc actccgcgcg gctggcgtgg
     2161 ctgcgcaaga ggtttgctat gtggccagca ccggcgaggg cgagttggtg gattttcgcc
     2221 gaggacactt ctacggcatg accacccacg ctcgcggcgc catttttctt atgcccgaag
     2281 tccgcgccgt tcttgacgtc ggggccttgc acgcgcgcgc catgcgtatt gacgagcgca
     2341 gccgcgtgct cagctaccgc atgaccgggc aatgcgcttc cggctccggc cagttcctgg
     2401 aaaatattgc ccgctacctg ggtatcgctt tgcaggacgt tggctccctc tcctgcggcg
     2461 ccggcaatcc acaagtggtc agcagcatct gcgccgtcct ggcggagacc gatgtaatca
     2521 acatggtcag ccggggaatc tccacccccg acattctcaa aggcattcat ctttccatgg
     2581 ctggccgcta cctgaagctg ctgcgggcga ttgatgtgca tggccaggtg gcgatcaccg
     2641 gcggtctctc cgcggacacc ggcctggcgc aggctttgca ggaaagcgca cgcaaagacg
     2701 gcctcgacgt ggaaatccgc acccacccca acgccatcta cgccggcgcg ctgggggcgg
     2761 ctttgtgggg agcctatcgt catcgtatgc tggctggtca ggtggaggta tcgcaatgac
     2821 cagcgccaaa gaattggcgg gcaagaccgc cgtggtcacc ggcggttcgg gaggaatcgg
     2881 aagcgccatc gttcgcacct tgcaacaagc tggagccgtg gcaacttctc tcgatctctc
     2941 gccaccgccc gataatgaca ttccctggct gcattgcgat gtgcgcagcg acgattccgt
     3001 gggctccgcc gtgcaacagt tctttcagca gaatgggcgg ctcgatctgg tgattcacgc
     3061 cgccggaatc tcgcgtgaag cggtcgtgtg gaagcttacg gtcgaagatt gggacgcgac
     3121 ccaagcggtc aatttgcgtg gcgctttctt gctgttgcgg cacgctattc ccctgatgcg
     3181 ccagggacag ggtgggcgcg tggtcttgat cggctccatc aacggatcgc gcggcaagtt
     3241 tggtacctcg gcctactcag ccagtaaagc gggactgctt ggcttagcca agagcgtcgc
     3301 ccgcgaaacc ggccgctttg gaattctgct gaacgtgatc gagccgggct gggtacgcac
     3361 accactcaca gaagcattgc cggcagccat ccgccaggcc gcggaagcgg aaaccctgtt
     3421 gggcagtc
//
LOCUS       DATMGX010000930         2486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_339729, whole genome shotgun sequence.
ACCESSION   DATMGX010000930 DATMGX010000000
VERSION     DATMGX010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339729"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VNR65_15195"
     CDS             complement(<1..1115)
                     /locus_tag="VNR65_15195"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HWJ20064.1"
                     /translation="MFAFNNSYARLPERFYARLAPIQVASPRLVRLNEKLALHLNLDP
                     AALSMPDGVAVLAGNRIPEHADPVSMAYAGHQFGMFVPQLGDGRAILLGEVIDRDGVR
                     RDIQLKGSGRTPFSRSGDGRAALGPVLREYIVSEAMAALGIPTTRSLAAVTTGETVLR
                     DRPLPGAVLTRVAQSHVRIGTFQFFAARGDVEATRHLADYVIARHYPEAAHEENPTLA
                     LLNLVMARQADLIAKWVLIGFIHGVMYTDNMSIVGETIDYGPCAFMDTYHPETVFSSI
                     DHFGRYAYGNQPRIAQWNLTRFAETLLPLLSEDTNAAVEVAQTRLAGFMPLFEQAFAA
                     GLRHKLGLLRPEAGDTLLAKDLLDRMAENGVDFTLTFR"
     gene            complement(1166..1324)
                     /locus_tag="VNR65_15200"
     CDS             complement(1166..1324)
                     /locus_tag="VNR65_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20065.1"
                     /translation="MLLLLLGYASLIVFDVRAAWRLEAPPYFARLRSVQMLIPIASLG
                     FMLILSRG"
     gene            complement(1726..2124)
                     /locus_tag="VNR65_15205"
     CDS             complement(1726..2124)
                     /locus_tag="VNR65_15205"
                     /inference="COORDINATES: protein motif:HMM:NF006040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HWJ20066.1"
                     /translation="MGLLSDLFTWWHGATLSTKLYTSARGQFVGEDETGNRYYQDASG
                     KGTAGRPRRWVIYEGLAEASKVPPEWFGWLHYTVDTPPTEEAYHPRPWQKPHVENMTG
                     TPLAYRPPGSLVGEMRRPKADGDYQPWKAE"
BASE COUNT          514 a          733 c          775 g          464 t
ORIGIN      
        1 cggaacgtca gcgtgaagtc gacgccgttc tcagccatcc ggtcgagcaa gtctttggca
       61 aggagcgtgt ctccggcttc gggtcgaagc agtccgagct tgtgccgaag accggccgcg
      121 aaagcctgtt cgaagagagg catgaagcca gcgagtctcg tctgagctac ctcaacagcg
      181 gcgttcgtgt cttctgagag aagcggcagc agcgtctctg cgaagcgggt caggttccac
      241 tgcgcgatgc gcggctggtt gccataggcg tagcggccga aatggtcgat cgaactgaag
      301 acggtttcag ggtgataagt gtccatgaag gcgcaagggc cgtagtcgat ggtctcgccc
      361 acgatcgaca tgttgtcggt gtacatcacg ccatgaatga aaccgatgag cacccacttt
      421 gcgatgaggt ctgcctggcg cgccatcaca aggttcaaaa gcgcgagggt ggggttctcc
      481 tcgtgagcgg cttcagggta atggcgggca atgacgtaat ccgcgagatg gcgggtagcc
      541 tccacgtcgc cgcgcgccgc aaagaactgg aaggtgccga tgcggacgtg gctctgggcc
      601 acccttgtga gcaccgcgcc cggaagcggc ctgtcgcgca ggaccgtctc tccggttgtg
      661 accgccgcaa gcgatcgcgt ggttggaatc ccgagcgccg ccatggcctc gctcacgata
      721 tattcgcgaa gcacgggccc aagcgccgcg cgtccatcgc cgctgcggga aaagggcgtc
      781 ctgcccgaac cttttaactg aatgtctctg cggacgccgt cgcgatcgat aacctcgccc
      841 agcaagatgg cgcggccgtc tccgagctga ggaacaaaca taccgaactg gtggcctgca
      901 taggccatcg agacgggatc ggcgtgctcg ggaattcggt tgcccgcgag aacggcaacg
      961 ccgtcaggca tggaaagggc ggcggggtct aggttgagat gcagggcaag cttctcgttc
     1021 aaccggacaa gccgaggcga tgcgacttga atcggagcaa gccgcgcata gaagcgctcc
     1081 gggagccggg cgtagctgtt gttaaaggca aacaaaggat gattcctgct tctgtgttag
     1141 agcaggcgcg gcgttgcgct acccgctagc ctcttgacag gataagcata aatccgaggc
     1201 tggcaatcgg gattagcatc tggaccgatc gcaagcgcgc gaaataggga ggcgcttcaa
     1261 ggcgccacgc ggccctgaca tcgaaaacga tcaggctcgc atagccgaga aggagaagca
     1321 gcatcgcggg ctccttcgag ccaagagccc agagcacgag ggaaccaagt ccggcggtga
     1381 aaataccaca tcgaggccgc gatctggccg gaagttacgc cattcgggct ccggaagctg
     1441 aggccccggc ggactcccgc aaggaaaaga aggatggcag ctccccaaag cacgatcaga
     1501 tcgaagcaga gctgaacgat tgcagcgttg ccgatgaatg cggcaccggc cgccccggag
     1561 atgaagggga tcgttgcgcc ataaccaagg ctcaagctga gaactggcgc attcagcggt
     1621 tccgctacgg tgaaagcgac ttgattgcta ttccaggcga ttgtcatgct gcgacaaccc
     1681 gccgcagcag cgcttgttcc tgggatcacg ttacggcaac gctgcttact cagccttcca
     1741 aggctgataa tcgccgtctg ccttcggccg gcgcatctcg ccaacaaggc ttcccggcgg
     1801 tcggtaggcc agcggcgtcc ctgtcatatt ctcgacatgc ggcttttgcc acggcctcgg
     1861 atggtaggct tcctccgtgg gaggcgtatc gaccgtgtaa tgcagccagc caaaccactc
     1921 cggtggaacc ttgctggcct cggccaagcc ctcgtagatc acccagcggc gtggccgtcc
     1981 ggccgtgccc tttccgctcg cgtcctggta atagcgattg ccggtttcat cctctccgac
     2041 aaattggccg cgcgcgctcg tgtaaagctt cgtcgaaagc gtcgcgccgt gccaccacgt
     2101 gaagagatcg ctgagtaagc ccattcccgt ccccaaattt tgttaggttt tacgctaatc
     2161 cgagccaagc gtccaccaac gcgacaggga ccggcagaga attcgccctc cacgaggtgt
     2221 ctgcgcagct tgtacattcc agaaagcctg catagtccag gcttgtgaac catgtggaga
     2281 gggcatgcgt gcgactgttt ctgaagctct gatcaccaat tcggatgctt ttcatgactg
     2341 ggcggccaag tgccgatcct ccttggggcc ccgggtcacc gtagcattgg atccagggat
     2401 gctaggctgg cagatgcctt gaaggacgag tgcgtccgcc tgcgcaaacg ctacaagcat
     2461 tctccattcc ttgttctatc tgcgct
//
LOCUS       DATMGX010000931         5091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_52594, whole genome shotgun sequence.
ACCESSION   DATMGX010000931 DATMGX010000000
VERSION     DATMGX010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52594"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VNR65_15210"
     CDS             <1..452
                     /locus_tag="VNR65_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20067.1"
                     /translation="LERLSPGRLHDLAPEGSEIWVDGGHNPAAGEVVARALADLEERV
                     SSPIHLIVGMLSTKDASGFLRHFQGLTELAATVEIPGHTAAYKADELATIANREGIAA
                     DPASSLEAAFASSRKLAQGPVRIMVTGSLYLAGQVLEAHENGFGRSC"
     gene            765..1838
                     /locus_tag="VNR65_15215"
     CDS             765..1838
                     /locus_tag="VNR65_15215"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HWJ20068.1"
                     /translation="MVLDSGRPLSGRRLGSWDMSANPVLIEVTRGAAVESRHRGAFVV
                     VDASGKIVQSAGDPHMPIYPRSAVKPLQAIPLIETGAADRFGLTTKELALACASHKGE
                     PAHVEAVANWLARIGLSPAALECGIQAPRTPDAARKAILAGAPLNAAYHNCSGKHTGF
                     LTTCVACGDNPSGYIEPGHPAQTRVARALAEMTGCDVSAVPVGRDGCGIPTYHMPLHG
                     LATGMARLADPSKLPEARAQAAKRLLNAMAAEPFYVNGTGGFTTEVMTAVPAVRVKGG
                     AEGVYAAALPELGLGMALKIDDGAMRAAECVAAHILRGLQCFGAAEKQRLQLFLNPVI
                     KSDAGRDAGTIRPTAALAFPVLP"
     gene            complement(1846..2247)
                     /locus_tag="VNR65_15220"
     CDS             complement(1846..2247)
                     /locus_tag="VNR65_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20069.1"
                     /translation="MIKTFLPALRRTSAAIILITGTASTVAEDAKGHVPKKAGQCVRT
                     EISELGSRLEGSPDSGSAIAYTNGVTGVSYDIITAIRRSRVGDPITLCLVSVPRNCPP
                     GDDRGKVYSAVNGRTQERWSLPDSQHSCGGA"
     gene            2368..3351
                     /locus_tag="VNR65_15225"
     CDS             2368..3351
                     /locus_tag="VNR65_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HWJ20070.1"
                     /translation="MTDVGSHTPQKLPRMPNISGAAKLLIPVLVLLFIVSECFFIVQP
                     TEMAGVRRLGTILSKEPYQPGFHFKAPFIDKVDKIQVSIDTLRIDNLRVYTVDNQSVQ
                     ISVGVTYRIPRDAVMKLLYEVGRSGNLDINHNIEQIIADRALRLFAKRNTVKISEERE
                     QIAGDVRVSVSQSVRELFGIEIFDLQIPAIRYSDTFVASVEAAVKAKNDAIAAENTVN
                     RIRYEGEQKVVTARAEAEAQIARAQAQKQSQILAAEGEARSIELRTAAEALGIRVRGD
                     ALKENARVIELTLAQRWSGQPPQTILGEHGAVPFFNLGNNGMAPQPAQPSR"
     gene            3436..4743
                     /gene="ccrA"
                     /locus_tag="VNR65_15230"
     CDS             3436..4743
                     /gene="ccrA"
                     /locus_tag="VNR65_15230"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HWJ20071.1"
                     /translation="MSEVIAFKQSTTSNSGGGLKDLYEVGEIPPLGHVPEKMYGWSIR
                     KERHGPPDEAMQIEVLPTWKLDSHDVLIFVMAAGVNYNGVWAALGIPISTLDGHKNPY
                     HIAGSDASGIVWAVGAKVKRWKVGDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGYE
                     TPDGSFAQFARVQDRQLMARPQHLTWEESACYTLTLATAYRMLFGHRPHVLRPGHNVL
                     VWGASGGLGSFAVQLCATSGANAIGVVSEDDKRDFVMSLGAKGIINRKDFKCWGQMPK
                     VGTPEYGEWFKEVRKFGAAIWQHTGKGNNVDSVFEHPGEATIPVSVFVVKRGGMVVIC
                     AGTTGYNLTLDARYLWMHQKRLQGSHFANLLQASQANKLVIERRIDPCMSEVFPWTDI
                     PKAHMRMWKNEHRPGNMAVLVSAPHTGLRTFEDAIEASKARFG"
     gene            4796..>5091
                     /locus_tag="VNR65_15235"
     CDS             4796..>5091
                     /locus_tag="VNR65_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20072.1"
                     /translation="MTRAEDLLKALNGLRNAADTELSGEAHDVAMQHIKGLFRLADFD
                     GEDTGEGANQATIPFAQAIAEVRTQAEGEMSGNAFYLASKSLDDLANLSPSPASA"
BASE COUNT         1050 a         1509 c         1549 g          983 t
ORIGIN      
        1 ggcttgagcg cctgtcaccc ggtaggctcc atgatctcgc gcccgagggc tcggagatct
       61 gggttgatgg cggacataat ccagccgccg gcgaggtcgt agcccgggcg ctggccgacc
      121 tcgaggagcg cgtttcgagc ccgattcatc tgattgtcgg catgctgtcg acgaaggatg
      181 cgagcggctt tttgcggcat ttccaggggc tgacggaact cgccgcgacg gtggaaattc
      241 cgggtcatac agcggcctat aaggccgacg aacttgcgac catcgcgaac cgcgaaggca
      301 tcgctgccga tccggcttcc agcctcgaag cggcgttcgc atcaagccgc aagctcgcac
      361 agggccccgt gcgaatcatg gtgaccggct cgctctatct tgccgggcag gtgctggagg
      421 ctcacgaaaa cggttttggc cgcagctgct agattaccgg cctgaaccgt taaagcgtgc
      481 cgaaattatt cggaatcccc cttttgtcat ggccgccctc ctccaggata acaaatttca
      541 ccttttggat tttaaaactg gatggccggg cttgacccgg ccatccagaa aacacaaggt
      601 attcaagcat agctggatgg ccgggtcagg cccggccatg acaggtgacc gaaaattcgc
      661 tagtgttttc aaggcttctt ctgcgggcga aaacattcgg gtaatcaagt cccgggaaac
      721 ccaacggcgt tgtccgggat cattaatccg cgcttgcgtt tggaatggtc ctggatagcg
      781 gcaggccgct ttcgggacga cggcttgggt cgtgggacat gagtgcaaat ccggttctca
      841 tagaggttac acggggcgcg gcggtggaga gccgccaccg cggtgccttt gtggttgtcg
      901 atgcatccgg gaaaatcgtg caaagcgcgg gcgacccgca catgccgatt tatccgcgct
      961 cggctgtgaa gccgctccaa gcgatcccat tgatcgagac cggagctgcc gatcggttcg
     1021 gcctaacgac caaggaactg gcgcttgcct gcgcatcgca caaaggcgag cccgcgcatg
     1081 tcgaagcggt cgcgaactgg ctcgcgcgca tcggcctcag tcccgccgcg ctcgaatgcg
     1141 gaatccaggc gccgcgcacg cccgacgccg caagaaaggc gatcctcgcg ggcgctcctc
     1201 tcaatgccgc ctaccacaat tgctccggca aacacacagg gtttctcact acttgcgtgg
     1261 cctgtggcga caacccgtcc ggctacatcg agcctggtca cccggcgcaa acacgggttg
     1321 cgcgagcgct cgcggagatg accggctgtg atgtgagcgc cgttcctgtt ggccgtgatg
     1381 gatgcggcat ccccacctat cacatgcccc tgcatggcct cgcgaccggc atggcgcggc
     1441 ttgccgaccc atcgaaactg cctgaagcga gagcacaagc tgcgaagcgg cttctgaatg
     1501 ccatggccgc cgaacccttc tatgtaaacg gcaccggcgg gttcaccacg gaggtcatga
     1561 cggcagttcc cgcggttcgt gtgaaggggg gcgccgaggg cgtctatgcc gctgctttac
     1621 cggagttggg tctcggcatg gcgctgaaaa tcgacgatgg cgccatgcgc gccgcggagt
     1681 gcgtggccgc ccacatcctt cgcgggcttc aatgcttcgg ggcggctgaa aagcagcggc
     1741 tgcagctctt tctcaacccg gtcattaaat ccgatgcggg ccgcgacgca ggaacgatcc
     1801 gccccacggc tgcgcttgcc ttcccggtcc tgccttgaaa cgcgtctacg cgccgccgca
     1861 actatgctgg ctgtcgggca gagaccagcg ctcctgggtc cggccattga ccgcggaata
     1921 gaccttgcct ctgtcgtcgc ccggcggaca gttgcgcggg acggaaacaa ggcaaagggt
     1981 gatcggatcg cccacacgtg agcgccggat ggctgtgatg atgtcgtagc tcacgccggt
     2041 aacgccgttt gtgtaggcaa tggcggagcc gctatcgggg cttccttcca gtctcgaacc
     2101 gagttcgctg atttcagttc gaacgcattg accggccttt ttggggacat gtccctttgc
     2161 gtcttccgca acggtggaag ctgtcccggt aatcagaatg atggcggcgg atgttcttcg
     2221 cagcgccgga agaaaagtct ttatcaaaca aacctcctct taatgcttat ggaatctgcg
     2281 ccctgtgttt gtcacagaag cgccaccttg cgaacacgaa aaatacgagc ttcgcgatcg
     2341 tacatagcgc agccaatgga ggacgccatg acggatgtgg gctcacacac accccagaaa
     2401 ctgccccgga tgccgaacat atcaggagcg gctaagctct tgatcccggt actcgtgctg
     2461 cttttcatcg tcagcgagtg cttctttatc gttcaaccga ccgaaatggc cggcgtcagg
     2521 cgtcttggaa ccattctcag caaggaaccg tatcagccgg gcttccactt caaggcgccc
     2581 ttcatcgaca aggtggacaa gatccaggtc tccatcgaca ccttgcgcat cgataatctg
     2641 cgcgtgtaca cagtcgacaa tcagtccgtg cagatcagcg tcggtgtgac ctaccgcata
     2701 ccgcgcgacg ccgtcatgaa acttctctat gaggtgggac ggtccggaaa cctcgacatc
     2761 aaccacaaca tcgagcagat catcgccgac cgagcgctgc gcctttttgc gaagcgcaac
     2821 accgtcaaga tttccgagga acgcgagcag atcgcgggcg atgtccgcgt ttccgtcagt
     2881 cagtccgtgc gggaactttt tggcatcgag atctttgacc tacagatccc ggcgatcaga
     2941 tattcagata cattcgttgc atctgtggag gcagcggtga aagccaagaa tgacgcaatc
     3001 gccgccgaaa acacggtgaa ccgtattcgc tatgaaggtg agcagaaggt tgtcacagcc
     3061 cgcgccgaag ccgaagcgca gattgcccgc gctcaggcgc agaaacagtc tcaaatcctt
     3121 gccgcggagg gcgaagctcg cagtatcgaa cttcgcacgg cggcggaagc gctcggcatc
     3181 cgcgttcgcg gcgacgctct gaaggaaaac gctcgcgtga ttgagctgac acttgcccag
     3241 cgttggagcg gccagccgcc gcaaacaatt ctcggtgaac acggagctgt gccgtttttc
     3301 aatctcggga acaatggcat ggcaccacaa ccggcgcagc cttcgcgtta aggctatgtt
     3361 gcgctccact tgaaaaggcg tcatgctgca ctgcaaacaa gaccaacaag tcgcgctacg
     3421 cgcgaggagc gttctatgag tgaggtcatt gcgttcaagc aatccacaac ctcgaattca
     3481 ggaggcggct tgaaggatct ttatgaagtc ggagagattc cgcctctcgg tcacgttccg
     3541 gaaaagatgt acggctggtc catccgcaag gagcgccacg gcccgcccga tgaggcgatg
     3601 cagatcgagg tgttgccgac ctggaagctc gacagccatg acgtgttgat cttcgtcatg
     3661 gcggcaggcg tcaattataa cggtgtttgg gctgctctgg gcattcccat ttccacgctc
     3721 gatggacaca aaaatcccta tcacattgca gggtcggatg cctccggtat cgtgtgggcg
     3781 gtcggcgcga aggtgaagcg ctggaaagtc ggcgacgaag tcgttgtgca ctgtaaccag
     3841 gacgatggcg acgacgagga gtgcaacggc ggcgatccca tgttttcgcc ctcccagcgc
     3901 atctgggggt acgagacgcc ggacggctct tttgctcagt tcgcccgcgt gcaggaccgc
     3961 cagctgatgg cgcgcccgca gcatctgacc tgggaagaaa gcgcgtgcta tacgctcacg
     4021 ctggccacgg cttaccgcat gctgttcggg catcgcccgc acgttctgcg gcccggccat
     4081 aatgtgctgg tttggggcgc gagcggcggt ctcggctcgt ttgcggtgca gctttgcgcg
     4141 acttcgggcg cgaacgcgat cggcgtcgtc tccgaagacg acaagcggga tttcgtcatg
     4201 tcgcttggcg cgaagggaat catcaaccgc aaggacttca agtgctgggg gcagatgccc
     4261 aaagtcggca cgcccgaata tggggaatgg ttcaaggaag tccgcaagtt cggggctgcg
     4321 atctggcagc acaccggcaa agggaacaac gtggacagcg tcttcgagca tccgggcgaa
     4381 gcgaccatcc ctgtttcagt gttcgtggtg aagcgcggcg gcatggtcgt catttgcgcc
     4441 ggtaccacag gctataacct cacactggac gcccgatatc tctggatgca ccagaaacgt
     4501 ctccagggat cccacttcgc caacctgttg caggcaagcc aagccaacaa gctcgtcatc
     4561 gagcgtcgaa ttgacccgtg catgagcgaa gttttcccct ggaccgacat cccgaaagcg
     4621 catatgcgca tgtggaagaa cgagcatcgg ccgggcaata tggcggttct cgtcagcgcg
     4681 ccgcatactg gcttgcgtac attcgaggat gcaatcgagg catccaaagc gcggttcggt
     4741 tgacgacccg gcttcgttct gactccgcgc aacgcgaaag agatacggag cccacatgac
     4801 gcgtgcagag gacttattga aggcgctgaa cggcctcagg aatgcggctg acacagagct
     4861 gagcggcgaa gcccacgacg tggcgatgca gcatatcaag ggtttgttcc gccttgccga
     4921 ttttgacggc gaagacacgg gcgagggggc aaaccaagcg accataccgt ttgcgcaggc
     4981 gattgccgag gttcgaacgc aggccgaagg ggaaatgtcg ggcaatgcgt tctatctcgc
     5041 atccaagagc ctcgacgatc tggcaaatct gtcaccctct ccggcaagcg c
//
LOCUS       DATMGX010000932         2747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_220684, whole genome shotgun sequence.
ACCESSION   DATMGX010000932 DATMGX010000000
VERSION     DATMGX010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220684"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VNR65_15240"
     CDS             complement(<1..530)
                     /locus_tag="VNR65_15240"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20073.1"
                     /translation="MTASLRVVNPGMAATLQDSGREGYQRFGVPVSGALDRVSLAIAN
                     VLVGNAPHEAAVEVLAAGLTVMVCSDSVVLAVAGMADPSIVQTAQADIRVTPFQSITA
                     RRGDIVRFPPPKGGAVFYVAAAGGFDIPSAFGSRSTYRRATLGGFHGRALQAGDDLPL
                     RLPDASAREALSLDVSL"
     gene            complement(527..1249)
                     /gene="pxpB"
                     /locus_tag="VNR65_15245"
     CDS             complement(527..1249)
                     /gene="pxpB"
                     /locus_tag="VNR65_15245"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HWJ20074.1"
                     /translation="MRLCPTFSDCAESALLVDFGLHYDRQLSLSILQVSTILQASMLP
                     GLKESIPALSSLTVLYDPLELSRERIAREIRHLCEVTGAVPDCARTWEIPVVYGGEGG
                     PDLEEVALSAGITEEEAVALHAGRDYFVYMLGFLPGFAYLGDLPKELETPRRATPRAR
                     VPAGSVAIASGMTAIYPLESPGGWHVIGYTPVNPWDLKRRKEPLFQAGDRVCFKKVEA
                     READEIQRLAAEGWTPACREGA"
     gene            1850..2401
                     /locus_tag="VNR65_15250"
     CDS             1850..2401
                     /locus_tag="VNR65_15250"
                     /inference="COORDINATES: protein motif:HMM:NF017277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HWJ20075.1"
                     /translation="MKQANKVKDLGSKRKSEPRWLDKTEIQGNSYKTLDSSAQGRSRS
                     PQMIEEKFENNEIVEFTTQIVSAYVSNNSVVAGDLPSLIGDVHSALNRASSNLVQAPK
                     EELKPAVAVKKSLTPDYIICLEDGKKFKSLKRHLRTHYNMSPEEYREKWGLEPNYPMV
                     APKYAEARSNLAKKMGLGQRKGR"
BASE COUNT          666 a          737 c          787 g          557 t
ORIGIN      
        1 aggctcacat cgagcgatag agcttcgcga gcggacgcat cggggaggcg gaggggcaga
       61 tcgtccccag cctgtagcgc ccgtccgtgg aagccgccga gcgtggcgcg tcgataggtg
      121 gatctactgc caaaagcgga aggaatatcg aagccgcccg ccgccgcaac atagaagaca
      181 gcgcctccct tcgggggagg gaaacgcaca atgtctccgc gtctcgcggt gatgctctga
      241 aacggggtga ctcggatatc cgcttgcgct gtttgaacaa tagagggatc cgccatccca
      301 gctacggcaa gaacgacgct atccgagcaa accattactg tcagcccggc cgccagaacc
      361 tcaaccgcgg cttcatgggg cgcattgcct accaggacat tggcgatagc caggctgaca
      421 cgatcaagcg cgccggaaac tggaacgccg aagcgttgat agccttcccg accgctatcc
      481 tgaagcgtcg cagccatgcc aggattgacg acccggagcg aggcggtcac gccccctccc
      541 tgcacgcggg cgtccagccc tccgccgcca gccgctggat ttcgtcggct tccctcgctt
      601 cgaccttttt gaagcaaacc cggtcaccgg cttggaagag tggctccttt cgccgcttca
      661 gatcccaagg gttgacgggc gtatatccga tgacgtgcca accgccagga ctttcaagcg
      721 ggtagatggc ggtcatgccc gaggctattg cgaccgatcc cgctggcacg cgggcgcgtg
      781 gcgtggcgcg tcgcggcgtt tccagctctt ttggcagatc gccgagataa gcaaagcccg
      841 gaagaaaacc cagcatatag acaaagtagt cccgcccggc atgaagtgcg accgcctctt
      901 cttcggtaat cccggcgctt agggcgacct cttccaggtc cgggccacct tcgccgccat
      961 aaacaacggg aatttcccaa gtccgtgcac agtccggtac tgcgccggtg acctcacaca
     1021 gatgccgaat ttcgcgggcg atcctctctc tggaaagctc caacggatcg tacagaaccg
     1081 tcagggatga aagggccgga atgctttcct tgagccccgg aagcattgag gcctgaagaa
     1141 tagtgctgac ttgaagaatc gacaggctga gctgccgatc ataatggagc ccaaagtcaa
     1201 cgagcagggc actttcggcg caatctgaga aggtgggaca cagccgcatc tgcaaatttc
     1261 cattttgagc tacccagcct aagaaaatgg ggagctgata ggccgattcc gaggagttcg
     1321 ggcaacacgg cggcaaaaca tgtatccaca ttgtgtggaa agcccgagat aagctttgcc
     1381 tctgacagta tcgcttggga taggtttgtg gcggccgtgg ccgggagcct tagctaagag
     1441 tttaccttgt tggaggaggc aatctcttct acgtcacgac gatcagccac agttgcacgc
     1501 aatcgcatca ggttaatgag tgacctgttg catatggatg aagcaaagat ctaaatatag
     1561 ctccctggta tggcgcatac ggcatgtagc ataggctcca taaaggcaac agtgctgcgc
     1621 agcaaggaag tgcaacggga ccgcttatga attcgcgtct cggctagctg cggcattttg
     1681 gacaaaccaa gcagagttta ccaagccaca aaaaagagca tgtttttttg agagacagct
     1741 atgcgcgtcg aactaaagta acgtgtgtta ttgtcaatag aattcttgag aagttgcgtc
     1801 tttgtgctat acaggatgta ttccgtgaaa atgcgaaagg cagacgattg tgaagcaagc
     1861 aaataaggtc aaggatctcg gttcaaagcg taagtctgaa ccgcgctggc ttgacaaaac
     1921 agagatccaa ggtaattcct acaaaacatt ggacagttca gctcaaggcc gttccaggag
     1981 cccgcaaatg attgaggaaa aattcgaaaa taacgagatt gtggaattca cgacacagat
     2041 cgtttctgcc tatgttagca ataactctgt agtggctggt gacttgccga gcttgattgg
     2101 ggacgtccac tccgcactta accgtgcgtc gtcaaatctg gttcaggcac cgaaggaaga
     2161 gttgaagcca gcagtagcgg taaagaaatc tctgacaccg gattacatca tttgtttgga
     2221 agatggcaag aagtttaagt cactgaagcg ccatctgcgc acgcactaca acatgtcccc
     2281 cgaagaatat cgtgaaaagt ggggcttgga gccaaattac ccgatggtgg cgccaaagta
     2341 cgccgaagct cgctcgaatc ttgctaagaa gatgggcctt ggacagcgca aggggcggta
     2401 acccggccct ttcctctgtt tcaagtaaag gctagacccg taattatccg gctcacgcgg
     2461 atagcgagac agtttttctg cggcgtcccg gggggcgcag cggaatagct tgcactgcaa
     2521 catgcaaatc accgatagtt cccgagtcat ggtgatcggt atgccgaagg cggatcggca
     2581 gacgaacatc ggaggggtga atgacttcgg aactcgcttt ccgcgcgcag gttatattgc
     2641 gcgagcaggg ggagctatac gagtattggc gagagtgcgc gcagtctcgg ccgatcccct
     2701 ctcgttttga cattgacccc gttgccattc cgcatcttct cccgggg
//
LOCUS       DATMGX010000933         2771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_69360, whole genome shotgun sequence.
ACCESSION   DATMGX010000933 DATMGX010000000
VERSION     DATMGX010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69360"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VNR65_15255"
     CDS             complement(<1..1024)
                     /locus_tag="VNR65_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20076.1"
                     /translation="MDSSSGGDAKSAARAPRTRSRRSRLLFEKRIALLAVLAALPGIA
                     FGTILIWTHPWTREVKIALTALEFFLWLVLTLALLDQIVRPLQTLTNVVGALREEDYS
                     FRARGAVANDALGELALEINALADILTEQRIQTIEATALLRRVVDEIDAPLFTFDPDH
                     ILRLVNAAGERLLQQPSARLLGKTAAELELNACFEARNETLVALPYSTPNARWMVRQS
                     SFRQNGVPHKLIVLSDVSRALREEERSAWQRLIRVLGHELNNSLAPIISIAGSLASRL
                     PLPQLPEEQNADFQRGLNIIESRTASLHRFLQSYRRLAQMPSPTLQPVELRSLLERVA
                     VLETRLN"
     gene            complement(1014..2405)
                     /locus_tag="VNR65_15260"
     CDS             complement(1014..2405)
                     /locus_tag="VNR65_15260"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWJ20077.1"
                     /translation="MVQDTATGTRDRQKGGRILAADDQPHILEAIELLVRPQGFEVDT
                     AKSPAAVREALASDFYDAVLIDLNYTRDTTSGQEGLDLLSEIVAFDSSIPVIVMTAWG
                     NVGLAVEAMRRGARDFIQKPWENERLLAILRTQVELHRALQRTQRLEAEAKLLRAEGR
                     PDFIAAAPSMQPVLQTIARIGPSDANVLVTGEHGTGKEIVAQTLHALSARASMPLVAV
                     NTGALPEGTFESELFGHVKGAFTDARMDRIGRFELADNGTIFLDEIGNVPMRQQAKLL
                     RVLETGEIERVGSSRTKRINVRVISATNIDLQTAIAAGQFREDLLFRLNTVEIHIPAL
                     RERREDIAALAAHFLSRYAARYRAPVSGFAAPALQTMMQYAWPGNVRELEHTLERAVL
                     MCHTGEIQVSDLGLNVQRPQGQNLEELSIEEVEGILVRKALQRFHGNVSQAAEALGLS
                     RGALYRRMERYGL"
     gene            complement(2450..>2771)
                     /locus_tag="VNR65_15265"
     CDS             complement(2450..>2771)
                     /locus_tag="VNR65_15265"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWJ20078.1"
                     /translation="LKKQIRIEVRNAQYALEQGRARVRAAKKARDLADKTFNIMQKEQ
                     ALGAGSSFQTLTAQRDLAVAELDLVNATTAYQKSKLELERATGTTLEDNGIQIQDAVN
                     GTAP"
BASE COUNT          536 a          815 c          838 g          582 t
ORIGIN      
        1 cgttaaggcg agtctccagg accgccacgc gctccagcaa ggagcgtagc tcaactggtt
       61 ggagggtggg cgaaggcatc tgcgccagcc tgcgataaga ctgaagaaat cgatgcagcg
      121 atgcagtgcg tgattcgatg atgttgagtc cgcgttgaaa gtccgcgttc tgttcttccg
      181 gcaattgagg caggggcaga cgcgaagcca ggctccccgc gatcgaaata ataggcgcca
      241 gtgagttatt gagttcatgg ccgagaacgc ggatcaacct ctgccatgcg ctgcgctctt
      301 cttcgcgcag ggcacggctc acgtcggaga gcacgatcag tttgtggggc acgccgtttt
      361 ggcgaaaaga actctggcgc accatccagc gtgcgttagg agtggagtac gggagcgcga
      421 ccaaggtttc gtttctggct tcgaagcagg cattgagttc gagttccgct gcggtcttcc
      481 ccagcagccg agccgatggc tgttgcaata gccgttcgcc tgccgcgttt accaggcgca
      541 ggatgtgatc gggatcgaag gtaaacagcg gggcatcgat ttcatcgacc acccgtcgca
      601 aaagcgcggt ggcttcaatc gtctggatgc gctgctcggt caggatgtcg gcaagggcat
      661 tgatttcgag cgccaactcg cccagagcat cgttggctac cgccccacgc gcgcgaaagg
      721 aatagtcttc ttcacgcagc gcgcccacga cgttggtcag agtttgcagc ggccgtacga
      781 tctggtcgag cagcgctaac gtcagcacca gccaaaggaa gaactcaagc gccgtcaacg
      841 cgatcttcac ttcgcgcgtc catggatgag tccagataag gatggtgccg aaggcgatac
      901 ctggaagggc cgccagaaca gctaacagag caatacgttt ttcgaagagt agcctcgatc
      961 ttcgagatcg agtgcgaggg gctcgggccg cggacttggc gtcgccgcct gaactagagt
     1021 ccataccgtt ccatccggcg gtagagagcg ccacggctca agcccagggc ctcagccgcc
     1081 tgactcacgt tgccgtggaa ccgctgcagc gctttgcgaa ccaggatgcc ctcgacctct
     1141 tcgatgctga gttcttccag attttgcccc tgcgggcgtt gcacattcag tccgagatcc
     1201 gagacttgaa tctcgccggt gtggcacatc agcaccgcac gttccagggt gtgctccagt
     1261 tcgcggacgt tccccggcca ggcatactgc atcatcgtct gaagagcggg ggcggcaaaa
     1321 ccgctcaccg gcgcgcgata gcgcgccgca taacgcgaca ggaaatgtgc cgccagcgct
     1381 gcaatatcct cgcggcgttc gcgcagggca gggatgtgga tctcaaccgt attcagccgg
     1441 aaaagaaggt cttctctaaa ctggcccgcg gcaatagcgg tttgcagatc gatgttggtc
     1501 gcggaaatga cgcgcacgtt gatccgcttg gtccgggatg agccgacccg ctcgatctcg
     1561 cccgtttcca gcacgcgcag caacttggcc tgctgccgca tgggaacgtt tccaatttca
     1621 tcgagaaaga tggtgccgtt gtcggccaat tcgaaacgtc cgatgcggtc catacgcgcg
     1681 tccgtaaacg cgcctttcac gtggccgaat aattcgcttt cgaaggtgcc ctctgggagc
     1741 gcgccggtgt ttacggctac cagcggcatc gaagcccgcg ccgataacgc atgaagagtc
     1801 tgggccacta tctctttgcc cgtgccatgt tcgccagtga ccagcacatt ggcgtcggag
     1861 gggccgatgc gggcaatggt ttgcaatacg ggctgcatgg aaggagcagc ggcgatgaaa
     1921 tcgggacgtc cttcggcgcg caacagtttc gcttccgctt ccaggcgctg ggtacgttgc
     1981 agagctcgat gcagttcgac ctgcgtacgc agaatggcca acaagcgttc gttctcccac
     2041 ggcttctgaa tgaagtcgcg ggcaccgcgt cgcatggcct cgactgccag ccccacgttg
     2101 ccccaggccg tcatcacgat aaccgggatg ctgctatcga aggccacaat ctcggagagc
     2161 agatccagcc cttcctgacc cgaggtagta tcgcgggtgt agttcaaatc aatcagtaca
     2221 gcatcgtaga agtcgctggc gagagcttcc cgcaccgctg ctggagactt cgcggtatcg
     2281 acttcaaagc cctgcgggcg aactagcagt tcgatggctt cgagaatgtg cggttgatcg
     2341 tcagccgcaa gaatgcggcc tcctttctgc cggtcacgtg tcccggttgc ggtatcttgt
     2401 accacccacg agcttccttg gctttgcgtc ttcggcaaca tgctctccgt cagggtgcgg
     2461 tgccgttcac cgcatcctgg atctgaatgc cattatcttc cagagtggtt ccggtggcgc
     2521 gttccaactc gagtttggac ttctggtatg ccgtcgtggc attcaccaga tcgagttcgg
     2581 caacggcgag gtcgcgctgg gcggtcaacg tctggaaact cgatcctgcg cccagagctt
     2641 gctccttttg catgatgtta aacgtcttgt ctgccagatc gcgcgctttt ttggctgccc
     2701 gcacccgggc gcggccctgc tcaagcgcat attgcgcgtt gcgtacttcg atgcggattt
     2761 gcttcttcaa t
//
LOCUS       DATMGX010000934         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_57203, whole genome shotgun sequence.
ACCESSION   DATMGX010000934 DATMGX010000000
VERSION     DATMGX010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57203"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1597)
                     /locus_tag="VNR65_15270"
     CDS             complement(221..1597)
                     /locus_tag="VNR65_15270"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HWJ20079.1"
                     /translation="MTDSTATERLLFPEIVAKPVVLEFDQRQGSSDGGAVLLKAAEQR
                     YGLIASMAGCLRDPRQAGKVDHSLRDLFAQRVFSIGCGYADANDSARLAADPMHKMLL
                     DRDPVTGLDMASQPTLSRFENAVGPRQLYSMGTALAESVIGRHAQRLHHRARLVTIDL
                     DPTDDPTHGAQQLSFFNSHYDNACYLPMMGFVTFNNEAEQYLCAAVLRPGNVTAAAGA
                     VGILRRLMALVRNSFPKARIRVRLDGGFANPELLAFLDSEPGVEYVVAMASNAVLNRK
                     AEEAMQYARVLAGLTDQTERVYSEARYAAGSWKRERRVIIKAEVVRTEEKAPKDNPRF
                     VITNMKQTPQWLYEEVYCQRGEIENRIKELHAMEIDRTSCSLFWANQFRVLLTAAAYV
                     LMQELRLSAAGTNCARAQVWTLRERFLKLGARVVASARRMVIHLPQSFPFLTTFRHMA
                     MELGASPG"
     gene            1761..>2565
                     /locus_tag="VNR65_15275"
     CDS             1761..>2565
                     /locus_tag="VNR65_15275"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HWJ20080.1"
                     /translation="MARTVAELPPGARITDYISLGVITRTFPLSAIGPVLAKTGTASI
                     RQRDLPAQVVIYYVIALALYMQSSYREVLRCLLEGVQWLRDPSVGVRVAGKSGISQAR
                     TRLGWEPLRQLHDEWVRPIAVRGTRGAWYRTWRLVSLDGSTFDVADEKVNEDAFSRPG
                     ASRGASAYPQIRFVSLVENGTHVLFGSQMDSYRTGEITLAKAVLPRLGKGMLCLADRN
                     FFGFELWQQARGTGADLLWRMKKNMRMACEKRLPDGSYLSHVYPSERDRA"
BASE COUNT          530 a          739 c          763 g          533 t
ORIGIN      
        1 cactagcctg cattattcgt gaacgccccg cgagcgacga ttttgcgtgg caaaagatcg
       61 cctgggagga tgaaggcagt gtgaagttgc tgtgtgggac atggcggcag gctttgcagg
      121 cccacaccat caaggctgcg acggttttcg ggcgtgtctt tcccttgtgc tcggttgggt
      181 caggcgaaaa tgggaactct tcagaggagc gaagctcgtt ctaccctggc gaggctccca
      241 gttccatcgc catgtgccgg aaggtggtca gaaacgggaa cgattgcggc aagtgtatga
      301 ccatgcgccg cgctgaggca acgaccctcg cgcccaattt caggaagcgt tcccgcagtg
      361 tccagacttg cgcccttgcg cagttggtgc ccgccgcaga caggcgcagt tcctgcatca
      421 gcacataggc cgcggcggtc agcagcacgc ggaactggtt agcccagaag aggctgcaac
      481 tggtgcggtc gatctccatg gcgtgcagtt ccttgatccg gttttcgatt tcgccacgct
      541 ggcagtagac ctcttcgtaa agccattgcg gagtctgctt catattcgtg atcacgaaac
      601 gcgggttatc cttgggcgct ttctcttcgg tgcgcacgac ttcggctttg ataatgacgc
      661 ggcgttctcg cttccaactg cccgccgcat agcgggcttc gctgtaaacg cgctcggtct
      721 gatcggtgag ccccgcaagt acgcgggcgt actgcatggc ctcttccgcc ttgcgattca
      781 acaccgcgtt gctggccatc gccaccacat attcgacgcc gggttcgcta tccaaaaatg
      841 ccagcagttc aggatttgca aagccgccgt cgagacgaac gcggatgcgc gccttcggaa
      901 acgagttacg aaccagcgcc atcaaccggc gcaaaatccc caccgcgcct gccgccgctg
      961 taacattgcc gggccgcaac acagccgcgc acagatactg ctccgcctcg ttgttgaatg
     1021 ttacgaagcc catcatcggc aggtaacacg cgttgtcgta gtggctgttg aaaaaggaca
     1081 actgctgcgc cccatgcgtg ggatcatcgg tcggatcaag atcgatggtg accagccggg
     1141 cgcgatggtg caaccgttgc gcatgacgtc cgattacgct ctcggcaagc gccgttccca
     1201 tactataaag ctgacgcgga ccaacggcat tttcaaagcg cgacagcgtc ggctgcgaag
     1261 ccatatcgag ccccgtaacc ggatcgcggt ccaacagcat cttgtgcatc ggatcggcag
     1321 ccaatcgagc cgagtcgtta gcgtcggcat aaccacagcc gatggagaaa acgcgctgag
     1381 cgaaaagatc ccgcaacgaa tggtcaacct tgcccgcctg ccggggatcg cgcaaacagc
     1441 ctgccatact ggcgatcaat ccgtaacgct gctcagctgc cttcagcagc acggcgccac
     1501 catcggaact gccttgccgc tggtcgaact ccagcaccac cggcttggca acaatctctg
     1561 gaaacaataa acgctctgtc gcggtagaat cggtcaagcg aaagcctctc aatgtgcctt
     1621 aaacactgtc ttgtcaacat gtttgtagca caaaaggctt tcgctacctc aagcttcacg
     1681 aataatgcag gctaagccta atactgttca cttagctaaa tttatggcat tctgtatgag
     1741 acaaggacag gaggccacag gtggcgcgta cggttgcgga actccctccg ggtgcccgga
     1801 ttacggacta catcagtctt ggcgtaataa ccagaacgtt tcctctgtct gctattgggc
     1861 cagtgctggc gaagacggga acggccagta ttcgtcagcg cgatcttccg gcgcaggtgg
     1921 ttatttacta cgtcatcgcc ttggcgcttt acatgcaatc gtcctatcgg gaggtattgc
     1981 gctgtctgct ggaaggagtg cagtggctgc gcgatccatc ggttggcgtt cgggttgcag
     2041 gcaaatcggg catctctcag gcccgcacac ggctgggctg ggagcctctg cggcagttgc
     2101 acgatgagtg ggtcaggccg atcgcggtgc gcggtacgcg gggcgcctgg tatcggactt
     2161 ggcggttggt gagcctggat ggcagcacct tcgatgtagc cgacgagaag gtcaacgaag
     2221 atgctttctc tcgccctgga gcgagccgcg gcgccagcgc gtatccgcaa atccgttttg
     2281 tatcgctggt ggagaacggt acgcacgtgc tgttcggcag ccagatggac agttaccgga
     2341 caggcgaaat cacgctggcc aaagcggtgc ttccgcgcct gggaaagggc atgctctgtc
     2401 tggctgatcg aaatttcttt ggcttcgaac tatggcaaca ggcgcgggga acaggcgcgg
     2461 atctgctttg gcggatgaaa aagaacatgc gcatggcctg cgagaagcgc ctgccggacg
     2521 gttcctattt gagccacgtg tatccttcgg agcgagacag agcat
//
LOCUS       DATMGX010000935         2029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_122540, whole genome shotgun sequence.
ACCESSION   DATMGX010000935 DATMGX010000000
VERSION     DATMGX010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122540"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1854
                     /locus_tag="VNR65_15280"
     CDS             304..1854
                     /locus_tag="VNR65_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWJ20081.1"
                     /translation="MRKGSRLEDKTAEDSAAVKEACTTPRLSGELLSKLNHVELEVRR
                     EAEKTLHFWDEAIADRSFLPAAINLANYLALRRLDLGEIQSALVELGLSSLGRSESHV
                     AATLQAVRASLARITGVAGVAYPMVGAWAEGRNLLALRKRELFGALGPAPAVMVTMPT
                     EAARDRDLVRSLLDAGMNCARINCAHDDPEVWQGIITHVRSAANELGRDCRILMDIGG
                     PKCRIESISPRKPKRLHVGDRFRVLREPEAAYGGSTPAITISFPEVVARLEVGSLIWI
                     NDGKIRGRVVEPAVGGWTVEVVGARGKGEKLRLEKGVDMPGVDLGLPPLSTDDLRDLD
                     FVAANADLVGFSFVQRPSDILLLDKHLADRLPGKPLPPLVLKIETAIAVQNLPRLIVQ
                     AAGRRPVAIMIARGDLAAALGFGRMAEIQEEMLWLCEAACVPVVWATQVLDDLVRDGL
                     PSRAEATDAAMAQRAECVMLNKGPHLVGAIHFLADIFARMDRHQVKKSARLGPLHSWP
                     SASLKLDP"
BASE COUNT          398 a          586 c          632 g          413 t
ORIGIN      
        1 tgttggcgag ggatgcctca acggggatac tgctcgcgtt gcgacggcgg tatcgctagg
       61 agacggcaca ataacctcga ttacgaaaca cgccatgcgt gaagcgctct ggttcggtgg
      121 agccgatgtt cgcgtcgccg cgatcgaaat ttgatcaaat tcgaatccac gcgactaatg
      181 gtcgtgaagg caaatgcaag cgatgggcag gacataagcg gatcaggttt cgcatttgga
      241 atccagcgat tgccgcctat cggacgaccc gatgaatatt ggctgataca gaggaccctg
      301 gaaatgcgga aaggctcaag acttgaggac aagactgcgg aagattccgc tgccgtaaag
      361 gaagcttgta caactccgcg cttatctggt gagcttctct ccaagcttaa tcacgtcgag
      421 ttggaggtgc gtcgcgaggc tgagaagacg cttcatttct gggacgaggc cattgccgat
      481 cgctccttcc tcccggcagc gatcaatctt gccaactacc ttgcactgcg acggctggac
      541 ctcggcgaaa ttcagtccgc tctcgtggaa ctgggtctgt cttcgctagg tcgcagcgag
      601 agccacgtgg cagcgacact ccaggcagta cgcgcctcgc tggcacgaat cacgggtgtc
      661 gcaggcgtgg cctaccccat ggtcggagcc tgggccgaag gccgcaactt gttggcgctg
      721 cgcaagcgtg agctttttgg cgctctcggg ccggccccag ccgtcatggt gacaatgccg
      781 accgaggccg cacgggatcg cgatctcgtc cgaagcctcc tggatgcggg catgaattgc
      841 gcccgtatca actgcgctca tgacgatccg gaagtatggc aagggatcat cacgcatgtc
      901 agaagcgcgg cgaacgaact tggccgcgat tgccgcatcc tgatggatat cggcggaccc
      961 aagtgtcgaa ttgagtcgat ttcgccaaga aagccaaagc ggctccacgt cggcgatcgc
     1021 ttccgagtgc tgcgtgagcc ggaagcggcg tacggcggct caacgcccgc catcacgata
     1081 agttttccgg aagtggttgc ccgtctggaa gtcggttccc tcatctggat caatgacggc
     1141 aaaatccgcg ggcgcgtggt cgagcctgcg gtgggcggtt ggacggtgga ggtcgttggt
     1201 gccagaggga aaggcgaaaa gcttcgactt gaaaagggcg tcgatatgcc cggcgtcgat
     1261 ctgggacttc caccgctgtc aacagacgat cttcgcgatc tggatttcgt cgccgctaat
     1321 gcggatctgg tcggcttctc gttcgttcag cggccctcgg acatcctact gctggacaaa
     1381 catcttgccg accggctgcc tggtaaaccg ctgccgccac ttgttctgaa aatcgaaaca
     1441 gccatagcgg tacaaaatct gccgcggctc atcgttcagg cggcgggccg ccgtcctgtc
     1501 gcaatcatga ttgctcgggg cgatctggcc gccgcgcttg gatttggccg catggccgag
     1561 attcaagaag aaatgctgtg gctttgcgag gcggcttgcg tccccgtggt ctgggcgaca
     1621 caagttctcg acgatctggt gcgcgacgga ttgccgagta gggccgaggc gaccgacgct
     1681 gctatggcgc agcgcgccga atgcgtgatg ctcaacaagg gaccacatct tgtcggggcc
     1741 atccacttcc tcgccgatat cttcgcccgc atggatcgcc atcaagtcaa gaagtccgct
     1801 cgccttgggc cgctgcattc ctggccatcc gcgagtttga agctggatcc ttaaatggcg
     1861 aaagttcgcc gctctgccgt ggagcgaaac gggccttcga gagatgcagg gccgtcatcc
     1921 gcccgggagc cgtcgctgat tacgccgcgc tcgccaggcg cacgctcaac gacaatcgtt
     1981 ttgatcgggc ctgtcgcccg ggctcactgc tttcatgatg atcgtggcg
//
LOCUS       DATMGX010000936         7027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_307866, whole genome shotgun sequence.
ACCESSION   DATMGX010000936 DATMGX010000000
VERSION     DATMGX010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307866"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1184
                     /locus_tag="VNR65_15285"
     CDS             222..1184
                     /locus_tag="VNR65_15285"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWJ20082.1"
                     /translation="MHLSIQDGCSLPKPFTDPAWTARGERRAVVPLKRLETVWFNTGT
                     LCNIDCANCYIESSPKNDRLSYLSLDDVVPFLDEMVRLPRPVSLMGFTGGEPFMNPGF
                     IAILEETLRRGFETLTLTNALKPMDHRKPDITRLAKRYGSRMRIRVSLDDYRADVHDA
                     ERGEGAFAYALDGLIWLSRAGVELEVAARFLSGEPEEDLRQGFGALFAECGISIDCED
                     DKSLLLLPEMRHGENPAEITEACWGMLRKSPDDVMCSGARMVVKRKGAPLPAVAACTL
                     LPYDKCFELGATLAEASRDVPLSHPYCSTFCVLGGGSCGSARHA"
     gene            1177..1959
                     /locus_tag="VNR65_15290"
     CDS             1177..1959
                     /locus_tag="VNR65_15290"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWJ20083.1"
                     /translation="MHDEALEAELTVPRFLKDRRFWLAIIALGVILAIHASGLPGSLT
                     LETLRANRQDIVTFVSENYLLSALGYVAIYVLVVALSLPSAVLLTLSGGFLFGAVAGT
                     MFTLIGATVGATVLFIFARTMIGEDAMLHFGKTGAKLASAVKANAWSYLLVLRLLPLF
                     PFFLVNVIPALAGVRLSTFVITTFFGIIPATIIFSLSGAGLGDVLDQGSSITIDSILT
                     PEIMAALAGLALLALAAIPLKRKFQNDTRGAARSAEYIGKAD"
     gene            2083..3933
                     /gene="typA"
                     /locus_tag="VNR65_15295"
     CDS             2083..3933
                     /gene="typA"
                     /locus_tag="VNR65_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWJ20084.1"
                     /translation="MTFRKARLGMNLRNIAIIAHVDHGKTTLIDVLLKQSGSFRDNQR
                     VAERAMDSNDLERERGITILAKVTSIQWKDTRINIVDTPGHADFGGEVERILNMVDGA
                     IVLVDASEGPMPQTKFVVSKALKIGLRPIVAINKIDKPDERHVEVLNEIFDLFANLDA
                     TEEQLDFPILYGSAKQGWMAVDPNGPKEDMAPLFDLVLKHVAPPRVGGGPFRMLATTI
                     EADPFLGRILTGRVTNGVVKPNMAVKAISRDGKLIDHGRVSKVLGFRGLERLPIDEGK
                     AGDIIAISGLTKATVADTICDPQESHALPAQPIDPPTLTMTFRINDGPFAGQEGDKVQ
                     SRVIRERLLREAEGNVALRIAETKDTDALEVSGRGELQLGILIETMRREGFELTVSRP
                     RVVFDTDPETGQLLEPIEEVIIDVDEEHAGAVVSKLSERRGELIEMRPSGGGRQRLIL
                     YVPTRGLIGYQGELLTDTRGTAIMNRLFHEYAPYKGEIQGRRNGVLISNSTGEAVAYA
                     LWNLEDRGPMMIDPQTRVYPGMIVGEHTRENDLEVNVLKGKQLTNIRTTSKDEAVRLT
                     PPMRMPLEKALAYIQDDELVEVTPKSIRLRKRYLDSNERKRMERAAAMAG"
     gene            complement(4853..5374)
                     /locus_tag="VNR65_15300"
     CDS             complement(4853..5374)
                     /locus_tag="VNR65_15300"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HWJ20085.1"
                     /translation="MRHKKGFGVRLLFASMFVAVLSVIVPATSFADKDVDPARLAAAK
                     ELLVVVGSAKQFDVVVPLITQQLESAFVNLKPDHAAEIKDVFRVIPEKFSQKKQELLD
                     QIAALYAEKLTADEINEIIKFYKTPVGTRFVQLQPELVQQSMVLGQAWGRKIGQEIDQ
                     EVRKQLKDRGVPI"
     gene            complement(5512..>7027)
                     /locus_tag="VNR65_15305"
     CDS             complement(5512..>7027)
                     /locus_tag="VNR65_15305"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HWJ20086.1"
                     /translation="KARTLAEAIEGADVFFGLSAAGALTKEMVASMAERPIIFAMANP
                     DPEITPEEVAQVRDDAIVATGRSDYPNQVNNVLGFPYIFRGALDVQARTINEPMKIAA
                     AHALAELARADVPDAVAKVYGRRLRYGPDYIIPAPFDPRLICEIPSAVAKAAMDSGVA
                     RRPIIDMEGYKVRLSARLDPAAGFINSIFESLRLNPKRVVFTEGEEEQVVRAASSFSA
                     QKLGHPILVGRDAQIRQALFLAGVNQEGLEVQNALHSEHNQEYAEFLYQRLQRDGYLY
                     RDCQRLVNNDRNVFAACMVAHGHADAMVTGVTRNWSTALQDVRRVLDPKPRRRVIGVS
                     VIISRGRVVLVADTSITAMPDAEELADIAVEAAGVASRFGMTPRVALLAFSTFGNPPS
                     ERADRVREAVRLLDQREVEFEYDGDMGADVALNRRAMALYPFCRLTDTANVLVMPAFH
                     AASISTKMLQELGGGTVIGPILVGLSKPVQIASLGSTDNDLVNMAALAAFDVTG"
BASE COUNT         1400 a         2100 c         2112 g         1415 t
ORIGIN      
        1 cggcgagcgt tacgccatcg gcacgcgcaa cggcttgacg ttcgaaggcg cttgggtctg
       61 gcaatggaaa aactggctcg accagcgcta tatggccaag ttcaagaatt tgtcagctag
      121 cgctttagcg cagtaacgac agtttgagaa tggcttggct gtacccttgc acatgcattg
      181 tgcaatgaat aacgcaatga tttgccccat cggaaaatct catgcatctc tcgatacaag
      241 acggctgcag ccttccgaag ccgttcacag accccgcctg gacggcgcgc ggagaaaggc
      301 gcgcggtggt gccgctgaaa cgccttgaaa ccgtttggtt caacaccgga acgctgtgca
      361 acatcgattg cgcaaattgc tacatcgaga gctcgccaaa aaacgaccgc ctctcctatc
      421 tctcgctcga cgatgtcgtg ccgttcctgg atgagatggt taggctgccg cggcctgttt
      481 ccctgatggg cttcacgggc ggcgagccat tcatgaaccc cggcttcatc gccattctcg
      541 aggaaacgct tcggcgcggc ttcgagacgc tgacgctgac caatgcgctg aagccgatgg
      601 accatcgaaa accagacatt acgcggctgg caaagcgtta tggttcgcgc atgcgcatcc
      661 gtgtttcgct cgatgactac cgcgccgatg tccacgatgc tgagcgcggg gagggtgcat
      721 tcgcttatgc ccttgacggg ctgatctggc tctcgcgcgc cggcgtggaa ctcgaggtcg
      781 ccgcccgatt cctgagcggt gagccggaag aggatttgcg gcaaggattt ggggcgcttt
      841 tcgctgagtg tgggatcagc atcgactgcg aggatgacaa gtcgctcctc ctcctccccg
      901 aaatgcggca tggagagaat cccgctgaaa ttaccgaagc ttgctgggga atgctcagga
      961 agtcgcccga tgatgtgatg tgctcgggcg cgcgcatggt ggtgaagaga aaaggcgcgc
     1021 ctttgcccgc cgtcgcggcc tgcacgcttc tcccttacga caagtgcttc gagcttggcg
     1081 ctaccttggc cgaagccagc cgggacgtac cactttcaca cccttactgt tccaccttct
     1141 gcgttctcgg gggcggttca tgtggatcgg cgcgacatgc atgatgaagc attggaggca
     1201 gagctgacag tgcctcgctt tttgaaagac cgtcggtttt ggctcgcaat aatcgcgctc
     1261 ggcgtcatcc ttgccattca tgcatcagga cttcccggct cccttacgct tgagacgctc
     1321 cgtgcaaatc ggcaggacat cgtcaccttc gtcagtgaga actaccttct ctcggctttg
     1381 ggctacgtcg ccatatatgt gctcgtggtg gcgctgtcgt tgccctccgc ggtccttctc
     1441 acgctctcgg gcggctttct cttcggcgcg gttgccggga cgatgtttac cctgattggc
     1501 gccactgtcg gcgcaacggt gctttttata ttcgcccgga caatgattgg cgaagatgcc
     1561 atgttgcact tcggaaagac cggagcaaag cttgccagcg ccgtcaaggc aaacgcatgg
     1621 tcttatctgc tcgtgcttcg gctgctgccg ctgtttccct tcttcctcgt caatgtcatt
     1681 ccggctcttg ccggcgtcag gctcagcact tttgtgatca cgacattctt cggcattatc
     1741 ccagccacga ttattttttc tctttcggga gcgggcctcg gagacgtgct ggatcagggg
     1801 agcagcatca caattgactc gatcctcacg ccggaaatca tggccgcgct tgcggggctc
     1861 gctcttctgg cgctcgccgc aattcctttg aaacggaagt ttcaaaacga cacgcgtggg
     1921 gcggcgcggt cggctgaata catagggaaa gccgactgac gcaggagaag tgatgccaca
     1981 agttagcgcc ggttcggtaa cccaacttgc gcagaaccag cgatgaccat tgacgcgggc
     2041 gcgcgtttgc ctcacatcta gcgcaaattc ccgtatcgca acatgacttt cagaaaggcg
     2101 cggctcggta tgaacttgcg caacatcgcc attattgccc acgtcgatca tggcaagacg
     2161 acgctcatcg acgtgctctt gaaacaatcc ggctcatttc gcgacaacca gcgcgtggcg
     2221 gagcgcgcga tggattcgaa cgatctggaa cgcgagcgcg gcatcacgat ccttgcgaag
     2281 gtcacttcca tccaatggaa ggacacccgc atcaacatcg tcgatacccc tggacacgcc
     2341 gatttcggcg gcgaggtgga gcgcatcctg aacatggtcg acggcgccat cgtgctcgtc
     2401 gatgcgtctg aagggccaat gccgcagacg aaattcgtgg tgtcgaaggc gctcaagatc
     2461 ggcctccgcc ccatcgtcgc catcaacaag atcgacaagc cggacgagcg tcacgtcgag
     2521 gtgttgaacg agattttcga cctcttcgcc aatctcgacg ccaccgaaga gcagctcgat
     2581 ttcccgatcc tgtacggctc agccaagcag ggctggatgg ccgtcgaccc gaatgggcct
     2641 aaagaggaca tggcgccatt gttcgatctc gtgctgaagc acgtcgcacc gccgcgcgtg
     2701 ggcggcggcc cgttccgcat gctggcgacc accatcgagg cggacccgtt cctgggccgc
     2761 atccttacgg ggcgcgtgac gaacggcgtg gtgaagccga acatggccgt gaaggcgatc
     2821 tcccgcgatg gaaaattgat cgatcatggc cgcgtgtcga aggtgctcgg cttccggggc
     2881 cttgagcgct tgcccatcga cgaaggaaag gcgggcgaca tcatcgccat ttccgggcta
     2941 acaaaggcga ccgtcgccga cacaatttgt gatccgcaag agtcacatgc gctgccggct
     3001 cagcctatcg atccgccaac gctgaccatg acgttccgca tcaatgacgg ccccttcgct
     3061 ggccaggagg gcgacaaggt gcaaagccgc gtcatccgcg agcggcttct ccgcgaggcc
     3121 gaaggcaatg tggcgctgcg gatcgccgag acgaaagata ccgacgcttt agaggtatcc
     3181 ggccgcggcg agttgcagct cggcatcctg atcgaaacca tgcgccgaga aggcttcgag
     3241 ctgacagtgt cgcgtccgcg tgtcgtgttc gataccgacc cggaaaccgg ccagttgctt
     3301 gagcccatcg aggaagtcat catcgatgtg gacgaggagc atgccggcgc ggtggtttcc
     3361 aagctgtcgg agcgcagggg cgaactcatc gaaatgcgcc cctcgggcgg cggccgccaa
     3421 cggctcatat tatacgtccc gacgcgcggg ctgatcggct atcagggcga gcttctgacc
     3481 gacacgcgcg gcacggcgat catgaaccgc ctcttccatg aatacgcgcc ttataagggc
     3541 gagatccagg gccgccgcaa cggtgtcctg atctcgaaca gcacgggcga ggccgtcgct
     3601 tacgcgctct ggaacctgga agaccgcggc ccgatgatga tagacccgca gacgagggtg
     3661 tatcccggca tgatcgtcgg cgagcacacg cgcgaaaacg atctggaagt caatgtgctc
     3721 aagggcaagc aactcaccaa cattcgcacg acgagcaagg acgaggcggt acgcctcaca
     3781 ccgcccatga gaatgccgct ggagaaagcg ctcgcctata tccaggatga tgagcttgtg
     3841 gaggtgacgc cgaagtccat tcggctgcgc aagcgctacc tcgattcaaa cgagcgcaaa
     3901 cggatggagc gcgcggcagc gatggctggt taggacgcgc tgaagggccg gatcgagcag
     3961 gtcgcgcccg gccatgggtt ggcggcgtat cacggcttca acgcggcctc ttcgatcgtc
     4021 gagaggaagc gcgccaagtc gcctcttgcg tctttccggg ccaagcgaag cgcgagccgg
     4081 attgtctcaa aacggacgcc aaattttcac cgcactggat acggcgggag cgccgttctt
     4141 ccgggcggcg agagcgtggc aatcttcaag cgcacggcgc aaaagccaag caacaaaggt
     4201 atacggatcg ttgccgtttc cgtgccggcc gtgcgtttaa ccaaaactgg gttaacagaa
     4261 gagcgccgga attatttccg ctcacattat gcttaaaaag ccgtttatcc tggcgcagaa
     4321 gaaaggcgcc gcgccctcga tgtgacggga aacaattgcg ctatcctgct gaaactaaac
     4381 agcctttcat cggcggtgac ctcccattaa atagaattta atgccggaat tattcccttt
     4441 actgggacgc tctaaagtcc gttaaatatg tttcgggtcc gcgttttaac catgaccgcg
     4501 cagcagtcgg cgcagcgcgc gcttccgttc ccgaaaactt atcaattata tcaatgagtt
     4561 ggctgtctta acctctcgtc aagacgcttt ctcctacgat ttgtggctga agcatccctc
     4621 ctgccaccgg agatgacgca agatgacgcc attcgcaccg tgcattcaaa aagcccgtcg
     4681 gcctcaaaat caggttccgt gtcggcgggg agcgtctctt tccggccaat gccgaggctt
     4741 gaaggagccc cttcaatgat acgagtcgaa cgctctgacg gttgttacaa tactcttcaa
     4801 aaacagccct tggccgggat aaagcccggc catcacgttg gcctatgcgg cctcaaatcg
     4861 gaacgccgcg gtccttcagc tgcttgcgga cttcctgatc gatttcctgg ccgatcttcc
     4921 ggccccaagc ctggccaagc accatgctct gctgcacaag ttccggctga agttgcacaa
     4981 acctagtgcc gacgggtgtt ttgtaaaatt tgatgatttc gttgatttcg tcggcggtca
     5041 gcttttcggc gtagagcgcc gcgatctggt ccagaagctc ctgctttttc tgcgagaact
     5101 tctccgggat gaccctgaaa acatccttga tctcagccgc atggtccggc ttgaggttca
     5161 caaacgcgct ctcaagctgc tgcgtaatca gcggcacgac gacatcgaac tgcttggctg
     5221 accccacaac gaccagcagc tccttggcgg cggcgagacg tgcgggatcg acgtctttat
     5281 ctgcaaagct ggttgcgggg acgattaccg acaaaaccgc gacgaacatt gaggcgaata
     5341 ggagccgcac cccaaatccc tttttgtgtc tcaagcgatg tcctttccgg tcggtttctg
     5401 tgtcgtgcct tttcgtaccg gcatcggcgg agcggcgcac ggcttttctc tgccgcccgc
     5461 acatcaagat gtggaagacc ttgcgccgcg ccggctcgct gatgacgggc ttcagcccgt
     5521 cacgtcgaaa gccgccagcg ccgccatatt tacaaggtcg ttgtcggttg agccgagcga
     5581 cgcaatctgc accggcttcg aaaggcccac gaggatcggc ccgataaccg tgccgccgcc
     5641 aagttcctga agcatcttgg ttgagatgct tgcggcgtgg aaggcgggca tgacgagcac
     5701 gttcgcggtg tcggttagac ggcagaacgg atagagagcc atggcgcgcc tgttcagagc
     5761 gacgtcggcg cccatatcgc cgtcatattc gaactccacc tcgcgctggt cgagaagcct
     5821 tacggcctcg cgaacgcggt ccgccctctc gctcggcgga ttgccgaagg tggagaaggc
     5881 gagcagcgcg acgcggggcg tcatgccgaa ccgggatgcc acgcctgcgg cctcgacggc
     5941 gatgtcggcc agctcttctg cgtcgggcat ggcggttatg gaggtatcgg cgacaaggac
     6001 caccctgccg cggctgatga tgacggaaac gccgatcacc cggcggcgag gcttaggatc
     6061 gagcacgcgc cggacatcct gaagcgccgt agaccaattg cgtgtgacgc ccgtcaccat
     6121 ggcgtccgcg tggccgtgcg cgaccatgca cgccgcaaac acgttgcggt cgttgttcac
     6181 gagccgctgg caatcgcggt agaggtagcc gtcccgttgc aggcgctggt agaggaattc
     6241 tgcgtactct tggttgtgct cggaatggag cgcgttttgg acctccaagc cctcctggtt
     6301 cacgccagcc aggaacaggg cttggcggat ctgcgcgtcg cgtccgacca ggatcgggtg
     6361 tcccagcttc tgggccgaga atgaactcgc cgcccggacg acctgctcct cctcgccctc
     6421 ggtgaagacc acgcgtttcg gattgaggcg aagcgattcg aaaatggagt ttatgaaccc
     6481 cgccgccggg tcgagtcgtg cggaaagcct gaccttgtaa ccttccatgt cgatgatcgg
     6541 ccgacgcgca acgcccgagt ccatcgcggc tttggcgacc gcggatggaa tctcgcagat
     6601 gagacgcgga tcgaagggag cggggataat gtagtcgggg ccataacgca agcgccggcc
     6661 gtagactttg gccaccgcgt cgggcacatc ggcgcgcgcc agttctgcaa gcgcatgggc
     6721 cgccgcgatt ttcatcggct cgttgatggt ccgggcctga acgtcgagcg cgccgcggaa
     6781 gatgtacggg aagccgagga cgttgttgac ctggttggga tagtccgagc ggcctgtagc
     6841 gacgatggcg tcgtcacgga cctgggcgac ttcctccggc gtgatttcgg ggtcggggtt
     6901 cgccatagca aagatgatag gccgctcggc catgctggcg accatctcct tcgtcagcgc
     6961 tccggcggca gaaagaccga agaagacatc tgcaccctca atggcttcgg caagcgtccg
     7021 cgctttc
//
LOCUS       DATMGX010000937         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_207964, whole genome shotgun sequence.
ACCESSION   DATMGX010000937 DATMGX010000000
VERSION     DATMGX010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207964"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNR65_15310"
     CDS             <1..210
                     /locus_tag="VNR65_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20087.1"
                     /translation="LMTIPFLPPNSDRYDAAVEDAIATCNGDLRGALRTLMLVNEQLE
                     SELVELQAAMAAFGTVERRTDQSLH"
     gene            378..569
                     /locus_tag="VNR65_15315"
     CDS             378..569
                     /locus_tag="VNR65_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20088.1"
                     /translation="MIILADQQLALQAVSDAQCILEEYLEPRPHNNERILDRLVDILA
                     RPELLVAVERLKRGSQADW"
     gene            complement(710..907)
                     /locus_tag="VNR65_15320"
     CDS             complement(710..907)
                     /locus_tag="VNR65_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20089.1"
                     /translation="MLHYRAYLLDEHHRVICAANLYCADEQAAKERAQQLLIDANDVE
                     LWQLDRRIAVFKSAGRPSADP"
     gene            complement(1141..1473)
                     /locus_tag="VNR65_15325"
     CDS             complement(1141..1473)
                     /locus_tag="VNR65_15325"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HWJ20090.1"
                     /translation="MPLYMYQAAYTAESWAAQVKNPQNRVESVGRQATEAVGGKMIGG
                     WYSLGHHDVILIADVPNIESMAAIAVAIAAGGAIKSSQTTALMTGAELVAALKKSEAV
                     VKGYKPAR"
     gene            complement(1509..1982)
                     /locus_tag="VNR65_15330"
     CDS             complement(1509..1982)
                     /locus_tag="VNR65_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20091.1"
                     /translation="MIIKPSVPWHTRSTHSRLAHVLERLGLALIGASCGLFVAGVSGI
                     DLIGSAQVVLAMMLYGATGFFLGISLPQAPPGHSGTSADRLELLTAAGTFLIAVATVV
                     SISSIVLGETVGARTAGIIGLSWVISATMQIAAGVIARIKLARAVETAKRALFFQ"
BASE COUNT          475 a          605 c          603 g          445 t
ORIGIN      
        1 ctgatgacca ttcccttcct gccaccaaac tctgaccggt atgatgcggc tgttgaagat
       61 gccattgcaa cttgcaacgg cgatctaagg ggggcactcc ggaccttgat gctggtgaac
      121 gaacaactcg aatcggaact agtggaattg caggccgcca tggcagcgtt tgggaccgtg
      181 gaacggcgaa ctgaccagtc gctgcattag tgcaccaagg ccagtcggaa acgcgcatag
      241 tttcccgccg caaacgcggg agccgccccc tcaggtcaaa aaacggactc aagagacata
      301 agcccattga accaaatgga accaatcgtg acttaatatc gttagcgata gttgttatcc
      361 gcgccaaggt gacaggcatg attattttgg ctgatcagca actagcactt caggccgtga
      421 gcgacgcaca gtgcattttg gaagaatacc tcgagcctcg cccacacaat aacgagcgta
      481 tccttgacag gttggttgac atactggcgc ggcccgagct tcttgtcgcc gtggagcgcc
      541 tcaagcgcgg cagtcaggcc gattggtaat ttaggcttcc ggtttggttt gcctagcgcg
      601 acttttcagc ctcgccttgg cggatgtctg atcagccacg gaagaactga tcgagcgacg
      661 cgggtcgcta caatttggcg cggtaaagtt gggaagccca tgacgcacgt caagggtctg
      721 cggaagggcg accagcagat ttgaagaccg ctatccggcg atcaagctgc catagctcaa
      781 cgtcgttggc gtcaattaat aattgctggg cgcgttcttt tgctgcctgc tcgtcggcac
      841 aataaagatt ggccgcgcat attacgcggt ggtgttcatc cagaaggtaa gcccggtaat
      901 gcagcatccc cacggcccca gccgtggaaa cattacttca tgaggagaag caagaccacg
      961 gaaatttcgt ggggacgtgg ttttatgtaa caggatgctc gctcgtgaat gtccaacgcc
     1021 tccgggcacg cagcctcgcc cgctcggccg ctcgaaacca ctcagagcga gcattcagct
     1081 tattgcacct gttggtggag cttctcggcg acgccagcag tgatcgaagc cggacgattt
     1141 ctatcgggcg ggcttataac ctttgacgac ggcctccgac ttcttcaggg ccgccacaag
     1201 ttcggcgccg gtcatcaatg cagtggtttg gctggacttg attgcgccac ccgccgctat
     1261 cgctacggcg atggccgcca tgctctcgat gttcggcacg tcggcgatca ggataacgtc
     1321 atgatggcct aagctgtacc aaccaccgat catttttcct ccgacggcct cagtcgcctg
     1381 ccgtccaacg gattcaacac ggttttgcgg gttcttcact tgcgctgccc atgattctgc
     1441 tgtgtaggct gcctgataca tatagagcgg catgttcgga ctcccttagc cgaaattctc
     1501 gtgacctttc actggaagaa gagcgcgcgc ttcgccgttt ccacggcacg ggctaacttt
     1561 attcgcgcga tgacgccggc ggcgatctgc atggtggcgc tgattaccca gctcaaaccg
     1621 atgatgccag ccgtgcgggc gcccacggtt tcacccagaa cgatactgga aatggacacc
     1681 accgtcgcca ccgcgatgag gaaagtgcca gccgcagtta gcagctccaa cctatccgca
     1741 ctggtgccgc tgtgaccggg gggagcctga ggcagactga tcccgagaaa aaagccggtg
     1801 gccccgtaaa gcatcatggc gagcacgact tgagccgaac cgatcaggtc gatgccactt
     1861 accccggcaa cgaacagccc acaggaggcc ccgatcaaag cgagtccgag ccgttctagg
     1921 acgtgcgcca acctgctgtg ggtcgagcgg gtatgccatg ggacgctggg tttgatgatc
     1981 atttaatcct cccgatatag agaacgcgtt tcggttactg aaaaaggaag agtaaaaagc
     2041 gccgccggtt gtatcgccgg gtccattacc ttcgagggga agtgaagggt aaccaatcac
     2101 ttaccccatg agaagcagct gtgcttac
//
LOCUS       DATMGX010000938         3836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_207976, whole genome shotgun sequence.
ACCESSION   DATMGX010000938 DATMGX010000000
VERSION     DATMGX010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207976"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VNR65_15335"
     CDS             complement(<1..610)
                     /locus_tag="VNR65_15335"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HWJ20092.1"
                     /translation="MKKTICVRLACVSAAALLTGLGAADQANAGGFAVREQSTYFQGS
                     GFAGTAAGGDISSMYWNSAAITTVPGFNAEASGTIIFPTSDVTATGGLLALADPYRTA
                     NIAVDTFVPAMYGTYQVSDRLYAGIAVNSPFGFATKPDDLLWAGAPLARTSQVRTLDF
                     NPTVAYKITPELSIGVGAQVEYMRVRLNNNGLGLLPTRTIDAD"
     gene            complement(992..1615)
                     /locus_tag="VNR65_15340"
     CDS             complement(992..1615)
                     /locus_tag="VNR65_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20093.1"
                     /translation="MNKLIELSDLPRVAQGPVLFWQQSLKNPLQVCSLFPSSPFVGRA
                     MTMVLGNRIDSHVIELGAGTGAITSQLIRNGVEPKKLTLIEIDAQLGGHLRRRFPDVD
                     VVIGPAQDLAKLWSERNGENVGAIVSTLPMRLFSKKLIYLVMKNSLNVLDDGGMFVQF
                     TYRQTSPVPPRVVKSLRLKAWRCTRVWLNLPPAAIWVYERETAPREI"
     gene            2018..3229
                     /locus_tag="VNR65_15345"
     CDS             2018..3229
                     /locus_tag="VNR65_15345"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWJ20094.1"
                     /translation="MTAELLDSFNSIANADVAGKRVLIRADLNVPMSGGQVTDATRIE
                     RLLPTFQGLLRRGAKVVILSHLGRPDGKPVAKYTLKPVAEKLSSLLGIQVFFGEDCIG
                     PAAKALVDGLSSGQIGMLENLRYHEGEEKNDASFAAELAKLGDIYVNDAFSVSHRAHA
                     STEGLARLLPAYAGPSMLAEIGALKLALDAPKRPVAAIVGGSKVSTKIDVLGHLAKKV
                     DYLIIGGGMANTFLFAKGSAVGKSLHEPGAVSTVEDILGKAAEAGCKIVLPVDAVVAG
                     ELKANVETAVVPVNEVPADKMILDVGPASVKEFAQTIANCKTLLWNGPVGAFEIPPFG
                     EATFELAREAAKLTQAGALTTIAGGGDTVAALNAAGVTDKFSYVSTAGGAFLEWLEGR
                     TLPGVAVLMRS"
     gene            3271..>3836
                     /locus_tag="VNR65_15350"
     CDS             3271..>3836
                     /locus_tag="VNR65_15350"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWJ20095.1"
                     /translation="MARITLRQLLDHAAEHGYGVPAFNINNMEQGISILEAAKETDSP
                     VIIQASRGARSYAGDLLLGKLVEGLESQYPTIPICLHQDHGNNEQTCASAIRIGYDSV
                     MMDGSLKGDGKTPADYDYNVGITSHVTEMAHWVGVSVEGELGCLGSLETGMGEKEDGH
                     GFEGALSREELLTDPDQAAQFVAATKVDA"
BASE COUNT          830 a         1074 c         1127 g          805 t
ORIGIN      
        1 agtcggcgtc tattgttcgc gttggaagca aaccaaggcc gttgttgttt agcctcaccc
       61 tcatatattc gacctgggcg ccaacgccga tgcttaattc aggtgtgatt ttgtaggcga
      121 ccgtgggatt gaaatctaat gtccggacct gggaggtgcg agcaagaggc gctccggccc
      181 agagaaggtc gtcaggcttt gttgcaaacc cgaacgggga attcaccgcg ataccggcgt
      241 aaagcctgtc ggagacctga taggttccgt acattgccgg aacgaaggta tcgactgcga
      301 tattcgccgt cctatagggg tcggccaatg ctagaagtcc gcccgtggcg gtcacatccg
      361 aagtgggaaa gatgattgtg ccgcttgcct cggcattgaa gccgggcaca gttgtgatgg
      421 ccgcagagtt ccaatacatc gagctgatat cgccgcctgc ggcagtaccg gcgaaaccgg
      481 agccctggaa ataggttgat tgctcgcgga cggcgaaccc gccggcgttc gcctgatcgg
      541 cggccccaag cccggtcaaa agcgcagcgg cgcttacgca cgccagccgg acgcaaatcg
      601 tctttttcat tttctccccc gtcgccgagc ggcagctcat ccccatgcgc gcctgattct
      661 cgaatactca cttagccatc tgattcgaag ccgtgacaat tggcacgcag atacgcgatc
      721 aggttgcatc aggtatgcaa aaggagggca gggtagagag gaatcagata ggcaggactt
      781 caaaaatctt cgtattcaca ataagatagc atgcataaaa gatggttcgg gaaagcggtt
      841 tctgccgagg cgtgtgtggc tattcgcgag gcaacttgct ctgctggcgc ccttttgtat
      901 ggaactgtgg cagagcggcc acccgaacgg ggcaccttcg cgcgcacttc ttcagcggtg
      961 ccgccaagat gatatatctg aaaattgcgc cttaaatttc tcgcggagcc gtttcccgtt
     1021 cgtagaccca gatcgcggcc ggcggaaggt tcagccatac gcgggtgcag cgccaagctt
     1081 tcaaacgaag cgacttgacc acgcgtggag gcaccgggct cgtctgacgg taggtgaact
     1141 gaacgaacat gccgccgtca tcgaggacgt tgaggctgtt cttcatgacg agatagatga
     1201 gcttcttcga gaaaaggcgc atcggcagcg tggatacgat agcgccaacg ttttcgccgt
     1261 tgcgctcact ccaaagcttg gcaaggtctt gcgccgggcc aatgactaca tctacatcgg
     1321 gaaagcgccg ccgcaggtgg ccgccaagtt gagcatcaat ctcgatcaag gtcagcttct
     1381 tcggttcaac gccgtttcga ataagctgac tggtaatcgc acccgtcccg gcgccgagtt
     1441 cgatgacgtg ggagtcgatc cggttgccca ggaccatcgt catcgcgcgt ccgacaaaag
     1501 ggctggacgg aaacaggctg caaacctgaa gcgggttctt tagcgattgc tgccagaaaa
     1561 ggacaggccc ctgcgccacg cgcggtagat cagataattc gatcagctta ttcatgaagg
     1621 tgacagcctc ttgtaccgct tggcgcgtaa aatctatcct tcaccatctg tctaggatgg
     1681 cagttgcatg cgttaacagg gccattctgg cgcctgagaa ccgggagctt ggctggattt
     1741 taacctgatc atgagataag gcgtcgtctt aacatttgca aacgacctgc tacgcgcgcc
     1801 gccgtcgcag ggggtaaata ccacgttaaa tatctctgac ttgtatattt cgcgcggaaa
     1861 tgtcacgctc cgtgcgagcg tcaacaaacc gcaatgcaac tcctgcaagt catcctaagt
     1921 gcgcttaaag cggcgcttat gcatggggga atggcgtttt aggcccagct aaaccagaaa
     1981 caggcccggc gatcgactgc gccgtaggga gaaaagaatg acagctgagt tgctcgactc
     2041 attcaacagc attgccaacg ccgatgtcgc cggaaaacgc gttcttattc gcgccgacct
     2101 caacgtcccg atgtcagggg gacaggtcac cgatgccacc cggatcgaaa ggcttcttcc
     2161 cactttccaa ggcctgctga ggcgcggcgc gaaggttgtc atactgtcgc atctcggccg
     2221 cccggacggc aagccggtgg cgaagtacac cttgaagccg gtggcagaga aattgtcttc
     2281 cctgctcggc atccaggttt tcttcggcga ggattgcatt ggacctgccg cgaaggcgct
     2341 ggtcgatggg ctttcgtctg gccagatcgg tatgctggaa aatctccgct atcatgaggg
     2401 cgaggagaag aatgacgcga gtttcgccgc ggaactcgcc aagctcggcg acatctatgt
     2461 gaatgacgcc ttctccgtct cccatcgcgc gcacgcatcc accgaagggc ttgcgcgttt
     2521 gctgcccgcc tatgccggcc cttccatgct cgccgagatc ggtgctttga agctcgccct
     2581 tgatgctccg aagcggcccg ttgccgcaat cgtgggcggt tccaaggttt cgacgaaaat
     2641 cgatgtcctc ggtcatctcg ccaagaaggt cgattacctc atcatcggcg gcggcatggc
     2701 gaacacgttt ctgttcgcga agggctccgc cgttgggaag tcgcttcacg agccgggcgc
     2761 agtttcgacc gttgaagata ttctgggcaa ggcggctgag gctggttgca agatcgtcct
     2821 gccggtcgat gccgtagtcg caggtgaact caaggcaaac gttgagactg ccgtggtgcc
     2881 ggtcaacgaa gttccggcgg acaagatgat cctcgacgta ggtcccgctt cagtgaagga
     2941 attcgcgcaa accatcgcga attgcaaaac ccttctttgg aatggccctg tcggcgcatt
     3001 tgagatacct cctttcggcg aagccacgtt cgaactggcg cgggaggctg cgaaactgac
     3061 ccaggccggg gccctcacca ccatcgcggg cggcggcgac acggtggcag cgcttaacgc
     3121 tgccggcgtt accgataaat tctcctatgt gtccaccgcg gggggcgcat ttctggaatg
     3181 gctcgaaggc cgcacgcttc ctggtgttgc ggtgcttatg cgctcttaaa accgaaccgg
     3241 caaaatcagt aacctatttg ggaggctcat atggcccgta ttacgctaag acaacttttg
     3301 gatcacgctg ccgaacacgg ctacggcgtg cccgctttca acatcaacaa tatggagcag
     3361 ggcatctcca tcctcgaagc ggcgaaggag accgacagcc cggtcattat ccaggcgagc
     3421 cgcggcgcgc gctcctatgc tggcgacctc cttctcggca agcttgtcga aggtctggaa
     3481 tctcaatatc ccacgatccc gatctgtctt catcaggacc acggcaataa tgagcagacc
     3541 tgcgccagtg cgatccgtat cggctatgac agcgtaatga tggacggctc gctgaagggc
     3601 gatgggaaaa cgccagccga ctacgattat aacgtaggca tcacctctca cgttactgag
     3661 atggctcatt gggttggcgt ttccgtggaa ggcgaactcg gctgcctcgg aagtcttgaa
     3721 acaggcatgg gcgagaagga agacggccac ggctttgaag gcgctctctc aagagaagag
     3781 ttgctgactg atccggatca ggcggcgcag tttgttgccg cgaccaaggt cgatgc
//
LOCUS       DATMGX010000939         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_60335, whole genome shotgun sequence.
ACCESSION   DATMGX010000939 DATMGX010000000
VERSION     DATMGX010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60335"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VNR65_15355"
     CDS             <1..257
                     /locus_tag="VNR65_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20096.1"
                     /translation="LSNLVRFPLIFISGIFVPIASLQGVARVAVYCSPITYLVNGFDL
                     GLLGSAAIPLLLGWAVLINFSIAFVLFARIFHRRNLMKAL"
     gene            complement(349..1419)
                     /gene="arsB"
                     /locus_tag="VNR65_15360"
     CDS             complement(349..1419)
                     /gene="arsB"
                     /locus_tag="VNR65_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HWJ20097.1"
                     /translation="MPEKLKARLSHLNELLTLWIFLAMVTGVLLGYLIPGIPAALSSL
                     QVGTTSIPIAIGLIVMMYPPLAKVRYEELGLVFRNTKVLGLALVLNWVVAPLFMFALA
                     VIFLRSYPEYMVGLILIGIAPCIAMVIVWNGLAGGDSEYAAALVALNSVFQVLFYSAY
                     AYFLITILPQWLGIFQEATVVNVSIGAIATTVAIYLGIPFVAGMLTRYAGLKLKGREW
                     YETRFVPKIGPVTLLALLYTIVIMFSLKGSYIIMLPLDVVRIAIPLLVYFVVMFFATF
                     YLCWRLRVNYAQSTTLAFTAASNNFELAIAVAVAVFGISSGQAFAAVIGPLIEVPVLI
                     SLVSVALWFSTRYFRQNGEAKH"
     gene            complement(1481..>2051)
                     /locus_tag="VNR65_15365"
     CDS             complement(1481..>2051)
                     /locus_tag="VNR65_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ20098.1"
                     /translation="AGFDCFLAANAVGVTGQVIGVDMTPEMLEKARANARKGSYSNVD
                     FRLGELEHLPAADNSVDVMISNCVINLTPDKAAVFREAFRVLKPGGRLAVSDMVLLKE
                     LPDVVKQSVAAYVGCLSGAIMKNAYVATIRAAGFDDVRMIEESSFPLDYMASDPSAQE
                     IRDSINLPPDELRDLADSVVSIKVAGKKP"
BASE COUNT          488 a          579 c          564 g          420 t
ORIGIN      
        1 tgctctcaaa cctcgtccgc tttcctctga tcttcatcag cggcatattt gtgccgattg
       61 cgagcctcca aggggtcgca agggtggccg tctactgttc gccgatcacg tacctcgtta
      121 acgggtttga cctcggcttg ctgggaagcg ccgcgatccc gctcctgctg ggttgggccg
      181 tccttatcaa ctttagcatt gcctttgtgc tgttcgcccg catttttcac cggcgcaacc
      241 tgatgaaagc cctgtgatca agcgcgagta cgcgtttccc gtcgattggc accaactcct
      301 gttgactgag tgccagctac aaaagaaaga ctgtgtatat gtccgtcgct agtgtttcgc
      361 ttccccattt tgcctgaaat atcgcgtcga aaaccacagg gcaacgctca caaggcttat
      421 gagaacggga acctcaatca gcggcccgat gacggcagca aacgcttggc ctgagcttat
      481 gccaaagacg gcgaccgcga ctgctatggc aagctcgaag ttattgctcg ccgcggtgaa
      541 tgcgagcgtg gtcgactggg cgtaattaac cctgaggcgc cagcacaggt aaaaggtcgc
      601 aaagaacatg acgacgaagt acacgagcaa cgggatggcg atccgaacca cgtcgagcgg
      661 gagcattatg atgtaacttc ccttcagcga gaacatgatg acgatggtat acagcagggc
      721 aagcaacgtc accggcccga tctttggcac aaaacgtgtc tcgtaccatt cgcgcccttt
      781 gagcttcagc ccggcatacc gcgtcagcat ccctgcaaca aaggggatgc cgagatagat
      841 tgccacggtc gttgcgatcg ctccgatcga cacattgacc acggtagcct cttggaagat
      901 gcccagccac tgcggcagta ttgtgataag gaagtaggcg taagcagagt agaagagtac
      961 ctgaaagacg gagttgaggg cgacaagcgc cgctgcatat tcagaatcgc cgccggcgag
     1021 gccgttccag acgatgacca ttgcgatgca cggcgcgatg ccgataagga tgagcccgac
     1081 catatattca ggatagctgc gcaaaaagat gacggccaac gcgaacataa agagcggcgc
     1141 gaccacccag ttcagcacca gtgctaagcc taagaccttg gtattccgaa agacgagtcc
     1201 cagttcctcg tagcgcacct tcgccagcgg gggatacatc atcacgatga gtccgatggc
     1261 gatcgggatg gaggtcgtgc cgacctgcaa gctgctcagc gcggcgggga tgccggggat
     1321 caggtagccg agaagcaccc ctgtgaccat cgctaggaat atccatagcg tcagcagctc
     1381 attcaagtgg gagagtctgg ccttaagctt ttctggcata gtagaacgga gatgaatctg
     1441 tcagcgcctt atcgatacaa atcggaccga gaacagacac ttaaggcttc tttcccgcca
     1501 ctttaatact gaccacagag tcggccaggt cacgcaattc atcaggaggc aggttgatgc
     1561 tgtcccggat ctcttgcgcg gacggatcgc ttgccatata gtcaagcggg aaggaggatt
     1621 cctctatcat gcgaacgtcg tcaaatcccg cagcccgaat ggtcgcaacg taggcattct
     1681 tcatgattgc cccggaaagg cagccgacat acgctgcaac tgactgctta acgacgtcgg
     1741 gcagctcctt aagcaaaacc atatcggaga ccgcaagccg accgccgggc ttcagtaccc
     1801 gaaacgcttc cctgaagact gcagccttgt cgggggtgag attgatcacg cagtttgaga
     1861 tcatgacatc gacagaatta tcggcagcag gcaggtgttc caattcaccc agtcggaagt
     1921 cgacgttgct gtagctgccc ttacgcgcat tcgcccgcgc cttttcgagc atttcaggcg
     1981 tcatatcaac gccgatcacc tgccctgtaa caccgaccgc gtttgcagcg aggaaacagt
     2041 cgaatccggc t
//
LOCUS       DATMGX010000940         2382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_219261, whole genome shotgun sequence.
ACCESSION   DATMGX010000940 DATMGX010000000
VERSION     DATMGX010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219261"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1136
                     /locus_tag="VNR65_15370"
     CDS             336..1136
                     /locus_tag="VNR65_15370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ20099.1"
                     /translation="MPLLEARHLTKTFSSGQTMFGAGTSGQVRAVNDVSLAIEAGETL
                     GLVGESGSGKSTLGRMVLRLIEPDSGEVFFDGHDVLRAGGAQLRRLRRDMQIIFQDPF
                     GSLDPRMTVEQIVCEPMAIHGTDGRGDRRQRAAEVMRVVGMDSSALQRYPHEFSGGQR
                     QRVGIARALVLRPRFIVADEPVSALDVSVGSQIVNLLKQLQRDFSLTYLFISHSMPIV
                     RYLANRIAVMQRGELVEVGATAQITTAPRHPYTRSLLAATPEMEFSSR"
     gene            complement(1123..1665)
                     /locus_tag="VNR65_15375"
     CDS             complement(1123..1665)
                     /locus_tag="VNR65_15375"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWJ20100.1"
                     /translation="METLYYSHLQSPVGPLLIGVSDEALVALEFDRGLPQKIGGRQIR
                     WEQSEVRTRPVRQQLQEYFSGQRRNFDLALDLRGTEFHKRCWQQLLQIPYGETCSYAD
                     IARAVGSPKSFRAVGQANHHNPIAIIVPCHRVLAAGFYLGGYGGGLPVKAYLLRLEGA
                     SFRERSEASDETKQLAFSAS"
     gene            complement(1784..2272)
                     /locus_tag="VNR65_15380"
     CDS             complement(1784..2272)
                     /locus_tag="VNR65_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20101.1"
                     /translation="MAVLIVLTTAASLPSAAKVRGPSTPEERARAVEYVRSLEENPLA
                     KDGLAKRMWLTEWIVEVPDLTVNACCKELESLDKVNNTYSNQLRMQMVYSQAAFLLQH
                     PEEKNAATIQAAGLAGTLRAYRAIQRFDPTAKYPLLDNLISLEKQGKLQQYVQKKKCK
                     DN"
BASE COUNT          473 a          702 c          720 g          487 t
ORIGIN      
        1 cgccgcagtg gcagggttct cgtcgcgcta cgcgcagaca tggcgattat gctgtcaagt
       61 ttgcagacaa aagaaatgca agccggaacg aagcgtccgg cccgcaggtt actttcagtt
      121 gcagttggtt gcggagggca attcgcaact ggggaggaaa ctgtgaatca ataaacagag
      181 atcacggaga aaactcgata gaaaagtccg tgtcctccgt gccctccgtg gtgaatgatt
      241 ttcgacggcg cgaagtcgga cactccctat aacgacaacc accctccgcg ccaactactg
      301 ctcaccatta cgccccattt gttaccatcg ccagcatgcc cttgctggaa gcccgtcacc
      361 tcaccaagac cttcagctcc ggtcaaacca tgttcggagc ggggacatcg gggcaggtgc
      421 gcgccgtaaa tgacgtgtcg ctggcgatcg aagccggaga gacgctggga ttggtcggcg
      481 agtccggttc cggcaagagc acattggggc gaatggtgct gcgactcatc gagccggatt
      541 cgggtgaggt gttcttcgat ggacacgacg tgctgcgcgc cggtggagct caattacgcc
      601 gcttgcgtcg cgacatgcag atcatcttcc aggatccgtt cggctcgctc gatccccgca
      661 tgacggtcga gcaaattgtc tgcgagccca tggccatcca tgggaccgac ggccgtggcg
      721 accgccgcca acgcgcagcg gaggtgatgc gtgtcgtggg aatggatagc tcagctctcc
      781 agcgttaccc ccacgaattc agcggagggc agcggcaacg cgtcggcatc gcgcgcgccc
      841 tggtgttgcg gccgcgattc atcgtcgccg acgagcccgt ctcggcgctg gatgtgagcg
      901 ttggttccca gattgtcaac ctgctgaagc aactgcagcg cgacttctcg ttgacctacc
      961 tcttcatctc gcactcgatg cccatcgtgc gctatctcgc gaaccgaatc gcagtcatgc
     1021 agcgcggaga gctggtcgag gtaggcgcca ccgcgcagat cacaactgcg ccgcggcatc
     1081 cctacacgcg cagtctgctg gcagctactc cggagatgga attcagctcg cgctaaaggc
     1141 gagctgcttc gtctcatcgg aggcctcgga acgctcgcgg aacgatgcgc cttcgagccg
     1201 caggagatac gcttttacgg gaagtccgcc gccgtatccc ccgaggtaga agccggcggc
     1261 cagaacgcga tggcagggca cgatgatggc gataggattg tgatggttcg cttgtccgac
     1321 ggcgcgaaag ctcttgggac tgcccacggc gcgtgcaatg tcggcgtacg aacaggtttc
     1381 gccgtagggg atttgcagca gttgctgcca gcagcgcttg tggaattccg tgccgcggag
     1441 atcgagcgcg agatcgaagt tgcggcgctg gccggagaag tattcctgca attgctggcg
     1501 aacggggcga gtgcgaactt cagattgttc ccatcgaatt tgccggccgc caatcttttg
     1561 cggcagccca cgatcaaatt ccagcgcgac cagcgcctcg tccgagacac cgatcagcag
     1621 cggccctacg ggcgattgca ggtgcgagta atagagcgtt tccatggctc gtctccgttc
     1681 cggcgacgcc ttttttcagc agcctctcta cgctgcgcga ttaccgcaaa cacgacgaag
     1741 ggcgcgcccg gcgcgccctt ggaagtaaat agaagctgac gttctaattg tccttgcact
     1801 tcttcttctg cacgtactgc tgcagcttgc cttgtttttc cagcgagatc agattatcga
     1861 gcaaaggata cttcgccgtg ggatcgaagc gctggatggc acggtacgcg cgcaaggttc
     1921 ccgccagccc cgccgcctgg atggtcgccg cgttcttttc ctctggatgc tgcaacagga
     1981 atgctgcctg agaataaacc atctgcatcc gcaactggtt ggagtacgtg ttgttcactt
     2041 tatcgagcga ctccaactct ttgcagcagg cattgacggt gaggtcggga acctccacga
     2101 tccactcggt cagccacatt ctttttgcga ggccatcttt ggcgagcgga ttctcttcca
     2161 gactgcgcac gtactcgacc gcgcgcgccc gttcttcagg cgtggaaggg ccccgcacct
     2221 tcgccgctga aggaagcgat gccgcggtgg tgaggacgat caggactgcc aaaatcttgc
     2281 gtaccataat gccttctccc cttgaagtgc acgggtgggc cagatgagtg ttgagatcat
     2341 actccgattg ggggtaaaga aagcaagcgg gaattgaatt cg
//
LOCUS       DATMGX010000941         2763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_387762, whole genome shotgun sequence.
ACCESSION   DATMGX010000941 DATMGX010000000
VERSION     DATMGX010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387762"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VNR65_15385"
     CDS             complement(<1..1301)
                     /locus_tag="VNR65_15385"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HWJ20102.1"
                     /translation="MYHYVVALATIVLLVLAYRRLPVNPTTVALTFLLVVLLLASRWG
                     LAIATTTAIVAALAFNYYFLPPIHTFTIADPQNWIALLAFLTSAIVASRLAERARREA
                     LNATRRRKEVERLYSLSQQLLATDNVMELLNSIPHYVRDAFGLKAAAMFLPKRKAVYR
                     TGVDHPELSEEQLEAVSGRGELVVDEEQQLCFVPLRIGVKPVGSLGLEGAVLSRETLE
                     AVGSLIAISIERAGAVETLTRAEAARESETLRSALLDSVTHEFRTPLTSIKASVTTLL
                     STPQIDSEGQKELLTVIDEETDRLNRLVGEAAEMAQLDAQQVELRRETYTVRRVVEAA
                     LEKSKQLLAGREVKLQLPESLPPVRIDLERIAEVLVQLLENAAKYSPPGTPITISAEP
                     AGKMVDLSIADRGPGIDDMEQSLIFEKFYRGRDQRYRVQGTG"
     gene            1435..2559
                     /locus_tag="VNR65_15390"
     CDS             1435..2559
                     /locus_tag="VNR65_15390"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWJ20103.1"
                     /translation="MQKTPEQWLEETAAVKKSGVFKLFLGYAPGVGKTYAMLSEAIRR
                     QSRGEDVVIGVIETHGRKATQEIASRLETVPRKKIDYKGTLFEEMDVDAILARHPQVA
                     LVDELAHTNIEGSKHHKRYEDVFELLDAKIDVVSTMNVQHLESVTPMVHKITGVLVRE
                     TVPDWVLKRVDEIVMSDVTPEALQTRMRRGDIYPMERVDRALGNFFRSSNLIALREVA
                     LRHLAQVVDRSLERYLDTESPGRDLNVAERIAVGVSSNPAAQYLVARASRMAQAINGE
                     LFVLYVDTGVDADDPERQRALQANLHFAENLGAKIVRLKGKDVAQAMAEFVREKHITQ
                     MIFGHSATSGWRKYLFMSAVHRFLRDAPAVDVHIVKQQST"
BASE COUNT          579 a          771 c          854 g          559 t
ORIGIN      
        1 cctgtgcctt gcacgcgata acgctggtcg cgtccccgat agaacttctc gaagattagc
       61 gactgctcca tatcgtcaat gccggggccg cggtcggcga tgctgagatc caccattttt
      121 ccggctggct cagcgctgat ggtaatcggc gtaccaggag gcgagtactt ggccgcgttt
      181 tccagcaact gcaccaggac ttcggcaatg cgctccaggt caatgcggac ggggggcaac
      241 gactcgggaa gttgtagctt cacttcgcgt ccggcaagca gctgcttgga tttttccagc
      301 gcggcttcga cgacccgccg gacggtataa gtctcgcgac gtaattccac ctgctgtgcg
      361 tccaactggg ccatctccgc cgcttcgcct accaggcggt tgagccggtc ggtttcctcg
      421 tcgatgacgg tgagcaactc tttttgccct tcggaatcga tttgcggggt tgaaagcagc
      481 gtcgttacgg aagccttgat ggacgtgagg ggcgtccgga actcgtgagt gacgctatcc
      541 agcagggccg agcgcagcgt ttcgctttcg cgggccgctt ccgcgcgggt gagagtttcg
      601 accgctccgg cgcgctcgat ggaaatcgcg atgaggctgc cgacggcttc cagggtttca
      661 cgcgacagta ccgcgccttc cagccccagg ctgcccaccg gcttgactcc gatgcgcaag
      721 ggcacgaagc acagctgctg ttcctcatcc acgaccagtt cgccccttcc acttacggcc
      781 tcgagttgct cctcgctgag ttcggggtgg tccacgccag tgcgatagac cgctttgcgt
      841 ttgggcagaa acatcgccgc cgcctttagc ccgaaagcat cgcggacgta atgaggtatg
      901 gagttgagca attccatcac attatcggtg gccagcaatt gctggctcaa ggagtacagc
      961 cgctccactt ccttgcgacg ccgggtggcg ttcagcgcct cacggcgggc ccgctcggcg
     1021 agccggctgg ccacgatggc gctggtcaga aaagcgagta aggcaatcca gttctgcggg
     1081 tcggcgatgg tgaaggtatg gatcggcgga aggaagtagt agttgaacgc cagggcggcc
     1141 acgatggcag tcgtggttgc gatggccagt ccccagcggc tggctaacaa gagcaccacg
     1201 agcaagaagg tcagggcaac ggtcgtagga ttcaccggca gccggcgata ggcgagtacc
     1261 aaaagcacaa ttgtcgccag ggcaacgacg tagtgataca tctgcgtttt cagtcttcgc
     1321 ccagaaacag cgaaggacga ctgccggttg tgttcgtcgc tagatggtcg gggattcacg
     1381 ccatgattgt atcgaatcga cttttgcgtg cccctgccgc agaatagtat cgccatgcaa
     1441 aaaacccccg agcaatggct ggaagaaacc gcagcggtaa agaaaagcgg cgtcttcaag
     1501 ctgttcctgg ggtatgcgcc gggcgtgggc aagacctatg ccatgctgag cgaagccatc
     1561 cggcgccaaa gtcgcggcga agatgtcgtc atcggggtga tcgagacgca cgggcgcaag
     1621 gcgacacagg aaatcgcctc gcgcctggag accgtcccgc gcaagaaaat cgactacaaa
     1681 ggcacgcttt ttgaagagat ggatgtggat gccattctgg cgcgccatcc gcaagtcgcg
     1741 ctggtggacg agttggccca caccaacatc gagggcagca agcaccacaa gcgctacgaa
     1801 gatgtgttcg agttgttgga cgccaagatt gacgtggttt cgaccatgaa tgtgcagcac
     1861 ctggagagtg ttacacccat ggtccacaaa atcaccggtg tgctggtgcg cgagaccgtg
     1921 cccgattggg tgttgaagcg cgttgatgaa atcgtgatgt cagacgttac tcccgaagca
     1981 ttgcagacgc gcatgcggcg cggcgatatt tatccgatgg agcgtgtaga ccgcgccctg
     2041 ggcaactttt ttcgttccag taatctgatt gccttgcggg aagtcgccct gcgccactta
     2101 gcgcaggtgg tggaccggag cctggaacgg tatctcgaca cggaatcgcc cggccgcgat
     2161 ctgaacgtgg ctgagcggat tgctgtcggc gtcagctcca atccggcggc gcagtatctg
     2221 gtggcccggg cttcccgcat ggcacaagcg atcaacggcg aactgttcgt cttatacgtc
     2281 gataccgggg tggatgccga cgatccggag aggcagcgcg cgctacaggc gaatcttcac
     2341 tttgcggaaa atcttggggc aaagattgtg cggctcaagg gtaaggacgt tgcccaggcg
     2401 atggcggagt tcgtccgtga gaagcacatc acgcagatga ttttcggaca ttccgcaacc
     2461 agcggctggc gcaaatacct tttcatgtcg gccgtgcatc gcttcctgcg cgacgcgccg
     2521 gcggttgatg ttcacattgt gaagcagcag tcgacttgaa gcttaagttt cgagtttcga
     2581 gtttccagtt tcaagctcca gaacctagcc gcgggatagc gacaggaacc acagtgccta
     2641 taatcacagg agttcattgg taaaactgag gaactgctgc gaatgttgaa cggattgatg
     2701 ggctccctag cacgataact tgaaactcga aactcgaaac tcgaaacttg aaactcgaaa
     2761 ccg
//
LOCUS       DATMGX010000942         2594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_85156, whole genome shotgun sequence.
ACCESSION   DATMGX010000942 DATMGX010000000
VERSION     DATMGX010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85156"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VNR65_15395"
     CDS             <1..278
                     /locus_tag="VNR65_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20104.1"
                     /translation="AMHALFEIADVVLVGLDRPHNVGDGIQQLARERADALDGLAGRA
                     SGDPLMRDFAKDSDLRKAGADVVVQIAGDPGTDALHFEQPSYFLAQK"
     gene            421..780
                     /locus_tag="VNR65_15400"
     CDS             421..780
                     /locus_tag="VNR65_15400"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HWJ20105.1"
                     /translation="MRRQERLLQRLLRATENMVQGGLSETSRRCGNPNCICQRDPKRL
                     HGPHLYITYRIDGKSCSLYVPPEHAKTARRAQQAWAAFWKIGCSLAALNREQLRRQWQ
                     QKKKTRTATPTKEVVHD"
     gene            773..2206
                     /locus_tag="VNR65_15405"
     CDS             773..2206
                     /locus_tag="VNR65_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057901383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HWJ20106.1"
                     /translation="MIEARRAQLSFGDGLIAEEVADLWEDWMRPADQVLDDDQLLSTV
                     YEALTRRRPKSRTRGRKGTPAEVVLRLLLLKHIRNWSYEVVEREVRANLVYRSFTRVG
                     GGKVPDDTVMNKWALALGPEVIENLHKRVVTIAQEKKVVEGRKMRVDTTVVETNIHYP
                     TDSSLLGDGVRVLTRAMKRIAAIAGNVGAKLRDRSRSVKYRILEIGRAARSKGGAGKE
                     KLQQGYGRLLASVSRVVGQAKRFSREIASGVKKSSDVFQQAALDGLRKELDTMVPRAE
                     QVMRQARARIFGGDTHVSEKLVSIFEPSTEVIRKGKASKPTEFGKMVKIQEAENQIIT
                     GYSVYEKRPYDSALLIPAIESHEEQLGRVPRLVAGDAAFYSASNEKAAHEKGVKRVCV
                     PNRNTKSTERKREQKKRWFRNGQKWRTGCEGRISVVKRRHGLNRCRDKGDRGMQRWVG
                     LGVIGDNLINIGRVLTRREEATTAVAE"
BASE COUNT          582 a          758 c          786 g          468 t
ORIGIN      
        1 aagcgatgca tgcgttgttt gagatcgctg acgtggtcct tgtggggctg gatcgcccac
       61 acaatgtcgg cgatggaatc cagcaactcg cgagagagcg cgccgatgcg ctcgatgggc
      121 tcgccggccg tgcctccggc gacccgctga tgcgcgactt cgctaaggat agcgatctgc
      181 gaaaggctgg agccgatgtc gtcgtgcaga tcgctggcga tccgggtacg gacgcgctcc
      241 atttcgagca gcctagctac ttcctggcgc aaaaatgacc ggctaagccg ttcaatgtca
      301 atccgcgagg cggcgcactt ctgcatggtc gtacaaatcc gcgtcccaga acatttttga
      361 ccgcccatgt catttttctc ttgacatgaa aaccagaccg ccatatctta atattaaatg
      421 atgcgtaggc aagagcgact ccttcaacga ctcctccggg ccaccgaaaa catggtccag
      481 ggcgggctgt cggagacctc ccgccgttgc ggcaatccca actgcatctg ccagcgcgat
      541 cccaagcgcc tgcatggacc gcacctgtat attacctacc gcatcgacgg aaagagttgc
      601 tccctgtatg tccctccgga gcacgccaaa accgctcgcc gggcgcaaca ggcctgggct
      661 gctttttgga agatcggttg ctccctggct gccctgaacc gcgaacaact gcgacgacaa
      721 tggcagcaga aaaagaagac gcgcacggcg acaccgacca aggaagtcgt ccatgattga
      781 ggctcgccgc gcccagctca gcttcggcga cggcctcatt gccgaagagg tcgccgactt
      841 gtgggaagac tggatgcggc ccgctgacca ggtgctggac gatgatcaac tgctctcgac
      901 cgtttacgaa gccttgacca gaaggcgccc caagagccgt acccgtggga ggaagggcac
      961 gccggccgaa gtcgttctgc gattgctttt actcaagcac atccgcaact ggagctatga
     1021 agtggtggag cgcgaggtcc gcgccaacct ggtctaccgt tccttcacgc gggtgggcgg
     1081 cggcaaggtt cccgacgaca ccgtcatgaa caagtgggcg ctggccctgg gaccggaagt
     1141 gattgagaac ctccacaaaa gggtggtcac gattgcccaa gagaagaagg tcgtggaggg
     1201 gcgcaagatg cgcgtcgata cgaccgtcgt tgaaaccaac atccactatc cgaccgacag
     1261 cagtttgttg ggcgacggcg tgcgtgttct gacgcgcgcg atgaagcgga tcgccgccat
     1321 cgctggaaac gtaggggcca aactgcggga ccgcagccgg agcgtgaagt accggattct
     1381 ggagattgga cgggccgcac gcagcaaggg tggcgccggc aaagagaaat tacagcaggg
     1441 gtacgggaga ttactcgcgt ccgtctcgcg agtggtggga caggcgaagc gcttctcgcg
     1501 ggagattgcc tcgggagtca agaaatcctc ggatgttttc cagcaagccg cccttgatgg
     1561 cctgcgcaaa gagctggaca cgatggtgcc gagggccgag caggtgatgc ggcaagcccg
     1621 tgcacgaatc ttcggtggcg atacacacgt gtccgagaag ctggtcagca tcttcgagcc
     1681 gagcaccgaa gtcatccgta agggcaaggc gagcaagccc accgagttcg gcaagatggt
     1741 gaagatccag gaagccgaaa accagatcat cactggttat tccgtgtatg agaagcggcc
     1801 ctacgactcg gcgctgttga tcccggcaat cgagtcgcac gaggagcaac tgggccgcgt
     1861 cccgcgcctg gtcgccggcg acgcggcctt ctactcggca agcaatgaaa aggcggcgca
     1921 cgaaaagggc gtcaaacgcg tctgcgttcc caatcgcaac accaaaagca ccgaacggaa
     1981 gcgggagcaa aagaaacgct ggttccgcaa cggacagaaa tggaggaccg gctgcgaagg
     2041 gcgcatcagc gtggtgaagc gccgccacgg gctcaaccgc tgccgggaca agggtgatcg
     2101 tggcatgcag cgctgggtcg gactcggtgt aatcggcgat aacctgatca atatcggccg
     2161 ggtcctcacc cgcagggagg aagctactac tgccgtcgcc gaataattgc cgacgtcacc
     2221 caattattca cgggcccacc ggttctccgg tgggcctttt tgtgtctgtc tgatcagctc
     2281 cgtcgacccg cgcggagcgc gcttttcttt gaaaacgcat ttttgcgcca ggaagtaact
     2341 aatctctcgg aatcggaaag caatcattac gtgatgcttt gatagtcgcc atcggccgat
     2401 ctctgcactg tctacaagcc ttgtaaacat gcaatgacgc tgcaattgca gggactggaa
     2461 aggacggaca ccgagcacca gcacaaacat aacttcatat cgcaagccgt ggcgcgcgct
     2521 ctcctgtgtg cagcctcgat actcttcgac gcttcttcgc ccgaaaaacc aacgtcgact
     2581 ttccacgtag gagt
//
LOCUS       DATMGX010000943         2830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_138175, whole genome shotgun sequence.
ACCESSION   DATMGX010000943 DATMGX010000000
VERSION     DATMGX010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138175"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VNR65_15410"
     CDS             <1..1062
                     /locus_tag="VNR65_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20107.1"
                     /translation="LSAIAVVDNEYGYCFGAHLNFDPSLDRKTIQAGVEKSGDLSKPH
                     PHRRFARLWIDADHQEASAKSMIAKRRKLGLPAEIDETYTTAMMRDDVESPDTPSPTT
                     RLPEYGMQIHGEYTMYGCFFFLKRLLGNVQKWRFFLDQDPGLRAACLAAFRHEIQGRT
                     ADAFYVGVAKDLTVDEKRQKYLEAKVRFEQAQAAHPAMKPNEVKLMLIKERLATMTPH
                     GKWEDRWLAHPFPTMGEPEKAVAYLTDFGDYDEDHRAWLYNKASLHGVNSFFNQVRRR
                     LSLLERPIHSKTNRGRIWNGYSPYDPGNVIKALDIMRIVHNYILTGKDGKTPAERLGL
                     AQAPLDYEDIIYHPCPNVK"
     gene            complement(1055..2227)
                     /locus_tag="VNR65_15415"
     CDS             complement(1055..2227)
                     /locus_tag="VNR65_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HWJ20108.1"
                     /translation="MSHGQYVFAQLMDHAPLTTFRRCVARYGGDYKIKHFSCLDQFRC
                     LAFAQLTWRESLRDIEACLRAQSAKLYRLGFRSPQISRNTMANANAERDWRIYADFAQ
                     HLIRMARELYADETLPGLEGIDTVYALDSSTIDLCLSVFPWAPFRTTKAAIKLHTLLD
                     LRGNIPSFIFISDGKMHDVNILDHLAPEAGAFYVMDRGYVDFKRLAKLDGAGSFFVTR
                     AKSNMKAKRRYSRPVDRTTGLICDQTIVLTGFYTRQGFDRPLRRVKFNDPETGKSLVF
                     LTNNFALPALTIARLYKYRWQVELFFKWIKQHLRIKAFFGTSENAVKAQIWSAVCVYL
                     LVAIVKKRLAVKASLYEMLQILSLTLFEKTQLLQLFSTIPLQMSEPTTANQLNLFT"
     gene            complement(2495..>2830)
                     /locus_tag="VNR65_15420"
     CDS             complement(2495..>2830)
                     /locus_tag="VNR65_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20109.1"
                     /translation="SSCVLLYKAPLSETAKSRLDILRQVEDGVVIAEEDLRLRGGGEV
                     LGPRQSGDPGFRLAQWPQAASLVDRASDLARYLLLRDPHLKSEQGLAARRCLILFERD
                     DAMRLLQAG"
BASE COUNT          672 a          800 c          787 g          571 t
ORIGIN      
        1 ctatcggcta ttgccgtggt ggacaacgaa tatggctatt gctttggggc ccacctcaac
       61 ttcgaccctt cgctggatcg caaaacgatt caggccggag ttgagaagag cggcgacctt
      121 agcaaacctc atccgcatcg ccgctttgcc cgcctctgga tcgacgccga tcaccaggaa
      181 gccagcgcca agtccatgat cgcgaagcgg cgcaaactag gcctgccagc agagatcgat
      241 gaaacttata ccacagcgat gatgcgggac gacgttgaaa gtcccgatac gccatcgccc
      301 acgacgcggc ttcccgaata cggcatgcag atccacggcg agtatacgat gtacggctgc
      361 tttttcttcc tcaaacgctt gctgggcaat gtccagaagt ggcgtttctt ccttgatcaa
      421 gacccaggtc tcagggcggc ctgtctggcc gcattccgcc atgaaattca aggccgtaca
      481 gcggacgcct tttatgttgg cgtcgccaag gatttgacgg tggatgagaa gcgtcaaaaa
      541 tacctcgaag cgaaggtgcg gttcgaacag gcgcaagccg cgcatccggc catgaagccc
      601 aacgaggtca agctgatgct tataaaagag cgtctggcca ccatgacgcc gcacgggaag
      661 tgggaagacc ggtggcttgc ccatcccttc ccgacgatgg gcgaaccgga aaaggctgtc
      721 gcctatctca ccgatttcgg ggattacgac gaggatcatc gggcctggct ctacaacaag
      781 gccagcctgc acggcgtcaa ttcgttcttc aaccaagtgc gccggcggct ttccctgctt
      841 gaacggccta tccactccaa gacaaacaga ggccggatct ggaacggcta ctctccgtat
      901 gatccaggca atgtcataaa agctctcgat atcatgcgca tagtccacaa ctacatcctg
      961 accggcaagg atggcaagac gccagctgaa cgtctcggct tggctcaggc gccgcttgac
     1021 tatgaagata taatttacca cccgtgtccc aacgttaagt gaatagattc aattggttgg
     1081 ccgtggttgg ttcagacatt tgcaacggga tggtggaaaa gagctgcaaa agctgggttt
     1141 tctcgaaaag cgtgaggctc agaatctgta gcatttcgta gaggctggct ttgacggcga
     1201 ggcgtttttt gacgatggcg accagcagat agacgcagac cgccgaccag atttgcgcct
     1261 tgaccgcgtt ctcggacgtt ccgaaaaacg ctttgatccg caggtgttgt ttgatccatt
     1321 tgaagaacag ttcaacttgc cacctgtatt tgtaaagtct ggcaatcgtc agggcgggca
     1381 gcgcaaaatt gttggtgaga aacaccaggg attttcccgt ttccggatcg ttgaacttta
     1441 cgcgccgcag cggacgatcg aagccttggc gcgtgtaaaa acccgtcaaa acaatcgttt
     1501 gatcgcagat caaccccgtc gttcgatcga cggggcgcga gtatcgacgc ttggccttca
     1561 tgttcgattt ggcgcgggtg acgaaaaaac tgccggcgcc gtcaagcttg gccagccgct
     1621 tgaaatcgac ataacctcga tccatcacgt agaaggctcc ggcctccggc gcgagatgat
     1681 cgaggatatt cacatcgtgc attttgccgt cgctgatgaa gataaacgac ggaatgttgc
     1741 cgcgcagatc gagcaacgta tgcagtttga tcgcggcttt ggttgtccga aacggagccc
     1801 acggaaaaac cgacaagcac aagtcgattg tcgaagaatc cagtgcgtaa acggtgtcga
     1861 tcccctcaag accgggcaag gtctcgtcgg cgtaaagctc gcgcgccatt cggataagat
     1921 gctgcgcgaa gtccgcgtaa atccgccaat ccctctcggc gttggcgttc gccattgtat
     1981 tgcgggagat ttgcgggcta cgaaagccca agcgataaag tttggcggac tgcgcccgca
     2041 agcacgcttc gatatcgcgc aaactctccc gccacgtcag ttgcgcgaag gcgaggcagc
     2101 ggaactggtc gagacaggag aaatgcttga tcttgtaatc gccgccatac cgcgccacgc
     2161 accgccgaaa cgtcgtcagc ggcgcatgat ccatcagttg cgcgaaaaca tattggccgt
     2221 gggacatgcg cgatccttgc aacggtgaaa atccgcaagg acctttccaa aaaaatcaaa
     2281 atcgagacca gccaacaaat ccttttccta aagttatatc agcatgttat cgccgcatcc
     2341 cacgtcaacg ctgggacacg ggtgattcat tacctcaaat aaataattag atataaagat
     2401 agggccgttc tacgaaacaa aaccaatctt caatgttctt ttaccaacgg ccgccttttc
     2461 gcgactttgc gatctgtcgg caaggcggcc taacctatcc cgcctgcaaa agccgcatgg
     2521 cgtcgtcgcg ctcgaagagg atgaggcagc gccgggcggc aaggccctgc tcggatttca
     2581 ggtggggatc gcgcaaaagc aggtagcggg cgaggtcgga cgcgcggtcc acgagcgacg
     2641 cggcctgcgg ccattgcgcc agacggaaac ccgggtcgcc gctctggcgc gggcccagaa
     2701 cttcaccccc gccacgcagg cggagatcct cctcggcgat cacaaccccg tcctcaacct
     2761 gccgcaggat gtcgaggcgc gactttgccg tctccgatag cggggccttg tagagcagaa
     2821 cacaggagga
//
LOCUS       DATMGX010000944         2348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_360503, whole genome shotgun sequence.
ACCESSION   DATMGX010000944 DATMGX010000000
VERSION     DATMGX010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360503"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VNR65_15425"
     CDS             <1..569
                     /locus_tag="VNR65_15425"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWJ20110.1"
                     /translation="TVGLPLPSVEVRIANPETGETLSPEDIGMIEVRGPNVCKGYWRM
                     PEKTKAEFRDDGFFITGDLGKIDGRGYVHIVGRGKDLIISGGFNVYPKEVESEIDALP
                     GVVESAVIGVPHPDFGEGVMAVVVSAKGSELQEKDVVEALKSRLANYKLPKRVFFVDD
                     LPRNTMGKVQKNLLRDAYTDLFCGKSKP"
     gene            696..920
                     /locus_tag="VNR65_15430"
     CDS             696..920
                     /locus_tag="VNR65_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20111.1"
                     /translation="MQSTLYNAQLWLGHAEQVRTLSKAVIDPHTKEQMLTVAAGFDRI
                     AHLAIQLKSTNGRSAQNERRLMSRPVRSAA"
     gene            1131..1346
                     /locus_tag="VNR65_15435"
     CDS             1131..1346
                     /locus_tag="VNR65_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWJ20112.1"
                     /translation="MSESVYKVIELIGTSTDSWEKAAAAAVERAGQSLRELRVAEVAQ
                     LDLVIEDGKVSAYRAKLKVSFKYEGKD"
     gene            complement(1649..>2348)
                     /locus_tag="VNR65_15440"
     CDS             complement(1649..>2348)
                     /locus_tag="VNR65_15440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWJ20113.1"
                     /translation="IARAHGLTEQQLLAANNLPDARSLRAGQSIAIPGQTAPRQAMAS
                     ATPQNRTAPRTGHASASAAPASDSEGTLQEAEQVKPSTEVKMTTTKPTEPTCEASLAN
                     PLPRMGKTFRKPVEGLVIGQFGPQRDGTVNEGLTISVPKGTPIKAAENGVVAYVGDEL
                     PGFGNLILIRHAEEYVTAYAHADEIMVKKCDVVKRGQTIATAGVTGDANQPQLHFEIR
                     KNAKPVDPSPLLGS"
BASE COUNT          476 a          671 c          733 g          468 t
ORIGIN      
        1 gcacggtcgg gctgcccttg ccgagcgtcg aggttcggat cgccaacccc gagacgggtg
       61 agacgctgtc gcccgaggac attggtatga tcgaggtgcg cggcccgaat gtgtgcaagg
      121 gctattggcg catgcccgag aagacgaaag ccgagtttcg cgacgacggc ttcttcataa
      181 ccggcgatct cggcaagatc gacgggcgcg gttacgtcca catcgtaggg cgcgggaagg
      241 atctcatcat ctccgggggc ttcaacgttt atcccaagga agtcgagagc gagatcgatg
      301 ccttgcccgg cgtggtcgaa agcgccgtca ttggggtgcc tcatcccgac ttcggcgaag
      361 gagtcatggc ggtcgtggtc tcggcgaagg gcagtgaact gcaagagaag gatgtggtcg
      421 aggcgctgaa gagccgcctc gcgaattaca agctgcccaa acgcgtattt tttgtggatg
      481 accttccccg caatacaatg gggaaggtgc aaaagaacct tctccgcgat gcctatactg
      541 atctcttttg cgggaagtcc aagccttaac cagtaggcct gcgcagcatt agcctgagga
      601 aacccgtgcc tgggcagcat cagtcaacac aattttcaca aaattcgatg taagggtgag
      661 ggctaggttg gttctagtcg cgagcaacaa tcgaaatgca gtctacctta tataatgcgc
      721 agctttggct cggccacgcc gagcaagtcc gtaccctcag caaggcggta atcgatcccc
      781 atacgaagga gcaaatgtta accgtcgcgg ccggtttcga tcgcatcgcc catcttgcga
      841 tccagttgaa gtcgacgaac ggcagaagcg ctcaaaacga gcggcgcctg atgagccgtc
      901 ccgtgcgttc cgcagcttga agagcgcgtc atcctccaga aacaccaccg gcgttgaggt
      961 gaattaaacc gcacctcgct gctggtgcgg cgccgccgaa gatttaccct tctcagcata
     1021 caacaccggg gccgcctgcc tcatttctag ccatgcgctc aagatttgct cggtttgact
     1081 tggcgactgc gacaaaaagt gccatccttg tatgtttgga ggatagtggc atgtcggaaa
     1141 gcgtgtacaa ggtcatcgag ctcatcggta ccagcactga ctcgtgggag aaggcggccg
     1201 ccgccgccgt tgagcgcgcc ggacagagct tgcgggaact tcgcgttgca gaggttgcgc
     1261 aactcgatct cgtgatcgaa gacggcaaag tgtcggccta ccgggcgaaa ctgaaggttt
     1321 ccttcaaata cgagggtaaa gactgactcc tggggcaggg cgtcagtgcg gagcgcgtaa
     1381 agtcggaatc ggttgcggcg caatcgaggc aatgcctcgt catcccggaa cgacgcctgc
     1441 gtcgtatccg ggatcgcaaa aaaggcgtct cgatctgtaa cggccccgga tatgcgccga
     1501 cgcgcattcc ggggcgacga ccatcgagac ggcggtgagc agccgccaac gctgcagccc
     1561 tctaacgaca aaagtcccgc gagagggcgg gacttcataa gcttttcacg gttgcgctgg
     1621 agggaacgcg cccctccatc tttcgatcct atgacccgag cagcggtgat gggtccacag
     1681 gcttcgcgtt cttccggatc tcgaagtgga gctgcggctg gttcgcatcc ccggtcacgc
     1741 cagcggtcgc tatagtctga ccgcgtttca ccacatcgca cttcttgacc atgatctcgt
     1801 ccgcatgggc gtaggctgtg acatactcct cggcgtggcg gatcaggatg aggttgccga
     1861 agcccggcag ttcatcgccg acataggcaa cgacgccatt ctccgcagcc ttgatgggcg
     1921 tccctttcgg aaccgagatc gtcaagccct cgttcacggt gccatcgcgt tgcgggccga
     1981 actggccaat aacaaggccc tcgacaggct tgcggaatgt cttgcccatg cgaggcagcg
     2041 gattggcgag tgaggcttcg caagtcggct cggtcggctt tgtcgtcgtc atcttgactt
     2101 cagtcgacgg ctttacctgc tcggcttcct gcagcgtccc ttcggagtcg gaggcaggag
     2161 cggccgaagc ggaagcatgg ccggtgcgcg gagctgtccg gttttgaggg gtggctgaag
     2221 ccatggcctg ccttggcgcc gtctgaccgg gaatcgcaat ggattgtccg gccctgagcg
     2281 agcgcgcatc gggcaggttg ttcgccgcaa gaagttgctg ctccgtcaag ccatgagcgc
     2341 gtgcgatg
//
LOCUS       DATMGX010000945         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_254343, whole genome shotgun sequence.
ACCESSION   DATMGX010000945 DATMGX010000000
VERSION     DATMGX010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254343"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VNR65_15445"
     CDS             complement(<1..496)
                     /locus_tag="VNR65_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWJ20114.1"
                     /translation="MPQRFWGVARAPLAAPSDHPLVNFGRIGVLLLNLGTPDGTSYWP
                     MRRYLKEFLTDRRVIEINPVVWWLILNTVVLTKRPFTSGEAYKSVWNSHRDESPLRTI
                     TRSQCEKIGAFLAREPRIEVDWAMRYGNPSIKSRLEALHNKGCDRILLFALYPQYSAA
                     TVATA"
     gene            complement(734..1468)
                     /locus_tag="VNR65_15450"
     CDS             complement(734..1468)
                     /locus_tag="VNR65_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20115.1"
                     /translation="MTQTVICIKWGTRYGPDYVNRLSSMVRRHTERPTRIVCYTDDKA
                     GITSEVETFPLPYIELPEYRRWFPWRKISLWQKELEGISGEVLYLDLDIVVTGGLDDF
                     FDHKPGHFCVARNWTQPHDRIGNTSVYRFTVGAHTYLYDQIMTDCEGVLSRYSNSQTF
                     ISREIKDMEFWPDSWCVSFKHSLIPAWPLNLFMTPRLPAGAKVVAFTGKPDPDEAMEG
                     RWPRGHWYHLFYKHVRPTPWIAEHWR"
BASE COUNT          457 a          553 c          587 g          440 t
ORIGIN      
        1 aggccgttgc aaccgtagcc gctgaatatt gagggtagag cgcaaaaaga agaatgcggt
       61 cgcagccctt gttgtgaagc gcttcaagcc gcgacttaat gctgggattg ccgtatcgca
      121 ttgcccagtc gacctcgatc cggggctccc tcgccagaaa ggcgccgatt ttttcacatt
      181 ggctgcgtgt gatcgtgcgg agcggcgact cgtcgcggtg cgagttccag accgatttgt
      241 aagcctcgcc cgaggtgaag gggcgcttcg taagaacgac cgtattgaga atcagccacc
      301 agacgaccgg attaatttca atgacgcggc ggtcggtcag gaactccttc aagtagcggc
      361 gcatcggcca atagcttgtg ccgtcgggcg tgccgagatt gagaagcaac acgccgatgc
      421 ggccgaaatt gaccaaggga tgatctgaag gcgcagcaag cggcgcgcgc gccactcccc
      481 aaaaacgctg cggcataaaa ttcatcccga tgcctcccat tcagccgggc gtttaccgtc
      541 gactttagtt aacacggata gcgttgtacg cgccttgtgt catcctgaca acgcaccgaa
      601 aagctcaaag gatcatcatt ttgcgctgga gccgtgatca gaaatccatg ctgcgccaaa
      661 ataatgactt ttgcctcaat gcgcaaacga aggcttgacc ataagccgcc cattcccgga
      721 aggcccgcgt cgatcaacgc cagtgctcgg cgatccaggg cgtcggacgg acatgcttat
      781 agaagaggtg ataccaatgc ccccgcggcc agcggccctc catcgcctcg tcggggtcgg
      841 gcttgcccgt gaaggccacg acttttgccc cggctggcag ccgcggagtc atgaaaagat
      901 tgagaggcca cgctggaatg aggctgtgct tgaaactcac acaccaggag tcgggccaga
      961 actccatgtc cttgatctcg cgcgagatga aggtctggct gttgctatag cgcgaaagaa
     1021 cgccctcgca atccgtcatg atctggtcgt aaagataggt gtgggcgccg accgtgaagc
     1081 gatagacgga tgtgttccca atccggtcat gaggctgtgt ccagttgcga gccacgcaga
     1141 agtgaccggg cttgtgatca aaaaaatcgt ccaggcctcc ggtgacaaca atgtcgagat
     1201 cgagatagag gacttcgcct gagattccct ccagctcttt ctgccagagc gaaatcttcc
     1261 gccatgggaa ccagcgtcgg tattccggca actcgatata aggaagtgga aaggtttcaa
     1321 cctcgcttgt gatgcctgcc ttatcgtcgg tgtaacagac aatccgcgtt ggccgttcgg
     1381 tgtgtcgtct caccatggag gagagccggt taacatagtc tggcccatat cgagtgcccc
     1441 actttatgca aatgacggtc tgtgtcatcg ttcgctcaac tccgccgatt tcacggaaag
     1501 gcaattggcg cggttttttt aagcgccggt ctcaagcgtg acgttgatcc ctgacgatca
     1561 tggcaatttg gcgaacatga ttaccaggtg agctggtatc ccagatacca ggaaaactaa
     1621 ttgatatcgt tcgccttctg aaggccccct ccggtgaaac aagcaaatgg ttgtgctcga
     1681 cgaaatcgta atggttgttt ccgcttcgcg acgcggaact gccttaaact ccagcgagac
     1741 tgtcgaaagc gcgagcgtgt gcaatggtcc ggcgtggtct agagccgcgt tggtgcgcac
     1801 gtggcgggct gttcgaccac agatactttt ccgcctttaa tcggttccga tcagaggcgg
     1861 gttgatctgg agaggcgttc accaacttgg gaaatgcgat ccactcatga agatttgggg
     1921 accttatagc tttacaggcg ccgcgttggc tgtcgcgact gtgctttgcg atcagcttca
     1981 caaagcgtgg atgatcacga tcttcggcga cgctcagagg ccgaagatca cggtcac
//
LOCUS       DATMGX010000946         5106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_287413, whole genome shotgun sequence.
ACCESSION   DATMGX010000946 DATMGX010000000
VERSION     DATMGX010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287413"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VNR65_15455"
     CDS             complement(<1..297)
                     /locus_tag="VNR65_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20116.1"
                     /translation="MTALDRQSSQAASGEVVYDDAGRTAAAENTADKSKAEVSTPVGG
                     AGTGCHAGAAEMKKAAAAAGKSEVLEQYAKDYPQGPHDQPQSMCPAFGSLRVGLR"
     gene            complement(312..1322)
                     /locus_tag="VNR65_15460"
     CDS             complement(312..1322)
                     /locus_tag="VNR65_15460"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase iron protein subunit
                     X"
                     /protein_id="HWJ20117.1"
                     /translation="MTLDVDQATALEQQAAALRAEAAIEPDAVTATEVKKETQIIAIY
                     GKGGIGKSFTLANLSYMMAQQGKRVLLIGCDPKSDTTSLLFGGRATPTIIETSSKKKA
                     AGEEVLIGDVCFKRDGVFAMELGGPEVGRGCGGRGIIHGFELLEKLGFHEWGFDYVLL
                     DFLGDVVCGGFGLPIARDMCQKVIVVGSNDLQSLYVANNVCSAVEYFRKLGGNVGVAG
                     MVINKDDGTGEAAAFCSEVGIPVLSAIPQHDDIRKKSANYEIIGLPGGQWAPVFETLA
                     KNVAEAPPVRPTPLSQDGLLGLFKGDAVGRGVVLDPASQSDMRGHEIVEKPSLEIVYD
                     VA"
     gene            complement(1319..2260)
                     /gene="bchC"
                     /locus_tag="VNR65_15465"
     CDS             complement(1319..2260)
                     /gene="bchC"
                     /locus_tag="VNR65_15465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthesis pathway protein BchC"
                     /protein_id="HWJ20118.1"
                     /translation="METLAVVLQEPQQLGLQRLQLPTPGDADLVVDVEWSGISTGTER
                     LLWSGRMPDFPGMGYPLVPGYESVGRVSWAGSSTKIRVGERVFVPGANCYGAIRGLFG
                     GTASRVVLPESRTIPIAEELGEKGILFALGATAYHAAFSDKGVAADLIVGHGVLGRLI
                     ARIVIASGAPAPMVWEKNTSRFSGAVGYQVIDPDQDPRRDYRVVCDVSGDSSILDALV
                     SRLAPGGEIILAGFYADRLSFSFPPAFMKEARIRIAAQWQKADMLAVKQLVDSGRLSF
                     DGLITHRSTADGAPSAYRVAFEDPACLKMVLDWRKCV"
     gene            complement(2372..3556)
                     /locus_tag="VNR65_15470"
     CDS             complement(2372..3556)
                     /locus_tag="VNR65_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWJ20119.1"
                     /translation="MTPTVTPAQNIVKVDKPNLLNGLMDRFFAFRDRCLTSPRFIRFA
                     ETFPPTRIIARREASALFDLCSGFVYSQVLLACVRLRLFDALKPGPLSVEEIANRLNL
                     SADATGRLLTAAVGLRLVERRSGGRYGLGGLGAAMLTSPGLVTMVEHHALLYRDLDDP
                     VRLLRDENHVPGQDGFGKATNLASYWPYAEGDTGANRDAVSDYTGLMAATQPAIAQEI
                     LDAYDFKPHRRLMDVGGGNGAFLLCAARRFPHLQLVLFDVPAVAELAAARFTEAGIAD
                     RATVAGGDFLKDLPQDADVISLIRVLLDHDDETALGILKSARAALPQGGVLLVAEPMA
                     ETAGAEKVAAYFSLYLMAMGKGRPRTEQKLQVLLREAGFQRIQRHSTCQPLLVRLLVA
                     RV"
     gene            3636..>5106
                     /gene="crtI"
                     /locus_tag="VNR65_15475"
     CDS             3636..>5106
                     /gene="crtI"
                     /locus_tag="VNR65_15475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HWJ20120.1"
                     /translation="MHEKRIVVVGAGIGGLAAAVQLAAEGCGVTVVEKFDGPGGKMRQ
                     VQTGGVSIDAGPTVLTMREVFDGLFEKAGASIGDYLTLKPAGVLARHAWEAGGELDLF
                     ADVTQTVDAIGAFAGADNARGYLSFCSEAANIYRILDRSFIRVQKPNLPALIWRVGPL
                     KFGDLWRINPYQSFWSAVCRHMSDPRLRQLFARYSTYCGASPFFAPATLMLVANVEQR
                     GVWLVEGGMVRIAAALESLAQRLGVVFRYRTEVTEVLATKGRATGIKLEGGEQLNADA
                     VVLNADASAASGGLLGPAVEKAARHVPARSRSLSALTWAFQAEARGFPLARHTVFFPN
                     ASYASEFDAIFRQRKLPPYPAVYICAQDRDDRGGKHAEGAERFLAIVNAPAMLDEKAL
                     SRSEIEECETKTFRLMAACGLTLQPTEGAMIRRTPQDFERLFPGTGGAIYGRVSHGWM
                     ASFQRQGIRSRLPGLYFAGGSVHPGPGIPMAALSGQLASE"
BASE COUNT         1047 a         1560 c         1505 g          994 t
ORIGIN      
        1 acgcagaccc acgcgtagcg agccgaaggc gggacacatg ctctgcggct gatcgtgcgg
       61 cccttgagga taatccttcg cgtactgttc gagtacctcg ctcttgcccg cggcggcggc
      121 ggcctttttc atttcggccg cgcctgcgtg acacccagtg cctgctccac caaccggggt
      181 gctcacctcg gccttcgact tgtcagccgt attctcggca gccgcagtac gtccggcgtc
      241 gtcatagacg acttcaccgg atgcggcttg agaggattgg cgatcaagtg ctgtcataaa
      301 ctcactgctc cttacgcgac gtcgtagacg atttcgaggg acggcttctc gacgatctca
      361 tggccacgca tgtcggactg gctcgctgga tcaaggacaa caccacgccc aaccgcgtca
      421 cccttgaaga ggcccagcag gccatcctga gaaaggggcg tcggccgcac aggcggcgcc
      481 tccgcaacat tcttagcgag ggtctcaaac acaggcgccc actgcccgcc tggtaggccg
      541 atgatttcgt aattcgcgct tttcttgcgg atgtcgtcgt gttgcgggat ggcggagagc
      601 acaggaatac cgacctcgga acagaaggcg gccgcctcgc ctgtcccgtc atccttgttg
      661 atgaccatgc ctgcaacgcc gacattgcca ccgagcttgc ggaaatattc gaccgcagag
      721 cagacgttat tggcgacgta gagcgattga aggtcgttcg aaccgacgac gatcaccttt
      781 tggcacatgt cgcgggcaat cggcaggccg aaaccgccgc acaccacgtc gcctaggaaa
      841 tcaagcagga cgtagtcgaa accccattca tggaagccaa gcttttcaag aagctcaaag
      901 ccatggatga tgccgcgtcc gccgcagccg cggcccactt caggtccgcc gagttccatg
      961 gcgaagacgc catcgcgctt aaagcagacg tccccaatca gaacttcctc gcccgctgcc
     1021 ttcttcttgg acgacgtctc gatgatcgtc ggggtggcgc gaccgccaaa cagaagggac
     1081 gtggtatcgc tcttgggatc gcagccgata agcaggacgc gcttgccctg ctgagccatc
     1141 atgtaagaca ggttcgcgag cgtgaagctc ttgccgatcc cgcccttgcc gtagatcgcg
     1201 atgatctgcg tttctttttt gacttcggtc gcagtaaccg cgtcaggctc gatagcagcc
     1261 tccgcgcgga gcgcagccgc ttgctgctcg agggcagtcg cttgatctac atccaaagtc
     1321 atacgcactt tctccaatct agaaccatct tcaggcaggc cggatcttcg aaggcgacgc
     1381 ggtaggcgct tggggcgccg tctgctgtac tgcgatgcgt aatgagacca tcaaaggaca
     1441 agcggccact gtcgaccaat tgcttgacgg cgagcatgtc cgccttttgc cactgcgcgg
     1501 ctatccgtat ccgcgcttct ttcatgaagg cgggcgggaa gctgaaagag agtcgatccg
     1561 cgtaaaatcc ggcgagtata atctcgccgc caggggcaag ccgcgacacg agcgcatcaa
     1621 gaattgatga gtctccactt acatcgcaaa ctacgcgata atcccggcga ggatcttgat
     1681 ccggatcaat aacctggtag cccacggcac cggaaaaccg gctcgtattc ttttcccaca
     1741 ccattggcgc gggcgcacct gacgcaatca cgatgcgcgc aatcaagcga ccgagaacgc
     1801 catgcccaac aataagatct gccgccacgc ccttatctga gaaagctgcg tggtaagccg
     1861 tcgcgcctag cgcaaacagg ataccttttt caccaagttc ttcggcgatg ggaattgtcc
     1921 ggctctcggg caaaacaacg cgcgacgctg tgccaccgaa gagaccgcgg atcgcgccgt
     1981 agcaatttgc gcccggcaca aaaacacgtt cgcccacgcg aattttggta gacgaaccgg
     2041 cccaggaaac acgacccaca gattcgtagc ctggaacaag aggatagccc atgcctggaa
     2101 aatcaggcat gcgccccgac cacaatagcc gctcagtccc ggtgctgatc ccgctccatt
     2161 ccacatcgac gacgaggtcg gcatcgccgg gagtcggcag ctggagccgc tgcagcccga
     2221 gttgttgggg ctcctgcaac actaccgcta aggtctccat tgggaactct ggaaggcggg
     2281 tcatgcagac tgatatctct tcacaagttc gtgtgtcatc ctagctttac cagcttatat
     2341 gtcaatccaa acttacactg attgcaagcg cttaaacccg agccacaagc agacggacaa
     2401 gcaggggctg acatgtcgag tgcctttgga tgcgctgaaa cccggcctcc cgcaaaagca
     2461 cctgcagttt ctgctccgtg cgcggacggc ctttgcccat ggccattaaa taaaggctga
     2521 aataggcagc aactttctcc gcgccggcgg tctctgccat gggctccgcc acgagaagca
     2581 cgcctccctg cggcaatgca gcgcgcgccg acttgaggat acccagcgcg gtttcgtcgt
     2641 catggtcgag aagaacgcga ataagcgaaa tgacatctgc atcttggggc aggtccttga
     2701 ggaagtcccc gcccgcgacc gtcgccctgt cggctatccc ggcttctgtg aacctggctg
     2761 ctgccagctc cgcgaccgcg gggacgtcga acaggacaag ttgcaggtgg gggaagcggc
     2821 gggcggcgca taagagaaac gcgccattac cgccgccgac atccatcagg cggcgatgcg
     2881 gtttgaaatc gtatgcgtcc aaaatctcct gcgcgatggc cggctgcgtc gcggccatga
     2941 gccctgtgta atccgagaca gcatcccggt tcgcgccggt atcgccttcg gcgtaaggcc
     3001 aataggacgc aagatttgtc gccttgccga agccgtcctg ccccggcaca tggttctcgt
     3061 cgcgtaaaag gcggacagga tcgtcgaggt cgcgatagag aagcgcgtga tgctccacca
     3121 tcgtgacaag gccggggctt gtgagcatcg ccgcgccaag gcctccgaga ccatagcggc
     3181 cgccgcttcg ccgctccaca agacgcaaac caaccgcggc cgtcaggagc cgccccgtcg
     3241 catcggcgct caagttcagc cggttcgcga tctcttccac ggaaagcggc ccaggcttca
     3301 aggcatcgaa cagcctcaat cgcacgcagg cgagaagcac ctgggaatag acgaacccag
     3361 aacacaaatc aaaaagtgca ctcgcttcgc ggcgcgcgat gatgcgcgtt ggcggaaagg
     3421 tttcagcaaa ccgtataaaa cgcgggcttg tcagacagcg gtcacgaaag gcaaaaaacc
     3481 ggtccatcaa gccgttcagg aggttgggct tatccacttt cacgatattt tgggctggcg
     3541 tcactgtggg tgtcatctat agttgtcact tggactgtca tcctaaatgg acagtcctcg
     3601 cgcggcatgt caacgccgtt ttgggagaac atcgcttgca cgaaaagcgc attgtagtgg
     3661 tcggcgccgg gattggaggc ttggcggccg cggtccagct tgctgcggag ggctgcggcg
     3721 taaccgtcgt cgagaaattc gacggacccg gcggcaagat gcgccaggtg caaactggcg
     3781 gtgtgtccat cgatgctggt cccactgttc tgacaatgcg cgaggtgttc gacggccttt
     3841 tcgaaaaggc gggcgcgtcg ataggggatt accttacgct caagccggcg ggtgttctgg
     3901 cgcgtcacgc ctgggaagca ggcggcgaac tcgatctttt cgccgatgtc acacagaccg
     3961 tagacgccat cggggccttt gcaggcgcgg acaacgcccg gggttatctc tctttttgca
     4021 gcgaggccgc aaatatctac cgaattctgg atcgctcctt cattcgcgtc cagaaaccta
     4081 accttcccgc tctcatttgg cgcgtcggac cgctgaaatt cggcgatctc tggcgcatca
     4141 atccctatca aagcttctgg agtgcggttt gccgccatat gagcgatccg cggctgagac
     4201 agcttttcgc gcgttattcc acttattgcg gcgcttcgcc gtttttcgcg ccggccactc
     4261 tcatgctcgt cgcgaatgtc gaacagcgcg gcgtctggct tgttgaaggc ggcatggttc
     4321 gcatcgccgc tgcgctcgag agcttggcgc aacgactagg cgtcgttttc cgttatcgga
     4381 cggaggtcac cgaagtgctt gccacgaaag gcagagcaac cggaatcaaa ctcgaaggtg
     4441 gcgaacagct gaatgccgat gccgttgtcc tcaatgccga tgcctctgcc gcttccggtg
     4501 ggctgctcgg ccctgccgtt gagaaagcag cccggcacgt tcctgcccgc agtcgctcat
     4561 tgtccgccct cacctgggcg ttccaggccg aggcgcgcgg ctttcctctg gcgcggcata
     4621 cagtcttctt tccaaatgcc tcctacgcgt cggagttcga cgctatcttc aggcaaagga
     4681 aacttccgcc ctatccggcg gtctatatct gtgcgcagga tcgggacgat cgcggaggaa
     4741 aacatgccga gggagccgag cggttcctcg ccatcgtcaa tgccccggcc atgcttgatg
     4801 agaaagcgct cagtcgatcg gagatagaag aatgcgagac aaaaaccttc cggctgatgg
     4861 cggcatgtgg tctgacgctc cagccaacgg aaggagccat gatcaggagg acgccgcagg
     4921 atttcgagag gctgtttccg ggcacggggg gagcgatcta cgggagagtc tcccacggat
     4981 ggatggcgtc ctttcagcgg caggggatcc ggtcgcggct gccgggtctt tatttcgcgg
     5041 gggggagcgt gcatccgggg ccgggcatcc caatggcggc gctttcgggc cagctcgcat
     5101 cggaag
//
LOCUS       DATMGX010000947         2028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_208210, whole genome shotgun sequence.
ACCESSION   DATMGX010000947 DATMGX010000000
VERSION     DATMGX010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208210"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..1608)
                     /locus_tag="VNR65_15480"
                     /pseudo
     CDS             complement(340..1608)
                     /locus_tag="VNR65_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023098450.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            1755..>2028
                     /locus_tag="VNR65_15485"
     CDS             1755..>2028
                     /locus_tag="VNR65_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20121.1"
                     /translation="MMEEKKLTGRIAREKIKLMQVPPPPQGAVDAFLALGDPTGIVAD
                     AMDELGIPCGVVGAHVLKPTIPGTVMVGPALTIRNVAQRADALDGAR"
BASE COUNT          365 a          594 c          620 g          449 t
ORIGIN      
        1 gacgcggcaa agaagaacgg ctggccggtg atcagtatga agaacgactg gaagcacatc
       61 ttcgccttcg acgagtaacg ccgctctgac tgctatttcg agctactggg caattattgt
      121 ggccaggcta accacttttg cacggctaat gtcgcttatg ggtcttggtc gtgtgaaaac
      181 gcctgacaga agcatagacg cggcgcgaac tcggctaggt agccatgcgc ggttattatt
      241 tttggatttg acggcttttg cgactgtagg cgcggctgat gcggattgca gcggtcgtgg
      301 gcggttctac aatggcaggc gggcgcatgt cggcggcggc tatgccctca tcgccgccat
      361 gagcggtttg gtgcccatga tgttcatgac gcgcgtgagg ttataggcca gcacgtgcag
      421 cgccatctcg gtggcaaccc ggggcagcgt cttcatcagg aaatgggttg ctcccatccg
      481 ggccttgatc gttccaaagg gatgctcgac cgtctcgcgc cgcagacgca tcttttccgg
      541 atgttcgtcg agccggcgct gcaccgcctc aagaacgtgc tcatgctccc atcgggtgat
      601 ccgtcgttcc ttgccagtgg tgcagctgtt cttgatgccg cagctctggc aggctttggt
      661 cgagtagcgg cgcaagacca atccattttc cgaggtggtg aaggagtagg cgagcctttc
      721 accggcagga cagatgtaaa cgtcctcttc agccacatat cggaagtcct gcttgccaaa
      781 gcgcccttcc gcctttgagt tcgaggtcat cggtttgggc agcgtgaccg tgatgccagc
      841 attctcgcat gccaggatct cctcgctgtt gaagtagccg cggtcggcga ctgcatcgag
      901 gctctccact tccagcgtcg ccttcgcctc tttagccaca ggtgagagct gcgaccggtc
      961 ggtgccgacg ttggtcacct catgcgtgac gatcaaatga tgtgtggtat cgacggcaac
     1021 ctggacgttg tagcccacga cgccagagcc gcggccgctc gtcgccatcg agcgggcatc
     1081 gggatcggtc agcgatatct gctggtccgg cgtggcgagc attttcatct tgaaaatctc
     1141 gagccgctgc atctgctcct tcagcttcgc gatcttctct ttcagacggt tcgtcttggt
     1201 tttgagtgcc tcggacggct cctgcctatc cgcactgtcg agttgctgca gatagcgcgc
     1261 gacgctctcc tcgatctgcg ccatgcgccg ctccatcttg gcgcgtgtga agttcctgtc
     1321 acggttgttc accgccctga acttgctgcc atcgatcgcc acgctggctt gcgtgagcag
     1381 gcccagggta cgacagagcg agacaaaccg ggcgcagacc tgccggaggg cgcggccgct
     1441 gtccttgcgg aagtcggcaa tcgtcttgtg gtcgggagca agccggcccg tcagccacat
     1501 cacctcaacg ttgcgtccgg cttcacgctc caaccggcgg ctcgactgca cccggttgag
     1561 atagccgtag atatagagct tcagaaggac cgacgggtga tacgaaggcc atgaattgag
     1621 gcaagacgta taggcggtca ttcaaggtgg tgatagctgg tattccccgc cgaaatttga
     1681 cacgcttttg acgctggctc cccaatgcat cacactcatt gttgggccta tgtccacttc
     1741 ccgacaggtg caagatgatg gaagagaaaa agctcactgg tcggatcgct cgcgagaaga
     1801 tcaaactcat gcaggttccg ccgccgccgc aaggcgccgt cgacgcgttt ttggcgctgg
     1861 gcgatccgac cggcatcgtg gcggatgcga tggatgaatt ggggattccc tgcggcgtcg
     1921 tgggcgccca tgtgctgaag cccacgattc cgggtacggt gatggttggt cctgcgctca
     1981 ccatccgcaa tgtcgcccaa cgcgccgacg cactcgatgg cgcccgcc
//
LOCUS       DATMGX010000948         4863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_219125, whole genome shotgun sequence.
ACCESSION   DATMGX010000948 DATMGX010000000
VERSION     DATMGX010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219125"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..886
                     /locus_tag="VNR65_15490"
     CDS             23..886
                     /locus_tag="VNR65_15490"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HWJ20122.1"
                     /translation="MQPVSQHSPTGHEFPFTAADFNTIVGIVYERSGIVLAAHKRDMT
                     YSRLARRLRALGLRSFSDYCSLLEGKNGSNEIGFLINAITTNLTKFFREPHHFDHLRE
                     HVLRPALNSSLKYKPRIRVWSAGCSSGEEPYSVAMTALMLQKESGCDWDFRILATDLD
                     TSMLKKAKAGVYSRACIESVPAGMRERYFDRLAGEPENVVLAKVAQEQVSFRQLNLLG
                     PWPMSGKFDVIFCRNVMIYFDAKTKTTLIDRFHGQLADGGWLYVGHSESLLDHQSRFK
                     LRGRTIYQKIS"
     gene            886..1518
                     /locus_tag="VNR65_15495"
     CDS             886..1518
                     /locus_tag="VNR65_15495"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20123.1"
                     /translation="MTAHALHRKKNEANTGKAGIYLAEFGAHAVRVLPGETYTTSRKD
                     EAIVTVLGSCVSACIRNPRTGIGGMNHFMLPESTTGQWGEELSAAMRYGNFAMEALIN
                     AILKSGCARSDMEIKLFGGANFSAGVAMIGQKNSDFALRYLKEEGLRVVASDLGGVLG
                     RRIHYHPATGKVARLFLRGSGKDQVAAEERRYGAELRRKPVDGGDVQLFD"
     gene            1528..2601
                     /locus_tag="VNR65_15500"
     CDS             1528..2601
                     /locus_tag="VNR65_15500"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HWJ20124.1"
                     /translation="MGGSNPIRVLVVDDSALMRELIRGILSSDPGIEVVGAVCDPLAA
                     RQKIKELNPDVLTLDIEMPRMDGITFLDKIMTLRPMPVVMVSSLTQEGAEATLRALEV
                     GAVDFVAKPAINLQAGLEEKRAEIIAKVKAAATARVRRRAAPQGERKTPLALAANYSS
                     TEKIVTIGASTGGVEAVNQILQSMPPDAPAILVTLHMPENFTASFAKRLNAACAIQVA
                     EASDGERVLPGHAYIAPGSTHLRLGRSGANYVCIVRGKERVSGHCPSVDVLFHSTADA
                     AGKNAIGVILTGMGKDGADGLLAMRHNGASTLGQDEESCVVYGMPKVAFERGAVEAEV
                     SLGRMAQEILGRCTSGGARNIRI"
     gene            2653..3774
                     /locus_tag="VNR65_15505"
     CDS             2653..3774
                     /locus_tag="VNR65_15505"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWJ20125.1"
                     /translation="MNLALKKASSGEHEWSSASSSHGLGASPAAKQIAQELISFATAQ
                     SKATEALRGELSHTSQDVERSVHGLVDKFQAMATTARDQTETVQSLMSSVQTIEINGE
                     SVPLSELARSIGDVVSLLVEKIIHLSSRSVAMVYALDDVQVEIKAMQASVAQIDKINK
                     QTNLLSLNAKIEAARAGSAGRGFAVVASEVGELARAVNTLSDTVKRQMACVSDGVRRG
                     DNLLREISAIDMSEENLKAQDRIKFMIDRLLSQNSTIAEALRKTASTSQQMEQAVSGA
                     IMDMQFQDRVMQHIQNVNGGLAVMSRTGGLLAARSLAELEVLPLASSETALLQEISDQ
                     FTLSELRERFIAALQLAGVLGLKDAANAKPAANDDVDLF"
     gene            3785..4090
                     /locus_tag="VNR65_15510"
     CDS             3785..4090
                     /locus_tag="VNR65_15510"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWJ20126.1"
                     /translation="MRLETYKSNNKFEAEISDKLTSSDLSNFRELLSAIKQSTCKVIV
                     LDLSNLDWIDSAGLGMLILAKELAEKEELELVLRSPRGHVKSLLELGRFEKIFSIEA"
     gene            complement(4408..>4863)
                     /locus_tag="VNR65_15515"
     CDS             complement(4408..>4863)
                     /locus_tag="VNR65_15515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWJ20127.1"
                     /translation="MISPLSWMPIAILAFSTWEGAIVFLITVAAVWPVLFSTAAGVRR
                     IDPAWFKVARNLGANPFHLLTAVILPAIAQDILTGIRLALGVAWIVLVPAEYLGVKSG
                     LGYAINDARDTLEYDRLAAVVLIVGLIGFALDAGLQRLIQRLSWLRQET"
BASE COUNT         1102 a         1320 c         1439 g         1002 t
ORIGIN      
        1 tcagtaacag agtgcgttga gtatgcaacc cgtaagccaa cactctccga ccggtcatga
       61 gtttccgttc accgctgcgg atttcaacac gattgtcgga atcgtttatg agcgaagcgg
      121 catcgttctt gccgcacata agcgggacat gacctactcc cggctcgcaa ggcgcctccg
      181 cgccttgggg ctgaggtcgt tcagcgatta ctgctcgctc ttggaaggca agaatggcag
      241 caacgagatt ggctttctca tcaatgcgat cacgaccaat ctgacgaagt tctttcgtga
      301 gccgcatcat ttcgaccatc ttcgcgagca tgttcttcgc cctgcgctca attcgtcgct
      361 caagtacaag ccgaggataa gggtctggtc cgcgggatgt tcttctggcg aagagcctta
      421 ctccgtcgcc atgacggctt tgatgcttca aaaagaaagc ggttgtgact gggattttcg
      481 gatccttgcc acggatctcg acacatcgat gctgaagaag gccaaggcag gcgtttattc
      541 gcgcgcttgt atagagagcg tgcccgctgg tatgcgcgag cgttacttcg acagactggc
      601 aggggagccc gagaatgtcg tgctggcaaa ggtggcccag gaacaggtca gtttccggca
      661 gcttaatctg cttggccctt ggccgatgag cggtaagttc gatgtgatct tctgcaggaa
      721 cgtcatgatt tacttcgacg ccaagacgaa gaccacactc atcgacagat ttcatgggca
      781 gcttgccgat ggcggatggc tttatgtcgg ccactccgaa tcgctcctgg atcatcaaag
      841 ccgcttcaag cttcgtggcc gcacgatcta tcagaagata agctgatgac cgcgcacgca
      901 ttacatagga aaaagaacga agcgaatacg gggaaagccg gaatttatct cgcagagttc
      961 ggcgctcacg cagttcgtgt tctgcctggc gaaacctata cgacctctcg caaagatgag
     1021 gccattgtca cggtgctcgg ctcttgcgtc tcagcctgca tccgcaatcc gaggaccggc
     1081 atcggcggaa tgaaccactt catgctgccg gagagcacga cggggcagtg gggcgaagaa
     1141 ttgagcgcgg cgatgcgcta cggaaacttc gcaatggaag ccctgatcaa cgccatcctg
     1201 aagtccggct gcgcaaggtc cgatatggag atcaagctct ttggtggcgc aaatttttcg
     1261 gcgggggtgg caatgatcgg tcagaaaaac agcgactttg ccctgcgcta cctgaaagag
     1321 gaaggcttgc gtgttgtcgc atccgacctc ggcggtgtcc tcggccggcg catccattat
     1381 cacccagcta ccggcaaggt tgcgcggcta tttctaagag ggtcgggcaa ggatcaggtt
     1441 gccgcggagg agcgccgcta tggtgcggag ctacggagaa agcctgtcga tggcggtgat
     1501 gttcagcttt tcgattgagg taggctaatg ggagggtcca atccaatccg ggtcctcgtc
     1561 gtcgatgact ccgccctcat gcgagagttg atccgcggaa tattgtcgtc agatccgggt
     1621 attgaagtgg tgggcgcggt ctgtgatccg cttgccgcgc ggcaaaaaat caaggaactc
     1681 aatccggacg tcctgacgct ggatatcgag atgccgcgaa tggatggcat caccttcctt
     1741 gacaagatca tgacgcttcg gccgatgccg gtcgtcatgg tttcgtcact gacgcaggaa
     1801 ggcgcggaag caactctaag ggcacttgaa gttggcgctg tcgatttcgt ggccaagccg
     1861 gcaatcaatc tgcaagccgg attggaagag aagcgggccg agatcatcgc aaaggtcaaa
     1921 gcggccgcaa ctgcacgtgt gcgtcggcgg gcggcgccgc aaggcgagcg caaaaccccc
     1981 ttggccctgg cggcgaatta ctcctccacc gagaaaattg tcacgatcgg agcctccacg
     2041 ggaggcgtgg aggccgtcaa tcagatcctt cagtcgatgc cgccggatgc tcccgctatc
     2101 ctcgtcactc tgcatatgcc ggaaaatttc accgcaagtt tcgcaaagcg cctgaatgcc
     2161 gcatgcgcca ttcaggtcgc tgaagccagc gatggcgaac gcgttttgcc gggccatgcc
     2221 tatattgcgc ccgggagcac ccacctccgc cttggccgtt cgggggcgaa ctacgtttgc
     2281 atcgttcgcg gcaaggaacg ggtcagcggc cattgcccct cggtcgatgt gctgtttcac
     2341 tcgactgcgg acgctgccgg aaaaaatgcc atcggcgtca ttctgaccgg aatgggcaag
     2401 gatggagccg acggcctgct ggccatgagg cacaatggcg ccagtacgct cggtcaagat
     2461 gaggagagtt gtgtcgtgta tggaatgccg aaggtggcct tcgagcgtgg agctgtcgaa
     2521 gctgaggttt cactcggccg aatggctcag gaaatcctcg gccgttgtac ctctggcggc
     2581 gcgcgcaata ttcgcatcta aggccaggac agacctcctg gccctcttta tcaggcaaca
     2641 aaagatcgag ccatgaatct agcgcttaaa aaggccagct caggcgagca cgaatggagt
     2701 tccgcctcga gctctcatgg cttaggagcc tcgcccgccg cgaaacagat cgcacaggaa
     2761 ctgatcagtt tcgcgacggc gcaaagcaag gctaccgaag cgctgagggg cgaactttct
     2821 cacacctccc aggatgtcga aaggagcgtg cacggtcttg tagacaagtt ccaggccatg
     2881 gcgaccacgg cccgggatca gaccgaaacc gtccagtcgc tcatgtcatc cgtgcagacg
     2941 atcgagataa atggcgagtc tgtgccgcta tcggaactcg ctcgaagtat tggagacgtt
     3001 gtgtcgctgc tcgtcgagaa gatcatccat ctctcgtctc gaagcgtcgc catggtttac
     3061 gctctcgatg acgtgcaggt tgagatcaag gccatgcagg cgtccgtcgc tcagatcgac
     3121 aagatcaaca agcagacaaa tctgctttca ctcaacgcga aaatcgaggc ggcgcgcgcc
     3181 ggctcggcgg gccggggctt cgccgttgtc gcgagcgaag tgggcgaact ggctcgcgca
     3241 gtcaataccc tatcggacac ggtcaagcgt cagatggctt gcgtatcgga tggggtacgg
     3301 cggggcgata atttgcttcg tgagatctca gccatcgata tgtccgagga aaatttgaag
     3361 gctcaagatc gcatcaaatt catgatcgac cgtcttctga gccagaattc gacgattgca
     3421 gaggcgttgc gcaagacagc ctcgacgtca cagcaaatgg agcaagccgt ttcgggcgcc
     3481 attatggaca tgcagtttca agacagggtg atgcaacata ttcagaatgt gaatggagga
     3541 cttgccgtca tgagccgcac aggcggcctg cttgcggcga ggtcactcgc tgaacttgaa
     3601 gtcttgcctt tagcgtcatc ggaaaccgcc ctgcttcagg agatctccga ccagttcacg
     3661 ctcagtgaac ttcgcgagcg gtttattgca gccttgcagc ttgcgggggt actcggtctc
     3721 aaagatgctg caaacgcgaa gccggcagca aacgacgacg tagacctatt ttgatggagg
     3781 tggaatgcga ctggagacat acaaatcaaa taacaagttt gaagctgaga tcagcgacaa
     3841 attaacgtca tcagatctct ctaactttcg tgagctgcta tctgcaataa agcaaagcac
     3901 atgtaaagtt atcgtgctcg atctcagcaa tctcgattgg atcgactcgg caggcttggg
     3961 aatgcttata ctggcgaagg aactcgcgga aaaggaggag ctggagcttg ttcttcgttc
     4021 gcccagaggg cacgtgaagt cgcttctgga gcttggccgt ttcgagaaga tcttcagtat
     4081 tgaggcctaa acaaacttcg ctgatctggg ccagcggatt gtcatcccgg ccaagcgaag
     4141 cgcgagccag gatcgcaaaa aggtaagcgc tttcatctgt tacgatcccg ggtaaggctt
     4201 cgcctttccg ggacttgatt actcgcatga atccgctagc agaagaagcc atgaaaacat
     4261 aggccaattt cgctccactg tcttggcggc ttgacccggc catccagaaa aacacgggtc
     4321 aggcccggcc atgacaggtt agcgaaaatt cgccaatgtt tttaaggctt cttctgcggg
     4381 cgggaacatc cgggtaatca agttccatca tgtctcctgc cgcagccagg acaagcgttg
     4441 gatcagacgc tgcaagcctg catcgagggc gaagccgatc aggcccacga tcaggactac
     4501 ggccgcgagg cgatcgtatt cgagcgtgtc ccgcgcatca ttgatcgcat agccgaggcc
     4561 cgatttcacg cccagatatt ccgcgggcac gagaacgatc caggccacgc cgagcgcgag
     4621 ccgtatgccc gtcaggatat cctgcgcgat ggcgggcagg atcacggcgg tgagcagatg
     4681 aaagggattt gcgcccaaat tgcgggcgac tttgaaccat gccgggtcga tgcgccggac
     4741 gcctgcggcg gtcgaaaaaa gaacgggcca taccgctgcg acagtgatca gaaagacgat
     4801 ggctccttcc caggtcgaga aagccaggat cgcgatcggc atccacgaca gcgggctgat
     4861 cat
//
LOCUS       DATMGX010000949         3548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_345062, whole genome shotgun sequence.
ACCESSION   DATMGX010000949 DATMGX010000000
VERSION     DATMGX010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345062"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VNR65_15520"
     CDS             complement(<1..207)
                     /locus_tag="VNR65_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20128.1"
                     /translation="MQFKPLTSAQLRAARSLIRWSAEDLARETALSVATIRRAELSDG
                     ETSMTAANDLAVRRALELAGVEFID"
     gene            319..819
                     /locus_tag="VNR65_15525"
     CDS             319..819
                     /locus_tag="VNR65_15525"
                     /inference="COORDINATES: protein motif:HMM:NF017386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HWJ20129.1"
                     /translation="MDQLAFAATTYRTIRDRIRAHDPQIDEQTLADTVEGLTDLHEIV
                     QAVIRAALSDEALVLGLKCRISDMQGRLDRLQDRASKRRQIARDVMVELDLKKLNAPD
                     FTASIREGIPSLMVINEDAVPSIFWQPSEPRLKRQELAYELKQGAEIAGVALSDPEPI
                     LSVRTR"
     gene            819..1877
                     /locus_tag="VNR65_15530"
     CDS             819..1877
                     /locus_tag="VNR65_15530"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HWJ20130.1"
                     /translation="MGFSARQRLALQRNLDHRQIRTRESRGRELSYVEGWYAISEANR
                     IFGFDGWSRETIETKCVLTRENRGAFLAVYIARVRITVHAEGATVIREGHGTGEGRGT
                     SPGEVHDIALKAAETDATKRALATFGKPFGLALYGSGKTAPSPRISAPEPRVADQGQR
                     QVSLPLDNTTPIPRPSRYYGRRQDLVTRDRAQGRRQLAVQPSGDTSIVPASGELSPGQ
                     IDKSVLTIAEPKRLRDKDHLRFVASQPCLVCGRQPSDPHHLRFAQPRALGLKVSDEFT
                     VPLCRDHHQQVHQAGNELAWWHDLNINALEIAKGLWEESRMKANPTAIQSPPLQPPTD
                     PTQAAAHQDASKSPNEPN"
     gene            complement(2072..>3548)
                     /locus_tag="VNR65_15535"
     CDS             complement(2072..>3548)
                     /locus_tag="VNR65_15535"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HWJ20131.1"
                     /translation="LGGGLSLYDNADTSFTFQNPWNAVDTATRPSNNLYSLAPSNKAE
                     SISVQGGVGLPFNSRYMGTFQYTNLQVDDANLPWTNNTAYGVTPAFLTPSRNASTTLS
                     NNVLHTQITSDLKSTLKYRYYNYNTKDDSPVVVFGSVPLPWCGNPDSASSCNTTNEMR
                     YPSNFTKQNADAQLDYRPWKWLNVGASYDWERWDRDFRNVATTTENGGKVFGDAKLGG
                     FSMLRASLLYAQRRYDNYVNFDGTNIDQYRMNDLANRDRTKGMVSWAVDVTHEITITP
                     NGGFTYDDYKTDIEFFTPGEIGLKKTKSWNAGIDGTFNVAPGVALFASYNFEDGYRQV
                     YENAAIPKANVETTDLNHTIILGGKFTLIPAKLFLDANYTYTRSTSEWDLGCTPAGCQ
                     YPGMAVYPDVHNTLNRFDVQAKYMFDDAFMKSAGFAGKAYVKARVLWEKNSNDSWQSL
                     QNQYGYLVNPGNATTAYSIWMANGNPNYDVVLGQLSFGVKW"
BASE COUNT          746 a         1071 c         1011 g          720 t
ORIGIN      
        1 gtcgatgaac tcgacgccgg ccaactccaa ggcccgccga accgccaggt cgttagccgc
       61 cgtcatggac gtctcgccgt cggacagctc ggcccggcgg atggtggcaa cactcagtgc
      121 tgtctctcgg gccaggtctt cggcactcca ccggatgaga gagcgggcgg ccctgagttg
      181 agcgctggtt agcggcttaa attgcatgtt gactacttta gaagcttatg ctatgtttca
      241 tatcatatgc atcttatcag catagatgca ggtttagccg acaccatcct taatcaccca
      301 gcctagtgga gtaaaccgat ggaccagcta gcgttcgccg ccaccaccta ccgcaccatc
      361 cgcgaccgca taagggcgca cgacccccag atcgacgagc aaaccctcgc cgataccgtg
      421 gaggggctca ccgacctgca tgaaatcgtc caggccgtca tccgagcggc cctaagcgat
      481 gaggccctgg tattagggct caaatgccgg atttcggaca tgcagggccg ccttgaccgg
      541 ctgcaggacc gggcctccaa acgccggcaa attgcgcgcg atgtgatggt cgagctcgat
      601 cttaaaaagc tcaacgcgcc cgattttacc gcctcgatcc gcgagggcat cccctcgcta
      661 atggtgatca atgaagacgc ggtgccgagc atcttttggc agccgagcga gccgcggctc
      721 aagcgccagg aattagccta cgagctcaaa cagggggccg aaatcgctgg cgttgccctc
      781 tccgacccgg aaccgatcct gagcgtgagg acacggtgat gggcttctca gcaagacaaa
      841 ggctggcgct gcagcgcaat cttgatcacc ggcagatccg cacccgcgaa tcccgtggtc
      901 gcgagctctc ctatgtcgag ggctggtatg cgatctcgga ggccaatcgg atcttcggct
      961 tcgacggctg gagccgggaa accatcgaga ccaaatgcgt cctcacccgc gagaaccgag
     1021 gggccttctt agcggtctat atcgcccgag tgcgcatcac ggtgcatgcg gaaggagcaa
     1081 ccgttatccg tgaaggtcac ggcacgggtg aagggcgtgg aacatcaccc ggcgaggtgc
     1141 acgacatcgc cctcaaagct gcggaaaccg atgccaccaa acgggcgctc gccacgttcg
     1201 gcaagccgtt cggattggca ctatacggga gtggcaagac tgccccctcc ccgcgaatct
     1261 cagcgccaga gccgcgtgtc gctgatcaag gccagcgcca ggtcagtctg ccacttgata
     1321 acaccacccc gatcccgcga ccgtcccgct attatggccg ccgccaggat cttgtgaccc
     1381 gggatcgcgc tcagggaaga cgacaactgg cagtccagcc atcaggcgat acctcgatcg
     1441 tcccggcatc gggcgaacta tcaccggggc agatcgacaa gagcgttctc accattgccg
     1501 aacccaagcg gcttcgcgac aaagatcatt tgaggtttgt ggcgtcacag ccctgcctcg
     1561 tctgcggccg tcagccctcc gacccgcacc atctgcgctt tgctcaaccc cgagccttgg
     1621 gtctcaaggt cagcgatgag ttcaccgtcc cgctctgccg cgaccatcat caacaggtgc
     1681 atcaggccgg caacgaactg gcgtggtggc acgacttaaa tattaacgcg ctggagatcg
     1741 ccaaaggact ttgggaggaa agccggatga aggccaatcc gaccgcaatt caatcacctc
     1801 cgctgcaacc gcccaccgat ccaacccagg cggccgcgca tcaggacgcg agcaaatcac
     1861 caaatgaacc caattgatgc gcgaccgaat gttctcatat cagtccttca ccaacacgga
     1921 cacttaatcc ctccggatca aaatctacgc tggttcgtca ctatcaatat ccagggtatt
     1981 gcacagagca aaccggcggt ccaaacgaaa ggagcggccc gaaggccgct ccttgatagc
     2041 gattgacgtt agcggtctgg ctgcagccag ttcaccattt cacgccgaac gacagctggc
     2101 caagcaccac atcgtagttc gggttgccgt tggccatcca aattgaatac gccgtcgtcg
     2161 catttcctgg attgaccaag tagccgtact ggttttgcag cgactgccag gagtcgttcg
     2221 agttcttctc ccacagtacc cgagccttta cgtaagcttt tccggcaaag cccgcacttt
     2281 tcatgaatgc gtcgtcgaac atgtatttcg cctgcacgtc aaaacggttc aaggtgttgt
     2341 gcacgtccgg ataaaccgcc atccctgggt actggcaacc ggccggggtg cagcccaaat
     2401 cccactccga tgtagagcgg gtgtaagtgt agttcgcgtc gaggaacagc ttggcaggga
     2461 tcagcgtgaa cttgccgccc aggatgatcg tgtggttgag gtctgtcgtt tcgacgttgg
     2521 ccttcggtat ggcggcattc tcgtagactt ggcgatagcc atcttcaaaa ttgtacgacg
     2581 cgaacaaggc cacgccgggg gccacattga aagttccgtc gatgcccgcg ttccacgatt
     2641 tcgtcttctt gaggccgatt tcacccggtg taaagaattc gatatcggtc ttgtagtcgt
     2701 cgtaggtgaa gccaccgttc ggcgtgatcg taatctcgtg ggtcacgtcg accgcccagg
     2761 ataccattcc cttggtgcga tcgcggttgg cgagatcgtt catccggtac tgatcgatat
     2821 tggtcccgtc gaaattgacg tagttgtcgt agcgccgctg ggcatacagc aggctcgccc
     2881 gcagcatcga gaagccgccc aacttcgcat cgccgaagac cttgccgccg ttctcggtgg
     2941 tagtggcgac attgcggaag tcgcgatccc agcgttccca gtcgtagctc gcgccgacgt
     3001 tcagccattt ccagggacgg taatcgagct gggcgtccgc gttctgcttg gtgaagttgc
     3061 tcggatagcg catttcgttg gtggtgttgc aactcgatgc cgaatcgggg ttcccgcacc
     3121 acggcagggg aacggaacca aaaacaacga ccggcgagtc atccttggtg ttgtagttgt
     3181 agtagcggta tttcaatgtc gatttcagat ccgaggtgat ctgcgtatgc agcacgttgt
     3241 tcgacagcgt cgtgctcgcg ttacggctgg gcgtcaagaa cgcgggcgta acaccatacg
     3301 ctgtattgtt tgtccacggc aggttcgcgt cgtcaacctg caagttcgta tattggaacg
     3361 tgcccatata gcggctgttg aacggcaggc caacgccacc ctgtacactg atgctttctg
     3421 ccttattcga cggcgccagc gagtacaaat tattgctggg ccgggtcgcg gtgtcgaccg
     3481 cattccacgg gttctggaag gtaaatgaag tgtcggcgtt gtcgtagagc gacagcccgc
     3541 cgccaaga
//
LOCUS       DATMGX010000950         2417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_26260, whole genome shotgun sequence.
ACCESSION   DATMGX010000950 DATMGX010000000
VERSION     DATMGX010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26260"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1698
                     /locus_tag="VNR65_15540"
     CDS             259..1698
                     /locus_tag="VNR65_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HWJ20132.1"
                     /translation="MRRFVEGLDREQSTLFPEYLEEWVDEDNPVRAIDVFVDELELGE
                     LGFNGVDPEITGRPSYHPSVLLKLYIYGYLNRVQSSRRLEREAGRNVEVMWLTGRLAP
                     DHKTIADFRKDNGRAIREVCARFVMLCRSLGLLTGASVAIDGSKFKAVNNRDKNFTRG
                     KVERRRVQLEDSVARYLAQLDTADLQEPSEALAAKTAHLKEKLVKLEREMQRLAAMEK
                     LMLASPDQQISLTDPDSRSMATSGRGSGVVGYNVQVAVDTDHHLIVTHEVTNSGSDRA
                     QLANIASQAKEILGVDELEAVADRGYFSGEEILACDKAGIAVTLPKPMTSGIEARGRF
                     GKQDFVYLGDEDVYRCPAGEKLKYYYTNEENGQRLRRYWTNACRHCALKHRCTTGTQR
                     RITRWEHEHVLEAAQKRLDENPQAMRQRRETVEHPFGTLKMRMGATHFLMKRLPKVAT
                     EMALHVLAYNLTRVINIIGIQPLMAAMRA"
     gene            complement(1967..2155)
                     /locus_tag="VNR65_15545"
                     /pseudo
     CDS             complement(1967..2155)
                     /locus_tag="VNR65_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          511 a          675 c          736 g          495 t
ORIGIN      
        1 ttcgagcttg agaaagaaac cgtcagaggc cgcctcagca ggcggcctct ttcatttcaa
       61 acctgtcgtg cgatgtcgcc tattggcact taacggacaa accgcccgcg cccgagtttg
      121 tcgcctattg gactaacaac ggacaaagtc gattttggcc ggggatggtt tgtccgctta
      181 tgaccctggc tgtgtaaaaa cgcgtggttg gatttgattc tcgcgataag attctcgtgc
      241 gatttgcggg aggatcagat gcggcgcttc gttgaagggt tggatcgcga gcaaagcacg
      301 ctttttcccg aatatctgga agaatgggtt gatgaggata atcccgttcg agcgatcgac
      361 gttttcgtcg atgaactcga actgggcgag cttggtttca acggtgttga tccggagatc
      421 accggccggc cgtcgtatca tccgtcagta ctgctgaagc tctacatcta cggctatctc
      481 aatcgggtcc agtcgagccg ccggcttgag cgtgaagctg gccgcaatgt cgaggtcatg
      541 tggctgacgg gccgccttgc tcccgaccac aagaccattg ccgactttcg caaggacaac
      601 ggccgggcca tccgcgaggt ctgtgcccgg ttcgttatgc tttgccgcag cctaggctta
      661 ttgacgggcg ccagcgttgc tattgatggc agtaagttca aggcggtgaa caaccgcgac
      721 aagaacttca cgcgcggcaa agtggaacgg cggcgcgtgc aattggagga tagcgtggcg
      781 cggtatttgg cgcagctcga cacggccgat ctgcaggagc cgtctgaggc gttggccgcg
      841 aagacggcgc atctaaagga gaagctggtc aagctggaac gcgagatgca gcgcctggcg
      901 gcgatggaga agctgatgct ggcgtcaccc gatcagcaaa tctcgcttac cgatcccgac
      961 agccgctcga tggcaaccag tgggcgcggc tccggtgtgg ttgggtacaa tgtgcaggtc
     1021 gcagtcgata cggaccacca cctgatcgtc acgcacgagg tgacgaatag cggttcggat
     1081 cgggcgcaac tcgcaaacat tgcctcccag gcgaaggaaa tccttggcgt cgacgaactg
     1141 gaagccgttg cggatcgcgg ctacttcagc ggcgaggaaa tcctcgcgtg cgacaaggcc
     1201 ggaatcgccg tgacgctgcc aaaaccgatg acatcaggga ttgaggcaag aggccgtttc
     1261 ggcaagcagg acttcgtcta tctcggcgac gaagatgtct atcgctgtcc ggctggcgaa
     1321 aagctgaagt actactacac gaacgaggag aacgggcaga ggttgcggcg ctattggacg
     1381 aatgcgtgcc gccactgtgc gctcaaacat cgctgcacga ccggcacgca acggcgcatc
     1441 acgcgctggg agcatgagca cgtgctggag gcggcacaga agcgtcttga tgagaacccg
     1501 caggcgatgc gccaacgccg cgagacggtg gagcatccct tcggcacgct gaagatgagg
     1561 atgggggcga cgcacttcct gatgaagcgt cttccgaagg tcgccaccga gatggcgctg
     1621 cacgtgctcg cctacaatct cacgcgcgtc atcaacatta tcggcatcca gccgctcatg
     1681 gcggcgatga gggcatagtc agaccccgaa aaaggcgcca ggtcttcact gtgcgaaggc
     1741 cagaatctgc actcctccca aggcgttttt acacggccaa gacccataag cgacattgcg
     1801 gagccaaata actgcgtgca gttcatcaat gcagtcggta ggccttgtgc agcgccggtg
     1861 aaagctgaaa gccccgtctg gtttcttctc cgccattcca gagttcatcc gggaacatgc
     1921 tggcaaattg acctgacgga ttttttgtac cagcgcggtt gagagactat tgcatttgtg
     1981 cggcctgatc tagcggtgtc aggaacgggt tcatggcctg tggcgcaggt ggccggtatc
     2041 cgagcgatga gtgaggcctg atcgtgttgt attgcttccg ccattgctcg atgacaatct
     2101 gtccttcttt caggctgtag aagatctctc cattgagcag ctcatcgcgt agttctgctg
     2161 cgatatctct gcggcccggc aggcctctgg ggcggatttg ccctgcgcca tcgacacttc
     2221 aatctggcga agcagcgtca caatctgctc cgcgccgaac ctctacttcg gcatgaccaa
     2281 agctcctttc gaagacagct tctctcaaac cgcctggtac aaaaaggccc ggtcaggtca
     2341 gatcccgcgg cgcttgacca gcgcatcgct gaaatctgcg accggctgaa ggggaacgct
     2401 cccgtcacca tgcgcgt
//
LOCUS       DATMGX010000951         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_208238, whole genome shotgun sequence.
ACCESSION   DATMGX010000951 DATMGX010000000
VERSION     DATMGX010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208238"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VNR65_15550"
     CDS             complement(<1..357)
                     /locus_tag="VNR65_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20133.1"
                     /translation="MKKTVQAFSVAIIGALTLGGAVELAQAECYVQAPLRPVHRSYIR
                     RDVVEPGVYEVGRRPSVYGWTHQAVAQPGAVIWHEEPSVYRTVPVRVRQRGGSAWRER
                     CIHGQAGVCRVHVPPSY"
     gene            complement(500..655)
                     /locus_tag="VNR65_15555"
     CDS             complement(500..655)
                     /locus_tag="VNR65_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20134.1"
                     /translation="MRQLGFSDRDIWDIAAVAAFFNMSNRLASAVDMRPNAEYYSMAR
                     TKPEETV"
     gene            complement(692..1090)
                     /locus_tag="VNR65_15560"
     CDS             complement(692..1090)
                     /locus_tag="VNR65_15560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HWJ20135.1"
                     /translation="MSESTPVTALKLPEAELDLELRDYFRNCEEKLGFIPNVLKAYAF
                     DNKKLKAFIDLSDDLMLGESGLSKLEREMIAVAVSAINHCHYCLTAHGAAVRYRSKDP
                     MLGDTVEHNYRAAAITPKQKAMLDFAVKLT"
     gene            1212..>2126
                     /locus_tag="VNR65_15565"
     CDS             1212..>2126
                     /locus_tag="VNR65_15565"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20136.1"
                     /translation="MALSAETSSVSKLSAIIQSLSRQTGDGLAARFAKHLFNEVDTAE
                     FETYGTSDLVALAAGAFESFRQRQPGKPTIVLRQRFTGETELLAVDIVNDDMPFLLDS
                     VLGALRELGLVPELVAHPIFEVQRSREGILSALQPAAPSPHGIPRESFIHLQIRKLGP
                     QPPSAEIEQALADVLSDVRTVVSDFPAMTARLDLAIEQFEKNPPPATAQTNSESAAFL
                     RWLSVNNFIFLGIREYGYAGDQEHGELVPKPELGLGLLRNDEVSVLRQSADEHHLTPQ
                     GRAFFLNSPPVIVVKANSRSTVLRRVHMD"
BASE COUNT          426 a          645 c          596 g          459 t
ORIGIN      
        1 atagcttggc ggcacgtgaa cgcggcaaac gccggcttgc ccgtgaatgc accgttcgcg
       61 ccaggccgag cctccgcgtt gacggacgcg cactggaacg gtgcggtaaa cggacggctc
      121 ttcgtgccag atcactgcgc ctggctgagc cacggcttga tgggtccagc catagacgct
      181 ggggcggcgt ccgacctcat agacccccgg ctccacgaca tcgcgccgga tgtagcttcg
      241 atgcacagga cgaagcggcg cttgcacata gcattccgcc tgcgcaagct cgaccgcgcc
      301 tcccaaggtc aatgccccta tgatcgcaac gctgaacgct tgcaccgtct tcttcatatc
      361 cgctccatcg catcttcgtg cgagaaaatt agccgcatgc tgctaagaaa cgtataacct
      421 gtgttcggtt tggcggctat gagtggcaac gaaaactgag acactattgc gcgcgggcaa
      481 cagtcattgt taatttcttc tataccgttt cctcaggctt ggtcctggcc atgctgtaat
      541 actcggcgtt gggccgcata tccacggcgc ttgcgagcct gttcgacatg ttgaagaagg
      601 cggcaacagc ggcgatgtcc cagatgtcgc gatccgagaa gccgagttgg cgcaagatgt
      661 cccggtcggc atcctcgatc ttatccgaag cctacgtcaa cttgacagcg aagtcgagca
      721 tggccttctg ctttggggtg atcgcggccg cgcggtaatt gtgctcgacc gtgtctccaa
      781 gcatggggtc cttggacctg tagcgcaccg cggccccatg agcggtgaga caataatggc
      841 aatgattgat ggcgctgacc gcgaccgcaa tcatctcccg ctcgagcttc gaaagcccgc
      901 tctcgccaag catgagatcg tccgaaagat cgatgaacgc tttcaacttc ttattatcga
      961 acgcataggc tttgaggacg ttcggtatga agccgagctt ctcctcgcag ttgcgaaaat
     1021 aatcgcgaag ctccagatcc agctcagcct cgggaagctt caatgccgta acgggtgtac
     1081 tctcactcat cgttcgcgcc tctcgtcgaa gttgtgggat taagccgcag ttacccttga
     1141 tatcgtatta gagattggta ccgcttcgca attcgctttg aaggtgtgcc cgcagggatt
     1201 ggagcaacag catggcctta tcggctgaaa cctccagcgt cagtaaacta tcggcgatca
     1261 tccaatcact ttcccgccaa acgggggacg gccttgcggc gcgtttcgcc aagcaccttt
     1321 tcaacgaagt cgacaccgcc gagttcgaaa cgtacggcac aagcgatctc gtcgctctcg
     1381 ccgccggcgc ttttgaaagc ttccgtcagc gtcagccggg aaagccgacg atagtccttc
     1441 ggcaacgctt caccggcgag accgagttgc tcgcggtcga catcgtcaac gacgacatgc
     1501 ctttccttct cgactcggtg ctcggcgcat tgcgggagct tggccttgtg cctgagcttg
     1561 tcgctcatcc gatttttgag gtgcagcgca gccgcgaagg catactcagc gctcttcagc
     1621 ctgcagcacc gtcgcctcat ggcatcccgc gagaaagctt catccatctt caaatcagaa
     1681 agctcggccc gcagccgccc tccgcggaaa tcgagcaggc gctggccgat gtgctgagcg
     1741 atgtgagaac ggtcgtttca gactttcccg cgatgacggc gcggctcgac cttgcgatcg
     1801 agcagttcga aaaaaatccg ccgcccgcaa cggcgcagac caattccgag tcggcggcgt
     1861 ttcttcgctg gctgtccgtt aataacttca ttttcctcgg aatccgtgag tatggctacg
     1921 cgggcgacca ggagcatggc gagcttgttc ctaagccgga gcttgggctg gggctgcttc
     1981 ggaatgatga agtctctgtg ctccggcaaa gcgctgacga acatcacctg acgccacagg
     2041 gccgcgcatt cttcctgaac tctcctcccg tcatcgtcgt caaggccaat agcaggtcga
     2101 ccgtgctgcg ccgtgtgcat atggat
//
LOCUS       DATMGX010000952         2864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_32037, whole genome shotgun sequence.
ACCESSION   DATMGX010000952 DATMGX010000000
VERSION     DATMGX010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32037"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..792)
                     /locus_tag="VNR65_15570"
     CDS             complement(208..792)
                     /locus_tag="VNR65_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20137.1"
                     /translation="MPLQGSDIPHPQRRRAKRSAAAGRASRHGLTRRPAAPTAATRLP
                     QRPDPSIRSEAIPLFFIGQNKDGFWVARDAGGGNGGIFLLKHSALQFANTNTQPWGCA
                     TMFVEERFELDIENKGNPLVAHLGAARRLVSRLAQRLTASAGKMAVAGRMTIARFSRA
                     LAEKRMHRAAIERELFRGRYKHASKNDDDLPIVR"
     gene            complement(798..1058)
                     /locus_tag="VNR65_15575"
     CDS             complement(798..1058)
                     /locus_tag="VNR65_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20138.1"
                     /translation="MTALVLENLAVLPVQTQSLRMYLARSALDALVSARANRTVPEWQ
                     IRQVQREIDRCSRRMRGSTAQQIGTKSAAASSRHNAQSTRVR"
     gene            complement(1242..1508)
                     /locus_tag="VNR65_15580"
     CDS             complement(1242..1508)
                     /locus_tag="VNR65_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20139.1"
                     /translation="MPHAPDIPHFGSAQALTRFFLRLVILSIFAVLGSQGFGKTLESL
                     LVLAVFYCVVVAAIRREEPFGPVLTHFDEAAAYAVIARLAAWTS"
     gene            complement(1529..1999)
                     /locus_tag="VNR65_15585"
     CDS             complement(1529..1999)
                     /locus_tag="VNR65_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20140.1"
                     /translation="MLSDPKHEDHLDRLMEQLRLAPALTPDLIGAVIADACIRLPVLN
                     KAGKAARLDHLIKAGAWSDAALALIELELPTWKLRRLVYDDGEWFCSLSKQPNLPIEF
                     DDTADARHEVLPLAILSAFVEARRGTSAARETSATAVPQIRPTSGYAICCDNFA"
     gene            2455..2712
                     /locus_tag="VNR65_15590"
     CDS             2455..2712
                     /locus_tag="VNR65_15590"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF011342.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HWJ20141.1"
                     /translation="MHVNASRQSVDLVAERELGSQGSAPCSTSSCWQWVLSSSRWRLA
                     TATPAIGFKGAAMLIDYSLAGLVTAGLLIYLTYALLRPERF"
BASE COUNT          575 a          800 c          890 g          599 t
ORIGIN      
        1 acaagaaggg tggcgaagat tgcgaacaca aaaatgaccg gataagcgcg cttcgccatc
       61 gcgacagtcc atcgcaacgc actcagccac actgtgctca gatccgaggg cctggatgac
      121 gacggactca tgatctgcgt aacagtcagg gggcgagagg agattggcat gtcattgctc
      181 cagatcattg gtcgctcctg cttgcactca gcggacgatt gggaggtcgt cgtcgttctt
      241 cgatgcgtgc ttgtaacggc cgcggaataa ctccctttcg attgccgccc gatgcatgcg
      301 tttctcggca agagcacgtg agaaccgtgc aatggtcatc cgtcctgcta cggccatctt
      361 gcctgcggag gcggttagtc gctgcgcaag acgcgatacg agccgccttg cggcgccaag
      421 gtgagcgaca agcggattgc ctttgttctc gatatcgagt tcgaagcgtt cctctacaaa
      481 catcgtggcg catccccaag gctgggtatt tgtgttggcg aactgcaggg ctgagtgttt
      541 tagcaagaag atgccgccgt tacctccgcc ggcgtcgcgg gcaacccaga acccgtcttt
      601 gttctggccg atgaaaaaca gggggatcgc ctcactgcga atgctgggat cgggtcgctg
      661 cggcaatctc gttgccgcgg tgggcgccgc cggccgccgc gtgaggccgt gacgtgatgc
      721 gcggcccgcg gcggcggagc gttttgctcg ccgccgttgc gggtgcggaa tgtcggagcc
      781 ttgtagtggc atggtgatca tcgaacccgt gtcgattgcg cgttgtgacg agaggacgca
      841 gcagctgact ttgtgccgat ctgttgcgca gtgcttccgc gcattcgccg gctgcagcga
      901 tcgatttcgc gttgcacttg gcgtatctgc cattcgggga cggtgcggtt cgcgcgtgcg
      961 ctgacgaggg cgtcgagtgc cgagcgcgcc aagtacatcc gcaggctttg cgtctggacc
     1021 ggcaggacgg cgaggttttc gagcacaagg gcagtcatcg aagtctctcc ttgttgttct
     1081 tgcggaagcc ctgcctccgt gccgccaatg tgctcacgac cgcatatgaa ttcgagacgg
     1141 aacggcgctg catctcataa aggcgatata aagagaccct gcactgcacc ggatcgctgc
     1201 catgaatttg attggcgctt gccatcggtt gttttgccaa actacgaagt ccacgcagcc
     1261 aggcgggcaa ttacggcgta tgcggctgcc tcatcaaaat gagtcagtac cgggccgaag
     1321 ggctcctcgc gtcggatcgc agcaacgacc acgcagtaga acaccgccaa caccagtagg
     1381 ctctcaaggg tcttcccgaa tccttggctg ccgagcaccg cgaagatact gaggatgacc
     1441 aagcgcagaa aaaagcgggt cagcgcttgg gctgacccga agtgggggat gtccggagca
     1501 tgcggcatgc tgactcggcg gaggccgctc aggcgaagtt gtcacaacaa attgcatagc
     1561 ctgatgttgg ccggatttgc ggcactgccg tggcgctggt ttcgcgcgca gcgctagtcc
     1621 cgcggcgcgc ttcaacgaac gcacttagga ttgccagagg caatacttca tgcctcgcgt
     1681 ccgctgtgtc atcgaattca atcggcagat tcggttgctt ggagagggag cagaaccatt
     1741 caccgtcgtc gtagacaagg cgacgaagct tccatgtcgg cagttcgagc tcgattagag
     1801 caagcgccgc gtcgctccaa gcgccagcct taatcagatg gtcgagccga gcagcctttc
     1861 cggccttgtt cagcaccgga aggcgtatgc aggcgtccgc gatgacagcg ccgatgagat
     1921 cgggggtgag tgccggcgcg agccgaagct gctccatcag ccggtcgaga tggtcctcgt
     1981 gtttcggatc ggacaacatg acatcatctc ataggaacaa cggtcttagc gaccgcagcc
     2041 gtaaaatggg gtcaaacgtc ataaagaagc gagagggacg gggacgccct gatatacgga
     2101 tgtcataaga atcggaatgg atctttataa agatccacta ggcgcgcgag ctcggcaggt
     2161 ttgcgcttct gcaagcacgg ccacacgaag ctccgatgca ctgggcgtgg tgcccgcgtc
     2221 tgcatccgag cggcgtcatc gctgccacag gcgttgctgg ccgcaaggcc atagcaaact
     2281 atcgtgtcat tccaagctgt tattgccgaa aatgaagcgt ggcatggggc gatcatgacg
     2341 ccgcatcttt atgagattcc tataacgcgg gcttcgcttc catctggaaa ttttatggcg
     2401 atgagtgcca cgtaagcgcc cgcaggagac ggcaaaaata tttagcaaag tttgatgcac
     2461 gtcaatgcgt cccgccagtc ggtcgatctc gtggccgagc gggaattggg ttcgcaaggg
     2521 agcgctccat gctcgacttc gtcatgctgg caatgggtct tgtcttcttc gcgctggcga
     2581 ttggctacag ctacgcctgc gatcggcttt aagggggctg ctatgctcat cgactattca
     2641 ctcgccggcc tcgtgaccgc ggggctgctg atctacctca cctacgctct gctgcggccc
     2701 gagcggtttt agccgcgtga ccccgaaaag cgggggccgg ttttcgggaa cgaaaagaac
     2761 atgcgccaaa tgagagatgt gccatgactg tgattggctg gattcaaatt ctgctttact
     2821 gcgcgatcat cgtcgcgatc accccgttgc tcggcggcta tatg
//
LOCUS       DATMGX010000953         2005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_456033, whole genome shotgun sequence.
ACCESSION   DATMGX010000953 DATMGX010000000
VERSION     DATMGX010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456033"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1562
                     /locus_tag="VNR65_15595"
     CDS             183..1562
                     /locus_tag="VNR65_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HWJ20142.1"
                     /translation="MQTECSQDSLDFASLGSRKVTAAFDGGAITSNAGALLLREADRV
                     LGLSQQVAACFTDSRRQDRVEHAVETLLAQRIHGIALGYEDLNDHDELRHDPVLGLVS
                     GKAEARRSDCAVLAGKSTLNRLEHAPKTDDDRYRKLSVDEGAMKRLFVSVFLKSHATP
                     PERIILDLDATDDPIHGDQEGRFFHGYYKCYCYLPLYIFCGRELLLAKLRSANMDASA
                     GAKEEVALIVAQIRERWPNVEIWLRADSGFCREEVMAWCENNGVHYVFGLARNARLEA
                     SIADELAEAEAKAKESGKPERIFKELRYQTHKSWSRERRVVAKAEHLLKGPNPRFIVT
                     SLGCEAFEAQELYEKIYCARGEMENRIKECQLDLFADRTSTASLRANQLRLWFASLAY
                     VLVTALRRMALQGTELAQATAGTIRLKLLKLGAQVTVSVRRVKIAITSACPLKSVFAN
                     AHRRLCLTA"
     gene            complement(1821..2005)
                     /locus_tag="VNR65_15600"
                     /pseudo
     CDS             complement(1821..2005)
                     /locus_tag="VNR65_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508717.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
BASE COUNT          434 a          611 c          575 g          385 t
ORIGIN      
        1 gaacctggtg cagaaattca ggacgtcatc acacattcaa actaagtaac ttttcaggtt
       61 cctttgactg gtaccgcatc tcgctagccc gcatatctca cgagagttag gtgcatcggg
      121 gctctgaatc agcgaaagtt cttctgcaac aaatgcttcg gctagagcag aggagatccc
      181 cgatgcagac agagtgtagc caggattccc tcgacttcgc cagcctcgga agccggaaag
      241 tcacagcggc ttttgacggc ggggccatca cctcgaacgc aggtgcgctt ttgttgcgcg
      301 aggccgaccg cgtcctcggc ctgtcccagc aggtcgcggc ctgcttcaca gatagccgca
      361 ggcaggaccg ggtcgagcac gcggtcgaaa cgctgctcgc ccagcgcatt catggcatcg
      421 cgcttggcta tgaagacctc aacgatcacg atgaactgcg ccatgatccc gtgctgggcc
      481 ttgtctcggg caaggctgaa gcgcgccgct ccgattgcgc tgtgctggcg ggaaagtcca
      541 cgttgaaccg gcttgaacat gcgccaaaga cagacgatga ccgctatcgc aagctcagcg
      601 tggacgaggg cgcgatgaaa aggctgttcg tcagcgtctt tctcaagagc cacgcaacgc
      661 cgcccgagcg cattatcctc gatctcgacg cgaccgacga tcccatccat ggcgaccagg
      721 aaggccggtt cttccacggc tattacaaat gctactgcta tctgccgctg tacattttct
      781 gcggccggga gctgcttttg gccaagttgc ggtcggcgaa tatggacgcc tcggctggcg
      841 ccaaggagga ggtcgccttg atcgtcgcgc aaatccggga gcgctggccc aacgttgaaa
      901 tctggcttcg ggcggattcc ggcttctgcc gggaagaggt gatggcgtgg tgcgagaata
      961 acggggtcca ctatgtcttc ggccttgccc gaaatgcgcg tctcgaagct tcgatcgcag
     1021 acgaactggc tgaggctgag gccaaagcca aggaaagcgg caaacccgaa cgcatcttca
     1081 aggaactgcg ctaccaaacc cacaagagct ggtcccgaga gcgccgggtc gtggcgaagg
     1141 cggagcacct cttgaaaggt cccaatccgc gctttatcgt cacctcgctg gggtgcgaag
     1201 cctttgaagc acaggagctt tacgagaaaa tctattgcgc tcgcggcgag atggagaacc
     1261 gcatcaagga atgtcagctc gatctcttcg cagaccgaac ctcgacggca agcttgcgtg
     1321 ccaatcaact gaggctgtgg ttcgcctcgc tcgcttacgt gctggtcacg gccttgcggc
     1381 gcatggcctt gcaaggaacc gaactcgcgc aagccacagc cggaaccatc cggcttaagc
     1441 tcttgaaact tggcgcgcag gtcacagtca gcgtccgtcg cgtcaaaatc gccatcacct
     1501 cggcttgtcc gctgaaaagc gtgttcgcca acgcgcaccg gcgattatgc ctgaccgcat
     1561 gaagcagcgg agccaagccc gccccacaaa atacattgcg aaacttcagg cacccggttc
     1621 tccggcgcaa aagacgtcac tatgaaagag cctgcctgct tgcaatggcc tcgaatggaa
     1681 cgccactcgc caggcgtgcc cctggtgaga aatgcgggta agcgcctatc cgcttttgat
     1741 ggaatcaaaa gcgggctcta agttattgtt ttgtcgtgcg tcttatcgga aaaccgcttc
     1801 gcacttttcc ggacgcactc taaggttgac ggatcgccgg tgttaccctc gaacacctcg
     1861 acggcaaccg gacagccatc ggcggcgcag agcaggccga agacgatttg cagcttgtcc
     1921 cgcttgccat cccaaaccgc cccagctcac agtggcggcc ctcaagatag ctcgacgtga
     1981 gatcgtaccc ccggacgcac cccat
//
LOCUS       DATMGX010000954         2919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_53185, whole genome shotgun sequence.
ACCESSION   DATMGX010000954 DATMGX010000000
VERSION     DATMGX010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53185"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..393)
                     /locus_tag="VNR65_15605"
     CDS             complement(151..393)
                     /locus_tag="VNR65_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20143.1"
                     /translation="MPTVRDDTVFTHGHFEKASYNYRAEEAAKAKAFINFLMQDRLFL
                     ALYRSNDPKVRAAALEKLNEAHHHAYGDAQLHQRGP"
     gene            complement(445..957)
                     /locus_tag="VNR65_15610"
     CDS             complement(445..957)
                     /locus_tag="VNR65_15610"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ20144.1"
                     /translation="MIVKFRPMQAEDKELFKSFMRSLPRKDNYYLFIDVQNDHAIDRW
                     MEKIHMGQTIGVVATNGSDMIGYCNLKTNGLAWTSHVGEICISVSTAYRGRGIGKALA
                     EQIFLKARVRGLQKLWTRMAISQDAAQHMFMKLGFRTEAVLSDFVKKENGLTEDLVLM
                     SHDSGEHWSF"
     gene            complement(1058..1453)
                     /locus_tag="VNR65_15615"
     CDS             complement(1058..1453)
                     /locus_tag="VNR65_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20145.1"
                     /translation="MNLNQNNASVAVAEARRPFQVGLLFEFDEIAQTYLKAAQGSGHT
                     YLQNYLDGVVAIGDAYFTRRAILLAIHDMKEDLAAPFALQERALAELLSLKVRPAPEL
                     RLYRDIATALLRMEVVIRFQAMRDGETRH"
     gene            complement(2029..2688)
                     /locus_tag="VNR65_15620"
     CDS             complement(2029..2688)
                     /locus_tag="VNR65_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ20146.1"
                     /translation="MSAPAALRVLVADDHPVVLAGIRALLDGNPDIEIVGEAHDGPMA
                     LRMALELQPSVVVLDLSMPGLNGMEVTKLLLAERPQCKVVILTVHEDRSYLRKLIEVG
                     ASGYVLKRSVAEDLLRAIQAVAAGGVYLDPSIAVHALDRTPNKPKETADGHAELSERE
                     VEVLRLTAVGHSNKAIAHILNISVKSIETYKARAMDKLGFRSRVELVGYAVEKGWLTG
                     V"
     gene            complement(2685..>2919)
                     /locus_tag="VNR65_15625"
     CDS             complement(2685..>2919)
                     /locus_tag="VNR65_15625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ20147.1"
                     /translation="ARHSEATRAAVLLEANEKEVSMIVEDDGRGFSANEATPGDKRLG
                     LLGIRERLSLVSGTLEVESAPGHGCTLYIRVPL"
BASE COUNT          648 a          829 c          763 g          679 t
ORIGIN      
        1 gcgcttgcag gcggtcgccg aggtaatccc aaaagcggac ggtctgtttg gcgcaggttt
       61 aaaagtatcg agcttatgga tcagccgttc cgcatgaacc cagcacagcg catggtctgc
      121 gacccggaac tcggccattt agctcatccg ctagggacca cgttgatgaa gttgggcgtc
      181 accgtaagcg tgatggtgag cttcgttgag cttttctagg gcagccgcac gcactttcgg
      241 atcgttactg cgatataggg caagaaaaag ccgatcctgc atcaggaaat tgataaaggc
      301 tttggccttc gcagcttcct ctgccctgta attataggaa gccttctcga agtggccatg
      361 agtaaaaacg gtatcgtctc ttactgtggg catgatgtac tcctcagtta ctctcgttcc
      421 accgtcggct cgaagcactc gcgttcagaa agaccaatgc tctccggaat cgtgcgacat
      481 aagcacgagg tcctcggtta agccattctc cttcttcaca aaatcggaga ggacagcctc
      541 ggtgcggaag ccgagcttca taaacatgtg ttgggctgca tcttggctga ttgccatccg
      601 cgtccagagt ttttgcagcc ccctcacccg cgcctttaag aaaatttgct ccgccagagc
      661 tttgccgatt ccgcgtcccc ggtatgcggt tgaaacgcta atgcaaattt caccgacgtg
      721 gcttgtccag gccaggccgt ttgttttgag attgcaatag ccgatcatgt cgctcccatt
      781 ggtcgcgacc acgccaatgg tttgtcccat atgaattttc tccatccagc gatcgatcgc
      841 atgatcgttt tggacgtcga tgaacaagta gtaattgtcc tttcgaggaa gcgatcgcat
      901 gaagctctta aacagctctt tgtcctcggc ttgcatcggt cgaaatttca caatcattgc
      961 agtctcctcc gtcctaagag gatttcactt tgcgcggcta ccagcgtatg ctccgaacct
     1021 tgccttagac cgcgcgatca ttgacccagc attattccta atgcctggtt tcgccatctc
     1081 tcatggcttg aaatcttatg acgacttcca ttctgagaag cgcggtggcg atgtctcgat
     1141 aaagtctcaa ctcaggcgcc ggccgcacct tcagcgacag caattccgca agggctcgtt
     1201 cctgaagcgc aaacggagca gccagatcct ccttcatgtc atgaatcgca agaaggatcg
     1261 cgcggcgagt gaaataagcg tcgccaatag cgaccacacc gtccaagtaa ttctgaagat
     1321 aggtatgacc gcttccctgc gcggctttga gatatgtctg ggcgatttca tcgaactcga
     1381 aaagcaagcc cacttggaaa ggacgccgcg cttcagctac ggccacagat gcgttgttct
     1441 gattcagatt cattaagggg gctccgtttc cagctgacat tgaaggcgca ggcaaaggct
     1501 ctcagcctgc cggttgagtt tgcgggtagc cgcattcgtt cgtgactgat cctattggca
     1561 cgcttaagaa aacgcacaca aacccaagtc gatcgggact ggccaagttc atccgatcta
     1621 gcatcaagtg caaattcggt cagataagct gcctggagca ctttgatcga aatctaatct
     1681 cagttgaggc caagagtaga cgaagctcac ctacccagca acaacgaaaa tatacgcgga
     1741 gtgtcagaat aagacgaacg tgttgggtgg gtttttcctg acatgatact tggtggttat
     1801 cgtggtatgt ggtcagccgg cttgaagcta gaagcgcttc ccacattctc gctaagggcc
     1861 atccaggcat aaacttaggg ggctccacgg cccccctttt ttgttgatac ccattggagt
     1921 cttgcgattt tggacaatga gccggctcgg aacgcggacg cgaagtttgg gcctgcctcg
     1981 gagctgacag cccgccggct taagggcaag gccatgtaca gaccctcgtc agacgcccgt
     2041 cagccaaccc ttttcgaccg cataccctac aagttcaacc ctgcttcgga agccaagctt
     2101 gtccatggca cgcgccttgt aggtttctat actctttacg ctgatgttca agatatgcgc
     2161 gatcgcctta ttgctatggc ccactgcggt cagccgcagc acttccactt cgcgttcgct
     2221 cagttcagca tgtccatcgg cggtttcctt tggcttgtta ggggttctgt ccagggcgtg
     2281 aacagcaatt gaagggtcaa gataaacccc gccggccgca actgcctgga tggcgcgaag
     2341 aaggtcctcc gccactgacc tcttgagcac atagccggac gccccaacct cgatcagctt
     2401 tcgcaaatag gatcggtctt catgaaccgt cagtataacc actttgcact gaggacgctc
     2461 tgcaagcagc agctttgtga cttccattcc gttcaggccg ggcatggata aatccagcac
     2521 cacgactgaa ggctgaagct ctagggccat ccgaagcgcc atcgggccgt catgggcttc
     2581 gccaacgatt tcgatgtctg gattgccgtc cagcaaagcc ctgatgccgg caagcacaac
     2641 aggatgatcg tcggcgacga gaacgcgaag ggctgccggc gcgctcatag gggcacgcga
     2701 atgtaaaggg tgcagccgtg accgggcgcg gattcgacct ccagcgtgcc agagaccagt
     2761 gacaagcgct cccggatgcc gagcaaacca aggcgcttat cccccggcgt cgcttcgttc
     2821 gcggaaaaac cgcgcccgtc gtcttcaaca atcatgctga cttctttctc attggcctca
     2881 aggagcaccg cggcccgcgt cgcctccgag tgtcttgcg
//
LOCUS       DATMGX010000955         2896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_308392, whole genome shotgun sequence.
ACCESSION   DATMGX010000955 DATMGX010000000
VERSION     DATMGX010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308392"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1645)
                     /gene="guaA"
                     /locus_tag="VNR65_15630"
     CDS             complement(83..1645)
                     /gene="guaA"
                     /locus_tag="VNR65_15630"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWJ20148.1"
                     /translation="MHGLPITDVVLIVDFGGQFTQLIARRVREASVYSEIVPFNAAEK
                     AIAELKPKAIILSGGPASVFDPGAPQVPDAIFEAGVPVLGICYGQQGMVARLGGKVEP
                     STEREYGSALIDIKAASPLFEGVAGVGEKAPVWMSHGDRVASLPEGFASIATTRNAPY
                     AAIADERRRYYGVQFHPEVVHTPSGAIMLRNFVHKIAGCDTSWTMAAFRDAEIARVRA
                     QVGGAKVICGLSGGVDSAVTAVLLHEALGDALTCIFVDHGLLREGEAEEVEGLFRGAY
                     NIPLIHVKAAPRFLSELSGVTDPEQKRKTIGRLFIEIFDEAAHQVGGAEFLAQGTLYP
                     DVIESVSFHGGPSVTIKSHHNVGGLPERMKLKLVEPLRELFKDEVRALGRELGLPDAF
                     LKRHPFPGPGLAIRIPGAISEEKLRVLRKADAIYLEELRAAGIYDDIWQAFAVLLPVQ
                     TVGVMGDARTYDHVLALRAVTSVDGMTADFFRFPLDVLGHIATRIVNEVKGVNRVVYD
                     VTSKPPGTIEWE"
     gene            complement(1856..2053)
                     /locus_tag="VNR65_15635"
     CDS             complement(1856..2053)
                     /locus_tag="VNR65_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20149.1"
                     /translation="MGFIKAIFYLIIIGFLAIGGYWLYGAFSTAGDAPYWTEINKRLP
                     VQAKNFACEQIKKNHPNASCD"
     gene            complement(2228..>2896)
                     /locus_tag="VNR65_15640"
     CDS             complement(2228..>2896)
                     /locus_tag="VNR65_15640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HWJ20150.1"
                     /translation="GWFEVQDEGSQIAALLAGAGARMQIADLCAGAGGKTLALAAQMQ
                     NTGQIYAYDSDAHRFRPIFDRLKRAGARNVQTLPPGEDAALDALDGRMDLVLIDAPCS
                     GTGVWRRRPDAKWRLTSTQLGERVATQAALLMRADRLLKPGGTLAYITCSILPDENEK
                     QIKAFLEHNPSFSAIDVRQHATKVLSRSVTCSANHNKNGLLLTPARHGTDGFYMALLL
                     KSAT"
BASE COUNT          587 a          874 c          849 g          586 t
ORIGIN      
        1 tagccgattg ctttctctgc tatcggcagg cgcatcttgc gctaagcact tgaagctatt
       61 ctgcagcggc cctacgcaac cgctattccc attcgatcgt gcccggcggt ttcgatgtca
      121 cgtcgtaaac aacgcggttg acgcccttga cctcgttcac gatgcgcgtc gcgatatggc
      181 cgaggacatc caggggaaag cggaaaaagt ccgccgtcat gccatcgacg gaagtgacag
      241 cgcgcaacgc cagcacatgg tcataagtcc gcgcgtcccc cattacgccg acggtttgca
      301 cgggaagcag caccgcgaaa gcctgccaga tatcgtcgta gatcccggcg gctctgagtt
      361 cttccagata aatcgcgtcg gccttgcgca aaacgcgcag cttctcctcg gagatggcgc
      421 cgggaatgcg aatggcgagc cccggccccg ggaacgggtg ccgcttcagg aacgcgtccg
      481 gcaacccaag ctcgcggcca agcgcgcgaa cctcatcctt gaacagctcg cgcaaaggct
      541 cgaccagctt cagcttcatg cgctccggca aaccgccgac attgtgatgc gacttgatag
      601 tgacggatgg cccgccgtga aacgagacgc tctcgatgac gtccggataa agcgtgcctt
      661 gggccaagaa ttctgcgccg ccgacttgat gcgcggcttc gtcgaagatc tcaatgaaca
      721 agcggcctat cgtcttgcgc ttctgctcag gatcggtgac gccgcttagc tcggaaagga
      781 agcgcggcgc ggccttcaca tggatcagcg gaatgttgta ggcgccgcga aagaggcctt
      841 cgacttcctc cgcttcgcct tcgcgcaaga ggccgtgatc gacgaaaatg caggtgagcg
      901 cgtcgccgag tgcctcgtgg agcaaaacgg cggttacggc ggaatccacc ccgcccgaaa
      961 ggccgcaaat caccttcgcg ccgcccacct gcgcgcggac gcgggcgatt tcggcatcgc
     1021 ggaacgcggc catcgtccag gaagtgtcgc agcctgcgat cttgtgcacg aagtttcgca
     1081 gcatgatcgc gccgcttggc gtgtgaacga cctcgggatg aaattgcacg ccataatacc
     1141 ggcgccgctc atcggcgatg gcggcatagg gtgcattgcg ggtcgtcgcg atcgaagcga
     1201 agccttcggg cagcgaagca acccggtcgc catggctcat ccagacaggc gccttttcgc
     1261 caacgccagc cacgccctcg aataatggcg atgcggcttt gatgtcgatc agcgccgagc
     1321 catattcccg ctcggtcgac ggctcgacct tgccgccaag ccgcgccacc ataccctgct
     1381 gcccatagca gatgccgagc accggcacgc ccgcctcgaa aatcgcatcc ggcacttgcg
     1441 gcgcgcccgg atcgaaaacg cttgccgggc cgccggaaag aatgatcgcc ttcggcttca
     1501 gctccgcgat ggccttttct gctgcgttga agggcacgat ttccgagtaa acgctggctt
     1561 cgcgcacgcg ccgcgcgatg agctgcgtga actgcccgcc aaaatcaaca atgagaacga
     1621 catcggttat ggggagaccg tgcaaggggc gctcctctct cgctagatca acccaccttt
     1681 gagcgactac aggcggaaaa gttgatcgat tctgaagtgc tagagagcag cttatgcatc
     1741 caacggacgt ggctgccctg gtcataaaag aatgggtccg cggattgcgg acccatgcct
     1801 aagatagacg gattttttta agcgtccagc gcggagccct ttccgcgctc aactcttagt
     1861 cgcagctggc attcgggtgg ttcttcttga tctgctcgca ggcgaaattc ttggcctgga
     1921 ccggcagtct cttgttgatt tcagtccaat acggagcgtc gccagcggtg ctgaaggcgc
     1981 cgtagagcca ataaccgccg atagccagga agccgataat gataaggtag aagatggcct
     2041 tgatgaaacc catagcggaa ctcgcttttc ttgatggttg gtctttgacg ggtgggttgc
     2101 cgaatgagca tccccaggca cctttagatc caagttgtaa caggttcttg accggatctg
     2161 taatgcagca atgcaggaat tttgtccatt ttgcggcagg gcgatcttgg cccactatgt
     2221 tgagacatta tgtggcgctt tttaataaaa gtgccatgta aaaaccgtct gtaccatgac
     2281 gagcgggcgt taaaagaagt ccgttcttat tgtgattcgc gctgcaggtt acagaacggg
     2341 agaggacttt tgtagcatgt tgcctcacgt caattgcaga gaaactggga ttgtgctcga
     2401 gaaacgcttt gatttgcttt tcgttttcgt cgggcaggat cgagcaggtg atgtaagcaa
     2461 gcgttccacc cggctttagc aaccggtccg ccctcatcaa aagcgcagct tgcgttgcca
     2521 cgcgctcgcc aagttgggtg ctcgtcagcc gccatttcgc atcgggccgc cgccgccaga
     2581 ctcccgttcc gctgcagggc gcgtcgatca ggacaaggtc catgcggccg tccagagcat
     2641 cgagagcggc gtcctcgccg ggcggcaggg tttgcacgtt tcgcgcgccc gctcgcttca
     2701 atcgatcgaa gatcgggcgg aaccggtgcg catcggagtc gtaggcgtaa atctgccccg
     2761 tgttctgcat ctgggccgcc agggcgagcg tctttccgcc agcgcctgca catagatcgg
     2821 caatttgcat gcgcgccccg gcgccagcca aaagcgctgc aatctggctt ccttcgtcct
     2881 gcacctcgaa ccatcc
//
LOCUS       DATMGX010000956         2461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_358994, whole genome shotgun sequence.
ACCESSION   DATMGX010000956 DATMGX010000000
VERSION     DATMGX010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358994"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VNR65_15645"
     CDS             complement(<1..1219)
                     /locus_tag="VNR65_15645"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HWJ20151.1"
                     /translation="MNIAFAASECVPFSKTGGLADVIGALPPALASLGHQVTVFLPRY
                     KQTKLDNPRIVIPSITVPFDDRYRFCSVLDGGVRSGVQFYFIDYPPFFDRDSLYGTPL
                     GDYHDNAERFALFCRAVLESAKVLGTPDVFHCHDWQTALIPILLKTLYEEDPALANTP
                     CVFTIHNIGYQGLFPPEILPLLMLPWDLFTMSKLEFYGKVNFLKGAITMADYITTVSR
                     KYSQEIQTAEYGFGLEGVLRARSGTVSGILNAVDYGEWNPEGDRFIAAHYSAGDLSGK
                     AKCKADLLKEVGLAEDTQLPVVGIVSRFAAQKGFDLILQVANRLALQELIMVVLGSGG
                     REYEELFRKLNKQYPQKFAVKIAYDSALAHKIEAGSDIFLMPSRYEPCGLNQIYSMRY
                     GTVPVVRATGGLDD"
     gene            1313..2206
                     /gene="rsmA"
                     /locus_tag="VNR65_15650"
     CDS             1313..2206
                     /gene="rsmA"
                     /locus_tag="VNR65_15650"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWJ20152.1"
                     /translation="MSLIGEWKEGNVARTTSNKSVTGVKHKPKLGQNFLTDLGAAQRV
                     VEALGDVSNRTVIEIGPGRGVLTDVLVARAKHVIGIELDRVMAAQLRMRYATKPNVEI
                     LESDFVAAQFTSMVGRRPGPLHDLRPTQPETVDVIGNLPYYITSDIVLRILELHQNIE
                     RAVIMVQREVADRISAAPGSRDYGLLSATVQLFARVDKLFTLPPSAFSPPPQVHSSAL
                     RLTMTPRLEELQVEEDPFIAFLKLAFGHKRKTLLNNLRGQYEDVAIRAALKSAGLRAD
                     VRAEAMSLEKTAAVFRALPQA"
BASE COUNT          525 a          733 c          733 g          470 t
ORIGIN      
        1 tgtcgtcgag cccgccagtg gcgcgcacca cgggtacagt cccataacgc atactgtaga
       61 tctggttcag cccgcaaggc tcatagcgcg agggcatgag gaagatgtcg ctgccggctt
      121 caatcttgtg cgccaaggcg ctgtcgtaag cgatcttcac ggcgaatttc tgcggatact
      181 gtttgttcaa cttgcggaac aactcttcat actcgcggcc gccgctcccc agcaccacca
      241 tgatcaactc ttgcaaggcc aagcggttgg ccacctggag gatcagatcg aaacccttct
      301 gcgcggcaaa gcgcgacacg atgccgacta ccggcaactg cgtgtcttcc gccagtccaa
      361 cttccttcaa caggtccgcc ttgcattttg ctttgccgct gaggtcaccg gctgagtaat
      421 gcgccgcaat gaagcgatcg ccctcgggat tccattcgcc ataatccacg gcattgagaa
      481 tgccgctaac cgtgccggag cgcgctcgca acacaccctc cagaccgaag ccgtactccg
      541 cggtctggat ttcctggcta tacttccggc tgacggttgt gatgtagtcg gccatggtga
      601 tcgcgccctt gaggaagttc accttgccat agaactccag cttcgacatg gtgaacaaat
      661 cccagggcaa catgagcagg ggcaggatct cgggcgggaa aagtccctgg tatccaatat
      721 tgtgaatggt gaagacgcac ggagtattcg caagggccgg gtcttcctcg tacagcgtct
      781 tcagcagtat gggaatcagg gccgtctgcc agtcgtggca gtgaaacacg tcgggcgtgc
      841 cgagcacttt ggccgactcc agcacggcgc ggcaaaacaa cgcgaacctc tcagcgttgt
      901 cgtggtagtc gcccaacggt gtaccgtaga gcgaatcgcg gtcgaagaac ggagggtagt
      961 cgatgaaata gaattgcact ccggagcgca caccgccgtc gaggacggaa cagaagcggt
     1021 agcggtcatc gaagggaacg gtgatgctgg gaatgacgat gcgcggattg tcgagtttgg
     1081 tctgcttata acgcggcaga aagactgtaa cctgatgccc caaactggcg agcgcaggcg
     1141 gcaaggcgcc tatgacatcc gccagcccgc cggtcttgga aaagggtacg cactcggatg
     1201 ccgcaaaggc gatattcatg aaacctcccg gcgcgaacgg agaggtgctt ccgcgggcac
     1261 gccgaactgc gtaactggac tgcgcactat gatgtaagaa atgccgaaag acttgagtct
     1321 aattggcgaa tggaaagagg gcaatgtggc gaggaccacg tcgaacaaat ccgtcaccgg
     1381 cgtcaaacac aaaccgaagc tgggacagaa ttttctcacc gatttgggcg cggcgcagcg
     1441 cgtcgtggag gccctcggag atgtgtccaa ccgcaccgtc atcgagatcg gcccgggccg
     1501 cggcgtgctc accgatgtgt tagttgcgcg ggccaaacac gtgatcggaa tcgagctcga
     1561 tcgcgtgatg gcggcccagt tgcgcatgcg gtatgcgacc aaaccgaatg tcgaaatcct
     1621 cgaatcggat ttcgttgccg cgcaattcac ctccatggta ggccgcaggc cggggccgct
     1681 gcacgatctc cgtcccaccc agccggagac ggtggacgta atcggcaacc tgccgtatta
     1741 catcacctcc gatatcgttc tgcgaattct ggaactacac cagaacattg agcgcgcggt
     1801 catcatggtg cagcgggaag tcgccgatcg tatctcggcc gcgccgggca gccgggatta
     1861 tggactgctc tccgccaccg ttcagctctt cgcccgggtt gacaagctgt tcacactgcc
     1921 accgagcgct ttctcgcctc ccccgcaggt ccattccagc gcgctacggc ttaccatgac
     1981 tccccggctg gaagagctac aggttgaaga agatcccttc atcgcgtttc taaaacttgc
     2041 cttcggccat aagcgcaaga cgttgttgaa caatcttcgc ggtcagtacg aggatgttgc
     2101 cattcgagcg gcgctgaaat cggctggact gcgcgccgat gttcgcgccg aagccatgtc
     2161 gctggaaaaa acggcggcgg tgtttcgcgc cttgccacag gcgtaaactt gcctcagaga
     2221 tcgccgtcgg caggcgcgga acgtgcgcgc caagcggcgg cggtgcggtg cagcaaagga
     2281 ctgagcacca tggttaccgc agttcgtcag ccagccgttg caggacgctt ctatcccgcc
     2341 aacgcacagc acctccgggc ggaagttgag acttacacca cggcgcgcgc cgatgcttcg
     2401 gcagagtccg aatcgaggat tcgcgcctgg ggctgcattg ttccccacgc cggatacgtg
     2461 t
//
LOCUS       DATMGX010000957         3434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_435569, whole genome shotgun sequence.
ACCESSION   DATMGX010000957 DATMGX010000000
VERSION     DATMGX010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435569"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VNR65_15655"
     CDS             complement(<1..318)
                     /locus_tag="VNR65_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20153.1"
                     /translation="MPFGIAIRHRLRRHRHIRKTEKAKADEPLTSDPPAAKSERPGQP
                     PMPAPKDSEPEQPNEASKPPEPMGPPPPPLTWTASEVEAGRKDCDRRLSGVHIIFERL
                     DPIR"
     gene            complement(655..2232)
                     /locus_tag="VNR65_15660"
     CDS             complement(655..2232)
                     /locus_tag="VNR65_15660"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWJ20154.1"
                     /translation="MSIRRILLAWLAVIGLVAASPAIAADEKKPNIVIIWGDDIGQSN
                     ISAYTNGLMGYRTPNIDSIAQQGVIFTDYYGEQSCTAGRSAFITGQNVFRSGLSKVGL
                     PGADLGLRKEDPTIAELLKAQGYATGQFGKNHLGDKDEMLPTAHGFDEFYGNLYHLNA
                     EEEPELPDYPKANDPEFPNFRKNFGPRGVIHSFANSDGTQRIEDTGPLTKKRMETIDD
                     DIAARSAEFLDKQVKANKPVFLWVNFTHMHFRTHTKPESLGQAGRWVGPYADTMIDHD
                     RNVGTVLAKLKELGIEDNTIVMYSTDNGPHMNSWPDAAMTPFRNEKNSNWEGAYRVPA
                     MVRWPGKIKAGTVSNEIVSHLDWMPTLLAAAGAPDIKEKLLTGYKAGSMTYKVHLDGY
                     NLVPYLTGTENKTPRKEFFYFSDDGDLTALRYDNWKFVFAEQRAPGTLRVWAEPFTRL
                     RIPLIYNLRLDPYERANITSNTYYDWLLDHAFMLVPAQAYVGKFLGSFKDYPPRQKAA
                     SFSVDQALEMMKKSSGD"
     gene            complement(2559..3143)
                     /locus_tag="VNR65_15665"
     CDS             complement(2559..3143)
                     /locus_tag="VNR65_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20155.1"
                     /translation="MLWLAPSRAPRHVSSGLALAPDTAEEKAKNAQCVKDVCSIVLTK
                     EANGPDVNCDLAHRWDAKEIRALAESKDLSWSLGPAPCSLKISAKRADIHAAVTSPEH
                     TLKLTGKSVACVVGEEEYDVTADVSADFTLKDGVVTAISLSGSDYDGPFFIAKTLFVA
                     WGMEKRFGSFQGDMLREANRFIKNECPKALTAPK"
BASE COUNT          681 a          957 c         1003 g          793 t
ORIGIN      
        1 cctgatggga tcgagacgct caaagatgat gtgcacgccc gaaagccgcc ggtcgcaatc
       61 ctttcttcca gcttccacct ccgaggctgt ccacgtcaga ggaggcggag gcggccccat
      121 cggctcgggc ggcttggacg cctcgttcgg ctgttcaggc tcggaatcct ttggagcggg
      181 catcggcggc tgtcctggcc tctccgattt cgcagcgggc ggatcgctcg tcagcggctc
      241 gtccgccttc gctttctcag tttttcgaat gtggcggtgt cggcgaagac gatgtcggat
      301 cgcaatgcca aagggcaagg cgcttttcgc aaatcccgaa attggtgggg ctatgccgtg
      361 agcgtttgac ggaagacagg aaaaagatac agcaagtgcc gtgatgcacg ctaatgctat
      421 ggctgtggcg ctgggcgaag ttgtcatacc gcgagcggct atattggtga ccaggcgcgc
      481 catctaagca ggctacggtg gcgcgggtgg ctccgtcaac gaatgggcga cggcattgcc
      541 agcctgcaag acggcacaaa gctaagcttt atttaggtcg aaggtaagcg ctatcgtgag
      601 ggagcgccct ttggcagaaa agggcgctca ccgaacgggg atccgcgtcc ccggttaatc
      661 gcccgatgac ttcttcatca tctccagcgc ctgatcgacc gagaagctcg ccgctttttg
      721 ccgtggcgga taatccttga acgatccgag gaatttgccc acgtaggcct gagccggaac
      781 cagcatgaag gcgtgatcca ggagccaatc gtaataggtg ttggatgtaa tgttggcgcg
      841 ttcataggga tcaagccgca gattatagat cagcgggatc cggagacgcg taaaaggctc
      901 ggcccagacc cggagcgttc ccggcgccct ttgctcggcg aatacgaact tccaattgtc
      961 gtagcgcagc gccgttagat caccatcatc cgagaaatag aagaactctt tgcgcggagt
     1021 cttgttctct gtcccggtca gatacggcac aaggttgtat ccgtcgagat gcaccttgta
     1081 ggtcatgctg ccggccttgt agccggtgag cagcttttcc ttgatgtcgg gcgcaccggc
     1141 ggcggcgagc agcgtcggca tccaatcgag atgcgagacg atctcattcg atacggttcc
     1201 cgctttgatc ttgcccggcc agcgaaccat ggccggaaca cggtacgcgc cttcccagtt
     1261 cgagttcttc tcattgcgga acggcgtcat ggcggcgtcg ggccatgaat tcatatgagg
     1321 accgttgtcg gtcgagtaca tgacgatggt attgtcctcg atgccgagtt ctttcagctt
     1381 cgcaaggaca gtccccacgt ttctgtcatg gtcgatcatc gtgtctgcgt aaggccccac
     1441 ccagcgcccc gcctggccaa ggctttccgg cttggtgtga gtgcggaaat gcatgtgggt
     1501 gaaattgacc cacaggaaca cgggcttgtt cgccttgacc tgcttgtcga gaaattcggc
     1561 ggagcgggca gcgatatcgt cgtcgattgt ctccatccgc tttttggtga gcggcccggt
     1621 atcttcaata cgttgggttc catctgagtt cgcgaagctg tggatcacgc cccttggccc
     1681 gaaattcttg cgaaaattcg ggaactcagg atcgtttgcc ttcggatagt ctgggagttc
     1741 cggctcttcc tcggcattca ggtgatagag attgccgtag aattcatcga agccgtgagc
     1801 ggtcggcagc atctcatcct tgtcgccgag atgattcttg ccgaactggc ccgttgcata
     1861 accctgagcc ttgagaagct ctgcgatagt cgggtcttcc ttccgaaggc cgaggtcagc
     1921 accgggcagg cccaccttgc tcagtccgct cctgaacacg ttctgtcctg tgatgaaggc
     1981 cgatcgtccc gcagtgcaac tttgctcgcc gtaataatcg gtgaagataa ccccctgttg
     2041 ggcgatggaa tcgatattgg gcgtcctgta gcccatcagg ccatttgtgt aggcgctaat
     2101 gttcgactgg ccgatgtcat cgccccagat gattacgatg ttgggctttt tctcatcagc
     2161 ggcgatcgcc ggcgatgctg cgacaagccc gatgactgcg agccatgcga gcaaaattcg
     2221 tcttatgctc atgcggtttc tccattaatc gacatcgttt tttttaagag tgtgatcgcg
     2281 agcatagtcc gacaacatcg cgatttggcc gatcggcgcg accggagaac ggcgcccaga
     2341 agtcccggat gaggcgcggt ggtgaacagg gcatggcggt cgttcatgtt caccaatggt
     2401 catccttaca gatatgcgaa tcgtccgggg gcgacgtatc gtaaatattt tcttttcttg
     2461 cttatgaaaa atactttaac agctacgtcg aagcttactt cattcacgtt taagtcactt
     2521 cagacgagcc atgccacgcc atgtttggtg gggttcttct acttaggggc ggtgagagcc
     2581 ttcggacact cgttcttgat gaagcgattc gcttcccgga gcatgtcgcc ctggaaggac
     2641 ccgaacctct tttccattcc ccaggcaacg aaaagcgttt tagcaatgaa aaacggaccg
     2701 tcatagtcgc tcccgctcag ggagatggct gtcacaacgc catccttgag ggtgaaatcc
     2761 gccgatacgt cggcggtgac atcatattct tcctctccga ccacgcacgc gaccgatttc
     2821 ccagtaagct tcaacgtatg ttcaggcgac gtgacagcgg cgtggatgtc ggcgcgtttg
     2881 gcgctgatct tgaggctgca cggcgccggc ccgagcgacc atgacaggtc cttcgactct
     2941 gccagagctc tgatttcttt tgcgtcccag cggtgagcaa gatcacagtt cacatccgga
     3001 ccgtttgcct cctttgtaag cacgatggag caaacatcct ttacgcactg cgcgttcttt
     3061 gccttctcct cggcggtatc gggcgcgagt gcaaggccgg acgacacatg acgcggtgcg
     3121 cggcttggcg cgagccatag cacagcgctt tccccgccaa ggcggcgatc gagaaaattg
     3181 taaggctcga atgtttggat ccaaacattc gccgaactcc ctgcagaggt gtccgtccta
     3241 tgtcgcactg gacttcggga gtcaattgct gaagtcgcgc ttccggggga tggcttaaag
     3301 accataggcc ggaagcgtca atgcgcacaa gaatgccaga cgtgcgccgg ctggcctaat
     3361 aagcgatctc gtagccttcc tcaaccgtct gaccttcgcg gggtagcggg cgaacgcgcg
     3421 cctccagatg cttg
//
LOCUS       DATMGX010000958         2344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_476390, whole genome shotgun sequence.
ACCESSION   DATMGX010000958 DATMGX010000000
VERSION     DATMGX010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476390"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            630..1931
                     /locus_tag="VNR65_15670"
     CDS             630..1931
                     /locus_tag="VNR65_15670"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWJ20156.1"
                     /translation="MSARTLMFCGVLAAILGLGVISIMSFAHVNEAHQPSVKAEAESD
                     GDHSRPKATPTAEPEEIPRLVKLSDDQIGASNIAVSPVRNGLIKQRLSVPGVIAMDSD
                     RVGRIAANVVGIVSELRKKLGDPVQKGEVIAVIESREVADARGEYISALVAYQLQSTL
                     FERDRALWEKQFTPEQQFLKTRSAYTEARVRVDLARQKLLALNVDSQEITETLSKQAS
                     AVVEAKEGAAAPYEGAAKSLRRYELRSPIAGRVVERRVDLGAPVGKEGQEAELYVIAD
                     LSRVWVELALPTADLENVKEGQSVLLKGSASHRKASGKIIFISPMLNNETRAARVIAA
                     IDNPDLAWRPGTFVTAEIATDEDAVDKSIPLAALQKFNGVNAVFVRTADGFERRDVLI
                     GKQDGDNAEVRMGLREDEQIAVSNTFILKAELGKSSAEESE"
     gene            1942..>2344
                     /locus_tag="VNR65_15675"
     CDS             1942..>2344
                     /locus_tag="VNR65_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWJ20157.1"
                     /translation="MIAHILSFSVKQRWLVLLLALFAVGFGLWSLSKLPIDAVPDITN
                     NQVQINTLAPALSPLDIEKQVTYPIETALAGAPGLKYTRSLSRNGYSQVTAVFSDKTN
                     VYFARQQILERLAEAKDAMPPGIEPKLGPVST"
BASE COUNT          506 a          717 c          656 g          465 t
ORIGIN      
        1 cacctgaccc aatgcggaag agaattcgga aaggcgatcc cagcggtggt gccggcttcg
       61 ccaccttcct cttgcctttt ctgctcaatt ccatatgtct gaatgcaggg atgaatcggg
      121 aacaaccaac acttggaagc tggcaagcga cttgatgacc caaccgcagc accaagatcg
      181 taagcggctt tctgttgtcg aagccggttc catcaggcat gtctgcctta tcctgattat
      241 actcgtgtcc ttccttgggc atacgtttgc ccacgctcac gtcgcccagc cctccctgcc
      301 gacagacccg tctcagctct cctccggggc gcccgataaa ggtgaaagcg cgcccgcgaa
      361 gcatcactcc atcagttgca gctgtttatc gacggcgttg ccttctaaaa gcggtgttga
      421 cttgccgcgg ttgagaaaat ctgcctgcaa gcaccgtttc gacagaggga ttcttccacg
      481 ctctgtcctg tcatttagcc ttgatcgccc gcctcggggc tgatctgcgc cgccagggac
      541 gcgccttctc gccgtccaga cgataagccg tcctgtgact actctaaaac gcgtgatcaa
      601 gggcaccaaa ggcccaggca ggaacaatca tgtctgcaag aactctcatg ttctgcggcg
      661 tcctcgcggc cattctaggc ttgggcgtca tctcgatcat gtctttcgct catgtgaacg
      721 aagctcacca accatcggtg aaggcagagg ccgaaagtga tggcgatcat agccgtccta
      781 aagcaacgcc aacggcggag cccgaagaaa tcccgcgcct ggtcaagctc agcgacgacc
      841 agataggtgc atccaacatc gccgtttctc ccgttcgaaa cggcctaatc aagcagcggc
      901 tgagcgttcc aggcgtcatc gcgatggatt ccgaccgggt tggccgcatc gccgccaacg
      961 tcgtcggcat cgtgtcggag cttcgcaaga agcttggcga tcccgtccaa aaaggtgagg
     1021 tgatcgccgt catcgaaagc cgagaggtcg cggatgcacg gggcgagtat atttctgctc
     1081 tcgtcgctta ccagctgcaa agcacgcttt tcgagcgcga tcgcgcattg tgggagaagc
     1141 aattcacccc agagcagcaa tttctcaaaa cgcgctccgc atatacggaa gcgcgagtga
     1201 gagtggacct cgccaggcaa aagctgctcg ccctcaacgt cgatagccag gagatcacag
     1261 aaacgctttc caaacaggcg tcggccgttg tcgaggccaa ggagggcgca gccgcgccat
     1321 atgaaggagc agccaagagc ttgcgccgct atgagcttcg ctcgccaatc gccggccgcg
     1381 tggtcgaacg cagggttgat ctgggcgcgc cggttggcaa ggaaggtcag gaggcggaac
     1441 tctatgtgat cgccgatttg tcgcgcgttt gggtcgagct cgcgttgcca acagccgatc
     1501 ttgaaaatgt gaaggaagga cagtctgtcc tcctcaaggg ctccgcgtcc caccgaaagg
     1561 ccagcggcaa aattatcttc atcagtccca tgctcaacaa tgagacccga gccgcccgcg
     1621 ttattgccgc gatcgacaac ccagaccttg cctggcgtcc tggcacattt gttacggctg
     1681 aaatcgcgac cgacgaagac gccgtcgaca aaagcatccc ccttgctgcc ctgcagaagt
     1741 tcaacggcgt caacgcagtt ttcgtccgca ccgcggacgg ttttgaacgg cgggacgtcc
     1801 ttatcggaaa gcaggatggt gacaacgctg aggtaaggat gggcctgagg gaggacgaac
     1861 aaattgcggt gtccaacacc ttcattttga aggccgaact tgggaagtcc tccgccgaag
     1921 agtccgagta ggaaaacaac aatgatagcg cacatcctct ccttctcggt gaagcagcgc
     1981 tggctcgtgc tgcttcttgc gctcttcgca gtcggcttcg gcctatggtc cttgagcaag
     2041 cttccgatcg atgccgttcc cgatatcacc aacaaccagg tgcagatcaa tacgcttgcg
     2101 ccggcgctct caccgctcga catcgagaag caggtgacct atcccatcga gaccgcgctt
     2161 gcgggcgcgc ccggcctcaa atacacgcgc tcgctgtccc gcaacggata ttcgcaggtt
     2221 acggcggttt tcagcgacaa gaccaatgtc tacttcgcgc gccagcaaat cctggagcgc
     2281 ctcgcggagg cgaaggacgc gatgccgccg ggcatcgagc cgaagcttgg cccggtttcg
     2341 accg
//
LOCUS       DATMGX010000959         6696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_35711, whole genome shotgun sequence.
ACCESSION   DATMGX010000959 DATMGX010000000
VERSION     DATMGX010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35711"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VNR65_15680"
     CDS             <1..504
                     /locus_tag="VNR65_15680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWJ20158.1"
                     /translation="LLVSEGFTTVEELAYVDEQEVASIEGFDENTAQEIQERAREFLE
                     RQEAELTEKRKALGVQDELGEVEGLTTAMLVTLGENGIKTVEDIADCATDELAGWQER
                     AHGQTKQHPGILQSYDLSKSDIEQIILNARIKAGWIEPPAAEEPSAEVLDPDEGGQDA
                     DSEEKAL"
     gene            476..1186
                     /locus_tag="VNR65_15685"
     CDS             476..1186
                     /locus_tag="VNR65_15685"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWJ20159.1"
                     /translation="MQIAKKKRSESEPILDFLDETAHLEEGDQGGNPIRTCIVDRERK
                     EKPSLLRFVLSPQEEVVPDVKGNLPGRGVWVTARNHIVADAVKRRAFQRAFRKQVTVS
                     DSLSLEVERLFKRSALERLSICNKAGLLVVGFAKVEEALKRHEIVALLHADTAATDGK
                     AKLDRKHEALFVGRDYIAPKNCFTSAEISLAAGSIGVIHAGLREGGATRAFLQALDRL
                     SAYCAGEAEIASLCQDRE"
     gene            1183..4074
                     /gene="infB"
                     /locus_tag="VNR65_15690"
     CDS             1183..4074
                     /gene="infB"
                     /locus_tag="VNR65_15690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWJ20160.1"
                     /translation="MSETKETDHKSEGGRKTMSLNVKRTVEQGHIRQNFSHGRSKSVL
                     VETKRKRPVSPSTAHEEPQQPKPAPQKPEPRAFQKPAPDQAGDKRPQKVLRQLSAGEV
                     DARARALLEARKREDIEKRERDIEDARLREEAQKQAIEDQKRRVEEEAARKVEEEAEK
                     LRAAEAAKVNEAEAAKAPKQEEVVARVVERPAAPPRRAGPDSVRVIPRPQSDDRRPQG
                     VPFRSGENGQRDGAREGTRPPGREGFRGDGPRPSGGAAGTARPSFGARPGPGAGSRPA
                     PTPALEVTRPERVREGDVALRARPVDAEEEDRRVKRGGLPGKAPERAAPAKKTVVDQR
                     LGRQRLTLSNALDEQQRERSLASLKRQRERQKLQAMGARQERVKIQRMVQLPEAITIQ
                     ELANRMAERAVDIIKFLMKQGQMLKINDVIDADTAELVAAEFGHTVKRVSEEDVEEGF
                     IGEVDADETLKSRAPVVTIMGHVDHGKTSLLDAIRKANVVAGEAGGITQHIGAYQVTT
                     PKGDPVTFIDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTVEAINHAKAAGVP
                     IIVAINKIDKPDADPLRVRTDLLSHDIVVESMGGDTLEVEVSAKQRLGLDRLLSAINL
                     QAEVLELMANPDRPAEGIVIEAKLERGRGPVGTVLVQRGTLKVGQLIVAGRAWGRVRA
                     LIDDKGENVVTAGPSTPVEVLGLDSAPEAGDQLAVVDNEARARELTEHRVRKLRDVRA
                     VGAGARTLEQMMKQAAKGGKKEFLLIIKGDVQGSVEAINAALEKLGTDEVEARIIHWG
                     VGGITSSDVALAEASGAVIIAFNVRADAQAKQQAERDGVEIRYYAIIYDLVDDVKKAM
                     SGLLSPVTREEFLGNAEILEVFNISKVGKVAGCRVTDGKVERGANVRLIRDNVVIHEG
                     KLSTLKRFKDEVREVVVGQECGMGFENYHDMKQGDVIECFRTHIETRSL"
     gene            4202..5374
                     /locus_tag="VNR65_15695"
     CDS             4202..5374
                     /locus_tag="VNR65_15695"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWJ20161.1"
                     /translation="MDDTSAALSVSVLKSVRDVCEDDWDRCANAPSWPYNPFLAHAFW
                     LALEESGSASPRKGWAPCHLILKDSGSTVAAIMPCYLKSHSYGEYVFDRSWAVAYERA
                     GGRYYPKLQTAVPFTPVSGRRMLIRPGVPEGKAHALLSAAAVDVAKQNRASSWHMTFA
                     TKEEWEALGALNFLQRTDTQYHWHNRGYGSFDDFLSALASRKRKTVRKEREQARASGV
                     TLEWLTGSDIKEAHWDAFFHFYMDTGNRKWGNPYLTRAFFSLVGQRMADRILLVMGRR
                     NGAYVAGALNFIGGDTLYGRYWGCLEDHIFLHFEACYYQAIDYAIANKLAHVEAGAQG
                     EHKLARGYAPETTYSLHHFADPGLAAAVASYLRQERQVVEREKQILSDALPYKKCG"
     regulatory      5449..5526
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            5663..>6696
                     /locus_tag="VNR65_15700"
     CDS             5663..>6696
                     /locus_tag="VNR65_15700"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HWJ20162.1"
                     /translation="MSLDCGRVLAPFEIAYQTYGTLNADKSNAVLVCHALTGDQHVAN
                     LHPMTGKPGWWETIIGPGKPVDTDQYFVICSNVIGGCMGSTGPASLDPATGKPYGLEF
                     PVITIDDMVRAQACLIDHLGIPDLFCVIGGSMGGMQVLEWCASFGDRVFSAVPVATAP
                     RHSSQNIAFHEVGRQAVMADPEWRGGRYLEEDTNPAKGLAVARMAAHITYLSDEALHR
                     KFGRNLQNREKITFGFDADFQVESYLRYQGISFVDRFDANSYLYITRAMDYFDLAADY
                     DGVLANAFRGTKTRFCLVSFTSDWLYPTRESRAVVQALNAVAANVSFVEIESDKGHDA
                     FLLHEPELFDT"
BASE COUNT         1462 a         1871 c         2103 g         1260 t
ORIGIN      
        1 ctgcttgttt ccgaaggttt caccaccgtt gaagaacttg cctatgtgga cgagcaggaa
       61 gtcgcgagca tcgaaggctt cgacgagaac acggcgcaag aaatccagga acgcgcccgc
      121 gaatttcttg agcggcagga agcggaactg accgagaaac gcaaggcgct cggcgttcag
      181 gacgagcttg gggaggtcga gggccttacg accgctatgc ttgttacgct cggtgaaaac
      241 gggatcaaga cggtagagga tattgccgat tgcgcaaccg acgaactggc cggatggcag
      301 gaacgcgcgc acgggcagac aaaacagcat cccggcattc ttcaaagcta cgatctcagc
      361 aaatcggata tcgagcagat catcctgaat gcccggatca aagccgggtg gatcgagccg
      421 cctgcagccg aggagccatc ggcggaagtg cttgatcccg atgaaggggg ccaagatgca
      481 gatagcgaag aaaaagcgct ctgagtctga gccgatactg gattttttgg acgagaccgc
      541 acatctagag gagggcgacc aaggcggcaa tcctatcaga acctgcatcg tggaccgcga
      601 gagaaaggaa aagccttctc tcctccggtt cgtgctatcg ccgcaggaag aagtggtgcc
      661 ggacgtcaaa ggcaatcttc cagggcgtgg tgtttgggta accgccagaa atcacatagt
      721 ggccgatgcg gtgaagcgcc gcgccttcca aagggcattt cgaaaacagg ttaccgttag
      781 cgatagcctt tctttagaag tggaacggct ttttaagcga tctgcactag aacggttatc
      841 gatttgtaat aaagcagggc tgttggtcgt agggtttgcg aaagttgagg aagcgctgaa
      901 gcggcatgaa atcgtggctc ttcttcatgc ggataccgcc gcaaccgatg gcaaagccaa
      961 gctcgatagg aagcacgaag ccttatttgt aggaagagat tatatcgcgc cgaaaaattg
     1021 cttcacgtct gccgaaatta gcttggcggc tggaagcata ggtgtaatac atgcgggact
     1081 tagagaaggc ggggcaaccc gcgcattctt gcaagctctc gatcgtttaa gcgcctattg
     1141 cgcaggggaa gcggagattg cttcgctgtg ccaggataga gaatgagcga aacgaaagaa
     1201 acggaccata aaagtgaggg cggccgcaag actatgtcgc tgaatgtgaa gcggacagtg
     1261 gaacaaggcc acatccggca aaacttcagc catgggcgct cgaaatccgt tcttgtcgag
     1321 accaagcgca agcgcccggt ttccccgagt acggcccacg aggagccgca gcagccgaag
     1381 cctgcgcctc agaagcccga gcctcgcgcc ttccagaaac ctgctcccga tcaggcagga
     1441 gataagcggc ctcagaaagt gctccggcag ctttcggcag gtgaggtgga cgctcgcgcc
     1501 cgcgctctcc tagaggcgcg caagcgggaa gacatcgaaa agcgtgagcg ggacatcgaa
     1561 gatgcccggc ttcgcgagga agcgcaaaag caggccattg aggatcaaaa gcgccgcgtt
     1621 gaggaagagg cggcgcgcaa agttgaggaa gaggctgaaa agctgcgcgc agccgaggcc
     1681 gccaaagtga acgaggccga ggctgctaaa gcgccgaaac aggaggaggt ggtcgcgcgt
     1741 gtcgtagaac gtccagccgc accaccgcgg cgagctggtc cggatagcgt tcgcgttatc
     1801 ccgcgtcctc aaagcgacga tcgcaggccg caaggcgtcc cattccgttc aggagaaaac
     1861 gggcagcgag atggagcccg cgaaggaacc cgtccgccgg gccgcgaagg atttcgaggc
     1921 gacggccctc gtccgagcgg cggcgcggcg ggaactgcgc ggccaagttt cggagcgaga
     1981 cctggacctg gcgcgggttc tcggcctgct cctacaccgg ctttggaagt cacacggccc
     2041 gaacgggttc gtgagggtga cgttgccctc cgcgctcgcc ccgtcgacgc cgaagaggaa
     2101 gatcgccgcg tgaagcgtgg cggcctgcca ggaaaggcgc cagaacgcgc agcgcctgcc
     2161 aagaaaacgg ttgtagacca gcgccttggc cgtcagcggt tgacactttc caacgcgctt
     2221 gacgagcagc agcgtgagcg cagccttgct tcactaaaac gccagcggga acgccaaaag
     2281 cttcaagcaa tgggcgctcg gcaggaacgg gtcaagattc agcgcatggt gcaattgcca
     2341 gaagccatca caatccaaga actcgccaac cgcatggccg agcgcgcggt cgacattatc
     2401 aagttcctga tgaaacaggg gcagatgttg aagatcaacg acgtaatcga tgctgacacg
     2461 gcggaactcg tcgcggccga attcgggcat acggttaagc gcgtttcgga agaggatgtc
     2521 gaagaaggct tcatcggcga ggtcgatgcc gatgagacac tgaaatcgcg cgcaccggtc
     2581 gtcacgatca tgggccacgt cgatcacggt aagacatcgc tgctcgacgc gatccggaag
     2641 gccaacgtgg tcgccggcga agctggcggt atcacgcagc atattggcgc ttatcaggtc
     2701 accacgccaa agggcgatcc ggtaactttt atcgatactc ctggccatgc cgcctttacg
     2761 gccatgcgtg ctcggggagc gaaagtgacg gatatcgtcg tgctcgtggt tgccgccgat
     2821 gacggcgtca tgccgcagac ggtggaagcg atcaatcacg ccaaagctgc cggtgtcccg
     2881 atcatcgttg cgatcaataa gatcgataag ccggatgccg accccttgcg cgtccggacc
     2941 gacttgctct cccacgatat cgttgttgaa agcatgggcg gcgacacgct agaggtcgag
     3001 gtctccgcga agcagcggct cggtctcgac aggctgctgt cggcgattaa tttgcaggct
     3061 gaagtacttg agctgatggc gaatccggac cggccagcgg aaggtatcgt catcgaggcg
     3121 aaactcgagc gcgggcgcgg tccggtcggc accgttctcg ttcagcgtgg aacgctgaag
     3181 gttggacagc tcatcgtggc aggccgcgcc tggggccgtg ttcgcgcctt gatcgacgac
     3241 aaaggcgaaa acgtagttac tgccggtcca tctacgcctg tggaggttct cggtctcgac
     3301 agtgctcccg aggcgggcga tcaactcgcc gtggtcgaca acgaagcccg ggcccgcgaa
     3361 cttaccgagc atcgtgtccg caagctgcgc gatgtgaggg ccgtcggggc cggagcacgc
     3421 acgcttgaac aaatgatgaa gcaagcggcg aagggcggga agaaggagtt cctgctcatc
     3481 atcaaaggcg acgttcaggg ctcagtcgaa gcgatcaatg cagcgctcga gaagcttggt
     3541 acggacgagg tcgaggcgcg gattattcac tggggcgtcg gtggaattac gtcctccgac
     3601 gtggctctgg ccgaggcttc cggcgccgtc atcatcgcct tcaacgttcg cgccgatgcg
     3661 caggccaagc agcaggcgga gcgtgatggc gtcgaaattc gctactatgc catcatctac
     3721 gacctcgtcg acgatgtaaa aaaggccatg tccgggcttc tctcgccggt cacgcgcgaa
     3781 gagttcctcg gaaatgccga gatcctcgaa gtgttcaaca tctcgaaggt cggcaaggtg
     3841 gcgggctgcc gcgtcaccga tggcaaagtg gagcgcggag cgaacgtgcg tctcatccgc
     3901 gacaacgtgg tgatccacga aggcaagctt tcaaccctca agcgcttcaa ggatgaggtc
     3961 cgtgaggttg ttgttggtca ggaatgcggt atgggctttg agaattacca cgatatgaag
     4021 cagggcgacg tcattgagtg cttccgcact catatcgaga cgcgcagcct ttaaaagggg
     4081 tgctcgcgct taggcgcggg tacaacccgg ctgacccaat cgttgacatt gacggtctgt
     4141 gcgccatttc ttgcgagggg cgagcgaagc cgcttcgtgc aattcagccg ggagccgaac
     4201 cttggacgac acaagcgccg ctttgagcgt gagcgttctg aagagtgtgc gcgatgtttg
     4261 tgaggatgac tgggaccgct gcgcgaacgc accgagttgg ccttacaatc cctttctggc
     4321 tcatgcgttt tggctcgcgc tagaggaaag tgggtcggcg tcgccgcgga aaggctgggc
     4381 cccctgccat ctcatcctga aggatagcgg cagcacggtc gccgccatca tgccgtgcta
     4441 cctcaaatct cattcttacg gcgaatatgt attcgaccgc tcctgggctg tcgcttatga
     4501 gcgagccggc ggccgctatt atcccaagct tcagaccgca gttccattca cgccggtttc
     4561 ggggcggcgc atgctcatca ggcctggcgt tcccgaaggc aaggcgcacg ccctgctctc
     4621 ggctgcggca gtggacgttg cgaagcaaaa ccgcgcctca tcctggcata tgaccttcgc
     4681 gacaaaagag gagtgggagg cgctcggcgc gctgaacttc ttgcagcgaa ccgacaccca
     4741 atatcactgg cataatcgcg gctatggcag cttcgacgat tttctgtccg cgctcgcatc
     4801 acggaagcgc aagacggtac ggaaggaacg cgagcaggcg cgcgcctcgg gcgtaaccct
     4861 tgaatggctg accggctcgg acatcaagga agcgcattgg gacgcctttt tccatttcta
     4921 catggatacc ggcaaccgga agtggggcaa cccttacctg acgcgcgcat ttttcagcct
     4981 tgttggccag cggatggccg atcgcatttt gctggtgatg ggaagacgca acggcgctta
     5041 cgttgcgggc gcgcttaatt tcatcggcgg cgatacgctt tatggccgtt actggggttg
     5101 cctcgaagac cacatcttcc tgcatttcga agcgtgctat taccaagcca tcgattacgc
     5161 catcgccaac aagctcgcgc atgtggaagc cggcgcgcag ggcgaacata aacttgcgcg
     5221 cggctacgcg cccgagacaa cttacagcct ccaccatttc gccgatcctg ggctcgcggc
     5281 ggcggtcgcg agttatctgc ggcaagagcg gcaggtggtc gaaagagaaa agcaaatttt
     5341 aagcgacgcc ttgccataca agaagtgtgg ctgagcaaag catcgaaggc agcgtgaagc
     5401 gcgagtgtgc cttctctctt gacgggaggc gcacgttctc ccttatagtt gcccaaccgt
     5461 ggtgatttgg ccggccggct tgcagccacg ttaaaaaact cgctaaaagg ccgcggctcg
     5521 ccagccccgg ctttcgatcg gccggttcgg acgtgtgtcc tggagggagg ctggccgcag
     5581 cttcatgaat aaaatgcttg tttccgcagg tgatctgcgg ggcttatctg acgatagtct
     5641 ggtctttcga agcgacgaag gaatgtcgct tgattgcggg cgcgtgcttg cgcccttcga
     5701 gattgcctac cagacctatg gcactctcaa cgccgacaaa tccaacgcgg tccttgtctg
     5761 ccatgctctg acaggggatc agcatgtcgc gaaccttcat ccgatgacgg gcaagcccgg
     5821 ctggtgggag acgattatcg ggccgggcaa gcctgtcgat acggaccagt actttgtcat
     5881 atgctcgaat gtcataggcg gctgcatggg gtctaccggg ccggcatcgc tcgatccggc
     5941 aaccggcaag ccctatggcc tcgaattccc cgtcatcacc atcgacgata tggtcagagc
     6001 gcaagcctgc cttatcgatc atcttggcat tcccgacctc ttctgcgtga ttggcggctc
     6061 gatgggcggc atgcaggtgc tggaatggtg cgcaagtttc ggcgaccgcg ttttctcggc
     6121 ggtgccggtc gcaaccgcgc cgcggcattc ttcgcaaaac atcgccttcc atgaagttgg
     6181 gcggcaggcg gtgatggcgg acccggaatg gcggggaggg cgctatctcg aggaagacac
     6241 gaaccccgcc aaaggcctgg cggtagcgcg catggccgcg catatcacct acctctccga
     6301 cgaggcactg catcggaaat tcgggcgcaa tctgcagaac cgggagaaga taacctttgg
     6361 tttcgacgcc gatttccagg tcgaaagcta tctgcgctac caggggatca gctttgtcga
     6421 ccgcttcgac gccaacagtt atctctacat cacgcgcgct atggattatt tcgaccttgc
     6481 tgccgattac gacggcgtgc tggcgaatgc tttcagaggc accaagacgc gtttctgcct
     6541 cgtgtcgttt acgagcgatt ggctctatcc cacgcgtgag agccgggccg tcgtccaggc
     6601 gctgaacgcg gtggctgcaa atgtcagttt cgtggaaatc gaaagcgaca agggccacga
     6661 cgcattcctt ttgcatgaac cggagttgtt cgacac
//
LOCUS       DATMGX010000960         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_10434, whole genome shotgun sequence.
ACCESSION   DATMGX010000960 DATMGX010000000
VERSION     DATMGX010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10434"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..>2188
                     /locus_tag="VNR65_15705"
     CDS             393..>2188
                     /locus_tag="VNR65_15705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWJ20163.1"
                     /translation="MVKRIRAHDWASSPMGSIEGWPPNLKGAVDLMLCSRHPAFIAFG
                     PELISLYNDAYIPLLGTKHPESLGQPYSDVWPELWPKFKPIIAATIAGEAQHIINHPV
                     ALAGRPDRPIGWFICSWSPLRDEAGTVKGFYCSATETTDQVLAQRALQDSEERLRFSL
                     KGAGAAAWQWDFLTNEMVWSPESYELHGRDPQFGKPSYEDWLHCLHPDDRPRVEKVVF
                     DAIEKRSQEYRTEYRVVFSSGEVRWLDALGKLEYAADGSPLRMSGINLDITARKRAEE
                     ALRESEERLRFCMKGAGAAAWHCDISTRQIVWSEECCELHGRDPKLGSPQYDDFLRYI
                     HPEDRDRVEKTNFDALLRGVPEIRMDYRVVLPTGEIRWLEALGKAEYATDGRPLRISG
                     ITLDITERTRAEEAQRQEHHELETILAAIPAAVMTTKDASCAEMIGNPAAYDLMERPA
                     GANLSKSAPAGQAPDNFEIFQKGCRLAPADLPIRKASACKCAVVGEELELRFVDGRSK
                     FLLGNALPLFDGAGDVRGAVAAFADITGLKSTEAALRESEERLKFALDAANAGTWELT
                     LRSGELVLSDRALALVDLPPGTPMTYQWAAAA"
BASE COUNT          446 a          621 c          670 g          451 t
ORIGIN      
        1 tgcccttcga ttggggctga aactgcttca cccgctttca tcttccggtt cctcttatgc
       61 agtgtcgacg cgaccggagc ggtgctcgcc agagattttc aggtttctgc ctgaatctaa
      121 gcaggctttt gtgacttccc aacgaatagg tggcatcgcg tagcctgtct accagaccag
      181 cgctcaattc gctctgcctt gagtggcaaa cttatgcacc ggttcgaagc gccgggccaa
      241 ttcggacata gagataaagt ggaaatccga acgcggcgtg ctatgcgaat gcccttctga
      301 gcggaaaaaa tcggcggagc gcggcgttga aagcgggatc atccttcgtg gctggcaacg
      361 ggtcaagata ctggccggga ttgtccggcg aaatggtcaa gcgtatccgc gcgcatgatt
      421 gggcctcgtc tcccatgggc tcgatcgagg gctggccgcc aaaccttaag ggcgccgtcg
      481 atctcatgct ttgcagccgg caccccgctt ttatcgcttt cggccccgaa cttatttctc
      541 tttataatga tgcctacatc ccgctccttg gaactaagca tcccgaaagc ctgggtcagc
      601 cttattcgga tgtgtggcct gagctttggc ccaagttcaa gccaatcatc gcggcgacaa
      661 ttgccggcga ggcgcagcat atcatcaacc atccggttgc cctcgccggc cgtccggatc
      721 gtcccatagg ctggttcatc tgttcgtggt cacctcttcg cgatgaggct ggtacggtga
      781 agggctttta ttgttccgcg accgaaacca cggaccaagt cctcgcgcaa agggcgctgc
      841 aggacagtga ggagcggctt cgtttctcac tgaaaggtgc tggcgcagcg gcttggcaat
      901 gggatttcct gacgaatgaa atggtgtggt cgccggaaag ctacgagttg cacggacgcg
      961 accctcagtt tggtaagccc agctacgaag attggcttca ctgcctgcat cccgatgatc
     1021 gtccgcgggt cgagaaggtg gtcttcgacg ccatcgagaa gcgatcgcag gagtatagga
     1081 cggaatatcg cgtcgtattt tcctccggcg aggtgcgctg gctcgatgcg ctagggaagc
     1141 tggagtacgc cgcagacggc tcacctttgc gcatgtcggg catcaacctc gacatcacgg
     1201 cgcgcaagcg ggccgaggaa gcgctgcgcg agagcgagga acggcttcgc ttctgcatga
     1261 aaggggcggg tgcggccgca tggcattgcg atatttccac gaggcaaatc gtctggtcgg
     1321 aagagtgctg cgagttgcac gggcgcgatc ccaaactcgg ctcgccacag tatgacgact
     1381 tccttcgata cattcatcca gaggatcgcg atagagtcga gaagacgaac ttcgatgccc
     1441 tcctgagggg cgtgcccgaa attcggatgg actatcgcgt ggtcctcccg acgggcgaaa
     1501 ttcgctggct cgaagcgctg ggcaaagcgg aatacgccac tgacggcagg ccgttgcgga
     1561 tatctggcat caccctggac atcacggagc gcacgcgggc ggaggaggcg caaagacaag
     1621 agcaccacga attggaaacc attctggccg cgatcccggc tgcggttatg accacgaaag
     1681 atgccagctg cgctgagatg atcggcaatc cggcagccta cgatcttatg gaacgccctg
     1741 cgggggccaa tctttcgaag tcagctcccg ctggacaggc ccctgacaac tttgaaatat
     1801 ttcaaaaagg atgccgcctg gcgccagcgg atttgccaat tcggaaagcc tccgcatgta
     1861 aatgcgcggt tgtcggagag gagttggagc ttcggttcgt cgatgggcgg agcaaattcc
     1921 tgcttggcaa cgcattgcct cttttcgacg gcgcgggcga tgtccgcgga gccgtcgccg
     1981 ccttcgcaga catcacaggc ctgaagagca cggaagccgc gctgcgcgaa agcgaggagc
     2041 gcctgaaatt tgcgctcgac gccgcgaacg cgggaacctg ggagctgact ctccgctcgg
     2101 gagaactcgt tttgtcggac cgggcgctgg ccttggtaga cctgcctcct ggaactccga
     2161 tgacttatca atgggcagcc gctgcaat
//
LOCUS       DATMGX010000961         3178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_340725, whole genome shotgun sequence.
ACCESSION   DATMGX010000961 DATMGX010000000
VERSION     DATMGX010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340725"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VNR65_15710"
     CDS             complement(<1..201)
                     /locus_tag="VNR65_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20164.1"
                     /translation="MSVDDLDLFCCPQTLPVPTATGISDLCDCYPVEIASHHEPAVGP
                     GGDILSIKALDRARLQIFQADLS"
     gene            587..997
                     /locus_tag="VNR65_15715"
     CDS             587..997
                     /locus_tag="VNR65_15715"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HWJ20165.1"
                     /translation="MTLLEWNENLSVGVSTIDDEHKNLVGMLNELYDAMQAKHSEEAL
                     GSVLDGLVAYTVYHFKHEEALFAKTGYAAAAEHKKEHDDLTKHVLDVQKEYQEGATAT
                     LSTEVLNFLRKWLLTHILVSDKKFGPHLNANGIK"
     gene            1498..2196
                     /locus_tag="VNR65_15720"
     CDS             1498..2196
                     /locus_tag="VNR65_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HWJ20166.1"
                     /translation="MRGLATVSILAASVAFCAQAQAELLVSRYAVSLGGIRMGEAILH
                     ATIDAKHYKVEITADVGTLLNNTKVQGEASGSRTGPKLTPERYKMVMSDGQESSVDFA
                     ASAAAGAKTANPLRGVLDPLSALLAASIRPASDTATPCDKVLTVLMSRAKIDASLHPA
                     EQQDPRLVSCRVHFAPNANSSANGPQLQRIQWEVNFQKLNKPQLWLVEQVSLPTDLGT
                     VTIDRVETAVSPGS"
     gene            2299..>3178
                     /locus_tag="VNR65_15725"
     CDS             2299..>3178
                     /locus_tag="VNR65_15725"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HWJ20167.1"
                     /translation="MTGQAITIPAEWEPQKAIWTAWPANSREWNGDLEAPRSDVAALV
                     HALASANKVRLLVHGAEAEASARAALGGGADIIQAAYGDIWLRDTGPIFARSPEGPVA
                     LRFRTNSWGGKFDLPDDATVGDDIARFSGSRIVRFDFVLEGGAIDHDGQGTILTTRQT
                     LLNPNRNGWTKEDAEKALAHAVGAKAVIWIDEGLANDHTDGHIDNIARFVAPGCVVCQ
                     SPAGPDDPNAQTLNAIAEALENATDAAGQKLDVVRIPGVGLYLNALGEVSPASYMNFI
                     IANGVVAVPVYGTGTEI"
BASE COUNT          709 a          893 c          941 g          635 t
ORIGIN      
        1 cgataaatcg gcctggaaaa tttgcagcct agcgcgatca agagccttga tgctcaatat
       61 gtcgccgcca ggacctaccg caggctcatg atggcttgcg atctcgacgg gatagcaatc
      121 gcataagtct gagatgccgg ttgccgtagg taccggcagc gtttgcggac aacagaagag
      181 atcgaggtca tcgactgaca ctctttgagg tctccccatg atgaaagcgc cggtgaggtc
      241 cggctagcga cgattgcgac acgggcaaag caatggtggt cgcacacagg atggtgagct
      301 ggcggccaca tgcgcacgtt tggaccggat agtcgattga ccttgtccaa ggctgatccg
      361 atcgccgaca gacggcagct ctgcggagca aaaaatccta ttcaatcaat aactgcgtcg
      421 cctcttaagc atcctgtcaa gcctctcgag tatgttgagc tggtatcgca gctaaggttt
      481 agcgcgaaga cgatcagtct tgctgatgtc agcaacaagt tgaacagaca ccttgtagga
      541 atcctgagag agctggtccc taaacaacta acaaaaagaa tattaaatga ctcttctgga
      601 atggaatgaa aatctctcag tcggcgtgtc cactattgac gatgaacaca agaatctcgt
      661 ggggatgctc aacgaactgt atgacgccat gcaagcgaag catagtgaag aggccctggg
      721 cagtgtgctt gacgggctgg tcgcctacac ggtgtaccat ttcaagcatg aggaagccct
      781 ctttgccaaa accggttacg ccgccgcggc agagcacaaa aaagagcacg acgacctgac
      841 caaacatgtt ctggatgtgc agaaggaata tcaagaaggc gcgactgcaa ccctgtcgac
      901 ggaagtgctg aacttcctga gaaaatggct gcttacccac attctggtca gcgacaagaa
      961 attcggcccg caccttaacg caaatgggat aaagtaagct cgggcagctg gctattgggg
     1021 tcgtgtcgcg actattcgct ggcgatgcct gagaaacttc ccagcgccat aaaggcaggt
     1081 cactggggcg agcacctttg ctaactgcgc ctcgcagtcc cgggatcaga tgcaccgatt
     1141 taggggggcg caggcgcgct catcgctcat ttcaaggcta acgaaatggc aagccgcacc
     1201 ctgtagaggt gcggctttga tttggggcat attagcaatt ggtttccaat gtatgagatc
     1261 acaacggagg agagtccggt ttattcccta actccatttt tggattggtt gctgaagcct
     1321 gagtagctag gttccaattt gttcatattt cgaacattaa tatatggaaa tagcagagcc
     1381 gaggcgtaac gcgcctcttt cagcgccgat ccgttgggtt gtaaccgtaa agtctgtcgg
     1441 atgagatcaa gaacgaaacg tggtttttcc atcccctctg ggggacgagc ctcggccttg
     1501 agagggcttg ccacagtctc gatcctggcc gcctcagtgg cgttttgcgc gcaggcgcaa
     1561 gctgagctgc ttgtttcgcg ctacgctgtg agcctcggcg gcattcgcat gggggaggcc
     1621 attcttcacg ccaccatcga tgccaagcac tacaaggtgg agatcacggc ggacgtgggc
     1681 acgcttctca acaacacgaa ggtccaggga gaagcgtccg gctcacgaac gggtccgaag
     1741 ctcacgccgg agcgttacaa gatggtcatg tccgatggac aggagagttc cgtcgatttc
     1801 gcagcttcgg ccgcagcggg cgcgaagacc gcgaacccct tgcgaggcgt tctcgatccg
     1861 ttgtcggcgc tgcttgcggc ttccatccgg cctgcgtcgg ataccgctac gccttgcgac
     1921 aaggtcctca ccgtgctgat gagccgcgcg aagatcgacg caagcctgca tcccgctgag
     1981 caacaagacc ccaggctcgt gagctgccgt gttcatttcg ccccgaacgc aaactcctcg
     2041 gcgaatgggc cgcagcttca aagaattcag tgggaagtga atttccagaa actcaacaag
     2101 cctcaactgt ggctcgtcga gcaggtgtcc ctccccaccg atctcggcac cgtcacgatc
     2161 gatcgcgtcg agaccgccgt ttcccccgga tcgtaaccgg ctccttgttg ctgcgcttga
     2221 agtcccggag cagtcgtata gatcattgac gcatggctcg ttcggagcga tgcgtcgctg
     2281 gtgaggatct atacaggcat gaccggtcag gcaatcacga taccggctga gtgggaaccg
     2341 caaaaggcaa tctggacggc atggcccgct aactcccgcg aatggaatgg cgatcttgag
     2401 gctccacgca gcgacgtggc cgccttggtg catgcgttgg cgagtgccaa caaagtccga
     2461 ttgcttgtgc atggcgcgga ggctgaggcg tcggcccgag ccgctctcgg cggcggcgca
     2521 gacatcatcc aggccgcata tggcgacatc tggctccgcg acaccggccc catttttgcc
     2581 cgctcgcccg aaggcccggt tgcgctgcgc ttcagaacca acagttgggg cgggaaattc
     2641 gaccttcccg acgacgccac ggttggcgat gacatcgctc gcttttcggg ctcgcgcatt
     2701 gtcaggttcg acttcgtgct ggaggggggc gctatcgacc atgacggcca aggcacgatc
     2761 ctcaccacaa ggcagacgct tctgaacccg aaccgcaacg gctggacgaa ggaagacgcg
     2821 gagaaggcgc ttgcgcatgc tgtcggcgcg aaggcggtga tctggatcga cgaagggctc
     2881 gcgaacgacc acacggacgg ccatatcgac aacatcgcgc gtttcgtggc gccgggctgc
     2941 gtggtgtgcc agtcaccggc cggacctgac gatcccaacg cgcaaacgct gaatgccatc
     3001 gccgaggcgc tggaaaacgc cacggatgca gcggggcaaa agctcgatgt cgtccgcatt
     3061 ccaggcgtcg gtctgtacct caacgctttg ggggaggttt caccggcctc gtacatgaat
     3121 ttcatcatcg cgaacggcgt cgtggccgtg cccgtttacg gcacagggac cgagatcg
//
LOCUS       DATMGX010000962         4883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_138424, whole genome shotgun sequence.
ACCESSION   DATMGX010000962 DATMGX010000000
VERSION     DATMGX010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138424"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VNR65_15730"
     CDS             complement(<1..652)
                     /locus_tag="VNR65_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20168.1"
                     /translation="MKLPSFMHAAPAASHWLSMLGLVDPANLHLATACVQVVVALAGA
                     AKAKAMRAAAAINRRMNGIPSESSGSATGWTKRCVVDPSRSQILVGLPATARAAVVLP
                     MSTKSRQTIYGGAILGAKIRAESARKRAAEAACEADRAEAEAWSIQMEGYGGPAQPSP
                     TIAQCLNGGYGWLEVECHRCKTRASIPLDAIRRPRGTPIWKLEAALKCRSCRKGRYA"
     gene            711..1376
                     /locus_tag="VNR65_15735"
     CDS             711..1376
                     /locus_tag="VNR65_15735"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HWJ20169.1"
                     /translation="MCNLYSITTNQAAIIALFRVINHNVGNLPPMPGVFPDYPAPVIR
                     NAGSDRKMVMMRWGMPPPPKLGGPPVTNIRNTSSPHWRAWLKPENRCLVPFNSFAEYA
                     PEPNPATKKKDVVWFALKDDRPLAAFAGIWTEFRGDRGTKSKPIPGPHLVYGFLTTAP
                     NAVAEPIHPKAMPVILTTDEERDVWMRAPWEEAKALQRPLPDDALKIVARGADKEDRA
                     AAA"
     gene            1394..1549
                     /locus_tag="VNR65_15740"
     CDS             1394..1549
                     /locus_tag="VNR65_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20170.1"
                     /translation="MNGNRYYGVRVEGAKYGVSFGSALAIAISSTANHSILWAIIHGI
                     LGWLYVI"
     gene            complement(1749..1943)
                     /locus_tag="VNR65_15745"
     CDS             complement(1749..1943)
                     /locus_tag="VNR65_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20171.1"
                     /translation="MAKFAIGEQVDNVADDHEGGTVVAVFPTVDGTFRYAVDTEGYGA
                     LQFFAEEKLIVHSCKAGMRP"
     gene            2636..2821
                     /locus_tag="VNR65_15750"
     CDS             2636..2821
                     /locus_tag="VNR65_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20172.1"
                     /translation="MSSSFHNADRRTYRKIMLVGLTFCALFVVVSFFAREQPANTYVL
                     KKADRLIRSAGEPLSAN"
     gene            complement(3001..3408)
                     /locus_tag="VNR65_15755"
     CDS             complement(3001..3408)
                     /locus_tag="VNR65_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HWJ20173.1"
                     /translation="MTSRRTAILVFTAAALLAGGFPAMAQSNHVSLIKRFDTDNDGTV
                     DLAEAKKAASELFDKLDRDRNVTLDKRELSRRLSAKELAAADPDHDGTLTKDEYLAVV
                     EQRFSAADADHDGKLDAKEFHSRAGLALARLLK"
     gene            complement(3776..3964)
                     /locus_tag="VNR65_15760"
     CDS             complement(3776..3964)
                     /locus_tag="VNR65_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20174.1"
                     /translation="MNNELINNEQLSDEQLLLVTGGEGKGDGLGRTRPDGNSTGHGKG
                     DGLGWLRDTLHNAFGINV"
     gene            4205..4435
                     /locus_tag="VNR65_15765"
     CDS             4205..4435
                     /locus_tag="VNR65_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20175.1"
                     /translation="MRYFFDIRDDFVPMSRDNVGRDFGYASDAIAHAKTLAEGLRAQG
                     MSIRSTARIYVISEIGSAVHEERVFLKALEVG"
     gene            complement(4624..>4883)
                     /locus_tag="VNR65_15770"
     CDS             complement(4624..>4883)
                     /locus_tag="VNR65_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ20176.1"
                     /translation="AAVTKNLGDKVEIRIRDNGTGIPNEVKEKIFNPFFTTKPSGEGT
                     GLGLSMSHDIIVKQHGGSIDVDTEPGLFTEFRIVLPRTSQR"
BASE COUNT         1022 a         1406 c         1397 g         1058 t
ORIGIN      
        1 gcgcgtatcg gcccttacgg cacgaccggc atttaagcgc ggcttctagt ttccagatag
       61 gcgtaccccg cggacgccgg atcgcatcga gcggaatgct ggcgcgagtc ttacagcgat
      121 gacattcaac ctcaagccag ccatagccgc catttagaca ctgcgcgata gttggcgatg
      181 gctgcgctgg tccaccatag ccctccatct ggattgacca cgcctcggcc tcggcacggt
      241 cggcctcaca ggcggcctcg gcagctcgtt tgcgggcgct ttcggcgcgt atcttcgccc
      301 cgaggatagc gccgccgtag atcgtctgac gcgatttggt gctcatagga agcactacag
      361 ccgcccttgc ggtggcaggc aatcccacta ggatttgaga tctggaagga tcgacgacac
      421 aacgctttgt ccagccggtg gccgagcccg acgactcaga ggggataccg ttcatgcgcc
      481 tgttgatagc tgctgccgcc ctcatagctt ttgccttcgc ggcaccggca agcgccacga
      541 cgacctgcac gcaagctgtt gcgagatgta agttggctgg gtccaccaaa cccaacatcg
      601 acagccaatg cgaggctgcg ggggccgcat gcatgaagga tggcaatttc atcggaccgg
      661 ttacccacac cccttggaaa aacctgcgaa aacagtgacg cagccgctca atgtgcaacc
      721 tctattcgat cacaacaaac caagctgcca tcatcgctct attccgggtt atcaaccaca
      781 atgtcggcaa cctgccgcct atgccgggtg tgttcccgga ttatcccgct ccggtgatcc
      841 gcaacgcggg gagcgaccgt aagatggtga tgatgcggtg gggcatgccg cccccgccga
      901 agctcggcgg tccgccggtc accaacatcc gcaacacctc ctcgccgcac tggcgggcct
      961 ggctgaagcc ggaaaaccgc tgcctggtcc catttaacag tttcgccgaa tatgcgccgg
     1021 agccgaatcc agcgaccaag aaaaaggatg tcgtctggtt cgcgctgaag gatgaccgac
     1081 cgctggcggc gtttgctggt atctggaccg agttcagggg tgaccggggc accaagtcga
     1141 agccgatccc ggggccacat ctggtctatg gcttcctgac gacggcaccg aacgccgtgg
     1201 ccgaaccgat ccatcccaag gcgatgccgg tgatcctgac gaccgacgaa gagcgcgacg
     1261 tttggatgcg cgcgccctgg gaagaggcga aggcgctgca gaggccgctt ccggacgacg
     1321 cgctcaagat cgtagcgcga ggcgccgaca aggaagatcg cgcggcggcg gcatgagcga
     1381 ctaagaggtg cacatgaacg ggaatcgtta ctatggtgtt cgagtcgagg gagcgaaata
     1441 cggcgtcagc tttggttcag cccttgctat cgcgatatcc tccaccgcca accactcgat
     1501 actttgggca atcatccacg gcattttggg ctggctgtat gtgatttagt tcgcactttt
     1561 tcggtagcga tagttggaca tgcgcgacca tgggccgggt ctcgaccatt atgcggataa
     1621 gtcgggtctc gcaaaaatcc gggaccgcct tcgggtgctt cgagcgcgtg atggtaccgg
     1681 cgcgcggggt acttccgtcc gcgccgccca tcagctctta tgtcgtcgcg acaaccgccg
     1741 gcgctaaatt acggtcgcat tccggccttg caggaatgaa cgattagttt ttcctcggcg
     1801 aaaaactgga gggcgccgta tccttccgtg tcgacggcgt atcgaaaggt gccgtcaact
     1861 gttggaaaca cggcgacaac tgtgccgccc tcatggtcgt cggctacatt gtcgacctgc
     1921 tcgccaatcg caaattttgc catcgaaacc ccctcgggaa tcattagtgc gattgtatcg
     1981 ccatgaatga gttaacgctt atgaactaac ggactcggat cagcatcccc gaatattgga
     2041 cgaccgctgt tgctataatg tctcagcgtt tacggttccg agacgcggtt ccatcatgga
     2101 caccccgcac ggcggagcct gaaaacaaaa aagccgtcgc cgggttgaga acatcggcga
     2161 cggcttttca aatgggcatg atacgcaacc cgcgatcaat gtggccgttt cttctgaaca
     2221 aatggttagc cgaatgggca gacgagcttg gggagcgccg atacccaaat gacacaggca
     2281 gctccgaatc gcacagctac cgaattcgac ctatgcgaga gcgaccgaat cattcgaggt
     2341 ttggaatggc gttccgcctt tggaacgctc ttcctcccga aacttcgccc gcccagtcgg
     2401 cgggctctct tttcgacggc gacgaaggcc gcgccaatgt ccgctttgga ccccactccg
     2461 gactcaactc ggacatcgcg gcatgtccgt tttgtgccat gaaccgacat ggccgatgca
     2521 atgctcgccc ggtcttatat cttgcagata ttatacgctc ttcgagtaac cttcataagc
     2581 aaggttaaat cgcgagtttg attgcgacgc gaggccgaag ctgtatggct ttggaatgtc
     2641 ctcgtcgttc cacaacgctg acagacgtac ctaccgcaag atcatgctgg tggggcttac
     2701 attttgcgct ctcttcgttg tggtcagctt ttttgcgagg gagcagcccg cgaataccta
     2761 cgttctgaaa aaggcggata ggctgatccg cagtgctggc gagccgttgt ccgccaatta
     2821 gttgctccca acaaaaagcc tcccgaaggc ggctcccaat tcaatcctga tgatcgcgga
     2881 tcaggcggcc ataaatgttg gctttgattt ccggcgataa gccatgaagc cgaggcccga
     2941 gaagcaagga tcataattgc ccatgtggag ggttcgggag aagcgatcat aagatcggcg
     3001 ctatttcaaa agtctggcga gagcgagccc ggctctggaa tgaaactcct tggcatccag
     3061 cttaccatca tgatcggcat cggcggcact gaaacgctgt tcgaccacgg cgagatattc
     3121 gtccttggtc agcgtgccat cgtgatcagg atcggctgcc gccagctcct tggccgacaa
     3181 ccgccgcgat agctcacgct tgtctagcgt gacattacgg tcgcggtcca acttatcaaa
     3241 cagctccgag gctgccttct tcgcttccgc aaggtcgacg gttccgtcgt tgtcggtatc
     3301 gaaccgcttg atcaagctga cgtgatttga ttgggccatg gctgggaaac caccggcgag
     3361 caaagccgcg gcggtaaaaa cgagaattgc ggtgcgacgt gaggtcatag gctcgacttt
     3421 ctcgattccg atttgcgacg atctgcagga gaactagtga ataacgcggg ggatgtaaag
     3481 ctcacgcttt gcgctcaccg atttcgcgct gcctagaagc gatccgagcg cgccatttcg
     3541 gtacggtccg ttcgggcaca tgggttacgg tttgatggcg cgacttccgg tatgggtcaa
     3601 tctcgaccga atccgaccct ccggcatatg tccggtttac ctcaggtagc gaccggatgg
     3661 cggacacgcc ggaacggccg cttcgtgcca acaggcgaat gttgcacgct gctgttgaag
     3721 tgaaagaggc cgccaactga ggcggcctct ttgtaacgtc agcaatgttc accgctcaga
     3781 cgttgatgcc gaaggcgttg tggagggtgt ctcggagcca gccaaggccg tcgcccttgc
     3841 catggccggt actattgccg tcggggcggg tgcgcccaag gccgtcgccc ttaccttctc
     3901 caccggtaac gaggagaagc tgttcatcgg aaagctgttc gttgttgatg agttcgttgt
     3961 tcatgctcgt ctccggctgc tcgaggatcg cttgtgatca gcagatacga tgtccacgag
     4021 accgatgcag tgattaacgt cacattgcaa acggcgtgct accgacgcga tgaggtaacc
     4081 aactgaggcg gccttacttc cctccgagtg gtttccccta gatcgatctg agaagttgat
     4141 caggatcaaa acgccattca acgggcccgt taatgttttg ccacttccat cataggacag
     4201 agtgatgcgc tatttcttcg atatcaggga tgactttgtg ccaatgtccc gagacaacgt
     4261 cggccgagat tttggttatg catcggatgc catcgcgcac gctaaaactc tcgctgaggg
     4321 cttgcgcgct caaggcatgt ccatccgatc aaccgctcgc atttacgtga tcagcgaaat
     4381 tggttcagcc gtccatgaag aacgcgtgtt cctcaaagcg ctagaggtcg gttgaggaaa
     4441 catccgcatt cattcgtccg gtccgggtca atcgcgtcac tttggctaca tgtcggtcac
     4501 ttccggtttt cacccaactc cggacatgcc gctgcgccgc aacaactgac gctatgtgcc
     4561 aacaggagac tcatgcactg cagcaaattg gcaatggtta gccggtgaaa tcggcgtggt
     4621 ggattacctt tggctggtcc gaggcaatac gatcctgaac tcggtgaaaa gtccgggctc
     4681 cgtatcgaca tcgatgctgc cgccgtgttg ctttacgatt atgtcgtggc tcatcgacag
     4741 gccaaggcca gtaccttcgc cagagggctt ggtcgtaaag aatggattga agatcttctc
     4801 tttgacctcg ttcggaatgc ccgtgccgtt atcgcggatg cggatttcaa ccttgtctcc
     4861 gaggttcttc gtcacagcgg caa
//
LOCUS       DATMGX010000963         2532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_400974, whole genome shotgun sequence.
ACCESSION   DATMGX010000963 DATMGX010000000
VERSION     DATMGX010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400974"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="VNR65_15775"
     CDS             complement(<1..1228)
                     /locus_tag="VNR65_15775"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWJ20177.1"
                     /translation="MIELPSISFASLDSKPNASTVIYFVAEGDGLPSHLKKLDGATVG
                     TISKAMEVAAFKRKRKSVVEILAPHGLSAGRIMLVGVGEPKTLNTREWMEIGGVVRGK
                     LPAKTKEADVSFDGVDSLNGEAPLSFALGFGLKNYAFKKYKSKGGSRGDGEDSEAENE
                     RTSPRLTVFSHSAEQLAGRFKKEQALLEGVYFARDLVNEPANILTPPEFVERLRALEG
                     EGLKVEVLDERALKSLGMNAMLAVGQGSAQPTAVVILRWNGVPQNASEGNLVFVGKGV
                     CFDSGGISIKPSQGMEDMKGDMAGAAAVAGVMTALARRKAPVCAIGLVGLVENMPSGT
                     ATRPGDVVTSASGQTIEILNTDAEGRLVLADLLWYAQTHLEPKLMITLATLTGAILVA
                     LGREYAGLFSNDDALAS"
     gene            1393..>2532
                     /gene="lptF"
                     /locus_tag="VNR65_15780"
     CDS             1393..>2532
                     /gene="lptF"
                     /locus_tag="VNR65_15780"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HWJ20178.1"
                     /translation="MLISRYIFRQTASAVLMILISLTLIVWLTSILKEIKLLTSEGQT
                     FILFLKITALALPNLMVTVAPIAFLIATLHSLNRLNGDSELIVLSAAGASVWRLFVPY
                     FALAVIISVSALAANVYVIPKAARLLNEYVSKVRADLLTQVMQPGEFTDLEPGLTFHM
                     RDKGKGGELLGVVVRDERDPKMATTVVAERGQVLAEGPRSSIELYDGQILRQEGGKQD
                     VQMVAFKTYAFDIGDMAPKQGPHDLGPRERDIGDLLYPDESTKFYQDNKHRFRSEIHE
                     RLSAAVYAIAFAIIATVYLGRPRTTREGRAGTMFTAFAIGAVVRLVGIAGINMVGKNL
                     SALWLIYGVPAVVSVLGLLMLRFNIAAPAISMPRLRLPWPLSLIRR"
BASE COUNT          528 a          743 c          711 g          550 t
ORIGIN      
        1 gggaggccaa ggcgtcgtcg ttcgaaaata gcccagcata ttccctccca agtgcaacga
       61 ggattgcgcc ggtaagggta gccagcgtga tcatgagctt cggctcaagg tgtgtttgcg
      121 cataccataa gaggtccgca aggacgagcc gtccttcggc atccgtattg agaatctcaa
      181 tggtttgccc cgatgccgat gtgacaacat cgccggggcg ggttgccgtt ccgctcggca
      241 tgttttcgac gaggccgaca aggccaatgg cgcatacggg cgctttgcgg cgagcgagcg
      301 cggtcatgac accggcaacc gcagcggcgc ccgccatgtc gcccttcatg tcttccatgc
      361 cttggctcgg cttgatggaa atgccgcccg aatcgaagca gacgcccttc cctacgaaga
      421 ccagatttcc ttcggatgcg ttttgcggga cgccgttcca ccgtaaaatg acaaccgccg
      481 taggctgggc actgccttgt ccgacggcaa gcatcgcgtt catgccgaga gatttgagcg
      541 cccgctcgtc gagaacctcg accttgaggc cctcgccttc aagcgcccga agccgctcga
      601 caaactccgg aggcgtcagg atgttcgctg gctcattaac aaggtcgcgc gcgaagtata
      661 cgccttccag gagagcctgc tctttcttaa aacggccagc gagttgctcc gccgaatgag
      721 aaaagaccgt cagcctgggc gaagtacgct cattttcggc ttcggagtct tcgccgtcgc
      781 ctcgcgaacc gcctttggac ttgtatttct tgaatgcata attcttcagg ccgaagccga
      841 gcgcgaaaga aagcggcgct tcgccgttca gcgaatcaac cccgtcgaaa ctgacatcgg
      901 cttctttcgt tttcgcgggc agcttgccgc gaacaacgcc cccgatttcc atccactcgc
      961 gtgtgttcag cgttttaggc tcgccgacac ccacaagcat gatgcggccc gcggaaagac
     1021 catgcggcgc cagaatctcg acgacgctct ttcgcttgcg cttgaaagcg gcaacctcca
     1081 tcgctttgga aatcgtccca accgtagcgc catccaactt cttgagatgt gacggcagcc
     1141 cgtcgccttc cgccacaaaa tagatgacgg tcgaagcgtt cggcttcgaa tccagggagg
     1201 cgaacgagat gctgggaagc tctatcatgg agaggataaa ccggtgtgca ggggggtgag
     1261 cgttaagacg aaaccctaca ggaaagcccc ataaatgcaa agcctgccat ggccgaaaat
     1321 gcgccatgaa gccatgcgta ttgcacactg aaattcgggg gaacccggca gactgccgcg
     1381 gagcgctaga acatgctgat cagccgctat atctttcgcc agacggcgtc ggcggtgctt
     1441 atgattctga tctcgctgac gcttattgtg tggctgacct caattctcaa ggagatcaag
     1501 ctcttaactt cggaagggca aacttttatc ttatttctta aaataacggc tttggcgctt
     1561 cccaatctca tggtgacggt ggcgccgatc gcctttctca ttgccacact tcactcgctc
     1621 aaccggctaa acggcgatag cgagctgatt gtgctgtccg ccgcaggcgc ttctgtctgg
     1681 cgcctgttcg taccatactt cgctcttgcc gttatcattt cagtcagtgc gctcgccgcg
     1741 aacgtctatg ttattcccaa ggctgcccgg ctgcttaacg agtatgtttc taaggtgcgg
     1801 gcggacctcc tgacacaggt tatgcaacct ggcgaattca ccgacctgga gcctggcctc
     1861 actttccaca tgcgcgacaa gggaaaagga ggagagttgc tcggcgtcgt cgttcgtgac
     1921 gagagagacc cgaaaatggc gacgaccgtc gttgcggaac ggggacaagt cctggcagaa
     1981 ggcccgcgct cctcgatcga gttgtacgat ggtcaaatcc tgcggcagga gggcggaaag
     2041 caggatgtgc aaatggttgc cttcaaaacc tatgcgttcg acatcggaga tatggcgccg
     2101 aaacaggggc cacacgattt aggtccgcga gagcgcgata tcggggatct tctctatccc
     2161 gatgaaagca caaaatttta tcaggacaac aagcaccgct tcaggtccga gattcacgaa
     2221 cgtttgtcgg ccgccgttta tgccattgcg tttgctatca ttgccacggt ctacctcggc
     2281 cggccgcgga cgacccgaga aggacgcgcc gggactatgt tcacagcttt cgcgatcggc
     2341 gccgtagtcc gcctggtcgg gattgccggc atcaacatgg tcggcaaaaa tctgtcggcg
     2401 ctttggctga tctacggcgt gcctgctgtc gtgagtgtgc ttggcctcct catgcttcgc
     2461 ttcaacatcg ccgcgcccgc aatctcaatg ccacggttac gcctgccttg gcctttaagc
     2521 ttgattcggc ga
//
LOCUS       DATMGX010000964         4796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_445247, whole genome shotgun sequence.
ACCESSION   DATMGX010000964 DATMGX010000000
VERSION     DATMGX010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445247"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VNR65_15785"
     CDS             <1..251
                     /locus_tag="VNR65_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20179.1"
                     /translation="YYFGPGIYRGIKRGAILAAPSDSQAVLARISVDQAVRALEKQEL
                     IRHLGVTIKVVDLKSLPEFDLNSSIAPAGFRPIFSVAP"
     gene            268..3774
                     /locus_tag="VNR65_15790"
     CDS             268..3774
                     /locus_tag="VNR65_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ20180.1"
                     /translation="MAKGGIWKSHFLLSTLVFVAGMVATICFSYFFYEKSEKDWNVRV
                     DQTAERLSSTLLAWLEESYAPVSGLVALVENSKTVESSEFLNAFESMQSRSTTVLLDE
                     ASLLRLNRIRQWQVMITSDTLGFPGRFITRNDVAETLSQASKRPNQFTLSPPFKSESG
                     RTISAIALSASSATEPAIVVGTLNYDTLLDGMRSGPVPKGIYPALSGRFLGQPDLKPV
                     VTLPGDRQFQRTSTTRVATAGADIEISWAATREFDGGPSYGVPLAGLLGGSGTTIVLT
                     LFIGSLIRRNKQIARKVDEATLALRQSSEALGRERERLQHILDASPVGVAITTDNIAR
                     FTNPRMEEVFALRVGSAVPDLYAHPEQRRAMLNELDRNGIVRDFAVQIRGANGEIRDC
                     LATLMHMEYEERQGVLGWIVDVTELTKIQTALSEAKVAAEDATKAKAEFLANMSHEIR
                     TPMNAVIGLAHLCLKTDLTSKQRDYVGKIHNAGTSLLSIINDILDFSKIEAGKLDVEN
                     VAFEVDSLMNNISTMVAQKIQDKGLELLFAISSDIPPTLLGDPLRLGQVLINLLGNAV
                     KFTETGEIRVVGELLERTGEKVKLRFSVRDSGIGMTKEQADRLFQAFSQADTSTSRKY
                     GGTGLGLAISKRLVELMGGSIWVDSEPSAGSTFSFTAWFGLSEASARRVVPLRLGSLK
                     TLVVDDNAAAREVLEEQLRSIGAEIEQVASGAEAILAVKQADADRPFDVLLVDWRMPG
                     LDGVETARRIRADSSLKSVPAIIIVTAFGREEVRSEAERVGVNGFLIKPVNQSTLVDA
                     LVEIFAPEHMAAVREAAEATAYDLNGLRVLLAEDNAINQQIAVELLEGVGATVDVANN
                     GREAVDKLLASGGDARYDLVLMDLQMPEMDGYQATARIRAEPRLADLPIIAMTAHAMA
                     EERDRCLAAGMRAHITKPIDPELLYRTLTQFYRPGHAAAVPARSVNHSPSPDVPLEID
                     GLDVEDGLRRVAGNMKLYRSLLGQFVEQQADTVSAVRAGLETQDFALVERLVHTLKGV
                     SANLGAKGVSRLSAELEGSLKNRDVGSLEAALSGMRVELARVIEAIRKSLTTRAAEAP
                     RRVSAMDLTETITLLKQIRQLLINDDGAALDFLLEARERIDGRISDTELNALQQAVSD
                     FDFAVALDCLTGIAQRNKLSLE"
     gene            3780..>4796
                     /locus_tag="VNR65_15795"
     CDS             3780..>4796
                     /locus_tag="VNR65_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWJ20181.1"
                     /translation="MPQLNRKTILIVDDTPTNIGIISSVLKDSFRTKVATNGEKALVL
                     ASADDKPDLILLDITMPGMDGYEVCRRLKTNPATRGIPVIFLTAKTEAEDEKQGFEVG
                     AVDYIHKPFSAPIVLARVKTHLALQTALQQARDSQRQADELLHCLLPQAAAEEIRNIG
                     TVIPRRYNDVAVLFCDVVNFTSYCDKHEPEDVVSRLDALFVAFERVAADHGLEKIKTI
                     GDAFMAAAGLLKKVGDPLESAVLCGLQMAATSIDAQLGWAVRVGVHIGPVVAGIVGQE
                     RYQFDIWGDTVNIAARMASKAMPGGVAVTDETWNQINDRFEGQSLGELDIKGKGTIRV
                     VAVQG"
BASE COUNT         1026 a         1356 c         1436 g          978 t
ORIGIN      
        1 catattactt cggcccgggt atctatcgtg gcatcaagcg aggggcgatt ctggcggctc
       61 cgagtgacag tcaggcagtg ctggcacgta tctcagttga tcaagcagtg cgcgcgcttg
      121 aaaaacaaga attgatccgc cacctcggag tcaccatcaa agtggtggat ctgaagtcgc
      181 tgccggaatt cgatctgaat tcgagtattg ccccggccgg gttccgtcca attttcagcg
      241 tcgcccctta ggctggactt taaagagatg gcaaagggcg ggatctggaa gtcgcatttt
      301 ctgctctcaa cgcttgtctt tgtcgctggc atggtggcga cgatttgctt cagctatttc
      361 ttctacgaaa agtcggagaa ggactggaac gttcgcgtcg accaaaccgc ggagcggctt
      421 tccagcacgc tgctggcctg gctggaggaa agctacgccc ctgtgtcggg actggtggcc
      481 ctggtcgaaa actccaagac agtggaatcg agcgaattcc tcaatgcgtt tgaaagtatg
      541 cagtcccggt ccaccaccgt gctgctcgac gaagcgagcc ttcttagact caatcgtatc
      601 cgccaatggc aggtgatgat tacatcggac acattgggtt ttcccggccg gtttatcaca
      661 cggaacgatg tcgccgagac cttgtcgcag gcatcaaagc ggccgaacca gttcacgctc
      721 agtccaccgt tcaaatccga aagcggccga accatttcag ctattgccct ttctgcttcc
      781 tccgcaactg agccggcaat cgtcgtcgga acattgaact acgatactct gctagacgga
      841 atgcgctctg gaccggtccc caagggcatc tatccggcgt tgagcggacg ctttcttggc
      901 cagcccgacc tgaaacccgt cgtcacgctc ccgggcgacc ggcagttcca gcgcacatca
      961 accacgcgag tggcgaccgc gggggccgat atcgaaattt catgggccgc gaccagggag
     1021 ttcgacggcg gcccgtccta cggagtgccc ctcgcaggat tgcttggcgg ctcggggacg
     1081 acaattgtac taaccttatt catcggctcc ctgatacggc gaaacaagca gatcgcgcgg
     1141 aaggtggacg aggcgacgct tgccctgcgg cagtcttccg aagccctggg cagggagcgg
     1201 gagaggcttc agcacatact cgacgcctcg ccggtaggag tggcgataac gaccgacaac
     1261 atcgcccgct tcaccaatcc gcgaatggag gaagtgttcg ccctcagggt gggatctgcc
     1321 gttcccgatc tatacgccca tcccgagcaa cgacgcgcca tgctgaatga gctcgatcgc
     1381 aatggtatcg tccgggactt tgccgttcaa attcgcggcg ccaatggaga gattcgcgac
     1441 tgcctcgcca cccttatgca tatggaatat gaggagcggc agggagtgct tggctggatc
     1501 gtagatgtaa ccgagttaac aaagatccaa acagccctgt cggaagccaa ggtcgccgct
     1561 gaagatgcca ccaaggcgaa ggctgagttc ctcgctaaca tgagccacga gatccgaact
     1621 ccgatgaacg cggtcatcgg cctcgcgcat ttgtgcctga agaccgacct tacttccaag
     1681 cagcgcgatt atgtaggcaa gattcacaat gccggcacct cgctgctgtc cattatcaac
     1741 gacatcctgg atttctccaa aatcgaggcg ggtaagctcg acgtcgagaa cgtcgccttc
     1801 gaagtcgact cgttgatgaa caacatttcg accatggtcg cgcagaagat ccaggacaag
     1861 ggcttggaac ttctgtttgc tatctcgtcg gacatcccac ccaccctgct cggcgatccg
     1921 ttgcgtttgg gacaggtgct catcaatttg ctcggaaacg ctgtgaaatt caccgagacc
     1981 ggcgagatcc gcgttgttgg cgaattgctg gagcggacgg gtgagaaggt caagctacgg
     2041 ttctccgtta gggatagcgg tatcggcatg accaaggaac aggcggaccg actgtttcaa
     2101 gctttctctc aagccgatac ttcgacgtcg cgcaagtatg gcggaaccgg actggggctg
     2161 gccatttcca aacgtctggt ggaattgatg ggcggctcga tttgggttga cagcgaaccg
     2221 agtgccggca gcactttcag tttcacagcc tggttcggtc tatccgaagc gagcgcgcgc
     2281 agggtcgtgc ccctgcggct gggatcgctc aagacgcttg tcgtggacga caatgccgct
     2341 gcccgggagg tgttggagga acaattaaga tccattggag cagaaataga acaggtggcg
     2401 tcgggtgcgg aagctatcct tgccgtcaag caggcagatg ccgaccgccc tttcgacgtg
     2461 cttttggtgg attggcgcat gcccggtctc gatggcgttg agaccgcgcg caggattcgc
     2521 gcagacagtt cgctcaagag cgtgccggcc attatcatcg tcaccgcctt cgggcgagag
     2581 gaggtccgca gcgaagccga acgcgtgggc gttaacggat ttcttataaa gccggtcaac
     2641 caatccacgc tggtcgatgc gctcgtcgaa attttcgcgc cagagcacat ggcggccgtt
     2701 cgcgaagccg cagaggccac ggcatatgac ctgaatggat tgcgtgtgct gctggccgag
     2761 gacaacgcga tcaaccaaca gattgccgtc gaactgctcg agggtgttgg cgccactgtc
     2821 gatgtcgcca ataacggaag agaggccgta gacaagctgt tggccagtgg cggcgacgcc
     2881 cggtatgacc tcgtgctaat ggacctgcaa atgccggaaa tggatggcta ccaggccacc
     2941 gcacgcatcc gcgccgaacc gcgactggcg gatctcccca tcatcgcgat gaccgcacac
     3001 gcaatggccg aagaacgcga ccgttgcctg gcggcgggca tgcgcgctca tatcaccaag
     3061 ccgatcgacc ccgaactgtt gtatcgcacc cttacgcaat tctaccggcc gggccatgca
     3121 gcggcggtac cagccaggtc ggtcaaccat tctccgtctc cggatgtgcc gctcgaaatc
     3181 gatgggctgg acgtcgagga tggattgaga cgcgtggctg gcaacatgaa gctttaccgc
     3241 tcgctgctgg gacagttcgt tgagcagcag gctgatactg tctctgcagt ccgtgccggc
     3301 ctggaaaccc aggacttcgc tttggttgaa cgcctggtac acacgctaaa gggcgtttcc
     3361 gccaatttgg gtgcgaaggg cgtcagcagg ctctcagccg aattggaagg atcgctcaag
     3421 aaccgcgatg tcgggtcgct cgaagctgca ttgtctggca tgcgcgtcga gttggcgcgg
     3481 gtcatcgagg cgatccgcaa atccctgaca actcgtgccg ctgaagcgcc tcggcgagtt
     3541 tccgcaatgg atttaacgga aactataacg ctgttgaaac agatccggca attgctgata
     3601 aacgacgatg gggccgcact ggacttcctc ctggaagcac gggagcgcat cgacggccgc
     3661 atttccgaca ccgaactgaa cgcgttgcag caggcggtgt ctgatttcga cttcgcagtt
     3721 gcactggact gcctcaccgg catcgcacag cgcaacaagc tttctctgga gtgagccgga
     3781 tgcctcaatt gaaccgcaag accatcctga tcgttgacga tacgccgacc aatatcggga
     3841 tcatatccag cgttctcaaa gactcgttca ggacgaaggt cgccaccaac ggagagaagg
     3901 ctctggtact cgcttcagcc gacgacaagc ccgatctgat ccttctggat attacgatgc
     3961 ctggcatgga cggctacgag gtgtgccggc ggctgaagac aaatccggcg acgcgcggaa
     4021 ttcccgtgat ctttctcaca gccaagaccg aggccgagga cgagaagcaa ggcttcgagg
     4081 ttggggcggt cgactacatc cacaagccgt tctcggctcc gatcgtgctg gctcgcgtca
     4141 agactcacct tgcgctacag acggcgctgc aacaggcgag ggattcgcag aggcaggctg
     4201 acgaactgct tcactgcttg ctccctcagg cggccgccga ggagattcgc aatatcggca
     4261 cggtcattcc gcgccgctac aacgatgttg cagtgctatt ctgcgatgtc gtcaatttta
     4321 catcgtattg cgacaagcac gaacccgagg acgtagtctc tcggctcgac gcattgttcg
     4381 ttgcgttcga gcgggtggcc gccgaccacg gtcttgaaaa aataaaaacc atcggcgatg
     4441 ccttcatggc ggccgcgggg ttacttaaga aggtgggtga cccgctggaa tcggcagtac
     4501 tttgcggcct ccagatggct gcaacgtcga ttgacgctca gcttggctgg gcggtgcggg
     4561 ttggtgtcca tatcggtccg gtcgtggcag gcattgtcgg gcaggagcgc taccagttcg
     4621 acatttgggg cgacaccgtc aatattgctg ccagaatggc cagtaaagca atgccagggg
     4681 gggtcgcggt gaccgatgaa acctggaatc aaatcaatga tcgttttgag ggccagtcgc
     4741 ttggcgaatt ggacatcaag ggcaagggaa ctattcgagt tgttgccgtt caggga
//
LOCUS       DATMGX010000965         4535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_456300, whole genome shotgun sequence.
ACCESSION   DATMGX010000965 DATMGX010000000
VERSION     DATMGX010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456300"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..2750)
                     /locus_tag="VNR65_15800"
     CDS             complement(165..2750)
                     /locus_tag="VNR65_15800"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HWJ20182.1"
                     /translation="MSSPEFLTQGQPSDFLREAILRHVRYTLVRPTSDLKPSDYLKPV
                     SLAIRDRIVDRMLETESRYRHKDSKKLYYLSMEFLMGRSLADNLSNLRLEELCREVLA
                     SFGVSLDEVLDSESDAGLGNGGLGRLAACFLESLATLGMPGYGYGIDYEYGMFKQEIF
                     NGFQREKPDRWKANGTPFEIEHPEEAVSIPLYGRVEAGSDPGENLKQTWLNPKIIVGI
                     PADMPVVGYLGHTVNWLRLFTASASEDFDIEIFNRGDYIRAVEQKIASENISSVLYPS
                     DSVMSGKELRLVQEYFLVACAFGDLMRRFRQQHKDIELLPSKVAVQMNDTHPSLAVAE
                     LMRVLLDDYQLEWDHAWDITERTLAYTNHTLLPEALEKWSVPLLEKVLPRHMLIVFNI
                     NHQFLREMQQYSFLDGEKLRKMSIIEEGYEKQVRMAHLCIVGSHSVNGVSRLHSDLIV
                     HSLVPEFAQIWPEKFNNKTNGVAPRRWLMEANPGLTDLISHTLDEHKWITDLNRLREL
                     EKFAEDADFREAFKEVKRQNKLKLSRVIQEQTGVVVDPDSMFDVQIKRIHEYKRQLLN
                     VMGIIHDYLGIVERGEIPGVPRTYVFAGKAAPGYWAAKQIIKLIHSVAEVVNHDEKVK
                     DSIKIAFLPDYRVSLAQLIMPAADLSEQISMAGMEASGTGNMKLAMNGALTIGTYDGA
                     NIEIAEEVGEDNIYIFGLRSEEIREMQKKGSYNPRDRYDNDASIREVMDALASDRFCP
                     NEHGLFRWIFDELVHRGDRYYHLADFPSYIETQQLISGEYRKEEIWWRKAVLNVARIA
                     KFSSDRTVTEYARDIWHIGPFEKSSRPAKMPVRPPAPAAKPIGDAVAVDEVAPDAEAK
                     HQVAD"
     gene            complement(3142..3339)
                     /locus_tag="VNR65_15805"
     CDS             complement(3142..3339)
                     /locus_tag="VNR65_15805"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HWJ20183.1"
                     /translation="MAHVQITMLDGRTTEQKRRAAKRMTEVLHEELNVNPEKLTIAFV
                     EVPRDSYASNGTLISDRDAPK"
     gene            complement(3446..3871)
                     /gene="ndk"
                     /locus_tag="VNR65_15810"
     CDS             complement(3446..3871)
                     /gene="ndk"
                     /locus_tag="VNR65_15810"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWJ20184.1"
                     /translation="MKTLQRTLSIVKPDAVERGATGDIIHTLLENKFRIIGMKMLHIT
                     KAQAEGFYAVHVHRPFFESLTDFMSSGPIVVMALEKEDAIADYRKLMGATDPAKADEG
                     TIRKKWAASIEKNAVHGSDADDTARFEISYFFAGYELAK"
     gene            complement(4151..>4535)
                     /locus_tag="VNR65_15815"
     CDS             complement(4151..>4535)
                     /locus_tag="VNR65_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWJ20185.1"
                     /translation="RGIGQSTALGIGGDPIIGTNFVDAISLFNDDPETEAIIMIGEIG
                     GTAEETAAEFIKKNVRKPVVGFIAGQTAPPGRRMGHAGAIISGGKGTAAEKIKAMEAA
                     GIHVAKSPAEIGETLVKAQAAAVNR"
BASE COUNT          839 a         1372 c         1306 g         1018 t
ORIGIN      
        1 gaaggatccg cactcaagac cggtcgcgcc agcgtccggt tttcttttgg ggcgcaaagc
       61 agccggatgc aggaagaacg ggcaagcgtc tacggaaacc actcctacac tgtcatcccg
      121 catctggagt gccgcacaac ccaaacgccg cttttttcag cctgctaatc cgccacctgg
      181 tgtttggctt cggcgtcggg cgcgacttcg tccacggcga cagcgtcgcc gatgggtttg
      241 gcagccggcg ccggcggacg gaccggcatt ttggccggcc ggcttgattt ctcgaacggg
      301 ccaatgtgcc agatgtcgcg cgcgtactcc gtgacggtgc ggtcgctgga gaactttgcg
      361 atgcgcgcca cgttcaggac ggccttgcgc caccagattt cttccttgcg atattcgccg
      421 ctgatgagct gctgggtctc gatgtaactg ggaaaatcgg caaggtggta gtagcggtcg
      481 ccgcgatgca cgagttcgtc gaagatccag cggaacagtc cgtgctcgtt aggacagaag
      541 cggtccgagg ccagcgcgtc cattacttct cggatgctgg cgtcgttgtc gtagcgatcg
      601 cgcggattgt acgagccctt cttctgcatc tcccggatct cttccgagcg caaaccgaag
      661 atatagatgt tgtcctcgcc aacttcttcc gcgatctcga tgttggctcc gtcgtaggtc
      721 ccgatggtaa gggcgccgtt catcgccagc ttcatgttgc cggtaccgga ggcttccata
      781 cctgccatcg aaatctgctc gctcaggtcg gcggcgggca tgattagctg cgccagcgac
      841 acgcggtagt cggggaggaa ggcgatcttg atgctgtcct tcaccttctc atcgtggttc
      901 accacctcgg ccacgctgtg gatgagcttg atgatctgct tggccgccca atatccggga
      961 gcggccttgc ccgcaaaaac gtaagtgcgc gggacccccg gtatctcgcc acgctccaca
     1021 atacccaagt agtcgtggat gatgcccatc acgttcagca gttgacgctt gtactcgtga
     1081 atgcgcttga tctggacatc gaacatagaa tccgggtcca ccacaacccc ggtctgctcc
     1141 tgaatgactc gggaaagctt cagcttgttc tgccgcttga cctccttgaa ggcctcgcgg
     1201 aaatccgcat cctcggcaaa cttttccagc tcacgcagcc ggttgagatc ggtaatccac
     1261 ttatgctcgt cgagcgtgtg gctgatcaaa tcggtgagtc cggggttggc ttccatcaac
     1321 cagcggcggg gcgcgacacc attcgtctta ttgttgaact tctccggcca aatttgtgca
     1381 aattcaggta ctaacgaatg gacaatcaga tcgctgtgca gcctggagac gccattgacc
     1441 gagtggctgc caacaatgca gaggtgggcc atgcgcactt gcttttcata gccttcctcg
     1501 atgatcgaca tcttgcgcaa cttctcgcca tccaggaagg aatactgctg catctcgcgc
     1561 aggaactgat ggttgatgtt gaagacgatc agcatgtgcc gcggcagcac cttctccagc
     1621 agcggcaccg accacttttc cagcgcctcg ggcagtagcg tgtgattggt gtaggccagg
     1681 gtgcgctcgg taatgtccca ggcgtggtcc cattccaact ggtagtcgtc gagcaaaacc
     1741 cgcatcagct cggcgaccgc caggctgggg tgggtgtcgt tcatctggac ggcgaccttg
     1801 gacggcagta actcgatgtc tttatgctgc tggcggaaac ggcgcatcaa gtcgccgaag
     1861 gcgcaggcga ccaggaagta ctcctgcacc aagcgcagtt ccttgccgct cattaccgag
     1921 tccgagggat acagcacact ggaaatattt tccgaggcaa tcttctgttc gacggcgcgg
     1981 atgtaatctc cgcgattgaa gatctcgatg tcgaaatcct ccgaagcgct ggcggtaaac
     2041 aaacgcagcc agttcaccgt atgtcccagg tagccgacaa cgggcatgtc cgcgggtata
     2101 cccacgataa tcttggggtt aagccatgtc tgcttcagat tctcgccggg atcgctcccg
     2161 gcttcaacgc gaccgtacag cggaatgctg accgcctctt cgggatgctc gatctcgaac
     2221 ggggtgccgt tggccttcca gcggtcaggt ttctcacgct ggaagccgtt gaagatctcc
     2281 tgcttgaaca tgccgtattc atagtcgata ccgtagccgt agccgggcat gcccaaggtg
     2341 gccagcgact ccagaaagca agctgccagc cggcccaagc ctccgttacc cagtccggcg
     2401 tcggactcgc tgtcgagcac ctcgtcaagg ctgacgccaa aactggccag cacctcgcgg
     2461 caaagttcct ccagccgcag gttcgacagg ttatcggcca gcgatcgccc catcaggaac
     2521 tccatcgaaa ggtagtacag cttcttggaa tccttgtggc ggtagcgcga ctcggtctcc
     2581 agcatccggt ccacgatgcg gtcgcgaatg gccagcgaca cgggcttcaa gtaatcggag
     2641 ggcttcaagt ctgacgtggg gcgaaccagg gtataacgaa cgtgccgtag gatggcctca
     2701 cgcaaaaaat cgctgggctg gccctgcgtg aggaattcgg gtgatgacat agaaaatggc
     2761 ggcctgtccg ctctacggtg actgtctccc ggagagtaat ccctctgcct tccgatagat
     2821 cgggcagcag cgtggttgcc ttcggggagt acgtcattgt cggcggacct cgccgagtct
     2881 gattcctcga cgaccgccgc ccaacccggc gcactctctt cctgccattc taatgcatga
     2941 ctctgccagc agaacaccag agttctacgg gtagaccctt cggacgtgac tgcgcaggtg
     3001 cggcttttcc acgcgacgct tctggcaaac ttagcggctg aagcggtttt cttcctgccc
     3061 ctagacgcga acctgaaggt ccgctctacc ctcaatgctc aagaggtggg aaaccttgca
     3121 ttggcgccgg ctgcgaccgt tttatttcgg agcgtcgcga tcgctgatca aagtgccgtt
     3181 gctggcatag ctgtctcgcg gcacttcaac gaatgcgatg gtcaacttct ccgggttgac
     3241 gttgagttcc tcgtgcaaga cctcagtcat gcgcttggcc gccctgcgtt tctgctcggt
     3301 ggtgcggcca tccagcattg tgatctgaac gtgcgccatg gctctccgat acgtccctcg
     3361 cggacctgcc cgccttggga tgcagcttgt cctgcggctc ggccggaacc cggctccggc
     3421 ccttgcccag aacggcccaa acaatttact tcgccaattc gtatccggcg aagaaataac
     3481 tgatctcgaa acgcgccgtg tcgtcggcat cggagccgtg caccgcgttc ttctcgatgc
     3541 tcgcagccca cttcttgcgg atggtgcctt cgtcggcctt ggccggatcg gtggcgccca
     3601 tcagtttgcg gtaatcggcg atggcgtctt ccttctccag cgccatcacg acgattgggc
     3661 cgctcgacat gaaatcggtg agcgactcga agaacggccg gtggacatgc acggcgtaga
     3721 agccttcggc ctgcgccttg gtgatgtgca gcatcttcat cccaataatg cggaacttgt
     3781 tttccaggag cgtgtggatg atgtcgccgg tggcgccgcg ctcgacggcg tcaggcttga
     3841 cgatggaaag agtgcgttgg agggttttca tgcttggatt gcgtcctctg ttctttggct
     3901 atccgttatg gcatttagca tttagctttt agccgttagc ttttagcaaa caaaattgtg
     3961 cattgtgatt ccgaccgagt ggcttcagcc acgagcggag gaatctgcgg ttcgtccgtt
     4021 gctgcgagca ggtcccctgg taccctcaac ttccatcgat ttgctaagag ctaacagctg
     4081 aaagctaaga gcctttgtct gccccggtgt tcaaacgatt tcgctgacgg atgatagctg
     4141 acagcttgct tcaccgattc accgccgccg cctgcgcctt caccagcgtc tcgccgatct
     4201 cggccgggga tttcgcaacg tgaatccccg cggcttccat ggccttgatc ttctccgcgg
     4261 cggttccctt cccgcccgag ataatggccc cggcgtggcc catgcggcgt cccggaggcg
     4321 cagtctgccc ggcgatgaag ccgactaccg gcttgcgtac gttcttcttg atgaactcgg
     4381 ccgcggtctc ttccgccgtc ccgccaattt cgccgatcat gatgatcgct tccgtctccg
     4441 gatcgtcgtt gaacaggctg atcgcgtcca cgaagtttgt cccgatgatc ggatcgccgc
     4501 cgataccgag tgccgtggac tgtccgatgc cccgc
//
LOCUS       DATMGX010000966         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_254369, whole genome shotgun sequence.
ACCESSION   DATMGX010000966 DATMGX010000000
VERSION     DATMGX010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254369"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VNR65_15820"
     CDS             <1..286
                     /locus_tag="VNR65_15820"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWJ20186.1"
                     /translation="LETRSKKNRSLAIANFLIGHMKQKMKFNSRPHRSANLRVLKATG
                     VPGVLIELGYLSNDEDEKLLTSSEWRAATAALLAENINAFMSERQAQIPL"
     gene            502..>2546
                     /locus_tag="VNR65_15825"
     CDS             502..>2546
                     /locus_tag="VNR65_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWJ20187.1"
                     /translation="MKPPTKKKKRRQSLFLSFLGFAFTAGVFLFLAAAAGAGYFLWVI
                     SKDLPDYDKLATYKPPVITRVHAGDGRLIAEFAKERRIYVPVQTMPKMLINAFLAAED
                     QSFYEHSGLDFRGILRAVYNNATKKKKEGASTITQQVAKNFLLTSEQSYLRKAKEAIL
                     AVRIERAYTKERILDLYLNQIYLGAGSYGVAAAGLRYFGKELHELKLHEAAYLASLPK
                     EPSKLQLTTARGRFFQEALNRRNATMDNMARFKFISVEEAENAKKMPLEMANRQVGPS
                     TFAAEYFAEEVRRSLIDLYGQEDNDNNSSYYAGGYSVRSTLDPNLQLMARAALRAGLV
                     AFDRKHGGWRGPVSKIDVAGGEWGAKLSEVPALSDVEPWRLGVVLQSAKDKAVLGLQP
                     ARYPNGIIDQERKTVELGQEGARWAIDGAARRGRIRGAPGVSDVLTIGDIVYVSPPTE
                     LDKENNPPKPVKGEVTAGAPRWQLMQVPQIGGATVVMDPHTGRILALVGGFSFAESQF
                     DRALQARRQPGSSFKPIVYAAALDNGYTPASVVNDAAIEIDQGNGLGVWKPDNYDRQV
                     HGAQPLRIGIEKSRNLMTVRLAQDIGMPTVSEYARRFGVYDDLPPLLSMALGAGETTL
                     VRLTTAYCMFVNGGKKINYTFIDRIQDRLGQTIWRHDTRQCKGCIATAWKGQDEPEL"
BASE COUNT          578 a          682 c          741 g          545 t
ORIGIN      
        1 gcttgagacc cggagcaaaa agaacaggtc actcgcgatc gcgaattttc tgattgggca
       61 catgaagcag aaaatgaagt tcaacagcag gccccaccgt tcggccaatt tgcgcgtgct
      121 aaaggctacg ggcgtgccgg gcgttttgat tgagcttggt tatttgagca atgatgagga
      181 cgagaagctt ttgacatcga gcgaatggcg ggcagcgacc gctgcccttt tggcggaaaa
      241 tattaatgca tttatgtccg agcgtcaggc tcaaatcccg ctgtaaatca tttactaaaa
      301 aaatggattg catcaactga cgcttgttca ggatatgatc ttgtggttag ccagcttgca
      361 gcctgctatt tgctgtcaca atttagctat gcgattatgt tcgtgattgc cccgctagaa
      421 tccctccggt cgctcaattt ggttttcatc gcgaaaccgg ccgaataacc gaacgtgaaa
      481 cttatgcaaa gtaatagacc tgtgaaaccg cctactaaga agaagaagcg ccgccagagc
      541 ctgtttttga gttttcttgg ctttgctttt accgctggcg ttttcttatt cctagctgcc
      601 gcggccggtg ctggttattt tctttgggtt atttcgaaag acttgccgga ctatgataag
      661 ttggcgacct acaagccccc tgtcatcacg cgagtccatg ccggcgacgg caggctcatc
      721 gcagagtttg cgaaagagcg gcgcatttac gttccggttc agaccatgcc gaaaatgctc
      781 atcaacgcgt ttctcgccgc cgaagaccag agtttctatg agcacagcgg gcttgacttt
      841 cgtggcatcc ttcgcgccgt ctacaacaat gcgaccaaga aaaagaagga aggcgcctcg
      901 accatcacgc agcaggtcgc gaagaacttt cttctgacga gcgagcaatc ctatttgcgc
      961 aaggcaaaag aggcgatact cgcggtcagg atcgagcgcg cctatacaaa ggaacgcatt
     1021 ctcgacctct atctcaatca gatctatctg ggcgctggat catatggcgt ggcagctgcc
     1081 ggattgcgct atttcggcaa ggagcttcat gagttgaagc tccatgaggc ggcatatctg
     1141 gcttccttgc cgaaggagcc ttccaagttg caactcacga cggcgcgggg cagatttttt
     1201 caggaggcgc tcaaccgacg gaacgccacc atggacaaca tggcgcgctt taaattcatc
     1261 agtgtggaag aggccgagaa cgcaaagaag atgccgctcg agatggcgaa ccggcaggtt
     1321 ggtccgtcta ccttcgcggc cgaatatttc gccgaggaag tccggcggtc gcttatcgat
     1381 ctttacgggc aggaggacaa cgacaacaac tccagctatt atgcgggcgg ctattccgtt
     1441 cggtcgaccc tcgatcccaa tctgcagttg atggcgcgcg ccgcccttcg cgcgggcctt
     1501 gttgcttttg acaggaagca cggcggctgg cgcggtccag tctccaagat cgacgtcgcc
     1561 ggtggcgaat ggggcgcaaa actttccgag gtgcctgctc tttctgatgt cgaaccgtgg
     1621 agactcgggg ttgtgttgca gtcggcgaaa gacaaggctg tcctcggtct tcagccggcc
     1681 cgttatccga acggcataat cgaccaggag aggaagacgg tcgaactggg tcaggaaggg
     1741 gccaggtggg ccatcgacgg cgcggcccgc agagggcgga tcaggggcgc tccaggcgtg
     1801 agcgacgtcc tgacgattgg cgatattgtc tatgtttcgc ccccaaccga actcgacaaa
     1861 gagaataatc cgccaaagcc agtcaaaggc gaggtgactg ctggcgcgcc gagatggcaa
     1921 ttgatgcagg tgccgcagat cggcggcgcg acggttgtca tggaccccca cacgggacgg
     1981 attttggcgc tcgtcggcgg gttcagtttc gcggagagcc agtttgacag agctctccag
     2041 gcaagacgcc agcctggttc gtcattcaag ccgatcgttt acgcagccgc cctcgacaac
     2101 ggctatacac cggccagcgt cgtgaacgac gccgcgatcg agatcgatca aggaaatggc
     2161 ctgggcgtct ggaagccgga caactatgac agacaggtgc acggtgcgca gcccttgcgc
     2221 atcggcatcg agaaatcccg aaacctgatg acggttcgac ttgcgcaaga tatcggcatg
     2281 ccgaccgtaa gcgaatacgc ccgccgtttc ggcgtctatg atgatctgcc gccgctgctc
     2341 tcaatggcac tgggcgcagg tgaaacaacg cttgtgagat tgacgacggc ctactgcatg
     2401 tttgtcaatg gcggcaagaa gatcaactac acctttatcg accggatcca ggatcgtctc
     2461 gggcagacca tctggcggca cgatacgcgc caatgcaaag gttgcatcgc gacggcctgg
     2521 aaagggcagg atgagcctga gcttca
//
LOCUS       DATMGX010000967         3401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_209061, whole genome shotgun sequence.
ACCESSION   DATMGX010000967 DATMGX010000000
VERSION     DATMGX010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209061"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1084
                     /locus_tag="VNR65_15830"
     CDS             38..1084
                     /locus_tag="VNR65_15830"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWJ20188.1"
                     /translation="MSKSRDGNFFEDFKLGQLIEHSTPRTVTVGDVSLYTALYGARFA
                     VQSSDAFAKAVGYPRSPIDDVLVFHIVFGKTVPDVSLNAVANLGYAECRFLKPVYPGD
                     TLSATSEVIGLRENSNKQSGVVYVRSTGRNQDGAPVLEFVRWVMVRKRNERAPGPEEY
                     VPQLKKVVDAKALGAALPPLDIRAYSDALSGSARRWADYEVGEKVDHLDGVTIEEAEH
                     QLAARLYQNNARVHFNQHEQGQGRFGRRLVYGGHIISLARALSFNGLENAFHVAGINA
                     GRHVSPAFAGDTVYAWSEVLEKVPLDRKDAGALRLRLVALKDKAAASFALKAGDQYDP
                     AVLLDLDLWVLMPL"
     gene            1401..1829
                     /gene="sdhC"
                     /locus_tag="VNR65_15835"
     CDS             1401..1829
                     /gene="sdhC"
                     /locus_tag="VNR65_15835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWJ20189.1"
                     /translation="MMAEAKPTAGARTMAPKPMSPHLSIFRLYVNMVLSIMHRITGVV
                     NYWGSILLTAWLISAAMGEPQFNAVNSFMGSPIGLIILVGFTWSVMHHMLGGIRHFIW
                     DTGHGFSLPVVRALSWATLFGSVTLTGLIWFVGLSLWGNR"
     gene            1829..2218
                     /gene="sdhD"
                     /locus_tag="VNR65_15840"
     CDS             1829..2218
                     /gene="sdhD"
                     /locus_tag="VNR65_15840"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HWJ20190.1"
                     /translation="MAKTYRTQLKKVRGLGSAIGGPGTDHFWMQRLTAVSNIFGAAFV
                     VYAGLSLVGASRTSVQLFFSNPLHAVLALLLVISITFHMRLGMQVIAEEYAPHWLKVP
                     CLFLNTFWTFSVAVACTLAIIKLFLGM"
     gene            2222..>3401
                     /locus_tag="VNR65_15845"
     CDS             2222..>3401
                     /locus_tag="VNR65_15845"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWJ20191.1"
                     /translation="MPDATKLNRNGKNGGAPKVNGRAYPVEDHTFDVVVVGAGGSGLR
                     ATLGCAEAGLKTACVTKVFPTRSHTVAAQGGIAAALGNMGRDNWRWHMYDTVKGSDWL
                     GDQDAIEYLCRNAPAAVYELEHYGMPFSRTEDGKIYQRPFGGMTTDFGDGPPALRTCA
                     AADRTGHAMLHTLYGQSLRHSAEFFIEHFALDLIMDDGICRGVMTWSLEDGSIHRIRA
                     KKTIIATGGYGRTYFSCTSAHTCTGDGNAMVIRAGLPLQDMEFIQFHPTGVYGAGVLI
                     TEGARGEGGYLTNSEGERFMERYAPTAKDLASRDVVSRAMTIEIREGRGVGPKKDHIY
                     LHLDHLDPKMLAERLPGITESAKIFAGVDLRRQPIPVIPTAHYNMGGIPTNHYGEAIS
                     P"
BASE COUNT          632 a         1023 c         1021 g          725 t
ORIGIN      
        1 tagctgtgct caacctggac agtagagcga gggcagggtg agtaaatcgc gcgacggcaa
       61 ttttttcgag gacttcaagc tcgggcagct gattgagcac tcgacgccgc gtacggtcac
      121 ggtgggtgat gtgtcgcttt acacagcgct ttacggcgcg cgttttgcgg ttcagtcttc
      181 cgacgccttc gcgaaggccg ttggctatcc gcgaagccct atcgacgatg tgcttgtctt
      241 ccacatcgtg ttcggcaaga cggtgccgga tgtctctttg aatgccgtgg ccaacctcgg
      301 ttacgccgaa tgccgcttcc tgaagcccgt ctatcccggc gacacgctct ctgccacatc
      361 cgaagtcatc gggctgcgcg aaaactccaa caagcagagc ggcgtcgtct atgtccgctc
      421 gaccggccgc aatcaagacg gcgctcccgt gctcgaattt gttcgctggg tcatggtgag
      481 aaagcgaaac gagcgcgcgc ctggtccaga ggagtatgtg ccccaactga agaaagtggt
      541 cgatgcaaag gcgcttggag ccgcgctccc gccgctggac ataagagctt acagcgacgc
      601 gctttccggc agtgcgcgcc ggtgggcgga ttacgaggtt ggcgagaagg ttgatcacct
      661 cgacggcgtg accatcgaag aagccgagca tcagcttgcg gctcgcctct accagaacaa
      721 cgcgcgggtt cactttaacc agcatgaaca ggggcagggc cgctttggcc gccgcctggt
      781 ttatggcggt cacatcatct ctctcgctcg ggcgctcagt ttcaacggcc tcgaaaatgc
      841 cttccatgtc gccggcatca atgcgggccg gcacgtgtct cccgctttcg ccggcgatac
      901 ggtctatgcc tggagcgaag tcctggaaaa agtgcccttg gatcggaagg acgcgggcgc
      961 gttaaggctg aggcttgttg cgctgaagga caaggccgcc gcgagcttcg cgcttaaggc
     1021 aggcgatcaa tacgaccccg ctgtgctcct cgatctcgat ctctgggtgc tgatgccact
     1081 ttagtcttgc gtggctcgtt gccgccatgg ctggcatgcc gaattcgcta aaattgtgca
     1141 gtgcatcata aacccttgat cgccttggga aagcacgaaa tgccgagacg cggcagcgct
     1201 cctgttctcc gttataacca agtagtagaa tggtttggtt ggttgcggcc cactagactc
     1261 gcttcagccg catcgtttac gcttttcgcg caagtccaga cgctaacggt tgttcagcag
     1321 gatgtttgtt gccccctttg cggcatcgca gtaggaacct tgcattaaga aaaaacgcgc
     1381 gccgcgttaa gaaggattgc atgatggcgg aagcaaagcc gaccgcggga gcccggacca
     1441 tggccccgaa gccgatgtcc ccgcaccttt ccatcttccg cctctatgtg aacatggtgc
     1501 tgtcgatcat gcaccggatc acgggcgtcg ttaactattg gggcagcatc ctcttaacgg
     1561 cctggcttat ctctgctgcg atgggtgagc cgcagttcaa cgccgtcaac tcgttcatgg
     1621 gatcgccgat cggccttatt atccttgtgg gtttcacttg gtcggtcatg caccatatgc
     1681 tcgggggtat caggcatttc atctgggaca cgggccatgg cttctctttg cccgtcgttc
     1741 gcgctctgtc ctgggcgaca ctcttcggat ctgtgacgct aaccgggctg atatggtttg
     1801 tggggctgtc tttgtggggg aaccgctgat ggcgaagact tacagaacgc agcttaagaa
     1861 ggtccgcggg ctcgggtcgg ccattggcgg acccggcacc gatcacttct ggatgcagcg
     1921 ccttaccgcg gtttcgaata ttttcggcgc ggcgtttgtc gtttacgcag ggctttcgct
     1981 cgtcggcgct tcgcgcacca gcgtccagct cttcttctcc aatccgcttc acgctgttct
     2041 ggcgctactg ctcgtcatat caataacctt tcacatgcgg ctcggcatgc aggtcatcgc
     2101 cgaggaatat gcgccgcatt ggctgaaggt gccttgtctg ttcctgaata ccttctggac
     2161 gttctccgta gccgttgcct gcacgctcgc gatcatcaag ctttttctgg gaatgtaact
     2221 catgcctgat gcaaccaagt tgaaccgcaa cggcaagaac ggcggggcgc ccaaggtgaa
     2281 tgggcgcgcc tacccggtgg aggatcacac cttcgatgtc gtggttgtgg gggcgggcgg
     2341 ctccgggctt cgcgcgacgc tgggctgcgc cgaggccggg ctcaagacgg cttgcgtcac
     2401 caaggtgttt ccgacccgtt cgcacacggt ggctgcgcaa ggcggcatcg cagcagcgct
     2461 tggcaatatg ggccgggaca actggcgctg gcacatgtac gacaccgtga aagggtccga
     2521 ctggctcggc gaccaggacg ccatcgagta tttgtgccgc aacgctcccg ctgcggtcta
     2581 cgagcttgag cattacggca tgccgtttag ccgcaccgag gacggaaaaa tctaccagcg
     2641 accctttggc ggcatgacga ctgatttcgg ggatggccct ccggcgctca ggacttgcgc
     2701 cgcggcggac cgcactggcc atgccatgtt gcacacgctc tatgggcaat cgctgcgcca
     2761 ttccgccgag ttcttcatcg agcacttcgc gctcgacctc atcatggatg atggcatctg
     2821 tcgcggcgtc atgacttgga gcctcgagga cggcagcatt caccgtatcc gcgccaagaa
     2881 gacgatcatc gccacgggcg gctatggccg cacctacttc tcgtgcacca gcgctcacac
     2941 ctgcaccggc gacggcaacg ccatggtcat tcgcgccggc ctgccgcttc aggacatgga
     3001 attcattcaa ttccatccga caggggttta cggcgctggc gtgctgatca cggagggcgc
     3061 acgcggcgaa ggcggatacc tcaccaactc cgaaggcgag cgtttcatgg agcgctacgc
     3121 acccaccgcc aaggatctcg ccagccggga cgtggtctcg cgggccatga caatcgaaat
     3181 tcgcgagggg cggggcgtcg ggccgaagaa ggatcacatc tatcttcatc tcgaccatct
     3241 ggacccgaaa atgctggccg aacgcctgcc gggcatcacg gaaagcgcga agattttcgc
     3301 gggcgtcgat cttaggcgcc agccgatccc ggttatcccg acggcacact acaatatggg
     3361 cggcattccc acgaaccatt acggggaggc catctcccct c
//
LOCUS       DATMGX010000968         5142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_10683, whole genome shotgun sequence.
ACCESSION   DATMGX010000968 DATMGX010000000
VERSION     DATMGX010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10683"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..749)
                     /locus_tag="VNR65_15850"
     CDS             complement(30..749)
                     /locus_tag="VNR65_15850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HWJ20192.1"
                     /translation="MIDFARFEWLSFDCYGTLIDWETGLLGYLRPLLENNGHALSDVQ
                     ILNLYSELEPRAQSGPYRSYREVLAQVVRDFARELHFSVGTAEANGLAESIRNWQPFA
                     DTVPALRRLQSRYKLAVLSNIDDDLFAYTAPKLEICFDAVVTAQQVHSYKPSFNNFEA
                     LLRRFQIPRERLLHVAESLYHDVVPAHALEIATVWVNRRQGKEAAATKLVEAHPDLEV
                     PNVGKLAELAVPVGQNYPLSS"
     gene            complement(746..1942)
                     /locus_tag="VNR65_15855"
     CDS             complement(746..1942)
                     /locus_tag="VNR65_15855"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWJ20193.1"
                     /translation="MKEFYICDCARLENQTITSLFVVAAKQVKSKKNGELYLSVTLAD
                     RSGQLQANMWDNVADTLDSFEQDDFVKVKGVVHKYNGRWQLTIHKLRKLEDPEIDYSD
                     YLPKTSKDIDGLWRTLSEFVESFENRWLKSLLQEFMADEAIVAAYKNAPAAKTLHHAF
                     VGGLLDHVVSLFTVCDLAARNYPQVNRDLLLSGAFLHDIGKLHELAYQRSISYTTEGQ
                     LLGHMIIELEMLHEKLAHLPGFPGELKILIEHMIISHHGHYEFGSPKLPMFPEALMLH
                     YLDDLDSKMESMRAQFEREADYDSPWTSYNPSLARPLLNTKKFLEKGLSASREAEARK
                     SAAVAGNGNTSPEPAAVQNVEAQPESAAAQPAEKEPEPSPLEALERKFASHKVTPVAG
                     TGKSSS"
     gene            complement(2317..3456)
                     /locus_tag="VNR65_15860"
     CDS             complement(2317..3456)
                     /locus_tag="VNR65_15860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWJ20194.1"
                     /translation="MKKFAVLLFAIFLGAPLVIAQSSQSAGQDETVVEEIIARINNNI
                     VTRADLRRSREQLLAESRQPDSSGLGPDTKGREQDLLRDLIDQQLLLQKGEELAISAD
                     TEVVKRLDELRKQMHAESMEDLEKAAQAQGVSWEEFKQNMKNSIISQRVISQEVGGHI
                     TVSQQEIQQFYDQHKSEMERPEQVRMSEILISTQKEPAVKTEKGQEVLPVAPSAEVVA
                     AARAKADKVYDLLKKGGKFEDLAKQYSDGPTAAAGGDLEYFKRGTLSKELEERVFAMD
                     PSGYTEPVRTNQGWVILKITEHQSPGIPPLKEVENRVQEQIYMTKMQPSLRDYLTKLR
                     EDAYIDIKAGYVDTGASPNETKPIYTTAATEGSSKNKKKKKWGLF"
     gene            complement(3709..4962)
                     /locus_tag="VNR65_15865"
     CDS             complement(3709..4962)
                     /locus_tag="VNR65_15865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HWJ20195.1"
                     /translation="MVSKNFCYFIIRTAAVFALVVMLLSSAWAATERPLYSFTGGNDG
                     GDPASRLTFDSSGNAYGTTVIGGVFGCGTVFQLTPMGGGQWQQSVLYSFGCFDDGKNP
                     YGGVTLDAAGNLYGTTVAGGTGGFCTGDGCGVVFKLTQSGGSWNESVLYSFTGDPDGF
                     GPGGGLVFDRAGNLYGTTPDGGAYAEGTIYQLSPNGGGWQEKVIHDFTGGNDGAVGSL
                     GLLLIDSTGSIYGVTELGGANGAGTVFKLFSSGGTWNLITLYAFRGQPDAAFPYGGLI
                     ADANGNLYGTTYFGGANGAGTVFKVAPGPNVIGGWKDTVLYSFQGGTDGGSPTSTLVF
                     NAAGNLYGTTSAGGDPGCDCGVAFELSPNGSGGWNESVLHTFGTAPDGAYLYYGMTLD
                     GMGNFFGTTVAGGAHGQGMVFELTP"
BASE COUNT         1026 a         1512 c         1490 g         1114 t
ORIGIN      
        1 ataggggtcc ttcgactcgc tgcgctcgct caggatgaca gcgggtaatt ctggccgacc
       61 ggcacagcca actccgccag tttgccgaca ttcggcactt ccagatcagg gtgagcttcg
      121 accagtttgg tggctgctgc ctctttgccc tgcctgcgat tgacccacac ggtggcaatc
      181 tccagcgcgt gcgcaggtac cacatcgtgg tacaagctct cggcaacatg cagcaggcgc
      241 tcgcgcggaa tttggaagcg gcgtaacaac gcctcgaaat tattgaacga cggtttgtaa
      301 ctgtgtactt gttgagcggt taccacggcg tcgaaacata tctccagctt cggcgcggtg
      361 taggcgaaca agtcgtcgtc aatattggag agcacggcca gcttgtagcg ggactgcaaa
      421 cggcgcagtg cggggaccgt atcggcaaag ggctgccagt tgcggatcga ttccgccagt
      481 ccgttggcct cggcggtgcc gacggagaag tgcagctcgc gagcgaagtc gcgaaccacc
      541 tgtgccagca cctcgcgata gctgcggtag ggaccgctct gggcgcgcgg ctccaattcc
      601 gaatagagat tgagaatctg gacgtcgctg agcgcgtggc cgttgttctc caataaggga
      661 cgcaggtagc cgagcagtcc ggtttcccag tcaatcaatg tgccgtagca gtcgaacgac
      721 agccactcaa agcgtgcgaa atctatcacg acgacgattt tcccgtgccg gccaccggcg
      781 ttaccttgtg cgaagcaaac ttgcgctcga gcgcttccag gggagacggc tccggctctt
      841 tttcggccgg ttgcgcggcg gcgctttcag gttgggcctc gacgttttgg acggctgcgg
      901 gttccggcga ggtatttcca ttgccagcaa ctgcggcgga ctttcgcgcc tccgcctctc
      961 gcgacgcgga cagtcccttc tccagaaatt tcttggtatt cagcagcgga cgcgccagcg
     1021 atggattgta gctggtccac gggctgtcgt aatcggcctc gcgctcgaac tgtgcgcgca
     1081 tgctctccat cttggagtcg agatcgtcaa gatagtgcag catgagcgcc tcgggaaaca
     1141 taggcagttt cggcgaacca aattcatagt gcccgtgatg gctgatgatc atgtgctcga
     1201 tcaggatctt caactcgccg ggaaagccgg gcagatgcgc gagcttctcg tgcagcattt
     1261 ccagttcgat gatcatgtgt cccagcaact ggccctcggt ggtgtaggaa atcgagcgct
     1321 gatacgcgag ttcgtgcagc tttccaatgt cgtgcaggaa ggcgccgctc agcagcaggt
     1381 cgcggttcac ctggggatag ttgcgggcgg caaggtcgca aacggtaaac agcgagacca
     1441 cgtggtcgag cagtccgccg acaaacgcgt ggtgcagcgt cttggccgcc ggtgcgttct
     1501 tgtacgcggc gacgatcgcc tcgtcggcca tgaattcctg gagcagcgat ttcagccagc
     1561 gattctcgaa gctctcgacg aattcgctca gcgtgcgcca tagcccatcg atgtccttcg
     1621 aggtcttggg aaggtagtcg gagtagtcga tctccggatc ttccagcttg cgcaacttgt
     1681 ggatggtgag ctgccagcgc ccgttgtact tgtggaccac acccttcact ttgacaaagt
     1741 cgtcctgctc gaacgagtcg agggtgtcgg cgacgttgtc ccacatgttg gcctgcagtt
     1801 ggccgctgcg gtcggccagg gtgacggaaa ggtagagttc accattcttt ttggacttta
     1861 cctgcttggc ggctaccacg aagagcgagg taatggtctg gttctccagc cgggcgcaat
     1921 cgcaaatgta gaattccttc atagcgggac ctgcgggcac atcgcgacaa gacaatgcgg
     1981 ccaggggttc atggtaccgc acttggctgt ggatggcgtg agagaaacgc aggaacgcca
     2041 accgcggtag aagaaagggc gtctccccgc gcgtccttca gactaagagc tacatgtaac
     2101 cacagagata ccgaggcaca gagaaggacg aaatcgacca cggacattca cggattctca
     2161 cggatttgat tttgaggttc ttgactggaa caaaatccgt gttcatccgt gaaaatccgt
     2221 ggtcaaaaca agccggttgc ggcggcaata agaatgccat gttctaattc aagaaggcgc
     2281 accgcagcgc gcctcccgtt aacttatcgc tgaaaactag aagagtcccc acttcttttt
     2341 cttcttattc ttcgacgagc cttcggtcgc ggcggtggtg tagattggct tggtttcgtt
     2401 ggggctggcg ccggtgtcga cgtagccggc cttgatgtca atgtaggcat cctcgcgcag
     2461 cttagtcagg taatcgcgta acgagggctg catcttggtc atgtagatct gttcttgaac
     2521 ccggttctcc acttctttca aaggaggaat gccggggctt tggtgttcgg tgatcttcaa
     2581 gatgacccat ccctgattgg tgcggacagg ctcggtgtag ccactcgggt ccatggcgaa
     2641 cacccgttcc tcgagctctt tggagagggt accgcgcttg aagtactcca agtcgccgcc
     2701 cgcggcggca gtcggaccgt cagagtactg tttggccagg tcctcgaact tgccaccctt
     2761 cttcaacagg tcgtaaacct tgtccgcctt ggcccgggcg gccgcgacca cttccgcgct
     2821 cggggccacc ggcagcactt cctgcccctt ttccgtcttc accgccggct ccttctgggt
     2881 ggaaatcagg atctcgctca tgcgaacctg ttcagggcgc tccatctcgc tcttgtgctg
     2941 gtcgtagaac tgctggatct cctgctgcga caccgtgatg tgcccgccca cttcctggct
     3001 aatcacgcgt tgcgagatga tgctgttctt catgttctgt ttgaattctt cccacgagac
     3061 gccctgcgcc tgggccgctt tctccaagtc ttccatggat tcggcgtgca tctgtttgcg
     3121 cagctcgtcg agccgcttga ccacctcggt gtccgcggag atcgccaact cctcgccttt
     3181 ttgcagcagc agttgctggt caatgaggtc gcggagcaag tcctgctcgc gccccttggt
     3241 gtcggggccc aaaccggagg agtccggctg gcgggactcg gcaagcaatt gttcgcggct
     3301 gcggcgcaga tcggcccggg tgacgatgtt gttgttgatg cgggcgatga tttcctcgac
     3361 gacggtttcg tcctggccgg ccgattggct ggattgggca attaccaggg gagcacccag
     3421 gaatatcgcg aacagaagca cagcaaattt cttcatcaaa ttccaatacc tgatcaggcg
     3481 cgcctacaca cgaaatctca cccgggaaaa tggccttact cgtacccgta ttctaccgcg
     3541 tctgaccggg ttgtgctggc cggcagcggc gtactcagaa atacgccgct ctctggagtg
     3601 ccgttagcag ttcaagccag acggtccaat aagccgcaag aagcgaatcg agccgaaggt
     3661 attgaagata aggagcacac gatcgcatcc gcgggcggag tttttaagtc acggcgtcaa
     3721 ttcaaatacc atcccttggc cgtgagcacc gccggctacg gtcgttccga agaagttgcc
     3781 cattccgtcg agagtcattc cgtaatacag gtaggctccg tctggagccg tgccaaaggt
     3841 gtggagtacg ctctcattcc atccgccact tccgttggga ctgagttcga aagccacgcc
     3901 acagtcgcat cccgggtcgc cacccgccga ggttgttccg tagagattcc cggctgcgtt
     3961 gaagaccagc gtactggtcg gagaacctcc gtccgtgccg ccttgaaagc tatacagcac
     4021 cgtgtctttc cagcctccga tgacgttagg gccgggagcc accttgaaca cggtgcccgc
     4081 tccgttggcg cctccaaagt aggtggtacc gtagagattg ccgttcgcgt cggcgatcaa
     4141 gccgccgtag ggaaaagccg cgtcgggctg gcctctgaag gcatacagcg tgatcagatt
     4201 ccatgtacct ccagaagaga acagcttgaa cacggtaccg gcaccgttgg cgccgcccag
     4261 ttcggttacg ccatagatgc tgccggttga atcgataagc agcagaccca gcgagcccac
     4321 agctccatcg ttgccgccgg tgaagtcatg aatcaccttc tcctgccacc ctccgccatt
     4381 gggtgacagc tggtaaatcg tgccctcggc atacgcacct ccgtccggcg ttgtcccata
     4441 gagattgccc gctcggtcga agactaggcc tccaccggga ccgaacccat cagggtcgcc
     4501 ggtgaagcta tacagcacgc tctcgttcca cgaaccgccg gactgggtca gtttgaaaac
     4561 cacgccgcaa ccatcgccag tgcaaaaccc gcctgtaccg ccggcgacgg ttgtgccgta
     4621 aagattgcct gcggcatcga gcgtcacgcc cccgtaggga tttttcccgt cgtcgaagca
     4681 gccgaagctg tacagcacgc tctgctgcca ctggcctcca cccatgggcg tcagttggaa
     4741 gaccgtccca caaccgaata caccgccaat cacggtagta ccgtaggcat tgcctgagct
     4801 atcgaaggtc aatcgcgaag ccggatcgcc tccgtcattc ccgcccgtaa agctataaag
     4861 cggcctctcg gtggccgccc aggcgctgct gagcagcatt acaaccagtg caaaaaccgc
     4921 agcagtccga attatgaagt agcagaaatt tttgcttacc attttccatc tcctcgattt
     4981 agaaatttct tagatccgaa gacgaagatt tacccagacg aggcggcgaa cagaacttta
     5041 gcactgaatc ggccgcaaag aacgctgaga ttgcagtttt gacattgttt cttcactcag
     5101 cttcgggatc gccggatacg gttggaggcg aatgcgcccc cc
//
LOCUS       DATMGX010000969         4589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_456365, whole genome shotgun sequence.
ACCESSION   DATMGX010000969 DATMGX010000000
VERSION     DATMGX010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456365"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VNR65_15870"
     CDS             <1..1184
                     /locus_tag="VNR65_15870"
                     /inference="COORDINATES: protein motif:HMM:NF033792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HWJ20196.1"
                     /translation="LALCLAVILLSAGLNTVLSIAYPRSQLLSSQLALLLLSYDVLQL
                     SALLFLTGGLENPFAFLLIVPVAVSASTQRLVITAMLAAFTVVLATILARYHWPLPWT
                     DTQPPVLPLNYMLGLWSALVSCIVFIAFYAWRIEQEARQMAQALSAAELVLAREQRLT
                     ALDGLAAAAAHQLGTPLSTIALVAKELEREVPKDSPARDDILLLQTQTARCRDILSQL
                     SKSGREGEEDIIFSKMRLRHLIEEVVAPLRTPGVEIDVLLADGSPQSKPTTEPVITRN
                     PGLMHSLVNIVENAVDFASERVTIDATYNQNKVLIRIGDDGPGFHPNVINLLGEPYVT
                     NRPRAEGDREESGMGLGFFIAKTLLERSGASIQIANRVLPETGALVEISWPRDRFEAS
                     S"
     gene            1287..1841
                     /locus_tag="VNR65_15875"
     CDS             1287..1841
                     /locus_tag="VNR65_15875"
                     /inference="COORDINATES: protein motif:HMM:NF033791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HWJ20197.1"
                     /translation="MATLAVSDPRPDNSLLIVDDDKPFLSRLAKAMEARGYSVRTAET
                     LEAGKQAIRDLPPAYAVVDMRLEDGNGLDVIRFLRETRPDCRALVLTGYGNIATAVNA
                     VKLGAVDYLSKPADAEDILAALTASGEAKPKPPENPMSADRVRWEHIQRVYELCNRNV
                     SETARRLHMHRRTLQRILAKRAPK"
     gene            complement(2165..2902)
                     /locus_tag="VNR65_15880"
     CDS             complement(2165..2902)
                     /locus_tag="VNR65_15880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ20198.1"
                     /translation="MKQRIVAVLAILGILGGGMALRASFAQTMHEMPAGGGDMSRHHD
                     HHMDGSSDGAFHRSFDDAEKWAKEFDKPERDAWQKPEEVVDALHLDRASSVADVGAGT
                     GYFSMKIAKRIPEGKLFAADVEPDMVRYLGERAQREHVANLAPVQANADAANLPEPVD
                     LILVVDTYHHIGNRIPYFAELKSALRPGGRLAIIDFKADSPIGPPVEHRILPEKVTEE
                     LAAAGYKLEESHDFLPRQYFLVFKAGS"
     gene            3480..4100
                     /locus_tag="VNR65_15885"
     CDS             3480..4100
                     /locus_tag="VNR65_15885"
                     /inference="COORDINATES: protein motif:HMM:NF018001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HWJ20199.1"
                     /translation="MSDKPLMPKATAVWLVENTSLTFEQIADFCGLHVLEVKGIADGD
                     VAHGIRGLDPITSGQLSREEIARAQKDSAYRLKLLRSKVFIPEIKAKRAPKYTPVSRR
                     HDRPNAILWLLRHHAELRDTQIMRLVGTTKSTIEAIRERTHWNSANLQPQDPVALGLC
                     SQIDLDSEVQKAAKRAADERKDEPVPAGVTIQPLEDQPYIDIEEWK"
     gene            4129..>4589
                     /gene="thyA"
                     /locus_tag="VNR65_15890"
     CDS             4129..>4589
                     /gene="thyA"
                     /locus_tag="VNR65_15890"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HWJ20200.1"
                     /translation="MQTYLDLLRMVRERGVKRGDRTGTGTLSLFGHQMRFDLGQGFPL
                     LTTKKLHVKSIIHELLWLLSGDTNTAYLKANGVSIWDEWADEHGDLGPVYGAQWRSWP
                     AGDGGGGSIDQIKELQHALKTDPNSRRHIVTAWNPADLPRMALAPCLCLFQ"
BASE COUNT          912 a         1423 c         1310 g          944 t
ORIGIN      
        1 cgcttgcgct ctgtctggcc gtcattcttc tgtcggcggg cctgaacacg gtgctcagca
       61 tcgcctatcc gcgctcgcaa ctcctctcga gccaattggc actcctgctt ctcagctatg
      121 acgtgctcca attatcggct ctccttttcc tgacgggcgg cctcgaaaat ccatttgcct
      181 tcctgctgat tgtcccggtc gcggtatcag cgtcgacgca gcggctcgtc atcactgcaa
      241 tgctggcggc gttcaccgtg gtcctggcca cgatccttgc acgctatcac tggcccctgc
      301 cctggacgga cacccagcct ccggttctgc cgctgaacta tatgcttggg ctttggtctg
      361 ctctcgtgtc gtgcatcgtg ttcattgcct tttatgcgtg gcgcatcgag caagaggccc
      421 ggcagatggc ccaggcgcta tccgcagccg agctcgttct cgcgagggag caacggctga
      481 ccgcgcttga cgggctggcc gccgccgccg cccatcaact cggcaccccc ctctcaacga
      541 ttgcgctcgt cgcgaaagag ctggagaggg aggttccgaa ggacagcccc gcgcgcgacg
      601 acatcctcct ccttcaaaca caaacggcgc gttgccggga tattctgagc cagctttcca
      661 aaagcgggcg cgagggcgaa gaagacatca tcttctcgaa aatgcggctt cgtcatctca
      721 tcgaggaggt ggttgcgccc cttcgcacgc cgggggtcga gatcgatgtt ctcctcgccg
      781 acggatcgcc gcagagcaag ccgactacgg aaccggtcat tacacgcaat ccgggtctga
      841 tgcacagcct cgtcaacatt gtggagaatg ccgttgactt tgcgagcgag cgcgtgacga
      901 tcgacgcaac ctacaatcag aacaaggtgt tgatccgtat cggagatgat ggcccgggct
      961 tccacccaaa cgtcatcaat cttctcggcg agccgtatgt gacaaatcgt ccgagggctg
     1021 aaggcgaccg cgaggaaagc gggatgggtc ttggcttctt tattgccaag acactgcttg
     1081 aacgctctgg cgcttccatt caaatcgcaa atcgggtatt gcctgagact ggagcacttg
     1141 ttgaaatttc ttggcctcgc gaccggttcg aggcgtcatc ttagattgaa aagcaatgga
     1201 gatagcagca taaattttgt gcgctcctgt tttgtgtcgt atactgcgac aacatgtagg
     1261 gtgtgacaaa aaggtaagtg cagcacatgg cgacgctcgc agtctcggac ccccggccag
     1321 ataattccct cctcatcgtg gacgacgaca agccgtttct ttcccggctt gcaaaggcca
     1381 tggaggctcg cggctacagc gtgcgcacgg cggagacgct tgaggcgggc aagcaggcga
     1441 tccgggattt gcccccggcc tatgcggttg tcgatatgcg tcttgaagac ggcaacggcc
     1501 tcgatgtcat ccgtttcctg cgcgagacgc ggccagactg tcgcgcgctc gtcctgaccg
     1561 gctacgggaa catcgcgacc gcagtcaacg cggtcaagct cggcgcggtc gattatcttt
     1621 ccaagccagc agatgccgaa gatattttgg ccgccctgac ggcctcaggc gaagcgaagc
     1681 caaagccgcc ggagaacccc atgtcggcgg atcgcgtccg ctgggagcac attcagcgcg
     1741 tctacgaact atgcaatcgc aacgtttccg agacggcccg ccggctgcat atgcatcgcc
     1801 gcacgctgca acgcatcctc gcgaagcgcg cgccgaagta aatcgcctgt cttggcatac
     1861 cagttccaag cagaagcctc gccttagccc taccgcgggt gatccattat ctgttaagcg
     1921 cagtttgggc ggtctgccgc taagcagagt tcgctgatct gaccaacgga ttatcatccc
     1981 ggaagccgtt cgcctcacgc tcgtcatccc ggaaaggcga agccttatcc gggatcgcaa
     2041 gtaattgaag cgcttatctt tttgcgatcc cggctcgcgc ttcgcttggc cgggatgacg
     2101 gcggcggcga tccgttggcg cgtccgcgaa atcctgctta gcccgtgccg tttgccgggc
     2161 gttcttagga acccgccttg aaaacgagga aatattgcct cggcaggaag tcgtggcttt
     2221 cttcgagctt gtagcctgct gcggccagct cttccgtgac tttctcgggc aaaatgcggt
     2281 gctcaacggg cggcccgatc ggcgaatccg ccttgaagtc gatgatcgca agtcgcccgc
     2341 caggacgcag cgccgacttc agctcggcga aataggggat cctgttcccg atgtgatgat
     2401 aggtgtccac cacaagaatg aggtcgacgg gctcggggag gtttgcggca tccgcattcg
     2461 cctggacagg cgcaaggttt gcaacatgct cgcgttgcgc ccgctcgccg aggtagcgca
     2521 ccatgtccgg ctcgacatcg gccgcgaaga gtttgccttc cggaatacgc ttcgcgatct
     2581 tcatgctgaa gtagccggtc ccggccccga catccgccac gctggaagca cggtcgagat
     2641 gcagagcgtc aacaacctct tccggcttct gccaggcgtc gcgttccggt ttgtcgaact
     2701 ccttggccca tttctccgcg tcgtcgaagc tgcgatggaa ggccccgtcg gatgagccat
     2761 ccatgtggtg gtcatgatgc cggctcatat cccctcctcc ggcaggcatt tcgtgcatgg
     2821 tttgcgcgaa agaggcgcgc aacgccatgc cgccccccag aatcccaaga atagccaaga
     2881 ccgccacaat ccgctgtttc actttttcgc cttttgatct ggaagttgaa ctcgccgaga
     2941 gccggccccg aaaaggcgac gcgaagcccc gcaagcggag ctccggccca aatcgttcat
     3001 ctcttagtcc gtgccaacca taacatcgga ggactttata atggcatagg ccgacatgcc
     3061 ctccttgagc ttcaggtctt ccaccgcctc attcgtgatc gacgaggtaa cggtcgcgcc
     3121 gttcacatcg atcaggatgt gggatgttgt cgcgccttga tgaccttggt gatcttgccc
     3181 ttgagcacat tgcgcgcact gattttcatc gaagcctccg atcgttaaag cctgcttggt
     3241 tcaggccgtc tcgcatgaag acccacgcgc ggaaagttcg caactcaact tagaggaaag
     3301 gcgcagcata gcggatctgc gtcaaaatct ggccgtccac cggcattgaa tcgaccgttt
     3361 gcacaaaacg aaaccaccta ttatattgac ccccaaccct tcgattgcca ttccccacac
     3421 aatcgacaaa agacgcccag gaagccgaga gggatcgctt ccaggaacgg aggagattta
     3481 tgagcgacaa acccctgatg ccaaaggcga ccgccgtctg gctcgtagaa aacacctcgc
     3541 tgacctttga gcaaattgcg gatttttgcg gcctgcacgt gctcgaagtg aagggcatcg
     3601 ccgatggcga cgtggcccac ggtattcgtg gtttggaccc gattacatcg ggacagctct
     3661 ctcgtgaaga aatcgcgcgc gcccagaagg actcggccta caggctgaag cttttgcgtt
     3721 cgaaagtttt catccccgag atcaaggcaa agcgggctcc caaatatacg cccgtttcac
     3781 gccgccacga ccgccccaac gcgatcctct ggcttctgcg gcatcacgcc gagttgcgcg
     3841 acacgcagat catgcggctc gtcggcacga cgaagtcgac gatcgaggcc atccgtgagc
     3901 gcacgcactg gaattcggcc aatctgcagc cgcaggaccc ggttgcgctt gggctatgct
     3961 cccagatcga cctcgattcc gaagtacaga aggcggccaa acgcgccgcg gatgagcgca
     4021 aagacgagcc ggttccagcc ggggtcacaa ttcagccgct cgaggaccag ccctacatcg
     4081 atatcgagga gtggaaatag ccccccagaa tcgcggtatc gttccgtcat gcagacctat
     4141 ctggacctcc ttcgcatggt gcgcgagcgc ggcgtcaagc gcggtgaccg cacaggcacg
     4201 ggtacgctca gcctgttcgg gcatcagatg cggtttgatc ttgggcaggg ctttccgctt
     4261 ttgaccacca agaagctgca tgtgaaatcc atcattcacg agcttctctg gcttctctcg
     4321 ggcgacacga atacggccta tctgaaggcc aatggcgttt ccatctggga tgagtgggcc
     4381 gacgagcatg gcgatctcgg cccggtctac ggcgcgcaat ggcgatcgtg gcctgccggc
     4441 gacggcggcg gcggcagcat cgaccagatc aaggagctac agcacgcgct caagacggac
     4501 ccgaattcgc gccgtcacat tgtaacggcc tggaaccccg cagacctgcc gcggatggcg
     4561 ctggcgcctt gcctttgtct tttccagtt
//
LOCUS       DATMGX010000970         2074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_134221, whole genome shotgun sequence.
ACCESSION   DATMGX010000970 DATMGX010000000
VERSION     DATMGX010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134221"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VNR65_15895"
     CDS             <1..715
                     /locus_tag="VNR65_15895"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWJ20201.1"
                     /translation="AFKRASAWRITAVLPYYCYARQDRKDKPRVPISAKLVADLLETA
                     GASRALTLDLHAPQIQGYFDVPVDHLYGSPVLIDYFKQMKLPNLTVVSPDAGGVERAR
                     AFAKKLDAPLAIVDKRRVDVDVSEVMHLIGDVKGRSTLIIDDIIDTAGTLVKTADALL
                     REGATQVYAACTHPVFSGPAVERIENSRITEVVATDSVPLSAAAKKLSKIKILSVADL
                     LARGIRSIHEETSISELFI"
     gene            768..1484
                     /locus_tag="VNR65_15900"
     CDS             768..1484
                     /locus_tag="VNR65_15900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWJ20202.1"
                     /translation="MAQIIVEGAPRETRGKNEARRMRVAGRVPATLYGGKGKAISLAV
                     NTKQVTAILRSETGHNTLFQVDLGGTHEPAIVKDWQVDPVSGKLLHVDLLRIAMDVRM
                     KVKVPIHTFGEPAGVKVDGGVYEVVTREVEIECLPADIPTEFKVDISGLRLNQSLRAS
                     ELPLDKAKIKLLTEPDRVLSHVVALRVEEEKPADVAAIAGATPAEPEVIKKGKKEVEG
                     EEGEEGEKGAKAPEKTEKKK"
     gene            1515..>2074
                     /gene="pth"
                     /locus_tag="VNR65_15905"
     CDS             1515..>2074
                     /gene="pth"
                     /locus_tag="VNR65_15905"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWJ20203.1"
                     /translation="MRLIVGLGNPGPEYQWTPHNLGFLAIDEIANRAAIRVERPEGKA
                     LVGRGKVAGEEVVLAKPQTYMNLSGISVRELLGKYELGPEALLALYDERDLPWGMIRI
                     GERGRPGTHNGAKSLTSALGTQDFARLRLGCGPDHPVSDLAEYVLRPMKKGQLEVAAE
                     MIADAADAVELLSKEGLAAAMNKYNRR"
BASE COUNT          442 a          568 c          659 g          405 t
ORIGIN      
        1 cgcgttcaag cgcgcctctg catggcgcat caccgcggtg ctgccgtact actgctacgc
       61 gcgccaggac cgcaaagata agccccgcgt tccgatatcc gcgaagctgg tagcggactt
      121 gctggaaact gctggtgcca gtcgcgcgct cactttggat ttgcacgccc cgcagattca
      181 gggctacttc gacgttccgg tcgaccatct gtacggctcg cccgttctga tcgactactt
      241 caagcagatg aagctgccga atcttaccgt ggtttctccc gacgctggcg gcgtggaacg
      301 cgcccgcgcg tttgcgaaaa agctggatgc tccgctggcc atcgtggata agcgccgcgt
      361 ggatgtggac gtcagcgaag tgatgcactt gatcggagac gtgaagggcc gcagcaccct
      421 gatcatcgac gacatcatcg acactgcggg cacgctggtg aaaaccgcgg atgccttgtt
      481 gcgcgaaggc gctacccagg tttatgccgc ttgcacccac ccggtttttt ccggcccggc
      541 tgtcgagcgt attgagaatt cgcgaattac cgaagtggtg gcgacggatt ccgtgccgct
      601 ttctgccgct gcaaaaaagt tgagcaaaat caagattttg agtgtcgcgg accttctcgc
      661 tcgcggcatt cgttctattc acgaagagac ttcgatcagc gagttgttta tttgagattc
      721 tccggcggaa tttaagccgg aagtgattcc agggaaggat agaagctatg gctcagatca
      781 ttgtggaagg cgcgccccgc gaaacacggg gcaaaaacga ggcaaggcgc atgcgcgttg
      841 ccggcagggt gcccgcgacg ctctatggcg gcaaaggcaa ggcaatttcg ctcgccgtga
      901 acaccaagca ggttaccgcg attctccgtt ccgaaaccgg gcacaacacc ctgttccagg
      961 tggatctcgg cggaacgcat gaacccgcca tcgttaaaga ttggcaggtc gatccggtca
     1021 gcgggaagct cctgcacgtc gatcttttgc gcatcgctat ggacgtccgc atgaaggtca
     1081 aggtgcccat acacaccttc ggcgaacccg ctggcgttaa ggtggacggc ggcgtctacg
     1141 aagtcgtcac ccgtgaagtc gaaatcgaat gcttgcccgc ggacatcccg acggaattca
     1201 aggtcgatat tagcggcctg cggctcaacc agagcttgcg cgccagcgaa cttcctctgg
     1261 acaaggccaa gattaagctt ttgaccgagc ctgaccgtgt gctctcgcac gtcgttgcgc
     1321 tgcgcgtgga agaagaaaag cctgcggacg tggcggccat cgcgggagcg acaccggccg
     1381 agcccgaagt catcaagaag ggtaagaaag aagtggaagg cgaggaaggc gaggaaggcg
     1441 agaagggtgc gaaggcaccc gaaaagacgg aaaagaagaa gtagttctct ttcgctcggc
     1501 tcgcggtgct cactatgcgg ctcattgttg gccttggcaa tcctggcccg gagtatcagt
     1561 ggacgccgca taacctcggt tttctggcca ttgatgaaat agcgaatcgc gctgcgatcc
     1621 gcgtcgagcg gcccgaaggc aaggcgttgg tgggacgagg caaagttgcc ggggaggaag
     1681 tggtcctggc gaaacctcag acctatatga acctcagcgg aatttccgtc agggaactgc
     1741 tgggcaagta tgagctgggg ccggaagcgc ttctcgctct ttacgacgag cgggatttgc
     1801 cttggggcat gattcgaatt ggtgagcgag gcaggccggg gacccacaat ggggccaagt
     1861 cgctcaccag tgctttgggc acgcaggact ttgccaggct tcgtctggga tgcggccccg
     1921 atcatccggt cagcgacttg gcggagtatg tgctccgccc gatgaaaaag gggcagttgg
     1981 aagttgctgc ggaaatgatt gcggatgcgg ccgacgcggt ggagttgctg tcgaaagaag
     2041 gtctcgccgc cgcgatgaac aagtacaacc ggcg
//
LOCUS       DATMGX010000971         5890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_360956, whole genome shotgun sequence.
ACCESSION   DATMGX010000971 DATMGX010000000
VERSION     DATMGX010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360956"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VNR65_15910"
     CDS             <1..904
                     /locus_tag="VNR65_15910"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWJ20204.1"
                     /translation="SLAIGTLDGVQRGHRAVLNAAKANARAGGLAVGAMMFEPYPRMY
                     FQPQKPFFRLTPLPRKLQLLSGCGLDFTAVIPFDKTLAELPAVEFVRHVLVEGFAIKH
                     ASVGYNFYFGKGRGGNPSVLAEQGRIHGFGVTVVEAQGDDGDMFSSTRIRELLAEGDV
                     GAAAEMLGSWWRISGVIVGGAGRGTIMGFPTANIRLEEGVTLKHGIYAVNAYLEGQKL
                     QGAAYLGTRPTFDAGLPVLEIFLFDFDGDLYGKTMEIEFIGFIRDDLKFRSMEALAVQ
                     IAEDCAKAKDILNRVADTPPPWNA"
     gene            complement(961..1371)
                     /locus_tag="VNR65_15915"
     CDS             complement(961..1371)
                     /locus_tag="VNR65_15915"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HWJ20205.1"
                     /translation="MSIKAEPAETLQAPAVAVRQMKTKGRAVIALINIPKHSVILQSH
                     AVLVSKEDSMKLGDTGLWPYRFALGDQCAFVFGEMSFCNHSDTPNAAISWTQLTPASA
                     VASLIALTDISANSEIEIFYADAEEYHRRGVALS"
     gene            complement(1529..2533)
                     /locus_tag="VNR65_15920"
     CDS             complement(1529..2533)
                     /locus_tag="VNR65_15920"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HWJ20206.1"
                     /translation="MQTEADSQLNGQSETFAQFVNRVDTIVFPGAMGTELQRRGYRST
                     LPLWSAGANLEAADLVAQIHSDYFAAGADVAITNTFRTTPRAFRKLGREGEARAALEK
                     SMAIALRATSDTGRAAFVGGSFAPLEDCYSPASVPSESELRQEHRLHATWLAEAGADF
                     LLPETINSGIEAQIMAEAASVTGLPFIISFVVDACGRLLDGTPIAEAVEKTDLPGRVG
                     VSLNCRPVDIIDEAFLTLSSVYDGAIGLYPNGIGCPHDDLGWCFTETDDDVEKFVSSA
                     LRWKRQGVKIIGGCCGTTPDYIRELRKALLKAKGKPACHSYHSGQQRHGANRPLSLPR
                     "
     gene            3086..4648
                     /gene="nifB"
                     /locus_tag="VNR65_15925"
     CDS             3086..4648
                     /gene="nifB"
                     /locus_tag="VNR65_15925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HWJ20207.1"
                     /translation="MMFDSEQKSEAIEALSKSLGGGLLNILPSGGCNSKGGSGKSSCG
                     SAAGQNDLSPEVWEKVKNHPCYSEQAHHHYARMHVAVAPACNIQCNYCNRKYDCANES
                     RPGVVSERLTPEQAAKKVIAVASTIPQMTVLGIAGPGDPLANPAKTFKTFELVAEQAP
                     DIKLCLSTNGLALPQQIETILKYKVDHVTITINAVDPEIGAKIYPWIFFNHRRYTGVE
                     AASILLDHQLRGLEMLTERGILAKINSVMIPGINDQHLIEVNRAVKARGAFLHNIMPL
                     ISAPEHGTAFGLSGQRGPTAQELKALQDACEGDMNMMRHCRQCRADAVGLLGEDRSDE
                     FTTEKVMAMEVEYDLEARKAYQAGVEKEREATANAKNAELATLAKEMSDVSILVAVAT
                     KGGGRVNEHFGHAKEFQIYEISTSGVKFVGHRRVDHYCQDGFGDEDKLDTVIRAINDC
                     TAVLVARIGTCPKETLEKAGLDPVEKYAHEFIEQSAVAYFKEYLDKIRRGEITHAPRG
                     DAEIRQGAFTAA"
     gene            4679..4897
                     /locus_tag="VNR65_15930"
     CDS             4679..4897
                     /locus_tag="VNR65_15930"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWJ20208.1"
                     /translation="MAYKIIASSCTVCGACEAECPNVAIRTKGGTYIIEAKKCTECLG
                     HFDQPQCVAVCPVENTVVIDTSVPRAQA"
     gene            4911..5453
                     /locus_tag="VNR65_15935"
     CDS             4911..5453
                     /locus_tag="VNR65_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20209.1"
                     /translation="MSPATELGAEAVFPVEDAVLTAAVLGAGLPEAAEQHLRLAASSY
                     RDDEVSEAHLWQAKAAAPDHPAVLIGLYRFYFYKNRLAEALSVARACLEKAAKDNGLP
                     LDWREVKPTDAEFSDFGAILPRFYLFTLKGYAYLNMRLGHIEEGRAAVAKLLELDPSD
                     KVGAKVLLGVLERMGRDDDG"
     gene            5446..>5890
                     /locus_tag="VNR65_15940"
     CDS             5446..>5890
                     /locus_tag="VNR65_15940"
                     /inference="COORDINATES: protein motif:HMM:NF017312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe4S-binding leucine-rich repeat protein"
                     /protein_id="HWJ20210.1"
                     /translation="MADIDEAIDWLGGAIFCDGCAHQDLLAADRCQPKHACVHDRYAR
                     RIDRFFDWNRDLANQYLDHPYFEVRAIATKWADVFRLPALLSDPEETVRWNAARRLPR
                     RDLLDLRNDPHREVRIRVATLLDPPDLTAMMRDEDYYVRIVVARRI"
BASE COUNT         1250 a         1733 c         1729 g         1178 t
ORIGIN      
        1 gtcgctcgcg atcggcactc tcgatggcgt acagcgcggg catcgggcgg tgctcaacgc
       61 ggcaaaggcg aacgctcgcg cgggcggcct tgccgttggg gccatgatgt tcgagcctta
      121 ccctcggatg tacttccagc cgcaaaagcc gttcttccgg ctgacgccgc ttccgcgaaa
      181 gttgcagctg ctttcggggt gcgggctgga tttcactgcg gtcattcctt tcgacaagac
      241 gctggcggag ctgcccgccg tcgagttcgt acgccacgtc cttgtagagg gctttgcgat
      301 caaacacgcg agcgtggggt acaacttcta ctttgggaaa ggccgcggcg gcaatccgag
      361 cgttctcgcc gagcaaggcc gcattcatgg attcggggtg acggtggtgg aagcacaggg
      421 agatgacggc gacatgttct catccacacg catcagggaa ctcttggccg agggcgatgt
      481 tggcgccgcc gccgagatgc ttggctcgtg gtggcgcata tcgggcgtca tcgtgggagg
      541 cgcggggcgc ggtacgatta tgggatttcc gaccgccaat atccgcctcg aagaaggcgt
      601 caccctgaag cacggcatct acgcggtgaa cgcctatctc gaaggacaaa agctccaggg
      661 cgcggcctat ctcgggacgc ggcccacctt cgacgccggc ttgcccgtcc ttgaaatctt
      721 cctgttcgat ttcgacggcg atctttatgg gaagaccatg gagatcgagt tcatcggctt
      781 cattcgcgac gacttgaaat tccgttcgat ggaggcgctc gctgtccaga ttgccgagga
      841 ctgcgcgaag gcgaaggaca tcctcaatcg cgtggccgac acccctccgc cctggaacgc
      901 ttgagaaggg cttttgtggt cggctatcgc ggttgcatct gctggccgaa aaaacggtac
      961 ttaggagagg gcgacccctc tccgatggta ttcttcggcg tcggcataga aaatttctat
     1021 ctcagaatta gcactaatgt ccgtcaaagc gatgaggctg gcaaccgcag aggcgggtgt
     1081 cagttgggtc caggaaatcg cggcgtttgg agtatctgag tggttgcaga aggacatctc
     1141 gccgaatacg aaagcgcatt gatcgcccaa cgcaaaccgg tacggccaca accccgtgtc
     1201 acccaatttc atggaatctt cttttgacac caagacagcg tgcgattgaa ggataaccga
     1261 gtgcttgggg atgttaatga gggcaataac ggccctgccc ttggttttca tctgccgcac
     1321 ggcaacggca ggcgcctgaa gagtttcagc cggctcagct ttaatggaca tgaatatatt
     1381 ccccgctcat cccgcaacga gcttaataaa agttcaagca ctagattttg attttagtca
     1441 aaaatatccg ggtcgtcgcg caaaaccggg cgcagtgatc gccgggtttg cgcgcagtgc
     1501 gggcggcgct gcgaaaatgt ccgaggcttc accgcggcag gcttagaggc ctattggcgc
     1561 catgcctttg ctgaccggaa tggtaagaat ggcacgctgg cttccctttt gccttcagga
     1621 gtgcttttcg cagttcacga atatagtctg gggtagtgcc gcagcatccg ccgataattt
     1681 tcacgccctg gcgcttccag cgcaatgcgc ttgagacgaa cttttcgaca tcatcgtccg
     1741 tttcggtaaa gcaccatccc aaatcatcgt gaggacaccc gatcccattg ggataaaggc
     1801 cgattgctcc gtcgtaaacc gaagacaaag tgaggaaagc ctcgtcaatg atgtcaacag
     1861 gcctgcaatt caaagagacg cctacacggc cgggaaggtc cgtcttctca acggcctcgg
     1921 cgatgggggt tccgtcgagt agccgtccgc atgcatccac gacgaaacta atgataaacg
     1981 gaaggcctgt gacggaagcg gcctccgcca tgatttgtgc ttctatgcct gagttgatcg
     2041 tctccggcag aagaaagtcg gcgcccgctt cggcaagcca agttgcgtgt agccgatgtt
     2101 cttgccgaag ctcgctttcg cttggcaccg aagcgggaga atagcaatcc tcgagcggtg
     2161 caaacgatcc accaacgaat gcggcacgtc ctgtatcact cgtggcccgg agtgcgatcg
     2221 ccatcgattt ttcgagcgca gcacgggctt caccctccct gccaagtttc cggaaggcgc
     2281 gaggcgtcgt tctaaaggtg ttggtgatag caacatcggc cccagccgcg aaatagtcgc
     2341 tgtggatctg agcaaccaaa tcagccgctt caagattggc tccggccgac caaagcggca
     2401 gggtgctcct atatccgcgg cgctggagct ctgtccccat tgcgcccggg aaaacaattg
     2461 tgtccacccg gtttacaaat tgggcaaagg tttcagattg tccgttcaat tggctatcgg
     2521 cttcggtttg cacgcgtttg tgccttagtg gttattgcgg cttggcgttt caatcgttcc
     2581 actataccat catttaggaa tggttttcta tggggcctcc gccttacaag aacaattaga
     2641 aatttgtcct gataccgagg catgcaatga aagcttattg caagtagcgt catagcctcg
     2701 caaggttttc ctattttgac gccatgcgta gccagccctt tggacggcga cgcacgggaa
     2761 tggcgaaacc cgtaacggcg agcagcgagc aggttctgag cacaagtgac atcctgaaaa
     2821 gctgcaagtt cgatgttggc gaaggcggca agctctgctt aagcgctgtc tcctttcgta
     2881 caaagctcgc gccgctgggg atgcttgtcg caaaagcgac agcgttccca accgccagcc
     2941 ttctctcgaa aaatcagcaa cttagcggcg tggcacggtc gttgctttag ggtttgccga
     3001 ggttcgttgc atcgtgagcg gtgcagcatt caaactggga tggatgcgag gtccatccga
     3061 gaatggccac ccttcgcgag gaccgatgat gtttgattcc gaacagaagt cagaagccat
     3121 cgaggccctg agcaaatcgc tcggcggagg gctgctgaat attctgccaa gcggaggctg
     3181 caactcgaaa ggcggcagcg gaaagtccag ttgcggttcg gccgcgggcc aaaacgacct
     3241 ctcccccgaa gtctgggaga aggtcaaaaa ccacccctgc tacagcgaac aggcccacca
     3301 ccactacgct cggatgcacg tggccgtcgc acctgcctgc aacatccaat gcaattactg
     3361 taaccgcaaa tatgactgcg cgaacgaatc ccgtcccggg gttgtcagcg aacgcctgac
     3421 accggagcag gccgcaaaaa aggttatcgc cgtcgcctcc acgatcccgc agatgacggt
     3481 gctcggcatc gctggccccg gcgatcccct cgccaatcct gcgaagacct tcaagacctt
     3541 cgaacttgtg gcggagcagg ctcccgacat caagctctgc ctctcgacca acgggctcgc
     3601 gctacctcag cagatcgaga cgatcctcaa atataaggtc gaccacgtca ccatcaccat
     3661 caacgcggtc gatcccgaga tcggcgcaaa gatctatccc tggatcttct tcaaccacag
     3721 gcgttacacc ggcgtcgagg ccgcgagcat tctcctggac catcagcttc gcggcctcga
     3781 gatgctcacc gagcgcggca ttcttgccaa gatcaactcg gtgatgatcc cgggcatcaa
     3841 cgaccaacac ctcatcgagg tgaaccgcgc agtaaaagcg cgcggcgcct tccttcacaa
     3901 catcatgccg ctcatttccg cgcccgaaca tggcacggcg ttcggcctct caggccagcg
     3961 cggtccgacg gcgcaggagc tgaaggcgct gcaggatgcg tgcgaaggcg acatgaatat
     4021 gatgcggcac tgccgccagt gccgggccga cgcggttggg ttgcttggtg aggatcgcag
     4081 cgatgaattt accactgaaa aggtcatggc tatggaggtc gagtacgacc tcgaagcccg
     4141 caaggcttat caggccggtg tggaaaaaga gcgcgaggca accgccaacg ccaaaaatgc
     4201 agagcttgcg acgcttgcca aagagatgag cgatgtcagc atcctcgtcg cggtcgcgac
     4261 caaaggcggc gggcgggtga acgagcattt cggccacgcc aaggaatttc agatctacga
     4321 aatctccacc tccggcgtga agttcgtcgg ccaccgccgc gtcgaccatt actgccagga
     4381 cggcttcggg gatgaggaca agctcgatac agtgatccgc gccatcaacg attgcaccgc
     4441 tgttcttgtg gcgcgcatcg gcacctgccc gaaagaaacg ttagagaaag ccggactaga
     4501 cccggtagag aaatacgcgc acgagttcat cgagcaatcg gctgtcgcgt acttcaagga
     4561 atacctcgac aaaatcagac gtggcgagat cacgcacgcg cctcgcggcg acgcagagat
     4621 ccgtcaggga gctttcaccg cggcttagcc gctcacttcg aagacaggag aaaatccaat
     4681 ggcttacaaa attatcgcct cgtcctgcac agtgtgcggc gcctgcgaag cggagtgccc
     4741 caatgtggcg atccggacga agggcggaac ttacatcatc gaagcgaaga aatgcaccga
     4801 gtgcctcggc catttcgatc agccgcaatg cgtggccgtc tgtccggtag aaaacacggt
     4861 cgtgatcgac acgagcgttc cgcgcgccca agcttaggga acggcaggca atgagtccgg
     4921 caaccgagct tggcgcagag gccgtctttc cggtggagga cgcggtcctg actgccgccg
     4981 tgctcggcgc cggattgccc gaggctgcgg agcagcacct gcgcctcgcc gcgagttcct
     5041 atcgcgacga cgaggtgtcg gaggcgcatc tgtggcaggc caaggcggca gccccggatc
     5101 atcccgcggt gctgatcggg ctctatcgct tctacttcta caagaacagg ctcgccgagg
     5161 ctctcagcgt ggcgcgggct tgccttgaga aggctgcgaa ggacaatggc cttccgctcg
     5221 attggcgcga ggtcaagccc acggacgcgg agttctcaga cttcggagcc atccttccgc
     5281 gcttctacct cttcacgctt aagggctacg cctatctgaa tatgcggctc ggccatattg
     5341 aagagggacg cgccgccgtc gccaagctcc tggaactcga tccaagcgat aaggtcggcg
     5401 ccaaagttct gctcggtgtt ctcgaacgca tggggcgaga cgacgatggc tgatatcgat
     5461 gaagccatcg actggctggg cggcgccatt ttctgcgatg ggtgcgctca tcaggacctc
     5521 ctggccgcag accgctgcca gcctaagcac gcctgcgttc acgaccgcta tgcacggcgc
     5581 atcgaccgct ttttcgactg gaaccgcgat ctcgccaacc agtacctcga tcatccttat
     5641 ttcgaggtgc gcgccatcgc caccaagtgg gcggacgtgt tcaggctgcc agctctttta
     5701 tccgatcccg aagagaccgt gcgctggaat gcggcgcggc ggttgccgcg ccgcgatctt
     5761 ctggatttgc ggaacgatcc gcaccgtgag gttcgcattc gggttgccac gctgctcgat
     5821 cccccggacc tcaccgccat gatgcgcgac gaggactatt acgttcgcat cgtggttgct
     5881 cgccggatcc
//
LOCUS       DATMGX010000972         2786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_143909, whole genome shotgun sequence.
ACCESSION   DATMGX010000972 DATMGX010000000
VERSION     DATMGX010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143909"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1018)
                     /locus_tag="VNR65_15945"
     CDS             complement(68..1018)
                     /locus_tag="VNR65_15945"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HWJ20211.1"
                     /translation="MTTMKTTLARRSLRALAAGTMLSLAAIATAAAAEQPAAAAKPAA
                     APAPAQEPAASPAPAEHYQVPRQEWTFGGLFGYFDEQQLRRGYKIYKNVCSNCHNMRF
                     LAYRNLGEPGGPNLSKEEVAALAAEIQVQDGYNDQGEPVMRAGKPSDYFQWRFKNEKE
                     AGASFNGAAPPDLSVMAKARTVERDIAWYSFPFLMLNDLFTQYQEQGPDYIYGLLNGY
                     VDAPASFKLNAGSNYNRVFPGHQIAMPQPLTDGVVDYEDGTPNTLDQESKDVVSFLAW
                     AAEPHLVERKKLGLMVLAYLAILAGLLFVAKKTLWRNVEH"
     gene            complement(1029..2303)
                     /locus_tag="VNR65_15950"
     CDS             complement(1029..2303)
                     /locus_tag="VNR65_15950"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HWJ20212.1"
                     /translation="MSHASTYEPKTAFTRWLDSRLPIIRFAAHFMAMPTPRNLSYLWT
                     FGAILTFFLVVQIITGVIVAMHYTPNTTLAFDSIERLMRDVNYGWLMRYTHMVGASLF
                     FLAVYIHMFRGLYYGSYKAPREVLWMIGVAILIAMIATAFMGYSLVWGQMSFWAVTVI
                     TNLFSSFDSIIPGLGTTLVQWVWGGFSVDNPTLNRLYSLHYLLPFIIVGLVALHVWAL
                     HVPGSNNPVGIEVRNQEKETLPFHPYFTTKDMFGLVVFAMLFAYFVFYVPNYLGDAVN
                     YVPANPLVTPPHIVPEWYFLPFYAILRAIPSKLGGVLLMFGSLVVLFFVPWLDTSRVR
                     STTFRPIYRWFFLALVVDVLALGYLGAIPPEGSAILAAKVFTLYYFAHFLVVMPIVGL
                     IETPRPMPSNISETFTHKGEGSGHDVRAVPAE"
     gene            complement(2331..>2786)
                     /gene="petA"
                     /locus_tag="VNR65_15955"
     CDS             complement(2331..>2786)
                     /gene="petA"
                     /locus_tag="VNR65_15955"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HWJ20213.1"
                     /translation="DASALSQASTEVDLSPVGVGQAITVLWRGKPIFIRHRTPEEIKM
                     AQAVPVEDLKDPYADVMGAPSSLPASDANRTKPGKENWLIAIGICTHLGCIPKGQAPG
                     DDRGLYGGWFCPCHGSMYDTSGRIRQGPAPRNLDIPPYDFTAATKIKIG"
BASE COUNT          632 a          756 c          856 g          542 t
ORIGIN      
        1 agcattttag aatcgatcaa cttttccgcc ttcaaccgat tccgatcaaa ggtggcttat
       61 ctgggcgtca gtgctcgacg ttacgccaca aggttttctt tgcaacgaag agcagccctg
      121 ccaggatggc gagataagcg agaaccatga gaccgagctt cttgcgctcg accaggtgcg
      181 gttcagccgc ccaggcgagg aaggacacga cgtcctttga ttcttgatcc agggtattgg
      241 gggtgccatc ctcataatcg acgacgccat cagtcagagg ctgcggcatg gcgatttgat
      301 gcccggggaa aaccctgttg tagttggaac ccgcgttaag cttgaagctt gctggcgcgt
      361 cgacgtaacc gttgagaagc ccataaatgt aatccggacc ctgctcctgg tattgcgtga
      421 acaaatcatt cagcatgagg aaggggaatg agtaccaggc aatatcgcgc tcgacggtgc
      481 gggctttggc catgacagac aagtccggcg gcgcagcgcc gttgaacgag gcgccagcct
      541 ctttctcatt cttgaaccgc cactggaagt agtccgacgg cttgccggcg cgcataacgg
      601 gttcgccttg atcgttatac ccgtcttgaa cctgaatctc ggccgcaagc gccgcgactt
      661 cctccttgga gaggttcgga ccgcccggtt ctccgaggtt tcggtaagcc aagaagcgca
      721 tgttatggca gtttgaacat acgttcttat agatcttata gccgcgcctg agctgctgct
      781 cgtcgaaata accgaagagg ccgccaaagg tccattcctg gcgagggacc tgatagtgtt
      841 ccgctggcgc aggcgacgct gccggctctt gagccggagc gggagcagcc gccggttttg
      901 cggctgccgc gggttgctcg gccgccgctg ctgttgcgat ggcagccagc gagagcatgg
      961 ttcctgccgc gagagccctc aaggaccgcc gggcgagagt agtcttcatc gtggtcattg
     1021 tccctctcct actcggccgg cactgcgcga acgtcgtggc ctgaaccctc gcctttatgc
     1081 gtgaaggttt cagatatgtt ggaaggcatc ggccttggcg tctcgatcaa accgacaatg
     1141 ggcatcacga caaggaagtg cgcgaagtag tacagtgtga agaccttagc cgcgagaatc
     1201 gcgctgccct ccggagggat tgcgcccagg taacccaacg caagtacgtc gaccaccaat
     1261 gccaggaaga accagcggta gatgggacgg aaggtcgtag accggacgcg agacgtatcg
     1321 agccagggca cgaagaagag cacgaccagc gatccgaaca tcagaaggac gccacccagc
     1381 tttgacggga ttgcccgcag gatcgcgtag aagggcagga aataccattc cggcacaata
     1441 tgcggaggag tcacaagggg attggcaggc acatagttca ccgcgtcgcc aaggtagttc
     1501 ggtacgtaga acacgaagta ggcgaaaagc atggcgaaca cgaccaggcc aaacatgtcc
     1561 tttgtcgtga agtatggatg gaaaggaagg gtttcttttt cctggttgcg gacttcaatc
     1621 ccgacagggt tgttgctgcc cggcacgtgg agggcccaga cgtggagggc aacgagcccc
     1681 acaatgatga agggcagcag atagtggagg ctatagagac ggttgagcgt cggattgtcg
     1741 accgagaagc cgccccatac ccactgcacc agtgtggtgc ctaaaccagg aatgatcgag
     1801 tcgaacgagg agaaaagatt cgtgataacg gtcacggccc agaagctcat ctgaccccag
     1861 acgagcgaat agcccatgaa agccgtcgca atcatcgcga tgagaatggc gacgccgatc
     1921 atccaaagca cttctctcgg cgctttgtat gacccgtaat aaaggccccg gaacatgtgg
     1981 atataaaccg cgaggaagaa cagcgacgca ccaaccatat gcgtgtagcg catcagccag
     2041 ccgtaattca cgtcgcgcat cagcctctcg atcgagtcga atgcgagcgt ggtgtttggc
     2101 gtgtagtgca tcgcgacaat gacgccggtg atgatctgaa ccacgaggaa gaaggtcagg
     2161 atcgcgccga acgtccagag gtaactcagg tttcgcgggg ttggcatcgc catgaagtgc
     2221 gcagcgaagc ggatgattgg caaacgggaa tcgagccagc gcgtgaacgc cgtttttggt
     2281 tcgtaggtcg aagcatgaga catatagagc aaccttcaga atgcctgggc ttagccgatc
     2341 ttaatcttgg tggccgccgt gaaatcataa ggcggtatat cgaggttacg cggcgcaggc
     2401 ccctggcgga tgcgtccgga agtgtcatac attgaaccgt ggcatggaca gaaccagcca
     2461 ccgtacagac cccggtcgtc gccgggcgcc tgacccttcg gaatgcagcc gagatgggtg
     2521 cagatgccga ttgcgataag ccagttttcc ttgccgggct tggtccggtt cgcgtcgctg
     2581 gcgggcaagc tcgaaggagc gcccatgaca tccgcataag ggtctttcaa atcctctacc
     2641 ggcacggctt gcgccatttt gatctcttcc ggcgtccggt ggcggatgaa gatgggtttg
     2701 ccgcgccaga ggacggtgat ggcttgtccg acgccgaccg gcgacagatc cacttcggtc
     2761 gatgcttgcg acagcgccga agcgtc
//
LOCUS       DATMGX010000973         3343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_436210, whole genome shotgun sequence.
ACCESSION   DATMGX010000973 DATMGX010000000
VERSION     DATMGX010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436210"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            650..1270
                     /locus_tag="VNR65_15960"
     CDS             650..1270
                     /locus_tag="VNR65_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20214.1"
                     /translation="MTDTRTQIAATLFRPAPGGYVYREPYRWPFGEAPHYLVNADQKA
                     ALLAIIVPKRPVLWQVVLWSTLCLMVAIACVGLWLYTGHDNPTALDILAMFILTVAQV
                     ILALAILFWWKRRRLRPLLATLTPTDLRITQIEMRAAATNAMSGKQLVMAGVAGVFAA
                     TTMLINAALQLAWRQPIGLLWLAGSLIFAGLAWYYFRQLVTRAEKP"
     gene            1597..2493
                     /locus_tag="VNR65_15965"
     CDS             1597..2493
                     /locus_tag="VNR65_15965"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWJ20215.1"
                     /translation="MIRLLALIALSAFLITPLMGAAPPPIPPLSERVTFLSADGATML
                     VGYIFRPQGPHAARMPAVVMMHGRAGVYSSAADGDYSAMTLSRRHQEWGHLWAQQGYL
                     AILVDGFGPRGYPQGFLRFSYFSRPETLNEVTVRPLDAYGALAWLRKRNDVIPDRIAL
                     QGWSNGGSAALATMASVSAPGVPSLTPVTGFRGALVFYPACGLKGQFDGGLVPYAPVR
                     VFQGSADEEISPRHCGELIDRSRARGGDIQITTYPSATHDFDDPGKDRRDVPANAAAT
                     KDAMPRAIAFFACVLNAQAVPK"
     gene            2816..3082
                     /locus_tag="VNR65_15970"
     CDS             2816..3082
                     /locus_tag="VNR65_15970"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HWJ20216.1"
                     /translation="MRMLALAILAMGMVSAAAPAQAQTYDPDYPVCMHVVGIGLNLED
                     CRYFTIDQCRAPASGRAAQCNINPFYAGATASQGRYDRRHRRVY"
BASE COUNT          626 a          980 c         1019 g          718 t
ORIGIN      
        1 cgagatgaaa ttcgtagaac agttgacctt ggtcactctt cagacggccc atcatggatc
       61 gacctcatcg agaatcgcgt caacacaacg gaatcacgct tcgcggtctg tctcaaccga
      121 cttttgcaac aaaatcggca cttttcggac ctgccaaccg gcatcgaaga tgtccggttc
      181 tcgggatagt gcggaagcgg ctgaccccgg gaggggcaaa tcacctgggc gatttatcga
      241 cccctctgtc cagtaccttt cgaaaaaaat tcggatttac agaaatcgca aatcacctta
      301 tatcggtcgc cgtcccgctc ctccgagggg tgttgcgcaa cgtcacgaaa cgtggagcgg
      361 gatgcggtgg acgccggcgg ggcgcttgac gggcgttgcg gcctgcggac ggcgaaggcg
      421 tgtggtcctg aagtccagct ttatttcctt catcaaagaa cggatttaac cacaagaacc
      481 agggacttgc gcccgacggg cgggaaagga aaaagcccgc ccgaacatgg cgggccccaa
      541 taggacctag ttcgccggca aaggcatcgg ctcagccccg gcagaagaag tcaaagccgt
      601 ttcccaagca cgtccattaa ggaggtatgc tgcgaaagag aggcacgcca tgacggatac
      661 gcgaacccag atcgccgcaa cactttttcg accggcgccg ggtggttatg tgtaccgaga
      721 gccatatcga tggcctttcg gggaagcgcc gcactatctg gtcaacgcgg atcagaaagc
      781 cgcactcttg gccatcatcg tcccgaagcg gccggtcctc tggcaggtgg ttctgtggag
      841 tacgttgtgc ctaatggtcg ccatcgcctg tgtcggactg tggctttaca ctggtcacga
      901 caacccgacc gcgcttgata tcctcgccat gttcatcctc acagtcgcgc aggtcatctt
      961 agcgcttgcg atcctctttt ggtggaagcg gcgccggctc cggccgctgc tcgcaaccct
     1021 cactccgacc gacctgcgca ttacgcaaat cgagatgcgt gcggcggcga cgaatgcgat
     1081 gtccggaaag cagttggtga tggcgggcgt agcgggcgtc tttgccgcaa ctacgatgct
     1141 gatcaacgcg gcccttcaac tcgcgtggcg ccagccaata ggtttgctat ggcttgcggg
     1201 cagtctcatc ttcgcaggcc tcgcttggta ttatttcagg caactggtca ctcgagcgga
     1261 gaagccttga acccaagacc tcgcgttcgt gagtatacgc cctacgccgg tcaaggcgaa
     1321 acacggtggc gacgcctttg cccctaccac ggttcggatt gaattcgtca accggacgcg
     1381 caccacggcc gcgacggcag gctgctttgc ctcgatgtcc ggttctgggg gaagaacaga
     1441 cctgctgggc ggacggtcag acttctcagt ttgaccccga aacagacatt gggtctgata
     1501 gggccgtgcg tccgccaacg gtgccgcatg tttgtcatct gggcctgacc ggggttcaaa
     1561 aagtttgtta ccaatgaacg taaggggggt tactggatga tcagacttct tgctctgatc
     1621 gcgctgtctg catttctgat taccccgctg atgggcgcgg ctccgcctcc gattccccca
     1681 ctctctgagc gcgtcacctt tctcagcgcc gatggcgcaa cgatgctggt cggctacatc
     1741 ttcagaccgc aaggccccca cgcggcaaga atgccggcgg tcgtcatgat gcacgggcga
     1801 gccggcgtct attcgtcggc ggcggacggt gactacagcg cgatgacact ttcgaggcgt
     1861 caccaggagt gggggcatct ctgggcgcag cagggctatc tcgccattct cgtggatggc
     1921 ttcggtccgc gcggttatcc gcaaggtttt ctgcgtttca gctacttctc ccggcccgag
     1981 actcttaacg aagtcacggt tcgcccgctc gacgcctatg gtgcgctcgc ctggcttcgc
     2041 aagcgcaacg acgtaatccc ggatcgcatc gcgttgcagg gatggtcgaa cggcggcagc
     2101 gccgcgctcg ccacaatggc gtcggtcagc gcacccggcg ttccgtcgct gacgccggtc
     2161 acagggtttc ggggtgcgtt ggtgttttat ccggcgtgtg gattgaaggg gcagtttgac
     2221 ggcggtctcg ttccctatgc gccggtcagg gtcttccaag gctccgctga tgaggagatc
     2281 tcgccgcgcc attgcggtga actgatcgac aggagccgcg cgcgaggtgg cgatatccag
     2341 atcacgacct atcccagcgc aacccacgac tttgacgatc ctggcaagga ccgccgcgat
     2401 gtgccggcga acgcggcggc aaccaaggac gcgatgccgc gtgccatagc gttttttgcg
     2461 tgcgttctga acgcgcaagc cgtccccaaa taggtattcg ctgttgggga tgcctgcgtg
     2521 gcgctgccaa cgagctgacc aaataagcgt cgtgttcgct gcggtgcatg agtccgttct
     2581 tgtcttggca cgaaccggac caacccggtc ggtccggcga tgttcgttcc tcggggtaat
     2641 ccggaagtgg cgtttcgggg ccgtcaggac cattgttgac ccgactcgga ctttgtctga
     2701 atttcccgca ccgcgcttgc gcaggctcgc gcgcgagtgc gaattgattg agatcaatat
     2761 ggtttgtctg tttcggtacg ttgatgctgt gccgtcgatc cagagggagg ttctgatgcg
     2821 catgctggct ttggcgattt tggcgatggg gatggtttcg gcggccgcgc cggcccaagc
     2881 gcagacgtac gacccggatt atccggtttg catgcacgtc gtcgggatag gactcaatct
     2941 cgaggactgc cgctacttta cgatagacca atgcagggcg ccggcttccg gccgcgcggc
     3001 ccaatgcaac atcaacccgt tttacgcggg cgcaacagcg tcgcagggac gatacgaccg
     3061 gcggcatcgc cgcgtctatt gagctaatcc aggctggacg gcctttggcg tcacaagcga
     3121 ctgccgctat tggcactctt gcgacatgcc gactgcatca ggcgatgtcc gctttcaggg
     3181 gtgccccgga agacatttgc tcagattgag ttcttccgag tttgaccgaa tgtatggtcc
     3241 ggccgtgcgt tgcaagaaga tttcgtcgaa ttggcgatga gcggtctttc atcaatgtat
     3301 ccggcctttg attggagcat tgctccgggc catcatggat atc
//
LOCUS       DATMGX010000974         2569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_174023, whole genome shotgun sequence.
ACCESSION   DATMGX010000974 DATMGX010000000
VERSION     DATMGX010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174023"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1915
                     /gene="groL"
                     /locus_tag="VNR65_15975"
     CDS             278..1915
                     /gene="groL"
                     /locus_tag="VNR65_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWJ20217.1"
                     /translation="MAAKDVRFGGDARQRMLRGVDILADAVKVTLGPKGRNVVLNKSF
                     GAPRITKDGVTVAKEIELADKFENMGAQMVREVASKSSDIAGDGTTTATVLAQSILRE
                     GAKAVAAGMNPMDLKRGIDKAVTAVVEELKIHTRKISTPAETAQVGTISANGDTEIGK
                     MISEAMQKVGNEGVITVEEAKGIATELDVVEGMQFDRGYVSPYFITNAEKMIADLDQP
                     YILIHEKKLSSLQPMLPLLESIVQSTRPLLIIAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEDLGIKLENVKLADLGNAKKILIEKENTTIVE
                     GVGKKADIQGRCTQIRAQIEETTSDYDREKLQERLAKLAGGVAIIRVGGSTEVEVKER
                     KDRVEDGLHATRAAVEEGIVPGGGVALARASLILGKLKADNDDQRFGMEIVRKAVLRP
                     LRQIVENAGEDGAVISGKVLDRDEYNWGFDAQTGEFKDLVKAGIIDPTKVVRSALQNA
                     ASVAGLLVMTEAMVAERPEKKEASIPPGGGGMGDMDY"
     gene            2109..2393
                     /locus_tag="VNR65_15980"
     CDS             2109..2393
                     /locus_tag="VNR65_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20218.1"
                     /translation="MHPLLHHGEGVQEQHLVRTQDGFTCAINLDHAGATLAQRSHEAV
                     QVADGLRRPQIREDHLNRQVGRDASLASKNPCPHCRQKRAVKPGKLVAKL"
BASE COUNT          567 a          743 c          831 g          428 t
ORIGIN      
        1 ccctatcgcc ctgcgatcgc ccgccacacc cggcgcatga caggcgcgac gatccgctcg
       61 gcctcggcct tgactatcgc tgcggccttg gcctcaacgt cgtccagccg ggtggtcacc
      121 acgccggttc ctcgcggccg atggggtgga aggcccagtg atgtaaaaac gtacggtagg
      181 ggatgtaagc ccgaacccca tgaggaaaaa tggggggtga gtagttacgg aggattcggt
      241 gacggcatcc aacagagtgt agagaaaggg taggaacatg gcggccaaag acgttcggtt
      301 cggtggagat gcccgtcagc gcatgctgcg gggcgtggat attctggctg atgctgtgaa
      361 ggtgacactg gggcccaagg gccgcaatgt ggtgctgaac aagagcttcg gcgcgccgcg
      421 tatcaccaag gacggcgtga ctgtcgccaa ggagatcgag cttgcggaca agttcgagaa
      481 catgggcgcc cagatggtgc gcgaagtggc cagcaagagc agcgacatcg ccggcgatgg
      541 caccaccact gccacggtgc tggcgcagtc gatccttcgt gagggcgcta aggctgtcgc
      601 ggccggcatg aacccaatgg acctgaagcg tggtatcgac aaggctgtta ccgccgtcgt
      661 cgaggaactg aagatacaca cacgaaagat ctccaccccg gctgaaaccg cccaggttgg
      721 gaccatctcc gccaacggcg atacagagat cggcaagatg atctccgaag cgatgcagaa
      781 ggtcggcaac gagggcgtga tcacggttga ggaagccaag ggcatcgcca ccgaactcga
      841 cgtcgtcgaa ggcatgcaat tcgaccgcgg ttatgtctcc ccgtatttca tcaccaatgc
      901 ggagaagatg atcgccgacc tggatcagcc gtacatcctg atccacgaga agaagctgtc
      961 tagcctgcag ccgatgctcc cgctgctaga gagcattgtg cagtcaaccc gtcccttact
     1021 gatcattgcc gaggacgtcg aaggcgaagc gctcgcgacg ctggtggtga acaagctgcg
     1081 cggtggcctg aaggtcgcgg ccgtgaaggc gccgggcttc ggcgatcgcc gcaaggcgat
     1141 gctggaagac atcgcgatcc tgaccggtgg caccgtgatc agcgaagacc ttggcatcaa
     1201 gctggagaac gtgaagctcg cggacctcgg caacgccaag aagatcctga tcgagaagga
     1261 aaacaccacg atcgtcgaag gcgtcggcaa aaaggccgat atccagggcc gttgcaccca
     1321 gatccgcgcg cagatcgagg agactacgtc ggactacgac cgcgagaagc tgcaggagcg
     1381 gttagccaag ctcgcgggtg gtgttgccat catccgcgtc ggcggctcca ccgaagtcga
     1441 agtaaaggag cgcaaggacc gtgtggagga cggcctgcat gccacgcgtg ctgcggttga
     1501 agagggcatc gtgccagggg gcggcgtcgc cctggcacgt gcgtctctca ttttgggcaa
     1561 gctcaaggcc gacaacgacg accagcgctt cggcatggag atcgtccgca aggctgtgct
     1621 gaggcccctg cgccagatcg tggaaaatgc gggcgaggat ggtgcggtga tctcgggcaa
     1681 ggtgctcgat cgcgatgagt acaactgggg ctttgatgca caaaccggcg agttcaagga
     1741 cctggtaaag gcgggcatta tcgacccgac gaaagtcgtg cgctcggcgc tgcagaacgc
     1801 cgcctccgtg gccggtctgc ttgtcatgac cgaagccatg gttgctgaga ggccagagaa
     1861 gaaggaggcc tcgatcccgc caggtggtgg cggcatggga gatatggact actaggacta
     1921 ggggcagacc caacattgac actggtggcg aattcaatgt tcgaagaacg ttctggcccg
     1981 aatttcgcct gatcaaccca ggtggggccg ctcaagcttg ccttggtaac gctcccacag
     2041 gtcaaaggct ggcaacgtgg cgaattcgcc accagtatca atgtccacag cagcgcggtg
     2101 gccgcatcgt gcatcccctc ctgcatcatg gcgaaggcgt ccaagagcaa cacctcgtcc
     2161 gaactcagga cgggttcaca tgtgcaataa atctcgatca tgcgggtgca accctggctc
     2221 aacgctcgca tgaagcggtc caggtggccg atggcctccg gcgtccgcag attcgcgaag
     2281 atcatctgaa tcggcaggtc ggccgggacg cctcgcttgc atccaagaac ccatgcccgc
     2341 attgccgcca gaagcgtgcc gtcaagccag gaaagctcgt cgcgaagctc taacaccgga
     2401 aaatcacgca cgggagcact cctttctgca attcggcgta agccgcttaa ttgcgaacga
     2461 ctctcagtcg cattacagag catcccccgc ggtgtgacgt caaggcgtca gaagtgagcg
     2521 gcccgggcat ctcgcccgcc gcatgctctg gccacgtgac ggaggcgag
//
LOCUS       DATMGX010000975         2181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_224149, whole genome shotgun sequence.
ACCESSION   DATMGX010000975 DATMGX010000000
VERSION     DATMGX010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224149"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2181
                     /locus_tag="VNR65_15985"
     CDS             <1..>2181
                     /locus_tag="VNR65_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20219.1"
                     /translation="KAQGASPDLAALAEGLTEDIVTGMSRFSYLRVIARGSTLSYANK
                     AIDLRVAGKELGARYVMEGTLRQAGAKVRLAVQLVDTVNGAHLWAENYERAFSPESIF
                     ELQDDLVPRIVSTVADNNGALPRSMSEAVHSKAPEELSPYEAVLRSFAYFERYTPEEL
                     SASRSGLEAAVRKAPAYADAWAMLACLCAQDYLHGYELQKNALESAASAARKAVELSP
                     ANHLAYFSLAQVLAHQKDFDSFQDAAERVLALNPMDGNAVAFLGEFLVYGGNAERGMQ
                     LVERAKQLNPNHPGFYWFADFYHAFSQKDYRGALAFAQKARLRGNPLASMMIASAAGQ
                     LGDGETAAKAAGDLLKFRPELPALMPRQVAKVWNPEFGERFLDGLRKAGLDIPAAQTP
                     SAPASTTSGATRIADSKPPSIAVLPFANMGADKGQEYFSDGLAEEIINLLAKIPGLKV
                     IARTSAFAFRGKETDIRGIAGALGVTHVLEGSVRRTESRLRVTGQLIDAADGAQLWSE
                     RYDREMSDIFTLQDDIATAIAGALRVKLSPETIPKRYEPKLAAYEAYLKAKYLQAKAT
                     PESLELAKQCHERAIKLDPAFALARVEVGFYWVAMTLFSRYPADHASRAAREEAERAL
                     QIDPALPDAHALSGYVAAVFEQDWTRAEQYFAFPGARQVGTSITQPVYAWFQYLRGNV
                     AEAIALAQRAVEEDPLDVWPRMNLHAYLQAADREREALEQLRKVL"
BASE COUNT          461 a          683 c          642 g          395 t
ORIGIN      
        1 caaggcgcaa ggcgcaagcc cggacctcgc cgcgttggcc gagggactta ccgaagacat
       61 cgtcaccggc atgtcgcgct tttcttattt gcgcgtgatc gcacgcggtt ccacgctcag
      121 ctacgccaac aaggcaatcg acctgcgcgt agcaggcaaa gaactcggtg cgcgctacgt
      181 catggaaggc accctgcgcc aggctggcgc aaaggtacgt ctcgccgtcc aactcgttga
      241 cacggtgaac ggcgcgcacc tctgggctga gaactatgag cgcgccttca gccccgaatc
      301 catcttcgaa ctccaggacg acctggtccc acgcatcgtt tccaccgttg ccgataacaa
      361 cggcgcattg ccgcgcagca tgagtgaggc ggtgcatagc aaagccccgg aagaattgag
      421 cccttacgaa gccgtgctgc gcagcttcgc ctactttgag aggtacaccc cggaagagtt
      481 gtcggcttcc cggtctgggt tggaagctgc cgtgcgcaaa gctccggcat acgcagatgc
      541 ctgggcaatg ctcgcatgcc tctgcgccca ggactatttg catggatatg agctacaaaa
      601 aaacgccctg gaaagcgcgg cctcagccgc acgcaaggcc gttgaactga gcccagcgaa
      661 tcatctggcg tatttcagcc tggcccaggt cctcgcacac cagaaagact tcgattcgtt
      721 tcaggatgct gccgagcgtg ttctggcgct taacccgatg gacggcaatg cggtcgcctt
      781 tctaggcgag ttcctcgtat atggcggcaa tgcggagcgc ggcatgcaac tggtcgagcg
      841 cgccaagcaa ctgaacccga atcatcccgg cttttactgg ttcgcggatt tttaccacgc
      901 gttttctcaa aaggactacc gcggcgcact cgctttcgca cagaaagcca ggctgagggg
      961 caacccgctg gcatcaatga tgattgcatc cgcggcaggt cagctcgggg acggagaaac
     1021 ggcggcgaaa gcggcaggcg acttgctgaa gtttcgcccc gaactccccg ccttgatgcc
     1081 caggcaagta gcaaaagtct ggaaccctga attcggcgaa aggttcctcg acggccttcg
     1141 caaagccggc ctcgacattc ccgccgcgca aacgccatcc gcccccgcca gcaccaccag
     1201 cggagccacc cgcatcgccg attccaaacc tccctccatc gccgtgctgc cattcgccaa
     1261 catgggcgcg gacaagggcc aggagtattt ctccgacggc ctggcggaag agatcatcaa
     1321 cctgctggca aaaatccccg gcctgaaagt catcgcccgc acctcggcct tcgcattccg
     1381 cggcaaagag accgacattc gcggaattgc gggtgcgctg ggcgtcacgc atgtgctcga
     1441 agggagcgta cgccgcacag aatcccgcct ccgcgttacc ggccagttga ttgacgcagc
     1501 cgacggcgcc cagctctggt ccgagcggta cgaccgcgag atgagcgaca tcttcaccct
     1561 tcaggatgac atcgctacgg caatcgccgg agcattgcgg gtgaagcttt cgccagagac
     1621 cattccaaaa cgctatgaac cgaagctcgc ggcctatgag gcttatctga aagccaagta
     1681 tctgcaagcc aaggctacgc cggagtcgct cgagttagcg aaacaatgcc acgagcgggc
     1741 gattaagctc gatccggcat ttgccctcgc tcgggttgag gtgggattct actgggtcgc
     1801 aatgacactg ttcagcaggt acccggcgga ccacgcttcg cgggcagcgc gtgaagaggc
     1861 cgagcgagcg cttcaaattg atccggctct cccggacgcc catgctctgt cgggatacgt
     1921 ggccgcggtc ttcgagcagg attggaccag ggctgagcag tatttcgcct ttccgggggc
     1981 aaggcaagtc ggcacatcga taacccagcc cgtttatgca tggtttcagt acttgcgcgg
     2041 caatgttgcg gaggcaatcg cgctcgcaca gcgtgcagtc gaagaagacc cgctcgatgt
     2101 atggccacgg atgaatctgc acgcctatct gcaggctgcg gaccgcgagc gggaagcgct
     2161 tgagcaattg aggaaagtgc t
//
LOCUS       DATMGX010000976         6880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_86478, whole genome shotgun sequence.
ACCESSION   DATMGX010000976 DATMGX010000000
VERSION     DATMGX010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86478"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..1580
                     /locus_tag="VNR65_15990"
     CDS             351..1580
                     /locus_tag="VNR65_15990"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HWJ20220.1"
                     /translation="MSSVTFTAEGESTGLLVPLRLPLRRVLAIDAFRGLTILAMIFVN
                     TLENVEGMPFWLYHTPENSDGLTFPDLVFPAFLFIVGMSIPFATAQRLAAGDTVWQLM
                     RHVLMRTLGLLVLGVFMVNAEEGYNEQSMPLSIGVWSLLFYASALMVWGIYSFQNKVL
                     ATALRVAGVMGLVVLAFMFRGGEDGSVHLSPKWWGILGLIGWSYLFASTIVLLGRSRV
                     LPLVIGLLIGLIYSCLGQLDGMQSSPLLQLLTAQSENAAHTSIVLCGVITTLLFFNAS
                     GDGTPQRFGSAALLALALLAFGALLQPYYGLSKNGATPSWALYSAALCVLLFAFLYWL
                     IDLKRARGWTAFLRPAAANPLMIYMLPSILCALMKTLHVALPEPLTEGMPGLLWAVAY
                     AFVMLAVAHRMRLKLQL"
     gene            complement(1587..1820)
                     /locus_tag="VNR65_15995"
     CDS             complement(1587..1820)
                     /locus_tag="VNR65_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20221.1"
                     /translation="MQNVVAEKSFARAKFVADDKRIETSFPAPVAPALSQLRSKAISG
                     DARYRRANGGSGRGFAAAVCPERRYRRGAGKGG"
     gene            complement(2619..2703)
                     /locus_tag="VNR65_16000"
     tRNA            complement(2619..2703)
                     /locus_tag="VNR65_16000"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2667..2669),aa:Tyr,seq:gta)
     gene            3131..4501
                     /locus_tag="VNR65_16005"
     CDS             3131..4501
                     /locus_tag="VNR65_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWJ20222.1"
                     /translation="MRKRGLALGAFLVVLFTATGFVTLLPSQERNAVAAGAEVYKSFE
                     LFGDVLEQVRANYVVKPDDSKLIENAIKGVLTGLDPHSSYLTPKEVEDEQAETRGEFG
                     GLGIDVTMEDGFVKVIAPIDNMPAERAGVYAGDAITAIDGEGLRGLSMDAAVDRMRGP
                     VGSPIVLTIARKGIPKPITVKVVRDVIRVNPVQYSVEGDVGWIKVKSFENEHTTDYLE
                     QAVADIKKSLGGRVAGYVLDLRNNPGGLLDQAIALSDAFLDHGLIVVTKGRNPSDIDR
                     AEAQAGDVTDGKPLVVLLNGGSASASEVVAGALQDHKRAQIVGTRSFGKGSVQTMIPL
                     PNKGAIRITTARYYTPSGRSIQATGIEPDYVVEPELPPELKLQLALLPFESEAQLRGH
                     LKNSGTEESGGSVSYVPREKEKDVQLKAAIAVLHGEFPQTGKKAIDQARAASDTTHPA
                     LGTSGG"
     gene            complement(4850..5017)
                     /locus_tag="VNR65_16010"
     CDS             complement(4850..5017)
                     /locus_tag="VNR65_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20223.1"
                     /translation="MTRQQQRIEELERENERLKKDLLSANEQIAQLKILHESQAMQIR
                     QLKERKILNRC"
     gene            5347..6045
                     /locus_tag="VNR65_16015"
     CDS             5347..6045
                     /locus_tag="VNR65_16015"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HWJ20224.1"
                     /translation="MVREHDVWLRNPYITCMNIIVFGPTGGTGRAVIAKMLADGHTVT
                     AFARDPSKLSPAPGLTIVKGDAMNPADVKHAIPGHDTVVISLGNPQLAIAISLGARRT
                     TPADICEIGTRNIVAALGPTSPVRVLAVTSFGVGDTRDKMQIAFKIFSQLLLREAMAD
                     KERQEAVLKASDLDYVLVQPVGLTDTPASGDWVASAKGETRKMRMSRKDVAAFIAEDI
                     ANPLYHRQTVALSG"
     gene            complement(6230..>6880)
                     /locus_tag="VNR65_16020"
     CDS             complement(6230..>6880)
                     /locus_tag="VNR65_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20225.1"
                     /translation="DRVRAASETAESFRARLARGTGSGGKSSKELVARLALQLHLIGE
                     GIKDVYEAAPIEAVLSVEPALEGGGDRHGDPEEWCEQLLAMYRAWAASRNMQLSEVPP
                     KAKEPPLLVIGGFGAYRTLSHEAGLHVLEMPGEEKSVDRVTARIRLAAAPLGEIPPNR
                     LPAALAIALSHSAPSNTLVRRYRNGASPLVRDMVAGWRTGRLDAVLQGDFDLIAAA"
BASE COUNT         1392 a         2028 c         1976 g         1484 t
ORIGIN      
        1 gcagaaccgc tggcaacgga accggaagat cggccgtgag attgtaccag aatttcggaa
       61 tctgcgtttc gtcgagaacg aatttgacct ggctcatcac gttaacctcc ccttagaaaa
      121 cgtcggcgga accatacacg aggaaagcca agctgcaatt gcgtcatttt ggataactct
      181 ggctccatcc cattcgagcg ttggagaaat ttccgctcat aaagcttccc attccggcaa
      241 cgcgcgcatc ccgccatcga aacgcccgga tcgttatcac catcgccccg ttccttcact
      301 gaaggcgctg cgcaagcagg tccggcattt gttttccgtt tggagatttc atgagttcag
      361 tgacgtttac ggccgaaggc gaaagtacgg gacttctggt accgcttcgg ctgccgttgc
      421 gacgcgtact cgcgatcgat gcatttcggg gattgacgat cctcgccatg attttcgtga
      481 acactctgga aaatgtcgag ggcatgccat tctggcttta tcatacgccc gaaaattcgg
      541 acggcctgac gttcccggac cttgtcttcc ccgcgttcct gttcatcgtc ggaatgtcga
      601 tccccttcgc taccgcccag cggcttgccg ccggagatac ggtctggcaa ctcatgcgcc
      661 atgtgttgat gcgcacgctc gggcttctgg tcctgggcgt cttcatggtc aatgccgagg
      721 aaggctataa cgagcagtcc atgcccctat cgatcggcgt gtggtcgctc ctgttttatg
      781 catcggcact catggtctgg ggtatctaca gctttcagaa caaggtgctt gccaccgctt
      841 tgcgcgtggc cggggtcatg ggacttgttg tgcttgcctt catgtttcga ggaggcgagg
      901 acggcagcgt gcatctttcg ccgaagtggt ggggcatcct gggccttatc gggtggtcct
      961 acctgtttgc cagcacgata gtcctccttg gacgcagccg cgtgcttccc ctggtcatcg
     1021 gcctgctcat aggcctgatt tactcctgcc tcggccagtt ggatgggatg cagtcctctc
     1081 ctttgctgca actgctgacc gctcagtcgg aaaatgcggc tcacacctcc attgtgctct
     1141 gcggcgttat cacaacgctg cttttcttca acgcaagcgg agacggaacg ccgcagcgtt
     1201 ttggatcggc ggcgcttttg gcgctggcgc tgcttgcctt tggagcgctc ctgcaaccgt
     1261 attacggact atcgaagaac ggggcgacgc cgagctgggc gctttacagc gccgcgctat
     1321 gcgttctgct gtttgctttt ctctactggc tgattgacct gaaaagggcg cgaggctgga
     1381 ccgccttcct tcgcccggcg gctgccaacc cgctgatgat ttatatgctc cccagcattc
     1441 tctgtgcgct gatgaagacc ctgcacgtgg ctttgcccga gccgctgacc gagggcatgc
     1501 ccggtctgct ttgggccgtg gcctatgcct tcgttatgct cgcggtggcg catcgaatgc
     1561 gactgaagct gcagctgtag catccttcac ccacctttcc ctgctccacg gcgataacga
     1621 cgttcgggac aaacggcggc agcaaagccg cgaccgctgc cgccgtttgc acgacgatac
     1681 cttgcatcgc cactgatcgc tttactacgc aattgcgaga gcgccggcgc gacgggtgct
     1741 ggaaacgagg tttcgatcct cttatcatcc gccacaaact tcgccctggc gaaggacttt
     1801 tctgcaacaa cattctgcat aaccgctcaa gattcgccga tagggcagat ggcaaagcgc
     1861 ggctccttta ccgttgacgc ttccacgacc ggggcagcct ttgagaaagc ctgcgagaaa
     1921 aacatgcttc taatccgcga catgatcatc gcgctctccg aggttacctc gcgagctgga
     1981 agagcttgtg aaagacgccg tgcgacaggg cgccagtccc agtatccgga cacgccgcgg
     2041 aactggcagg accggctcga aacggcgttc ctgtcgactg tcggcagcgg cagataggcc
     2101 ttagcgatgc atggtgaaac caaggagcgg ctcgacgacg tgtgcttcac agcacaatcc
     2161 ttgtgcaaat cttgccgtga cagcggcccc aggtgccggc aggcagcaca ctggaagtgc
     2221 tcgcaaccga tccgggctcg gtcgccgata tggccttctt ctgccgcatg acgggaaatg
     2281 agcttcccca atcaggcaca tggcctgcca cccgacgccc cgcaggggag agatccccta
     2341 gcagttgggc gcaaccgcta ggggtggagg ggcacttgcg tgcaggttgc tgaattaaat
     2401 taaaccagat tgagcacaat ccccgaacga ggggacttta tcgagcgcag tctagctccg
     2461 cgctgatacg ttgatatcta cttaatcact gccactggtt ctctagcgaa ccgagctcga
     2521 tgacgaacgc gctgcattat ctgtccacag cgcgcgaaag gcagcaaggc gaagctatcc
     2581 cggatgtgtc ttgtttgatt gagagatttg ataggaactg gtggaggggg ttggattcga
     2641 accaacgtag gcttagccaa cggatttaca gtccgtcccc tttagccgct cgggcacccc
     2701 tccccaagaa aacaccagca aaatcatgcc actaactctt gagctagatt ccgcgcgggt
     2761 ctaggccttg gtttgccgct tggcctgcaa tttgtcaatc cctttttgaa taagaacgtt
     2821 cttgttttgt tttttcactt accatctgac cgccgaatga ctcctccgag ccgttcccag
     2881 cctgcgaacc tctcgattac cggagagatg tgtacagtct gcaacgtgcg ctaccaaagc
     2941 ttcgcagcac gaaatgccgc acctctgatc gtggaacaac cgcaagatca accaatcttg
     3001 gcccaaatat tgccttctaa gcgagacagt gaggagaatt cgttaagaga aggtgaacgc
     3061 cttcctttta gcgaagcgcg tggccgatag cggttcacgc ctctcaggca tgctagaagg
     3121 ttaaaaagta atgcgcaaac ggggactggc gcttggtgcg ttcctagtcg tccttttcac
     3181 tgctaccggc tttgtcactc tgttgccatc gcaggagcgc aacgccgtag cggctggggc
     3241 cgaagtttat aagagtttcg aacttttcgg agacgtgctc gagcaggtgc gcgccaacta
     3301 cgtcgttaag cccgatgatt caaaactgat cgaaaacgcg atcaagggcg tcctgaccgg
     3361 gcttgatccc cattcgagct accttacgcc gaaagaggtt gaagacgagc aggcagaaac
     3421 gcgcggcgag ttcggcgggc ttggcatcga cgtcaccatg gaggacggct ttgtcaaggt
     3481 gatcgcgccc atcgacaata tgcccgcgga acgggcaggc gtctatgccg gcgacgccat
     3541 tacggcaatc gatggcgagg gtctgagagg gctcagcatg gacgcggccg tggacaggat
     3601 gcgggggccg gttggctcgc ccatcgtcct gaccatcgcg agaaagggca taccaaagcc
     3661 gatcaccgtc aaggtcgtcc gcgacgtgat ccgggtcaac cctgtccaat atagcgtcga
     3721 aggcgatgtc ggctggatca aggtcaagtc attcgagaac gagcatacga ccgactatct
     3781 ggagcaggcc gtcgccgaca taaaaaagag cctcggcggc agggtcgccg gctacgtgct
     3841 cgatctcagg aacaatccag gcgggcttct cgatcaggct atcgctctct ccgacgcttt
     3901 tctcgaccat ggccttatcg tcgtcacgaa aggccgcaac ccatccgata ttgatcgcgc
     3961 agaagcgcaa gcgggcgacg tcacggatgg aaaaccgctg gttgtcctgc tcaatggcgg
     4021 gtcggcttcc gcctccgaag tggtggcggg ggcgctgcaa gatcataagc gggcgcagat
     4081 cgttgggacg cggtccttcg ggaaaggctc ggttcagact atgatcccgc ttccgaacaa
     4141 gggcgcgatc cgtatcacaa ccgccagata ttacacgccc tccggccggt cgatccaggc
     4201 aacggggatc gaaccggact atgtcgttga gccggagctt ccgccggagc tcaagctgca
     4261 gctcgccctg cttccgttcg aatccgaggc gcagctgagg ggacacctga agaattccgg
     4321 caccgaagaa tccggaggca gcgtctccta tgtgccaagg gaaaaggaaa aggacgttca
     4381 gctcaaggcc gccattgccg ttcttcatgg cgagttcccg caaacgggga agaaggccat
     4441 agatcaggca cgggctgcgt cggatacaac acaccccgcg ctcggaacaa gcggcggctg
     4501 agttctagag cccgcttttg gtggaatcaa aagcgggctc tcagtttttg tttttgtcgt
     4561 gcgtctttac cggaaaaccg cttcgcactt ttcccgacgc gctccagcgt cgctttcaga
     4621 ggcgctgagg gagcgatgcg ttataacgtg ctctcttccg ctttctgaga cgaggccatg
     4681 agcggaatac gttctcttcg atttgcagtt ccgccagcgt tgtaaggatg caatcgtcgc
     4741 gcggctttcg catcacgatc ttcgacaacc gcacgcgcgg cgagtttcca atcgccttcg
     4801 gagcgtctcg cgccgcgtaa ctggaacctt cacccaaatt tgctgcggcc taacatcgat
     4861 taagaatttt ccgttcctta agctggcgga tttgcatggc ttggctctca tgcaaaatct
     4921 tgagctgagc aatctgctca ttcgcggata gcaggtcttt ttttagccgc tcgttttcgc
     4981 gttctaattc ttcgatgcgt tgttgttgtc tggtcatgca attggtttcc tgccgaagcg
     5041 ctgtagggca agagtgacgc cttccagcaa gctggaatac tttaccagaa actccggcaa
     5101 tcggcggtgt tttccgtaag tccttaactc ggcttaagga ctagggcctg catcagccga
     5161 ttagcgtctg cggtgcaagg gatgcaatcg gtgaagcggg tttcgcatca ccgtattcga
     5221 caatcgcacg cgcgcggata attcagaggc gcggttggag cgggtcgttg tggcggggga
     5281 gtgaggcaag cttgaagtaa tcggcgcatc gcttgccgga attgccgccg agtcgacgat
     5341 aaggtgatgg tgcgggagca tgacgtctgg ctgcgcaatc cctatattac ttgcatgaac
     5401 atcattgttt tcggacccac cggcggcacg ggccgcgctg tcatcgcgaa gatgctggcg
     5461 gatggccata cggttacagc ttttgcgcgc gatccctcga agctctcgcc cgcgccaggg
     5521 cttacgattg tcaaaggcga cgcgatgaac cccgctgacg tgaagcacgc catccccggt
     5581 cacgacaccg tcgtcataag cctcggtaac ccgcagctgg cgatcgccat atcgcttggt
     5641 gcgcgccgca caacgcccgc cgacatttgc gaaataggga cgcgcaatat cgtcgccgcg
     5701 ctcgggccga cctcacccgt tcgcgttctt gccgtgacct ctttcggcgt cggcgacacg
     5761 cgcgacaaga tgcagatagc tttcaagatt ttctcccagc ttttattgcg ggaagccatg
     5821 gccgacaagg aacgccagga agccgtgctg aaggcctcgg acctcgatta tgttctggtg
     5881 cagccggtcg ggcttacgga tacccccgcc tcgggcgatt gggttgccag cgcaaagggc
     5941 gaaacccgaa agatgcgcat gtcgcgcaag gacgtggccg cgttcatcgc cgaggacatc
     6001 gcgaacccgc tctatcaccg gcaaaccgtc gccctttcgg ggtaatgcga gccgccataa
     6061 gtcaacgacg cctttgccca tcgggcctat ataattactt gtatatttac ttataggtct
     6121 tttacaaaac gaaaccaatt ttaaccgttt gtacacccca atggatgccc ctacaggctt
     6181 ggaaacctcc atctgagtcg aacgctccga cggttgatat aagcgagctc tacgcagcgg
     6241 cgatgaggtc gaaatcgccc tgaagtaccg cgtcgagccg tccggtacgc cagcccgcaa
     6301 ccatgtcgcg gacaagcggc gaggcgccgt tccgatagcg gcgaacaagc gtgttcgagg
     6361 gcgcggagtg cgagagtgcg atggcaagtg ccgcaggaag cctgtttggc gggatttcgc
     6421 ccaaaggcgc agccgcaagc cggatgcgcg ctgtaacgcg gtcgacggat ttctcctccc
     6481 ccggcatttc caggacgtgc aaccccgctt cgtgcgagag cgtccgatag gccccaaaac
     6541 cgccgatgac cagcaaaggc ggctctttcg ccttcggggg aacctccgaa agctgcatgt
     6601 tccgcgaagc cgcccaggcg cgatacatcg ccaggagttg ttcgcaccat tcttccgggt
     6661 cgccgtggcg gtcccctccg ccttccagcg caggctcgac cgaaagaaca gcttcgatcg
     6721 gcgcagcctc atatacatcc ttgatgcctt cgccgatgag atggagctgc aacgcaagcc
     6781 gggcgacaag ctcctttgaa gacttgcctc cgctccccgt tcccctggcc aggcgcgcgc
     6841 ggaaggactc ggccgtttcc gatgcggcgc gcacgcggtc
//
LOCUS       DATMGX010000977         3010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_265946, whole genome shotgun sequence.
ACCESSION   DATMGX010000977 DATMGX010000000
VERSION     DATMGX010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265946"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VNR65_16025"
     CDS             <1..730
                     /locus_tag="VNR65_16025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWJ20226.1"
                     /translation="ADGRNSELRDMAGIRTTVWPSDKVGIVATVGIEIPHEGVARQHF
                     LPAGPFAILPMTGDRVSIVWTEEGRAAKQILEQDRETVLDELSRRVGPELGALTLLSE
                     PKAYPLAMTLARDFVRPRFVLVGDAAHGLHWIAGQGLNHGLKDVAALAETLIDAARLG
                     LDIGGLSVLKRYERWRRFDSTTSAFTAVAINTLFSNDSMALRMVRSTGLRSVDRLSRL
                     KNFFMKEAAGLTGDVPKLLRGDPV"
     gene            complement(731..943)
                     /locus_tag="VNR65_16030"
     CDS             complement(731..943)
                     /locus_tag="VNR65_16030"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HWJ20227.1"
                     /translation="MDVSETKDEIDPLLLQILVCPVTKEPLHWDKEAQELVSKAAGLA
                     YPVRKGIPIMLPEEARKLDEEQAGVA"
     gene            complement(1022..1699)
                     /locus_tag="VNR65_16035"
     CDS             complement(1022..1699)
                     /locus_tag="VNR65_16035"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWJ20228.1"
                     /translation="MGITDRYRSLADLPGHLPIFPLQGCILLPRSNLPLNIFEPRYLS
                     MVDDVIAGDRVIGIVQPLGAEEESPASKGHPLRQIGCAGRLSAFSETEDGRLLITLTG
                     ICRFDIIGETRTAKPYRLCDANFAPYLKDLERGYGQNAVDWPRFLEVLKTYLDARDLT
                     ADWDSIQRSPTELLINTLSMISPYGPEEKQALLEAPDLKARSEVLMALAEMEIAAPGS
                     GPGTSLH"
     gene            complement(1727..2641)
                     /gene="trxA"
                     /locus_tag="VNR65_16040"
     CDS             complement(1727..2641)
                     /gene="trxA"
                     /locus_tag="VNR65_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWJ20229.1"
                     /translation="MAISLFGGGAAKKNETAGDLIKDTTTQTFVADVINASRKVPVIV
                     DFWAPWCGPCKQLTPILEKVVKAAKGAVRLVKMNTDEYPDVAAQLGVQSIPAVFAFKN
                     GQPVDGFMGALPESQVRAFIERVMGPDAGGEAGLEDAQAAFAAGDVGGAASQFAAILQ
                     SDPENAEAVAGLAKCYIQTGDLNRAEQTLALVPPAKQTSESYRSAKAALDLAKKAGTK
                     SDIKGLEQAVTANPKDWDSRFKLAIALNARGQRREAMDHLFEIVRKDRAWNDDAARKQ
                     LVEFFDAWGAKDPLTLEGRQRLSSLLFA"
BASE COUNT          588 a          938 c          873 g          611 t
ORIGIN      
        1 agcggacggg cggaactccg agcttcgcga catggccgga atcaggacca cagtctggcc
       61 ctcggacaag gtcgggatcg tcgcaacagt cggaatcgaa atcccccatg aaggcgtagc
      121 gcgccagcat ttcctgccgg ccggtccctt tgccattctg cccatgaccg gcgaccgcgt
      181 ctccatcgtc tggacggaag agggccgcgc ggcgaaacaa atccttgagc aggaccgcga
      241 aacggtgctc gacgaactct cgcgccgcgt cggcccggag ttgggagcgc ttacgcttct
      301 gtcggagccg aaagcctatc ctctagcaat gacgctcgcg cgcgatttcg tccgcccacg
      361 ttttgtgctc gtgggcgatg cagcccacgg cctgcactgg atcgcgggac aaggcctcaa
      421 tcatggattg aaggatgtcg ccgccctggc cgaaaccttg atcgatgcgg cgcgtcttgg
      481 cctcgacatc ggcggcttga gcgttctcaa gcgatacgaa cgctggcggc gcttcgacag
      541 caccacatcc gccttcacag ccgttgcaat caacacgctg ttttcgaatg atagcatggc
      601 actacgcatg gttcgcagca cggggcttcg ctcggtggac aggctttccc gtctcaagaa
      661 tttcttcatg aaggaggcgg ccggattgac cggcgacgtt cctaagcttc tgaggggcga
      721 tccggtctga ctaagctacc ccggcctgct cctcgtcaag cttgcgcgct tcttcgggga
      781 gcatgatcgg aatgcccttg cggacgggat aggcaaggcc ggcggccttg ctgacaagct
      841 cctgggcttc cttgtcccag tgaagcggct ccttcgtcac cgggcatacc aaaatctgga
      901 ggaggagcgg gtcgatctcg tctttcgtct cggaaacgtc cattgcgctg aactctcttg
      961 ctaatgccgt ggctgatgcg gatgtgccga acaccgtttt gacagttccc ggcgtttcgc
     1021 gctagtgaag gcttgtccca ggcccggacc cgggcgctgc aatttccatc tccgccagcg
     1081 ccataagtac ctccgaacgc gcctttaaat cgggcgcttc caagagagcc tgcttttcct
     1141 ccggcccgta ggggctgatc atggaaaggg tgttgatgag cagttcggtg ggcgagcgct
     1201 ggatgctgtc ccagtccgcc gtcaagtctc gcgcatcgag ataggtcttc aagacctcca
     1261 gaaagcgggg ccaatccaca gcgttctgac cgtatccgcg ctccagatct ttcaggtatg
     1321 gagcgaaatt ggcgtcgcat agtcgatagg gcttggcggt tctcgtctca ccgatgatgt
     1381 cgaagcggca gatgccggtg agggtaatga gaagtcgccc gtcctcggtt tcggaaaagg
     1441 cggaaagcct ccccgcgcag ccgatctgac gcagcggatg ccccttcgac gccggtgact
     1501 cttcttccgc gccgagaggc tgcacaatgc cgatgacgcg atcgcccgca atgacatcat
     1561 ccaccatgct tagatagcgc ggctcgaaaa tgttgagagg caggttcgag cgcggaagca
     1621 ggatgcagcc ctggagcgga aagatgggca aatgtccagg aagatcggcc agcgatcggt
     1681 atctgtccgt gatccccaaa gtcgcctcct tcaccggaaa ccggcatcaa gcaaataaga
     1741 gcgaagagag acgttgccgc ccctccagcg tgagagggtc tttcgccccc caagcatcga
     1801 agaactcgac cagctgcttt ctggccgcat cgtcgttcca ggcgcggtcc ttccgcacga
     1861 tctcaaataa atggtccatg gcttcgcgcc gctggcctct tgcgttaagc gcgatggcga
     1921 gcttgaaccg ggaatcccaa tccttgggat tagcggtcac agcttgttcc aggcctttga
     1981 tatccgattt cgtccccgcc ttctttgcga ggtccagtgc agccttagcg ctgcgatagc
     2041 tctctgaagt ctgctttgcg ggtgggacca gagcgagcgt ctgctccgct ctattcagat
     2101 ccccggtttg aatgtagcat ttcgcaagcc ccgcgaccgc ctcggcgttc tccggatcgg
     2161 actgcaaaat cgccgcgaac tgcgacgctg cgccgcctac atcgccagcg gcgaaggccg
     2221 cctgggcgtc ttcaaggcct gcctcgccgc cagcatccgg gcccatcacg cgctcgatga
     2281 aggcgcgcac ctggctttcc ggcaaagcgc ccatgaaacc gtccacgggc tgtccgttct
     2341 tgaaggcgaa caccgcgggg atcgattgca cgccaagctg cgccgccacg tccggatact
     2401 cgtcggtatt catcttgacg aggcggaccg cgcccttcgc cgccttgacg accttctcaa
     2461 ggatcggcgt gagttgcttg cagggtccgc accacggcgc ccagaaatcc acgatcacgg
     2521 gcactttccg cgacgcattt ataacatccg ccacgaaagt ttgtgtcgtt gtgtctttga
     2581 tcaggtcgcc cgcagtttca tttttttttg cggcgccgcc gccgaagagc gagatggcca
     2641 tcagtgtgtc acccggatat atgcatgcga cattgggaat cttatgaatc agcggcctgc
     2701 cagaattcgc atcagcctgc ggcggagata gtcttcccgc cgccatcaga caagcgcaca
     2761 aattttcaac tacgcttggc gaagggatcg ccccgcgtca agaaaaagct aagaaacacc
     2821 ccgcatgccc cccaatccca tgatcagatg gcgtcctgaa gcgggcgctg ccctatctgg
     2881 aactcaccgg ttacgatagg agctgcagcg attttccaac tacggcgatc tacgcgcaga
     2941 agcataaatc cggcagtctg cataacccaa gaggctgcag tgcaattccg cttatgatcg
     3001 cccgcgcgcg
//
LOCUS       DATMGX010000978         2556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_341268, whole genome shotgun sequence.
ACCESSION   DATMGX010000978 DATMGX010000000
VERSION     DATMGX010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341268"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VNR65_16045"
     CDS             <1..414
                     /locus_tag="VNR65_16045"
                     /inference="COORDINATES: protein motif:HMM:NF036442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5372 family protein"
                     /protein_id="HWJ20230.1"
                     /translation="ARPPAICNSPRNAHAAAASAHLVRITHPFHPFSGRQLVCVGERH
                     NRSGKRLLLRVDDTTICSVPPQWTDIVAPDLEVLIGQGRALFRVADLVELARLVTRLS
                     SEGRSDRIVDCKDNSAAPVKQFVPQASKNMNGSRR"
     gene            498..965
                     /locus_tag="VNR65_16050"
     CDS             498..965
                     /locus_tag="VNR65_16050"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ20231.1"
                     /translation="MPARDDRKRSKAEALAEDGTLNPAPDKVGDPKFQEGGFFDPRDI
                     VQVKYEMLRRVSVEKTLVTHVSDQYGISRPTFYQAKADFDDGGIAGLVPQKRGPHGPY
                     KIQSEVLAFLIAQLAPGEPIRARELAKLVQKEFGIDVHPRTIERAVGGKKTPR"
     gene            962..1264
                     /locus_tag="VNR65_16055"
     CDS             962..1264
                     /locus_tag="VNR65_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20232.1"
                     /translation="MTDAEARSSTVSATLVAQYETLRSAMLGEALPPDARSWLIVFLR
                     HGMWEWARTLTLGTRGQEPLSVSPSSSAHPTEFGERRAVIHLLAAMAMTINHRRRS"
     gene            1261..>2556
                     /locus_tag="VNR65_16060"
     CDS             1261..>2556
                     /locus_tag="VNR65_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWJ20233.1"
                     /translation="MNQSLKVQSHHLERGAYLYIRQSSMRQVLENVESTKRQYALRGR
                     ATALGWRDDQITVIDSDQGESGASASWREGFQLLVTDVSMGRAGIVMGLEVSRLARNN
                     ADWHRLLEICALADTLILDEDGVYDPASFNDRLLLGLKGTMSEAELHVIKARLRGGIL
                     NKVRRGEYRCVLPTGFAYLESGNVVLDPDSQIRETLTYFFETFSRVGSACQTVKVFQD
                     NGLLFPSRIRNSATTIFQPLTTSTAIRTLNNPRYAGAYAYGRRHYRRAADGKKVLRKR
                     ECNDWLACIPNAHPGYITWEQFQQNLKTLETNGRGFEVARASSPREGTALLQGRAVCG
                     CCGRYLRTRYAARRQRLEAWYVCDRAHGSRGEPNCQSIAGAPVDEAIGELISAKMTPA
                     AVELALEIRKEIEARHEEADRLRLRAVERARIEADLAQRR"
BASE COUNT          508 a          767 c          777 g          504 t
ORIGIN      
        1 gcccgtcctc cagccatttg taactcacct cgaaatgcac acgctgccgc cgcttcggcg
       61 caccttgtcc gcataaccca tcccttccat cccttcagcg gccggcagct tgtctgcgtc
      121 ggcgaacgcc ataaccggtc cggcaagcga ctcctgctgc gtgtggacga cacaaccatt
      181 tgctccgttc cgccgcaatg gaccgacatc gttgcgccgg atttggaagt cctcatcgga
      241 caaggccggg cgctcttccg agttgcggac ctggtggaac tcgcccgtct tgtgacgcgc
      301 ctgtcctcgg aggggcgttc cgatcgcata gtggactgta aggataattc tgccgcacct
      361 gtaaagcaat ttgtgccgca ggcaagcaag aacatgaacg ggtctcgccg ttaagccgga
      421 ggcctgccct tgacagaggg cctagtagcg gcgtatttta aatgactttt tgggtgtcgg
      481 gtcgatcgga gttcaacgtg ccagcacgcg acgatcgcaa gcgctcgaag gccgaggccc
      541 tcgcggaaga cggcaccttg aacccggcgc ccgacaaggt cggcgatccg aagtttcagg
      601 aaggcggatt cttcgatccg cgggatattg tacaagtaaa gtacgagatg ttgcgccgcg
      661 tctctgtcga gaagactttg gtcacacacg tgtccgacca atatggcatc tctcgtccga
      721 ccttttacca ggccaaagcg gatttcgacg atgggggcat tgcgggcctg gtgccgcaga
      781 aacgtggtcc acacggccca tacaagattc agagcgaggt gctagcgttt ctcatcgcac
      841 agctggctcc tggcgagcca atccgggctc gtgaactggc gaagctggtt cagaaggaat
      901 tcggcatcga tgtgcatcca cgaacgatcg agcgcgcggt cggtggaaaa aaaacgccgc
      961 gatgacggac gccgaggcga ggtcctcgac agtttcggcg accctcgtgg cgcagtacga
     1021 gacgctgcgt tcggccatgc tcggcgaggc gctgccccca gacgcccgca gttggctgat
     1081 cgtctttctc cgtcacggca tgtgggagtg ggcccgtacc ctgacgctcg ggacccgcgg
     1141 ccaggagccg cttagcgttt ctccgtcctc gtcggcacac ccgacggagt tcggcgaacg
     1201 tcgcgctgtc attcacctgc tcgctgctat ggcaatgacg atcaatcatc ggagaaggtc
     1261 gtgaaccagt cactcaaggt ccaatcgcat catctcgaac gcggcgctta tctctacatc
     1321 cgacagtcat cgatgcgaca ggtccttgag aacgtcgaga gcacgaagcg acagtatgct
     1381 cttcgcggtc gcgcgaccgc gcttggctgg cgtgatgatc agatcactgt catcgacagc
     1441 gaccaaggtg aatccggcgc ctcggcgtcg tggcgcgaag gattccaact cttggtgacc
     1501 gatgtgagca tgggacgggc gggcatcgtc atgggtctgg aggtgtcccg cctggcaagg
     1561 aataacgccg attggcatcg cctgttggaa atctgcgccc tcgccgatac gctgattctc
     1621 gatgaggatg gcgtgtacga tccggccagc tttaacgacc ggctgttgct cggcctgaaa
     1681 ggaacgatga gtgaagccga actccatgtc atcaaagccc gattgcgcgg cgggattctg
     1741 aacaaggtgc gccgcggcga gtatcgctgc gtgcttccga ccggcttcgc gtaccttgag
     1801 tccggcaacg tagtgctcga tcccgattct cagatcaggg agacactcac ttacttcttc
     1861 gagacgttct cgcgcgtcgg ttccgcctgc cagacggtca aggtctttca ggacaacggc
     1921 cttctcttcc cctctcgaat ccgcaatagt gcaacgacga tttttcagcc gttgaccaca
     1981 tcgaccgcga tccggacgtt gaacaatccg cgttatgcgg gcgcctacgc gtacggtagg
     2041 cgccactatc ggcgcgccgc tgatgggaag aaggttctgc gaaaacgcga gtgcaatgac
     2101 tggcttgcct gcatcccgaa cgcccatccc ggctacataa cctgggagca atttcaacag
     2161 aacctcaaga ctctcgagac caatggccgc ggatttgaag tagcgcgtgc gtcgtcgccg
     2221 cgagagggca cggcgctact gcagggacgt gcggtgtgtg ggtgttgcgg cagatatctc
     2281 agaacccgat atgctgcgcg gcgccagagg ctggaggcct ggtacgtctg cgatcgtgca
     2341 cacggctctc gcggtgaacc gaactgccag tccattgcag gtgcgcccgt cgacgaagcc
     2401 atcggtgagt tgatcagcgc caagatgacg cccgccgcgg tcgaactggc tctggaaatt
     2461 cgaaaagaga tcgaggctcg acacgaagag gccgatcggc tccgtcttcg agctgttgaa
     2521 cgcgcacgga tcgaagcgga tctcgcgcaa cggcgg
//
LOCUS       DATMGX010000979         3577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_456225, whole genome shotgun sequence.
ACCESSION   DATMGX010000979 DATMGX010000000
VERSION     DATMGX010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456225"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1465
                     /locus_tag="VNR65_16065"
     CDS             308..1465
                     /locus_tag="VNR65_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ20234.1"
                     /translation="MDDFARRILLIENSQAVATQIESSLSNPGSFDLQRSEDLTSGLN
                     QLRSSSFDAVVLDLSLPDSAGIGTLVAVRNECPETPIVVLGEDENPSAGMRVLSEGAQ
                     DYVTKAAWNPVSLPRILRFAIERHNTGRPSVTAKDGRLLTFIGAKGGVGTTTVALNIA
                     AALAALDHPTIATEWCWPRGNFSLYLTEPPPTNLSAIGTLPPRAITASVLKKLLVTSS
                     HNLMLLHGPQAAGEFQYLNHAQAEAFVAGLLQIADFIVIDLPPHPSTVSRVAVRHSST
                     VVVVLERNPESVVLANETLSMIRSWAAGDIAITAVVVSKGGSATPMSLDDIRSGLRCG
                     VVGVVPPSTEPTPGFSKGLPITISRQGTLPADTLTDIAKRLASKQLELLAS"
     gene            1630..3054
                     /locus_tag="VNR65_16070"
     CDS             1630..3054
                     /locus_tag="VNR65_16070"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWJ20235.1"
                     /translation="MRTECRLRIVLYIAAVIFFAVSAKAQEPTAPSASPVPMQLAPAP
                     GQVVTLTPEAGFHNEPSIAVNPMNASQIVAAYQVPATVAFSRDGGSSWKVATGTAPSD
                     YRVSGDVSVTYDKHGAAILCYIAFDQLGTENYWAHGATRNGIFVRRSADGGATWDAQA
                     HAVLAQPTKPGIPFEDKPYIVADNTDGKYAGNLYVGWTEFRIDESVILFSRSTDGGLT
                     WPAPIEISTHHGLPRDDNGAVEGFTGAVAADGTLHVVWADGDSVVLASSHDGGKTFSK
                     SRAILKTAPLYFAVAELDRANGFPEIAIDPRHSRKGLLYVTWSDYRDGDIGVFCATSG
                     NGGRSWSDPVRVNSNPAHDGTDQFFQWLAVDPATGAANVIFYDRRLDPDNRKTTVTLA
                     RSVDGGRTFTNYAWTRDAFVTRRQEFLGDYIGIAALNNKVYGAWAEIPPPTAPKDKTE
                     PPPTSFRPHTVVRVGVADFGNAPH"
     gene            complement(3254..>3577)
                     /locus_tag="VNR65_16075"
     CDS             complement(3254..>3577)
                     /locus_tag="VNR65_16075"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HWJ20236.1"
                     /translation="YLVRQLRDHKAGIENEDLEGNGLVQYGRVCGELLSKGHARSGDA
                     CAISGYLGNSDRFDKAMVAFGAAYADQSTKDFDEFTRAIRAGKVPAAPAASPAAKQGH
                     RRAKT"
BASE COUNT          716 a         1067 c         1043 g          751 t
ORIGIN      
        1 atggggggtt gaattaagga aactctgaac aagtcgaagt tcgggtagag ccggccttca
       61 ggccggcgtc taagctgttg taaatactcc ggggacttca gccccgggaa acagaatgct
      121 gacttgctca gagcctcctt aggtgcgttt cagctctcat ttctccgtcc caggttgctg
      181 gtctgcacat aggggttatc ggcacaaccc gcgctgcggt ttagaggtgt tttttagggc
      241 ccctcttaca tactttccac gatccgccga tgttaatgac cagaacggcc gtacgaatcg
      301 agttgctatg gatgattttg cgaggcggat tctgctgatt gaaaattcgc aggccgttgc
      361 tactcagatc gaaagcagtc tgtccaaccc gggatccttc gatctgcagc gatcggaaga
      421 cctcacgtcg ggtctaaacc aattgcgcag ttcgtctttc gatgcagtgg tccttgacct
      481 gtcgctgccc gacagcgcgg gcataggtac cttagttgcc gtccggaatg aatgcccgga
      541 gacgcctatc gtagtgcttg gagaggatga aaatccgagc gcaggcatgc gggtgctcag
      601 cgagggggcg caggattacg tcaccaaggc ggcgtggaac ccagtatcgc tgccgaggat
      661 tctgcggttc gcgatagagc gccataacac tggccgtcct tcagttacag ccaaggatgg
      721 aaggctgttg acatttatcg gggcgaaagg cggagttggc accaccaccg tggcgttgaa
      781 cattgcggca gcgctggcgg cgctcgatca cccaacgatc gccacagaat ggtgctggcc
      841 tcgtgggaac ttctctctct acctcacaga accgccgccg acgaatctca gcgcgatcgg
      901 aacattgcca cctcgggcca ttaccgcatc ggtgttgaag aagctgctgg tcaccagctc
      961 gcacaacctg atgttgctac acggcccgca agctgccggt gagttccagt atttgaacca
     1021 tgcccaggcc gaagcatttg tcgcgggcct tttgcaaatc gcggatttca ttgtgatcga
     1081 tctcccgcct catcccagca cggtcagccg cgtggctgtt cgccactcgt ccaccgttgt
     1141 cgtggtactg gaacgtaatc ccgagtctgt cgtcctagcg aatgagacgc tctccatgat
     1201 tcgttcgtgg gccgctggcg atatcgcgat aacagcggtg gtggtaagta agggaggatc
     1261 ggccactcca atgtcactgg acgacattcg atcggggctg cgctgcggtg tggttggggt
     1321 tgttccaccc agcacggagc ctacgcccgg attctccaag gggctcccaa ttacgatctc
     1381 gcggcaaggc acgctcccgg cggacactct gaccgacatc gcaaaacgac tggcaagcaa
     1441 gcaattggaa ttgctggcaa gttgagtgcc tcgctatcct gggcctgttc aggccatcga
     1501 agctcgagcc cgaaagtctg ctcaccgaga tacaagactt gttctccttc tctttgaaat
     1561 ccaacggcat tccgtgtgta atcggaactc agcctgcaag caatgccgca agcaaaagat
     1621 cgagccccga tgagaaccga atgtcgtcta cgtatcgttc tgtatattgc agccgtaatt
     1681 ttctttgctg ttagcgccaa ggcacaagag ccgacggcgc catcggccag cccagtgccg
     1741 atgcaactcg ctcccgctcc gggacaagtg gtgaccctga cacctgaggc cggtttccat
     1801 aacgagccct caattgcggt gaaccccatg aatgcttcgc agatcgtagc cgcatatcag
     1861 gttccggcga cggtggcctt ctcgcgagat ggcggtagct cgtggaaagt cgcgactggt
     1921 actgccccct ctgactatcg cgtgtcaggc gacgtctccg ttacctacga caagcacgga
     1981 gccgcaatcc tgtgctacat cgcgttcgac caactcggca ccgagaacta ctgggcgcat
     2041 ggcgccacgc gcaacggcat cttcgtgcgc cgctcggcgg atggcggcgc tacctgggac
     2101 gcccaagccc acgccgtctt agcgcaaccg acgaagcccg gcattccctt cgaggacaag
     2161 ccgtacatcg tcgctgacaa taccgacggc aagtacgctg gcaacctcta cgtgggctgg
     2221 accgagttcc gcatcgacga gtccgtcatt ctattttcgc gctcaaccga tggcggtctg
     2281 acgtggccgg ctccgatcga gatcagcact catcatggcc tgccgcgcga tgacaatggc
     2341 gcggtcgaag gtttcaccgg ggccgtcgcg gcggacggta ccctgcatgt agtttgggcg
     2401 gatggcgatt ccgtcgtgct cgcgtcctcg catgatggcg gcaagacatt ctccaagagc
     2461 cgcgccatac tcaaaacggc tccgctgtac tttgcggtag cggaactcga ccgggcgaac
     2521 ggctttccgg agattgcgat tgacccgcgt catagccgca agggactcct ctatgtcacc
     2581 tggagcgatt atcgcgacgg tgacatcggc gtgttctgcg ccacttccgg caatggaggc
     2641 aggtcctgga gcgatccggt gcgcgtgaac tcgaatcctg cccatgacgg caccgatcag
     2701 ttcttccaat ggctggcggt cgatcccgcg actggcgcgg caaacgtcat cttctatgac
     2761 cggcgcctcg atccagacaa tcggaaaacc accgtgaccc tcgcgcgttc ggtggatggc
     2821 ggccgcacat tcacgaatta tgcgtggacg cgtgatgcat tcgtcacccg ccggcaggaa
     2881 tttctcggag actacattgg aattgcggcc ctgaacaaca aggtgtacgg cgcgtgggcg
     2941 gagattccgc cgcccactgc gccgaaggac aagaccgagc caccgccgac atcgttccgt
     3001 cctcataccg tggttcgagt gggcgtggcc gactttggca atgcgcccca ctagagcatt
     3061 tccacacttg gtgtataggg tggagcaccg cttcagcggt gcagttagga ttcggcaatg
     3121 agacagcttt agccgctgag gtgacggaaa cattacctca ggcgctaaag cgcgatccaa
     3181 gaattgacgg cagttatgca cgcctgaagg cgtgctccac cccatcgcct atacaccaga
     3241 tgtcatcgcg aacctaggtt tttgctcttc ggtgaccttg tttggctgcg ggagacgccg
     3301 caggggccgc gggaactttg ccggcgcgga tggcccgcgt gaactcgtcg aagtccttcg
     3361 tgctctggtc ggcgtaagcg gcgccgaagg caaccatggc cttatcgaac ctgtcgctgt
     3421 tcccaagata tccggagatt gcgcatgcgt cgcctgagcg agcgtgcccc ttggaaagca
     3481 actcgccaca aacgcgcccg tactgtacca gtccgttgcc ttcgagatct tcgttctcaa
     3541 tgccggcctt gtggtcgcgc aactggcgga ccaggta
//
LOCUS       DATMGX010000980         3148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_341449, whole genome shotgun sequence.
ACCESSION   DATMGX010000980 DATMGX010000000
VERSION     DATMGX010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341449"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..482
                     /locus_tag="VNR65_16080"
     CDS             246..482
                     /locus_tag="VNR65_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20237.1"
                     /translation="MKKAFVCMVTAVFIALSGAYAGERPLDAGLGVVSGAVFFGPVGA
                     IAGGVIGYTKGPAISRSMGLSGHHHHRQHSRGGR"
     gene            complement(609..2165)
                     /locus_tag="VNR65_16085"
     CDS             complement(609..2165)
                     /locus_tag="VNR65_16085"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HWJ20238.1"
                     /translation="MIDRRTLLIGSAALSLAASTRAMAAQPESIFAGPQIPFSFEKLI
                     ASAKELSERPYQRPVVHDPELLERLDFDAYQQIKFRPELAIWGQGEGSYPIELFHVGR
                     YFKDPVRIFVVSAAGVAQEVGYSPDLFTYGKSEFAKALPADTGFAGFRVLAAPGMPDW
                     LAFLGASYFRSPAETGQYGLSSRGLAIDCAMPTPEEFPRFSQFWLEPMAGRRGVIINA
                     LLDSQRITGAYRIETTHDGFALMNVTAHLFPRADIERVGIGPLTSMYWYGKPNRRTGT
                     DWRPEIHDSDGLSVWTGAGERIWRPINNPLSVQTSSFFDQRPKGFGLLQRERDFRDYE
                     DDGAFYHKRPSVWVEPIGDWGEGAVQLVEIPTSDEIHDNIVAYWKPRQPYRAGEHHEV
                     QYKLHWRLDEPYPAPLARVVATRVGTGGIPGQPRPKGMVKYVVDFRGGRLSELTKRGD
                     IDVVVTAASGTVERYAVYPVVDTDLWRVMFDFTAASEDPVDLRVYLKKDNDALSETWL
                     FQHLQSLSTM"
     gene            2408..>3148
                     /locus_tag="VNR65_16090"
     CDS             2408..>3148
                     /locus_tag="VNR65_16090"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HWJ20239.1"
                     /translation="MHIRHPYLLFLGDVQDQLAAKTAQGIVDWRPDWCLGQLRMPGCQ
                     ADVGIPDMTIREAVKAGARTLIVGVANAGGILPEQWTAPIVEALGSGLDVAAGLHMKL
                     AERPSVADAANRYGRKLFDVRQSDERFETGKGTKRAGMRLLTVGTDCSVGKKYTALAL
                     EREMRAGGFEVDYKATGQTGVFISGRGVAIDAVVADFIAGAVEWLSPAAEPHHWDLIE
                     GQGSMFHPSFAGVPLGLLHGAQPDAFVVC"
BASE COUNT          622 a          909 c          962 g          655 t
ORIGIN      
        1 aaagctcagc cgtcccaaga tacaatccgg gatcgtaacg aattaaagcg ccaacctttt
       61 tgcgatcccg gcacgcgctt cgcttggccg ggatgacggc ggcggcgatc cgttggcgcg
      121 tccgcgaaat cctgcttagg caaagctcat ttgatgatat gggacgaacg ctctgcggtt
      181 ggtattcagc caagagactg ccgcaattga cgctatcctg ccgcgcgggg cagaagggaa
      241 gccgtatgaa gaaagcattc gtttgcatgg tgacggcagt gttcattgca ttgtctggcg
      301 catatgccgg tgagcgtccg ctcgacgcgg ggctgggcgt tgtatcggga gccgtgttct
      361 tcgggccggt tggcgccata gcgggcggtg tgattggtta cacgaagggg ccggccattt
      421 cgcgctctat gggcctgagc gggcaccatc atcaccgcca gcattcgagg ggaggccggt
      481 agttctccga tgaactgccc ggccgacttc tgccgccaat ctggggccag caagcgctcg
      541 tggtccgatt ccgacatttg catccgtttg cagcacgccc gagagcaaat gtcggaatca
      601 aaaaggaact acatcgtcga gagcgattgc agatgctgaa acagccacgt ctcgctcagc
      661 gcgtcattgt cctttttcag ataaacgcga agatcgactg ggtcttcgga agccgcggta
      721 aagtcgaaca tcacacgcca cagatccgta tcgacaacgg ggtaaaccgc ataccgttca
      781 actgtgcctg atgcagcagt tacgacgacg tcgatgtctc cgcgcttcgt aagttcactg
      841 agccggccgc ctcggaagtc cacgacatat ttgaccatgc ccttgggcct tggttgacct
      901 ggaatgccgc ccgtgccaac ccttgtggcg acaacgcgcg ccagcggagc cggataaggc
      961 tcgtccagtc gccaatggag cttgtactgc acctcgtgat gctcgcccgc cctgtaaggc
     1021 tgccgcggct tccagtaggc gacaatatta tcgtggatct catcgctcgt tggaatctcc
     1081 acgagttgca cagcgccttc tccccagtca ccgatgggtt cgacccagac gctcgggcgc
     1141 ttatggtaga atgctccgtc gtcctcgtag tcgcggaagt cgcgctcgcg ctgcagaaga
     1201 ccaaagcctt tcggacgctg atcgaaaaat gaactggtct gtacgctgag cgggttgttg
     1261 atgggccgcc agatccgctc gccggctcct gtccaaacgc tcagaccatc gctatcgtga
     1321 atttcagggc gccagtccgt tcccgtcctg cggttcggct taccgtacca gtacatgctg
     1381 gtgagaggcc cgatgccgac gcgctcaatg tccgccctcg gaaagagatg ggccgtcacg
     1441 ttcatcagcg caaagccatc atgcgttgtc tcgatgcggt aagcgccggt gatgcgctgg
     1501 gaatccagca gagcattgat gatgacgccg cgcctgcccg ccatcggttc cagccagaac
     1561 tgcgagaagc gagggaattc ctctggcgtc ggcatggcgc agtcgatggc gagaccgcgt
     1621 gaggaaagtc cgtattgccc tgtttcggcc gggctgcgga aataggatgc accaagaaat
     1681 gcaagccagt ccggcatgcc cggcgcagcg agaacgcgaa agcctgcaaa cccggtgtcc
     1741 gccggaagcg ctttcgcgaa ttcgcttttc ccataggtga agaggtcggg actatagccg
     1801 acctcctgcg ccacccctgc ggcggagacg acgaagatgc ggaccgggtc cttgaaatac
     1861 cgcccgacgt ggaaaagctc gatcggataa gatccttcgc cctgccccca gatcgccagc
     1921 tccggccgga acttgatctg ctggtaggca tcgaaatcga gccgctcgag cagttccgga
     1981 tcgtgcacga ccggcctctg ataaggcctt tcgctaagtt ctttggcaga ggcgatgagc
     2041 ttctcaaatg aaaaaggaat ctgtgggccg gcaaagatgc tttcgggctg cgcggccatc
     2101 gctcttgtgc ttgccgcgag actcagcgct gccgatccga tcagcaatgt ccgacgatca
     2161 atcatttggc ctctccacaa attggtctca cggcttcggt tctggtaaag aaatgcgtat
     2221 tacaagtatg aaattaaagc tcgacctttt cgggaagctt gcgcggcaat ccccaacgtg
     2281 cgctcgccgc aagcgtcttt tataagggcc gcaatgctgt gctcaagcac tcgcaaaggg
     2341 ttcaaagggt ggtgtgagaa gaactttttg gctattatcc gtctgccatc ctggagcccc
     2401 catcatcatg catatccgcc atccctacct cctctttctt ggtgacgttc aagatcaact
     2461 tgcggccaag acggcgcaag ggattgtgga ttggcggccc gactggtgcc tggggcaatt
     2521 gcggatgccc ggatgccagg cggatgtggg cattcccgat atgaccatac gcgaggcggt
     2581 gaaggcgggc gcgcgtacct tgatcgttgg tgttgcaaat gcaggcggca ttcttcccga
     2641 gcaatggacg gctcccatcg tggaagcgct ggggtcggga ctcgatgtcg cagccggtct
     2701 tcatatgaag cttgccgaga gaccgtccgt cgcggacgct gcgaatcgct atggccggaa
     2761 gctttttgat gtgcgtcagt ccgacgagcg cttcgagacc gggaagggta cgaaacgtgc
     2821 gggcatgagg ctcttgaccg ttggtaccga ctgctctgtg ggcaagaagt acacagccct
     2881 ggcactcgag cgcgaaatgc gcgccggggg cttcgaagtc gactataagg caacggggca
     2941 gaccggcgtg ttcatttccg gccggggcgt cgccatcgac gctgtggtgg cagacttcat
     3001 cgcgggcgca gtggaatggc tttcgccggc ggccgaaccg catcattggg acctgatcga
     3061 gggtcagggc tcgatgttcc atccctcttt cgcgggcgtg ccgctggggc ttctgcatgg
     3121 cgcacagcct gatgcgttcg tggtctgc
//
LOCUS       DATMGX010000981         3301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_340074, whole genome shotgun sequence.
ACCESSION   DATMGX010000981 DATMGX010000000
VERSION     DATMGX010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340074"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..954)
                     /locus_tag="VNR65_16095"
                     /pseudo
     CDS             complement(1..954)
                     /locus_tag="VNR65_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611382.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1006..1230)
                     /locus_tag="VNR65_16100"
                     /pseudo
     CDS             complement(1006..1230)
                     /locus_tag="VNR65_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            complement(2531..2743)
                     /locus_tag="VNR65_16105"
     CDS             complement(2531..2743)
                     /locus_tag="VNR65_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWJ20240.1"
                     /translation="MSMARGTVKWFNPTKGYGFIQPASGGGKDIFVHISAVEKAGLST
                     LNEGQTVEYEEVPNKGKTSAENLKIV"
BASE COUNT          700 a          950 c          875 g          776 t
ORIGIN      
        1 ctagatggtg agccgaaggc gcgaatgtct ggggtgccgg cggccgatat ccgagcgctg
       61 agtgcggccg gatcgtgttg tagtgccttc gccattgctc gatgacgact tgtgcttctt
      121 tcaggctgta gaagatttct ccgttcagca gctcatcgcg tagtttaccg ttgaaggatt
      181 cgcagtagcc gttctcccag gggctgcctg gttcgatgaa cagggtcttg gccccaactt
      241 gtgtcagcca gttgcgcacg accttcgcag tcatctccgc tccattgtcc gaccggatgt
      301 gctcaggaac gccgcgcatc aacatgacgt cggccatggt ctcaagcacg ccgaagctgt
      361 tgatccggcg tgccaccctg atggctaggc attcgcgggt atactcatcg atcaacgtca
      421 tcagccgaag cttgcggcca tcgtgggtct gggcctccac gaagtcatag ctccacacgt
      481 gattgcgccg ctcaggcctc agccggacgc aggatccatc attaagccag agcctgcctc
      541 gcggcctttg tttctggggt actttaagcc cctcacggcg ccagatccgc tgcacgcgat
      601 cacaacccac atcccacccg gcctcatcca gcaatgactt gatccgacga tagccgtatc
      661 ggccgtactg cgatgccaac gcaataatgg cctgcgtcaa tgcatcttca tcagcacgaa
      721 ctagggtggt gtaacgctgc gttccgcgtg gctgaccgac catccggcag gcatggcgtt
      781 ccgagagacc gtactttccc cgaatgcgct caacagcctg ccggcgccgt tcggggctta
      841 caagtttccc gaggcaacgt ccttgagcac ctgcttctca agcgacagat cagcaacaag
      901 acgcttcagg cggacattct cgcgttccag gtccttcatc cgcttcgcct ggtcagccac
      961 ggctgtcgcc atgcgcagtg gctggccggt aaggcaccct tcgccgcaga attcatcggg
     1021 actcatgacc gcaacgacgg cttccttccc ttgctcagaa aggacagtga gcttgacctt
     1081 gcctttttgt atgtagaaaa ccgcatccgc aggagtgcct tgcgcgaaaa taacttcatc
     1141 tacccgataa ttggatattg ttcggccgcc attcacggtg gcaagaaata ccttcgggtc
     1201 aaacgggatt gctttggctt cctttcccat gagcggctcg ctttatagtt gcaatagaat
     1261 cagactactc ttttactgcc tacaacgcca gagttggatt tgacgctact aacgcggaat
     1321 cgtgcagact ttggctcgcc tagaaaataa cgcggcgaat tggctttaat tggctctcac
     1381 agcgcttggg cgagctgttg cggaattgac gcggcttgca cggccttagt cgggaacgtc
     1441 gcgtgagtga ccgacacggt cgttccctct ggggttgcca aggtttcaac cttggcaccg
     1501 agttgttgcg cgagcgcctt gatgattcgc ggtctgcggg cgctaccgag ctgcgttcac
     1561 gaagcgttct cgaattccgg tccgtactta taggccggtg ggtactggcc cctttcgata
     1621 ccccacttaa atcaatgttc ccttttgaat ttttgagtgg tcccttttag ttctcggtaa
     1681 tcaccgcccc ttatggtttt accgttcggc agaatgtctg agccccgggc gacagggagg
     1741 cgctggcccg actccgcgtg ccggggcttc ttttccgaag gttgggggat cgagtgtcct
     1801 gcattgcctg ttcactccgg ccgaagaatg gccattggtt cgtcgaactc gaaaaaataa
     1861 ttcacggccc atatctgacc gaagaaatag cgttcggcgt cgctgcatcc gaagcgaaaa
     1921 ggctgcgagg gcaaggatgg gactccagaa tttccatttt tgaaaaccat ggcaatatcc
     1981 gcaacgagta ttgcctttgc accgccttta agaaggcgcg gggagctcgg cgagctaccc
     2041 cgcacaaccc gcgcttcgtt tacgtcgatt aaggccgcct cagttggcgg cctctttcat
     2101 ttcaagcata gtgtacaatg tcagctattg gcacctagcg gacaatccga ccgcacccac
     2161 ttttgtccgc ttctggcaca tcgcgtcatt tcgccgcgct gcaaaatttg tcgcctattg
     2221 gtctaacagc ggacaacgga aggcgctcat tcctcaccca ttgaccgccg agtcacggtt
     2281 ctcgatcatg aatcttacca cgtggctcaa tcacgaataa ataaacacaa gcgacagcca
     2341 gagctattac aagaagcaca atgataatct cgctcatgat ccgaaacccc atttctgatg
     2401 aggagacaaa gacgattaac tgcttctagt ttcaagcaag ccgcacaacc ccacggattg
     2461 tcaatgatgt cgatcagaca gcgttattga cgcatctaaa tagacgaacg gaccgacggc
     2521 tccttgccgg tcacacgatc tttagattct ctgccgacgt cttgcctttg ttgggtactt
     2581 cttcgtactc aacagtctgc ccctcattga gggtgctcaa gccggccttt tcaacggccg
     2641 agatgtgaac gaaaatgtcc ttcccacccc cgctcgccgg ttgaataaat ccgtatcctt
     2701 tggtcgggtt gaaccacttc acggtgcctc tggccatact caaactccga tgttgacggg
     2761 ggatgataag gccaaaacgc aaagatgcca aacgccgccg ctatgtgtac cgaatcgatg
     2821 ctcgcccggt tcgacgcggt gatcaaataa ggtctgccgg tggcagatat tggcacttag
     2881 cggacattcg ctgcgtcgca atagtttgtc cgcttttgga ccaaagcgga caaaggcagg
     2941 tttttggttt gcgatggttt gtccgctaat gactaagagc ggacaaaggg cggcccggag
     3001 gctgaatagc tccgcagcgt ttgacccatc tgcgacattg gctagaacgg ttcgcggaac
     3061 gagagacata cagctcgcga aaacctcagc tcatgccttc actgcaaggc gtaagattca
     3121 tacccatgag cggctacacg atatcgccag tagaagcaga cattgatcgg cacgggcgct
     3181 gaatggatcg gtagcgatga cccatttgcg acaatcgggt gtgtgctggg cctacaaggg
     3241 cctgtccgga cgcttttggt aaccgcatcg gctctctcct gttcgcggtt aacaggtctg
     3301 t
//
LOCUS       DATMGX010000982         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_120978, whole genome shotgun sequence.
ACCESSION   DATMGX010000982 DATMGX010000000
VERSION     DATMGX010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120978"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..441)
                     /locus_tag="VNR65_16110"
     CDS             complement(67..441)
                     /locus_tag="VNR65_16110"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWJ20241.1"
                     /translation="MADNPYEIPHDLRAVLDQNVQQAHAAYEQISNLVTNTIRTWMTV
                     VPANPMLTGFQDIQNCAMDFAKENAESVFALAGKISNAKSPQEVLALQTQFTQDLMRV
                     LMNQTRDLYTLTGEAIQKPRRG"
     gene            complement(675..1739)
                     /locus_tag="VNR65_16115"
     CDS             complement(675..1739)
                     /locus_tag="VNR65_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20242.1"
                     /translation="MRRERIIKAVNSLREALHAAQIRELLRVARTGQQADTVNRTQRI
                     LLAYNEFTRHYQQFGDEEKDLMTFFGLTPLLDISFWSGLIDGEQAVNRKLISDVDVGA
                     YNVIFVMPKLRELLTRETDKGELIIADSQGTEREIKRVRVLIAEKERSLTDPQIVINV
                     IRSMDELYSSLSALHIDAGVNLAIGSIDSGSAKSFDFFGASPIMEDINTLLINVWDRI
                     KYAKEDNFRYQIEVAMMAAGFVNRIKDAQGQQMIGEEQAQRTTRIVAKSIETLFRSGA
                     YTEEMDAPREIRASQVLTPRTQLIEYKHEERAAEVKREEQRPALEPIAPADAAPSVPK
                     AAEFLRDLKDQLEMKEPAKT"
     gene            1815..>2195
                     /locus_tag="VNR65_16120"
     CDS             1815..>2195
                     /locus_tag="VNR65_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HWJ20243.1"
                     /translation="MPALDDPLEDGKPNSERIAKVIARSGLCSRRDAEVLIGEKRVSV
                     NGTVIESAALDVSPGDKVLVDGRPLALRQPPRLWRYHKPKGRVTTHKDPEGRPTVFEA
                     LPEELPRMISVGRLDYNTEGLLLLT"
BASE COUNT          426 a          643 c          634 g          492 t
ORIGIN      
        1 cttgtggcgc acagctatgg cgcgacccat cgagaagacg cctcgccgga ctcgctacgg
       61 gttttttcag ccgcgtcgcg gcttctggat cgcttcgccg gtcaacgtgt agaggtcccg
      121 cgtctggttc atgaggaccc gcataaggtc ttgagtgaac tgggtttgaa gagccagaac
      181 ctcttgggga ctcttagcgt tgctaatctt gccggcgagc gcaaaaacgg actccgcatt
      241 ttctttggca aaatccatgg cgcagttttg gatatcctga aagcctgtca gcatcggatt
      301 tgcgggcacc acagtcatcc aagtgcggat ggtgttggtc accaggtttg agatctgttc
      361 atacgctgcg tgtgcttgct gcacgttctg gtccaacacg gcacgcaagt catgcggtat
      421 ctcgtacgga ttgtcggcca aatatcttcc tcccaatcat catgccgtta ggagcaagtg
      481 tagcggtgca caggtataat tgccaatgcg tcgaatagtt cgcccgagac gcgtctgtac
      541 caagcgacgc ttgcaggtcg aagctgcaac tccataagcg ttggccggac catctcggcg
      601 acggttggcc cgacccggaa tagctacaga cgctcccgga aggcccgctc cggccttaag
      661 agccgagaac ttggctaagt cttggcgggc tccttcattt ccagctgatc tttgagatcg
      721 cgaaggaatt cggcggcctt gggtaccgaa ggcgctgcat cagcgggcgc tataggctca
      781 agcgctggcc gctgttcctc gcgcttcact tctgccgctc gttcctcatg cttatactcg
      841 atcagctgcg tcctcggggt gagaacctgg ctcgccctga tttctcgagg tgcatccatc
      901 tcctcggtgt aagcgcccga gcggaacagc gtctcgatcg acttcgccac gatgcgcgtg
      961 gttcgttggg cctgctcctc gccaatcatt tgctgaccct gcgcgtcctt gatccggttc
     1021 acaaaccctg ccgccatcat cgcgacctcg atctggtaac ggaaattgtc ttccttcgca
     1081 tatttgatgc gatcccagac attgatcaac agcgtgttga tgtcctccat gatggggctg
     1141 gcgccgaaaa agtcgaagga cttggcgctg cccgagtcga tggagccgat agcaaggttc
     1201 acccccgcat cgatatgcaa ggccgagagc gaggaataca gctcgtccat ggagcggatc
     1261 acgttgatga cgatctgcgg gtcggtcagc gaccgctcct tctccgcgat cagcacccgg
     1321 acgcgcttga tctcgcgctc cgttccctgg ctgtcggcga tgatgagttc gcctttgtcg
     1381 gtttcgcgcg tcaaaagctc gcgcagcttc ggcatgacga aaatcacgtt gtaggcgccg
     1441 acatccacgt ccgaaatgag cttccggttc acggcctgct cgccgtcgat gagccccgac
     1501 caaaagctga tgtcgagcag cggcgtcagc ccgaagaacg tcatcaggtc tttttcttcg
     1561 tcgccgaact gctgatagtg gcgcgtgaac tcattatatg cgagaaggat gcgctgcgtg
     1621 cggttcacgg tgtccgcttg ctgcccggtc ctggcgacgc gcaataactc gcgaatttgc
     1681 gccgcatgaa gggcctcgcg caaactgttc acggctttga taattctctc gcgccgcatg
     1741 tgtggttctc cttccccctt agcccgaatc acttcgcgct tcgcggatgc tagctctttt
     1801 tattggtaat gatcatgcct gcacttgacg accccctgga agatgggaag ccgaacagcg
     1861 aacggattgc aaaagtcatt gcaagatcag ggctttgctc gagacgggac gccgaagtct
     1921 tgattgggga aaagcgcgta tcggtgaacg ggacggtcat agaaagcgcg gcgctcgacg
     1981 tttcgccggg cgataaagtg cttgtcgacg gcagaccgct cgcgttgcgg cagccgcccc
     2041 ggctctggcg ctatcacaag ccgaagggca gggtgacgac gcataaagac cccgagggaa
     2101 ggccgacggt cttcgaagca cttccggaag aattgccccg aatgatttcg gtcggccggc
     2161 tcgactacaa cacggaaggg ctcctgctcc tcaca
//
LOCUS       DATMGX010000983         3707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_173409, whole genome shotgun sequence.
ACCESSION   DATMGX010000983 DATMGX010000000
VERSION     DATMGX010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173409"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2327)
                     /locus_tag="VNR65_16125"
     CDS             complement(<1..2327)
                     /locus_tag="VNR65_16125"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWJ20244.1"
                     /translation="MTKPIFFDPNNRRAGHISRAAWALAIATSVAGLIFLSSLFIFRS
                     FPETLQTAQSQRYPLLNDVAKVPHLLPSVRNLARKAQATKKQFPHSVQPPAGALAKAK
                     GLKNPGERREKPLTVGFYANWDDSSFSSLKNNIQNLDWVVPSWLYLQGSSMDLKVTLD
                     QKSLDMIRREKPGAAILAMIQNSSITKWDGPNLGRLLADPERRRERLNSVLAFIEEHK
                     LQGLVIDFEQIPDDAHKDTLAFLSEAHAIFKAKGLIIAVAAPFDDPHWNYKAYAKACD
                     YLILMGYDEHWADSQPGPVASQSWFSTRLGARMRDLDPAHTIVAIANYGYDWIVGGHD
                     AEELTFQEAILAARDSKVSIDLDPDTLNPRFSYLEDGSTHRVWFLDAVTAYNHLRASD
                     KYRPAGYALWRLGAEDPSLWQVLPRNYGAPPPASLESINPGTEVDFIGRGELLQIAAE
                     PSAGSRKIKVDDANATITGETYTKMPASFVIRRTGEMPGKIALTFDDGPSPDWTPKIL
                     DILKEKGVHATFFIIGENGAAYPRLVQRILAEGHDLGNHTFTHPNLGETSNSVADVEL
                     NATQRLIEALTGRSTRLFRPPYFGDAEPTSAEEIAPMQEAEKLGYLTVGLKVDPDDWQ
                     RPTPEQIIERVVAQATDPDPEKRGQVVLLHDAGGDRAPTVASLPKLIDTLRAKGFDFA
                     TTSELARWTRDQAMPPVPANEASPVVNRYVFFTFSWLQGAVTTLFLAAIGLGLARLAA
                     LCGLALLGRARAKRRGVPSGADELSVSVLIPAFNE"
     gene            complement(2526..>3707)
                     /locus_tag="VNR65_16130"
     CDS             complement(2526..>3707)
                     /locus_tag="VNR65_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20245.1"
                     /translation="AYLKKLLRRYQLVTVRKDTDRLVLVVEPLYVRAEDLKIEIHDAA
                     GEPVRGCELALDVSADRRLAEGWGKLGEKERLHGLEFAETDSHYALSLPVDIEPNELL
                     ISTAEPGNVARLSSVGSGCVLEARPWVTAEELRSGKIVRALKGAGQTLIAVFSTDSTF
                     AGSLGGAAAADGFWSDALRLVNVVSTAAWEKKVLSRAQAPGVSPETGKLQVEVQGGAL
                     AGDGSRGASLKILSKGSQEPPGPASIFQVQPIERYLLDIALKLIREDASIAPQYSVKQ
                     EALILVTGSVNDTGSYFCQHSVRRDKSPWSSPPWVRHARKIFALEVWTQGSADDMLKG
                     SRIKPAQNAPDGIYICNIQGPEGDKIALYGIVPKVISDANTRAKVFSYLTGQANSFLK
                     P"
BASE COUNT          743 a         1087 c         1100 g          777 t
ORIGIN      
        1 tcttcattga aggccgggat caggacagaa acagagagtt cgtccgcgcc ggatggaacg
       61 ccgcgcctct tcgcccgcgc acggcccaat agcgcgagac cgcaaagggc cgcgagcctc
      121 gccaagccga gcccgatggc agcgaggaac aacgtcgtca ccgccccctg cagccagctg
      181 aacgtaaaga agacataacg gttgacgacc ggggaagcct cattcgcagg gacgggaggc
      241 atggcctgat cccttgtcca tcgcgccaac tccgaagtcg tcgcgaagtc gaaacccttg
      301 gccctgagcg tatcgatcag tttcggcagt gaggcaacgg tgggggcgcg atcgccgccc
      361 gcatcatgca acagcacaac ttgaccgcgc ttttcgggat cggggtccgt tgcttgggcg
      421 acaacgcgct cgatgatctg ctccggcgtc ggcctctgcc agtcgtcggg atcgaccttg
      481 agcccaacgg tcaggtagcc caacttctcg gcttcctgca tcggcgcgat ttcctcggcg
      541 ctagtgggct cggcatcacc gaaatagggt ggccggaaaa gccgcgtaga ccgccccgtc
      601 aaagcttcga tcaagcgctg ggttgcattg agttcgacgt ccgcgacgct gttagacgtc
      661 tccccgagat tgggatgagt gaacgtgtga ttgccgagat cgtgcccttc ggcgagtatt
      721 ctttgaacga gtcttggata ggcggcgccg ttctcgccga taataaagaa ggtcgcgtgc
      781 acgcccttct ccttgaggat gtccaggatt ttgggcgtcc agtccgggct cggtccatca
      841 tcgaacgtca gcgcgatttt gcccggcatt tctcctgtcc gccggatcac gaacgaggcc
      901 ggcatcttcg tataggtctc ccccgtaatc gtggcgttcg cgtcgtcgac tttgatcttc
      961 cgcgagccgg ctgagggttc agccgcaatc tgcagaagct cgccgcgtcc aatgaaatcg
     1021 acctctgtgc cgggattgat cgattcgagc gaggctggcg gcggtgcgcc atagtttcgg
     1081 ggcaagacct gccagagcga tggatcttcg gcgccgagcc gccacaaggc gtagccagcc
     1141 gggcgatatt tatcagaggc gcgcaagtgg ttgtaggctg tcaccgcgtc gaggaaccaa
     1201 acgcggtgtg tgctgccatc ttccaaataa gagaagcggg ggtttaacgt atcgggatcg
     1261 aggtcgatcg acaccttcga atcacgcgcg gcgagaatcg cctcttggaa ggtcaattct
     1321 tccgcatcat gacctccaac gatccagtcg taaccatagt tcgcgatcgc cacaatcgta
     1381 tgggcgggat cgagatctcg catccgcgcc cccagccggg tcgagaacca cgattgtgaa
     1441 gcgacgggcc ccggctgcga gtccgcccaa tgctcatcat atcccatcag gatcaggtaa
     1501 tcacacgcct tggcataggc cttgtaattc caatgcggat cgtcgaaggg cgccgcgacg
     1561 gcaataatca ggcccttcgc cttgaagatt gcatgggcct cactcagaaa agccagggtg
     1621 tccttgtgcg catcgtcggg gatctgctcg aaatcgatga cgaggccttg gagcttatgc
     1681 tcctcgatga acgccaggac gctgttcagg cgttcgcgcc ttctctccgg atcagccaga
     1741 aggcgcccga ggttggggcc atcccatttc gtgatcgacg agttctggat cattgccagg
     1801 atggctgctc caggcttctc cctccggatc atatcaagcg acttctgatc gagggtgacc
     1861 ttgaggtcca tcgagctccc ttgcagatag agccagctcg gaaccaccca atcgaggttt
     1921 tggatgttat ttttgagcga cgaaaagctg ctgtcgtccc agttcgcata gaaaccgact
     1981 gtcagcggct tctcgcggcg ctcgcctgga ttctttaagc cttttgcctt ggccagagcc
     2041 ccggccggag gctgcacgga gtgcggaaat tgtttcttcg tagcttgggc tttgcgcgca
     2101 agattacgaa ccgacggtag caaatgcgga actttcgcaa catcgttcag caggggataa
     2161 cgctggcttt gtgctgtctg caaagtttcg ggaaaggaac ggaagataaa gaggcttgaa
     2221 agaaaaatga ggcccgcgac gctggtcgcg atggctagcg cccaggctgc gcgcgagata
     2281 tgcccggcgc ggcgattatt cggatcgaag aaaataggtt tcgtcatggt tctcttaggg
     2341 tggggtcgcg cctgggtgat cggtccgagg ccgaggattg caatcggtgt tgggtgagac
     2401 gccgcgaatc aaatggttac gggagcaaaa tacgtgagca tggatgaacg caagctgtac
     2461 agcggcgaag ggatgctcca aacccggccg ccattatcga ttatcacgca attgtgtgac
     2521 acgtgttacg gtttgaggaa gctgttggcc tgccccgtca gataggaaaa gaccttcgcc
     2581 ctggtgttgg cgtccgaaat caccttgggc acaatcccgt aaagggcaat tttatcgcct
     2641 tctggacctt ggatgttgca gatgtatatg ccatcaggcg cattctgagc tggtttgata
     2701 cgtgatccct tcagcatgtc gtcggccgag ccttgggtcc acacctccag ggcaaagatt
     2761 tttcgagcgt gccttaccca tggcgggctg gaccacgggc ttttgtcccg gcgaaccgag
     2821 tgctggcaga aatagcttcc cgtgtcgttg acgcttcctg tgacgagaat cagagcttcc
     2881 tgcttcacgc tatactgtgg cgcgatcgat gcatcctccc gtatcagctt caaggcgata
     2941 tccaagaggt atcgctcgat gggctgaacc tggaaaatag aagctggccc cggcggctct
     3001 tgggagccct tgctcaaaat cttaagggat gcgccacggc tgccatcgcc ggcaagcgct
     3061 ccaccttgca cttctacctg aagcttgcct gtctcgggac tcacgccggg agcttgcgcg
     3121 cggctaagta cttttttctc ccaagccgcg gtggaaacca cgttcacgag tctcagggcg
     3181 tccgaccaaa atccgtcagc ggccgcggcc cccccaagag aaccggcgaa ggtgctgtcg
     3241 gtcgagaaca ccgcgatcag ggtctgacct gcgcctttca gcgctcggac gatctttccg
     3301 ctccgcagct cttccgccgt aacccacggc cgggcctcca gcacgcatcc agagccgacg
     3361 ctcgacagcc gggcgacatt tccaggctcc gccgtgctga tgagaagttc gttaggttcg
     3421 atgtctaccg gcaggctcag tgcgtagtgg ctgtcggttt cggcaaactc aaggccatga
     3481 agccgctctt tctctccaag ctttccccag ccctcggcga gccgccggtc cgcggagaca
     3541 tcgagtgcca gctcacagcc gcgaacaggc tcgcctgccg catcatggat ttcgatcttc
     3601 agatcctcgg cccgcacata aagaggctca acgacgagga caaggcgatc agtgtctttc
     3661 ctgactgtca cgagctggta ccgccgaagc agtttcttca gataggc
//
LOCUS       DATMGX010000984         2745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_86687, whole genome shotgun sequence.
ACCESSION   DATMGX010000984 DATMGX010000000
VERSION     DATMGX010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86687"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /locus_tag="VNR65_16135"
     CDS             complement(<1..1760)
                     /locus_tag="VNR65_16135"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWJ20246.1"
                     /translation="MNYFGLAPLTRDLQTSRRSFLIGATALGSSLVVGFSSEADVAPA
                     AKPGANPFVGYVQIAPDDTVTIYSSQMDMGQGIYHGIATLVQEELDADWTKVSVVGGF
                     GNTVLYGNVPFGGKIQMTGGSSGTASSWERYRRAGATARAMLIAAAAERWNVPIGEIK
                     AQHGTVSHASGRSATYGALAASAASGPVPMDVALKEPSEWKYIGNETLPRYDSAPKSN
                     GRQQYTIDLREPGMLTAVMIHPPLFGATLKSFDASKAKAMKGIVDVVSTPRGVAVVGT
                     GMWEVMQAREVVTVEWEDDKAEKRSSRDIAAEYRAAADKGGEVVAANIGDVDAAFAKA
                     AKVIEARYEFPYLAHAPLEPINAAVRMNADGTLEVWGGHQMPDVYQAVAAKVAGIAPD
                     KVILRVMKTGGGFGRRASVDSDIIVEAVATAKAVGWKAPVKVQWTREDDMRAGRYRPA
                     FTHKMRAALDDNGNLVALRDTLVGQSIAKDSFMEKGLVKNGVDVLSVEGMANQPYAIP
                     NLRVDLTTTTSPVSVLWWRSVGSTHTAYALETFIDEIAHAVGKDPVEFRLAMLKDKPR
                     HAAVLRLAAEKAGWSAPTPAG"
     gene            complement(1762..2217)
                     /locus_tag="VNR65_16140"
     CDS             complement(1762..2217)
                     /locus_tag="VNR65_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWJ20247.1"
                     /translation="MRITLNGQAIDLDVDPAKPLLWILRDDLGLVGTKYGCGQALCGA
                     CTVLVDGQPVRACQTGIADVVGRTVVTIEGVSGKVADVVQMTWTKLDVPQCGYCQSGQ
                     VVATIALLNANRKPTDNDINDALSGNICRCNTYHRIRAAVHEAAKLLEA"
BASE COUNT          564 a          863 c          752 g          566 t
ORIGIN      
        1 cccgctggcg ttggcgcgct ccatccggcc ttttcggcgg caagccgcag gacggcagcg
       61 tggcgcggct tgtccttcaa catcgcgagc cgaaactcca ccgggtcctt acccacagca
      121 tgcgcaatct cgtcgataaa ggtctcgagg gcatacgctg tgtgcgtgga tcccaccgaa
      181 cgccaccaga gcacagatac cggagaagtt gttgtagtta ggtcgacgcg caggtttgga
      241 atggcataag gctgattggc cattccctcc accgacagca cgtccactcc gttcttgacc
      301 aggcccttct ccatgaaact atctttggca atggactggc caacgagagt gtcgcgaaga
      361 gcgaccagat tgccgttgtc gtcgagcgcc gccctcatct tgtgggtaaa agcgggccgg
      421 tagcgaccgg cgcgcatatc atcctcgcgc gtccactgaa ccttgaccgg cgccttccat
      481 cccaccgcct tcgccgtggc caccgcctcg acaatgatgt ctgaatcgac ggacgcacgc
      541 cggccgaaac cgccccctgt cttcatcacg cgcaagatca ccttgtctgg cgcgatgccg
      601 gcaaccttgg ccgcgaccgc ctgataaacg tcaggcatct gatggccgcc ccatacctcc
      661 agagttccat cagcattcat tctgacggct gcgtttatcg gctccagggg tgcatgcgcc
      721 aggtatggga attcatagcg cgcttcgatc acttttgctg ccttggcaaa cgccgcgtcg
      781 acgtcaccga tattggccgc aacgacctcg cctcccttat cagcggcggc acggtattcg
      841 gcagcgatat ctcgggagct ccgcttttcg gctttatcat cctcccactc aacggtaacg
      901 acctcgcggg cttgcattac ctcccacatg cctgtcccaa cgacggcgac gcctcgtggc
      961 gtggagacga cgtcgacaat gcccttcatg gccttggcct tcgatgcgtc gaaggatttc
     1021 agggtcgccc cgaatagcgg cggatgaatc atgaccgccg tgagcatgcc gggctcacgc
     1081 aggtcgatcg tatactgctg gcggccgttc gattttgggg ctgaatcgta gcgcggcagc
     1141 gtctcgttgc cgatatattt ccactcgctc ggctccttca gcgcgacgtc catcggaacc
     1201 ggaccgctcg cggcgctggc ggcgagtgcg ccatatgtag ccgacctgcc agaagcgtga
     1261 ctcacagtgc catgctgggc cttgatctcg ccaattggga cattccaacg ttccgcggca
     1321 gccgcaatca gcatcgcgcg cgcggttgcg cctgcccgac ggtagcgctc ccaggacgaa
     1381 gccgtaccac tcgacccgcc cgtcatctga atctttccgc caaagggaac gttgccgtac
     1441 agcaccgtgt ttccgaaacc cccgacgacc gacaccttcg tccagtctgc atcgagttcc
     1501 tcctgaacga gcgtagcgat cccgtgatag ataccttggc ccatatccat ttgcgacgaa
     1561 tagatggtca cagtgtcatc tggcgcgatc tgcacgtagc cgacaaatgg atttgcgccc
     1621 ggcttagcgg ccggcgcgac atctgcttcc gaggagaaac cgacgacgag gctcgaaccg
     1681 agcgccgtcg ctccgatgag gaacgaacga cgcgatgtct gtaaatcccg agtgagaggg
     1741 gcaagaccaa agtaattcat ctcacgcctc caagagtttg gcggcttcgt ggaccgccgc
     1801 gcgaatacga tgataggtat tgcaacggca gatgttgccg cttagcgcat cgtttatgtc
     1861 gttgtcggtc ggctttcggt ttgcgttcaa tagggcaatc gttgccacga cctgaccaga
     1921 ctggcagtag ccgcactgcg gcacatcgag tttggtccag gtcatttgaa cgacgtccgc
     1981 gacctttcca gatacgccct cgatggttac gacagtgcga ccgacaacgt cggcgatacc
     2041 cgtctggcag gcgcgcaccg gctggccatc gacgagaacg gtgcatgctc cgcacagagc
     2101 ctggccacat ccgtacttgg taccgaccag gccgagatcg tccctcaaga tccagagcag
     2161 aggcttagcc ggatcaacgt caagatctat cgcctgacca ttcaaggtaa ttcgcatagc
     2221 ggctcctccc caatcaattt gacgaagact gcatggcact gggcgtacgc aatgttttga
     2281 aacggaagcc tagctagatc ggagggcgag ataagtctgc ggtcagatat accttcttga
     2341 ccgcgatcga caccaacgat ccggaatctc acctcgagaa tactctgccc cggattggag
     2401 cctccggcaa cctttatgaa tattcgaatg gcatccgtcg gacccgctag tgcactgcga
     2461 aatgtcaatg atcgctctag gcagggaaat tatcgaaaat tattcccgca tcgtatgggc
     2521 cgggcgcaga tgtctgacca tcactcgacg tattgcctcg tgccaactct acttccgaga
     2581 cttgcacgaa ccggaccacc agcgatgtcc gacttgagtc cgctttcgag gaccaaagcg
     2641 gaaaacatct gctcttagcg agtatttcgc cttttgaccc aactgcgaca ttgaggacgg
     2701 taacggaaat ggaccacgct cagcgcttgc gggaattgta gccct
//
LOCUS       DATMGX010000985         2983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_81495, whole genome shotgun sequence.
ACCESSION   DATMGX010000985 DATMGX010000000
VERSION     DATMGX010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81495"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VNR65_16145"
     CDS             <1..309
                     /locus_tag="VNR65_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20248.1"
                     /translation="EAEEALRGARTRMARKPPALARELLRNFPKRRARNARRRRKGGA
                     AKPGAPGAANGATTQAPLETSLSRGRPEDPRPDPQADLRHEVQDALEHRDLDHAGEQA
                     "
     gene            858..1616
                     /locus_tag="VNR65_16150"
     CDS             858..1616
                     /locus_tag="VNR65_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20249.1"
                     /translation="MRKTIGLTLVTMVALSAAAVAVANAVEGPFYKVGGSRLLENETR
                     LLLASAKPGTPFVLTGTGLTVTCTSLSLPVANEMQLIGSTGANAGKSLEALHFTGCTV
                     ASNGAGCDVENGLILTNLVSNLLGYSTPTKTGPILVLFVPDQAKDFVTIHFKALSGGE
                     CKVPSALVEGSVIGLARVNGANVVVGSEPAETLHGEVSFAGLNKTIWMESNGTLMSVK
                     SGFNVGGLGAKFTGIALLLVDSAAGPVNWGVFTA"
     gene            1885..>2983
                     /locus_tag="VNR65_16155"
     CDS             1885..>2983
                     /locus_tag="VNR65_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20250.1"
                     /translation="MTPEFKAGARVSPEAFSSDGSRVIGSSIAVFAGTEGYGEVAEAI
                     YESVRSGTDWVTSAISPPASLFPAQKLLDFNPDLDRGLWVAHRASQSINAEDLYIREP
                     DGAFVEVGPIVPSYATEGPAAGSSPLLNEYAVTVYAGASSDLSHVFFQIRGEGPLWTG
                     DTTAHNGQLSLYEYVGTGNAEPMLVGVNNEGHLISDCGTSLGLELPGIHNADTYNAIS
                     ADGETAFFTALNAGECGAEHAPAVNEVYARVGVGAGARTVAISEPSLADCEACQTSER
                     AAAEFAGASRDGSRVFFTTSQELLKGATGENLYEYDFHNRPGHKIIRISTGSATPEVQ
                     GVMRVSEDGSHVYFVAGGVLTSGPNGEGDEPV"
BASE COUNT          609 a          773 c          972 g          629 t
ORIGIN      
        1 gaggccgagg aggcgctgcg cggagcgcgc acgcggatgg ctcgcaagcc tccggcgctc
       61 gcgcgcgagc tgctgcgcaa cttccccaaa cggcgcgcgc gcaacgcgcg ccgccgccgc
      121 aaaggcggcg ccgccaagcc gggcgcgccc ggcgcggcga acggagccac gacgcaggcg
      181 ccgctcgaga cgtcgctctc gcgcgggcgc cccgaggacc cgaggcccga cccgcaggcg
      241 gatcttcgcc acgaagtcca agacgcgctc gagcaccgcg acctcgacca cgcgggggag
      301 caggcctagc ctctccccgg ccttctctcg ggaaatccca gcagatctgt ctctatctct
      361 gccttctcgg ctcatgctgg cggggaatag gctttgcccg catttcggtg ccattcgcag
      421 tctttggctc ggccccgttc taaaaacttc taaaaactcg agttgatctc cggccggtta
      481 tctggtaatg cttgttctgt cccgtatgtt gaaacttagt ttgtgagact ccgggacgat
      541 caacaacagg aaaagcggcc ctgagacgac gttagcaccg agcgtctcag agccgaatca
      601 tggtccgtcg tgtccagcga ccggaccgcg acaataggag catattgaga tggtcgcgac
      661 ctgtctagca ggcgtcacgc tcccgatcgc aaggccgttg gctcaaccgc gcttgattct
      721 gtcggcgggc gaatgccgcg ctccagaaac agccaactta tgcctttcgg acgggatcgg
      781 gggcactgac cgcgctatcg cgatcgcgcc atcgcaccgt gctccaaggt ccaaaggagc
      841 aaatagcgat gaatcgcatg agaaaaacaa tcgggctgac gctcgtaacc atggtcgcat
      901 tgagcgccgc ggctgtggca gtggccaatg cggtggaagg gccgttttac aaggttggag
      961 gaagtaggct gctagaaaac gaaacacgct tactgttagc gtcggcaaag ccaggcaccc
     1021 ccttcgtgct cactggcact gggctcacag tcacgtgcac aagtctgagc cttccagtgg
     1081 caaatgagat gcagctcata ggcagcactg gcgcgaacgc tggtaaaagc ttagaggctc
     1141 tccacttcac cggatgcacc gtcgccagca atggagcggg atgcgacgta gaaaatggct
     1201 taattctaac aaacttagtc tctaatttac tgggctactc aacgccgacc aagacagggc
     1261 caatcttagt gctgtttgtt ccggatcaag ccaaagactt cgtcacgatt cacttcaaag
     1321 cattatcggg tggagaatgc aaagtcccgt cagcgttagt ggaaggtagc gtgatcggat
     1381 tagcgagagt gaatggagcg aacgtagtgg ttggcagtga acctgcggaa acattgcacg
     1441 gcgaagtgag ctttgccggt ctcaataaga cgatttggat ggaaagcaac ggcacattga
     1501 tgtcagtaaa atctgggttc aacgtaggtg ggctcggagc gaaatttact ggcatcgcgt
     1561 tactgttagt cgattcagcg gctggccctg tgaactgggg tgtgtttacg gcatagcatc
     1621 cggcttcacc tgtgatttag tgtggtgcgg agtcgccggt gttggtggct ccgcactgtt
     1681 tgtccgttag gagcgttgag gaggagggtt gtggtgctcg caagcgtgac ggtgtcggta
     1741 actcgttgtc ggggtgtggc ggcttgtttg ctgtgtgcat tggctgtcgc gggtgtgacg
     1801 gcggctcctg ttgcggcgag ctgctcgaat agtggttttg tggggttcaa ccggttcctt
     1861 gcgggttgtg gagggtatga gagagtgacg ccagagttca aggcgggtgc tcgagtgtcg
     1921 cccgaagcat tttcgagtga tggctcgcgg gtaatcggtt cctcgattgc ggtgttcgcg
     1981 ggaaccgaag ggtatggtga ggtagcggaa gcgatctatg aatcggtgcg atctggcacc
     2041 gactgggtga cgtcagcgat cagtccgccg gcgtccttgt ttccagcaca aaagctcttg
     2101 gacttcaacc ccgatctgga tcgtggcttg tgggtcgcgc atcgagcttc gcagtccata
     2161 aacgctgaag acctatatat acgtgaaccg gatggggcgt ttgtggaggt cggaccgatc
     2221 gtgccgtcgt acgcgacaga aggccctgcg gccggctcat cgccgttgct caacgagtac
     2281 gctgtgactg tctatgcggg agcatcgagt gatctgtcgc atgtgttttt ccagattcga
     2341 ggtgaaggtc cgctgtggac gggggatacg acagctcaca acggccagct ttcattgtat
     2401 gagtatgtcg gtacggggaa cgccgaaccg atgctcgtag gtgtaaataa tgaaggccat
     2461 ctgatcagcg attgcggaac gtctttgggg ttggagttgc cgggaatcca caacgcggat
     2521 acgtataacg cgatatcagc tgacggagaa acagcgttct tcacggcgtt gaacgctggt
     2581 gaatgcgggg ccgaacatgc ccccgcagtc aatgaagtgt atgcgcgtgt tggggttggc
     2641 gctggggcgc ggaccgttgc gatctctgag ccgagcctgg cggattgcga agcgtgtcag
     2701 acgagcgaac gcgcggcggc ggaattcgca ggtgcctcac gggatgggtc aagagtgttc
     2761 ttcacgacgt cgcaggaact tttgaaagga gctacgggcg agaacttgta cgagtacgat
     2821 ttccataatc ggcctggtca taaaatcatt cggatctcga cgggctctgc gacgccggaa
     2881 gtacagggtg tgatgcgagt gtctgaggac ggttcacatg tgtactttgt agcggggggc
     2941 gtgttgacgt caggcccaaa cggggaaggc gatgaaccgg tca
//
LOCUS       DATMGX010000986         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_86743, whole genome shotgun sequence.
ACCESSION   DATMGX010000986 DATMGX010000000
VERSION     DATMGX010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86743"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VNR65_16160"
     CDS             complement(<1..312)
                     /locus_tag="VNR65_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20251.1"
                     /translation="MKSRSLQRKIAKELLVWAIAIGGAYYTFSSFEPLFDRAKQAASR
                     AGFAHLFEFETNSPRLARFNTKVLNSSSVPLRGTISANQEPTGARKVVLSANDYGHFH
                     AV"
     gene            complement(366..1070)
                     /locus_tag="VNR65_16165"
     CDS             complement(366..1070)
                     /locus_tag="VNR65_16165"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HWJ20252.1"
                     /translation="MIFLAIGLLAVSIVLYVLTGEGDTIAGLPRDDFPRVAGLLSLLI
                     FLMAGAARLTAGKAAEALKALGSWAAIGLVVVALYAYRSELGEVRDRVVQELQPGSVQ
                     TVSPGMSGHGAVVSIRKTGDGHFIARAQVNGKSVRMIVDTGASTVVLRPEDARKAGIQ
                     VTALDYSVPVETANGRAFAARVKLERVSLGELTLEKVEALVTRPGALQQSLLGMSFLS
                     RLRSYEFSGNKLLMRS"
     gene            1234..>2094
                     /gene="dnaG"
                     /locus_tag="VNR65_16170"
     CDS             1234..>2094
                     /gene="dnaG"
                     /locus_tag="VNR65_16170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWJ20253.1"
                     /translation="MRYPADLLDEIRSRVPVSRVVERHVKLRRAGREYVGLSPFKTEK
                     TPSFTVNDHKGFYHCFASGEHGDVFTFLMKVEGLAFPEAVERLAADAGVILPAPKPVD
                     EATASHETRVREALEEACHFFESALAGREGSTARAYLEKRGVQFSEIKLFRLGYAPDN
                     KAALKQHLGGKGFSLAEMQDAGLLIHGDDIAIPYDRFRGRLIFPIMDAKRRVIAFGGR
                     ALLPDQQPKYLNSPETELFHKGHVLFNLAAAREAARTAKTVIVAEGYMDVIALARAGF
                     TNSVAPLGTAL"
BASE COUNT          450 a          609 c          599 g          436 t
ORIGIN      
        1 gaccgcgtga aagtggccgt aatcattcgc gctgagcacg acctttcgcg ctccggttgg
       61 ttcctggttc gcagagatcg tcccgcgcag gggcaccgat gatgagttca gaacctttgt
      121 gttgaagcgc gccaatcttg gggaattcgt ttcaaactcg aaaagatgcg caaatccagc
      181 gcgcgaggct gcctgcttgg cgcggtcgaa aagaggctca aaggaagaga atgtgtagta
      241 tgcgccgcct atagcaatgg cccatacaag aagttctttt gcaattttac gttgaagtga
      301 ccggcttttc atagtcacgg ctctccgccg tctttgcccc tcgacggaaa aactggtgca
      361 ccaacctagc ttctcatgag gagcttgttc cctgagaatt catatgacct caaacggctt
      421 aaaaaactca taccgagcaa gctttgttga agtgcgcctg ggcgggtgac cagggcttcg
      481 accttttcca aggtcaattc tccgagagaa acgcgctcga gtttcacgcg ggccgcaaat
      541 gcccggccgt tcgcggtctc aacgggcacg ctatagtcca gcgctgtgac ctggattccg
      601 gctttgcgcg catcttccgg ccgaagcacc accgttgagg cgccggtgtc aacgatcatg
      661 cggacgctct tgccgttgac ctgcgccctg gcgatgaaat gcccatcgcc ggtcttgcgg
      721 atcgagacga ccgcgccgtg ccccgacatc cccggcgata ctgtctggac cgaccccggc
      781 tgcagttcct ggacgacgcg gtcgcgaact tcgccaagct cgcttcgata ggcatacagc
      841 gccacgacca cgagaccgat cgccgcccag cttccaagcg ccttcaaggc ctcggcggct
      901 tttcccgctg tgagccttgc tgcgccagcc atgagaaaaa taaggaggga gaggaggcct
      961 gctacgcgcg ggaaatcgtc ccgcggcaaa ccggctatcg tatcgccttc gccggtcagc
     1021 acatacaaca caatactaac ggcaagcagt ccaatcgcga ggaaaatcat agatcgtcca
     1081 ttttttgaac gcgggcgata tataggtgga tatcgggaaa aagacaggtt cctttatctg
     1141 agcatactaa gccttcagcg aatcgctcca taattcgcac attaaccttt ctctctatcg
     1201 ccgttgagaa cagaataggc cagagcagca cttatgcgat acccagccga tttgctcgac
     1261 gagatacgaa gccgcgtccc agtgagccgt gtggtggagc gtcatgtcaa gctaaggcgt
     1321 gcaggacgcg aatatgtcgg cttatcgccc ttcaagacgg aaaaaacccc ttcgttcacg
     1381 gttaacgacc ataagggctt ctaccactgc ttcgcgagtg gcgagcacgg cgatgttttt
     1441 acctttctca tgaaggtgga agggctggcg tttcctgaag ccgtggagcg gcttgccgcg
     1501 gacgcggggg ttatcttgcc cgctccgaaa cctgtcgatg aagccacggc gagccacgag
     1561 acgcgcgtcc gggaagcgct cgaagaggcg tgccactttt tcgagtcagc gctcgctggt
     1621 cgcgagggga gcactgcacg agcttatctc gaaaagcgcg gcgttcagtt ctcggagatc
     1681 aagcttttca ggctcggcta tgcgcccgac aataaagcgg cgttaaagca gcatcttggc
     1741 ggcaagggct tttcgcttgc cgagatgcag gacgccggtc ttttgatcca cggcgacgac
     1801 attgcgatcc cctatgaccg ctttcgcggg cggttgatct tcccgatcat ggatgcaaaa
     1861 cgacgcgtca tcgcgtttgg aggccgcgct cttctgccgg atcagcagcc gaaatacctc
     1921 aactcgcccg aaacagagct gtttcacaag gggcacgtcc tcttcaatct cgcagcggcg
     1981 cgcgaggcgg cgagaactgc gaaaacggtc atcgtcgcgg aaggctatat ggacgtgatt
     2041 gcgcttgcgc gagcgggctt cacaaactcg gtggctccgc tcgggaccgc gcta
//
LOCUS       DATMGX010000987         2440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_336465, whole genome shotgun sequence.
ACCESSION   DATMGX010000987 DATMGX010000000
VERSION     DATMGX010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336465"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VNR65_16175"
     CDS             <1..1018
                     /locus_tag="VNR65_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWJ20254.1"
                     /translation="YLYKAFMIRAIMDSFDDAVGRLRAAGEPTRLRLLRLLQNGEFNV
                     KDLTQLLGQSQPRVSRHIKLLAEAGLIERYQEGSWVFVRASSDPGVRSFIAAVLSMVA
                     SDDPQILRDAERADQIRAARSLLAQTYFDENAAEWDHIRSLHVPEPDVEAAILEALGP
                     GPYELLLDLGTGTGRILELAASRARRLVGIDTNREMLKCARVRLDDAGLVNCSVRLAD
                     IFNLPFQEGSAGAVIVHQVLHFLDNPKAALIEAIRVLQPGGRLVLVDFAPHNLEFLRQ
                     EHAHVRLGFGAGEIAAWLEECGLGSRSYRELQADHVNNNPLTVAIWAGEKVSAKMQKW
                     RTAS"
     gene            1015..1932
                     /gene="metF"
                     /locus_tag="VNR65_16180"
     CDS             1015..1932
                     /gene="metF"
                     /locus_tag="VNR65_16180"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HWJ20255.1"
                     /translation="MTEASTHYHAIHPAIAVSFEFFPPKTAAMEASLWNAIRKLEPLA
                     PDFVSVTYGAGGSTRERTHATISRILKETSLKPAAHLTCVAATKEEVDGIIRGYWEDG
                     VRHIVALRGDPPEGAGAAYVPHPGGYCNGADLTAGIKRIGDFEVSVSAYPEKHPESPT
                     IETDLDMLQAKVDAGATRAMTQFFFDNDCYWRYLERVRARGINIPVVPGLIPIHNFKQ
                     VASFASRCGASIPAEVAERFDGLAEDPETTQLVAATVAAEQVMDLVAGGVTEFHFYTL
                     KRATLVYAICHLLGIRHQEPYAAPQRATA"
     gene            1946..>2440
                     /locus_tag="VNR65_16185"
     CDS             1946..>2440
                     /locus_tag="VNR65_16185"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HWJ20256.1"
                     /translation="MNRNGRIAALHAAAKHRILILDGAMGTMIQRHKLKEADYRGERF
                     KDWPSDLKGNNDLLALTQPDIIRGIHEAYFEAGSDIIETNTFNAQTVSMADYGMEALA
                     FEINVAAAKLARAAADKYTALTPEKPRFVAGAIGPTNRTASLSPDVNNPGFRNISFDE
                     LVAAY"
BASE COUNT          494 a          774 c          705 g          467 t
ORIGIN      
        1 atatctttat aaggcattca tgataagggc aatcatggat agctttgacg acgccgtcgg
       61 acgcttgcgg gccgctggcg aaccgacgcg tcttcgcctt ctccgcctcc tccagaacgg
      121 cgagttcaac gtcaaggatc tcacccagct cctcggtcag agccagcccc gcgtgagccg
      181 ccacatcaag ctgttggccg aagccggttt gatcgagcgt tatcaggaag gaagctgggt
      241 tttcgttcgg gcgtcgtccg accccggcgt tcgcagcttt atcgcagccg tcctatcgat
      301 ggtggcctcc gacgatccgc aaatccttcg cgatgcggaa cgcgcagacc aaatccgggc
      361 cgctcgctcg ctcctcgctc agacctattt cgacgaaaac gccgccgagt gggaccacat
      421 ccgatccttg cacgttcccg agcctgatgt ggaagcggca attctggaag cgctcggtcc
      481 cggcccctac gagcttctct tagaccttgg caccgggacg ggacgcattc tcgaactcgc
      541 cgccagccgg gcgcgccgcc tcgtcggcat cgacacaaac cgcgagatgc tgaaatgcgc
      601 gcgcgtccgt ctcgacgatg cgggcctcgt caattgcagc gtgcgcctcg cagatatttt
      661 taatcttcct tttcaagagg gaagcgcagg agcggtcatt gtccaccaag tgctccactt
      721 cctcgacaac ccgaaggctg ctttaattga ggcgattcgc gtcttgcagc ctggtggccg
      781 cctcgtcttg gtcgactttg cgcctcacaa ccttgaattc ctgcggcagg agcatgcgca
      841 tgtccgcctc ggtttcggag cgggtgaaat tgctgcgtgg cttgaggaat gcgggctggg
      901 ctccaggtcc taccgggagc tgcaagctga tcatgttaac aacaacccgc tgaccgtcgc
      961 catctgggca ggcgagaaag tcagcgcgaa aatgcaaaaa tggaggaccg cttcgtgacg
     1021 gaggcaagca cgcactacca cgcgatacat ccagcgatcg cggtcagttt cgagttcttt
     1081 cctcccaaga cggccgcgat ggaggcgtcg ctgtggaatg cgatccgcaa gcttgagccg
     1141 cttgcaccgg attttgtctc ggtgacatac ggagcgggcg gttccacacg cgaaaggacg
     1201 catgcgacga tatcccgtat cctgaaggaa acctcgctga aacccgctgc gcacttgacc
     1261 tgcgtcgcgg cgacgaaaga ggaggtcgac ggcatcattc gcggttactg ggaggacggc
     1321 gttcgccata tcgtcgccct ccgcggcgat ccgccggagg gcgcaggcgc ggcttacgtc
     1381 ccgcatcccg gcggctactg taacggcgcc gacctcaccg ccgggatcaa gcgcatcggg
     1441 gacttcgagg tttcagtctc agcctatccc gagaagcatc ccgaatcgcc gacgatcgag
     1501 accgacctcg acatgctgca ggcgaaggtc gatgcaggcg cgacgcgcgc catgacccaa
     1561 ttcttcttcg acaatgactg ttactggcgc tatctcgaac gggtccgcgc gcgcggcatc
     1621 aacattcccg tagtaccggg gctaatccca atccacaatt tcaagcaggt ggcgagcttc
     1681 gcctcccgct gcggagcctc gataccagcc gaggtagccg agcgcttcga cggcttggcg
     1741 gaagacccgg aaacgacgca gcttgttgcg gcaactgtgg cggccgagca ggtcatggac
     1801 cttgtcgcgg gcggcgtcac ggagtttcat ttctacactt tgaaacgtgc cactctcgtt
     1861 tacgccatct gccatctgct cgggatcagg catcaagaac cgtacgccgc gcctcagcgc
     1921 gccacggctt aaggatcaaa tccgcatgaa ccgcaacgga cgcattgccg ccctccacgc
     1981 cgccgcgaaa caccgcattc ttattctcga tggcgcgatg ggaaccatga tccagcgcca
     2041 caagcttaag gaggccgatt atcgtggcga gcgcttcaag gattggccga gcgatctcaa
     2101 aggcaacaac gaccttctcg cgctgaccca gcccgacatc attcgcggaa ttcacgaagc
     2161 ttattttgaa gccgggtcgg acatcatcga aacgaacacc ttcaatgcac agaccgtgtc
     2221 aatggcggac tacggtatgg aggcgctcgc tttcgaaatc aatgtcgcgg cggcgaaact
     2281 tgcccgagcg gcggcggaca aatacacagc actgacgccg gagaagccgc gcttcgtggc
     2341 tggcgccatc gggccgacga accgcacggc gtccctctcg ccagacgtaa acaatcccgg
     2401 atttcgtaac atctccttcg atgagctggt cgcggcctat
//
LOCUS       DATMGX010000988         2793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_54538, whole genome shotgun sequence.
ACCESSION   DATMGX010000988 DATMGX010000000
VERSION     DATMGX010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54538"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNR65_16190"
     CDS             <1..210
                     /locus_tag="VNR65_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20257.1"
                     /translation="EASEIPGDLAYLGSGFCLMVAVRIAWTNSSALLVILLVTLAGAV
                     HVFDMIRRFRAPDKAQKGRPSLERP"
     gene            complement(232..618)
                     /locus_tag="VNR65_16195"
     CDS             complement(232..618)
                     /locus_tag="VNR65_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HWJ20258.1"
                     /translation="MKTYIKIGVLSAALVWSVSAYAEGDAAHGEQVFKQCKVCHAIGP
                     AAKAGVGPAQNGVYGATAGTRAGYNYSTAMKEAGAKGLVWDDANLDKYLENPKAIVPG
                     TKMVFPGLKNEKDRQDVIAYLKANPA"
     gene            895..1815
                     /locus_tag="VNR65_16200"
     CDS             895..1815
                     /locus_tag="VNR65_16200"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWJ20259.1"
                     /translation="MSWRPSEEPVPGDRRSCDALNVVIVPRVRDLGGFNVRRALPSAQ
                     RQMVGPFIFWDQMGPAEFPPGEGIDVRPHPHIGLATLTYLFEGKIFHRDSLGSAQAIT
                     PGDVNWMTAGKGIAHSERTAPEWRAEPDRKMFGIQSWVALPEAAEEVKPDFFHHRAET
                     LPVIEAEGKRVRLIAGSLFGETSPVRTFSEMFYADVALSAGAKIPLQASHEERAIYVA
                     EGTIDISCDTFESGRLLVFRPGDEITVTAVAPSRLLFLGGEPMEGPRHIWWNFVSSSR
                     ERIEQAKEDWAQKRFDIVPGDEQDFIPLPP"
     gene            complement(1884..2621)
                     /gene="truA"
                     /locus_tag="VNR65_16205"
     CDS             complement(1884..2621)
                     /gene="truA"
                     /locus_tag="VNR65_16205"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWJ20260.1"
                     /translation="MPRYKVTIEYDGTPFSGWQIQANGPSVQGELARAIEGLTGQHII
                     PRGAGRTDAGVHARGQVAHFDLVRDYGPGTVRDALNFHFRPSPIAILDCEIVDESFDA
                     RFSAQARHYLYRICNRRTPLVLDRNRAWQVAPPLNVTAMQEAAQFLLGRHDFTTFRAA
                     QCQAKSPLRTLSSLDVVREGETIVITTSARSFLHHQVRSIAGSLKLVGEGKWQPADLR
                     RALEERNRMACGPVAPACGLYFMEVVY"
BASE COUNT          539 a          821 c          845 g          588 t
ORIGIN      
        1 gaggcgagcg agattccagg cgacctggcc taccttggct caggcttctg tctcatggtt
       61 gctgtgagga ttgcctggac gaattcgtcc gcccttctcg tgatccttct cgtcactttg
      121 gcgggagcgg tgcatgtctt cgatatgatc aggcgttttc gtgcgccgga caaagcgcaa
      181 aaagggcgcc ccagtctgga gcgcccgtaa cggcatcgga gccaaaaacc attaggcggg
      241 gttcgccttc agataggcga taacgtcctg acggtccttt tcgttcttca ggccagggaa
      301 gaccatcttg gtgcctggaa cgatagcctt cgggttttcg agatatttgt cgagattggc
      361 atcatcccaa acgaggccct tcgcgccagc ctctttcatc gccgtagagt aattgtagcc
      421 agcgcgtgtg cccgccgtcg cgccatacac gccgttttgc gccggcccga cgcccgcttt
      481 cgcggcgggc ccgatagcgt ggcagacctt gcattgcttg aaaacctgct caccatgcgc
      541 cgcatctccc tcggcatatg cggatacgga ccaaaccaag gccgcactga gcacgccgat
      601 cttgatgtat gttttcatat agctctccct caccctttcg actgttacct atcttgaagc
      661 aggcgattgc aaaccgaata tgttcacaaa cttaatgaca ctcaacgccg tctccagaga
      721 aaagtgtctt aaagtgtcta gcctcggctc ctactttcgg atgacccggc tttgcgggag
      781 ggttgctatg ccggtggagg gcggctcccg agaaacgacg aagattccaa agagaactcg
      841 tggtaatctt tgcgcaagtt tggcggcgtg gccgccctcg tatggagcgc acgcatgagt
      901 tggcggccga gcgaagagcc tgtccctggt gatcgcaggt cgtgcgatgc gcttaatgtc
      961 gtgatcgtac cgcgcgtgcg cgatctggga gggttcaacg ttcgccgcgc gttgccatcg
     1021 gcccagcggc agatggtagg cccattcatc ttctgggatc agatgggccc cgcggaattt
     1081 cccccaggcg agggaatcga tgtgcggccc catccgcata tcggccttgc aacgctgacc
     1141 tatctgtttg aggggaaaat ttttcatcgc gacagccttg gctcggcgca ggcgatcacg
     1201 ccaggcgatg tgaattggat gacggccggg aagggcatcg ctcactccga gagaacggct
     1261 cccgaatgga gagccgagcc ggatcgcaag atgttcggca tccagagctg ggtcgctttg
     1321 cccgaagccg cggaggaggt gaaaccggat ttcttccatc accgcgcgga aacattgcca
     1381 gttatcgaag ccgagggaaa gcgcgtgcgc ttgatcgcgg gctcgctctt tggtgaaacc
     1441 tcgcccgtac gcaccttttc ggagatgttt tacgctgacg ttgcgctatc tgctggcgca
     1501 aagatccccc tgcaagcctc ccatgaggag cgcgccatct acgtcgctga aggaacgatc
     1561 gacatcagct gcgacacctt cgaaagcggc cgcctcctcg ttttccgccc gggggatgag
     1621 atcacggtaa ccgctgtcgc gccaagccgc ctcttgtttc tcgggggcga acccatggag
     1681 gggccgcgcc acatctggtg gaattttgtg tcctcgtccc gcgagcgaat cgagcaggcg
     1741 aaagaagact gggcgcaaaa gcggttcgat atcgtgcccg gcgacgagca ggacttcatc
     1801 ccgctgcccc cgtaaatctc acctgggcgc tatcgtctct gctgccgctg tgtaacgcaa
     1861 acgagtctgc ggcttcgacc gcatcagtag acgacttcca tgaaatagag accgcaggcc
     1921 ggggccacag gcccgcaagc catgcgattg cgctcttcca gagcgcgccg caaatccgcg
     1981 ggctgccact ttccttcacc caccagcttc aaactaccgg caatcgagcg gacctgatga
     2041 tgaaggaaag agcgtgcgct tgttgtgatg acaatggttt cgccttctcg cacaacgtcc
     2101 aggctgctaa gcgtgcgcag cggtgatttt gcctggcatt gggccgcgcg gaaggttgtg
     2161 aaatcgtggc ggcctagaag gaattgggcg gcctcctgca tcgccgtgac atttagagga
     2221 ggggcaacct gccaagcacg gttgcggtcc aggacgagcg gcgtacggcg attgcagatg
     2281 cgatagaggt agtggcgggc ttgagctgag aagcgagcgt caaagctctc atctacgatt
     2341 tcgcagtcaa ggatcgcaat cggggaggga cgaaaatgaa aattcagagc gtctcgtacg
     2401 gttcccggcc catagtcgcg cacgaggtcg aaatgcgcga cctgaccgcg agcatgcacg
     2461 cccgcgtcgg ttcggcccgc gccacgtggg ataatgtgct gtccggttaa accctcgatg
     2521 gcccgcgcga gttctccctg aactgacggg ccattcgcct ggatctgcca gccagagaag
     2581 ggtgtgccgt catattcgat cgtgactttg tagcggggca ttcacaacga actcacggtg
     2641 catggctcgg gcagacccat cttacatccg gtcgcgttct tgtgtcggat cccgttcttg
     2701 tgtcgggccg cgctcttgtg ttgggcggcg ctcttgcgtc gggccgccgc gtggcccaac
     2761 gccactgccg cgtactggag gcatcatcac gat
//
LOCUS       DATMGX010000989         2155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_434215, whole genome shotgun sequence.
ACCESSION   DATMGX010000989 DATMGX010000000
VERSION     DATMGX010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434215"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..940)
                     /locus_tag="VNR65_16210"
     CDS             complement(134..940)
                     /locus_tag="VNR65_16210"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HWJ20261.1"
                     /translation="MSLIELAIATIVVAAAISSVMAGAWYLQWRTGNSGWVDVTWSLG
                     VGGVAAIAAAWPLGYDWPHWRQLIVAALAAFWCLRLGAHIAGRTRIAIDDPRNRNLII
                     QWGSDAPRRMFWFLQSQAAVGIILALSIAVAAHNPNPNLRIQDLIGLAILVAAIVLEA
                     IADQQLRTFKSDPANRKAVCDVGLWRWSRHPNYFFEWSSWLAYPIIAIDFTGQNPFGW
                     LAIAAPVCMYWVLVHVSGIPPLEDHMLRSRGDAFRAYQKRTRAFFPLPLK"
     gene            complement(950..1600)
                     /locus_tag="VNR65_16215"
     CDS             complement(950..1600)
                     /locus_tag="VNR65_16215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HWJ20262.1"
                     /translation="MSIEHHPTDSLLAAYAAGTLDHGQHIAIATHLVSCPQCRAFMRS
                     MEQVGGAVLTSLPPAPMASGALAAVEARLNEPERPAAAEMTPTVPETEVPGLPKFVRR
                     YPFGNWKWVAPSVHLRRIMLPFASDTRVFLLKSGPGTRMLEHAHTGFEMTCVLSGAFS
                     QGGGHFGPGDFDLGDETVDHEPVVDSGQDCVCLVAMQGELRLNGLLGRIIQPFVRL"
     gene            complement(1597..>2155)
                     /locus_tag="VNR65_16220"
     CDS             complement(1597..>2155)
                     /locus_tag="VNR65_16220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ20263.1"
                     /translation="KDRMQWGRLIRAIATDQDRAAFATLFEYFAPRIKSFLQRSGASE
                     ASAEELAQETMLAVWRKAALFDPESAGAAAWIFTIARNLRIDAHRRHSRGGVIETSDA
                     EIEFQIDDSPLPDSRLASAQSEERVRSALSQLSPDQVRVIELSFFEEKAHADIAKILE
                     IPLGTVKSRLRLAMGRLRNLLSELS"
BASE COUNT          477 a          656 c          623 g          399 t
ORIGIN      
        1 caatgccgtt tcgaggcggt tcttgccgcg agcaaattga cgcaggctac gcaacggcct
       61 ttagatgtag cgcgtggtgt catgttatcc acttggtttc gccagagcga caggtcgccg
      121 gctgaagcga acattatttc aacggcaacg ggaaaaaggc gcgcgttcgc ttttgatagg
      181 cgcgaaatgc gtcgccgcgc gaacgcagca tatgatcttc cagcggcggg atgccggaca
      241 cgtgtaccag cacccaatac atgcaaactg gcgcggctat cgcgagccat ccaaaggggt
      301 tttggccggt gaaatcgatc gcaataatcg ggtaggcaag ccacgacgac cattcgaaga
      361 agtaattcgg atgccgggac catcgccaaa gaccgacatc gcaaacggcc tttcggttgg
      421 cagggtcgct cttgaacgtg cgcagctgtt gatcggcgat cgcttccaag acaatggcag
      481 ccacaaggat tgccaaaccg atcagatcct gtattcgcag gttaggattt ggattgtgcg
      541 ccgcgaccgc gatcgagagc gcaagtataa tacccaccgc ggcttgcgac tgcaggaacc
      601 agaacattcg gcgcggagcg tcactacccc attgaatgat caaattgcga ttgcgtggat
      661 catcgatagc aatgcgggtg cgaccagcaa tgtgcgcgcc cagccgcaag caccagaatg
      721 cggcgagcgc tgccacgatc agctgccgcc aatgcggcca gtcgtatccg aggggccagg
      781 ctgcggcgat cgccgcgacc ccgccgacgc ccagcgacca ggttacgtcc acccaaccag
      841 aatttccggt tctccactgt aggtaccaag cccccgccat gaccgaacta atcgcggcgg
      901 cgacaactat tgtagcaatc gcgagctcga tgaggctcac cgattcaact cacaaacgga
      961 cgaacggctg aataatccga ccgagcagac cattcaggcg aagttccccc tgcatcgcaa
     1021 ccagacaaac gcaatcctgg ccgctatcga ccactggctc gtgatccaca gtctcatctc
     1081 ccaggtcaaa atccccaggg ccaaaatgcc ctccgccttg gctgaacgct ccggagagaa
     1141 cacatgtcat ctcaaatccg gtatgggcgt gctcaagcat tcttgtccca gggcccgact
     1201 tgagcaagaa gacccgcgta tcgctggcaa agggcagcat gatgcgacgc aaatgcacgg
     1261 agggcgcgac ccatttccaa tttccgaacg ggtagcgacg cacgaatttt gggagtccag
     1321 gaacttcggt ctcaggcacc gtgggtgtca tctccgctgc ggcgggccgc tcgggttcat
     1381 tcaaccgcgc ctcaacggca gccagcgcgc cactcgccat cggggcgggt gggaggctcg
     1441 tcagcaccgc accgccgacc tgctccattg agcgcatgaa ggcgcggcat tgcggacagg
     1501 acacgagatg cgttgctatt gcaatgtgct gcccgtgatc gagagtgccg gccgcgtagg
     1561 ccgccaacag cgaatcggtg gggtggtgtt caatgctcat gacagttcac tcagcagatt
     1621 tcgaagccgg cccatggcca agcgcagcct tgatttgact gttcctaggg gaatttccag
     1681 aattttcgcg atgtccgcgt gtgccttctc ctcgaagaag gacaactcga tgacccgcac
     1741 ctggtcagga gacagctgcg aaagtgcgga ccgaacccgc tcttcggact gcgccgaagc
     1801 cagtcgggag tcgggcagtg gcgagtcatc gatttgaaac tcgatctcgg catccgaggt
     1861 ctcgatcacg ccgccgcggc tatgccgccg gtgggcgtcg atgcgcagat ttcgggcaat
     1921 cgtgaaaatc caggccgctg caccggcgct ctccgggtcg aacaaagcgg ccttccgcca
     1981 gactgccagc atcgtctcct gagccagttc ctccgcgctc gcctcgctgg cacctgagcg
     2041 ttgcaaaaac gatttaatcc gtggggcaaa atactcaaac agggtagcaa atgcggctcg
     2101 gtcctgatct gtcgcgatcg ctcggatcag gcggccccat tgcattcgat cttta
//
LOCUS       DATMGX010000990         4050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_86926, whole genome shotgun sequence.
ACCESSION   DATMGX010000990 DATMGX010000000
VERSION     DATMGX010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86926"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VNR65_16225"
     CDS             complement(<1..354)
                     /locus_tag="VNR65_16225"
                     /inference="COORDINATES: protein motif:HMM:NF012458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20264.1"
                     /translation="MKAITAIAANKLGKFIAKDFRRIFGPAHDDMAERLGSLARSTIE
                     CLARSDALYHNYEHALQVTMVGRDILEGMTLSRRIEPTDYGHLIVACLFHDIGYMRGV
                     LSGDTEIEFVVDGSGK"
     gene            401..919
                     /locus_tag="VNR65_16230"
     CDS             401..919
                     /locus_tag="VNR65_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20265.1"
                     /translation="MKGETEGAGRLVPYAIKSGRRQSATRTRIPPIEFDLGAAAPDVG
                     DKMERSGRGPAALYFCDSVRDPTDGVELPFRIADVQASTWQDPLESSPTQWNQIRFIR
                     LDKFLDRLLWADGSTQATPYHLVIVGLAVITLASYRESWTRGPCSIDEKNPAAPRRGE
                     VWECDARLQTRR"
     gene            complement(1151..1431)
                     /locus_tag="VNR65_16235"
                     /pseudo
     CDS             complement(1151..1431)
                     /locus_tag="VNR65_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631264.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1887..2930
                     /locus_tag="VNR65_16240"
     CDS             1887..2930
                     /locus_tag="VNR65_16240"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWJ20266.1"
                     /translation="MERRTFLAGTAGLAVAAAGLGSKPAAADTLPLGDLPNWRYPDPR
                     VEALDKSFKYKVGNAAIERIATGFRWAEGPVYIRDYRRLIWSDIPNNRMMSWNEEDGH
                     VSVFRQPSNYANGNTRDRQGRLLSCEHDARRVSRTEHDGTIITIIDSFDGKKLNAPND
                     VVVAADDSIWFTDPGYGIFGNYEGHLDKSEQPPRVYRVDGKTGKATIVIDSFDKPNGL
                     AFSPDEKKLYIIDSGLTHGGRSNMRVFDVDVSTGKVTNDKVFAENFAPGFTDGVRTDI
                     DGNVWCSMGWADPKEDGVRCYAPSGDLIGKIHLPETCANLCFGGPKRNRLFMCGSTSV
                     YATYVETQGALLP"
     gene            3164..>4050
                     /locus_tag="VNR65_16245"
     CDS             3164..>4050
                     /locus_tag="VNR65_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWJ20267.1"
                     /translation="MTTTATFIAAMAIVGATHAPALAQDANALENAGKNGKDWMSYHG
                     SYQSWHYSPLDQINTNNIKKLKIAFIHQVGHSTRGVQSMPLAKDGILYYSASYSKVFA
                     LKGDTGEVLWSFVPKLDDDLVARQTHSPYNRGMAMSEGKLYIGTVDGRLFALDMKTGK
                     PVWETKLINSQKLTVGFTGAPLVVKDKVIIGAQGGEWPGRGPIFGVDAKTGQKVWEFL
                     TVAGTPDAEKTWGNESWRTGGGGGWMPGTYDAETNSVWWGTANPAPLYDWSGPDYKTG
                     GARPGDNLYTTSVIALDPDT"
BASE COUNT          909 a         1196 c         1178 g          767 t
ORIGIN      
        1 ctttccgctc ccgtccacca cgaattcaat ttccgtgtcg ccgctcagaa cgccgcgcat
       61 gtagccgata tcatggaaca gacaggcgac aatcaaatga ccgtaatccg taggttctat
      121 tcttcgtgaa agtgtcattc cttcaagaat gtcccgcccg accattgtga cttgtaacgc
      181 gtgctcgtag ttgtggtaga gagcgtcgct tctggcgaga cattcaatcg tactacgcgc
      241 caaggagcct aaacgctccg ccatatcgtc gtgtgctgga ccaaaaatac ggcgaaaatc
      301 tttggcgata aacttgccca acttattggc cgctattgcc gtgattgcct tcaagcgccc
      361 atcccccaag acggcaaaat gctatcatgt cgcgcaaaaa gtgaaaggcg aaacggaagg
      421 agcgggacga ctagtgccct atgcgatcaa aagcggacgt cgacaatccg cgacaagaac
      481 ccgcattccg cccattgaat tcgatcttgg cgctgcggct cctgacgtcg gcgacaagat
      541 ggagcgcagc ggtcgcggcc ccgctgcgct ctatttttgc gatagtgtcc gcgacccgac
      601 agacggcgtt gaacttccgt tccgtatcgc cgatgttcag gcttccacat ggcaggatcc
      661 tctcgaatca agccccacgc aatggaatca aatccgattc atccgactcg acaagttttt
      721 ggatcggctc ttatgggctg atggctctac gcaagccacg ccatatcacc tggtaatcgt
      781 cggcttagct gtgatcacgc tcgcctcgta tcgagaaagc tggacacgcg gcccatgcag
      841 cattgacgaa aaaaaccccg ccgctcccag acggggtgaa gtttgggagt gcgacgcgag
      901 attacaaacg cgccgctaga tagattatgt cacgaattcg gcccgtaagc tgcacatcag
      961 cgatgcccac aagactgttg cgatggcggc atagaagtca gtgggcctgt cgtttttgct
     1021 gtggtgacaa tgtcatcggt agcgcgcgag cttgcggcgt cccccatcta acgaactggg
     1081 acaaggagcg gggtcaggac aaggccaacc cggaccactc ggcgatcatg gggccgatcg
     1141 cgcataacct gagacccaaa cgctcttcgg tccgttcgat aatggtcctt gctgcaccga
     1201 cctcggccta gcccgggagg ccccgacgtc aacgatgacg ccgaacttca tgtcgatgaa
     1261 atagatgtcg gagtaagcga agattgctgg tcctttgtga gccccggtcc actgggccgc
     1321 cggatgggat ggcgagacga actttgggac gacgtcgctg gcggcgcccc acgctgtatc
     1381 gtcgagggtc gccaaacact cctccatagc gcgacttgac cttgcaggat cgcgatccct
     1441 gcgccaaacg cccacccgcg atcgggcgtt gcttattcgc gcggtcgggt cgcccgtgcg
     1501 ctcgatctag ctgcccggtc tcgatgatcc acggctagtg gctcttgcgt gaggatcgca
     1561 agcgtagcct accgcgccta gcgtcatcga tcgtgggcgc catggctttt atgtgtccga
     1621 aaaaattgaa gtgaccgcaa ctgcgaacag aatggaatca ttgtcatcgc agcgtcatcg
     1681 cagcttttta tggaatgagc actcttgacc agtgttccgt tgcgggcgca gtgtgtcgtc
     1741 tgttcattcg atgacggatc cgtccgcgag tccgcgatac aattaaaaaa ataacgtgga
     1801 ggaacacgca cgacacttaa tggaagcgcc ttcacgagcg ccgccgacat tccccaaaca
     1861 attccatcta aagggaggaa agtctcatgg aaagacgaac gtttctggcg ggcactgccg
     1921 gcctggccgt cgccgcagca gggttgggga gcaaacccgc cgcagcagac acgctgccgc
     1981 tcggcgatct tcctaattgg cgttaccctg atcctcgcgt cgaggcgctc gacaaaagct
     2041 ttaaatacaa agtcggcaat gccgccatcg agcgcattgc caccgggttt cgatgggccg
     2101 aagggccggt ctacattcgg gactatcgcc gtttaatctg gagtgacatt ccgaataatc
     2161 gaatgatgag ctggaacgag gaagacggcc acgtaagcgt gtttcgccag ccatcgaact
     2221 atgcgaacgg caatacccga gaccgccagg gccgattgct cagttgcgag cacgacgccc
     2281 ggcgggtctc gcgcaccgag cacgacggca ccatcattac gatcatcgat agctttgacg
     2341 gcaagaaact caatgcgccg aacgatgtcg tggtggccgc tgacgactcg atctggttca
     2401 ccgaccccgg ctacggcatt ttcgggaatt acgagggtca cctcgacaag tccgagcagc
     2461 cgccccgggt ttatagagtc gatggcaaga ccggtaaggc gacgatcgtc atcgatagct
     2521 tcgacaagcc gaacggcctt gctttttctc cggacgagaa gaagctgtac atcatcgact
     2581 caggcttgac ccatggtggt cgttctaaca tgcgggtgtt cgacgttgac gtcagtaccg
     2641 gcaaggtaac caacgacaag gtgtttgccg agaattttgc gcccggcttc actgacggcg
     2701 tgcgcaccga tatcgacggc aatgtgtggt gcagcatggg gtgggccgat cccaaggagg
     2761 acggggtgcg ttgctacgct ccgagcggcg atctgattgg taagatccac cttcctgaga
     2821 cctgcgctaa tctgtgcttc ggcggcccga agcgaaaccg cctgtttatg tgcggaagca
     2881 catcggtcta cgccacctac gtcgagaccc aaggcgcgct gttgccatga ccgcggcgaa
     2941 gacgccagcg actatggggc aatgaacctg acgaatgaca gggaatggcc acgatcggac
     3001 gcgcaccaag tggccgtcaa gacaagaacg cgcggtttga gtaatagcca gacggggcca
     3061 tcggggcgca agacgaaacg ctccgatacc ttgtccaacc agcaagcaat gccggaaacc
     3121 ggcaatcagg gaggtctgca aagtgactgt caggaaaagc ttgatgacga ccaccgcgac
     3181 gttcatcgcg gccatggcaa tcgtgggggc tacgcacgct ccggcattgg cacaggatgc
     3241 caatgcactc gaaaacgccg gcaagaacgg caaggattgg atgagctacc acggcagcta
     3301 ccagtcctgg cactacagcc cgctcgatca gatcaatacc aacaacatca agaaactgaa
     3361 gatcgccttc atccaccagg tcggtcactc gacgcgcggc gtgcagtcga tgccgctggc
     3421 gaaggacggc atcctctatt actcggcgtc ctacagcaaa gtgttcgcgc tcaagggcga
     3481 caccggcgaa gtgctgtggt cgttcgttcc caagctcgat gacgacctgg tcgcccgcca
     3541 gacccactcg ccctacaatc gcggaatggc tatgagcgaa ggcaagctct acatcggcac
     3601 cgtcgatggt cggcttttcg cactcgacat gaagacagga aagccggtct gggagaccaa
     3661 gctgatcaac tcccagaagc tcaccgtcgg ctttaccggg gcgccgctcg tggtcaagga
     3721 caaggtaatc atcggcgctc agggcggcga gtggccgggc cgcgggccga ttttcggcgt
     3781 cgatgccaag accggtcaga aggtctggga attcctcacc gtcgctggca ccccggacgc
     3841 cgagaaaacc tggggcaacg agtcctggcg caccggcggt ggcggtggct ggatgcctgg
     3901 cacctacgac gccgagacca attcggtgtg gtggggcacg gccaacccgg cgcccctgta
     3961 cgactggagc ggaccggatt acaagaccgg cggcgcgcgg ccgggcgaca acctctacac
     4021 gacgtcggtg atcgcgctcg accccgacac
//
LOCUS       DATMGX010000991         2562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_341776, whole genome shotgun sequence.
ACCESSION   DATMGX010000991 DATMGX010000000
VERSION     DATMGX010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341776"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VNR65_16250"
     CDS             complement(<1..404)
                     /locus_tag="VNR65_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HWJ20268.1"
                     /translation="MSLETPDKIRSLQRKLYHKAKTEPEFRFYLLYDKICREDILRHA
                     YSLARDNAGAPGVDGVTFRQIEAFGMEAWLAGLREELVSKTYRADPVRRVAIPKPGGG
                     ERPLGIPTIRDRVAQTAAKIVLEPIFEAGFED"
     gene            complement(909..2060)
                     /locus_tag="VNR65_16255"
     CDS             complement(909..2060)
                     /locus_tag="VNR65_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041204923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HWJ20269.1"
                     /translation="MNHSVKRVEFVRKYLDGIFAEDVHAKRVMSLANGAVGVMTGASL
                     AVSIIGQSLAQARGLLSKHAIKQVDRLLSNEGIVVWDMFAPWVAEVVGARKTIVVAMD
                     WTDFDADDQATLALSLVTNHGRATPLLWLTVLKDELKDQRNDFEDLCLARLAKVLPEG
                     VAVTILADRGFGDTKLFDFLDTLGFAYVIRFRGNIHVSAADGETRPAAAWVGKGGRAR
                     KLRNAEVTAGRHKVGAVVCVRARDMKEAWCLATNNAEATAREIMNYYAKRWTIEPGFR
                     DTKDLRFGMGLSVLRIADPQRRDRLLLLNAFAIVLLTLLGAAGESLGMDRHLKVNTAK
                     HRTHSLFRQGCMLYELIPNMPDARLRPLVARFHEYLNQHEAINQTFATV"
     gene            2122..>2562
                     /locus_tag="VNR65_16260"
     CDS             2122..>2562
                     /locus_tag="VNR65_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20270.1"
                     /translation="MSPRENEGIPEPWEWSDAELADAIIKIYNVTKKDKIAESALENI
                     ARNIKNWRKGQSACRHEWWGSPLLIFFGREPEHDLKKFRDEFRRSLIEAWNERSHVNE
                     LEKYGLQITEDADIDIIYDFDSESYHEKDRAHDDISKEQFWQWHR"
BASE COUNT          547 a          797 c          692 g          526 t
ORIGIN      
        1 ctatcctcga aacccgcctc gaagatcggc tccaacacaa tcttggcggc ggtctgcgcg
       61 acccgatccc tgatcgtcgg gatgccgagc gggcgctcgc cgccccccgg cttcgggatc
      121 gccacccgcc gcaccggatc ggcccgatac gtcttcgaga cgagttcctc gcgcaagccc
      181 gcgagccagg cttccatgcc aaacgcctcg atttgcctga acgtcactcc gtccacgccc
      241 ggtgcgcccg cattgtcgcg ggcgagactg taggcgtggc gcagaatatc ttcgcgacaa
      301 atcttgtcgt agagcaagta gaagcggaac tcgggctccg tcttcgcctt gtggtagagc
      361 tttctctgca ggctcctgat cttatctggc gtttcgaggc tcatcgccaa tcaccatctc
      421 ctcacccgct tcgaaagcat gacagaagtc agggcccttc cctcccccgg cattacccgg
      481 gttcaacggt aatactgccc tgtccgactc ccgcccggtc catcgccttg ccgcgatgtc
      541 gaagccgcga cctccgacca agacgggtct cccccgatta cccgcttcac ccttccagcg
      601 tgccgcccat taccccggcg gatcggatgg ctgcatgcgt cgattgcttc cccatccgtg
      661 cggccttccc cgagatgtcg ccgggtcggc atccgcatca ttactttcga ggcctgctca
      721 ggcttcacac acgttgcggc ccgctggatc gctcgaccgc ccaaggcggc cttcgtcacg
      781 aggcttcgat ccgtccgatt gctcgtccaa accgctcgtc agctaccagg acaatcgaca
      841 attccctggg tggaaccttc ctccactgtt gaaacgcgcc ttcggggcgc acgccatccc
      901 ctcatttttt acacagtcgc gaaggtttga ttaatcgctt cgtgttggtt cagatattcg
      961 tgaaatcgcg caacaagagg ccgcaaccga gcgtccggca tgtttgggat cagctcataa
     1021 agcatgcatc cttgccggaa caatgaatgg gtccgatgtt tggcggtatt gaccttgaga
     1081 tggcggtcca tgccgaggct ctcgccggcc gcgccaagaa gcgtcaataa aacgatggcg
     1141 aaggcgttca gaagaagcag cctgtcgcgc cgttgtggat cggcgatacg cagaacgctc
     1201 agacccatac cgaagcgaag gtccttcgta tcgcgaaacc ccggctcgat tgtccaacgt
     1261 ttggcgtaat agttcatgat ctcgcgcgct gtcgcctcgg cgttgttcgt ggccagacac
     1321 caggcctctt tcatatccct ggccctgacg cagacgaccg ctccaacctt gtgccggccc
     1381 gccgtgacct cggcgttgcg cagcttgcgc gcccgtcctc ccttgccgac ccacgccgcg
     1441 gcgggacgtg tctcgccatc ggccgcgctg acatggatat tgccgcgaaa tcggatgaca
     1501 taggcgaagc ccaacgtgtc gagaaagtcg aacagtttgg tgtcgccgaa cccgcgatcg
     1561 gccagaatcg tgaccgcgac gccctcgggc agcactttcg ccaaacgggc aaggcagagg
     1621 tcctcgaagt cgttgcgctg atctttcaac tcgtccttca acacggtcag ccacaacaag
     1681 ggcgtcgccc gcccgtggtt ggtgacaagg ctcaaggcca gcgtcgcctg atcatccgcg
     1741 tcgaaatccg tccaatccat ggccacgaca atcgtcttgc gtgcgcccac aacttccgcc
     1801 acccacgggg cgaacatgtc ccacacgacg atgccttcat tgctcagcag ccggtcgacc
     1861 tgcttgatcg cgtgcttact cagaaggccg cgcgcctggg ccagggactg gccgatgatc
     1921 gacaccgcca gcgacgcacc cgtcatgacg ccgacggcgc cattggcgag cgacatcacg
     1981 cgcttggcgt gaacatcttc ggcgaagatg ccgtcgaggt atttgcgaac aaactcaaca
     2041 cgcttgacag aatggttcat ggcctcgctt cgctcagacg ggattgtcca atcccgtcat
     2101 tcgctatgaa aattggccaa tatgagtccc cgggaaaatg aggggattcc tgagccttgg
     2161 gagtggtctg atgctgaatt ggcggacgcc atcattaaaa tatacaacgt cacaaaaaag
     2221 gacaaaatag ccgaaagcgc attagaaaac attgccagaa atataaaaaa ctggaggaaa
     2281 gggcagtctg cgtgtcgtca tgaatggtgg ggatctccgc ttttgatatt ctttggaagg
     2341 gagccagaac atgacttgaa aaagtttcgt gatgaatttc gaaggtcgct tattgaggcg
     2401 tggaacgaac gtagtcacgt taatgaactc gaaaaatacg gcctgcaaat aaccgaagat
     2461 gcggacatag atattattta tgatttcgat tctgaatctt accatgaaaa agatagggcg
     2521 catgacgaca tatcgaaaga gcagttctgg caatggcaca ga
//
LOCUS       DATMGX010000992         2709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_244991, whole genome shotgun sequence.
ACCESSION   DATMGX010000992 DATMGX010000000
VERSION     DATMGX010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244991"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VNR65_16265"
     CDS             complement(<1..587)
                     /locus_tag="VNR65_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20271.1"
                     /translation="MTLSLVALLLSPKIQFPGGAQTALIVLRWIHFVAGITWVGLLYF
                     FNLVNVPFLQELDAKSRGTVVPKLMPRALWWFRWSSVVTVLAGIAYWMHIVATDARNA
                     AALGEPASAGMMFGSFFGLWTLAFAIEMGALMSPAEALRKGPVLGAIVAIAVVAASYI
                     FLGLNQHGWESNPALAIGIGGGLGCFMMLNVWGVV"
     gene            852..1154
                     /gene="hfq"
                     /locus_tag="VNR65_16270"
     CDS             852..1154
                     /gene="hfq"
                     /locus_tag="VNR65_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWJ20272.1"
                     /translation="MENKAAQNIQDGFLNTARKDKVVVTIYLISGIKLIGRIRSFDKY
                     SVVLDTNNQEELIFKHAISTVVMSRSHHSERHFEHRAIAEPAAPAEAETANTASEG"
     gene            1461..2636
                     /gene="hflX"
                     /locus_tag="VNR65_16275"
     CDS             1461..2636
                     /gene="hflX"
                     /locus_tag="VNR65_16275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWJ20273.1"
                     /translation="MAELRELATSAGAAVIGEFLQRRQKPDAATLIGSGKVEELKGAV
                     ASSSPDLVIFDHELTPSQQRNLERELDLRVIDRTQLILDIFARHARTREGQIQVELAQ
                     LQYLLPRLAGRGVQMSQLGGGIGTRGPGETQLETDRRKIYRHIRHVKEQLENVRRIRG
                     QQRQRRESVPIATVALVGYTNAGKSTLFNALTNAGVLESKRMFATLDPTLRSVTLPSR
                     RQVLLSDTVGFIRNLPHTLVSAFRATLEEVQRASLLLHVSDATSPTAAEQQAQVEEVL
                     RELESQDKPRLYVMNKVDLLPEKKRDSLRDTESVIHVSAAKGLGLDRLLERIDELIAE
                     DPVRRVRMRVPQSEGKALAMLDAKARIFAREYRDGNVELEVQAPESLLRRLKQFVAE"
BASE COUNT          600 a          812 c          813 g          484 t
ORIGIN      
        1 cacaccacgc cccaaacgtt gagcatcatg aagcagccca gtccgccgcc aatgccgatt
       61 gccagggctg gattgctctc ccagccatgc tgattcaggc ccagaaaaat gtacgacgcc
      121 gccaccaccg cgatcgcgac gatggcgccc agcacgggac ccttcctgag ggcctcggcc
      181 ggggacatca gcgctcccat ctcgatcgcg aacgccagcg tccacagtcc gaagaagctg
      241 ccgaacatca tcccagcgga cgccggctcg ccgagggccg ccgcattgcg agcgtcggta
      301 gccacgatgt gcatccagta ggcgatgccg gccaggacgg tcaccaccga gctccagcga
      361 aaccaccaca aagctcgcgg catcagcttg gggacgaccg ttcctcggga cttcgcgtcg
      421 agttcctgta ggaagggcac gttcaccagg ttgaagaaat acaacagccc aacccaggtg
      481 atgccggcga caaaatggat ccaccgaagg acgattaggg cggtttgcgc ccccccaggg
      541 aattggatct tgggcgagag aagtagcgcc actagcgaga gagtcatctt gatcacctat
      601 gttcgagccg ggtatcctgc gcacgcttcc ggattaggag cagtgcgcga attctacatc
      661 acacggcctt gtgcttgttt tagttgaaat cgcgctgccg ccgccttatc gtaaaatggc
      721 ttatgattgc ttccgtggcc cttgtgggta taacgatgaa tcggctgcgg acgatttcta
      781 caggcccgga atcgtaacgg ggccgcgaat ctgggctgtg ctgttttttg catgttgcca
      841 gggaggaacg tatggaaaac aaggcagcac agaacataca agacggattt ctaaatactg
      901 cccgtaaaga taaggtcgta gttactattt acctgatcag tgggataaaa ctgataggaa
      961 gaattcgctc cttcgacaag tattctgtag ttctcgacac caacaaccag gaggagttga
     1021 tctttaagca cgccatttcc acggtggtca tgtcgcgttc ccatcattcc gaacgtcact
     1081 ttgagcaccg cgcgatagcg gaaccagccg ctccggcgga agccgagacc gcgaacacgg
     1141 cctccgaggg ctaatctatt tctcgcgcgg gtggaatgaa ctccagagcg aatgacccca
     1201 catggcaaac cggccggagc caaccagagc gagtcttcct ggtgggagtg gaattctcct
     1261 cccggtcagc agcgggccgc ggcgccgaac ctcgttcctc gaaggaccgg caagcgggac
     1321 ccgataacct agcggcttct cgttcccggc aacaatccac cgggaaagtt ccgcccagcg
     1381 cgcgcgtggc gcgcagcgcc gccgcctctc acctgcacgc ggaagatcgg gaacattgcg
     1441 gattttcggc cgaagagtcg atggccgaat tacgcgagtt ggccaccagc gcgggagcag
     1501 ccgtcatcgg cgaattcctg cagcggcgcc agaaaccgga tgccgctacg ctcatcggca
     1561 gcgggaaggt ggaggagctg aaaggcgcgg tcgcatcgag ttcgcccgat ctggtcatct
     1621 tcgatcacga gctgacgcca tcgcagcagc gcaacctgga gcgtgaactc gatctgcggg
     1681 tgatcgatcg cacccaactc atcctcgaca tcttcgcccg tcatgcgcgc actcgtgagg
     1741 gacagatcca ggtggaactg gcgcaactgc aatacctgct gccgcgactg gcgggacgcg
     1801 gcgtacagat gtcgcagctc ggcggcggca ttgggacccg cggtcccggt gaaacccagt
     1861 tggagacgga ccgccgcaaa atctaccggc acattcgcca cgtaaaagag cagctggaaa
     1921 atgttcgccg catccgggga cagcagcggc agcggcgtga atcggttccc atcgcgacgg
     1981 tggcgctggt cggctatacc aatgccggga aatcgactct attcaatgcg ctcacgaatg
     2041 ccggcgtgct ggagagcaag cgcatgttcg ccacgctcga tcccacgctg cgtagcgtga
     2101 ccctgccttc gcggcggcaa gtcctgttgt ctgacactgt gggattcatc cgaaacctgc
     2161 cacacacgct ggtttcggcg tttcgtgcca cgctggaaga ggtgcagcgc gcctcgctgc
     2221 tattgcatgt gtccgacgcc accagcccaa cggcggccga gcagcaggcg caggtcgaag
     2281 aagtgctgcg cgagttggaa tcccaggaca agccgcggct ctacgtgatg aacaaagtag
     2341 acctgctgcc agagaagaaa cgcgacagtc tgcgcgatac ggagagcgtt atccacgtct
     2401 cggccgcaaa agggcttggg ctggacaggt tattggaacg cattgacgaa ctcattgccg
     2461 aagatccggt gcgccgggtc cgtatgcgcg tgcctcagtc ggaaggcaaa gcgctggcca
     2521 tgctggatgc caaagcgcgg atatttgcgc gggagtatcg cgacggcaac gttgaactcg
     2581 aggtgcaagc ccccgaatcg ctgctcagaa ggctaaagca gttcgtggcg gaataagctt
     2641 tcacttctgg aagaacattt tcgacgcggc gagcgccata aagaccgcaa aggtcttacg
     2701 cagcactgg
//
LOCUS       DATMGX010000993         2855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_55041, whole genome shotgun sequence.
ACCESSION   DATMGX010000993 DATMGX010000000
VERSION     DATMGX010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55041"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..400
                     /locus_tag="VNR65_16280"
     CDS             161..400
                     /locus_tag="VNR65_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20274.1"
                     /translation="MLTLKRACKSRPSGEWSDDDYDVFDGEQHIGRVMWTHAAPADRR
                     WFWTITARMPQSTHDRGYAATREEAMAQFKAWWMR"
     gene            397..1056
                     /locus_tag="VNR65_16285"
     CDS             397..1056
                     /locus_tag="VNR65_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20275.1"
                     /translation="MTDIKANTWAICERVAEAQAMLHDHLEGGRFTADEIVRKLAALM
                     KEEGLLRAMWEGWLFSARHSTTDYNRGELAGVRVRAADGLHRLAARSPSATWPGRLDA
                     LQSHKRHNAGIPCESCALERIPEKKGNAVMRFVLCVLLVTLSPSFAFAAKGTMSLSQA
                     HAAAEKGDAKAAAAIATTIDRQTCLNGCANRGYKKGQCSDACRPGICDPGGEQPYCVA
                     K"
     gene            complement(1332..1754)
                     /locus_tag="VNR65_16290"
     CDS             complement(1332..1754)
                     /locus_tag="VNR65_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20276.1"
                     /translation="MHKAWAAIACALVVSGCAVMRAKEANQAQATMVGMTKEQVLACM
                     GVPAGKAGEGATEVWSYNSGNGQTVGFGSSSANVFGGHGYANGYGFSSMVTEQRFCTV
                     NVTMINGHVDRLNYVGPTGGLITQGEQCAFAIENCIQH"
     gene            complement(2143..2478)
                     /locus_tag="VNR65_16295"
     CDS             complement(2143..2478)
                     /locus_tag="VNR65_16295"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HWJ20277.1"
                     /translation="MHFCLTAQYTPQAINAMLDDDTSNRYEAVKKLVETGGGKLISMY
                     SYGAEGPSVMVIMDVPEPSMAAAITGVAVAGGAIHNVKVTRLMTQDEVANVRQKARQI
                     RSAYKAPGK"
BASE COUNT          598 a          827 c          861 g          569 t
ORIGIN      
        1 cgccggcgtg gcggatatag atgcgatggc ggctaggatg aaaaacgcta acgctgattt
       61 ctgcatggct taccctccgg tcggcgacct cgcgtagaat aacccccggc gttgccaatt
      121 agttcacact cacgccaaaa acaggcgctg gcaacctcca atgctcaccc tcaaacgcgc
      181 ctgcaaatcc cgaccgagcg gcgagtggtc ggatgacgac tacgacgtgt tcgacggcga
      241 gcagcacatc ggccgcgtaa tgtggacgca cgcggcgccg gcagatcgcc gctggttctg
      301 gacgatcacc gcgcggatgc ctcaaagcac gcatgaccgg ggctacgcgg caacgcgcga
      361 ggaagcaatg gcgcagttca aagcatggtg gatgcgatga cggatattaa ggcaaacaca
      421 tgggcaatct gcgagcgggt tgcggaggcg caagcaatgc tccacgatca cctcgaaggc
      481 ggacgcttca ctgccgacga gatagtgcgg aagttggccg cactgatgaa agaggaaggc
      541 ttgctacggg cgatgtggga gggttggcta ttttccgccc gacactccac caccgattac
      601 aatcgcgggg aattagccgg tgttagagtt cgggccgcgg atggtctgca ccggcttgcg
      661 gcacgatcac cttcggcgac ctggccgggc aggctcgacg cgctgcagtc gcacaaacgc
      721 cacaacgcag gtatcccttg cgagagttgt gcgttggaac gaatacctga gaagaaggga
      781 aatgccgtca tgcgctttgt gctgtgcgtt ctgctggtaa cgttgtcgcc gagttttgct
      841 tttgctgcga agggaaccat gagcctgtcg caagcccatg ctgccgccga gaaaggtgac
      901 gctaaggccg cggccgccat cgccacgacg atcgatcgcc agacctgttt gaacggatgc
      961 gccaaccggg gctacaaaaa ggggcaatgc tctgatgcct gccggccggg aatctgtgat
     1021 ccggggggcg agcagcctta ttgcgtagcg aagtaggcgc gccaggcggc gctggcagcg
     1081 ttcagggcag cgtgcgagcg agaataggcc gctgagcatt ggacccaaaa aaacggccgc
     1141 agcggaatgc ggccgtagga cttagagagg tcgcccccgg cgcctagggg gtactggggg
     1201 ggcggcagcc ggaagcgacc gaaggcttaa cgcacgtccc ctctatcggt tcccgcggcc
     1261 tcgctgtgag cgttgcgggc acctgggcgc cgatgctcgg ccgaattggg cagagagagc
     1321 gccggtcgcg gttagtgctg tatgcagttc tcgatggcga acgcacactg ctcgccctgg
     1381 gtaattagac cgcccgttgg gccaacgtag ttcaaccgat cgacgtgccc gttgatcatc
     1441 gtcacattca cggtacagaa cctttgctct gtaaccatgg aggagaatcc atagccgtta
     1501 gcgtagccat gacctccgaa tacgttggcg ctcgaactgc caaagcctac ggtttgaccg
     1561 ttccctgagt tgtacgacca aacctcggta gctccttcgc cggcctttcc cgccggaacg
     1621 cccatgcaag ccagaacttg ttctttggtc atgccaacca tagtcgcctg cgcttgattg
     1681 gcctccttcg ctcgcattac ggcgcaccca gagacgacca gcgcgcatgc gatcgcagcc
     1741 cacgctttat gcatgacccc tcctcacccc ggctgatcat cggacaaccg gggcgagcgc
     1801 gcaacctatg gttatcgcgc gggccgcgaa ccggctcggt ctagccgcgc cgcggcgtgc
     1861 atcattcaaa ttcgaggatt gcaatcgctg atccggtcgc gctcgcaatc cggttccatg
     1921 cggacggcgg actaccgttc tggccaacgc catcgaccgt gaatgaggca tacggcacta
     1981 agtcaaatgt cgccatgggg ggtaaagcgg acattgaacg gccttagcgg tttgtccgct
     2041 tacgacccaa ctcagacatt gcacggttct tgccagcgaa tccttcgatc caaaaggtct
     2101 tgcggaaagt cggtggcagc tatgttggct gccaccgatc cttcacttgc cgggcgcctt
     2161 gtaggcactt cgaatttgac gggccttttg tcggacgttg gccacttcgt cctgtgtcat
     2221 caggcgtgtg actttaacgt tatgaattgc gccgccggct acagcgactc cggtgattgc
     2281 ggcggccata gagggctcag gcacgtccat gatcaccatc acgctggggc cttcggcacc
     2341 gtagctatac atcgagatta atttgccgcc cccagtttca accagcttct taaccgcttc
     2401 atagcgatta gacgtatcgt cgtccaacat agcgttgatg gcttgcggtg tgtattgtgc
     2461 tgttaagcag aaatgcatgg cgattgttct cccgttggtg tgtcgttaaa tcaacaatgc
     2521 gtatgcccac ttccgccgtt tggaaatgga ttacccgtgc ccccaaaaaa tgagaaagtt
     2581 gcgaagcccc gccgtgggca agacgccacc tgtaacttgc aggacttcgg cacatcccgc
     2641 tggcggctct gtcgatcatc ctacgttgaa gaattgcgcg ttgaaagagg ggcatgtcct
     2701 ctgttgggga aagcgtggtc ctccccctga aaagtggtcc gacctgaact atgtcttttg
     2761 acatgaagga ggaccgagat gccgaagaag aagcacaaac ctgaggagat cgtcgcgaag
     2821 ctacgacaag ccgatgtctt gatatcgcag gggca
//
LOCUS       DATMGX010000994         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_210366, whole genome shotgun sequence.
ACCESSION   DATMGX010000994 DATMGX010000000
VERSION     DATMGX010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210366"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VNR65_16300"
     CDS             complement(<1..815)
                     /locus_tag="VNR65_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20278.1"
                     /translation="MQSVAKSSDKSNIYKLSDHANGEQEGSSDHSDDITDKPAVTGLG
                     NKEPLGFGNSKTLEVSDEKPTAETPADDALAWLKIIQNATADLTADLPVSKRDDFGHS
                     FAEGTSSFEHSPTSRNLGSVRRRECVNEENRHEGYENEHDLAQKLSYRDAEPWQVPSH
                     TLRAAAMPYMAATGALAFLAGSAAVYFLMGSSSADVKARAVAPAPETQIEAPLVHVDQ
                     PAPKKGSFQRAVASGVSSDSTSFSGSKAGDLGAKPVESGASVEHQPQKWSDTV"
     gene            977..1387
                     /locus_tag="VNR65_16305"
     CDS             977..1387
                     /locus_tag="VNR65_16305"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWJ20279.1"
                     /translation="MAEFAVEVREHILIAHSLPDPAFGRAEGMHGGTFLVDVTFFREK
                     LSDKNIVVNIGLAHEVLKAVLAPLNYRNLDEKPELAGQLTTTEFLCRYIFDQLAEVIA
                     DGMLAEDGKALSRLRVTLQESHVARASYEAALTK"
     gene            1384..>2038
                     /locus_tag="VNR65_16310"
     CDS             1384..>2038
                     /locus_tag="VNR65_16310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ20280.1"
                     /translation="MTHCVFAIPGDLATPTGGYVYARKILPLLAERLGIEICPLPAGF
                     PLASEAELHEAAASLAAFKEPSTVFFIDGLAYGAMPVSTLEALKGPIVALVHHPLGLE
                     EGLTSSEKAHLLKAEEEALALARHVVVPSRGTVRELKRLFSVPAGKITVAEPGILRGK
                     RASGASAGQPLHIVSVGSLTPRKGYGVLIDALNDVRDLPWRATIAGSLDRSPETAALV
                     "
BASE COUNT          421 a          592 c          559 g          466 t
ORIGIN      
        1 tcgacagtgt ctgaccactt ctgaggctga tgttcgacgg acgctccaga ttcgaccggc
       61 ttggcgccca ggtctcctgc tttcgagccc gagaacgatg tactatcgct tgaaacgccg
      121 gaagccaccg cccgctggaa actccctttc ttcggggcag gctgatctac gtgcacaagc
      181 ggagcctcaa tctgggtttc cggcgcaggt gctaccgccc tcgctttgac atccgcagat
      241 gacgacccca tcaagaaata aaccgcagcg ctgccggcta agaaggcgag tgcgcccgtt
      301 gccgccatat acggcatagc tgctgccctt aaagtatgtg atgggacctg ccacggttcg
      361 gcatccctgt atgaaagctt ctgggcaagg tcgtgttcgt tttcgtaacc ctcgtgccgg
      421 ttctcttcgt tgacgcattc cctgcgccga acggaaccga ggtttctcga ggttggagaa
      481 tgctcaaagg atgaggtccc ctccgcaaag gagtgaccga aatcatcacg ctttgacacg
      541 ggaagatccg cggttaaatc tgcggttgcg ttctggatga tcttgagcca tgcaagtgca
      601 tcgtctgcgg gcgtctccgc agtcggcttc tcatcagaca cctcaagcgt tttagaattg
      661 ccaaacccca ggggctcttt attgcccagc cccgttacag ccggcttgtc tgtgatatca
      721 tcggaatgat cggagctgcc ctcttgctca ccgttcgcat gatcactcaa cttgtagatg
      781 ttacttttgt cgctgctctt ggctacgctt tgcataaaga cgcccctttc ccaaaagtct
      841 tctgctcagc tatacaccat ttaaatgatt cgctgtcctt ggaagggaaa gctgatgctt
      901 tcacaggtgt taataacatg caatgttagt aggcgatctg attttgtgac taaaacccta
      961 gcaaaggaaa acccaattgg ctgaatttgc ggttgaagta cgcgaacata ttctgattgc
     1021 acacagtttg cccgatcctg cattcgggcg ggcggagggc atgcatggcg gaacctttct
     1081 cgtagacgta acattctttc gcgaaaagct gagcgacaag aacattgtcg tcaatatcgg
     1141 cctcgctcat gaagtcctga aagccgtgct tgcgccgctc aattaccgaa atctcgacga
     1201 gaaacccgaa ttggcagggc agcttacgac gacggaattt ttatgccgct atattttcga
     1261 ccagctggct gaggtcatcg ctgacggaat gctggccgaa gacggcaaag cgctctcacg
     1321 cctccgggtg acgcttcaag agagccacgt ggcccgcgcg tcgtatgagg ctgcactgac
     1381 aaaatgacgc actgcgtgtt cgcgatcccc ggtgatctcg caactcccac cggaggatat
     1441 gtctatgcgc gcaagatcct cccgctcctg gcggagaggc ttggcatcga aatttgcccg
     1501 cttccggccg gtttcccgct cgcgtctgaa gctgagttgc atgaggcggc ggccagtctg
     1561 gctgccttca aagagcccag cacggtcttt ttcatcgacg ggcttgctta tggcgcaatg
     1621 cccgtatcga cgcttgaggc tctaaaaggg ccgatcgtgg ctttggtcca tcatccactt
     1681 ggccttgaag agggacttac atcttcagaa aaagcccatt tactgaaagc ggaggaggag
     1741 gcgctggccc tggcccggca tgtcgtggtg ccaagccgtg ggacggtgcg ggaactcaaa
     1801 cggttattta gcgtccccgc cgggaaaatt acggtcgcgg agcccggcat ccttcgcggg
     1861 aaacgcgcct ctggcgcttc ggccgggcaa ccgcttcata tcgtgagtgt cggcagtctc
     1921 acaccccgca aaggctacgg cgtcctcatc gacgccctga acgatgtgcg cgatctgccc
     1981 tggagagcca cgatcgcagg ctccctggac cggtcgcccg aaaccgccgc ccttgtcc
//
LOCUS       DATMGX010000995         2961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_91306, whole genome shotgun sequence.
ACCESSION   DATMGX010000995 DATMGX010000000
VERSION     DATMGX010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91306"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VNR65_16315"
     CDS             complement(<1..1201)
                     /locus_tag="VNR65_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HWJ20281.1"
                     /translation="MDTDGLRCRFQRLEPFLDERGRRLFAANEALSLGRGGTSAVAAA
                     TGIARSTIIRGVAELKQAGNEVSSRIRRPGAGRRRAEATQPGLPEAIEALVDDAIRGD
                     PEATLRWVSRSQRNIVKTLRERGYKVSQKLVGRLLRQLGYSCQANRKTLEGSSHPDRD
                     AQFQYINGATKTAIANGEPVISVDTKKKELVGDFKNPGKELRRRGDPEPVRVHDFKLE
                     ELGKAAPYGVYDIAANQGWVNVGVDGDTAAFAVESIRRWWFRLGKSRYPEAKRLHITA
                     DSGGSNGARVRLWKVELQKLANETGLEITVTHLPPGTSKWNKIEHRLFAFISQNWRGK
                     PLVSHVVILQLIGATRTETGLTVTCDIDQNDYPRGIKVSDEEMAAINIERHAFHGEWN
                     YTIRPQPP"
     gene            complement(1412..2008)
                     /locus_tag="VNR65_16320"
     CDS             complement(1412..2008)
                     /locus_tag="VNR65_16320"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWJ20282.1"
                     /translation="MAQSQIHREVLEANRNYAENFGDKGNLALPPARGFAILTCMDAR
                     LDPAKYAGLSEGDAHVIRNAGGRASDDAIRSLVISHKLLGTREWFVIHHTDCGMELFD
                     DASIGSLLEENLETAVFDGQTWSNPKRGGGSPAGHYIRWLNFKDLAQSVVEDVKRIRE
                     HPLVPKHVPVYGYIYDVKTGRLNEVEAATAAGRATGQS"
     gene            2097..>2961
                     /locus_tag="VNR65_16325"
     CDS             2097..>2961
                     /locus_tag="VNR65_16325"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWJ20283.1"
                     /translation="MATIIASQAIITGAFSMTRQAIQLGWLPRLRVTQTSALGYGQIY
                     ISAVNWIMMVITIGLTIAFRKSDNLAAAYGIAVSGTMLMTSMLLYIAMVEKLNWGLPL
                     SMAVAGCFILVDSAFLIANSAKMLEGGYIPLALAAVIYGVMWVWHSGRDAVMQVLKSK
                     YQRTDTYLNDLAVRGIPRVPGTAVFLTRSKEGVPPVMAWHVRHNRALHERVMIINVSI
                     ESVPFIHLTDRFRFFEEAPNFWRAYVHYGYMERPAIPPILEEANAEGCDLDLSDVTYY
                     VGRETVVSRPDG"
BASE COUNT          535 a          891 c          871 g          664 t
ORIGIN      
        1 cgggcggttg tggcctgatg gtgtagttcc actcgccatg gaaagcgtgg cgttcgatgt
       61 tgattgcggc catctcttcg tcgctgactt tgataccccg tggatagtcg ttttgatcga
      121 tgtcgcaggt cacggtgagc ccggtttcag ttcttgttgc gccgatgagc tgcaagatga
      181 cgacatggct gacgagcggc ttgccgcgcc agttctgcga gatgaaggcg aagaggcgat
      241 gctctatttt gttccatttg cttgtgcccg gcggcagatg ggtgaccgta atttcaaggc
      301 cggtttcgtt ggccagcttc tgaagctcaa ccttccacag ccggacccgg gcgccattgc
      361 tgccgccgga atctgcggtg atgtggaggc gtttggcttc ggggtagcgt gacttgccaa
      421 gccggaacca ccagcggcgg atgctctcga cggcgaaagc ggccgtatcg ccgtcaacgc
      481 caacgttcac ccagccctgg ttggcggcga tgtcatagac gccatagggc gctgcctttc
      541 ccaattcttc gagcttgaag tcgtgaacgc gcaccggctc cgggtctccg cggcggcgca
      601 actccttgcc ggggttcttg aaatcgccca ccaattcctt cttcttcgtg tcaacggaga
      661 tcacaggctc gccattggcg atcgctgtct tggtcgcacc gttgatgtat tggaactgag
      721 catcgcgatc tggatggctc gatccctcga gcgtcttccg gttcgcttgg cagctatagc
      781 cgagctggcg cagcagtctg ccaacgagct tctggctgac cttatatccc cgttcgcgca
      841 aggtcttgac gatgttccgt tgactacggc tgacccagcg cagcgttgcc tccggatcgc
      901 cgcggatggc gtcgtcaacc agcgcctcga tggcctctgg cagaccgggt tgggttgcct
      961 ccgcacgccg gcgcccagcg cccggacgcc ggatgcgcga actgacctcg ttgccggctt
     1021 gcttcagttc ggcgacgcca cgaatgatcg tgctgcgcgc gatccccgtc gccgcggcaa
     1081 ccgcgcttgt tccgcctcgc cccagcgaca aggcttcgtt ggcggcgaac aaacgccggc
     1141 ccctctcgtc gagaaacggc tccagtctct gaaatcggca gcgcaagcca tcggtgtcca
     1201 tcgcgaaaca cgatagcctt attcgcggat tcttttcctg cgttcttcga ttcgtttatt
     1261 tcgcgtcagg cccttaggtc acgacggcgc ttaaacgatc gtaacctctc accggatgac
     1321 gtgcctggaa aagctacgat ttgaggataa tgcgccgccg ctcctggtag agactcggcg
     1381 cgagtctcta caaagggaag atggccgcct cctatgattg cccagtcgcg cggccggctg
     1441 ccgttgcagc ctcaacttcg ttcaagcggc cagtctttac gtcgtaaata taaccgtaga
     1501 ccggaacgtg cttcggaacg agaggatgct cgcgaatgcg tttcacgtcc tcgacgacgc
     1561 tttgggcgag atctttgaag ttgagccagc ggatatagtg gccagcgggc gatcctccgc
     1621 cacgcttggg attggaccaa gtctggccat cgaagaccgc ggtttcaaga ttttcctcga
     1681 gcaggctacc gatcgaggcg tcgtcgaaca actccatgcc gcaatcggtg tggtggatca
     1741 cgaaccattc ccgtgtgccg aggagcttgt gggaaatcac cagcgaacgg attgcatcgt
     1801 cactcgcgcg tccgccagcg ttgcggatga catgggcatc gccttccgaa aggccagcat
     1861 attttgcggg atcgagcctt gcatccatgc aggtcaggat cgcaaagcct cgggcgggcg
     1921 gcagggcaag gttgcccttg tctccgaaat tttccgcata gttccggttg gcctcgagca
     1981 cttcacgatg gatctggctt tgcgccatgg taacctcctt caggcggcgg tttagggaag
     2041 ctgtgagaga gcgcggctga cgctcatcgg gcgcgccctt cgtcatcctg gcgaccgtgg
     2101 ccacaatcat cgcgagccaa gccatcatca cgggcgcttt ctcgatgacg cggcaggcca
     2161 tccagctcgg ctggctgccc cgccttcggg tcacgcaaac ctccgcgctc ggctatggcc
     2221 aaatctatat cagcgccgtg aactggatca tgatggtcat caccatcggt ctcacgatcg
     2281 ctttccggaa atccgataat cttgccgccg cttatggcat cgctgtctcg ggcacaatgc
     2341 tgatgacctc gatgcttctc tacatcgcga tggttgaaaa actcaactgg ggcctgcctc
     2401 tgagcatggc cgtggccgga tgcttcatcc tggttgacag cgcgttcctg atcgcaaact
     2461 cggcaaagat gctcgaaggc gggtacatcc cgcttgccct tgccgctgtc atctatggcg
     2521 tgatgtgggt ctggcactcc gggcgcgatg ccgtgatgca agttctcaag tcgaaatatc
     2581 agcgaaccga tacatacctg aatgatctcg ccgtaagagg cattccccgc gtgcccggga
     2641 ctgcggtttt cctcacgcga tccaaggaag gtgtgccacc ggtcatggcc tggcacgtca
     2701 gacacaatcg tgcgctgcat gagcgggtca tgatcatcaa tgtatcgatc gaatccgtgc
     2761 ccttcattca cctgaccgat cgcttccgct tcttcgagga agccccgaat ttctggaggg
     2821 cttacgtgca ttacgggtac atggagcggc ctgcgatccc tcccatcctt gaagaagcga
     2881 atgcagaagg ctgcgatctc gatttgagcg acgtgaccta ctatgtaggc cgggagacgg
     2941 tcgtttcgcg tcctgacggc a
//
LOCUS       DATMGX010000996         2277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_203555, whole genome shotgun sequence.
ACCESSION   DATMGX010000996 DATMGX010000000
VERSION     DATMGX010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203555"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1130)
                     /locus_tag="VNR65_16330"
     CDS             complement(126..1130)
                     /locus_tag="VNR65_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWJ20284.1"
                     /translation="MTRVAIQSFDDPDRYFSAIRAMKGTGFVLPRRGAFEAKLTQLDF
                     GRVWLQSGSETLARSGHMSVDHERRPIGFLADESVQPVTLSGAELPVDHIAFFSPGAT
                     QYQRSFGSLRWSAMSLAPEDLEEASRRVADQVINVPSSTRYIGPPAAQLARLRRLHRE
                     AVSLAESSSPVLRNSVAAIHIESDLTHAMISCLTVGEEKRFHRAWYRHKHIMDRFEQW
                     VDAHPDRPAHLLEVCADIGISARSLTLCCQEQLGMSPVRYLWMRRMKLARRALQEAGP
                     STTVTVIALSYGFSHLGRFSVGYRSLFGELPSATLAQWPKIGECIPALRKTSQFARTG
                     "
     gene            1379..2053
                     /locus_tag="VNR65_16335"
     CDS             1379..2053
                     /locus_tag="VNR65_16335"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HWJ20285.1"
                     /translation="MNSYLARLRERWSDPLLTALTVLLVVMLFVVAPLQALGIFIFQA
                     FELVFAIVLVAGVFVMSGSRTAAVAMLISLVMASVGAISRLKAPSALDVNLFAGAWLI
                     MGVTLASVVAPAVFASGRVTYHHIMGAILLYLTIAVTFVALFTFVGSLVPKAFSGLSL
                     DDSPALASNLIYFSFVTLTTTGYGDIVPVHPVARSLCNLESIFGQLYPATLLARLVTL
                     ELADRT"
     gene            complement(2050..>2277)
                     /locus_tag="VNR65_16340"
     CDS             complement(2050..>2277)
                     /locus_tag="VNR65_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20286.1"
                     /translation="HHLKFARPLSLALKVSDEFTVPLCRDHHRNLHCHGNEIAWWANV
                     QIAPIELAKDLWQESLDRDLPISRQRPPSPD"
BASE COUNT          469 a          678 c          651 g          479 t
ORIGIN      
        1 cttgacctgg atcaagtgca gccttccgca ccccaggacg caaggaaggc gaacgaccgc
       61 ttcgaccgtt gccgccggca gcgacgcagg acgcgctgca caatcgccgc gaacgaaacc
      121 ctttgctatc cagttctggc aaactgggat gttttccgca gggccggtat acactcgcca
      181 atcttcggcc actgagcgag cgtcgcgctc ggcaattccc caaagagaga acgataaccg
      241 accgaaaagc gccccaggtg tgaaaagccg tagctcaaag cgatcaccgt cacggtcgtc
      301 gaaggacccg cctcctgcag cgccctgcgc gccagtttca tccgacgcat ccagagataa
      361 cgcaccgggc tcatgccgag ttgttcctgg cagcagagcg tcagggaccg cgccgatatg
      421 ccgatatcgg cgcagacttc caagaggtgc gcgggacgat cggggtgcgc gtcaacccac
      481 tgttcgaagc ggtccatgat atgcttgtgg cggtaccatg cacgatggaa acgcttttct
      541 tcacccaccg tcagacagga gatcatggcg tgggtgagat cgctttcgat gtgtattgcc
      601 gcaaccgaat tgcggaggac tgggctcgag gattcggcaa gcgaaacggc ctcgcgatgg
      661 aggcgcctca gccgtgctaa ctgcgccgcg ggaggcccga tataccgcgt cgatgacggg
      721 acattgataa cctggtccgc aacccgcctc gacgcttcct cgagatcttc cggcgcgagc
      781 gacattgccg accagcgaag ggaaccaaag ctgcgctgat attgcgtagc gccgggcgag
      841 aagaaagcga tgtgatcaac cggcagctct gcccctgaca gagtaaccgg ctgcacgctc
      901 tcgtcggcaa ggaagccgat cgggcggcgc tcgtgatcga cgctcatatg cccggaccgc
      961 gccagcgtct cgctgccaga ttgcagccaa acgcggccga agtccaattg cgtcagctta
     1021 gcctcgaaag caccgcgcct cggcaacaca aacccggtgc ccttcatggc gcgaatggcg
     1081 ctaaaatagc ggtcgggatc atcgaagctt tgtatcgcaa cgcgcgtcat accaaacagg
     1141 ccctcccttt cgcagatgat cgaaagtcca tccgaaccaa tcgaaactct atccgaacct
     1201 tggtgcttgt actattgaat tgcgacgaga tcgcaatagg gccggactcc aatccgggcg
     1261 acagcgtaaa gacttcaggc ggcagacagg cctgaaaacg acatcaaaga tcaaaaaacg
     1321 ccatcgctca cggtcatcgg ccggataact tccctttcag cacgaggtct ttaacgacat
     1381 gaacagctac ttagcgaggt tgcgggaacg gtggagtgat ccacttctga cggccctcac
     1441 ggtactactt gtggtcatgc tgtttgtcgt tgccccactc caggccctgg gaattttcat
     1501 ctttcaggcg ttcgagcttg tttttgcaat cgttcttgtt gccggcgttt tcgtcatgtc
     1561 ggggagcaga acggcggccg ttgcaatgct catctcactc gtcatggcga gcgtcggggc
     1621 catctctcgc ctgaaggcgc catctgctct cgatgtaaat ctcttcgcag gcgcctggtt
     1681 gatcatggga gttacgctgg cgagcgttgt cgcccccgcg gtattcgcct cgggacgcgt
     1741 tacctatcat catattatgg gcgccatctt gctttatctg acaatcgccg tcacctttgt
     1801 ggcgctattc acctttgtcg gatcgcttgt gcccaaggca ttctcgggcc tgtcgctgga
     1861 cgatagtccg gcgcttgcca gcaatctgat ctattttagt ttcgtgacgc tgaccacaac
     1921 gggctacggc gatatcgttc cggtgcatcc ggtagcgcgc agcctctgca atctggaatc
     1981 gatatttggc cagctctacc ccgcgacctt gcttgcgagg ttggtaacac tcgagcttgc
     2041 cgaccggact tagtccggac taggcggtcg ttggcgggaa attggcaggt cgcgatcgag
     2101 actttcttgc cagaggtcct ttgctaactc gatcggcgcg atctgaacgt tggcccacca
     2161 cgcgatctcg ttgccgtggc agtgcagatt tcgatgatga tcccggcaca agggtacggt
     2221 gaattcgtcg ctgaccttga gtgccagtga tagtggtcgc gcaaacttca gatggtg
//
LOCUS       DATMGX010000997         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_239095, whole genome shotgun sequence.
ACCESSION   DATMGX010000997 DATMGX010000000
VERSION     DATMGX010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239095"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            708..1010
                     /locus_tag="VNR65_16345"
     CDS             708..1010
                     /locus_tag="VNR65_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20287.1"
                     /translation="MAETRAGKMDPKLGSTLAYMAAALLRAYEADPRTPVNPPAMPNV
                     YRALRFRVHSTEITETIQQKELQAPPTEARPGLPAPTTEAEVSQAKQEDEVVILDY"
     gene            1602..>2030
                     /locus_tag="VNR65_16350"
     CDS             1602..>2030
                     /locus_tag="VNR65_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20288.1"
                     /translation="MAPSTTSKVTTDHDEIRRWAEARNAIPATVVRTKKDEHAEVVGL
                     DFPGFDGEGSLEEISWEQWFNKFDKLNLALLYQEDTAGGEKSNFNKIIRRETAQEVSS
                     AVGGRGRSATLRAPQRQASGGKRSAGTASKKSASGRKQAAG"
BASE COUNT          484 a          571 c          629 g          346 t
ORIGIN      
        1 agaagtcttc ttccgggaag tttcacttgg ccgtgtagtc ggctatgcgc ggaagtgcca
       61 gctggtgtca actcccgaga taccggcttg gaggagatgt ttcgcggcag gatttccgca
      121 cgcagcggaa cttcgagccg atgcgccggc agctcgaagg agggcggcag atccagctca
      181 ccgacgcccg agcgaagcca attgatgcgc cctagtacct tgtgcgcgat gcacgatgct
      241 tatgtgcggg gaatagccgg tttcgaagcc cggaccacgt aggcgaatcc gtccccgaaa
      301 gcgccgagcg agactgcacc taatctgcac ctgatgcttt atgatggtgt gctaagtgaa
      361 tgaaaacgca gcatattgtc gtctgtcgcc aaatagctct gacggtttat aaaaccgccg
      421 ccacaatcac catcaggtat gcccacttgg cagataatta gagaatgtcg aataaagcta
      481 agacaacagc agttggcaag tgtcgagcaa ggacgaaagc gggtcgccca tgtgccgctc
      541 cagcagtgca cggcggcgcc tattgcgcga tccacgcaga ccccaaccga gcagcccaac
      601 tcggtcgcaa ggggggtgct cgtaaccgta agaggtacga cggcgagatc caggaggttt
      661 cgattcctca atcggccgca gacgtgaggc gaatgctagc cgaggtcatg gcagaaaccc
      721 gcgcagggaa aatggacccg aagctgggta gcaccctcgc ctacatggcg gctgccctgt
      781 tgcgggcata tgaggcagat ccccggacgc cagttaaccc acccgcgatg cctaatgtct
      841 accgagcgct tcgtttcaga gtccacagta ccgaaattac cgagactatc cagcagaaag
      901 agcttcaagc gccacccacc gaggcacggc caggcctccc tgcgccaacg acagaagccg
      961 aggtctctca ggccaagcaa gaagatgagg tcgtgattct cgactactaa ccggacatcc
     1021 cttaactcac taagtgttag ccggagggcc gctcttcttt gccgcttagc cttctgataa
     1081 ttgccgcttt atgccgcttt aacggcatgg ggaagccaag catgttggtc aaggcgtccg
     1141 ccaggttgtg ccataatgtg ccgccgcaga gttgcttcga cggtgcgaca taccacgagg
     1201 agaaacgggc tcacagcgta ccgcgagaga aggaggcaac gacgagatag gtagcaatgt
     1261 caagaatccg acccggggat cgggggcgag cacactcggt cagaatcggc caaagctcgt
     1321 gtattcgata gtggcgatgt cgtctcggcg agctgcctca ggcgcgcgag ctcttcagag
     1381 tcgaaccaag tcaagagggg acggaggggt gtctgacaag accggatgga aaggccgtgg
     1441 tcaacagttg cgggactcgc gttggggcac accggaccct gcgccgcgcc cagggcccgg
     1501 ccaaaggaaa taattatgcg ctggaaccac gcaaagggta tacagtgtgg ggctgttggg
     1561 agctaaaacg gaaattccgg ctcaacaaat acggagcgca gatggcaccc agcaccactt
     1621 ccaaggtcac caccgatcac gacgagatcc gccgctgggc ggaagcgcgc aacgcgatac
     1681 ctgcgacggt cgttcgcacg aaaaaggacg aacacgcgga ggttgtcggc ctcgattttc
     1741 ccggcttcga cggtgagggc tcgctggagg agatcagctg ggagcaatgg ttcaacaagt
     1801 tcgacaagct caacctggcg ctcctctacc aggaagacac ggctggcgga gagaagagta
     1861 acttcaacaa gatcattcgc cgcgagactg cccaggaagt tagttccgca gtgggcggcc
     1921 gtggacgcag cgctactctg cgtgccccgc agcggcaagc ctcgggtggt aaaaggtccg
     1981 cgggcacggc gtcgaagaaa agcgcgagtg gacgtaagca ggcggccggg
//
LOCUS       DATMGX010000998         4142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_380202, whole genome shotgun sequence.
ACCESSION   DATMGX010000998 DATMGX010000000
VERSION     DATMGX010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380202"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VNR65_16355"
     CDS             complement(<1..407)
                     /locus_tag="VNR65_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20289.1"
                     /translation="MDPNFSKKNGDPVQVSNGLAQDLLTYSCGVPNAKTPKEVLDALH
                     AITSKRLHLNVYLAIRFPDRATDWDALELGKTVFLHESTPKGLWEEWIRQVPHKLPVG
                     YMLARMSLGPHTGTESLQMLEPIGVDRWGFELAL"
     gene            974..1120
                     /locus_tag="VNR65_16360"
     CDS             974..1120
                     /locus_tag="VNR65_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20290.1"
                     /translation="MKASEDLKSRLIASYESAVHNGLSPCNALAVMLEWVAEECARIR
                     ANRA"
     gene            complement(1169..2350)
                     /locus_tag="VNR65_16365"
     CDS             complement(1169..2350)
                     /locus_tag="VNR65_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20291.1"
                     /translation="MQGSKKLIPFTLKHILGSSLIVALAFVSIALYDGALQKNWAEEQ
                     IANESQAAAQAQTQRLSANLSRLWRTVEILADEASAHPANLQQSLIEARRFNPALTLL
                     GFAKVDGTVTAATDGEEISVAQAPWFASALKNPIVKAVTSETGDYSATIVLAKPLRNT
                     DGITVAVLFALAATRDFVTSAVGPAGWRSKSTLATNDGHQFMIWRNAERSFKRQQPSE
                     KASPPLSRSTDHNEWLTATAQVISDGAFPETGWSLTVARAKAEIDQRLAPVMLKLWAG
                     CFVIACLTMLAAAAWTAWIAAPLTTISRFAATAAEGNKVDPISETRFREAAILSSSLV
                     ELWTTLRRVNAPKPYRLVASEADVDGESDLIFDDLVLHINALMDQECLIHPGAKQING
                     Q"
     gene            complement(3296..3394)
                     /locus_tag="VNR65_16370"
     tRNA            complement(3296..3394)
                     /locus_tag="VNR65_16370"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(3482..>4142)
                     /locus_tag="VNR65_16375"
     CDS             complement(3482..>4142)
                     /locus_tag="VNR65_16375"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWJ20292.1"
                     /translation="GMACLVAGAIVLSWTGTPTSSGFIGPLAIVGACVAWGLDNNLTR
                     KVSLADPLQIVELKGLIAGPFNLALGLWAGASLPASSLLLIAGIVGFLGYGLSLVLFV
                     LALRNLGTARTGAYFSTAPFLGTAAAIMILHDPITAQLVAAGLLMALGVFLHLTENHD
                     HEHGHQPMEHAHPHTHDEHHRHAHGRGDPPGEPHTHRHHHKPMRHSHPHVPDMHHTHR
                     H"
BASE COUNT          914 a         1127 c         1196 g          905 t
ORIGIN      
        1 agagcgagtt caaagcccca ccggtccacg ccgataggtt cgagcatctg gaggctttca
       61 gttccggtgt gcgggccaag gctcatccgt gccagcatat agccgactgg gagtttgtga
      121 gggacttgcc tgatccattc ctcccacaag ccctttggcg tcgactcatg aagaaagact
      181 gtctttccaa gctctagagc gtcccaatcg gtggccctgt ccggaaagcg gatggcgagg
      241 tacacgttca gatggaggcg ctttgaggta atggcatgca aggcatcaag cacttctttc
      301 ggggtcttcg cgttcggcac accgcatgaa taagtgagga gatcctgggc gaggccattg
      361 gagacctgca caggatcacc gttcttttta ctaaaattcg ggtccatggc gcttatgcgt
      421 ttccgcaatt tgggtggcga gaccacatga aagtaaagtt cttaactgag cgaggcaatc
      481 tattcttaag ctgcaggcct atcccttaag cagtagcgct gtcccccgga acggggattg
      541 tggcaatggc caaattatgt agtttccagg cgcaaagcgc gtgaaagacg ctcagccgcg
      601 accccacaag ccttgagccg cagtggctgc cgcgccctcg cgtgctttgc agccggcttc
      661 tttccctaat aaagcaaatt tgacattcga gtctcgcttg ccacgagttc acgcttgaat
      721 gtcaaattca gaagtttcca tagaactaaa aagttgcagg tggtcggatg ctggaatgcg
      781 ctgcaagggg atgcgcaatg tcggaaccgg accactagac cagcttatct ttaatcgatg
      841 ttgatcttgg cggttggcaa aagagtctca tcgtttcttt tcgtgttgca gcatgaattt
      901 ccaggcgagg cctcggctta tgtccgatca agatcaagaa ggtttgtttc aacgaccccg
      961 caaggatcca agcgtgaagg caagcgagga cctcaagagc cgcctgattg ccagctacga
     1021 gagcgcggtc cacaatgggc tgtcgccttg caacgctctt gcggtgatgc tggagtgggt
     1081 tgcagaagaa tgtgcccgca tccgcgcgaa ccgggcatga agccccgtct ggaaaccaga
     1141 agtgcgactc aggctcgttg aaggctgcct attgtccgtt gatctgcttt gctccgggat
     1201 ggatcaggca ttcctgatcc atcagcgcat tgatgtggag cacgagatca tcgaagataa
     1261 gatcgctttc gccatcgacg tctgcctcgg aggcaaccag gcgatatggc tttggcgcat
     1321 tcactcttcg cagggtcgtc caaagctcta caagagagct actgaggatc gcggcctctc
     1381 tgaagcgggt ttcgctaatc ggatcgacct tgttgccttc cgcggcggtc gctgcaaacc
     1441 gggaaatcgt cgtcaagggc gcggcgatcc aagccgtcca tgccgcggcc gcaagcatcg
     1501 tcaggcatgc aatgacaaaa caaccggccc agagtttcag catcacaggc gcgagccgct
     1561 ggtcaatttc cgcctttgcc cgcgcaacgg tcaaagacca gccggtttcg ggaaaggccc
     1621 catcggaaat cacctgggca gtcgctgtaa gccattcgtt atgatcggtc gaccggctta
     1681 aagggggact tgctttttct gagggctgtt gccgcttgaa ggaacgctca gcgttccgcc
     1741 agatcataaa ctgatggcca tcattggtgg caagagtgct cttgcttcgc cagcccgccg
     1801 gacccacggc agacgtcaca aaatcgcgcg tggctgcgag cgcgaataga acagcaacgg
     1861 tgattccgtc ggtattcctc agtggcttgg ctagaacgat ggtggcagaa taatcccccg
     1921 tctcactcgt cactgctttc acaatcggat ttttcaaagc acttgcgaac cagggcgctt
     1981 gcgcaacgga gatctcttcc ccatcggtgg cagcggtaac ggtcccatcg actttggcga
     2041 agccaagcag ggtcaatgcc gggttgaaac ggcgtgcctc gataagcgac tgctgcaggt
     2101 ttgccggatg ggcggacgct tcatccgcaa gaatttcgac tgttcgccat agtcttgaaa
     2161 ggtttgcgct tagccgttgc gtctgagctt gcgccgccgc ttgcgactcg ttcgctatct
     2221 gctcttcggc ccagttcttc tgcaatgcgc catcgtacag tgcgatggac acaaacgcca
     2281 gagccacgat cagcgacgat cccaaaatgt gtttcaatgt gaacggtatg agcttcttgc
     2341 tgccttgcat ggacggagcg ccctttaatt cgaagtgctg cgctcattgg cgctgtcgcc
     2401 aggcaacctc gcgccatggc cactcagata cttcagccac atccgggacg gagccgaagg
     2461 gtgcgtgatc gagtggcgac ggtttgcatt cgccgttcac tgaggttatt ggcgcgaaca
     2521 atattattgt tgaggtcgac caaccgcgcg ctggcggctt ccagtgctga ttgagccgcg
     2581 tagtgagctg ttgaactcga ccctcgttcg cgagggtcag cgtcccttcg atcggtgctc
     2641 gaattgtagc taagggagca ttgatgtcag ccgtcacgct tgtttcagcg atatactcgc
     2701 tggcgatgat catggccgca acgccgacac gcgggacgga aagcagcctt gttctcgaca
     2761 tgacagctcg cacacgacta acaaacatcc acttctggtt gtatagaagc aaaagggaaa
     2821 gaaatgctga aatggagtcc tctcgtgaag ctcagacgaa acaggagcca cggatagagg
     2881 cgtgctcaga gccgggggtg gcggcgtacg cacacatgaa gagcttgctt cgagcggcga
     2941 tggctcggcc gagccccgct cccgcgtaaa cgtcatagcc gcgagacaaa acggttagcc
     3001 tttgcggcag acaagaaaaa gcgggccgca gcttgtctta agaaattcgc cggagctata
     3061 aataccttat catcgaagga ccatagcaat gcgtcgcgtc cctgaagaag caagcacttc
     3121 atcacatcgc gccaggggac cgcagccatc gaatacggtc tcatcgcggg attggttgtc
     3181 gtcactatct taaccacgct ggctgggccg aagttcaatg gcgtgttcaa cacggtggcg
     3241 accaatcttc aaatcgccgt gtcgggctga actcttctac tcatgaattt ggctttggtg
     3301 cgcgcggaga gtttcgaact cccgaccctc tgggtgtaaa gtagcaaaaa tcgtgttagc
     3361 tttttaaagt aaaaggaaaa ttctctacac gcgcttcgtg aattgccgtg agaaatcacg
     3421 gcgtgccatg ttcgtttgta aaacggcgtg gcacccatcg ttttaagttt tccctcgcgc
     3481 ctcaatgccg atgggtgtga tgcatgtccg gtacatgcgg gtggctgtgg cgcataggtt
     3541 tatggtgatg ccggtgtgtg tgcggttctc ctggcgggtc gcctcggcca tgagcatgcc
     3601 gatgatgctc atcatgagta tgggggtgtg catgctccat cggttgatga ccatgttcat
     3661 ggtcgtgatt ctctgtgagg tgcagaaaga cgccgagcgc catcagtaag ccggcggcaa
     3721 ccaactgagc cgtgatgggg tcatgcagga tcatgattgc agccgcggtc cccaggaacg
     3781 gagcggtcga gaaataagca cctgttctcg cggtccccaa atttcttaga gcgagaacga
     3841 aaagtacaag gctcaagcca tagccgagaa aaccaacgat ccctgcgatg aggagaagcg
     3901 agcttgccgg caggctcgct cctgcccaca agccaagcgc caggttgaat ggaccggcga
     3961 tcagaccttt caactcgacg atctggagcg ggtcggcaag cgacaccttg cgcgtgaggt
     4021 tgttgtcgag cccccaggcg acacaggccc cgactatggc gagcggccca atgaaccccg
     4081 acgaggtggg tgtcccagtc catgacagca cgatcgcccc tgccacgagg caagccatgc
     4141 cg
//
LOCUS       DATMGX010000999         4396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_342640, whole genome shotgun sequence.
ACCESSION   DATMGX010000999 DATMGX010000000
VERSION     DATMGX010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342640"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VNR65_16380"
     CDS             complement(<1..596)
                     /locus_tag="VNR65_16380"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWJ20293.1"
                     /translation="MATDQFAVSDQAASFDETRLKEQKEKVRRFRKRLQARDAFILPE
                     IWDVVSARVMADAGFDLIGISPDSLAWAQGLMPHERLPLDALVAAAEKITHNFAVPIN
                     ADLEGILGRSIEEIEIAVGAVVSAGCVGVVIGDGGRGGSHGIASMQDMTAAIRTAKAA
                     ALEMRVPAVITARTEAFLLDPPPHSPFETAVERAEAYFA"
     gene            complement(735..1562)
                     /locus_tag="VNR65_16385"
     CDS             complement(735..1562)
                     /locus_tag="VNR65_16385"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HWJ20294.1"
                     /translation="MTKLPRTLSLLSLFSLLAVLVLAAVTASAAKPKEQPFQTWKAFR
                     AAPLQSAPSSQSPVIASIPKNAVLTSFQPCTRGWCAVEYKGIRGWIYDIFIVEHSANP
                     VAHVPPGPPRLTKAALQRPSATPAPVAAASLRISYRVIGLGAEESLPIREAPLDTAPL
                     MGALSPAANAIAGLETCRRQWCLIEHDGVRGYVRSRFLARAGDAPSPKYGVDGESNMK
                     VFSYGAPDADVVGEIPFYAGGIVPVGDCNGEWCHVRYLGLVGFVDTRRLRPQTSPQG"
     gene            1727..2992
                     /locus_tag="VNR65_16390"
     CDS             1727..2992
                     /locus_tag="VNR65_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20295.1"
                     /translation="MAFQQACTRCALVFLILSLQAALLRAEEAQLKAAPELSASPSSS
                     APSPQARSAQPETSGLRSQALLASIDKILKRAATEREEAKTLPSRDKFLFDPVWTATR
                     EEREKEVRELLDGALSVVTDAPILKLQEDIHKQQDKIAANRDRIATLREKRLGVPQPG
                     LLPSIFTETTESIDKTIAALEEDIKRQEAGIVRIKQEIGTALAAAGVTLSQDQLDLLL
                     DGVLGGDLLKLMAAFEVAKIADERLATLVQQSDEDLKSARRYFAMHAALFAMLVQAQD
                     MLIERIDKTYLARLREITSSIDQTGAKTRELLRSAGREDQRKALDANLKAQDVSRRVS
                     VFYRDYLQAQRRLLAQARDRSVFDLRVADNTYETVEESFQLKALMDEARASFESVQKL
                     DHPGFEQIFRNENLRREFENLTQKLGPSS"
     gene            3121..4182
                     /locus_tag="VNR65_16395"
     CDS             3121..4182
                     /locus_tag="VNR65_16395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWJ20296.1"
                     /translation="MRIVLALLLLGAVSCEALGAEADCAYDPKLSHLSRIVAADSTHG
                     PIYGTALNDASNKAQSRTLNLRDHEIVLTFDDGPLGSNTQTVLDTLQKHCVKATFFSV
                     GRMAQQNPKLLQELDRRGHTIGAHTWSHPRAMHLLPADDAKLEIEKGFAAVSQALGKP
                     IAPFFRYPGLRDTPETAAYLASRNISIWSVDVVSGDTEPGATSARVASNVLSRVKQVG
                     KGIILFHDIKKPTAEALDGILTQLEKDGYKVVHVVSNTEYQPNPELIARADAFRTAPE
                     KATITGTWVASAKPEKEKAGKEDIKDGSVDVMHTEWIDLKHISDMLAGQASDVASARA
                     DQQGDGLTANSWTASGHVR"
BASE COUNT          962 a         1254 c         1312 g          868 t
ORIGIN      
        1 gcgaaatagg cctctgcgcg ttcgacggcc gtctcgaagg gcgagtgcgg cggcggatcg
       61 agaagaaacg cctcggttct tgccgtgata accgcaggaa ccctcatttc aagcgctgcc
      121 gcttttgcgg ttcggatcgc cgccgtcatg tcctgcatcg atgcaatgcc gtgtgacccg
      181 cctcgcccgc catcgccgat gacgactccg acgcagcccg ccgagaccac ggccccgacg
      241 gctatctcga tttcctctat tgagcgcccc aggatgccct caaggtctgc attgatcggc
      301 acggcaaaat tatgcgtgat cttttctgcg gcggctacga gtgcgtcgag cgggagccgc
      361 tcatggggca taaggccttg cgcccacgct aaggaatcgg gggaaattcc gatcaagtcg
      421 aatccagcgt ccgccatgac acgggccgat accacgtccc agatctcggg aagtataaag
      481 gcgtcgcgcg cttgcaaacg cttgcggaag cggcgcacct tttccttttg ttctttgagc
      541 ctggtctcgt cgaaactagc cgcctgatcc gaaacagcaa actggtctgt ggccataatg
      601 cacgacgact ctaacttgag gttgatagtc ggctttgact ggcgcaatca gccaatcctg
      661 tcttaactct ctcgttagaa tgctcccgtc gctgccgcaa gcaaaaagca ccgccatcca
      721 aaggcggctg cgcgctatcc ctgcggagag gtctgagggc gcaggcggcg ggtgtcgacg
      781 aagcccacaa gtccgagata acggacgtga caccactcgc cgttgcaatc gccaacaggt
      841 acaatccctc cagcatagaa tggaatctcg ccgacgacgt ccgcgtcggg ggccccataa
      901 ctgaagactt tcatattcga ctcgccgtcg acgccgtatt tcggagaagg cgcgtcccct
      961 gcgcgggcta gaaaccggct tcgaacatat ccgcgcacgc catcatgctc gatcaggcac
     1021 cattggcgcc tgcatgtctc aagtcctgcg atagcgttcg cggccggtga aagcgctccc
     1081 atcagaggag ctgtatcgag aggcgcttct cgaatgggaa ggctttcttc ggccccgagt
     1141 ccgataacgc gatatgagat ccgcaggctc gctgcggcca caggtgcagg tgtcgccgaa
     1201 ggcctttgca gggccgcttt cgtaaggcgt ggcgggccag ggggaacatg cgcgaccgga
     1261 ttcgccgaat gttcgacgat gaaaatatca taaatccagc cgcggattcc cttgtattcg
     1321 acggcgcacc agccccgggt gcacggctgg aaagaagtca gcacggcatt cttggggatg
     1381 gaggcaatga ccggcgattg actggagggg gcgctttgca gcggcgcggc ccggaacgcc
     1441 ttccaggtct ggaaaggctg ctccttcggc ttggccgcgg aggcagttac agctgccagg
     1501 acaaggacag caaggaggct gaacaaagat aataaagata acgttcgggg taatttggtc
     1561 acggccggtt tccctaaagg ctacgctaca aaaaaagcca gagaacgtgt tgcgacttgc
     1621 gcaccggtca cgctttcgcg tggaagtttg gactggttgg aaattcgcat ttccgttcta
     1681 catttgttaa tggatctctg tggctgaaaa atggagcagg gaagcgatgg cattccagca
     1741 ggcgtgtacg cgctgcgcat tagtgttcct gattctttca ctgcaggctg ccttgctgcg
     1801 agcggaagag gcgcagctca aggctgctcc tgagttgtcc gcttccccct ccagcagcgc
     1861 gccatcgccg caggcacggt cagcgcaacc cgagacaagc ggacttcggt cgcaggcctt
     1921 gcttgcttct atcgacaaaa ttctgaagcg ggccgccacg gagcgcgagg aggcaaaaac
     1981 cttgccctct cgcgacaagt tcctgttcga ccctgtttgg acagcgacgc gggaggagcg
     2041 cgaaaaggaa gtccgcgaac tgctcgatgg cgctctctcc gttgtgacgg atgccccgat
     2101 cctgaagctt caagaagaca tccataaaca gcaggacaaa atcgccgcca acagggaccg
     2161 catcgcaacg cttcgcgaga agcggctggg cgttccacaa cccgggctgc tgcccagcat
     2221 tttcaccgaa acgacagagt ccatcgacaa gacgattgcg gcgctcgagg aggatatcaa
     2281 gcggcaggaa gccggcatcg ttcgcatcaa acaggagatc ggcacagcgc ttgctgctgc
     2341 gggagtgacg ctttcgcaag accaactcga tctccttctc gacggcgtgc tcgggggtga
     2401 cctcctgaag ctcatggcgg ccttcgaagt cgcgaagatc gcggacgaga ggctcgcaac
     2461 gcttgtgcag caatccgatg aggatttgaa atcggctcgc cgctattttg ccatgcacgc
     2521 ggcgctcttc gcgatgctgg tacaggccca ggatatgttg atcgagcgaa tcgataagac
     2581 ttatcttgcc cgcctgcgcg aaatcacgag cagtatcgac cagacaggcg cgaaaacacg
     2641 agagttgttg cgctctgccg gccgggagga tcagcgcaag gcgctcgatg ccaatttgaa
     2701 ggcgcaggat gtgtcaagac gggtgtctgt tttctatcgc gactatttgc aggcccaaag
     2761 gcggctcttg gcgcaagcgc gagacaggtc tgttttcgat ttgcgggtgg ccgataatac
     2821 ttatgaaact gtagaggaaa gtttccaatt aaaagcgctc atggatgaag cgcgggcatc
     2881 ttttgaatct gtgcaaaaat tggatcatcc cgggttcgag caaatctttc gtaacgaaaa
     2941 tttaagacgt gaattcgaga acctaaccca gaaactgggg ccctcctcct gatggatgat
     3001 tagctgtcgc cgcgaatcaa caggcggaag ttaattcggt cgtgtgccgt tgtttggcct
     3061 tcgagagagg ctagcttgag gtatatagtg ctgcttctct tatgaagatg ggtgtccacg
     3121 atgaggatcg ttcttgcgct tttactcctc ggagcggttt cgtgcgaagc gctcggtgca
     3181 gaggcggact gcgcctacga tccaaaactg tcccaccttt cgaggatcgt agcagccgac
     3241 tcgacgcatg gtcctatcta cggaacggct ctcaacgacg cttcgaacaa ggcccagagc
     3301 cggacattga acctccgcga tcatgagatc gtcctgacct tcgacgatgg gccgctcggg
     3361 tcgaacacgc agacggtgct cgacacgctg caaaaacatt gcgtgaaggc gacattcttc
     3421 tccgtcggac gtatggcgca gcaaaatccc aagctcctgc aggagttgga ccggaggggc
     3481 catacgatcg gcgcccacac atggtcccat ccgcgggcca tgcatttgct gccggcggac
     3541 gatgcaaaac ttgagatcga gaagggcttt gccgctgtca gtcaggcgct tggcaagccg
     3601 attgcgccgt tcttccggta cccgggcttg agggatacgc ccgaaaccgc ggcctatctt
     3661 gccagccgga atatcagcat ctggtctgtc gatgtggtct caggcgatac ggaaccaggg
     3721 gcgacgtctg cgcgcgtcgc cagcaacgtg ctgtcgcgcg tcaagcaggt cggcaaaggg
     3781 atcatcctct tccacgacat caagaagccg accgccgagg cgctggatgg cattctcacc
     3841 cagcttgaaa aagatgggta caaggtcgtt catgtcgtct ccaatacgga atatcagccc
     3901 aacccggaat tgatcgcgcg cgccgatgcc ttccgcacgg cgcctgagaa agcgacgatc
     3961 accggaacct gggtcgcttc agcgaagcct gagaaggaga aggccgggaa ggaagacatc
     4021 aaggacggca gcgttgacgt catgcacacc gaatggatcg acctgaagca tataagcgac
     4081 atgctggcag gtcaggccag cgatgtggcg agcgcgcgcg cagaccagca gggcgacggc
     4141 ctcacggcaa atagttggac cgcttccggt cacgtccgtt aaccgtagaa gccgcctcag
     4201 cgggcgaatt ctgggggcgc ttttcgcgcg ggacttgttg gtggctttct tgttcaccta
     4261 ttgtcgcgcc tgagcaatcg gggcggcata gcgcattcat gaaacaagac gaagctctcc
     4321 aaatcctgaa aaccggcgcg agcgcgtttc tgacaggcga accgggaagc ggcaaaagct
     4381 atgtagcctc tcgctt
//
LOCUS       DATMGX010001000         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_87655, whole genome shotgun sequence.
ACCESSION   DATMGX010001000 DATMGX010000000
VERSION     DATMGX010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87655"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..390
                     /locus_tag="VNR65_16400"
     CDS             115..390
                     /locus_tag="VNR65_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HWJ20297.1"
                     /translation="MLWTWDEKKNGANRKKHGLSFETAQLVFDDPLAVSRLDPFPLEE
                     RWRTIGAIGPVVVIVIHTSLETSPETGEEFGRIISARKATLRERQAY"
     gene            371..685
                     /locus_tag="VNR65_16405"
     CDS             371..685
                     /locus_tag="VNR65_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HWJ20298.1"
                     /translation="MKDKPTKTATSKLTASQEAELDALAELPDHKIDTSDIPEVRDWS
                     GAMRGAFYRPIKQQLTLRIDADIVAWFKERTRDGKGYQTIINKALREYVERHAKENAK
                     RL"
     gene            873..2531
                     /gene="ettA"
                     /locus_tag="VNR65_16410"
     CDS             873..2531
                     /gene="ettA"
                     /locus_tag="VNR65_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HWJ20299.1"
                     /translation="MAPPQYVYVMQKLSKTYPGGKQVLNNISLSFLPDAKIGVVGVNG
                     SGKSTLLKVMAGVETEHQGEAWAADGVRVGYLPQEPKLDETKDVLGNAMEGVAEKKAM
                     VDRYNELAMNYSDETADEMARLQDQIDGHNLWDLDAQVEQALQAMNCPPPHAEVKNLS
                     GGEKRRVALTALLLARPDILLLDEPTNHLDAESTDWLQHYLEDYPGCVILVTHDRYFL
                     DSITQWTLELDRGQGVPYKGNYSSWLEQKAKRAAQENREDEAKQRTMQRELEWIRASP
                     KARQAKSKARIQAYEKLAEEAGRDQVGKAQIQIPMGPRLGGVVLEADHIKKGFDGRTL
                     IEGLDFKLPPGGIVGVIGPNGAGKTTLFQMILGKDKPDEGAFRIGDTVKLGYVDQSRD
                     ALDPKKNVWEEISGGLDQIKLGTREVNSRAYCGWFNFKGPDQQKKVGQLSGGERNRVH
                     LAKMLKEGGNLILLDEPTNDLDVETLSSLEAALEDFPGCAVVISHDRFFLDRLATHIL
                     AFEGDGHVEWFEGNFQAYEEDKLRRLGAAALDTPKAKFARFARG"
     gene            complement(2607..3002)
                     /locus_tag="VNR65_16415"
     CDS             complement(2607..3002)
                     /locus_tag="VNR65_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20300.1"
                     /translation="MRHLIALIGLTLLTTPLPAQERKVTIVQIWENFLASSVAHNKCG
                     TKDEALEKKFAMNLFEVTIRATQKVKEEKPENPEAKLVKLMDNKGKEIRAKVEEIAAS
                     KGCDFDGIQRLLKLYKFHAEWDMHKSAGR"
BASE COUNT          698 a          883 c          960 g          620 t
ORIGIN      
        1 gtaaggttca tcactttcca agacgcgcga acgtacgtaa aagtttttcc tcgtttgctg
       61 tgcttgacga acgacaatac tgtagctaca attttgtagt ctaaggatac aattgtgctt
      121 tggacctggg acgagaagaa aaacggcgcc aatcgaaaga agcacgggct aagcttcgag
      181 accgctcaac ttgtttttga tgatcccctt gcagtctcac ggctcgatcc ttttcctctc
      241 gaggaacggt ggcgaacaat cggggcgatt ggcccggtgg tggttattgt gatccatacc
      301 tcgcttgaaa cgagcccgga gaccggcgag gaatttggta ggatcatcag tgcgcgaaag
      361 gcgacgttgc gtgaaagaca agcctactaa gacggcgact tctaaactca ctgcctctca
      421 agaggccgaa ctcgatgcgc ttgccgagct tcccgatcat aagatcgaca cgtccgacat
      481 tccggaggtg cgcgactggt ctggggccat gcgcggggcg ttttatcgcc ctataaagca
      541 gcagcttacg ctacgcatcg atgcggatat cgtcgcttgg ttcaaggaac gaacgcgcga
      601 tggcaaaggc taccagacga tcataaacaa ggcgctgcgc gagtatgtcg agcggcatgc
      661 aaaggaaaat gccaaacgct tatgatcttc ggcgttagga atttgtggcc ggctcacaag
      721 cgtgaagtaa cgctgccttg agccgcagcc tccaatccgc tacataagca tcctgatttc
      781 cgcgtcttcg cgtacgtcaa gcgccgccat cacccttgcg gccacgcatc catatcgcga
      841 aacgccaaac gcaatccaac gaaggaaacc tcatggcccc tccgcaatac gtttacgtga
      901 tgcagaagct ttccaagacc taccccggcg gaaagcaggt gctgaacaac atctcgctct
      961 ccttcctgcc cgatgcgaag atcggcgtgg tgggcgtgaa cggctcgggt aagtctacgc
     1021 tcctgaaggt catggcaggc gtggagacgg agcaccaggg cgaggcctgg gccgcggatg
     1081 gcgtgcgcgt gggctatctc ccgcaggagc cgaagctcga cgagacgaag gacgtgctcg
     1141 gcaacgccat ggagggcgtg gcggagaaga aggccatggt cgaccgctac aacgagctgg
     1201 cgatgaacta ttccgacgag accgccgacg agatggcgcg tctgcaggac caaatcgacg
     1261 ggcataattt gtgggacctt gatgcgcagg ttgagcaggc gctacaggcg atgaattgcc
     1321 ccccgccgca tgccgaggtg aagaacctgt cgggcggcga aaagcgccgc gttgcgctga
     1381 cggcgcttct gctggcgcgc cccgacattc tgctgctcga cgagccgaca aaccatctcg
     1441 acgccgagtc caccgattgg ctgcagcatt acctggaaga ttatccgggc tgcgtgatcc
     1501 tcgtcaccca cgatcgctac ttcctggaca gcatcacgca gtggacgctg gaactcgatc
     1561 gcgggcaggg cgtgccctac aagggcaatt actcgagctg gctggagcag aaggcgaagc
     1621 gtgccgcgca ggagaaccgc gaggacgagg cgaagcagcg gaccatgcag cgcgagctgg
     1681 aatggatcag ggcatcgccc aaagcgcggc aggccaagag caaggcgcgc atccaggctt
     1741 atgagaagct cgcggaggag gccggccgcg atcaggtcgg caaggcgcaa attcagatcc
     1801 cgatgggtcc ccggcttggc ggcgtggtgt tggaggcgga ccacatcaag aaagggtttg
     1861 acgggcgcac gctgatcgaa gggctcgact tcaagctgcc gcccggcggc attgtcggcg
     1921 tgatcgggcc gaatggggcg ggcaagacaa cgctgttcca gatgatcctc ggcaaggaca
     1981 agcccgacga gggcgcgttc cggatcggcg acacggtgaa gctcggctat gtggaccaga
     2041 gccgcgacgc gctggacccc aagaagaacg tgtgggagga gatttccggc ggcctcgatc
     2101 agatcaagct cggcacccgc gaggtgaact cccgcgccta ttgcggctgg ttcaacttca
     2161 aggggccgga ccagcagaag aaggtcggtc agctttcggg cggcgagcgc aaccgcgtcc
     2221 atctcgccaa gatgctgaaa gagggcggca acctcatcct gctcgacgaa ccgacgaacg
     2281 acctcgatgt ggagacgctg tcgagccttg aggcggcgct cgaggatttc cccggctgcg
     2341 ccgtggtcat cagccatgat cgcttcttcc tcgaccggct tgcaacgcac atcctcgcgt
     2401 ttgagggcga cggccatgtt gagtggttcg agggcaactt ccaggcctat gaggaggaca
     2461 agctgcgccg cctcggggcc gccgcgctcg atacgccgaa ggcgaagttc gctcgcttcg
     2521 ctcgtggata gcgccaactt gcaacgctcg tgaagaactg attcgcgcac gctttcagaa
     2581 ggcagtcggc cagtatgatt tcttgatcat cggccggcac tcttgtgcat atcccattcg
     2641 gcgtggaatt tataaagctt cagtagtcgc tgtatgccat caaaatcgca gccttttgag
     2701 gcggcgattt cctcaacctt cgcccgaatt tcctttccct tattatccat cagcttgacg
     2761 agtttggcct cgggattttc cggtttttct tcttttacct tctgggttgc tcggatggtc
     2821 acctcgaata agttcattgc gaactttttc tcgagtgctt catctttggt cccgcattta
     2881 ttatgagcga ctgaggaggc caaaaagttc tcccagatct ggacgatcgt gactttgcgc
     2941 tcttgtgcgg gcagaggcgt agttagaagc gtcagcccga tcagtgcaat gaggtgtcgc
     3001 atgcgcattc tccaagtccg atatagaatc tgaccgagaa ttcatagatc gagaacaggg
     3061 gcagcaccac agatgttcgg ggtcaccgga caaattgggc tgccgcgccc ggagctaagg
     3121 aacccatgag tgataaaaag aaagggtagt ctcgccagga a
//
LOCUS       DATMGX010001001         2860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_55495, whole genome shotgun sequence.
ACCESSION   DATMGX010001001 DATMGX010000000
VERSION     DATMGX010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55495"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..1415
                     /locus_tag="VNR65_16420"
     CDS             384..1415
                     /locus_tag="VNR65_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWJ20301.1"
                     /translation="MDHFAGLDVSVKETSICIVDDTGRIVREVKVASEPEALLAVLAN
                     PTYHFKRIGLEAGPLSQWLFSALAEAGLPVICVETRHMRAVLQAQINKTDRNDARGMA
                     QMMRAGLYRPVHVKTLRSQKLRMLLTHRKLLQSKAVAIENDLRGTLRNFGLKVGVVGK
                     VRFEARIRELVENLPDLAVLVEPMLIVRRVLREQIGILHRRLLAIVRDDDVCRRLMTI
                     PGVGPVVALTYRATVDVPARFKNSKAVGAVLGLTPSKYQSGEINRSGAISKCGDEMMR
                     VMLYEAAQVMLTRTNKWSWLKAWAMKIARHRGMKKAIVALARRLAVIMHRIWVDGTEF
                     QWTREAAVA"
     gene            complement(2308..>2860)
                     /locus_tag="VNR65_16425"
     CDS             complement(2308..>2860)
                     /locus_tag="VNR65_16425"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWJ20302.1"
                     /translation="APETDQKNLELLKEMLPKTRRVAMIFDPKNRGMMLRLNTIKTAA
                     LKLAIEIQSIPALSPNELAGALTAAAQDPPDALIVLSPIYAAYRNEVLEFASKTKVPL
                     SFDTRGLAGEPGALLSYGVDISVLFRRAATFVDKILKGAKPADLPVEQPTKFELVINL
                     KTAKALGISIPSTLLARADEVIE"
BASE COUNT          609 a          779 c          856 g          616 t
ORIGIN      
        1 atgcacagca tcggggtatg aaggatatga tgatttatat tcaactccct ctgaagccgt
       61 cgcccgcttg tggttggcct tgaataagaa ggtatgacgg ctctcgcgcg tcaattccgc
      121 cgcatcttcc tgcgcgatgc cgcgtgcgtc gttgcggtcc gtcttgttga tctgcacctt
      181 caacaccgaa tgtccgttct gtgtcattag cgaccgacac attcagcacg agcgcccatt
      241 aatgtccgct gttactccaa tagatggggt aatcggccgg cgatcttgtg gatagctgaa
      301 gattttgggt gctgcgcttc aggctgatgg tgaaacgagg tccgtggtga cggcctcaag
      361 catcagaagg agaagcgtga gccatggacc attttgctgg attggacgta tcggtcaagg
      421 agacgagtat ctgcattgtg gatgacacgg gcaggatcgt gcgggaagtg aaggtggcga
      481 gcgaacctga agctttgctg gcggtgctgg cgaaccccac ctaccacttc aaacggatcg
      541 gactggaagc cgggccgcta tcgcaatggc tgttcagtgc actcgccgaa gccggcttgc
      601 cggtgatctg tgtcgagacg cggcacatgc gggcggtgtt gcaggcgcag atcaacaaga
      661 ctgaccgcaa cgacgcacgc ggcatggcgc agatgatgcg ggcgggactt taccgcccgg
      721 tgcatgtgaa gacgttacgc agccagaagc tgcgaatgct gctgacccat cgcaagctgc
      781 tgcagtcgaa ggccgtcgcg atcgagaacg acctgcgcgg cacgttacgc aactttggtc
      841 tcaaagtagg cgtggtgggc aaggtaagat tcgaggcgcg catcagggag cttgtcgaga
      901 acctccccga cctggcggtg ctggtcgaac cgatgctcat tgtccggcgg gtgcttcgcg
      961 agcagatcgg catcctgcat cgccgtttgc tggccatcgt gcgggacgat gacgtgtgcc
     1021 gacgcctgat gacgatcccc ggcgtcggcc cggtggtggc gctgacctat cgcgccaccg
     1081 tcgacgtgcc ggcccgcttc aagaactcca aggctgtcgg ggcggtgttg gggctgacgc
     1141 cgtccaagta tcaatcgggt gagatcaacc gatccggcgc gatatcaaag tgcggagacg
     1201 agatgatgcg ggtgatgctc tacgaagcgg cccaggtcat gctgacccgc accaacaagt
     1261 ggtcctggct caaagcctgg gccatgaaga tcgccaggca ccgcgggatg aagaaggcga
     1321 tcgtggcgct ggcgcgccgg ctggccgtga tcatgcaccg catatgggtt gacggcaccg
     1381 agttccagtg gacccgggag gccgcagtgg catgacagca gctcaggatc acatgcgata
     1441 ggagaaaact cgagctccac caagcgttgg aatgatgtcc ctcgcgggac gatggatgag
     1501 gtgagttcgt ttgtccgctt ggacctgtcg ttcgtctgac ggcaaggccg cccaatagat
     1561 tgttccgcct cgtcttcgaa tcccatgatg gaagggcctt agtgccgatt ccgaagagaa
     1621 gcacgagccc gcgggcgaca tcgacactgc agtggtgcat agtctgaaag cgcttgaccc
     1681 aaaacggccg attagagaag cggacattgc tcgctggctc attacgtaac caccgcgcct
     1741 tgaaatccgc tatcgcttcc tctcggatgg cggcgtaacc gcgctcggag aggaggccgc
     1801 cgcgagggca ttgggctaac gatggaacgg gaaaaatatt tagcttggcg ttgcggcttc
     1861 gtcagccggt tcaaggacgg gttcggcaaa atagtccata agcagaaccg caccgacgat
     1921 aatgaacagc actagcagtt tcatcggaag gcccctgttc cgcgagacga caaacggaat
     1981 tgggcgcgtt atggacgcct ctgattttcg gtcttaatac cgtgcggtcg ttaataaaat
     2041 tgtgcctagc ggtcgaaaat ccgcataaac aaaaatttat ttgtcccaca gttcgcgagg
     2101 ttattggata tcgctgttag tgcgcatcgc ccaactccca tcggtgcgat gtaatcatcc
     2161 acctactgca caaacccgcg ccctacgagg cgcgtttttt tctgcgcccc acgtccgctt
     2221 tacagctgaa aacggacatt gatcagtgcg ttttgcatgt ccgctttgtg ccaaaaacgg
     2281 actcatgcac cgcagcaaac agcggctcta ttcaatcacc tcgtcggcgc gcgcaagcag
     2341 cgttgatgga atgctgatcc cgagtgcttt ggctgtcttg aggttgatga ccagctcgaa
     2401 tttggtcggc tgctccaccg gcaaatcggc cggcttggcg cccttcaata tcttatccac
     2461 gaacgtggcg gctctccgaa ataacaccga gatatccacc ccgtaggaca agagggcacc
     2521 aggttctccg gcaaggcccc tggtatcaaa agacagcggc accttcgtct ttgacgcaaa
     2581 ttccaagacc tcattccgat aagctgcata tataggagaa agcacaatga gagcgtcagg
     2641 agggtcctgg gcggcggctg tcagcgcgcc ggcaagttcg tttggactta gtgccggtat
     2701 tgattgaatc tctattgcga gctttagcgc cgcggtcttt attgtgttca gtctaagcat
     2761 catgccccga ttcttcgggt cgaaaatcat cgccactcgc ctagttttgg gtagcatctc
     2821 cttcagaagt tctaggtttt tctgatctgt ctcaggggca
//
LOCUS       DATMGX010001002         2545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_457079, whole genome shotgun sequence.
ACCESSION   DATMGX010001002 DATMGX010000000
VERSION     DATMGX010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457079"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1088)
                     /locus_tag="VNR65_16430"
     CDS             complement(150..1088)
                     /locus_tag="VNR65_16430"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWJ20303.1"
                     /translation="MAVLLVAEHNNQALSGATAKALTAAKALGAPVHVLVAGSNAEGV
                     AQKAAALSGVDKVLLADAEHLKAQLAEDLAALIVPLMEGYDSLVAVASTTGKNFAPRV
                     AALLDVAQLSDVTAVLSSTQFERPIYAGNAIQTVECSQGKKVLTIRTTTFPAAPEGGS
                     AAIEKIVAPAPNGISQFVGEELSQSERPELTAAKVIISGGRGMQSGENFKLLERIADK
                     LGAAVGASRAAVDAGFVPNDYQVGQTGKVVAPELYIAVGISGAIQHLAGMKDSRVIVA
                     INKDEEAPIFQIADYGLVGDLFQILPEFEAALDSRK"
     gene            complement(1088..1840)
                     /locus_tag="VNR65_16435"
     CDS             complement(1088..1840)
                     /locus_tag="VNR65_16435"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWJ20304.1"
                     /translation="MKILVPVKRVVDYNVKIRVKPDGSGVDIANVKMSMNPFDEIAVE
                     EALRLKEAGHATEVVAVSVGPQKAQETLRTALAMGADRAILYKTDLNLEPLAIAKILK
                     HAVEQEKPGLVILGKQAIDDDSNQTGQMLAALLNWPQGTFAFKVAAKDGALDVTREVD
                     GGLQTVSLKLPAIVTADLRLNQPRYASLPNIMKAKKKPLDEHDAAALGIDIAPKIKVL
                     KTAEPPTRKAGIKVKTASELVEKLRTEAGVLQ"
     gene            complement(2009..>2545)
                     /locus_tag="VNR65_16440"
     CDS             complement(2009..>2545)
                     /locus_tag="VNR65_16440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWJ20305.1"
                     /translation="DVASIRAFKEHVPEQFRLLTYMFVHGSIWHLVGNMVFLFVFGDN
                     VEDALGHVRFIIFYLLCGVIAAVVHSAVTVSPDVPLIGASGAIAGVIGAYVILHPHIR
                     VWVLFPVPVLSFLPLRFSAAFVIGMWIMYQIASGIWLTGEATAWWAHVGGFFAGVLLI
                     VVMRRPGVPLFDTATGVQ"
BASE COUNT          534 a          756 c          717 g          538 t
ORIGIN      
        1 gcgatgccgt tgcccatctg cccggccccg atgatcccca ccgttttgat gggggcctgc
       61 tcccgcccct cgcttctatt gatgaacgga ttaccgcgaa acattggggt tgtggcttgg
      121 accatcgtgc actctgggtt ctgtgcggac tatttccggc tgtcgagcgc cgcttcaaat
      181 tcgggcagga tctggaaaag gtctccgaca aggccataat ccgcgatctg gaaaatcgga
      241 gcttcctcat ccttgttgat ggcaacgatg actcgtgagt ccttcatgcc ggcaaggtgc
      301 tgaatagcgc ccgagattcc gactgcaata taaagttcag gtgcaacaac cttgccagtt
      361 tggccaacct gatagtcgtt cgggacaaag cctgcgtcta cggccgcgcg gctcgcgccc
      421 acggctgcgc cgagcttatc cgcgatccgc tccaggagct tgaagttctc acccgactgc
      481 atgccgcggc caccggaaat gatgaccttg gcggcagtga gttccggccg ttccgattga
      541 gacagttcct cgccgacaaa ctgagagatg ccgttcggcg cgggcgctac gatcttctcg
      601 atggcagccg agccgccctc aggtgcggcc ggaaaagtcg tggtgcggat cgtcagcacc
      661 ttcttgccct ggctgcattc gacggtctga attgcgttgc cggcatagat cgggcgctcg
      721 aattgcgtgg agctcagaac cgccgtcaca tccgaaagct gcgcaacatc gagaagagct
      781 gcaacccttg gcgcgaaatt cttgcctgtc gtcgatgcaa cagcgaccag agagtcgtac
      841 ccttccataa gcgggacgat gagcgcagcc aaatcttccg cgagttgagc cttgagatgc
      901 tcggcatccg ccaggagaac tttgtcgaca ccgctgagag ccgccgcctt ctgcgcgacg
      961 ccttcggcat tcgagccggc gacaagaaca tggaccggcg cgccaagcgc tttcgccgct
     1021 gtaagcgcct tggcagtcgc tccgctcagc gcttgattat tatgctctgc aaccagaaga
     1081 acggccatta ttgcaacact ccagcctcag tgcgcagctt ttccacgagt tcgcttgcag
     1141 tcttcacctt gatgccagcc ttcctcgtgg gtggttcggc cgtctttaac accttgattt
     1201 tgggtgcaat gtcaattcca agcgctgccg catcgtgctc atcgagtggc ttcttcttcg
     1261 ccttcatgat gttcggcagc gacgcatagc gcggctgatt gagccgcaaa tccgctgtca
     1321 cgattgcagg cagcttcaat gagacggttt gcagtccgcc atcgacctcg cgcgtcacgt
     1381 caagcgcgcc atccttggcc gcaaccttga atgcgaaggt gccttgcggc cagttgagaa
     1441 gggcggcgag catctgcccg gtctgattgg aatcgtcgtc gatagcctgc ttgccgagaa
     1501 tgacaaggcc gggcttttcc tgctcgacgg cgtgcttgag aattttcgcg atcgccaatg
     1561 gctcaaggtt cagatcggtc ttgtaaagga tggcgcggtc ggctcccatc gcaagcgcgg
     1621 tcctgagcgt ctcctgtgcc ttttgcggcc ctacggaaac ggcaacaact tcggtcgcgt
     1681 gcccagcttc cttaaggcga agagcttcct cgacggcgat ttcatcgaag gggttcatgg
     1741 acattttgac gttcgcgatg tccacgccgc ttccatccgg tttcacccgg attttgacgt
     1801 tgtaatcgac gacccgtttg acgggtacga gtattttcat gcgaatagtg ccctggctct
     1861 tttatccatc gaatattatg caccgcaccg ctgggtcaac ttacctgatg gtttgatcgt
     1921 attcatttgc agcgccattc agaaaatcaa gcgtatttcg cacctgcgaa agagtcaatg
     1981 cgccccagct tagacattcg gccagctatt actggacacc ggtcgctgta tcgaaaagcg
     2041 ggacaccagg ccgtctcatg acgacgatga ggagcacgcc cgcgaaaaag ccgccaacat
     2101 gggcccacca ggcggtcgcc tctccggtca gccaaatacc gctggcgatc tggtacatga
     2161 tccacatgcc gatgacgaag gccgcggaga accggagcgg caggaacgaa agaaccggga
     2221 cagggaaaag aacccacaca cggatgtgcg gatgcagaat gacataagct ccgatgacgc
     2281 ccgcgatagc gccgctcgcg ccgatcaggg gcacgtccgg cgagacggtt acggcgctat
     2341 gcacgacagc cgcgatcacg ccgcaaagca gatagaagat gatgaagcgg acatggccta
     2401 gcgcatcctc gacattgtcg ccgaagacga aaagaaacac catattccca acgagatgcc
     2461 agatactgcc atgcacgaac atgtaggtca ggagcctgaa ttgttcggga acatgctcct
     2521 taaaggcacg aatgctcgcg acgtc
//
LOCUS       DATMGX010001003         3449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_55590, whole genome shotgun sequence.
ACCESSION   DATMGX010001003 DATMGX010000000
VERSION     DATMGX010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55590"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1561)
                     /gene="nuoN"
                     /locus_tag="VNR65_16445"
     CDS             complement(80..1561)
                     /gene="nuoN"
                     /locus_tag="VNR65_16445"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004440.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HWJ20306.1"
                     /translation="MLLDSYSKAMTMLSYYAPIFPELFIVLASFVLLMWGVYQRRQTG
                     FIISVVSIIVLLGAALLIARHPPVSQELFNGAFVDDRFARFMKILTLAGSAFTLILSF
                     DYMRRERILRFEYPILILLSTVGMMLTISANDLISLYLALEFQSLALYVVAAFHRDHL
                     RSTEAGLKYFVLGVLSSGLLLYGSSLVYGYTGATAFARIAQAVQLHGASIGFIFGLVF
                     MLAGLVFKIAAVPFHMWTPDVYEGAPTPVTVYFASAAKMAAMAMVIRVILGAFPNATE
                     QWQQIVIAISIASMALASFAAIGQTNIKRLLAYSSIGHVGYALIGVAAVAAAPAELKA
                     ECISSVLIYLAIYLAMTVGAFACVLAMRQGRFMSEAIQDLAGISKEQPLYAFLFAMLL
                     FSLAGIPPLAGFFAKFYVFNAAIQAHLYWLAVVGVVLSVIGAYYYLRIVKLMYFDEPG
                     ITFDPMPSSVQFTLAVTAGFAILFFVYPSPLKAAADAASRSLF"
     gene            complement(1571..3097)
                     /locus_tag="VNR65_16450"
     CDS             complement(1571..3097)
                     /locus_tag="VNR65_16450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWJ20307.1"
                     /translation="MKDWPILTTIILLPVVGAFFLAILRGSDEAANRNARWIALWTTL
                     VTFAVSLVLIVQFDPANSGFQFVEHHPWLSNKITYYVGVDGLSLPFVILTTFLLPISI
                     LASWTSITARVKEYMIAFLLLETLMVGVFVSLDLVLFYLFFEGGLIPMFLIIGVWGGP
                     RRVYASFKFFLYTLLGSLLMLLAILAMYGQAGTTEIPTLLKFHFPENLQTWLWFAFLA
                     SFAVKLPMWPVHTWLPDAHVEAPTAGSVILAAILLKMGGYGFLRFSLPMFPEASVTYA
                     PLMFTLGVAAILYTSLVAFAQKDMKKLIAYSSVAHMGFVTLGVFSGTFQGAQGAVFQM
                     VSHGIVSGALFLSVGVVYDRMHTREIAAYGGLADRMPVYAFVFMVFTMANVGLPGTSG
                     FVGEFLSLLGTFRVSSVTALFATFGIILSALYALTLYRHIMFGKLEKPSLAKITDMNP
                     REIAIMAPLVILTIVLGFYPKPVLDTTSGAVKAMIAPYEKLMVVQSDEGGPRLAKLEK
                     "
     gene            complement(3094..>3449)
                     /locus_tag="VNR65_16455"
     CDS             complement(3094..>3449)
                     /locus_tag="VNR65_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWJ20308.1"
                     /translation="VAYLAYIRETWLPAWTVQNFKPIHAFLYNKWYFDELYHWIFVRP
                     TAWLARILWIYGDGMIIDGLGPDGVSARVLAITKRAVRFQTGFVYQYAFVMLVGVAAI
                     VTYFAYLFRGALFKI"
BASE COUNT          899 a          944 c         1059 g          547 t
ORIGIN      
        1 acctgcggag cgcttcttca tttgtcgagt caaggctttc gaagcgcaag accgggatgt
       61 ctacggaagc gctcacggct cagaacaatg accgcgaggc tgcgtccgca gcagccttca
      121 gcgggcttgg atagacgaag aagaggatcg cgaaacctgc ggttaccgca agagtgaact
      181 gcaccgacga tggcattgga tcgaaagtga tacccggctc gtcgaaatac atgagcttca
      241 cgatgcgcaa ataatagtaa gcgccgatga cggatagaac cacgccgacc acagcgagcc
      301 agtacaggtg agcctgaatg gcagcgttga acacatagaa tttcgcgaag aagcccgcga
      361 gcggcgggat tccggcgagt gaaaacagaa gcatcgcgaa caggaatgcg tagaggggct
      421 gctccttcga tatgcccgca agatcctgaa tcgcctcgct catgaaacgg ccctggcgca
      481 tggcaagaac gcaggcaaac gccccaaccg tcatcgcgag gtagatcgcg agatagatca
      541 aaacgcttga aatgcattcc gcctttagtt ctgcgggcgc ggcggccacg gccgcgaccc
      601 ctatcagcgc gtatcccaca tggccaatgg atgagtaggc cagcagccgc ttgatgttcg
      661 tctgtccgat cgcggcgaag gaggcgagcg ccatggaggc gatcgaaata gcgatgacga
      721 tctgctgcca ttgctctgtc gcgttgggga aggcaccgag gatcacgcga atgaccatgg
      781 ccatcgccgc cattttcgca gccgacgcaa agtagaccgt gaccggcgtg ggcgcgccct
      841 cgtaaacgtc cggcgtccac atatggaacg gcaccgcggc tatcttgaaa accagtccgg
      901 ccagcatgaa gacaagcccg aagatgaagc cgatggacgc cccatgcagt tgcaccgcct
      961 gggctatccg ggcaaaggcg gttgcgccgg tgtagccgta gacgagcgag gagccgtaaa
     1021 gcaggagccc ggaggacagc acgccgagca cgaaatattt cagccccgcc tcggtcgagc
     1081 gcaaatgatc acgatggaag gcggcgacga catacagcgc gaggctctga aactcaagag
     1141 cgaggtaaag cgagatcagg tcattcgccg aaatggttag catcatgccc acggtcgaga
     1201 gcaggatgag gatcgggtac tcgaagcgca ggatgcgttc gcgccgcatg tagtcgaacg
     1261 aaaggatgag cgtaaaggcc gagcccgcga gcgtcaggat tttcatgaag cgcgcgaagc
     1321 gatcatctac gaaagcgccg ttgaacagct cctgggacac gggaggatgg cgcgcgatca
     1381 aaagagccgc gcccagcaga acgatgatcg acacgacgct gatgatgaag ccggtctgac
     1441 gccgttggta aacgccccac atcagcagaa cgaaggaggc caggacgatg aacagttcgg
     1501 ggaagatcgg cgcgtaatag gagagcatcg tcattgcctt ggagtacgag tcgaggagca
     1561 tcgataccgc ctatttctca agcttcgcga ggcgcggtcc gccctcgtcg ctttgcacga
     1621 ccatgagctt ctcataagga gcgatcatcg ctttcacggc gccggaagtc gtatcgagta
     1681 cgggcttcgg gtagaagccg agcacgatcg tcaagataac aagcggcgcc atgatcgcga
     1741 tctcgcgcgg gttcatgtcg gtgatcttgg cgagcgatgg cttctccagc ttcccgaaca
     1801 tgatgtgccg gtaaagggtc agcgcataca aagccgagag gatgatcccg aaggtcgcga
     1861 aaagcgcggt aacgctgctg acgcggaatg tgccgagaag cgacaggaac tcaccgacaa
     1921 aaccgcttgt cccaggcagc ccgacgttcg ccattgtgaa gaccatgaac acgaaagcat
     1981 agaccggcat ccggtcggcg agcccgccat aggctgcgat ttcgcgcgtg tgcatgcggt
     2041 cgtagacgac gccgaccgaa aggaagagcg ctccggagac aataccgtgg ctaaccatct
     2101 ggaagaccgc gccctgagcg ccctggaatg tcccagaaaa tacgccgagc gtgacgaagc
     2161 ccatatgcgc aaccgatgaa taagcgatca gcttcttcat gtccttctgc gcgaaggcca
     2221 ccagcgaggt ataaagaata gcggccacgc caagcgtgaa catcagcggc gcgtaagtga
     2281 cggaggcttc cggaaacatg gggagcgaaa accgcaggaa gccgtaaccg cccattttca
     2341 ggaggatcgc ggccaagatc acggaccccg ccgttggcgc ctcgacgtgc gcgtcaggca
     2401 gccaggtgtg aaccggccac atgggaagct tcacggcgaa tgaggcgagg aaggcgaacc
     2461 acagccaagt ttgcaaattt tccgggaaat ggaatttcag aagcgtgggg atctcggtcg
     2521 tgcctgcctg gccgtacatg gcgaggatcg ccagcagcat caaaagcgag ccgagcagcg
     2581 tatagaggaa aaacttgaag ctcgcataga cgcggcgcgg ccccccccag acaccgatga
     2641 tgaggaacat cgggataagc ccgccctcga agaaaagata aaagagcacg aggtcaagcg
     2701 agacgaacac gccgaccatg agcgtttcga gcaggagaaa cgcgatcatg tactccttca
     2761 cgcgcgccgt gatggaggtc caactcgcta ggatcgagat gggcaaaagg aaggtcgtga
     2821 ggatcacgaa gggcaaggaa agcccgtcaa caccaacgta ataggtgatc ttgttgctga
     2881 gccaggggtg atgttcgacg aattgaaagc cgctgttcgc cggatcgaac tggacgataa
     2941 ggaccaacga aaccgcgaag gttaccaagg ttgtccaaag cgcgatccag cgcgcgtttc
     3001 tgttcgcggc ttcgtcactg cccctcagga tagccaggaa aaacgcgcct acgaccggca
     3061 aaaggatgat cgtggtgagg atgggccaat ctttcatatc ttgaacaaag ccccgcggaa
     3121 gagataggcg aaataggtga cgatagccgc cacgccgacg agcatgacga aggcgtattg
     3181 atagacaaaa cctgtctgaa accggacggc gcgcttggtg atcgcgagca cacgggccga
     3241 aaccccatcc ggacctagcc cgtcgatgat catgccatcg ccgtaaatcc aaagaatgcg
     3301 ggcaagccaa gctgtcggcc gaacgaaaat ccagtggtac aactcatcga aataccactt
     3361 gttataaagg aaggcatgga tgggcttgaa attctgcacc gtccaggctg gaagccaagt
     3421 ctcacggatg taagccaggt aggcgacca
//
LOCUS       DATMGX010001004         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_239609, whole genome shotgun sequence.
ACCESSION   DATMGX010001004 DATMGX010000000
VERSION     DATMGX010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239609"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VNR65_16460"
     CDS             complement(<1..660)
                     /locus_tag="VNR65_16460"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HWJ20309.1"
                     /translation="MGANMVRRLIKDGHRCVVFDQLPKAVKDLAKAKAIGASSLADLV
                     KKLDKPRAVWLMVPAAVVDKTISDLLPFLEAGDILIDGGNSYYIDDIRRAKEIAPKGI
                     HYVDVGTSGGVWGFERGYCMMIGGDLKVVKRLDSIFATLAPGIGNIPRTPGREKLGGT
                     SEQGYLYCGANGAGHFVKMVHNGIEYGMMAAYAEGMGILREANVGKRSHDIDAETTPL
                     RE"
     gene            647..940
                     /locus_tag="VNR65_16465"
     CDS             647..940
                     /locus_tag="VNR65_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20310.1"
                     /translation="MLAPIRPKPIMPSWMVGLFFMRLFGARTLSKFSSELWLLFYFCV
                     SAFIADETLGCRLAQCYKVFTPLFASAKGAKVLMAKNVFIRSFSRACLGAIAS"
     gene            1040..1717
                     /locus_tag="VNR65_16470"
     CDS             1040..1717
                     /locus_tag="VNR65_16470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ20311.1"
                     /translation="MKRITVLLAEDHTVVREGFRKILELEDDFEVVGEAEDGRQAVAM
                     VKKLHPAVVVMDIAMPVLNGLEATRQILKAVPSTRILILTAHNDDAYVKSATESGAVG
                     FLLKQAPARVLSEGIREVNKGHTFFGPFIAKLLPERERKSLDRAGRSKPRKGRLTSRE
                     MEVFQLIAEGKANKQIAAELGIGIKTVETHRERLMEKLNIHSIAGLTRLAVAMGIIEN
                     NVQLTIV"
     gene            2078..>2637
                     /locus_tag="VNR65_16475"
     CDS             2078..>2637
                     /locus_tag="VNR65_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20312.1"
                     /translation="MNATAIRDENVFDATMNAVIIYDEFDYAAKANAMLERAAHRTDE
                     TTRWGVRLWRVDVLKLPPAAEAALAEAVEAHLIMLAVRQVQSLLPWLMDWLERWAMRR
                     QIQEAALAVWGGGSADTRLARASPELSQFAGHHGLSLIFDDNALAEDESSIFASDLHQ
                     REVALTPTLQHILELSERGYQHWGLN"
BASE COUNT          579 a          740 c          729 g          589 t
ORIGIN      
        1 ctcgcgaagc ggcgtggtct cggcgtcgat gtcatgcgac cgcttgccga cattcgcttc
       61 gcgcaagatg cccatccctt cggcatacgc agccatcatt ccgtattcga tgccgttgtg
      121 aaccatcttg acgaaatggc cggcaccgtt cgcgccgcaa tacagataac cttgctcgga
      181 tgttccgcca agcttctcgc gtcccggcgt gcgcgggata ttgccgatgc ccggtgcgag
      241 cgtcgcaaaa atagaatcaa gtcttttgac cacttttaga tcgccgccaa tcatcataca
      301 atacccgcgt tcaaagcccc acacgccgcc gctggttccc acatcaacat agtgaattcc
      361 tttcggcgca atttccttcg cgcgccggat gtcatctatg taataggagt tgccgccatc
      421 aatgagtatg tcgcccgctt caagaaaagg cagaaggtcg gaaatggttt tatctactac
      481 ggctgccggc accatcagcc agaccgcgcg cggcttgtcc agttttttga cgagatcagc
      541 gagtgaagaa gctccgatcg ccttggcctt ggccaaatct ttcactgctt tcggcaactg
      601 gtcgaagacc acgcaacgat gcccatcttt gatgagccgc cgcaccatgt tggcgcccat
      661 ccggcccaag ccaatcatgc caagctggat ggttggttta ttcttcatga gattgtttgg
      721 cgcgcggacg ctttcaaaat tcagcagtga gctatggttg ctcttttatt tttgcgtttc
      781 tgcttttatc gcagatgaga ctttaggttg caggctggcc cagtgctata aggtgtttac
      841 cccattgttc gcgtcggcca aaggagccaa agtcctcatg gcgaaaaatg tattcatccg
      901 atctttttca cgcgcttgtc tgggcgcgat tgcaagttag tcgggtaaaa ctttgcctgc
      961 tggacagcaa tgacgcggcg aactttctgc gcacggaggg attgctaatg gggtcagttg
     1021 aatcccaaac tgacagtcta tgaaacgaat cactgttctg ctggctgaag accacacggt
     1081 cgtgcgtgag ggttttcgca agatattgga gttggaagac gacttcgaag tggtcggtga
     1141 agcggaagac ggccgccagg cggtcgccat ggtcaaaaaa cttcaccccg cagtggtcgt
     1201 catggacatc gcgatgccgg tgctcaatgg tttggaagcc acacgtcaga ttctcaaagc
     1261 cgtccccagc accagaatac tcattctcac ggcgcacaat gatgacgcgt atgtcaaaag
     1321 cgcaacggag tccggtgccg tgggatttct gctcaaacaa gcccccgccc gcgttctgtc
     1381 cgaggggatt cgggaagtca acaaggggca tacctttttt ggcccgttca ttgcgaagct
     1441 tcttcccgaa cgcgagcgaa agtcgttgga tcgagccgga cggtcgaagc cgcgcaaggg
     1501 ccgcctgact tcgcgcgaga tggaagtgtt ccaattgatt gccgaaggca aagccaacaa
     1561 acaaatcgcg gcggaactgg gcatcggcat aaagaccgtc gaaacacatc gcgaacgtct
     1621 catggagaag ctcaacatcc acagtatcgc cggactcacc cgcctcgccg tcgccatggg
     1681 catcattgag aacaatgtcc agttgaccat tgtttaacaa cggaagtttt ttggagtttc
     1741 tccgcctcgc tccgacttgt cctggtcgcg tcaggtttcg ctctccaccc ttccgacctg
     1801 ccgtgtcgag gctggattca tcaactctgg catgggaaaa caccccgcaa cacaaggcgg
     1861 agccgccgtt tcaaaccgtg ccgcccgtca cgctcttctc cagcttaaaa tccctcacat
     1921 tccggctctg ggatgaggag cggcacgggt tggttggttt cagccatctt tgaaatttcc
     1981 gggtaacacc ccatagcgtc cgtgcggcta tccggattat tttcgcattt atgaatgcac
     2041 cagcgatgag ccgctgataa aaccggagat gaaacgtatg aacgcgactg caatacgaga
     2101 cgaaaatgtt ttcgacgcga caatgaatgc ggtgatcatt tacgacgaat tcgattacgc
     2161 ggcgaaagcg aacgcgatgc tggagcgtgc ggcgcatcga acggacgaaa ccacgcgttg
     2221 gggtgtcagg ctgtggcgcg tggacgtgct gaaactgccg ccagcggccg aagcggcgct
     2281 ggcggaagcg gtggaagcgc acctgatcat gctcgccgtg cgccaggttc agtcccttct
     2341 gccgtggctg atggactggc tggagcggtg ggccatgcgc aggcaaatcc aggaggccgc
     2401 actggccgta tggggtggtg gaagcgccga cacacgtttg gcgcgagcct cacccgagtt
     2461 gtcgcaattt gccgggcatc atggcttgag cctcatattt gacgacaatg cgctggccga
     2521 ggacgaatct tcgatatttg cgagcgacct gcaccaacgc gaggttgctt tgaccccgac
     2581 actccagcac atcttggaac tgtcagaacg cggctatcaa cattggggcc tcaacga
//
LOCUS       DATMGX010001005         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_239664, whole genome shotgun sequence.
ACCESSION   DATMGX010001005 DATMGX010000000
VERSION     DATMGX010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239664"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VNR65_16480"
     CDS             complement(<1..850)
                     /locus_tag="VNR65_16480"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWJ20313.1"
                     /translation="MDQFALANDFPPASEADWRALVAKALGGAPFEALETPLYEGFRT
                     EPLYTKRGEAPPISGNRGWSIIQPLVGGKQFADDLTGGASAFSVDFDACSGIANKDDL
                     EPLVGSANESFFIVPGSSIADAALLLASKGDGLAGSAGFDPLTAFALSGERPAERSAL
                     FADYADAAFTIRERNPAFVPFLASGHAWNGAGGSATEELGFTLAAGVAYWRALTQAGM
                     PLTDAARCIGFSLSASSDIFITIATLRAMRLVWTRALAAAGENPNQDLLLLAKMPHRV
                     LTAYDPH"
     gene            1083..>2498
                     /gene="carB"
                     /locus_tag="VNR65_16485"
     CDS             1083..>2498
                     /gene="carB"
                     /locus_tag="VNR65_16485"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWJ20314.1"
                     /translation="MPKRTDIDSILIIGAGPIVIGQASEFDYSGTQACKALRCEGYRI
                     VLINSNPATIMTDPDLADATYIEPITPDMVAKVIEREKREHPGERMALLPTMGGQTAL
                     NTSLRLARDGTLERFGVEMIGAKAPAIDKAEDRQLFREAMRKIGLATPKSMLAHNISE
                     AMAALDFVPLPAIIRPSFTLGGTGGGIAYNREEYIYIAERGLEASPTTQVLIEESVLG
                     WKEYEMEVVRDTKDNCIIICSIENVDPMGVHTGDSITVAPALTLTDREYQMMRNASIA
                     VLREIGVETGGSNVQFAVNPADGKLVVIEMNPRVSRSSALASKATGFPIAKVAAKLAV
                     GYTLDELDNDITKGATPASFEPTIDYVVTKIPRFAFEKFPGAPTTLTTSMKSVGEVMA
                     IGRTFHESLQKALRSMEIGLSGLDDFVLEGLGKGDDKNVIREALGTPSPDRILKVAQA
                     LRMGVDHEQIHTLCRIDPWFIS"
BASE COUNT          538 a          724 c          779 g          457 t
ORIGIN      
        1 cgtgcggatc gtacgcagtg aggacgcgat gcggcatttt agcgaggagg agcaagtcct
       61 gattcgggtt ttctcctgcg gcagcgagcg ctcgcgtcca tacgagacgc atcgcacgga
      121 gcgtcgcgat ggtgatgaag atgtccgatg atgccgagag cgaaaatcct atgcagcgcg
      181 cagcatctgt aagcggcatt cccgcctgag tgagcgcgcg ccaataggcg acccccgcag
      241 ccagcgtaaa gccgagttct tcggtagccg agccgcctgc tccattccag gcatggccgc
      301 tcgcgagaaa cggcacaaaa gcggggttgc gctcgcgaat ggtgaaagcc gcatcggcat
      361 aatcggcgaa gagcgcggac ctctcggcgg gacgttcgcc ggaaagcgcg aaagccgtga
      421 gagggtcgaa gccggccgaa cccgcaaggc cgtcgccctt ggaagccagg aggagagcgg
      481 cgtctgcaat ggaagagcct ggcacgatga aaaaggactc gttcgccgag ccaacgagag
      541 gttccagatc gtccttgttc gcgatgccgg aacaggcgtc gaagtcgaca gaaaaagcgg
      601 atgcgccgcc agtcaaatcg tccgcgaact gtttcccgcc aaccagcggc tgaatgatcg
      661 accagccgcg attgccggaa atcggcgggg cttcgccgcg cttcgtgtaa agcggctcgg
      721 ttcgaaatcc ctcgtagagc ggagtttcga gcgcttcgaa gggggcgccg ccgagagcct
      781 tggccacaag tgcgcgccaa tcggcttctg atgccggggg aaaatcgttt gcgagagcga
      841 attgatccat ggtgcgcatg ataatggatg cggctacgct gcgctacccg ctcgataacc
      901 ttgcatagct gccttttgga cgcgccctta caacttttac gcaggacgat tacgtcgaat
      961 acaagtctgc aagcaggctt gaaacaagtg caatgatgcc attctgacgc gctcaatctt
     1021 aagctgttgt tgaaaccctc gctgcttcct ttataacgcc tcgaaacccg agcgcttaaa
     1081 gtatgcccaa acggactgat atcgactcca tcctcatcat tggggctggc ccgatcgtga
     1141 tcggccaggc tagcgaattc gactattccg gcacgcaagc ctgcaaggcg ctgcgctgcg
     1201 agggatatcg aatcgttctg atcaattcga atcccgccac gatcatgacg gacccggatc
     1261 tggcggatgc gacctatatc gagccgatca cgccggatat ggtcgccaag gtgatcgaac
     1321 gggagaaaag agaacatccc ggcgagcgca tggcgctgct gccgaccatg ggcggccaga
     1381 cggcgctcaa cacatcgctt cggttggctc gtgacgggac gctggagcgg tttggcgtcg
     1441 agatgatcgg cgcgaaagct ccggccatcg acaaggccga agaccgccag cttttccgtg
     1501 aagcgatgcg gaagatcggg ctcgcgacgc cgaagtccat gctggctcat aatatttcgg
     1561 aagcgatggc ggcactcgat ttcgtgcctc ttccggcaat catccggccg tccttcactc
     1621 tcgggggaac gggcggaggc attgcgtaca accgcgaaga gtacatctat atcgccgagc
     1681 gcggcctcga agcctcgccc acgacccaag tcctgatcga ggaatcggtc ctgggctgga
     1741 aggaatatga gatggaggtt gtccgcgaca cgaaggacaa ttgcatcatc atatgctcca
     1801 tcgaaaacgt cgatccgatg ggcgttcata ccggcgacag catcacggtc gcgccggcgc
     1861 tcacgctgac ggatcgcgaa tatcagatga tgcggaatgc ctcgatcgcc gtgctgcgcg
     1921 agatcggcgt ggagacgggg ggctcgaacg ttcagttcgc ggtcaatccg gcggacggca
     1981 agctcgtcgt gatcgagatg aatccgcgtg tgtcgcgttc atccgctctg gcgtcgaagg
     2041 cgaccgggtt cccgatcgcg aaggtcgcag cgaagctcgc tgtggggtac acgctcgacg
     2101 aactcgacaa cgacattacg aagggcgcga cgcccgcgtc gttcgagccg actatcgatt
     2161 atgtggtgac gaagatcccg cgcttcgcct tcgagaaatt tccgggcgcg cccacgacgc
     2221 ttacaacctc catgaaatcc gtgggcgaag tgatggcgat tggccggacc ttccacgaaa
     2281 gcctccagaa agcgttgcgg tccatggaga tcggtctttc agggctcgat gatttcgtgc
     2341 tggaagggct tggcaagggc gacgacaaga acgtcatccg ggaagccctc gggacgccat
     2401 cgccggaccg catcctgaaa gtggcccaag cgctccgcat gggcgtcgat cacgagcaga
     2461 tccataccct ctgccgtatc gatccgtggt tcatctcg
//
LOCUS       DATMGX010001006         3957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_305749, whole genome shotgun sequence.
ACCESSION   DATMGX010001006 DATMGX010000000
VERSION     DATMGX010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305749"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2352)
                     /locus_tag="VNR65_16490"
     CDS             complement(<1..2352)
                     /locus_tag="VNR65_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWJ20315.1"
                     /translation="MTLAAGTRLDCYEILAPLGTGGMGEVYRARDSRLGRTVAIKVMR
                     SGLSTDEPSRKRFVREARAIAALNHPGIAALYDAGEAGDGLYLAMEFLEGPTLQQEIG
                     HGTISSSTLREYSLQIAAALEHAHAHGIIHRDIKPANLIITRNGVLKLLDFGLARNTA
                     PTEETPSMVTGAGAIVGTLQYCAPEVLAGRPATIRSDIYSLGVAMYEMACGQVPLAGL
                     DTPSLISACLGGQIPPVRQRNPALPEDLAIVIERAMALAPHDRFQSAAELAAALRSNG
                     GRPNKSAIFIERAAPVIAVFDFDNLSGDSATEWLGTGIAETICADLRKLGKVQVVSRD
                     RVQMELHRSGDNKDMTVLGTHLNARWLVMGSYQRAGDRIRITPKLLEPVSGEAITVGK
                     IDGAWNEIFDLQDRVVSEIIRTLELEIDSGARQRIAAPETLRLEAYEQYVQGLKNYQV
                     LGKDSIEASRKHYERAIELDPDYAVAYSGLGQAYAMRWIYRNDPDDLARAAGCLERAL
                     ELDPEMGAPFGILCYVYSRQNKLEKAMDAGVRAVHHDPDAYLPHYYLGAAYWMASQGI
                     SSYLQNAVDNFLISVQIEPLLSSSWLNLGAIAMETSAYDRAEQLVAEVLVLQRSKRAI
                     SPLPFAEMILAGVAMRRLDWEKALEWHERGRHHLAQIDHVYRETAIALNACGMADVRL
                     RQNQPDRALAELHRAWRIAGEYPRMMAHNRVVARTAAGMASAYAALDERGKAEQFVND
                     AASRLEIVYQNRGGLIHGVATSELCRGLAVAQIRLGNLDAASAL"
     gene            2668..3564
                     /locus_tag="VNR65_16495"
     CDS             2668..3564
                     /locus_tag="VNR65_16495"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HWJ20316.1"
                     /translation="MTAGDRLLWIGLSRIWRDWRSALAIIKPETVIAWHRAGFRRFWT
                     WKVRRGRPGRPVISREIRDLIRRMCRENPIWGAPRIHGELLKLGIDIGETSVSKYMVR
                     SRKPPSQTWRTFLENHLTQLVSIDFFTVPTIRFQVLYVFLVLGHDRRRILHCNVTAHP
                     TAEWTGQQLREAFPFDQVPRYLLRDHDAIFGQDFREQVRDMGIHEVLSAPRSPWQRAY
                     VERVIGSIRRECLDHLIVFNETSLRRTLNLYFAYYHRSRTHLSLGKDSPVPRPIQPPA
                     AGPVVALPQVGGLHHRYERQAA"
BASE COUNT          776 a         1241 c         1081 g          859 t
ORIGIN      
        1 cagagcactg gctgcatcaa ggttgcccaa acgaatttgg gcaactgcca gccctcggca
       61 cagttcggag gttgccactc cgtgtatcag cccgccgcga ttctgataga cgatttccaa
      121 ccgtgatgcc gcatcgttca cgaactgctc agccttgccc cgctcgtcca acgctgcata
      181 tgcggaagcc attcccgccg ctgttcgcgc cacaactcga ttgtgtgcca tcatgcgcgg
      241 atactcaccg gcgatgcgcc acgctcgatg taattcggcc aatgcacgat ccggctggtt
      301 ctgccgcagt cgcacgtccg ccattccgca cgcattcagg gctatagcag tttcccggta
      361 aacgtgatcg atctgagcta ggtgatgccg gccgcgctca tgccactcca gcgccttttc
      421 ccagtcaagc cgccgcattg ccacgccagc gagaatcatt tctgcaaatg gcagggggga
      481 gatcgcccgc ttcgaccgct gcagtacaag cacctcagca acaagttgtt cggcgcgatc
      541 gtacgcggat gtttccatag cgatggctcc gagattcagc caactggaac tcagcagggg
      601 ctcgatctgt accgagatca gaaagttgtc tacagcgttc tgcaagtacg aggagattcc
      661 ttgactagcc atccagtaag ccgccccgag atagtagtgc ggcaaatatg cgtcggggtc
      721 atggtggaca gctctgaccc ccgcatccat ggctttctcc agcttgttct gccgactgta
      781 gacatagcag aggatgccga acggtgctcc catttcgggg tcaagctcga gggcgcgctc
      841 caaacatcca gccgcacggg ccaggtcatc ggggtcgttt cggtaaatcc atcgcatggc
      901 gtacgcctgt cccagccccg aatatgccac ggcgtagtcg ggatccaact cgattgcacg
      961 ctcatagtgt ttccgtgacg cctcaatcga gtctttgcca agtacctgat aattcttcag
     1021 gccctgaacg tattgctcgt aagcttcgag ccttagcgtt tctggcgccg cgatgcgctg
     1081 gcgagcaccc gagtctatct ccaattcgag tgttcggatg atttcgctca ctacccggtc
     1141 ctgcagatca aagatctcgt tccatgctcc atcgatcttc ccgacagtaa tggcttcgcc
     1201 gctgactggc tccagaagct tcggcgtaat cctgatccgg tctcccgcac gctgatagct
     1261 ccccatcacc agccaccgcg cgttaaggtg tgtccctaag actgtcatgt ctttgttgtc
     1321 gcctgagcgg tgaagctcca tttgcacgcg atcgcggctg acgacttgaa cttttccgag
     1381 cttccgcaaa tctgcgcata tcgtttccgc aatcccggtg cccaaccatt cggtggccga
     1441 atcgcccgac aagttgtcga aatcgaagac cgcgatcacc ggggctgccc gttctatgaa
     1501 aatcgccgac ttgttcggac gtcctccatt gctgcgcagg gccgctgcta actctgccgc
     1561 gctttggaaa cgatcgtgcg gcgcgagcgc catcgcgcgc tcgatgacga tcgccaagtc
     1621 ctccggaagg gcgggatttc gctggcgcac cggtggaatc tgtccgccga ggcaggccga
     1681 tatcagagag ggcgtgtcca gtccggcgag tggcacttgc ccacaggcca tttcgtacat
     1741 cgcaacgccc aggctgtaga tgtcgctgcg tatcgttgcc gggcgccccg caagtacctc
     1801 cggcgcgcaa tattgaagcg tgccgacgat tgccccggcg cccgttacca tcgacggcgt
     1861 ttcttccgtt ggcgctgtat ttcgggctag accgaaatcc aggagcttga gcaccccgtt
     1921 tcgcgtgatg atgaggttgg ccggcttgat gtcgcgatgg atgatgccgt gtgcgtgggc
     1981 gtgctccaga gcggcggcga tctgcaggga atactctctc agggtcgaac tcgaaatcgt
     2041 gccgtgaccg atttcctgct gcagggtagg cccctcgaga aactccatcg ccagatacaa
     2101 accatctccc gcctcgcccg catcgtaaag cgccgcaatg cccggatggt tcaaggctgc
     2161 gattgcgcgc gcttcccgga caaagcgttt gcgactcggc tcatccgtag acaagccaga
     2221 tcgcatcacc ttgatcgcca cggttcggcc cagccgcgag tctcgtgcgc ggtacacctc
     2281 gcccattccg ccggtcccta ggggcgcaag gatttcgtag caatccagcc gagtgcctgc
     2341 ggctagcgtc ataggtggcg tggattatac cccaagtcgg acgttgtttt gagtcatgaa
     2401 aaccgagtct gatccagttt gttggctact ccgaagttat cgtcaaatcc acctgtcctt
     2461 ctcaaaaacc tcgaaatgtc tgagcggccg cgatttatac ttccttcaac ttccttcata
     2521 atctctcctc cgatgctcat ttctctagcg tcagtcctcg cgactcttgg ttcggtcttc
     2581 cgctcgcgcg cggtcctcga actcgagaac ttagctttgc gccatcaaat cggtgtgctt
     2641 cagcggtccg cgagaaaacg cccgaaattg accgcaggag accgcctgtt gtggatcggg
     2701 ctgtcccgca tctggcgcga ctggcgctcg gcgctggcca tcatcaagcc cgagacggtc
     2761 attgcgtggc atcgtgccgg ctttcgccgg ttctggactt ggaaggtgcg ccgcggacga
     2821 ccaggacggc cggttatttc ccgcgagatc cgagacctga tccgcaggat gtgccgggag
     2881 aatcccatct ggggtgcgcc ccgcatccat ggtgagctgc tcaaactcgg catcgacatc
     2941 ggtgagacca gcgttagcaa gtacatggtg cgcagccgca agccgccgtc gcagacctgg
     3001 cgcactttcc tggagaacca cctcacccaa ctggtctcca tcgacttctt cactgtcccc
     3061 accatccgct tccaggttct ttacgtgttt ttagtattgg gccatgaccg gcgtcgcatt
     3121 ctgcactgca atgtgactgc ccacccgaca gcggagtgga caggacagca actgcgggag
     3181 gcctttccct tcgaccaagt cccgcgctac ctactccgcg accacgatgc catcttcggc
     3241 caagacttcc gagaacaggt gcgagacatg ggcatccacg aagtgctgtc cgcgccgcgc
     3301 tcgccttggc aacgagcata cgtggagcga gtgattggtt ccattcggcg cgagtgtctt
     3361 gaccatctga tcgtgttcaa cgaaacctcg ttacgacgaa ccctcaattt gtatttcgct
     3421 tactatcacc gatcgagaac acatctctcg ttggggaagg actcgcctgt gccacgaccc
     3481 attcagccgc cggcagcggg gccagtcgtg gcgctgccgc aagtcggcgg tctacaccat
     3541 cgctacgaac gacaggccgc ctgaaagcct gaagctagga tcgcgctatg ttcgcgcacc
     3601 ctttgaaatc tgccaccgaa ctgggacgag gagcacgtgt gctcggttgc aattcaatca
     3661 tccgacagag ctcaggcaca ttctcggaag cgcccgagcc tatctgttta cctggcaagt
     3721 tgcagttgct tgagccgtac ggcattttcg cgagacacag tcggaaaaag gagacccgga
     3781 agctgttgag agagcttccc ggcaattgga cgcgacgctg aagttttgcg caaagaatgt
     3841 cagcgaaaag tatattgcac cacctgccac cacagatttc cgcatcctct tcctttcaac
     3901 ggcgaaaagc aggctcgaca gccattgcgc catcccaata acgcgtgatg ggcacgg
//
LOCUS       DATMGX010001007         2454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_139855, whole genome shotgun sequence.
ACCESSION   DATMGX010001007 DATMGX010000000
VERSION     DATMGX010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139855"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VNR65_16500"
     CDS             complement(<1..1029)
                     /locus_tag="VNR65_16500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWJ20317.1"
                     /translation="MTSIAPIVELIECPQRREPTPPGPCAMVVFGASGDLTARLLVPA
                     LYNLSRTRLIPENFALIGVARSEETSESWRDDLYEKLKSYAEKASKFNGGRVDEEAWN
                     RLADKMSYVQGDLSKPELYDKLRATLAEAEKTHGTQGNVIFYLAVAEQFFCPAIEHLR
                     EAKLTEQPIGEDEKPRYWRRVVFEKPFGHSLESARALNARVLRTLSEDQIFRIDHFLG
                     KETVQNIMALRFANGMFEPIWNRDRIDHVQVTVAETVGVESRGTFYEATGAFRDMVPN
                     HVLALVSMVAMEPPSAFDSASIRSRKADVFTAMRPVRVNQVVRAQYAAGTVAGKPVKA
                     YRDEPNVAR"
     gene            complement(1037..2041)
                     /gene="gnd"
                     /locus_tag="VNR65_16505"
     CDS             complement(1037..2041)
                     /gene="gnd"
                     /locus_tag="VNR65_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HWJ20318.1"
                     /translation="MQIGVIGLGRMGGNISRRLMRAGHSCAVYDANSNASAALAQEGA
                     TAAHSLADLVRKLEEKPRAVWVMLPAGKITEETVAELGRLLEPGDIIIDGGNTFYKDD
                     IRRAKELAAKQVRYVDCGTSGGVWGLERGYCLMIGGPNEAVDHLDPIFSTLAPGAGEI
                     AHTPGREKRDNRAERGYIHAGPSGAGHFVKMIHNGIEYGLMQAYAEGFNILRNKDSKD
                     LPEDERFELNIPDIAEVWRRGSVVSSWLLDLAAAALAKDPALETFSGFVQDSGEGRWT
                     IEAAIEEAVPAEVLSSALFTRFRSRQQHTFGEKMLSAMRFGFGGHVETPQAAPSNASK
                     "
     gene            complement(2228..>2454)
                     /locus_tag="VNR65_16510"
     CDS             complement(2228..>2454)
                     /locus_tag="VNR65_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20319.1"
                     /translation="RGGYGPGWRGGWRPGYGWRSGYGWVPFAAFGAAAAATGAYYYNR
                     PYYGPGPYNYGPGPSYGPCGAVPCDEPPLK"
BASE COUNT          456 a          765 c          750 g          483 t
ORIGIN      
        1 gcgcgccaca ttgggctcat ccctgtaggc cttgacgggc ttccccgcca ccgttcccgc
       61 tgcgtattgc gcacgcacca cctgattgac cctgacaggg cgcatcgccg tgaagacatc
      121 ggccttcctc gaccggatgg aggccgagtc gaaagctgag ggcggctcca tcgcgaccat
      181 cgagaccagc gccaagacat ggttgggcac catatcgcga aaagcgcctg tcgcctcata
      241 gaaggtcccg cggctctcca cgccaaccgt ttccgccacg gtcacttgca catggtcgat
      301 gcggtcgcgg ttccagatcg gttcgaacat gccgttggcg aagcgcagcg ccatgatgtt
      361 ctgcacagtc tccttgccga ggaagtggtc tatccggaag atctgatctt cactgagcgt
      421 ccgcaacact cgggcgttca gcgcgcgcgc cgactccagg ctgtgaccga agggcttttc
      481 gaacaccacg cgccgccagt accgaggctt ctcgtcttca ccaatcggct gctcggtcag
      541 cttcgcctcg cggaggtgct cgatagccgg gcagaagaac tgctcggcga cggcaaggta
      601 gaagatgaca ttgccctggg tcccgtgggt cttttccgcc tcggccaagg tcgcgcgcag
      661 tttgtcgtaa agctcgggtt tgctcaaatc gccttgcaca taggacatct tgtcggcgag
      721 gcggttccaa gcctcttcgt caacgcggcc gccgttgaat ttcgaggctt tttcggcata
      781 gcttttcagc ttttcataaa ggtcatcgcg ccagctttcg gaggtctctt ctgatcgggc
      841 gacgccgatg agcgcgaagt tttcggggat caagcgcgtg cgcgacagat tgtagagagc
      901 cggaaccaag aggcgcgcgg tcaggtcccc gcttgcgccg aagaccacca ttgcacatgg
      961 cccggggggc gttggctctc tgcgctgggg acactctatc aactccacga tgggcgctat
     1021 cgaagtcatt tcggacctac tttgacgcgt tggagggggc cgcttgcggg gtttcgacat
     1081 ggccgccgaa tccaaaacgc atggcggaaa gcatcttttc gccgaaggtg tgctgttgcc
     1141 tcgaacggaa ccgggtaaag agggcggacg agagcacctc ggcgggcacg gcttcttcga
     1201 tcgcggcttc gatggtccag cggccttcgc cggaatcctg aacaaagccc gagaaggttt
     1261 cgagagccgg atccttcgcg agcgccgcgg cggccagatc gagcagccag gaagaaacaa
     1321 cgctgccgcg ccgccagacc tcggcgatat cggggatgtt cagctcgaag cgctcgtctt
     1381 ctggcaggtc tttggaatcc ttgttgcgca ggatgttgaa gccctcggca taggcctgca
     1441 tcagcccgta ttcgatgccg ttatgaatca tcttgacgaa gtgcccggcg ccggatggcc
     1501 cggcgtggat gtagccgcgc tcggcgcggt tatcgcgctt ctcgcgaccg ggggtgtgag
     1561 cgatttcgcc cgctccaggg gcgagcgtcg agaagatcgg gtcgagatgg tccacggctt
     1621 cgttggggcc gccgatcatc aggcaatagc cgcgctcgag accccagacg ccgcccgacg
     1681 ttccgcaatc gacgtaccgg acttgcttcg cggccaactc tttagcgcgc cggatatcgt
     1741 ccttgtagaa ggtattgccg ccatcgatga tgatgtcgcc cggttcgaga aggcggccaa
     1801 gctccgcgac cgtctcttcg gtaattttcc cagcgggcaa catcacccag acagctcgcg
     1861 gcttctcttc gagctttctt accaaatctg ctaacgaatg agcggctgtg gcaccttcct
     1921 gcgcaagggc agcactggcg ttggaattgg cgtcgtatac cgcacaactg tgccccgctc
     1981 tcatcaagcg gcgggaaata ttgccgccca tgcggccaag tccgatcacg ccgatctgca
     2041 tgtgcttcgc tccgttggct ttaaatgggt gcggctctga attcacagat catcgccttc
     2101 ggctcagaca tgtgaaagtc ggatatggga tgccgctaac attcaccaac agagctcacg
     2161 cgaaatcggt ccgtggcatg acgcaagcta ggcaggcagc cgttccgccg gctgcctgcg
     2221 cgccattcta tttgagggga ggctcgtcac acggaaccgc tccacatggg ccataagagg
     2281 gaccggggcc gtaattgtac ggacctggac cataataggg cctgttgtag tagtaggcgc
     2341 cggtggcggc tgcagccgcg ccgaaggccg caaagggcac ccatccgtat ccagaccgcc
     2401 acccgtagcc gggacgccac ccgccacgcc agccgggacc gtacccgcca cgcc
//
LOCUS       DATMGX010001008         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_88409, whole genome shotgun sequence.
ACCESSION   DATMGX010001008 DATMGX010000000
VERSION     DATMGX010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88409"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VNR65_16515"
     CDS             <1..187
                     /locus_tag="VNR65_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20320.1"
                     /translation="FPWGALARLGLARAYALYAAKDPAGREQARTAYQNFLTLWKDAD
                     PDIPIYKQAKAESAKLQ"
     gene            561..1940
                     /locus_tag="VNR65_16520"
     CDS             561..1940
                     /locus_tag="VNR65_16520"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWJ20321.1"
                     /translation="MTVAALLPQPWNKRLIDTNVESLRDSDLAWADVALIGGMHMQQE
                     DLISIVQRCRARGLRTVVGGPITSSVPASVLQADHVVIGEAEDLIAGLARDLEAGTAR
                     PYYQAAERPAMERSPLPDLRLIQMHRYSTMTVQYSRGCPFNCEFCDIIEIYGRRPRTK
                     AVAQVLAELDQLREAGWRDAVFVVDDNFIGNKPRAKALLAAMAEWGEAHGHPFRFSTE
                     ASLNLADDLELLQLMKEAGFISVFLGIETPDEDSLVANHKLQNTRRNLLESIGIIQQY
                     GIEVMGGFILGFDTDREDVFERLVEFIQKSAIPVAMVGLLQAMPGTQLFRRLLSEGRI
                     LHAGGGNNTDCELNFLPRMNATRLLEGYRHVLTRIYACDAYYERVRLFLSRCQPQFRQ
                     RWSYGSLRAFFSSMVHQGLLGKARLSYWKFLLTAATRYRGSFGAAMTLAVMGHHFQTI
                     TDQLSNADS"
BASE COUNT          399 a          679 c          572 g          416 t
ORIGIN      
        1 ctttccctgg ggagcgctgg cccgcctcgg cctggctcgc gcttacgcac tctatgccgc
       61 taaagacccc gccggtcgcg agcaagcccg caccgcgtat cagaatttcc ttaccctctg
      121 gaaagatgcc gatcccgaca tccccatcta caaacaagcc aaagccgagt ccgcgaagtt
      181 gcaatagccg ttattaccac gcaacctttg aacggcgaga ccttaagtcc ctataagtct
      241 tgttcgaggg agcgtcatga tgctgaatgt atgatgaggc tcattgagcc tctgtagtct
      301 ttttgtattt cccattgcgc cctcccgccc cgaaggatca cccttgaatc gagcgcgtat
      361 cgatggttgc agttggcgcg ggcattcccg cccagagcgg atcccgcttc attgctgtta
      421 cacgctgata cccgctcttg cagcggaggc gaaacatgaa cgccttactc gtctatccca
      481 ggtgaccgga cacctattgg agcttccgtt atgacctgtc gcttcaaggt aagcgcgccg
      541 ctcagcctcc gctgggtctc atgactgtag ccgcgctgct tccccaacca tggaacaagc
      601 gcctgatcga caccaacgtc gagtccctga gagactctga cctcgcctgg gccgacgtcg
      661 ccttgattgg cggcatgcac atgcagcagg aggacctgat ctccatcgtg cagcgctgcc
      721 gtgcccgcgg actccgaacc gttgtgggcg gccccatcac cagcagcgtg cccgcgtccg
      781 tactgcaagc cgaccacgtg gtgatcggcg aggcggagga tcttatcgcc ggcctggccc
      841 gagatctgga ggcgggcacg gcacgcccct actaccaggc ggccgagcgt cccgccatgg
      901 agcgcagccc attacccgac ctgcgtctta tccagatgca ccgctacagc accatgaccg
      961 tccagtattc gcgcggctgc cccttcaact gcgaattctg cgacatcatc gagatctatg
     1021 gccgccgtcc gcgcaccaaa gctgtggcgc aggtcctggc cgaactcgat cagttgcgcg
     1081 aggcaggctg gcgcgacgcg gtcttcgtcg ttgacgataa cttcatcggc aacaagcccc
     1141 gtgccaaggc actgctggcg gccatggccg agtggggaga agctcacggt catcccttcc
     1201 gcttcagcac cgaagcatcg ctcaatctgg ccgacgatct cgaactgctg cagctgatga
     1261 aagaggccgg cttcatatcc gtctttttgg gcatcgagac cccggacgag gacagcctgg
     1321 tcgccaacca caaactgcag aacacgcggc gcaacttgct ggaaagcatc ggcatcatcc
     1381 agcagtacgg catcgaagtg atggggggct tcatcctggg cttcgatacc gaccgcgaag
     1441 acgtctttga gcgcctcgtc gagttcattc aaaagagcgc cattcccgtt gccatggtgg
     1501 gcctgcttca agccatgcca ggcacacaac tgtttcgccg cctgttgagc gagggccgaa
     1561 ttctgcatgc cgggggcggc aacaacactg attgtgagct gaatttcctt ccacgcatga
     1621 acgccacccg cctcctcgag ggctatcgtc atgtgctcac tagaatctat gcctgcgatg
     1681 cctattatga gcgggtcagg ctcttcctca gccgctgcca gccgcaattc cggcagcgct
     1741 ggagctacgg cagcctgcgc gccttctttt cttccatggt gcaccaggga ttgctgggaa
     1801 aagctcgcct cagctattgg aagtttctgc tcacagccgc cacacgctac cgcggctcct
     1861 tcggcgccgc gatgaccctg gccgtgatgg gccatcactt ccagacgatc accgaccagc
     1921 tctctaacgc agactcttga tcgaagtcgt ggactcagga atcgatgccc ccaggacgga
     1981 ctctcaacga tgcgggcccg aagtcctctt gtgggaggtt gaaccgggct caccgctatt
     2041 tgcaagcgcg gcgggcactc gggatc
//
LOCUS       DATMGX010001009         3226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_88437, whole genome shotgun sequence.
ACCESSION   DATMGX010001009 DATMGX010000000
VERSION     DATMGX010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88437"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VNR65_16525"
     CDS             <1..1069
                     /locus_tag="VNR65_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20322.1"
                     /translation="LKRRKRKAKIGAGRSVTVEDYVREQPPVYNGPKRPDEIMALLPK
                     PPKPPVEAHAPIPVLADFLREADAVYGFRPERVSEDDFMISYAAEAIRLGLARDQVVR
                     LYALETGGMGTQDLQSGFNPKTGRAASTALGYAQLLAANSIEQVRKEGPEFAARLDRL
                     AEVPSVSSSTAQALRYKAACVRRMIADARKVPESWPAHEAYAKTANGLGMHALNLDGN
                     VGPWMQVVKLRSIMEYASKKGFHHLNGAQLELMNLAGPGSGLEMLQSPGRDVPTSNFF
                     ERGGYGRNPVVHDKTGAQLLARLDEIMDRNVQKPGAQQFAKIFDGVARRMAAGGQIQT
                     SAEAASPSFNPFGSLNIFGPR"
     gene            complement(1761..1952)
                     /locus_tag="VNR65_16530"
     CDS             complement(1761..1952)
                     /locus_tag="VNR65_16530"
                     /inference="COORDINATES: protein motif:HMM:NF019511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HWJ20323.1"
                     /translation="MQKPTEADIERRRREAAKRTLEEAQLRKREDDAGALPKELGGRK
                     GPEPIRYGDWEKKGIASDF"
     gene            complement(2014..3117)
                     /gene="ychF"
                     /locus_tag="VNR65_16535"
     CDS             complement(2014..3117)
                     /gene="ychF"
                     /locus_tag="VNR65_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWJ20324.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQTAAAQAANYPFCTIEPNVGE
                     VSVPDDRLDKIAKIAGSKATIPTRLTFVDIAGLVRGASKGEGLGNQFLANIREVDAIA
                     YVLRCFEDDDVTHVEGRVDPISDAETVETELMLADLESLERRRLALEKKAKGQDKEAK
                     AALALVDTALRFVSAGQPARMALTEIPESEHRAFRQLQLLTAKPALYVCNVDEASAEN
                     GNAYSRLVEEHFSGAESENVVISAKIEAELAHLDEEERKLFLSELGLEEPGLNRLIAA
                     GYHLLGLITYFTAGPQETRAWTITKGTTAPGAAGVIHSDFEKGFIRAETIAYRDFVTL
                     GGEAGAKEAGKMRLEGKDYTVHDGDVMHFRFAT"
BASE COUNT          635 a          971 c          954 g          666 t
ORIGIN      
        1 attgaagcga cggaaacgga aggcaaagat cggggctggg aggtctgtga ctgtcgagga
       61 ttacgtcagg gagcagccgc ccgtctataa cggtccgaag cggccggacg agatcatggc
      121 gctccttccg aagccgccga agcctcccgt tgaagcccat gcgccgatcc ccgttcttgc
      181 cgacttcctc cgcgaggcgg atgcagtcta cggcttccgg ccagagcggg tcagcgaaga
      241 cgatttcatg atctcttacg cggccgaggc catcaggctc ggtcttgcgc gcgatcaagt
      301 cgtgcgcctt tatgcgctgg agacgggagg catgggtacc caggatctcc agtcgggctt
      361 taacccgaag accgggcgcg ctgcgtcgac ggcgctgggc tatgcgcagc tgctcgcggc
      421 aaactcgatc gaacaagtcc gtaaagaagg ccccgagttc gccgcgcgcc tggatcgcct
      481 cgccgaggta ccgagcgttt cgtccagcac ggctcaggcg ctgaggtata aggcagcgtg
      541 cgttcggcga atgatcgctg acgcccgaaa agttcccgaa agctggcctg cccatgaggc
      601 ctacgcaaaa acagccaacg ggctggggat gcacgcgctc aacctcgatg gcaatgtcgg
      661 cccttggatg caggtggtca agctgcgcag cattatggag tatgcctcga aaaagggatt
      721 ccatcatttg aacggggctc agcttgagct gatgaacctg gcggggccgg ggagcgggct
      781 tgaaatgttg cagtcgcccg gccgcgacgt tccgacctcg aactttttcg agcgcggcgg
      841 ctacggacgc aacccggtgg tgcacgataa gacgggggct cagctgcttg cccggctcga
      901 tgagatcatg gaccggaacg tccagaagcc cggcgctcag caatttgcaa agatcttcga
      961 cggcgttgcc cggcgcatgg cggccggcgg tcagattcag acatcggcgg aggcggcctc
     1021 gccctccttc aatcctttcg gctccctcaa tatttttggc cccagatagg taagatttac
     1081 ggcatccaaa tagttaatgt gcctgcgaca agtccatgat cccgcttagg tgaataccaa
     1141 gcggtcgatg ttaggtttgc agtgtggagt ttgagctacg ggatgaattc tttcgcggcg
     1201 catagctctg caagcagcag aatcatcata cacgttcatc agcatcagcc ggccgcccga
     1261 accgaaactt acaagcactt cagaggataa gatggctaat cgcgcgcgct ggacgtatgg
     1321 tgcgtctttc cttgttttgt ctttgatcat cagcccggtt gcttttgcag ccgccgaaaa
     1381 cgctgaccaa gagaagcctt cgcaagcaga gccggactcg aaatcctatc ttccgccgtg
     1441 gatgcagggc caaggcggca gcgagaaagc aagtgcgccg gccaatgccg ctgcgccagc
     1501 taacaacacc acgccggaaa gcaaatctgc ggcggccgat aatgcgccga caaagagaag
     1561 gttgcgagcc ggaagccagg cacagcgatc ccaccggcgc agggcgccaa ctgatgactt
     1621 tactggcggc tttgccgggt tttttggcgg ccgctgatgg gtgatttgag ccacgaaagt
     1681 cgggagttaa cagcgcctcc gacagtttca tagctcaaga tgttggctta ggaccgcagc
     1741 cgacgcaatt gcttggcttg ctaaaaatcg gacgcgatcc cttttttctc ccaatcgccg
     1801 tagcgaatgg gttccgggcc tttccgtccg ccaagttctt tgggcagcgc accagcatca
     1861 tcctcccgtt tacgaagctg agcctcttcc agcgtgcgct tggcggcttc tctacgacga
     1921 cgctcgatat cggcttcggt cggcttttgc attctcaacc tcgattgcgc tcgctaccct
     1981 gagacggttc ggcgcgcgct ttttacttgg agatcaggtc gcaaaacgga agtgcatgac
     2041 atcgccgtca tgcaccgtgt aatccttgcc ctcaaggcgc atcttgcccg cttctttcgc
     2101 cccggcctcg ccaccaagtg tcacgaaatc gcgataagcg atcgtttccg cccgaatgaa
     2161 gcctttttca aagtcggaat ggataacacc tgcggccccc ggcgcagtgg tgcccttcgt
     2221 gatggtccag gcgcgcgtct cctgcgggcc agccgtaaag tatgtgatca gcccaaggag
     2281 gtggtaaccc gctgcaataa ggcggttgag cccgggctct tccaacccca gctcgctgag
     2341 aaacagcttg cgttcctctt cgtccaaatg cgcaagctcg gcctcgattt tcgccgatat
     2401 gacgacgttc tcggattcgg cgccgctgaa gtgctcctcg accagtcttg aatacgcgtt
     2461 gccgttctcg gcgctggcct cgtccacgtt gcagacatag agcgcgggtt tcgctgtcag
     2521 caattgcaat tgcctgaacg cgcgatgctc gctttcgggg atctcggtga gagccatgcg
     2581 cgcgggctgc ccggctgaga caaatctcag agcggtgtcg accaaagcaa gggccgcctt
     2641 cgcctccttg tcctgaccct tcgccttctt ttccaaggct agcctgcgcc gctcgaggct
     2701 ctcgagatcc gcaagcatga gctcggtctc gaccgtttcg gcgtcggaga taggatcgac
     2761 acgtccctca acatgggtca cgtcgtcatc ctcgaagcag cgaagcacat aggcgatcgc
     2821 gtcgacctcg cggatattgg ccaggaactg gttgccaagc ccctcgcctt tagaagcgcc
     2881 gcgcacgaga ccggcgatat caacgaaggt cagccgggtt gggatggttg ccttggaccc
     2941 cgcaatcttg gcgatcttat cgagccggtc gtccgggacg gacacctcgc ccacattcgg
     3001 ctcgatcgtg cagaaaggat agtttgccgc ctgcgccgcc gcggtctgcg tcagcgcatt
     3061 gaaaagggtg gatttaccga cattcggcaa tccgacgatg ccacatttga agcccatagg
     3121 tgttccgggg agtcctttgc ctgctatttt gtcccgccgc gaacgcgctt ccggaaccac
     3181 cgcttgaaga tattgtcctc gccctcatga gatgccgccg atggcg
//
LOCUS       DATMGX010001010         7875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_239897, whole genome shotgun sequence.
ACCESSION   DATMGX010001010 DATMGX010000000
VERSION     DATMGX010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239897"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(492..656)
                     /locus_tag="VNR65_16540"
     CDS             complement(492..656)
                     /locus_tag="VNR65_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20325.1"
                     /translation="MTTMFVRHTVSNYEVWRKAYDDFAPAFLNSDELKNAMQGGGVVG
                     APTIWFTDKK"
     gene            1128..1451
                     /locus_tag="VNR65_16545"
     CDS             1128..1451
                     /locus_tag="VNR65_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20326.1"
                     /translation="MRRAIEEKVMFGVDAVDAKLNQSLGVTLKGLAPILKRGGDEAEK
                     KFMECCWCLWRANGSELLEPFIEAAFNTLPKKSRALLFERMLTIVYVAQDSVVQNSTD
                     IRSRG"
     gene            1604..4177
                     /locus_tag="VNR65_16550"
     CDS             1604..4177
                     /locus_tag="VNR65_16550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWJ20327.1"
                     /translation="MARLSATGGRASKAKAGRASQAKGRKTARTSPNDKRRRNAERET
                     EVAGLKRELKEALARQTATSEVLRVISQSPTDTQPVFEAIVLTAVRLLHCDMSIVMLC
                     EPPAYWVAAAATPEGLFDELTLIKQPIDPRANFPSRTIVEKRALYLPDWSLIDLPEHE
                     RHIHETVGVNSALYLPLMREGECIGLLTLGGKQANMFGESEIALAESFRDQALIAIEN
                     TRLFNETREALEQQKASADILSVISNSVADPKPVFDKILRSIEHLFDGQERFIFQVGE
                     DGLLHIAAAHGPNVERLRALFPIPVEGTANEQAIRERRLVRYADVLNDPGVPSGLRET
                     ARRFGQNYSMVVAPMLWENRAIGSVFVGRTSMEAFSDKECELLRTFADQAVIAIQNAR
                     MFDEVQAKTRDLTESLQQQTATADVLKVISRSAFDLQTVFNTLIDSAVALSGALSGTI
                     CLRDGDGYRYAATSFDMQSDFAKWLFEHPPTRGRASAAGRVLVSGKVEEVPDILEDPD
                     YTLPVVSLNRTRSVIGVPLLGKDRLEGVLALTRAEPGHFTQRQIELVQTFADQAVIAI
                     ENVRLFDEVQARTKELAASLDDLRTAQDRLVQTEKLASLGQLTAGIAHEIKNPLNFVN
                     NFSALSAELIDELDETLAPAPLDQKMRGDVGELTQMLKSNLEKVVQHGKRADSIVKNM
                     LLHSREGSGEHRSADVNALVEESLNLAYHGARAEKPGFNVILKRDLDPDAGAVELYPQ
                     EITRALLNLISNGFYAATQRKSVEGETFEPVLIATTKNRGKTVEIRIRDNGTGIPDEV
                     KEKMFNPFFTTKPTGEGTGLGLSMSHDIIVKQHGGSIDVETAPGFFTEFKIVLPRTSQ
                     R"
     gene            4558..5883
                     /locus_tag="VNR65_16555"
     CDS             4558..5883
                     /locus_tag="VNR65_16555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ20328.1"
                     /translation="MQDIEKRVIGKVMWRLIPFLIVCYFVAYLDRVNVGFAKLHMNQA
                     LGLSEAAFGLGAGLFFVGYFLFEVPSNIFLERVGARIWIARIMITWGIVSAAFAFIPS
                     ISAATGIPTTGVFYSLRLLLGACEAGFFPGIIFYLTLWFPAVYRAQVISLFMLAIPIS
                     SIIGSPISGMLLGVTGLGLQGWQWLFILEALPSVLVGIGVLIYLTDFPRQARWLQQNE
                     IAWLENVQATEKRNKEKVEQLSLVQALTDPRILLCALVYFCLNAASYGVAFFLPTIIK
                     GFGVTDTQTGLLAALPFVFGAVGMVLLGHHSDRTMERKGHVAAALLMATVGIGLAGLV
                     SNPVIVMALLCFAQIGVSAVPPMFWPLPASFLTGASAAAGIAAINSLGNLSGFAGPYA
                     MGYLKDLTGSFTAGLLLLAGCALVGAVVVVLLRIDARREQVSGEIAMAH"
     gene            6145..7443
                     /locus_tag="VNR65_16560"
     CDS             6145..7443
                     /locus_tag="VNR65_16560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ20329.1"
                     /translation="MSLVEPSGAIEVAPIEDSSLLAFYRDMNLPERRTFWACASGWAL
                     DGMDFMIYPLVIGTIIALWKVDPGTAGLAGTVTLLASAIGGWLGGYLADRIGRVKTLQ
                     FTILWFSFFSLLCAFVQNFDQLLIARALLGLGFGGEWAAGAVLMGEAIRPQYRGRAVG
                     SVQSGWAVGWGLAVLSQAILFSYLPPETAWRWMFAIGALPALLVFFLRRYVEEPVIAA
                     ETRAKQVASGDRPALWEIFSGPILKTTILASLMATGCQGGYYAITFWVPRFLTTERKL
                     SIVSSTGYLAALIIGSFIGYLVGAWLADRIGRRNLFLTFSIGAIAVILLYTQLPLTNE
                     VLWLLGFPLGFFASGYFSGVGAFLTELYPTRLRGSGQGFCYNFGRGIGALFPFLVGWL
                     SISTSLGNAIAIFAVLAYGVFFIAAFALPETRGRILHADA"
BASE COUNT         1548 a         2243 c         2356 g         1728 t
ORIGIN      
        1 gggaaccggg agatctcatg gtcggccagc gtcagcatgc gtgctgcact ggtccgcgtc
       61 gggaaggcga ggagccgtag ccgatgatgt acgatcatga gaagtctgac cctgccatag
      121 tagctgtgaa gccgacgagc aaagccgggc aaccggctgc ggagttggtg gagccaaggg
      181 cgggggccga ggggaatgcg agtcagcaaa gcacgggccg ggctcagtac cggataatcg
      241 tgtcacaggc gctgaagcgc atacggcacg ttgcgaggtt attgcgaagt gccgtttaca
      301 cacccgaggt gggagccgta tgcccgaatt gggcccgtac ggatctgtgc ggggggcgcg
      361 aggtaactcg cgtccctacc gcgaaagtgg acgcgcgatg gccaaaatca cgtgactgcc
      421 cctgaacgga catcgggcct agctagtggg acggcggtcc gggttcgatt cgtgacgcgg
      481 gtagctacgc cctatttttt gtcggtgaac cagatggtag gagcgcccac cactccaccg
      541 ccctgcatgg catttttcaa ctcatcgctg ttgaggaagg ctggcgcaaa atcgtcgtag
      601 gcttttcgcc agacttcata gtttgaaacg gtgtgtctaa caaacatggt tgtcatttgt
      661 tttgccctaa ttgatcgttt tgaacggtct caatacgatc atcaattccc acgtacggca
      721 agtttcggcg cgaggctaaa aactgacgcg ctgacgatga tgtccgttgt tcggagtaga
      781 ccggaagtgg cgtttcgggg ccatgaggac cgtttttgac ccccaacggt catcggcggt
      841 caccaaatat ttacgcgaaa gcaccattga catggatcaa atgcgtgcag ccgtttttga
      901 cgtccaaatg ttggtctgat gaatggagga ccacagcaag gcgataaatg cgcaggtcgc
      961 agaactggaa tggcgacatc ataagctgga gaggaagatt gccgacacgt tagctcatag
     1021 ttccactgac gatctgacga atcgcggagc tgaggcgcca aaaatacagc taaaagatga
     1081 gatagaacgc ttgcgttacg aacacggctg actcgagttt gataacaatg cgtcgcgcca
     1141 tcgaggagaa ggtcatgttc ggggttgatg ctgtagacgc caaacttaat caatcgttgg
     1201 gagtgactct gaaagggctt gcacctattt tgaagcgcgg cggcgacgag gcggaaaaga
     1261 agttcatgga gtgctgctgg tgcttgtgga gggcgaatgg aagtgagctg ctggagcctt
     1321 tcatcgaagc cgcctttaat acgttgccta agaaatcgcg cgctttgctg ttcgagcgaa
     1381 tgttgacaat cgtctatgtg gcgcaagaca gtgtcgttca gaactccact gatatccgtt
     1441 cccgggggta aagcagacct cgcagtcgga cagcccgact tccgagttag acccatttcc
     1501 gacattgcac ggatggtcgg cgaccccata cctgtgcaga atcaacccct ttcggtctta
     1561 tggccgagac cgataaggtg ctgttcggaa cataggggcg gatatggcgc ggctttcagc
     1621 gacgggcggc agggcgagca aggcgaaagc cggcagggca agtcaggcaa aaggccgcaa
     1681 aacagccaga acctcgccaa acgataagcg acgtcggaac gcagaacgtg aaacagaggt
     1741 cgcggggcta aaacgtgagc taaaggaggc gctggcccgg cagaccgcga cctccgaggt
     1801 cctgcgcgtc atcagtcaat ctccgaccga cacgcagccg gtgttcgaag ctatcgtcct
     1861 taccgccgtg cgattgctcc actgtgacat gtcgatcgtg atgctttgcg aaccgcccgc
     1921 ctattgggtt gcggcagcag ccacaccgga aggcctgttt gacgagctta cattgataaa
     1981 acagccgatc gatcccaggg ctaactttcc ctcgcgtacc attgtcgaga agcgggcgct
     2041 ttacctgccg gactggtcgc tcatcgacct gcccgaacac gagcgccaca tccacgagac
     2101 ggttggcgta aactcggcac tgtatttgcc gctgatgcgc gagggcgaat gcatcggcct
     2161 gctcacgctg ggtggaaaac aggcaaatat gtttggtgag agcgaaattg cgctggccga
     2221 atccttccgt gaccaggccc tgatcgcgat cgaaaacaca cggctgttca atgagactag
     2281 ggaagcgctg gagcagcaga aagcttctgc tgatatcctg agcgtgatca gcaattcggt
     2341 cgccgacccc aagccggtat tcgacaagat cttgcgcagc atcgagcatc tgttcgacgg
     2401 acaggagcgt ttcatatttc aggtgggcga agatgggttg ctgcatatcg ccgctgcaca
     2461 tggccccaat gtcgagcggt tgcgcgcgct gttcccgatt ccggtggaag ggaccgcaaa
     2521 tgagcaagca atccgcgagc gtcgtcttgt gaggtacgcg gacgtattaa acgaccccgg
     2581 cgtaccttcc ggccttcgcg agaccgctcg ccgtttcgga cagaactatt cgatggtggt
     2641 cgcgcctatg ctctgggaaa accgcgccat tggttcagtc ttcgtagggc gcacttccat
     2701 ggaggcgttc agcgacaagg aatgcgagct gctgcgcact ttcgccgatc aggccgtaat
     2761 cgcgatccag aatgcccgca tgtttgacga ggtgcaggcc aagacgcgcg atctgacgga
     2821 atctttgcag cagcagaccg cgaccgccga cgtgctcaag gtcatcagcc gttcggcctt
     2881 cgacctgcaa acggtgttca atacgctgat cgactcggcg gtcgccctca gtggcgccct
     2941 cagcggcacg atctgcctgc gcgatggcga cggttatcgc tatgccgcga cctccttcga
     3001 catgcagagt gacttcgcca aatggctctt cgagcatccg ccgacacgcg gccgcgcctc
     3061 ggcggcgggc cgcgtgctgg tatccggcaa ggtcgaagaa gttcccgaca tcctcgagga
     3121 ccccgactac accctgccgg tcgtgtcgct gaacaggacg cgctcggtga tcggcgtgcc
     3181 gctgcttgga aaggacaggc tcgagggagt gctggcgctg acgcgcgccg aacccggtca
     3241 ttttacccag cgccagatcg aattggtgca gaccttcgcc gaccaggcgg tgattgccat
     3301 cgagaacgtg cggctgtttg acgaagtgca agcgcgaacc aaggagctgg cggcttcgct
     3361 cgacgacctt cgcaccgcgc aggaccggct ggtgcagacc gaaaaactcg cctcgcttgg
     3421 ccagctcaca gccggtatcg cgcacgagat caagaacccg ctcaacttcg tcaacaattt
     3481 ctctgctctt tcagcggaac tgatcgacga actggacgag actctggctc cagcgccact
     3541 cgatcagaag atgcgcggag acgtcggcga actgacccaa atgttgaagt ccaaccttga
     3601 aaaggttgtg cagcacggta agcgcgccga ctccatcgtt aagaacatgc tgctgcactc
     3661 gcgcgagggc tcgggtgaac accggtccgc cgatgtcaac gccctggtcg aggaaagtct
     3721 caatctcgcc tatcacggcg cgcgggccga gaagcccggc tttaatgtca tactgaagcg
     3781 cgacctggat cccgatgcgg gcgcagttga actttacccg caggaaatca cccgcgcttt
     3841 gctcaatttg atttccaacg gtttctacgc cgctacccag cgcaaatcgg tagagggaga
     3901 aacctttgaa cccgtgctga tcgccaccac gaaaaatcgc ggcaagaccg tcgaaattcg
     3961 cattcgcgat aacgggaccg gcatccccga tgaggtcaaa gagaagatgt tcaacccgtt
     4021 cttcacgacc aagcccacgg gcgaaggtac cgggctaggc ctttcgatga gccacgacat
     4081 catcgtcaaa caacacggcg gcagcattga tgtcgagacg gcgcccgggt ttttcactga
     4141 gttcaagatc gtgttgccgc ggactagcca aaggtaatcc aacacgctca tttcaacggc
     4201 tgaccggcat ctattttatt gcggtgcatg agtctcttgc tggcactgac cggaccaacg
     4261 gcgccaacct gatgatgtcc gtttctcggg gcagaccgga agtggctcgg acatcggcaa
     4321 acggacgcga ttggacccga gcccgacatc gagcgcccag cccgaagtat ttcacccctt
     4381 catttaatgg tgcgcgctgt ttgggttcga actcgcaatt ttcgcgatcg accgcagtgc
     4441 accactgtgt tcttaacaca aatgccattt gcgctaatcg ttcgcgaccg tcatagtttg
     4501 acaaaatccg cccgcatttc tgaacgggcc tgcaaacaca tgacgggaga ttgcgccatg
     4561 caagacatag agaaacgtgt catcggcaag gttatgtggc ggctaattcc gttcctgatc
     4621 gtttgctatt ttgtggccta tctcgatcgc gttaatgtcg gcttcgccaa gttgcacatg
     4681 aaccaagcgc ttggtttgag tgaagcagcc ttcggactgg gcgcgggatt attcttcgtc
     4741 gggtacttcc tgtttgaagt accctccaac atttttttgg aaagagtcgg cgcgcgaatt
     4801 tggatcgccc gcattatgat tacctggggg atcgtttctg cggcgttcgc attcatccca
     4861 tcgatatcgg cagcgaccgg aattccgacg acgggtgttt tctattctct ccgtttgctg
     4921 ctcggcgcct gcgaggcggg cttctttcct ggcatcatct tctatttgac tctgtggttc
     4981 ccagcggttt accgcgccca ggtcatcagc ttgttcatgt tggcgatccc gatttcgtcg
     5041 atcatcggat cgcctatctc cgggatgctg ctcggcgtga cgggtttggg gttgcaaggc
     5101 tggcagtggc tgttcattct cgaggcgttg ccgtcggttc tggtgggcat cggcgttctg
     5161 atctacctaa ccgatttccc gcgccaggcc cgctggctgc aacagaatga gatcgcgtgg
     5221 ctggagaacg tgcaggccac cgagaagcga aacaaggaga aggtcgaaca gctatccttg
     5281 gtgcaggcgc tcacggatcc gcgcatctta ttgtgcgctc tggtgtattt ttgcctgaat
     5341 gctgcgagtt acggcgtggc gttcttcctg ccgaccatca tcaagggttt tggcgtcacc
     5401 gatacccaga ccggattgct cgcggcgctg ccctttgttt tcggggccgt gggaatggtg
     5461 ctgctcggtc atcattccga tcgaaccatg gagcgaaaag gtcacgtcgc cgcagcactt
     5521 ttgatggcca cggtcgggat cggattggca ggtctcgttt ccaaccccgt catagttatg
     5581 gcgttgcttt gttttgcaca gatcggcgtc tccgcagtac ccccgatgtt ttggccgcta
     5641 ccggcaagtt ttctgacggg agcatcggcc gccgccggca tcgccgcaat taactcgctt
     5701 ggaaatcttt ccggtttcgc cggtccttat gcgatgggct acctgaagga tttgaccggc
     5761 agctttaccg cgggccttct attgctcgcg ggttgcgcgc tggtgggcgc cgtcgttgtc
     5821 gtgctgctgc ggatcgatgc taggcgcgag caagtgtcgg gtgaaatcgc gatggcgcac
     5881 tagacaaagc gacgctgttt gctgcagtgc atgagtctct tcctggcacc aaccggacat
     5941 ggcgagatgt ccgacttgag tccgctttcg ggagtatagc agacgttatt tgctcccagc
     6001 gagtatttcg ccttttgacc caactcggac atttgactaa tgacagcatc agggatcagc
     6061 gtaggatttg agcaccccac gcaaatcggc cgccgcgggg gctaagcaaa ggccgtcaga
     6121 aatcctccgg gcggagcttc caccatgagc ctagtcgagc catcgggagc gatcgaagtc
     6181 gctccgatcg aagattccag ccttctcgcg ttttatcgcg acatgaatct gccggagcgg
     6241 cggacgttct gggcttgcgc gtcaggctgg gcgctcgacg gcatggactt catgatctat
     6301 ccgctggtca tcggcaccat catcgcgctg tggaaggtcg atcccggcac tgccggcctc
     6361 gccggcaccg tgacgttgct cgcttccgcc atcggcggct ggctcggcgg ttatctggcc
     6421 gaccgcatcg gccgcgtgaa aacattacag ttcacgatcc tctggttttc attcttctcg
     6481 ctgctctgcg cctttgtgca gaatttcgat cagctgctga tcgcgcgcgc gctgctcggt
     6541 ctcggtttcg gcggggaatg ggccgcgggc gcggtattga tgggcgaagc gattcggccg
     6601 caataccgcg gacgcgcggt cggttcggta cagtcgggat gggcagtcgg ttgggggctt
     6661 gcggtgctgt cgcaggccat tctgttctct tatttgccgc cggagacggc atggcgctgg
     6721 atgttcgcga tcggcgcgct gcccgcgcta ttggtgttct tcctgcgccg ctatgtcgag
     6781 gagccggtga tcgccgctga gacgcgtgcc aagcaggtgg cctcgggcga ccggcccgcg
     6841 ctatgggaga ttttctcggg tccgattctc aagaccacta tcctggcgtc gctgatggcg
     6901 acgggatgcc agggcggcta ttatgccatc accttttggg taccgcgctt tctcaccacc
     6961 gaacgaaagc tttcgatcgt cagctccacg ggctacctcg cggcgctgat catcggctcg
     7021 tttatcggct atctggtcgg cgcgtggctt gcggaccgta tcgggcggcg caacctgttc
     7081 ctgaccttct cgatcggtgc gatcgccgtg atcctgctct acacacaatt gccgttaacc
     7141 aacgaggtgc tgtggctgct cggcttcccg ctcggcttct tcgcttcggg ctacttctcc
     7201 ggcgtgggcg cgttcctcac cgaactctat ccgacccggt tgcgcggctc gggccagggc
     7261 ttctgctaca atttcggccg cggcatcggc gcactgttcc cgttcctggt cggctggctc
     7321 tcgatctcga cgtcgctcgg caacgccatc gcgatcttcg ccgtcttggc ttacggcgtg
     7381 ttcttcatcg cggcatttgc gctgccggag acgcgcggcc gaattctgca cgcggatgcg
     7441 tagatgggtt tgtttcttgg gaacggtacc tgaagcctcg ctccgtcgtt ccttctcccc
     7501 ttgcggggga gggagaagaa actgactgtt caatcaggct tgcatcgcgg aattcaggac
     7561 cgtcctgccg ccgacgatcc aaggtgaccc cactcatctt acccatttgg ccagtgtctg
     7621 cgatgcttga gcctggcttt ggccacgcaa cggtcattgc ggtgcagcca ccgcacgtcc
     7681 gttgtcggag gcgaagcgga aaacatgtgc tcggcgagag tatttcgcct tttgacccaa
     7741 ttcggacata agacgcttgg accctcgcta acattcgcct aggcttaagt ccgtcgtgga
     7801 atctgtgacg atttgaagcg acgcgattga accgaactgt cctgtccact catgcgctcc
     7861 ggaatgcccg acgga
//
LOCUS       DATMGX010001011         2898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_120657, whole genome shotgun sequence.
ACCESSION   DATMGX010001011 DATMGX010000000
VERSION     DATMGX010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120657"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..764
                     /locus_tag="VNR65_16565"
     CDS             462..764
                     /locus_tag="VNR65_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20330.1"
                     /translation="MDERDLYDERQETRKTTLNCPHCHESAEYELGWFVRTKKRQLPP
                     RADERDRAKFAKARSYMVRRDDLVACKNIRCRKRFEVSGVQSVAFLDEQVKPETRQ"
     gene            838..2031
                     /locus_tag="VNR65_16570"
     CDS             838..2031
                     /locus_tag="VNR65_16570"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HWJ20331.1"
                     /translation="MNWNPQRAAPPPPRKDKVTARVEPVVVRLEKFWQRVTDGMKLNE
                     LWKQFQADARFSYRLYSQDVDSTRTAGTPKRKHVFNIARQFFWAMLEKLSPARRVLLL
                     IALVLILFPSGHAEWQSGNGGLSINLDTHFWGGLLMFALLLLEVADRVVMKRDLQIAK
                     EIQAWLLPASPPAVPGLEIAFATRPANTVAGDYYDVFPRPSSSSSGETFLMAVADVAG
                     KSIPAAMLMATFQASLKTLCTWPGSLVELLARMNAYACSNSQNGRRFTTAFIAEYDPV
                     SRTLTYVNAGHNNPILRRQSGSIERLQVGGVPLGILENAPYESGSVTLQSGDWLVIFT
                     DGVVEAENQRQEEYGEARLFAVLQAGVTYAPAALLNYIMGDLDRFVANAPQHDDVTLM
                     LLRAT"
     gene            complement(2047..2442)
                     /locus_tag="VNR65_16575"
     CDS             complement(2047..2442)
                     /locus_tag="VNR65_16575"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HWJ20332.1"
                     /translation="MSVNAKNGLIHLASQHTVEETIQRFEALLSERGVTIFARVDHSG
                     EAAKVGLAMRPTKLLIFGSPKGGTPLMQAAPTLAIDLPLKALFWQDAEGKVWLTYNDP
                     SYLQQRHNFPAELIPNISGVSALFGKAVE"
     gene            2560..>2898
                     /locus_tag="VNR65_16580"
     CDS             2560..>2898
                     /locus_tag="VNR65_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20333.1"
                     /translation="MPSSRWDAVIFDYGRVLSLAPSSAELQEFAALVGVTEPPFFEIY
                     SATRHDYDAGRADFRQHWQSFADAAGVELGPAQVERIAGMETLMWLRVNPEALALARE
                     IKAHGVRTAIL"
BASE COUNT          565 a          899 c          843 g          591 t
ORIGIN      
        1 cgggtagtgc ggacattcag gtagcgacca agctctttaa tccttgtcat catgagtgtc
       61 acatgggcct gcggcgcacc catagaaatg agaattcacc acggagacac ggagatcact
      121 aagaaaactc taaagaaact agaactccgt gccctccgtg tctccgtggt gaatgatttt
      181 cgacggagtg ggcgaaccgg ggtccccaaa cgagcgccgt ttttgcgctt gttggggtaa
      241 agttcagccc gcgagggatc tgctttccga ctcttcagcg gcggaatcgg cctccttcct
      301 gcacctcgaa agcccgctgc acccgttcca gcccgataca ctaattctgc aaccgctctc
      361 ggcaaagccg tagctcgtat cacatcctcg aagcggcgac tatgttctaa tactatggcg
      421 ggattgagcc acctctctga tggcccacac ggaggaagct gatggatgag cgcgacctgt
      481 atgacgagcg gcaagagacc agaaaaacga ccttaaactg ccctcattgc catgagtccg
      541 ccgaatacga actgggttgg ttcgttcgaa ccaagaagcg ccaactgcct ccgcgggctg
      601 acgagcgtga ccgtgcaaag tttgccaaag cccgttccta catggtccgg cgcgacgacc
      661 ttgtcgcctg caagaacatt cgctgccgca agcgctttga ggtctcgggc gtccagtcgg
      721 tggcctttct cgatgaacag gtaaaaccag agacccggca gtgactcggt tgccgccggc
      781 cgaatcctcg tggttcttcg ccccagacct caccttctat aatcctgttt ccacggaatg
      841 aactggaacc ctcaacgcgc agctccgccg ccaccgcgta aggacaaagt tacggcgcgc
      901 gtcgaacccg tcgtggtccg cctggagaaa ttctggcagc gcgtcaccga cggcatgaag
      961 ctcaacgagc tttggaagca attccaggcc gacgctcgtt tcagctatcg gctttactcc
     1021 caggacgtag actccactcg caccgcggga acacccaagc gcaagcatgt cttcaatatc
     1081 gcgaggcagt ttttctgggc gatgctggag aagttgtcgc cggcgcgccg cgtcctgctg
     1141 ttgatcgcgc tcgtcctgat tctctttccc agtggccacg cggaatggca aagcggcaat
     1201 ggtggcttga gtatcaatct cgatacccat ttttggggcg gattgctgat gttcgcgctc
     1261 ttgctgctgg aagtcgccga ccgcgtggtg atgaagcgcg atctccagat cgcgaaggaa
     1321 atccaggcgt ggttacttcc ggcgagtcct cccgccgtgc ccggcctcga gatcgcgttt
     1381 gctacccgcc cggcgaatac cgtggccggc gactactacg atgtcttccc ccgtccaagt
     1441 tcaagttcat ccggcgagac gtttctaatg gcggtggctg acgttgccgg caagagcatt
     1501 ccggccgcca tgctgatggc gacctttcaa gccagcctga agacgctttg cacatggccg
     1561 ggctcgctgg tcgaactgtt ggcccgcatg aacgcctatg cctgcagcaa cagccagaac
     1621 ggccgccgct tcaccaccgc gttcatcgcc gagtacgatc cggtgtcccg gactttgacc
     1681 tatgtcaatg ccggccataa caacccgatc ctgaggcgtc agtccggcag catcgagcgc
     1741 ttgcaggtcg gaggcgtgcc gctcggcatt ctggagaatg ctccttacga gtcaggctcg
     1801 gtgacgctgc aaagcggcga ttggctggtg atcttcaccg acggcgtggt ggaagccgag
     1861 aaccagcggc aagaggagta tggcgaggct cgcctgttcg cggtgctgca agccggagtg
     1921 acgtatgccc ctgccgcgct gctgaattac atcatgggcg acctcgaccg cttcgtcgcc
     1981 aacgccccgc agcacgatga cgttacgctc atgctgctgc gggcgacgta acttctcgcg
     2041 tgcgggctac tccacagcct tcccgaacaa cgcactcaca cccgaaatgt tgggaattaa
     2101 ctccgcggga aaattgtggc gctgctgcaa atacgaagga tcgttgtagg tgagccaaac
     2161 cttcccctcc gcgtcctgcc agaacaacgc tttcagcggc aggtcaatcg caagagtggg
     2221 agcggcctgc atcaacgggg ttccgccctt cggactgcca aaaatgagca gcttggtagg
     2281 ccgcatggcg agtccaacct tcgcggcctc gccgctgtgg tccacgcgcg cgaagatggt
     2341 gacgccacgc tcactgagca gcgcttcgaa gcgctgtatg gtttcttcaa ccgtgtgctg
     2401 actcgccagg tgaatgagcc cgttctttgc gttcacactc atattgactc ccgctgcttc
     2461 gttgcgcttg ctttcagcgc ggcggaatca ttatgaaggc ccggatcggc gccggcaaac
     2521 ctcgttcgaa gaactctctc ccgtgtaaga atggagccga tgccatcgag ccgctgggat
     2581 gctgtcatct tcgactacgg gcgggtgctg agcctcgcgc cttcgagcgc cgagttgcag
     2641 gaattcgcgg cgctggtggg cgtcaccgag ccgcccttct tcgagattta ctctgccact
     2701 cgccatgact acgacgccgg ccgcgccgac ttcaggcagc actggcagtc tttcgctgat
     2761 gccgccggcg tggagctcgg gcccgcgcag gtggaacgta tcgccgggat ggagacgctc
     2821 atgtggctgc gtgtcaatcc cgaggcgctc gctctggcgc gcgagatcaa ggcacacgga
     2881 gtgcggaccg ccatcctc
//
LOCUS       DATMGX010001012         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_56470, whole genome shotgun sequence.
ACCESSION   DATMGX010001012 DATMGX010000000
VERSION     DATMGX010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56470"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VNR65_16585"
     CDS             <1..1073
                     /locus_tag="VNR65_16585"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWJ20334.1"
                     /translation="ASEVMAAFCLAEDMSDLAARLSNMVIGQNTVGHLIRVGALNSTG
                     GMTALLKDALQPNLVQTLEGTPAFIQGGPFANIAHGCNSVIATKSALKLADVVVTEAG
                     FGADLGAEKFLNIKCRQAGLKPDAAVIVATVRALKMHGGVAKSELGAENVEAVKRGLP
                     NLQRHIENMQKFGLRLVVAINHFGADTDAEFGAIAEACTKWGVDAIACTQWADGGKGA
                     EELARAVMALVRKPGELRLLYPDAMPLADKIRTVAKEIYRAGAVQFSTAAKRKLAHFE
                     KDGFGRLPVCMAKTQYSFTADPAVMGAPTGFTLPVRDARLSAGAGFVVALCGDIMTMP
                     GLPKVPAADHIGLDERGQIMGL"
     gene            complement(1192..1485)
                     /locus_tag="VNR65_16590"
     CDS             complement(1192..1485)
                     /locus_tag="VNR65_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20335.1"
                     /translation="MITLEDCIAFCGLTEEVVLVIAEHERIPEIVAAGLASSMLSVEN
                     GTEKIRNIIVDSIRQAQDYGDRRHVLRLLHVLHHFLKEHRQVIPSHRPWSACL"
BASE COUNT          427 a          584 c          653 g          397 t
ORIGIN      
        1 ccgcttcgga ggtgatggcg gcgttctgtc ttgctgaaga tatgagcgat cttgcggcgc
       61 gtctctccaa catggtgatc gggcaaaata cagttgggca cctgatccgc gtgggggcgc
      121 tgaacagcac cggcgggatg acggcgctgt tgaaggacgc cttgcagccg aaccttgtgc
      181 aaacgctgga gggcacgccc gccttcatcc agggcggccc gttcgccaat atcgcgcatg
      241 gctgcaattc ggtgatcgcg accaagtcgg cgcttaagct cgccgatgtg gtcgtgacgg
      301 aagcaggttt cggcgccgat ctcggggccg agaagttcct caacatcaaa tgccggcagg
      361 ctgggctgaa gcccgatgcc gcggtgatcg tcgccacggt gcgtgcgctc aagatgcatg
      421 gcggcgtcgc caaaagcgag cttggcgccg aaaacgtgga ggcggtgaaa cgcggcctgc
      481 cgaaccttca gcgccatatc gagaacatgc agaagttcgg gttgcgtctc gtggttgcga
      541 tcaatcattt cggagcggac accgacgctg aattcggcgc cattgccgag gcttgcacga
      601 aatggggggt ggacgctatc gcctgcaccc agtgggccga tggcggcaag ggagcagagg
      661 aactggcgcg cgcggtgatg gcgctggtgc gaaagccagg cgaactgcgc ctgctgtacc
      721 ctgatgccat gccgctcgcc gacaaaatcc gcaccgtggc aaaggagatc tatcgcgcag
      781 gcgcggtcca gttttcgacg gcggcgaaac ggaaactggc gcatttcgaa aaggacggct
      841 ttggccgcct gccggtttgc atggcgaaaa cgcaatatag ctttacggcc gaccctgccg
      901 tgatgggcgc tccgacgggg ttcacgctac ccgtgcgcga cgcgcggctt agcgcgggcg
      961 cgggctttgt ggtggcactc tgcggggaca tcatgacgat gccggggctg cccaaggttc
     1021 cggctgccga ccatatcggc ctcgatgaga ggggccaaat catggggttg tgagcctcga
     1081 ctgtgcggcc ttggcggccc cccatggcgc ggtgatgcaa ggggcggaaa gacacaacgc
     1141 acagcgcttt tgacgaagct gcctcctctg tggagggcag cgccgcccgg atcacaaaca
     1201 ggcgctccag ggacggtggg atgggatcac ttggcgatgc tccttgagga agtggtgcag
     1261 cacgtgcagg agcctcaaga cgtgcctgcg gtcgccataa tcctgggctt gccggatact
     1321 atcgacaata atgttccgga ttttttcggt gccgttctct acgctcaaca tggaactcgc
     1381 aaggccggcg gccacaatct cgggaatgcg ctcatgttcc gcgatgacaa gcaccacttc
     1441 ctcggtcaag ccgcagaacg caatgcaatc ttccagcgta atcataacct ttgatgttta
     1501 gcacaagctg tcgggattaa aaatgattaa tgtcaaagat gaaagatgat aaatgccgca
     1561 ccccgacaag cagaccgata ttgcgaattg ctggcgccgt gtaactgacg tggtcgcgcc
     1621 tacgggacat accctgaccg aagcgtccat cggaatggcg ctcgcgaccc caattttatg
     1681 aaggcgatcg gccgggaaaa tcattggcgg tccggagctg ttccagccgc gtgatcgatc
     1741 caagttcctg tcaccctcga tctcgcatct ttcggctgaa atgcatcgga tctcgatttc
     1801 tctcacctga aagcacgcgc catcgcgggc acactttcca caaattcgga gcgcgttgag
     1861 tgatatgttc tttatttgtt ctgccctcaa cggtagggtg gcgcgactga aacaatctgc
     1921 ggcaagccat ggcgacgttg attatcactg agaaaacaag ccaggccaaa gacctgaggg
     1981 cagcgctcgg cgatcgcttc gggcagatcc tgccggcaga aggacatctg ctgcggctgg
     2041 ccgaacctga cgaagtcgac g
//
LOCUS       DATMGX010001013         6457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_56541, whole genome shotgun sequence.
ACCESSION   DATMGX010001013 DATMGX010000000
VERSION     DATMGX010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56541"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VNR65_16595"
     CDS             complement(<1..516)
                     /locus_tag="VNR65_16595"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWJ20336.1"
                     /translation="MNIIPISFGLKTNPVAGDRDARQEQEALDAYSEIVTGVVESVAA
                     AMVGIQRKAKPGEPEGNPRMQGGAGSGIVITPDGYILTNNHVIQGARAIEVLFADGGT
                     APADVVGGDPDTDIALVRAHTARLAPVQLGDSDALRVGQLVVAMGNPFGLQASVTTGV
                     VSALRRTLRATT"
     gene            complement(1022..1669)
                     /locus_tag="VNR65_16600"
     CDS             complement(1022..1669)
                     /locus_tag="VNR65_16600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ20337.1"
                     /translation="MNQQEHPAHQFTNSWFNNLAKGVWDQLITNLNPTTILEIGSYEG
                     ASTCYLIDLLAQKKEIEVHCIDSWEGSAEHKSDGTNMSEVEARFLHNTKLSSELAAHN
                     VALAIHKGFSDYHLSKLFSDGKRNYFDFIYIDGSHYASDVICDAVLSFRLLRIGGVIA
                     FDDYLWAGHNIPFGVITDPLGCPKLGIDAFTNLYGNRIRLLSAPLYQIYVQKISD"
     gene            complement(2866..4677)
                     /locus_tag="VNR65_16605"
     CDS             complement(2866..4677)
                     /locus_tag="VNR65_16605"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWJ20338.1"
                     /translation="MAEMIPDRLPSGASAGEKRVFEVLQRLPDNCIVYYEPVVSNKYP
                     DFIVIIPEVGLLIIEVKGWYPGHIESGDLNQVRITSRGRTETQRHPIRQARDYMFGLM
                     DAAKAFNNSTPLLRSSGAHAGQLIFPLGNLVVLNNIKRDQLDSLGLTPLFPPSKVMTR
                     DEFEHLANIETEGLVPALKNFFDPWWDFAALNDTQISALRAIMHPEIVISKAPIGRDG
                     SRETLKTLDLRQERFERNIGEGHRILYGVAGSGKTVILIARARLLAAEANKEVLVLCY
                     NRPLAEHIQSIFDSQKNIICHNFHLWGSRHGVCFDKKEDVDYYGQRLLERLERGEGDA
                     NRFDAVLIDEAQDFAKTWLQCAKLALKEPDDGDLLIVGDGSQAVFGRRPFRWKDAGIA
                     AVGRTIYKDFDLHVNYRNTREIIEVASIFAGEIKDGDPEQCFSGMKPDASCAIRSGPH
                     PVLLAASSRAAECECAVEQIGAWLASGIEPHEIAILYRALPQDSRSVFTQFVHRLEDL
                     APVYWRKGKMGAPQGAITLCTMHSCKGLQWRALLILWADLLPFSADPEQWKLERGLMY
                     VAMTRAEDELVLTRSGHSPFIDEIEAALSRPEDRDPS"
     gene            complement(4819..5538)
                     /gene="dnaQ"
                     /locus_tag="VNR65_16610"
     CDS             complement(4819..5538)
                     /gene="dnaQ"
                     /locus_tag="VNR65_16610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HWJ20339.1"
                     /translation="MRELVLDTETTGLDPKDNHRVIELACVELINYIPTGVSWHWYFN
                     PERDVPRAATEIHGLTGAFLADKPLFGSVADEILAVLEGARLIIHNAAFDVGFLNHEF
                     GRLGFSPPISFERVTDTLALARRKHPGSPNNLDALCRRYGIDNSTRTKHGALLDSELL
                     AEVYLGLIGAEQAGLNLTSHDAGVKAEGEAAVQVRLKPLPPRLSAEEGAAHVAFVASL
                     GAKAIWLRYLPPATEPAQSAA"
     gene            complement(5557..6183)
                     /gene="coaE"
                     /locus_tag="VNR65_16615"
     CDS             complement(5557..6183)
                     /gene="coaE"
                     /locus_tag="VNR65_16615"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWJ20340.1"
                     /translation="MIVAGLTGSIGMGKSTVAAYLRDHDIPVLDADKIVHDLYAGEAA
                     PLIEAVFPGTTRDGVVDRAALAAKVVASPTELKKLEAIIHPRVRAAEWGFLLDEHDKG
                     SDLAVLEIPLLFETASHTLFDAIIVMSAPADVQRARLLARPGMTADKLDALIARQLPD
                     AEKRAKADFVVDTGVRLEDTYRQIDAVLMEILKRPPLAFQRWADLQED"
     gene            complement(6180..>6457)
                     /locus_tag="VNR65_16620"
     CDS             complement(6180..>6457)
                     /locus_tag="VNR65_16620"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20341.1"
                     /translation="TLGMNGGPPLNVDLSPAARDATVCDIVYSPLETGFLSEARRRGL
                     RAVDGLGMLLHQAVPGFEIWFGVRPEVTPALRETVLAAIEDREKAHA"
BASE COUNT         1467 a         1776 c         1798 g         1416 t
ORIGIN      
        1 ggtcgtcgcg cgcaaagtgc gccgcaaagc gctcaccaca ccggtcgtga ccgatgcctg
       61 aaggccgaac ggattaccca tcgcaacgac aagctggccc acccgcagcg cgtcggaatc
      121 gccgagctga acaggggcga gccgtgcggt atgggcgcga acaagcgcta tgtcggtatc
      181 ggggtcgccg cccaccacat cggcgggcgc ggtgccgccg tcggcgaaca ggacttcaat
      241 cgccctggcg ccttgaatga cgtgattgtt ggtcagaatg tacccgtcgg gcgtgatcac
      301 gatgccggaa cccgcgccgc cctgcatgcg cggattgcct tccggctcgc caggtttcgc
      361 cttgcgctgt atgccgacca tggccgcggc cacgctctcg acgacgccag tgacgatctc
      421 cgagtaggca tccagagctt cctgttcctg gcgcgcatcg cgatcgccag caacggggtt
      481 ggttttcaaa ccgaaactga taggaatgat gttcattgca gccccttggg cagcaaaacg
      541 cgccgcccct tcacttgcaa caaatggtta aggggagccc tttttcaatt tcccgcctgg
      601 cggtcgtgcg cttgtggccg atggtgaatg ttgcacgatg ccgaatagcg gtctgggtcg
      661 aaaccggtaa tgctcacttt gagtaattct agccagctga acctccagaa ccagacctat
      721 catcaatggc acaaatttag ccgagattgg caagcgccgt tcggcgaatt aagagccgga
      781 tcaaatagga tgcgccttca gggcagctct tttatcgata tgcgcgagcg tagagtcggc
      841 aatgcctcgg tacggcctat cgtcttatcg gttgcgagct tcacggcagc gtccgagttc
      901 acggcacaaa ccgaaacgag caccgcccga tctgcaatcg gcgcgttgtc aatggacgcg
      961 tctggaaaga ctgaggctga ggtagcgccg tgcaatatga aaaaattcta tcgggtcaag
     1021 cttaatcgga gatcttttgt acataaattt ggtatagggg cgcggaaagc aagcgtatcc
     1081 tgttgccata taaattcgtg aaggcgtcta ttcctagttt gggacagcca agggggtccg
     1141 ttatcactcc aaacggaata ttgtggcccg cccacagata atcgtcaaaa gcgatcaccc
     1201 caccaattcg tagaaggcga aaactcaata cggcatcgca aatcacatcg gatgcgtaat
     1261 gagagccatc tatgtatata aaatcaaaat agtttctctt tccatcggag aatagtttag
     1321 acaaatgata gtctgaaaat cctttgtgga ttgcaagggc aacgttatga gccgccagtt
     1381 cagatgataa tttggtgttg tgcagaaacc tggcttcaac ctctgacatg tttgtgccgt
     1441 cggatttgtg ctcggcgctg ccttcccaac tatcgataca gtggacctca atctctttct
     1501 tttgggctaa gagatcaatg agataacaag tgcttgcccc ctcatatgac cctatctcga
     1561 ggatcgttgt tggattgaga ttcgttatca gttgatccca cacacccttt gccagattat
     1621 tgaaccagct gttcgtgaac tgatgagcgg ggtgttcttg ttggttcatg gctttgcgtc
     1681 tctggcgttt gggtctgggt tcgcatgctc ggcagctgaa gccttcacga aagcctattt
     1741 aaaagagatc cacctgggtt gcccacaatg cagaacgact gcttattcat ttgtgcagat
     1801 cgtaacgctg atcatggccg cgtcgctgat cttgaccatg ctccccaacg aaattgcggg
     1861 atacctggag acatcatcat gcgtccagag ctgcggacgg ttctaacagg ttgtaaaaaa
     1921 cgatacggct cgtagtgcga caagctcaca atgggggtcc aaacctttga aatagcttgg
     1981 cctcatcctt gtcgagggat gtagcctgag ttctcacctt cttcagcatc cagcaaacta
     2041 acttcggctt caatttatca tttagggcat gtatgaaact ggtagctaag gcgggcgtgg
     2101 gattttctgc ctgttggaat gggagacatg cgagtgcgtt agtaaaaatg acagcagtgg
     2161 gccgagtgcc tcccaagaca aaattacgaa agagcaaaaa gctgcgtttc gaatgatggg
     2221 cgagtgtcct ggcggtaacg gcggctcttc caccaaacag gaggcgtact acggaataag
     2281 ccggatgatc ggaatcggcc tcttgcacgc gattcagcga acggcgcaaa acccggcccc
     2341 aacagctgac ggatcgttgc cgttctccgc gccggccgag cgtttaacgc aatagggtta
     2401 acaggagcgc gccggatttc cttccggtca ccatcatgct taaagatccg ttcattctgg
     2461 cgcaaaagcc aagcgccgct gcctcaatgt ggcaggaaat aatcgggcta ggctattgaa
     2521 tataaataat ttttctccgt ataagacctt ccattaaata gaatttaatg ccagaatttt
     2581 ctcatttact gggacacgca aaagtccgtt aaatatgttt cggcttggcg aattaacgct
     2641 tgcacgcagc cggaacagct gcggcgcggg cggcggcgat ggtgtccggt tcggtatttt
     2701 tcccacgatt atcaagggtt ggagcgggct ggagctttcg ttaacctgcc ggcaaggcga
     2761 attcgctgag gatctagaag aagaaggaag gcggacgaaa gcgtaaggga caagttgaca
     2821 acagatgccg ccattcacag aatgcataca aaacgcgagc acctcttatg aggggtctct
     2881 atcctccggc cggctgagcg ctgcctcgat ctcatcgatg aacggcgaat ggcctgacct
     2941 tgtgagaacg agttcgtctt ccgcgcgtgt catggcgaca tacataaggc cgcgttcgag
     3001 cttccactgt tccggatcgg cggagaacgg gagcaagtct gcccaaagga tcagcagggc
     3061 gcgccattgc agccccttgc aggaatgcat ggtgcagagc gttatggctc cctgcggcgc
     3121 gcccatcttt ccttttcgcc aatagacagg agcaaggtct tccagcctgt gcacgaactg
     3181 agtgaagacg gatcggctgt cttgcggcaa ggcgcggtaa agaatggcga tctcatgggg
     3241 ctcaatgccg gaggccagcc aagccccgat ctgttcaacg gcgcactcgc attcagcagc
     3301 ccggcttgac gccgccaata gcaccggatg tggaccggac cggattgcac aacttgcgtc
     3361 aggcttcata cccgaaaagc attgctccgg gtcaccatcc ttgatttcac ctgcaaaaat
     3421 ggaagcaacc tcgataatct ctcgcgtatt gcggtaattc acatgcagat caaagtcttt
     3481 gtaaattgtg cggcctaccg cggcgatccc ggcatctttc catcggaatg gtctcctgcc
     3541 gaacacggcc tgacttccat caccaacgat gagcagatcg ccgtcatcag gctctttcaa
     3601 cgccagcttc gcgcattgga gccaagtctt ggcgaagtct tgagcttcgt cgatcaggac
     3661 agcgtcgaag cggttcgcat ctccctcccc acgttccagt cgctcaagaa ggcgttgacc
     3721 atagtagtcc acatcctcct ttttatcgaa gcagaccccg tgtcgtgatc cccaaagatg
     3781 gaagttgtgg cagattatat ttttctggct atcgaagata ctctggatat gttcagcgag
     3841 tggcctgttg tagcaaagaa cgagtacctc tttgtttgct tcggcagcca gcaggcgagc
     3901 tctcgctatc agaattaccg ttttaccgga gcccgcaaca ccatataaaa tgcggtggcc
     3961 ttcaccgata ttccgttcga aacgctcctg gcgaaggtcc aaggtcttca acgtttcgcg
     4021 gcttccatcg cgtccaatcg gcgctttcga gatgacgatc tctggatgca tgatggcccg
     4081 taacgcagaa atttgcgtat cattcaaggc tgcaaaatcc caccagggat cgaagaagtt
     4141 tttgagggca ggaaccagac cttccgtttc aatattggca aggtgttcga actcatccct
     4201 cgtcatgact ttggaaggcg gaaagagcgg cgttaagcca agactatcca actgatcccg
     4261 tttgatgttg ttgagaacca caagattgcc gagaggaaag atcaactgcc ctgcatgggc
     4321 gccgctgctc cgcaacagag gagtggagtt gttgaaggct ttagccgcgt ccatcaaccc
     4381 aaacatgtaa tcccgagcct gccggatcgg gtgtctctgg gtctcagttc ttcctctgct
     4441 ggtgatcctt acctgattga gatccccact ttcgatatga ccgggatacc atcccttaac
     4501 ttcgatgatg agcagcccca cctcagggat aatcacaatg aaatctgggt acttgttgct
     4561 tacaactggc tcgtaataga caatgcagtt gtcgggcagc cgctggagca cctcgaaaac
     4621 gcgcttctcg cccgcgctcg ccccgctcgg caagcgatcc ggtatcatct cagccattgg
     4681 ctttctagcc cctgctcaac tggcaaaaca ataaacatac acagagttgc ctttacagca
     4741 attctgcttt tctattccag aaagcttaga atttgaagaa atagcgatcg tgaaggctaa
     4801 cgccaatcca agtgccgttc aagccgccga ttgggctggc tctgtcgcgg gcggcaagta
     4861 acggagccaa attgccttcg cgccaagcga ggcgacaaag gctacgtggg cggcgccttc
     4921 ctcagccgaa agacgcggcg gcagcggctt caaccggacc tgcaccgcag cctcgccttc
     4981 cgccttaaca ccggcatcgt gagaggtgag gttcagcccg gcctgctcag cgccgatcaa
     5041 cccaaggtag acctcggcca gaagctcgct atcgagcaac gcgccgtgct tcgtgcgcgt
     5101 ggagttgtcg atcccgtagc gccggcagag agcgtcaaga ttgttcgggc taccgggatg
     5161 cttccgccgg gctagcgcca gcgtatcggt gacacgttcg aagctgatgg gcggagaaaa
     5221 gccaagccgg ccgaattcgt gattgagaaa gccgacgtcg aaagccgcgt tgtggatgat
     5281 gaggcgcgcg ccttccagca cggcgaggat ttcatccgct acggagccga agagaggctt
     5341 atccgcgagg aaggccccgg tgaggccgtg aatttccgtc gccgcccttg ggacatcccg
     5401 ctccgggttg aaataccagt gccaggaaac gccggtggga atgtaattaa tcagctcgac
     5461 gcaggccaac tcgatgacgc ggtgattgtc tttggggtca agcccggtcg tctcggtgtc
     5521 gagcacaagt tcgcgcattg agccccatcg atgcggtcag tcttcttgga ggtcggccca
     5581 gcgctggaat gccagcgggg gccgtttcag gatctccatc aggactgcat cgatttggcg
     5641 gtacgtgtct tccagtcgaa cgccggtatc cacaacgaag tcggctttgg cgcgcttttc
     5701 ggcatcaggc agttgccgcg cgatcagggc atcgagtttg tctgcggtca tgccgggccg
     5761 cgccaaaagg cgggcgcgtt ggacgtcggc cggggcgctc ataacaataa tggcgtcgaa
     5821 aagagtatgc gacgctgtct cgaagagaag cggtatttcg aggacggcaa ggtcggagcc
     5881 tttgtcgtgt tcgtccagca gaaagcccca ttcggcagcg cgaacgcgag gatggatgat
     5941 ggcttcgagc ttcttcagtt cggtcggcga cgcaacgacc ttcgcggcta aagccgcccg
     6001 atccacgacg ccatcccggg ttgtgccggg gaacaccgcc tcgatcagcg gcgcggcttc
     6061 acccgcataa aggtcgtgaa cgattttgtc agcgtccaaa acgggaatgt catgatcgcg
     6121 aaggtaggcc gcgaccgtgc tcttccccat tccgatcgag cctgtgaggc ctgcgacaat
     6181 catgcgtgcg ctttctcacg atcttcgatg gcggcaagca cagtttcacg caaagccggg
     6241 gtcacttcgg ggcgcacgcc aaaccagatt tcaaagccgg ggaccgcttg atgaagaagc
     6301 atgcccagcc catcgacggc cctcaaccca cgacgccgag cctcgcttaa gaagccggtt
     6361 tcaagcggtg aataaacgat gtcgcaaacc gtagcatccc gcgcggctgg ggaaagatcg
     6421 acattgagcg gcggaccgcc gttcatgccc agcgtcg
//
LOCUS       DATMGX010001014         2124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_112111, whole genome shotgun sequence.
ACCESSION   DATMGX010001014 DATMGX010000000
VERSION     DATMGX010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112111"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VNR65_16625"
     CDS             <1..260
                     /locus_tag="VNR65_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20342.1"
                     /translation="KVGTDEYCVTQKGKGKRTAVMVGGGAGGGALIGGIAGGGKGALI
                     GGLVGAGAGTAGAAFTGNKELSIPAESAVSFRTTTAITIQP"
     gene            379..1707
                     /locus_tag="VNR65_16630"
     CDS             379..1707
                     /locus_tag="VNR65_16630"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HWJ20343.1"
                     /translation="MTKSLPLTIRHAMYNLRGGFLIRPLTIALSLGCAGAFLSWLEEQ
                     FPVTSAWVPTVLFPSHSDPQVAQVILAGIAGSIMTVVSIVFAILLMTLTLASMQFSPR
                     IIVSFSRDRVTQWTLGIFLGTFSYCMAALPAAHSLPHPFAPVATVLGAMVLALACVGL
                     LLFFIHHISEAISVNHIVDRIAEETEAMIDEIMPGPHRPNHLKDAAPLRPNPSEVAVL
                     SNVSGYIRFVDKRRVVAVAKHYHVSIRVLRRVGHFVPAGIPLMMVSKGDRLPPEGTSE
                     LLSAFDCGPTRTLQQDVEFGVLQIVDVALKAISPAVNDPTTAICCVDQLSRILIRFAS
                     REPPEELLYDPPGIVRASIGWIHFERLLEAAFEQIRMYAKTDVAVSLRLLRALDDIAA
                     STPDPEFRRILVEQGTRTVAGCAEKLGEEELRGLRARQAALESFTAISQP"
     gene            complement(1776..>2124)
                     /locus_tag="VNR65_16635"
     CDS             complement(1776..>2124)
                     /locus_tag="VNR65_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ20344.1"
                     /translation="RANLRRARAEASELEPIEAGDFKIDRGARTVFVKDRAVHLTPKE
                     FDLLAYLARHAGKVVTHRALLGAVWGGESTEQVEYLRVFVAQLRKKLEPSPNSPRYIV
                     TDPWVGYRFEPGD"
BASE COUNT          383 a          675 c          645 g          421 t
ORIGIN      
        1 acaaagtggg caccgatgaa tattgcgtta cccagaaggg caagggcaag cgcacggccg
       61 tcatggttgg cggcggtgcc ggcggtggcg cgcttatcgg cggaatcgct ggaggtggca
      121 agggcgctct cattggcgga ctggtcggcg caggcgccgg caccgcgggc gcggccttca
      181 ctggcaacaa ggaactcagc attccggcag agtccgcggt cagcttcagg accaccacgg
      241 cgatcaccat tcagccgtag tcgtaatttg tttgagcccg actaacggct cgactcctaa
      301 cagctaaggg aggctttcgg gcctcccttt cttctttgta cgcgcaggtg tgggccttac
      361 actgaaacgg agagggccgt gaccaagagt ctgccactga ccattcgcca cgccatgtac
      421 aacctgcgtg gcggcttttt gattcgcccg cttacaatcg cgctatcgct gggttgcgcg
      481 ggcgcgttcc tgtcgtggtt ggaagagcag ttcccggtca ctagcgcctg ggtgcccact
      541 gtcctattcc cctcgcactc cgacccacaa gtcgcccagg tcattctggc gggcatcgcc
      601 ggctccatca tgacggtggt gtccatcgtg ttcgccatcc tgctgatgac gctcaccctg
      661 gcctcgatgc agttctcccc gcgcatcatc gtcagctttt ccagggaccg cgtgacgcaa
      721 tggacgctcg gtatcttcct cggcactttc tcgtactgca tggcggcgct tccggcggcg
      781 cactcgctcc ctcacccctt cgcgccagta gccaccgtcc tgggcgcgat ggtgctggcg
      841 ctggcctgcg tcggtttgct gctctttttc attcaccaca tctcggaggc catcagtgtc
      901 aatcacatcg tcgacagaat tgcggaagaa accgaagcca tgatcgatga gatcatgccc
      961 gggccgcacc ggccgaacca cctgaaggat gctgcaccgc tcagacccaa cccgagcgag
     1021 gtggccgtct tgagcaatgt ttccggctac atccgcttcg tcgataagcg acgcgtggtt
     1081 gccgttgcga agcactatca cgtcagtatc cgcgttctgc gtcgggtcgg ccacttcgtt
     1141 ccggcgggca tcccgctgat gatggtctca aagggggacc gtctgccccc cgaaggaacc
     1201 tccgaactgc tttccgcgtt cgattgcggc ccgactagaa cgttgcaaca ggacgtggag
     1261 tttggcgtcc tgcagattgt cgatgtggcg ttgaaggcga tttcgcccgc ggtcaacgac
     1321 cccaccaccg ccatctgctg cgtcgatcag ttgagccgca ttctgatccg cttcgcgtcc
     1381 cgagagccgc cggaggaact gctctacgat ccgccgggca tcgttcgcgc cagcattgga
     1441 tggatccact tcgagcgcct gctggaggca gcctttgagc agatccgcat gtatgcgaag
     1501 acggacgtgg ccgtcagctt gcggttgctg cgagccctcg atgacatcgc cgcctcgacg
     1561 cccgatccgg agttccgccg gatactggtc gagcaaggaa cgcggaccgt ggcaggctgc
     1621 gccgaaaagc tcggcgaaga ggaactgaga gggttgcgcg ctcgccaggc cgcgctggag
     1681 agcttcaccg cgatctcgca gccgtagatt agaacagagg ggcacaccgt atccgaggcc
     1741 aggcagaacc agcagccggc ttcaggctca aggactcaat ctccgggctc gaagcgatac
     1801 cccacccagg gatcggtcac aatgtagcga ggggaatttg gcgagggctc aagcttcttg
     1861 cggagttgcg caacgaacac gcgcaggtat tcgacctgtt ctgtgctctc accaccccag
     1921 accgcgccca gcaaggcacg atgtgttacc actttgccag cgtggcgggc cagatacgcg
     1981 agcaagtcaa attccttcgg agtgaggtgg accgcgcggt cctttacaaa aacggtgcgg
     2041 gctccgcgat caatcttgaa gtcgcccgct tctatcggct ccagttccga cgcttccgcc
     2101 cgagcgcggc gaagattggc gcgg
//
LOCUS       DATMGX010001015        12105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_343473, whole genome shotgun sequence.
ACCESSION   DATMGX010001015 DATMGX010000000
VERSION     DATMGX010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343473"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..302)
                     /locus_tag="VNR65_16640"
     CDS             complement(111..302)
                     /locus_tag="VNR65_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20345.1"
                     /translation="MARTTILDGIAAEQVRNVTYLHEEVTAEIHAPTARHNFACILSA
                     TIDSVPKCMVAPARPSSGK"
     gene            426..656
                     /locus_tag="VNR65_16645"
     CDS             426..656
                     /locus_tag="VNR65_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20346.1"
                     /translation="MKLISELKLKLFADEMRRQARRIQTLDGQVNVKIPVAALGGSIL
                     SGPQLVGKNLAEYWLEAREMFRDDGAKVEYSL"
     gene            656..1333
                     /locus_tag="VNR65_16650"
     CDS             656..1333
                     /locus_tag="VNR65_16650"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWJ20347.1"
                     /translation="MADSSDLSAYYQHATSFASQISWYMRRRMFATFMEYFKPGPETL
                     ILDVGVTSDDSFQESNYFEQLYPYPHRITCVGTEDGSHLEARYPGLTYRRVQPGQPLP
                     FARSAFDIVFSNAVIEHVGSRAAQSGFVRELGRVGKAFCITTPNRWFPVEHHSGVPLL
                     HFLPVNVYRGLLRRTRYRYWAQEENLNMLTEAGLAGLFPPDWHTAIKRIRVAGVCSNL
                     VALGKSA"
     gene            1694..2509
                     /locus_tag="VNR65_16655"
     CDS             1694..2509
                     /locus_tag="VNR65_16655"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ20348.1"
                     /translation="MSDKVLITGGSSGIGKQLAADYLRRGAHVTIVSNNTAKLQSACS
                     ELAAISPSICALACDVTELGEVRQLAEEYLRQFGPPAVLVSNAGFAVYRLFVEMPAEE
                     IQRLLTVNFVGGCLITREFLPAMVAAGHGDVVVMASIAGRLVMTPCGVYSAAKHGLVA
                     WAHTLRLELKQTGVRVHVICPGRVETDFFAHETFVQRDRRAETRFTIPVEIVSRATIA
                     AIQKNRFMTYVPSSYGLLVWLANSFPFPVRMLLDRLMEERVRALFPARRNGAQ"
     gene            2506..3372
                     /locus_tag="VNR65_16660"
     CDS             2506..3372
                     /locus_tag="VNR65_16660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ20349.1"
                     /translation="MTQCTICGSARPRLLCRKGGAVYHFCPECRVIFQYPPPPPDAML
                     SYADTEYEDGLYRDYVEAREMKLVHFRYRLERLLPLVRCGRLLDIGCSCGYFMEAAAE
                     RGFEVEGLEFSGAAIAAADASVRPRISCSSVDAFDSSRSYDLITAFDLIEHVPQPQDF
                     LRKVRRLLAPGGWLVMTTPDAGHFLRHVMASNWPMLQPMQHLTIFSRQAMRLALEEAG
                     FDDIRFERATKTISYDYLTDQLRTLTPALHKGLRAVGRCLPARTRRKYRHVSIGEFMV
                     MARPADARETNQ"
     gene            3369..4982
                     /locus_tag="VNR65_16665"
     CDS             3369..4982
                     /locus_tag="VNR65_16665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ20350.1"
                     /translation="MIKTISRSSRVKGATGRMQRECPVCGCAELDYEFIVENCPLCCC
                     RDCSLTFLNPQPEAPEPSGAHPLEADEGIYEIHAANAAKRLDQFISYAGTSSSRLLLV
                     GAGKSLRDEADRRGFVVESLSPAELEDRGQKDGAGSFAGCILYCALEQTSDPLACLAV
                     VRSLLSPDGCLMVIAPTLDSRTARMFRSTWWEFKRHNRYYFTSDTMQSLLIKGGYGDP
                     IILPDESVVSLQYMRRKLATLPAAGRYRLLRLLTAASPGFVRHKAFRFLHSRLIFMAR
                     LKQVAPAPRLSVIVPLYNESSTFPKLMEMLVEKQIPNMEVEIILVESNSSDGSRELAL
                     AYRDHPRVKLILEDRPSGKGHAVRNGLQVATGDIVLFQDADLEYDLDDYEGLIEPILG
                     YRQNFVIGSRHTMSTRLWKIRSFDDAGGLAAVFNLGHLIFLMLFNVIYRQRLKDPFSM
                     FKVFRRDCLYGLQFECNRFDFDFEIVIKLLRKGYRPMELPVNYRARSFSEGKKVTIFR
                     DPLTWLRALVKYRNSPLYDLSVPEDTARS"
     gene            complement(4987..5706)
                     /locus_tag="VNR65_16670"
     CDS             complement(4987..5706)
                     /locus_tag="VNR65_16670"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWJ20351.1"
                     /translation="MKEPVLPVAVLAGGLATRLRPLTETIPKALIELNSEPFVAHQLR
                     LLSSRGIDRVVLCVGYRGEMIRDFVGDGSRFGLRAEVLFDGPTLLGTAGAIRHALPLL
                     GANFFVLYGDSYLPCDYSAVARAFLSSGKRGLMTVFRNDGNFDASNVEYCDGQIVRYN
                     KRSTTPAMRHIDYGLGAFRADVFAELRPGVHADLAAVYQRLLSAGDLAAFDVPERFYE
                     IGSVRGLEETSEYLRRVSGKL"
     gene            complement(5703..6632)
                     /locus_tag="VNR65_16675"
     CDS             complement(5703..6632)
                     /locus_tag="VNR65_16675"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20352.1"
                     /translation="MPSYYRQHGGFVIAAAIDKYVYITLHQTFVEEAIIKYSQTERVK
                     RIEDIHHPLVREALLLTGVPPEGLEIASMSDIPAGTGLGSSGSFTTALLQALHTHKKT
                     IVPKQELAEQACHVEIDLAQEPVGKQDQYIAAFGGITCFEFLPDDRVQVDPVRLNSET
                     LSNLEDNLLLFFTGYTRSASQILADQDRRTRSTDQSMIDNLHFVKELGYQSRCALESG
                     NLRQFADIMHVHWEHKKKRSGAMSNRSIDEYYELARANGSLGGKLIGAGGGGFLMFYT
                     EDKTRLRRAMLAAGLREVRLRFDFQGTTVVTQS"
     gene            complement(6683..7663)
                     /locus_tag="VNR65_16680"
     CDS             complement(6683..7663)
                     /locus_tag="VNR65_16680"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWJ20353.1"
                     /translation="MRVAIIGCGLIGQKRALALPQGWSLALCCDQVRERAENLARFYP
                     GAQITIEAESAAANPGVDVVIVATTHDVLAPLAARAAGAGKHVLLEKPGARCSAELDP
                     VADAARSTGALVRVGFNHRYHRAFRKARAILDTGVLGEPMFVRARYGHGGRPGYEKEW
                     RAVPEISGGGEAVDQGVHLIDLARWFLGEFVVVQGAAPTFYWNMPVEDNAFFLLQTES
                     GRVAFLHASWTEWKNLFSFEYSGRCGKLEIAGLGGSYGTERLAHYQMLPGMGPPETTI
                     YEYPAADDSWETEMADFSEDIRLQRTPLPGIVDAQAALRIAEQIRMGRQP"
     gene            complement(7666..8529)
                     /locus_tag="VNR65_16685"
     CDS             complement(7666..8529)
                     /locus_tag="VNR65_16685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ20354.1"
                     /translation="MKLANKVTLVTGASQGFGYVIARRFVQEGASVLLCARTADDLEG
                     AAEGVRAAAAPGAQVFAERADVSSEADVGRLAEVIKKQFGKLDILVSNAGVYGPKGAV
                     DEVSWSDWSQAISINLLGTVLCCRAFLPLLRQATRGKILLVSGGGATKPLPFLSAYAA
                     SKAAVVRFGETLAEELRGAGVDVNSIAPGALNTRLLAEVLSAGPEKVGQTFYEASLRQ
                     KESGGTPLERGAELCVYLASSESDGITGKLISAVWDPWADFAEHRDDLQKTDVYTLRR
                     IVPGERGLSWG"
     gene            complement(8526..9848)
                     /locus_tag="VNR65_16690"
     CDS             complement(8526..9848)
                     /locus_tag="VNR65_16690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWJ20355.1"
                     /translation="MKVAVAGLWHLGMVTAACLASRGHNVLGFDEAPIIERLQQNIMP
                     VAEPGLRELAAKALSDGRLAYTANASGLAAAQVLWVCYDTPVDKNDVADSEYVVNQTL
                     SLLPYLATGSLVLISSQLPEGTTARLESCWHKNGGSELRFAYSPENLRLGKAIEVFTN
                     PDRVVAGVRTHEDRETIAALLSPFTDRIEWMSVESAEMTKHALNAFLATSVAFINEIA
                     ALCEKVGADAEEVERGLKTDVRIGSRAYLHPGSAFAGGTLARDIAFLLEIAERQKVNG
                     YLLAGVWQSNGFHKGWLCRRMPEVLGELAGRRIAILGLAYKPGTDTLRRSGAVEAATW
                     LTQQGATVSAFDPAVRALPDELAATMSLRSNAPDALSGADAVLIATPWPEFRDISVDL
                     LLTRMREPVVFDPGRHLQQALGADSRVRYFAVGRGDPLMRQGDIGQTA"
     gene            complement(9845..10801)
                     /locus_tag="VNR65_16695"
     CDS             complement(9845..10801)
                     /locus_tag="VNR65_16695"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWJ20356.1"
                     /translation="MRFFVSGCAGFIGSSLTDRLLAANHQVVGYDNFSTGRAGFLESA
                     SRSERFTLITGDLLDTEKVTRAMAGCEFIFHLAANADVRFGTNHPRRDLEQNTIATVN
                     VLEAMRVNGVRDIAFSSTGSVYGEASCFPTPENASMPVQTSLYGASKLAAEGFIEAFC
                     EAFGMRAFILRFVSILGERYTHGHVLDFYKQLRAHPEYLEVLGNGSQRKSYLYVQDCL
                     DAMLLAIESAPGKVNIFNLGLDEYCEVNDSIRWICGRLGLAPELRYSGGDRGWAGDNP
                     FIFLDCTAIGSLGWRPKLNIQQAVIRTVEFLEQNPAITEVVA"
     gene            complement(10828..11127)
                     /locus_tag="VNR65_16700"
     CDS             complement(10828..11127)
                     /locus_tag="VNR65_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20357.1"
                     /translation="MFGTAGVASGNATQDDQVGIDRLIEMIASCFSRRDGVVNEAPLR
                     DGNLYPPKDAASLRIPAGTAEALGRLKRRGYQQLFSTDFDAIGYARVKVSLLVHI"
     gene            complement(11364..11657)
                     /locus_tag="VNR65_16705"
     CDS             complement(11364..11657)
                     /locus_tag="VNR65_16705"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWJ20358.1"
                     /translation="MVSHSDSRTSFSAQFLTEVQEVARKLDAVAIEKMAALLADLRER
                     GGRLFILGVGGSAGNASHAFNDFRKLAGIESYAPTDKVSELKVGRPCSKRGCE"
     gene            complement(11651..11893)
                     /locus_tag="VNR65_16710"
     CDS             complement(11651..11893)
                     /locus_tag="VNR65_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20359.1"
                     /translation="MRLRASSHLLKDGKLAAFEVQERLYEIGSPDGLRDTAEFLAAGD
                     QHHTIRLHSQRRPRAWRLQDHPILQFRPNERLTDSW"
BASE COUNT         2470 a         3671 c         3530 g         2434 t
ORIGIN      
        1 gccgcaagga actcgaaatc ttcacacaag ccctccgaag aaccgatttc atagaagcgt
       61 tcctgaatct ggaaggcggc aagctcacgc taactgacgt caatcgttga tcattttcca
      121 gaagaaggac gcgcaggtgc gaccatacat tttggtacac tatcgatggt cgcggaaagt
      181 atacaggcga aattatggcg cgcagttgga gcatgaattt cggcggtgac ttcctcgtgg
      241 aggtacgtga cattgcggac ctgctcggcc gctatgccat cgaggatggt ggtacgcgcc
      301 atgaccggca tccgcgaccg tctgggccgc ttgtttatcc tcaaagtcgg gggggggcag
      361 cgctgccaac gcgttgcacg cggtaaacga tttccgcaaa tttgcgaggc tgcacaactt
      421 ttcctatgaa gctgatctcc gaactgaaac tcaaactgtt cgctgatgaa atgcgccgtc
      481 aggcgcgccg gattcagacc ttagacggac aggtcaatgt gaagatcccg gttgccgcgc
      541 tcggaggatc catcctgagt gggccccaac ttgtcgggaa gaacctggct gagtactggc
      601 tggaagcgcg ggaaatgttc cgggacgatg gtgccaaggt ggagtattcg ctgtgatggc
      661 agacagttcc gacctttccg cttattacca gcacgcgacc agtttcgcgt cacagatttc
      721 ctggtacatg cggcggagga tgttcgccac attcatggag tactttaagc ctgggccgga
      781 gacgctgatt ctggacgtcg gcgtcaccag cgacgattcg tttcaggagt cgaattattt
      841 cgaacagcta tatccgtatc cgcaccggat cacctgcgtc ggaacggagg atggctccca
      901 cctggaggcg cgctaccctg gactgaccta tcgccgcgtg cagcccggcc agccactgcc
      961 gttcgctcgc tcggccttcg acatcgtatt cagcaacgcg gtaatcgaac acgtgggtag
     1021 ccgcgcagct caatcgggct ttgttcgcga actgggtcgt gtggggaagg ctttctgcat
     1081 caccaccccc aaccgttggt ttccggtcga acatcactcc ggcgtcccat tactgcactt
     1141 cctgcctgta aacgtttatc gtggcctgct gcgccgaaca cggtaccgat attgggcgca
     1201 agaagaaaat ttgaacatgc tcaccgaagc gggtctggcc gggctatttc cacccgactg
     1261 gcatacggcc atcaagagga ttcgcgtggc gggtgtgtgt tccaacctgg ttgccctggg
     1321 aaaaagcgcg tgaaggctcc ggcgagttca cgggggaagt ctacgacatc agaatctgtg
     1381 gccgggaagg cggaaccgaa accgcgcccc ttaagtccat tagggggggt gctgagcagc
     1441 gagaccgctc gagctccgct gattctgttc gtcattttga aaccggagtt ttactcgtcc
     1501 ccgcggggga gccgaaccat tacgatgagc gcgagcacgc cgccgcgaag agtgtacgac
     1561 gacgcgcgtc cgttgtcccg cctcggatgc ccgtacccgg acctacccga gtcgcaattc
     1621 gagccggcga tggcccttag ccatcaacag ttttggcggc gtgcacgggt tgccatcacc
     1681 gatggaggcg gggatgtctg acaaggtgct aatcaccgga ggctcgagcg gcatcggcaa
     1741 gcagctcgcc gcggattatc tccggcgcgg cgcgcacgtg accattgttt ccaacaacac
     1801 agctaagctc cagagcgctt gctccgaact cgcggcaata tcgccttcga tctgcgcgct
     1861 ggcatgcgat gtcacggaac tgggtgaagt gcgccagctg gcggaagagt accttcggca
     1921 gtttggtccg cccgctgtgc tcgtgagcaa tgccggtttt gctgtgtacc ggttgttcgt
     1981 ggaaatgccg gcggaggaga tccagcgcct cttgaccgta aacttcgtcg gtggctgcct
     2041 gatcacgcgg gagttcctgc cagcgatggt ggcggccggc cacggcgacg tcgtcgtgat
     2101 ggcctcaatt gcgggccgcc tggtgatgac accgtgcggc gtatacagcg ccgcgaagca
     2161 cggcttggtt gcttgggcgc acaccttgag actggagctc aaacaaaccg gcgtgcgcgt
     2221 gcatgtgatt tgcccaggcc gtgtggagac cgactttttc gcgcacgaaa ccttcgttca
     2281 gcgtgaccgc cgggccgaga cccgtttcac catcccggtt gaaatcgtct cacgagcaac
     2341 aatcgcagcc attcaaaaga accgctttat gacctacgtg ccaagttcct acggtctgct
     2401 ggtttggctg gcgaattcgt tcccgttccc tgtacgcatg ctgctcgatc gcctgatgga
     2461 ggagcgcgtg cgtgcgctgt tcccggctcg gaggaatgga gcacaatgac acaatgtacg
     2521 atctgcggca gcgcgcggcc gcgtcttctg tgccgcaagg gtggcgcggt gtaccacttc
     2581 tgcccggaat gccgcgtcat cttccaatat ccaccgccgc cgcccgatgc gatgctctcc
     2641 tacgccgaca ccgagtacga ggacggctta tatcgcgact acgtcgaagc ccgcgagatg
     2701 aagctcgtgc acttccggta ccgtctggaa cggcttcttc cgctcgtgcg gtgcggccga
     2761 ctgctcgata tcggctgctc atgcggctac tttatggaag ctgcggccga gaggggcttc
     2821 gaagtcgaag gcctggagtt ctcgggtgct gcaatcgccg ccgctgacgc ctcggtgcgg
     2881 ccgcgcattt cgtgctccag cgtggacgcg ttcgacagca gccgctccta cgatctgatc
     2941 accgccttcg atctgattga acacgtgcct cagccccagg acttcttgag gaaggtccgc
     3001 cggctgctgg cgcccggggg ctggttggta atgacaactc ccgatgccgg acacttcctc
     3061 cgtcatgtga tggcgtcgaa ttggccgatg ctccagccga tgcaacacct gacgattttc
     3121 tcgcgccagg cgatgcgcct ggcgctcgaa gaagccggat tcgacgacat ccgttttgaa
     3181 cgggctacaa aaacaatcag ttacgactac ctgaccgatc agctccggac attaactcct
     3241 gcgttgcaca aggggctgcg cgctgtgggc cgctgcctgc cggcacgcac aaggcggaaa
     3301 taccgacacg tcagcattgg ggaattcatg gttatggcca ggccggccga cgcacgggaa
     3361 acgaaccaat gatcaagacg attagcaggt cttcaagagt gaaaggtgca accggccgca
     3421 tgcagcgtga atgcccggtt tgcgggtgtg ccgaactgga ctacgagttc attgtcgaga
     3481 actgccctct ctgttgctgc cgcgactgtt cgttgacatt cctgaatccg cagccggagg
     3541 ccccggagcc gtccggcgcg cacccgctcg aggccgacga aggtatttac gagatccacg
     3601 cggcaaatgc cgcaaagcgg ctggatcagt tcatttccta tgcagggacc tcatccagcc
     3661 ggttgcttct cgtaggagct gggaagtcgc ttcgggacga ggcggatcgg cgcggcttcg
     3721 tggtggagtc cctctcgccc gcggaactcg aggaccgtgg gcagaaggat ggcgctggct
     3781 cgttcgccgg ctgcatcctg tactgtgctc tggaacagac cagcgatccc ctggcctgcc
     3841 ttgccgttgt ccgctcgcta ctgtctcctg atggatgcct catggttata gcccccacgc
     3901 tggacagccg cactgcgcgc atgttccgct ccacgtggtg ggagttcaag cggcacaacc
     3961 ggtactattt cacttcggat acgatgcaga gcctgcttat caaagggggc tatggcgatc
     4021 cgatcatctt gcccgacgaa tccgtcgtgt cgctgcaata catgcggcgg aagctggcga
     4081 ctctgccggc tgcgggccga taccgactgc tgcggctact gaccgcggcc agtccgggtt
     4141 tcgttcggca caaggcgttt cggttcctgc acagccgatt gatttttatg gcccgtctga
     4201 agcaagtcgc gcccgctccg aggctctccg tcattgttcc gctgtacaac gagagttcga
     4261 catttcccaa actgatggaa atgttggtcg agaagcagat tcccaacatg gaggttgaga
     4321 tcattctcgt ggaaagcaat tccagcgacg ggagccgcga actagcgctc gcctaccgcg
     4381 accatccgcg cgtaaagctg atcctggaag accggcctag cggcaaggga cacgcggtga
     4441 gaaacgggct tcaggttgca accggagaca ttgtcctgtt ccaggacgcc gatctggaat
     4501 acgatcttga cgattatgag ggcttgattg agccgatcct gggctatcgt caaaatttcg
     4561 tgatcggctc gcggcacacg atgagcacgc ggctatggaa gattcgcagc ttcgatgacg
     4621 ccgggggcct ggctgcggtg ttcaatcttg ggcacctgat atttctgatg ctgttcaacg
     4681 ttatctaccg gcagcggtta aaagatccct tcagcatgtt caaagtgttt cggcgcgact
     4741 gtctatacgg cctccagttc gaatgcaacc ggttcgactt cgattttgag attgtgatca
     4801 agctgctccg caagggttac cgcccgatgg agctgcccgt gaactaccgc gcacgttctt
     4861 tctcggaagg caagaaagtg accatatttc gcgatccgtt gacctggttg cgggcgctcg
     4921 taaagtatcg taactcgccc ctttacgatc tcagcgtgcc tgaggacaca gcgcgatcat
     4981 aaccgatcac aactttccgg agaccctccg caaatactcg gacgtttcct ccagacctcg
     5041 aaccgacccg atctcgtaga accgttccgg gacatcaaaa gcagccaggt ccccggcgct
     5101 taggaggcgc tgatatacgg ctgcgaggtc ggcgtgaacg ccggggcgca actcggcgaa
     5161 aacgtctgca cgaaacgcac ccaagccgta atcgatgtgc cgcatcgccg gcgttgtgct
     5221 cctcttgtta tagcgaacga tttgtccgtc gcagtactcg acattgctgg cgtcgaagtt
     5281 gccatcattg cgaaaaaccg tcataaggcc acgcttgcca ctgctgagga acgctctggc
     5341 tacggcagaa taatcgcaag gtagatagga gtcgccgtac aggacaaaga aattcgcgcc
     5401 tagcaacgga agagcgtggc gaatggcgcc agcagtgccg agcaacgtcg gtccgtcgaa
     5461 cagcacctcg gcgcgcagcc caaagcgcga accgtcgccg acaaagtccc ggatcatctc
     5521 gccgcgataa cctacgcaca gcacgacccg gtcgatgcct cgggaggaca atagccgcaa
     5581 ctggtgcgcg acaaacggct cgctgttcag ctcgattaag gccttgggga tagtctcggt
     5641 caggggtcgc agacgcgttg ccaggccccc ggcaaggacg gctaccggaa gtacgggttc
     5701 cttcacgact gggtcactac agtggtgcct tggaagtcaa accgaagccg aacctcgcgc
     5761 aggcctgcgg ctagcatggc gcgccggagt cgtgttttgt cctcggtgta aaacataagg
     5821 aacccgcctc cgccggcgcc aatcagcttg ccgcccaacg agccgttggc ccgagccagc
     5881 tcgtaatatt cgtcgatcga gcggttgctc attgctcctg aacgcttctt cttgtgttcc
     5941 cagtgcacgt gcatgatatc ggcaaactgc cgcaggttcc cagactccag agcacatcgg
     6001 ctctggtatc ccagttcctt gacgaaatga agattgtcga tcatcgactg gtccgtggag
     6061 cgagtacgcc ggtcctggtc cgcgaggatc tgcgaagcag accgcgtata gccggtaaaa
     6121 aacaacagca gattgtcttc caggttggag agcgtctcgc tgttcagccg caccgggtcg
     6181 acctgcacgc gatcatcggg caggaactcg aaacaggtaa tcccgccgaa ggcggcaatg
     6241 tactggtcct gcttccccac cggctcctgc gcaagatcga tttcgacgtg gcaggcctgc
     6301 tcggcgagtt cttgcttcgg cacgatcgtc ttcttgtgcg tgtgcagcgc ctgcaacagc
     6361 gcagtagtga aggagccgga ggaacccaat cccgtgcccg ccgggatgtc agacatgctc
     6421 gcgatttcca gaccttcggg ggggactccc gtcagcagga gagcttccct gacgagcgga
     6481 tgatggatat cttctattct ctttacacgc tcagtctggg aatacttgat gatcgcttcc
     6541 tcgacgaaag tctgatgcag cgtgatgtaa acgtacttat caatcgccgc agcgatcacg
     6601 aatccgccgt gctggcggta atacgacggc aagtcagtgc cgccgcctcc caatgagatg
     6661 cgcgatggac ttctcgtaat gatcacggtt gccttcccat gcggatctgc tcggcgatgc
     6721 gtagcgccgc ctgagcatca acgatgcccg gtaatggcgt tcgctggagc cggatgtctt
     6781 ccgaaaagtc cgccatctcc gtctcccagg aatcgtcggc ggccggatac tcataaatcg
     6841 tggtttccgg cggacccatt cccggcagca tctggtaatg tgccagccgc tcggtcccgt
     6901 agctaccgcc taagcctgcg atctcgagct tgccgcagcg tccggagtat tcgaacgaga
     6961 acaaattctt ccactcggtc cagctcgcgt gcaggaaggc gacgcggcca ctctccgttt
     7021 gcagcagaaa aaaggcattg tcctccacgg gcatattcca gtagaaggta ggcgcggcac
     7081 cctgaactac cacgaactcg cccagaaacc atcgggcaag gtcgatgagg tggacgccct
     7141 gatcgactgc ttcgcctccg cctgaaatct ccggaactgc gcgccattcc ttttcatagc
     7201 cgggccggcc accgtggccg tagcgggcgc gaacgaacat cggttcaccc agcacgcctg
     7261 tgtcaagaat tgcgcgggcc ttgcggaacg cacggtgata acggtgattg aacccaaccc
     7321 tcaccaacgc tccggttgac cgtgcggcgt cggcgaccgg gtcgagttcg gccgagcaac
     7381 gcgcgcccgg cttttccagc aatacgtgtt tgccagcccc cgccgcccgg gcagccagcg
     7441 gcgccaacac gtcatgagtg gtggcaacaa taacgacatc gaccccgggg ttggcggcgg
     7501 ccgattccgc ctcaatcgtg atttgcgctc caggataaaa gcgggccagg ttctcggcgc
     7561 gttcacgcac ttgatcgcaa catagagcca gcgaccaacc ctgcgggagc gcaagcgctc
     7621 gcttttggcc aatcagaccg cagcctataa tcgcgacgcg cattgtcaac cccaggatag
     7681 cccacgttcg ccgggcacga tgcgccggag cgtatacacg tccgtctttt ggaggtcatc
     7741 gcgatgctcg gcgaagtccg cccacggatc ccaaacggca ctgatgagct taccggtgat
     7801 tccgtcgctc tcggagctgg cgagatatac acatagctcg gcgccgcgct caaggggcgt
     7861 gccgcccgac tccttttgcc gcagcgaggc ttcatagaaa gtttggccta ccttctccgg
     7921 tcccgctgag agcacttctg ctagcaagcg ggtgttgagc gcaccgggtg cgatgctatt
     7981 gacgtcgacg ccggcaccgc gaagctcctc ggccagagtc tctccaaacc gcaccacagc
     8041 cgcctttgag gcggcatagg cgctcaggaa aggcagaggt ttggtggcgc ctccgccgga
     8101 aacaaggagg atcttcccgc gcgtcgcttg ccgaagcaag ggtaggaacg ccctgcagca
     8161 gagcacggtt ccaaggaggt taatggaaat tgcctgagac cagtcggacc atgagacttc
     8221 gtcgactgca cctttgggtc catagacacc cgcgttcgaa accaggatat cgagtttccc
     8281 aaactgcttt ttgatgacct ctgcgagacg gccgacgtcg gcttccgaac tcacatcggc
     8341 acgctccgcg aagacctgcg cgccgggagc ggcagcggcg cgcacacctt ccgcggcgcc
     8401 ttcaagatcg tctgctgtcc gggcgcataa tagcacgctg gcaccttctt ggacgaagcg
     8461 gcgcgcaatg acatagccga atccctggct ggctccagtt acgagagtga ctttgttggc
     8521 gagcttcatg ccgtttgtcc tatatcccct tgcctcatga gcggatctcc ccgaccgact
     8581 gcgaaatagc ggacgcggga atcagccccg agggcctgct gcaggtgcct gcctggatcg
     8641 aacaccactg gctcgcgcat ccgcgtgagc agcaggtcga cagaaatatc ccggaactcg
     8701 ggccagggcg tcgcgattaa caccgcgtcg gcgccgctca gggcgtcggg cgcgttgctg
     8761 cgcagcgaca tggtcgcagc gagttcatcc ggtagtgcgc ggaccgctgg atcaaaggcg
     8821 gaaacggtcg ctccctgctg ggtcagccaa gtcgccgcct caaccgcacc ggagcgccgc
     8881 agagtatcgg ttcccggctt ataagctaac cccagaatcg caatgcgccg gccggccaac
     8941 tcgcccaata cttccggcat gcgccggcac agccagccct tgtgaaaccc attgctctgc
     9001 cacacgcccg ctagtagata cccgttcact ttctgccgct cggcaatctc cagaagaaag
     9061 gcgatgtcac gtgccagtgt gcctccggcg aatgcgctac caggatgcag ataggcgcgt
     9121 gagccgatgc gtacatcggt cttcaatccg cgctcaacct cttcggcatc ggcgcctacc
     9181 ttctcgcaaa gcgccgctat ctcgttaata aaagcaacgg aagtggctag aaaggcattc
     9241 agcgcatgct ttgtcatttc cgccgactct accgacatcc attcaatgcg atcagtaaac
     9301 ggcgaaagca aagcggcaat cgtttcgcga tcctcgtgag ttcggacgcc cgccactacc
     9361 ctgtctggat tcgtgaatac ctcgatcgct ttcccgagcc gcagattctc gggcgaatac
     9421 gcgaagcgca gttcgctgcc gccgtttttg tgccagcacg attccaggcg ggccgtggtg
     9481 ccctccggca actgcgatga gatgagcacc agggatccgg tagcaaggta aggcagcagc
     9541 gacagtgtct gattcacgac gtactcacta tcggctacgt catttttgtc cacgggagtg
     9601 tcgtagcaga cccataggac ttgagccgcg gcgagtccgc tggcgttggc cgtataggcc
     9661 agacggccgt cgcttagcgc cttggccgcc agctcccgaa gacccggttc cgcgacaggc
     9721 atgatgttct gttggagccg ttcaatgatc ggcgcctcgt caaaaccaag gacattgtga
     9781 ccgcgcgagg cgaggcaggc ggcggttacc attcccaagt gccatagtcc ggcgacggcg
     9841 accttcacgc gaccacctct gtgatcgccg gattctgttc cagaaactcg accgtgcgaa
     9901 tgaccgcctg ctggatgttg agctttggac gccagcccaa cgagccgatt gcagtgcagt
     9961 ccagaaagat gaacgggttg tcgcccgccc agccgcgatc gccgccactg tagcgcagtt
    10021 caggcgcgag gcccaggcgc ccgcatatcc agcgaatgga gtcgttcacc tcgcagtact
    10081 cgtccaaccc taggttgaag atgttcactt tcccgggtgc agattcaatg gccagaagca
    10141 tggcgtcaag gcagtcctga acataaagat aagacttccg ctgagagccg tttccaagga
    10201 cctccaagta ctccgggtgg gcgcgcaact gcttgtaaaa atcgagcacg tgcccgtgcg
    10261 tgtaccgttc acccaggatc gaaacaaagc gtaaaataaa agcccgcatg ccgaacgcct
    10321 cacaaaacgc ctcaatgaag ccttcggcgg ctagctttga tgcaccgtag agggaggttt
    10381 ggaccggcat cgaagcgttt tccggcgttg gaaaacacga cgcctcgccg taaacagatc
    10441 cagttgagga gaacgcgata tcgcgcacgc cattaacccg cattgcctcg agcacattca
    10501 cggtggcgat agtgttctgt tcgaggtccc tccgcggatg attggttcca aagcgaacat
    10561 cggcgttggc tgcaaggtgg aaaatgaact cgcatccagc catggcacgg gtcactttct
    10621 cggtgtcgag caggtcgccg gtgatcagcg taaaccgctc ggagcgcgag gcggactcaa
    10681 gaaagcctgc cctgccggtc gaaaagttat cgtagcccac aacttgatgg ttggcagcaa
    10741 gcaatcggtc ggtcagactg ctgccgataa atcccgcgca ccccgagaca aaaaagcgca
    10801 acaaccctcc tcttcctccc ctaaatgcta aatatgcact aataggctga cttttactct
    10861 agcatagcca atggcatcaa agtcggttga gaatagctgc tggtagcctc gccgtttgag
    10921 gcgacccaat gcttcggccg ttcccgccgg gatgcgcagc gacgccgcat ccttgggcgg
    10981 gtagaggtta ccgtcgcgta gaggtgcctc attgaccacc ccatcacggc gagaaaaaca
    11041 gctcgctatc atttcgatga ggcgatcgat tcccacttgg tcgtcttgag ttgcatttcc
    11101 agatgcgaca ccagcggtgc caaacaattg ccttgaacgc ttccgaatgg ggcggaattc
    11161 ggtcggggtg tacgaccgga acgatgacgc agacatccgc caccttggcg gtagatccac
    11221 tatcacgtcc tacgatgccg caaacgctgg cgtccacagg gttggcataa tccagcgctc
    11281 ccactaggtt cggactgacg ttacgctcga cgctaccacc gcctaccgaa aaaactagta
    11341 gtgcgtcctt cgcctgcaga cggctactcg caaccacgct tcgaacacgg tctgccaacc
    11401 ttcaactccg acactttgtc ggtcggcgcg tagctttcga tgcccgccaa ttttcggaaa
    11461 tcgttgaacg catgcgaagc gttgcccgca ctgccgccta cgcccaggat gaacaggcgc
    11521 ccgccgcgct cgcgtaggtc ggctagaagc gctgccatct tttcaatggc gactgcgtcg
    11581 agtttgcggg cgacttcttg tacttcggta agaaattgtg cagaaaaact cgttctgctg
    11641 tctgaatggc tcaccatgag tcagtcaatc tttcattcgg acggaattgc aggatcggat
    11701 gatcttgcag acgccaggcg cggggccgtc tttgcgagtg taacctaatc gtgtggtgct
    11761 ggtcgccggc cgccaggaac tctgcagtgt cgcgcaggcc gtccggcgag ccaatctcgt
    11821 agaggcgctc ctgtacctca aacgctgcca gcttcccgtc cttcagtaga tgggatgagg
    11881 cccgtaggcg caagacgaac ctgttccact acggcgcgcc ggatacgagg aacctagcca
    11941 atcgtttggc gtgagttgcc caggacccgg gcgactccca cgacgcgcga gcccgcgcag
    12001 atcgtggacc gcgcttgcgc aagctttgcg aacgcgcagg agaaaatgta tccgctcaac
    12061 aattccaaga acttaacctc cgccggcaag cttttcatcc agact
//
LOCUS       DATMGX010001016         2431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_18572, whole genome shotgun sequence.
ACCESSION   DATMGX010001016 DATMGX010000000
VERSION     DATMGX010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18572"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..925
                     /locus_tag="VNR65_16715"
     CDS             308..925
                     /locus_tag="VNR65_16715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWJ20360.1"
                     /translation="MHDHVLLRQGLRRLLEDEPDMEVVAEAGNAAEALRKVFEHRPEI
                     VITDAHIFECTADQAEWLILQECPSSKVLFLTTHEGDDFVRKVRADASSCVVRHTSAE
                     ELVEMVRRTHGAPKLGVMERRGAEWPSEELLPRRRVLTGREREVLKLLAEGRTVRSAA
                     DVLGLSMKTVDAHKFNLMRKLGIHNKAELVMWAIQKRVVKLPANF"
     gene            complement(1040..1735)
                     /locus_tag="VNR65_16720"
     CDS             complement(1040..1735)
                     /locus_tag="VNR65_16720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HWJ20361.1"
                     /translation="MLDFTRFEILTFDCYGTLINWEAGLLPALRRILSAHGTRIDDAT
                     LLKLYGDFELRSEAGAFRPYREVLESVVRQFGVELRFTPTAEEARSLPDSLSTWRPWP
                     DTVAALRQLKTRFRLAILSNVDDDLFALTRPQLEVEFDEVITAQQARAYKPSLKLFEL
                     ALNRINAPAHRVLHVGQSIYHDVIPAQALGLATVWVNRPSARPGVGAVKAAEAKPDLI
                     VSSLAELAAAAVE"
     gene            complement(1766..>2431)
                     /locus_tag="VNR65_16725"
     CDS             complement(1766..>2431)
                     /locus_tag="VNR65_16725"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWJ20362.1"
                     /translation="AFMADPEIAAAYRNAPAAKTLHHAYIGGLLDHVVSLFRSCDLMC
                     QNYPQINRDLLLTGAFLHDIGKIHELTYNRSFSYTTKGQLLGHMIIELEMLQAKLALQ
                     PGFPEELKTMIEHLIISHHGQYDFGSPKLPMFPEALMLHYLDDLDSKMEAMRAQFERE
                     SGLETPWTSYNSSLGRPLLNTEKFLAPKKASPPEDPAATPDTELDETPDPTPALPGIE
                     PRR"
BASE COUNT          500 a          580 c          866 g          485 t
ORIGIN      
        1 ctcccgcagt gaagtaacgc agcataaacg ctcgattgta cctgatcgcg agggcggatc
       61 ggggttcgga agagggaatc aaggtccccg tatcctgaag aaaaggaacg ggagggacga
      121 ttttcacgag aaaaatggag cgggcagagg ttacaatgag gtaacgcccg ctccgtctgg
      181 gttcgaaccg aacccgcgct gcatcgcaaa tttctctggc cacgaagaag gagttgcccc
      241 tgtgtgtccc cccgcacaga tacaacgcga aggttttttt ggcgataaag aaattcgttg
      301 tcttctggtg cacgaccacg tcttactgcg ccagggattg cgtcgattgc tggaggacga
      361 accggatatg gaagtggtgg cggaggcagg aaatgccgcc gaagcgctgc gcaaagtatt
      421 cgagcaccgt cccgagatcg tgatcaccga tgcgcacatc ttcgagtgca cggccgatca
      481 agccgagtgg ctgatcctgc aggagtgtcc gtcgagcaag gtgctgttcc tgacgaccca
      541 cgagggcgac gactttgtgc gaaaagtgcg agcggacgcg tccagttgcg tggtacggca
      601 tacttccgcg gaagaactgg tggagatggt gagaaggact cacggcgctc cgaaacttgg
      661 ggtgatggaa agacgcgggg cggaatggcc gtcggaagaa ctgcttccgc gcaggcgggt
      721 gctgacgggg cgagaacgcg aggtattgaa gttgctggcc gagggcagga cggtgcgttc
      781 cgcagccgat gttctgggac tcagcatgaa gacggtggat gcgcacaagt tcaacctgat
      841 gcggaagttg gggatccaca ataaggcgga actggtcatg tgggcgattc agaagagagt
      901 ggtgaagttg ccggcgaatt tctagagggt agctatctgc tacctgtgcc gtccctgcgg
      961 gactggcttt ttccttcggt tcctacccgg cgctggaagc gctgggctac tattttccgc
     1021 ccctctgggg ctcggtcgtc tattcgaccg cggctgcggc aagttctgcg aggctcgaga
     1081 cgatgaggtc gggcttggct tccgcggctt tcacggcgcc gacgccgggg cgggctgaag
     1141 gacggttcac ccatacggtg gcgaggccca gggcctgcgc gggaattacg tcgtggtaga
     1201 tgctttggcc cacgtgcagg acgcgatggg cgggggcgtt gatgcggttc agggccagtt
     1261 cgaacagctt cagtgaaggt ttgtacgccc gggcttgctg tgcggtgatg acttcatcga
     1321 attcaacttc gagttgggga cgcgtgaggg cgaacagatc gtcgtccacg ttggagagaa
     1381 tcgccagacg aaaacgcgtc ttcagctggc ggagtgcggc gacagtgtca ggccagggcc
     1441 tccaggtgga aagagagtca ggtagggagc gtgcttcttc cgcggtggga gtgaaccgaa
     1501 gctcaacgcc gaactgccgg acgacagatt ccagaacctc gcggtaggga cgaaacgcgc
     1561 ccgcctcgga acgcagctca aaatctccgt agagcttgag gagggtggcg tcgtcgatgc
     1621 gtgtcccgtg agcggagaga atgcggcgca gtgcagggag aagtccggct tcccagttga
     1681 tgagtgtgcc gtaacaatcg aaggtcagga tttcgaagcg cgtgaagtcg agcatggttg
     1741 gggccggtcc agccggttga gtactttaac gccgaggctc gataccgggc aaggcgggag
     1801 tgggatcggg agtctcgtcg agttcggtgt cgggagtcgc agcgggatct tccggcggtg
     1861 aagctttttt cggagccaga aatttttcgg tgttcaggag cggacggccg agagaagagt
     1921 tgtagctggt ccagggagtc tcgaggccgg attcgcgctc gaactgggcg cgcatggctt
     1981 ccatcttgga gtcgaggtcg tcgagatagt gcagcatgag ggcttcgggg aacatgggca
     2041 gcttaggaga tccgaagtca tactggccgt gatggctgat gatgaggtgc tcgatcatcg
     2101 tcttgagttc ctcggggaag ccgggttgca gggcgagctt ggcctgcaac atttccagtt
     2161 cgatgatcat gtggccgaga agttggccct tagttgtgta ggagaacgag cggttgtagg
     2221 tgagttcgtg aatcttgccg atgtcgtgca aaaacgcacc ggtgagaagc aagtcgcgat
     2281 tgatctgggg atagttctgg cacatcagat cgcaggaacg gaacagagat acgacgtgat
     2341 cgagcaggcc gccgatgtag gcgtgatgca gggttttggc cgcgggagcg ttgcggtagg
     2401 cggcagcgat ttcagggtcg gccatgaagg c
//
LOCUS       DATMGX010001017         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_139888, whole genome shotgun sequence.
ACCESSION   DATMGX010001017 DATMGX010000000
VERSION     DATMGX010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139888"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VNR65_16730"
     CDS             <1..208
                     /locus_tag="VNR65_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20363.1"
                     /translation="DLRHAFAVHRLVSWYRNGADVQRLLPKLSTYLGHVHIAGTQRYL
                     TLTPELLRQASLRFERYAMEPSHE"
     gene            201..1196
                     /locus_tag="VNR65_16735"
     CDS             201..1196
                     /locus_tag="VNR65_16735"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ20364.1"
                     /translation="MNDEALLGPWVRRFLLEHVVAERNLARNTQHGYRDALCRLIPFV
                     AKQSRKPIDRLAVVDVSAEFVRLFLTDLEVSRHCAIATRNQRLAAIRALAGFVGEHSP
                     IHIEWSGQIRSIPFKKTGKTVVPYLEKPEIDALLAAPDRRTAQGGRDHALLLFLYNTG
                     TRASEAAQMKVADLDLNAASVKITGKGGKQRFCPLWPVTVSELAALIGERASSEAVFL
                     NHRGCPITRFGIHTMVERNVLKALSRSPSMAAKRVSPHSIRHTSATHLLRAGVEINTI
                     RGWLGHVSLETTNIYAEVDFETKAKALAKCDVPDTERIPRRWRDQPGLMDFLRTL"
     gene            complement(1299..>2136)
                     /locus_tag="VNR65_16740"
     CDS             complement(1299..>2136)
                     /locus_tag="VNR65_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HWJ20365.1"
                     /translation="DMVDQKWMIRFVEHRIGDPRIIRLIQKWMKAGVLEDGEFKASER
                     GTGQGSVISPLLANIYLHYAFDLWANRWRQREATGNMIILRYADDTVVGFEHEADARR
                     FLDMMRTRLEEFALTLHPEKTRLIEFGRHAAADREQRGLGKPETFTFLGFTFICSKSR
                     QGHFLIKRKTRQDRMMAKLLELKEEMRRRMHWPISKQGEWLKQVVGGFFNYHAVPTNS
                     RALAAFRHHVMTLWRRTLQRRSQKDQTTWDRIAHLAEEWLPKPRILHPWPNVRFAVKH
                     PR"
BASE COUNT          402 a          691 c          602 g          441 t
ORIGIN      
        1 tgacctgaga cacgccttcg ctgtccatag gctggtttca tggtaccgca atggcgccga
       61 cgtgcaacgc ctgctgccga agctgtcaac ctacctcggc catgtccaca ttgccgggac
      121 gcagcgatac ctcacgctga cgccggaact gctgcgccag gcgagcctgc gctttgaacg
      181 ctacgccatg gagccgtccc atgaatgacg aagctctcct tggcccctgg gttcggcgct
      241 tcctgctcga gcacgtcgtc gccgagcgca atctcgcccg caacacccaa cacggctacc
      301 gcgacgcgct ctgccggctg atccccttcg tggcaaagca atccaggaaa ccgatcgacc
      361 gtcttgcggt cgtcgacgtc tcggctgagt tcgtccggct ctttctcacc gatctcgagg
      421 tctcacgcca ctgcgccatc gccacacgca accagcggct cgccgccatc cgcgccctcg
      481 cgggcttcgt tggcgaacat agcccgatcc atatcgaatg gtccgggcag atccgttcga
      541 tacccttcaa gaaaaccggc aaaacggtcg ttccctatct cgaaaagccg gagatcgacg
      601 cgttgctcgc ggccccggac cggcgaaccg cacaaggtgg tcgcgaccat gcccttctcc
      661 tgttcctcta caacaccgga accagggctt cggaggccgc ccagatgaag gtggcggatc
      721 tggacctgaa cgccgccagc gtcaaaatca ccggaaaagg tgggaagcag cgtttctgtc
      781 cgttgtggcc ggttaccgtc tccgaactcg ccgcgctcat cggcgaaagg gcttcttccg
      841 aggccgtctt cctcaatcac cgcggctgtc caatcactcg atttgggatc cataccatgg
      901 ttgaacgcaa cgttctgaag gccctctccc gatctccgtc aatggcggcg aaacgcgtca
      961 gcccccattc catccgccat accagcgcaa cacaccttct gcgcgccggg gtcgagatca
     1021 acactatccg gggctggctc ggtcatgtct cgctggaaac tacgaatatc tatgccgaag
     1081 tcgacttcga gaccaaagcc aaagcgttgg cgaaatgcga cgtgcccgat accgaaagga
     1141 tacccaggcg ttggcgagac cagcccggcc tgatggactt cctgcgcacg ctgtagccgt
     1201 cttgttatgt ggcgttgaaa aatcgctcag ccgctccagg cgccgatctc cgaggtcagc
     1261 gccacataac tatcggcgcc acattatgca tacggctcct acctcgggtg tttgacggcg
     1321 aagcggacgt ttggccaagg gtgtaggata cggggcttgg ggagccactc ctccgccagg
     1381 tgagcgatcc ggtcccacgt cgtttggtcc ttttggcttc gccgctggag cgtgcgccgc
     1441 cagagagtca tgacgtggtg tcggaaggct gcgagtgccc ggctgttggt cggcacggcg
     1501 tggtagttga agaaacctcc cacgacctgc ttcagccatt ctccctgttt ggagattggc
     1561 cagtgcatgc gtcgtcgcat ttcctccttc agttccagga gcttcgccat catacggtct
     1621 tgccgtgttt tccttttaat caggaagtgg ccctgtcggg acttactaca gatgaaggtg
     1681 aaccccagga aggtgaaggt ttccggtttg cccaacccgc gctgctcgcg gtcggctgcc
     1741 gcatggcggc cgaattcgat caggcgagtt ttctccggat ggagcgtcaa cgcaaactcc
     1801 tccaggcgtg tgcgcatcat gtcgaggaac cgacgggcgt cggcctcgtg ctcgaagccg
     1861 acgacggtat catccgcata gcggaggatg atcatattgc ctgtcgcctc ccgctgtcgc
     1921 cagcggttgg cccagaggtc gaaggcgtag tgcaggtaga tgttggcaag cagcggtgaa
     1981 atcaccgatc cttggcccgt tcccctttca ctggccttga actccccgtc ttcgagcacg
     2041 cccgccttca tccatttctg gatcagacgg atgatgcgcg gatcaccgat tcgatgttca
     2101 acgaaacgaa tcatccattt ctggtcaacc atgtcg
//
LOCUS       DATMGX010001018         2218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_457733, whole genome shotgun sequence.
ACCESSION   DATMGX010001018 DATMGX010000000
VERSION     DATMGX010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457733"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="VNR65_16745"
     CDS             <1..1517
                     /locus_tag="VNR65_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20366.1"
                     /translation="VGREFFTRIGKFEREHQTSLANQWRLQCGGSLPNGPFLEADPIV
                     NSIATMVRDLFPAIMLAIHQGGLSESVLTLKDAVQAQGPALKEFMLHLPQDSLRLIVD
                     PRGIDLDRRATILWSSGHSQVIDRTLLPLDLVLAVVERIRLLGDFATISFDQVVGMIR
                     RVIDDARALGLGEAADVPVIAGLGGLRIVQDPIRISVSDAGPGVQLTNRLEGLLLQHK
                     RRAPFVVEEGVMCQLVFEDECRRLAISDSGLQDVKPSEELRGEIEATARRRQQLLDHF
                     RIGVLLSTDARGARTPVLVAERAIGPLGPRGFVEIIESERVAIFHEPAGRPPGVPYDG
                     WPVISGRVAEDVAMSIRAAKHPGLAVASRRLCLANGTRADPVDRLIDSAVGYEAMFGG
                     QDEKKKYRSVAKAMAKAMAYAIDDRTPTDPVKTAAMIEEISHLRNHHVHGEQDLSRSD
                     LERVARVASGIGLLAFSSIIRDTELLDAPSSFRRRHIVAIRHDFDVMTGKPLTT"
BASE COUNT          489 a          624 c          653 g          452 t
ORIGIN      
        1 aagtgggtcg cgaattcttc acacgaattg gaaaattcga acgagagcac cagacctcct
       61 tggccaacca gtggcgtttg cagtgcggtg gcagccttcc aaatggacca ttcctcgagg
      121 cagacccaat cgttaactcc attgcgacga tggttcgaga tctctttcct gctattatgc
      181 tcgcaataca tcagggcggc ctcagcgaat cggtcctcac gctcaaagat gccgtgcaag
      241 cccaaggtcc ggcactgaaa gaattcatgt tgcatttgcc tcaagacagt cttcgactga
      301 tagtcgatcc acgtggaatc gatttggatc gtcgtgcgac catcctgtgg tcatcaggtc
      361 attcccaggt catcgacaga acgctgctgc cgttggacct tgtgctggca gtcgtcgaaa
      421 ggatccggct actgggtgat tttgcgacga tcagcttcga ccaggttgtc ggcatgatta
      481 ggcgcgttat cgatgacgct cgtgctcttg ggcttggcga ggctgcagat gtgccggtaa
      541 tcgccggcct gggcggcttg cgcattgtcc aggatccgat cagaatctct gtcagcgacg
      601 ccggaccggg tgtgcagttg acaaatcggc ttgaaggact gctgctccag cacaagcgca
      661 gggcaccctt cgtcgtcgaa gagggcgtaa tgtgccaact agtattcgaa gacgaatgcc
      721 ggcgccttgc gatctcggac tctggcctgc aggacgtcaa gccatccgaa gaacttcgtg
      781 gtgagatcga ggctacggcc aggaggcgtc agcaactcct cgaccatttt cgcatcggag
      841 ttctgctctc cacggatgct cgaggtgcac gaacaccggt gcttgtagcg gagcgcgcca
      901 ttggaccact tggcccccgg ggctttgtcg aaataatcga atcggaacgc gtggcgatct
      961 tccatgagcc agctggacga cctccaggtg tcccatacga tggttggcct gtaattagcg
     1021 gcagagtggc cgaagacgtc gctatgtcga ttcgcgccgc gaagcacccc ggattagcgg
     1081 ttgcctcacg acgcctatgc ctcgcgaatg gaacacgcgc tgatccggtc gataggctga
     1141 tcgatagtgc ggtcggttat gaggcaatgt tcgggggcca ggatgaaaag aagaagtata
     1201 gaagtgtggc caaagccatg gcgaaagcca tggcgtatgc aattgacgac aggacgccga
     1261 ccgatccagt caagacagcc gcaatgattg aagaaatcag ccaccttcgc aatcatcacg
     1321 ttcatggaga acaggatctc agtcgtagcg acctcgaaag ggtcgctcgt gtagcctcgg
     1381 gtatcggctt gctcgccttc tcgtcgatta ttcgtgatac cgagctgtta gacgcgccct
     1441 cgtcatttcg tcggcggcac attgtggcca ttaggcacga ctttgacgtt atgacgggca
     1501 aaccgcttac aacctaattg aggagcgggg gcgggtttgg gaatcctgat atgctgttct
     1561 gccggtgcag tgcagggcga tgcgaatttt gcgccgtgca ataacggaat gaggcctcgg
     1621 tcagtttgtg gtgggccaac ggcggctagc ggacaagccc tttttgcggc atgtattcgg
     1681 cgccgcgtgt agctcgcgta gatgtggttg gagcgctaga gcccgacctc cttgcaactg
     1741 gcttcacggt ttcgactacg ttgtcgcgca tgggccacca caacttgacc tcacctagaa
     1801 gggccgtagt gacggcgttc aaggcggcgg ccgagcgcac cgaaacggcg accagccgag
     1861 cggatcaagc gctccgatga caccgcacta cgcgccgcga tgcaacagac cgaatacgga
     1921 ttcgaatcac actctccgaa gcttgccggc tccaacgtca gcgcggccag gctacgaacc
     1981 gacctggcac tgggctcggc atgagctgaa acagacccga gcagaacgag acacccttcg
     2041 ccgcatggaa gaacgcgtac tgggcgacga ggtcagggaa accgacccac gcgaactcgt
     2101 ccaccgcatt accgaacttg aaaaccttct ccgcgacagc caaaccagcg atcgcaaaag
     2161 ctcactgggc ataactgatc gtgaaaatcg gtgcgccgac ctcaccgagc aactagtg
//
LOCUS       DATMGX010001019         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_438201, whole genome shotgun sequence.
ACCESSION   DATMGX010001019 DATMGX010000000
VERSION     DATMGX010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438201"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VNR65_16750"
     CDS             complement(<1..381)
                     /locus_tag="VNR65_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20367.1"
                     /translation="MSIKSIVDRFQRAPSVHFSGSRDPIDVLAAEIDHLEKRVGAALR
                     HRSFEIASALAPTTTEPDNTVVALRLLIGALTPLDDAKSWLLLAAIHGRLPDGAQVKA
                     TARTAKLDGTAAAVVATVTSWIQDF"
     gene            complement(359..1930)
                     /locus_tag="VNR65_16755"
     CDS             complement(359..1930)
                     /locus_tag="VNR65_16755"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWJ20368.1"
                     /translation="MTGVGRSLLEVEGAPTAKSEDALAARLRAIAPLLLDAPLPDADR
                     DPASDAANTLDLLVEFVAAKPSSSRIWLLLTSLCAAYPTRTEIDRIRRLFELLDHRNA
                     VISLLDISLSVASARGGPETDIHIIEGGVIVDVDHSAKFDLNTGIQRVTRCLLPRWND
                     QHEIVPAAWTANAGALRSLTPMELERVVSWSSRKPATRADDHNSSRTAPRLLVPWRSV
                     VVMAEVPPGNVSDSLAAIGDYSGSRLVGIAYDSIPVVSADMVPAADTSKFVRYLTCLK
                     FASRMAGISSSAASEIAGFVQALSTQGLPGPVVVEVPLASPAADFSNVRTPDTNVPNV
                     LVVGSHEPRKNHLAILHGAEILWREGAKFSLSFIGGSGWGDEFPRRAAELVRADRPIT
                     VRRAVSESELEASYATASFTVFPSLHEGYGLPVAESLAHGVPVITSDFGSTAEIGAHG
                     GALLVNPHDDDALTSAMRTLLSDGQTQRRLRQEIRSRPERTWQNYSDELWELLIEPEL
                     EMLKGDIDGVNKVDR"
     gene            complement(1927..>2264)
                     /locus_tag="VNR65_16760"
     CDS             complement(1927..>2264)
                     /locus_tag="VNR65_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20369.1"
                     /translation="ATDLTADRDRLAAELDQLRHEMLEHDAKNAAAILDWRRSAIKAW
                     AVAVRTDRVRQIESLNRQIASHLNHIVHVDDELGRAKDEVEAMKHTVSWRLTKPLRRI
                     RGVSARSKP"
BASE COUNT          452 a          651 c          685 g          476 t
ORIGIN      
        1 gaagtcctgt atccagcttg tgacagtggc tacgacagcc gcggccgtgc cgtcgagttt
       61 ggctgtgcgt gcggtggcct tgacttgcgc gccatctggc aaacggccat gtattgcggc
      121 gagcaacagc caggatttgg cgtcgtccag cggtgtcagg gcgccgatca ggagccgaag
      181 cgcgacgacg gtgttgtcgg gctctgttgt cgttggcgcc aacgcggaag cgatttcgaa
      241 ggagcgatgg cggagtgcag cgccgactct cttttcgaga tgatcgattt ctgctgccag
      301 gacgtcgatc ggatcacgag atccactgaa atggacagac ggcgcccgct gaaaccgatc
      361 aacgatcgac tttattgaca ccgtcgatgt ctcctttcag catttctagt tccggttcga
      421 tcagaagctc ccatagctca tccgaataat tctgccacgt gcgttccggt cgggaccgga
      481 tttcttggcg aagacgacgc tgagtttgac cgtcgctgag cagagtgcgc atggcagatg
      541 tcagcgcatc gtcgtcgtgc ggattgacga gtaaagcccc gccgtgagcg ccgatctccg
      601 ccgtcgatcc gaaatcagat gtgatcacag ggacgccatg agcaagtgat tcagcaaccg
      661 gaagaccgta tccctcgtgc aaggacggga agactgtgaa tgaggcagtt gcatacgagg
      721 cttcgagttc tgattcagaa actgctcgtc ggaccgttat gggtcggtcc gcacgcacta
      781 gttcggcggc ccgacgcgga aattcgtcgc cccagccgct accgccaatg aatgacagtg
      841 agaatttcgc gccttctcgc cacagaatct cggctccgtg cagaatagct aggtgattct
      901 tgcgcggttc atggcttcca acgaccagga cattgggcac atttgtatcg ggcgtgcgaa
      961 cgttgctgaa atcggcggcc ggtgacgcca gaggaacctc cacgacaacg ggaccgggta
     1021 acccttgagt actcaatgcc tgtacgaaac cagcgatctc ggacgcggcc gagctggata
     1081 ttccagccat tcgactcgcg aacttcaaac aggtgaggta gcgaacgaac ttgctggtgt
     1141 ccgcggcggg caccatatcg gcggaaacga ccgggatgga atcataggca atgccgacca
     1201 gccggctccc cgagtagtca ccgatcgctg ccaaactgtc agaaacgttg ccgggtggca
     1261 cttccgccat cacaaccaca cttcgccagg gcacaagcaa gcggggcgct gtgcgggagc
     1321 tgttgtgatc gtcggccctt gtcgccggtt tccgactgga ccaggaaacg accctttcga
     1381 gttccatcgg cgtgaggctt ctgagtgctc ctgcgtttgc tgtccacgcc gccggtacga
     1441 tctcgtgctg gtcattccat cttggtagca ggcatcgggt gacacgttga attccggtat
     1501 tcaagtcgaa tttggcgctg tggtccacat cgacgatgac tccaccttcg ataatgtgga
     1561 tgtcagtctc gggtccgccc cgcgcggacg ctacggacaa gctgatgtct agcaacgata
     1621 tgaccgcatt gcggtgatcg aggagctcga acaggcgacg gatgcggtcg atttccgtcc
     1681 gcgtcggata agcagcacaa agcgaggtca ggaggagcca aatgcgacta ctggacggtt
     1741 tagccgcgac gaattcaacc agtaggtcga gtgtatttgc agcatccgac gccggatccc
     1801 gatcggcgtc tggcagggga gcatccaaca atagtggagc gattgctcgt agtcgcgcgg
     1861 ccagtgcgtc ttccgacttc gctgtcggtg ctccctccac ttccaacagt gagcgaccga
     1921 ccccggtcac ggcttgctcc ttgcgctgac tccgcgaatt cgccttagtg gtttggtcaa
     1981 tcgccaggag accgtgtgct tcatcgcctc gacctcatcc ttcgctcggc ccagttcgtc
     2041 gtcgacatgc acgatatgat tgaggtgaga tgcgatctga cgattgagtg actcgatctg
     2101 gcgcacacgg tctgtccgca ctgccaccgc ccaggcctta atggcagatc gccgccaatc
     2161 gagaattgcg gcagcgttct tcgcgtcatg ctcgagcatc tcgtgcctga gctgatcgag
     2221 ttcggcagcg agccggtcac gatccgcagt gaggtcggtg gcgg
//
LOCUS       DATMGX010001020         2022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_438661, whole genome shotgun sequence.
ACCESSION   DATMGX010001020 DATMGX010000000
VERSION     DATMGX010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438661"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VNR65_16765"
     CDS             complement(<1..264)
                     /locus_tag="VNR65_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20370.1"
                     /translation="MAKVQRVRRIVRLSLLLAWGMTMAACQLGLASAGYEQTQTQAQA
                     QGASAAAPQSGPGVVIENEPQMPDGYPHRHYELRLHARGGVSVL"
     gene            491..907
                     /locus_tag="VNR65_16770"
     CDS             491..907
                     /locus_tag="VNR65_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20371.1"
                     /translation="MELLLNLAWLLLALPAYGLWRASRTPQASRKFSSLQCLLALGCL
                     LVVLFPVVSATDDLRAMRAEMEESPSSKRTIRQASSHKASPWKLQTPPALVSTSNAVL
                     IANNEDWHQLPAPDLSIPPAHALANAGRAPPDSLPA"
     gene            995..>2022
                     /locus_tag="VNR65_16775"
     CDS             995..>2022
                     /locus_tag="VNR65_16775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWJ20372.1"
                     /translation="MHCKLLGLAFAFGAASLLAQPAPAPAPQIWTWDAVKDRFELNNT
                     SLQASRLNIDELKAQEITAHLRPNPDFTLSADGTQIAPSHGVWTPFAGTFVSPGVSYL
                     FERRNKRGLRYEAAKQGTAIGVAQQSDSERNLVFNLRSAFVGVLQAKAVLRLAQDNLD
                     SYDNILKVSRDRFSAGDIARIDLDRLELQRLQFESDLQTALVNLRTTKINLLALLDDR
                     RPVDSFDLDGRFDFSEDLASLDDYRKDALDARPDLRAAVLSVKQAETNYQLAEANGST
                     DPTIGVWFTHNGSFNNPDALNTIGASVSIPLRFFDSNQGEKLRTKIDVKRNEKLREGV
                     ETQVYADV"
BASE COUNT          407 a          744 c          482 g          389 t
ORIGIN      
        1 cagcaccgac acgcccccgc gggcatgcag gcgaagctca taatgtcgat gcggataccc
       61 atccggcatc tggggttcgt tctcaatcac cacgcccggc ccgctctgtg gcgctgccgc
      121 gctcgcgccc tgagcctgag cttgagtctg agtctgctcg taacccgccg atgccaaccc
      181 caattgacat gccgccatcg tcatccccca tgccaacagg agactcagcc ttacaattcg
      241 ccgcaccctt tgcaccttcg ccatttcccc accctaagcc gtacgaatca gctcgatctg
      301 tggccctaaa tcccgattcc cctactctcc cacacttccc ccacttccgt ataagtaata
      361 agaatgggcc tccaactcga tcttgcgccg ccggccccct cgcaacacgg tagtcacatt
      421 gacttagatt tgcactttct gaaaaactac ccactctggg gttctttctt cgagcccaag
      481 cttcgttttg atggaactgc tgctcaatct cgcgtggctg ctcttggcgc tcccggccta
      541 tggcctgtgg cgcgcgtcca gaactccgca agctagccgc aagttcagtt ccctgcagtg
      601 ccttctggcg ctgggatgct tactcgtcgt cctcttcccc gtggtttcgg ccaccgacga
      661 tctccgcgcc atgcgcgccg agatggaaga gtctccttcc agcaagcgca ccatccgtca
      721 ggccagcagc cataaagcat caccctggaa gttgcagact ccgcccgcac tggtctcgac
      781 ctcgaacgcc gtgttgattg cgaataacga ggactggcac caattgccgg ctccggatct
      841 gtccataccg cccgcgcatg ccctcgcgaa cgcgggccgc gctccccctg attctctccc
      901 cgcgtaatcc cccgacccgc caatcgtttc atcgaagaaa aacccgcagt gtagggacga
      961 atccttacac cgcggatgcc cgatggagtc tctgatgcat tgcaaacttc ttggtctagc
     1021 gttcgccttc ggcgctgcca gcctgcttgc ccagcctgcg cctgcgcccg cgccccaaat
     1081 ctggacctgg gacgcggtca aagaccgctt cgaactgaac aacacatccc tccaagccag
     1141 ccggttgaat atcgacgaac tcaaggcaca ggaaatcacc gcccatctcc gccccaatcc
     1201 cgacttcacg ctgtccgccg acggtaccca gatcgctccc agccacggtg tgtggacccc
     1261 attcgccggc accttcgtct cccccggcgt cagctatctc ttcgagcggc gcaacaagcg
     1321 cggactccgc tacgaagccg ccaagcaagg caccgccatc ggcgtcgccc agcagtccga
     1381 ctcggaacgc aatctcgtct tcaacttgcg cagcgcattc gttggcgtcc tgcaagccaa
     1441 agctgtgttg cgcctcgcgc aggataatct cgactcctac gacaatatcc tcaaagtcag
     1501 ccgcgaccgc ttctcggccg gagatatcgc ccgcatcgat ctcgatcgcc tcgaactgca
     1561 acgcctgcaa ttcgagtctg acctgcaaac cgcactggtc aatctgcgca ccaccaagat
     1621 caacctgctc gccctgctcg atgatcgccg tccggtcgac agcttcgacc tcgacggacg
     1681 cttcgacttc agcgaggatc tcgcttcact cgatgactat cgcaaagacg cactcgacgc
     1741 ccgccccgac cttcgcgcag ccgtgctctc ggtgaagcag gccgaaacca actaccaact
     1801 cgccgaggcc aacggatcca ccgatcccac catcggcgtc tggttcacgc acaacggatc
     1861 attcaacaat cctgatgccc tgaacactat cggcgccagc gtcagcatcc cgctgcgctt
     1921 cttcgacagc aaccaaggcg aaaagctgcg cacgaagatc gacgtaaagc gcaacgagaa
     1981 gctgcgcgaa ggcgtcgaga ctcaggtcta cgccgacgtc ga
//
LOCUS       DATMGX010001021         4621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_240577, whole genome shotgun sequence.
ACCESSION   DATMGX010001021 DATMGX010000000
VERSION     DATMGX010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240577"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1392
                     /gene="lpxB"
                     /locus_tag="VNR65_16780"
     CDS             196..1392
                     /gene="lpxB"
                     /locus_tag="VNR65_16780"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HWJ20373.1"
                     /translation="MAQTKRIFIVAGEHSGDVLGGKLIEALKETAGANAFTFAGVGGE
                     HMEAAGLPSIFPLSDVAVMGPAAILARLPKLVRRVYRTVDAALAFNPDAVVIIDSPEF
                     THPIAKRIRQRRPATPIIDYVSPSVWAWRPGRARKMRPYVDHLLALLPFEPAAHAQLG
                     GPACSYVGHPLIERTPWIDGLDTEALRSRLSLRSDLPVLVVLPGSRPSEVSRLMAPFG
                     ETVAALHQGSGPFEIIIPAVPSVRALIEQALPAWPQQPHLVEGEIDKFTAFKLAHAAL
                     AASGTVTLELGVTGTPMVVAYRVDPVAARLRFLLKVHSVVLANLVLGANAFPEYIQED
                     CTPTKLSSALAPLLIDTPERGAQLAALATIRQKMFLLEGTPSGKAADIVLSILQNARA
                     STRAAA"
     gene            complement(1562..2668)
                     /locus_tag="VNR65_16785"
     CDS             complement(1562..2668)
                     /locus_tag="VNR65_16785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ20374.1"
                     /translation="MNEHLSWELPARRSPRRGLRSRHFLARFISVRSFLDFEKILGRS
                     MNARTRVCVGVCTIQRPAMLERCLQSLAAQESPDDLDLHILVVDNDATPSAKPIVGKV
                     ASNSRFPIHYHHEPRRGIPMARNRVLEEALSLGAEWLVFIDDDQTARPSWVIGHLLVA
                     CRDKADAVQPRVIVTYPEPAPFWCTPTPQDLAEGGVDGPVENRRRKTVATNGVMMSTR
                     LIRPDGMGLRFNESLALCGGEDGEFFETANRLGAHLVTSCLPVVTEESHRSRYSYLRY
                     SLKGFAYGGSYVTRYRLKYGRWAAVRRYSAVSAVRALRGAGQLLISPVFVPFAMRRFK
                     FTALEGGRNIFFAAGTIGGLFSLQYEFYRQIDGD"
     gene            2747..3871
                     /locus_tag="VNR65_16790"
     CDS             2747..3871
                     /locus_tag="VNR65_16790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ20375.1"
                     /translation="MQSECLIVTQCFYPDVGGIEGLMTGLADHCASSGRSVTVLAERI
                     RHKLLHPVTPKPYDIIRFAGPRPWRRWRKRACLDGIVKRRGENIAGIFVDSWKSVEAL
                     PAHLKMPVTMLAHGMEYPTDATPAKKRRICSALARCSAIVANSRYTAGLVLPYLDGNA
                     QKLQMIHPPIAPMPEPKADTVARLRDRIGCRHPVIATVARLEPRKGIDAVIRALPDVL
                     TRHAGCIYAIAGTGEDRERLEQLTVDLNVREAVLFLGRVSSSEKAALLAIADVFAMPV
                     RREGRSVEGFGLSYSEAGWFSVPSLAGRDGGAGDAVVDGETGLLCDGASQSEITSSLI
                     SLLDDEALRERLGAAAQVRVKNELIWPIVIDRYLDSFRGC"
     gene            complement(3946..4560)
                     /locus_tag="VNR65_16795"
     CDS             complement(3946..4560)
                     /locus_tag="VNR65_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20376.1"
                     /translation="MFGVGLSAEVFSGVAVAMSMYSLWRTSLKRADLRVFVSPLIRYA
                     SPYQNSVFEVFEIPLTVINEGAQTGTILSFDLEVTSSQNGTSKLFYSAGLGPWSLARV
                     KGEGLTPFTPVSLTGRSSQSETILFYARNDSRVMQIVEAPAQYRFAITPLTAQRRSLL
                     RRNPKPLMFEMALPYLDHRAFTSGGGTLPLHKASWSGEKPAVAR"
BASE COUNT          934 a         1345 c         1397 g          945 t
ORIGIN      
        1 ctagtacgcc ccgcgggaaa tcccgcccca ttttgcggca agctcgcacg ggatttcccg
       61 cggataaggc gtactagcaa cacttcgatt ctagtacagc ttatcggagg cgagttcgct
      121 tgagagcatg ccgcggagtg agtgcgaact tgcctctggc tatactagcc tgacgaggca
      181 aagacggcgg gggccatggc tcagacaaaa cgcatcttca ttgtggcagg cgagcattcc
      241 ggcgacgtgc tcggcggtaa gctgatagag gcgctgaagg agacggctgg cgcaaacgct
      301 tttaccttcg ccggcgtcgg cggggagcac atggaggcgg ctgggcttcc atccatcttc
      361 ccgctctccg acgtggcggt catgggacct gccgccattc tcgcccgcct gccgaagctt
      421 gtgcggcggg tttaccgtac cgtggatgcg gcgcttgcct tcaatccgga tgcggttgtt
      481 atcatcgaca gccccgagtt tacgcacccc attgcaaaac gcatccggca gcgccgaccg
      541 gcgacaccga tcatcgacta tgtaagcccc agcgtctggg cttggcggcc ggggcgcgcg
      601 cgcaagatgc ggccctatgt cgatcacctg ctcgcgctcc tgccgttcga gccggcggcg
      661 catgcgcaac ttggcggtcc cgcctgcagc tatgttggcc atccactcat cgaacggaca
      721 ccctggatcg acgggcttga tactgaggct ctacgatccc ggcttagttt aaggtctgac
      781 ctccccgtcc ttgtcgtgct gccgggcagc cgtccgtcag aggtgtcgcg cctgatggcg
      841 ccgttcgggg aaacggtcgc ggctttgcac caagggtctg gccctttcga gatcatcata
      901 ccggcggtgc cctctgtgcg cgcgctgatc gagcaggcgc ttccagcctg gccgcaacag
      961 cctcatctcg tcgaaggtga aatcgacaag ttcaccgcgt tcaaactggc gcatgccgcg
     1021 ctggccgcct ccgggactgt aacactcgaa ctcggtgtca cgggcacacc catggtggtg
     1081 gcttatcggg tcgatcctgt cgcggcgcgc ctgcggttct tgttaaaggt ccattcagtc
     1141 gtgctggcaa accttgtgct cggcgcaaac gcattcccgg agtacatcca ggaagattgc
     1201 acccccacga aactcagctc cgcccttgct cctcttctga tcgacacgcc tgagcgaggt
     1261 gcccaactgg cggcgctcgc aacaatccgt cagaagatgt tcctgctgga aggaacgccg
     1321 agcggcaagg cggcggacat cgtcctttca atcctccaaa acgcccgagc ttcgacccgc
     1381 gcggcggcgt aaccatcgtc aaattcatac tctcggcgtg agtcacggtg ccatcgcgca
     1441 tggcgtcagt ggtggcaaaa gtacttacat gcctacttat gatcttttta cgaaattaac
     1501 acacactcac tcgcccgaag cggagggaca tgaagctggg gtcaggttca ccgcgcgtgg
     1561 atcaatcgcc gtcgatttga cggtaaaact cgtattgcaa agagaacagg ccgccgattg
     1621 tacctgccgc aaagaaaata ttgcgcccgc cttcaagggc ggtgaatttg aaacggcgca
     1681 tggcgaaggg aacgaagacg ggcgaaatca aaagctgacc ggccccccga agcgcccgaa
     1741 cggccgagac cgcactgtat cgcctcacag ccgcccagcg cccatacttg agccggtacc
     1801 gcgtcacgta cgatcctcca taagcgaacc cctttaagga gtagcgcaga tatgagtagc
     1861 gcgagcggtg cgattcctca gtcacaacag gcaagcacga ggtgacgagg tgcgccccaa
     1921 ggcggttggc ggtttcgaag aattcgccat cctccccgcc gcatagggca aggctctcgt
     1981 tgaaccgcag gcccatcccg tctgggcgga tcagtcttgt ggacatcatc acgccattgg
     2041 ttgcaaccgt cttacgcctg cggttctcaa caggtccgtc aacgccgccc tcggcgaggt
     2101 cttgtggcgt tggggtgcac caaaagggag cgggttcggg gtaggtgacg atcacgcgag
     2161 gctgtacggc gtcggccttg tcgcggcagg cgaccaaaag gtgtcctatt acccacgatg
     2221 ggcgcgcggt ctggtcatca tcgatgaaga caagccattc ggcgcccagc gaaagcgcct
     2281 cctcaagcac tcggttcctc gccatgggaa taccgcgcct tggctcgtga tggtagtgga
     2341 tcggaaatcg ggagttcgac gcgaccttcc caactatggg tttggctgaa ggggtcgcgt
     2401 cattatcgac aacaaggata tgcaggtcta aatcgtccgg gctctcttgg gcggcgagag
     2461 attgaaggca cctttcgagc atcgcggggc gttgaattgt gcaaactcct acgcacaccc
     2521 gtgttctcgc gttcatggat cgacccagaa ttttctcgaa atctagaaat gacctcacac
     2581 ttatgaatct ggctaaaaaa tgtcgggaac gcagtcctct acgaggcgag cggcgcgctg
     2641 ggagttccca agaaaggtgt tcgttcatct gttgatgcgg aagccgccgc cgcttatgac
     2701 tgcctaaact cagcgcggtg ggttagaaaa ggcgaggcat ttcggcatgc aatctgaatg
     2761 tttaatcgtc acccaatgct tctaccctga tgtgggcggc attgaagggc tgatgaccgg
     2821 gctcgcagat cactgtgcgt cttcggggcg ttctgtcact gttcttgcgg agcgcatccg
     2881 tcacaagctg ctccaccccg taacacccaa gccatatgac attatccgct tcgccggtcc
     2941 ccgtccgtgg cggcgttggc ggaagcgggc ttgtctcgat ggcatagtga aacgccgggg
     3001 cgagaacatc gctggcattt tcgtcgattc atggaagagt gttgaagccc tcccggctca
     3061 cctcaagatg cctgtcacga tgttggcgca tggcatggag tatccgacag acgcgacgcc
     3121 ggccaagaag cggcgtatct gttcagcgct tgcacgatgc agcgccatag tcgccaattc
     3181 gcggtacacg gctggtctgg ttcttcccta tctggatggg aacgcccaaa aacttcaaat
     3241 gatacatccg cccatcgcgc cgatgcctga gcctaaggcc gacacggtag cccgtttgcg
     3301 agatcgaatc gggtgccggc acccggtgat agcgacggtg gcccggctgg agccgcgcaa
     3361 gggtatcgat gccgtgatcc gcgcgctgcc ggatgtgctg acccgccatg ccggctgcat
     3421 ttacgctatt gccggtacgg gcgaggaccg cgagcggctc gaacagctga ctgtggatct
     3481 gaatgttcgc gaagctgtcc ttttcctggg tcgggtaagc agttcggaaa aagcggcatt
     3541 gctggccatt gcggatgttt ttgccatgcc ggtgcgccgc gaaggccgat cggttgaagg
     3601 ttttgggctg agttattccg aagcgggatg gtttagcgtt ccttcgctgg ccggccgcga
     3661 cggtggtgca ggcgacgccg tggtggatgg tgaaacgggg cttctctgcg acggtgcgag
     3721 ccagagtgag atcacgagca gcttgatctc gctcctggat gacgaggcgc tcagagagag
     3781 gctgggagcc gcggctcagg tcagggtcaa aaatgagctt atttggccga tcgtgatcga
     3841 tcgttatctg gatagcttta gaggatgctg agcaggcatt tctgggttac ctgaaagcct
     3901 gacttacaag cgcgcagggc aggccgctga caatcgcttg cagcgttagc gggcgacggc
     3961 cggtttctcc ccgctccaac tcgctttatg cagcggaagg gtcccgcctc cgctcgtaaa
     4021 agcgcgatga tcgagatacg gcagggccat ctcaaacatg agcggtttcg gatttcggcg
     4081 cagaagactg cggcgctgag ctgtcaaagg tgtaatggca aagcggtatt gagcaggcgc
     4141 ctctacaatc tgcatcaccc gggaatcgtt tctggcgtaa aaaaggatcg tctcggattg
     4201 ggaggaacgt ccagtgagcg atacaggcgt aaacggcgtc aagccctcgc ctttgactct
     4261 ggcgagactc catgggccga gccccgcact gtagaagagt ttcgatgtgc cgttctggga
     4321 gctggtaact tcaagatcga aagacaggat ggtccctgtc tgtgctccct cattgatgac
     4381 agtcagcgga atttcgaaaa cttcgaaaac gctgttctga taaggggacg cgtatcggat
     4441 gagcggcgac acgaaaacgc gcaaatcggc ccgcttgagc gatgtgcgcc atagactgta
     4501 catcgacatc gctactgcaa cgccggagaa gacttccgcc gaaaggccaa caccaaacac
     4561 tggttatccc tctttactgc cccgctccac ctttcccgat cggaggcgcc ttagtcaacg
     4621 a
//
LOCUS       DATMGX010001022         2859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_200833, whole genome shotgun sequence.
ACCESSION   DATMGX010001022 DATMGX010000000
VERSION     DATMGX010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200833"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VNR65_16800"
     CDS             <1..1242
                     /locus_tag="VNR65_16800"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWJ20377.1"
                     /translation="SAHLEPEKGAAGQSVSSGITAPQEEPVLNERSATAKPSEQIGVA
                     PDAQTAATAEPAPPSEAAIEKPKPQAQEPKAEAFAEVASTSRSPESPVHEPAQPSAGQ
                     ARQPALQKPEATRAERDAPQTPLAEVLAKRPPDVSSTEPAPPPAERPRGLAMAKAQAK
                     QSDATMAAPAQPPEKPASAKAPDQRVAKAEPVAPPLPEASAASPAGLKFKDCDFCPPL
                     VVVPNGEFQMGSSEPHEKPAHKVKLPLPFAIGQYEVTYAEWDRCIEAGACRYRPEHQG
                     SPNDAVGNLSWDDANAYLKWMSEQTGQIYRLPSEAEWEYAARAGSVKAAKNKQPMADE
                     TSKANSFGLYGTEGNRAEWVEDCWNESYQGAPADGSAWVKGNCSLRGLRGGPFGSSSR
                     FRYDSDVRYEANGFRVLRELH"
     gene            1525..>2859
                     /locus_tag="VNR65_16805"
     CDS             1525..>2859
                     /locus_tag="VNR65_16805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWJ20378.1"
                     /translation="MESGQALVPGSLISESTRIVDLVFQDPFYVVYSAEATASGATIG
                     ITEYFPADLVARAPDGEVLLRSPELQDLFNVGRDRFMAEARALSALRHPSLIRYDSIL
                     SDHGTAFALHAAEEGQSVTSIVKSSKQPPPQDELDASVKQLVSALELLHSRDLIHANI
                     NPDSVLLRPEPLLIRFGATRNFLAAKMRKVNLAVTPGYSAPELHFADAKAHGPLCDIF
                     SLAAVLYYIVTGRHPINVIARGLGHTMPPAAAVPFQKFRPEFLEAIDRGLSLDPDRRP
                     QTIKAFGQMLLEIPEKIAPKNRQPALQMAASLAEGPPPSALPPNGPPSPVTIPLPKSA
                     VPDPDGENDDDDDDDLDEHRDFRSGRRGLGILVLAVLLALLIPTGLWLLESQSGKVPE
                     QSAHLEPEKGAASQSVSSPTEPAPPPAERPRGLAMAKAQAKQSDATMAAPAQP"
BASE COUNT          628 a          879 c          831 g          521 t
ORIGIN      
        1 tcggctcacc ttgaacccga aaaaggcgcc gcgggccaaa gcgtgagttc cggcatcacc
       61 gctccacaag aggagcccgt gctcaacgaa cgctctgcaa cggccaagcc atcggaacaa
      121 atcggtgtcg cgccggatgc acaaaccgca gcgacagctg aacccgcacc tccgagtgag
      181 gctgcgattg aaaagccaaa gcctcaagca caggaaccca aagcagaggc tttcgctgaa
      241 gtggcctcaa ccagccgttc accggaaagt ccggtgcatg aacctgccca accttcggcg
      301 gggcaggccc gccagcctgc gcttcaaaag ccagaggcga ccagagctga gagggacgct
      361 cctcaaactc ccctggccga agtcctcgcg aagcgtccgc cggacgtatc gtcgaccgaa
      421 cctgcaccgc caccggcgga acggcctcgg gggcttgcaa tggcaaaggc gcaggcaaag
      481 caatctgacg ccactatggc agcgccagcg cagccacctg agaagccggc gtccgcgaag
      541 gcgcccgacc agcgggtggc gaaggcagag cctgtcgcgc cgccgctgcc ggaggcctcc
      601 gcagcatcgc cagcagggct caaattcaag gattgcgact tctgcccccc gctcgtggtg
      661 gttccaaacg gcgagttcca gatgggatcg agcgagcccc atgagaagcc cgcgcataag
      721 gtgaagcttc ccctgccctt cgccatcggc cagtacgaag tcacctatgc ggaatgggac
      781 cgctgcatcg aggcgggcgc ctgccgctac cggccggagc atcaaggctc accgaacgac
      841 gccgtcggca atctgagctg ggacgacgct aatgcgtatc tgaaatggat gtcggaacag
      901 accggccaga tttaccgcct gccgagcgaa gccgaatggg agtatgcggc ccgcgctggc
      961 tccgtcaaag ccgcgaagaa caagcaaccc atggccgacg aaacttcaaa ggcgaattcc
     1021 ttcggacttt acggtacgga ggggaatagg gcggagtggg tcgaggattg ctggaacgag
     1081 tcctatcaag gagcgcctgc ggacggttcg gcatgggtga aaggcaattg cagcctgcgc
     1141 ggtttgcgcg gcgggccatt cggcagcagc tcgcgctttc gctatgacag cgatgtccgt
     1201 tacgaagcaa acggcttccg cgtcctcagg gaactgcact agtggtccga ttctgacatt
     1261 tgcatctgct tgcatcacaa tcgagagcaa atgccggaat tataagtatt atcagtaacc
     1321 tcttgatccg ctgggacttt tggatttgac atttgagcac gaactcacgg ccggcgggac
     1381 gcaatgtcaa attcgctctc ctagtcgcag gacagcggcg cttcgacgga cgcaacggcc
     1441 cagcatccga attaaaatca actgcagagc tagcggcggg ccttcaaggc caaggataac
     1501 aaagacacct tgaaggatgg cactatggaa agcgggcagg cccttgttcc aggaagcttg
     1561 atttcggaaa gtacaagaat tgtcgatctt gtctttcagg atccgtttta tgtggtgtat
     1621 tccgctgaag cgactgcatc gggcgcaacg attggaataa ccgagtattt ccccgccgat
     1681 ctcgtcgcga gagcccccga tggcgaggtg ctgttgcggt ccccggagtt gcaggatctc
     1741 ttcaatgtcg ggcgggatcg cttcatggcc gaggcgaggg ctctatcggc actgcgccac
     1801 cccagcctca ttcgttatga cagcatctta tccgatcacg gcaccgcttt tgccttgcac
     1861 gccgccgaag aggggcaatc tgtcaccagc atcgtcaaat cttccaaaca gccgccgccc
     1921 caggacgaac ttgatgcgag cgtgaagcag ttggtatcgg cgcttgagct cttgcactcc
     1981 cgcgatctga tccacgccaa tatcaacccg gatagtgttc tcttgcgacc ggaaccgctt
     2041 ctgatccgct tcggagccac aagaaatttc ctggccgcaa agatgcgcaa agtcaatctg
     2101 gctgtaacgc cagggtattc cgcgccagag cttcatttcg ccgacgcaaa ggcgcatggg
     2161 cctctttgtg atattttttc actggccgcc gttctctatt atatcgtcac cggccgccac
     2221 cccatcaatg tcatcgcccg cggtctcggt catacaatgc cgccagcggc agcggtgccc
     2281 tttcagaagt ttcgtcctga gtttttggag gccatcgaca gaggcttgtc actggatccg
     2341 gatcggcgcc cccagaccat caaggcgttc gggcagatgc tgctcgagat cccggaaaag
     2401 atagctccta aaaatagaca gccagctctt caaatggcgg ctagccttgc ggaaggcccg
     2461 ccaccttctg ccttaccccc gaatggcccg ccttcccctg tgaccattcc gcttcccaag
     2521 agtgcggtgc ccgacccgga tggcgagaat gacgatgacg acgatgatga cctcgacgaa
     2581 catcgggact tcaggagcgg gcggcgcggc ttgggaattc tcgttctcgc ggtgctgctg
     2641 gcgcttctga ttccgaccgg tttgtggctg cttgaatcgc agtccggaaa agtgccggaa
     2701 caatcggctc accttgaacc cgaaaaaggc gccgcgagcc aaagcgtgag ttcgccgacc
     2761 gaacctgcac cgccacctgc ggaacggcct cgggggcttg caatggcaaa ggcgcaggca
     2821 aagcaatctg acgccactat ggcagcgcca gcgcagcca
//
LOCUS       DATMGX010001023         2472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_89141, whole genome shotgun sequence.
ACCESSION   DATMGX010001023 DATMGX010000000
VERSION     DATMGX010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89141"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1562)
                     /locus_tag="VNR65_16810"
     CDS             complement(162..1562)
                     /locus_tag="VNR65_16810"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ20379.1"
                     /translation="MAQRFQSTHNGKAPVHSDSNAKRKPRLLDEDHIEALREFADDNE
                     EGRGYYPDSKVFGLTIFLGAKSTVWRFRQQGRSKGKRSSVFKTLGNWPAMDEDEARKQ
                     ALIYSGAVAAGTAAPGKRQAMPFRAAFENYLAHLKAQAEAKGKPPRWWANACKLSEAH
                     IMPQWDGWTLAEMSQNPRAVKTWHAKLSKTIPTTADHCARLIRACYREEARLDRTLNP
                     AASPTSGIRLGKIKVSKAVLDFPDFSAWRKAWDKIENPVHKGYHLAALLTGCRPTELA
                     MIRESDIDLDARRMIIRNAKAGNDISLPITGEIAFALAMAINAPPQTITMKGLRGMKS
                     GEVRVIERKKLHHEIAAPDLVFPGVRQAGHRSGLPVSGNALRHTFRSIAVSLEISEML
                     ISFLMGHSLQGVSARYTNELMIANSQALREAQEKISRRIFELLGLMHGGHHDAPLVPD
                     APTAKKRKATAPARIA"
     gene            1901..>2472
                     /locus_tag="VNR65_16815"
     CDS             1901..>2472
                     /locus_tag="VNR65_16815"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWJ20380.1"
                     /translation="MAARGYSIFDTAIGRCGIAWSHAGVVGVQLPEAREIETRKRLFR
                     LYPDAREQRPPPDVEIAIEAITALLRSEPSDLADVALDLDGIPAFHARVYAFTRRIPR
                     GETITYGEIAAKLRLSGAVHSVAQALARNPFVIIVPCHRVLEAGGYADRISPYGGVIS
                     KRRLLSIEGARGTTTSKTLFDVLLPVAPPRP"
BASE COUNT          406 a          774 c          779 g          513 t
ORIGIN      
        1 gctccgggag gcccgtaggg ccaccggctt ctggaaagcc gtcgccgatc tcaacccggc
       61 ggcggctttt tcatgccccg ctggcggcct tgcggcgcgg ccctactggc ttcccatgcc
      121 gggtccgccc gagagacccc taaagccctc gcctgcccgt ctcaggcgat gcgggccgga
      181 gcggtggctt tgcgcttttt cgcggtcggt gcgtctggca cgagcggggc atcatgatga
      241 ccgccgtgca tgagcccgag caattcgaaa atccggcggc tgatcttctc ttgcgcctct
      301 cgaagagctt gcgagttcgc gatcatcaac tcgtttgtgt acctcgcgga gacgccttgc
      361 agactgtgcc ccatgaggaa cgaaatcagc atctcggaaa tctctagcga gaccgcgatg
      421 ctgcggaacg tatgccggag cgcgttgccc gagaccggaa ggcccgagcg atggccagct
      481 tgacgaacgc ccggaaagac aagatcggga gccgcgatct cgtgatgcag tttctttcgt
      541 tcgatcacgc ggacctcgcc ggacttcatt ccgcgaaggc ccttcatcgt gatggtttgc
      601 ggaggcgcgt tgatggccat cgccagcgcg aaagcaatct cgcccgtgat cggtagcgag
      661 atatcgtttc cagctttcgc gtttcggatg atcatgcgcc gcgcgtcgag atcgatatcg
      721 ctctcgcgga tcatggcaag ctcagttggc cgacagccgg tcaacagcgc cgcgagatga
      781 tagcccttat gaaccgggtt ttcgatcttg tcccatgcct tgcgccacgc cgagaagtcg
      841 gggaagtcga gcacggcttt cgagaccttg atctttccaa gccttatgcc ggaggtcggc
      901 gaggccgccg ggttgagcgt gcgatcaagc cgcgcctctt cgcggtagca ggcccggatg
      961 agccgtgcgc aatggtcggc ggttgtcggg atggtcttcg agagcttcgc atgccacgtc
     1021 ttcaccgctc gcgggttctg cgacatttcg gcgagcgtcc atccgtccca ttgaggcatg
     1081 atgtgcgctt cggaaagctt gcaggcgttg gcccaccatc gcggcggttt gcctttggct
     1141 tcggcttgcg ccttaagatg ggcgaggtag ttttcgaaag ccgcgcggaa cggcatcgct
     1201 tggcgcttgc cgggggcagc ggtgccagcg gcgaccgcgc ccgaatagat cagggcttgc
     1261 ttgcgcgcct catcctcgtc catcgcaggc cagttgccga gcgtcttgaa gacagacgac
     1321 cgcttgccct tagatcggcc ctgttgacgg aagcgccaga cggtagactt cgcgccgaga
     1381 aagatggtca ggccgaagac cttgctgtcg ggatagtagc cgcgtccctc ttcgttatcg
     1441 tcggcgaact cgcgcaacgc ttcgatgtga tcttcgtcga gcaagcgcgg tttgcgtttc
     1501 gcgttcgaat cagaatgaac cggagctttg ccgttgtgcg tgctttgaaa tcgttgtgcc
     1561 atcgtgatgt cactatgatg ccatttgact agttcggatg ctgggatttt cagccgccga
     1621 ggcatcacag ataacactag aagcccgcga aacatgggct ttagacaatg gtttcgggcg
     1681 tttctcgtaa cgtggtaaaa attcggctga ttagattggg aagctgccgt tctgccattg
     1741 aactacgccc gcgagccccc tttgattagc cgagaccgcg cattccgcca agcaccttgg
     1801 cgccggactt tcgtcctctc gcctcgactt taacctgact tgaattttcc agatgcgccg
     1861 tttcgtgacg gtgctatggt gcccgccggg gggtagtagc gtggcggcgc ggggttacag
     1921 cattttcgac acggccatcg gccggtgcgg catcgcctgg agccatgccg gcgtcgtcgg
     1981 cgtgcagctg cccgaggccc gcgagatcga aacccgcaag cggctgttcc ggctctatcc
     2041 cgacgcccgc gagcagcggc cgccgccgga tgtcgagatc gcgatcgagg ccatcacggc
     2101 gctgctgcgc agcgagccca gcgacctcgc cgatgtcgcg ctcgacctcg acggcatccc
     2161 cgccttccat gcgcgggtgt acgcgtttac ccgccgcatt ccgcgcggcg aaaccatcac
     2221 ctatggcgag atcgccgcga agctcaggct ttccggcgcg gtgcattcgg tcgcgcaggc
     2281 gctggcgcgt aacccctttg tgatcattgt gccctgccac cgcgtgctgg aagccggcgg
     2341 ctatgccgac aggatttccc cgtacggcgg ggtgatttcg aagcgccggc tgctgtcgat
     2401 cgagggcgcg cgcggcacga ccaccagcaa gaccctgttc gacgtgctgc tgcccgttgc
     2461 cccgccccgc cc
//
LOCUS       DATMGX010001024         4177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_173284, whole genome shotgun sequence.
ACCESSION   DATMGX010001024 DATMGX010000000
VERSION     DATMGX010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173284"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VNR65_16820"
     CDS             complement(<1..217)
                     /locus_tag="VNR65_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20381.1"
                     /translation="MFERQERDVRSDGAVCLVFGRYAILGSVLLFLFLASQAAPGQAT
                     QPDLTQVNLEDLMNIEVTSVSKKEQRLS"
     gene            complement(488..751)
                     /locus_tag="VNR65_16825"
     CDS             complement(488..751)
                     /locus_tag="VNR65_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20382.1"
                     /translation="MNDLQPLNQNWLFGFVRQNASRVNAEVGTTRSASGRSASPNIAG
                     NGHTPTGKTSASANRANGDDLDACILCMERFVGTALYRNILRE"
     gene            complement(1039..2313)
                     /locus_tag="VNR65_16830"
     CDS             complement(1039..2313)
                     /locus_tag="VNR65_16830"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HWJ20383.1"
                     /translation="MFSASLHKIPAYILLTMFLMPCVSAQEMSAIPARLVIPDGTAIK
                     LRLAESVSSARAHVGDLLDFIVVRDVSVGGFTVIPAGTMARGSVTGVKGRRILGIGGK
                     VSLKLDSVELVNGDRVGLRGSKEVKGGSRTKLMAAAMIATALIFLPATPVFLLTRGHT
                     STVVKSTEITAQIDGATSVLSAGLLHSRESSSQLDEMMGYLPPRVFNGEGREGDMVNL
                     VFVAQAEDLQEAFKRAGWVKTDKWNPVFVWHLLRHRTNDTRVPMARFYLFGRVQDYSY
                     ALPDPDAIVSRRHHLRIWKTDYTIDGTPIWTGAATHDVAIEIAKRGHLINHRIDPAVD
                     AERDFIGANLTDTSSVSRQEYLHCVDPVFQAQTASGETYHSDSRILLLDLHPGTPART
                     GVPREPLTVVRATALPIAVPAESFQSDLMSPR"
     gene            complement(2577..3323)
                     /locus_tag="VNR65_16835"
     CDS             complement(2577..3323)
                     /locus_tag="VNR65_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWJ20384.1"
                     /translation="MKAMILAAGLGTRLRPLTDDRPKALVEVAGRTMLEIALARLRAF
                     GIGEVIVNVHHFAGMVLDYLKTNDNFGMRIEVSREEVLLDTGGGLKKAAHFFLEDSSD
                     CDEPFLLHNVDVISTIDLRRMVQLHIENRALATLAVQARESSRYLLFDQQFQLCGRRS
                     GRDRETELVRPSEQMQALAFCGIHIISSHLFAKMTEADAFSIVDCYLRLAGREERILA
                     FRADECYWRDLGTPDKVMQAAQDLKQKVLL"
     gene            complement(3320..>4177)
                     /locus_tag="VNR65_16840"
     CDS             complement(3320..>4177)
                     /locus_tag="VNR65_16840"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWJ20385.1"
                     /translation="YFLYRDFQSRNVMLSAGNPFFLDYQGGRRGALQYDIASLLFDAK
                     ADLPPELRQQLLDHYLDTLAGFIEIDRELFMQHFYAYVYVRIMQALGAYGFRGFYERK
                     PHFLQSVPYALKNVRWLLHNVNLPIALPILMDAFKSILGSDKLQGLAPGPGNVTVRIC
                     SFSFHRGPLKDEAGHGGGFVFDARSLPNPGREERFRTLTGKDEPVADYLNQQESVHQF
                     LASAAALVDASVSDYQRRGFKSLMASFGCTGGQHRSVYLAEQLAKHLRGRNGVEVLVR
                     HRELENPGE"
BASE COUNT          943 a         1205 c         1137 g          892 t
ORIGIN      
        1 gtgacaacct ttgttctttc ttggagacgg aagtcacctc gatgttcatc aggtcttcga
       61 ggttcacctg cgtaagatcg ggttgcgtgg cctgcccggg cgctgcctgg gatgccagga
      121 acaggaagag gagaactgac cccaggatcg cgtaccttcc gaaaactaga cacaccgcac
      181 catcgcttct gacatctctc tcttgtcgct caaacacgac aacccttcct ccctcaactg
      241 cccctatcgg cacagtttcg ctggaagtac agtcgagagc acttcggacg aagccaggtc
      301 cccccgcagg accggctgcg ccggatttgc cgagagctgt ccctcgatcc cgcgattgga
      361 atcgcaacag gatttctttc gtaagttggg gtcaaggttg tgacgatgaa tcgcagactt
      421 caggagaatg gcatacacga atccgctgag aagctcttcg cccacgtgtc gggccagagc
      481 gacgccctta ctcgcgcaag atgttgcggt acaatgctgt gccgacaaac ctttccatgc
      541 acaggatgca cgcatcgagg tcgtcgccgt tggctctgtt agcgctggcg ctggtttttc
      601 cggtgggggt atggccgttt ccggcgatat tggggctggc gctcctgcca cttgcggagc
      661 gggttgtgcc aacctcggca ttcactcgtg acgcattttg ccgcacaaaa ccgaacagcc
      721 agttttgatt aagtggctgc aaatcattca tttagcttat cttctgattt gggtgaatcg
      781 gcctcaaaat ccgccgggct tcggcccttg gggattcgat tccccctccc ggcaccaaca
      841 aaatagatcg cttacggcaa gacccgaatg tcttgctacc tggcaaaatc cctgagcttc
      901 agtgttctga agaaggatac ctgcgtccgc cgtttcccag cattccacct tgctggcaca
      961 gccaccagga cggaccccat caaactcgca gccgacggca atcggcacat ccgccgattt
     1021 acctctacct cgagtcagtc aacgcggcga catcagatcg ctctggaaag actccgctgg
     1081 aactgcgata ggcagagcgg tcgcccgaac aacggtcaat ggttccctgg gcacgccggt
     1141 cctagccggg gtgcccggat ggagatccag gagaagaatt ctgctgtccg agtgataggt
     1201 ctcgcctgac gcagtttgcg cctggaaaac cggatcaaca caatgcagat attcttgccg
     1261 actgactgac gaggtgtctg tcaggttggc tccgatgaaa tcgcgctctg catctaccgc
     1321 agggtcgatc ctgtggttga tcaaatgccc tcgcttcgcg atttcaatcg ccacgtcgtg
     1381 cgtggcggcg ccggtccaga tgggcgtccc atcaatcgtg tagtcggttt tccagattcg
     1441 aagatgatga cgccgggaga cgatcgcatc cggatcgggc aaggcgtacg agtagtcctg
     1501 cactcttccg aacaggtaaa acctggccat cggcaccctc gtgtcgttgg tccgatgtcg
     1561 caatagatgc catacgaaaa ccggattcca tttatcggtt ttgacccaac ccgcgcgctt
     1621 aaaggcttcc tgcaagtctt ctgcttgggc gacaaacacc aggttcacca tgtcgccctc
     1681 gcgtccctca ccattgaaga cacgtggcgg cagataaccc atcatctcgt ccagttggga
     1741 agaactttct cgcgaatgca gcaaccctgc cgacaggacc gaggtggcgc cgtcgatttg
     1801 cgcagtgatt tccgtacttt tcacgaccgt gctggtgtgg ccacgggtga gcaagaaaac
     1861 cggcgtggcg ggcagaaaga tcagggcagt ggcgatcatg gctgcggcca tcagcttggt
     1921 acgggagcct ccttttactt ccttgcttcc acgcaagccg acccgatcgc cgttgaccag
     1981 ctctacagaa tcgagtttca gggatacttt gccgccaatt cccaggattc gcctgccctt
     2041 aactccagtg acggagccgc gagccatggt tccggctgga atgacggtga agcctccaac
     2101 gctcacatct ctcaccacga taaaatccag gagatctcca acgtgggcgc gggcagagga
     2161 aacactctcc gcaagtcgaa gcttaatggc agtaccgtct ggaatcacca gcctggcagg
     2221 aatcgcactc atctcctggg ccgaaacaca aggcatgaga aacatcgtga gcaggatgta
     2281 cgccggtatt ttgtggagag atgcgctgaa caatttatgc cgcctttact tgaggcctag
     2341 cctctcaccc gcgacggcca agaatgtcta gcgcaatagc gtggtggata cgaaagtcta
     2401 ccgtactcgg gatatgtaaa tgtgtgcaca attgcacatt acaaagatgt cattgtggct
     2461 ggacccgagg aatgcggccc gtgtgtgtgt agaaaacctt tgagcagtcc cttggcgatt
     2521 gattccgcac ccagtcccag tcccggtctc gatctgtcat cggtgcggcg aacttattac
     2581 agaagaactt tttgttttag atcctgagcg gcttgcatca ccttgtccgg cgtgccgaga
     2641 tcgcgccaat aacattcatc ggcccgaaaa gcgagaatcc tttcttcgcg gccagcgaga
     2701 cgcaaatagc agtcgacgat ggagaacgcg tctgcttccg tcatttttgc aaaaaggtga
     2761 gaagaaatta tgtggatgcc gcaaaaagcc agcgcctgca tctgctctga aggtcgaaca
     2821 agttcagttt cgcgatcgcg cccggatcgc cggccgcaaa gttggaactg ctggtcgaac
     2881 agcaggtagc gagaactctc ccgggcttgc accgcaagtg tggcaagagc ccgattttca
     2941 atatgaagtt gcaccatgcg ccggagatcg atcgtgctga tgacatcgac attgtgcaaa
     3001 aggaacggct cgtcacagtc gctggaatcc tcaagaaaga aatgggcggc tttttttagg
     3061 ccgccaccgg tgtcgagcaa gacctcctcg cgagagactt caatacgcat gccgaagttg
     3121 tcgttcgttt tcagatagtc gaggaccatg ccggcaaagt gatgcacatt gacaattact
     3181 tcgccaatcc caaaggcgcg taagcgagcg agcgcgatct ccaacatcgt gcgcccagcg
     3241 acttccacca gcgccttggg acgatcgtcg gtgagcggcc gcaagcgcgt gccgagcccc
     3301 gcggctaaaa tcatcgcttt cattcgcccg gattctccaa ttcacgatgc cgcaccaaca
     3361 cttccacccc gttcctgccg cgcaaatgtt ttgccaattg ctccgccaga tataccgagc
     3421 gatgctggcc gcctgtgcaa ccaaacgacg ccatcaagct cttaaagccg cgacgctgat
     3481 agtcgctcac gctggcatcc accagcgccg ccgcgctagc gaggaattga tgcacactct
     3541 cttgctgatt gagatagtcg gccacaggct cgtctttgcc cgtaagagtt ctgaagcgct
     3601 cttcgcgtcc ggggttggga agactccggg catcgaaaac gaaaccgccg ccgtgcccgg
     3661 cctcgtcttt cagcggaccg cggtggaatg aaaaactgca aatccgcacc gtgacatttc
     3721 ctggtccggg agcgagaccc tgcaatttgt ccgagcctag aatgctcttg aacgcgtcca
     3781 tcaggatcgg cagcgcgata ggcagattga cgttatgaag cagccagcgt acgtttttca
     3841 gcgcgtacgg cacgctttgc agaaaatgag gtttgcgctc ataaaagccg cgaaagccat
     3901 aagcgcccaa cgcctgcatg atgcgcacgt aaacgtaggc gtagaagtgc tgcatgaaca
     3961 gttcgcgatc aatctcaata aaacccgcaa gcgtatcgag ataatgatcg agcaattgct
     4021 ggcgcagctc gggcggcagg tcggccttgg catcgaacaa tagagaagcg atgtcgtatt
     4081 gcagcgcgcc tctgcggccg ccctggtaat ccagaaaaaa tggattgccg gcgctcaaca
     4141 tgacattgcg cgattggaag tcgcgataga gaaagta
//
LOCUS       DATMGX010001025         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_89248, whole genome shotgun sequence.
ACCESSION   DATMGX010001025 DATMGX010000000
VERSION     DATMGX010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89248"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..706
                     /locus_tag="VNR65_16845"
     CDS             56..706
                     /locus_tag="VNR65_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20386.1"
                     /translation="MSTKFVGAIAVVTAVATTVAFAQGQPAPKASKADVQKLVGSIKS
                     DKGKLAKYCEITKLEVQAGALAQKNQNDPKLETIGKQMDDIAGKLGPDYEKIINSDLD
                     DASAALFDDLSKSCNVGFAAPQAAPKVSKADVQKLIGSIKGDKIKLGQFCEVTKLASQ
                     AGSLAQKNRNDPKLQTLGKQMADLTAKLGPDYERITTSDLDEASTAPLDDLYKNCK"
     gene            complement(725..>2290)
                     /locus_tag="VNR65_16850"
     CDS             complement(725..>2290)
                     /locus_tag="VNR65_16850"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWJ20387.1"
                     /translation="LSRSLVESTSVPEPSEGRRVTANPRLQVASFAQAPATIVATSCR
                     DPSMLYNLLQRTRRNRDRHLVAAFVDQSTGLAHDIVDVACNVRELSHEIATQQLHVCE
                     VQDGAALLQEETARIVETAAEGNAVAREANDEIQGSAIIVRESLSAISELGQTVTEDS
                     HLLSSFADTLDRVSSFAQSIETIAKQTHLLALNATIEAARAGSAGRGFAIVAQEINAL
                     AAKTAHVTKSISDTVVDLNKKAQQLSVQGAKSADAANRAEKSTSAILETLNTIEQRVS
                     QFVDEMSRIDGSAKSVQTKSADMSEAVASLSSGFLSATAHFERMEKSTDHLQAVSEGL
                     LTTTITTELETPHSRFVQEAMRLASKAGTILSGVIDAGDISIAQTFDKKYRPIPGSDP
                     AQFETSYCKVFDRLLQSLFDGALNFDPRIVFCTAVDVNGFLPTHNSKFSQPQGQDAVW
                     NAAHCRNRRFFKDRVGLAAGKNEKAFLLQTYSRDMGGGRFVPMVDVSAPIYVQGRHWG
                     GLRLAYALDFTSK"
BASE COUNT          526 a          623 c          643 g          498 t
ORIGIN      
        1 gagagcccta cccgcgcgcc gctctgagaa ccgaaacaaa attagggagc tatttatgag
       61 cacgaagttc gttggcgcta tcgcggtcgt taccgccgta gcaaccacgg ttgccttcgc
      121 acagggccaa cccgcgccga aggcgagcaa ggctgacgtg cagaagcttg ttggaagtat
      181 caaaagcgat aagggaaagc tcgctaaata ttgtgagatt acaaaactgg aagttcaagc
      241 cggcgcactg gctcagaaga atcagaacga tccaaagcta gagactattg gaaagcaaat
      301 ggatgacatt gccggaaagc ttggtcctga ttacgaaaaa atcataaact cagacctcga
      361 cgacgcttcc gctgcacttt tcgatgatct ctccaaaagc tgcaatgttg gattcgcagc
      421 gccccaagcc gcgccaaagg tgagcaaggc cgatgtgcag aagcttattg gcagtattaa
      481 aggcgataaa ataaagctcg gtcaattctg tgaggtaacg aaactggcga gtcaagccgg
      541 ctcgctcgct caaaaaaacc gaaacgatcc aaaattgcaa acgcttggga agcaaatggc
      601 cgaccttact gcgaagcttg gtcctgatta cgaaagaatc acaacttcag acctggacga
      661 agcttcaact gcgccgctcg atgacctcta caaaaactgc aaataggtct gcgcaacgac
      721 acactcattt tgaggtgaaa tcgagcgcat aggcgagccg cagcccgccc cagtgccggc
      781 cctggacata gatcggggcc gatacatcga ccatggggac aaaccggccc cctcccatgt
      841 cgcggctata agtctgcagg agaaacgctt tttcattttt cccggccgca agtccgaccc
      901 ggtctttgaa aaagcgccga tttctgcaat gtgcggcgtt ccaaaccgca tcctgtccct
      961 ggggttgaga gaacttgctg ttatgcgtcg gaagaaagcc gtttacgtca acggccgtgc
     1021 agaagacaat gcgggggtcg aagttcagcg ctccatcgaa aagcgactgc agcaggcgat
     1081 cgaacacttt gcagtaactc gtttcgaact gcgccggatc cgagcctggg attgggcgat
     1141 atttcttgtc gaatgtttgc gcgattgaaa tatcgcctgc gtcgatgaca cccgaaagta
     1201 tcgtgccagc tttggacgcc agtcgcatcg cttcctgcac gaaacgcgag tgtggcgtct
     1261 caagctcggt tgtaatggtg gttgttaaca ggccctcgct gacagcctgc aaatgatcgg
     1321 tcgatttctc catccgctcg aaatgtgctg ttgccgaaag aaacccgctt gaaagagaag
     1381 cgaccgcctc gctcatatca gcgcttttgg tttggacaga tttcgccgat ccatcgatcc
     1441 tggacatctc gtccacgaat tggcttacgc gctgctcaat ggtgttgaga gtttcaagga
     1501 tcgcggacgt gcttttttcg gctctgtttg cggcgtctgc gcttttggcg ccctggaccg
     1561 aaagctgctg cgcctttttg ttcagatcga caaccgtgtc cgatatgctc ttcgtcacat
     1621 gagcggtttt ggccgcgagc gcattgattt cctgtgcgac gatcgcgaag ccgcgcccgg
     1681 cggaccccgc gcgggcggcc tcaatcgtcg cgttgagcgc aagcagatgc gtctgctttg
     1741 cgatggtctc gatgctctga gcgaaggagc tgacgcgatc taaagtgtcg gcaaacgatg
     1801 aaagaaggtg gctgtcctcg gttacggtct ggcccagttc ggagatcgcc gaaagcgact
     1861 cccggacgat aatcgccgat ccctgaattt catcgttggc ttcccgggcg acggcattgc
     1921 cttccgctgc ggtttccaca atgcgggccg tctcttcttg cagcagcgcg gctccgtcct
     1981 gaacctcgca cacatggagt tgttgcgtcg ctatctcgtg agaaagctct cttacattgc
     2041 aggcgacatc gacaatgtcg tgagccaagc cggtcgactg atcgacgaaa gctgcaacca
     2101 gatggcggtc tctgttccgc cgtgtgcgtt ggaggagatt ataaagcatc gatgggtccc
     2161 ggcacgaggt cgccactata gtggcgggcg cctgtgcgaa actggctact tgtagccgag
     2221 ggtttgctgt gacccggcgg ccctcgctgg gctccggcac cgacgttgac tctacgagcg
     2281 atcgggaaag
//
LOCUS       DATMGX010001026         2702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_58275, whole genome shotgun sequence.
ACCESSION   DATMGX010001026 DATMGX010000000
VERSION     DATMGX010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58275"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1365
                     /locus_tag="VNR65_16855"
     CDS             61..1365
                     /locus_tag="VNR65_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20388.1"
                     /translation="MFLMFLCAIGLWVWGGNALNKRISRDIGKDSLFWKTETATLLSN
                     AGFFAFIYLLCGMWNLGLVILFVVAGQLSIIMALVLNSLLGSQGEKFTSRVFAGGGEL
                     GMKYPKFSRFALAMLVVLLIAYLIAAGIIHFRYPWESPVLVAASVKYTLLFFIGSYPF
                     LFTEVIATITSENLDEDTRQGIFISQMTAVIPTALFAALALWAFGAGGVDLPASLGNL
                     SHSFSLRITLILIAYFGGLILLPYFIGTQRGKRKRLALLHERQEYVRTLADILESPAG
                     SRYVPKLTELHDQVAAERETVKQNHQLMLLDDWKTNKPADFPEQAAPLVEAFRHSREL
                     DPRSKFFDYLTKFENELQEIIDDLKARPPETVEDAAAKWSKTYETRHAQLGEEIKGAT
                     SAAPLVTVAVGTVATAIVSPILAGVGKAAWAWISAAYGLKVQ"
     gene            1715..>2702
                     /locus_tag="VNR65_16860"
     CDS             1715..>2702
                     /locus_tag="VNR65_16860"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWJ20389.1"
                     /translation="MSEAIAKQTASAPAPLVSLTDDETLFRDTVRQFADESVRPHVKE
                     MDEKGVFEPKLIEQFFQLGLMGIEVPEQYGGGGGTFFEAILAVEELSRVDPSAGVIVD
                     VQNTLVNNALLRWATEEQKKRYLPRMCTDTVGAYALSEAGSGSDAFALQTKAELKGDE
                     YVLNGQKLWITNAKEAGVFILLATVDPAAGYKGITAFIVEKSFPGFTVGKKEDKLGIR
                     ASSTCELILDDCRVPKNNLLGDAGKGYKIAIETLNEGRIGIGAQMLGLARGAWEFAVK
                     YAQERKQFGRAIAEFQGIQFQIAQMATEIEAARMLLYNCARMKDAGMNFLKEA"
BASE COUNT          558 a          756 c          820 g          568 t
ORIGIN      
        1 gtcgcatcgc gggacgcaag cggaggcgaa caggatgggc gggttatgca taagctttta
       61 ttgtttttga tgtttctgtg tgctatcggg ctatgggtgt ggggcggaaa tgcgctcaac
      121 aagagaatat cccgggacat cggaaaagat agcctgttct ggaagaccga gactgcaacc
      181 ttgctgagca acgcgggttt ttttgcgttt atctatctgc tctgcggtat gtggaacctg
      241 ggcctggtga ttctgtttgt ggtggcggga cagttgagca taatcatggc gctggtgctt
      301 aactcactcc tgggctccca aggcgagaag ttcacgtcgc gggtatttgc gggaggtggg
      361 gaactgggga tgaagtatcc caagttctcc cgcttcgctt tggcgatgtt ggtcgtgctg
      421 ttgatagcct atctgatagc cgctgggatc atccattttc gctatccttg ggaatcgccg
      481 gtgctggtgg ccgcgagcgt caaatatact ttgctgttct ttatcggcag ttatcctttc
      541 ctctttaccg aagtcatcgc caccatcacg tcagagaacc tggatgaaga tacccgccag
      601 ggaattttca tcagtcaaat gaccgctgtt atcccgacag cgctgtttgc ggccttggcg
      661 ctgtgggcgt ttggggctgg cggagttgac ttgccggcgt ctttggggaa cttgtcccat
      721 tcgttctccc tccgaatcac tctcatcctc attgcctact tcggcggatt gattctcctt
      781 ccatacttca tcggtacgca acgaggaaag cggaaacgac tggccttgct gcacgaacgc
      841 caggaatatg tgcgcacgct cgccgacata ctcgagtcgc ctgccggttc ccgctacgtg
      901 ccaaaactca ccgagttgca cgatcaggtt gcggccgaac gggaaaccgt caagcagaac
      961 caccagctca tgctcctgga cgattggaag acgaacaagc cggcagattt tcctgagcaa
     1021 gctgctcccc tggttgaagc tttccgccac tcccgcgaac tggatccgcg ttccaagttt
     1081 ttcgattact tgaccaagtt cgaaaacgag ctgcaggaga tcatcgacga cctgaaggca
     1141 cggcccccgg aaacggtaga ggatgccgcc gccaagtgga gcaagactta tgagacgcgc
     1201 catgcccaac tgggcgagga gatcaaaggc gctaccagcg cggcgcccct ggtcacggta
     1261 gcggtgggga cggtcgccac ggccatcgtc agcccgattc tggccggcgt gggcaaggcg
     1321 gcctgggcgt ggatctcggc ggcgtacggg ctgaaagttc agtaaaggaa atcgctgcca
     1381 agcgagggcc catacgtgag gctcgtagaa gattgccacg ccgcagatgc aagacagcgg
     1441 gatgtccagc cggttggcga tgtattgcgg agtctcccta ggaaggtact tatgggcgtt
     1501 acgctcctgt accgcctcca gaatgccgag caattcgccg ggacgcccac tccgctgggc
     1561 ggaaagggaa ccgccacatc atgggaatgc aggcgtggta gtcccaagac ctttgtttcc
     1621 cgctcgtgtc gttccacagg aacgagagtt cgtcggcggt gtcgcggatg ctagaatcga
     1681 aggtctccta cccctgtctg gaaggattgc tctcatgtca gaagccattg caaagcaaac
     1741 cgcctccgca cccgcccctc tcgtttcgct taccgacgac gaaaccctct tccgcgacac
     1801 ggtccgccag ttcgcggacg agagcgtccg gccgcacgtc aaggagatgg acgagaaggg
     1861 tgtgttcgag cccaagctca ttgaacaatt ctttcagttg ggtttgatgg gcattgaagt
     1921 tcccgagcaa tacggcggag gaggcggcac cttcttcgag gcgatcctcg cggtggagga
     1981 gctttcgcgc gtcgatcctt ccgccggagt tatcgtggac gtgcagaata cgctggtcaa
     2041 caacgctctc ctgcgctggg ccacggaaga gcagaagaag cgctacctgc ctcgcatgtg
     2101 tactgacacc gtgggcgcct atgcgttgag cgaagccggt tcggggtcgg atgccttcgc
     2161 cctgcaaacc aaggcagagc tgaagggcga cgagtatgtc ctcaacggcc agaagctgtg
     2221 gatcaccaac gccaaggaag cgggcgtctt catcctgctg gccacggtcg atcctgccgc
     2281 cggatataaa ggcatcaccg ccttcatcgt cgagaagagc tttcccggat tcaccgtcgg
     2341 caagaaggaa gacaagcttg gcattcgcgc ttccagcacc tgcgaattga tcctggacga
     2401 ttgccgcgtg cccaagaaca accttctggg cgatgccggt aagggctaca agatcgccat
     2461 cgagacgctg aatgaaggcc gcatcggcat cggcgcgcag atgctcggac tggcacgcgg
     2521 cgcgtgggaa ttcgccgtca agtacgcgca ggagcgcaag cagtttggcc gcgccatcgc
     2581 tgagttccag ggcatccagt tccagatcgc gcagatggcc accgagatcg aggcggcccg
     2641 catgctgctt tacaattgcg cccgcatgaa agacgcggga atgaacttcc tgaaggaagc
     2701 gg
//
LOCUS       DATMGX010001027         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_438351, whole genome shotgun sequence.
ACCESSION   DATMGX010001027 DATMGX010000000
VERSION     DATMGX010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438351"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1340
                     /locus_tag="VNR65_16865"
     CDS             81..1340
                     /locus_tag="VNR65_16865"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWJ20390.1"
                     /translation="MIIVRLALQSLANRWLAALLTIFSIALSVMLLLGVEKVRTGARQ
                     SFADTISGTDLIVGARSGSLNLLLYSVFRIGNATNNITWKTYQDIAARPDIAWIVPIS
                     LGDSHRGFRVMGTTPDYFRLYHYRHGRPLEFASGAPFSDLFDAVIGADVAAELGYKTG
                     DKIIVAHGLGSVAFVEHDDKPFRVSGVLAKTGTPVDRTVHVSLPAIEAIHVDWQSGAR
                     IPGQSVSADEVRNMGLEPKAITAAFIGLKSKLAAFRLQRAINDYPEEPLSAILPGAAL
                     QELWGLVGTAETALSAVSGMVVVTALLGMVTMILTTLNERRREMAILRSVGARPLTIL
                     SLLVTEAGVLTFCGVALGTCLLYVTLIVLQPFIDRTYGLHIAVDPPSWDQLAVLAAIV
                     AAGFIAGLLPALRAYRLSLADGMTVRT"
     gene            1354..1929
                     /locus_tag="VNR65_16870"
     CDS             1354..1929
                     /locus_tag="VNR65_16870"
                     /inference="COORDINATES: protein motif:HMM:NF023164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HWJ20391.1"
                     /translation="MTRILHAAALCLLIVATPSLATEGEPAMDLAWGKLVPAAPQPVQ
                     TMKAKSFLSGATPFAGAGGSEPPPPPTVDDKRWLSERRLQPGSGGTPAVVTDLDGKRV
                     RIGGYVVPLDFEATTVKEFLLVPFVGACIHVPPPPPNQIIYVKTAKGFDVAGSFDPVY
                     VTGTLKVASQYTGLAETGYTIDADKVDTRKE"
     gene            2603..>2805
                     /locus_tag="VNR65_16875"
     CDS             2603..>2805
                     /locus_tag="VNR65_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20392.1"
                     /translation="MAAISSNGPSKLHCKPLVASIAVLSFACTVPGTLHAGVPQEAPK
                     AAQQDPRATAQEKRPGETLSERL"
BASE COUNT          539 a          883 c          812 g          571 t
ORIGIN      
        1 ggagagggag caggccgcgc cccttgcaac ggcgtacctt ctccccttgc gggagaagga
       61 caggatgagg ggggccaacc atgatcatcg tccgcctcgc gctgcaatct ctcgccaatc
      121 ggtggctggc tgcgctcctg accatcttct ccatcgcgtt gagcgtgatg cttctcctcg
      181 gcgtcgaaaa agtgcgaacc ggtgcccggc aaagcttcgc cgacaccatc tcgggcaccg
      241 atcttattgt cggcgcgcgc agcggaagcc tcaatctgct cctctattcc gtcttccgca
      301 tcggcaacgc gacgaacaac atcacctgga agacctatca ggacatcgcg gcgcgcccgg
      361 acatcgcctg gatcgtgccg atctcgctcg gcgacagcca tcgtggcttc cgcgtgatgg
      421 ggacgacgcc ggactatttc aggctctacc attaccggca tggtcggccc ctggaattcg
      481 catccggcgc gcctttcagc gatctcttcg atgccgtgat cggtgccgat gtggccgccg
      541 aactgggcta taaaaccggc gataaaatca ttgtggccca cggattaggc tccgtcgcgt
      601 tcgttgagca cgacgacaag cccttccgtg tttccggcgt gctcgccaaa acaggtacac
      661 ccgtcgaccg caccgtccat gtcagcctcc ccgccatcga agcgatccac gtagattggc
      721 agagcggcgc gcgcattccc ggccagagcg tctccgctga cgaggtgcgc aatatgggcc
      781 ttgagccgaa ggcgatcacg gcggcgttca tcgggctgaa gtcgaagctc gcggcgttcc
      841 ggctgcagcg cgccatcaac gactaccctg aggagcctct gtccgccatc ctgccgggag
      901 ctgcgcttca ggaactctgg gggcttgtcg gaacggcaga aaccgctctc tcggctgtct
      961 ccggcatggt cgtggtgacc gcgcttctcg gcatggtgac gatgatcctc acgacgctga
     1021 acgagcgacg ccgcgagatg gcgatcctgc gctcggtcgg cgcacggcct ttgaccattc
     1081 tctcgcttct tgtcacggag gctggcgtcc ttacgttttg cggggtcgcg ttgggcacat
     1141 gtcttcttta tgtcaccttg atcgtgctac agccattcat agatcgaact tacggcttgc
     1201 atatcgccgt cgatcctccc tcgtgggatc aactggctgt tctggcagcc attgtggcgg
     1261 ctggctttat cgctggcctg cttcccgctt taagggctta ccgcctgtct ttagcggacg
     1321 gcatgaccgt ccgaacctag acggaaagaa cagatgactc gcattctgca tgctgccgct
     1381 ctttgccttt tgatcgtggc gacgccgagc cttgccacgg aaggcgagcc cgcgatggac
     1441 ttggcttggg gcaagcttgt cccggcagcg ccgcagccgg tccaaaccat gaaggcgaaa
     1501 agcttcctgt ccggcgcgac gccatttgcc ggcgcaggcg gaagcgagcc ccctcctccg
     1561 cccaccgttg atgataagcg ctggctgtcc gaacgccggc tccagcccgg atcgggcggg
     1621 acacccgccg tcgtgacgga cctggacggc aaacgggtgc ggatcggcgg ctatgtggtg
     1681 ccgctcgact tcgaggcaac gaccgtcaag gagttcctgc tcgttccctt cgttggcgcg
     1741 tgcatccacg tcccgccgcc cccgccgaac cagatcatct atgtgaagac ggcgaaaggc
     1801 tttgacgttg ccggatcatt cgacccggtc tacgtcaccg gaacgctgaa agttgcgtcc
     1861 caatatacag gcctcgccga gaccggatat acgatcgacg ccgacaaggt cgatacgcgc
     1921 aaggaatagc cgtacgacgc caatcccgca gaaggatttc attccgcccg ccgctcgctc
     1981 cgtatgtcgt catccccgcc gagcaaaacg cgagcgagga tccacaaggt gcaggcgcct
     2041 ccttgtttcg actcccggat aaggcttcgc ccttccggac cttgatgacc ccgctatctc
     2101 cgcctcgtga gcttacagtt cgactttttc gaactgtaag gcagcagccc ccaaggcgtc
     2161 cgccatggtc atggtgaatt gctcgttgcg gcgccgaaaa ggcgttaacg aaaggatggt
     2221 taatttcggc gcgccggatt tatttctggt ccaattacgc ttaattaggc ttaaccatac
     2281 attgatccgg cgcagaacga ggctacgagc ggcgctagac aggcggttct aatgcgttga
     2341 aagtgatcag ctttttttca ctgatcggcc ccattaaata gaatttaatg ccggaatttt
     2401 cccattaact gggcgcgtcg gcggaaacaa cggggtaatc aagtaaaaaa ttggatttgt
     2461 gcctgaccac tcaagaacgc gcaccctcat ggcgcgggtt agccatgcgc ggctcatctc
     2521 gcctgccaga gccgcgaact gttggccgtg aggaaccaga gaggtttcga ccggtttccg
     2581 aatggaaaac tgaggaagaa ttatggctgc aatctcgtcc aacggacctt caaagcttca
     2641 ctgcaaaccg ctggtcgcat cgatcgcagt cttgtcgttc gcttgcacag tgccgggcac
     2701 acttcacgca ggcgtaccgc aggaggcgcc caaagccgca cagcaagatc cacgcgcgac
     2761 ggcgcaggag aagcgcccgg gtgaaactct gagcgaacgc ctcga
//
LOCUS       DATMGX010001028         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_371939, whole genome shotgun sequence.
ACCESSION   DATMGX010001028 DATMGX010000000
VERSION     DATMGX010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371939"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..913)
                     /locus_tag="VNR65_16880"
     CDS             complement(260..913)
                     /locus_tag="VNR65_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ20393.1"
                     /translation="MVDNLRPECTRSVRAPGRPRSFDPDDALDAAVRHFWACGFDRSD
                     MAGIANSIGVTKPCLYQLFGDKEQLFLRALEHYGATIGAAPVAAFLAAETIQGGVSAF
                     FEVAIKGQTEPGRPSGCLMACVALARAEDSAKVKALYAKALSMTAATLKERFDEKIAT
                     AMLPPGYPSAKSAALMVDLMQALALRARAGASRDELLALGHDYTALVCNLGFQPIHD"
     gene            992..1747
                     /locus_tag="VNR65_16885"
     CDS             992..1747
                     /locus_tag="VNR65_16885"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ20394.1"
                     /translation="MGVLTGKRALITGGTTGIGLATAKAFLAEGARVAITGQSRDRLD
                     QAQSELGIDVLCIRADAGRVADAETLITEVGQAFDGLDVLFLNAGIAKFAPLADVTEA
                     FFDEQFAINVKGVLFTAQKAAPLMAAGGSIVVNTSVNNRMGMAGTLVYAASKAAARSL
                     VRVLAAEFASRGVRVNAISPGPIETPIFGKLGLPDEQLQSIAGGLIQKIPLGRFGHAD
                     EIAKAVLFLASDNASFITGVELVADGGWTEIMP"
     gene            1744..>2079
                     /locus_tag="VNR65_16890"
     CDS             1744..>2079
                     /locus_tag="VNR65_16890"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWJ20395.1"
                     /translation="MTAGATPIGVLIAGVGPGLGLALARCFARAGHNVAMLARQGDRL
                     GEFASLDRDHLVPIACDLTDPTAVTAAFETAEKKNGPLECAVFNAGTFRPGSILDTTP
                     EDFEHCWRIG"
BASE COUNT          402 a          634 c          651 g          392 t
ORIGIN      
        1 tgtcgtcctt cctcaaggac gacaggtggc ctcaaaatcc ccgcggcgcg caatgtgatg
       61 ctgacacgtc gcttacgtcc atctcaaata ttcctgccct acagattcgt cggaaacaaa
      121 cagattcaag aggttaaatg cgcatcccgt aaccaactca aaagactcaa ttcccttccg
      181 gacggacact aagttccggc acgaagcacg ttccgtgcgg gctttaactg ggaaatgtca
      241 ggttgaagcc aatcggagat tagtcatgaa taggctgaaa accaaggttg caaaccaaag
      301 ccgtgtaatc gtggccgagg gcgagaagtt cgtctctcga agcgccggcg cgggcacgta
      361 gtgccagtgc ttgcatgagg tcgaccatga gcgcggcact tttcgcgctt ggatagccgg
      421 gtggcagcat ggcagtggca attttctcgt cgaagcgctc cttcaaggtc gcagcggtca
      481 tgctcagcgc cttggcgtaa agcgccttca ccttggcact gtcttcggcg cgggcgaggg
      541 cgacgcacgc catgagacac ccgctcgggc gtccaggctc ggtctgtccc ttgatcgcga
      601 cttcgaaaaa cgcgctcaca ccgccctgga ttgtttcggc tgcgaggaag gcagccaccg
      661 gagctgcccc gatggtcgcg ccgtaatgtt cgagcgcgcg caggaacagc tgctccttgt
      721 cgccgaagag ttgataaaga cagggttttg tcaccccgat ggaattggca atgcctgcca
      781 tgtcgctgcg gtcgaaaccg caggcccaaa aatggcgcac cgcggcgtcg agcgcgtcat
      841 cggggtcgaa gctgcgcggc cttcccgggg cgcgaaccga gcgggtgcac tccggtcgaa
      901 gattgtctac caacgggtaa caaactcctt gacgcctctt ggcacacccc atagcctact
      961 cctcggtagg gaatgaaaca aggagcgtcg catgggcgtg ttgacgggaa aacgtgcgct
     1021 gatcacgggc ggcacgaccg gaatcggtct cgcgacagcc aaagcgtttc ttgcggaagg
     1081 cgcccgcgtt gcgatcaccg gccagagccg agaccggctc gatcaggcgc aaagcgagct
     1141 tggtatcgat gtcctctgca tccgcgcgga cgccggacgc gtcgccgacg ccgagacgct
     1201 gataactgag gttgggcagg ccttcgatgg gctcgacgtt ctctttctca atgcgggtat
     1261 cgcgaagttt gcgccgctgg ccgatgtgac cgaggctttc ttcgacgagc agtttgcgat
     1321 caacgtaaag ggcgtgctgt ttacggccca gaaggcggca ccgctgatgg cagccggcgg
     1381 cagcatcgtc gtcaacacgt cggtcaacaa ccgtatgggt atggcgggaa cgctcgtcta
     1441 cgctgccagc aaggctgcgg cacgctcgct cgtgcgcgtg ctggccgccg agttcgcatc
     1501 gcgtggcgtg cgggtcaatg ccatcagccc ggggccgatt gagacgccga tcttcggcaa
     1561 actcggcctt cccgacgaac aattgcagtc aatcgcgggc ggcctcatcc agaagatacc
     1621 cctcgggcgt ttcggccatg ctgacgagat cgccaaggcg gtcttgttcc tggcgagcga
     1681 caacgcttcc ttcatcaccg gcgttgaact ggtggccgat ggcggctgga cggagatcat
     1741 gccgtgaccg cgggcgctac gccaatcggt gtccttatcg ccggggtcgg ccctggcctt
     1801 ggtctggcgc tcgcgcgttg ctttgcgcga gcgggccaca atgtggcgat gctcgcgcgt
     1861 cagggcgacc gactcgggga gttcgcgagc ctcgaccgtg accatcttgt cccgatcgcg
     1921 tgcgatctca ccgacccgac agccgtcacg gcggcattcg agacggccga aaaaaagaac
     1981 ggaccgctgg aatgcgccgt tttcaacgcc ggcacattcc gtcccggctc catcctcgac
     2041 acgacgccgg aggatttcga gcactgttgg cggatcggc
//
LOCUS       DATMGX010001029         2463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_348931, whole genome shotgun sequence.
ACCESSION   DATMGX010001029 DATMGX010000000
VERSION     DATMGX010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348931"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VNR65_16895"
     CDS             <1..854
                     /locus_tag="VNR65_16895"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWJ20396.1"
                     /translation="AADVPGPRMLVSGPALSITGGHCDNNMLPFEYHAVSDGVADGIA
                     GVQHKVRENIKYGADLIKVCATGGVLSLGDNPQASQYTLDEMKAIVTDAHRLGRKVAA
                     HAHGAEGIRWAAEAGVDSIEHGSYIDDAGIAAMKEHGTYLVPTLYLGDWMIENAGLTR
                     LPPPLLAKAKDVIPAARKNIAHAFASGVKVALGTDAAVYPHGMNGHEFAVMVRLGLTP
                     LQAIQAGTVNAADLLGWSGKVGSLEPGAWADIVAVDGDPVKDVTTLERVKFVMKGGEV
                     VKNEYGK"
     gene            complement(928..1518)
                     /locus_tag="VNR65_16900"
     CDS             complement(928..1518)
                     /locus_tag="VNR65_16900"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWJ20397.1"
                     /translation="MAYYRRSYPKVQRAKQLLAAGAIGKPVLAELTNHMWFDGQGSRS
                     WLVDPAKAGGGPLFDVASHRIDVLNFLFGQPLRVTGQLSNVVHHYPVEDNATVLIEYA
                     GGVRGIIDVRWHSKVVRDECRIRGTDGEMELTPLNGPDLVYPGAHENLPAHANLHYPM
                     LENFVDAVEGKAPLLASGASSYWTDWVTERARRAGS"
     gene            complement(1537..1872)
                     /locus_tag="VNR65_16905"
     CDS             complement(1537..1872)
                     /locus_tag="VNR65_16905"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWJ20398.1"
                     /translation="MKPDRHNWIVIGIGDIAVRRVIPAIQAEPRSRLYGLVTRDPTKA
                     FPYNVRVWRTLDEALSDPEVHAVYVGTPVFLHAPQAIQSLRAGKHVLSEKPMAMNEAE
                     ARTMVQAAE"
     gene            complement(2031..2324)
                     /locus_tag="VNR65_16910"
     CDS             complement(2031..2324)
                     /locus_tag="VNR65_16910"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWJ20399.1"
                     /translation="MAAGLTKTQLIRHLAEKTELPNKTAAAFLEHLADTAIKETKKNG
                     IFVVPGLGRLVKAHRKARVGRNPQTGEPIQIKAKTVVKFRVAKAAKDAIAPKK"
BASE COUNT          475 a          674 c          812 g          502 t
ORIGIN      
        1 acgccgccga cgtcccgggg ccgaggatgc tggtcagcgg gcctgctttg agcatcacgg
       61 gcggacattg cgacaacaac atgctcccgt tcgaatatca cgcggtcagc gatggcgttg
      121 cggacggcat tgcgggtgtg caacacaagg ttcgcgagaa catcaagtac ggtgcggatc
      181 tgatcaaggt gtgcgcaacc ggaggcgtgc tttctttggg ggacaatccg caggcctcgc
      241 aatacacact ggacgagatg aaagcgattg ttaccgatgc gcaccggctc ggccgcaagg
      301 ttgcggcgca tgcgcacggg gcggagggca ttcgctgggc ggcggaggcg ggcgtcgatt
      361 ccatcgagca cggctcttac attgatgatg ccggcatcgc ggcgatgaag gagcacggca
      421 cttatctggt gccgacgctc tatctgggtg actggatgat cgagaatgca gggctgactc
      481 gcctgcctcc tccgctgctg gccaaggcca aggatgtaat tccggctgcg cgcaagaaca
      541 tcgcgcatgc ttttgccagc ggagtgaaag ttgcgcttgg cactgatgcc gcagtgtatc
      601 cgcatggaat gaacggccac gagtttgcgg tgatggtgcg gctgggcctg acgcccctgc
      661 aggccattca agcaggcacg gtcaatgccg cggatctgct gggatggtcg ggcaaggtcg
      721 gaagcttgga gccgggagcg tgggccgaca ttgtggctgt ggatggggat ccggtgaagg
      781 acgttacaac gctagagcgc gtgaagtttg tgatgaaggg cggcgaagtg gtgaaaaatg
      841 agtacggaaa gtgagctcgc ctgacattgc tagtggtgac ggcggcgagg gcgagacgcc
      901 ctcgcgacag ccggcaagat gccggcgcta cgaacctgct cgccgggctc tctccgtgac
      961 ccagtccgtc cagtaggatg aggcgccact cgcgagcagc ggagcctttc cttcgaccgc
     1021 gtccacgaag ttttccagca tgggatagtg caggttggcg tgagcgggga ggttctcgtg
     1081 ggcgccggga tagacgagat cggggccgtt cagcggagtt agttccatct cgccgtcggt
     1141 gccacgaatg cggcattcgt cgcgcaccac tttggaatgc cagcggacgt cgatgatgcc
     1201 gcggactccg ccggcatatt caatcaaaac ggtggcgtta tcctccactg ggtagtgatg
     1261 cacaacgttc gagagttggc ccgtgacccg cagcggctgg ccgaaaagaa aattcaaaac
     1321 atcgatgcgg tgcgaggcga cgtcgaagag agggccgcct ccggctttcg cgggatcgac
     1381 cagccaactg cggctacctt gcccgtcaaa ccacatgtgg ttcgtgagtt cggccagaac
     1441 gggcttgccg atcgcgccgg cggcgagcag ttgcttcgcg cgctgcacct tcgggtaaga
     1501 gcggcgatag taggccacgc cgaaggtctt gccactctat tccgcggctt gaaccatggt
     1561 gcgggcttcg gcctcgttca tggccatggg cttttcgctg agcacgtgct tgcccgcgcg
     1621 gagggactga atcgcctgcg gcgcgtgcaa aaagaccggc gtgccgacgt agacagcgtg
     1681 aacttcagga tcggagagag cttcgtcgag agttcgccag acgcgcacgt tgtaaggaaa
     1741 ggccttcgtt ggatcgcgcg tgaccaggcc gtacaggcgg ctgcggggtt cggcctggat
     1801 tgcggggatg acgcgtcgca cggcgatatc gccgatgccg atgacgatcc agttgtggcg
     1861 gtcaggtttc aaggtttcaa agttgcaagg tttcgcggca tcgaatcact gccggttgga
     1921 accttgcaac cttgaaaccc tgaaactttg aaacctgtcg ttccagaacc tgtactccag
     1981 aaaaccaaag gccggcttga cgccggcctt tgcatgatct cacgcaacaa ctacttcttg
     2041 ggggcgatcg cgtccttcgc ggctttggcg acgcggaact tcacgacggt cttagccttg
     2101 atctggatgg gttcgccggt ctgcgggtta cgtcccacgc gagccttgcg gtgcgccttc
     2161 accagacggc cgaggccggg cacaacgaaa atgccgttct tcttggtttc tttgatagcg
     2221 gtgtcggcca ggtgctccag gaacgccgcc gcggtcttgt tgggaagctc ggtcttctcg
     2281 gcgagatgcc gaatgagctg tgtttttgtc aaacctgcag ccatgtaatt cctcctcaga
     2341 aaaatcggtt taataccagc ggggcctatc ttatacccgc gggcctgtgg aaaacacctt
     2401 attttacagg ggttttctaa tttgccaacg cggaagaggc catttctaca gggctttcta
     2461 tat
//
LOCUS       DATMGX010001030         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_57109, whole genome shotgun sequence.
ACCESSION   DATMGX010001030 DATMGX010000000
VERSION     DATMGX010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57109"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VNR65_16915"
     CDS             <1..935
                     /locus_tag="VNR65_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ20400.1"
                     /translation="LRLRVVGQDQALERVANAIRRSRAGLSDPKRPIGSFIFLGPTGV
                     GKTELARALAEFLFDDEHALLRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEQVR
                     RRPYAVVLFDEIEKAHPDVFNILLQMMDDGRLTDGKGRVVDFKNTIITMTSNIGSSYL
                     QSDHVRTPEEFEKASEQVLSLLHTHFKPEFLNRVDDIIVFGPLGKEQLVKIIELRLED
                     VRRLLADRKISLELTDAAKELLFTQGHDPNFGARPLKRSIQKLVQDPLALKILDGEVL
                     HGDHIIVDADKKAGKMTFEVSPRVGEKAPAKTKK"
     gene            1063..1833
                     /locus_tag="VNR65_16920"
     CDS             1063..1833
                     /locus_tag="VNR65_16920"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HWJ20401.1"
                     /translation="MTRTLSSFAAVILISFVQLSVAQSPMKWKIHDLNRPLPPIVDPG
                     TASTQDAPGRAPSDAVVLFDGKDLSRWVDSDGKPARWKVENGYMEVAAKTGNISTRDS
                     FGDCQLHVEFAEPSPGKGESQERGNSGVFLMSTYEIQVLDSYENKTYADGQASAVYGQ
                     YPPQVNASRPPAQWQTYDIIFHRPHFMDGKVTQPARVTVLHNGVLVQDNVELTGPTAH
                     GERPPYKPGPDKLPLQLQDHGNPVRYRNIWIRELQEGQ"
     gene            complement(1883..>2121)
                     /locus_tag="VNR65_16925"
     CDS             complement(1883..>2121)
                     /locus_tag="VNR65_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20402.1"
                     /translation="GYALVGWGLFRLHNWARWAAMLVMVVAVASLVPKISMAELGVQV
                     LWYGLQIALRAAVAWYLAQAPAVIDAFAGKTPAL"
BASE COUNT          468 a          695 c          586 g          372 t
ORIGIN      
        1 gcctgcgcct gcgcgtcgtc ggccaggacc aggctctgga gcgcgtggcc aatgccattc
       61 gccgctcgcg cgctggactc agcgatccca agcgtcccat cggctcattc atcttcttag
      121 gcccaacggg cgtgggcaag accgaactcg cccgcgcgct cgccgaattt ctgttcgacg
      181 acgaacacgc cttgctccgc atcgatatgt cggagtacat ggagaagcac tcggtctctc
      241 gcctgatcgg cgcgcctccg ggctatgttg gttacgaaga aggcgggcag ctcaccgagc
      301 aggttcgccg ccgtccctat gcggtcgtgc tcttcgatga aatcgaaaag gcgcatcccg
      361 acgttttcaa cattcttctc cagatgatgg atgatggccg attgaccgac ggcaaaggcc
      421 gcgtcgtcga cttcaagaac accatcatca ccatgacgtc gaacatcggc tccagctatt
      481 tgcagtcgga ccatgtgcgc acgccggagg aattcgaaaa ggccagcgag caggttctga
      541 gcctgctgca cacgcacttc aagcccgagt tcctcaaccg cgtcgacgac atcatcgtct
      601 tcggcccgct gggcaaggag cagttggtca agatcatcga actgcgtctg gaagacgttc
      661 gccgcctgct ggccgaccgc aagatctcgc tcgaactcac cgacgcggcg aaagaactgc
      721 tattcacgca aggccatgat ccaaacttcg gcgcgcgccc gctgaagcgc tcgatccaga
      781 agttggtgca ggatccactg gcgctgaaga tcctcgacgg tgaagtcctc cacggcgatc
      841 acatcatcgt cgacgccgac aagaaggccg gcaagatgac gttcgaggtc agccctcgcg
      901 tcggcgagaa ggcgccagcc aagaccaaga agtgaaaggc gccacggatt gtacagattg
      961 acacggatca agtccgactc acctgggctt ggtccgcgcg aatccgtgtt gatccgtggc
     1021 aaaatttcgg tatcatgttc tccggcgcgc gaggcgccgc tcatgacccg aacactttcg
     1081 tcattcgcag cagtcatcct gatttcgttt gtccaactct ctgttgccca aagtcccatg
     1141 aaatggaaaa tccacgacct caatcgccca ttgccgccaa ttgtcgatcc cggcaccgcc
     1201 agcacgcagg acgctcccgg acgcgctccg tcggacgctg tcgtcctttt tgacggcaaa
     1261 gacctgtcgc gctgggtcga cagcgacggc aagcctgcaa gatggaaagt cgaaaacggc
     1321 tacatggaag tcgcagccaa gaccggcaac atttctacgc gcgactcctt tggagactgc
     1381 cagctgcacg tggaattcgc cgagccttca cccggcaagg gagagagcca ggagcgcggc
     1441 aacagcggcg ttttcctaat gagcacctat gagatccagg tactcgactc gtacgagaac
     1501 aagacctacg ccgacggaca agcatcagcg gtctacggac aatatccgcc gcaggtaaat
     1561 gcttcgcgcc ctccggcgca gtggcagacc tacgacatca tctttcaccg cccgcacttc
     1621 atggatggca aggtcacgca acccgcacgc gtgaccgttc tgcacaatgg cgtgctcgtg
     1681 caggacaacg tagaactcac cggaccaacc gcgcacggcg agcgtcctcc ttacaaaccg
     1741 ggacccgaca agcttccctt gcagttgcag gaccacggca atcccgtgcg ctaccgcaac
     1801 atctggattc gcgaactgca agaagggcag tgagagaatc gcgtagcggc ggacgcattc
     1861 gtccgctcgt aagaggtatt ccctacagcg caggcgtctt cccggcgaac gcgtcgatca
     1921 ctgcgggcgc ttgcgccaga taccacgcaa ccgccgcgcg cagcgcgatc tgcaatccgt
     1981 accagagcac ctgaaccccg agttccgcca tcgaaatctt cgggacgagc gatgccacgg
     2041 cgaccaccat caccagcatc gccgcccagc gcgcccaatt gtgtagccgg aacaatcccc
     2101 aaccgacgag cgcgtaacca g
//
LOCUS       DATMGX010001031         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_147738, whole genome shotgun sequence.
ACCESSION   DATMGX010001031 DATMGX010000000
VERSION     DATMGX010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147738"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VNR65_16930"
     CDS             complement(<1..538)
                     /locus_tag="VNR65_16930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWJ20403.1"
                     /translation="MPDFLVTVVIPTLAADSRLLECVQSLSRQTMADLEVVIVDNSGH
                     GLVRRNGTAPGARVIENPRNAGFGTAINQGWRASTSPYVATLNDDAVAHPGWLAALVS
                     ALDARPDAGVCASQVRLFGEPRLDSAGMLVARDGSSKQRGHGRLPEDFPVLEEVLMAS
                     GSAALYRRTMLDDTGGFDD"
     gene            675..1937
                     /locus_tag="VNR65_16935"
     CDS             675..1937
                     /locus_tag="VNR65_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20404.1"
                     /translation="MHVHMQTAETLNPDQISQFLKLGDTIEFAGQSRAERYAFAQQLL
                     VAQEYARQGKKQRGAIRAYLSKMTGLGVAQTARLIQMYIATGAVELRGYRRHEFVSKY
                     TDGDVRLLATVDRAHERLSGPATCCILRREYERFGKAEYARLAQISVSHLYNLRNGTL
                     YRKHAAVYLPTRPTAVSIGERRKPDPQGRPGFLRIDTVHQGDWDGNKGVYHINAVDTV
                     TQWQVVGCASKINEAFLLPVLEAILHQFPFVILGLHADNGSEYINYTVANLLEKMLAE
                     FTKSRANRSQDNALVEGKNGAVVRKHIGYGYIGGEHAERLQKFYTAHFNPYLNFHRPC
                     GFATVSLDARGKRKREYKAEDYRTPYEKLKAMPEAAKHLKQNISLADLEQRAMQMSDT
                     ECARKMTTVKAKLLRDCKTESLLPPRFL"
BASE COUNT          512 a          668 c          761 g          375 t
ORIGIN      
        1 tgtcgtcgaa gccgccggtg tcgtccagca tggtacgccg gtagagcgcc gccgagccgc
       61 tggccatgag gacttcctcg agcactggaa aatcctcggg gaggcggccg tggccgcgct
      121 gcttgctaga gccgtcgcgc gccaccagca tgccggcgga atccaggcgg ggctcgccga
      181 agaggcgcac ctgggaagcg cacacgccgg cgtcggggcg ggcgtcaagg gcggagacga
      241 gcgcggcgag ccaacccgga tgagccacgg catcgtcgtt gagggtggcg acataggggg
      301 acgtggaagc gcgccagccc tgattgatgg ccgtgccgaa gccggcattg cgcggattct
      361 caatcacgcg cgcgcctggc gcggtgccat tgcggcgcac caggccgtgg ccgctattgt
      421 cgacgatcac cacttcgaga tccgccatgg tctgcctgga cagcgattgc acgcactcga
      481 gaagcctcga atccgcggcg agggtgggga ttaccaccgt tacgaggaag tcaggcaatt
      541 cttgattcta ccttgtagag gggggtgggg gtggcagaga attgtccaac gcaagatgat
      601 actgaaacgc gacttgattc caatgcaaga tgatactgaa aatggaggtg gagtgacggg
      661 cgagctgcga ccgaatgcac gtacacatgc agaccgccga gacattgaat cccgatcaga
      721 tcagccagtt tttgaaactg ggcgacacca tcgaatttgc cgggcagagc cgcgccgaaa
      781 ggtacgcgtt tgcgcaacag ttgctggtcg cgcaagagta tgcaaggcaa ggcaagaagc
      841 agcgcggcgc catccgcgcc tacctgagca aaatgaccgg attgggcgtg gcgcagaccg
      901 cgcggctgat ccagatgtac atcgcaaccg gagccgtcga gttgcgaggg taccggcgcc
      961 acgagttcgt cagcaagtac accgacggcg atgtgcgcct gctggcaacg gtggatcgcg
     1021 cccacgagcg attgagcggg ccggcgacct gctgcattct acggcgggag tacgagcggt
     1081 tcggcaaggc ggaatacgcg cggttggcgc agatctcggt atcgcacctg tacaatctgc
     1141 gcaacgggac gctgtaccgc aagcatgccg ccgtgtacct gcccacgcgg ccgacagcag
     1201 tatccatcgg ggaacggcgc aagccggacc cgcaggggcg gccgggcttt ctgcgcatcg
     1261 atacggtgca tcagggggac tgggacggca acaagggggt gtatcacatc aatgcggtgg
     1321 atacggtcac acagtggcag gtggtgggat gcgcgagcaa gatcaacgaa gcgtttctgc
     1381 tcccggtgct ggaagcgatc ctgcatcagt ttccctttgt gatcctgggc ctgcacgccg
     1441 acaacggctc ggagtacatc aactacacgg tggcgaatct gttggagaaa atgctggcgg
     1501 agttcaccaa gagccgggcc aatcgcagcc aggacaatgc gctggtggaa ggcaagaacg
     1561 gtgcggtggt acgcaagcat atcgggtacg gttacattgg cggcgagcat gccgagcggc
     1621 tccagaagtt ctacacggcg cacttcaacc catacttgaa cttccatcgt ccgtgcgggt
     1681 ttgcgacggt cagcctggat gcacgcggca agcgcaaacg ggagtacaag gcggaggact
     1741 accggacgcc ctacgagaag ctgaaggcca tgccggaggc ggcgaaacac ttaaagcaga
     1801 acatcagctt ggcggacttg gagcagcggg cgatgcaaat gagcgatacc gagtgtgccc
     1861 gcaaaatgac gaccgtcaaa gcgaaattgc tgcgcgactg caagacggaa tcactgctgc
     1921 cgccgaggtt tctatgaagg cgccacaaga gggccgtgga aatgacgggg cggtggaaag
     1981 cgtggaaaac cacgaacagg tttccccctc ttcccaccgc cccttggaaa tctcgcaagg
     2041 acggcgagat tcccacattc ccaccgccag gcgtcgcccc gcgggagagc gtggaaaacc
     2101 aaagagcggt ttcccacact cccccgcgac gctcgcgacg gcgactgcga gtccctcttc
     2161 cgaacagcaa aaccaaaccc tcgggcgttc cgcccgaaag acaggagaaa ggccacgcta
     2221 gccgttcatc cgccgctgcc ttttcagtat caccttgcgt tggaaacgaa agtcgatttc
     2281 agtatcattc ttcgattgga aaatgctgtg cacgct
//
LOCUS       DATMGX010001032         3214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_78284, whole genome shotgun sequence.
ACCESSION   DATMGX010001032 DATMGX010000000
VERSION     DATMGX010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78284"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VNR65_16940"
     CDS             complement(<1..427)
                     /locus_tag="VNR65_16940"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF021908.4"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HWJ20405.1"
                     /translation="MAKPKILERLTPVAAVDRLEDLYAKAASSLAEALDTYLSTSVPP
                     SPETRAKFRYPLLRLVYRNEESPRSPSLRAFARLHAQGVYETTVTHPAVFRSYLLEQL
                     EPLALEYGADIEVGVSGQEIPYPYVIERAEEITGANVSAS"
     gene            925..1611
                     /locus_tag="VNR65_16945"
     CDS             925..1611
                     /locus_tag="VNR65_16945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ20406.1"
                     /translation="MNSEAAIKPGETVTWMGLAFETSDQVLVPRPETELLARTALDLL
                     KTVESAAPIRVIDMCCGAGNLACALAHARPDIRVWASDLTDACVGLARKNVARHGLEG
                     RVDVRQGDLFSSLPDEELVGFVDLVVCNPPYISTGKLESESAHLLDGQPREAFDAGPY
                     GLAIHQRVIADAPRFLKPGAWLVMEIGLGQQRQIALLFSRVKRYEKEQSWSDENGAPR
                     VMGARISSAS"
     gene            complement(1664..2365)
                     /locus_tag="VNR65_16950"
     CDS             complement(1664..2365)
                     /locus_tag="VNR65_16950"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWJ20407.1"
                     /translation="MAQSAEQPELNVVPLQTSSSLRTLAYGALKRAITEMDIYGQPDL
                     IRLDERQLSQKLGVSRTPIREALTLLEQEGFVRAVPRRGIYVVRKSKKEICEMIVVWA
                     ALEGMAARLAATRATEEQLNALADLFQKFKDEPLSDQMNEYSEANIAFHQAIIKLGGC
                     DLIVETTRNLFIHIRAMRTVSLRQDNRAEQSIGEHLAIIGALKARDADLAEKLVREHS
                     LGLAALVEKQGVFAD"
     gene            2813..>3214
                     /locus_tag="VNR65_16955"
     CDS             2813..>3214
                     /locus_tag="VNR65_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20408.1"
                     /translation="MNLEALRFSKGKCPARLNGVKKMDESVTAFAEKLRAHSNALGES
                     ADIEWRLSQTPVAYESAIVTMEDRASAIFSGNASELIWFLEHPALYTAGASAKPADLL
                     DPARLPVHHTGRGGQYTYHGPGQLVAYVLLDL"
BASE COUNT          649 a          938 c          971 g          656 t
ORIGIN      
        1 cgctcgccga gacattcgcg cctgtgatct cctccgcccg ttcgatgacg taagggtagg
       61 ggatttcctg accgctcacg ccgacctcga tgtcggcgcc gtactccagc gcgagcggct
      121 caagctgctc aaggaggtag cttcggaaaa cagccggatg ggtcaccgtc gtctcgtaga
      181 cgccctgggc gtggagcctg gcaaaggcgc gaaggcttgg cgagcgaggc gactcttcgt
      241 tgcggtaaac gagccggagt aaaggatagc ggaacttggc gcgggtttcc ggcgagggag
      301 gaacgcttgt gctgagatag gtgtcgagcg cttccgcaag cgaggaagcg gcctttgcgt
      361 ataagtcttc gagccggtct accgcagcaa caggtgtaag acgctctaaa attttgggct
      421 ttgccatgac atgccgatcg cgattgaaaa aaggagaaag accgcggaag gtcggcttac
      481 cacgtaaggg gcaacatagt cctgtttcta gatcaacccg cctttaatcg gaatcgatta
      541 aaggcggaaa gttgcacgga cacattgatc cgaagaactg gtcgcccggc tggctgctct
      601 ggccgagttc cttcgtcgaa atgtcgaaag ccttagccgt gaacgccttg ttgatcgtga
      661 tatcgatgct tccgagccag gcgccctggg ttgaccgctc gccttcgcct cgcctgatgc
      721 gatgaggcga tgcgttttct ccgaggtgat catgacttca gctcttcaat agccggaagg
      781 ctctctgcag aacgcaccct gagagacgcc tctccacacc aattacgtgc atttgcgaac
      841 gacacgctgg cgggcgttct ggcgactcag ctactgctcg tcccggcgaa cgtcagcgtg
      901 gcaatcccga acacggagcc tttcatgaac tcggaggcgg ctatcaagcc cggcgagact
      961 gtcacctgga tggggctcgc cttcgaaacc agcgaccagg tgctcgttcc gcggccggag
     1021 accgaacttc tggcacgcac cgcgctcgac cttctcaaga ccgtcgagtc ggcggcgccg
     1081 atccgcgtca tcgacatgtg ctgcggagcg ggcaatctcg cctgcgcgct cgctcatgcg
     1141 cggccagata tccgcgtctg ggcgagcgat ctcacggatg cgtgcgtcgg gctcgcgcgg
     1201 aagaacgttg cgcggcacgg cctcgaaggg cgcgtcgacg ttcgccaggg cgatctcttt
     1261 tcgtcgcttc ctgacgagga actcgtaggc ttcgtcgatc ttgtcgtttg caatccgccg
     1321 tacatttcga cggggaagct tgagagcgaa agcgcccacc ttctcgacgg tcagccgcgg
     1381 gaggccttcg acgccggtcc ttacggcctc gcgatccacc agcgggtgat cgcggacgcc
     1441 ccacggttcc tgaagcccgg agcctggctc gtcatggaga tcgggctcgg ccaacagcgt
     1501 cagatcgcgc tcctcttctc tcgcgtgaag cgctatgaga aggagcaatc ctggtcggat
     1561 gagaacggcg cgccacgcgt aatgggagcg cgtatatcga gcgcgagtta gcggaacggt
     1621 tcggagagaa gccagatcac cgtcccacgg cgtcgtcctt gcgctagtct gcgaaaacgc
     1681 cttgcttttc cacgagggcg gcaagtccaa gcgagtgctc ccgcacgagc ttctcggcaa
     1741 ggtcggcgtc gcgggccttg agcgcgccga taatcgccaa atgctcgccg atggactgct
     1801 cggcacggtt atcttgcctc agcgacaccg tgcgcatagc ccgaatatga atgaacaggt
     1861 tccgggtcgt ctccacgatg aggtcgcagc cgccgagctt gatgatcgcc tgatggaagg
     1921 cgatgttggc ttccgaatat tcgttcatct gatcggacag cggctcatcc ttgaacttct
     1981 ggaaaagatc cgcgagcgcg ttgagctgtt cctccgtcgc gcgcgtcgcc gccagcctcg
     2041 cggccatgcc ttcgagcgcg gcccaaacca cgatcatctc gcagatttct tttttcgact
     2101 tgcgcacgac gtagatgccg cgtcgaggga cagcacggac gaaaccctcc tgttccagga
     2161 gcgtaagcgc ctcccgaata ggggtccggc tgacgccgag cttctgcgag agctggcgtt
     2221 catccaaccg gatcagatcg ggctggccgt agatatccat ttcagtaatt gcacgcttca
     2281 gagcgccata agccagggtt ctgaggctag agctggtttg aagagggacg acattgagct
     2341 ccggctgctc tgccgactgt gccacgctat actctccatt gttgactccg ctcgttcagc
     2401 gagggagccg caaaaatttt tctacacatc caagccagcc gtgcggggaa atgcattcaa
     2461 caatcgatta cggatatata tataggaaaa gagcgaactt taccgacggc ttacgatggc
     2521 ctcttgaata aattcgaaag acccttggtg cactaaatac caagaggata attgtatcat
     2581 ttgcatttca tagagttttg atttgaatca gttcaacgcg tgctcgggcg aaaaaacttc
     2641 cccatgcgag cgcaccgtgt agcgtcgccg cgtcatgttc tgaatttctc ttgtcgatat
     2701 gggattttgg gtctcgccgc gacggcttca gagccgagct gttgcactgc ggggcgtcgg
     2761 ggcggacgcg ggacagcgcc ccaaccccgc agcaacgcga gacctttgca gtatgaacct
     2821 cgaggcgtta cgcttcagta aagggaaatg ccccgcaagg ttaaacggcg taaaaaaaat
     2881 ggatgagagc gtcaccgcct tcgccgaaaa gctgcgcgcc cattcgaacg ctctggggga
     2941 gagcgcagac attgagtggc gattgagtca gacaccggtt gcgtatgaat cggcaatcgt
     3001 gacgatggaa gatcgcgcct cagccatttt ttctggaaat gcctcggagc tcatctggtt
     3061 tctggagcac ccggcgcttt acacggcggg cgcgagcgcg aagccggcgg atctgctgga
     3121 cccggctcgc ttgccagtcc accacacggg acggggaggg cagtacactt atcacggccc
     3181 ggggcagctc gtggcctatg tcctcctcga tctc
//
LOCUS       DATMGX010001033         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_338293, whole genome shotgun sequence.
ACCESSION   DATMGX010001033 DATMGX010000000
VERSION     DATMGX010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338293"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VNR65_16960"
     CDS             complement(<1..547)
                     /locus_tag="VNR65_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWJ20409.1"
                     /translation="MAKQKFERTKPHCNVGTIGHIDHGKTTLTAAITKVLQKHNPTIV
                     FRSFDSIDNAPEEKARGITIATAHVEYETANRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQTREHVLLARQVGVPYIVVALNKCDAVDDPELLDLVELEVRELLK
                     SYQYPGDDVPVVRLSALGALNG"
     gene            complement(568..873)
                     /locus_tag="VNR65_16965"
     CDS             complement(568..873)
                     /locus_tag="VNR65_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20410.1"
                     /translation="MDGRQADFDAVFGPGGIWSELLRRAEGYAGTEVWSESSTERCYR
                     VRDFWWSHREFEAFRVRFVVEIERFHSLLMSEGIVSKFEVVGTYYDERGDGDDLVAT"
     gene            complement(916..>2750)
                     /gene="fusA"
                     /locus_tag="VNR65_16970"
     CDS             complement(916..>2750)
                     /gene="fusA"
                     /locus_tag="VNR65_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWJ20411.1"
                     /translation="FTAEVERSLRVLDGAVAVFDAVHGVQPQSEKVWRQADKYGVPRI
                     CFINKIDKMGADFEHAVDTLRKRLNAKPVAIQIPIGQEDKFRGVVDLVAMKSIIWPDD
                     TIGAEYQVGDIPADMQKKADAFHTQLVETVAENDDEILHKFLEGEPISADELKASLRK
                     STIGLKLFPVVVGTAFKNKGVQPLLDAVVDYLPSPLDTKETHGLDPDNGKTITRKADD
                     KDPFAALAFKIMADPFVGQLTFIRVYSGQLKSGESVMNSRTQKHERIGRLLKMHANKR
                     EDIQEILAGDICAVVGLRNVSTGDTICEEKHPITLESIVFAVPVISVAVEPKTKADQE
                     KMGMALARLAQEDPTFKVHTDPDSGQTIISGMGELHLEIIVDRMMREYKVEANVGKPQ
                     VAYRETIRKHAEAEGKYIRQTGGRGQYGHAKIYLDPNEPGKGYEFINDIVGGTVPKEY
                     IKPIDQGMQEAMEGGVLAGYPMVDIKATLYDGSYHDVDSNEMAFKIAGSMAFKEAARK
                     ASPVILEPVMAVEVVTPEDYAGAIMGDLSSRRGRIEGMEHRAGSQVIKAIVPLAEMFG
                     YATHMRSSTQGRAEYSMHFARYEEAPRHVADEIIAKTQGKTAPR"
BASE COUNT          525 a          903 c          702 g          620 t
ORIGIN      
        1 cgccgttcaa cgcacccaga gcggagagtc gcaccacggg cacatcgtcg ccaggatact
       61 gatagctctt caacaactcg cgaacttcga gctcgaccag atccagcaac tcgggatcgt
      121 ccaccgcatc gcacttgttc agcgcaacca cgatgtaagg cacacctacc tggcgcgcca
      181 gcagcacgtg ctcgcgtgtc tgcggcatcg gaccatccgt cgccgccacg actaagatcg
      241 cgccgtccat ctgcgccgca cccgtgatca tgttcttgat gtaatcggcg tgacctgggc
      301 agtcgacgtg cgcgtagtgc cggttcgccg tctcatattc cacgtgcgcc gtggcaatgg
      361 ttatgccgcg cgccttttct tccggagcgt tgtcgatcga atcgaacgac cggaaaacaa
      421 tggtcgggtt gtgtttctgc aacaccttcg tgatcgccgc cgtcaacgtc gtcttcccat
      481 ggtcgatgtg accaatcgtc cccacgttgc agtgcggctt tgtccgttca aacttttgtt
      541 tcgccatttg ctcttagctc ctcagctcta cgtcgcgact aaatcgtcac cgtcaccacg
      601 ctcgtcgtag taagtaccga cgacttcaaa cttgctcacg atcccttcgc tcatcagcag
      661 cgaatgaaac cgttctatct ctaccacaaa ccgtacccgg aacgcttcga actcgcgatg
      721 cgaccaccaa aaatcgcgca cccgatagca ccgttccgtc gaggactccg accatacctc
      781 tgtccccgca taaccttcgg ctcggcgaag aagctcactc caaatccctc ccggcccgaa
      841 caccgcatca aaatctgcct gccggccatc catcgcccga aatctgcgat cggcgacgaa
      901 ccgcagtccg tgctgtcatc taggcgccgt ctttccctgc gtcttggcga taatctcatc
      961 cgccacgtga cgcggggctt cctcataacg cgcaaaatgc atcgagtact cggcgcgtcc
     1021 ctgggtcgac gaacgcatat gagtcgcgta cccaaacatc tccgctagcg gaacgatagc
     1081 cttgatcacc tgcgaaccgg cgcggtgctc catgccttcg atccggccgc gacgggagct
     1141 caggtcaccc atgatcgctc cggcgtaatc ctccggcgtc actacttcga ccgccatcac
     1201 cggctccaga atcaccggac tcgccttgcg cgccgcctct ttgaaggcca tcgaaccggc
     1261 aatcttgaat gccatttcgt tcgaatcgac atcgtgatag ctgccgtcgt aaagcgttgc
     1321 cttgatatcg accatcggat agccagcaag aacgccgcct tccatggctt cctgcatgcc
     1381 ctggtcgatc ggcttgatgt attccttcgg caccgtgccg ccgacgatgt cgttgatgaa
     1441 ttcgtagccc ttgcccggct cgttcggatc gagataaatc ttcgcgtgcc cgtactggcc
     1501 gcggccgccc gtctgccgga tgtacttgcc ttccgcttcc gcgtgcttgc gaatggtctc
     1561 gcgatacgcc acttgcggct tgccgacgtt ggcttcaacc ttgtattcgc gcatcatgcg
     1621 gtcgacgatg atttcgagat gcaactcgcc catgccgctg atgatggtct gtccgctatc
     1681 gggatcggtg tgaaccttga aggtaggatc ttcctgcgcc agtcgcgcca gcgccatgcc
     1741 catcttttcc tgatcggcct tggtcttcgg ctcgacagcc accgaaatca ccggtaccgc
     1801 aaagacgatc gactccagcg tgatcggatg cttctcttcg caaatcgtgt cacccgtact
     1861 gacgttcctg aggcctacga cagcgcagat atcaccggca agaatttcct ggatgtcttc
     1921 gcgcttgttg gcgtgcatct tgagcagacg cccaatgcgc tcatgtttct gcgttcgcga
     1981 gttcatcacc gactcgcccg acttcaactg tcccgagtag acgcggataa aagtcagctg
     2041 cccgacaaac gggtcggcca tgatcttgaa ggccagcgcc gcgaaagggt ctttgtcgtc
     2101 ggctttgcgc gtgatcgtct ttccgttgtc gggatcgagg ccatgcgtct ctttggtgtc
     2161 gagcggcgag ggcaggtaat ccaccaccgc atccagcaac ggctgcacac ccttattctt
     2221 gaacgccgtc cccaccacca ccggaaacaa tttcagcccg atcgtggatt tgcgcagcga
     2281 cgccttaagt tcgtccgccg aaatcggctc gccttccaga aacttgtgca gaatctcgtc
     2341 atcattctcg gccacggttt cgacgagttg cgtgtggaaa gcatcggcct tcttctgcat
     2401 gtccgcggga atgtcgccca cctggtactc cgcgccaatt gtgtcatctg gccagatgat
     2461 ggacttcatg gcaaccaggt ccaccacgcc gcgaaacttg tcttcttgtc cgatgggaat
     2521 ctgaatcgcg actggcttgg cgttgaggcg cttgcgaaga gtatcgacgg catgctcgaa
     2581 gtcggcaccc atcttgtcga ttttgttaat gaaacaaatt cgcggaacgc cgtatttatc
     2641 cgcttgccgc cagacttttt ccgactgcgg ctggacgcca tgcaccgcgt caaacacggc
     2701 gacggcgccg tccagcacgc gcagcgatcg ctccacttcg gccgtaaagt
//
LOCUS       DATMGX010001034         3103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_119100, whole genome shotgun sequence.
ACCESSION   DATMGX010001034 DATMGX010000000
VERSION     DATMGX010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119100"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VNR65_16975"
     CDS             <1..187
                     /locus_tag="VNR65_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HWJ20412.1"
                     /translation="VELIGATTTKTGLKVECLLDTRTYEKGIKISDAEMEMLNIAGDG
                     FHPEWNYTIKPRLLPNS"
     gene            complement(151..588)
                     /locus_tag="VNR65_16980"
     CDS             complement(151..588)
                     /locus_tag="VNR65_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20413.1"
                     /translation="MTYRSLLNEDWPAIIERLGGTAALDESARNAKAFLRARTFENAI
                     DMLRMILAYCLGGGLRSTAAWGASIGLADVANTALLYRLRQSGDWLAWLVGQALGSAA
                     PKASQGRPICIVDATNVLKAGVAAKDAPNNNRYEFGRSLGLMV"
     gene            complement(639..893)
                     /locus_tag="VNR65_16985"
     CDS             complement(639..893)
                     /locus_tag="VNR65_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20414.1"
                     /translation="MSPSGMTLEREDRLAHHLGPILIAGEGHTLLATGAKQGQSRGGE
                     FETLFFDRQRVQRSLAELLRGQEDLKRLAPQGVAALDVVP"
     gene            892..1191
                     /locus_tag="VNR65_16990"
     CDS             892..1191
                     /locus_tag="VNR65_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20415.1"
                     /translation="MLFRLRETKANEPKLRVLRCLIDGAEELFGDEPFLASDLYNRGD
                     ELDELADTLAQMGFTNTPELGVYLSGNRGAPIDGRWFEDTGKRQQRSVLWRVSRK"
     gene            complement(1410..2351)
                     /locus_tag="VNR65_16995"
     CDS             complement(1410..2351)
                     /locus_tag="VNR65_16995"
                     /inference="COORDINATES: protein motif:HMM:NF024151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20416.1"
                     /translation="MFGLFVLIILVFAGGAEAAGRYNADGPYAVTTAVLTVPSPNGAF
                     TTTAYIPKDSRPHPVVIFSSGFMQSGIAYEPYAKRLASWGIIAFLQDDATLAYAPAQA
                     KFPDEPNDNPTITEMAVNAVSNVSHQVSTWLPAINADGPLHGMVDVSRIGLAGHSRGA
                     QIALLAAEGSPGSVKGVFGLDPVDLSFGTAKAGAQLANIGIPVAFIGETVNKLSCAPA
                     GFNYQALYDVAASPAVAITALKADHTMFQDPANCHFCSLCTKGTANPSTVLAFSVRYI
                     TAFFARELLSDARVGAVFEGAGAAADVKAGLIQIASK"
     gene            2534..2923
                     /locus_tag="VNR65_17000"
     CDS             2534..2923
                     /locus_tag="VNR65_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20417.1"
                     /translation="MPIGSLDVSRPPLRALAVTPDLSQLPDLTAAQLVTEFTREARRT
                     PIDKTKHPSASAVVVRTCAVGIDYVSVGDCTLLVEGTIGFVRVGVDDAGDPQLAQALA
                     ALHAKHSGLDAKTAQAHIWPSIKPGQL"
     gene            2920..>3103
                     /locus_tag="VNR65_17005"
     CDS             2920..>3103
                     /locus_tag="VNR65_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20418.1"
                     /translation="MNELYGVFSITPTPRYFVQAGQIATPAASVHALLASDGLMRLVD
                     VFGLYTADELFAAARNE"
BASE COUNT          647 a          921 c          927 g          608 t
ORIGIN      
        1 cgtcgagctg atcggtgcga cgacgaccaa aaccggatta aaagttgagt gtctgctcga
       61 tacgcgcacc tacgaaaagg gcatcaagat cagcgacgcc gagatggaaa tgctcaacat
      121 cgcaggcgat ggcttccatc ccgaatggaa ctacaccatt aagccgaggc tcctgccaaa
      181 ttcgtagcgg ttattgttcg gagcgtcctt agcggcgacc ccagctttca gaacattggt
      241 ggcgtcgacg atgcagatcg gccgcccctg gctcgctttg ggcgcggcgg aaccgagcgc
      301 ttgccccacc agccaggcaa gccaatcccc gctttggcgc aggcggtaga gcaatgcagt
      361 attggcgaca tcggcaagcc caatcgaggc gccccaggcg gcggtggacc gcaagccgcc
      421 gcccaggcaa taagcaagga tcatgcgcag catgtcgatg gcattttcga aagtccgagc
      481 gcgcagaaaa gctttggcgt tccgggcgct ctcgtcgagc gccgccgtcc cgccaagccg
      541 ctcgattatg gctggccagt cttcattcaa aagcgatctg taggtcatgc catgctggaa
      601 tcagactcga ttctacctgg caagccctat tttagcgcct atgggacaac atccaaagcg
      661 gctacgcctt gaggagcgag gcgtttgagg tcttcctgac ctcggaggag ttcagcgagc
      721 gaccgctgca cacgttgacg atcgaaaaac aacgtttcga actcgccgcc gcgggattga
      781 ccttgcttcg cgccagtcgc gagaagtgtg tgtccttctc ccgctatgag gattggacca
      841 aggtggtgcg ccagccgatc ctcgcgctcc aacgtcatcc ccgaaggcga catgctgttc
      901 cgcttgcggg agacgaaagc gaacgagccc aaattgaggg tcttgcgctg cctcatcgac
      961 ggtgccgaag aactgttcgg cgacgagccc ttccttgctt cggacctcta taacaggggt
     1021 gatgagcttg atgaactggc agatactctg gctcaaatgg gctttaccaa taccccggaa
     1081 cttggcgtgt acctgagcgg caatcgcggc gcaccgatcg acggacgctg gttcgaggac
     1141 acaggcaagc ggcagcagcg atcagtcctg tggcgagtga gtcggaagtg agtcaaaagt
     1201 gagtctagaa aagtgttaac ctgctcatag agccctcatt tgactcacaa gagttctcag
     1261 tttttgaccc acttaactca ctcgctgctc accacgctca acagctgcac ctctcctcgc
     1321 gctttcagac cacctgcttt ttatcggggc gggcgggcaa ggggggctgt tgcggtcggg
     1381 gagggcgtcg tggcatccgc tgcagcgatc tactttgagg cgatctggat gagcccggct
     1441 ttgacatcgg ccgctgctcc ggctccctcg aaaacagccc cgaccctcgc gtcactgagg
     1501 agctcccggg cgaagaaagc cgtgatatac cgaaccgaga aggcgaggac ggtggacggg
     1561 ttcgcagttc ccttcgtgca caagctgcag aagtggcagt tggcagggtc ttgaaacatg
     1621 gtgtggtcgg ccttgagcgc cgtgatggcc acggcgggag aagccgctac atcgtaaagt
     1681 gcttgatagt tgaacccagc aggagcgcag gagagcttgt tgaccgtctc accgataaac
     1741 gcgacgggaa tgccgatatt tgcgagctgt gcgcccgctt tggcggtgcc gaacgataaa
     1801 tcgaccggat caagcccgaa cactcctttg acgctgcccg gcgagccctc tgcggccagc
     1861 aacgcgattt gcgcgccccg ggaatgaccc gcgagcccta tccgtgacac gtcgaccatg
     1921 ccatggaggg ggccatcagc gttgatcgca ggcagccacg tcgagacttg gtgggagacg
     1981 tttgaaacgg cgttcaccgc catctctgtg atcgtcggat tgtcattcgg ctcatcggga
     2041 aactttgcct gcgcgggggc gtaagcgagc gttgcatcat cttggagaaa cgcgatgatg
     2101 ccccaactgg caagacgctt ggcgtagggc tcataagcga tgccgctttg cataaaccct
     2161 gacgaaaaga tgacaaccgg atgcggccgt gagtcctttg ggatgtacgc cgtggtcgta
     2221 aaagccccgt tcggtgacgg cacggtgagc acggccgttg tgacagcata gggcccatcg
     2281 gcattgtagc gccctgcggc ttccgcgccg ccggcgaaga ccagaatgat caaaacaaag
     2341 agaccaaaca tgtctaaact tccttgtccg tccatgacca caagttgtgc gatacggttc
     2401 gttatcacag attctcagac ctcggcttca gggcgcgtca acgaggaccg cgtcgggtcc
     2461 gtccatgacc tggcatgggt aatcgatggc gccaccgacg tcgtcgagtg tccattgaca
     2521 agcgctgcca ccgatgccga ttggatcgct ggacgtctcg aggccccccc tgagggcact
     2581 tgctgttacg cccgatctgt cgcaattgcc agacctgacc gctgcgcaat tagtcaccga
     2641 gttcactcgc gaggcgagac ggacaccaat cgacaagacg aagcatccat cggcgtccgc
     2701 cgtcgtcgtg cggacctgcg ctgtcggcat cgattatgtg tctgttggag actgtactct
     2761 actggttgaa ggcacgatcg gtttcgtccg cgttggcgtc gacgatgccg gcgatcctca
     2821 gctcgcgcaa gccttggcag cccttcacgc caaacatagc ggtctcgacg ctaagaccgc
     2881 acaagcgcac atctggccat caatcaagcc cgggcagcta tgaacgaact ctacggcgtg
     2941 ttttcaatca cgccaacgcc ccgctatttc gtgcaagcgg gccagattgc gacgccggcc
     3001 gccagcgtgc atgccctgtt ggcgagcgat ggcctcatgc gtctcgtcga cgtgttcggg
     3061 ctttacacgg ccgacgaact cttcgcggcc gcgcgtaatg agg
//
LOCUS       DATMGX010001035         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_141311, whole genome shotgun sequence.
ACCESSION   DATMGX010001035 DATMGX010000000
VERSION     DATMGX010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141311"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..1380
                     /locus_tag="VNR65_17010"
     CDS             400..1380
                     /locus_tag="VNR65_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20419.1"
                     /translation="MAKSPTTVAGAYPLSDLLFSPRAAFIWNHTRKSVVWMNPAARAA
                     FDLRLEDFSASLSASLIRRFAQFTARKEGGGAKVKIPGVPGLNCSIEILKLADGQDGL
                     IVAELGEGEEIQPAPVISTFRKPAKSPKKTLRETVTGQRRTAPTLTAEELRAFKAVGR
                     KVLRLCEDKDQRSKVAAVAVSLPAPSDIASSPERPAHALRDILAAFDLVLFLGENLDI
                     VGIQGQAVRLGLRKAKLAGKSLGDLVLPYDRTILRRMMKKLRCQAAYGSRDALLISGE
                     NGNAKPCRAILGRWEDGDAAFFLALLSLDLPARLKRPQRLDSLAAARLAA"
     gene            1509..>2019
                     /locus_tag="VNR65_17015"
     CDS             1509..>2019
                     /locus_tag="VNR65_17015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWJ20420.1"
                     /translation="MAHDLVLQTSGLTKEFGGFFAVNGVDLAVRRGTIHALIGPNGAG
                     KTTCFNLLTKFLTPSSGTILYNGEDITRTSPADVARKGLVRSFQISAVFPHLTALENV
                     RIALQRRRGGNFDFWRSQSVLNAMNEKACEMLDAVGLSEFAETPAAELSYGRKRALEI
                     ATTLALDPEV"
BASE COUNT          427 a          596 c          578 g          418 t
ORIGIN      
        1 cggacgactt caagacaacg tcatgatcct ggcgagcagc ctcgataaaa cggttgtaag
       61 cttcgcgggc ttggtcgacg ctcttggctg cgaggtcacg cacggttgcg ggaatttcga
      121 aatccattgg cttgttcata gcggtctcct atttacagtc atcctcgctg aggcgcgcgc
      181 aggcctcagg ctcgtctaac tatataaggc agaaattggt gcagcgcaac acgaaacctg
      241 ttgcaccgca ccaaatctta tcttctctgt cagtgccggt cgcatttcca caaaaattac
      301 tgcccgtgtg agcttgcggg gccttgcatt tgcaggcgaa cacgctcttg ttacaagaga
      361 gtgagtcggg gcgatcggag ggaacgcggg aatcaaacat tggcaaaaag cccaacaacc
      421 gtcgccggtg catatcctct ttcggatctc ctcttctcgc cgcgcgccgc cttcatatgg
      481 aaccacacac gcaagagtgt cgtctggatg aaccccgcgg caagagcggc gtttgatctc
      541 cgtttggaag atttttccgc ctcgctttcg gcaagcctca tccgccggtt cgcgcagttt
      601 acagccagga aagaaggcgg cggcgccaag gtaaaaatac ccggcgtgcc tggccttaac
      661 tgctccatcg agatcctgaa actcgccgac gggcaagatg gtctgatcgt cgccgaattg
      721 ggcgagggtg aggaaatcca gccggcgccg gtcatttcca cattccgcaa gcctgcgaaa
      781 agccccaaga agacccttcg agagacagtg acagggcagc gccgaacagc gcccacgctt
      841 acagcggaag aattgcgcgc gttcaaggct gtggggcgta aagtcttgcg gctgtgcgag
      901 gacaaggacc agcgctccaa agtggctgcg gtggcggtgt ccctgcccgc gccttcagac
      961 attgcctcgt cgcctgagcg gccggcgcat gcgctgaggg atattctcgc agccttcgac
     1021 ctcgtactct tccttggcga aaatcttgat atcgtaggaa ttcagggcca agcggtgcgg
     1081 ctcggtttgc gcaaagcgaa acttgccggg aaatcccttg gcgaccttgt cctgccttat
     1141 gatcgcacga tcctccggcg gatgatgaag aagctccggt gtcaggctgc atacggttcc
     1201 agagatgccc tccttatcag cggcgagaac ggaaatgcga agccctgccg tgccattctc
     1261 ggacgttggg aagacggcga tgcagcgttc ttcctcgccc ttctttcact agacctgcct
     1321 gccaggctga aacgcccgca gcgcctggat tcgctcgcgg cggcgcgcct cgcagcttag
     1381 atgagtggag cgaattgaca tttgagtgct gaaatcgaac cactagaata agcatacaat
     1441 ttatctactt ttgtctgggc tgacatttca gcgggcgccg cgcaatgttc ggctgaagga
     1501 acgaagcgat ggcacacgac ctcgttttgc agacatccgg gctcacaaaa gagttcggag
     1561 ggttttttgc cgtgaatggc gtcgacctcg ctgtccggcg cggaactatc catgcgctga
     1621 tcggcccaaa cggcgcggga aagacaacct gcttcaatct cctgacgaag ttcctcaccc
     1681 cctcaagcgg gacaatcctc tataacggag aggatattac gcgaacgtcc ccggcggacg
     1741 tggcccgcaa agggcttgtt cgctcgttcc agatttcagc ggtgtttccg catctgaccg
     1801 cgctcgaaaa cgtccgcatc gcgctgcagc gacgccgcgg cggcaatttc gacttctggc
     1861 gctcccagtc cgttctgaac gccatgaatg aaaaggcctg cgaaatgctc gacgccgtgg
     1921 gcttaagcga gttcgcggaa acgcccgcag ccgagctttc ctatggccga aagcgcgccc
     1981 tcgaaattgc gaccacgctg gcgctcgatc ccgaggtca
//
LOCUS       DATMGX010001036         2394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_409342, whole genome shotgun sequence.
ACCESSION   DATMGX010001036 DATMGX010000000
VERSION     DATMGX010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409342"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..360)
                     /locus_tag="VNR65_17020"
     CDS             complement(67..360)
                     /locus_tag="VNR65_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20421.1"
                     /translation="MNAEPRKTWTVAEAKARLSEILRLASEEGPQRIGTKTPYVVVPE
                     EAWLKRAEPRMPLGRWLVENMPRGYDLELPDRHEPDRLSPFDDWEFEDGKDAK"
     gene            520..>2394
                     /gene="mutS"
                     /locus_tag="VNR65_17025"
     CDS             520..>2394
                     /gene="mutS"
                     /locus_tag="VNR65_17025"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWJ20422.1"
                     /translation="MSSIKQKTEARENAGDVKSATPMMAQYLEVKAKHPEFLLFYRMG
                     DFFELFFDDAVAAANALGIQLTKRGKHSGEDIPMCGVPVARADDYLQKLIRKGFRVAV
                     AEQLEDPELAKKRGPKAVVRRDVVRLVTPGTLTEDALLVSVSNNFLAALARNGKGAAA
                     RYTIAALDISTGEFLLSDAAAPDLAGELLRLRPAELLIPDDELADEAAKAASEAAGAS
                     LSPLPRGYFAKQRGERALKEAFEVGALDGLGDFEDGDLAVIGALLHYVNLTQMGERPA
                     LQPPRREAMAALLSIDAATRASLELVRPRNEGAPTVFSAIDRTVTAAGARELMSRLVS
                     PSTDVELINGRLDAVAAFIEEWALRECVRTILKTAPDMSRALSRLKLKRGGPRDLGAI
                     RDGLAIAKDLSALLAGSPAQPAHLADILSALSPFSLADGRAEASLARTLDRALGDALP
                     FFSRDGGFIASGYDAALDELRELQSSTKVVLAKLQADYAAKTGIKTLKIQYNQVFGYF
                     VEVGPASASALQAEPHARLFRHKQTLANAVRFTTDELSALESRILNATSDALGRELAL
                     FNQLAAAVLDAETAIARAASALAALDCLAALAELAQAQNYVRPRVDNSRCFLIEGGRH
                     P"
BASE COUNT          420 a          763 c          761 g          450 t
ORIGIN      
        1 caagaacttt cgcgttcggc tttcccttca ccagttccga gatgatgttc gtgtcgagga
       61 ggacgctcat tttgcgtctt taccatcttc gaattcccag tcgtcgaagg gtgatagccg
      121 gtcaggctcg tggcggtccg gcagttcaag gtcatagcct cgtggcatat tttcgacgag
      181 ccaacgcccg agaggcatgc gcggttcggc gcgcttcagc catgcttcct cgggcacgac
      241 cacatagggc gtctttgtcc cgatgcgctg cgggccttcc tcgctcgcaa ggcgaaggat
      301 ctccgaaagc cttgccttgg cctccgccac tgtccaggtt ttccgtggtt cggcgttcat
      361 gaccctctcc ctactgatag tccaatctag tctagtctaa tttggtgtca aggcgcgtca
      421 gagtagtcaa gcggggataa agcgccgccc gcacttctcg ctgcaacaga tgcgcgtaaa
      481 cttccccgct tgcgggtacg agtcgggtgg agaatggact tgtcgtcgat caagcagaag
      541 accgaggcgc gcgagaacgc gggcgacgtg aagagcgcca cgcccatgat ggcgcaatac
      601 cttgaggtaa aggcgaagca cccggaattt ctcctcttct accgcatggg cgatttcttc
      661 gagctgtttt tcgacgacgc ggtggcggcc gcgaatgcgc tcggcatcca gctcaccaag
      721 cgcggcaagc attccggcga agacatcccc atgtgcggcg tgccagtcgc gcgcgccgac
      781 gattatctgc aaaagctcat ccgcaagggc tttcgcgtgg ccgtggccga gcagcttgaa
      841 gacccggagc ttgcgaagaa gcgagggccg aaagccgtcg ttcgccgcga tgtggtgcgg
      901 ctcgtaacac ccggcacgct caccgaggac gcgcttctcg tttccgtcag caacaacttc
      961 cttgctgcgc tcgcgcgaaa tggcaagggt gcagcggcgc gctatactat cgctgcgctc
     1021 gatatctcca ccggcgagtt ccttctcagt gacgcggcag cacccgatct ggcgggcgag
     1081 cttcttcgcc tgcgcccggc cgaactgctg atccccgacg acgaactggc agacgaagcc
     1141 gccaaggcgg cgagcgaagc cgcgggcgct tcgctgtctc cccttccgcg cggatatttc
     1201 gcgaagcaga ggggcgagcg cgccctgaaa gaagccttcg aggtgggagc gctcgacggg
     1261 ctgggcgatt tcgaagacgg cgatctcgcc gtgatcgggg cgcttctcca ttatgtgaac
     1321 ctcactcaga tgggcgagcg gcccgcgctt caaccgccga gacgcgaggc aatggctgcg
     1381 cttctgtcca tcgacgcagc tacacgcgcg agcctcgaac tggtgcggcc gagaaatgag
     1441 ggcgcgccca ccgtcttctc cgccatcgac cgcacggtca cggcggcggg cgcgcgcgaa
     1501 ctcatgagcc gcctcgtgtc gccctcgaca gacgtggagc tgatcaacgg gcggctcgat
     1561 gcagtcgcag ccttcatcga ggaatgggcg cttcgggaat gcgtccgcac catcctgaaa
     1621 acggcgccgg atatgagccg cgcgctgtca cggctgaaac tgaagcgggg aggtccgcgc
     1681 gatctgggcg cgatccggga tggccttgcc attgcgaagg atttgagcgc gcttctggcg
     1741 ggatccccgg cgcagcctgc gcatcttgcc gatattctga gcgcgctttc gccgttctca
     1801 ttggcggacg ggcgagccga ggccagtctt gcacgcacac tcgaccgcgc gctcggggat
     1861 gcgcttccgt tcttttcgcg cgacggtggc tttatcgcga gcggctacga tgcggcgctc
     1921 gacgagcttc gcgagcttca gtcgagcacg aaggtcgttc tggcaaagct gcaggccgac
     1981 tatgccgcga aaacgggcat caagaccctg aaaattcaat ataatcaggt cttcggctat
     2041 ttcgtggaag tcggccccgc atcggcgtcg gcgttgcaag ccgagccaca cgcgcgcctc
     2101 ttccggcata agcagacgct cgccaacgcg gtgcgcttca cgaccgatga gctgtcggcg
     2161 ctggaaagcc gcatcctgaa cgcgacgagc gacgcccttg gccgcgagct ggcgctgttc
     2221 aatcagctgg ccgccgccgt tctcgatgcg gagaccgcga tcgcgcgcgc ggcgtcggcg
     2281 cttgccgcgc tcgactgcct ggccgcgctc gcggaattgg cgcaggcgca gaattatgtg
     2341 cggccgaggg tggacaacag ccgctgcttc ctcatcgaag gcggccgcca tccc
//
LOCUS       DATMGX010001037         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_459296, whole genome shotgun sequence.
ACCESSION   DATMGX010001037 DATMGX010000000
VERSION     DATMGX010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459296"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1083
                     /locus_tag="VNR65_17030"
     CDS             199..1083
                     /locus_tag="VNR65_17030"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HWJ20423.1"
                     /translation="MKINAKDFLVRPKEEVNLSTCPTRVKAFCKSKKRYRALLKEHVE
                     GLSSLQRLHYASNRYALLLIFQGMDAAGKDGAIRHVMSGVNPQGCEVFGFKQPSTEEL
                     EHDFLWRTIRHLPERGRIGIFNRSYYEEVLIVRVHPEILRSQGLAAELLDEKTIWQDR
                     YRSIIDLEEHLHRNGTRVVKVFLHLSKDEQRKRFLERIDEPAKNWKFSLSDIHERTYW
                     NQYMRAYEACLSATSTHQAPWYVVPADDKENARLLVSQIVLDALNGLDMAYPKTSAKR
                     RRELQSIRTLLAKRSRKQ"
     gene            complement(1399..1638)
                     /locus_tag="VNR65_17035"
     CDS             complement(1399..1638)
                     /locus_tag="VNR65_17035"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWJ20424.1"
                     /translation="MYWYQWNFFGMHFFWWMFWIVLIIIVFGWATPVPRRRVRLYRED
                     ALSILQRRYAAGEITTAEYDERKARIQKEAGHPRP"
     gene            complement(1696..>2047)
                     /locus_tag="VNR65_17040"
     CDS             complement(1696..>2047)
                     /locus_tag="VNR65_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20425.1"
                     /translation="PPLRSSREVTGYHIHATDGDIGHVEDFLVDDVSWAIRYMVVDTI
                     NWWPGKKVLVAPEWIERVDWDKSMVHVHLGCEKIRTSPEYDPARPIERAYETRLYGHY
                     AQPRYWDEWHDKGI"
BASE COUNT          429 a          606 c          639 g          373 t
ORIGIN      
        1 gaagcctgat gccgcggcca cggcagcgcc cgatgtgacg ccgcggaccg tcacccgagt
       61 ccgtcaactc tacgagcagc tggggcgcga ggatgttcgt tcagttgagg agtgggagaa
      121 agccgagcgc gaaaagcgga acgataaacc caagaagtag agagaagcga cgcagcagat
      181 gcaaccaggg agacgacgat gaagatcaac gcaaaggatt tccttgtacg gccaaaggaa
      241 gaggtcaacc tcagcacgtg tccgacgagg gtaaaggcgt tttgcaagtc gaaaaaacgg
      301 tatcgcgcgc tcctgaaaga acacgtcgag gggttgagct cgctgcagcg ccttcactac
      361 gcgtccaacc gctatgcgct gctgctgatc ttccagggga tggacgccgc cggcaaggat
      421 ggcgccatcc ggcacgtcat gtccggggtc aatccgcagg gctgcgaggt gttcggtttc
      481 aagcagccga gcaccgagga gctggaacac gattttctct ggcggacgat ccgccatctt
      541 ccagaacgcg ggcggatcgg catcttcaac cgttcctact acgaggaggt gctcatcgtt
      601 cgggtccacc cggagatcct ccgcagccaa gggcttgcgg ccgagttgct cgacgagaag
      661 accatttggc aggatcggta tcgttccatc atcgacctgg aagagcatct ccaccgcaac
      721 ggcacccggg tcgtcaaggt ctttctccac ctgtcgaagg acgaacagcg aaagcggttc
      781 ctcgagcgca tcgacgagcc ggccaagaac tggaagttca gcctctcgga cattcacgag
      841 agaacatact ggaatcaata catgagggcg tatgaggcgt gcctgagcgc gacaagcacc
      901 catcaggcgc catggtacgt cgttcccgcc gatgacaagg agaacgcccg gctgctcgtt
      961 tcccagattg tcctcgatgc gctcaacggg ctcgacatgg cctatcccaa gaccagcgcg
     1021 aaacgccgga gggaactgca atccatacgc acgctgctcg cgaagaggag ccgcaagcaa
     1081 taaagaaccg tcgcggctta tgtcgggtgc atgttcgggc tgaaggtgcc gagcacggcg
     1141 cggccgcagg tctgatacca atttgccatc tgtcgtagag gactgcctgt cactgcgagg
     1201 agcgaagcga cgaagcagtc tcgtggtcca gggagggtcg tggacaccgg tgtagtgacc
     1261 ccctgaggtc aagccactag gttgagttgt tgggcacgat actcggcggg gctcttgtag
     1321 ccgagggctg agtgcgtcct tgcctttgtc cgtcttgagg aaccgtgcat cctgaccgag
     1381 gtcggtttgc gaggacggtc agggcctggg atgacccgct tccttctgga tgcgagcctt
     1441 gcgctcgtcg tattctgctg tcgtgatctc accggccgcg tagcgacgtt ggaggatgct
     1501 aagcgcgtcc tcgcggtaca gccgcaccct cctgcgcggg acaggggtcg cccagccgaa
     1561 gacgatgatg atgagcacga tccagaacat ccaccagaag aaatgcatcc cgaagaagtt
     1621 ccattgatac cagtacatga tgtcctcctc tgcccgtgat gcgcatgatc agtgggcggc
     1681 cgcgaactgt aacgctcaga tgcccttgtc gtgccattcg tcccagtaac gtggctgggc
     1741 atagtggccg tacaggcgcg tctcgtaggc gcgctcgatg ggccgcgccg gatcgtactc
     1801 agggctggtc ctgatcttct cgcaccccag gtgcacgtgc accatcgact tgtcccaatc
     1861 cacgcgctcg atccattcgg gcgccaccaa caccttcttg ccaggccacc agttgatggt
     1921 gtccacgacc atgtagcgga tcgcccagga aacgtcgtcc acgagaaagt cctccacgtg
     1981 gccgatgtcg ccgtccgtcg cgtggatgtg gtagccggtc acctcgcgcg agctgcgcag
     2041 gggcggg
//
LOCUS       DATMGX010001038         2895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_143392, whole genome shotgun sequence.
ACCESSION   DATMGX010001038 DATMGX010000000
VERSION     DATMGX010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143392"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            454..1368
                     /locus_tag="VNR65_17045"
     CDS             454..1368
                     /locus_tag="VNR65_17045"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HWJ20426.1"
                     /translation="MVSHKVIKCFRVTRHLSIPELGLPVWTFLVLAIGVAIAAFLPIS
                     HATAKVGWTSPHFQDHPLAGTIWTSDFEPVTISELEKALANARFVLLEEIHNNPDHHR
                     LQARLIDALVSKGRRPAIVFEMIPTNLQVELDRHVQGGVREASTLGKALRWEERGWPD
                     WAIYQPIAEAALSAGLPLKAGGLGADVQKTFAKGELPPTINELDLAPEVAEAEAREIK
                     EGHCNLLPADAVEPMITMQRVLDASLAKATVSANAADGAVLIAGAGHVRNDWALFRIS
                     SVRNCPMPPSFRLPSSRSTRSAPRRQST"
     gene            1365..1499
                     /locus_tag="VNR65_17050"
     CDS             1365..1499
                     /locus_tag="VNR65_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20427.1"
                     /translation="MKTVSGLEKPFDFIYLTPSADLTDHCAEIEKHLKTEKAEKTGER
                     "
     gene            1628..2341
                     /locus_tag="VNR65_17055"
     CDS             1628..2341
                     /locus_tag="VNR65_17055"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HWJ20428.1"
                     /translation="MDKSSELPADDPTLAVSAKIRNRLRLAKQRFHANDNISPFIEPG
                     ELDRLLEEVTVKMKGVLESLVIDIESDHNTQDSARRVAKMYLEEVFRGRYFPPPPVTE
                     FPNIANLSELMIVGPVTVRSACSHHLCPIMGRLWIGLMPNQHSNLIGLSKYARLAEWI
                     MTRPQIQEEAIIQMADLLMGVVKPDGLAIVLEADHFCMHWRGVKDTASKMTNSVMRGS
                     FLMDPALRREFLSLVTLNS"
     gene            2352..2783
                     /locus_tag="VNR65_17060"
     CDS             2352..2783
                     /locus_tag="VNR65_17060"
                     /inference="COORDINATES: protein motif:HMM:NF016807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HWJ20429.1"
                     /translation="MLVRCLYASRAAKPLASVVIDQILEQSQKGNPRRGITGLLCFTG
                     DIFVQVLEGGRDEVCELFNAIVRDDRHQDVRLLVYEEIAERNFGNWTMGQVKIESINP
                     ALLLKYYEKAELNPFSCSGKATMALLQELLAAGVISHRGTS"
BASE COUNT          636 a          776 c          829 g          654 t
ORIGIN      
        1 agcatttttt ggcgaagtag cttcggggca gcggcttttt gcgttcaagg cgttcgagtc
       61 cggatcggaa tggaagaacc gccgctcatc cttcagctga aaacgtctca atttggcccc
      121 aggcagaagg tcggcgtggc atggcctacg ttagttcgag aacggcccga gacgcgtgat
      181 ttcacgtcag cacgtggcta ttacgggccg aaacttcggc tcacggcctg ttgcgttggt
      241 tttggggtgc ctcaggcgtg cataagaaag tatctacttc gatttgcccg aaaggagtcg
      301 aaacgagaaa gtctcagagt ttgcagacgt gtgctgctgc tccttcgaaa ccggacttgc
      361 gattggcacc ctgacgtagc tgattaagag ccttgagggg cagagttgca gacatcatct
      421 cactcaggag cagcgagcgc ggcgcaggga tcgatggtca gtcacaaagt tataaagtgc
      481 tttagggtca cgcgtcacct atcgatccca gagcttggat tgccggtctg gacctttctt
      541 gttctggcca taggcgttgc catcgcggcc tttctcccga taagtcatgc gaccgcgaag
      601 gtcggctgga cttcgccaca tttccaagac catcccctcg ccggcacgat ctggacctcg
      661 gattttgaac cggtcacgat cagcgagctt gagaaagctt tggcgaacgc gcgtttcgtg
      721 ttgctcgaag agattcacaa caatcccgat catcatcggt tacaggctcg gctcatcgat
      781 gcgcttgtca gcaagggccg gcgtccggct attgtctttg agatgatccc cacaaatctg
      841 caagtggagc tcgatcgtca cgtccaaggt ggcgtgcgcg aggcaagcac gctcggcaag
      901 gcattacgct gggaagagcg tggctggccc gattgggcca tctatcaacc cattgcggag
      961 gcagcgctct cggccgggtt gccgctgaag gccgggggac ttggcgcaga tgtgcaaaag
     1021 acgttcgcca agggggagct gccgccaacc ataaatgaac tcgacctggc accggaagtc
     1081 gctgaggcgg aagcccgtga gataaaagag gggcattgca atcttctgcc cgccgatgcg
     1141 gtggagccca tgattacaat gcagcgcgtt ctggatgcct cccttgcaaa ggcaacggta
     1201 tcagcgaacg ctgctgacgg agcggtgttg attgctggcg cgggacatgt acgaaacgac
     1261 tgggctctgt ttcgcatatc atccgtcaga aattgcccaa tgccaccctc gtttcgattg
     1321 ccttcgtcga ggtcgacccg cagcgcacca cgccgtcaga gtacatgaag acagtttctg
     1381 gtttggagaa accgtttgac ttcatctatc tgacaccgag cgcagacctg actgaccatt
     1441 gcgccgagat cgagaaacat ctgaaaaccg agaaggctga gaagactggc gaacgctaac
     1501 cgtcgacggc gtctgactgg tagccggcta tctggagcgc agccacatgc ggcttgggtt
     1561 ggcttctggc atattaggtc tatcgtgagt ggttcgaccc tgattttaga ttgaaagagg
     1621 gcttcgcatg gacaagtctt cagagcttcc tgccgatgat ccgaccctcg cagtctctgc
     1681 gaaaatccgt aatcgcctac gtctggcaaa gcaacgcttt cacgccaacg acaacatttc
     1741 tcccttcatc gagccgggag agctcgaccg gctgctcgaa gaagtcaccg taaagatgaa
     1801 aggcgtgctg gaaagtttgg tcatcgatat tgagagcgat cacaatacac aagactctgc
     1861 gcgccgtgtc gccaagatgt atctggagga agtgtttcga gggcgatatt ttccgcctcc
     1921 gcccgtgacc gaattcccga acattgcgaa cctgagcgaa ttaatgatcg tcggccctgt
     1981 tacggtgcga agcgcgtgca gtcaccatct ttgtcccatc atgggccggc tctggatcgg
     2041 cttgatgccc aaccagcact cgaatctgat cggattgtcg aaatatgcgc gacttgccga
     2101 atggatcatg acccgcccgc aaattcagga agaagccatc attcagatgg ctgaccttct
     2161 gatgggggtg gtcaagcccg atgggctggc gatcgtcctc gaggccgatc acttctgtat
     2221 gcattggcgc ggcgttaaag atacggcgtc aaaaatgacg aatagcgtca tgcgcggctc
     2281 ttttctcatg gaccccgcct tgcgccgcga atttctttcg cttgtcactc tgaatagctg
     2341 atgaggttgc gatgctcgtc cgttgtctct acgccagtcg cgccgcgaag ccgctcgctt
     2401 cggtcgtgat cgatcaaatc ctggagcaat cccaaaaagg caatccgcgc agagggatca
     2461 cgggcctcct ctgtttcacc ggcgatattt ttgttcaggt cctcgaaggc ggccgcgatg
     2521 aagtctgcga attatttaac gccatcgttc gggatgatcg gcatcaggat gtgagactcc
     2581 tggtttacga agaaatcgct gaaagaaatt ttgggaactg gacgatgggg caggtcaaga
     2641 tcgagagcat caatcctgcg ttattgctaa aatactacga gaaagccgaa ctcaatccat
     2701 ttagctgttc tggcaaagct accatggcct tgctgcagga actcttggcg gcgggggtca
     2761 tttcacaccg cggcacttct tagttacagc cctgtccggg ttcatggaac gccagacagg
     2821 ttgccacgcg tgcccgattg taaaacggga ttagcttttt tccgcagaac taggcacggc
     2881 agcgcttgcg gcgct
//
LOCUS       DATMGX010001039         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_267119, whole genome shotgun sequence.
ACCESSION   DATMGX010001039 DATMGX010000000
VERSION     DATMGX010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267119"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..1408
                     /gene="hflK"
                     /locus_tag="VNR65_17065"
     CDS             350..1408
                     /gene="hflK"
                     /locus_tag="VNR65_17065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HWJ20430.1"
                     /translation="MSSQNGNGGRGGPWGPRAEPDSDSEGLVRQGQDRLRQITPRGSP
                     RGAIAVAALALFGFGVWTAYYTVPSDSVAVVQRFGEYLKDVPPGLHFKLPLGVDVATI
                     VPVKRQLKQEFGFGTQGATDPAQASRDAKRETQMVTGDLNAALVEWVVQYRISDPVKF
                     LFDVREPKGTLRDVSESVMREVVGDRTVDEVITIGRQEIEAEALTKMQALSTKYAMGI
                     SIDQVQLKDINPPVPVQASFNEVNQAQQEKEKLINEARRDYNKVIPLAEGEKDQRIRE
                     ADGYRLKRINEAEGDVARFSALLTEYIKAPVVTRRRIYLETLQDVLPGIRSKIIVDEQ
                     TRSILPLLNLDPQKGERP"
     gene            1405..>2037
                     /locus_tag="VNR65_17070"
     CDS             1405..>2037
                     /locus_tag="VNR65_17070"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HWJ20431.1"
                     /translation="MTRIISLTVLVFFGIAMFVLASSLYTVSEVEQAIITQFGKPVGT
                     PVTTAGLKIKVPFIQDVNLIDKRVLEWDGSPSDMPTKDKLYVSVDLFARWRIVDPLQY
                     FLRLHDERSAQSRLDDVLGSETRNAVAKHELIEIIRTTKDRVPLRDNLLTDAGRELDV
                     GSLVPIQKGRKLVEGEILAEAAEKVRVFGMELLDIRFKRINYNESVRPKIY"
BASE COUNT          457 a          579 c          612 g          389 t
ORIGIN      
        1 cgcaagcagc caagctccaa tggtgacgcg ttcgaaccaa aaccgtcgtc ttcaaccatc
       61 ccttcgagct gaaaggtgtc gatcgagtat taccgcctgg aaactatcga cttgtgaccg
      121 acgaagaatc atccaggaac ggtctttcca gttaccgcga tctcgaccat gatatttgtg
      181 ccggggcaca cttgtaatgc ttcgatcgag cccggtgagc caagacgaag ggatgcgtcc
      241 aggtaaccca accgctccgc cgcgcgagca aatgactgct tcatgcagcg ttaggccggt
      301 cagcgcgatg cgaagacact gagctcaagc gcaagatgag gataatcaaa tgtcatcgca
      361 gaacggcaac ggtggccggg gcggcccctg gggccctcgg gccgaacccg attccgatag
      421 tgagggtctg gtccggcagg ggcaggatcg actcaggcag atcacgccga ggggtagccc
      481 gcgcggagca atcgccgttg ccgccctcgc tctgttcggc ttcggcgtgt ggacggccta
      541 ctacactgtg ccgagcgact ccgtcgctgt cgttcaacgc ttcggtgagt atctcaagga
      601 cgttccgccg ggcttgcatt tcaagctgcc gcttggcgtc gacgtcgcga caatcgttcc
      661 cgtaaaacgg cagttgaaac aggagttcgg gttcggcacc caaggcgcca ccgatccggc
      721 tcaggcttct cgcgacgcaa agcgggaaac gcaaatggtg acgggtgacc tgaacgccgc
      781 attggtcgaa tgggtcgttc aataccgcat ttcggacccg gtgaagttcc ttttcgacgt
      841 tcgtgaaccg aagggaacgc tgcgcgatgt gtccgagtcc gtcatgcggg aagtcgtcgg
      901 cgatcgcacc gtggacgagg tgatcaccat cgggcggcag gagatcgaag cggaagcgct
      961 gaccaagatg caggcgctct cgaccaaata cgcaatgggg atcagcatcg atcaagtgca
     1021 attgaaggac atcaatccgc ccgtgccggt gcaggcgtcg ttcaacgagg tcaaccaggc
     1081 tcaacaggag aaggagaagc tgatcaacga agcccggcgc gattacaaca aggtgatccc
     1141 gttggccgag ggcgagaagg atcagaggat ccgcgaagcc gatggctacc ggctcaaaag
     1201 gatcaatgaa gcggaaggtg atgttgcccg gttcagcgcc ttattgacgg aatatatcaa
     1261 ggctccagtg gtgacccggc gccggatcta cctcgaaacc ctgcaagacg tcctgccggg
     1321 catacgctcc aagatcattg tcgatgaaca gacgcgcagc attctgccat tgctgaatct
     1381 cgacccccag aagggagaac ggccatgacg agaattatct cactaactgt tctggtgttt
     1441 tttggcatcg ccatgttcgt tttggcgagt tcgctttata ccgtaagcga agttgagcaa
     1501 gccatcatca cccagttcgg caagcccgtc ggcacccctg tgaccaccgc cggtctcaag
     1561 atcaaggtgc ccttcatcca ggacgtcaac ctgatcgaca agcgtgttct cgagtgggat
     1621 ggcagtccgt ccgatatgcc aaccaaggac aagctctatg tctcggtcga cctcttcgcc
     1681 cgctggcgga ttgtcgatcc tttgcagtac tttctacggc tgcacgatga gcgcagcgct
     1741 cagtcccgcc tggacgacgt tcttggcagc gagacgcgga acgccgtcgc caagcatgag
     1801 ctgatcgaga tcatccgcac aaccaaggat cgcgtgccgc tgcgcgacaa tctcctgacc
     1861 gacgcgggcc gggagctgga tgtggggtca ctggtgccga tccagaaggg acgcaaactg
     1921 gtcgaggggg agatccttgc ggaggccgcc gagaaggttc gagtgtttgg aatggaattg
     1981 ctcgatatcc ggttcaagcg gatcaattac aatgaaagcg tgcgcccgaa gatctac
//
LOCUS       DATMGX010001040         3304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_198151, whole genome shotgun sequence.
ACCESSION   DATMGX010001040 DATMGX010000000
VERSION     DATMGX010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198151"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /gene="bchE"
                     /locus_tag="VNR65_17075"
     CDS             complement(<1..1425)
                     /gene="bchE"
                     /locus_tag="VNR65_17075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     anaerobic oxidative cyclase"
                     /protein_id="HWJ20432.1"
                     /translation="MRIILIHPNYHSGGAEIAGNWPPAWVAYICGPLKAAGYTDIRFV
                     DAMTNNLSEDDIRAIIEKEKPDIVGATSITPSIYKAERLLQIAKEAHPGTVTFLGGIH
                     ATFMYQQVLTEAPWIDAIVRGEGEAIFVNAVRSVEKGTWLQDRAEIKGIAYTDEDKKI
                     VATPSEPPISNLDSISPDWSVLEWEKYIYIPTGTRVAIPNLARGCPFTCSFCSQWKFW
                     RNYRIRDPKKVVDEIETLVRVHKVGFFILADEEPTINRKKFVEFCEELKSRKLPVLWG
                     INTRVTDILRDEALLPLYRAAGLIHISLGTEAAAQLKLDRFNKETTIAQNKRAIELLR
                     KNGIVSEAQFIVGLENETPETLEETFRMAMDWKPDMANWAMYTPWPFSDLFKELSDKV
                     EIFDYEKYNFVTPIMKPDAMERTELLNHVMRNYRRFYMRKAFLGYPWVWSGQRRSYLL
                     GCLKAFLKSGWERKFYDLGRVKYWG"
     gene            complement(1587..2054)
                     /locus_tag="VNR65_17080"
     CDS             complement(1587..2054)
                     /locus_tag="VNR65_17080"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWJ20433.1"
                     /translation="MIKGLANTVRAALAALVIFSFFIMPSAFGDVLFKPASLKIETKT
                     GPVLLNVEVADTEAKREQGLMFRRSISAGHGMIFLFDGEHEITMWMKNTYIPLDMVFI
                     GSDWRIRHIAYDAEPFSTDVISSMRPASRVLEIGAGQAKKLGLAVGDSVALQK"
     gene            complement(2068..2679)
                     /locus_tag="VNR65_17085"
     CDS             complement(2068..2679)
                     /locus_tag="VNR65_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWJ20434.1"
                     /translation="MPSFSVEAGDYPMGDKLPPTIASFNDIAEQPSIDVFVIAGHIKW
                     FDVSKGFGFIVPDGGLPDVLIHVTVLRRDGYQTAYEGARIVCEVLRRDRGLQAFRVIE
                     MDESTAIHPSQLPQRTHVHVMPESDWQRAMVKWFNRLKGFGFLTRGAGTEDIFVHMET
                     LRRFGFAELRPGQIVQVRFGHGDKGLMAAELRPDGAVGYPSSH"
     gene            3122..>3304
                     /locus_tag="VNR65_17090"
     CDS             3122..>3304
                     /locus_tag="VNR65_17090"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWJ20435.1"
                     /translation="MKYLHTMVRVTDIDQSLDFYVNKLGLQEVRRTESSAGRFTLIYL
                     AAAGDENAQVELTYNWD"
BASE COUNT          737 a          945 c          881 g          741 t
ORIGIN      
        1 accccaatat ttcacgcgtc ctaggtcgta gaacttccgc tcccagccgc tcttgaggaa
       61 cgccttgaga cagcccagga ggtagctgcg tctctgcccg ctccagaccc acggatatcc
      121 taggaaagcc ttgcgcatgt agaagcgccg gtaattgcgc atgacgtggt tgagcaactc
      181 cgtccgctcc atggcgtccg gcttcatgat cggcgtcacg aagttgtact tctcgtagtc
      241 gaagatttcg accttgtcgc tcagttcctt gaagaggtcc gagaacggcc agggcgtgta
      301 catggcccag ttcgccatat ccggtttcca gtccatcgcc atccggaacg tctcttcgag
      361 cgtctccggg gtctcgttct caaggcctac aatgaattgc gcttcggaga cgatgccgtt
      421 cttgcgaaga agctcgatgg cgcgcttgtt ttgcgcgatt gtcgtctcct tgttgaagcg
      481 gtcgagcttc aattgcgcag cggcttccgt tccaagcgag atgtggatca agcccgcagc
      541 acggtaaagc ggaagcagcg cctcatcccg gagaatgtcg gtaacccgcg tgttgatgcc
      601 ccacaggacc ggtagtttac ggctcttcaa ctcttcgcag aattcgacaa acttcttccg
      661 gttgatggtc ggctcttcgt cggccaggat gaagaagccc accttgtgga cgcggacgag
      721 cgtctcgatt tcatcgacga ccttcttcgg gtcgcgaatg cggtagttcc gccagaattt
      781 ccattgggaa cagaagctac acgtaaaggg gcagccccgg gcaaggttcg ggattgcgac
      841 acgcgtgccg gtcgggatgt aaatatactt ttcccattcg agcacgctcc aatccgggct
      901 gatgctgtcg aggttcgaga ttggcggctc ggaaggcgtc gccacaatct tcttatcctc
      961 gtcggtatag gcgataccct tgatctcggc gcgatcctgc agccaggtgc ccttttcaac
     1021 cgagcgaacg gcgttcacga aaatcgcctc gccctcgcca cgcacaatgg catcgatcca
     1081 gggagcttcg gtaagcacct gctgatacat gaacgtggcg tggatgccgc caagaaacgt
     1141 caccgtgccg gggtgagctt ccttcgcgat ctgcaacagc cgctcagcct tgtagattga
     1201 cggtgttatc gatgtcgcgc cgacgatatc aggcttttct ttctcgataa tggcgcggat
     1261 atcgtcttcc gacagattat tcgtcatcgc gtcgacgaag cgaatgtcgg tgtaacccgc
     1321 ggccttcaga ggcccgcaga tgtatgcaac ccaggcagga ggccaatttc ccgcgatctc
     1381 cgctccgccc gagtgataat tgggatggat caggatgatt cgcatagggc gccccggtaa
     1441 aaatgtcaga tcaagttgac aggagtttgt gtcaagcgga gattactccc ttgttgtgct
     1501 tccgttttga tatagatcaa gagaatctcg ccatttgatc aatctgcggc gtcgactttg
     1561 ggctagactt aatcaaattg cgtccctcat ttctgcagcg cgacggaatc cccgactgca
     1621 agcccaagct tctttgcctg tccggcccct atctccagaa cccgggacgc ggggcgcatg
     1681 gaagaaatga cgtcggtcga gaacggctcc gcatcgtagg cgatatgcct gatgcgccag
     1741 tcgctcccga taaagaccat gtcgagcggg atgtaggtgt tcttcatcca catcgtgatc
     1801 tcatgctccc cgtcgaacag aaagatcatg ccgtggccgg ctgagatcga gcggcgaaac
     1861 atgaggcctt gttcgcgctt tgcctcggta tcggctacct cgacgttcaa aagcacggga
     1921 ccagtcttcg tttcgatttt caggcttgcg ggtttaaaga gcacatctcc aaaagctgac
     1981 ggcattataa aaaagctgaa aatgacaagg gccgcaagag cggccctgac agtatttgcg
     2041 agacccttga tcatgggtct atccctttca gtgcgaggag gggtaaccca ctgcgccgtc
     2101 gggacgaagc tcagcggcca tcaggccttt atcgccgtga ccaaagcgga cctgcacaat
     2161 ttgcccaggc cgaagctcgg caaagccgaa gcgccgaaga gtttccatat ggacgaagat
     2221 atcctctgtg cctgcaccac gagtcaaaaa gccgaagccc ttgagccggt tgaaccattt
     2281 caccatggcc cgttgccagt cgctctcggg catgacatga acgtgggttc gttgaggcaa
     2341 ctgcgaggga tggatagccg tactttcatc catctctatc acgcggaacg cttgaagacc
     2401 gcggtcgcgc cggagcactt cgcatacgat acgcgctccc tcataggccg tctgatagcc
     2461 gtcccgccgc agaacggtga cgtgaatcag aacatcagga agcccgccat ctggcacgat
     2521 gaaaccaaac cccttggaaa catcaaacca tttgatatgg ccagcgataa cgaagacgtc
     2581 gatgcttggt tgctctgcga tatcgttaaa agatgcaatt gtgggaggta atttgtcccc
     2641 cattggatag tcccccgctt ctactgaaaa acttggcatc cgcaaacggc ctcgcccaag
     2701 accctcacga ataccaactc acttgcgaaa cataacatca gtctacgccg tggcgagaga
     2761 aaattaactc tttccccagc gcccttttct ggacgtgcct caaccgcttt tgaagacagg
     2821 cccatcaagt atttgattat ccgagtgaaa ctttgacaga atttaggcac agcgggccgc
     2881 gcatgctctt tcgaaagcct cgaacaatgc gttttgaaca gcgcccaggt tgcgcaattt
     2941 ctttcgattg acccgacaaa cagacagcga atcaacatac ttgggtcgct gaagtgcgaa
     3001 tcaggacctg ccgcgagccg cgctttccac acctgttgaa acacctttcg ccaagacacc
     3061 atctatccgc agtagcgtcg tatttgccat accgttcgcc ggcgcaagtt ggaggcccat
     3121 cgtgaagtat cttcatacaa tggtccgcgt gaccgacatt gaccaatccc tcgacttcta
     3181 tgtgaacaag ctcgggcttc aggaagttcg ccgcacggaa agttccgcgg gacgtttcac
     3241 gctcatctat ctggcggcgg ctggcgatga aaacgcgcaa gtggagctga cttacaactg
     3301 ggat
//
LOCUS       DATMGX010001041         4254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_89641, whole genome shotgun sequence.
ACCESSION   DATMGX010001041 DATMGX010000000
VERSION     DATMGX010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89641"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VNR65_17095"
     CDS             <1..1367
                     /locus_tag="VNR65_17095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ20436.1"
                     /translation="LSLRRENFSLRDLLGGVMSQLFSDAEQKGLNLRSDTSAYIPDQL
                     YGDPLRISQCLLNYLSNAVKFTPQGSVIVHVDLEKRSGAGLLLNFEIEDTGIGVEPEA
                     LTRLFSPFEQVDTSMTRKFGGTGLGLAITRRLAMLMGGEAGGTSAPGKGSRFWFSAMV
                     EPAKASSVSARLKSKSAAKPGETFGDARVLVVEDVELNREILLDMLREDGLKADTAEN
                     GQEAVTKAASNPYDIIFMDMQMPIMDGLTATMTIRRMAAHRETPIIAVTANVFEKSRD
                     ECLAAGMNDFLCKPLDPDDLHAALHQWLSKEPAPVAVESHPKARGEKVEGGDLASLRR
                     CLGEIADIDLDCGLRRIKQPERYVRYLRQYGDKYEGSFSHLRDLLAKGKTADAASFAH
                     SLKGASAQLGVVGVAKQAELLQAAIKNEAEEGSIEALLTQSEERCAVVWASIQKLEVS
                     TPAH"
     gene            complement(1385..2278)
                     /locus_tag="VNR65_17100"
     CDS             complement(1385..2278)
                     /locus_tag="VNR65_17100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ20437.1"
                     /translation="MMTTKPRILVADDEEFNLDNILFHLTEAGYDVIGAEDGAVAFEK
                     LKSSGPVDVIVLDRIMPNMDGMELLRAIKAEDEFRDIPVVMQTAASEPEEVLEGIKAG
                     VYYYLTKPYEQEMLLGIVGSALRDTQSNKALQESVRRNRSILGLMVNSCFRFRTLDEA
                     KNLAAYVANCFPEPDRAIYGLHELLINAVEHGNLGITYSEKTKLIFDHQWDDDVQRRL
                     GLAENRTKFAHISFQSTADAIVVIIKDEGDGFDWEKYLELSPDRVTDPHGRGIATSRM
                     MSFHSLEYLGSGNEVRCTVKL"
     gene            2501..2689
                     /locus_tag="VNR65_17105"
     CDS             2501..2689
                     /locus_tag="VNR65_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20438.1"
                     /translation="MTLDRNDDQACGSDLRDLSQKLRAVANDVRHLSKDRTDFPEKVQ
                     ALRGELEFIISRLRHSAL"
     gene            3041..3835
                     /locus_tag="VNR65_17110"
     CDS             3041..3835
                     /locus_tag="VNR65_17110"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWJ20439.1"
                     /translation="MLLSLWTWFEIALCAVVGFLMQLVLAPFTLPFDRRRIIVGRCFR
                     LAGVTAARLTPFWRFGVYGDVPRRIDGRTVFVSNHESQADPFLISFLPWEMKWLGKAS
                     LFRIPIAGWSMALAGDIPIHRGEGRSAKGAMDRCKQWLERGVPVIFFPEGTRSPDGKM
                     GPFKDGAFKLALDSGAQIIPIAVCGTRKALPKHSWKFGFTRALVAVGTPIPTIGRDAS
                     DLEAIKAEARAQIEALRARMLPLTGAGGLRNKAEVERSALKVSTGV"
     gene            complement(3943..>4254)
                     /locus_tag="VNR65_17115"
     CDS             complement(3943..>4254)
                     /locus_tag="VNR65_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HWJ20440.1"
                     /translation="EAPTGNGHAIDYETAGAPALHIRVQELFGLGHHPSIAGGKLPLT
                     LHLLSPAHRPLQITRDLPGFWKGSWAAVKAEMKGRYPRHPWPDDPASAVPTSRAKPRG
                     T"
BASE COUNT          831 a         1217 c         1275 g          931 t
ORIGIN      
        1 agctttctct tcgtcgcgag aatttcagtc ttcgggatct gctcggcggg gtcatgtctc
       61 agcttttctc tgatgccgag cagaaggggc tgaacctgag gtcggacacg agcgcttata
      121 ttcccgatca gctctatggc gatcccttga ggatcagcca gtgcttgctc aactacctaa
      181 gcaacgcggt caagtttacg ccccaggggt ctgtgatcgt tcacgttgac ctggagaagc
      241 gaagcggcgc gggcttattg ttgaattttg agatcgaaga caccggcatt ggcgttgagc
      301 cggaagcgct gacacggctt ttttcgccat tcgagcaggt ggacacctcg atgacccgga
      361 agttcggcgg aacggggctc gggcttgcga tcacgcgacg gctcgccatg ctcatgggcg
      421 gcgaggccgg aggaacgagc gctcccggca aaggcagccg cttctggttt tccgcaatgg
      481 tcgaaccggc gaaggcttct tcggtgtcgg cgcgcctgaa gagcaaatcg gccgccaaac
      541 cgggcgaaac gttcggtgac gcgcgtgtgc ttgtcgtcga ggatgttgag ttgaaccgcg
      601 aaatcctgtt ggacatgctg agggaggacg gacttaaagc cgatactgcc gagaatggtc
      661 aggaggccgt gaccaaagcg gcctcgaatc cttacgatat cattttcatg gacatgcaga
      721 tgcccatcat ggatggtctc accgcgacga tgacgatccg tcgcatggcg gcccataggg
      781 aaacgccgat catcgccgtg accgccaacg ttttcgagaa gtctcgcgac gagtgtctgg
      841 ctgcgggtat gaacgatttt ctatgtaagc cgctcgatcc agatgatttg catgctgcac
      901 ttcatcaatg gctgagtaag gaaccggcgc cggttgcagt cgagtcccat ccgaaggctc
      961 gaggcgagaa ggttgagggc ggagacctcg ccagccttcg ccgctgtctt ggcgagatcg
     1021 ccgatatcga tctggactgc ggcttaaggc ggatcaagca gccggaacgc tacgttcgct
     1081 acttgcgcca atatggtgac aaatacgaag gcagcttcag ccatctgcgg gacttgctcg
     1141 ccaaaggcaa aacggcggat gccgcaagct ttgcgcattc cctcaaaggc gcttccgctc
     1201 aactaggcgt ggtgggggtc gccaagcagg cggaactgtt gcaggccgcg atcaagaacg
     1261 aggcggaaga gggttcgatc gaggctttgc ttacgcagtc cgaagaacga tgcgccgtcg
     1321 tctgggcatc gatccaaaaa ctggaggttt ccaccccggc tcattaagct caacgctcgc
     1381 ctcctcaaag tttgacagtg catcgcacct cgttgccgga gccgagatac tcaaggctat
     1441 ggaacgacat cattcgagac gtggcgatcc ctcgcccatg gggatcggtt acgcggtctg
     1501 gtgaaagttc gagatatttc tcccagtcga agccatcgcc ctcgtccttg atgatcacga
     1561 cgatggcgtc cgcagtcgat tgaaatgaga tatgagcaaa tttggttcga ttttcagcca
     1621 accccaatct tcgctgcaca tcgtcgtccc attggtgatc aaagatcagt tttgtcttct
     1681 cggagtaggt tatgccgagg ttgccgtgct cgacagcgtt gataagaagc tcatgcaacc
     1741 cataaatggc cctgtcaggc tcggggaagc aattggcgac ataggccgcg agattttttg
     1801 cttcatccaa ggtccgaaag cgaaaacagg aattgaccat caggccaaga atgctccgat
     1861 ttcttcgaac ggattcctgt aaggctttat tgctttgggt atctcgtagc gcggagccga
     1921 cgatgccgag caacatctcc tgctcgtatg gcttagtcag gtaatagtat acgcccgcct
     1981 tgatgccctc gagcacttcc tcaggttcgg aagccgccgt ctgcatcacg acgggaatgt
     2041 cccggaattc atcctccgct tttatcgctc gtaagagctc catgccgtcc atgttcggca
     2101 tgatgcggtc cagaacgatg acgtcgaccg gtccggaact ctttagcttt tcgaaggcga
     2161 cggcaccgtc ttctgcgccg atgacgtcat aacccgcctc ggttagatgg aagagtatat
     2221 tgtcgagatt gaattcttcg tcgtcggcga cgaggattct tggcttggtt gtcatcatgt
     2281 tgttgcccgc acgacattta tgatcatagg gcgagcttaa acgatgtcgc ttttgtgagg
     2341 cctgcaaccg ggccggctgc gcgatcacgg tccggcggaa atccccggca gttcgaagcc
     2401 ggatattttt cttctccggt atggaacaaa tacaggcttt cgctcgttat tgaccctcgg
     2461 tgaagctctt tcttcgctgt gatagggcac ggagtggttt atgacgttgg ataggaatga
     2521 tgatcaagcc tgcggatctg atcttcggga tttaagccag aagctgcgtg ccgttgcgaa
     2581 tgatgtccgg catctttcca aggatcgcac cgacttccca gaaaaggtgc aggctcttcg
     2641 tggagagctg gaattcatta tctcccgttt gcgccatagc gcactgtgaa gacgagctga
     2701 gcatccaacc aggtgcgttc tcgcgctcat tttcttaaaa cgcttatccc ttaccgcatt
     2761 gagctttccc gaaggctccc cgccgggaag ggctcggaga gaacgccaag ccgccgatca
     2821 tcgcttgctg catccaaaaa aagctcgcgg gaactttgat tttatttcgc gcctcttggg
     2881 gaagccctcg tggcagtctg ctcgcgaacc cgaagcacag cgccctgatt gcccgttgca
     2941 gacgctatct cgcggcgatc ccccgaacgc ggttgacgct cgtcggcaca cgcaatacag
     3001 gttcacatca tgaactgaaa tatgcgcggt catggggcag atgctgcttt cactctggac
     3061 gtggttcgag atcgcgctct gtgcggtcgt gggtttcctc atgcagttgg tgctcgcgcc
     3121 cttcaccctg ccatttgacc ggcggcgtat catcgtcggg cgctgcttcc gcttggccgg
     3181 ggtcaccgcg gcccgcctga cgccgttctg gcgctttggc gtgtacggcg atgttccgcg
     3241 ccgtattgac gggcggacgg tgttcgtctc caaccacgag tcgcaggcag accccttcct
     3301 catctcgttc ctcccctggg agatgaaatg gctgggtaag gcgtcgcttt tccggattcc
     3361 gattgccggt tggagcatgg ccctggcggg cgatatcccg atccaccgcg gcgagggccg
     3421 ctcagccaag ggcgccatgg accggtgcaa gcagtggctt gaacgcggcg tgccggtgat
     3481 ctttttcccc gaggggacgc gctcgcccga tggcaaaatg gggccgttca aggatggtgc
     3541 tttcaagctc gccctcgaca gcggcgccca gatcatcccc atcgcggtgt gcggcacccg
     3601 caaggctctt ccgaagcatt cctggaagtt cggctttacc cgcgctctcg tagcggtagg
     3661 cacccccatt cccaccatcg ggcgcgacgc gagcgacctg gaggccatca aggccgaggc
     3721 ccgggcgcag atcgaggcac ttcgggcacg gatgctcccg ctcaccggcg ccggggggct
     3781 tcggaacaag gccgaagtgg aaaggtccgc gctcaaggtg tcaactggcg tgtgaccgtc
     3841 tgcaatacgg ctccactaat ggggcgacga actcttgcgg gcggaccggc gtcttccgcc
     3901 acttaagctg cgaagctttc tcgaattcgc atccctgaag catcaggtcc cgcgcggctt
     3961 ggcccgcgat gtcggaacgg ccgaagcggg atcgtcgggc cagggatggc gcggatagcg
     4021 ccccttcatc tcggccttaa ccgccgccca cgaccccttc cagaagcccg gaagatcgcg
     4081 cgtgatctgt agtggccggt gtgcgggcga gagcagatgc agtgtgagcg gcagttttcc
     4141 gcccgcgatc gaaggatggt gccccagccc aaacaactcc tgcacgcgga tatggagcgc
     4201 gggtgcgccc gcagtctcgt aatcgatggc gtgaccattg cctgtggggg cctc
//
LOCUS       DATMGX010001042         3371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_58248, whole genome shotgun sequence.
ACCESSION   DATMGX010001042 DATMGX010000000
VERSION     DATMGX010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58248"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1624)
                     /locus_tag="VNR65_17120"
     CDS             complement(167..1624)
                     /locus_tag="VNR65_17120"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20441.1"
                     /translation="MGLIGIFAALAFLIFLAFRGWSILLLAPMAGLIAATFAAEPLLA
                     SWTQTFMLNAAQFMAQFFPLFLLGALFGKLMEDSGSVSSIANYMTLKLGPQRAILAVV
                     LAGAIVTYGGVSLFVAFFVLAPMATELFRSAGVPRRLMPAAIALGTSTFTMSAMPGTP
                     SINNAIPMPFFGTTPFAAPGLGIIASAIMLVFGMWWLKRAEVKARAAGEGFGPDESKS
                     AERLAAKDPIVRERATTAHEFDPAEIDRSASAERGPPILLAALPIFVVIAVNLLMSFI
                     VLPRMNVSFLAEPQWGATSLSAVGGVWSVIVALLSAVVATVAINFRSVPELRATMTAG
                     SNAAALPIVSVASLVGFGAIVAALPSFALVREWVLGIGGGPLVSLAIATNILAALTGS
                     ASGGLTIALDALGPTYMKLAAETGLDPALMHRVAVIGSGTLDSLPHNGAVVTLLSVCG
                     VSHKDGYLDIVMAAIVGALIALVVVILLGSQVGSF"
     gene            complement(1628..2650)
                     /locus_tag="VNR65_17125"
     CDS             complement(1628..2650)
                     /locus_tag="VNR65_17125"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWJ20442.1"
                     /translation="MERERVLVDLALQGGGSHGAFTWGVLDRFLEESWLEIEGVSGTS
                     AGAMNAAVLADGFAAGGAEGARTALETYWRHVSEAARFSPFQRTLLDRMLGRWSLDHS
                     PMFIMMDTVSRMFSPYQLNPSGSNALAPILDKEIDFERLQNSPVKVFITATNVRTGRP
                     RVFRNAEITPEVLMASACLPTIFQAVEIDGEPYWDGGYSGNPTLAPLVGELKSDDTIL
                     IPINPVERPGTPKTPHEILNRLNEVSFNAVLIKELQMMAMLRKNAGNLSNGEGAEWAR
                     MRVHIVRNPVMNKLGFSSKLNAEWDFLSMLKEEGRRSADDFLIAHGDDLGKTSTLDID
                     QLLAEF"
BASE COUNT          725 a         1020 c          999 g          627 t
ORIGIN      
        1 tggcggagca gcgggttgct gagcaaagat agcgccggta tcgacccgcc acagaatacg
       61 tccaagcgtt ggcgaagcca tgcacgtttg cttaggccgc cagagcaata tgccgaaatc
      121 aaaagcctcc cgcattttac atcgctgtgc aggtctctca cacacgtcag aagctgccga
      181 cttgagatcc cagcaaaatt acgactacaa gtgcaattag cgcgccgaca atggcggcca
      241 taacaatatc gagatagccg tccttgtggc tcaccccgca taccgagagt agtgtgacca
      301 ccgcgccgtt gtgcggtagg ctgtcgagcg ttcccgaacc gatcaccgcg acgcggtgca
      361 tcagagccgg atcgaggcca gtctcagcgg cgagcttcat ataggtgggt ccgagcgcgt
      421 cgagcgcgat ggtgagaccg cccgatgccg aaccagtgag cgcggcgagg atattggtag
      481 cgatggcgag agaaaccagc ggtcctcctc ctataccgag cacccattcg cgtactagcg
      541 cgaatgatgg aagtgcggcg acgatcgcgc cgaagccgac gaggctcgcc accgagacga
      601 tcgggagcgc cgcggcgttg gagccagcgg tcatggtcgc cctcagttcg ggcacgctgc
      661 ggaaattgat ggcgaccgtg gcgacgacag cgctgagcag cgccacgatc accgaccaaa
      721 cgccgccgac tgccgacagg ctggtggccc cccactgcgg ctccgccaag aacgagacat
      781 tcatcctcgg aaggacaata aacgacatca gtaggttgac ggcgattact acgaagatcg
      841 ggagcgcggc gaggaggatg ggaggaccgc gttcggccga ggcactgcga tcgatttccg
      901 ccggatcaaa ttcgtgcgcc gtagttgcgc gctcgcgcac gatgggatcc ttcgccgcca
      961 acctttcggc cgacttagat tcgtccgggc caaagccttc gcccgcggcc cttgccttaa
     1021 cctccgctcg cttcagccac cacataccga agaccagcat gatggcagaa gcgatgatgc
     1081 caagccccgg cgcggcaaac ggcgtcgtgc caaagaacgg catcgggatc gcgttgttga
     1141 tggaaggcgt gccgggcatc gccgacatgg tgaaggtgga ggtcccaagc gcaatcgccg
     1201 ccggcatcag tcgccggggc acgccggccg agcgaaacaa ctcggtcgcc atcggagcca
     1261 gaacgaagaa ggccacgaat aggctgactc cgccatacgt cacaatcgct cccgccagca
     1321 ccaccgcgag gatcgctcgc tgcggtccca gcttgagcgt catgtagttg gcaatggaag
     1381 aaaccgagcc tgaatcctcc atcagcttcc cgaacagcgc gcccaacagg aacaggggga
     1441 agaactgcgc catgaactgc gcggcgttga gcatgaaggt ctgcgtccag ctcgcaagca
     1501 acggctcggc tgcaaaagtc gcggcgatca ggccggccat cggtgcgagc agaaggatgc
     1561 tccacccacg gaaggcaagg aaaatgagaa aagcgagcgc ggcaaagatg ccaataagac
     1621 ccacggctca gaactccgcc aagagttggt cgatatcaag cgtagacgtc ttgccaaggt
     1681 cgtcgccatg cgcaattagg aaatcatccg cggacctacg gccttcctcc ttcagcatgc
     1741 tcaggaagtc ccactcggcg tttagcttcg aagaaaatcc aagcttgttc atgacgggat
     1801 tgcgcacgat gtggacccgc attcgagccc attcggcgcc ctctccgttc gacagattgc
     1861 cggcattttt gcggagcatc gccatcatct gtagttcctt gatcagcacc gcattgaacg
     1921 agacctcgtt caggcggttg aggatctcgt gcggcgtctt cggcgtgccg gggcgctcca
     1981 ccgggttgat gggaatgagg atggtatcgt cagacttgag ttcgcccacc agcggcgcca
     2041 gcgtcgggtt gccggaatag ccgccgtccc aatagggctc gccgtcgatc tccaccgcct
     2101 ggaatatggt gggcaggcat gccgaggcca tcagaacttc cggcgttatc tcggcgttgc
     2161 gaaacacccg cgggcgtcca gtgcgcacat tcgtcgcggt gatgaagact ttgacgggag
     2221 aattttgtag ccgctcgaag tcgatctcct tgtccaatat cggtgccaga gcgttgcttc
     2281 cgctcggatt gagctgatag ggagagaaca tacgggacac cgtgtccatc atgatgaaca
     2341 tcggcgaatg gtcgagcgac caccgtccaa gcatcctgtc caggagtgtc cgctgaaagg
     2401 gactgaagcg tgccgcttca gacacatgac gccaataggt ctctagcgcc gtgcgcgcgc
     2461 cctcggcccc cccggcggca aagccatcag ccagaaccgc ggcgttcatt gcgccggcgg
     2521 acgttccgga aacgccttcg atttcgagcc agctttcctc aagaaagcga tctaggacgc
     2581 cccacgtaaa tgccccgtgg gagcctccgc cctgcaaagc gaggtcgacc aatacccgtt
     2641 ctcgttccat ttgaaactcc cgcttcgtgc cgatgggtgc ccacccggac gccacttgtg
     2701 gcgacagctc ggacgattgt aaccgttaag acgctatagg ccactgcgct ttttgcatcg
     2761 cagcaaagcg ctatggcgga gggatacgtg cctacgcgat catgaaaatt attcagtact
     2821 gaaccaattt tggtccatta ggcacatctg ggctttgact ggccgaccgg caagatcgcg
     2881 gcccgttttg ctttcccgga cgacaggcga aatacctacg gcgacaatac cgccatcctc
     2941 gaagacggcg ctcgtctggc tttgataggc acaagttgct gcccttaggg acatggatta
     3001 acgagaacgg gcgcgttaat agcctcggac ttcaggctga aaacccctta gctggccaga
     3061 tattgcaaat ctgtgggaat tgagtttctg tcgcatgcga gagcttggcc atcattgggc
     3121 cggcatcgac cagcctgatt tctccatgcg atggatgcct gatgacgacg ttcgaaacgc
     3181 caggccttct tactttcacc atttcgatca tcgtgttctt tatcggcagg ggtcttaacg
     3241 gtctgatcgc gtcccttcgg cggtggaaca ttcccgaggc ggtcaccggc ggcctgctcg
     3301 cggcaccaag atgcgcgacc gcggcgtcga cgtcgccgct taccttttcc tggtgcttgc
     3361 taacgcgttg a
//
LOCUS       DATMGX010001043         2283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_283681, whole genome shotgun sequence.
ACCESSION   DATMGX010001043 DATMGX010000000
VERSION     DATMGX010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283681"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /gene="sucC"
                     /locus_tag="VNR65_17130"
     CDS             complement(<1..1034)
                     /gene="sucC"
                     /locus_tag="VNR65_17130"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWJ20443.1"
                     /translation="MNVHEYQAKAILRDYGVPVPRGQAAFAPEEAVSVAKELGSPITV
                     VKSQIHAGGRGKGHFKEPEAGDKGGVRLAKSIDDVRLFADQMLSHTLVTLQTGPAGRL
                     VKRLLVEEGAAIARELYLSFLIDRVTSRVAIIASTEGGMDIEEVAHKTPEKIVTIHID
                     PATGFSQFHGRQVAFALGLKDGQIRQCVNLIGNLYRAFTEKDMSLLEINPLIVTDKGD
                     LVCLDAKLNFDGNALYRHPDILNLRDLDEEDPAEVLASRYDLSYVKLDGSIGCLVNGA
                     GLAMATMDIIKLYGSEPANFLDVGGGATKEKVTEAFKIILSDPNVKGILVNIFGGIMR
                     CDIIAEGIIAA"
     gene            complement(1117..2091)
                     /gene="mdh"
                     /locus_tag="VNR65_17135"
     CDS             complement(1117..2091)
                     /gene="mdh"
                     /locus_tag="VNR65_17135"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWJ20444.1"
                     /translation="MARAKIALIGSGMIGGTLAHLIGLKELGDVLLFDIMEGLPQGKA
                     LDIAESSPVDGFDGAFTGVNDYADIKGADVVIVTAGVPRKPGMSRDDLLEINLKVMEQ
                     VGAGIKKYAPNAFVICITNPLDAMVWALQRVTGLPKNQIVGMAGVLDSARFRYFLARE
                     FDVSVEDVSAFVLGGHGDTMVPLPRYTTVAGIPLPDLIKMRWITHERLNEIVQRTRDG
                     GAEVVKLLKTGSAFYAPAASAIAMTEAYLKDKKRVLPCAAHLTGEYGVKGLYIGVPCV
                     IGAKGMERVIEVDLNAHERLDFKRSVEAVQNLVEACVRIAPGLQQPSA"
BASE COUNT          460 a          691 c          661 g          471 t
ORIGIN      
        1 gctgcgataa tgccttccgc tatgatgtcg cagcgcatga tgccgccgaa gatgttgacg
       61 agaatgccct tcacgttcgg gtccgacaga atgatcttga aagcctccgt gaccttctcc
      121 ttcgtcgcgc cgccgccgac atcgaggaag ttcgctggct ccgagccata aagcttgatg
      181 atgtccatgg tggccatggc aagaccggcg ccgttcacga gacagccaat actaccgtcc
      241 agcttcacat aggaaaggtc gtagcgggac gccaaaactt cagccgggtc ttcctcgtcc
      301 agatcgcgca aattcagaat gtcggggtgc cggtatagag cgttgccgtc gaagttcagt
      361 ttggcgtcca ggcaaacgag atcgccctta tcggtgacga tgagcgggtt gatttcgagg
      421 aggctcatgt ccttctcggt gaacgccctg tagaggttac cgatcaggtt cacgcattgc
      481 cggatctggc cgtccttcag gccgagcgcg aaggcgactt gccggccgtg aaactgcgag
      541 aagccggttg ccgggtcgat gtggatcgtg acgatcttct caggcgtctt gtgagccacc
      601 tcttcgatgt ccatgccgcc ttccgtcgaa gcgatgatcg caacgcgaga tgtaacgcgg
      661 tcgatcagga aggagaggta aagctcgcgc gctatggcag cgccctcttc gacgagcaac
      721 cgcttcacga gccggccggc cggccccgtt tgcaaagtca caagcgtatg cgacaacatc
      781 tggtccgcaa acagacgcac gtcgtcgatc gatttcgcaa ggcgcacgcc gcccttatcg
      841 cctgcctcgg gctctttgaa atgccccttt ccgcgccctc cggcatgaat ctgcgatttc
      901 acgacggtga tggggctgcc caattccttc gcgaccgaaa cggcctcctc tggggcgaag
      961 gctgcctgcc cgcgcgggac gggaacgccg taatctctga ggatcgcttt ggcctgatat
     1021 tcgtgcacat tcatggggac agctcttatc gtgagagagg aggcaaagat tggtatccgg
     1081 aaaaaagagg ccgccgaaaa cggcggctct gataccctac gctgagggct gctgaagacc
     1141 cggcgcgatc cgaacgcagg cttcgacgag gttctggacc gcttccaccg agcgcttgaa
     1201 gtcgagccgc tcgtgtgcgt tgaggtcgac ttcgatcacg cgctccatgc ccttggcccc
     1261 gattacgcag gggacgccaa tgtaaaggcc cttcacgccg tactcgcctg tcaagtgggc
     1321 tgcacatggc agaacgcgct tcttatcctt cagatacgcc tcggtcatgg cgatggctga
     1381 agccgcgggt gcgtagaagg ccgagcccgt cttcaaaagc tttacaacct cagcgccgcc
     1441 gtcgcgggtc cgctgcacga tttcgttcag ccgttcatgc gtaatccagc gcatcttgat
     1501 aaggtcgggg agaggaatgc ccgcaaccgt agtgtagcga gggagcggca ccatcgtatc
     1561 cccatgcccg ccgagcacga acgccgaaac atcctcgacc gacacatcga attcgcgagc
     1621 gagaaaatag cggaagcgcg cgctgtcgag cacgccggcc atgcccacta tttggttttt
     1681 cggcagtccg gtcacacgtt gcagcgccca caccatggcg tcgagcgggt tcgtgatgca
     1741 gatgacgaag gcattgggcg catatttctt gatgcccgcg ccgacctgct ccatcacctt
     1801 caaattgatt tcgagaagat cgtcgcggct catgccaggc ttgcgcggca cgccagcggt
     1861 cacgatcacg acatccgcgc ccttgatgtc ggcatagtcg ttcacgccgg tgaaagcacc
     1921 gtcaaagcca tcgacaggcg aggattcggc aatgtccaac gccttaccct gcggcaagcc
     1981 ctccatgata tcgaaaagca acacgtcgcc cagctctttg aggccgatca gatgggccag
     2041 cgttccgcca atcatacctg aaccgatgag tgcgattttg gcccgtgcca tgccgttctc
     2101 cttgacattc gtgcttgcgt tcccagcgcc cctgggtact cgcatttcca ctgcgctgca
     2161 acatatgctc aagtttagca tcgcgcgcgg gcgatcttaa gcggaaatgc gccgcagcct
     2221 tctggctatg caggttagcc gggttttatg cttctgtgcg gagatcgcgt aattggaaaa
     2281 tct
//
LOCUS       DATMGX010001044         2222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_12054, whole genome shotgun sequence.
ACCESSION   DATMGX010001044 DATMGX010000000
VERSION     DATMGX010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12054"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            606..803
                     /locus_tag="VNR65_17140"
     CDS             606..803
                     /locus_tag="VNR65_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20445.1"
                     /translation="MQNIVDDELHTMWKPGTTAKELYKLYVAFGPDPFVGPLNPKDPD
                     VEFSAWTYAKERCKELVRLGD"
     gene            complement(890..1132)
                     /locus_tag="VNR65_17145"
     CDS             complement(890..1132)
                     /locus_tag="VNR65_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20446.1"
                     /translation="MPQVKGDDEAYEDRLVILEELGIEENALEHDESTDEVSASSDEA
                     AIKRFFARAFREWAEGNVEGTAQEIFDAVVTLLDSD"
     gene            1477..2061
                     /locus_tag="VNR65_17150"
     CDS             1477..2061
                     /locus_tag="VNR65_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20447.1"
                     /translation="MSDQVVGVVPRSASPVRRGYVVAAVLALAMMVLAIQYGSDWLLN
                     FLHVMTGALWTGIDLFMGFVVGPVLKRLPLDARRAVISGLVPRTLFLMPTVSIITSTT
                     GWFLAKRMGFLDVGYPGFHWVIAALVIVTILTIQGTGILLPTNLRLYFELQKPNPDSA
                     KIGRWMRWFVGVVAIQGTMQVAIFVVMAKFATGL"
BASE COUNT          437 a          666 c          621 g          498 t
ORIGIN      
        1 ccgcaaatgc ccgccgccca ggcaactccc ccgcgggtgc aacggtcgcc gaccgggacc
       61 tatcgaaagg ttaatcgccg accacctgac tcccccaacg aggtatccga tggatagcaa
      121 cgctctttcc ccgccaaagc ggcaataaga gaactcgtgg ttaatccatc gcttgcctcg
      181 tgtggtttgc ggcctatatc agctgtgtgc catgcggtac cacctatcgc ggcctcgcaa
      241 aaccgcttgc agcggtgaaa tcgaaactcg gctgcttcgg ctgaagaagc ccggctcgag
      301 tttggccgcc cacccgaaac cgcgatatgc gtctaagggt caatcaccaa cttgacgctc
      361 ggccagccgc gatcttcgcg gggtgcgtta cactgcgctc tcagagatcg aatagccgcg
      421 atgtgctcag cacagatgtg cgtgcttcat tccaactttc aacaacaaga ttttatcgcc
      481 cacctcgaaa aaggagaaac gatgccgaat tatagagtac tgattaatga caacgcccat
      541 tacatggatg aaagcgagcg cgccgatcat ggtatatttg ccaacgcgga tgaggccata
      601 gccgcatgca aaatatcgtt gacgacgagt tacatacgat gtggaagccg ggcacgaccg
      661 cgaaggagtt gtataagctt tatgtcgcgt ttgggccgga cccatttgtc gggccgctta
      721 atccgaaaga tcctgacgtt gaattctcgg cttggacgta cgccaaggaa cgatgcaaag
      781 agcttgtaag gttaggcgat tagcgtagtc cgctttgctc ccatgagccg acatcgtctg
      841 tcgcggcgtt caaggccatg agaccgcgcc gagcgagcag ctcttcttgt taatcgctgt
      901 caaggagagt aactacagcg tcaaagattt cttgggctgt tccttctaca tttccttcgg
      961 cccattcccg aaacgctctg gcaaagaacc ttttaatcgc cgcttcgtcg cttgaggcac
     1021 ttacctcgtc agtggactca tcatgctcca gcgcattttc ctctatacct aactcttcta
     1081 agatcactaa ccgatcttca taagcttcat catcgccctt cacctgaggc atttccattc
     1141 tcccgtcccg agatgagaaa ctcctttgcc cgcgctcggg cttgagcacg cggtaacgtg
     1201 tagatttcgc gcctgtagct gttgcccggc ttgtgccggt cggccatgcg atccggcaga
     1261 tcgcgcggtc gataagtcat gccaactcga tacagatgca atataggcgg ctcagcgggt
     1321 cgcgccaacg ttggacgaga tctcgcctag atcggcgggc aaaaagagga acttatggag
     1381 tgaccgaagc gggccccggt tgaccctcgc agccgcggca cctaccgtcc gtggttgcct
     1441 tctccgaatc cactgaattt ttcgcggagc cttaccatga gcgatcaggt cgtcggcgtt
     1501 gtgcctcgct cggcctcgcc ggtccgccgc gggtatgtcg tcgctgcggt gctcgctctg
     1561 gcaatgatgg ttctcgcgat ccaatacggc agcgactggc tgttgaattt cctccacgtg
     1621 atgaccggcg cgctgtggac cggcatcgac ctgttcatgg gctttgtggt cggaccggta
     1681 ctcaagcggc taccgctcga cgcccgccgc gccgtgatct cggggctggt cccgcgaacg
     1741 ctgttcctga tgccgacggt cagcatcatc acctcgacca ccggttggtt tcttgccaaa
     1801 cgaatgggtt ttctcgatgt cggctacccg gggttccatt gggtcatcgc agcgctcgtc
     1861 atcgtgacga tcttgactat ccagggcacg ggaatcctgt tgccgacgaa tctgcgcctc
     1921 tatttcgagt tgcagaagcc gaacccggac agcgccaaga ttgggcgctg gatgcgctgg
     1981 ttcgtgggcg tggtggcgat ccagggcacg atgcaagtcg ccatattcgt ggtcatggcg
     2041 aagttcgcga ccgggctgta gcgccctacc ccacctcgac gatatcttcc gggaactggt
     2101 gcagccgctc gccaggccgc atccacgacc gtggttagac tgctggcgcg cggggcgctg
     2161 ttcacttgtg tcggcttctc gcgctggagg ctctcaaaga ggcgttcccc tgcgctgatg
     2221 gg
//
LOCUS       DATMGX010001045         3110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_108394, whole genome shotgun sequence.
ACCESSION   DATMGX010001045 DATMGX010000000
VERSION     DATMGX010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108394"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VNR65_17155"
     CDS             complement(<1..524)
                     /locus_tag="VNR65_17155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWJ20448.1"
                     /translation="MRTFASLALLASGGDCAAGSAINRFRNIDVRKWELRTMKLARIA
                     ITVTFLLGALMIGANPALAQQVTGTLGSPSATTTIPGEQLPPPAPKFGGVIKENRPGS
                     TPWWPPTVVPPKGAPNVLLIMTDDSGFGIPSTFGGVIPTPSMDRIANAGLRYNRVFST
                     SLCSPTRAAIITGR"
     gene            668..1696
                     /locus_tag="VNR65_17160"
     CDS             668..1696
                     /locus_tag="VNR65_17160"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HWJ20449.1"
                     /translation="MAASPIALVRSTVLTPIVELLDQERVPYEALFEAAKLSPRLIMR
                     PDDLIPLAQGCALLEKIARATGLEDIGLLAASKIKIPAFGQYGDLACGAATLFEALRS
                     LIAGVTAFSTGERLSLEWRSERLLLCHRFAMRPTPGTRHGDSYSLVVMIKSVQLALGS
                     EWKPDLILLPANERIRKRQYEAFFKTNISFENDVWAISIPPSRLADPIRKAERPASPN
                     TPQPASPEPFTPDNDLIGSLRAMIGPSLRLSYPDIGHAAGLAGISVSTLQRRLREYGL
                     TYSDLVETERLRLAIELLAQEDIKICDVAAELGYTESANFIRAFRKWTGLTPSKFRSN
                     QLGPRSGR"
     gene            complement(1749..1940)
                     /locus_tag="VNR65_17165"
     CDS             complement(1749..1940)
                     /locus_tag="VNR65_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20450.1"
                     /translation="MFPVTWNWAIDVSGNPVTIELTAKKLNLHIYSGAPLHGAWNGFS
                     SGWSIYNSGYWGAVLCSAE"
     gene            complement(2598..3014)
                     /locus_tag="VNR65_17170"
     CDS             complement(2598..3014)
                     /locus_tag="VNR65_17170"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HWJ20451.1"
                     /translation="MTEHHEESTKAPPDTPSRLSLARTYLSHERTMLSWVRTATSLIT
                     FGFSIQQFFRITKGATFQDKGLVGPEEFGLIMIVIGLLALLLAAVDHRAHINALRAQY
                     PVKDGYNNLPPSQATFLAALIGMLGLLAFSSVLFGK"
BASE COUNT          690 a          882 c          906 g          632 t
ORIGIN      
        1 ttgcgcccgg tgataatcgc ggcgcgcgtc ggcgagcaca gcgaggtgga gaatacgcgg
       61 ttgtagcgca gcccggcatt cgcgatgcgg tccatggacg gtgtcgggat gacgccgccg
      121 aacgtgctcg gtatgccaaa cccggaatcg tcggtcatga tcagcaggac gtttggcgcg
      181 cccttgggcg gcaccacagt cggcggccac catggcgtgg atccgggcct attctcctta
      241 atcacgccgc cgaatttcgg agccggcgga ggcagctgct cgccggggat ggtcgtcgtg
      301 gcgctgggcg aacccagggt gccggtgacc tgctgcgcga gggccgggtt cgcgccgatc
      361 atgagcgcgc ccaagagaaa cgtcacggtg attgctattc gagcgagctt catggtgcgt
      421 agctcccact tccgtacatc gatgttgcgg aaacggttga tggcactgcc agctgcgcaa
      481 tcgccccccg acgccaggag ggcaagggag gcgaacgtac gcatccattt atctgtacgc
      541 atcgataaac ccggacaaaa cgttgcgaca ttaacgtttc ttaacatacc tcgctttgga
      601 gagggttatc atctcaggtc aaacagggtt gtttttctct ttttgatcat gctgaacgga
      661 agacccgatg gccgcgtcgc cgatcgcctt ggtacgttcg accgtgctaa ctccgatagt
      721 cgagctcctc gaccaggaac gggtgcctta cgaggccctt ttcgaagcgg cgaaactgtc
      781 gccgcggctg atcatgcgtc cggatgacct tattcccctg gcgcagggtt gcgctttatt
      841 ggagaagatc gcgcgcgcaa caggactcga agacatcggc ctcttggcag caagtaaaat
      901 caaaattccc gctttcggac agtatgggga tctggcttgc ggggcagcga cacttttcga
      961 agcgctccgc agccttattg ctggtgttac cgcgttcagc acgggcgagc gcctttccct
     1021 ggaatggcga agcgaacgcc tgttactttg tcacagattt gcaatgcgcc cgaccccagg
     1081 aacgcgccac ggagactcct actcgttggt ggtcatgatc aagtccgtcc agctcgcgtt
     1141 gggttcagag tggaaaccgg atctgatctt gctcccggca aatgagcgga tcagaaaacg
     1201 ccagtacgaa gccttcttca aaacgaatat ctcgttcgag aacgatgtat gggcgataag
     1261 cattcctcct tcgcggcttg ccgatcccat tcgcaaagcc gagcgtccgg cctcaccaaa
     1321 caccccgcaa cctgcaagtc ccgagccgtt cactccagat aacgatctta tcggttcttt
     1381 gcgtgcgatg atcgggccga gcctccggct ttcttatccc gatatcggcc atgccgccgg
     1441 attggccggc atcagcgtca gcaccttgca gcgccgcttg cgggagtacg gtctcaccta
     1501 ctcagacctt gttgagaccg aacgtctacg gctcgcgatc gaattgctgg cccaagagga
     1561 catcaagatt tgcgatgtcg ccgcggagct cgggtacacc gagtccgcaa atttcatacg
     1621 cgcgttccga aaatggacgg gtctgacgcc gtccaagttc cgtagcaatc aactgggtcc
     1681 gcgatctgga aggtgattgg cacagacgac ggtgtcaccg ccgccgcctc acaacctttg
     1741 gagcgcctct actcagccga gcaaagaacg gcgccccagt aacccgaatt gtaaatcgac
     1801 cagcccgagg agaagccgtt ccaggccccg tgcagcggcg cgccactgta gatatgcaaa
     1861 ttcagtttct tcgccgtcaa ttcaatcgtg acgggatttc cgctgacatc gatagcccag
     1921 ttccaggtga cgggaaacaa gactggatcg tcccccccag ggctgaaata aatcgcaacc
     1981 gaggctaccg ggaatgtccg gctatgatcc cacgaagtta catgattgct tgagttgtta
     2041 tccagtttcc agggcagaaa ctcacacgcg gagttggcgt tcaatttgag atttggggga
     2101 ggagtttgat aatgagggac gattttccga ttgccccctt ctgtcgtctg cgccaaagcg
     2161 caaggtaccc ataaacagaa gaggcaaaca gcgagcttca tcttcggcaa cgcttcctcc
     2221 ctactggaca aacggcctaa gtaatatgct tgcggccgcc ggacacaaag aggcggcgga
     2281 ggcacgcggg acagcgtgac tattcgcaat ccgatgcgtg agggctttga gcgttgtcaa
     2341 tgatgttcaa gaaatgtgat taagtatccg gcgcggaaaa cgggagaagt agggggcccg
     2401 ctgccagaac agctttccaa tgccaatcag cacagccgtt gccaacggca ggtttattga
     2461 tacggagcca caaaatcagg actgatccgt ccgcgtgtgc ccgtggccca ggccgctccc
     2521 gttaagcacg gagggtgaac ggccgggttg ggtgggtggc aagcagacat ccacgtcgaa
     2581 gggcggtcca aaaggcgcta cttcccaaat aagaccgagg aaaatgcgag caagccgagc
     2641 atcccgatca gcgctgcgag gaatgtcgcc tgcgaaggcg ggaggttgtt atatccatct
     2701 ttgaccggat attgcgctct aagcgcgtta atatgcgccc gatggtccac ggcggccagc
     2761 aacagtgcca gcaatccgat gacaatcatg ataagcccaa actcctccgg cccgacgagg
     2821 cccttgtcct gaaaggttgc gccctttgtg atccgaaaaa actgttggat gctgaagccg
     2881 aacgtgatca gagacgtcgc agtcctgacc caagacagca tggttcgctc atgggataaa
     2941 taggtgcgag cgagcgagag cctgctaggg gtatcgggcg gtgcttttgt gctctcttca
     3001 tggtgctccg tcacaaaagc agccccttat ccgcggaatc gacgaagttg agcgcgatga
     3061 gaattgcgcc taacaataaa gaaacactcg aagccttggt agtccggaac
//
LOCUS       DATMGX010001046        12834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_141449, whole genome shotgun sequence.
ACCESSION   DATMGX010001046 DATMGX010000000
VERSION     DATMGX010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141449"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VNR65_17175"
     CDS             <1..298
                     /locus_tag="VNR65_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20452.1"
                     /translation="GHAVQEAAANIPAEVCARFDTAETLSDEDRATIVEIARNALARF
                     QPKPESKPKAKPQSKPEAKAEPTPEPESKPEAQTEVKPKPKPTPKDKPTPKEKS"
     gene            295..1188
                     /locus_tag="VNR65_17180"
     CDS             295..1188
                     /locus_tag="VNR65_17180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HWJ20453.1"
                     /translation="MSETTASLRRKINSAGDLQSVVRTMKALAASNIGQYERAVRALG
                     DYYRSVELGLSICFRKSAPAALVPEAKGRTDEGSVGAVVFGSDQGLVGRFNDVLADYA
                     VKMLAALPGKPRVWAVGERVHARLADAGLPLMGLFAVPNSVKAIIPLVGQILVESETR
                     QTLGEVTELHLFYNRPTSGPVYVPVSQRLLPLDENWRRKLAKLPWPTGSLPEVMGGAT
                     ATLRALIREYLFISLFRACAESLSSENASRLAAMQRADKNIDELLEDLNGTFHRLRQS
                     GIDEELFDVISGFEALSVKEK"
     gene            1468..2337
                     /locus_tag="VNR65_17185"
     CDS             1468..2337
                     /locus_tag="VNR65_17185"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HWJ20454.1"
                     /translation="MKIKSKDFRVREGDKVNLKKWPTTVKPAYKSKEQYQKLLEEHVA
                     QLSSQQQLLYASNRHAVLLIFQAMDAAGKDGAIRHVMSGINPQGCQVFSFKHPSPAEL
                     EHDFLWRTSRDLPERGRIGIFNRSYYEEVLIVRVHPEILRSEAIPDTPHHDKKVWRDR
                     YRSIVDLEKHLHGNGIRIIKFFLHLSKEEQRKRFLQRIDVPEKNWKFSLADVEERKFW
                     KQYMKAYEECLSATSTREAPWYVVPADDKENARLIVSQIILDTFEGLKMTYPKTDAKR
                     RKELQLIRERLAK"
     gene            2525..3919
                     /locus_tag="VNR65_17190"
     CDS             2525..3919
                     /locus_tag="VNR65_17190"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HWJ20455.1"
                     /translation="MERIENRTFDEIEPGDTANLVRTLTYKDIEVFAILSGDVNPTHV
                     DESFAKSDTFHNVVAHGMWSGTLISAVLGTELPGPGTIYVDQSLHFHRPVRLGDTITV
                     TVKVTNKIEETHRVIFDCRASNQRGEEVITGTADVIAPTEKISRPRVVLPEIELRQKG
                     RRYERLIEMTRGLDPIRTAVVHPVDTLSLLGAVEAARAKLIVPVLVGPEAKIRAAAAQ
                     AQLDLAPYEIVPTEHSDAAAVQAVAMARAGKVEALMKGALHTDELMHAVVDVDRGLRT
                     ARRVSHVFAIDAPGYPRPLFITDAAINIYPTLTDKRDIIQNAIDLVHALGIAEPRVAI
                     LSAIETVTEKIKSTLDAAALCKMADRGQITGGILDGPLAFDNAVSEEAAKTKGIISPV
                     AGRADIFVVPDLEAGNMLAKQLEYLAEAQIAGIVLGASVPIILTSRADKTLARLGSCA
                     IALLLVRHKAGVKP"
     gene            3916..5121
                     /locus_tag="VNR65_17195"
     CDS             3916..5121
                     /locus_tag="VNR65_17195"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HWJ20456.1"
                     /translation="MSDAILVLNAGSSSIKFSVFPGHLRPSRQGLICDGECEGIGHRV
                     HFKAKDSAGVSLVDEYLAEETTHEDALAALLCWLEGHFQGHRLVAAGHRVVHGGSLYT
                     APVRIDASVIVELRRLIPLAPLHQPHNLAAIAALSKLHPVLPQIACFDTAFHHTQPEV
                     AAAFALPRRLTTEGIRRYGFHGLSYEYIASVLPDILGPAEADGRVVVAHLGSGASMCA
                     MHRRKSVTTTMGFTALDGLPMGSRCGNLDPGVVLYLIQEKGMTAQAVSDLLYHSSGLF
                     GVSGVSDDMRTLLASDDPHAATAVALFVYRISRELGSLAAALGGLDALVFTAGIGEHA
                     PEIRRRVCEQSVWLGVHFDEAANAVGGPKITIADSRASAWVIPTDEDLMIARHTWRLT
                     KNDGMVRNR"
     gene            5379..6494
                     /locus_tag="VNR65_17200"
     CDS             5379..6494
                     /locus_tag="VNR65_17200"
                     /inference="COORDINATES: protein motif:HMM:NF022783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWJ20457.1"
                     /translation="MSKKLSVKSADLSLMSVPAFWPMAMAAKLVEEGAELYAKNLKFA
                     EEEIKIHDDLRPTLATPNQVRLDLRTMVLRDYGKPGGLPTLVDAPHAGHTAMIADYHK
                     GQSLVETLLANGIGHVALTDWKSATEDMKDLEIDNYLAEVIIAIDDLGGRVNLVGLCQ
                     GGWVAAMVAARFPDKVNSLVLAGAPIDTDAGNGPIKRMVHESPASFYEELVALGGGLT
                     KGKYMLQGWKNMHPEQHYIQDHVDLYEHIDDPAYLAKEETFESWYENPIDLPGRWYLQ
                     VITQIFKENRLAKGKFIGLGRQLNLHDISCAAYLLAGADDDITTPEQVLDAAKYLGTP
                     KERIVQKTVPGGHIGLFMGARTLKEHWPPIARWIAGQ"
     gene            7098..8309
                     /locus_tag="VNR65_17205"
     CDS             7098..8309
                     /locus_tag="VNR65_17205"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWJ20458.1"
                     /translation="MADQSTHNAATSMSSASTTRRTLLKGFAAAGAAISMSSKVAWAD
                     GEIGYWAKDLPPEKLIDMYSTILRIRWFERTLADKQLDDRSFRNLIHLYAGQEAVATG
                     VCAALNNKGPFNEIDVIYGTHRPSGHAIAKGVNMGKLAAEYNFKATGLNRGYAGEMHV
                     ADKSVGYMGGDGIVGPAAVIAAGSAFAFRARGTKQVSVVFGGDGTYATPAFHSSLNNA
                     ALLKLPFIYVVENNLYHQFAHYSYSCPMKDIATAASMYRIPGIVVDGQDVTQVYNKMK
                     DAVERARAGEGPTLIEAKTYRYYAHWGAPGATVGQLGSFGYDPQAITSWRPERELRDW
                     LQRDPVDICRNTLISWGVLDQAKAAAIETAIKQEVKEAFEWEAKQPLCKPEDGLKSVF
                     IEGPVAARQFG"
     gene            8354..9325
                     /locus_tag="VNR65_17210"
     CDS             8354..9325
                     /locus_tag="VNR65_17210"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWJ20459.1"
                     /translation="MARKNYLYAIYEAVQHEMRQRKDMTWLFELTPAVASNQGTPVIN
                     LEKEFGRTRVNNTGIDEMWMAGATLGAGLVGTTAVTYIPYQGSQVCFEMIQNHAGKLH
                     HMSGGDSTMPVVFIIELSGQSPGFAGQHSDYEADSYYAHIPGVKVVIASTPYDAKGMM
                     ASAIQDPNPVVYLLPAGLRQLNDEVPDEQYTVPLDKAAVRTTGSDLTIVSSGAGMPGV
                     LEAAAQLQKVGMKVEAIDLRSLKPMDTATLVASVQKTKRLLTFDQSYYTLCPGAEVIA
                     RVAENVDGARYKRIAFPDAPPPASPEMFTWMRPNASHIVAAATKLVG"
     gene            9402..9743
                     /locus_tag="VNR65_17215"
     CDS             9402..9743
                     /locus_tag="VNR65_17215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HWJ20460.1"
                     /translation="MDTAEQLSAACPVCESTDTRLARVRSAFWHNDRLVVIEDVPALV
                     CNACGEQFYDDAAVLMIDRMRQAGFPSENAVRELRVPVFAFGRVGTTDVPSPEASSFT
                     APLASEVLIRS"
     gene            9782..10384
                     /locus_tag="VNR65_17220"
     CDS             9782..10384
                     /locus_tag="VNR65_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20461.1"
                     /translation="MRFNFLAAAVVAATFSACLVQPLAAQTAEQLTFIPAGGRTLLTN
                     IATSKPPADELKALLTGKRSRDDWSGYLKAHSQAVPPLQSQSDKQRLTLADYLSFHMP
                     LPANQVPADLTRTDWTRTLPKDGRDLALDNCQGCHIITVVVTQVRTKTAWLGTLSTPA
                     HAVIKMTPAERDELASYLVLNAGIPIEQVPEELRASGATY"
     gene            10463..11920
                     /locus_tag="VNR65_17225"
     CDS             10463..11920
                     /locus_tag="VNR65_17225"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HWJ20462.1"
                     /translation="MLFTSLDFFIFLPLALLAFAIAPVAQRWAVLLLASYVFYGYLHP
                     FNLVYLGAVTLVIFGCGWGLERARQRPLRVAVFVMGLVAVLGSLTAFKFYDFLAGEIE
                     HANLFANLKLPRLGITAPAGYSFYVFSAASYLIDIYVGRLSGKQNLGQVALFTAYFPK
                     IFAGPIERATNFLPQLVSGLRADPLRFALGLQLIGWGLFKKVVIADNLAPLVDHSFKI
                     VAFAPPLELLISTYFFAFQIYCDFSGYTDIALGISSLFGLELMENFRRPYLSRSTAEF
                     WAERWHISLGRWFRDYLYIPLGGSRAGALRRAINVMVVFLVSGLWHAGLGYGVGWTFL
                     VWGALNGAYQWAGLATRGLWRRIGEALPRVAASAWLRVLRTVITFHLILLSWVFFRAG
                     SMHDALLVLQKIGARLTDLPHLLTIYPFSAEQLTGFGLIAFLVAYEIVDERRPVFQRL
                     ADAPVALRWGAYYLGIFALLLFGRWQAKEFIYMQF"
     gene            11936..>12834
                     /locus_tag="VNR65_17230"
     CDS             11936..>12834
                     /locus_tag="VNR65_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20463.1"
                     /translation="MKQFGKSALLFVAIGLLLYAGVFVATERLLLRNGHSNPIFKIAT
                     AEARDYDWVILGASHAMPFDFADFNALMQRETGLRILNLAAQGTGPLYNRFVIEHFLR
                     AHRTRNLLYVVDSFAFTSRAWNEDRFADAKLIRGTPFAPALARLMGNYVRRDGVDPHA
                     LLDYLSGFSKINNRDRFRRDAWEGEAQFDRVYRSSATAVAQRIAYLYPDATAPDVIAR
                     YLRDFASLIEIARQHNIRVIAIKLPTLALFHNQLPNEAAFDLASTQVLTDHAVPLQDF
                     SAALDNPNFYFDTDHLNRAGVTES"
BASE COUNT         2631 a         3849 c         3774 g         2580 t
ORIGIN      
        1 cgggcacgcc gtgcaagaag cggcagcgaa catcccggcg gaggtgtgtg cgcgattcga
       61 taccgccgaa acattgagcg acgaagatcg agcaacgatc gtcgagattg cgcgcaatgc
      121 acttgcacgc tttcagccca agccggaatc taaacccaag gctaaacctc agtcgaaacc
      181 tgaagccaaa gccgagccca cgcctgaacc cgagtcgaag ccggaggccc aaacggaggt
      241 caaaccgaag cccaagccga cacccaaaga caagccgaca cccaaagaga agtcatgagc
      301 gaaaccacag cgagcctgcg gcgcaagatc aacagtgccg gcgatctgca atccgtcgtt
      361 cgcacgatga aagccctggc ggcctcaaac atcggccagt acgagagagc ggtgcgcgca
      421 ttgggcgatt actatcgcag cgtggagctg gggttgagca tttgtttccg aaaaagcgcg
      481 ccggcagcgc tcgtgccaga agctaaggga cgaaccgatg aggggtctgt tggcgcggtt
      541 gtgttcggtt cggaccaggg actggtgggc cggtttaacg acgtgctggc tgattatgcg
      601 gtgaagatgc tcgccgctct gcccggaaaa ccccgagtct gggccgtcgg tgagcgcgtt
      661 catgcgcgct tggcggatgc gggcctgccg ctgatgggcc tcttcgcggt gccgaattcc
      721 gtcaaggcca tcattcccct cgtcgggcaa attctcgtgg agagcgagac gcgccaaacc
      781 ctgggtgaag tcaccgaact ccacctcttc tacaaccgcc ccacgtccgg gccggtttat
      841 gtgcccgtca gccagcgact gctaccgctg gacgaaaact ggcgacgcaa gctggccaaa
      901 cttccctggc cgacgggaag cttgcccgag gttatgggtg gcgccaccgc gaccttgcgg
      961 gcgctcatcc gcgaatatct tttcatctcg ctgttccggg cgtgcgccga atccctctcg
     1021 agcgagaacg cgagccgact ggccgcgatg caacgcgccg acaaaaacat cgacgaattg
     1081 ctggaggatc tcaacgggac attccatcgg ttgcgccaga gtggaatcga tgaggaactg
     1141 ttcgacgtca tctccggctt cgaagcgctg agcgtaaagg agaaatgatg cgcatttcct
     1201 gacccgcacg tgcggcccat tctggttcgt acgccccgcg cgggttctat gggtagccgc
     1261 actcggcgcg caacatgtgg cgactcttat tgcggcttat gggttgctta tgacacccct
     1321 cggctggggg tgggcactgt tcgtatgggg ctatgccccg gcttggttcc tcgtgaatga
     1381 cagcagtgaa actgctcgcg catcggattt ttgattaagt caaaagcggt tctgtaaata
     1441 atggaacgcg acgccgaaag gatggaaatg aaaatcaaat caaaagattt tcgcgtgcgg
     1501 gaaggagaca aggtcaatct caagaagtgg ccaacgaccg tgaagcccgc gtacaagtcg
     1561 aaggagcagt atcaaaaact gctggaagag cacgttgcgc aacttagttc gcaacagcag
     1621 cttctctacg catccaaccg ccatgccgtc ctgctgatct ttcaggcgat ggacgcggcc
     1681 ggaaaagatg gcgccatcag gcacgtgatg tccgggatca atccgcaagg ctgccaggta
     1741 ttcagcttca aacatcccag tcccgccgaa ttggaacacg attttctctg gcgcaccagt
     1801 cgcgatctac cggaacgcgg gcggatcggg atctttaacc gatcctatta cgaggaagta
     1861 ctgatcgtcc gtgtgcatcc cgagattctc cgcagcgaag cgattccgga cacgccgcat
     1921 cacgacaaga aggtctggcg cgaccggtat cgttcgattg tggatttgga gaagcacctc
     1981 cacggcaacg gaatccgcat catcaagttt ttcctccacc tctcgaagga ggaacagcga
     2041 aagcgtttcc ttcaacgcat cgatgtgccg gaaaagaact ggaaattcag ccttgcggat
     2101 gtcgaggagc ggaaattctg gaagcagtat atgaaggctt atgaggaatg cctcagtgcg
     2161 acaagcaccc gggaggcgcc ttggtatgtc gttcccgccg acgacaagga aaatgccagg
     2221 ttgatcgtct cgcaaattat tctcgacaca ttcgagggac tcaaaatgac ttaccccaaa
     2281 acggacgcaa agcgccgaaa ggaattgcag ttgatccgcg agcggctggc gaaatgagtt
     2341 cttgcgcact ccaatcctgt cataaatgcg atcggagaac gataatgttc aaacttatcg
     2401 agggtctatc gtaaccttgc ggccttcctg gtcagcgacg gcgcagccgc actcaccggg
     2461 aacatcgaat atgccgacgc cggctatcac atcgtcggct aacaggcggg tttggaggtc
     2521 cggaatggag cgcatcgaaa acaggacgtt tgacgagatt gagccgggtg acacggcaaa
     2581 cctcgtccgg acgctcacct ataaggacat cgaggtattc gcgatcctgt cgggagacgt
     2641 caatcccacg catgtagacg agtccttcgc caagagtgac acgttccaca acgtggtggc
     2701 gcatggcatg tggagcggga cgctgatctc cgcggtactc ggaaccgaac tgcctggtcc
     2761 gggcacgatc tacgtcgatc agtcgctgca tttccatcga ccggtacgtc tcggggacac
     2821 catcaccgtg acagtgaagg tgaccaataa gatcgaagaa acccacagag tcatcttcga
     2881 ctgccgcgca tcgaaccagc gtggtgaaga ggtgatcacc ggcaccgcag acgttattgc
     2941 accgaccgaa aagatctcgc gcccgcgcgt cgttctcccc gaaatcgagc tacggcagaa
     3001 ggggcgacgc tatgaacgcc tgatcgagat gacgcgcggt ctcgatccaa tccgtaccgc
     3061 ggttgtccat ccggttgata cgctgtctct gctcggcgcc gtggaagcgg ctcgtgccaa
     3121 acttatcgtg ccggtgttgg ttggtccgga agccaaaatc cgcgccgctg ctgcgcaggc
     3181 tcaactcgat ctggcgcctt acgagatcgt tccgacagag cacagcgacg ccgcggcggt
     3241 gcaagccgtc gccatggccc gcgccggcaa ggtcgaagcg ctgatgaaag gagcgctcca
     3301 taccgacgag cttatgcatg cagtggttga cgtcgatcgc ggcctgcgca ctgcccggcg
     3361 ggtaagccac gtctttgcga ttgacgcgcc cggctacccg agaccgctct tcatcacgga
     3421 cgccgcgatc aacatctacc ccacgctgac ggataagcgc gacatcatcc agaacgctat
     3481 cgacctggta catgccctgg gcatcgcgga accgcgcgtg gcgattctct ctgcgatcga
     3541 gactgtcaca gagaagatca agtccaccct cgatgcggcc gcactttgta agatggccga
     3601 tcgtggccag atcaccggcg gcatcctcga cggtccgctt gccttcgaca atgcggtgtc
     3661 ggaggaggcg gccaagacga aaggtatcat ctcgccagtc gctggccgtg ctgacatctt
     3721 cgtggttccg gaccttgagg ccgggaacat gctggcaaag caactcgagt atcttgcgga
     3781 ggcgcagatc gccggcatcg tgcttggcgc gagcgtaccg atcattctga cgagccgcgc
     3841 cgacaagacg ctcgctaggc tcggctcctg tgcgattgcg ctgttgctgg tgcgccacaa
     3901 ggcgggggtg aagccgtgag tgatgctatc ctcgtcctga acgccggatc ttcgagcata
     3961 aaattctcag tctttccggg ccatttgcga ccgagccggc agggcctcat atgcgatggc
     4021 gagtgcgagg gcatcgggca ccgtgtgcat ttcaaagcga aagacagtgc cggagtgtcg
     4081 ctggtcgatg agtatctggc agaagagacc acccatgagg atgcgctcgc cgcgcttctg
     4141 tgctggttgg aagggcactt tcagggccat cgactggtcg cggccggaca tcgggtggtg
     4201 catggcggat cactctatac cgcgccggtt cgtatcgacg cgtcggtgat cgttgagctt
     4261 cgccgcctca ttccgctggc accgctccat caaccccata accttgccgc catcgctgcc
     4321 ctctcgaaac ttcaccccgt attgccgcag attgcgtgct tcgacaccgc cttccaccat
     4381 acacagcctg aggtcgccgc cgccttcgcg ctgccgcgtc ggctcacgac cgagggtatt
     4441 cggcggtacg ggtttcatgg cctatcctac gaatacatcg ccagcgtcct tccggacatc
     4501 ctcggtccgg cagaagcgga tggtcgcgtg gtggtcgccc atctcggcag cggcgccagc
     4561 atgtgcgcga tgcaccggcg caagagcgtg acaacgacca tgggcttcac cgcactcgat
     4621 ggcctgccaa tgggcagccg ttgcggcaac ctcgatccgg gcgtcgttct ctacctgatc
     4681 caggaaaaag gtatgacggc gcaggcggta agcgacttgc tgtatcattc ctcaggattg
     4741 ttcggcgttt ccggtgttag cgatgacatg cggaccctgt tggcgagcga cgacccgcac
     4801 gcggccactg ccgttgccct gtttgtctac cggatcagtc gagagttggg gtcgctcgcc
     4861 gccgcgctcg gcggactgga tgcgctggtc ttcaccgcgg gcatcggtga gcacgccccc
     4921 gagattaggc ggcgagtgtg tgagcaatcc gtatggctcg gtgtgcactt cgatgaagcg
     4981 gcgaacgccg tcggcggccc caaaatcacg atagccgaca gcagagcctc cgcgtgggtc
     5041 attccgaccg acgaagatct gatgattgcg cgccatacat ggcgcctgac taagaacgat
     5101 gggatggtaa ggaatcgata acgcgatatg agatccggac acagagaagc gagaaccaca
     5161 tgagacgaca aaagacagca acgtccacgt tggacggagc gccggagatc aggccgcttg
     5221 gtgcagaaac gatcaagcga tgacacttgc gttcatgtca ccgccttccg accctgccgg
     5281 cctcattgtc atctaaatca atgtccgaaa gaaaatgacg ctttcgacat tcgcaaggtc
     5341 tgttttcgtt cattccgcta tgagcacaaa gatcagtcat gtcaaaaaag ttatccgtta
     5401 agtccgccga tctgtcgtta atgagcgtgc cggcgttctg gccgatggcg atggcagcca
     5461 agttggtcga ggaaggggcg gagctttacg cgaagaacct caaatttgct gaggaagaaa
     5521 tcaaaattca tgacgatctg agacccaccc tcgcgacgcc gaaccaagta cgccttgatc
     5581 tgcggaccat ggtgctgcgc gattatggca agccgggcgg acttcccacg ctcgtcgatg
     5641 cgccccatgc cggtcacacc gcgatgatcg ccgactatca taaaggccag agcctggtcg
     5701 agactctgct ggccaatgga attggccacg ttgccttgac cgactggaag tcggcgaccg
     5761 aggacatgaa ggatcttgag atcgacaact accttgccga agtgattatt gcgatcgatg
     5821 acctcggtgg acgcgtgaat ctggtcggcc tttgccaggg cggttgggtg gcggccatgg
     5881 tggcggcgcg attccccgac aaagtaaatt cactggtgct cgcgggcgca cccatcgaca
     5941 ccgatgccgg caacgggcct atcaaacgca tggttcacga atcccctgca tcgttctacg
     6001 aggaattggt ggctctcggc ggtggtctga cgaagggtaa gtacatgttg caaggctgga
     6061 aaaacatgca ccctgagcag cactatattc aggaccatgt cgatctctac gagcacatcg
     6121 acgacccggc ttatctggcc aaggaggaga cgttcgagag ctggtacgaa aatccgattg
     6181 atctgccggg acgctggtat cttcaggtga tcacccaaat cttcaaggag aaccggcttg
     6241 ccaagggcaa attcatcggg ctgggccgac agcttaatct ccacgatatt tcttgcgccg
     6301 cctatttact ggcgggtgca gacgatgaca tcacgacgcc ggagcaagtc cttgatgccg
     6361 ccaaatatct tggaacgcca aaggaacgaa ttgtgcagaa gaccgtaccg ggcggtcata
     6421 tcggactgtt catgggagct cgaaccttga aggagcattg gccgccgatt gctcggtgga
     6481 ttgccggcca atagatctta catcctgtct ataaacgcca aggtatgatc ccgcctgcag
     6541 gatggcggag atcccacccg accacaacag tcatgaaggc cgcacatagc ggctgtcgcg
     6601 ccgtctgcca cgtacagaaa atgtcctaga tttcctaaag gcgaatgggt gccggccagc
     6661 ttctatatct tcggttaccc tgtcgcatcg cccaatcaag aaatcgaagc tctggctgag
     6721 caccacctcc cggtcgtaac cggctagcga caggaagctg tcattggcaa aaatgagcgg
     6781 gtggttggat gctttggcat ccgcgaacaa cgttttttcg tttgacttcg gattatggga
     6841 gttattcgcg tgtgtccgat ggtacctcct ggataattct gcatcctcaa ttggctcatt
     6901 gccattgcag cgagtgccgc gacgatttgc cgcacgtcaa catgaagcac gccaggcttg
     6961 gctacgagcg cgatgcaccc gatgcccatg acggtttgat gaaccgcgtt tgcgctacat
     7021 caatgcgctt ttctgggaat tgagtccaaa gtttcaaatc ttggaaaagc tggctgacag
     7081 agctgacgga gacgaaaatg gcggaccaat ccacccacaa tgcagcaaca tctatgtcgt
     7141 ccgcgtcgac gacgcggcgc acgcttctga agggattcgc cgctgcaggc gcggcaatat
     7201 cgatgagtag caaggtcgct tgggccgatg gtgaaatcgg ctactgggcg aaagatttgc
     7261 cgcccgaaaa attgatcgac atgtattcga ccatcctgcg cattcgatgg ttcgaacgca
     7321 ctctggccga caagcagctg gacgaccgca gcttccgcaa tctgatacat ctctacgccg
     7381 gccaggaagc agtcgctacg ggcgtctgcg ccgcgctcaa caacaaaggg cctttcaacg
     7441 agatcgacgt catctacggc acacatcgtc cgagcggaca tgcgatcgcg aaaggcgtca
     7501 atatggggaa actcgccgcc gaatacaatt tcaaggcgac cggtctcaac cgcggatacg
     7561 cgggcgagat gcacgtcgct gacaagtcgg tcggctacat gggcggtgac ggaatcgtcg
     7621 gcccagccgc ggtgattgcc gccggttcgg cgttcgcatt ccgcgccaga ggcacgaaac
     7681 aggtctcggt cgttttcggc ggcgatggca cttatgcgac gcccgccttc catagctcat
     7741 tgaacaacgc cgcgctgctc aaactgccct tcatctacgt ggtggagaac aatctctacc
     7801 accagttcgc gcactactcc tattcctgcc cgatgaaaga catcgcgacc gccgcaagca
     7861 tgtaccgaat ccccggcatc gtggtggacg gccaggacgt gacgcaggtc tacaacaaga
     7921 tgaaggatgc tgtggagcgt gcgcgggccg gcgaggggcc gaccctgatc gaagcaaaga
     7981 cctaccgcta ctacgcccac tggggcgctc ccggagccac agtcggtcaa ctcggctcct
     8041 tcggctacga tccgcaagca atcacctcgt ggcgtccgga acgggagttg cgggattggc
     8101 tgcagagaga cccggtcgat atctgccgca acactctgat ctcgtggggt gtgctcgatc
     8161 aggccaaggc tgccgcgatc gaaaccgcca taaagcaaga ggtcaaggaa gccttcgagt
     8221 gggaggccaa gcaaccgctg tgcaagccgg aagacggact gaaaagcgtc ttcattgaag
     8281 gccctgtcgc tgcgcgccag ttcggctgag caaagggctg atcgcaaccc cattcgaaaa
     8341 tcggagaagc acaatggcac gcaagaatta tttgtacgcg atttacgagg ccgtgcagca
     8401 cgagatgcgc cagcgcaaag acatgacctg gctcttcgag ctgacgcccg cggtcgcttc
     8461 gaaccaagga acgccagtca tcaacctcga gaaggagttt ggccgcaccc gcgtcaacaa
     8521 caccggtatc gacgagatgt ggatggccgg tgccacgctt ggcgcggggt tggttggcac
     8581 caccgccgtg acctacatcc cctatcaggg aagtcaggtt tgcttcgaga tgattcagaa
     8641 ccacgccggc aaattgcacc acatgagcgg tggcgactcc acgatgccgg tggtgttcat
     8701 catagaattg tcgggccaga gtcctgggtt tgccgggcag cattccgact atgaggccga
     8761 cagctactat gcccacatcc ccggggttaa ggtcgtgatt gcatcgacgc cctatgacgc
     8821 caagggcatg atggcgtcgg caatccaaga tccaaacccg gtcgtctatc tcttgccagc
     8881 gggcttgcgg cagttgaacg acgaggttcc cgatgagcag tacaccgtac cgctcgacaa
     8941 ggcggccgtg cgcacaaccg gcagcgatct caccatcgtg agctccggcg ccggtatgcc
     9001 gggagtgctt gaggccgctg ctcaactgca gaaggtcggg atgaaggtcg aagcgattga
     9061 cctgcgctcg ctcaagccga tggacacggc gaccctggtt gcctcggtgc agaagaccaa
     9121 gcgccttctc acgttcgacc agtcctacta cacgctctgc ccgggcgccg aagtcattgc
     9181 gcgcgtggcc gagaacgtgg acggcgcgcg ctacaagcgg atcgcatttc cggatgcgcc
     9241 gccgcccgca tctcccgaaa tgttcacctg gatgcgcccg aacgcgagcc acattgtggc
     9301 ggccgcgacg aagctggtgg gctgaagctt ctcattcgac cgcgggccga aggcggggtt
     9361 ctgccttcgg accgcggtcg aaccaaaaag ttggtacaaa aatggacacc gctgaacagc
     9421 tatccgccgc ctgtcctgtt tgcgagagca cggacacccg tctggctcgc gtgcgctcag
     9481 cgttttggca caatgatcgc ttggtcgtga tcgaggatgt gcccgcgctt gtgtgcaacg
     9541 cctgcggcga gcagttctat gacgatgctg ccgttctcat gatcgatcgg atgcgccagg
     9601 ccggctttcc gtctgaaaat gctgtccgcg aactgcgtgt accggtattc gcttttggca
     9661 gggtcgggac aacggatgtc ccgtcaccgg aagcgtcgtc tttcaccgct ccgctcgcga
     9721 gcgaggttct gatccggtcc tgaagccggc tcgttcacca gtcacgagaa ggatatcaat
     9781 catgcgcttc aattttttag ccgccgccgt cgttgctgcg acgttcagcg cttgccttgt
     9841 tcagccgctc gccgcgcaga ctgctgagca gttgactttc atccccgcgg gcggccgaac
     9901 gctcttgacg aacattgcta ccagcaagcc gccagccgac gagctcaaag ctttgctcac
     9961 aggcaagcgc tcgcgcgacg actggtcggg ctatctcaag gcccacagcc aagccgttcc
    10021 cccgttacag agccagagcg ataagcaacg gctcacgctt gccgactatc tctccttcca
    10081 catgccgttg ccggccaatc aagtcccggc agacctgaca cgcaccgatt ggacgcgtac
    10141 tctgccaaag gacgggcgcg atttggcgct cgacaattgc cagggctgcc acatcatcac
    10201 cgtcgtcgtc acccaagtgc ggaccaaaac ggcttggctc gggaccctca gcacgccggc
    10261 tcatgcggtc attaagatga cgcccgcgga gcgtgacgaa cttgcgagct atctcgtgct
    10321 gaatgcgggc atcccgatcg aacaagttcc ggaggaattg cgggcgtccg gcgcaactta
    10381 ttgacgcggg ctttggcgcg cacgcgagga atggcgaacg acgcgtaagg cgacgtgatt
    10441 ctgcggatcg agatccaagg tcatgctgtt cacgtcgctc gatttcttca tcttcttgcc
    10501 attggcgctg ctggcattcg cgatcgcgcc ggtcgcgcaa cgctgggcgg tattgctgct
    10561 cgccagctac gtgttttatg gatatctgca ccctttcaat ctggtctatc tcggcgcggt
    10621 gacgctggtc atatttggct gcgggtgggg tcttgagcgg gcaaggcagc ggcccctgcg
    10681 cgtcgctgtt tttgtcatgg ggcttgttgc ggtgctcggc tcgctcaccg ccttcaaatt
    10741 ctacgatttt cttgccggcg agatcgagca cgccaatctg ttcgcaaatc ttaagttgcc
    10801 gcggctcggc attactgccc cggccggcta ctcattttat gtcttctcgg ccgcgagcta
    10861 tctcatcgac atctatgtcg ggcgtctttc gggcaagcag aatctcggcc aggtggcatt
    10921 gttcaccgcc tattttccga aaattttcgc cggcccgatc gaacgagcga cgaatttcct
    10981 gccgcagctt gtgtccgggc tgcgcgccga cccgctccgt ttcgcgctcg ggctgcagct
    11041 catcggctgg ggactgttca agaaggtcgt cattgccgac aaccttgccc cgctggtcga
    11101 tcacagcttc aagatcgtgg ccttcgcccc gccgctcgaa cttctgatca gcacgtattt
    11161 cttcgcgttt cagatctatt gcgacttttc cggctacacc gatatcgcgc tcggcatctc
    11221 ctcgctgttc ggcctcgagc tgatggagaa cttccgccgg ccgtacctgt cgcgcagcac
    11281 ggcggaattc tgggcggagc gctggcacat ttcgctcggc cgctggttcc gcgactatct
    11341 ctatatcccg ctgggcggca gccgcgccgg tgctctgcgc cgcgccatca atgtcatggt
    11401 cgtcttcctg gtgagcggcc tatggcatgc cgggctcggc tacggagtcg gctggacctt
    11461 tctcgtctgg ggcgcactca acggcgccta tcagtgggcg gggctggcga cgcgcggcct
    11521 gtggcgccgc atcggcgaag ccttgccgcg cgtggccgcc agcgcctggc tgcgcgtttt
    11581 gcgaaccgtc atcaccttcc atctgatcct gctgagctgg gtattcttcc gcgccggatc
    11641 gatgcatgat gcgttactcg tcttgcagaa gatcggcgct cggctcaccg acctgccgca
    11701 tctgctgacc atctatccgt tcagcgccga gcagctcacc ggatttggcc tgatcgcctt
    11761 cctggtcgcc tatgaaatcg tcgacgagcg ccgcccggtg ttccagcgct tggccgatgc
    11821 gccggtcgcg ctgcgctggg gcgcctatta tctcggcatc ttcgccctgc tgctgttcgg
    11881 ccgctggcag gcgaaggaat tcatctacat gcagttctga catcgacatg cgaccatgaa
    11941 acagttcggc aaatcggcac tgctgttcgt cgcgatcggc ctgctgctct acgcgggcgt
    12001 gtttgtcgcg accgagcgat tgctgttgcg caatgggcac agcaatccga tcttcaagat
    12061 cgccaccgcc gaggcgcgtg attacgactg ggtgatcctc ggtgcctcgc acgccatgcc
    12121 gttcgatttc gctgatttca acgcactgat gcagcgggag accggcttgc gcatcctcaa
    12181 tctcgcggcg cagggcaccg gtccgctcta taaccgcttc gtgatcgagc atttcctgcg
    12241 cgcgcatcgc acgcgcaatt tgctctacgt ggtcgattcg ttcgccttta cctcgcgcgc
    12301 ctggaacgag gaccgcttcg ccgatgcgaa actcattcgc ggcacgccgt tcgcgccggc
    12361 gctcgcgcgg ctcatgggga attatgtgcg ccgcgatggc gtcgatccgc atgcgctgct
    12421 tgattatctc agcggctttt ccaagatcaa caaccgcgac cgcttccggc gcgatgcctg
    12481 ggaaggtgaa gcgcagttcg atcgggtcta tcgctcgtcg gcgacggcgg tcgcccagcg
    12541 catcgcatac ctctatccag acgctacggc gccggacgtg atcgcacgct atctgcgaga
    12601 cttcgccagt ctcattgaga tcgctcgaca gcacaatatc cgcgtcattg ccatcaaact
    12661 gccgacgctg gcgctgttcc acaatcagct tccgaacgag gctgcgttcg acctcgcctc
    12721 aacgcaggtg ctcactgatc atgcggttcc gttgcaggat ttctccgctg ctctggacaa
    12781 cccgaatttc tacttcgaca ccgaccattt gaaccgcgcc ggggttaccg agtc
//
LOCUS       DATMGX010001047         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_241332, whole genome shotgun sequence.
ACCESSION   DATMGX010001047 DATMGX010000000
VERSION     DATMGX010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241332"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..510)
                     /locus_tag="VNR65_17235"
                     /pseudo
     CDS             complement(214..510)
                     /locus_tag="VNR65_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(695..>2661)
                     /locus_tag="VNR65_17240"
     CDS             complement(695..>2661)
                     /locus_tag="VNR65_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWJ20464.1"
                     /translation="AVNDAAVVRYGYSREQFLTMNVTDIRTGDEESSAAFLRMIPDSQ
                     NEEYIGQHKRADGTKMHVSVYSRTLNYENREARLVALNDITDRKLAEDDLRRTKMFLD
                     AVIENVPMPIVVKTANDSRFTLLNRAGEELFGFNRDQVIGKTPHDIYDKEHADRIVAQ
                     DKDSLLSGKTLYIPDVTVTTPERGARLITSKKVAIQGSDGKPEYLLTLIDDVTERRQA
                     EQRITHMAHSDSLTDLPNRAAFNECLASTLRLAEAEGRSFALLCMDLDGFKKVNDVYG
                     HTIGDSLLRHVADRLRTVADDAFIARLGGDEFTIIADIRDRSSITELADRMLAAFGDD
                     FEVDGRRLRQELSIGAAIYPADGTDAETLLNHADAALYGAKAEDRGSVKFFEAKMGAR
                     LRERTALQNDLRSAIQNNELRLHYQPQLKMTGEITGFEALARWQSPKRGMVPPGEFIP
                     IAEESSLILLIGEWVLRETCREAASWKKPLRVAVNISPIQFRHGDLPRLVHSVLLETG
                     LAPGRLELEITEGVLIDDFSRAVSILRRLKSLGVQIALDDFGTGYSSLSYLHAFPFDK
                     IKIDRTFISGLENSRHSIAIVRAVIGLSRTLNIPILAEGVETQAQHAFLAQEGCDEVQ
                     GYLTGRPLAIADYAGVVGRKEIVTTYAHAV"
BASE COUNT          582 a          744 c          729 g          606 t
ORIGIN      
        1 acagttcgca gcatggaata ctcgcagttc ctgcattcga tcgtttgcct ttcatgacct
       61 gggcgtaccg tatgcggcgt ccggcgcgat acgtacatcg cgcccccaca tttaaggcac
      121 ttgatctcat catcggcacc aaacggctta gccatgcgcg ttagaacacg tagcgccacg
      181 cgagtagaat gatcaggatc aggactacga ctgttggtgc acgcgtacga tggacgtatc
      241 aatcatctgg acggccacgt tatgaaaggc ggtcagggca tccataatct ggtcccagac
      301 gccagccctg cgccaccgca cgaagcggtt gtagcaggtc gtgtaaggac cgaagctctc
      361 cggcagatcg cgccacggcg cacctgaacg caagatccaa aagatgccgt tgagaatgcg
      421 gcggtcgtct acacgcggca cgccgcgcgg tttgtttgga agcatcggat tgatgacgcc
      481 ccactcatag ttgctgagct cataccgcat gattcgagtc cccagtttgg ggagcttgaa
      541 tcatggccag tgcgacgcaa tcaacggtga attagcatgt ccaaaattgc cggctaaccg
      601 gcaaatggcg gacattgagg aatcaacgcg aatgaccatt tgcagccaca gactgtgact
      661 ttagaacaaa tttgaaggtc gggctgccta gctatcaaac cgcgtgagca taggtcgtca
      721 caatctcttt gcgtccgaca actccggcgt agtccgcgat cgcaagcggg cggcccgtca
      781 aatagccttg cacctcatcg cagccttctt gcgcgagaaa tgcgtgttgc gcctgcgtct
      841 cgacgccctc agccaggatg ggaatattaa gggtccggct gaggccgatc accgcgcgaa
      901 caatcgcgat cgagtggcgg ctgttttcca ggccggaaat aaatgttcgg tcgatcttta
      961 tcttgtcgaa ggggaacgca tgcagatacg acaatgatga gtatcccgtt ccgaagtcgt
     1021 ccaacgcaat ctgtacgccg agcgatttaa gcctacgcag aatcgacacg gcacgcgaaa
     1081 aatcgtcgat taatactccc tcagtgattt caagttcgag ccggcctggt gcaagtccgg
     1141 tttcaagcag aacggaatga accagcctcg ggaggtcgcc gtgacggaac tggatgggag
     1201 agatgttgac ggcgactctc aagggttttt tccacgaggc ggcttcgcgg cacgtctcgc
     1261 gcaacaccca ttcgccgatc aggaggatca ggctgctttc ttcagcgatg ggaatgaact
     1321 cgccgggcgg caccattccc cgcttcgggg attgccagcg cgccaaggct tcaaatcctg
     1381 ttatttcgcc ggtcattttc agctgcggtt gatagtgcag ccggagttca ttgttctgga
     1441 tcgcagatct taaatcgttc tgcaatgccg tccgctcgcg caatcgtgcg cccattttcg
     1501 cctcgaaaaa cttgacggaa ccgcggtctt cggccttggc cccatagagc gcggcatccg
     1561 cgtggttcag cagtgtttcg gcatcggtgc catcggcagg gtaaatcgcg gcgccaatgc
     1621 ttagttcttg tcgcaggcga cggccgtcaa cttcaaaatc atcgccaaaa gccgcgagca
     1681 tacggtcggc gagctcggtg atgctcgatc ggtccctgat atcggcaatg atcgtaaatt
     1741 cgtcgcctcc tagccgcgcg atgaaggcgt cgtcggcgac cgtacgcaag cggtctgcaa
     1801 cgtggcgcag cagcgaatcg ccgatggtat gcccgtaaac atcgttgacc ttcttaaatc
     1861 catcgaggtc catgcacaga agtgcgaaac ttctgccctc agcctccgcc agtctcaacg
     1921 tcgaggccaa gcactcatta aaggcggctc gatttggaag atccgtaagc gaatcgctat
     1981 gcgccatatg cgttatacgt tgctccgcct gcctacgctc cgtcacatca tcgatcaagg
     2041 tgagaagata ttccggcttt ccatcgctcc cttgaatggc gaccttcttc gaggtgataa
     2101 ggcgagcgcc ccgttcgggc gtggtaacgg taacgtccgg aatgtagagg gttttcccgg
     2161 aaagcaaaga atccttgtcc tgagctacga tacggtcggc gtgttccttg tcgtatatgt
     2221 cgtgcggcgt tttaccgata acctgatcgc ggttaaatcc gaaaagctct tcgcccgccc
     2281 tgttgagcaa tgtaaagcga ctgtcgttgg cggtcttaac cacaatcggc atcggtacgt
     2341 tttcgatcac ggcatcgaga aacattttcg tgcggcgaag atcatcttcg gccagcttgc
     2401 ggtcggtaat atcgttgagc gccaccagtc gagcttctcg attttcataa tttaaagttc
     2461 ttgagtagac cgagacatgc attttcgttc cgtcggcccg tttgtgttgc ccgatgtatt
     2521 cctcattttg agaatccggt atcatacgaa gaaacgctgc ggagctctct tcatccccgg
     2581 tccgaatgtc ggtgacgttc atcgtcagaa attgttcgcg gctgtatccg taacgcacga
     2641 cagcggcgtc attgaccgcc a
//
LOCUS       DATMGX010001048         2021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_267258, whole genome shotgun sequence.
ACCESSION   DATMGX010001048 DATMGX010000000
VERSION     DATMGX010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267258"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            535..1272
                     /locus_tag="VNR65_17245"
     CDS             535..1272
                     /locus_tag="VNR65_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWJ20465.1"
                     /translation="MRDWTAEELKSIAERLRRARITAGFERASDAARRFGWSYSRYMN
                     YENGERSVPPKQAILFASAYGVTVDYIYFGKRESFNQIEGTQTLISRMVRRIPLVALE
                     NMSELKRIASGLEPTSVTVPVGSLESIPERGIFIEIKDKSMSNSREPISFEPGDKVMI
                     DLDANPTPSDYVLALVPEEQTALFRLYREVGRADDGSIIVDLVPLNPNFRTVRISNAS
                     QGQIMGVCRSIHRIFDLTPAAQRARFG"
     gene            1520..>2021
                     /locus_tag="VNR65_17250"
     CDS             1520..>2021
                     /locus_tag="VNR65_17250"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWJ20466.1"
                     /translation="MLDASMARHLSHKPQSRQAALTLGAIGVVLGNIGTSPLYAVDQI
                     FYGPAHIAPTPDNVLGCISLVIWALTLIVSIKYAIFILRADADGEGGVFALYSLLHKY
                     KEETVYLPVLLAGLMLGAGFLFGDGIISPAISVLAAVEGLEVATPMFKSAVAPITVVI
                     LFLLFAV"
BASE COUNT          416 a          573 c          555 g          477 t
ORIGIN      
        1 tcgcgccgtt aatccttatg ccctcgtcgc cagaacgctt gcgactgccc gcgcgaagct
       61 atcgccagcg ctgcgtcatc ggaataacgg cgtaaatgca atcacttgcc tcccggttcg
      121 gcctaatacg ccgagcctga agacagcttt acgaaccgtt cgtgtcgctc gttctagtcg
      181 cgcgggacgc tcaatgattt ctgttattta actttgtgga gcagccccta tggcctatag
      241 ctacacacat acggttttaa aacacacaga ttcggactcg tttttccgga agctgggcga
      301 gattacacgt atcgtgtaac ccatgcttga atcgattttt tccgcctcgc cgcgtcaatc
      361 gctaagacgt tggccttgcg caggaataaa gaagtaactt gcgcgttagt tagaatgccc
      421 aaagctgcgc accaagcccg tgcgcccctt tcgatgcatt tgtgatattg ttcaacacag
      481 tttcagaagt tatgttcgga gtgctacacg gaatgtgggg acgggacggg ggaaatgaga
      541 gactggacgg ctgaagagct caaatcgata gctgaacgtc ttcggcgcgc tcgcatcact
      601 gcaggcttcg agcgcgcgag cgacgcagca cgcaggttcg gctggagtta cagccgctac
      661 atgaattacg aaaacggcga gcggtccgtc ccccccaagc aggctatcct gttcgcttca
      721 gcctacggcg ttaccgtcga ttatatttat tttggaaaaa gagagagttt caatcaaata
      781 gaagggacac agacgttaat ttcgcgcatg gtccggcgta tacctcttgt ggcgcttgag
      841 aacatgtcag aattaaaacg tattgcatcg ggcctggagc ccacctctgt caccgtccca
      901 gtcggcagcc tcgagtcgat cccggagagg ggaatattca tcgagatcaa ggacaagtcg
      961 atgtccaact ctcgggagcc catctccttc gagcccggcg ataaggtgat gatcgacctt
     1021 gacgcgaacc ctacgccatc cgattacgta ctggcgttgg taccggaaga gcagacggcc
     1081 ttgttccgtc tctaccggga agtgggtcgc gcggacgatg gctcgatcat cgtcgatctc
     1141 gttccgctca acccgaattt tcggaccgtt cgtatatcca atgcgagcca gggacagatc
     1201 atgggcgttt gccgcagcat ccaccgcatc ttcgacctga cgccagcggc gcaacgtgcg
     1261 cggttcggct agccggattt ccgcgcgcat tccatagtcc gccgtccgga actaaggatg
     1321 tattccatag cgtcactctg cgcttgaaaa gctctggcaa aaagatgggc cgtaagcgat
     1381 agtgcgtgga acacgttccg ctcgctgact ttttctgcaa ctttattcct ttttgagcag
     1441 gcggtgcagt gtcagggaca cgcccgatgc gccgggaatc cggtttaagc ttatgctcgt
     1501 cgggcgcgcg ggtgtgacca tgcttgacgc ttccatggcg cgtcatctgt ctcataagcc
     1561 gcagagccgg caggccgcgc tgacgcttgg cgcaatcggc gttgttttag gtaacatcgg
     1621 aacatcgcca ctttatgccg tcgaccagat attctacggc ccggcacata tagctccaac
     1681 gccggataac gttctcgggt gcatctcgct cgttatctgg gcgctcaccc tcattgtcag
     1741 tattaaatac gcgatcttca tcctgcgcgc ggacgccgac ggcgaagggg gtgtgtttgc
     1801 cctctacagc cttctgcaca aatataaaga agagacggtg tacctgcccg tcttgctggc
     1861 cggtctcatg ctcggcgccg gcttcctgtt cggggatggc atcatctcgc ccgcaatcag
     1921 cgtgctggcg gccgtcgagg gcctggaggt cgcgacgccg atgttcaaga gcgcggtcgc
     1981 ccctattacg gttgtcattc tctttctttt gttcgctgta c
//
LOCUS       DATMGX010001049         4091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_469366, whole genome shotgun sequence.
ACCESSION   DATMGX010001049 DATMGX010000000
VERSION     DATMGX010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469366"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2169
                     /locus_tag="VNR65_17255"
     CDS             <1..2169
                     /locus_tag="VNR65_17255"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HWJ20467.1"
                     /translation="PKVTSTVWKENVRIADENNQPGKFTAFCSYEWTSMPGQRNLHRN
                     VFFRSCDKVPDYPFSALDSKRPTDLWDWMDTQRKAGNELLAISHNANVSDGWMYPVDV
                     DNTTGRPIDAAWAASRDRNERLVEIKQGKGQSETHPLLSPTDEFANYELYQTILGQAA
                     DVGRIDHISGSFARQALKDGLAMQDLRGYNPYKFGMAGGSDSHNTGSSYRQDNFFGLH
                     ADADGSVERRFAGVLIGGTMDVRLENPGGLTGVWAEENTRASLWDAMYRKETFGVSGP
                     HIKVRFFGGWGFDKDIANARDWVKQSYAGGVPMGADLAPMPSGGKGTAPSFVVWALKD
                     PTSANLDRLQIIKGWTQNGQSFEKIYDVAWSGNRQPDKWTGRVPAIQSTVDIEKATYT
                     NSVGSTELKTVWTDPDFDASLHAFYYVRVLEIPTPRWTLIQAVKSGLPPPDVVPLTGQ
                     ERAWTSPIWYAPSAEARKNAETGTTVDDLKKKGAAILSDSELRDLIVGKAHWLRNKVT
                     GEQFWQSFTAEGQTTLFRIGSEATMPSGYGNVERGGYQGTTIPYKVEGGKLITFVSQD
                     PYAFTFFKLGDTYYAARSNEFGFANYEIIPPPKFAVNPLDEVINELSSTLGLTEQQRQ
                     QAFPIIKQEFMDLAALKKDTSLSAARKVERLREIGVSFDEKLKPLINAEQQEKFQAFR
                     KQFRRRLMEKLLRKAEEAVEADIKDVEADVWTWFTDKSEK"
     gene            2205..3311
                     /locus_tag="VNR65_17260"
     CDS             2205..3311
                     /locus_tag="VNR65_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20468.1"
                     /translation="MTRCFFAAIVLCTSSLATACPLCLGAFRSTAAQRLVELPHAVLA
                     QPSADGHDYRVLAVIKGDRPAAGTIPADAIELDFVADPAATLLLIRDDAWSIWVGVGP
                     VTVDHANWLRQIAEGKRSSEMNANEWQARVALMLPYLEHPEPLVAKIAYGELAAAPYA
                     ALLAAKPLLAADAIRRWLADPELSARRPLYLLLLGVAGNSRDAAVLEHQLEASWMAGD
                     ATNLSSMIAADLQLRGPSRMAWVDAKYMGDRRRSTRELEAALLALSVLGNANDAIPRE
                     RVVQSYLMFIHEHQELAGFVASDLAAWQYWDAAPLYLALIKSKVRQHFASQIAIFQYL
                     QQSSAANKIDAEVTRTGASNQTNGERPAFPVSPQ"
     gene            3308..>4091
                     /locus_tag="VNR65_17265"
     CDS             3308..>4091
                     /locus_tag="VNR65_17265"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWJ20469.1"
                     /translation="MTFTNHLYEPHWVSSVMKRFAREPLIHFLLLGAILFGAYAYVER
                     KQGGAEQSNEIKLTIGDISQLVLVFRSQWRRDPTPQELRQLVEDKVREEVLYREALAL
                     GLDRDDTIVKRRMAQKMQFLAEDVAAAREPTVEELGPWYEANHDQFAFPPRVSFRHLY
                     FSADRRGASARDDAATALTKLAGQPENSNFAATLADASMFQEYYRDRTPEFLGKEFGP
                     NFALTVVKLPPGSWQGPVESGFGWHLVFVDKLIPGRVPPFEEV"
BASE COUNT          848 a         1216 c         1265 g          762 t
ORIGIN      
        1 ccgaaggtca cttccaccgt gtggaaggaa aacgtaagga tcgccgacga aaacaatcaa
       61 ccgggcaagt tcaccgcatt ctgctcctac gagtggacgt cgatgccggg tcagcgcaac
      121 ctgcaccgca acgtcttttt ccggtcctgc gacaaggtac ctgactatcc cttcagcgcg
      181 ctggattcca agcgtcccac ggacctgtgg gactggatgg acactcagcg caaggcaggc
      241 aatgagctgc tggcgatctc gcacaacgca aatgtgagcg acggctggat gtaccccgtc
      301 gacgtcgaca acaccactgg aaggccaatc gacgccgcgt gggcagcctc gcgcgaccgc
      361 aacgaacgcc tggtcgagat caagcaaggc aaggggcagt cggaaacgca cccactgctg
      421 tcgcccaccg acgagtttgc gaactacgag ctttatcaaa ccatccttgg ccaagcggcg
      481 gacgtgggcc gcatcgatca tatctcaggc agcttcgcgc gtcaggcgtt gaaggacggc
      541 ctcgccatgc aggatttacg cggttacaac ccgtacaagt tcgggatggc aggcgggtcg
      601 gactcacaca ataccggcag ctcctatcgg caggacaatt tcttcggcct tcacgccgat
      661 gccgacggtt cggtcgagcg gcgttttgct ggcgtgctta tcggcggcac catggacgtt
      721 cgcctggaaa accccggcgg gctgaccggt gtgtgggcag aggagaacac gcgcgcttcc
      781 ctctgggacg ccatgtaccg caaggaaacc ttcggcgtca gcggcccgca catcaaggtg
      841 cgcttcttcg gcgggtgggg ctttgacaag gatatcgcga acgcccgcga ctgggtgaag
      901 caatcctacg cgggtggcgt gccaatgggc gcggacctgg cgcccatgcc atcgggcgga
      961 aagggcacgg ccccgagctt cgtcgtctgg gccctaaagg acccgacgtc ggccaatctc
     1021 gatcggcttc agatcatcaa aggctggacg cagaacggtc agagcttcga gaaaatctac
     1081 gacgtcgcct ggtccggcaa ccgccagccc gacaagtgga cgggccgggt gccggcgatc
     1141 cagagcaccg tggatatcga gaaggcgacc tacacgaaca gtgtcggctc gacggaactg
     1201 aagacggtat ggactgaccc ggatttcgat gcgagccttc acgcttttta ttatgtgcgc
     1261 gtattagaga ttccgacgcc gcgctggacg ctgatccagg ccgtgaaatc tggactgccc
     1321 ccgcctgacg tggtgccgct caccggccag gaacgcgcat ggacctcgcc gatctggtac
     1381 gcgccatctg cagaagctcg caagaatgcc gagacaggca cgacggttga cgaccttaag
     1441 aaaaaggggg ccgccattct aagcgactcg gagcttagag acctcatcgt ggggaaggcc
     1501 cattggctgc ggaacaaggt gacgggagag cagttctggc agagtttcac ggccgaggga
     1561 cagacgacgc tgttccgcat cggaagcgaa gcgaccatgc cgagcggtta cggcaacgtg
     1621 gagcgtggtg gataccaagg cacgaccatc ccctacaagg ttgagggcgg caagctcata
     1681 accttcgtgt cacaagaccc gtatgcgttc acgttcttta agttgggcga cacttactac
     1741 gccgcccgca gcaacgagtt cggcttcgcc aactacgaaa tcatcccgcc gccgaagttc
     1801 gcggtcaatc cactcgacga ggtgatcaac gaactctcga gcaccctcgg gctcaccgag
     1861 caacagcgtc agcaagcctt cccgatcatc aagcaggagt tcatggatct ggcggcgctg
     1921 aagaaggaca cctccttgag cgcggcgcgc aaggtagagc gtcttaggga gatcggcgtt
     1981 tcctttgacg agaagctgaa gccgctaatc aatgcagagc agcaggaaaa gtttcaggct
     2041 ttccgcaagc agtttcggag gcgcttgatg gagaaattgc tgcgcaaggc ggaggaggcc
     2101 gtagaagctg acatcaagga cgtcgaagca gacgtctgga cttggttcac ggacaagagt
     2161 gaaaagtagc gctcgatcag cgggaaagac gtggaatggg ggtcatgacg cgttgttttt
     2221 tcgccgcgat cgtgttgtgc acgagctcgc ttgcgacggc gtgcccgctt tgcctggggg
     2281 cttttcgatc aacggcggcg cagcggctgg tggaattgcc gcacgctgtg ctggcgcagc
     2341 cctccgctga tggacacgat taccgggtcc tggctgtgat aaagggagat cgaccggcgg
     2401 ctgggaccat cccagctgac gcgatcgagc ttgattttgt cgcagacccc gcggcgactc
     2461 tactcctcat tcgcgacgac gcgtggtcga tatgggtcgg cgtcggcccc gtcaccgtgg
     2521 accacgcaaa ctggcttcgt cagatcgcgg aggggaagcg ctcgtcggaa atgaatgcca
     2581 acgaatggca agctcgagtc gccttgatgc tcccctatct cgaacacccc gagcccttgg
     2641 tcgcgaagat agcttatggg gaacttgcgg ccgcgcccta tgccgcgctg cttgcggcga
     2701 aaccgctcct cgccgcagat gcgatcaggc gctggctcgc cgatccggag ctctcggcac
     2761 gacgtccact gtacctgctg ctgctcggtg tcgccggcaa ctcgcgggac gccgccgtcc
     2821 ttgagcacca gcttgaggcg tcctggatgg ctggcgacgc gacaaatctg agttcgatga
     2881 tcgccgccga tctgcagttg cgcggtccgt cgcgcatggc ctgggtcgac gcgaaataca
     2941 tgggcgatcg ccgccgctct actcgggagc tggaagcggc tctcttggcg ctcagcgtgc
     3001 tgggaaacgc gaacgacgca ataccacggg agcgtgtggt ccaatcttat cttatgttca
     3061 ttcacgagca ccaagagctt gccggttttg tcgcgtcgga tcttgcggct tggcaatatt
     3121 gggatgcggc gcccttatac ctcgcgctaa tcaaatcgaa ggttcggcaa catttcgcat
     3181 cgcaaatcgc aatcttccag tatctgcagc aaagttcggc ggcaaacaag atcgatgcgg
     3241 aggtcacgag gactggtgct tcgaatcaga caaacggtga acgccccgcc tttccggtgt
     3301 ctccgcaatg acattcacca accaccttta cgagccacac tgggtgtcat ctgtcatgaa
     3361 gcgctttgcg cgcgagccgc tgatccattt cctgcttctc ggcgccatcc tgtttggcgc
     3421 atacgcctat gtggagcgga aacaaggagg tgccgagcag tcaaacgaga ttaagctgac
     3481 aatcggcgat atttcgcagc tggttctggt gttccggtcg caatggcggc gcgatccaac
     3541 accgcaggag ttgcgacagc ttgttgaaga caaagtgcgc gaggaggtcc tttatcgcga
     3601 agcgctggcg ctgggactcg accgtgacga cacaatcgtt aagcgccgca tggcgcagaa
     3661 gatgcaattt ctcgccgagg acgtcgccgc cgcacgcgag cctacagtcg aggaactcgg
     3721 gccgtggtac gaggcgaacc acgaccagtt cgcgtttccg ccccgcgtaa gtttccgcca
     3781 tctctacttc tcagccgatc gccggggggc aagcgcgcgg gacgatgcag cgaccgcact
     3841 gacgaagctg gccgggcagc ccgagaactc gaacttcgcg gcgaccctcg ccgatgcatc
     3901 catgttccag gagtactacc gcgaccgcac accggagttt ttaggcaaag agttcggtcc
     3961 aaatttcgcg ctcaccgtcg tgaagttgcc gccggggtca tggcaaggac ctgtcgagtc
     4021 cgggtttggt tggcatctcg tcttcgtcga caagctgatt cccggccgcg tgcccccgtt
     4081 cgaagaggtt g
//
LOCUS       DATMGX010001050         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_185567, whole genome shotgun sequence.
ACCESSION   DATMGX010001050 DATMGX010000000
VERSION     DATMGX010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185567"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            562..1872
                     /gene="ltrA"
                     /locus_tag="VNR65_17270"
     CDS             562..1872
                     /gene="ltrA"
                     /locus_tag="VNR65_17270"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HWJ20470.1"
                     /translation="MSLETPEKIRSLQRKLYVKAKAEPDYRFYLLYDKVYREDILEHA
                     YKLAKANRGAPGVDGVTFAQIEEAGVKEWLSGLRQDLCEKRYKPQPVRRVMIPKPGGG
                     ERPLGIPTIRDRVVQTAVKIVIEPVLEADLSPYTFGYRPKKSALDAIKIVQGMLNQGY
                     TDVVDADLSKYFDTIPHSELLKSIAIRISDRNILRLIKMWLKAPVEERDENGGTRMSG
                     GKAAKMGTPQGGVISPLLANRYINRFLKYWAKTGQPRRLRARIVSYADDFVILSRGKA
                     AEALDWTRNAITRLGLTLNEAKTRLKDARRERFEFLGYSFGPHRHWRDGKPYLGASPS
                     PKSVARLKQNVRDHLQVSHCRPWPELRDGLNAILRGWDGYFCYGTRWKAHRAIDAHIV
                     QNVRGFLVRRHKVPTRGTRRFSREAIFEELGVFSLAANKEPCCR"
BASE COUNT          451 a          555 c          674 g          356 t
ORIGIN      
        1 ggaccagaaa taaatcgggc gcgccgaaat taaccatcag tttgttaagt gcgccacgag
       61 ggcgcggatg accagtggag gaaggttcca cccgaagagt agttggttag ctccggtagc
      121 tgacgggcgg ctttgccggg cgaccggctg ggccgaagcc ccgtgacaaa ggccgcgaag
      181 tgccggaggc acgccttcgg catgacggcg gtcgagcgac ctgacgggcc gtaatttgag
      241 tgaactctga gcaggcctcg aaagagttaa tgcggatgct gaccctcctt aatacagggg
      301 aaagcttgca ccggcgggga agcaaccaac aagcgcacct gccgggtccg ccggggtagt
      361 ggagacagca cgtcaggaag gtcatccggg taacgtggga gacccgtgcg gcagaggttc
      421 gcaacctgaa cgccaggaga tggcgggcca tgcgggagtc ggacaggcgc atagtaccgt
      481 cgaagccggg taactccggt ggagggaagg cgcctgactt ctggcacgct tttgaagaag
      541 gcaaggatca ggggattggc gatgagcctt gaaacgccgg aaaagatcag gagccttcag
      601 agaaagcttt acgtcaaagc gaaggcggag ccggactacc gcttctatct tctgtacgac
      661 aaggtctatc gcgaagacat cctggaacat gcctataagc tcgccaaggc caaccgaggc
      721 gcgcccggcg tggacggggt cacattcgcg cagatcgagg aggcaggggt caaagagtgg
      781 ctatccggct tacggcagga cctgtgcgag aaacggtaca aaccacagcc ggtacggagg
      841 gtgatgatcc cgaagcccgg cggcggcgaa cggcctctcg gtatcccaac catacgggac
      901 cgggtggttc agacagccgt caagattgtg attgaaccgg ttctggaggc ggacctgtct
      961 ccttatacgt tcggctatcg cccaaagaag agtgcgctcg acgcgatcaa aatcgtgcag
     1021 ggcatgctca accaagggta caccgatgtg gtggacgctg atttgtccaa gtatttcgat
     1081 accattcccc attccgaact tctcaagtcg atagcgatac gcatctcgga ccggaacatc
     1141 ctccggctca taaagatgtg gctaaaggct ccagtcgagg agcgggatga gaatggagga
     1201 acgcggatga gtggcggcaa ggctgcgaag atgggaacgc cgcaaggtgg cgttatctct
     1261 ccgcttctag ccaaccgtta cataaaccgc ttcctgaaat actgggcgaa gacgggacaa
     1321 ccgcgccgct tgcgcgcgcg gattgtctcg tatgccgatg acttcgtcat tttaagtcgc
     1381 gggaaagctg ccgaggcgct ggactggaca cgcaacgcga tcacccggct tgggctgacc
     1441 ttgaacgagg ccaagaccag gctcaaggac gcgcggcggg aacggttcga gttccttggc
     1501 tacagcttcg gtccgcaccg gcattggcgg gatggcaaac cgtatctggg ggcgtcgccg
     1561 tcaccgaaga gtgtggcccg gctcaagcaa aacgttcgcg atcatctaca ggtgtctcat
     1621 tgccggcctt ggccggaact ccgggacggg ttgaacgcga tcctgcgggg ctgggacggc
     1681 tacttctgct acggaacccg ctggaaagca caccgggcga tcgatgccca cattgtgcaa
     1741 aatgtgcggg gttttcttgt cagacggcac aaagtgccca cgcgaggcac tcgtcgcttc
     1801 agccgtgaag cgattttcga ggaactgggc gtcttcagcc tggccgcgaa taaggagccg
     1861 tgctgccgat agcgttgccg tgaagccagt cggaaagccg gatgctggaa atcggcacgt
     1921 ccggtttgac gagcggggag gggaaacggg gcgttgccgc atggcccaag ctaccgcgcc
     1981 tctcctcgac tctaccagat gccgcagggg ccgttcggcg gagcgcccgt tgtcgt
//
LOCUS       DATMGX010001051         4201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_212853, whole genome shotgun sequence.
ACCESSION   DATMGX010001051 DATMGX010000000
VERSION     DATMGX010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212853"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VNR65_17275"
     CDS             <1..1027
                     /locus_tag="VNR65_17275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWJ20471.1"
                     /translation="VETHRPTLISVVLPAYNECEGLPIVLSSIFGALAKTEFAHEIIV
                     VDDGSTDETRQIMLGLCSSNPNLHYVRLSRNFGKEAALSAGLKMASGDAIILMDADGQ
                     HPPTLIPAFLERWREGYEVVAGVQSARPESWLKRRFKGMYYHLLEAGSSVVIPPDAGD
                     FRLLDRKVVDAINALPERNRLMKGLYAWVGFRTALLPFQAQDRVAGQTKFTWMQLFRL
                     AFTGLTSFTVIPLRLVSVAGVVISCSSLLYGFYLLFEHYVLGDHLPGWATLSVGMMFS
                     SGVQLLALGIIAEYLGRTFEEVKQRPIFIIAEDSRQTAGTHAASPVYDATQLVTDAHL
                     PKTPAIP"
     gene            1029..1412
                     /locus_tag="VNR65_17280"
     CDS             1029..1412
                     /locus_tag="VNR65_17280"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWJ20472.1"
                     /translation="MRQIAIFIAVGSAAALTHLTVVALVVELLHLKPLAANVIGFCVA
                     FLVSFVGHARWTFPLSPERYTAARTRFFAVASTGFVLNQAAYAEVLYLFGPRYYLPAL
                     AAVLVGVAVATFLLSKLWAFAQPQG"
     gene            complement(2099..>4201)
                     /locus_tag="VNR65_17285"
     CDS             complement(2099..>4201)
                     /locus_tag="VNR65_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20473.1"
                     /translation="VNSPAGIKAIQYETRVAESPTLTILFNHPVSFNVRGGIDFQSLI
                     VSVSNNGKACKPEFPGRATDGWNAIPEGETVVARRQLPRETVVPKRAPNAIPIPDVAP
                     TEVPGSAQSSTENPQDGATGKLIADARAAMRQNNNQQAIALLRKAVAGPEDARSQEAR
                     ELLGVAYQKDKQIAAAKSVYEDYVRRYPNGEGTDGVRQRLMALETAQAQPTAALRAPV
                     SGPVEIGGGPVEGNRNGQAQPNTSYVTMSGSVSQFYIKDDSFRVVRDPTQALNLNATR
                     DDLATHQNTLMSNVDASATWGDGNVKSKFRFSGTEEHLFDRGDTDLWGISALYLDTSI
                     KDWGTTFRIGRQIQNSNGILGRFDGAVITYQWNPWFGVSAVGGSPVESRFDLPFKDEI
                     YFYGASANFTHLFGDLDASIYGIEQRARDYIDRQAVGTEWRYADQTKSAFLTVDYDTY
                     FNKLDAAIFTGSWTLPDKSSVRLTADYRKAPYLTAWNALQGQGYVSLYDLLKANTQAD
                     VRQMAIDRTATYESANVGYTRQLTDKLQINLDFTAAHIDGTIASYGVFATPDMGTEFY
                     SSAQLVGTSLVKDGDLYTAAFRYSDLKESDNYAIDLSTRYPISEEWNVAPRLTAGYRT
                     GKVASYDEYMVLPSLLVDYFWRKDLNLELEVGERWTWHNQGTAKTTENELFVTAGFRY
                     DFYADSREKCATWSMLCR"
BASE COUNT          795 a         1250 c         1194 g          962 t
ORIGIN      
        1 cgtggaaaca catcgcccaa cgctgatatc ggtcgtcttg cccgcttata acgagtgcga
       61 gggcctgccc attgtgctgt cttcgatctt cggcgccctc gccaagacgg agttcgccca
      121 tgagatcatc gttgtcgacg atggaagcac cgatgaaacg cgccaaatca tgctgggcct
      181 atgctcgtcc aaccccaacc tgcactatgt ccggctctcg cgaaatttcg gcaaggaagc
      241 ggctctctcc gcaggtctta aaatggcttc gggtgatgcc atcatcctga tggatgccga
      301 cggacagcat cctccgacac tcatcccggc ctttctggaa cgctggcgcg agggctacga
      361 ggttgtcgca ggcgtgcaaa gtgcacgtcc cgaaagttgg ctgaagcgcc gtttcaaggg
      421 catgtactat catctcttgg aagcggggag ctccgtggtc atcccgcccg acgccggcga
      481 cttccgcctc cttgaccgga aagtggttga cgcgatcaat gcgctcccag agcgaaaccg
      541 cctcatgaaa gggctttatg cctgggtcgg gtttcgcaca gcgcttttgc cctttcaagc
      601 gcaagaccgc gtggcgggtc aaacaaagtt cacgtggatg cagttgttca ggcttgcctt
      661 caccggcctg acctctttca ccgtcatacc tctgcgcctc gtttcggtcg cgggagtagt
      721 catttcctgt tcgtcgctcc tctacggatt ttaccttctt ttcgagcact acgtgcttgg
      781 cgaccatttg ccgggctggg ccacgttgtc ggtcggcatg atgttctctt cgggcgttca
      841 gcttctggcg ttgggcatca tcgccgagta tctcggccgc accttcgagg aggtgaagca
      901 gcgccccatc ttcatcattg ccgaggacag ccggcaaacg gctggaaccc atgcggcctc
      961 gcccgtctac gacgcgacgc aattggtgac ggacgcgcac ctgccaaaga cgccggcgat
     1021 cccctaagat gagacaaatc gcgatcttca ttgcggttgg ctccgcggcc gccctcaccc
     1081 atctcacggt agtggccctt gtggtggaac tcctccacct gaagccgctt gccgccaatg
     1141 tgatcggctt ctgcgtggcc tttctcgtga gcttcgtcgg ccacgcccgc tggacgtttc
     1201 ccctttcgcc ggagcgttac accgcagcgc ggacgcgctt tttcgccgtg gcaagcaccg
     1261 gcttcgtcct caaccaggcc gcttatgcgg aagtcttgta cctcttcggg ccccgctatt
     1321 atctccctgc cctcgccgcc gtccttgtcg gagtcgctgt cgcgacattc ttgctatcga
     1381 aactatgggc tttcgcgcag ccgcaaggtt agcgcggcgt gcgcactcgg aacgggttga
     1441 acgcaggatg ctttcaggca tgcttcgcct gggccatcac cacgtcggat agcgctcccc
     1501 gaacgcctcc tggtcatccg cgccttcgtg gcgcaagtaa acggttcgtt gccgggccgg
     1561 tcgttaattc gatattggtt aacggaagcg cgccggattt atttccgctc accattgtgc
     1621 ttaacaagca ttttattcct gcctcaatta cttcccgccg aggcttcgtc ccggtcggaa
     1681 gtaattctgc tatgtcgttg caagcaaaga gctttcactc gctcatggcc tcccattaaa
     1741 tagaattaaa tgcaggaatt ttttcattta ctgggacgag caaaagtccg ttaaatatgt
     1801 ttcggctttt gcgaattaac gctggcgcgg agtcggaaca gctgcggcca aggcagcggc
     1861 gatggcgtcc cgttcggtat ttttcccgcg attatctcgc tgttatcaag ggttggagcg
     1921 ggctcacgtt ctcgttaacc caacggcaaa gcgttttcgc cgatgatctt tcagaagaag
     1981 gaatgcggac gcaagtggcg gggagaaatt gacaaacaga tgccgacatt cgccagatgc
     2041 atacaaaaaa caccggacgc gcgcgtccga tcaagcgcac gatgctttaa agctgtaatc
     2101 aacggcacag catggaccac gtggcacact tctcacgcga gtccgcatag aagtcgtagc
     2161 gaaagccggc ggtcacgaat agctcgtttt cggtggtctt cgcggttccc tggttgtgcc
     2221 aggtccagcg ttctccgact tccaactcca gattgagatc cttccgccag aagtaatcaa
     2281 cgagcaagga cggcagcacc atgtactcat cataggaggc taccttcccg gtccgatagc
     2341 cagcggtaag ccgtggcgct acgttccact cttcgctgat cgggtaacgc gtgctgaggt
     2401 caatcgcgta attgtcggat tccttcaaat cggaatagcg gaaggccgcc gtgtacagat
     2461 ccccatcctt gacgagagag gtgcccacga gttgggcgga ggaatagaac tcggtgccca
     2521 tatcgggcgt tgcgaaaacg ccgtaggaag cgatcgtccc gtcgatgtgg gcagccgtga
     2581 agtcgagatt gatctgcagc ttgtccgtaa gctgtcgcgt gtacccgacg tttgcggatt
     2641 cgtaagtggc agtcctgtcg atcgccatct gcctcacgtc cgcctgcgtg tttgccttca
     2701 agagatcgta aagcgacacg tagccttggc cttgtaacgc attccatgcc gtcaagtacg
     2761 gggccttgcg gtagtcggca gtcagtcgaa ccgaggattt atcgggaagc gtccatgatc
     2821 ccgtaaagat ggcggcgtcg agcttgttga aataggtgtc gtagtcgaca gtcaggaacg
     2881 ccgatttagt ctggtccgca tagcgccact ccgtgcccac agcctggcgg tcgatatagt
     2941 cgcgcgcacg ctgctcaatt ccgtaaatgc tggcatccag gtcgccaaaa aggtgcgtga
     3001 aatttgcgct ggcgccgtag aagtagattt cgtctttgaa gggcagatcg aaccggcttt
     3061 ccacgggtga gccacccacc gcggacacgc cgaaccacgg attccactga taggtgataa
     3121 ccgccccgtc gaaccggccg aggataccat tcgagttctg gatctggcgg cctatccgga
     3181 aggtcgtacc ccaatccttg atggacgtat cgagatacag cgcgctgatg ccccacaggt
     3241 cggtgtcgcc acgatcgaag agatgctctt ccgttccgga gaaacggaat ttcgatttga
     3301 cgttcccatc accccaagtg gccgacgcat cgacgttcga catcagcgtg ttctgatgcg
     3361 tggccagatc gtctctggtg gcattcaagt tcagcgcctg tgtcggatcg cgaacgactc
     3421 ggaagctgtc gtctttgatg tagaattgcg agacgcttcc cgacatcgta acgtaggagg
     3481 tgttaggctg ggcctgtcca ttcctgttac cctcgaccgg accgcctcca atttcgacag
     3541 gtcctgatac cggtgcccgg agtgctgctg tcggctgggc ctgcgccgtt tcaagcgcca
     3601 tcaaacgctg gcgcacgcca tctgtgcctt ccccattcgg gtagcgccgg acgtaatcct
     3661 cgtagaccga ttttgcagcc gcgatctgct tatccttctg gtaggccacg ccgagcagtt
     3721 cccgggcctc ctgcgaacga gcgtcttccg ggccggcaac cgccttgcgc aacagggcga
     3781 tggcttgctg attattgttt tgcctcattg ctgcgcgcgc gtccgcgatc agcttgccgg
     3841 ttgctccgtc ttgcgggttc tctgtcgaag actgcgcgct gccgggaact tctgtcggag
     3901 caacatcggg gattgggatc gcgttcggcg cccgctttgg caccaccgtc tcgcgaggaa
     3961 gctggcggcg ggcgaccaca gtctcgccct caggaatcgc gttccagccg tctgtggcgc
     4021 ggcctgggaa ttcgggcttg caagccttgc cgttattgct caccgaaacg atcaggcttt
     4081 gaaaatcaat acctccgcga acgttgaaac tcacggggtg attgaacagg atcgtgagcg
     4141 tcggactttc ggcgacgcga gtctcgtatt ggatcgcctt gatcccggca gggctgttca
     4201 c
//
LOCUS       DATMGX010001052         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_122447, whole genome shotgun sequence.
ACCESSION   DATMGX010001052 DATMGX010000000
VERSION     DATMGX010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122447"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..747
                     /locus_tag="VNR65_17290"
     CDS             466..747
                     /locus_tag="VNR65_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20474.1"
                     /translation="MIKFCIAAMGALTMMSAPAFADYYIVHGPDRHCRVVERVPEGGD
                     FVRVGPLHFGTQEEAEGQVRVVCHDNGYYREEARRGQMREREDYREERR"
     gene            887..1540
                     /locus_tag="VNR65_17295"
     CDS             887..1540
                     /locus_tag="VNR65_17295"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWJ20475.1"
                     /translation="MSWKHCGPGRRGYHHGNLRETLIQSALSLIGEKGPAGFTIAEAA
                     RSAGVSPAAPYRHFRDREELMAEVARLGFERFANDLEKAWHGGNPSPLEALQNVGRAY
                     LAFARNEPAYYAAMFETGLPKAEALGLQESSNRAFDALSTATKKLIEKLPEDTRPPAQ
                     MVALHIWAMSHGIASLFGRGNANAGSAIAPEDLLEAGVLLYLRGLGLAGGAAPAATL"
     gene            complement(2019..>2565)
                     /locus_tag="VNR65_17300"
     CDS             complement(2019..>2565)
                     /locus_tag="VNR65_17300"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ20476.1"
                     /translation="QVQEMLFAETNRLERARHASVSRGLKAHIASLRKALAALEAEIA
                     AHVEASETLARKARLMRTLKGVGPVTVSAVLAYLPELGALTRSEAACLVGLAPINNDS
                     GKFNGPRHIEAGRAAVRKTLYMTALVAIRRNPVMRLFADKLRRNGKPSKVVIAAVMRK
                     LIVTLNAIVRSGQPWSHAQTA"
BASE COUNT          503 a          724 c          795 g          543 t
ORIGIN      
        1 atgccttcga cagcttcgat ggccgcgcgg aaccgctgct cgctttcgcg gaggcgctcc
       61 ttgtccgtga ttgcgatcgc agctccggaa tctgccgaaa gcgcctccgg ctccctctcg
      121 cggtcaggcc gttgagtcag ggctatgtcg ctcgttccca acttcaaatc ctttctaaga
      181 ggggctcggc agagagacgt tgcaagagcg aaatggcgat ttagagattt aacctaaact
      241 cggctttcag gttcccgacc atcccgaagt tgttggccca ggagattcaa cttatacgag
      301 aacagaatgt gaacatgtca acgcgagttg acaatagcgg aacgttttag gaacattctc
      361 ggaatgcggg ccggatcacg gcagaggatg cgtttcaaaa ggcatcaaag aaactttctt
      421 gaagaggact tgttgaaaaa agcagtcatc tcttggagaa gaacaatgat caaattctgc
      481 atcgcggcaa tgggtgccct cacaatgatg tccgcgcccg cattcgctga ttactacatc
      541 gtgcacggac ccgaccggca ttgccgcgtc gtcgagcgtg tcccggaggg cggcgacttc
      601 gtccgtgtcg gcccgcttca cttcgggacg caggaagagg ccgaaggtca ggtccgcgtt
      661 gtctgccacg acaatggcta ctatcgcgaa gaggcacgcc gcggccaaat gcgtgagcgc
      721 gaagactata gggaagagcg ccgctgacgt cgggggggtc gatctggttt gcacaacaag
      781 gaggcccgcg gttctgcggg cctttttttc gttgcagagt ccgtttctcg ccgcgcccgc
      841 aaataaatgc tgctcaaagg agtgccgcca tgtaaatctt cctcacatga gctggaaaca
      901 ttgcggccct gggcggcgcg gatatcatca tggcaatctg cgggagacgc tcattcagag
      961 cgcgctgagc cttatcggtg agaaagggcc ggccggtttc acgattgccg aagccgcgcg
     1021 ctccgcaggc gtcagcccgg ctgcgccgta tcgtcatttc cgcgaccgag aggaattgat
     1081 ggccgaggtg gcgcgcctgg gcttcgagcg ttttgcgaat gacctcgaga aagcgtggca
     1141 tggaggcaat ccatctccgc tcgaggcgct gcaaaatgtc gggcgcgcct atctcgcctt
     1201 cgcccgcaac gagcccgcct attacgcggc catgttcgaa acgggcttac cgaaagcgga
     1261 ggctcttggc cttcaggaat cgtccaaccg ggccttcgac gcgctgtcta cggcgacaaa
     1321 aaagctcata gaaaaattgc cggaagacac acgaccgccc gcccagatgg ttgcgctgca
     1381 catctgggcg atgtcgcacg gtatcgcttc gcttttcgga cggggaaatg ccaatgccgg
     1441 atcggcgatc gcgccggaag acctgctgga ggccggtgtc cttctttatc ttcgggggtt
     1501 ggggttggcc ggtggggccg cgcccgctgc tacactttga catatgcggc tgtgaacatg
     1561 ccgcagcttg aatatgcgtt gtttacaggc agttggttcg gctataggct ctatcatcaa
     1621 gcttagattg gctcgcctga agaggtgcgt cacgaggtgt tgcttcgcgc tcttatgatg
     1681 ccgatcaccg acttgaaact gcggttcaaa cacgggagaa aagcatgagt cctgcactga
     1741 tttgggttaa cataatcgtt ttttcggcgg tctacgcggc ttaccttctc agtttgccgg
     1801 ggactcctgg ccttccgtcg tcttcctatt atctcaatgg caaagggccg aagccgtaag
     1861 aaagtccaag ccgctccggt aatttccgga gatgcggaag acgggcgcgg gagcaagact
     1921 tgctccttgc gctatttttt tgccttaggc aaacgccacc aatctggcca aggcatagtc
     1981 atcccggaag ggcgaagcgc gatctaccgt cttcggagtc aagcggtttg cgcatggctc
     2041 caaggctggc cagaacgcac gatggcgttc aaggtgacaa tgagctttcg catgacggcg
     2101 gcgatgacga ctttgctcgg cttgccgttg cgcctgagtt tatcggcgaa gagccgcatg
     2161 acgggattgc ggcggatggc gacgagagcc gtcatgtaca gcgtcttgcg gacggcggca
     2221 cgtccggcct cgatatggcg aggcccgttg aacttgccgc tgtcgttatt gatgggcgcg
     2281 aggcccacaa ggcaggcggc ttcgctcctg gtcagcgcgc cgagttccgg cagataggcc
     2341 agcacagccg agacggtcac ggggccgacg cccttgaggg tgcgcatcag gcgcgccttg
     2401 cgcgcgagcg tttcgctggc ttcgacatgc gcggcgatct ccgcctccag cgccgccagg
     2461 gctttgcgca agcttgcgat gtgcgccttc aggcctcggc tgacgctggc gtgacgggcg
     2521 cgctccagcc ggtttgtttc ggcgaaaagc atctcttgga cctgg
//
LOCUS       DATMGX010001053         2804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_58198, whole genome shotgun sequence.
ACCESSION   DATMGX010001053 DATMGX010000000
VERSION     DATMGX010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58198"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VNR65_17305"
     CDS             <1..1322
                     /locus_tag="VNR65_17305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ20477.1"
                     /translation="TLDIPTGTRVLLYQNLDAKWNPQSLLDLNGPAAGVDKIAPLVAR
                     VRDTLQESQGVIHWTSDSADAEVFHAIPLTGPVFSAPHQSTQSQSAANRGGANQQLMG
                     VLLVGSSRRPLIELQRRVISTAMMVGGAGIFVAVLASLWFAARVTRPVVSLAQAARRV
                     AAGDLGAKVEVESSDELGELAASFNRMTEDLVQQKDRTLQAERVAAWRELARRLAHEL
                     KNPLFPLQVTVENLLRAKQKSPEMFEEVFHEGTATLLAEINNLKTIIGRFSEFSKMPQ
                     PQRRPTQVNDLVHSVLRVFHAQLQEKNQIAVRPELADALPEISVDPDLLHRALQNLVL
                     NAIDAMPQGGELTIRTATLGDRFEISVSDTGSGLTQEECGRLFTPYYTTKQHGTGLGL
                     AIVQSVVSDHGGKISVASTKEKGTTFRIELPCEPQRSAGVLAGHDGG"
     gene            1407..2789
                     /locus_tag="VNR65_17310"
     CDS             1407..2789
                     /locus_tag="VNR65_17310"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWJ20478.1"
                     /translation="MAQKAHLLIVDDEANTLASLSRAFRLEGHEAAVCDNANKALELA
                     KSQSFDLILSDVVMPGKDGLTLLEELKAQGVPTPVVMMSGQAHIEMAVRATRLGALDF
                     LEKPISTEKLLLTVENALKLGRLERDNRDLERRLGKHQIVWKGETMQRVMAQVERVAA
                     RETRVCILGETGTGKELVARTLHERSSRASGPFVTLNCAAVPAELIESELFGHEKGSF
                     TGAAGRHLGKFEQARHGTIFLDEIGDMPLTMQAKLLRVLEEGEVERIGGERAITVDVR
                     VVVATHRDLEAQVRDGKFRQDLFHRVFVFPLRLPPLRERREDIPALIEHFALQVSATN
                     GWKPMRFTPDAMAALQEHSWPGNVRELRNAVERLMLLATFDEVTSETVALALPTSMAT
                     PDSGLAGTGPLADRVRAFEKQTILAELKRTHYQISNAARALGLERSHLYKKAEQVGID
                     LKAVKNEADR"
BASE COUNT          613 a          839 c          835 g          517 t
ORIGIN      
        1 cgacgctcga tatccccacc gggacgcgcg tgctgcttta tcagaatctg gacgcaaaat
       61 ggaatccaca atcgctgctc gacttgaacg ggccggccgc gggtgttgac aagatcgctc
      121 cactagtcgc acgggttcgc gataccttgc aggaatctca gggcgtgatc cattggactt
      181 ccgattcggc cgacgccgag gtttttcacg ccatcccgct gaccggaccc gtcttcagcg
      241 cgccgcatca gagtacgcaa agtcagagtg cggcgaaccg gggcggcgcg aaccagcaac
      301 tgatgggcgt tctactcgtc ggcagttcgc gccgtccgct gatcgaattg cagcggcgag
      361 tcatttccac tgcaatgatg gtaggtggcg caggaatttt cgtggcggtg ctggccagcc
      421 tgtggttcgc agcccgcgtc acgcgtcccg tcgtatcgct tgcccaagct gcgcgccggg
      481 tcgccgcggg cgatctcggc gccaaggtcg aggttgaatc gagcgatgaa ctgggcgaac
      541 tggctgcgtc attcaaccgc atgaccgagg atctcgtgca gcaaaaagat cgcactcttc
      601 aggccgagcg cgtcgccgca tggcgcgaac tggcgcggcg actggcacac gaattgaaga
      661 atccactttt tcccttgcaa gtcacggttg agaatctgct gcgcgcaaaa caaaaatcgc
      721 ctgagatgtt cgaggaagtt tttcatgagg ggacggcaac cctgctggcg gaaatcaaca
      781 atctgaaaac gatcataggc cgcttcagtg aattctcgaa gatgccgcag ccacagcgcc
      841 ggccgacgca ggtaaacgac ttggtgcact cggtgctgcg cgtcttccat gcgcaactgc
      901 aggagaagaa tcagatcgcg gtgcgccccg aattggccga cgcgttgccc gaaatttcag
      961 tcgatcccga tctgttgcat cgtgcgttgc aaaacctggt gctcaacgcg attgacgcga
     1021 tgccgcaagg tggagagctg acgattcgca ccgcaactct cggcgatcgg tttgaaatct
     1081 cggtttccga taccggctcc ggtctgaccc aggaagaatg cgggcgcctg ttcactcctt
     1141 attacacgac caagcagcat ggcacggggc taggcctcgc gattgtgcaa tcggtggtaa
     1201 gcgatcacgg cggcaaaatt tccgtcgcga gcacgaaaga aaaaggaacc acgtttcgca
     1261 tcgaattacc gtgtgaacca cagcgtagcg ccggcgtcct cgccggccac gatggtggat
     1321 gaatagcagg aaagatcgag gacgctcgta ccttcatcgt tacacgtgaa cgaactttat
     1381 cggagttttc ttaacaggag aaaaccgtgg cgcaaaaagc gcacttactg atcgtcgatg
     1441 acgaagccaa cacgctggcc tcgctatcgc gggcgtttcg cctggaaggg catgaagccg
     1501 ccgtttgcga caacgcaaac aaggctctgg aactcgccaa atcgcaatct ttcgacttga
     1561 tactctccga cgtggtgatg cccggcaaag acgggctgac gcttctcgaa gaactcaaag
     1621 cccaaggcgt gccgactccc gtcgtaatga tgtcgggcca ggcacacatc gagatggccg
     1681 tacgtgccac gcgactgggt gcgctcgact tcctcgaaaa gcctatctcg accgaaaaac
     1741 tgctgctcac tgtggaaaac gcactcaagc tcggccgcct cgagcgcgac aatcgcgacc
     1801 tggaacgccg cctgggcaaa caccagatcg tctggaaagg cgagaccatg cagcgcgtga
     1861 tggcgcaagt ggaacgcgtg gctgcccgcg aaacccgcgt ctgcattctc ggcgagaccg
     1921 gcacgggaaa agaactggtt gcacgcacgc tgcatgagcg cagttcgcga gccagcggtc
     1981 cgttcgtcac gctgaactgc gccgcggttc cggcggaact gattgaatcc gaattattcg
     2041 gccacgaaaa aggatctttc accggcgccg ccggccgcca cctcggcaag ttcgagcagg
     2101 cgcggcacgg tacaattttt ctcgatgaaa tcggcgacat gccgctcacc atgcaagcca
     2161 aactgctgcg cgtgctggag gaaggcgaag tggagcgcat cggcggcgag cgcgccatca
     2221 cggtggatgt gcgagtcgtc gtcgccaccc accgcgacct ggaggcgcaa gtgcgcgacg
     2281 gcaaattccg gcaggattta tttcaccgcg tgttcgtctt ccccctgcgc ctgcccccgt
     2341 tgcgcgaacg ccgcgaagat atcccggcgc tgatcgagca ctttgccctg caggtgagtg
     2401 ccaccaacgg atggaagccg atgcgcttca ccccggacgc aatggccgcg ctgcaggagc
     2461 attcgtggcc gggcaatgtc cgcgaactaa gaaacgcagt cgagcggctg atgctgctgg
     2521 caactttcga tgaagtgact tccgaaaccg tcgcgctggc gctgcccact tccatggcca
     2581 cgcccgattc tggattggcc ggcaccggcc cgctagccga ccgcgtgcgc gccttcgaga
     2641 aacaaaccat tctcgcggag ttgaaacgca ctcactacca gatctcaaac gcggcgcgtg
     2701 cactgggact ggagcgatcg catctttata aaaaggcaga gcaggtaggg attgatttga
     2761 aagcggtcaa gaacgaggca gaccgctgac acctcatatt ggtg
//
LOCUS       DATMGX010001054         3982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_241954, whole genome shotgun sequence.
ACCESSION   DATMGX010001054 DATMGX010000000
VERSION     DATMGX010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241954"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VNR65_17315"
     CDS             <1..1052
                     /locus_tag="VNR65_17315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ20479.1"
                     /translation="EIGIKPDSKQMRQGYMDGPDGQILRVLERDIDFGQENESRVFSI
                     SVAFDAVEVDNQARHLTNTIVLTLALLGVGLLLATFFQVRFGLSPLKDIGKQIAAIRS
                     GEETKLEGTPPDEILPLQEELNALIEANKSVVERARTHVGNLAHALKTPLSVIINEAA
                     SQNTDFANKVAQQATLMKQQISHHLDRARIAAQIRPASGVTDVGVQLSALSRALQKIY
                     EARALHFQVECPGGVRFSGEKQDFEEMAGNLMDNACKWARSSVQVKVQAPDGSLPQGD
                     RTFELIIEDDGPGLPEDFRRSAVKRGRRLDESVPGSGLGLSIVADLSHLYRGSLRLEE
                     ATTGGLRACLTLPAA"
     gene            1133..1579
                     /locus_tag="VNR65_17320"
     CDS             1133..1579
                     /locus_tag="VNR65_17320"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HWJ20480.1"
                     /translation="MKKLGLIFISLGFAVLSAGCATKEDTGTLVGAGTGALIGNQFGR
                     GGGRVAATVAGAVVGGFVGNRIGASMDEEDRRRASEAQYAAFENGNRAEWRSPNGHYG
                     YIEPRPVYYYNDMTCREFTQTAYVDGKPATMTGRACRGSDGRWHEV"
     gene            complement(1623..2270)
                     /locus_tag="VNR65_17325"
     CDS             complement(1623..2270)
                     /locus_tag="VNR65_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20481.1"
                     /translation="MKALHHHPHGETLMSHAAGTLDPALAVVLSCHLQFCARCRAHLR
                     IMDDVGGVLLEQLKAPEDEDFFKRTMHRFGGEVAQDVGSVLPTKALDPGTEVLMPAPL
                     ARATGLRRETIPWRQMPHGLNQYDLPKFAGAAASSHIVHIEPDAILHSERHGGQLILV
                     LWGAYDYDGHRFERGDLHDISESGFKTFKGASAEGVTFFTSISPVPQLTIFRTGH"
     gene            complement(2267..2800)
                     /locus_tag="VNR65_17330"
     CDS             complement(2267..2800)
                     /locus_tag="VNR65_17330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWJ20482.1"
                     /translation="MDDLLQRIALHRDPQAFQDLFRLFGPRIKAMMMRQGADAAAAED
                     IAQEALFAVWRKAHLFVAQKGGVATWIYTIARNLRIDRIRRQAPFQDLPEEMYEIASD
                     DDLPDEALNRNQQRERIRKVLETLPPEQYEVVHLSYIEGLSHSEIASALGAPIGTVKS
                     RMRLAYEKIRDGVEEEK"
     gene            3221..3958
                     /locus_tag="VNR65_17335"
     CDS             3221..3958
                     /locus_tag="VNR65_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ20483.1"
                     /translation="MNEILALAELRNLRRYAYCILGNRRLSDIAVETSLNGLVSDVIS
                     VSGRAISRLDLYRKVNEAAVATPGNVSAGAASGLYAQFQRLSREQRQIAALRAVVGFP
                     YCDIASIMDMPEKVVRRTYCEALLTLRDKPAAVLIIEDEVFIALDLQQIVRNLGFSVA
                     GIAKNRAEALRIAELTKPKLILADYRLRDDTGVEVVKAIREQMNANVIYVTSHPEEVR
                     DCTDDLVISKPFNPRALAMAVQTHLAA"
BASE COUNT          808 a         1157 c         1156 g          861 t
ORIGIN      
        1 gcgagatcgg catcaagccc gactccaagc agatgcgcca aggctacatg gacggcccgg
       61 atggacaaat tctgcgcgtg ctggagcggg atatcgattt cgggcaggaa aatgagagcc
      121 gggttttctc catatcggtt gccttcgacg cggtcgaggt cgataaccag gcacgccatc
      181 tgacgaatac gatcgtgctg accctcgccc ttctcggggt aggtcttttg ctggcgacgt
      241 tcttccaggt tcgtttcggg ctcagtccgc ttaaggatat cggcaaacag attgccgcga
      301 tccgatcggg tgaagaaaca aagctggaag gcacgccgcc tgacgaaatc ctgcccctgc
      361 aagaggagct gaacgcgctc atcgaagcca acaaaagcgt ggtggagcgc gcccggaccc
      421 atgtcggtaa tcttgcccac gctctgaaga ctcccttgag cgtcattatc aacgaagcgg
      481 catcgcagaa taccgacttc gcgaataagg ttgcacagca ggccacgctc atgaagcagc
      541 agatttccca tcacctcgac cgcgcacgta tcgctgcgca gatccggccg gcgagcgggg
      601 tcaccgacgt gggcgtccag ctcagcgcgc tttcccgtgc cttgcagaaa atctacgaag
      661 ccagggcttt gcattttcag gtcgaatgcc cgggcggggt gcgcttttcc ggggaaaagc
      721 aggacttcga ggagatggcg ggcaatctca tggacaacgc gtgcaaatgg gcgaggtcca
      781 gcgtccaggt gaaggttcag gccccggacg gctctctccc gcagggcgac cggacatttg
      841 agctgatcat cgaagatgac ggccccggct tgccggaaga tttccggcgt tccgctgtca
      901 aacgtggccg ccgtctcgac gaaagcgtgc ccggctccgg ccttggcctg tccatcgtcg
      961 ccgacctgtc gcacctctat cgcggatcgc tccgtcttga agaagcgaca accggaggtt
     1021 taagggcctg cctgacgctg cctgccgcct gatgacgtaa aatcgccaga aacagccgcc
     1081 taggctggca caaagtggct aggagcggcg ggcgattcgc ctcgatgggg acatgaaaaa
     1141 gctaggtctt atttttatct cgcttgggtt cgcggtgctt tcggctggat gcgccacgaa
     1201 agaagatacg ggcacgcttg tcggggcggg aaccggcgcg ttgatcggca atcagttcgg
     1261 caggggtggc ggacgcgttg cggcgacggt cgcaggcgca gtcgtcggcg gcttcgtcgg
     1321 taaccggatc ggcgcctcca tggacgagga ggaccgccgg cgcgcttccg aagcgcaata
     1381 cgcggcgttc gaaaatggca atcgcgcgga gtggcgcagc cccaacgggc attatggtta
     1441 tatcgagccg cgcccggtct attactacaa cgacatgacg tgccgggagt ttactcaaac
     1501 cgcttacgtc gacggcaagc ctgcaacaat gaccggaagg gcttgcagag gatccgatgg
     1561 ccgatggcat gaagtataat tttccgcccg ccagcttgcg gtgacaaata acgcagtggc
     1621 ccttaatgcc cggtccggaa gatcgtcagt tgcgggaccg ggctgatcga ggtgaagaag
     1681 gttacgcctt ccgcgcttgc ccctttgaag gttttgaagc cgctttcgct gatatcgtga
     1741 aggtcgccgc gctcgaaacg atgtccgtcg taatcgtaag ccccccatag aaccaggata
     1801 agctggccgc catgtcgttc cgagtgaaga atggcgtccg gctcgatatg gacgatgtgc
     1861 gaagacgccg ccgcgccggc gaatttcggc agatcgtact ggttcagccc gtgaggcatc
     1921 tgtctccacg gaattgtctc gcgccgcaga ccggtcgcgc gcgcaagtgg ggccggcatg
     1981 aggacctcag ttccgggatc aagtgctttg gtaggcagca cactgcccac gtcttgcgca
     2041 acctccccgc caaaacggtg catcgtccgt ttgaaaaaat cttcgtcttc cggcgctttg
     2101 agctgctcca gaagaacccc gcctacgtca tccatgatgc ggagatgcgc ccggcatctc
     2161 gcacaaaact gaagatgaca actcaaaacg accgccagcg cggggtctag cgtacccgcg
     2221 gcatggctca tcagcgtttc gccgtgggga tggtggtgga gggctttcat ttctcctcct
     2281 ccactccatc ccttatcttt tcataggcga gccgcatgcg tgatttcacc gttccaatag
     2341 gagcgccgag cgccgatgcg atctcgctgt gggagagccc ttcaatataa gagaggtgga
     2401 cgacttcata ttgctccggc ggcaaggtct caagaacctt ccggatacgt tcgcgttgct
     2461 ggtttcggtt caatgcctca tcgggcaaat cgtcatcact cgcaatctca tacatctctt
     2521 cgggcagatc ctgaaatggc gcctgccgtc gtatccggtc gatgcgcaga ttccttgcga
     2581 tggtatagat ccaggtcgcc actccccctt tttgcgcaac gaaaagatgc gctttccgcc
     2641 acaccgcgaa aagggcttcc tgcgcgatgt cttccgcggc ggcggcatcg gccccctgcc
     2701 tcatcatcat cgccttaatg cgcggtccga aaagccggaa caggtcctgg aaagcctgcg
     2761 gatcgcggtg gagcgcaatc ctttggagaa gatcatccat gccggattaa tgacaaaagc
     2821 gggttgccgt tacaaataac ttataagcga tggccaaggt ccctccgctt atcgcagaag
     2881 cgctgatgcc attggcatga gttttgctaa catttcctcc agcgatgcgc tgctacttgt
     2941 cagagagctg cgtgacgtga cccaagttgg aacgcgaact cctccgccgg cttgcttcag
     3001 ggtgtctccc ttgccctgat gcgccggcgt ttttttttag tcacgctctc gcttttttct
     3061 aaaacgtaaa cgaattagac attttcaggg aaaagtcatc gtctttcctc tttcccctct
     3121 cctttcttag ctgtatgttg accttagaag tgcgcggagt ggagtttctt cagctgctgc
     3181 tgaatttctg cgcaatggat ccttgagggt gccgcccata atgaacgaaa ttctcgcttt
     3241 ggctgagctg agaaacctac ggcgatatgc gtattgcatt cttggcaacc gacggttgag
     3301 tgacattgcc gtcgaaacct ccctcaatgg ccttgtttcc gatgtcattt ctgtcagtgg
     3361 ccgcgcgatt tcccgtctcg atctttacag aaaggtcaac gaggccgctg tcgccacgcc
     3421 cgggaatgta agtgcaggtg ccgcaagcgg gctctatgct cagttccagc gcctgtcgag
     3481 ggagcagcgc cagatcgcag cccttcgcgc tgttgtgggc tttccttact gcgacatcgc
     3541 ctccatcatg gatatgccag agaaagtcgt ccggcgtacc tactgcgaag cgctgttgac
     3601 tttgcgggat aagccggcgg ccgtccttat catcgaggac gaggtcttta tcgctctcga
     3661 ccttcagcag attgttcgga acctcggttt ctcggttgca ggcattgcca aaaaccgcgc
     3721 cgaggctttg cggattgcgg agcttacaaa gccgaagttg attctcgccg actacaggct
     3781 gagggacgac acaggggtcg aagtcgtcaa ggccattcgg gagcagatga acgccaacgt
     3841 catctatgtc acctcgcacc ccgaggaagt ccgtgattgc acggatgacc tcgtcatctc
     3901 gaagccgttc aatccacgcg ccctcgcgat ggccgtgcaa acgcatttgg cggcgtaaat
     3961 tcttcgccat cctgaggccg ct
//
LOCUS       DATMGX010001055         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_213208, whole genome shotgun sequence.
ACCESSION   DATMGX010001055 DATMGX010000000
VERSION     DATMGX010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213208"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1727)
                     /locus_tag="VNR65_17340"
     CDS             complement(117..1727)
                     /locus_tag="VNR65_17340"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HWJ20484.1"
                     /translation="MAGLTRSSHGSTVRGLTLSRGSKFFGGQFGRMFRALPPAEFGES
                     DTKSEENLLNLGKAMVADHDPPKDGPDAEESGIPAAYTYLGQFIDHDLTFDPASSLQQ
                     QDDPDGLVDFRTPRFDLDCVYGKGPDDAPYLYSSDNLFIQGRPLTGSASNPNARDLPR
                     SQPTDPSQPEDINNSRRAIIGDPRNDENVIVSQFQGLMHRFHNKLMLEDTGPAGSRFQ
                     RVQREVRFHYQWMLVNDFLPTIVWEDVLKDVIPHHFNDTNVRRDPPRITYFDLAHEPT
                     MPLEFSAAAYRFGHSMVRPGYRLSETVPPLAIFDPNPNASLTGFHEFRSNWAFDWGRF
                     MDLGPLDFGDENNPNDPGNSNRLQLAYRIDTSLVDPLGSLPNPPFAPPFSLSGGKLMS
                     LAQRNLMRGWRMRLPSGQAIARAMGIEPLKDADILIGKFTGDPTDIKGSVTSISAAFE
                     DNCPLWTYVLAETKEVTVKIKTNQGDKPIKTRRLGPVGGRIVAETFIGLMLSDSSSYF
                     NQNPIWAPSKANAQGRFGLRELIKTALS"
     gene            complement(2336..>2533)
                     /locus_tag="VNR65_17345"
     CDS             complement(2336..>2533)
                     /locus_tag="VNR65_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20485.1"
                     /translation="RGGGFRGAGLGIGLGLGLAGAYGAYGYPYGYGYDSYAYDDGGCY
                     LVRQRVLTPYGWRLRRVQVCN"
BASE COUNT          569 a          698 c          711 g          555 t
ORIGIN      
        1 actaacttgg ccttcctacc taaaccggga gctgcgctcc cgccggtcga acgccgccgc
       61 cgcaaacggc ccgttgtcga agcctgaggt ctggcgcaaa ctacggcaaa tttcattcag
      121 gacagcgccg tcttgatcag ctcgcgcaat ccaaaccgtc cctgagcatt ggctttgctc
      181 ggggcccaaa tggggttttg attgaaatac gagctgctgt ccgacagcat gaggccgata
      241 aaggtctcag ctacgatgcg gccaccaacc gggcccagcc ttcgtgtttt aattggctta
      301 tcgccctggt ttgttttgat cttcactgtc acttccttgg tctccgcaag aacgtaagtc
      361 catagcgggc aattgtcctc gaacgccgcg gagatcgacg tgacggatcc cttaatgtcg
      421 gttggatcgc cggtgaattt gccgatcaga atgtcggcgt ccttcaaagg ttcgatgccc
      481 atggcccgag cgatggcctg cccgctcggc agcctcattc tccaaccgcg catcaagttg
      541 cgctgtgcaa gagacatcaa cttgccacca ctaagagaaa aaggcggggc gaacggcgga
      601 tttgggaggc tgcccagcgg atcgaccaaa gaggtatcga tcctgtaagc caactgaagg
      661 cggttgctgt ttccagggtc gttcggatta ttttcgtcac cgaaatcgag aggcccaagg
      721 tccataaacc gaccccaatc gaacgcccaa ttgctgcgga attcatggaa tccggtcagc
      781 gaggcgttcg gattgggatc gaatatcgcc aatggtggaa cggtctcgct gagacgatag
      841 ccaggtcgca ccatggagtg gccgaagcgg taagcagctg ctgaaaattc cagcggcatg
      901 gtcggttcat gcgcaaggtc gaaataagta attcgcggcg ggtcacgcct tacgttcgta
      961 tcattaaagt gatgaggaat gacgtccttc agaacatctt cccagacgat ggtcggcaga
     1021 aagtcgttaa caagcatcca ttggtagtga aaccggacct cacgctgaac gcgctgaaat
     1081 cgcgatccgg cgggtccggt gtcttccaac atcaatttgt tatggaatcg gtgcatcagc
     1141 ccctgaaatt gcgaaacgat gacgttctcg tcgtttcgtg ggtccccgat gatggcacgc
     1201 cgagagttgt tgatgtcttc gggctgcgac gggtccgtcg gctgactgcg cggcaggtcg
     1261 cgcgcattcg gattgctggc cgaaccggtg agcggccgac cctgaatgaa tagattgtcg
     1321 ctgctgtaga ggtagggcgc gtcgtccgga cccttgccat agacgcagtc taaatcaaaa
     1381 cgcggcgtcc gaaagtccac taggccgtcg ggatcgtctt gctgctgcaa gctgcttgcc
     1441 ggatcgaacg ttagatcgtg gtcgataaac tggccgagat aggtataggc cgcaggaatg
     1501 ccactctctt cggcgtcagg tccatctttg ggtggatcgt ggtccgcgac catcgccttg
     1561 ccgagattta gtagattctc ctcggatttg gtatcacttt cgccaaactc ggcaggcgga
     1621 agcgcccgaa acatccggcc aaattggccg ccgaagaact tcgagccgcg cgacaaggta
     1681 agcccacgga cagtactgcc gtgcgaactg cgagtaagac cggccatcgg tttcctccaa
     1741 gtttacagaa gttgttgtat tcaattatgc tgctaaaagt tgatattttg gctagggaaa
     1801 atcttcaacg aacagatgcg aatcgaatcg ccagattttt cggcttcttt tcgaaggacg
     1861 cgggcatatg cggcattcga tgcggcaatc caatgtgtag cgcactcgtc aaaatagcga
     1921 tgtgaactac ttcacaactg caacggtgcg aggcgacaac ggcaggcggt gatgcgcctc
     1981 ataagtgacc ttcgcgagcg cggtcgctgc gaaagcaaag gctgatgatt ttcgttttcc
     2041 ccggaaaagc gcgcaacaag cgctccccga catcgatcta ttggtcgtct ggagtacgat
     2101 cggaggagtt gcggcaaaga aattagcttc gtcggtaccg gtcgtatttc tctctgtcgg
     2161 ctggggccgt cacctttccc agacaaaaaa gagaccgggc gaatgtggac gcccggtctc
     2221 ttgagttagg tagatatcca aacgccagaa gcttacttgt ggcggttgta gagttgaact
     2281 ccccgcttgg cgtgccacga tcgacttctc gcggttcgct taacttcaat cagcgtcagt
     2341 tacatacttg gacccggcgc aatcgccaac cgtatggcgt caatacccgc tgccgaacca
     2401 aatagcagcc gccgtcgtcg taagcgtagc tatcgtagcc atagccataa ggatagccgt
     2461 aagctccata agcccccgcc aagccgagac ctagaccgat gccaagtccc gctcctcgga
     2521 acccaccgcc gcg
//
LOCUS       DATMGX010001056         3288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_90599, whole genome shotgun sequence.
ACCESSION   DATMGX010001056 DATMGX010000000
VERSION     DATMGX010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90599"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VNR65_17350"
     CDS             <1..393
                     /locus_tag="VNR65_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWJ20486.1"
                     /translation="TVRLVSQEVNTSSRLGRVRIFLGDAADLRIGMFAKAMIDAGQRD
                     AVGVPSTAVLYQNDSATVLAVLDGKVRERAVKPGLLAGDSVEIKEGLREGEIVVMRAG
                     SLLRSGDIISPQFAEKQTAQRAGIGVSQ"
     gene            390..>3288
                     /locus_tag="VNR65_17355"
     CDS             390..>3288
                     /locus_tag="VNR65_17355"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWJ20487.1"
                     /translation="MNISAWSIRRPIPSLVLFLVLMTLGLLSFRNLAITRFPNIDIPI
                     VTVDITQSGAAPVELETQVTKRVEDSVAGVTNVKHIKSSITDGASMTTIEFQLGTNTD
                     RAVNDVKDAVSKVRQELPRTINEPIIQRLDIEGLPIVTYGASAPSMTLEELSWFIDDT
                     VARKLQGVRGVAQVSRVGGVKREIRVSLIPERLLALGITAGEVNQQLRATNTDLAGGR
                     GEVGGAEQSIRALASASTVERLASTSITIPGGRKVRLDQLGQVTDAAQEKRTFAKLDG
                     RDVVAFTVVRAKGESDLSVAKRVSDAIQKLAEANPRVTFEKIDSSVDFTIGNYNSAMD
                     TLLEGAILAVLVVLLFLRDLRATIIAAITLPLAIFPTFWAMQALGFSLNMVSLLAITL
                     VTGILVDDAIVEIENIVRHMRMGKSAYRAALEAADEIGLAVIAITFTIVAVFAPVSFM
                     GGIAGQYFRQFGLTVAAAVLVSLLVARLITPMIAAYFLRPHDKVDREGLVMRNYTRLI
                     GWTVRHYYATLVLGFVVFALSIGSVFLLPSGFLPAEDTGRSIFAIELPPGSQLGDTEV
                     IAEKAENIIRRRPEVRSIFVDGGRILGDTGGAQEVRKSTLTVHYAPREDRDLTQRQLE
                     QIISADLKEIPDIRTWVVKENGARAFTIEVTGTDNEKVIETAAQLTSEIRTVPYLANV
                     VNGAALDRPEIQVTPRPVIAAELGVSTESLSDVIRIATLGDIDANLAKFNAPDRQVPI
                     RVQLDEQARSSLQTLQILKVKTGRGDAVPLITVADVKMGKGPSSIARYDRLRRVAIGA
                     DLVGTDALGQALSAVYALPAAKNLPPGVVLRESGDVEVMTETFQGFGYAMGAGVMMVY
                     SVLVVLFGSFLQPITILLSLPLSIGGAIFALLLTDRPISLPVFIGFLMLMGIVTKNAI
                     MLVDFAIVELRSGVPRRMAIIEAGRKRARPIVMTTIAMIGGMFPSALALGDG"
BASE COUNT          603 a         1010 c         1020 g          655 t
ORIGIN      
        1 accgtgaggc tcgtctcaca ggaggtcaac acgtccagcc ggctgggccg cgtgcgcatt
       61 ttcctcggcg acgcagccga tttgcgcatc ggaatgtttg cgaaggccat gattgacgca
      121 gggcagcgcg acgcggtggg cgttccttca acggccgtcc tctaccagaa cgacagcgcg
      181 accgtactcg ctgtcttgga cggcaaagtg cgcgagcgcg ccgttaaacc cggtctcctc
      241 gccggcgata gcgtggagat caaggaaggg ctgagagaag gcgagattgt cgtgatgcgg
      301 gcgggctcgc ttctgcgcag cggcgatatc atctctcccc aatttgccga aaagcaaacc
      361 gcccaaagag ccggcattgg ggtgagtcaa tgaacatttc agcgtggtcg atccggcggc
      421 cgatcccatc gctcgtgctt ttcctggtgt tgatgacgct gggtcttctc agctttcgaa
      481 acctggccat cacgcgcttt cccaacatcg acatcccgat tgtcaccgtt gacatcacac
      541 agtctggcgc ggctccggta gagctcgaaa cgcaggtgac aaagcgcgta gaagactcgg
      601 tggcgggcgt cacgaacgtc aagcacatca agtcctcgat cacggacggc gcttcgatga
      661 cgacaatcga gttccagctc ggcaccaaca cggaccgagc cgtcaacgat gtgaaggatg
      721 cggtctcgaa agttcgccag gaacttccac ggacaatcaa cgagccaatt atccagcgcc
      781 tcgacatcga gggcttgccc attgtgacat atggcgcttc cgcgccatcg atgacactgg
      841 aggagctttc ctggttcatt gacgacaccg ttgcccgcaa gctccagggc gtgcgcggcg
      901 ttgcgcaggt ttcgagggtc ggcggcgtga aacgcgagat ccgcgtttct ctcattcctg
      961 agcgactcct tgcgctcggc attaccgcag gcgaagtcaa tcagcagctt cgcgccacga
     1021 ataccgacct tgcgggcggc cgcggcgaag tcggcggggc tgagcaatcg atccgcgcgc
     1081 tcgcaagcgc gagcaccgtc gagcgtctgg cctcgacttc gatcaccatt ccgggcggac
     1141 gcaaggtgcg cctcgatcaa ttggggcagg taacagacgc ggcgcaggaa aagcggacct
     1201 tcgctaagct cgatggccgg gacgttgtgg ccttcacggt ggtacgggcc aagggcgaga
     1261 gcgatctgag cgtggccaag cgcgtttcgg acgccatcca aaagctcgcc gaggcaaacc
     1321 cgcgcgtgac tttcgagaag atcgactcct cggtcgattt caccatcgga aattataact
     1381 cggcgatgga tacgctgctc gaaggagcga ttctggccgt tcttgtggtg ctgctctttc
     1441 tcagggattt gcgagccacg atcattgcgg ccattaccct tccgctagcc atcttcccca
     1501 ccttctgggc gatgcaggcg ctcggcttct cgctcaacat ggtttcgctg ctggcgatca
     1561 cgctcgtgac cggcattctc gtcgacgacg ccatcgtcga aatcgagaac attgtgcgcc
     1621 acatgcgcat ggggaaatcc gcttaccgcg cggcgcttga ggccgccgac gagatcggcc
     1681 tcgcggtgat cgccattacc ttcaccatcg tcgcggtgtt cgcgccggtg agcttcatgg
     1741 gcggcatcgc cgggcagtat ttccggcaat tcggcctgac cgtcgccgct gctgttctcg
     1801 tttcgctgct cgtcgcgcgg ctcatcacgc cgatgatcgc ggcctatttt ctgcgcccgc
     1861 acgacaaggt cgaccgcgag ggtcttgtca tgcgcaatta cacgcgcctg atcggttgga
     1921 ccgtccggca ctattacgcg acgctcgttt tgggcttcgt ggttttcgcc ctgtcaattg
     1981 gcagcgtgtt cctgctgccg tcaggcttct tgccggccga ggataccggg cgttcgattt
     2041 tcgccatcga actgccgccc ggctcgcagc ttggcgatac ggaggtgatc gccgaaaagg
     2101 ccgaaaacat catacggcgc aggcccgaag tgcgatccat attcgtcgac ggaggccgga
     2161 ttctgggcga caccggcggc gcgcaggagg ttcggaagtc gacgctgacg gttcattatg
     2221 cgccccgcga agaccgcgac ctgacccagc gtcagcttga gcaaatcatc agcgcggacc
     2281 tgaaggaaat tccggatatc cgaacctggg tcgtcaagga aaacggtgcg cgcgccttca
     2341 cgatcgaagt gaccggcacg gacaatgaga aggttatcga gaccgcggcg cagttgacga
     2401 gcgagatccg cacggtgcct tatctggcca acgtcgtgaa tggcgcggcc ctcgacaggc
     2461 ccgagatcca ggtcacgccg cgtcctgtca tcgcggccga gcttggcgtc tcgacggaaa
     2521 gcctgtcgga tgtgatccgc atcgcgacgc ttggcgatat cgatgccaac cttgccaagt
     2581 tcaatgcacc ggaccggcag gtgccgatcc gcgtccagct cgacgagcag gctcgctcca
     2641 gtctccaaac cttgcaaatt ctgaaagtga agacgggccg cggcgacgcc gtccctctta
     2701 ttacggttgc cgatgtcaaa atgggcaaag gcccctcatc catcgcgcgc tacgaccggc
     2761 ttcggcgcgt cgccatcggc gcggatctgg tcgggactga tgcgctcggc caggcgctga
     2821 gcgccgtgta cgcgcttccg gcggctaaga acctgcctcc cggcgttgtc cttcgcgaat
     2881 cgggtgacgt ggaggtgatg accgaaacct tccaggggtt cggatacgcg atgggcgcgg
     2941 gcgtgatgat ggtctatagc gtgctcgtgg tcctgttcgg gagcttcctg cagccgatca
     3001 cgatcctgct ttcgctgccg ctctcgatcg ggggcgcgat tttcgcgctt ctcctgacgg
     3061 acaggcccat cagccttccg gtcttcatcg gcttcctgat gctcatgggc atcgtgacca
     3121 agaacgccat catgctcgtg gattttgcga tcgttgagct gaggagcggt gttccccggc
     3181 ggatggcaat catcgaggca gggcgaaagc gggcacggcc catcgtcatg acgaccatcg
     3241 ccatgattgg cggcatgttt ccgagcgctc tcgcgctggg ggatggcg
//
LOCUS       DATMGX010001057         3524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_267704, whole genome shotgun sequence.
ACCESSION   DATMGX010001057 DATMGX010000000
VERSION     DATMGX010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267704"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VNR65_17360"
     CDS             complement(<1..693)
                     /locus_tag="VNR65_17360"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWJ20488.1"
                     /translation="MDWDKLRIFHAAAEAGSFTHAGETLSISQSAVSRHISSLERDLN
                     VPLFHRHARGLVLTEQGELLYRTAQEVFSKLATTQILLADSRGKPNGELKVTTTRALG
                     TGWLTPRLKEFMKLYPDVRLQLLLHDEILNLTMREADVAIWFGPPTQQDLVRRKLFTV
                     HYHVYASTDLVKRYGKPRSVEDLDRHSILTYGGILPSPFKEMNWLETMGRDSKGSREP
                     ALRINSLVALREA"
     gene            complement(718..1680)
                     /gene="trxB"
                     /locus_tag="VNR65_17365"
     CDS             complement(718..1680)
                     /gene="trxB"
                     /locus_tag="VNR65_17365"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWJ20489.1"
                     /translation="MAPRHDRLVIIGSGPAGYTAAIYAARAMFRPVLIEGIQPGGQLT
                     TTTDVENYPGFADVIQGPWLMEQMRTQALHVGAEILQDYIVSVDFSNSPLLLNGDSGT
                     VYHTDAVIISTGAQARWLGLPSEQHYRGYGVSACATCDGFFYRGKDVLLVGGGNTAVE
                     EALFLTKFARSVTVVHRRNSFRAEKILQNRLFANPKIKVIWNATLDEVVGTESPKSVT
                     AAKLRDILTGEITQIRVDGVFVAIGHEPATALFKDQVEMTPSGYIVTAAKSAATNVAG
                     VFAAGDVTDDTYRQAVTAAGMGCMAALDAERYLASLETVAQAAE"
     gene            1939..2406
                     /locus_tag="VNR65_17370"
     CDS             1939..2406
                     /locus_tag="VNR65_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWJ20490.1"
                     /translation="MPAKLDATDWRILAELQKDGRITNVELASRVGLSAPPCLRRVRE
                     LERSGVIAGYHAVFNLPRLGFHLTAFAFVRLESQAEGDLLAFEARARQWDIVREAYML
                     SGDTDFLLKCVAPDLVTFQNFIIRELTAAPNVSSVKTAFTIKTAKSEPGAAIE"
     gene            2708..3469
                     /locus_tag="VNR65_17375"
     CDS             2708..3469
                     /locus_tag="VNR65_17375"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWJ20491.1"
                     /translation="MHGTIENSHQVHREGEDLANALDSALGATDDLAARVRAIEVVIA
                     GRIIFSTSLGLEDQVLLHAIVAAKAEIESITLDTGRHFPETYDTLTRTERLLGTRIRV
                     VMPDAEDVEDLVARDGLFGFRYAVEKRKACCHARKVLPLRRALSSAAGWVTGVRREQS
                     ETRDATPFATFDASFNLIKVNPIADWTLDRVEAYVAEHRVPVNALHAQGFPSIGCQPC
                     TRAIGPGEPIRAGRWWWEDETRKECGLHNRPGFAK"
BASE COUNT          731 a         1031 c          985 g          777 t
ORIGIN      
        1 ggcctcgcgt agcgccacca gactattaat ccgaagcgca ggttcgcgcg agcctttgct
       61 atcgcgcccc atcgtttcca gccagttcat ttccttgaac gggctcggaa gtataccgcc
      121 ataggtcaga atgctgtggc gatcgaggtc ttcgacgctt ctgggcttgc cataccgctt
      181 cacaagatcc gtcgaagcgt acacgtggta gtgcacggtg aacagcttac gccggacgag
      241 atcctgctgg gttggaggtc cgaaccatat ggcaacatcc gcctcgcgca tcgtgaggtt
      301 caggatctca tcgtgcaaga gaagctgtaa ccggacatcc ggatagagct tcatgaactc
      361 cttgagccgc ggagtcagcc agccggtccc gagtgccctg gtcgtggtaa ctttgagttc
      421 cccattcggc ttcccgcgcg agtctgcgag caaaatttgc gtggttgcga gtttgctgaa
      481 aacttcttgc gcggtgcggt aaaggagttc tccctgctcg gtcagcacga gtccgcgcgc
      541 gtgccggtga aacagcggaa cgttgaggtc gcgttcaagt gagctgatgt ggcggctgac
      601 cgccgactgg ctgatgctca gcgtctcgcc agcatgcgtg aaacttcctg cttccgctgc
      661 agcgtggaaa atccgaagtt tgtcccaatc catcggcaga ttgccgcccc ctctgcccta
      721 ttccgctgcc tgcgccaccg tttccagact tgccaggtag cgctcggcat cgagcgcagc
      781 catgcatccc atgcccgccg ctgtgacagc ctgacggtat gtgtcatcag tgacatcgcc
      841 cgcagcgaag actccggcga cattggttgc cgccgacttt gctgccgtaa cgatataacc
      901 gctgggcgtc atttccacct gatctttgaa cagcgctgtc gccggctcat ggccaatggc
      961 gacaaatacg ccatccaccc ggatttgagt aatttctcca gtcaagatat cgcgaagctt
     1021 agccgctgtc accgacttgg ggctttccgt tcccacaacc tcgtccaaag tcgcattcca
     1081 gatcactttt atctttggat ttgcaaatag cctgttttgc aaaatctttt ccgcacggaa
     1141 cgaattgcgg cggtgcacaa cggtcaccga ccgggcaaac ttggtgagga agagcgcttc
     1201 ctccacggcc gtattgcccc cgccgacaag gaggacatct ttgcccctgt agaagaagcc
     1261 gtcgcaggtg gcgcaggctg acacgccata gccgcgatag tgctgctcgc tcggaaggcc
     1321 aagccagcgg gcctgagctc ccgtggagat aatgacggca tcggtgtggt agacggtccc
     1381 gctatcgccg ttgaggagga gcggcgagtt cgagaaatcg accgaaacga tatagtcctg
     1441 gaggatctcg gccccgacat gcagggcctg cgtgcgcatt tgctccataa gccacggccc
     1501 ctggatgacg tccgcaaagc ctggataatt ttccacatcc gtcgtggtgg tgagctggcc
     1561 gccgggctga attccctcga taagaaccgg ccgaaacatg gcccgtgctg cataaatcgc
     1621 agccgtgtag cctgccggac cggatccgat gatcacgagc ctgtcgtgac gtggggccat
     1681 ttgaatgtcc tcttcaatct tttcaataca ctcggcaaat ggtttcgctg atcatccctg
     1741 ccaagacgaa tatgtcaagt tgaggcttcg ggagcgtctt cagagggctc aggcttgagc
     1801 ttttcgccgc aggctgtgca ttcgggttct tttattgttg cattgcagca tatactgcac
     1861 caactatttc aacgaatggg attaaccgca caaaaggaaa tccccatggc gtctgtacga
     1921 acattggacg agtcgaccat gcccgcgaaa cttgacgcga cggactggcg aattcttgcc
     1981 gagctgcaaa aagacggacg catcacgaat gtcgagcttg cctcgcgggt cggtttgtca
     2041 gcgcctccgt gcctgcggcg tgtccgcgag ctagagcgtt ccggcgtcat agcgggctac
     2101 cacgccgttt tcaatcttcc ccgccttggc tttcatctca ccgccttcgc tttcgtcagg
     2161 cttgagagtc aggctgaggg ggatctcctc gcattcgaag cgcgcgcccg ccaatgggat
     2221 attgtgcggg aagcctatat gctttccggc gatacggact tcctcttgaa gtgcgtcgcg
     2281 cccgacctgg tgacattcca gaatttcatc atccgagaac tgactgcagc gccaaatgtg
     2341 agttccgtca agacagcctt caccatcaag acggcaaaaa gcgagccggg cgccgcgatc
     2401 gagtaaatga ctttcgtagg caacagccaa cagtaaaaag gtccacgcaa gcggagattt
     2461 ccgatacgga ttttctggct tggggacaag aaccgtaaat tcattcttat ttttatgccg
     2521 ctccgtcagc acggtttttt ccgtgtattt tgacgtgcat attgtgggga accggctacc
     2581 gcattccgag aactcaaggg atcacctttc tgtcccattc ggaacagcgg ttgtgccatg
     2641 cccataagac ggtgatctcg aaaagcgaac gaggctttct cgacccgtga cgaaagagga
     2701 aacgacaatg cacgggacaa tcgagaattc tcatcaggtt catcgcgaag gcgaggatct
     2761 tgcgaatgcc ctcgacagtg cgctcggcgc taccgacgat ctggcggcgc gcgttcgcgc
     2821 aattgaagtc gtcatcgcgg ggcggattat tttttcaacg agcctcggat tggaggatca
     2881 agttctcctc cacgccatcg tggcggcgaa ggctgaaatc gagtcgatca ccctcgacac
     2941 tggacggcat ttccccgaga cctacgacac tctgacgcgc actgaaagac tccttgggac
     3001 ccgcattcgc gtcgtcatgc cggatgccga ggacgtcgaa gaccttgtcg cccgggacgg
     3061 actattcggc tttcgatacg cagtcgagaa acggaaagct tgctgccacg cgcgcaaggt
     3121 gctcccgctc cggcgggcac tctccagcgc cgcgggctgg gttacaggcg tccggcgcga
     3181 acagtctgaa acgcgcgatg caacgccttt cgctacattc gacgcttcat tcaatctcat
     3241 caaggtcaat ccgatcgctg actggacgct cgatcgcgta gaagcctatg tcgccgaaca
     3301 tcgtgtgccg gtgaatgctc tgcacgcaca aggcttccct tccatcggct gccaaccttg
     3361 cacgcgggca attggcccag gagaaccaat ccgggccggg cgctggtggt gggaagacga
     3421 aactcgcaag gaatgcggat tgcacaaccg tcccgggttt gcaaagtaag tcgcgccgtt
     3481 gtctcatctc gtttttttga agccgagagc attttcatct tgct
//
LOCUS       DATMGX010001058         2601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_12582, whole genome shotgun sequence.
ACCESSION   DATMGX010001058 DATMGX010000000
VERSION     DATMGX010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12582"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VNR65_17380"
     CDS             <1..631
                     /locus_tag="VNR65_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWJ20492.1"
                     /translation="LGDSDALRVGQLVVALGNPFGLQASVTTGVVSALRRTLRATTGR
                     LIEDIIQTDAALNPGNSGGALLDSRGRVIGVNTAIIAGANATGFAVPINTATRVIPDL
                     MREGRVVRGYLGLAGQTVPFPKAAAARYGLSIPAGVQVVQIVPGGPAQKAGLRAGDVM
                     LSVDGKDAPSVDAIHRILDGNSIGREATLRVLRRGEVIEARITPSRRPE"
     gene            870..1016
                     /locus_tag="VNR65_17385"
     CDS             870..1016
                     /locus_tag="VNR65_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20493.1"
                     /translation="MRKFWRAFIADECPDEKERIRRERINNIMQNVVLLEAMRRWEME
                     SGRQ"
     gene            1013..1354
                     /locus_tag="VNR65_17390"
     CDS             1013..1354
                     /locus_tag="VNR65_17390"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HWJ20494.1"
                     /translation="MNVNQAALDNVSFALTNLQSCSLNRHAQNRIVGVCDGLRHRRRV
                     EAAVLFATFCPRFSRAERIAISLKATGIILFFALLGAAILSALGVTSPALQAAGGIIF
                     FIIALDMLRKL"
     gene            1472..>2601
                     /locus_tag="VNR65_17395"
     CDS             1472..>2601
                     /locus_tag="VNR65_17395"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWJ20495.1"
                     /translation="MGGFAEYDRYDAVGLAELVRKREVSAAELLAEARARCDRVNPKL
                     NAVVYRLDAEAERAASAVDKDRPFAGVPFLVKDLGPALAGAPLTCGSRLFANYVPEED
                     GEIIKRFKAAGLIAFGKTNVPEIGLVPFTEPELFGPCRNPWNLDRTPGGSSGGAASAV
                     AAGIVPMAHASDGGGSIRIPASCCGLVGLKTSRGLNPRDPQIETIISDFVVDHVISRS
                     VRDSAVALDAICNRPNAGFLSGLEAAPQPLKVAVVRSAMFGPSVAPEVRAALDSAVRL
                     MESLGHHIEDAEPQVDYAEFGMAFLLYWAIGAKQALEGAAELLGHSASREDLERTTWT
                     LASVGGAVPEAEKAWAKAVIERATRVFLEFSENYDVIMSPVLG"
BASE COUNT          482 a          748 c          814 g          557 t
ORIGIN      
        1 actcggcgat tccgacgcgc ttcgcgtggg ccagcttgtt gtcgcgctgg gaaatccatt
       61 cggccttcag gcatcggtca cgacaggcgt ggtgagcgcc ttgcggcgga ctctgcgcgc
      121 gacgactggc cgcctcatcg aagacatcat ccagaccgat gcagcgctca atcccggcaa
      181 ctcgggtggc gcgctgctgg atagccgggg ccgcgtcatc ggcgtgaaca ccgccattat
      241 tgcgggcgca aatgccaccg gcttcgctgt tccgatcaat acggcgacgc gggtcattcc
      301 cgaccttatg cgcgagggcc gcgtggtgcg tggctatctg ggccttgcgg ggcagaccgt
      361 tccgtttcca aaggcggccg ctgcgagata tgggctctct attcccgccg gcgtgcaagt
      421 cgttcagatc gtacctgggg ggcctgcgca aaaagccgga ctccgcgcag gcgatgtgat
      481 gttgtctgtc gatggaaagg atgcgccctc ggtcgacgcc attcatcgta tccttgacgg
      541 gaactcgatc ggacgagagg caacgcttcg tgttctgcgc cggggcgaag tcatcgaggc
      601 acgcattacg ccgtcgcgca ggcctgagta agcacccgtt aacgtgaggt ggcgggggtg
      661 tggtgttcga aacgcccctg cgcgcgcaaa actttccgcc ttttctgcct tcaagttgat
      721 aagatagaaa ctgatactct catattctgg ggatacacgg ggtaggcgat acaacccatc
      781 gcttatcaaa tattcccatc ctctccgccc tcatctcagg tggattatca atgaacgcaa
      841 tcaggcggcc tgttcaacgg aagcaagttt tgagaaagtt ctggcgcgct ttcatcgcgg
      901 atgagtgccc ggacgagaag gagcgcatcc gccgcgaacg gatcaacaac atcatgcaaa
      961 atgtggtttt gcttgaggcc atgcggcgat gggagatgga gtccgggagg cagtgaacgt
     1021 caaccaggcg gcgctggaca acgtctcctt cgcattgacg aatttgcagt cctgttcttt
     1081 aaatcggcat gctcaaaacc gcattgtggg cgtttgcgat ggtcttcgcc accgtcggcg
     1141 tgtagaggcc gcggttctct ttgcaacgtt ttgcccgcgc ttcagccgtg ctgaacgcat
     1201 tgcgatctca ctgaaagcaa cggggatcat cctcttcttc gcactgctgg gcgcagcaat
     1261 actttccgcg ctcggcgtca catccccggc tttgcaggct gcgggcggca tcatcttctt
     1321 cattattgcg ctcgatatgc tcagaaagtt gtgatgcgcg ttttcggcat cctcctcagc
     1381 gctatttccg ttcaatccgt gttcaacggg atttccgcga gtgggatttt tctgcgcacg
     1441 ctctaagtaa gaagtaggta ggagtatacg aatgggcggc tttgcggaat acgaccgcta
     1501 cgacgcggtc ggtttggcgg aacttgtgcg caaacgggag gtgagcgctg ccgagcttct
     1561 ggccgaagcg cgtgctcgtt gcgatcgcgt gaaccctaaa ctcaacgccg ttgtgtatcg
     1621 attggacgca gaggcggagc gagcggcaag cgcggtggat aaggatcggc ctttcgccgg
     1681 tgtccccttt ctggtcaaag accttggacc ggcactcgcg ggcgcaccgc ttacatgcgg
     1741 cagcaggctt ttcgcgaact acgtgcctga agaggacggc gaaatcataa agcgcttcaa
     1801 agctgcgggc ctgatcgctt ttggtaagac caatgttccc gagattgggc tcgtgccttt
     1861 tacggaaccc gaacttttcg gcccgtgccg caatccgtgg aacctcgatc ggacgccggg
     1921 cgggtcaagc ggtggggcgg cgtcggctgt cgcagccggc atcgtgccca tggcgcatgc
     1981 ctccgatggc gggggctcga tccgcattcc ggcgagctgc tgcggccttg tcggcttaaa
     2041 aacgtcgcgc gggctaaatc caagggatcc gcaaatcgaa accatcattt ccgacttcgt
     2101 cgtggatcac gtcataagcc gcagcgtccg cgactccgcc gtcgcgctgg acgccatctg
     2161 caaccgtccg aacgcgggct ttctgtccgg gctcgaagcc gccccacaac ctttgaaggt
     2221 ggcggttgtc cgaagcgcta tgttcggccc gtcggtggcg ccggaagtgc gggcggcgct
     2281 cgatagcgcg gtcaggctga tggagtcgct tggccatcat atcgaggacg ctgagcccca
     2341 agtcgattat gccgagttcg gcatggcttt ccttctttac tgggccatcg gtgcgaaaca
     2401 agcgcttgag ggcgccgcgg aactcctcgg gcattcggca tcacgcgagg acttggaacg
     2461 caccacatgg actctcgcga gcgtcggcgg cgctgtgccg gaagcggaga aggcttgggc
     2521 gaaggccgtc atcgaacgag cgaccagggt atttctggag ttttcggaaa actacgacgt
     2581 gatcatgagc cctgtcttgg g
//
LOCUS       DATMGX010001059         3274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_54078, whole genome shotgun sequence.
ACCESSION   DATMGX010001059 DATMGX010000000
VERSION     DATMGX010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54078"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..430)
                     /locus_tag="VNR65_17400"
     CDS             complement(98..430)
                     /locus_tag="VNR65_17400"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWJ20496.1"
                     /translation="MNTVEQLHQATKDFGDAATASAQTLATSIQTIASAHADYAKKAL
                     EHGSEFISQLTSVKEPSKLVELQSEYVKNAYETFVAEAKKISELYADFFKQTAKPLQD
                     LIAKKNAA"
     gene            complement(601..1800)
                     /locus_tag="VNR65_17405"
     CDS             complement(601..1800)
                     /locus_tag="VNR65_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HWJ20497.1"
                     /translation="MTDDMMNLRTLVEKTPDADLLREMIGFAAQRLMELEVEGQTGAA
                     YGEKNPERLAQRNGYRERIWETRAGAVELRIPKLRKGSYFPGFLEPRRMAEKALTAVV
                     QEAYVQGVSTRSVDDLVQAMGMTGISKSQVSRLCGEIDDKVKAFLARPIEGDWPYLWI
                     DATYVKVRQNGRIVSVAVIVAVGVNSDGRREVLGMDIGPSEAETFWTAFLRKLTRRGL
                     RGVKLVVSDAHEGIKATVAKVLNATWQRCRVHFMRNALAHAGKSGRRVVSAFIATAFA
                     QDDAEAATAQWRKVADQLRPKLPKLATFLDEAETDVLAYMTFPPQHRTKLHSTNPIER
                     LNGEIKRRTEVVGIFPNEDAIVRLVGAILLEQNDEWAVQRARYMTLETIAPLSDDPTV
                     SLPAIAS"
     gene            complement(2088..2576)
                     /locus_tag="VNR65_17410"
     CDS             complement(2088..2576)
                     /locus_tag="VNR65_17410"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HWJ20498.1"
                     /translation="MPTEIERKFLVANDGWKQSVIRCTRIRDGLIAVYFGRKARVRIL
                     GDRATIALKGQRSGLGRSEFEYAIPISDAEEMLRTMCDDRILEKVRHYVSYAGLTWEI
                     DVYEGLLKGVVIAEVELDRDDRLLELPDWVGKEITGDLRYSKFNMEKAAKRRKRLRQN
                     KS"
BASE COUNT          656 a          964 c          924 g          730 t
ORIGIN      
        1 ttgctgatgc cagcgcgata tggagggaat aaatgcccgg caaaggcttt tggattttcc
       61 aaatggatgt gagcgatcct gacagatatc gccacaatca agctgcgttc ttcttcgcaa
      121 tcaaatcttg taatggcttc gccgtctgct tgaagaagtc agcatagagt tcggagattt
      181 tcttcgcttc ggctacgaac gtttcataag cgttctttac gtattcgctc tgaagctcca
      241 ccaacttgct gggctcttta acgctggtaa gctgagaaat gaactccgaa ccatgttcca
      301 atgccttctt ggcgtagtcc gcatgggcgc tcgcaattgt ctggatggaa gttgcaaggg
      361 tttgtgccga tgccgttgcc gcatcgccga agtctttagt agcttgatga agttgttcta
      421 cggtgttcat tttgcatttc ctatgagtga aagcccacgc cttgatgctg cactcaacat
      481 gggcgcaagc aatcgtttag attgtgattt ttatataact gacaggtcga accgatcgtg
      541 tcccccggcg tggtgtagct ggcggtgggg caccgcgttc gccggcaaga gccgggcagg
      601 tcagctggca atcgccggaa ggctgacagt tggatcatcg ctcaacggcg cgatggtttc
      661 cagcgtcatg tagcgggcac gctggacggc ccattcatcg ttctgttcga gcaggatcgc
      721 gccgacgaga cggacgatgg cgtcctcgtt ggggaagatg ccgaccacct cggtccggcg
      781 tttgatctcg ccgttgagac gctcgatcgg gttggtcgag tgcagcttgg tccgatgctg
      841 cggcgggaac gtcatgtagg cgagcacgtc ggtctcggcc tcgtccagga aggtggcgag
      901 cttcggcagc ttggggcgca gctggtcggc gaccttgcgc cactgcgctg ttgcggcctc
      961 ggcatcgtcc tgagcgaacg ctgtggcaat gaaggctgag acgacgcgcc ggccgctctt
     1021 gccggcatgc gccagcgcgt tgcgcatgaa gtgcacgcgg cagcgctgcc aggtggcgtt
     1081 gagcaccttg gccacggtgg ccttgatgcc ctcgtgggcg tccgagacga caagcttgac
     1141 gccgcgcagg ccgcggcggg tgagcttgcg caggaacgcc gtccaaaagg tctcggcctc
     1201 ggaggggcca atatccatac cgagaacctc gcgccgcccg tcactgttga cgccgaccgc
     1261 gacgatcacc gcgaccgata cgatgcggcc attctggcgc accttcacat aggtggcatc
     1321 gatccacaga tacggccagt cgccctcgat cggacgggct aggaaggcct tcaccttgtc
     1381 gtcgatctcg ccgcacagcc ggctgacctg gctcttggag atgccggtca tgcccatcgc
     1441 ctgcaccaga tcgtccaccg aacgggtcga aacgccctgc acgtaggctt cctgcaccac
     1501 ggcggtgagc gccttctccg ccatccggcg cggctccagg aaaccgggga agtaagaacc
     1561 cttgcgcagc tttggaatgc gcagttcgac cgcgccggcg cgggtctccc agatccgctc
     1621 ccggtagccg ttgcgctggg ccagacgctc ggggttcttc tcgccatagg ccgccccggt
     1681 ctggccttcg acttccagct ccatcagacg ctgggcggca aagccgatca tctcgcgcag
     1741 cagatcggcg tctggggtct tctctacgag cgtgcgcagg ttcatcatat cgtcggtcat
     1801 cggtggttcc tcggttgcgt tggcgtgtcg caacccgatc ctaccggaga actgccggtg
     1861 accaccgcaa agccgcccgc ccgctacggc gctatttgag gcgcgcgtcc gggcggcttt
     1921 gctctaccga gctacaccac ctccggggac acgaccggtc gaacctgtcg gttctgtcgt
     1981 aagtaaaact agtggcatgc agttatgata gccatgtgac ggtcggtaga gaaaccttga
     2041 gaccccgacc gcagccttgc agcgggcaat tgagcgctat ggtcgtgtca agatttgttc
     2101 tggcgcagcc tctttcggcg cttagctgct ttctccatat tgaatttgct ataccgcaag
     2161 tcgcccgtta tttcttttcc aacccaatcg ggcaattcca ataggcggtc atctcgatct
     2221 aattcgactt cggcaattac gacgcctttc agaagacctt cgtagacgtc aatttcccaa
     2281 gtcagccccg catacgaaac gtaatgtcga accttctcca aaatgcggtc atcacacatc
     2341 gttcgcaaca tttcttctgc atcggaaatt ggtatcgcat attcaaactc ggagcgacct
     2401 aagccgctgc gctgaccttt aagtgcgatc gttgcccgat cacccaaaat tcgcacccga
     2461 gccttacgac caaaataaac cgcaatgaga ccatctcgta ttcgagtaca tcgaatgaca
     2521 gattgtttcc atccgtcgtt agcaaccaaa aatttccgct cgatttctgt cggcatgagg
     2581 gagtttgaat tcaactcagg cgaactacgc tccaaatctg agaccgagta aacctagaag
     2641 taggttgatt gggaatgcac gtggcatttc gccaaccgca tcaacgcaag cggacgccct
     2701 cgggtgtgga aaaacaaccc gaaagcgccc gcatattgca ttgaagaatg ctcggcttgt
     2761 cctaagagag acttccccaa tgttttaata acgaaactcg aatccgcagc cgtgtctttg
     2821 acttagatca aatagtcgat cgattggtct ggtagctggt aataactgga tattgcttag
     2881 ttcccccaag gagcatgtcc agccaagaat gaccccagcg gcgcccaccc tggggtcatt
     2941 tctttgctta catctctctc cgagctcacc gttgcgagac gagcggcagc aatcagcgca
     3001 tcgatggtgg tcagcagaga agggcttatt acaggtcacc gccactctat ccgcaccaca
     3061 cccacaatgg ctgactaaaa accgaagcgt tgccttgctg tgggggattt gtgcggtatc
     3121 ctcgaagcct gcgaccgcga tccgttcctg gccgtgggct cgctgatcga aagcggtcat
     3181 gccatgccgg cggaaacccg cagcgccttg gccatgggcc tggcgcttgc tggaatcccc
     3241 gaagcgcgcg ccgccgcggt tctgttcctg ctcg
//
LOCUS       DATMGX010001060         2752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_58519, whole genome shotgun sequence.
ACCESSION   DATMGX010001060 DATMGX010000000
VERSION     DATMGX010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58519"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..788
                     /locus_tag="VNR65_17415"
     CDS             357..788
                     /locus_tag="VNR65_17415"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HWJ20499.1"
                     /translation="MDELVRSLKALHTSLIDALQGYKEGLKDAHGEGLAPLFAEMIAI
                     HGKDADAISEQLKRLGTDTDDRGSLMGTFDRTVMKISSLFTKLDEKFLPSLIDGEKRV
                     LDHYDRAINASSPGNAEYPVLVKQREALRAKVADMGAGKPT"
     gene            complement(1114..1521)
                     /gene="apaG"
                     /locus_tag="VNR65_17420"
     CDS             complement(1114..1521)
                     /gene="apaG"
                     /locus_tag="VNR65_17420"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HWJ20500.1"
                     /translation="MAARAKYSATTRQISVSVHPTYLDDQSAPEQSHFVWAYHIVIEN
                     RGTETVQLRSRHWRITNARGELHEVRGPGVVGEQPRLEPGDAFEYISHVPLTTPSGIM
                     TGSYQMETRDGESFDVEIPAFSLDSPHQSVKLN"
     gene            complement(1541..2404)
                     /locus_tag="VNR65_17425"
     CDS             complement(1541..2404)
                     /locus_tag="VNR65_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20501.1"
                     /translation="MTHEAAPKKKRHAKDTLANDDHPASAADNPLRCKMEIGMLGGIA
                     GVVAGWLSGYAFRSWKYKRERKRGLKNEFHDLITVKAAIVWRDDEGDKSWHLSIRDVQ
                     AKVPMLHVFANRPITDVVRAASDETKEGLVRVSNPVIHRRMMDLIRRHFTGNDLAAVY
                     KFLTGRHVRRDRIIFGLLSPHHKPADGDAELFRLGMASILLIHEERLDILCRPDFLDF
                     IELGASATRTDAELVHKIALSAPEELQKKPGLAALGIADVESEDEEHLADQVAQKVLA
                     QLATKRLVDAR"
     gene            complement(2486..>2752)
                     /locus_tag="VNR65_17430"
     CDS             complement(2486..>2752)
                     /locus_tag="VNR65_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HWJ20502.1"
                     /translation="AATALSVSAYLRNLGLGFQPQSTLDHEAVLALLKVNADQGRLGG
                     LFKLWLSGQSAPSAEVAEIRKLLAAIEESQVKLKALIGQLSDWT"
BASE COUNT          552 a          786 c          805 g          609 t
ORIGIN      
        1 gcctgcgata gcggatttcc gaaatgcgca attgatcgtt cgtggacagc tcgaccgctt
       61 tgtccatcga gatcctttgg tgagaagcga tcaagtcgaa ccgctaaggt ttaggcgctg
      121 cgaccgtgtt tacatttaac cggctgagcg taaagagtcg gaaaatactc aaacggagct
      181 tggtcctgtt gcgctttggg atcgattgcc agaagttctc gatcatttgt aagttattgt
      241 cgtcattgca cgctgcaata aatgtcagac gtcacgcacg tgaaaggctt atgctagagt
      301 ttcgaggtgc tgggcgtcct tggcgtccgg ccgacaaaaa gcttaggagg acgaccatgg
      361 acgaactcgt tcgcagcctc aaggctctgc acacaagctt gatcgacgcc cttcaaggat
      421 acaaagaggg gctcaaggat gcgcatggcg agggccttgc gccgttgttc gctgaaatga
      481 tcgccatcca cgggaaggac gcggacgcca tttccgaaca gctgaagcgc ctgggcaccg
      541 acaccgacga tcgcgggtcg ctcatgggca ccttcgatcg cacggtgatg aagatttcct
      601 ctctattcac gaagctggac gagaaatttc ttccgagcct aatcgacggc gagaaacgag
      661 tactcgacca ttacgaccgt gccatcaacg cgtcctcgcc gggcaatgcc gagtatccgg
      721 tgcttgtgaa gcaacgggag gctctcaggg caaaggtcgc cgacatgggg gcgggcaagc
      781 cgacctgagg tgtttgcgcg gctgtcgaag tggacggagg gaggcgcctg gtgtaggtcg
      841 ctcgtatcga tgacccggct tggcttgggc gaaagccata tcgcgcaggt ggcggcgtac
      901 gatcgagcgc ctggatccca gacgatgacg ctgtctcatg gccggagctt ggcacaggac
      961 cgtcgcggct gaaaagtctt ccttgcggtc atccccttaa cacccccgac gtctactcgc
     1021 ctgaccgcct gatcgttggt aatagtcagg tttcgagggt agagcctcca ggaaagattt
     1081 accggtttcg accctcttgg gacgagcttc ttttcaatta agcttcactg actgatgagg
     1141 gctgtccagg gagaaggcgg ggatctcgac gtcgaagctt tcgccgtccc gggtttccat
     1201 ttgatagctg cccgtcatga tgcccgaggg cgtcgttagc ggcacgtggc tgatgtattc
     1261 aaacgcatcg ccgggctcaa ggcgcggctg ctcgccgacc acacccggac cgcgtacctc
     1321 gtgtagttct ccacgcgcgt tggtgatgcg ccaatggcgg cttcgcaact gcactgtttc
     1381 ggtgccacga ttttcgatca cgatgtgata ggcccagacg aaatggcttt gctccggcgc
     1441 cgattggtcg tcgagatagg tagggtggac gctcactgaa atctgtcgcg tcgtcgcgct
     1501 gtatttggcc ctggccgcca tgccggtctc cgatttggct ctaccttgcg tcaaccaacc
     1561 tcttcgttgc aagttgtgct aagacctttt gtgcgacttg gtcagcgagg tgttcctcgt
     1621 cctcgctctc aacgtccgca atcccaagcg cagccaagcc cggttttttc tgcagctctt
     1681 caggcgcaga gagcgctatt ttgtgaacca gttcagcatc ggtccgagtc gccgacgcac
     1741 caagctcgat gaaatccagg aagtcgggac ggcaaaggat gtcgagccgc tcttcgtgga
     1801 taagcaggat gctggccata cccaagcgaa agagttccgc atcgccgtcc gccggtttgt
     1861 gatgaggaga gagcaaaccg aagatgattc tatccctccg cacgtgccgg ccggtgagga
     1921 acttgtagac cgccgccagg tcattcccgg taaaatgacg gcggataagg tccatcatcc
     1981 gccggtgaat gaccgggttg gaaacacgca cgagcccttc tttcgtctcg tcgctcgcag
     2041 cacgaacgac atccgtaata ggcctgtttg caaagacgtg gagcatgggt acttttgcct
     2101 gcacgtcgcg aatcgagaga tgccagctct tgtcgccctc gtcatcacgc cagacaatcg
     2161 cggccttgac ggtgatgaga tcgtgaaatt catttttcag gccgcgtttt cgctctctct
     2221 tatatttcca cgacctaaat gcatagcccg acagccaacc tgccaccacg cctgcgatgc
     2281 cgcccagcat tccaatttcc atcttacacc tcaggggatt atcggccgca ctggccgggt
     2341 gatcatcgtt ggcaagcgtg tccttcgcgt gacgcttctt cttcggggcc gcttcatggg
     2401 tcatgggccg tgggcgcagg cttctgatta gccgattgtg gggcctgtca ggagcgtcgt
     2461 cctaggtcta accctagtgt tagtcctagg tccagtcgga caattgcccg atcagcgcct
     2521 tgagtttcac ctggctctcc tcgatagcgg cgagcagctt ccggatttcc gcgacctccg
     2581 ctgacggcgc gctttgcccc gacagccaga gcttgaacaa gccgcccagc cgaccctgat
     2641 cggcgttcac cttaagaagg gcgagaacgg cctcgtgatc caaggtcgat tgcggctgaa
     2701 acccaagccc gagattgcgc agataggcgg ataccgaaag ggcggtggca gc
//
LOCUS       DATMGX010001061         3060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_142601, whole genome shotgun sequence.
ACCESSION   DATMGX010001061 DATMGX010000000
VERSION     DATMGX010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142601"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VNR65_17435"
     CDS             complement(<1..400)
                     /locus_tag="VNR65_17435"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWJ20503.1"
                     /translation="MSLHSETRRITAPDIKARKGQQKIVSLTAYHAHTARLIDPHVDF
                     LLVGDSLGMVMHGYETTVPVPLELMIMHGAAVVRGSRRALVIVDMPFGSYEESPPVAF
                     RNAVRIMKETGCGAVKLEGGRRMAETITYLM"
     gene            583..1548
                     /locus_tag="VNR65_17440"
     CDS             583..1548
                     /locus_tag="VNR65_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20504.1"
                     /translation="MKRRFVVAFLFAAVAIAMSARVGCLSAAPFSNAAFSLSSPKTSA
                     LTRVQSACGWTYSCTDLGKAGNVQIYGPVNIYTDAKPEIWRVPEGAEPWTGDQGDQQW
                     RGWGCSGHACDERCGAFCWFNRVRHGYCGHGCDAYREHVMFQPMGGTLRPFIYQNQSD
                     GDCGYGPSGGYGPNRCGRAPVAYNPGQDGYSQTSSGYYWRNNGSAQSYYSRNNGSGQG
                     YYPPNGGSAQDGYYPANNGGGAADYPQNGSYDSQGPQGERFIDRLRRYIYGNPRREVY
                     GDNGAVHGNGAVRFERPSNDLVPLRRFNGPKYPPNSTGTESQRPK"
     gene            complement(1573..1758)
                     /locus_tag="VNR65_17445"
     CDS             complement(1573..1758)
                     /locus_tag="VNR65_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20505.1"
                     /translation="MAKVVSNDQAGEKELSELISRLRSVAEAMRNLSGDRAGLRERLQ
                     ELKDEVNWVSSRLRRFS"
     gene            2057..>3060
                     /locus_tag="VNR65_17450"
     CDS             2057..>3060
                     /locus_tag="VNR65_17450"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HWJ20506.1"
                     /translation="MLHRRNFLKLALAAQAFAALPANAAASNLRFGEPQPFSSDWLKA
                     HAQSLAAAPYSPPPRPDPSLVASIDYDLHGKLKYKNDFALYGDGHPSAFPITFMHVGQ
                     YFPKTVRLYAVEPSSDGTSSAREILYDPDYFSIPADSVAAKLPQNPSRFAGFWLREPK
                     AGTEDWRIAEPWVTFLGASYFRAKGELGQVGLSARGIALTPGGNAIEEFPDFVAFWFL
                     PAAPAARSVTVYALLDGPSVTGAYKFVMERTKGVVMEIENTLFFRQPVERLGLAPLTS
                     MYWYSESVKQPGIDWRPEVHDSDGLAMWTGGGEHIWRPLNNPAHITISTFADERPRGF
                     G"
BASE COUNT          623 a          920 c          870 g          647 t
ORIGIN      
        1 ccatcagata tgtaatcgtc tccgccatgc gccggccgcc ttcgagcttg accgcgccgc
       61 agcccgtttc tttcattatg cggacggcat tccgaaaggc gacggggggc gactcctcat
      121 aggagccgaa aggcatatcc acaatcacga gggcacgcct ggacccacgg accaccgccg
      181 cgccatgcat gatcatgagt tccaggggaa ccggcaccgt cgtctcatag ccgtgcatca
      241 ccatcccgag cgaatcgccg accaggagga aatcaacatg gggatcgatc aagcgggccg
      301 tgtgcgcgtg atacgccgtc agcgacacga ttttctgctg ccccttccga gccttgatat
      361 ctggcgccgt gatccggcgc gtttcggaat gcagtgacat gagaaggtcc cccgagatga
      421 tgctcgtcaa tagcgaaatt gatcacggct ggaaataggc gcgacgagca aagtcgcaat
      481 taccaatcga aaattaaccg agatcccatt cggaggcggc cggtagttca aagtttaaga
      541 aaatcgacct attgtcgaag gatggtaagg tcggggcttt ttatgaaaag gcgctttgtc
      601 gtcgcgttct tgttcgccgc tgtcgcgatt gcgatgagcg cgcgcgttgg ctgtttatct
      661 gcagcgccat tttccaacgc ggcattttcg ctttcgtctc caaaaacatc ggcgttgacg
      721 agggttcagt ctgcgtgcgg ctggacttat tcctgcactg accttgggaa agcgggcaat
      781 gtccaaatct acggcccggt gaatatctac acagacgcca aaccggaaat atggagagtc
      841 cctgaagggg cggagccctg gactggagac caaggagatc agcagtggcg gggctggggc
      901 tgcagcggcc acgcgtgcga cgaaaggtgc ggagccttct gctggttcaa ccgcgtccgg
      961 cacggttatt gcggccacgg gtgcgacgcc taccgcgagc atgtcatgtt tcagcccatg
     1021 ggcggaactc tgaggccctt catctatcag aaccagtcgg atggggattg tggctacggt
     1081 ccgtcaggcg gttatggacc gaacaggtgc ggccgggcgc ctgttgccta taacccaggt
     1141 caggacggct acagccaaac ttcgtcgggc tactattggc ggaacaatgg ctcggcccaa
     1201 agctactatt cccggaacaa tggctcgggc caaggctact atccgccgaa cggtggctcg
     1261 gcccaagacg ggtactatcc ggcaaacaat ggcggaggag ctgccgatta tccccagaac
     1321 ggatcctacg attctcaggg tccccaaggc gagcgcttca ttgatcgtct ccggcgctat
     1381 atttacggta accctcgtcg tgaagtttac ggcgacaacg gcgccgtcca tggcaacggg
     1441 gccgtccgct ttgagaggcc aagcaatgac ctcgtgcctc tccgccgttt caacgggccg
     1501 aaataccccc cgaattccac cggcacagaa tcccagcgcc ccaaataacc aatcgctatt
     1561 cgccgtaaag cctcaagaga agcgccgcag gcgggaactg acccaattca cctcatcctt
     1621 gagttcttgc agcctttccc gaagacccgc ccgatcgccc gagaggttcc tcatggcctc
     1681 agccacgctg cgaaggcgac taatcaactc cgaaagttct ttttctccag cctgatcgtt
     1741 gcttacgacc tttgccatag ccctctccat cccagtgccc cctggtaggg aagagtatag
     1801 ataagcaact caagcccgcg gcataaattt tcgtaatgtt tgtagtaatc cccatcttgg
     1861 cggtgccttg ctaagcaagc ctttttttgg tttgccttga tacgagagca gtccctcaag
     1921 cgttggcttc tgccaacgga tgataaatta acaattgccc cttcccattt tctcactgca
     1981 ccgctagaga cagtcccggc aaacgcgggg cggctgaccc gcgggacctt gcagctttat
     2041 tgaaggcact ccgatcatgc tccatcgccg taatttcctg aaactggcgc tggccgcaca
     2101 ggcattcgcg gctttacctg cgaatgcggc ggcttcaaat ctccgtttcg gggaacccca
     2161 gcctttttca tccgattggt tgaaggcgca tgcgcaatct cttgcggcgg cgccgtacag
     2221 ccccccgccg cgccctgatc cgagccttgt tgcatcgatc gattacgatt tgcatggcaa
     2281 actgaaatac aaaaatgatt tcgcgctgta tggcgacggc catcccagtg ccttcccgat
     2341 caccttcatg catgttgggc aatattttcc caagaccgtg cgcctgtacg ccgtggaacc
     2401 gtccagcgat ggcaccagca gcgcgaggga aatcctttac gacccggatt acttctcaat
     2461 tcccgccgat agtgttgcgg cgaaacttcc ccaaaatccc tcgcgttttg cgggcttctg
     2521 gctgcgcgag ccgaaggccg gcaccgagga ctggcggatc gcggaaccct gggtgacgtt
     2581 ccttggcgca tcctattttc gcgcaaaagg cgaattggga caggtgggtt tgtccgcgcg
     2641 ggggatcgcc ctcacgccag gcggcaatgc gatcgaggaa tttccggact tcgtcgcctt
     2701 ctggttcctg ccggcagccc cggccgcacg gagcgtcacg gtttatgccc tgctcgacgg
     2761 gccgagcgtc actggcgcct acaagttcgt catggagcgg accaagggcg tggtcatgga
     2821 gatcgagaat acgctgttct tccgccagcc ggtggagcgg cttgggcttg cgccgcttac
     2881 ctcgatgtac tggtattcgg aatctgtgaa gcagccgggc atcgactggc gtccggaggt
     2941 gcatgattcg gacgggcttg cgatgtggac cggcggcggc gagcacatct ggcgtccgct
     3001 caacaatccc gcgcacatca ctatttcgac cttcgccgac gagcgcccgc gcggttttgg
//
LOCUS       DATMGX010001062         2888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_475439, whole genome shotgun sequence.
ACCESSION   DATMGX010001062 DATMGX010000000
VERSION     DATMGX010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475439"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /gene="pnp"
                     /locus_tag="VNR65_17455"
     CDS             <1..1076
                     /gene="pnp"
                     /locus_tag="VNR65_17455"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWJ20507.1"
                     /translation="GTGEDEQWIDSLEGTYKETFLLHYNFPPFSVGEVGRMGSPGRRE
                     IGHGKLAWRAVHPMIPNSETFPYTIRVVSEITESNGSSSMASVCGASLAMMDAGIPLK
                     APVAGIAMGLIKESDGVAVLSDIIGDEDHLGDMDFKVAGTAQGVTALQMDIKITGITT
                     EIMKTALEQAHDGRLHILGEMSKALGHARTDLGEHAPRIETITIPTDKIREVIGSGGS
                     VIRSIVEESGAKIDISDDGTVKIAAANLNSIKDAINRIKMITSEPEVGLIYKGKVVKI
                     VEFGAFVNFFGSRDGLVHISQMSDRRLKHASEVLKEGQEVYVKCMGFDDRGKVRLAMK
                     GIDQKTGKEVAAPEQPATEPAAS"
     gene            1898..>2888
                     /locus_tag="VNR65_17460"
     CDS             1898..>2888
                     /locus_tag="VNR65_17460"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWJ20508.1"
                     /translation="MGLLVDGVWQDQWYDTAKSGGRFIRSESQFRNWITPDGSAGPSG
                     VGSFAAEPDRYHLYVSLACPWAHRTLIFRALKGLESFIPISVVNWLMGPQGWTFDDGP
                     GVIPDTVNGAKRLFEIYTKAKPSYSGRVNVPTLWDKKTGGIVNNESSEILRMFNNAFD
                     GVGAKAGDYYPLDLRPAIDSLNAEIYARVNNGVYRAGFATTQEAYEEAVRPLFEALDK
                     LEARLSHQRYLCGERITEADWRLFTTLIRFDAVYHGHFKCNIRRIADYPNLWGYTREL
                     YHWPGVRETTNFQHIKHHYYETHKTINPTGIVPVGPDLDFDAPHGRDHFSVKST"
BASE COUNT          634 a          844 c          823 g          587 t
ORIGIN      
        1 tcggcacggg cgaggacgag caatggatcg actcgctgga gggcacctat aaggagacct
       61 tcctgctgca ctataacttc ccgcccttct cggtcggtga ggtgggccgc atgggttctc
      121 ctggacgccg cgagatcggc catggcaagc ttgcctggcg cgcggtccat cccatgatcc
      181 cgaattccga aaccttcccc tacaccataa gggtcgtttc ggagatcacg gaatccaatg
      241 gatcgtcatc catggcgagc gtctgcggcg cttcgctggc gatgatggac gcgggcattc
      301 cgttgaaagc gcctgttgca ggcatcgcca tgggcctcat caaggaatcg gatggcgtcg
      361 cggttttgtc cgacatcatc ggcgacgagg atcatctcgg cgacatggac ttcaaggttg
      421 ccggcacggc tcagggcgtg actgcgctcc agatggacat caagatcacc ggcatcacga
      481 ccgagatcat gaaaaccgct ttggagcaag cgcacgacgg ccggttgcac attctcggag
      541 agatgtcgaa agcgcttggc cacgctcgca ccgatcttgg tgagcatgcg ccgcgcatcg
      601 agacgattac catcccgacc gacaagatcc gcgaagtgat cgggtcgggc ggttccgtga
      661 tccgctccat cgtcgaggag tcgggcgcaa agatcgacat ctccgacgac ggcaccgtga
      721 agatcgccgc cgccaacctg aattcgatca aggacgccat caaccggatc aagatgatca
      781 catccgaacc cgaggttggg ctgatctaca agggcaaggt cgtgaagatc gtcgagttcg
      841 gcgcctttgt gaacttcttc gggtcacgcg atggcctcgt ccacatctcg cagatgtcgg
      901 atcgcaggct gaagcatgct tccgaggtct tgaaggaagg ccaggaggtc tatgtgaaat
      961 gcatgggctt cgacgatcgc gggaaggtcc ggctcgcgat gaagggcatc gaccagaaga
     1021 cgggcaagga agtggcagcg ccggagcaac ccgctacgga gccggccgcg agctaatttg
     1081 ggttcgtcat cccggaacgc cgaaggcgta tccgggatcg tcccttggcc cggcgtccag
     1141 acacattacc cggataagcg ccaaagcgct ttccgggact tgatgacccc gctatcacag
     1201 ccttgtaagc ttacagttcg gctttttcga actgcaagcc agaagccacg gtccctaagc
     1261 cacgatcatg gtgaatgctt cgttgccgcg ccggaaagcc gttaacgaaa gcgtggttaa
     1321 tttcggcgcg cgggatttat ttctggtcca aaaacgcttt attaccctta acgtcgagtt
     1381 tacactgcgc aggacgaggc tcgtcgcgcc tacgacaggc ggtcctaacg cgttgaaggt
     1441 gatcgggttt ttttcggcag ccgcccccat taaatagaat ttaatgccgg aattttccca
     1501 ttaaatgggc gcgcaaatgt tcgttaaata tgttttggcc gcgcatgtta accatgaaca
     1561 cgcctcttgc ccgagtcgac ggcgccatcc cttcctcaat tttctgtttt aaaacagcat
     1621 cttatcgacc ttaacgtctc ggtaaagcgc cctctcctat catatcaggc gatgcccaag
     1681 cggtgcccag aatctcaagc gagatgccgc catctgcaaa agagcataca gaaagccagt
     1741 tgagctgtaa gccgggtcca gtgcggggga agcggacgga aacaggggta attaagttcc
     1801 gggatggctg tgcggtctgc tcaccaaact tttattggcc gcagatcacc aaagcccaat
     1861 ctcgaaggca agctcttgag catgcgtgga ggttggaatg ggtcttctgg tcgatggtgt
     1921 ctggcaagac cagtggtacg ataccgcaaa gtcaggagga cgcttcatcc gctctgaaag
     1981 ccagttccgc aactggatca ccccggacgg gagcgcgggg ccttcgggcg ttggcagctt
     2041 cgcagccgaa cccgatcgtt atcaccttta tgtctctctt gcttgcccgt gggcgcatag
     2101 gacgctgata tttcgcgcgc ttaaagggct cgagagcttc attcccatct ccgttgtgaa
     2161 ttggctcatg gggccgcaag gctggacctt cgacgacggc cccggcgtca tccccgacac
     2221 cgtcaacggg gccaagcgcc tttttgaaat ctacacgaag gcgaaaccgt cctattcggg
     2281 gcgcgtgaac gtcccgaccc tgtgggacaa gaaaaccggc ggaatcgtga acaacgagtc
     2341 gtccgaaatc ctccgcatgt tcaacaatgc gttcgacggc gtcggagcga aggcgggcga
     2401 ctattaccct ctggacttac ggcccgcgat tgatagcctc aacgcggaga tctacgcccg
     2461 cgtcaacaac ggcgtctacc gtgcggggtt cgcaacgaca caagaggcct acgaagaggc
     2521 agtgcgcccg cttttcgagg cgctcgacaa gcttgaagcg cgcctctccc accaacgata
     2581 tctatgcggc gagcgcatca ccgaagctga ctggcgtctg ttcacaacgc tgatccggtt
     2641 cgatgccgtc tatcacgggc acttcaagtg caatatcagg cgcatcgccg actatccgaa
     2701 cttgtggggc tatacacgtg agctttatca ctggcctggc gtgcgcgaga cgacaaactt
     2761 ccagcatatc aagcaccact attacgaaac ccacaagacc atcaatccaa ccgggattgt
     2821 tcccgtcggc cccgaccttg atttcgacgc cccgcacgga cgggatcact tttcggtgaa
     2881 aagcaccc
//
LOCUS       DATMGX010001063         3213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_12618, whole genome shotgun sequence.
ACCESSION   DATMGX010001063 DATMGX010000000
VERSION     DATMGX010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12618"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VNR65_17465"
     CDS             complement(<1..254)
                     /locus_tag="VNR65_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20509.1"
                     /translation="MLEFIFLGFFFLTILVFALAYWPKKAKTRAPAPGATDGAPPKGA
                     PARAVQTAPSSRRVALVIGNGAYTSVDRLKNPANDASAVAG"
     gene            complement(1093..2400)
                     /locus_tag="VNR65_17470"
     CDS             complement(1093..2400)
                     /locus_tag="VNR65_17470"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWJ20510.1"
                     /translation="MPWPQLFVLILLIAVNGFLVMAELAIVSARPVRLQMLVNDGHPG
                     AMAAMALNADSARFLPSVQIGITVVAIMSGAYGEHTLSPALAEALAGLSWLSAYAETI
                     ASTIVVCSIAYFSLVIGELVPKRIALANRERIACSVAPAMTVLATVATPISTLLRWST
                     DAVLHLVSPAVQAEPDVTEEEVKSMIAEGTEAGIFEKGEQEIIERLLRVADRSVRSIM
                     VPRPDIVWLDITESESCIYNDIAASGHSRFPVCRGDVDETIGIVHAKTLLDQLHKSGK
                     INLEAAIKTPLYVHESTNVLKLLEMFKKFLVHMAIVLDEHGTVQGIVTPTDILTGIAG
                     DLPEEEADIEPSAVQREDGSWLMDGQMPVFEVERVLSLTGLAIDDAEYTTLAGFVLSQ
                     LGRIPATGETFRWKNWRFEVVDLDGRRIDKVLATHIPNGTNGV"
BASE COUNT          718 a          884 c          933 g          678 t
ORIGIN      
        1 ccagccacgg cagatgcgtc attagcaggg ttctttagcc gatcgacaga agtataggcg
       61 ccgttgccga tgacaagtgc cacccggcgc gacgatggag cggtctggac cgctcgggcc
      121 ggtgccccct tgggcggcgc accgtcggtc gcgcccggcg cgggagcgcg cgtcttcgct
      181 ttctttggcc agtatgccaa agcaaaaacc aagatcgtta aaaagaagaa tcctagaaag
      241 ataaattcca gcatgcccgc ttcatctttt gttttgtaac gctctatcgc cagacaggtt
      301 tatattcgct taaccgatgc acaagctcgt ggttcttgtg cgtattatgc gggatggcgc
      361 gatttccgcg tggcgtagag ccgcagctgg gtggggtttg cggcggttga ttcagcggcg
      421 cgccacaggc ggtgtcaaga gcgccatcct tgccatcggc atcttggacg gcgcccaatc
      481 gcaagcctca ggtaaaccgt tcgtcgcctc gccggtcatt aattagaaca tggttaccag
      541 gagcgcgccg gatttattcc cggccatgtg tttggttaaa aagtctttaa tccggcgtga
      601 aacgacgagc gtcgcgcctt agggctggat gtaagccgtc taccgtaagt tactgaaaaa
      661 aatcaatctt ttatccttaa caaagcgccc attaaataga atttaatgcc agaattttct
      721 cattaactgg gacgagcaaa agtccgttaa atatattgcg agttcgcaaa ttaacgcttg
      781 ggcgcagccg gaacagctgc aacgcaggct gcggcgatgg cgccccgttc ggtattttcc
      841 tcgcaattag ctccctatta tcaagggttg gagctggctc acgctctcgt taacctgccg
      901 tgaaagcgtt ttcggtgagg atctttcaga agaaggaatg cggacgcacg tggcggggag
      961 aaattgacaa acagatgttg ccattcgcca gatgcataca aaaaaacacc ggacgcgcac
     1021 gtcgaagcaa gcgcgcgata ccctgatgcg tgtccttcca ggcaaatccg caagcgcagc
     1081 ctcgcgggac tgctaaactc cgttcgtccc attcggaatg tgcgtcgcaa gcaccttatc
     1141 gatcctccgc ccatcgaggt cgacaacctc gaaccgccag ttcttccagc ggaatgtttc
     1201 gccggtggcg ggaatccggc ccagctgcga aagcacgaag ccggccagcg tggtgtattc
     1261 cgcatcgtcg atggcgagcc ctgtcaagct cagaacgcgc tcgacctcga acacgggcat
     1321 ttgcccgtcc atgagccagg agccgtcctc gcgctgcacg gcgctcggct cgatgtcggc
     1381 ctcctcctcc ggcaaatcgc ccgcgatgcc cgtcagaatg tcggtcggcg tgacgatccc
     1441 ttgaactgtt ccatgctcgt cgagcacgat cgccatgtgc accaggaact tcttgaacat
     1501 ctcgagcagc ttgaggacat tcgtgctctc gtgcacgtaa aggggcgtct tgatcgcggc
     1561 ttccaggttg atctttccgc ttttgtggag ttgatcaagc aacgtcttcg cgtgcacgat
     1621 gccgatggtt tcatccacgt cgccgcggca taccggaaac cgcgaatgcc cgctcgcggc
     1681 gatatcgttg tagatgcagg attcgctttc tgtgatgtcg agccagacga tgtcgggcct
     1741 cggcaccatg atcgagcgga cggaccggtc cgccacacgg aggaggcgct cgatgatctc
     1801 ctgttcaccc ttttcgaaga tgccggcctc ggtgccctcg gcgatcatcg atttaacttc
     1861 ctcctcggta acgtcaggct ccgcttgaac agcggggcta acgaggtgaa ggacggcatc
     1921 cgtagaccag cgcaggagag tcgagatcgg tgtcgcgacg gtggccagca cggtcatggc
     1981 cggggccacg gagcaagcga tgcgctcgcg gttggcaagg gcgatgcgtt tcgggacaag
     2041 ctcgccgatc acgagcgaga aataggcaat gctgcagacc acgattgtgg acgcgatcgt
     2101 ctcggcatag gcgctcagcc aggacaagcc tgcaagggct tcagccaggg cgggagacag
     2161 cgtgtgctct ccataggcgc ccgacatgat tgccacaact gtgatgccga tctgaaccga
     2221 gggaagaaag cgggctgaat ctgcgttcag cgccattgct gccatcgcac ccgggtgccc
     2281 gtcattgacg agcatttgca accggactgg cctagccgaa acaatagcca gttctgccat
     2341 gacgaggaaa ccgttcacgg cgatcaggag gataaggacg aaaagctgag gccaaggcat
     2401 gggaggagcg gttcttgttc gcggccttta tagcgagagt tcgtgataac ggtaagaaat
     2461 tgcagggcca gcgcttggat tttgtcgtct gaaaggaaat cgaaatacaa aacaagagaa
     2521 accgggcgaa gacccggttt caatcgtcat gggcggagcc tctgtcagct ataacaatct
     2581 ctcaaaaaca tcgatccttt ccacattgga gccgcccgcc gccgaagtcc tagcgccttc
     2641 gcggagggta gagatgtttt ggcagtcgga aaatgtcgct cccgtggaaa gtccaacccg
     2701 tttcgcgcac cccggatttc aattcgtgtc gcgaacggcg ccccattaga cctttgagag
     2761 ctgatttaag catcggtcct ccgccttctt ctgccgcggc cttgtgtgtt cgaggagaaa
     2821 gccacggaat tcgacgtaaa gcctcctcac gcccatgttt tccgcgggcc gcgggaactt
     2881 tacggaaggg acagtgcgcc gaatacggct agccgtcgtc gaagcctcgt ccctgggaaa
     2941 agcccagcgg tttgcgcggc acaccagcgg cattcaccgc tggacacgca acaaaactag
     3001 cgatgacaga cggggcaact tacggcccga ttccatctta tcgcgattct gatggtgtgg
     3061 ggggaggctt aacaaaaggt aaatataggc tcggagggtg agcggccgcc gagtttgcct
     3121 agtctattgg tttggttagg ttttgtgtcg caatgtagag agcagcaaat tgaagagaaa
     3181 cagtaaattt atttttaaga aaaagtgaaa aaa
//
LOCUS       DATMGX010001064         3371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_347149, whole genome shotgun sequence.
ACCESSION   DATMGX010001064 DATMGX010000000
VERSION     DATMGX010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347149"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VNR65_17475"
     CDS             complement(<1..456)
                     /locus_tag="VNR65_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWJ20511.1"
                     /translation="MNRHFTTAEAILAVPLDEPERLFAYDRSTLKQEWHRLAALWHPD
                     RNHLEPRAQEVLQHINVLYEIALQKIALGQWEGHGVLALPEDGSGSQRDLPYLRMRRF
                     ELGECYVGETFVAYALEKANRDLFDAAIRTIDRFRHADDAMKREVSHALP"
     gene            557..1219
                     /locus_tag="VNR65_17480"
     CDS             557..1219
                     /locus_tag="VNR65_17480"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20512.1"
                     /translation="MHYEPRPILTVDITLFTVLGGELRVALQHRDKPPFEGELALIGG
                     YVHVDEDASTLATAARVLKDKAGLEGIFLEQLMTFSGPERDPRGWSASVAYYALVPAE
                     KLSQAVPRDLVLISVDSIAGLPFDHETIIGTAVKRLRTKGAYSSLPAYLLPPAFTLPQ
                     LRDIYEKVMGVNLNDSAFRRKIEELQLIEPVPGEFSKASARPAKLYRLRTTALREFDR
                     KL"
     gene            complement(1234..2277)
                     /locus_tag="VNR65_17485"
     CDS             complement(1234..2277)
                     /locus_tag="VNR65_17485"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWJ20513.1"
                     /translation="MTDTLRNQTNRDVQRSERRSLLAGLAQFQGPSRWRSIFQFSSTA
                     LAYVATVAAMYAALQYSVWLTLAMSIPAAGLVVRLFIIQHDCGHGSYFRSPRANELVG
                     LFCSVVTFTPYANWKRQHAAHHAVWNNLDERHGGSDIYSACLTLQEYLDLSPRRRWLF
                     RVSRHPLIANLILPPLVFLLLFRIPLDAPRTWRKERRGVYLTNLALAIMLVGLVALPG
                     WRAVLLVQLPIMIFASVAGVWLFSVQHRFDDSEWLRQAEWTPVRAALNGSSYLKLPRV
                     LQWFTGNIGFHHIHHLLPRIPNYRLEACHRTLVAQGGVARTLTLADAVRAPSFARYLC
                     RTTLTKTRLWTGR"
     gene            2713..>3371
                     /locus_tag="VNR65_17490"
     CDS             2713..>3371
                     /locus_tag="VNR65_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HWJ20514.1"
                     /translation="MPAPKVLMVFPRFNPNSFWSMQAACDAWGAKCPAPPLGMITLAA
                     LLPREWNIKLVNRNAEDLKPEDIDWADMVMTGGMLPQQKDTLLLIERCQARGKPVVIG
                     GPDPTSSPDVYGHADFLVLGEAEGIIQEFVDAWDAGERRGRYEAEKFQVDVTKSPIPR
                     FDLLNFKHYLHVGVQFSRGCPFNCEFCDIIELYGRVPRSKTNAQMLAELQTLYDMGYR
                     G"
BASE COUNT          725 a          992 c          983 g          671 t
ORIGIN      
        1 cggaagcgcg tgcgaaacct cgcgtttcat cgcatcgtcg gcgtgacgga acctgtcgat
       61 ggtgcggatg gcggcatcga acaggtcgcg gttcgccttc tccaaagcat aggcgacgaa
      121 tgtctcgccg acatagcatt cgccgagttc gaaccggcgc atacggagat agggcaagtc
      181 ccgctgcgat ccgctgccgt cttctggcag cgccagcacg ccgtgccctt cccattgtcc
      241 caacgcgatc ttttgcagcg cgatctcgta gagcacgttg atgtgctgca gaacctcctg
      301 cgcgcgcggc tccaggtggt tgcgatccgg atgccagagc gcggccagcc tgtgccattc
      361 ttgcttgagc gtgctccgat cataggcgaa caggcgttca ggttcgtcaa gcggcacagc
      421 cagaatggct tctgcggtgg tgaaatgcct attcatggcg cggaacctat cattgtaagt
      481 tgcaacatgc aagttgcact ttatggtgcg gcgtgcgatg acgttttgat cgccatttgg
      541 cattgctata cgcgctatgc attacgaacc tagacccatc ctcaccgtcg acatcacgct
      601 cttcaccgtg ctcggcggcg aacttcgcgt ggcgctgcaa caccgggaca agccgccctt
      661 tgaaggcgaa ctcgccctca tcggcggtta tgtccacgtt gatgaggacg ccagcacgct
      721 ggcaactgcc gcccgtgtgt tgaaggacaa agcggggctg gagggcattt ttctggagca
      781 gttgatgacc ttttcgggac cagagcgcga tccccgcggc tggtcggcgt cggtcgccta
      841 ttatgcgctg gtcccggctg agaagttgtc ccaagcggtg ccgcgcgacc ttgtgctcat
      901 ttcagtcgac agtatcgccg ggctgccttt cgaccacgaa acgattatcg gcactgcggt
      961 caagcgattg cgcaccaagg gcgcctattc ctcgctaccc gcctatctcc tgcccccggc
     1021 gttcacgctc ccgcaattgc gcgacattta cgagaaagtg atgggcgtaa acctcaacga
     1081 cagcgcgttc cgccgcaaga tcgaggaact gcaactgatc gagccagtgc ctggcgaatt
     1141 cagcaaggcg agcgcacgtc cggcgaaact ctaccggctc cgcaccaccg cgctgcgaga
     1201 gttcgacagg aaactctgat tagaaacaat atcttagcgc cctgtccaca ggcgggtttt
     1261 tgttagcgtt gtgcggcaaa gatatcgcgc gaagctcggt gcgcgtaccg cgtcagcaag
     1321 agtcagcgtt cgagcgacgc cgccttgggc gacgagcgtc cgatggcacg cttccaaacg
     1381 gtagttcggt atgcgcggca aaagatggtg aatgtgatga aaaccgatat tgcccgtgaa
     1441 ccactgcagg acgcgcggga gcttcaggta ggagctgccg tttaaagcgg ccctcacggg
     1501 tgtccactcg gcctgccgca gccattccga atcatcaaag cgatgctgca ccgagaacag
     1561 ccagacgcct gcgacagaag caaaaatcat gatcggcagt tgcaccagga ggaccgccct
     1621 ccagcccggc agcgctacaa gcccaaccag catgatcgcg agggccaagt tggtaagata
     1681 aacgccacgg cgctctttgc gccatgttcg cggcgcgtcg aggggaatgc gaaacagaag
     1741 caagaataca agtggcggca ggatgaggtt ggcgatcagc ggatgacggc taacgcgaaa
     1801 tagccatcgg cggcgcggtg ataaatccaa atactcttga agcgtcaggc aggctgagta
     1861 aatgtcagag cctccatggc gctcatcaag attgttccac actgcatggt gggcggcatg
     1921 ctggcgcttc caattggcgt agggcgtgaa cgtcacgacg ctgcaaaaca agcccacgag
     1981 ctcgttggcg cgcggggaac ggaagtagga gccgtgtccg caatcgtgct gaatgatgaa
     2041 aagccgcaca accagaccgg ccgcggggat ggacatcgcc agggtgagcc aaaccgagta
     2101 ttgcaaagcc gcatacattg ccgcgacagt cgccacgtag gcaagcgcag tcgatgagaa
     2161 ctggaagatg ctgcgccacc ttgatgggcc ttgaaactgc gcaaggcccg cgagcaaaga
     2221 ccggcgttct gagcgctgga cgtcgcgatt ggtttggttg cggagcgtat cagtcatcaa
     2281 ttgtctttca gccgcctgat cctggtcatc agtcccgatg ggccgcgaga acgctcgcaa
     2341 aaaatggacc taacgccgcc agatattgcg gaacggtagt tttggctctg gcgagcttaa
     2401 gcgtcttcaa tatgcagacg cgaattcgcg ggcgacaaca tcattcttca aaatcatgct
     2461 caccacgcca ccgctgttcg taaatgtggc gtcaccgccc cgcttccggt agagtcggaa
     2521 actacctaca tcccgctcta tctggcgagt tgcaactttt tttgccgggc gctgccacga
     2581 accttcaagt cgcgaattag cactatcgaa ccgagggcgt cctgcattgg ctgcgtaggg
     2641 tattcgacca caaaccctgc ttgcctaaat acagtgcctt cgattgatgg gttcaccttg
     2701 aagggacatg ctatgccggc gccgaaggtt ctgatggttt tcccgcgctt caacccaaac
     2761 tccttctgga gcatgcaggc cgcctgcgat gcctggggcg ccaaatgccc cgctccaccg
     2821 cttggtatga tcactctcgc ggcactcctg ccgcgcgagt ggaacatcaa gctcgtcaat
     2881 cggaacgccg aagacctcaa gccggaggac atcgattggg ccgacatggt catgacagga
     2941 gggatgctcc cccagcagaa agacacgctt ctgctcatcg aacgctgcca ggcaagaggc
     3001 aagcccgtgg ttattggcgg ccccgatccg acatcgagcc ctgacgtcta tggccacgct
     3061 gattttcttg ttttgggtga agccgaaggc atcatccagg agttcgtgga cgcctgggat
     3121 gcaggggaac gccgtggccg ctatgaggcc gaaaagtttc aagttgatgt aacaaagagc
     3181 ccaattccgc gtttcgatct cctcaatttc aagcactacc tgcacgtcgg cgttcagttc
     3241 tcgcgcgggt gcccattcaa ctgcgaattc tgtgacatca tcgagcttta tggccgggtc
     3301 ccgcgctcga agaccaacgc gcagatgctc gccgaactgc aaaccctcta cgatatgggc
     3361 tatcgcggcc a
//
LOCUS       DATMGX010001065         3277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_439218, whole genome shotgun sequence.
ACCESSION   DATMGX010001065 DATMGX010000000
VERSION     DATMGX010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439218"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..2783)
                     /gene="mgtA"
                     /locus_tag="VNR65_17495"
     CDS             complement(153..2783)
                     /gene="mgtA"
                     /locus_tag="VNR65_17495"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF011702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HWJ20515.1"
                     /translation="MNVFSKPMQETDRIGVEPAAHDGLLAAVSRLQPREACAKLGSAL
                     EGLSDTEAGARLKKFGPNIVARERKATILEELWGRARNPLNALLLTLATTSYFLGDVR
                     AAVVIAVMVILAITTAFVQEHRSNEAAAKLRAMVHTTASVRRVPSRSEDPFSEIPMER
                     LVSGDVVRLSAGDMIPGDLRLLEAKDLFINQSALTGESMPAERHAPACDGGSEDALAL
                     PNLCFMGANVVSGYATGVIVQTGSKTFFGQLAHEIAGRRVPTAFDQGINKFTWLMIRF
                     ILVMVPAVFLINGVTKHDWLEALLFAVAVAVGLTPEMLPMIVTVNLAKGAMAMSRKKV
                     IVKRLNAIQNFGAMDVLCTDKTGTLTQDRIILKRHLDIQGRDSERVLQYAFLNSHFQS
                     GLRNLLDKAVLAHVELEQTLNIGSGYSKLDEIPFDFSRRRLSVVVAREDGKHILICKG
                     AVEEIFSVCTNYALDAEVGRLDETHFEAAKQQTIALNSDGFRVVAVAYKEMEASKTTY
                     SIEDEGGLTLLGYIAFLDPPKESAREAIAALARKGVQVKVLTGDNEIITRKICHEVQL
                     DPGEILLGARIAQMSEAELAEIADRTTVFAKLNPAQKEQVVRALHAKGHVVGFLGDGI
                     NDSPALKAADVGISVDTAVDIAKESADIILLEKSLLVLAEGVTEGRKVFANITKYIKM
                     GASSNFGNMFSVLGASIFLPFLPMAPIQVLTNNLLYDFSQTTIPTDNVDEEYLASPRK
                     WDIGNIFKFMVFIGPISSIFDYATYGMMLYVFDAWNNPSLFQTGWFVESLLTQTLIIH
                     IIRTAKVPFIESHASPALIATTVIICTVGMTLPFTWAGSALGFEPLPWHYWPLVAAML
                     VTYAVLTHLVKVWFIRRWGL"
     gene            3005..>3277
                     /locus_tag="VNR65_17500"
     CDS             3005..>3277
                     /locus_tag="VNR65_17500"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HWJ20516.1"
                     /translation="MTHTIRQKKKLLARVGRIRGQLEAIERAMSEEAECERIMHMIAG
                     IRGGVAGLMAEVVEDHIRTHLVDPERNPGALNADAADQLIEVVHTYL"
BASE COUNT          705 a          959 c          930 g          683 t
ORIGIN      
        1 ccggtcgcgc gggcgagccc ccggaccaaa ggacggaaag gatcagcact attgtcaatc
       61 gaaaggccaa ccgcctcggt gtggcggcgg attgatgacc gctggcaagc attgacccct
      121 cgaagcatta tccggatcac gcagcacact cctcaaagcc cccacctgcg aatgaaccat
      181 actttcacaa ggtgggtgag gacagcgtat gtcaccagca tggccgcgac cagtggccag
      241 tagtgccagg gaagcggctc gaaacccaat gccgaacccg cccaggtgaa gggcaacgtc
      301 attccgaccg tgcagatgat caccgtcgtc gcaatgagcg cggggcttgc atggctctcg
      361 atgaacggga ccttcgccgt tctgataatg tgaatgatga gggtctgggt cagcagagac
      421 tcgacaaacc atccggtctg gaacaacgac ggattgttcc aggcatcgaa cacgtagagc
      481 atcatgccgt atgtcgcata gtcgaatatc gagctgatcg gtccgataaa caccatgaac
      541 ttgaatatat tgccgatgtc ccattttcgc ggtgacgcca ggtactcctc gtcgacatta
      601 tccgttggaa tggttgtctg cgaaaaatcg tagagaagat tgttggtcag gacctggatc
      661 ggcgccatcg gcaggaacgg caggaagatg ctcgccccaa gcacgctgaa catgttcccg
      721 aaattcgagc tcgcacccat cttgatgtat tttgtgatat tggcaaaaac cttgcggccc
      781 tccgtcacgc cctcggcaag aacgagaagg ctcttttcga gcaggatgat gtcggccgat
      841 tccttggcga tgtcgaccgc cgtatccacc gagatcccga cgtccgcggc tttcagggcc
      901 ggactgtcgt tgatcccgtc gccgagaaat cccaccacat gtcctttggc gtgaagcgcc
      961 cgcacgactt gttccttctg cgcagggttc agctttgcaa ataccgtcgt acggtcggca
     1021 atttccgcca gctcagcttc gctcatctga gcgatcctgg ccccgagcag gatttcgccc
     1081 gggtccaatt ggacctcatg acaaatcttg cgcgtgatga tttcgttgtc gccggtgagg
     1141 accttcacct gtacgccttt gcgcgccagc gccgcgattg cctcccgggc gctctctttc
     1201 ggcggatcga gaaaggcgat gtagccaagc agcgtcaagc cgccctcgtc ctcgatcgag
     1261 tatgtggtct ttgatgcctc catctcctta tacgcaaccg cgacaacacg aaatccgtcg
     1321 gagttaagcg cgatcgtctg ctgtttggcc gcctcaaaat gggtttcgtc aagccgtccg
     1381 acttccgcat caagggcgta gttcgtacac accgaaaaaa tctcttcgac ggcccccttg
     1441 caaatcagaa tgtgcttgcc gtcttcccgc gcgacgacga ccgatagacg gcggcgcgaa
     1501 aaatcgaagg ggatttcatc gagctttgaa tacccgctcc cgatgttcaa tgtctgctcg
     1561 agttcgacat gcgccaaaac cgccttgtcg agcaggttcc tgagccctga ctgaaagtgg
     1621 ctgttcagaa aggcatactg aagcaccctt tcggagtccc gcccctggat gtcgaggtgc
     1681 cgtttcagga tgattcggtc ttgcgtcagg gtgccggtct tgtccgtgca cagcacgtcc
     1741 atcgctccga agttctggat ggcgttgagg cgcttcacga tgaccttctt gcgcgacatt
     1801 gccatcgcac ccttggccaa gttcaccgtg acaatcatcg gtaacatctc tggcgtgaga
     1861 ccgactgcta ccgcaacggc gaacaataga gcctctagcc aatcatgttt tgttacgccg
     1921 ttgatgagga aaactgcggg gaccatgacc aagataaagc ggatcatcaa ccatgtgaat
     1981 ttgttgatac cctgatcgaa ggctgtcgga acgcgtcgtc ctgcaatctc gtgagcaagc
     2041 tgcccaaaaa acgttttcga gcctgtttgc acgattaccc cggtcgcata gccggatacg
     2101 acgttcgcgc ccatgaagca gaggtttggc agggcgagcg catcctctga accgccatcg
     2161 catgccggag catgcctttc cgcaggcatg gactcgcccg taagagcaga ctgattgatg
     2221 aaaagatcct tagcttccag taagcggagg tcgccgggta tcatatctcc tgctgaaagc
     2281 cgcacgacgt cgcccgacac cagccgctcc atcgggattt cggagaacgg atcctccgag
     2341 cgactgggga cgcgcctgac gcttgctgtg gtgtggacca tggcgcgtag cttggcagcg
     2401 gcctcatttg accgatgttc ctggacgaag gcggtcgtta tcgcgaggat taccattact
     2461 gcgatcacga cagcagcacg gacgtctccc aaaaaatagg aagtagtcgc cagcgtcagc
     2521 aacaatgcgt tcaatggatt acgggcacgg ccccaaagtt cttccaggat tgtcgccttg
     2581 cgttcccggg caacgatatt cggcccgaat ttcttcaggc gtgcgccagc ctcggtgtcc
     2641 gacaggcctt ccaatgcgga cccgagtttc gcgcaggctt cgcgaggctg aagtcggctt
     2701 actgcagcaa gcaacccatc atgggcagct ggttccacac caatgcgatc ggtctcctgc
     2761 attggcttcg agaagacatt catgaacggt atcgtgatgt ttcagtccgc ggcacgaggc
     2821 accaccgggt tgacggccgg tagtgaacat cattgcaaag tcgacgttgg gtgttgcggc
     2881 agatcaaagc ccagaatcct ctgccaggct gcgatgtagg gtgcctgcgg cggctgggtt
     2941 ctggcccgtt cattggcgtt gatcatcgtt gacatggcat aggggggtac cctatgaagc
     3001 gggcatgaca cacacgatcc ggcaaaagaa aaagctgctc gcgcgcgtgg ggcgcattcg
     3061 cggccagctc gaggcgatcg agcgggcgat gagcgaagag gccgaatgcg agcgcatcat
     3121 gcacatgatc gccggcattc gcggcggcgt cgccggcctg atggccgaag tggtcgagga
     3181 ccacatccgc acccatctgg tcgatcccga acgaaatccc ggcgcgctca atgccgacgc
     3241 cgccgatcag ctgatcgaag tggtccacac ctatctg
//
LOCUS       DATMGX010001066         4006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_213313, whole genome shotgun sequence.
ACCESSION   DATMGX010001066 DATMGX010000000
VERSION     DATMGX010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213313"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1278)
                     /locus_tag="VNR65_17505"
     CDS             complement(join(83..1029,1029..1278))
                     /locus_tag="VNR65_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611875.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HWJ20517.1"
                     /translation="MKQSRFTEERIIAILREQEAGSRTGDVCRKHGISSATFYKWKAK
                     YGGLDVSEARRLKVLSDENAKLKKLLAEAMLDNAMLKDLKFKKMVTPVARRQAVAHLC
                     SSFEVSQRRACEVIGADRSSVRYLSLRPDDATIRVRLRELASVRRRFGYRRLLLMIRS
                     EGVLINHKKLRRLYAEERLQVRRRGGRKRALGTRAPIALPQGPNQRWSLDFISDTLTD
                     SRRFRILAVVDDFTRECIALVADTSLSGTRVGRELDAVIARRGRPMMIVSDNGTELTS
                     MAILRWSQLTRIEWHYIAPGKPQQNAFVESFNGRLRDELLNETLFSSLDHARELLAEW
                     QDDYNTVRPHSGIGNLPPSTYAKLTASDMQRDGTLRCVEGSAPRPVASPSHTGSNDQR
                     ILPIAG"
     gene            complement(1477..2520)
                     /locus_tag="VNR65_17510"
     CDS             complement(1477..2520)
                     /locus_tag="VNR65_17510"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HWJ20518.1"
                     /translation="MTQQIHFAAIAERDSCADVIFVHGLTGDAVGTWTSHGASEPEGE
                     YWPKWLSSDLPNLSFYTLGYPASIFAQWAKKEMALYERAKNSLELLAGYDFGKRPIIF
                     ICHSLGGLLTKQILRTAKESADAGWQRIADNCVGILFLATPHTGSSLANLLKAFSGPF
                     SSTHIDKLLKDSSELEELNESFRAFCYRRQIDVFAYYEKYKTHGTAIIVDKASADPGV
                     NGIGPVPVDANHLDICKPSSRHSLIYLSVFRRLEALQTRLGHIATKGAFAADDLHEAN
                     VLDRRDLHAKMSAASRKNEYLFANDSQNRFARSFARQGLMTGASQLHNNLLADVEQRF
                     QSLIFHPLILRWC"
     gene            2797..3381
                     /locus_tag="VNR65_17515"
     CDS             2797..3381
                     /locus_tag="VNR65_17515"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWJ20519.1"
                     /translation="MSLGRQAKILSKGQTEAILGYLSNSRYPIRNRVIFLLSIKAGLR
                     AKEIASLTWEMITDASGEIGRAIHLQDKASKGRSGRIIPLNEELRSALGDWHRRVWTT
                     PSAFVISTERSKQTSAQNVVNLFARWYSELGFHGCSSHSGRRTFITNTARKISSVGGS
                     LRDIQILAGHTNLRTTQRYIEADLNAQKRVVQQI"
BASE COUNT          915 a         1108 c         1059 g          924 t
ORIGIN      
        1 tattgggcag cgggttggct gccttctgaa ccgcgcatga cgagacgaca tcccctacga
       61 ctgatggcct gagccccaaa gatcatccgg cgatgggcag aatcctttgg tcatttgagc
      121 ctgtgtggct cggtgatgca acgggacggg gcgcggagcc ctcgacacag cgcagcgtcc
      181 cgtcccgttg catgtcggac gctgttagtt tggcataggt tgatggcggc aagttaccga
      241 tcccgctgtg cgggcgaacg gtgttgtagt catcctgcca ttcggcaagc agttcgcgag
      301 cgtgatcgag tgacgagaac aacgtctcgt tcaacaactc gtcgcgcagt cgtccattga
      361 agctttccac gaaggcattc tgctgcggct tgcccggcgc gatgtagtgc cactcgatcc
      421 gggtgagctg cgaccagcgc aggatcgcca tgctggtgag ctcggttccg ttgtcgctga
      481 cgatcatcat tgggcggcct cgtcgagcga tcacggcatc gagctcacga cccacccggg
      541 tccccgacaa cgacgtgtcg gcgaccagtg cgatgcattc ccgggtgaag tcatccacga
      601 cagccaggat acggaagcgt cggctgtcgg tcagcgtgtc gctgatgaaa tccagtgacc
      661 agcgctggtt cggtccctgc ggcaacgcga tcggagcccg tgttccaaga gctcttttac
      721 gtccacctcg ccgacgaacc tgcagccgct cctcggcata tagccgcctc agcttcttgt
      781 ggttgatcag cacgccttcg ctgcggatca tcagaagcag acggcgatag ccaaagcgcc
      841 ggcgtaccga cgccaactca cgcagccgga cgcgaatggt cgcgtcgtcg ggccgaaggc
      901 tgagataacg caccgaggac cgatcagccc caatcacttc acacgcccgc cgctgactga
      961 cctcaaaact cgaacaaagg tgagccacag cctgccgcct tgcgacgggc gtcaccattt
     1021 ttttgaattg aggtccttca gcatggcgtt gtcgagcatc gcttcggcca acagcttctt
     1081 cagcttcgca ttctcgtcgg aaaggacctt gaggcgccga gcttctgata cgtccaggcc
     1141 accatacttg gccttccatt tgtagaacgt cgcgctgctg atcccatgct tgcggcaaac
     1201 atcgcccgtc ctcgaacccg cttcctgctc ccgtaggatc gctatgatcc gctcttccgt
     1261 gaatctcgac tgcttcatct ccgtctccct caaggtgacg gatcctaccc atctttggag
     1321 gaagttcagg ggctcaggtc acatcgaatg tggcatcgtt cggtcagaaa gtagagtgcg
     1381 ttcattatag ttttggcact tgcttcgttc gctgtatatt tatgactcaa aggatcaatc
     1441 acctgagctt gcacggctgc ggacacagtc gcgttgtcag caccatcgca aaattaaagg
     1501 atggaaaatc agagattgaa atcgctgctc gacgtctgcg agcaggttgt tgtgaagctg
     1561 gcttgcgcct gtcatgagtc cttggcgtgc aaagcttcgc gcaaacctgt tctgtgaatc
     1621 attcgcaaac aagtactcat ttttccggga agcggccgac atctttgcat gcaaatctcg
     1681 tcgatctaag acgttcgctt catgtagatc atctgctgcg aacgctcctt tggtcgcgat
     1741 gtgaccgagt cttgtctgga gtgcttccag cctacgaaaa acactgaggt aaataaggct
     1801 atgccgacta gatggtttac atatgtcaag atgattggcg tcaaccggca ccggcccaat
     1861 accatttacc cccgggtctg cgcttgcctt gtcaacgatg attgccgttc catgcgtttt
     1921 atacttctca tagtatgcga acacatcgat ctgccgacga tagcaaaaag ctcgaaagct
     1981 ttcgttgagc tcttcaagct ctgagctgtc cttgagcagt ttatcgatgt gggtagagga
     2041 aaacggtccc gagaatgcct taagaaggtt agctagactc gatccggtat gtggagttgc
     2101 aaggaataga atgcccacac aattgtcggc aatcctctgc catccagcat ctgccgattc
     2161 tttggcggtc cgcagaatct gcttcgtaag cagtccacca agactgtgac agatgaagat
     2221 tataggcctc ttgccgaaat cgtatcccgc caacagctca agagaattct tggcccgttc
     2281 ataaagggcc atctccttct tcgcccattg agcaaaaata ctggccggat agccgagggt
     2341 gtagaagctt aagttcggaa gatcgcttga gagccatttt ggccaatatt caccttcagg
     2401 ctccgaggct ccgtgcgaag tccacgtccc gaccgcatcg ccagttaatc catgaacaaa
     2461 aatgacatcg gcgcagctgt cgcgctctgc aatggctgca aaatgtatct gctgtgtcaa
     2521 ctgaaaatcc cgaacgtttc tttttatgca accctagatg taatcgtttg agattgaaag
     2581 cttcttttga aaccgcccag tcgccgctgg gagtattccc gcttaacgtc gagactttag
     2641 tagcagaaag actggtgata cttggaccct tttataacca gtcctagcaa gccattgacg
     2701 cgataacgaa tattttcagc cgaccctgtt cgatcacccg tgactggtta caatccagca
     2761 tcctgatgac ttttataaat cagtcaggag aaccgaatgt cgctcggaag gcaggccaaa
     2821 attctaagca aaggacagac cgaagccatc ctcggttatc tgtcaaactc ccgctacccg
     2881 attcgaaacc gagtgatttt cctcctgtcg atcaaggccg gccttagggc taaagaaata
     2941 gcctctctga cctgggagat gatcaccgat gcatccggcg agataggtcg agccatccac
     3001 cttcaggaca aagcgagtaa aggccggtcc ggtcggatca ttccactgaa cgaggagctc
     3061 cggtcagcgt tgggcgattg gcaccggagg gtctggacca cgccatccgc ctttgtgatt
     3121 tcgacggaac gatcgaaaca aacatcagcc cagaacgtcg tgaacttatt cgcgcgatgg
     3181 tacagcgagc tcggattcca cggttgctct agtcatagcg gacggcgtac gttcataacg
     3241 aacacagccc gcaagatatc gagcgtgggt ggatcgctcc gcgatattca gatattggct
     3301 ggccacacca atcttcggac gacacagcga tacattgagg ctgacctcaa tgcgcagaag
     3361 cgcgtggtgc agcaaatcta ggagactcat ttcccacgat ctcctgagat taccaaaaat
     3421 taagttgtac gaaatgcgtg cttcaaacat atcttgaggg aacgagattg ctacgacaca
     3481 caaggaggtc cgcaaaccga aggggggtga tacaagtgac cctagaaacc atcgtgcacc
     3541 tgacgatagc aagcctctac atagtattgg ctttctgcca ctgagctctg ggagccaggc
     3601 agttgctgct taaagaccaa ttcggtgctg caacgcacca agaagccccg gccagaagct
     3661 ggggcttctt ttttgttgct cagtcacagc tgagtttgac attggatgtc gttcaagcgt
     3721 cgacacatcc taaatatctc gaaatctcag tatcgacgcg accctatcag cgtcccgtaa
     3781 cctcctaggt gtgaggcact gcggaaaccc cggtggtctg agacaccggt cggagcattg
     3841 gtatgcggtg tttcgaggtc accttcctga tgatgtcctg atctgcgagt ctgatggttc
     3901 cggtaaccgt taggcttaga atggtcacag tggataatgc tataagccgg ttcgtcgact
     3961 tgaggtgttc accggcgcag gtcgccggcg cacgtggagc gccgag
//
LOCUS       DATMGX010001067         2407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_243585, whole genome shotgun sequence.
ACCESSION   DATMGX010001067 DATMGX010000000
VERSION     DATMGX010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243585"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..943)
                     /locus_tag="VNR65_17520"
     CDS             complement(206..943)
                     /locus_tag="VNR65_17520"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ20520.1"
                     /translation="MVAAANLVEVAALVGDTARATMLNALMGGQSLTATELAYCANVS
                     RSTASGHLSKLVAARLLTVIRERRFSYYRIASPLVATMLESIKVVAAIEVPPRRQPRS
                     ANDDALRFARSCYDHLAGQVGVAVTDALVAMGHIVLTDEGGEVTSSGERFLSGFGVDL
                     RPRTRRIFCQPCLDWSERRYHLKGLVGARILDRLLGLGWLKCVSGSRALQLTSSGRAG
                     LSETFQIEINNEGVPTGRICDPRRLTA"
     gene            1140..2141
                     /locus_tag="VNR65_17525"
     CDS             1140..2141
                     /locus_tag="VNR65_17525"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HWJ20521.1"
                     /translation="MGMAAVIYKKGAPDNFVWEEIKVGSPERGQVRLRSTAVGVNFAD
                     TYHRAGIPHPMIVGDPPVVVGFEGVGEIEELGPGVTGFSVGERVCTCLPPLGAYSQER
                     LYPADKLIKVPKDLPLDDVQLAGLILKGMTAQYLLHRTHKVQPGDYVLIHAAAGGMGH
                     ILCPWARHLGATVIGTVSTDAKAEIARNLGCHHVINYSTEDFVAVTRKITDGKGVDVV
                     YESIGKDTLQRSLDCLRLVGMCAAYGQASGVPDPINLVEDLGVRGSLMITRPVLSHYM
                     SNRSEIDAGTKSLFDAVAQGVVASNVVKTFPLREAAAAHKFIEGRKTTGSIVMLPFE"
BASE COUNT          530 a          695 c          711 g          471 t
ORIGIN      
        1 ttagcagtgg ccggcgattg tccgctgtta ctccaatagc gaccaggaat gttgcaacgc
       61 cgcgaatgtc gcgatgtgcc aatcgcttca cttcgcactg caagaaaagc agcgaccttt
      121 ctcggcgtca gaccaccatg aactcggcga tagacctgcc aaacggtgac ccgcagcatt
      181 tgcgtgagat gacgcttcgc tcagcctaag ctgtcagccg tcgtgggtcg catattcgac
      241 cggttggcac accctcgttg ttgatctcga tctggaaggt ttcggacaag cccgcccttc
      301 cagacgacgt tagttggagt gcacggctgc ccgacacaca tttgagccat ccgagcccca
      361 atagacgatc cagtatccga gctccgacga gtcccttgag gtgataacga cgctcgctcc
      421 agtcgagaca aggctggcag aaaatccgcc gagtccgcgg ccgcagatca acgccgaacc
      481 cggaaaggaa acgctcgccc gaagacgtga cctcaccgcc ttcatcggtg agtacgatgt
      541 gccccatggc cacaagtgca tcggtgacag cgactcctac ctggccagcc aggtgatcgt
      601 agcaactccg ggcaaaacgg agtgcatcgt cgtttgctga ccgcggctga cggcggggtg
      661 gcacctctat ggcggcgacg accttgatgc tctccagcat tgtcgcgacc agtggggacg
      721 cgatccgata atagctgaag cggcgctcgc gaatgacagt cagaagccgc gcggcaacca
      781 gcttgctcag gtggccgctc gccgtcgagc gagagacatt ggcgcagtaa gcaagctcag
      841 tggccgtcag cgactggccg cccataaggg cgttaagcat cgtggctcgc gcagtgtcac
      901 ccacgagagc ggccacttcc accagatttg ccgccgcgac catccttaac cctatcgctg
      961 acaccggaag catatcaatc gaaaacagtt tggcatgggc cgaactgttc gcgcgagaca
     1021 attgcgaacc tcgcggtaag aatgcgctgc agccctagtc tagcaagggc gctcacggtt
     1081 ggcatggagg atgagatggt cgccgttgcg acgatgttta ataacaggag gaacgcatta
     1141 tgggaatggc ggcggtaatc tacaaaaaag gcgcacccga caacttcgtg tgggaggaga
     1201 tcaaggtcgg gtctcccgag cgtggccagg tacgtcttcg cagcacggcc gtcggcgtga
     1261 atttcgctga tacgtaccat cgagcaggta tcccgcaccc gatgatcgtc ggcgatccgc
     1321 cggttgtcgt cggtttcgaa ggggttggcg agatcgagga gttgggtccc ggcgtcaccg
     1381 ggttctcggt cggcgaacgc gtttgcacct gccttccccc gctcggcgcc tatagtcagg
     1441 agcgccttta cccggctgac aagttgatca aggttccaaa ggacttgccg ttggatgacg
     1501 ttcaactggc aggactaatc ttgaagggca tgaccgcgca atatctcctg catcggacgc
     1561 acaaggtgca gccgggagat tatgttctga tccatgcggc tgctggcggc atgggccata
     1621 tcctttgtcc ttgggcgcgg catctcggag ccactgtgat cggtaccgtt agtaccgacg
     1681 cgaaggccga gatcgctcga aatctcggtt gtcatcacgt tatcaattac tcgaccgaag
     1741 acttcgtagc tgtcacccgt aagattacgg acggaaaagg cgtagacgtt gtctatgagt
     1801 cgatcggtaa ggacacgctg caaaggtcac tcgactgcct gcggctggtc ggtatgtgcg
     1861 cagcttacgg ccaggcgtca ggcgttcctg atccgatcaa cctcgtcgaa gatttgggcg
     1921 ttcgcgggtc tctcatgatc acccgtcccg tgctttcgca ttacatgtcg aaccgaagcg
     1981 aaatcgacgc gggaacaaaa tccctgttcg atgccgtcgc acagggggtg gtagcgagca
     2041 acgttgtcaa aacctttccg cttcgggagg ctgcagccgc gcacaagttc atcgagggac
     2101 gcaagacaac gggctcgatc gtcatgcttc cattcgagta agcggccgca ataagggcgg
     2161 cccaacgggg ttactggttc atcgcccaac atgcctgaac agcacaagcg cttttgcgcc
     2221 tacgtgaaag gacggttccg atgtcgatta agcactatag ggttcgaaca atcacagacg
     2281 aaaaccgagg tcagccgatt atcagtcaga cggtggaaca cggcaacgtc gtctacttgg
     2341 ccggcataac gccggacccg atcgagggct gcatcaaagc gcagaccaga caagtactgc
     2401 agcgcgt
//
LOCUS       DATMGX010001068         2636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_142772, whole genome shotgun sequence.
ACCESSION   DATMGX010001068 DATMGX010000000
VERSION     DATMGX010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142772"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VNR65_17530"
     CDS             complement(<1..415)
                     /locus_tag="VNR65_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20522.1"
                     /translation="MNESNNLSQFSVPSTRRGPPRFVRNLAQRMLAGELTLILPKGRR
                     LLLSGEKNGPEVTVEIHRFRALWRAITRGELGLAESYMNGEWKASDLVGLMELALTAK
                     AGFEWLVKIYSSIDFINRHRSLRRPNTRAGAAANIA"
     gene            complement(408..1211)
                     /locus_tag="VNR65_17535"
     CDS             complement(408..1211)
                     /locus_tag="VNR65_17535"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HWJ20523.1"
                     /translation="MQHSAIYAGAVAHKRFRPRPHRLRYRVFSLLIDIDELPQLGRRL
                     RLFAHNGFGLFSFHDRDHGDGGGGLRLWAERHMAAAGVKPDGGAIRILCYPRIFGYVF
                     NPLTVYFCHARSRELVAIFYEVSNTHRERHTYVIPVTGPATPLLRQTCRKEFYVSPFI
                     PMNCTYHFRVASPQDSLSILIEEVDDEGVLLAAAFSGRRRPLSDAMLLRAFLAYPLMT
                     LKVVAGIHWEALRLIMKRTPVFPHKRANERIAVSIVPPPELERLRQADE"
     gene            complement(1211..2572)
                     /locus_tag="VNR65_17540"
     CDS             complement(1211..2572)
                     /locus_tag="VNR65_17540"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWJ20524.1"
                     /translation="MLSRVSFDPRSPGGEIAVVGSGIAGLSAAWLLSQRHPVTVFEKN
                     AQPGGHSNTVDAPAPRSVLPVDTGFMVYNEVNYPNLVALFRHLGVATKPADMSFAASL
                     DGGGFEYSSRDFDGIPRPSDNFLRPRFWSMIRGILRFYREVPALLDHPAIEGVTLGVY
                     LHHRSYSRAFVDDHILPMSAAIWSAAPGEIRNYPLNAFIRFFVSHRLTQLRNRLEWRT
                     IDGGSREYVRRMTEAFRPGLRTGLGVRKIVRESGSVIIEDETGRKRSFDHIVMATHAD
                     QALALLADADPEEKRLLGAFKYASNRTLLHTDPALMPKRRQVWSSWNFIAGEGSCADS
                     PPCVTYWMNRLQGIDSNSPLFVTLNPIHEPGSGHLLRDLTYEHPQFDAAALLAQRDLW
                     KLQGVRNTWFCGSYFGYGFHEDALQAGLAVAEELGGVRRPWSIANDLGRIVRASRQRV
                     DVA"
BASE COUNT          573 a          740 c          810 g          513 t
ORIGIN      
        1 acgcgatgtt ggccgccgca ccggcccgcg tgttggggcg tcgcagcgaa cgatggcgat
       61 tgatgaaatc gatcgacgaa tagattttga caagccattc gaaacccgct ttggccgtga
      121 gtgcaagctc cataaggccg acgaggtcgg acgccttcca ttccccattc atataggact
      181 cggcaagccc gagctcaccg cgagtgatcg cgcgccataa tgcacggaag cgatggatct
      241 cgaccgtcac ttctggaccg ttcttctcgc cgctgagcag aagccgccgt cctttgggga
      301 gaatgagggt gagctctcca gcaagcattc tctgggcgag gtttcggacg aaccgggggg
      361 gacctcggcg cgtcgaggga accgagaatt ggctgagatt attggattca ttcatcggct
      421 tggcggagcc tctcgagttc gggtggcggg acgatgctaa cggcgatgcg ctcattggcc
      481 ctcttgtggg gaaacaccgg cgttcgcttc atgatgaggc gcagcgcctc ccagtggatg
      541 ccggcaacga ccttgagcgt catcaaagga taggccaaga atgcgcgcaa cagcatcgcg
      601 tcgctaagcg gccggcggcg gccggaaaac gcggcggcga gcagaacacc ctcgtcgtca
      661 acctcttcga tcaggatcga aaggctgtcc tgcggcgacg caactcggaa atggtaggtg
      721 caattcattg gaatgaaagg cgaaacatag aactctttgc ggcaggtttg gcgaaggagc
      781 ggcgtggccg gacccgtgac aggaatgacg tatgtatgtc gttcgcgatg ggtgttgctg
      841 acctcgtaaa aaatcgcgac gagctcgcgg ctccgtgcat ggcagaagta gactgtcagc
      901 ggattgaata catagccaaa gattcgtgga tagcagagga tgcggatggc gccgccgtcc
      961 ggcttgactc ctgcggccgc catatggcgc tcagcccaca atctgaggcc gccgccgccg
     1021 tcaccgtgat cgcggtcgtg aaagctaaac aggccgaagc cattgtgtgc gaacaggcgc
     1081 agacgacgcc caagctgcgg cagttcgtca atgtcgatca agagcgaaaa aacgcgatat
     1141 ctcaagcgat gcggtcgagg ccgaaacctt ttatgcgcga ccgcgccagc gtaaatggcg
     1201 gaatgttgca tcacgcgacg tcaacccttt ggcgcgatgc gcgaacgatc cggccaaggt
     1261 cgttcgcgat ggaccatggc cgtctgacgc ccccaagttc ctcggccacg gctagaccgg
     1321 cttgaagcgc gtcctcgtga aatccatagc cgaaatagct gccgcaaaac catgtgtttc
     1381 gcacgccttg taatttccaa aggtcgcgct gagccagcag ggccgcggcg tcaaactggg
     1441 ggtgttcgta ggtcaaatct cgcagcaaat gccccgagcc aggctcgtgg atcggattga
     1501 gtgtcacgaa cagcgggctg ttggaatcaa tgccttgcag cctgttcatc caataggtca
     1561 cgcaaggcgg gctgtccgcg cacgatcctt ctcccgcgat gaaattccag ctcgaccaaa
     1621 cctgtcggcg cttcggcatc agtgccggat cggtgtgaag cagcgtgcgg ttcgacgcgt
     1681 atttgaacgc gcccaggagg cgtttttctt cagggtccgc gtcagcgagc agagccagcg
     1741 cctggtcggc gtgcgtcgcc atgacgatat ggtcgaacga cctcttgcgt cccgtctcat
     1801 cctcgattat gaccgaaccg ctttccctga cgatcttgcg aacgccaaga cctgtgcgaa
     1861 gtcccggcct gaatgcttcc gtcatacggc gaacgtattc gcggctgcct ccatcgatcg
     1921 tccgccattc cagccgattt ctgagctgcg tgaggcggtg gctgacaaag aagcgaataa
     1981 atgcattcag tggatagttg cggatttcac ccggggcggc cgaccagatg gctgcactca
     2041 tcggcagaat atgatcatcg acgaaggcac gggaatagct tctgtggtgg agatagaccc
     2101 caagagtcac tccttcaatc gcggggtggt cgagcagcgc tggaacctcc cggtagaaac
     2161 gcaagatccc gcggatcatc gaccagaagc gcggacggag aaaattatcc gatggcctgg
     2221 gaattccgtc aaagtctcgc gagctatatt cgaatccacc gccatcgagc gacgcggcga
     2281 aggacatgtc ggcaggtttt gtcgcaacgc cgagatgacg aaacagtgcg accaagttcg
     2341 gatagttgac ctcgttatag accatgaaac cggtatccac gggcaggacg ctgcgtggag
     2401 caggcgcgtc gaccgtattg gaatgcccgc caggctgagc gtttttttca aaaactgtta
     2461 cggggtggcg ttggcttagc agccaggcgg ctgacaatcc tgcaattccg ctccccacca
     2521 cggcaatctc gccgccgggc gaccttgggt cgaaggatac ccttgatagc attgaagaaa
     2581 ttcccaggtt tgcgcgtatt gcggggttcg catagagccg aagtatttac ggaggg
//
LOCUS       DATMGX010001069         4096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_434781, whole genome shotgun sequence.
ACCESSION   DATMGX010001069 DATMGX010000000
VERSION     DATMGX010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434781"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /gene="lhgO"
                     /locus_tag="VNR65_17545"
     CDS             <1..887
                     /gene="lhgO"
                     /locus_tag="VNR65_17545"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HWJ20525.1"
                     /translation="GLRMLNREQFREIEPHCDGIRGLQVPTTGIVDYTAVAQKYAELI
                     QRAGGEIVFRAKVLGLREEGDCNIVETSAGTFRARYVINCAGLYSDTITRMAGVHTDL
                     EIIPFRGEYYEVKPERRNLVKALIYPVPDPRFPFLGVHFTRRVNGSVEAGPNALLAFR
                     REGYTGTTPDLNEAMEMLLFPGFRKMARKYWRMGLAEQFRSWIKAAFVKSLQKMVPEL
                     QESDLAPGGSGVRAQAVDSNGNLVDDFHFVHSKGMIHVCNVPSPAATASLEIGKEIVD
                     MLAKRFELSPSSVQPVTS"
     gene            906..1856
                     /locus_tag="VNR65_17550"
     CDS             906..1856
                     /locus_tag="VNR65_17550"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWJ20526.1"
                     /translation="MAHYLVTGAAGFIGSALVRALLERGERVRGFDNFETGKREHIRE
                     VAGQIDFREADLLDLEALRSACQGLDYVLHQAAIPSVPRSVADPARSNAVNVDGTLNL
                     LIAARDAGVKRVVYAASSSAYGDTPTLPKHEGMPPNPISPYAVSKLAGELYMTSFHRV
                     YGLPTVSLRYFNIFGPRQDANSQYSAVLAKFITSMLQGEKSTIYGDGEQSRDFTYIDN
                     VVAANLLAATQPESQVAGQTFNIATGHRFTLNETFAILKELTGYSGEVEYAPARAGDV
                     KHSLADVSRAEAQLGYRTLVDFKEGLRRTVAWYRSVAVTA"
     gene            1916..3280
                     /locus_tag="VNR65_17555"
     CDS             1916..3280
                     /locus_tag="VNR65_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWJ20527.1"
                     /translation="MSLPSKATSSELSELLKRKIESRTAKVGVIGMGYVGLPLALLFS
                     EQKFPVTGFDIDKKKIDVLESGGSYIYRIAPTEIQAARTQGFTATSDYDNITHMDAII
                     ICVPTPLNEYHEPDLSYITATAESIGPHLRAGQLVVLESTTYPGTTEEVLVPILERSN
                     KRGLLAARAGTENDNRFYVVFSPEREDPGNDTVARCDIPKVIGGLDPQAGELGAKLYG
                     AIFNRTVPVSTPAAAEMTKLLENIYRCVNIAMVNELKMLCLRMGIDIWEVIDAAKSKP
                     FGFQAFYPGPGLGGHCIPIDPFYLSWKAKQFDFRTRFIELAGEVNTNMPYFSVEAVAS
                     ALNKEKKALNGSKILVLGLSYKKDIDDLRESPSLTIIELLQKRGAMVSYNDPYFPTVG
                     HGRRYALNMTCAPLENLDQYDCVVIVTDHSDYDYRRIVRESKLVVDTRNATKGIASPK
                     IVRC"
     gene            complement(3498..>4096)
                     /locus_tag="VNR65_17560"
     CDS             complement(3498..>4096)
                     /locus_tag="VNR65_17560"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWJ20528.1"
                     /translation="SNASAASRLKHLRSIAKLTALLLVLGPVGGSTVAIQPYPSAGGA
                     PMALVLKSPAFAHGAEIPKKHSCEAADVSPALEWGGPPARTVSFALIMDDPDAPAGTW
                     VHWVLWNLPASAHELSEAVAKQDQLDDGTRQGRNSFRKIGYNGPCPPPGKPHRYFFRL
                     YALEEKLDLAPGANNLDLQEAIKGHVLIQAEYMGTYRR"
BASE COUNT          862 a         1204 c         1208 g          822 t
ORIGIN      
        1 cgggtttgcg aatgctgaat cgcgagcagt ttcgcgaaat cgaacctcat tgcgacggta
       61 tccgcggtct gcaagtgccc accaccggca tcgtggatta cacggcggtg gcgcagaaat
      121 acgccgagtt gatccagaga gctggaggag agattgtgtt tcgcgccaag gtgcttggcc
      181 tgcgtgaaga gggagactgc aacatcgtgg aaacgagcgc cggcaccttc cgggcacgct
      241 acgtgatcaa ttgcgccggc ctgtatagcg atacgatcac tcgcatggcc ggggtgcaca
      301 ccgacctcga aatcatcccc ttccgcggag aatactacga agtgaaaccc gagcggcgca
      361 acctggtaaa ggcgctcata taccctgttc ccgatccgcg tttcccgttc ctcggagtgc
      421 atttcacgcg ccgggtcaat ggcagcgtcg aggcggggcc caacgctttg ctggccttcc
      481 gccgcgaggg atacacgggc acgacgcccg atctcaatga ggccatggag atgctgttgt
      541 tccccggctt tcgcaagatg gcgcgcaagt attggcgcat gggactggcc gaacaattcc
      601 gctcgtggat caaggcagct tttgtgaagt cgctacagaa gatggtgccg gagctacagg
      661 aatccgattt ggcaccagga ggttctggag ttcgggcgca ggccgtggac agcaacggca
      721 acctggtgga cgattttcat ttcgtccaca gcaaggggat gatccatgtc tgcaacgtac
      781 cgtcaccggc ggccacggca tcgctggaga tcggcaaaga gattgtcgat atgctggcca
      841 agcgattcga gctgtctcca agcagcgtcc agccagtcac ctcttagcat gctaaccttg
      901 agactatggc acattatctg gtgaccggcg cggccggatt catcggctcg gccctggttc
      961 gcgcgttgct ggaacgcggc gaacgagtcc gtggcttcga caatttcgag accggaaaac
     1021 gcgagcatat tcgggaggtt gccgggcaaa ttgatttccg ggaagccgat ctgctggatc
     1081 tcgaggcctt gcgttccgcc tgtcagggat tggattacgt gctgcaccag gcggcaattc
     1141 cctcggtgcc gcgctcggtg gccgatccgg cacgaagcaa cgcggttaac gtcgacggca
     1201 cgctgaacct gctgatcgcg gcgcgcgacg ccggggtgaa acgcgtggtt tatgccgcct
     1261 cttcgtccgc gtacggtgac acgccgacgc tgcccaagca tgagggcatg ccgcccaatc
     1321 cgatctcgcc ctatgcggta tcgaaactgg cgggcgagct ttacatgact tcgtttcatc
     1381 gcgtctatgg attgcccacc gtcagcctgc gctacttcaa catctttgga ccgcgccagg
     1441 acgcgaactc acaatattca gcggtgctgg ccaaattcat caccagcatg ttgcaaggcg
     1501 agaaatccac gatttatgga gacggcgaac agtcgcgcga cttcacttat attgacaatg
     1561 tggttgcggc caacctgctg gccgcgacgc aacccgaatc gcaggtcgcg ggccagacct
     1621 tcaacatcgc taccggccat cgtttcactc tgaatgagac ctttgccatc ctgaaggaat
     1681 tgactggata ctcaggcgag gttgagtacg caccggcgcg tgcgggagat gtgaaacact
     1741 cgcttgccga cgtctcgcgc gccgaagcgc aactggggta ccggacgctg gtggacttca
     1801 aggaagggtt gcggcgcacc gtcgcctggt atcgcagtgt cgcagtgact gcataagccc
     1861 tgcgccggtg aacggtttgc accgtgctag aatcgcgttt cccaaggaat tacacatgtc
     1921 tttgccatcg aaagctacaa gctccgaatt gtctgaactg ctaaaacgca agatcgaatc
     1981 ccgtaccgcc aaagttggcg tgattggaat gggctacgtc ggcctgcctc tggcgctgct
     2041 attcagcgaa cagaagttcc ccgtaaccgg cttcgatatc gacaagaaga agatcgatgt
     2101 gctggagtcc ggcggatcct acatttaccg gattgcgccc accgagatcc aggcggcgcg
     2161 cacccagggc ttcaccgcga cctcggatta cgacaacatc acccacatgg acgccattat
     2221 tatctgcgtc cccactccac tgaacgagta ccacgagccc gatctcagct acattactgc
     2281 aaccgccgaa tcgattggcc cacatctgcg cgctggccag ttggtcgttc tcgagagcac
     2341 cacatatccc ggcaccaccg aggaggtgct agtaccgatt ctcgagcgct ctaataaacg
     2401 tgggctactg gccgctcgcg cgggcacgga gaacgacaac cggttctacg tggttttttc
     2461 tcccgaacgc gaggacccgg gcaacgacac cgttgcgcgc tgcgatattc ccaaggtcat
     2521 cggcggactc gatccccagg ccggcgaact cggagcgaag ctctacggcg ccatcttcaa
     2581 ccgcacggtt ccggtctcga cacccgcggc tgccgagatg accaagctgc tggagaacat
     2641 ctaccgctgc gtaaatatcg cgatggtgaa tgagttgaag atgctctgcc tgcgcatggg
     2701 catcgacatc tgggaggtga tcgacgcagc caagagcaag cccttcggct tccaggcgtt
     2761 ttatcccggt cccggactcg gcgggcattg catcccgatc gatccgtttt atctgtcgtg
     2821 gaaggcgaag cagttcgatt ttcgtacccg cttcatcgaa ctggccggcg aagtgaacac
     2881 caatatgccg tacttttccg tggaagccgt cgccagcgcg ctgaacaagg agaagaaggc
     2941 gctcaatggc tcgaagattc tagtactggg gctttcctac aagaaggaca ttgatgacct
     3001 gcgggaatcg ccgtcgctca cgattatcga actgctccag aagcgcggcg caatggtcag
     3061 ctacaacgat ccctacttcc cgacggtggg gcatggacgc cgctacgccc tgaacatgac
     3121 gtgtgcgccg ctggagaatt tggaccagta cgactgcgtg gtgatcgtca ccgaccacag
     3181 cgactacgat tatcgtcgaa ttgtgcggga gtcgaagctg gtggtggata cacgcaacgc
     3241 cacgaagggc atcgcctcgc caaagattgt ccgctgctaa ggcgattcca cacttggcgt
     3301 atggggtgga gcaccgcttc agcggtgcag ttaagattcg gaaatgagac ggcttttagc
     3361 cgctgaggtg acggaaacat tacctcaggc gctaaagcgc gatccaagaa ctgacggcag
     3421 ttatgcacgc ctgaaggcgt gctccaccca tcgcctatac accagatgtg aaactgcgat
     3481 agagcggata catcgcgcta tcgccgatag gtccccatgt attccgcctg gattaggacg
     3541 tggcccttta tggcctcctg aagatcgagg ttgtttgcgc cgggcgcgag atcgagtttt
     3601 tcttccaggg catacaagcg gaaaaaatac cggtgcggct tgcccggcgg cggacacggg
     3661 ccgttatagc caatcttcct gaagctgttg cggccctgcc gcgtcccatc gtctaactgg
     3721 tcctgcttcg caactgcctc ggaaagctca tgcgcgctcg ccggcaggtt ccacaacacc
     3781 caatgcaccc atgttccggc aggtgcgtcg gggtcatcca tgatcagcgc gaagctgacg
     3841 gtgcgcgccg gaggcccgcc ccattcgagt gccggcgaaa cgtccgcggc ctcgcaggaa
     3901 tgtttcttcg gaatctctgc gccgtgcgcg aatgcggggc tcttaagcac gagtgccata
     3961 ggtgcacctc ctgcagatgg atatggctgt atcgcaaccg tgctgccgcc aactggaccc
     4021 agcacaagca acagcgcggt gagttttgcg attgaacgaa ggtgcttcag acgggaagct
     4081 gcggaagcgt tgctcg
//
LOCUS       DATMGX010001070         2532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_142777, whole genome shotgun sequence.
ACCESSION   DATMGX010001070 DATMGX010000000
VERSION     DATMGX010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142777"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..1481
                     /locus_tag="VNR65_17565"
     CDS             369..1481
                     /locus_tag="VNR65_17565"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HWJ20529.1"
                     /translation="MSDNEDKENKTEEPSEKRRSEALERDGGPSSREVGSAAVLLIAS
                     AFLAAGTPGLCTGLAQSLAIFIENPGDWRLDNGEDAVRLCQIVAIIIAKFLGLFVAAV
                     LIAGMVAAFLQNPPRLLMTRLAPDFSRVSPSAGLGRLISTQALIEFLKGLVKIGVAAF
                     AAYTAIGALTAGLLAIQSPPDAVPELIRHLCLRAAFICTLMTCVIAAADVFWTRRTWR
                     RNLMMTKQELKEEMKQAEGDLALKARLRMIARSRIRRRMMANVPKATLVVTNPTHYAV
                     ALRYVRNEDSAPKVLAKGQDQLALTIRQIAKKHKIPIIENKLLARSLFEATEIDQLIP
                     PEFYKAVAELIIYLDSKSRKPGQKPGTGRQKNLPPD"
     gene            1563..1946
                     /locus_tag="VNR65_17570"
     CDS             1563..1946
                     /locus_tag="VNR65_17570"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HWJ20530.1"
                     /translation="MAINNVLDLAARQASWLLSRENQVAQNVANANTPGYKTADLTPF
                     EASLQTASLQLTTTSPRHISIPQSEFQITPEENTPEKGDTFLSGNNVSLESEMMKGGE
                     INRAYSLNAAVVKAFNSMIVQSAKG"
     gene            1952..2365
                     /gene="flgC"
                     /locus_tag="VNR65_17575"
     CDS             1952..2365
                     /gene="flgC"
                     /locus_tag="VNR65_17575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HWJ20531.1"
                     /translation="MDNLTATFSVAGSGLFAQSQRIRIASENLANAESTGNSPGANPY
                     TRKVISFESELDEASGADLVQVGDTELDSSPYRLEKMPGNPAADQSGYVKLPNVNMLV
                     ELSDIREATRSYDANVQVVKQTRDLVTMTIDLLKG"
BASE COUNT          597 a          718 c          672 g          545 t
ORIGIN      
        1 cttgggcgtg gcgctgttat ccctctcgac cggagggtcg gagagcctgt cgacgtgatg
       61 atcaacggcc ggattatcgc tcgcggagaa atcgtcgtgc tcgatgaaga aggctcgcgc
      121 ttcggcgtta ccctcacgga gatgatcgac aacgccaaat cccaagtcta gatcaacccg
      181 cctgcgatgg gaagcgattg aaggcggaaa agttgatcga ttctaaagtg ttagagcagt
      241 ttattcgcgt cagatgaacg tggaccacta gaatctttcg ccctctagta gagtttttga
      301 atttgacatt cgagcgcaaa ctcgcggcgg ggaactcatc cacggttgcg cacgagtaaa
      361 ggacgtcttt gtccgacaac gaagataaag aaaacaagac agaggagccg agcgagaaac
      421 gccggtcgga ggctcttgag cgggacggcg ggccttcctc cagggaagtg ggctcagctg
      481 cggtcctcct catcgcttct gcctttttgg ctgccgggac tcctggtctt tgcacgggtc
      541 tggcccaaag cctcgctatc tttattgaga atcccggcga ttggcgactc gacaacggcg
      601 aggacgccgt ccgcctctgc caaatcgttg ctattatcat tgccaagttt ctcgggttat
      661 tcgttgccgc cgtccttatt gctggaatgg tagctgcatt tcttcagaac cctcctagac
      721 tcctgatgac ccgactggca ccggattttt ctcgtgtatc gccatccgcc ggtcttggcc
      781 gtctgatcag cacgcaggcc ctgattgaat tcctcaaggg gctggtcaag atcggcgtgg
      841 ccgcgtttgc ggcctatacc gcgataggcg cccttacggc cggattgctt gctattcaaa
      901 gcccacccga tgccgttcct gaactgatcc ggcatctttg cctccgcgca gcgttcatct
      961 gtactctgat gacctgcgtg attgctgcgg ccgacgtctt ctggacccgt cgcacctggc
     1021 ggcgcaacct catgatgacg aagcaggaac tcaaggagga gatgaagcag gccgagggag
     1081 atctggctct gaaggcgcgc ctgcgtatga ttgcccgatc gcgcatacgg cgccgaatga
     1141 tggcgaatgt gccgaaggcg acgctggttg tcaccaaccc cacccactat gcggtcgcgt
     1201 tgcgctatgt caggaatgaa gattcggctc ctaaggttct ggccaaaggt caagatcaac
     1261 tggccctcac aatacgccaa atagcaaaaa aacataagat tccaattatt gagaacaagt
     1321 tgctggcgcg ctcgctcttt gaagcaaccg aaattgatca acttattcca ccagaatttt
     1381 ataaagctgt tgcagaactt attatctatc tggattccaa atcccggaag ccggggcaaa
     1441 aaccgggcac gggccggcaa aaaaacctgc cgccagactg acaagcccca cgcaagaata
     1501 ataccttaac aagcggttaa cgtgcctgtt cctggcgacg acctcagcaa atggatgctg
     1561 atatggcaat aaacaatgtc ttagatcttg cagctcgcca agcgtcgtgg ctcctctcaa
     1621 gagagaacca agttgctcag aatgtggcga atgcaaacac tcccggctat aaaactgccg
     1681 atttgacacc cttcgaggcg tccctacaga cggcctcact ccagcttacg acgacctcgc
     1741 ccaggcatat cagcatccct caaagcgagt tccaaattac gcccgaagag aacaccccag
     1801 aaaaggggga tacttttctg tccggcaata acgtcagcct ggaatcggag atgatgaagg
     1861 gaggcgaaat caatcgcgct tactcgctga atgctgccgt tgtgaaagca tttaacagca
     1921 tgatcgtgca atcggcgaag ggctagtcgt gatggacaat cttaccgcaa cattttcagt
     1981 ggccggctct gggctctttg cccaatcgca acgcattcga atcgcttcgg aaaatcttgc
     2041 caatgccgag tcgaccggca actcccccgg cgcgaaccca tacacgcgaa aggttatctc
     2101 ctttgagtcg gagcttgatg aagccagcgg agcggacctc gtccaggttg gcgataccga
     2161 actggacagt tcaccctatc gcctggaaaa gatgccaggc aatccggcgg cagaccagag
     2221 cggctacgtc aaactcccga acgtcaacat gctggtggag ctttcggata tccgagaggc
     2281 gacgcggtcc tatgacgcga atgtgcaagt cgtcaagcag acgcgcgacc tggttaccat
     2341 gaccatcgac cttttgaagg gctagcaaat ggcaatcgat ccactctctt ccctttccag
     2401 ttttagcccg cttggcgccg cgcagtcaaa aaagccgctg acttccagcg ctccttccgt
     2461 gaatgcaggc gattttgcga gcgtcttgag cgatctggcc aagcaaaccg cctccaccct
     2521 caaggccagt ga
//
LOCUS       DATMGX010001071         2898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_58818, whole genome shotgun sequence.
ACCESSION   DATMGX010001071 DATMGX010000000
VERSION     DATMGX010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58818"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="VNR65_17580"
     CDS             <1..1241
                     /locus_tag="VNR65_17580"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWJ20532.1"
                     /translation="DARLLAADVMVLGGLNEGSWPKIGAPSPWLNRRDRVFVGLSPEE
                     RRIGLAARDFTALAATGSCVFLTRAKKVNGSLTRPSRWIARLKALATGAGKLETLQPE
                     NPWLSWVRLHRAPTKVTPVDCPTPRPPFAARPRRLSVTAIETWFANPYAIYARHILGL
                     EPLRRAGETHDARDKGILYHAALHRFFQTHPGPLPADAAEKLVRCLDRAAEELGFNLE
                     NAPFWRPRFARFADWFTSTEGNRRAGIVALKSEVGGKLKLDAAAGPFEVTARADRIDI
                     CHDGAVRIYDFKTSANAAKLSAARGAPQLALEGLLVKEGGFAGISPGSEPELRYIVAT
                     GGEPPGEIVELKVPCGAAIQAAATGLALRIARFDDPATPYAYEIRAIFRDKADNDPYA
                     HLARVKEWSAGWDEGEAGDD"
     gene            1234..>2898
                     /locus_tag="VNR65_17585"
     CDS             1234..>2898
                     /locus_tag="VNR65_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWJ20533.1"
                     /translation="MTELSPQELLDAAERKQRAAADPSVSAWVRANAGTGKTHVLVQR
                     ILRLLLSGSAPRSILCLTFTKNAAAEMETRVLSKLGEWATADRETLTANLARLLGRLP
                     SDAERAVAPCLFASVIDAPGGLAIMTIHGFCERVLRRYSFEANVAPGFSVLTEEEARD
                     ALTQAQSQAFASAKDGPLRDALTAVVAYAGETDFGRVLEAMLGRRNEIARLFRLSDGL
                     NPFSGIDARLRHFLGVKNNDTRESLLVRAASLLPERTLRDVVAILFEGTKTDREAAAR
                     FQAVAEVGDAEGKHSALKAAFTTKTGEPRKSLMTASIRQQAPELNDRLVKAQAAFVAL
                     DERICGLKVVEATMALLRLTIAIFSRYDAEKRARAAVDFDDLIDKTLSLLSREEATDW
                     VLYQLDGRIDHILVDEAQDTSRAQWEIIDRLTSDFFSGEGARNCLPTLFAVGDEKQSI
                     YGFQGAAPELLARHGASYEEKAQQAGFLWRTVDLNLSFRTLAPVLAAVDSVTAALPGL
                     VHGSSLPHVAHRHKGGGLVELWEPERGEKVDKGTVWETDAAPGPAAK"
BASE COUNT          540 a          904 c          898 g          556 t
ORIGIN      
        1 tcgatgctcg cctgcttgcc gccgacgtga tggtgcttgg cggattgaac gagggaagct
       61 ggccgaaaat cggcgctccc agcccctggc tcaatcgcag ggatcgcgtt tttgtcgggc
      121 tttcgccgga ggagcgccgc atcggcttgg ccgcccgaga cttcaccgcg ctcgccgcca
      181 ccggctcttg cgtctttctt acgcgcgcca agaaggtgaa cggcagcctg acccggccca
      241 gccgctggat tgcacgcctg aaagcgctcg ctaccggggc cggcaaactg gaaaccttgc
      301 agcctgaaaa tccgtggctt tcctgggtgc gtcttcaccg agcgccgaca aaagttacgc
      361 ccgtggattg tcctactcct cggccaccgt tcgcggcccg tccacgccgc ctgagcgtga
      421 ccgcaatcga gacgtggttc gccaatcctt acgcgattta cgcacggcat attcttggcc
      481 ttgagccttt acgccgggcc ggagagaccc atgacgcgcg cgacaagggc attctttatc
      541 acgccgcgct gcatcgtttc ttccaaactc atcctggtcc cctgcccgcg gatgcggcgg
      601 agaaacttgt gcgatgcctg gacagagcag cggaggagct aggcttcaat ctcgaaaatg
      661 ccccgttctg gcgcccgcgt ttcgcgcgct ttgccgactg gtttacaagc accgagggca
      721 accgccgcgc cgggattgtc gcattaaaga gcgaggttgg tggaaaattg aagctcgatg
      781 cagcggcggg acctttcgag gtgactgcgc gcgcagacag gatcgacatc tgccatgacg
      841 gcgcggtacg catttacgac ttcaagacca gcgcgaatgc ggcaaaactc tcggctgcgc
      901 gcggcgcgcc gcaacttgcg ctcgaaggcc tgcttgtaaa ggagggcggc tttgccggga
      961 tctccccggg gtctgagccg gagcttcgtt acatcgtcgc taccggcggc gagccgccgg
     1021 gcgaaatcgt tgagttgaag gtgccgtgtg gagcagcgat ccaggcggct gcaaccggtc
     1081 tggcgctccg cattgctcgc ttcgacgatc ccgctacgcc ctatgcttac gaaatacgcg
     1141 ctatctttcg cgataaggcg gacaacgatc cctatgctca ccttgcccgc gtgaaagagt
     1201 ggtccgcggg ttgggacgag ggggaggcgg gcgatgactg agctatcgcc tcaagagctg
     1261 ctcgacgccg ccgagcgcaa gcaaagggcg gcggccgatc cctccgtctc ggcctgggtc
     1321 cgagcgaatg ctgggactgg caagacccac gttctcgtcc agcgaattct gcggctgctt
     1381 ctttccggct cggcgccccg gtcgatcctc tgcctgacct tcacgaaaaa cgccgcggcc
     1441 gagatggaaa cgcgcgttct ctctaagctc ggcgaatggg ccacggcaga tcgagaaacg
     1501 ctaaccgcca atctggcgag gctgctcggc cggctgccaa gcgatgccga acgggccgtc
     1561 gccccctgcc tctttgcgtc tgtgatcgat gcaccgggcg gtctcgccat catgacaatt
     1621 cacggctttt gcgagcgcgt gttacgccgc tacagctttg aagccaatgt ggcacccggt
     1681 ttctccgtgc tgacggagga ggaagcgcgc gacgcgctca cgcaagcgca gagccaagcc
     1741 tttgcatcgg ccaaggatgg gcctttgcgc gatgccttga ccgcggtcgt cgcctacgcg
     1801 ggagaaaccg atttcggccg cgtgcttgag gccatgcttg ggcgccgaaa cgagatcgcc
     1861 cgtctcttcc ggctttccga tgggcttaat cctttcagtg gcatcgatgc gagacttcgc
     1921 cacttcctcg gggtcaaaaa caatgacacc agagagagcc ttctggtccg cgctgcttcg
     1981 ctgctacccg aacgcacact gagagacgtt gtcgccatcc tcttcgaggg cacgaaaacc
     2041 gatcgcgaag cagcggcacg gttccaggcc gtagctgaag tgggcgacgc cgagggaaaa
     2101 cattcagcgc tgaaggctgc attcacgacg aagaccggag agcctcgaaa gtcccttatg
     2161 acggcctcga tcagacagca ggccccggag ctgaatgatc gccttgtgaa ggcgcaggcg
     2221 gcttttgtcg cactcgatga acgaatctgc ggtctcaaag tcgttgaggc cacgatggcc
     2281 cttctgcgcc tcacgatagc gatctttagc cgctacgatg ccgagaagcg cgcgcgcgcc
     2341 gctgtcgatt tcgacgacct catcgacaaa accctgagcc tgctatcccg agaggaggcg
     2401 accgactggg ttctctatca gctcgatggg cggatcgatc atatccttgt cgatgaagcg
     2461 caggatacaa gccgcgcgca gtgggagatc atcgacagac tgacttcgga tttcttctcc
     2521 ggcgaaggcg ctcgcaattg cctgccgacg ctgttcgcgg taggcgacga gaagcagtcg
     2581 atttatggct ttcagggcgc ggcccctgag ctgctcgccc gtcatggcgc ttcttatgag
     2641 gagaaggctc aacaagccgg ctttctctgg cgcactgtcg acctcaatct gtccttcaga
     2701 acactcgcgc ctgttcttgc cgcggtggac agcgtgaccg ctgctctgcc tggcctggtg
     2761 catggttcgt ccctgcccca tgtggcgcat cggcacaaag gcggagggct cgtagaactg
     2821 tgggagcccg aacgaggaga aaaggtagac aagggaacgg tctgggaaac agatgccgcc
     2881 cctggcccgg ctgcaaag
//
LOCUS       DATMGX010001072         5764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_213617, whole genome shotgun sequence.
ACCESSION   DATMGX010001072 DATMGX010000000
VERSION     DATMGX010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213617"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VNR65_17590"
     CDS             <1..815
                     /locus_tag="VNR65_17590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWJ20534.1"
                     /translation="CFTSSGRFPTFEAVSLQRLGMEFFSSDGVKIAFAVEGEGEPILL
                     IHGFASNMQTNWSGTGWIRLLSMNGFRVIAFDHRGHGASEKLYNPAAYTGPTMAEDAR
                     RLLDHLGIEQADVLGYSMGARIAAFLGLRHPERVRSAIFAGLGANMVFGIGDPKPIAE
                     ALLAEDASLVTDANARAFRIFADQTKSDRRALAACITAARDRIPGHEIAKFQRPVLVA
                     VGTNDALAGPPKPLADAIPGAEALDIPGRDHMKAVGDRVFKEGVLSFLERRP"
     gene            888..1751
                     /gene="cysE"
                     /locus_tag="VNR65_17595"
     CDS             888..1751
                     /gene="cysE"
                     /locus_tag="VNR65_17595"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HWJ20535.1"
                     /translation="MLNTKHRGKSAKVSAYDPVWDQIRAEAEDVTHREPELTAFIFAN
                     ILNHSQLEHSVCHRLAQRLGHDDVDADLMRQTFLSIVESHPEFGTMFRVDVAAVYDRD
                     PACNRLIDPLLYFKGFHALETQRFSHALWHLGRKDFALYLQSQSSRVFSVDIHPAAKI
                     GRGIMFDHGTGIVIGETAEIGDNTSLLHNVTLGGSGKETGDRHPKVGRGVMIGAGAKI
                     LGNIVVGDCARIAAGSVVLKDVPPRTTVAGVPARVVGEAGCSQPSRVMDQLLKGMELG
                     RPETREPTGEA"
     gene            1748..1960
                     /locus_tag="VNR65_17600"
     CDS             1748..1960
                     /locus_tag="VNR65_17600"
                     /inference="COORDINATES: protein motif:HMM:NF022768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HWJ20536.1"
                     /translation="MTPAEIKSLEKYLRKTFSLEAIEVKKRAKKDDSVEVFVKEEFIG
                     VIYKDDEDNEVTYQFQMAILADDLKD"
     gene            1972..2505
                     /locus_tag="VNR65_17605"
     CDS             1972..2505
                     /locus_tag="VNR65_17605"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWJ20537.1"
                     /translation="MPILALDGVSPKLPPIGDFFIAPSAYVIGKVSLGRGVSVWYGSV
                     LRGDNELLEVGDETNVQEHCVFHTDEGVPFKVGRGCTIGHRALLHGCRIGDNCLIGIG
                     ATIMNNAVIGDNCLIGAHTLITEGKTIPAGSVVMGSPGKIIRTVSPEEIAGFRASAHH
                     YIEASRRFLTLLSEGDV"
     gene            3151..3582
                     /locus_tag="VNR65_17610"
     CDS             3151..3582
                     /locus_tag="VNR65_17610"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWJ20538.1"
                     /translation="MTVAWILKDKGRHVVSVLSGTPVTEAIGHLEKNRIGAVVIVDEQ
                     HRVLGILSERDIVRILATRGIGALSEPVSQHMTHPVSTCSEHHSIDWVMNEMTNHRFR
                     HIPITDDGRLAGIVSIGDVVKLKLALAESEAAQMRQYFSAG"
     gene            complement(3602..4546)
                     /locus_tag="VNR65_17615"
     CDS             complement(3602..4546)
                     /locus_tag="VNR65_17615"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWJ20539.1"
                     /translation="MAKTLIGLALGGGAARGLAHIGVIEALMEAGLEPDIIAGTSIGA
                     IAGGFYASGRLDILHSLARELTTRKLLRYLDVNLAGSSLVSGKRLEKTLASHIGDIHI
                     ETLPKKFVAIATELGTGQEVWLTSGLLTAALRASYALPGIFKPVRIGGRWLMDGALVN
                     PVPVSAARALGANFVIGVTLHTGHVRNTLFPIEAEAAPDPKPERVYDEDGEKKSRFAL
                     VKRQMFGGRRRDGAAPGIPRVVLEAFNITQNRIARSRLAGDPPDATIQVHLDGVGLFD
                     FHKAEYAIEQGRLAMRRFLHSMRRDGIAIPTADLAARR"
     gene            complement(4683..5078)
                     /gene="hisI"
                     /locus_tag="VNR65_17620"
     CDS             complement(4683..5078)
                     /gene="hisI"
                     /locus_tag="VNR65_17620"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HWJ20540.1"
                     /translation="MIGGSFQSPSSNKSELEEGAAFTRRFDVDGLIPALITDAEGGSV
                     LMFAYMNAEALRLTIETREAHFWSRSRKSHWRKGETSGNRLLVQDLLTDCDQDVIWVK
                     AKLSGKAACHTGRPSCFYRRIRADGRARS"
     gene            complement(5075..>5764)
                     /gene="folE"
                     /locus_tag="VNR65_17625"
     CDS             complement(5075..>5764)
                     /gene="folE"
                     /locus_tag="VNR65_17625"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWJ20541.1"
                     /translation="SYRLPKGYVPMDTRASVTGPITREQNGHRVPALTKRPTREEAEA
                     AVRTLIAWAGDDPAREGLLDTPGRVVEAYEEFFAGYDGDPYGALDRTFQDVAGYDDLV
                     MVRDIRLESHCEHHMLPIIGVAHVAYLPSGSVVGISKLARVVDLYAKRLQTQETMTAQ
                     IANAIEEVLKPRGVAIMIDAIHECMTTRGVHKPGTTTITTQFTGVFKDDPSYRERFMQ
                     LVNGVRPIGMK"
BASE COUNT         1211 a         1720 c         1666 g         1167 t
ORIGIN      
        1 tgtgctttac ttcttcagga cggtttccga cattcgaagc ggtatctttg caaaggcttg
       61 gcatggagtt cttctcttcc gacggcgtga aaatcgcttt tgcggtggag ggtgagggag
      121 agccgatcct tctcatccat ggctttgcct cgaacatgca aacgaactgg agcgggacgg
      181 ggtggatcag gcttctgtcc atgaacggct tccgggtgat cgcgtttgac catcgcggcc
      241 acggcgccag cgagaagctc tacaatccgg cagcctatac cggcccgaca atggcagaag
      301 acgcgcggcg gcttctcgat catcttggga tcgaacaggc ggacgtgctt ggctattcca
      361 tgggcgcgcg catcgctgcc tttcttgggc tccggcatcc cgagcgcgtt cgcagcgcca
      421 tctttgctgg gctcggcgcg aacatggtgt ttggcattgg ggacccgaaa ccgatcgcgg
      481 aagcgctact ggcggaggat gcctcgcttg tcaccgatgc gaatgcgcgg gcctttcgca
      541 ttttcgccga ccagacgaaa agcgaccgcc gcgcgctcgc agcctgtatc acggccgccc
      601 gcgaccgcat cccgggccac gagatcgcca agttccagcg tccggtcctt gtcgcggttg
      661 gcactaatga cgcgcttgca ggtccgccga agccgctggc cgatgcgata ccgggagctg
      721 aagcattgga tattccaggc cgcgaccaca tgaaagcggt gggcgaccgg gttttcaagg
      781 aaggcgtact ctcttttctt gaaaggagac cataaacgcc aaatctttgc gcatgaacgc
      841 ttattcagtt aagctcaacg aaccccaccc ccggagatca ttcgacgatg ttgaacacga
      901 aacaccgagg caaatccgcg aaggtctcgg cctacgatcc ggtgtgggat caaattcgcg
      961 ccgaggccga ggacgtaaca caccgggagc cggagctgac ggcctttatc ttcgcgaata
     1021 tcctcaatca tagccagctt gagcattcgg tctgccatag gctggcgcag cggcttggcc
     1081 acgatgatgt ggacgccgac ctcatgcggc agacgttcct gagcatcgtc gaaagccatc
     1141 cagaatttgg aacgatgttc cgagtggatg ttgcggcggt ttacgatcgc gatcccgcct
     1201 gcaaccggtt gatcgacccg cttttatatt tcaagggctt tcacgcgctc gagacccaac
     1261 gatttagcca cgcgctttgg caccttggcc gaaaggattt tgcgctctac ctgcagagcc
     1321 aatcctcgcg cgtgttcagc gtcgacattc atccggcagc aaagatcggg cgcgggatca
     1381 tgttcgacca cggcaccggg atcgtgatcg gagaaaccgc cgagattggg gacaacacct
     1441 cgctcctcca taacgtcacg cttggtggca gcgggaaaga gacgggcgac cgccatccca
     1501 aggtcggacg aggcgtcatg atcggggccg gcgccaagat cctcggcaac atcgtcgtcg
     1561 gcgattgcgc gcgcatcgct gcgggaagcg tggtgcttaa agacgtcccg ccgagaacaa
     1621 cagttgcggg cgtgcctgcc cgggtggtag gcgaagcagg ttgcagccag ccatcccgcg
     1681 ttatggatca actcctcaag ggaatggagc ttggcagacc ggaaacacgt gaaccgacag
     1741 gcgaagcatg acccccgcag aaatcaagag ccttgaaaag tatttgagaa aaacattcag
     1801 cctcgaggca atcgaggtca aaaaacgagc caagaaggat gattccgtcg aggtgttcgt
     1861 aaaggaggaa ttcatcggcg tcatctacaa ggacgatgaa gacaacgaag tcacttacca
     1921 atttcagatg gccatcctgg ccgacgattt gaaagactga cgggatttgc catgcccatt
     1981 ctggccctcg atggagtgtc cccaaagctt ccacccatcg gcgatttttt tatcgcccca
     2041 agcgcttatg tcatcggcaa ggtctctctg ggccgcggcg tctccgtttg gtatgggtcc
     2101 gtacttcgtg gcgacaacga gcttctcgaa gtaggagatg agaccaatgt tcaggagcat
     2161 tgtgtctttc acaccgacga gggcgtccca ttcaaggttg ggcgcggctg caccatcggg
     2221 caccgcgctc ttctccacgg atgccggatc ggcgacaatt gccttatcgg catcggcgcc
     2281 acgatcatga acaacgccgt gatcggcgat aactgcctga tcggggctca cacgctcatc
     2341 accgaaggaa aaacgatccc ggcggggtcg gtcgtgatgg gctcacccgg aaaaatcatc
     2401 cgcaccgtct cgccggagga aatcgcaggt tttcgcgcct ctgcgcacca ctatatcgag
     2461 gcctcccgcc gtttcctcac cctgctatca gagggcgacg tttaagttcg ccctgcacaa
     2521 tcgtttttga atcgcccgtc atgtggcacg gaacgctgcg gctgcgttga ttaaatcgcc
     2581 tgcgacgcga tgcgtcattc tgaccacttt tcattattaa ccagaggcgt agcctaatag
     2641 ttgtccagca aggacaagcg agcgccgatg ctattcgagc accgagtgta taaaaagcag
     2701 cgatcgggcg ctcgctttac ccttctcctc cggaaataat tgtcctttac ttggcagctc
     2761 cctctgctag ctagcggctt agcaggtacc aaagtaatcc tccggttgag aacttaatct
     2821 aagtcacact caaagcccgt cggctaactc ggaagaaagc gatgccgcaa aagaaaggcc
     2881 ggagcttttg gccccggcct tcgctgtggc ccggcgggac gggacatggg ggcaagggag
     2941 gccttgccac agcgtttcat ctgtgaaaac cggcatcagg ctattgggtt ccctgtcagg
     3001 gcaagattcg cgttcacgac gttccgcgtc ccagttgcgc aaaggtctct ttacgcgcat
     3061 cattaagaaa aaggctgttg aactgatcga cgcaccaggc gccgcaaggg cgaacagcgc
     3121 catgaggcag tttcgcttgg gaggataaga atgacggttg catggatttt aaaggacaag
     3181 gggcggcatg tggtcagcgt tttgtctggc acgccggtca ccgaagccat cggccacctc
     3241 gaaaagaatc ggatcggtgc agttgtcatc gtcgacgaac agcaccgcgt gctgggcatt
     3301 ttatctgaac gggatatcgt gcggatacta gcaacgcgcg gaataggggc ccttagcgag
     3361 cctgtaagcc aacacatgac gcatccggtt tcaacctgct ccgaacacca cagcatcgac
     3421 tgggtcatga acgagatgac gaaccatcgt tttcgtcata ttccgattac cgatgacggg
     3481 cgtctagcgg gaatcgtctc catcggcgac gttgtgaagc tgaagcttgc gctcgcggag
     3541 tcggaagctg cgcagatgcg ccagtacttc tctgcgggct agccttccct gcatacgacc
     3601 cctaccgccg tgcagccagg tccgccgttg gaatggcaat accatcgcgg cgcatcgagt
     3661 gaagaaagcg ccgcatggcc aagcggcctt gctcgatcgc atattcagcc ttgtgaaagt
     3721 cgaacaggcc gacgccgtct aggtgaacct ggatcgtagc gtctggcgga tctccggcca
     3781 gccttgagcg cgcgatccgg ttctgagtaa tgttgaaagc ctcgagcacc acccgcggaa
     3841 tcccgggcgc ggcgccgtca cgccgccgtc ctccgaacat ctgccgcttc acaagagcaa
     3901 aacggctctt cttttcccca tcctcgtcat aaacacgctc gggcttgggg tcgggcgcgg
     3961 cttcggcttc gatggggaaa agcgtgttgc ggacatggcc ggtgtgaagg gtcacgccga
     4021 tgacgaagtt ggccccaagc gcacgcgcag cactcacagg cacagggttg acgagcgctc
     4081 cgtccatcaa ccaccgccca ccgatgcgca ccggcttgaa aattccaggc aaggcatagg
     4141 aggcccgcag cgccgccgtg agcaatccgc tcgtcagcca gacttcctgg ccggtgccca
     4201 gctctgtcgc gatggcaaca aactttttcg gaagcgtttc gatatggatg tcgccgatgt
     4261 gcgacgcgag cgttttttcc aggcgcttgc cggacacgag gctcgacccg gcgagattga
     4321 cgtcgaggta gcgcaacagc tttcgcgtcg tgagctcgcg cgctaaactg tgcaggatgt
     4381 caagcctccc cgacgcatag aaaccgcctg caatcgcgcc gatggaggtg cctgcgataa
     4441 tatcgggttc gaggccagct tccatcagtg cctcgatcac accgatatga gcgagcccgc
     4501 gcgccgcccc gccacctaat gccaatccga taagtgtctt cgccatctaa gggctcgtat
     4561 acggtgtcaa acaatctgga aaaagcgcga atacgaagaa atgaaatctg agtctaccac
     4621 gcagcagcaa ccagactttt atgttaaccc gcaacttatg agcgctctcc ttcacaaata
     4681 tttcaagatc gtgccctgcc gtctgcgcga atgcgcctat agaaacaact cggcctgccc
     4741 gtgtggcatg ctgcctttcc gctcagcttg gctttgaccc aaatgacatc ttggtcgcag
     4801 tcggtcagaa ggtcttggac gaggagccga ttgccactcg tctcgccctt ccgccaatgg
     4861 gacttcctcg accggctcca gaaatgcgct tctctggttt cgatggtcag tcgcaacgct
     4921 tcagcattca tataagcgaa catcaagaca ctgccgccct ccgcatcggt gatgagggcc
     4981 gggatgagac cgtccacatc gaagcgccgc gtgaacgccg cgccctcttc aagctccgat
     5041 ttgtttgagg aaggcgattg gaaggagcct ccaatcactt catcccaatc gggcgcacgc
     5101 cgttgacgag ttgcatgaac cgttcgcgat agctcgggtc gtccttgaaa acgcccgtga
     5161 actgcgttgt gatcgttgtc gtgccgggct tgtgcacgcc tctggtcgtc atgcattcgt
     5221 ggatggcgtc gatcatgatc gcgacgccgc gcggcttcag cacttcctcg attgcgttgg
     5281 cgatctgcgc cgtcatggtc tcctgcgttt ggagccgctt cgcgtaaagg tcgacaacgc
     5341 gggcgagctt cgagataccg acaacgctcc cgctcggcag gtaggcgaca tgcgcaacgc
     5401 cgatgatcgg cagcatgtga tgctcgcagt gggactcaag gcggatgtcg cgcaccatca
     5461 cgaggtcgtc gtagcccgcg acatcctgga aggtccggtc aagcgccccg taaggatcgc
     5521 cgtcatagcc agcgaaaaac tcctcatagg cttccaccac gcgccccggc gtatcgagca
     5581 gcccctcgcg cgcagggtcg tcgcccgccc acgcgataag ggtacgcacc gccgcctcgg
     5641 cctcctcgcg cgtgggccgt ttcgtcagcg cgggcaccct atgcccgttc tgctcgcgcg
     5701 tgatcggccc cgtgactgaa gctcgtgtat ccatgggaac ataccctttc ggcaggcgat
     5761 aaga
//
LOCUS       DATMGX010001073         3240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_311784, whole genome shotgun sequence.
ACCESSION   DATMGX010001073 DATMGX010000000
VERSION     DATMGX010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311784"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VNR65_17630"
     CDS             <1..917
                     /locus_tag="VNR65_17630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWJ20542.1"
                     /translation="DGAGRAVTDPSGKVRAGDSFTVDEPAAEPYAVAGEQIELKVVYE
                     DESLIVIDKQPGLVVHPGAGNQSGTLVNALIAHCGASLSGIGGVARPGIVHRLDKDTS
                     GVMVVAKTDAAHRRLAAQFADHGRKGELRRDYLALVWGAPNPRAGRIETLIGRHSSSR
                     MKMTVVSNGGRVAITNYTTEHTFRVPAQKTASKGKGNNSLLLSVVRCSLETGRTHQVR
                     VHMAHIGTPIVGDLVYGAGFRTKASALPENEAQMFMSMERQALHAFSLRLKHPITGKI
                     ETFQSALPEDIAKLCRALQSLEAKLSKN"
     gene            1021..1947
                     /gene="rpoH"
                     /locus_tag="VNR65_17635"
     CDS             1021..1947
                     /gene="rpoH"
                     /locus_tag="VNR65_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HWJ20543.1"
                     /translation="MSSLPSVAASGGLARYLEEIRRFPMLEPQQEYMLAKRWQEHEDA
                     AAAQKLVTSHLRLVARIAMGYRVYGLPIGEVISEGNVGLMQAVKRFDPDKGFRLATYA
                     MWWIRAAIQEYILRSWSLVKMGTTASQKKLFFNLRKAKSQLQALEEGDLRPDQVKLIA
                     TNLGVPEEDVISMNRRLGGDASLNAPLRADSESGEWQDWLVDEGPNQETMLVESEELD
                     RRREYLGAAMDGLNERERRIFTARRLADDPLTLEELSTEFNVSRERIRQIEVRAFEKV
                     QKAVQRAAVKAETPRTSAAAAAAGAPAFSAAP"
     gene            2117..>3240
                     /gene="aceB"
                     /locus_tag="VNR65_17640"
     CDS             2117..>3240
                     /gene="aceB"
                     /locus_tag="VNR65_17640"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HWJ20544.1"
                     /translation="MPNQASPSLSLPKGVTVSGVLKPGYERILTSDALEFVASMARRF
                     EAQRRQFMDARAERQAKLDGGALPDFLPETRKIRENDWKITGIPDDLLDRRVEITGPT
                     TRKMIINALNSGAKVFMADFEDALSPSWANIVEGQINLKDYWEGRIDFTEEETGKRYE
                     VGSNPAVLFVRPRGWHLLEDHITVDGDKIAGAFVDFGLYFFHNAQGILNRGTGPYFYL
                     PKMESHLEARLWNEVFLFAQESLQLRNGTIKATVLIETLPAVFEMHEILYELRDHIVG
                     LNCGRWDFIFSYIKTLRAKPEYVLPDRSQVVMSKAFLKAYSDLLIKTCHKRGAFAMGG
                     MAAFIPNRRDVEVNEKALAAVREDKLREAQNGHDGTWVAHP"
BASE COUNT          705 a          872 c          994 g          669 t
ORIGIN      
        1 gggatggggc agggcgggcc gtgacagacc cgagcgggaa ggttcgcgcg ggcgacagct
       61 tcaccgtcga tgagcccgcc gccgagcctt atgccgtcgc tggtgaacag attgagctca
      121 aggttgttta tgaggatgag agtttaattg taatcgataa acagccgggg ctcgtcgtgc
      181 atccgggagc tggaaatcag agtggaaccc ttgtcaatgc gctgatcgcg cattgcgggg
      241 cgagcctgtc cgggattggc ggggtcgccc gtccggggat cgtgcaccgg ctcgataaag
      301 acacgagcgg ggtgatggtc gtggcgaaga ccgatgcggc ccacaggaga cttgccgcgc
      361 agtttgccga tcatggccgc aaaggtgaat tgcgccggga ttatctagcc ctcgtctggg
      421 gcgcgccaaa tccgcgtgct ggccggatcg agacgttgat cggaagacac tcctcgtcgc
      481 gtatgaagat gacggttgtg tcgaatggag gccgtgtcgc catcacaaat tacacgaccg
      541 aacacacctt tcgggtgccc gctcagaaga ctgcgtcaaa ggggaagggg aacaattcgt
      601 tattgctaag tgtcgttcgg tgtagcctcg agacagggcg cacgcatcaa gtccgggttc
      661 acatggctca tatcggtacc ccaatcgtgg gagatctcgt ttatggagcc ggtttcagga
      721 cgaaagcttc cgccctccct gaaaacgagg cgcaaatgtt tatgagcatg gaaaggcagg
      781 ctctccatgc gttcagctta cggttaaagc atccgattac cggaaaaatt gaaacgtttc
      841 agtcagcttt gcctgaagat atcgcgaaat tgtgtagggc acttcagagc ctggaggcaa
      901 aactgtccaa gaattaagat gcacacggtt tataggcagg gtaatgtgag ggtcgtcgtt
      961 caaagaaaac gaacgagttc tccaacgagc agcggacttt tcgctgcgga gggtagatgc
     1021 atgagttcat tgccgtcagt tgcagctagc ggtggtctgg cccgttatct cgaggagata
     1081 cggcgcttcc ctatgcttga gcctcagcaa gaatacatgc ttgccaagcg ttggcaagag
     1141 cacgaagatg ccgcagccgc acagaagctg gtgacgtcac atttgcgcct cgttgcccgc
     1201 atcgcaatgg gctacagagt ctatggcttg ccgatcggcg aggtgatctc cgagggcaat
     1261 gtcggcctca tgcaagccgt gaaacgcttc gatcccgaca agggcttccg ccttgctacc
     1321 tacgctatgt ggtggatacg cgctgcaatt caagaatata ttctgcggtc ctggagcctt
     1381 gttaagatgg gcacgaccgc aagccagaag aagcttttct tcaatctgcg caaggcgaag
     1441 agccagctgc aggcgcttga ggaaggcgat cttcgtcccg accaggtgaa gctgatcgcc
     1501 accaatctcg gcgtgcctga ggaagacgtt atttcgatga accgccggct tggcggcgac
     1561 gcttcgctga atgctcccct gcgcgcggac tctgagagcg gcgagtggca ggattggctc
     1621 gtcgatgaag gccccaatca ggagacaatg cttgtcgaga gcgaggaatt ggaccgccgg
     1681 cgggaatacc tcggagcggc aatggatggt cttaacgagc gcgagcgccg tatctttact
     1741 gcgcgccgct tggctgacga ccccctgacg ctcgaagagc tgtcgacgga attcaatgtc
     1801 agccgcgagc gcattcgcca gatcgaggta agggctttcg agaaggtgca aaaggccgtt
     1861 cagagggctg ccgtgaaggc cgaaacgccg cgcacttccg ctgctgctgc cgcagcgggc
     1921 gcacccgcat tttcagccgc cccatagagt ttcttgcctg gctagggctt gaagccagcg
     1981 ctcatgcgct ggcttttttt tgttacaatt gtagaagaat ttattcgggc tcaggtgtta
     2041 cagcctatgg acaccgccga aatccttgtc cttttatacc gtcagtcgaa gctcgacccg
     2101 aaagggaagg aaagtcatgc ccaatcaagc ctctcccagc cttagccttc ccaagggcgt
     2161 cacggtttcc ggagtcttga agcccggcta tgagcgcatt cttacaagcg atgcgctcga
     2221 gtttgtggcg agcatggcac ggcgcttcga ggcccagcgc aggcagttca tggatgcgcg
     2281 cgctgagcgg caggcgaagt tggacggcgg cgctttgccc gacttcctgc ccgagacccg
     2341 caagatccgt gaaaacgact ggaagattac cggcatcccc gatgacctgc tcgacaggcg
     2401 cgtcgagatt accgggccga caacgcgcaa aatgatcatc aacgcgctta actcgggcgc
     2461 gaaagtattt atggccgatt tcgaggatgc cctttcgccg agttgggcga acatcgtgga
     2521 aggccagatc aatctgaaag actattggga agggcggatc gacttcaccg aggaagagac
     2581 gggcaagcgc tatgaggttg gctctaaccc agccgttctg tttgtgcggc cgcgaggctg
     2641 gcacttgctc gaggaccaca tcaccgttga cggcgacaag atcgcaggtg ctttcgtgga
     2701 tttcggcctc tacttcttcc acaatgcgca aggtatcctg aaccgcggga cggggccata
     2761 cttctatctt ccgaagatgg aaagccatct cgaagcgcgg ctctggaacg aagtgttcct
     2821 gtttgctcag gagagcttgc aactccgcaa tgggacgatc aaggccaccg tgctgatcga
     2881 gacgctgccc gcagtcttcg agatgcacga aattctctac gaactgcgcg accacatcgt
     2941 cggcctgaat tgcgggcgct gggactttat tttctcttac atcaagacac tgcgcgcgaa
     3001 gccggaatat gtcttgccgg atcgaagcca ggtcgtgatg agcaaggctt ttctcaaagc
     3061 ctattcggat ctcttgatca agacctgtca taagcgcggc gctttcgcca tgggcggcat
     3121 ggccgccttc attccgaacc ggcgcgacgt ggaagtgaac gagaaggcgc ttgctgccgt
     3181 gcgcgaggac aagctgcgcg aggcgcagaa tggccacgac gggacatggg ttgcccatcc
//
LOCUS       DATMGX010001074         3345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_363416, whole genome shotgun sequence.
ACCESSION   DATMGX010001074 DATMGX010000000
VERSION     DATMGX010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363416"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..706
                     /locus_tag="VNR65_17645"
     CDS             113..706
                     /locus_tag="VNR65_17645"
                     /inference="COORDINATES: protein motif:HMM:NF025703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HWJ20545.1"
                     /translation="MKPRVWLAAILVCLLQTGAVAALIVNRAMLLTHGREIVLSVVPV
                     DPRDLLRGDYVRLNYAVSQIPASLVQASGRQDTGRSIFVTLERQGQPEDGRWVPVAAS
                     PEPPAAPEAENKIVLHGTMSLPWASLASDGLYRNAAAVRVKYGIESYFVPENTGSDLE
                     QATRTGAVKAVISVDRDGNAAIKGLIANGTRHDETLF"
     gene            complement(892..1236)
                     /locus_tag="VNR65_17650"
     CDS             complement(892..1236)
                     /locus_tag="VNR65_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HWJ20546.1"
                     /translation="MSTLPPCPKCNCEFTYEDGGLCVCPECAHEWNAGAATPDDEAGG
                     RVYRDSAGNTLRDGDTVIVIKDLKLKGSSGVVKMGTRVKNIRLVDSDHDIDCKIDGIG
                     AMSLKSEFVKKA"
     gene            complement(1467..>3345)
                     /locus_tag="VNR65_17655"
     CDS             complement(1467..>3345)
                     /locus_tag="VNR65_17655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HWJ20547.1"
                     /translation="SSSIPAAIISMSVLRALTSGGKPAGILENNIVQTQASAAGTLCN
                     VILVLPGLLLIGFWHDFPLWQTMAVCLLGGLLGVAYSVPLRRVMVVGSSLPFPEGVAA
                     AEVLRAGHKDEEKTGEAGSEAGLKELGLGAAAAAGLTLLTTGFKFLPDKLSGSISYGG
                     AVFQMGGSLSLALIGVGYLVRIGVCLALFLGLFIAWGVAVPVLMTIEPSSAGNPVAAA
                     DAVWSEKVRLIGAGIIASGGLWTVLTLIKPLVDSVRLALQAHKHHGANMPREENDIPI
                     AWIGGTIAVLCILTGGLFAWFVSGVHLAANLYWVVPAVTLLTMSLGLLMASACGYLAA
                     LLGSSCSPISGIGILSTILIAAGFGFVFPMGNGEESRAVVALTLFIASIVVTVSSIAN
                     DNLQDLKTGNMVGATPWKQQVALAIGVVAGAITIVPVLQLLYSNYGFVGSLPRAGMLA
                     ENAMAAPQASLMTQIANGIIHHQLAWNMIGIGAALGVALVALESWLWRKRGFSLPALT
                     VGIGIYLPYEVSLTIAIGGVIGWFSERAVAKRAANRDAQGKAEVEARVRRRGVLIASG
                     FLVGESMTGVLLAAADAFGGRSGSLAFSDFAGSHISTAIAALLFASALAVFYRLAAHT
                     S"
BASE COUNT          732 a         1023 c          971 g          619 t
ORIGIN      
        1 gcgatctcat caacacctcg ctattcttcc tggccgctgg cgccggcatc ttcggcctcg
       61 cctgggttct catgcgctat ggcgctgcgc cgcgtgaaaa ggaggcacag tcatgaagcc
      121 gcgcgtttgg cttgccgcca tcctggtctg ccttctacaa accggtgccg tcgcggccct
      181 gattgtcaat cgcgccatgc tgctgaccca cggacgcgaa atcgtgctca gcgtggtgcc
      241 ggtcgatccc cgagacttgc tccgcggcga ttatgtgcgc ctcaactatg cggtctctca
      301 gattcccgcc tcgctcgtgc aggcatcagg acgtcaagac acgggcaggt ccattttcgt
      361 gacccttgag cgccagggcc agccggagga cgggagatgg gttccggtgg ctgcctctcc
      421 ggagccccca gccgcgccgg aagcggaaaa caagatcgtt ttgcacggga ccatgagctt
      481 gccctgggct tcgctggcct cggacggatt ataccgcaat gctgctgccg ttcgcgtgaa
      541 gtacggcatc gagagctatt tcgtacctga gaacacgggc agcgatctgg agcaggcgac
      601 gcgaaccgga gccgttaagg cggtcatcag cgtggaccgg gatggcaacg cggcgatcaa
      661 gggactgatc gccaacggaa cccgtcacga tgagactctc ttctaaggag ttgttgacgt
      721 tcgggccttt cccttcagtc gggagttagg ccgatgccat tttgcccttt tgacgccacg
      781 ccgattgagg cgcgagattc gtcttgatgt gctcagggag cggctcagga cagatagaaa
      841 ccaccatgct tggcatgaag aggtgccaag gctttcgctg gcctagcaac gtcaggcttt
      901 cttgacgaat tccgacttca ggctcatggc accaatgcca tcgattttac aatcgatgtc
      961 gtgatcgctg tcgacgaggc gaatgttttt gaccctggta cccattttga cgaccccgga
     1021 ggagcctttc aatttcaagt ccttgatgac tatgactgta tcgccatccc gtaatgtatt
     1081 tcccgcagag tcacgataaa ccctgccgcc cgcctcatcg tccggagtcg cggcacccgc
     1141 attccactca tgggcgcact cggggcaaac gcaaaggccg ccgtcttcgt aggtaaattc
     1201 gcaattgcat ttggggcatg gtggcagcgt gctcatgacg taatccttac gatacggggc
     1261 gggtcgttat ctcagacctc aaccgctcgc gctccatagc atattcagca aaaatgcgaa
     1321 ccgctttgtg gctggaccaa tccggtcccc cttagccgcc tctgaaagtt tcgatgattg
     1381 cggcagtatc tgttgatccg ccccccacca ttacggccct accgccgcaa tgttttgcaa
     1441 gggtatgttc agtcatgtgc catttgtcag gaagtatgag cggccaacct gtagaagacg
     1501 gcgagcgcgc tcgcaaagag cagggccgca atggcggttg agatgtgaga cccggcgaaa
     1561 tccgagaagg caagcgagcc gctgcgcccg ccgaaggcat cggccgcagc aagcaagacg
     1621 ccggtcatgc tttctcccac caggaatccc gacgcgatga gcacgccccg ccggcgaact
     1681 cttgcctcaa cttcggcttt cccctgggcg tccctgttgg ctgcgcgctt cgcgacagcc
     1741 cgctctgaga accaaccgat cacgcctccg atggcgatcg tcagcgaaac ttcgtaggga
     1801 aggtatatgc cgatgccaac ggtcaaggca ggcagcgaga acccgcgttt gcgccagagc
     1861 cacgactcaa gagccacaag cgcgacgccg agagcagcgc cgataccgat catgttccag
     1921 gcaagctgat gatggatgat gccgttcgca atctgcgtca tgagcgaggc ctggggtgcc
     1981 gccattgcgt tttccgcgag catccccgcg cgcggcaaag acccgacaaa cccgtaattg
     2041 gagtaaagga gctgaagaac cggaacgatt gttatggctc cggcgacaac gccaatggcg
     2101 agcgctacct gctgcttcca aggcgtggcg ccaaccatat ttccagtctt gagatcctga
     2161 agattgtcgt tcgcgatcga ggagacggtt acgacaatcg acgcaatgaa cagcgtcaag
     2221 gccactaccg cgcggctctc ttcaccattg cccatcggaa agacgaagcc gaaaccggcc
     2281 gcgatcagga tcgtgctgag gatgccgatc cccgagatcg ggctgcaaga agagcccagc
     2341 agcgctgcca agtagccgca tgccgatgcc atcagaagac ccagagacat ggtcaggagc
     2401 gtgacagcgg gcaccaccca gtagaggttt gcggcaagat gaacgcctga aacgaaccat
     2461 gcaaacaagc cgcctgtcag aatgcagaga accgcgattg tgccgccgat ccaagcaatc
     2521 gggatgtcgt tctcttcgcg cggcatgttt gcgccatgat gtttatgcgc ctggagcgca
     2581 aggcgaacgc tgtcgaccaa gggcttgatc agagtgagaa cggtccataa accgccgctg
     2641 gcgatgatgc ctgcgccaat gagcctgact ttttcggacc aaaccgcatc cgctgcggcc
     2701 accgggttcc ctgccgaaga cggctctatc gtcatcaaaa ccggcacggc aacgccccag
     2761 gcgataaaga ggccgaggaa caacgccagg caaacgccaa tgcgaacgag gtaacccacc
     2821 ccaatcagag cgagagacaa gctgccgccc atctggaaaa cggccccgcc gtaggagata
     2881 gagcccgaca gcttatcggg aagaaatttg aatccggtcg tcaaaagggt caggccagcc
     2941 gcagcggcgg cgccgaggcc gagctccttc aagccggctt cgctgccagc ttctcctgtc
     3001 ttttcttcgt ccttgtggcc agcgcggagc acctccgccg ccgcaacgcc ttcgggaaac
     3061 ggtagactgc ttcccaccac catcacccgg cgcagcggca cggaataggc gacgccgaga
     3121 agaccgccaa gcagacacac cgccatcgtc tgccagagtg ggaaatcatg ccagaagcca
     3181 atcagaagaa ggcctggaag aacgagaatg acgttgcata acgtacccgc agcggatgcc
     3241 tgggtctgga cgatgttgtt ttccagaatg ccagcaggct tgccgcccga agtcagagcg
     3301 cgcagcacac tcatcgaaat gatcgccgcc gggatggaag acgag
//
LOCUS       DATMGX010001075         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_355627, whole genome shotgun sequence.
ACCESSION   DATMGX010001075 DATMGX010000000
VERSION     DATMGX010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355627"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /locus_tag="VNR65_17660"
     CDS             <1..1699
                     /locus_tag="VNR65_17660"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWJ20548.1"
                     /translation="NADLSNEHFEDEIDFEGFIFPGSVNLSGAVFDRPVSFAGAEFQL
                     PVNFSHAQFHADATFKGAKFSGQAAFDDAVFEGVADFERAEFLKEKNGPLTHSVRFQR
                     ARLMGKADFRSSVMIGSADFSKAQFAGTARFDETRFTADTVFEGAVFSAPAGFHSCQF
                     YGAAAFKETQFTGEARFAEAVFKGECQFEHSQFWNDASFREAHFDSSAHFTDMRVEGV
                     SRFKGAKFAMDANFHDARFTGNTDFSDADFGGASIFCAAQFAQGASWDRCRFLNGADF
                     SGLTLGKASSFKECQFEGDAILREAHFDAPVSFAGARFKANADFSAFQSKVVVVLANA
                     DFKAVPNFLEASFLEPPRLDNMVVADPVKRFHKWKDAGVSDPRRFFKFLRVSSDPDAS
                     VKYRRLKKLAFEAQDLAREQEFFAQEIRCRRFWLDNPLGRGAARFWFGWVYGGVSDFG
                     RSLIRPFLLWLSSIFLFSIYFFALGSGTSWTKCVVGSSDPISEALYLGFRNAFLKVDW
                     NDAVNARRTLGCLYGVEFGGTPVLPPSVASVSLVQAFVSIGFILLFLLALRNLLRLR"
     gene            1772..2164
                     /locus_tag="VNR65_17665"
     CDS             1772..2164
                     /locus_tag="VNR65_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20549.1"
                     /translation="MKIAIATKDWQTVSGHAGKARCWLLYDLQPGKAGAPLPPPAHIE
                     LTHDQLPHYFRDDGPHPLDGVEIVVAGSAGDGFVRHMKNRGADVLLTGETDPSAALTR
                     IIAGEPLPEQRFDITTTLCKLRDLFSQH"
BASE COUNT          392 a          653 c          677 g          523 t
ORIGIN      
        1 gaatgccgat ctttcgaacg agcatttcga ggacgaaatc gacttcgagg gcttcatctt
       61 tccgggctcg gtcaatcttt ccggggcggt tttcgatcgc ccggtttcgt tcgcaggcgc
      121 cgaattccaa cttcccgtca acttcagcca cgcgcagttt cacgcggacg cgacgttcaa
      181 gggggcaaag ttttcaggcc aggcggcttt tgacgacgca gttttcgaag gcgtcgccga
      241 ttttgagcgt gccgagtttc tcaaggagaa aaacgggccg ctgacccaca gtgtgaggtt
      301 ccagcgggcg cgcttaatgg gaaaggccga tttccgctcc tcggtcatga tcgggagcgc
      361 tgatttttcg aaggcgcaat ttgcaggcac ggcgcgcttc gacgagacgc gattcacggc
      421 ggacactgtt ttcgaaggcg cggttttctc ggcgcccgca ggcttccatt cctgccagtt
      481 ctatggcgcc gcggccttca aggagaccca attcacaggc gaggcccgtt tcgccgaagc
      541 ggtcttcaag ggcgagtgcc agttcgagca tagccagttt tggaacgacg cctcgtttcg
      601 cgaagcccac ttcgacagca gcgcgcattt taccgacatg cgggtcgagg gggtgtcacg
      661 cttcaagggc gcaaaattcg ccatggacgc gaatttccat gacgcccggt ttaccgggaa
      721 taccgatttc tcggacgcgg acttcggcgg cgcatcgatt ttctgcgccg cccaatttgc
      781 tcaaggcgcg tcctgggatc gctgccgctt cctgaacgga gccgactttt ccggcctgac
      841 attgggcaag gcctccagct tcaaggaatg ccagttcgaa ggcgatgcca ttctccggga
      901 ggcgcatttc gacgcgccgg tctcctttgc tggcgcgcgc ttcaaggcaa atgccgattt
      961 ctcggccttt caaagcaagg tcgttgtcgt gctcgccaac gcggacttca aggcggtgcc
     1021 aaactttctc gaagcgagct ttctggagcc gccccggctc gacaacatgg ttgtcgccga
     1081 tccggtcaag cgctttcaca agtggaagga cgcgggcgtt tccgatccga ggcgtttctt
     1141 taaattcctg agggtatcct cagacccgga cgcatcggtg aaataccgtc gcctcaagaa
     1201 gctggccttc gaggcgcagg atctggcgcg agagcaggag ttcttcgcgc aggagattcg
     1261 gtgccggcgg ttctggctcg acaatccttt ggggcgaggg gctgcgcgtt tctggttcgg
     1321 ctgggtgtat gggggcgtat cagatttcgg gcgttcgctg atccggccct tcctgctctg
     1381 gctctcctcg attttcctgt tctcgattta tttcttcgct ctcggctccg gcacgtcctg
     1441 gacgaaatgc gttgttggct cctccgatcc catttccgag gcgctctatc ttggcttccg
     1501 aaacgccttc ctgaaggtgg attggaatga cgctgtcaat gcgaggcgga ctctcggctg
     1561 tctttacggc gtagagtttg gcggtacgcc ggttctgcca ccgagcgttg cttccgtgtc
     1621 gctggttcag gcgttcgtga gtatcggctt tatcctgctg ttcctgctcg cgctccgtaa
     1681 tttgctcagg cttcgatagc gcccttcggc tagtgccgca acgcttgagc tgtgtgttat
     1741 ggtatatagc gtgccggaat gagggaaggt catgaagatc gcaatcgcta ccaaggattg
     1801 gcaaaccgtg agcgggcacg cggggaaggc ccgatgctgg ctgctttacg atctgcagcc
     1861 cgggaaggcc ggcgcgccat tgccgccccc ggcgcacatc gagcttacgc atgatcagtt
     1921 gccgcattac tttcgcgacg atggtcctca tccgcttgac ggcgtggaaa tcgtggtggc
     1981 aggcagcgca ggcgacggtt tcgtgcggca catgaaaaat cgaggcgccg acgtgctgtt
     2041 gaccggcgaa accgatcctt cggcggcgct gacacgcatc attgccggag agcctctgcc
     2101 cgagcagcgc ttcgacatca ccacaacatt gtgcaagctg cgcgatctgt tctcgcagca
     2161 ttgatctcct cgttcttgta gcagaacccg cccatctgat atcgccgtca gaacgttcga
     2221 ctcgcatcag tggctcatga acgag
//
LOCUS       DATMGX010001076         2105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_321252, whole genome shotgun sequence.
ACCESSION   DATMGX010001076 DATMGX010000000
VERSION     DATMGX010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321252"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(588..1019)
                     /locus_tag="VNR65_17670"
     CDS             complement(588..1019)
                     /locus_tag="VNR65_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20550.1"
                     /translation="MSLYNTFRIVVGIVLSGAIGWVSLFAFNAVKIWQQLEAFEASGP
                     REGNIHMIPAAMESAINTMQGGSAVFLAAGIVGVLLGEVFRTRSLVFYVGATGALTAV
                     FAAAFWHEGGPLGTAPAAAALAMTGFVAGGIYWMIASPSQG"
     gene            complement(1036..>2105)
                     /gene="rlmN"
                     /locus_tag="VNR65_17675"
     CDS             complement(1036..>2105)
                     /gene="rlmN"
                     /locus_tag="VNR65_17675"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWJ20551.1"
                     /translation="ANQVRMRVTQLWNWIYARGAQDFADLTNIAKALRADLDARFTLA
                     RPEIAAEQISEDGTRKWLLRLQPQRPGERAPEIETVYIPEPDRGTLCISSQVGCTLTC
                     TFCHTGTQRLVRNLTPAEIIGQILVARDRIGDWPGATAPADGRGIPEAERKITNIVLM
                     GMGEPLYNFDNVKDAIAVASDPDALSFSKRRITLSTSGVVPMIERAGAEMGVMLAISL
                     HAVRDELRDELVPINRKYPIAELLAACRAYPSLSNARRITFEYVMLKGVNDSVAEAKE
                     IVRLLAGIPAKINLIPFNPWPGTRYECSDWGQIERFADIVNRAGYASPIRTPRGRDIM
                     AACGQLKSASEKLKASALRET"
BASE COUNT          475 a          612 c          601 g          417 t
ORIGIN      
        1 ccgcaaatga aagttgtggg gaaagtcggc gcgaacgaaa cgatactgct ggcgcgtgac
       61 taaaggcagg agcagccatt tgatctagct caatcgaaag attgccagcc gctctttcaa
      121 ggatgtttcg ccaggactcc gacataagaa ctcacccttc tgctacgcat gactacggct
      181 ttgtaataaa atattgtggc aacgctgaga aaaaaattag acagtgatta aaaatcaaca
      241 aaaacactgg ctttattttt tgtctagaga aaagatcttt gctatattgg aaaaaaccat
      301 agactcttcg ctggaagcgg ccgcgaggta taacaacgtt cgaacgcagc aggttattcc
      361 gaggctggct ctgagaacgc gccttgatat tctagcttcg cgttgatgtg cttgattgac
      421 ttatggttag cgcgatgaat gccggatcac ccgcgcgcct cgggaagcca ataccctcac
      481 ttttgaaata gttcttgttt tctctaatga agcttaagct tgaaggcaaa atgcggaaga
      541 aacgaggcga tcccatcgat gcatcgcgag agcgcacagc gagcgcttca tccctgcgac
      601 gggctggcga tcatccagta aatgccaccg gcgacaaaac cagtcatggc gagcgcggca
      661 gcggccggag cagtgcccaa cgggccgcct tcgtgccaga acgccgctgc aaacactgcc
      721 gttagcgcgc ccgttgcgcc gacgtagaac accagagagc gcgttctgaa gacctctccg
      781 agcagcactc ccacgatgcc agccgcgaga aataccgcgg acccgccctg catggtgttg
      841 atcgcgcttt ccatggcggc cgggatcata tggatattgc cctcgcgggg accggacgcc
      901 tcgaaagctt cgagctgctg ccagatcttc acagcgttaa aggcaaaaag agagacccag
      961 ccgatcgcgc cggacaggac aattccaacg acaatacgga aagtattata aagactcatc
     1021 gtctccctcg gcctctcacg tctcgcgaag tgcgctcgct ttcagcttct cgctcgcaga
     1081 cttcagctgc ccgcaggccg ccatgatgtc ccggccgcgc ggtgtcctga tcgggcttgc
     1141 atagcctgcc cgattaacga tgtcagcaaa gcgttcgatt tgcccccaat cagagcattc
     1201 ataccgcgtg cccggccagg gattgaacgg gatcagattg atcttcgcag gaatgccggc
     1261 aagcagtcgg acaatctctt tggcctcggc gacggagtca ttcacgccct tcagcatcac
     1321 atattcaaaa gtgatccggc gcgcgttgga caggcttgga taggcgcgac acgccgcgag
     1381 tagctccgcg atcgggtatt tgcggttgat cggcacaagc tcatcgcgca attcatcacg
     1441 aacggcatgc agcgagatgg cgagcatcac gcccatttcc gcgcccgcac gctcgatcat
     1501 cggaacgacg ccggatgtgg aaagcgtgat gcgacgcttg gagaaggaca aggcgtcggg
     1561 atcggaagcg acggcgatgg cgtccttcac gttgtcgaaa ttataaagag gctcgcccat
     1621 gcccatcagc acgatattgg tgatcttgcg ttcggcctcc gggatgccgc gcccatcggc
     1681 cggagcagtc gcgccgggcc aatcgccgat gcggtcgcgc gccacgagga tctgcccgat
     1741 gatttccgca ggcgtgagat tgcgcacgag ccgctgggtg ccggtatggc aaaaggtgca
     1801 tgtcagagtg cagcccacct ggctcgagat gcagagcgtt ccgcgatccg gttcggggat
     1861 gtagacggtc tcgatctccg gcgcacgctc gcctggcctt tgcggctgca gccgcagaag
     1921 ccatttgcgg gtgccatcct ccgaaatctg ctcggccgcg atctccggcc ttgccagcgt
     1981 gaagcgcgca tcaagatcgg cgcgcaaagc cttcgcgata ttggtcaagt ccgcgaagtc
     2041 ctgggcgccg cgcgcgtaaa tccagttcca gagctgggtc acacgcatcc tgacctggtt
     2101 cgccg
//
LOCUS       DATMGX010001077         4324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_209194, whole genome shotgun sequence.
ACCESSION   DATMGX010001077 DATMGX010000000
VERSION     DATMGX010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209194"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2987)
                     /locus_tag="VNR65_17680"
     CDS             complement(<1..2987)
                     /locus_tag="VNR65_17680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWJ20552.1"
                     /translation="MLNRLVEFALTQRAFILILSVVIAGLGTYALLNIPIDAFPNIAS
                     VQAKIIMKAPGMTPEEVETRVITPLELELQGIPRQTILRSSAKYAIADITIDFEDGTD
                     IYWARQQVAERIAGIRDELPANVTGGLAPISTPLSDVFMFTIESDTLSLAERRTLLDW
                     TIRPALRTIPGVADVNALGGFVRVFEVVPDSAALAAAGLGVSDLSGVIAANNRNDGAG
                     RLNEGEKALVVRAEGAIKTPQDIEQIVLKSENGRILRVSDVAAVRIGSLTRYGAVTKN
                     GSGETVEGLVLSLRGANASAVVKAVRARLDELQPRLPPGVSIHVFYDRADLIQRAVQT
                     VTKALIEATVLVVILLLLFLGNLRAALVVAVTLPMAALITFSMMQLFGMTANLMSLGG
                     LAIAIGLIVDGAVVVVENTEEWLSREEEAGIPFLHRLYHAASEVALPVASGIVIICLV
                     FLPLLSLQGLEGKLFAPVAITIILALSGSLLLSLTLIPVLASLVLRKGHGNPLLMRVL
                     LPFYSGLLRTCLRRPWPVYATAVSGAALAIVAYLAVGKAFMPSMDEGSAIMQTTKLAS
                     IGLRRSIETDSLIQRTLLEKVPEIKEIIARTGADELGLDPMGLNETDVFLVLKPKEEW
                     RVQDKEWLLKQMRDVMADLPGREIAFTQPIEMRTSEMLTGSRGDLAVKIFGPDLKVLA
                     DLAGQMKAVLTKVPGAAEVMTVSNATVDYLEVKIDRMMAGRTRLSVGRIEDELRALLE
                     GTPAGIVSEEGRRTNIIIRGTEQARDAPEQFALTQLAAGDGGLVRVSDVAQLTRTGGP
                     VKVDRENASRFAIIQAYVAGRDLVSFVEDAQKAVAQKVNLPNGYKLVWGGEFENQRRA
                     AARLTIVVPMALGLIFVVLFATLRSIRQSLLILANIPFALVGGILALWVSGEYLSVPA
                     SVGFIALLGIAVLNGLVLVSHFNELLAGGMPLETVVFEGARRRLRPVLMTASIAAFGL
                     VPLLSATGPGSEIQKPLAIVVIGG"
     gene            complement(2990..>4324)
                     /locus_tag="VNR65_17685"
     CDS             complement(2990..>4324)
                     /locus_tag="VNR65_17685"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWJ20553.1"
                     /translation="NCKRSFTMSSKQTDEVLVWDPFISGSHWLLAPAILVDWFTHKPR
                     WMHAWNGYLEATLVVQRVYVSFRFPKSITETKGGIEIRPTLPTSRSSIVRHLGLRLSL
                     SSFLFLALCVSAFAQTIAINDRQLDALQIRLEKVRTAATEAVVLLPATVVPPLNSRVA
                     VSAPFAGTVLEVDVLVGQHVKEGTPLVTIASREMLEAVSRLRQSEMELQAAEVIANRH
                     KQLADRHVGLLTKAEETQTEVNKLRVATEQHRRIASLGNLKVNGDGSYTLSAPRAGQV
                     VETRVLPGSSLDTMAAAVIIDTSDELWIQAQLPAIFINRIAEGDGILIANSVVGRVIS
                     VGSSLDPVTRSASLLARLPKGSGLVQGQMVAATVARPAPNGTLTVPSPAITWVEGKTV
                     VFLRTNEGFAVAPVSLRSKTADVATIDGGKIKEGQQVAVTGVVQLEKMMAGE"
BASE COUNT          939 a         1327 c         1226 g          832 t
ORIGIN      
        1 ccgccaatca cgacgatggc aagcggcttt tgaatttcag atccgggccc tgtcgcggac
       61 aggagcggca ccagaccgaa agccgcgatg cttgccgtca tcaagacggg acgcagcctt
      121 cgccgcgcac cttcgaacac cacggtctca aggggcatgc cccctgcaag cagttcgttg
      181 aagtggctga ccaagacaag cccgttcaac accgcgatgc cgagcaaggc gataaaacca
      241 acagaagcgg gcaccgaaag atattcgccc gaaacccaca acgcgaggat tccgcccacg
      301 agagcgaagg ggatgtttgc gaggatgagc aaagactggc gaattgacct aagggtcgcg
      361 aacagcacca cgaatatcag cccaagcgcc atcggcacaa cgatggtgag acgggcagcc
      421 gctcgccttt ggttctcgaa ctcaccgccc caaaccaatt tgtacccgtt cgggagattg
      481 accttctgcg ccacggcctt ctgcgcgtct tccacaaagc tcaccagatc acggcccgca
      541 acataggcct ggataatcgc gaaccgcgac gcgttctccc tgtccacttt gaccggtccg
      601 cctgttctcg tcagctgcgc cacgtcagag acccggacca agcctccgtc tccagccgct
      661 aactgcgtca gcgcaaactg ttcgggcgca tcgcgcgcct gctccgtgcc tcgaatgata
      721 atgttcgtcc gccggccctc ctcggacacg attcccgctg gcgtgccctc aagcaaggca
      781 cgtaactcat cctcgatgcg cccgacggaa aggcgggtcc gccccgccat catgcggtca
      841 atcttcactt cgaggtaatc aactgtcgca ttcgaaaccg tcatgacctc ggcggcgccc
      901 ggcaccttgg tgaggactgc cttcatctgt cccgccagat ccgccagcac cttgaggtcc
      961 ggcccaaaga ttttgacggc gagatccccc cgcgagcctg tcagcatctc tgacgtacgc
     1021 atctcgatag gctgggtaaa cgctatttcc cggcctggaa gatccgccat cacgtcccgc
     1081 atctgtttga ggagccattc cttgtcctgc acgcgccact cttccttcgg cttcagcaca
     1141 aggaaaacgt ctgtctcgtt caatcccatc ggatcaagac caagctcatc agcgccggtg
     1201 cgagcgatga tctccttgat ttcgggcacc ttctcaagga gcgtgcgctg aataagggag
     1261 tcggtttcga tgctgcggcg cagcccgatc gaggcgagct tcgtcgtctg catgattgcc
     1321 gagccttcgt ccatcgaagg catgaacgct ttgccgaccg ccaaataagc aacgatagca
     1381 agggctgccc cgctcacagc agtcgcgtaa actggccagg ggcgtcttaa acaggtccga
     1441 agcaggccgc tgtagaaggg cagcagcaca cgcatgagta agggattgcc atgccccttc
     1501 ctgagcacga gcgatgcaag caccggtatc aacgtcaagg aaagaagaag cgatccgctc
     1561 aaggccaaga tgatcgtgat agcgacgggc gcaaagagtt tgccttccaa gccttgaagc
     1621 gaaagcagcg gcaggaaaac gaggcagatg atgacgatgc ctgaggcgac aggcaacgca
     1681 acttcggatg ccgcatgata aaggcgatgc agaaagggaa tgcccgcctc ctcctcacgc
     1741 gaaagccatt cctccgtatt ctcgaccaca acgacggcac cgtccacgat gaggccgata
     1801 gcgattgcga gcccgcccag gctcatcaag tttgccgtca tgccaaacag ctgcatcatc
     1861 gaaaatgtga tgagcgccgc catcggcagg gtcacggcta ccacgagggc ggcgcgcaaa
     1921 ttccccagaa atagcaggag aagaataacg accagaacag ttgcctcgat aagcgccttc
     1981 gttacggttt ggacggcacg ctgaatgaga tcggcgcggt cgtaaaagac atgaatggaa
     2041 acgccggggg gcaatcttgg ctgcaactcg tcgaggcgtg cgcgcacggc tttcaccacg
     2101 gcactcgcgt tggccccgcg cagcgaaagc acaaggcctt cgacagtctc gccgctgccg
     2161 ttcttcgtga cggccccgta tcttgtcaga cttccaatgc ggacggcggc cacgtcgctc
     2221 acgcgcagaa tgcggccgtt ctcagatttc agaacaattt gctcgatatc ctggggtgtc
     2281 ttgattgcgc cctcagcgcg aacgacgagc gccttctccc cttcgttcag tctgcctgcg
     2341 ccgtcgttgc ggttatttgc tgcgattacg ccgctgaggt cggagactcc gaggcccgcg
     2401 gccgccaatg cggcgctgtc ggggacaact tcgaagacgc ggacaaatcc cccaagcgcg
     2461 ttaacatctg cgacgccggg aatcgtgcgc agcgcgggcc tgatggtcca atccaagagc
     2521 gtccggcgct cggcgagaga gagcgtatcg ctttcgatcg tgaacatgaa gacatcggag
     2581 agcggcgttg atattggagc aagtccgcct gtcacgtttg ccggaagctc atcgcggatg
     2641 cctgcaatgc gttcggccac ctgctgcctc gcccaataga tatcggtgcc atcctcgaag
     2701 tcgatggtga tgtcggcgat tgcgtatttg gcggacgaac gcaggatcgt ttggcgcggg
     2761 atcccctgca actccagctc gagaggcgta ataacccggg tttccacctc ctcgggcgtc
     2821 atgcccggag ccttcatgat gatcttcgcc tgcaccgagg cgatattggg gaacgcatcg
     2881 atgggaatat tcagaagggc gtaagtcccc agcccggcga tcacgacgga taaaatgagg
     2941 atgaacgcac gttgggtgag ggcaaactcg acaagcctgt tcagcatcgt cactctccag
     3001 ccatcatctt ttcaagctgg acaacgccgg tgacggcaac ctgctggcct tccttgatct
     3061 tccccccgtc gattgtggcc acgtccgcgg tcttgctgcg cagcgaaacc ggcgcaaccg
     3121 caaacccttc gttcgtgcgg aggaatacaa cggtcttgcc ttcgacccag gtgatcgccg
     3181 gggacggcac ggtaagggtc ccattaggcg ctggccgcgc aaccgtggcc gccaccatct
     3241 gaccctggac aagaccggat ccttttggca accgcgcgag aagggacgcc gaccgcgtta
     3301 ccggatcgag ggaagatccg acagaaataa cacggcccac gaccgagttt gcgatcagaa
     3361 tgccgtcgcc ttcggcgatg cggttgataa agatcgcggg cagttgagcc tgtatccaga
     3421 gttcgtcgct ggtgtcgatg atgaccgccg cggccatcgt gtccaggctg cttccgggca
     3481 agacgcgcgt ttcgactacc tggccggctc tcggtgcgct taaggtgtaa cttccgtctc
     3541 cattgacctt caggttaccg agcgatgcga tgcgtctgtg ttgctcggtg gcgactcgta
     3601 atttattgac ctcggtctgt gtttcttccg cttttgtcaa caacccgaca tggcggtcgg
     3661 caagctgctt gtggcgattt gcaatcacct ccgcggcctg tagctccatc tcggattgcc
     3721 tgagccgcga cacagcttca agcatttcgc ggctggcaat cgtaacaaga ggtgttccct
     3781 cctttacgtg ctgtcccaca aggacatcga cctccagaac cgtgcctgcg aacggcgccg
     3841 agaccgccac gcggctgtta agaggcggaa cgacagtcgc gggtaagagg accacggcct
     3901 ctgtcgcggc cgtccgcact ttttccagcc ggatttgcag cgcgtcaagc tggcggtcat
     3961 tgattgctat tgtttgtgcg aaggctgaga cgcacagggc aagaaataag aaggaagaaa
     4021 ggctaagccg taatcccaaa tgccgcacga tagaggatcg actggtcggc agcgttggac
     4081 ggatttcgat cccgcccttt gtttcggtta tcgatttcgg aaaacgaaac gacacgtaca
     4141 cacgctggac aaccagcgtc gcctcaagat agccgttcca agcatgcatc cagcgcggct
     4201 tgtgcgtaaa ccagtcgacc agaatagctg gcgccagcag ccaatgagag ccgctaatga
     4261 acgggtccca aacaagtact tcgtcggtct gtttagaact catcgtaaat gaccttttac
     4321 aatt
//
LOCUS       DATMGX010001078         2253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_187061, whole genome shotgun sequence.
ACCESSION   DATMGX010001078 DATMGX010000000
VERSION     DATMGX010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187061"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1528)
                     /locus_tag="VNR65_17690"
     CDS             complement(179..1528)
                     /locus_tag="VNR65_17690"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HWJ20554.1"
                     /translation="MSESLPPPSDPPTEVVAREMRGRAKEMFEHALSECSIPRAFSKH
                     VLYDRGELRLGNDVYALGSFSRTLVVSIGKAGHTMAQALANIIGTGLTGIVACPNAPP
                     AQLFGFRYFMGGHPAPNEESLRSGDAILRLLSGLSPRTLAVYLISGGASAIAEKPISA
                     GISLADVVETYKALVHSGAPIAEINAIRKHLSALKGGRLARVATPSYQASVLVSDVPE
                     GSLDALASGPTMPDTTTLADCYAIVNRYRLLKNFPASVRTLFEREQLEETPKPNDPVF
                     ERSRYTTVLSNATAVNAAVERAVLAGFAVEVDNRCDDWDYERAADHLLGRLRELRRGL
                     SRACLISGGEVTVKVGARSGVGGRNQQFALYCAQKIAGEHITVLSAGTDGIDGNSNAA
                     GAVVDGTTVERAQQRGLDIPTALAHFNAYPLFDAIGDSIMTGPTGNNVRDLRILMAY"
     gene            1864..>2253
                     /locus_tag="VNR65_17695"
     CDS             1864..>2253
                     /locus_tag="VNR65_17695"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20555.1"
                     /translation="MPGTGTTDYWQLATGYCYHLAVMPVATDVSSSTYVPVKAPRGNQ
                     LSCKGWGQEAAMRMLMNNLDPDVAERPQDLVVYGGTGRAARSWECYHAIVKSLKALEN
                     DETLLVQSGKPVGIFQTHEYAPRVLICN"
BASE COUNT          458 a          683 c          671 g          441 t
ORIGIN      
        1 atacttcaat gccgaatggc cctgccgtcg cctttaccac atgatgttga actcggaccc
       61 agggatcgac ctggtgaccg atacgatcct ggcaaccatg gaagcgctgg aacggcaagc
      121 gcaaccggta gggtaatcag atcgacattt ccgacgaacc tggcgacagg caaccgcttc
      181 aatatgccat caaaattcgg aggtcccgca cattgttgcc ggtcggccct gtcatgatgc
      241 tgtcgccgat ggcgtcgaac agagggtagg cattgaaatg cgccaaggcc gtggggatat
      301 ccaatccgcg ctgctgggcc cgttcgaccg tggttccatc caccaccgcg ccggccgcat
      361 tgctgttgcc atcgatgccg tcggtccccg cgctcagcac agtgatgtgc tcgccggcaa
      421 tcttttgtgc acaataaagc gcgaactgct ggttgcgtcc gccaacgccc gacctcgccc
      481 ctacctttac cgtaacctcc ccacccgaga tgaggcacgc gcgtgataag cctcggcgta
      541 gctcgcgcag ccgacccagc agatgatcgg cggcgcgttc gtagtcccag tcgtcgcagc
      601 ggttatccac ctcgacggcg aagccggcca acaccgcgcg ttcgaccgca gcgttgaccg
      661 cggtggcgtt cgaaagcacc gtggtgtagc gggaacgttc aaacacagga tcgtttggct
      721 tgggcgtttc ctccaactgc tcgcgttcga acagcgtgcg cacactggca ggaaagttct
      781 tcagcagcct gtaacgattc acgatggcgt aacagtcggc gagagtggtg gtgtctggca
      841 ttgtgggacc cgaggccagc gcatcgagag atccctccgg tacatcggag accagtaccg
      901 acgcctgata ggaaggcgtg gccacgcgcg ccagccgtcc gcccttcaac gccgaaagat
      961 gtttgcggat ggcattgatc tcggcaatgg gtgccccaga atgcaccagc gccttatacg
     1021 tctcgacaac atcggcgagc gaaatgcccg cgctgatggg cttctcggcg atcgcagaag
     1081 ctcctccgct gatcagatag acggccagcg tgcgcggcga aaggccactc agcagccgca
     1141 ggatggcatc gccggagcgc agcgactcct cattaggggc cggatgtcct cccatgaaat
     1201 agcgaaagcc aaacagttgc gctggcggcg cgttgggaca ggcaacgatt cccgtgagcc
     1261 cggttccgat gatgttggcg agcgcctgcg ccatggtatg tcccgccttg ccgatcgaga
     1321 cgacaagcgt tcgcgagaac gaccccagcg catagacatc gtttcccagg cgtagctcgc
     1381 cgcgatcgta tagcacgtgt ttcgaaaatg cccgcggaat actgcattcg gaaagagcgt
     1441 gctcgaacat ctctttcgcg cggccgcgca tttcgcgggc tactacttcc gtcgggggat
     1501 cggagggtgg cggcaaggac tcggacatgc ggggagatta taagctggag tctggcaggt
     1561 tcgcccttgc cgccagctat tctctcttgc ttggaagtga ttcgatccgc agtccaatta
     1621 ctccgagtta gcttcagcac gactgtaaaa gggcgtccac ttggactgcc cgcagcatgg
     1681 cggattaatc aagaattcat gttctgttta tcgttctctg atgtactcgc gcggcagggc
     1741 gggtaagcta ccttcaattg caaacctgat cggagagaat ggggagatgg ccaaacagcc
     1801 gctccccgat tttattgcag tagccggtag ccagttgtcg gtagccagta ggcggttttc
     1861 agagtgccgg gaactgggac aactgactac tggcaactgg ctactggcta ctgctaccat
     1921 cttgctgtca tgccagtcgc taccgatgta tccagttcta cctatgtccc cgtcaaagcg
     1981 ccgcgcggca accagctctc ctgcaaaggt tgggggcagg aagccgccat gcgcatgctg
     2041 atgaacaatc tcgaccccga cgttgccgag cgcccgcagg acctcgtggt ctatggcggc
     2101 accggacgcg ctgcgcgcag ttgggagtgc taccacgcca tcgtcaagtc gctgaaggcg
     2161 ctcgaaaatg acgagacgct gctggtgcag tcgggcaaac cggtcggcat ctttcagacg
     2221 cacgagtacg cgccccgcgt gctcatctgc aac
//
LOCUS       DATMGX010001079         2751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_244434, whole genome shotgun sequence.
ACCESSION   DATMGX010001079 DATMGX010000000
VERSION     DATMGX010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244434"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1096
                     /locus_tag="VNR65_17700"
     CDS             260..1096
                     /locus_tag="VNR65_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20556.1"
                     /translation="MVGLLPTGDLAWLMPHHKGLSEDVLHNFALTPPNSPRPCLAYRT
                     VAGAGTAASNEPFNVGKFREVEIDDCLKRFGSGAAAQTVGQRGEPPGIFALKCEQLGN
                     RVAPTLGTAASVGWLLRRWLRQAGGVASLVAGPDQRIGVASRVRGLGRAFCRHGHDLR
                     CVTHGRFRRQDLPADSRRVTVHSPKRLFPSHAPCSTPLGAQVARSSRAGEHSEHPPFA
                     PACPPWHLSWNGMAQRCYSGGRCGGAARGSQEDRDSLTSSPPSPVELAGSFRGVVKEP
                     RR"
     gene            2052..2288
                     /locus_tag="VNR65_17705"
     CDS             2052..2288
                     /locus_tag="VNR65_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20557.1"
                     /translation="MGQEESWRARPQGGWGAQVGTLHLVVLQGEKFAHFLISKFPNAV
                     CQSGTRADVGAAKAAAHTEAHRLERLFAAGRQVA"
     gene            2403..>2751
                     /locus_tag="VNR65_17710"
     CDS             2403..>2751
                     /locus_tag="VNR65_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20558.1"
                     /translation="MIDIAAIKARFEALEPVLDERERRLFAASEARVAGRGGVVAVSR
                     ATGIARSTIDRGLADLRSGAVMFSTRVRRRGGGGKPATETQPGLLEALNDLVHRFHET
                     DQLCRIPRAAPDIV"
BASE COUNT          529 a          808 c          915 g          499 t
ORIGIN      
        1 ccagtggcga tttcgcaaca ggctccccac ccttccttat gctccctatc cgcccccctg
       61 ttagaccgtt ccggctgggc cgttgcctcc cgggcagtag ggggacgccg ccgcaatcca
      121 tgccaacggg agtaaacgtg ccccaaacca tcccccagga cacgcttagt gccgcggctg
      181 gccatccacg tcttctacta cagcaccagg tatggtgccg tagcccgaca agtcggatgg
      241 cgggacagcg ataaggtaga tggtcggttt gctgcctacc ggagacttgg cgtggctgat
      301 gccgcaccac aaggggctct ccgaagatgt gttgcacaac ttcgcgctga cgccaccgaa
      361 cagccctcgc ccgtgtctgg cgtaccgcac tgtcgctggc gcaggaacag ccgcgagcaa
      421 cgagccgttc aatgtcggca agttccgtga ggtcgagatc gacgattgtc tcaagcgctt
      481 cggcagtggg gctgccgctc agaccgtcgg gcagcgcggc gagccacctg gtatattcgc
      541 cttgaagtgc gagcaactcg gcaaccgcgt cgcgccaacg ctggggacgg ctgcgtcggt
      601 cggatggctg ctccggcggt ggctacgtca ggccggcggg gtagcaagct tggtcgccgg
      661 gcctgaccag cgaatcggcg ttgcctcccg ggtgcgcggc ctcgggcgcg cgttctgtcg
      721 gcatggtcat gatctccgtt gtgtgacgca tggtcgcttc cgccgccagg acctgcccgc
      781 cgattcgcgg cgggtcacag tgcattctcc aaagcgcctg tttccaagcc atgctccttg
      841 ttcgacgccg ctcggcgcgc aggtcgcccg ctcttcgcgg gcgggcgagc atagtgagca
      901 cccaccattt gccccggctt gcccgccgtg gcatctatcc tggaacggta tggcgcaacg
      961 atgctactca ggcggccggt gcggcggcgc cgcaagagga tcgcaagagg atagagattc
     1021 ccttacgagt agccctccga gtcctgtaga gctggcgggg tcgttccgcg gtgttgtaaa
     1081 ggagccgcgc cgttgagctg agatcaaaac cccactcgac ttcatctgta agcttcgctg
     1141 ccagcaggaa ttgcttagcg gttgtggaga gcgggatctc aaacgtggcg aaatctgtga
     1201 tccgggtccg ctgccacgac tgaggaggaa gacttcgagg ctgcgacggc ctccgctagt
     1261 ttgctagagg tcgaaatgaa gcgggcgagc agagggagcg aagggctgga tcgcgcgctg
     1321 tgcgcgcgat ccagtttggc gcgcggcgcc tctccgaaat caaatgagct agcggcacag
     1381 gagtcaggca aaccagccct ggcgtcgcac agcgaatcct tgcgcgcagc agcccgccga
     1441 ttaccgctcc gccgtccgcc agttgctcat aacgacgcat ctccacactc ggcgagagtc
     1501 ttgggcagtt ctgccgcggc caagcggggc tgccgcagag agtcggacat ttacctggca
     1561 tcctgaaccg tgttgcgatc atggcgcttg atcgccgccg tggggaatta ccggagctcc
     1621 atcgcactat cgacctgccg cgcgacaggt cggggctctt cgtcttggcc aaacaaatat
     1681 tatcacgttc gctcgaccag atgcaatggc gcgcggccat gtggctgaat acatgcagat
     1741 gaacaaaatg gggtcagagc gccactggct gaagtctcac gtcagcaggt tgtggtggtg
     1801 catgtggcgg tggcataact caagaatcat tagatagctc cgacgacgtt cgctagaaga
     1861 tcggccaccg ccggttctca tcctttggac agcgaatagg ggagccgcaa gagtcccacg
     1921 acggcgcgcc cgtctcgccg gaaggcacgg gcgctgctgt tggctgaaag aattgaagag
     1981 tcactgctcc aggtaacgga gtggtgcgct aaaggtgctg aaggcactca aaatagcaag
     2041 ggaggaagac gatgggacaa gaagagagct ggcgagcacg cccgcaaggc ggctggggtg
     2101 cgcaggttgg caccctgcat ctcgttgttc tgcaaggcga gaagtttgct catttcctga
     2161 tatccaagtt tccaaacgcg gtgtgtcaat ccgggacgcg ggccgacgtc ggtgccgcga
     2221 aggcagctgc ccacacggaa gcgcacaggc tggagcggct gtttgcggcc gggcgccaag
     2281 tagcttaggg cccgagccag ggcaaccgaa tcggaagaaa agaatcggga agcggataaa
     2341 aacgcaacga aggaattcgg gaattggcgt gatgcggccg ctggagcgtt tatcctgatt
     2401 gggtgatcga catagctgcc atcaaggctc gcttcgaggc gcttgagccg gttttggacg
     2461 agagagagcg gcggttgttc gcggccagtg aggcgcgcgt ggccggacgg ggcggggtag
     2521 tcgcggtatc gcgggcgacg gggattgcgc gcagcacgat cgatcgcggc ttggcggacc
     2581 tacgttcggg cgccgttatg ttcagcacgc gagttcggcg acgcggtggc ggcggcaagc
     2641 cggcgacgga gacacagcca gggcttctgg aagccttgaa tgacctagta caccgattcc
     2701 atgaaaccga ccagttgtgc cggattccca gggcggcacc agatatagta g
//
LOCUS       DATMGX010001080         4699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_213770, whole genome shotgun sequence.
ACCESSION   DATMGX010001080 DATMGX010000000
VERSION     DATMGX010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213770"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="VNR65_17715"
     CDS             complement(<1..1550)
                     /locus_tag="VNR65_17715"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWJ20559.1"
                     /translation="MDATKQASVHAIVSLEAAPGHALRVLKKPRGIDLGRLLVTLATF
                     LVLLLAAASVAPALMDWKAYRPDIEQAASALLGRKISIIGDIDIALLPEPHFHAKKVV
                     AEGGPSEAAQMSAEAVDLTLSLQGLLSGRVEASKLKLVSPFLILDLSKPLQSASAPAK
                     TGPLSIAAEVTSLEIEGGRISVYPDAGQPEALILTGVDGALAAPQHGNAYRFTGRVSQ
                     NSQRYDVKFNAAAQGSGVKLTGSATELRSKAVIQADGVLNTANAPVFDGTLAAMAPQT
                     LVGASFDIQAKAGAKVSRAGLALTDLVLTLDPGSRPQVLTGSANVAFTPSTVDITLQA
                     RALDADVLVSAASGPGAPGTAAGWDTFRTAAEQLLWLYPNFGVHLSLASDQIQLRGEL
                     IEGAKAEGTRTPQKWLFEDAQATLPGETTIRLTGALKKTAAKPELMARVALEGKNLGR
                     LNRWIAPASTNARSGPARVFTAGALFTLSDDVTAFTDVKGDVDGTPFTANLRLDKTPA
                     RKLKVALAG"
     gene            complement(1798..2508)
                     /locus_tag="VNR65_17720"
     CDS             complement(1798..2508)
                     /locus_tag="VNR65_17720"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HWJ20560.1"
                     /translation="MTNQATQQMLSAGAAMLGFGHKKVETTHGPFTLPPLPYATSKNE
                     PAIDAQTMQLHHDKHHAAYVNALNQAAGSGGSWTRKPLQDILASLSAVPGSVRTAVRN
                     NGGGHANHTMFWQIMGGRGGTPAGDVASAIDRDLGGFSAMQNTFNDAGAKIFGSGWVF
                     VTVDKAGKLALTTRPNQDTPLMDGERVLFGNDVWEHAYYLKYQNRRADYLKAWWSVVD
                     WPKIGERYAEAKAGTLTS"
     gene            complement(2609..3121)
                     /locus_tag="VNR65_17725"
     CDS             complement(2609..3121)
                     /locus_tag="VNR65_17725"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HWJ20561.1"
                     /translation="MTKLAILASSVLALFAANAACAHEYHVKDLTIFHPWARPAAEGQ
                     TGAVYLTIKNDGKEGDKFLSAESPAATKVELHETSEENGVMKMRAVKDGAEVKPGSSL
                     AFKPGGYHLMLFDLKKPLEEGAMVPLTITLAKAGAINVDIKVEKTAPGTAEAMPMHTH
                     SMKGMDHSGH"
     gene            3353..3793
                     /locus_tag="VNR65_17730"
     CDS             3353..3793
                     /locus_tag="VNR65_17730"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HWJ20562.1"
                     /translation="MKQHIGFTAALGLSILIGNAALAETAQSHWPAGAKVFFVEPKNG
                     AEISGPVKVVMGVKGLDIAPAGTEKPKTGHHHILIDTDAPTGEKALAPLPAEDNIKHF
                     GKGQTETALTLAPGKHTLQLVVGDGNHIPYDPALASEKIIITVK"
     gene            complement(3970..>4699)
                     /gene="proC"
                     /locus_tag="VNR65_17735"
     CDS             complement(3970..>4699)
                     /gene="proC"
                     /locus_tag="VNR65_17735"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWJ20563.1"
                     /translation="AGFPPASLAVREPQPSPDLAALLMANGIADQPPSAADVLVLAVK
                     PQIMAKVLDEVAPDAGAQTAVLSIAAGRTVASIARHFSPDTAIVRAMPNLPAEIGRGI
                     TAAFANPHVTTAQRDLCGKLLRAAGDVAWVEDESYLDAVTAVSGSGPGYVFYLVECLA
                     AAARDAGLPPDLAEKLARATIEGAGELLRRSPLSPAQLRENVTSPGGTTAAGLSVLMR
                     EQGLTDLMRETVAAAAKRSRELSE"
BASE COUNT          915 a         1433 c         1381 g          970 t
ORIGIN      
        1 cccgcaagcg caaccttcag cttgcgagca ggcgtcttat cgaggcgcaa gtttgccgta
       61 aacggggtgc cgtccacgtc gccttttacg tctgtaaagg ccgtgacgtc gtccgaaagt
      121 gtgaacaatg cgcctgctgt gaaaactctc gcggggccgc ttctggcatt tgtcgaagcg
      181 ggtgcgatcc agcggttgag ccggcctaag tttttgcctt cgagagcgac acgagccatc
      241 aattccggtt tcgcggcggt cttcttgagc gcgcctgtga gcctgatggt agtttcgccc
      301 ggcagggtcg cctgggcatc ctcgaatagc cacttctgag gcgttcgcgt gccctcggct
      361 tttgcacctt ctatgagttc gcctcgaagt tggatttgat cgctcgccag cgaaagatgg
      421 actccgaagt tgggatagag ccaaagcagc tgctctgcgg cggtccggaa ggtgtcccag
      481 cccgcagccg tgcccggcgc ccccggtcca gacgccgctg acacgagcac gtcggcgtcc
      541 agtgcgcgcg cttgcagcgt gatgtccaca gtactcggcg taaaagccac gtttgccgat
      601 cctgtgagaa cttgcggccg gcttccgggg tccagcgtca ggacaaggtc ggtcagggct
      661 agacctgcgc gactgacttt cgcccctgcc ttcgcctgga tgtcgaaaga ggctcccaca
      721 agcgtctgag gcgccatcgc cgccagcgtt ccgtcaaaaa cgggagcatt ggccgtatta
      781 agtacaccat cggcttggat cacggccttc gaccgaagct ctgtcgcact tcccgtgagc
      841 ttcacgccgc tgccctgcgc cgctgcattg aattttacgt cgtagcgctg gctgttttga
      901 gaaacccgcc ctgtgaagcg gtaagcattc ccgtgctgcg gggcagcgag tgctccatca
      961 acgccggtca agatcaacgc ttcgggttgg ccagcatccg gataaacgga aatccggccc
     1021 ccctcgattt cgaggctcgt tacctctgcc gcgattgaga gcggcccggt cttggccgga
     1081 gcagacgcgc tttgcagcgg cttcgacaga tcaaggatga gaaaagggct gacgagcttg
     1141 agcttgctcg cctcgacgcg gccgctcaac aagccctgaa gggacaaagt gagatcgacc
     1201 gcttctgcgc tcatctgagc ggcctcggac gggccgcctt cagccacgac ctttttcgcg
     1261 tgaaagtgcg gctcgggcag aagcgcgatg tcgatatcgc cgataatgct gatcttgcga
     1321 ccgagaagag cggaggcggc ctgctcgatg tctggcctgt aggctttcca gtccatcaat
     1381 gctggcgcga cagaggccgc ggccagcaga agaaccagaa acgtcgcaag tgtaaccagc
     1441 aggcggccca aatcaatccc tcttggcttc ttcagaaccc gcagcgcatg gcccggcgct
     1501 gcctctagcg atactattgc atgcactgaa gcctgttttg tggcgtccat cgcgggcgcg
     1561 aaagcaggcc tcttatgctt gcccgattct cgaatgtaac cgcccatgtt cctctgtaaa
     1621 agttacgagc ggtccacaat tccttcttca cttcgcaaag gagtgtttgc cgaatgcgac
     1681 agcgcatcgc cgccaccgtc atcccggaaa ggcgcagccc aatccgggat cgtaacaaat
     1741 tgaagcgcct ctctttttgt gatcccggct tgcgcttcgc ttggccgtca tgacgaatca
     1801 ggaggtcagc gttccagcct tggcctcagc ataacgctcg cctattttgg gccaatcgac
     1861 cacgctccac caggctttca gatagtcggc gcggcgattc tgatatttca gatagtaggc
     1921 atgctcccac acatcattgc cgaaaagcac gcgctcgccg tccatcagcg gcgtatcctg
     1981 attgggccgc gtagtgagcg cgagcttgcc agccttgtcc accgtgacga agacccaacc
     2041 ggagccgaaa atcttcgcgc ccgcgtcgtt gaaggtattt tgcatagccg aaaagccacc
     2101 gagatcgcgg tcgatggcgg atgcaacatc gcctgcaggc gtgccgccgc ggcctcccat
     2161 gatctgccag aacatggtgt gattggcgtg gccgccgcca ttgttgcgga cggcggtgcg
     2221 aacgcttcct ggcaccgcgc taagactggc caggatgtcc tgcaaaggtt tcctcgtcca
     2281 gctgccgccg ctgcccgccg cttggttgag ggcattcacg taggctgcat gatgcttgtc
     2341 gtgatgcaac tgcatcgttt gcgcatcgat tgcaggctcg ttcttgctgg tggcgtaagg
     2401 caggggcggg agggtgaacg gcccatgcgt ggtttccact ttcttgtgac cgaagccgag
     2461 cattgccgcg ccggcagaga gcatctgttg cgtcgcctga tttgtcattg ggttctcctc
     2521 atattgtttc caataagaga gagaaccagc gagccgccgc attggacgca acgcggcttt
     2581 ctgccagagc gacgtgccga tcaaattatc aatgcccaga gtgatccatg cccttcatgc
     2641 tgtgcgtgtg catcggcata gcctccgcag tgccaggcgc ggttttctcc actttgatat
     2701 ccacgttgat ggctcccgcc ttcgccagcg tgatcgtcag cggcaccatc gctccctctt
     2761 caagcggttt cttgagatcg aagagcatga gatgataacc gccgggcttg aacgcgagcg
     2821 aggagccggg cttcacttcg gccccatcct tgacggctcg cattttcatc acgccgttct
     2881 cctcgctcgt ttcgtgcagt tcgactttcg tggctgccgg gctttccgcg gaaagaaact
     2941 tatcgccctc tttgccatcg ttcttgattg tgagatagac ggcgccagtc tggccctcag
     3001 cagccggacg cgcccaaggg tgaaagatcg tcagatcttt gacgtgatac tcgtgagcgc
     3061 acgcggcatt cgcagcgaaa agcgcaagca cgctggaggc tagaattgcg agttttgtca
     3121 tggtgacccc cttgtctctt gcgggacgct aacccgctac ccgatgatgc gccctaattt
     3181 ggggaggttt ctcttttccc gttacccttc ttatcgggtc gaatgcggga tctgacctca
     3241 tcgcgaaaag ttcaacacgg aaaattcttt cgccgttgaa aaagccttcc gtgaagctgc
     3301 ttttccctgt agttccggcc acattcgccg tgtcggcaaa agggaggatt tcatgaaaca
     3361 gcatattggg ttcaccgcag ctttggggct ttcgatcttg atcgggaacg cggcattggc
     3421 ggagaccgct caatcgcact ggcctgcagg cgcgaaggtc tttttcgtcg agccgaagaa
     3481 cggagcggag atttcaggcc cggttaaggt cgtgatgggc gtgaaagggc tcgatatcgc
     3541 accagcgggt accgaaaagc cgaagaccgg gcatcatcac atcttgatcg acacagacgc
     3601 accaaccggg gaaaaggcgc tcgcgccatt gcctgcggaa gataacatca agcatttcgg
     3661 gaagggccag accgaaacgg cgctgacgct tgcgccgggc aagcacacgc tccaactcgt
     3721 cgtcggcgac ggaaatcaca tcccctacga tccggcgctc gcctcggaga aaatcatcat
     3781 caccgtgaag tagcgcatca agcttgtcag gcgcgccagc ctgagagggt ttacaattgg
     3841 ctaaaattgg tttcgtttcg taaaaccgcc ctaagtaagc atataagcac ttctctcgcc
     3901 ccgtcaacgg atggccattg gcaaagcgta gaggcttagg gcggtccgag atcaaacgag
     3961 acccgagtgt tattccgaca gctcgcggga acgcttcgcc gcagcggcga cagtttcccg
     4021 catgaggtcc gtgaggccct gctcacgcat aaggacggaa agcccggcgg cggtcgtgcc
     4081 gcccggggac gtcacattct cacgaagctg cgcgggcgaa agcggcgacc tgcgcaaaag
     4141 ctcgcctgcg ccctcgatgg tggccctggc taatttctca gcaagatcag gcggcaaccc
     4201 cgcatcgcgg gccgccgcag cgaggcattc gaccagatag aagacatatc ctgggccgga
     4261 tcccgaaaca gcggtcaccg catcgaggta gctctcatcc tcaacccaag cgacgtcgcc
     4321 cgccgctctg agcagcttgc cacaaagatc gcgctgtgcg gttgtgacat gcgggttcgc
     4381 gaacgcggcc gtgatccccc gcccgatctc ggccggaaga ttgggcatgg cgcgcacgat
     4441 cgcggtgtct ggcgaaaagt gccgtgcaat actggccacc gtgcgtccgg ccgcgatgga
     4501 gagcacggcc gtttgcgctc ccgcatcggg cgcaacctca tccagcacct tcgccatgat
     4561 ttgcggcttc acggccagga cgagaacatc cgccgcggag gggggctgat ccgcaatccc
     4621 attcgccatc agcagcgccg ccaagtccgg tgacggttgc ggctccctca cagctaacga
     4681 ggccggggga aatcccgct
//
LOCUS       DATMGX010001081         2682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_473549, whole genome shotgun sequence.
ACCESSION   DATMGX010001081 DATMGX010000000
VERSION     DATMGX010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473549"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VNR65_17740"
     CDS             <1..637
                     /locus_tag="VNR65_17740"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWJ20564.1"
                     /translation="PAQSAQMTPPPQGSQQPNPSQQMAQMQSATPQANPKLPMPDQGS
                     GKQIDFAQMMKTPGDLTAQAARSVAEHRGSGGSYGGGGGDYGAGPGGRARTLGALDVL
                     SDTQGVDFGPYLSRVVDAVRRNWYTIIPEAARAPLLKRGKVAIEFAILPDGRVAGMKI
                     TGVSGDVSLDRAAWGGITASNPFAPLPSEYHAPYLALRFRFYYNPQKGDIE"
     gene            complement(664..921)
                     /locus_tag="VNR65_17745"
     CDS             complement(664..921)
                     /locus_tag="VNR65_17745"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWJ20565.1"
                     /translation="MALKVQLAGTAFKYLNSLDKPTRTRIKDKLEAIAKAPEDPRLSY
                     PLSASTKRSTRIGPYRVLFEIDEETLFVAHIAPRGQVYRKA"
     gene            complement(922..1434)
                     /locus_tag="VNR65_17750"
     CDS             complement(922..1434)
                     /locus_tag="VNR65_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20566.1"
                     /translation="MATASEVLNNFGIDNWNAKVYGSLSTAAQLSLLVKNRAQALRMS
                     YLLWKLNSQTAKFFQEVDDVMTGKHVAAQTQPCEPISLERLEQSRDSLIQLGINLGRI
                     YEDARRNRLTNHSLIAGPVTALRAHADHFLELAEWFDSFQRMDEIEQAFASAREEKRR
                     GEVYDLSQVR"
     gene            1630..1815
                     /locus_tag="VNR65_17755"
     CDS             1630..1815
                     /locus_tag="VNR65_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20567.1"
                     /translation="MNPGQQPGYKPDSDAREHDADWKARYRSRERAKVFGLLVLMIFI
                     LALAFFRFGRTIPWGAR"
     gene            1816..>2682
                     /gene="miaA"
                     /locus_tag="VNR65_17760"
     CDS             1816..>2682
                     /gene="miaA"
                     /locus_tag="VNR65_17760"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWJ20568.1"
                     /translation="MSTPPPIQSEPLLVVILGPTGSGKSALAIALAQQFAGEVVSCDS
                     VAIYRHFEVGTAKPTSQQRALAPHHLLDVAEPDQPFTAGEYARQARIVIEEIANRQRL
                     PIVVGGTGLYLRALLDGLFPGPQRSEGLRERLRERAQERGSPYLHKLLLRLDPAAAAK
                     IHPNDVPKIIRAVEVCLASRAPMSQLWEQRGRDPLRGFRILRIGLNPEREALYARLNQ
                     RACQMFDNGLIDETKSLLERYGERKSVTPLESLGYKQAAQFLREELTREQALAATQQG
                     HRNYAKRQMTWFR"
BASE COUNT          536 a          778 c          795 g          573 t
ORIGIN      
        1 tcccgcacag tcggcgcaga tgacgccacc gccgcaaggc agccagcaac cgaacccgtc
       61 gcaacagatg gcacagatgc agtcggccac gccgcaggcg aatcccaagc tgcccatgcc
      121 agaccaaggc agcggcaagc agattgactt cgcgcagatg atgaagacgc cgggcgacct
      181 cacggcgcag gccgctcgtt cggttgccga gcatcgcggt agcggcggct cttacggcgg
      241 tggtggcggc gattacgggg cgggtccggg tggaagagcg aggacgctag gggccctgga
      301 tgtattgagc gacacccagg gcgttgactt cgggccttat ctctctcgcg ttgtcgatgc
      361 cgtgcgcaga aactggtaca ccatcatccc cgaagcggcg cgtgccccac tgctcaaacg
      421 cggcaaggtt gccatcgagt tcgcgatcct gccggatggc agggttgcag gcatgaagat
      481 cactggagtg tcgggcgacg tgtcgctgga tcgcgccgcc tggggtggaa tcaccgcctc
      541 gaacccgttc gctcccttgc catcggaata tcacgctccc tatctggcat tgcgtttccg
      601 cttctactac aacccgcaaa agggcgatat cgaatagctc ccacgctgtg gtgcagtttg
      661 aattcaggct ttacggtaaa cctgtcccct gggagcgatg tgagcgacga agagagtttc
      721 ctcgtcgatt tcgaatagaa ctctgtaagg gccaatgcgg gtgcttctct tggtgctggc
      781 cgagagaggg taagagaggc gagggtcttc aggcgccttc gcaattgcct ccaatttgtc
      841 cttaattctg gttcttgtgg gcttgtcgag agagttcaga tacttgaacg ctgtgcccgc
      901 gagttggact ttcagcgcca atcaccgaac ctgcgataag tcgtacacct cgcctcggcg
      961 cttttcctcg cgcgcgctgg caaatgcttg ctcgatctcg tccatgcgtt ggaacgaatc
     1021 gaaccactct gccaattcaa ggaaatgatc ggcgtgtgcc cgaagagcgg ttacgggtcc
     1081 ggcaatcaac gaatgattgg ttaatctgtt ccgtctcgca tcctcgtaaa tcctgcctag
     1141 gttgattccg agctggatca aggaatctct cgattgttca agcctttcga gtgaaatagg
     1201 ttcacagggc tgcgtttgcg cggcgacgtg tttccccgtc ataacgtcgt ccacttcctg
     1261 aaagaatttt gcggtttgcg agttcagctt ccagagcagg tatgacatcc ttaacgcctg
     1321 ggcgcgattc ttcactaaga gcgagagctg cgcagccgtc gagaggctgc cgtacacttt
     1381 agcgttccaa ttatcgattc caaagttgtt gaggacttcg cttgcggtag ccactggctt
     1441 ctctcccgcc ccagaggaag ggtccggacg cgcacacata taccacggca catctaagta
     1501 gacgcaccag cctatattag gggtgcgcta ggatcaagga aagtttacca gctttgtccc
     1561 gttagaggcg ttcaaggccg ctagaattca aactgcacca gtgccaactc tcacgcgtag
     1621 aatggggttg tgaaccccgg ccaacagccc ggatacaagc ctgactccga tgctcgcgag
     1681 catgatgcag attggaaggc gcgatatcgt tcgcgtgagc gcgcgaaagt tttcggcctt
     1741 cttgtcctca tgatcttcat ccttgccctg gcgttcttcc gtttcggccg gaccatcccc
     1801 tggggtgcgc gttgaatgag cactccgcct ccgatccaat ctgaaccgct ccttgtcgtt
     1861 attcttggac cgacaggttc gggcaaatcg gctctcgcga tcgctcttgc gcaacagttc
     1921 gccggcgaag tggtcagttg cgattcggtg gccatctatc gtcacttcga ggttggaacg
     1981 gccaagccaa catcgcaaca acgggcgctg gcgccgcatc atcttctcga tgtggctgaa
     2041 cccgaccaac ccttcaccgc aggcgagtat gcgcggcagg cgagaatagt cattgaggaa
     2101 attgccaacc gccagcggct tcccatcgtc gttggtggta cgggtcttta tctgcgagcg
     2161 ctgttggacg ggctcttccc agggccgcaa cgttcggaag gtttacgcga gcgccttagg
     2221 gaacgcgcgc aggagcgcgg ctcgccttat ctgcacaagc ttctcctgcg tctggatccg
     2281 gcggcggcag cgaagatcca tcctaacgac gttcccaaga ttattcgcgc tgtggaagtg
     2341 tgtctggctt cgcgcgcgcc gatgagccag ttgtgggagc aacgcgggcg cgatccgctg
     2401 cgcggatttc gcattctgcg gatcgggctt aatccggagc gggaggcgct gtatgcgcgg
     2461 ctcaatcaga gggcctgcca aatgttcgac aacggcttga tcgacgaaac aaaatctctg
     2521 ctcgagcgat acggcgagcg gaagagcgtc acgccactag agtcgctcgg ctacaagcag
     2581 gcggcgcaat tcctgcgcga agaacttacg cgcgagcagg cactggcggc gacgcagcaa
     2641 ggccatcgca actatgccaa gcggcagatg acctggtttc gc
//
LOCUS       DATMGX010001082         2605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_243431, whole genome shotgun sequence.
ACCESSION   DATMGX010001082 DATMGX010000000
VERSION     DATMGX010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243431"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VNR65_17765"
     CDS             complement(<1..305)
                     /locus_tag="VNR65_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWJ20569.1"
                     /translation="MTDLLWLSDDQLDCIKPYFPLSHGVPRVDDHRVVSGIVFVIKNG
                     LRWRDAPREYGPHKILYNRFIRWSRMGIFDRIFANLAGRSGQPDRLIIDATHLKAHRT
                     "
     gene            complement(508..1959)
                     /locus_tag="VNR65_17770"
     CDS             complement(508..1959)
                     /locus_tag="VNR65_17770"
                     /inference="COORDINATES: protein motif:HMM:NF005365.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWJ20570.1"
                     /translation="MMNDGISLEMLAFNLKLLGRKSRKKVLISAGKAENKKRMLPAIQ
                     QFLELEVDIYSTPGTHNFLAIYGIESIEIHKITDRRSPNILTFLNENRFDLVINVLTG
                     DNDYDESSDANLIRKLCIENGIPLITDCDVGIATLQQIFRDTSKGTFRYKLSDNSEPW
                     NLRLHFMEAVEKLGGIANHHAHFDKAYLINMENLRLSQVDMQKKWEIYRYLKENYTHD
                     DLVERISRGLERMIEQGVTYCRTMVDADSTVGLLPIMAAVEVKKRYADKITFEVGVQP
                     LQGVLDRASYEQYAEACAMADYCGGLPSRDRPRPEAHLDIVLDLAKRLGKPVEVHVDQ
                     ENNPLENETELLAAKTIEHGMQGRVFGIHAISVGAKEEREQDRIIAKILEADMGIVIC
                     PSAALSMSKLQMNAPLHNSIAPFPKLHQAGVRCYLGIDNIADLFMPMVDGDIWTECRM
                     LMEACRFYDINTVAKWACAKPIPSQAQPAALAA"
     gene            complement(2126..2443)
                     /locus_tag="VNR65_17775"
     CDS             complement(2126..2443)
                     /locus_tag="VNR65_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20571.1"
                     /translation="MLRPISLLMLSALAACATAGSTPPERQFVLADTGKYKTEAEKQR
                     AALIAQNDCKVKAMTASAAIEKSITSERNGMENLSRAREKGAEMYTSSFTLCMLNAGF
                     TQR"
BASE COUNT          563 a          696 c          727 g          619 t
ORIGIN      
        1 gttcggtgag ctttgagatg agtggcgtcg ataatgagac ggtctggctg tcccgatcgg
       61 cctgccaggt tcgcaaaaat tcggtcgaag atgcccatac ggctccagcg aataaatcgg
      121 ttatacaaga tcttatgggg accatactcc ctcggcgcgt cccgccagcg aagcccgttc
      181 ttgatcacaa aaacgatccc gctcacaacg cgatgatcgt caacgcgcgg cacaccgtgc
      241 gagagcggaa agtacggctt gatgcaatca agttggtcgt ccgacagcca aagtaaatcg
      301 gtcatggcag gtcctcctgc caaacccttg aatctgattt ggaacccgcc gggaatctta
      361 aaattaatag gtcctgagcc tagtattgat atgcataata ctttctaatc tatccccata
      421 aaacgacggg aggcgttcgt caccaaaaac agacgcccct tccgtccaac ggagccggtt
      481 accgcgccaa ggaagtgcgg gtagaactta cgcggcgagc gcggcgggct gcgcctgcga
      541 cgggatgggt ttcgcacaag cccatttcgc gacggtgttg atgtcgtaga agcggcaggc
      601 ttccatcagc atcctgcatt cggtccagat gtcgccgtcc accatcggca tgaagagatc
      661 ggcgatgttg tcaatgccga gatagcagcg cacacccgcc tgatgaagct tcgggaatgg
      721 cgcgatggag ttgtgcagcg gcgcgttcat ttgcagtttg gacatgctaa gcgcggccga
      781 tgggcagatc acgatgccca tgtcggcttc gaggattttc gcgatgatgc ggtcctgctc
      841 gcgttcttcc ttcgcgccga ctgaaatcgc gtggatgccg aagacgcggc cctgcatgcc
      901 atgctcgatg gtcttcgcgg cgagcaattc cgtctcgttt tcgagcgggt tgttctcctg
      961 atccacatgc acctcgaccg gctttcccag ccgcttggca aggtcgagga caatgtcgag
     1021 atgcgcttcc gggcggggac ggtcgcgaga cggaaggccg ccacaataat cggccatcgc
     1081 gcaggcttcg gcatattgct cgtaggaggc acgatcgagg acgccttgca ggggctgcac
     1141 gccgacctcg aaagtgatct tatcggcata gcgcttcttg acttccaccg ccgccatgat
     1201 cggcaacaga ccgactgtac tgtccgcgtc gaccatcgtt cggcaatagg tcacgccctg
     1261 ctcgatcatt ctctcgaggc cgcggcttat ccgctcgacc aggtcgtcgt gggtatagtt
     1321 ttctttgaga tagcggtaga tctcccactt cttctgcatg tcgacctggc tgaggcgcag
     1381 gttctccatg ttgatgaggt aggccttgtc gaaatgggcg tggtggtttg cgatgccgcc
     1441 cagcttttct accgcctcca tgaagtggag gcgcaggttc cacggctcgg agttgtcgga
     1501 gagcttgtaa cggaacgtgc ctttgctggt atcgcgaaag atctgttgca gcgtggcgat
     1561 acctacatcg cagtcggtga tgagcggaat gccgttttcg atgcagagct tgcgaataag
     1621 attggcgtcc gacgactcgt catagtcgtt gtcgcccgtc aacacgttga tgacgaggtc
     1681 gaaacgattt tcattcagga aggtcagaat attgggagaa cggcgatctg taattttatg
     1741 aatttcaatg ctttcgatgc cgtatatggc aagaaaatta tgtgtacctg gtgtggaata
     1801 aatatcgact tcaagttcta gaaattgttg tatcgcaggc aacatgcgct ttttgttctc
     1861 ggccttgcct gcgctgataa gaaccttttt ccggctcttt ctgcccaaca gcttcaaatt
     1921 gaaggcgagc atctccagcg aaattccatc gttcatcatt gaccccttgc gttctccccc
     1981 ctttactcgt gcgcgcacag cgccaactcg cggacccacc ctttccacaa attgccgcat
     2041 cctgatgtgt acgaagcgcg tagtcaagtt gcaaaatcgc gcggctggat gacaaatggt
     2101 taagccggcg cagcagaaaa catatctatc tctgtgtgaa gccggcgttg agcatgcata
     2161 gcgtgaagga actcgtgtac atctcagccc ccttctcacg ggcgcgagac aaattctcca
     2221 tgccgttgcg ctcggaagtg attgatttct caatcgccgc gctcgccgtc atcgctttga
     2281 ctttgcagtc attttgcgcg atcagcgccg cgcgttgttt ttctgcttcg gttttatatt
     2341 tgcccgtatc ggcgaggacg aactgcctct cgggcggggt tgagcctgcg gtggcacatg
     2401 cggcgagcgc tgaaagcatc aggagggaaa tcggacgcaa cacgaaaatt ttatcctgaa
     2461 ttgaaaaact gatacctcaa atagcacatt gcaggcggca tgagatatcg aagttcaagc
     2521 aaatcaggac acccgtgtcc cagcgttgac gtgggatgcg gcgataacat gctgatataa
     2581 ctttaggaaa aggatttgtt ggctg
//
LOCUS       DATMGX010001083         2461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_326770, whole genome shotgun sequence.
ACCESSION   DATMGX010001083 DATMGX010000000
VERSION     DATMGX010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326770"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /gene="infC"
                     /locus_tag="VNR65_17780"
     CDS             <1..631
                     /gene="infC"
                     /locus_tag="VNR65_17780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWJ20572.1"
                     /translation="LLARICASSLARRALFLQSIAFVHNLGELPIRRPNRAPPAATKD
                     GPRTNDEIRNAQVQLIDQTGLNHGTVETVAAVKMALEAGMDLVEISPNTNPPVCKIMD
                     YGKFKYSAQKKAAEARKKQKVVEIKEIKLRPMIDDHDYDVKMRAMQRFFEEGDKVKIT
                     LRYRGREMAHQEIGTKLLDKVKADVAEYAKVEQDARFEGRQVVMVLAPR"
     gene            complement(750..2198)
                     /locus_tag="VNR65_17785"
     CDS             complement(750..2198)
                     /locus_tag="VNR65_17785"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWJ20573.1"
                     /translation="MEHPTETSELIELFQQQLADVRQQLTEKDAIIAALREEVAELKR
                     RLELNSSNSGKPPSSDGLKKPPRVMSLREPSGKKTGGQKGHPGETLRRVENPDVIVNH
                     YPEACSKCDVKVAPDMSTGHRSRQVVDIPEPKVVVTEHRAHGCACPNCDTVTWASFPA
                     EVKAPVQYGARICAFVVYLLNYHFLPEDRLAELMSDLFGLKLVPATIARMSAACAERF
                     GDFADAVREHVAAARVKHLDETGFRIGGRTQWLHIFSTALLTFYRVSSKRGSLLSGVT
                     GIVVHDHWKPYYTMKGVLHALCNAHHLRELKALVEIEKEEWARKMQRLLRRACHAVNL
                     ARERDVSLKLHVIELFQRSYDRILKEGLAFHEGQPAFIRAPTEGQRKRRGRQRRRTGH
                     NLLLRLSTRNEDVLRFLTDPDVPFTNNQAEQDGRMMKVKQKISGGFRSEDGANTFVVN
                     RTVISTAKKQAWNVLQTLTQDSRTLILSLRLA"
BASE COUNT          498 a          732 c          712 g          519 t
ORIGIN      
        1 gcttttggcg cgcatatgtg cgagttcgct tgcccgccga gccttattcc ttcaaagtat
       61 cgccttcgtt cacaaccttg gagagctacc cattcgccgt cccaacagag ccccgcccgc
      121 tgccaccaaa gacgggccgc gcaccaacga tgaaatccgc aacgcacagg ttcagctgat
      181 cgaccagacc ggcctaaacc acggcacggt cgagacggtg gctgccgtca agatggccct
      241 cgaggccggc atggaccttg tcgagatttc gcccaatacc aatcctcccg tttgcaagat
      301 catggactac gggaagttca agtattcggc gcagaagaag gccgcggaag cccgcaagaa
      361 acagaaggtc gtcgagatca aggagatcaa gctgcggccg atgatcgacg atcacgacta
      421 cgacgtgaag atgcgcgcga tgcagcggtt cttcgaggag ggcgacaagg tcaagatcac
      481 cctgcgctac cgtggccgcg aaatggcgca tcaggaaatc ggcaccaagc tgctcgacaa
      541 ggtcaaggcc gacgtggccg aatacgccaa ggtcgagcag gacgcccgtt tcgaaggccg
      601 tcaggtcgtc atggtgctgg cgccgcgctg attgaaatca gccattcgcg tttgtgaata
      661 ttgcgcgttt gtgaatatcg aatacggccc gccaggcgat ctggcgggct tttttctttg
      721 cgtgtgactg cccaggtacc gggtcatggt caagccaatc gaagggatag aatgagggtc
      781 cttgaatcct gagtgagggt ctggaggacg ttccaagctt gctttttggc ggttgagatg
      841 acggtacggt tgacgacaaa ggtattggcg ccgtcttccg atcggaagcc gccggagatc
      901 ttctgcttga ccttcatcat gcgcccatcc tgttcggctt ggttattggt gaatggaacg
      961 tctggatcgg tcaagaagcg tagcacgtct tcgttgcggg tactcaggcg cagcaagaga
     1021 ttgtgaccgg tgcgccgccg ctgacggccg cggcgcttgc gttgcccttc tgtgggcgcg
     1081 cggatgaacg cgggctggcc ctcatgaaag gcgagtccct ctttcaggat gcggtcgtaa
     1141 cttcgctgaa atagttcgat gacatgcagt ttgagtgata catctcgctc tcgggcaaga
     1201 ttgactgcat gacaggcgcg gcgcagcagt cgctgcatct tgcgcgccca ttcctccttc
     1261 tcgatctcca caagagcttt cagttcgcgc agatggtggg cattgcacag ggcgtgcagg
     1321 acgcccttca tcgtataata cggcttccag tgatcatgca cgacgatgcc ggtaacgccg
     1381 gacaacaggc tgcctcgctt ggatgaaacg cgatagaagg tcagcaacgc ggtcgagaaa
     1441 atgtgcagcc attgcgtccg cccaccgatc cgaaacccag tctcgtccaa gtgttttacc
     1501 cgcgccgccg ccacgtgctc ccgcacggca tcggcgaagt cgccaaagcg ctcggcgcag
     1561 gccgcactca tccgggcgat ggtggccgga accaatttca ggccgaacag atcagacatc
     1621 agctctgcaa gccgatcctc aggcagaaag tggtaattta gcagatagac cacaaaggcg
     1681 cagatcctcg cgccatattg gacaggtgct ttgacttccg cgggaaatga cgcccacgtg
     1741 acggtgtcgc agtttgggca cgcgcacccg tgggcgcgat gttcggtcac gacgaccttc
     1801 ggttcgggta tatcgacgac ctgccgggat cggtgcccgg tactcatgtc cggcgccacc
     1861 ttcacgtcgc acttcgagca ggcctcagga taatggttaa cgatgacgtc cggattctcg
     1921 acccgacgca gggtctctcc cggatgacct ttctgtccgc ctgttttctt gccggatggt
     1981 tcccgcaagc tcatgacccg cggcggcttc ttcagcccgt cgcttgatgg cggcttgccg
     2041 ctgttgctgc tgttaagttc caaccgccgc ttgagctcgg ccacctcttc tcgcaacgcc
     2101 gcaataatcg cgtccttttc ggtcaactgt tgccgaacat ccgccaactg ctgttgaaac
     2161 agttcaatca gctcggaagt ttcggtggga tgttccatcc cacccttgag tccgattcac
     2221 cgctgtcgcg ccagctcctc aacccgtctg agtcaattcg ccgcagccaa agtccgccga
     2281 ccaacccgcc tgttcagctg gggtgcgcca ccgcctacct agggagtcac gatttcaaaa
     2341 catttttagg gccacctgcc aaagcgggta gggttctcga gacaaatcga cgatttccgc
     2401 ctttggcgtg tgccgtgaca aaggcggtgc tttacagggg tttatcccca atcgcgcgat
     2461 g
//
LOCUS       DATMGX010001084         2330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_440151, whole genome shotgun sequence.
ACCESSION   DATMGX010001084 DATMGX010000000
VERSION     DATMGX010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440151"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /gene="lptC"
                     /locus_tag="VNR65_17790"
     CDS             <1..672
                     /gene="lptC"
                     /locus_tag="VNR65_17790"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HWJ20574.1"
                     /translation="RKARRHSLLVRVLKVLLPTMAAAIVSLYLLPSLFKVSIDKGRGT
                     ASVRTVTLDAGTLKMLDPHVKGVNERNEPYDFSADSATQASKNAEEMFLENVRGHMTG
                     QDGKITTLTAPNGVHNNKADQLTFNNGVVVKREPDLAATFQTATAFMKQQTVISKTPV
                     IVRLHESTIHAETMTMFWGEQRTIFEGNVRTHLERKADEAAPQRLEAQPSPAGGWAAE
                     SAPAR"
     gene            692..1447
                     /locus_tag="VNR65_17795"
     CDS             692..1447
                     /locus_tag="VNR65_17795"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HWJ20575.1"
                     /translation="MALCRTGGKVRRQGTVVMLVALALLALPGGAPAQTPGSGFSSLG
                     GSNSKKPIDIESDRLEVDDQKHVAIFSGNVSATQGDHNLRAPRLEVTYDKTAEAAPQD
                     QGGAKAAKPIRPANTNANSSADPLSSGQIKFIHASGGKVVVTSKVDDQEATGDDVIYD
                     VKGQKITMTGKEVVLTQKKSVVKGRQIDIDLATGRATVIPEHGRVRAILTQDDAKGSM
                     STNPLTGARKKDTDSAAEAPKSSSGVNGPMQNH"
     gene            1475..2311
                     /gene="lptB"
                     /locus_tag="VNR65_17800"
     CDS             1475..2311
                     /gene="lptB"
                     /locus_tag="VNR65_17800"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HWJ20576.1"
                     /translation="MVLLDKHHRGPLPQRPPQRARIQPIPDPHADPRRRHAVEERLVV
                     DRVVKTFKKRTVVKGVTINLKRGESVGLLGPNGAGKTTVFYMITGLIKPDEGSVFIDG
                     YDVTKLSMYRRARLGIGYLPQEASIFRGLSVEDNIMTVLQVTEPNKKERKRRLDELLE
                     EFKIERLRKSPSLALSGGERRRCEIARALASRPAYMLLDEPFAGIDPLAINDIRTLVR
                     HLTDRGIGVLITDHNVRETLDLIDRAYIIYDGRVLIEGTPAEVVANEEVKRVYLGDMF
                     SR"
BASE COUNT          534 a          658 c          694 g          444 t
ORIGIN      
        1 cgtaaagcca ggcgccacag cctgctcgtc cgggtactca aggtgcttct tcccacaatg
       61 gcggcggcga tcgtaagcct ctatctgcta ccctcgcttt tcaaagtctc gattgacaaa
      121 ggccggggca cggcatcggt gcgcacggtc accttagatg cggggacgct gaaaatgctg
      181 gacccgcatg tcaaaggcgt caacgaaagg aatgagccct acgatttttc cgcagattcg
      241 gccacccagg cgtcgaagaa cgcggaggag atgtttcttg aaaatgttcg cggccatatg
      301 accggacagg atggcaagat cacaacgctg actgcgccaa atggcgtcca caacaataaa
      361 gcggatcagc tgacattcaa caatggcgtc gtcgtcaagc gtgagcctga tttggctgcc
      421 acgtttcaaa ctgcgaccgc attcatgaag cagcagaccg tgatttcgaa aacgcccgtt
      481 atcgttcgcc ttcatgagag cacgattcat gcggagacta tgacgatgtt ctggggcgag
      541 caacggacga ttttcgaggg aaatgtcagg acgcatttgg agcggaaggc cgatgaggcg
      601 gctccccagc ggttggaagc gcagccttct ccggctggag gttgggccgc agaatcggcc
      661 ccggcgcgat aaatcgcgga gcacttttgc aatggcgctg tgtcggacag gtgggaaggt
      721 gaggcggcaa gggacggtgg tcatgctggt ggcccttgcg cttttggcgc tacccggagg
      781 agctccggct cagacccctg ggtcaggatt cagcagcctc ggcggctcaa attccaaaaa
      841 gcccatcgat atcgagtccg accggctcga agtcgacgat cagaaacacg tggcgatatt
      901 cagcggaaat gtttcggcca ctcaagggga tcacaatttg cgcgcgccgc gtctggaggt
      961 gacctacgat aagacggcgg aggccgcccc tcaggaccaa ggtggcgcaa aggccgcgaa
     1021 gcccataagg cctgcaaaca cgaacgccaa ttcctccgca gacccgctat cgagtggtca
     1081 gatcaaattc attcacgcgt cgggaggcaa ggttgtcgtc acaagcaagg ttgacgatca
     1141 ggaagcgacc ggcgacgatg ttatttacga cgtgaagggt caaaaaatca ccatgacggg
     1201 gaaggaagtt gtcctcaccc aaaagaaaag tgtggtgaag gggaggcaga tcgacatcga
     1261 tcttgccacc ggccgcgcaa ctgttattcc ggagcatgga cgagttcggg ctatccttac
     1321 gcaggatgac gccaaaggct caatgtctac caatccgttg accggcgcca ggaaaaagga
     1381 cacggattcg gctgcggagg cgcctaagtc atcctcgggg gtcaacgggc cgatgcagaa
     1441 tcactaagtt tttttagcgg gcggcaaaaa gtccatggtc cttctcgata aacatcaccg
     1501 tggccctttg ccgcaacggc ctccgcaacg cgcgcgcatt cagcccattc cggacccgca
     1561 tgcagacccc cgcagaaggc acgcggtgga agagcggctc gtcgtcgata gggttgtgaa
     1621 gaccttcaag aagcgcaccg tcgtcaaagg tgtcaccatc aacttgaagc ggggcgagtc
     1681 cgtcggcctt cttggcccga acggcgccgg caagacgacc gtattttaca tgattacggg
     1741 ccttatcaag ccggacgagg gctccgtctt catcgacggc tacgatgtca ccaagctttc
     1801 catgtaccgg cgcgcgcggc ttggaattgg ctatcttccg caggaagcgt caatttttcg
     1861 cggcctgtcg gtcgaggaca acatcatgac cgtcctccag gtgacagagc cgaacaagaa
     1921 ggagcgcaaa cgccgcctcg acgagcttct ggaagagttc aagattgaac gcctgcgcaa
     1981 atcgccctcc cttgctctgt ccggcggcga gcgtcggcgc tgcgagatag cgcgcgcgct
     2041 ggcgagccgc cccgcctaca tgcttctcga cgaacctttc gcgggaattg atcctcttgc
     2101 catcaacgat attagaactt tagtacgaca cttaaccgat cggggaattg gcgtcctcat
     2161 caccgatcac aatgtgcgcg aaactctgga cttaatcgac cgggcatata tcatctatga
     2221 cggccgtgtg ctgatagagg gcactcccgc cgaggtcgtc gcgaacgagg aggtgaagcg
     2281 cgtgtacctg ggcgatatgt tctcccggtg acggtcgcgt ccagcgtccg
//
LOCUS       DATMGX010001085         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_187364, whole genome shotgun sequence.
ACCESSION   DATMGX010001085 DATMGX010000000
VERSION     DATMGX010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187364"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VNR65_17805"
     CDS             <1..412
                     /locus_tag="VNR65_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20577.1"
                     /translation="HESITSEWSSGPASVRVPDSSRALRHFRKMPILLQKSAAADRRS
                     AISLRTAGIDLPTLTLFTRLQHYATHKARSGELAGPMNVHAPSLHRLMRTLANLGILT
                     EQAEGRFALTSLGEALKTGAPGSAKSAVIFAVSP"
     gene            440..604
                     /locus_tag="VNR65_17810"
                     /pseudo
     CDS             440..604
                     /locus_tag="VNR65_17810"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(858..1451)
                     /locus_tag="VNR65_17815"
     CDS             complement(858..1451)
                     /locus_tag="VNR65_17815"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HWJ20578.1"
                     /translation="MKQIGLFEMRRLEALSNTIFGVAMTLLAYDLPKASRFASAPAWM
                     DLVRAYAQPVIALTISFIVAGVFWLSHHRRLTFAPEGSRGEVFLNLIFLLSIVMLPVT
                     NGFYGMYRLDSVVAVSYGFHLTVIAGLNALLWILALRGRRNSELLATAVFPVFALVFA
                     TVMAAVAPFVAQFMWCLAFGAPLAGWLAARRWPPRTN"
     gene            1793..1954
                     /locus_tag="VNR65_17820"
     CDS             1793..1954
                     /locus_tag="VNR65_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20579.1"
                     /translation="MGCHRGLLRKPRGFPRVVDLRVLTRNPIERFFDTIKQCRRIASR
                     QDLAANYLA"
     gene            complement(2033..2392)
                     /locus_tag="VNR65_17825"
     CDS             complement(2033..2392)
                     /locus_tag="VNR65_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20580.1"
                     /translation="MSTRVSQVTPEIARHSPRNGLRLTPCSPWRSGFLVTIAPEKLAS
                     QELDASVEASGPHDFAVRKKSALVFGAACVHRILSRVRDDLEPPLQRDRTVADMKVIC
                     ANTEAEYFCARGWTGFC"
BASE COUNT          521 a          752 c          760 g          495 t
ORIGIN      
        1 gcatgaatcc ataacgtcag agtggtcgtc gggccccgct tcggtgcgcg ttcccgactc
       61 cagtcgggcg ttgcggcatt tccgaaaaat gccgattttg ttgcaaaagt cggctgctgc
      121 cgatcggcgg tcggccattt cgttgagaac ggccggcatt gatctgccga ccctgacgct
      181 ctttacgcga ctccaacact acgcgacgca caaagcccga tcgggtgagc ttgccggtcc
      241 catgaacgta catgcgccgt ccctgcatcg attgatgcgc actctggcca atcttggtat
      301 tttgacggag caggctgaag ggcgttttgc gctgaccagc ctcggcgaag ccctgaagac
      361 gggtgctccg ggatcggcaa aatcggcagt gattttcgcc gtgagtccct gagggcacag
      421 tggttgggac cggcgacact ccacatcccg gaaaatgctc gatatggcca agctgtccca
      481 gcttggcggc caagagcgca cagaagccga gtatggccat cttctcgcca aggccggctt
      541 tcgcctcacg cgcgtaatac caaccggttc ggcggcgagc atcgtcgaag cgtccgtggc
      601 ttgaacctgt tgtccgcttc ggggtcaatc gcgtcacttt ggcggtgtgc cggtcacttc
      661 cggtctccgc cgattagcgg acattcctag ggccagtcgg catgtctcaa gggtgcccag
      721 gatcggaagt cgcggttcat agttcaagtc acggggcaaa ggccatcaga cccgcatcaa
      781 cacgattttg cggcattacg agacccgttg ccggtcagcc attgcatacg aggaagcctt
      841 ggcagagatc gagctggcta attcgttctc ggcggccaac gccgggccgc caaccagccc
      901 gccagaggcg cgccaaaggc caggcaccac atgaattgtg caacgaatgg agcgacggcg
      961 gccatcaccg ttgcgaacac gagtgcgaag accggaaaga ccgcggttgc cagcaactcg
     1021 gaattgcggc gacctcgcag cgccagaatc catagcagcg cattcaggcc ggcaatgaca
     1081 gtcagatgga agccgtagct tactgcgacg acgctatcga gccgatacat accgtaaaat
     1141 ccattcgtca ccgggagcat gacgatcgac agcaggaaga tcagattgag gaagacttcg
     1201 ccgcggctgc cttccggcgc gaaggtgagc cgccgatgat ggctgagcca aaacacgccg
     1261 gcgacgataa agctgatcgt cagtgcgatg acaggttgtg cgtaggcacg gactaggtcc
     1321 atccaggccg gcgcgctcgc gaacctgctg gccttcggca gatcataggc gagcagcgtc
     1381 atcgcaacgc cgaagatggt gttgctcaac gcctccaatc ggcgcatttc gaaaagaccg
     1441 atctgcttca acttcgctcc ccctcaaagc cgccggacac gagacccgga catgctttcg
     1501 gctgtaggac atggattgcc ccttgtgggc aaagtaaccg gcccatcgca gcacacttct
     1561 tagattcaac aagtcgcgat gctttggaat gatcatcaaa gcacgttggt tgctattggg
     1621 ataggccgcc atgtccgagt tgggtcaatc gcgtcgactt tggcatgtcc gcgtcatgtc
     1681 cgcattgacg acaaccttgg aagcgttggt ggtggttttt tgccagttcg aacatgattc
     1741 aggcgctcaa accggggagt tgaatcatgc ggcattaact cacggagttt gaatgggctg
     1801 ccatcggggc ctccttcgta agccgcgtgg atttcctcgt gttgtcgact tgcgcgtcct
     1861 gacgcgcaat cccatcgagc ggttcttcga cacgatcaag cagtgtcggc gtatcgccag
     1921 ccgccaggac ctcgcggcca attacctcgc gtgagtcagt tatccgcgtc tcgctgctta
     1981 tgagtccgcg ccttggtcca aatccccctg ccaatgcacc cggacgggca aatcagcaaa
     2041 aacctgtcca gcccctcgcg cagaaatatt cagcttcagt gttcgcgcaa atcaccttca
     2101 tgtccgccac cgtcctgtcc ctctggaggg gcggctcgag atcgtcacgg acgcgggaca
     2161 ggatgcggtg gacgcaggcg gcgccgaaga cgagggcgct tttcttgcgg acggcgaagt
     2221 cgtgtggtcc tgacgcctcg acgctggcgt caagttcttg agaagcaagc ttctcagggg
     2281 cgatggtgac aagaaagccc gatcgccagg gagagcacgg agtaagccgt aaaccattgc
     2341 gcggggaatg ccgggcgatt tccggtgtga cctgactaac gcgtgtgctc actaccacta
     2401 tcgttgcaca cgcggctatc gggcgcatcg ggcgcccggc attccctgcg ccctctgatc
     2461 gaagagggcg gaacgagcag gccaaaactc gcgcgaaccg cgcggcgaga tcgcgaggtg
     2521 tggctgtt
//
LOCUS       DATMGX010001086         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_96163, whole genome shotgun sequence.
ACCESSION   DATMGX010001086 DATMGX010000000
VERSION     DATMGX010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96163"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(593..>2121)
                     /locus_tag="VNR65_17830"
     CDS             complement(593..>2121)
                     /locus_tag="VNR65_17830"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWJ20581.1"
                     /translation="LVAAANLSQRYIADRFLPDKAIDLMDEATSRLAMELESVPSEID
                     VVQRRLVQLELADRQLADETEEHAQQRRKEVQEEITELRKKLADLRKQWELEKSGLGN
                     VQDIRKNLIDAQLQYNQLAASIKEKQSAGSIVSEADYQKLYELDLQQKNLAGQLDTLE
                     SDAGKKPTNGTRRLLRKEVGPEEIAEVVSAWTGIPVSRMMETERAKLLVLEDRIHQRL
                     VGQDEAVEAVANAVRRNRSGLQDPNRPIGSFIFLGPTGVGKTELCKALAEVMFDNEDA
                     MIRLDMSEFMERHTVSRLIGAPPGYVGYEEGGALTEAIRRRPYSVILLDEIEKAHRDV
                     FNVLLQVLDDGRLSDNRGHTVDFTNTIIVMTSNIGSQVIQEIAKEGGTEEEMRDAVME
                     TLQTRLLPEFLNRVDEIMIFHPLQKGQIRKIVELQVARLRKQLTDKGIELDVTETALD
                     EIASEGYDPTNGARPLKRVIQQRIQNPLAVELLKREFEEGSRVKIDYLGGDFTFKRSN
                     "
BASE COUNT          397 a          640 c          577 g          507 t
ORIGIN      
        1 atgtggcgcg gttctgtttt ttacagctct ttctttgttg aactatgatc ttgtagcatt
       61 cgaagtaacg attctcacca taaactggat agtgctgatc gtttcaggtg tgctgctgag
      121 catcgtattt aggcgagctg ctcgtgggag ttctgtgtcg gcccgtgctg tttgaattgc
      181 gaagcgatgc gggaacgcgg aaactgcaac tgcacggtgt gttgttcaca cttacgtgca
      241 cgtattgcgc acagcggccg gggcctaata caggctccga cttcacaacc tacgtggcct
      301 ctcttggcat ccattacccg agcatggata tgatggttac ctgcgaaact gtagggatgt
      361 aactctcaca agtatttcat tgccaagcct tcttcaaaac acatagggga taagccgttt
      421 cgtcctgtgc gagtatgaca cataggcttc gccgagttct gcaattaaga gcctctcctc
      481 aatgtacctt cggtacccat agacgacctt gaatatcggc acgagcgcaa gtacagctcc
      541 ccacgactgt agggcaagtc cgaatcccac gaggaatctg accggtgacg tgttaattgc
      601 tccgcttgaa cgtaaaatcg ccaccgagat aatcgatctt cacgcggctg ccctcctcga
      661 actcccgctt gagaagctcg acggccaacg ggttctggat ccgctgctgg atcacccgct
      721 tcaaaggccg ggcgccgttg gtcgggtcgt agccttccga cgcgatttcg tctaaggccg
      781 tctcggtcac gtccaattcg atccccttgt cagttagctg cttccgcaac cgcgcgacct
      841 gaagctcgac gatcttgcga atctgtccct tttgcaaggg gtggaagatc atgatctcgt
      901 cgacccggtt gaggaactcg ggcagcaaac gggtttgcag cgtctccatc acggcgtcgc
      961 gcatctcctc ctcggtgccg ccctccttgg cgatctcctg gatcacctgg ctgccgatgt
     1021 tgctggtcat cacgatgatg gtgttggtga agtcgaccgt gtgaccccga ttatcgctca
     1081 accgcccatc gtccaacacc tgcaacagga cgttaaaaac gtcgcgatgg gccttttcga
     1141 tttcgtcgag aaggatgacg gaatagggcc gtcggcgaat cgcctcggtc aacgcgccgc
     1201 cttcctcgta gccgacgtag cccggcgggg cgccgatcaa tcgactcacg gtgtgccgct
     1261 ccatgaactc gctcatgtcc aagcggatca tcgcgtcttc gttgtcgaac atcacctcgg
     1321 ccagcgcctt gcacaactcg gtcttaccga cgccggtcgg tccaaggaaa ataaacgagc
     1381 caatcggccg gttcgggtcc tgcaagcctg accggttccg ccgcacagcg ttggccacgg
     1441 cctcgaccgc ttcgtcctga ccgacgagcc gctggtggat gcgatcttcg agcactaaga
     1501 gcttcgcccg ctcggtctcc atcatccgcg agacgggaat gcccgtccaa gcactgacaa
     1561 cctcggcaat ttcctccgga ccgacctctt tgcgcaagag ccggcgtgtg ccgttggttg
     1621 gcttcttgcc ggcgtccgat tcgagcgtgt ccagctggcc ggcaagattt ttctgctgga
     1681 ggtccaactc gtaaagcttc tggtaatcgg cctcggaaac gatgctgccc gccgattgct
     1741 tctccttaat cgatgcagct aactgattgt actgcaactg ggcgtcaatc aagtttttac
     1801 ggatatcctg cacgttgccc agcccgctct tttcaagctc ccactgtttg cgcaggtcag
     1861 caagcttttt gcgcagctcg gtgatctcct cctggacttc cttgcgacgt tgctgtgcat
     1921 gctcctcggt ctcgtcggcc agttggcggt cggcgagctc aagctgcacc agtcgccgct
     1981 gcaccacgtc gatctcactg ggtacgcttt ccagttccat cgctagccgg ctggtggcct
     2041 cgtccatcag gtcgatggcc ttgtcgggca gaaaccggtc ggcgatatag cgctgcgaaa
     2101 ggttggccgc tgccacgagg g
//
LOCUS       DATMGX010001087         2190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_312261, whole genome shotgun sequence.
ACCESSION   DATMGX010001087 DATMGX010000000
VERSION     DATMGX010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312261"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..455)
                     /locus_tag="VNR65_17835"
     CDS             complement(99..455)
                     /locus_tag="VNR65_17835"
                     /inference="COORDINATES: protein motif:HMM:NF018151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeA/HdeB family chaperone"
                     /protein_id="HWJ20582.1"
                     /translation="MKKFLLISALAAMATPGAAKADPVDLNAYVDANGFIKVQQLTCA
                     QLANTYQEDANALMAWYSGWYNGLAHKNEMDYKKGRELEHLVIEYCKAHPDEKIIHAI
                     AVNLKDMRAEGMMQEK"
     gene            complement(558..2105)
                     /locus_tag="VNR65_17840"
     CDS             complement(558..2105)
                     /locus_tag="VNR65_17840"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HWJ20583.1"
                     /translation="MFTKRDLLRSGALTAAGVAATNPGPVLAEMLTGAAADPKMKYST
                     AMPPGVTEPNTLETRFGKLNFFGGFPDNESVDKLYDNLDFQRAVQGYLLGLPVVSQVA
                     NRDAILAFGPANTTVPIWENLVDSQTIGLTFNDNTPYTWMWVDLHGGPLVIEAPPKVL
                     GLVDDIWYTWNGDIGITGPDKGEGGKYLLLPPGYKGEVPDGYFVIRPGSYSVWPVWRS
                     FLVDGDPKPGIDLVKKHLKIYRLADAANPPKLNYVNISGKPFSMDAPADYRFWELLSK
                     VIQDEPTDTVDATTLGFWASVGLMKGKPFAPDERMKKILTEAAAVGDATARAIMYRWR
                     GADGYWYPDTKSNWRLGFVGGYKFEENGARLLNAYSGFFFYATGITPAMDTRIVGEGS
                     QYMVGIVDSKGDVLDGGKNYKLRLPRDIPVKDFWSVIVYDNQTRSMLQTDQQFPSVSS
                     QTKGLKVNADGSVDVYFGPKAPAGEEKNWAQTMAGKSWNILLRLYGPLQPFFDKTWRP
                     GEIELEP"
BASE COUNT          426 a          649 c          617 g          498 t
ORIGIN      
        1 gaaggtcacc gtttgttggg cgctagcgct gctcgtcacg cctatagacg tcgcggcgca
       61 gaacgccgcc gatagaagca acttcttcat gatctcgctc acttttcctg catcatccct
      121 tcggcgcgca tgtccttcag attcacagcg atcgcgtgaa tgatcttctc gtccggatgc
      181 gccttgcagt attcgatgac gagatgctca agctcgcggc ccttcttgta gtccatctca
      241 tttttgtgag cgaggccgtt gtaccagccg ctgtaccagg ccatgagcgc gttcgcgtct
      301 tcttgatagg tgttggcgag ttgcgcgcag gtcagctgct gaactttgat aaagccattc
      361 gcgtccacgt aggcgttgag atcgacggga tcggccttcg ccgcgccggg cgtagccatc
      421 gcggccaggg ccgagatcaa taggaacttt ttcatttcaa actccggatg ttgcgttgca
      481 cggctggaat gaactgcatg tgaccttgaa accgtcgaga acgtcgaact gcttcgcatc
      541 tgcgtctctg aggcgcctca aggttcgagc tcgatctccc ccggacgcca cgtcttgtcg
      601 aagaaaggct gaagcggccc atagagacgc agcaatatgt tccagctttt gccagccatc
      661 gtctgagccc aattcttctc ctcgccagcg ggcgctttgg gaccgaaata cacgtcgact
      721 gagccgtcgg cgttgacctt caagcccttg gtctgactgc tgacactcgg aaattgctga
      781 tctgtctgaa gcatcgaacg cgtttggttg tcgtacacaa tcaccgacca gaaatccttg
      841 accgggatat ctcgcggcag acgcagcttg tagtttttac cgccatcaag gacatcgccc
      901 ttggagtcga caatgcctac catatactgc gatccctcgc cgacgatcct ggtatccatc
      961 gccggtgtga tgccggtcgc ataaaagaag aagccggaat aggcattcag caggcgcgcg
     1021 ccgttttcct caaacttgta gccgcccaca aaaccgagcc gccaattgct cttggtgtcg
     1081 gggtaccaat agccgtcagc tccacgccac ctgtacatga tggcgcgggc ggtcgcgtcg
     1141 cccacggccg ccgcttcggt cagaattttc ttcatccgct cgtcgggtgc gaaaggcttg
     1201 cctttcataa gtccgaccga ggcccagaag cccagcgtcg ttgcgtcgac cgtatcggtc
     1261 ggttcgtctt gtatgacttt gctcagcaac tcccagaaac gatagtcggc aggcgcatcc
     1321 atactgaatg gcttgccgga tatgttgacg taattgagct tgggcggatt ggccgcgtcg
     1381 gcgagccgat aaatcttcag gtgtttcttg acgagatcaa tgccgggctt ggggtcgccg
     1441 tcgacgagga agctgcgcca aaccggccaa accgaatatg aacctggtcg aatgacgaaa
     1501 tagccatccg gcacttcccc cttatagcca ggcggcagca gcaggtattt tccgccttcg
     1561 cctttgtccg gcccggtgat cccgatgtcg ccgttccaag tgtaccagat gtcgtcgaca
     1621 agtccgagca ccttgggcgg cgcctcaatg acgagggggc cgccgtgcag atcgacccac
     1681 atccacgtgt acggcgtgtt gtcgttgaag gtgagcccga ttgtttgcga gtcaaccaag
     1741 ttctcccaga tcggcacggt cgtattggcc ggaccgaacg cgaggattgc atcgcggttg
     1801 gcgacctggc tcaccacggg caaccccagc aagtacccct gcacagcccg ctggaagtcg
     1861 aggttgtcgt aaagcttgtc gacgctctcg ttatcgggga agccgccgaa gaaattcagt
     1921 ttcccgaagc gggtttccaa tgtgttgggc tccgtgacac ccgggggcat tgcagtcgaa
     1981 tacttcattt tcgggtcggc ggccgcaccc gtcagcattt cagcgagcac cggaccgggg
     2041 tttgttgctg cgacccctgc cgcggtcaac gcgccggagc gaagcagatc acgtttcgta
     2101 aacattctgg agctccgttg gctaagctaa tggatcttct gcatcacttc gcgggccgct
     2161 tcttgcggcc ctcgtggttc acaagtgaaa
//
LOCUS       DATMGX010001088         4574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_121699, whole genome shotgun sequence.
ACCESSION   DATMGX010001088 DATMGX010000000
VERSION     DATMGX010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121699"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VNR65_17845"
     CDS             complement(<1..617)
                     /locus_tag="VNR65_17845"
                     /inference="COORDINATES: protein motif:HMM:NF040245.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20584.1"
                     /translation="MTIHQTTIRPAVTPAEQRFLDLYASVSGELPGAGSAAVRAWRDA
                     ARDAFIMMGVPHRRVEEWKYTDLRALMPEVYPLGGLDARAPDVSVEAAIGKSLAHLDA
                     YRAVFVGGRFRPDLSTIHDAHGVTFEPLNQALEDDGASAQALAKLALPQRDVIAALST
                     AFATDGAILAVAPKTRLEKPIHLIFIATDDKPALYALQHAISIGE"
     gene            complement(627..1376)
                     /gene="sufC"
                     /locus_tag="VNR65_17850"
     CDS             complement(627..1376)
                     /gene="sufC"
                     /locus_tag="VNR65_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWJ20585.1"
                     /translation="MLEIKDLHAEIDGKEILRGFNLTVPKGEVHAIMGPNGSGKSTLA
                     YVLSGKEDYEVTGGSVTWDGKDLLAMSPDERSHAGVFLAFQYPMEIPGVASLTFLRAA
                     MNSQRKAHGKGEISIPDFMRTVRTEAEKLSMSMDMLKRPVNTGYSGGEKKRHEVLQMA
                     LLQPTLCILDETDSGLDIDAIRIAADGVNRLRSPDRAMVVITHYQRLLDYIVPDVVHV
                     MARGQILHSGGKELALELEETGYAAYRIEAA"
     gene            complement(1386..1652)
                     /locus_tag="VNR65_17855"
     CDS             complement(1386..1652)
                     /locus_tag="VNR65_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20586.1"
                     /translation="MTYWFKPKRYGWGATPANWRGWLATVGFIAIAYAFLAGIVAYRS
                     ELTVEAVIVWAVAFVALELAFIWIAWKKTNGDWRWRWGSKDIGK"
     gene            complement(1808..2272)
                     /locus_tag="VNR65_17860"
     CDS             complement(1808..2272)
                     /locus_tag="VNR65_17860"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HWJ20587.1"
                     /translation="MILRAFFAMAVVTAVAVPAYAEEAATCAPIAKADVERLFDRWND
                     SLKTGDPEKVADNYAEDAILLPTVSNKPRLTREERVDYFTEFLKKEPVGVIDSRVIKI
                     GCNTAVDAGLYTFTLHGHDLVHARYTFTYEFKDGKWGIAHHHSSAMPEKAGS"
     gene            complement(2409..2762)
                     /locus_tag="VNR65_17865"
     CDS             complement(2409..2762)
                     /locus_tag="VNR65_17865"
                     /inference="COORDINATES: protein motif:HMM:NF025453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HWJ20588.1"
                     /translation="MALILAGLLGVFISFLGVWFITSPFNAVQAFGVDPSHLADIALA
                     PGLGVRDVALGLIIIAFALARKPAATGTALLITSIVPIGDYVIAGKAFGYAGAIRHLI
                     ALPFLLILGLALVRK"
     gene            complement(2922..3308)
                     /locus_tag="VNR65_17870"
     CDS             complement(2922..3308)
                     /locus_tag="VNR65_17870"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HWJ20589.1"
                     /translation="MPLTRVALRRGKSAEYKRAILDGIYAAMRESFSVPEDDRFMLID
                     EYDAENIQYSYNYPGIAHSDDLLIIQLTVNNTRTVEQKKALYARIVEKLTENPGVRQE
                     DVLINLVEVAKENWSFGIGVAQYAHE"
     gene            complement(3327..>4574)
                     /gene="sufB"
                     /locus_tag="VNR65_17875"
     CDS             complement(3327..>4574)
                     /gene="sufB"
                     /locus_tag="VNR65_17875"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWJ20590.1"
                     /translation="KIDYEDLYYYSAPKSTEGPKSLADVDPELLRMYEKLGIPLKEQA
                     VLAGVEGAMVTGPRIAVDAVFDSVSVVTTFKEELGRAGVIFCSISEAVREHPELVRKY
                     LGTVVPTSDNYFATLNSAVYSDGSFVYVPEGVRCPMELSTYFRINEKNTGQFERTLII
                     ADKGSYVSYLEGCTAPMRDENQLHAAVVELVALDEAEIKYSTVQNWYPGDKDGKGGIY
                     NFVTKRGDCRGRNSKISWTQVETGSAITWKYPSCILRGDGSRGEFYSIAISNGRQQVD
                     SGTKMVHLGKNTSSRIISKGIAAGRSDNTYRGLVSAHRRATNARNFTQCDSLLVGDQC
                     GAHTVPYIEAKNASAQFEHEATTSKISDDQLFYCLQRGLSEEDAVALIVNGFVRDVLQ
                     QLPMEFLAETQKLISISLEGSVG"
BASE COUNT          874 a         1406 c         1329 g          965 t
ORIGIN      
        1 tcctcgccga tgctgatcgc gtgctggagc gcatacagcg ctggtttgtc atccgtcgct
       61 atgaaaatca gatggatcgg cttttcgagc cgcgttttcg gcgcgacggc gaggattgct
      121 ccatcggtcg cgaacgcggt gctgagcgct gcgataacgt cgcgctgcgg aagggcaagc
      181 ttcgcgagcg cctgggcgga agcaccgtca tcctccaacg cctgattcag cggctcgaag
      241 gtgacgccat gcgcgtcgtg gatcgtggac aagtccggac ggaaccggcc gccgacgaac
      301 accgcccgat aagcgtccag gtgagcaagc gatttgccga ttgccgcctc gacgctgaca
      361 tccggagccc tcgcgtcaag gccgccaagc gggtagactt ccggcatgag cgcgcggaga
      421 tcggtgtatt tccattcctc gacgcggcga tgcggaacgc ccatcatgat gaaggcgtcg
      481 cgggcggcgt ctctccaggc gcggacggcg gccgatcccg cgccgggcag ctccccggaa
      541 acgctcgcgt agagatcgag aaatcgctgc tccgcaggcg ttacggcagg ccggattgtg
      601 gtctggtgga tggtcatcgc cgccccttaa gccgcttcga tgcgataagc cgcatagcct
      661 gtttcttcca gctccagggc gagttccttg ccgcccgaat gcaggatttg cccgcgcgcc
      721 ataacgtgca ccacgtcggg cacgatgtag tccaagagcc gctgatagtg ggtgatgacg
      781 accatcgccc gatccggcga acgcagtctg ttcacaccat cggcggcgat gcggatggcg
      841 tcgatgtcga ggccagagtc cgtctcgtcc agaatgcaaa gcgtgggctg caacagcgcc
      901 atctggagca cctcgtggcg cttcttctcg ccgccggagt agcccgtgtt cacaggccgc
      961 ttcagcatat ccatggacat gctgagcttc tccgcctcgg tgcggaccgt ccgcatgaaa
     1021 tcggggattg aaatctcgcc cttgccgtgc gccttccgct gcgaattcat ggcggcgcgc
     1081 agaaacgtca gcgaggcgac gccgggaatt tccatcggat attggaaggc gaggaagacg
     1141 cctgcatggg agcgctcgtc cggggacatg gccagcagat ccttgccgtc ccaggtgacg
     1201 gagcctcccg tgacctcata gtcttccttg cccgaaagca cataagccag cgtggatttg
     1261 cccgagccgt tcggccccat gatcgcatgg acttcaccct tcggcacggt cagattgaag
     1321 ccgcgcagga tctccttgcc gtcgatttcc gcatgcaggt ctttgatttc gagcatgttt
     1381 tctctctact tcccaatgtc cttggaaccc cagcgccacc gccagtcccc gttcgttttc
     1441 ttccaggcaa tccagataaa cgcgagctcg agagcgacaa aagcgacggc ccaaacgatg
     1501 acggcttcga ccgtcagctc gcttcgataa gccacaattc ccgcgaggaa ggcgtaggcg
     1561 atggcgatga agccaaccgt cgcgagccat cccctccaat tcgcgggagt cgcgccccag
     1621 ccataccgct tcggcttgaa ccaatatgtc atttgcgcac cagcatcagg cgattgcgtg
     1681 gacttcaccc ttcggcgccg tgaggttgaa gccgcgcagg gtttccttgc cgtcgatttc
     1741 cgcgtgcagg tctttgattt cgagcatctc ttcctcttac tcgccgaaac cgacggcttt
     1801 gaacttctta gcttccggct ttctcaggca tggccgacga gtggtgatgg gctatgcccc
     1861 acttcccgtc tttgaactca taagtgaagg tatagcgcgc gtggaccagg tcgtggccgt
     1921 ggagcgtgaa ggtgtagagg ccagcgtcca ccgccgtgtt gcacccgatc tttatgaccc
     1981 gggagtctat gacaccgacc ggctccttct tcaggaactc ggtgaaatag tcgacccgct
     2041 cttccctggt cagccggggc ttgttcgata ctgtcggcag aaggatcgca tcctcagcat
     2101 agttatccgc gactttctcg ggatcgccgg tcttcagcga gtcgttccag cgatcgaaca
     2161 gcctctcgac atccgccttc gcgatgggag cgcaggtcgc ggcctcctcc gcgtaagccg
     2221 gaaccgccac agccgtcacg accgccattg caaagaacgc tcttagaatc atagtcgtct
     2281 cctatctgtt tgctcccaag caaacgacac cgttagcttg ggtatgcgac cattagaggc
     2341 gactgacgtt acctgaggcg tttccggcag gctggattcc aaaaagctgt tcatcatgag
     2401 cgccctccct atttccgaac cagagcgagg ccgaggatca gcaggaaagg cagcgcgatc
     2461 agatgcctga tggcgccggc gtacccaaag gcttttccag cgatgacata gtcgccgatg
     2521 ggcactatgg aggtaatgag gagtgcggta ccggtcgctg ccggcttccg ggccaaggcg
     2581 aatgcgatga tgatcagccc cagcgcaacg tctctgacgc ccagtcccgg agcgagcgcg
     2641 atgtcggcca gatgggaggg gtcgacgccg aacgcctgca cggcgttgaa cggcgacgtg
     2701 atgaaccaca cgcccaggaa gctgatgaac acgcccaata atccggccag aatcagcgcc
     2761 attttagctc cgtgtcatgc ccgggctcga cccggccatc cagggtgctt tcacaaattg
     2821 tctcgtagag cgtttcgttt ctctggcttg caagaacata gacaaaatag cggcccctcg
     2881 tggttgcttc tggatggccg ggtcaggccc ggccatgaca attactcatg cgcatattgc
     2941 gcgaccccga tgccgaacga ccagttctct ttcgccacct cgaccagatt gatcaagaca
     3001 tcttcttgcc tcacacctgg gttttcggtc agcttctcta caatgcgagc atacaaggcc
     3061 tttttttgct cgaccgtgcg agtgttattc acggtaagct gtatgatcag gagatcatcc
     3121 gagtgtgcga tgccgggata attatagctg tattgtatgt tctcggcgtc atactcgtcg
     3181 atcaacataa acctgtcatc ttccggcacg ctgaaacttt cacgcatagc ggcatagatg
     3241 ccgtcgagga tcgcacgctt atactcggcc gattttccgc gcctgagcgc gacacgtgtt
     3301 agaggcattg gttgttctcc cttcttttac cccacgctgc cttcgaggct gatcgagatc
     3361 agcttctgcg tctccgccag gaactccatc ggaagctgct ggagcacgtc gcgcacgaag
     3421 ccgttcacga tgagcgccac cgcgtcttcc tcggacagcc cgcgctgcaa gcagtagaag
     3481 agctgatcgt cggagatctt cgacgtcgtc gcctcgtgct cgaactgagc gctcgcattc
     3541 ttcgcctcga tgtacggcac ggtgtgcgcg ccgcactgat caccgacgag caagctgtcg
     3601 cattgcgtga agttgcgtgc gttcgtggca cggcgatgcg cggaaacgag cccgcgatag
     3661 gtattgtcgg agcgccccgc cgcaatgccc ttcgagatga tgcggctcga cgtgttcttg
     3721 ccgagatgca ccatcttggt gccgctatcc acctgctggc ggccgttcga gatggcgatg
     3781 gagtagaact cgccgcggct gccgtcgccg cgcaggatgc agcttggata tttccaggta
     3841 atcgccgagc ccgtttccac ctgcgtccat gaaatcttcg agttacggcc gcggcaatcg
     3901 ccacgcttcg tgacgaaatt atagatgccg cccttgccgt ccttgtcgcc ggggtaccag
     3961 ttctgcaccg tcgagtattt gatttcggcc tcgtcgagcg cgaccaattc cacgacggcg
     4021 gcgtgaagct ggttctcgtc gcgcatcggc gccgtgcagc cttcgagata gctcacatac
     4081 gagcccttat cggcgatgat gagtgtgcgc tcgaattggc ctgtgttctt ttcgttgatg
     4141 cggaagtagg tcgaaagctc catcgggcaa cggacgccct cgggcacata cacgaacgag
     4201 ccgtcggaat agaccgccga gttcagcgtc gcgaaatagt tgtcactggt tgggaccact
     4261 gtgccaagat atttgcgcac gagctcggga tgttcgcgca cggcctcgga aatagagcag
     4321 aaaatcacgc ctgcgcgtcc cagctcttcc ttgaaggtcg tcacaacgga aacgctgtcg
     4381 aacacggcat ccactgcgat gcgcgggccg gtcaccatcg cgccctcgac gccagccagc
     4441 acggcctgct ctttcagtgg aatgccgagc ttttcgtaca tgcgcaggag ctcaggatcg
     4501 acatccgcca ggcttttcgg cccctcggtc gatttcggcg ccgagtagta atagagatcc
     4561 tcgtaatcga tctt
//
LOCUS       DATMGX010001089         2023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_363681, whole genome shotgun sequence.
ACCESSION   DATMGX010001089 DATMGX010000000
VERSION     DATMGX010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363681"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1942)
                     /locus_tag="VNR65_17880"
     CDS             complement(<1..1942)
                     /locus_tag="VNR65_17880"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWJ20591.1"
                     /translation="MALVPGTKLGRYEILSPLGAGGMGEVYRARDTRLERTVAIKILA
                     SRLCAEPEAKERFEREAKAISSLNHPNICRLYDVGEQNEINFLVMEYLEGETLAQRLQ
                     KGPLAMYDILNTAVALADALTVAHAAGVIHRDFKPANLFLTHRNEVKILDFGLAKSAA
                     IIGGQSASAVADEVTMSIAPQLTIEGAALGTVAYMSPEQARGENVDVRTDLFSFGVVL
                     YEMTTGRRPFNGPTAAVVFDAILNRQPLTPSILNAAVFPSLERIITRLLAKDRSKRFQ
                     HAQDLLDDLRGIGKRATSNQPHNAADSGSIAVLPFEDLSPDRSQQAFCEGMAAEIINV
                     LGAIQGLRVISRTSAVRCHDKGMDIAEIAQHLNVQTVLEGTVRKSGARLRVTAELISS
                     CEGSQIWSERYDRSEGDVFDIQEEIANAIVKNLRGRLMGGQSPTVRRVTDNVDAYKLY
                     LKGRYAWERRNRAALQNAITYFEQAISADPDYALAHAGLADCYMIMGVYSIRPTREVL
                     PRALQLALRALELEPDLAEAHHSLGAVKHFLQWDWTGANACYTRALELDPRLTVTRGW
                     LSTLYVVSMMRRQDALTESVHAMKLEPDSGLIAYIAAINHYWARDPDGAAELIERALE
                     LEPQAVFAHWVRALIFSVKGHHD"
BASE COUNT          434 a          580 c          552 g          457 t
ORIGIN      
        1 cgtcgtggtg tcccttgacc gagaagatta gtgcgcgaac ccagtgggca aagacggcct
       61 gaggttcgag ctcaagcgcg cgttcgatga gttccgccgc accgtctggg tcgcgagccc
      121 agtagtggtt tatggcggcg atataagcga tgagacctga atcgggttca agcttcattg
      181 catggacaga ttcggtaagc gcatcctgtc gtcgcatcat gcttacaaca tagagagttg
      241 atagccaacc cctagtgaca gtaaggcgcg gatccaattc aagggcgcgc gtgtaacatg
      301 catttgcgcc agtccaatcc cactgcagaa agtgttttac cgcacctagc gagtgatggg
      361 cttccgccag atcgggctcc aactccagcg cgcgcaacgc caactggaga gcccttggta
      421 gtacctcgcg ggtgggcctg atcgagtaga ctcccatgat catgtagcag tcggccaaac
      481 cggcgtgggc tagagcataa tcgggatcag cggaaatagc ctgctcgaaa tacgtgattg
      541 cgttttgcaa cgctgctcgg ttgcggcgtt cccaagcata ccgcccttta agatacagtt
      601 tgtaagcatc aacgttgtcg gtgacacgcc tgaccgtggg cgattggccg cccataagcc
      661 tgcccctcag attctttact atcgcgtttg cgatctcctc ctgaatatca aagacatcac
      721 cctcgcttcg gtcgtaacgc tctgaccata tctgcgatcc ttcgcagcta ctgataagct
      781 cagccgtaac tcggagtcgc gcgcccgact tcctgacggt gccctccaac actgtctgga
      841 cattaaggtg ctgagcgatt tcggcgatgt ccatgccctt atcgtggcag cggaccgcag
      901 atgttctgga aatgacccgc aagccttgta tagctcccaa tacattgata atctctgccg
      961 ccatcccctc gcagaaggcc tgctgactgc gatcagggct cagatcctcg aacggcagca
     1021 cagcaattga cccactgtca gctgcgttat gtggttgatt acttgtcgct cgttttccga
     1081 ttccgcgaag atcgtccaac aagtcttgag cgtgctgaaa tcgctttgaa cggtctttcg
     1141 ccagtagacg cgtgattatc ctttctaacg atgggaatac ggcagcattc aagatgcttg
     1201 gagtcaatgg ctgccgattc aaaatagcgt cgaagacgac agccgccgta ggcccgttaa
     1261 atggtctccg gccggtcgtc atttcataaa gaaccacgcc gaatgagaat aggtctgtgc
     1321 gaacatcgac gttttcaccg cgcgcctgct caggcgacat ataagcgacg gttccaagtg
     1381 cagccccttc aattgtgagt tgcggcgcga tgctcatcgt gacctcgtcc gcgaccgccg
     1441 atgcgctctg cccgccgatt attgcagcgc tcttagcgag tccgaagtca aggatcttca
     1501 cctcgttgcg gtgcgtgaga aacaagttgg cgggtttaaa gtcccgatga attacgcccg
     1561 ccgcgtgcgc cacagttagc gcatccgcaa gcgcaacggc agtgtttagg atgtcataca
     1621 tcgccagcgg ccctttttgg aggcgctgtg ccagagtctc gccttcgaga tactccatca
     1681 caaggaagtt gatctcgttc tgctcgccta cgtcgtaaag tcgacaaatg ttggggtgat
     1741 tgagcgagga aatagccttg gcttcgcgct cgaagcgttc tttcgcctcg ggttcggcac
     1801 acaggcggga cgccaaaatc ttgatggcaa ccgtgcgctc caaccgcgtg tcacgggcgc
     1861 ggtagacctc tcccattccc cccgctccga ggggcgacag aatttcgtag cgcccgagtt
     1921 tcgtgccagg gactagagcc atccgtgcgc gccattatag tccgacgcag cacgctgcag
     1981 cgggatccta cgattctgga taaatggact cggaagtaat cca
//
LOCUS       DATMGX010001090         2942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_388647, whole genome shotgun sequence.
ACCESSION   DATMGX010001090 DATMGX010000000
VERSION     DATMGX010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388647"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VNR65_17885"
     CDS             complement(<1..414)
                     /locus_tag="VNR65_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20592.1"
                     /translation="MTAEPDDTPVEPSIAPAAFGKASDQSPRPGPTLIEQQPEAQPAG
                     LGALLRPLAAGLIGGLLGGGILALLLAGGGPKPDDEARLQIKELQDKFREQQDKTAQL
                     NDTVRVKLAAPVKAASPEELSEVRSRLDDLSKATKA"
     gene            complement(511..1224)
                     /locus_tag="VNR65_17890"
     CDS             complement(511..1224)
                     /locus_tag="VNR65_17890"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWJ20593.1"
                     /translation="MRLLVTRPEPDASTFAEELRGLGHEPVLQPLIEFRVLEFDLEPL
                     LAAEVLIITSVNSLRALQERGVINDLLSKPLYCVGEQTAKQALAVGFETVLEIAQTGE
                     ELAGKIIASGRRDAPLLHIMGEHMAFDIVGALAREGFSVHSVTVYSMEACREFSPAID
                     AMLKAGGIDGVILMSPRTAEIYVSLCHRHGIADCAKSPLYFCLSRNVAAKLASLKPNH
                     VHVSGKPNRMALLELLLAD"
     gene            complement(1271..2209)
                     /gene="hemC"
                     /locus_tag="VNR65_17895"
     CDS             complement(1271..2209)
                     /gene="hemC"
                     /locus_tag="VNR65_17895"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWJ20594.1"
                     /translation="MKNLSEARQIRIGTRGSALALAQAHEVRDRLTAIYGEKVSFEIV
                     IIKTTGDKILDRALAAVGGKGLFTKELEEQLLDNTIDIAVHSMKDMQAELPNGLEVGC
                     VLPREDVRDAFISPVAKSLADLPSGSIVGTASIRREAFIRNRRPDLKMILFRGNVQTR
                     LRKLEEGEAHATLLASAGLRRLGLADRITNVISVEEMLPAPAQGAIGIELRSNDDVTK
                     EIISAINDRDSFIAVEAERAFLSQLDGSCRTPIAALARVDGDALSFKGIILTPDGARI
                     YETDRKGDVKDAAALGKDAAHELYERGGKDVFRDVG"
     gene            2348..>2942
                     /locus_tag="VNR65_17900"
     CDS             2348..>2942
                     /locus_tag="VNR65_17900"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20595.1"
                     /translation="MQQRAINKEHSGAAATKNRELTVLGIETSCDETAAAIVRRDTQG
                     NGAILSNIVRSQFTDHAPYGGVVPEIAARAHVQVLDGLIEAALAEASLKFGDLDGIAA
                     TAGPGLIGGLLAGFVTAKAIALVHNLPIAAVNHLEGHALTVGLTEGLHPPYLLLLVSG
                     GHTEFLTVRAHFDYQKLGGTIDDALGEAFDKVAKMLRL"
BASE COUNT          552 a          872 c          899 g          619 t
ORIGIN      
        1 agccttcgta gccttagaga gatcgtccag acgtgagcgc acctcactca gttcttccgg
       61 gcttgccgcc ttcactggtg cagcaagctt cacacgaacc gtgtcgttca gttgagccgt
      121 tttatcctgc tgctccctga acttgtcttg cagttccttt atctgaagcc tcgcctcgtc
      181 gtccggcttc ggaccgcctc ccgccaggag cagagccagg atgcctccac caagaaggcc
      241 gccgatgagg ccggctgcca gcggacgaag aagcgctccc aggcctgcgg gttgcgcctc
      301 aggctgctgt tcgatcaaag ttggtcccgg gcgaggcgac tggtcgcttg ccttgccgaa
      361 ggcagcgggg gcaatactcg gctcgaccgg agtgtcatcg ggctcggcag tcatcttgat
      421 cctcggtctt ggtcgtttct cgtcagccat cgttataagc gacctttgtt gcggccttgt
      481 tgcagtatct tacgcatggg cgcgacgata tcaatccgcc agcagaagtt ccaggagcgc
      541 catccgattt ggtttccctg aaacatgcac gtgatttggc ttgagggaag caagcttggc
      601 ggctacgttt cttgataaac agaaatataa cgggctttta gcgcaatcgg cgatgccgtg
      661 cctgtgacat aatgaaacgt agatttctgc cgtgcgcggc gacatcagga tcaccccatc
      721 gatgcccccg gccttcaaca ttgcgtctat tgcgggggag aattcgcggc aagcctccat
      781 actgtaaacg gtcacgctat gaacggaaaa gccttcgcgg gcgagcgcgc ccacgatgtc
      841 gaaggccatg tgctctccca tgatatgaag gagcggcgcg tctcttctgc cagacgcaat
      901 aattttcccg gccagttctt cgccagtttg ggcaatttca agcacagtct cgaagccgac
      961 cgcaagtgcc tgcttcgccg tttgttcgcc aacgcagtaa agcggcttgc tgaggaggtc
     1021 gttgatgacg ccccgctcct gcagcgcgcg caggctgttg acgctggtga tgatgaggac
     1081 ctcggcggca agcaagggtt ccaaatcgaa ttcgagaacg cgaaactcaa tcagcggctg
     1141 gagaacgggt tcgtgaccga gtccgcgaag ctcttccgcg aacgtgctgg cgtcgggctc
     1201 aggtctcgtc acaaggaggc gcatacttcc tccagccaag catgaatcca ggttacaaga
     1261 gcgtttccgc ctaaccgacg tcgcggaaga cgtccttgcc gcctcgctcg tataactcgt
     1321 gcgcggcgtc cttgccaagc gcggccgcgt ccttcacgtc gcctttccgg tcggtctcgt
     1381 agatgcgcgc gccgtccggc gtgagaatga tgcccttgaa ggatagcgcg tcgccgtcga
     1441 cgcgggcgag cgccgctatc ggcgtccggc aggaaccgtc gagctggctg aggaatgcgc
     1501 gttccgcctc gactgcgatg aagctatcgc gatcgttgat ggcggaaatg atctctttgg
     1561 ttacgtcatc gttggagcgc aactcgatgc cgatggcgcc ctgggccgga gccggcagca
     1621 tctcctcgac ggaaatcacg ttcgtgatgc gatcggcgag cccgagacgc ctcagcccag
     1681 ccgatgcgag cagcgttgcg tgagcctctc cttcttcgag tttgcggagc cgcgtctgga
     1741 cgttgccgcg aaacaggatc atcttcagat cgggccgtct gttgcggatg aaggcttcgc
     1801 gccggatgga cgccgttccc acgatcgagc ccgaaggcag atccgccaac gatttagcca
     1861 ccggagagat gaaggcgtcg cgaacgtctt cgcggggcag aacgcagccg acctccagcc
     1921 cgttcggcaa ttcggcttgc atgtccttca tggaatggac ggcgatgtcg atggtgttgt
     1981 cgagaagctg ctcttccagt tccttggtga agagcccctt gccgccgacg gccgccaagg
     2041 cccggtcgag gatcttgtcg ccggtcgtct tgatgatgac gatctcgaag gagacctttt
     2101 cgccgtagat ggctgtcagc cggtcgcgca cctcgtgtgc ctgtgcgaga gcgagagcgc
     2161 tccccctggt gccaatgcga atttgccttg cttctgagag atttttcatg gttgtctgca
     2221 cttgggttgt acgggcgcgc cgtcagtgac cggctgagat gagggtgcgt gtcgcgatgg
     2281 ggacactaag catatccggg ccgtgacgta cagcacgaaa gcgatttagg gatctacata
     2341 aaggatcatg cagcaacgcg cgatcaataa agagcattcg ggagcggcag cgacgaaaaa
     2401 tcgcgaatta accgtacttg gtattgaaac gagctgcgac gaaactgccg ccgcaatcgt
     2461 aaggcgcgac acgcaaggca acggcgcaat cctctcaaat attgtgcgat cgcaattcac
     2521 cgatcacgcg ccctatggcg gtgtcgtgcc ggagatcgcg gcgcgcgctc acgtgcaggt
     2581 gctcgacggg ctgatcgagg cggcgctcgc agaagccagc cttaagtttg gcgatctgga
     2641 tggcatcgct gccaccgccg ggccgggcct catcggcggc ctgcttgcgg gtttcgtgac
     2701 cgctaaggcg attgcgctcg tgcataacct gccgatcgcc gccgtcaatc acctcgaagg
     2761 tcatgccctg acggtcggcc tgacggaggg tctgcacccg ccctatctcc ttcttcttgt
     2821 ttcgggcggt cacaccgagt tcctgacggt gagggcgcat ttcgactatc agaagctcgg
     2881 tggcacgatc gatgacgcgc tcggcgaagc cttcgacaag gtggccaaga tgctgcgcct
     2941 cg
//
LOCUS       DATMGX010001091         4376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_97805, whole genome shotgun sequence.
ACCESSION   DATMGX010001091 DATMGX010000000
VERSION     DATMGX010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97805"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VNR65_17905"
     CDS             <1..867
                     /locus_tag="VNR65_17905"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20596.1"
                     /translation="LFTPLKHRTLEIGLRDWVRDQTGVDLGYVEQLYTFGDRGRHARP
                     GDEGLHVVSIGYLALTRNEEAPRSSGVKWSAWYAHLPWEDWRNGKPEILTQEIEPRLE
                     EWAAQPETSGAPRRPLARMDRLRMCFGQSVPWDDEKVLDRYELMYEAGLVAEAARDGR
                     AAAQHWSGLPPLGRQMMFDHRRILATAMSRLRGKLKYRPVIFELMPETFTLFDLQRVV
                     EAILGSYLHKQNFRRLVENAGLVEPTGQTAARTGGRPARVYRFRRNVLLERPAPGFRV
                     KVQHVVRTTPTC"
     gene            1209..1772
                     /locus_tag="VNR65_17910"
     CDS             1209..1772
                     /locus_tag="VNR65_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWJ20597.1"
                     /translation="MPEKTASQRRFGFETNQDVVYPTHGVGRIVAIEEQEIAGCKLEL
                     FVINFEKDKMTLRVPTSKSQSAGMRKLSDDAVMTRALDTLRSRARVKRTMWSRRAQEY
                     EAKINSGDLITIAEVVRDLYRSDSQPEQSYSERQLYEAALDRMAREIAAAQDVDESIA
                     VQKVAEALTKGRQLQRKREEYDRKEAA"
     gene            2092..>4376
                     /locus_tag="VNR65_17915"
     CDS             2092..>4376
                     /locus_tag="VNR65_17915"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HWJ20598.1"
                     /translation="MAIRRAGPTFWDRLAHEIEGDAASNLFTRGRYATDASMYQCFPA
                     GVACPKSANDVAVIVEMAREEGLPVIARGGGTSTAGQALGEGIIVDLSKYLTGIVEID
                     AENRRCAVEPGCSPAALAAALEAHGLFFPVEIASAAQATIGGMLGNNSSGLRAMRYGS
                     MRDTIACADAFLADGQRTIFSPYAEADKQSFPGRDRLLDLLHFGEIHGKAIGTHWPLR
                     APGTPEPEGYDLRTLLAYSEDQNLARLLAGAEGTLAIATRIDLKLAKRPENRALGVCR
                     FANLGRALRAVPKIALLNPAAIELLDKTMLGFVAEQAKSDIQAARLMRGVPEALLIVE
                     FDEDNPVDNTRFLKALADVAAEVDKGKFSVIEVIGENARAALWRLRRDALALAWTLKS
                     AAQPLSFLEDAAVPLHQLAAYGAELETLLARHGIRTAIYGQAGRGSLQVRPILDLRHA
                     QDRKRMRTLSDEMAVLLRAHEGVMTSGYGLGLARSEALERALSPEAIALFKDLKTQLD
                     PGFLLNPGKILRAPNFDDNAFLRATLEERHDGAATALSWGTASSPAQGHASRCSGLGH
                     CRSAEHNFTCPSHAVTRDERDSPRGRANVMRLALSGQLGDGALASAPMLEAMKLCVSC
                     KTCTVACPFGVDIPKLKVEVLAAARAAGDGSRALDLFARLPEYTDRARGWRFLIAMRD
                     LFPGLPRLSERHFGLAADRPWPRWSGRRFKSPHKAEPGPAGLVAIFADTFNRSFEPAN
                     LRAAAHVLKAAGYGVIAFTDE"
BASE COUNT          881 a         1267 c         1330 g          898 t
ORIGIN      
        1 ttgttcacgc cgctcaaaca tcggactttg gagatcgggc ttcgcgactg ggtgcgagat
       61 caaactggcg ttgatctggg ctatgttgaa caactgtaca cctttggaga ccgtggacgc
      121 cacgccaggc cgggagatga aggtcttcac gtcgtttcaa ttggctactt ggccctgacg
      181 cgcaacgagg aggcgccacg gagcagcggg gtgaaatgga gcgcgtggta tgcccacctt
      241 ccctgggagg attggcgcaa tgggaagccc gaaatcctca cgcaggagat tgagcccagg
      301 cttgaagagt gggcggcaca gcctgaaacg agtggcgccc cacgccgtcc gctggcccgc
      361 atggaccggc ttcgcatgtg cttcggccaa tccgtgcctt gggacgacga gaaggttctg
      421 gatcgttacg aacttatgta tgaggctggg ttagtagccg aagcagcccg agatggtcga
      481 gcagcggcac aacactggtc gggactgccg ccgcttggaa ggcagatgat gtttgatcac
      541 cgccgcatcc tggccacagc catgagcagg cttcgcggga aactcaaata caggccggtc
      601 atttttgagc tgatgcccga aacgttcacc ctcttcgatt tgcagcgggt cgtggaggct
      661 attcttggaa gctacctgca caaacaaaac tttaggcgac tcgtggaaaa cgcgggcctc
      721 gtcgagccga caggacaaac tgctgcacgc accggtggcc ggccggcccg ggtttatcgc
      781 tttcggcgca acgtgttgct ggagcgtccc gcgcccggat tcagggtcaa agttcagcac
      841 gtggtgcgta caactccaac ctgttgaccg ttgctttgtt aacactatcc ttaggaatca
      901 agggggattc atgcgattct cggggattaa cctctttgaa agcttcctaa aattggcgca
      961 ttttgataat atttaaaggt cggtgaaaaa agtttcacgc gcagaggttg attcttcaga
     1021 cagcttcagc gaggaccata aagcaggcgc cgagcaaacc gcctgctaat gtatcactat
     1081 tcaggcatgg cttgaatcgg ctccttgggg ctggacgact atttattccc ggtaacgctg
     1141 tgaggcgcaa accgtaccga aattgagatg agcatcatct gccaagacta acaaaagtga
     1201 gagcgagtat gcctgagaag acagctagcc aacgacgctt tggcttcgag accaaccagg
     1261 atgtggttta tccgacgcat ggtgttgggc gtatagtcgc gatcgaagaa caggaaattg
     1321 ccggttgcaa gctcgagctt tttgtcatca atttcgaaaa agacaagatg accctccgcg
     1381 tgcccacctc gaagagtcaa agtgcgggca tgcggaaact ttcggacgac gcggttatga
     1441 ctcgcgcctt ggataccctc cgcagccgcg cgcgagtgaa gcgtaccatg tggagccgcc
     1501 gcgctcagga gtatgaggcg aagatcaatt cgggcgacct tatcacgatc gcggaggtag
     1561 tccgggatct ctatcgctct gattctcaac cggaacagtc gtactctgag cgccagcttt
     1621 atgaagctgc cttggatcgt atggcgcggg aaattgccgc agcgcaggat gtggatgaat
     1681 ccattgccgt tcagaaggtc gccgaggctt tgaccaaggg taggcaactc cagcgtaagc
     1741 gggaagagta cgacaggaaa gaagctgcgt aatcactcga ttcgtctttt atttcgggtt
     1801 gtctcgcgag gccgatttag cttcgtgcgc aagtagtgat ggctttgtcg gttattggac
     1861 ttttcccctc ttggcacatc caacgatcct gcgcaaatcg gtttagatga aacgttaccg
     1921 cagttcataa acagcctgac cgcggttttt cgagaggtac gacggatctt aaaacctgat
     1981 gggatcttct ggttgaacat tagcgacggt tacactagcg gtaatcgggg atggagggct
     2041 cgtccctagc cttgccggcg atgggtctct caccgcccgc accgagggaa aatggcaatc
     2101 cgaagagctg gacccacgtt ctgggacagg cttgcgcacg agatcgaagg cgacgccgca
     2161 agcaacctct tcacgagggg ccgctacgcc accgatgcgt ccatgtatca gtgcttcccg
     2221 gctggcgtgg cttgccccaa aagcgcgaac gatgtggccg tcatcgtcga gatggcccgt
     2281 gaagaaggct tgcccgttat cgcgcgcggc ggcggcacgt cgaccgcggg acaagcgctt
     2341 ggcgaaggca tcatcgtcga tctttctaaa tacctgacgg gtatcgtcga gatcgacgcc
     2401 gaaaaccgcc gatgcgcggt tgaaccggga tgctcgcccg ccgctttggc agcggcgctg
     2461 gaagcgcacg gtcttttctt tccggttgag attgcgtccg ccgcccaggc cacaatcggc
     2521 ggcatgctcg gaaacaattc gagcggcctg cgagccatgc gctacggctc gatgcgcgac
     2581 accatcgctt gcgccgacgc ttttcttgca gacgggcagc gcactatctt tagcccctac
     2641 gccgaggccg acaagcagtc cttcccgggg cgcgaccgtc ttctcgacct tctgcatttc
     2701 ggagaaattc atgggaaggc gatcggaacc cactggccgc tgcgcgcgcc gggaacgccg
     2761 gagcctgaag gctacgacct ccgcaccttg ctcgcgtatt cggaagatca gaatttggcc
     2821 cgcctcctgg ccggagccga gggcacgctc gcaatcgcga ccaggatcga cttgaagctt
     2881 gcgaaaaggc cggagaaccg ggcgctcggt gtttgccgtt tcgccaatct tggccgcgcg
     2941 cttcgggccg ttccgaaaat tgcgcttctc aatccggccg ccatcgagct tcttgacaag
     3001 accatgctcg gctttgtcgc agagcaggcg aagtccgaca tccaggctgc acgtctcatg
     3061 cgaggcgtgc ccgaggctct cctgatcgtc gagttcgatg aagacaatcc ggtggacaat
     3121 acgaggttcc tgaaagcgct cgccgatgtc gccgccgaag tggacaaagg caagttcagc
     3181 gtcatcgaag tcattggcga gaacgcgagg gcagcgctgt ggcgcttgcg ccgcgacgcg
     3241 ctggctctgg cgtggacctt aaaatctgcg gcgcagccct tgtcattcct ggaggacgcg
     3301 gccgttccgc ttcatcagct tgccgcgtat ggtgccgagc ttgagacgct tttggctcgt
     3361 catggtatcc ggaccgcaat ctatggccag gcagggcggg gcagccttca ggtccgcccg
     3421 atcctcgacc ttcgccacgc tcaggaccgc aaacgcatgc ggacgctctc ggatgagatg
     3481 gcggtgcttc ttcgcgcgca tgagggtgtc atgacatccg gctatggcct cggccttgcg
     3541 cggagcgagg cactcgagcg cgcgctcagc cccgaggcca tagctctctt caaggatcta
     3601 aagacgcagc ttgatcccgg cttcctcctt aatcctggaa aaatccttcg cgctccgaat
     3661 ttcgatgaca acgcgtttct tcgggcaacg cttgaagaga gacacgacgg cgccgccact
     3721 gccttgtcgt ggggcaccgc atcctcacct gcgcagggtc atgcaagccg gtgctcgggc
     3781 ctcggtcatt gccgctcggc ggagcataat ttcacctgcc catctcacgc cgtcacccgt
     3841 gacgaacgcg acagcccgag aggacgcgcc aacgtgatgc ggctcgcgct ttcgggccaa
     3901 cttggcgatg gagcgctcgc ttccgcgccg atgctcgaag cgatgaagct ttgcgtgtcc
     3961 tgcaagactt gcaccgtagc gtgcccgttc ggcgtcgaca tcccgaaatt gaaggttgaa
     4021 gtacttgcag ccgcgagagc agcgggtgat ggatcacgcg cgctagacct tttcgcccgg
     4081 ctgccggagt atacggatcg cgcgcgaggt tggcgcttcc tcattgcgat gcgcgatctg
     4141 ttcccggggc ttccgcgcct gagcgagcgg cattttgggc tggcggcgga caggccctgg
     4201 ccaagatggt cggggcggcg cttcaagtct ccccacaagg cagagcctgg gcctgcgggt
     4261 ctcgtcgcca tcttcgccga cacattcaat cgctcgttcg agcctgcgaa cttgcgcgcc
     4321 gccgcgcatg tcctcaaggc ggcaggatac ggcgttatcg cctttaccga cgagga
//
LOCUS       DATMGX010001092         2848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_59996, whole genome shotgun sequence.
ACCESSION   DATMGX010001092 DATMGX010000000
VERSION     DATMGX010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59996"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VNR65_17920"
     CDS             <1..349
                     /locus_tag="VNR65_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20599.1"
                     /translation="FDFELCLVEFRLPHEPRIGRYPYPFRRNAEEIGIKLVIPILIMD
                     GGNGIDAVFEALERNDHLTIGDYRFVLADWMPFGAVGECFPVRAFRRKHAQKNLTLVA
                     SEVVDRDCQVSGK"
     gene            665..1360
                     /locus_tag="VNR65_17925"
     CDS             665..1360
                     /locus_tag="VNR65_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20600.1"
                     /translation="MFKFVKYLLSAGLIAGSTTVAAAGDLPPEIIDSVMASCRPDYHN
                     LCSFVVPGGGRVARCLLDHEMELAPPCLKSIKLAYAMEACMPDYRRLCNGVQPGGGQI
                     VECLASRIEALAPECQRVVSANLPYAGPGHERYSYYRGPNGRPETNPEAYSYEPRPSE
                     TAPYSGGGANGGQSYDDRYADRRYDRDSGQPYGEGRYPDQGYNNPGYPQPSPGYQQPG
                     SSYPPSEEGPPLK"
     gene            complement(1458..2711)
                     /locus_tag="VNR65_17930"
     CDS             complement(1458..2711)
                     /locus_tag="VNR65_17930"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HWJ20601.1"
                     /translation="MALLADDLFQPPLVGLPEIRAAAKRLQGAILWTPAVEAPKLSAL
                     SAAHIFVKYENMQATGSFKERGALLKLLSLDDAARKRGVIAISAGNHAQAVAYHAARL
                     GIPATIVMPGHTPFNKVGNTEGYGAKVILFGETVAESRTLADRLIGENGFTLIHPYDD
                     PVVIAGQGTAGLEFIHDCADLDCIIVPIGGGGFIAGVASAVKALRPDMEIIGVEVESY
                     PSTYAALRGKIVECGGATLAEGIAVKTCGDDTLPIIRELVSDVILVSETAIEEAIFTL
                     ASVQKTVAEGASAAGLAAVLSNKERFAGRRVGLFQSGANIDSRVLASIIMRGLERESK
                     IVSLRITISDRPGTLGEISSEIGRCGANILEVYHRRFYLDVPAKGTLVDIVIETKDRA
                     HAEGTVGTLRSRGFKVERMNGVAGD"
BASE COUNT          580 a          857 c          819 g          592 t
ORIGIN      
        1 ctttgatttc gaactgtgcc tggtcgaatt tcggcttccc catgagccgc ggattggtcg
       61 atatccctac cccttccgtc ggaatgccga ggaaattggt atcaagctcg ttattcccat
      121 tctcatcatg gatggcggca acggcataga cgccgtcttt gaggcccttg aacgtaacga
      181 ccaccttacc atcggcgact accgctttgt tctggcggac tggatgccct ttggtgcagt
      241 cggggaatgc ttccctgtcc gagctttccg cagaaaacac gcacagaaga acctgaccct
      301 ggttgcttct gaggttgtcg accgtgattg tcaggtcagc ggcaagtgat tgcgaatgag
      361 aaaagcaaaa cagagttgca gcggcggccg cagtcttcaa aagaatattc atgagaatcg
      421 accgttgtaa ggaggagatg cctccgcggt aagggttcct cgcggcaaga ttgaggcaag
      481 cccaaccttt cgcgttctgc tggtatacca gatacagatt gatacaagga aggcgatagg
      541 attttgccgt cgcgcggttt agcctttgtt atggcgaaat ccagccgaga ttttgtgctt
      601 ggttggttgt cggcattcgc tcacgtagag gttttagcgg cgtgagtact gcggagatcc
      661 atcgatgttc aagttcgtga aatatcttct gagcgccggc cttattgccg gatcaacaac
      721 cgtggcggcg gcgggcgatc tgcctcctga aattatcgat agcgtcatgg cctcctgcag
      781 gcccgattat cacaatcttt gctcttttgt cgtgccggga ggcgggcgcg tcgcacgatg
      841 ccttctcgac catgaaatgg agcttgctcc accctgcctg aagtcgatca agctcgccta
      901 cgcgatggaa gcgtgcatgc ccgattatcg caggctttgc aatggcgtac agccgggcgg
      961 tggacagatt gtggaatgtc tcgccagccg gattgaggcg cttgcccccg aatgccagcg
     1021 tgtcgtgagc gcgaacctgc cttatgccgg tcctggccat gagcgttaca gctattatcg
     1081 tggccccaac gggcgccccg agacgaaccc ggaagcgtat agctacgagc cacgcccttc
     1141 tgaaaccgca ccatactcgg gtgggggcgc caacggcggg cagtcttacg atgaccgata
     1201 tgccgatcgc cgctacgacc gcgactccgg gcagccctat ggcgagggac gctacccgga
     1261 ccagggctac aacaatccag gatatcccca acctagcccc ggttaccagc agccgggaag
     1321 cagctacccg ccatcggaag aagggccgcc gctgaagtag caacgtcata tggccggggt
     1381 caagcccggc cattccaatt cagatcgact tttcggatgc gcttgggatt tcatagttcg
     1441 gcgcgtgcac tgacgcgcta atcgcctgcg acgccgttca tccgctcgac cttgaacccg
     1501 cgactccgaa gcgttcccac ggtcccttcg gcgtgcgcac gatccttggt ttcaatcacg
     1561 atatcgacaa gcgtgccctt cgccggaaca tcgaggtaaa agcggcgatg gtaaacctcc
     1621 agaatgtttg ccccgcaccg cccgatttcg ctcgatattt cgcccagcgt tcccggcctg
     1681 tcactgatcg tgatgcgcag cgacacaatc ttgctctcac gctcgagccc gcgcatgatg
     1741 atggaggcga gaacccggga gtcgatattc gcgccgcttt gaaacagacc aacgcgccgg
     1801 cccgcaaagc gctccttgtt cgacaaaacc gcggcgagcc cggcagcgct ggctccttcc
     1861 gcaacagttt tctgcacgct cgccagcgtg aagatggctt cttcgattgc ggtctcactt
     1921 acgaggatca catccgagac gagctcgcga atgatgggaa gggtgtcgtc accacatgtc
     1981 ttgacggcga tgccttccgc caaggtcgcg cctccgcatt caacgatttt tccgcgcaag
     2041 gcggcatagg ttgagggata gctttcaacc tcgacgccga tgatttccat gtccggccgg
     2101 agcgccttga ccgcagaggc aacgcccgcg atgaatccgc ctccgccgat gggaacgatg
     2161 atgcagtcga gatcggcgca atcgtgaatg aactcaagcc cggccgtgcc ctgaccggca
     2221 atcacgaccg ggtcgtcata aggatggatc agcgtaaacc cgttctcccc aatcagccgg
     2281 tcggccagcg ttcgcgattc ggccaccgtt tcgccgaaga gaataacctt cgcgccgtag
     2341 ccctccgtat tgccaacttt attgaagggc gtgtgccccg gcatgacaat cgtggcggga
     2401 ataccgagcc gtgccgcgtg ataggcgaca gcctgcgcgt gattgcccgc cgagatcgcg
     2461 atcacgcctc gtttccgcgc ggcatcgtcg agcgaaagga gcttcaggag cgcaccgcgt
     2521 tccttgaagc tgcccgtggc ctgcatgttc tcgtatttga cgaagatgtg cgcagccgaa
     2581 agcgccgaga gcttcggcgc ttccacagcg ggtgtccaga gaatggcgcc ctgcaaacgc
     2641 tttgcagccg ccctgatctc aggcaacccg accaagggcg gctggaaaag gtcgtcagcc
     2701 agtagagcca tcgcccacgc ctctccttca gcgctttata ggcaggaggg aatggatggt
     2761 tacatgccct ttgcttactg agccttcaag agtcgggccg cctcgaccgc gaaatatgtg
     2821 agaatgcccg aagctcccgc gcgacgga
//
LOCUS       DATMGX010001093         2905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_237217, whole genome shotgun sequence.
ACCESSION   DATMGX010001093 DATMGX010000000
VERSION     DATMGX010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237217"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..609
                     /gene="tnpA"
                     /locus_tag="VNR65_17935"
     CDS             181..609
                     /gene="tnpA"
                     /locus_tag="VNR65_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HWJ20602.1"
                     /translation="MQTAKSGSHTIWDCKYHIVWVTKYRYPVLVGDVGLRAREPLREI
                     SRSHEMSIHAGAINRDHVHLLLSIPPHLSVSRAVQYLKGKSSHKLLTEYQSLRKRYWG
                     QHLWSRGYWVATSGNVTDEVWKKYIEDQKPEEPDDNFKVV"
     gene            complement(737..1075)
                     /locus_tag="VNR65_17940"
     CDS             complement(737..1075)
                     /locus_tag="VNR65_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWJ20603.1"
                     /translation="MKLVVAIIKPFKLDEVRQALTAIGVHGMTVTEVKGYGRQKGHTE
                     IYRGAEYVVNFLPKLRLEVAVASDRAEAVVEALKSSAKTGQIGDGKIFVTPIDHALRI
                     RTGETDSDAL"
     gene            1318..2196
                     /locus_tag="VNR65_17945"
     CDS             1318..2196
                     /locus_tag="VNR65_17945"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HWJ20604.1"
                     /translation="MVDRSWFFASQGQQHGPYPEAQLRQFIAAGTVTAETLVWTEGMA
                     NWQKAGDVPGLLAGAPPPAMPRPGGLPMSGGYGGGSLSVDFGIWDFTWRSLVLAISFL
                     FIIPVPWALLMYCRWIVSCVHVPQRPNLGFTGRAVDLTWFYAAVLLYILAASRHSQIL
                     SLAANVVQLVLYWLLIKWFIANLTSNGQPLGLRFSGSFWGFLGWSLLAFVSVITIIGW
                     AWVYVAQLRWMCRHIEGTRRAVVFNGTGLEFLWRAIVTALLSVFIIPLPWAYRWFSRW
                     LASQTTLVDRGALASA"
     gene            complement(2203..>2905)
                     /locus_tag="VNR65_17950"
     CDS             complement(2203..>2905)
                     /locus_tag="VNR65_17950"
                     /inference="COORDINATES: protein motif:HMM:NF014595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HWJ20605.1"
                     /translation="RLPHSLHCYFILPGDPQIPIIYEVERLRDGKSYSTRRVTAIQHG
                     NAIFSIMVSFHVDEESAFDHQDKMPDVPPPEKLTAEEVAKQPIFREMPEFIRRYYESD
                     RPIELRPVELNRYFGEKIEDGRIHVWIRTASKLSDDPALHMCALAYASDFSLLDAAMA
                     RYGRTLFDKRMMPASLDHAMWFHRPFRADEWLLYAQDSPSAQGGRGLTRGMIFKQDGT
                     LVASVAQEGSLRQRK"
BASE COUNT          527 a          840 c          910 g          628 t
ORIGIN      
        1 agaagttcca gtggctgcgc gagcagcttg ccaaggatgg actcaacctg ggccttcctg
       61 ggcaataggt ttctgaaatt atccgccctg ctaataggga ggcggggatt tagagtccct
      121 tttttgggat ccgtaaaccc ctcgccttca ggcgacggga tgcggtagca tccttgtatt
      181 atgcagacgg cgaaatcggg cagtcatacg atatgggatt gcaaatatca tatcgtatgg
      241 gtgaccaaat atcgctaccc ggttctggtt ggggatgttg gactgcgagc aagggaaccg
      301 ttgcgggaaa tcagccgtag ccacgagatg tcgatccatg ccggggccat aaaccgtgac
      361 catgttcacc tgctgctctc gatcccacca cacctttcgg tgtcgcgcgc ggtgcagtac
      421 ctgaagggaa agagctccca taagcttctg acggagtatc agtcgctgag gaagcgttat
      481 tggggtcagc atctgtggtc gcgtggatat tgggtggcaa ccagtggaaa tgttacggat
      541 gaagtctgga agaagtacat cgaggatcaa aagccggaag agccggatga caatttcaag
      601 gttgtctgag aaaatcgacc agaagggtcg atcaatccgg cttccagccg taacccgaag
      661 ccaccgcctt taggcggtgg agtgttcaca tcgcttgacc ggcctcgttg ccggatgcga
      721 cggaagtgca gaaattttaa agcgcgtcgc tgtcggtctc gccggtccgg atccgcagcg
      781 cgtgatcgat cggcgtcacg aagatcttgc cgtcgccgat ctggccggtc ttggcggagg
      841 atttgagcgc ctcgaccacc gcctcggccc gatccgaggc cacggccact tccagccgca
      901 gcttgggcag gaaattcacg acatattcgg cgccgcggta gatttcggta tggcccttct
      961 ggcgcccgta acccttgacc tcggtgaccg tcatgccgtg gacgccgatg gcggtcagcg
     1021 cctggcgcac ctcgtccaac ttgaagggtt tgatgatggc aacaacgagc ttcatggtcg
     1081 ggctttcatt tctgtcgaat tccggtggcc gcctggccgt ccaagtccaa ctccaagtct
     1141 ggcagctttt tgtgcaaatg acacaaaaag ttgcaatgag aaaccgaaat cgattgccgt
     1201 ttttcacggc ggttcgctag gcttgccggg gggagcaggg aattttccaa gacaattcga
     1261 ttttttgcca gcgctcctgc cggcgccgcc cacaacagcg cccctgaggg gagtaaaatg
     1321 gtcgaccgat cctggttctt tgcgtcccaa ggccagcagc acgggcctta ccccgaagcg
     1381 caactccgcc agttcattgc cgcggggacg gttaccgcgg agacgctggt gtggaccgaa
     1441 ggcatggcga attggcaaaa ggccggggat gttcccggcc tcttagcggg cgcccctccc
     1501 ccggccatgc cacggcccgg cggcctgccc atgagcggcg gctatggtgg cggctcgctg
     1561 tcggtcgatt tcggaatctg ggatttcacc tggcgcagcc tggtgctggc catcagcttt
     1621 ctgttcatta tcccggtgcc gtgggcgctg ctgatgtatt gccgctggat cgtttcctgc
     1681 gtgcacgttc cgcagcggcc aaatctcggt ttcaccggcc gcgcggtcga cctgacgtgg
     1741 ttctatgcgg ccgtgttgct ctacatcctc gcggcatcga gacattccca aattctcagc
     1801 cttgcggcca acgtcgtgca gctggttttg tactggctcc tgatcaaatg gttcattgcg
     1861 aaccttactt cgaacggcca accactcggg ttgcgcttct cgggctcgtt ctggggcttc
     1921 cttggctgga gcctgctggc cttcgtttcc gtcatcacca tcatcggctg ggcgtgggtc
     1981 tacgtggcgc aattgcgctg gatgtgccgt cacatcgagg gcacgcggcg cgccgtcgtt
     2041 ttcaatggca ccgggctcga gtttctttgg cgcgcgattg tgacagcgct gctcagcgtc
     2101 ttcatcatcc ccctgccgtg ggcgtaccgc tggttcagcc gatggctcgc gtcgcagacg
     2161 acgctggtgg acagaggcgc gctcgccagc gcgtaaaacg ctttacttgc gctggcgcag
     2221 cgagccttcc tgcgccaccg aagccaccag cgtgccgtcc tgcttgaaga tcatgccgcg
     2281 cgtcaggccg cggccgccct gcgcgctcgg cgaatcctgc gcgtagagca gccattcgtc
     2341 ggcgcggaac ggccggtgaa accacatcgc atggtcgagg ctcgccggca tcatgcgctt
     2401 gtcgaacaga gtgcggccgt aacgggccat cgcggcatcc agcagcgaga aatccgaggc
     2461 ataggccagc gcacacatgt gcagcgccgg atcgtcggac agttttgagg cggtgcggat
     2521 ccagacatgg atgcggccgt cctcgatctt ttcgccgaaa tagcggttga gctcgaccgg
     2581 gcgcagctcg atcgggcggt cggattcgta atagcggcgg atgaattcgg gcatctcgcg
     2641 gaagatcggc tgctttgcaa cttcctcggc ggtgagtttt tccggcggcg ggacgtcggg
     2701 catcttgtcc tggtggtcga acgcgctttc ttcgtcgaca tgaaacgaca ccatgatcga
     2761 gaagatggcg ttgccgtgct ggatcgccgt gacccgtcgg gtcgaatagc tcttgccgtc
     2821 gcgcaagcgt tcgacctcgt agatgatcgg tatctgcggg tcgccgggca ggatgaaata
     2881 gcagtgcagc gaatgcggca ggcgg
//
LOCUS       DATMGX010001094         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_189333, whole genome shotgun sequence.
ACCESSION   DATMGX010001094 DATMGX010000000
VERSION     DATMGX010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189333"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VNR65_17955"
     CDS             complement(<1..811)
                     /locus_tag="VNR65_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20606.1"
                     /translation="MGWTRKNLVIGNDLQSRRFVLDPKGDLEEMPFFRVAEGRPVFHK
                     PFSLSLDLVDGRVGIFVSNDPKAFLLTRDERSQALRIPVFFVGHPRESLKELEDNANG
                     LVFDPSALVDAGSDFSQVTFHLKRGGSRFDVIRPFFTCGLAGLLVCEESCPEDSDILA
                     ASLVNLIVRGESGTPSSSGREEANAPAARESRRPRAGPPVALPPLKPKPAERFTKAPS
                     SPPEELEPAPLPPPRVRSSENAAPSAKTENCPACPPPAPRTEIPAQPQKPEA"
     gene            1232..1429
                     /locus_tag="VNR65_17960"
     CDS             1232..1429
                     /locus_tag="VNR65_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20607.1"
                     /translation="MKSDPAKFVLSASENTATLTVLATSLGETGTLDLAIRFPVRRFA
                     KLAVPAEAAVRRVQNGNYHHS"
     gene            complement(1521..>2537)
                     /locus_tag="VNR65_17965"
     CDS             complement(1521..>2537)
                     /locus_tag="VNR65_17965"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HWJ20608.1"
                     /translation="PRVGAFNAKRKTPAQLERDIREVLEGRGEKLGYFSVDVSRCRPF
                     YILGLVDHPGSYPFSPGFTVLHAISTAGGLYRQPAGERLSIADTLSRRGALLARRARL
                     QAEKDGSPTIPLPKELVQLESSDATDMIAREQSILEQSRQVSERERNTLETIVGINHS
                     EVENYNKEIVKLEQRIAEQMQTYTQLKKLHEDKVINQQRFFESVAALDNLQRDRRSTG
                     SNLSQATSNLERSRRDLAILKLAESARIVKEIGETEIELERLKKAAAQSGDLAYGMDA
                     ASAKSNGGSIATYKIVRRNEAGRPEVLRAYETTPIMPGDIIEVGSSKLSDKVLESKES
                     NQNY"
BASE COUNT          522 a          725 c          718 g          572 t
ORIGIN      
        1 cggcttccgg tttttgtggc tgagccggaa tttccgtgcg tggcgctggc ggagggcaag
       61 cggggcagtt ttccgttttc gcagacggag ctgcattttc ggaagatcgc acccgtggag
      121 gaggtaaggg cgctggttcc aattcttcgg gcggacttga gggcgctttc gtgaatcgct
      181 cagctggctt gggctttaaa ggcggcagcg cgacaggcgg gcctgcccgg ggccggcggg
      241 actccctcgc ggcgggcgca ttggcttcct cgcgtcctga cgacgacgga gttcccgact
      301 cgcctctgac aatcaggttg accaatgaag cagccaaaat gtccgaatct tcggggcagg
      361 actcttcgca caccagcagg cccgccaatc cacacgtgaa aaatgggcgg atcacatcga
      421 agcgggaccc ccctcgcttg agatgaaagg tcacctgcga gaaatcgctc cctgcgtcca
      481 ccaatgcaga cgggtcgaag acaaggccat tggcgttgtc ctctagctct ttgagggatt
      541 cgcgcgggtg gcctacgaag aagaccggta ttcttagtgc ttgggatctc tcgtcccgtg
      601 taagcagaaa cgcttttggg tcgttactga cgaatatccc cactctgccg tcaacaagat
      661 cgagcgacag gctgaatggt ttgtggaaga cagggcggcc ctccgccacc ctgaaaaacg
      721 gcatctcctc caggtcgcct ttggggtcca gcacgaagcg gcgggattgg agatcgttgc
      781 caatcacgag gtttttccgg gtccaaccca cgcaggcctc tcccgacccg ccacgcgcag
      841 ccgtgggctg cggcgcaaat agtgtcacgc aggctaggag agccagcgag caaaaaccgc
      901 agcggagacc gatttgcgta ggcgatgtca tagggcagga gtgaatcccg agtcgaacgg
      961 cggcaattta actctaattg accttaaccg caaaacgcct agcgcagcat cgttaatcat
     1021 tcgaatgcgt gccgcaggct gtacaacaaa tagttcgtac cgggtctcga atcctatgtc
     1081 ccgcgaaact tcatgcccag cgtgaagggg ggctcgaatc tctcgaacgg atcgcagcaa
     1141 cgctcaacgg aataccaaag ttcacctttc cagcgaaggt agagtcgagt ggcggatcaa
     1201 gagatatcgc agcgtagctg tcgagagccc ggtgaaatcc gatcctgcta aatttgtgtt
     1261 gtctgccagc gaaaacaccg cgacgctgac agttctcgcg accagcttgg gcgaaaccgg
     1321 gaccctcgat ctggcgatca ggtttccggt ccgccggttt gccaagcttg ccgttccggc
     1381 ggaggctgct gttagaagag tacaaaatgg caactatcac cattcctaac ccacccggag
     1441 ccgccatcca cccttcgacg ccaacccttg ctgcgagatt accagataga gaatgcaaca
     1501 tgctttccca ccggatgcag tcagtagttc tggttggact ccttactttc taacacctta
     1561 tcggagagtt tactcgatcc aacctcaatg atgtctccag gcataatggg agtcgtctca
     1621 taggctctta gaacctcggg acgcccggcc tcattgcgcc ttacgatttt ataggtagcg
     1681 attgatcctc cgttgctttt ggccgaggct gcatccatgc cgtacgcgag gtcgcccgac
     1741 tgtgcggcgg ctttcttcag cctttcgagc tcgatctcgg tttcaccaat ttctttgacg
     1801 atgcgcgcgc tctccgcgag cttcaggata gcgaggtctc gtcgggatct ctctagattg
     1861 ctagtcgcct gcgagaggtt agaacctgtc gaccgcctgt cgcgttgcaa gttgtccaaa
     1921 gccgcgaccg attcgaagaa acgctgctga ttgatgacct tgtcctcatg cagcttcttc
     1981 agctgcgtgt aggtctgcat ctgctcggcg atcctttgtt caagcttaac gatctcctta
     2041 ttgtagtttt ccacttcgct gtgattgatt ccaacgatgg tctcaagtgt atttctttca
     2101 cgctcgctaa cctgccggga ctgttccagt atggattgtt ctctcgctat catgtccgtg
     2161 gcatcagacg actctaactg aacgagttct ttgggcagcg ggatagtggg agagccgtcc
     2221 ttttcggcct gaaggcgagc tcgcctggca agcagcgctc cgcgacgact taatgtgtca
     2281 gctatcgata gcctttctcc ggcgggctgc cgataaaggc cgcctgctgt cgatattgcg
     2341 tgcaagactg tgaaccccgg cgagaaggga taggacccgg gatgatcgac aaggcctaag
     2401 atgtagaagg gccggcagcg actgacatcg acggagaagt agcctagctt ttcgccccta
     2461 ccctccagaa cctcgcgtat gtcacgctca agctgtgcag gagtcttcct cttcgcattg
     2521 aatgcgccta cccgtgg
//
LOCUS       DATMGX010001095         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_192825, whole genome shotgun sequence.
ACCESSION   DATMGX010001095 DATMGX010000000
VERSION     DATMGX010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192825"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1822)
                     /locus_tag="VNR65_17970"
     CDS             complement(176..1822)
                     /locus_tag="VNR65_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012167412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HWJ20609.1"
                     /translation="MSLQLHPIGPIPEETERVARSAFPKSNIYLRLRDELGAIYDDQT
                     FASLYPSRGQSAFSPWRLAMITVMQFAENLSDRQAADAVRGRIDWKYLLGLPLTDSGF
                     DHTVLSEFRCRLIADKSEGLLLDTLLDRLRSLELIKPRGRQRTDSTHVLAAVRRLNRL
                     ERVGETLRAVLNDIAVEAPQWLQALATAEWYDRYSGRVENYRLPKTDAAREQLASTIG
                     GDGRKLLLAIDTMGIPELAKLPAVRMLRQVWAEQYIEENGQIRWREVKEMPAPASLIS
                     SPYDSEARYSKKRETTWVGYKVHLTETCDPETPHLITNTETTLATVPDDNMLKTVHQS
                     LKDHDLLPAEHLVDKGYTDARVLADNPRDYGVTIVGPVADDPSWQARSGEGFDKSAFA
                     VDWDRQIVTCPGDRQSISWLPNTYPKNGAVFEARFSAKDCTPCSLRPKCTKAKREPRI
                     IGLQSRQHHETLQTARQHQTTEDFKQSYAARAGIEGTHEQAIRRCGLRRCRYIGIAKT
                     RLQHVATAVAINLVRMDAWWTSTPRAKTRCSHFEALRRAA"
BASE COUNT          447 a          607 c          575 g          478 t
ORIGIN      
        1 gcgtctcatc gtagagggaa cctcagtgcc agacgcttca aggattctca aagtccatcg
       61 cactacgcta tatcgagctt tggagccata aagaccggac gttcctaccc cgcgggtgag
      121 ccaaatggcc cctggtgaca cgactgttga tactggtggc gaattcgcaa tcaccctagg
      181 cagccctccg caaggcctcg aagtgggagc atcgcgtttt tgctcttgga gtgctggtcc
      241 accacgcgtc catccggacc aaattgattg cgacggcggt ggcgacatgc tgcaggcgtg
      301 tcttcgcgat cccgatatag cggcatctcc gcaatccgca gcgacggatc gcctgttcat
      361 gtgttccctc aatgcctgcg cgtgcggcgt agctttgttt gaaatcttcc gtcgtttgat
      421 gctgtcgggc ggtttgcagc gtctcatgat gctgacgcga ttgcagtccg atgatgcggg
      481 gctctcgctt ggctttggtg cacttgggcc gcaacgaaca aggcgtacaa tccttggcgg
      541 aaaatcttgc ctcgaacaca gcgccgttct tcggataggt gtttggtagc cacgagatac
      601 tttgcctatc ccccgggcag gtgacaattt gccggtccca atcgactgcg aacgctgatt
      661 tgtcaaaacc ctcgccagat cgcgcctgcc agctggggtc gtcggccacc ggcccgacaa
      721 tggtaacccc gtaatcacgg ggattgtcgg cgagcacgcg agcatccgtg tatcccttgt
      781 cgacgaggtg ctcggcaggt agcaggtcgt ggtctttaag tgactgatgg acagtcttga
      841 gcatgttgtc gtcaggaact gtcgccaagg tcgtttctgt attcgtgatt aaatgcggtg
      901 tttccggatc gcaggtctcc gtgagatgca ccttgtagcc gacccaagtg gtttcacgct
      961 tcttgctata tcgcgcctca gaatcatatg gggatgaaat cagcgaagct ggcgcaggca
     1021 tctctttgac ctcgcgccat cttatttgac cattttcctc aatatattgt tcggcccaga
     1081 cctgcctcag catccgtacc gctgggagtt tcgccaattc cggaattccc atcgtatcaa
     1141 tggcgagcaa cagctttcgc ccatcgccgc cgatcgtgct cgccaactgc tctcgcgcgg
     1201 cgtcggtctt cggcaaacgg tagttttcga cacgaccgct gtaacggtcg taccattcag
     1261 ccgtcgccaa cgcctgtagc cattggggcg cttcgaccgc tatgtcgttc agcacggcac
     1321 gcagggtctc gccgacacgt tcaagccgat tgaggcggcg caccgcagca agcacatggg
     1381 tcgagtccgt ccgctgccgc ccccgtggct ttatcaattc aaggcttcgc aagcgatcaa
     1441 gtaaagtgtc aagcagaagt ccctcagact tgtccgcaat aagacgacag cgaaattcac
     1501 tgagcacagt atgatcaaag ccggaatcgg tcagcggtaa gccgagcaaa tatttccagt
     1561 cgatacggcc tcgaaccgca tcagccgcct ggcggtcgga caggttctcc gcaaactgca
     1621 tcacggtgat cattgccaag cgccacgggg agaaagccga ttgtccgcgg ctgggataaa
     1681 gcgatgcgaa agtttgatcg tcatagattg cgcccaactc gtcccgtaac cgaagataaa
     1741 tgttgctctt cggaaaagct gaacgagcga cgcgctccgt ttcctcagga atcgggccga
     1801 ttgggtgcaa ttgaagtgac atgtccatcc atcgattgaa gaatcagatg gaaagacaaa
     1861 ttatcgatca agaatcgccg agactcgcgc aaagacatcc tcatccgtga gtcaaagtga
     1921 cgcagcgcgc gcgacttgcg cccgtcccct aattcgccac cagtatcaat aagcgtgtta
     1981 tcctggttca gggcggtgaa agatcggctg agatcgttcg attgtagcat ggggcgctcc
     2041 attggtttct tagacagcgc cgaaagtgcc acaccttcgc cgctcgcccc ataccatctt
     2101 catcaag
//
LOCUS       DATMGX010001096         3124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_211532, whole genome shotgun sequence.
ACCESSION   DATMGX010001096 DATMGX010000000
VERSION     DATMGX010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211532"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1788)
                     /locus_tag="VNR65_17975"
     CDS             complement(205..1788)
                     /locus_tag="VNR65_17975"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWJ20610.1"
                     /translation="MNKSDAPQIISLSAAELEKLLGELRGPLAPATYQMVEALLRTLQ
                     WVMATLEQKTATLGRLRRMIFGEKTEKACKILPATLAAILSGPAKPKAKGHGRKGAKD
                     YPAAQRVQVPHPKHKAGDLCPKCLKAKLYRFLTPARLVRIVAQPIFQATVFELERLRC
                     ALCGALFTAPAPPEAGLGKYDPSVGTMLALLRYGAGLPMYRTGKWQNHFGVPLPASTQ
                     WELIEAASETPQLIYEALIGVAAQGHLLHNDDTHMRIQSVRQEIAAAGSEQERTGIFT
                     TSIISKTGQRQIALFFTGQKHAGENLNQLLQRRAAGLDQPMQMCDALSRNEPKEFHTL
                     LCHCLLHGRRQFVDVVENFPEECRKVIESLREVYRFDALTQEQKLSDLDRLAFHQAHS
                     QPVMNDLQEWMQEQIEQKKVEPNSGLGEAIQYMLKRWQTLTRFLSVPGAPLDNNIAER
                     ALKMAILHRKNSLSFKTLHGARIGDVHMSLIHTCELNRVNPFDYLMALQQHAAAVSKA
                     PESWLPWNFQQAIAAAGSG"
     gene            complement(1781..2668)
                     /locus_tag="VNR65_17980"
     CDS             complement(1781..2668)
                     /locus_tag="VNR65_17980"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HWJ20611.1"
                     /translation="MQTEFRYRGRNISGAEIESVRQLIADHPGLSRRRLSAKLCQAWN
                     WVQANGQLRDMVARSLMLQLHRAGLIALPAKRKSVPNNAVRHRTPLLEPPLEVAPLEC
                     SLAELGPLEIRQVRRTPEEALFGRLLQTYHYLGYRRPVGEHVKYLVYGRGQPIACLAW
                     SSAPRHLGPRDRFIGWSASQRQAQIHLLAYNTRFLILPWARVPHLASHLLARAARRIS
                     ADWQQLYQHPIYLLETFIEPARFRGTCYRAANWLFLGLTTGRGKDDLTHQPNRSLKEL
                     WVQPLGRDFRQRLCREGHE"
     gene            complement(2821..>3124)
                     /gene="tnpB"
                     /locus_tag="VNR65_17985"
     CDS             complement(2821..>3124)
                     /gene="tnpB"
                     /locus_tag="VNR65_17985"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HWJ20612.1"
                     /translation="KGIDGLAQVCRQQLKIDPMAGALVVFSSRRRKALKCLVFDGQGF
                     WLCQKRLSTGRFAWWPTEAKPPTFGLDAHQLQSLLWNAQPASAQAVPLWRPITPVG"
BASE COUNT          614 a          929 c          959 g          622 t
ORIGIN      
        1 attgaaaccg aggaattgaa caatacgttt ccggtgttcg tcggtcgggc ggttctggtt
       61 cttttcccat ttctcgattg cagagtcgct gattcgtgtt gctccggtca gcttgcgtaa
      121 cggcaaaaag ccgcaccgcg gcgggttttc ggcggttccg cgtggccggg cgcaggaaac
      181 tgggtgggag cggaaactga taagtcaacc gctgccagcc gccgcgatgg cctgctgaaa
      241 attccaaggc agccaactct ctggggcttt ggacacggcg gcagcgtgtt gctggagggc
      301 catcagataa tcaaacggat tgacccgatt gagttcacag gtgtggatga ggctcatgtg
      361 aacgtcgccg attctggccc catgcagtgt tttaaagctc agggagtttt tccgatggag
      421 gatcgccatc ttgagagcgc gttccgcaat gttgttatcc aggggcgcgc ccggcaccga
      481 aaggaaccgg gttaaggtct gccaccgctt gagcatgtat tggatggcct ctcccagacc
      541 cgagttgggt tcgaccttct tttgctcgat ttgctcctgc atccattctt ggagatcgtt
      601 catcaccggc tggctgtgcg cctggtgaaa ggccaggcga tccaggtccg aaagtttttg
      661 ctcctgggtg agggcatcga accgatacac ctcgcgcagg ctttcaatga ccttgcgaca
      721 ttcctccggg aagttctcga ccacgtccac gaactggcgc cgtccgtgca gcagacagtg
      781 acacagcagc gtgtggaact ccttgggttc gttgcgcgag agcgcatcgc acatctgcat
      841 gggctggtcc aacccggccg cgcggcgctg gagcaattgg ttgagattct ccccggcatg
      901 cttctggccg gtgaagaata gagcaatctg gcgttggccc gtcttggaga tgatgctggt
      961 ggtgaagatg ccggtgcgct cctgttcgct gccagccgcg gcgatttcct ggcgcacact
     1021 ttggatgcgc atgtgggtgt catcgttgtg cagcaggtgg ccttgggcgg cgacgccgat
     1081 cagggcctcg tagatgagct ggggtgtttc cgaggcggcc tcgatcagtt cccattgcgt
     1141 tgaggccggc aaaggaaccc cgaaatgatt ttgccatttg ccggtccgat acatcggcag
     1201 accggccccg tagcgcagca gagccagcat ggtgccgaca ctcgggtcgt atttacccag
     1261 tcccgcctct gggggagcgg gagcggtaaa gagagccccg cacagagcgc aacgaagccg
     1321 ttccaattca aagaccgtcg cctggaagat gggctgggcc acaatgcgaa ccagccgggc
     1381 tggggtcaag aaccggtaga gtttggcctt gaggcacttg ggacacaggt ctccggcttt
     1441 gtgcttagga tgaggcacct gaactcgctg ggcggccgga taatccttgg cccccttgcg
     1501 cccgtgtcct ttggccttgg gcttggctgg cccggagagg atcgccgcaa gggtcgcggg
     1561 aaggatcttg caagccttct cggttttctc tccgaaaatc atccgccgga gccggcccag
     1621 ggtggccgtc ttctgttcca gcgtcgccat gacccattgc aaggttcgca gcaaggcttc
     1681 caccatttga tacgtcgccg gtgccagtgg cccgcgcagt tcgcccagga gtttttccaa
     1741 ctccgccgcg ctcaggctaa taatctgtgg ggcgtcgctt ttattcatgg ccttcccggc
     1801 aaagccgctg acgaaagtcc cgccccagag gctggaccca aagttccttg agcgagcggt
     1861 tgggttggtg ggtgagatcg tccttgcccc ggcccgtggt caagcccagg aaaagccagt
     1921 tggccgcgcg atagcaagtg ccgcgaaagc gggccggctc gatgaaagtt tccagcaaat
     1981 aaataggatg ctggtagagt tgctgccaat cggccgagat tcggcgcgcc gctcgagcca
     2041 acaaatgact ggccaagtgt ggaactcgcg cccagggcaa aatcaaaaat cgcgtgttgt
     2101 aagccagcag gtggatctga gcttgccgct gcgatgcaga ccagccgatg aaacggtctc
     2161 gcggacccag gtgccgcggc gccgacgacc acgccagaca ggcaatcggc tggccccggc
     2221 catagaccag atatttgaca tgctccccga ctggccgacg ataccccaga tagtgatagg
     2281 tttggagcaa ccggccaaag agggcttcct ccggcgtgcg gcggacttgc cgaatctcca
     2341 acggccccaa ctcggccaaa ctgcattcca acggcgcgac ctccaagggc ggctccagca
     2401 acggtgtccg atgccgcacc gcgttgttgg gcacagactt tcgcttggcc ggcagtgcaa
     2461 tcagcccggc ccggtgcaac tgcaacatga gactgcgggc caccatgtct cggagttgac
     2521 cgttggcttg cacccaattc caagcttggc agagcttggc ggaaagccgc cgccgactca
     2581 gcccgggatg atcggcgatc aactgccgga cggactcaat ttcggcgcca ctgatattgc
     2641 gtccgcgata acgaaactcc gtttgcacga ccgccagcat acgcggtcga ttttggaacg
     2701 caagcccctg cttgaacctt gcttgctgcc ctaaacactc tcacccaaaa cgctccggtc
     2761 taatcaatct gccgcaagca tccagtaacg gaggcaatgg aggcagagag ccccgaggcg
     2821 tcaacctaca ggcgtgatag gccgccacaa cggcaccgcc tgggcgctgg ccggctgggc
     2881 attccaaagc agcgactgga gttgatgggc gtccagcccg aaggtcgggg gtttcgcctc
     2941 ggtcggccac caggcaaagc gcccggtgga aaggcgcttt tgacacagcc aaaaaccttg
     3001 gccatcgaaa acgaggcact tcaacgcctt gcgccgccga ctcgaaaaaa ccaccaaagc
     3061 acccgccatc gggtcgattt tgagctgttg ccggcacact tgcgccaagc cgtctatccc
     3121 cttg
//
LOCUS       DATMGX010001097         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_187653, whole genome shotgun sequence.
ACCESSION   DATMGX010001097 DATMGX010000000
VERSION     DATMGX010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187653"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VNR65_17990"
     CDS             complement(<1..389)
                     /locus_tag="VNR65_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20613.1"
                     /translation="MNSKGIVGLVICCVFAWPQSGSAAADAPLPTDEAAILARIRDAA
                     MSSDWAWQRLGDLTDKIGPRLSGSVQLTAAINQLADTMRSLPAQVTLQPVKVPHWVRG
                     EEQAELVDYPGRPVGVTQRLHLTALGSS"
     gene            complement(599..1303)
                     /locus_tag="VNR65_17995"
     CDS             complement(599..1303)
                     /locus_tag="VNR65_17995"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HWJ20614.1"
                     /translation="MNLNKSARRLAGLLCIAVSLGLACSSALRAADSPPEMTADGLRL
                     QKNTGTRLVYVRPGATFGKYDKVAILDCLVEFDKNWQNNYNSQQVDPSAFVSTDDMNR
                     IKKELAAEFKKVFTRELQANGGYPVVDSAAPDVLVLRPAIINLRVTQPDLMTPGIDMT
                     VIRSAGSMTLYLELWDSVTNTILARVMDAQTDQGLGGPQVANSVTNTMAADFILRQWA
                     TALRKHLQAARASSTG"
     gene            complement(1360..2112)
                     /locus_tag="VNR65_18000"
     CDS             complement(1360..2112)
                     /locus_tag="VNR65_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HWJ20615.1"
                     /translation="MSLSLPVIVIMLLLLTASSIRILKEYERGVMFTLGRFTGVKGPG
                     VRLVLPGIQQMKKVELRTVVLNVPKQDVISRDNVSVKVSAVVYFRIVDPEKAILQVTN
                     PWEATSQLAQTTLRSVAGQHEMDEMLAQRDKLNAAIQQVLDANTAAWGMKVSNVEIKD
                     IDLDETMIRAMARQAEAERARRAKVIHAEGEKQAAQSLAEAAKLLSSEPASLQLRYLQ
                     TLADISSEKSSTIVFPLPIDLIAPLLRSLKHE"
     gene            complement(2125..>2305)
                     /locus_tag="VNR65_18005"
     CDS             complement(2125..>2305)
                     /locus_tag="VNR65_18005"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWJ20616.1"
                     /translation="VETMIGATVEAVSDCEHRCVVRYGGELWNARSASPLRAGQQARI
                     VKVEDLTLWVEPQQN"
BASE COUNT          427 a          740 c          673 g          465 t
ORIGIN      
        1 gaggaaccaa gggccgtcaa atgcagacgc tgcgtcacgc cgaccggtcg ccccggatag
       61 tcgaccaatt ccgcctgctc ttcgccgcgc acccagtgcg gaactttcac cggctgcaac
      121 gtcacctgcg cgggaagcga gcgcatcgtg tccgccaatt gatttatcgc cgcggtgagc
      181 tgcacggaac cggacaagcg cggcccgatt ttgtcggtga gatcgcccag acgctgccag
      241 gcccaatcgc tcgacatggc ggcatcgcga atccgcgcaa ggattgcggc ctcgtctgtc
      301 gggagtggcg cgtccgcggc ggcactgcct gattgtggcc acgcaaagac acagcagatt
      361 acgagaccaa caataccctt ggaattcatg actcattccc atccgtgcaa acccgagcgc
      421 aactgcgcgg ccggccaagc gtgtcatttg cggcagtcat tcggcaaacc ggcgttcgca
      481 cgggcgctca ggcgcgagac ggctggtgcc ggatcttccc cgcttctcgg gggtcgcgca
      541 aatcagcttc tcgatggccc acggtgtgag agatcgcctc cgtcagcggc ttgcgcactc
      601 agccggtcga actggcgcga gcagcctgca gatgcttgcg cagcgcggtg gcccattgcc
      661 tgagaatgaa atccgccgcc atggtgttgg tcacactgtt ggcgacctgg ggcccgccca
      721 aaccctgatc ggtctgcgca tccatgacgc gggccaggat cgtgttggtc accgaatccc
      781 aaagctccag gtacagcgtc atcgagccag cggagcggat cacggtcatg tcgattcccg
      841 gcgtcatcag gtccggctga gtcacccgga ggttgatgat ggccggccgc agcaccagca
      901 cgtccggggc ggcgctgtcg accaccggat agccgccgtt cgcctgcagc tcccgcgtga
      961 aaaccttctt gaattccgcg gccagctcct tcttgatccg gttcatgtcg tccgtgctga
     1021 cgaaagcgga ggggtccacc tgttgggagt tgtagttgtt ctgccagttt ttgtcgaact
     1081 ccaccaagca atcgaggatc gcgaccttgt cgtacttgcc gaacgtcgcg cccggcctca
     1141 cgtagaccag gcgagtgccc gtgttcttct gcaggcgcag gccgtccgca gtcatctcgg
     1201 gcggcgagtc cgcggcgcga agcgccgatg agcaggccaa gcccaaactg accgcaatgc
     1261 aaagcaaccc cgccaaccgt cgtgccgatt tgttcaggtt cataaaaatc tctattgagg
     1321 actccgcgca acggtatcgt agcgcaaatg ccggtcccgt tattcgtgtt tcaaggatcg
     1381 cagcagcggc gcgatcagat cgatgggcag cgggaataca atggtcgaac tcttttcgct
     1441 ggagatgtcg gcaagcgtct gcagatagcg cagttgcaac gaagcgggtt cgctcgacag
     1501 gagcttggcg gcttccgcca gactttgagc ggcttgtttc tcgccttccg cgtggatcac
     1561 cttggctcgc ctggcgcgct cggcctcggc ttgtcgagcc atcgcgcgga tcatggtctc
     1621 atcgagatcg atatccttga tctcgacatt gctcaccttc atgccccagg ccgccgtatt
     1681 ggcgtccagg acctgctgaa tggcggcatt gagcttatcg cgctgagcca gcatctcgtc
     1741 catttcatgt tgtcccgcca ccgatcgcag cgtcgtttgc gcgagctgac tggtcgcctc
     1801 ccaggggttg gtgacctgca gaatggcctt ttccggatcg acgatgcgaa aatagacgac
     1861 ggcgctgacc ttcaccgaca cattgtccct ggatatcaca tcctgcttcg gcacattcag
     1921 caccacagtg cgaagctcaa ctttcttcat ctgctgaatc cccggcagca ccagccgcac
     1981 gccggggcct ttgacgccgg tgaaccggcc gagcgtgaac atcacgccgc gctcgtattc
     2041 cttcaagatg cgtatcgaac tcgcggtcag cagcagcagc atgatgacga tgacgggtaa
     2101 tgaaaggctc atcggtgact ccttctagtt ctgttgcggt tccacccaaa gcgtcagatc
     2161 ttccactttg acaatgcggg cctgttgtcc cgcacgcagt ggacttgcgc tgcgggcgtt
     2221 ccacaattct ccgccatagc gcaccacgca ccgatgctcg caatcgctga ccgcttcaac
     2281 ggtcgcgccg atcatcgtct cgaca
//
LOCUS       DATMGX010001098         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_13386, whole genome shotgun sequence.
ACCESSION   DATMGX010001098 DATMGX010000000
VERSION     DATMGX010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13386"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VNR65_18010"
     CDS             <1..763
                     /locus_tag="VNR65_18010"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWJ20617.1"
                     /translation="KFYSTFPYVPLSSISYDQRKPVPETTNWAGNVYHGLPKDSLSFS
                     ARPKGGYLAFLGRISPEKRPDRAIEIAARAGLPLKIAAKVDKADQAYWDEKIEPMIRA
                     TPNVEYIGEIGENQKARFLGDALALVFPIDWPEPFGLVMIESMACGTPVIAFECGSVP
                     EVIDHGVSGFVVKSVEEALAAVSRIPDLDRAEVRQAFQNRFTAGRMARDYLKMYASLP
                     GVRKHGEARTMNGAEISFRPADVGNPAWRRPVLQP"
     gene            complement(836..1312)
                     /gene="rlmH"
                     /locus_tag="VNR65_18015"
     CDS             complement(836..1312)
                     /gene="rlmH"
                     /locus_tag="VNR65_18015"
                     /inference="COORDINATES: protein motif:HMM:NF000989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HWJ20618.1"
                     /translation="MKISILAVGRLKSGAEQTQFNRYFDLFNAAARGLKMPPMAVVEI
                     SEGRNHSAAERKDAEAQELLNKVGGARLVVLDEDGKSLTSYEFTAMISRLRDEALDVA
                     FAIGGPDGHGDTLKKAAHLQLSLGRMTLPHGLVRIVLAEQLYRAMTIIQGHPYHRA"
     gene            complement(1341..1754)
                     /gene="rsfS"
                     /locus_tag="VNR65_18020"
     CDS             complement(1341..1754)
                     /gene="rsfS"
                     /locus_tag="VNR65_18020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWJ20619.1"
                     /translation="MRPQALNHEFAELKPRRAKRAPAASASLAHLIIDALEDAKADNI
                     VSIDLAGKTSLGDTMIVASGRSQRHVGAIAERLIERLKEEGVRDTRVEGMPLCDWVLI
                     DAGDVIVHVFRPEAREFYNLEKMWGSDRPKREAAD"
     gene            complement(1946..>2458)
                     /locus_tag="VNR65_18025"
     CDS             complement(1946..>2458)
                     /locus_tag="VNR65_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20620.1"
                     /translation="IFMVMIVSKRAGAVLAAAGLCVAVSLPALSQGFGLRIDGDRGSI
                     EVTPPQDERGNGEDQRRHEERGDAEGHRTRGFGFHFNDDERGNVEGKRAACETYARVA
                     LVQAEANRKYRCGYTGPRWEIEVEPHFHWCRHVRRESLGMEARERAGELQRCFDRLGD
                     FDDEGFEHRR"
BASE COUNT          465 a          731 c          736 g          526 t
ORIGIN      
        1 caagttttac tccacgttcc cctatgtgcc attgtcttca atttcatacg atcagcgcaa
       61 gccagtccct gaaacaacga attgggcagg caatgtctat cacgggcttc ccaaggacag
      121 cttgagcttt tcggcccggc cgaagggcgg atatctcgcg ttcctcggcc gtatatcgcc
      181 ggagaaaagg cccgaccgcg ccattgaaat cgcggcgcgc gccgggcttc cgctgaagat
      241 cgcggcgaag gtcgacaagg ccgatcaagc ctattgggac gagaagatcg aacccatgat
      301 cagggccacc ccgaacgtcg aatatatcgg cgaaatcggc gagaaccaga aggcacgctt
      361 tctcggcgac gcactggcgc tggtgtttcc tatcgactgg cccgagcctt tcggtctcgt
      421 catgatcgaa tccatggcct gtggaacgcc tgtgatcgcg tttgaatgcg ggtctgttcc
      481 tgaggtaatc gaccacgggg tcagcggttt tgtggtaaag agcgtcgagg aggcccttgc
      541 cgccgtgtcg cgtatccccg acctcgaccg cgccgaggta cgccaggctt ttcaaaaccg
      601 gttcacggcc ggccgcatgg cgcgcgatta tttgaaaatg tatgcgtcgc tgccgggcgt
      661 ccggaagcat ggcgaggccc ggaccatgaa tggcgccgaa atctccttca ggcctgccga
      721 tgtaggaaac ccggcgtgga gacgaccggt actgcagcca tagcactttg gaattgatca
      781 acttttctgc ctgtaatcga ttccgactaa ggcaggttaa tccggtcccg aggcctcacg
      841 cgcggtggta ggggtgaccc tggatgatgg tcatggcgcg gtagagctgt tcggcaagca
      901 caatgcggac gaggccgtgg ggcagggtca tgcgcccgag agagagctgg aggtgcgcag
      961 ctttcttgag tgtgtcgccg tgtccgtccg gcccgccgat ggcgaaagcg acatcgagcg
     1021 cttcgtcgcg aagccgcgag atcatcgcgg taaactcata ggatgtcagc gacttgccgt
     1081 cctcgtcgag cacgacaaga cgggcaccac ccactttatt gagaagctcc tgggcctctg
     1141 catccttgcg ttccgctgcg gagtggtttc ggccctcgct gatttcgaca accgccatgg
     1201 gtggcatctt caggccgcgc gcagccgcat tgaaaagatc gaaatagcga ttgaactgcg
     1261 tttgttcggc gccgcttttc aatcggccaa cggccagaat agatatcttc attctgaccg
     1321 tcctatctga tcgggtcgat ctagtctgcg gcttcccgct tcggcctgtc ggagccccac
     1381 atcttttcga ggttgtagaa ctcgcgtgcc tcggggcgga agacgtgaac gatcacatcg
     1441 cccgcatcga taagcaccca atcgcagagg ggcatccctt cgacacgtgt atcgcgaacg
     1501 ccttcctctt tcaacctctc gatgagtcgt tctgcgattg cgcccacatg gcgttgcgag
     1561 cgcccggacg cgacaatcat tgtatcaccg agcgacgtct tgcccgccaa gtcgatgctg
     1621 acaatgttgt cagccttggc gtcctcaagc gcgtcgatga tgaggtgggc gagggaggcg
     1681 gaagccgcgg gggcgcgttt tgcgcggcgc ggtttgagtt ctgcgaactc atgattgaga
     1741 gcttgtggcc tcagcgtaac tacccttctt ctgtattgct ccggctgtgc ttcaggatca
     1801 acacttcgac agagggaatg tgcattacat ttattgaatt ttcaagacag ttcgttagag
     1861 cggcagacct gcaatgaaga aagtactcag gcgatcattg aattagagga ggcagcgtca
     1921 tgctgcctca tccctgcttt ggccgctaac ggcggtgttc gaagccttca tcatcgaagt
     1981 cgccaagccg gtcgaagcag cgctgaagct cgcctgcgcg ctcccgcgct tccatgccga
     2041 gcgattcgcg cctgacgtgc cggcaccagt ggaaatgcgg ctcgacctcg atctcccagc
     2101 gcgggccggt atatccgcac ctgtatttgc ggttcgcttc ggcctgaacc agcgcgacgc
     2161 gtgcgtaggt ctcgcacgcc gcgcgcttgc cttcaacatt gccgcgctcg tcgtcgttga
     2221 agtggaatcc gaagccgcgc gtgcggtgtc cttcggcatc gccgcgctct tcatgccggc
     2281 gctggtcctc gccattgccg cgctcgtctt gcgggggcgt gacttcgatg ctgccgcggt
     2341 cgccgtcgat gcgtaacccg aagccttgcg acagcgccgg gagcgacacc gcgacgcaga
     2401 gtccggcggc tgcgagaact gcgcccgctc ttttgcttac aatcatgacc ataaaaat
//
LOCUS       DATMGX010001099         2343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_123855, whole genome shotgun sequence.
ACCESSION   DATMGX010001099 DATMGX010000000
VERSION     DATMGX010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123855"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VNR65_18030"
     CDS             <1..732
                     /locus_tag="VNR65_18030"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWJ20621.1"
                     /translation="KRRAFDAGEIDETGQPKPERRFYREYADAGAGSRYERAEDLGDL
                     GDFFGDLFGQRARGGEGARAQFRMRGADVNYTLPIDFLEAVNGDKKRVDMPDGKTLNI
                     SIPAGVRDEQVIRLKGQGMPGIGGGPSGDALITVSVKPHPVFRRDGNDIKSALPITLN
                     EALNGGSVQVDTITGPVSLKIPKHSNTGRILRLRGKGVQGHPKGDHHVELQVMMPPHP
                     DAALETFVADWEGEHPYNPRQGGGK"
     gene            729..1073
                     /locus_tag="VNR65_18035"
     CDS             729..1073
                     /locus_tag="VNR65_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20622.1"
                     /translation="MMITETEVIARFTRLNAQVLEHWIAIGWLRPHRGDEGFLFDDAD
                     IARTHLLCDLCYDMELRDEETAMVLSLMDQLHSTRSLLRAMTVAVHSQPNEIREAILT
                     TVRLHFTPRDSN"
     gene            complement(1184..1888)
                     /locus_tag="VNR65_18040"
     CDS             complement(1184..1888)
                     /locus_tag="VNR65_18040"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HWJ20623.1"
                     /translation="MTILAGWGEMTLALTAFFASHVVPARPAVRRRLQAHLGGVAYGA
                     IYTAVSLAVLAWLIAAARNAPHIQLWDFELWQLWVPNLIMPFVCVLIAFGLAAPNPFS
                     IAGRGSAFFDPDRPGIAGITRHPLLWAITLWALAHMVPNGDLAHVLLFGLFAVFGVIG
                     MAALDGRKRKQWGLKLWAERAAKTSTIPFAALFQGRVRLRDLHLDPPRAVAALALYVT
                     LLGAHSHLIGVSPLPS"
BASE COUNT          517 a          706 c          721 g          399 t
ORIGIN      
        1 aagcgccgcg cgttcgatgc cggcgagatc gacgaaacgg gccagcccaa gcccgagcgc
       61 cggttctatc gcgagtacgc cgatgccgga gcgggctccc gctacgagcg cgccgaggat
      121 ctgggcgacc ttggcgactt ctttggagat cttttcgggc agcgagcccg aggcggcgaa
      181 ggcgctcgcg cgcagtttcg catgcgcggc gccgatgtga actacaccct tccgatcgac
      241 tttctcgaag ccgtcaatgg ggacaagaag cgcgtcgaca tgccggacgg caagacgctc
      301 aacatttcga tcccagccgg cgtccgcgac gagcaggtca tccggctcaa agggcaaggc
      361 atgcctggaa tcggcggcgg cccttccggc gatgcgctta tcacggtcag cgtgaagccg
      421 catccggtct tccgccgcga tggcaacgac atcaaatcgg cccttcccat cactttgaac
      481 gaggctctga acggaggctc cgttcaggtc gacaccatca ccggccctgt gagcctgaaa
      541 atcccgaaac attcaaacac cgggcgcatt ctccggctgc gcggcaaagg cgtgcaaggc
      601 cacccgaagg gcgaccacca cgtggaattg caagtgatga tgccgccgca tcccgatgcc
      661 gcgcttgaaa cgttcgtcgc cgattgggag ggcgaacatc cttataatcc gaggcaggga
      721 ggcggaaaat gatgataacc gagacggaag tcatcgcgcg tttcacgcgg ctcaacgccc
      781 aggttctcga acattggatt gcgatcggct ggctgaggcc gcatcgcggc gacgaggggt
      841 tcctcttcga cgacgccgat atcgcccgga cgcatctcct ctgcgacctc tgctacgata
      901 tggaactgcg cgacgaggag acggcgatgg tgctgtcgct catggaccag cttcacagca
      961 cgcgcagcct gctccgcgcc atgacggtgg ctgttcatag tcagccgaac gagatcaggg
     1021 aggcgatcct gaccacggtc cggctgcatt ttacgccccg cgattcaaat tgaacgagtt
     1081 gtcatcccgg aagccgcttg cggctgtccg ggatcgcaaa aatctcagcg cagatgtttg
     1141 taacgacccc ggataagcgc gaggcgcttt ccggggtgac gaatcatgaa ggcagcggcg
     1201 acacgccgat gaggtgggaa tgcgccccaa gcagtgtcac gtagagggcg agcgccgcga
     1261 cggctcgcgg cgggtcaaga tgcaggtcgc gcaagcggac acgtccctga aacaaggcag
     1321 cgaacgggat ggtcgacgtt ttcgctgctc gctccgccca tagcttcaat ccccactgct
     1381 ttcgcttccg tccatccagc gcggccatgc ctatgacgcc gaaaacggca aacaggccaa
     1441 acagcagcac atgagcgagg tctccgttcg ggaccatatg cgcgagcgcc cagagcgtga
     1501 tcgcccaaag gagcggatgc cgtgtgatac cggcgatgcc ggggcgatcg gggtcgaaga
     1561 atgcgctccc gcgccccgcg atggaaaacg ggtttggcgc ggcaaggccg aacgcgatca
     1621 ggacgcacac gaagggcatg atgagattgg gaacccagag ctgccagagt tcgaaatccc
     1681 aaagctgaat gtgaggcgcg ttccgggccg ccgcgatcag ccaggccagc acagcgagcg
     1741 aaacggcggt atagatggcc ccgtaagcga cgcccccgag atgcgcctgc aagcgtcgcc
     1801 gcacggcggg ccgagccggc accacatgcg aggcgaagaa cgctgtcagc gcgagcgtca
     1861 tctctcccca tcctgccagg attgtcatgg tttcgcttcc aatggctcgg tcaacccgtc
     1921 tccaatcggc atcgattaaa ggccgaaaag ttgatcgatt ctagagtgct agaacagctt
     1981 atccgcgtcc caacggacgc gggctgctct agtggttcga tttcgacatt cgcatccgtg
     2041 tgccgcacga tcgagagcaa atgtcgaaat caaaaggacc aggcatgtcc tccggtgagg
     2101 gtatacgaga ggttaaaatt ggttttgttt cgtagaacag ctataagtaa caatataggt
     2161 aatttttgca cgagtcggcg atgggtaaag gcgtcatgac gtatggcggc caccacgcgc
     2221 tctaactcgc tccactagac tgcgatcgga taaagtggta tcgcaaatac cacaaactag
     2281 gaaatacgca agggacagac catggcaacc accgccgaga cgcttcgggc ttatcgcggc
     2341 ccg
//
LOCUS       DATMGX010001100         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_134737, whole genome shotgun sequence.
ACCESSION   DATMGX010001100 DATMGX010000000
VERSION     DATMGX010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134737"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2122
                     /locus_tag="VNR65_18045"
     CDS             <1..2122
                     /locus_tag="VNR65_18045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HWJ20624.1"
                     /translation="PTMRQSPELDVFALSLGLRLLGMPVDPRQIYHGLGRPAAIEEVD
                     ILRAAKAMGAKARAQKTTVSRLQETPLPALAFFKDKGWAVIGKVTPVLAIVQGGGDTC
                     PKTLSLAEFTEAWNGRIILLSKRAALSDPARRFDLTWFWGAITKYRGVLYEVLAASFI
                     IQLFGLATPLIFQTVIDKVLVHRGLSTLEILVAGLALIAVFDIILSGLRTYLFTHTSN
                     RIDVELGSRVFRHLMALPLPYFEARRVGDSVARVRELETIRQFLTGSSITLVLDLLFC
                     AVFIGVMFLYSSLLAWIVVATLPLYAILSIAVTPLFRVRLEEKFHHGAVNQAFLVESI
                     TGVETIKAMAVEPLMQRKWEEQLAAYVGASFRAATLGNVATQATNFLNKATTVLTLFV
                     GAGLIIRNQMTVGELVAFNMLASQVSTPVLRLAQVWQDFHQVRISIARLGDILNTPVE
                     YAPTAPPNTQALEGAIEFEHVQFRYGPGERPALREVSFKIEPGQVIGIVGPSGSGKST
                     IAKIIQRLYLPESGRVLVDGADIHALDPSWLRRQIGVVLQENVLFNASVRDNIALADP
                     GLPIDRVIAAARLAGAHEFISKMREGYDTLVGERGVSLSGGQRQRLAIARALVSDPRI
                     LIFDEATSALDYESESIIHRNMRKIVEGRTVFIIAHRLSAVRLADRILTIEDGVVTED
                     GCHDELAAANGRYATLLRLQSGLA"
BASE COUNT          442 a          682 c          628 g          515 t
ORIGIN      
        1 cccaacgatg agacaatccc cagagctaga cgtattcgcg ctttcgctgg gcttgcgtct
       61 tcttgggatg ccggtggacc cgaggcagat atatcatggc cttggcaggc cagccgcgat
      121 cgaggaagtc gacatcctgc gagcggccaa agcgatgggc gcaaaagcga gggcccaaaa
      181 gaccactgtt tcgcgccttc aggaaacgcc gcttccagct ctcgcctttt tcaaggacaa
      241 gggttgggct gtcattggga aagtaacgcc cgtcctcgct atcgttcaag gtggcggcga
      301 cacttgcccc aagaccctct ctcttgccga gttcaccgaa gcctggaatg gccgcatcat
      361 cctactatcg aagcgcgccg cgcttagcga tcctgcgcgc cgcttcgatc tgacctggtt
      421 ctggggcgcg atcacgaaat atcgtggcgt gctctacgag gtgcttgcag cttccttcat
      481 aatacagctt ttcgggctgg cgacgccgct aatttttcag accgtcatcg ataaggtgct
      541 cgtccaccgc ggcctttcca cactggagat cctggtcgca ggcttagcgc tcatcgccgt
      601 cttcgacatc atcttatcgg ggcttcggac ctaccttttc acgcatacga gcaaccgcat
      661 cgatgtggag ttgggttctc gtgtatttcg ccatttgatg gccctgccgc tcccctattt
      721 cgaggcacgc cgggtgggcg attccgttgc ccgcgtcagg gaactggaaa cgatacgtca
      781 gttcttgacc ggctcatcga ttacgctcgt gctggacctc ctcttctgcg cggtcttcat
      841 cggtgtgatg ttcctttata gttctcttct ggcgtggatc gttgtcgcca ccttgccttt
      901 gtatgcgatc ttgtcgatcg cggtgacgcc attgtttcgc gtccgtctgg aggagaagtt
      961 tcatcacggc gctgtcaatc aggccttcct cgttgaaagc atcacgggcg tcgaaaccat
     1021 caaagcgatg gctgttgagc cgctgatgca acggaaatgg gaagagcaac tcgccgccta
     1081 tgttggagcc tcgttccgcg ccgcgacgct aggcaatgtc gcaacccagg caacgaactt
     1141 cctcaacaag gcgaccacgg tgctcacgct ctttgttgga gcagggctca tcatccgaaa
     1201 tcaaatgact gttggtgaac ttgtcgcctt caacatgctt gcctcgcaag taagcacgcc
     1261 ggtcttgaga ctcgctcaag tttggcagga tttccatcag gtgcggatct caattgcgcg
     1321 gctcggagac atcctcaaca caccggtgga atatgcgccg accgctccgc caaacacaca
     1381 agccttggaa ggcgcgatcg agttcgaaca tgttcagttc cgctatgggc ctggggagcg
     1441 cccggccttg cgagaagtca gtttcaagat cgagccaggg caagtaatcg ggatcgtcgg
     1501 cccatccggt tctggaaaaa gcacaattgc gaaaattatt caacggcttt acttgccgga
     1561 aagtggccgc gttcttgttg atggcgcgga cattcatgcg ctcgatccct cctggctgcg
     1621 ccgccagatt ggcgtcgtcc tgcaagaaaa tgttctgttc aatgccagcg ttcgcgacaa
     1681 catcgccctt gccgatcccg ggctaccaat cgatcgggtc atcgcggctg cgcgccttgc
     1741 cggtgcccac gagtttatca gtaagatgcg cgagggttac gataccttgg ttggcgagcg
     1801 aggcgtgtct ctctccggcg gccagcgtca gcgccttgcc atcgcccgcg cgcttgtgag
     1861 cgacccccgc atacttatct tcgacgaagc cacttccgcc ctcgattacg agtccgaaag
     1921 cattatccat cggaacatgc ggaagattgt cgagggccgc acggtcttca taattgcgca
     1981 ccggctttct gccgtacgcc tggccgaccg tatactcacc atcgaggatg gagtagtgac
     2041 cgaagacggc tgtcatgacg aactcgcggc ggcgaatggc cgttacgcga cactgctgcg
     2101 tctgcaatcg ggcctcgctt agagagatac tttccaatga gatccttctc aggcgacacc
     2161 ctagcaagcc cggatcatag gaagctctct cggaacgagc ggcagttcct tgcgccggcg
     2221 ctcgaaatca ctgagacacc gccatcgcct cttggacgca cggtcac
//
LOCUS       DATMGX010001101         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_92722, whole genome shotgun sequence.
ACCESSION   DATMGX010001101 DATMGX010000000
VERSION     DATMGX010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92722"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..867
                     /gene="rimP"
                     /locus_tag="VNR65_18050"
     CDS             205..867
                     /gene="rimP"
                     /locus_tag="VNR65_18050"
                     /inference="COORDINATES: protein motif:HMM:NF000932.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWJ20625.1"
                     /translation="MERNPSSADVESGAPEAPTERFVRETGSAVEIGALVEPVLEDMG
                     FRLVRVVMSRRDGGTIQIMADKAGGTINVDDCASISRRLSPLLDAHDPIEGRYFLEVS
                     SPGIDRILVRPSDFEDWAGFETKVELKELIDGRRRFRGILEGYENGEMLLQVRLDEES
                     EPQTIGLPVDLIQDAKLVLTDELIRASLARAKAAGKWAEGGEIEDQDIKDSETNDGAD
                     RN"
     gene            848..>2122
                     /gene="nusA"
                     /locus_tag="VNR65_18055"
     CDS             848..>2122
                     /gene="nusA"
                     /locus_tag="VNR65_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWJ20626.1"
                     /translation="MAQIGISANRLELLQIAEAVAREKSIDRTLVLSAMEDAIQKAAK
                     SRYGSENEIRAEIDARTGEIKLSRLLEVVEKVTLDATEISLKDAKKRNPEAEVGDFIA
                     EPLPPLDFGRVAAQNAKQVIVQKVREAERERQHEEYKDRVGEIVSGIVKRAEYGNVMI
                     DLGRAEAIIRRDETLPRENHKLGDRVRAYVYDVRRENRGPQIFLSRTRPEFMAKLFAQ
                     EVPEIYDGIVEIKSVARDPGSRAKIAVVSKDTSVDPVGACVGMRGSRVQAVVNELQGE
                     KIDIIQWSGDAATFIVNALAPAEVAKVVLDEESERIEVVVPDEQLSLAIGRKGQNVRL
                     ASQLTGWDIDILTEAEESERRQKEFAERTNTFVAALDVDEMIAQLLVSEGFTTVEELA
                     YVDEQEVASIEGFDENTAQEIQERAREFLERQE"
BASE COUNT          466 a          583 c          691 g          382 t
ORIGIN      
        1 ccgcaaaccg tgtcggccca ggttgggaca gctcggcatt gagcttctta acagcgtgcg
       61 cacctcctca cgcgaagcct cttgcaaccg gccagcattt ggcctacttt tcggtcagct
      121 caagatcatc tctgattaaa gagtgggcca cccggtccgc tctttttctt tatctggcgc
      181 ttgctgcgct gggaaccgga gtgcatggaa aggaacccgt catcggcgga tgttgaaagc
      241 ggagcccctg aggcgccgac cgagcgtttc gttcgggaga cagggtctgc cgttgagatc
      301 ggcgcgcttg ttgagccggt tctggaggac atgggcttcc gtcttgtgcg cgtggtgatg
      361 tccaggcgcg acgggggtac gatccagatc atggcggaca aggccggcgg cacgatcaac
      421 gtcgacgact gcgcaagcat cagccggcgc ctgtcgccgc tgctcgatgc tcatgacccg
      481 atcgaaggcc gctattttct tgaggtttct tcgcccggaa ttgaccgcat tctggtccgg
      541 ccctccgatt tcgaggattg ggcagggttc gagacaaaag tggagctgaa ggagcttatc
      601 gacggccgcc gccgctttag gggcatattg gaaggctacg agaacggcga gatgctgctg
      661 caagtccggc tcgatgaaga gagcgagccg caaacgattg ggttgccggt cgatctcatt
      721 caagatgcga agcttgtgct gacggatgaa ctcatccggg cttcgctcgc aagagctaaa
      781 gccgccggca aatgggccga gggcggcgaa atcgaagacc aagacatcaa ggattcggag
      841 acgaacgatg gcgcagatcg gaattagcgc aaaccgcctg gaattgttgc aaatcgcgga
      901 agcggtcgcg cgcgaaaagt cgatcgaccg cactctcgtg ctttctgcga tggaagacgc
      961 gatccagaaa gcggccaaat cccgctacgg aagcgaaaac gagatccgcg cggagatcga
     1021 tgcgcgcacc ggcgagatta agctgtcgcg gcttctggaa gtcgtggaga aagtaacgct
     1081 cgacgcaacc gaaatcagcc tcaaggacgc aaaaaagcgt aaccccgagg cagaggtggg
     1141 tgatttcatc gcagagccac tgccgcccct cgactttggc cgtgttgcgg cgcaaaacgc
     1201 caagcaggtc atcgtgcaga aggttcgtga ggccgagcgg gagcgccagc acgaagagta
     1261 taaggatcgc gtgggcgaga ttgtgagtgg catcgtgaaa cgcgccgagt atggcaacgt
     1321 gatgatcgac ctgggccggg ccgaggcgat catccggcgc gacgagaccc tgccacgtga
     1381 gaaccacaag cttggcgacc gcgtccgcgc ctatgtctac gacgtgcgcc gcgaaaaccg
     1441 cgggccgcaa atctttctat cgcgaacgcg gcctgaattc atggcgaaat tgttcgcgca
     1501 agaggtgccc gaaatttacg acggaattgt cgagatcaaa tcagtcgcgc gtgatccggg
     1561 cagccgggca aagatcgccg tcgtctcgaa ggatacttcg gtcgatccgg tcggtgcgtg
     1621 cgtcggcatg cgcggcagtc gcgtgcaggc cgttgtgaac gagctgcagg gcgagaagat
     1681 cgacatcatc caatggtcgg gagacgccgc gaccttcatc gtgaacgcgc tcgctcccgc
     1741 ggaggtggcc aaggttgtgc tcgacgaaga gtcagagcgc atcgaggtcg tggtgccgga
     1801 tgagcagctg agcctcgcca tcggccgcaa ggggcagaat gtgcgcctgg catcgcaact
     1861 gacgggttgg gacatcgata tcctgacaga ggccgaggaa tcggaacgcc gccagaagga
     1921 gttcgccgaa cgcacgaaca ccttcgttgc cgcgctcgat gtcgacgaga tgattgcgca
     1981 gctgcttgtt tccgaaggtt tcaccaccgt tgaagaactt gcctatgtgg acgagcagga
     2041 agtcgcgagc atcgaaggct tcgacgagaa cacggcgcaa gaaatccagg aacgcgcccg
     2101 cgaatttctt gagcggcagg aa
//
LOCUS       DATMGX010001102         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_225115, whole genome shotgun sequence.
ACCESSION   DATMGX010001102 DATMGX010000000
VERSION     DATMGX010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225115"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VNR65_18060"
     CDS             complement(<1..771)
                     /locus_tag="VNR65_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20627.1"
                     /translation="MPAKTILLVLLLYSGLAADNLEKTQKKELEAEVKTMTAEAESLQ
                     QAGQLAEARTKYAESQALIEVKDVTDAIKRLDEEIHRRVKEALNESRKLYEARKFKEA
                     AAVLDQGMKLQAFQPVLAYDLALCYYQLGDRNKAFEYLLKAKAGTEEPKQKQQVSQLL
                     TFFTTGENEASVNDSDGDRIIRANHLVDSIGLEASLEDQGGFEETLSEAGTSSSDTPP
                     VPAAAPAPVKTNQPAVTHSDPNASHRSSLCQAMAELKST"
     gene            1404..>2097
                     /locus_tag="VNR65_18065"
     CDS             1404..>2097
                     /locus_tag="VNR65_18065"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWJ20628.1"
                     /translation="MTHLRKMMLEELERRNYAQTTKDCYLQTIEDFARYFKRPPDQLG
                     PEHIREYQAYLFRERKLAARTVTQRLAGLRFFFIQTMKKPWSVADTPYPKKARHLPSI
                     LSPEEVALLIDSAGTRFHRIVLMTLYGTGARRAELARLQIPDVDSRRMVIHIRGGKGC
                     QDRDVMLSPILLEALRDYWRGLKRKPSQWLFPGGCNHRAGHPITPKTVYHACRQAARR
                     AGLQNRVHPHTLR"
BASE COUNT          425 a          613 c          597 g          462 t
ORIGIN      
        1 agtgctcttg agttcggcca ttgcctggca cagactcgat ctgtgactcg cattgggatc
       61 gctatgggtg acggctggct gattcgtctt caccggcgcc ggggctgccg ctggtacggg
      121 cggagtatcg gaggatgaag tgccggcttc ggagagcgtc tcctcaaatc caccttgatc
      181 ctccaacgat gcttccaggc cgatgctatc gacgaggtga ttggctcgga tgattcgatc
      241 cccgtcgcta tcattcacgg aagcctcgtt ctctcctgtg gtaaagaacg tcagaagctg
      301 agagacttgt tgcttttgct tgggctcctc cgtgccggct ttggctttca gcaagtattc
      361 gaaagccttg ttgcgatcac cgagctggta atagcaaagc gcaagatcgt aggcgagcac
      421 cggctggaaa gcctgcagct tcatgccttg gtccaaaacc gcggcggcct ccttgaactt
      481 acgcgcctca taaagcttgc gcgattcgtt caacgcctcc ttcacccgcc gatgaatttc
      541 ttcatctagg cgcttgatgg cgtccgtgac gtctttcact tcgataagcg cctgggactc
      601 ggcgtacttg gtgcgcgcct ctgccagttg tccggcttgc tgaaggcttt cggcttccgc
      661 agtcattgtc ttgacttcgg cttctagttc ctttttctgg gtcttttcta gattgtcggc
      721 tgccagcccg gaatacagta gaaggaccag gagtatcgtc tttgctggca taagctcctt
      781 tcgggaagct tttcgattgc atcttgtctg ttcgcagaag tactcggggc ctcaacaaaa
      841 aaaccggcgg gttggaccgg gtgaatcccg cgcttcccgc atcaaacgtg gctttatcgc
      901 gtggtgggaa ttggggcaat gacgcgttaa aagccagact ggccgatggg attgagggca
      961 atccaagcat ctattgcccg atcaccccac accccggaca cagtatcagg tgacagggat
     1021 tgtccctaat gaaaactccg tgcagtctgc gcgaagaata agaacctcgc gattgcgact
     1081 gtcgaagcag cggtcggtac tcgaagccga acgtaagcat agcggcgccg cggtgagctt
     1141 cccggcgcac gagtggtcgg ccaacctgaa gttggcccca aaccggaatg tgggagatta
     1201 ctcccgacag gtcggccagt gtttacgaat gccttccttt catctggccg taaaacgccc
     1261 taaccgtaga agcgcgaccc ctgactcccc agaagttggc ttgaacgaag ccgcttcgcg
     1321 gcggacgctt acgcgtgcca ggtgtctgca gcagtgctgt aaacgagagg aagagatagg
     1381 gtgggggcat aaggagatga cttatgaccc accttcgcaa aatgatgctc gaggaactcg
     1441 agcgtcgtaa ttacgcccag actaccaaag actgctacct ccagacgatc gaggacttcg
     1501 cgcgttattt taagcgtcct cctgaccagc tcggtcctga gcacatccgc gagtaccagg
     1561 cgtatctgtt ccgcgagcgg aaactcgccg cacgcaccgt cacgcaacgg ctggccgggc
     1621 tgcggttctt cttcatccag acgatgaaga agccatggag cgtggccgat acgccttatc
     1681 ccaagaaggc gcgacatctg cccagcatat tgagcccaga agaagttgcc ctcctcatcg
     1741 actcggcggg gactcggttt catcgcatcg tgttgatgac gctctatggc accggggcac
     1801 ggcgcgccga gttggctcgc ttgcaaatcc ccgatgtcga cagccgacgc atggtcatcc
     1861 acatccgcgg cgggaaaggc tgtcaggacc gagatgtcat gctcagtccc atcctgctgg
     1921 aagctttgcg cgactactgg cgcgggttaa agcgcaaacc ttcgcagtgg ttgttccccg
     1981 ggggctgtaa ccatagggcg ggtcatccga tcacccccaa gaccgtctat cacgcctgcc
     2041 gccaggccgc ccgacgcgct ggactgcaga atcgggtcca tccccacacc ctgagac
//
LOCUS       DATMGX010001103         2677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_347563, whole genome shotgun sequence.
ACCESSION   DATMGX010001103 DATMGX010000000
VERSION     DATMGX010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347563"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..230)
                     /locus_tag="VNR65_18070"
     CDS             complement(3..230)
                     /locus_tag="VNR65_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20629.1"
                     /translation="MKKIGLTLVVFATLGASTMGMSTAQAAPVAVSGQAPGTDARRQE
                     LIFGNLSTLHAKKSLKDGPTGSSEYEEIGRH"
     gene            complement(346..807)
                     /locus_tag="VNR65_18075"
     CDS             complement(346..807)
                     /locus_tag="VNR65_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20630.1"
                     /translation="MLTLMFRPASRSFVPAVALAAIITVSFFAGAALADSVVLGGTHP
                     PQDIKGACGNANGSFTVNDEGYNCVSSKGSINCNWDGKCIGECDSCGPHGTVVKQGKN
                     TIFGILRGTTLKVGTTVRPVHGPGSSHNPIVAKPIHNHPILERNGSGGKLK"
     gene            complement(870..1346)
                     /locus_tag="VNR65_18080"
     CDS             complement(870..1346)
                     /locus_tag="VNR65_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20631.1"
                     /translation="MKKTTGRSIDQQEETKVRKLFIALFVAAIAVGAFAGGAKAGSKA
                     LAGTYSQSQIEKACSAAGGVPTTKDNGGYGCYATGGAVECTSGGKCVGKCATCGGPAV
                     AHGGKGTVFGVLSGGTLKAGNTIATKNKSAPTTVRRPVAESHQGGTSSTQTHQGKK"
     gene            complement(1738..1881)
                     /locus_tag="VNR65_18085"
     CDS             complement(1738..1881)
                     /locus_tag="VNR65_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20632.1"
                     /translation="MPATARLPSATWSSKLDVLEVVCDKCGRESRYALASLVRYRMVA
                     FGQ"
     gene            1926..2189
                     /locus_tag="VNR65_18090"
     CDS             1926..2189
                     /locus_tag="VNR65_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20633.1"
                     /translation="MRGWGSSRTVTATTPTARGRSPPGEAGNRSGVEAEWSGVLRTVR
                     AGMLAVPSRVQQRLPHLTAHDVAQIDAEVRAALVEVGGRNADA"
     gene            2176..>2677
                     /locus_tag="VNR65_18095"
     CDS             2176..>2677
                     /locus_tag="VNR65_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20634.1"
                     /translation="MQTPEELETGTRLLTIFLPNAIRQRDQIVGANGRFVHYTSAENA
                     LSIMKTKRIWMRNTTCMTDFREVNHGLDALNRCFNDPARLQAFKNAIDGCNAGASDQA
                     INLFNQWWQNTQLETYITSISEHYAREDLHGRLSMWRAFGGSAGRVAIVIKLDLEIGK
                     NAALNVN"
BASE COUNT          555 a          763 c          740 g          619 t
ORIGIN      
        1 ttctagtggc ggcctatttc ttcgtactcg gacgaaccgg tgggaccgtc tttcagcgac
       61 ttcttggcat gaagcgtgct aaggtttcca aaaattagtt cttgccgccg ggcgtcagtg
      121 cccggtgcct ggcctgaaac ggcgaccggt gccgcttgtg ccgtgctcat ccccatcgtg
      181 ctcgcgccga gcgtagcaaa cacgaccaac gtcaatccaa ttttcttcat gacgcttact
      241 ccgattacaa gttcaagatt tggcaaagca ggcacgcgtg tcgacatggt agctctcttt
      301 gactgtacgt ttccgggagg cagaggttca taccaatcaa tcgcgctatt taagtttacc
      361 gccggaaccg tttcgctcaa gaatagggtg gttgtgtatt ggtttcgcaa caattgggtt
      421 gtggctcgat cctgggccgt gcactggccg gacagtagtt cctaccttca gcgtagttcc
      481 ccgtaaaatg ccgaagatcg tattttttcc ctgtttgaca accgtaccgt gggggccgca
      541 gctatcgcat tcgccgatgc atttgccgtc ccagttgcaa ttaatgctgc cttttgaaga
      601 tacgcaatta taaccctcgt cgttaacggt gaaactcccg ttggcgttgc cgcaagcgcc
      661 cttgatgtct tgagggggat gggtaccgcc gagaacaacg ctatcggcaa gcgccgcgcc
      721 agcaaaaaag ctcaccgtga taatggcagc caaagcgacc gcgggtacaa agctgcgact
      781 tgccggtcga aacatcaacg tcaacatacc tgtctcccat gttgcgagtt cgttacaaaa
      841 ttctttcaag cgcctctttg cactttttac tacttttttc cttgatgagt ctgagtgctg
      901 ctcgtgccgc cttgatggct ctctgcgacc ggtcgccgga ccgtggtggg cgcactcttg
      961 tttttagtgg cgatagtgtt tccggccttc agcgtgccgc ctgatagaac tccgaataca
     1021 gtgccttttc ctccgtgcgc gaccgctggg ccgccgcatg tcgcgcattt gccgacacat
     1081 ttgccaccac tggtacattc cacggctccc cccgtcgcgt agcagccgta gccgccattg
     1141 tctttggttg tagggacgcc tccagcggct gaacacgctt tctcgatctg agattgggaa
     1201 tacgttccgg cgagggcctt cgaccctgct ttcgcgccgc cagcaaaggc accgaccgcg
     1261 atagccgcta cgaagagcgc aataaatagt tttctcacct tcgtctcctc ctgttgatcg
     1321 atggagcgtc ctgtcgtttt tttcatttga tccccctgtt gcaaaccgcc gggtatcgta
     1381 ccgccatatt gttagtcggg gcatccaagc cggaggttca acgccgatga aaaaatatcc
     1441 gtcgaatgac gcggtgctgt aatacgtcga acctgccttt ctgtatcgcc aatagccccc
     1501 tatggcttag caggggcgtc attctttcag catcatggat gattgagaag cagggaaacc
     1561 tttagccagg cgcatttata cagaatttat gcggcagccc tgacacgaac cgcccggcca
     1621 tcgcttcggc tcttctcgtc acaaattacc tgggccgggg gtgaattgcg ctctcccact
     1681 tccgctttgg ggtcattcgc gaccgagcca tctcgcgata aaaaccagcc atttccgcta
     1741 ttgtccgaaa gcgaccatcc ggtagcggac gagcgaggcc agtgcgtagc ggctttcgcg
     1801 cccgcacttg tcgcagacga cctcaagcac gtcgagctta ctggaccagg tcgccgaagg
     1861 taatcgcgcc gttgcgggca tcgtgcgtcc tgccgggtgg atattaaggg ggcatcggct
     1921 gtgtcttgag gggctggggc tcaagtcgga cagtgaccgc gacaaccccg acagcccgcg
     1981 gccggtctcc acccggcgag gcgggcaacc ggtccggcgt cgaggccgaa tggtcgggcg
     2041 tgctgcggac ggtgcgcgct ggtatgctgg cggtgccgag ccgggtgcag caacggttgc
     2101 cgcatctgac ggcgcatgac gtggcgcaga tcgacgccga ggtgcgcgcg gcgctggtgg
     2161 aagttggagg caggaatgca gacgcctgag gaacttgaaa ccgggactag gttactgacg
     2221 atctttcttc caaacgctat acgccaaagg gatcagatcg tcggggctaa cgggcggttt
     2281 gtgcattaca catcggctga aaatgcgctg agtataatga aaaccaagcg catatggatg
     2341 agaaacacca cctgtatgac ggattttcgc gaagtgaatc acggtcttga tgcgctaaac
     2401 cgctgcttca atgatcctgc gcgcctacag gcattcaaaa atgcgataga cggctgcaac
     2461 gctggtgcct cagaccaagc tatcaacctg tttaaccagt ggtggcaaaa cacccagctg
     2521 gaaacgtaca tcacatccat ttccgaacat tacgcccgcg aagacttgca cggccgactc
     2581 tcaatgtggc gcgctttcgg tggctcggca ggccgcgttg caatcgtcat taaattggac
     2641 ttggaaatcg gaaaaaacgc agcactaaac gtaaacc
//
LOCUS       DATMGX010001104         2875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_60860, whole genome shotgun sequence.
ACCESSION   DATMGX010001104 DATMGX010000000
VERSION     DATMGX010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60860"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VNR65_18100"
     CDS             <1..435
                     /locus_tag="VNR65_18100"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HWJ20635.1"
                     /translation="RVHRLKAAFPELTIVINGGIATLGEAAAHLDAIDGVMLGRVAYK
                     SPAILGDVDRDLFGEDCLPPVTHAVLDGMLDYVAGELARGTRLSSMTRHMTGLMNGRS
                     GARLFRRLLTEESVKEGAGLDVLRAAIDAARHVEERAARLAA"
     gene            1308..2144
                     /locus_tag="VNR65_18105"
     CDS             1308..2144
                     /locus_tag="VNR65_18105"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWJ20636.1"
                     /translation="MIASLPWSEIAELALALLAGGLVTGFFAGLLGIGGGAIIVPVLY
                     ELFRITGVDDSVRMKICVATSLAIIIPTAIRSVRSHWQRGAVEVSVIKTLGPWVVGGA
                     IVGVFVASHASASFLKGVFVASTLFIAGRLAFGNGSTSAGAKLPGMPWDGLAGAGIGL
                     ISTLIGIGGGAYITAYMKFLGWPIHKAVGTAAGFGPLIAIPAAIGFMAEGWGLPLLPP
                     LSLGYVSLLGLIVVAPVSVLAAPLGVRVAHRLSRRTLEYVFMTFLLVVAARFAASLAF
                     GG"
     gene            complement(2156..2602)
                     /locus_tag="VNR65_18110"
     CDS             complement(2156..2602)
                     /locus_tag="VNR65_18110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HWJ20637.1"
                     /translation="MNNSTEGQETHSQCSPSIVDEHRLLTEKIDAASHEIESFFAASS
                     SQTRLDVKRIIILLEDIRDIAQTHFQHEETFMIKNDFPGAIYHKRDHDYLLKSLIHFT
                     SSLNHGTVPFSPDIGVNLRSWLTYHIKKYDDAYVAFIEASDLKGHD"
BASE COUNT          554 a          799 c          843 g          679 t
ORIGIN      
        1 cgcgtccacc gcctcaaggc ggcattcccg gagctgacaa tcgtcatcaa tggcggcatc
       61 gcgacactcg gcgaagctgc ggcgcacctc gatgccattg atggcgtcat gctcggccgg
      121 gtggcctata agtcgcccgc aatcctggga gacgtcgacc gcgatctgtt cggggaggat
      181 tgtcttcctc cggtcacgca tgcagtcttg gacgggatgc tggattatgt ggcgggagaa
      241 ctcgcgcggg ggacgcggct ctcgtccatg acgcgccata tgaccggcct catgaatggg
      301 cggtcgggcg cgaggctttt ccggcggctt ctcacggagg aaagtgtgaa ggaaggcgcg
      361 gggctcgacg tgctgcgcgc agccatcgac gcggcgcggc atgtagagga aagagccgca
      421 aggctggccg cctgagcaaa ggatcgggct ttgaggccag ccggattgta tgcatccagc
      481 aattattggc atccgcttgc acatttcttc ctgtcacttg cgcccgcctt tcttatcctg
      541 aaagatcctc ggcgaattcg cttttccggc aggttaacgc agcttcaaac ccctaataat
      601 tgcgggaaaa tggcgaacgg gacgccatcg ccgccgcctg cgccgcggct gttttggccg
      661 cggccgctgg ttaattcgca aagctgcaac atatttaacg gatttttgct cgtcccagtt
      721 aaggagaaaa ttcgcgcatt aaattctatt taatggggta atttgtttac taaaaaaact
      781 caattatatc aacgagttat gtccctggta tccgtctaca gactcaggca cggcgcttcc
      841 cctctcgcgg tggtatacaa tatttgacca gaaacgtggc gggaaataat tccggcgcgc
      901 tcctgttaac catgcatcgc ttaaaggcgg tggcgcggcc tcttcgccaa cgcttttttt
      961 acagttgtgg tcaggacttg cgctgaatca tcgatcctgg ccgcgctcgc attgcttgcc
     1021 gcaaagccca ggttttgtcc gagtggcttt ccgagcggaa cggccaagcc aagcgaaagc
     1081 tcgccgttta gaccgagacg gggcaaaccg ggtatttccc gagaccgacc ttgacgggcg
     1141 cgcgcttcag acgccacggc atcggaaagt ccggccacct tcccctctca aacaaactcg
     1201 gtatggcttt tcaatctcgc gtttacagac gacgataggt gattggcgga ggggcagggt
     1261 ggcctgtaat gttctctcat gcccgctaac ccatccgtcg aatctgcatg atcgctagtc
     1321 tgccctggag cgaaatcgcc gagcttgccc ttgcgcttct cgcaggcggg cttgtcaccg
     1381 gctttttcgc gggcctgctc ggcataggcg gcggggctat catcgttccc gttctctatg
     1441 agttgttcag gatcacgggc gtggacgaca gcgtccgcat gaagatctgc gtcgcaacct
     1501 cgctcgccat catcatcccg acagccattc gctcggtgcg ctcgcattgg cagcgaggcg
     1561 cggtggaggt gagcgtcatc aaaacgcttg gcccctgggt ggtgggcgga gccattgtgg
     1621 gcgtcttcgt cgcctcgcac gcgtccgcga gctttctcaa gggcgtcttc gtggcgtcca
     1681 cactgttcat cgccggccgc ctcgcctttg gcaacggctc gacgagcgcc ggcgcaaagc
     1741 ttccgggcat gccctgggac ggcctggcgg gcgcaggcat cggcctcatc tcgacgttga
     1801 tcggcatcgg cgggggcgcc tacatcaccg cctatatgaa gtttttgggt tggccgatcc
     1861 ataaggccgt ggggaccgcc gctggcttcg gtccgctgat tgcgataccg gccgcaatcg
     1921 ggttcatggc ggagggatgg ggcttgccgc tgctgccgcc gctatctctg ggctatgtga
     1981 gccttctcgg gcttatcgtt gtggctcctg tcagcgtgct ggccgcgcct ctcggggtca
     2041 gggtcgcgca ccggctgtct cgccggaccc tggaatatgt gtttatgacc tttctcttag
     2101 tggttgcggc ccggtttgcc gccagcctcg ctttcggtgg ctagcgcgcc gcctctcaat
     2161 cgtgaccctt gagatcgctg gcctctatga aggcgacata ggcatcgtca tatttcttga
     2221 tgtgataggt cagccagctc cgcaagttca cgccgatgtc gggcgagaac ggaacagtcc
     2281 cgtgattgag cgatgatgtg aaatggatca ggctcttgag caaatagtca tgatcacgtt
     2341 tgtgatatat cgctcctgga aaatcatttt ttatcataaa tgtttcttca tgctgaaagt
     2401 gtgtctgggc tatatcccga atatcttcaa gaagaataat aatacgtttt acgtctaaac
     2461 gggtttgaga ggacgatgct gcaaagaagc tttcgatttc atgagacgca gcatcgattt
     2521 tttctgtaag gaggcgatgc tcatcaacga ttgaaggtga acattgcgaa tgtgtttctt
     2581 ggccttcggt cgaattattc actaagtggt tcctcaataa atggaatcgc tcgcaacgaa
     2641 cgctgtcaca aaagtgtatt gaaagcttca caaatgaggc tgtcaaagca caaaaacgtc
     2701 agttccacac cattttaatt caccgtctgc agcgacgttc gtaaacgagg agaccggctg
     2761 agcaggctgc gatgtgtcgc gtatccggcc gagggccacg cgaacctctc tctcttgtga
     2821 tgggaggccg ggttccgcag gctggcgatt cggttattgt cggcccagat caaag
//
LOCUS       DATMGX010001105         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_347924, whole genome shotgun sequence.
ACCESSION   DATMGX010001105 DATMGX010000000
VERSION     DATMGX010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347924"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VNR65_18115"
     CDS             complement(<1..276)
                     /locus_tag="VNR65_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20638.1"
                     /translation="MAYLDEEQHRRARLRNIAQSFALVGGIGIITALSAYTLFGQTGV
                     VWALVFIGVFSLIGPRVAPDAIMRMFKARPIDASSGDEIVRLVQTLSQ"
     gene            complement(363..1016)
                     /locus_tag="VNR65_18120"
     CDS             complement(363..1016)
                     /locus_tag="VNR65_18120"
                     /inference="COORDINATES: protein motif:HMM:NF021553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2278 family protein"
                     /protein_id="HWJ20639.1"
                     /translation="MSVRYGVLRGKPGQILHHRPDDPAPHLEVLIDTPAGPWRLAVNV
                     RSDEQTNLCFHTEQNFKHPILSNLPALPQGLTRPAREDRQLRLDYVRGNFFDSSAMRT
                     ADTGSIGDPDALDTLLSNALAGVRDTDGAELFTFGSPWGPEPDKPDKYFGFLPGRGIH
                     NIHMNQGSPPPHDRDDGVWQDGGLILLFPNGDATAFFFAFQSQTWTTDDISGRPLRP"
     gene            complement(1102..1683)
                     /locus_tag="VNR65_18125"
     CDS             complement(1102..1683)
                     /locus_tag="VNR65_18125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWJ20640.1"
                     /translation="MKRLCVYCGSGSGKDPRFKDAASALGRAMAEAQIGLVYGGGGNG
                     MMGTVAQTVRDFGGHVTGIIPASLLEIENALETINERYVTNGFHERKMLMFNLSDGFV
                     ALPGGVGTLEELVEQLTWTQLGHHDKPVFIVNTAGYWDLLLQMFDQMREQLFIRPELE
                     TRYIVVDDVKRVVPEFLKRRPAKRTTAPHLIRA"
     gene            2000..>2231
                     /locus_tag="VNR65_18130"
     CDS             2000..>2231
                     /locus_tag="VNR65_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20641.1"
                     /translation="MRLVLAAVALIAAKVVTIAMPLAYKAAVDALTILPETDASGKAV
                     QIAAVPLALIVAYGVARISMIGFVQLRDVLFTH"
BASE COUNT          449 a          660 c          663 g          459 t
ORIGIN      
        1 ctgggaaagc gtttgcacca gacgcacgat ctcgtcgccg gatgaggcat cgatcggcct
       61 cgctttgaac atccgcatga tggcatccgg cgcaactcgc ggcccgatca gcgaaaacac
      121 gccgataaag acaagcgccc acacgacgcc cgtctgccca aacagcgtgt aggccgaaag
      181 cgcggtgatg atgccaatcc cgccgacaag ggcgaaagac tgcgcaatat ttcggaggcg
      241 agcgcgccgg tgctgctcct cgtccagata cgccatcgga actccattcc cgtaatttca
      301 cgagatatgg ctcgtccgga tgtgttctca agcaagaggc cggtcaattg actttgaagc
      361 cttcaaggcc tcaaaggcct tccgctgatg tcgtcggtcg tccaggtctg gctctggaaa
      421 gcaaagaaaa aggccgtcgc atcgccattc gggaagagca ggatgaggcc gccatcctgc
      481 cacacgccat cgtcgcgatc gtgaggcggg gggctgccct ggttcatgtg gatgttgtga
      541 atgccgcgtc caggcagaaa accgaaatat ttgtccggtt tatcgggctc ggggccccag
      601 ggacttccaa acgtgaaaag ctcggcgccg tcggtgtccc tcacgcccgc caaggcattg
      661 ctcaacaaag tgtcgagcgc gtcgggatcg ccaatggagc cggtgtccgc ggtgcgcatc
      721 gcgctgctgt cgaagaaatt gccgcgcaca tagtcgaggc gcagctggcg atcttcccgc
      781 gcaggccgcg tcagcccctg tggcagcgcc ggaaggttgg aaaggatggg atgtttgaag
      841 ttctgttctg tgtgaaagca cagattcgtc tgctcgtcgg agcggacgtt aacggcgagc
      901 cgccatggcc ccgccggcgt atcgattaag acttccagat gaggggcagg atcgtccggc
      961 ctgtgatgca ggatctgccc cggcttgccc cgtaaaactc cataacgaac agacattatc
     1021 cccccttagc gaacgcccct ctctgcggct tcgtaaaaaa ccttgaggcc aagattgtgg
     1081 cgttcggtga ggcgcgaggt tctaagcgcg gatgaggtgc ggggccgtag ttcgcttcgc
     1141 cggacggcgt ttcaggaact ccggcacgac ccgcttcaca tcgtcgacaa cgatatagcg
     1201 cgtttcaagc tccggacgga tgaacagttg ctcgcgcatc tggtcgaaca tttgcagcag
     1261 caggtcccaa tatccggcgg tattcacgat gaagactggc ttgtcgtgat ggccaagctg
     1321 ggtccaggtc agctgctcga caagctcctc gagcgtgccg acgccgcccg gcaaggccac
     1381 gaagccgtct gacaggttga acatgagcat cttgcgctcg tggaagccgt tggtgacgta
     1441 gcgctcgttg atggtttcaa gggcgttttc gatttcgagc agcgaggccg gtatgatccc
     1501 cgtgacatgg ccgccaaaat cgcggacggt ctgagcgaca gtgcccatca tgccatttcc
     1561 cccgcctccg tagacgaggc caatctgagc ctcggccatc gcccggccaa gagcggacgc
     1621 tgcgtcctta aagcgcggat ccttccctga tccggagccg caatacacac agagacgttt
     1681 cattttcttt tgtttcatag ggcggtcatc acattcaaaa tgagcttggt caagagctta
     1741 accgatttca gcttgcgtcc cgcttaagga agcgaacctc gctaacagcg aaggctcgct
     1801 tatatggaga gggggttttc tcttgccatc catcccctga agcgctttct gggtatttga
     1861 tcaatcaaag aggcattttt tgcgtcatcg cgccgcttcc catcacgcag ccgcccccga
     1921 agcttcggga gagcaacacc tcgccatcct taagtatttg cttccctttg tgtggccgaa
     1981 aggaaggccg gatctgaagg tgcggctggt gctggcggcg gtggcgctga tcgcggcaaa
     2041 ggtggtcacg attgcgatgc cgctggccta taaagcggcc gtggacgcgc tcaccatcct
     2101 tcccgaaacc gatgcgtcgg gcaaggcggt ccaaatcgcg gctgtccccc tcgccttgat
     2161 cgtcgcttac ggggttgcgc gcatttcgat gatcggcttc gtccagctgc gcgacgtgct
     2221 cttcacgcat a
//
LOCUS       DATMGX010001106         4195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_11645, whole genome shotgun sequence.
ACCESSION   DATMGX010001106 DATMGX010000000
VERSION     DATMGX010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11645"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VNR65_18135"
     CDS             complement(<1..901)
                     /locus_tag="VNR65_18135"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWJ20642.1"
                     /translation="MTGTGQQFIIVGERTNVTGSAKFRKLIEADAYDAALAVAREQVE
                     SGANVLDVNMDEGLLDSEKAMTTFLNLIASEPDISRVPVMIDSSKWAVIEAGLKCVQG
                     KAIVNSISMKEGEEPFLKQARKILRYGAAVVVMAFDESGQAETAERKFAICERAYRLL
                     TERVGFAPEDIIFDPNVFAVATGIEEHNNYGVAFIDAARRIRAGLPHVHVSGGVSNLS
                     FAFRGNEPVRQAMHTVFLYHAIQAGMDMGIVNAGQLGVYDDLNPELRELCEDVVLNRR
                     LDATDRLLEAADRFKGGGSKKQAP"
     gene            complement(986..1426)
                     /locus_tag="VNR65_18140"
     CDS             complement(986..1426)
                     /locus_tag="VNR65_18140"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HWJ20643.1"
                     /translation="MLIDTSVLIALFKDRSGVAARALRDVLDGRDYCLTRFTQMELLQ
                     GARDEKEWLKLSDYLADQDYLEASEETWAAAARLYFDLRRKGLTVRSVIDCCIAEIAM
                     AHGATLLHNDRDFATIAKLRSLKHVQFDAMAAPAFHEPELAPLR"
     gene            complement(1416..1628)
                     /locus_tag="VNR65_18145"
     CDS             complement(1416..1628)
                     /locus_tag="VNR65_18145"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWJ20644.1"
                     /translation="MRTNIDIDDVLIDEVMKVANVKTKKEAVHLALQEFLKVKKKKDL
                     FDLAGKIQFEDNFDHKALRHTRSDAD"
     gene            complement(1699..2499)
                     /locus_tag="VNR65_18150"
     CDS             complement(1699..2499)
                     /locus_tag="VNR65_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20645.1"
                     /translation="MVADHFSVIGFKANSTEALSALVTNLLEKGGEKKPCAAGYYHRL
                     RSAVGPELWVHMQKTAEADSDPYMAVGITPFFEGEGRAPIRVRAMRQRPDDNLFEGAF
                     YVELGPTIALIDVVDFARFAGKPVPFMAMAQIVAFPHALRIFESVAAFQAKQASETIK
                     IAPRSFLASGLFAGGNGSGDVVFLDPEAEDFRAPARAFLTGEVQKTERRRNPETGQEF
                     TWALIGTAGGTFDLVADDAVLPCSLRPGMIVEGEFWLCGRVVEAFGAA"
     gene            complement(2531..3646)
                     /gene="leuB"
                     /locus_tag="VNR65_18155"
     CDS             complement(2531..3646)
                     /gene="leuB"
                     /locus_tag="VNR65_18155"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWJ20646.1"
                     /translation="MTTKTLLLLPGDGIGPEVMTEVKRVIAWLGKSGRAHFEVQEDLV
                     GGCAYDAHGEAISDAAMEKAKAADAVLFGAVGGSKWDKVPYAKRPEAGLLRLRKDLQL
                     FANLRPAICYPALTEASSLKREVVEGLDLMIVRELTGGVYFGEPKEIVTMENGEKRAV
                     DTQIYAEHEIERIARVAFDLARARNKRVASAEKRNVMKTGVLWNAVVTRVHAEYPDVE
                     LTHILADNCAMQLVRWPKQFDVIVTDNLFGDILSDQAAMATGSLGMLPSASLGAKDAS
                     GKRPALYEPVHGSAPDIAGRGIANPIATIASFGMALRYSFGMGKEADLLDASIANVLA
                     KGLRTQDIMQDGMTKVSTAEMGEAVVSELDRLARGAT"
     gene            complement(3842..>4195)
                     /locus_tag="VNR65_18160"
     CDS             complement(3842..>4195)
                     /locus_tag="VNR65_18160"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20647.1"
                     /translation="ISNKMRAYRLQDQGFDTYDADEMLGFGQDQRRFDFAAGMLKQLG
                     FKSVRLMTNNPRKIEALREAGLDVVSTHRVLTRPTAHNEKYLSTKRDKGGHLLGDMAL
                     DGVDLGDLLTPAPQQ"
BASE COUNT          798 a         1300 c         1236 g          861 t
ORIGIN      
        1 cgggggcctg ctttttcgag ccgccaccct tgaacctgtc cgctgcctcc agcaaccggt
       61 ccgttgcgtc cagccgccgg ttgaggacaa cgtcctcgca aagttcgcgt agttccgggt
      121 tcaggtcgtc atacacgcca agctggccag cattcacgat gcccatgtcc atgccggcct
      181 ggatcgcgtg gtacaggaag accgtgtgca tcgcctgccg gaccggctcg ttgccgcgga
      241 aggcgaagga aaggttggag acgccgcccg agacgtgcac atgcggcagc ccggcgcgga
      301 tgcggcgtgc cgcatcgatg aaggcgacgc cgtaattgtt atgctcctca atgccggttg
      361 cgaccgcgaa gacgttcgga tcgaagatga tgtcctcggg cgcgaagccg acgcgttctg
      421 tgagaagcct ataggcgcgc tcgcagatcg cgaatttgcg ctcagccgtc tcggcctgcc
      481 cggactcatc gaacgccatc acgacgacgg ccgctccata gcgcaaaatt ttgcgggcct
      541 gcttcagaaa tggctcttcg ccttccttca tgctgatgga attgacgatc gccttgccct
      601 gcacgcattt aaggcccgcc tcgatcaccg cccatttgga ggaatcgatc atcaccggaa
      661 cgcgggaaat atccggctcc gaggcgatga gattgaggaa ggtcgtcatg gccttctcag
      721 agtcgagcag gccctcgtcc atgttcacgt cgagcacatt cgcgccgctc tccacctgct
      781 cgcgcgcaac cgccagcgct gcgtcgtagg cgtccgcctc tataagcttt cggaattttg
      841 ccgagccagt gacgttggtt cgctcgccga cgataatgaa ttgctggcct gtgccggtca
      901 tgatcagagt gttcctttgc ccccaccgcg ccgtggacct tggtgagcaa cgcggaaaga
      961 ttcaagcaaa atcgaggtcg gaaggttagc gcaagggagc cagctccggc tcatggaacg
     1021 cgggcgcggc catggcatca aattgcacgt gcttcagcga gcgcagcttc gcgatggttg
     1081 cgaaatcgcg atcgttgtgg aggagcgtcg cgccatgcgc catggcgatc tccgcgatgc
     1141 agcagtcgat gacgcttcgg acagtcaagc ccttgcgccg cagatcgaaa taaagccgtg
     1201 cggcggcggc ccatgtctct tcgctcgctt cgagataatc ctggtcggcg agataatccg
     1261 aaagcttcag ccactccttt tcgtcgcgtg cgccctgcaa caactccatc tgggtgaagc
     1321 gggtgaggca gtagtcgcgt ccgtcgagga catcgcgaag ggcgcgtgca gcgacgcccg
     1381 atctgtcctt gaagagcgcg atgagcacgc tcgtatcaat cagcatcgga gcgggtgtgt
     1441 ctcagggcct tatgatcgaa gttgtcctca aactggattt tgcctgcgag gtcgaacaga
     1501 tctttcttct tcttgacttt caggaactcc tgcaaagcaa gatgcaccgc ttccttcttc
     1561 gttttcacgt tcgccacctt catgacctcg tcgatgagca cgtcatcgat gtcgatattc
     1621 gtccgcattg aagcccgatc gttcagccgc gcttgtgtat tatacacatt gccatacaca
     1681 agaaaagctc tttttctctt aggcggcgcc gaacgcttcg acgacccggc cacacagcca
     1741 gaactcacct tccacgatca ttccgggccg caagctgcaa ggcagaacgg catcgtccgc
     1801 caccagatcg aaagtgccac cggctgtgcc aatcaaggcc caggtgaact cctgcccggt
     1861 ctccgggttg cgacggcgct cggtcttctg cacctcgccc gtgaggaacg cgcgtgccgg
     1921 ggcgcgaaaa tcctcggctt cgggatcaag aaagaccacg tccccggagc catttccccc
     1981 agcgaagagc ccgctcgcga ggaaggagcg aggcgcaatt tttatggtct cgctggcctg
     2041 cttggcctgg aaggccgcca cactctcaaa tattctgagc gcatggggga atgccacgat
     2101 ttgcgccatt gccatgaagg gcaccggctt gccggcaaag cgggcgaaat ccaccacatc
     2161 gatgagcgca atggtcgggc caagctcgac gtaaaaggca ccctcgaaca ggttgtcatc
     2221 agggcgctgc cgcatggcgc ggacgcggat cggcgcgcgg ccctcgccct cgaagaaggg
     2281 ggtgatgccg acggccatgt aagggtcaga gtcagcctcg gcggttttct gcatgtgcac
     2341 ccataattcg ggacccacgg cagagcgcag cctatgatag tatcctgcgg cacagggctt
     2401 cttttctccg cccttctcaa gaaggttggt taccagcgca gaaagcgcct cagttgagtt
     2461 cgccttgaaa ccgatgacac tgaagtgatc cgccaccacc cggtcttccc ccctttacgc
     2521 ccttgcggct ctaagttgcg cccctcgcca gccggtcgag ttcgctgacg acggcttcgc
     2581 ccatctccgc ggtcgaaacc ttggtcatgc cgtcctgcat gatgtcctga gtcctgaggc
     2641 ccttggcgag cacattcgcg atgctcgcgt cgagaaggtc ggcctctttc cccatgccga
     2701 acgagtagcg aagcgccatg ccgaagctgg cgatggtcgc aatggggttg gcgatgccgc
     2761 gtccggcgat atccggcgcg cttccgtgga cgggttcgta aagcgccggg cgttttccgc
     2821 tcgcatcctt cgcgccgagc gaagccgaag gcagcatgcc aagcgatcct gtcgccatcg
     2881 ccgcctgatc cgagagaatg tcgccgaaaa gattgtcggt gacgatcacg tcgaattgct
     2941 tcggccagcg cacgagctgc atggcgcaat tgtcggcaag gatgtgggtc agttcgacat
     3001 cggggtactc tgcgtggacg cgggtcacaa ccgcgttcca gagcacgccc gttttcatga
     3061 cattgcgctt ttcggcggag gcgacgcgct tgtttcgcgc acgggcaaga tcgaaggcca
     3121 cgcgcgcaat ccgctcgatt tcgtgctcgg cgtaaatctg ggtgtcgacg gcgcgcttct
     3181 cgccgttctc catggtcacg atctctttcg gttcgccgaa atagacgccg cccgtcaatt
     3241 cgcggacgat catgagatcg aggccttcca ccacctcgcg cttgaggctg gatgcttccg
     3301 tcagtgcggg atagcagatg gcgggtcgca ggtttgcgaa aagctgcaag tccttgcgca
     3361 atcgcagaag accagcctcg ggccgcttcg cgtaaggaac cttgtcccat ttcgagccgc
     3421 cgaccgcgcc gaacaggacc gcatccgcag ccttcgcctt ctccatggcg gcatccgaga
     3481 tggcctcgcc atgggcgtcg taggcgcagc cgccgacaag atcctcttgg acttcaaagt
     3541 gagcgcggcc ggactttccg agccatgcaa tcacgcgttt gacttcggtc atgacctcgg
     3601 ggccgatgcc gtcgccggga agaagaagca gtgtttttgt tgtcatgaac caaaaccgca
     3661 ctactgagca ggccgcatcc tagatgccgg cctcgccgct ggctatacac gctgagcgtt
     3721 taatcacaca ctgttgcgca tatcactcgc ttgatggctg ctctgcacag ctttgtggtt
     3781 gccgcgcaaa tcgcaagcaa atcttttgcc tgatgtaaac ctgcgaactg ccagcgattc
     3841 cttattgttg aggcgccggg gtgagcagat cgccgaggtc cacgccatcg agcgccatgt
     3901 cgccaagaag atgaccgccc ttgtcgcgtt tcgttgagag atatttttcg ttgtgcgccg
     3961 tcggccgggt caacacgcgg tgcgtcgaga caacatcgag gcccgcctcg cgcaacgcct
     4021 cgatctttcg cggattgttg gtcatgaggc gcacggactt gaagccaagc tgcttcagca
     4081 tccccgccgc gaagtcgaag cgacgctgat cctggccgaa gccgagcatt tcatcggcat
     4141 cgtaagtgtc gaagccctgg tcctgcagcc ggtaggcgcg catcttgttt gaaat
//
LOCUS       DATMGX010001107         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_460125, whole genome shotgun sequence.
ACCESSION   DATMGX010001107 DATMGX010000000
VERSION     DATMGX010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460125"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1487)
                     /gene="ahcY"
                     /locus_tag="VNR65_18165"
     CDS             complement(60..1487)
                     /gene="ahcY"
                     /locus_tag="VNR65_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWJ20648.1"
                     /translation="MSAVHKSSSGPSDFYVKDISLAEWGRKEMDIAETEMPGLMAVRE
                     QFGKQQPLKGARIAGCLHMTIQTAVLIESLQAVGADVRWASCNIFSTQDHAAAAIAAR
                     GTPVFAFKGENLDEYWEFVDRILDWPGGQPANMILDDGGDATLIVQLGAEAETNPSVL
                     RNPHSEEEESLFKTIKARLERTPGWYSKVKAAIKGVSEETTTGVLRLYQMEKKGVLSF
                     PCINVNDSVTKSKFDNRYGCRESLVDGIRRATDVMMAGKVAVVCGYGDVGKGSAQSLR
                     GAGARVSVTEIDPICALQAAMDGHEVVTLDDAVGSADIFVTATGNRDIITIEHMRKMK
                     DMAIVCNIGHFDNEIQVATLKNLKWTNVKPQVDMIEFPDGHRILLLSEGRLVNLGNAT
                     GHPSFVMSASFTNQVLAQIELWTNTGKGKYENRVYTLPKHLDESVARMHLAKLGVKLT
                     ELSKEQADYIGVPVEGPYKSDLYKY"
     gene            1835..2033
                     /locus_tag="VNR65_18170"
                     /pseudo
     CDS             1835..2033
                     /locus_tag="VNR65_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611877.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          378 a          628 c          597 g          430 t
ORIGIN      
        1 agggtcgggg acggccccgg ataacgccaa tgacgtttcc ggggcgacga tgagcgggat
       61 cagtacttat acaggtccga cttgtacggc ccctcgaccg gcacgccgat gtagtcagcc
      121 tgctccttcg acaactccgt aagcttcacg ccgagcttcg ccagatgcat ccgcgcgacg
      181 ctctcgtcga gatgcttcgg cagcgtgtag acgcggttct catatttgcc cttgccggtg
      241 ttcgtccaaa gctcgatctg cgcgagcacc tggttcgtga acgaggcgct catcacgaag
      301 ctcggatggc cggtggcgtt gccgaggttc acgagccgcc cctcggacag gagcagaatg
      361 cggtgaccat cggggaactc gatcatgtcc acctgcggct tcacattcgt ccatttgagg
      421 ttcttgagcg tggcgacctg gatctcgttg tcgaagtggc caatgttgca gacgatggcc
      481 atgtccttca tcttgcgcat gtgctcgatc gtgatgatgt cgcggttgcc ggtcgcggta
      541 acgaagatgt ccgccgaacc aaccgcatcg tcgagcgtca caacctcgtg accatccatc
      601 gccgcctgca gcgcgcagat cggatcgatt tcggtgacag acacgcgagc gcccgcaccg
      661 cgcagcgact gcgccgagcc cttgcccacg tcgccataac cgcagacgac ggcgaccttg
      721 cccgccatca tcacgtcggt ggcgcggcgg atgccgtcca cgaggctttc gcggcagcca
      781 tagcggttgt cgaacttcga tttcgtcacg ctgtcattga cgttgatgca ggggaaggac
      841 agcacgccct tcttctccat ctggtagagg cggagcacgc ccgtcgtcgt ctcctcggac
      901 acgcccttga tggcggcctt gaccttggaa taccagcccg gcgtgcgctc gaggcgcgcc
      961 ttgatggtct tgaacaggct ctcctcttcc tcgctatgcg ggttcctcag cacggagggg
     1021 ttcgtctcgg cctcagcgcc aagctggacg atcagcgtgg cgtcgccgcc atcgtcgagg
     1081 atcatgttcg cgggctggcc gcccggccaa tccaggatgc ggtccacgaa ctcccaatat
     1141 tcgtcgaggt tctcgccttt gaaggcgaaa accggcgtac cccttgcggc gatggcggcc
     1201 gcggcgtggt cctgcgtcga gaagatgttg cacgaggccc agcgaacgtc cgcgccgacc
     1261 gcctgcaggc tttcgatcag caccgctgtt tggatagtca tgtgcaggca gcccgcgatg
     1321 cgcgcgccct tcagcggctg ttgcttgcca aactgctcgc gcacggccat caggcccggc
     1381 atctcagttt cggcgatatc catctccttg cgtccccatt ccgcaaggct gatgtctttc
     1441 acgtaaaaat cggacgggcc ggatgacgat ttgtggactg cggacacaga tttctcccaa
     1501 agttgtttgc aataggggtg agatttgacc ctttggtagc ctgtgtgaaa gcgaaaagca
     1561 ataaagacat aaacattgct ttatgtcttc cgctgcctgc atcgcttcga gggaaccaaa
     1621 cttccgtcca gagcagcccg cgtccacttg gacgcggata agctgctcga gcaccttaga
     1681 atcgatcaac ttttccgcct ttaatctgga ggtttctgtg agggcgcaag tggtggtcgt
     1741 gcggccatct caaccgtcga cctcatgaaa cctccagatt cccaaatcac tttttctctg
     1801 actcatgaac tgtcggcaat ggggaggccg acagatgcaa cgctacgcct tgcgcgacga
     1861 ccaatggaca aagatcaagg actttctgcc gggtcgggag ggccatgttg gcggcacggc
     1921 ggcggacaac cgcttgtttg tcgatgccat cctctatcga taccgaaccg gcattcccat
     1981 ggcgtgatct gcccgagcgc tatggcgact ggaaagcgac gcacaagcgc ttg
//
LOCUS       DATMGX010001108         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_308144, whole genome shotgun sequence.
ACCESSION   DATMGX010001108 DATMGX010000000
VERSION     DATMGX010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308144"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2172
                     /locus_tag="VNR65_18175"
     CDS             <1..2172
                     /locus_tag="VNR65_18175"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWJ20649.1"
                     /translation="ITFAFLLLILAMRVYLGRFERLFDDHTIFGGVTYTDAHVTLTGM
                     LVVCVALVLGAVITAVNAVRVPRGRWLVAAILPAAVCYVAVQVIGWYVSSFIVKPNEL
                     VREKPYIVYNTDLTRQAYGLNRISQREFPAETTVEAADAANNQATLQNIRLWDWHALQ
                     DTLRQIQEIRTYYDFPDIDIDRYEIDGTTREVMLATRELSVDKLPESSRNWINEKLIY
                     THGYGITMNPVNGFTPEGLPTLVLSNMPVQSTVRSLSVTRPEVYFGEMTNTDVYVKTQ
                     QKEFNYPQGQTNNLTSYEGNGGILLGGFLRRMIIALDQNDLAKLPFSDDVNKNSRLLM
                     RRNIRDRVSALAPFLTYDPDAYIVLGEDGRLSWIMDAFTISDSYPYSTHYPLDNNSIN
                     YMRNSVKVVIDAYDGTTTFYVFDTDDPIIAAYRRIFPTLFKDASTMPAGLRKHVRYPE
                     MLLKLQAEVYGLYHMTDPEAFYNREDLWTVATEVGLGEGGQQTTQAMQPNFVLMKLPG
                     ETGEEFVEILPFTPANRNNLIGWIAGRSDGAQYGTSVVYNFPKTKLVDGPLQIEARID
                     QNAQLSGQLTLWNQQGSHVRRGALLVIPIGRALLYAEPIYLQAERSPMPELRLVVLAL
                     QDRLAYGPTFESALASLFGGAVSSLTAPSTSAAAVPAEPARGATASTALPAADLNALI
                     AEAAKDLADYQRLTAEGKLGEAGQKLEELKRVLDKLNTRPK"
BASE COUNT          533 a          714 c          728 g          523 t
ORIGIN      
        1 attacatttg cgtttctgtt gctgatcctc gcgatgcgtg tctatcttgg gcggttcgag
       61 cggttgttcg acgatcatac gatcttcggg ggtgtgacct acactgacgc gcacgtcact
      121 ctcacaggga tgcttgttgt ctgtgtggcg cttgttctgg gcgctgtgat cactgccgtt
      181 aatgccgtgc gagtgccgcg cggccgctgg ctggtcgcgg caatccttcc ggctgccgtc
      241 tgttatgtcg ccgttcaagt gatcggatgg tatgtaagca gctttatcgt caagccgaac
      301 gaattggttc gggagaagcc ttacattgtc tacaacactg acctaacgcg gcaggcctac
      361 ggactgaatc ggatctcgca gcgcgagttt ccggctgaga cgaccgttga agcggctgac
      421 gcggccaaca atcaggctac gcttcaaaac atccggctgt gggactggca cgctctccag
      481 gacacgctgc gtcaaattca ggaaatccgc acttattatg actttcccga cattgacatc
      541 gatcggtatg agatcgatgg cacgacacgc gaggtgatgc tcgccacgcg cgaactgagc
      601 gtcgacaagc tccccgagag cagccgcaac tggatcaacg agaagctgat ctacactcat
      661 ggctacggca tcacgatgaa cccggttaat ggctttacgc cggaagggct ccccactctg
      721 gttttgagca acatgccggt ccagagcacg gtgcgtagtc tcagcgtgac ccgtccagag
      781 gtttatttcg gcgagatgac caataccgat gtctacgtga agacgcagca aaaggagttt
      841 aattacccgc aaggccagac caacaactta acgtcctacg aaggcaatgg aggtatcctt
      901 ctcggcggct tccttcgccg catgatcatc gcgctcgacc aaaacgacct cgcgaagcta
      961 ccattcagcg atgacgtgaa caagaatagc cgcctcctaa tgcggcgcaa catacgcgac
     1021 cgcgtatcgg cgctggcgcc gttcctcacg tacgatccgg atgcatacat tgtgctcggc
     1081 gaggacggcc gcctgtcgtg gatcatggat gcattcacga tctccgacag ctatccttac
     1141 tcgactcact atcctctcga caacaattcc attaactaca tgcggaacag cgtgaaggtg
     1201 gtgatcgacg cctacgatgg cacgaccacg ttttatgtct ttgatacaga tgacccgatc
     1261 atagctgcct accgccgcat ttttccgacg ctattcaaag atgcgtcgac gatgccggcg
     1321 ggactgcgga aacacgtgcg ttaccccgag atgctgctca aactccaggc ggaagtttac
     1381 ggcttgtatc acatgacgga cccggaggcg ttttacaacc gggaggacct gtggactgtg
     1441 gcgaccgaag tcggcctggg cgagggaggc cagcaaacga cacaggcgat gcagccgaat
     1501 ttcgtgctga tgaaactgcc gggcgaaacc ggcgaagagt tcgtggagat tctgcccttc
     1561 actccggcaa accggaataa cctgatcggc tggatcgccg ggcgcagcga cggcgcacaa
     1621 tacggtacgt cggtcgttta caactttccc aagacgaagc tggtcgacgg gccgctgcag
     1681 atcgaggcga gaatcgatca gaatgcgcag ctttccggac aattgacgtt gtggaatcag
     1741 cagggctcgc atgtgcggcg cggggctctg cttgtgatcc cgatcgggcg ggcgctactg
     1801 tatgcggagc cgatctacct gcaggctgag cgcagtccga tgcctgagtt gcgcctggtt
     1861 gtactcgcac tgcaagatcg gctggcgtac ggaccaacat tcgagtcggc actggcgtcg
     1921 ctttttggcg gagcggtctc gtcactgact gccccatcaa cgagcgccgc ggctgtcccc
     1981 gcggagcctg cgcgaggcgc aacggcatcc accgccctac ctgcagctga tctgaacgcg
     2041 ctcatcgcgg aagccgccaa agaccttgcc gactatcagc gcctgacggc ggagggcaag
     2101 ctcggcgagg ccgggcaaaa gcttgaagag ttgaagcggg tactcgacaa actgaacacg
     2161 cgcccaaaat agacttgctg cggactcgct aggacattcc attgtgtaac agggttgcgc
     2221 ccagtggatg tcagcatggc gatcgacaaa gtccacactt gcacatattg gttcgactga
     2281 tcgggatgct gccaaatggg caacccacaa accaatcaac ggagtcgtcg catcacaggc
     2341 tgggtgacca gggccttcgc atgtctcggg ctcgtcgccg caactacctg ggcggccttc
     2401 tcattactgc gtgtcaacag cctgattgcg gggtttgcgt acgtcttagt tgtactcgtg
     2461 gtagctgcga agtggggctt ggcggaatcg gtcttgac
//
LOCUS       DATMGX010001109         3355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_214488, whole genome shotgun sequence.
ACCESSION   DATMGX010001109 DATMGX010000000
VERSION     DATMGX010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214488"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VNR65_18180"
     CDS             complement(<1..445)
                     /locus_tag="VNR65_18180"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HWJ20650.1"
                     /translation="MSPVLKPALDMSVNVAGIELKNPVMAASGSFGYGVEFEDVVTLH
                     RLGGFVVKGLSREPMAGHPPPRLFETAAGMLNAIGLQNVGVRAFLEEKLPLLNNIKNI
                     VFFCNVFGHSQEEYEDVVRILNDGEGIAGYELNVSCPNTKCGGISF"
     gene            complement(459..>3355)
                     /locus_tag="VNR65_18185"
     CDS             complement(459..>3355)
                     /locus_tag="VNR65_18185"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWJ20651.1"
                     /translation="AARQRFRGELMSFLATDQYKGITLDIEAFPESSQNDYHALVQEL
                     YDELHAKGLKLYIAVPVNDRTFDYSAIAKISDGLILMNYDQHYPGGDSGPVAGQDWFL
                     KNLQDALKVIPREKIICAVGNYGYDWAMKKGQKKGTPPANVHTVSAQDAWLEAEDAET
                     DVNFDDDAMNPHFAYLDDSDVRHDVWFLDGVTVLNQMRAAHALGIDTFALWRLGSEDR
                     SLWAVWDKPSEANAPGKLDTVPPGQDVDIEGQGEVLRIASRPASGERTITVDPNTNLV
                     SDEVFKTMPSPYEVDMYGGTSQGKVAITFDDGPDPTWTPKILDVLKAKNVKATFFLIG
                     LEVEKYPSIAKRIYKEGHEIGNHTFTHPDISNISKRYFEVELNLTERLFEGKLGVKPV
                     LFRPPYSIDQEPDTADQVRPLELSQDMGFITVGDKVDPNDWRDNPRRSANEIVSDVLA
                     NLPPCQAANLACGNIILLHDGGGDRRETVKALGTIIDTLKARGYEIVPVSDLLGKTRA
                     EVMPPISTNERWAAWVDGLSFGMFGFVSSFIIFVFFVGDVLMSGRLVLVGTLAIFDRF
                     HRRKTSFDPSYRPAVAVLVPAYNEEKVIERTVRSVLDSDYKDLRVIVIDDGSTDATLE
                     LARSAFQKEIAAGRVTVLTKLNSGKADALNFGLEQVTEELFVGIDADTLVAPNAISKL
                     VPHFSDPRVGAMAGNAKVGNRVNLWTRWQALEYITSQNFERRALNTLNAVSVVPGAIG
                     AWRTAPVRAAGGYHHDTVAEDADLTMALLQAGYWVNYEDRALAYTEAPTKASGLMRQR
                     FRWSFGIMQAVWKHRAAVKQKGALGWVAIPNMVIFQILLPLVSPFIDIMFVVGAATYF
                     VSKYFHPESANPASFHRLVVYFALFMVIDFIASAIAFTLERQQPGRKRDFLLLGHVWL
                     QRFAYRQLFSLVLIKTLKRAMEGGSFAWDKLERTASVRQPIAVGREREAQ"
BASE COUNT          662 a          986 c         1001 g          706 t
ORIGIN      
        1 caaagctgat tccgccgcac ttcgtgttgg gacaggaaac gttcagctcg taaccggcga
       61 taccttcgcc atcgttgaga atgcgcacca catcttcgta ctcctcctgc gaatgcccaa
      121 aaacgttgca gaagaaaacg atgtttttga tgttgttaag gagtggcagt ttctcttcca
      181 gaaaggcccg cactccaacg ttctgcagtc cgatggcgtt gagcatgccg gcggcagttt
      241 caaacagccg cggcggaggg tggcccgcca tcggttcgcg ggaaagtccc ttcactacga
      301 acccgcctag ccggtgaaga gtgaccacgt cctcgaattc cacgccatag ccaaagctcc
      361 cactggcagc catgacgggg ttcttcagtt caatgccggc gacgttgacg ctcatatcca
      421 gagcgggctt cagaacggga ctcaccaatt gccctccatc actgcgcctc tctttcgcgg
      481 cctacggcaa tgggctgcct cacactggca gtgcgctcca gtttgtccca ggcaaagctg
      541 cccccttcca tggcgcgctt gagcgtcttt atcaacacta gggagaacaa ttggcggtag
      601 gcgaagcgtt gcagccagac gtggcccagc aagaggaagt cgcgttttcg gcccggctgt
      661 tgccgctcga gcgtgaaggc aatggccgaa gcgatgaaat cgatcaccat gaacagcgcg
      721 aagtacacta ccaggcgatg aaagctggcc gggttcgccg attcgggatg gaaatatttg
      781 ctgacgaagt acgtcgccgc tcccacgaca aacatgatgt caataaacgg cgagaccaac
      841 ggcagcagga tctggaagat gaccatgttc ggaattgcga cccagcccag cgctcctttt
      901 tgctttaccg cggcccggtg cttccatacc gcttgcatga tgccgaagga ccagcggaag
      961 cgttgccgca tcagcccgct cgccttagtt ggcgcctcgg tataggccag cgcgcggtcc
     1021 tcgtaattca cccaatagcc tgcctggaga agcgccatgg tgagatcggc atcttcggcg
     1081 acggtgtcgt gatgataacc accggcggcg cgaaccggag cggtgcgcca ggcgccaatg
     1141 gcgccgggga ccacgctcac ggcgttgagc gtgttcagcg cgcgccgctc gaagttctgg
     1201 ctggtgatgt actccaaggc ttgccagcgg gtccacagat tgacccgatt gccgactttg
     1261 gcgttgcccg ccattgcccc gacccgcgga tcgctaaagt gcggcaccag tttcgagatc
     1321 gcattgggcg ccaccagggt gtctgcgtcg atacccacaa ataactcttc cgtgacttgc
     1381 tcgagtccga agttgagcgc gtcggccttg ccggagttca gcttggttag caccgtgacc
     1441 cggccggcag cgatttcttt ctggaaagcg gagcgcgcga gttccagggt tgcgtccgtc
     1501 gagccatcgt cgatcacaat gacacggagg tccttgtaat cggaatccag caccgaacgc
     1561 acggtgcgct ctatgacttt ctcctcgttg taggccggaa cgagtacagc gactgccggc
     1621 cggtagctcg ggtcgaagct ggtcttacgg cgatgaaacc ggtcaaagat ggccagtgtt
     1681 ccgaccagca cgaggcgccc gctcatgagc acgtcgccca cgaagaaaac gaagataatg
     1741 aacgacgaga caaagccgaa catgccgaag ctaagaccat ccacccaggc ggcccagcgt
     1801 tcgttggtcg agatgggcgg catgacttcc gcgcgggtct tgcccagcag atcggagact
     1861 ggcacgatct cgtagccgcg cgccttcagg gtgtcgatga tggtgcccag cgccttgacg
     1921 gtttctctgc gatcgcctcc gccatcgtgt agcaggatga tgttcccgca ggcaagatta
     1981 gcggcctggc agggcggcag gttggccaac acgtcactaa cgatttcgtt tgccgagcgc
     2041 cgcggattat cgcgccagtc gttcgggtca accttgtccc ccaccgtaat aaagcccatg
     2101 tcctgagaga gctccagagg gcgcacctgg tcggcggtat cgggctcctg gtcaatcgaa
     2161 taggggggac ggaagagtac cggcttgacg cctagcttgc cctcgaacag ccgctccgtg
     2221 agattcagtt cgacttcgaa gtagcgcttg gagatgttgc tgatatcggg atgagtgaag
     2281 gtgtggttcc cgatttcatg gccttccttg taaatgcgct tggcaatgct gggatacttt
     2341 tcaacctcca gcccgatgag gaagaaggta gctttgacgt tctttgcttt cagcacgtcg
     2401 agaatcttgg gagtccacgt agggtctggg ccgtcatcga aggtgattgc aactttccct
     2461 tgtgaggtcc cgccgtacat gtcgacttca tacggcgacg gcatggtctt aaagacctca
     2521 tcgctcacca ggttggtatt cggatcgacc gtgatcgtgc gttcgccgga ggccggacgc
     2581 gaggcgatgc gcaggacttc gccttgccct tcgatgtcca catcctggcc gggaggaaca
     2641 gtgtccagct tgcccggcgc attggcctcg gacggcttgt cccacacggc ccacagcgag
     2701 cggtcttccg aaccgagtct ccataatgcg aaggtatcga ttcccagcgc gtgggcggcc
     2761 cgcatctggt tgagcactgt taccccgtcg agaaaccaca cgtcatggcg aacatcgctg
     2821 tcgtcgagat aggcaaagtg tggattcatg gcatcgtcat cgaagttcac gtcggtctca
     2881 gcgtcttcgg cctcaagcca tgcgtcctgc gccgagacgg tgtgcacgtt cgcgggtggc
     2941 gtgcctttct tctgtccctt tttcatcgcc cagtcatagc cgtaattgcc gaccgcgcag
     3001 atgatttttt cccggggaat gaccttgagg gcgtcctgca ggttcttcaa gaaccagtcc
     3061 tgcccggcga ctggaccgga gtcgccgccg gggtagtgct ggtcatagtt catcaggatc
     3121 aagccatcgg agatttttgc gatggcggag tagtcaaacg tccggtcgtt gaccggcacg
     3181 gcgatataca gcttcaggcc cttggcgtga agctcatcgt agagctcttg taccagcgcg
     3241 tggtagtcgt tctgtgagct ttcgggaaat gcctcaatgt ccagcgtgat gcccttgtac
     3301 tggtccgtag ccaggaagct catgagctcg ccgcggaagc gctgacgcgc cgcgg
//
LOCUS       DATMGX010001110         2100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_14173, whole genome shotgun sequence.
ACCESSION   DATMGX010001110 DATMGX010000000
VERSION     DATMGX010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14173"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VNR65_18190"
     CDS             <1..534
                     /locus_tag="VNR65_18190"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HWJ20652.1"
                     /translation="PINGRITVSGYEGSELLVARLLIESGANVPYVGSACPRTRWSDA
                     DREWLEAKGVHVQYRASLEQDIQAVDEFKPDLAIGTTPVVQHAKAAAIPGLYFTNLIS
                     ARPLMGVAGAGSLAQVVNTAIANKGRFDEMKEFFEGVGEGHNAGVWESAPKAPPKKQE
                     LQLKKPSIKMPEEAIGC"
     gene            528..1979
                     /gene="bchZ"
                     /locus_tag="VNR65_18195"
     CDS             528..1979
                     /gene="bchZ"
                     /locus_tag="VNR65_18195"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase subunit Z"
                     /protein_id="HWJ20653.1"
                     /translation="MLIADLDRAGGYWGAVYVFAAIKGLQVIIDGPVGCENLPVTAVL
                     HYTDALPPHELPIVVTGLSEDELGQHGTEGAMKRAHKTLDPKQPSVVVTGSIAEMIGG
                     GVTPEGTNIQRFLPRTIDEDQWQSANRAMMWLWTEFGAKRKAKVKPKAAGAKPRVNII
                     GPCYGVFNMPSDVAEIRRLVEGIGAEVNMIFPLGAHLNEMPRLAEADANICLYREFGR
                     MLCEALDKPYLQAPIGLHSTTKFLRALGELLGLDPEPFIEREKHTTIKPLWDLWRSVT
                     QDFFGTASFAIVANETYARGVRHFLEDEMGLPCTFAISRRAGVKPDNEEARRLVREKA
                     PLLLFGSYNERMYLAECGGRSMYIPASFPGAIIRRHTGTPYMGYAGATFIVQEVCNAL
                     FDALFNILPLGKDLDRIEATPSRLARQLPWDEEAKTLLDEMVEAQPVLVRISAAKRFR
                     DAAEKAASDAGESKVTAKRVGLSIANAVEGRAA"
BASE COUNT          444 a          600 c          649 g          407 t
ORIGIN      
        1 cccatcaacg gccgcatcac ggtgtcgggc tacgaagggt ccgagctact ggtcgcgcgg
       61 cttctcatcg aaagcggcgc caacgttccc tatgtcggca gcgcttgtcc gcggacccgc
      121 tggtccgatg cggaccggga atggcttgag gccaaaggcg tgcatgtgca gtaccgcgca
      181 tcgctggaac aagacattca ggcggtcgat gagttcaagc cggatctcgc catcggtacg
      241 acccctgtcg tgcagcacgc gaaggcggcc gcgatcccgg gcctttactt caccaatctc
      301 atctcggcgc gtccgcttat gggcgtagct ggcgctggct cgctagctca ggttgtcaac
      361 accgcgatcg caaataaggg ccgcttcgat gagatgaagg agttctttga aggcgtcgga
      421 gaaggccata acgccggcgt atgggaaagc gcaccgaaag cgccgcccaa gaagcaggaa
      481 ctgcagctta agaagccgtc cataaaaatg ccggaggagg ccatcggatg ctgatcgctg
      541 acctcgacag agcgggtggc tattggggcg cagtttacgt ctttgctgcg atcaaggggc
      601 tgcaggtcat catcgacggc ccggtgggct gcgaaaacct cccggtcaca gcggtcctcc
      661 actacaccga tgcacttccc ccgcatgagt tgccgatcgt ggtgactggc ctttcggagg
      721 atgaactcgg gcagcatggt accgaagggg ctatgaagcg tgcccacaag accctggatc
      781 cgaagcagcc gagcgtcgtg gtcacggggt ccatcgctga gatgatcggc ggtggggtga
      841 cacccgaagg cacgaatatt cagcgcttcc tgccccggac gatcgatgag gatcagtggc
      901 aaagcgcgaa ccgggccatg atgtggctct ggaccgaatt tggcgccaag cggaaagcaa
      961 aggtaaagcc gaaggcggcc ggcgcgaaac cccgtgtcaa cataatcggc ccatgctacg
     1021 gcgtctttaa tatgccctcg gacgtggcgg aaatacgccg tttggtcgaa ggcatcggcg
     1081 ccgaagttaa catgattttc ccgctcggcg ctcatctcaa tgagatgccg aggctcgcgg
     1141 aagccgatgc gaatatctgc ctttaccgcg agttcgggcg gatgctctgc gaagcgctcg
     1201 acaagcctta cctgcaggcg cctattgggt tgcattcgac gacgaagttc ctgcgtgcgc
     1261 tgggggagct tctcggactc gatcccgagc ccttcattga acgcgaaaag cacaccacga
     1321 tcaagccgtt gtgggatctc tggcgctccg ttacgcagga tttcttcgga acggccagct
     1381 tcgcaattgt tgcgaacgag acttatgctc gcggcgttcg tcacttcctg gaagacgaga
     1441 tggggttgcc ctgcaccttc gccatctcgc gccgagcagg cgttaagccc gataatgagg
     1501 aggcaaggcg tctcgtgcga gagaaggcgc cactcttgct cttcggaagc tacaacgagc
     1561 gcatgtatct cgctgaatgc ggcggccgtt cgatgtacat tcccgcatca tttccgggag
     1621 caataattcg ccggcacacc ggcaccccat acatggggta tgcaggcgca actttcatcg
     1681 ttcaggaggt ctgcaacgcg ctcttcgacg cgcttttcaa tatcttgccc ctaggaaagg
     1741 acctggaccg gatcgaggct acaccctcta gattggctcg gcagctcccc tgggatgagg
     1801 aggcaaaaac ccttctcgac gagatggttg aagcgcagcc ggttcttgtc aggatctccg
     1861 cagcaaagcg gtttcgtgac gctgcagaaa aggcagccag cgacgcaggc gaaagcaagg
     1921 ttacggcgaa acgcgtcggt ttatctatcg ccaatgctgt agaggggagg gccgcatgac
     1981 tttctcaccg cgtatttctg gtcgaggagg gaaggtgagc atgtctgctt atcacccgca
     2041 agcaaacgag ccgcatgcgc gcatcagagc gcgagaggcg aaggaatttc ggcttatgtt
//
LOCUS       DATMGX010001111         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_313428, whole genome shotgun sequence.
ACCESSION   DATMGX010001111 DATMGX010000000
VERSION     DATMGX010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313428"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VNR65_18200"
     CDS             complement(<1..283)
                     /locus_tag="VNR65_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20654.1"
                     /translation="MQLVKTAMTGSTVWLRFADHDDLAKAKEWIDVQVPIAALKIPPK
                     KLGMFAQADVDWGFTKAPSPTPLSPLSGQPVGVVQVAALRHVKTLIDEQI"
     gene            765..1025
                     /locus_tag="VNR65_18205"
     CDS             765..1025
                     /locus_tag="VNR65_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20655.1"
                     /translation="MTNANKVSRRLVLRGAVAAAGAVPVLLLGMGAAYAKVPKQSVSY
                     RETPNGGNACSSCGNFVAPSGCTLVEGPINPDGWCGLFKAKA"
     gene            1375..1641
                     /locus_tag="VNR65_18210"
     CDS             1375..1641
                     /locus_tag="VNR65_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HWJ20656.1"
                     /translation="MDKNKKVSRRVVLRGAVVAAGAVPILLSGITAAHAKVKQTDVHY
                     QQMPKDGQHCSGCANFEAPKSCKLVDGEINPDGWCQLFKAPKKE"
     gene            2220..>2887
                     /locus_tag="VNR65_18215"
     CDS             2220..>2887
                     /locus_tag="VNR65_18215"
                     /inference="COORDINATES: protein motif:HMM:NF020060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20657.1"
                     /translation="MRIERRFTKSGQSPYADIAFRQATSELKNPDGKVVFQLDGFLVP
                     EQWSQVAADILAQKYFRKAGVPARMKRIEENLVPSWLWRSVPDKQALAELPESERYGP
                     ETDARQVFDRMAGAWTYWGWKGGYFTSEEDASAFHDELCYMLAMQMAAPNSPQWFNTG
                     LHWAYGIDGPSQGHHYVDHRTGQLTKSASAYEHPQPHACFIQSVHDDLVNENGIMDLW
                     VREAR"
BASE COUNT          632 a          770 c          795 g          690 t
ORIGIN      
        1 gaatttgttc gtcgatcaat gtcttaacgt gtcgaagggc tgctacctgc acgacgccga
       61 caggttgacc gctcagtgga ctgagggggg taggagaggg ggcttttgta aatccccaat
      121 caacgtcggc ctgggcaaac atcccaagtt tcttaggtgg tatcttcaga gcggcaatcg
      181 gcacctggac atcaatccat tcttttgctt ttgccagatc atcgtggtcg gcaaatcgca
      241 gccatactgt cgagccagtc atcgcggttt ttactaactg cataacgtca atccttacgc
      301 tgaacagtta tttatacttt attagttgca atcacacctt gcaaaaaatc aaaagtcaat
      361 tcgcgaggat ccgcccctcg cccaatcgag agtgggtctc ttccttccga aaccttcgct
      421 cacccaggaa gccgcccaac tttgttacat tgcagaagta gacagaccat ccgaattcaa
      481 gcagcgtcca agacacccca tcgatagtgc gtagatacga gatcatccct gggccgtggt
      541 tttaccgcgg ccctcccttt ccccaactct cgagtcttca acggccacac agctgtagct
      601 gcatcagcgc aaagcttgct ggcttgcgct cgcatgcgat ggccgctttt caatgagcct
      661 gagagagttc catttcgctc ttgaccagcg tcaaccctga gcttgaggtt cgtgatagtg
      721 ttgatcgtct aggtgaggac ttcagtctat agaaagaaaa aacaatgact aacgccaaca
      781 aggtcagccg ccgcctcgtg ctgcgcggag ccgttgccgc ggcaggagcc gtgccggttt
      841 tgcttttggg gatgggagcg gcttacgcaa aagtccccaa gcagtctgtc tcttaccggg
      901 agactcctaa cggtggaaat gcctgctcca gttgcggcaa tttcgttgcg ccctctggat
      961 gcacacttgt cgaagggccg atcaacccgg atggctggtg cggtctcttc aaggcaaaag
     1021 cataggcgcc agaccgctga tgggagcgaa agcgcaactc aggagtgtgt tgcgctttcg
     1081 gctcctactt tagagaacat gattgtataa atttgtatca aatgtgcgtt ttagacttat
     1141 ccacaagacc ttgtagttaa ttataaactt tgaggcgaaa gcttatgctg cagttggaga
     1201 agtccgctca gactcccctt tgatcttctg ctcgtcttct ttcgcatctt gccgatgatg
     1261 caccataaac tgtcattttc ggggggttag aagaaactta attctgtctt aagcccaact
     1321 gttgtgttat gtttcaacgg tctaggtaga cttcactcca aggagatcgt ttggatggat
     1381 aaaaataaaa aagtatcccg ccgcgttgta ttgcgggggg ccgtggttgc agcaggcgcc
     1441 gtaccgattt tgctttcggg gataacagca gcgcacgcta aggttaagca gacggacgtt
     1501 cactatcagc agatgcccaa agacggtcag cattgctctg gttgtgccaa cttcgaggcc
     1561 cccaaatcgt gcaagcttgt cgacggcgaa atcaatccag atggctggtg tcagcttttc
     1621 aaggccccca aaaaagaata ggcccggtaa accctcctaa agcgaagcgc ctcacaacga
     1681 accccttcga cagcggggga aggtcgtttg tggggtgttt ttttgtttgc aagaccggag
     1741 tttgtttgtc cccagattat gcaatccggc cagccacttt ttcacagaat gaccacaaga
     1801 tattgcgttg atccatgctg tcggcacaaa gattggtgct gttgccccat tgtgacagca
     1861 tcgcaaagtc gaatcggcta gtttctggac atgccgccag cgggggcgtc cttcgaggag
     1921 ggcgcggcgg cagctttctc gccagccgtc caggcggctt ctttcacaaa aaaatctagc
     1981 accgtttggc agttctgcac gtcctttctg gatgcgtttt cgcctgcctg tgtgtcggcg
     2041 gttcaccaaa gttgtgcagc gctctgcgcc gtcgtgtctt gttcctttgg ggcttgattt
     2101 tcgtaagcgc tttcgggtga aaccgtaggt gagtttgaga acagtagcgt gagttttgtt
     2161 ctgttgcgtg agtttggggc aactaaggcg gcttgctcgc ctgatacaga gagaactcca
     2221 tgcggatcga acggcgcttc acgaagagcg gtcagtctcc ttatgcggac atcgcgttca
     2281 ggcaggccac gagtgaactg aaaaatcccg acggcaaagt ggttttccag cttgatggct
     2341 ttctggtgcc cgaacagtgg agccaggtag ccgccgatat tctcgcgcaa aaatacttcc
     2401 gcaaggccgg tgtgccggcc cggatgaagc gcatcgaaga gaacctcgtg ccatcatggt
     2461 tgtggcggtc tgtccccgat aagcaggcgc tggccgagtt gcccgagagc gagcgctacg
     2521 gcccggagac cgacgcgcgg caggttttcg accgcatggc gggcgcgtgg acttattggg
     2581 gttggaaggg cggctatttc acgagcgaag aggatgcgag cgccttccac gacgagcttt
     2641 gctacatgct cgcgatgcag atggccgcgc cgaacagccc gcaatggttc aacaccggcc
     2701 tgcattgggc ctatggcatc gacggcccca gccagggaca tcactatgtc gatcaccgca
     2761 ccggccagct gaccaaatcg gccagcgcct atgagcatcc gcagccgcac gcttgtttca
     2821 tccagtcggt gcacgacgat ctcgtgaacg agaacggcat catggatctc tgggtccgcg
     2881 aggcgcg
//
LOCUS       DATMGX010001112         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_188664, whole genome shotgun sequence.
ACCESSION   DATMGX010001112 DATMGX010000000
VERSION     DATMGX010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188664"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..276)
                     /locus_tag="VNR65_18220"
     CDS             complement(100..276)
                     /locus_tag="VNR65_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20658.1"
                     /translation="MCKPIDRRVRVSCCASLTPMTAWRHKRPFPDYPVCELISLGKIG
                     FASSRVAVEITFLA"
     gene            complement(535..1293)
                     /locus_tag="VNR65_18225"
     CDS             complement(535..1293)
                     /locus_tag="VNR65_18225"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWJ20659.1"
                     /translation="MIRPEKLGDAIAGHLEKLILEGVLKPGEKLAPERDLALKLDVSR
                     PSLREALEKLQSKGLIETTKAGTVVAQFLAPLSRPLAELFADKPRVTADYFEYRRIIE
                     GQAAALAATRATDVDRAALKECSERMEKAHEIEDPSQEAAADADLHQLVYESAHNVVL
                     LHVMRVFSELLRNDVLYNRHQLYRRFGVRDQLLKQHLAIAKEILRGDPKAAEKAASDH
                     IHFTFQTIEDIRRDEERVEVSLRRVSRSDLLADS"
     gene            1446..>2359
                     /locus_tag="VNR65_18230"
     CDS             1446..>2359
                     /locus_tag="VNR65_18230"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HWJ20660.1"
                     /translation="MFQQLLTPVGDSLGLSFIAAILPIVAVLVLLGVLRRPAWQAALA
                     GLVAGLIIAVIGWKMPLGLAIDSVLNGAVFALWPVMWIVINALLLYNIAVRSGRFDAF
                     RNWVVGHLPNDRRVILVVVGFCFGALLEGVSGFGTPVAITSSLLILVGYPPLEALVYV
                     LIFNTAPVAFGALGAPVTVLGAVTGLPATALAAMIGRQLPVIAIILPFYVIALYGGWR
                     SIRALWPVLLVAGGSFAISQFAASNLIDYTLTDVLSSLGSLIATLLFLQVWRPAPDPE
                     FAIKNTKAVAGNPATLSIPNWQGWLPWVV"
BASE COUNT          450 a          689 c          667 g          553 t
ORIGIN      
        1 cgcaggcgac gaaacaaccc agacgagctg atcggccgga tggattgctt cgttcgccgc
       61 tgacattacc aatggccttg cgccagcgtg cgcatctgtc tatgccagaa acgtgatctc
      121 cacggcaacg cggctactgg cgaagccgat cttgccgagg cttatcaact cgcagactgg
      181 gtagtcaggg aatggccgct tgtggcgcca agcggtcata ggcgtcagtg aagcgcaaca
      241 agagacgcgc acccggcgat cgatcggctt gcacatgagc caatccgcac ccgagaattt
      301 gcgagaactg cccgcttgag cgaggcggca gaaaaaacga gacccggtgt gcctaggaga
      361 cccgacctcg taaccggaac agtctgtgcg gcaggccgct cggtatcagc gcagtttatg
      421 gaggtttagg gaagccgcgt tgatctgtct caaatggcca actgaggcgc ccttaaccga
      481 ctgttttttt ggcgcgaagc gacaccagta tgtgactgtt ggctcttcac gtgttcacga
      541 atccgccagc agatcggaac gtgaaacgcg acgcagagac acttctacgc gctcctcgtc
      601 gcgacggatg tcttcgatcg tctgaaacgt aaagtggatg tgatcggaag ccgctttctc
      661 tgctgctttg gggtcccctc gaaggatctc cttggcgata gcaaggtgct gtttcaacaa
      721 ctgatcgcga acaccaaatc ggcggtagag ttggtggcgg ttataaagca cgtcgtttct
      781 taacagctcg ctgaacacgc gcatgacgtg cagcagcacg acgttgtgcg ccgactcgta
      841 gactagctgg tgaagatcgg catcggcagc ggcctcctgg ctggggtcct caatctcgtg
      901 ggccttttcc atccgttcgg agcactcttt caatgcagca cgatccacat cggtcgcgcg
      961 cgttgctgcc agcgccgccg cctgcccctc aatgatcctg cggtactcga aatagtcggc
     1021 ggtaacgcgt ggcttgtcgg caaacagttc cgccagcggc cgggacaatg gcgccagaaa
     1081 ttgcgccacg acagttccgg ccttggtcgt ctcgatcagc cccttgctct gaagtttttc
     1141 cagagcttcc cgcaatgagg ggcgcgaaac gtcgagcttg agcgccagat ctcgctcggg
     1201 ggcgagcttt tctcccggtt tcagcacgcc ttcgaggatc agcttctcga ggtgcccggc
     1261 gatcgcgtct ccaagtttct cgggtcgaat cataaggcac tccgcggatt gatagtggtt
     1321 caactttata tccaatccta ggattgatct atatcaaata ccaatagatt aaatggtaat
     1381 aatatcagtc caaatttagt ttggcaaaaa attataacca aatgccctga gcgggaggta
     1441 tcttgatgtt ccagcaactt ttgacacccg taggagacag cctcgggctt tcgttcatcg
     1501 ccgccatcct gccgatcgtc gcagtgctgg tccttctggg tgtattgcgc aggccggcat
     1561 ggcaggcggc tttggcggga ctcgtcgcgg gcctgatcat cgcggtaatc ggttggaaga
     1621 tgccgctcgg tctggccatc gattccgtcc tcaacggtgc ggtcttcgcg ctttggcccg
     1681 taatgtggat cgtgatcaac gcgctcttgc tctacaacat agccgtacgc tccggccggt
     1741 tcgacgcatt ccgaaattgg gtcgtcggcc atctgcccaa cgatcgacga gtcattctgg
     1801 tcgtggtcgg cttctgtttc ggtgcattgc tggaaggcgt ctcagggttt ggaacaccag
     1861 tcgctatcac cagttcgctg ctgatcctcg tgggctatcc accacttgaa gccctcgtat
     1921 atgtcttgat cttcaatacg gctccggttg ccttcggcgc tctcggcgcg ccggtgactg
     1981 tgctcggcgc tgtgaccgga ttgccagcta ctgcgctggc cgcaatgatc ggacgtcaat
     2041 tgcccgttat tgcaatcatt ctgccttttt atgtcatagc cctctacggc ggatggcgct
     2101 ccatccgtgc gctttggccg gttctactgg tcgccggcgg cagtttcgcg atatcgcaat
     2161 tcgcagcctc caatcttatc gactacacgc tgaccgacgt cttgtcctcg ctcggctccc
     2221 tgatcgccac gctgctcttc ctgcaggttt ggcgtcctgc gccggacccg gaattcgcga
     2281 tcaaaaacac gaaggctgtc gccggcaatc ctgccacgtt atctattccc aactggcagg
     2341 gctggttgcc gtgggtcgt
//
LOCUS       DATMGX010001113         2292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_395529, whole genome shotgun sequence.
ACCESSION   DATMGX010001113 DATMGX010000000
VERSION     DATMGX010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395529"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..961)
                     /locus_tag="VNR65_18235"
     CDS             complement(161..961)
                     /locus_tag="VNR65_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20661.1"
                     /translation="MTLRVLLLATAAFNTCVAPVSPAQAAKAASNTSPEIWAVQASAG
                     LEPPFINALQRISSSDRRLLALSAYLRAGGTLAERWSWSQEQLSSYPGTPEGKAATAD
                     IDAVVAAFAVANPGFRLRVNRDLRSLDVQIAHWNVDESVGTAAAALVAALEQRFIGEA
                     SKPNTGQLRSALIEWKPNVAITLAAPGLSPHGQGRAYDFQVERGGQVIAGVDATLASQ
                     QWDAAGWTQKLRAAVSVAGDHFAGPLESPYEPWHYAYAPRADASNRSR"
     gene            complement(1857..>2292)
                     /locus_tag="VNR65_18240"
     CDS             complement(1857..>2292)
                     /locus_tag="VNR65_18240"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWJ20662.1"
                     /translation="LVLSERTEHLDAIKASLGSLVPAPLVLHGRMSKKQRATVITELD
                     SLPADAPRILLASGKLVGEGFDHPPLDTLVLAMPVSWKGTLQQYAGRLHREHAGKADV
                     RIIDFVDTGHPALLRMWVKRQRGYRAMGYRLATEHPTVGLIA"
BASE COUNT          460 a          697 c          669 g          466 t
ORIGIN      
        1 gagaggactg cggaaccagt tttgcgcagc gtattcggta ggcattggcg gtattgcgcc
       61 ggttgagcca tcatcgtgtg gcgcgccgaa gaaataccgt gaatgagcag tgtgtccaga
      121 atcagaacta gcgtgtgcgt agcgctcttc gttcgaggcg tcagcgagat ctattcgacg
      181 catcagccct gggcgcgtac gcataatgcc acggctcata cggtgattcg agcggccccg
      241 cgaagtggtc gccggctacg ctcacggcag cgcgcagctt ctgtgtccag cccgctgcat
      301 cccactgctg gctcgctaag gtcgcgtcaa cgcccgcgat gacttgtcca ccacgttcga
      361 cttgaaaatc atatgctcgc ccctggccgt gcggggagag tccgggcgcg gccaacgtga
      421 tggcgacgtt gggcttccat tctatgagtg cgcttcgcag ttgacccgtg ttgggctttg
      481 aagcctcacc aataaagcgc tgctccagcg cggcgacgag cgcggctgcg gccgttccga
      541 ctgactcgtc cacattccag tgcgcaatct gtacatccag gcttcgtagg tcccgattga
      601 cccgcaacct gaagccggga ttggcgaccg caaatgccgc gactactgca tcaatgtcag
      661 ccgtagcggc tttgccttcg ggagtcccgg gataactgga tagctgttcc tgcgaccagc
      721 tccatcgctc ggccaacgta cctccagcgc gaaggtacgc actcagggca agaagtcggc
      781 ggtcagaact cgatattcgc tgaagagcat tgatgaaggg cggttcaagg ccggcgctcg
      841 cttgaacagc ccatatctct ggagatgtat tgcttgctgc cttggccgct tgcgccggcg
      901 ataccggcgc gacacaggta ttgaatgccg ccgtcgcaag caggaggact cgtagagtca
      961 ccaacctacg gtttgcgctt cccatgttca tgccagcgcc gcgaggtcca aggagaatag
     1021 ccacgcggaa agtactttca cggcgaccta actatcagtt acgcgaccga aacgtcgcgg
     1081 tgaaccgcga cgaatattac caatgacatt cgtgcactcc ttggcctact ggcgcggctc
     1141 gtcaatcatc ggcgttagct tatcgcaggc cgcgaacggc cgcttcgcgg cggtcatttc
     1201 cgtgcggtca gtgccggctc gtggccgaac cgtggatcgt gccaattccg ggtacaacca
     1261 aactccatcg gcttgaggaa aatattggag caatagcgtt cgaactcaat tcggaagacc
     1321 ttaccgagat cggggacgca gtctcgaaga ttccactgaa aggtgatcgg ctgcctgaaa
     1381 gcgccctagc catgactggt cgctgagcgg tcggcgttcc ctctaacgtt gcgaagcgtg
     1441 cggccagaag gccaatgcgt gtcccggtag acttccggta gacctctacg ccggaaaaac
     1501 atcgcgtttt cccgtagaaa aacgcatctt cctttggtaa ccccttggct tcctgatgga
     1561 aaccaaccga acgcagccgt tacgccttct cacccacctg taactccgca gatttgaagg
     1621 gaactccgca cccaccaatc ccgcagtaac catccggatt cttcgccagg tactgctggt
     1681 gatagtcggc gctgaggcga tttatcccac agaaattgcg tggttacgtc gtcgtggtcg
     1741 cttcgtcggt agactttcgg tagacctcaa cgtcgttccg gcggtagacc agcgcaaggc
     1801 tcaaaatagg ccgcagcgtg ccgtcgccac catgtggatg caggcttcgc gagcgatcaa
     1861 gcaatcagac ccacggtcgg gtgttcagtt gccaggcgat accccatcgc ccggtagccg
     1921 cgctgccgct tcacccacat ccgaagcaac gccgggtgac ccgtgtcaac gaagtcgatg
     1981 atccgcacgt cggccttgcc ggcgtgttcc cgatggagac gccccgcgta ctgctgcagc
     2041 gtgcctttcc aggagaccgg catcgccagt accaaggtgt caagcggagg atgatcgaag
     2101 ccttctccga ccagcttgcc gctagccaac agtatgcgtg gggcatctgc cggaagagag
     2161 tccagttccg tgatgacggt tgctcgctgt ttcttcgaca tccggccgtg cagcaccaac
     2221 ggtgcgggaa ccaggctgcc cagggaggcc ttgattgcgt cgagatgctc agttcgctcg
     2281 ctgagcacca gt
//
LOCUS       DATMGX010001114         2194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_145425, whole genome shotgun sequence.
ACCESSION   DATMGX010001114 DATMGX010000000
VERSION     DATMGX010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145425"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VNR65_18245"
     CDS             complement(<1..637)
                     /locus_tag="VNR65_18245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWJ20663.1"
                     /translation="MSAKTNAWAALLLLTALNFLNYIDRNVLFAVQPLIQKEFALSDK
                     QVGFLTSAFFVCYMIAAPLIVPLADRFPRRRIMAAGAFVWSLATLLTAVTNNYDELLL
                     RHVIVGIGEATFVAISPAFLADLFPETVRGRVMGLFYLATPVGSALGYLVGGYLGNRY
                     GWRAPFMVSALPGLLLAFGVLALREPVRGASDHMADSFERGSVRGLLRNRAF"
     gene            962..1588
                     /locus_tag="VNR65_18250"
     CDS             962..1588
                     /locus_tag="VNR65_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20664.1"
                     /translation="MKRLLLFVVCLMLQVTLLAQDSKPATEANAVADSQPTANAKPAA
                     NSDRMFFPKDWYWGWAQFDITPPHNEADPNLCAANAGQFGGVHSQCSAFGRYMISGTV
                     ELRPFGRTVLRRVNFFYDPTFLFGKNIPQTLYTWSWSGIGMEYSWGVGIDLPKRFEFR
                     VTGHPQMQRFGARDQPLGPAWLGPNGPWGRYNAFGIRKYFGTRREGTY"
     gene            complement(1599..1997)
                     /gene="queF"
                     /locus_tag="VNR65_18255"
     CDS             complement(1599..1997)
                     /gene="queF"
                     /locus_tag="VNR65_18255"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWJ20665.1"
                     /translation="MAKSARYTREHAEAGLSAKFREIETWPNQFPGYEIVIDIPEFTS
                     VCPKTGLPDFGTLWIRYMPDKRCLELKSLKEYLIGYRNLGIFQENIVNRVLEDVVRAS
                     KPKWAEVKGEFRPRGGIGTVVIARYPRPRE"
BASE COUNT          471 a          642 c          659 g          422 t
ORIGIN      
        1 aaaaggcccg gttccggagc agtccgcgca cggaaccgcg ctcgaagctg tccgccatgt
       61 gatcgctggc gccgcgcacg ggttcgcgca gtgccagaac accgaacgcc agcagcaaac
      121 cgggtagcgc cgagaccatg aacggggccc gccaaccgta gcggttcccg aggtaaccgc
      181 cgaccagata acccagcgca ctgccgaccg gcgtcgcgag gtaaaaaagg cccattacgc
      241 gtccgcgtac ggtctccgga aaaaggtctg cgaggaacgc cggcgaaatg gccacgaatg
      301 ttgcctctcc aatgccgacg atgacatggc gcagcaagag ttcgtcgtaa ttgttcgtga
      361 ctgcggtcag cagcgtcgcc aggctccaga cgaaggcacc tgccgccatg atccgccggc
      421 gcggaaagcg atcggccagg ggaacgatca gcggcgcggc aatcatgtaa cagacaaaaa
      481 aggccgacgt caaaaagccg acctgcttgt cgctgagggc gaattccttc tggatcagcg
      541 gctgcaccgc aaacagcacg ttgcgatcga tatagttgag aaagttcagc gccgtcagca
      601 gcagtagcgc ggcccaggca tttgtcttgg cagacatggg tgggcggagg ataacacgag
      661 gggcccgcaa ttcgctgttc gctgttcgcc cgcttttcgc tttcacggtt caactccggg
      721 tccgagactg cattcttgac aacgcgaaga gggagacgtg cctgcgcagc cgctgcaaaa
      781 agccttccca tgttgacgat accgagatac tctctcgtgg aggagaatcg gcctgtgaac
      841 gcgcgcggtg gctcctagcc gcgctatctc gaccgtctgc gcccaccggg gcgaagccgg
      901 cgaggcacgg cacttgtcca ctccttctca gtcgcaccca attcatcgtc gtggagagtg
      961 tatgaaacgc ttgctgctgt tcgtggtttg tctgatgttg caggtcaccc tgctggcaca
     1021 agattcgaag ccggcaactg aagcgaatgc cgtggccgat tcgcagccca cggccaatgc
     1081 caaaccagcg gctaattcgg accgcatgtt ctttcccaag gactggtact ggggatgggc
     1141 gcaattcgac ataacgcccc cgcataacga ggccgatccc aacctgtgcg cagccaacgc
     1201 tggacaattc ggtggcgtgc actcacaatg cagcgccttt gggcgctaca tgatctcggg
     1261 gacggtggag ttgcgtccgt tcggtcgcac cgtgctgcgc cgggtcaact ttttttacga
     1321 ccccacgttc ctgttcggca agaatattcc gcagacgctg tacacctggt cgtggagcgg
     1381 gattggtatg gagtactcct ggggcgtcgg aatcgacctg cccaagcgat ttgaatttcg
     1441 cgtaaccggt catccgcaga tgcagcggtt cggagcgcgc gatcaaccct tggggccggc
     1501 atggctgggc ccgaatggtc cctggggccg ctacaacgcg ttcggaatac gtaagtattt
     1561 cgggacccgg cgagagggca cctactaaga cgcaggcact attccctcgg cctcgggtag
     1621 cgcgcgatca cgaccgtgcc gatgcctccg cgcggacgaa actcgccttt gacttcagcc
     1681 catttcggct tgctggcgcg caccacgtcc tcgagaacgc ggttcacgat gttttcctgg
     1741 aagatgccca gattgcggta accgatcagg tattccttca gcgatttcag ttcgaggcag
     1801 cgcttgtcgg gcatgtagcg aatccacagc gtcccgaaat cgggcaggcc agtcttggga
     1861 catacggagg tgaactcggg tatatcaatc acaatctcgt agccgggaaa ctggttcggc
     1921 caggtttcga tttcccgaaa tttagcggag agaccagcct cggcatgttc ccgagtgtaa
     1981 cgtgcgctct ttgccatgga cctattgtag cgatacgcgc tgcgtcttcg gagcacgctc
     2041 gaacatcaga catacagagg cagcaggaaa ctccatagca cgctggactg gaggagacga
     2101 tgctgaacgg gttggcctta gtaactaaaa acccggatac ggccgaagtg gaagttgtgt
     2161 cctacgttat acttataaat tcgagagaaa atgg
//
LOCUS       DATMGX010001115         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_145432, whole genome shotgun sequence.
ACCESSION   DATMGX010001115 DATMGX010000000
VERSION     DATMGX010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145432"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VNR65_18260"
     CDS             complement(<1..914)
                     /locus_tag="VNR65_18260"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWJ20666.1"
                     /translation="MGQTIVEKIAQKHMAEGPARPLRTGDFLSIRPHRCMTHDNTSAV
                     MSKFKGIGAKKVKGSRQLTFALDHDIQNHDEANQKKYRAIEAFAKEQGVDFYPAGSGI
                     GHQIMVERGYVVPGSFVVASDSHSNMYGALGAIGTPIVRTDAAAVWATGEFWWQIPRS
                     IQVVLEGKLLEGVTGKDVIITLCGLYNHDECLNAAIEFTGPGVASLSMDARLSISNMT
                     TEWGPLVGWFPVDDVTIAYMRGVHARLKAIGVERFSEEDIASWEKNPLAPDVDAVYAA
                     RITLDLAQVTPHVSGPDTVQAMQSLAEI"
     gene            complement(932..1399)
                     /locus_tag="VNR65_18265"
     CDS             complement(932..1399)
                     /locus_tag="VNR65_18265"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWJ20667.1"
                     /translation="MDDLKETIASSLTTRYTKLAGVVGELAAPLSDDQFWAKPFAFGN
                     SFGHLVLHLTGNLNYYIGAQIAGTGYVRDRPLEFSDAARPSKVEVLKKFDQAIEMVIG
                     AINSQSAEDWSKEYSAVGADVRDRFNMVLQCATHLHHHIGQMMYLGFELKRKG"
     gene            complement(1410..>2547)
                     /locus_tag="VNR65_18270"
     CDS             complement(1410..>2547)
                     /locus_tag="VNR65_18270"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWJ20668.1"
                     /translation="SDIFPAAMACCERAKSDGKELIVGFVLGHEFEMRLCEAAFPGIR
                     ERGWHHATLTAFVSPIVAGRALRLGWEQIQHAIGISASRHCTMGAVTAGKLTMMKNTV
                     DPMATQSGVLAALLAEKGYSGPEHVIDGKEGLVHCYGPEWKQNLLTDGLGESWRITQC
                     GMKFFPTEALTHAPISAVLDLVKSNDLRPDQVGKIQIRSLARAADILSDPSKYDPHTK
                     ETADHSLPYVIAAALVDRQVTPAQFEMKKIMDPAIRAQLRKVEVVADPEIEKVFPALQ
                     RVIVNITTTDGRKFSKQLDYPKGDPRNPLSDAEIEEKFRALAEGVLSAKAQDKLIDAI
                     WNLEKCASVSKLMALMKADVRKKAAQGKTRAPKPKAKAKSKRRR"
BASE COUNT          498 a          847 c          629 g          573 t
ORIGIN      
        1 tctatttcag ccagcgactg catcgcttgc accgtgtcgg gccctgacac gtggggcgtc
       61 acttgcgcga gatcgagtgt gatccgcgcc gcatagaccg cgtccacatc cggcgctaga
      121 gggttctttt cccagcttgc aatgtcttct tctgaaaacc gctccacccc gatcgccttt
      181 agccgcgcgt gaacgccgcg catatatgcg atcgtaacgt cgtccaccgg aaaccagccg
      241 acgagcggtc cccactccgt ggtcatattc gaaatcgaaa gccgggcatc catcgaaagc
      301 gacgccactc ccggtcccgt gaattcgatc gccgcattca gacattcgtc gtggttatac
      361 agcccacaca acgtgataat aacgtccttg cccgtaacgc cctccagcag tttgccttcg
      421 agcacgacct gaatcgaccg cggaatctgc caccagaact cgcccgtggc ccagaccgcg
      481 gccgcatccg tgcgaactat cggcgttcca atcgcgccta gcgcgccgta catattggaa
      541 tgcgaatccg atgccaccac gaacgaaccg ggaaccacat acccgcgctc gaccatgatc
      601 tggtgtccga tgcctgatcc ggcaggatag aaatccacac cttgctcttt ggcgaatgct
      661 tcaatcgcgc ggtatttctt ctggttcgct tcatcatgat tctggatgtc atgatcgaga
      721 gcgaacgtaa gctgtcgcga acctttcacc ttctttgcac cgatgccctt gaacttgctc
      781 atcaccgccg aggtgttgtc gtgagtcatg cagcggtgcg gacgaatcga caggaaatcc
      841 cccgtccgca gcggccgcgc cgggccttcg gccatgtgct tctgcgcgat cttttcaacg
      901 atggtctgac ccatgatgaa ctcccaatcc ctcagccttt ccgcttcaac tcgaacccca
      961 aatacatcat ctgcccaatg tgatggtgca gatgcgtcgc acactgcagc accatgttga
     1021 acctgtcgcg tacgtccgca cccactgcac tgtattcctt tgaccagtct tccgccgact
     1081 gactgttaat cgcgccgatc accatctcaa tcgcctgatc aaatttcttc aacacttcca
     1141 cctttgatgg ccgcgccgca tcagaaaact ccaacggacg atcgcggaca tagcccgtcc
     1201 ccgcaatctg cgctccgatg tagtagttca agttgcccgt gagatgcagc acaagatggc
     1261 caaagctgtt gccgaatgcg aagggcttgg cccaaaactg atcgtcgctc agcggcgccg
     1321 ccagttcgcc aactacgccc gccaattttg tgtaacgcgt cgtcaggctc gatgcgattg
     1381 tttctttcag atcatccatt cttcgcccct caacgccgcc tcttactctt tgctttcgcc
     1441 ttgggcttcg gcgccctcgt tttgccttgc gccgctttct tcctcacatc ggccttcatt
     1501 aacgccatca gcttcgaaac cgacgcgcat ttttccaagt tccaaatcgc gtcaatcagc
     1561 ttgtcctgtg ccttcgccga aagcacgccc tccgccagcg cacggaactt ttcttcgatc
     1621 tccgcatcgc tcagcggatt tcgcgggtcc cctttcggat agtcgagttg ctttgagaat
     1681 tttcgaccgt cggtggttgt gatgttcaca atcacccgct gcagcgccgg gaaaactttc
     1741 tcgatctccg ggtccgcgac gacttcaact ttccgaagtt gggcgcgaat cgccgggtcc
     1801 atgatcttct tcatctcgaa ttgcgccggc gtcacttgcc ggtctaccag cgccgctgca
     1861 atcacgtagg gcaacgaatg gtccgccgtc tccttcgtat gcggatcgta tttgctcggg
     1921 tcggaaagga tatcggccgc gcgcgccagc gaccggatct gaatcttccc cacttgatcc
     1981 ggacgcaggt cgttgctctt gaccaggtca agcaccgccg aaatcggagc atgggtcagc
     2041 gcctcagtgg ggaaaaattt catcccgcac tgtgtgatgc gccacgattc gccgagcccg
     2101 tccgtcagca ggttctgttt ccactccgga ccgtagcagt gcaccaatcc ttccttaccg
     2161 tcgatcacgt gctcgggacc gctgtagccc ttctcagcca ggagcgccgc cagaacgccg
     2221 ctctgggtcg ccatcggatc gacggtgttc ttcatcatcg tcagcttgcc cgcagtgacc
     2281 gcgcccatcg tgcaatgccg cgaagccgag atcccgatcg catgttgaat ctgttcccaa
     2341 cccaagcgca gagcgcgtcc agccacgatc ggcgagacga acgccgtcag ggtcgcgtga
     2401 tgccaacctc gctcgcggat gccaggaaac gccgcctcgc acaagcgcat ttcgaactcg
     2461 tggcccaaca caaatccgac gatcaattct ttgccatcgc ttttggcgcg ttcgcaacat
     2521 gccatagcgg ccggaaaaat atcggag
//
LOCUS       DATMGX010001116         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_224167, whole genome shotgun sequence.
ACCESSION   DATMGX010001116 DATMGX010000000
VERSION     DATMGX010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224167"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VNR65_18275"
     CDS             complement(<1..720)
                     /locus_tag="VNR65_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWJ20669.1"
                     /translation="MSGNRSISFSLGAALLLCSSSLNLAYAKPFMIVGTDEKVSWDDD
                     GKTVLSAPGKDSVLIVDLANPESPKIVATLPLKNSIVGPPVNLDIDPTGSVALVADSV
                     DVVKDGEALKQVPDNKVYVIDLKASPPKLAATITVGKQPSGLSISPSGKMALVANRGD
                     NSISVLSVNGTDVQVTDTVAMPDSVAHVLFTPDGKRALAVRFPAHKISILDITNNKVA
                     YNKVDLPTGQWPYNVVLTPNGK"
     gene            880..1089
                     /locus_tag="VNR65_18280"
     CDS             880..1089
                     /locus_tag="VNR65_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20670.1"
                     /translation="MEIQAFGYLGLGASNLDDWATFATSTIGMQSVDRAGSMRAFRMD
                     DRSRQAEYDRPTALPLRTRTRAIVR"
     gene            complement(1195..1716)
                     /locus_tag="VNR65_18285"
     CDS             complement(1195..1716)
                     /locus_tag="VNR65_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20671.1"
                     /translation="MEWIAWLKSCVVPALRWFEAHPGTASWLEAVGSIAAIAFVGLFA
                     LLQARRARSHEDLDRIRRAQGLALVLVPVLRGFKPKIEAAIIRESKLEPPDEVLHLLD
                     QLYVLGVAGGFVLQMVAILQAHQRAEAPPNDENGDARSSYNSLAKQRLGNALRYCDDA
                     IDALLKLTRVRTV"
BASE COUNT          449 a          647 c          619 g          457 t
ORIGIN      
        1 cttaccattg ggcgtcagga caacgttata gggccattgg cccgtgggaa gatcaacctt
       61 gttgtaggcg accttgttgt tggtgatatc gaggatggat atcttgtgtg cggggaatcg
      121 caccgccagc gcccgtttgc catccggcgt gaacagcaca tgtgcgacgc tgtcgggcat
      181 cgcaacggta tcggtgacct gaacgtcggt gccattgacc gagagcacgc tgatcgaatt
      241 gtcgccacga ttggcgacca gcgccatctt gccagatggg ctgatgctca gccccgaggg
      301 ctgcttaccc acggtaattg tcgccgccag tttgggcggg ctggccttga gatcgatgac
      361 gtagacttta ttgtccggca cctgtttcag tgcctcgcca tccttaacaa catcgaccga
      421 atccgccact agcgcgaccg aaccggttgg gtcgatgtcg aggttgaccg gaggtccaac
      481 gatcgaattc ttcagcggca gggtggcgac gatcttgggg ctttcgggat tggcgaggtc
      541 gacgatcagg accgagtcct taccgggcgc ggacaggacc gtctttccgt catcatccca
      601 tgagaccttc tcatcggtac cgacgatcat gaacggcttg gcataagcga gattgagact
      661 ggagctgcag agcagtagtg cagctcccaa tgagaagctg attgaacgat tgccagacat
      721 ctggacgatc tccccgtatg accggcctct gatgaaccgg ttttgcggct ttagcctaag
      781 tgtgcaaacg ctatgaggtc aactcagacc ccctatactc tgatggcaat cgcgctcgcc
      841 gccttttacg cttgccggga aaacggggga ggaaggagca tggaaatcca ggcctttggc
      901 tatctcggcc taggagcatc gaacctcgac gactgggcca ccttcgccac atctaccatc
      961 ggcatgcagt ccgtggaccg ggccggctcg atgcgcgcct tccggatgga cgaccgaagc
     1021 cgccaagcgg agtatgatcg cccgacggcc ttgccactgc gaacccggac gagggcaatt
     1081 gtccggtgaa ccccagacta gtgctttcgt ttgtgcccgc aacgaaactc gcccgttaag
     1141 attgataccg gcgccacggg ttaaatccgg aaccggtgta tatcgagcgg gccgtcaaac
     1201 cgttcggacc cgcgtcagct tcaacaatgc gtcgattgca tcgtcgcagt accgaagtgc
     1261 attccccaac cgctgcttcg ccagcgaatt gtaagaggat cgcgcatccc cgttttcgtc
     1321 attcggggga gcttcggccc gctggtgagc ctgaaggatt gcgaccattt gcaagacgaa
     1381 accgccggct acgcctaaaa cataaagctg atccagcaga tgcaacactt cgtccggcgg
     1441 ttcgagtttg ctctctcgga tgatggccgc ttcaattttc ggcttaaacc ctctcagcac
     1501 aggaacgaga accagcgcta acccttgcgc ccttctgatg cgatctagat cctcgtgtga
     1561 tcttgctcga cgtgcctgta agagagcgaa caggccgacg aaggcaatcg cggctatact
     1621 gccgacggct tccagccaac ttgcggtgcc gggatgcgct tcgaaccatc gcagcgcggg
     1681 cacaacgcag ctcttaagcc atgcgatcca ttccatggcc gaaggctaac cccagtgggt
     1741 gccaaatacc atctgcgtaa cgatgcggcc gggatgatca accgacgaat cagagccacc
     1801 gcgctatccc ttccagcatc accagcttct tggcgtgaag gaagtcttga taggacggcc
     1861 ccgccttccg ggccgctttg aattcggcgt tcagttcctt ttcatgccgc catttctttg
     1921 caactcaatg atccgatcgt cggcgacctg aacggccgca aacgcccgcc tgatgaccgt
     1981 ttcgtgcggc gctgcagctt gtccgcccga aaggcaacgt tgagattgac ggcgagcttc
     2041 aacgcctttg ggaatttagg cacaactccg ttgcgatcgc ctatttgtgt ccgcgggcgg
     2101 gatcgtcaaa cccgcgccgc ctgacaatcc gcgaacgggg gcaagatcgc cttctcgctc
     2161 gaatggcaga ac
//
LOCUS       DATMGX010001117         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_348326, whole genome shotgun sequence.
ACCESSION   DATMGX010001117 DATMGX010000000
VERSION     DATMGX010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348326"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..101
                     /locus_tag="VNR65_18290"
     tRNA            26..101
                     /locus_tag="VNR65_18290"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Lys,seq:ctt)
     gene            complement(949..1308)
                     /locus_tag="VNR65_18295"
     CDS             complement(949..1308)
                     /locus_tag="VNR65_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20672.1"
                     /translation="MPAGKGEESRDRNRPLKVFVAERERAEIETRAAATGLSVSAYLR
                     NLGLGFQPHSTLDQKGILALLKVNGDQGRLGGLFKVWLLGQSAPSTEAVEIRTLLTAI
                     EDCQMQLKALIQHLSQF"
     gene            1642..1986
                     /locus_tag="VNR65_18300"
     CDS             1642..1986
                     /locus_tag="VNR65_18300"
                     /inference="COORDINATES: protein motif:HMM:NF036299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HWJ20673.1"
                     /translation="MAEGSSRKRARWGEGRVAYAALAPEIAEALEEGLPMTKVYALFE
                     ARLGMSYRQFIRYASPRAKERDHDPSPRPVQKPTRTEGCPGPSSPRQLPGAGDARGFH
                     FDPSDIDSKKLI"
     gene            1931..>2505
                     /locus_tag="VNR65_18305"
     CDS             1931..>2505
                     /locus_tag="VNR65_18305"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20674.1"
                     /translation="MQEASISIPQTSTARSSSEAETRQGDAAIRKVHFILNSKGGVGK
                     SFVAFLLALYYRHLREPVLCFDADATTATFSRFAALTVTRITLMEGTILNARRFDEML
                     EPILAEDAHCVVATGTSSFVAVSHYLIENDVHEQIRLSGKKVVVHAIIVGGSTLKETL
                     NDLYELARQLPPEVDIVVWLNEHFGLIKQGD"
BASE COUNT          527 a          770 c          724 g          484 t
ORIGIN      
        1 ttatagctag gagacgcggc gaagcgggcg catagctcag ttggtagagc agctgactct
       61 taatcagcgg gtccaaggtt cgaatccttg tgcgcccacc acttaaaagc ccaataaaat
      121 caagggctta agtcgcgaaa agaaagacaa gggaacactc aaggtcaggt tcggggtaac
      181 gggccgggta acaaccgacc caaaaatcca gccccgttaa ccgactcgtt tttccacagc
      241 caaatcgcgc ttgaccgtgg ctgcacatgc gtcaccgtcc cgatcatgaa aatcgtgtgc
      301 gcaattgtgg ctggccatcg gccccgcggt ttacgtgaac atcgaagcca cgccgatgcc
      361 ttggacgccg ttcgccatcg gaagcgtgtt cttggcggcg gccttcgttt atcgcgcttg
      421 ggaaaagcgc tcgttttggt tcgggacgcg cgcttggcaa cgtcgccagc atgaccgccg
      481 aaacggctga cgggtgcagc ccggtcattt cagcggagtg acggcgtcaa cggcaagcgg
      541 aacgcgctta atgaggcccg caaggtgcaa gtccgcttgc cggtgaagtg tccgctgaag
      601 tgctgacgct gctttcctgc ggaagacgca aaaggaagtg gcggcggatt taggcatcgg
      661 tgagcgcact gttaagcaca tcgtggccct tttcaaaaag caggctgcgg aactgaatgc
      721 ggccgcatcc cgacgccgtg aggctgtcac ctaaacctgt tatcgcaggt ccgcggacgc
      781 tcgaaaaacc ccaaatcacg ggaggaggag accctgctcg atcctcctgc cgttcattac
      841 gacatgcctg gaacagcgtc cgcggacatt tcaggccgat tgccaagcgg ccgaatccgg
      901 tcttggcctt atgggacgct aacccgggcg gtcccttttc ttaaaatgtc aaaattgcga
      961 taagtgctgg atgagagcct tgagttgcat ctggcagtcc tcgatggcgg tgagcagcgt
     1021 ccggatctcc acggcctccg ttgacggcgc gctttgcccc aatagccaga ccttgaacaa
     1081 gccgccaagc cggccctgat cgccgttcac cttgagtagg gcgagaatgc ccttctgatc
     1141 caaggtggag tgcggctgga atccaagccc gagatttctg agataggcgg acacagacaa
     1201 gccggtggcg gcggcgcgcg tctcgatctc cgcgcgctcg cgctcggcga cgaaaacctt
     1261 gagcgggcgg ttcctgtcgc ggctttcctc ccccttccct gcgggcacgc tttcgttctc
     1321 ctttccggcg cgcgtcagcg ctctcgtcga gccgaaggcg agtaagaaag gcgaaggccg
     1381 gagggccgaa gccggtgatg tgggcccaca tcacacatct tgccgtcaaa aactttggca
     1441 atctctggcg atcttcgtcc atctttgtcc atcttaggca aactccgtcc atctatggcg
     1501 atctccgtca atcgagcgat ttttcgccat tcctcgccat agatcgccaa agaacgacag
     1561 tgatcgccaa actttgccct tgtacgccac aggccgaaac agataggtca taacctataa
     1621 ctggtgaccg gacaatgggc ggtggcagag ggatcaagca ggaaaagagc acggtggggc
     1681 gagggccgtg tcgcctatgc ggcgctcgcg ccggagatcg ccgaagccct cgaagagggg
     1741 ctgccgatga ccaaggtcta cgcgctcttc gaggcccgcc tcggcatgag ctaccgccaa
     1801 ttcatacgct acgccagtcc tcgagcaaag gagagagacc atgacccatc cccaaggccc
     1861 gttcaaaagc ccactcggac cgaaggctgc ccaggccctt ccagcccccg ccagcttccc
     1921 ggcgccggcg atgcaagagg cttccatttc gatccctcag acatcgacag caagaagctc
     1981 atctgaggcc gagacccgac aaggggatgc ggcgatccgg aaggttcact tcatcctcaa
     2041 cagcaagggc ggggtgggca aatccttcgt cgcctttctc cttgcgctct actaccgcca
     2101 cttgcgcgag ccggttctct gcttcgatgc agatgcaacc acagccacct tctcccgctt
     2161 tgcggcgctg accgtcaccc gcatcacgct catggaaggc acgatcctga atgcgcgccg
     2221 gttcgacgag atgctggagc cgatcctcgc cgaggatgcg cattgcgtcg ttgccaccgg
     2281 cacttcgagt tttgtcgccg tctcccatta tctcatcgaa aacgatgttc acgagcagat
     2341 ccggctgtcc ggcaagaagg tcgtggtgca cgccatcatc gtcggcggct cgaccctgaa
     2401 ggagacctta aacgaccttt acgagctcgc ccgccagctc ccgccggagg tcgacatcgt
     2461 tgtgtggctg aacgagcatt ttgggctcat caagcagggc gacaa
//
LOCUS       DATMGX010001118         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_123594, whole genome shotgun sequence.
ACCESSION   DATMGX010001118 DATMGX010000000
VERSION     DATMGX010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123594"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VNR65_18310"
     CDS             complement(<1..1008)
                     /locus_tag="VNR65_18310"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ20675.1"
                     /translation="MTATILVVDDEPDLEDVVLQHFQKQISDGRIAFVFARDGVDALQ
                     AVAANPQIEVVLSDINMPRMDGLTLLRKLQEEEHKRTTIVVSAYDDMANIRSAMNRGA
                     FDFLTKPIDFNDLEATLERTILHVNMERNSQQRLIKAELAQASLSRYFSPQLASRLAT
                     GADMDGMTAHVRDISVIFTDITDFLSLVEKTSPAILGEMLNEYMFGMTEIVFAHQGTV
                     AKIVGDAIQILFNAPSDQADYGIRAITCANDLDAWAQGFRLRWRSRDVNFGVTRIGVH
                     AGPALVGNFGGDRFFDYTAHGDTMNTVARLEAVNKHLGTRICASAAVVAIAGRFQGRP
                     IG"
     gene            complement(1023..>2558)
                     /locus_tag="VNR65_18315"
     CDS             complement(1023..>2558)
                     /locus_tag="VNR65_18315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWJ20676.1"
                     /translation="GPDLRAEGLGKPGREYWGDVWELIGPDVEKVMAGKGSVWREHRL
                     MPTGQQDSSRHHYWTYSFSPIEDDAGVGGVLVICRDETQEYRTNLALRAREAELARVQ
                     QIGKIGGLEVNLTRGFQNRRSPEYLAIHGLTPESAHETHDDWVRRIHPDDRWATEKRF
                     LDAVAGREDGYSLRYRIIRPSDGEIRWILAKTQIERDENGNAIRLVGAHADVTDQVVA
                     EQALRQSEEKYRTLAEELSLSLSLDKLRAAQDRLVHTEKLASLGQLTAGIAHEIKNPL
                     NFVNNFSRLSGELIDELLELLSDAPLATQLRDEVDGLAHTLQQNLEKISQHGKRADSI
                     VKNMLLHSRSVSGEHRLVDINAVVDETLNLAYHGARAERANFNIALKREYDDRSGKID
                     LYPQEISRVLLNLISNGFYAATNRQASAGIEPTLVVATKDLGDKVEIRVRDNGAGIPA
                     EIRERIYDPFFTTKPTGEGTGLGLSISHDIIVKQHRGTINLETQPNEFTEFIIALPRK
                     LID"
BASE COUNT          541 a          701 c          671 g          645 t
ORIGIN      
        1 gccaataggc cttccttgaa atcttccggc aattgccacc accgctgcgc tcgcgcaaat
       61 gcgtgttcct aagtgtttgt tgacagcctc tagtcgggcg acggtgttca tcgtgtctcc
      121 atgggctgta tagtcgaaaa accggtcgcc accgaaattc ccgacgagcg ccggacccgc
      181 atgcacacct atgcgcgtaa ccccaaaatt gacgtctctc gacctccagc gaagccgaaa
      241 cccttgtgcc catgcgtcaa gatcattggc gcaggtaatg gctcgaattc cataatcagc
      301 ctgatcgctc ggagcgttaa acaagatttg gattgcatct ccgactattt ttgcaacggt
      361 tccttgatgc gcgaacacaa tttcggtcat gccgaacatg tattcgttca gcatctctcc
      421 cagaattgca ggcgatgtct tctctaccag agacagaaag tcggtgatgt cggtaaatat
      481 gaccgaaatg tcacgaacat gggcggtcat cccgtccatg tcagctcccg tcgctagccg
      541 gcttgccaac tgtggagaga aataacgaga aagcgacgcc tgggctagtt cggcctttat
      601 cagtcgttgc tgggagttgc gctccatatt cacatgaaga atagtcctct cgagtgtggc
      661 ctcgaggtcg ttgaaatcga tcggctttgt caggaagtcg aatgcgcctc ggttcatggc
      721 ggagcggatg ttagccatgt cgtcataggc cgaaacaacg atggtcgttc ttttgtgttc
      781 ctcttcctgc agctttcgca atagggttaa tccgtccatc cgtggcatat tgatgtctga
      841 gagaaccacc tcaatctgcg gattggcggc gacggcctga agcgcgtcga ccccgtcgcg
      901 agcgaagacg aatgcaattc ttccatcgct gatttgcttt tgaaaatgct gcagaaccac
      961 atcctcaaga tccggttcgt cgtctacaac aaggatagtc gcagtcatgg tttgccaagg
     1021 cttcaatcga ttaacttacg tggaagcgca atgataaatt cggtgaactc gttcggttgg
     1081 gtttcgaggt tgatggtacc gcgatgctgt ttcacgatga tatcgtgact gatcgacaaa
     1141 cctaaccccg ttccttcgcc agtaggcttc gtggtgaaga agggatcata aatcctctcc
     1201 ctgatctcgg cgggaatacc ggctccatta tcgcggacac gaatctcgac cttgtcgccg
     1261 aggtccttcg tcgccaccac gagcgttggc tctatgcctg ccgaagcctg ccggttagtc
     1321 gctgcataga acccgttgga gatcaagtta agtagaaccc tggaaatttc ctgagggtat
     1381 agatcgatct tgcccgaacg gtcatcatac tcgcgcttta gcgcaatatt gaaattggct
     1441 cgctcagctc gagcgccgtg ataggcgagg ttgagagtct cgtccacgac ggcgttgata
     1501 tcgactagac ggtgttcgcc cgagactgac cgggaatgca ggagcatgtt ctttacgatc
     1561 gaatcggctc gcttaccgtg ctgactgatt ttttcaaggt tctgttgcag tgtatgggct
     1621 aggccgtcga cctcatcacg cagttgagtg gcgaggggag cgtccgacaa gagttcgagc
     1681 aactcgtcga tcaattcccc ggataatctg gaaaaattgt tgacgaaatt aagtggattt
     1741 ttgatttcgt gggcaatacc cgcggtgagc tgacccaacg aggccagttt ttccgtgtga
     1801 accaagcgat cttgcgctgc tcgcagtttg tcaagcgaca gcgacagcga cagctcctcg
     1861 gccaaggtgc gatatttctc ctcactttgc ctgagagctt gttcggctac tacttgatcg
     1921 gtgacgtccg catgagctcc caccaggcga atagcgttgc cattttcatc gcgttcgatt
     1981 tgggtcttcg ccaagatcca gcggatctct ccatcacttg ggcggataat gcggtaccgc
     2041 aaggagtaac cgtcttcacg acctgcaaca gcatcgagga atcgcttttc ggttgcccat
     2101 cgatcatcgg ggtgaattct gcgaacccag tcgtcatgcg tctcgtgcgc ggactcaggt
     2161 gtcagcccgt gaatggctag atattccggc gaccgtcggt tttggaagcc tcttgttaga
     2221 tttacctcaa gtccgccgat tttcccaatc tgctgtacac gggccagctc ggcctcgcgc
     2281 gcgcgtaagg ccagatttgt tcggtattcc tgcgtctcgt cacgacatat aactagaact
     2341 cctccgacgc cggcgtcgtc ctcgatcggg ctgaagctgt aggtccaata atgatgacgt
     2401 gatgaatcct gctgacctgt cggcatcagt cgatgttcgc gccatacgct gcccttgccg
     2461 gccattacct tttctacatc tggtcctatg agttcccaaa cgtcgcccca gtattcgcgg
     2521 cccggtttgc cgagtccttc cgcgcgcaaa tctggccc
//
LOCUS       DATMGX010001119         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_459880, whole genome shotgun sequence.
ACCESSION   DATMGX010001119 DATMGX010000000
VERSION     DATMGX010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459880"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1159)
                     /locus_tag="VNR65_18320"
     CDS             complement(98..1159)
                     /locus_tag="VNR65_18320"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HWJ20677.1"
                     /translation="MLACFDDHVAQRFVNLALACVHREYPNKIAHILSSDADVLPPRK
                     LTPAFYGCFDWHSSVHGHWLLARLVRSFPDAAFVPRARQALRQSLTAENLAQEAAYMR
                     AEGRASFERPYGLAWLLQLAAELREWNDPQAQEMAHNLRPLEAAVQERLRAWLPKLPY
                     PVRSGEHSQTAFALGLMLDYGRVTGDPDCNEIFVAKARHFYLDDKNCPLAYESSGEDF
                     LSPCLAEADVMRRVLAPPDFAGWLRAFLPQIAEDGSGNWLKPMVSPDPSDGKLAHLDG
                     LNLSRAWMLEGIASALPENDARLSAILSAAASHSRAGLAAVTGERYEGGHWLGSFAVY
                     LVSRRGIHEGCTSGSRVIA"
     gene            complement(1168..>2138)
                     /locus_tag="VNR65_18325"
     CDS             complement(1168..>2138)
                     /locus_tag="VNR65_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20678.1"
                     /translation="GRARSRRICGCSHASGHRQNVFTIAQSSHTNFETALELMRKAAI
                     LGFFLLTAASVLAQTAPTPATQTPATPAAWPTAHFASTKDPSVQKALQILNDMIKTLG
                     GDAYLNVRDTKTEGRGYAFYHGQPSGQGLVFWRFWEWPDKERVELTKQRDVIELNVGD
                     KGYEITYKGTATQEPQQLQDYLRRRDHSLEWVIRKWLGASDTMVLYNGTAIVEQNLTD
                     QVTILNSTNDSVTLSVDPRSHLPVRKIYSYRDPLDRQLDDEAEVFSNYRLIQGIQTPF
                     SIVRHHNGEMRSQRFITSVTYNNGFPPTMFETTGLTYNQPKSGEKK"
BASE COUNT          417 a          619 c          672 g          430 t
ORIGIN      
        1 gaccgttttg ggacaggaac gcggcggcga aaggcgggct cacccaaagc ggaagcatcc
       61 gacacagact ccaagcgggg gatggttgta tgcggggcta tgcaatgacc cttgagccgg
      121 atgtgcaacc ttcatgaatc ccccggcggg agacgaggta aaccgcgaag cttcccagcc
      181 agtgaccgcc ttcataacgc tcgccggtga ccgcggcgag tcctgcgcgc gagtgagacg
      241 cagcggcgga caggatcgcg gacagccgtg cgtcattctc cggcagcgcg ctggcaatcc
      301 cttcaagcat ccatgcgcga ctcaggttca agccgtccag gtgcgccagc tttccatcgc
      361 tggggtcggg agagaccatc ggctttagcc agttaccact gccgtcctcg gcgatctgag
      421 gcaggaacgc gcgcagccat ccggcaaagt caggcggcgc aagcacgcgc cgcattacgt
      481 cggcttcggc caagcatggc gagaggaaat cctcgcccga ggactcgtac gccaatgggc
      541 agttcttgtc gtcgagatag aaatggcgag ctttcgcgac gaagatctcg ttgcaatcgg
      601 ggtcgcccgt gacgcgccca tagtccagca tgagtccgag agcaaacgcg gtttgcgaat
      661 gctcacccga gcgcaccgga tagggcagct tcggcagcca cgcccgcagc cgttcctgca
      721 cagccgcttc cagtggacgt aagttgtgcg ccatctcttg cgcttgcgga tcgttccact
      781 cgcgcagctc ggcggccagt tgcagaagcc acgccaaacc ataagggcgc tcgaaactcg
      841 cacggccttc agcgcgcatg tacgccgctt cctgcgcgag gttctccgcg gtcaaacttt
      901 gtcgcaacgc ttggcgtgct cgcggcacaa acgcggcatc gggaaaactt cgcaccaggc
      961 gcgcgagcag ccaatgtccg tgcacggacg aatgccagtc aaaacagccg tagaaagccg
     1021 gggtcagttt gcgcggaggc agaacatcgg catcgctgct taagatgtgg gcgatcttgt
     1081 tgggatattc gcggtggacg caagccaacg ccagattaac gaagcgctgc gcaacgtggt
     1141 cgtcgaagca ggccagcata cgtgtcgcta cttcttctcc ccactcttag gctggttgta
     1201 ggtgaggccc gtggtctcaa acatcgtggg cgggaagccg ttgttgtagg tcaccgacgt
     1261 gatgaagcgt tgactcctca tttcgccgtt atgatgtcgc acgatgctga agggagtttg
     1321 aatgccctga atgaggcgat agttgctgaa aacttccgcc tcgtcatcga gctgccgatc
     1381 gagcgggtcg cggtagctat agattttcct cactggcaag tgggaacgcg gatcgaccga
     1441 caaggtcacg ctatcgttgg tggaattcaa gatggtgacc tggtcggtga ggttctgctc
     1501 cacgattgcg gtcccgttgt acaggaccat ggtgtccgat gcgcccagcc atttgcggat
     1561 tacccattcc aacgagtggt cgcggcggcg cagataatcc tggagctgct gcggttcctg
     1621 ggtcgcggtg cccttgtatg tgatctcgta tcccttatcg ccgacattga gttcgatgac
     1681 gtcacgctgt ttggtcagct cgacgcgctc tttgtcgggc cattcccaga accgccagaa
     1741 tacaagcccc tgcccactcg gttgtccgtg atagaacgca taaccgcggc cttcggtttt
     1801 cgtgtcgcgc acgttgagat acgcatcgcc gcccagggtc ttgatcatgt cgttaaggat
     1861 ctggagagcc ttctgcacgc tggggtcttt ggtcgaagcg aagtgggcgg tgggccatgc
     1921 ggcgggagtg gccggagtct gcgtggctgg cgttggggcg gtctgagcga ggacagacgc
     1981 cgccgtcagg agaaagaagc cgagtatcgc agctttgcgc atgagttcta aagcagtttc
     2041 gaagtttgtg tgactgctct gagcgattgt aaacacattt tggcggtggc cgctcgcgtg
     2101 cgaacaaccg catatccttc gactacgcgc ccgcccga
//
LOCUS       DATMGX010001120         2758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_189305, whole genome shotgun sequence.
ACCESSION   DATMGX010001120 DATMGX010000000
VERSION     DATMGX010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189305"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VNR65_18330"
     CDS             complement(<1..198)
                     /locus_tag="VNR65_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20679.1"
                     /translation="MVKEFRGTAEEETPAGSCTLAGLPQGRRNDVRLKKTTDINNPGR
                     NVMRERLKSVTTALLAAGAVAL"
     gene            complement(229..1749)
                     /gene="adh"
                     /locus_tag="VNR65_18335"
     CDS             complement(229..1749)
                     /gene="adh"
                     /locus_tag="VNR65_18335"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HWJ20680.1"
                     /translation="MLQGAINAIQAKVVWRTRYDNFIGGGWVPPMGGEYFTNISPITG
                     KPIAEIARGRAADIHLALDAAHRAKGGWGRTSPAERSAILNKIADRMEQNLNILALSE
                     TIDNGKPIRETTAADIPLAIDHFRYFASCIRAQEGSLSEIDHDTVAYHYHEPLGVVGQ
                     IIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPMSILVLIDLIKDLLPAGVLNVVNG
                     FGVEAGKPLAQSNRIAKIAFTGETSTGRLIMQYASENLIPVTLELGGKSPNIFFADVM
                     AADDDFFDKALEGFAMFALNQGEVCTCPSRALVQRSIYDRFMERAIARVGKVKQGHPL
                     DPTTMIGAQASNDQLEKILSYIDIGKKEGAKVLCGGERNVTSDETREGYYVKPTVLEG
                     HNKMRIFQEEIFGPVLCVTPFDTEEEALHIANDTLYGLGAGVWTRDGNRAYRFGRAIQ
                     AGRVWTNCYHLYPAHAAFGGYKQSGIGRENHSMMLDHYQQTKNLLVSYSPKALGFF"
     gene            1992..>2758
                     /locus_tag="VNR65_18340"
     CDS             1992..>2758
                     /locus_tag="VNR65_18340"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HWJ20681.1"
                     /translation="MSVGLAKRKSYACGVQVAWSEEPSVDAAVGSIAVALDRSEIGQL
                     LVFFSPSRVSSTLSRALSHEFAGIPVAGCSTSGQISPDCFSEVGILAMAFPKKGFRVV
                     SKVLRDIHGLSIERAIEVVRDLREQLDQASAGQKRNSFALSFIDGLSNSEEMIVSALG
                     WALNDIPLVGGSAGDNLLFSRTALLHNGEVHHEAALLLLIETDYLVRTFKHDNFEPTD
                     QKLVVTASDSESRTVLELNAGCAAREYAAAAGLDDGSL"
BASE COUNT          527 a          780 c          844 g          607 t
ORIGIN      
        1 caaagccacg gctcccgctg caagcagcgc ggtcgtgacc gatttcaacc tttctctcat
       61 gacgtttctt cccgggttgt tgatgtcagt tgtctttttt agccggacgt cattgcgccg
      121 accttgagga agcccagcca gggtgcagga accggctggg gtctcttctt ccgctgtgcc
      181 gcggaattct tttaccacga aagagcagag agcattcaga gccgaggctc aaaagaagcc
      241 aagagctttc gggctgtagc tcaccaagag gttcttggtc tgctgataat gatcgagcat
      301 catgctgtgg ttctcacggc caatgcccga ctgcttgtag ccaccgaatg cggcgtgagc
      361 gggatagagg tggtagcagt tcgtccagac gcggccagcc tgaatggcgc ggccaaagcg
      421 ataagcgcga tttccgtcac gggtccaaac gccggcgcca agcccgtaga gcgtgtcgtt
      481 cgcgatgtgg agcgcttcct cttcggtgtc gaacggggtc acgcacaaaa ccgggccaaa
      541 aatctcctcc tggaagatcc gcatcttgtt atgaccttcg agcacggtcg gcttgacgta
      601 atagccttcg cgcgtctcgt cggaggtgac attgcgctcg ccgccgcaga gcaccttggc
      661 gccttccttc ttgccgatgt cgatgtacga caagattttc tcgagctgat cgttggacgc
      721 ctgcgctccg atcatcgtcg ttggatcgag cggatggcct tgcttgactt tccccacgcg
      781 cgcaatcgcc cgttccatga aacggtcgta gatggagcgc tgaaccaggg cgcggctcgg
      841 gcatgtgcag acctcgcctt ggttcaaggc gaacatggcg aagccctcaa gcgccttgtc
      901 gaagaaatcg tcgtctgccg ccatcacatc ggcgaagaag atgttcggag acttaccgcc
      961 gagttccaac gtcaccggaa tgaggttttc cgaggcatac tgcatgatca ggcggccggt
     1021 cgacgtctcg cccgtaaacg cgatcttggc gatgcgattg ctttgcgcga gcggcttgcc
     1081 cgcctcgacg ccgaagccgt tcacgacatt gaggacgccc gcagggagga gatccttgat
     1141 gaggtcgatc aggaccaaga tgctcatcgg cgtctgctcg gcgggcttca gcaccacgca
     1201 gtttccggct gcgagcgcag gcgcgagctt ccagaccgcc atcaggatcg gaaagttcca
     1261 cgggatgatc tgcccgacga cgccgagcgg ctcgtggtag tggtatgcaa ccgtatcgtg
     1321 atcgatttcg gagagcgagc cttcctgagc gcggatgcag gaggcgaaat accggaaatg
     1381 gtcgatagcg agcggaatgt cggccgccgt cgtctcgcgg atcggcttgc cgttgtcgat
     1441 cgtctcggaa agggcgagaa tgttgaggtt ctgctccatc cgatcggcga tcttgttcag
     1501 gatggcggag cgctctgcgg gagaggtacg accccatccg ccttttgccc ggtgtgcggc
     1561 atcgagagca aggtgaatgt ccgctgcccg gccgcgtgcg atctcggcga tgggcttgcc
     1621 ggtaatcggg ctgatattgg tgaagtattc tccgcccatt ggaggcaccc agcctccgcc
     1681 aataaagttg tcgtagcggg tgcgccagac gacttttgcc tggatggcat tgattgcccc
     1741 ttgaagcata aacgtgtcct tccctgaatt cgatctttgg ctattggcta gccaaaagca
     1801 ggcactgggc ggtatcgccg ccgggtggcc tttatcttcg ttccgtcgcc tatcgcaggc
     1861 ttggccaatc gtagtttgac aagcatcaaa accggtattg tactttcggt cgcgatcgcc
     1921 tttgcggtat ggtcaaatcg aaaaccggcg ccggaagaag cgccgcgatg gaggagagtg
     1981 ctttggagag cgtgtcggtc ggccttgcca agcgcaaatc ttatgcttgc ggcgtgcaag
     2041 tggcgtggag cgaagagcct tccgtcgacg cagcggtagg ttccattgcg gtggcgctcg
     2101 atcgttctga aatcggtcag cttctcgtct tcttttcccc ttcccgtgtg agcagcaccc
     2161 tgtcgcgggc cctttcacat gaattcgccg gcatcccggt tgcaggctgc agcacgtcgg
     2221 gacagatctc cccggattgc tttagcgagg ttgggatcct ggcgatggcc tttccaaaga
     2281 aagggttccg ggtcgtcagc aaagtgttgc gagacattca cgggttgtcg atcgaacgcg
     2341 caatcgaagt tgtccgcgat cttcgagagc agctcgatca ggcgtccgca ggccaaaagc
     2401 gcaacagttt tgctctgagc ttcatcgatg ggctgtctaa tagcgaggaa atgatcgtgt
     2461 ccgccctcgg ctgggcgctc aacgatattc cgctcgtcgg cggctcggcc ggagataatc
     2521 tccttttttc gaggacggca ctccttcata acggggaggt ccatcatgaa gcagcgcttc
     2581 tgcttcttat cgaaaccgat tacctcgtgc gcactttcaa gcacgacaat ttcgagccta
     2641 cggatcaaaa gctcgttgtg acagcttcgg acagcgagag caggacggtg cttgagttga
     2701 acgcaggatg cgcggcccgg gaatacgccg cggccgccgg gctcgacgac ggaagcct
//
LOCUS       DATMGX010001121         2067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_208875, whole genome shotgun sequence.
ACCESSION   DATMGX010001121 DATMGX010000000
VERSION     DATMGX010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208875"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..954
                     /locus_tag="VNR65_18345"
     CDS             118..954
                     /locus_tag="VNR65_18345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWJ20682.1"
                     /translation="MNLRMWVSRASSTAHVSWHVLKNFLLLPYYGFRDRGFLHQQMRA
                     FVANVINLEKEYQTRVERVNWTGLQKLVGGTDPSLRFRTGARYGNVSFEESLVLAYLV
                     ESLRPRQIFEIGTFDGFSTYHLAMNGAADAEVYTLNLPVEDEGPASQLARGADSEYFG
                     DNKTHQELKRRGVGSVYRACPAAGKVHQLFGDSLTFNFTPYREVIDLVFIDGGHSLEC
                     ITKDTESALAMLSDRGVIVWHDFNVQHRDVFRFLREFAKTRKTYWIAGTRLALFGPCL
                     TR"
     gene            1180..>2067
                     /locus_tag="VNR65_18350"
     CDS             1180..>2067
                     /locus_tag="VNR65_18350"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWJ20683.1"
                     /translation="METNELGQADYERLYRSLRLIRRAEEEIARIYPSDKIKSPVHLS
                     IGQEAVSVGVCDVLRPDDVVAPTYRGHAAFLAKGAPLRGLMAELYGKATGVAGGKGGS
                     MHIIDTKHNVLGASAVVGTTIPIAIGYALTFKRMRSDRVAVTFFGDGATEEGVFAESL
                     NFASLHKLPVLFVCENNYYAIHTPITRRWATQQLCERVATYGIPAHQIDDGDVLKLRA
                     LTADSLDALRRGDGPVFIECRTYRWREHVGPSEDYTQGYRARSDLEPWIEGDQMESLG
                     ARLVPNRRASIDAEIEREIA"
BASE COUNT          407 a          628 c          602 g          430 t
ORIGIN      
        1 cggctctgag ccagtgcgca tcggcggcgt gcgcaagatg atgccctttc gcaccggctc
       61 cgcggtgctt ttccagattg cacaagagtt cctcactcgc cgtccggagc gggccccatg
      121 aatctgcgga tgtgggtttc gcgtgcgtca tccacggcgc acgtttcttg gcacgtcctc
      181 aagaacttcc tcttattgcc atattacggc tttcgcgaca gaggcttcct ccatcagcag
      241 atgcgcgcgt ttgtcgccaa tgtcatcaat ctcgaaaagg aatatcagac acgcgtcgag
      301 cgggtgaact ggacgggctt gcagaagctt gtcggcggga ccgatccatc tctgcgtttc
      361 cgcaccggcg cccgctatgg caacgttagc ttcgaggaga gcctggtcct cgcttatctc
      421 gtcgaatcgc ttcggccgcg gcagatcttc gaaatcggaa cgtttgacgg tttttcgacc
      481 taccatctcg cgatgaacgg cgccgccgac gccgaggtgt acacgctgaa cctcccggtc
      541 gaggacgagg gccccgccag ccagctcgcg cgaggggccg acagcgagta cttcggcgac
      601 aacaaaacgc accaggaact caagcggcgc ggcgtcggat ccgtttaccg cgcttgtcca
      661 gcggccggta aggtccacca actcttcggc gactcgctaa ccttcaattt tacgccttac
      721 cgtgaagtga ttgatctcgt tttcatcgac ggaggccact cgctcgaatg catcacaaag
      781 gatacagaga gcgcgctcgc gatgctctcc gaccgcggcg tgattgtttg gcatgacttc
      841 aatgtccagc atcgcgacgt attccgattt ctgcgggaat tcgcaaagac gcggaagacc
      901 tattggatcg cgggcacgcg cttggccctg tttggacctt gtctaactag gtgattgaaa
      961 tcattaatga actagtcgtt ggtgacaggc attctgaaaa ccatatcaaa tactgatcga
     1021 acggctatcc gttgaacatg actgcgattt ttgtttgcat aattacggga attggtcttt
     1081 gtcgtcggca cttaactggc gcaccgacgc ggacttcgcc cttccagatt ttgcgcggtt
     1141 gtcggttggt attgccgtta gatgaggggc atcctttcaa tggagactaa cgagctcgga
     1201 caggccgact acgagcgact ctaccgctcg ctgcgcttga tccgtcgagc cgaagaagaa
     1261 atcgcacgca tctatccgtc cgacaagatc aagagtcccg ttcatctctc gatcgggcaa
     1321 gaggcggtat cggtcggcgt ttgcgacgtt ctgcgtccgg acgacgtggt cgcgccgacc
     1381 taccgcggac acgccgcctt tctcgccaag ggagcaccgc tgcgcggcct gatggcggag
     1441 ctctacggca aggcaaccgg cgtcgccggc ggcaaaggcg gttcgatgca catcatcgat
     1501 acaaaacaca acgtgctggg cgcttccgcc gtcgtcggca cgaccattcc gatcgcgatt
     1561 ggttatgcgc tcacattcaa gcgcatgcgc agcgatcgtg tcgcagtgac gttcttcggc
     1621 gatggcgcga ccgaggaagg cgtttttgcc gagagcctca acttcgcgtc gctgcacaag
     1681 ctgccggtgc tgttcgtgtg tgagaataat tactacgcca tccacacgcc gatcacgcgg
     1741 cgctgggcga cgcaacaact gtgcgaacgg gtcgcgacct acggcattcc cgcccatcag
     1801 atcgacgacg gcgacgtgct caagctgcgc gcgctcaccg ccgactcgct cgacgcgctg
     1861 cgccgcggcg atggcccggt gttcatcgaa tgccgtacct atcgctggcg cgagcacgtc
     1921 ggcccgagcg aggactatac gcagggctat cgtgcacgct cggatctgga gccgtggatc
     1981 gagggcgacc aaatggaaag cctcggcgcg cgactcgttc caaaccgccg cgcttcaatc
     2041 gacgcagaaa tcgaacgcga gatcgcg
//
LOCUS       DATMGX010001122         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_245420, whole genome shotgun sequence.
ACCESSION   DATMGX010001122 DATMGX010000000
VERSION     DATMGX010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245420"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..513)
                     /locus_tag="VNR65_18355"
     CDS             complement(118..513)
                     /locus_tag="VNR65_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20684.1"
                     /translation="MNLHEQELVESFVQRARRERVRLGLASPKKRRKFGDEFAHHGTY
                     ILAPECLRSIKPRQQNPDSICAILHALGAPDTCYLISEDTNLDGKEIKLLAALKQVVG
                     YGMGTVISCLPGRLGYFEGELRERYILQK"
     gene            complement(543..959)
                     /locus_tag="VNR65_18360"
     CDS             complement(543..959)
                     /locus_tag="VNR65_18360"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWJ20685.1"
                     /translation="MTRLWMLDTNTVSYIVRGKSPAARTKLYRLQGDEIGCISVITEA
                     ELRYGTSKQAPGSVRLAALNTLFDKLRILPWTSEEALVYGDLRAKLERAGKVLGNLDM
                     LIAAHAISANATLVTRDKAFVQVEDLRPPVNWAIDL"
     gene            complement(956..1207)
                     /gene="vapB"
                     /locus_tag="VNR65_18365"
     CDS             complement(956..1207)
                     /gene="vapB"
                     /locus_tag="VNR65_18365"
                     /inference="COORDINATES: protein motif:HMM:NF040493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWJ20686.1"
                     /translation="MVPISKAKVFYSGRSQAVRIPAEFRFTTDEVYVRRDPQSGDLIL
                     SESPVKTWAEVFKALDDAKFPDDFLEDRDQRPPQKRDNL"
     gene            complement(1315..>2249)
                     /locus_tag="VNR65_18370"
     CDS             complement(1315..>2249)
                     /locus_tag="VNR65_18370"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWJ20687.1"
                     /translation="VNITSRVMTFDFFYGLVPQEGQGSGFVIDKEGHILTNYHVIADA
                     RQVEVTLHDRKKYKATIVGTDKSHDLAIVQIKAPNLQPMTLGDSTNLQVGQKVYAIGN
                     PFGLAGTLTRGIVSSIRQVQEPDGLVIDEAIQTDAAINPGNSGGPLLNWHGEVIGINT
                     MIASSVGQSAGIGFAIPINTAKAVVNDLVTLGRVRRPALGVRTIPIDPEIANELGLAA
                     DYGLLIVQAVPGGSADRAGLRGGSERAYLGNTPIMIGGDLIVAVDGEKIESQQKLAQM
                     MNKHRAGDTVKVTIFRGKKQVEVSVALGEARDQV"
BASE COUNT          454 a          592 c          697 g          506 t
ORIGIN      
        1 tgaaggggtc gccaggaaag ctgagggcga gttagcggac ggcggtcata agccgggaga
       61 taggatacaa cggtttgctg ggagaaggca cgccctagaa gagcagtttg cgtatggcta
      121 tttctgcagt atgtagcgct ccctcaattc accttcgaaa tatccaagcc gtccgggaag
      181 gcaggaaatc acagttccca tgccatagcc gacaacctgc ttcagggcgg ccaacaactt
      241 tatttccttg ccgtcgagat tggtgtcttc agagataagg tagcaagtat cgggagcacc
      301 gagtgcgtga aggatcgcgc agattgaatc tgggttctgt tgacggggct ttatgcttct
      361 aaggcattcg ggcgcaagaa tgtaggttcc gtggtgagca aactcgtcgc cgaacttgcg
      421 gcgctttttg ggactggcga gaccgagtcg cacacgctcg cggcgcgcac gttggacaaa
      481 agactccacg agctcctgct catgtaggtt catatggcta tccaaggaga agtcaaccga
      541 gcctataaat cgatcgccca gttcacgggc ggacgcagat cttcgacctg cacaaacgct
      601 ttatcccggg taaccagagt tgcgtttgca ctgatggcat gcgcggcgat tagcatatcc
      661 agattgccca ggacttttcc ggcccgttcc agtttggccc gcaggtctcc ataaacaaga
      721 gcttcctcgc tggtccaggg aagaattcgc agcttgtcga acaaggtatt cagcgcggcg
      781 agacgcaccg atcctggggc ctgcttggac gtaccgtaac ggagttcggc ttcggtaatc
      841 accgaaatgc agccgatttc atcgccttga agtctgtaca gtttggttct tgccgcaggc
      901 gacttcccac ggacaatgta gctcacggtg ttggtatcga gcatccagag tcgagtcata
      961 gattgtcgcg tttttgaggc ggtctctggt ctcgatcttc caggaaatcg tcagggaact
     1021 tggcgtcatc gagagcctta aagacttctg cccaagtttt gacaggggat tcggagagaa
     1081 tcagatcccc gctctgagga tcgcggcgga cgtagacctc gtccgtcgtg aagcgaaact
     1141 cggcgggaat ccgaacggcc tggctcctgc cgctgtagaa gacctttgct ttagaaatcg
     1201 gcaccatagc ggtccttttc tgctatatat catgctatat agcaagaagg attgcaagag
     1261 cgcattttgc cgacggccaa ctgcacgcgg gcgagggcgc ccgcgccaca cgagctagac
     1321 ttggtctcta gcttcaccca gcgccaccga tacttccacc tgtttcttgc cgcggaagat
     1381 ggttactttc acggtatccc cagcgcggtg tttgttcatc atctgggcga gtttttgctg
     1441 gctttcgatt ttttcgccgt cgacggcaac gatcaaatct ccgccgatca tgatgggagt
     1501 gttgccgagg taggcgcgct cggagccgcc gcgcaatccg gcgcgatcgg cggagcctcc
     1561 gggaacagcc tgcacgatga gcagtccgta gtcggcggcg aggccaagct cgttggcgat
     1621 ttcgggatcg atggggatgg tgcgcacgcc gagcgcggga cggcgcacgc gtccgagcgt
     1681 gaccaggtcg ttgaccacgg ctttggcggt gttgatcggg atggcgaagc cgatgccggc
     1741 gctttgtcca acgctcgagg cgatcatcgt attgatgccg ataacctctc cgtgccagtt
     1801 gaggagcggg cctccggagt tgccggggtt gatggcggcg tcggtctgga tcgcttcgtc
     1861 aatgaccaaa ccgtcgggtt cctgcacctg gcggatggaa ctgacgatgc cgcgcgtgag
     1921 agttccggcg agaccgaagg gattgccgat cgcatagacc ttctggccga cctgcagatt
     1981 ggtcgagtcg ccgagggtca tcggctgcag gttgggcgcc ttgatctgga ctatggccag
     2041 gtcatgagac ttgtcggtgc cgactatggt ggccttatat ttcttgcggt cgtgcagcgt
     2101 gacttctacc tgacgcgcat cggcgatgac gtgatagttg gtgaggatgt gtccttcttt
     2161 gtcgataacg aaaccggagc cttggccttc ctgcgggacg aggccataga agaagtcgaa
     2221 ggtcatgacg cgagaggtga tgttgacga
//
LOCUS       DATMGX010001123         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_94158, whole genome shotgun sequence.
ACCESSION   DATMGX010001123 DATMGX010000000
VERSION     DATMGX010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94158"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VNR65_18375"
     CDS             complement(<1..555)
                     /locus_tag="VNR65_18375"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HWJ20688.1"
                     /translation="MPTQQKGRLREEEATLSDTDLLTRAGELVAGGFPIVGIGASAGG
                     LDAFKAFLAAMPPGTGMAFVLVQHLDPIHVSLMAGLLSGHTAMPVTQAVEGVSIEPNR
                     VYLIPPGVSLAVAAGKLHLFELTERHGSRTAFDVFLRSLAEDCGERAIGIVLSGTGSD
                     GSEGLKAVKSKGGFVIVQEPSEAAF"
     gene            complement(828..1541)
                     /locus_tag="VNR65_18380"
     CDS             complement(828..1541)
                     /locus_tag="VNR65_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWJ20689.1"
                     /translation="MRILNRIMGFDHQLALTDRQREYSKLIQASSKSLLSLIGGVHDL
                     KKIARGKVGALPFKLNVLAKSPECGPAARRAIDNDACTYKVLVAEDHQINVTLILAVL
                     DVAGCETELAENGLQVLAKLEQADFDLIIMDSQMPMLDGLEATKRIRSRSDWKSLIPI
                     LSLTADASEGAEERVLSAGANVYMTKPFNFDCLIASVKSLAWRGRALCRDPVMEGVVN
                     RAKEMVAPPLSVLPRHAGA"
     gene            complement(2065..>2365)
                     /locus_tag="VNR65_18385"
     CDS             complement(2065..>2365)
                     /locus_tag="VNR65_18385"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HWJ20690.1"
                     /translation="DELGMSEITTARPVQAALTSAAMFSTGAAMPLLMVVLSPASALI
                     PVVSAASLGFLALLGAIGARTGGANIVRATVRVAFWGALAMAITAGIGKLFGTIV"
BASE COUNT          532 a          684 c          638 g          511 t
ORIGIN      
        1 gaatgccgcc tcggaaggct cctggacgat gacgaacccg cccttactct taacggcctt
       61 cagaccctcg ctgccgtccg agccggtgcc tgacaagacg atgccgattg cgcgttcgcc
      121 gcaatcctcg gcaagcgacc tcaggaagac atcgaaagct gtgcgacttc catgacgctc
      181 cgtcagctcg aacagatgca atttccctgc agcgaccgca agcgagaccc ccggagggat
      241 caggtagacg cggtttggct cgatggacac gccttcaacc gcttgcgtga caggcatggc
      301 cgtatgtccc gacagaagcc cggccatcaa gctgacgtgg ataggatcaa gatgctgaac
      361 gaggacgaag gccatgcctg tgccgggtgg cattgcggcc aggaacgcct tgaaggcgtc
      421 gaggccgccg gcggatgcgc cgatgcccac aattggaaat ccgcctgcga caagctcccc
      481 cgcacgagtg aggagatctg tatcggaaag cgttgcctct tcttctctga ggcggccctt
      541 ctgttgcgtt ggcatgcagg tttcctccgc tccaggctga gtagacctgc aacaagagct
      601 atgcatatca tagctcgcca aaattcgtgt gccgatttta atttgcaaaa ttgcggaccc
      661 gacctcggcg gacaataccg actagtcatc ccacgaagat aggctcggaa agtacctagc
      721 ccgtttcgct aaaatcgcat catgatcttt ctacaccagg aaggtgggta ggtacgcctg
      781 aaaggccgtc aagctgccgg acgtaaacgt ggctaaaccg gatgccgcta ggcgccggca
      841 tgccgcggta ggacagatag tgggggtgca accatttctt ttgcccggtt gacgacacct
      901 tccatcaccg ggtcccggca taacgcacgt ccacgccaag caagggattt gacggaagca
      961 atcaagcaat cgaagttgaa aggctttgtc atatagacgt tggcgcccgc ggataagacc
     1021 ctttcttctg caccttcgct ggcatccgcg gtgagagaca agatcgggat gaggcttttc
     1081 caatcggagc ggctccggat tctcttcgtc gcctccagcc catccagcat aggcatctgg
     1141 ctatccataa tgatgagatc gaagtccgcc tgttccagct tcgccaatac ttgcaggcca
     1201 ttctccgcaa gctccgtttc gcatccggcg acatcaagaa cagctaagat aagtgtcaca
     1261 ttgatctgat gatcttccgc tacgagaacc ttataggtgc aagcgtcgtt atcaatcgcg
     1321 cgcctcgccg ccggcccgca ttcgggactt ttggcgagga cgttgagttt gaacgggagt
     1381 gctccgactt tccctctcgc gatcttctta agatcatgga caccgccaat cagcgacagg
     1441 agagatttgg atgaagcttg aataagcttg gaatactccc tctgccgatc cgtgagtgct
     1501 agctgatgat cgaagcccat gatcctgttc aggattctca cctcatggcg cacacttttc
     1561 aggaattggc ttttcgcccg atcggtcgct ttcgcacgag cccgcgctcg ctcttgctgc
     1621 gcttgacact gtttcaatgt atgaaccccc gatttcggct ttttcgctcg atgtgcagtg
     1681 ctccagtgcc gctggaactt tgctggcgaa gctatgctag gcaatggtga gccaatccga
     1741 tagactcgga aaacactcag tgcaattatt tacttggcat cgtccatggc tattaacgaa
     1801 ataagaatat agccaccttt tggttcgccc ttagttgtca cattataagt atttgcaatc
     1861 acacaaactg actctcagtt cacgcccgat ggccgcgccg cgatcaattt gaagactgta
     1921 tcgtaaagat ttgaaattat ccgcagcgtg gtttcacggg tatccacctt gtgattcaag
     1981 gggtaaacct ccggaggtct ttcgcgtaac ggcaatcgac aaagccgatc gcgtcggcct
     2041 aaacctcccg cacgtcgccg atcttcagac aatcgtgccg aagagtttcc cgatacccgc
     2101 agtgatagcc atggctagcg cgccccaaaa tgccactcgg actgtagcgc gcacgatgtt
     2161 ggcgccgcca gttctggctc cgatcgctcc gagaagcgca aggaagccca aggacgcggc
     2221 ggaaacaacg ggaataagcg cggaggcagg cgagaggacg accataagga gaggcatggc
     2281 ggcccctgtc gaaaacatgg ctgccgatgt cagggctgct tgcaccggac gcgcggtcgt
     2341 aatttccgac atgcccagct cgtcg
//
LOCUS       DATMGX010001124         2465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_246354, whole genome shotgun sequence.
ACCESSION   DATMGX010001124 DATMGX010000000
VERSION     DATMGX010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246354"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(494..1045)
                     /locus_tag="VNR65_18390"
     CDS             complement(494..1045)
                     /locus_tag="VNR65_18390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWJ20691.1"
                     /translation="MSSMFDPKQLPPLDRTDDWLRIVRAFLSALSVRKKEAFNRRVSV
                     GDLLTERGDNAAMCGFGEGTTMYDNVLVLGEVKVGRKTWIGPNCILDGSGGSLEIGDW
                     CSISAGVQIYTHNTVKRSISLGVEPIEYAPTKIGHGVYIGPNAVVQMGVSIGDKAIIG
                     ANSFVNRDVPSNSKAFGSPAKIQ"
     gene            complement(2069..2332)
                     /locus_tag="VNR65_18395"
     CDS             complement(2069..2332)
                     /locus_tag="VNR65_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20692.1"
                     /translation="MTLENSPSKVVAADQPFSHLFSTEAVTSTESPACATPLTIKDAP
                     GSVWRRRYITVRVEIVRPSHTTSQSEHAVRIAYDLSARSPSSL"
BASE COUNT          561 a          694 c          653 g          557 t
ORIGIN      
        1 cccgagaggg cgtcgagttc atcgacgaaa acggcggcgg cgccggtgtg cgtttgcgaa
       61 agcctagcgg caaaggaccc aagaaaaccg ggtgacttgg tcacctaacg cgacgacaat
      121 accatctgca aaacgaaccc aatattgccg aataaataca aatacttgaa aatttccggg
      181 cgtgaaattc acggcggatt gcaaactctg agccgcatcg aaatgggtgg aaaagcctgt
      241 gggccgaaat aaaatctgac ttcgctactt taatgccgag cggtgtagat gcgtccggat
      301 tagttgggga gcggtcatcg gaaaccaatt tgattccccg gagccctgat gacgttacag
      361 gggcagaccg acgcgttggc agctttgggc atgatcacga cgcccccaat tgcaccgcgg
      421 tccgagacta aattcatggc tctgatcttg cgtccgccca taatccatct gcggaggcgc
      481 gtagatacgc acgctactga attttggcag gcgaaccaaa agccttcgaa ttggagggaa
      541 cgtcgcggtt tacgaaggaa tttgcaccta taatcgcttt gtctccaatc gaaaccccca
      601 tctgaaccac agcattcggt ccaatataga cgccatgacc aattttcgtc ggtgcatatt
      661 cgatcggctc aacaccaaga gaaattgacc gcttaacggt attgtgagtg tagatttgta
      721 cacccgcaga aatcgaacac cagtccccga tttccagact cccgcccgag ccatccaaaa
      781 tgcaatttgg tccgatccac gttttgcgac caactttcac ctcaccaagc acgagcacgt
      841 tgtcgtacat tgtcgtgcct tctccaaatc cacacattgc ggcgttgtcg ccgcgctcgg
      901 tcagaagatc tccaacggaa acgcgacgat tgaaggcttc tttctttcgg acggatagtg
      961 cggagagaaa tgccctaacg atccgaagcc aatcgtccgt gcgatccaac gggggcagtt
     1021 gcttagggtc gaacatcgaa gacatttgca atcaccatcg gctaaaaggt ccgagcaatg
     1081 tagcagtcga aacactcctc gcaagtccga accaacgcgc gcttgcctaa atcagaattc
     1141 tcaatgttca cgctttacag gaattttccc aagatagtct gactcagtca gacgcccatc
     1201 caccacatta acggtaatag tttggcggac gatgttaatc aggggcggac gccggctctg
     1261 gtcctttaag cgacaatttg gtaccgttta ccagcctcga gagatttcaa ccggcgatgc
     1321 tacaaagcca aacactctgt ctatttgttc tggtactgat gttaaagtgg gatgggagga
     1381 cccgatatgc gattgatcac ttccgcgctt ctcgcgttgg gattgtttgt tggtagttcc
     1441 tttgttcaag cgtcgccgct gatttcgtca gcaggtaacc ttgctcattt caacagtgca
     1501 tttgagagcg tccgcgtggt ttgcggcggc tgcggctacc ggccacaaac gcgttaccgg
     1561 cacgtccgac gccacaggca acaatatgca ttcccgcctc acacctattt cgattatttc
     1621 gggccattga acccagcgct acgattttac ggatgtccct gtcggtcgta aggtaccgcc
     1681 atagagaact tgctcggaag cacgttccga ccgtctacag ctcggttctc ggccggcggc
     1741 cactccgccc tgttccaggc cgacatgttc atctggcaga atgcgagcgc tgcttatttc
     1801 ccagcgactg gcctatggtt ggctggacat caccacaaac cgcaccgtga ccgtcgcgag
     1861 catcgatcag ctgcgcgcgc agttcaacgc caatgcatgg tccgggcgtg ccgaaggcgg
     1921 ctatcgtttt gtcgttcctt gggttggcgg catcggtatc gcgccctacg ccgcgggtca
     1981 gttcactacc tttgatcttc ctgcttatgc cgagcagggt acgattcgta gcaacagatt
     2041 tgcgttagtt agcgcaaaga gcgttacctc acagcgagct tggactgcgc gccgacaaat
     2101 catacgcgat gcggacggca tgctcactct gcgaggtcgt gtggcttggg cgcacgattt
     2161 caaccctgac cgtaatgtag cggcggcgcc agacgctgcc gggcgcatcc tttatcgtca
     2221 gcggagtcgc gcaggcgggc gactccgtgc tcgtgaccgc ctcagtcgag aaaagatggc
     2281 tgaacggttg gtcagctgcc accaccttcg agggcgagtt ctccagcgtc actgcgagct
     2341 gcgccggcaa gggagtggtg aggtattcct ggtaaggtcg cgacctccgt ttttggctgc
     2401 caatagccga cgcaaatttt aaaggaccat tcagagcccg tcagacggct tagtcatgcc
     2461 cttcc
//
LOCUS       DATMGX010001125         3484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_348738, whole genome shotgun sequence.
ACCESSION   DATMGX010001125 DATMGX010000000
VERSION     DATMGX010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348738"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1155
                     /locus_tag="VNR65_18400"
     CDS             277..1155
                     /locus_tag="VNR65_18400"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWJ20693.1"
                     /translation="MTDKAISPLRRRMIEDMTIRKFASKTQHDYVQRVKHFAAFLRRS
                     PDTASFEDVRRYQLHLATSGASVPTLNQSVSTLRFFFKITLGRADIVNHTQFIHVPRK
                     LPVILSPEEVARFLDAAPGLKYKAALSVAYGAGLRVSEVVALKVSDIDSKRMVIRIEQ
                     GKGHKDRYVMLSPHLLELLRAWYNAARPQGWLFPGMNPVNPMSARQLRRACDTAAQMA
                     EIGKQVSPHTLRHSFATHLLEQNTDIRVIQVLLGHAKLDTTALYTRVATKIIREIMSP
                     LDRITSKLKEGVEPPA"
     gene            1164..1313
                     /locus_tag="VNR65_18405"
                     /pseudo
     CDS             1164..1313
                     /locus_tag="VNR65_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            1851..2381
                     /locus_tag="VNR65_18410"
     CDS             1851..2381
                     /locus_tag="VNR65_18410"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20694.1"
                     /translation="MSDTVRSLNTSGLAPKKQIQCWSDTLTDLCGHFDIDPLEASSFE
                     GRINYTTVSKLKLCQIEASQHRLAHTASRAKLGGHPFVKIHFQTYGISHFEQGGRRIE
                     LMPGDCLAYDVSCPHTIVSPTLTRHEVVIVPKELLQERGFRLAKMTACKFSARTGTGR
                     IAYNFVHAAFDEATKL"
     gene            2400..2825
                     /locus_tag="VNR65_18415"
     CDS             2400..2825
                     /locus_tag="VNR65_18415"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWJ20695.1"
                     /translation="MADSLIDLLLLPLREPDAMFNRVGPEAMNVQARFFIREHLRDPD
                     LSIDQISAALNCTKRYLHMLFSDRGTTISDYIQNARLQNCRQELETQAGKPITDVAFS
                     WGFSSSSHFSRVFRKHFGVIPSAIHKAQHGGPYPDLPCE"
     gene            complement(3080..3160)
                     /gene="pqqA"
                     /locus_tag="VNR65_18420"
     CDS             complement(3080..3160)
                     /gene="pqqA"
                     /locus_tag="VNR65_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HWJ20696.1"
                     /translation="MTWKTPKVVEVPVGMEINMYACAARK"
BASE COUNT          720 a         1087 c          991 g          686 t
ORIGIN      
        1 aagcagaggt tcggcgcctc gccccgtgaa tggcgcgagc acccgacgcg atagcacgtg
       61 aagccaaaaa tttggcgtgg tcagcggatg tccgcttggg tgcacactac ggactcagat
      121 cggacatcgc gccatgtccg aaaagtgcca caggggacta aaccgctcgc gcggtagttc
      181 gccgcggcgg gcgacggagc caacacgata gcgcgccggg gcgtcctgtc ttgagatgag
      241 gggttcgcaa ccttcaccaa gacaggagca tcccccatga ccgacaaggc cataagcccg
      301 ctgcggcggc gcatgatcga agacatgacg atccgcaagt tcgcgtcgaa gacccaacat
      361 gactacgtgc agagggtcaa gcacttcgct gctttcctcc gccgctcgcc cgacaccgcg
      421 agcttcgaag acgtgcgccg ctatcagctt catctggcga cgagtggcgc cagcgttcct
      481 accctcaacc aaagcgtctc gacactgcgg ttctttttca agatcacgct tgggcgcgcc
      541 gacatcgtga accataccca gttcatccac gtgccacgca agcttccggt catcctgagc
      601 ccagaggaag tcgcgcgttt tctcgatgcg gcgccggggc tcaaatacaa ggcggcattg
      661 agtgtagcct atggagccgg attgcgagtg tccgaggtgg ttgcgctgaa ggtatccgac
      721 atcgacagca agcgcatggt gatccggatc gaacagggca agggccacaa agaccgctac
      781 gttatgcttt caccccatct gctcgagctg ttgcgcgcct ggtacaacgc agcacgccca
      841 caggggtggc tgtttccggg aatgaacccg gtcaacccga tgtcggcccg ccagctcagg
      901 cgcgcctgcg ataccgcggc ccagatggcc gagatcggca agcaggtgtc accgcacacc
      961 ctgcgacaca gctttgcgac gcaccttctg gagcagaaca ccgatatcag ggtgatccag
     1021 gtcttgttgg gacatgccaa gctcgacacc accgcgctct atacgcgtgt cgccaccaag
     1081 atcatccggg agatcatgag cccgctcgat cgcattacaa gcaagctcaa ggagggcgtc
     1141 gaaccgcccg cctgacgcgc gtcgtgtcgc gtccatcgct gcaggtcgcg gatatcttcc
     1201 gtggccacgg cccggcatgg cgccgagcca atgccggcca tatgagcctc ggcctactca
     1261 aggtgatgtc ggcgatcgag aactgccgca cggcggcgct cggcgggagc gtcttgagat
     1321 tttctatgtc ctcgcactga gagagcaagc gtggtgaccc agttgcgtcc acatcgcact
     1381 cctcgcggcc atgcgatgga cgtctagtcg cccgccgcgc gtcggccgaa gcttgcacct
     1441 catctgaggg tcgcgatcat tcctgttcga ggacatccac aatcgacgcc acgcttgcca
     1501 cgagccaagc ttattggaga cgatcatccc tacccggttc ggaaacagga cacttgccca
     1561 agcgggaagc gcgtttgcgc gaaagcgaaa cacaatgaac tttttagcgg cgcccgtgat
     1621 ctccgcaggc ggagcgcttc catgctgcga tgcgtcagcc gtaacgcgcg accccttggt
     1681 tgaggttctc cttgacggcg ctgaaactaa gtcggaggat tatcaaaggg atgtcagtag
     1741 gcgtgctgtt cgaaccgacc gccttaaatt gaggtaaaag tcgctccatc atgacccgaa
     1801 gcctctcgcg gcaagcagtc atgttttaat ctcgaatcgg tggctggata atgtccgaca
     1861 cggtccgctc acttaacact tcaggattgg cgccgaagaa gcagatccaa tgttggtcag
     1921 atacactgac cgatctttgc ggccatttcg acatcgatcc gctggaggct tcgtcattcg
     1981 aaggccggat caactacacg acggtttcga agctgaagct ctgtcagatc gaggcgagcc
     2041 agcaccggct tgcgcatacc gcctcgcgcg caaaactggg cggtcatccg ttcgtgaaaa
     2101 tacattttca gacttacggg atttcgcatt tcgagcaggg tggccgccgc atcgagctga
     2161 tgcccggcga ctgcctcgcc tacgatgtat cctgtccgca cacgatcgtc agcccgacgc
     2221 tgacgcgaca cgaggtcgtc atcgttccga aggaactgct gcaggaacgc ggcttccgcc
     2281 ttgcgaagat gacggcatgc aaattctcgg cgcgcaccgg caccggccgg atcgcctata
     2341 actttgtcca tgccgcgttc gacgaagcga ccaagctgta gccgaacaac gcgatcgggg
     2401 tggccgattc gctgatcgac ctcctgctgt tgccgctccg cgaacccgac gcgatgttca
     2461 atcgtgtcgg gcccgaggcc atgaatgtcc aggcgcggtt cttcatccgg gagcatctgc
     2521 gcgacccgga tctttcgatc gaccagatct cggcggcgct gaactgcacc aagcgctatc
     2581 tgcacatgct gttcagcgac aggggcacca ccatcagcga ctatatccag aacgcaaggt
     2641 tgcagaactg ccgccaggag ctggagaccc aagccggaaa acccatcacg gatgtcgcat
     2701 tttcgtgggg cttttcgagt tcgtcgcact tcagccgcgt attccggaaa cattttggag
     2761 ttattccgtc tgcgattcac aaagcgcaac acggtggtcc gtacccggac cttccttgcg
     2821 aatagaactt cggcgcggcg agcagatatt agccttgcac gcttgctggc ggaggctctc
     2881 ccgcatcaaa gcctggacgc acgaactcag ggggacggtt acgcaaaccg acagcgcggt
     2941 agagggacgc ccgattctca cctgacgccc tcaagggcgt tctggcgtcg gtcgattgtc
     3001 tttgaagtcg tcggcaaaac gcctgccacg gctcgatgca agaattccga tcgagccggg
     3061 ccggtcgttt cgatgcggtc tatttccgcg ctgcgcaggc gtacatgttg atttccatgc
     3121 ccaccggcac ctcgacaact ttcggagtct tccaggtcat tccatcctcc caggaaattt
     3181 ggcgtgaact ccacgccgag gttgaaccta ggaccacgcc cgacactgcg caagtgcgga
     3241 agatcgcgtc gaacacctgc gggccaggcc tgcagccgat atttctctcc tggacaaagc
     3301 gatctctctc ttggacaagg tcggccgttg cgatggtgcg ttcgcgatcg agaatgccaa
     3361 ctccaaagcc ccgcattgtg ccatccagtt cgattgtagc catagcttgt ctcctccaaa
     3421 ccagttgccc ttgtggacat tctgccgttt acgattctat taccttcaga gcttcccccg
     3481 catt
//
LOCUS       DATMGX010001126         2199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_189546, whole genome shotgun sequence.
ACCESSION   DATMGX010001126 DATMGX010000000
VERSION     DATMGX010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189546"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            551..766
                     /locus_tag="VNR65_18425"
     CDS             551..766
                     /locus_tag="VNR65_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20697.1"
                     /translation="MRSELVFEAVHTLRNRYTLCQLASKATRKFHKPNTRIQDTTNDV
                     LNLIADSERQDILIEPETASEAQRRAA"
     gene            839..2089
                     /gene="rho"
                     /locus_tag="VNR65_18430"
                     /pseudo
     CDS             839..2089
                     /gene="rho"
                     /locus_tag="VNR65_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
BASE COUNT          520 a          628 c          605 g          446 t
ORIGIN      
        1 gtaacggttg tgcgaatttt gataccctga aaggaagcaa cttattgata tccagcgggt
       61 taggaaaata ttgcggacct ttacggcgat gccgcgtcta agcgagaggc tggaggttac
      121 caaagtgaca atcgcgggga agattttcct tgactcatga gattcggacg actaccttac
      181 tgttttcaac gtaaggcgtc gtcccgtagg tatcagtcca aggcgtcctg ccccgaaaac
      241 ggcgacacac tgaacgccat catcaggcaa tcaggaagtc atctcccggc aagagcatgg
      301 tgctgagcta tgacgcgcgt cacgtaagta attgtttcag aaggttttgc agttttgcaa
      361 caagggccgg acggaaaagg aaaaaaatga ggaaccgaat cggcgactgc ggcatccaac
      421 gtttagctcc tgtcacgggc cgtcgtaatc ggtaatcctt taccgcccat tttttcatat
      481 gcaggagctg tggttctatt ctggccgggc tccgggaaca ttcattcata gaaacgagga
      541 gaacaaattg atgcggtctg aactagtatt tgaggctgtc cacacactgc gcaaccgcta
      601 cacgctgtgt cagcttgctt cgaaagcaac gcgcaagttc cacaaaccca acacgcggat
      661 tcaggacact accaacgacg tgttaaacct aatcgccgat tccgaacggc aggacatact
      721 gatcgaaccg gagacggctt ccgaagcgca gcggcgcgca gcataacgcg ccgcgccttc
      781 ggcgcgcttt ccgcagttcc gccggcccgc ccttctgcac attcccccgt aggtgaacat
      841 gacaatctca gagttaaaag aaaaaaacat caccgagctg accaaaattg ctcggaccct
      901 cgagctgccc ggcgccagtg gcctgcgcaa gcaggacctc atcttcaaga tcctgcaggc
      961 ccaaagcgaa aaagaaggcc atatttttgc cgagggcgtg ctggaaatcc tgcccgatgg
     1021 ctacggtttt ctgcgctcac ccgattacaa ctacctgccc ggtccggatg acatttacgt
     1081 ttctccctcg cagatccgca agtttgacct gaaaaccggc gacaccatca gcggccaggt
     1141 gcggcctccg cacgaaggcg agaagtattt cgcgctggta aagatcgagg ccgtcaactt
     1201 tgagtctccc gaggaagcgc gcaacaagat cctgttcgac aacctgacgc ctttgtatcc
     1261 gcaggagcgg atcaagctgg agacggtgcg cgaaaacatc agcgctcgcg tcatggacct
     1321 gctgacgccg gtgggcaaag ggcagcgcgg tttgatcgtg gcgcctccgc gcaccggcaa
     1381 gaccatgctc ctgcaaaacc tggcgaactc ggtcaccacc aatcatccgg aaatcgttct
     1441 catcgtgctg ctcatcgacg agcgtccgga agaagtgacg gatatgcagc gctcggtcaa
     1501 aggctaggtc atctcttcaa catttgacga gccggcagcc cgccacgttc aggtggccga
     1561 gatggtcatc gagaaggcca agcgcctggt cgagcacaag cgcgatgtcg tcatcctgct
     1621 cgattccatt acccgtctgg cgcgcgccta caacaccatt gtcccgccct ccggcaaggt
     1681 gctctctggc ggcgtcgatt ccaatgcgtt gcagcgtccg aagcggttct tcggcgccgc
     1741 ccgcaacatc gaagaaggcg gttcactgac cattattgcg acggccctca tcgaaaccgg
     1801 ttcgcgcatg gacgacgtca tcttcgaaga gttcaagggc accggcaaca tggaaatcat
     1861 tctcgaccgc aagctggtgg acaagcgtac cttcccggcc atcgacattc agcgctcagg
     1921 cacgcgtaag gaagagttgc tgattccgaa ggaagatctg gcgcgcgtct gggtgctgcg
     1981 caaagtattg aacccgctgt cgccggtgga agccatggaa ctgctgatcg acaagttggg
     2041 caagacccgt tccaacggcg aattcctgtc gaacatgagt gcgttgtaag gaagctgtca
     2101 gccgtcagct atcagctttc agttatggcg gcacgcaaca atttggatcg aggtgagggc
     2161 gcggaagcct gaaggtttcc gcgcctcttt gttgtgcac
//
LOCUS       DATMGX010001127         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_370597, whole genome shotgun sequence.
ACCESSION   DATMGX010001127 DATMGX010000000
VERSION     DATMGX010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370597"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(518..979)
                     /locus_tag="VNR65_18435"
     CDS             complement(518..979)
                     /locus_tag="VNR65_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20698.1"
                     /translation="MGISIGSVIVSSLELRHSLKSEMFGRDKGFKCPNLRWTAPGFQV
                     IKGIIHAPCAKPALRPESRDALLTAISKARGWINDIRLGRIPARAGVVRAFLVCGARG
                     AGAVDGYGRGVPAERDGFALWRRINPDKTGCTSRFLSVRHYSRDGCLHAWL"
BASE COUNT          508 a          647 c          650 g          444 t
ORIGIN      
        1 cgaggatcag aagggcgatg gcactgacgc tgacgcattt gctgatttcg gccagaggtt
       61 ggttcatggc gtcgatactg acttccggga cgaccagggc ggcgtcggtg ccgcgcgccg
      121 ggcagatggc gctgaacaca ggtaggccca ggtgaaccac cgatcgcgcc ggatgcgcgg
      181 acgcgtgccg tgcttggccc agatgcgggt cagggtggct tgctggccaa cgcgggcttc
      241 atccttacat gaaggtccct ttgtcaattc atattactgt cggattgatg gatctccgtg
      301 cagatccctg tcacggcggc ttggaggtcg gggtcgtttg ctgaacagcg agcgctgcat
      361 caccttacat acggtccggg tcagtgaccc ggcaaccaga atgcctcatg cacgaggcgt
      421 cgaccacggt taaatgtgac ccacctgaaa cgcgggcagc ataagctccc aatgaggaag
      481 acggttacat ttccccggac agccgccgac agagtaatta cagccacgca tgaaggcatc
      541 catcgcgcga ataatggcgg acgctaagaa atcgggaggt gcatcccgtc ttgtcaggat
      601 tgatccgtcg ccagagtgcg aacccgtcgc gctccgccgg gacaccccgg ccatatccat
      661 cgacggcgcc ggctccccgc gctccgcaca ccaaaaaagc gcggaccact cctgctcgtg
      721 ccggaatgcg gccgagcctg atgtcgttga tccatcctcg ggctttggag atcgcagtga
      781 gcagagcgtc ccggctttcg ggtctcagtg ccggttttgc gcagggcgca tgaatgatgc
      841 ccttgatgac ctgaaagcct ggcgcggtcc agcgaaggtt cggacacttg aaacctttat
      901 ccctgccgaa catttcactt tttagactat gtctcaattc gagggaggaa acgatgacac
      961 ttccgatgga aatgcccaag aaatctccga cttcaacccg aggctttgcc tccatggacc
     1021 gagaaaaaca acgtgcgatt gcgcgaaagg gcggcgaaag cgtaccgaac gaaaaacgca
     1081 gtttttcgca aaatcgcaga ttggcggctg aagctgggcg aaagggcgga cagagtgtca
     1141 atcccgccat gcgtagcttt tcgcgcaatc acgcgctcgc ctcggaggcc ggccgtaaag
     1201 gcggacatgc ctcacgcccc gggccgaaaa aagttggcgg ccaaggatga ggcatccttc
     1261 gtaaagcgcg aactgggtag cgcggctcca ccgcgtttgc gttgaatcgc agccgacgcg
     1321 gtggcctgcc ttgagaccgt cttaaatttg ggggctacca gcggcggcgc ggtcaaaccg
     1381 cttcatcgat tggatcgcca agtggtcgcg ctcgatttca attgacggac atcccatgtt
     1441 aataaagaaa agatgggctt tcgctggacg tttgaccata gcctccgagc aggtctacgc
     1501 gagcgcaacg actgaagcat ccatttgtcc gcgctcatct aaagagccat cacccccaga
     1561 ggtccagtgc gtcgtactgt ccgtcctttc atcaaggaat tcaagacccg ctcggctaag
     1621 tcgtcggcaa ctcatccacg gccgctcggc gataccgaca atgatggctc gaagctgtcg
     1681 ttcttggatc tcaatgtttt cacgactcgc cagaacaaat ttgaagccga acacaaggag
     1741 gcgttgacag ctgccgacgc ggtttttgga gaaggcagtc cggtggcccc actcccaaag
     1801 gcgtctccgt tatccaacgc ccctgttggc caggtgttgc cgagtcatat cggcgaagac
     1861 gatctgctga ctgttcgatt aacagaaggt gatggaaaat ccctcagcgg gcggagcgtc
     1921 ggaaaagtca aaagctcttc ccccattcgt ctcaaaaagc caactctccg gcccgaaagt
     1981 gaagtagcag ggggttttct agaacggccc gctacaagtt tctcaccgga tatattgatc
     2041 gtttcactac ctcgccgaga ccgcagatca attcaaaagc gttgggttct caggaccgaa
     2101 ctcaaggccg gagagaaatg gaagcggcgc ttgtgcaagg ctgcacgctg gcccgcgcga
     2161 aatccattcc gtcaagcaac aggtgccaga acttgacctc attaagcgat aggatcgccg
     2221 agttcatcgt cgaccgatta ccggacgag
//
LOCUS       DATMGX010001128         3823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_138576, whole genome shotgun sequence.
ACCESSION   DATMGX010001128 DATMGX010000000
VERSION     DATMGX010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138576"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VNR65_18440"
     CDS             <1..348
                     /locus_tag="VNR65_18440"
                     /inference="COORDINATES: protein motif:HMM:NF015748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HWJ20699.1"
                     /translation="EEGEVVEQIIKSKADCLFIAMPTPRKERFLAAYRDHLGVPFIMG
                     VGGSLDVIAGHVQRAPPNMQSMGLEWLYRVYQEPRRMWWRYVKTNTLFAGILIRGMLN
                     QCRGGARGLSRLG"
     gene            364..1485
                     /gene="wecB"
                     /locus_tag="VNR65_18445"
     CDS             364..1485
                     /gene="wecB"
                     /locus_tag="VNR65_18445"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HWJ20700.1"
                     /translation="MSKRVFVLFGTRPEAIKLFPVVKELQAHSAAEAIVCVTAQHREM
                     LDQVLKLVNIIPDIDLDLMEKNQSLDKLSARILLSVGEALDRTRPDRVVVQGDTATAM
                     VGSLAAYYRRIPVSHVEAGLRSGNIYAPWPEEVNRKIVGSIADQHFAPTKLAAEVLLK
                     ENVPPERVHVTGNTIVDALLATREMIESDPAHASRWDDLKLRAGDRRIILVTTHRREN
                     FGGGMDRIADALLTVLQRREDVMIVLPVHLNPNVRDVLEGRLAGHSRAEIIPPQEYAA
                     FVGLLSLAHIVLTDSGGIQEEAPSLGKPVLVMRDTTERPEGVLAGTARLVGTDRDRIV
                     SEIFSLLDEPNRYLEMSRAHNPFGDGHASKRIVKVMLDG"
     gene            1478..2749
                     /gene="wecC"
                     /locus_tag="VNR65_18450"
     CDS             1478..2749
                     /gene="wecC"
                     /locus_tag="VNR65_18450"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HWJ20701.1"
                     /translation="MAEFENVVVVGLGYIGLPTAAVIASRGMQVIGVDVNERVVNTVA
                     SGSIHIAEPDLEGLVQKVVSSGSLKVATEPRAADVFIIAVPTPIDEQKRPDLKSVMSA
                     VDSIAGVLRAGNLVILESTVPIGTTEQMAKKIAHVRPDLTVGMSEGSEHAISVAYCPE
                     RVLPGRILSELVHNDRCIGGVTPNCARRAQRFYKSFVRGACIATSARAAEMVKLTENA
                     FRDTNIAFANELSLICDRYGINVWEVIDLANRHPRVNVLRPGPGVGGHCIAVDPWFIV
                     DSAPDLARLIKTSREVNDSKISWTVQRAAALIEEHPYANVACCGLAFKANVDDLRESP
                     ALEVAHQLACRYGSRIKVVEPYIRKLPPSLAEHGAELTELDEAIRTCEIAVVLVDHDQ
                     FKMIPLGERRHLAVIDTRGIWQDMPLVGGPQ"
     gene            2761..3639
                     /locus_tag="VNR65_18455"
     CDS             2761..3639
                     /locus_tag="VNR65_18455"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWJ20702.1"
                     /translation="MHFLDLKGLKFFSVPSNFIACLLLVGLVLLLTFRSRSAALVLVS
                     GAIALCVAALSPLGNVLLTPLEQRFPTMQYPDRGIDGIIVLGGSYDTVSHGYMSTIVL
                     EEDSEPMAVMVDLARRYPKARIIFSGGTDPSFSGPSEASIVKQYFVSFGIPAERISIE
                     ERSQTTKENAQFTANLIHPTPSSRWLLVTSAYHMPRAVGAFRKAGFNVIAFPVGERTH
                     GWRDMWWPEDTATDNLRRVDIAVHEWLGLVVYHIRGYSSDWLARPVPRLRDDVLRPSV
                     TGGGSPRSAAMSRSDH"
BASE COUNT          781 a         1010 c         1146 g          886 t
ORIGIN      
        1 gaggagggcg aggttgtcga gcaaatcatc aagagcaaag cagattgcct tttcattgcg
       61 atgcccacac ctcgcaagga gcgttttctc gcagcttacc gagaccacct aggtgtgccg
      121 tttatcatgg gcgttggcgg atcgcttgac gtcatcgccg gccacgtgca gcgagctccc
      181 cccaatatgc agtcgatggg gctggaatgg ctttatcgtg tctaccagga gcctcgtcgt
      241 atgtggtgga ggtacgtcaa gacaaacacc ctttttgccg gaatactgat ccgtgggatg
      301 ctaaatcaat gcaggggtgg cgctcgcggg ctgtctcgtc tcggctgaga aagcaaaccg
      361 gagatgtcta agcgagtttt tgttctgttc ggaacgcggc ccgaagccat caaactcttc
      421 ccagttgtaa aggaattgca ggcgcatagc gcggcagagg cgatcgtgtg tgtcaccgcg
      481 caacatcgcg agatgctgga tcaggttctc aagctcgtaa atatcattcc cgatatcgat
      541 cttgacttga tggagaagaa ccaatctctg gacaagctaa gcgcccggat cctgctttcg
      601 gtcggcgaag cgctcgatcg cacccgtccg gacagagtgg tcgttcaggg tgacaccgct
      661 accgcgatgg tcggctcgct tgcggcgtac taccggcgga ttccggtctc gcacgtcgag
      721 gcaggcctcc gtagtgggaa tatttatgcg ccgtggcctg aggaagtgaa ccgtaagatc
      781 gttgggtcaa tcgcagacca acattttgct ccgaccaaac ttgcggcgga agtgctgttg
      841 aaagaaaatg taccccctga aagggtgcat gtgactggca atacgattgt tgacgcgttg
      901 ttagccacgc gagaaatgat tgagtccgat cctgcgcatg cctcccgctg ggatgacctt
      961 aagcttcgcg cgggtgatcg tcgcatcatt ctggtgacca cgcatcggcg cgagaatttt
     1021 ggcggcggaa tggatcgaat tgcggacgcg ctcttaacag ttttgcagcg gcgcgaggac
     1081 gtaatgatcg ttctgccggt gcacctcaat ccaaatgtcc gagatgtatt ggagggacgt
     1141 ttggccgggc attcacgggc tgaaatcatc cctcctcagg aatatgcagc ctttgtgggg
     1201 ctgctttctt tggcgcatat tgttctgact gactccggag ggatccagga agaggcgcct
     1261 tcgctcggaa aaccagtgct ggtgatgcgg gacacgactg agcggccgga aggtgtactg
     1321 gccgggacgg cgcgtttggt aggaactgac cgcgatcgga ttgtgagcga gatattctcc
     1381 ctgctggacg agccgaaccg ttacttggag atgtcgcgtg cacataatcc atttggagac
     1441 ggtcatgcga gtaagcggat agtgaaggtg atgctagatg gctgaatttg agaacgtcgt
     1501 tgtggtgggg cttgggtaca ttggtttgcc caccgctgcc gtcattgcta gccgcggaat
     1561 gcaggtcatc ggagtggacg tcaacgagcg tgtggtaaac acagttgcgt ccggttcgat
     1621 ccacattgcc gagcccgacc tggaaggtct ggtacagaag gttgtttctt ccggatcgct
     1681 caaggtggcg acggagcccc gggcggcaga cgtattcatc atcgccgtcc cgacaccaat
     1741 cgacgagcag aagcgtcctg atctgaaaag tgtcatgagc gctgtcgaca gcattgccgg
     1801 ggtactgcgt gcgggcaact tggtgatctt ggaatcaaca gttccgatcg gaacgacgga
     1861 acagatggcg aagaagatcg cgcacgttcg tcccgatctg acggtcggca tgtccgaggg
     1921 atccgaacac gctatttcgg tcgcgtattg ccccgagcga gtcttgccgg ggcgtattct
     1981 gtctgagcta gtacacaacg atcgttgcat cggcggggtg acgccgaact gcgccaggcg
     2041 cgcgcaaagg ttttacaaga gcttcgtcag gggcgcatgt atcgcaacga gcgcgcgtgc
     2101 cgcggagatg gtcaagctga ctgagaacgc attccgcgat accaacatcg cgtttgctaa
     2161 cgagctgtct ttgatctgcg atcgctacgg catcaatgta tgggaggtga ttgacctcgc
     2221 taaccgacat ccgcgcgtca atgtcttgcg cccgggcccg ggcgtcggcg gtcattgcat
     2281 tgcggtcgat ccgtggttca ttgtcgattc ggcgcccgat ctcgcgcgtc tgatcaagac
     2341 aagccgggag gtgaacgatt cgaagatttc ttggacagtt cagcgggcgg ccgcactgat
     2401 cgaagagcat ccctatgcca atgtcgcttg ttgtggcttg gccttcaagg caaacgtcga
     2461 cgatctgcga gagagccccg cgctggaggt tgcgcatcag ctggcctgtc gctacggatc
     2521 gcgcattaag gtcgtcgagc cgtacatccg caagctgccc ccaagcctcg cggaacacgg
     2581 agccgaattg accgagctcg atgaagcaat tcggacgtgt gagattgctg ttgtgctcgt
     2641 cgaccacgac cagttcaaga tgatcccgct aggtgaaaga cggcacctgg ccgtgatcga
     2701 tacaaggggg atttggcaag atatgccgtt ggtagggggc cctcagtaag gagaagctcg
     2761 atgcattttc ttgacctgaa agggcttaag tttttctctg ttccttcgaa ttttattgcg
     2821 tgcctattgt tggtcggcct tgttctactg ctgaccttcc gttcgcgctc tgcagcactc
     2881 gttttggtca gcggagcgat cgcgctctgc gtcgccgcgc tgtccccgct tgggaatgtc
     2941 cttttgacgc ctctcgaaca gcgcttcccg acaatgcagt atccggatcg agggattgac
     3001 gggatcatcg ttctcggggg ctcatacgat accgttagcc atggatacat gagcactatc
     3061 gtacttgaag aagatagcga accgatggcc gttatggtcg acctcgctcg ccgttatccc
     3121 aaggctcgca tcattttctc cggcggcacc gacccgtctt tctcggggcc gagcgaggca
     3181 tccattgtga agcaatactt tgtttcgttc ggaatccccg cagagcgcat ttcgattgaa
     3241 gaacgatcac agaccacgaa agaaaacgcc cagtttacgg ccaatttgat ccaccccacg
     3301 ccctcgtcac gctggctgct ggtgacatcc gcctaccaca tgccgcgcgc agtcggagcc
     3361 ttccggaagg ccggtttcaa cgtaatcgca tttccggtcg gggagcggac gcatggctgg
     3421 cgcgacatgt ggtggcctga ggatactgct acggacaatt tacgacgtgt ggatatcgcc
     3481 gtgcatgaat ggcttggttt ggtcgtctat catattagag gttattcttc cgattggctc
     3541 gcccgccctg tcccgagact cagagacgat gtcctaagac ctagtgtaac tggcggcggg
     3601 tcaccgcgat ctgcggcaat gagccggtca gatcattagc cgcagccgcc gccctggcaa
     3661 attcgaacta aaccattgca gcgctactca ccctagtgac tagggtgatg ggtattatcc
     3721 ttattttcgt cttttcctat acaccttcgg acagttgaat tatctcttct gagcttagtt
     3781 tggagtgtca attcatctgt gccagcgtgc gggtcagggg gaa
//
LOCUS       DATMGX010001129         3142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Geobacterales bacterium isolate SMAG_U13500
            k141_460040, whole genome shotgun sequence.
ACCESSION   DATMGX010001129 DATMGX010000000
VERSION     DATMGX010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942903
            Sequence Read Archive: SRR8090375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Geobacterales bacterium (soil metagenome)
  ORGANISM  Geobacterales bacterium
            Bacteria; Thermodesulfobacteriota; Desulfuromonadia; Geobacterales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 16:53:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,694
            CDSs (total)                      :: 3,674
            Genes (coding)                    :: 3,622
            CDSs (with protein)               :: 3,622
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 15 of 52
            Pseudo Genes (incomplete)         :: 45 of 52
            Pseudo Genes (internal stop)      :: 7 of 52
            Pseudo Genes (multiple problems)  :: 12 of 52
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Geobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460040"
                     /isolate="SMAG_U13500"
                     /isolation_source="soil"
                     /db_xref="taxon:3069300"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1715
                     /gene="istA"
                     /locus_tag="VNR65_18460"
     CDS             186..1715
                     /gene="istA"
                     /locus_tag="VNR65_18460"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HWJ20703.1"
                     /translation="MELFEEIRREHAHGAGTIRAVAKKLGVHRRMVRQALASSIPPER
                     KLAARNKPRLGPVMEFIDEILREDQTAPRKQRHTAHRIWERIRQERSDTVAEATVRRY
                     VREGKQKLGLTGRETFVPQVYDWGGEAQVDWYEAMADFAGERLRVYIFAMRSMASGGA
                     FHVAYYHATQQAFLEAHELAFAYFGGVFRCLRYDNLKSAVKKILRGHQREETERLIAF
                     RSHWGFQTEFCNPARGNEKGGVEGEVGYFRRNHLVPIPQAQNLEELNEQLRSYCQQDE
                     QRRIAGKAMPVGEALRIEREHLLPLAAEGFELAETSFPTVDSKGCVKVRTNFYSTPLR
                     PGTRPQAKLLPAYVEIWQERECVARHERSFGRFEQVLDLEHYLDVLEKKPGALAGSRP
                     LRQWRERGRWPESFDRLWLSLQERHGKQVGTREMIELLTLGKRCGWDRLQQAVEKALG
                     LGCTDAAAVRHLLTATELSRPRNELLELNGLERYQRPLPLMNEYDQLLSIAASTAEVA
                     R"
     gene            1712..2491
                     /gene="istB"
                     /locus_tag="VNR65_18465"
     CDS             1712..2491
                     /gene="istB"
                     /locus_tag="VNR65_18465"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HWJ20704.1"
                     /translation="MKEQTQALEHASVKQYCKALRVPMIGAHFIQLAEQAIKEQRSHI
                     GYLEALLTMESEERDRHAVQQRLRDAKLPRLKTLEEFDFNQARQIPAAKIRELAEGGY
                     IERAEPVVLMGECGTGKTHLATGLCVAACRQKRRARFTTAANLVNELVEARQHNAVKR
                     VLARWLRYDVIVLDEVGYVPLADIGAEFLFQVIAERAERAALIVTTNLPFSEWTQVFP
                     NPRLCKALLDRITDRAHIIETGAESYRFRRTLEVRQKKKAS"
     gene            2787..>3142
                     /locus_tag="VNR65_18470"
     CDS             2787..>3142
                     /locus_tag="VNR65_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWJ20705.1"
                     /translation="MLRLDKVIKPWKESAALNDHINLYGFWNETAFLTKSGDVGMVLS
                     VPGVDYESLDRSEQEYAVKRLEAALKAFGPGFHVYQYLFKSNRPDIPFAKYDDPIVEA
                     AIEQRRKFFEAKRDHL"
BASE COUNT          684 a          858 c         1041 g          559 t
ORIGIN      
        1 atggctctca tcgaacaact cggcgttcac acccacaatt aaggaggcga cggtcgctat
       61 gccggtgatt tcgctactct gaagtggccc cactttgatc attacatttg gccccaccct
      121 aaccgataga tcggcctacc ctgtctgcga agcagacggg gagggcgatg gacaggagac
      181 gcaaggtgga gctattcgaa gagatcaggc gggaacatgc gcatggagcg ggcacgattc
      241 gtgcggtcgc gaagaaactg ggcgtgcatc ggcgaatggt gcgacaggcc ctggctagtt
      301 cgattccgcc ggagcgcaaa ctggcggcgc gaaacaagcc gaggctgggt ccggtgatgg
      361 agttcattga cgagattctg cgcgaggatc agacggcgcc gcgtaaacag cggcacacgg
      421 cacatcggat ctgggaacga atccggcaag agcgctcgga caccgtggcg gaagcgaccg
      481 tgcgccgtta tgtgcgggaa ggcaagcaaa agttaggact cacggggcgg gagacattcg
      541 tgccgcaagt ctacgactgg ggcggcgagg cgcaggtgga ttggtacgaa gccatggccg
      601 acttcgcggg cgaacgattg cgggtttaca tttttgcgat gcgttcgatg gccagtgggg
      661 gagcgtttca cgtggcctac tatcacgcca cgcagcaggc ttttttggag gcgcacgagc
      721 tggcattcgc gtatttcggc ggcgtgttcc gctgcttgcg ctacgacaac ctgaagagcg
      781 cggtgaagaa gattctgcgc gggcatcagc gggaggaaac ggagcggctg atcgcgttcc
      841 gctcgcactg gggctttcag acggagttct gtaatccggc gcgaggcaac gagaagggcg
      901 gcgtggaggg cgaggtaggt tacttccgcc ggaaccatct ggttccgatt ccgcaggcgc
      961 agaacctgga agagctgaac gaacagttac gaagttattg ccagcaagac gagcagcggc
     1021 ggatcgcagg caaagcaatg ccagtcggtg aggcgctgcg gatcgaacgc gagcacctgc
     1081 tgccgctggc ggcggaagga ttcgaactgg cggagaccag tttcccgacg gtggatagta
     1141 agggctgtgt gaaggtgcgc accaacttct actcgacgcc attgaggcca gggacgcgcc
     1201 cgcaagcgaa actgctgcca gcgtatgtcg agatctggca ggaacgggag tgcgtggcac
     1261 ggcacgagcg cagtttcggc cgctttgagc aagtgctcga tctggagcac tatctcgacg
     1321 tgctggagaa gaaaccggga gcgctggcag gttcacggcc gttgcggcag tggcgcgaac
     1381 gcggacgctg gccggagagt ttcgaccggc tctggctgag cttgcaagaa cggcacggca
     1441 agcaggtggg cacgcgggag atgatcgagt tactcacgct cggcaagcgc tgcgggtggg
     1501 atcggctgca gcaggcggtg gaaaaagctc tgggcttggg ttgcacggat gcggccgcgg
     1561 ttcggcactt gctgacggca acggaactta gtcgtccgcg caacgagttg ctcgaactga
     1621 atggactgga acgctaccag cgaccgttgc cgctgatgaa cgagtacgac cagttactca
     1681 gcatcgctgc cagcacggcg gaggtggcgc gatgaaagag caaacccagg ctctcgaaca
     1741 cgccagcgtc aagcaatatt gcaaagcgct gcgcgttccg atgatcggag cgcacttcat
     1801 acaactggcg gagcaagcca tcaaagaaca gcgcagtcac atcggctatc tggaagcgct
     1861 gttgacgatg gaatccgaag agcgcgaccg gcacgccgtt cagcaacggc tgcgcgatgc
     1921 caagctgccg cggctgaaga cgctggaaga gttcgacttc aaccaggcgc ggcagattcc
     1981 ggcggcgaag attcgcgaac tggccgaagg cggctacatc gaacgcgccg aacccgtggt
     2041 gctgatgggc gaatgtggca ccggcaagac gcacttggcg acgggcctgt gcgtggccgc
     2101 gtgccggcag aaacggcgag cgcgattcac caccgctgcc aatctggtga acgaactggt
     2161 ggaagcccgg caacacaacg ccgtgaagcg agtgctggcg cgctggttgc gctacgacgt
     2221 aatcgtgctc gatgaagtcg gctacgtgcc gttggccgac atcggcgcgg aattcttgtt
     2281 tcaggtgatt gccgaacggg cagaacgagc cgccctcatc gtgaccacga acctgccgtt
     2341 ttccgaatgg acgcaggtgt ttcccaatcc gcgcttgtgc aaggcgttgc tcgaccgaat
     2401 cacggaccga gcgcacatca tcgaaaccgg cgccgagtcc tatcgctttc gcagaaccct
     2461 ggaggtgcgg cagaaaaaga aagcatcgta gcggccgacg gagtagcttc tacattgctg
     2521 gtgcaaagga aggggctcaa acgccgcccc ttcctctgca ctccatcccg gctgggatcg
     2581 taagactctg tcgaaccggt ccggcttcct tattaatgag gtggggccaa ttgtaagtat
     2641 caaggtgggg ccaaatcagg ttgtcaaacg cagcattggg tcacgaacag cgatccggcg
     2701 tttctccgca tcttggcgaa gtcggaacgc tacaagctgc ggtatgacgc ggccaagcag
     2761 ttgatccccc gcgtggaggt gttgtagtgc tccgtctcga taaggtcatc aagccgtgga
     2821 aagaaagtgc ggctctcaac gaccacatca acctgtacgg gttctggaat gagacggcat
     2881 tcctcaccaa gtctggcgat gtcggcatgg tactcagcgt tcccggcgtg gactacgaaa
     2941 gcctggaccg ctcggagcag gagtatgcgg tcaagcggtt ggaggccgct ctgaaagcct
     3001 tcggcccagg tttccatgtt tatcaatatc tcttcaagtc gaaccgcccc gacataccct
     3061 tcgccaaata cgacgacccg attgtggaag cggccatcga gcagcggcgg aagttctttg
     3121 aggcgaagcg cgaccatctt ta
//
